@22:49:42.35CEST23-May-19
>RESET< ESP2 v3.30 I2C Gateway v3.06 for core bus @22:49:44.44 Copyright 2018 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @22:49:44.75 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @22:49:50.95 Polling Can every 10:00 @22:49:51.00 Loading /home/esp/esp2/mission/190523totalPostcanqc.rb mission @22:49:51.07 Preparing to sleep until +0:00:15 @22:49:51.09 GoodNews.email "Starting 190523totalPostcanqc w/pucks in tube 2\n{}\nCan@22:49:50, 20.3C, 30% humidity, 14.6psia, 12.055V, 0.317A, 0.317A avg, 3.82W",Subject: "190523totalPostcanqc begun" @22:49:51.19 Shutdown @22:49:51.22
Asleep til +0:00:15 @22:49:51.23 Continuing at 22:50 @22:49:54.64 Session Begun @22:49:59.32 -> showlog 100 @22:49:59.94 GoodNews.email "Then, in tube 2: mbaPhase\n/home/esp/esp2/mission/190523totalPostcanqc.rb:111\n{}\nCan@22:49:51, 20.3C, 30% humidity, 14.6psia, 12.055V, 0.317A, 0.317A avg, 3.82W",Subject: "Asleep until 22:50" @22:50:06.04
Powering core bus @22:50:06.99 Session Begun @22:50:11.07
Gate.power :raw,:ON @22:50:11.77 SPE.coast @22:50:11.87 SPE.configure SPEconfig @22:50:12.02 AS.configure ASconfig @22:50:12.16 SS.configure SSconfig @22:50:12.30 CC.configure CCconfig @22:50:12.43 CS.configure CSconfig @22:50:12.57 PC.configure PCconfig @22:50:12.71 PS.configure PSconfig @22:50:12.88 Forearm.configure LooseForearm @22:50:13.01 Elbow.configure LooseElbow @22:50:13.17 SC.configure SCloose @22:50:13.30 SE.configure SEconfig @22:50:13.38 CH.coast @22:50:13.48 CH.configure CHconfig @22:50:13.55 PH.coast @22:50:13.65 PH.configure PHconfig @22:50:13.73 PCR.off @22:50:13.78 PCR.pidtemp= 50,0.1,0,300,25,25 @22:50:13.84 PCR.caltemp= 2.8814,0.0372,0 @22:50:13.92 All dwarf controllers running firmware version 3.75 @22:50:14.08 Valve.reset @22:50:14.30 PCR.off @22:50:14.36 PCR.pidtemp= 50,0.1,0,300,25,25 @22:50:14.42 PCR.caltemp= 2.8814,0.0372,0 @22:50:14.55 Intake.select :close @22:50:14.63 Exhaust.select :close @22:50:14.69 CTV.dial :air @22:50:14.88 PSV.dial :air @22:50:15.03 CSV.dial :waste1 @22:50:15.07
Forearm.reconfigure LooseForearm @22:50:15.25 Forearm.home.seek :retract @22:50:15.35 SSV.dial :spit,avoiding: :exhaust @22:50:15.38 PS.reconfigure PSconfig @22:50:15.55 PS.home.seek 0ml @22:50:15.71 CS.reconfigure CSfast @22:50:15.75 SS.reconfigure SSconfig @22:50:15.90 CS.home.seek 0ml @22:50:16.03 SS.home.seek 0ml @22:50:16.15 Hand.close @22:50:16.23
Elbow.jog 1500 @22:50:16.99 Elbow.reconfigure LooseElbow @22:50:17.08 Elbow.home.seek :clear @22:50:20.45 SE.reconfigure SEconfig @22:50:20.54 SE.home.seek :down @22:50:21.41 SC.reconfigure SCsloppy @22:50:21.50 SC.home.seek :initial @22:50:24.93 PS.coast @22:50:25.55
Forearm.seek :retract @22:50:25.68 Elbow.seek :hammer @22:50:26.92 Forearm.reconfigure TightForearm @22:50:27.00 Forearm.seek 5 + 350 counts @22:50:28.14 Forearm.seek :retract @22:50:29.36 PC.reconfigure PCconfig @22:50:29.38
CC.reconfigure CCconfig @22:50:29.51 PC.home.seek :open @22:50:30.04
CC.home.seek :open @22:50:33.34 GoodNews.email "At 22:50:33 began in tube 2: mbaPhase\n/home/esp/esp2/mission/190523totalPostcanqc.rb:111\n{}\nCan@22:50:33, 20.3C, 30% humidity, 14.6psia, 11.859V, 0.985A, 0.985A avg, 11.68W",Subject: "Started 190523totalPostcanqc phase mbaPhase" @22:50:33.38
Phase [mbaPhase] @22:50:33.45CEST23-May-19 ==================> STARTED MBAsuperphase (phase #1) <=== @22:50:34.19 Readying the MFB @22:50:34.20 Homing MFB Syringe @22:50:34.28 AFV.select :holdingCoil @22:50:34.50 ATV.dial :air2 @22:50:35.13 AS.reconfigure ASconfig @22:50:35.22 AS.home.seek :home @22:50:40.77 AFV.select :carrierSolution @22:50:40.91 delay 2 seconds @22:50:42.95 AS.seek 1ml @22:51:35.88 delay 1 second @22:51:36.90 AFV.select :holdingCoil @22:51:37.03 delay 1 second @22:51:38.06 ATV.dial :waste @22:51:39.25 AS.reconfigure ASnorm @22:51:39.34 AS.home.seek :empty @22:51:52.15 -> #I got the startup email. That's good! @22:52:31.95
ATV.dial :waste @22:52:31.96 ARV.dial between carrier and air1 @22:52:32.12 ASV.dial :waste1 @22:52:32.31 Starting mfb @22:52:32.33 pcrBAC sampling at most 1000ml, wcr at most 1000ml @22:52:32.44 BACfans.email "Started mfb in tube 2 at 22:52:32CEST23-May-19\n{}\nCan@22:50:33, 20.3C, 30% humidity, 14.6psia, 11.859V, 0.985A, 0.985A avg, 11.68W",Subject: "pcrBAC sampling at most 1000ml, wcr at most 1000ml" @22:52:32.51
Loading sh1 puck from tube 2 into Collection Clamp @22:52:32.67 Hand.open @22:52:33.68 SC.reconfigure SCloose @22:52:33.90 Elbow.reconfigure TightElbow @22:52:33.99 Elbow.seek 2 @22:52:34.46 Forearm.seek 2 + 700 counts @22:52:35.59 SE.reconfigure SEpick @22:52:35.70 SE.seek beamBreak @22:52:36.73 SE.configure SEconfig @22:52:36.98 Elbow.seek 2 @22:52:37.49 Forearm.seek 2 + 300 counts @22:52:37.79 Forearm.reconfigure LooseForearm @22:52:37.87 Hand.close @22:52:38.70 SE.seek :down @22:52:38.98
Forearm.seek :retract @22:52:39.63 SE.coast @22:52:39.96
Elbow.seek CC @22:52:42.09 Elbow.reconfigure LooseElbow @22:52:42.24 Forearm.reconfigure TightForearm @22:52:42.33 Forearm.seek CC @22:52:43.71 CC.seek :closed @22:52:48.03 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.44 @22:52:48.04 Hand.open @22:52:48.88 Forearm.reconfigure LooseForearm @22:52:48.97 Forearm.seek :retract @22:52:50.02 SC.coast @22:52:50.11 collectSample 1000ml w/bubble point of 55psi @22:52:50.12 Priming 7ml @22:52:50.16 Gate.power :sampler,:ON @22:52:50.23 Intake.select :close @22:52:50.24 Exhaust.select :close @22:52:50.27 SS.hold @22:52:50.59 Calibrating pressure and temperature sensors @22:52:50.61 CTV.dial :air @22:52:50.66 SSV.dial :spit,avoiding: :exhaust @22:52:50.74 SS.reconfigure SSfast @22:52:50.83 SS.seek 5ml @22:52:53.81 SSV.dial CBV @22:52:53.85
CBV.dial SSV @22:52:55.44 delay 3.8 seconds @22:52:59.38 SSV.dial :spit @22:53:00.95 SS.seek 0ml @22:53:03.81 SSV.dial CBV @22:53:05.34 CTV.dial :intake @22:53:06.26 Waiting for external sampler to finish priming... @22:53:06.27 delayUntil "22:57:50.21" @22:55:52.62 Session Ended @22:57:50.23 SSV.dial CBV @22:57:50.26
CBV.dial SSV @22:57:50.30 Allowing 40s for 7.0ml of remaining 7.0ml @22:57:50.33 Intake.select :open @22:57:52.55 Intake @15.6psia, vacuum from 9.1 to 13.1psi @22:57:52.57 SS.reconfigure SSinhale.with maxSpeed: 150 @22:57:52.67 SS.seek 7ml @22:57:58.43 Stroke completed -- waiting for pressure to equalize ... @22:58:12.85 Intake.select :close @22:58:13.03 SSV.dial :exhaust @22:58:13.92 Exhaust.select :open @22:58:14.05 SS.reconfigure SSexhale @22:58:14.16 SS.seek 0ml @22:58:18.45 Exhaust.select :close @22:58:18.60 Sampling 1000.0ml @22:58:18.62 Intake.select :close @22:58:18.66 Exhaust.select :close @22:58:18.69 SS.reconfigure SSconfig @22:58:18.78 SS.hold @22:58:19.53 CTV.dial :intake @22:58:19.55 SSV.dial CBV @22:58:19.59
CBV.dial SSV @22:58:20.51 Allowing 130s for 25.0ml of remaining 1000.0ml @22:58:20.53 Intake.select :open @22:58:22.75 Intake @15.5psia, vacuum from 9.0 to 13.0psi @22:58:22.77 SS.reconfigure SSinhale.with maxSpeed: 150 @22:58:22.87 SS.seek 25ml @22:58:26.53 Vacuum too high -- waiting up to 40s @22:58:31.87 Intake @15.3psia, vacuum from 8.8 to 12.8psi @22:58:31.89 SS.reconfigure SSinhale.with maxSpeed: 135 @22:58:31.99 SS.seek 25ml @22:58:35.15 Vacuum too high -- waiting up to 40s @22:58:40.99 Intake @15.3psia, vacuum from 8.8 to 12.8psi @22:58:41.01 SS.reconfigure SSinhale.with maxSpeed: 122 @22:58:41.11 SS.seek 25ml @22:58:43.90 Vacuum too high -- waiting up to 40s @22:58:49.99 Intake @15.4psia, vacuum from 8.9 to 12.9psi @22:58:50.02 SS.reconfigure SSinhale.with maxSpeed: 110 @22:58:50.12 SS.seek 25ml @22:58:53.75 Vacuum too high -- waiting up to 40s @22:59:00.33 SS.reconfigure SSinhale.with maxSpeed: 99 @22:59:00.41 SS.seek 25ml @22:59:04.19 Vacuum too high -- waiting up to 40s @22:59:10.55 Intake @15.4psia, vacuum from 8.9 to 12.9psi @22:59:10.57 SS.reconfigure SSinhale.with maxSpeed: 89 @22:59:10.67 SS.seek 25ml @22:59:14.50 Vacuum too high -- waiting up to 40s @22:59:21.08 Intake @15.4psia, vacuum from 8.9 to 12.9psi @22:59:21.11 SS.reconfigure SSinhale.with maxSpeed: 80 @22:59:21.21 SS.seek 25ml @22:59:23.24 Stroke completed -- waiting for pressure to equalize ... @22:59:27.69 Intake.select :close @22:59:27.87 SSV.dial :exhaust @22:59:28.76 Exhaust.select :open @22:59:28.91 SS.reconfigure SSexhale @22:59:28.99 SS.seek 0ml @22:59:38.60 Exhaust.select :close @22:59:38.75 SSV.dial CBV @22:59:38.78
CBV.dial SSV @22:59:39.70 Allowing 130s for 25.0ml of remaining 975.0ml @22:59:39.72 Intake.select :open @22:59:41.94 Intake @15.6psia, vacuum from 9.1 to 13.1psi @22:59:41.95 SS.reconfigure SSinhale.with maxSpeed: 80 @22:59:42.06 SS.seek 25ml @22:59:46.59 Vacuum too high -- waiting up to 40s @22:59:51.43 Intake @15.3psia, vacuum from 8.8 to 12.8psi @22:59:51.45 SS.reconfigure SSinhale.with maxSpeed: 72 @22:59:51.56 SS.seek 25ml @22:59:55.93 Vacuum too high -- waiting up to 40s @23:00:00.76 Intake @15.3psia, vacuum from 8.8 to 12.8psi @23:00:00.78 SS.reconfigure SSinhale.with maxSpeed: 65 @23:00:00.88 SS.seek 25ml @23:00:05.34 Vacuum too high -- waiting up to 40s @23:00:09.90 Intake @15.3psia, vacuum from 8.8 to 12.8psi @23:00:09.92 SS.reconfigure SSinhale.with maxSpeed: 59 @23:00:10.02 SS.seek 25ml @23:00:15.22 Vacuum too high -- waiting up to 40s @23:00:19.85 Intake @15.3psia, vacuum from 8.8 to 12.8psi @23:00:19.87 SS.reconfigure SSinhale.with maxSpeed: 53 @23:00:19.97 SS.seek 25ml @23:00:27.09 Vacuum too high -- waiting up to 40s @23:00:31.93 Intake @15.3psia, vacuum from 8.8 to 12.8psi @23:00:31.95 SS.reconfigure SSinhale.with maxSpeed: 48 @23:00:32.05 SS.seek 25ml @23:00:39.40 Stroke completed -- waiting for pressure to equalize ... @23:00:46.31 Intake.select :close @23:00:46.49 SSV.dial :exhaust @23:00:47.37 Exhaust.select :open @23:00:47.52 SS.reconfigure SSexhale @23:00:47.60 SS.seek 0ml @23:00:57.07 Exhaust.select :close @23:00:57.22 SSV.dial CBV @23:00:57.25
CBV.dial SSV @23:00:58.15 Allowing 130s for 25.0ml of remaining 950.0ml @23:00:58.17 Intake.select :open @23:01:00.40 Intake @15.6psia, vacuum from 9.1 to 13.1psi @23:01:00.42 SS.reconfigure SSinhale.with maxSpeed: 48 @23:01:00.51 SS.seek 25ml @23:01:09.15 Vacuum too high -- waiting up to 40s @23:01:13.23 Intake @15.2psia, vacuum from 8.7 to 12.7psi @23:01:13.25 SS.reconfigure SSinhale.with maxSpeed: 43 @23:01:13.35 SS.seek 25ml @23:01:23.03 Vacuum too high -- waiting up to 40s @23:01:27.36 Intake @15.3psia, vacuum from 8.8 to 12.8psi @23:01:27.39 SS.reconfigure SSinhale.with maxSpeed: 39 @23:01:27.49 SS.seek 25ml @23:01:44.81 Vacuum too high -- waiting up to 40s @23:01:49.89 SS.reconfigure SSinhale.with maxSpeed: 35 @23:01:49.97 SS.seek 25ml @23:01:58.87 Stroke completed -- waiting for pressure to equalize ... @23:02:03.30 Intake.select :close @23:02:03.48 SSV.dial :exhaust @23:02:04.36 Exhaust.select :open @23:02:04.51 SS.reconfigure SSexhale @23:02:04.59 SS.seek 0ml @23:02:13.79 Exhaust.select :close @23:02:13.94 SSV.dial CBV @23:02:13.97
CBV.dial SSV @23:02:14.87 Allowing 130s for 25.0ml of remaining 925.0ml @23:02:14.89 Intake.select :open @23:02:17.11 Intake @15.5psia, vacuum from 9.0 to 13.0psi @23:02:17.12 SS.reconfigure SSinhale.with maxSpeed: 35 @23:02:17.22 SS.seek 25ml @23:02:57.40 Vacuum too high -- waiting up to 40s @23:03:01.50 Intake @15.1psia, vacuum from 8.6 to 12.6psi @23:03:01.52 SS.reconfigure SSinhale.with maxSpeed: 32 @23:03:01.62 SS.seek 25ml @23:03:13.57 Stroke completed -- waiting for pressure to equalize ... @23:03:17.38 Intake.select :close @23:03:17.56 SSV.dial :exhaust @23:03:18.44 Exhaust.select :open @23:03:18.59 SS.reconfigure SSexhale @23:03:18.67 SS.seek 0ml @23:03:28.21 Exhaust.select :close @23:03:28.36 SSV.dial CBV @23:03:28.39
CBV.dial SSV @23:03:29.29 Allowing 130s for 25.0ml of remaining 900.0ml @23:03:29.31 Intake.select :open @23:03:31.53 Intake @15.5psia, vacuum from 9.0 to 13.0psi @23:03:31.55 SS.reconfigure SSinhale.with maxSpeed: 32 @23:03:31.66 SS.seek 25ml @23:04:26.60 Stroke completed -- waiting for pressure to equalize ... @23:04:30.41 Intake.select :close @23:04:30.59 SSV.dial :exhaust @23:04:31.48 Exhaust.select :open @23:04:31.63 SS.reconfigure SSexhale @23:04:31.71 SS.seek 0ml @23:04:40.94 Exhaust.select :close @23:04:41.09 SSV.dial CBV @23:04:41.12
CBV.dial SSV @23:04:42.04 Allowing 130s for 25.0ml of remaining 875.0ml @23:04:42.06 Intake.select :open @23:04:44.28 Intake @15.5psia, vacuum from 9.0 to 13.0psi @23:04:44.29 SS.reconfigure SSinhale.with maxSpeed: 32 @23:04:44.40 SS.seek 25ml @23:05:39.91 Stroke completed -- waiting for pressure to equalize ... @23:05:43.72 Intake.select :close @23:05:43.91 SSV.dial :exhaust @23:05:44.80 Exhaust.select :open @23:05:44.95 SS.reconfigure SSexhale @23:05:45.04 SS.seek 0ml @23:05:54.66 Exhaust.select :close @23:05:54.79 SSV.dial CBV @23:05:54.82
CBV.dial SSV @23:05:55.74 Allowing 130s for 25.0ml of remaining 850.0ml @23:05:55.76 Intake.select :open @23:05:57.98 Intake @15.5psia, vacuum from 9.0 to 13.0psi @23:05:58.00 SS.reconfigure SSinhale.with maxSpeed: 32 @23:05:58.09 SS.seek 25ml @23:06:52.96 Stroke completed -- waiting for pressure to equalize ... @23:06:56.14 Intake.select :close @23:06:56.81 SSV.dial :exhaust @23:06:57.69 Exhaust.select :open @23:06:57.82 SS.reconfigure SSexhale @23:06:57.89 SS.seek 0ml @23:07:07.66 Exhaust.select :close @23:07:07.81 SSV.dial CBV @23:07:07.84
CBV.dial SSV @23:07:08.74 Allowing 130s for 25.0ml of remaining 825.0ml @23:07:08.76 Intake.select :open @23:07:10.98 Intake @15.5psia, vacuum from 9.0 to 13.0psi @23:07:11.00 SS.reconfigure SSinhale.with maxSpeed: 32 @23:07:11.11 SS.seek 25ml @23:08:06.19 Stroke completed -- waiting for pressure to equalize ... @23:08:10.02 Intake.select :close @23:08:10.20 SSV.dial :exhaust @23:08:11.08 Exhaust.select :open @23:08:11.23 SS.reconfigure SSexhale @23:08:11.32 SS.seek 0ml @23:08:20.63 Exhaust.select :close @23:08:20.78 SSV.dial CBV @23:08:20.81
CBV.dial SSV @23:08:21.72 Allowing 130s for 25.0ml of remaining 800.0ml @23:08:21.74 Intake.select :open @23:08:23.96 Intake @15.5psia, vacuum from 9.0 to 13.0psi @23:08:23.98 SS.reconfigure SSinhale.with maxSpeed: 32 @23:08:24.08 SS.seek 25ml @23:09:19.24 Stroke completed -- waiting for pressure to equalize ... @23:09:23.05 Intake.select :close @23:09:23.23 SSV.dial :exhaust @23:09:24.12 Exhaust.select :open @23:09:24.27 SS.reconfigure SSexhale @23:09:24.35 SS.seek 0ml @23:09:31.02 -> exit @23:09:31.04 Session Ended @23:09:33.90
Exhaust.select :close @23:09:34.02 SSV.dial CBV @23:09:34.05
CBV.dial SSV @23:09:34.96 Allowing 130s for 25.0ml of remaining 775.0ml @23:09:34.98 Intake.select :open @23:09:37.16 Intake @15.4psia, vacuum from 8.9 to 12.9psi @23:09:37.17 SS.reconfigure SSinhale.with maxSpeed: 32 @23:09:37.22 SS.seek 25ml @23:10:32.27 Stroke completed -- waiting for pressure to equalize ... @23:10:35.45 Intake.select :close @23:10:35.61 SSV.dial :exhaust @23:10:36.47 Exhaust.select :open @23:10:36.59 SS.reconfigure SSexhale @23:10:36.63 SS.seek 0ml @23:10:46.16 Exhaust.select :close @23:10:46.28 SSV.dial CBV @23:10:46.31
CBV.dial SSV @23:10:47.18 Allowing 130s for 25.0ml of remaining 750.0ml @23:10:47.19 Intake.select :open @23:10:49.36 Intake @15.5psia, vacuum from 9.0 to 13.0psi @23:10:49.38 SS.reconfigure SSinhale.with maxSpeed: 32 @23:10:49.43 SS.seek 25ml @23:11:44.44 Stroke completed -- waiting for pressure to equalize ... @23:11:47.63 Intake.select :close @23:11:47.79 SSV.dial :exhaust @23:11:48.65 Exhaust.select :open @23:11:48.77 SS.reconfigure SSexhale @23:11:48.81 SS.seek 0ml @23:11:58.41 Exhaust.select :close @23:11:58.53 SSV.dial CBV @23:11:58.56
CBV.dial SSV @23:11:59.43 Allowing 130s for 25.0ml of remaining 725.0ml @23:11:59.44 Intake.select :open @23:12:01.62 Intake @15.5psia, vacuum from 9.0 to 13.0psi @23:12:01.64 SS.reconfigure SSinhale.with maxSpeed: 32 @23:12:01.68 SS.seek 25ml @23:12:56.86 Stroke completed -- waiting for pressure to equalize ... @23:13:00.54 Intake.select :close @23:13:00.69 SSV.dial :exhaust @23:13:01.57 Exhaust.select :open @23:13:01.69 SS.reconfigure SSexhale @23:13:01.74 SS.seek 0ml @23:13:11.24 Exhaust.select :close @23:13:11.36 SSV.dial CBV @23:13:11.39
CBV.dial SSV @23:13:12.25 Allowing 130s for 25.0ml of remaining 700.0ml @23:13:12.26 Intake.select :open @23:13:14.44 Intake @15.5psia, vacuum from 9.0 to 13.0psi @23:13:14.46 SS.reconfigure SSinhale.with maxSpeed: 32 @23:13:14.51 SS.seek 25ml @23:14:09.61 Stroke completed -- waiting for pressure to equalize ... @23:14:12.82 Intake.select :close @23:14:12.98 SSV.dial :exhaust @23:14:13.85 Exhaust.select :open @23:14:13.97 SS.reconfigure SSexhale @23:14:14.02 SS.seek 0ml @23:14:23.25 Exhaust.select :close @23:14:23.37 SSV.dial CBV @23:14:23.40
CBV.dial SSV @23:14:24.27 Allowing 130s for 25.0ml of remaining 675.0ml @23:14:24.28 Intake.select :open @23:14:26.46 Intake @15.5psia, vacuum from 9.0 to 13.0psi @23:14:26.47 SS.reconfigure SSinhale.with maxSpeed: 32 @23:14:26.52 SS.seek 25ml @23:15:21.64 Stroke completed -- waiting for pressure to equalize ... @23:15:25.44 Intake.select :close @23:15:25.60 SSV.dial :exhaust @23:15:26.46 Exhaust.select :open @23:15:26.58 SS.reconfigure SSexhale @23:15:26.64 SS.seek 0ml @23:15:36.18 Exhaust.select :close @23:15:36.30 SSV.dial CBV @23:15:36.33
CBV.dial SSV @23:15:37.19 Allowing 130s for 25.0ml of remaining 650.0ml @23:15:37.20 Intake.select :open @23:15:39.38 Intake @15.5psia, vacuum from 9.0 to 13.0psi @23:15:39.39 SS.reconfigure SSinhale.with maxSpeed: 32 @23:15:39.44 SS.seek 25ml @23:16:34.58 Stroke completed -- waiting for pressure to equalize ... @23:16:37.77 Intake.select :close @23:16:37.93 SSV.dial :exhaust @23:16:38.80 Exhaust.select :open @23:16:38.92 SS.reconfigure SSexhale @23:16:38.98 SS.seek 0ml @23:16:48.66 Exhaust.select :close @23:16:48.78 SSV.dial CBV @23:16:48.81
CBV.dial SSV @23:16:49.69 Allowing 130s for 25.0ml of remaining 625.0ml @23:16:49.70 Intake.select :open @23:16:51.87 Intake @15.5psia, vacuum from 9.0 to 13.0psi @23:16:51.89 SS.reconfigure SSinhale.with maxSpeed: 32 @23:16:51.94 SS.seek 25ml @23:17:46.75 Stroke completed -- waiting for pressure to equalize ... @23:17:49.95 Intake.select :close @23:17:50.10 SSV.dial :exhaust @23:17:50.97 Exhaust.select :open @23:17:51.09 SS.reconfigure SSexhale @23:17:51.13 SS.seek 0ml @23:18:00.71 Exhaust.select :close @23:18:00.83 SSV.dial CBV @23:18:00.86
CBV.dial SSV @23:18:01.74 Allowing 130s for 25.0ml of remaining 600.0ml @23:18:01.75 Intake.select :open @23:18:03.92 Intake @15.5psia, vacuum from 9.0 to 13.0psi @23:18:03.94 SS.reconfigure SSinhale.with maxSpeed: 32 @23:18:03.99 SS.seek 25ml @23:18:58.99 Stroke completed -- waiting for pressure to equalize ... @23:19:02.70 Intake.select :close @23:19:02.85 SSV.dial :exhaust @23:19:03.72 Exhaust.select :open @23:19:03.84 SS.reconfigure SSexhale @23:19:03.89 SS.seek 0ml @23:19:13.44 Exhaust.select :close @23:19:13.56 SSV.dial CBV @23:19:13.59
CBV.dial SSV @23:19:14.46 Allowing 130s for 25.0ml of remaining 575.0ml @23:19:14.47 Intake.select :open @23:19:16.65 Intake @15.4psia, vacuum from 8.9 to 12.9psi @23:19:16.67 SS.reconfigure SSinhale.with maxSpeed: 32 @23:19:16.72 SS.seek 25ml @23:20:11.83 Stroke completed -- waiting for pressure to equalize ... @23:20:15.64 Intake.select :close @23:20:15.79 SSV.dial :exhaust @23:20:16.64 Exhaust.select :open @23:20:16.76 SS.reconfigure SSexhale @23:20:16.80 SS.seek 0ml @23:20:25.97 Exhaust.select :close @23:20:26.09 SSV.dial CBV @23:20:26.12
CBV.dial SSV @23:20:26.99 Allowing 130s for 25.0ml of remaining 550.0ml @23:20:27.00 Intake.select :open @23:20:29.17 Intake @15.4psia, vacuum from 8.9 to 12.9psi @23:20:29.19 SS.reconfigure SSinhale.with maxSpeed: 32 @23:20:29.24 SS.seek 25ml @23:21:25.00 Stroke completed -- waiting for pressure to equalize ... @23:21:28.82 Intake.select :close @23:21:28.97 SSV.dial :exhaust @23:21:29.83 Exhaust.select :open @23:21:29.95 SS.reconfigure SSexhale @23:21:29.99 SS.seek 0ml @23:21:39.75 Exhaust.select :close @23:21:39.87 SSV.dial CBV @23:21:39.90
CBV.dial SSV @23:21:40.78 Allowing 130s for 25.0ml of remaining 525.0ml @23:21:40.79 Intake.select :open @23:21:42.97 Intake @15.4psia, vacuum from 8.9 to 12.9psi @23:21:42.98 SS.reconfigure SSinhale.with maxSpeed: 32 @23:21:43.03 SS.seek 25ml @23:22:37.97 Stroke completed -- waiting for pressure to equalize ... @23:22:41.17 Intake.select :close @23:22:41.32 SSV.dial :exhaust @23:22:42.19 Exhaust.select :open @23:22:42.31 SS.reconfigure SSexhale @23:22:42.35 SS.seek 0ml @23:22:51.86 Exhaust.select :close @23:22:51.98 SSV.dial CBV @23:22:52.01
CBV.dial SSV @23:22:52.88 Allowing 130s for 25.0ml of remaining 500.0ml @23:22:52.90 Intake.select :open @23:22:55.06 Intake @15.5psia, vacuum from 9.0 to 13.0psi @23:22:55.08 SS.reconfigure SSinhale.with maxSpeed: 32 @23:22:55.13 SS.seek 25ml @23:23:50.13 Stroke completed -- waiting for pressure to equalize ... @23:23:53.31 Intake.select :close @23:23:53.47 SSV.dial :exhaust @23:23:54.33 Exhaust.select :open @23:23:54.45 SS.reconfigure SSexhale @23:23:54.50 SS.seek 0ml @23:24:03.70 Exhaust.select :close @23:24:03.82 SSV.dial CBV @23:24:04.33
CBV.dial SSV @23:24:05.21 Allowing 130s for 25.0ml of remaining 475.0ml @23:24:05.22 Intake.select :open @23:24:07.40 Intake @15.5psia, vacuum from 9.0 to 13.0psi @23:24:07.41 SS.reconfigure SSinhale.with maxSpeed: 32 @23:24:07.46 SS.seek 25ml @23:25:02.55 Stroke completed -- waiting for pressure to equalize ... @23:25:05.75 Intake.select :close @23:25:05.91 SSV.dial :exhaust @23:25:06.77 Exhaust.select :open @23:25:06.89 SS.reconfigure SSexhale @23:25:06.94 SS.seek 0ml @23:25:16.44 Exhaust.select :close @23:25:16.56 SSV.dial CBV @23:25:16.59
CBV.dial SSV @23:25:17.45 Allowing 130s for 25.0ml of remaining 450.0ml @23:25:17.46 Intake.select :open @23:25:19.64 Intake @15.4psia, vacuum from 8.9 to 12.9psi @23:25:19.65 SS.reconfigure SSinhale.with maxSpeed: 32 @23:25:19.70 SS.seek 25ml @23:26:14.81 Stroke completed -- waiting for pressure to equalize ... @23:26:17.98 Intake.select :close @23:26:18.14 SSV.dial :exhaust @23:26:19.00 Exhaust.select :open @23:26:19.12 SS.reconfigure SSexhale @23:26:19.17 SS.seek 0ml @23:26:28.36 Exhaust.select :close @23:26:28.48 SSV.dial CBV @23:26:28.51
CBV.dial SSV @23:26:29.38 Allowing 130s for 25.0ml of remaining 425.0ml @23:26:29.39 Intake.select :open @23:26:31.57 Intake @15.5psia, vacuum from 9.0 to 13.0psi @23:26:31.58 SS.reconfigure SSinhale.with maxSpeed: 32 @23:26:31.63 SS.seek 25ml @23:27:26.83 Stroke completed -- waiting for pressure to equalize ... @23:27:30.01 Intake.select :close @23:27:30.16 SSV.dial :exhaust @23:27:31.03 Exhaust.select :open @23:27:31.15 SS.reconfigure SSexhale @23:27:31.19 SS.seek 0ml @23:27:40.86 Exhaust.select :close @23:27:40.98 SSV.dial CBV @23:27:41.01
CBV.dial SSV @23:27:41.88 Allowing 130s for 25.0ml of remaining 400.0ml @23:27:41.89 Intake.select :open @23:27:44.06 Intake @15.5psia, vacuum from 9.0 to 13.0psi @23:27:44.08 SS.reconfigure SSinhale.with maxSpeed: 32 @23:27:44.13 SS.seek 25ml @23:28:38.97 Stroke completed -- waiting for pressure to equalize ... @23:28:42.14 Intake.select :close @23:28:42.30 SSV.dial :exhaust @23:28:43.17 Exhaust.select :open @23:28:43.29 SS.reconfigure SSexhale @23:28:43.33 SS.seek 0ml @23:28:53.05 Exhaust.select :close @23:28:53.17 SSV.dial CBV @23:28:53.20
CBV.dial SSV @23:28:54.08 Allowing 130s for 25.0ml of remaining 375.0ml @23:28:54.09 Intake.select :open @23:28:56.26 Intake @15.4psia, vacuum from 8.9 to 12.9psi @23:28:56.28 SS.reconfigure SSinhale.with maxSpeed: 32 @23:28:56.33 SS.seek 25ml @23:29:51.56 Stroke completed -- waiting for pressure to equalize ... @23:29:54.77 Intake.select :close @23:29:54.92 SSV.dial :exhaust @23:29:55.77 Exhaust.select :open @23:29:55.89 SS.reconfigure SSexhale @23:29:55.94 SS.seek 0ml @23:30:05.45 Exhaust.select :close @23:30:05.57 SSV.dial CBV @23:30:05.60
CBV.dial SSV @23:30:06.47 Allowing 130s for 25.0ml of remaining 350.0ml @23:30:06.49 Intake.select :open @23:30:08.66 Intake @15.4psia, vacuum from 8.9 to 12.9psi @23:30:08.68 SS.reconfigure SSinhale.with maxSpeed: 32 @23:30:08.73 SS.seek 25ml @23:31:03.77 Stroke completed -- waiting for pressure to equalize ... @23:31:06.95 Intake.select :close @23:31:07.11 SSV.dial :exhaust @23:31:07.97 Exhaust.select :open @23:31:08.09 SS.reconfigure SSexhale @23:31:08.14 SS.seek 0ml @23:31:17.81 Exhaust.select :close @23:31:17.93 SSV.dial CBV @23:31:17.96
CBV.dial SSV @23:31:18.83 Allowing 130s for 25.0ml of remaining 325.0ml @23:31:18.84 Intake.select :open @23:31:21.02 Intake @15.5psia, vacuum from 9.0 to 13.0psi @23:31:21.04 SS.reconfigure SSinhale.with maxSpeed: 32 @23:31:21.08 SS.seek 25ml @23:32:15.89 Stroke completed -- waiting for pressure to equalize ... @23:32:19.08 Intake.select :close @23:32:19.24 SSV.dial :exhaust @23:32:20.11 Exhaust.select :open @23:32:20.23 SS.reconfigure SSexhale @23:32:20.28 SS.seek 0ml @23:32:29.83 Exhaust.select :close @23:32:29.95 SSV.dial CBV @23:32:29.98
CBV.dial SSV @23:32:30.84 Allowing 130s for 25.0ml of remaining 300.0ml @23:32:30.85 Intake.select :open @23:32:33.02 Intake @15.4psia, vacuum from 8.9 to 12.9psi @23:32:33.04 SS.reconfigure SSinhale.with maxSpeed: 32 @23:32:33.09 SS.seek 25ml @23:33:28.12 Stroke completed -- waiting for pressure to equalize ... @23:33:31.29 Intake.select :close @23:33:31.45 SSV.dial :exhaust @23:33:32.30 Exhaust.select :open @23:33:32.42 SS.reconfigure SSexhale @23:33:32.47 SS.seek 0ml @23:33:42.08 Exhaust.select :close @23:33:42.20 SSV.dial CBV @23:33:42.23
CBV.dial SSV @23:33:43.11 Allowing 130s for 25.0ml of remaining 275.0ml @23:33:43.12 Intake.select :open @23:33:45.29 Intake @15.4psia, vacuum from 8.9 to 12.9psi @23:33:45.31 SS.reconfigure SSinhale.with maxSpeed: 32 @23:33:45.36 SS.seek 25ml @23:34:40.26 Stroke completed -- waiting for pressure to equalize ... @23:34:43.31 Intake.select :close @23:34:43.46 SSV.dial :exhaust @23:34:44.31 Exhaust.select :open @23:34:44.43 SS.reconfigure SSexhale @23:34:44.48 SS.seek 0ml @23:34:53.72 Exhaust.select :close @23:34:53.84 SSV.dial CBV @23:34:53.87
CBV.dial SSV @23:34:54.73 Allowing 130s for 25.0ml of remaining 250.0ml @23:34:54.74 Intake.select :open @23:34:56.92 Intake @15.4psia, vacuum from 8.9 to 12.9psi @23:34:56.94 SS.reconfigure SSinhale.with maxSpeed: 32 @23:34:56.99 SS.seek 25ml @23:35:52.15 Stroke completed -- waiting for pressure to equalize ... @23:35:55.97 Intake.select :close @23:35:56.13 SSV.dial :exhaust @23:35:57.00 Exhaust.select :open @23:35:57.12 SS.reconfigure SSexhale @23:35:57.18 SS.seek 0ml @23:36:06.80 Exhaust.select :close @23:36:06.92 SSV.dial CBV @23:36:06.95
CBV.dial SSV @23:36:07.83 Allowing 130s for 25.0ml of remaining 225.0ml @23:36:07.84 Intake.select :open @23:36:10.02 Intake @15.4psia, vacuum from 8.9 to 12.9psi @23:36:10.04 SS.reconfigure SSinhale.with maxSpeed: 32 @23:36:10.09 SS.seek 25ml @23:37:05.08 Stroke completed -- waiting for pressure to equalize ... @23:37:08.26 Intake.select :close @23:37:08.42 SSV.dial :exhaust @23:37:09.26 Exhaust.select :open @23:37:09.38 SS.reconfigure SSexhale @23:37:09.43 SS.seek 0ml @23:37:19.22 Exhaust.select :close @23:37:19.34 SSV.dial CBV @23:37:19.37
CBV.dial SSV @23:37:20.23 Allowing 130s for 25.0ml of remaining 200.0ml @23:37:20.24 Intake.select :open @23:37:22.41 Intake @15.4psia, vacuum from 8.9 to 12.9psi @23:37:22.43 SS.reconfigure SSinhale.with maxSpeed: 32 @23:37:22.48 SS.seek 25ml @23:38:17.64 Stroke completed -- waiting for pressure to equalize ... @23:38:21.46 Intake.select :close @23:38:21.61 SSV.dial :exhaust @23:38:22.48 Exhaust.select :open @23:38:22.60 SS.reconfigure SSexhale @23:38:22.66 SS.seek 0ml @23:38:31.81 Exhaust.select :close @23:38:31.93 SSV.dial CBV @23:38:31.96
CBV.dial SSV @23:38:32.83 Allowing 130s for 25.0ml of remaining 175.0ml @23:38:32.84 Intake.select :open @23:38:35.02 Intake @15.3psia, vacuum from 8.8 to 12.8psi @23:38:35.03 SS.reconfigure SSinhale.with maxSpeed: 32 @23:38:35.08 SS.seek 25ml @23:39:30.86 Stroke completed -- waiting for pressure to equalize ... @23:39:35.13 Intake.select :close @23:39:35.28 SSV.dial :exhaust @23:39:36.15 Exhaust.select :open @23:39:36.27 SS.reconfigure SSexhale @23:39:36.32 SS.seek 0ml @23:39:45.81 Exhaust.select :close @23:39:45.93 SSV.dial CBV @23:39:45.96
CBV.dial SSV @23:39:46.83 Allowing 130s for 25.0ml of remaining 150.0ml @23:39:46.84 Intake.select :open @23:39:49.02 Intake @15.4psia, vacuum from 8.9 to 12.9psi @23:39:49.04 SS.reconfigure SSinhale.with maxSpeed: 32 @23:39:49.09 SS.seek 25ml @23:40:44.23 Stroke completed -- waiting for pressure to equalize ... @23:40:47.41 Intake.select :close @23:40:47.56 SSV.dial :exhaust @23:40:48.42 Exhaust.select :open @23:40:48.54 SS.reconfigure SSexhale @23:40:48.59 SS.seek 0ml @23:40:58.42 Exhaust.select :close @23:40:58.54 SSV.dial CBV @23:40:58.57
CBV.dial SSV @23:40:59.43 Allowing 130s for 25.0ml of remaining 125.0ml @23:40:59.44 Intake.select :open @23:41:01.62 Intake @15.3psia, vacuum from 8.8 to 12.8psi @23:41:01.63 SS.reconfigure SSinhale.with maxSpeed: 32 @23:41:01.68 SS.seek 25ml @23:41:56.48 Stroke completed -- waiting for pressure to equalize ... @23:41:59.67 Intake.select :close @23:41:59.83 SSV.dial :exhaust @23:42:00.70 Exhaust.select :open @23:42:00.82 SS.reconfigure SSexhale @23:42:00.86 SS.seek 0ml @23:42:10.61 Exhaust.select :close @23:42:10.73 SSV.dial CBV @23:42:10.76
CBV.dial SSV @23:42:11.62 Allowing 130s for 25.0ml of remaining 100.0ml @23:42:11.63 Intake.select :open @23:42:13.81 Intake @15.4psia, vacuum from 8.9 to 12.9psi @23:42:13.82 SS.reconfigure SSinhale.with maxSpeed: 32 @23:42:13.87 SS.seek 25ml @23:43:09.04 Stroke completed -- waiting for pressure to equalize ... @23:43:12.86 Intake.select :close @23:43:13.01 SSV.dial :exhaust @23:43:13.86 Exhaust.select :open @23:43:13.98 SS.reconfigure SSexhale @23:43:14.03 SS.seek 0ml @23:43:23.26 Exhaust.select :close @23:43:23.38 SSV.dial CBV @23:43:23.41
CBV.dial SSV @23:43:24.28 Allowing 130s for 25.0ml of remaining 75.0ml @23:43:24.29 Intake.select :open @23:43:26.46 Intake @15.4psia, vacuum from 8.9 to 12.9psi @23:43:26.48 SS.reconfigure SSinhale.with maxSpeed: 32 @23:43:26.53 SS.seek 25ml @23:44:22.26 Stroke completed -- waiting for pressure to equalize ... @23:44:26.06 Intake.select :close @23:44:26.68 SSV.dial :exhaust @23:44:27.54 Exhaust.select :open @23:44:27.66 SS.reconfigure SSexhale @23:44:27.71 SS.seek 0ml @23:44:37.36 Exhaust.select :close @23:44:37.48 SSV.dial CBV @23:44:37.51
CBV.dial SSV @23:44:38.37 Allowing 130s for 25.0ml of remaining 50.0ml @23:44:38.38 Intake.select :open @23:44:40.56 Intake @15.4psia, vacuum from 8.9 to 12.9psi @23:44:40.58 SS.reconfigure SSinhale.with maxSpeed: 32 @23:44:40.63 SS.seek 25ml @23:45:35.67 Stroke completed -- waiting for pressure to equalize ... @23:45:38.86 Intake.select :close @23:45:39.01 SSV.dial :exhaust @23:45:39.87 Exhaust.select :open @23:45:39.99 SS.reconfigure SSexhale @23:45:40.04 SS.seek 0ml @23:45:49.59 Exhaust.select :close @23:45:49.71 SSV.dial CBV @23:45:49.74
CBV.dial SSV @23:45:50.61 Allowing 130s for 25.0ml of remaining 25.0ml @23:45:50.62 Intake.select :open @23:45:52.80 Intake @15.4psia, vacuum from 8.9 to 12.9psi @23:45:52.81 SS.reconfigure SSinhale.with maxSpeed: 32 @23:45:52.87 SS.seek 24.97ml @23:46:47.92 Stroke completed -- waiting for pressure to equalize ... @23:46:51.11 Intake.select :close @23:46:51.26 SSV.dial :exhaust @23:46:52.11 Exhaust.select :open @23:46:52.23 SS.reconfigure SSexhale @23:46:52.28 SS.seek 0ml @23:47:01.48 Exhaust.select :close @23:47:01.61 Sampled 1000.0ml @23:47:01.62 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @23:47:01.63 Intake.select :close @23:47:01.70 CSV.dial :waste @23:47:01.78 CS.seek 0ml @23:47:01.88 SSV.dial CBV,avoiding: :exhaust @23:47:01.91 CSV.dial :air @23:47:01.96
CBV.dial SSV @23:47:02.83 CS.seek 10ml @23:47:04.09
CTV.dial :air @23:47:04.61 Gate.power :sampler,:OFF @23:47:04.98
delay 15 seconds @23:47:20.01 SSV.dial :spit,avoiding: :exhaust @23:47:21.54 CTV.dial CSV,avoiding: :intake @23:47:21.58
SS.seek 0ml @23:47:21.71 SSV.dial CBV,avoiding: :exhaust @23:47:30.11 CBV.dial between SSV and waste2 @23:47:30.53 CTV.dial CSV,avoiding: :air @23:47:30.56
CSV.dial CTV @23:47:31.08 CS.reconfigure CSpressurize @23:47:31.13 CS.seek 0ml @23:47:57.35 CSV.dial :air @23:47:57.88 CS.reconfigure CSfast @23:47:57.93 CS.seek 10ml @23:48:24.46 CSV.dial between air and CTV @23:48:24.83 CS.seek 2.789ml @23:48:44.77 CTV.dial CSV,avoiding: :air @23:48:44.80
CSV.dial CTV @23:48:45.15 CS.reconfigure CSpressurize @23:48:45.20 CS.seek 0ml @23:48:50.82 BubblePoint: Intake @69.7psia, Outlet @14.5psia @23:48:50.83 CBV.dial SSV @23:48:51.21 SS.reconfigure SSconfig @23:48:51.25 SS.seek 10ml @23:48:57.09 delay 15 seconds @23:49:12.23 Evacuated: Intake @38.5psia, Outlet @13.3psia @23:49:12.24 SSV.dial :spit,avoiding: :exhaust @23:49:13.74 SS.reconfigure SSexhale @23:49:13.78 SS.seek 0ml @23:49:19.28 SS.reconfigure SSfast @23:49:19.33 SS.seek 5ml @23:49:22.11 SSV.dial CBV,avoiding: :exhaust @23:49:22.14
CBV.dial SSV @23:49:23.64 CTV.dial :air,avoiding: :intake @23:49:25.20 CSV.dial CTV @23:49:25.25
SS.seek 10ml @23:49:26.25 CSV.dial :air @23:49:26.80 CS.reconfigure CSfast @23:49:26.85 CS.seek 0ml @23:49:27.95
SSV.dial :spit,avoiding: :exhaust @23:49:29.46 SS.reconfigure SSexhale @23:49:29.50 SS.seek 0ml @23:49:30.11 CS.coast @23:49:34.59
SS.reconfigure SSfast @23:49:34.64 SS.seek 5ml @23:49:37.65 SSV.dial CBV,avoiding: :exhaust @23:49:37.68
CBV.dial SSV @23:49:40.20 SSV.dial :spit,avoiding: :exhaust @23:49:41.71 SS.reconfigure SSconfig @23:49:41.76 SS.seek 0ml @23:49:45.03 SS.coast @23:49:45.07 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @23:49:45.08 Intake.select :close @23:49:45.16 CSV.dial :waste @23:49:45.30 SSV.dial CBV,avoiding: :exhaust @23:49:45.33
CBV.dial SSV @23:49:46.08 CS.seek 0ml @23:49:46.17 CSV.dial :air @23:49:46.84
CTV.dial :air @23:49:46.86 delay 15 seconds @23:49:47.15 CS.seek 10ml @23:50:01.89
SSV.dial :spit,avoiding: :exhaust @23:50:03.40 CTV.dial CSV,avoiding: :intake @23:50:03.44
SS.reconfigure SSexhale @23:50:03.54 SS.seek 0ml @23:50:03.60 SSV.dial CBV,avoiding: :exhaust @23:50:14.11 CBV.dial between SSV and waste2 @23:50:14.55 CSV.dial between air and CTV @23:50:14.93 CS.seek 8.268ml @23:50:20.36 CTV.dial CSV,avoiding: :air @23:50:20.39
CSV.dial CTV @23:50:20.74 CS.reconfigure CSpressurize @23:50:20.79 CS.seek 0ml @23:50:42.51 CSV.dial :air @23:50:43.16 CS.reconfigure CSfast @23:50:43.21 CS.seek 10ml @23:51:09.77 CSV.dial between air and CTV @23:51:10.58 CS.seek 3.068ml @23:51:29.24 CTV.dial CSV,avoiding: :air @23:51:29.27
CSV.dial CTV @23:51:29.62 CS.reconfigure CSpressurize @23:51:29.67 CS.seek 0ml @23:51:37.35 BubblePoint: Intake @69.3psia, Outlet @14.1psia @23:51:37.36 CBV.dial SSV @23:51:37.73 SS.reconfigure SSconfig @23:51:37.77 SS.seek 10ml @23:51:43.24 delay 15 seconds @23:51:58.39 Evacuated: Intake @40.0psia, Outlet @15.2psia @23:51:58.40 SSV.dial :spit,avoiding: :exhaust @23:51:59.93 SS.reconfigure SSexhale @23:51:59.98 SS.seek 0ml @23:52:05.09 SS.reconfigure SSfast @23:52:05.14 SS.seek 5ml @23:52:07.96 SSV.dial CBV,avoiding: :exhaust @23:52:08.00
CBV.dial SSV @23:52:09.51 CTV.dial :air,avoiding: :intake @23:52:11.08 CSV.dial CTV @23:52:11.13
SS.seek 10ml @23:52:12.13 CSV.dial :air @23:52:12.68 CS.reconfigure CSfast @23:52:12.73 CS.seek 0ml @23:52:13.90
SSV.dial :spit,avoiding: :exhaust @23:52:14.61 CS.coast @23:52:15.41
SS.reconfigure SSexhale @23:52:15.46 SS.seek 0ml @23:52:20.51 SS.reconfigure SSfast @23:52:20.55 SS.seek 5ml @23:52:23.37 SSV.dial CBV,avoiding: :exhaust @23:52:23.41
CBV.dial SSV @23:52:25.93 SSV.dial :spit,avoiding: :exhaust @23:52:27.44 SS.reconfigure SSconfig @23:52:27.49 SS.seek 0ml @23:52:30.67 SS.coast @23:52:30.71 Lysing: Adding 1.5ml lysis @23:52:30.72 CBV.dial between CSV and waste1,avoiding: :DBV @23:52:32.44 CTV.dial CSV @23:52:33.98 CSV.dial CSR @23:52:34.89 Pulling 1ml of CSR.lysis @23:52:34.90 CSR.select :lysis @23:52:35.02 CS.reconfigure CSconfig @23:52:35.07 CS.seek 1ml @23:52:38.67 delay 5 seconds @23:52:43.70 CSR.select :air @23:52:43.88 CS.seek 6ml @23:52:57.64 delay 2 seconds @23:52:59.66 CSV.dial :waste1 @23:53:01.31 CS.reconfigure CSfast @23:53:01.35 CS.seek :empty @23:53:17.83 CSV.dial CSR @23:53:19.40 Pulling 1.5ml of CSR.lysis @23:53:19.41 CSR.select :lysis @23:53:19.53 CS.reconfigure CSconfig @23:53:19.58 CS.seek 1.5ml @23:53:24.48 delay 5 seconds @23:53:29.51 CSR.select :air @23:53:29.69 CS.seek 4.5ml @23:53:38.39 delay 2 seconds @23:53:40.41 CSV.dial CTV @23:53:40.93 CS.seek 1.275ml @23:53:50.18 Heating sh1 puck from tube 2 in Collection Clamp to 85C for 5:00 @23:53:50.20 CH.seek 85C,8:00 @23:58:11.35 CH.hold 5:00,85C @00:03:11.53CEST24-May-19 Collection Puck Heater at 84.4C after lysing @00:03:11.55 CS.seek 0ml @00:03:16.03 CTV.dial between spare and air,avoiding: :intake @00:03:17.22 CSV.dial :air @00:03:17.75 CS.seek 5ml @00:03:31.52 CSV.dial CTV @00:03:32.04 delay 2 seconds @00:03:34.06 CSV.dial :air @00:03:34.58 delay 2 seconds @00:03:36.60 CSV.dial CTV @00:03:37.11 delay 2 seconds @00:03:39.13 CSV.dial :air @00:03:39.66 delay 2 seconds @00:03:41.69 CS.seek 0ml @00:03:55.52 Recovering Lysate into Collection syringe @00:03:55.53 CSV.dial CBV @00:03:56.71 CBV.dial CSV @00:03:57.12 CS.seek 5ml @00:04:10.93 delay 5 seconds @00:04:15.95 CTV.dial :air @00:04:16.54 CS.seek 7ml @00:04:22.58 delay 5 seconds @00:04:27.60 CSV.dial :air2 @00:04:28.11 CS.seek 10ml @00:04:36.80 CSV.dial between air and CTV,avoiding: CBV @00:04:38.16 Lysing Complete @00:04:38.23 Storing sh1 puck in Collection Clamp to tube 1 @00:04:38.42 SC.reconfigure SCtight @00:04:38.47 SC.seek 1 @00:04:40.58 SC.reconfigure SCloose @00:04:40.80 Elbow.reconfigure TightElbow @00:04:40.85 Elbow.seek 1 @00:04:42.81 Forearm.reconfigure TightForearm @00:04:42.85 Forearm.seek 1 + 700 counts @00:04:43.91 SE.reconfigure SEpick @00:04:43.98 SE.seek beamBreak @00:04:46.94 SE.configure SEconfig @00:04:46.99 SE.seek :place @00:04:47.63 Forearm.reconfigure LooseForearm @00:04:47.67 Forearm.seek :retract @00:04:48.63 Elbow.seek CC @00:04:50.29 Elbow.reconfigure LooseElbow @00:04:50.39 Forearm.seek CC @00:04:51.45 Hand.close @00:04:52.15 CC.seek :open @00:04:56.65 Forearm.seek :retract @00:04:57.69 Elbow.reconfigure TightElbow @00:04:57.74 Elbow.seek 1 @00:05:00.28 Elbow.reconfigure LimpElbow @00:05:00.34 Forearm.seek 1 @00:05:01.36 Elbow.hold @00:05:01.44 Elbow.reconfigure TightElbow @00:05:01.49 Hand.open @00:05:02.40 Forearm.force -20 @00:05:02.45 Forearm.seek 1 + 349 counts @00:05:02.78 Forearm.reconfigure TightForearm @00:05:02.83 Forearm.seek 1 + 700 counts @00:05:03.26 SE.seek :down @00:05:04.42
Forearm.reconfigure LooseForearm @00:05:04.46 Forearm.seek :retract @00:05:06.11 SE.coast @00:05:06.15
SC.coast @00:05:06.19 Tranferring lysate to PRV @00:05:06.20 CSV.dial :air @00:05:06.56 CS.seek 10ml @00:05:06.74 CSV.dial between air and CTV @00:05:07.10 AIV.dial :bypass @00:05:07.20 Beginning samplePassC @00:05:07.21 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @00:05:07.29 Hand.open @00:05:07.66 Elbow.seek FlushPuck::Garage @00:05:09.74 Elbow.reconfigure LooseElbow @00:05:09.84 Forearm.seek :garage @00:05:10.88 Hand.close @00:05:11.79 Forearm.seek :retract @00:05:12.80 Elbow.reconfigure TightElbow @00:05:12.85 Elbow.seek CC @00:05:15.73 Elbow.reconfigure LooseElbow @00:05:15.83 Forearm.reconfigure TightForearm @00:05:15.89 Forearm.seek CC @00:05:17.21 CC.seek :closed @00:05:21.57 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.42 @00:05:21.65 Hand.open @00:05:22.47 Forearm.reconfigure LooseForearm @00:05:22.51 Forearm.seek :retract @00:05:23.55 AWV.select :air @00:05:23.56 AIV.dial :bypass @00:05:23.57 PSV.dial PSR @00:05:25.11 Pulling 1.5ml of PSR.flush PSR.select :flush @00:05:25.24 PS.seek 1.5ml @00:05:41.55 delay 5 seconds @00:05:46.58 PSV.dial :waste1 @00:05:47.11 PS.seek 0ml @00:06:03.34 PSV.dial PSR @00:06:03.87 PSR.select :air @00:06:04.50 PS.seek 2.5ml @00:06:31.06 PRV.dial CBV @00:06:31.08
CBV.dial PRV @00:06:32.27 PSV.dial :mixloop @00:06:33.41 CTV.dial :air,avoiding: :intake @00:06:33.43 PTV.dial :PRVmixing @00:06:34.27 PS.seek 0ml @00:07:00.87 PSV.dial :air @00:07:01.39 PS.seek 2.5ml @00:07:28.09 PSV.dial :mixloop @00:07:28.61 PS.seek 0ml @00:07:55.21 PSV.dial :air @00:07:55.83 PS.seek 2.5ml @00:08:22.27 PSV.dial :mixloop @00:08:22.75 PS.seek 0ml @00:08:48.99 Garaging FlushPuck in Collection Clamp @00:08:49.07 Elbow.reconfigure TightElbow @00:08:49.12 Elbow.seek CC @00:08:49.26 Elbow.reconfigure LooseElbow @00:08:49.36 Forearm.seek CC @00:08:50.43 Hand.close @00:08:51.16 CC.seek :open @00:08:55.56 Forearm.seek :retract @00:08:56.63 Elbow.reconfigure TightElbow @00:08:56.68 Elbow.seek :garage @00:08:59.95 Elbow.reconfigure LooseElbow @00:09:00.05 Forearm.reconfigure TightForearm @00:09:00.09 Forearm.seek :garage @00:09:01.27 Hand.open @00:09:02.09 Forearm.reconfigure LooseForearm @00:09:02.14 Forearm.seek :retract @00:09:03.19 Connecting PS to CS to transfer sample @00:09:03.20 CBV.dial AIV @00:09:03.22 AIV.dial :bypass @00:09:03.23 PSV.dial :mixloop @00:09:03.24 PTV.dial :PRVmixing @00:09:03.25 PRV.dial AIV @00:09:03.27 CTV.dial :air,avoiding: :intake @00:09:03.28 Loading lyfil puck from tube 2 into Collection Clamp @00:09:03.53 SC.reconfigure SCtight @00:09:03.58 SC.seek 2 @00:09:05.75 SC.reconfigure SCloose @00:09:05.97 Elbow.reconfigure TightElbow @00:09:06.02 Elbow.seek 2 @00:09:08.18 Forearm.reconfigure TightForearm @00:09:08.23 Forearm.seek 2 + 700 counts @00:09:09.32 SE.reconfigure SEpick @00:09:09.40 SE.seek beamBreak @00:09:10.60 SE.configure SEconfig @00:09:10.76 Elbow.seek 2 @00:09:10.96 Forearm.seek 2 + 300 counts @00:09:11.19 Forearm.reconfigure LooseForearm @00:09:11.23 Hand.close @00:09:12.04 SE.seek :down @00:09:12.27
Forearm.seek :retract @00:09:13.01 SE.coast @00:09:13.21
Elbow.seek CC @00:09:15.37 Elbow.reconfigure LooseElbow @00:09:15.47 Forearm.reconfigure TightForearm @00:09:15.52 Forearm.seek CC @00:09:16.85 CC.seek :closed @00:09:21.17 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.40 @00:09:21.18 Hand.open @00:09:21.97 Forearm.reconfigure LooseForearm @00:09:22.01 Forearm.seek :retract @00:09:23.07 SC.coast @00:09:23.11 CTV.dial CSV,avoiding: :intake @00:09:24.68 CSV.dial CTV @00:09:25.02 CS.seek 5ml @00:09:38.93 delay 1:20 @00:10:58.96 CS.seek 2.5ml @00:11:06.31 delay 40 seconds @00:11:46.35 CS.seek 0ml @00:11:54.09 delay 1:00 @00:12:54.12 PSV.dial :air @00:12:54.63 PS.seek 2.5ml @00:13:20.99 PSV.dial :mixloop @00:13:21.49 delay 3 seconds @00:13:24.51 PSV.dial :air @00:13:25.01 delay 2 seconds @00:13:27.03 PSV.dial :mixloop @00:13:27.54 delay 3 seconds @00:13:30.56 PSV.dial :waste1 @00:13:32.08 PS.seek :empty @00:13:58.21 CBV.dial :waste1 @00:13:58.73 delay 5 seconds @00:14:03.76 CBV.dial AIV @00:14:04.28 delay 10 seconds @00:14:14.31 CBV.dial :waste1 @00:14:14.82 delay 10 seconds @00:14:24.85 CBV.dial AIV @00:14:25.37 delay 10 seconds @00:14:35.40 CTV.dial CSV,avoiding: :intake @00:14:35.41 CBV.dial between AIV and waste1 @00:14:35.78 CSV.dial :air @00:14:36.32 CS.seek 10ml @00:15:02.90 CSV.dial CTV @00:15:03.43 CS.seek 0ml @00:15:30.37 CBV.dial :waste1 @00:15:30.72 delay 10 seconds @00:15:40.75 CTV.dial :air,avoiding: :intake @00:15:42.38 Storing lyfil puck in Collection Clamp to tube 1 @00:15:42.56 SC.reconfigure SCtight @00:15:42.61 SC.seek 1 @00:15:44.73 SC.reconfigure SCloose @00:15:44.95 Elbow.reconfigure TightElbow @00:15:45.00 Elbow.seek 1 @00:15:46.91 Forearm.reconfigure TightForearm @00:15:46.96 Forearm.seek 1 + 700 counts @00:15:47.99 SE.reconfigure SEpick @00:15:48.07 SE.seek beamBreak @00:15:51.27 SE.configure SEconfig @00:15:51.43 Elbow.reconfigure LimpElbow @00:15:51.48 Forearm.reconfigure LooseForearm @00:15:51.52 Forearm.seek 1 @00:15:51.84 Elbow.hold @00:15:51.91 Elbow.reconfigure TightElbow @00:15:52.52 SE.seek 0.06 Pucks below Top Plate @00:15:52.97 Forearm.force -20 @00:15:53.01 Forearm.seek 1 + 342 counts @00:15:53.34 Forearm.reconfigure TightForearm @00:15:53.39 Forearm.seek 1 + 700 counts @00:15:53.71 SE.seek 1.06 Pucks below Top Plate @00:15:54.43 Forearm.reconfigure LooseForearm @00:15:54.48 Forearm.seek :retract @00:15:55.43 Elbow.seek CC @00:15:57.12 Elbow.reconfigure LooseElbow @00:15:57.22 Forearm.seek CC @00:15:58.29 Hand.close @00:15:59.04 CC.seek :open @00:16:03.56 Forearm.seek :retract @00:16:04.64 Elbow.reconfigure TightElbow @00:16:04.69 Elbow.seek 1 @00:16:06.58 Elbow.reconfigure LimpElbow @00:16:06.63 Forearm.seek 1 @00:16:07.64 Elbow.hold @00:16:07.72 Elbow.reconfigure TightElbow @00:16:07.77 Hand.open @00:16:08.67 Forearm.force -20 @00:16:08.71 Forearm.seek 1 + 349 counts @00:16:09.04 Forearm.reconfigure TightForearm @00:16:09.09 Forearm.seek 1 + 700 counts @00:16:09.37 SE.seek :down @00:16:10.51
Forearm.reconfigure LooseForearm @00:16:10.56 Forearm.seek :retract @00:16:12.13 SE.coast @00:16:12.17
SC.coast @00:16:12.20 PSV.dial :air @00:16:13.43 PS.seek 0.499ml @00:16:19.31 delay 3 seconds @00:16:22.33 PSV.dial :mixloop @00:16:22.81 PRV.dial :air @00:16:23.32 delay 3 seconds @00:16:26.34 PRV.dial CBV @00:16:26.84 CBV.dial AIV @00:16:27.37 PRV.dial between CBV and air @00:16:27.73 PRV.dial :air @00:16:28.14 PS.reconfigure PSslow @00:16:28.19 PS.seek 0.599ml @00:16:30.68 PRV.dial :waste2 @00:16:31.51 PS.reconfigure PSconfig @00:16:31.55 PS.seek :empty @00:16:38.43 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @00:16:38.51 Hand.open @00:16:38.91 Elbow.seek FlushPuck::Garage @00:16:40.91 Elbow.reconfigure LooseElbow @00:16:41.01 Forearm.seek :garage @00:16:42.06 Hand.close @00:16:43.42 Forearm.seek :retract @00:16:44.49 Elbow.reconfigure TightElbow @00:16:44.53 Elbow.seek CC @00:16:47.41 Elbow.reconfigure LooseElbow @00:16:47.52 Forearm.reconfigure TightForearm @00:16:47.56 Forearm.seek CC @00:16:48.96 CC.seek :closed @00:16:53.21 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.38 @00:16:53.28 Hand.open @00:16:54.11 Forearm.reconfigure LooseForearm @00:16:54.16 Forearm.seek :retract @00:16:55.24 Clearing PML for modified lysate @00:16:55.25 CTV.dial :air,avoiding: :intake @00:16:55.26 PRV.dial :waste2 @00:16:55.28 PTV.dial :PRV,avoiding: :puck @00:16:56.12 delay 2 seconds @00:16:58.14 PTV.dial :PRVmixing,avoiding: :puck @00:16:58.98 delay 2 seconds @00:17:01.00 PSV.dial :PML @00:17:01.02 delay 2 seconds @00:17:03.04 PRV.dial :air @00:17:03.87 delay 2 seconds @00:17:05.95 PS.seek 1.499ml @00:17:22.09 delay 5 seconds @00:17:27.13 PS.seek 2.5ml @00:17:38.15 PSV.dial :air @00:17:38.65 PS.seek 0ml @00:18:04.96 mixing lysate with speDil @00:18:04.97 PSV.dial :PML @00:18:05.59 CBV.dial :PRV @00:18:05.60 PRV.dial :air @00:18:05.62 delay 2 seconds @00:18:07.64 PRV.dial :speDil @00:18:08.17 delay 2 seconds @00:18:10.26 PS.reconfigure PSslow1 @00:18:10.32 PS.seek 0.225ml @00:18:34.06 delay 5 seconds @00:18:39.09 PRV.dial CBV @00:18:39.95 delay 2 seconds @00:18:42.04 PS.seek 0.475ml @00:19:07.75 delay 5 seconds @00:19:12.77 PRV.dial :air @00:19:13.29 delay 2 seconds @00:19:15.32 PS.seek 0.965ml @00:20:05.68 delay 10 seconds @00:20:15.71 PRV.dial CBV @00:20:16.23 CTV.dial :air,avoiding: :intake @00:20:16.24 PSV.dial :air @00:20:16.74 PS.reconfigure PSconfig @00:20:16.79 PS.seek 2.5ml @00:20:33.15 delay 3 seconds @00:20:36.17 PSV.dial PTV @00:20:37.14 PTV.dial :PRV,avoiding: :puck @00:20:37.98 PS.seek 0ml @00:21:04.37 PSV.dial :air @00:21:05.18 PS.seek 2.5ml @00:21:31.34 delay 3 seconds @00:21:34.36 PSV.dial PTV @00:21:35.31 PTV.dial :PRV,avoiding: :puck @00:21:35.33 PS.seek 0ml @00:22:01.50 Pushing modified lysate into AIV Injection Loop @00:22:01.51 CBV.dial AIV @00:22:01.53 AIV.dial :bypass @00:22:01.54 PRV.dial CBV @00:22:02.02 PSV.dial :air @00:22:02.87 PS.seek 2.5ml @00:22:29.00 delay 3 seconds @00:22:32.02 PSV.dial :PML @00:22:32.64 PTV.dial :PRVmixing,avoiding: :puck @00:22:33.55 PS.reconfigure PSslow1 @00:22:33.60 PS.seek 1.74ml @00:23:51.00 delay 5 seconds @00:23:56.03 AIV.dial :sample @00:23:56.72 AWV.select :waste @00:23:56.89 PS.reconfigure PSslow2 @00:23:56.94 PS.seek 1.22ml @00:25:44.40 delay 15 seconds @00:25:59.43 AIV.dial :bypass @00:26:00.09 PS.reconfigure PSconfig @00:26:00.14 PS.seek 0ml @00:26:13.50 PSV.dial :air @00:26:13.98 PS.seek 2.5ml @00:26:40.31 PSV.dial :PML @00:26:40.80 PS.seek 0ml @00:27:07.09 AIV.dial :coilToMFB @00:27:07.10 AWV.select :air @00:27:07.27 Extracting Nucleic Acid from AIV @00:27:07.30
Beginning samplePassD @00:27:07.33 SPE.hold 2:46:40,55C @00:27:07.44 ASV.dial :SPEdelivery @00:27:07.51
AWV.select :air @00:27:07.52 AIV.dial :bypass @00:27:07.53 PSV.dial PSR @00:27:07.87 delay 20 seconds @00:27:08.83
Pulling 1.5ml of PSR.killMFB @00:27:08.84 PSR.select :killMFB @00:27:08.97 PS.seek 1.5ml @00:27:25.88 delay 5 seconds @00:27:27.90 primeLysateINT @00:27:27.91 AWV.select :air @00:27:27.93 AIV.dial :coilToMFB @00:27:27.94 ATV.dial AIV @00:27:29.92 AS.reconfigure ASslow1 @00:27:29.97 AS.seek 0.299ml @00:27:30.90
PSV.dial :waste1 @00:27:31.45 PS.seek 0ml @00:27:48.59 PSV.dial PSR @00:27:49.12 PSR.select :air @00:27:49.24 PS.seek 2.5ml @00:28:17.31 PRV.dial CBV @00:28:17.34
CBV.dial PRV @00:28:17.36 PSV.dial :mixloop @00:28:18.56 CTV.dial :air,avoiding: :intake @00:28:18.58 PTV.dial :PRVmixing @00:28:18.60 PS.seek 0ml @00:28:30.65 delay 10 seconds @00:28:40.68 ATV.dial :air2 @00:28:41.61 AS.seek 0.329ml @00:28:46.60
PSV.dial PSR @00:28:47.88 Pulling 1.5ml of PSR.flush PSR.select :flush @00:28:47.96 ATV.dial :waste2 @00:28:48.03
PS.seek 1.5ml @00:28:48.58 AS.reconfigure ASnorm @00:28:48.63 AS.seek :empty @00:29:04.98
delay 5 seconds @00:29:05.62 Lysate primed! @00:29:05.63 AFV.select :holdingCoil @00:29:05.64 ATV.dial :air2 @00:29:06.33 AS.reconfigure ASslow1 @00:29:06.38 AS.seek 0.069ml @00:29:09.99
PSV.dial :waste1 @00:29:10.52 PS.seek 0ml @00:29:20.77 delay 3 seconds @00:29:23.80 ATV.dial :AIV @00:29:24.74 AS.seek 0.469ml @00:29:27.41
PSV.dial PSR @00:29:27.95 PSR.select :air @00:29:28.08 PS.seek 2.5ml @00:29:55.75 PRV.dial CBV @00:29:55.78
CBV.dial PRV @00:29:55.80 PSV.dial :mixloop @00:29:57.50 CTV.dial :air,avoiding: :intake @00:29:57.52 PTV.dial :PRVmixing @00:29:57.54 PS.seek 0ml @00:30:25.00 PSV.dial PSR @00:30:26.29 Pulling 1.5ml of PSR.flush @00:30:26.30 PSR.select :flush @00:30:26.43 PS.seek 1.5ml @00:30:43.01 delay 5 seconds @00:30:45.43 delay 5 seconds @00:30:48.03
PSV.dial :waste1 @00:30:48.56 PS.seek 0ml @00:30:50.50 Solid Phase Extraction Heater at 49.2C @00:30:50.52 ATV.dial ASV @00:30:50.95 ASV.dial :SPEdelivery @00:30:51.04 AS.seek 0.041ml @00:31:05.12
PSV.dial PSR @00:31:05.64 PSR.select :air @00:31:05.77 PS.seek 2.5ml @00:31:32.94 PRV.dial CBV @00:31:32.97
CBV.dial PRV @00:31:32.99 PSV.dial :mixloop @00:31:34.17 CTV.dial :air,avoiding: :intake @00:31:34.19 PTV.dial :PRVmixing @00:31:34.21 PS.seek 0ml @00:32:01.16 PSV.dial PSR @00:32:02.41 Pulling 1.5ml of PSR.flush @00:32:02.42 PSR.select :flush @00:32:02.55 PS.seek 1.5ml @00:32:17.40 delay 10 seconds @00:32:18.84
delay 5 seconds @00:32:23.87 PSV.dial :waste1 @00:32:24.39 PS.seek 0ml @00:32:27.42 rinseLysateLineINT @00:32:27.44 AFV.select :carrierSolution @00:32:27.54 delay 2 seconds @00:32:29.58 AS.reconfigure ASconfig @00:32:29.63 AS.seek 1ml @00:32:40.78
PSV.dial PSR @00:32:41.30 PSR.select :air @00:32:41.42 PS.seek 2.5ml @00:33:08.31 PRV.dial CBV @00:33:08.34
CBV.dial PRV @00:33:08.36 PSV.dial :mixloop @00:33:09.53 CTV.dial :air,avoiding: :intake @00:33:09.54 PTV.dial :PRVmixing @00:33:09.57 PS.reconfigure PSslow @00:33:09.62 PS.seek 1.2ml @00:33:25.04 delay 1 second @00:33:26.06 AFV.select :holdingCoil @00:33:26.16 delay 1 second @00:33:27.19 ATV.dial AIV @00:33:27.76 AIV.dial :coilToMFB @00:33:27.78 AWV.select :waste @00:33:27.89 AS.reconfigure ASslow0 @00:33:27.94 AS.seek 0ml @00:33:36.41
PSV.dial :waste1 @00:33:37.93 PS.reconfigure PSconfig @00:33:37.98 PS.seek 0ml @00:33:51.22 PSV.dial :air @00:33:52.51 PS.seek 2.5ml @00:34:19.16 PSV.dial :mixloop @00:34:19.70 PS.reconfigure PSslow @00:34:19.75 PS.seek 0ml @00:35:08.85 ATV.dial :air2 @00:35:09.56 AS.reconfigure ASslow1 @00:35:09.61 AS.seek 0.05ml @00:35:10.91
PSV.dial :air @00:35:11.56 PS.reconfigure PSconfig @00:35:11.61 PS.seek 2.5ml @00:35:19.99 ATV.dial :AIV @00:35:20.85 AS.seek 0.01ml @00:35:29.10 ATV.dial :waste @00:35:30.85 AS.seek :empty @00:35:33.29 ethanol wash @00:35:33.30 ATV.dial :air2 @00:35:34.64 AS.reconfigure ASslow2 @00:35:34.69 AS.seek 0.009ml @00:35:38.31
PSV.dial :mixloop @00:35:38.79 PS.reconfigure PSslow @00:35:39.36 PS.seek 0ml @00:35:45.28 delay 3 seconds @00:35:48.31 Pulling 0.4ml of Ethanol Wash @00:35:48.32 ATV.dial :ethanol @00:35:49.19 AS.reconfigure ASslow1 @00:35:49.24 AS.seek 0.409ml @00:36:30.50
Cleaning Collection Syringe @00:36:30.51 CSV.dial CSR @00:36:31.09 Pulling 4ml of CSR.flush @00:36:31.10 CSR.select :flush @00:36:31.23 CS.seek 4ml @00:36:42.61 delay 5 seconds @00:36:47.63 CSR.select :air @00:36:47.76 CS.seek 10ml @00:37:04.29 CSV.dial CTV @00:37:04.33
CTV.dial CSV @00:37:05.98 CBV.dial :waste1 @00:37:06.53 CS.seek 0ml @00:37:10.01 delay 3 seconds @00:37:13.04 ATV.dial :air1 @00:37:13.46 delay 3 seconds @00:37:16.55 AS.seek 0.434ml @00:37:21.87 delay 3 seconds @00:37:24.89 ATV.dial :ethanol @00:37:25.53 AS.seek 0.419ml @00:37:28.81 delay 3 seconds @00:37:31.83 ATV.dial ASV @00:37:33.25 AS.seek 0.092ml @00:37:33.57
CSV.dial CSR @00:37:34.17 Pulling 4ml of CSR.flush @00:37:34.18 CSR.select :flush @00:37:34.31 CS.seek 4ml @00:37:45.70 delay 5 seconds @00:37:50.73 CSR.select :air @00:37:50.86 CS.seek 10ml @00:38:07.42 CSV.dial CTV @00:38:07.46
CTV.dial CSV @00:38:07.99 CBV.dial :waste1 @00:38:08.01 CS.seek 0ml @00:38:34.80 Garaging FlushPuck in Collection Clamp @00:38:34.87 Elbow.reconfigure TightElbow @00:38:34.92 Elbow.seek CC @00:38:35.19 Elbow.reconfigure LooseElbow @00:38:35.30 Forearm.seek CC @00:38:36.35 Hand.close @00:38:37.06 CC.seek :open @00:38:39.28 delay 3 seconds @00:38:41.45
Forearm.seek :retract @00:38:42.30 ATV.dial :waste2 @00:38:42.48
Elbow.reconfigure TightElbow @00:38:42.53 Elbow.seek :garage @00:38:43.10 AS.reconfigure ASconfig @00:38:43.15 AS.seek :empty @00:38:45.80
Elbow.reconfigure LooseElbow @00:38:45.90 Forearm.reconfigure TightForearm @00:38:45.95 Forearm.seek :garage @00:38:47.12 Hand.open @00:38:47.87 Forearm.reconfigure LooseForearm @00:38:47.92 Forearm.seek :retract @00:38:48.79 ATV.dial :air2 @00:38:48.98 Whole Cell Archive sampling 1000ml @00:38:49.00 Loading wcr puck from tube 2 into Collection Clamp @00:38:49.24 AS.reconfigure ASslow1 @00:38:49.33 AS.seek 1ml @00:38:49.37 SC.reconfigure SCtight @00:38:49.45 SC.seek 2 @00:38:51.54 SC.reconfigure SCloose @00:38:52.27 Elbow.reconfigure TightElbow @00:38:52.35 Elbow.seek 2 @00:38:54.59 Forearm.reconfigure TightForearm @00:38:54.65 Forearm.seek 2 + 700 counts @00:38:55.76 SE.reconfigure SEpick @00:38:55.84 SE.seek beamBreak @00:38:57.19 SE.configure SEconfig @00:38:57.37 Elbow.seek 2 @00:38:57.59 Forearm.seek 2 + 300 counts @00:38:57.98 Forearm.reconfigure LooseForearm @00:38:58.03 Hand.close @00:38:58.83 SE.seek :down @00:38:59.07 Forearm.seek :retract @00:38:59.90 SE.coast @00:39:00.07 Elbow.seek CC @00:39:01.78 Elbow.reconfigure LooseElbow @00:39:01.89 Forearm.reconfigure TightForearm @00:39:01.94 Forearm.seek CC @00:39:03.34 CC.seek :closed @00:39:07.70 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.39 @00:39:07.72 Hand.open @00:39:08.51 Forearm.reconfigure LooseForearm @00:39:08.56 Forearm.seek :retract @00:39:09.62 SC.coast @00:39:09.66 collectSample 1000ml w/bubble point of 55psi @00:39:09.68 Priming 7ml @00:39:09.69 Gate.power :sampler,:ON @00:39:09.70 Intake.select :close @00:39:09.71 Exhaust.select :close @00:39:09.73 SS.hold @00:39:09.96 Calibrating pressure sensors @00:39:09.97 CTV.dial :air @00:39:11.53 SSV.dial :spit,avoiding: :exhaust @00:39:11.56 SS.reconfigure SSfast @00:39:11.61 SS.seek 5ml @00:39:14.56 SSV.dial CBV @00:39:14.60 CBV.dial SSV @00:39:16.20 delay 3.8 seconds @00:39:20.15 SSV.dial :spit @00:39:21.66 SS.seek 0ml @00:39:24.45 SSV.dial CBV @00:39:25.97 CTV.dial :intake @00:39:26.85 Waiting for external sampler to finish priming... @00:39:26.87 delayUntil "00:44:09.70" @00:42:10.93 delay 5 seconds @00:42:15.96 ATV.dial ASV @00:42:16.99 ASV.dial :SPEdelivery @00:42:17.01 AS.seek 0.02ml @00:44:09.72 SSV.dial CBV @00:44:09.76 CBV.dial SSV @00:44:09.77 Allowing 40s for 7.0ml of remaining 7.0ml @00:44:09.78 Intake.select :open @00:44:11.96 Intake @15.4psia, vacuum from 8.9 to 12.9psi @00:44:11.98 SS.reconfigure SSinhale.with maxSpeed: 150 @00:44:12.56 SS.seek 7ml @00:44:18.51 Stroke completed -- waiting for pressure to equalize ... @00:44:33.08 Intake.select :close @00:44:33.23 SSV.dial :exhaust @00:44:34.10 Exhaust.select :open @00:44:34.22 SS.reconfigure SSexhale @00:44:34.27 SS.seek 0ml @00:44:38.54 Exhaust.select :close @00:44:38.67 Sampling 1000.0ml @00:44:38.70 Intake.select :close @00:44:38.71 Exhaust.select :close @00:44:38.72 SS.reconfigure SSconfig @00:44:38.76 SS.hold @00:44:38.99 CTV.dial :intake @00:44:39.01 SSV.dial CBV @00:44:39.04 CBV.dial SSV @00:44:39.92 Allowing 130s for 25.0ml of remaining 1000.0ml @00:44:39.94 Intake.select :open @00:44:42.12 Intake @15.5psia, vacuum from 9.0 to 13.0psi @00:44:42.13 SS.reconfigure SSinhale.with maxSpeed: 150 @00:44:42.18 SS.seek 25ml @00:44:47.37 Vacuum too high -- waiting up to 40s @00:44:58.38 Intake @15.6psia, vacuum from 9.1 to 13.1psi @00:44:58.40 SS.reconfigure SSinhale.with maxSpeed: 135 @00:44:58.45 SS.seek 25ml @00:45:02.97 Vacuum too high -- waiting up to 40s @00:45:13.42 Intake @15.6psia, vacuum from 9.1 to 13.1psi @00:45:13.44 SS.reconfigure SSinhale.with maxSpeed: 122 @00:45:13.49 SS.seek 25ml @00:45:17.72 Vacuum too high -- waiting up to 40s @00:45:27.87 Intake @15.6psia, vacuum from 9.1 to 13.1psi @00:45:27.88 SS.reconfigure SSinhale.with maxSpeed: 110 @00:45:27.93 SS.seek 25ml @00:45:32.45 Stroke completed -- waiting for pressure to equalize ... @00:45:34.38 delay 5 seconds @00:45:39.40 ATV.dial :air2 @00:45:40.58 AS.seek 0.52ml @00:45:45.80 Intake.select :close @00:45:45.96 SSV.dial :exhaust @00:45:46.84 Exhaust.select :open @00:45:46.96 SS.reconfigure SSexhale @00:45:47.01 SS.seek 0ml @00:45:56.48 Exhaust.select :close @00:45:56.60 SSV.dial CBV @00:45:56.63 CBV.dial SSV @00:45:57.53 Allowing 130s for 25.0ml of remaining 975.0ml @00:45:57.55 Intake.select :open @00:45:59.73 Intake @15.5psia, vacuum from 9.0 to 13.0psi @00:45:59.75 SS.reconfigure SSinhale.with maxSpeed: 110 @00:45:59.80 SS.seek 25ml @00:46:03.97 Vacuum too high -- waiting up to 40s @00:46:10.10 SS.reconfigure SSinhale.with maxSpeed: 99 @00:46:10.15 SS.seek 25ml @00:46:14.04 Vacuum too high -- waiting up to 40s @00:46:20.74 Intake @15.5psia, vacuum from 9.0 to 13.0psi @00:46:20.76 SS.reconfigure SSinhale.with maxSpeed: 89 @00:46:20.81 SS.seek 25ml @00:46:24.97 Vacuum too high -- waiting up to 40s @00:46:31.60 Intake @15.5psia, vacuum from 9.0 to 13.0psi @00:46:31.61 SS.reconfigure SSinhale.with maxSpeed: 80 @00:46:31.66 SS.seek 25ml @00:46:36.94 Vacuum too high -- waiting up to 40s @00:46:44.42 Intake @15.5psia, vacuum from 9.0 to 13.0psi @00:46:44.43 SS.reconfigure SSinhale.with maxSpeed: 72 @00:46:44.49 SS.seek 25ml @00:46:50.90 Stroke completed -- waiting for pressure to equalize ... @00:47:01.68 Intake.select :close @00:47:01.83 SSV.dial :exhaust @00:47:02.70 Exhaust.select :open @00:47:02.82 SS.reconfigure SSexhale @00:47:02.87 SS.seek 0ml @00:47:12.45 Exhaust.select :close @00:47:12.57 SSV.dial CBV @00:47:12.60 CBV.dial SSV @00:47:13.48 Allowing 130s for 25.0ml of remaining 950.0ml @00:47:13.50 Intake.select :open @00:47:15.69 Intake @15.5psia, vacuum from 9.0 to 13.0psi @00:47:15.71 SS.reconfigure SSinhale.with maxSpeed: 72 @00:47:15.76 SS.seek 25ml @00:47:21.06 Vacuum too high -- waiting up to 40s @00:47:21.36 delay 5 seconds @00:47:25.97 Intake @15.4psia, vacuum from 8.9 to 12.9psi @00:47:25.99 SS.reconfigure SSinhale.with maxSpeed: 65 @00:47:26.04 SS.seek 25ml @00:47:26.39 ATV.dial ASV @00:47:27.43 ASV.dial :wasteR @00:47:27.91 AS.seek 0.3ml @00:47:32.56 Vacuum too high -- waiting up to 40s @00:47:38.94 Intake @15.5psia, vacuum from 9.0 to 13.0psi @00:47:38.96 SS.reconfigure SSinhale.with maxSpeed: 59 @00:47:39.01 SS.seek 25ml @00:47:46.47 Vacuum too high -- waiting up to 40s @00:47:53.13 Intake @15.4psia, vacuum from 8.9 to 12.9psi @00:47:53.15 SS.reconfigure SSinhale.with maxSpeed: 53 @00:47:53.20 SS.seek 25ml @00:48:04.99 Stroke completed -- waiting for pressure to equalize ... @00:48:12.35 delay 20 seconds @00:48:16.48 Intake.select :close @00:48:16.64 SSV.dial :exhaust @00:48:17.51 Exhaust.select :open @00:48:17.63 SS.reconfigure SSexhale @00:48:17.68 SS.seek 0ml @00:48:26.84 Exhaust.select :close @00:48:26.96 SSV.dial CBV @00:48:26.99 CBV.dial SSV @00:48:27.89 Allowing 130s for 25.0ml of remaining 925.0ml @00:48:27.91 Intake.select :open @00:48:30.09 Intake @15.4psia, vacuum from 8.9 to 12.9psi @00:48:30.11 SS.reconfigure SSinhale.with maxSpeed: 53 @00:48:30.16 SS.seek 25ml @00:48:32.38 AS.seek 0.07ml @00:48:40.78 Vacuum too high -- waiting up to 40s @00:48:46.37 Intake @15.4psia, vacuum from 8.9 to 12.9psi @00:48:46.39 SS.reconfigure SSinhale.with maxSpeed: 48 @00:48:46.44 SS.seek 25ml @00:49:03.31 Vacuum too high -- waiting up to 40s @00:49:11.52 Intake @15.4psia, vacuum from 8.9 to 12.9psi @00:49:11.54 SS.reconfigure SSinhale.with maxSpeed: 43 @00:49:11.59 SS.seek 25ml @00:49:18.88 delay 20 seconds @00:49:22.96 Stroke completed -- waiting for pressure to equalize ... @00:49:31.21 Intake.select :close @00:49:31.36 SSV.dial :exhaust @00:49:32.23 Exhaust.select :open @00:49:32.35 SS.reconfigure SSexhale @00:49:32.40 SS.seek 0ml @00:49:38.91 ATV.dial :waste2 @00:49:39.71 AS.reconfigure ASconfig @00:49:39.75 AS.seek :empty @00:49:42.06 Exhaust.select :close @00:49:42.18 SSV.dial CBV @00:49:42.21 CBV.dial SSV @00:49:43.10 Allowing 130s for 25.0ml of remaining 900.0ml @00:49:43.12 Intake.select :open @00:49:44.13 Elution @00:49:44.14 ATV.dial :air1 @00:49:45.33 Intake @15.5psia, vacuum from 9.0 to 13.0psi @00:49:45.37 SS.reconfigure SSinhale.with maxSpeed: 43 @00:49:45.48 SS.seek 25ml @00:49:45.51 AS.reconfigure ASslow1 @00:49:45.61 AS.seek 0.049ml @00:49:56.62 delay 3 seconds @00:49:59.65 ATV.dial :ElutionWater @00:50:00.38 AS.seek 0.109ml @00:50:03.87 Vacuum too high -- waiting up to 40s @00:50:09.56 Intake @15.2psia, vacuum from 8.7 to 12.7psi @00:50:09.58 SS.reconfigure SSinhale.with maxSpeed: 39 @00:50:09.63 SS.seek 25ml @00:50:12.74 delay 3 seconds @00:50:15.77 ATV.dial ASV,via: :air2 @00:50:18.01 ASV.dial :SPEelution @00:50:18.63 AFV.select :carrierSolution @00:50:18.80 AS.reconfigure ASnorm @00:50:18.85 AS.seek 0.759ml @00:50:34.98 Stroke completed -- waiting for pressure to equalize ... @00:50:45.12 Intake.select :close @00:50:45.28 SSV.dial :exhaust @00:50:46.15 Exhaust.select :open @00:50:46.27 SS.reconfigure SSexhale @00:50:46.32 SS.seek 0ml @00:50:51.88 delay 3 seconds @00:50:54.91 AFV.select :holdingCoil @00:50:55.07 AS.reconfigure ASslow1 @00:50:55.13 AS.seek 0.474ml @00:50:55.88 Exhaust.select :close @00:50:56.00 SSV.dial CBV @00:50:56.03 CBV.dial SSV @00:50:56.89 Allowing 130s for 25.0ml of remaining 875.0ml @00:50:56.91 Intake.select :open @00:50:59.09 Intake @15.5psia, vacuum from 9.0 to 13.0psi @00:50:59.11 SS.reconfigure SSinhale.with maxSpeed: 39 @00:50:59.16 SS.seek 25ml @00:51:40.37 Vacuum too high -- waiting up to 40s @00:51:48.33 Intake @15.4psia, vacuum from 8.9 to 12.9psi @00:51:48.34 SS.reconfigure SSinhale.with maxSpeed: 35 @00:51:48.39 SS.seek 25ml @00:51:52.70 delay 5 seconds @00:51:53.77 Stroke completed -- waiting for pressure to equalize ... @00:51:57.79 AS.reconfigure ASslow2 @00:51:57.84 AS.seek 0.439ml @00:51:58.84 Intake.select :close @00:51:58.99 SSV.dial :exhaust @00:51:59.84 Exhaust.select :open @00:51:59.96 SS.reconfigure SSexhale @00:52:00.01 SS.seek 0ml @00:52:09.60 Exhaust.select :close @00:52:09.72 SSV.dial CBV @00:52:09.75 CBV.dial SSV @00:52:10.62 Allowing 130s for 25.0ml of remaining 850.0ml @00:52:10.64 Intake.select :open @00:52:12.81 Intake @15.5psia, vacuum from 9.0 to 13.0psi @00:52:12.83 SS.reconfigure SSinhale.with maxSpeed: 35 @00:52:12.88 SS.seek 25ml @00:52:33.21 delay 1:00 @00:53:03.12 Stroke completed -- waiting for pressure to equalize ... @00:53:12.60 Intake.select :close @00:53:12.75 SSV.dial :exhaust @00:53:13.62 Exhaust.select :open @00:53:13.75 SS.reconfigure SSexhale @00:53:13.80 SS.seek 0ml @00:53:23.43 Exhaust.select :close @00:53:23.55 SSV.dial CBV @00:53:23.58 CBV.dial SSV @00:53:24.48 Allowing 130s for 25.0ml of remaining 825.0ml @00:53:24.50 Intake.select :open @00:53:26.68 Intake @15.5psia, vacuum from 9.0 to 13.0psi @00:53:26.70 SS.reconfigure SSinhale.with maxSpeed: 35 @00:53:26.75 SS.seek 25ml @00:53:33.24 pushing eluate to holding coil @00:53:33.32 AS.seek 0.279ml @00:54:17.02 Stroke completed -- waiting for pressure to equalize ... @00:54:27.79 Intake.select :close @00:54:27.94 SSV.dial :exhaust @00:54:28.81 Exhaust.select :open @00:54:28.93 SS.reconfigure SSexhale @00:54:28.98 SS.seek 0ml @00:54:38.59 Exhaust.select :close @00:54:38.71 SSV.dial CBV @00:54:38.74 CBV.dial SSV @00:54:39.62 Allowing 130s for 25.0ml of remaining 800.0ml @00:54:39.64 Intake.select :open @00:54:41.82 Intake @15.5psia, vacuum from 9.0 to 13.0psi @00:54:41.84 SS.reconfigure SSinhale.with maxSpeed: 35 @00:54:41.89 SS.seek 25ml @00:55:32.29 Stroke completed -- waiting for pressure to equalize ... @00:55:43.64 Intake.select :close @00:55:43.79 SSV.dial :exhaust @00:55:44.66 Exhaust.select :open @00:55:44.78 SS.reconfigure SSexhale @00:55:44.83 SS.seek 0ml @00:55:54.35 Exhaust.select :close @00:55:54.47 SSV.dial CBV @00:55:54.50 CBV.dial SSV @00:55:55.38 Allowing 130s for 25.0ml of remaining 775.0ml @00:55:55.40 Intake.select :open @00:55:57.58 Intake @15.5psia, vacuum from 9.0 to 13.0psi @00:55:57.60 SS.reconfigure SSinhale.with maxSpeed: 35 @00:55:57.65 SS.seek 25ml @00:56:14.67 delay 30 seconds @00:56:44.70 ATV.dial :waste,via: :air3 @00:56:46.57 AS.reconfigure ASconfig @00:56:46.61 AS.seek :empty @00:56:48.21 Stroke completed -- waiting for pressure to equalize ... @00:57:00.23 Intake.select :close @00:57:00.39 SSV.dial :exhaust @00:57:01.26 Exhaust.select :open @00:57:01.38 SS.reconfigure SSexhale @00:57:01.43 SS.seek 0ml @00:57:02.98 Parking Bleach in LysateSource line and SPEcolumn @00:57:02.99 ATV.dial :air3 @00:57:03.86 AS.reconfigure ASslow1 @00:57:03.91 AS.seek 0.029ml @00:57:10.24 ATV.dial :ElutionWater @00:57:10.86 AS.seek 0.059ml @00:57:11.13 Exhaust.select :close @00:57:11.25 SSV.dial CBV @00:57:11.28 CBV.dial SSV @00:57:12.15 Allowing 130s for 25.0ml of remaining 750.0ml @00:57:12.17 Intake.select :open @00:57:14.35 Intake @15.5psia, vacuum from 9.0 to 13.0psi @00:57:14.37 SS.reconfigure SSinhale.with maxSpeed: 35 @00:57:14.42 SS.seek 25ml @00:57:17.15 ATV.dial :air3 @00:57:17.80 AS.seek 0.069ml @00:57:20.09 delay 2 seconds @00:57:22.12 ATV.dial :bleach @00:57:22.63 AS.seek 0.769ml @00:58:04.77 Stroke completed -- waiting for pressure to equalize ... @00:58:17.39 Intake.select :close @00:58:17.54 SSV.dial :exhaust @00:58:18.41 Exhaust.select :open @00:58:18.53 SS.reconfigure SSexhale @00:58:18.58 SS.seek 0ml @00:58:28.18 Exhaust.select :close @00:58:28.30 SSV.dial CBV @00:58:28.33 CBV.dial SSV @00:58:29.23 Allowing 130s for 25.0ml of remaining 725.0ml @00:58:29.25 Intake.select :open @00:58:31.43 Intake @15.6psia, vacuum from 9.1 to 13.1psi @00:58:31.45 SS.reconfigure SSinhale.with maxSpeed: 35 @00:58:31.50 SS.seek 25ml @00:59:18.12 Vacuum too high -- waiting up to 40s @00:59:27.11 Intake @15.4psia, vacuum from 8.9 to 12.9psi @00:59:27.13 SS.reconfigure SSinhale.with maxSpeed: 32 @00:59:27.18 SS.seek 25ml @00:59:31.88 Stroke completed -- waiting for pressure to equalize ... @00:59:37.66 Intake.select :close @00:59:37.81 SSV.dial :exhaust @00:59:38.68 Exhaust.select :open @00:59:38.80 SS.reconfigure SSexhale @00:59:38.85 SS.seek 0ml @00:59:43.67 delay 3 seconds @00:59:46.70 ATV.dial :air3 @00:59:47.28 delay 3 seconds @00:59:48.59 Exhaust.select :close @00:59:48.71 SSV.dial CBV @00:59:48.74 CBV.dial SSV @00:59:49.62 Allowing 130s for 25.0ml of remaining 700.0ml @00:59:49.64 Intake.select :open @00:59:50.36 AS.seek 0.794ml @00:59:51.82 Intake @15.6psia, vacuum from 9.1 to 13.1psi @00:59:51.84 SS.reconfigure SSinhale.with maxSpeed: 32 @00:59:51.89 SS.seek 25ml @00:59:55.65 delay 3 seconds @00:59:58.68 ATV.dial :bleach @00:59:59.32 AS.seek 0.779ml @01:00:02.65 delay 3 seconds @01:00:05.68 ATV.dial :AIV @01:00:07.04 AS.reconfigure ASconfig @01:00:07.09 AS.seek 0.015ml @01:00:47.10 Stroke completed -- waiting for pressure to equalize ... @01:00:51.32 ATV.dial :waste @01:00:52.90 AS.seek :empty @01:00:54.10 ATV.dial :air3 @01:00:54.81 AS.reconfigure ASslow1 @01:00:54.86 AS.seek 0.009ml @01:00:57.21 delay 2 seconds @01:00:59.23 ATV.dial :bleach @01:00:59.81 Intake.select :close @01:00:59.86 AS.seek 0.579ml @01:01:00.03 SSV.dial :exhaust @01:01:01.46 Exhaust.select :open @01:01:01.58 SS.reconfigure SSexhale @01:01:01.63 SS.seek 0ml @01:01:11.26 Exhaust.select :close @01:01:11.38 SSV.dial CBV @01:01:11.41 CBV.dial SSV @01:01:12.30 Allowing 130s for 25.0ml of remaining 675.0ml @01:01:12.32 Intake.select :open @01:01:14.50 Intake @15.5psia, vacuum from 9.0 to 13.0psi @01:01:14.52 SS.reconfigure SSinhale.with maxSpeed: 32 @01:01:14.57 SS.seek 25ml @01:02:09.58 Stroke completed -- waiting for pressure to equalize ... @01:02:22.91 Intake.select :close @01:02:23.06 SSV.dial :exhaust @01:02:23.93 Exhaust.select :open @01:02:24.05 SS.reconfigure SSexhale @01:02:24.10 SS.seek 0ml @01:02:33.79 Exhaust.select :close @01:02:33.91 SSV.dial CBV @01:02:33.94 CBV.dial SSV @01:02:34.82 Allowing 130s for 25.0ml of remaining 650.0ml @01:02:34.84 Intake.select :open @01:02:37.02 Intake @15.6psia, vacuum from 9.1 to 13.1psi @01:02:37.04 SS.reconfigure SSinhale.with maxSpeed: 32 @01:02:37.08 SS.seek 25ml @01:02:54.81 delay 3 seconds @01:02:57.84 ATV.dial :air3 @01:02:58.42 delay 3 seconds @01:03:01.51 AS.seek 0.604ml @01:03:06.82 delay 3 seconds @01:03:09.85 ATV.dial :bleach @01:03:10.50 AS.seek 0.589ml @01:03:13.93 delay 3 seconds @01:03:16.95 ATV.dial :ASV @01:03:18.04 ASV.dial :wasteR @01:03:18.66 AS.seek 0ml @01:03:32.32 Stroke completed -- waiting for pressure to equalize ... @01:03:46.14 Intake.select :close @01:03:46.29 SSV.dial :exhaust @01:03:47.16 Exhaust.select :open @01:03:47.28 SS.reconfigure SSexhale @01:03:47.33 SS.seek 0ml @01:03:57.07 Exhaust.select :close @01:03:57.19 SSV.dial CBV @01:03:57.22 CBV.dial SSV @01:03:58.12 Allowing 130s for 25.0ml of remaining 625.0ml @01:03:58.14 Intake.select :open @01:04:00.32 Intake @15.6psia, vacuum from 9.1 to 13.1psi @01:04:00.34 SS.reconfigure SSinhale.with maxSpeed: 32 @01:04:00.39 SS.seek 25ml @01:04:55.27 Stroke completed -- waiting for pressure to equalize ... @01:05:09.20 Intake.select :close @01:05:09.35 SSV.dial :exhaust @01:05:10.22 Exhaust.select :open @01:05:10.34 SS.reconfigure SSexhale @01:05:10.40 SS.seek 0ml @01:05:17.42 delay 1:00 @01:05:19.99 Exhaust.select :close @01:05:20.11 SSV.dial CBV @01:05:20.14 CBV.dial SSV @01:05:21.01 Allowing 130s for 25.0ml of remaining 600.0ml @01:05:21.03 Intake.select :open @01:05:23.21 Intake @15.6psia, vacuum from 9.1 to 13.1psi @01:05:23.23 SS.reconfigure SSinhale.with maxSpeed: 32 @01:05:23.28 SS.seek 25ml @01:06:17.44 AFV.select :carrierSolution @01:06:17.54 delay 2 seconds @01:06:18.57 Stroke completed -- waiting for pressure to equalize ... @01:06:19.57 AS.reconfigure ASconfig @01:06:19.61 AS.seek 1ml @01:06:33.16 Intake.select :close @01:06:33.32 SSV.dial :exhaust @01:06:34.19 Exhaust.select :open @01:06:34.31 SS.reconfigure SSexhale @01:06:34.36 SS.seek 0ml @01:06:43.97 Exhaust.select :close @01:06:44.09 SSV.dial CBV @01:06:44.12 CBV.dial SSV @01:06:45.00 Allowing 130s for 25.0ml of remaining 575.0ml @01:06:45.02 Intake.select :open @01:06:47.20 Intake @15.6psia, vacuum from 9.1 to 13.1psi @01:06:47.22 SS.reconfigure SSinhale.with maxSpeed: 32 @01:06:47.27 SS.seek 25ml @01:07:17.40 delay 1 second @01:07:18.42 AFV.select :holdingCoil @01:07:18.52 delay 1 second @01:07:19.55 ATV.dial ASV @01:07:19.56 ASV.dial :wasteR @01:07:19.58 AS.reconfigure ASslow1 @01:07:19.63 AS.seek 0ml @01:07:42.55 Stroke completed -- waiting for pressure to equalize ... @01:07:57.13 Intake.select :close @01:07:57.28 SSV.dial :exhaust @01:07:58.15 Exhaust.select :open @01:07:58.27 SS.reconfigure SSexhale @01:07:58.32 SS.seek 0ml @01:08:08.07 Exhaust.select :close @01:08:08.19 SSV.dial CBV @01:08:08.22 CBV.dial SSV @01:08:09.65 Allowing 130s for 25.0ml of remaining 550.0ml @01:08:09.67 Intake.select :open @01:08:11.85 Intake @15.7psia, vacuum from 9.2 to 13.2psi @01:08:11.87 SS.reconfigure SSinhale.with maxSpeed: 32 @01:08:11.92 SS.seek 25ml @01:09:06.83 Stroke completed -- waiting for pressure to equalize ... @01:09:21.43 Intake.select :close @01:09:21.58 SSV.dial :exhaust @01:09:22.47 Exhaust.select :open @01:09:22.59 SS.reconfigure SSexhale @01:09:22.64 SS.seek 0ml @01:09:31.88 Exhaust.select :close @01:09:32.00 SSV.dial CBV @01:09:32.03 CBV.dial SSV @01:09:32.91 Allowing 130s for 25.0ml of remaining 525.0ml @01:09:32.93 Intake.select :open @01:09:35.11 Intake @15.7psia, vacuum from 9.2 to 13.2psi @01:09:35.13 SS.reconfigure SSinhale.with maxSpeed: 32 @01:09:35.18 SS.seek 25ml @01:10:30.91 Stroke completed -- waiting for pressure to equalize ... @01:10:40.97 delay 20 seconds @01:10:46.63 Intake.select :close @01:10:46.78 SSV.dial :exhaust @01:10:47.66 Exhaust.select :open @01:10:47.78 SS.reconfigure SSexhale @01:10:47.83 SS.seek 0ml @01:10:57.39 Exhaust.select :close @01:10:57.51 SSV.dial CBV @01:10:57.54 CBV.dial SSV @01:10:58.41 Allowing 130s for 25.0ml of remaining 500.0ml @01:10:58.43 Intake.select :open @01:11:00.61 Intake @15.7psia, vacuum from 9.2 to 13.2psi @01:11:00.63 SS.reconfigure SSinhale.with maxSpeed: 32 @01:11:00.68 SS.seek 25ml @01:11:01.00 ATV.dial :air3 @01:11:02.66 AS.seek 0.59ml @01:11:55.91 Stroke completed -- waiting for pressure to equalize ... @01:12:11.73 Intake.select :close @01:12:11.89 SSV.dial :exhaust @01:12:12.75 Exhaust.select :open @01:12:12.87 SS.reconfigure SSexhale @01:12:12.92 SS.seek 0ml @01:12:22.57 Exhaust.select :close @01:12:22.69 SSV.dial CBV @01:12:22.72 CBV.dial SSV @01:12:23.61 Allowing 130s for 25.0ml of remaining 475.0ml @01:12:23.63 Intake.select :open @01:12:25.80 Intake @15.7psia, vacuum from 9.2 to 13.2psi @01:12:25.82 SS.reconfigure SSinhale.with maxSpeed: 32 @01:12:25.87 SS.seek 25ml @01:13:01.59 delay 3 seconds @01:13:04.62 ATV.dial :ASV @01:13:06.12 ASV.dial :wasteR @01:13:06.21 AS.seek 0.09ml @01:13:20.96 Stroke completed -- waiting for pressure to equalize ... @01:13:36.71 Intake.select :close @01:13:36.87 SSV.dial :exhaust @01:13:37.74 Exhaust.select :open @01:13:37.86 SS.reconfigure SSexhale @01:13:37.91 SS.seek 0ml @01:13:47.19 Exhaust.select :close @01:13:47.31 SSV.dial CBV @01:13:47.34 CBV.dial SSV @01:13:48.22 Allowing 130s for 25.0ml of remaining 450.0ml @01:13:48.24 Intake.select :open @01:13:50.42 Intake @15.7psia, vacuum from 9.2 to 13.2psi @01:13:50.44 SS.reconfigure SSinhale.with maxSpeed: 32 @01:13:50.49 SS.seek 25ml @01:14:46.30 Stroke completed -- waiting for pressure to equalize ... @01:14:47.15 delay 30 seconds @01:15:02.71 Intake.select :close @01:15:02.87 SSV.dial :exhaust @01:15:03.74 Exhaust.select :open @01:15:03.86 SS.reconfigure SSexhale @01:15:03.91 SS.seek 0ml @01:15:13.58 Exhaust.select :close @01:15:13.70 SSV.dial CBV @01:15:14.20 CBV.dial SSV @01:15:15.19 Allowing 130s for 25.0ml of remaining 425.0ml @01:15:15.21 Intake.select :open @01:15:17.17 ATV.dial :extSrc @01:15:17.39 Intake @15.7psia, vacuum from 9.2 to 13.2psi @01:15:17.41 SS.reconfigure SSinhale.with maxSpeed: 32 @01:15:17.46 SS.seek 25ml @01:15:17.99 delay 3 seconds @01:15:21.02 ATV.dial :ElutionWater @01:15:22.21 AS.seek 0.12ml @01:15:28.54 ATV.dial :air3 @01:15:29.20 AS.seek 0.13ml @01:15:31.48 delay 2 seconds @01:15:33.50 ATV.dial :bleach @01:15:33.99 AS.seek 0.23ml @01:15:54.38 delay 3 seconds @01:15:57.41 ATV.dial :air3 @01:15:57.98 delay 3 seconds @01:16:01.07 AS.seek 0.255ml @01:16:06.38 delay 3 seconds @01:16:09.41 ATV.dial :bleach @01:16:10.06 AS.seek 0.24ml @01:16:12.75 Stroke completed -- waiting for pressure to equalize ... @01:16:13.42 delay 3 seconds @01:16:16.51 ATV.dial :ASV @01:16:17.60 ASV.dial :SPEdelivery @01:16:17.99 AFV.select :carrierSolution @01:16:18.10 delay 2 seconds @01:16:20.13 AS.reconfigure ASconfig @01:16:20.17 AS.seek 0.5ml @01:16:29.21 Intake.select :close @01:16:29.36 SSV.dial :exhaust @01:16:30.24 Exhaust.select :open @01:16:30.36 SS.reconfigure SSexhale @01:16:30.41 SS.seek 0ml @01:16:35.43 delay 1 second @01:16:36.45 AFV.select :holdingCoil @01:16:36.61 AS.reconfigure ASslow1 @01:16:36.66 AS.seek 0.17ml @01:16:40.22 Exhaust.select :close @01:16:40.34 SSV.dial CBV @01:16:40.37 CBV.dial SSV @01:16:41.25 Allowing 130s for 25.0ml of remaining 400.0ml @01:16:41.27 Intake.select :open @01:16:43.45 Intake @15.7psia, vacuum from 9.2 to 13.2psi @01:16:43.47 SS.reconfigure SSinhale.with maxSpeed: 32 @01:16:43.52 SS.seek 25ml @01:17:38.85 Stroke completed -- waiting for pressure to equalize ... @01:17:43.36 SPE.hold 2:46:40,95C @01:17:43.41 delay 3:00 @01:17:55.32 Intake.select :close @01:17:55.48 SSV.dial :exhaust @01:17:56.38 Exhaust.select :open @01:17:56.50 SS.reconfigure SSexhale @01:17:56.55 SS.seek 0ml @01:18:05.88 Exhaust.select :close @01:18:06.00 SSV.dial CBV @01:18:06.03 CBV.dial SSV @01:18:06.95 Allowing 130s for 25.0ml of remaining 375.0ml @01:18:06.97 Intake.select :open @01:18:09.14 Intake @15.7psia, vacuum from 9.2 to 13.2psi @01:18:09.16 SS.reconfigure SSinhale.with maxSpeed: 32 @01:18:09.21 SS.seek 25ml @01:19:05.02 Stroke completed -- waiting for pressure to equalize ... @01:19:22.03 Intake.select :close @01:19:22.18 SSV.dial :exhaust @01:19:23.08 Exhaust.select :open @01:19:23.20 SS.reconfigure SSexhale @01:19:23.25 SS.seek 0ml @01:19:33.13 Exhaust.select :close @01:19:33.25 SSV.dial CBV @01:19:33.28 CBV.dial SSV @01:19:34.19 Allowing 130s for 25.0ml of remaining 350.0ml @01:19:34.21 Intake.select :open @01:19:36.39 Intake @15.8psia, vacuum from 9.3 to 13.3psi @01:19:36.41 SS.reconfigure SSinhale.with maxSpeed: 32 @01:19:36.46 SS.seek 25ml @01:20:31.47 Stroke completed -- waiting for pressure to equalize ... @01:20:43.51 AFV.select :carrierSolution @01:20:43.62 delay 2 seconds @01:20:45.65 AS.reconfigure ASconfig @01:20:45.69 AS.seek 1ml @01:20:47.36 Intake.select :close @01:20:47.51 SSV.dial :exhaust @01:20:48.41 Exhaust.select :open @01:20:48.53 SS.reconfigure SSexhale @01:20:48.58 SS.seek 0ml @01:20:58.34 Exhaust.select :close @01:20:58.46 SSV.dial CBV @01:20:58.49 CBV.dial SSV @01:20:59.41 Allowing 130s for 25.0ml of remaining 325.0ml @01:20:59.43 Intake.select :open @01:21:01.61 Intake @15.8psia, vacuum from 9.3 to 13.3psi @01:21:01.63 SS.reconfigure SSinhale.with maxSpeed: 32 @01:21:01.68 SS.seek 25ml @01:21:33.70 delay 1 second @01:21:34.72 AFV.select :holdingCoil @01:21:34.82 delay 1 second @01:21:35.85 ASV.dial :wasteD @01:21:36.97 AS.seek 0ml @01:21:56.70 Stroke completed -- waiting for pressure to equalize ... @01:22:13.21 Intake.select :close @01:22:13.36 SSV.dial :exhaust @01:22:14.27 Exhaust.select :open @01:22:14.39 SS.reconfigure SSexhale @01:22:14.44 SS.seek 0ml @01:22:24.16 Exhaust.select :close @01:22:24.28 SSV.dial CBV @01:22:24.31 CBV.dial SSV @01:22:25.22 Allowing 130s for 25.0ml of remaining 300.0ml @01:22:25.24 Intake.select :open @01:22:27.42 Intake @15.7psia, vacuum from 9.2 to 13.2psi @01:22:27.44 SS.reconfigure SSinhale.with maxSpeed: 32 @01:22:27.49 SS.seek 25ml @01:22:34.74 Priming Eluate @01:22:34.75 AFV.select :carrierSolution @01:22:34.86 delay 2 seconds @01:22:36.90 AS.seek 0.5ml @01:23:05.93 delay 1 second @01:23:06.95 AFV.select :holdingCoil @01:23:07.05 ATV.dial ASV @01:23:07.07 ASV.dial :SPErecovery @01:23:07.70 Swish #1 of 2 @01:23:07.78 AS.reconfigure ASslow0 @01:23:07.84 AS.seek 0.6ml @01:23:18.22 AS.seek 0.5ml @01:23:22.77 Stroke completed -- waiting for pressure to equalize ... @01:23:28.56 Swish #2 of 2 @01:23:28.64 AS.seek 0.6ml @01:23:39.23 AS.seek 0.5ml @01:23:39.85 Intake.select :close @01:23:40.00 SSV.dial :exhaust @01:23:40.89 Exhaust.select :open @01:23:41.01 SS.reconfigure SSexhale @01:23:41.06 SS.seek 0ml @01:23:49.62 delay 3 seconds @01:23:50.36 Exhaust.select :close @01:23:50.48 SSV.dial CBV @01:23:50.51 CBV.dial SSV @01:23:51.41 Allowing 130s for 25.0ml of remaining 275.0ml @01:23:51.43 Intake.select :open @01:23:52.70 AS.reconfigure ASslow1 @01:23:52.76 AS.seek 0.684ml @01:23:53.61 Intake @15.8psia, vacuum from 9.3 to 13.3psi @01:23:53.63 SS.reconfigure SSinhale.with maxSpeed: 32 @01:23:53.68 SS.seek 25ml @01:24:30.04 delay 3 seconds @01:24:33.07 Eluate primed @01:24:33.08 ATV.dial :waste2 @01:24:33.75 AS.reconfigure ASnorm @01:24:33.80 AS.seek 0ml @01:24:49.59 Stroke completed -- waiting for pressure to equalize ... @01:25:07.96 Intake.select :close @01:25:08.12 SSV.dial :exhaust @01:25:08.60 SPE.coast @01:25:08.68 ASV.dial :SPErecovery @01:25:08.70 Setting up PCR for IPc -- writing /var/log/esp/IPc19may2401h1000ml.pcr @01:25:08.72 Building PCR Slug from ASV with MM1 mix, PP1 primer @01:25:08.73 Priming with carrier @01:25:08.74 ATV.dial ARV @01:25:09.00 Exhaust.select :open @01:25:09.12 SS.reconfigure SSexhale @01:25:09.17 SS.seek 0ml @01:25:09.75 ARV.dial :carrier @01:25:10.15 AS.seek 0.5ml @01:25:18.80 Exhaust.select :close @01:25:18.92 SSV.dial CBV @01:25:18.95 CBV.dial SSV @01:25:19.83 Allowing 130s for 25.0ml of remaining 250.0ml @01:25:19.85 Intake.select :open @01:25:22.03 Intake @15.9psia, vacuum from 9.4 to 13.4psi @01:25:22.05 SS.reconfigure SSinhale.with maxSpeed: 32 @01:25:22.10 SS.seek 25ml @01:25:36.24 AFV.select :carrierSolution @01:25:36.35 delay 2 seconds @01:25:38.39 AS.reconfigure ASconfig @01:25:38.44 AS.seek 1ml @01:26:07.46 delay 1 second @01:26:08.48 AFV.select :holdingCoil @01:26:08.58 delay 1 second @01:26:09.61 ATV.dial :waste @01:26:11.21 AS.reconfigure ASnorm @01:26:11.26 AS.seek :empty @01:26:17.28 Stroke completed -- waiting for pressure to equalize ... @01:26:34.39 Intake.select :close @01:26:34.55 SSV.dial :exhaust @01:26:35.42 Exhaust.select :open @01:26:35.54 SS.reconfigure SSexhale @01:26:35.59 SS.seek 0ml @01:26:44.86 Exhaust.select :close @01:26:44.98 SSV.dial CBV @01:26:45.01 CBV.dial SSV @01:26:45.87 Allowing 130s for 25.0ml of remaining 225.0ml @01:26:45.89 Intake.select :open @01:26:48.07 Intake @15.8psia, vacuum from 9.3 to 13.3psi @01:26:48.09 SS.reconfigure SSinhale.with maxSpeed: 32 @01:26:48.13 SS.seek 25ml @01:27:01.98 AFV.select :carrierSolution @01:27:02.09 delay 2 seconds @01:27:04.13 AS.reconfigure ASconfig @01:27:04.17 AS.seek 0.5ml @01:27:33.26 delay 1 second @01:27:34.28 AFV.select :holdingCoil @01:27:34.39 Building Slug @01:27:34.40 ATV.dial :ARV @01:27:36.10 delay 2 seconds @01:27:38.13 ARV.dial :air1 @01:27:38.55 delay 2 seconds @01:27:40.64 AS.reconfigure ASslow2 @01:27:40.70 AS.seek 0.506ml @01:27:43.45 Stroke completed -- waiting for pressure to equalize ... @01:27:47.28 delay 2 seconds @01:27:49.30 ARV.dial :MM1 @01:27:49.92 delay 2 seconds @01:27:51.95 Adding MM1 @01:27:52.02 AS.seek 0.524ml @01:28:01.18 Intake.select :close @01:28:01.34 SSV.dial :exhaust @01:28:02.22 Exhaust.select :open @01:28:02.34 SS.reconfigure SSexhale @01:28:02.39 SS.seek 0ml @01:28:10.43 delay 25 seconds @01:28:12.19 Exhaust.select :close @01:28:12.31 SSV.dial CBV @01:28:12.34 CBV.dial SSV @01:28:13.22 Allowing 130s for 25.0ml of remaining 200.0ml @01:28:13.24 Intake.select :open @01:28:15.42 Intake @15.9psia, vacuum from 9.4 to 13.4psi @01:28:15.44 SS.reconfigure SSinhale.with maxSpeed: 32 @01:28:15.49 SS.seek 25ml @01:28:35.46 ARV.dial :PP1 @01:28:35.86 Adding PP1 @01:28:35.87 delay 2 seconds @01:28:37.96 AS.seek 0.53ml @01:28:44.13 delay 10 seconds @01:28:54.16 ARV.dial :air1 @01:28:54.57 adding ARV air @01:28:54.58 delay 2 seconds @01:28:56.67 AS.seek 0.536ml @01:29:03.07 delay 5 seconds @01:29:08.10 ARV.dial :carrier @01:29:08.52 delay 7 seconds @01:29:10.73 Stroke completed -- waiting for pressure to equalize ... @01:29:15.61 AS.seek 0.594ml @01:29:28.48 Intake.select :close @01:29:28.64 SSV.dial :exhaust @01:29:29.51 Exhaust.select :open @01:29:29.64 SS.reconfigure SSexhale @01:29:29.69 SS.seek 0ml @01:29:39.31 Exhaust.select :close @01:29:39.43 SSV.dial CBV @01:29:39.46 CBV.dial SSV @01:29:40.31 Allowing 130s for 25.0ml of remaining 175.0ml @01:29:40.33 Intake.select :open @01:29:42.51 Intake @15.8psia, vacuum from 9.3 to 13.3psi @01:29:42.53 SS.reconfigure SSinhale.with maxSpeed: 32 @01:29:42.58 SS.seek 25ml @01:30:14.09 delay 5 seconds @01:30:19.12 ATV.dial :ASV @01:30:19.54 delay 2 seconds @01:30:21.64 AS.seek 0.6ml @01:30:27.88 delay 4 seconds @01:30:31.91 ATV.dial ARV @01:30:32.57 delay 2 seconds @01:30:34.60 ARV.dial :carrier @01:30:34.62 delay 2 seconds @01:30:36.71 AS.seek 0.65ml @01:30:37.72 Stroke completed -- waiting for pressure to equalize ... @01:30:55.43 Intake.select :close @01:30:55.59 SSV.dial :exhaust @01:30:56.47 Exhaust.select :open @01:30:56.59 SS.reconfigure SSexhale @01:30:56.64 SS.seek 0ml @01:31:05.94 Exhaust.select :close @01:31:06.06 SSV.dial CBV @01:31:06.09 CBV.dial SSV @01:31:06.97 Allowing 130s for 25.0ml of remaining 150.0ml @01:31:06.99 Intake.select :open @01:31:09.17 Intake @15.8psia, vacuum from 9.3 to 13.3psi @01:31:09.19 SS.reconfigure SSinhale.with maxSpeed: 32 @01:31:09.24 SS.seek 25ml @01:31:27.35 delay 4 seconds @01:31:31.38 ATV.dial PCR @01:31:32.12 ASV.dial :waste1 @01:31:32.93 ARV.dial :air1 @01:31:33.46 Positioning Slug by 0.324ml @01:31:33.55 AS.seek 0.326ml @01:32:05.03 Stroke completed -- waiting for pressure to equalize ... @01:32:23.39 Intake.select :close @01:32:23.55 SSV.dial :exhaust @01:32:24.42 Exhaust.select :open @01:32:24.54 SS.reconfigure SSexhale @01:32:24.59 SS.seek 0ml @01:32:34.28 Exhaust.select :close @01:32:34.40 SSV.dial CBV @01:32:34.43 CBV.dial SSV @01:32:35.31 Allowing 130s for 25.0ml of remaining 125.0ml @01:32:35.33 Intake.select :open @01:32:37.51 Intake @15.9psia, vacuum from 9.4 to 13.4psi @01:32:37.53 SS.reconfigure SSinhale.with maxSpeed: 32 @01:32:37.58 SS.seek 25ml @01:33:32.58 Stroke completed -- waiting for pressure to equalize ... @01:33:49.73 Intake.select :close @01:33:49.88 SSV.dial :exhaust @01:33:50.73 Exhaust.select :open @01:33:50.85 SS.reconfigure SSexhale @01:33:50.90 SS.seek 0ml @01:34:00.45 Exhaust.select :close @01:34:00.57 SSV.dial CBV @01:34:00.60 CBV.dial SSV @01:34:01.47 Allowing 130s for 25.0ml of remaining 100.0ml @01:34:01.49 Intake.select :open @01:34:03.67 Intake @15.9psia, vacuum from 9.4 to 13.4psi @01:34:03.68 SS.reconfigure SSinhale.with maxSpeed: 32 @01:34:03.73 SS.seek 25ml @01:34:58.92 Stroke completed -- waiting for pressure to equalize ... @01:35:17.22 Intake.select :close @01:35:17.38 SSV.dial :exhaust @01:35:18.25 Exhaust.select :open @01:35:18.37 SS.reconfigure SSexhale @01:35:18.42 SS.seek 0ml @01:35:28.23 Exhaust.select :close @01:35:28.35 SSV.dial CBV @01:35:28.38 CBV.dial SSV @01:35:29.25 Allowing 130s for 25.0ml of remaining 75.0ml @01:35:29.27 Intake.select :open @01:35:31.45 Intake @15.9psia, vacuum from 9.4 to 13.4psi @01:35:31.47 SS.reconfigure SSinhale.with maxSpeed: 32 @01:35:31.52 SS.seek 25ml @01:36:26.78 Stroke completed -- waiting for pressure to equalize ... @01:36:45.15 Intake.select :close @01:36:45.30 SSV.dial :exhaust @01:36:46.15 Exhaust.select :open @01:36:46.27 SS.reconfigure SSexhale @01:36:46.32 SS.seek 0ml @01:36:56.03 Exhaust.select :close @01:36:56.15 SSV.dial CBV @01:36:56.18 CBV.dial SSV @01:36:57.04 Allowing 130s for 25.0ml of remaining 50.0ml @01:36:57.06 Intake.select :open @01:36:59.23 Intake @15.9psia, vacuum from 9.4 to 13.4psi @01:36:59.25 SS.reconfigure SSinhale.with maxSpeed: 32 @01:36:59.30 SS.seek 25ml @01:36:59.92 delay 5 seconds @01:37:04.95 ATV.dial ARV @01:37:05.74 ASV.dial :PCRlock @01:37:06.12 delay 5 seconds @01:37:11.16 Running IPc ProfileIPc thermocycling @01:37:11.17 PCR.program ProfileIPc @01:37:11.31 ProfileIPc should complete in < 1:50:49 @01:37:11.33 PCR.run 2:23:31.25 @01:37:54.29 Stroke completed -- waiting for pressure to equalize ... @01:38:12.75 Intake.select :close @01:38:12.91 SSV.dial :exhaust @01:38:13.78 Exhaust.select :open @01:38:13.90 SS.reconfigure SSexhale @01:38:13.95 SS.seek 0ml @01:38:23.20 Exhaust.select :close @01:38:23.32 SSV.dial CBV @01:38:23.35 CBV.dial SSV @01:38:24.23 Allowing 130s for 25.0ml of remaining 25.0ml @01:38:24.25 Intake.select :open @01:38:26.43 Intake @15.9psia, vacuum from 9.4 to 13.4psi @01:38:26.45 SS.reconfigure SSinhale.with maxSpeed: 32 @01:38:26.50 SS.seek 24.97ml @01:39:22.26 Stroke completed -- waiting for pressure to equalize ... @01:39:41.28 Intake.select :close @01:39:41.43 SSV.dial :exhaust @01:39:42.32 Exhaust.select :open @01:39:42.44 SS.reconfigure SSexhale @01:39:42.49 SS.seek 0ml @01:39:52.18 Exhaust.select :close @01:39:52.31 Sampled 1000.0ml @01:39:52.33 Drying puck before fixing for 4:00 @01:39:52.34 CBV.dial :waste2b @01:39:53.23 delay 1:00 @01:39:55.31 Gate.power :sampler,:OFF @01:40:53.25 CSV.dial :air @01:40:53.79 CS.reconfigure CSfast @01:40:53.84 CS.seek 10ml @01:41:20.67 Pumping wcr puck from tube 2 up to 55psi... @01:41:20.69 CBV.dial SSV @01:41:22.15 CBV.dial between CSV and waste2b @01:41:23.27 CSV.dial between air and CTV @01:41:23.66 CS.seek 7.172ml @01:41:32.03 CTV.dial CSV,avoiding: :air @01:41:32.06 CSV.dial CTV @01:41:33.00 CS.reconfigure CSpressurize @01:41:33.05 CS.seek 0ml @01:41:49.22 CBV.dial :waste2b @01:41:49.60 CS.reconfigure CSconfig @01:41:49.65 CS.seek :empty @01:41:53.86 delay 1:00 @01:42:53.89 CTV.dial :air,avoiding: :intake @01:42:55.46 Fix #1 of 2 (with rnal) for 2:00 @01:42:55.47 CSV.dial CSR @01:42:56.06 Pulling 2ml of CSR.rnal @01:42:56.07 CSR.select :rnal @01:42:56.20 CS.seek 2ml @01:43:02.33 delay 5 seconds @01:43:07.36 CSR.select :air @01:43:07.49 CS.reconfigure CSfast @01:43:07.54 CS.seek 10ml @01:43:29.49 Pumping wcr puck from tube 2 up to 55psi... @01:43:29.51 CBV.dial SSV @01:43:31.51 CBV.dial between CSV and waste2b @01:43:32.63 CSV.dial between air and CTV @01:43:33.37 CS.seek 7.072ml @01:43:41.96 CTV.dial CSV,avoiding: :air @01:43:41.99 CSV.dial CTV @01:43:43.65 CS.reconfigure CSpressurize @01:43:43.70 CS.seek 0ml @01:44:01.52 delay 2:00 @01:46:01.54 CBV.dial :waste2b @01:46:01.94 CS.reconfigure CSconfig @01:46:01.98 CS.seek :empty @01:46:04.52 delay 1:00 @01:47:04.55 CTV.dial :air,avoiding: :intake @01:47:06.12 Fix #2 of 2 (with rnal) for 2:00 @01:47:06.13 CSV.dial CSR @01:47:06.71 Pulling 2ml of CSR.rnal @01:47:06.72 CSR.select :rnal @01:47:06.84 CS.seek 2ml @01:47:12.93 delay 5 seconds @01:47:17.96 CSR.select :air @01:47:18.09 CS.reconfigure CSfast @01:47:18.14 CS.seek 10ml @01:47:39.83 Pumping wcr puck from tube 2 up to 55psi... @01:47:39.85 CBV.dial SSV @01:47:41.29 CBV.dial between CSV and waste2b @01:47:42.42 CSV.dial between air and CTV @01:47:43.15 CS.seek 6.999ml @01:47:52.03 CTV.dial CSV,avoiding: :air @01:47:52.06 CSV.dial CTV @01:47:53.67 CS.reconfigure CSpressurize @01:47:53.72 CS.seek 0ml @01:48:10.79 delay 2:00 @01:50:10.82 CBV.dial :waste2b @01:50:11.20 CS.reconfigure CSconfig @01:50:11.25 CS.seek :empty @01:50:13.86 delay 1:00 @01:51:13.89 CTV.dial :air,avoiding: :intake @01:51:15.53 Storing wcr puck in Collection Clamp to tube 1 @01:51:15.73 SC.reconfigure SCtight @01:51:15.78 SC.seek 1 @01:51:17.93 SC.reconfigure SCloose @01:51:18.17 Elbow.reconfigure TightElbow @01:51:18.22 Elbow.seek 1 @01:51:20.16 Forearm.reconfigure TightForearm @01:51:20.21 Forearm.seek 1 + 700 counts @01:51:21.27 SE.reconfigure SEpick @01:51:21.34 SE.seek beamBreak @01:51:24.45 SE.configure SEconfig @01:51:24.63 Elbow.reconfigure LimpElbow @01:51:24.69 Forearm.reconfigure LooseForearm @01:51:24.74 Forearm.seek 1 @01:51:25.09 Elbow.hold @01:51:25.17 Elbow.reconfigure TightElbow @01:51:25.28 SE.seek 1.07 Pucks below Top Plate @01:51:25.89 Forearm.force -20 @01:51:25.94 Forearm.seek 1 + 319 counts @01:51:26.29 Forearm.reconfigure TightForearm @01:51:26.34 Forearm.seek 1 + 700 counts @01:51:26.82 SE.seek 2.07 Pucks below Top Plate @01:51:27.56 Forearm.reconfigure LooseForearm @01:51:27.62 Forearm.seek :retract @01:51:28.55 Elbow.seek CC @01:51:30.20 Elbow.reconfigure LooseElbow @01:51:30.31 Forearm.seek CC @01:51:31.37 Hand.close @01:51:32.15 CC.seek :open @01:51:36.72 Forearm.seek :retract @01:51:37.76 Elbow.reconfigure TightElbow @01:51:37.81 Elbow.seek 1 @01:51:39.75 Elbow.reconfigure LimpElbow @01:51:39.81 Forearm.seek 1 @01:51:40.83 Elbow.hold @01:51:40.91 Elbow.reconfigure TightElbow @01:51:40.96 Hand.open @01:51:41.90 Forearm.force -20 @01:51:41.94 Forearm.seek 1 + 350 counts @01:51:42.85 Forearm.reconfigure TightForearm @01:51:42.91 Forearm.seek 1 + 700 counts @01:51:43.33 SE.seek :down @01:51:44.50 Forearm.reconfigure LooseForearm @01:51:44.55 Forearm.seek :retract @01:51:46.00 SE.coast @01:51:46.04 SC.coast @01:51:46.08 Starting finalPurge @01:51:46.10 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @01:51:46.18 Hand.open @01:51:46.53 Elbow.seek FlushPuck::Garage @01:51:48.54 Elbow.reconfigure LooseElbow @01:51:48.65 Forearm.seek :garage @01:51:49.70 Hand.close @01:51:50.53 Forearm.seek :retract @01:51:51.60 Elbow.reconfigure TightElbow @01:51:51.64 Elbow.seek CC @01:51:54.57 Elbow.reconfigure LooseElbow @01:51:54.67 Forearm.reconfigure TightForearm @01:51:54.72 Forearm.seek CC @01:51:56.06 CC.seek :closed @01:52:00.33 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.47 @01:52:00.41 Hand.open @01:52:01.22 Forearm.reconfigure LooseForearm @01:52:01.26 Forearm.seek :retract @01:52:02.31 Flushing the Collection Valve::Manifold @01:52:02.46 CSV.dial CSR @01:52:03.03 Pulling 5ml of CSR.flush @01:52:03.04 CSR.select :flush @01:52:03.17 CS.seek 5ml @01:52:17.27 delay 5 seconds @01:52:22.29 CSR.select :air @01:52:22.46 CS.reconfigure CSfast @01:52:22.51 CS.seek 7.5ml @01:52:29.99 CSV.dial :waste @01:52:31.52 CS.reconfigure CSconfig @01:52:31.57 CS.seek 0ml @01:52:51.77 Flushing lower manifold's waste1 port @01:52:51.91 CSV.dial :flush @01:52:52.42 CS.reconfigure CSfast @01:52:52.47 CS.seek 5ml @01:53:06.37 delay 5 seconds @01:53:11.39 CSV.dial :air @01:53:11.98 CS.seek 7ml @01:53:18.71 CSV.dial CTV @01:53:18.75
CTV.dial CSV @01:53:20.38 CBV.dial :waste1 @01:53:21.27 CS.seek 0ml @01:53:40.27 Flushing lower manifold's waste2b port @01:53:40.44 CSV.dial :flush @01:53:41.31 CS.seek 5ml @01:53:55.15 delay 5 seconds @01:54:00.17 CSV.dial :air @01:54:00.77 CS.seek 7ml @01:54:06.87 CSV.dial CTV @01:54:06.90
CTV.dial CSV @01:54:07.41 CBV.dial :waste2b @01:54:08.30 CS.seek 0ml @01:54:27.34 Flushing lower manifold's waste1 port @01:54:27.36 CSV.dial CSR @01:54:27.94 Pulling 4ml of CSR.mfbkill @01:54:27.95 CSR.select :mfbkill @01:54:28.07 CS.reconfigure CSconfig @01:54:28.12 CS.seek 4ml @01:54:39.49 delay 5 seconds @01:54:44.51 CSR.select :air @01:54:44.64 CS.reconfigure CSfast @01:54:44.69 CS.seek 10ml @01:55:01.18 CSV.dial CTV @01:55:01.22
CTV.dial CSV @01:55:01.76 CBV.dial :waste1 @01:55:02.67 CS.seek 0ml @01:55:29.62 Flushing lower manifold's waste2b port @01:55:29.64 CSV.dial CSR @01:55:30.36 Pulling 4ml of CSR.mfbkill @01:55:30.37 CSR.select :mfbkill @01:55:30.49 CS.reconfigure CSconfig @01:55:30.54 CS.seek 4ml @01:55:41.81 delay 5 seconds @01:55:46.83 CSR.select :air @01:55:46.96 CS.reconfigure CSfast @01:55:47.01 CS.seek 10ml @01:56:03.27 CSV.dial CTV @01:56:03.31
CTV.dial CSV @01:56:03.84 CBV.dial :waste2b @01:56:04.74 CS.seek 0ml @01:56:31.46 Killing upper manifold @01:56:31.48 CSV.dial CSR @01:56:32.06 Pulling 4ml of CSR.mfbkill @01:56:32.07 CSR.select :mfbkill @01:56:32.19 CS.reconfigure CSconfig @01:56:32.24 CS.seek 4ml @01:56:43.53 delay 5 seconds @01:56:48.55 CSR.select :air @01:56:48.68 CS.reconfigure CSfast @01:56:48.73 CS.seek 10ml @01:57:05.21 CSV.dial CBV @01:57:05.25
CBV.dial CSV @01:57:06.49 CTV.dial :air @01:57:08.07 CS.seek 0ml @01:57:34.87 delay 7 seconds @01:57:41.90 CS.seek 3ml @01:57:50.56 CSV.dial :waste @01:57:51.65 CS.seek 0ml @01:58:00.28 Flushing upper manifold @01:58:00.43 CSV.dial :flush @01:58:00.94 CS.seek 5ml @01:58:14.90 delay 5 seconds @01:58:19.92 CSV.dial :air @01:58:20.51 CS.seek 7ml @01:58:26.53 CSV.dial CBV @01:58:26.57
CBV.dial CSV @01:58:27.79 CTV.dial :air @01:58:27.82 CS.seek 0ml @01:58:46.68 delay 7 seconds @01:58:53.71 CS.seek 3ml @01:59:02.46 CSV.dial :waste @01:59:02.99 CS.seek 0ml @01:59:11.81 CSV.dial :flush @01:59:12.32 CS.seek 3ml @01:59:21.03 delay 5 seconds @01:59:26.05 CSV.dial :air @01:59:26.67 CS.seek 5ml @01:59:32.84 CSV.dial :air @01:59:32.86 CS.seek 0ml @01:59:46.62 delay 7 seconds @01:59:53.65 CS.seek 3ml @02:00:02.37 CSV.dial :waste @02:00:03.26 CS.seek 0ml @02:00:11.87 Flushing lower manifold's waste1 port @02:00:12.01 CSV.dial :flush @02:00:12.54 CS.seek 5ml @02:00:26.43 delay 5 seconds @02:00:31.45 CSV.dial :air @02:00:32.04 CS.seek 7ml @02:00:38.25 CSV.dial CTV @02:00:38.28
CTV.dial CSV @02:00:39.93 CBV.dial :waste1 @02:00:40.46 CS.seek 0ml @02:00:59.28 Flushing lower manifold's waste2b port @02:00:59.42 CSV.dial :flush @02:01:00.32 CS.seek 5ml @02:01:14.15 delay 5 seconds @02:01:19.17 CSV.dial :air @02:01:19.78 CS.seek 7ml @02:01:25.97 CSV.dial CTV @02:01:26.01
CTV.dial CSV @02:01:26.53 CBV.dial :waste2b @02:01:27.43 CS.seek 0ml @02:01:46.50 Garaging FlushPuck in Collection Clamp @02:01:46.59 Elbow.reconfigure TightElbow @02:01:46.63 Elbow.seek CC @02:01:46.88 Elbow.reconfigure LooseElbow @02:01:46.99 Forearm.seek CC @02:01:48.06 Hand.close @02:01:49.29 CC.seek :open @02:01:53.76 Forearm.seek :retract @02:01:54.83 Elbow.reconfigure TightElbow @02:01:54.88 Elbow.seek :garage @02:01:58.13 Elbow.reconfigure LooseElbow @02:01:58.24 Forearm.reconfigure TightForearm @02:01:58.29 Forearm.seek :garage @02:01:59.46 Hand.open @02:02:00.23 Forearm.reconfigure LooseForearm @02:02:00.27 Forearm.seek :retract @02:02:01.31 Starting initialPurge @02:02:01.33 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @02:02:01.40 Hand.open @02:02:01.72 Elbow.reconfigure TightElbow @02:02:01.77 Elbow.seek FlushPuck::Garage @02:02:01.97 Elbow.reconfigure LooseElbow @02:02:02.08 Forearm.seek :garage @02:02:03.12 Hand.close @02:02:04.04 Forearm.seek :retract @02:02:05.10 Elbow.reconfigure TightElbow @02:02:05.14 Elbow.seek CC @02:02:08.11 Elbow.reconfigure LooseElbow @02:02:08.21 Forearm.reconfigure TightForearm @02:02:08.26 Forearm.seek CC @02:02:09.65 CC.seek :closed @02:02:13.98 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.42 @02:02:14.07 Hand.open @02:02:14.85 Forearm.reconfigure LooseForearm @02:02:14.90 Forearm.seek :retract @02:02:15.93 Flushing the Collection Valve::Manifold @02:02:16.07 CSV.dial CSR @02:02:16.64 Pulling 5ml of CSR.flush CSR.select :flush @02:02:16.76 CS.reconfigure CSconfig @02:02:16.81 CS.seek 5ml @02:02:30.93 delay 5 seconds @02:02:35.95 CSR.select :air @02:02:36.13 CS.reconfigure CSfast @02:02:36.18 CS.seek 7.5ml @02:02:43.50 CSV.dial :waste @02:02:45.05 CS.reconfigure CSconfig @02:02:45.10 CS.seek 0ml @02:03:05.47 Purging external Intake @02:03:05.61 CSV.dial :flush @02:03:06.13 CS.reconfigure CSfast @02:03:06.18 CS.seek 10ml @02:03:33.00 delay 5 seconds @02:03:38.02 CSV.dial CBV @02:03:38.06
CBV.dial CSV @02:03:39.00 CTV.dial :intake @02:03:39.86 Intake.select :open @02:03:39.98 CS.seek 0ml @02:04:07.53 Intake.select :close @02:04:07.64 CTV.dial :air @02:04:08.51 Flushing upper manifold @02:04:08.66 CSV.dial :flush @02:04:09.51 CS.seek 5ml @02:04:23.28 delay 5 seconds @02:04:28.30 CSV.dial :air @02:04:28.91 CS.seek 7ml @02:04:35.00 CSV.dial CBV @02:04:35.03
CBV.dial CSV @02:04:36.36 CTV.dial :air @02:04:36.39 CS.seek 0ml @02:04:55.25 delay 7 seconds @02:05:02.28 CS.seek 3ml @02:05:11.03 CSV.dial :waste @02:05:11.54 CS.seek 0ml @02:05:20.31 CSV.dial :flush @02:05:20.84 CS.seek 3ml @02:05:29.53 delay 5 seconds @02:05:34.55 CSV.dial :air @02:05:35.13 CS.seek 5ml @02:05:41.16 CSV.dial :air @02:05:41.18 CS.seek 0ml @02:05:54.81 delay 7 seconds @02:06:01.84 CS.seek 3ml @02:06:10.59 CSV.dial :waste @02:06:11.46 CS.seek 0ml @02:06:20.06 Garaging FlushPuck in Collection Clamp @02:06:20.14 Elbow.reconfigure TightElbow @02:06:20.19 Elbow.seek CC @02:06:20.43 Elbow.reconfigure LooseElbow @02:06:20.53 Forearm.seek CC @02:06:21.61 Hand.close @02:06:22.42 CC.seek :open @02:06:26.85 Forearm.seek :retract @02:06:27.89 Elbow.reconfigure TightElbow @02:06:27.94 Elbow.seek :garage @02:06:31.19 Elbow.reconfigure LooseElbow @02:06:31.29 Forearm.reconfigure TightForearm @02:06:31.34 Forearm.seek :garage @02:06:32.53 Hand.open @02:06:33.35 Forearm.reconfigure LooseForearm @02:06:33.40 Forearm.seek :retract @02:06:34.45 Completed [mbaPhase] begun 22:50:33 @02:06:34.47 Extending 190523totalPostcanqc until completion of MFBPCR @03:03:51.28 PCR.off @03:03:53.89 PCR for IPc completed -- closed /var/log/esp/IPc19may2401h1000ml.pcr @03:03:53.96 Cleaning PCR @03:03:53.97 AFV.select :carrierSolution @03:03:54.07 delay 2 seconds @03:03:56.11 AS.reconfigure ASconfig @03:03:56.15 AS.seek 0.5ml @03:04:06.46 delay 1 second @03:04:07.48 AFV.select :holdingCoil @03:04:07.58 ASV.dial :waste1 @03:04:07.98 ATV.dial :air3 @03:04:09.30 AS.reconfigure ASslow1 @03:04:09.35 AS.seek 0.51ml @03:04:11.66 delay 2 seconds @03:04:13.68 ATV.dial :bleach @03:04:14.84 AS.seek 0.81ml @03:05:15.45 delay 3 seconds @03:05:18.48 ATV.dial :air3 @03:05:19.04 delay 3 seconds @03:05:22.13 AS.seek 0.835ml @03:05:27.44 delay 3 seconds @03:05:30.46 ATV.dial :bleach @03:05:31.10 AS.seek 0.82ml @03:05:34.34 delay 3 seconds @03:05:37.36 ARV.dial :waste @03:05:37.98 delay 3 seconds @03:05:41.01 ATV.dial :ARV @03:05:41.92 AS.reconfigure ASconfig @03:05:41.97 AS.seek 0.47ml @03:06:02.38 AFV.select :carrierSolution @03:06:02.49 delay 2 seconds @03:06:04.53 AS.seek 1ml @03:06:35.27 delay 1 second @03:06:36.29 AFV.select :holdingCoil @03:06:36.39 delay 1 second @03:06:37.43 AS.reconfigure ASfast @03:06:37.47 AS.seek 0ml @03:07:04.82 AFV.select :carrierSolution @03:07:04.93 delay 2 seconds @03:07:06.97 AS.reconfigure ASconfig @03:07:07.02 AS.seek 1ml @03:08:04.76 delay 1 second @03:08:05.78 AFV.select :holdingCoil @03:08:05.88 delay 1 second @03:08:06.92 AS.reconfigure ASfast @03:08:06.96 AS.seek 0ml @03:08:34.32 AFV.select :carrierSolution @03:08:34.43 delay 2 seconds @03:08:36.47 AS.reconfigure ASconfig @03:08:36.52 AS.seek 1ml @03:09:34.34 delay 1 second @03:09:35.36 AFV.select :holdingCoil @03:09:35.46 delay 1 second @03:09:36.50 AS.reconfigure ASfast @03:09:36.54 AS.seek 0ml @03:10:03.90 ARV.dial between carrier and air1 @03:10:04.41 ATV.dial :air3 @03:10:05.72 AS.reconfigure ASslow1 @03:10:05.78 AS.seek 0.01ml @03:10:08.10 delay 2 seconds @03:10:10.12 ATV.dial :bleach @03:10:10.78 AS.seek 0.81ml @03:12:51.91 delay 3 seconds @03:12:54.94 ATV.dial :air3 @03:12:55.51 delay 3 seconds @03:12:58.60 AS.seek 0.835ml @03:13:03.90 delay 3 seconds @03:13:06.92 ATV.dial :bleach @03:13:07.56 AS.seek 0.82ml @03:13:10.85 delay 3 seconds @03:13:13.87 ATV.dial PCR @03:13:14.28 AS.reconfigure ASconfig @03:13:14.32 AS.seek :empty @03:14:01.79 PCR.program :bleachProfile @03:14:01.84 PCR program should complete in < 2:45 @03:14:01.85 PCR.run 10:00 @03:17:03.65 PCR.off @03:17:03.68 AFV.select :carrierSolution @03:17:03.78 delay 2 seconds @03:17:05.82 AS.seek 1ml @03:18:03.60 delay 1 second @03:18:04.62 AFV.select :holdingCoil @03:18:04.72 delay 1 second @03:18:05.76 AS.reconfigure ASfast @03:18:05.80 AS.seek 0ml @03:18:33.15 delay 3 seconds @03:18:36.18 AFV.select :carrierSolution @03:18:36.28 delay 2 seconds @03:18:38.32 AS.reconfigure ASconfig @03:18:38.37 AS.seek 1ml @03:19:36.15 delay 1 second @03:19:37.17 AFV.select :holdingCoil @03:19:37.27 delay 1 second @03:19:38.31 AS.reconfigure ASfast @03:19:38.35 AS.seek 0ml @03:20:05.70 delay 3 seconds @03:20:08.73 AFV.select :carrierSolution @03:20:08.83 delay 2 seconds @03:20:10.87 AS.reconfigure ASconfig @03:20:10.92 AS.seek 1ml @03:21:08.66 delay 1 second @03:21:09.68 AFV.select :holdingCoil @03:21:09.78 delay 1 second @03:21:10.82 AS.reconfigure ASfast @03:21:10.86 AS.seek 0ml @03:21:38.20 delay 3 seconds @03:21:41.22 AFV.select :carrierSolution @03:21:41.32 delay 2 seconds @03:21:43.86 AS.reconfigure ASconfig @03:21:43.98 AS.seek 1ml @03:22:41.73 delay 1 second @03:22:42.75 AFV.select :holdingCoil @03:22:42.85 delay 1 second @03:22:43.89 AS.reconfigure ASfast @03:22:43.93 AS.seek 0ml @03:23:11.29 delay 3 seconds @03:23:14.32 AFV.select :carrierSolution @03:23:14.42 delay 2 seconds @03:23:16.46 AS.reconfigure ASconfig @03:23:16.51 AS.seek 1ml @03:24:14.29 delay 1 second @03:24:15.31 AFV.select :holdingCoil @03:24:15.41 delay 1 second @03:24:16.45 AS.reconfigure ASnorm @03:24:16.49 AS.seek 0ml @03:25:07.23 Setting up PCR for Pp -- writing /var/log/esp/Pp19may2403h1000ml.pcr @03:25:07.25 Building PCR Slug from ASV with MM1 mix, PP2 primer @03:25:07.26 Priming with carrier @03:25:07.27 ATV.dial ARV @03:25:07.90 ARV.dial :carrier @03:25:08.28 AS.seek 0.5ml @03:25:33.85 AFV.select :carrierSolution @03:25:33.96 delay 2 seconds @03:25:36.00 AS.reconfigure ASconfig @03:25:36.04 AS.seek 1ml @03:26:05.09 delay 1 second @03:26:06.11 AFV.select :holdingCoil @03:26:06.21 delay 1 second @03:26:07.24 ATV.dial :waste @03:26:08.94 AS.reconfigure ASnorm @03:26:08.99 AS.seek :empty @03:26:59.73 AFV.select :carrierSolution @03:26:59.84 delay 2 seconds @03:27:01.88 AS.reconfigure ASconfig @03:27:01.92 AS.seek 0.5ml @03:27:31.02 delay 1 second @03:27:32.04 AFV.select :holdingCoil @03:27:32.15 Building Slug @03:27:32.16 ATV.dial :ARV @03:27:33.82 delay 2 seconds @03:27:35.85 ARV.dial :air1 @03:27:36.25 delay 2 seconds @03:27:38.34 AS.reconfigure ASslow2 @03:27:38.39 AS.seek 0.506ml @03:27:44.93 delay 2 seconds @03:27:46.96 ARV.dial :MM1 @03:27:47.56 delay 2 seconds @03:27:49.60 Adding MM1 @03:27:49.67 AS.seek 0.524ml @03:28:07.90 delay 25 seconds @03:28:32.93 ARV.dial :PP2 @03:28:33.33 Adding PP2 @03:28:33.34 delay 2 seconds @03:28:35.42 AS.seek 0.53ml @03:28:41.60 delay 10 seconds @03:28:51.63 ARV.dial :air2 @03:28:52.03 adding ARV air @03:28:52.04 delay 2 seconds @03:28:54.12 AS.seek 0.536ml @03:29:00.49 delay 5 seconds @03:29:05.52 ARV.dial :carrier @03:29:06.82 delay 7 seconds @03:29:13.91 AS.seek 0.594ml @03:30:12.59 delay 5 seconds @03:30:17.62 ATV.dial :ASV @03:30:18.02 delay 2 seconds @03:30:20.11 AS.seek 0.6ml @03:30:26.62 delay 4 seconds @03:30:30.65 ATV.dial ARV @03:30:31.20 delay 2 seconds @03:30:33.23 ARV.dial :carrier @03:30:33.25 delay 2 seconds @03:30:35.34 AS.seek 0.65ml @03:31:26.01 delay 4 seconds @03:31:30.04 ATV.dial PCR @03:31:30.65 ASV.dial :waste1 @03:31:30.67 ARV.dial :air1 @03:31:31.09 Positioning Slug by 0.324ml @03:31:31.17 AS.seek 0.326ml @03:36:57.27 delay 5 seconds @03:37:02.30 ATV.dial ARV @03:37:03.09 ASV.dial :PCRlock @03:37:03.49 delay 5 seconds @03:37:08.53 Running Pp Profile60C350 thermocycling @03:37:08.54 PCR.program Profile60C350 @03:37:08.68 Profile60C350 should complete in < 1:48:05 @03:37:08.70 PCR.run 2:20:06.25 @05:01:54.43 PCR.off @05:01:57.02 PCR for Pp completed -- closed /var/log/esp/Pp19may2403h1000ml.pcr @05:01:57.09 Cleaning PCR @05:01:57.10 AFV.select :carrierSolution @05:01:57.21 delay 2 seconds @05:01:59.25 AS.reconfigure ASconfig @05:01:59.29 AS.seek 0.5ml @05:02:09.60 delay 1 second @05:02:10.62 AFV.select :holdingCoil @05:02:10.72 ASV.dial :waste1 @05:02:11.12 ATV.dial :air3 @05:02:12.44 AS.reconfigure ASslow1 @05:02:12.49 AS.seek 0.51ml @05:02:14.76 delay 2 seconds @05:02:16.78 ATV.dial :bleach @05:02:17.42 AS.seek 0.81ml @05:03:18.12 delay 3 seconds @05:03:21.15 ATV.dial :air3 @05:03:21.70 delay 3 seconds @05:03:24.79 AS.seek 0.835ml @05:03:30.12 delay 3 seconds @05:03:33.14 ATV.dial :bleach @05:03:33.78 AS.seek 0.82ml @05:03:37.09 delay 3 seconds @05:03:40.11 ARV.dial :waste @05:03:40.74 delay 3 seconds @05:03:43.77 ATV.dial :ARV @05:03:44.78 AS.reconfigure ASconfig @05:03:44.83 AS.seek 0.47ml @05:04:05.23 AFV.select :carrierSolution @05:04:05.34 delay 2 seconds @05:04:07.38 AS.seek 1ml @05:04:38.15 delay 1 second @05:04:39.17 AFV.select :holdingCoil @05:04:39.27 delay 1 second @05:04:40.31 AS.reconfigure ASfast @05:04:40.36 AS.seek 0ml @05:05:07.71 AFV.select :carrierSolution @05:05:07.82 delay 2 seconds @05:05:09.86 AS.reconfigure ASconfig @05:05:09.90 AS.seek 1ml @05:06:07.68 delay 1 second @05:06:08.70 AFV.select :holdingCoil @05:06:08.80 delay 1 second @05:06:09.84 AS.reconfigure ASfast @05:06:09.88 AS.seek 0ml @05:06:37.24 AFV.select :carrierSolution @05:06:37.35 delay 2 seconds @05:06:39.39 AS.reconfigure ASconfig @05:06:39.43 AS.seek 1ml @05:07:37.23 delay 1 second @05:07:38.25 AFV.select :holdingCoil @05:07:38.35 delay 1 second @05:07:39.39 AS.reconfigure ASfast @05:07:39.43 AS.seek 0ml @05:08:06.79 ARV.dial between carrier and air1 @05:08:07.31 ATV.dial :air3 @05:08:08.63 AS.reconfigure ASslow1 @05:08:08.68 AS.seek 0.01ml @05:08:10.99 delay 2 seconds @05:08:13.01 ATV.dial :bleach @05:08:13.64 AS.seek 0.81ml @05:10:54.77 delay 3 seconds @05:10:57.79 ATV.dial :air3 @05:10:58.34 delay 3 seconds @05:11:01.43 AS.seek 0.835ml @05:11:06.71 delay 3 seconds @05:11:09.74 ATV.dial :bleach @05:11:10.38 AS.seek 0.82ml @05:11:13.81 delay 3 seconds @05:11:16.83 ATV.dial PCR @05:11:17.25 AS.reconfigure ASconfig @05:11:17.29 AS.seek :empty @05:12:04.77 PCR.program :bleachProfile @05:12:04.82 PCR program should complete in < 2:45 @05:12:04.83 PCR.run 10:00 @05:15:06.64 PCR.off @05:15:06.67 AFV.select :carrierSolution @05:15:06.78 delay 2 seconds @05:15:08.82 AS.seek 1ml @05:16:06.65 delay 1 second @05:16:07.67 AFV.select :holdingCoil @05:16:07.77 delay 1 second @05:16:08.81 AS.reconfigure ASfast @05:16:08.85 AS.seek 0ml @05:16:36.21 delay 3 seconds @05:16:39.23 AFV.select :carrierSolution @05:16:39.33 delay 2 seconds @05:16:41.37 AS.reconfigure ASconfig @05:16:41.42 AS.seek 1ml @05:17:39.78 delay 1 second @05:17:40.81 AFV.select :holdingCoil @05:17:40.91 delay 1 second @05:17:41.95 AS.reconfigure ASfast @05:17:41.99 AS.seek 0ml @05:18:09.37 delay 3 seconds @05:18:12.40 AFV.select :carrierSolution @05:18:12.50 delay 2 seconds @05:18:14.54 AS.reconfigure ASconfig @05:18:14.59 AS.seek 1ml @05:19:12.34 delay 1 second @05:19:13.36 AFV.select :holdingCoil @05:19:13.46 delay 1 second @05:19:14.50 AS.reconfigure ASfast @05:19:14.54 AS.seek 0ml @05:19:41.91 delay 3 seconds @05:19:44.94 AFV.select :carrierSolution @05:19:45.04 delay 2 seconds @05:19:47.08 AS.reconfigure ASconfig @05:19:47.13 AS.seek 1ml @05:20:44.88 delay 1 second @05:20:45.90 AFV.select :holdingCoil @05:20:46.00 delay 1 second @05:20:47.04 AS.reconfigure ASfast @05:20:47.08 AS.seek 0ml @05:21:14.43 delay 3 seconds @05:21:17.46 AFV.select :carrierSolution @05:21:17.56 delay 2 seconds @05:21:19.60 AS.reconfigure ASconfig @05:21:19.66 AS.seek 1ml @05:22:17.43 delay 1 second @05:22:18.45 AFV.select :holdingCoil @05:22:18.55 delay 1 second @05:22:19.59 AS.reconfigure ASnorm @05:22:19.63 AS.seek 0ml @05:23:10.37 Setting up PCR for Ss -- writing /var/log/esp/Ss19may2405h1000ml.pcr @05:23:10.39 Building PCR Slug from ASV with MM2 mix, PP4 primer @05:23:10.40 Priming with carrier @05:23:10.41 ATV.dial ARV @05:23:11.02 ARV.dial :carrier @05:23:11.38 AS.seek 0.5ml @05:23:36.96 AFV.select :carrierSolution @05:23:37.07 delay 2 seconds @05:23:39.11 AS.reconfigure ASconfig @05:23:39.15 AS.seek 1ml @05:24:08.16 delay 1 second @05:24:09.18 AFV.select :holdingCoil @05:24:09.29 delay 1 second @05:24:10.31 ATV.dial :waste @05:24:12.02 AS.reconfigure ASnorm @05:24:12.07 AS.seek :empty @05:25:02.81 AFV.select :carrierSolution @05:25:02.92 delay 2 seconds @05:25:04.96 AS.reconfigure ASconfig @05:25:05.01 AS.seek 0.5ml @05:25:34.88 delay 1 second @05:25:35.90 AFV.select :holdingCoil @05:25:36.00 Building Slug @05:25:36.02 ATV.dial :ARV @05:25:37.68 delay 2 seconds @05:25:39.71 ARV.dial :air1 @05:25:40.11 delay 2 seconds @05:25:42.20 AS.reconfigure ASslow2 @05:25:42.25 AS.seek 0.506ml @05:25:48.73 delay 2 seconds @05:25:50.75 ARV.dial :MM2 @05:25:52.26 delay 2 seconds @05:25:54.29 Adding MM2 @05:25:54.38 AS.seek 0.524ml @05:26:12.68 delay 25 seconds @05:26:37.71 ARV.dial :PP4 @05:26:38.10 Adding PP4 @05:26:38.12 delay 2 seconds @05:26:40.20 AS.seek 0.53ml @05:26:46.38 delay 10 seconds @05:26:56.41 ARV.dial :air3 @05:26:56.82 adding ARV air @05:26:56.83 delay 2 seconds @05:26:58.91 AS.seek 0.536ml @05:27:05.46 delay 5 seconds @05:27:10.49 ARV.dial :carrier @05:27:11.77 delay 7 seconds @05:27:18.86 AS.seek 0.594ml @05:28:17.43 delay 5 seconds @05:28:22.46 ATV.dial :ASV @05:28:22.88 delay 2 seconds @05:28:24.97 AS.seek 0.6ml @05:28:31.35 delay 4 seconds @05:28:35.38 ATV.dial ARV @05:28:35.93 delay 2 seconds @05:28:37.96 ARV.dial :carrier @05:28:37.98 delay 2 seconds @05:28:40.06 AS.seek 0.65ml @05:29:30.73 delay 4 seconds @05:29:34.76 ATV.dial PCR @05:29:35.38 ASV.dial :waste1 @05:29:35.40 ARV.dial :air1 @05:29:35.79 Positioning Slug by 0.324ml @05:29:35.87 AS.seek 0.326ml @05:33:13.33 Session Begun @05:33:40.26 -> GoodNews.email "Test for upload trigger" @05:33:40.28 GoodNews.email "Test for upload trigger" @05:35:02.02 delay 5 seconds @05:35:07.05 ATV.dial ARV @05:35:07.96 ASV.dial :PCRlock @05:35:08.40 delay 5 seconds @05:35:13.43 Running Ss Profile60C350 thermocycling @05:35:13.45 PCR.program Profile60C350 @05:35:13.62 Profile60C350 should complete in < 1:48:05 @05:35:13.63 PCR.run 2:20:06.25 @06:26:15.70 -> GoodNews.email "Another test upload trigger email" @06:26:15.72 GoodNews.email "Another test upload trigger email" @06:34:43.83 -> Time.now @06:34:51.86 -> exit @06:34:51.88 Session Ended @06:59:34.47 PCR.off @06:59:36.55 PCR for Ss completed -- closed /var/log/esp/Ss19may2405h1000ml.pcr @06:59:36.62 Cleaning PCR @06:59:36.63 AFV.select :carrierSolution @06:59:36.74 delay 2 seconds @06:59:38.78 AS.reconfigure ASconfig @06:59:38.82 AS.seek 0.5ml @06:59:49.17 delay 1 second @06:59:50.19 AFV.select :holdingCoil @06:59:50.29 ASV.dial :waste1 @06:59:50.70 ATV.dial :air3 @06:59:52.02 AS.reconfigure ASslow1 @06:59:52.07 AS.seek 0.51ml @06:59:54.39 delay 2 seconds @06:59:56.41 ATV.dial :bleach @06:59:57.05 AS.seek 0.81ml @07:00:57.63 delay 3 seconds @07:01:00.66 ATV.dial :air3 @07:01:01.21 delay 3 seconds @07:01:04.30 AS.seek 0.835ml @07:01:09.63 delay 3 seconds @07:01:12.66 ATV.dial :bleach @07:01:13.28 AS.seek 0.82ml @07:01:16.59 delay 3 seconds @07:01:19.61 ARV.dial :waste @07:01:20.23 delay 3 seconds @07:01:23.26 ATV.dial :ARV @07:01:24.17 AS.reconfigure ASconfig @07:01:24.22 AS.seek 0.47ml @07:01:44.65 AFV.select :carrierSolution @07:01:44.76 delay 2 seconds @07:01:46.80 AS.seek 1ml @07:02:17.54 delay 1 second @07:02:18.56 AFV.select :holdingCoil @07:02:18.66 delay 1 second @07:02:19.70 AS.reconfigure ASfast @07:02:19.74 AS.seek 0ml @07:02:47.10 AFV.select :carrierSolution @07:02:47.21 delay 2 seconds @07:02:49.25 AS.reconfigure ASconfig @07:02:49.29 AS.seek 1ml @07:03:47.06 delay 1 second @07:03:48.08 AFV.select :holdingCoil @07:03:48.18 delay 1 second @07:03:49.22 AS.reconfigure ASfast @07:03:49.26 AS.seek 0ml @07:04:16.62 AFV.select :carrierSolution @07:04:16.73 delay 2 seconds @07:04:18.77 AS.reconfigure ASconfig @07:04:18.81 AS.seek 1ml @07:05:16.60 delay 1 second @07:05:17.62 AFV.select :holdingCoil @07:05:17.72 delay 1 second @07:05:18.76 AS.reconfigure ASfast @07:05:18.80 AS.seek 0ml @07:05:46.13 ARV.dial between carrier and air1 @07:05:46.63 ATV.dial :air3 @07:05:47.94 AS.reconfigure ASslow1 @07:05:48.55 AS.seek 0.01ml @07:05:50.90 delay 2 seconds @07:05:52.92 ATV.dial :bleach @07:05:53.56 AS.seek 0.81ml @07:08:34.79 delay 3 seconds @07:08:37.82 ATV.dial :air3 @07:08:38.37 delay 3 seconds @07:08:41.46 AS.seek 0.835ml @07:08:46.76 delay 3 seconds @07:08:49.79 ATV.dial :bleach @07:08:50.41 AS.seek 0.82ml @07:08:53.68 delay 3 seconds @07:08:56.70 ATV.dial PCR @07:08:57.13 AS.reconfigure ASconfig @07:08:57.18 AS.seek :empty @07:09:44.63 PCR.program :bleachProfile @07:09:44.68 PCR program should complete in < 2:45 @07:09:44.69 PCR.run 10:00 @07:12:46.56 PCR.off @07:12:46.59 AFV.select :carrierSolution @07:12:46.70 delay 2 seconds @07:12:48.74 AS.seek 1ml @07:13:46.54 delay 1 second @07:13:47.56 AFV.select :holdingCoil @07:13:47.66 delay 1 second @07:13:48.70 AS.reconfigure ASfast @07:13:48.74 AS.seek 0ml @07:14:16.12 delay 3 seconds @07:14:19.14 AFV.select :carrierSolution @07:14:19.29 delay 2 seconds @07:14:21.34 AS.reconfigure ASconfig @07:14:21.41 AS.seek 1ml @07:15:19.20 delay 1 second @07:15:20.23 AFV.select :holdingCoil @07:15:20.36 delay 1 second @07:15:21.42 AS.reconfigure ASfast @07:15:21.50 AS.seek 0ml @07:15:48.89 delay 3 seconds @07:15:51.93 AFV.select :carrierSolution @07:15:52.06 delay 2 seconds @07:15:54.13 AS.reconfigure ASconfig @07:15:54.21 AS.seek 1ml @07:16:52.04 delay 1 second @07:16:53.07 AFV.select :holdingCoil @07:16:53.20 delay 1 second @07:16:54.26 AS.reconfigure ASfast @07:16:54.33 AS.seek 0ml @07:17:21.72 delay 3 seconds @07:17:24.76 AFV.select :carrierSolution @07:17:24.89 delay 2 seconds @07:17:26.96 AS.reconfigure ASconfig @07:17:27.03 AS.seek 1ml @07:18:24.83 delay 1 second @07:18:25.86 AFV.select :holdingCoil @07:18:25.99 delay 1 second @07:18:27.05 AS.reconfigure ASfast @07:18:27.12 AS.seek 0ml @07:18:54.55 delay 3 seconds @07:18:57.58 AFV.select :carrierSolution @07:18:57.71 delay 2 seconds @07:18:59.77 AS.reconfigure ASconfig @07:18:59.84 AS.seek 1ml @07:19:57.64 delay 1 second @07:19:58.67 AFV.select :holdingCoil @07:19:58.82 delay 1 second @07:19:59.88 AS.reconfigure ASnorm @07:19:59.95 AS.seek 0ml @07:20:50.76 Setting up PCR for Mm -- writing /var/log/esp/Mm19may2407h1000ml.pcr @07:20:50.80 Building PCR Slug from ASV with MM2 mix, PP3 primer @07:20:50.82 Priming with carrier @07:20:50.84 ATV.dial ARV @07:20:51.51 ARV.dial :carrier @07:20:52.04 AS.seek 0.5ml @07:21:17.65 AFV.select :carrierSolution @07:21:17.80 delay 2 seconds @07:21:19.87 AS.reconfigure ASconfig @07:21:19.95 AS.seek 1ml @07:21:49.01 delay 1 second @07:21:50.04 AFV.select :holdingCoil @07:21:50.17 delay 1 second @07:21:51.21 ATV.dial :waste @07:21:52.99 AS.reconfigure ASnorm @07:21:53.11 AS.seek :empty @07:22:43.89 AFV.select :carrierSolution @07:22:44.03 delay 2 seconds @07:22:46.09 AS.reconfigure ASconfig @07:22:46.19 AS.seek 0.5ml @07:23:15.35 delay 1 second @07:23:16.38 AFV.select :holdingCoil @07:23:16.53 Building Slug @07:23:16.55 ATV.dial :ARV @07:23:18.26 delay 2 seconds @07:23:20.30 ARV.dial :air1 @07:23:20.75 delay 2 seconds @07:23:22.91 AS.reconfigure ASslow2 @07:23:23.01 AS.seek 0.506ml @07:23:29.23 delay 2 seconds @07:23:31.26 ARV.dial :MM2 @07:23:32.81 delay 2 seconds @07:23:34.86 Adding MM2 @07:23:36.24 AS.seek 0.524ml @07:23:54.58 delay 25 seconds @07:24:19.62 ARV.dial :PP3 @07:24:20.10 Adding PP3 @07:24:20.12 delay 2 seconds @07:24:22.27 AS.seek 0.53ml @07:24:28.50 delay 10 seconds @07:24:38.54 ARV.dial :air2 @07:24:38.99 adding ARV air @07:24:39.02 delay 2 seconds @07:24:41.17 AS.seek 0.536ml @07:24:47.44 delay 5 seconds @07:24:52.48 ARV.dial :carrier @07:24:53.83 delay 7 seconds @07:25:01.00 AS.seek 0.594ml @07:25:59.51 delay 5 seconds @07:26:04.55 ATV.dial :ASV @07:26:05.00 delay 2 seconds @07:26:07.19 AS.seek 0.6ml @07:26:13.42 delay 4 seconds @07:26:17.46 ATV.dial ARV @07:26:18.06 delay 2 seconds @07:26:20.11 ARV.dial :carrier @07:26:20.15 delay 2 seconds @07:26:22.31 AS.seek 0.65ml @07:27:13.06 delay 4 seconds @07:27:17.10 ATV.dial PCR @07:27:17.78 ASV.dial :waste1 @07:27:17.82 ARV.dial :air1 @07:27:18.27 Positioning Slug by 0.324ml @07:27:18.43 AS.seek 0.326ml @07:32:44.80 delay 5 seconds @07:32:49.83 ATV.dial ARV @07:32:50.67 ASV.dial :PCRlock @07:32:51.12 delay 5 seconds @07:32:56.17 Running Mm Profile60C350 thermocycling @07:32:56.21 PCR.program Profile60C350 @07:32:56.40 Profile60C350 should complete in < 1:48:05 @07:32:56.44 PCR.run 2:20:06.25 @08:26:05.07 Session Begun @08:26:10.63 -> showlog 10 @08:26:37.65 -> showlog 100 @08:27:04.98 -> exit @08:27:05.00 Session Ended @08:57:27.33 PCR.off @08:57:29.39 PCR for Mm completed -- closed /var/log/esp/Mm19may2407h1000ml.pcr @08:57:29.40 Full MFB Decontamination @08:57:29.41 Decon extraction column and decon AIV @08:57:29.44 SPE.hold 2:46:40,55C @08:57:29.48 ASV.dial :SPEdelivery @08:57:30.15 delay 20 seconds @08:57:50.18 AFV.select :carrierSolution @08:57:50.28 delay 2 seconds @08:57:52.32 AS.reconfigure ASconfig @08:57:52.36 AS.seek 0.5ml @08:58:02.63 delay 1 second @08:58:03.65 AFV.select :holdingCoil @08:58:03.75 ATV.dial :air1 @08:58:05.78 AS.reconfigure ASslow1 @08:58:05.83 AS.seek 0.52ml @08:58:10.13 ATV.dial :ASV @08:58:11.99 ASV.dial :SPErecovery @08:58:12.67 AS.seek 0.62ml @08:58:33.18 ATV.dial :waste @08:58:35.31 AS.reconfigure ASnorm @08:58:35.35 AS.seek :empty @08:59:06.96 AFV.select :holdingCoil @08:59:06.97 ATV.dial :waste @08:59:06.99 AS.reconfigure ASconfig @08:59:07.04 AS.seek 0ml @08:59:07.32 AFV.select :carrierSolution @08:59:07.43 delay 2 seconds @08:59:09.47 AS.seek 0.5ml @08:59:38.49 delay 1 second @08:59:39.51 AFV.select :holdingCoil @08:59:39.61 ATV.dial :air3 @08:59:40.37 AS.reconfigure ASslow1 @08:59:40.43 AS.seek 0.52ml @08:59:44.74 ATV.dial :air3 @08:59:44.83 AS.seek 0.53ml @08:59:47.04 delay 2 seconds @08:59:49.06 ATV.dial :bleach @08:59:49.56 AS.seek 0.71ml @09:00:26.05 delay 3 seconds @09:00:29.07 ATV.dial :air3 @09:00:29.66 delay 3 seconds @09:00:32.75 AS.seek 0.735ml @09:00:38.13 delay 3 seconds @09:00:41.15 ATV.dial :bleach @09:00:41.80 AS.seek 0.72ml @09:00:45.68 delay 3 seconds @09:00:48.70 ATV.dial ASV @09:00:49.82 ASV.dial :SPErecovery @09:00:49.91 AS.seek 0.365ml @09:02:01.05 Session Begun @09:02:01.57 ATV.dial :waste @09:02:03.39 AS.reconfigure ASnorm @09:02:03.46 AS.seek :empty @09:02:06.66 -> exit @09:02:06.68 Session Ended @09:02:22.23 ATV.dial :air3 @09:02:22.94 AS.reconfigure ASslow1 @09:02:23.00 AS.seek 0.009ml @09:02:25.40 delay 2 seconds @09:02:27.42 ATV.dial :bleach @09:02:27.91 AS.seek 0.579ml @09:04:22.84 delay 3 seconds @09:04:25.87 ATV.dial :air3 @09:04:26.44 delay 3 seconds @09:04:29.53 AS.seek 0.604ml @09:04:34.82 delay 3 seconds @09:04:37.84 ATV.dial :bleach @09:04:38.47 AS.seek 0.589ml @09:04:41.76 delay 3 seconds @09:04:44.79 ATV.dial :ASV @09:04:45.85 ASV.dial :wasteR @09:04:46.33 AS.seek 0ml @09:06:45.10 delay 1:00 @09:07:45.13 ATV.dial ASV @09:07:45.14 AFV.select :carrierSolution @09:07:45.24 delay 2 seconds @09:07:47.28 AS.reconfigure ASconfig @09:07:47.32 AS.seek 1ml @09:08:45.10 delay 1 second @09:08:46.12 AFV.select :holdingCoil @09:08:46.22 delay 1 second @09:08:47.25 ASV.dial :wasteR @09:08:47.27 AS.reconfigure ASslow1 @09:08:47.31 AS.seek 0ml @09:12:08.71 delay 10 seconds @09:12:18.74 ATV.dial :air3 @09:12:20.28 AS.seek 0.01ml @09:12:22.73 delay 2 seconds @09:12:24.75 ATV.dial :bleach @09:12:25.39 AS.seek 0.58ml @09:14:20.41 delay 3 seconds @09:14:23.44 ATV.dial :air3 @09:14:23.99 delay 3 seconds @09:14:27.08 AS.seek 0.605ml @09:14:32.41 delay 3 seconds @09:14:35.43 ATV.dial :bleach @09:14:36.06 AS.seek 0.59ml @09:14:39.37 delay 3 seconds @09:14:42.40 ATV.dial ASV @09:14:43.46 ASV.dial :SPEdelivery @09:14:43.85 AS.seek 0ml @09:16:42.74 delay 1:00 @09:17:42.77 AFV.select :carrierSolution @09:17:42.87 delay 2 seconds @09:17:44.91 AS.reconfigure ASconfig @09:17:44.95 AS.seek 1ml @09:18:42.74 delay 1 second @09:18:43.76 AFV.select :holdingCoil @09:18:43.86 delay 1 second @09:18:44.89 ATV.dial ASV @09:18:44.90 ASV.dial :SPEdelivery @09:18:44.92 AS.reconfigure ASslow1 @09:18:44.97 AS.seek 0ml @09:22:06.37 delay 10 seconds @09:22:16.40 ATV.dial :air2 @09:22:17.25 AS.reconfigure ASslow0 @09:22:17.30 AS.seek 0.8ml @09:23:38.05 delay 3 seconds @09:23:41.08 ATV.dial :ASV @09:23:42.10 ASV.dial :SPEdelivery @09:23:42.12 AS.reconfigure ASslow1 @09:23:42.17 AS.seek 0.03ml @09:26:17.49 delay 20 seconds @09:26:37.52 ATV.dial :waste,via: :air3 @09:26:39.30 AS.reconfigure ASconfig @09:26:39.34 AS.seek :empty @09:26:41.44 SPE.coast @09:26:41.53 Removing Bleach from Mixing Coil @09:26:41.54 AFV.select :carrierSolution @09:26:41.64 delay 2 seconds @09:26:43.68 AS.seek 0.5ml @09:27:12.74 delay 1 second @09:27:13.76 AFV.select :holdingCoil @09:27:13.86 ATV.dial :ASV @09:27:15.59 ASV.dial :SPErecovery @09:27:16.25 AS.reconfigure ASslow1 @09:27:16.30 AS.seek 0.73ml @09:28:02.80 ASV.dial :wasteD @09:28:03.97 AS.reconfigure ASnorm @09:28:04.02 AS.seek 0ml @09:28:41.13 AFV.select :carrierSolution @09:28:41.24 delay 2 seconds @09:28:43.28 AS.reconfigure ASconfig @09:28:43.32 AS.seek 0.5ml @09:29:12.40 delay 1 second @09:29:13.42 AFV.select :holdingCoil @09:29:13.52 ATV.dial ASV @09:29:13.54 ASV.dial :SPErecovery @09:29:14.19 AS.reconfigure ASslow0 @09:29:14.24 AS.seek 0.3ml @09:29:34.69 ATV.dial :air2 @09:29:35.75 AS.reconfigure ASslow1 @09:29:35.82 AS.seek 0.32ml @09:29:40.14 ATV.dial ASV @09:29:41.20 AS.reconfigure ASslow0 @09:29:41.25 AS.seek 0.57ml @09:30:06.74 ASV.dial :wasteD @09:30:07.33 AS.reconfigure ASnorm @09:30:07.38 AS.seek 0ml @09:30:36.46 AFV.select :carrierSolution @09:30:36.57 delay 2 seconds @09:30:38.61 AS.reconfigure ASconfig @09:30:38.67 AS.seek 0.5ml @09:31:07.74 delay 1 second @09:31:08.76 AFV.select :holdingCoil @09:31:08.86 ATV.dial ASV @09:31:08.88 ASV.dial :SPErecovery @09:31:09.53 AS.reconfigure ASslow0 @09:31:09.58 AS.seek 0.3ml @09:31:30.04 ATV.dial :air2 @09:31:31.11 AS.reconfigure ASslow1 @09:31:31.16 AS.seek 0.32ml @09:31:35.49 ATV.dial ASV @09:31:36.58 AS.reconfigure ASslow0 @09:31:36.63 AS.seek 0.57ml @09:32:02.16 ASV.dial :wasteD @09:32:02.75 AS.reconfigure ASnorm @09:32:02.80 AS.seek 0ml @09:32:31.84 ATV.dial :air2 @09:32:32.85 AS.reconfigure ASslow0 @09:32:32.90 AS.seek 0.3ml @09:33:03.48 ATV.dial :ASV @09:33:04.49 ASV.dial :wasteD @09:33:04.51 AS.reconfigure ASconfig @09:33:04.56 AS.seek 0.05ml @09:33:19.24 ATV.dial :waste @09:33:21.14 AS.seek 0ml @09:33:24.30 ASV.dial :SPEdelivery @09:33:25.29 deconLysateINT @09:33:25.31 ATV.dial :air3 @09:33:25.98 AS.reconfigure ASslow1 @09:33:26.03 AS.seek 0.01ml @09:33:28.48 delay 2 seconds @09:33:30.50 ATV.dial :bleach @09:33:30.98 AS.seek 0.96ml @09:36:42.29 delay 3 seconds @09:36:45.32 ATV.dial :air3 @09:36:45.87 delay 3 seconds @09:36:48.96 AS.seek 0.985ml @09:36:54.38 delay 3 seconds @09:36:57.40 ATV.dial :bleach @09:36:58.02 AS.seek 0.97ml @09:37:01.34 delay 3 seconds @09:37:04.37 ATV.dial AIV @09:37:05.65 AIV.dial :coilToMFB @09:37:05.67 AWV.select :waste @09:37:05.68 AFV.select :carrierSolution @09:37:05.79 delay 2 seconds @09:37:07.83 AS.reconfigure ASconfig @09:37:07.87 AS.seek 1ml @09:37:09.91 delay 1 second @09:37:10.93 AFV.select :holdingCoil @09:37:11.03 delay 1 second @09:37:12.07 AS.reconfigure ASslow0 @09:37:12.11 AS.seek :empty @09:38:53.10 AFV.select :carrierSolution @09:38:53.21 delay 2 seconds @09:38:55.25 AS.reconfigure ASconfig @09:38:55.29 AS.seek 1ml @09:39:53.10 delay 1 second @09:39:54.12 AFV.select :holdingCoil @09:39:54.22 delay 1 second @09:39:55.26 AS.reconfigure ASslow0 @09:39:55.30 AS.seek :empty @09:41:36.24 AFV.select :carrierSolution @09:41:36.35 delay 2 seconds @09:41:38.39 AS.reconfigure ASconfig @09:41:38.43 AS.seek 1ml @09:42:36.85 delay 1 second @09:42:37.87 AFV.select :holdingCoil @09:42:37.97 delay 1 second @09:42:39.01 AS.reconfigure ASslow0 @09:42:39.05 AS.seek :empty @09:44:20.04 ATV.dial :air2 @09:44:20.73 AS.reconfigure ASslow1 @09:44:20.79 AS.seek 0.009ml @09:44:23.20 AS.reconfigure ASslow0 @09:44:23.25 AS.seek 1ml @09:46:03.23 ATV.dial AIV @09:46:04.02 AS.seek 0.02ml @09:47:42.91 ATV.dial :waste @09:47:44.58 AS.seek 0ml @09:47:46.90 AWV.select :air @09:47:47.07 Cleaning PCR @09:47:47.08 AFV.select :carrierSolution @09:47:47.18 delay 2 seconds @09:47:49.22 AS.reconfigure ASconfig @09:47:49.26 AS.seek 0.5ml @09:48:18.32 delay 1 second @09:48:19.34 AFV.select :holdingCoil @09:48:19.44 ASV.dial :waste1 @09:48:19.82 ATV.dial :air3 @09:48:20.53 AS.reconfigure ASslow1 @09:48:20.58 AS.seek 0.51ml @09:48:22.85 delay 2 seconds @09:48:24.87 ATV.dial :bleach @09:48:25.34 AS.seek 0.81ml @09:49:25.96 delay 3 seconds @09:49:28.98 ATV.dial :air3 @09:49:29.54 delay 3 seconds @09:49:32.63 AS.seek 0.835ml @09:49:37.93 delay 3 seconds @09:49:40.96 ATV.dial :bleach @09:49:41.59 AS.seek 0.82ml @09:49:44.91 delay 3 seconds @09:49:47.93 ARV.dial :waste @09:49:48.55 delay 3 seconds @09:49:51.58 ATV.dial :ARV @09:49:52.48 AS.reconfigure ASconfig @09:49:52.53 AS.seek 0.47ml @09:50:12.95 AFV.select :carrierSolution @09:50:13.06 delay 2 seconds @09:50:15.10 AS.seek 1ml @09:50:45.88 delay 1 second @09:50:46.90 AFV.select :holdingCoil @09:50:47.00 delay 1 second @09:50:48.04 AS.reconfigure ASfast @09:50:48.08 AS.seek 0ml @09:51:15.41 AFV.select :carrierSolution @09:51:15.52 delay 2 seconds @09:51:17.56 AS.reconfigure ASconfig @09:51:17.60 AS.seek 1ml @09:51:19.99 Session Begun @09:51:30.28 -> showlog 10 @09:52:15.38 delay 1 second @09:52:16.41 AFV.select :holdingCoil @09:52:16.52 delay 1 second @09:52:17.56 AS.reconfigure ASfast @09:52:17.64 AS.seek 0ml @09:52:45.01 AFV.select :carrierSolution @09:52:45.14 delay 2 seconds @09:52:47.18 AS.reconfigure ASconfig @09:52:47.25 AS.seek 1ml @09:53:32.63 -> exit @09:53:32.65 Session Ended @09:53:36.86 Session Begun @09:53:45.05 delay 1 second @09:53:46.07 AFV.select :holdingCoil @09:53:46.21 delay 1 second @09:53:47.25 AS.reconfigure ASfast @09:53:47.32 AS.seek 0ml @09:54:14.71 ARV.dial between carrier and air1 @09:54:15.24 ATV.dial :air3 @09:54:16.58 AS.reconfigure ASslow1 @09:54:16.66 AS.seek 0.01ml @09:54:19.09 delay 2 seconds @09:54:21.11 ATV.dial :bleach @09:54:21.77 AS.seek 0.81ml @09:57:03.02 delay 3 seconds @09:57:06.05 ATV.dial :air3 @09:57:06.62 delay 3 seconds @09:57:09.71 AS.seek 0.835ml @09:57:15.17 delay 3 seconds @09:57:18.20 ATV.dial :bleach @09:57:18.84 AS.seek 0.82ml @09:57:22.27 delay 3 seconds @09:57:25.29 ATV.dial PCR @09:57:25.72 AS.reconfigure ASconfig @09:57:25.79 AS.seek :empty @09:58:13.29 PCR.program :bleachProfile @09:58:13.37 PCR program should complete in < 2:45 @09:58:13.38 PCR.run 10:00 @10:01:21.83 PCR.off @10:01:21.88 AFV.select :carrierSolution @10:01:22.00 delay 2 seconds @10:01:24.04 AS.seek 1ml @10:02:21.88 delay 1 second @10:02:22.90 AFV.select :holdingCoil @10:02:23.03 delay 1 second @10:02:24.07 AS.reconfigure ASfast @10:02:24.14 AS.seek 0ml @10:02:51.49 delay 3 seconds @10:02:54.52 AFV.select :carrierSolution @10:02:54.65 delay 2 seconds @10:02:56.69 AS.reconfigure ASconfig @10:02:56.76 AS.seek 1ml @10:03:54.57 delay 1 second @10:03:55.59 AFV.select :holdingCoil @10:03:55.72 delay 1 second @10:03:56.76 AS.reconfigure ASfast @10:03:56.83 AS.seek 0ml @10:04:24.18 delay 3 seconds @10:04:27.21 AFV.select :carrierSolution @10:04:27.34 delay 2 seconds @10:04:29.38 AS.reconfigure ASconfig @10:04:29.46 AS.seek 1ml @10:05:27.34 delay 1 second @10:05:28.36 AFV.select :holdingCoil @10:05:28.49 delay 1 second @10:05:29.53 AS.reconfigure ASfast @10:05:29.60 AS.seek 0ml @10:05:56.98 delay 3 seconds @10:06:00.01 AFV.select :carrierSolution @10:06:00.14 delay 2 seconds @10:06:02.18 AS.reconfigure ASconfig @10:06:02.26 AS.seek 1ml @10:07:00.05 delay 1 second @10:07:01.07 AFV.select :holdingCoil @10:07:01.20 delay 1 second @10:07:02.24 AS.reconfigure ASfast @10:07:02.31 AS.seek 0ml @10:07:29.68 delay 3 seconds @10:07:32.71 AFV.select :carrierSolution @10:07:32.84 delay 2 seconds @10:07:34.88 AS.reconfigure ASconfig @10:07:35.00 AS.seek 1ml @10:08:32.80 delay 1 second @10:08:33.82 AFV.select :holdingCoil @10:08:33.95 delay 1 second @10:08:34.99 AS.reconfigure ASnorm @10:08:35.06 AS.seek 0ml @10:09:25.80 AFV.select :carrierSolution @10:09:25.93 delay 2 seconds @10:09:27.97 AS.reconfigure ASconfig @10:09:28.04 AS.seek 1ml @10:10:25.80 delay 1 second @10:10:26.82 AFV.select :holdingCoil @10:10:26.94 delay 1 second @10:10:27.97 ATV.dial :waste @10:10:29.24 AS.reconfigure ASnorm @10:10:29.32 AS.seek 0.5ml @10:10:54.88 ATV.dial :waste2 @10:10:56.22 AS.seek 0ml @10:11:21.80
Asleep after completing 190523totalPostcanqc assays @10:11:21.81 Mission runtime was 11:21:30.778129 @10:11:21.84 GoodNews.email "{1 => 3, 2 => 19}\nCan@10:09:56, 21.6C, 38% humidity, 14.7psia, 12.036V, 0.537A, 0.537A avg, 6.47W",Subject: "Asleep after completing 190523totalPostcanqc assays" @10:11:32.32 Session Ended @12:48:07.04CEST16-Jul-19
ESP2 v3.30 I2C Gateway v3.06 for core bus @12:48:09.18 Copyright 2018 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @12:48:09.47 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @12:48:14.48 Polling Can every 10:00 @12:48:14.50 Cleanly exited at 12:47:33CEST16-Jul-19 @12:48:14.54 Loading /home/esp/esp2/mission/TotalTRIAL.rb mission @12:48:14.61 Preparing to sleep until +00:01:00 @12:48:14.64 GoodNews.email "Starting TotalTRIAL w/pucks in tube 2\n{}\nCan@12:48:14, 23.1C, 71% humidity, 14.9psia, 12.124V, 0.172A, 0.172A avg, 2.08W",Subject: "TotalTRIAL begun" @12:48:14.73 Shutdown @12:48:14.75
Asleep til +00:01:00 @12:48:14.77 Continuing at 12:49 @12:48:17.72 Session Begun @12:48:21.79 GoodNews.email "Then, in tube 2: wcrPhase2\n/home/esp/esp2/mission/TotalTRIAL.rb:113\n{}\nCan@12:48:14, 23.1C, 71% humidity, 14.9psia, 12.124V, 0.172A, 0.172A avg, 2.08W",Subject: "Asleep until 12:49" @12:49:14.58
Powering core bus @12:49:19.27 Gate.power :raw,:ON @12:49:19.95 SPE.coast @12:49:20.04 SPE.configure SPEconfig @12:49:20.17 AS.configure ASconfig @12:49:20.29 SS.configure SSconfig @12:49:20.41 CC.configure CCconfig @12:49:20.52 CS.configure CSconfig @12:49:20.64 PC.configure PCconfig @12:49:20.77 PS.configure PSconfig @12:49:20.91 Forearm.configure LooseForearm @12:49:21.03 Elbow.configure LooseElbow @12:49:21.17 SC.configure SCloose @12:49:21.29 SE.configure SEconfig @12:49:21.36 CH.coast @12:49:21.44 CH.configure CHconfig @12:49:21.49 PH.coast @12:49:21.57 PH.configure PHconfig @12:49:21.63 PCR.off @12:49:21.67 PCR.pidtemp= 50,0.1,0,300,25,25 @12:49:21.71 PCR.caltemp= 2.8814,0.0372,0 @12:49:21.76 All dwarf controllers running firmware version 3.75 @12:49:21.90 Valve.reset @12:49:22.09 PCR.off @12:49:22.13 PCR.pidtemp= 50,0.1,0,300,25,25 @12:49:22.17 PCR.caltemp= 2.8814,0.0372,0 @12:49:22.31 Intake.select :close @12:49:22.40 Exhaust.select :close @12:49:22.41 CTV.dial :air @12:49:22.52 PSV.dial :air @12:49:22.56 CSV.dial :waste1 @12:49:22.76 SSV.dial :spit,avoiding: :exhaust @12:49:22.81 PS.reconfigure PSconfig @12:49:22.84
Forearm.reconfigure LooseForearm @12:49:23.00 PS.home.seek 0ml @12:49:23.05
Forearm.home.seek :retract @12:49:23.30 CS.reconfigure CSfast @12:49:23.34 SS.reconfigure SSconfig @12:49:23.43 CS.home.seek 0ml @12:49:23.52 SS.home.seek 0ml @12:49:23.92 Hand.close @12:49:23.96
Elbow.jog 1500 @12:49:24.47 Elbow.reconfigure LooseElbow @12:49:24.54 Elbow.home.seek :clear @12:49:29.23 SE.reconfigure SEconfig @12:49:29.31 SE.home.seek :down @12:49:30.18 SC.reconfigure SCsloppy @12:49:30.25 SC.home.seek :initial @12:49:32.32 PS.coast @12:49:34.25
Forearm.seek :retract @12:49:34.36 Elbow.seek :hammer @12:49:35.76 Forearm.reconfigure TightForearm @12:49:35.84 Forearm.seek 5 + 350 counts @12:49:36.93 Forearm.seek :retract @12:49:38.15 PC.reconfigure PCconfig @12:49:38.17
CC.reconfigure CCconfig @12:49:38.30 CC.home.seek :open @12:49:38.32 PC.home.seek :open @12:49:41.68
Phase [wcrPhase2] @12:49:41.70 GoodNews.email "At 12:49:41 began in tube 2: wcrPhase2\n/home/esp/esp2/mission/TotalTRIAL.rb:113\n{}\nCan@12:49:41, 23.0C, 70% humidity, 14.9psia, 11.962V, 0.809A, 0.809A avg, 9.67W",Subject: "Started TotalTRIAL phase wcrPhase2" @12:49:42.40
Completed [wcrPhase2] begun 12:49:41 @12:49:42.46 Asleep after completing TotalTRIAL assays @12:49:42.48 Mission runtime was 1:27.901062 @12:49:46.82 GoodNews.email "{}\nCan@12:49:41, 23.0C, 70% humidity, 14.9psia, 11.962V, 0.809A, 0.809A avg, 9.67W",Subject: "Asleep after completing TotalTRIAL assays" @12:49:51.95 Session Ended @14:57:58.69CEST05-Sep-19
ESP2 v3.30 I2C Gateway v3.06 for core bus @14:58:00.37 Copyright 2018 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @14:58:00.64 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @14:58:04.97 Polling Can every 10:00 @14:58:06.72 SPE.coast @14:58:06.76 SPE.configure SPEconfig @14:58:06.85 AS.configure ASconfig @14:58:06.93 SS.reconfigure SSconfig @14:58:06.97 SS.hold @14:58:07.02 SS.configure SSconfig @14:58:07.11 CC.reconfigure CCconfig @14:58:07.14 CC.hold @14:58:07.21 CC.configure CCconfig @14:58:07.29 CS.reconfigure CSconfig @14:58:07.33 CS.hold @14:58:07.39 CS.configure CSconfig @14:58:07.47 PC.reconfigure PCconfig @14:58:07.51 PC.hold @14:58:07.90 PC.configure PCconfig @14:58:07.98 PS.configure PSconfig @14:58:08.08 Forearm.reconfigure LooseForearm @14:58:08.12 Forearm.hold @14:58:08.18 Forearm.configure LooseForearm @14:58:08.26 Elbow.reconfigure LooseElbow @14:58:08.30 Elbow.hold @14:58:08.35 Elbow.configure LooseElbow @14:58:08.45 SC.reconfigure SCloose @14:58:08.49 SC.hold @14:58:08.55 SC.configure SCloose @14:58:08.63 SE.reconfigure SEconfig @14:58:08.67 SE.hold @14:58:08.72 SE.configure SEconfig @14:58:08.76 CH.coast @14:58:08.80 CH.configure CHconfig @14:58:08.83 PH.coast @14:58:08.88 PH.configure PHconfig @14:58:08.91 PCR.off @14:58:08.94 PCR.pidtemp= 50,0.1,0,300,25,25 @14:58:08.97 PCR.caltemp= 2.8814,0.0372,0 @14:58:09.00 All dwarf controllers running firmware version 3.75 @14:58:09.12 Cleanly exited at 14:32:55CEST05-Sep-19 @14:58:09.15 Loading /home/esp/esp2/mission/190905totalPostcanqc.rb mission @14:58:09.20 Preparing to sleep until +0:00:15 @14:58:09.22 GoodNews.email "Starting 190905totalPostcanqc w/pucks in tube 2\n{}\nCan@14:58:04, 21.9C, 77% humidity, 14.9psia, 11.977V, 0.676A, 0.676A avg, 8.10W",Subject: "190905totalPostcanqc begun" @14:58:09.30 SS.coast @14:58:09.32
Asleep til +0:00:15 @14:58:09.35 Continuing at 14:58 @14:58:09.38 CC.coast @14:58:09.45 CS.coast @14:58:09.48 PC.coast @14:58:09.51 Forearm.coast @14:58:09.54 Elbow.coast @14:58:09.57 SC.coast @14:58:09.61 SE.coast @14:58:09.99 Gate.power :core,:OFF @14:58:10.01 Gate.power :raw,:OFF @14:58:10.02 Shutdown @14:58:16.82 Session Begun @14:58:17.42 GoodNews.email "Then, in tube 2: mbaPhase\n/home/esp/esp2/mission/190905totalPostcanqc.rb:111\n{}\nCan@14:58:09, 22.0C, 76% humidity, 14.9psia, 11.982V, 0.676A, 0.676A avg, 8.10W",Subject: "Asleep until 14:58" @14:58:24.23
Powering core bus @14:58:29.38 Gate.power :raw,:ON @14:58:31.26 SPE.coast @14:58:31.43 SPE.configure SPEconfig @14:58:31.73 AS.configure ASconfig @14:58:32.03 SS.configure SSconfig @14:58:32.33 CC.configure CCconfig @14:58:32.67 CS.configure CSconfig @14:58:33.03 PC.configure PCconfig @14:58:33.35 PS.configure PSconfig @14:58:33.73 Forearm.configure LooseForearm @14:58:34.03 Elbow.configure LooseElbow @14:58:34.40 SC.configure SCloose @14:58:34.70 SE.configure SEconfig @14:58:34.88 CH.coast @14:58:35.14 CH.configure CHconfig @14:58:35.24 PH.coast @14:58:35.45 PH.configure PHconfig @14:58:35.62 PCR.off @14:58:35.71 PCR.pidtemp= 50,0.1,0,300,25,25 @14:58:35.83 PCR.caltemp= 2.8814,0.0372,0 @14:58:35.89 All dwarf controllers running firmware version 3.75 @14:58:36.14 Valve.reset @14:58:36.49 PCR.off @14:58:36.59 PCR.pidtemp= 50,0.1,0,300,25,25 @14:58:36.70 PCR.caltemp= 2.8814,0.0372,0 @14:58:36.98 Intake.select :close @14:58:37.10 Exhaust.select :close @14:58:37.28 CTV.dial :air @14:58:37.79 PSV.dial :air @14:58:37.92 CSV.dial :waste1 @14:58:38.40 SSV.dial :spit,avoiding: :exhaust @14:58:38.48
Forearm.reconfigure LooseForearm @14:58:38.57 PS.reconfigure PSconfig @14:58:38.94 PS.home.seek 0ml @14:58:39.02
Forearm.home.seek :retract @14:58:40.65 CS.reconfigure CSfast @14:58:40.74 SS.reconfigure SSconfig @14:58:41.01 CS.home.seek 0ml @14:58:41.12 SS.home.seek 0ml @14:58:41.41 Hand.close @14:58:41.52
Elbow.jog 1500 @14:58:42.36 Elbow.reconfigure LooseElbow @14:58:42.58 Elbow.home.seek :clear @14:58:46.26 SE.reconfigure SEconfig @14:58:46.42 SE.home.seek :down @14:58:47.35 SC.reconfigure SCsloppy @14:58:47.52 SC.home.seek :initial @14:58:48.31 PS.coast @14:58:51.75
Forearm.seek :retract @14:58:52.14 Elbow.seek :hammer @14:58:53.63 Forearm.reconfigure TightForearm @14:58:53.91 Forearm.seek 5 + 350 counts @14:58:55.30 Forearm.seek :retract @14:58:57.17 PC.reconfigure PCconfig @14:58:57.28
CC.reconfigure CCconfig @14:58:57.61 PC.home.seek :open @14:58:57.73
CC.home.seek :open @14:59:02.00 Phase [mbaPhase] @14:59:02.05 GoodNews.email "At 14:59:01 began in tube 2: mbaPhase\n/home/esp/esp2/mission/190905totalPostcanqc.rb:111\n{}\nCan@14:59:01, 21.9C, 76% humidity, 14.9psia, 12.041V, 0.449A, 0.449A avg, 5.41W",Subject: "Started 190905totalPostcanqc phase mbaPhase" @14:59:02.15CEST05-Sep-19
==================> STARTED MBAsuperphase (phase #1) <=== @14:59:04.45 Readying the MFB @14:59:04.50 Homing MFB Syringe @14:59:04.57 AFV.select :holdingCoil @14:59:05.03 ATV.dial :air2 @14:59:06.61 AS.reconfigure ASconfig @14:59:06.79 AS.home.seek :home @14:59:12.50 AFV.select :carrierSolution @14:59:12.74 delay 2 seconds @14:59:14.82 AS.seek 1ml @15:00:07.89 delay 1 second @15:00:08.92 AFV.select :holdingCoil @15:00:09.12 delay 1 second @15:00:10.18 ATV.dial :waste @15:00:11.59 AS.reconfigure ASnorm @15:00:11.85 AS.home.seek :empty @15:01:04.52 ATV.dial :waste @15:01:04.54 ARV.dial between carrier and air1 @15:01:04.65 ASV.dial :waste1 @15:01:04.82 Starting mfb @15:01:04.83 pcrBAC sampling at most 100ml, wcr at most 100ml @15:01:04.86 BACfans.email "Started mfb in tube 2 at 15:01:04CEST05-Sep-19\n{}\nCan@14:59:01, 21.9C, 76% humidity, 14.9psia, 12.041V, 0.449A, 0.449A avg, 5.41W",Subject: "pcrBAC sampling at most 100ml, wcr at most 100ml" @15:01:04.92
Loading sh1 puck from tube 2 into Collection Clamp @15:01:05.07 Hand.open @15:01:06.09 SC.reconfigure SCloose @15:01:06.28 Elbow.reconfigure TightElbow @15:01:06.34 Elbow.seek 2 @15:01:06.81 Forearm.seek 2 + 700 counts @15:01:07.97 SE.reconfigure SEpick @15:01:08.07 SE.seek beamBreak @15:01:09.47 SE.configure SEconfig @15:01:09.72 Elbow.seek 2 @15:01:10.11 Forearm.seek 2 + 300 counts @15:01:10.43 Forearm.reconfigure LooseForearm @15:01:10.50 Hand.close @15:01:11.28 SE.seek :down @15:01:11.50
Forearm.seek :retract @15:01:12.44 SE.coast @15:01:12.60
Elbow.seek CC @15:01:15.04 Elbow.reconfigure LooseElbow @15:01:15.26 Forearm.reconfigure TightForearm @15:01:15.37 Forearm.seek CC @15:01:16.95 CC.seek :closed @15:01:21.39 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.21 @15:01:21.41 Hand.open @15:01:22.38 Forearm.reconfigure LooseForearm @15:01:22.49 Forearm.seek :retract @15:01:23.60 SC.coast @15:01:23.70 collectSample 100ml w/bubble point of 55psi @15:01:23.73 Priming 7ml @15:01:23.76 Gate.power :sampler,:ON @15:01:23.81 Intake.select :close @15:01:23.85 Exhaust.select :close @15:01:23.89 SS.hold @15:01:24.34 Calibrating pressure and temperature sensors @15:01:24.39 CTV.dial :air @15:01:24.43 SSV.dial :spit,avoiding: :exhaust @15:01:24.50 SS.reconfigure SSfast @15:01:24.62 SS.seek 5ml @15:01:27.71 SSV.dial CBV @15:01:27.74
CBV.dial SSV @15:01:29.28 delay 3.8 seconds @15:01:33.19 SSV.dial :spit @15:01:34.70 SS.seek 0ml @15:01:37.30 SSV.dial CBV @15:01:38.81 CTV.dial :intake @15:01:39.68 Waiting for external sampler to finish priming... @15:01:39.70 delayUntil "15:06:23.81" @15:06:23.83 SSV.dial CBV @15:06:23.85
CBV.dial SSV @15:06:23.89 Allowing 40s for 7.0ml of remaining 7.0ml @15:06:23.91 Intake.select :open @15:06:26.09 Intake @15.5psia, vacuum from 9.0 to 13.0psi @15:06:26.11 SS.reconfigure SSinhale.with maxSpeed: 150 @15:06:26.19 SS.seek 7ml @15:06:32.60 Stroke completed -- waiting for pressure to equalize ... @15:06:46.56 Intake.select :close @15:06:46.71 SSV.dial :exhaust @15:06:47.57 Exhaust.select :open @15:06:47.70 SS.reconfigure SSexhale @15:06:47.77 SS.seek 0ml @15:06:52.07 Exhaust.select :close @15:06:52.21 Sampling 100.0ml @15:06:52.23 Intake.select :close @15:06:52.24 Exhaust.select :close @15:06:52.27 SS.reconfigure SSconfig @15:06:52.34 SS.hold @15:06:52.54 CTV.dial :intake @15:06:52.98 SSV.dial CBV @15:06:53.02
CBV.dial SSV @15:06:53.91 Allowing 130s for 25.0ml of remaining 100.0ml @15:06:53.93 Intake.select :open @15:06:56.12 Intake @15.7psia, vacuum from 9.2 to 13.2psi @15:06:56.14 SS.reconfigure SSinhale.with maxSpeed: 150 @15:06:56.22 SS.seek 25ml @15:06:59.90 Vacuum too high -- waiting up to 40s @15:07:04.56 Intake @15.4psia, vacuum from 8.9 to 12.9psi @15:07:04.58 SS.reconfigure SSinhale.with maxSpeed: 135 @15:07:04.66 SS.seek 25ml @15:07:07.96 Vacuum too high -- waiting up to 40s @15:07:12.85 Intake @15.5psia, vacuum from 9.0 to 13.0psi @15:07:12.86 SS.reconfigure SSinhale.with maxSpeed: 122 @15:07:12.94 SS.seek 25ml @15:07:16.09 Vacuum too high -- waiting up to 40s @15:07:20.27 SS.reconfigure SSinhale.with maxSpeed: 110 @15:07:20.34 SS.seek 25ml @15:07:23.44 Vacuum too high -- waiting up to 40s @15:07:27.19 SS.reconfigure SSinhale.with maxSpeed: 99 @15:07:27.26 SS.seek 25ml @15:07:30.52 Vacuum too high -- waiting up to 40s @15:07:34.50 Intake @15.6psia, vacuum from 9.1 to 13.1psi @15:07:34.52 SS.reconfigure SSinhale.with maxSpeed: 89 @15:07:34.60 SS.seek 25ml @15:07:38.15 Vacuum too high -- waiting up to 40s @15:07:41.67 SS.reconfigure SSinhale.with maxSpeed: 80 @15:07:41.74 SS.seek 25ml @15:07:45.60 Vacuum too high -- waiting up to 40s @15:07:49.12 Intake @15.5psia, vacuum from 9.0 to 13.0psi @15:07:49.14 SS.reconfigure SSinhale.with maxSpeed: 72 @15:07:49.21 SS.seek 25ml @15:07:49.69 Stroke completed -- waiting for pressure to equalize ... @15:07:51.57 Intake.select :close @15:07:51.72 SSV.dial :exhaust @15:07:52.60 Exhaust.select :open @15:07:52.73 SS.reconfigure SSexhale @15:07:52.80 SS.seek 0ml @15:08:02.27 Exhaust.select :close @15:08:02.40 SSV.dial CBV @15:08:02.42
CBV.dial SSV @15:08:03.32 Allowing 130s for 25.0ml of remaining 75.0ml @15:08:03.34 Intake.select :open @15:08:05.52 Intake @15.8psia, vacuum from 9.3 to 13.3psi @15:08:05.54 SS.reconfigure SSinhale.with maxSpeed: 72 @15:08:05.61 SS.seek 25ml @15:08:10.09 Vacuum too high -- waiting up to 40s @15:08:13.14 Intake @15.5psia, vacuum from 9.0 to 13.0psi @15:08:13.16 SS.reconfigure SSinhale.with maxSpeed: 65 @15:08:13.23 SS.seek 25ml @15:08:17.90 Vacuum too high -- waiting up to 40s @15:08:20.94 Intake @15.5psia, vacuum from 9.0 to 13.0psi @15:08:20.96 SS.reconfigure SSinhale.with maxSpeed: 59 @15:08:21.03 SS.seek 25ml @15:08:27.12 Vacuum too high -- waiting up to 40s @15:08:30.62 Intake @15.5psia, vacuum from 9.0 to 13.0psi @15:08:30.63 SS.reconfigure SSinhale.with maxSpeed: 53 @15:08:30.71 SS.seek 25ml @15:08:39.12 Vacuum too high -- waiting up to 40s @15:08:42.64 Intake @15.6psia, vacuum from 9.1 to 13.1psi @15:08:42.66 SS.reconfigure SSinhale.with maxSpeed: 48 @15:08:42.73 SS.seek 25ml @15:08:53.38 Stroke completed -- waiting for pressure to equalize ... @15:08:58.88 Intake.select :close @15:08:59.04 SSV.dial :exhaust @15:08:59.91 Exhaust.select :open @15:09:00.04 SS.reconfigure SSexhale @15:09:00.11 SS.seek 0ml @15:09:09.81 Exhaust.select :close @15:09:09.93 SSV.dial CBV @15:09:09.95
CBV.dial SSV @15:09:10.85 Allowing 130s for 25.0ml of remaining 50.0ml @15:09:10.87 Intake.select :open @15:09:13.05 Intake @15.7psia, vacuum from 9.2 to 13.2psi @15:09:13.07 SS.reconfigure SSinhale.with maxSpeed: 48 @15:09:13.15 SS.seek 25ml @15:09:20.27 Vacuum too high -- waiting up to 40s @15:09:22.39 Intake @15.4psia, vacuum from 8.9 to 12.9psi @15:09:22.41 SS.reconfigure SSinhale.with maxSpeed: 43 @15:09:22.49 SS.seek 25ml @15:09:36.68 Vacuum too high -- waiting up to 40s @15:09:39.48 Intake @15.5psia, vacuum from 9.0 to 13.0psi @15:09:39.49 SS.reconfigure SSinhale.with maxSpeed: 39 @15:09:39.57 SS.seek 25ml @15:10:01.58 Stroke completed -- waiting for pressure to equalize ... @15:10:05.89 Intake.select :close @15:10:06.05 SSV.dial :exhaust @15:10:06.94 Exhaust.select :open @15:10:07.07 SS.reconfigure SSexhale @15:10:07.14 SS.seek 0ml @15:10:16.28 Exhaust.select :close @15:10:16.40 SSV.dial CBV @15:10:16.42
CBV.dial SSV @15:10:17.31 Allowing 130s for 25.0ml of remaining 25.0ml @15:10:17.33 Intake.select :open @15:10:19.51 Intake @15.8psia, vacuum from 9.3 to 13.3psi @15:10:19.53 SS.reconfigure SSinhale.with maxSpeed: 39 @15:10:19.61 SS.seek 25ml @15:11:05.47 Stroke completed -- waiting for pressure to equalize ... @15:11:10.97 Intake.select :close @15:11:11.12 SSV.dial :exhaust @15:11:11.99 Exhaust.select :open @15:11:12.12 SS.reconfigure SSexhale @15:11:12.19 SS.seek 0ml @15:11:21.63 Exhaust.select :close @15:11:21.76 Sampled 100.0ml @15:11:21.77 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @15:11:21.79 Intake.select :close @15:11:21.88 CSV.dial :waste @15:11:21.96 CS.seek 0ml @15:11:22.05 SSV.dial CBV,avoiding: :exhaust @15:11:22.08 CSV.dial :air @15:11:22.12
CBV.dial SSV @15:11:23.00 CS.seek 10ml @15:11:24.29
CTV.dial :air @15:11:24.76 Gate.power :sampler,:OFF @15:11:25.18
delay 15 seconds @15:11:40.20 SSV.dial :spit,avoiding: :exhaust @15:11:41.71 CTV.dial CSV,avoiding: :intake @15:11:41.75
SS.seek 0ml @15:11:41.90 SSV.dial CBV,avoiding: :exhaust @15:11:50.44 CBV.dial between SSV and waste2 @15:11:50.87 CTV.dial CSV,avoiding: :air @15:11:50.90
CSV.dial CTV @15:11:51.44 CS.reconfigure CSpressurize @15:11:51.51 CS.seek 0ml @15:12:18.01 CSV.dial :air @15:12:18.55 CS.reconfigure CSfast @15:12:18.61 CS.seek 10ml @15:12:45.51 CSV.dial between air and CTV @15:12:45.88 CS.seek 2.458ml @15:13:06.85 CTV.dial CSV,avoiding: :air @15:13:06.87
CSV.dial CTV @15:13:07.24 CS.reconfigure CSpressurize @15:13:07.31 CS.seek 0ml @15:13:10.64 BubblePoint: Intake @70.5psia, Outlet @15.0psia @15:13:10.65 CBV.dial SSV @15:13:11.04 SS.reconfigure SSconfig @15:13:11.10 SS.seek 10ml @15:13:16.99 delay 15 seconds @15:13:32.12 Evacuated: Intake @34.2psia, Outlet @14.4psia @15:13:32.13 SSV.dial :spit,avoiding: :exhaust @15:13:33.67 SS.reconfigure SSexhale @15:13:33.74 SS.seek 0ml @15:13:38.85 SS.reconfigure SSfast @15:13:38.92 SS.seek 5ml @15:13:41.96 SSV.dial CBV,avoiding: :exhaust @15:13:41.99
CBV.dial SSV @15:13:43.52 CTV.dial :air,avoiding: :intake @15:13:45.07 CSV.dial CTV @15:13:45.12
SS.seek 10ml @15:13:46.09 CSV.dial :air @15:13:46.63 CS.reconfigure CSfast @15:13:46.69 CS.seek 0ml @15:13:48.02
SSV.dial :spit,avoiding: :exhaust @15:13:49.56 SS.reconfigure SSexhale @15:13:49.63 SS.seek 0ml @15:13:51.39 CS.coast @15:13:54.71
SS.reconfigure SSfast @15:13:54.79 SS.seek 5ml @15:13:57.84 SSV.dial CBV,avoiding: :exhaust @15:13:57.87
CBV.dial SSV @15:14:00.41 SSV.dial :spit,avoiding: :exhaust @15:14:01.93 SS.reconfigure SSconfig @15:14:01.99 SS.seek 0ml @15:14:05.06 SS.coast @15:14:05.12 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) Intake.select :close @15:14:05.22 CSV.dial :waste @15:14:05.35 SSV.dial CBV,avoiding: :exhaust @15:14:05.37
CBV.dial SSV @15:14:06.13 CS.seek 0ml @15:14:06.23 CSV.dial :air @15:14:06.93
CTV.dial :air @15:14:06.94 delay 15 seconds @15:14:07.10 CS.seek 10ml @15:14:21.97
SSV.dial :spit,avoiding: :exhaust @15:14:23.49 CTV.dial CSV,avoiding: :intake @15:14:23.53
SS.reconfigure SSexhale @15:14:23.64 SS.seek 0ml @15:14:23.88 SSV.dial CBV,avoiding: :exhaust @15:14:34.29 CBV.dial between SSV and waste2 @15:14:34.74 CSV.dial between air and CTV @15:14:35.10 CS.seek 8.252ml @15:14:40.68 CTV.dial CSV,avoiding: :air @15:14:40.70
CSV.dial CTV @15:14:41.07 CS.reconfigure CSpressurize @15:14:41.13 CS.seek 0ml @15:15:02.95 CSV.dial :air @15:15:03.49 CS.reconfigure CSfast @15:15:03.55 CS.seek 10ml @15:15:30.17 CSV.dial between air and CTV @15:15:30.66 CS.seek 3.152ml @15:15:49.46 CTV.dial CSV,avoiding: :air @15:15:49.49
CSV.dial CTV @15:15:49.85 CS.reconfigure CSpressurize @15:15:49.92 CS.seek 0ml @15:15:58.27 BubblePoint: Intake @69.9psia, Outlet @14.6psia @15:15:58.28 CBV.dial SSV @15:15:58.68 SS.reconfigure SSconfig @15:15:58.74 SS.seek 10ml @15:16:04.08 delay 15 seconds @15:16:19.63 Evacuated: Intake @37.0psia, Outlet @17.8psia @15:16:19.64 SSV.dial :spit,avoiding: :exhaust @15:16:21.17 SS.reconfigure SSexhale @15:16:21.24 SS.seek 0ml @15:16:26.16 SS.reconfigure SSfast @15:16:26.23 SS.seek 5ml @15:16:29.07 SSV.dial CBV,avoiding: :exhaust @15:16:29.09
CBV.dial SSV @15:16:30.62 CTV.dial :air,avoiding: :intake @15:16:32.18 CSV.dial CTV @15:16:32.21
SS.seek 10ml @15:16:33.22 CSV.dial :air @15:16:33.74 CS.reconfigure CSfast @15:16:33.80 CS.seek 0ml @15:16:34.98
SSV.dial :spit,avoiding: :exhaust @15:16:35.29 CS.coast @15:16:36.53
SS.reconfigure SSexhale @15:16:36.59 SS.seek 0ml @15:16:41.97 SS.reconfigure SSfast @15:16:42.04 SS.seek 5ml @15:16:44.71 SSV.dial CBV,avoiding: :exhaust @15:16:44.74
CBV.dial SSV @15:16:47.27 SSV.dial :spit,avoiding: :exhaust @15:16:48.79 SS.reconfigure SSconfig @15:16:48.85 SS.seek 0ml @15:16:52.05 SS.coast @15:16:52.11 Lysing: Adding 1.5ml lysis @15:16:52.12 CBV.dial between CSV and waste1,avoiding: :DBV @15:16:53.88 CTV.dial CSV @15:16:55.43 CSV.dial CSR @15:16:56.34 Pulling 1ml of CSR.lysis @15:16:56.35 CSR.select :lysis @15:16:56.48 CS.reconfigure CSconfig @15:16:56.55 CS.seek 1ml @15:17:00.07 delay 5 seconds @15:17:05.10 CSR.select :air @15:17:05.27 CS.seek 6ml @15:17:19.01 delay 2 seconds @15:17:21.04 CSV.dial :waste1 @15:17:22.66 CS.reconfigure CSfast @15:17:22.72 CS.seek :empty @15:17:39.17 CSV.dial CSR @15:17:40.85 Pulling 1.5ml of CSR.lysis @15:17:40.86 CSR.select :lysis @15:17:40.99 CS.reconfigure CSconfig @15:17:41.06 CS.seek 1.5ml @15:17:45.98 delay 5 seconds @15:17:51.01 CSR.select :air @15:17:51.19 CS.seek 4.5ml @15:17:59.83 delay 2 seconds @15:18:01.86 CSV.dial CTV @15:18:02.40 CS.seek 1.275ml @15:18:11.68 Heating sh1 puck from tube 2 in Collection Clamp to 85C for 5:00 @15:18:11.70 CH.seek 85C,8:00 @15:21:44.02 CH.hold 5:00,85C @15:26:44.19 Collection Puck Heater at 85C after lysing @15:26:44.21 CS.seek 0ml @15:26:48.96 CTV.dial between spare and air,avoiding: :intake @15:26:50.17 CSV.dial :air @15:26:50.68 CS.seek 5ml @15:27:04.67 CSV.dial CTV @15:27:05.32 delay 2 seconds @15:27:07.34 CSV.dial :air @15:27:07.86 delay 2 seconds @15:27:09.88 CSV.dial CTV @15:27:10.40 delay 2 seconds @15:27:12.42 CSV.dial :air @15:27:12.94 delay 2 seconds @15:27:14.97 CS.seek 0ml @15:27:28.80 Recovering Lysate into Collection syringe @15:27:28.81 CSV.dial CBV @15:27:30.02 CBV.dial CSV @15:27:30.46 CS.seek 5ml @15:27:44.24 delay 5 seconds @15:27:49.27 CTV.dial :air @15:27:49.85 CS.seek 7ml @15:27:56.05 delay 5 seconds @15:28:01.08 CSV.dial :air2 @15:28:01.59 CS.seek 10ml @15:28:10.52 CSV.dial between air and CTV,avoiding: CBV @15:28:11.87 Lysing Complete @15:28:11.95 Storing sh1 puck in Collection Clamp to tube 1 @15:28:12.13 SC.reconfigure SCtight @15:28:12.19 SC.seek 1 @15:28:14.36 SC.reconfigure SCloose @15:28:14.58 Elbow.reconfigure TightElbow @15:28:14.64 Elbow.seek 1 @15:28:16.48 Forearm.reconfigure TightForearm @15:28:16.55 Forearm.seek 1 + 700 counts @15:28:17.59 SE.reconfigure SEpick @15:28:17.68 SE.seek beamBreak @15:28:20.69 SE.configure SEconfig @15:28:20.86 Elbow.reconfigure LimpElbow @15:28:20.92 Forearm.reconfigure LooseForearm @15:28:20.98 Forearm.seek 1 @15:28:21.70 Elbow.hold @15:28:21.85 Elbow.reconfigure TightElbow @15:28:21.96 SE.seek 2.06 Pucks below Top Plate @15:28:22.43 Forearm.force -20 @15:28:22.49 Forearm.seek 1 + 342 counts @15:28:22.81 Forearm.reconfigure TightForearm @15:28:22.88 Forearm.seek 1 + 700 counts @15:28:23.20 SE.seek 3.06 Pucks below Top Plate @15:28:23.81 Forearm.reconfigure LooseForearm @15:28:23.88 Forearm.seek :retract @15:28:24.81 Elbow.seek CC @15:28:26.50 Elbow.reconfigure LooseElbow @15:28:26.61 Forearm.seek CC @15:28:27.69 Hand.close @15:28:28.40 CC.seek :open @15:28:32.89 Forearm.seek :retract @15:28:34.04 Elbow.reconfigure TightElbow @15:28:34.10 Elbow.seek 1 @15:28:36.06 Elbow.reconfigure LimpElbow @15:28:36.12 Forearm.seek 1 @15:28:37.18 Elbow.hold @15:28:37.27 Elbow.reconfigure TightElbow @15:28:37.33 Hand.open @15:28:38.26 Forearm.force -20 @15:28:38.31 Forearm.seek 1 + 345 counts @15:28:38.62 Forearm.reconfigure TightForearm @15:28:38.69 Forearm.seek 1 + 700 counts @15:28:38.96 SE.seek :down @15:28:40.09
Forearm.reconfigure LooseForearm @15:28:40.15 Forearm.seek :retract @15:28:41.52 SE.coast @15:28:41.57
SC.coast @15:28:41.62 Tranferring lysate to PRV @15:28:41.63 CSV.dial :air @15:28:42.00 CS.seek 10ml @15:28:42.08 CSV.dial between air and CTV @15:28:42.45 AIV.dial :bypass @15:28:42.54 Beginning samplePassC @15:28:42.55 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:28:42.65 Hand.open @15:28:43.01 Elbow.seek FlushPuck::Garage @15:28:45.02 Elbow.reconfigure LooseElbow @15:28:45.13 Forearm.seek :garage @15:28:46.18 Hand.close @15:28:47.01 Forearm.seek :retract @15:28:48.50 Elbow.reconfigure TightElbow @15:28:48.55 Elbow.seek CC @15:28:51.45 Elbow.reconfigure LooseElbow @15:28:51.56 Forearm.reconfigure TightForearm @15:28:51.62 Forearm.seek CC @15:28:52.95 CC.seek :closed @15:28:57.29 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.28 @15:28:57.38 Hand.open @15:28:58.20 Forearm.reconfigure LooseForearm @15:28:58.27 Forearm.seek :retract @15:28:59.31 AWV.select :air @15:28:59.33 AIV.dial :bypass @15:28:59.35 PSV.dial PSR @15:29:00.90 Pulling 1.5ml of PSR.flush @15:29:00.91 PSR.select :flush @15:29:01.06 PS.seek 1.5ml @15:29:17.35 delay 5 seconds @15:29:22.37 PSV.dial :waste1 @15:29:22.89 PS.seek 0ml @15:29:39.22 PSV.dial PSR @15:29:39.86 PSR.select :air @15:29:40.00 PS.seek 2.5ml @15:30:06.60 PRV.dial CBV @15:30:06.63
CBV.dial PRV @15:30:07.55 PSV.dial :mixloop @15:30:08.73 CTV.dial :air,avoiding: :intake @15:30:08.75 PTV.dial :PRVmixing @15:30:08.85 PS.seek 0ml @15:30:35.50 PSV.dial :air @15:30:36.01 PS.seek 2.5ml @15:31:02.47 PSV.dial :mixloop @15:31:02.99 PS.seek 0ml @15:31:29.38 PSV.dial :air @15:31:29.90 PS.seek 2.5ml @15:31:56.25 PSV.dial :mixloop @15:31:56.78 PS.seek 0ml @15:32:22.97 Garaging FlushPuck in Collection Clamp @15:32:23.06 Elbow.reconfigure TightElbow @15:32:23.13 Elbow.seek CC @15:32:23.38 Elbow.reconfigure LooseElbow @15:32:23.49 Forearm.seek CC @15:32:24.54 Hand.close @15:32:25.28 CC.seek :open @15:32:29.67 Forearm.seek :retract @15:32:30.75 Elbow.reconfigure TightElbow @15:32:30.81 Elbow.seek :garage @15:32:33.99 Elbow.reconfigure LooseElbow @15:32:34.10 Forearm.reconfigure TightForearm @15:32:34.16 Forearm.seek :garage @15:32:35.35 Hand.open @15:32:36.21 Forearm.reconfigure LooseForearm @15:32:36.27 Forearm.seek :retract @15:32:37.35 Connecting PS to CS to transfer sample @15:32:37.79 CBV.dial AIV @15:32:37.84 AIV.dial :bypass @15:32:37.87 PSV.dial :mixloop @15:32:37.89 PTV.dial :PRVmixing @15:32:37.91 PRV.dial AIV @15:32:37.94 CTV.dial :air,avoiding: :intake @15:32:37.97 Loading lyfil puck from tube 2 into Collection Clamp @15:32:38.21 SC.reconfigure SCtight @15:32:38.27 SC.seek 2 @15:32:40.47 SC.reconfigure SCloose @15:32:40.69 Elbow.reconfigure TightElbow @15:32:40.75 Elbow.seek 2 @15:32:42.78 Forearm.reconfigure TightForearm @15:32:42.85 Forearm.seek 2 + 700 counts @15:32:44.01 SE.reconfigure SEpick @15:32:44.11 SE.seek beamBreak @15:32:45.64 SE.configure SEconfig @15:32:45.81 Elbow.seek 2 @15:32:46.00 Forearm.seek 2 + 300 counts @15:32:46.30 Forearm.reconfigure LooseForearm @15:32:46.37 Hand.close @15:32:47.17 SE.seek :down @15:32:47.37
Forearm.seek :retract @15:32:48.33 Elbow.seek CC @15:32:48.41 SE.coast @15:32:49.95
Elbow.reconfigure LooseElbow @15:32:50.05 Forearm.reconfigure TightForearm @15:32:50.12 Forearm.seek CC @15:32:51.63 CC.seek :closed @15:32:55.89 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.36 @15:32:55.90 Hand.open @15:32:56.72 Forearm.reconfigure LooseForearm @15:32:56.78 Forearm.seek :retract @15:32:57.79 SC.coast @15:32:57.84 CTV.dial CSV,avoiding: :intake @15:32:59.38 CSV.dial CTV @15:32:59.73 CS.seek 5ml @15:33:13.55 delay 1:20 @15:34:33.58 CS.seek 2.5ml @15:34:41.05 delay 40 seconds @15:35:21.08 CS.seek 0ml @15:35:28.58 delay 1:00 @15:36:28.61 PSV.dial :air @15:36:29.12 PS.seek 2.5ml @15:36:55.60 PSV.dial :mixloop @15:36:56.11 delay 3 seconds @15:36:59.14 PSV.dial :air @15:36:59.64 delay 2 seconds @15:37:01.67 PSV.dial :mixloop @15:37:02.16 delay 3 seconds @15:37:05.19 PSV.dial :waste1 @15:37:06.71 PS.seek :empty @15:37:33.22 CBV.dial :waste1 @15:37:33.77 delay 5 seconds @15:37:38.80 CBV.dial AIV @15:37:39.34 delay 10 seconds @15:37:49.38 CBV.dial :waste1 @15:37:49.91 delay 10 seconds @15:37:59.95 CBV.dial AIV @15:38:00.94 delay 10 seconds @15:38:10.97 CTV.dial CSV,avoiding: :intake @15:38:10.98 CBV.dial between AIV and waste1 @15:38:11.38 CSV.dial :air @15:38:11.92 CS.seek 10ml @15:38:38.65 CSV.dial CTV @15:38:39.30 CS.seek 0ml @15:39:06.52 CBV.dial :waste1 @15:39:06.88 delay 10 seconds @15:39:16.91 CTV.dial :air,avoiding: :intake @15:39:18.50 Storing lyfil puck in Collection Clamp to tube 1 @15:39:18.67 SC.reconfigure SCtight @15:39:18.73 SC.seek 1 @15:39:20.93 SC.reconfigure SCloose @15:39:21.15 Elbow.reconfigure TightElbow @15:39:21.21 Elbow.seek 1 @15:39:23.07 Forearm.reconfigure TightForearm @15:39:23.14 Forearm.seek 1 + 700 counts @15:39:24.20 SE.reconfigure SEpick @15:39:24.30 SE.seek beamBreak @15:39:27.25 SE.configure SEconfig @15:39:27.41 Elbow.reconfigure LimpElbow @15:39:27.47 Forearm.reconfigure LooseForearm @15:39:27.53 Forearm.seek 1 @15:39:27.84 Elbow.hold @15:39:27.92 Elbow.reconfigure TightElbow @15:39:28.07 SE.seek 3.07 Pucks below Top Plate @15:39:28.54 Forearm.force -20 @15:39:28.59 Forearm.seek 1 + 342 counts @15:39:28.90 Forearm.reconfigure TightForearm @15:39:28.97 Forearm.seek 1 + 700 counts @15:39:29.35 SE.seek 4.07 Pucks below Top Plate @15:39:30.11 Forearm.reconfigure LooseForearm @15:39:30.17 Forearm.seek :retract @15:39:31.12 Elbow.seek CC @15:39:32.75 Elbow.reconfigure LooseElbow @15:39:32.86 Forearm.seek CC @15:39:33.94 Hand.close @15:39:34.67 CC.seek :open @15:39:39.16 Forearm.seek :retract @15:39:40.24 Elbow.reconfigure TightElbow @15:39:40.30 Elbow.seek 1 @15:39:42.26 Elbow.reconfigure LimpElbow @15:39:42.32 Forearm.seek 1 @15:39:43.37 Elbow.hold @15:39:43.45 Elbow.reconfigure TightElbow @15:39:43.51 Hand.open @15:39:44.40 Forearm.force -20 @15:39:44.46 Forearm.seek 1 + 345 counts @15:39:44.76 Forearm.reconfigure TightForearm @15:39:44.83 Forearm.seek 1 + 700 counts @15:39:45.63 SE.seek :down @15:39:46.76
Forearm.reconfigure LooseForearm @15:39:46.82 Forearm.seek :retract @15:39:47.99 SE.coast @15:39:48.04
SC.coast @15:39:48.09 PSV.dial :air @15:39:49.32 PS.seek 0.499ml @15:39:55.32 delay 3 seconds @15:39:58.35 PSV.dial :mixloop @15:39:58.85 PRV.dial :air @15:39:59.37 delay 3 seconds @15:40:02.39 PRV.dial CBV @15:40:02.89 CBV.dial AIV @15:40:03.41 PRV.dial between CBV and air @15:40:03.78 PRV.dial :air @15:40:04.17 PS.reconfigure PSslow @15:40:04.24 PS.seek 0.599ml @15:40:06.72 PRV.dial :waste2 @15:40:07.55 PS.reconfigure PSconfig @15:40:07.60 PS.seek :empty @15:40:14.58 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:40:14.66 Hand.open @15:40:15.03 Elbow.seek FlushPuck::Garage @15:40:17.03 Elbow.reconfigure LooseElbow @15:40:17.14 Forearm.seek :garage @15:40:18.18 Hand.close @15:40:19.03 Forearm.seek :retract @15:40:20.09 Elbow.reconfigure TightElbow @15:40:20.15 Elbow.seek CC @15:40:22.95 Elbow.reconfigure LooseElbow @15:40:23.06 Forearm.reconfigure TightForearm @15:40:23.12 Forearm.seek CC @15:40:24.43 CC.seek :closed @15:40:28.77 Collection Clamp onSpring/offSpring for 6/30 samples, on/off torque 2.34 @15:40:28.86 Hand.open @15:40:29.72 Forearm.reconfigure LooseForearm @15:40:29.79 Forearm.seek :retract @15:40:30.87 Clearing PML for modified lysate @15:40:30.88 CTV.dial :air,avoiding: :intake @15:40:30.91 PRV.dial :waste2 @15:40:30.93 PTV.dial :PRV,avoiding: :puck @15:40:31.78 delay 2 seconds @15:40:33.80 PTV.dial :PRVmixing,avoiding: :puck @15:40:34.64 delay 2 seconds @15:40:36.66 PSV.dial :PML @15:40:36.68 delay 2 seconds @15:40:38.72 PRV.dial :air @15:40:39.54 delay 2 seconds @15:40:41.61 PS.seek 1.499ml @15:40:57.85 delay 5 seconds @15:41:02.88 PS.seek 2.5ml @15:41:13.82 PSV.dial :air @15:41:14.34 PS.seek 0ml @15:41:41.03 mixing lysate with speDil @15:41:41.04 PSV.dial :PML @15:41:41.57 CBV.dial :PRV @15:41:41.59 PRV.dial :air @15:41:41.62 delay 2 seconds @15:41:43.65 PRV.dial :speDil @15:41:44.17 delay 2 seconds @15:41:46.25 PS.reconfigure PSslow1 @15:41:46.31 PS.seek 0.225ml @15:42:10.44 delay 5 seconds @15:42:15.47 PRV.dial CBV @15:42:16.33 delay 2 seconds @15:42:18.40 PS.seek 0.475ml @15:42:44.66 delay 5 seconds @15:42:49.69 PRV.dial :air @15:42:50.22 delay 2 seconds @15:42:52.24 PS.seek 0.965ml @15:43:42.44 delay 10 seconds @15:43:52.48 PRV.dial CBV @15:43:52.99 CTV.dial :air,avoiding: :intake @15:43:53.01 PSV.dial :air @15:43:53.55 PS.reconfigure PSconfig @15:43:53.61 PS.seek 2.5ml @15:44:09.97 delay 3 seconds @15:44:13.00 PSV.dial PTV @15:44:13.96 PTV.dial :PRV,avoiding: :puck @15:44:14.81 PS.seek 0ml @15:44:41.04 PSV.dial :air @15:44:41.87 PS.seek 2.5ml @15:45:08.29 delay 3 seconds @15:45:11.32 PSV.dial PTV @15:45:12.14 PTV.dial :PRV,avoiding: :puck @15:45:12.17 PS.seek 0ml @15:45:38.48 Pushing modified lysate into AIV Injection Loop CBV.dial AIV @15:45:38.50 AIV.dial :bypass @15:45:38.53 PRV.dial CBV @15:45:38.56 PSV.dial :air @15:45:39.42 PS.seek 2.5ml @15:46:05.72 delay 3 seconds @15:46:08.75 PSV.dial :PML @15:46:09.25 PTV.dial :PRVmixing,avoiding: :puck @15:46:10.17 PS.reconfigure PSslow1 @15:46:10.23 PS.seek 1.74ml @15:47:27.66 delay 5 seconds @15:47:32.69 AIV.dial :sample @15:47:33.32 AWV.select :waste @15:47:33.50 PS.reconfigure PSslow2 @15:47:33.56 PS.seek 1.22ml @15:49:21.19 delay 15 seconds @15:49:36.22 AIV.dial :bypass @15:49:36.87 PS.reconfigure PSconfig @15:49:36.93 PS.seek 0ml @15:49:50.16 PSV.dial :air @15:49:50.69 PS.seek 2.5ml @15:50:16.94 PSV.dial :PML @15:50:17.47 PS.seek 0ml @15:50:43.85 AIV.dial :coilToMFB @15:50:43.87 AWV.select :air @15:50:44.05 Extracting Nucleic Acid from AIV @15:50:44.08 SPE.hold 2:46:40,55C @15:50:44.12
Beginning samplePassD @15:50:44.17 ASV.dial :SPEdelivery @15:50:44.29
AWV.select :air @15:50:44.30 AIV.dial :bypass @15:50:44.33 PSV.dial PSR @15:50:44.67 delay 20 seconds @15:50:45.63
Pulling 1.5ml of PSR.killMFB @15:50:45.64 PSR.select :killMFB @15:50:45.79 PS.seek 1.5ml @15:51:02.77 delay 5 seconds @15:51:04.69 primeLysateINT @15:51:04.70 AWV.select :air @15:51:04.71 AIV.dial :coilToMFB @15:51:04.74 ATV.dial AIV @15:51:06.68 AS.reconfigure ASslow1 @15:51:06.75 AS.seek 0.299ml @15:51:07.79
PSV.dial :waste1 @15:51:08.77 PS.seek 0ml @15:51:25.82 PSV.dial PSR @15:51:26.36 PSR.select :air @15:51:26.51 PS.seek 2.5ml @15:51:54.17 PRV.dial CBV @15:51:54.20
CBV.dial PRV @15:51:54.22 PSV.dial :mixloop @15:51:55.45 CTV.dial :air,avoiding: :intake @15:51:55.46 PTV.dial :PRVmixing @15:51:55.50 PS.seek 0ml @15:52:07.42 delay 10 seconds @15:52:17.46 ATV.dial :air2 @15:52:18.40 AS.seek 0.329ml @15:52:23.26
PSV.dial PSR @15:52:24.55 Pulling 1.5ml of PSR.flush @15:52:24.56 PSR.select :flush @15:52:24.70 ATV.dial :waste2 @15:52:24.77
PS.seek 1.5ml @15:52:25.32 AS.reconfigure ASnorm @15:52:25.38 AS.seek :empty @15:52:41.80
delay 5 seconds @15:52:42.39 Lysate primed! @15:52:42.40 AFV.select :holdingCoil @15:52:42.43 ATV.dial :air2 @15:52:43.09 AS.reconfigure ASslow1 @15:52:43.15 AS.seek 0.069ml @15:52:46.82
PSV.dial :waste1 @15:52:47.47 PS.seek 0ml @15:52:57.50 delay 3 seconds @15:53:00.53 ATV.dial :AIV @15:53:01.43 AS.seek 0.469ml @15:53:04.47
PSV.dial PSR @15:53:05.01 PSR.select :air @15:53:05.16 PS.seek 2.5ml @15:53:32.61 PRV.dial CBV @15:53:32.64
CBV.dial PRV @15:53:32.66 PSV.dial :mixloop @15:53:33.89 CTV.dial :air,avoiding: :intake @15:53:33.90 PTV.dial :PRVmixing @15:53:33.94 PS.seek 0ml @15:54:01.29 PSV.dial PSR @15:54:02.57 Pulling 1.5ml of PSR.flush @15:54:02.58 PSR.select :flush @15:54:02.73 PS.seek 1.5ml @15:54:19.58 delay 5 seconds @15:54:22.14 delay 5 seconds @15:54:24.60
PSV.dial :waste1 @15:54:25.13 PS.seek 0ml @15:54:27.21 Solid Phase Extraction Heater at 49.3C @15:54:27.22 ATV.dial ASV @15:54:27.66 ASV.dial :SPEdelivery @15:54:27.75 AS.seek 0.041ml @15:54:41.76
PSV.dial PSR @15:54:42.29 PSR.select :air @15:54:42.43 PS.seek 2.5ml @15:55:09.52 PRV.dial CBV @15:55:09.55
CBV.dial PRV @15:55:09.57 PSV.dial :mixloop @15:55:10.77 CTV.dial :air,avoiding: :intake @15:55:10.79 PTV.dial :PRVmixing @15:55:10.82 PS.seek 0ml @15:55:37.76 PSV.dial PSR @15:55:39.00 Pulling 1.5ml of PSR.flush @15:55:39.01 PSR.select :flush @15:55:39.16 PS.seek 1.5ml @15:55:54.11 delay 10 seconds @15:55:55.58
delay 5 seconds @15:56:00.61 PSV.dial :waste1 @15:56:01.13 PS.seek 0ml @15:56:04.14 rinseLysateLineINT @15:56:04.15 AFV.select :carrierSolution @15:56:04.27 delay 2 seconds @15:56:06.29 AS.reconfigure ASconfig @15:56:06.34 AS.seek 1ml @15:56:17.60
PSV.dial PSR @15:56:18.13 PSR.select :air @15:56:18.27 PS.seek 2.5ml @15:56:45.13 PRV.dial CBV @15:56:45.55
CBV.dial PRV @15:56:45.64 PSV.dial :mixloop @15:56:46.82 CTV.dial :air,avoiding: :intake @15:56:46.84 PTV.dial :PRVmixing @15:56:46.87 PS.reconfigure PSslow @15:56:46.95 PS.seek 1.2ml @15:57:01.75 delay 1 second @15:57:02.77 AFV.select :holdingCoil @15:57:02.88 delay 1 second @15:57:03.90 ATV.dial AIV @15:57:04.45 AIV.dial :coilToMFB @15:57:04.47 AWV.select :waste @15:57:04.59 AS.reconfigure ASslow0 @15:57:04.66 AS.seek 0ml @15:57:13.89
PSV.dial :waste1 @15:57:15.39 PS.reconfigure PSconfig @15:57:15.46 PS.seek 0ml @15:57:28.66 PSV.dial :air @15:57:29.85 PS.seek 2.5ml @15:57:56.45 PSV.dial :mixloop @15:57:56.99 PS.reconfigure PSslow @15:57:57.05 PS.seek 0ml @15:58:45.55 ATV.dial :air2 @15:58:46.26 AS.reconfigure ASslow1 @15:58:46.32 AS.seek 0.05ml @15:58:48.32
PSV.dial :air @15:58:48.85 PS.reconfigure PSconfig @15:58:48.91 PS.seek 2.5ml @15:58:56.67 ATV.dial :AIV @15:58:57.55 AS.seek 0.01ml @15:59:05.86 ATV.dial :waste @15:59:07.59 AS.seek :empty @15:59:10.00 ethanol wash @15:59:10.01 ATV.dial :air2 @15:59:11.33 AS.reconfigure ASslow2 @15:59:11.39 AS.seek 0.009ml @15:59:15.60
PSV.dial :mixloop @15:59:16.13 PS.reconfigure PSslow @15:59:16.19 PS.seek 0ml @15:59:21.70 delay 3 seconds @15:59:24.73 Pulling 0.4ml of Ethanol Wash @15:59:24.74 ATV.dial :ethanol @15:59:25.59 AS.reconfigure ASslow1 @15:59:25.65 AS.seek 0.409ml @16:00:07.24
Cleaning Collection Syringe CSV.dial CSR @16:00:07.85 Pulling 4ml of CSR.flush @16:00:07.86 CSR.select :flush @16:00:08.01 CS.seek 4ml @16:00:19.42 delay 5 seconds @16:00:24.44 CSR.select :air @16:00:24.58 CS.seek 10ml @16:00:41.17 CSV.dial CTV @16:00:41.19
CTV.dial CSV @16:00:42.82 CBV.dial :waste1 @16:00:43.39 CS.seek 0ml @16:00:46.40 delay 3 seconds @16:00:49.43 ATV.dial :air1 @16:00:49.84 delay 3 seconds @16:00:52.92 AS.seek 0.434ml @16:00:58.15 delay 3 seconds @16:01:01.18 ATV.dial :ethanol @16:01:01.82 AS.seek 0.419ml @16:01:05.08 delay 3 seconds @16:01:08.11 ATV.dial ASV @16:01:09.47 AS.seek 0.092ml @16:01:10.67
CSV.dial CSR @16:01:11.27 Pulling 4ml of CSR.flush @16:01:11.28 CSR.select :flush @16:01:11.43 CS.seek 4ml @16:01:22.89 delay 5 seconds @16:01:27.91 CSR.select :air @16:01:28.05 CS.seek 10ml @16:01:44.54 CSV.dial CTV @16:01:44.58
CTV.dial CSV @16:01:45.12 CBV.dial :waste1 @16:01:45.14 CS.seek 0ml @16:02:12.00 Garaging FlushPuck in Collection Clamp @16:02:12.55 Elbow.reconfigure TightElbow @16:02:12.60 Elbow.seek CC @16:02:12.85 Elbow.reconfigure LooseElbow @16:02:12.96 Forearm.seek CC @16:02:14.02 Hand.close @16:02:14.82 CC.seek :open @16:02:15.48 delay 3 seconds @16:02:18.51 ATV.dial :waste2 @16:02:19.22
Forearm.seek :retract @16:02:19.31 AS.reconfigure ASconfig @16:02:19.37 AS.seek :empty @16:02:20.35
Elbow.reconfigure TightElbow @16:02:20.41 Elbow.seek :garage @16:02:23.48 Elbow.reconfigure LooseElbow @16:02:23.60 Forearm.reconfigure TightForearm @16:02:23.66 Forearm.seek :garage @16:02:24.85 Hand.open @16:02:25.02 ATV.dial :air2 @16:02:25.45 AS.reconfigure ASslow1 @16:02:25.51 AS.seek 1ml @16:02:25.64
Forearm.reconfigure LooseForearm @16:02:25.71 Forearm.seek :retract @16:02:26.77 Whole Cell Archive sampling 100ml @16:02:26.78 Loading wcr puck from tube 2 into Collection Clamp @16:02:27.04 SC.reconfigure SCtight @16:02:27.10 SC.seek 2 @16:02:29.30 SC.reconfigure SCloose @16:02:29.52 Elbow.reconfigure TightElbow @16:02:29.59 Elbow.seek 2 @16:02:31.64 Forearm.reconfigure TightForearm @16:02:31.71 Forearm.seek 2 + 700 counts @16:02:32.83 SE.reconfigure SEpick @16:02:32.92 SE.seek beamBreak @16:02:34.56 SE.configure SEconfig @16:02:34.73 Elbow.seek 2 @16:02:34.92 Forearm.seek 2 + 300 counts @16:02:35.24 Forearm.reconfigure LooseForearm @16:02:35.31 Hand.close @16:02:36.08 SE.seek :down @16:02:36.29 Forearm.seek :retract @16:02:37.27 Elbow.seek CC @16:02:37.49 SE.coast @16:02:38.94 Elbow.reconfigure LooseElbow @16:02:39.05 Forearm.reconfigure TightForearm @16:02:39.12 Forearm.seek CC @16:02:40.45 CC.seek :closed @16:02:44.72 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.30 @16:02:44.73 Hand.open @16:02:45.64 Forearm.reconfigure LooseForearm @16:02:45.71 Forearm.seek :retract @16:02:46.76 SC.coast @16:02:46.81 collectSample 100ml w/bubble point of 55psi @16:02:46.84 Priming 7ml @16:02:46.86 Gate.power :sampler,:ON @16:02:46.89 Intake.select :close @16:02:46.91 Exhaust.select :close @16:02:46.94 SS.hold @16:02:47.16 Calibrating pressure sensors @16:02:47.17 CTV.dial :air @16:02:48.72 SSV.dial :spit,avoiding: :exhaust @16:02:48.75 SS.reconfigure SSfast @16:02:48.82 SS.seek 5ml @16:02:51.54 SSV.dial CBV @16:02:51.57 CBV.dial SSV @16:02:53.20 delay 3.8 seconds @16:02:57.11 SSV.dial :spit @16:02:58.62 SS.seek 0ml @16:03:01.33 SSV.dial CBV @16:03:02.86 CTV.dial :intake @16:03:03.74 Waiting for external sampler to finish priming... @16:03:03.76 delayUntil "16:07:46.88" @16:05:47.08 delay 5 seconds @16:05:52.11 ATV.dial ASV @16:05:53.14 ASV.dial :SPEdelivery @16:05:53.17 AS.seek 0.02ml @16:07:46.90 SSV.dial CBV @16:07:46.92 CBV.dial SSV @16:07:46.95 Allowing 40s for 7.0ml of remaining 7.0ml @16:07:46.98 Intake.select :open @16:07:49.16 Intake @15.5psia, vacuum from 9.0 to 13.0psi @16:07:49.18 SS.reconfigure SSinhale.with maxSpeed: 150 @16:07:49.26 SS.seek 7ml @16:07:55.95 Stroke completed -- waiting for pressure to equalize ... @16:08:14.20 Intake.select :close @16:08:14.35 SSV.dial :exhaust @16:08:15.23 Exhaust.select :open @16:08:15.36 SS.reconfigure SSexhale @16:08:15.42 SS.seek 0ml @16:08:19.54 Exhaust.select :close @16:08:19.68 Sampling 100.0ml @16:08:19.71 Intake.select :close @16:08:19.72 Exhaust.select :close @16:08:19.76 SS.reconfigure SSconfig @16:08:19.83 SS.hold @16:08:20.04 CTV.dial :intake @16:08:20.06 SSV.dial CBV @16:08:20.10 CBV.dial SSV @16:08:21.01 Allowing 130s for 25.0ml of remaining 100.0ml @16:08:21.02 Intake.select :open @16:08:23.20 Intake @15.5psia, vacuum from 9.0 to 13.0psi @16:08:23.22 SS.reconfigure SSinhale.with maxSpeed: 150 @16:08:23.30 SS.seek 25ml @16:08:27.44 Vacuum too high -- waiting up to 40s @16:08:35.35 Intake @15.8psia, vacuum from 9.3 to 13.3psi @16:08:35.37 SS.reconfigure SSinhale.with maxSpeed: 135 @16:08:35.45 SS.seek 25ml @16:08:38.72 Vacuum too high -- waiting up to 40s @16:08:45.97 Intake @15.8psia, vacuum from 9.3 to 13.3psi @16:08:45.99 SS.reconfigure SSinhale.with maxSpeed: 122 @16:08:46.07 SS.seek 25ml @16:08:49.32 Vacuum too high -- waiting up to 40s @16:08:56.32 Intake @15.8psia, vacuum from 9.3 to 13.3psi @16:08:56.34 SS.reconfigure SSinhale.with maxSpeed: 110 @16:08:56.41 SS.seek 25ml @16:09:00.16 Vacuum too high -- waiting up to 40s @16:09:08.33 Intake @15.8psia, vacuum from 9.3 to 13.3psi @16:09:08.35 SS.reconfigure SSinhale.with maxSpeed: 99 @16:09:08.42 SS.seek 25ml @16:09:10.54 delay 5 seconds @16:09:12.63 Vacuum too high -- waiting up to 40s @16:09:15.64 ATV.dial :air2 @16:09:16.73 AS.seek 0.52ml @16:09:20.96 SS.reconfigure SSinhale.with maxSpeed: 89 @16:09:21.03 SS.seek 25ml @16:09:25.04 Stroke completed -- waiting for pressure to equalize ... @16:09:36.10 Intake.select :close @16:09:36.26 SSV.dial :exhaust @16:09:37.15 Exhaust.select :open @16:09:37.28 SS.reconfigure SSexhale @16:09:37.34 SS.seek 0ml @16:09:46.92 Exhaust.select :close @16:09:47.05 SSV.dial CBV @16:09:47.07 CBV.dial SSV @16:09:47.98 Allowing 130s for 25.0ml of remaining 75.0ml @16:09:48.00 Intake.select :open @16:09:50.18 Intake @15.6psia, vacuum from 9.1 to 13.1psi @16:09:50.20 SS.reconfigure SSinhale.with maxSpeed: 89 @16:09:50.28 SS.seek 25ml @16:09:54.57 Vacuum too high -- waiting up to 40s @16:10:00.68 Intake @15.8psia, vacuum from 9.3 to 13.3psi @16:10:00.70 SS.reconfigure SSinhale.with maxSpeed: 80 @16:10:00.78 SS.seek 25ml @16:10:04.28 Vacuum too high -- waiting up to 40s @16:10:10.16 SS.reconfigure SSinhale.with maxSpeed: 72 @16:10:10.23 SS.seek 25ml @16:10:14.50 Vacuum too high -- waiting up to 40s @16:10:21.41 Intake @15.8psia, vacuum from 9.3 to 13.3psi @16:10:21.43 SS.reconfigure SSinhale.with maxSpeed: 65 @16:10:21.51 SS.seek 25ml @16:10:26.44 Vacuum too high -- waiting up to 40s @16:10:33.89 Intake @15.7psia, vacuum from 9.2 to 13.2psi @16:10:33.91 SS.reconfigure SSinhale.with maxSpeed: 59 @16:10:33.99 SS.seek 25ml @16:10:39.41 Vacuum too high -- waiting up to 40s @16:10:47.12 Intake @15.8psia, vacuum from 9.3 to 13.3psi @16:10:47.14 SS.reconfigure SSinhale.with maxSpeed: 53 @16:10:47.21 SS.seek 25ml @16:10:54.47 Vacuum too high -- waiting up to 40s @16:10:57.59 delay 5 seconds @16:11:02.62 ATV.dial ASV @16:11:03.81 Intake @15.8psia, vacuum from 9.3 to 13.3psi @16:11:03.83 ASV.dial :wasteR @16:11:03.87 SS.reconfigure SSinhale.with maxSpeed: 48 @16:11:03.98 SS.seek 25ml @16:11:04.29 AS.seek 0.3ml @16:11:06.34 Stroke completed -- waiting for pressure to equalize ... @16:11:12.53 Intake.select :close @16:11:12.69 SSV.dial :exhaust @16:11:13.58 Exhaust.select :open @16:11:13.71 SS.reconfigure SSexhale @16:11:13.78 SS.seek 0ml @16:11:22.90 Exhaust.select :close @16:11:23.02 SSV.dial CBV @16:11:23.04 CBV.dial SSV @16:11:23.93 Allowing 130s for 25.0ml of remaining 50.0ml @16:11:23.95 Intake.select :open @16:11:26.13 Intake @15.6psia, vacuum from 9.1 to 13.1psi @16:11:26.15 SS.reconfigure SSinhale.with maxSpeed: 48 @16:11:26.23 SS.seek 25ml @16:11:34.91 Vacuum too high -- waiting up to 40s @16:11:42.36 Intake @15.7psia, vacuum from 9.2 to 13.2psi @16:11:42.38 SS.reconfigure SSinhale.with maxSpeed: 43 @16:11:42.46 SS.seek 25ml @16:11:48.78 delay 20 seconds @16:11:52.94 Vacuum too high -- waiting up to 40s @16:12:02.26 Intake @15.7psia, vacuum from 9.2 to 13.2psi @16:12:02.28 SS.reconfigure SSinhale.with maxSpeed: 39 @16:12:02.36 SS.seek 25ml @16:12:09.27 AS.seek 0.07ml @16:12:12.91 Vacuum too high -- waiting up to 40s @16:12:21.31 Intake @15.7psia, vacuum from 9.2 to 13.2psi @16:12:21.33 SS.reconfigure SSinhale.with maxSpeed: 35 @16:12:21.40 SS.seek 25ml @16:12:38.00 Stroke completed -- waiting for pressure to equalize ... @16:12:54.54 Intake.select :close @16:12:54.69 SSV.dial :exhaust @16:12:55.56 Exhaust.select :open @16:12:55.69 SS.reconfigure SSexhale @16:12:55.74 delay 20 seconds @16:12:55.81 SS.seek 0ml @16:13:05.32 Exhaust.select :close @16:13:05.45 SSV.dial CBV @16:13:05.47 CBV.dial SSV @16:13:06.38 Allowing 130s for 25.0ml of remaining 25.0ml @16:13:06.39 Intake.select :open @16:13:08.58 Intake @15.7psia, vacuum from 9.2 to 13.2psi @16:13:08.60 SS.reconfigure SSinhale.with maxSpeed: 35 @16:13:08.68 SS.seek 25ml @16:13:15.79 ATV.dial :waste2 @16:13:16.59 AS.reconfigure ASconfig @16:13:16.64 AS.seek :empty @16:13:21.03 Elution @16:13:21.04 ATV.dial :air1 @16:13:21.13 Vacuum too high -- waiting up to 40s @16:13:22.25 AS.reconfigure ASslow1 @16:13:22.32 AS.seek 0.049ml @16:13:27.42 Intake @15.6psia, vacuum from 9.1 to 13.1psi @16:13:27.44 SS.reconfigure SSinhale.with maxSpeed: 32 @16:13:27.52 SS.seek 25ml @16:13:32.73 delay 3 seconds @16:13:35.76 ATV.dial :ElutionWater @16:13:36.47 AS.seek 0.109ml @16:13:41.22 Vacuum too high -- waiting up to 40s @16:13:48.71 Intake @15.8psia, vacuum from 9.3 to 13.3psi @16:13:48.73 SS.reconfigure SSinhale.with maxSpeed: 29 @16:13:48.83 SS.seek 25ml @16:13:48.86 delay 3 seconds @16:13:51.88 ATV.dial ASV,via: :air2 @16:13:54.09 ASV.dial :SPEelution @16:13:54.70 AFV.select :carrierSolution @16:13:54.87 AS.reconfigure ASnorm @16:13:54.92 AS.seek 0.759ml @16:14:17.44 Vacuum too high -- waiting up to 40s @16:14:28.14 delay 3 seconds @16:14:28.42 SS.reconfigure SSinhale.with maxSpeed: 26 @16:14:28.49 SS.seek 25ml @16:14:31.15 AFV.select :holdingCoil @16:14:31.32 AS.reconfigure ASslow1 @16:14:31.38 AS.seek 0.474ml @16:14:33.46 Stroke completed -- waiting for pressure to equalize ... @16:14:41.46 Intake.select :close @16:14:41.62 SSV.dial :exhaust @16:14:42.51 Exhaust.select :open @16:14:42.64 SS.reconfigure SSexhale @16:14:42.70 SS.seek 0ml @16:14:52.17 Exhaust.select :close @16:14:52.31 Sampled 100.0ml @16:14:52.33 Drying puck before fixing for 4:00 @16:14:52.34 CBV.dial :waste2b @16:14:53.27 delay 1:00 @16:14:55.32 Gate.power :sampler,:OFF @16:15:28.94 delay 5 seconds @16:15:34.03 AS.reconfigure ASslow2 @16:15:34.09 AS.seek 0.439ml @16:15:53.30 CSV.dial :air @16:15:53.82 CS.reconfigure CSfast @16:15:53.89 CS.seek 10ml @16:16:09.33 delay 1:00 @16:16:21.01 Pumping wcr puck from tube 2 up to 55psi... @16:16:21.03 CBV.dial SSV @16:16:22.49 CBV.dial between CSV and waste2b @16:16:23.64 CSV.dial between air and CTV @16:16:24.03 CS.seek 7.231ml @16:16:32.15 CTV.dial CSV,avoiding: :air @16:16:32.18 CSV.dial CTV @16:16:33.09 CS.reconfigure CSpressurize @16:16:33.16 CS.seek 0ml @16:16:49.19 CBV.dial :waste2b @16:16:49.60 CS.reconfigure CSconfig @16:16:49.66 CS.seek :empty @16:16:54.08 delay 1:00 @16:17:09.36 pushing eluate to holding coil @16:17:09.44 AS.seek 0.279ml @16:17:54.11 CTV.dial :air,avoiding: :intake @16:17:55.66 Fix #1 of 2 (with rnal) for 2:00 @16:17:55.68 CSV.dial CSR @16:17:56.24 Pulling 2ml of CSR.rnal @16:17:56.25 CSR.select :rnal @16:17:56.40 CS.seek 2ml @16:18:02.68 delay 5 seconds @16:18:07.70 CSR.select :air @16:18:07.83 CS.reconfigure CSfast @16:18:07.90 CS.seek 10ml @16:18:29.59 Pumping wcr puck from tube 2 up to 55psi... @16:18:29.60 CBV.dial SSV @16:18:31.53 CBV.dial between CSV and waste2b @16:18:32.69 CSV.dial between air and CTV @16:18:33.41 CS.seek 7.118ml @16:18:41.93 CTV.dial CSV,avoiding: :air @16:18:41.95 CSV.dial CTV @16:18:43.56 CS.reconfigure CSpressurize @16:18:43.63 CS.seek 0ml @16:19:01.50 delay 2:00 @16:19:50.45 delay 30 seconds @16:20:20.49 ATV.dial :waste,via: :air3 @16:20:22.26 AS.reconfigure ASconfig @16:20:22.31 AS.seek :empty @16:20:38.69 Parking Bleach in LysateSource line and SPEcolumn @16:20:38.70 ATV.dial :air3 @16:20:39.57 AS.reconfigure ASslow1 @16:20:39.63 AS.seek 0.029ml @16:20:46.01 ATV.dial :ElutionWater @16:20:46.65 AS.seek 0.059ml @16:20:52.94 ATV.dial :air3 @16:20:53.57 AS.seek 0.069ml @16:20:55.86 delay 2 seconds @16:20:57.89 ATV.dial :bleach @16:20:58.37 AS.seek 0.769ml @16:21:01.53 CBV.dial :waste2b @16:21:01.93 CS.reconfigure CSconfig @16:21:02.00 CS.seek :empty @16:21:04.27 delay 1:00 @16:22:04.31 CTV.dial :air,avoiding: :intake @16:22:05.87 Fix #2 of 2 (with rnal) for 2:00 @16:22:05.88 CSV.dial CSR @16:22:06.45 Pulling 2ml of CSR.rnal @16:22:06.46 CSR.select :rnal @16:22:06.61 CS.seek 2ml @16:22:12.81 delay 5 seconds @16:22:17.84 CSR.select :air @16:22:17.97 CS.reconfigure CSfast @16:22:18.04 CS.seek 10ml @16:22:39.62 Pumping wcr puck from tube 2 up to 55psi... @16:22:39.63 CBV.dial SSV @16:22:41.08 CBV.dial between CSV and waste2b @16:22:42.22 CSV.dial between air and CTV @16:22:42.94 CS.seek 7.057ml @16:22:51.87 CTV.dial CSV,avoiding: :air @16:22:51.89 CSV.dial CTV @16:22:53.53 CS.reconfigure CSpressurize @16:22:53.60 CS.seek 0ml @16:23:10.07 delay 2:00 @16:23:19.37 delay 3 seconds @16:23:22.40 ATV.dial :air3 @16:23:22.97 delay 3 seconds @16:23:26.05 AS.seek 0.794ml @16:23:31.37 delay 3 seconds @16:23:34.40 ATV.dial :bleach @16:23:35.03 AS.seek 0.779ml @16:23:38.34 delay 3 seconds @16:23:41.37 ATV.dial :AIV @16:23:42.66 AS.reconfigure ASconfig @16:23:42.71 AS.seek 0.015ml @16:24:26.95 ATV.dial :waste @16:24:28.51 AS.seek :empty @16:24:29.72 ATV.dial :air3 @16:24:30.88 AS.reconfigure ASslow1 @16:24:30.95 AS.seek 0.009ml @16:24:33.25 delay 2 seconds @16:24:35.28 ATV.dial :bleach @16:24:35.76 AS.seek 0.579ml @16:25:10.10 CBV.dial :waste2b @16:25:10.48 CS.reconfigure CSconfig @16:25:10.55 CS.seek :empty @16:25:14.05 delay 1:00 @16:26:14.09 CTV.dial :air,avoiding: :intake @16:26:15.68 Storing wcr puck in Collection Clamp to tube 1 @16:26:15.87 SC.reconfigure SCtight @16:26:15.93 SC.seek 1 @16:26:18.08 SC.reconfigure SCloose @16:26:18.30 Elbow.reconfigure TightElbow @16:26:18.37 Elbow.seek 1 @16:26:20.30 Forearm.reconfigure TightForearm @16:26:20.37 Forearm.seek 1 + 700 counts @16:26:21.42 SE.reconfigure SEpick @16:26:21.51 SE.seek beamBreak @16:26:24.33 SE.configure SEconfig @16:26:24.50 Elbow.reconfigure LimpElbow @16:26:24.56 Forearm.reconfigure LooseForearm @16:26:24.62 Forearm.seek 1 @16:26:24.97 Elbow.hold @16:26:25.05 Elbow.reconfigure TightElbow @16:26:25.16 SE.seek 4.06 Pucks below Top Plate @16:26:25.72 Forearm.force -20 @16:26:25.77 Forearm.seek 1 + 339 counts @16:26:26.09 Forearm.reconfigure TightForearm @16:26:26.16 Forearm.seek 1 + 700 counts @16:26:26.37 SE.seek 5.06 Pucks below Top Plate @16:26:27.06 Forearm.reconfigure LooseForearm @16:26:27.13 Forearm.seek :retract @16:26:28.08 Elbow.seek CC @16:26:29.77 Elbow.reconfigure LooseElbow @16:26:29.89 Forearm.seek CC @16:26:30.66 delay 3 seconds @16:26:30.91 Hand.close @16:26:31.72 CC.seek :open @16:26:33.69 ATV.dial :air3 @16:26:34.26 delay 3 seconds @16:26:36.22 Forearm.seek :retract @16:26:37.25 Elbow.reconfigure TightElbow @16:26:37.31 Elbow.seek 1 @16:26:37.42 AS.seek 0.604ml @16:26:39.26 Elbow.reconfigure LimpElbow @16:26:39.32 Forearm.seek 1 @16:26:40.86 Elbow.hold @16:26:40.95 Elbow.reconfigure TightElbow @16:26:41.02 Hand.open @16:26:41.96 Forearm.force -20 @16:26:42.01 Forearm.seek 1 + 346 counts @16:26:42.33 Forearm.reconfigure TightForearm @16:26:42.40 Forearm.seek 1 + 700 counts @16:26:42.65 delay 3 seconds @16:26:42.76 SE.seek :down @16:26:43.89 Forearm.reconfigure LooseForearm @16:26:43.95 Forearm.seek :retract @16:26:45.14 SE.coast @16:26:45.19 SC.coast @16:26:45.25 Starting finalPurge @16:26:45.26 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:26:45.36 Hand.open @16:26:45.71 Elbow.seek FlushPuck::Garage @16:26:45.77 ATV.dial :bleach @16:26:46.42 AS.seek 0.589ml @16:26:47.81
Elbow.reconfigure LooseElbow @16:26:47.93 Forearm.seek :garage @16:26:48.95 Hand.close @16:26:49.73 delay 3 seconds @16:26:49.89
Forearm.seek :retract @16:26:50.94 Elbow.reconfigure TightElbow @16:26:51.00 Elbow.seek CC @16:26:52.77 ATV.dial :ASV @16:26:53.82 ASV.dial :wasteR @16:26:53.94
Elbow.reconfigure LooseElbow @16:26:54.05 Forearm.reconfigure TightForearm @16:26:54.11 Forearm.seek CC @16:26:54.48 AS.seek 0ml @16:26:55.46
CC.seek :closed @16:26:59.76 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.34 @16:26:59.85 Hand.open @16:27:00.64 Forearm.reconfigure LooseForearm @16:27:00.71 Forearm.seek :retract @16:27:01.75 Flushing the Collection Valve::Manifold @16:27:01.89 CSV.dial CSR @16:27:02.44 Pulling 5ml of CSR.flush @16:27:02.45 CSR.select :flush @16:27:02.60 CS.seek 5ml @16:27:16.62 delay 5 seconds @16:27:21.64 CSR.select :air @16:27:21.82 CS.reconfigure CSfast @16:27:21.88 CS.seek 7.5ml @16:27:29.32 CSV.dial :waste @16:27:30.86 CS.reconfigure CSconfig @16:27:30.93 CS.seek 0ml @16:27:51.40 Flushing lower manifold's waste1 port @16:27:51.54 CSV.dial :flush @16:27:52.08 CS.reconfigure CSfast @16:27:52.15 CS.seek 5ml @16:28:06.24 delay 5 seconds @16:28:11.27 CSV.dial :air @16:28:11.98 CS.seek 7ml @16:28:18.34 CSV.dial CTV @16:28:18.37
CTV.dial CSV @16:28:19.99 CBV.dial :waste1 @16:28:20.92 CS.seek 0ml @16:28:40.09 Flushing lower manifold's waste2b port @16:28:40.23 CSV.dial :flush @16:28:41.22 CS.seek 5ml @16:28:53.23 delay 1:00 @16:28:55.14
delay 5 seconds @16:29:00.17 CSV.dial :air @16:29:00.75 CS.seek 7ml @16:29:06.90 CSV.dial CTV @16:29:06.93
CTV.dial CSV @16:29:07.44 CBV.dial :waste2b @16:29:08.34 CS.seek 0ml @16:29:27.47 Flushing lower manifold's waste1 port @16:29:27.48 CSV.dial CSR @16:29:28.06 Pulling 4ml of CSR.mfbkill @16:29:28.07 CSR.select :mfbkill @16:29:28.22 CS.reconfigure CSconfig @16:29:28.28 CS.seek 4ml @16:29:39.65 delay 5 seconds @16:29:44.67 CSR.select :air @16:29:44.80 CS.reconfigure CSfast @16:29:44.87 CS.seek 10ml @16:29:53.25 AFV.select :carrierSolution @16:29:53.37 delay 2 seconds @16:29:55.39 AS.reconfigure ASconfig @16:29:55.45 AS.seek 1ml @16:30:01.44 CSV.dial CTV @16:30:01.47
CTV.dial CSV @16:30:02.01 CBV.dial :waste1 @16:30:02.91 CS.seek 0ml @16:30:29.75 Flushing lower manifold's waste2b port @16:30:29.76 CSV.dial CSR @16:30:30.36 Pulling 4ml of CSR.mfbkill @16:30:30.37 CSR.select :mfbkill @16:30:30.52 CS.reconfigure CSconfig @16:30:30.58 CS.seek 4ml @16:30:42.03 delay 5 seconds @16:30:47.05 CSR.select :air @16:30:47.18 CS.reconfigure CSfast @16:30:47.25 CS.seek 10ml @16:30:53.20 delay 1 second @16:30:54.22 AFV.select :holdingCoil @16:30:54.34 delay 1 second @16:30:55.36 ATV.dial ASV @16:30:55.37 ASV.dial :wasteR @16:30:55.40 AS.reconfigure ASslow1 @16:30:55.47 AS.seek 0ml @16:31:03.69 CSV.dial CTV @16:31:03.72
CTV.dial CSV @16:31:04.26 CBV.dial :waste2b @16:31:05.17 CS.seek 0ml @16:31:32.03 Killing upper manifold @16:31:32.04 CSV.dial CSR @16:31:32.64 Pulling 4ml of CSR.mfbkill @16:31:32.65 CSR.select :mfbkill @16:31:32.80 CS.reconfigure CSconfig @16:31:32.86 CS.seek 4ml @16:31:44.50 delay 5 seconds @16:31:49.52 CSR.select :air @16:31:49.65 CS.reconfigure CSfast @16:31:49.72 CS.seek 10ml @16:32:06.25 CSV.dial CBV @16:32:06.28
CBV.dial CSV @16:32:07.52 CTV.dial :air @16:32:09.08 CS.seek 0ml @16:32:35.93 delay 7 seconds @16:32:42.96 CS.seek 3ml @16:32:52.21 CSV.dial :waste @16:32:52.73 CS.seek 0ml @16:33:01.41 Flushing upper manifold @16:33:01.54 CSV.dial :flush @16:33:02.06 CS.seek 5ml @16:33:16.02 delay 5 seconds @16:33:21.05 CSV.dial :air @16:33:21.64 CS.seek 7ml @16:33:27.75 CSV.dial CBV @16:33:27.78
CBV.dial CSV @16:33:29.02 CTV.dial :air @16:33:29.04 CS.seek 0ml @16:33:48.08 delay 7 seconds @16:33:55.11 CS.seek 3ml @16:34:03.80 CSV.dial :waste @16:34:04.34 CS.seek 0ml @16:34:13.13 CSV.dial :flush @16:34:13.66 CS.seek 3ml @16:34:16.87 delay 20 seconds @16:34:22.37
delay 5 seconds @16:34:27.40 CSV.dial :air @16:34:27.98 CS.seek 5ml @16:34:34.21 CSV.dial :air @16:34:34.24 CS.seek 0ml @16:34:36.90 ATV.dial :air3 @16:34:38.43 AS.seek 0.59ml @16:34:48.05
delay 7 seconds @16:34:55.08 CS.seek 3ml @16:35:03.80 CSV.dial :waste @16:35:04.69 CS.seek 0ml @16:35:13.38 Flushing lower manifold's waste1 port @16:35:13.51 CSV.dial :flush @16:35:14.05 CS.seek 5ml @16:35:28.12 delay 5 seconds @16:35:33.15 CSV.dial :air @16:35:33.73 CS.seek 7ml @16:35:39.97 CSV.dial CTV @16:35:40.00
CTV.dial CSV @16:35:41.60 CBV.dial :waste1 @16:35:42.16 CS.seek 0ml @16:36:01.03 Flushing lower manifold's waste2b port @16:36:01.16 CSV.dial :flush @16:36:02.03 CS.seek 5ml @16:36:15.87 delay 5 seconds @16:36:20.90 CSV.dial :air @16:36:21.50 CS.seek 7ml @16:36:27.62 CSV.dial CTV @16:36:27.65
CTV.dial CSV @16:36:28.17 CBV.dial :waste2b @16:36:29.06 CS.seek 0ml @16:36:37.43 delay 3 seconds @16:36:40.46 ATV.dial :ASV @16:36:41.90 ASV.dial :wasteR @16:36:41.99 AS.seek 0.09ml @16:36:47.94
Garaging FlushPuck in Collection Clamp @16:36:48.03 Elbow.reconfigure TightElbow @16:36:48.09 Elbow.seek CC @16:36:48.36 Elbow.reconfigure LooseElbow @16:36:48.47 Forearm.seek CC @16:36:49.55 Hand.close @16:36:50.35 CC.seek :open @16:36:54.74 Forearm.seek :retract @16:36:55.81 Elbow.reconfigure TightElbow @16:36:55.87 Elbow.seek :garage @16:36:59.47 Elbow.reconfigure LooseElbow @16:36:59.58 Forearm.reconfigure TightForearm @16:36:59.65 Forearm.seek :garage @16:37:00.84 Hand.open @16:37:01.66 Forearm.reconfigure LooseForearm @16:37:01.72 Forearm.seek :retract @16:37:02.78 Starting initialPurge @16:37:02.79 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:37:02.88 Hand.open @16:37:03.15 Elbow.reconfigure TightElbow @16:37:03.21 Elbow.seek FlushPuck::Garage @16:37:03.34 Elbow.reconfigure LooseElbow @16:37:03.45 Forearm.seek :garage @16:37:04.53 Hand.close @16:37:05.34 Forearm.seek :retract @16:37:06.38 Elbow.reconfigure TightElbow @16:37:06.44 Elbow.seek CC @16:37:09.34 Elbow.reconfigure LooseElbow @16:37:09.45 Forearm.reconfigure TightForearm @16:37:09.50 Forearm.seek CC @16:37:10.84 CC.seek :closed @16:37:15.12 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.33 @16:37:15.20 Hand.open @16:37:16.03 Forearm.reconfigure LooseForearm @16:37:16.09 Forearm.seek :retract @16:37:17.16 Flushing the Collection Valve::Manifold @16:37:17.30 CSV.dial CSR @16:37:17.87 Pulling 5ml of CSR.flush @16:37:17.88 CSR.select :flush @16:37:18.03 CS.reconfigure CSconfig @16:37:18.09 CS.seek 5ml @16:37:32.00 delay 5 seconds @16:37:37.02 CSR.select :air @16:37:37.19 CS.reconfigure CSfast @16:37:37.25 CS.seek 7.5ml @16:37:44.77 CSV.dial :waste @16:37:46.42 CS.reconfigure CSconfig @16:37:46.48 CS.seek 0ml @16:38:06.65 Purging external Intake @16:38:06.79 CSV.dial :flush @16:38:07.32 CS.reconfigure CSfast @16:38:07.38 CS.seek 10ml @16:38:22.83 delay 30 seconds @16:38:34.21
delay 5 seconds @16:38:39.24 CSV.dial CBV @16:38:39.28
CBV.dial CSV @16:38:40.20 CTV.dial :intake @16:38:41.07 Intake.select :open @16:38:41.20 CS.seek 0ml @16:38:52.87 ATV.dial :extSrc @16:38:53.66 delay 3 seconds @16:38:56.68 ATV.dial :ElutionWater @16:38:57.85 AS.seek 0.12ml @16:39:04.09 ATV.dial :air3 @16:39:04.73 AS.seek 0.13ml @16:39:07.04 delay 2 seconds @16:39:09.07 ATV.dial :bleach @16:39:09.54 AS.seek 0.23ml @16:39:09.62
Intake.select :close @16:39:09.74 CTV.dial :air @16:39:10.61 Flushing upper manifold @16:39:11.14 CSV.dial :flush @16:39:12.10 CS.seek 5ml @16:39:25.99 delay 5 seconds @16:39:29.92 delay 3 seconds @16:39:31.02
CSV.dial :air @16:39:31.58 CS.seek 7ml @16:39:32.95 ATV.dial :air3 @16:39:33.52 delay 3 seconds @16:39:36.60 AS.seek 0.255ml @16:39:37.66 CSV.dial CBV @16:39:37.69
CBV.dial CSV @16:39:38.89 CTV.dial :air @16:39:38.91 CS.seek 0ml @16:39:41.90 delay 3 seconds @16:39:44.93 ATV.dial :bleach @16:39:45.57 AS.seek 0.24ml @16:39:48.86 delay 3 seconds @16:39:51.89 ATV.dial :ASV @16:39:52.93 ASV.dial :SPEdelivery @16:39:53.31 AFV.select :carrierSolution @16:39:53.43 delay 2 seconds @16:39:55.45 AS.reconfigure ASconfig @16:39:55.51 AS.seek 0.5ml @16:39:57.96
delay 7 seconds @16:40:04.99 CS.seek 3ml @16:40:10.74 delay 1 second @16:40:11.76 AFV.select :holdingCoil @16:40:11.91 AS.reconfigure ASslow1 @16:40:11.97 AS.seek 0.17ml @16:40:13.81
CSV.dial :waste @16:40:14.35 CS.seek 0ml @16:40:23.07 CSV.dial :flush @16:40:23.58 CS.seek 3ml @16:40:32.34 delay 5 seconds @16:40:37.37 CSV.dial :air @16:40:37.95 CS.seek 5ml @16:40:44.03 CSV.dial :air @16:40:44.06 CS.seek 0ml @16:40:57.93 delay 7 seconds @16:41:04.97 CS.seek 3ml @16:41:13.78 CSV.dial :waste @16:41:14.66 CS.seek 0ml @16:41:18.63 SPE.hold 2:46:40,95C @16:41:18.71 delay 3:00 @16:41:23.56
Garaging FlushPuck in Collection Clamp @16:41:23.65 Elbow.reconfigure TightElbow @16:41:23.71 Elbow.seek CC @16:41:23.94 Elbow.reconfigure LooseElbow @16:41:24.05 Forearm.seek CC @16:41:25.11 Hand.close @16:41:25.86 CC.seek :open @16:41:30.24 Forearm.seek :retract @16:41:31.30 Elbow.reconfigure TightElbow @16:41:31.36 Elbow.seek :garage @16:41:34.36 Elbow.reconfigure LooseElbow @16:41:34.47 Forearm.reconfigure TightForearm @16:41:34.53 Forearm.seek :garage @16:41:35.72 Hand.open @16:41:36.53 Forearm.reconfigure LooseForearm @16:41:36.59 Forearm.seek :retract @16:41:37.63 Completed [mbaPhase] begun 14:59:01 @16:41:37.65 Extending 190905totalPostcanqc until completion of MFBPCR @16:44:18.73 AFV.select :carrierSolution @16:44:18.85 delay 2 seconds @16:44:20.87 AS.reconfigure ASconfig @16:44:20.92 AS.seek 1ml @16:45:08.92 delay 1 second @16:45:09.94 AFV.select :holdingCoil @16:45:10.05 delay 1 second @16:45:11.07 ASV.dial :wasteD @16:45:12.26 AS.seek 0ml @16:46:10.07 Priming Eluate @16:46:10.08 AFV.select :carrierSolution @16:46:10.21 delay 2 seconds @16:46:12.23 AS.seek 0.5ml @16:46:41.28 delay 1 second @16:46:42.30 AFV.select :holdingCoil @16:46:42.41 ATV.dial ASV @16:46:42.42 ASV.dial :SPErecovery @16:46:43.04 Swish #1 of 2 @16:46:43.13 AS.reconfigure ASslow0 @16:46:43.19 AS.seek 0.6ml @16:46:53.57 AS.seek 0.5ml @16:47:03.90 Swish #2 of 2 @16:47:04.46 AS.seek 0.6ml @16:47:14.92 AS.seek 0.5ml @16:47:25.34 delay 3 seconds @16:47:28.42 AS.reconfigure ASslow1 @16:47:28.48 AS.seek 0.684ml @16:48:05.76 delay 3 seconds @16:48:08.79 Eluate primed @16:48:08.80 ATV.dial :waste2 @16:48:09.48 AS.reconfigure ASnorm @16:48:09.54 AS.seek 0ml @16:48:44.37 SPE.coast @16:48:44.45 ASV.dial :SPErecovery @16:48:44.48 Setting up PCR for Pp -- writing /var/log/esp/Pp19sep0516h100ml.pcr @16:48:44.50 Building PCR Slug from ASV with MM1 mix, PP2 primer @16:48:44.53 Priming with carrier @16:48:44.55 ATV.dial ARV @16:48:45.54 ARV.dial :carrier @16:48:45.91 AS.seek 0.5ml @16:49:11.47 AFV.select :carrierSolution @16:49:11.60 delay 2 seconds @16:49:13.62 AS.reconfigure ASconfig @16:49:13.69 AS.seek 1ml @16:49:42.70 delay 1 second @16:49:43.73 AFV.select :holdingCoil @16:49:43.83 delay 1 second @16:49:44.85 ATV.dial :waste @16:49:46.38 AS.reconfigure ASnorm @16:49:46.44 AS.seek :empty @16:50:37.18 AFV.select :carrierSolution @16:50:37.31 delay 2 seconds @16:50:39.33 AS.reconfigure ASconfig @16:50:39.40 AS.seek 0.5ml @16:51:08.51 delay 1 second @16:51:09.53 AFV.select :holdingCoil @16:51:09.63 Building Slug @16:51:09.64 ATV.dial :ARV @16:51:11.32 delay 2 seconds @16:51:13.34 ARV.dial :air1 @16:51:13.77 delay 2 seconds @16:51:15.84 AS.reconfigure ASslow2 @16:51:15.91 AS.seek 0.506ml @16:51:22.12 delay 2 seconds @16:51:24.15 ARV.dial :MM1 @16:51:24.87 delay 2 seconds @16:51:26.89 Adding MM1 @16:51:26.97 AS.seek 0.524ml @16:51:45.47 delay 25 seconds @16:52:10.50 ARV.dial :PP2 @16:52:10.91 Adding PP2 @16:52:10.92 delay 2 seconds @16:52:13.00 AS.seek 0.53ml @16:52:19.42 delay 10 seconds @16:52:29.46 ARV.dial :air2 @16:52:29.87 adding ARV air @16:52:29.88 delay 2 seconds @16:52:31.96 AS.seek 0.536ml @16:52:38.15 delay 5 seconds @16:52:43.18 ARV.dial :carrier @16:52:44.49 delay 7 seconds @16:52:51.57 AS.seek 0.594ml @16:53:50.01 delay 5 seconds @16:53:55.04 ATV.dial :ASV @16:53:55.45 delay 2 seconds @16:53:57.53 AS.seek 0.6ml @16:54:03.73 delay 4 seconds @16:54:07.76 ATV.dial ARV @16:54:08.30 delay 2 seconds @16:54:10.32 ARV.dial :carrier @16:54:10.33 delay 2 seconds @16:54:12.41 AS.seek 0.65ml @16:55:02.90 delay 4 seconds @16:55:06.93 ATV.dial PCR @16:55:07.54 ASV.dial :waste1 @16:55:08.35 ARV.dial :air1 @16:55:08.76 Positioning Slug by 0.324ml @16:55:08.85 AS.seek 0.326ml @17:00:34.90 delay 5 seconds @17:00:39.93 ATV.dial ARV @17:00:40.68 ASV.dial :PCRlock @17:00:41.08 delay 5 seconds @17:00:46.10 Running Pp Profile60C350 thermocycling @17:00:46.12 PCR.program Profile60C350 @17:00:46.27 Profile60C350 should complete in < 1:48:05 @17:00:46.28 PCR.run 2:20:06.25 @18:25:31.04 PCR.off @18:25:33.30 PCR for Pp completed -- closed /var/log/esp/Pp19sep0516h100ml.pcr @18:25:33.38 Cleaning PCR @18:25:33.39 AFV.select :carrierSolution @18:25:33.51 delay 2 seconds @18:25:35.53 AS.reconfigure ASconfig @18:25:35.59 AS.seek 0.5ml @18:25:45.90 delay 1 second @18:25:46.92 AFV.select :holdingCoil @18:25:47.03 ASV.dial :waste1 @18:25:47.43 ATV.dial :air3 @18:25:48.71 AS.reconfigure ASslow1 @18:25:48.78 AS.seek 0.51ml @18:25:51.06 delay 2 seconds @18:25:53.09 ATV.dial :bleach @18:25:53.72 AS.seek 0.81ml @18:26:54.30 delay 3 seconds @18:26:57.33 ATV.dial :air3 @18:26:57.89 delay 3 seconds @18:27:00.96 AS.seek 0.835ml @18:27:06.24 delay 3 seconds @18:27:09.27 ATV.dial :bleach @18:27:09.90 AS.seek 0.82ml @18:27:13.19 delay 3 seconds @18:27:16.22 ARV.dial :waste @18:27:16.84 delay 3 seconds @18:27:19.86 ATV.dial :ARV @18:27:20.75 AS.reconfigure ASconfig @18:27:20.82 AS.seek 0.47ml @18:27:41.24 AFV.select :carrierSolution @18:27:41.36 delay 2 seconds @18:27:43.38 AS.seek 1ml @18:28:14.13 delay 1 second @18:28:15.16 AFV.select :holdingCoil @18:28:15.27 delay 1 second @18:28:16.29 AS.reconfigure ASfast @18:28:16.35 AS.seek 0ml @18:28:43.69 AFV.select :carrierSolution @18:28:43.81 delay 2 seconds @18:28:45.83 AS.reconfigure ASconfig @18:28:45.89 AS.seek 1ml @18:29:43.68 delay 1 second @18:29:44.70 AFV.select :holdingCoil @18:29:44.81 delay 1 second @18:29:45.83 AS.reconfigure ASfast @18:29:45.89 AS.seek 0ml @18:30:13.26 AFV.select :carrierSolution @18:30:13.38 delay 2 seconds @18:30:15.40 AS.reconfigure ASconfig @18:30:15.46 AS.seek 1ml @18:31:13.21 delay 1 second @18:31:14.24 AFV.select :holdingCoil @18:31:14.35 delay 1 second @18:31:15.37 AS.reconfigure ASfast @18:31:15.43 AS.seek 0ml @18:31:42.77 ARV.dial between carrier and air1 @18:31:43.28 ATV.dial :air3 @18:31:44.57 AS.reconfigure ASslow1 @18:31:44.63 AS.seek 0.01ml @18:31:47.02 delay 2 seconds @18:31:49.05 ATV.dial :bleach @18:31:49.69 AS.seek 0.81ml @18:34:30.91 delay 3 seconds @18:34:33.94 ATV.dial :air3 @18:34:34.49 delay 3 seconds @18:34:37.57 AS.seek 0.835ml @18:34:42.87 delay 3 seconds @18:34:45.90 ATV.dial :bleach @18:34:46.52 AS.seek 0.82ml @18:34:49.85 delay 3 seconds @18:34:52.88 ATV.dial PCR @18:34:53.30 AS.reconfigure ASconfig @18:34:53.35 AS.seek :empty @18:35:40.82 PCR.program :bleachProfile @18:35:40.88 PCR program should complete in < 2:45 @18:35:40.89 PCR.run 10:00 @18:38:42.73 PCR.off @18:38:42.76 AFV.select :carrierSolution @18:38:43.38 delay 2 seconds @18:38:45.40 AS.seek 1ml @18:39:43.19 delay 1 second @18:39:44.21 AFV.select :holdingCoil @18:39:44.32 delay 1 second @18:39:45.34 AS.reconfigure ASfast @18:39:45.41 AS.seek 0ml @18:40:12.77 delay 3 seconds @18:40:15.80 AFV.select :carrierSolution @18:40:15.90 delay 2 seconds @18:40:17.92 AS.reconfigure ASconfig @18:40:17.98 AS.seek 1ml @18:41:15.72 delay 1 second @18:41:16.75 AFV.select :holdingCoil @18:41:16.85 delay 1 second @18:41:17.87 AS.reconfigure ASfast @18:41:17.93 AS.seek 0ml @18:41:45.30 delay 3 seconds @18:41:48.33 AFV.select :carrierSolution @18:41:48.43 delay 2 seconds @18:41:50.45 AS.reconfigure ASconfig @18:41:50.52 AS.seek 1ml @18:42:48.29 delay 1 second @18:42:49.31 AFV.select :holdingCoil @18:42:49.42 delay 1 second @18:42:50.44 AS.reconfigure ASfast @18:42:50.50 AS.seek 0ml @18:43:17.85 delay 3 seconds @18:43:20.88 AFV.select :carrierSolution @18:43:20.98 delay 2 seconds @18:43:23.00 AS.reconfigure ASconfig @18:43:23.06 AS.seek 1ml @18:44:20.82 delay 1 second @18:44:21.85 AFV.select :holdingCoil @18:44:21.95 delay 1 second @18:44:22.97 AS.reconfigure ASfast @18:44:23.03 AS.seek 0ml @18:44:50.38 delay 3 seconds @18:44:53.40 AFV.select :carrierSolution @18:44:53.51 delay 2 seconds @18:44:55.53 AS.reconfigure ASconfig @18:44:55.60 AS.seek 1ml @18:45:53.35 delay 1 second @18:45:54.37 AFV.select :holdingCoil @18:45:54.48 delay 1 second @18:45:55.50 AS.reconfigure ASnorm @18:45:55.56 AS.seek 0ml @18:46:46.29 Setting up PCR for Ss -- writing /var/log/esp/Ss19sep0518h100ml.pcr @18:46:46.31 Building PCR Slug from ASV with MM2 mix, PP4 primer @18:46:46.34 Priming with carrier @18:46:46.36 ATV.dial ARV @18:46:46.99 ARV.dial :carrier @18:46:47.36 AS.seek 0.5ml @18:47:12.93 AFV.select :carrierSolution @18:47:13.05 delay 2 seconds @18:47:15.07 AS.reconfigure ASconfig @18:47:15.14 AS.seek 1ml @18:47:44.22 delay 1 second @18:47:45.25 AFV.select :holdingCoil @18:47:45.35 delay 1 second @18:47:46.37 ATV.dial :waste @18:47:48.05 AS.reconfigure ASnorm @18:47:48.11 AS.seek :empty @18:48:38.87 AFV.select :carrierSolution @18:48:38.99 delay 2 seconds @18:48:41.01 AS.reconfigure ASconfig @18:48:41.07 AS.seek 0.5ml @18:49:10.18 delay 1 second @18:49:11.21 AFV.select :holdingCoil @18:49:11.31 Building Slug @18:49:11.32 ATV.dial :ARV @18:49:13.09 delay 2 seconds @18:49:15.11 ARV.dial :air1 @18:49:15.52 delay 2 seconds @18:49:17.60 AS.reconfigure ASslow2 @18:49:17.66 AS.seek 0.506ml @18:49:24.05 delay 2 seconds @18:49:26.08 ARV.dial :MM2 @18:49:27.59 delay 2 seconds @18:49:29.61 Adding MM2 @18:49:29.70 AS.seek 0.524ml @18:49:48.26 delay 25 seconds @18:50:13.29 ARV.dial :PP4 @18:50:13.69 Adding PP4 @18:50:13.70 delay 2 seconds @18:50:15.78 AS.seek 0.53ml @18:50:22.21 delay 10 seconds @18:50:32.25 ARV.dial :air3 @18:50:32.66 adding ARV air @18:50:32.67 delay 2 seconds @18:50:34.75 AS.seek 0.536ml @18:50:41.22 delay 5 seconds @18:50:46.25 ARV.dial :carrier @18:50:47.51 delay 7 seconds @18:50:54.60 AS.seek 0.594ml @18:51:53.15 delay 5 seconds @18:51:58.18 ATV.dial :ASV @18:51:58.58 delay 2 seconds @18:52:00.66 AS.seek 0.6ml @18:52:06.88 delay 4 seconds @18:52:10.91 ATV.dial ARV @18:52:11.44 delay 2 seconds @18:52:13.47 ARV.dial :carrier @18:52:13.48 delay 2 seconds @18:52:15.56 AS.seek 0.65ml @18:53:06.10 delay 4 seconds @18:53:10.13 ATV.dial PCR @18:53:11.27 ASV.dial :waste1 @18:53:11.29 ARV.dial :air1 @18:53:11.72 Positioning Slug by 0.324ml @18:53:11.81 AS.seek 0.326ml @18:58:38.02 delay 5 seconds @18:58:43.05 ATV.dial ARV @18:58:43.82 ASV.dial :PCRlock @18:58:44.23 delay 5 seconds @18:58:49.25 Running Ss Profile60C350 thermocycling @18:58:49.26 PCR.program Profile60C350 @18:58:49.42 Profile60C350 should complete in < 1:48:05 @18:58:49.43 PCR.run 2:20:06.25 @20:23:23.44 PCR.off @20:23:25.47 PCR for Ss completed -- closed /var/log/esp/Ss19sep0518h100ml.pcr @20:23:25.55 Cleaning PCR @20:23:25.56 AFV.select :carrierSolution @20:23:25.68 delay 2 seconds @20:23:27.70 AS.reconfigure ASconfig @20:23:27.76 AS.seek 0.5ml @20:23:38.05 delay 1 second @20:23:39.07 AFV.select :holdingCoil @20:23:39.18 ASV.dial :waste1 @20:23:39.56 ATV.dial :air3 @20:23:40.84 AS.reconfigure ASslow1 @20:23:40.91 AS.seek 0.51ml @20:23:43.18 delay 2 seconds @20:23:45.21 ATV.dial :bleach @20:23:45.83 AS.seek 0.81ml @20:24:46.47 delay 3 seconds @20:24:49.50 ATV.dial :air3 @20:24:50.06 delay 3 seconds @20:24:53.13 AS.seek 0.835ml @20:24:58.42 delay 3 seconds @20:25:01.45 ATV.dial :bleach @20:25:02.08 AS.seek 0.82ml @20:25:05.38 delay 3 seconds @20:25:08.41 ARV.dial :waste @20:25:09.02 delay 3 seconds @20:25:12.05 ATV.dial :ARV @20:25:12.95 AS.reconfigure ASconfig @20:25:13.01 AS.seek 0.47ml @20:25:33.42 AFV.select :carrierSolution @20:25:33.54 delay 2 seconds @20:25:35.56 AS.seek 1ml @20:26:06.30 delay 1 second @20:26:07.33 AFV.select :holdingCoil @20:26:07.43 delay 1 second @20:26:08.45 AS.reconfigure ASfast @20:26:08.51 AS.seek 0ml @20:26:35.86 AFV.select :carrierSolution @20:26:35.98 delay 2 seconds @20:26:38.00 AS.reconfigure ASconfig @20:26:38.06 AS.seek 1ml @20:27:35.86 delay 1 second @20:27:36.88 AFV.select :holdingCoil @20:27:36.99 delay 1 second @20:27:38.01 AS.reconfigure ASfast @20:27:38.07 AS.seek 0ml @20:28:05.41 AFV.select :carrierSolution @20:28:05.53 delay 2 seconds @20:28:07.55 AS.reconfigure ASconfig @20:28:07.61 AS.seek 1ml @20:29:05.38 delay 1 second @20:29:06.40 AFV.select :holdingCoil @20:29:06.51 delay 1 second @20:29:07.53 AS.reconfigure ASfast @20:29:07.59 AS.seek 0ml @20:29:34.92 ARV.dial between carrier and air1 @20:29:35.43 ATV.dial :air3 @20:29:36.70 AS.reconfigure ASslow1 @20:29:36.77 AS.seek 0.01ml @20:29:39.03 delay 2 seconds @20:29:41.06 ATV.dial :bleach @20:29:41.67 AS.seek 0.81ml @20:32:22.78 delay 3 seconds @20:32:25.81 ATV.dial :air3 @20:32:26.34 delay 3 seconds @20:32:29.42 AS.seek 0.835ml @20:32:34.86 delay 3 seconds @20:32:37.89 ATV.dial :bleach @20:32:38.50 AS.seek 0.82ml @20:32:41.81 delay 3 seconds @20:32:44.84 ATV.dial PCR @20:32:45.26 AS.reconfigure ASconfig @20:32:45.32 AS.seek :empty @20:33:32.77 PCR.program :bleachProfile @20:33:32.83 PCR program should complete in < 2:45 @20:33:32.84 PCR.run 10:00 @20:36:32.15 PCR.off @20:36:32.18 AFV.select :carrierSolution @20:36:32.30 delay 2 seconds @20:36:34.32 AS.seek 1ml @20:37:32.13 delay 1 second @20:37:33.15 AFV.select :holdingCoil @20:37:33.26 delay 1 second @20:37:34.28 AS.reconfigure ASfast @20:37:34.35 AS.seek 0ml @20:38:01.69 delay 3 seconds @20:38:04.71 AFV.select :carrierSolution @20:38:04.82 delay 2 seconds @20:38:06.84 AS.reconfigure ASconfig @20:38:06.90 AS.seek 1ml @20:39:04.69 delay 1 second @20:39:05.71 AFV.select :holdingCoil @20:39:05.82 delay 1 second @20:39:06.84 AS.reconfigure ASfast @20:39:06.90 AS.seek 0ml @20:39:34.25 delay 3 seconds @20:39:37.27 AFV.select :carrierSolution @20:39:37.38 delay 2 seconds @20:39:39.40 AS.reconfigure ASconfig @20:39:39.46 AS.seek 1ml @20:40:37.25 delay 1 second @20:40:38.27 AFV.select :holdingCoil @20:40:38.38 delay 1 second @20:40:39.40 AS.reconfigure ASfast @20:40:39.46 AS.seek 0ml @20:41:06.80 delay 3 seconds @20:41:09.82 AFV.select :carrierSolution @20:41:09.93 delay 2 seconds @20:41:11.95 AS.reconfigure ASconfig @20:41:12.01 AS.seek 1ml @20:42:09.77 delay 1 second @20:42:10.79 AFV.select :holdingCoil @20:42:10.90 delay 1 second @20:42:11.92 AS.reconfigure ASfast @20:42:11.98 AS.seek 0ml @20:42:39.33 delay 3 seconds @20:42:42.35 AFV.select :carrierSolution @20:42:42.46 delay 2 seconds @20:42:44.48 AS.reconfigure ASconfig @20:42:44.54 AS.seek 1ml @20:43:42.33 delay 1 second @20:43:43.35 AFV.select :holdingCoil @20:43:43.46 delay 1 second @20:43:44.48 AS.reconfigure ASnorm @20:43:44.54 AS.seek 0ml @20:44:35.27 Setting up PCR for Mm -- writing /var/log/esp/Mm19sep0520h100ml.pcr @20:44:35.29 Building PCR Slug from ASV with MM2 mix, PP3 primer @20:44:35.31 Priming with carrier @20:44:35.33 ATV.dial ARV @20:44:35.95 ARV.dial :carrier @20:44:36.32 AS.seek 0.5ml @20:45:01.92 AFV.select :carrierSolution @20:45:02.05 delay 2 seconds @20:45:04.07 AS.reconfigure ASconfig @20:45:04.14 AS.seek 1ml @20:45:33.16 delay 1 second @20:45:34.19 AFV.select :holdingCoil @20:45:34.29 delay 1 second @20:45:35.31 ATV.dial :waste @20:45:36.98 AS.reconfigure ASnorm @20:45:37.04 AS.seek :empty @20:46:27.78 AFV.select :carrierSolution @20:46:27.90 delay 2 seconds @20:46:29.92 AS.reconfigure ASconfig @20:46:29.99 AS.seek 0.5ml @20:46:59.13 delay 1 second @20:47:00.16 AFV.select :holdingCoil @20:47:00.26 Building Slug @20:47:00.27 ATV.dial :ARV @20:47:01.95 delay 2 seconds @20:47:03.97 ARV.dial :air1 @20:47:04.39 delay 2 seconds @20:47:06.47 AS.reconfigure ASslow2 @20:47:06.53 AS.seek 0.506ml @20:47:12.89 delay 2 seconds @20:47:14.92 ARV.dial :MM2 @20:47:16.41 delay 2 seconds @20:47:18.44 Adding MM2 @20:47:18.53 AS.seek 0.524ml @20:47:36.98 delay 25 seconds @20:48:02.01 ARV.dial :PP3 @20:48:02.42 Adding PP3 @20:48:02.43 delay 2 seconds @20:48:04.51 AS.seek 0.53ml @20:48:10.73 delay 10 seconds @20:48:20.77 ARV.dial :air2 @20:48:21.18 adding ARV air @20:48:21.19 delay 2 seconds @20:48:23.27 AS.seek 0.536ml @20:48:29.64 delay 5 seconds @20:48:34.67 ARV.dial :carrier @20:48:35.94 delay 7 seconds @20:48:43.03 AS.seek 0.594ml @20:49:41.47 delay 5 seconds @20:49:46.50 ATV.dial :ASV @20:49:46.91 delay 2 seconds @20:49:48.99 AS.seek 0.6ml @20:49:55.27 delay 4 seconds @20:49:59.30 ATV.dial ARV @20:50:00.46 delay 2 seconds @20:50:02.49 ARV.dial :carrier @20:50:02.50 delay 2 seconds @20:50:04.58 AS.seek 0.65ml @20:50:55.14 delay 4 seconds @20:50:59.17 ATV.dial PCR @20:50:59.79 ASV.dial :waste1 @20:50:59.80 ARV.dial :air1 @20:51:00.24 Positioning Slug by 0.324ml @20:51:00.33 AS.seek 0.326ml @20:56:26.36 delay 5 seconds @20:56:31.39 ATV.dial ARV @20:56:32.16 ASV.dial :PCRlock @20:56:32.59 delay 5 seconds @20:56:37.62 Running Mm Profile60C350 thermocycling @20:56:37.63 PCR.program Profile60C350 @20:56:37.79 Profile60C350 should complete in < 1:48:05 @20:56:37.80 PCR.run 2:20:06.25 @22:21:16.30 PCR.off @22:21:18.39 PCR for Mm completed -- closed /var/log/esp/Mm19sep0520h100ml.pcr @22:21:18.47 Cleaning PCR @22:21:18.48 AFV.select :carrierSolution @22:21:18.60 delay 2 seconds @22:21:20.62 AS.reconfigure ASconfig @22:21:20.68 AS.seek 0.5ml @22:21:30.98 delay 1 second @22:21:32.00 AFV.select :holdingCoil @22:21:32.11 ASV.dial :waste1 @22:21:32.51 ATV.dial :air3 @22:21:33.79 AS.reconfigure ASslow1 @22:21:33.86 AS.seek 0.51ml @22:21:36.15 delay 2 seconds @22:21:38.18 ATV.dial :bleach @22:21:38.79 AS.seek 0.81ml @22:22:39.40 delay 3 seconds @22:22:42.43 ATV.dial :air3 @22:22:42.95 delay 3 seconds @22:22:46.03 AS.seek 0.835ml @22:22:51.32 delay 3 seconds @22:22:54.35 ATV.dial :bleach @22:22:54.97 AS.seek 0.82ml @22:22:58.26 delay 3 seconds @22:23:01.29 ARV.dial :waste @22:23:01.89 delay 3 seconds @22:23:04.92 ATV.dial :ARV @22:23:05.82 AS.reconfigure ASconfig @22:23:05.88 AS.seek 0.47ml @22:23:26.29 AFV.select :carrierSolution @22:23:26.41 delay 2 seconds @22:23:28.43 AS.seek 1ml @22:23:59.20 delay 1 second @22:24:00.22 AFV.select :holdingCoil @22:24:00.33 delay 1 second @22:24:01.35 AS.reconfigure ASfast @22:24:01.41 AS.seek 0ml @22:24:28.76 AFV.select :carrierSolution @22:24:28.88 delay 2 seconds @22:24:30.90 AS.reconfigure ASconfig @22:24:30.96 AS.seek 1ml @22:25:28.75 delay 1 second @22:25:29.77 AFV.select :holdingCoil @22:25:29.88 delay 1 second @22:25:30.90 AS.reconfigure ASfast @22:25:30.96 AS.seek 0ml @22:25:58.42 AFV.select :carrierSolution @22:25:58.54 delay 2 seconds @22:26:00.56 AS.reconfigure ASconfig @22:26:00.62 AS.seek 1ml @22:26:58.40 delay 1 second @22:26:59.42 AFV.select :holdingCoil @22:26:59.53 delay 1 second @22:27:00.55 AS.reconfigure ASfast @22:27:00.61 AS.seek 0ml @22:27:27.98 ARV.dial between carrier and air1 @22:27:28.49 ATV.dial :air3 @22:27:29.76 AS.reconfigure ASslow1 @22:27:29.83 AS.seek 0.01ml @22:27:32.18 delay 2 seconds @22:27:34.21 ATV.dial :bleach @22:27:34.82 AS.seek 0.81ml @22:30:16.01 delay 3 seconds @22:30:19.04 ATV.dial :air3 @22:30:19.57 delay 3 seconds @22:30:22.65 AS.seek 0.835ml @22:30:27.95 delay 3 seconds @22:30:30.98 ATV.dial :bleach @22:30:31.59 AS.seek 0.82ml @22:30:34.90 delay 3 seconds @22:30:37.93 ATV.dial PCR @22:30:38.35 AS.reconfigure ASconfig @22:30:38.41 AS.seek :empty @22:31:26.36 PCR.program :bleachProfile @22:31:26.42 PCR program should complete in < 2:45 @22:31:26.43 PCR.run 10:00 @22:34:28.28 PCR.off @22:34:28.31 AFV.select :carrierSolution @22:34:28.42 delay 2 seconds @22:34:30.44 AS.seek 1ml @22:35:28.26 delay 1 second @22:35:29.29 AFV.select :holdingCoil @22:35:29.39 delay 1 second @22:35:30.41 AS.reconfigure ASfast @22:35:30.47 AS.seek 0ml @22:35:57.82 delay 3 seconds @22:36:00.85 AFV.select :carrierSolution @22:36:00.96 delay 2 seconds @22:36:02.98 AS.reconfigure ASconfig @22:36:03.05 AS.seek 1ml @22:37:00.84 delay 1 second @22:37:01.87 AFV.select :holdingCoil @22:37:01.98 delay 1 second @22:37:03.00 AS.reconfigure ASfast @22:37:03.06 AS.seek 0ml @22:37:30.42 delay 3 seconds @22:37:33.45 AFV.select :carrierSolution @22:37:33.55 delay 2 seconds @22:37:35.57 AS.reconfigure ASconfig @22:37:35.64 AS.seek 1ml @22:38:33.43 delay 1 second @22:38:34.46 AFV.select :holdingCoil @22:38:34.56 delay 1 second @22:38:35.58 AS.reconfigure ASfast @22:38:35.64 AS.seek 0ml @22:39:03.00 delay 3 seconds @22:39:06.02 AFV.select :carrierSolution @22:39:06.13 delay 2 seconds @22:39:08.15 AS.reconfigure ASconfig @22:39:08.22 AS.seek 1ml @22:40:06.00 delay 1 second @22:40:07.02 AFV.select :holdingCoil @22:40:07.13 delay 1 second @22:40:08.15 AS.reconfigure ASfast @22:40:08.21 AS.seek 0ml @22:40:35.56 delay 3 seconds @22:40:38.58 AFV.select :carrierSolution @22:40:38.69 delay 2 seconds @22:40:40.71 AS.reconfigure ASconfig @22:40:40.77 AS.seek 1ml @22:41:38.53 delay 1 second @22:41:39.55 AFV.select :holdingCoil @22:41:39.66 delay 1 second @22:41:40.68 AS.reconfigure ASnorm @22:41:40.74 AS.seek 0ml @22:42:31.45 Setting up PCR for IPc -- writing /var/log/esp/IPc19sep0522h100ml.pcr @22:42:31.47 Building PCR Slug from ASV with MM1 mix, PP1 primer @22:42:31.49 Priming with carrier @22:42:31.51 ATV.dial ARV @22:42:32.14 ARV.dial :carrier @22:42:32.50 AS.seek 0.5ml @22:42:58.06 AFV.select :carrierSolution @22:42:58.19 delay 2 seconds @22:43:00.21 AS.reconfigure ASconfig @22:43:00.28 AS.seek 1ml @22:43:29.29 delay 1 second @22:43:30.32 AFV.select :holdingCoil @22:43:30.42 delay 1 second @22:43:31.44 ATV.dial :waste @22:43:33.13 AS.reconfigure ASnorm @22:43:33.19 AS.seek :empty @22:44:23.93 AFV.select :carrierSolution @22:44:24.05 delay 2 seconds @22:44:26.07 AS.reconfigure ASconfig @22:44:26.14 AS.seek 0.5ml @22:44:55.21 delay 1 second @22:44:56.23 AFV.select :holdingCoil @22:44:56.34 Building Slug @22:44:56.35 ATV.dial :ARV @22:44:58.02 delay 2 seconds @22:45:00.04 ARV.dial :air1 @22:45:00.44 delay 2 seconds @22:45:02.52 AS.reconfigure ASslow2 @22:45:02.58 AS.seek 0.506ml @22:45:09.04 delay 2 seconds @22:45:11.07 ARV.dial :MM1 @22:45:11.67 delay 2 seconds @22:45:13.70 Adding MM1 @22:45:13.78 AS.seek 0.524ml @22:45:32.17 delay 25 seconds @22:45:57.20 ARV.dial :PP1 @22:45:57.61 Adding PP1 @22:45:57.62 delay 2 seconds @22:45:59.70 AS.seek 0.53ml @22:46:06.39 delay 10 seconds @22:46:16.43 ARV.dial :air1 @22:46:16.82 adding ARV air @22:46:16.83 delay 2 seconds @22:46:18.91 AS.seek 0.536ml @22:46:25.42 delay 5 seconds @22:46:30.45 ARV.dial :carrier @22:46:30.84 delay 7 seconds @22:46:38.43 AS.seek 0.594ml @22:47:36.92 delay 5 seconds @22:47:41.95 ATV.dial :ASV @22:47:42.36 delay 2 seconds @22:47:44.43 AS.seek 0.6ml @22:47:50.68 delay 4 seconds @22:47:54.71 ATV.dial ARV @22:47:55.36 delay 2 seconds @22:47:57.38 ARV.dial :carrier @22:47:57.39 delay 2 seconds @22:47:59.47 AS.seek 0.65ml @22:48:49.87 delay 4 seconds @22:48:53.90 ATV.dial PCR @22:48:54.51 ASV.dial :waste1 @22:48:54.53 ARV.dial :air1 @22:48:55.05 Positioning Slug by 0.324ml @22:48:55.14 AS.seek 0.326ml @22:54:21.18 delay 5 seconds @22:54:26.21 ATV.dial ARV @22:54:26.98 ASV.dial :PCRlock @22:54:27.40 delay 5 seconds @22:54:32.42 Running IPc ProfileIPc thermocycling @22:54:32.43 PCR.program ProfileIPc @22:54:32.59 ProfileIPc should complete in < 1:50:49 @22:54:32.60 PCR.run 2:23:31.25 @00:21:09.08CEST06-Sep-19 PCR.off @00:21:11.59 PCR for IPc completed -- closed /var/log/esp/IPc19sep0522h100ml.pcr @00:21:11.61 Full MFB Decontamination @00:21:11.63 Decon extraction column and decon AIV @00:21:11.67 SPE.hold 2:46:40,55C @00:21:11.72 ASV.dial :SPEdelivery @00:21:12.39 delay 20 seconds @00:21:32.41 AFV.select :carrierSolution @00:21:32.52 delay 2 seconds @00:21:34.54 AS.reconfigure ASconfig @00:21:34.60 AS.seek 0.5ml @00:21:44.91 delay 1 second @00:21:45.93 AFV.select :holdingCoil @00:21:46.04 ATV.dial :air1 @00:21:48.02 AS.reconfigure ASslow1 @00:21:48.09 AS.seek 0.52ml @00:21:52.38 ATV.dial :ASV @00:21:54.20 ASV.dial :SPErecovery @00:21:54.89 AS.seek 0.62ml @00:22:15.27 ATV.dial :waste @00:22:17.47 AS.reconfigure ASnorm @00:22:17.53 AS.seek :empty @00:22:49.16 AFV.select :holdingCoil @00:22:49.18 ATV.dial :waste @00:22:49.21 AS.reconfigure ASconfig @00:22:49.27 AS.seek 0ml @00:22:49.59 AFV.select :carrierSolution @00:22:49.71 delay 2 seconds @00:22:51.73 AS.seek 0.5ml @00:23:20.77 delay 1 second @00:23:21.79 AFV.select :holdingCoil @00:23:21.90 ATV.dial :air3 @00:23:22.63 AS.reconfigure ASslow1 @00:23:22.70 AS.seek 0.52ml @00:23:27.04 ATV.dial :air3 @00:23:27.14 AS.seek 0.53ml @00:23:29.88 delay 2 seconds @00:23:31.91 ATV.dial :bleach @00:23:32.40 AS.seek 0.71ml @00:24:08.98 delay 3 seconds @00:24:12.01 ATV.dial :air3 @00:24:12.61 delay 3 seconds @00:24:15.68 AS.seek 0.735ml @00:24:21.01 delay 3 seconds @00:24:24.04 ATV.dial :bleach @00:24:24.69 AS.seek 0.72ml @00:24:28.07 delay 3 seconds @00:24:31.10 ATV.dial ASV @00:24:32.20 ASV.dial :SPErecovery @00:24:32.30 AS.seek 0.365ml @00:25:43.96 ATV.dial :waste @00:25:45.77 AS.reconfigure ASnorm @00:25:45.83 AS.seek :empty @00:26:04.62 ATV.dial :air3 @00:26:05.32 AS.reconfigure ASslow1 @00:26:05.39 AS.seek 0.009ml @00:26:07.71 delay 2 seconds @00:26:09.74 ATV.dial :bleach @00:26:10.23 AS.seek 0.579ml @00:28:05.12 delay 3 seconds @00:28:08.15 ATV.dial :air3 @00:28:08.71 delay 3 seconds @00:28:11.79 AS.seek 0.604ml @00:28:17.10 delay 3 seconds @00:28:20.13 ATV.dial :bleach @00:28:20.75 AS.seek 0.589ml @00:28:24.05 delay 3 seconds @00:28:27.08 ATV.dial :ASV @00:28:28.13 ASV.dial :wasteR @00:28:28.64 AS.seek 0ml @00:30:27.38 delay 1:00 @00:31:27.42 ATV.dial ASV @00:31:27.43 AFV.select :carrierSolution @00:31:27.55 delay 2 seconds @00:31:29.57 AS.reconfigure ASconfig @00:31:29.63 AS.seek 1ml @00:32:27.43 delay 1 second @00:32:28.45 AFV.select :holdingCoil @00:32:28.56 delay 1 second @00:32:29.58 ASV.dial :wasteR @00:32:29.59 AS.reconfigure ASslow1 @00:32:29.67 AS.seek 0ml @00:35:51.04 delay 10 seconds @00:36:01.08 ATV.dial :air3 @00:36:02.61 AS.seek 0.01ml @00:36:04.99 delay 2 seconds @00:36:07.02 ATV.dial :bleach @00:36:07.63 AS.seek 0.58ml @00:38:02.54 delay 3 seconds @00:38:05.57 ATV.dial :air3 @00:38:06.13 delay 3 seconds @00:38:09.21 AS.seek 0.605ml @00:38:14.52 delay 3 seconds @00:38:17.55 ATV.dial :bleach @00:38:18.16 AS.seek 0.59ml @00:38:21.44 delay 3 seconds @00:38:24.47 ATV.dial ASV @00:38:25.63 ASV.dial :SPEdelivery @00:38:26.03 AS.seek 0ml @00:40:24.93 delay 1:00 @00:41:24.97 AFV.select :carrierSolution @00:41:25.07 delay 2 seconds @00:41:27.09 AS.reconfigure ASconfig @00:41:27.15 AS.seek 1ml @00:42:24.96 delay 1 second @00:42:25.98 AFV.select :holdingCoil @00:42:26.09 delay 1 second @00:42:27.11 ATV.dial ASV @00:42:27.12 ASV.dial :SPEdelivery @00:42:27.15 AS.reconfigure ASslow1 @00:42:27.22 AS.seek 0ml @00:45:48.62 delay 10 seconds @00:45:58.66 ATV.dial :air2 @00:45:59.52 AS.reconfigure ASslow0 @00:45:59.58 AS.seek 0.8ml @00:47:20.38 delay 3 seconds @00:47:23.41 ATV.dial :ASV @00:47:24.41 ASV.dial :SPEdelivery @00:47:24.44 AS.reconfigure ASslow1 @00:47:24.51 AS.seek 0.03ml @00:49:59.66 delay 20 seconds @00:50:19.70 ATV.dial :waste,via: :air3 @00:50:21.44 AS.reconfigure ASconfig @00:50:21.49 AS.seek :empty @00:50:24.09 SPE.coast @00:50:24.18 Removing Bleach from Mixing Coil @00:50:24.19 AFV.select :carrierSolution @00:50:24.30 delay 2 seconds @00:50:26.32 AS.seek 0.5ml @00:50:55.43 delay 1 second @00:50:56.45 AFV.select :holdingCoil @00:50:56.56 ATV.dial :ASV @00:50:58.27 ASV.dial :SPErecovery @00:50:58.93 AS.reconfigure ASslow1 @00:50:59.00 AS.seek 0.73ml @00:51:45.54 ASV.dial :wasteD @00:51:46.14 AS.reconfigure ASnorm @00:51:46.20 AS.seek 0ml @00:52:23.34 AFV.select :carrierSolution @00:52:23.46 delay 2 seconds @00:52:25.48 AS.reconfigure ASconfig @00:52:25.54 AS.seek 0.5ml @00:52:54.60 delay 1 second @00:52:55.63 AFV.select :holdingCoil @00:52:55.73 ATV.dial ASV @00:52:55.74 ASV.dial :SPErecovery @00:52:56.40 AS.reconfigure ASslow0 @00:52:56.47 AS.seek 0.3ml @00:53:16.88 ATV.dial :air2 @00:53:17.94 AS.reconfigure ASslow1 @00:53:18.01 AS.seek 0.32ml @00:53:22.38 ATV.dial ASV @00:53:23.44 AS.reconfigure ASslow0 @00:53:23.50 AS.seek 0.57ml @00:53:48.94 ASV.dial :wasteD @00:53:49.64 AS.reconfigure ASnorm @00:53:49.70 AS.seek 0ml @00:54:18.79 AFV.select :carrierSolution @00:54:18.91 delay 2 seconds @00:54:20.93 AS.reconfigure ASconfig @00:54:20.99 AS.seek 0.5ml @00:54:50.04 delay 1 second @00:54:51.06 AFV.select :holdingCoil @00:54:51.17 ATV.dial ASV @00:54:51.18 ASV.dial :SPErecovery @00:54:51.82 AS.reconfigure ASslow0 @00:54:51.88 AS.seek 0.3ml @00:55:12.30 ATV.dial :air2 @00:55:13.36 AS.reconfigure ASslow1 @00:55:13.43 AS.seek 0.32ml @00:55:17.75 ATV.dial ASV @00:55:18.80 AS.reconfigure ASslow0 @00:55:18.86 AS.seek 0.57ml @00:55:44.33 ASV.dial :wasteD @00:55:44.94 AS.reconfigure ASnorm @00:55:45.00 AS.seek 0ml @00:56:14.07 ATV.dial :air2 @00:56:15.08 AS.reconfigure ASslow0 @00:56:15.14 AS.seek 0.3ml @00:56:45.69 ATV.dial :ASV @00:56:46.69 ASV.dial :wasteD @00:56:46.71 AS.reconfigure ASconfig @00:56:46.78 AS.seek 0.05ml @00:57:01.46 ATV.dial :waste @00:57:03.36 AS.seek 0ml @00:57:06.58 ASV.dial :SPEdelivery @00:57:07.60 deconLysateINT @00:57:07.61 ATV.dial :air3 @00:57:08.30 AS.reconfigure ASslow1 @00:57:08.36 AS.seek 0.01ml @00:57:10.76 delay 2 seconds @00:57:12.79 ATV.dial :bleach @00:57:13.27 AS.seek 0.96ml @01:00:24.68 delay 3 seconds @01:00:27.71 ATV.dial :air3 @01:00:28.26 delay 3 seconds @01:00:31.34 AS.seek 0.985ml @01:00:36.69 delay 3 seconds @01:00:39.72 ATV.dial :bleach @01:00:40.34 AS.seek 0.97ml @01:00:43.65 delay 3 seconds @01:00:46.68 ATV.dial AIV @01:00:47.94 AIV.dial :coilToMFB @01:00:47.96 AWV.select :waste @01:00:47.97 AFV.select :carrierSolution @01:00:48.11 delay 2 seconds @01:00:50.13 AS.reconfigure ASconfig @01:00:50.19 AS.seek 1ml @01:00:52.73 delay 1 second @01:00:53.76 AFV.select :holdingCoil @01:00:53.87 delay 1 second @01:00:54.89 AS.reconfigure ASslow0 @01:00:54.95 AS.seek :empty @01:02:35.90 AFV.select :carrierSolution @01:02:36.03 delay 2 seconds @01:02:38.05 AS.reconfigure ASconfig @01:02:38.12 AS.seek 1ml @01:03:35.93 delay 1 second @01:03:36.96 AFV.select :holdingCoil @01:03:37.06 delay 1 second @01:03:38.08 AS.reconfigure ASslow0 @01:03:38.14 AS.seek :empty @01:05:19.10 AFV.select :carrierSolution @01:05:19.23 delay 2 seconds @01:05:21.25 AS.reconfigure ASconfig @01:05:21.32 AS.seek 1ml @01:06:19.13 delay 1 second @01:06:20.16 AFV.select :holdingCoil @01:06:20.26 delay 1 second @01:06:21.28 AS.reconfigure ASslow0 @01:06:21.33 AS.seek :empty @01:08:02.29 ATV.dial :air2 @01:08:03.00 AS.reconfigure ASslow1 @01:08:03.06 AS.seek 0.009ml @01:08:05.39 AS.reconfigure ASslow0 @01:08:05.45 AS.seek 1ml @01:09:45.35 ATV.dial AIV @01:09:46.16 AS.seek 0.02ml @01:11:25.05 ATV.dial :waste @01:11:26.75 AS.seek 0ml @01:11:29.05 AWV.select :air @01:11:29.21 Cleaning PCR @01:11:29.22 AFV.select :carrierSolution @01:11:29.35 delay 2 seconds @01:11:31.37 AS.reconfigure ASconfig @01:11:31.43 AS.seek 0.5ml @01:12:00.51 delay 1 second @01:12:01.53 AFV.select :holdingCoil @01:12:01.64 ASV.dial :waste1 @01:12:02.02 ATV.dial :air3 @01:12:02.71 AS.reconfigure ASslow1 @01:12:02.78 AS.seek 0.51ml @01:12:05.07 delay 2 seconds @01:12:07.10 ATV.dial :bleach @01:12:07.55 AS.seek 0.81ml @01:13:08.10 delay 3 seconds @01:13:11.13 ATV.dial :air3 @01:13:11.68 delay 3 seconds @01:13:14.76 AS.seek 0.835ml @01:13:20.13 delay 3 seconds @01:13:23.16 ATV.dial :bleach @01:13:23.77 AS.seek 0.82ml @01:13:27.02 delay 3 seconds @01:13:30.05 ARV.dial :waste @01:13:30.65 delay 3 seconds @01:13:33.68 ATV.dial :ARV @01:13:34.57 AS.reconfigure ASconfig @01:13:34.63 AS.seek 0.47ml @01:13:55.05 AFV.select :carrierSolution @01:13:55.17 delay 2 seconds @01:13:57.19 AS.seek 1ml @01:14:27.93 delay 1 second @01:14:28.95 AFV.select :holdingCoil @01:14:29.06 delay 1 second @01:14:30.08 AS.reconfigure ASfast @01:14:30.14 AS.seek 0ml @01:14:57.49 AFV.select :carrierSolution @01:14:57.61 delay 2 seconds @01:14:59.63 AS.reconfigure ASconfig @01:14:59.69 AS.seek 1ml @01:15:57.43 delay 1 second @01:15:58.46 AFV.select :holdingCoil @01:15:58.56 delay 1 second @01:15:59.58 AS.reconfigure ASfast @01:15:59.64 AS.seek 0ml @01:16:26.99 AFV.select :carrierSolution @01:16:27.11 delay 2 seconds @01:16:29.13 AS.reconfigure ASconfig @01:16:29.19 AS.seek 1ml @01:17:26.93 delay 1 second @01:17:27.96 AFV.select :holdingCoil @01:17:28.06 delay 1 second @01:17:29.08 AS.reconfigure ASfast @01:17:29.14 AS.seek 0ml @01:17:56.51 ARV.dial between carrier and air1 @01:17:57.03 ATV.dial :air3 @01:17:58.32 AS.reconfigure ASslow1 @01:17:58.39 AS.seek 0.01ml @01:18:00.72 delay 2 seconds @01:18:02.75 ATV.dial :bleach @01:18:03.38 AS.seek 0.81ml @01:20:44.54 delay 3 seconds @01:20:47.57 ATV.dial :air3 @01:20:48.12 delay 3 seconds @01:20:51.20 AS.seek 0.835ml @01:20:56.53 delay 3 seconds @01:20:59.56 ATV.dial :bleach @01:21:00.70 AS.seek 0.82ml @01:21:03.99 delay 3 seconds @01:21:07.02 ATV.dial PCR @01:21:07.44 AS.reconfigure ASconfig @01:21:07.50 AS.seek :empty @01:21:54.96 PCR.program :bleachProfile @01:21:55.02 PCR program should complete in < 2:45 @01:21:55.04 PCR.run 10:00 @01:25:02.92 PCR.off @01:25:02.95 AFV.select :carrierSolution @01:25:03.07 delay 2 seconds @01:25:05.09 AS.seek 1ml @01:26:02.93 delay 1 second @01:26:03.96 AFV.select :holdingCoil @01:26:04.06 delay 1 second @01:26:05.08 AS.reconfigure ASfast @01:26:05.14 AS.seek 0ml @01:26:32.49 delay 3 seconds @01:26:35.52 AFV.select :carrierSolution @01:26:35.62 delay 2 seconds @01:26:37.64 AS.reconfigure ASconfig @01:26:37.71 AS.seek 1ml @01:27:35.51 delay 1 second @01:27:36.54 AFV.select :holdingCoil @01:27:36.64 delay 1 second @01:27:37.66 AS.reconfigure ASfast @01:27:37.72 AS.seek 0ml @01:28:05.05 delay 3 seconds @01:28:08.08 AFV.select :carrierSolution @01:28:08.18 delay 2 seconds @01:28:10.20 AS.reconfigure ASconfig @01:28:10.27 AS.seek 1ml @01:29:08.07 delay 1 second @01:29:09.10 AFV.select :holdingCoil @01:29:09.20 delay 1 second @01:29:10.22 AS.reconfigure ASfast @01:29:10.28 AS.seek 0ml @01:29:37.65 delay 3 seconds @01:29:40.68 AFV.select :carrierSolution @01:29:40.78 delay 2 seconds @01:29:42.80 AS.reconfigure ASconfig @01:29:42.86 AS.seek 1ml @01:30:40.60 delay 1 second @01:30:41.63 AFV.select :holdingCoil @01:30:41.74 delay 1 second @01:30:42.76 AS.reconfigure ASfast @01:30:42.82 AS.seek 0ml @01:31:10.19 delay 3 seconds @01:31:13.22 AFV.select :carrierSolution @01:31:13.33 delay 2 seconds @01:31:15.35 AS.reconfigure ASconfig @01:31:15.42 AS.seek 1ml @01:32:13.16 delay 1 second @01:32:14.18 AFV.select :holdingCoil @01:32:14.29 delay 1 second @01:32:15.31 AS.reconfigure ASnorm @01:32:15.37 AS.seek 0ml @01:33:06.09 AFV.select :carrierSolution @01:33:06.20 delay 2 seconds @01:33:08.22 AS.reconfigure ASconfig @01:33:08.28 AS.seek 1ml @01:34:06.02 delay 1 second @01:34:07.04 AFV.select :holdingCoil @01:34:07.15 delay 1 second @01:34:08.17 ATV.dial :waste @01:34:09.41 AS.reconfigure ASnorm @01:34:09.47 AS.seek 0.5ml @01:34:35.02 ATV.dial :waste2 @01:34:36.33 AS.seek 0ml @01:35:01.88
Asleep after completing 190905totalPostcanqc assays @01:35:01.89 GoodNews.email "{1 => 6, 2 => 16}\nCan@01:28:10, 21.8C, 78% humidity, 14.9psia, 12.051V, 0.443A, 0.443A avg, 5.34W",Subject: "Asleep after completing 190905totalPostcanqc assays" @01:35:01.92
Mission runtime was 10:36:52.729932 @01:35:07.99 Session Ended @07:04:44.68CEST10-Sep-19
>RESET< ESP2 v3.30 I2C Gateway v3.06 for core bus @07:04:46.76 Copyright 2018 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @07:04:47.07 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @07:04:52.75 Polling Can every 10:00 @07:04:52.79 Cleanly exited at 13:19:00CEST06-Sep-19 @07:04:52.83 Loading /home/esp/esp2/mission/TotalTRIAL.rb mission @07:04:52.90 Preparing to sleep until +00:01:00 @07:04:52.92 GoodNews.email "Starting TotalTRIAL w/pucks in tube 2\n{1 => 6, 2 => 16}\nCan@07:04:52, 15.4C, 81% humidity, 14.5psia, 15.633V, 0.134A, 0.134A avg, 2.09W",Subject: "TotalTRIAL begun" @07:04:53.01 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @07:04:53.05 Retry #1 of 10 after 10:00 @07:04:53.07 Shutdown @07:04:53.09
Asleep til +00:01:00 @07:04:53.11 Continuing at 07:05 @07:05:03.69 Session Begun @07:05:52.87
Powering core bus @07:05:57.95 Gate.power :raw,:ON @07:05:59.03 SPE.coast @07:05:59.12 SPE.configure SPEconfig @07:05:59.24 AS.configure ASconfig @07:05:59.36 SS.configure SSconfig @07:05:59.48 CC.configure CCconfig @07:05:59.60 CS.configure CSconfig @07:05:59.72 PC.configure PCconfig @07:05:59.84 PS.configure PSconfig @07:05:59.99 Forearm.configure LooseForearm @07:06:00.10 Elbow.configure LooseElbow @07:06:00.24 SC.configure SCloose @07:06:00.36 SE.configure SEconfig @07:06:00.43 CH.coast @07:06:00.50 CH.configure CHconfig @07:06:00.56 PH.coast @07:06:00.64 PH.configure PHconfig @07:06:00.70 PCR.off @07:06:00.74 PCR.pidtemp= 50,0.1,0,300,25,25 @07:06:00.78 PCR.caltemp= 2.8814,0.0372,0 @07:06:00.83 All dwarf controllers running firmware version 3.75 @07:06:00.96 Valve.reset @07:06:01.15 PCR.off @07:06:01.19 PCR.pidtemp= 50,0.1,0,300,25,25 @07:06:01.24 PCR.caltemp= 2.8814,0.0372,0 @07:06:01.42 Intake.select :close @07:06:01.46 Exhaust.select :close @07:06:01.48 CTV.dial :air @07:06:01.61 PSV.dial :air @07:06:01.70 CSV.dial :waste1 @07:06:01.86 SSV.dial :spit,avoiding: :exhaust @07:06:01.89 PS.reconfigure PSconfig @07:06:01.94
Forearm.reconfigure LooseForearm @07:06:02.10 PS.home.seek 0ml @07:06:02.12
Forearm.home.seek :retract @07:06:02.33 CS.reconfigure CSfast @07:06:02.36 SS.reconfigure SSconfig @07:06:02.50 CS.home.seek 0ml @07:06:02.54 SS.home.seek 0ml @07:06:02.93 Hand.close @07:06:02.96
Elbow.jog 1500 @07:06:03.50 Elbow.reconfigure LooseElbow @07:06:03.56 Elbow.home.seek :clear @07:06:08.27 SE.reconfigure SEconfig @07:06:08.34 SE.home.seek :down @07:06:09.02 SC.reconfigure SCsloppy @07:06:09.09 SC.home.seek :initial @07:06:11.11 PS.coast @07:06:13.01
Forearm.seek :retract @07:06:13.12 Elbow.seek :hammer @07:06:14.41 Forearm.reconfigure TightForearm @07:06:14.48 Forearm.seek 5 + 350 counts @07:06:15.61 Forearm.seek :retract @07:06:16.85 PC.reconfigure PCconfig @07:06:16.87
CC.reconfigure CCconfig @07:06:16.99 PC.home.seek :open @07:06:17.01
CC.home.seek :open @07:06:20.44 Phase [wcrPhase2] @07:06:21.06 Completed [wcrPhase2] begun 07:06:20 @07:06:21.12 Asleep after completing TotalTRIAL assays @07:06:21.14 Mission runtime was 1:28.267703 @07:06:38.05 -> exit @07:06:38.06 Session Ended @07:06:41.20
Sending Email Log::Locked in trapHandler -- Another process is already writing to /var/log/esp/real.log @07:07:00.22 Session Begun @07:07:04.43 -> exit @07:07:04.45 Session Ended @07:07:27.40 Session Begun @07:09:28.69 -> exit @07:09:28.71 Session Ended @07:09:32.72 Session Begun @07:09:41.22 Email::Error in MAIN -- flush failed! @07:09:41.29 Session Ended @07:12:03.83CEST10-Sep-19
ESP2 v3.30 I2C Gateway v3.06 for core bus @07:12:06.23 Copyright 2018 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @07:12:06.53 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @07:12:12.35 Polling Can every 10:00 @07:12:14.17 SPE.coast @07:12:14.24 SPE.configure SPEconfig @07:12:14.33 AS.configure ASconfig @07:12:14.43 SS.reconfigure SSconfig @07:12:14.48 SS.hold @07:12:14.53 SS.configure SSconfig @07:12:14.62 CC.reconfigure CCconfig @07:12:14.67 CC.hold @07:12:14.76 CC.configure CCconfig @07:12:14.86 CS.reconfigure CSconfig @07:12:14.90 CS.hold @07:12:14.97 CS.configure CSconfig @07:12:15.07 PC.reconfigure PCconfig @07:12:15.11 PC.hold @07:12:15.55 PC.configure PCconfig @07:12:15.65 PS.configure PSconfig @07:12:15.77 Forearm.reconfigure LooseForearm @07:12:15.81 Forearm.hold @07:12:15.88 Forearm.configure LooseForearm @07:12:15.98 Elbow.reconfigure LooseElbow @07:12:16.02 Elbow.hold @07:12:16.08 Elbow.configure LooseElbow @07:12:16.19 SC.reconfigure SCloose @07:12:16.24 SC.hold @07:12:16.30 SC.configure SCloose @07:12:16.39 SE.reconfigure SEconfig @07:12:16.43 SE.hold @07:12:16.50 SE.configure SEconfig @07:12:16.55 CH.coast @07:12:16.60 CH.configure CHconfig @07:12:16.63 PH.coast @07:12:16.69 PH.configure PHconfig @07:12:16.73 PCR.off @07:12:16.76 PCR.pidtemp= 50,0.1,0,300,25,25 @07:12:16.78 PCR.caltemp= 2.8814,0.0372,0 @07:12:16.81 All dwarf controllers running firmware version 3.75 @07:12:16.95 Cleanly exited at 07:09:41CEST10-Sep-19 @07:12:16.99 Loading /home/esp/esp2/mission/TotalTRIAL.rb mission @07:12:17.05 Preparing to sleep until +00:01:00 @07:12:17.07 GoodNews.email "Starting TotalTRIAL w/pucks in tube 2\n{1 => 6, 2 => 16}\nCan@07:12:12, 15.5C, 81% humidity, 14.5psia, 15.441V, 0.601A, 0.601A avg, 9.27W",Subject: "TotalTRIAL begun" @07:12:17.18 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @07:12:17.20 SS.coast @07:12:17.21
Asleep til +00:01:00 @07:12:17.23 Retry #1 of 10 after 10:00 @07:12:17.27
Continuing at 07:13 @07:12:17.30 CC.coast @07:12:17.38 CS.coast @07:12:17.43 PC.coast @07:12:17.47 Forearm.coast @07:12:17.50 Elbow.coast @07:12:17.54 SC.coast @07:12:17.57 SE.coast @07:12:17.96 Gate.power :core,:OFF @07:12:17.98 Gate.power :raw,:OFF @07:12:17.99 Shutdown @07:12:19.75 Session Begun @07:12:44.75 -> exit @07:12:44.77 Session Ended @07:13:09.93 Session Begun @07:13:17.03
Powering core bus @07:13:22.15 Gate.power :raw,:ON @07:13:22.86 SPE.coast @07:13:22.94 SPE.configure SPEconfig @07:13:23.06 AS.configure ASconfig @07:13:23.18 SS.configure SSconfig @07:13:23.30 CC.configure CCconfig @07:13:23.43 CS.configure CSconfig @07:13:23.55 PC.configure PCconfig @07:13:23.66 PS.configure PSconfig @07:13:23.81 Forearm.configure LooseForearm @07:13:23.92 Elbow.configure LooseElbow @07:13:24.07 SC.configure SCloose @07:13:24.18 SE.configure SEconfig @07:13:24.25 CH.coast @07:13:24.32 CH.configure CHconfig @07:13:24.38 PH.coast @07:13:24.46 PH.configure PHconfig @07:13:24.51 PCR.off @07:13:24.56 PCR.pidtemp= 50,0.1,0,300,25,25 @07:13:24.61 PCR.caltemp= 2.8814,0.0372,0 @07:13:24.65 All dwarf controllers running firmware version 3.75 @07:13:24.78 Valve.reset @07:13:24.97 PCR.off @07:13:25.01 PCR.pidtemp= 50,0.1,0,300,25,25 @07:13:25.06 PCR.caltemp= 2.8814,0.0372,0 @07:13:25.25 Intake.select :close @07:13:25.30 Exhaust.select :close @07:13:25.32 CTV.dial :air @07:13:25.43 PSV.dial :air @07:13:25.48 CSV.dial :waste1 @07:13:25.68 SSV.dial :spit,avoiding: :exhaust @07:13:25.72 PS.reconfigure PSconfig @07:13:25.75
Forearm.reconfigure LooseForearm @07:13:25.92 PS.home.seek 0ml @07:13:25.94
Forearm.home.seek :retract @07:13:26.16 CS.reconfigure CSfast @07:13:26.19 SS.reconfigure SSconfig @07:13:26.34 CS.home.seek 0ml @07:13:26.38 SS.home.seek 0ml @07:13:26.76 Hand.close @07:13:26.79
Elbow.jog 1500 @07:13:27.35 Elbow.reconfigure LooseElbow @07:13:27.42 Elbow.home.seek :clear @07:13:32.05 SE.reconfigure SEconfig @07:13:32.12 SE.home.seek :down @07:13:32.89 SC.reconfigure SCsloppy @07:13:32.96 SC.home.seek :initial @07:13:34.90 PS.coast @07:13:36.88
Forearm.seek :retract @07:13:36.98 Elbow.seek :hammer @07:13:38.30 Forearm.reconfigure TightForearm @07:13:38.37 Forearm.seek 5 + 350 counts @07:13:39.47 Forearm.seek :retract @07:13:40.71 PC.reconfigure PCconfig @07:13:40.73
CC.reconfigure CCconfig @07:13:40.82 PC.home.seek :open @07:13:40.91
CC.home.seek :open @07:13:44.32 Phase [wcrPhase2] @07:13:44.95 Completed [wcrPhase2] begun 07:13:44 @07:13:45.02 Asleep after completing TotalTRIAL assays @07:13:45.03 Mission runtime was 1:28.015123 @07:13:53.89 -> exit @07:13:53.90 Session Ended @07:14:05.09
Sending Email @07:19:28.49CEST10-Sep-19
>RESET< ESP2 v3.30 I2C Gateway v3.06 for core bus @07:19:30.48 Copyright 2018 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @07:19:30.79 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @07:19:36.89 Polling Can every 10:00 @07:19:36.94 Cleanly exited at 07:12:16CEST10-Sep-19 @07:19:36.98 Loading /home/esp/esp2/mission/TotaldeploymentFINAL.rb mission @07:19:37.06 Preparing to sleep until 20:12 10-september-19 @07:19:37.09 GoodNews.email "Starting TotaldeploymentFINAL w/pucks in tube 2\n{1 => 6, 2 => 16}\nCan@07:19:36, 15.4C, 81% humidity, 14.5psia, 15.628V, 0.153A, 0.153A avg, 2.39W",Subject: "TotaldeploymentFINAL begun" @07:19:37.16 Shutdown @07:19:37.20 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @07:19:37.22
Asleep til 20:12 10-september-19 @07:19:37.24 Retry #1 of 10 after 10:00 @07:19:37.28
Continuing at 20:12 @07:19:43.49 Session Begun @07:20:04.40 -> exit @07:20:04.42 Session Ended @07:20:07.21 Session Begun @07:20:16.56 -> exit @07:20:16.58 Session Ended @07:20:40.18 Session Begun @07:20:47.05 -> exit @07:20:47.07 Session Ended @07:21:17.36 Session Begun @07:21:21.91 -> exit @07:21:21.92 Session Ended @07:24:03.95 Session Begun @07:24:07.22 -> exit @07:24:07.23 Session Ended @07:24:22.89 Session Begun @07:24:28.50 -> showlog 10 @07:24:33.97 -> showlog 20 @07:24:52.77 -> exit @07:24:52.79 Session Ended @07:25:58.31 Session Begun @07:26:14.08 -> exit @07:26:14.10 Session Ended @07:28:23.62 Session Begun @07:28:39.76 -> abort! "trial" @07:28:39.78 CmdServer::Client::Error in mwj -- Aborting Mission: trial @07:28:49.11 -> exit @07:28:49.13 Session Ended @07:28:54.47 Session Begun @07:28:59.84
Sending Email @07:29:37.28 GoodNews.email "Starting TotaldeploymentFINAL w/pucks in tube 2\n{1 => 6, 2 => 16}\nCan@07:19:36, 15.4C, 81% humidity, 14.5psia, 15.628V, 0.153A, 0.153A avg, 2.39W",Subject: "TotaldeploymentFINAL begun" @07:29:37.33 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @07:29:37.37 Retry #2 of 10 after 10:00 @07:29:45.09 -> ls @07:29:45.10 NameError in mwj -- undefined local variable or method `ls' for # @07:30:02.34 -> exit @07:30:02.36 Session Ended @07:30:07.51 Session Begun @07:30:20.47 -> abort! "trial" @07:30:20.49 CmdServer::Client::Error in mwj -- Aborting Mission: trial @07:30:20.53 Email::Error in MAIN -- flush failed! @07:30:20.58 ThreadError in mwj -- killed thread @07:30:20.62 Session Ended @07:34:04.07CEST10-Sep-19
>RESET< ESP2 v3.30 I2C Gateway v3.06 for core bus @07:34:06.54 Copyright 2018 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @07:34:06.84 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @07:34:13.11 Polling Can every 10:00 @07:34:13.21 Cleanly exited at 07:30:20CEST10-Sep-19 @07:34:13.30 Loading /home/esp/esp2/mission/TotaldeploymentFINAL3.rb mission @07:34:13.46 Preparing to sleep until 20:48 10-september-19 @07:34:13.52 GoodNews.email "Starting TotaldeploymentFINAL3 w/pucks in tube 2\n{1 => 6, 2 => 16}\nCan@07:34:13, 15.5C, 81% humidity, 14.5psia, 15.613V, 0.159A, 0.159A avg, 2.48W",Subject: "TotaldeploymentFINAL3 begun" @07:34:13.66 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @07:34:13.78 Shutdown @07:34:13.82
Asleep til 20:48 10-september-19 @07:34:13.86 Retry #1 of 10 after 10:00 @07:34:13.93
Continuing at 20:48 @07:34:14.11 Session Begun @07:34:40.25 -> showlog 10 @07:34:46.08 -> exit @07:34:46.09 Session Ended @07:35:52.53 Session Begun @07:35:56.24 -> exit @07:35:56.25 Session Ended @07:36:20.58 Session Begun @07:36:35.72 -> showlog 20 @07:36:43.32 -> exit @07:36:43.33 Session Ended @07:44:13.92 GoodNews.email "Starting TotaldeploymentFINAL3 w/pucks in tube 2\n{1 => 6, 2 => 16}\nCan@07:34:13, 15.5C, 81% humidity, 14.5psia, 15.613V, 0.159A, 0.159A avg, 2.48W",Subject: "TotaldeploymentFINAL3 begun" @07:44:13.95 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @07:44:13.96 Retry #2 of 10 after 10:00 @07:54:13.99 GoodNews.email "Starting TotaldeploymentFINAL3 w/pucks in tube 2\n{1 => 6, 2 => 16}\nCan@07:34:13, 15.5C, 81% humidity, 14.5psia, 15.613V, 0.159A, 0.159A avg, 2.48W",Subject: "TotaldeploymentFINAL3 begun" @07:54:14.02 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @07:54:14.03 Retry #3 of 10 after 10:00 @08:04:14.06 GoodNews.email "Starting TotaldeploymentFINAL3 w/pucks in tube 2\n{1 => 6, 2 => 16}\nCan@07:34:13, 15.5C, 81% humidity, 14.5psia, 15.613V, 0.159A, 0.159A avg, 2.48W",Subject: "TotaldeploymentFINAL3 begun" @08:04:14.09 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @08:04:14.10 Retry #4 of 10 after 10:00 @08:14:14.13 GoodNews.email "Starting TotaldeploymentFINAL3 w/pucks in tube 2\n{1 => 6, 2 => 16}\nCan@07:34:13, 15.5C, 81% humidity, 14.5psia, 15.613V, 0.159A, 0.159A avg, 2.48W",Subject: "TotaldeploymentFINAL3 begun" @08:14:14.16 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @08:14:14.17 Retry #5 of 10 after 10:00 @08:24:14.20 GoodNews.email "Starting TotaldeploymentFINAL3 w/pucks in tube 2\n{1 => 6, 2 => 16}\nCan@07:34:13, 15.5C, 81% humidity, 14.5psia, 15.613V, 0.159A, 0.159A avg, 2.48W",Subject: "TotaldeploymentFINAL3 begun" @08:24:14.23 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @08:24:14.24 Retry #6 of 10 after 10:00 @08:34:14.27 GoodNews.email "Starting TotaldeploymentFINAL3 w/pucks in tube 2\n{1 => 6, 2 => 16}\nCan@07:34:13, 15.5C, 81% humidity, 14.5psia, 15.613V, 0.159A, 0.159A avg, 2.48W",Subject: "TotaldeploymentFINAL3 begun" @08:34:14.30 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @08:34:14.31 Retry #7 of 10 after 10:00 @08:44:14.34 GoodNews.email "Starting TotaldeploymentFINAL3 w/pucks in tube 2\n{1 => 6, 2 => 16}\nCan@07:34:13, 15.5C, 81% humidity, 14.5psia, 15.613V, 0.159A, 0.159A avg, 2.48W",Subject: "TotaldeploymentFINAL3 begun" @08:44:14.37 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @08:44:14.38 Retry #8 of 10 after 10:00 @08:54:14.41 GoodNews.email "Starting TotaldeploymentFINAL3 w/pucks in tube 2\n{1 => 6, 2 => 16}\nCan@07:34:13, 15.5C, 81% humidity, 14.5psia, 15.613V, 0.159A, 0.159A avg, 2.48W",Subject: "TotaldeploymentFINAL3 begun" @08:54:14.44 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @08:54:14.45 Retry #9 of 10 after 10:00 @09:04:14.48 GoodNews.email "Starting TotaldeploymentFINAL3 w/pucks in tube 2\n{1 => 6, 2 => 16}\nCan@07:34:13, 15.5C, 81% humidity, 14.5psia, 15.613V, 0.159A, 0.159A avg, 2.48W",Subject: "TotaldeploymentFINAL3 begun" @09:04:14.51 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @09:04:14.53 Retry #10 of 10 after 10:00 @09:14:14.55 GoodNews.email "Starting TotaldeploymentFINAL3 w/pucks in tube 2\n{1 => 6, 2 => 16}\nCan@07:34:13, 15.5C, 81% humidity, 14.5psia, 15.613V, 0.159A, 0.159A avg, 2.48W",Subject: "TotaldeploymentFINAL3 begun" @09:14:14.58 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @09:14:14.59 GoodNews.email "Then, in tube 2: mbaPhase\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:121\n{1 => 6, 2 => 16}\nCan@07:34:14, 15.5C, 81% humidity, 14.5psia, 15.613V, 0.159A, 0.165A avg, 2.48W",Subject: "Asleep until 20:48" @09:14:14.62 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @09:14:14.63 Retry #1 of 10 after 10:00 @09:24:14.66 GoodNews.email "Then, in tube 2: mbaPhase\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:121\n{1 => 6, 2 => 16}\nCan@07:34:14, 15.5C, 81% humidity, 14.5psia, 15.613V, 0.159A, 0.165A avg, 2.48W",Subject: "Asleep until 20:48" @09:24:14.69 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @09:24:14.70 Retry #2 of 10 after 10:00 @09:34:14.73 GoodNews.email "Then, in tube 2: mbaPhase\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:121\n{1 => 6, 2 => 16}\nCan@07:34:14, 15.5C, 81% humidity, 14.5psia, 15.613V, 0.159A, 0.165A avg, 2.48W",Subject: "Asleep until 20:48" @09:34:14.76 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @09:34:14.77 Retry #3 of 10 after 10:00 @09:44:14.80 GoodNews.email "Then, in tube 2: mbaPhase\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:121\n{1 => 6, 2 => 16}\nCan@07:34:14, 15.5C, 81% humidity, 14.5psia, 15.613V, 0.159A, 0.165A avg, 2.48W",Subject: "Asleep until 20:48" @09:44:14.86 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @09:44:14.90 Retry #4 of 10 after 10:00 @09:54:14.93 GoodNews.email "Then, in tube 2: mbaPhase\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:121\n{1 => 6, 2 => 16}\nCan@07:34:14, 15.5C, 81% humidity, 14.5psia, 15.613V, 0.159A, 0.165A avg, 2.48W",Subject: "Asleep until 20:48" @09:54:14.96 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @09:54:14.97 Retry #5 of 10 after 10:00 @10:04:15.00 GoodNews.email "Then, in tube 2: mbaPhase\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:121\n{1 => 6, 2 => 16}\nCan@07:34:14, 15.5C, 81% humidity, 14.5psia, 15.613V, 0.159A, 0.165A avg, 2.48W",Subject: "Asleep until 20:48" @10:04:15.03 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @10:04:15.04 Retry #6 of 10 after 10:00 @10:14:15.07 GoodNews.email "Then, in tube 2: mbaPhase\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:121\n{1 => 6, 2 => 16}\nCan@07:34:14, 15.5C, 81% humidity, 14.5psia, 15.613V, 0.159A, 0.165A avg, 2.48W",Subject: "Asleep until 20:48" @10:14:15.10 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @10:14:15.11 Retry #7 of 10 after 10:00 @10:24:15.14 GoodNews.email "Then, in tube 2: mbaPhase\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:121\n{1 => 6, 2 => 16}\nCan@07:34:14, 15.5C, 81% humidity, 14.5psia, 15.613V, 0.159A, 0.165A avg, 2.48W",Subject: "Asleep until 20:48" @10:24:15.17 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @10:24:15.18 Retry #8 of 10 after 10:00 @10:34:15.21 GoodNews.email "Then, in tube 2: mbaPhase\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:121\n{1 => 6, 2 => 16}\nCan@07:34:14, 15.5C, 81% humidity, 14.5psia, 15.613V, 0.159A, 0.165A avg, 2.48W",Subject: "Asleep until 20:48" @10:34:15.24 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @10:34:15.25 Retry #9 of 10 after 10:00 @10:44:15.28 GoodNews.email "Then, in tube 2: mbaPhase\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:121\n{1 => 6, 2 => 16}\nCan@07:34:14, 15.5C, 81% humidity, 14.5psia, 15.613V, 0.159A, 0.165A avg, 2.48W",Subject: "Asleep until 20:48" @10:44:15.31 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @10:44:15.32 Retry #10 of 10 after 10:00 @10:54:15.35 GoodNews.email "Then, in tube 2: mbaPhase\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:121\n{1 => 6, 2 => 16}\nCan@07:34:14, 15.5C, 81% humidity, 14.5psia, 15.613V, 0.159A, 0.165A avg, 2.48W",Subject: "Asleep until 20:48" @10:54:15.38 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @20:48:00.05
Powering core bus @20:48:04.91 Gate.power :raw,:ON @20:48:05.58 SPE.coast @20:48:05.65 SPE.configure SPEconfig @20:48:05.74 AS.configure ASconfig @20:48:05.85 SS.configure SSconfig @20:48:05.96 CC.configure CCconfig @20:48:06.05 CS.configure CSconfig @20:48:06.15 PC.configure PCconfig @20:48:06.25 PS.configure PSconfig @20:48:06.37 Forearm.configure LooseForearm @20:48:06.46 Elbow.configure LooseElbow @20:48:06.58 SC.configure SCloose @20:48:06.68 SE.configure SEconfig @20:48:06.73 CH.coast @20:48:06.78 CH.configure CHconfig @20:48:06.82 PH.coast @20:48:06.88 PH.configure PHconfig @20:48:06.92 PCR.off @20:48:06.95 PCR.pidtemp= 50,0.1,0,300,25,25 @20:48:06.98 PCR.caltemp= 2.8814,0.0372,0 @20:48:07.00 All dwarf controllers running firmware version 3.75 @20:48:07.12 Valve.reset @20:48:07.29 PCR.off @20:48:07.33 PCR.pidtemp= 50,0.1,0,300,25,25 @20:48:07.36 PCR.caltemp= 2.8814,0.0372,0 @20:48:07.55 Intake.select :close @20:48:07.60 Exhaust.select :close @20:48:07.61 CTV.dial :air @20:48:07.70 PSV.dial :air @20:48:07.73 CSV.dial :waste1 @20:48:07.94
Forearm.reconfigure LooseForearm @20:48:07.96 PS.reconfigure PSconfig @20:48:08.07 PS.home.seek 0ml @20:48:08.09
Forearm.home.seek :retract @20:48:08.18 SSV.dial :spit,avoiding: :exhaust @20:48:08.34 CS.reconfigure CSfast @20:48:08.37 SS.reconfigure SSconfig @20:48:08.47 CS.home.seek 0ml @20:48:08.50 SS.home.seek 0ml @20:48:08.90 Hand.close @20:48:08.98
Elbow.jog 1500 @20:48:09.42 Elbow.reconfigure LooseElbow @20:48:09.47 Elbow.home.seek :clear @20:48:14.00 SE.reconfigure SEconfig @20:48:14.05 SE.home.seek :down @20:48:14.76 SC.reconfigure SCsloppy @20:48:14.81 SC.home.seek :initial @20:48:17.04 PS.coast @20:48:18.75
Forearm.seek :retract @20:48:18.83 Elbow.seek :hammer @20:48:20.07 Forearm.reconfigure TightForearm @20:48:20.12 Forearm.seek 5 + 350 counts @20:48:21.56 Forearm.seek :retract @20:48:22.88 CC.reconfigure CCconfig @20:48:22.90 PC.reconfigure PCconfig @20:48:23.01
CC.home.seek :open @20:48:23.03 PC.home.seek :open @20:48:26.40
Phase [mbaPhase] @20:48:26.42 GoodNews.email "At 20:48:26 began in tube 2: mbaPhase\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:121\n{1 => 6, 2 => 16}\nCan@20:48:26, 16.6C, 79% humidity, 14.6psia, 15.186V, 0.809A, 0.809A avg, 12.28W",Subject: "Started TotaldeploymentFINAL3 phase mbaPhase" @20:48:26.44CEST10-Sep-19
==================> STARTED Superphase (phase #1) <=== @20:48:26.48 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @20:48:26.50 Starting initialPurge @20:48:26.52 Retry #1 of 10 after 10:00 @20:48:26.54
Loading FlushPuck into Collection Clamp from FlushPuck::Garage @20:48:26.63 Hand.open @20:48:27.33 Elbow.reconfigure TightElbow @20:48:27.38 Elbow.seek FlushPuck::Garage @20:48:29.23 Elbow.reconfigure LooseElbow @20:48:29.34 Forearm.reconfigure LooseForearm @20:48:29.39 Forearm.seek :garage @20:48:30.35 Hand.close @20:48:31.09 Forearm.seek :retract @20:48:32.10 Elbow.reconfigure TightElbow @20:48:32.15 Elbow.seek CC @20:48:35.09 Elbow.reconfigure LooseElbow @20:48:35.20 Forearm.reconfigure TightForearm @20:48:35.25 Forearm.seek CC @20:48:36.60 CC.seek :closed @20:48:40.85 Collection Clamp onSpring/offSpring for 6/32 samples, on/off torque 1.62 @20:48:40.94 Hand.open @20:48:41.65 Forearm.reconfigure LooseForearm @20:48:41.69 Forearm.seek :retract @20:48:42.68 Flushing the Collection Valve::Manifold @20:48:42.82 CSV.dial CSR @20:48:44.09 Pulling 5ml of CSR.flush @20:48:44.10 CSR.select :flush @20:48:44.22 CS.reconfigure CSconfig @20:48:44.27 CS.seek 5ml @20:48:58.00 delay 5 seconds @20:49:03.03 CSR.select :air @20:49:03.19 CS.reconfigure CSfast @20:49:03.24 CS.seek 7.5ml @20:49:09.56 CSV.dial :waste @20:49:10.76 CS.reconfigure CSconfig @20:49:10.81 CS.seek 0ml @20:49:30.96 Purging external Intake @20:49:31.09 CSV.dial :flush @20:49:31.54 CS.reconfigure CSfast @20:49:31.59 CS.seek 10ml @20:49:52.97 delay 5 seconds @20:49:58.00 CSV.dial CBV @20:49:58.03
CBV.dial CSV @20:49:58.78 CTV.dial :intake @20:49:59.51 Intake.select :open @20:49:59.63 CS.seek 0ml @20:50:22.40 Intake.select :close @20:50:22.51 CTV.dial :air @20:50:23.22 Flushing upper manifold @20:50:23.35 CSV.dial :flush @20:50:24.05 CS.seek 5ml @20:50:35.31 delay 5 seconds @20:50:40.34 CSV.dial :air @20:50:40.97 CS.seek 7ml @20:50:46.28 CSV.dial CBV @20:50:46.30
CBV.dial CSV @20:50:47.28 CTV.dial :air @20:50:47.30 CS.seek 0ml @20:51:02.59 delay 7 seconds @20:51:09.63 CS.seek 3ml @20:51:16.81 CSV.dial :waste @20:51:17.26 CS.seek 0ml @20:51:24.55 CSV.dial :flush @20:51:24.99 CS.seek 3ml @20:51:32.25 delay 5 seconds @20:51:37.28 CSV.dial :air @20:51:37.79 CS.seek 5.001ml @20:51:43.00 CSV.dial :air @20:51:43.02 CS.seek 0ml @20:51:54.31 delay 7 seconds @20:52:01.35 CS.seek 3ml @20:52:08.61 CSV.dial :waste @20:52:09.34 CS.seek 0ml @20:52:16.50 Garaging FlushPuck in Collection Clamp @20:52:16.58 Elbow.reconfigure TightElbow @20:52:16.63 Elbow.seek CC @20:52:16.89 Elbow.reconfigure LooseElbow @20:52:17.00 Forearm.seek CC @20:52:18.02 Hand.close @20:52:18.58 CC.seek :open @20:52:23.41 Forearm.seek :retract @20:52:24.50 Elbow.reconfigure TightElbow @20:52:24.55 Elbow.seek :garage @20:52:27.62 Elbow.reconfigure LooseElbow @20:52:27.72 Forearm.reconfigure TightForearm @20:52:27.77 Forearm.seek :garage @20:52:28.95 Hand.open @20:52:29.65 Forearm.reconfigure LooseForearm @20:52:29.70 Forearm.seek :retract @20:52:30.78 Starting mfb @20:52:30.80 pcrBAC sampling at most 2000ml, wcr at most 2000ml @20:52:30.89 Loading sh1 puck from tube 2 into Collection Clamp @20:52:30.98 Readying the MFB @20:52:31.05 Homing MFB Syringe @20:52:31.06 AFV.select :holdingCoil @20:52:31.30 ATV.dial :air2 @20:52:31.42
SC.reconfigure SCloose @20:52:31.66 Elbow.reconfigure TightElbow @20:52:31.71 Elbow.seek 2 @20:52:32.52 AS.reconfigure ASconfig @20:52:32.57 AS.home.seek :home @20:52:33.75
Forearm.reconfigure TightForearm @20:52:33.80 Forearm.seek 2 + 700 counts @20:52:34.80 SE.reconfigure SEpick @20:52:34.88 SE.seek beamBreak @20:52:36.39 SE.configure SEconfig @20:52:36.57 Elbow.seek 2 @20:52:36.83 Forearm.seek 2 + 300 counts @20:52:37.08 Forearm.reconfigure LooseForearm @20:52:37.13 Hand.close @20:52:37.72 SE.seek :down @20:52:37.96
Forearm.seek :retract @20:52:38.15 AFV.select :carrierSolution @20:52:38.26 delay 2 seconds @20:52:38.89
Elbow.seek CC @20:52:39.09 SE.coast @20:52:40.29 AS.seek 1ml @20:52:40.51
Elbow.reconfigure LooseElbow @20:52:40.62 Forearm.reconfigure TightForearm @20:52:40.67 Forearm.seek CC @20:52:42.03 CC.seek :closed @20:52:46.31 Collection Clamp onSpring/offSpring for 6/32 samples, on/off torque 1.67 @20:52:46.32 Hand.open @20:52:47.02 Forearm.reconfigure LooseForearm @20:52:47.07 Forearm.seek :retract @20:52:48.08 SC.coast @20:52:48.12 collectSample 2000ml w/bubble point of 55psi @20:52:48.13 Priming 7ml @20:52:48.14 Intake.select :close @20:52:48.15 Exhaust.select :close @20:52:48.17 SS.hold @20:52:48.40 Calibrating pressure and temperature sensors @20:52:48.42 CTV.dial :air @20:52:48.43 SSV.dial :spit,avoiding: :exhaust @20:52:48.45 SS.reconfigure SSfast @20:52:48.50 SS.seek 5ml @20:52:51.25 SSV.dial CBV @20:52:51.33
CBV.dial SSV @20:52:52.58 delay 3.8 seconds @20:52:56.53 SSV.dial :spit @20:52:58.27 SS.seek 0ml @20:53:00.69 SSV.dial CBV @20:53:01.94 CTV.dial :intake @20:53:02.68 SSV.dial CBV @20:53:02.70
CBV.dial SSV @20:53:02.72 Allowing 40s for 7.0ml of remaining 7.0ml @20:53:02.73 Intake.select :open @20:53:04.92 Intake @32.0psia, vacuum from 20.0 to 24.0psi @20:53:04.94 SS.reconfigure SSinhale.with maxSpeed: 150 @20:53:04.98 SS.seek 7ml @20:53:08.58 Vacuum too high -- waiting up to 40s @20:53:10.74 Intake @31.4psia, vacuum from 20.0 to 24.0psi @20:53:10.75 SS.reconfigure SSinhale.with maxSpeed: 135 @20:53:10.80 SS.seek 7ml @20:53:13.70 Stroke completed -- waiting for pressure to equalize ... @20:53:17.55 Intake.select :close @20:53:17.71 SSV.dial :exhaust @20:53:18.44 Exhaust.select :open @20:53:18.56 SS.reconfigure SSexhale @20:53:18.60 SS.seek 0ml @20:53:22.03 Exhaust.select :close @20:53:22.14 Sampling 2000.0ml @20:53:22.15 Intake.select :close @20:53:22.17 Exhaust.select :close @20:53:22.18 SS.reconfigure SSconfig @20:53:22.22 SS.hold @20:53:22.45 CTV.dial :intake @20:53:22.46 SSV.dial CBV @20:53:22.48
CBV.dial SSV @20:53:23.23 Allowing 130s for 25.0ml of remaining 2000.0ml @20:53:23.24 Intake.select :open @20:53:26.43 Intake @32.0psia, vacuum from 20.0 to 24.0psi @20:53:26.45 SS.reconfigure SSinhale.with maxSpeed: 135 @20:53:26.50 SS.seek 25ml @20:53:30.46 Vacuum too high -- waiting up to 40s @20:53:32.31 Intake @31.3psia, vacuum from 20.0 to 24.0psi @20:53:32.32 SS.reconfigure SSinhale.with maxSpeed: 122 @20:53:32.37 SS.seek 25ml @20:53:33.21 delay 1 second @20:53:34.23 AFV.select :holdingCoil @20:53:34.33 delay 1 second @20:53:35.35 ATV.dial :waste @20:53:35.61
Vacuum too high -- waiting up to 40s @20:53:36.50 AS.reconfigure ASnorm @20:53:36.55 AS.home.seek :empty @20:53:37.57
Intake @31.4psia, vacuum from 20.0 to 24.0psi @20:53:37.58 SS.reconfigure SSinhale.with maxSpeed: 110 @20:53:37.63 SS.seek 25ml @20:53:40.64 Vacuum too high -- waiting up to 40s @20:53:42.23 Intake @31.2psia, vacuum from 20.0 to 24.0psi @20:53:42.25 SS.reconfigure SSinhale.with maxSpeed: 99 @20:53:42.30 SS.seek 25ml @20:53:46.58 Vacuum too high -- waiting up to 40s @20:53:48.21 Intake @31.4psia, vacuum from 20.0 to 24.0psi @20:53:48.22 SS.reconfigure SSinhale.with maxSpeed: 89 @20:53:48.27 SS.seek 25ml @20:53:52.02 Vacuum too high -- waiting up to 40s @20:53:53.64 Intake @31.4psia, vacuum from 20.0 to 24.0psi @20:53:53.65 SS.reconfigure SSinhale.with maxSpeed: 80 @20:53:53.70 SS.seek 25ml @20:53:57.95 Vacuum too high -- waiting up to 40s @20:53:59.56 Intake @31.5psia, vacuum from 20.0 to 24.0psi @20:53:59.58 SS.reconfigure SSinhale.with maxSpeed: 72 @20:53:59.63 SS.seek 25ml @20:54:02.27 Stroke completed -- waiting for pressure to equalize ... @20:54:04.88 Intake.select :close @20:54:05.05 SSV.dial :exhaust @20:54:05.81 Exhaust.select :open @20:54:05.93 SS.reconfigure SSexhale @20:54:05.97 SS.seek 0ml @20:54:14.81 Exhaust.select :close @20:54:14.93 SSV.dial CBV @20:54:14.95
CBV.dial SSV @20:54:15.72 Allowing 130s for 25.0ml of remaining 1975.0ml @20:54:15.73 Intake.select :open @20:54:17.91 Intake @32.0psia, vacuum from 20.0 to 24.0psi @20:54:17.92 SS.reconfigure SSinhale.with maxSpeed: 72 @20:54:17.97 SS.seek 25ml @20:54:24.24 Vacuum too high -- waiting up to 40s @20:54:25.61 Intake @31.4psia, vacuum from 20.0 to 24.0psi @20:54:25.62 SS.reconfigure SSinhale.with maxSpeed: 65 @20:54:25.67 SS.seek 25ml @20:54:29.12 ATV.dial :waste @20:54:29.13 ARV.dial between carrier and air1 @20:54:29.22 ASV.dial :waste1 @20:54:33.98
Vacuum too high -- waiting up to 40s @20:54:35.30 Intake @31.3psia, vacuum from 20.0 to 24.0psi @20:54:35.32 SS.reconfigure SSinhale.with maxSpeed: 59 @20:54:35.37 SS.seek 25ml @20:54:50.78 Stroke completed -- waiting for pressure to equalize ... @20:54:53.99 Intake.select :close @20:54:54.15 SSV.dial :exhaust @20:54:54.89 Exhaust.select :open @20:54:55.01 SS.reconfigure SSexhale @20:54:55.05 SS.seek 0ml @20:55:03.58 Exhaust.select :close @20:55:03.69 SSV.dial CBV @20:55:04.27
CBV.dial SSV @20:55:05.06 Allowing 130s for 25.0ml of remaining 1950.0ml @20:55:05.07 Intake.select :open @20:55:07.25 Intake @32.0psia, vacuum from 20.0 to 24.0psi @20:55:07.26 SS.reconfigure SSinhale.with maxSpeed: 59 @20:55:07.32 SS.seek 25ml @20:55:21.80 Vacuum too high -- waiting up to 40s @20:55:22.90 Intake @31.3psia, vacuum from 20.0 to 24.0psi @20:55:22.91 SS.reconfigure SSinhale.with maxSpeed: 53 @20:55:22.96 SS.seek 25ml @20:55:41.56 Stroke completed -- waiting for pressure to equalize ... @20:55:44.17 Intake.select :close @20:55:44.32 SSV.dial :exhaust @20:55:45.05 Exhaust.select :open @20:55:45.16 SS.reconfigure SSexhale @20:55:45.21 SS.seek 0ml @20:55:54.00 Exhaust.select :close @20:55:54.11 SSV.dial CBV @20:55:54.14
CBV.dial SSV @20:55:54.89 Allowing 130s for 25.0ml of remaining 1925.0ml @20:55:54.90 Intake.select :open @20:55:57.08 Intake @32.0psia, vacuum from 20.0 to 24.0psi @20:55:57.09 SS.reconfigure SSinhale.with maxSpeed: 53 @20:55:57.14 SS.seek 25ml @20:56:30.96 Stroke completed -- waiting for pressure to equalize ... @20:56:34.34 Intake.select :close @20:56:34.58 SSV.dial :exhaust @20:56:35.34 Exhaust.select :open @20:56:35.46 SS.reconfigure SSexhale @20:56:35.50 SS.seek 0ml @20:56:44.29 Exhaust.select :close @20:56:44.40 SSV.dial CBV @20:56:44.43
CBV.dial SSV @20:56:45.19 Allowing 130s for 25.0ml of remaining 1900.0ml @20:56:45.20 Intake.select :open @20:56:47.38 Intake @32.2psia, vacuum from 20.0 to 24.0psi @20:56:47.39 SS.reconfigure SSinhale.with maxSpeed: 53 @20:56:47.44 SS.seek 25ml @20:57:21.30 Stroke completed -- waiting for pressure to equalize ... @20:57:24.52 Intake.select :close @20:57:24.68 SSV.dial :exhaust @20:57:25.40 Exhaust.select :open @20:57:25.51 SS.reconfigure SSexhale @20:57:25.56 SS.seek 0ml @20:57:34.12 Exhaust.select :close @20:57:34.24 SSV.dial CBV @20:57:34.26
CBV.dial SSV @20:57:35.01 Allowing 130s for 25.0ml of remaining 1875.0ml @20:57:35.02 Intake.select :open @20:57:37.20 Intake @32.1psia, vacuum from 20.0 to 24.0psi @20:57:37.21 SS.reconfigure SSinhale.with maxSpeed: 53 @20:57:37.26 SS.seek 25ml @20:58:11.34 Stroke completed -- waiting for pressure to equalize ... @20:58:14.53 Intake.select :close @20:58:14.69 SSV.dial :exhaust @20:58:15.45 Exhaust.select :open @20:58:15.57 SS.reconfigure SSexhale @20:58:15.61 SS.seek 0ml @20:58:24.37 Exhaust.select :close @20:58:24.49 SSV.dial CBV @20:58:24.51
CBV.dial SSV @20:58:25.28 Allowing 130s for 25.0ml of remaining 1850.0ml @20:58:25.29 Intake.select :open @20:58:26.54 GoodNews.email "At 20:48:26 began in tube 2: mbaPhase\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:121\n{1 => 6, 2 => 16}\nCan@20:48:26, 16.6C, 79% humidity, 14.6psia, 15.186V, 0.809A, 0.809A avg, 12.28W",Subject: "Started TotaldeploymentFINAL3 phase mbaPhase" @20:58:26.57 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @20:58:26.58 Retry #2 of 10 after 10:00 @20:58:27.46
Intake @32.2psia, vacuum from 20.0 to 24.0psi @20:58:27.48 SS.reconfigure SSinhale.with maxSpeed: 53 @20:58:27.53 SS.seek 25ml @20:59:01.41 Stroke completed -- waiting for pressure to equalize ... @20:59:04.49 Intake.select :close @20:59:04.65 SSV.dial :exhaust @20:59:05.39 Exhaust.select :open @20:59:05.51 SS.reconfigure SSexhale @20:59:05.55 SS.seek 0ml @20:59:14.25 Exhaust.select :close @20:59:14.37 SSV.dial CBV @20:59:14.39
CBV.dial SSV @20:59:15.17 Allowing 130s for 25.0ml of remaining 1825.0ml @20:59:15.18 Intake.select :open @20:59:17.35 Intake @32.1psia, vacuum from 20.0 to 24.0psi @20:59:17.37 SS.reconfigure SSinhale.with maxSpeed: 53 @20:59:17.42 SS.seek 25ml @20:59:51.41 Stroke completed -- waiting for pressure to equalize ... @20:59:54.66 Intake.select :close @20:59:54.81 SSV.dial :exhaust @20:59:55.54 Exhaust.select :open @20:59:55.66 SS.reconfigure SSexhale @20:59:55.70 SS.seek 0ml @21:00:04.54 Exhaust.select :close @21:00:04.66 SSV.dial CBV @21:00:04.68
CBV.dial SSV @21:00:05.43 Allowing 130s for 25.0ml of remaining 1800.0ml @21:00:05.44 Intake.select :open @21:00:07.62 Intake @32.1psia, vacuum from 20.0 to 24.0psi @21:00:07.63 SS.reconfigure SSinhale.with maxSpeed: 53 @21:00:07.69 SS.seek 25ml @21:00:41.58 Stroke completed -- waiting for pressure to equalize ... @21:00:44.80 Intake.select :close @21:00:44.96 SSV.dial :exhaust @21:00:45.68 Exhaust.select :open @21:00:45.80 SS.reconfigure SSexhale @21:00:45.84 SS.seek 0ml @21:00:54.37 Exhaust.select :close @21:00:54.48 SSV.dial CBV @21:00:54.52
CBV.dial SSV @21:00:55.26 Allowing 130s for 25.0ml of remaining 1775.0ml @21:00:55.27 Intake.select :open @21:00:57.45 Intake @32.3psia, vacuum from 20.0 to 24.0psi @21:00:57.46 SS.reconfigure SSinhale.with maxSpeed: 53 @21:00:57.51 SS.seek 25ml @21:01:28.35 Vacuum too high -- waiting up to 40s @21:01:29.44 Intake @31.4psia, vacuum from 20.0 to 24.0psi @21:01:29.46 SS.reconfigure SSinhale.with maxSpeed: 48 @21:01:29.51 SS.seek 25ml @21:01:33.51 Stroke completed -- waiting for pressure to equalize ... @21:01:36.11 Intake.select :close @21:01:36.27 SSV.dial :exhaust @21:01:37.00 Exhaust.select :open @21:01:37.11 SS.reconfigure SSexhale @21:01:37.16 SS.seek 0ml @21:01:45.81 Exhaust.select :close @21:01:45.92 SSV.dial CBV @21:01:45.95
CBV.dial SSV @21:01:46.70 Allowing 130s for 25.0ml of remaining 1750.0ml @21:01:46.71 Intake.select :open @21:01:48.89 Intake @32.2psia, vacuum from 20.0 to 24.0psi @21:01:48.90 SS.reconfigure SSinhale.with maxSpeed: 48 @21:01:48.95 SS.seek 25ml @21:02:26.42 Stroke completed -- waiting for pressure to equalize ... @21:02:29.62 Intake.select :close @21:02:29.78 SSV.dial :exhaust @21:02:30.54 Exhaust.select :open @21:02:30.65 SS.reconfigure SSexhale @21:02:30.70 SS.seek 0ml @21:02:39.63 Exhaust.select :close @21:02:39.85 SSV.dial CBV @21:02:39.87
CBV.dial SSV @21:02:40.64 Allowing 130s for 25.0ml of remaining 1725.0ml @21:02:40.65 Intake.select :open @21:02:42.83 Intake @32.2psia, vacuum from 20.0 to 24.0psi @21:02:42.85 SS.reconfigure SSinhale.with maxSpeed: 48 @21:02:42.90 SS.seek 25ml @21:03:20.31 Stroke completed -- waiting for pressure to equalize ... @21:03:23.55 Intake.select :close @21:03:23.71 SSV.dial :exhaust @21:03:24.43 Exhaust.select :open @21:03:24.55 SS.reconfigure SSexhale @21:03:24.59 SS.seek 0ml @21:03:33.14 Exhaust.select :close @21:03:33.25 SSV.dial CBV @21:03:33.28
CBV.dial SSV @21:03:34.06 Allowing 130s for 25.0ml of remaining 1700.0ml @21:03:34.09 Intake.select :open @21:03:36.31 Intake @32.2psia, vacuum from 20.0 to 24.0psi @21:03:36.32 SS.reconfigure SSinhale.with maxSpeed: 48 @21:03:36.37 SS.seek 25ml @21:04:13.86 Stroke completed -- waiting for pressure to equalize ... @21:04:17.07 Intake.select :close @21:04:17.23 SSV.dial :exhaust @21:04:17.95 Exhaust.select :open @21:04:18.07 SS.reconfigure SSexhale @21:04:18.11 SS.seek 0ml @21:04:26.90 Exhaust.select :close @21:04:27.02 SSV.dial CBV @21:04:27.04
CBV.dial SSV @21:04:27.79 Allowing 130s for 25.0ml of remaining 1675.0ml @21:04:27.80 Intake.select :open @21:04:29.97 Intake @32.3psia, vacuum from 20.0 to 24.0psi @21:04:29.99 SS.reconfigure SSinhale.with maxSpeed: 48 @21:04:30.04 SS.seek 25ml @21:05:07.34 Stroke completed -- waiting for pressure to equalize ... @21:05:10.54 Intake.select :close @21:05:10.70 SSV.dial :exhaust @21:05:11.43 Exhaust.select :open @21:05:11.55 SS.reconfigure SSexhale @21:05:11.59 SS.seek 0ml @21:05:20.15 Exhaust.select :close @21:05:20.26 SSV.dial CBV @21:05:20.29
CBV.dial SSV @21:05:21.04 Allowing 130s for 25.0ml of remaining 1650.0ml @21:05:21.05 Intake.select :open @21:05:23.23 Intake @32.1psia, vacuum from 20.0 to 24.0psi @21:05:23.24 SS.reconfigure SSinhale.with maxSpeed: 48 @21:05:23.29 SS.seek 25ml @21:06:00.70 Stroke completed -- waiting for pressure to equalize ... @21:06:03.93 Intake.select :close @21:06:04.09 SSV.dial :exhaust @21:06:04.82 Exhaust.select :open @21:06:04.94 SS.reconfigure SSexhale @21:06:04.98 SS.seek 0ml @21:06:13.86 Exhaust.select :close @21:06:13.98 SSV.dial CBV @21:06:14.00
CBV.dial SSV @21:06:14.76 Allowing 130s for 25.0ml of remaining 1625.0ml @21:06:14.77 Intake.select :open @21:06:17.34 Intake @32.2psia, vacuum from 20.0 to 24.0psi @21:06:17.44 SS.reconfigure SSinhale.with maxSpeed: 48 @21:06:17.50 SS.seek 25ml @21:06:54.64 Stroke completed -- waiting for pressure to equalize ... @21:06:57.88 Intake.select :close @21:06:58.04 SSV.dial :exhaust @21:06:58.76 Exhaust.select :open @21:06:58.88 SS.reconfigure SSexhale @21:06:58.92 SS.seek 0ml @21:07:07.93 Exhaust.select :close @21:07:08.05 SSV.dial CBV @21:07:08.07
CBV.dial SSV @21:07:08.82 Allowing 130s for 25.0ml of remaining 1600.0ml @21:07:08.83 Intake.select :open @21:07:11.00 Intake @32.3psia, vacuum from 20.0 to 24.0psi @21:07:11.02 SS.reconfigure SSinhale.with maxSpeed: 48 @21:07:11.07 SS.seek 25ml @21:07:47.78 Stroke completed -- waiting for pressure to equalize ... @21:07:51.01 Intake.select :close @21:07:51.17 SSV.dial :exhaust @21:07:51.92 Exhaust.select :open @21:07:52.04 SS.reconfigure SSexhale @21:07:52.08 SS.seek 0ml @21:08:00.89 Exhaust.select :close @21:08:01.00 SSV.dial CBV @21:08:01.03
CBV.dial SSV @21:08:01.81 Allowing 130s for 25.0ml of remaining 1575.0ml @21:08:01.82 Intake.select :open @21:08:04.00 Intake @32.3psia, vacuum from 20.0 to 24.0psi @21:08:04.01 SS.reconfigure SSinhale.with maxSpeed: 48 @21:08:04.06 SS.seek 25ml @21:08:26.62 GoodNews.email "At 20:48:26 began in tube 2: mbaPhase\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:121\n{1 => 6, 2 => 16}\nCan@20:48:26, 16.6C, 79% humidity, 14.6psia, 15.186V, 0.809A, 0.809A avg, 12.28W",Subject: "Started TotaldeploymentFINAL3 phase mbaPhase" @21:08:26.65 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @21:08:26.66 Retry #3 of 10 after 10:00 @21:08:41.20
Stroke completed -- waiting for pressure to equalize ... @21:08:44.42 Intake.select :close @21:08:44.58 SSV.dial :exhaust @21:08:45.31 Exhaust.select :open @21:08:45.43 SS.reconfigure SSexhale @21:08:45.47 SS.seek 0ml @21:08:54.45 Exhaust.select :close @21:08:54.56 SSV.dial CBV @21:08:54.61
CBV.dial SSV @21:08:55.32 Allowing 130s for 25.0ml of remaining 1550.0ml @21:08:55.33 Intake.select :open @21:08:57.51 Intake @32.4psia, vacuum from 20.0 to 24.0psi @21:08:57.52 SS.reconfigure SSinhale.with maxSpeed: 48 @21:08:57.57 SS.seek 25ml @21:09:34.28 Stroke completed -- waiting for pressure to equalize ... @21:09:37.48 Intake.select :close @21:09:37.64 SSV.dial :exhaust @21:09:38.37 Exhaust.select :open @21:09:38.49 SS.reconfigure SSexhale @21:09:38.53 SS.seek 0ml @21:09:47.31 Exhaust.select :close @21:09:47.43 SSV.dial CBV @21:09:47.45
CBV.dial SSV @21:09:48.20 Allowing 130s for 25.0ml of remaining 1525.0ml @21:09:48.21 Intake.select :open @21:09:50.38 Intake @32.4psia, vacuum from 20.0 to 24.0psi @21:09:50.40 SS.reconfigure SSinhale.with maxSpeed: 48 @21:09:50.45 SS.seek 25ml @21:10:27.47 Stroke completed -- waiting for pressure to equalize ... @21:10:31.20 Intake.select :close @21:10:31.36 SSV.dial :exhaust @21:10:32.09 Exhaust.select :open @21:10:32.21 SS.reconfigure SSexhale @21:10:32.25 SS.seek 0ml @21:10:40.81 Exhaust.select :close @21:10:40.93 SSV.dial CBV @21:10:40.95
CBV.dial SSV @21:10:41.71 Allowing 130s for 25.0ml of remaining 1500.0ml @21:10:41.72 Intake.select :open @21:10:43.90 Intake @32.3psia, vacuum from 20.0 to 24.0psi @21:10:43.91 SS.reconfigure SSinhale.with maxSpeed: 48 @21:10:43.97 SS.seek 25ml @21:11:17.27 Vacuum too high -- waiting up to 40s @21:11:18.35 Intake @31.8psia, vacuum from 20.0 to 24.0psi @21:11:18.37 SS.reconfigure SSinhale.with maxSpeed: 43 @21:11:18.42 SS.seek 25ml @21:11:23.15 Stroke completed -- waiting for pressure to equalize ... @21:11:25.75 Intake.select :close @21:11:25.90 SSV.dial :exhaust @21:11:26.62 Exhaust.select :open @21:11:26.74 SS.reconfigure SSexhale @21:11:26.78 SS.seek 0ml @21:11:35.82 Exhaust.select :close @21:11:35.94 SSV.dial CBV @21:11:35.96
CBV.dial SSV @21:11:36.71 Allowing 130s for 25.0ml of remaining 1475.0ml @21:11:36.72 Intake.select :open @21:11:38.90 Intake @32.4psia, vacuum from 20.0 to 24.0psi @21:11:38.91 SS.reconfigure SSinhale.with maxSpeed: 43 @21:11:38.96 SS.seek 25ml @21:12:20.08 Stroke completed -- waiting for pressure to equalize ... @21:12:22.66 Intake.select :close @21:12:22.82 SSV.dial :exhaust @21:12:23.54 Exhaust.select :open @21:12:23.66 SS.reconfigure SSexhale @21:12:23.70 SS.seek 0ml @21:12:32.67 Exhaust.select :close @21:12:32.78 SSV.dial CBV @21:12:32.81
CBV.dial SSV @21:12:33.59 Allowing 130s for 25.0ml of remaining 1450.0ml @21:12:33.60 Intake.select :open @21:12:35.78 Intake @32.2psia, vacuum from 20.0 to 24.0psi @21:12:35.79 SS.reconfigure SSinhale.with maxSpeed: 43 @21:12:35.84 SS.seek 25ml @21:13:17.09 Stroke completed -- waiting for pressure to equalize ... @21:13:20.29 Intake.select :close @21:13:20.45 SSV.dial :exhaust @21:13:21.20 Exhaust.select :open @21:13:21.31 SS.reconfigure SSexhale @21:13:21.36 SS.seek 0ml @21:13:30.26 Exhaust.select :close @21:13:30.37 SSV.dial CBV @21:13:30.40
CBV.dial SSV @21:13:31.17 Allowing 130s for 25.0ml of remaining 1425.0ml @21:13:31.18 Intake.select :open @21:13:33.35 Intake @32.4psia, vacuum from 20.0 to 24.0psi @21:13:33.37 SS.reconfigure SSinhale.with maxSpeed: 43 @21:13:33.42 SS.seek 25ml @21:14:14.99 Stroke completed -- waiting for pressure to equalize ... @21:14:18.33 Intake.select :close @21:14:18.49 SSV.dial :exhaust @21:14:19.21 Exhaust.select :open @21:14:19.33 SS.reconfigure SSexhale @21:14:19.37 SS.seek 0ml @21:14:28.32 Exhaust.select :close @21:14:28.44 SSV.dial CBV @21:14:28.46
CBV.dial SSV @21:14:29.21 Allowing 130s for 25.0ml of remaining 1400.0ml @21:14:29.22 Intake.select :open @21:14:31.40 Intake @32.5psia, vacuum from 20.0 to 24.0psi @21:14:31.41 SS.reconfigure SSinhale.with maxSpeed: 43 @21:14:31.46 SS.seek 25ml @21:15:12.73 Stroke completed -- waiting for pressure to equalize ... @21:15:15.97 Intake.select :close @21:15:16.13 SSV.dial :exhaust @21:15:16.85 Exhaust.select :open @21:15:16.97 SS.reconfigure SSexhale @21:15:17.01 SS.seek 0ml @21:15:25.78 Exhaust.select :close @21:15:25.90 SSV.dial CBV @21:15:25.92
CBV.dial SSV @21:15:26.67 Allowing 130s for 25.0ml of remaining 1375.0ml @21:15:26.68 Intake.select :open @21:15:28.85 Intake @32.4psia, vacuum from 20.0 to 24.0psi @21:15:28.87 SS.reconfigure SSinhale.with maxSpeed: 43 @21:15:28.92 SS.seek 25ml @21:16:10.25 Stroke completed -- waiting for pressure to equalize ... @21:16:13.45 Intake.select :close @21:16:13.61 SSV.dial :exhaust @21:16:14.34 Exhaust.select :open @21:16:14.45 SS.reconfigure SSexhale @21:16:14.50 SS.seek 0ml @21:16:23.40 Exhaust.select :close @21:16:23.51 SSV.dial CBV @21:16:23.54
CBV.dial SSV @21:16:24.29 Allowing 130s for 25.0ml of remaining 1350.0ml @21:16:24.30 Intake.select :open @21:16:26.48 Intake @32.5psia, vacuum from 20.0 to 24.0psi @21:16:26.49 SS.reconfigure SSinhale.with maxSpeed: 43 @21:16:26.54 SS.seek 25ml @21:17:07.86 Stroke completed -- waiting for pressure to equalize ... @21:17:11.76 Intake.select :close @21:17:11.92 SSV.dial :exhaust @21:17:12.68 Exhaust.select :open @21:17:12.80 SS.reconfigure SSexhale @21:17:12.84 SS.seek 0ml @21:17:21.74 Exhaust.select :close @21:17:21.85 SSV.dial CBV @21:17:21.88
CBV.dial SSV @21:17:22.65 Allowing 130s for 25.0ml of remaining 1325.0ml @21:17:22.66 Intake.select :open @21:17:24.83 Intake @32.2psia, vacuum from 20.0 to 24.0psi @21:17:24.85 SS.reconfigure SSinhale.with maxSpeed: 43 @21:17:24.90 SS.seek 25ml @21:18:06.21 Stroke completed -- waiting for pressure to equalize ... @21:18:09.92 Intake.select :close @21:18:10.07 SSV.dial :exhaust @21:18:10.79 Exhaust.select :open @21:18:10.91 SS.reconfigure SSexhale @21:18:10.95 SS.seek 0ml @21:18:19.65 Exhaust.select :close @21:18:19.77 SSV.dial CBV @21:18:19.79
CBV.dial SSV @21:18:20.54 Allowing 130s for 25.0ml of remaining 1300.0ml @21:18:20.55 Intake.select :open @21:18:22.73 Intake @32.5psia, vacuum from 20.0 to 24.0psi @21:18:22.74 SS.reconfigure SSinhale.with maxSpeed: 43 @21:18:22.79 SS.seek 25ml @21:18:26.69 GoodNews.email "At 20:48:26 began in tube 2: mbaPhase\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:121\n{1 => 6, 2 => 16}\nCan@20:48:26, 16.6C, 79% humidity, 14.6psia, 15.186V, 0.809A, 0.809A avg, 12.28W",Subject: "Started TotaldeploymentFINAL3 phase mbaPhase" @21:18:26.72 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @21:18:26.74 Retry #4 of 10 after 10:00 @21:19:04.37
Stroke completed -- waiting for pressure to equalize ... @21:19:08.23 Intake.select :close @21:19:08.38 SSV.dial :exhaust @21:19:09.10 Exhaust.select :open @21:19:09.22 SS.reconfigure SSexhale @21:19:09.26 SS.seek 0ml @21:19:18.04 Exhaust.select :close @21:19:18.16 SSV.dial CBV @21:19:18.18
CBV.dial SSV @21:19:18.93 Allowing 130s for 25.0ml of remaining 1275.0ml @21:19:18.94 Intake.select :open @21:19:21.12 Intake @32.6psia, vacuum from 20.0 to 24.0psi @21:19:21.13 SS.reconfigure SSinhale.with maxSpeed: 43 @21:19:21.18 SS.seek 25ml @21:20:00.70 Vacuum too high -- waiting up to 40s @21:20:01.79 Intake @31.9psia, vacuum from 20.0 to 24.0psi @21:20:01.81 SS.reconfigure SSinhale.with maxSpeed: 39 @21:20:01.86 SS.seek 25ml @21:20:04.10 Stroke completed -- waiting for pressure to equalize ... @21:20:06.70 Intake.select :close @21:20:06.86 SSV.dial :exhaust @21:20:07.59 Exhaust.select :open @21:20:07.71 SS.reconfigure SSexhale @21:20:07.75 SS.seek 0ml @21:20:16.48 Exhaust.select :close @21:20:16.60 SSV.dial CBV @21:20:16.62
CBV.dial SSV @21:20:17.37 Allowing 130s for 25.0ml of remaining 1250.0ml @21:20:17.38 Intake.select :open @21:20:19.56 Intake @32.5psia, vacuum from 20.0 to 24.0psi @21:20:19.57 SS.reconfigure SSinhale.with maxSpeed: 39 @21:20:19.62 SS.seek 25ml @21:21:05.26 Stroke completed -- waiting for pressure to equalize ... @21:21:08.47 Intake.select :close @21:21:08.63 SSV.dial :exhaust @21:21:09.38 Exhaust.select :open @21:21:09.50 SS.reconfigure SSexhale @21:21:09.54 SS.seek 0ml @21:21:18.42 Exhaust.select :close @21:21:18.53 SSV.dial CBV @21:21:18.55
CBV.dial SSV @21:21:19.80 Allowing 130s for 25.0ml of remaining 1225.0ml @21:21:19.82 Intake.select :open @21:21:22.00 Intake @32.5psia, vacuum from 20.0 to 24.0psi @21:21:22.01 SS.reconfigure SSinhale.with maxSpeed: 39 @21:21:22.06 SS.seek 25ml @21:22:07.49 Stroke completed -- waiting for pressure to equalize ... @21:22:11.36 Intake.select :close @21:22:11.52 SSV.dial :exhaust @21:22:12.24 Exhaust.select :open @21:22:12.36 SS.reconfigure SSexhale @21:22:12.40 SS.seek 0ml @21:22:21.15 Exhaust.select :close @21:22:21.27 SSV.dial CBV @21:22:21.29
CBV.dial SSV @21:22:22.04 Allowing 130s for 25.0ml of remaining 1200.0ml @21:22:22.05 Intake.select :open @21:22:24.22 Intake @32.5psia, vacuum from 20.0 to 24.0psi @21:22:24.24 SS.reconfigure SSinhale.with maxSpeed: 39 @21:22:24.29 SS.seek 25ml @21:23:09.72 Stroke completed -- waiting for pressure to equalize ... @21:23:13.58 Intake.select :close @21:23:13.74 SSV.dial :exhaust @21:23:14.46 Exhaust.select :open @21:23:14.58 SS.reconfigure SSexhale @21:23:14.62 SS.seek 0ml @21:23:23.43 Exhaust.select :close @21:23:23.55 SSV.dial CBV @21:23:23.57
CBV.dial SSV @21:23:24.32 Allowing 130s for 25.0ml of remaining 1175.0ml @21:23:24.33 Intake.select :open @21:23:26.51 Intake @32.5psia, vacuum from 20.0 to 24.0psi @21:23:26.52 SS.reconfigure SSinhale.with maxSpeed: 39 @21:23:26.57 SS.seek 25ml @21:24:11.97 Stroke completed -- waiting for pressure to equalize ... @21:24:15.83 Intake.select :close @21:24:15.99 SSV.dial :exhaust @21:24:16.71 Exhaust.select :open @21:24:16.82 SS.reconfigure SSexhale @21:24:16.87 SS.seek 0ml @21:24:25.79 Exhaust.select :close @21:24:25.90 SSV.dial CBV @21:24:25.93
CBV.dial SSV @21:24:26.68 Allowing 130s for 25.0ml of remaining 1150.0ml @21:24:26.69 Intake.select :open @21:24:28.87 Intake @32.5psia, vacuum from 20.0 to 24.0psi @21:24:28.88 SS.reconfigure SSinhale.with maxSpeed: 39 @21:24:28.93 SS.seek 25ml @21:25:14.22 Stroke completed -- waiting for pressure to equalize ... @21:25:18.04 Intake.select :close @21:25:18.20 SSV.dial :exhaust @21:25:18.93 Exhaust.select :open @21:25:19.05 SS.reconfigure SSexhale @21:25:19.09 SS.seek 0ml @21:25:27.95 Exhaust.select :close @21:25:28.07 SSV.dial CBV @21:25:28.09
CBV.dial SSV @21:25:29.34 Allowing 130s for 25.0ml of remaining 1125.0ml @21:25:29.35 Intake.select :open @21:25:31.53 Intake @32.5psia, vacuum from 20.0 to 24.0psi @21:25:31.54 SS.reconfigure SSinhale.with maxSpeed: 39 @21:25:31.59 SS.seek 25ml @21:26:16.93 Stroke completed -- waiting for pressure to equalize ... @21:26:20.80 Intake.select :close @21:26:20.96 SSV.dial :exhaust @21:26:21.73 Exhaust.select :open @21:26:21.85 SS.reconfigure SSexhale @21:26:21.89 SS.seek 0ml @21:26:31.15 Exhaust.select :close @21:26:31.26 SSV.dial CBV @21:26:31.29
CBV.dial SSV @21:26:32.06 Allowing 130s for 25.0ml of remaining 1100.0ml @21:26:32.07 Intake.select :open @21:26:34.25 Intake @32.7psia, vacuum from 20.0 to 24.0psi @21:26:34.26 SS.reconfigure SSinhale.with maxSpeed: 39 @21:26:34.31 SS.seek 25ml @21:27:19.46 Stroke completed -- waiting for pressure to equalize ... @21:27:23.31 Intake.select :close @21:27:23.47 SSV.dial :exhaust @21:27:24.21 Exhaust.select :open @21:27:24.32 SS.reconfigure SSexhale @21:27:24.37 SS.seek 0ml @21:27:33.26 Exhaust.select :close @21:27:33.38 SSV.dial CBV @21:27:33.40
CBV.dial SSV @21:27:34.16 Allowing 130s for 25.0ml of remaining 1075.0ml @21:27:34.17 Intake.select :open @21:27:36.35 Intake @32.7psia, vacuum from 20.0 to 24.0psi @21:27:36.36 SS.reconfigure SSinhale.with maxSpeed: 39 @21:27:36.41 SS.seek 25ml @21:28:07.60 Vacuum too high -- waiting up to 40s @21:28:08.68 Intake @32.2psia, vacuum from 20.0 to 24.0psi @21:28:08.70 SS.reconfigure SSinhale.with maxSpeed: 35 @21:28:08.75 SS.seek 25ml @21:28:24.64 Stroke completed -- waiting for pressure to equalize ... @21:28:26.76 GoodNews.email "At 20:48:26 began in tube 2: mbaPhase\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:121\n{1 => 6, 2 => 16}\nCan@20:48:26, 16.6C, 79% humidity, 14.6psia, 15.186V, 0.809A, 0.809A avg, 12.28W",Subject: "Started TotaldeploymentFINAL3 phase mbaPhase" @21:28:26.79 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @21:28:26.80 Retry #5 of 10 after 10:00 @21:28:28.49
Intake.select :close @21:28:28.65 SSV.dial :exhaust @21:28:29.38 Exhaust.select :open @21:28:29.49 SS.reconfigure SSexhale @21:28:29.54 SS.seek 0ml @21:28:38.55 Exhaust.select :close @21:28:38.66 SSV.dial CBV @21:28:38.68
CBV.dial SSV @21:28:39.45 Allowing 130s for 25.0ml of remaining 1050.0ml @21:28:39.46 Intake.select :open @21:28:41.63 Intake @32.6psia, vacuum from 20.0 to 24.0psi @21:28:41.65 SS.reconfigure SSinhale.with maxSpeed: 35 @21:28:41.70 SS.seek 25ml @21:29:32.12 Stroke completed -- waiting for pressure to equalize ... @21:29:35.82 Intake.select :close @21:29:35.98 SSV.dial :exhaust @21:29:36.71 Exhaust.select :open @21:29:36.83 SS.reconfigure SSexhale @21:29:36.87 SS.seek 0ml @21:29:45.98 Exhaust.select :close @21:29:46.10 SSV.dial CBV @21:29:46.12
CBV.dial SSV @21:29:46.88 Allowing 130s for 25.0ml of remaining 1025.0ml @21:29:46.89 Intake.select :open @21:29:49.07 Intake @32.7psia, vacuum from 20.0 to 24.0psi @21:29:49.08 SS.reconfigure SSinhale.with maxSpeed: 35 @21:29:49.13 SS.seek 25ml @21:30:39.58 Stroke completed -- waiting for pressure to equalize ... @21:30:43.43 Intake.select :close @21:30:43.59 SSV.dial :exhaust @21:30:44.35 Exhaust.select :open @21:30:44.47 SS.reconfigure SSexhale @21:30:44.51 SS.seek 0ml @21:30:53.48 Exhaust.select :close @21:30:53.60 SSV.dial CBV @21:30:53.62
CBV.dial SSV @21:30:54.40 Allowing 130s for 25.0ml of remaining 1000.0ml @21:30:54.41 Intake.select :open @21:30:56.59 Intake @32.6psia, vacuum from 20.0 to 24.0psi @21:30:56.60 SS.reconfigure SSinhale.with maxSpeed: 35 @21:30:56.65 SS.seek 25ml @21:31:47.01 Stroke completed -- waiting for pressure to equalize ... @21:31:50.85 Intake.select :close @21:31:51.01 SSV.dial :exhaust @21:31:51.76 Exhaust.select :open @21:31:51.88 SS.reconfigure SSexhale @21:31:51.92 SS.seek 0ml @21:32:00.91 Exhaust.select :close @21:32:01.02 SSV.dial CBV @21:32:01.04
CBV.dial SSV @21:32:01.82 Allowing 130s for 25.0ml of remaining 975.0ml @21:32:01.83 Intake.select :open @21:32:04.00 Intake @32.8psia, vacuum from 20.0 to 24.0psi @21:32:04.02 SS.reconfigure SSinhale.with maxSpeed: 35 @21:32:04.07 SS.seek 25ml @21:32:54.39 Stroke completed -- waiting for pressure to equalize ... @21:32:58.85 Intake.select :close @21:32:59.01 SSV.dial :exhaust @21:32:59.74 Exhaust.select :open @21:32:59.85 SS.reconfigure SSexhale @21:32:59.90 SS.seek 0ml @21:33:08.77 Exhaust.select :close @21:33:08.88 SSV.dial CBV @21:33:08.91
CBV.dial SSV @21:33:09.66 Allowing 130s for 25.0ml of remaining 950.0ml @21:33:09.67 Intake.select :open @21:33:11.84 Intake @32.7psia, vacuum from 20.0 to 24.0psi @21:33:11.86 SS.reconfigure SSinhale.with maxSpeed: 35 @21:33:11.91 SS.seek 25ml @21:34:02.23 Stroke completed -- waiting for pressure to equalize ... @21:34:06.57 Intake.select :close @21:34:06.73 SSV.dial :exhaust @21:34:07.47 Exhaust.select :open @21:34:07.59 SS.reconfigure SSexhale @21:34:07.63 SS.seek 0ml @21:34:16.63 Exhaust.select :close @21:34:16.75 SSV.dial CBV @21:34:16.77
CBV.dial SSV @21:34:17.54 Allowing 130s for 25.0ml of remaining 925.0ml @21:34:17.55 Intake.select :open @21:34:19.74 Intake @32.7psia, vacuum from 20.0 to 24.0psi @21:34:19.76 SS.reconfigure SSinhale.with maxSpeed: 35 @21:34:19.81 SS.seek 25ml @21:35:10.30 Stroke completed -- waiting for pressure to equalize ... @21:35:14.79 Intake.select :close @21:35:14.95 SSV.dial :exhaust @21:35:15.69 Exhaust.select :open @21:35:15.81 SS.reconfigure SSexhale @21:35:15.85 SS.seek 0ml @21:35:24.91 Exhaust.select :close @21:35:25.03 SSV.dial CBV @21:35:25.05
CBV.dial SSV @21:35:25.82 Allowing 130s for 25.0ml of remaining 900.0ml @21:35:25.83 Intake.select :open @21:35:28.00 Intake @32.7psia, vacuum from 20.0 to 24.0psi @21:35:28.02 SS.reconfigure SSinhale.with maxSpeed: 35 @21:35:28.07 SS.seek 25ml @21:36:18.37 Stroke completed -- waiting for pressure to equalize ... @21:36:22.84 Intake.select :close @21:36:23.00 SSV.dial :exhaust @21:36:23.76 Exhaust.select :open @21:36:23.92 SS.reconfigure SSexhale @21:36:23.96 SS.seek 0ml @21:36:32.62 Exhaust.select :close @21:36:32.74 SSV.dial CBV @21:36:32.76
CBV.dial SSV @21:36:33.54 Allowing 130s for 25.0ml of remaining 875.0ml @21:36:33.55 Intake.select :open @21:36:35.72 Intake @32.7psia, vacuum from 20.0 to 24.0psi @21:36:35.74 SS.reconfigure SSinhale.with maxSpeed: 35 @21:36:35.79 SS.seek 25ml @21:37:22.72 Vacuum too high -- waiting up to 40s @21:37:23.80 Intake @32.2psia, vacuum from 20.0 to 24.0psi @21:37:23.82 SS.reconfigure SSinhale.with maxSpeed: 32 @21:37:23.87 SS.seek 25ml @21:37:28.13 Stroke completed -- waiting for pressure to equalize ... @21:37:31.98 Intake.select :close @21:37:32.14 SSV.dial :exhaust @21:37:33.37 Exhaust.select :open @21:37:33.49 SS.reconfigure SSexhale @21:37:33.53 SS.seek 0ml @21:37:42.37 Exhaust.select :close @21:37:42.49 SSV.dial CBV @21:37:42.51
CBV.dial SSV @21:37:43.29 Allowing 130s for 25.0ml of remaining 850.0ml @21:37:43.30 Intake.select :open @21:37:45.48 Intake @32.8psia, vacuum from 20.0 to 24.0psi @21:37:45.49 SS.reconfigure SSinhale.with maxSpeed: 32 @21:37:45.55 SS.seek 25ml @21:38:26.84 GoodNews.email "At 20:48:26 began in tube 2: mbaPhase\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:121\n{1 => 6, 2 => 16}\nCan@20:48:26, 16.6C, 79% humidity, 14.6psia, 15.186V, 0.809A, 0.809A avg, 12.28W",Subject: "Started TotaldeploymentFINAL3 phase mbaPhase" @21:38:26.87 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @21:38:26.89 Retry #6 of 10 after 10:00 @21:38:40.63
Stroke completed -- waiting for pressure to equalize ... @21:38:45.12 Intake.select :close @21:38:45.28 SSV.dial :exhaust @21:38:46.03 Exhaust.select :open @21:38:46.15 SS.reconfigure SSexhale @21:38:46.19 SS.seek 0ml @21:38:55.21 Exhaust.select :close @21:38:55.33 SSV.dial CBV @21:38:55.35
CBV.dial SSV @21:38:56.12 Allowing 130s for 25.0ml of remaining 825.0ml @21:38:56.13 Intake.select :open @21:38:58.31 Intake @32.8psia, vacuum from 20.0 to 24.0psi @21:38:58.32 SS.reconfigure SSinhale.with maxSpeed: 32 @21:38:58.37 SS.seek 25ml @21:39:53.07 Stroke completed -- waiting for pressure to equalize ... @21:39:57.55 Intake.select :close @21:39:57.70 SSV.dial :exhaust @21:39:58.44 Exhaust.select :open @21:39:58.56 SS.reconfigure SSexhale @21:39:58.60 SS.seek 0ml @21:40:07.54 Exhaust.select :close @21:40:07.66 SSV.dial CBV @21:40:07.68
CBV.dial SSV @21:40:08.46 Allowing 130s for 25.0ml of remaining 800.0ml @21:40:08.47 Intake.select :open @21:40:10.65 Intake @32.9psia, vacuum from 20.0 to 24.0psi @21:40:10.66 SS.reconfigure SSinhale.with maxSpeed: 32 @21:40:10.71 SS.seek 25ml @21:41:05.76 Stroke completed -- waiting for pressure to equalize ... @21:41:10.20 Intake.select :close @21:41:10.42 SSV.dial :exhaust @21:41:11.16 Exhaust.select :open @21:41:11.28 SS.reconfigure SSexhale @21:41:11.32 SS.seek 0ml @21:41:20.22 Exhaust.select :close @21:41:20.34 SSV.dial CBV @21:41:20.36
CBV.dial SSV @21:41:21.12 Allowing 130s for 25.0ml of remaining 775.0ml @21:41:21.13 Intake.select :open @21:41:23.30 Intake @32.7psia, vacuum from 20.0 to 24.0psi @21:41:23.32 SS.reconfigure SSinhale.with maxSpeed: 32 @21:41:23.37 SS.seek 25ml @21:42:18.46 Stroke completed -- waiting for pressure to equalize ... @21:42:23.40 Intake.select :close @21:42:23.57 SSV.dial :exhaust @21:42:24.30 Exhaust.select :open @21:42:24.42 SS.reconfigure SSexhale @21:42:24.46 SS.seek 0ml @21:42:33.40 Exhaust.select :close @21:42:33.52 SSV.dial CBV @21:42:33.54
CBV.dial SSV @21:42:34.30 Allowing 130s for 25.0ml of remaining 750.0ml @21:42:34.31 Intake.select :open @21:42:36.48 Intake @32.8psia, vacuum from 20.0 to 24.0psi @21:42:36.50 SS.reconfigure SSinhale.with maxSpeed: 32 @21:42:36.55 SS.seek 25ml @21:43:31.41 Stroke completed -- waiting for pressure to equalize ... @21:43:36.59 Intake.select :close @21:43:36.75 SSV.dial :exhaust @21:43:37.47 Exhaust.select :open @21:43:37.58 SS.reconfigure SSexhale @21:43:37.63 SS.seek 0ml @21:43:46.54 Exhaust.select :close @21:43:46.65 SSV.dial CBV @21:43:46.68
CBV.dial SSV @21:43:47.46 Allowing 130s for 25.0ml of remaining 725.0ml @21:43:47.47 Intake.select :open @21:43:49.64 Intake @32.8psia, vacuum from 20.0 to 24.0psi @21:43:49.66 SS.reconfigure SSinhale.with maxSpeed: 32 @21:43:49.71 SS.seek 25ml @21:44:44.90 Stroke completed -- waiting for pressure to equalize ... @21:44:49.98 Intake.select :close @21:44:50.14 SSV.dial :exhaust @21:44:50.88 Exhaust.select :open @21:44:51.00 SS.reconfigure SSexhale @21:44:51.04 SS.seek 0ml @21:44:59.99 Exhaust.select :close @21:45:00.11 SSV.dial CBV @21:45:00.13
CBV.dial SSV @21:45:00.90 Allowing 130s for 25.0ml of remaining 700.0ml @21:45:00.91 Intake.select :open @21:45:03.08 Intake @32.9psia, vacuum from 20.0 to 24.0psi @21:45:03.10 SS.reconfigure SSinhale.with maxSpeed: 32 @21:45:03.15 SS.seek 25ml @21:45:47.56 Vacuum too high -- waiting up to 40s @21:45:48.65 Intake @32.4psia, vacuum from 20.0 to 24.0psi @21:45:48.66 SS.reconfigure SSinhale.with maxSpeed: 29 @21:45:48.71 SS.seek 25ml @21:46:00.73 Stroke completed -- waiting for pressure to equalize ... @21:46:05.68 Intake.select :close @21:46:05.84 SSV.dial :exhaust @21:46:06.57 Exhaust.select :open @21:46:06.69 SS.reconfigure SSexhale @21:46:06.73 SS.seek 0ml @21:46:15.72 Exhaust.select :close @21:46:15.84 SSV.dial CBV @21:46:15.86
CBV.dial SSV @21:46:16.61 Allowing 130s for 25.0ml of remaining 675.0ml @21:46:16.62 Intake.select :open @21:46:18.80 Intake @32.8psia, vacuum from 20.0 to 24.0psi @21:46:18.82 SS.reconfigure SSinhale.with maxSpeed: 29 @21:46:18.87 SS.seek 25ml @21:47:19.13 Stroke completed -- waiting for pressure to equalize ... @21:47:23.62 Intake.select :close @21:47:23.77 SSV.dial :exhaust @21:47:24.51 Exhaust.select :open @21:47:24.63 SS.reconfigure SSexhale @21:47:24.67 SS.seek 0ml @21:47:33.55 Exhaust.select :close @21:47:33.67 SSV.dial CBV @21:47:33.69
CBV.dial SSV @21:47:34.44 Allowing 130s for 25.0ml of remaining 650.0ml @21:47:34.45 Intake.select :open @21:47:36.63 Intake @32.8psia, vacuum from 20.0 to 24.0psi @21:47:36.64 SS.reconfigure SSinhale.with maxSpeed: 29 @21:47:36.69 SS.seek 25ml @21:48:26.92 GoodNews.email "At 20:48:26 began in tube 2: mbaPhase\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:121\n{1 => 6, 2 => 16}\nCan@20:48:26, 16.6C, 79% humidity, 14.6psia, 15.186V, 0.809A, 0.809A avg, 12.28W",Subject: "Started TotaldeploymentFINAL3 phase mbaPhase" @21:48:26.95 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @21:48:26.97 Retry #7 of 10 after 10:00 @21:48:37.29
Stroke completed -- waiting for pressure to equalize ... @21:48:42.40 Intake.select :close @21:48:42.56 SSV.dial :exhaust @21:48:43.29 Exhaust.select :open @21:48:43.40 SS.reconfigure SSexhale @21:48:43.45 SS.seek 0ml @21:48:52.54 Exhaust.select :close @21:48:52.66 SSV.dial CBV @21:48:52.68
CBV.dial SSV @21:48:53.46 Allowing 130s for 25.0ml of remaining 625.0ml @21:48:53.47 Intake.select :open @21:48:55.64 Intake @32.7psia, vacuum from 20.0 to 24.0psi @21:48:55.66 SS.reconfigure SSinhale.with maxSpeed: 29 @21:48:55.71 SS.seek 25ml @21:49:56.17 Stroke completed -- waiting for pressure to equalize ... @21:50:01.37 Intake.select :close @21:50:01.52 SSV.dial :exhaust @21:50:02.25 Exhaust.select :open @21:50:02.36 SS.reconfigure SSexhale @21:50:02.41 SS.seek 0ml @21:50:11.27 Exhaust.select :close @21:50:11.38 SSV.dial CBV @21:50:11.41
CBV.dial SSV @21:50:12.18 Allowing 130s for 25.0ml of remaining 600.0ml @21:50:12.19 Intake.select :open @21:50:14.37 Intake @32.9psia, vacuum from 20.0 to 24.0psi @21:50:14.79 SS.reconfigure SSinhale.with maxSpeed: 29 @21:50:14.92 SS.seek 25ml @21:51:15.60 Stroke completed -- waiting for pressure to equalize ... @21:51:20.81 Intake.select :close @21:51:20.97 SSV.dial :exhaust @21:51:21.71 Exhaust.select :open @21:51:21.83 SS.reconfigure SSexhale @21:51:21.87 SS.seek 0ml @21:51:31.02 Exhaust.select :close @21:51:31.14 SSV.dial CBV @21:51:31.16
CBV.dial SSV @21:51:31.93 Allowing 130s for 25.0ml of remaining 575.0ml @21:51:31.94 Intake.select :open @21:51:34.11 Intake @32.9psia, vacuum from 20.0 to 24.0psi @21:51:34.13 SS.reconfigure SSinhale.with maxSpeed: 29 @21:51:34.18 SS.seek 25ml @21:52:34.89 Stroke completed -- waiting for pressure to equalize ... @21:52:40.01 Intake.select :close @21:52:40.17 SSV.dial :exhaust @21:52:40.89 Exhaust.select :open @21:52:41.01 SS.reconfigure SSexhale @21:52:41.05 SS.seek 0ml @21:52:50.00 Exhaust.select :close @21:52:50.12 SSV.dial CBV @21:52:50.14
CBV.dial SSV @21:52:50.89 Allowing 130s for 25.0ml of remaining 549.9ml @21:52:50.90 Intake.select :open @21:52:53.07 Intake @32.7psia, vacuum from 20.0 to 24.0psi @21:52:53.09 SS.reconfigure SSinhale.with maxSpeed: 29 @21:52:53.14 SS.seek 25ml @21:53:53.55 Stroke completed -- waiting for pressure to equalize ... @21:53:59.27 Intake.select :close @21:53:59.43 SSV.dial :exhaust @21:54:00.16 Exhaust.select :open @21:54:00.28 SS.reconfigure SSexhale @21:54:00.32 SS.seek 0ml @21:54:09.24 Exhaust.select :close @21:54:09.35 SSV.dial CBV @21:54:09.38
CBV.dial SSV @21:54:10.14 Allowing 130s for 25.0ml of remaining 524.9ml @21:54:10.15 Intake.select :open @21:54:12.33 Intake @32.9psia, vacuum from 20.0 to 24.0psi @21:54:12.34 SS.reconfigure SSinhale.with maxSpeed: 29 @21:54:12.39 SS.seek 25ml @21:55:13.02 Stroke completed -- waiting for pressure to equalize ... @21:55:18.61 Intake.select :close @21:55:18.77 SSV.dial :exhaust @21:55:19.52 Exhaust.select :open @21:55:19.64 SS.reconfigure SSexhale @21:55:19.68 SS.seek 0ml @21:55:28.61 Exhaust.select :close @21:55:28.73 SSV.dial CBV @21:55:28.75
CBV.dial SSV @21:55:29.52 Allowing 130s for 25.0ml of remaining 499.9ml @21:55:29.53 Intake.select :open @21:55:31.71 Intake @32.7psia, vacuum from 20.0 to 24.0psi @21:55:31.73 SS.reconfigure SSinhale.with maxSpeed: 29 @21:55:31.78 SS.seek 25ml @21:56:27.97 Vacuum too high -- waiting up to 40s @21:56:29.30 Intake @32.6psia, vacuum from 20.0 to 24.0psi @21:56:29.32 SS.reconfigure SSinhale.with maxSpeed: 26 @21:56:29.37 SS.seek 25ml @21:56:34.58 Stroke completed -- waiting for pressure to equalize ... @21:56:39.69 Intake.select :close @21:56:39.87 SSV.dial :exhaust @21:56:40.61 Exhaust.select :open @21:56:40.73 SS.reconfigure SSexhale @21:56:40.77 SS.seek 0ml @21:56:49.69 Exhaust.select :close @21:56:49.81 SSV.dial CBV @21:56:49.83
CBV.dial SSV @21:56:50.60 Allowing 130s for 25.0ml of remaining 474.9ml @21:56:50.61 Intake.select :open @21:56:52.78 Intake @32.9psia, vacuum from 20.0 to 24.0psi @21:56:52.80 SS.reconfigure SSinhale.with maxSpeed: 26 @21:56:52.85 SS.seek 25ml @21:58:00.38 Stroke completed -- waiting for pressure to equalize ... @21:58:06.10 Intake.select :close @21:58:06.26 SSV.dial :exhaust @21:58:06.99 Exhaust.select :open @21:58:07.10 SS.reconfigure SSexhale @21:58:07.18 SS.seek 0ml @21:58:15.94 Exhaust.select :close @21:58:16.06 SSV.dial CBV @21:58:16.08
CBV.dial SSV @21:58:16.85 Allowing 130s for 25.0ml of remaining 449.9ml @21:58:16.86 Intake.select :open @21:58:19.03 Intake @32.8psia, vacuum from 20.0 to 24.0psi @21:58:19.05 SS.reconfigure SSinhale.with maxSpeed: 26 @21:58:19.10 SS.seek 25ml @21:58:26.99 GoodNews.email "At 20:48:26 began in tube 2: mbaPhase\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:121\n{1 => 6, 2 => 16}\nCan@20:48:26, 16.6C, 79% humidity, 14.6psia, 15.186V, 0.809A, 0.809A avg, 12.28W",Subject: "Started TotaldeploymentFINAL3 phase mbaPhase" @21:58:27.02 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @21:58:27.03 Retry #8 of 10 after 10:00 @21:59:26.88
Stroke completed -- waiting for pressure to equalize ... @21:59:32.45 Intake.select :close @21:59:32.61 SSV.dial :exhaust @21:59:33.36 Exhaust.select :open @21:59:33.48 SS.reconfigure SSexhale @21:59:33.52 SS.seek 0ml @21:59:42.44 Exhaust.select :close @21:59:42.56 SSV.dial CBV @21:59:42.58
CBV.dial SSV @21:59:43.35 Allowing 130s for 25.0ml of remaining 424.9ml @21:59:43.36 Intake.select :open @21:59:45.54 Intake @32.6psia, vacuum from 20.0 to 24.0psi @21:59:45.55 SS.reconfigure SSinhale.with maxSpeed: 26 @21:59:45.60 SS.seek 25ml @22:00:53.00 Stroke completed -- waiting for pressure to equalize ... @22:00:58.75 Intake.select :close @22:00:58.91 SSV.dial :exhaust @22:00:59.66 Exhaust.select :open @22:00:59.77 SS.reconfigure SSexhale @22:00:59.82 SS.seek 0ml @22:01:08.69 Exhaust.select :close @22:01:08.81 SSV.dial CBV @22:01:08.83
CBV.dial SSV @22:01:09.60 Allowing 130s for 25.0ml of remaining 399.9ml @22:01:09.61 Intake.select :open @22:01:11.79 Intake @32.8psia, vacuum from 20.0 to 24.0psi @22:01:11.80 SS.reconfigure SSinhale.with maxSpeed: 26 @22:01:11.85 SS.seek 25ml @22:02:19.40 Stroke completed -- waiting for pressure to equalize ... @22:02:26.43 Intake.select :close @22:02:26.59 SSV.dial :exhaust @22:02:27.31 Exhaust.select :open @22:02:27.42 SS.reconfigure SSexhale @22:02:27.47 SS.seek 0ml @22:02:36.36 Exhaust.select :close @22:02:36.47 SSV.dial CBV @22:02:36.50
CBV.dial SSV @22:02:37.25 Allowing 130s for 25.0ml of remaining 374.9ml @22:02:37.26 Intake.select :open @22:02:39.43 Intake @32.8psia, vacuum from 20.0 to 24.0psi @22:02:39.45 SS.reconfigure SSinhale.with maxSpeed: 26 @22:02:39.50 SS.seek 25ml @22:03:46.97 Stroke completed -- waiting for pressure to equalize ... @22:03:53.83 Intake.select :close @22:03:53.99 SSV.dial :exhaust @22:03:54.72 Exhaust.select :open @22:03:54.84 SS.reconfigure SSexhale @22:03:54.88 SS.seek 0ml @22:04:03.81 Exhaust.select :close @22:04:03.93 SSV.dial CBV @22:04:03.95
CBV.dial SSV @22:04:04.72 Allowing 130s for 25.0ml of remaining 349.9ml @22:04:04.73 Intake.select :open @22:04:06.90 Intake @32.7psia, vacuum from 20.0 to 24.0psi @22:04:06.92 SS.reconfigure SSinhale.with maxSpeed: 26 @22:04:06.97 SS.seek 25ml @22:05:14.44 Stroke completed -- waiting for pressure to equalize ... @22:05:21.48 Intake.select :close @22:05:21.64 SSV.dial :exhaust @22:05:22.38 Exhaust.select :open @22:05:22.50 SS.reconfigure SSexhale @22:05:22.54 SS.seek 0ml @22:05:31.14 Exhaust.select :close @22:05:31.26 SSV.dial CBV @22:05:31.28
CBV.dial SSV @22:05:32.05 Allowing 130s for 25.0ml of remaining 324.9ml @22:05:32.06 Intake.select :open @22:05:34.24 Intake @32.9psia, vacuum from 20.0 to 24.0psi @22:05:34.25 SS.reconfigure SSinhale.with maxSpeed: 26 @22:05:34.30 SS.seek 25ml @22:06:33.15 Vacuum too high -- waiting up to 40s @22:06:34.48 Intake @32.6psia, vacuum from 20.0 to 24.0psi @22:06:34.49 SS.reconfigure SSinhale.with maxSpeed: 23 @22:06:34.54 SS.seek 25ml @22:06:44.90 Stroke completed -- waiting for pressure to equalize ... @22:06:51.27 Intake.select :close @22:06:51.43 SSV.dial :exhaust @22:06:52.20 Exhaust.select :open @22:06:52.32 SS.reconfigure SSexhale @22:06:52.36 SS.seek 0ml @22:07:01.11 Exhaust.select :close @22:07:01.22 SSV.dial CBV @22:07:01.25
CBV.dial SSV @22:07:02.02 Allowing 130s for 25.0ml of remaining 299.9ml @22:07:02.03 Intake.select :open @22:07:04.20 Intake @32.8psia, vacuum from 20.0 to 24.0psi @22:07:04.22 SS.reconfigure SSinhale.with maxSpeed: 23 @22:07:04.27 SS.seek 25ml @22:08:20.77 Stroke completed -- waiting for pressure to equalize ... @22:08:27.06 GoodNews.email "At 20:48:26 began in tube 2: mbaPhase\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:121\n{1 => 6, 2 => 16}\nCan@20:48:26, 16.6C, 79% humidity, 14.6psia, 15.186V, 0.809A, 0.809A avg, 12.28W",Subject: "Started TotaldeploymentFINAL3 phase mbaPhase" @22:08:27.09 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @22:08:27.11 Retry #9 of 10 after 10:00 @22:08:27.65
Intake.select :close @22:08:27.81 SSV.dial :exhaust @22:08:28.55 Exhaust.select :open @22:08:28.67 SS.reconfigure SSexhale @22:08:28.71 SS.seek 0ml @22:08:37.64 Exhaust.select :close @22:08:37.76 SSV.dial CBV @22:08:37.78
CBV.dial SSV @22:08:38.56 Allowing 130s for 25.0ml of remaining 274.9ml @22:08:38.57 Intake.select :open @22:08:40.75 Intake @32.8psia, vacuum from 20.0 to 24.0psi @22:08:40.76 SS.reconfigure SSinhale.with maxSpeed: 23 @22:08:40.81 SS.seek 25ml @22:09:56.92 Stroke completed -- waiting for pressure to equalize ... @22:10:03.29 Intake.select :close @22:10:03.45 SSV.dial :exhaust @22:10:04.19 Exhaust.select :open @22:10:04.31 SS.reconfigure SSexhale @22:10:04.35 SS.seek 0ml @22:10:13.28 Exhaust.select :close @22:10:13.39 SSV.dial CBV @22:10:13.42
CBV.dial SSV @22:10:14.19 Allowing 130s for 25.0ml of remaining 249.9ml @22:10:14.20 Intake.select :open @22:10:16.38 Intake @32.7psia, vacuum from 20.0 to 24.0psi @22:10:16.39 SS.reconfigure SSinhale.with maxSpeed: 23 @22:10:16.45 SS.seek 25ml @22:11:32.74 Stroke completed -- waiting for pressure to equalize ... @22:11:40.33 Intake.select :close @22:11:40.49 SSV.dial :exhaust @22:11:41.23 Exhaust.select :open @22:11:41.34 SS.reconfigure SSexhale @22:11:41.39 SS.seek 0ml @22:11:50.31 Exhaust.select :close @22:11:50.42 SSV.dial CBV @22:11:50.45
CBV.dial SSV @22:11:51.22 Allowing 130s for 25.0ml of remaining 224.9ml @22:11:51.23 Intake.select :open @22:11:53.40 Intake @32.9psia, vacuum from 20.0 to 24.0psi @22:11:53.42 SS.reconfigure SSinhale.with maxSpeed: 23 @22:11:53.47 SS.seek 25ml @22:13:09.61 Stroke completed -- waiting for pressure to equalize ... @22:13:18.42 Intake.select :close @22:13:18.57 SSV.dial :exhaust @22:13:19.31 Exhaust.select :open @22:13:19.43 SS.reconfigure SSexhale @22:13:19.47 SS.seek 0ml @22:13:28.33 Exhaust.select :close @22:13:28.45 SSV.dial CBV @22:13:28.47
CBV.dial SSV @22:13:29.25 Allowing 130s for 25.0ml of remaining 199.9ml @22:13:29.26 Intake.select :open @22:13:31.44 Intake @32.9psia, vacuum from 20.0 to 24.0psi @22:13:31.45 SS.reconfigure SSinhale.with maxSpeed: 23 @22:13:31.50 SS.seek 25ml @22:14:47.69 Stroke completed -- waiting for pressure to equalize ... @22:14:57.19 Intake.select :close @22:14:57.35 SSV.dial :exhaust @22:14:58.10 Exhaust.select :open @22:14:58.22 SS.reconfigure SSexhale @22:14:58.26 SS.seek 0ml @22:15:07.23 Exhaust.select :close @22:15:07.35 SSV.dial CBV @22:15:07.37
CBV.dial SSV @22:15:08.14 Allowing 130s for 25.0ml of remaining 174.9ml @22:15:08.15 Intake.select :open @22:15:10.32 Intake @32.9psia, vacuum from 20.0 to 24.0psi @22:15:10.34 SS.reconfigure SSinhale.with maxSpeed: 23 @22:15:10.39 SS.seek 25ml @22:16:26.59 Stroke completed -- waiting for pressure to equalize ... @22:16:38.52 Intake.select :close @22:16:38.68 SSV.dial :exhaust @22:16:39.42 Exhaust.select :open @22:16:39.54 SS.reconfigure SSexhale @22:16:39.58 SS.seek 0ml @22:16:48.50 Exhaust.select :close @22:16:48.62 SSV.dial CBV @22:16:48.64
CBV.dial SSV @22:16:49.41 Allowing 130s for 25.0ml of remaining 149.9ml @22:16:49.42 Intake.select :open @22:16:51.60 Intake @32.8psia, vacuum from 20.0 to 24.0psi @22:16:51.61 SS.reconfigure SSinhale.with maxSpeed: 23 @22:16:51.67 SS.seek 25ml @22:18:07.83 Stroke completed -- waiting for pressure to equalize ... @22:18:20.52 Intake.select :close @22:18:20.68 SSV.dial :exhaust @22:18:21.42 Exhaust.select :open @22:18:21.53 SS.reconfigure SSexhale @22:18:21.58 SS.seek 0ml @22:18:27.13 GoodNews.email "At 20:48:26 began in tube 2: mbaPhase\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:121\n{1 => 6, 2 => 16}\nCan@20:48:26, 16.6C, 79% humidity, 14.6psia, 15.186V, 0.809A, 0.809A avg, 12.28W",Subject: "Started TotaldeploymentFINAL3 phase mbaPhase" @22:18:27.16 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @22:18:27.17 Retry #10 of 10 after 10:00 @22:18:30.64
Exhaust.select :close @22:18:30.76 SSV.dial CBV @22:18:30.78
CBV.dial SSV @22:18:31.55 Allowing 130s for 25.0ml of remaining 124.9ml @22:18:31.56 Intake.select :open @22:18:33.74 Intake @32.9psia, vacuum from 20.0 to 24.0psi @22:18:33.75 SS.reconfigure SSinhale.with maxSpeed: 23 @22:18:33.81 SS.seek 25ml @22:19:49.74 Stroke completed -- waiting for pressure to equalize ... @22:20:02.94 Intake.select :close @22:20:03.10 SSV.dial :exhaust @22:20:03.84 Exhaust.select :open @22:20:03.96 SS.reconfigure SSexhale @22:20:04.00 SS.seek 0ml @22:20:12.72 Exhaust.select :close @22:20:12.84 SSV.dial CBV @22:20:12.86
CBV.dial SSV @22:20:13.65 Allowing 130s for 25.0ml of remaining 99.9ml @22:20:13.66 Intake.select :open @22:20:15.83 Intake @32.9psia, vacuum from 20.0 to 24.0psi @22:20:15.85 SS.reconfigure SSinhale.with maxSpeed: 23 @22:20:15.90 SS.seek 25ml @22:21:32.34 Stroke completed -- waiting for pressure to equalize ... @22:21:46.91 Intake.select :close @22:21:47.07 SSV.dial :exhaust @22:21:47.81 Exhaust.select :open @22:21:47.93 SS.reconfigure SSexhale @22:21:47.97 SS.seek 0ml @22:21:56.87 Exhaust.select :close @22:21:56.99 SSV.dial CBV @22:21:57.01
CBV.dial SSV @22:21:57.78 Allowing 130s for 25.0ml of remaining 74.9ml @22:21:57.79 Intake.select :open @22:21:59.97 Intake @32.8psia, vacuum from 20.0 to 24.0psi @22:21:59.99 SS.reconfigure SSinhale.with maxSpeed: 23 @22:22:00.04 SS.seek 25ml @22:22:56.65 Vacuum too high -- waiting up to 40s @22:22:57.99 Intake @32.7psia, vacuum from 20.0 to 24.0psi @22:22:58.01 SS.reconfigure SSinhale.with maxSpeed: 21 @22:22:58.06 SS.seek 25ml @22:23:19.66 Stroke completed -- waiting for pressure to equalize ... @22:23:34.16 Intake.select :close @22:23:34.32 SSV.dial :exhaust @22:23:35.06 Exhaust.select :open @22:23:35.18 SS.reconfigure SSexhale @22:23:35.24 SS.seek 0ml @22:23:44.14 Exhaust.select :close @22:23:44.25 SSV.dial CBV @22:23:44.28
CBV.dial SSV @22:23:45.04 Allowing 130s for 25.0ml of remaining 49.9ml @22:23:45.05 Intake.select :open @22:23:47.23 Intake @32.9psia, vacuum from 20.0 to 24.0psi @22:23:47.24 SS.reconfigure SSinhale.with maxSpeed: 21 @22:23:47.29 SS.seek 25ml @22:25:10.63 Stroke completed -- waiting for pressure to equalize ... @22:25:25.24 Intake.select :close @22:25:25.40 SSV.dial :exhaust @22:25:26.15 Exhaust.select :open @22:25:26.27 SS.reconfigure SSexhale @22:25:26.31 SS.seek 0ml @22:25:35.36 Exhaust.select :close @22:25:35.47 SSV.dial CBV @22:25:35.50
CBV.dial SSV @22:25:36.28 Allowing 130s for 24.9ml of remaining 24.9ml @22:25:36.29 Intake.select :open @22:25:38.46 Intake @32.9psia, vacuum from 20.0 to 24.0psi @22:25:38.48 SS.reconfigure SSinhale.with maxSpeed: 21 @22:25:38.53 SS.seek 24.93ml @22:27:01.71 Stroke completed -- waiting for pressure to equalize ... @22:27:19.49 Intake.select :close @22:27:19.65 SSV.dial :exhaust @22:27:20.39 Exhaust.select :open @22:27:20.51 SS.reconfigure SSexhale @22:27:20.55 SS.seek 0ml @22:27:29.43 Exhaust.select :close @22:27:29.54 Sampled 2000.0ml @22:27:29.56 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @22:27:29.57 Intake.select :close @22:27:29.64 CSV.dial :waste @22:27:29.70 CS.seek 0ml @22:27:29.81 SSV.dial CBV,avoiding: :exhaust @22:27:29.84
CBV.dial SSV @22:27:29.90 CSV.dial :air @22:27:30.62 CS.seek 10ml @22:27:31.62
CTV.dial :air @22:27:32.35 delay 15 seconds @22:27:47.38 SSV.dial :spit,avoiding: :exhaust @22:27:48.65 CTV.dial CSV,avoiding: :intake @22:27:48.69
SS.seek 0ml @22:27:48.83 SSV.dial CBV,avoiding: :exhaust @22:27:52.66 CBV.dial between SSV and waste2 @22:27:53.06 CSV.dial between air and CTV @22:27:53.37 CS.seek 8.489ml @22:27:57.73 CTV.dial CSV,avoiding: :air @22:27:57.76
CSV.dial CTV @22:27:58.09 CS.reconfigure CSpressurize @22:27:58.14 CS.seek 0ml @22:28:19.83 BubblePoint: Intake @68.3psia, Outlet @12.6psia @22:28:19.84 CBV.dial SSV @22:28:20.18 SS.reconfigure SSconfig @22:28:20.24 SS.seek 10ml @22:28:25.81 delay 15 seconds @22:28:27.20 GoodNews.email "At 20:48:26 began in tube 2: mbaPhase\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:121\n{1 => 6, 2 => 16}\nCan@20:48:26, 16.6C, 79% humidity, 14.6psia, 15.186V, 0.809A, 0.809A avg, 12.28W",Subject: "Started TotaldeploymentFINAL3 phase mbaPhase" @22:28:27.23 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @22:28:27.24 BACfans.email "Started mfb in tube 2 at 20:52:30CEST10-Sep-19\n{1 => 6, 2 => 16}\nCan@20:48:26, 16.6C, 79% humidity, 14.6psia, 15.186V, 0.809A, 0.809A avg, 12.28W",Subject: "pcrBAC sampling at most 2000ml, wcr at most 2000ml" @22:28:27.27 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @22:28:27.28 Retry #1 of 10 after 10:00 @22:28:40.94
Evacuated: Intake @36.2psia, Outlet @-1.0psia @22:28:40.96 SSV.dial :spit,avoiding: :exhaust @22:28:42.70 SS.reconfigure SSexhale @22:28:42.83 SS.seek 0ml @22:28:48.09 SS.reconfigure SSfast @22:28:48.14 SS.seek 5ml @22:28:50.79 SSV.dial CBV,avoiding: :exhaust @22:28:50.81
CBV.dial SSV @22:28:52.08 CTV.dial :air,avoiding: :intake @22:28:53.34 CSV.dial CTV @22:28:53.38
SS.seek 10ml @22:28:54.38 CSV.dial :air @22:28:54.82 CS.reconfigure CSfast @22:28:54.87 CS.seek 0ml @22:28:55.95
SSV.dial :spit,avoiding: :exhaust @22:28:56.38 CS.coast @22:28:57.21
SS.reconfigure SSexhale @22:28:57.25 SS.seek 0ml @22:29:02.08 SS.reconfigure SSfast @22:29:02.13 SS.seek 5ml @22:29:04.77 SSV.dial CBV,avoiding: :exhaust @22:29:04.79
CBV.dial SSV @22:29:07.07 SSV.dial :spit,avoiding: :exhaust @22:29:08.36 SS.reconfigure SSconfig @22:29:08.41 SS.seek 0ml @22:29:11.53 SS.coast @22:29:11.57 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @22:29:11.58 Intake.select :close @22:29:11.66 CSV.dial :waste @22:29:11.79 SSV.dial CBV,avoiding: :exhaust @22:29:11.82
CBV.dial SSV @22:29:12.46 CS.seek 0ml @22:29:12.53 CSV.dial :air @22:29:13.08
CTV.dial :air @22:29:13.10 delay 15 seconds @22:29:13.25 CS.seek 10ml @22:29:28.12
SSV.dial :spit,avoiding: :exhaust @22:29:29.38 CTV.dial CSV,avoiding: :intake @22:29:29.42
SS.reconfigure SSexhale @22:29:29.53 SS.seek 0ml @22:29:29.58 SSV.dial CBV,avoiding: :exhaust @22:29:35.07 CBV.dial between SSV and waste2 @22:29:35.50 CSV.dial between air and CTV @22:29:35.81 CS.seek 7.876ml @22:29:41.35 CTV.dial CSV,avoiding: :air @22:29:41.38
CSV.dial CTV @22:29:41.70 CS.reconfigure CSpressurize @22:29:41.74 CS.seek 0ml @22:30:02.54 CSV.dial :air @22:30:02.99 CS.reconfigure CSfast @22:30:03.04 CS.seek 10ml @22:30:24.69 CSV.dial between air and CTV @22:30:25.03 CS.seek 3.061ml @22:30:40.19 CTV.dial CSV,avoiding: :air @22:30:40.22
CSV.dial CTV @22:30:40.55 CS.reconfigure CSpressurize @22:30:40.59 CS.seek 0ml @22:30:47.78 BubblePoint: Intake @68.0psia, Outlet @12.6psia @22:30:47.79 CBV.dial SSV @22:30:48.12 SS.reconfigure SSconfig @22:30:48.18 SS.seek 10ml @22:30:53.57 delay 15 seconds @22:31:09.20 Evacuated: Intake @37.4psia, Outlet @14.7psia @22:31:09.21 SSV.dial :spit,avoiding: :exhaust @22:31:10.48 SS.reconfigure SSexhale @22:31:10.52 SS.seek 0ml @22:31:15.35 SS.reconfigure SSfast @22:31:15.40 SS.seek 5ml @22:31:18.02 SSV.dial CBV,avoiding: :exhaust @22:31:18.04
CBV.dial SSV @22:31:19.31 CTV.dial :air,avoiding: :intake @22:31:20.59 CSV.dial CTV @22:31:20.63
SS.seek 10ml @22:31:21.63 CSV.dial :air @22:31:22.08 CS.reconfigure CSfast @22:31:22.13 CS.seek 0ml @22:31:23.25
SSV.dial :spit,avoiding: :exhaust @22:31:24.29 CS.coast @22:31:24.51
SS.reconfigure SSexhale @22:31:24.55 SS.seek 0ml @22:31:29.71 SS.reconfigure SSfast @22:31:29.76 SS.seek 5ml @22:31:32.37 SSV.dial CBV,avoiding: :exhaust @22:31:32.39
CBV.dial SSV @22:31:34.68 SSV.dial :spit,avoiding: :exhaust @22:31:35.92 SS.reconfigure SSconfig @22:31:35.97 SS.seek 0ml @22:31:38.95 SS.coast @22:31:38.99 Lysing: Adding 1.5ml lysis @22:31:39.00 CBV.dial between CSV and waste1,avoiding: :DBV @22:31:40.42 CTV.dial CSV @22:31:41.70 CSV.dial CSR @22:31:42.49 Pulling 1ml of CSR.lysis @22:31:42.50 CSR.select :lysis @22:31:42.63 CS.reconfigure CSconfig @22:31:42.67 CS.seek 1ml @22:31:46.26 delay 5 seconds @22:31:51.29 CSR.select :air @22:31:51.47 CS.seek 6ml @22:32:05.13 delay 2 seconds @22:32:07.15 CSV.dial :waste1 @22:32:08.37 CS.reconfigure CSfast @22:32:08.42 CS.seek :empty @22:32:21.82 CSV.dial CSR @22:32:23.10 Pulling 1.5ml of CSR.lysis @22:32:23.11 CSR.select :lysis @22:32:23.24 CS.reconfigure CSconfig @22:32:23.28 CS.seek 1.5ml @22:32:28.20 delay 5 seconds @22:32:33.23 CSR.select :air @22:32:33.41 CS.seek 4.5ml @22:32:41.98 delay 2 seconds @22:32:44.00 CSV.dial CTV @22:32:44.46 CS.seek 1.275ml @22:32:53.71 Heating sh1 puck from tube 2 in Collection Clamp to 85C for 5:00 @22:32:53.73 CH.seek 85C,8:00 @22:35:19.83 CH.hold 5:00,85C @22:38:27.31 BACfans.email "Started mfb in tube 2 at 20:52:30CEST10-Sep-19\n{1 => 6, 2 => 16}\nCan@20:48:26, 16.6C, 79% humidity, 14.6psia, 15.186V, 0.809A, 0.809A avg, 12.28W",Subject: "pcrBAC sampling at most 2000ml, wcr at most 2000ml" @22:38:27.34 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @22:38:27.36 Retry #2 of 10 after 10:00 @22:40:20.01
Collection Puck Heater at 84.9C after lysing @22:40:20.57 CS.seek 0ml @22:40:24.99 CTV.dial between spare and air,avoiding: :intake @22:40:25.97 CSV.dial :air @22:40:26.41 CS.seek 5ml @22:40:40.17 CSV.dial CTV @22:40:40.62 delay 2 seconds @22:40:42.65 CSV.dial :air @22:40:43.22 delay 2 seconds @22:40:45.25 CSV.dial CTV @22:40:45.69 delay 2 seconds @22:40:47.72 CSV.dial :air @22:40:48.17 delay 2 seconds @22:40:50.21 CS.seek 0ml @22:41:03.92 Recovering Lysate into Collection syringe @22:41:03.93 CSV.dial CBV @22:41:04.89 CBV.dial CSV @22:41:05.29 CS.seek 5ml @22:41:18.98 delay 5 seconds @22:41:24.01 CTV.dial :air @22:41:24.54 CS.seek 7ml @22:41:30.80 delay 5 seconds @22:41:35.83 CSV.dial :air2 @22:41:36.27 CS.seek 10ml @22:41:44.98 CSV.dial between air and CTV,avoiding: CBV @22:41:46.08 Lysing Complete @22:41:46.16 Storing sh1 puck in Collection Clamp to tube 1 @22:41:46.36 SC.reconfigure SCtight @22:41:46.41 SC.seek 1 @22:41:48.53 SC.reconfigure SCloose @22:41:48.76 Elbow.reconfigure TightElbow @22:41:48.86 Elbow.seek 1 @22:41:50.83 Forearm.reconfigure TightForearm @22:41:50.88 Forearm.seek 1 + 700 counts @22:41:51.93 SE.reconfigure SEpick @22:41:52.02 SE.seek beamBreak @22:41:54.54 SE.configure SEconfig @22:41:54.71 Elbow.reconfigure LimpElbow @22:41:54.76 Forearm.reconfigure LooseForearm @22:41:54.80 Forearm.seek 1 @22:41:55.12 Elbow.hold @22:41:55.19 Elbow.reconfigure TightElbow @22:41:55.30 SE.seek 5.08 Pucks below Top Plate @22:41:55.81 Forearm.force -20 @22:41:55.85 Forearm.seek 1 + 310 counts @22:41:56.19 Forearm.reconfigure TightForearm @22:41:56.24 Forearm.seek 1 + 700 counts @22:41:56.59 SE.seek 6.08 Pucks below Top Plate @22:41:57.22 Forearm.reconfigure LooseForearm @22:41:57.27 Forearm.seek :retract @22:41:58.17 Elbow.seek CC @22:41:59.84 Elbow.reconfigure LooseElbow @22:41:59.94 Forearm.seek CC @22:42:01.02 Hand.close @22:42:02.09 CC.seek :open @22:42:06.58 Forearm.seek :retract @22:42:07.62 Elbow.reconfigure TightElbow @22:42:07.67 Elbow.seek 1 @22:42:09.59 Elbow.reconfigure LimpElbow @22:42:09.64 Forearm.seek 1 @22:42:10.67 Elbow.hold @22:42:10.74 Elbow.reconfigure TightElbow @22:42:10.79 Hand.open @22:42:11.61 Forearm.force -20 @22:42:11.66 Forearm.seek 1 + 347 counts @22:42:11.99 Forearm.reconfigure TightForearm @22:42:12.03 Forearm.seek 1 + 700 counts @22:42:12.39 SE.seek :down @22:42:13.55
Forearm.reconfigure LooseForearm @22:42:13.60 Forearm.seek :retract @22:42:14.61 SE.coast @22:42:14.66
SC.coast @22:42:14.70 Tranferring lysate to PRV @22:42:14.71 CSV.dial :air @22:42:15.02 CS.seek 10ml @22:42:15.30 CSV.dial between air and CTV @22:42:15.62 AIV.dial :bypass @22:42:15.70 Beginning samplePassC @22:42:15.71 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @22:42:15.79 Hand.open @22:42:16.23 Elbow.seek FlushPuck::Garage @22:42:18.20 Elbow.reconfigure LooseElbow @22:42:18.31 Forearm.seek :garage @22:42:19.30 Hand.close @22:42:20.10 Forearm.seek :retract @22:42:21.09 Elbow.reconfigure TightElbow @22:42:21.14 Elbow.seek CC @22:42:23.99 Elbow.reconfigure LooseElbow @22:42:24.09 Forearm.reconfigure TightForearm @22:42:24.14 Forearm.seek CC @22:42:25.47 CC.seek :closed @22:42:29.73 Collection Clamp onSpring/offSpring for 6/32 samples, on/off torque 1.72 @22:42:29.81 Hand.open @22:42:30.54 Forearm.reconfigure LooseForearm @22:42:30.59 Forearm.seek :retract @22:42:32.17 AWV.select :air @22:42:32.18 AIV.dial :bypass @22:42:32.19 PSV.dial PSR @22:42:33.47 Pulling 1.5ml of PSR.flush @22:42:33.48 PSR.select :flush @22:42:33.62 PS.seek 1.5ml @22:42:49.66 delay 5 seconds @22:42:54.69 PSV.dial :waste1 @22:42:55.16 PS.seek 0ml @22:43:11.19 PSV.dial PSR @22:43:11.63 PSR.select :air @22:43:11.76 PS.seek 2.5ml @22:43:37.92 PRV.dial CBV @22:43:37.98
CBV.dial PRV @22:43:38.72 PSV.dial :mixloop @22:43:39.68 CTV.dial :air,avoiding: :intake @22:43:39.69 PTV.dial :PRVmixing @22:43:39.80 PS.seek 0ml @22:44:05.88 PSV.dial :air @22:44:06.31 PS.seek 2.5ml @22:44:32.41 PSV.dial :mixloop @22:44:32.86 PS.seek 0ml @22:44:59.16 PSV.dial :air @22:44:59.61 PS.seek 2.5ml @22:45:25.76 PSV.dial :mixloop @22:45:26.22 PS.seek 0ml @22:45:52.35 Garaging FlushPuck in Collection Clamp @22:45:52.43 Elbow.reconfigure TightElbow @22:45:52.48 Elbow.seek CC @22:45:52.72 Elbow.reconfigure LooseElbow @22:45:52.82 Forearm.seek CC @22:45:53.88 Hand.close @22:45:54.47 CC.seek :open @22:45:58.80 Forearm.seek :retract @22:45:59.83 Elbow.reconfigure TightElbow @22:45:59.88 Elbow.seek :garage @22:46:02.92 Elbow.reconfigure LooseElbow @22:46:03.03 Forearm.reconfigure TightForearm @22:46:03.08 Forearm.seek :garage @22:46:04.23 Hand.open @22:46:04.92 Forearm.reconfigure LooseForearm @22:46:04.97 Forearm.seek :retract @22:46:06.04 Connecting PS to CS to transfer sample @22:46:06.05 CBV.dial AIV @22:46:06.06 AIV.dial :bypass @22:46:06.08 PSV.dial :mixloop @22:46:06.09 PTV.dial :PRVmixing @22:46:06.10 PRV.dial AIV @22:46:06.11 CTV.dial :air,avoiding: :intake @22:46:06.13 Loading lyfil puck from tube 2 into Collection Clamp @22:46:06.39 SC.reconfigure SCtight @22:46:06.44 SC.seek 2 @22:46:08.59 SC.reconfigure SCloose @22:46:08.82 Elbow.reconfigure TightElbow @22:46:08.86 Elbow.seek 2 @22:46:10.90 Forearm.reconfigure TightForearm @22:46:10.95 Forearm.seek 2 + 700 counts @22:46:12.09 SE.reconfigure SEpick @22:46:12.17 SE.seek beamBreak @22:46:13.83 SE.configure SEconfig @22:46:13.99 Elbow.seek 2 @22:46:14.80 Forearm.seek 2 + 300 counts @22:46:14.97 Forearm.reconfigure LooseForearm @22:46:15.02 Hand.close @22:46:15.62 SE.seek :down @22:46:15.87
Forearm.seek :retract @22:46:16.82 Elbow.seek CC @22:46:17.16 SE.coast @22:46:18.50
Elbow.reconfigure LooseElbow @22:46:18.61 Forearm.reconfigure TightForearm @22:46:18.66 Forearm.seek CC @22:46:20.03 CC.seek :closed @22:46:24.30 Collection Clamp onSpring/offSpring for 6/32 samples, on/off torque 1.75 @22:46:24.31 Hand.open @22:46:25.04 Forearm.reconfigure LooseForearm @22:46:25.09 Forearm.seek :retract @22:46:26.16 SC.coast @22:46:26.20 CTV.dial CSV,avoiding: :intake @22:46:27.46 CSV.dial CTV @22:46:27.78 CS.seek 5ml @22:46:41.58 delay 1:20 @22:48:01.62 CS.seek 2.5ml @22:48:08.96 delay 40 seconds @22:48:27.39 BACfans.email "Started mfb in tube 2 at 20:52:30CEST10-Sep-19\n{1 => 6, 2 => 16}\nCan@20:48:26, 16.6C, 79% humidity, 14.6psia, 15.186V, 0.809A, 0.809A avg, 12.28W",Subject: "pcrBAC sampling at most 2000ml, wcr at most 2000ml" @22:48:27.42 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @22:48:27.43 Retry #3 of 10 after 10:00 @22:48:48.99
CS.seek 0ml @22:48:56.52 delay 1:00 @22:49:56.55 PSV.dial :air @22:49:57.00 PS.seek 2.5ml @22:50:23.09 PSV.dial :mixloop @22:50:23.54 delay 3 seconds @22:50:26.57 PSV.dial :air @22:50:27.01 delay 2 seconds @22:50:29.03 PSV.dial :mixloop @22:50:29.48 delay 3 seconds @22:50:32.51 PSV.dial :waste1 @22:50:33.71 PS.seek :empty @22:50:59.88 CBV.dial :waste1 @22:51:00.33 delay 5 seconds @22:51:05.36 CBV.dial AIV @22:51:05.82 delay 10 seconds @22:51:15.85 CBV.dial :waste1 @22:51:16.30 delay 10 seconds @22:51:26.33 CBV.dial AIV @22:51:26.80 delay 10 seconds @22:51:36.83 CTV.dial CSV,avoiding: :intake @22:51:36.84 CBV.dial between AIV and waste1 @22:51:37.18 CSV.dial :air @22:51:37.77 CS.seek 10ml @22:52:04.33 CSV.dial CTV @22:52:04.77 CS.seek 0ml @22:52:31.49 CBV.dial :waste1 @22:52:31.79 delay 10 seconds @22:52:41.82 CTV.dial :air,avoiding: :intake @22:52:43.14 Storing lyfil puck in Collection Clamp to tube 1 @22:52:43.33 SC.reconfigure SCtight @22:52:43.38 SC.seek 1 @22:52:45.51 SC.reconfigure SCloose @22:52:45.74 Elbow.reconfigure TightElbow @22:52:45.79 Elbow.seek 1 @22:52:48.22 Forearm.reconfigure TightForearm @22:52:48.28 Forearm.seek 1 + 700 counts @22:52:49.27 SE.reconfigure SEpick @22:52:49.35 SE.seek beamBreak @22:52:51.78 SE.configure SEconfig @22:52:51.95 Elbow.reconfigure LimpElbow @22:52:52.00 Forearm.reconfigure LooseForearm @22:52:52.04 Forearm.seek 1 @22:52:52.37 Elbow.hold @22:52:52.44 Elbow.reconfigure TightElbow @22:52:52.55 SE.seek 6.08 Pucks below Top Plate @22:52:53.03 Forearm.force -20 @22:52:53.07 Forearm.seek 1 + 340 counts @22:52:53.39 Forearm.reconfigure TightForearm @22:52:53.44 Forearm.seek 1 + 700 counts @22:52:53.77 SE.seek 7.08 Pucks below Top Plate @22:52:54.46 Forearm.reconfigure LooseForearm @22:52:54.51 Forearm.seek :retract @22:52:55.45 Elbow.seek CC @22:52:57.14 Elbow.reconfigure LooseElbow @22:52:57.25 Forearm.seek CC @22:52:58.34 Hand.close @22:52:58.98 CC.seek :open @22:53:03.44 Forearm.seek :retract @22:53:04.50 Elbow.reconfigure TightElbow @22:53:04.55 Elbow.seek 1 @22:53:06.46 Elbow.reconfigure LimpElbow @22:53:06.51 Forearm.seek 1 @22:53:07.56 Elbow.hold @22:53:07.63 Elbow.reconfigure TightElbow @22:53:07.68 Hand.open @22:53:08.49 Forearm.force -20 @22:53:08.54 Forearm.seek 1 + 342 counts @22:53:08.86 Forearm.reconfigure TightForearm @22:53:08.90 Forearm.seek 1 + 700 counts @22:53:09.30 SE.seek :down @22:53:10.45
Forearm.reconfigure LooseForearm @22:53:10.50 Forearm.seek :retract @22:53:11.44 SE.coast @22:53:11.50
SC.coast @22:53:11.53 PSV.dial :air @22:53:12.57 PS.seek 0.499ml @22:53:18.45 delay 3 seconds @22:53:21.47 PSV.dial :mixloop @22:53:21.90 PRV.dial :air @22:53:22.34 delay 3 seconds @22:53:25.37 PRV.dial CBV @22:53:25.81 CBV.dial AIV @22:53:26.27 PRV.dial between CBV and air @22:53:26.59 PRV.dial :air @22:53:26.99 PS.reconfigure PSslow @22:53:27.04 PS.seek 0.599ml @22:53:29.51 PRV.dial :waste2 @22:53:30.60 PS.reconfigure PSconfig @22:53:30.65 PS.seek :empty @22:53:37.51 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @22:53:37.59 Hand.open @22:53:38.00 Elbow.seek FlushPuck::Garage @22:53:39.84 Elbow.reconfigure LooseElbow @22:53:39.95 Forearm.seek :garage @22:53:40.98 Hand.close @22:53:41.75 Forearm.seek :retract @22:53:42.76 Elbow.reconfigure TightElbow @22:53:42.81 Elbow.seek CC @22:53:45.69 Elbow.reconfigure LooseElbow @22:53:45.79 Forearm.reconfigure TightForearm @22:53:45.84 Forearm.seek CC @22:53:47.20 CC.seek :closed @22:53:51.46 Collection Clamp onSpring/offSpring for 6/32 samples, on/off torque 1.71 @22:53:51.54 Hand.open @22:53:52.18 Forearm.reconfigure LooseForearm @22:53:52.23 Forearm.seek :retract @22:53:53.34 Clearing PML for modified lysate @22:53:53.35 CTV.dial :air,avoiding: :intake @22:53:53.36 PRV.dial :waste2 @22:53:53.38 PTV.dial :PRV,avoiding: :puck @22:53:54.07 delay 2 seconds @22:53:56.09 PTV.dial :PRVmixing,avoiding: :puck @22:53:56.79 delay 2 seconds @22:53:58.82 PSV.dial :PML @22:53:58.83 delay 2 seconds @22:54:00.86 PRV.dial :air @22:54:01.57 delay 2 seconds @22:54:03.66 PS.seek 1.499ml @22:54:19.67 delay 5 seconds @22:54:24.71 PS.seek 2.5ml @22:54:35.70 PSV.dial :air @22:54:36.16 PS.seek 0ml @22:55:02.26 mixing lysate with speDil @22:55:02.27 PSV.dial :PML @22:55:02.71 CBV.dial :PRV @22:55:02.72 PRV.dial :air @22:55:02.74 delay 2 seconds @22:55:04.76 PRV.dial :speDil @22:55:05.21 delay 2 seconds @22:55:07.30 PS.reconfigure PSslow1 @22:55:07.35 PS.seek 0.225ml @22:55:31.01 delay 5 seconds @22:55:36.04 PRV.dial CBV @22:55:36.76 delay 2 seconds @22:55:38.84 PS.seek 0.475ml @22:56:04.51 delay 5 seconds @22:56:09.54 PRV.dial :air @22:56:10.02 delay 2 seconds @22:56:12.06 PS.seek 0.965ml @22:57:02.04 delay 10 seconds @22:57:12.07 PRV.dial CBV @22:57:12.53 CTV.dial :air,avoiding: :intake @22:57:12.54 PSV.dial :air @22:57:13.00 PS.reconfigure PSconfig @22:57:13.05 PS.seek 2.5ml @22:57:29.48 delay 3 seconds @22:57:32.50 PSV.dial PTV @22:57:33.70 PTV.dial :PRV,avoiding: :puck @22:57:34.41 PS.seek 0ml @22:58:00.58 PSV.dial :air @22:58:01.29 PS.seek 2.5ml @22:58:27.45 delay 3 seconds @22:58:27.47 BACfans.email "Started mfb in tube 2 at 20:52:30CEST10-Sep-19\n{1 => 6, 2 => 16}\nCan@20:48:26, 16.6C, 79% humidity, 14.6psia, 15.186V, 0.809A, 0.809A avg, 12.28W",Subject: "pcrBAC sampling at most 2000ml, wcr at most 2000ml" @22:58:27.51 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @22:58:27.52 Retry #4 of 10 after 10:00 @22:58:30.47
PSV.dial PTV @22:58:31.17 PTV.dial :PRV,avoiding: :puck @22:58:31.19 PS.seek 0ml @22:58:57.51 Pushing modified lysate into AIV Injection Loop @22:58:57.52 CBV.dial AIV @22:58:57.53 AIV.dial :bypass @22:58:57.55 PRV.dial CBV @22:58:57.56 PSV.dial :air @22:58:58.27 PS.seek 2.5ml @22:59:24.54 delay 3 seconds @22:59:27.56 PSV.dial :PML @22:59:28.00 PTV.dial :PRVmixing,avoiding: :puck @22:59:28.77 PS.reconfigure PSslow1 @22:59:28.82 PS.seek 1.74ml @23:00:46.20 delay 5 seconds @23:00:51.23 AIV.dial :sample @23:00:51.78 AWV.select :waste @23:00:51.95 PS.reconfigure PSslow2 @23:00:52.00 PS.seek 1.22ml @23:02:40.58 delay 15 seconds @23:02:55.61 AIV.dial :bypass @23:02:56.15 PS.reconfigure PSconfig @23:02:56.21 PS.seek 0ml @23:03:09.39 PSV.dial :air @23:03:09.84 PS.seek 2.5ml @23:03:35.92 PSV.dial :PML @23:03:36.37 PS.seek 0ml @23:04:02.48 AIV.dial :coilToMFB @23:04:02.49 AWV.select :air @23:04:02.66 Extracting Nucleic Acid from AIV @23:04:02.69
Beginning samplePassD @23:04:02.76 SPE.hold 2:46:40,55C @23:04:02.81 ASV.dial :SPEdelivery @23:04:02.89
AWV.select :air @23:04:02.90 AIV.dial :bypass @23:04:02.92 PSV.dial PSR @23:04:03.20 delay 20 seconds @23:04:03.97
Pulling 1.5ml of PSR.killMFB @23:04:03.98 PSR.select :killMFB @23:04:04.11 PS.seek 1.5ml @23:04:20.14 delay 5 seconds @23:04:23.23 primeLysateINT @23:04:23.24 AWV.select :air @23:04:23.25 AIV.dial :coilToMFB @23:04:23.27 ATV.dial AIV @23:04:24.90 AS.reconfigure ASslow1 @23:04:24.95 AS.seek 0.299ml @23:04:25.16
PSV.dial :waste1 @23:04:25.62 PS.seek 0ml @23:04:41.70 PSV.dial PSR @23:04:42.16 PSR.select :air @23:04:42.29 PS.seek 2.5ml @23:05:08.45 PRV.dial CBV @23:05:08.48
CBV.dial PRV @23:05:08.49 PSV.dial :mixloop @23:05:09.49 CTV.dial :air,avoiding: :intake @23:05:09.50 PTV.dial :PRVmixing @23:05:09.52 PS.seek 0ml @23:05:25.61 delay 10 seconds @23:05:35.64
PSV.dial PSR @23:05:35.68 ATV.dial :air2 @23:05:36.48 AS.seek 0.329ml @23:05:36.69
Pulling 1.5ml of PSR.flush @23:05:36.70 PSR.select :flush @23:05:36.83 PS.seek 1.5ml @23:05:42.78 ATV.dial :waste2 @23:05:43.31 AS.reconfigure ASnorm @23:05:43.36 AS.seek :empty @23:05:52.95
delay 5 seconds @23:05:57.98 PSV.dial :waste1 @23:05:58.96 PS.seek 0ml @23:06:00.39 Lysate primed! @23:06:00.40 AFV.select :holdingCoil @23:06:00.41 ATV.dial :air2 @23:06:01.02 AS.reconfigure ASslow1 @23:06:01.07 AS.seek 0.069ml @23:06:14.95
PSV.dial PSR @23:06:15.43 PSR.select :air @23:06:15.50 delay 3 seconds @23:06:15.56
PS.seek 2.5ml @23:06:18.53 ATV.dial :AIV @23:06:19.34 AS.seek 0.469ml @23:06:41.64 PRV.dial CBV @23:06:41.67
CBV.dial PRV @23:06:41.68 PSV.dial :mixloop @23:06:42.66 CTV.dial :air,avoiding: :intake @23:06:42.67 PTV.dial :PRVmixing @23:06:42.70 PS.seek 0ml @23:07:08.89 PSV.dial PSR @23:07:09.93 Pulling 1.5ml of PSR.flush PSR.select :flush @23:07:10.06 PS.seek 1.5ml @23:07:26.02 delay 5 seconds @23:07:31.05 PSV.dial :waste1 @23:07:31.51 PS.seek 0ml @23:07:40.06 delay 5 seconds @23:07:45.15 Solid Phase Extraction Heater at 53.3C @23:07:45.16 ATV.dial ASV @23:07:45.55 ASV.dial :SPEdelivery @23:07:45.63 AS.seek 0.041ml @23:07:47.49
PSV.dial PSR @23:07:47.94 PSR.select :air @23:07:48.07 PS.seek 2.5ml @23:08:14.17 PRV.dial CBV @23:08:14.20
CBV.dial PRV @23:08:14.21 PSV.dial :mixloop @23:08:15.17 CTV.dial :air,avoiding: :intake @23:08:15.18 PTV.dial :PRVmixing @23:08:15.21 PS.seek 0ml @23:08:27.55 BACfans.email "Started mfb in tube 2 at 20:52:30CEST10-Sep-19\n{1 => 6, 2 => 16}\nCan@20:48:26, 16.6C, 79% humidity, 14.6psia, 15.186V, 0.809A, 0.809A avg, 12.28W",Subject: "pcrBAC sampling at most 2000ml, wcr at most 2000ml" @23:08:27.58 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @23:08:27.60 Retry #5 of 10 after 10:00 @23:08:41.30
PSV.dial PSR @23:08:42.34 Pulling 1.5ml of PSR.flush @23:08:42.35 PSR.select :flush @23:08:42.48 PS.seek 1.5ml @23:08:58.58 delay 5 seconds @23:09:03.61 PSV.dial :waste1 @23:09:04.06 PS.seek 0ml @23:09:11.99 delay 10 seconds @23:09:20.11
PSV.dial PSR @23:09:20.55 PSR.select :air @23:09:20.68 PS.seek 2.5ml @23:09:22.01 rinseLysateLineINT @23:09:22.02 AFV.select :carrierSolution @23:09:22.13 delay 2 seconds @23:09:24.15 AS.reconfigure ASconfig @23:09:24.20 AS.seek 1ml @23:09:46.86 PRV.dial CBV @23:09:46.89
CBV.dial PRV @23:09:46.90 PSV.dial :mixloop @23:09:47.86 CTV.dial :air,avoiding: :intake @23:09:47.87 PTV.dial :PRVmixing @23:09:47.89 PS.reconfigure PSslow @23:09:47.94 PS.seek 1.2ml @23:10:14.77 PSV.dial :waste1 @23:10:16.00 PS.reconfigure PSconfig @23:10:16.05 PS.seek 0ml @23:10:19.58 delay 1 second @23:10:20.60 AFV.select :holdingCoil @23:10:20.70 delay 1 second @23:10:21.73 ATV.dial AIV @23:10:22.25 AIV.dial :coilToMFB @23:10:22.26 AWV.select :waste @23:10:22.38 AS.reconfigure ASslow0 @23:10:22.42 AS.seek 0ml @23:10:29.08
PSV.dial :air @23:10:30.04 PS.seek 2.5ml @23:10:56.14 PSV.dial :mixloop @23:10:56.58 PS.reconfigure PSslow @23:10:56.62 PS.seek 0ml @23:11:47.89 PSV.dial :air @23:11:48.86 PS.reconfigure PSconfig @23:11:48.90 PS.seek 2.5ml @23:12:03.31 ATV.dial :air2 @23:12:03.98 AS.reconfigure ASslow1 @23:12:04.03 AS.seek 0.05ml @23:12:14.41 ATV.dial :AIV @23:12:15.11
PSV.dial :mixloop @23:12:15.26 AS.seek 0.01ml @23:12:15.58
PS.reconfigure PSslow @23:12:15.62 PS.seek 0ml @23:12:23.52 ATV.dial :waste @23:12:25.09 AS.seek :empty @23:12:27.49 ethanol wash @23:12:27.50 ATV.dial :air2 @23:12:28.71 AS.reconfigure ASslow2 @23:12:28.76 AS.seek 0.009ml @23:12:39.16 delay 3 seconds @23:12:42.19 Pulling 0.4ml of Ethanol Wash @23:12:42.20 ATV.dial :ethanol @23:12:42.99 AS.reconfigure ASslow1 @23:12:43.04 AS.seek 0.409ml @23:13:06.68
Cleaning Collection Syringe @23:13:06.69 CSV.dial CSR @23:13:07.19 Pulling 3.999ml of CSR.flush @23:13:07.20 CSR.select :flush @23:13:07.33 CS.seek 4ml @23:13:18.57 delay 5 seconds @23:13:23.60 CSR.select :air @23:13:23.73 CS.seek 10ml @23:13:40.01 CSV.dial CTV @23:13:40.04
CTV.dial CSV @23:13:41.38 CBV.dial :waste1 @23:13:41.86 CS.seek 0ml @23:14:03.72 delay 3 seconds @23:14:06.75 ATV.dial :air1 @23:14:07.13 delay 3 seconds @23:14:08.51
CSV.dial CSR @23:14:09.04 Pulling 4ml of CSR.flush CSR.select :flush @23:14:09.17 CS.seek 4ml @23:14:10.22 AS.seek 0.434ml @23:14:15.53 delay 3 seconds @23:14:18.56 ATV.dial :ethanol @23:14:19.15 AS.seek 0.419ml @23:14:20.41
delay 5 seconds @23:14:22.46 delay 3 seconds @23:14:25.44
CSR.select :air @23:14:25.49 ATV.dial ASV @23:14:25.56
CS.seek 10ml @23:14:26.76 AS.seek 0.092ml @23:14:41.82 CSV.dial CTV @23:14:41.85
CTV.dial CSV @23:14:42.30 CBV.dial :waste1 @23:14:42.32 CS.seek 0ml @23:15:08.98 Garaging FlushPuck in Collection Clamp @23:15:09.05 Elbow.reconfigure TightElbow @23:15:09.10 Elbow.seek CC @23:15:09.35 Elbow.reconfigure LooseElbow @23:15:09.46 Forearm.seek CC @23:15:10.53 Hand.close @23:15:11.14 CC.seek :open @23:15:15.49 Forearm.seek :retract @23:15:16.54 Elbow.reconfigure TightElbow @23:15:16.59 Elbow.seek :garage @23:15:19.56 Elbow.reconfigure LooseElbow @23:15:19.66 Forearm.reconfigure TightForearm @23:15:19.71 Forearm.seek :garage @23:15:20.90 Hand.open @23:15:21.58 Forearm.reconfigure LooseForearm @23:15:22.16 Forearm.seek :retract @23:15:23.26 Whole Cell Archive sampling 2000ml @23:15:23.28 Loading wcr puck from tube 2 into Collection Clamp @23:15:23.55 SC.reconfigure SCtight @23:15:23.60 SC.seek 2 @23:15:25.72 SC.reconfigure SCloose @23:15:25.97 Elbow.reconfigure TightElbow @23:15:26.02 Elbow.seek 2 @23:15:28.04 Forearm.reconfigure TightForearm @23:15:28.09 Forearm.seek 2 + 700 counts @23:15:29.08 SE.reconfigure SEpick @23:15:29.17 SE.seek beamBreak @23:15:30.94 SE.configure SEconfig @23:15:31.12 Elbow.seek 2 @23:15:31.34 Forearm.seek 2 + 300 counts @23:15:31.62 Forearm.reconfigure LooseForearm @23:15:31.67 Hand.close @23:15:32.28 SE.seek :down @23:15:32.52 Forearm.seek :retract @23:15:32.80 delay 3 seconds @23:15:33.51 Elbow.seek CC @23:15:33.90 SE.coast @23:15:35.16 Elbow.reconfigure LooseElbow @23:15:35.27 Forearm.reconfigure TightForearm @23:15:35.32 Forearm.seek CC @23:15:35.83 ATV.dial :waste2 @23:15:36.64 AS.reconfigure ASconfig @23:15:36.76 AS.seek :empty @23:15:36.88 CC.seek :closed @23:15:41.14 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.64 @23:15:41.15 Hand.open @23:15:41.83 Forearm.reconfigure LooseForearm @23:15:41.88 Forearm.seek :retract @23:15:42.41 ATV.dial :air2 @23:15:42.80 AS.reconfigure ASslow1 @23:15:42.85 AS.seek 1ml @23:15:42.93 SC.coast @23:15:42.97 collectSample 2000ml w/bubble point of 55psi @23:15:42.99 Priming 7ml @23:15:43.00 Intake.select :close @23:15:43.01 Exhaust.select :close @23:15:43.02 SS.hold @23:15:43.27 Calibrating pressure sensors @23:15:43.28 CTV.dial :air @23:15:44.52 SSV.dial :spit,avoiding: :exhaust @23:15:44.55 SS.reconfigure SSfast @23:15:44.59 SS.seek 5ml @23:15:47.36 SSV.dial CBV @23:15:47.40 CBV.dial SSV @23:15:49.35 delay 3.8 seconds @23:15:53.30 SSV.dial :spit @23:15:54.56 SS.seek 0ml @23:15:57.30 SSV.dial CBV @23:15:58.58 CTV.dial :intake @23:15:59.33 SSV.dial CBV @23:15:59.35 CBV.dial SSV @23:15:59.38 Allowing 40s for 7.0ml of remaining 7.0ml @23:15:59.39 Intake.select :open @23:16:01.57 Intake @32.4psia, vacuum from 20.0 to 24.0psi @23:16:01.59 SS.reconfigure SSinhale.with maxSpeed: 150 @23:16:01.64 SS.seek 7ml @23:16:05.34 Vacuum too high -- waiting up to 40s @23:16:08.25 Intake @32.3psia, vacuum from 20.0 to 24.0psi @23:16:08.27 SS.reconfigure SSinhale.with maxSpeed: 135 @23:16:08.31 SS.seek 7ml @23:16:11.27 Stroke completed -- waiting for pressure to equalize ... @23:16:16.43 Intake.select :close @23:16:16.60 SSV.dial :exhaust @23:16:17.36 Exhaust.select :open @23:16:17.48 SS.reconfigure SSexhale @23:16:17.53 SS.seek 0ml @23:16:21.04 Exhaust.select :close @23:16:21.15 Sampling 2000.0ml @23:16:21.17 Intake.select :close @23:16:21.18 Exhaust.select :close @23:16:21.19 SS.reconfigure SSconfig @23:16:21.24 SS.hold @23:16:21.48 CTV.dial :intake @23:16:21.51 SSV.dial CBV @23:16:21.53 CBV.dial SSV @23:16:22.29 Allowing 130s for 25.0ml of remaining 2000.0ml @23:16:22.30 Intake.select :open @23:16:24.49 Intake @32.3psia, vacuum from 20.0 to 24.0psi @23:16:24.51 SS.reconfigure SSinhale.with maxSpeed: 135 @23:16:24.56 SS.seek 25ml @23:16:28.42 Vacuum too high -- waiting up to 40s @23:16:30.32 Intake @32.1psia, vacuum from 20.0 to 24.0psi @23:16:30.34 SS.reconfigure SSinhale.with maxSpeed: 122 @23:16:30.38 SS.seek 25ml @23:16:33.33 Vacuum too high -- waiting up to 40s @23:16:35.27 SS.reconfigure SSinhale.with maxSpeed: 110 @23:16:35.33 SS.seek 25ml @23:16:38.62 Vacuum too high -- waiting up to 40s @23:16:40.50 Intake @32.0psia, vacuum from 20.0 to 24.0psi @23:16:40.52 SS.reconfigure SSinhale.with maxSpeed: 99 @23:16:40.57 SS.seek 25ml @23:16:43.96 Vacuum too high -- waiting up to 40s @23:16:45.61 Intake @32.0psia, vacuum from 20.0 to 24.0psi @23:16:45.63 SS.reconfigure SSinhale.with maxSpeed: 89 @23:16:45.69 SS.seek 25ml @23:16:49.16 Vacuum too high -- waiting up to 40s @23:16:50.80 Intake @32.2psia, vacuum from 20.0 to 24.0psi @23:16:50.82 SS.reconfigure SSinhale.with maxSpeed: 80 @23:16:50.86 SS.seek 25ml @23:16:54.50 Vacuum too high -- waiting up to 40s @23:16:56.13 Intake @32.2psia, vacuum from 20.0 to 24.0psi @23:16:56.15 SS.reconfigure SSinhale.with maxSpeed: 72 @23:16:56.20 SS.seek 25ml @23:17:00.52 Stroke completed -- waiting for pressure to equalize ... @23:17:04.39 Intake.select :close @23:17:04.55 SSV.dial :exhaust @23:17:05.32 Exhaust.select :open @23:17:05.44 SS.reconfigure SSexhale @23:17:05.49 SS.seek 0ml @23:17:14.30 Exhaust.select :close @23:17:14.42 SSV.dial CBV @23:17:14.44 CBV.dial SSV @23:17:15.19 Allowing 130s for 25.0ml of remaining 1975.0ml @23:17:15.20 Intake.select :open @23:17:17.39 Intake @32.3psia, vacuum from 20.0 to 24.0psi @23:17:17.41 SS.reconfigure SSinhale.with maxSpeed: 72 @23:17:17.46 SS.seek 25ml @23:17:23.43 Vacuum too high -- waiting up to 40s @23:17:24.83 Intake @31.9psia, vacuum from 20.0 to 24.0psi @23:17:24.85 SS.reconfigure SSinhale.with maxSpeed: 65 @23:17:24.90 SS.seek 25ml @23:17:32.12 Vacuum too high -- waiting up to 40s @23:17:33.49 Intake @32.0psia, vacuum from 20.0 to 24.0psi @23:17:33.51 SS.reconfigure SSinhale.with maxSpeed: 59 @23:17:33.56 SS.seek 25ml @23:17:44.34 Vacuum too high -- waiting up to 40s @23:17:45.72 Intake @31.9psia, vacuum from 20.0 to 24.0psi @23:17:45.74 SS.reconfigure SSinhale.with maxSpeed: 53 @23:17:45.79 SS.seek 25ml @23:17:52.57 Stroke completed -- waiting for pressure to equalize ... @23:17:55.82 Intake.select :close @23:17:55.98 SSV.dial :exhaust @23:17:56.72 Exhaust.select :open @23:17:56.84 SS.reconfigure SSexhale @23:17:56.89 SS.seek 0ml @23:18:05.71 Exhaust.select :close @23:18:05.82 SSV.dial CBV @23:18:05.85 CBV.dial SSV @23:18:06.63 Allowing 130s for 25.0ml of remaining 1950.0ml @23:18:06.65 Intake.select :open @23:18:08.82 Intake @32.3psia, vacuum from 20.0 to 24.0psi @23:18:08.84 SS.reconfigure SSinhale.with maxSpeed: 53 @23:18:08.89 SS.seek 25ml @23:18:27.62 BACfans.email "Started mfb in tube 2 at 20:52:30CEST10-Sep-19\n{1 => 6, 2 => 16}\nCan@20:48:26, 16.6C, 79% humidity, 14.6psia, 15.186V, 0.809A, 0.809A avg, 12.28W",Subject: "pcrBAC sampling at most 2000ml, wcr at most 2000ml" @23:18:27.65 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @23:18:27.67 Retry #6 of 10 after 10:00 @23:18:42.68 Stroke completed -- waiting for pressure to equalize ... @23:18:46.56 Intake.select :close @23:18:46.73 SSV.dial :exhaust @23:18:47.47 Exhaust.select :open @23:18:47.59 SS.reconfigure SSexhale @23:18:47.64 SS.seek 0ml @23:18:56.47 Exhaust.select :close @23:18:56.59 SSV.dial CBV @23:18:56.61 CBV.dial SSV @23:18:57.38 Allowing 130s for 25.0ml of remaining 1925.0ml @23:18:57.39 Intake.select :open @23:18:59.58 Intake @32.2psia, vacuum from 20.0 to 24.0psi @23:18:59.60 SS.reconfigure SSinhale.with maxSpeed: 53 @23:18:59.64 SS.seek 25ml @23:19:04.38 delay 5 seconds @23:19:09.41 ATV.dial ASV @23:19:10.35 ASV.dial :SPEdelivery @23:19:10.37 AS.seek 0.02ml @23:19:33.43 Stroke completed -- waiting for pressure to equalize ... @23:19:37.87 Intake.select :close @23:19:38.03 SSV.dial :exhaust @23:19:38.79 Exhaust.select :open @23:19:38.91 SS.reconfigure SSexhale @23:19:38.96 SS.seek 0ml @23:19:47.77 Exhaust.select :close @23:19:47.89 SSV.dial CBV @23:19:47.91 CBV.dial SSV @23:19:48.69 Allowing 130s for 25.0ml of remaining 1900.0ml @23:19:48.70 Intake.select :open @23:19:50.89 Intake @32.1psia, vacuum from 20.0 to 24.0psi @23:19:50.91 SS.reconfigure SSinhale.with maxSpeed: 53 @23:19:50.96 SS.seek 25ml @23:20:24.74 Stroke completed -- waiting for pressure to equalize ... @23:20:28.62 Intake.select :close @23:20:28.79 SSV.dial :exhaust @23:20:29.54 Exhaust.select :open @23:20:29.66 SS.reconfigure SSexhale @23:20:29.71 SS.seek 0ml @23:20:38.44 Exhaust.select :close @23:20:38.55 SSV.dial CBV @23:20:38.58 CBV.dial SSV @23:20:39.36 Allowing 130s for 25.0ml of remaining 1875.0ml @23:20:39.38 Intake.select :open @23:20:41.56 Intake @32.1psia, vacuum from 20.0 to 24.0psi @23:20:41.58 SS.reconfigure SSinhale.with maxSpeed: 53 @23:20:41.63 SS.seek 25ml @23:21:15.68 Stroke completed -- waiting for pressure to equalize ... @23:21:19.56 Intake.select :close @23:21:19.73 SSV.dial :exhaust @23:21:20.49 Exhaust.select :open @23:21:20.61 SS.reconfigure SSexhale @23:21:20.66 SS.seek 0ml @23:21:29.47 Exhaust.select :close @23:21:29.59 SSV.dial CBV @23:21:29.61 CBV.dial SSV @23:21:30.36 Allowing 130s for 25.0ml of remaining 1850.0ml @23:21:30.38 Intake.select :open @23:21:32.56 Intake @32.1psia, vacuum from 20.0 to 24.0psi @23:21:32.58 SS.reconfigure SSinhale.with maxSpeed: 53 @23:21:32.62 SS.seek 25ml @23:21:58.42 Vacuum too high -- waiting up to 40s @23:21:59.55 Intake @31.7psia, vacuum from 20.0 to 24.0psi @23:21:59.57 SS.reconfigure SSinhale.with maxSpeed: 48 @23:21:59.61 SS.seek 25ml @23:22:08.66 Stroke completed -- waiting for pressure to equalize ... @23:22:11.91 Intake.select :close @23:22:12.07 SSV.dial :exhaust @23:22:12.83 Exhaust.select :open @23:22:12.95 SS.reconfigure SSexhale @23:22:13.00 SS.seek 0ml @23:22:21.83 Exhaust.select :close @23:22:22.48 SSV.dial CBV @23:22:22.51 CBV.dial SSV @23:22:23.27 Allowing 130s for 25.0ml of remaining 1825.0ml @23:22:23.29 Intake.select :open @23:22:25.47 Intake @32.1psia, vacuum from 20.0 to 24.0psi @23:22:25.49 SS.reconfigure SSinhale.with maxSpeed: 48 @23:22:25.54 SS.seek 25ml @23:22:27.72 delay 5 seconds @23:22:32.75 ATV.dial :air2 @23:22:33.86 AS.seek 0.52ml @23:23:02.60 Stroke completed -- waiting for pressure to equalize ... @23:23:05.87 Intake.select :close @23:23:06.03 SSV.dial :exhaust @23:23:06.77 Exhaust.select :open @23:23:06.89 SS.reconfigure SSexhale @23:23:06.94 SS.seek 0ml @23:23:15.83 Exhaust.select :close @23:23:15.95 SSV.dial CBV @23:23:15.97 CBV.dial SSV @23:23:16.74 Allowing 130s for 25.0ml of remaining 1800.0ml @23:23:16.75 Intake.select :open @23:23:18.94 Intake @32.2psia, vacuum from 20.0 to 24.0psi @23:23:18.96 SS.reconfigure SSinhale.with maxSpeed: 48 @23:23:19.01 SS.seek 25ml @23:23:56.18 Stroke completed -- waiting for pressure to equalize ... @23:23:59.43 Intake.select :close @23:23:59.60 SSV.dial :exhaust @23:24:00.35 Exhaust.select :open @23:24:00.47 SS.reconfigure SSexhale @23:24:00.52 SS.seek 0ml @23:24:09.52 Exhaust.select :close @23:24:09.64 SSV.dial CBV @23:24:09.66 CBV.dial SSV @23:24:10.44 Allowing 130s for 25.0ml of remaining 1775.0ml @23:24:10.45 Intake.select :open @23:24:12.64 Intake @32.3psia, vacuum from 20.0 to 24.0psi @23:24:12.66 SS.reconfigure SSinhale.with maxSpeed: 48 @23:24:12.71 SS.seek 25ml @23:24:14.78 delay 5 seconds @23:24:19.81 ATV.dial ASV @23:24:20.75 ASV.dial :wasteR @23:24:21.11 AS.seek 0.3ml @23:24:49.63 Stroke completed -- waiting for pressure to equalize ... @23:24:52.87 Intake.select :close @23:24:53.03 SSV.dial :exhaust @23:24:53.78 Exhaust.select :open @23:24:53.90 SS.reconfigure SSexhale @23:24:53.95 SS.seek 0ml @23:25:02.78 Exhaust.select :close @23:25:02.90 SSV.dial CBV @23:25:02.92 CBV.dial SSV @23:25:03.69 Allowing 130s for 25.0ml of remaining 1750.0ml @23:25:03.70 Intake.select :open @23:25:05.60 delay 20 seconds @23:25:05.89 Intake @32.1psia, vacuum from 20.0 to 24.0psi @23:25:05.91 SS.reconfigure SSinhale.with maxSpeed: 48 @23:25:05.95 SS.seek 25ml @23:25:25.64 AS.seek 0.07ml @23:25:43.10 Stroke completed -- waiting for pressure to equalize ... @23:25:46.87 Intake.select :close @23:25:47.03 SSV.dial :exhaust @23:25:47.80 Exhaust.select :open @23:25:47.92 SS.reconfigure SSexhale @23:25:47.97 SS.seek 0ml @23:25:56.66 Exhaust.select :close @23:25:56.78 SSV.dial CBV @23:25:56.80 CBV.dial SSV @23:25:57.57 Allowing 130s for 25.0ml of remaining 1725.0ml @23:25:57.59 Intake.select :open @23:25:59.77 Intake @32.2psia, vacuum from 20.0 to 24.0psi @23:25:59.79 SS.reconfigure SSinhale.with maxSpeed: 48 @23:25:59.84 SS.seek 25ml @23:26:12.14 delay 20 seconds @23:26:32.17 ATV.dial :waste2 @23:26:32.89 AS.reconfigure ASconfig @23:26:32.95 AS.seek :empty @23:26:37.11 Stroke completed -- waiting for pressure to equalize ... @23:26:37.34 Elution @23:26:37.35 ATV.dial :air1 @23:26:38.48 AS.reconfigure ASslow1 @23:26:38.53 AS.seek 0.049ml @23:26:41.00 Intake.select :close @23:26:41.16 SSV.dial :exhaust @23:26:41.93 Exhaust.select :open @23:26:42.05 SS.reconfigure SSexhale @23:26:42.10 SS.seek 0ml @23:26:48.88 delay 3 seconds @23:26:50.94 Exhaust.select :close @23:26:51.06 SSV.dial CBV @23:26:51.08 CBV.dial SSV @23:26:51.85 Allowing 130s for 25.0ml of remaining 1700.0ml @23:26:51.86 Intake.select :open @23:26:51.92 ATV.dial :ElutionWater @23:26:52.57 AS.seek 0.109ml @23:26:54.05 Intake @32.4psia, vacuum from 20.0 to 24.0psi @23:26:54.07 SS.reconfigure SSinhale.with maxSpeed: 48 @23:26:54.12 SS.seek 25ml @23:27:04.91 delay 3 seconds @23:27:07.94 ATV.dial ASV,via: :air2 @23:27:09.88 ASV.dial :SPEelution @23:27:10.44 AFV.select :carrierSolution @23:27:10.60 AS.reconfigure ASnorm @23:27:10.66 AS.seek 0.759ml @23:27:31.30 Stroke completed -- waiting for pressure to equalize ... @23:27:35.17 Intake.select :close @23:27:35.33 SSV.dial :exhaust @23:27:36.10 Exhaust.select :open @23:27:36.22 SS.reconfigure SSexhale @23:27:36.27 SS.seek 0ml @23:27:43.71 delay 3 seconds @23:27:45.11 Exhaust.select :close @23:27:45.23 SSV.dial CBV @23:27:45.25 CBV.dial SSV @23:27:46.02 Allowing 130s for 25.0ml of remaining 1675.0ml @23:27:46.03 Intake.select :open @23:27:46.73 AFV.select :holdingCoil @23:27:46.90 AS.reconfigure ASslow1 @23:27:46.96 AS.seek 0.474ml @23:27:48.22 Intake @32.4psia, vacuum from 20.0 to 24.0psi @23:27:48.24 SS.reconfigure SSinhale.with maxSpeed: 48 @23:27:48.29 SS.seek 25ml @23:28:25.39 Stroke completed -- waiting for pressure to equalize ... @23:28:27.69 BACfans.email "Started mfb in tube 2 at 20:52:30CEST10-Sep-19\n{1 => 6, 2 => 16}\nCan@20:48:26, 16.6C, 79% humidity, 14.6psia, 15.186V, 0.809A, 0.809A avg, 12.28W",Subject: "pcrBAC sampling at most 2000ml, wcr at most 2000ml" @23:28:27.72 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @23:28:27.74 Retry #7 of 10 after 10:00 @23:28:29.22 Intake.select :close @23:28:29.38 SSV.dial :exhaust @23:28:30.13 Exhaust.select :open @23:28:30.25 SS.reconfigure SSexhale @23:28:30.30 SS.seek 0ml @23:28:39.11 Exhaust.select :close @23:28:39.22 SSV.dial CBV @23:28:39.25 CBV.dial SSV @23:28:40.02 Allowing 130s for 25.0ml of remaining 1650.0ml @23:28:40.04 Intake.select :open @23:28:42.22 Intake @32.3psia, vacuum from 20.0 to 24.0psi @23:28:42.24 SS.reconfigure SSinhale.with maxSpeed: 48 @23:28:42.29 SS.seek 25ml @23:28:44.56 delay 5 seconds @23:28:49.65 AS.reconfigure ASslow2 @23:28:49.70 AS.seek 0.439ml @23:29:19.39 Stroke completed -- waiting for pressure to equalize ... @23:29:23.35 Intake.select :close @23:29:23.51 SSV.dial :exhaust @23:29:24.25 Exhaust.select :open @23:29:24.37 SS.reconfigure SSexhale @23:29:24.43 SS.seek 0ml @23:29:25.02 delay 1:00 @23:29:33.37 Exhaust.select :close @23:29:33.51 SSV.dial CBV @23:29:33.53 CBV.dial SSV @23:29:34.30 Allowing 130s for 25.0ml of remaining 1625.0ml @23:29:34.31 Intake.select :open @23:29:36.49 Intake @32.3psia, vacuum from 20.0 to 24.0psi @23:29:36.51 SS.reconfigure SSinhale.with maxSpeed: 48 @23:29:36.56 SS.seek 25ml @23:30:13.67 Stroke completed -- waiting for pressure to equalize ... @23:30:18.17 Intake.select :close @23:30:18.33 SSV.dial :exhaust @23:30:19.11 Exhaust.select :open @23:30:19.23 SS.reconfigure SSexhale @23:30:19.28 SS.seek 0ml @23:30:25.05 pushing eluate to holding coil @23:30:25.12 AS.seek 0.279ml @23:30:27.86 Exhaust.select :close @23:30:27.98 SSV.dial CBV @23:30:28.00 CBV.dial SSV @23:30:28.77 Allowing 130s for 25.0ml of remaining 1600.0ml @23:30:28.78 Intake.select :open @23:30:30.97 Intake @32.4psia, vacuum from 20.0 to 24.0psi @23:30:30.99 SS.reconfigure SSinhale.with maxSpeed: 48 @23:30:31.03 SS.seek 25ml @23:31:08.47 Stroke completed -- waiting for pressure to equalize ... @23:31:13.56 Intake.select :close @23:31:13.73 SSV.dial :exhaust @23:31:14.49 Exhaust.select :open @23:31:14.61 SS.reconfigure SSexhale @23:31:14.66 SS.seek 0ml @23:31:23.46 Exhaust.select :close @23:31:23.58 SSV.dial CBV @23:31:23.60 CBV.dial SSV @23:31:24.36 Allowing 130s for 25.0ml of remaining 1575.0ml @23:31:24.38 Intake.select :open @23:31:26.55 Intake @32.4psia, vacuum from 20.0 to 24.0psi @23:31:26.58 SS.reconfigure SSinhale.with maxSpeed: 48 @23:31:26.63 SS.seek 25ml @23:31:59.77 Vacuum too high -- waiting up to 40s @23:32:01.40 Intake @32.1psia, vacuum from 20.0 to 24.0psi @23:32:01.42 SS.reconfigure SSinhale.with maxSpeed: 43 @23:32:01.47 SS.seek 25ml @23:32:06.28 Stroke completed -- waiting for pressure to equalize ... @23:32:09.52 Intake.select :close @23:32:09.69 SSV.dial :exhaust @23:32:10.46 Exhaust.select :open @23:32:10.58 SS.reconfigure SSexhale @23:32:10.63 SS.seek 0ml @23:32:19.74 Exhaust.select :close @23:32:19.86 SSV.dial CBV @23:32:19.88 CBV.dial SSV @23:32:20.64 Allowing 130s for 25.0ml of remaining 1550.0ml @23:32:20.65 Intake.select :open @23:32:22.84 Intake @32.5psia, vacuum from 20.0 to 24.0psi @23:32:22.86 SS.reconfigure SSinhale.with maxSpeed: 43 @23:32:22.91 SS.seek 25ml @23:33:03.75 Stroke completed -- waiting for pressure to equalize ... @23:33:06.26 delay 30 seconds @23:33:07.06 Intake.select :close @23:33:07.22 SSV.dial :exhaust @23:33:07.99 Exhaust.select :open @23:33:08.11 SS.reconfigure SSexhale @23:33:08.16 SS.seek 0ml @23:33:17.00 Exhaust.select :close @23:33:17.12 SSV.dial CBV @23:33:17.14 CBV.dial SSV @23:33:17.91 Allowing 130s for 25.0ml of remaining 1525.0ml @23:33:17.93 Intake.select :open @23:33:20.11 Intake @32.6psia, vacuum from 20.0 to 24.0psi @23:33:20.13 SS.reconfigure SSinhale.with maxSpeed: 43 @23:33:20.17 SS.seek 25ml @23:33:36.28 ATV.dial :waste,via: :air3 @23:33:37.86 AS.reconfigure ASconfig @23:33:37.91 AS.seek :empty @23:33:54.30 Parking Bleach in LysateSource line and SPEcolumn @23:33:54.31 ATV.dial :air3 @23:33:55.11 AS.reconfigure ASslow1 @23:33:55.16 AS.seek 0.029ml @23:34:01.50 Stroke completed -- waiting for pressure to equalize ... @23:34:01.54 ATV.dial :ElutionWater @23:34:02.19 AS.seek 0.059ml @23:34:05.49 Intake.select :close @23:34:05.65 SSV.dial :exhaust @23:34:06.41 Exhaust.select :open @23:34:06.53 SS.reconfigure SSexhale @23:34:06.59 SS.seek 0ml @23:34:08.52 ATV.dial :air3 @23:34:09.12 AS.seek 0.069ml @23:34:11.40 delay 2 seconds @23:34:13.43 ATV.dial :bleach @23:34:13.89 AS.seek 0.769ml @23:34:15.39 Exhaust.select :close @23:34:15.51 SSV.dial CBV @23:34:15.53 CBV.dial SSV @23:34:16.28 Allowing 130s for 25.0ml of remaining 1500.0ml @23:34:16.30 Intake.select :open @23:34:18.48 Intake @32.4psia, vacuum from 20.0 to 24.0psi @23:34:18.50 SS.reconfigure SSinhale.with maxSpeed: 43 @23:34:18.55 SS.seek 25ml @23:34:59.85 Stroke completed -- waiting for pressure to equalize ... @23:35:03.73 Intake.select :close @23:35:03.89 SSV.dial :exhaust @23:35:04.66 Exhaust.select :open @23:35:04.78 SS.reconfigure SSexhale @23:35:04.83 SS.seek 0ml @23:35:13.80 Exhaust.select :close @23:35:13.92 SSV.dial CBV @23:35:13.94 CBV.dial SSV @23:35:14.70 Allowing 130s for 25.0ml of remaining 1475.0ml @23:35:14.71 Intake.select :open @23:35:16.90 Intake @32.6psia, vacuum from 20.0 to 24.0psi @23:35:16.92 SS.reconfigure SSinhale.with maxSpeed: 43 @23:35:16.96 SS.seek 25ml @23:35:57.95 Stroke completed -- waiting for pressure to equalize ... @23:36:01.82 Intake.select :close @23:36:01.98 SSV.dial :exhaust @23:36:02.74 Exhaust.select :open @23:36:02.86 SS.reconfigure SSexhale @23:36:02.91 SS.seek 0ml @23:36:11.92 Exhaust.select :close @23:36:12.04 SSV.dial CBV @23:36:12.06 CBV.dial SSV @23:36:12.83 Allowing 130s for 25.0ml of remaining 1450.0ml @23:36:12.84 Intake.select :open @23:36:15.03 Intake @32.5psia, vacuum from 20.0 to 24.0psi @23:36:15.05 SS.reconfigure SSinhale.with maxSpeed: 43 @23:36:15.10 SS.seek 25ml @23:36:34.94 delay 3 seconds @23:36:37.97 ATV.dial :air3 @23:36:38.49 delay 3 seconds @23:36:41.58 AS.seek 0.794ml @23:36:46.91 delay 3 seconds @23:36:49.94 ATV.dial :bleach @23:36:50.53 AS.seek 0.779ml @23:36:53.82 delay 3 seconds @23:36:56.22 Stroke completed -- waiting for pressure to equalize ... @23:36:56.87 ATV.dial :AIV @23:36:58.17 AS.reconfigure ASconfig @23:36:58.22 AS.seek 0.015ml @23:37:00.29 Intake.select :close @23:37:00.45 SSV.dial :exhaust @23:37:01.22 Exhaust.select :open @23:37:01.35 SS.reconfigure SSexhale @23:37:01.40 SS.seek 0ml @23:37:10.04 Exhaust.select :close @23:37:10.16 SSV.dial CBV @23:37:10.18 CBV.dial SSV @23:37:10.94 Allowing 130s for 25.0ml of remaining 1425.0ml @23:37:10.95 Intake.select :open @23:37:13.14 Intake @32.7psia, vacuum from 20.0 to 24.0psi @23:37:13.16 SS.reconfigure SSinhale.with maxSpeed: 43 @23:37:13.21 SS.seek 25ml @23:37:42.97 ATV.dial :waste @23:37:44.38 AS.seek :empty @23:37:45.57 ATV.dial :air3 @23:37:46.23 AS.reconfigure ASslow1 @23:37:46.28 AS.seek 0.009ml @23:37:48.61 delay 2 seconds @23:37:50.64 ATV.dial :bleach @23:37:51.09 AS.seek 0.579ml @23:37:54.80 Stroke completed -- waiting for pressure to equalize ... @23:37:59.31 Intake.select :close @23:37:59.47 SSV.dial :exhaust @23:38:00.25 Exhaust.select :open @23:38:00.37 SS.reconfigure SSexhale @23:38:00.42 SS.seek 0ml @23:38:09.38 Exhaust.select :close @23:38:09.50 SSV.dial CBV @23:38:09.52 CBV.dial SSV @23:38:10.30 Allowing 130s for 25.0ml of remaining 1400.0ml @23:38:10.31 Intake.select :open @23:38:12.50 Intake @32.5psia, vacuum from 20.0 to 24.0psi @23:38:12.52 SS.reconfigure SSinhale.with maxSpeed: 43 @23:38:12.57 SS.seek 25ml @23:38:27.76 BACfans.email "Started mfb in tube 2 at 20:52:30CEST10-Sep-19\n{1 => 6, 2 => 16}\nCan@20:48:26, 16.6C, 79% humidity, 14.6psia, 15.186V, 0.809A, 0.809A avg, 12.28W",Subject: "pcrBAC sampling at most 2000ml, wcr at most 2000ml" @23:38:27.79 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @23:38:27.81 Retry #8 of 10 after 10:00 @23:38:53.86 Stroke completed -- waiting for pressure to equalize ... @23:38:58.38 Intake.select :close @23:38:58.54 SSV.dial :exhaust @23:38:59.31 Exhaust.select :open @23:38:59.43 SS.reconfigure SSexhale @23:38:59.48 SS.seek 0ml @23:39:08.16 Exhaust.select :close @23:39:08.28 SSV.dial CBV @23:39:08.30 CBV.dial SSV @23:39:09.06 Allowing 130s for 25.0ml of remaining 1375.0ml @23:39:09.07 Intake.select :open @23:39:11.26 Intake @32.6psia, vacuum from 20.0 to 24.0psi @23:39:11.28 SS.reconfigure SSinhale.with maxSpeed: 43 @23:39:11.33 SS.seek 25ml @23:39:45.97 delay 3 seconds @23:39:49.00 ATV.dial :air3 @23:39:49.52 delay 3 seconds @23:39:52.61 AS.seek 0.604ml @23:39:52.91 Stroke completed -- waiting for pressure to equalize ... @23:39:57.34 Intake.select :close @23:39:57.50 SSV.dial :exhaust @23:39:57.94 delay 3 seconds @23:39:58.27 Exhaust.select :open @23:39:58.39 SS.reconfigure SSexhale @23:39:58.44 SS.seek 0ml @23:40:00.97 ATV.dial :bleach @23:40:01.56 AS.seek 0.589ml @23:40:04.85 delay 3 seconds @23:40:07.55 Exhaust.select :close @23:40:07.67 SSV.dial CBV @23:40:07.69 CBV.dial SSV @23:40:07.87 ATV.dial :ASV @23:40:08.47 Allowing 130s for 25.0ml of remaining 1350.0ml @23:40:08.49 Intake.select :open @23:40:08.85 ASV.dial :wasteR @23:40:09.50 AS.seek 0ml @23:40:10.67 Intake @32.7psia, vacuum from 20.0 to 24.0psi @23:40:10.69 SS.reconfigure SSinhale.with maxSpeed: 43 @23:40:10.74 SS.seek 25ml @23:40:51.81 Stroke completed -- waiting for pressure to equalize ... @23:40:56.33 Intake.select :close @23:40:56.49 SSV.dial :exhaust @23:40:57.26 Exhaust.select :open @23:40:57.37 SS.reconfigure SSexhale @23:40:57.43 SS.seek 0ml @23:41:06.09 Exhaust.select :close @23:41:06.21 SSV.dial CBV @23:41:06.23 CBV.dial SSV @23:41:06.98 Allowing 130s for 25.0ml of remaining 1325.0ml @23:41:06.99 Intake.select :open @23:41:09.18 Intake @32.7psia, vacuum from 20.0 to 24.0psi @23:41:09.20 SS.reconfigure SSinhale.with maxSpeed: 43 @23:41:09.25 SS.seek 25ml @23:41:44.07 Vacuum too high -- waiting up to 40s @23:41:45.70 Intake @32.4psia, vacuum from 20.0 to 24.0psi @23:41:45.72 SS.reconfigure SSinhale.with maxSpeed: 39 @23:41:45.78 SS.seek 25ml @23:41:53.34 Stroke completed -- waiting for pressure to equalize ... @23:41:57.21 Intake.select :close @23:41:57.37 SSV.dial :exhaust @23:41:58.13 Exhaust.select :open @23:41:58.25 SS.reconfigure SSexhale @23:41:58.30 SS.seek 0ml @23:42:07.47 Exhaust.select :close @23:42:07.59 SSV.dial CBV @23:42:07.61 CBV.dial SSV @23:42:08.27 delay 1:00 @23:42:08.38 Allowing 130s for 25.0ml of remaining 1300.0ml @23:42:08.95 Intake.select :open @23:42:11.13 Intake @32.7psia, vacuum from 20.0 to 24.0psi @23:42:11.15 SS.reconfigure SSinhale.with maxSpeed: 39 @23:42:11.20 SS.seek 25ml @23:42:56.16 Stroke completed -- waiting for pressure to equalize ... @23:43:00.12 Intake.select :close @23:43:00.28 SSV.dial :exhaust @23:43:01.05 Exhaust.select :open @23:43:01.17 SS.reconfigure SSexhale @23:43:01.23 SS.seek 0ml @23:43:08.30 AFV.select :carrierSolution @23:43:08.40 delay 2 seconds @23:43:10.19 Exhaust.select :close @23:43:10.31 SSV.dial CBV @23:43:10.33 CBV.dial SSV @23:43:10.42 AS.reconfigure ASconfig @23:43:10.47 AS.seek 1ml @23:43:11.11 Allowing 130s for 25.0ml of remaining 1275.0ml @23:43:11.13 Intake.select :open @23:43:13.32 Intake @32.9psia, vacuum from 20.0 to 24.0psi @23:43:13.34 SS.reconfigure SSinhale.with maxSpeed: 39 @23:43:13.39 SS.seek 25ml @23:43:58.69 Stroke completed -- waiting for pressure to equalize ... @23:44:03.21 Intake.select :close @23:44:03.38 SSV.dial :exhaust @23:44:04.15 Exhaust.select :open @23:44:04.26 SS.reconfigure SSexhale @23:44:04.32 SS.seek 0ml @23:44:08.24 delay 1 second @23:44:09.26 AFV.select :holdingCoil @23:44:09.37 delay 1 second @23:44:10.39 ATV.dial ASV @23:44:10.40 ASV.dial :wasteR @23:44:10.42 AS.reconfigure ASslow1 @23:44:10.46 AS.seek 0ml @23:44:13.17 Exhaust.select :close @23:44:13.29 SSV.dial CBV @23:44:13.31 CBV.dial SSV @23:44:14.06 Allowing 130s for 25.0ml of remaining 1250.0ml @23:44:14.07 Intake.select :open @23:44:16.26 Intake @32.8psia, vacuum from 20.0 to 24.0psi @23:44:16.28 SS.reconfigure SSinhale.with maxSpeed: 39 @23:44:16.33 SS.seek 25ml @23:45:01.77 Stroke completed -- waiting for pressure to equalize ... @23:45:06.95 Intake.select :close @23:45:07.11 SSV.dial :exhaust @23:45:07.87 Exhaust.select :open @23:45:07.99 SS.reconfigure SSexhale @23:45:08.04 SS.seek 0ml @23:45:17.03 Exhaust.select :close @23:45:17.15 SSV.dial CBV @23:45:17.17 CBV.dial SSV @23:45:17.94 Allowing 130s for 25.0ml of remaining 1225.0ml @23:45:17.95 Intake.select :open @23:45:20.14 Intake @32.9psia, vacuum from 20.0 to 24.0psi @23:45:20.16 SS.reconfigure SSinhale.with maxSpeed: 39 @23:45:20.20 SS.seek 25ml @23:46:05.32 Stroke completed -- waiting for pressure to equalize ... @23:46:10.39 Intake.select :close @23:46:10.55 SSV.dial :exhaust @23:46:11.31 Exhaust.select :open @23:46:11.43 SS.reconfigure SSexhale @23:46:11.48 SS.seek 0ml @23:46:20.31 Exhaust.select :close @23:46:20.43 SSV.dial CBV @23:46:20.45 CBV.dial SSV @23:46:21.22 Allowing 130s for 25.0ml of remaining 1200.0ml @23:46:21.23 Intake.select :open @23:46:23.41 Intake @32.9psia, vacuum from 20.0 to 24.0psi @23:46:23.43 SS.reconfigure SSinhale.with maxSpeed: 39 @23:46:23.48 SS.seek 25ml @23:46:56.61 Vacuum too high -- waiting up to 40s @23:46:58.23 Intake @32.5psia, vacuum from 20.0 to 24.0psi @23:46:58.25 SS.reconfigure SSinhale.with maxSpeed: 35 @23:46:58.29 SS.seek 25ml @23:47:12.01 Stroke completed -- waiting for pressure to equalize ... @23:47:16.53 Intake.select :close @23:47:16.70 SSV.dial :exhaust @23:47:17.48 Exhaust.select :open @23:47:17.60 SS.reconfigure SSexhale @23:47:17.65 SS.seek 0ml @23:47:26.33 Exhaust.select :close @23:47:26.45 SSV.dial CBV @23:47:26.47 CBV.dial SSV @23:47:27.23 Allowing 130s for 25.0ml of remaining 1175.0ml @23:47:27.24 Intake.select :open @23:47:29.43 Intake @32.8psia, vacuum from 20.0 to 24.0psi @23:47:29.45 SS.reconfigure SSinhale.with maxSpeed: 35 @23:47:29.49 SS.seek 25ml @23:47:31.85 delay 20 seconds @23:47:51.88 ATV.dial :air3 @23:47:53.28 AS.seek 0.59ml @23:48:20.13 Stroke completed -- waiting for pressure to equalize ... @23:48:25.30 Intake.select :close @23:48:25.46 SSV.dial :exhaust @23:48:26.22 Exhaust.select :open @23:48:26.33 SS.reconfigure SSexhale @23:48:26.39 SS.seek 0ml @23:48:27.83 BACfans.email "Started mfb in tube 2 at 20:52:30CEST10-Sep-19\n{1 => 6, 2 => 16}\nCan@20:48:26, 16.6C, 79% humidity, 14.6psia, 15.186V, 0.809A, 0.809A avg, 12.28W",Subject: "pcrBAC sampling at most 2000ml, wcr at most 2000ml" @23:48:27.86 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @23:48:27.87 Retry #9 of 10 after 10:00 @23:48:35.22 Exhaust.select :close @23:48:35.83 SSV.dial CBV @23:48:35.87 CBV.dial SSV @23:48:36.64 Allowing 130s for 25.0ml of remaining 1150.0ml @23:48:36.66 Intake.select :open @23:48:38.84 Intake @33.0psia, vacuum from 20.0 to 24.0psi @23:48:38.86 SS.reconfigure SSinhale.with maxSpeed: 35 @23:48:38.91 SS.seek 25ml @23:49:29.55 Stroke completed -- waiting for pressure to equalize ... @23:49:34.72 Intake.select :close @23:49:34.88 SSV.dial :exhaust @23:49:35.64 Exhaust.select :open @23:49:35.76 SS.reconfigure SSexhale @23:49:35.81 SS.seek 0ml @23:49:44.86 Exhaust.select :close @23:49:44.98 SSV.dial CBV @23:49:45.00 CBV.dial SSV @23:49:45.76 Allowing 130s for 25.0ml of remaining 1125.0ml @23:49:45.78 Intake.select :open @23:49:47.96 Intake @32.9psia, vacuum from 20.0 to 24.0psi @23:49:47.98 SS.reconfigure SSinhale.with maxSpeed: 35 @23:49:48.02 SS.seek 25ml @23:49:52.26 delay 3 seconds @23:49:55.29 ATV.dial :ASV @23:49:56.60 ASV.dial :wasteR @23:49:56.69 AS.seek 0.09ml @23:50:38.44 Stroke completed -- waiting for pressure to equalize ... @23:50:44.23 Intake.select :close @23:50:44.40 SSV.dial :exhaust @23:50:45.15 Exhaust.select :open @23:50:45.27 SS.reconfigure SSexhale @23:50:45.32 SS.seek 0ml @23:50:54.14 Exhaust.select :close @23:50:54.26 SSV.dial CBV @23:50:54.28 CBV.dial SSV @23:50:55.05 Allowing 130s for 25.0ml of remaining 1100.0ml @23:50:55.06 Intake.select :open @23:50:57.25 Intake @33.0psia, vacuum from 20.0 to 24.0psi @23:50:57.27 SS.reconfigure SSinhale.with maxSpeed: 35 @23:50:57.31 SS.seek 25ml @23:51:37.49 delay 30 seconds @23:51:40.43 Vacuum too high -- waiting up to 40s @23:51:42.06 Intake @32.6psia, vacuum from 20.0 to 24.0psi @23:51:42.08 SS.reconfigure SSinhale.with maxSpeed: 32 @23:51:42.12 SS.seek 25ml @23:51:50.38 Stroke completed -- waiting for pressure to equalize ... @23:51:54.99 Intake.select :close @23:51:55.15 SSV.dial :exhaust @23:51:55.92 Exhaust.select :open @23:51:56.04 SS.reconfigure SSexhale @23:51:56.09 SS.seek 0ml @23:52:05.00 Exhaust.select :close @23:52:05.12 SSV.dial CBV @23:52:05.14 CBV.dial SSV @23:52:05.92 Allowing 130s for 25.0ml of remaining 1075.0ml @23:52:05.94 Intake.select :open @23:52:07.52 ATV.dial :extSrc @23:52:08.12 Intake @32.9psia, vacuum from 20.0 to 24.0psi @23:52:08.14 SS.reconfigure SSinhale.with maxSpeed: 32 @23:52:08.19 SS.seek 25ml @23:52:08.34 delay 3 seconds @23:52:11.37 ATV.dial :ElutionWater @23:52:12.44 AS.seek 0.12ml @23:52:18.77 ATV.dial :air3 @23:52:19.36 AS.seek 0.13ml @23:52:21.63 delay 2 seconds @23:52:23.65 ATV.dial :bleach @23:52:24.10 AS.seek 0.23ml @23:52:44.48 delay 3 seconds @23:52:47.51 ATV.dial :air3 @23:52:48.04 delay 3 seconds @23:52:51.13 AS.seek 0.255ml @23:52:56.44 delay 3 seconds @23:52:59.47 ATV.dial :bleach @23:53:00.06 AS.seek 0.24ml @23:53:03.19 Stroke completed -- waiting for pressure to equalize ... @23:53:03.35 delay 3 seconds @23:53:06.39 ATV.dial :ASV @23:53:07.48 ASV.dial :SPEdelivery @23:53:07.85 AFV.select :carrierSolution @23:53:07.96 delay 2 seconds @23:53:08.97 Intake.select :close @23:53:09.14 SSV.dial :exhaust @23:53:09.90 Exhaust.select :open @23:53:09.98 AS.reconfigure ASconfig @23:53:10.04 AS.seek 0.5ml @23:53:10.06 SS.reconfigure SSexhale @23:53:10.16 SS.seek 0ml @23:53:18.95 Exhaust.select :close @23:53:19.07 SSV.dial CBV @23:53:19.09 CBV.dial SSV @23:53:19.84 Allowing 130s for 25.0ml of remaining 1050.0ml @23:53:19.85 Intake.select :open @23:53:22.04 Intake @33.3psia, vacuum from 20.0 to 24.0psi @23:53:22.06 SS.reconfigure SSinhale.with maxSpeed: 32 @23:53:22.11 SS.seek 25ml @23:53:25.38 delay 1 second @23:53:26.40 AFV.select :holdingCoil @23:53:26.56 AS.reconfigure ASslow1 @23:53:26.61 AS.seek 0.17ml @23:54:17.11 Stroke completed -- waiting for pressure to equalize ... @23:54:24.11 Intake.select :close @23:54:24.27 SSV.dial :exhaust @23:54:25.03 Exhaust.select :open @23:54:25.15 SS.reconfigure SSexhale @23:54:25.20 SS.seek 0ml @23:54:33.24 SPE.hold 2:46:40,95C @23:54:33.30 delay 3:00 @23:54:34.08 Exhaust.select :close @23:54:34.20 SSV.dial CBV @23:54:34.22 CBV.dial SSV @23:54:35.02 Allowing 130s for 25.0ml of remaining 1025.0ml @23:54:35.03 Intake.select :open @23:54:37.22 Intake @32.9psia, vacuum from 20.0 to 24.0psi @23:54:37.24 SS.reconfigure SSinhale.with maxSpeed: 32 @23:54:37.29 SS.seek 25ml @23:55:32.31 Stroke completed -- waiting for pressure to equalize ... @23:55:39.36 Intake.select :close @23:55:39.52 SSV.dial :exhaust @23:55:40.28 Exhaust.select :open @23:55:40.40 SS.reconfigure SSexhale @23:55:40.45 SS.seek 0ml @23:55:49.37 Exhaust.select :close @23:55:49.49 SSV.dial CBV @23:55:49.51 CBV.dial SSV @23:55:50.29 Allowing 130s for 25.0ml of remaining 1000.0ml @23:55:50.30 Intake.select :open @23:55:52.48 Intake @33.1psia, vacuum from 20.0 to 24.0psi @23:55:52.50 SS.reconfigure SSinhale.with maxSpeed: 32 @23:55:52.55 SS.seek 25ml @23:56:32.96 Vacuum too high -- waiting up to 40s @23:56:34.91 Intake @32.8psia, vacuum from 20.0 to 24.0psi @23:56:34.93 SS.reconfigure SSinhale.with maxSpeed: 29 @23:56:34.97 SS.seek 25ml @23:56:51.22 Stroke completed -- waiting for pressure to equalize ... @23:56:57.05 Intake.select :close @23:56:57.21 SSV.dial :exhaust @23:56:57.98 Exhaust.select :open @23:56:58.09 SS.reconfigure SSexhale @23:56:58.15 SS.seek 0ml @23:57:07.09 Exhaust.select :close @23:57:07.21 SSV.dial CBV @23:57:07.23 CBV.dial SSV @23:57:08.01 Allowing 130s for 25.0ml of remaining 975.0ml @23:57:08.02 Intake.select :open @23:57:10.77 Intake @33.2psia, vacuum from 20.0 to 24.0psi @23:57:10.79 SS.reconfigure SSinhale.with maxSpeed: 29 @23:57:10.84 SS.seek 25ml @23:57:33.35 AFV.select :carrierSolution @23:57:33.45 delay 2 seconds @23:57:35.48 AS.reconfigure ASconfig @23:57:35.53 AS.seek 1ml @23:58:11.47 Stroke completed -- waiting for pressure to equalize ... @23:58:17.91 Intake.select :close @23:58:18.07 SSV.dial :exhaust @23:58:18.83 Exhaust.select :open @23:58:18.95 SS.reconfigure SSexhale @23:58:19.00 SS.seek 0ml @23:58:23.52 delay 1 second @23:58:24.54 AFV.select :holdingCoil @23:58:24.64 delay 1 second @23:58:25.67 ASV.dial :wasteD @23:58:26.63 AS.seek 0ml @23:58:27.83 Exhaust.select :close @23:58:27.89 BACfans.email "Started mfb in tube 2 at 20:52:30CEST10-Sep-19\n{1 => 6, 2 => 16}\nCan@20:48:26, 16.6C, 79% humidity, 14.6psia, 15.186V, 0.809A, 0.809A avg, 12.28W",Subject: "pcrBAC sampling at most 2000ml, wcr at most 2000ml" @23:58:27.92 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @23:58:27.94 Retry #10 of 10 after 10:00 @23:58:27.96 SSV.dial CBV @23:58:27.99 CBV.dial SSV @23:58:28.79 Allowing 130s for 25.0ml of remaining 950.0ml @23:58:28.81 Intake.select :open @23:58:30.99 Intake @33.1psia, vacuum from 20.0 to 24.0psi @23:58:31.01 SS.reconfigure SSinhale.with maxSpeed: 29 @23:58:31.06 SS.seek 25ml @23:59:24.40 Priming Eluate @23:59:24.41 AFV.select :carrierSolution @23:59:24.52 delay 2 seconds @23:59:26.56 AS.seek 0.5ml @23:59:31.97 Stroke completed -- waiting for pressure to equalize ... @23:59:38.40 Intake.select :close @23:59:38.56 SSV.dial :exhaust @23:59:39.33 Exhaust.select :open @23:59:39.45 SS.reconfigure SSexhale @23:59:39.50 SS.seek 0ml @23:59:48.59 Exhaust.select :close @23:59:48.71 SSV.dial CBV @23:59:48.73 CBV.dial SSV @23:59:49.51 Allowing 130s for 25.0ml of remaining 925.0ml @23:59:49.53 Intake.select :open @23:59:51.71 Intake @33.0psia, vacuum from 20.0 to 24.0psi @23:59:51.73 SS.reconfigure SSinhale.with maxSpeed: 29 @23:59:51.78 SS.seek 25ml @23:59:55.59 delay 1 second @23:59:56.61 AFV.select :holdingCoil @23:59:56.71 ATV.dial ASV @23:59:56.72 ASV.dial :SPErecovery @23:59:57.29 Swish #1 of 2 @23:59:57.37 AS.reconfigure ASslow0 @23:59:57.42 AS.seek 0.6ml @00:00:07.86CEST11-Sep-19 AS.seek 0.5ml @00:00:18.23 Swish #2 of 2 @00:00:18.32 AS.seek 0.6ml @00:00:28.80 AS.seek 0.5ml @00:00:39.16 delay 3 seconds @00:00:42.25 AS.reconfigure ASslow1 @00:00:42.30 AS.seek 0.684ml @00:00:52.09 Stroke completed -- waiting for pressure to equalize ... @00:00:58.55 Intake.select :close @00:00:58.71 SSV.dial :exhaust @00:00:59.47 Exhaust.select :open @00:00:59.60 SS.reconfigure SSexhale @00:00:59.65 SS.seek 0ml @00:01:08.43 Exhaust.select :close @00:01:08.55 SSV.dial CBV @00:01:08.57 CBV.dial SSV @00:01:09.34 Allowing 130s for 25.0ml of remaining 900.0ml @00:01:09.36 Intake.select :open @00:01:11.53 Intake @33.2psia, vacuum from 20.0 to 24.0psi @00:01:11.55 SS.reconfigure SSinhale.with maxSpeed: 29 @00:01:11.60 SS.seek 25ml @00:01:20.16 delay 3 seconds @00:01:23.19 Eluate primed @00:01:23.20 ATV.dial :waste2 @00:01:23.80 AS.reconfigure ASnorm @00:01:23.84 AS.seek 0ml @00:01:58.63 SPE.coast @00:01:58.71 ASV.dial :SPErecovery @00:01:58.73 Setting up PCR for Pp -- writing /var/log/esp/Pp19sep1100h2000ml.pcr @00:01:58.76 Building PCR Slug from ASV with MM1 mix, PP2 primer @00:01:58.77 Priming with carrier @00:01:58.78 ATV.dial ARV @00:01:59.70 ARV.dial :carrier @00:02:00.07 AS.seek 0.5ml @00:02:12.57 Stroke completed -- waiting for pressure to equalize ... @00:02:19.64 Intake.select :close @00:02:19.80 SSV.dial :exhaust @00:02:20.56 Exhaust.select :open @00:02:20.69 SS.reconfigure SSexhale @00:02:20.74 SS.seek 0ml @00:02:25.66 AFV.select :carrierSolution @00:02:25.77 delay 2 seconds @00:02:27.80 AS.reconfigure ASconfig @00:02:27.85 AS.seek 1ml @00:02:29.70 Exhaust.select :close @00:02:29.82 SSV.dial CBV @00:02:29.84 CBV.dial SSV @00:02:30.61 Allowing 130s for 25.0ml of remaining 875.0ml @00:02:30.63 Intake.select :open @00:02:32.82 Intake @33.2psia, vacuum from 20.0 to 24.0psi @00:02:32.84 SS.reconfigure SSinhale.with maxSpeed: 29 @00:02:32.89 SS.seek 25ml @00:02:56.90 delay 1 second @00:02:57.93 AFV.select :holdingCoil @00:02:58.03 delay 1 second @00:02:59.06 ATV.dial :waste @00:03:00.46 AS.reconfigure ASnorm @00:03:00.51 AS.seek :empty @00:03:22.82 Vacuum too high -- waiting up to 40s @00:03:24.77 Intake @32.9psia, vacuum from 20.0 to 24.0psi @00:03:24.79 SS.reconfigure SSinhale.with maxSpeed: 26 @00:03:24.84 SS.seek 25ml @00:03:36.86 Stroke completed -- waiting for pressure to equalize ... @00:03:42.66 Intake.select :close @00:03:42.83 SSV.dial :exhaust @00:03:43.59 Exhaust.select :open @00:03:43.71 SS.reconfigure SSexhale @00:03:43.76 SS.seek 0ml @00:03:51.24 AFV.select :carrierSolution @00:03:51.35 delay 2 seconds @00:03:52.67 Exhaust.select :close @00:03:52.78 SSV.dial CBV @00:03:52.81 CBV.dial SSV @00:03:53.38 AS.reconfigure ASconfig @00:03:53.43 AS.seek 0.5ml @00:03:53.57 Allowing 130s for 25.0ml of remaining 850.0ml @00:03:53.59 Intake.select :open @00:03:55.77 Intake @33.2psia, vacuum from 20.0 to 24.0psi @00:03:55.79 SS.reconfigure SSinhale.with maxSpeed: 26 @00:03:55.84 SS.seek 25ml @00:04:22.51 delay 1 second @00:04:23.53 AFV.select :holdingCoil @00:04:23.63 Building Slug @00:04:23.64 ATV.dial :ARV @00:04:25.16 delay 2 seconds @00:04:27.19 ARV.dial :air1 @00:04:27.57 delay 2 seconds @00:04:29.66 AS.reconfigure ASslow2 @00:04:29.71 AS.seek 0.506ml @00:04:35.93 delay 2 seconds @00:04:37.96 ARV.dial :MM1 @00:04:38.52 delay 2 seconds @00:04:40.55 Adding MM1 @00:04:40.63 AS.seek 0.524ml @00:04:58.91 delay 25 seconds @00:05:03.28 Stroke completed -- waiting for pressure to equalize ... @00:05:09.72 Intake.select :close @00:05:09.88 SSV.dial :exhaust @00:05:10.64 Exhaust.select :open @00:05:10.76 SS.reconfigure SSexhale @00:05:10.81 SS.seek 0ml @00:05:19.79 Exhaust.select :close @00:05:19.91 SSV.dial CBV @00:05:19.93 CBV.dial SSV @00:05:20.70 Allowing 130s for 25.0ml of remaining 825.0ml @00:05:20.72 Intake.select :open @00:05:22.90 Intake @33.2psia, vacuum from 20.0 to 24.0psi @00:05:22.92 SS.reconfigure SSinhale.with maxSpeed: 26 @00:05:22.96 SS.seek 25ml @00:05:23.94 ARV.dial :PP2 @00:05:24.32 Adding PP2 @00:05:24.34 delay 2 seconds @00:05:26.42 AS.seek 0.53ml @00:05:32.73 delay 10 seconds @00:05:42.76 ARV.dial :air2 @00:05:43.70 adding ARV air @00:05:43.71 delay 2 seconds @00:05:45.79 AS.seek 0.536ml @00:05:52.12 delay 5 seconds @00:05:57.15 ARV.dial :carrier @00:05:58.34 delay 7 seconds @00:06:05.44 AS.seek 0.594ml @00:06:30.46 Stroke completed -- waiting for pressure to equalize ... @00:06:37.54 Intake.select :close @00:06:37.70 SSV.dial :exhaust @00:06:38.46 Exhaust.select :open @00:06:38.58 SS.reconfigure SSexhale @00:06:38.63 SS.seek 0ml @00:06:47.39 Exhaust.select :close @00:06:47.51 SSV.dial CBV @00:06:47.53 CBV.dial SSV @00:06:48.32 Allowing 130s for 25.0ml of remaining 800.0ml @00:06:48.33 Intake.select :open @00:06:50.52 Intake @33.4psia, vacuum from 20.0 to 24.0psi @00:06:50.54 SS.reconfigure SSinhale.with maxSpeed: 26 @00:06:50.59 SS.seek 25ml @00:07:04.06 delay 5 seconds @00:07:09.09 ATV.dial :ASV @00:07:09.48 delay 2 seconds @00:07:11.57 AS.seek 0.6ml @00:07:17.98 delay 4 seconds @00:07:22.01 ATV.dial ARV @00:07:22.52 delay 2 seconds @00:07:24.55 ARV.dial :carrier @00:07:24.56 delay 2 seconds @00:07:26.66 AS.seek 0.65ml @00:07:58.29 Stroke completed -- waiting for pressure to equalize ... @00:08:05.98 Intake.select :close @00:08:06.15 SSV.dial :exhaust @00:08:06.92 Exhaust.select :open @00:08:07.03 SS.reconfigure SSexhale @00:08:07.09 SS.seek 0ml @00:08:15.85 Exhaust.select :close @00:08:15.97 SSV.dial CBV @00:08:15.99 CBV.dial SSV @00:08:16.76 Allowing 130s for 25.0ml of remaining 775.0ml @00:08:16.78 Intake.select :open @00:08:17.12 delay 4 seconds @00:08:18.96 Intake @33.3psia, vacuum from 20.0 to 24.0psi @00:08:18.98 SS.reconfigure SSinhale.with maxSpeed: 26 @00:08:19.03 SS.seek 25ml @00:08:21.15 ATV.dial PCR @00:08:21.73 ASV.dial :waste1 @00:08:22.48 ARV.dial :air1 @00:08:23.14 Positioning Slug by 0.324ml @00:08:23.23 AS.seek 0.326ml @00:08:27.96 BACfans.email "Started mfb in tube 2 at 20:52:30CEST10-Sep-19\n{1 => 6, 2 => 16}\nCan@20:48:26, 16.6C, 79% humidity, 14.6psia, 15.186V, 0.809A, 0.809A avg, 12.28W",Subject: "pcrBAC sampling at most 2000ml, wcr at most 2000ml" @00:08:27.99 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @00:09:26.59 Stroke completed -- waiting for pressure to equalize ... @00:09:34.28 Intake.select :close @00:09:34.44 SSV.dial :exhaust @00:09:35.20 Exhaust.select :open @00:09:35.32 SS.reconfigure SSexhale @00:09:35.37 SS.seek 0ml @00:09:44.29 Exhaust.select :close @00:09:44.41 SSV.dial CBV @00:09:44.43 CBV.dial SSV @00:09:45.18 Allowing 130s for 25.0ml of remaining 750.0ml @00:09:45.19 Intake.select :open @00:09:47.38 Intake @33.3psia, vacuum from 20.0 to 24.0psi @00:09:47.40 SS.reconfigure SSinhale.with maxSpeed: 26 @00:09:47.45 SS.seek 25ml @00:10:55.01 Stroke completed -- waiting for pressure to equalize ... @00:11:02.71 Intake.select :close @00:11:02.87 SSV.dial :exhaust @00:11:03.64 Exhaust.select :open @00:11:03.76 SS.reconfigure SSexhale @00:11:03.81 SS.seek 0ml @00:11:12.76 Exhaust.select :close @00:11:12.88 SSV.dial CBV @00:11:12.90 CBV.dial SSV @00:11:13.65 Allowing 130s for 25.0ml of remaining 725.0ml @00:11:13.66 Intake.select :open @00:11:15.85 Intake @33.4psia, vacuum from 20.0 to 24.0psi @00:11:15.87 SS.reconfigure SSinhale.with maxSpeed: 26 @00:11:15.92 SS.seek 25ml @00:12:09.51 Vacuum too high -- waiting up to 40s @00:12:11.39 Intake @33.1psia, vacuum from 20.0 to 24.0psi @00:12:11.41 SS.reconfigure SSinhale.with maxSpeed: 23 @00:12:11.46 SS.seek 25ml @00:12:27.31 Stroke completed -- waiting for pressure to equalize ... @00:12:33.70 Intake.select :close @00:12:33.86 SSV.dial :exhaust @00:12:34.62 Exhaust.select :open @00:12:34.75 SS.reconfigure SSexhale @00:12:34.80 SS.seek 0ml @00:12:43.57 Exhaust.select :close @00:12:43.69 SSV.dial CBV @00:12:43.71 CBV.dial SSV @00:12:44.48 Allowing 130s for 25.0ml of remaining 700.0ml @00:12:44.50 Intake.select :open @00:12:46.68 Intake @33.6psia, vacuum from 20.0 to 24.0psi @00:12:46.70 SS.reconfigure SSinhale.with maxSpeed: 23 @00:12:46.75 SS.seek 25ml @00:13:49.29 delay 5 seconds @00:13:54.32 ATV.dial ARV @00:13:55.04 ASV.dial :PCRlock @00:13:55.42 delay 5 seconds @00:14:00.45 Running Pp Profile60C350 thermocycling @00:14:00.46 PCR.program Profile60C350 @00:14:00.60 Profile60C350 should complete in < 1:48:05 @00:14:00.61 PCR.run 2:20:06.25 @00:14:02.91 Stroke completed -- waiting for pressure to equalize ... @00:14:10.01 Intake.select :close @00:14:10.18 SSV.dial :exhaust @00:14:10.95 Exhaust.select :open @00:14:11.07 SS.reconfigure SSexhale @00:14:11.12 SS.seek 0ml @00:14:20.13 Exhaust.select :close @00:14:20.25 SSV.dial CBV @00:14:20.27 CBV.dial SSV @00:14:21.03 Allowing 130s for 25.0ml of remaining 675.0ml @00:14:21.04 Intake.select :open @00:14:23.23 Intake @33.5psia, vacuum from 20.0 to 24.0psi @00:14:23.25 SS.reconfigure SSinhale.with maxSpeed: 23 @00:14:23.30 SS.seek 25ml @00:15:39.41 Stroke completed -- waiting for pressure to equalize ... @00:15:47.71 Intake.select :close @00:15:47.88 SSV.dial :exhaust @00:15:48.65 Exhaust.select :open @00:15:48.77 SS.reconfigure SSexhale @00:15:48.82 SS.seek 0ml @00:15:57.54 Exhaust.select :close @00:15:57.66 SSV.dial CBV @00:15:57.68 CBV.dial SSV @00:15:58.46 Allowing 130s for 25.0ml of remaining 650.0ml @00:15:58.48 Intake.select :open @00:16:00.66 Intake @33.5psia, vacuum from 20.0 to 24.0psi @00:16:00.68 SS.reconfigure SSinhale.with maxSpeed: 23 @00:16:00.73 SS.seek 25ml @00:17:17.23 Stroke completed -- waiting for pressure to equalize ... @00:17:24.95 Intake.select :close @00:17:25.11 SSV.dial :exhaust @00:17:25.87 Exhaust.select :open @00:17:25.99 SS.reconfigure SSexhale @00:17:26.04 SS.seek 0ml @00:17:34.99 Exhaust.select :close @00:17:35.11 SSV.dial CBV @00:17:35.13 CBV.dial SSV @00:17:35.88 Allowing 130s for 25.0ml of remaining 625.0ml @00:17:35.89 Intake.select :open @00:17:38.08 Intake @33.5psia, vacuum from 20.0 to 24.0psi @00:17:38.10 SS.reconfigure SSinhale.with maxSpeed: 23 @00:17:38.15 SS.seek 25ml @00:18:54.43 Stroke completed -- waiting for pressure to equalize ... @00:19:02.74 Intake.select :close @00:19:02.90 SSV.dial :exhaust @00:19:03.67 Exhaust.select :open @00:19:03.78 SS.reconfigure SSexhale @00:19:03.84 SS.seek 0ml @00:19:13.66 Exhaust.select :close @00:19:13.78 SSV.dial CBV @00:19:13.80 CBV.dial SSV @00:19:14.56 Allowing 130s for 25.0ml of remaining 600.0ml @00:19:14.58 Intake.select :open @00:19:16.75 Intake @33.5psia, vacuum from 20.0 to 24.0psi @00:19:16.78 SS.reconfigure SSinhale.with maxSpeed: 23 @00:19:16.82 SS.seek 25ml @00:20:32.71 Stroke completed -- waiting for pressure to equalize ... @00:20:41.06 Intake.select :close @00:20:41.22 SSV.dial :exhaust @00:20:41.98 Exhaust.select :open @00:20:42.10 SS.reconfigure SSexhale @00:20:42.15 SS.seek 0ml @00:20:51.20 Exhaust.select :close @00:20:51.32 SSV.dial CBV @00:20:51.34 CBV.dial SSV @00:20:52.12 Allowing 130s for 25.0ml of remaining 575.0ml @00:20:52.14 Intake.select :open @00:20:54.32 Intake @33.6psia, vacuum from 20.0 to 24.0psi @00:20:54.34 SS.reconfigure SSinhale.with maxSpeed: 23 @00:20:54.39 SS.seek 25ml @00:22:10.43 Stroke completed -- waiting for pressure to equalize ... @00:22:18.91 Intake.select :close @00:22:19.07 SSV.dial :exhaust @00:22:19.82 Exhaust.select :open @00:22:19.94 SS.reconfigure SSexhale @00:22:19.99 SS.seek 0ml @00:22:28.82 Exhaust.select :close @00:22:28.94 SSV.dial CBV @00:22:28.96 CBV.dial SSV @00:22:29.74 Allowing 130s for 25.0ml of remaining 549.9ml @00:22:29.75 Intake.select :open @00:22:31.93 Intake @33.6psia, vacuum from 20.0 to 24.0psi @00:22:31.95 SS.reconfigure SSinhale.with maxSpeed: 23 @00:22:32.00 SS.seek 25ml @00:23:38.57 Vacuum too high -- waiting up to 40s @00:23:40.72 Intake @33.3psia, vacuum from 20.0 to 24.0psi @00:23:40.74 SS.reconfigure SSinhale.with maxSpeed: 21 @00:23:40.79 SS.seek 25ml @00:23:51.34 Stroke completed -- waiting for pressure to equalize ... @00:23:59.05 Intake.select :close @00:23:59.21 SSV.dial :exhaust @00:23:59.98 Exhaust.select :open @00:24:00.12 SS.reconfigure SSexhale @00:24:00.17 SS.seek 0ml @00:24:09.13 Exhaust.select :close @00:24:09.25 SSV.dial CBV @00:24:09.27 CBV.dial SSV @00:24:10.04 Allowing 130s for 25.0ml of remaining 524.9ml @00:24:10.06 Intake.select :open @00:24:12.24 Intake @33.6psia, vacuum from 20.0 to 24.0psi @00:24:12.26 SS.reconfigure SSinhale.with maxSpeed: 21 @00:24:12.30 SS.seek 25ml @00:25:35.96 Stroke completed -- waiting for pressure to equalize ... @00:25:44.58 Intake.select :close @00:25:44.74 SSV.dial :exhaust @00:25:46.14 Exhaust.select :open @00:25:46.26 SS.reconfigure SSexhale @00:25:46.31 SS.seek 0ml @00:25:55.21 Exhaust.select :close @00:25:55.32 SSV.dial CBV @00:25:55.37 CBV.dial SSV @00:25:56.12 Allowing 130s for 25.0ml of remaining 499.9ml @00:25:56.14 Intake.select :open @00:25:58.32 Intake @33.5psia, vacuum from 20.0 to 24.0psi @00:25:58.34 SS.reconfigure SSinhale.with maxSpeed: 21 @00:25:58.39 SS.seek 25ml @00:27:21.76 Stroke completed -- waiting for pressure to equalize ... @00:27:30.75 Intake.select :close @00:27:30.91 SSV.dial :exhaust @00:27:31.68 Exhaust.select :open @00:27:31.80 SS.reconfigure SSexhale @00:27:31.86 SS.seek 0ml @00:27:40.65 Exhaust.select :close @00:27:40.77 SSV.dial CBV @00:27:40.82 CBV.dial SSV @00:27:41.55 Allowing 130s for 25.0ml of remaining 474.9ml @00:27:41.56 Intake.select :open @00:27:43.75 Intake @33.7psia, vacuum from 20.0 to 24.0psi @00:27:43.77 SS.reconfigure SSinhale.with maxSpeed: 21 @00:27:43.81 SS.seek 25ml @00:29:07.23 Stroke completed -- waiting for pressure to equalize ... @00:29:17.47 Intake.select :close @00:29:18.23 SSV.dial :exhaust @00:29:18.99 Exhaust.select :open @00:29:19.11 SS.reconfigure SSexhale @00:29:19.16 SS.seek 0ml @00:29:28.05 Exhaust.select :close @00:29:28.17 SSV.dial CBV @00:29:28.19 CBV.dial SSV @00:29:28.94 Allowing 130s for 25.0ml of remaining 449.9ml @00:29:28.96 Intake.select :open @00:29:31.14 Intake @33.7psia, vacuum from 20.0 to 24.0psi @00:29:31.16 SS.reconfigure SSinhale.with maxSpeed: 21 @00:29:31.22 SS.seek 25ml @00:30:54.58 Stroke completed -- waiting for pressure to equalize ... @00:31:04.83 Intake.select :close @00:31:04.99 SSV.dial :exhaust @00:31:05.76 Exhaust.select :open @00:31:05.88 SS.reconfigure SSexhale @00:31:05.93 SS.seek 0ml @00:31:14.93 Exhaust.select :close @00:31:15.05 SSV.dial CBV @00:31:15.07 CBV.dial SSV @00:31:15.83 Allowing 130s for 25.0ml of remaining 424.9ml @00:31:15.85 Intake.select :open @00:31:18.03 Intake @33.6psia, vacuum from 20.0 to 24.0psi @00:31:18.05 SS.reconfigure SSinhale.with maxSpeed: 21 @00:31:18.09 SS.seek 25ml @00:32:31.94 Vacuum too high -- waiting up to 40s @00:32:34.35 Intake @33.7psia, vacuum from 20.0 to 24.0psi @00:32:34.37 SS.reconfigure SSinhale.with maxSpeed: 19 @00:32:34.41 SS.seek 25ml @00:32:45.11 Stroke completed -- waiting for pressure to equalize ... @00:32:53.44 Intake.select :close @00:32:53.60 SSV.dial :exhaust @00:32:54.37 Exhaust.select :open @00:32:54.49 SS.reconfigure SSexhale @00:32:54.54 SS.seek 0ml @00:33:03.63 Exhaust.select :close @00:33:03.75 SSV.dial CBV @00:33:03.77 CBV.dial SSV @00:33:04.55 Allowing 130s for 25.0ml of remaining 399.9ml @00:33:04.57 Intake.select :open @00:33:06.75 Intake @33.7psia, vacuum from 20.0 to 24.0psi @00:33:06.77 SS.reconfigure SSinhale.with maxSpeed: 19 @00:33:06.82 SS.seek 25ml @00:34:38.71 Stroke completed -- waiting for pressure to equalize ... @00:34:48.94 Intake.select :close @00:34:49.10 SSV.dial :exhaust @00:34:49.87 Exhaust.select :open @00:34:49.99 SS.reconfigure SSexhale @00:34:50.04 SS.seek 0ml @00:34:59.33 Exhaust.select :close @00:34:59.45 SSV.dial CBV @00:34:59.47 CBV.dial SSV @00:35:00.23 Allowing 130s for 25.0ml of remaining 374.9ml @00:35:00.24 Intake.select :open @00:35:02.43 Intake @33.8psia, vacuum from 20.0 to 24.0psi @00:35:02.45 SS.reconfigure SSinhale.with maxSpeed: 19 @00:35:02.49 SS.seek 25ml @00:36:34.10 Stroke completed -- waiting for pressure to equalize ... @00:36:44.29 Intake.select :close @00:36:44.45 SSV.dial :exhaust @00:36:45.21 Exhaust.select :open @00:36:45.33 SS.reconfigure SSexhale @00:36:45.38 SS.seek 0ml @00:36:54.35 Exhaust.select :close @00:36:54.47 SSV.dial CBV @00:36:54.49 CBV.dial SSV @00:36:55.27 Allowing 130s for 25.0ml of remaining 349.9ml @00:36:55.29 Intake.select :open @00:36:57.46 Intake @33.9psia, vacuum from 20.0 to 24.0psi @00:36:57.48 SS.reconfigure SSinhale.with maxSpeed: 19 @00:36:57.54 SS.seek 25ml @00:38:29.58 Stroke completed -- waiting for pressure to equalize ... @00:38:40.42 Intake.select :close @00:38:40.59 SSV.dial :exhaust @00:38:41.35 Exhaust.select :open @00:38:41.47 SS.reconfigure SSexhale @00:38:41.52 SS.seek 0ml @00:38:50.47 Exhaust.select :close @00:38:50.59 SSV.dial CBV @00:38:50.61 CBV.dial SSV @00:38:51.38 Allowing 130s for 25.0ml of remaining 324.9ml @00:38:51.39 Intake.select :open @00:38:53.58 Intake @33.7psia, vacuum from 20.0 to 24.0psi @00:38:53.60 SS.reconfigure SSinhale.with maxSpeed: 19 @00:38:53.64 SS.seek 25ml @00:40:25.83 Stroke completed -- waiting for pressure to equalize ... @00:40:37.95 Intake.select :close @00:40:38.11 SSV.dial :exhaust @00:40:38.88 Exhaust.select :open @00:40:39.00 SS.reconfigure SSexhale @00:40:39.05 SS.seek 0ml @00:40:47.88 Exhaust.select :close @00:40:48.00 SSV.dial CBV @00:40:48.02 CBV.dial SSV @00:40:48.79 Allowing 130s for 25.0ml of remaining 299.9ml @00:40:48.80 Intake.select :open @00:40:50.99 Intake @33.9psia, vacuum from 20.0 to 24.0psi @00:40:51.01 SS.reconfigure SSinhale.with maxSpeed: 19 @00:40:51.06 SS.seek 25ml @00:42:12.88 Vacuum too high -- waiting up to 40s @00:42:15.26 Intake @33.6psia, vacuum from 20.0 to 24.0psi @00:42:15.28 SS.reconfigure SSinhale.with maxSpeed: 17 @00:42:15.32 SS.seek 25ml @00:42:27.02 Stroke completed -- waiting for pressure to equalize ... @00:42:37.90 Intake.select :close @00:42:38.07 SSV.dial :exhaust @00:42:38.83 Exhaust.select :open @00:42:38.95 SS.reconfigure SSexhale @00:42:39.00 SS.seek 0ml @00:42:48.21 Exhaust.select :close @00:42:48.33 SSV.dial CBV @00:42:48.35 CBV.dial SSV @00:42:49.13 Allowing 130s for 25.0ml of remaining 274.9ml @00:42:49.15 Intake.select :open @00:42:51.32 Intake @33.9psia, vacuum from 20.0 to 24.0psi @00:42:51.34 SS.reconfigure SSinhale.with maxSpeed: 17 @00:42:51.39 SS.seek 25ml @00:44:34.29 Stroke completed -- waiting for pressure to equalize ... @00:44:45.38 Intake.select :close @00:44:45.54 SSV.dial :exhaust @00:44:46.30 Exhaust.select :open @00:44:46.42 SS.reconfigure SSexhale @00:44:46.47 SS.seek 0ml @00:44:55.25 Exhaust.select :close @00:44:55.37 SSV.dial CBV @00:44:55.39 CBV.dial SSV @00:44:56.16 Allowing 130s for 25.0ml of remaining 249.9ml @00:44:56.17 Intake.select :open @00:44:58.36 Intake @34.0psia, vacuum from 20.0 to 24.0psi @00:44:58.38 SS.reconfigure SSinhale.with maxSpeed: 17 @00:44:58.42 SS.seek 25ml @00:46:41.36 Stroke completed -- waiting for pressure to equalize ... @00:46:54.11 Intake.select :close @00:46:54.27 SSV.dial :exhaust @00:46:55.02 Exhaust.select :open @00:46:55.14 SS.reconfigure SSexhale @00:46:55.19 SS.seek 0ml @00:47:03.96 Exhaust.select :close @00:47:04.08 SSV.dial CBV @00:47:04.10 CBV.dial SSV @00:47:04.88 Allowing 130s for 25.0ml of remaining 224.9ml @00:47:04.89 Intake.select :open @00:47:07.08 Intake @33.9psia, vacuum from 20.0 to 24.0psi @00:47:07.10 SS.reconfigure SSinhale.with maxSpeed: 17 @00:47:07.15 SS.seek 25ml @00:48:50.45 Stroke completed -- waiting for pressure to equalize ... @00:49:03.26 Intake.select :close @00:49:03.42 SSV.dial :exhaust @00:49:04.16 Exhaust.select :open @00:49:04.28 SS.reconfigure SSexhale @00:49:04.33 SS.seek 0ml @00:49:13.60 Exhaust.select :close @00:49:13.72 SSV.dial CBV @00:49:13.74 CBV.dial SSV @00:49:14.52 Allowing 130s for 25.0ml of remaining 199.9ml @00:49:14.53 Intake.select :open @00:49:17.32 Intake @33.9psia, vacuum from 20.0 to 24.0psi @00:49:17.34 SS.reconfigure SSinhale.with maxSpeed: 17 @00:49:17.39 SS.seek 25ml @00:51:00.19 Stroke completed -- waiting for pressure to equalize ... @00:51:13.10 Intake.select :close @00:51:13.27 SSV.dial :exhaust @00:51:14.05 Exhaust.select :open @00:51:14.17 SS.reconfigure SSexhale @00:51:14.22 SS.seek 0ml @00:51:23.22 Exhaust.select :close @00:51:23.34 SSV.dial CBV @00:51:23.36 CBV.dial SSV @00:51:24.14 Allowing 130s for 25.0ml of remaining 174.9ml @00:51:24.15 Intake.select :open @00:51:26.34 Intake @33.9psia, vacuum from 20.0 to 24.0psi @00:51:26.36 SS.reconfigure SSinhale.with maxSpeed: 17 @00:51:26.41 SS.seek 25ml @00:53:09.19 Stroke completed -- waiting for pressure to equalize ... @00:53:22.10 Intake.select :close @00:53:22.26 SSV.dial :exhaust @00:53:23.02 Exhaust.select :open @00:53:23.14 SS.reconfigure SSexhale @00:53:23.19 SS.seek 0ml @00:53:32.17 Exhaust.select :close @00:53:32.29 SSV.dial CBV @00:53:32.31 CBV.dial SSV @00:53:33.08 Allowing 130s for 25.0ml of remaining 149.9ml @00:53:33.09 Intake.select :open @00:53:35.28 Intake @34.0psia, vacuum from 20.0 to 24.0psi @00:53:35.29 SS.reconfigure SSinhale.with maxSpeed: 17 @00:53:35.34 SS.seek 25ml @00:54:29.44 Vacuum too high -- waiting up to 40s @00:54:31.87 Intake @33.7psia, vacuum from 20.0 to 24.0psi @00:54:31.89 SS.reconfigure SSinhale.with maxSpeed: 15 @00:54:31.94 SS.seek 25ml @00:55:27.71 Stroke completed -- waiting for pressure to equalize ... @00:55:41.16 Intake.select :close @00:55:41.32 SSV.dial :exhaust @00:55:42.06 Exhaust.select :open @00:55:42.18 SS.reconfigure SSexhale @00:55:42.23 SS.seek 0ml @00:55:51.19 Exhaust.select :close @00:55:51.31 SSV.dial CBV @00:55:51.94 CBV.dial SSV @00:55:52.72 Allowing 130s for 25.0ml of remaining 124.9ml @00:55:52.74 Intake.select :open @00:55:54.92 Intake @34.0psia, vacuum from 20.0 to 24.0psi @00:55:54.94 SS.reconfigure SSinhale.with maxSpeed: 15 @00:55:54.99 SS.seek 25ml @00:57:51.49 Stroke completed -- waiting for pressure to equalize ... @00:58:02.75 SS.reconfigure SSconfig @00:58:02.80 SS.hold @00:58:02.94 Intake.select :close @00:58:03.10 ShallowSampler::Clogged in WCR -- progress too slow after 1900.1ml @00:58:03.12 SSV.dial :exhaust @00:58:03.89 Exhaust.select :open @00:58:04.02 SS.reconfigure SSexhale @00:58:04.07 SS.seek 0ml @00:58:13.05 Exhaust.select :close @00:58:13.16 Sampled 1900.1ml @00:58:13.18 Drying puck before fixing for 4:00 @00:58:13.19 CBV.dial :waste2b @00:58:13.94 delay 1:00 @00:59:13.97 CSV.dial :air @00:59:14.42 CS.reconfigure CSfast @00:59:14.47 CS.seek 10ml @00:59:36.49 Pumping wcr puck from tube 2 up to 55psi... @00:59:36.51 CBV.dial SSV @00:59:37.84 CBV.dial between CSV and waste2b @00:59:38.82 CSV.dial between air and CTV @00:59:39.15 CS.seek 6.667ml @00:59:47.27 CTV.dial CSV,avoiding: :air @00:59:47.30 CSV.dial CTV @00:59:48.10 CS.reconfigure CSpressurize @00:59:48.15 CS.seek 0ml @01:00:05.83 CSV.dial :air @01:00:06.90 CS.reconfigure CSfast @01:00:06.95 CS.seek 10ml @01:00:29.12 CSV.dial between air and CTV @01:00:29.47 CS.seek 2.351ml @01:00:46.56 CTV.dial CSV,avoiding: :air @01:00:46.58 CSV.dial CTV @01:00:46.90 CS.reconfigure CSpressurize @01:00:46.95 CS.seek 0ml @01:00:50.16 CBV.dial :waste2b @01:00:50.50 CS.reconfigure CSconfig @01:00:50.55 CS.seek :empty @01:00:54.86 delay 1:00 @01:01:54.88 CTV.dial :air,avoiding: :intake @01:01:56.14 Fix #1 of 2 (with rnal) for 2:00 @01:01:56.16 CSV.dial CSR @01:01:56.67 Pulling 2ml of CSR.rnal @01:01:56.68 CSR.select :rnal @01:01:56.80 CS.seek 2ml @01:02:03.08 delay 5 seconds @01:02:08.11 CSR.select :air @01:02:08.23 CS.reconfigure CSfast @01:02:08.29 CS.seek 10ml @01:02:26.02 Pumping wcr puck from tube 2 up to 55psi... @01:02:26.04 CBV.dial SSV @01:02:27.38 CBV.dial between CSV and waste2b @01:02:28.34 CSV.dial between air and CTV @01:02:28.95 CS.seek 6.591ml @01:02:37.22 CTV.dial CSV,avoiding: :air @01:02:37.24 CSV.dial CTV @01:02:38.59 CS.reconfigure CSpressurize @01:02:38.64 CS.seek 0ml @01:02:55.51 delay 2:00 @01:04:55.54 CBV.dial :waste2b @01:04:55.88 CS.reconfigure CSconfig @01:04:55.93 CS.seek :empty @01:04:57.52 delay 1:00 @01:05:57.54 CTV.dial :air,avoiding: :intake @01:05:58.80 Fix #2 of 2 (with rnal) for 2:00 @01:05:58.82 CSV.dial CSR @01:05:59.33 Pulling 2ml of CSR.rnal @01:05:59.34 CSR.select :rnal @01:05:59.46 CS.seek 2ml @01:06:06.21 delay 5 seconds @01:06:11.24 CSR.select :air @01:06:11.37 CS.reconfigure CSfast @01:06:11.42 CS.seek 10ml @01:06:29.12 Pumping wcr puck from tube 2 up to 55psi... @01:06:29.14 CBV.dial SSV @01:06:30.44 CBV.dial between CSV and waste2b @01:06:31.50 CSV.dial between air and CTV @01:06:32.14 CS.seek 6.605ml @01:06:40.28 CTV.dial CSV,avoiding: :air @01:06:40.32 CSV.dial CTV @01:06:41.63 CS.reconfigure CSpressurize @01:06:41.68 CS.seek 0ml @01:06:58.32 delay 2:00 @01:08:58.35 CBV.dial :waste2b @01:08:58.68 CS.reconfigure CSconfig @01:08:58.73 CS.seek :empty @01:09:00.40 delay 1:00 @01:10:00.43 CTV.dial :air,avoiding: :intake @01:10:01.77 Storing wcr puck in Collection Clamp to tube 1 @01:10:01.96 SC.reconfigure SCtight @01:10:02.02 SC.seek 1 @01:10:04.15 SC.reconfigure SCloose @01:10:04.39 Elbow.reconfigure TightElbow @01:10:04.44 Elbow.seek 1 @01:10:06.28 Forearm.reconfigure TightForearm @01:10:06.33 Forearm.seek 1 + 700 counts @01:10:07.38 SE.reconfigure SEpick @01:10:07.46 SE.seek beamBreak @01:10:09.77 SE.configure SEconfig @01:10:09.94 Elbow.reconfigure LimpElbow @01:10:10.00 Forearm.reconfigure LooseForearm @01:10:10.05 Forearm.seek 1 @01:10:10.37 Elbow.hold @01:10:10.44 Elbow.reconfigure TightElbow @01:10:10.56 SE.seek 7.08 Pucks below Top Plate @01:10:11.01 Forearm.force -20 @01:10:11.06 Forearm.seek 1 + 329 counts @01:10:11.96 Forearm.reconfigure TightForearm @01:10:12.03 Forearm.seek 1 + 700 counts @01:10:12.34 SE.seek 8.08 Pucks below Top Plate @01:10:13.03 Forearm.reconfigure LooseForearm @01:10:13.08 Forearm.seek :retract @01:10:14.01 Elbow.seek CC @01:10:15.68 Elbow.reconfigure LooseElbow @01:10:15.80 Forearm.seek CC @01:10:16.86 Hand.close @01:10:17.53 CC.seek :open @01:10:22.07 Forearm.seek :retract @01:10:23.12 Elbow.reconfigure TightElbow @01:10:23.17 Elbow.seek 1 @01:10:25.08 Elbow.reconfigure LimpElbow @01:10:25.14 Forearm.seek 1 @01:10:26.17 Elbow.hold @01:10:26.25 Elbow.reconfigure TightElbow @01:10:26.30 Hand.open @01:10:27.06 Forearm.force -20 @01:10:27.10 Forearm.seek 1 + 345 counts @01:10:27.43 Forearm.reconfigure TightForearm @01:10:27.48 Forearm.seek 1 + 700 counts @01:10:27.91 SE.seek :down @01:10:29.07 Forearm.reconfigure LooseForearm @01:10:29.13 Forearm.seek :retract @01:10:29.94 SE.coast @01:10:30.08 SC.coast @01:10:30.13 Starting finalPurge @01:10:30.14 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @01:10:30.22 Hand.open @01:10:30.67 Elbow.seek FlushPuck::Garage @01:10:32.65 Elbow.reconfigure LooseElbow @01:10:32.76 Forearm.seek :garage @01:10:33.73 Hand.close @01:10:34.55 Forearm.seek :retract @01:10:35.56 Elbow.reconfigure TightElbow @01:10:35.61 Elbow.seek CC @01:10:38.50 Elbow.reconfigure LooseElbow @01:10:38.61 Forearm.reconfigure TightForearm @01:10:38.65 Forearm.seek CC @01:10:40.68 CC.seek :closed @01:10:44.94 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.66 @01:10:45.01 Hand.open @01:10:45.73 Forearm.reconfigure LooseForearm @01:10:45.78 Forearm.seek :retract @01:10:46.81 Flushing the Collection Valve::Manifold @01:10:46.96 CSV.dial CSR @01:10:47.45 Pulling 5ml of CSR.flush CSR.select :flush @01:10:47.58 CS.seek 5ml @01:11:01.34 delay 5 seconds @01:11:06.37 CSR.select :air @01:11:06.55 CS.reconfigure CSfast @01:11:06.60 CS.seek 7.5ml @01:11:12.93 CSV.dial :waste @01:11:14.15 CS.reconfigure CSconfig @01:11:14.20 CS.seek 0ml @01:11:34.43 Flushing lower manifold's waste1 port @01:11:34.57 CSV.dial :flush @01:11:35.04 CS.reconfigure CSfast @01:11:35.09 CS.seek 5ml @01:11:46.68 delay 5 seconds @01:11:51.70 CSV.dial :air @01:11:52.22 CS.seek 7ml @01:11:57.62 CSV.dial CTV @01:11:57.66
CTV.dial CSV @01:11:59.01 CBV.dial :waste1 @01:11:59.79 CS.seek 0ml @01:12:15.44 Flushing lower manifold's waste2b port @01:12:15.58 CSV.dial :flush @01:12:16.43 CS.seek 5ml @01:12:27.90 delay 5 seconds @01:12:32.92 CSV.dial :air @01:12:33.46 CS.seek 7ml @01:12:38.78 CSV.dial CTV @01:12:38.81
CTV.dial CSV @01:12:39.26 CBV.dial :waste2b @01:12:40.02 CS.seek 0ml @01:12:55.53 Flushing upper manifold @01:12:55.67 CSV.dial :flush @01:12:56.40 CS.seek 5ml @01:13:07.96 delay 5 seconds @01:13:12.98 CSV.dial :air @01:13:13.50 CS.seek 7ml @01:13:18.84 CSV.dial CBV @01:13:18.88
CBV.dial CSV @01:13:19.93 CTV.dial :air @01:13:21.19 CS.seek 0ml @01:13:36.84 delay 7 seconds @01:13:43.87 CS.seek 3ml @01:13:51.40 CSV.dial :waste @01:13:52.57 CS.seek 0ml @01:14:00.00 CSV.dial :flush @01:14:00.47 CS.seek 3ml @01:14:07.90 delay 5 seconds @01:14:12.92 CSV.dial :air @01:14:13.43 CS.seek 5ml @01:14:18.87 CSV.dial :air @01:14:18.89 CS.seek 0ml @01:14:30.34 delay 7 seconds @01:14:37.37 CS.seek 3ml @01:14:44.75 CSV.dial :waste @01:14:45.48 CS.seek 0ml @01:14:52.81 Disinfecting external Intake with mfbkill @01:14:52.95 CSV.dial CSR @01:14:54.27 Pulling 8ml of CSR.mfbkill CSR.select :mfbkill @01:14:54.40 CS.seek 8ml @01:15:12.15 delay 5 seconds @01:15:17.18 CSR.select :air @01:15:17.30 CS.seek 10ml @01:15:22.59 CSV.dial CBV @01:15:22.66
CBV.dial CSV @01:15:23.70 CTV.dial :intake @01:15:24.43 Intake.select :open @01:15:24.55 CS.seek 2ml @01:15:43.28 Intake.select :close @01:15:43.39 CTV.dial :air @01:15:44.14 CS.reconfigure CSconfig @01:15:44.19 CS.seek 0ml @01:15:50.34 Flushing lower manifold's waste1 port @01:15:50.35 CSV.dial CSR @01:15:51.39 Pulling 4ml of CSR.mfbkill CSR.select :mfbkill @01:15:51.52 CS.seek 4ml @01:16:02.75 delay 5 seconds @01:16:07.77 CSR.select :air @01:16:07.89 CS.reconfigure CSfast @01:16:07.94 CS.seek 10ml @01:16:21.40 CSV.dial CTV @01:16:21.43
CTV.dial CSV @01:16:22.78 CBV.dial :waste1 @01:16:23.26 CS.seek 0ml @01:16:44.87 Flushing lower manifold's waste2b port @01:16:44.88 CSV.dial CSR @01:16:45.39 Pulling 4ml of CSR.mfbkill @01:16:45.40 CSR.select :mfbkill @01:16:45.53 CS.reconfigure CSconfig @01:16:45.58 CS.seek 4ml @01:16:56.84 delay 5 seconds @01:17:01.86 CSR.select :air @01:17:01.98 CS.reconfigure CSfast @01:17:02.03 CS.seek 10ml @01:17:15.53 CSV.dial CTV @01:17:15.56
CTV.dial CSV @01:17:16.03 CBV.dial :waste2b @01:17:16.79 CS.seek 0ml @01:17:38.47 Killing upper manifold @01:17:38.48 CSV.dial CSR @01:17:39.00 Pulling 4ml of CSR.mfbkill @01:17:39.01 CSR.select :mfbkill @01:17:39.14 CS.reconfigure CSconfig @01:17:39.18 CS.seek 4ml @01:17:51.06 delay 5 seconds @01:17:56.08 CSR.select :air @01:17:56.21 CS.reconfigure CSfast @01:17:56.25 CS.seek 10ml @01:18:09.72 CSV.dial CBV @01:18:09.75
CBV.dial CSV @01:18:10.80 CTV.dial :air @01:18:12.07 CS.seek 0ml @01:18:33.72 delay 7 seconds @01:18:40.75 CS.seek 3ml @01:18:48.12 CSV.dial :waste @01:18:48.57 CS.seek 0ml @01:18:56.28 Flushing upper manifold @01:18:56.42 CSV.dial :flush @01:18:56.88 CS.seek 5ml @01:19:08.44 delay 5 seconds @01:19:13.46 CSV.dial :air @01:19:14.10 CS.seek 7.001ml @01:19:19.37 CSV.dial CBV @01:19:19.40
CBV.dial CSV @01:19:20.39 CTV.dial :air @01:19:20.41 CS.seek 0ml @01:19:35.94 delay 7 seconds @01:19:42.97 CS.seek 3ml @01:19:50.25 CSV.dial :waste @01:19:50.70 CS.seek 0ml @01:19:58.32 CSV.dial :flush @01:19:58.78 CS.seek 3ml @01:20:06.03 delay 5 seconds @01:20:11.05 CSV.dial :air @01:20:11.55 CS.seek 5ml @01:20:16.88 CSV.dial :air @01:20:16.90 CS.seek 0ml @01:20:28.31 delay 7 seconds @01:20:35.34 CS.seek 3ml @01:20:42.84 CSV.dial :waste @01:20:43.56 CS.seek 0ml @01:20:50.78 Flushing lower manifold's waste1 port @01:20:50.92 CSV.dial :flush @01:20:51.49 CS.seek 5ml @01:21:03.13 delay 5 seconds @01:21:08.15 CSV.dial :air @01:21:08.70 CS.seek 7ml @01:21:13.97 CSV.dial CTV @01:21:14.00
CTV.dial CSV @01:21:15.36 CBV.dial :waste1 @01:21:15.84 CS.seek 0ml @01:21:31.31 Flushing lower manifold's waste2b port @01:21:31.45 CSV.dial :flush @01:21:32.18 CS.seek 5ml @01:21:43.56 delay 5 seconds @01:21:48.58 CSV.dial :air @01:21:49.12 CS.seek 7ml @01:21:54.36 CSV.dial CTV @01:21:54.39
CTV.dial CSV @01:21:55.42 CBV.dial :waste2b @01:21:56.18 CS.seek 0ml @01:22:11.66 Disinfecting external Intake with kill @01:22:11.80 CSV.dial CSR @01:22:12.31 Pulling 8ml of CSR.kill @01:22:12.32 CSR.select :kill @01:22:12.44 CS.seek 8ml @01:22:30.19 delay 5 seconds @01:22:35.21 CSR.select :air @01:22:35.34 CS.seek 10ml @01:22:40.78 CSV.dial CBV @01:22:40.81
CBV.dial CSV @01:22:41.89 CTV.dial :intake @01:22:42.61 Intake.select :open @01:22:42.73 CS.seek 2ml @01:23:01.19 Intake.select :close @01:23:01.30 CTV.dial :air @01:23:02.05 CS.reconfigure CSconfig @01:23:02.10 CS.seek 0ml @01:23:08.41 CSV.dial CSR @01:23:09.45 Pulling 5ml of CSR.flush @01:23:09.46 CSR.select :flush @01:23:09.59 CS.seek 5ml @01:23:23.53 delay 5 seconds @01:23:28.56 CSR.select :air @01:23:28.68 CS.seek 10ml @01:23:42.44 CSV.dial CBV @01:23:43.42 CS.seek 0ml @01:24:09.94 Garaging FlushPuck in Collection Clamp @01:24:10.03 Elbow.reconfigure TightElbow @01:24:10.07 Elbow.seek CC @01:24:10.34 Elbow.reconfigure LooseElbow @01:24:10.44 Forearm.seek CC @01:24:11.49 Hand.close @01:24:12.17 CC.seek :open @01:24:16.52 Forearm.seek :retract @01:24:17.56 Elbow.reconfigure TightElbow @01:24:17.61 Elbow.seek :garage @01:24:20.77 Elbow.reconfigure LooseElbow @01:24:20.87 Forearm.reconfigure TightForearm @01:24:20.92 Forearm.seek :garage @01:24:22.11 Hand.open @01:24:22.74 Forearm.reconfigure LooseForearm @01:24:22.79 Forearm.seek :retract @01:24:23.80 Completed [mbaPhase] begun 20:48:26 @01:24:23.81 Preparing to sleep until 20:45 11-september-19 @01:24:23.86 deferred until MFBPCR finish @01:24:23.88
Waiting for MFBPCR til 20:45 11-september-19 @01:24:23.91 Continuing at 20:45 @01:24:23.97 GoodNews.email "Then, in tube 2: mbaPhase\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:125\n{1 => 9, 2 => 13}\nCan@01:24:23, 17.6C, 79% humidity, 14.6psia, 14.807V, 0.613A, 0.607A avg, 9.08W",Subject: "Waiting until 20:45" @01:24:24.01 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @01:24:24.02 Retry #1 of 10 after 10:00 @01:34:24.04 GoodNews.email "Then, in tube 2: mbaPhase\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:125\n{1 => 9, 2 => 13}\nCan@01:24:23, 17.6C, 79% humidity, 14.6psia, 14.807V, 0.613A, 0.607A avg, 9.08W",Subject: "Waiting until 20:45" @01:34:24.07 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @01:34:24.08 Retry #2 of 10 after 10:00 @01:37:21.91 PCR.off @01:37:23.96 PCR for Pp completed -- closed /var/log/esp/Pp19sep1100h2000ml.pcr @01:37:24.04 Cleaning PCR @01:37:24.05 AFV.select :carrierSolution @01:37:24.16 delay 2 seconds @01:37:26.19 AS.reconfigure ASconfig @01:37:26.24 AS.seek 0.5ml @01:37:36.57 delay 1 second @01:37:37.59 AFV.select :holdingCoil @01:37:37.69 ASV.dial :waste1 @01:37:38.07 ATV.dial :air3 @01:37:39.28 AS.reconfigure ASslow1 @01:37:39.33 AS.seek 0.51ml @01:37:41.61 delay 2 seconds @01:37:43.63 ATV.dial :bleach @01:37:44.23 AS.seek 0.81ml @01:38:44.83 delay 3 seconds @01:38:47.86 ATV.dial :air3 @01:38:48.38 delay 3 seconds @01:38:51.47 AS.seek 0.835ml @01:38:57.35 delay 3 seconds @01:39:00.38 ATV.dial :bleach @01:39:00.98 AS.seek 0.82ml @01:39:04.29 delay 3 seconds @01:39:07.32 ARV.dial :waste @01:39:07.91 delay 3 seconds @01:39:10.94 ATV.dial :ARV @01:39:11.79 AS.reconfigure ASconfig @01:39:11.84 AS.seek 0.47ml @01:39:32.25 AFV.select :carrierSolution @01:39:32.36 delay 2 seconds @01:39:34.40 AS.seek 1ml @01:40:05.14 delay 1 second @01:40:06.16 AFV.select :holdingCoil @01:40:06.26 delay 1 second @01:40:07.29 AS.reconfigure ASfast @01:40:07.34 AS.seek 0ml @01:40:34.68 AFV.select :carrierSolution @01:40:34.79 delay 2 seconds @01:40:36.82 AS.reconfigure ASconfig @01:40:36.87 AS.seek 1ml @01:41:34.66 delay 1 second @01:41:35.69 AFV.select :holdingCoil @01:41:35.79 delay 1 second @01:41:36.82 AS.reconfigure ASfast @01:41:36.87 AS.seek 0ml @01:42:04.22 AFV.select :carrierSolution @01:42:04.33 delay 2 seconds @01:42:06.36 AS.reconfigure ASconfig @01:42:06.41 AS.seek 1ml @01:43:04.16 delay 1 second @01:43:05.18 AFV.select :holdingCoil @01:43:05.28 delay 1 second @01:43:06.31 AS.reconfigure ASfast @01:43:06.36 AS.seek 0ml @01:43:33.72 ARV.dial between carrier and air1 @01:43:34.21 ATV.dial :air3 @01:43:35.42 AS.reconfigure ASslow1 @01:43:35.47 AS.seek 0.01ml @01:43:37.86 delay 2 seconds @01:43:39.89 ATV.dial :bleach @01:43:40.50 AS.seek 0.81ml @01:44:24.11 GoodNews.email "Then, in tube 2: mbaPhase\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:125\n{1 => 9, 2 => 13}\nCan@01:24:23, 17.6C, 79% humidity, 14.6psia, 14.807V, 0.613A, 0.607A avg, 9.08W",Subject: "Waiting until 20:45" @01:44:24.14 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @01:44:24.16 Retry #3 of 10 after 10:00 @01:46:21.69 delay 3 seconds @01:46:24.72 ATV.dial :air3 @01:46:25.24 delay 3 seconds @01:46:28.33 AS.seek 0.835ml @01:46:33.61 delay 3 seconds @01:46:36.64 ATV.dial :bleach @01:46:37.23 AS.seek 0.82ml @01:46:40.54 delay 3 seconds @01:46:43.57 ATV.dial PCR @01:46:43.96 AS.reconfigure ASconfig @01:46:44.01 AS.seek :empty @01:47:31.46 PCR.program :bleachProfile @01:47:31.51 PCR program should complete in < 2:45 @01:47:31.52 PCR.run 10:00 @01:50:36.31 PCR.off @01:50:36.34 AFV.select :carrierSolution @01:50:36.45 delay 2 seconds @01:50:38.49 AS.seek 1ml @01:51:36.30 delay 1 second @01:51:37.32 AFV.select :holdingCoil @01:51:37.42 delay 1 second @01:51:38.45 AS.reconfigure ASfast @01:51:38.50 AS.seek 0ml @01:52:05.85 delay 3 seconds @01:52:08.89 AFV.select :carrierSolution @01:52:08.99 delay 2 seconds @01:52:11.02 AS.reconfigure ASconfig @01:52:11.07 AS.seek 1ml @01:53:08.85 delay 1 second @01:53:09.87 AFV.select :holdingCoil @01:53:09.97 delay 1 second @01:53:11.00 AS.reconfigure ASfast @01:53:11.05 AS.seek 0ml @01:53:38.40 delay 3 seconds @01:53:41.43 AFV.select :carrierSolution @01:53:41.53 delay 2 seconds @01:53:43.57 AS.reconfigure ASconfig @01:53:43.61 AS.seek 1ml @01:54:24.18 GoodNews.email "Then, in tube 2: mbaPhase\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:125\n{1 => 9, 2 => 13}\nCan@01:24:23, 17.6C, 79% humidity, 14.6psia, 14.807V, 0.613A, 0.607A avg, 9.08W",Subject: "Waiting until 20:45" @01:54:24.21 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @01:54:24.22 Retry #4 of 10 after 10:00 @01:54:41.35 delay 1 second @01:54:42.37 AFV.select :holdingCoil @01:54:42.47 delay 1 second @01:54:43.50 AS.reconfigure ASfast @01:54:43.55 AS.seek 0ml @01:55:10.93 delay 3 seconds @01:55:13.97 AFV.select :carrierSolution @01:55:14.07 delay 2 seconds @01:55:16.10 AS.reconfigure ASconfig @01:55:16.16 AS.seek 1ml @01:56:13.94 delay 1 second @01:56:14.96 AFV.select :holdingCoil @01:56:15.06 delay 1 second @01:56:16.59 AS.reconfigure ASfast @01:56:16.72 AS.seek 0ml @01:56:44.05 delay 3 seconds @01:56:47.08 AFV.select :carrierSolution @01:56:47.18 delay 2 seconds @01:56:49.21 AS.reconfigure ASconfig @01:56:49.27 AS.seek 1ml @01:57:47.02 delay 1 second @01:57:48.04 AFV.select :holdingCoil @01:57:48.14 delay 1 second @01:57:49.17 AS.reconfigure ASnorm @01:57:49.22 AS.seek 0ml @01:58:39.93 Setting up PCR for Mm -- writing /var/log/esp/Mm19sep1101h2000ml.pcr @01:58:39.95 Building PCR Slug from ASV with MM2 mix, PP3 primer @01:58:39.96 Priming with carrier @01:58:39.97 ATV.dial ARV @01:58:40.65 ARV.dial :carrier @01:58:41.01 AS.seek 0.5ml @01:59:06.63 AFV.select :carrierSolution @01:59:06.74 delay 2 seconds @01:59:08.77 AS.reconfigure ASconfig @01:59:08.82 AS.seek 1ml @01:59:37.88 delay 1 second @01:59:38.91 AFV.select :holdingCoil @01:59:39.01 delay 1 second @01:59:40.04 ATV.dial :waste @01:59:41.57 AS.reconfigure ASnorm @01:59:41.62 AS.seek :empty @02:00:32.37 AFV.select :carrierSolution @02:00:32.48 delay 2 seconds @02:00:34.51 AS.reconfigure ASconfig @02:00:34.56 AS.seek 0.5ml @02:01:03.63 delay 1 second @02:01:04.65 AFV.select :holdingCoil @02:01:04.75 Building Slug @02:01:04.76 ATV.dial :ARV @02:01:06.29 delay 2 seconds @02:01:08.32 ARV.dial :air1 @02:01:08.70 delay 2 seconds @02:01:10.79 AS.reconfigure ASslow2 @02:01:10.84 AS.seek 0.506ml @02:01:17.02 delay 2 seconds @02:01:19.05 ARV.dial :MM2 @02:01:20.43 delay 2 seconds @02:01:22.46 Adding MM2 @02:01:22.55 AS.seek 0.524ml @02:01:40.79 delay 25 seconds @02:02:05.82 ARV.dial :PP3 @02:02:06.20 Adding PP3 @02:02:06.22 delay 2 seconds @02:02:08.31 AS.seek 0.53ml @02:02:14.49 delay 10 seconds @02:02:24.52 ARV.dial :air2 @02:02:24.88 adding ARV air @02:02:24.90 delay 2 seconds @02:02:26.98 AS.seek 0.536ml @02:02:33.24 delay 5 seconds @02:02:38.27 ARV.dial :carrier @02:02:39.45 delay 7 seconds @02:02:46.58 AS.seek 0.594ml @02:03:45.07 delay 5 seconds @02:03:50.10 ATV.dial :ASV @02:03:50.49 delay 2 seconds @02:03:52.58 AS.seek 0.6ml @02:03:58.79 delay 4 seconds @02:04:02.82 ATV.dial ARV @02:04:03.34 delay 2 seconds @02:04:05.37 ARV.dial :carrier @02:04:05.38 delay 2 seconds @02:04:07.47 AS.seek 0.65ml @02:04:24.25 GoodNews.email "Then, in tube 2: mbaPhase\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:125\n{1 => 9, 2 => 13}\nCan@01:24:23, 17.6C, 79% humidity, 14.6psia, 14.807V, 0.613A, 0.607A avg, 9.08W",Subject: "Waiting until 20:45" @02:04:24.28 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @02:04:24.30 Retry #5 of 10 after 10:00 @02:04:57.96 delay 4 seconds @02:05:01.99 ATV.dial PCR @02:05:02.55 ASV.dial :waste1 @02:05:02.56 ARV.dial :air1 @02:05:02.95 Positioning Slug by 0.324ml @02:05:03.05 AS.seek 0.326ml @02:10:29.16 delay 5 seconds @02:10:34.19 ATV.dial ARV @02:10:34.90 ASV.dial :PCRlock @02:10:35.29 delay 5 seconds @02:10:40.32 Running Mm Profile60C350 thermocycling @02:10:40.33 PCR.program Profile60C350 @02:10:40.48 Profile60C350 should complete in < 1:48:05 @02:10:40.49 PCR.run 2:20:06.25 @02:14:24.32 GoodNews.email "Then, in tube 2: mbaPhase\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:125\n{1 => 9, 2 => 13}\nCan@01:24:23, 17.6C, 79% humidity, 14.6psia, 14.807V, 0.613A, 0.607A avg, 9.08W",Subject: "Waiting until 20:45" @02:14:24.35 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @02:14:24.37 Retry #6 of 10 after 10:00 @02:24:24.39 GoodNews.email "Then, in tube 2: mbaPhase\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:125\n{1 => 9, 2 => 13}\nCan@01:24:23, 17.6C, 79% humidity, 14.6psia, 14.807V, 0.613A, 0.607A avg, 9.08W",Subject: "Waiting until 20:45" @02:24:24.42 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @02:24:24.43 Retry #7 of 10 after 10:00 @02:34:24.46 GoodNews.email "Then, in tube 2: mbaPhase\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:125\n{1 => 9, 2 => 13}\nCan@01:24:23, 17.6C, 79% humidity, 14.6psia, 14.807V, 0.613A, 0.607A avg, 9.08W",Subject: "Waiting until 20:45" @02:34:24.49 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @02:34:24.51 Retry #8 of 10 after 10:00 @02:44:24.53 GoodNews.email "Then, in tube 2: mbaPhase\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:125\n{1 => 9, 2 => 13}\nCan@01:24:23, 17.6C, 79% humidity, 14.6psia, 14.807V, 0.613A, 0.607A avg, 9.08W",Subject: "Waiting until 20:45" @02:44:24.56 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @02:44:24.58 Retry #9 of 10 after 10:00 @02:54:24.60 GoodNews.email "Then, in tube 2: mbaPhase\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:125\n{1 => 9, 2 => 13}\nCan@01:24:23, 17.6C, 79% humidity, 14.6psia, 14.807V, 0.613A, 0.607A avg, 9.08W",Subject: "Waiting until 20:45" @02:54:24.63 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @02:54:25.26 Retry #10 of 10 after 10:00 @03:04:25.28 GoodNews.email "Then, in tube 2: mbaPhase\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:125\n{1 => 9, 2 => 13}\nCan@01:24:23, 17.6C, 79% humidity, 14.6psia, 14.807V, 0.613A, 0.607A avg, 9.08W",Subject: "Waiting until 20:45" @03:04:25.31 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @03:33:47.76 PCR.off @03:33:50.27 PCR for Mm completed -- closed /var/log/esp/Mm19sep1101h2000ml.pcr @03:33:50.34 Cleaning PCR @03:33:50.36 AFV.select :carrierSolution @03:33:50.47 delay 2 seconds @03:33:52.50 AS.reconfigure ASconfig @03:33:52.55 AS.seek 0.5ml @03:34:02.88 delay 1 second @03:34:03.90 AFV.select :holdingCoil @03:34:04.00 ASV.dial :waste1 @03:34:04.38 ATV.dial :air3 @03:34:05.59 AS.reconfigure ASslow1 @03:34:05.64 AS.seek 0.51ml @03:34:07.92 delay 2 seconds @03:34:09.94 ATV.dial :bleach @03:34:10.55 AS.seek 0.81ml @03:35:11.15 delay 3 seconds @03:35:14.18 ATV.dial :air3 @03:35:14.69 delay 3 seconds @03:35:17.78 AS.seek 0.835ml @03:35:23.08 delay 3 seconds @03:35:26.11 ATV.dial :bleach @03:35:26.70 AS.seek 0.82ml @03:35:29.99 delay 3 seconds @03:35:33.02 ARV.dial :waste @03:35:33.60 delay 3 seconds @03:35:36.63 ATV.dial :ARV @03:35:37.48 AS.reconfigure ASconfig @03:35:37.53 AS.seek 0.47ml @03:35:57.90 AFV.select :carrierSolution @03:35:58.02 delay 2 seconds @03:36:00.06 AS.seek 1ml @03:36:30.85 delay 1 second @03:36:31.87 AFV.select :holdingCoil @03:36:31.97 delay 1 second @03:36:33.00 AS.reconfigure ASfast @03:36:33.05 AS.seek 0ml @03:37:00.40 AFV.select :carrierSolution @03:37:00.52 delay 2 seconds @03:37:02.55 AS.reconfigure ASconfig @03:37:02.60 AS.seek 1ml @03:38:00.44 delay 1 second @03:38:01.46 AFV.select :holdingCoil @03:38:01.56 delay 1 second @03:38:02.59 AS.reconfigure ASfast @03:38:02.64 AS.seek 0ml @03:38:29.99 AFV.select :carrierSolution @03:38:30.10 delay 2 seconds @03:38:32.13 AS.reconfigure ASconfig @03:38:32.18 AS.seek 1ml @03:39:29.96 delay 1 second @03:39:30.98 AFV.select :holdingCoil @03:39:31.08 delay 1 second @03:39:32.11 AS.reconfigure ASfast @03:39:32.16 AS.seek 0ml @03:39:59.51 ARV.dial between carrier and air1 @03:39:59.99 ATV.dial :air3 @03:40:01.20 AS.reconfigure ASslow1 @03:40:01.25 AS.seek 0.01ml @03:40:03.57 delay 2 seconds @03:40:05.59 ATV.dial :bleach @03:40:06.20 AS.seek 0.81ml @03:42:47.34 delay 3 seconds @03:42:50.37 ATV.dial :air3 @03:42:50.90 delay 3 seconds @03:42:53.99 AS.seek 0.835ml @03:42:59.30 delay 3 seconds @03:43:02.33 ATV.dial :bleach @03:43:02.94 AS.seek 0.82ml @03:43:06.23 delay 3 seconds @03:43:09.26 ATV.dial PCR @03:43:09.63 AS.reconfigure ASconfig @03:43:09.68 AS.seek :empty @03:43:57.15 PCR.program :bleachProfile @03:43:57.19 PCR program should complete in < 2:45 @03:43:57.21 PCR.run 10:00 @03:47:02.02 PCR.off @03:47:02.05 AFV.select :carrierSolution @03:47:02.16 delay 2 seconds @03:47:04.21 AS.seek 1ml @03:48:02.04 delay 1 second @03:48:03.06 AFV.select :holdingCoil @03:48:03.16 delay 1 second @03:48:04.19 AS.reconfigure ASfast @03:48:04.24 AS.seek 0ml @03:48:31.59 delay 3 seconds @03:48:34.62 AFV.select :carrierSolution @03:48:34.72 delay 2 seconds @03:48:36.75 AS.reconfigure ASconfig @03:48:36.80 AS.seek 1ml @03:49:34.62 delay 1 second @03:49:35.64 AFV.select :holdingCoil @03:49:35.74 delay 1 second @03:49:36.77 AS.reconfigure ASfast @03:49:36.82 AS.seek 0ml @03:50:04.18 delay 3 seconds @03:50:07.21 AFV.select :carrierSolution @03:50:07.31 delay 2 seconds @03:50:09.34 AS.reconfigure ASconfig @03:50:09.40 AS.seek 1ml @03:51:07.20 delay 1 second @03:51:08.22 AFV.select :holdingCoil @03:51:08.32 delay 1 second @03:51:09.35 AS.reconfigure ASfast @03:51:09.40 AS.seek 0ml @03:51:36.74 delay 3 seconds @03:51:39.77 AFV.select :carrierSolution @03:51:39.87 delay 2 seconds @03:51:41.90 AS.reconfigure ASconfig @03:51:41.95 AS.seek 1ml @03:52:39.73 delay 1 second @03:52:40.75 AFV.select :holdingCoil @03:52:40.85 delay 1 second @03:52:41.88 AS.reconfigure ASfast @03:52:41.93 AS.seek 0ml @03:53:09.28 delay 3 seconds @03:53:12.31 AFV.select :carrierSolution @03:53:12.41 delay 2 seconds @03:53:14.44 AS.reconfigure ASconfig @03:53:14.49 AS.seek 1ml @03:54:12.27 delay 1 second @03:54:13.29 AFV.select :holdingCoil @03:54:13.39 delay 1 second @03:54:14.42 AS.reconfigure ASnorm @03:54:14.47 AS.seek 0ml @03:55:05.19 Setting up PCR for Ss -- writing /var/log/esp/Ss19sep1103h2000ml.pcr @03:55:05.20 Building PCR Slug from ASV with MM2 mix, PP4 primer @03:55:05.22 Priming with carrier @03:55:05.23 ATV.dial ARV @03:55:05.82 ARV.dial :carrier @03:55:06.16 AS.seek 0.5ml @03:55:31.68 AFV.select :carrierSolution @03:55:31.79 delay 2 seconds @03:55:33.82 AS.reconfigure ASconfig @03:55:33.87 AS.seek 1ml @03:56:02.90 delay 1 second @03:56:03.93 AFV.select :holdingCoil @03:56:04.03 delay 1 second @03:56:05.06 ATV.dial :waste @03:56:06.60 AS.reconfigure ASnorm @03:56:06.65 AS.seek :empty @03:56:57.39 AFV.select :carrierSolution @03:56:57.50 delay 2 seconds @03:56:59.53 AS.reconfigure ASconfig @03:56:59.58 AS.seek 0.5ml @03:57:28.68 delay 1 second @03:57:29.70 AFV.select :holdingCoil @03:57:29.80 Building Slug @03:57:29.81 ATV.dial :ARV @03:57:31.34 delay 2 seconds @03:57:33.37 ARV.dial :air1 @03:57:33.75 delay 2 seconds @03:57:35.84 AS.reconfigure ASslow2 @03:57:35.89 AS.seek 0.506ml @03:57:42.10 delay 2 seconds @03:57:44.12 ARV.dial :MM2 @03:57:45.48 delay 2 seconds @03:57:47.51 Adding MM2 @03:57:47.59 AS.seek 0.524ml @03:58:05.90 delay 25 seconds @03:58:30.93 ARV.dial :PP4 @03:58:31.29 Adding PP4 @03:58:31.30 delay 2 seconds @03:58:33.39 AS.seek 0.53ml @03:58:39.59 delay 10 seconds @03:58:49.62 ARV.dial :air3 @03:58:49.99 adding ARV air @03:58:50.01 delay 2 seconds @03:58:52.09 AS.seek 0.536ml @03:58:58.51 delay 5 seconds @03:59:03.54 ARV.dial :carrier @03:59:04.71 delay 7 seconds @03:59:11.80 AS.seek 0.594ml @04:00:10.56 delay 5 seconds @04:00:15.59 ATV.dial :ASV @04:00:15.98 delay 2 seconds @04:00:18.07 AS.seek 0.6ml @04:00:24.48 delay 4 seconds @04:00:28.51 ATV.dial ARV @04:00:29.03 delay 2 seconds @04:00:31.06 ARV.dial :carrier @04:00:31.07 delay 2 seconds @04:00:33.16 AS.seek 0.65ml @04:01:23.76 delay 4 seconds @04:01:27.79 ATV.dial PCR @04:01:28.35 ASV.dial :waste1 @04:01:28.36 ARV.dial :air1 @04:01:29.32 Positioning Slug by 0.324ml @04:01:29.41 AS.seek 0.326ml @04:06:55.39 delay 5 seconds @04:07:00.42 ATV.dial ARV @04:07:01.14 ASV.dial :PCRlock @04:07:01.53 delay 5 seconds @04:07:06.56 Running Ss Profile60C350 thermocycling @04:07:06.57 PCR.program Profile60C350 @04:07:06.71 Profile60C350 should complete in < 1:48:05 @04:07:06.72 PCR.run 2:20:06.25 @05:30:07.43 PCR.off @05:30:09.59 PCR for Ss completed -- closed /var/log/esp/Ss19sep1103h2000ml.pcr @05:30:09.66 Cleaning PCR @05:30:09.68 AFV.select :carrierSolution @05:30:09.79 delay 2 seconds @05:30:11.82 AS.reconfigure ASconfig @05:30:11.87 AS.seek 0.5ml @05:30:22.18 delay 1 second @05:30:23.20 AFV.select :holdingCoil @05:30:23.30 ASV.dial :waste1 @05:30:23.68 ATV.dial :air3 @05:30:24.89 AS.reconfigure ASslow1 @05:30:24.94 AS.seek 0.51ml @05:30:27.13 delay 2 seconds @05:30:29.15 ATV.dial :bleach @05:30:29.75 AS.seek 0.81ml @05:31:30.37 delay 3 seconds @05:31:33.40 ATV.dial :air3 @05:31:33.92 delay 3 seconds @05:31:37.01 AS.seek 0.835ml @05:31:42.33 delay 3 seconds @05:31:45.36 ATV.dial :bleach @05:31:45.97 AS.seek 0.82ml @05:31:49.26 delay 3 seconds @05:31:52.29 ARV.dial :waste @05:31:52.85 delay 3 seconds @05:31:55.88 ATV.dial :ARV @05:31:56.72 AS.reconfigure ASconfig @05:31:56.77 AS.seek 0.47ml @05:32:17.18 AFV.select :carrierSolution @05:32:17.29 delay 2 seconds @05:32:19.33 AS.seek 1ml @05:32:50.12 delay 1 second @05:32:51.14 AFV.select :holdingCoil @05:32:51.24 delay 1 second @05:32:52.27 AS.reconfigure ASfast @05:32:52.32 AS.seek 0ml @05:33:19.66 AFV.select :carrierSolution @05:33:19.78 delay 2 seconds @05:33:21.81 AS.reconfigure ASconfig @05:33:21.86 AS.seek 1ml @05:34:19.70 delay 1 second @05:34:20.72 AFV.select :holdingCoil @05:34:20.82 delay 1 second @05:34:21.84 AS.reconfigure ASfast @05:34:21.89 AS.seek 0ml @05:34:49.23 AFV.select :carrierSolution @05:34:49.35 delay 2 seconds @05:34:51.38 AS.reconfigure ASconfig @05:34:51.43 AS.seek 1ml @05:35:49.24 delay 1 second @05:35:50.26 AFV.select :holdingCoil @05:35:50.36 delay 1 second @05:35:51.39 AS.reconfigure ASfast @05:35:51.44 AS.seek 0ml @05:36:18.79 ARV.dial between carrier and air1 @05:36:19.26 ATV.dial :air3 @05:36:20.47 AS.reconfigure ASslow1 @05:36:20.52 AS.seek 0.01ml @05:36:22.84 delay 2 seconds @05:36:24.86 ATV.dial :bleach @05:36:25.47 AS.seek 0.81ml @05:39:06.64 delay 3 seconds @05:39:09.67 ATV.dial :air3 @05:39:10.20 delay 3 seconds @05:39:13.29 AS.seek 0.835ml @05:39:18.58 delay 3 seconds @05:39:21.61 ATV.dial :bleach @05:39:22.22 AS.seek 0.82ml @05:39:25.46 delay 3 seconds @05:39:28.49 ATV.dial PCR @05:39:28.87 AS.reconfigure ASconfig @05:39:28.92 AS.seek :empty @05:40:16.39 PCR.program :bleachProfile @05:40:16.44 PCR program should complete in < 2:45 @05:40:16.45 PCR.run 10:00 @05:43:21.88 PCR.off @05:43:21.92 AFV.select :carrierSolution @05:43:22.03 delay 2 seconds @05:43:24.07 AS.seek 1ml @05:44:21.90 delay 1 second @05:44:22.92 AFV.select :holdingCoil @05:44:23.02 delay 1 second @05:44:24.05 AS.reconfigure ASfast @05:44:24.10 AS.seek 0ml @05:44:51.43 delay 3 seconds @05:44:54.46 AFV.select :carrierSolution @05:44:54.56 delay 2 seconds @05:44:56.59 AS.reconfigure ASconfig @05:44:56.64 AS.seek 1ml @05:45:54.42 delay 1 second @05:45:55.44 AFV.select :holdingCoil @05:45:55.54 delay 1 second @05:45:56.57 AS.reconfigure ASfast @05:45:56.62 AS.seek 0ml @05:46:23.97 delay 3 seconds @05:46:27.00 AFV.select :carrierSolution @05:46:27.10 delay 2 seconds @05:46:29.13 AS.reconfigure ASconfig @05:46:29.19 AS.seek 1ml @05:47:26.98 delay 1 second @05:47:28.00 AFV.select :holdingCoil @05:47:28.10 delay 1 second @05:47:29.13 AS.reconfigure ASfast @05:47:29.18 AS.seek 0ml @05:47:56.54 delay 3 seconds @05:47:59.57 AFV.select :carrierSolution @05:47:59.67 delay 2 seconds @05:48:01.70 AS.reconfigure ASconfig @05:48:01.75 AS.seek 1ml @05:48:59.54 delay 1 second @05:49:00.56 AFV.select :holdingCoil @05:49:00.66 delay 1 second @05:49:01.69 AS.reconfigure ASfast @05:49:01.74 AS.seek 0ml @05:49:29.09 delay 3 seconds @05:49:32.12 AFV.select :carrierSolution @05:49:32.22 delay 2 seconds @05:49:34.25 AS.reconfigure ASconfig @05:49:34.31 AS.seek 1ml @05:50:32.14 delay 1 second @05:50:33.16 AFV.select :holdingCoil @05:50:33.26 delay 1 second @05:50:34.29 AS.reconfigure ASnorm @05:50:34.34 AS.seek 0ml @05:51:25.07 Setting up PCR for IPc -- writing /var/log/esp/IPc19sep1105h2000ml.pcr @05:51:25.09 Building PCR Slug from ASV with MM1 mix, PP1 primer @05:51:25.11 Priming with carrier @05:51:25.12 ATV.dial ARV @05:51:25.70 ARV.dial :carrier @05:51:26.05 AS.seek 0.5ml @05:51:51.61 AFV.select :carrierSolution @05:51:51.72 delay 2 seconds @05:51:53.75 AS.reconfigure ASconfig @05:51:53.80 AS.seek 1ml @05:52:22.81 delay 1 second @05:52:23.83 AFV.select :holdingCoil @05:52:23.93 delay 1 second @05:52:24.96 ATV.dial :waste @05:52:26.50 AS.reconfigure ASnorm @05:52:26.55 AS.seek :empty @05:53:17.29 AFV.select :carrierSolution @05:53:17.40 delay 2 seconds @05:53:19.43 AS.reconfigure ASconfig @05:53:19.48 AS.seek 0.5ml @05:53:48.59 delay 1 second @05:53:49.61 AFV.select :holdingCoil @05:53:49.72 Building Slug @05:53:49.73 ATV.dial :ARV @05:53:51.26 delay 2 seconds @05:53:53.29 ARV.dial :air1 @05:53:53.68 delay 2 seconds @05:53:55.77 AS.reconfigure ASslow2 @05:53:55.82 AS.seek 0.506ml @05:54:02.03 delay 2 seconds @05:54:04.06 ARV.dial :MM1 @05:54:04.62 delay 2 seconds @05:54:06.65 Adding MM1 @05:54:06.72 AS.seek 0.524ml @05:54:24.97 delay 25 seconds @05:54:50.00 ARV.dial :PP1 @05:54:50.39 Adding PP1 @05:54:50.41 delay 2 seconds @05:54:52.49 AS.seek 0.53ml @05:54:58.70 delay 10 seconds @05:55:08.73 ARV.dial :air1 @05:55:09.09 adding ARV air @05:55:09.10 delay 2 seconds @05:55:11.19 AS.seek 0.536ml @05:55:17.47 delay 5 seconds @05:55:22.50 ARV.dial :carrier @05:55:22.86 delay 7 seconds @05:55:29.95 AS.seek 0.594ml @05:56:28.45 delay 5 seconds @05:56:33.48 ATV.dial :ASV @05:56:33.87 delay 2 seconds @05:56:35.96 AS.seek 0.6ml @05:56:42.20 delay 4 seconds @05:56:46.23 ATV.dial ARV @05:56:46.75 delay 2 seconds @05:56:48.78 ARV.dial :carrier @05:56:48.79 delay 2 seconds @05:56:50.88 AS.seek 0.65ml @05:57:41.34 delay 4 seconds @05:57:45.37 ATV.dial PCR @05:57:46.51 ASV.dial :waste1 @05:57:46.55 ARV.dial :air1 @05:57:46.94 Positioning Slug by 0.324ml @05:57:47.03 AS.seek 0.326ml @06:03:13.11 delay 5 seconds @06:03:18.14 ATV.dial ARV @06:03:18.86 ASV.dial :PCRlock @06:03:19.25 delay 5 seconds @06:03:24.28 Running IPc ProfileIPc thermocycling @06:03:24.29 PCR.program ProfileIPc @06:03:24.44 ProfileIPc should complete in < 1:50:49 @06:03:24.45 PCR.run 2:23:31.25 @07:28:04.03 PCR.off @07:28:06.74 PCR for IPc completed -- closed /var/log/esp/IPc19sep1105h2000ml.pcr @07:28:06.75 Full MFB Decontamination @07:28:06.76 Decon extraction column and decon AIV @07:28:06.79 SPE.hold 2:46:40,55C @07:28:06.84 ASV.dial :SPEdelivery @07:28:07.43 delay 20 seconds @07:28:27.46 AFV.select :carrierSolution @07:28:27.56 delay 2 seconds @07:28:29.59 AS.reconfigure ASconfig @07:28:29.64 AS.seek 0.5ml @07:28:39.95 delay 1 second @07:28:40.97 AFV.select :holdingCoil @07:28:41.07 ATV.dial :air1 @07:28:42.74 AS.reconfigure ASslow1 @07:28:42.79 AS.seek 0.52ml @07:28:47.08 ATV.dial :ASV @07:28:48.62 ASV.dial :SPErecovery @07:28:49.24 AS.seek 0.62ml @07:29:09.61 ATV.dial :waste @07:29:11.36 AS.reconfigure ASnorm @07:29:11.41 AS.seek :empty @07:29:43.04 AFV.select :holdingCoil @07:29:43.05 ATV.dial :waste @07:29:43.07 AS.reconfigure ASconfig @07:29:43.12 AS.seek 0ml @07:29:43.41 AFV.select :carrierSolution @07:29:43.53 delay 2 seconds @07:29:45.57 AS.seek 0.5ml @07:30:14.59 delay 1 second @07:30:15.61 AFV.select :holdingCoil @07:30:15.71 ATV.dial :air3 @07:30:16.36 AS.reconfigure ASslow1 @07:30:16.41 AS.seek 0.52ml @07:30:20.73 ATV.dial :air3 @07:30:20.82 AS.seek 0.53ml @07:30:23.08 delay 2 seconds @07:30:25.10 ATV.dial :bleach @07:30:25.55 AS.seek 0.71ml @07:31:02.03 delay 3 seconds @07:31:05.06 ATV.dial :air3 @07:31:05.58 delay 3 seconds @07:31:08.67 AS.seek 0.735ml @07:31:13.99 delay 3 seconds @07:31:17.02 ATV.dial :bleach @07:31:17.61 AS.seek 0.72ml @07:31:20.92 delay 3 seconds @07:31:23.95 ATV.dial ASV @07:31:24.92 ASV.dial :SPErecovery @07:31:25.01 AS.seek 0.365ml @07:32:36.64 ATV.dial :waste @07:32:38.23 AS.reconfigure ASnorm @07:32:38.28 AS.seek :empty @07:32:57.62 ATV.dial :air3 @07:32:58.26 AS.reconfigure ASslow1 @07:32:58.31 AS.seek 0.009ml @07:33:00.64 delay 2 seconds @07:33:02.66 ATV.dial :bleach @07:33:03.12 AS.seek 0.579ml @07:34:58.01 delay 3 seconds @07:35:01.04 ATV.dial :air3 @07:35:01.56 delay 3 seconds @07:35:04.65 AS.seek 0.604ml @07:35:09.98 delay 3 seconds @07:35:13.01 ATV.dial :bleach @07:35:13.60 AS.seek 0.589ml @07:35:16.92 delay 3 seconds @07:35:19.95 ATV.dial :ASV @07:35:21.01 ASV.dial :wasteR @07:35:21.38 AS.seek 0ml @07:37:20.14 delay 1:00 @07:38:20.17 ATV.dial ASV @07:38:20.18 AFV.select :carrierSolution @07:38:20.28 delay 2 seconds @07:38:22.31 AS.reconfigure ASconfig @07:38:22.36 AS.seek 1ml @07:39:20.11 delay 1 second @07:39:21.13 AFV.select :holdingCoil @07:39:21.23 delay 1 second @07:39:22.26 ASV.dial :wasteR @07:39:22.28 AS.reconfigure ASslow1 @07:39:22.32 AS.seek 0ml @07:42:43.69 delay 10 seconds @07:42:53.72 ATV.dial :air3 @07:42:55.12 AS.seek 0.01ml @07:42:57.45 delay 2 seconds @07:42:59.47 ATV.dial :bleach @07:43:00.07 AS.seek 0.58ml @07:44:54.97 delay 3 seconds @07:44:58.00 ATV.dial :air3 @07:44:58.52 delay 3 seconds @07:45:01.61 AS.seek 0.605ml @07:45:06.90 delay 3 seconds @07:45:09.93 ATV.dial :bleach @07:45:10.52 AS.seek 0.59ml @07:45:13.83 delay 3 seconds @07:45:16.86 ATV.dial ASV @07:45:17.81 ASV.dial :SPEdelivery @07:45:18.17 AS.seek 0ml @07:47:17.06 delay 1:00 @07:48:17.09 AFV.select :carrierSolution @07:48:17.19 delay 2 seconds @07:48:19.22 AS.reconfigure ASconfig @07:48:19.27 AS.seek 1ml @07:49:17.08 delay 1 second @07:49:18.10 AFV.select :holdingCoil @07:49:18.20 delay 1 second @07:49:19.23 ATV.dial ASV @07:49:19.24 ASV.dial :SPEdelivery @07:49:19.26 AS.reconfigure ASslow1 @07:49:19.30 AS.seek 0ml @07:52:40.69 delay 10 seconds @07:52:50.72 ATV.dial :air2 @07:52:51.50 AS.reconfigure ASslow0 @07:52:51.55 AS.seek 0.8ml @07:54:12.35 delay 3 seconds @07:54:15.39 ATV.dial :ASV @07:54:16.32 ASV.dial :SPEdelivery @07:54:16.34 AS.reconfigure ASslow1 @07:54:16.39 AS.seek 0.03ml @07:56:51.52 delay 20 seconds @07:57:11.55 ATV.dial :waste,via: :air3 @07:57:13.13 AS.reconfigure ASconfig @07:57:13.18 AS.seek :empty @07:57:15.26 SPE.coast @07:57:15.35 Removing Bleach from Mixing Coil @07:57:15.37 AFV.select :carrierSolution @07:57:15.47 delay 2 seconds @07:57:17.51 AS.seek 0.5ml @07:57:46.63 delay 1 second @07:57:47.65 AFV.select :holdingCoil @07:57:47.75 ATV.dial :ASV @07:57:49.33 ASV.dial :SPErecovery @07:57:49.96 AS.reconfigure ASslow1 @07:57:50.01 AS.seek 0.73ml @07:58:36.52 ASV.dial :wasteD @07:58:37.09 AS.reconfigure ASnorm @07:58:37.14 AS.seek 0ml @07:59:14.24 AFV.select :carrierSolution @07:59:14.35 delay 2 seconds @07:59:16.38 AS.reconfigure ASconfig @07:59:16.43 AS.seek 0.5ml @07:59:45.45 delay 1 second @07:59:46.47 AFV.select :holdingCoil @07:59:46.57 ATV.dial ASV @07:59:46.58 ASV.dial :SPErecovery @07:59:47.79 AS.reconfigure ASslow0 @07:59:47.86 AS.seek 0.3ml @08:00:08.30 ATV.dial :air2 @08:00:09.31 AS.reconfigure ASslow1 @08:00:09.36 AS.seek 0.32ml @08:00:13.67 ATV.dial ASV @08:00:14.67 AS.reconfigure ASslow0 @08:00:14.72 AS.seek 0.57ml @08:00:40.19 ASV.dial :wasteD @08:00:40.76 AS.reconfigure ASnorm @08:00:40.81 AS.seek 0ml @08:01:09.89 AFV.select :carrierSolution @08:01:10.00 delay 2 seconds @08:01:12.03 AS.reconfigure ASconfig @08:01:12.08 AS.seek 0.5ml @08:01:41.10 delay 1 second @08:01:42.12 AFV.select :holdingCoil @08:01:42.22 ATV.dial ASV @08:01:42.23 ASV.dial :SPErecovery @08:01:42.85 AS.reconfigure ASslow0 @08:01:42.90 AS.seek 0.3ml @08:02:03.33 ATV.dial :air2 @08:02:04.34 AS.reconfigure ASslow1 @08:02:04.39 AS.seek 0.32ml @08:02:08.72 ATV.dial ASV @08:02:09.73 AS.reconfigure ASslow0 @08:02:09.78 AS.seek 0.57ml @08:02:35.25 ASV.dial :wasteD @08:02:35.89 AS.reconfigure ASnorm @08:02:35.95 AS.seek 0ml @08:03:05.03 ATV.dial :air2 @08:03:05.97 AS.reconfigure ASslow0 @08:03:06.02 AS.seek 0.3ml @08:03:36.55 ATV.dial :ASV @08:03:37.47 ASV.dial :wasteD @08:03:37.49 AS.reconfigure ASconfig @08:03:37.54 AS.seek 0.05ml @08:03:52.22 ATV.dial :waste @08:03:53.97 AS.seek 0ml @08:03:57.16 ASV.dial :SPEdelivery @08:03:58.08 deconLysateINT @08:03:58.09 ATV.dial :air3 @08:03:58.74 AS.reconfigure ASslow1 @08:03:58.79 AS.seek 0.01ml @08:04:01.14 delay 2 seconds @08:04:03.16 ATV.dial :bleach @08:04:03.62 AS.seek 0.96ml @08:07:14.99 delay 3 seconds @08:07:18.02 ATV.dial :air3 @08:07:18.53 delay 3 seconds @08:07:21.62 AS.seek 0.985ml @08:07:26.96 delay 3 seconds @08:07:29.99 ATV.dial :bleach @08:07:30.59 AS.seek 0.97ml @08:07:33.89 delay 3 seconds @08:07:36.92 ATV.dial AIV @08:07:38.10 AIV.dial :coilToMFB @08:07:38.11 AWV.select :waste @08:07:38.13 AFV.select :carrierSolution @08:07:38.24 delay 2 seconds @08:07:40.27 AS.reconfigure ASconfig @08:07:40.32 AS.seek 1ml @08:07:42.39 delay 1 second @08:07:43.41 AFV.select :holdingCoil @08:07:43.51 delay 1 second @08:07:44.54 AS.reconfigure ASslow0 @08:07:44.59 AS.seek :empty @08:09:25.55 AFV.select :carrierSolution @08:09:25.66 delay 2 seconds @08:09:27.69 AS.reconfigure ASconfig @08:09:27.74 AS.seek 1ml @08:10:25.53 delay 1 second @08:10:26.55 AFV.select :holdingCoil @08:10:26.65 delay 1 second @08:10:27.68 AS.reconfigure ASslow0 @08:10:27.72 AS.seek :empty @08:12:08.71 AFV.select :carrierSolution @08:12:08.82 delay 2 seconds @08:12:10.85 AS.reconfigure ASconfig @08:12:10.90 AS.seek 1ml @08:13:08.71 delay 1 second @08:13:09.73 AFV.select :holdingCoil @08:13:09.83 delay 1 second @08:13:10.86 AS.reconfigure ASslow0 @08:13:10.91 AS.seek :empty @08:14:51.86 ATV.dial :air2 @08:14:52.51 AS.reconfigure ASslow1 @08:14:52.56 AS.seek 0.009ml @08:14:54.85 AS.reconfigure ASslow0 @08:14:54.91 AS.seek 1ml @08:16:35.44 ATV.dial AIV @08:16:36.26 AS.seek 0.02ml @08:18:15.11 ATV.dial :waste @08:18:16.64 AS.seek 0ml @08:18:18.94 AWV.select :air @08:18:19.10 Cleaning PCR @08:18:19.12 AFV.select :carrierSolution @08:18:19.22 delay 2 seconds @08:18:21.25 AS.reconfigure ASconfig @08:18:21.30 AS.seek 0.5ml @08:18:50.39 delay 1 second @08:18:51.41 AFV.select :holdingCoil @08:18:51.51 ASV.dial :waste1 @08:18:51.88 ATV.dial :air3 @08:18:52.54 AS.reconfigure ASslow1 @08:18:52.59 AS.seek 0.51ml @08:18:54.88 delay 2 seconds @08:18:56.90 ATV.dial :bleach @08:18:57.36 AS.seek 0.81ml @08:19:57.97 delay 3 seconds @08:20:01.00 ATV.dial :air3 @08:20:01.52 delay 3 seconds @08:20:04.61 AS.seek 0.835ml @08:20:09.89 delay 3 seconds @08:20:12.92 ATV.dial :bleach @08:20:13.51 AS.seek 0.82ml @08:20:16.82 delay 3 seconds @08:20:19.85 ARV.dial :waste @08:20:20.41 delay 3 seconds @08:20:23.44 ATV.dial :ARV @08:20:24.28 AS.reconfigure ASconfig @08:20:24.33 AS.seek 0.47ml @08:20:44.75 AFV.select :carrierSolution @08:20:44.86 delay 2 seconds @08:20:46.90 AS.seek 1ml @08:21:17.69 delay 1 second @08:21:18.71 AFV.select :holdingCoil @08:21:18.81 delay 1 second @08:21:19.84 AS.reconfigure ASfast @08:21:19.89 AS.seek 0ml @08:21:47.24 AFV.select :carrierSolution @08:21:47.35 delay 2 seconds @08:21:49.38 AS.reconfigure ASconfig @08:21:49.43 AS.seek 1ml @08:22:47.19 delay 1 second @08:22:48.21 AFV.select :holdingCoil @08:22:48.31 delay 1 second @08:22:49.34 AS.reconfigure ASfast @08:22:49.39 AS.seek 0ml @08:23:16.74 AFV.select :carrierSolution @08:23:16.85 delay 2 seconds @08:23:18.88 AS.reconfigure ASconfig @08:23:18.93 AS.seek 1ml @08:24:16.69 delay 1 second @08:24:17.71 AFV.select :holdingCoil @08:24:17.81 delay 1 second @08:24:18.84 AS.reconfigure ASfast @08:24:18.89 AS.seek 0ml @08:24:46.22 ARV.dial between carrier and air1 @08:24:46.68 ATV.dial :air3 @08:24:47.89 AS.reconfigure ASslow1 @08:24:47.94 AS.seek 0.01ml @08:24:50.29 delay 2 seconds @08:24:52.32 ATV.dial :bleach @08:24:52.90 AS.seek 0.81ml @08:27:34.04 delay 3 seconds @08:27:37.07 ATV.dial :air3 @08:27:37.58 delay 3 seconds @08:27:40.67 AS.seek 0.835ml @08:27:45.98 delay 3 seconds @08:27:49.01 ATV.dial :bleach @08:27:49.61 AS.seek 0.82ml @08:27:52.93 delay 3 seconds @08:27:55.96 ATV.dial PCR @08:27:56.33 AS.reconfigure ASconfig @08:27:56.38 AS.seek :empty @08:28:43.85 PCR.program :bleachProfile @08:28:43.90 PCR program should complete in < 2:45 @08:28:43.91 PCR.run 10:00 @08:32:00.87 PCR.off @08:32:01.51 AFV.select :carrierSolution @08:32:01.62 delay 2 seconds @08:32:03.66 AS.seek 1ml @08:33:01.48 delay 1 second @08:33:02.50 AFV.select :holdingCoil @08:33:02.60 delay 1 second @08:33:03.63 AS.reconfigure ASfast @08:33:03.68 AS.seek 0ml @08:33:31.02 delay 3 seconds @08:33:34.05 AFV.select :carrierSolution @08:33:34.15 delay 2 seconds @08:33:36.18 AS.reconfigure ASconfig @08:33:36.23 AS.seek 1ml @08:34:34.07 delay 1 second @08:34:35.09 AFV.select :holdingCoil @08:34:35.19 delay 1 second @08:34:36.22 AS.reconfigure ASfast @08:34:36.27 AS.seek 0ml @08:35:03.63 delay 3 seconds @08:35:06.66 AFV.select :carrierSolution @08:35:06.76 delay 2 seconds @08:35:08.79 AS.reconfigure ASconfig @08:35:08.85 AS.seek 1ml @08:36:06.69 delay 1 second @08:36:07.71 AFV.select :holdingCoil @08:36:07.81 delay 1 second @08:36:08.84 AS.reconfigure ASfast @08:36:08.89 AS.seek 0ml @08:36:36.24 delay 3 seconds @08:36:39.27 AFV.select :carrierSolution @08:36:39.37 delay 2 seconds @08:36:41.40 AS.reconfigure ASconfig @08:36:41.45 AS.seek 1ml @08:37:39.24 delay 1 second @08:37:40.26 AFV.select :holdingCoil @08:37:40.36 delay 1 second @08:37:41.39 AS.reconfigure ASfast @08:37:41.44 AS.seek 0ml @08:38:08.77 delay 3 seconds @08:38:11.80 AFV.select :carrierSolution @08:38:11.90 delay 2 seconds @08:38:13.93 AS.reconfigure ASconfig @08:38:13.99 AS.seek 1ml @08:39:11.76 delay 1 second @08:39:12.78 AFV.select :holdingCoil @08:39:12.88 delay 1 second @08:39:13.91 AS.reconfigure ASnorm @08:39:13.96 AS.seek 0ml @08:40:04.66 AFV.select :carrierSolution @08:40:04.77 delay 2 seconds @08:40:06.80 AS.reconfigure ASconfig @08:40:06.85 AS.seek 1ml @08:41:04.65 delay 1 second @08:41:05.67 AFV.select :holdingCoil @08:41:05.77 delay 1 second @08:41:06.80 ATV.dial :waste @08:41:07.94 AS.reconfigure ASnorm @08:41:07.99 AS.seek 0.5ml @08:41:33.54 ATV.dial :waste2 @08:41:34.75 AS.seek 0ml @08:42:00.27 AS.coast @08:42:00.32 SS.coast @08:42:00.36 CC.coast @08:42:00.39 CS.coast @08:42:00.43 PC.coast @08:42:00.48 PS.coast @08:42:00.52 Forearm.coast @08:42:00.56 Elbow.coast @08:42:00.95 Gate.power :core,:OFF @08:42:00.96 Gate.power :raw,:OFF @08:42:00.97 Shutdown @08:42:00.99 GoodNews.email "MFBPCR completed",Subject: "Shutdown" @08:42:01.02 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @08:42:01.03 Retry #1 of 10 after 10:00 @08:52:01.07 GoodNews.email "MFBPCR completed",Subject: "Shutdown" @08:52:01.10 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @08:52:01.11 Retry #2 of 10 after 10:00 @09:02:01.14 GoodNews.email "MFBPCR completed",Subject: "Shutdown" @09:02:01.17 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @09:02:01.18 Retry #3 of 10 after 10:00 @09:12:01.21 GoodNews.email "MFBPCR completed",Subject: "Shutdown" @09:12:01.24 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @09:12:01.25 Retry #4 of 10 after 10:00 @09:22:01.28 GoodNews.email "MFBPCR completed",Subject: "Shutdown" @09:22:01.31 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @09:22:01.32 Retry #5 of 10 after 10:00 @09:32:01.35 GoodNews.email "MFBPCR completed",Subject: "Shutdown" @09:32:01.38 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @09:32:01.39 Retry #6 of 10 after 10:00 @09:42:01.42 GoodNews.email "MFBPCR completed",Subject: "Shutdown" @09:42:01.45 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @09:42:01.46 Retry #7 of 10 after 10:00 @09:52:01.49 GoodNews.email "MFBPCR completed",Subject: "Shutdown" @09:52:01.52 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @09:52:01.53 Retry #8 of 10 after 10:00 @10:02:01.56 GoodNews.email "MFBPCR completed",Subject: "Shutdown" @10:02:01.59 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @10:02:01.60 Retry #9 of 10 after 10:00 @10:12:01.63 GoodNews.email "MFBPCR completed",Subject: "Shutdown" @10:12:01.66 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @10:12:01.67 Retry #10 of 10 after 10:00 @10:22:01.70 GoodNews.email "MFBPCR completed",Subject: "Shutdown" @10:22:01.73 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @20:45:00.05
Powering core bus @20:45:05.18 Gate.power :raw,:ON @20:45:06.38 SPE.coast @20:45:06.44 SPE.configure SPEconfig @20:45:06.54 AS.configure ASconfig @20:45:06.65 SS.configure SSconfig @20:45:06.75 CC.configure CCconfig @20:45:06.85 CS.configure CSconfig @20:45:06.95 PC.configure PCconfig @20:45:07.04 PS.configure PSconfig @20:45:07.17 Forearm.configure LooseForearm @20:45:07.26 Elbow.configure LooseElbow @20:45:07.38 SC.configure SCloose @20:45:07.48 SE.configure SEconfig @20:45:07.53 CH.coast @20:45:07.58 CH.configure CHconfig @20:45:07.62 PH.coast @20:45:07.68 PH.configure PHconfig @20:45:07.72 PCR.off @20:45:07.76 PCR.pidtemp= 50,0.1,0,300,25,25 @20:45:07.79 PCR.caltemp= 2.8814,0.0372,0 @20:45:07.81 All dwarf controllers running firmware version 3.75 @20:45:07.93 Valve.reset @20:45:08.10 PCR.off @20:45:08.13 PCR.pidtemp= 50,0.1,0,300,25,25 @20:45:08.17 PCR.caltemp= 2.8814,0.0372,0 @20:45:08.36 PSV.dial :air @20:45:08.50
Forearm.reconfigure LooseForearm @20:45:08.55 Forearm.home.seek :retract @20:45:08.83 PS.reconfigure PSconfig @20:45:08.89 PS.home.seek 0ml @20:45:08.91 Intake.select :close @20:45:08.95 Exhaust.select :close @20:45:08.98 CTV.dial :air @20:45:09.06 CSV.dial :waste1 @20:45:09.27 Hand.close @20:45:09.30
Elbow.jog 1500 @20:45:09.51 SSV.dial :spit,avoiding: :exhaust @20:45:09.97
Elbow.reconfigure LooseElbow @20:45:10.02 Elbow.home.seek :clear @20:45:10.33 CS.reconfigure CSfast @20:45:10.37 SS.reconfigure SSconfig @20:45:10.46 CS.home.seek 0ml @20:45:10.50 SS.home.seek 0ml @20:45:13.38
SE.reconfigure SEconfig @20:45:13.43 SE.home.seek :down @20:45:14.09 SC.reconfigure SCsloppy @20:45:14.14 SC.home.seek :initial @20:45:16.37 Forearm.seek :retract @20:45:16.45 Elbow.seek :hammer @20:45:17.76 Forearm.reconfigure TightForearm @20:45:17.81 Forearm.seek 5 + 350 counts @20:45:17.95 PS.coast @20:45:18.84
Forearm.seek :retract @20:45:20.05 PC.reconfigure PCconfig @20:45:20.06
CC.reconfigure CCconfig @20:45:20.15 PC.home.seek :open @20:45:20.19
CC.home.seek :open @20:45:23.71 Phase [mbaPhase] @20:45:23.73 GoodNews.email "At 20:45:23 began in tube 2: mbaPhase\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:125\n{1 => 9, 2 => 13}\nCan@20:45:23, 16.4C, 79% humidity, 14.6psia, 14.611V, 0.682A, 0.676A avg, 9.97W",Subject: "Started TotaldeploymentFINAL3 phase mbaPhase" @20:45:23.75CEST11-Sep-19
==================> STARTED Superphase (phase #2) <=== @20:45:23.79 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @20:45:23.81 Starting initialPurge @20:45:23.83 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @20:45:23.85 Retry #1 of 10 after 10:00 @20:45:23.94
Hand.open @20:45:24.70 Elbow.reconfigure TightElbow @20:45:24.75 Elbow.seek FlushPuck::Garage @20:45:26.51 Elbow.reconfigure LooseElbow @20:45:26.62 Forearm.reconfigure LooseForearm @20:45:26.67 Forearm.seek :garage @20:45:27.65 Hand.close @20:45:28.46 Forearm.seek :retract @20:45:29.46 Elbow.reconfigure TightElbow @20:45:29.51 Elbow.seek CC @20:45:32.33 Elbow.reconfigure LooseElbow @20:45:32.44 Forearm.reconfigure TightForearm @20:45:32.49 Forearm.seek CC @20:45:33.82 CC.seek :closed @20:45:38.07 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.65 @20:45:38.16 Hand.open @20:45:38.90 Forearm.reconfigure LooseForearm @20:45:38.95 Forearm.seek :retract @20:45:40.02 Flushing the Collection Valve::Manifold @20:45:40.16 CSV.dial CSR @20:45:41.47 Pulling 5ml of CSR.flush @20:45:41.48 CSR.select :flush @20:45:41.61 CS.reconfigure CSconfig @20:45:41.66 CS.seek 5ml @20:45:55.40 delay 5 seconds @20:46:00.43 CSR.select :air @20:46:00.59 CS.reconfigure CSfast @20:46:00.64 CS.seek 7.5ml @20:46:07.12 CSV.dial :waste @20:46:08.35 CS.reconfigure CSconfig @20:46:08.40 CS.seek 0ml @20:46:28.59 Purging external Intake @20:46:28.72 CSV.dial :flush @20:46:29.17 CS.reconfigure CSfast @20:46:29.22 CS.seek 10ml @20:46:51.59 delay 5 seconds @20:46:56.62 CSV.dial CBV @20:46:56.65
CBV.dial CSV @20:46:57.40 CTV.dial :intake @20:46:58.15 Intake.select :open @20:46:58.27 CS.seek 0ml @20:47:21.99 Intake.select :close @20:47:22.10 CTV.dial :air @20:47:22.84 Flushing upper manifold @20:47:22.97 CSV.dial :flush @20:47:23.71 CS.seek 5ml @20:47:35.49 delay 5 seconds @20:47:40.52 CSV.dial :air @20:47:41.05 CS.seek 7ml @20:47:46.40 CSV.dial CBV @20:47:46.44
CBV.dial CSV @20:47:47.45 CTV.dial :air @20:47:47.47 CS.seek 0ml @20:48:03.34 delay 7 seconds @20:48:10.38 CS.seek 3ml @20:48:17.81 CSV.dial :waste @20:48:18.29 CS.seek 0ml @20:48:25.90 CSV.dial :flush @20:48:26.36 CS.seek 3ml @20:48:33.87 delay 5 seconds @20:48:38.90 CSV.dial :air @20:48:39.96 CS.seek 5ml @20:48:45.43 CSV.dial :air @20:48:45.45 CS.seek 0ml @20:48:57.00 delay 7 seconds @20:49:04.04 CS.seek 3ml @20:49:11.53 CSV.dial :waste @20:49:12.27 CS.seek 0ml @20:49:19.62 Garaging FlushPuck in Collection Clamp @20:49:19.70 Elbow.reconfigure TightElbow @20:49:19.75 Elbow.seek CC @20:49:20.02 Elbow.reconfigure LooseElbow @20:49:20.12 Forearm.seek CC @20:49:21.14 Hand.close @20:49:21.70 CC.seek :open @20:49:26.03 Forearm.seek :retract @20:49:27.08 Elbow.reconfigure TightElbow @20:49:27.13 Elbow.seek :garage @20:49:30.12 Elbow.reconfigure LooseElbow @20:49:30.23 Forearm.reconfigure TightForearm @20:49:30.28 Forearm.seek :garage @20:49:31.46 Hand.open @20:49:32.15 Forearm.reconfigure LooseForearm @20:49:32.20 Forearm.seek :retract @20:49:33.29 Starting mfb @20:49:33.30 pcrBAC sampling at most 2000ml, wcr at most 2000ml @20:49:33.39 Loading sh1 puck from tube 2 into Collection Clamp @20:49:33.54 Readying the MFB @20:49:33.55 Homing MFB Syringe @20:49:33.56 AFV.select :holdingCoil @20:49:33.80 ATV.dial :air2 @20:49:33.93
SC.reconfigure SCloose @20:49:34.16 Elbow.reconfigure TightElbow @20:49:34.21 Elbow.seek 2 @20:49:34.41 AS.reconfigure ASconfig @20:49:34.46 AS.home.seek :home @20:49:36.26
Forearm.reconfigure TightForearm @20:49:36.31 Forearm.seek 2 + 700 counts @20:49:37.35 SE.reconfigure SEpick @20:49:37.43 SE.seek beamBreak @20:49:39.38 SE.configure SEconfig @20:49:39.55 Elbow.seek 2 @20:49:39.84 Forearm.seek 2 + 300 counts @20:49:39.99 AFV.select :carrierSolution @20:49:40.06
Forearm.reconfigure LooseForearm @20:49:40.11 Hand.close @20:49:40.16 delay 2 seconds @20:49:40.72 SE.seek :down @20:49:40.95
Forearm.seek :retract @20:49:41.90 Elbow.seek CC @20:49:42.19 AS.seek 1ml @20:49:42.52 SE.coast @20:49:43.69
Elbow.reconfigure LooseElbow @20:49:43.91 Forearm.reconfigure TightForearm @20:49:44.01 Forearm.seek CC @20:49:45.92 CC.seek :closed @20:49:50.14 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.77 @20:49:50.15 Hand.open @20:49:50.90 Forearm.reconfigure LooseForearm @20:49:50.95 Forearm.seek :retract @20:49:52.03 SC.coast @20:49:52.07 collectSample 2000ml w/bubble point of 55psi @20:49:52.08 Priming 7ml @20:49:52.09 Intake.select :close @20:49:52.10 Exhaust.select :close @20:49:52.12 SS.hold @20:49:52.35 Calibrating pressure and temperature sensors @20:49:52.36 CTV.dial :air @20:49:52.38 SSV.dial :spit,avoiding: :exhaust @20:49:52.40 SS.reconfigure SSfast @20:49:52.45 SS.seek 5ml @20:49:55.14 SSV.dial CBV @20:49:55.16
CBV.dial SSV @20:49:56.48 delay 3.8 seconds @20:50:00.44 SSV.dial :spit @20:50:01.74 SS.seek 0ml @20:50:04.28 SSV.dial CBV @20:50:05.57 CTV.dial :intake @20:50:06.32 SSV.dial CBV @20:50:06.35
CBV.dial SSV @20:50:06.37 Allowing 40s for 7.0ml of remaining 7.0ml @20:50:06.38 Intake.select :open @20:50:08.57 Intake @32.1psia, vacuum from 20.0 to 24.0psi @20:50:08.59 SS.reconfigure SSinhale.with maxSpeed: 150 @20:50:08.64 SS.seek 7ml @20:50:12.30 Vacuum too high -- waiting up to 40s @20:50:14.16 Intake @31.8psia, vacuum from 20.0 to 24.0psi @20:50:14.18 SS.reconfigure SSinhale.with maxSpeed: 135 @20:50:14.23 SS.seek 7ml @20:50:17.20 Stroke completed -- waiting for pressure to equalize ... @20:50:20.46 Intake.select :close @20:50:20.62 SSV.dial :exhaust @20:50:21.36 Exhaust.select :open @20:50:21.48 SS.reconfigure SSexhale @20:50:21.52 SS.seek 0ml @20:50:25.01 Exhaust.select :close @20:50:25.12 Sampling 2000.0ml @20:50:25.13 Intake.select :close @20:50:25.15 Exhaust.select :close @20:50:25.16 SS.reconfigure SSconfig @20:50:25.21 SS.hold @20:50:25.43 CTV.dial :intake @20:50:25.45 SSV.dial CBV @20:50:25.47
CBV.dial SSV @20:50:26.26 Allowing 130s for 25.0ml of remaining 2000.0ml @20:50:26.27 Intake.select :open @20:50:28.44 Intake @32.7psia, vacuum from 20.0 to 24.0psi @20:50:28.46 SS.reconfigure SSinhale.with maxSpeed: 135 @20:50:28.51 SS.seek 25ml @20:50:32.32 Vacuum too high -- waiting up to 40s @20:50:33.95 Intake @31.1psia, vacuum from 20.0 to 24.0psi @20:50:33.97 SS.reconfigure SSinhale.with maxSpeed: 122 @20:50:34.02 SS.seek 25ml @20:50:35.10 delay 1 second @20:50:36.12 AFV.select :holdingCoil @20:50:36.22 delay 1 second @20:50:37.24 ATV.dial :waste @20:50:37.43
Vacuum too high -- waiting up to 40s @20:50:38.24 AS.reconfigure ASnorm @20:50:38.30 AS.home.seek :empty @20:50:39.33
Intake @31.6psia, vacuum from 20.0 to 24.0psi @20:50:39.35 SS.reconfigure SSinhale.with maxSpeed: 110 @20:50:39.40 SS.seek 25ml @20:50:42.85 Vacuum too high -- waiting up to 40s @20:50:44.45 Intake @32.3psia, vacuum from 20.0 to 24.0psi @20:50:44.47 SS.reconfigure SSinhale.with maxSpeed: 99 @20:50:44.52 SS.seek 25ml @20:50:47.89 Vacuum too high -- waiting up to 40s @20:50:49.07 Intake @30.4psia, vacuum from 20.0 to 24.0psi @20:50:49.09 SS.reconfigure SSinhale.with maxSpeed: 89 @20:50:49.13 SS.seek 25ml @20:50:54.79 Vacuum too high -- waiting up to 40s @20:50:56.67 Intake @30.6psia, vacuum from 20.0 to 24.0psi @20:50:56.68 SS.reconfigure SSinhale.with maxSpeed: 80 @20:50:56.73 SS.seek 25ml @20:51:00.95 Stroke completed -- waiting for pressure to equalize ... @20:51:04.20 Intake.select :close @20:51:04.35 SSV.dial :exhaust @20:51:05.11 Exhaust.select :open @20:51:05.23 SS.reconfigure SSexhale @20:51:05.27 SS.seek 0ml @20:51:14.21 Exhaust.select :close @20:51:14.33 SSV.dial CBV @20:51:14.35
CBV.dial SSV @20:51:15.14 Allowing 130s for 25.0ml of remaining 1975.0ml @20:51:15.15 Intake.select :open @20:51:17.33 Intake @32.3psia, vacuum from 20.0 to 24.0psi @20:51:17.34 SS.reconfigure SSinhale.with maxSpeed: 80 @20:51:17.39 SS.seek 25ml @20:51:22.04 Vacuum too high -- waiting up to 40s @20:51:23.93 Intake @32.1psia, vacuum from 20.0 to 24.0psi @20:51:23.95 SS.reconfigure SSinhale.with maxSpeed: 72 @20:51:23.99 SS.seek 25ml @20:51:29.02 Vacuum too high -- waiting up to 40s @20:51:30.39 Intake @31.8psia, vacuum from 20.0 to 24.0psi @20:51:30.40 SS.reconfigure SSinhale.with maxSpeed: 65 @20:51:30.45 SS.seek 25ml @20:51:30.85 ATV.dial :waste @20:51:30.86 ARV.dial between carrier and air1 @20:51:30.95 ASV.dial :waste1 @20:51:35.91
Vacuum too high -- waiting up to 40s @20:51:37.25 Intake @31.7psia, vacuum from 20.0 to 24.0psi @20:51:37.27 SS.reconfigure SSinhale.with maxSpeed: 59 @20:51:37.32 SS.seek 25ml @20:51:43.63 Vacuum too high -- waiting up to 40s @20:51:45.22 Intake @31.9psia, vacuum from 20.0 to 24.0psi @20:51:45.24 SS.reconfigure SSinhale.with maxSpeed: 53 @20:51:45.28 SS.seek 25ml @20:51:51.80 Vacuum too high -- waiting up to 40s @20:51:53.37 Intake @32.0psia, vacuum from 20.0 to 24.0psi @20:51:53.38 SS.reconfigure SSinhale.with maxSpeed: 48 @20:51:53.43 SS.seek 25ml @20:51:56.86 Stroke completed -- waiting for pressure to equalize ... @20:52:00.09 Intake.select :close @20:52:00.25 SSV.dial :exhaust @20:52:01.01 Exhaust.select :open @20:52:01.13 SS.reconfigure SSexhale @20:52:01.17 SS.seek 0ml @20:52:09.98 Exhaust.select :close @20:52:10.10 SSV.dial CBV @20:52:10.12
CBV.dial SSV @20:52:10.90 Allowing 130s for 25.0ml of remaining 1950.0ml @20:52:10.91 Intake.select :open @20:52:13.09 Intake @32.2psia, vacuum from 20.0 to 24.0psi @20:52:13.10 SS.reconfigure SSinhale.with maxSpeed: 48 @20:52:13.15 SS.seek 25ml @20:52:21.35 Vacuum too high -- waiting up to 40s @20:52:22.68 Intake @32.3psia, vacuum from 20.0 to 24.0psi @20:52:22.70 SS.reconfigure SSinhale.with maxSpeed: 43 @20:52:22.75 SS.seek 25ml @20:52:32.20 Vacuum too high -- waiting up to 40s @20:52:33.56 Intake @31.9psia, vacuum from 20.0 to 24.0psi @20:52:33.58 SS.reconfigure SSinhale.with maxSpeed: 39 @20:52:33.63 SS.seek 25ml @20:52:50.36 Vacuum too high -- waiting up to 40s @20:52:51.97 Intake @32.1psia, vacuum from 20.0 to 24.0psi @20:52:51.98 SS.reconfigure SSinhale.with maxSpeed: 35 @20:52:52.04 SS.seek 25ml @20:53:02.43 Stroke completed -- waiting for pressure to equalize ... @20:53:06.28 Intake.select :close @20:53:06.44 SSV.dial :exhaust @20:53:07.20 Exhaust.select :open @20:53:07.32 SS.reconfigure SSexhale @20:53:07.36 SS.seek 0ml @20:53:16.15 Exhaust.select :close @20:53:16.27 SSV.dial CBV @20:53:16.29
CBV.dial SSV @20:53:17.07 Allowing 130s for 25.0ml of remaining 1925.0ml @20:53:17.08 Intake.select :open @20:53:19.26 Intake @32.7psia, vacuum from 20.0 to 24.0psi @20:53:19.27 SS.reconfigure SSinhale.with maxSpeed: 35 @20:53:19.33 SS.seek 25ml @20:54:09.66 Stroke completed -- waiting for pressure to equalize ... @20:54:14.13 Intake.select :close @20:54:14.29 SSV.dial :exhaust @20:54:15.05 Exhaust.select :open @20:54:15.16 SS.reconfigure SSexhale @20:54:15.21 SS.seek 0ml @20:54:24.12 Exhaust.select :close @20:54:24.24 SSV.dial CBV @20:54:24.26
CBV.dial SSV @20:54:25.06 Allowing 130s for 25.0ml of remaining 1900.0ml @20:54:25.07 Intake.select :open @20:54:27.24 Intake @31.9psia, vacuum from 20.0 to 24.0psi @20:54:27.26 SS.reconfigure SSinhale.with maxSpeed: 35 @20:54:27.31 SS.seek 25ml @20:55:17.91 Stroke completed -- waiting for pressure to equalize ... @20:55:21.78 Intake.select :close @20:55:21.94 SSV.dial :exhaust @20:55:22.70 Exhaust.select :open @20:55:22.81 SS.reconfigure SSexhale @20:55:22.86 SS.seek 0ml @20:55:23.88 GoodNews.email "At 20:45:23 began in tube 2: mbaPhase\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:125\n{1 => 9, 2 => 13}\nCan@20:45:23, 16.4C, 79% humidity, 14.6psia, 14.611V, 0.682A, 0.676A avg, 9.97W",Subject: "Started TotaldeploymentFINAL3 phase mbaPhase" @20:55:23.91 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @20:55:23.92 Retry #2 of 10 after 10:00 @20:55:31.71
Exhaust.select :close @20:55:31.82 SSV.dial CBV @20:55:31.85
CBV.dial SSV @20:55:32.64 Allowing 130s for 25.0ml of remaining 1875.0ml @20:55:32.65 Intake.select :open @20:55:34.83 Intake @32.4psia, vacuum from 20.0 to 24.0psi @20:55:34.84 SS.reconfigure SSinhale.with maxSpeed: 35 @20:55:34.89 SS.seek 25ml @20:56:25.22 Stroke completed -- waiting for pressure to equalize ... @20:56:28.99 Intake.select :close @20:56:29.15 SSV.dial :exhaust @20:56:29.92 Exhaust.select :open @20:56:30.04 SS.reconfigure SSexhale @20:56:30.08 SS.seek 0ml @20:56:39.01 Exhaust.select :close @20:56:39.13 SSV.dial CBV @20:56:39.15
CBV.dial SSV @20:56:39.93 Allowing 130s for 25.0ml of remaining 1850.0ml @20:56:39.94 Intake.select :open @20:56:42.12 Intake @32.7psia, vacuum from 20.0 to 24.0psi @20:56:42.13 SS.reconfigure SSinhale.with maxSpeed: 35 @20:56:42.18 SS.seek 25ml @20:57:32.56 Stroke completed -- waiting for pressure to equalize ... @20:57:35.78 Intake.select :close @20:57:35.94 SSV.dial :exhaust @20:57:36.70 Exhaust.select :open @20:57:36.83 SS.reconfigure SSexhale @20:57:36.88 SS.seek 0ml @20:57:45.51 Exhaust.select :close @20:57:45.63 SSV.dial CBV @20:57:45.65
CBV.dial SSV @20:57:46.43 Allowing 130s for 25.0ml of remaining 1825.0ml @20:57:46.44 Intake.select :open @20:57:48.62 Intake @32.3psia, vacuum from 20.0 to 24.0psi @20:57:48.63 SS.reconfigure SSinhale.with maxSpeed: 35 @20:57:48.69 SS.seek 25ml @20:58:39.23 Stroke completed -- waiting for pressure to equalize ... @20:58:42.46 Intake.select :close @20:58:42.62 SSV.dial :exhaust @20:58:43.39 Exhaust.select :open @20:58:43.50 SS.reconfigure SSexhale @20:58:43.55 SS.seek 0ml @20:58:52.43 Exhaust.select :close @20:58:52.55 SSV.dial CBV @20:58:52.60
CBV.dial SSV @20:58:53.35 Allowing 130s for 25.0ml of remaining 1800.0ml @20:58:53.36 Intake.select :open @20:58:55.54 Intake @33.1psia, vacuum from 20.0 to 24.0psi @20:58:55.55 SS.reconfigure SSinhale.with maxSpeed: 35 @20:58:55.60 SS.seek 25ml @20:59:45.87 Stroke completed -- waiting for pressure to equalize ... @20:59:49.07 Intake.select :close @20:59:49.23 SSV.dial :exhaust @20:59:50.01 Exhaust.select :open @20:59:50.12 SS.reconfigure SSexhale @20:59:50.17 SS.seek 0ml @20:59:59.12 Exhaust.select :close @20:59:59.23 SSV.dial CBV @20:59:59.26
CBV.dial SSV @21:00:00.03 Allowing 130s for 25.0ml of remaining 1775.0ml @21:00:00.04 Intake.select :open @21:00:02.22 Intake @31.8psia, vacuum from 20.0 to 24.0psi @21:00:02.24 SS.reconfigure SSinhale.with maxSpeed: 35 @21:00:02.28 SS.seek 25ml @21:00:52.39 Stroke completed -- waiting for pressure to equalize ... @21:00:55.50 Intake.select :close @21:00:55.66 SSV.dial :exhaust @21:00:56.42 Exhaust.select :open @21:00:56.54 SS.reconfigure SSexhale @21:00:56.58 SS.seek 0ml @21:01:05.43 Exhaust.select :close @21:01:05.55 SSV.dial CBV @21:01:05.57
CBV.dial SSV @21:01:06.35 Allowing 130s for 25.0ml of remaining 1750.0ml @21:01:06.36 Intake.select :open @21:01:08.53 Intake @32.7psia, vacuum from 20.0 to 24.0psi @21:01:08.55 SS.reconfigure SSinhale.with maxSpeed: 35 @21:01:08.60 SS.seek 25ml @21:01:58.70 Stroke completed -- waiting for pressure to equalize ... @21:02:01.94 Intake.select :close @21:02:02.10 SSV.dial :exhaust @21:02:02.85 Exhaust.select :open @21:02:02.96 SS.reconfigure SSexhale @21:02:03.01 SS.seek 0ml @21:02:11.92 Exhaust.select :close @21:02:12.04 SSV.dial CBV @21:02:12.06
CBV.dial SSV @21:02:12.85 Allowing 130s for 25.0ml of remaining 1725.0ml @21:02:12.86 Intake.select :open @21:02:15.04 Intake @32.3psia, vacuum from 20.0 to 24.0psi @21:02:15.06 SS.reconfigure SSinhale.with maxSpeed: 35 @21:02:15.10 SS.seek 25ml @21:03:05.19 Stroke completed -- waiting for pressure to equalize ... @21:03:08.42 Intake.select :close @21:03:08.57 SSV.dial :exhaust @21:03:09.34 Exhaust.select :open @21:03:09.45 SS.reconfigure SSexhale @21:03:09.50 SS.seek 0ml @21:03:18.40 Exhaust.select :close @21:03:18.51 SSV.dial CBV @21:03:18.54
CBV.dial SSV @21:03:19.32 Allowing 130s for 25.0ml of remaining 1700.0ml @21:03:19.33 Intake.select :open @21:03:21.51 Intake @32.4psia, vacuum from 20.0 to 24.0psi @21:03:21.52 SS.reconfigure SSinhale.with maxSpeed: 35 @21:03:21.57 SS.seek 25ml @21:04:11.62 Stroke completed -- waiting for pressure to equalize ... @21:04:14.83 Intake.select :close @21:04:14.99 SSV.dial :exhaust @21:04:15.74 Exhaust.select :open @21:04:15.85 SS.reconfigure SSexhale @21:04:15.90 SS.seek 0ml @21:04:24.76 Exhaust.select :close @21:04:24.88 SSV.dial CBV @21:04:24.90
CBV.dial SSV @21:04:25.70 Allowing 130s for 25.0ml of remaining 1675.0ml @21:04:25.71 Intake.select :open @21:04:27.88 Intake @32.5psia, vacuum from 20.0 to 24.0psi @21:04:27.90 SS.reconfigure SSinhale.with maxSpeed: 35 @21:04:27.95 SS.seek 25ml @21:05:18.17 Stroke completed -- waiting for pressure to equalize ... @21:05:21.38 Intake.select :close @21:05:21.54 SSV.dial :exhaust @21:05:22.29 Exhaust.select :open @21:05:22.89 SS.reconfigure SSexhale @21:05:23.05 SS.seek 0ml @21:05:23.95 GoodNews.email "At 20:45:23 began in tube 2: mbaPhase\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:125\n{1 => 9, 2 => 13}\nCan@20:45:23, 16.4C, 79% humidity, 14.6psia, 14.611V, 0.682A, 0.676A avg, 9.97W",Subject: "Started TotaldeploymentFINAL3 phase mbaPhase" @21:05:23.98 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @21:05:23.99 Retry #3 of 10 after 10:00 @21:05:32.17
Exhaust.select :close @21:05:32.29 SSV.dial CBV @21:05:32.31
CBV.dial SSV @21:05:33.10 Allowing 130s for 25.0ml of remaining 1650.0ml @21:05:33.11 Intake.select :open @21:05:35.30 Intake @32.2psia, vacuum from 20.0 to 24.0psi @21:05:35.32 SS.reconfigure SSinhale.with maxSpeed: 35 @21:05:35.36 SS.seek 25ml @21:06:25.26 Stroke completed -- waiting for pressure to equalize ... @21:06:28.49 Intake.select :close @21:06:28.65 SSV.dial :exhaust @21:06:29.42 Exhaust.select :open @21:06:29.54 SS.reconfigure SSexhale @21:06:29.58 SS.seek 0ml @21:06:38.45 Exhaust.select :close @21:06:38.57 SSV.dial CBV @21:06:38.59
CBV.dial SSV @21:06:39.40 Allowing 130s for 25.0ml of remaining 1625.0ml @21:06:39.41 Intake.select :open @21:06:41.59 Intake @32.3psia, vacuum from 20.0 to 24.0psi @21:06:41.60 SS.reconfigure SSinhale.with maxSpeed: 35 @21:06:41.65 SS.seek 25ml @21:07:31.95 Stroke completed -- waiting for pressure to equalize ... @21:07:35.17 Intake.select :close @21:07:35.33 SSV.dial :exhaust @21:07:36.10 Exhaust.select :open @21:07:36.22 SS.reconfigure SSexhale @21:07:36.26 SS.seek 0ml @21:07:45.31 Exhaust.select :close @21:07:45.43 SSV.dial CBV @21:07:45.45
CBV.dial SSV @21:07:46.23 Allowing 130s for 25.0ml of remaining 1600.0ml @21:07:46.24 Intake.select :open @21:07:48.41 Intake @32.1psia, vacuum from 20.0 to 24.0psi @21:07:48.43 SS.reconfigure SSinhale.with maxSpeed: 35 @21:07:48.48 SS.seek 25ml @21:08:38.73 Stroke completed -- waiting for pressure to equalize ... @21:08:41.94 Intake.select :close @21:08:42.09 SSV.dial :exhaust @21:08:42.85 Exhaust.select :open @21:08:42.96 SS.reconfigure SSexhale @21:08:43.01 SS.seek 0ml @21:08:51.70 Exhaust.select :close @21:08:51.82 SSV.dial CBV @21:08:51.84
CBV.dial SSV @21:08:52.65 Allowing 130s for 25.0ml of remaining 1575.0ml @21:08:52.66 Intake.select :open @21:08:54.84 Intake @32.1psia, vacuum from 20.0 to 24.0psi @21:08:54.85 SS.reconfigure SSinhale.with maxSpeed: 35 @21:08:54.90 SS.seek 25ml @21:09:45.95 Stroke completed -- waiting for pressure to equalize ... @21:09:49.16 Intake.select :close @21:09:49.32 SSV.dial :exhaust @21:09:50.09 Exhaust.select :open @21:09:50.20 SS.reconfigure SSexhale @21:09:50.25 SS.seek 0ml @21:09:59.21 Exhaust.select :close @21:09:59.33 SSV.dial CBV @21:09:59.84
CBV.dial SSV @21:10:00.76 Allowing 130s for 25.0ml of remaining 1550.0ml @21:10:00.77 Intake.select :open @21:10:02.95 Intake @32.7psia, vacuum from 20.0 to 24.0psi @21:10:02.97 SS.reconfigure SSinhale.with maxSpeed: 35 @21:10:03.02 SS.seek 25ml @21:10:53.51 Stroke completed -- waiting for pressure to equalize ... @21:10:56.75 Intake.select :close @21:10:56.91 SSV.dial :exhaust @21:10:57.66 Exhaust.select :open @21:10:57.78 SS.reconfigure SSexhale @21:10:57.82 SS.seek 0ml @21:11:06.46 Exhaust.select :close @21:11:06.58 SSV.dial CBV @21:11:06.60
CBV.dial SSV @21:11:07.40 Allowing 130s for 25.0ml of remaining 1525.0ml @21:11:07.41 Intake.select :open @21:11:09.58 Intake @33.4psia, vacuum from 20.0 to 24.0psi @21:11:09.60 SS.reconfigure SSinhale.with maxSpeed: 35 @21:11:09.65 SS.seek 25ml @21:12:00.29 Stroke completed -- waiting for pressure to equalize ... @21:12:04.15 Intake.select :close @21:12:04.30 SSV.dial :exhaust @21:12:05.07 Exhaust.select :open @21:12:05.19 SS.reconfigure SSexhale @21:12:05.23 SS.seek 0ml @21:12:14.15 Exhaust.select :close @21:12:14.27 SSV.dial CBV @21:12:14.29
CBV.dial SSV @21:12:15.07 Allowing 130s for 25.0ml of remaining 1500.0ml @21:12:15.08 Intake.select :open @21:12:17.26 Intake @32.8psia, vacuum from 20.0 to 24.0psi @21:12:17.27 SS.reconfigure SSinhale.with maxSpeed: 35 @21:12:17.32 SS.seek 25ml @21:13:07.53 Stroke completed -- waiting for pressure to equalize ... @21:13:11.38 Intake.select :close @21:13:11.54 SSV.dial :exhaust @21:13:12.30 Exhaust.select :open @21:13:12.41 SS.reconfigure SSexhale @21:13:12.46 SS.seek 0ml @21:13:21.45 Exhaust.select :close @21:13:21.57 SSV.dial CBV @21:13:21.59
CBV.dial SSV @21:13:22.38 Allowing 130s for 25.0ml of remaining 1475.0ml @21:13:22.39 Intake.select :open @21:13:24.57 Intake @32.5psia, vacuum from 20.0 to 24.0psi @21:13:24.59 SS.reconfigure SSinhale.with maxSpeed: 35 @21:13:24.63 SS.seek 25ml @21:14:15.09 Stroke completed -- waiting for pressure to equalize ... @21:14:18.91 Intake.select :close @21:14:19.07 SSV.dial :exhaust @21:14:19.82 Exhaust.select :open @21:14:19.94 SS.reconfigure SSexhale @21:14:19.98 SS.seek 0ml @21:14:28.91 Exhaust.select :close @21:14:29.02 SSV.dial CBV @21:14:29.49
CBV.dial SSV @21:14:30.35 Allowing 130s for 25.0ml of remaining 1450.0ml @21:14:30.36 Intake.select :open @21:14:32.54 Intake @32.9psia, vacuum from 20.0 to 24.0psi @21:14:32.56 SS.reconfigure SSinhale.with maxSpeed: 35 @21:14:32.60 SS.seek 25ml @21:15:23.09 Stroke completed -- waiting for pressure to equalize ... @21:15:24.02 GoodNews.email "At 20:45:23 began in tube 2: mbaPhase\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:125\n{1 => 9, 2 => 13}\nCan@20:45:23, 16.4C, 79% humidity, 14.6psia, 14.611V, 0.682A, 0.676A avg, 9.97W",Subject: "Started TotaldeploymentFINAL3 phase mbaPhase" @21:15:24.05 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @21:15:24.07 Retry #4 of 10 after 10:00 @21:15:27.60
Intake.select :close @21:15:27.76 SSV.dial :exhaust @21:15:28.52 Exhaust.select :open @21:15:28.64 SS.reconfigure SSexhale @21:15:28.68 SS.seek 0ml @21:15:37.63 Exhaust.select :close @21:15:37.75 SSV.dial CBV @21:15:37.77
CBV.dial SSV @21:15:38.57 Allowing 130s for 25.0ml of remaining 1425.0ml @21:15:38.58 Intake.select :open @21:15:40.76 Intake @32.0psia, vacuum from 20.0 to 24.0psi @21:15:40.78 SS.reconfigure SSinhale.with maxSpeed: 35 @21:15:40.83 SS.seek 25ml @21:16:31.20 Stroke completed -- waiting for pressure to equalize ... @21:16:35.68 Intake.select :close @21:16:35.84 SSV.dial :exhaust @21:16:36.62 Exhaust.select :open @21:16:36.73 SS.reconfigure SSexhale @21:16:36.78 SS.seek 0ml @21:16:45.65 Exhaust.select :close @21:16:45.77 SSV.dial CBV @21:16:45.81
CBV.dial SSV @21:16:46.55 Allowing 130s for 25.0ml of remaining 1400.0ml @21:16:46.56 Intake.select :open @21:16:48.75 Intake @32.3psia, vacuum from 20.0 to 24.0psi @21:16:48.76 SS.reconfigure SSinhale.with maxSpeed: 35 @21:16:48.81 SS.seek 25ml @21:17:39.23 Stroke completed -- waiting for pressure to equalize ... @21:17:43.69 Intake.select :close @21:17:43.85 SSV.dial :exhaust @21:17:44.60 Exhaust.select :open @21:17:44.71 SS.reconfigure SSexhale @21:17:44.76 SS.seek 0ml @21:17:53.94 Exhaust.select :close @21:17:54.06 SSV.dial CBV @21:17:54.08
CBV.dial SSV @21:17:54.87 Allowing 130s for 25.0ml of remaining 1375.0ml @21:17:54.88 Intake.select :open @21:17:57.06 Intake @32.8psia, vacuum from 20.0 to 24.0psi @21:17:57.07 SS.reconfigure SSinhale.with maxSpeed: 35 @21:17:57.12 SS.seek 25ml @21:18:47.04 Stroke completed -- waiting for pressure to equalize ... @21:18:52.10 Intake.select :close @21:18:52.26 SSV.dial :exhaust @21:18:53.02 Exhaust.select :open @21:18:53.14 SS.reconfigure SSexhale @21:18:53.18 SS.seek 0ml @21:19:02.10 Exhaust.select :close @21:19:02.22 SSV.dial CBV @21:19:02.24
CBV.dial SSV @21:19:03.04 Allowing 130s for 25.0ml of remaining 1350.0ml @21:19:03.05 Intake.select :open @21:19:05.23 Intake @33.1psia, vacuum from 20.0 to 24.0psi @21:19:05.24 SS.reconfigure SSinhale.with maxSpeed: 35 @21:19:05.29 SS.seek 25ml @21:19:47.10 Vacuum too high -- waiting up to 40s @21:19:48.18 Intake @32.2psia, vacuum from 20.0 to 24.0psi @21:19:48.20 SS.reconfigure SSinhale.with maxSpeed: 32 @21:19:48.25 SS.seek 25ml @21:19:58.04 Stroke completed -- waiting for pressure to equalize ... @21:20:01.90 Intake.select :close @21:20:02.06 SSV.dial :exhaust @21:20:02.82 Exhaust.select :open @21:20:02.93 SS.reconfigure SSexhale @21:20:02.98 SS.seek 0ml @21:20:11.90 Exhaust.select :close @21:20:12.02 SSV.dial CBV @21:20:12.04
CBV.dial SSV @21:20:12.85 Allowing 130s for 25.0ml of remaining 1325.0ml @21:20:12.86 Intake.select :open @21:20:15.04 Intake @32.8psia, vacuum from 20.0 to 24.0psi @21:20:15.05 SS.reconfigure SSinhale.with maxSpeed: 32 @21:20:15.10 SS.seek 25ml @21:21:10.06 Stroke completed -- waiting for pressure to equalize ... @21:21:13.90 Intake.select :close @21:21:14.06 SSV.dial :exhaust @21:21:14.83 Exhaust.select :open @21:21:14.94 SS.reconfigure SSexhale @21:21:14.99 SS.seek 0ml @21:21:23.65 Exhaust.select :close @21:21:23.76 SSV.dial CBV @21:21:23.79
CBV.dial SSV @21:21:24.55 Allowing 130s for 25.0ml of remaining 1300.0ml @21:21:24.56 Intake.select :open @21:21:26.73 Intake @33.4psia, vacuum from 20.0 to 24.0psi @21:21:26.75 SS.reconfigure SSinhale.with maxSpeed: 32 @21:21:26.80 SS.seek 25ml @21:22:22.07 Stroke completed -- waiting for pressure to equalize ... @21:22:27.18 Intake.select :close @21:22:27.34 SSV.dial :exhaust @21:22:28.10 Exhaust.select :open @21:22:28.22 SS.reconfigure SSexhale @21:22:28.26 SS.seek 0ml @21:22:37.01 Exhaust.select :close @21:22:37.13 SSV.dial CBV @21:22:37.15
CBV.dial SSV @21:22:38.44 Allowing 130s for 25.0ml of remaining 1275.0ml @21:22:38.45 Intake.select :open @21:22:40.63 Intake @32.5psia, vacuum from 20.0 to 24.0psi @21:22:40.65 SS.reconfigure SSinhale.with maxSpeed: 32 @21:22:40.69 SS.seek 25ml @21:23:36.05 Stroke completed -- waiting for pressure to equalize ... @21:23:40.60 Intake.select :close @21:23:40.76 SSV.dial :exhaust @21:23:41.53 Exhaust.select :open @21:23:41.65 SS.reconfigure SSexhale @21:23:41.69 SS.seek 0ml @21:23:50.44 Exhaust.select :close @21:23:50.55 SSV.dial CBV @21:23:50.58
CBV.dial SSV @21:23:51.37 Allowing 130s for 25.0ml of remaining 1250.0ml @21:23:51.38 Intake.select :open @21:23:53.56 Intake @33.1psia, vacuum from 20.0 to 24.0psi @21:23:53.58 SS.reconfigure SSinhale.with maxSpeed: 32 @21:23:53.62 SS.seek 25ml @21:24:48.67 Stroke completed -- waiting for pressure to equalize ... @21:24:53.82 Intake.select :close @21:24:53.98 SSV.dial :exhaust @21:24:54.74 Exhaust.select :open @21:24:54.86 SS.reconfigure SSexhale @21:24:54.90 SS.seek 0ml @21:25:03.85 Exhaust.select :close @21:25:03.97 SSV.dial CBV @21:25:03.99
CBV.dial SSV @21:25:04.76 Allowing 130s for 25.0ml of remaining 1225.0ml @21:25:04.77 Intake.select :open @21:25:06.95 Intake @32.6psia, vacuum from 20.0 to 24.0psi @21:25:06.96 SS.reconfigure SSinhale.with maxSpeed: 32 @21:25:07.01 SS.seek 25ml @21:25:24.09 GoodNews.email "At 20:45:23 began in tube 2: mbaPhase\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:125\n{1 => 9, 2 => 13}\nCan@20:45:23, 16.4C, 79% humidity, 14.6psia, 14.611V, 0.682A, 0.676A avg, 9.97W",Subject: "Started TotaldeploymentFINAL3 phase mbaPhase" @21:25:24.12 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @21:25:24.13 Retry #5 of 10 after 10:00 @21:26:02.01
Stroke completed -- waiting for pressure to equalize ... @21:26:07.16 Intake.select :close @21:26:07.32 SSV.dial :exhaust @21:26:08.08 Exhaust.select :open @21:26:08.19 SS.reconfigure SSexhale @21:26:08.24 SS.seek 0ml @21:26:17.24 Exhaust.select :close @21:26:17.35 SSV.dial CBV @21:26:17.38
CBV.dial SSV @21:26:18.16 Allowing 130s for 25.0ml of remaining 1200.0ml @21:26:18.17 Intake.select :open @21:26:20.34 Intake @32.7psia, vacuum from 20.0 to 24.0psi @21:26:20.36 SS.reconfigure SSinhale.with maxSpeed: 32 @21:26:20.41 SS.seek 25ml @21:27:15.39 Stroke completed -- waiting for pressure to equalize ... @21:27:20.57 Intake.select :close @21:27:20.81 SSV.dial :exhaust @21:27:21.60 Exhaust.select :open @21:27:21.72 SS.reconfigure SSexhale @21:27:21.76 SS.seek 0ml @21:27:30.68 Exhaust.select :close @21:27:30.80 SSV.dial CBV @21:27:30.82
CBV.dial SSV @21:27:31.62 Allowing 130s for 25.0ml of remaining 1175.0ml @21:27:31.63 Intake.select :open @21:27:33.81 Intake @33.0psia, vacuum from 20.0 to 24.0psi @21:27:33.82 SS.reconfigure SSinhale.with maxSpeed: 32 @21:27:33.87 SS.seek 25ml @21:28:16.89 Vacuum too high -- waiting up to 40s @21:28:17.99 Intake @32.3psia, vacuum from 20.0 to 24.0psi @21:28:18.01 SS.reconfigure SSinhale.with maxSpeed: 29 @21:28:18.06 SS.seek 25ml @21:28:31.81 Stroke completed -- waiting for pressure to equalize ... @21:28:36.97 Intake.select :close @21:28:37.12 SSV.dial :exhaust @21:28:37.88 Exhaust.select :open @21:28:38.00 SS.reconfigure SSexhale @21:28:38.04 SS.seek 0ml @21:28:47.01 Exhaust.select :close @21:28:47.12 SSV.dial CBV @21:28:47.15
CBV.dial SSV @21:28:47.94 Allowing 130s for 25.0ml of remaining 1150.0ml @21:28:47.95 Intake.select :open @21:28:50.12 Intake @32.8psia, vacuum from 20.0 to 24.0psi @21:28:50.14 SS.reconfigure SSinhale.with maxSpeed: 29 @21:28:50.19 SS.seek 25ml @21:29:50.91 Stroke completed -- waiting for pressure to equalize ... @21:29:56.01 Intake.select :close @21:29:56.17 SSV.dial :exhaust @21:29:56.93 Exhaust.select :open @21:29:57.04 SS.reconfigure SSexhale @21:29:57.09 SS.seek 0ml @21:30:05.66 Exhaust.select :close @21:30:05.77 SSV.dial CBV @21:30:05.80
CBV.dial SSV @21:30:06.60 Allowing 130s for 25.0ml of remaining 1125.0ml @21:30:06.61 Intake.select :open @21:30:08.79 Intake @32.8psia, vacuum from 20.0 to 24.0psi @21:30:08.80 SS.reconfigure SSinhale.with maxSpeed: 29 @21:30:08.85 SS.seek 25ml @21:31:09.99 Stroke completed -- waiting for pressure to equalize ... @21:31:15.60 Intake.select :close @21:31:15.76 SSV.dial :exhaust @21:31:16.54 Exhaust.select :open @21:31:16.66 SS.reconfigure SSexhale @21:31:16.70 SS.seek 0ml @21:31:25.46 Exhaust.select :close @21:31:25.58 SSV.dial CBV @21:31:25.60
CBV.dial SSV @21:31:26.40 Allowing 130s for 25.0ml of remaining 1100.0ml @21:31:26.41 Intake.select :open @21:31:28.60 Intake @32.8psia, vacuum from 20.0 to 24.0psi @21:31:28.61 SS.reconfigure SSinhale.with maxSpeed: 29 @21:31:28.66 SS.seek 25ml @21:32:29.56 Stroke completed -- waiting for pressure to equalize ... @21:32:35.39 Intake.select :close @21:32:35.55 SSV.dial :exhaust @21:32:36.32 Exhaust.select :open @21:32:36.43 SS.reconfigure SSexhale @21:32:36.48 SS.seek 0ml @21:32:45.25 Exhaust.select :close @21:32:45.36 SSV.dial CBV @21:32:45.39
CBV.dial SSV @21:32:46.18 Allowing 130s for 25.0ml of remaining 1075.0ml @21:32:46.19 Intake.select :open @21:32:48.37 Intake @33.4psia, vacuum from 20.0 to 24.0psi @21:32:48.38 SS.reconfigure SSinhale.with maxSpeed: 29 @21:32:48.43 SS.seek 25ml @21:33:26.12 Vacuum too high -- waiting up to 40s @21:33:27.20 Intake @33.1psia, vacuum from 20.0 to 24.0psi @21:33:27.22 SS.reconfigure SSinhale.with maxSpeed: 26 @21:33:27.27 SS.seek 25ml @21:33:53.58 Stroke completed -- waiting for pressure to equalize ... @21:33:58.81 Intake.select :close @21:33:59.05 SSV.dial :exhaust @21:33:59.85 Exhaust.select :open @21:33:59.96 SS.reconfigure SSexhale @21:34:00.01 SS.seek 0ml @21:34:08.94 Exhaust.select :close @21:34:09.06 SSV.dial CBV @21:34:09.08
CBV.dial SSV @21:34:09.85 Allowing 130s for 25.0ml of remaining 1050.0ml @21:34:09.86 Intake.select :open @21:34:12.03 Intake @32.4psia, vacuum from 20.0 to 24.0psi @21:34:12.05 SS.reconfigure SSinhale.with maxSpeed: 26 @21:34:12.10 SS.seek 25ml @21:35:19.65 Stroke completed -- waiting for pressure to equalize ... @21:35:24.16 GoodNews.email "At 20:45:23 began in tube 2: mbaPhase\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:125\n{1 => 9, 2 => 13}\nCan@20:45:23, 16.4C, 79% humidity, 14.6psia, 14.611V, 0.682A, 0.676A avg, 9.97W",Subject: "Started TotaldeploymentFINAL3 phase mbaPhase" @21:35:24.19 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @21:35:24.20 Retry #6 of 10 after 10:00 @21:35:25.56
Intake.select :close @21:35:25.72 SSV.dial :exhaust @21:35:26.49 Exhaust.select :open @21:35:26.61 SS.reconfigure SSexhale @21:35:26.65 SS.seek 0ml @21:35:35.35 Exhaust.select :close @21:35:35.47 SSV.dial CBV @21:35:35.49
CBV.dial SSV @21:35:36.29 Allowing 130s for 25.0ml of remaining 1025.0ml @21:35:36.30 Intake.select :open @21:35:38.47 Intake @32.9psia, vacuum from 20.0 to 24.0psi @21:35:38.49 SS.reconfigure SSinhale.with maxSpeed: 26 @21:35:38.54 SS.seek 25ml @21:36:46.24 Stroke completed -- waiting for pressure to equalize ... @21:36:51.99 Intake.select :close @21:36:52.15 SSV.dial :exhaust @21:36:52.91 Exhaust.select :open @21:36:53.03 SS.reconfigure SSexhale @21:36:53.07 SS.seek 0ml @21:37:01.99 Exhaust.select :close @21:37:02.10 SSV.dial CBV @21:37:02.13
CBV.dial SSV @21:37:02.94 Allowing 130s for 25.0ml of remaining 1000.0ml @21:37:02.95 Intake.select :open @21:37:05.13 Intake @33.0psia, vacuum from 20.0 to 24.0psi @21:37:05.14 SS.reconfigure SSinhale.with maxSpeed: 26 @21:37:05.20 SS.seek 25ml @21:38:12.46 Stroke completed -- waiting for pressure to equalize ... @21:38:18.21 Intake.select :close @21:38:18.37 SSV.dial :exhaust @21:38:19.14 Exhaust.select :open @21:38:19.25 SS.reconfigure SSexhale @21:38:19.30 SS.seek 0ml @21:38:28.03 Exhaust.select :close @21:38:28.14 SSV.dial CBV @21:38:28.17
CBV.dial SSV @21:38:28.94 Allowing 130s for 25.0ml of remaining 975.0ml @21:38:28.95 Intake.select :open @21:38:31.12 Intake @33.5psia, vacuum from 20.0 to 24.0psi @21:38:31.14 SS.reconfigure SSinhale.with maxSpeed: 26 @21:38:31.19 SS.seek 25ml @21:39:39.04 Stroke completed -- waiting for pressure to equalize ... @21:39:46.00 Intake.select :close @21:39:46.60 SSV.dial :exhaust @21:39:47.44 Exhaust.select :open @21:39:47.56 SS.reconfigure SSexhale @21:39:47.60 SS.seek 0ml @21:39:56.61 Exhaust.select :close @21:39:56.73 SSV.dial CBV @21:39:56.75
CBV.dial SSV @21:39:57.53 Allowing 130s for 25.0ml of remaining 950.0ml @21:39:57.54 Intake.select :open @21:39:59.73 Intake @33.1psia, vacuum from 20.0 to 24.0psi @21:39:59.74 SS.reconfigure SSinhale.with maxSpeed: 26 @21:39:59.79 SS.seek 25ml @21:41:07.03 Stroke completed -- waiting for pressure to equalize ... @21:41:14.06 Intake.select :close @21:41:14.22 SSV.dial :exhaust @21:41:14.97 Exhaust.select :open @21:41:15.09 SS.reconfigure SSexhale @21:41:15.13 SS.seek 0ml @21:41:23.89 Exhaust.select :close @21:41:24.01 SSV.dial CBV @21:41:24.03
CBV.dial SSV @21:41:24.81 Allowing 130s for 25.0ml of remaining 925.0ml @21:41:24.83 Intake.select :open @21:41:27.01 Intake @33.1psia, vacuum from 20.0 to 24.0psi @21:41:27.02 SS.reconfigure SSinhale.with maxSpeed: 26 @21:41:27.07 SS.seek 25ml @21:42:23.60 Vacuum too high -- waiting up to 40s @21:42:24.92 Intake @32.8psia, vacuum from 20.0 to 24.0psi @21:42:24.94 SS.reconfigure SSinhale.with maxSpeed: 23 @21:42:24.99 SS.seek 25ml @21:42:37.91 Stroke completed -- waiting for pressure to equalize ... @21:42:44.26 Intake.select :close @21:42:44.42 SSV.dial :exhaust @21:42:45.18 Exhaust.select :open @21:42:45.30 SS.reconfigure SSexhale @21:42:45.34 SS.seek 0ml @21:42:54.46 Exhaust.select :close @21:42:54.58 SSV.dial CBV @21:42:54.60
CBV.dial SSV @21:42:55.38 Allowing 130s for 25.0ml of remaining 900.0ml @21:42:55.39 Intake.select :open @21:42:57.56 Intake @34.0psia, vacuum from 20.0 to 24.0psi @21:42:57.58 SS.reconfigure SSinhale.with maxSpeed: 23 @21:42:57.63 SS.seek 25ml @21:44:13.60 Stroke completed -- waiting for pressure to equalize ... @21:44:20.50 Intake.select :close @21:44:20.66 SSV.dial :exhaust @21:44:21.42 Exhaust.select :open @21:44:21.54 SS.reconfigure SSexhale @21:44:21.58 SS.seek 0ml @21:44:30.24 Exhaust.select :close @21:44:30.36 SSV.dial CBV @21:44:30.38
CBV.dial SSV @21:44:31.16 Allowing 130s for 25.0ml of remaining 875.0ml @21:44:31.17 Intake.select :open @21:44:33.34 Intake @33.3psia, vacuum from 20.0 to 24.0psi @21:44:33.36 SS.reconfigure SSinhale.with maxSpeed: 23 @21:44:33.41 SS.seek 25ml @21:45:24.68 GoodNews.email "At 20:45:23 began in tube 2: mbaPhase\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:125\n{1 => 9, 2 => 13}\nCan@20:45:23, 16.4C, 79% humidity, 14.6psia, 14.611V, 0.682A, 0.676A avg, 9.97W",Subject: "Started TotaldeploymentFINAL3 phase mbaPhase" @21:45:24.71 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @21:45:24.73 Retry #7 of 10 after 10:00 @21:45:50.03
Stroke completed -- waiting for pressure to equalize ... @21:45:57.03 Intake.select :close @21:45:57.19 SSV.dial :exhaust @21:45:57.94 Exhaust.select :open @21:45:58.05 SS.reconfigure SSexhale @21:45:58.10 SS.seek 0ml @21:46:07.13 Exhaust.select :close @21:46:07.24 SSV.dial CBV @21:46:07.27
CBV.dial SSV @21:46:08.07 Allowing 130s for 25.0ml of remaining 850.0ml @21:46:08.08 Intake.select :open @21:46:10.26 Intake @33.2psia, vacuum from 20.0 to 24.0psi @21:46:10.27 SS.reconfigure SSinhale.with maxSpeed: 23 @21:46:10.32 SS.seek 25ml @21:47:26.40 Stroke completed -- waiting for pressure to equalize ... @21:47:34.00 Intake.select :close @21:47:34.16 SSV.dial :exhaust @21:47:34.92 Exhaust.select :open @21:47:35.03 SS.reconfigure SSexhale @21:47:35.08 SS.seek 0ml @21:47:44.00 Exhaust.select :close @21:47:44.12 SSV.dial CBV @21:47:44.14
CBV.dial SSV @21:47:44.94 Allowing 130s for 25.0ml of remaining 825.0ml @21:47:44.95 Intake.select :open @21:47:47.12 Intake @33.2psia, vacuum from 20.0 to 24.0psi @21:47:47.14 SS.reconfigure SSinhale.with maxSpeed: 23 @21:47:47.19 SS.seek 25ml @21:49:03.48 Stroke completed -- waiting for pressure to equalize ... @21:49:11.03 Intake.select :close @21:49:11.19 SSV.dial :exhaust @21:49:11.97 Exhaust.select :open @21:49:12.09 SS.reconfigure SSexhale @21:49:12.13 SS.seek 0ml @21:49:20.89 Exhaust.select :close @21:49:21.01 SSV.dial CBV @21:49:21.03
CBV.dial SSV @21:49:21.80 Allowing 130s for 25.0ml of remaining 800.0ml @21:49:21.81 Intake.select :open @21:49:23.98 Intake @33.5psia, vacuum from 20.0 to 24.0psi @21:49:24.00 SS.reconfigure SSinhale.with maxSpeed: 23 @21:49:24.05 SS.seek 25ml @21:50:13.53 Vacuum too high -- waiting up to 40s @21:50:14.85 Intake @32.8psia, vacuum from 20.0 to 24.0psi @21:50:14.87 SS.reconfigure SSinhale.with maxSpeed: 21 @21:50:14.92 SS.seek 25ml @21:50:45.09 Stroke completed -- waiting for pressure to equalize ... @21:50:52.72 Intake.select :close @21:50:52.88 SSV.dial :exhaust @21:50:53.64 Exhaust.select :open @21:50:53.76 SS.reconfigure SSexhale @21:50:53.80 SS.seek 0ml @21:51:02.81 Exhaust.select :close @21:51:02.93 SSV.dial CBV @21:51:02.95
CBV.dial SSV @21:51:03.75 Allowing 130s for 25.0ml of remaining 775.0ml @21:51:03.76 Intake.select :open @21:51:05.94 Intake @32.9psia, vacuum from 20.0 to 24.0psi @21:51:05.95 SS.reconfigure SSinhale.with maxSpeed: 21 @21:51:06.00 SS.seek 25ml @21:52:29.44 Stroke completed -- waiting for pressure to equalize ... @21:52:37.70 Intake.select :close @21:52:38.30 SSV.dial :exhaust @21:52:39.17 Exhaust.select :open @21:52:39.29 SS.reconfigure SSexhale @21:52:39.33 SS.seek 0ml @21:52:48.41 Exhaust.select :close @21:52:48.53 SSV.dial CBV @21:52:48.55
CBV.dial SSV @21:52:49.33 Allowing 130s for 25.0ml of remaining 750.0ml @21:52:49.34 Intake.select :open @21:52:51.52 Intake @33.5psia, vacuum from 20.0 to 24.0psi @21:52:51.53 SS.reconfigure SSinhale.with maxSpeed: 21 @21:52:51.58 SS.seek 25ml @21:54:06.47 Vacuum too high -- waiting up to 40s @21:54:07.83 Intake @33.2psia, vacuum from 20.0 to 24.0psi @21:54:07.85 SS.reconfigure SSinhale.with maxSpeed: 19 @21:54:07.90 SS.seek 25ml @21:54:17.55 Stroke completed -- waiting for pressure to equalize ... @21:54:26.44 Intake.select :close @21:54:26.60 SSV.dial :exhaust @21:54:27.36 Exhaust.select :open @21:54:27.48 SS.reconfigure SSexhale @21:54:27.52 SS.seek 0ml @21:54:36.38 Exhaust.select :close @21:54:36.50 SSV.dial CBV @21:54:36.52
CBV.dial SSV @21:54:37.31 Allowing 130s for 25.0ml of remaining 725.0ml @21:54:37.32 Intake.select :open @21:54:39.50 Intake @33.6psia, vacuum from 20.0 to 24.0psi @21:54:39.51 SS.reconfigure SSinhale.with maxSpeed: 19 @21:54:39.56 SS.seek 25ml @21:55:24.75 GoodNews.email "At 20:45:23 began in tube 2: mbaPhase\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:125\n{1 => 9, 2 => 13}\nCan@20:45:23, 16.4C, 79% humidity, 14.6psia, 14.611V, 0.682A, 0.676A avg, 9.97W",Subject: "Started TotaldeploymentFINAL3 phase mbaPhase" @21:55:24.78 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @21:55:24.80 Retry #8 of 10 after 10:00 @21:56:11.65
Stroke completed -- waiting for pressure to equalize ... @21:56:21.22 Intake.select :close @21:56:21.38 SSV.dial :exhaust @21:56:22.15 Exhaust.select :open @21:56:22.27 SS.reconfigure SSexhale @21:56:22.31 SS.seek 0ml @21:56:31.52 Exhaust.select :close @21:56:31.64 SSV.dial CBV @21:56:32.22
CBV.dial SSV @21:56:32.97 Allowing 130s for 25.0ml of remaining 700.0ml @21:56:32.98 Intake.select :open @21:56:35.16 Intake @33.7psia, vacuum from 20.0 to 24.0psi @21:56:35.17 SS.reconfigure SSinhale.with maxSpeed: 19 @21:56:35.22 SS.seek 25ml @21:58:06.91 Stroke completed -- waiting for pressure to equalize ... @21:58:17.11 Intake.select :close @21:58:17.27 SSV.dial :exhaust @21:58:18.03 Exhaust.select :open @21:58:18.15 SS.reconfigure SSexhale @21:58:18.19 SS.seek 0ml @21:58:26.95 Exhaust.select :close @21:58:27.07 SSV.dial CBV @21:58:27.09
CBV.dial SSV @21:58:27.86 Allowing 130s for 25.0ml of remaining 675.0ml @21:58:27.87 Intake.select :open @21:58:30.04 Intake @33.1psia, vacuum from 20.0 to 24.0psi @21:58:30.06 SS.reconfigure SSinhale.with maxSpeed: 19 @21:58:30.11 SS.seek 25ml @22:00:02.58 Stroke completed -- waiting for pressure to equalize ... @22:00:13.36 Intake.select :close @22:00:13.52 SSV.dial :exhaust @22:00:14.28 Exhaust.select :open @22:00:14.40 SS.reconfigure SSexhale @22:00:14.44 SS.seek 0ml @22:00:23.41 Exhaust.select :close @22:00:23.53 SSV.dial CBV @22:00:23.55
CBV.dial SSV @22:00:24.34 Allowing 130s for 25.0ml of remaining 650.0ml @22:00:24.35 Intake.select :open @22:00:26.53 Intake @33.7psia, vacuum from 20.0 to 24.0psi @22:00:26.54 SS.reconfigure SSinhale.with maxSpeed: 19 @22:00:26.59 SS.seek 25ml @22:01:10.96 Vacuum too high -- waiting up to 40s @22:01:12.90 Intake @33.4psia, vacuum from 20.0 to 24.0psi @22:01:12.91 SS.reconfigure SSinhale.with maxSpeed: 17 @22:01:12.97 SS.seek 25ml @22:02:06.77 Stroke completed -- waiting for pressure to equalize ... @22:02:17.60 Intake.select :close @22:02:17.76 SSV.dial :exhaust @22:02:18.52 Exhaust.select :open @22:02:18.64 SS.reconfigure SSexhale @22:02:18.68 SS.seek 0ml @22:02:27.67 Exhaust.select :close @22:02:27.79 SSV.dial CBV @22:02:27.81
CBV.dial SSV @22:02:28.61 Allowing 130s for 25.0ml of remaining 625.0ml @22:02:28.62 Intake.select :open @22:02:30.80 Intake @33.2psia, vacuum from 20.0 to 24.0psi @22:02:30.82 SS.reconfigure SSinhale.with maxSpeed: 17 @22:02:30.87 SS.seek 25ml @22:04:13.63 Stroke completed -- waiting for pressure to equalize ... @22:04:24.99 Intake.select :close @22:04:25.15 SSV.dial :exhaust @22:04:25.92 Exhaust.select :open @22:04:26.03 SS.reconfigure SSexhale @22:04:26.08 SS.seek 0ml @22:04:35.20 Exhaust.select :close @22:04:35.31 SSV.dial CBV @22:04:35.34
CBV.dial SSV @22:04:36.11 Allowing 130s for 25.0ml of remaining 600.0ml @22:04:36.12 Intake.select :open @22:04:38.30 Intake @33.7psia, vacuum from 20.0 to 24.0psi @22:04:38.31 SS.reconfigure SSinhale.with maxSpeed: 17 @22:04:38.36 SS.seek 25ml @22:05:24.82 GoodNews.email "At 20:45:23 began in tube 2: mbaPhase\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:125\n{1 => 9, 2 => 13}\nCan@20:45:23, 16.4C, 79% humidity, 14.6psia, 14.611V, 0.682A, 0.676A avg, 9.97W",Subject: "Started TotaldeploymentFINAL3 phase mbaPhase" @22:05:24.85 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @22:05:24.86 Retry #9 of 10 after 10:00 @22:05:51.56
Vacuum too high -- waiting up to 40s @22:05:52.92 Intake @33.5psia, vacuum from 20.0 to 24.0psi @22:05:52.94 SS.reconfigure SSinhale.with maxSpeed: 15 @22:05:52.99 SS.seek 25ml @22:06:27.00 Stroke completed -- waiting for pressure to equalize ... @22:06:42.22 Intake.select :close @22:06:42.38 SSV.dial :exhaust @22:06:43.16 Exhaust.select :open @22:06:43.28 SS.reconfigure SSexhale @22:06:43.32 SS.seek 0ml @22:06:52.47 Exhaust.select :close @22:06:52.59 SSV.dial CBV @22:06:52.61
CBV.dial SSV @22:06:53.41 Allowing 130s for 25.0ml of remaining 575.0ml @22:06:53.42 Intake.select :open @22:06:55.60 Intake @33.4psia, vacuum from 20.0 to 24.0psi @22:06:55.61 SS.reconfigure SSinhale.with maxSpeed: 15 @22:06:55.66 SS.seek 25ml @22:08:52.33 Stroke completed -- waiting for pressure to equalize ... @22:09:03.43 SS.reconfigure SSconfig @22:09:03.48 SS.hold @22:09:03.61 Intake.select :close @22:09:03.76 ShallowSampler::Clogged in MAIN -- progress too slow after 1450.0ml @22:09:03.77 SSV.dial :exhaust @22:09:04.55 Exhaust.select :open @22:09:04.66 SS.reconfigure SSexhale @22:09:04.71 SS.seek 0ml @22:09:14.02 Exhaust.select :close @22:09:14.15 Sampled 1450.0ml @22:09:14.17 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @22:09:14.18 Intake.select :close @22:09:14.25 CSV.dial :waste @22:09:14.34 CS.seek 0ml @22:09:14.42 SSV.dial CBV,avoiding: :exhaust @22:09:14.47
CBV.dial SSV @22:09:14.53 CSV.dial :air @22:09:15.27 CS.seek 10ml @22:09:16.30
CTV.dial :air @22:09:17.06 delay 15 seconds @22:09:32.09 SSV.dial :spit,avoiding: :exhaust @22:09:33.41 CTV.dial CSV,avoiding: :intake @22:09:33.47
SS.seek 0ml @22:09:33.57 SSV.dial CBV,avoiding: :exhaust @22:09:38.16 CBV.dial between SSV and waste2 @22:09:38.56 CSV.dial between air and CTV @22:09:38.88 CS.seek 8.334ml @22:09:43.65 CTV.dial CSV,avoiding: :air @22:09:43.67
CSV.dial CTV @22:09:44.00 CS.reconfigure CSpressurize @22:09:44.04 CS.seek 0ml @22:10:05.36 BubblePoint: Intake @68.7psia, Outlet @13.1psia @22:10:05.37 CBV.dial SSV @22:10:05.72 SS.reconfigure SSconfig @22:10:05.76 SS.seek 10ml @22:10:11.23 delay 15 seconds @22:10:26.38 Evacuated: Intake @36.3psia, Outlet @-0.9psia @22:10:26.39 SSV.dial :spit,avoiding: :exhaust @22:10:27.71 SS.reconfigure SSexhale @22:10:27.75 SS.seek 0ml @22:10:32.71 SS.reconfigure SSfast @22:10:32.76 SS.seek 5ml @22:10:35.40 SSV.dial CBV,avoiding: :exhaust @22:10:35.43
CBV.dial SSV @22:10:36.73 CTV.dial :air,avoiding: :intake @22:10:38.01 CSV.dial CTV @22:10:38.05
SS.seek 10ml @22:10:39.05 CSV.dial :air @22:10:39.51 CS.reconfigure CSfast @22:10:39.57 CS.seek 0ml @22:10:40.48
SSV.dial :spit,avoiding: :exhaust @22:10:41.06 CS.coast @22:10:41.81
SS.reconfigure SSexhale @22:10:41.85 SS.seek 0ml @22:10:46.99 SS.reconfigure SSfast @22:10:47.04 SS.seek 5ml @22:10:49.80 SSV.dial CBV,avoiding: :exhaust @22:10:49.83
CBV.dial SSV @22:10:52.15 SSV.dial :spit,avoiding: :exhaust @22:10:53.45 SS.reconfigure SSconfig @22:10:53.50 SS.seek 0ml @22:10:56.42 SS.coast @22:10:56.46 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @22:10:56.47 Intake.select :close @22:10:56.55 CSV.dial :waste @22:10:56.68 SSV.dial CBV,avoiding: :exhaust @22:10:56.71
CBV.dial SSV @22:10:57.36 CS.seek 0ml @22:10:57.43 CSV.dial :air @22:10:58.03
CTV.dial :air @22:10:58.05 delay 15 seconds @22:10:58.16 CS.seek 10ml @22:11:13.07
SSV.dial :spit,avoiding: :exhaust @22:11:14.88 CTV.dial CSV,avoiding: :intake @22:11:14.93
SS.reconfigure SSexhale @22:11:15.02 SS.seek 0ml @22:11:15.08 SSV.dial CBV,avoiding: :exhaust @22:11:20.97 CBV.dial between SSV and waste2 @22:11:21.38 CSV.dial between air and CTV @22:11:21.72 CS.seek 7.727ml @22:11:27.72 CTV.dial CSV,avoiding: :air @22:11:27.74
CSV.dial CTV @22:11:28.07 CS.reconfigure CSpressurize @22:11:28.11 CS.seek 0ml @22:11:48.53 CSV.dial :air @22:11:48.99 CS.reconfigure CSfast @22:11:49.04 CS.seek 10ml @22:12:11.47 CSV.dial between air and CTV @22:12:11.80 CS.seek 3.038ml @22:12:27.75 CTV.dial CSV,avoiding: :air @22:12:27.77
CSV.dial CTV @22:12:28.10 CS.reconfigure CSpressurize @22:12:28.14 CS.seek 0ml @22:12:35.21 BubblePoint: Intake @67.9psia, Outlet @12.7psia @22:12:35.22 CBV.dial SSV @22:12:35.56 SS.reconfigure SSconfig @22:12:35.61 SS.seek 10ml @22:12:41.01 delay 15 seconds @22:12:56.15 Evacuated: Intake @40.3psia, Outlet @13.5psia @22:12:56.16 SSV.dial :spit,avoiding: :exhaust @22:12:57.45 SS.reconfigure SSexhale @22:12:57.50 SS.seek 0ml @22:13:02.60 SS.reconfigure SSfast @22:13:02.65 SS.seek 5ml @22:13:05.15 SSV.dial CBV,avoiding: :exhaust @22:13:05.18
CBV.dial SSV @22:13:06.50 CTV.dial :air,avoiding: :intake @22:13:07.79 CSV.dial CTV @22:13:07.84
SS.seek 10ml @22:13:08.84 CSV.dial :air @22:13:09.29 CS.reconfigure CSfast @22:13:09.34 CS.seek 0ml @22:13:10.26
SSV.dial :spit,avoiding: :exhaust @22:13:11.57 SS.reconfigure SSexhale @22:13:11.64 CS.coast @22:13:11.66
SS.seek 0ml @22:13:16.84 SS.reconfigure SSfast @22:13:16.89 SS.seek 5ml @22:13:19.39 SSV.dial CBV,avoiding: :exhaust @22:13:19.42
CBV.dial SSV @22:13:21.73 SSV.dial :spit,avoiding: :exhaust @22:13:23.03 SS.reconfigure SSconfig @22:13:23.09 SS.seek 0ml @22:13:26.21 SS.coast @22:13:26.26 Lysing: Adding 1.5ml lysis @22:13:26.27 CBV.dial between CSV and waste1,avoiding: :DBV @22:13:27.73 CTV.dial CSV @22:13:29.01 CSV.dial CSR @22:13:29.81 Pulling 1ml of CSR.lysis @22:13:29.82 CSR.select :lysis @22:13:30.46 CS.reconfigure CSconfig @22:13:30.50 CS.seek 1ml @22:13:34.03 delay 5 seconds @22:13:39.06 CSR.select :air @22:13:39.23 CS.seek 6ml @22:13:53.06 delay 2 seconds @22:13:55.08 CSV.dial :waste1 @22:13:56.34 CS.reconfigure CSfast @22:13:56.39 CS.seek :empty @22:14:10.12 CSV.dial CSR @22:14:11.45 Pulling 1.5ml of CSR.lysis @22:14:11.46 CSR.select :lysis @22:14:11.59 CS.reconfigure CSconfig @22:14:11.63 CS.seek 1.5ml @22:14:16.43 delay 5 seconds @22:14:21.46 CSR.select :air @22:14:21.64 CS.seek 4.5ml @22:14:30.25 delay 2 seconds @22:14:32.27 CSV.dial CTV @22:14:32.74 CS.seek 1.275ml @22:14:42.04 Heating sh1 puck from tube 2 in Collection Clamp to 85C for 5:00 @22:14:42.06 CH.seek 85C,8:00 @22:15:24.89 GoodNews.email "At 20:45:23 began in tube 2: mbaPhase\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:125\n{1 => 9, 2 => 13}\nCan@20:45:23, 16.4C, 79% humidity, 14.6psia, 14.611V, 0.682A, 0.676A avg, 9.97W",Subject: "Started TotaldeploymentFINAL3 phase mbaPhase" @22:15:24.92 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @22:15:24.94 Retry #10 of 10 after 10:00 @22:17:34.74
CH.hold 5:00,85C @22:22:34.92 Collection Puck Heater at 85C after lysing @22:22:34.94 CS.seek 0ml @22:22:39.22 CTV.dial between spare and air,avoiding: :intake @22:22:40.25 CSV.dial :air @22:22:40.84 CS.seek 5ml @22:22:54.56 CSV.dial CTV @22:22:55.00 delay 2 seconds @22:22:57.03 CSV.dial :air @22:22:57.49 delay 2 seconds @22:22:59.51 CSV.dial CTV @22:22:59.98 delay 2 seconds @22:23:02.00 CSV.dial :air @22:23:02.45 delay 2 seconds @22:23:04.48 CS.seek 0ml @22:23:18.44 Recovering Lysate into Collection syringe @22:23:18.45 CSV.dial CBV @22:23:19.44 CBV.dial CSV @22:23:20.38 CS.seek 5.001ml @22:23:34.35 delay 5 seconds @22:23:39.38 CTV.dial :air @22:23:39.92 CS.seek 7ml @22:23:46.07 delay 5 seconds @22:23:51.10 CSV.dial :air2 @22:23:51.56 CS.seek 10ml @22:24:00.16 CSV.dial between air and CTV,avoiding: CBV @22:24:01.31 Lysing Complete @22:24:01.40 Storing sh1 puck in Collection Clamp to tube 1 @22:24:01.59 SC.reconfigure SCtight @22:24:01.64 SC.seek 1 @22:24:03.80 SC.reconfigure SCloose @22:24:04.03 Elbow.reconfigure TightElbow @22:24:04.08 Elbow.seek 1 @22:24:05.96 Forearm.reconfigure TightForearm @22:24:06.01 Forearm.seek 1 + 700 counts @22:24:07.10 SE.reconfigure SEpick @22:24:07.18 SE.seek beamBreak @22:24:09.34 SE.configure SEconfig @22:24:09.51 Elbow.reconfigure LimpElbow @22:24:09.56 Forearm.reconfigure LooseForearm @22:24:09.60 Forearm.seek 1 @22:24:09.92 Elbow.hold @22:24:09.99 Elbow.reconfigure TightElbow @22:24:10.10 SE.seek 8.08 Pucks below Top Plate @22:24:10.68 Forearm.force -20 @22:24:10.72 Forearm.seek 1 + 346 counts @22:24:11.07 Forearm.reconfigure TightForearm @22:24:11.12 Forearm.seek 1 + 700 counts @22:24:11.41 SE.seek 9.08 Pucks below Top Plate @22:24:12.08 Forearm.reconfigure LooseForearm @22:24:12.13 Forearm.seek :retract @22:24:13.04 Elbow.seek CC @22:24:14.71 Elbow.reconfigure LooseElbow @22:24:14.81 Forearm.seek CC @22:24:15.86 Hand.close @22:24:16.50 CC.seek :open @22:24:20.98 Forearm.seek :retract @22:24:22.01 Elbow.reconfigure TightElbow @22:24:22.06 Elbow.seek 1 @22:24:23.97 Elbow.reconfigure LimpElbow @22:24:24.02 Forearm.seek 1 @22:24:25.06 Elbow.hold @22:24:25.13 Elbow.reconfigure TightElbow @22:24:25.18 Hand.open @22:24:25.95 Forearm.force -20 @22:24:26.44 Forearm.seek 1 + 346 counts @22:24:26.86 Forearm.reconfigure TightForearm @22:24:26.91 Forearm.seek 1 + 700 counts @22:24:27.32 SE.seek :down @22:24:28.48
Forearm.reconfigure LooseForearm @22:24:28.53 Forearm.seek :retract @22:24:29.27 SE.coast @22:24:29.45
SC.coast @22:24:29.49 Tranferring lysate to PRV @22:24:29.50 CSV.dial :air @22:24:29.83 CS.seek 10ml @22:24:29.91 CSV.dial between air and CTV @22:24:30.24 AIV.dial :bypass @22:24:30.33 Beginning samplePassC @22:24:30.35 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @22:24:30.49 Hand.open @22:24:30.84 Elbow.seek FlushPuck::Garage @22:24:32.85 Elbow.reconfigure LooseElbow @22:24:32.95 Forearm.seek :garage @22:24:33.96 Hand.close @22:24:34.70 Forearm.seek :retract @22:24:35.70 Elbow.reconfigure TightElbow @22:24:35.75 Elbow.seek CC @22:24:38.64 Elbow.reconfigure LooseElbow @22:24:38.75 Forearm.reconfigure TightForearm @22:24:38.80 Forearm.seek CC @22:24:40.15 CC.seek :closed @22:24:44.41 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.76 @22:24:44.49 Hand.open @22:24:45.14 Forearm.reconfigure LooseForearm @22:24:45.19 Forearm.seek :retract @22:24:46.22 AWV.select :air @22:24:46.23 AIV.dial :bypass @22:24:46.24 PSV.dial PSR @22:24:47.57 Pulling 1.5ml of PSR.flush @22:24:47.58 PSR.select :flush @22:24:47.71 PS.seek 1.5ml @22:25:03.72 delay 5 seconds @22:25:08.75 PSV.dial :waste1 @22:25:09.21 PS.seek 0ml @22:25:24.96 GoodNews.email "At 20:45:23 began in tube 2: mbaPhase\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:125\n{1 => 9, 2 => 13}\nCan@20:45:23, 16.4C, 79% humidity, 14.6psia, 14.611V, 0.682A, 0.676A avg, 9.97W",Subject: "Started TotaldeploymentFINAL3 phase mbaPhase" @22:25:24.99 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @22:25:25.00 BACfans.email "Started mfb in tube 2 at 20:49:33CEST11-Sep-19\n{1 => 9, 2 => 13}\nCan@20:45:23, 16.4C, 79% humidity, 14.6psia, 14.611V, 0.682A, 0.676A avg, 9.97W",Subject: "pcrBAC sampling at most 2000ml, wcr at most 2000ml" @22:25:25.03 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @22:25:25.05 Retry #1 of 10 after 10:00 @22:25:25.25
PSV.dial PSR @22:25:25.70 PSR.select :air @22:25:25.83 PS.seek 2.5ml @22:25:52.03 PRV.dial CBV @22:25:52.08
CBV.dial PRV @22:25:52.86 PSV.dial :mixloop @22:25:53.86 CTV.dial :air,avoiding: :intake @22:25:53.87 PTV.dial :PRVmixing @22:25:54.51 PS.seek 0ml @22:26:20.66 PSV.dial :air @22:26:21.12 PS.seek 2.5ml @22:26:47.19 PSV.dial :mixloop @22:26:47.65 PS.seek 0ml @22:27:13.78 PSV.dial :air @22:27:14.23 PS.seek 2.5ml @22:27:40.44 PSV.dial :mixloop @22:27:40.90 PS.seek 0ml @22:28:07.16 Garaging FlushPuck in Collection Clamp @22:28:07.24 Elbow.reconfigure TightElbow @22:28:07.29 Elbow.seek CC @22:28:07.54 Elbow.reconfigure LooseElbow @22:28:07.64 Forearm.seek CC @22:28:08.70 Hand.close @22:28:09.33 CC.seek :open @22:28:13.67 Forearm.seek :retract @22:28:14.77 Elbow.reconfigure TightElbow @22:28:14.82 Elbow.seek :garage @22:28:17.83 Elbow.reconfigure LooseElbow @22:28:17.94 Forearm.reconfigure TightForearm @22:28:17.99 Forearm.seek :garage @22:28:19.19 Hand.open @22:28:19.90 Forearm.reconfigure LooseForearm @22:28:19.95 Forearm.seek :retract @22:28:21.02 Connecting PS to CS to transfer sample @22:28:21.03 CBV.dial AIV @22:28:21.04 AIV.dial :bypass @22:28:21.06 PSV.dial :mixloop @22:28:21.07 PTV.dial :PRVmixing @22:28:21.08 PRV.dial AIV @22:28:21.09 CTV.dial :air,avoiding: :intake @22:28:21.11 Loading lyfil puck from tube 2 into Collection Clamp @22:28:21.37 SC.reconfigure SCtight @22:28:21.42 SC.seek 2 @22:28:23.58 SC.reconfigure SCloose @22:28:23.81 Elbow.reconfigure TightElbow @22:28:23.86 Elbow.seek 2 @22:28:25.91 Forearm.reconfigure TightForearm @22:28:25.96 Forearm.seek 2 + 700 counts @22:28:27.06 SE.reconfigure SEpick @22:28:27.14 SE.seek beamBreak @22:28:29.02 SE.configure SEconfig @22:28:29.19 Elbow.seek 2 @22:28:29.38 Forearm.seek 2 + 300 counts @22:28:29.58 Forearm.reconfigure LooseForearm @22:28:29.63 Hand.close @22:28:30.22 SE.seek :down @22:28:30.46
Forearm.seek :retract @22:28:31.43 Elbow.seek CC @22:28:32.05 SE.coast @22:28:33.10
Elbow.reconfigure LooseElbow @22:28:33.20 Forearm.reconfigure TightForearm @22:28:33.25 Forearm.seek CC @22:28:35.17 CC.seek :closed @22:28:39.44 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.80 @22:28:39.45 Hand.open @22:28:40.15 Forearm.reconfigure LooseForearm @22:28:40.20 Forearm.seek :retract @22:28:41.24 SC.coast @22:28:41.28 CTV.dial CSV,avoiding: :intake @22:28:42.57 CSV.dial CTV @22:28:42.89 CS.seek 5ml @22:28:56.73 delay 1:20 @22:30:16.77 CS.seek 2.5ml @22:30:24.16 delay 40 seconds @22:31:04.20 CS.seek 0ml @22:31:11.60 delay 1:00 @22:32:11.63 PSV.dial :air @22:32:12.09 PS.seek 2.5ml @22:32:38.19 PSV.dial :mixloop @22:32:38.63 delay 3 seconds @22:32:41.66 PSV.dial :air @22:32:42.11 delay 2 seconds @22:32:44.13 PSV.dial :mixloop @22:32:44.58 delay 3 seconds @22:32:47.61 PSV.dial :waste1 @22:32:48.86 PS.seek :empty @22:33:15.03 CBV.dial :waste1 @22:33:15.51 delay 5 seconds @22:33:20.54 CBV.dial AIV @22:33:20.99 delay 10 seconds @22:33:31.02 CBV.dial :waste1 @22:33:31.49 delay 10 seconds @22:33:41.52 CBV.dial AIV @22:33:42.01 delay 10 seconds @22:33:52.05 CTV.dial CSV,avoiding: :intake @22:33:52.06 CBV.dial between AIV and waste1 @22:33:52.42 CSV.dial :air @22:33:52.90 CS.seek 10ml @22:34:19.35 CSV.dial CTV @22:34:19.82 CS.seek 0ml @22:34:46.29 CBV.dial :waste1 @22:34:46.60 delay 10 seconds @22:34:56.63 CTV.dial :air,avoiding: :intake @22:34:57.97 Storing lyfil puck in Collection Clamp to tube 1 @22:34:58.16 SC.reconfigure SCtight @22:34:58.21 SC.seek 1 @22:35:00.32 SC.reconfigure SCloose @22:35:00.55 Elbow.reconfigure TightElbow @22:35:00.60 Elbow.seek 1 @22:35:02.46 Forearm.reconfigure TightForearm @22:35:02.51 Forearm.seek 1 + 700 counts @22:35:03.58 SE.reconfigure SEpick @22:35:03.66 SE.seek beamBreak @22:35:05.80 SE.configure SEconfig @22:35:05.96 Elbow.reconfigure LimpElbow @22:35:06.01 Forearm.reconfigure LooseForearm @22:35:06.06 Forearm.seek 1 @22:35:06.37 Elbow.hold @22:35:06.44 Elbow.reconfigure TightElbow @22:35:06.55 SE.seek 9.06 Pucks below Top Plate @22:35:07.03 Forearm.force -20 @22:35:07.07 Forearm.seek 1 + 339 counts @22:35:07.39 Forearm.reconfigure TightForearm @22:35:07.97 Forearm.seek 1 + 700 counts @22:35:08.27 SE.seek 10.06 Pucks below Top Plate @22:35:08.90 Forearm.reconfigure LooseForearm @22:35:08.95 Forearm.seek :retract @22:35:09.90 Elbow.seek CC @22:35:11.56 Elbow.reconfigure LooseElbow @22:35:11.66 Forearm.seek CC @22:35:12.71 Hand.close @22:35:13.34 CC.seek :open @22:35:17.80 Forearm.seek :retract @22:35:18.84 Elbow.reconfigure TightElbow @22:35:18.89 Elbow.seek 1 @22:35:20.83 Elbow.reconfigure LimpElbow @22:35:20.89 Forearm.seek 1 @22:35:21.93 Elbow.hold @22:35:22.00 Elbow.reconfigure TightElbow @22:35:22.05 Hand.open @22:35:22.85 Forearm.force -20 @22:35:22.90 Forearm.seek 1 + 345 counts @22:35:23.21 Forearm.reconfigure TightForearm @22:35:23.26 Forearm.seek 1 + 700 counts @22:35:23.65 SE.seek :down @22:35:24.81
Forearm.reconfigure LooseForearm @22:35:24.86 Forearm.seek :retract @22:35:25.07 BACfans.email "Started mfb in tube 2 at 20:49:33CEST11-Sep-19\n{1 => 9, 2 => 13}\nCan@20:45:23, 16.4C, 79% humidity, 14.6psia, 14.611V, 0.682A, 0.676A avg, 9.97W",Subject: "pcrBAC sampling at most 2000ml, wcr at most 2000ml" @22:35:25.10 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @22:35:25.12 Retry #2 of 10 after 10:00 @22:35:25.49 SE.coast @22:35:25.79
SC.coast @22:35:25.83 PSV.dial :air @22:35:26.89 PS.seek 0.499ml @22:35:32.82 delay 3 seconds @22:35:35.84 PSV.dial :mixloop @22:35:36.28 PRV.dial :air @22:35:36.74 delay 3 seconds @22:35:39.77 PRV.dial CBV @22:35:40.22 CBV.dial AIV @22:35:40.70 PRV.dial between CBV and air @22:35:41.04 PRV.dial :air @22:35:41.43 PS.reconfigure PSslow @22:35:41.48 PS.seek 0.599ml @22:35:44.03 PRV.dial :waste2 @22:35:44.72 PS.reconfigure PSconfig @22:35:44.77 PS.seek :empty @22:35:51.72 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @22:35:51.79 Hand.open @22:35:52.22 Elbow.seek FlushPuck::Garage @22:35:54.24 Elbow.reconfigure LooseElbow @22:35:54.36 Forearm.seek :garage @22:35:55.40 Hand.close @22:35:56.09 Forearm.seek :retract @22:35:57.14 Elbow.reconfigure TightElbow @22:35:57.19 Elbow.seek CC @22:36:00.62 Elbow.reconfigure LooseElbow @22:36:00.73 Forearm.reconfigure TightForearm @22:36:00.78 Forearm.seek CC @22:36:02.16 CC.seek :closed @22:36:06.42 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.73 @22:36:06.50 Hand.open @22:36:07.17 Forearm.reconfigure LooseForearm @22:36:07.22 Forearm.seek :retract @22:36:08.35 Clearing PML for modified lysate @22:36:08.36 CTV.dial :air,avoiding: :intake @22:36:08.37 PRV.dial :waste2 @22:36:08.39 PTV.dial :PRV,avoiding: :puck @22:36:09.10 delay 2 seconds @22:36:11.12 PTV.dial :PRVmixing,avoiding: :puck @22:36:11.83 delay 2 seconds @22:36:13.86 PSV.dial :PML @22:36:13.87 delay 2 seconds @22:36:15.90 PRV.dial :air @22:36:16.63 delay 2 seconds @22:36:18.71 PS.seek 1.499ml @22:36:34.69 delay 5 seconds @22:36:39.73 PS.seek 2.5ml @22:36:50.72 PSV.dial :air @22:36:51.17 PS.seek 0ml @22:37:17.25 mixing lysate with speDil @22:37:17.26 PSV.dial :PML @22:37:17.71 CBV.dial :PRV @22:37:17.72 PRV.dial :air @22:37:17.74 delay 2 seconds @22:37:19.76 PRV.dial :speDil @22:37:20.22 delay 2 seconds @22:37:22.32 PS.reconfigure PSslow1 @22:37:22.37 PS.seek 0.225ml @22:37:46.07 delay 5 seconds @22:37:51.10 PRV.dial CBV @22:37:51.83 delay 2 seconds @22:37:53.91 PS.seek 0.475ml @22:38:19.82 delay 5 seconds @22:38:24.85 PRV.dial :air @22:38:25.32 delay 2 seconds @22:38:27.36 PS.seek 0.965ml @22:39:17.72 delay 10 seconds @22:39:27.75 PRV.dial CBV @22:39:28.21 CTV.dial :air,avoiding: :intake @22:39:28.22 PSV.dial :air @22:39:28.68 PS.reconfigure PSconfig @22:39:28.73 PS.seek 2.5ml @22:39:45.10 delay 3 seconds @22:39:48.12 PSV.dial PTV @22:39:48.85 PTV.dial :PRV,avoiding: :puck @22:39:49.58 PS.seek 0ml @22:40:15.72 PSV.dial :air @22:40:16.45 PS.seek 2.5ml @22:40:42.60 delay 3 seconds @22:40:45.62 PSV.dial PTV @22:40:46.44 PTV.dial :PRV,avoiding: :puck @22:40:46.46 PS.seek 0ml @22:41:12.60 Pushing modified lysate into AIV Injection Loop @22:41:12.61 CBV.dial AIV @22:41:12.62 AIV.dial :bypass @22:41:12.63 PRV.dial CBV @22:41:12.65 PSV.dial :air @22:41:13.37 PS.seek 2.5ml @22:41:39.44 delay 3 seconds @22:41:42.46 PSV.dial :PML @22:41:42.90 PTV.dial :PRVmixing,avoiding: :puck @22:41:44.23 PS.reconfigure PSslow1 @22:41:44.28 PS.seek 1.74ml @22:43:01.85 delay 5 seconds @22:43:06.88 AIV.dial :sample @22:43:07.44 AWV.select :waste @22:43:07.61 PS.reconfigure PSslow2 @22:43:07.66 PS.seek 1.22ml @22:44:56.29 delay 15 seconds @22:45:11.32 AIV.dial :bypass @22:45:11.88 PS.reconfigure PSconfig @22:45:11.93 PS.seek 0ml @22:45:25.10 PSV.dial :air @22:45:25.15 BACfans.email "Started mfb in tube 2 at 20:49:33CEST11-Sep-19\n{1 => 9, 2 => 13}\nCan@20:45:23, 16.4C, 79% humidity, 14.6psia, 14.611V, 0.682A, 0.676A avg, 9.97W",Subject: "pcrBAC sampling at most 2000ml, wcr at most 2000ml" @22:45:25.19 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @22:45:25.20 Retry #3 of 10 after 10:00 @22:45:25.55
PS.seek 2.5ml @22:45:51.70 PSV.dial :PML @22:45:52.16 PS.seek 0ml @22:46:18.35 AIV.dial :coilToMFB @22:46:18.36 AWV.select :air @22:46:18.53 Extracting Nucleic Acid from AIV @22:46:18.56
Beginning samplePassD @22:46:18.60 SPE.hold 2:46:40,55C @22:46:18.69 ASV.dial :SPEdelivery @22:46:18.76
AWV.select :air @22:46:18.78 AIV.dial :bypass @22:46:18.79 PSV.dial PSR @22:46:19.09 delay 20 seconds @22:46:19.88
Pulling 1.5ml of PSR.killMFB @22:46:19.89 PSR.select :killMFB @22:46:20.02 PS.seek 1.5ml @22:46:36.01 delay 5 seconds @22:46:39.12 primeLysateINT @22:46:39.13 AWV.select :air @22:46:39.14 AIV.dial :coilToMFB @22:46:39.16 ATV.dial AIV @22:46:40.77 AS.reconfigure ASslow1 @22:46:40.82 AS.seek 0.299ml @22:46:41.04
PSV.dial :waste1 @22:46:41.52 PS.seek 0ml @22:46:57.57 PSV.dial PSR @22:46:58.04 PSR.select :air @22:46:58.22 PS.seek 2.5ml @22:47:24.48 PRV.dial CBV @22:47:24.51
CBV.dial PRV @22:47:24.52 PSV.dial :mixloop @22:47:25.56 CTV.dial :air,avoiding: :intake @22:47:25.57 PTV.dial :PRVmixing @22:47:25.59 PS.seek 0ml @22:47:41.45 delay 10 seconds @22:47:51.48 ATV.dial :air2 @22:47:51.73
PSV.dial PSR @22:47:52.27 AS.seek 0.329ml @22:47:52.82
Pulling 1.5ml of PSR.flush @22:47:52.83 PSR.select :flush @22:47:52.96 PS.seek 1.5ml @22:47:58.59 ATV.dial :waste2 @22:47:59.12 AS.reconfigure ASnorm @22:47:59.17 AS.seek :empty @22:48:08.98
delay 5 seconds @22:48:14.01 PSV.dial :waste1 @22:48:14.49 PS.seek 0ml @22:48:16.19 Lysate primed! @22:48:16.20 AFV.select :holdingCoil @22:48:16.21 ATV.dial :air2 @22:48:16.82 AS.reconfigure ASslow1 @22:48:16.87 AS.seek 0.069ml @22:48:30.54
PSV.dial PSR @22:48:31.02 PSR.select :air @22:48:31.15 PS.seek 2.5ml @22:48:31.25 delay 3 seconds @22:48:34.27 ATV.dial :AIV @22:48:35.07 AS.seek 0.469ml @22:48:57.26 PRV.dial CBV @22:48:57.29
CBV.dial PRV @22:48:57.30 PSV.dial :mixloop @22:48:58.31 CTV.dial :air,avoiding: :intake @22:48:58.32 PTV.dial :PRVmixing @22:48:58.34 PS.seek 0ml @22:49:25.04 PSV.dial PSR @22:49:26.11 Pulling 1.5ml of PSR.flush @22:49:26.12 PSR.select :flush @22:49:26.25 PS.seek 1.5ml @22:49:42.23 delay 5 seconds @22:49:47.26 PSV.dial :waste1 @22:49:47.72 PS.seek 0ml @22:49:55.81 delay 5 seconds @22:50:00.89 Solid Phase Extraction Heater at 51.5C @22:50:00.90 ATV.dial ASV @22:50:01.28 ASV.dial :SPEdelivery @22:50:01.37 AS.seek 0.041ml @22:50:03.70
PSV.dial PSR @22:50:04.17 PSR.select :air @22:50:04.30 PS.seek 2.5ml @22:50:30.43 PRV.dial CBV @22:50:30.46
CBV.dial PRV @22:50:30.47 PSV.dial :mixloop @22:50:31.46 CTV.dial :air,avoiding: :intake @22:50:31.47 PTV.dial :PRVmixing @22:50:31.49 PS.seek 0ml @22:50:57.60 PSV.dial PSR @22:50:58.65 Pulling 1.5ml of PSR.flush @22:50:58.66 PSR.select :flush @22:50:58.79 PS.seek 1.5ml @22:51:14.79 delay 5 seconds @22:51:19.82 PSV.dial :waste1 @22:51:20.29 PS.seek 0ml @22:51:27.72 delay 10 seconds @22:51:36.29
PSV.dial PSR @22:51:36.74 PSR.select :air @22:51:36.87 PS.seek 2.5ml @22:51:37.74 rinseLysateLineINT @22:51:37.75 AFV.select :carrierSolution @22:51:37.86 delay 2 seconds @22:51:39.89 AS.reconfigure ASconfig @22:51:39.94 AS.seek 1ml @22:52:02.98 PRV.dial CBV @22:52:03.01
CBV.dial PRV @22:52:03.02 PSV.dial :mixloop @22:52:04.03 CTV.dial :air,avoiding: :intake @22:52:04.04 PTV.dial :PRVmixing @22:52:04.06 PS.reconfigure PSslow @22:52:04.11 PS.seek 1.2ml @22:52:30.89 PSV.dial :waste1 @22:52:32.15 PS.reconfigure PSconfig @22:52:32.20 PS.seek 0ml @22:52:35.36 delay 1 second @22:52:36.38 AFV.select :holdingCoil @22:52:36.49 delay 1 second @22:52:37.52 ATV.dial AIV @22:52:38.15 AIV.dial :coilToMFB @22:52:38.16 AWV.select :waste @22:52:38.27 AS.reconfigure ASslow0 @22:52:38.32 AS.seek 0ml @22:52:45.26
PSV.dial :air @22:52:46.26 PS.seek 2.5ml @22:53:12.36 PSV.dial :mixloop @22:53:12.82 PS.reconfigure PSslow @22:53:12.86 PS.seek 0ml @22:54:04.14 PSV.dial :air @22:54:04.59 PS.reconfigure PSconfig @22:54:04.64 PS.seek 2.5ml @22:54:19.22 ATV.dial :air2 @22:54:19.88 AS.reconfigure ASslow1 @22:54:19.93 AS.seek 0.05ml @22:54:30.25 ATV.dial :AIV @22:54:30.79
PSV.dial :mixloop @22:54:31.05 AS.seek 0.01ml @22:54:31.24
PS.reconfigure PSslow @22:54:31.28 PS.seek 0ml @22:54:39.37 ATV.dial :waste @22:54:40.91 AS.seek :empty @22:54:43.33 ethanol wash @22:54:43.34 ATV.dial :air2 @22:54:44.55 AS.reconfigure ASslow2 @22:54:44.60 AS.seek 0.009ml @22:54:54.97 delay 3 seconds @22:54:58.00 Pulling 0.4ml of Ethanol Wash @22:54:58.01 ATV.dial :ethanol @22:54:59.35 AS.reconfigure ASslow1 @22:54:59.40 AS.seek 0.409ml @22:55:22.39
Cleaning Collection Syringe @22:55:22.40 CSV.dial CSR @22:55:22.92 Pulling 4ml of CSR.flush @22:55:22.93 CSR.select :flush @22:55:23.05 CS.seek 4ml @22:55:25.23 BACfans.email "Started mfb in tube 2 at 20:49:33CEST11-Sep-19\n{1 => 9, 2 => 13}\nCan@20:45:23, 16.4C, 79% humidity, 14.6psia, 14.611V, 0.682A, 0.676A avg, 9.97W",Subject: "pcrBAC sampling at most 2000ml, wcr at most 2000ml" @22:55:25.26 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @22:55:25.28 Retry #4 of 10 after 10:00 @22:55:34.21
delay 5 seconds @22:55:39.24 CSR.select :air @22:55:39.37 CS.seek 10ml @22:55:55.78 CSV.dial CTV @22:55:55.81
CTV.dial CSV @22:55:57.20 CBV.dial :waste1 @22:55:57.69 CS.seek 0ml @22:56:20.08 delay 3 seconds @22:56:23.11 ATV.dial :air1 @22:56:23.48 delay 3 seconds @22:56:24.15
CSV.dial CSR @22:56:24.69 Pulling 4ml of CSR.flush @22:56:24.70 CSR.select :flush @22:56:24.82 CS.seek 4ml @22:56:26.58 AS.seek 0.434ml @22:56:31.80 delay 3 seconds @22:56:34.83 ATV.dial :ethanol @22:56:35.43 AS.seek 0.419ml @22:56:36.00
delay 5 seconds @22:56:38.72 delay 3 seconds @22:56:41.03
CSR.select :air @22:56:41.15 CS.seek 10ml @22:56:41.74 ATV.dial ASV @22:56:42.99 AS.seek 0.092ml @22:56:57.46 CSV.dial CTV @22:56:57.49
CTV.dial CSV @22:56:57.96 CBV.dial :waste1 @22:56:57.98 CS.seek 0ml @22:57:24.66 Garaging FlushPuck in Collection Clamp @22:57:24.74 Elbow.reconfigure TightElbow @22:57:24.79 Elbow.seek CC @22:57:25.06 Elbow.reconfigure LooseElbow @22:57:25.16 Forearm.seek CC @22:57:26.21 Hand.close @22:57:26.87 CC.seek :open @22:57:31.25 Forearm.seek :retract @22:57:32.27 Elbow.reconfigure TightElbow @22:57:32.32 Elbow.seek :garage @22:57:35.36 Elbow.reconfigure LooseElbow @22:57:35.46 Forearm.reconfigure TightForearm @22:57:35.51 Forearm.seek :garage @22:57:36.71 Hand.open @22:57:37.44 Forearm.reconfigure LooseForearm @22:57:37.49 Forearm.seek :retract @22:57:38.52 Whole Cell Archive sampling 2000ml @22:57:38.54 Loading wcr puck from tube 2 into Collection Clamp @22:57:38.80 SC.reconfigure SCtight @22:57:38.85 SC.seek 2 @22:57:41.04 SC.reconfigure SCloose @22:57:41.28 Elbow.reconfigure TightElbow @22:57:41.33 Elbow.seek 2 @22:57:43.35 Forearm.reconfigure TightForearm @22:57:43.40 Forearm.seek 2 + 700 counts @22:57:44.98 SE.reconfigure SEpick @22:57:45.08 SE.seek beamBreak @22:57:47.18 SE.configure SEconfig @22:57:47.36 Elbow.seek 2 @22:57:47.66 Forearm.seek 2 + 300 counts @22:57:47.90 Forearm.reconfigure LooseForearm @22:57:47.95 Hand.close @22:57:48.64 SE.seek :down @22:57:48.88 Forearm.seek :retract @22:57:49.01 delay 3 seconds @22:57:49.84 Elbow.seek CC @22:57:50.62 SE.coast @22:57:51.55 Elbow.reconfigure LooseElbow @22:57:51.67 Forearm.reconfigure TightForearm @22:57:51.73 Forearm.seek CC @22:57:52.04 ATV.dial :waste2 @22:57:52.75 AS.reconfigure ASconfig @22:57:52.81 AS.seek :empty @22:57:53.14 CC.seek :closed @22:57:57.39 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.71 @22:57:57.40 Hand.open @22:57:58.06 Forearm.reconfigure LooseForearm @22:57:58.11 Forearm.seek :retract @22:57:58.47 ATV.dial :air2 @22:57:58.89 AS.reconfigure ASslow1 @22:57:58.94 AS.seek 1ml @22:57:59.15 SC.coast @22:57:59.19 collectSample 2000ml w/bubble point of 55psi @22:57:59.21 Priming 7ml @22:57:59.22 Intake.select :close @22:57:59.23 Exhaust.select :close @22:57:59.24 SS.hold @22:57:59.48 Calibrating pressure sensors @22:57:59.49 CTV.dial :air @22:58:00.78 SSV.dial :spit,avoiding: :exhaust @22:58:00.81 SS.reconfigure SSfast @22:58:00.85 SS.seek 5ml @22:58:03.46 SSV.dial CBV @22:58:03.50 CBV.dial SSV @22:58:04.87 delay 3.8 seconds @22:58:08.82 SSV.dial :spit @22:58:10.14 SS.seek 0ml @22:58:12.74 SSV.dial CBV @22:58:14.05 CTV.dial :intake @22:58:14.80 SSV.dial CBV @22:58:14.82 CBV.dial SSV @22:58:14.85 Allowing 40s for 7.0ml of remaining 7.0ml @22:58:14.86 Intake.select :open @22:58:17.04 Intake @32.0psia, vacuum from 20.0 to 24.0psi @22:58:17.06 SS.reconfigure SSinhale.with maxSpeed: 150 @22:58:17.10 SS.seek 7ml @22:58:22.46 Stroke completed -- waiting for pressure to equalize ... @22:58:32.15 Intake.select :close @22:58:32.31 SSV.dial :exhaust @22:58:33.09 Exhaust.select :open @22:58:33.21 SS.reconfigure SSexhale @22:58:33.26 SS.seek 0ml @22:58:37.02 Exhaust.select :close @22:58:37.13 Sampling 2000.0ml @22:58:37.15 Intake.select :close @22:58:37.16 Exhaust.select :close @22:58:37.17 SS.reconfigure SSconfig @22:58:37.22 SS.hold @22:58:37.44 CTV.dial :intake @22:58:37.46 SSV.dial CBV @22:58:37.50 CBV.dial SSV @22:58:38.28 Allowing 130s for 25.0ml of remaining 2000.0ml @22:58:38.29 Intake.select :open @22:58:40.48 Intake @31.8psia, vacuum from 20.0 to 24.0psi @22:58:40.50 SS.reconfigure SSinhale.with maxSpeed: 150 @22:58:40.55 SS.seek 25ml @22:58:44.53 Vacuum too high -- waiting up to 40s @22:58:46.67 Intake @31.7psia, vacuum from 20.0 to 24.0psi @22:58:46.69 SS.reconfigure SSinhale.with maxSpeed: 135 @22:58:46.74 SS.seek 25ml @22:58:50.07 Vacuum too high -- waiting up to 40s @22:58:52.22 Intake @31.9psia, vacuum from 20.0 to 24.0psi @22:58:52.24 SS.reconfigure SSinhale.with maxSpeed: 122 @22:58:52.29 SS.seek 25ml @22:58:55.50 Vacuum too high -- waiting up to 40s @22:58:57.40 Intake @32.1psia, vacuum from 20.0 to 24.0psi @22:58:57.42 SS.reconfigure SSinhale.with maxSpeed: 110 @22:58:57.46 SS.seek 25ml @22:59:00.51 Vacuum too high -- waiting up to 40s @22:59:02.20 Intake @31.6psia, vacuum from 20.0 to 24.0psi @22:59:02.22 SS.reconfigure SSinhale.with maxSpeed: 99 @22:59:02.26 SS.seek 25ml @22:59:05.68 Vacuum too high -- waiting up to 40s @22:59:07.56 Intake @31.7psia, vacuum from 20.0 to 24.0psi @22:59:07.58 SS.reconfigure SSinhale.with maxSpeed: 89 @22:59:07.62 SS.seek 25ml @22:59:11.40 Vacuum too high -- waiting up to 40s @22:59:13.03 Intake @32.1psia, vacuum from 20.0 to 24.0psi @22:59:13.05 SS.reconfigure SSinhale.with maxSpeed: 80 @22:59:13.10 SS.seek 25ml @22:59:14.98 Stroke completed -- waiting for pressure to equalize ... @22:59:17.58 Intake.select :close @22:59:17.74 SSV.dial :exhaust @22:59:18.51 Exhaust.select :open @22:59:18.62 SS.reconfigure SSexhale @22:59:18.68 SS.seek 0ml @22:59:27.39 Exhaust.select :close @22:59:27.55 SSV.dial CBV @22:59:27.57 CBV.dial SSV @22:59:28.37 Allowing 130s for 25.0ml of remaining 1975.0ml @22:59:28.39 Intake.select :open @22:59:30.57 Intake @32.2psia, vacuum from 20.0 to 24.0psi @22:59:30.59 SS.reconfigure SSinhale.with maxSpeed: 80 @22:59:30.64 SS.seek 25ml @22:59:36.24 Vacuum too high -- waiting up to 40s @22:59:37.61 Intake @32.0psia, vacuum from 20.0 to 24.0psi @22:59:37.63 SS.reconfigure SSinhale.with maxSpeed: 72 @22:59:37.67 SS.seek 25ml @22:59:43.17 Vacuum too high -- waiting up to 40s @22:59:44.57 SS.reconfigure SSinhale.with maxSpeed: 65 @22:59:44.62 SS.seek 25ml @22:59:53.61 Vacuum too high -- waiting up to 40s @22:59:54.99 Intake @31.2psia, vacuum from 20.0 to 24.0psi @22:59:55.01 SS.reconfigure SSinhale.with maxSpeed: 59 @22:59:55.06 SS.seek 25ml @23:00:05.14 Stroke completed -- waiting for pressure to equalize ... @23:00:08.41 Intake.select :close @23:00:08.57 SSV.dial :exhaust @23:00:09.35 Exhaust.select :open @23:00:09.47 SS.reconfigure SSexhale @23:00:09.52 SS.seek 0ml @23:00:18.18 Exhaust.select :close @23:00:18.30 SSV.dial CBV @23:00:18.32 CBV.dial SSV @23:00:19.12 Allowing 130s for 25.0ml of remaining 1950.0ml @23:00:19.14 Intake.select :open @23:00:21.32 Intake @31.7psia, vacuum from 20.0 to 24.0psi @23:00:21.34 SS.reconfigure SSinhale.with maxSpeed: 59 @23:00:21.39 SS.seek 25ml @23:00:44.50 Vacuum too high -- waiting up to 40s @23:00:45.88 Intake @31.3psia, vacuum from 20.0 to 24.0psi @23:00:45.89 SS.reconfigure SSinhale.with maxSpeed: 53 @23:00:45.94 SS.seek 25ml @23:00:54.32 Stroke completed -- waiting for pressure to equalize ... @23:00:57.54 Intake.select :close @23:00:57.71 SSV.dial :exhaust @23:00:58.48 Exhaust.select :open @23:00:58.60 SS.reconfigure SSexhale @23:00:58.65 SS.seek 0ml @23:01:07.66 Exhaust.select :close @23:01:07.78 SSV.dial CBV @23:01:07.80 CBV.dial SSV @23:01:08.58 Allowing 130s for 25.0ml of remaining 1925.0ml @23:01:08.59 Intake.select :open @23:01:10.78 Intake @32.1psia, vacuum from 20.0 to 24.0psi @23:01:10.80 SS.reconfigure SSinhale.with maxSpeed: 53 @23:01:10.85 SS.seek 25ml @23:01:20.52 delay 5 seconds @23:01:25.55 ATV.dial ASV @23:01:26.48 ASV.dial :SPEdelivery @23:01:26.50 AS.seek 0.02ml @23:01:44.43 Stroke completed -- waiting for pressure to equalize ... @23:01:47.68 Intake.select :close @23:01:47.84 SSV.dial :exhaust @23:01:48.63 Exhaust.select :open @23:01:48.75 SS.reconfigure SSexhale @23:01:48.80 SS.seek 0ml @23:01:57.66 Exhaust.select :close @23:01:57.78 SSV.dial CBV @23:01:57.80 CBV.dial SSV @23:01:58.58 Allowing 130s for 25.0ml of remaining 1900.0ml @23:01:58.59 Intake.select :open @23:02:00.78 Intake @32.0psia, vacuum from 20.0 to 24.0psi @23:02:00.80 SS.reconfigure SSinhale.with maxSpeed: 53 @23:02:00.85 SS.seek 25ml @23:02:34.90 Stroke completed -- waiting for pressure to equalize ... @23:02:38.07 Intake.select :close @23:02:38.23 SSV.dial :exhaust @23:02:39.01 Exhaust.select :open @23:02:39.14 SS.reconfigure SSexhale @23:02:39.19 SS.seek 0ml @23:02:48.10 Exhaust.select :close @23:02:48.22 SSV.dial CBV @23:02:48.24 CBV.dial SSV @23:02:49.04 Allowing 130s for 25.0ml of remaining 1875.0ml @23:02:49.06 Intake.select :open @23:02:51.23 Intake @31.7psia, vacuum from 20.0 to 24.0psi @23:02:51.25 SS.reconfigure SSinhale.with maxSpeed: 53 @23:02:51.34 SS.seek 25ml @23:03:25.19 Stroke completed -- waiting for pressure to equalize ... @23:03:28.42 Intake.select :close @23:03:28.58 SSV.dial :exhaust @23:03:29.37 Exhaust.select :open @23:03:29.48 SS.reconfigure SSexhale @23:03:29.54 SS.seek 0ml @23:03:38.26 Exhaust.select :close @23:03:38.38 SSV.dial CBV @23:03:38.40 CBV.dial SSV @23:03:39.19 Allowing 130s for 25.0ml of remaining 1850.0ml @23:03:39.21 Intake.select :open @23:03:41.39 Intake @32.2psia, vacuum from 20.0 to 24.0psi @23:03:41.41 SS.reconfigure SSinhale.with maxSpeed: 53 @23:03:41.46 SS.seek 25ml @23:04:15.44 Stroke completed -- waiting for pressure to equalize ... @23:04:19.33 Intake.select :close @23:04:19.50 SSV.dial :exhaust @23:04:20.27 Exhaust.select :open @23:04:20.38 SS.reconfigure SSexhale @23:04:20.44 SS.seek 0ml @23:04:29.29 Exhaust.select :close @23:04:29.41 SSV.dial CBV @23:04:29.43 CBV.dial SSV @23:04:30.21 Allowing 130s for 25.0ml of remaining 1825.0ml @23:04:30.22 Intake.select :open @23:04:32.40 Intake @31.8psia, vacuum from 20.0 to 24.0psi @23:04:32.42 SS.reconfigure SSinhale.with maxSpeed: 53 @23:04:32.47 SS.seek 25ml @23:04:43.86 delay 5 seconds @23:04:48.89 ATV.dial :air2 @23:04:49.90 AS.seek 0.52ml @23:05:06.16 Stroke completed -- waiting for pressure to equalize ... @23:05:09.41 Intake.select :close @23:05:09.57 SSV.dial :exhaust @23:05:10.35 Exhaust.select :open @23:05:10.47 SS.reconfigure SSexhale @23:05:10.52 SS.seek 0ml @23:05:19.13 Exhaust.select :close @23:05:19.25 SSV.dial CBV @23:05:19.27 CBV.dial SSV @23:05:20.07 Allowing 130s for 25.0ml of remaining 1800.0ml @23:05:20.08 Intake.select :open @23:05:22.27 Intake @32.3psia, vacuum from 20.0 to 24.0psi @23:05:22.29 SS.reconfigure SSinhale.with maxSpeed: 53 @23:05:22.36 SS.seek 25ml @23:05:25.30 BACfans.email "Started mfb in tube 2 at 20:49:33CEST11-Sep-19\n{1 => 9, 2 => 13}\nCan@20:45:23, 16.4C, 79% humidity, 14.6psia, 14.611V, 0.682A, 0.676A avg, 9.97W",Subject: "pcrBAC sampling at most 2000ml, wcr at most 2000ml" @23:05:25.88 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @23:05:25.90 Retry #5 of 10 after 10:00 @23:05:56.49 Stroke completed -- waiting for pressure to equalize ... @23:06:00.36 Intake.select :close @23:06:00.52 SSV.dial :exhaust @23:06:01.30 Exhaust.select :open @23:06:01.42 SS.reconfigure SSexhale @23:06:01.47 SS.seek 0ml @23:06:10.26 Exhaust.select :close @23:06:10.38 SSV.dial CBV @23:06:10.40 CBV.dial SSV @23:06:11.19 Allowing 130s for 25.0ml of remaining 1775.0ml @23:06:11.21 Intake.select :open @23:06:13.39 Intake @32.3psia, vacuum from 20.0 to 24.0psi @23:06:13.41 SS.reconfigure SSinhale.with maxSpeed: 53 @23:06:13.46 SS.seek 25ml @23:06:30.75 delay 5 seconds @23:06:35.78 ATV.dial ASV @23:06:36.72 ASV.dial :wasteR @23:06:37.09 AS.seek 0.3ml @23:06:47.29 Stroke completed -- waiting for pressure to equalize ... @23:06:51.17 Intake.select :close @23:06:51.33 SSV.dial :exhaust @23:06:52.10 Exhaust.select :open @23:06:52.22 SS.reconfigure SSexhale @23:06:52.27 SS.seek 0ml @23:07:01.15 Exhaust.select :close @23:07:01.27 SSV.dial CBV @23:07:01.29 CBV.dial SSV @23:07:02.08 Allowing 130s for 25.0ml of remaining 1750.0ml @23:07:02.09 Intake.select :open @23:07:04.28 Intake @32.2psia, vacuum from 20.0 to 24.0psi @23:07:04.30 SS.reconfigure SSinhale.with maxSpeed: 53 @23:07:04.34 SS.seek 25ml @23:07:21.63 delay 20 seconds @23:07:38.12 Stroke completed -- waiting for pressure to equalize ... @23:07:41.66 AS.seek 0.07ml @23:07:42.00 Intake.select :close @23:07:42.17 SSV.dial :exhaust @23:07:42.93 Exhaust.select :open @23:07:43.05 SS.reconfigure SSexhale @23:07:43.10 SS.seek 0ml @23:07:51.96 Exhaust.select :close @23:07:52.08 SSV.dial CBV @23:07:52.10 CBV.dial SSV @23:07:52.90 Allowing 130s for 25.0ml of remaining 1725.0ml @23:07:52.91 Intake.select :open @23:07:55.09 Intake @32.2psia, vacuum from 20.0 to 24.0psi @23:07:55.11 SS.reconfigure SSinhale.with maxSpeed: 53 @23:07:55.16 SS.seek 25ml @23:08:28.17 delay 20 seconds @23:08:28.90 Stroke completed -- waiting for pressure to equalize ... @23:08:33.35 Intake.select :close @23:08:33.52 SSV.dial :exhaust @23:08:34.30 Exhaust.select :open @23:08:34.42 SS.reconfigure SSexhale @23:08:34.47 SS.seek 0ml @23:08:43.15 Exhaust.select :close @23:08:43.27 SSV.dial CBV @23:08:43.29 CBV.dial SSV @23:08:44.08 Allowing 130s for 25.0ml of remaining 1700.0ml @23:08:44.09 Intake.select :open @23:08:46.28 Intake @31.4psia, vacuum from 20.0 to 24.0psi @23:08:46.30 SS.reconfigure SSinhale.with maxSpeed: 53 @23:08:46.35 SS.seek 25ml @23:08:48.20 ATV.dial :waste2 @23:08:49.03 AS.reconfigure ASconfig @23:08:49.08 AS.seek :empty @23:08:53.46 Elution @23:08:53.47 ATV.dial :air1 @23:08:54.56 AS.reconfigure ASslow1 @23:08:54.61 AS.seek 0.049ml @23:09:05.06 delay 3 seconds @23:09:08.09 ATV.dial :ElutionWater @23:09:08.75 AS.seek 0.109ml @23:09:20.41 Stroke completed -- waiting for pressure to equalize ... @23:09:21.08 delay 3 seconds @23:09:24.10 ATV.dial ASV,via: :air2 @23:09:24.33 Intake.select :close @23:09:24.50 SSV.dial :exhaust @23:09:25.27 Exhaust.select :open @23:09:25.39 SS.reconfigure SSexhale @23:09:25.45 SS.seek 0ml @23:09:26.05 ASV.dial :SPEelution @23:09:26.61 AFV.select :carrierSolution @23:09:26.83 AS.reconfigure ASnorm @23:09:26.89 AS.seek 0.759ml @23:09:34.15 Exhaust.select :close @23:09:34.27 SSV.dial CBV @23:09:34.29 CBV.dial SSV @23:09:35.10 Allowing 130s for 25.0ml of remaining 1675.0ml @23:09:35.12 Intake.select :open @23:09:37.30 Intake @32.3psia, vacuum from 20.0 to 24.0psi @23:09:37.32 SS.reconfigure SSinhale.with maxSpeed: 53 @23:09:37.37 SS.seek 25ml @23:09:59.92 delay 3 seconds @23:10:02.95 AFV.select :holdingCoil @23:10:03.11 AS.reconfigure ASslow1 @23:10:03.16 AS.seek 0.474ml @23:10:11.43 Stroke completed -- waiting for pressure to equalize ... @23:10:15.30 Intake.select :close @23:10:15.47 SSV.dial :exhaust @23:10:16.24 Exhaust.select :open @23:10:16.36 SS.reconfigure SSexhale @23:10:16.41 SS.seek 0ml @23:10:25.07 Exhaust.select :close @23:10:25.19 SSV.dial CBV @23:10:25.21 CBV.dial SSV @23:10:25.99 Allowing 130s for 25.0ml of remaining 1650.0ml @23:10:26.00 Intake.select :open @23:10:28.19 Intake @32.3psia, vacuum from 20.0 to 24.0psi @23:10:28.21 SS.reconfigure SSinhale.with maxSpeed: 53 @23:10:28.25 SS.seek 25ml @23:11:00.75 delay 5 seconds @23:11:02.51 Stroke completed -- waiting for pressure to equalize ... @23:11:05.84 AS.reconfigure ASslow2 @23:11:05.89 AS.seek 0.439ml @23:11:06.31 Intake.select :close @23:11:06.52 SSV.dial :exhaust @23:11:07.33 Exhaust.select :open @23:11:07.46 SS.reconfigure SSexhale @23:11:07.51 SS.seek 0ml @23:11:16.35 Exhaust.select :close @23:11:16.47 SSV.dial CBV @23:11:16.49 CBV.dial SSV @23:11:17.29 Allowing 130s for 25.0ml of remaining 1625.0ml @23:11:17.31 Intake.select :open @23:11:19.49 Intake @32.2psia, vacuum from 20.0 to 24.0psi @23:11:19.51 SS.reconfigure SSinhale.with maxSpeed: 53 @23:11:19.56 SS.seek 25ml @23:11:41.31 delay 1:00 @23:11:53.69 Stroke completed -- waiting for pressure to equalize ... @23:11:57.58 Intake.select :close @23:11:57.75 SSV.dial :exhaust @23:11:58.52 Exhaust.select :open @23:11:58.64 SS.reconfigure SSexhale @23:11:58.69 SS.seek 0ml @23:12:07.58 Exhaust.select :close @23:12:07.70 SSV.dial CBV @23:12:07.72 CBV.dial SSV @23:12:08.50 Allowing 130s for 25.0ml of remaining 1600.0ml @23:12:08.52 Intake.select :open @23:12:10.70 Intake @32.3psia, vacuum from 20.0 to 24.0psi @23:12:10.72 SS.reconfigure SSinhale.with maxSpeed: 53 @23:12:10.77 SS.seek 25ml @23:12:41.34 pushing eluate to holding coil @23:12:41.42 AS.seek 0.279ml @23:12:44.43 Stroke completed -- waiting for pressure to equalize ... @23:12:48.32 Intake.select :close @23:12:48.49 SSV.dial :exhaust @23:12:49.25 Exhaust.select :open @23:12:49.37 SS.reconfigure SSexhale @23:12:49.42 SS.seek 0ml @23:12:58.35 Exhaust.select :close @23:12:58.47 SSV.dial CBV @23:12:58.49 CBV.dial SSV @23:12:59.29 Allowing 130s for 25.0ml of remaining 1575.0ml @23:12:59.30 Intake.select :open @23:13:01.49 Intake @32.4psia, vacuum from 20.0 to 24.0psi @23:13:01.51 SS.reconfigure SSinhale.with maxSpeed: 53 @23:13:01.55 SS.seek 25ml @23:13:30.33 Vacuum too high -- waiting up to 40s @23:13:31.78 Intake @32.4psia, vacuum from 20.0 to 24.0psi @23:13:31.80 SS.reconfigure SSinhale.with maxSpeed: 48 @23:13:31.85 SS.seek 25ml @23:13:37.26 Stroke completed -- waiting for pressure to equalize ... @23:13:41.09 Intake.select :close @23:13:41.25 SSV.dial :exhaust @23:13:42.02 Exhaust.select :open @23:13:42.14 SS.reconfigure SSexhale @23:13:42.19 SS.seek 0ml @23:13:51.05 Exhaust.select :close @23:13:51.17 SSV.dial CBV @23:13:51.19 CBV.dial SSV @23:13:51.99 Allowing 130s for 25.0ml of remaining 1550.0ml @23:13:52.01 Intake.select :open @23:13:54.19 Intake @32.8psia, vacuum from 20.0 to 24.0psi @23:13:54.21 SS.reconfigure SSinhale.with maxSpeed: 48 @23:13:54.26 SS.seek 25ml @23:14:31.74 Stroke completed -- waiting for pressure to equalize ... @23:14:34.98 Intake.select :close @23:14:35.14 SSV.dial :exhaust @23:14:35.93 Exhaust.select :open @23:14:36.05 SS.reconfigure SSexhale @23:14:36.10 SS.seek 0ml @23:14:44.69 Exhaust.select :close @23:14:44.81 SSV.dial CBV @23:14:44.83 CBV.dial SSV @23:14:45.61 Allowing 130s for 25.0ml of remaining 1525.0ml @23:14:45.63 Intake.select :open @23:14:47.81 Intake @32.4psia, vacuum from 20.0 to 24.0psi @23:14:47.83 SS.reconfigure SSinhale.with maxSpeed: 48 @23:14:47.88 SS.seek 25ml @23:15:22.53 delay 30 seconds @23:15:25.32 Stroke completed -- waiting for pressure to equalize ... @23:15:25.97 BACfans.email "Started mfb in tube 2 at 20:49:33CEST11-Sep-19\n{1 => 9, 2 => 13}\nCan@20:45:23, 16.4C, 79% humidity, 14.6psia, 14.611V, 0.682A, 0.676A avg, 9.97W",Subject: "pcrBAC sampling at most 2000ml, wcr at most 2000ml" @23:15:26.01 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @23:15:26.02 Retry #6 of 10 after 10:00 @23:15:29.22 Intake.select :close @23:15:29.38 SSV.dial :exhaust @23:15:30.16 Exhaust.select :open @23:15:30.28 SS.reconfigure SSexhale @23:15:30.33 SS.seek 0ml @23:15:38.99 Exhaust.select :close @23:15:39.11 SSV.dial CBV @23:15:39.13 CBV.dial SSV @23:15:39.93 Allowing 130s for 25.0ml of remaining 1500.0ml @23:15:39.95 Intake.select :open @23:15:42.13 Intake @32.9psia, vacuum from 20.0 to 24.0psi @23:15:42.15 SS.reconfigure SSinhale.with maxSpeed: 48 @23:15:42.19 SS.seek 25ml @23:15:52.56 ATV.dial :waste,via: :air3 @23:15:54.13 AS.reconfigure ASconfig @23:15:54.18 AS.seek :empty @23:16:10.56 Parking Bleach in LysateSource line and SPEcolumn @23:16:10.57 ATV.dial :air3 @23:16:11.46 AS.reconfigure ASslow1 @23:16:11.51 AS.seek 0.029ml @23:16:17.88 ATV.dial :ElutionWater @23:16:18.46 AS.seek 0.059ml @23:16:19.66 Stroke completed -- waiting for pressure to equalize ... @23:16:23.53 Intake.select :close @23:16:23.69 SSV.dial :exhaust @23:16:24.46 Exhaust.select :open @23:16:24.58 SS.reconfigure SSexhale @23:16:25.20 ATV.dial :air3 @23:16:25.29 SS.seek 0ml @23:16:25.84 AS.seek 0.069ml @23:16:28.20 delay 2 seconds @23:16:30.23 ATV.dial :bleach @23:16:30.68 AS.seek 0.769ml @23:16:34.07 Exhaust.select :close @23:16:34.19 SSV.dial CBV @23:16:34.21 CBV.dial SSV @23:16:35.00 Allowing 130s for 25.0ml of remaining 1475.0ml @23:16:35.02 Intake.select :open @23:16:37.20 Intake @32.7psia, vacuum from 20.0 to 24.0psi @23:16:37.22 SS.reconfigure SSinhale.with maxSpeed: 48 @23:16:37.27 SS.seek 25ml @23:17:14.75 Stroke completed -- waiting for pressure to equalize ... @23:17:18.63 Intake.select :close @23:17:18.79 SSV.dial :exhaust @23:17:19.57 Exhaust.select :open @23:17:19.69 SS.reconfigure SSexhale @23:17:19.74 SS.seek 0ml @23:17:28.44 Exhaust.select :close @23:17:28.55 SSV.dial CBV @23:17:28.58 CBV.dial SSV @23:17:29.36 Allowing 130s for 25.0ml of remaining 1450.0ml @23:17:29.37 Intake.select :open @23:17:31.55 Intake @32.3psia, vacuum from 20.0 to 24.0psi @23:17:31.57 SS.reconfigure SSinhale.with maxSpeed: 48 @23:17:31.62 SS.seek 25ml @23:18:09.00 Stroke completed -- waiting for pressure to equalize ... @23:18:12.89 Intake.select :close @23:18:13.06 SSV.dial :exhaust @23:18:13.83 Exhaust.select :open @23:18:13.95 SS.reconfigure SSexhale @23:18:14.00 SS.seek 0ml @23:18:22.83 Exhaust.select :close @23:18:22.95 SSV.dial CBV @23:18:22.97 CBV.dial SSV @23:18:23.75 Allowing 130s for 25.0ml of remaining 1425.0ml @23:18:23.76 Intake.select :open @23:18:25.95 Intake @32.2psia, vacuum from 20.0 to 24.0psi @23:18:25.97 SS.reconfigure SSinhale.with maxSpeed: 48 @23:18:26.02 SS.seek 25ml @23:18:51.71 delay 3 seconds @23:18:54.74 ATV.dial :air3 @23:18:55.27 delay 3 seconds @23:18:58.36 AS.seek 0.794ml @23:19:03.03 Stroke completed -- waiting for pressure to equalize ... @23:19:03.65 delay 3 seconds @23:19:06.68 ATV.dial :bleach @23:19:06.97 Intake.select :close @23:19:07.14 SSV.dial :exhaust @23:19:07.28 AS.seek 0.779ml @23:19:07.89 Exhaust.select :open @23:19:08.01 SS.reconfigure SSexhale @23:19:08.06 SS.seek 0ml @23:19:10.60 delay 3 seconds @23:19:13.63 ATV.dial :AIV @23:19:14.89 AS.reconfigure ASconfig @23:19:14.95 AS.seek 0.015ml @23:19:16.69 Exhaust.select :close @23:19:16.81 SSV.dial CBV @23:19:16.83 CBV.dial SSV @23:19:17.63 Allowing 130s for 25.0ml of remaining 1400.0ml @23:19:17.65 Intake.select :open @23:19:19.83 Intake @33.1psia, vacuum from 20.0 to 24.0psi @23:19:19.85 SS.reconfigure SSinhale.with maxSpeed: 48 @23:19:19.90 SS.seek 25ml @23:19:57.32 Stroke completed -- waiting for pressure to equalize ... @23:19:59.19 ATV.dial :waste @23:20:00.64 AS.seek :empty @23:20:01.27 Intake.select :close @23:20:01.44 SSV.dial :exhaust @23:20:01.85 ATV.dial :air3 @23:20:02.22 Exhaust.select :open @23:20:02.34 SS.reconfigure SSexhale @23:20:02.43 SS.seek 0ml @23:20:02.58 AS.reconfigure ASslow1 @23:20:02.63 AS.seek 0.009ml @23:20:04.96 delay 2 seconds @23:20:06.98 ATV.dial :bleach @23:20:07.44 AS.seek 0.579ml @23:20:11.38 Exhaust.select :close @23:20:11.50 SSV.dial CBV @23:20:11.52 CBV.dial SSV @23:20:12.32 Allowing 130s for 25.0ml of remaining 1375.0ml @23:20:12.33 Intake.select :open @23:20:14.53 Intake @32.6psia, vacuum from 20.0 to 24.0psi @23:20:14.55 SS.reconfigure SSinhale.with maxSpeed: 48 @23:20:14.60 SS.seek 25ml @23:20:51.85 Stroke completed -- waiting for pressure to equalize ... @23:20:56.37 Intake.select :close @23:20:56.53 SSV.dial :exhaust @23:20:57.31 Exhaust.select :open @23:20:57.44 SS.reconfigure SSexhale @23:20:57.49 SS.seek 0ml @23:21:06.36 Exhaust.select :close @23:21:06.48 SSV.dial CBV @23:21:06.50 CBV.dial SSV @23:21:07.28 Allowing 130s for 25.0ml of remaining 1350.0ml @23:21:07.30 Intake.select :open @23:21:09.48 Intake @31.9psia, vacuum from 20.0 to 24.0psi @23:21:09.50 SS.reconfigure SSinhale.with maxSpeed: 48 @23:21:09.54 SS.seek 25ml @23:21:46.93 Stroke completed -- waiting for pressure to equalize ... @23:21:51.46 Intake.select :close @23:21:51.62 SSV.dial :exhaust @23:21:52.40 Exhaust.select :open @23:21:52.52 SS.reconfigure SSexhale @23:21:52.58 SS.seek 0ml @23:22:01.58 Exhaust.select :close @23:22:02.27 SSV.dial CBV @23:22:02.29 CBV.dial SSV @23:22:02.37 delay 3 seconds @23:22:03.11 Allowing 130s for 25.0ml of remaining 1325.0ml @23:22:03.13 Intake.select :open @23:22:05.31 Intake @32.6psia, vacuum from 20.0 to 24.0psi @23:22:05.33 SS.reconfigure SSinhale.with maxSpeed: 48 @23:22:05.38 SS.seek 25ml @23:22:05.43 ATV.dial :air3 @23:22:05.96 delay 3 seconds @23:22:09.05 AS.seek 0.604ml @23:22:14.36 delay 3 seconds @23:22:17.39 ATV.dial :bleach @23:22:17.98 AS.seek 0.589ml @23:22:21.27 delay 3 seconds @23:22:24.30 ATV.dial :ASV @23:22:25.28 ASV.dial :wasteR @23:22:25.86 AS.seek 0ml @23:22:42.44 Stroke completed -- waiting for pressure to equalize ... @23:22:47.02 Intake.select :close @23:22:47.19 SSV.dial :exhaust @23:22:47.97 Exhaust.select :open @23:22:48.09 SS.reconfigure SSexhale @23:22:48.14 SS.seek 0ml @23:22:56.83 Exhaust.select :close @23:22:56.95 SSV.dial CBV @23:22:56.97 CBV.dial SSV @23:22:57.77 Allowing 130s for 25.0ml of remaining 1300.0ml @23:22:57.79 Intake.select :open @23:22:59.97 Intake @32.8psia, vacuum from 20.0 to 24.0psi @23:22:59.99 SS.reconfigure SSinhale.with maxSpeed: 48 @23:23:00.04 SS.seek 25ml @23:23:37.42 Stroke completed -- waiting for pressure to equalize ... @23:23:41.93 Intake.select :close @23:23:42.09 SSV.dial :exhaust @23:23:42.86 Exhaust.select :open @23:23:42.98 SS.reconfigure SSexhale @23:23:43.03 SS.seek 0ml @23:23:51.67 Exhaust.select :close @23:23:51.79 SSV.dial CBV @23:23:51.81 CBV.dial SSV @23:23:52.61 Allowing 130s for 25.0ml of remaining 1275.0ml @23:23:52.63 Intake.select :open @23:23:54.81 Intake @32.6psia, vacuum from 20.0 to 24.0psi @23:23:54.83 SS.reconfigure SSinhale.with maxSpeed: 48 @23:23:54.88 SS.seek 25ml @23:24:24.64 delay 1:00 @23:24:32.26 Stroke completed -- waiting for pressure to equalize ... @23:24:36.95 Intake.select :close @23:24:37.11 SSV.dial :exhaust @23:24:37.89 Exhaust.select :open @23:24:38.01 SS.reconfigure SSexhale @23:24:38.06 SS.seek 0ml @23:24:47.03 Exhaust.select :close @23:24:47.15 SSV.dial CBV @23:24:47.18 CBV.dial SSV @23:24:47.96 Allowing 130s for 25.0ml of remaining 1250.0ml @23:24:47.98 Intake.select :open @23:24:50.16 Intake @31.9psia, vacuum from 20.0 to 24.0psi @23:24:50.18 SS.reconfigure SSinhale.with maxSpeed: 48 @23:24:50.23 SS.seek 25ml @23:25:24.67 AFV.select :carrierSolution @23:25:24.77 delay 2 seconds @23:25:26.05 BACfans.email "Started mfb in tube 2 at 20:49:33CEST11-Sep-19\n{1 => 9, 2 => 13}\nCan@20:45:23, 16.4C, 79% humidity, 14.6psia, 14.611V, 0.682A, 0.676A avg, 9.97W",Subject: "pcrBAC sampling at most 2000ml, wcr at most 2000ml" @23:25:26.08 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @23:25:26.10 Retry #7 of 10 after 10:00 @23:25:26.80 AS.reconfigure ASconfig @23:25:26.85 AS.seek 1ml @23:25:27.35 Stroke completed -- waiting for pressure to equalize ... @23:25:32.51 Intake.select :close @23:25:32.67 SSV.dial :exhaust @23:25:33.46 Exhaust.select :open @23:25:33.58 SS.reconfigure SSexhale @23:25:33.63 SS.seek 0ml @23:25:42.67 Exhaust.select :close @23:25:42.79 SSV.dial CBV @23:25:42.81 CBV.dial SSV @23:25:43.60 Allowing 130s for 25.0ml of remaining 1225.0ml @23:25:43.62 Intake.select :open @23:25:45.80 Intake @32.9psia, vacuum from 20.0 to 24.0psi @23:25:45.82 SS.reconfigure SSinhale.with maxSpeed: 48 @23:25:45.87 SS.seek 25ml @23:26:10.09 Vacuum too high -- waiting up to 40s @23:26:11.47 Intake @32.5psia, vacuum from 20.0 to 24.0psi @23:26:11.49 SS.reconfigure SSinhale.with maxSpeed: 43 @23:26:11.53 SS.seek 25ml @23:26:24.61 delay 1 second @23:26:25.63 AFV.select :holdingCoil @23:26:25.73 delay 1 second @23:26:25.91 Stroke completed -- waiting for pressure to equalize ... @23:26:26.75 ATV.dial ASV @23:26:26.76 ASV.dial :wasteR @23:26:26.78 AS.reconfigure ASslow1 @23:26:26.83 AS.seek 0ml @23:26:29.15 Intake.select :close @23:26:29.31 SSV.dial :exhaust @23:26:30.11 Exhaust.select :open @23:26:30.22 SS.reconfigure SSexhale @23:26:30.28 SS.seek 0ml @23:26:39.14 Exhaust.select :close @23:26:39.26 SSV.dial CBV @23:26:39.28 CBV.dial SSV @23:26:40.06 Allowing 130s for 25.0ml of remaining 1200.0ml @23:26:40.08 Intake.select :open @23:26:42.26 Intake @32.7psia, vacuum from 20.0 to 24.0psi @23:26:42.28 SS.reconfigure SSinhale.with maxSpeed: 43 @23:26:42.33 SS.seek 25ml @23:27:23.69 Stroke completed -- waiting for pressure to equalize ... @23:27:27.56 Intake.select :close @23:27:28.29 SSV.dial :exhaust @23:27:29.06 Exhaust.select :open @23:27:29.19 SS.reconfigure SSexhale @23:27:29.24 SS.seek 0ml @23:27:38.14 Exhaust.select :close @23:27:38.26 SSV.dial CBV @23:27:38.28 CBV.dial SSV @23:27:39.08 Allowing 130s for 25.0ml of remaining 1175.0ml @23:27:39.10 Intake.select :open @23:27:41.27 Intake @32.7psia, vacuum from 20.0 to 24.0psi @23:27:41.29 SS.reconfigure SSinhale.with maxSpeed: 43 @23:27:41.35 SS.seek 25ml @23:28:22.64 Stroke completed -- waiting for pressure to equalize ... @23:28:26.51 Intake.select :close @23:28:26.67 SSV.dial :exhaust @23:28:27.46 Exhaust.select :open @23:28:27.58 SS.reconfigure SSexhale @23:28:27.63 SS.seek 0ml @23:28:36.49 Exhaust.select :close @23:28:36.61 SSV.dial CBV @23:28:36.63 CBV.dial SSV @23:28:37.42 Allowing 130s for 25.0ml of remaining 1150.0ml @23:28:37.44 Intake.select :open @23:28:39.62 Intake @33.1psia, vacuum from 20.0 to 24.0psi @23:28:39.64 SS.reconfigure SSinhale.with maxSpeed: 43 @23:28:39.69 SS.seek 25ml @23:29:20.97 Stroke completed -- waiting for pressure to equalize ... @23:29:25.54 Intake.select :close @23:29:25.70 SSV.dial :exhaust @23:29:26.46 Exhaust.select :open @23:29:26.58 SS.reconfigure SSexhale @23:29:26.64 SS.seek 0ml @23:29:35.60 Exhaust.select :close @23:29:35.72 SSV.dial CBV @23:29:35.74 CBV.dial SSV @23:29:36.53 Allowing 130s for 25.0ml of remaining 1125.0ml @23:29:36.55 Intake.select :open @23:29:38.73 Intake @32.8psia, vacuum from 20.0 to 24.0psi @23:29:38.75 SS.reconfigure SSinhale.with maxSpeed: 43 @23:29:38.79 SS.seek 25ml @23:29:48.23 delay 20 seconds @23:30:08.26 ATV.dial :air3 @23:30:09.67 AS.seek 0.59ml @23:30:20.08 Stroke completed -- waiting for pressure to equalize ... @23:30:24.59 Intake.select :close @23:30:24.75 SSV.dial :exhaust @23:30:25.53 Exhaust.select :open @23:30:25.65 SS.reconfigure SSexhale @23:30:25.70 SS.seek 0ml @23:30:34.42 Exhaust.select :close @23:30:34.54 SSV.dial CBV @23:30:34.56 CBV.dial SSV @23:30:35.35 Allowing 130s for 25.0ml of remaining 1100.0ml @23:30:35.37 Intake.select :open @23:30:38.11 Intake @33.2psia, vacuum from 20.0 to 24.0psi @23:30:38.13 SS.reconfigure SSinhale.with maxSpeed: 43 @23:30:38.18 SS.seek 25ml @23:31:19.81 Stroke completed -- waiting for pressure to equalize ... @23:31:24.31 Intake.select :close @23:31:24.47 SSV.dial :exhaust @23:31:25.26 Exhaust.select :open @23:31:25.38 SS.reconfigure SSexhale @23:31:25.43 SS.seek 0ml @23:31:34.22 Exhaust.select :close @23:31:34.38 SSV.dial CBV @23:31:34.41 CBV.dial SSV @23:31:35.20 Allowing 130s for 25.0ml of remaining 1075.0ml @23:31:35.21 Intake.select :open @23:31:37.40 Intake @33.0psia, vacuum from 20.0 to 24.0psi @23:31:37.42 SS.reconfigure SSinhale.with maxSpeed: 43 @23:31:37.47 SS.seek 25ml @23:32:08.63 delay 3 seconds @23:32:11.66 ATV.dial :ASV @23:32:12.97 ASV.dial :wasteR @23:32:13.06 AS.seek 0.09ml @23:32:19.00 Stroke completed -- waiting for pressure to equalize ... @23:32:23.53 Intake.select :close @23:32:23.69 SSV.dial :exhaust @23:32:24.48 Exhaust.select :open @23:32:24.59 SS.reconfigure SSexhale @23:32:24.65 SS.seek 0ml @23:32:33.22 Exhaust.select :close @23:32:33.34 SSV.dial CBV @23:32:33.36 CBV.dial SSV @23:32:34.16 Allowing 130s for 25.0ml of remaining 1050.0ml @23:32:34.18 Intake.select :open @23:32:36.36 Intake @32.4psia, vacuum from 20.0 to 24.0psi @23:32:36.38 SS.reconfigure SSinhale.with maxSpeed: 43 @23:32:36.42 SS.seek 25ml @23:33:18.05 Stroke completed -- waiting for pressure to equalize ... @23:33:22.55 Intake.select :close @23:33:22.72 SSV.dial :exhaust @23:33:23.50 Exhaust.select :open @23:33:23.62 SS.reconfigure SSexhale @23:33:23.66 SS.seek 0ml @23:33:32.44 Exhaust.select :close @23:33:32.55 SSV.dial CBV @23:33:32.58 CBV.dial SSV @23:33:33.36 Allowing 130s for 25.0ml of remaining 1025.0ml @23:33:33.37 Intake.select :open @23:33:35.55 Intake @32.7psia, vacuum from 20.0 to 24.0psi @23:33:35.57 SS.reconfigure SSinhale.with maxSpeed: 43 @23:33:35.62 SS.seek 25ml @23:33:53.83 delay 30 seconds @23:34:17.27 Stroke completed -- waiting for pressure to equalize ... @23:34:22.36 Intake.select :close @23:34:22.52 SSV.dial :exhaust @23:34:23.30 Exhaust.select :open @23:34:23.42 SS.reconfigure SSexhale @23:34:23.47 SS.seek 0ml @23:34:23.85 ATV.dial :extSrc @23:34:24.68 delay 3 seconds @23:34:27.71 ATV.dial :ElutionWater @23:34:28.76 AS.seek 0.12ml @23:34:32.31 Exhaust.select :close @23:34:32.43 SSV.dial CBV @23:34:32.45 CBV.dial SSV @23:34:33.25 Allowing 130s for 25.0ml of remaining 1000.0ml @23:34:33.27 Intake.select :open @23:34:35.05 ATV.dial :air3 @23:34:35.45 Intake @33.1psia, vacuum from 20.0 to 24.0psi @23:34:35.47 SS.reconfigure SSinhale.with maxSpeed: 43 @23:34:35.52 SS.seek 25ml @23:34:35.68 AS.seek 0.13ml @23:34:37.96 delay 2 seconds @23:34:39.99 ATV.dial :bleach @23:34:40.43 AS.seek 0.23ml @23:35:00.83 delay 3 seconds @23:35:03.86 ATV.dial :air3 @23:35:04.38 delay 3 seconds @23:35:07.47 AS.seek 0.255ml @23:35:12.77 delay 3 seconds @23:35:15.80 ATV.dial :bleach @23:35:16.39 AS.seek 0.24ml @23:35:17.09 Stroke completed -- waiting for pressure to equalize ... @23:35:19.71 delay 3 seconds @23:35:21.61 Intake.select :close @23:35:21.78 SSV.dial :exhaust @23:35:22.56 Exhaust.select :open @23:35:22.68 SS.reconfigure SSexhale @23:35:22.73 SS.seek 0ml @23:35:22.77 ATV.dial :ASV @23:35:23.75 ASV.dial :SPEdelivery @23:35:24.11 AFV.select :carrierSolution @23:35:24.22 delay 2 seconds @23:35:26.12 BACfans.email "Started mfb in tube 2 at 20:49:33CEST11-Sep-19\n{1 => 9, 2 => 13}\nCan@20:45:23, 16.4C, 79% humidity, 14.6psia, 14.611V, 0.682A, 0.676A avg, 9.97W",Subject: "pcrBAC sampling at most 2000ml, wcr at most 2000ml" @23:35:26.15 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @23:35:26.16 Retry #8 of 10 after 10:00 @23:35:26.24 AS.reconfigure ASconfig @23:35:26.29 AS.seek 0.5ml @23:35:31.58 Exhaust.select :close @23:35:31.70 SSV.dial CBV @23:35:31.72 CBV.dial SSV @23:35:32.52 Allowing 130s for 25.0ml of remaining 975.0ml @23:35:32.53 Intake.select :open @23:35:34.72 Intake @32.7psia, vacuum from 20.0 to 24.0psi @23:35:34.74 SS.reconfigure SSinhale.with maxSpeed: 43 @23:35:34.78 SS.seek 25ml @23:35:41.55 delay 1 second @23:35:42.57 AFV.select :holdingCoil @23:35:42.72 AS.reconfigure ASslow1 @23:35:42.78 AS.seek 0.17ml @23:36:16.20 Stroke completed -- waiting for pressure to equalize ... @23:36:21.92 Intake.select :close @23:36:22.08 SSV.dial :exhaust @23:36:22.86 Exhaust.select :open @23:36:22.98 SS.reconfigure SSexhale @23:36:23.03 SS.seek 0ml @23:36:31.91 Exhaust.select :close @23:36:32.03 SSV.dial CBV @23:36:32.05 CBV.dial SSV @23:36:32.84 Allowing 130s for 25.0ml of remaining 950.0ml @23:36:32.85 Intake.select :open @23:36:35.03 Intake @32.8psia, vacuum from 20.0 to 24.0psi @23:36:35.05 SS.reconfigure SSinhale.with maxSpeed: 43 @23:36:35.10 SS.seek 25ml @23:36:49.42 SPE.hold 2:46:40,95C @23:36:49.48 delay 3:00 @23:37:16.38 Stroke completed -- waiting for pressure to equalize ... @23:37:21.56 Intake.select :close @23:37:21.72 SSV.dial :exhaust @23:37:22.52 Exhaust.select :open @23:37:22.65 SS.reconfigure SSexhale @23:37:22.70 SS.seek 0ml @23:37:31.50 Exhaust.select :close @23:37:31.62 SSV.dial CBV @23:37:31.64 CBV.dial SSV @23:37:32.47 Allowing 130s for 25.0ml of remaining 925.0ml @23:37:32.49 Intake.select :open @23:37:34.67 Intake @32.6psia, vacuum from 20.0 to 24.0psi @23:37:34.69 SS.reconfigure SSinhale.with maxSpeed: 43 @23:37:34.74 SS.seek 25ml @23:38:16.06 Stroke completed -- waiting for pressure to equalize ... @23:38:21.23 Intake.select :close @23:38:21.39 SSV.dial :exhaust @23:38:22.17 Exhaust.select :open @23:38:22.29 SS.reconfigure SSexhale @23:38:22.34 SS.seek 0ml @23:38:31.27 Exhaust.select :close @23:38:31.39 SSV.dial CBV @23:38:31.41 CBV.dial SSV @23:38:32.22 Allowing 130s for 25.0ml of remaining 900.0ml @23:38:32.24 Intake.select :open @23:38:34.42 Intake @33.5psia, vacuum from 20.0 to 24.0psi @23:38:34.44 SS.reconfigure SSinhale.with maxSpeed: 43 @23:38:34.49 SS.seek 25ml @23:38:59.08 Vacuum too high -- waiting up to 40s @23:39:00.20 Intake @32.2psia, vacuum from 20.0 to 24.0psi @23:39:00.22 SS.reconfigure SSinhale.with maxSpeed: 39 @23:39:00.26 SS.seek 25ml @23:39:18.61 Stroke completed -- waiting for pressure to equalize ... @23:39:22.43 Intake.select :close @23:39:22.59 SSV.dial :exhaust @23:39:23.39 Exhaust.select :open @23:39:23.52 SS.reconfigure SSexhale @23:39:23.57 SS.seek 0ml @23:39:32.36 Exhaust.select :close @23:39:32.48 SSV.dial CBV @23:39:32.50 CBV.dial SSV @23:39:33.31 Allowing 130s for 25.0ml of remaining 875.0ml @23:39:33.33 Intake.select :open @23:39:35.52 Intake @33.5psia, vacuum from 20.0 to 24.0psi @23:39:35.54 SS.reconfigure SSinhale.with maxSpeed: 39 @23:39:35.59 SS.seek 25ml @23:39:49.53 AFV.select :carrierSolution @23:39:49.63 delay 2 seconds @23:39:51.66 AS.reconfigure ASconfig @23:39:51.71 AS.seek 1ml @23:40:21.25 Stroke completed -- waiting for pressure to equalize ... @23:40:25.81 Intake.select :close @23:40:25.98 SSV.dial :exhaust @23:40:26.78 Exhaust.select :open @23:40:26.90 SS.reconfigure SSexhale @23:40:26.95 SS.seek 0ml @23:40:35.89 Exhaust.select :close @23:40:36.01 SSV.dial CBV @23:40:36.03 CBV.dial SSV @23:40:36.83 Allowing 130s for 25.0ml of remaining 850.0ml @23:40:36.84 Intake.select :open @23:40:39.03 Intake @33.0psia, vacuum from 20.0 to 24.0psi @23:40:39.05 SS.reconfigure SSinhale.with maxSpeed: 39 @23:40:39.10 SS.seek 25ml @23:40:39.69 delay 1 second @23:40:40.71 AFV.select :holdingCoil @23:40:40.81 delay 1 second @23:40:41.84 ASV.dial :wasteD @23:40:42.80 AS.seek 0ml @23:41:24.31 Stroke completed -- waiting for pressure to equalize ... @23:41:28.82 Intake.select :close @23:41:28.98 SSV.dial :exhaust @23:41:29.77 Exhaust.select :open @23:41:29.89 SS.reconfigure SSexhale @23:41:29.94 SS.seek 0ml @23:41:38.91 Exhaust.select :close @23:41:39.03 SSV.dial CBV @23:41:39.05 CBV.dial SSV @23:41:39.86 Allowing 130s for 25.0ml of remaining 825.0ml @23:41:39.87 Intake.select :open @23:41:40.58 Priming Eluate @23:41:40.59 AFV.select :carrierSolution @23:41:40.70 delay 2 seconds @23:41:42.06 Intake @33.0psia, vacuum from 20.0 to 24.0psi @23:41:42.08 SS.reconfigure SSinhale.with maxSpeed: 39 @23:41:42.13 SS.seek 25ml @23:41:42.73 AS.seek 0.5ml @23:42:11.80 delay 1 second @23:42:12.82 AFV.select :holdingCoil @23:42:12.92 ATV.dial ASV @23:42:12.93 ASV.dial :SPErecovery @23:42:13.49 Swish #1 of 2 @23:42:13.57 AS.reconfigure ASslow0 @23:42:13.62 AS.seek 0.6ml @23:42:24.03 AS.seek 0.5ml @23:42:27.66 Stroke completed -- waiting for pressure to equalize ... @23:42:32.77 Intake.select :close @23:42:32.94 SSV.dial :exhaust @23:42:33.72 Exhaust.select :open @23:42:33.85 SS.reconfigure SSexhale @23:42:33.90 SS.seek 0ml @23:42:34.41 Swish #2 of 2 @23:42:34.50 AS.seek 0.6ml @23:42:43.03 Exhaust.select :close @23:42:43.14 SSV.dial CBV @23:42:43.17 CBV.dial SSV @23:42:43.95 Allowing 130s for 25.0ml of remaining 800.0ml @23:42:43.97 Intake.select :open @23:42:44.98 AS.seek 0.5ml @23:42:46.15 Intake @33.3psia, vacuum from 20.0 to 24.0psi @23:42:46.17 SS.reconfigure SSinhale.with maxSpeed: 39 @23:42:46.22 SS.seek 25ml @23:42:55.36 delay 3 seconds @23:42:58.45 AS.reconfigure ASslow1 @23:42:58.50 AS.seek 0.684ml @23:43:31.20 Stroke completed -- waiting for pressure to equalize ... @23:43:35.80 delay 3 seconds @23:43:36.34 Intake.select :close @23:43:36.50 SSV.dial :exhaust @23:43:37.28 Exhaust.select :open @23:43:37.40 SS.reconfigure SSexhale @23:43:37.45 SS.seek 0ml @23:43:38.82 Eluate primed @23:43:38.83 ATV.dial :waste2 @23:43:39.41 AS.reconfigure ASnorm @23:43:39.47 AS.seek 0ml @23:43:46.16 Exhaust.select :close @23:43:46.28 SSV.dial CBV @23:43:46.30 CBV.dial SSV @23:43:47.09 Allowing 130s for 25.0ml of remaining 775.0ml @23:43:47.10 Intake.select :open @23:43:49.29 Intake @33.4psia, vacuum from 20.0 to 24.0psi @23:43:49.31 SS.reconfigure SSinhale.with maxSpeed: 39 @23:43:49.36 SS.seek 25ml @23:44:14.29 SPE.coast @23:44:14.37 ASV.dial :SPErecovery @23:44:14.39 Setting up PCR for Pp -- writing /var/log/esp/Pp19sep1123h1450ml.pcr @23:44:14.41 Building PCR Slug from ASV with MM1 mix, PP2 primer @23:44:14.42 Priming with carrier @23:44:14.43 ATV.dial ARV @23:44:15.46 ARV.dial :carrier @23:44:15.82 AS.seek 0.5ml @23:44:35.07 Stroke completed -- waiting for pressure to equalize ... @23:44:40.23 Intake.select :close @23:44:40.40 SSV.dial :exhaust @23:44:41.17 Exhaust.select :open @23:44:41.29 SS.reconfigure SSexhale @23:44:41.34 SS.seek 0ml @23:44:41.43 AFV.select :carrierSolution @23:44:41.57 delay 2 seconds @23:44:43.60 AS.reconfigure ASconfig @23:44:43.65 AS.seek 1ml @23:44:50.08 Exhaust.select :close @23:44:50.20 SSV.dial CBV @23:44:50.22 CBV.dial SSV @23:44:51.01 Allowing 130s for 25.0ml of remaining 750.0ml @23:44:51.03 Intake.select :open @23:44:53.21 Intake @33.0psia, vacuum from 20.0 to 24.0psi @23:44:53.23 SS.reconfigure SSinhale.with maxSpeed: 39 @23:44:53.28 SS.seek 25ml @23:45:13.30 delay 1 second @23:45:14.33 AFV.select :holdingCoil @23:45:14.43 delay 1 second @23:45:15.46 ATV.dial :waste @23:45:16.89 AS.reconfigure ASnorm @23:45:16.94 AS.seek :empty @23:45:26.19 BACfans.email "Started mfb in tube 2 at 20:49:33CEST11-Sep-19\n{1 => 9, 2 => 13}\nCan@20:45:23, 16.4C, 79% humidity, 14.6psia, 14.611V, 0.682A, 0.676A avg, 9.97W",Subject: "pcrBAC sampling at most 2000ml, wcr at most 2000ml" @23:45:26.22 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @23:45:26.24 Retry #9 of 10 after 10:00 @23:45:38.89 Stroke completed -- waiting for pressure to equalize ... @23:45:44.68 Intake.select :close @23:45:44.84 SSV.dial :exhaust @23:45:45.62 Exhaust.select :open @23:45:45.74 SS.reconfigure SSexhale @23:45:45.79 SS.seek 0ml @23:45:54.48 Exhaust.select :close @23:45:54.61 SSV.dial CBV @23:45:54.63 CBV.dial SSV @23:45:55.42 Allowing 130s for 25.0ml of remaining 725.0ml @23:45:55.44 Intake.select :open @23:45:57.61 Intake @32.9psia, vacuum from 20.0 to 24.0psi @23:45:57.63 SS.reconfigure SSinhale.with maxSpeed: 39 @23:45:57.68 SS.seek 25ml @23:46:07.69 AFV.select :carrierSolution @23:46:07.80 delay 2 seconds @23:46:09.83 AS.reconfigure ASconfig @23:46:09.88 AS.seek 0.5ml @23:46:38.97 delay 1 second @23:46:40.00 AFV.select :holdingCoil @23:46:40.10 Building Slug @23:46:40.11 ATV.dial :ARV @23:46:41.63 delay 2 seconds @23:46:43.44 Stroke completed -- waiting for pressure to equalize ... @23:46:43.66 ARV.dial :air1 @23:46:44.06 delay 2 seconds @23:46:46.15 AS.reconfigure ASslow2 @23:46:46.20 AS.seek 0.506ml @23:46:49.31 Intake.select :close @23:46:49.47 SSV.dial :exhaust @23:46:50.25 Exhaust.select :open @23:46:50.37 SS.reconfigure SSexhale @23:46:50.42 SS.seek 0ml @23:46:52.46 delay 2 seconds @23:46:54.48 ARV.dial :MM1 @23:46:55.04 delay 2 seconds @23:46:57.07 Adding MM1 @23:46:57.14 AS.seek 0.524ml @23:46:59.30 Exhaust.select :close @23:46:59.42 SSV.dial CBV @23:46:59.46 CBV.dial SSV @23:47:00.22 Allowing 130s for 25.0ml of remaining 700.0ml @23:47:00.23 Intake.select :open @23:47:02.42 Intake @33.4psia, vacuum from 20.0 to 24.0psi @23:47:02.44 SS.reconfigure SSinhale.with maxSpeed: 39 @23:47:02.49 SS.seek 25ml @23:47:15.55 delay 25 seconds @23:47:30.49 Vacuum too high -- waiting up to 40s @23:47:32.15 Intake @33.3psia, vacuum from 20.0 to 24.0psi @23:47:32.17 SS.reconfigure SSinhale.with maxSpeed: 35 @23:47:32.22 SS.seek 25ml @23:47:40.58 ARV.dial :PP2 @23:47:40.96 Adding PP2 @23:47:40.98 delay 2 seconds @23:47:43.07 AS.seek 0.53ml @23:47:49.24 delay 10 seconds @23:47:51.86 Stroke completed -- waiting for pressure to equalize ... @23:47:56.39 Intake.select :close @23:47:56.55 SSV.dial :exhaust @23:47:57.31 Exhaust.select :open @23:47:57.43 SS.reconfigure SSexhale @23:47:57.48 SS.seek 0ml @23:47:59.27 ARV.dial :air2 @23:47:59.65 adding ARV air @23:47:59.67 delay 2 seconds @23:48:01.75 AS.seek 0.536ml @23:48:06.37 Exhaust.select :close @23:48:06.49 SSV.dial CBV @23:48:06.51 CBV.dial SSV @23:48:07.30 Allowing 130s for 25.0ml of remaining 675.0ml @23:48:07.31 Intake.select :open @23:48:08.16 delay 5 seconds @23:48:09.49 Intake @33.3psia, vacuum from 20.0 to 24.0psi @23:48:09.51 SS.reconfigure SSinhale.with maxSpeed: 35 @23:48:09.56 SS.seek 25ml @23:48:13.18 ARV.dial :carrier @23:48:14.35 delay 7 seconds @23:48:21.44 AS.seek 0.594ml @23:49:00.00 Stroke completed -- waiting for pressure to equalize ... @23:49:05.16 Intake.select :close @23:49:05.33 SSV.dial :exhaust @23:49:06.11 Exhaust.select :open @23:49:06.22 SS.reconfigure SSexhale @23:49:06.28 SS.seek 0ml @23:49:15.23 Exhaust.select :close @23:49:15.35 SSV.dial CBV @23:49:15.37 CBV.dial SSV @23:49:16.15 Allowing 130s for 25.0ml of remaining 650.0ml @23:49:16.17 Intake.select :open @23:49:18.35 Intake @33.4psia, vacuum from 20.0 to 24.0psi @23:49:18.37 SS.reconfigure SSinhale.with maxSpeed: 35 @23:49:18.42 SS.seek 25ml @23:49:20.04 delay 5 seconds @23:49:25.07 ATV.dial :ASV @23:49:25.43 delay 2 seconds @23:49:27.53 AS.seek 0.6ml @23:49:33.96 delay 4 seconds @23:49:37.99 ATV.dial ARV @23:49:38.60 delay 2 seconds @23:49:40.63 ARV.dial :carrier @23:49:41.15 delay 2 seconds @23:49:43.33 AS.seek 0.65ml @23:50:09.09 Stroke completed -- waiting for pressure to equalize ... @23:50:14.24 Intake.select :close @23:50:14.40 SSV.dial :exhaust @23:50:15.17 Exhaust.select :open @23:50:15.29 SS.reconfigure SSexhale @23:50:15.35 SS.seek 0ml @23:50:24.40 Exhaust.select :close @23:50:24.52 SSV.dial CBV @23:50:24.54 CBV.dial SSV @23:50:25.33 Allowing 130s for 25.0ml of remaining 625.0ml @23:50:25.34 Intake.select :open @23:50:27.53 Intake @33.2psia, vacuum from 20.0 to 24.0psi @23:50:27.55 SS.reconfigure SSinhale.with maxSpeed: 35 @23:50:27.60 SS.seek 25ml @23:50:34.05 delay 4 seconds @23:50:38.08 ATV.dial PCR @23:50:38.76 ASV.dial :waste1 @23:50:39.52 ARV.dial :air1 @23:50:39.91 Positioning Slug by 0.324ml @23:50:40.00 AS.seek 0.326ml @23:51:17.72 Stroke completed -- waiting for pressure to equalize ... @23:51:22.90 Intake.select :close @23:51:23.07 SSV.dial :exhaust @23:51:23.84 Exhaust.select :open @23:51:23.98 SS.reconfigure SSexhale @23:51:24.03 SS.seek 0ml @23:51:32.78 Exhaust.select :close @23:51:32.90 SSV.dial CBV @23:51:32.92 CBV.dial SSV @23:51:33.72 Allowing 130s for 25.0ml of remaining 600.0ml @23:51:33.73 Intake.select :open @23:51:35.92 Intake @33.2psia, vacuum from 20.0 to 24.0psi @23:51:35.94 SS.reconfigure SSinhale.with maxSpeed: 35 @23:51:35.98 SS.seek 25ml @23:52:26.72 Stroke completed -- waiting for pressure to equalize ... @23:52:32.52 Intake.select :close @23:52:32.68 SSV.dial :exhaust @23:52:33.44 Exhaust.select :open @23:52:33.56 SS.reconfigure SSexhale @23:52:33.61 SS.seek 0ml @23:52:42.62 Exhaust.select :close @23:52:42.74 SSV.dial CBV @23:52:42.76 CBV.dial SSV @23:52:44.15 Allowing 130s for 25.0ml of remaining 575.0ml @23:52:44.16 Intake.select :open @23:52:46.35 Intake @33.6psia, vacuum from 20.0 to 24.0psi @23:52:46.37 SS.reconfigure SSinhale.with maxSpeed: 35 @23:52:46.42 SS.seek 25ml @23:53:36.76 Stroke completed -- waiting for pressure to equalize ... @23:53:41.94 Intake.select :close @23:53:42.10 SSV.dial :exhaust @23:53:42.87 Exhaust.select :open @23:53:42.99 SS.reconfigure SSexhale @23:53:43.04 SS.seek 0ml @23:53:52.04 Exhaust.select :close @23:53:52.16 SSV.dial CBV @23:53:52.18 CBV.dial SSV @23:53:52.95 Allowing 130s for 25.0ml of remaining 550.0ml @23:53:52.97 Intake.select :open @23:53:55.15 Intake @33.1psia, vacuum from 20.0 to 24.0psi @23:53:55.17 SS.reconfigure SSinhale.with maxSpeed: 35 @23:53:55.22 SS.seek 25ml @23:54:45.68 Stroke completed -- waiting for pressure to equalize ... @23:54:52.09 Intake.select :close @23:54:52.25 SSV.dial :exhaust @23:54:53.03 Exhaust.select :open @23:54:53.14 SS.reconfigure SSexhale @23:54:53.19 SS.seek 0ml @23:55:02.28 Exhaust.select :close @23:55:02.40 SSV.dial CBV @23:55:02.42 CBV.dial SSV @23:55:03.22 Allowing 130s for 25.0ml of remaining 525.0ml @23:55:03.24 Intake.select :open @23:55:05.42 Intake @33.3psia, vacuum from 20.0 to 24.0psi @23:55:05.44 SS.reconfigure SSinhale.with maxSpeed: 35 @23:55:05.48 SS.seek 25ml @23:55:26.27 BACfans.email "Started mfb in tube 2 at 20:49:33CEST11-Sep-19\n{1 => 9, 2 => 13}\nCan@20:45:23, 16.4C, 79% humidity, 14.6psia, 14.611V, 0.682A, 0.676A avg, 9.97W",Subject: "pcrBAC sampling at most 2000ml, wcr at most 2000ml" @23:55:26.30 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @23:55:26.31 Retry #10 of 10 after 10:00 @23:55:46.27 Vacuum too high -- waiting up to 40s @23:55:47.89 Intake @32.7psia, vacuum from 20.0 to 24.0psi @23:55:47.91 SS.reconfigure SSinhale.with maxSpeed: 32 @23:55:47.96 SS.seek 25ml @23:55:58.18 Stroke completed -- waiting for pressure to equalize ... @23:56:03.33 Intake.select :close @23:56:03.49 SSV.dial :exhaust @23:56:04.28 Exhaust.select :open @23:56:04.40 SS.reconfigure SSexhale @23:56:04.45 SS.seek 0ml @23:56:06.01 delay 5 seconds @23:56:11.04 ATV.dial ARV @23:56:11.76 ASV.dial :PCRlock @23:56:12.13 delay 5 seconds @23:56:13.59 Exhaust.select :close @23:56:13.70 SSV.dial CBV @23:56:13.73 CBV.dial SSV @23:56:14.51 Allowing 130s for 25.0ml of remaining 500.0ml @23:56:14.53 Intake.select :open @23:56:16.71 Intake @33.6psia, vacuum from 20.0 to 24.0psi @23:56:16.73 SS.reconfigure SSinhale.with maxSpeed: 32 @23:56:16.78 SS.seek 25ml @23:56:17.16 Running Pp Profile60C350 thermocycling @23:56:17.18 PCR.program Profile60C350 @23:56:17.32 Profile60C350 should complete in < 1:48:05 @23:56:17.33 PCR.run 2:20:06.25 @23:57:11.73 Stroke completed -- waiting for pressure to equalize ... @23:57:17.53 Intake.select :close @23:57:17.69 SSV.dial :exhaust @23:57:18.47 Exhaust.select :open @23:57:18.59 SS.reconfigure SSexhale @23:57:18.64 SS.seek 0ml @23:57:27.39 Exhaust.select :close @23:57:27.51 SSV.dial CBV @23:57:27.53 CBV.dial SSV @23:57:28.34 Allowing 130s for 25.0ml of remaining 475.0ml @23:57:28.36 Intake.select :open @23:57:30.54 Intake @33.3psia, vacuum from 20.0 to 24.0psi @23:57:30.56 SS.reconfigure SSinhale.with maxSpeed: 32 @23:57:30.61 SS.seek 25ml @23:58:25.90 Stroke completed -- waiting for pressure to equalize ... @23:58:31.75 Intake.select :close @23:58:31.91 SSV.dial :exhaust @23:58:32.67 Exhaust.select :open @23:58:32.78 SS.reconfigure SSexhale @23:58:32.84 SS.seek 0ml @23:58:41.78 Exhaust.select :close @23:58:41.90 SSV.dial CBV @23:58:41.92 CBV.dial SSV @23:58:42.72 Allowing 130s for 25.0ml of remaining 450.0ml @23:58:42.73 Intake.select :open @23:58:44.91 Intake @33.5psia, vacuum from 20.0 to 24.0psi @23:58:44.93 SS.reconfigure SSinhale.with maxSpeed: 32 @23:58:44.98 SS.seek 25ml @23:59:40.51 Stroke completed -- waiting for pressure to equalize ... @23:59:46.96 Intake.select :close @23:59:47.12 SSV.dial :exhaust @23:59:47.90 Exhaust.select :open @23:59:48.02 SS.reconfigure SSexhale @23:59:48.07 SS.seek 0ml @23:59:57.01 Exhaust.select :close @23:59:57.13 SSV.dial CBV @23:59:57.15 CBV.dial SSV @23:59:57.96 Allowing 130s for 25.0ml of remaining 425.0ml @23:59:57.99 Intake.select :open @00:00:00.20CEST12-Sep-19 Intake @33.9psia, vacuum from 20.0 to 24.0psi @00:00:00.22 SS.reconfigure SSinhale.with maxSpeed: 32 @00:00:00.27 SS.seek 25ml @00:00:40.30 Vacuum too high -- waiting up to 40s @00:00:41.92 Intake @33.6psia, vacuum from 20.0 to 24.0psi @00:00:41.94 SS.reconfigure SSinhale.with maxSpeed: 29 @00:00:41.99 SS.seek 25ml @00:00:58.45 Stroke completed -- waiting for pressure to equalize ... @00:01:04.24 Intake.select :close @00:01:04.41 SSV.dial :exhaust @00:01:05.17 Exhaust.select :open @00:01:05.29 SS.reconfigure SSexhale @00:01:05.35 SS.seek 0ml @00:01:14.31 Exhaust.select :close @00:01:14.43 SSV.dial CBV @00:01:14.45 CBV.dial SSV @00:01:15.25 Allowing 130s for 25.0ml of remaining 400.0ml @00:01:15.26 Intake.select :open @00:01:17.45 Intake @34.5psia, vacuum from 20.0 to 24.0psi @00:01:17.47 SS.reconfigure SSinhale.with maxSpeed: 29 @00:01:17.52 SS.seek 25ml @00:02:18.04 Stroke completed -- waiting for pressure to equalize ... @00:02:24.48 Intake.select :close @00:02:24.64 SSV.dial :exhaust @00:02:25.40 Exhaust.select :open @00:02:25.52 SS.reconfigure SSexhale @00:02:25.57 SS.seek 0ml @00:02:34.57 Exhaust.select :close @00:02:34.69 SSV.dial CBV @00:02:34.71 CBV.dial SSV @00:02:35.51 Allowing 130s for 25.0ml of remaining 375.0ml @00:02:35.53 Intake.select :open @00:02:37.71 Intake @33.8psia, vacuum from 20.0 to 24.0psi @00:02:37.73 SS.reconfigure SSinhale.with maxSpeed: 29 @00:02:37.78 SS.seek 25ml @00:03:38.54 Stroke completed -- waiting for pressure to equalize ... @00:03:44.98 Intake.select :close @00:03:45.15 SSV.dial :exhaust @00:03:45.92 Exhaust.select :open @00:03:46.03 SS.reconfigure SSexhale @00:03:46.09 SS.seek 0ml @00:03:55.12 Exhaust.select :close @00:03:55.24 SSV.dial CBV @00:03:55.26 CBV.dial SSV @00:03:56.06 Allowing 130s for 25.0ml of remaining 350.0ml @00:03:56.07 Intake.select :open @00:03:58.26 Intake @33.6psia, vacuum from 20.0 to 24.0psi @00:03:58.28 SS.reconfigure SSinhale.with maxSpeed: 29 @00:03:58.33 SS.seek 25ml @00:04:58.87 Stroke completed -- waiting for pressure to equalize ... @00:05:05.98 Intake.select :close @00:05:06.77 SSV.dial :exhaust @00:05:07.54 Exhaust.select :open @00:05:07.66 SS.reconfigure SSexhale @00:05:07.71 SS.seek 0ml @00:05:16.71 Exhaust.select :close @00:05:16.83 SSV.dial CBV @00:05:16.85 CBV.dial SSV @00:05:17.64 Allowing 130s for 25.0ml of remaining 325.0ml @00:05:17.66 Intake.select :open @00:05:19.84 Intake @33.8psia, vacuum from 20.0 to 24.0psi @00:05:19.86 SS.reconfigure SSinhale.with maxSpeed: 29 @00:05:19.91 SS.seek 25ml @00:05:26.34 BACfans.email "Started mfb in tube 2 at 20:49:33CEST11-Sep-19\n{1 => 9, 2 => 13}\nCan@20:45:23, 16.4C, 79% humidity, 14.6psia, 14.611V, 0.682A, 0.676A avg, 9.97W",Subject: "pcrBAC sampling at most 2000ml, wcr at most 2000ml" @00:05:26.37 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @00:06:20.51 Stroke completed -- waiting for pressure to equalize ... @00:06:27.61 Intake.select :close @00:06:27.77 SSV.dial :exhaust @00:06:28.54 Exhaust.select :open @00:06:28.66 SS.reconfigure SSexhale @00:06:28.71 SS.seek 0ml @00:06:37.62 Exhaust.select :close @00:06:37.74 SSV.dial CBV @00:06:37.76 CBV.dial SSV @00:06:38.57 Allowing 130s for 25.0ml of remaining 299.9ml @00:06:38.59 Intake.select :open @00:06:40.77 Intake @34.1psia, vacuum from 20.0 to 24.0psi @00:06:40.79 SS.reconfigure SSinhale.with maxSpeed: 29 @00:06:40.84 SS.seek 25ml @00:07:21.79 Vacuum too high -- waiting up to 40s @00:07:23.68 Intake @33.8psia, vacuum from 20.0 to 24.0psi @00:07:23.70 SS.reconfigure SSinhale.with maxSpeed: 26 @00:07:23.74 SS.seek 25ml @00:07:46.01 Stroke completed -- waiting for pressure to equalize ... @00:07:52.46 Intake.select :close @00:07:53.25 SSV.dial :exhaust @00:07:54.01 Exhaust.select :open @00:07:54.13 SS.reconfigure SSexhale @00:07:54.18 SS.seek 0ml @00:08:03.12 Exhaust.select :close @00:08:03.23 SSV.dial CBV @00:08:03.26 CBV.dial SSV @00:08:04.04 Allowing 130s for 25.0ml of remaining 274.9ml @00:08:04.06 Intake.select :open @00:08:06.23 Intake @33.5psia, vacuum from 20.0 to 24.0psi @00:08:06.25 SS.reconfigure SSinhale.with maxSpeed: 26 @00:08:06.30 SS.seek 25ml @00:09:13.89 Stroke completed -- waiting for pressure to equalize ... @00:09:20.95 Intake.select :close @00:09:21.11 SSV.dial :exhaust @00:09:21.89 Exhaust.select :open @00:09:22.01 SS.reconfigure SSexhale @00:09:22.06 SS.seek 0ml @00:09:31.10 Exhaust.select :close @00:09:31.22 SSV.dial CBV @00:09:31.24 CBV.dial SSV @00:09:32.03 Allowing 130s for 25.0ml of remaining 249.9ml @00:09:32.05 Intake.select :open @00:09:34.23 Intake @34.0psia, vacuum from 20.0 to 24.0psi @00:09:34.25 SS.reconfigure SSinhale.with maxSpeed: 26 @00:09:34.29 SS.seek 25ml @00:10:41.51 Stroke completed -- waiting for pressure to equalize ... @00:10:48.61 Intake.select :close @00:10:48.77 SSV.dial :exhaust @00:10:49.53 Exhaust.select :open @00:10:49.65 SS.reconfigure SSexhale @00:10:49.70 SS.seek 0ml @00:10:58.79 Exhaust.select :close @00:10:58.91 SSV.dial CBV @00:10:58.95 CBV.dial SSV @00:10:59.70 Allowing 130s for 25.0ml of remaining 224.9ml @00:10:59.71 Intake.select :open @00:11:01.96 Intake @34.0psia, vacuum from 20.0 to 24.0psi @00:11:02.00 SS.reconfigure SSinhale.with maxSpeed: 26 @00:11:02.10 SS.seek 25ml @00:12:10.06 Stroke completed -- waiting for pressure to equalize ... @00:12:17.17 Intake.select :close @00:12:17.33 SSV.dial :exhaust @00:12:18.10 Exhaust.select :open @00:12:18.22 SS.reconfigure SSexhale @00:12:18.27 SS.seek 0ml @00:12:27.28 Exhaust.select :close @00:12:27.40 SSV.dial CBV @00:12:27.42 CBV.dial SSV @00:12:28.23 Allowing 130s for 25.0ml of remaining 199.9ml @00:12:28.25 Intake.select :open @00:12:30.43 Intake @33.4psia, vacuum from 20.0 to 24.0psi @00:12:30.45 SS.reconfigure SSinhale.with maxSpeed: 26 @00:12:30.50 SS.seek 25ml @00:13:38.01 Stroke completed -- waiting for pressure to equalize ... @00:13:46.37 Intake.select :close @00:13:46.53 SSV.dial :exhaust @00:13:47.29 Exhaust.select :open @00:13:47.41 SS.reconfigure SSexhale @00:13:47.46 SS.seek 0ml @00:13:56.15 Exhaust.select :close @00:13:56.27 SSV.dial CBV @00:13:56.29 CBV.dial SSV @00:13:57.10 Allowing 130s for 25.0ml of remaining 174.9ml @00:13:57.12 Intake.select :open @00:13:59.29 Intake @33.8psia, vacuum from 20.0 to 24.0psi @00:13:59.31 SS.reconfigure SSinhale.with maxSpeed: 26 @00:13:59.36 SS.seek 25ml @00:14:58.85 Vacuum too high -- waiting up to 40s @00:15:01.14 Intake @33.5psia, vacuum from 20.0 to 24.0psi @00:15:01.16 SS.reconfigure SSinhale.with maxSpeed: 23 @00:15:01.21 SS.seek 25ml @00:15:11.13 Stroke completed -- waiting for pressure to equalize ... @00:15:18.19 Intake.select :close @00:15:18.36 SSV.dial :exhaust @00:15:19.13 Exhaust.select :open @00:15:19.25 SS.reconfigure SSexhale @00:15:19.30 SS.seek 0ml @00:15:28.38 Exhaust.select :close @00:15:28.50 SSV.dial CBV @00:15:28.52 CBV.dial SSV @00:15:29.31 Allowing 130s for 25.0ml of remaining 149.9ml @00:15:29.33 Intake.select :open @00:15:31.51 Intake @34.2psia, vacuum from 20.0 to 24.0psi @00:15:31.53 SS.reconfigure SSinhale.with maxSpeed: 23 @00:15:31.58 SS.seek 25ml @00:16:47.54 Stroke completed -- waiting for pressure to equalize ... @00:16:55.22 Intake.select :close @00:16:55.39 SSV.dial :exhaust @00:16:56.15 Exhaust.select :open @00:16:56.26 SS.reconfigure SSexhale @00:16:56.33 SS.seek 0ml @00:17:05.12 Exhaust.select :close @00:17:05.24 SSV.dial CBV @00:17:05.26 CBV.dial SSV @00:17:06.06 Allowing 130s for 25.0ml of remaining 124.9ml @00:17:06.07 Intake.select :open @00:17:08.26 Intake @33.2psia, vacuum from 20.0 to 24.0psi @00:17:08.28 SS.reconfigure SSinhale.with maxSpeed: 23 @00:17:08.32 SS.seek 25ml @00:18:24.80 Stroke completed -- waiting for pressure to equalize ... @00:18:33.12 Intake.select :close @00:18:33.28 SSV.dial :exhaust @00:18:34.06 Exhaust.select :open @00:18:34.18 SS.reconfigure SSexhale @00:18:34.23 SS.seek 0ml @00:18:43.26 Exhaust.select :close @00:18:43.38 SSV.dial CBV @00:18:43.40 CBV.dial SSV @00:18:44.20 Allowing 130s for 25.0ml of remaining 99.9ml @00:18:44.21 Intake.select :open @00:18:46.40 Intake @33.5psia, vacuum from 20.0 to 24.0psi @00:18:46.42 SS.reconfigure SSinhale.with maxSpeed: 23 @00:18:46.47 SS.seek 25ml @00:20:02.68 Stroke completed -- waiting for pressure to equalize ... @00:20:12.28 Intake.select :close @00:20:12.44 SSV.dial :exhaust @00:20:13.22 Exhaust.select :open @00:20:13.34 SS.reconfigure SSexhale @00:20:13.39 SS.seek 0ml @00:20:22.37 Exhaust.select :close @00:20:22.48 SSV.dial CBV @00:20:22.51 CBV.dial SSV @00:20:23.30 Allowing 130s for 25.0ml of remaining 74.9ml @00:20:23.32 Intake.select :open @00:20:25.50 Intake @33.3psia, vacuum from 20.0 to 24.0psi @00:20:25.52 SS.reconfigure SSinhale.with maxSpeed: 23 @00:20:25.56 SS.seek 25ml @00:21:42.04 Stroke completed -- waiting for pressure to equalize ... @00:21:51.62 Intake.select :close @00:21:51.78 SSV.dial :exhaust @00:21:52.56 Exhaust.select :open @00:21:52.68 SS.reconfigure SSexhale @00:21:52.73 SS.seek 0ml @00:22:01.59 Exhaust.select :close @00:22:01.71 SSV.dial CBV @00:22:01.73 CBV.dial SSV @00:22:02.54 Allowing 130s for 25.0ml of remaining 49.9ml @00:22:02.56 Intake.select :open @00:22:04.74 Intake @33.8psia, vacuum from 20.0 to 24.0psi @00:22:04.76 SS.reconfigure SSinhale.with maxSpeed: 23 @00:22:04.81 SS.seek 25ml @00:23:12.64 Vacuum too high -- waiting up to 40s @00:23:15.30 Intake @34.1psia, vacuum from 20.0 to 24.0psi @00:23:15.32 SS.reconfigure SSinhale.with maxSpeed: 21 @00:23:15.36 SS.seek 25ml @00:23:24.97 Stroke completed -- waiting for pressure to equalize ... @00:23:33.94 Intake.select :close @00:23:34.10 SSV.dial :exhaust @00:23:34.87 Exhaust.select :open @00:23:34.99 SS.reconfigure SSexhale @00:23:35.04 SS.seek 0ml @00:23:43.84 Exhaust.select :close @00:23:43.96 SSV.dial CBV @00:23:43.98 CBV.dial SSV @00:23:45.40 Allowing 130s for 24.9ml of remaining 24.9ml @00:23:45.41 Intake.select :open @00:23:47.60 Intake @33.9psia, vacuum from 20.0 to 24.0psi @00:23:47.62 SS.reconfigure SSinhale.with maxSpeed: 21 @00:23:47.67 SS.seek 24.94ml @00:25:11.16 Stroke completed -- waiting for pressure to equalize ... @00:25:21.37 Intake.select :close @00:25:21.54 SSV.dial :exhaust @00:25:22.32 Exhaust.select :open @00:25:22.44 SS.reconfigure SSexhale @00:25:22.49 SS.seek 0ml @00:25:31.44 Exhaust.select :close @00:25:31.55 Sampled 2000.0ml @00:25:31.57 Drying puck before fixing for 4:00 @00:25:31.58 CBV.dial :waste2b @00:25:32.34 delay 1:00 @00:26:32.37 CSV.dial :air @00:26:32.94 CS.reconfigure CSfast @00:26:32.99 CS.seek 10ml @00:26:55.62 Pumping wcr puck from tube 2 up to 55psi... @00:26:55.63 CBV.dial SSV @00:26:56.96 CBV.dial between CSV and waste2b @00:26:57.96 CSV.dial between air and CTV @00:26:58.29 CS.seek 6.683ml @00:27:06.51 CTV.dial CSV,avoiding: :air @00:27:06.53 CSV.dial CTV @00:27:07.38 CS.reconfigure CSpressurize @00:27:07.43 CS.seek 0ml @00:27:24.27 CBV.dial :waste2b @00:27:24.62 CS.reconfigure CSconfig @00:27:24.67 CS.seek :empty @00:27:26.40 delay 1:00 @00:28:26.43 CTV.dial :air,avoiding: :intake @00:28:28.34 Fix #1 of 2 (with rnal) for 2:00 @00:28:28.35 CSV.dial CSR @00:28:28.86 Pulling 2ml of CSR.rnal @00:28:28.87 CSR.select :rnal @00:28:28.99 CS.seek 2ml @00:28:35.19 delay 5 seconds @00:28:40.22 CSR.select :air @00:28:40.35 CS.reconfigure CSfast @00:28:40.40 CS.seek 10ml @00:28:58.50 Pumping wcr puck from tube 2 up to 55psi... @00:28:58.52 CBV.dial SSV @00:28:59.85 CBV.dial between CSV and waste2b @00:29:00.85 CSV.dial between air and CTV @00:29:01.48 CS.seek 6.661ml @00:29:09.82 CTV.dial CSV,avoiding: :air @00:29:09.84 CSV.dial CTV @00:29:11.23 CS.reconfigure CSpressurize @00:29:11.28 CS.seek 0ml @00:29:27.61 delay 2:00 @00:31:27.64 CBV.dial :waste2b @00:31:27.98 CS.reconfigure CSconfig @00:31:28.04 CS.seek :empty @00:31:30.22 delay 1:00 @00:32:30.24 CTV.dial :air,avoiding: :intake @00:32:31.53 Fix #2 of 2 (with rnal) for 2:00 @00:32:31.55 CSV.dial CSR @00:32:32.06 Pulling 2ml of CSR.rnal @00:32:32.08 CSR.select :rnal @00:32:32.20 CS.seek 2ml @00:32:38.38 delay 5 seconds @00:32:43.41 CSR.select :air @00:32:43.53 CS.reconfigure CSfast @00:32:43.59 CS.seek 10ml @00:33:01.72 Pumping wcr puck from tube 2 up to 55psi... @00:33:01.74 CBV.dial SSV @00:33:03.06 CBV.dial between CSV and waste2b @00:33:04.06 CSV.dial between air and CTV @00:33:04.68 CS.seek 6.661ml @00:33:13.01 CTV.dial CSV,avoiding: :air @00:33:13.05 CSV.dial CTV @00:33:14.39 CS.reconfigure CSpressurize @00:33:14.45 CS.seek 0ml @00:33:30.62 delay 2:00 @00:35:30.65 CBV.dial :waste2b @00:35:31.00 CS.reconfigure CSconfig @00:35:31.06 CS.seek :empty @00:35:33.38 delay 1:00 @00:36:33.41 CTV.dial :air,avoiding: :intake @00:36:34.79 Storing wcr puck in Collection Clamp to tube 1 @00:36:34.99 SC.reconfigure SCtight @00:36:35.04 SC.seek 1 @00:36:37.17 SC.reconfigure SCloose @00:36:37.41 Elbow.reconfigure TightElbow @00:36:37.46 Elbow.seek 1 @00:36:39.34 Forearm.reconfigure TightForearm @00:36:39.40 Forearm.seek 1 + 700 counts @00:36:40.42 SE.reconfigure SEpick @00:36:40.50 SE.seek beamBreak @00:36:42.53 SE.configure SEconfig @00:36:42.71 Elbow.reconfigure LimpElbow @00:36:42.76 Forearm.reconfigure LooseForearm @00:36:42.81 Forearm.seek 1 @00:36:43.10 Elbow.hold @00:36:43.18 Elbow.reconfigure TightElbow @00:36:43.29 SE.seek 10.07 Pucks below Top Plate @00:36:43.87 Forearm.force -20 @00:36:43.92 Forearm.seek 1 + 345 counts @00:36:44.27 Forearm.reconfigure TightForearm @00:36:44.32 Forearm.seek 1 + 700 counts @00:36:44.66 SE.seek 11.07 Pucks below Top Plate @00:36:45.38 Forearm.reconfigure LooseForearm @00:36:45.43 Forearm.seek :retract @00:36:46.38 Elbow.seek CC @00:36:48.03 Elbow.reconfigure LooseElbow @00:36:48.14 Forearm.seek CC @00:36:49.20 Hand.close @00:36:49.87 CC.seek :open @00:36:54.38 Forearm.seek :retract @00:36:55.40 Elbow.reconfigure TightElbow @00:36:56.06 Elbow.seek 1 @00:36:57.98 Elbow.reconfigure LimpElbow @00:36:58.04 Forearm.seek 1 @00:36:59.07 Elbow.hold @00:36:59.15 Elbow.reconfigure TightElbow @00:36:59.20 Hand.open @00:37:00.01 Forearm.force -20 @00:37:00.05 Forearm.seek 1 + 345 counts @00:37:00.38 Forearm.reconfigure TightForearm @00:37:00.44 Forearm.seek 1 + 700 counts @00:37:00.79 SE.seek :down @00:37:02.01 Forearm.reconfigure LooseForearm @00:37:02.06 Forearm.seek :retract @00:37:02.60 SE.coast @00:37:02.96 SC.coast @00:37:03.01 Starting finalPurge @00:37:03.02 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @00:37:03.10 Hand.open @00:37:03.51 Elbow.seek FlushPuck::Garage @00:37:05.47 Elbow.reconfigure LooseElbow @00:37:05.58 Forearm.seek :garage @00:37:06.59 Hand.close @00:37:07.38 Forearm.seek :retract @00:37:08.41 Elbow.reconfigure TightElbow @00:37:08.46 Elbow.seek CC @00:37:11.42 Elbow.reconfigure LooseElbow @00:37:11.52 Forearm.reconfigure TightForearm @00:37:11.57 Forearm.seek CC @00:37:12.91 CC.seek :closed @00:37:17.19 Collection Clamp onSpring/offSpring for 6/32 samples, on/off torque 1.76 @00:37:17.27 Hand.open @00:37:17.94 Forearm.reconfigure LooseForearm @00:37:17.99 Forearm.seek :retract @00:37:18.99 Flushing the Collection Valve::Manifold @00:37:19.13 CSV.dial CSR @00:37:19.65 Pulling 5ml of CSR.flush CSR.select :flush @00:37:19.78 CS.seek 5ml @00:37:33.60 delay 5 seconds @00:37:38.63 CSR.select :air @00:37:38.81 CS.reconfigure CSfast @00:37:38.86 CS.seek 7.501ml @00:37:46.00 CSV.dial :waste @00:37:47.27 CS.reconfigure CSconfig @00:37:47.32 CS.seek 0ml @00:38:07.50 Flushing lower manifold's waste1 port @00:38:07.64 CSV.dial :flush @00:38:08.12 CS.reconfigure CSfast @00:38:08.16 CS.seek 5ml @00:38:19.97 delay 5 seconds @00:38:24.99 CSV.dial :air @00:38:25.52 CS.seek 7ml @00:38:30.88 CSV.dial CTV @00:38:30.91
CTV.dial CSV @00:38:32.30 CBV.dial :waste1 @00:38:33.08 CS.seek 0ml @00:38:49.19 Flushing lower manifold's waste2b port @00:38:49.33 CSV.dial :flush @00:38:50.22 CS.seek 5ml @00:39:01.94 delay 5 seconds @00:39:06.96 CSV.dial :air @00:39:07.48 CS.seek 7ml @00:39:12.97 CSV.dial CTV @00:39:13.01
CTV.dial CSV @00:39:13.46 CBV.dial :waste2b @00:39:14.25 CS.seek 0ml @00:39:30.16 Flushing upper manifold @00:39:30.30 CSV.dial :flush @00:39:31.06 CS.seek 5ml @00:39:42.88 delay 5 seconds @00:39:47.90 CSV.dial :air @00:39:48.44 CS.seek 7ml @00:39:53.79 CSV.dial CBV @00:39:53.82
CBV.dial CSV @00:39:54.93 CTV.dial :air @00:39:56.25 CS.seek 0ml @00:40:12.16 delay 7 seconds @00:40:19.19 CS.seek 3ml @00:40:26.73 CSV.dial :waste @00:40:27.20 CS.seek 0ml @00:40:35.01 CSV.dial :flush @00:40:35.47 CS.seek 3ml @00:40:42.94 delay 5 seconds @00:40:47.96 CSV.dial :air @00:40:48.48 CS.seek 5ml @00:40:53.88 CSV.dial :air @00:40:53.90 CS.seek 0ml @00:41:05.73 delay 7 seconds @00:41:12.76 CS.seek 3ml @00:41:20.32 CSV.dial :waste @00:41:21.06 CS.seek 0ml @00:41:28.57 Disinfecting external Intake with mfbkill @00:41:28.71 CSV.dial CSR @00:41:30.06 Pulling 8ml of CSR.mfbkill CSR.select :mfbkill @00:41:30.19 CS.seek 8ml @00:41:49.10 delay 5 seconds @00:41:54.13 CSR.select :air @00:41:54.26 CS.seek 10ml @00:41:59.66 CSV.dial CBV @00:41:59.71
CBV.dial CSV @00:42:00.76 CTV.dial :intake @00:42:01.52 Intake.select :open @00:42:01.64 CS.seek 2ml @00:42:20.76 Intake.select :close @00:42:20.87 CTV.dial :air @00:42:21.62 CS.reconfigure CSconfig @00:42:21.67 CS.seek 0ml @00:42:27.82 Flushing lower manifold's waste1 port @00:42:27.83 CSV.dial CSR @00:42:28.91 Pulling 4ml of CSR.mfbkill @00:42:28.92 CSR.select :mfbkill @00:42:29.04 CS.seek 4ml @00:42:40.45 delay 5 seconds @00:42:45.48 CSR.select :air @00:42:45.61 CS.reconfigure CSfast @00:42:45.65 CS.seek 10ml @00:42:59.60 CSV.dial CTV @00:42:59.64
CTV.dial CSV @00:43:01.02 CBV.dial :waste1 @00:43:01.49 CS.seek 0ml @00:43:23.95 Flushing lower manifold's waste2b port @00:43:23.96 CSV.dial CSR @00:43:24.50 Pulling 4ml of CSR.mfbkill @00:43:24.51 CSR.select :mfbkill @00:43:24.63 CS.reconfigure CSconfig @00:43:24.68 CS.seek 4ml @00:43:35.95 delay 5 seconds @00:43:40.98 CSR.select :air @00:43:41.11 CS.reconfigure CSfast @00:43:41.15 CS.seek 10ml @00:43:55.10 CSV.dial CTV @00:43:55.14
CTV.dial CSV @00:43:55.60 CBV.dial :waste2b @00:43:56.38 CS.seek 0ml @00:44:19.07 Killing upper manifold @00:44:19.08 CSV.dial CSR @00:44:19.60 Pulling 4ml of CSR.mfbkill @00:44:19.61 CSR.select :mfbkill @00:44:19.74 CS.reconfigure CSconfig @00:44:19.79 CS.seek 4ml @00:44:31.07 delay 5 seconds @00:44:36.10 CSR.select :air @00:44:36.22 CS.reconfigure CSfast @00:44:36.26 CS.seek 10ml @00:44:50.20 CSV.dial CBV @00:44:50.23
CBV.dial CSV @00:44:51.29 CTV.dial :air @00:44:52.61 CS.seek 0ml @00:45:15.07 delay 7 seconds @00:45:22.10 CS.seek 3ml @00:45:29.76 CSV.dial :waste @00:45:30.21 CS.seek 0ml @00:45:37.76 Flushing upper manifold @00:45:37.90 CSV.dial :flush @00:45:38.35 CS.seek 5ml @00:45:50.04 delay 5 seconds @00:45:55.07 CSV.dial :air @00:45:56.20 CS.seek 7.001ml @00:46:01.54 CSV.dial CBV @00:46:01.57
CBV.dial CSV @00:46:02.60 CTV.dial :air @00:46:02.62 CS.seek 0ml @00:46:18.57 delay 7 seconds @00:46:25.62 CS.seek 3ml @00:46:33.26 CSV.dial :waste @00:46:33.72 CS.seek 0ml @00:46:41.28 CSV.dial :flush @00:46:41.75 CS.seek 3ml @00:46:49.29 delay 5 seconds @00:46:54.32 CSV.dial :air @00:46:54.86 CS.seek 5ml @00:47:00.20 CSV.dial :air @00:47:00.22 CS.seek 0ml @00:47:11.82 delay 7 seconds @00:47:18.85 CS.seek 3ml @00:47:26.29 CSV.dial :waste @00:47:27.04 CS.seek 0ml @00:47:34.51 Flushing lower manifold's waste1 port @00:47:34.65 CSV.dial :flush @00:47:35.13 CS.seek 5ml @00:47:46.82 delay 5 seconds @00:47:51.84 CSV.dial :air @00:47:52.37 CS.seek 7ml @00:47:57.79 CSV.dial CTV @00:47:57.82
CTV.dial CSV @00:47:59.20 CBV.dial :waste1 @00:47:59.69 CS.seek 0ml @00:48:15.64 Flushing lower manifold's waste2b port @00:48:15.78 CSV.dial :flush @00:48:16.54 CS.seek 5ml @00:48:28.32 delay 5 seconds @00:48:33.34 CSV.dial :air @00:48:33.88 CS.seek 7ml @00:48:39.32 CSV.dial CTV @00:48:39.35
CTV.dial CSV @00:48:39.81 CBV.dial :waste2b @00:48:40.58 CS.seek 0ml @00:48:56.51 Disinfecting external Intake with kill @00:48:56.65 CSV.dial CSR @00:48:57.18 Pulling 8ml of CSR.kill CSR.select :kill @00:48:57.31 CS.seek 8ml @00:49:15.61 delay 5 seconds @00:49:20.64 CSR.select :air @00:49:20.77 CS.seek 10ml @00:49:26.12 CSV.dial CBV @00:49:26.15
CBV.dial CSV @00:49:27.23 CTV.dial :intake @00:49:27.98 Intake.select :open @00:49:28.10 CS.seek 2ml @00:49:47.20 Intake.select :close @00:49:47.31 CTV.dial :air @00:49:48.08 CS.reconfigure CSconfig @00:49:48.13 CS.seek 0ml @00:49:54.83 CSV.dial CSR @00:49:55.91 Pulling 5ml of CSR.flush @00:49:55.92 CSR.select :flush @00:49:56.04 CS.seek 5ml @00:50:09.89 delay 5 seconds @00:50:14.92 CSR.select :air @00:50:15.05 CS.seek 10ml @00:50:28.76 CSV.dial CBV @00:50:29.77 CS.seek 0ml @00:50:56.30 Garaging FlushPuck in Collection Clamp @00:50:56.39 Elbow.reconfigure TightElbow @00:50:56.43 Elbow.seek CC @00:50:56.75 Elbow.reconfigure LooseElbow @00:50:56.86 Forearm.seek CC @00:50:57.84 Hand.close @00:50:58.51 CC.seek :open @00:51:02.90 Forearm.seek :retract @00:51:03.93 Elbow.reconfigure TightElbow @00:51:03.98 Elbow.seek :garage @00:51:06.97 Elbow.reconfigure LooseElbow @00:51:07.08 Forearm.reconfigure TightForearm @00:51:07.13 Forearm.seek :garage @00:51:08.35 Hand.open @00:51:09.08 Forearm.reconfigure LooseForearm @00:51:09.13 Forearm.seek :retract @00:51:10.21 Completed [mbaPhase] begun 20:45:23 @00:51:10.22 Preparing to sleep until 20:43 12-september-19 @00:51:10.27 deferred until MFBPCR finish @00:51:10.29
Waiting for MFBPCR til 20:43 12-september-19 @00:51:10.32 Continuing at 20:43 @00:51:10.39 GoodNews.email "Then, in tube 2: wcrPhase2\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:129\n{1 => 12, 2 => 10}\nCan@00:51:10, 17.4C, 79% humidity, 14.6psia, 14.370V, 0.569A, 0.569A avg, 8.18W",Subject: "Waiting until 20:43" @00:51:10.42 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @00:51:10.43 Retry #1 of 10 after 10:00 @01:01:10.46 GoodNews.email "Then, in tube 2: wcrPhase2\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:129\n{1 => 12, 2 => 10}\nCan@00:51:10, 17.4C, 79% humidity, 14.6psia, 14.370V, 0.569A, 0.569A avg, 8.18W",Subject: "Waiting until 20:43" @01:01:10.49 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @01:01:10.51 Retry #2 of 10 after 10:00 @01:11:10.53 GoodNews.email "Then, in tube 2: wcrPhase2\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:129\n{1 => 12, 2 => 10}\nCan@00:51:10, 17.4C, 79% humidity, 14.6psia, 14.370V, 0.569A, 0.569A avg, 8.18W",Subject: "Waiting until 20:43" @01:11:10.56 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @01:11:10.58 Retry #3 of 10 after 10:00 @01:19:20.86 PCR.off @01:19:23.04 PCR for Pp completed -- closed /var/log/esp/Pp19sep1123h1450ml.pcr @01:19:23.12 Cleaning PCR @01:19:23.13 AFV.select :carrierSolution @01:19:23.24 delay 2 seconds @01:19:25.27 AS.reconfigure ASconfig @01:19:25.32 AS.seek 0.5ml @01:19:35.63 delay 1 second @01:19:36.65 AFV.select :holdingCoil @01:19:36.75 ASV.dial :waste1 @01:19:37.13 ATV.dial :air3 @01:19:38.34 AS.reconfigure ASslow1 @01:19:38.39 AS.seek 0.51ml @01:19:40.68 delay 2 seconds @01:19:42.70 ATV.dial :bleach @01:19:43.29 AS.seek 0.81ml @01:20:43.88 delay 3 seconds @01:20:46.91 ATV.dial :air3 @01:20:47.42 delay 3 seconds @01:20:50.51 AS.seek 0.835ml @01:20:55.78 delay 3 seconds @01:20:58.81 ATV.dial :bleach @01:20:59.40 AS.seek 0.82ml @01:21:02.71 delay 3 seconds @01:21:05.74 ARV.dial :waste @01:21:06.33 delay 3 seconds @01:21:09.36 ATV.dial :ARV @01:21:10.20 AS.reconfigure ASconfig @01:21:10.25 AS.seek 0.47ml @01:21:10.60 GoodNews.email "Then, in tube 2: wcrPhase2\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:129\n{1 => 12, 2 => 10}\nCan@00:51:10, 17.4C, 79% humidity, 14.6psia, 14.370V, 0.569A, 0.569A avg, 8.18W",Subject: "Waiting until 20:43" @01:21:10.63 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @01:21:10.65 Retry #4 of 10 after 10:00 @01:21:30.67 AFV.select :carrierSolution @01:21:30.78 delay 2 seconds @01:21:32.82 AS.seek 1ml @01:22:03.58 delay 1 second @01:22:04.60 AFV.select :holdingCoil @01:22:04.70 delay 1 second @01:22:05.73 AS.reconfigure ASfast @01:22:05.78 AS.seek 0ml @01:22:33.13 AFV.select :carrierSolution @01:22:33.24 delay 2 seconds @01:22:35.27 AS.reconfigure ASconfig @01:22:35.32 AS.seek 1ml @01:23:33.13 delay 1 second @01:23:34.15 AFV.select :holdingCoil @01:23:34.25 delay 1 second @01:23:35.28 AS.reconfigure ASfast @01:23:35.33 AS.seek 0ml @01:24:02.69 AFV.select :carrierSolution @01:24:02.80 delay 2 seconds @01:24:04.83 AS.reconfigure ASconfig @01:24:04.88 AS.seek 1ml @01:25:02.72 delay 1 second @01:25:03.75 AFV.select :holdingCoil @01:25:03.85 delay 1 second @01:25:04.88 AS.reconfigure ASfast @01:25:04.93 AS.seek 0ml @01:25:32.27 ARV.dial between carrier and air1 @01:25:32.75 ATV.dial :air3 @01:25:33.96 AS.reconfigure ASslow1 @01:25:34.01 AS.seek 0.01ml @01:25:36.36 delay 2 seconds @01:25:38.38 ATV.dial :bleach @01:25:38.96 AS.seek 0.81ml @01:28:20.10 delay 3 seconds @01:28:23.13 ATV.dial :air3 @01:28:23.64 delay 3 seconds @01:28:26.79 AS.seek 0.835ml @01:28:32.11 delay 3 seconds @01:28:35.14 ATV.dial :bleach @01:28:35.73 AS.seek 0.82ml @01:28:39.05 delay 3 seconds @01:28:42.08 ATV.dial PCR @01:28:42.46 AS.reconfigure ASconfig @01:28:42.51 AS.seek :empty @01:29:29.96 PCR.program :bleachProfile @01:29:30.01 PCR program should complete in < 2:45 @01:29:30.02 PCR.run 10:00 @01:31:10.67 GoodNews.email "Then, in tube 2: wcrPhase2\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:129\n{1 => 12, 2 => 10}\nCan@00:51:10, 17.4C, 79% humidity, 14.6psia, 14.370V, 0.569A, 0.569A avg, 8.18W",Subject: "Waiting until 20:43" @01:31:10.70 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @01:31:10.71 Retry #5 of 10 after 10:00 @01:32:35.35 PCR.off @01:32:35.38 AFV.select :carrierSolution @01:32:35.49 delay 2 seconds @01:32:37.53 AS.seek 1ml @01:33:35.35 delay 1 second @01:33:36.37 AFV.select :holdingCoil @01:33:36.47 delay 1 second @01:33:37.50 AS.reconfigure ASfast @01:33:37.55 AS.seek 0ml @01:34:04.91 delay 3 seconds @01:34:07.94 AFV.select :carrierSolution @01:34:08.04 delay 2 seconds @01:34:10.07 AS.reconfigure ASconfig @01:34:10.12 AS.seek 1ml @01:35:07.86 delay 1 second @01:35:08.88 AFV.select :holdingCoil @01:35:08.98 delay 1 second @01:35:10.01 AS.reconfigure ASfast @01:35:10.06 AS.seek 0ml @01:35:37.38 delay 3 seconds @01:35:40.41 AFV.select :carrierSolution @01:35:40.51 delay 2 seconds @01:35:42.55 AS.reconfigure ASconfig @01:35:42.60 AS.seek 1ml @01:36:40.40 delay 1 second @01:36:41.42 AFV.select :holdingCoil @01:36:41.52 delay 1 second @01:36:42.55 AS.reconfigure ASfast @01:36:42.60 AS.seek 0ml @01:37:09.94 delay 3 seconds @01:37:12.97 AFV.select :carrierSolution @01:37:13.07 delay 2 seconds @01:37:15.10 AS.reconfigure ASconfig @01:37:15.15 AS.seek 1ml @01:38:12.93 delay 1 second @01:38:13.95 AFV.select :holdingCoil @01:38:14.05 delay 1 second @01:38:15.08 AS.reconfigure ASfast @01:38:15.13 AS.seek 0ml @01:38:42.47 delay 3 seconds @01:38:45.50 AFV.select :carrierSolution @01:38:45.60 delay 2 seconds @01:38:47.63 AS.reconfigure ASconfig @01:38:47.68 AS.seek 1ml @01:39:45.46 delay 1 second @01:39:46.48 AFV.select :holdingCoil @01:39:46.58 delay 1 second @01:39:47.61 AS.reconfigure ASnorm @01:39:47.66 AS.seek 0ml @01:40:38.39 Setting up PCR for Mm -- writing /var/log/esp/Mm19sep1201h1450ml.pcr @01:40:38.41 Building PCR Slug from ASV with MM2 mix, PP3 primer @01:40:38.42 Priming with carrier @01:40:38.43 ATV.dial ARV @01:40:39.02 ARV.dial :carrier @01:40:39.37 AS.seek 0.5ml @01:41:04.99 AFV.select :carrierSolution @01:41:05.10 delay 2 seconds @01:41:07.13 AS.reconfigure ASconfig @01:41:07.18 AS.seek 1ml @01:41:10.74 GoodNews.email "Then, in tube 2: wcrPhase2\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:129\n{1 => 12, 2 => 10}\nCan@00:51:10, 17.4C, 79% humidity, 14.6psia, 14.370V, 0.569A, 0.569A avg, 8.18W",Subject: "Waiting until 20:43" @01:41:10.77 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @01:41:10.79 Retry #6 of 10 after 10:00 @01:41:36.21 delay 1 second @01:41:37.24 AFV.select :holdingCoil @01:41:37.34 delay 1 second @01:41:38.37 ATV.dial :waste @01:41:39.91 AS.reconfigure ASnorm @01:41:39.96 AS.seek :empty @01:42:30.69 AFV.select :carrierSolution @01:42:30.80 delay 2 seconds @01:42:32.83 AS.reconfigure ASconfig @01:42:32.88 AS.seek 0.5ml @01:43:01.98 delay 1 second @01:43:03.00 AFV.select :holdingCoil @01:43:03.10 Building Slug @01:43:03.11 ATV.dial :ARV @01:43:04.63 delay 2 seconds @01:43:06.66 ARV.dial :air1 @01:43:07.05 delay 2 seconds @01:43:09.14 AS.reconfigure ASslow2 @01:43:09.19 AS.seek 0.506ml @01:43:15.48 delay 2 seconds @01:43:17.51 ARV.dial :MM2 @01:43:18.96 delay 2 seconds @01:43:20.99 Adding MM2 @01:43:21.06 AS.seek 0.524ml @01:43:39.62 delay 25 seconds @01:44:04.65 ARV.dial :PP3 @01:44:05.03 Adding PP3 @01:44:05.04 delay 2 seconds @01:44:07.12 AS.seek 0.53ml @01:44:13.48 delay 10 seconds @01:44:23.51 ARV.dial :air2 @01:44:23.90 adding ARV air @01:44:23.92 delay 2 seconds @01:44:26.00 AS.seek 0.536ml @01:44:32.34 delay 5 seconds @01:44:37.37 ARV.dial :carrier @01:44:38.54 delay 7 seconds @01:44:45.63 AS.seek 0.594ml @01:45:44.29 delay 5 seconds @01:45:49.32 ATV.dial :ASV @01:45:49.69 delay 2 seconds @01:45:51.79 AS.seek 0.6ml @01:45:58.16 delay 4 seconds @01:46:02.19 ATV.dial ARV @01:46:02.80 delay 2 seconds @01:46:04.83 ARV.dial :carrier @01:46:04.84 delay 2 seconds @01:46:07.52 AS.seek 0.65ml @01:46:58.05 delay 4 seconds @01:47:02.08 ATV.dial PCR @01:47:02.65 ASV.dial :waste1 @01:47:02.66 ARV.dial :air1 @01:47:03.06 Positioning Slug by 0.324ml @01:47:03.15 AS.seek 0.326ml @01:51:10.81 GoodNews.email "Then, in tube 2: wcrPhase2\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:129\n{1 => 12, 2 => 10}\nCan@00:51:10, 17.4C, 79% humidity, 14.6psia, 14.370V, 0.569A, 0.569A avg, 8.18W",Subject: "Waiting until 20:43" @01:51:10.84 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @01:51:10.86 Retry #7 of 10 after 10:00 @01:52:29.20 delay 5 seconds @01:52:34.23 ATV.dial ARV @01:52:34.95 ASV.dial :PCRlock @01:52:35.34 delay 5 seconds @01:52:40.37 Running Mm Profile60C350 thermocycling @01:52:40.38 PCR.program Profile60C350 @01:52:40.52 Profile60C350 should complete in < 1:48:05 @01:52:40.53 PCR.run 2:20:06.25 @02:01:10.88 GoodNews.email "Then, in tube 2: wcrPhase2\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:129\n{1 => 12, 2 => 10}\nCan@00:51:10, 17.4C, 79% humidity, 14.6psia, 14.370V, 0.569A, 0.569A avg, 8.18W",Subject: "Waiting until 20:43" @02:01:10.91 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @02:01:10.93 Retry #8 of 10 after 10:00 @02:11:10.95 GoodNews.email "Then, in tube 2: wcrPhase2\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:129\n{1 => 12, 2 => 10}\nCan@00:51:10, 17.4C, 79% humidity, 14.6psia, 14.370V, 0.569A, 0.569A avg, 8.18W",Subject: "Waiting until 20:43" @02:11:10.98 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @02:11:11.00 Retry #9 of 10 after 10:00 @02:21:11.02 GoodNews.email "Then, in tube 2: wcrPhase2\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:129\n{1 => 12, 2 => 10}\nCan@00:51:10, 17.4C, 79% humidity, 14.6psia, 14.370V, 0.569A, 0.569A avg, 8.18W",Subject: "Waiting until 20:43" @02:21:11.05 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @02:21:11.06 Retry #10 of 10 after 10:00 @02:31:11.09 GoodNews.email "Then, in tube 2: wcrPhase2\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:129\n{1 => 12, 2 => 10}\nCan@00:51:10, 17.4C, 79% humidity, 14.6psia, 14.370V, 0.569A, 0.569A avg, 8.18W",Subject: "Waiting until 20:43" @02:31:11.12 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @03:15:38.17 PCR.off @03:15:40.24 PCR for Mm completed -- closed /var/log/esp/Mm19sep1201h1450ml.pcr @03:15:40.31 Cleaning PCR @03:15:40.32 AFV.select :carrierSolution @03:15:40.43 delay 2 seconds @03:15:42.46 AS.reconfigure ASconfig @03:15:42.51 AS.seek 0.5ml @03:15:52.80 delay 1 second @03:15:53.82 AFV.select :holdingCoil @03:15:53.92 ASV.dial :waste1 @03:15:54.30 ATV.dial :air3 @03:15:55.50 AS.reconfigure ASslow1 @03:15:55.55 AS.seek 0.51ml @03:15:57.84 delay 2 seconds @03:15:59.86 ATV.dial :bleach @03:16:00.45 AS.seek 0.81ml @03:17:01.05 delay 3 seconds @03:17:04.08 ATV.dial :air3 @03:17:04.60 delay 3 seconds @03:17:07.69 AS.seek 0.835ml @03:17:13.00 delay 3 seconds @03:17:16.03 ATV.dial :bleach @03:17:16.62 AS.seek 0.82ml @03:17:19.94 delay 3 seconds @03:17:22.97 ARV.dial :waste @03:17:23.55 delay 3 seconds @03:17:26.58 ATV.dial :ARV @03:17:27.42 AS.reconfigure ASconfig @03:17:27.47 AS.seek 0.47ml @03:17:47.87 AFV.select :carrierSolution @03:17:47.98 delay 2 seconds @03:17:50.02 AS.seek 1ml @03:18:20.76 delay 1 second @03:18:21.78 AFV.select :holdingCoil @03:18:21.88 delay 1 second @03:18:22.91 AS.reconfigure ASfast @03:18:22.96 AS.seek 0ml @03:18:50.30 AFV.select :carrierSolution @03:18:50.41 delay 2 seconds @03:18:52.44 AS.reconfigure ASconfig @03:18:52.49 AS.seek 1ml @03:19:50.24 delay 1 second @03:19:51.26 AFV.select :holdingCoil @03:19:51.36 delay 1 second @03:19:52.39 AS.reconfigure ASfast @03:19:52.44 AS.seek 0ml @03:20:19.80 AFV.select :carrierSolution @03:20:19.91 delay 2 seconds @03:20:21.94 AS.reconfigure ASconfig @03:20:21.99 AS.seek 1ml @03:21:19.76 delay 1 second @03:21:20.78 AFV.select :holdingCoil @03:21:20.88 delay 1 second @03:21:21.91 AS.reconfigure ASfast @03:21:21.96 AS.seek 0ml @03:21:49.32 ARV.dial between carrier and air1 @03:21:49.80 ATV.dial :air3 @03:21:50.98 AS.reconfigure ASslow1 @03:21:51.03 AS.seek 0.01ml @03:21:53.34 delay 2 seconds @03:21:55.36 ATV.dial :bleach @03:21:56.53 AS.seek 0.81ml @03:24:37.66 delay 3 seconds @03:24:40.69 ATV.dial :air3 @03:24:41.21 delay 3 seconds @03:24:44.30 AS.seek 0.835ml @03:24:49.59 delay 3 seconds @03:24:52.62 ATV.dial :bleach @03:24:53.22 AS.seek 0.82ml @03:24:56.51 delay 3 seconds @03:24:59.54 ATV.dial PCR @03:24:59.91 AS.reconfigure ASconfig @03:24:59.96 AS.seek :empty @03:25:47.43 PCR.program :bleachProfile @03:25:47.48 PCR program should complete in < 2:45 @03:25:47.49 PCR.run 10:00 @03:28:52.34 PCR.off @03:28:52.37 AFV.select :carrierSolution @03:28:52.48 delay 2 seconds @03:28:54.52 AS.seek 1ml @03:29:52.37 delay 1 second @03:29:53.39 AFV.select :holdingCoil @03:29:53.49 delay 1 second @03:29:54.52 AS.reconfigure ASfast @03:29:54.57 AS.seek 0ml @03:30:22.02 delay 3 seconds @03:30:25.05 AFV.select :carrierSolution @03:30:25.16 delay 2 seconds @03:30:27.19 AS.reconfigure ASconfig @03:30:27.25 AS.seek 1ml @03:31:25.02 delay 1 second @03:31:26.04 AFV.select :holdingCoil @03:31:26.14 delay 1 second @03:31:27.17 AS.reconfigure ASfast @03:31:27.22 AS.seek 0ml @03:31:54.54 delay 3 seconds @03:31:57.57 AFV.select :carrierSolution @03:31:57.67 delay 2 seconds @03:31:59.70 AS.reconfigure ASconfig @03:31:59.75 AS.seek 1ml @03:32:57.49 delay 1 second @03:32:58.51 AFV.select :holdingCoil @03:32:58.61 delay 1 second @03:32:59.64 AS.reconfigure ASfast @03:32:59.69 AS.seek 0ml @03:33:27.03 delay 3 seconds @03:33:30.06 AFV.select :carrierSolution @03:33:30.16 delay 2 seconds @03:33:32.19 AS.reconfigure ASconfig @03:33:32.25 AS.seek 1ml @03:34:30.06 delay 1 second @03:34:31.08 AFV.select :holdingCoil @03:34:31.18 delay 1 second @03:34:32.21 AS.reconfigure ASfast @03:34:32.26 AS.seek 0ml @03:34:59.60 delay 3 seconds @03:35:02.63 AFV.select :carrierSolution @03:35:02.73 delay 2 seconds @03:35:04.76 AS.reconfigure ASconfig @03:35:04.82 AS.seek 1ml @03:36:02.60 delay 1 second @03:36:03.62 AFV.select :holdingCoil @03:36:03.72 delay 1 second @03:36:04.75 AS.reconfigure ASnorm @03:36:04.80 AS.seek 0ml @03:36:55.49 Setting up PCR for Ss -- writing /var/log/esp/Ss19sep1203h1450ml.pcr @03:36:55.51 Building PCR Slug from ASV with MM2 mix, PP4 primer @03:36:55.53 Priming with carrier ATV.dial ARV @03:36:56.12 ARV.dial :carrier @03:36:56.47 AS.seek 0.5ml @03:37:22.01 AFV.select :carrierSolution @03:37:22.12 delay 2 seconds @03:37:24.15 AS.reconfigure ASconfig @03:37:24.20 AS.seek 1ml @03:37:53.28 delay 1 second @03:37:54.30 AFV.select :holdingCoil @03:37:54.40 delay 1 second @03:37:55.43 ATV.dial :waste @03:37:57.06 AS.reconfigure ASnorm @03:37:57.11 AS.seek :empty @03:38:47.84 AFV.select :carrierSolution @03:38:47.95 delay 2 seconds @03:38:49.98 AS.reconfigure ASconfig @03:38:50.03 AS.seek 0.5ml @03:39:19.14 delay 1 second @03:39:20.16 AFV.select :holdingCoil @03:39:20.26 Building Slug @03:39:20.27 ATV.dial :ARV @03:39:21.78 delay 2 seconds @03:39:23.81 ARV.dial :air1 @03:39:24.19 delay 2 seconds @03:39:26.27 AS.reconfigure ASslow2 @03:39:26.33 AS.seek 0.506ml @03:39:32.64 delay 2 seconds @03:39:34.66 ARV.dial :MM2 @03:39:36.01 delay 2 seconds @03:39:38.04 Adding MM2 @03:39:38.12 AS.seek 0.524ml @03:39:56.51 delay 25 seconds @03:40:22.12 ARV.dial :PP4 @03:40:22.53 Adding PP4 @03:40:22.55 delay 2 seconds @03:40:24.63 AS.seek 0.53ml @03:40:31.00 delay 10 seconds @03:40:41.03 ARV.dial :air3 @03:40:41.40 adding ARV air @03:40:41.42 delay 2 seconds @03:40:43.50 AS.seek 0.536ml @03:40:49.79 delay 5 seconds @03:40:54.82 ARV.dial :carrier @03:40:55.98 delay 7 seconds @03:41:03.07 AS.seek 0.594ml @03:42:01.76 delay 5 seconds @03:42:06.79 ATV.dial :ASV @03:42:07.17 delay 2 seconds @03:42:09.26 AS.seek 0.6ml @03:42:15.51 delay 4 seconds @03:42:19.54 ATV.dial ARV @03:42:20.04 delay 2 seconds @03:42:22.07 ARV.dial :carrier @03:42:22.08 delay 2 seconds @03:42:24.17 AS.seek 0.65ml @03:43:14.78 delay 4 seconds @03:43:18.81 ATV.dial PCR @03:43:19.37 ASV.dial :waste1 @03:43:19.38 ARV.dial :air1 @03:43:19.76 Positioning Slug by 0.324ml @03:43:19.86 AS.seek 0.326ml @03:48:45.75 delay 5 seconds @03:48:50.78 ATV.dial ARV @03:48:51.50 ASV.dial :PCRlock @03:48:51.89 delay 5 seconds @03:48:56.92 Running Ss Profile60C350 thermocycling @03:48:56.93 PCR.program Profile60C350 @03:48:57.07 Profile60C350 should complete in < 1:48:05 @03:48:57.08 PCR.run 2:20:06.25 @05:11:53.03 PCR.off @05:11:55.09 PCR for Ss completed -- closed /var/log/esp/Ss19sep1203h1450ml.pcr @05:11:55.16 Cleaning PCR @05:11:55.18 AFV.select :carrierSolution @05:11:55.29 delay 2 seconds @05:11:57.32 AS.reconfigure ASconfig @05:11:57.37 AS.seek 0.5ml @05:12:07.68 delay 1 second @05:12:08.70 AFV.select :holdingCoil @05:12:08.80 ASV.dial :waste1 @05:12:09.18 ATV.dial :air3 @05:12:10.38 AS.reconfigure ASslow1 @05:12:10.43 AS.seek 0.51ml @05:12:12.72 delay 2 seconds @05:12:14.74 ATV.dial :bleach @05:12:15.37 AS.seek 0.81ml @05:13:16.00 delay 3 seconds @05:13:19.03 ATV.dial :air3 @05:13:19.56 delay 3 seconds @05:13:22.65 AS.seek 0.835ml @05:13:27.94 delay 3 seconds @05:13:30.97 ATV.dial :bleach @05:13:31.57 AS.seek 0.82ml @05:13:34.85 delay 3 seconds @05:13:37.88 ARV.dial :waste @05:13:38.45 delay 3 seconds @05:13:41.48 ATV.dial :ARV @05:13:42.32 AS.reconfigure ASconfig @05:13:42.37 AS.seek 0.47ml @05:14:02.78 AFV.select :carrierSolution @05:14:02.89 delay 2 seconds @05:14:04.93 AS.seek 1ml @05:14:35.67 delay 1 second @05:14:36.70 AFV.select :holdingCoil @05:14:36.80 delay 1 second @05:14:37.83 AS.reconfigure ASfast @05:14:37.88 AS.seek 0ml @05:15:05.25 AFV.select :carrierSolution @05:15:05.36 delay 2 seconds @05:15:07.39 AS.reconfigure ASconfig @05:15:07.44 AS.seek 1ml @05:16:05.25 delay 1 second @05:16:06.27 AFV.select :holdingCoil @05:16:06.37 delay 1 second @05:16:07.40 AS.reconfigure ASfast @05:16:07.45 AS.seek 0ml @05:16:34.79 AFV.select :carrierSolution @05:16:34.90 delay 2 seconds @05:16:36.93 AS.reconfigure ASconfig @05:16:37.63 AS.seek 1ml @05:17:35.42 delay 1 second @05:17:36.44 AFV.select :holdingCoil @05:17:36.54 delay 1 second @05:17:37.57 AS.reconfigure ASfast @05:17:37.62 AS.seek 0ml @05:18:04.97 ARV.dial between carrier and air1 @05:18:05.57 ATV.dial :air3 @05:18:06.77 AS.reconfigure ASslow1 @05:18:06.82 AS.seek 0.01ml @05:18:09.17 delay 2 seconds @05:18:11.19 ATV.dial :bleach @05:18:11.79 AS.seek 0.81ml @05:20:52.92 delay 3 seconds @05:20:55.95 ATV.dial :air3 @05:20:56.47 delay 3 seconds @05:20:59.56 AS.seek 0.835ml @05:21:04.87 delay 3 seconds @05:21:07.90 ATV.dial :bleach @05:21:08.49 AS.seek 0.82ml @05:21:11.78 delay 3 seconds @05:21:14.81 ATV.dial PCR @05:21:15.18 AS.reconfigure ASconfig @05:21:15.23 AS.seek :empty @05:22:02.70 PCR.program :bleachProfile @05:22:02.75 PCR program should complete in < 2:45 @05:22:02.76 PCR.run 10:00 @05:25:07.60 PCR.off @05:25:07.63 AFV.select :carrierSolution @05:25:07.74 delay 2 seconds @05:25:09.78 AS.seek 1ml @05:26:07.61 delay 1 second @05:26:08.63 AFV.select :holdingCoil @05:26:08.73 delay 1 second @05:26:09.76 AS.reconfigure ASfast @05:26:09.81 AS.seek 0ml @05:26:37.12 delay 3 seconds @05:26:40.15 AFV.select :carrierSolution @05:26:40.25 delay 2 seconds @05:26:42.28 AS.reconfigure ASconfig @05:26:42.34 AS.seek 1ml @05:27:40.12 delay 1 second @05:27:41.14 AFV.select :holdingCoil @05:27:41.24 delay 1 second @05:27:42.27 AS.reconfigure ASfast @05:27:42.32 AS.seek 0ml @05:28:09.67 delay 3 seconds @05:28:12.70 AFV.select :carrierSolution @05:28:12.80 delay 2 seconds @05:28:14.83 AS.reconfigure ASconfig @05:28:14.89 AS.seek 1ml @05:29:12.70 delay 1 second @05:29:13.72 AFV.select :holdingCoil @05:29:13.82 delay 1 second @05:29:14.85 AS.reconfigure ASfast @05:29:14.91 AS.seek 0ml @05:29:42.28 delay 3 seconds @05:29:45.31 AFV.select :carrierSolution @05:29:45.41 delay 2 seconds @05:29:47.44 AS.reconfigure ASconfig @05:29:47.50 AS.seek 1ml @05:30:45.28 delay 1 second @05:30:46.30 AFV.select :holdingCoil @05:30:46.40 delay 1 second @05:30:47.43 AS.reconfigure ASfast @05:30:47.48 AS.seek 0ml @05:31:14.84 delay 3 seconds @05:31:17.87 AFV.select :carrierSolution @05:31:17.97 delay 2 seconds @05:31:20.00 AS.reconfigure ASconfig @05:31:20.06 AS.seek 1ml @05:32:17.87 delay 1 second @05:32:18.89 AFV.select :holdingCoil @05:32:18.99 delay 1 second @05:32:20.02 AS.reconfigure ASnorm @05:32:20.07 AS.seek 0ml @05:33:10.80 Setting up PCR for IPc -- writing /var/log/esp/IPc19sep1205h1450ml.pcr @05:33:10.81 Building PCR Slug from ASV with MM1 mix, PP1 primer @05:33:10.83 Priming with carrier @05:33:10.84 ATV.dial ARV @05:33:11.42 ARV.dial :carrier @05:33:11.76 AS.seek 0.5ml @05:33:37.32 AFV.select :carrierSolution @05:33:37.44 delay 2 seconds @05:33:39.47 AS.reconfigure ASconfig @05:33:39.52 AS.seek 1ml @05:34:08.58 delay 1 second @05:34:09.60 AFV.select :holdingCoil @05:34:09.70 delay 1 second @05:34:10.73 ATV.dial :waste @05:34:12.25 AS.reconfigure ASnorm @05:34:12.30 AS.seek :empty @05:35:03.05 AFV.select :carrierSolution @05:35:03.17 delay 2 seconds @05:35:05.20 AS.reconfigure ASconfig @05:35:05.25 AS.seek 0.5ml @05:35:34.33 delay 1 second @05:35:35.35 AFV.select :holdingCoil @05:35:36.07 Building Slug @05:35:36.08 ATV.dial :ARV @05:35:37.61 delay 2 seconds @05:35:39.64 ARV.dial :air1 @05:35:40.00 delay 2 seconds @05:35:42.09 AS.reconfigure ASslow2 @05:35:42.14 AS.seek 0.506ml @05:35:48.42 delay 2 seconds @05:35:50.45 ARV.dial :MM1 @05:35:51.02 delay 2 seconds @05:35:53.05 Adding MM1 @05:35:53.12 AS.seek 0.524ml @05:36:11.66 delay 25 seconds @05:36:36.69 ARV.dial :PP1 @05:36:37.08 Adding PP1 @05:36:37.10 delay 2 seconds @05:36:39.18 AS.seek 0.53ml @05:36:45.45 delay 10 seconds @05:36:55.48 ARV.dial :air1 @05:36:55.84 adding ARV air @05:36:55.86 delay 2 seconds @05:36:57.94 AS.seek 0.536ml @05:37:04.58 delay 5 seconds @05:37:09.61 ARV.dial :carrier @05:37:09.98 delay 7 seconds @05:37:17.07 AS.seek 0.594ml @05:38:15.62 delay 5 seconds @05:38:20.65 ATV.dial :ASV @05:38:21.03 delay 2 seconds @05:38:23.12 AS.seek 0.6ml @05:38:29.53 delay 4 seconds @05:38:33.56 ATV.dial ARV @05:38:34.06 delay 2 seconds @05:38:36.09 ARV.dial :carrier @05:38:36.10 delay 2 seconds @05:38:38.19 AS.seek 0.65ml @05:39:28.75 delay 4 seconds @05:39:32.78 ATV.dial PCR @05:39:33.34 ASV.dial :waste1 @05:39:33.35 ARV.dial :air1 @05:39:33.73 Positioning Slug by 0.324ml @05:39:33.83 AS.seek 0.326ml @05:44:59.72 delay 5 seconds @05:45:04.75 ATV.dial ARV @05:45:05.47 ASV.dial :PCRlock @05:45:05.86 delay 5 seconds @05:45:10.89 Running IPc ProfileIPc thermocycling @05:45:10.90 PCR.program ProfileIPc @05:45:11.06 ProfileIPc should complete in < 1:50:49 @05:45:11.07 PCR.run 2:23:31.25 @07:09:35.25 PCR.off @07:09:37.85 PCR for IPc completed -- closed /var/log/esp/IPc19sep1205h1450ml.pcr @07:09:37.87 Full MFB Decontamination @07:09:37.88 Decon extraction column and decon AIV @07:09:37.90 SPE.hold 2:46:40,55C @07:09:37.94 ASV.dial :SPEdelivery @07:09:38.55 delay 20 seconds @07:09:58.59 AFV.select :carrierSolution @07:09:58.69 delay 2 seconds @07:10:00.72 AS.reconfigure ASconfig @07:10:00.77 AS.seek 0.5ml @07:10:11.66 delay 1 second @07:10:12.68 AFV.select :holdingCoil @07:10:12.81 ATV.dial :air1 @07:10:14.49 AS.reconfigure ASslow1 @07:10:14.54 AS.seek 0.52ml @07:10:18.83 ATV.dial :ASV @07:10:20.38 ASV.dial :SPErecovery @07:10:20.99 AS.seek 0.62ml @07:10:41.38 ATV.dial :waste @07:10:42.94 AS.reconfigure ASnorm @07:10:42.99 AS.seek :empty @07:11:14.61 AFV.select :holdingCoil @07:11:14.62 ATV.dial :waste @07:11:14.64 AS.reconfigure ASconfig @07:11:14.69 AS.seek 0ml @07:11:15.00 AFV.select :carrierSolution @07:11:15.11 delay 2 seconds @07:11:17.15 AS.seek 0.5ml @07:11:46.17 delay 1 second @07:11:47.19 AFV.select :holdingCoil @07:11:47.29 ATV.dial :air3 @07:11:48.09 AS.reconfigure ASslow1 @07:11:48.14 AS.seek 0.52ml @07:11:52.50 ATV.dial :air3 @07:11:52.59 AS.seek 0.53ml @07:11:54.88 delay 2 seconds @07:11:56.90 ATV.dial :bleach @07:11:57.35 AS.seek 0.71ml @07:12:33.80 delay 3 seconds @07:12:36.83 ATV.dial :air3 @07:12:37.34 delay 3 seconds @07:12:40.43 AS.seek 0.735ml @07:12:45.72 delay 3 seconds @07:12:48.75 ATV.dial :bleach @07:12:49.34 AS.seek 0.72ml @07:12:52.64 delay 3 seconds @07:12:55.67 ATV.dial ASV @07:12:56.64 ASV.dial :SPErecovery @07:12:56.73 AS.seek 0.365ml @07:14:08.38 ATV.dial :waste @07:14:09.97 AS.reconfigure ASnorm @07:14:10.02 AS.seek :empty @07:14:28.78 ATV.dial :air3 @07:14:29.45 AS.reconfigure ASslow1 @07:14:29.50 AS.seek 0.009ml @07:14:31.89 delay 2 seconds @07:14:33.91 ATV.dial :bleach @07:14:34.37 AS.seek 0.579ml @07:16:29.24 delay 3 seconds @07:16:32.27 ATV.dial :air3 @07:16:32.80 delay 3 seconds @07:16:35.89 AS.seek 0.604ml @07:16:41.24 delay 3 seconds @07:16:44.27 ATV.dial :bleach @07:16:44.87 AS.seek 0.589ml @07:16:48.17 delay 3 seconds @07:16:51.20 ATV.dial :ASV @07:16:52.16 ASV.dial :wasteR @07:16:52.54 AS.seek 0ml @07:18:51.28 delay 1:00 @07:19:51.31 ATV.dial ASV @07:19:51.32 AFV.select :carrierSolution @07:19:51.42 delay 2 seconds @07:19:53.45 AS.reconfigure ASconfig @07:19:53.50 AS.seek 1ml @07:20:51.30 delay 1 second @07:20:52.32 AFV.select :holdingCoil @07:20:52.42 delay 1 second @07:20:53.45 ASV.dial :wasteR @07:20:53.48 AS.reconfigure ASslow1 @07:20:53.53 AS.seek 0ml @07:24:14.96 delay 10 seconds @07:24:24.99 ATV.dial :air3 @07:24:26.39 AS.seek 0.01ml @07:24:28.75 delay 2 seconds @07:24:30.77 ATV.dial :bleach @07:24:31.41 AS.seek 0.58ml @07:26:26.41 delay 3 seconds @07:26:29.44 ATV.dial :air3 @07:26:29.96 delay 3 seconds @07:26:33.05 AS.seek 0.605ml @07:26:38.37 delay 3 seconds @07:26:41.40 ATV.dial :bleach @07:26:42.62 AS.seek 0.59ml @07:26:45.92 delay 3 seconds @07:26:48.95 ATV.dial ASV @07:26:49.91 ASV.dial :SPEdelivery @07:26:50.28 AS.seek 0ml @07:28:49.18 delay 1:00 @07:29:49.21 AFV.select :carrierSolution @07:29:49.31 delay 2 seconds @07:29:51.34 AS.reconfigure ASconfig @07:29:51.39 AS.seek 1ml @07:30:49.16 delay 1 second @07:30:50.19 AFV.select :holdingCoil @07:30:50.29 delay 1 second @07:30:51.32 ATV.dial ASV @07:30:51.33 ASV.dial :SPEdelivery @07:30:51.35 AS.reconfigure ASslow1 @07:30:51.39 AS.seek 0ml @07:34:12.77 delay 10 seconds @07:34:22.80 ATV.dial :air2 @07:34:23.59 AS.reconfigure ASslow0 @07:34:23.64 AS.seek 0.8ml @07:35:44.43 delay 3 seconds @07:35:47.46 ATV.dial :ASV @07:35:48.47 ASV.dial :SPEdelivery @07:35:48.49 AS.reconfigure ASslow1 @07:35:48.54 AS.seek 0.03ml @07:38:23.66 delay 20 seconds @07:38:43.69 ATV.dial :waste,via: :air3 @07:38:45.27 AS.reconfigure ASconfig @07:38:45.32 AS.seek :empty @07:38:47.39 SPE.coast @07:38:47.49 Removing Bleach from Mixing Coil @07:38:47.50 AFV.select :carrierSolution @07:38:47.60 delay 2 seconds @07:38:49.64 AS.seek 0.5ml @07:39:18.74 delay 1 second @07:39:19.76 AFV.select :holdingCoil @07:39:19.86 ATV.dial :ASV @07:39:21.44 ASV.dial :SPErecovery @07:39:22.07 AS.reconfigure ASslow1 @07:39:22.12 AS.seek 0.73ml @07:40:08.65 ASV.dial :wasteD @07:40:09.30 AS.reconfigure ASnorm @07:40:09.35 AS.seek 0ml @07:40:46.47 AFV.select :carrierSolution @07:40:46.58 delay 2 seconds @07:40:48.61 AS.reconfigure ASconfig @07:40:48.66 AS.seek 0.5ml @07:41:17.72 delay 1 second @07:41:18.74 AFV.select :holdingCoil @07:41:18.84 ATV.dial ASV @07:41:18.85 ASV.dial :SPErecovery @07:41:19.47 AS.reconfigure ASslow0 @07:41:19.52 AS.seek 0.3ml @07:41:39.99 ATV.dial :air2 @07:41:41.00 AS.reconfigure ASslow1 @07:41:41.05 AS.seek 0.32ml @07:41:45.37 ATV.dial ASV @07:41:46.37 AS.reconfigure ASslow0 @07:41:46.42 AS.seek 0.57ml @07:42:11.80 ASV.dial :wasteD @07:42:12.44 AS.reconfigure ASnorm @07:42:12.50 AS.seek 0ml @07:42:41.55 AFV.select :carrierSolution @07:42:41.66 delay 2 seconds @07:42:43.69 AS.reconfigure ASconfig @07:42:43.74 AS.seek 0.5ml @07:43:12.80 delay 1 second @07:43:13.82 AFV.select :holdingCoil @07:43:13.92 ATV.dial ASV @07:43:13.93 ASV.dial :SPErecovery @07:43:14.54 AS.reconfigure ASslow0 @07:43:14.59 AS.seek 0.3ml @07:43:35.01 ATV.dial :air2 @07:43:36.01 AS.reconfigure ASslow1 @07:43:36.06 AS.seek 0.32ml @07:43:40.42 ATV.dial ASV @07:43:41.40 AS.reconfigure ASslow0 @07:43:41.45 AS.seek 0.57ml @07:44:06.85 ASV.dial :wasteD @07:44:07.51 AS.reconfigure ASnorm @07:44:07.57 AS.seek 0ml @07:44:36.63 ATV.dial :air2 @07:44:37.54 AS.reconfigure ASslow0 @07:44:37.60 AS.seek 0.3ml @07:45:08.15 ATV.dial :ASV @07:45:09.07 ASV.dial :wasteD @07:45:09.09 AS.reconfigure ASconfig @07:45:09.14 AS.seek 0.05ml @07:45:23.83 ATV.dial :waste @07:45:25.56 AS.seek 0ml @07:45:29.38 ASV.dial :SPEdelivery @07:45:30.29 deconLysateINT @07:45:30.30 ATV.dial :air3 @07:45:30.97 AS.reconfigure ASslow1 @07:45:31.02 AS.seek 0.01ml @07:45:33.37 delay 2 seconds @07:45:35.39 ATV.dial :bleach @07:45:35.84 AS.seek 0.96ml @07:48:47.13 delay 3 seconds @07:48:50.16 ATV.dial :air3 @07:48:50.68 delay 3 seconds @07:48:53.77 AS.seek 0.985ml @07:48:59.06 delay 3 seconds @07:49:02.09 ATV.dial :bleach @07:49:02.76 AS.seek 0.97ml @07:49:06.05 delay 3 seconds @07:49:09.08 ATV.dial AIV @07:49:10.24 AIV.dial :coilToMFB @07:49:10.26 AWV.select :waste @07:49:10.27 AFV.select :carrierSolution @07:49:10.38 delay 2 seconds @07:49:12.41 AS.reconfigure ASconfig @07:49:12.46 AS.seek 1ml @07:49:14.49 delay 1 second @07:49:15.51 AFV.select :holdingCoil @07:49:15.61 delay 1 second @07:49:16.64 AS.reconfigure ASslow0 @07:49:16.69 AS.seek :empty @07:50:57.65 AFV.select :carrierSolution @07:50:57.76 delay 2 seconds @07:50:59.79 AS.reconfigure ASconfig @07:50:59.84 AS.seek 1ml @07:51:57.65 delay 1 second @07:51:58.67 AFV.select :holdingCoil @07:51:58.77 delay 1 second @07:51:59.80 AS.reconfigure ASslow0 @07:51:59.85 AS.seek :empty @07:53:40.84 AFV.select :carrierSolution @07:53:40.95 delay 2 seconds @07:53:42.98 AS.reconfigure ASconfig @07:53:43.03 AS.seek 1ml @07:54:40.84 delay 1 second @07:54:41.86 AFV.select :holdingCoil @07:54:41.96 delay 1 second @07:54:42.99 AS.reconfigure ASslow0 @07:54:43.04 AS.seek :empty @07:56:24.01 ATV.dial :air2 @07:56:24.66 AS.reconfigure ASslow1 @07:56:24.71 AS.seek 0.009ml @07:56:27.01 AS.reconfigure ASslow0 @07:56:27.06 AS.seek 1ml @07:58:06.98 ATV.dial AIV @07:58:07.71 AS.seek 0.02ml @07:59:46.59 ATV.dial :waste @07:59:48.13 AS.seek 0ml @07:59:50.43 AWV.select :air @07:59:50.60 Cleaning PCR @07:59:50.61 AFV.select :carrierSolution @07:59:50.71 delay 2 seconds @07:59:52.74 AS.reconfigure ASconfig @07:59:52.79 AS.seek 0.5ml @08:00:21.84 delay 1 second @08:00:22.86 AFV.select :holdingCoil @08:00:22.96 ASV.dial :waste1 @08:00:23.32 ATV.dial :air3 @08:00:23.97 AS.reconfigure ASslow1 @08:00:24.02 AS.seek 0.51ml @08:00:26.29 delay 2 seconds @08:00:28.31 ATV.dial :bleach @08:00:28.77 AS.seek 0.81ml @08:01:29.37 delay 3 seconds @08:01:32.40 ATV.dial :air3 @08:01:32.91 delay 3 seconds @08:01:36.00 AS.seek 0.835ml @08:01:41.31 delay 3 seconds @08:01:44.34 ATV.dial :bleach @08:01:44.94 AS.seek 0.82ml @08:01:48.26 delay 3 seconds @08:01:51.29 ARV.dial :waste @08:01:51.85 delay 3 seconds @08:01:54.88 ATV.dial :ARV @08:01:55.72 AS.reconfigure ASconfig @08:01:55.77 AS.seek 0.47ml @08:02:16.19 AFV.select :carrierSolution @08:02:16.31 delay 2 seconds @08:02:18.35 AS.seek 1ml @08:02:49.09 delay 1 second @08:02:50.11 AFV.select :holdingCoil @08:02:50.21 delay 1 second @08:02:51.24 AS.reconfigure ASfast @08:02:51.29 AS.seek 0ml @08:03:18.63 AFV.select :carrierSolution @08:03:18.74 delay 2 seconds @08:03:20.77 AS.reconfigure ASconfig @08:03:20.82 AS.seek 1ml @08:04:18.60 delay 1 second @08:04:20.21 AFV.select :holdingCoil @08:04:20.38 delay 1 second @08:04:21.42 AS.reconfigure ASfast @08:04:21.47 AS.seek 0ml @08:04:48.81 AFV.select :carrierSolution @08:04:48.92 delay 2 seconds @08:04:50.95 AS.reconfigure ASconfig @08:04:51.00 AS.seek 1ml @08:05:48.77 delay 1 second @08:05:49.79 AFV.select :holdingCoil @08:05:49.89 delay 1 second @08:05:50.92 AS.reconfigure ASfast @08:05:50.97 AS.seek 0ml @08:06:18.31 ARV.dial between carrier and air1 @08:06:18.77 ATV.dial :air3 @08:06:19.97 AS.reconfigure ASslow1 @08:06:20.02 AS.seek 0.01ml @08:06:22.38 delay 2 seconds @08:06:24.40 ATV.dial :bleach @08:06:24.99 AS.seek 0.81ml @08:09:06.15 delay 3 seconds @08:09:09.18 ATV.dial :air3 @08:09:09.70 delay 3 seconds @08:09:12.79 AS.seek 0.835ml @08:09:18.10 delay 3 seconds @08:09:21.13 ATV.dial :bleach @08:09:21.72 AS.seek 0.82ml @08:09:25.01 delay 3 seconds @08:09:28.04 ATV.dial PCR @08:09:28.45 AS.reconfigure ASconfig @08:09:28.50 AS.seek :empty @08:10:15.96 PCR.program :bleachProfile @08:10:16.01 PCR program should complete in < 2:45 @08:10:16.02 PCR.run 10:00 @08:13:33.00 PCR.off @08:13:33.03 AFV.select :carrierSolution @08:13:33.14 delay 2 seconds @08:13:35.18 AS.seek 1ml @08:14:33.01 delay 1 second @08:14:34.03 AFV.select :holdingCoil @08:14:34.13 delay 1 second @08:14:35.16 AS.reconfigure ASfast @08:14:35.21 AS.seek 0ml @08:15:02.57 delay 3 seconds @08:15:05.60 AFV.select :carrierSolution @08:15:05.70 delay 2 seconds @08:15:07.73 AS.reconfigure ASconfig @08:15:07.79 AS.seek 1ml @08:16:05.59 delay 1 second @08:16:06.62 AFV.select :holdingCoil @08:16:06.72 delay 1 second @08:16:07.75 AS.reconfigure ASfast @08:16:07.80 AS.seek 0ml @08:16:35.15 delay 3 seconds @08:16:38.18 AFV.select :carrierSolution @08:16:38.28 delay 2 seconds @08:16:40.31 AS.reconfigure ASconfig @08:16:40.37 AS.seek 1ml @08:17:38.15 delay 1 second @08:17:39.17 AFV.select :holdingCoil @08:17:39.27 delay 1 second @08:17:40.30 AS.reconfigure ASfast @08:17:40.35 AS.seek 0ml @08:18:07.70 delay 3 seconds @08:18:10.73 AFV.select :carrierSolution @08:18:10.83 delay 2 seconds @08:18:12.86 AS.reconfigure ASconfig @08:18:12.92 AS.seek 1ml @08:19:10.67 delay 1 second @08:19:11.69 AFV.select :holdingCoil @08:19:11.79 delay 1 second @08:19:12.82 AS.reconfigure ASfast @08:19:12.87 AS.seek 0ml @08:19:40.18 delay 3 seconds @08:19:43.21 AFV.select :carrierSolution @08:19:43.31 delay 2 seconds @08:19:45.34 AS.reconfigure ASconfig @08:19:45.39 AS.seek 1ml @08:20:43.17 delay 1 second @08:20:44.19 AFV.select :holdingCoil @08:20:44.29 delay 1 second @08:20:45.32 AS.reconfigure ASnorm @08:20:45.37 AS.seek 0ml @08:21:36.08 AFV.select :carrierSolution @08:21:36.19 delay 2 seconds @08:21:38.22 AS.reconfigure ASconfig @08:21:38.27 AS.seek 1ml @08:22:36.11 delay 1 second @08:22:37.13 AFV.select :holdingCoil @08:22:37.23 delay 1 second @08:22:38.26 ATV.dial :waste @08:22:39.39 AS.reconfigure ASnorm @08:22:39.45 AS.seek 0.5ml @08:23:05.01 ATV.dial :waste2 @08:23:06.19 AS.seek 0ml @08:23:31.70 AS.coast @08:23:31.75 SS.coast @08:23:31.78 CC.coast @08:23:32.28 CS.coast @08:23:32.41 PC.coast @08:23:32.45 PS.coast @08:23:32.49 Forearm.coast @08:23:32.54 Elbow.coast @08:23:32.93 Gate.power :core,:OFF @08:23:32.95 Gate.power :raw,:OFF @08:23:32.96 Shutdown @08:23:32.97 GoodNews.email "MFBPCR completed",Subject: "Shutdown" @08:23:33.00 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @08:23:33.02 Retry #1 of 10 after 10:00 @08:33:33.04 GoodNews.email "MFBPCR completed",Subject: "Shutdown" @08:33:33.07 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @08:33:33.08 Retry #2 of 10 after 10:00 @08:43:33.11 GoodNews.email "MFBPCR completed",Subject: "Shutdown" @08:43:33.14 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @08:43:33.15 Retry #3 of 10 after 10:00 @08:53:33.18 GoodNews.email "MFBPCR completed",Subject: "Shutdown" @08:53:33.21 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @08:53:33.22 Retry #4 of 10 after 10:00 @09:03:33.25 GoodNews.email "MFBPCR completed",Subject: "Shutdown" @09:03:33.28 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @09:03:33.29 Retry #5 of 10 after 10:00 @09:13:33.32 GoodNews.email "MFBPCR completed",Subject: "Shutdown" @09:13:33.35 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @09:13:33.36 Retry #6 of 10 after 10:00 @09:23:33.39 GoodNews.email "MFBPCR completed",Subject: "Shutdown" @09:23:33.42 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @09:23:33.43 Retry #7 of 10 after 10:00 @09:33:33.46 GoodNews.email "MFBPCR completed",Subject: "Shutdown" @09:33:33.49 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @09:33:33.50 Retry #8 of 10 after 10:00 @09:43:33.53 GoodNews.email "MFBPCR completed",Subject: "Shutdown" @09:43:33.56 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @09:43:33.57 Retry #9 of 10 after 10:00 @09:53:33.60 GoodNews.email "MFBPCR completed",Subject: "Shutdown" @09:53:33.63 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @09:53:33.64 Retry #10 of 10 after 10:00 @10:03:33.67 GoodNews.email "MFBPCR completed",Subject: "Shutdown" @10:03:33.70 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @20:43:00.04
Powering core bus @20:43:04.95 Gate.power :raw,:ON @20:43:05.57 SPE.coast @20:43:05.63 SPE.configure SPEconfig @20:43:05.72 AS.configure ASconfig @20:43:05.82 SS.configure SSconfig @20:43:05.91 CC.configure CCconfig @20:43:06.00 CS.configure CSconfig @20:43:06.10 PC.configure PCconfig @20:43:06.19 PS.configure PSconfig @20:43:06.31 Forearm.configure LooseForearm @20:43:06.40 Elbow.configure LooseElbow @20:43:06.52 SC.configure SCloose @20:43:06.61 SE.configure SEconfig @20:43:06.66 CH.coast @20:43:06.71 CH.configure CHconfig @20:43:06.74 PH.coast @20:43:06.80 PH.configure PHconfig @20:43:06.83 PCR.off @20:43:06.86 PCR.pidtemp= 50,0.1,0,300,25,25 @20:43:06.90 PCR.caltemp= 2.8814,0.0372,0 @20:43:06.92 All dwarf controllers running firmware version 3.75 @20:43:07.04 Valve.reset @20:43:07.20 PCR.off @20:43:07.23 PCR.pidtemp= 50,0.1,0,300,25,25 @20:43:07.25 PCR.caltemp= 2.8814,0.0372,0 @20:43:07.38 Intake.select :close @20:43:07.47 Exhaust.select :close @20:43:07.48 CTV.dial :air @20:43:07.57 PSV.dial :air @20:43:07.60 CSV.dial :waste1 @20:43:07.74
Forearm.reconfigure LooseForearm @20:43:07.78 Forearm.home.seek :retract @20:43:08.13 PS.reconfigure PSconfig @20:43:08.19 PS.home.seek 0ml @20:43:08.21 SSV.dial :spit,avoiding: :exhaust @20:43:08.50 Hand.close @20:43:08.53
Elbow.jog 1500 @20:43:09.08 CS.reconfigure CSfast @20:43:09.12 SS.reconfigure SSconfig @20:43:09.21 CS.home.seek 0ml @20:43:09.24 SS.home.seek 0ml @20:43:09.80
Elbow.reconfigure LooseElbow @20:43:09.92 Elbow.home.seek :clear @20:43:13.24 SE.reconfigure SEconfig @20:43:13.29 SE.home.seek :down @20:43:13.97 SC.reconfigure SCsloppy @20:43:14.02 SC.home.seek :initial @20:43:16.19 Forearm.seek :retract @20:43:16.26 Elbow.seek :hammer @20:43:17.16 PS.coast @20:43:17.57
Forearm.reconfigure TightForearm @20:43:17.62 Forearm.seek 5 + 350 counts @20:43:18.69 Forearm.seek :retract @20:43:19.82 PC.reconfigure PCconfig @20:43:19.85
CC.reconfigure CCconfig @20:43:19.94 PC.home.seek :open @20:43:19.96
CC.home.seek :open @20:43:23.41 Phase [wcrPhase2] @20:43:23.42 GoodNews.email "At 20:43:23 began in tube 2: wcrPhase2\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:129\n{1 => 12, 2 => 10}\nCan@20:43:23, 16.4C, 79% humidity, 14.6psia, 14.252V, 0.840A, 0.840A avg, 11.97W",Subject: "Started TotaldeploymentFINAL3 phase wcrPhase2" @20:43:23.44CEST12-Sep-19
==================> STARTED WCRSphase (phase #3) <=== @20:43:23.47 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @20:43:23.49 Starting initialPurge @20:43:23.51 Retry #1 of 10 after 10:00 @20:43:23.53
Loading FlushPuck into Collection Clamp from FlushPuck::Garage @20:43:23.60 Hand.open @20:43:24.31 Elbow.reconfigure TightElbow @20:43:24.36 Elbow.seek FlushPuck::Garage @20:43:26.19 Elbow.reconfigure LooseElbow @20:43:26.29 Forearm.reconfigure LooseForearm @20:43:26.33 Forearm.seek :garage @20:43:27.33 Hand.close @20:43:28.07 Forearm.seek :retract @20:43:29.05 Elbow.reconfigure TightElbow @20:43:29.10 Elbow.seek CC @20:43:31.97 Elbow.reconfigure LooseElbow @20:43:32.07 Forearm.reconfigure TightForearm @20:43:32.11 Forearm.seek CC @20:43:33.44 CC.seek :closed @20:43:37.70 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.70 @20:43:37.78 Hand.open @20:43:38.50 Forearm.reconfigure LooseForearm @20:43:38.55 Forearm.seek :retract @20:43:39.58 Flushing the Collection Valve::Manifold @20:43:39.71 CSV.dial CSR @20:43:41.05 Pulling 5ml of CSR.flush @20:43:41.06 CSR.select :flush @20:43:41.18 CS.reconfigure CSconfig @20:43:41.23 CS.seek 5ml @20:43:54.93 delay 5 seconds @20:43:59.95 CSR.select :air @20:44:00.12 CS.reconfigure CSfast @20:44:00.16 CS.seek 7.5ml @20:44:06.73 CSV.dial :waste @20:44:08.13 CS.reconfigure CSconfig @20:44:08.18 CS.seek 0ml @20:44:28.30 Purging external Intake @20:44:28.42 CSV.dial :flush @20:44:28.88 CS.reconfigure CSfast @20:44:28.93 CS.seek 10ml @20:44:51.68 delay 5 seconds @20:44:56.70 CSV.dial CBV @20:44:56.73
CBV.dial CSV @20:44:57.52 CTV.dial :intake @20:44:58.27 Intake.select :open @20:44:58.39 CS.seek 0ml @20:45:22.26 Intake.select :close @20:45:22.37 CTV.dial :air @20:45:23.13 Flushing upper manifold @20:45:23.25 CSV.dial :flush @20:45:24.01 CS.seek 5ml @20:45:35.87 delay 5 seconds @20:45:40.89 CSV.dial :air @20:45:41.39 CS.seek 7ml @20:45:46.90 CSV.dial CBV @20:45:46.92
CBV.dial CSV @20:45:47.96 CTV.dial :air @20:45:47.98 CS.seek 0ml @20:46:04.07 delay 7 seconds @20:46:11.11 CS.seek 3ml @20:46:18.64 CSV.dial :waste @20:46:19.11 CS.seek 0ml @20:46:26.79 CSV.dial :flush @20:46:27.25 CS.seek 3ml @20:46:34.96 delay 5 seconds @20:46:40.47 CSV.dial :air @20:46:41.00 CS.seek 5.001ml @20:46:46.43 CSV.dial :air @20:46:46.45 CS.seek 0ml @20:46:58.27 delay 7 seconds @20:47:05.31 CS.seek 3ml @20:47:12.89 CSV.dial :waste @20:47:13.64 CS.seek 0ml @20:47:21.12 Garaging FlushPuck in Collection Clamp @20:47:21.19 Elbow.reconfigure TightElbow @20:47:21.23 Elbow.seek CC @20:47:21.51 Elbow.reconfigure LooseElbow @20:47:21.61 Forearm.seek CC @20:47:22.63 Hand.close @20:47:23.20 CC.seek :open @20:47:27.55 Forearm.seek :retract @20:47:28.57 Elbow.reconfigure TightElbow @20:47:28.62 Elbow.seek :garage @20:47:31.60 Elbow.reconfigure LooseElbow @20:47:31.70 Forearm.reconfigure TightForearm @20:47:31.74 Forearm.seek :garage @20:47:32.94 Hand.open @20:47:33.63 Forearm.reconfigure LooseForearm @20:47:33.68 Forearm.seek :retract @20:47:34.67 Starting stand-alone bac WCR @20:47:34.69 bac wcr sampling at most 2000ml @20:47:34.71 Whole Cell Archive sampling 2000ml @20:47:34.73 Loading wcr puck from tube 2 into Collection Clamp @20:47:35.02 SC.reconfigure SCloose @20:47:35.24 Elbow.reconfigure TightElbow @20:47:35.29 Elbow.seek 2 @20:47:37.34 Forearm.reconfigure TightForearm @20:47:37.39 Forearm.seek 2 + 700 counts @20:47:38.45 SE.reconfigure SEpick @20:47:38.53 SE.seek beamBreak @20:47:40.65 SE.configure SEconfig @20:47:40.82 Elbow.seek 2 @20:47:41.01 Forearm.seek 2 + 300 counts @20:47:41.28 Forearm.reconfigure LooseForearm @20:47:41.32 Hand.close @20:47:41.96 SE.seek :down @20:47:42.18 Forearm.seek :retract @20:47:43.13 Elbow.seek CC @20:47:43.97 SE.coast @20:47:44.79 Elbow.reconfigure LooseElbow @20:47:44.90 Forearm.reconfigure TightForearm @20:47:44.95 Forearm.seek CC @20:47:46.29 CC.seek :closed @20:47:50.55 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.75 @20:47:50.56 Hand.open @20:47:51.27 Forearm.reconfigure LooseForearm @20:47:51.31 Forearm.seek :retract @20:47:52.32 SC.coast @20:47:52.36 collectSample 2000ml w/bubble point of 55psi @20:47:52.37 Priming 7ml @20:47:52.38 Intake.select :close @20:47:52.39 Exhaust.select :close @20:47:52.41 SS.hold @20:47:52.63 Calibrating pressure and temperature sensors @20:47:52.65 CTV.dial :air @20:47:52.67 SSV.dial :spit,avoiding: :exhaust @20:47:52.69 SS.reconfigure SSfast @20:47:52.73 SS.seek 5ml @20:47:55.21 SSV.dial CBV @20:47:55.23 CBV.dial SSV @20:47:56.57 delay 3.8 seconds @20:48:00.50 SSV.dial :spit @20:48:01.82 SS.seek 0ml @20:48:04.38 SSV.dial CBV @20:48:05.68 CTV.dial :intake @20:48:06.46 SSV.dial CBV @20:48:06.48 CBV.dial SSV @20:48:06.51 Allowing 40s for 7.0ml of remaining 7.0ml @20:48:06.52 Intake.select :open @20:48:08.69 Intake @32.2psia, vacuum from 20.0 to 24.0psi @20:48:08.71 SS.reconfigure SSinhale.with maxSpeed: 150 @20:48:08.76 SS.seek 7ml @20:48:12.66 Vacuum too high -- waiting up to 40s @20:48:15.66 Intake @31.8psia, vacuum from 20.0 to 24.0psi @20:48:15.68 SS.reconfigure SSinhale.with maxSpeed: 135 @20:48:15.73 SS.seek 7ml @20:48:18.38 Stroke completed -- waiting for pressure to equalize ... @20:48:22.79 Intake.select :close @20:48:22.93 SSV.dial :exhaust @20:48:23.71 Exhaust.select :open @20:48:23.84 SS.reconfigure SSexhale @20:48:23.91 SS.seek 0ml @20:48:27.56 Exhaust.select :close @20:48:27.68 Sampling 2000.0ml @20:48:27.69 Intake.select :close @20:48:27.70 Exhaust.select :close @20:48:27.72 SS.reconfigure SSconfig @20:48:27.77 SS.hold @20:48:27.98 CTV.dial :intake @20:48:28.00 SSV.dial CBV @20:48:28.02 CBV.dial SSV @20:48:29.32 Allowing 130s for 25.0ml of remaining 2000.0ml @20:48:29.34 Intake.select :open @20:48:31.51 Intake @32.5psia, vacuum from 20.0 to 24.0psi @20:48:31.53 SS.reconfigure SSinhale.with maxSpeed: 135 @20:48:31.58 SS.seek 25ml @20:48:35.19 Vacuum too high -- waiting up to 40s @20:48:37.01 Intake @31.8psia, vacuum from 20.0 to 24.0psi @20:48:37.03 SS.reconfigure SSinhale.with maxSpeed: 122 @20:48:37.08 SS.seek 25ml @20:48:40.13 Vacuum too high -- waiting up to 40s @20:48:42.16 Intake @31.7psia, vacuum from 20.0 to 24.0psi @20:48:42.18 SS.reconfigure SSinhale.with maxSpeed: 110 @20:48:42.23 SS.seek 25ml @20:48:45.60 Vacuum too high -- waiting up to 40s @20:48:47.37 Intake @31.5psia, vacuum from 20.0 to 24.0psi @20:48:47.39 SS.reconfigure SSinhale.with maxSpeed: 99 @20:48:47.44 SS.seek 25ml @20:48:50.67 Vacuum too high -- waiting up to 40s @20:48:52.45 Intake @31.8psia, vacuum from 20.0 to 24.0psi @20:48:52.47 SS.reconfigure SSinhale.with maxSpeed: 89 @20:48:52.52 SS.seek 25ml @20:48:55.73 Vacuum too high -- waiting up to 40s @20:48:57.50 Intake @31.7psia, vacuum from 20.0 to 24.0psi @20:48:57.52 SS.reconfigure SSinhale.with maxSpeed: 80 @20:48:57.57 SS.seek 25ml @20:49:01.42 Vacuum too high -- waiting up to 40s @20:49:03.71 Intake @31.9psia, vacuum from 20.0 to 24.0psi @20:49:03.73 SS.reconfigure SSinhale.with maxSpeed: 72 @20:49:03.77 SS.seek 25ml @20:49:07.94 Stroke completed -- waiting for pressure to equalize ... @20:49:11.76 Intake.select :close @20:49:11.92 SSV.dial :exhaust @20:49:12.68 Exhaust.select :open @20:49:12.79 SS.reconfigure SSexhale @20:49:12.84 SS.seek 0ml @20:49:21.69 Exhaust.select :close @20:49:21.81 SSV.dial CBV @20:49:21.83 CBV.dial SSV @20:49:22.62 Allowing 130s for 25.0ml of remaining 1975.0ml @20:49:22.63 Intake.select :open @20:49:24.83 Intake @32.2psia, vacuum from 20.0 to 24.0psi @20:49:24.85 SS.reconfigure SSinhale.with maxSpeed: 72 @20:49:24.90 SS.seek 25ml @20:49:30.16 Vacuum too high -- waiting up to 40s @20:49:31.52 Intake @31.5psia, vacuum from 20.0 to 24.0psi @20:49:31.54 SS.reconfigure SSinhale.with maxSpeed: 65 @20:49:31.59 SS.seek 25ml @20:49:37.82 Vacuum too high -- waiting up to 40s @20:49:39.35 Intake @31.6psia, vacuum from 20.0 to 24.0psi @20:49:39.37 SS.reconfigure SSinhale.with maxSpeed: 59 @20:49:39.42 SS.seek 25ml @20:49:46.63 Vacuum too high -- waiting up to 40s @20:49:48.15 Intake @31.8psia, vacuum from 20.0 to 24.0psi @20:49:48.17 SS.reconfigure SSinhale.with maxSpeed: 53 @20:49:48.22 SS.seek 25ml @20:49:57.57 Vacuum too high -- waiting up to 40s @20:49:59.11 Intake @31.8psia, vacuum from 20.0 to 24.0psi @20:49:59.13 SS.reconfigure SSinhale.with maxSpeed: 48 @20:49:59.18 SS.seek 25ml @20:50:03.33 Stroke completed -- waiting for pressure to equalize ... @20:50:06.50 Intake.select :close @20:50:07.16 SSV.dial :exhaust @20:50:07.91 Exhaust.select :open @20:50:08.02 SS.reconfigure SSexhale @20:50:08.07 SS.seek 0ml @20:50:17.11 Exhaust.select :close @20:50:17.23 SSV.dial CBV @20:50:17.25 CBV.dial SSV @20:50:18.04 Allowing 130s for 25.0ml of remaining 1950.0ml @20:50:18.05 Intake.select :open @20:50:20.22 Intake @32.7psia, vacuum from 20.0 to 24.0psi @20:50:20.24 SS.reconfigure SSinhale.with maxSpeed: 48 @20:50:20.29 SS.seek 25ml @20:50:31.69 Vacuum too high -- waiting up to 40s @20:50:32.99 Intake @32.2psia, vacuum from 20.0 to 24.0psi @20:50:33.01 SS.reconfigure SSinhale.with maxSpeed: 43 @20:50:33.06 SS.seek 25ml @20:50:51.41 Vacuum too high -- waiting up to 40s @20:50:52.98 SS.reconfigure SSinhale.with maxSpeed: 39 @20:50:53.03 SS.seek 25ml @20:51:05.51 Stroke completed -- waiting for pressure to equalize ... @20:51:09.31 Intake.select :close @20:51:09.45 SSV.dial :exhaust @20:51:10.22 Exhaust.select :open @20:51:10.34 SS.reconfigure SSexhale @20:51:10.38 SS.seek 0ml @20:51:19.39 Exhaust.select :close @20:51:19.51 SSV.dial CBV @20:51:19.53 CBV.dial SSV @20:51:20.32 Allowing 130s for 25.0ml of remaining 1925.0ml @20:51:20.33 Intake.select :open @20:51:22.50 Intake @32.7psia, vacuum from 20.0 to 24.0psi @20:51:22.51 SS.reconfigure SSinhale.with maxSpeed: 39 @20:51:22.57 SS.seek 25ml @20:51:49.10 Vacuum too high -- waiting up to 40s @20:51:50.41 Intake @31.9psia, vacuum from 20.0 to 24.0psi @20:51:50.43 SS.reconfigure SSinhale.with maxSpeed: 35 @20:51:50.47 SS.seek 25ml @20:52:11.47 Stroke completed -- waiting for pressure to equalize ... @20:52:15.88 Intake.select :close @20:52:16.03 SSV.dial :exhaust @20:52:16.80 Exhaust.select :open @20:52:16.94 SS.reconfigure SSexhale @20:52:17.49 SS.seek 0ml @20:52:26.21 Exhaust.select :close @20:52:26.32 SSV.dial CBV @20:52:26.34 CBV.dial SSV @20:52:27.15 Allowing 130s for 25.0ml of remaining 1900.0ml @20:52:27.16 Intake.select :open @20:52:29.33 Intake @32.5psia, vacuum from 20.0 to 24.0psi @20:52:29.35 SS.reconfigure SSinhale.with maxSpeed: 35 @20:52:29.40 SS.seek 25ml @20:53:19.90 Stroke completed -- waiting for pressure to equalize ... @20:53:23.53 GoodNews.email "At 20:43:23 began in tube 2: wcrPhase2\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:129\n{1 => 12, 2 => 10}\nCan@20:43:23, 16.4C, 79% humidity, 14.6psia, 14.252V, 0.840A, 0.840A avg, 11.97W",Subject: "Started TotaldeploymentFINAL3 phase wcrPhase2" @20:53:23.56 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @20:53:23.57 Retry #2 of 10 after 10:00 @20:53:24.39 Intake.select :close @20:53:24.54 SSV.dial :exhaust @20:53:25.33 Exhaust.select :open @20:53:25.44 SS.reconfigure SSexhale @20:53:25.49 SS.seek 0ml @20:53:34.47 Exhaust.select :close @20:53:34.59 SSV.dial CBV @20:53:34.62 CBV.dial SSV @20:53:35.41 Allowing 130s for 25.0ml of remaining 1875.0ml @20:53:35.42 Intake.select :open @20:53:37.59 Intake @32.5psia, vacuum from 20.0 to 24.0psi @20:53:37.61 SS.reconfigure SSinhale.with maxSpeed: 35 @20:53:37.66 SS.seek 25ml @20:54:28.01 Stroke completed -- waiting for pressure to equalize ... @20:54:32.42 Intake.select :close @20:54:32.58 SSV.dial :exhaust @20:54:33.36 Exhaust.select :open @20:54:33.47 SS.reconfigure SSexhale @20:54:33.52 SS.seek 0ml @20:54:42.67 Exhaust.select :close @20:54:42.79 SSV.dial CBV @20:54:42.81 CBV.dial SSV @20:54:43.62 Allowing 130s for 25.0ml of remaining 1850.0ml @20:54:43.63 Intake.select :open @20:54:45.80 Intake @32.1psia, vacuum from 20.0 to 24.0psi @20:54:45.82 SS.reconfigure SSinhale.with maxSpeed: 35 @20:54:45.86 SS.seek 25ml @20:55:36.20 Stroke completed -- waiting for pressure to equalize ... @20:55:40.61 Intake.select :close @20:55:40.83 SSV.dial :exhaust @20:55:41.59 Exhaust.select :open @20:55:41.70 SS.reconfigure SSexhale @20:55:41.75 SS.seek 0ml @20:55:50.64 Exhaust.select :close @20:55:50.76 SSV.dial CBV @20:55:50.79 CBV.dial SSV @20:55:51.57 Allowing 130s for 25.0ml of remaining 1825.0ml @20:55:51.58 Intake.select :open @20:55:53.75 Intake @32.5psia, vacuum from 20.0 to 24.0psi @20:55:53.77 SS.reconfigure SSinhale.with maxSpeed: 35 @20:55:53.81 SS.seek 25ml @20:56:44.11 Stroke completed -- waiting for pressure to equalize ... @20:56:47.79 Intake.select :close @20:56:47.94 SSV.dial :exhaust @20:56:48.71 Exhaust.select :open @20:56:48.83 SS.reconfigure SSexhale @20:56:48.88 SS.seek 0ml @20:56:58.00 Exhaust.select :close @20:56:58.16 SSV.dial CBV @20:56:58.20 CBV.dial SSV @20:56:59.04 Allowing 130s for 25.0ml of remaining 1800.0ml @20:56:59.05 Intake.select :open @20:57:01.22 Intake @32.2psia, vacuum from 20.0 to 24.0psi @20:57:01.24 SS.reconfigure SSinhale.with maxSpeed: 35 @20:57:01.29 SS.seek 25ml @20:57:51.67 Stroke completed -- waiting for pressure to equalize ... @20:57:55.47 Intake.select :close @20:57:55.62 SSV.dial :exhaust @20:57:56.38 Exhaust.select :open @20:57:56.49 SS.reconfigure SSexhale @20:57:56.54 SS.seek 0ml @20:58:05.29 Exhaust.select :close @20:58:05.41 SSV.dial CBV @20:58:05.43 CBV.dial SSV @20:58:06.24 Allowing 130s for 25.0ml of remaining 1775.0ml @20:58:06.25 Intake.select :open @20:58:08.42 Intake @32.3psia, vacuum from 20.0 to 24.0psi @20:58:08.44 SS.reconfigure SSinhale.with maxSpeed: 35 @20:58:08.48 SS.seek 25ml @20:58:59.09 Stroke completed -- waiting for pressure to equalize ... @20:59:02.26 Intake.select :close @20:59:02.41 SSV.dial :exhaust @20:59:03.18 Exhaust.select :open @20:59:03.30 SS.reconfigure SSexhale @20:59:03.35 SS.seek 0ml @20:59:12.43 Exhaust.select :close @20:59:12.55 SSV.dial CBV @20:59:12.57 CBV.dial SSV @20:59:13.38 Allowing 130s for 25.0ml of remaining 1750.0ml @20:59:13.39 Intake.select :open @20:59:15.56 Intake @32.4psia, vacuum from 20.0 to 24.0psi @20:59:15.57 SS.reconfigure SSinhale.with maxSpeed: 35 @20:59:15.62 SS.seek 25ml @21:00:05.67 Stroke completed -- waiting for pressure to equalize ... @21:00:08.83 Intake.select :close @21:00:08.98 SSV.dial :exhaust @21:00:09.75 Exhaust.select :open @21:00:09.87 SS.reconfigure SSexhale @21:00:09.93 SS.seek 0ml @21:00:19.09 Exhaust.select :close @21:00:19.21 SSV.dial CBV @21:00:19.23 CBV.dial SSV @21:00:20.04 Allowing 130s for 25.0ml of remaining 1725.0ml @21:00:20.05 Intake.select :open @21:00:22.22 Intake @32.6psia, vacuum from 20.0 to 24.0psi @21:00:22.24 SS.reconfigure SSinhale.with maxSpeed: 35 @21:00:22.28 SS.seek 25ml @21:01:13.15 Stroke completed -- waiting for pressure to equalize ... @21:01:16.32 Intake.select :close @21:01:16.47 SSV.dial :exhaust @21:01:17.23 Exhaust.select :open @21:01:17.35 SS.reconfigure SSexhale @21:01:17.40 SS.seek 0ml @21:01:26.15 Exhaust.select :close @21:01:26.27 SSV.dial CBV @21:01:26.29 CBV.dial SSV @21:01:27.08 Allowing 130s for 25.0ml of remaining 1700.0ml @21:01:27.09 Intake.select :open @21:01:29.26 Intake @32.6psia, vacuum from 20.0 to 24.0psi @21:01:29.28 SS.reconfigure SSinhale.with maxSpeed: 35 @21:01:29.32 SS.seek 25ml @21:02:19.87 Stroke completed -- waiting for pressure to equalize ... @21:02:23.04 Intake.select :close @21:02:23.20 SSV.dial :exhaust @21:02:23.96 Exhaust.select :open @21:02:24.07 SS.reconfigure SSexhale @21:02:24.12 SS.seek 0ml @21:02:32.76 Exhaust.select :close @21:02:32.88 SSV.dial CBV @21:02:32.90 CBV.dial SSV @21:02:33.71 Allowing 130s for 25.0ml of remaining 1675.0ml @21:02:33.72 Intake.select :open @21:02:35.88 Intake @32.7psia, vacuum from 20.0 to 24.0psi @21:02:35.90 SS.reconfigure SSinhale.with maxSpeed: 35 @21:02:35.95 SS.seek 25ml @21:03:23.60 GoodNews.email "At 20:43:23 began in tube 2: wcrPhase2\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:129\n{1 => 12, 2 => 10}\nCan@20:43:23, 16.4C, 79% humidity, 14.6psia, 14.252V, 0.840A, 0.840A avg, 11.97W",Subject: "Started TotaldeploymentFINAL3 phase wcrPhase2" @21:03:23.63 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @21:03:23.64 Retry #3 of 10 after 10:00 @21:03:26.57 Stroke completed -- waiting for pressure to equalize ... @21:03:29.75 Intake.select :close @21:03:29.90 SSV.dial :exhaust @21:03:30.67 Exhaust.select :open @21:03:30.79 SS.reconfigure SSexhale @21:03:30.83 SS.seek 0ml @21:03:39.75 Exhaust.select :close @21:03:39.87 SSV.dial CBV @21:03:39.89 CBV.dial SSV @21:03:40.69 Allowing 130s for 25.0ml of remaining 1650.0ml @21:03:40.70 Intake.select :open @21:03:42.87 Intake @32.6psia, vacuum from 20.0 to 24.0psi @21:03:42.89 SS.reconfigure SSinhale.with maxSpeed: 35 @21:03:42.93 SS.seek 25ml @21:04:33.28 Stroke completed -- waiting for pressure to equalize ... @21:04:37.09 Intake.select :close @21:04:37.24 SSV.dial :exhaust @21:04:38.00 Exhaust.select :open @21:04:38.12 SS.reconfigure SSexhale @21:04:38.18 SS.seek 0ml @21:04:47.20 Exhaust.select :close @21:04:47.32 SSV.dial CBV @21:04:47.34 CBV.dial SSV @21:04:48.65 Allowing 130s for 25.0ml of remaining 1625.0ml @21:04:48.66 Intake.select :open @21:04:50.82 Intake @32.6psia, vacuum from 20.0 to 24.0psi @21:04:50.84 SS.reconfigure SSinhale.with maxSpeed: 35 @21:04:50.89 SS.seek 25ml @21:05:40.96 Stroke completed -- waiting for pressure to equalize ... @21:05:44.75 Intake.select :close @21:05:44.91 SSV.dial :exhaust @21:05:45.68 Exhaust.select :open @21:05:45.80 SS.reconfigure SSexhale @21:05:45.85 SS.seek 0ml @21:05:54.75 Exhaust.select :close @21:05:54.87 SSV.dial CBV @21:05:54.89 CBV.dial SSV @21:05:55.69 Allowing 130s for 25.0ml of remaining 1600.0ml @21:05:55.70 Intake.select :open @21:05:57.87 Intake @32.7psia, vacuum from 20.0 to 24.0psi @21:05:57.89 SS.reconfigure SSinhale.with maxSpeed: 35 @21:05:57.94 SS.seek 25ml @21:06:48.28 Stroke completed -- waiting for pressure to equalize ... @21:06:52.07 Intake.select :close @21:06:52.22 SSV.dial :exhaust @21:06:52.99 Exhaust.select :open @21:06:53.10 SS.reconfigure SSexhale @21:06:53.15 SS.seek 0ml @21:07:02.06 Exhaust.select :close @21:07:02.18 SSV.dial CBV @21:07:02.20 CBV.dial SSV @21:07:02.99 Allowing 130s for 25.0ml of remaining 1575.0ml @21:07:03.00 Intake.select :open @21:07:05.17 Intake @32.9psia, vacuum from 20.0 to 24.0psi @21:07:05.19 SS.reconfigure SSinhale.with maxSpeed: 35 @21:07:05.23 SS.seek 25ml @21:07:55.53 Stroke completed -- waiting for pressure to equalize ... @21:07:59.32 Intake.select :close @21:07:59.47 SSV.dial :exhaust @21:08:00.22 Exhaust.select :open @21:08:00.33 SS.reconfigure SSexhale @21:08:00.38 SS.seek 0ml @21:08:09.46 Exhaust.select :close @21:08:09.58 SSV.dial CBV @21:08:09.60 CBV.dial SSV @21:08:10.40 Allowing 130s for 25.0ml of remaining 1550.0ml @21:08:10.41 Intake.select :open @21:08:12.58 Intake @32.6psia, vacuum from 20.0 to 24.0psi @21:08:12.60 SS.reconfigure SSinhale.with maxSpeed: 35 @21:08:12.64 SS.seek 25ml @21:09:03.04 Stroke completed -- waiting for pressure to equalize ... @21:09:06.95 Intake.select :close @21:09:07.10 SSV.dial :exhaust @21:09:08.36 Exhaust.select :open @21:09:08.47 SS.reconfigure SSexhale @21:09:08.52 SS.seek 0ml @21:09:17.21 Exhaust.select :close @21:09:17.33 SSV.dial CBV @21:09:17.35 CBV.dial SSV @21:09:18.16 Allowing 130s for 25.0ml of remaining 1525.0ml @21:09:18.17 Intake.select :open @21:09:20.34 Intake @32.7psia, vacuum from 20.0 to 24.0psi @21:09:20.36 SS.reconfigure SSinhale.with maxSpeed: 35 @21:09:20.41 SS.seek 25ml @21:10:11.06 Stroke completed -- waiting for pressure to equalize ... @21:10:14.84 Intake.select :close @21:10:14.99 SSV.dial :exhaust @21:10:15.76 Exhaust.select :open @21:10:15.88 SS.reconfigure SSexhale @21:10:15.93 SS.seek 0ml @21:10:24.98 Exhaust.select :close @21:10:25.10 SSV.dial CBV @21:10:25.12 CBV.dial SSV @21:10:25.93 Allowing 130s for 25.0ml of remaining 1500.0ml @21:10:25.94 Intake.select :open @21:10:28.11 Intake @32.7psia, vacuum from 20.0 to 24.0psi @21:10:28.13 SS.reconfigure SSinhale.with maxSpeed: 35 @21:10:28.17 SS.seek 25ml @21:11:18.64 Stroke completed -- waiting for pressure to equalize ... @21:11:22.43 Intake.select :close @21:11:22.58 SSV.dial :exhaust @21:11:23.36 Exhaust.select :open @21:11:23.47 SS.reconfigure SSexhale @21:11:23.52 SS.seek 0ml @21:11:32.40 Exhaust.select :close @21:11:32.51 SSV.dial CBV @21:11:32.53 CBV.dial SSV @21:11:33.33 Allowing 130s for 25.0ml of remaining 1475.0ml @21:11:33.34 Intake.select :open @21:11:35.51 Intake @32.8psia, vacuum from 20.0 to 24.0psi @21:11:35.53 SS.reconfigure SSinhale.with maxSpeed: 35 @21:11:35.57 SS.seek 25ml @21:12:25.96 Stroke completed -- waiting for pressure to equalize ... @21:12:29.75 Intake.select :close @21:12:29.90 SSV.dial :exhaust @21:12:30.68 Exhaust.select :open @21:12:30.80 SS.reconfigure SSexhale @21:12:30.85 SS.seek 0ml @21:12:39.73 Exhaust.select :close @21:12:39.85 SSV.dial CBV @21:12:39.87 CBV.dial SSV @21:12:40.68 Allowing 130s for 25.0ml of remaining 1450.0ml @21:12:40.69 Intake.select :open @21:12:42.86 Intake @32.8psia, vacuum from 20.0 to 24.0psi @21:12:42.88 SS.reconfigure SSinhale.with maxSpeed: 35 @21:12:42.92 SS.seek 25ml @21:13:23.67 GoodNews.email "At 20:43:23 began in tube 2: wcrPhase2\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:129\n{1 => 12, 2 => 10}\nCan@20:43:23, 16.4C, 79% humidity, 14.6psia, 14.252V, 0.840A, 0.840A avg, 11.97W",Subject: "Started TotaldeploymentFINAL3 phase wcrPhase2" @21:13:23.70 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @21:13:23.71 Retry #4 of 10 after 10:00 @21:13:33.15 Stroke completed -- waiting for pressure to equalize ... @21:13:36.92 Intake.select :close @21:13:37.08 SSV.dial :exhaust @21:13:37.83 Exhaust.select :open @21:13:37.95 SS.reconfigure SSexhale @21:13:37.99 SS.seek 0ml @21:13:47.23 Exhaust.select :close @21:13:47.34 SSV.dial CBV @21:13:47.36 CBV.dial SSV @21:13:48.14 Allowing 130s for 25.0ml of remaining 1425.0ml @21:13:48.15 Intake.select :open @21:13:50.32 Intake @32.7psia, vacuum from 20.0 to 24.0psi @21:13:50.34 SS.reconfigure SSinhale.with maxSpeed: 35 @21:13:50.39 SS.seek 25ml @21:14:40.84 Stroke completed -- waiting for pressure to equalize ... @21:14:44.65 Intake.select :close @21:14:44.84 SSV.dial :exhaust @21:14:45.61 Exhaust.select :open @21:14:45.72 SS.reconfigure SSexhale @21:14:45.77 SS.seek 0ml @21:14:54.68 Exhaust.select :close @21:14:54.80 SSV.dial CBV @21:14:54.82 CBV.dial SSV @21:14:55.61 Allowing 130s for 25.0ml of remaining 1400.0ml @21:14:55.62 Intake.select :open @21:14:57.79 Intake @32.8psia, vacuum from 20.0 to 24.0psi @21:14:57.81 SS.reconfigure SSinhale.with maxSpeed: 35 @21:14:57.86 SS.seek 25ml @21:15:48.21 Stroke completed -- waiting for pressure to equalize ... @21:15:52.62 Intake.select :close @21:15:52.77 SSV.dial :exhaust @21:15:53.55 Exhaust.select :open @21:15:53.66 SS.reconfigure SSexhale @21:15:53.71 SS.seek 0ml @21:16:02.79 Exhaust.select :close @21:16:02.91 SSV.dial CBV @21:16:02.93 CBV.dial SSV @21:16:03.72 Allowing 130s for 25.0ml of remaining 1375.0ml @21:16:03.73 Intake.select :open @21:16:05.90 Intake @33.3psia, vacuum from 20.0 to 24.0psi @21:16:05.92 SS.reconfigure SSinhale.with maxSpeed: 35 @21:16:05.96 SS.seek 25ml @21:16:55.88 Stroke completed -- waiting for pressure to equalize ... @21:17:00.29 Intake.select :close @21:17:00.44 SSV.dial :exhaust @21:17:01.22 Exhaust.select :open @21:17:01.33 SS.reconfigure SSexhale @21:17:01.38 SS.seek 0ml @21:17:10.34 Exhaust.select :close @21:17:10.50 SSV.dial CBV @21:17:10.52 CBV.dial SSV @21:17:11.80 Allowing 130s for 25.0ml of remaining 1350.0ml @21:17:11.81 Intake.select :open @21:17:13.98 Intake @32.7psia, vacuum from 20.0 to 24.0psi @21:17:14.00 SS.reconfigure SSinhale.with maxSpeed: 35 @21:17:14.04 SS.seek 25ml @21:18:04.46 Stroke completed -- waiting for pressure to equalize ... @21:18:08.99 Intake.select :close @21:18:09.15 SSV.dial :exhaust @21:18:09.91 Exhaust.select :open @21:18:10.02 SS.reconfigure SSexhale @21:18:10.07 SS.seek 0ml @21:18:18.74 Exhaust.select :close @21:18:18.86 SSV.dial CBV @21:18:18.88 CBV.dial SSV @21:18:19.69 Allowing 130s for 25.0ml of remaining 1325.0ml @21:18:19.70 Intake.select :open @21:18:21.87 Intake @32.9psia, vacuum from 20.0 to 24.0psi @21:18:21.89 SS.reconfigure SSinhale.with maxSpeed: 35 @21:18:21.94 SS.seek 25ml @21:19:12.60 Stroke completed -- waiting for pressure to equalize ... @21:19:17.03 Intake.select :close @21:19:17.18 SSV.dial :exhaust @21:19:18.13 Exhaust.select :open @21:19:18.24 SS.reconfigure SSexhale @21:19:18.29 SS.seek 0ml @21:19:26.99 Exhaust.select :close @21:19:27.11 SSV.dial CBV @21:19:27.13 CBV.dial SSV @21:19:27.94 Allowing 130s for 25.0ml of remaining 1300.0ml @21:19:27.95 Intake.select :open @21:19:30.12 Intake @32.7psia, vacuum from 20.0 to 24.0psi @21:19:30.14 SS.reconfigure SSinhale.with maxSpeed: 35 @21:19:30.19 SS.seek 25ml @21:20:20.85 Stroke completed -- waiting for pressure to equalize ... @21:20:25.26 Intake.select :close @21:20:25.41 SSV.dial :exhaust @21:20:26.19 Exhaust.select :open @21:20:26.30 SS.reconfigure SSexhale @21:20:26.36 SS.seek 0ml @21:20:35.09 Exhaust.select :close @21:20:35.21 SSV.dial CBV @21:20:35.24 CBV.dial SSV @21:20:36.03 Allowing 130s for 25.0ml of remaining 1275.0ml @21:20:36.04 Intake.select :open @21:20:38.21 Intake @32.9psia, vacuum from 20.0 to 24.0psi @21:20:38.23 SS.reconfigure SSinhale.with maxSpeed: 35 @21:20:38.28 SS.seek 25ml @21:21:28.96 Stroke completed -- waiting for pressure to equalize ... @21:21:33.90 Intake.select :close @21:21:34.07 SSV.dial :exhaust @21:21:34.85 Exhaust.select :open @21:21:34.96 SS.reconfigure SSexhale @21:21:35.01 SS.seek 0ml @21:21:43.70 Exhaust.select :close @21:21:43.82 SSV.dial CBV @21:21:43.84 CBV.dial SSV @21:21:44.64 Allowing 130s for 25.0ml of remaining 1250.0ml @21:21:44.65 Intake.select :open @21:21:46.82 Intake @33.0psia, vacuum from 20.0 to 24.0psi @21:21:46.84 SS.reconfigure SSinhale.with maxSpeed: 35 @21:21:46.89 SS.seek 25ml @21:22:37.60 Stroke completed -- waiting for pressure to equalize ... @21:22:42.76 Intake.select :close @21:22:42.91 SSV.dial :exhaust @21:22:43.66 Exhaust.select :open @21:22:43.78 SS.reconfigure SSexhale @21:22:43.83 SS.seek 0ml @21:22:52.68 Exhaust.select :close @21:22:52.80 SSV.dial CBV @21:22:52.83 CBV.dial SSV @21:22:53.63 Allowing 130s for 25.0ml of remaining 1225.0ml @21:22:53.64 Intake.select :open @21:22:55.81 Intake @33.0psia, vacuum from 20.0 to 24.0psi @21:22:55.83 SS.reconfigure SSinhale.with maxSpeed: 35 @21:22:55.88 SS.seek 25ml @21:23:23.74 GoodNews.email "At 20:43:23 began in tube 2: wcrPhase2\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:129\n{1 => 12, 2 => 10}\nCan@20:43:23, 16.4C, 79% humidity, 14.6psia, 14.252V, 0.840A, 0.840A avg, 11.97W",Subject: "Started TotaldeploymentFINAL3 phase wcrPhase2" @21:23:23.77 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @21:23:23.78 Retry #5 of 10 after 10:00 @21:23:46.62 Stroke completed -- waiting for pressure to equalize ... @21:23:51.66 Intake.select :close @21:23:51.81 SSV.dial :exhaust @21:23:52.60 Exhaust.select :open @21:23:52.71 SS.reconfigure SSexhale @21:23:52.76 SS.seek 0ml @21:24:01.76 Exhaust.select :close @21:24:01.88 SSV.dial CBV @21:24:01.90 CBV.dial SSV @21:24:02.71 Allowing 130s for 25.0ml of remaining 1200.0ml @21:24:02.72 Intake.select :open @21:24:04.89 Intake @33.0psia, vacuum from 20.0 to 24.0psi @21:24:04.91 SS.reconfigure SSinhale.with maxSpeed: 35 @21:24:04.95 SS.seek 25ml @21:24:55.24 Stroke completed -- waiting for pressure to equalize ... @21:25:00.34 Intake.select :close @21:25:00.49 SSV.dial :exhaust @21:25:01.77 Exhaust.select :open @21:25:01.88 SS.reconfigure SSexhale @21:25:01.93 SS.seek 0ml @21:25:10.93 Exhaust.select :close @21:25:11.05 SSV.dial CBV @21:25:11.08 CBV.dial SSV @21:25:11.88 Allowing 130s for 25.0ml of remaining 1175.0ml @21:25:11.89 Intake.select :open @21:25:14.06 Intake @32.9psia, vacuum from 20.0 to 24.0psi @21:25:14.08 SS.reconfigure SSinhale.with maxSpeed: 35 @21:25:14.13 SS.seek 25ml @21:26:04.52 Stroke completed -- waiting for pressure to equalize ... @21:26:10.20 Intake.select :close @21:26:10.36 SSV.dial :exhaust @21:26:11.13 Exhaust.select :open @21:26:11.24 SS.reconfigure SSexhale @21:26:11.29 SS.seek 0ml @21:26:20.04 Exhaust.select :close @21:26:20.16 SSV.dial CBV @21:26:20.19 CBV.dial SSV @21:26:20.99 Allowing 130s for 25.0ml of remaining 1150.0ml @21:26:21.00 Intake.select :open @21:26:23.17 Intake @33.1psia, vacuum from 20.0 to 24.0psi @21:26:23.19 SS.reconfigure SSinhale.with maxSpeed: 35 @21:26:23.24 SS.seek 25ml @21:27:13.91 Stroke completed -- waiting for pressure to equalize ... @21:27:19.56 Intake.select :close @21:27:19.71 SSV.dial :exhaust @21:27:20.47 Exhaust.select :open @21:27:20.58 SS.reconfigure SSexhale @21:27:20.63 SS.seek 0ml @21:27:29.63 Exhaust.select :close @21:27:29.74 SSV.dial CBV @21:27:29.76 CBV.dial SSV @21:27:30.55 Allowing 130s for 25.0ml of remaining 1125.0ml @21:27:30.56 Intake.select :open @21:27:32.73 Intake @33.1psia, vacuum from 20.0 to 24.0psi @21:27:32.75 SS.reconfigure SSinhale.with maxSpeed: 35 @21:27:32.80 SS.seek 25ml @21:28:20.28 Vacuum too high -- waiting up to 40s @21:28:21.57 Intake @32.6psia, vacuum from 20.0 to 24.0psi @21:28:21.59 SS.reconfigure SSinhale.with maxSpeed: 32 @21:28:21.64 SS.seek 25ml @21:28:25.41 Stroke completed -- waiting for pressure to equalize ... @21:28:30.31 Intake.select :close @21:28:30.48 SSV.dial :exhaust @21:28:31.24 Exhaust.select :open @21:28:31.35 SS.reconfigure SSexhale @21:28:31.40 SS.seek 0ml @21:28:40.07 Exhaust.select :close @21:28:40.19 SSV.dial CBV @21:28:40.22 CBV.dial SSV @21:28:41.04 Allowing 130s for 25.0ml of remaining 1100.0ml @21:28:41.09 Intake.select :open @21:28:43.32 Intake @33.1psia, vacuum from 20.0 to 24.0psi @21:28:43.36 SS.reconfigure SSinhale.with maxSpeed: 32 @21:28:43.45 SS.seek 25ml @21:29:38.88 Stroke completed -- waiting for pressure to equalize ... @21:29:43.94 Intake.select :close @21:29:44.10 SSV.dial :exhaust @21:29:44.88 Exhaust.select :open @21:29:44.99 SS.reconfigure SSexhale @21:29:45.04 SS.seek 0ml @21:29:53.85 Exhaust.select :close @21:29:53.97 SSV.dial CBV @21:29:53.99 CBV.dial SSV @21:29:54.80 Allowing 130s for 25.0ml of remaining 1075.0ml @21:29:54.81 Intake.select :open @21:29:56.98 Intake @33.2psia, vacuum from 20.0 to 24.0psi @21:29:57.00 SS.reconfigure SSinhale.with maxSpeed: 32 @21:29:57.05 SS.seek 25ml @21:30:52.38 Stroke completed -- waiting for pressure to equalize ... @21:30:58.03 Intake.select :close @21:30:58.18 SSV.dial :exhaust @21:30:58.94 Exhaust.select :open @21:30:59.05 SS.reconfigure SSexhale @21:30:59.10 SS.seek 0ml @21:31:08.27 Exhaust.select :close @21:31:08.39 SSV.dial CBV @21:31:08.41 CBV.dial SSV @21:31:09.20 Allowing 130s for 25.0ml of remaining 1050.0ml @21:31:09.21 Intake.select :open @21:31:11.38 Intake @32.9psia, vacuum from 20.0 to 24.0psi @21:31:11.39 SS.reconfigure SSinhale.with maxSpeed: 32 @21:31:11.44 SS.seek 25ml @21:32:06.30 Stroke completed -- waiting for pressure to equalize ... @21:32:11.33 Intake.select :close @21:32:11.48 SSV.dial :exhaust @21:32:12.25 Exhaust.select :open @21:32:12.86 SS.reconfigure SSexhale @21:32:12.91 SS.seek 0ml @21:32:21.83 Exhaust.select :close @21:32:21.95 SSV.dial CBV @21:32:21.98 CBV.dial SSV @21:32:22.77 Allowing 130s for 25.0ml of remaining 1025.0ml @21:32:22.78 Intake.select :open @21:32:24.95 Intake @33.2psia, vacuum from 20.0 to 24.0psi @21:32:24.97 SS.reconfigure SSinhale.with maxSpeed: 32 @21:32:25.02 SS.seek 25ml @21:33:20.04 Stroke completed -- waiting for pressure to equalize ... @21:33:23.81 GoodNews.email "At 20:43:23 began in tube 2: wcrPhase2\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:129\n{1 => 12, 2 => 10}\nCan@20:43:23, 16.4C, 79% humidity, 14.6psia, 14.252V, 0.840A, 0.840A avg, 11.97W",Subject: "Started TotaldeploymentFINAL3 phase wcrPhase2" @21:33:23.84 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @21:33:23.86 Retry #6 of 10 after 10:00 @21:33:25.71 Intake.select :close @21:33:25.86 SSV.dial :exhaust @21:33:26.62 Exhaust.select :open @21:33:26.74 SS.reconfigure SSexhale @21:33:26.80 SS.seek 0ml @21:33:35.90 Exhaust.select :close @21:33:36.02 SSV.dial CBV @21:33:36.04 CBV.dial SSV @21:33:36.83 Allowing 130s for 25.0ml of remaining 1000.0ml @21:33:36.84 Intake.select :open @21:33:39.01 Intake @33.0psia, vacuum from 20.0 to 24.0psi @21:33:39.02 SS.reconfigure SSinhale.with maxSpeed: 32 @21:33:39.07 SS.seek 25ml @21:34:34.30 Stroke completed -- waiting for pressure to equalize ... @21:34:39.33 Intake.select :close @21:34:39.48 SSV.dial :exhaust @21:34:40.25 Exhaust.select :open @21:34:40.36 SS.reconfigure SSexhale @21:34:40.41 SS.seek 0ml @21:34:49.19 Exhaust.select :close @21:34:49.31 SSV.dial CBV @21:34:49.33 CBV.dial SSV @21:34:50.14 Allowing 130s for 25.0ml of remaining 975.0ml @21:34:50.15 Intake.select :open @21:34:52.33 Intake @33.0psia, vacuum from 20.0 to 24.0psi @21:34:52.35 SS.reconfigure SSinhale.with maxSpeed: 32 @21:34:52.40 SS.seek 25ml @21:35:47.79 Stroke completed -- waiting for pressure to equalize ... @21:35:54.08 Intake.select :close @21:35:54.23 SSV.dial :exhaust @21:35:55.02 Exhaust.select :open @21:35:55.13 SS.reconfigure SSexhale @21:35:55.18 SS.seek 0ml @21:36:04.74 Exhaust.select :close @21:36:04.86 SSV.dial CBV @21:36:04.88 CBV.dial SSV @21:36:05.69 Allowing 130s for 25.0ml of remaining 950.0ml @21:36:05.70 Intake.select :open @21:36:07.87 Intake @32.9psia, vacuum from 20.0 to 24.0psi @21:36:07.89 SS.reconfigure SSinhale.with maxSpeed: 32 @21:36:07.94 SS.seek 25ml @21:37:02.82 Stroke completed -- waiting for pressure to equalize ... @21:37:09.10 Intake.select :close @21:37:09.25 SSV.dial :exhaust @21:37:10.02 Exhaust.select :open @21:37:10.14 SS.reconfigure SSexhale @21:37:10.19 SS.seek 0ml @21:37:19.18 Exhaust.select :close @21:37:19.30 SSV.dial CBV @21:37:19.35 CBV.dial SSV @21:37:20.11 Allowing 130s for 25.0ml of remaining 925.0ml @21:37:20.12 Intake.select :open @21:37:22.29 Intake @33.4psia, vacuum from 20.0 to 24.0psi @21:37:22.31 SS.reconfigure SSinhale.with maxSpeed: 32 @21:37:22.35 SS.seek 25ml @21:38:01.28 Vacuum too high -- waiting up to 40s @21:38:02.57 Intake @32.5psia, vacuum from 20.0 to 24.0psi @21:38:02.58 SS.reconfigure SSinhale.with maxSpeed: 29 @21:38:02.63 SS.seek 25ml @21:38:20.55 Stroke completed -- waiting for pressure to equalize ... @21:38:26.82 Intake.select :close @21:38:26.97 SSV.dial :exhaust @21:38:27.72 Exhaust.select :open @21:38:27.84 SS.reconfigure SSexhale @21:38:27.90 SS.seek 0ml @21:38:36.85 Exhaust.select :close @21:38:36.97 SSV.dial CBV @21:38:36.99 CBV.dial SSV @21:38:37.78 Allowing 130s for 25.0ml of remaining 900.0ml @21:38:37.79 Intake.select :open @21:38:39.96 Intake @33.2psia, vacuum from 20.0 to 24.0psi @21:38:39.98 SS.reconfigure SSinhale.with maxSpeed: 29 @21:38:40.02 SS.seek 25ml @21:39:40.71 Stroke completed -- waiting for pressure to equalize ... @21:39:46.88 Intake.select :close @21:39:47.04 SSV.dial :exhaust @21:39:47.80 Exhaust.select :open @21:39:47.91 SS.reconfigure SSexhale @21:39:47.96 SS.seek 0ml @21:39:56.69 Exhaust.select :close @21:39:56.81 SSV.dial CBV @21:39:56.83 CBV.dial SSV @21:39:57.64 Allowing 130s for 25.0ml of remaining 875.0ml @21:39:57.65 Intake.select :open @21:39:59.82 Intake @33.4psia, vacuum from 20.0 to 24.0psi @21:39:59.84 SS.reconfigure SSinhale.with maxSpeed: 29 @21:39:59.89 SS.seek 25ml @21:41:00.78 Stroke completed -- waiting for pressure to equalize ... @21:41:07.07 Intake.select :close @21:41:07.21 SSV.dial :exhaust @21:41:08.00 Exhaust.select :open @21:41:08.11 SS.reconfigure SSexhale @21:41:08.16 SS.seek 0ml @21:41:17.07 Exhaust.select :close @21:41:17.19 SSV.dial CBV @21:41:17.21 CBV.dial SSV @21:41:18.02 Allowing 130s for 25.0ml of remaining 850.0ml @21:41:18.03 Intake.select :open @21:41:20.20 Intake @33.1psia, vacuum from 20.0 to 24.0psi @21:41:20.22 SS.reconfigure SSinhale.with maxSpeed: 29 @21:41:20.26 SS.seek 25ml @21:42:20.82 Stroke completed -- waiting for pressure to equalize ... @21:42:27.70 Intake.select :close @21:42:27.85 SSV.dial :exhaust @21:42:28.62 Exhaust.select :open @21:42:28.74 SS.reconfigure SSexhale @21:42:28.78 SS.seek 0ml @21:42:38.02 Exhaust.select :close @21:42:38.14 SSV.dial CBV @21:42:38.16 CBV.dial SSV @21:42:38.96 Allowing 130s for 25.0ml of remaining 825.0ml @21:42:38.98 Intake.select :open @21:42:41.15 Intake @33.2psia, vacuum from 20.0 to 24.0psi @21:42:41.17 SS.reconfigure SSinhale.with maxSpeed: 29 @21:42:41.22 SS.seek 25ml @21:43:23.89 GoodNews.email "At 20:43:23 began in tube 2: wcrPhase2\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:129\n{1 => 12, 2 => 10}\nCan@20:43:23, 16.4C, 79% humidity, 14.6psia, 14.252V, 0.840A, 0.840A avg, 11.97W",Subject: "Started TotaldeploymentFINAL3 phase wcrPhase2" @21:43:23.92 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @21:43:23.93 Retry #7 of 10 after 10:00 @21:43:41.88 Stroke completed -- waiting for pressure to equalize ... @21:43:49.28 Intake.select :close @21:43:49.43 SSV.dial :exhaust @21:43:50.20 Exhaust.select :open @21:43:50.31 SS.reconfigure SSexhale @21:43:50.41 SS.seek 0ml @21:43:59.44 Exhaust.select :close @21:43:59.56 SSV.dial CBV @21:43:59.59 CBV.dial SSV @21:44:00.37 Allowing 130s for 25.0ml of remaining 800.0ml @21:44:00.38 Intake.select :open @21:44:02.55 Intake @33.0psia, vacuum from 20.0 to 24.0psi @21:44:02.57 SS.reconfigure SSinhale.with maxSpeed: 29 @21:44:02.62 SS.seek 25ml @21:45:03.68 Stroke completed -- waiting for pressure to equalize ... @21:45:11.24 Intake.select :close @21:45:11.43 SSV.dial :exhaust @21:45:12.20 Exhaust.select :open @21:45:12.31 SS.reconfigure SSexhale @21:45:12.37 SS.seek 0ml @21:45:21.34 Exhaust.select :close @21:45:21.50 SSV.dial CBV @21:45:21.56 CBV.dial SSV @21:45:22.39 Allowing 130s for 25.0ml of remaining 775.0ml @21:45:22.40 Intake.select :open @21:45:24.57 Intake @33.4psia, vacuum from 20.0 to 24.0psi @21:45:24.59 SS.reconfigure SSinhale.with maxSpeed: 29 @21:45:24.64 SS.seek 25ml @21:46:11.59 Vacuum too high -- waiting up to 40s @21:46:13.13 Intake @32.9psia, vacuum from 20.0 to 24.0psi @21:46:13.15 SS.reconfigure SSinhale.with maxSpeed: 26 @21:46:13.20 SS.seek 25ml @21:46:28.80 Stroke completed -- waiting for pressure to equalize ... @21:46:36.20 Intake.select :close @21:46:36.36 SSV.dial :exhaust @21:46:37.14 Exhaust.select :open @21:46:37.26 SS.reconfigure SSexhale @21:46:37.30 SS.seek 0ml @21:46:46.18 Exhaust.select :close @21:46:46.30 SSV.dial CBV @21:46:46.33 CBV.dial SSV @21:46:47.14 Allowing 130s for 25.0ml of remaining 750.0ml @21:46:47.15 Intake.select :open @21:46:49.32 Intake @33.4psia, vacuum from 20.0 to 24.0psi @21:46:49.34 SS.reconfigure SSinhale.with maxSpeed: 26 @21:46:49.39 SS.seek 25ml @21:47:57.18 Stroke completed -- waiting for pressure to equalize ... @21:48:04.70 Intake.select :close @21:48:04.85 SSV.dial :exhaust @21:48:05.64 Exhaust.select :open @21:48:05.75 SS.reconfigure SSexhale @21:48:05.80 SS.seek 0ml @21:48:14.56 Exhaust.select :close @21:48:14.67 SSV.dial CBV @21:48:14.70 CBV.dial SSV @21:48:15.51 Allowing 130s for 25.0ml of remaining 725.0ml @21:48:15.52 Intake.select :open @21:48:17.69 Intake @33.3psia, vacuum from 20.0 to 24.0psi @21:48:17.71 SS.reconfigure SSinhale.with maxSpeed: 26 @21:48:17.75 SS.seek 25ml @21:49:25.57 Stroke completed -- waiting for pressure to equalize ... @21:49:33.10 Intake.select :close @21:49:33.24 SSV.dial :exhaust @21:49:34.03 Exhaust.select :open @21:49:34.14 SS.reconfigure SSexhale @21:49:34.19 SS.seek 0ml @21:49:43.19 Exhaust.select :close @21:49:43.31 SSV.dial CBV @21:49:43.33 CBV.dial SSV @21:49:44.14 Allowing 130s for 25.0ml of remaining 700.0ml @21:49:44.15 Intake.select :open @21:49:46.32 Intake @33.3psia, vacuum from 20.0 to 24.0psi @21:49:46.34 SS.reconfigure SSinhale.with maxSpeed: 26 @21:49:46.38 SS.seek 25ml @21:50:53.88 Stroke completed -- waiting for pressure to equalize ... @21:51:01.90 Intake.select :close @21:51:02.06 SSV.dial :exhaust @21:51:02.83 Exhaust.select :open @21:51:02.94 SS.reconfigure SSexhale @21:51:03.00 SS.seek 0ml @21:51:12.21 Exhaust.select :close @21:51:12.33 SSV.dial CBV @21:51:12.35 CBV.dial SSV @21:51:13.15 Allowing 130s for 25.0ml of remaining 675.0ml @21:51:13.16 Intake.select :open @21:51:15.33 Intake @33.4psia, vacuum from 20.0 to 24.0psi @21:51:15.35 SS.reconfigure SSinhale.with maxSpeed: 26 @21:51:15.40 SS.seek 25ml @21:52:22.99 Stroke completed -- waiting for pressure to equalize ... @21:52:31.75 Intake.select :close @21:52:31.91 SSV.dial :exhaust @21:52:32.69 Exhaust.select :open @21:52:32.80 SS.reconfigure SSexhale @21:52:32.86 SS.seek 0ml @21:52:42.05 Exhaust.select :close @21:52:42.17 SSV.dial CBV @21:52:42.19 CBV.dial SSV @21:52:43.00 Allowing 130s for 25.0ml of remaining 650.0ml @21:52:43.01 Intake.select :open @21:52:45.18 Intake @33.3psia, vacuum from 20.0 to 24.0psi @21:52:45.20 SS.reconfigure SSinhale.with maxSpeed: 26 @21:52:45.24 SS.seek 25ml @21:53:23.96 GoodNews.email "At 20:43:23 began in tube 2: wcrPhase2\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:129\n{1 => 12, 2 => 10}\nCan@20:43:23, 16.4C, 79% humidity, 14.6psia, 14.252V, 0.840A, 0.840A avg, 11.97W",Subject: "Started TotaldeploymentFINAL3 phase wcrPhase2" @21:53:23.99 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @21:53:24.00 Retry #8 of 10 after 10:00 @21:53:45.68 Vacuum too high -- waiting up to 40s @21:53:47.45 SS.reconfigure SSinhale.with maxSpeed: 23 @21:53:47.50 SS.seek 25ml @21:53:55.66 Stroke completed -- waiting for pressure to equalize ... @21:54:03.07 Intake.select :close @21:54:03.23 SSV.dial :exhaust @21:54:04.00 Exhaust.select :open @21:54:04.11 SS.reconfigure SSexhale @21:54:04.16 SS.seek 0ml @21:54:13.11 Exhaust.select :close @21:54:13.23 SSV.dial CBV @21:54:13.25 CBV.dial SSV @21:54:14.06 Allowing 130s for 25.0ml of remaining 625.0ml @21:54:14.07 Intake.select :open @21:54:16.24 Intake @33.1psia, vacuum from 20.0 to 24.0psi @21:54:16.26 SS.reconfigure SSinhale.with maxSpeed: 23 @21:54:16.31 SS.seek 25ml @21:55:32.53 Stroke completed -- waiting for pressure to equalize ... @21:55:40.03 Intake.select :close @21:55:40.19 SSV.dial :exhaust @21:55:40.97 Exhaust.select :open @21:55:41.08 SS.reconfigure SSexhale @21:55:41.14 SS.seek 0ml @21:55:50.16 Exhaust.select :close @21:55:50.28 SSV.dial CBV @21:55:50.30 CBV.dial SSV @21:55:51.11 Allowing 130s for 25.0ml of remaining 600.0ml @21:55:51.12 Intake.select :open @21:55:53.29 Intake @33.2psia, vacuum from 20.0 to 24.0psi @21:55:53.31 SS.reconfigure SSinhale.with maxSpeed: 23 @21:55:53.35 SS.seek 25ml @21:57:09.52 Stroke completed -- waiting for pressure to equalize ... @21:57:18.27 Intake.select :close @21:57:18.42 SSV.dial :exhaust @21:57:19.18 Exhaust.select :open @21:57:19.33 SS.reconfigure SSexhale @21:57:19.39 SS.seek 0ml @21:57:28.56 Exhaust.select :close @21:57:28.67 SSV.dial CBV @21:57:28.69 CBV.dial SSV @21:57:29.48 Allowing 130s for 25.0ml of remaining 575.0ml @21:57:29.49 Intake.select :open @21:57:31.66 Intake @33.2psia, vacuum from 20.0 to 24.0psi @21:57:31.68 SS.reconfigure SSinhale.with maxSpeed: 23 @21:57:31.73 SS.seek 25ml @21:58:48.11 Stroke completed -- waiting for pressure to equalize ... @21:58:58.37 Intake.select :close @21:58:58.55 SSV.dial :exhaust @21:58:59.31 Exhaust.select :open @21:58:59.42 SS.reconfigure SSexhale @21:58:59.47 SS.seek 0ml @21:59:08.48 Exhaust.select :close @21:59:08.59 SSV.dial CBV @21:59:08.62 CBV.dial SSV @21:59:09.40 Allowing 130s for 25.0ml of remaining 550.0ml @21:59:09.41 Intake.select :open @21:59:11.58 Intake @33.3psia, vacuum from 20.0 to 24.0psi @21:59:11.60 SS.reconfigure SSinhale.with maxSpeed: 23 @21:59:11.64 SS.seek 25ml @22:00:27.81 Stroke completed -- waiting for pressure to equalize ... @22:00:39.17 Intake.select :close @22:00:39.33 SSV.dial :exhaust @22:00:40.09 Exhaust.select :open @22:00:40.20 SS.reconfigure SSexhale @22:00:40.25 SS.seek 0ml @22:00:49.22 Exhaust.select :close @22:00:49.34 SSV.dial CBV @22:00:49.39 CBV.dial SSV @22:00:50.15 Allowing 130s for 25.0ml of remaining 525.0ml @22:00:50.16 Intake.select :open @22:00:52.33 Intake @33.5psia, vacuum from 20.0 to 24.0psi @22:00:52.35 SS.reconfigure SSinhale.with maxSpeed: 23 @22:00:52.39 SS.seek 25ml @22:01:47.05 Vacuum too high -- waiting up to 40s @22:01:48.83 Intake @33.3psia, vacuum from 20.0 to 24.0psi @22:01:48.85 SS.reconfigure SSinhale.with maxSpeed: 21 @22:01:48.90 SS.seek 25ml @22:02:13.05 Stroke completed -- waiting for pressure to equalize ... @22:02:24.91 Intake.select :close @22:02:25.07 SSV.dial :exhaust @22:02:25.83 Exhaust.select :open @22:02:25.95 SS.reconfigure SSexhale @22:02:26.00 SS.seek 0ml @22:02:35.02 Exhaust.select :close @22:02:35.14 SSV.dial CBV @22:02:35.16 CBV.dial SSV @22:02:35.97 Allowing 130s for 25.0ml of remaining 500.0ml @22:02:35.98 Intake.select :open @22:02:38.15 Intake @33.4psia, vacuum from 20.0 to 24.0psi @22:02:38.17 SS.reconfigure SSinhale.with maxSpeed: 21 @22:02:38.22 SS.seek 25ml @22:03:24.03 GoodNews.email "At 20:43:23 began in tube 2: wcrPhase2\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:129\n{1 => 12, 2 => 10}\nCan@20:43:23, 16.4C, 79% humidity, 14.6psia, 14.252V, 0.840A, 0.840A avg, 11.97W",Subject: "Started TotaldeploymentFINAL3 phase wcrPhase2" @22:03:24.06 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @22:03:24.07 Retry #9 of 10 after 10:00 @22:04:01.61 Stroke completed -- waiting for pressure to equalize ... @22:04:14.08 Intake.select :close @22:04:14.23 SSV.dial :exhaust @22:04:15.01 Exhaust.select :open @22:04:15.12 SS.reconfigure SSexhale @22:04:15.17 SS.seek 0ml @22:04:24.13 Exhaust.select :close @22:04:24.25 SSV.dial CBV @22:04:24.27 CBV.dial SSV @22:04:25.59 Allowing 130s for 25.0ml of remaining 475.0ml @22:04:25.60 Intake.select :open @22:04:27.77 Intake @33.3psia, vacuum from 20.0 to 24.0psi @22:04:27.79 SS.reconfigure SSinhale.with maxSpeed: 21 @22:04:27.84 SS.seek 25ml @22:05:51.77 Stroke completed -- waiting for pressure to equalize ... @22:06:04.92 Intake.select :close @22:06:05.07 SSV.dial :exhaust @22:06:05.86 Exhaust.select :open @22:06:05.97 SS.reconfigure SSexhale @22:06:06.02 SS.seek 0ml @22:06:15.11 Exhaust.select :close @22:06:15.23 SSV.dial CBV @22:06:15.28 CBV.dial SSV @22:06:16.04 Allowing 130s for 25.0ml of remaining 450.0ml @22:06:16.05 Intake.select :open @22:06:18.22 Intake @33.3psia, vacuum from 20.0 to 24.0psi @22:06:18.24 SS.reconfigure SSinhale.with maxSpeed: 21 @22:06:18.29 SS.seek 25ml @22:07:27.96 Vacuum too high -- waiting up to 40s @22:07:29.99 Intake @33.3psia, vacuum from 20.0 to 24.0psi @22:07:30.01 SS.reconfigure SSinhale.with maxSpeed: 19 @22:07:30.05 SS.seek 25ml @22:07:45.88 Stroke completed -- waiting for pressure to equalize ... @22:08:00.18 Intake.select :close @22:08:00.33 SSV.dial :exhaust @22:08:01.11 Exhaust.select :open @22:08:01.22 SS.reconfigure SSexhale @22:08:01.27 SS.seek 0ml @22:08:09.97 Exhaust.select :close @22:08:10.09 SSV.dial CBV @22:08:10.11 CBV.dial SSV @22:08:10.93 Allowing 130s for 25.0ml of remaining 425.0ml @22:08:10.94 Intake.select :open @22:08:13.11 Intake @33.4psia, vacuum from 20.0 to 24.0psi @22:08:13.13 SS.reconfigure SSinhale.with maxSpeed: 19 @22:08:13.18 SS.seek 25ml @22:09:45.67 Stroke completed -- waiting for pressure to equalize ... @22:10:01.27 Intake.select :close @22:10:01.42 SSV.dial :exhaust @22:10:02.20 Exhaust.select :open @22:10:02.31 SS.reconfigure SSexhale @22:10:02.36 SS.seek 0ml @22:10:11.31 Exhaust.select :close @22:10:11.43 SSV.dial CBV @22:10:11.45 CBV.dial SSV @22:10:12.26 Allowing 130s for 25.0ml of remaining 400.0ml @22:10:12.27 Intake.select :open @22:10:14.44 Intake @33.3psia, vacuum from 20.0 to 24.0psi @22:10:14.46 SS.reconfigure SSinhale.with maxSpeed: 19 @22:10:14.50 SS.seek 25ml @22:11:46.75 Stroke completed -- waiting for pressure to equalize ... @22:12:05.95 Intake.select :close @22:12:06.11 SSV.dial :exhaust @22:12:06.89 Exhaust.select :open @22:12:07.00 SS.reconfigure SSexhale @22:12:07.06 SS.seek 0ml @22:12:15.83 Exhaust.select :close @22:12:15.95 SSV.dial CBV @22:12:15.97 CBV.dial SSV @22:12:16.78 Allowing 130s for 25.0ml of remaining 375.0ml @22:12:16.79 Intake.select :open @22:12:18.96 Intake @33.4psia, vacuum from 20.0 to 24.0psi @22:12:18.98 SS.reconfigure SSinhale.with maxSpeed: 19 @22:12:19.02 SS.seek 25ml @22:13:17.65 Vacuum too high -- waiting up to 40s @22:13:19.44 Intake @33.1psia, vacuum from 20.0 to 24.0psi @22:13:19.46 SS.reconfigure SSinhale.with maxSpeed: 17 @22:13:19.51 SS.seek 25ml @22:13:24.09 GoodNews.email "At 20:43:23 began in tube 2: wcrPhase2\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:129\n{1 => 12, 2 => 10}\nCan@20:43:23, 16.4C, 79% humidity, 14.6psia, 14.252V, 0.840A, 0.840A avg, 11.97W",Subject: "Started TotaldeploymentFINAL3 phase wcrPhase2" @22:13:24.12 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @22:13:24.13 Retry #10 of 10 after 10:00 @22:13:58.06 Stroke completed -- waiting for pressure to equalize ... @22:14:16.75 Intake.select :close @22:14:16.91 SSV.dial :exhaust @22:14:17.68 Exhaust.select :open @22:14:17.79 SS.reconfigure SSexhale @22:14:17.85 SS.seek 0ml @22:14:26.63 Exhaust.select :close @22:14:26.75 SSV.dial CBV @22:14:26.78 CBV.dial SSV @22:14:27.59 Allowing 130s for 25.0ml of remaining 350.0ml @22:14:27.60 Intake.select :open @22:14:29.77 Intake @33.4psia, vacuum from 20.0 to 24.0psi @22:14:29.79 SS.reconfigure SSinhale.with maxSpeed: 17 @22:14:29.83 SS.seek 25ml @22:16:13.20 Stroke completed -- waiting for pressure to equalize ... @22:16:34.26 Intake.select :close @22:16:34.41 SSV.dial :exhaust @22:16:35.20 Exhaust.select :open @22:16:35.32 SS.reconfigure SSexhale @22:16:35.36 SS.seek 0ml @22:16:44.41 Exhaust.select :close @22:16:44.53 SSV.dial CBV @22:16:44.56 CBV.dial SSV @22:16:45.37 Allowing 130s for 25.0ml of remaining 325.0ml @22:16:45.38 Intake.select :open @22:16:47.55 Intake @33.1psia, vacuum from 20.0 to 24.0psi @22:16:47.57 SS.reconfigure SSinhale.with maxSpeed: 17 @22:16:47.62 SS.seek 25ml @22:18:30.75 Stroke completed -- waiting for pressure to equalize ... @22:18:55.39 SS.reconfigure SSconfig @22:18:55.45 SS.hold @22:18:55.59 Intake.select :close @22:18:55.74 ShallowSampler::Clogged in WCR -- progress too slow after 1700.0ml @22:18:55.75 SSV.dial :exhaust @22:18:56.53 Exhaust.select :open @22:18:56.64 SS.reconfigure SSexhale @22:18:56.69 SS.seek 0ml @22:19:05.75 Exhaust.select :close @22:19:05.87 Sampled 1700.0ml @22:19:05.89 Drying puck before fixing for 4:00 @22:19:05.90 CBV.dial :waste2b @22:19:06.67 delay 1:00 @22:20:06.70 CSV.dial :air @22:20:07.95 CS.seek 10ml @22:20:30.87 Pumping wcr puck from tube 2 up to 55psi... @22:20:30.88 CBV.dial SSV @22:20:32.22 CBV.dial between CSV and waste2b @22:20:33.21 CSV.dial between air and CTV @22:20:33.52 CS.seek 6.862ml @22:20:41.65 CTV.dial CSV,avoiding: :air @22:20:41.67 CSV.dial CTV @22:20:42.50 CS.reconfigure CSpressurize @22:20:42.55 CS.seek 0ml @22:21:00.75 CSV.dial :air @22:21:01.23 CS.reconfigure CSfast @22:21:01.29 CS.seek 10ml @22:21:24.28 CSV.dial between air and CTV @22:21:24.63 CS.seek 2.376ml @22:21:42.34 CTV.dial CSV,avoiding: :air @22:21:42.37 CSV.dial CTV @22:21:42.67 CS.reconfigure CSpressurize @22:21:42.73 CS.seek 0ml @22:21:45.82 CBV.dial :waste2b @22:21:46.17 CS.reconfigure CSconfig @22:21:46.22 CS.seek :empty @22:21:50.75 delay 1:00 @22:22:50.78 CTV.dial :air,avoiding: :intake @22:22:52.08 Fix #1 of 2 (with rnal) for 2:00 @22:22:52.10 CSV.dial CSR @22:22:52.61 Pulling 2ml of CSR.rnal @22:22:52.62 CSR.select :rnal @22:22:52.74 CS.seek 2ml @22:22:58.91 delay 5 seconds @22:23:03.94 CSR.select :air @22:23:04.05 CS.reconfigure CSfast @22:23:04.10 CS.seek 10ml @22:23:22.50 Pumping wcr puck from tube 2 up to 55psi... @22:23:22.51 CBV.dial SSV @22:23:23.82 CBV.dial between CSV and waste2b @22:23:24.15 GoodNews.email "At 20:43:23 began in tube 2: wcrPhase2\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:129\n{1 => 12, 2 => 10}\nCan@20:43:23, 16.4C, 79% humidity, 14.6psia, 14.252V, 0.840A, 0.840A avg, 11.97W",Subject: "Started TotaldeploymentFINAL3 phase wcrPhase2" @22:23:24.18 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @22:23:24.19 BACfans.email "Started stand-alone bac WCR in tube 2 at 20:47:34CEST12-Sep-19\n{1 => 12, 2 => 10}\nCan@20:44:37, 16.4C, 79% humidity, 14.6psia, 14.272V, 0.746A, 0.739A avg, 10.64W",Subject: "bac wcr sampling at most 2000ml" @22:23:24.22 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @22:23:24.23 Retry #1 of 10 after 10:00 @22:23:24.79 CSV.dial between air and CTV @22:23:25.42 CS.seek 6.816ml @22:23:33.41 CTV.dial CSV,avoiding: :air @22:23:33.44 CSV.dial CTV @22:23:34.81 CS.reconfigure CSpressurize @22:23:34.87 CS.seek 0ml @22:23:52.68 delay 2:00 @22:25:52.71 CBV.dial :waste2b @22:25:53.07 CS.reconfigure CSconfig @22:25:53.12 CS.seek :empty @22:25:53.63 delay 1:00 @22:26:53.66 CTV.dial :air,avoiding: :intake @22:26:54.97 Fix #2 of 2 (with rnal) for 2:00 @22:26:54.99 CSV.dial CSR @22:26:55.50 Pulling 2ml of CSR.rnal @22:26:55.51 CSR.select :rnal @22:26:56.17 CS.seek 2ml @22:27:02.28 delay 5 seconds @22:27:07.31 CSR.select :air @22:27:07.43 CS.reconfigure CSfast @22:27:07.47 CS.seek 10ml @22:27:25.75 Pumping wcr puck from tube 2 up to 55psi... @22:27:25.76 CBV.dial SSV @22:27:27.08 CBV.dial between CSV and waste2b @22:27:28.06 CSV.dial between air and CTV @22:27:28.81 CS.seek 6.823ml @22:27:36.72 CTV.dial CSV,avoiding: :air @22:27:36.75 CSV.dial CTV @22:27:38.10 CS.reconfigure CSpressurize @22:27:38.16 CS.seek 0ml @22:27:54.74 delay 2:00 @22:29:54.77 CBV.dial :waste2b @22:29:55.11 CS.reconfigure CSconfig @22:29:55.16 CS.seek :empty @22:29:57.54 delay 1:00 @22:30:57.57 CTV.dial :air,avoiding: :intake @22:30:58.94 Storing wcr puck in Collection Clamp to tube 1 @22:30:59.12 SC.reconfigure SCtight @22:30:59.17 SC.seek 1 @22:31:01.32 SC.reconfigure SCloose @22:31:01.54 Elbow.reconfigure TightElbow @22:31:01.59 Elbow.seek 1 @22:31:03.49 Forearm.reconfigure TightForearm @22:31:03.54 Forearm.seek 1 + 700 counts @22:31:04.56 SE.reconfigure SEpick @22:31:04.64 SE.seek beamBreak @22:31:06.59 SE.configure SEconfig @22:31:06.76 Elbow.reconfigure LimpElbow @22:31:06.81 Forearm.reconfigure LooseForearm @22:31:06.86 Forearm.seek 1 @22:31:07.14 Elbow.hold @22:31:07.21 Elbow.reconfigure TightElbow @22:31:07.31 SE.seek 11.07 Pucks below Top Plate @22:31:07.74 Forearm.force -20 @22:31:07.78 Forearm.seek 1 + 344 counts @22:31:08.10 Forearm.reconfigure TightForearm @22:31:08.15 Forearm.seek 1 + 700 counts @22:31:08.40 SE.seek 12.07 Pucks below Top Plate @22:31:09.04 Forearm.reconfigure LooseForearm @22:31:09.09 Forearm.seek :retract @22:31:10.02 Elbow.seek CC @22:31:11.61 Elbow.reconfigure LooseElbow @22:31:11.71 Forearm.seek CC @22:31:12.78 Hand.close @22:31:13.83 CC.seek :open @22:31:18.36 Forearm.seek :retract @22:31:19.46 Elbow.reconfigure TightElbow @22:31:19.51 Elbow.seek 1 @22:31:21.41 Elbow.reconfigure LimpElbow @22:31:21.47 Forearm.seek 1 @22:31:22.47 Elbow.hold @22:31:22.54 Elbow.reconfigure TightElbow @22:31:22.59 Hand.open @22:31:23.34 Forearm.force -20 @22:31:23.38 Forearm.seek 1 + 347 counts @22:31:23.68 Forearm.reconfigure TightForearm @22:31:23.73 Forearm.seek 1 + 700 counts @22:31:24.12 SE.seek :down @22:31:25.26 Forearm.reconfigure LooseForearm @22:31:25.31 Forearm.seek :retract @22:31:25.74 SE.coast @22:31:26.30 SC.coast @22:31:26.34 Starting finalPurge @22:31:26.35 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @22:31:26.43 Hand.open @22:31:26.84 Elbow.seek FlushPuck::Garage @22:31:28.75 Elbow.reconfigure LooseElbow @22:31:28.85 Forearm.seek :garage @22:31:29.82 Hand.close @22:31:30.59 Forearm.seek :retract @22:31:31.60 Elbow.reconfigure TightElbow @22:31:31.64 Elbow.seek CC @22:31:34.52 Elbow.reconfigure LooseElbow @22:31:34.62 Forearm.reconfigure TightForearm @22:31:34.66 Forearm.seek CC @22:31:36.00 CC.seek :closed @22:31:40.24 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.76 @22:31:40.32 Hand.open @22:31:41.03 Forearm.reconfigure LooseForearm @22:31:41.08 Forearm.seek :retract @22:31:42.07 Flushing the Collection Valve::Manifold @22:31:42.20 CSV.dial CSR @22:31:43.21 Pulling 5ml of CSR.flush @22:31:43.22 CSR.select :flush @22:31:43.34 CS.seek 5ml @22:31:57.04 delay 5 seconds @22:32:02.07 CSR.select :air @22:32:02.23 CS.reconfigure CSfast @22:32:02.28 CS.seek 7.5ml @22:32:08.85 CSV.dial :waste @22:32:10.13 CS.reconfigure CSconfig @22:32:10.18 CS.seek 0ml @22:32:30.35 Flushing lower manifold's waste1 port @22:32:30.48 CSV.dial :flush @22:32:30.93 CS.reconfigure CSfast @22:32:30.98 CS.seek 5ml @22:32:42.82 delay 5 seconds @22:32:47.85 CSV.dial :air @22:32:48.37 CS.seek 7ml @22:32:53.82 CSV.dial CTV @22:32:53.87
CTV.dial CSV @22:32:55.24 CBV.dial :waste1 @22:32:56.02 CS.seek 0ml @22:33:12.10 Flushing lower manifold's waste2b port @22:33:12.23 CSV.dial :flush @22:33:12.97 CS.seek 5ml @22:33:24.26 BACfans.email "Started stand-alone bac WCR in tube 2 at 20:47:34CEST12-Sep-19\n{1 => 12, 2 => 10}\nCan@20:44:37, 16.4C, 79% humidity, 14.6psia, 14.272V, 0.746A, 0.739A avg, 10.64W",Subject: "bac wcr sampling at most 2000ml" @22:33:24.29 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @22:33:24.30 Retry #2 of 10 after 10:00 @22:33:25.01
delay 5 seconds @22:33:30.04 CSV.dial :air @22:33:30.56 CS.seek 7ml @22:33:35.95 CSV.dial CTV @22:33:35.97
CTV.dial CSV @22:33:36.43 CBV.dial :waste2b @22:33:37.20 CS.seek 0ml @22:33:53.26 Flushing upper manifold @22:33:53.39 CSV.dial :flush @22:33:54.14 CS.seek 5ml @22:34:06.20 delay 5 seconds @22:34:11.22 CSV.dial :air @22:34:11.77 CS.seek 7ml @22:34:17.23 CSV.dial CBV @22:34:17.25
CBV.dial CSV @22:34:18.37 CTV.dial :air @22:34:19.68 CS.seek 0ml @22:34:35.75 delay 7 seconds @22:34:42.79 CS.seek 3ml @22:34:50.41 CSV.dial :waste @22:34:50.88 CS.seek 0ml @22:34:58.46 CSV.dial :flush @22:34:58.92 CS.seek 3ml @22:35:06.63 delay 5 seconds @22:35:11.65 CSV.dial :air @22:35:12.19 CS.seek 5.001ml @22:35:17.55 CSV.dial :air @22:35:17.57 CS.seek 0ml @22:35:29.35 delay 7 seconds @22:35:36.39 CS.seek 3ml @22:35:44.00 CSV.dial :waste @22:35:44.75 CS.seek 0ml @22:35:52.23 Disinfecting external Intake with mfbkill @22:35:52.36 CSV.dial CSR @22:35:53.71 Pulling 8ml of CSR.mfbkill @22:35:53.72 CSR.select :mfbkill @22:35:53.84 CS.seek 8ml @22:36:12.32 delay 5 seconds @22:36:17.35 CSR.select :air @22:36:17.48 CS.seek 10ml @22:36:22.85 CSV.dial CBV @22:36:22.87
CBV.dial CSV @22:36:23.89 CTV.dial :intake @22:36:24.65 Intake.select :open @22:36:24.76 CS.seek 2ml @22:36:44.72 Intake.select :close @22:36:44.83 CTV.dial :air @22:36:45.60 CS.reconfigure CSconfig @22:36:45.65 CS.seek 0ml @22:36:51.76 Flushing lower manifold's waste1 port @22:36:51.77 CSV.dial CSR @22:36:52.83 Pulling 4ml of CSR.mfbkill @22:36:52.84 CSR.select :mfbkill @22:36:52.96 CS.seek 4ml @22:37:04.14 delay 5 seconds @22:37:09.17 CSR.select :air @22:37:09.28 CS.reconfigure CSfast @22:37:09.34 CS.seek 10ml @22:37:23.39 CSV.dial CTV @22:37:23.42
CTV.dial CSV @22:37:24.82 CBV.dial :waste1 @22:37:25.29 CS.seek 0ml @22:37:48.23 Flushing lower manifold's waste2b port @22:37:48.24 CSV.dial CSR @22:37:48.76 Pulling 4ml of CSR.mfbkill @22:37:48.77 CSR.select :mfbkill @22:37:48.89 CS.reconfigure CSconfig @22:37:48.93 CS.seek 4ml @22:38:00.14 delay 5 seconds @22:38:05.17 CSR.select :air @22:38:05.28 CS.reconfigure CSfast @22:38:05.33 CS.seek 10ml @22:38:19.32 CSV.dial CTV @22:38:19.34
CTV.dial CSV @22:38:19.82 CBV.dial :waste2b @22:38:20.59 CS.seek 0ml @22:38:43.17 Killing upper manifold @22:38:43.18 CSV.dial CSR @22:38:43.70 Pulling 4ml of CSR.mfbkill @22:38:43.71 CSR.select :mfbkill @22:38:43.83 CS.reconfigure CSconfig @22:38:43.87 CS.seek 4ml @22:38:55.04 delay 5 seconds @22:39:00.07 CSR.select :air @22:39:00.18 CS.reconfigure CSfast @22:39:00.23 CS.seek 10ml @22:39:14.20 CSV.dial CBV @22:39:14.22
CBV.dial CSV @22:39:15.29 CTV.dial :air @22:39:16.61 CS.seek 0ml @22:39:39.14 delay 7 seconds @22:39:46.17 CS.seek 3ml @22:39:54.03 CSV.dial :waste @22:39:54.51 CS.seek 0ml @22:40:02.11 Flushing upper manifold @22:40:02.26 CSV.dial :flush @22:40:02.76 CS.seek 5ml @22:40:14.64 delay 5 seconds @22:40:19.66 CSV.dial :air @22:40:20.33 CS.seek 7ml @22:40:25.70 CSV.dial CBV @22:40:25.72
CBV.dial CSV @22:40:26.75 CTV.dial :air @22:40:26.77 CS.seek 0ml @22:40:42.95 delay 7 seconds @22:40:49.99 CS.seek 3ml @22:40:57.57 CSV.dial :waste @22:40:58.03 CS.seek 0ml @22:41:05.69 CSV.dial :flush @22:41:06.15 CS.seek 3ml @22:41:13.73 delay 5 seconds @22:41:18.75 CSV.dial :air @22:41:19.27 CS.seek 5ml @22:41:24.63 CSV.dial :air @22:41:24.66 CS.seek 0ml @22:41:36.38 delay 7 seconds @22:41:43.42 CS.seek 3ml @22:41:50.94 CSV.dial :waste @22:41:51.70 CS.seek 0ml @22:41:59.30 Flushing lower manifold's waste1 port @22:41:59.42 CSV.dial :flush @22:42:00.38 CS.seek 5ml @22:42:12.30 delay 5 seconds @22:42:17.32 CSV.dial :air @22:42:17.85 CS.seek 7ml @22:42:23.26 CSV.dial CTV @22:42:23.28
CTV.dial CSV @22:42:24.67 CBV.dial :waste1 @22:42:25.15 CS.seek 0ml @22:42:41.36 Flushing lower manifold's waste2b port @22:42:41.49 CSV.dial :flush @22:42:42.26 CS.seek 5ml @22:42:54.20 delay 5 seconds @22:42:59.22 CSV.dial :air @22:42:59.74 CS.seek 7ml @22:43:05.23 CSV.dial CTV @22:43:05.25
CTV.dial CSV @22:43:05.70 CBV.dial :waste2b @22:43:06.47 CS.seek 0ml @22:43:22.55 Disinfecting external Intake with kill @22:43:22.68 CSV.dial CSR @22:43:23.18 Pulling 8ml of CSR.kill @22:43:23.19 CSR.select :kill @22:43:23.31 CS.seek 8ml @22:43:24.33 BACfans.email "Started stand-alone bac WCR in tube 2 at 20:47:34CEST12-Sep-19\n{1 => 12, 2 => 10}\nCan@20:44:37, 16.4C, 79% humidity, 14.6psia, 14.272V, 0.746A, 0.739A avg, 10.64W",Subject: "bac wcr sampling at most 2000ml" @22:43:24.36 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @22:43:24.37 Retry #3 of 10 after 10:00 @22:43:41.55
delay 5 seconds @22:43:46.58 CSR.select :air @22:43:46.70 CS.seek 10ml @22:43:52.06 CSV.dial CBV @22:43:52.08
CBV.dial CSV @22:43:53.15 CTV.dial :intake @22:43:53.90 Intake.select :open @22:43:54.03 CS.seek 2ml @22:44:13.26 Intake.select :close @22:44:13.38 CTV.dial :air @22:44:14.14 CS.reconfigure CSconfig @22:44:14.19 CS.seek 0ml @22:44:20.47 CSV.dial CSR @22:44:21.55 Pulling 5ml of CSR.flush CSR.select :flush @22:44:21.68 CS.seek 5ml @22:44:35.55 delay 5 seconds @22:44:40.57 CSR.select :air @22:44:40.69 CS.seek 10ml @22:44:54.48 CSV.dial CBV @22:44:55.50 CS.seek 0ml @22:45:22.08 Garaging FlushPuck in Collection Clamp @22:45:22.15 Elbow.reconfigure TightElbow @22:45:22.20 Elbow.seek CC @22:45:22.49 Elbow.reconfigure LooseElbow @22:45:22.59 Forearm.seek CC @22:45:23.61 Hand.close @22:45:24.23 CC.seek :open @22:45:28.57 Forearm.seek :retract @22:45:29.60 Elbow.reconfigure TightElbow @22:45:29.65 Elbow.seek :garage @22:45:32.65 Elbow.reconfigure LooseElbow @22:45:32.75 Forearm.reconfigure TightForearm @22:45:32.79 Forearm.seek :garage @22:45:33.97 Hand.open @22:45:34.66 Forearm.reconfigure LooseForearm @22:45:34.71 Forearm.seek :retract @22:45:35.75 Completed [wcrPhase2] begun 20:43:23 @22:45:35.77 Preparing to sleep until Fri Sep 13 00:43:17 +0200 2019 @22:45:35.83 SS.coast @22:45:35.86
Asleep til 00:43CEST13-Sep-19 @22:45:35.90 Continuing at 00:43CEST13-Sep-19 @22:45:35.93 CC.coast @22:45:36.04 CS.coast @22:45:36.56 PC.coast @22:45:36.60 Forearm.coast @22:45:36.64 Elbow.coast @22:45:37.03 Gate.power :core,:OFF @22:45:37.04 Gate.power :raw,:OFF @22:45:37.05 Shutdown @22:53:24.40 BACfans.email "Started stand-alone bac WCR in tube 2 at 20:47:34CEST12-Sep-19\n{1 => 12, 2 => 10}\nCan@20:44:37, 16.4C, 79% humidity, 14.6psia, 14.272V, 0.746A, 0.739A avg, 10.64W",Subject: "bac wcr sampling at most 2000ml" @22:53:24.43 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @22:53:24.44 Retry #4 of 10 after 10:00 @23:03:24.47 BACfans.email "Started stand-alone bac WCR in tube 2 at 20:47:34CEST12-Sep-19\n{1 => 12, 2 => 10}\nCan@20:44:37, 16.4C, 79% humidity, 14.6psia, 14.272V, 0.746A, 0.739A avg, 10.64W",Subject: "bac wcr sampling at most 2000ml" @23:03:24.50 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @23:03:24.51 Retry #5 of 10 after 10:00 @23:13:24.54 BACfans.email "Started stand-alone bac WCR in tube 2 at 20:47:34CEST12-Sep-19\n{1 => 12, 2 => 10}\nCan@20:44:37, 16.4C, 79% humidity, 14.6psia, 14.272V, 0.746A, 0.739A avg, 10.64W",Subject: "bac wcr sampling at most 2000ml" @23:13:24.57 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @23:13:24.58 Retry #6 of 10 after 10:00 @23:23:24.61 BACfans.email "Started stand-alone bac WCR in tube 2 at 20:47:34CEST12-Sep-19\n{1 => 12, 2 => 10}\nCan@20:44:37, 16.4C, 79% humidity, 14.6psia, 14.272V, 0.746A, 0.739A avg, 10.64W",Subject: "bac wcr sampling at most 2000ml" @23:23:24.64 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @23:23:24.65 Retry #7 of 10 after 10:00 @23:33:24.68 BACfans.email "Started stand-alone bac WCR in tube 2 at 20:47:34CEST12-Sep-19\n{1 => 12, 2 => 10}\nCan@20:44:37, 16.4C, 79% humidity, 14.6psia, 14.272V, 0.746A, 0.739A avg, 10.64W",Subject: "bac wcr sampling at most 2000ml" @23:33:24.71 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @23:33:24.72 Retry #8 of 10 after 10:00 @23:43:24.75 BACfans.email "Started stand-alone bac WCR in tube 2 at 20:47:34CEST12-Sep-19\n{1 => 12, 2 => 10}\nCan@20:44:37, 16.4C, 79% humidity, 14.6psia, 14.272V, 0.746A, 0.739A avg, 10.64W",Subject: "bac wcr sampling at most 2000ml" @23:43:24.78 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @23:43:24.79 Retry #9 of 10 after 10:00 @23:53:24.82 BACfans.email "Started stand-alone bac WCR in tube 2 at 20:47:34CEST12-Sep-19\n{1 => 12, 2 => 10}\nCan@20:44:37, 16.4C, 79% humidity, 14.6psia, 14.272V, 0.746A, 0.739A avg, 10.64W",Subject: "bac wcr sampling at most 2000ml" @23:53:24.85 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @23:53:24.86 Retry #10 of 10 after 10:00 @00:03:24.89CEST13-Sep-19 BACfans.email "Started stand-alone bac WCR in tube 2 at 20:47:34CEST12-Sep-19\n{1 => 12, 2 => 10}\nCan@20:44:37, 16.4C, 79% humidity, 14.6psia, 14.272V, 0.746A, 0.739A avg, 10.64W",Subject: "bac wcr sampling at most 2000ml" @00:03:24.92 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @00:03:24.93 GoodNews.email "Then, in tube 2: wcrPhase2\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:134\n{1 => 13, 2 => 9}\nCan@22:45:35, 17.0C, 78% humidity, 14.6psia, 14.237V, 0.468A, 0.468A avg, 6.66W",Subject: "Asleep until 00:43CEST13-Sep-19" @00:03:24.96 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @00:03:24.97 Retry #1 of 10 after 10:00 @00:13:25.00 GoodNews.email "Then, in tube 2: wcrPhase2\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:134\n{1 => 13, 2 => 9}\nCan@22:45:35, 17.0C, 78% humidity, 14.6psia, 14.237V, 0.468A, 0.468A avg, 6.66W",Subject: "Asleep until 00:43CEST13-Sep-19" @00:13:25.03 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @00:13:25.04 Retry #2 of 10 after 10:00 @00:23:25.07 GoodNews.email "Then, in tube 2: wcrPhase2\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:134\n{1 => 13, 2 => 9}\nCan@22:45:35, 17.0C, 78% humidity, 14.6psia, 14.237V, 0.468A, 0.468A avg, 6.66W",Subject: "Asleep until 00:43CEST13-Sep-19" @00:23:25.14 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @00:23:25.15 Retry #3 of 10 after 10:00 @00:33:25.18 GoodNews.email "Then, in tube 2: wcrPhase2\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:134\n{1 => 13, 2 => 9}\nCan@22:45:35, 17.0C, 78% humidity, 14.6psia, 14.237V, 0.468A, 0.468A avg, 6.66W",Subject: "Asleep until 00:43CEST13-Sep-19" @00:33:25.21 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @00:33:25.22 Retry #4 of 10 after 10:00 @00:43:17.64
Powering core bus @00:43:22.49 Gate.power :raw,:ON @00:43:23.13 SPE.coast @00:43:23.19 SPE.configure SPEconfig @00:43:23.28 AS.configure ASconfig @00:43:23.38 SS.configure SSconfig @00:43:23.48 CC.configure CCconfig @00:43:23.57 CS.configure CSconfig @00:43:23.66 PC.configure PCconfig @00:43:23.76 PS.configure PSconfig @00:43:23.87 Forearm.configure LooseForearm @00:43:23.96 Elbow.configure LooseElbow @00:43:24.08 SC.configure SCloose @00:43:24.18 SE.configure SEconfig @00:43:24.22 CH.coast @00:43:24.27 CH.configure CHconfig @00:43:24.31 PH.coast @00:43:24.37 PH.configure PHconfig @00:43:24.40 PCR.off @00:43:24.44 PCR.pidtemp= 50,0.1,0,300,25,25 @00:43:24.47 PCR.caltemp= 2.8814,0.0372,0 @00:43:24.49 All dwarf controllers running firmware version 3.75 @00:43:24.60 Valve.reset @00:43:24.76 PCR.off @00:43:24.80 PCR.pidtemp= 50,0.1,0,300,25,25 @00:43:24.83 PCR.caltemp= 2.8814,0.0372,0 @00:43:25.00 Intake.select :close @00:43:25.02 Exhaust.select :close @00:43:25.06 CTV.dial :air @00:43:25.15 PSV.dial :air @00:43:25.19 CSV.dial :waste1 @00:43:25.32
Forearm.reconfigure LooseForearm @00:43:25.37 Forearm.home.seek :retract @00:43:25.39 GoodNews.email "Then, in tube 2: wcrPhase2\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:134\n{1 => 13, 2 => 9}\nCan@22:45:35, 17.0C, 78% humidity, 14.6psia, 14.237V, 0.468A, 0.468A avg, 6.66W",Subject: "Asleep until 00:43CEST13-Sep-19" @00:43:25.46 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @00:43:25.48 Retry #5 of 10 after 10:00 @00:43:25.55 PS.reconfigure PSconfig @00:43:25.60 PS.home.seek 0ml @00:43:25.70 SSV.dial :spit,avoiding: :exhaust @00:43:26.15 Hand.close @00:43:26.21
Elbow.jog 1500 @00:43:26.53 CS.reconfigure CSfast @00:43:26.56 SS.reconfigure SSconfig @00:43:26.65 CS.home.seek 0ml @00:43:26.68 SS.home.seek 0ml @00:43:26.82
Elbow.reconfigure LooseElbow @00:43:26.86 Elbow.home.seek :clear @00:43:30.24 SE.reconfigure SEconfig @00:43:30.28 SE.home.seek :down @00:43:30.95 SC.reconfigure SCsloppy @00:43:30.99 SC.home.seek :initial @00:43:33.20 Forearm.seek :retract @00:43:33.76 Elbow.seek :hammer @00:43:34.66 PS.coast @00:43:35.08
Forearm.reconfigure TightForearm @00:43:35.12 Forearm.seek 5 + 350 counts @00:43:36.21 Forearm.seek :retract @00:43:37.40 PC.reconfigure PCconfig @00:43:37.41
CC.reconfigure CCconfig @00:43:37.51 PC.home.seek :open @00:43:37.52
CC.home.seek :open @00:43:40.96 Phase [wcrPhase2] @00:43:40.97CEST13-Sep-19 ==================> STARTED WCRSphase (phase #4) <=== @00:43:40.99 Starting initialPurge @00:43:41.00 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @00:43:41.07 Hand.open @00:43:41.80 Elbow.reconfigure TightElbow @00:43:41.84 Elbow.seek FlushPuck::Garage @00:43:43.59 Elbow.reconfigure LooseElbow @00:43:43.69 Forearm.reconfigure LooseForearm @00:43:43.73 Forearm.seek :garage @00:43:44.77 Hand.close @00:43:45.66 Forearm.seek :retract @00:43:46.68 Elbow.reconfigure TightElbow @00:43:46.72 Elbow.seek CC @00:43:49.62 Elbow.reconfigure LooseElbow @00:43:49.72 Forearm.reconfigure TightForearm @00:43:49.76 Forearm.seek CC @00:43:51.07 CC.seek :closed @00:43:55.34 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.75 @00:43:55.41 Hand.open @00:43:56.10 Forearm.reconfigure LooseForearm @00:43:56.15 Forearm.seek :retract @00:43:57.15 Flushing the Collection Valve::Manifold @00:43:57.28 CSV.dial CSR @00:43:58.63 Pulling 5ml of CSR.flush @00:43:58.64 CSR.select :flush @00:43:58.76 CS.reconfigure CSconfig @00:43:58.80 CS.seek 5ml @00:44:12.50 delay 5 seconds @00:44:17.53 CSR.select :air @00:44:17.69 CS.reconfigure CSfast @00:44:17.74 CS.seek 7.5ml @00:44:24.30 CSV.dial :waste @00:44:25.59 CS.reconfigure CSconfig @00:44:25.64 CS.seek 0ml @00:44:45.84 Purging external Intake @00:44:45.96 CSV.dial :flush @00:44:46.48 CS.reconfigure CSfast @00:44:46.53 CS.seek 10ml @00:45:09.47 delay 5 seconds @00:45:14.49 CSV.dial CBV @00:45:14.52
CBV.dial CSV @00:45:15.42 CTV.dial :intake @00:45:16.20 Intake.select :open @00:45:16.36 CS.seek 0ml @00:45:40.49 Intake.select :close @00:45:40.61 CTV.dial :air @00:45:41.39 Flushing upper manifold @00:45:41.52 CSV.dial :flush @00:45:42.25 CS.seek 5ml @00:45:54.44 delay 5 seconds @00:45:59.46 CSV.dial :air @00:46:00.01 CS.seek 6.999ml @00:46:05.65 CSV.dial CBV @00:46:05.67
CBV.dial CSV @00:46:06.70 CTV.dial :air @00:46:06.72 CS.seek 0ml @00:46:22.84 delay 7 seconds @00:46:29.88 CS.seek 3ml @00:46:37.46 CSV.dial :waste @00:46:37.93 CS.seek 0ml @00:46:45.58 CSV.dial :flush @00:46:46.05 CS.seek 3ml @00:46:54.28 delay 5 seconds @00:46:59.30 CSV.dial :air @00:46:59.82 CS.seek 5ml @00:47:05.30 CSV.dial :air @00:47:05.33 CS.seek 0ml @00:47:17.22 delay 7 seconds @00:47:24.26 CS.seek 3ml @00:47:31.83 CSV.dial :waste @00:47:32.58 CS.seek 0ml @00:47:40.09 Garaging FlushPuck in Collection Clamp @00:47:40.16 Elbow.reconfigure TightElbow @00:47:40.21 Elbow.seek CC @00:47:40.48 Elbow.reconfigure LooseElbow @00:47:40.58 Forearm.seek CC @00:47:41.57 Hand.close @00:47:42.17 CC.seek :open @00:47:46.53 Forearm.seek :retract @00:47:47.56 Elbow.reconfigure TightElbow @00:47:47.61 Elbow.seek :garage @00:47:50.61 Elbow.reconfigure LooseElbow @00:47:50.71 Forearm.reconfigure TightForearm @00:47:50.76 Forearm.seek :garage @00:47:51.91 Hand.open @00:47:52.61 Forearm.reconfigure LooseForearm @00:47:52.66 Forearm.seek :retract @00:47:53.65 Starting stand-alone bac WCR @00:47:53.67 bac wcr sampling at most 2000ml @00:47:53.69 Whole Cell Archive sampling 2000ml @00:47:53.71 Loading wcr puck from tube 2 into Collection Clamp @00:47:54.00 SC.reconfigure SCloose @00:47:54.23 Elbow.reconfigure TightElbow @00:47:54.28 Elbow.seek 2 @00:47:56.33 Forearm.reconfigure TightForearm @00:47:56.38 Forearm.seek 2 + 700 counts @00:47:57.41 SE.reconfigure SEpick @00:47:57.49 SE.seek beamBreak @00:47:59.68 SE.configure SEconfig @00:47:59.84 Elbow.seek 2 @00:48:00.12 Forearm.seek 2 + 300 counts @00:48:00.39 Forearm.reconfigure LooseForearm @00:48:00.43 Hand.close @00:48:01.07 SE.seek :down @00:48:01.28 Forearm.seek :retract @00:48:02.25 Elbow.seek CC @00:48:03.13 SE.coast @00:48:03.92 Elbow.reconfigure LooseElbow @00:48:04.03 Forearm.reconfigure TightForearm @00:48:04.08 Forearm.seek CC @00:48:05.99 CC.seek :closed @00:48:10.23 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.82 @00:48:10.25 Hand.open @00:48:10.98 Forearm.reconfigure LooseForearm @00:48:11.03 Forearm.seek :retract @00:48:12.08 SC.coast @00:48:12.12 collectSample 2000ml w/bubble point of 55psi @00:48:12.13 Priming 7ml @00:48:12.14 Intake.select :close @00:48:12.15 Exhaust.select :close @00:48:12.17 SS.hold @00:48:12.39 Calibrating pressure and temperature sensors @00:48:12.40 CTV.dial :air @00:48:12.42 SSV.dial :spit,avoiding: :exhaust @00:48:12.43 SS.reconfigure SSfast @00:48:12.48 SS.seek 5ml @00:48:15.03 SSV.dial CBV @00:48:15.05 CBV.dial SSV @00:48:16.45 delay 3.8 seconds @00:48:20.39 SSV.dial :spit @00:48:21.72 SS.seek 0ml @00:48:24.50 SSV.dial CBV @00:48:25.81 CTV.dial :intake @00:48:26.56 SSV.dial CBV @00:48:26.59 CBV.dial SSV @00:48:26.61 Allowing 40s for 7.0ml of remaining 7.0ml @00:48:26.62 Intake.select :open @00:48:28.79 Intake @31.5psia, vacuum from 20.0 to 24.0psi @00:48:28.81 SS.reconfigure SSinhale.with maxSpeed: 150 @00:48:28.86 SS.seek 7ml @00:48:32.51 Vacuum too high -- waiting up to 40s @00:48:35.55 Intake @31.3psia, vacuum from 20.0 to 24.0psi @00:48:35.57 SS.reconfigure SSinhale.with maxSpeed: 135 @00:48:35.62 SS.seek 7ml @00:48:38.10 Stroke completed -- waiting for pressure to equalize ... @00:48:42.52 Intake.select :close @00:48:42.67 SSV.dial :exhaust @00:48:43.46 Exhaust.select :open @00:48:43.57 SS.reconfigure SSexhale @00:48:43.62 SS.seek 0ml @00:48:47.32 Exhaust.select :close @00:48:47.44 Sampling 2000.0ml @00:48:47.45 Intake.select :close @00:48:47.46 Exhaust.select :close @00:48:47.48 SS.reconfigure SSconfig @00:48:48.07 SS.hold @00:48:48.31 CTV.dial :intake @00:48:48.33 SSV.dial CBV @00:48:48.36 CBV.dial SSV @00:48:49.16 Allowing 130s for 25.0ml of remaining 2000.0ml @00:48:49.17 Intake.select :open @00:48:51.35 Intake @32.1psia, vacuum from 20.0 to 24.0psi @00:48:51.37 SS.reconfigure SSinhale.with maxSpeed: 135 @00:48:51.42 SS.seek 25ml @00:48:54.98 Vacuum too high -- waiting up to 40s @00:48:56.80 Intake @31.6psia, vacuum from 20.0 to 24.0psi @00:48:56.82 SS.reconfigure SSinhale.with maxSpeed: 122 @00:48:56.87 SS.seek 25ml @00:48:59.67 Vacuum too high -- waiting up to 40s @00:49:01.73 Intake @31.7psia, vacuum from 20.0 to 24.0psi @00:49:01.75 SS.reconfigure SSinhale.with maxSpeed: 110 @00:49:01.80 SS.seek 25ml @00:49:04.85 Vacuum too high -- waiting up to 40s @00:49:06.66 Intake @31.7psia, vacuum from 20.0 to 24.0psi @00:49:06.68 SS.reconfigure SSinhale.with maxSpeed: 99 @00:49:06.73 SS.seek 25ml @00:49:09.82 Vacuum too high -- waiting up to 40s @00:49:11.62 Intake @31.5psia, vacuum from 20.0 to 24.0psi @00:49:11.64 SS.reconfigure SSinhale.with maxSpeed: 89 @00:49:11.69 SS.seek 25ml @00:49:15.23 Vacuum too high -- waiting up to 40s @00:49:17.07 Intake @31.7psia, vacuum from 20.0 to 24.0psi @00:49:17.09 SS.reconfigure SSinhale.with maxSpeed: 80 @00:49:17.14 SS.seek 25ml @00:49:20.70 Vacuum too high -- waiting up to 40s @00:49:22.27 Intake @31.6psia, vacuum from 20.0 to 24.0psi @00:49:22.29 SS.reconfigure SSinhale.with maxSpeed: 72 @00:49:22.34 SS.seek 25ml @00:49:25.83 Vacuum too high -- waiting up to 40s @00:49:27.65 Intake @31.5psia, vacuum from 20.0 to 24.0psi @00:49:27.67 SS.reconfigure SSinhale.with maxSpeed: 65 @00:49:27.72 SS.seek 25ml @00:49:29.91 Stroke completed -- waiting for pressure to equalize ... @00:49:33.10 Intake.select :close @00:49:33.26 SSV.dial :exhaust @00:49:34.03 Exhaust.select :open @00:49:34.14 SS.reconfigure SSexhale @00:49:34.19 SS.seek 0ml @00:49:42.99 Exhaust.select :close @00:49:43.11 SSV.dial CBV @00:49:43.14 CBV.dial SSV @00:49:43.94 Allowing 130s for 25.0ml of remaining 1975.0ml @00:49:43.95 Intake.select :open @00:49:46.12 Intake @32.1psia, vacuum from 20.0 to 24.0psi @00:49:46.14 SS.reconfigure SSinhale.with maxSpeed: 65 @00:49:46.19 SS.seek 25ml @00:49:51.82 Vacuum too high -- waiting up to 40s @00:49:53.38 Intake @32.1psia, vacuum from 20.0 to 24.0psi @00:49:53.39 SS.reconfigure SSinhale.with maxSpeed: 59 @00:49:53.44 SS.seek 25ml @00:49:58.67 Vacuum too high -- waiting up to 40s @00:50:00.21 Intake @32.0psia, vacuum from 20.0 to 24.0psi @00:50:00.23 SS.reconfigure SSinhale.with maxSpeed: 53 @00:50:00.28 SS.seek 25ml @00:50:07.57 Vacuum too high -- waiting up to 40s @00:50:08.86 Intake @31.4psia, vacuum from 20.0 to 24.0psi @00:50:08.88 SS.reconfigure SSinhale.with maxSpeed: 48 @00:50:08.93 SS.seek 25ml @00:50:24.20 Vacuum too high -- waiting up to 40s @00:50:26.04 Intake @31.7psia, vacuum from 20.0 to 24.0psi @00:50:26.06 SS.reconfigure SSinhale.with maxSpeed: 43 @00:50:26.11 SS.seek 25ml @00:50:28.77 Stroke completed -- waiting for pressure to equalize ... @00:50:31.96 Intake.select :close @00:50:32.12 SSV.dial :exhaust @00:50:32.89 Exhaust.select :open @00:50:33.00 SS.reconfigure SSexhale @00:50:33.05 SS.seek 0ml @00:50:41.71 Exhaust.select :close @00:50:41.83 SSV.dial CBV @00:50:41.86 CBV.dial SSV @00:50:42.67 Allowing 130s for 25.0ml of remaining 1950.0ml @00:50:42.68 Intake.select :open @00:50:44.85 Intake @32.1psia, vacuum from 20.0 to 24.0psi @00:50:44.87 SS.reconfigure SSinhale.with maxSpeed: 43 @00:50:44.92 SS.seek 25ml @00:51:26.35 Stroke completed -- waiting for pressure to equalize ... @00:51:30.15 Intake.select :close @00:51:30.31 SSV.dial :exhaust @00:51:31.06 Exhaust.select :open @00:51:31.17 SS.reconfigure SSexhale @00:51:31.22 SS.seek 0ml @00:51:40.16 Exhaust.select :close @00:51:40.28 SSV.dial CBV @00:51:40.31 CBV.dial SSV @00:51:41.11 Allowing 130s for 25.0ml of remaining 1925.0ml @00:51:41.12 Intake.select :open @00:51:43.29 Intake @32.0psia, vacuum from 20.0 to 24.0psi @00:51:43.31 SS.reconfigure SSinhale.with maxSpeed: 43 @00:51:43.36 SS.seek 25ml @00:52:24.50 Stroke completed -- waiting for pressure to equalize ... @00:52:28.29 Intake.select :close @00:52:28.44 SSV.dial :exhaust @00:52:29.22 Exhaust.select :open @00:52:29.33 SS.reconfigure SSexhale @00:52:29.38 SS.seek 0ml @00:52:38.30 Exhaust.select :close @00:52:38.41 SSV.dial CBV @00:52:38.44 CBV.dial SSV @00:52:39.23 Allowing 130s for 25.0ml of remaining 1900.0ml @00:52:39.24 Intake.select :open @00:52:41.41 Intake @32.0psia, vacuum from 20.0 to 24.0psi @00:52:41.43 SS.reconfigure SSinhale.with maxSpeed: 43 @00:52:41.48 SS.seek 25ml @00:53:22.71 Stroke completed -- waiting for pressure to equalize ... @00:53:25.50 GoodNews.email "Then, in tube 2: wcrPhase2\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:134\n{1 => 13, 2 => 9}\nCan@22:45:35, 17.0C, 78% humidity, 14.6psia, 14.237V, 0.468A, 0.468A avg, 6.66W",Subject: "Asleep until 00:43CEST13-Sep-19" @00:53:26.02 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @00:53:26.04 Retry #6 of 10 after 10:00 @00:53:26.86 Intake.select :close @00:53:27.02 SSV.dial :exhaust @00:53:27.81 Exhaust.select :open @00:53:27.92 SS.reconfigure SSexhale @00:53:27.97 SS.seek 0ml @00:53:36.82 Exhaust.select :close @00:53:36.94 SSV.dial CBV @00:53:36.97 CBV.dial SSV @00:53:37.78 Allowing 130s for 25.0ml of remaining 1875.0ml @00:53:37.79 Intake.select :open @00:53:39.96 Intake @32.2psia, vacuum from 20.0 to 24.0psi @00:53:39.98 SS.reconfigure SSinhale.with maxSpeed: 43 @00:53:40.03 SS.seek 25ml @00:54:21.52 Stroke completed -- waiting for pressure to equalize ... @00:54:25.32 Intake.select :close @00:54:25.48 SSV.dial :exhaust @00:54:26.26 Exhaust.select :open @00:54:26.37 SS.reconfigure SSexhale @00:54:26.42 SS.seek 0ml @00:54:35.42 Exhaust.select :close @00:54:35.53 SSV.dial CBV @00:54:35.56 CBV.dial SSV @00:54:36.36 Allowing 130s for 25.0ml of remaining 1850.0ml @00:54:36.37 Intake.select :open @00:54:38.54 Intake @32.4psia, vacuum from 20.0 to 24.0psi @00:54:38.56 SS.reconfigure SSinhale.with maxSpeed: 43 @00:54:38.61 SS.seek 25ml @00:55:19.82 Stroke completed -- waiting for pressure to equalize ... @00:55:23.61 Intake.select :close @00:55:23.77 SSV.dial :exhaust @00:55:24.53 Exhaust.select :open @00:55:24.64 SS.reconfigure SSexhale @00:55:24.69 SS.seek 0ml @00:55:33.63 Exhaust.select :close @00:55:33.75 SSV.dial CBV @00:55:33.78 CBV.dial SSV @00:55:34.58 Allowing 130s for 25.0ml of remaining 1825.0ml @00:55:34.59 Intake.select :open @00:55:36.76 Intake @31.9psia, vacuum from 20.0 to 24.0psi @00:55:36.78 SS.reconfigure SSinhale.with maxSpeed: 43 @00:55:36.83 SS.seek 25ml @00:56:18.10 Stroke completed -- waiting for pressure to equalize ... @00:56:21.89 Intake.select :close @00:56:22.05 SSV.dial :exhaust @00:56:22.82 Exhaust.select :open @00:56:22.94 SS.reconfigure SSexhale @00:56:23.00 SS.seek 0ml @00:56:31.97 Exhaust.select :close @00:56:32.09 SSV.dial CBV @00:56:32.12 CBV.dial SSV @00:56:33.41 Allowing 130s for 25.0ml of remaining 1800.0ml @00:56:33.42 Intake.select :open @00:56:35.59 Intake @32.2psia, vacuum from 20.0 to 24.0psi @00:56:35.61 SS.reconfigure SSinhale.with maxSpeed: 43 @00:56:35.66 SS.seek 25ml @00:57:16.93 Stroke completed -- waiting for pressure to equalize ... @00:57:21.39 Intake.select :close @00:57:21.60 SSV.dial :exhaust @00:57:22.42 Exhaust.select :open @00:57:22.53 SS.reconfigure SSexhale @00:57:22.58 SS.seek 0ml @00:57:31.61 Exhaust.select :close @00:57:31.72 SSV.dial CBV @00:57:31.75 CBV.dial SSV @00:57:32.56 Allowing 130s for 25.0ml of remaining 1775.0ml @00:57:32.57 Intake.select :open @00:57:34.74 Intake @32.4psia, vacuum from 20.0 to 24.0psi @00:57:34.76 SS.reconfigure SSinhale.with maxSpeed: 43 @00:57:34.81 SS.seek 25ml @00:58:16.08 Stroke completed -- waiting for pressure to equalize ... @00:58:20.51 Intake.select :close @00:58:20.66 SSV.dial :exhaust @00:58:21.44 Exhaust.select :open @00:58:21.56 SS.reconfigure SSexhale @00:58:21.61 SS.seek 0ml @00:58:30.53 Exhaust.select :close @00:58:30.65 SSV.dial CBV @00:58:30.69 CBV.dial SSV @00:58:31.48 Allowing 130s for 25.0ml of remaining 1750.0ml @00:58:31.49 Intake.select :open @00:58:33.66 Intake @32.0psia, vacuum from 20.0 to 24.0psi @00:58:33.68 SS.reconfigure SSinhale.with maxSpeed: 43 @00:58:33.73 SS.seek 25ml @00:59:15.00 Stroke completed -- waiting for pressure to equalize ... @00:59:19.42 Intake.select :close @00:59:19.58 SSV.dial :exhaust @00:59:20.36 Exhaust.select :open @00:59:20.48 SS.reconfigure SSexhale @00:59:20.53 SS.seek 0ml @00:59:29.49 Exhaust.select :close @00:59:29.61 SSV.dial CBV @00:59:29.63 CBV.dial SSV @00:59:30.45 Allowing 130s for 25.0ml of remaining 1725.0ml @00:59:30.46 Intake.select :open @00:59:32.63 Intake @32.3psia, vacuum from 20.0 to 24.0psi @00:59:32.65 SS.reconfigure SSinhale.with maxSpeed: 43 @00:59:32.69 SS.seek 25ml @01:00:14.13 Stroke completed -- waiting for pressure to equalize ... @01:00:18.51 Intake.select :close @01:00:18.67 SSV.dial :exhaust @01:00:19.45 Exhaust.select :open @01:00:19.57 SS.reconfigure SSexhale @01:00:19.62 SS.seek 0ml @01:00:28.58 Exhaust.select :close @01:00:28.70 SSV.dial CBV @01:00:28.74 CBV.dial SSV @01:00:29.53 Allowing 130s for 25.0ml of remaining 1700.0ml @01:00:29.54 Intake.select :open @01:00:31.71 Intake @32.2psia, vacuum from 20.0 to 24.0psi @01:00:31.73 SS.reconfigure SSinhale.with maxSpeed: 43 @01:00:31.78 SS.seek 25ml @01:01:13.08 Stroke completed -- waiting for pressure to equalize ... @01:01:17.49 Intake.select :close @01:01:17.65 SSV.dial :exhaust @01:01:18.43 Exhaust.select :open @01:01:18.54 SS.reconfigure SSexhale @01:01:18.60 SS.seek 0ml @01:01:27.52 Exhaust.select :close @01:01:27.65 SSV.dial CBV @01:01:27.67 CBV.dial SSV @01:01:28.48 Allowing 130s for 25.0ml of remaining 1675.0ml @01:01:28.49 Intake.select :open @01:01:30.66 Intake @32.4psia, vacuum from 20.0 to 24.0psi @01:01:30.68 SS.reconfigure SSinhale.with maxSpeed: 43 @01:01:30.73 SS.seek 25ml @01:02:11.96 Stroke completed -- waiting for pressure to equalize ... @01:02:16.38 Intake.select :close @01:02:16.53 SSV.dial :exhaust @01:02:17.32 Exhaust.select :open @01:02:17.43 SS.reconfigure SSexhale @01:02:17.48 SS.seek 0ml @01:02:26.44 Exhaust.select :close @01:02:26.56 SSV.dial CBV @01:02:26.60 CBV.dial SSV @01:02:27.38 Allowing 130s for 25.0ml of remaining 1650.0ml @01:02:27.40 Intake.select :open @01:02:29.58 Intake @32.4psia, vacuum from 20.0 to 24.0psi @01:02:29.60 SS.reconfigure SSinhale.with maxSpeed: 43 @01:02:29.64 SS.seek 25ml @01:03:10.99 Stroke completed -- waiting for pressure to equalize ... @01:03:15.40 Intake.select :close @01:03:15.56 SSV.dial :exhaust @01:03:16.34 Exhaust.select :open @01:03:16.46 SS.reconfigure SSexhale @01:03:16.50 SS.seek 0ml @01:03:25.99 Exhaust.select :close @01:03:26.06 GoodNews.email "Then, in tube 2: wcrPhase2\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:134\n{1 => 13, 2 => 9}\nCan@22:45:35, 17.0C, 78% humidity, 14.6psia, 14.237V, 0.468A, 0.468A avg, 6.66W",Subject: "Asleep until 00:43CEST13-Sep-19" @01:03:26.09 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @01:03:26.10 Retry #7 of 10 after 10:00 @01:03:26.13 SSV.dial CBV @01:03:26.15 CBV.dial SSV @01:03:26.97 Allowing 130s for 25.0ml of remaining 1625.0ml @01:03:26.98 Intake.select :open @01:03:29.15 Intake @32.2psia, vacuum from 20.0 to 24.0psi @01:03:29.17 SS.reconfigure SSinhale.with maxSpeed: 43 @01:03:29.22 SS.seek 25ml @01:04:10.50 Stroke completed -- waiting for pressure to equalize ... @01:04:14.94 Intake.select :close @01:04:15.10 SSV.dial :exhaust @01:04:15.87 Exhaust.select :open @01:04:15.98 SS.reconfigure SSexhale @01:04:16.03 SS.seek 0ml @01:04:24.64 Exhaust.select :close @01:04:24.76 SSV.dial CBV @01:04:24.81 CBV.dial SSV @01:04:25.57 Allowing 130s for 25.0ml of remaining 1600.0ml @01:04:25.58 Intake.select :open @01:04:27.75 Intake @32.3psia, vacuum from 20.0 to 24.0psi @01:04:27.77 SS.reconfigure SSinhale.with maxSpeed: 43 @01:04:27.82 SS.seek 25ml @01:05:09.42 Stroke completed -- waiting for pressure to equalize ... @01:05:13.85 Intake.select :close @01:05:13.99 SSV.dial :exhaust @01:05:14.78 Exhaust.select :open @01:05:14.89 SS.reconfigure SSexhale @01:05:14.94 SS.seek 0ml @01:05:23.52 Exhaust.select :close @01:05:23.64 SSV.dial CBV @01:05:23.67 CBV.dial SSV @01:05:24.47 Allowing 130s for 25.0ml of remaining 1575.0ml @01:05:24.48 Intake.select :open @01:05:26.65 Intake @32.4psia, vacuum from 20.0 to 24.0psi @01:05:26.67 SS.reconfigure SSinhale.with maxSpeed: 43 @01:05:26.72 SS.seek 25ml @01:06:01.07 Vacuum too high -- waiting up to 40s @01:06:02.39 Intake @31.8psia, vacuum from 20.0 to 24.0psi @01:06:02.41 SS.reconfigure SSinhale.with maxSpeed: 39 @01:06:02.46 SS.seek 25ml @01:06:10.64 Stroke completed -- waiting for pressure to equalize ... @01:06:14.43 Intake.select :close @01:06:14.59 SSV.dial :exhaust @01:06:15.36 Exhaust.select :open @01:06:15.98 SS.reconfigure SSexhale @01:06:16.04 SS.seek 0ml @01:06:25.00 Exhaust.select :close @01:06:25.12 SSV.dial CBV @01:06:25.14 CBV.dial SSV @01:06:25.97 Allowing 130s for 25.0ml of remaining 1550.0ml @01:06:26.00 Intake.select :open @01:06:28.20 Intake @32.3psia, vacuum from 20.0 to 24.0psi @01:06:28.22 SS.reconfigure SSinhale.with maxSpeed: 39 @01:06:28.27 SS.seek 25ml @01:07:13.72 Stroke completed -- waiting for pressure to equalize ... @01:07:17.57 Intake.select :close @01:07:17.73 SSV.dial :exhaust @01:07:18.51 Exhaust.select :open @01:07:18.62 SS.reconfigure SSexhale @01:07:18.67 SS.seek 0ml @01:07:27.64 Exhaust.select :close @01:07:27.76 SSV.dial CBV @01:07:27.81 CBV.dial SSV @01:07:28.59 Allowing 130s for 25.0ml of remaining 1525.0ml @01:07:28.60 Intake.select :open @01:07:30.77 Intake @32.4psia, vacuum from 20.0 to 24.0psi @01:07:30.79 SS.reconfigure SSinhale.with maxSpeed: 39 @01:07:30.84 SS.seek 25ml @01:08:16.20 Stroke completed -- waiting for pressure to equalize ... @01:08:20.63 Intake.select :close @01:08:20.79 SSV.dial :exhaust @01:08:21.56 Exhaust.select :open @01:08:21.68 SS.reconfigure SSexhale @01:08:21.73 SS.seek 0ml @01:08:30.69 Exhaust.select :close @01:08:30.81 SSV.dial CBV @01:08:30.84 CBV.dial SSV @01:08:31.64 Allowing 130s for 25.0ml of remaining 1500.0ml @01:08:31.65 Intake.select :open @01:08:33.82 Intake @32.4psia, vacuum from 20.0 to 24.0psi @01:08:33.84 SS.reconfigure SSinhale.with maxSpeed: 39 @01:08:33.88 SS.seek 25ml @01:09:19.20 Stroke completed -- waiting for pressure to equalize ... @01:09:23.61 Intake.select :close @01:09:23.77 SSV.dial :exhaust @01:09:24.54 Exhaust.select :open @01:09:24.66 SS.reconfigure SSexhale @01:09:24.71 SS.seek 0ml @01:09:33.66 Exhaust.select :close @01:09:33.78 SSV.dial CBV @01:09:33.82 CBV.dial SSV @01:09:34.61 Allowing 130s for 25.0ml of remaining 1475.0ml @01:09:34.62 Intake.select :open @01:09:36.79 Intake @32.3psia, vacuum from 20.0 to 24.0psi @01:09:36.81 SS.reconfigure SSinhale.with maxSpeed: 39 @01:09:36.85 SS.seek 25ml @01:10:22.45 Stroke completed -- waiting for pressure to equalize ... @01:10:26.77 Intake.select :close @01:10:26.92 SSV.dial :exhaust @01:10:27.70 Exhaust.select :open @01:10:27.81 SS.reconfigure SSexhale @01:10:27.86 SS.seek 0ml @01:10:36.83 Exhaust.select :close @01:10:36.96 SSV.dial CBV @01:10:36.98 CBV.dial SSV @01:10:37.78 Allowing 130s for 25.0ml of remaining 1450.0ml @01:10:37.79 Intake.select :open @01:10:39.96 Intake @32.5psia, vacuum from 20.0 to 24.0psi @01:10:39.98 SS.reconfigure SSinhale.with maxSpeed: 39 @01:10:40.03 SS.seek 25ml @01:11:25.36 Stroke completed -- waiting for pressure to equalize ... @01:11:29.78 Intake.select :close @01:11:29.93 SSV.dial :exhaust @01:11:30.71 Exhaust.select :open @01:11:30.82 SS.reconfigure SSexhale @01:11:30.87 SS.seek 0ml @01:11:39.72 Exhaust.select :close @01:11:39.84 SSV.dial CBV @01:11:39.87 CBV.dial SSV @01:11:40.67 Allowing 130s for 25.0ml of remaining 1425.0ml @01:11:40.68 Intake.select :open @01:11:42.84 Intake @32.4psia, vacuum from 20.0 to 24.0psi @01:11:42.86 SS.reconfigure SSinhale.with maxSpeed: 39 @01:11:42.91 SS.seek 25ml @01:12:28.31 Stroke completed -- waiting for pressure to equalize ... @01:12:33.51 Intake.select :close @01:12:33.67 SSV.dial :exhaust @01:12:34.45 Exhaust.select :open @01:12:34.56 SS.reconfigure SSexhale @01:12:34.61 SS.seek 0ml @01:12:43.66 Exhaust.select :close @01:12:43.78 SSV.dial CBV @01:12:43.81 CBV.dial SSV @01:12:44.62 Allowing 130s for 25.0ml of remaining 1400.0ml @01:12:44.63 Intake.select :open @01:12:46.80 Intake @32.5psia, vacuum from 20.0 to 24.0psi @01:12:46.82 SS.reconfigure SSinhale.with maxSpeed: 39 @01:12:46.87 SS.seek 25ml @01:13:26.13 GoodNews.email "Then, in tube 2: wcrPhase2\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:134\n{1 => 13, 2 => 9}\nCan@22:45:35, 17.0C, 78% humidity, 14.6psia, 14.237V, 0.468A, 0.468A avg, 6.66W",Subject: "Asleep until 00:43CEST13-Sep-19" @01:13:26.16 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @01:13:26.17 Retry #8 of 10 after 10:00 @01:13:32.24 Stroke completed -- waiting for pressure to equalize ... @01:13:37.16 Intake.select :close @01:13:37.33 SSV.dial :exhaust @01:13:38.10 Exhaust.select :open @01:13:38.21 SS.reconfigure SSexhale @01:13:38.26 SS.seek 0ml @01:13:46.97 Exhaust.select :close @01:13:47.10 SSV.dial CBV @01:13:47.12 CBV.dial SSV @01:13:47.92 Allowing 130s for 25.0ml of remaining 1375.0ml @01:13:47.93 Intake.select :open @01:13:50.10 Intake @32.4psia, vacuum from 20.0 to 24.0psi @01:13:50.12 SS.reconfigure SSinhale.with maxSpeed: 39 @01:13:50.17 SS.seek 25ml @01:14:35.89 Stroke completed -- waiting for pressure to equalize ... @01:14:40.94 Intake.select :close @01:14:41.09 SSV.dial :exhaust @01:14:41.87 Exhaust.select :open @01:14:41.98 SS.reconfigure SSexhale @01:14:42.03 SS.seek 0ml @01:14:50.91 Exhaust.select :close @01:14:51.03 SSV.dial CBV @01:14:51.07 CBV.dial SSV @01:14:51.85 Allowing 130s for 25.0ml of remaining 1350.0ml @01:14:51.86 Intake.select :open @01:14:54.03 Intake @32.2psia, vacuum from 20.0 to 24.0psi @01:14:54.05 SS.reconfigure SSinhale.with maxSpeed: 39 @01:14:54.10 SS.seek 25ml @01:15:39.47 Stroke completed -- waiting for pressure to equalize ... @01:15:45.12 Intake.select :close @01:15:45.28 SSV.dial :exhaust @01:15:46.06 Exhaust.select :open @01:15:46.17 SS.reconfigure SSexhale @01:15:46.22 SS.seek 0ml @01:15:55.00 Exhaust.select :close @01:15:55.12 SSV.dial CBV @01:15:55.16 CBV.dial SSV @01:15:55.95 Allowing 130s for 25.0ml of remaining 1325.0ml @01:15:55.96 Intake.select :open @01:15:58.13 Intake @32.6psia, vacuum from 20.0 to 24.0psi @01:15:58.15 SS.reconfigure SSinhale.with maxSpeed: 39 @01:15:58.19 SS.seek 25ml @01:16:29.44 Vacuum too high -- waiting up to 40s @01:16:30.99 Intake @32.5psia, vacuum from 20.0 to 24.0psi @01:16:31.01 SS.reconfigure SSinhale.with maxSpeed: 35 @01:16:31.06 SS.seek 25ml @01:16:47.14 Stroke completed -- waiting for pressure to equalize ... @01:16:51.46 Intake.select :close @01:16:51.62 SSV.dial :exhaust @01:16:52.41 Exhaust.select :open @01:16:52.52 SS.reconfigure SSexhale @01:16:52.58 SS.seek 0ml @01:17:01.64 Exhaust.select :close @01:17:01.81 SSV.dial CBV @01:17:01.85 CBV.dial SSV @01:17:02.65 Allowing 130s for 25.0ml of remaining 1300.0ml @01:17:02.66 Intake.select :open @01:17:04.83 Intake @32.8psia, vacuum from 20.0 to 24.0psi @01:17:04.85 SS.reconfigure SSinhale.with maxSpeed: 35 @01:17:04.90 SS.seek 25ml @01:17:55.30 Stroke completed -- waiting for pressure to equalize ... @01:17:59.74 Intake.select :close @01:17:59.90 SSV.dial :exhaust @01:18:00.65 Exhaust.select :open @01:18:00.76 SS.reconfigure SSexhale @01:18:00.82 SS.seek 0ml @01:18:09.81 Exhaust.select :close @01:18:09.92 SSV.dial CBV @01:18:09.95 CBV.dial SSV @01:18:10.74 Allowing 130s for 25.0ml of remaining 1275.0ml @01:18:10.75 Intake.select :open @01:18:12.92 Intake @32.1psia, vacuum from 20.0 to 24.0psi @01:18:12.94 SS.reconfigure SSinhale.with maxSpeed: 35 @01:18:12.99 SS.seek 25ml @01:19:03.31 Stroke completed -- waiting for pressure to equalize ... @01:19:08.36 Intake.select :close @01:19:08.52 SSV.dial :exhaust @01:19:09.29 Exhaust.select :open @01:19:09.40 SS.reconfigure SSexhale @01:19:09.45 SS.seek 0ml @01:19:18.27 Exhaust.select :close @01:19:18.39 SSV.dial CBV @01:19:18.43 CBV.dial SSV @01:19:19.23 Allowing 130s for 25.0ml of remaining 1250.0ml @01:19:19.24 Intake.select :open @01:19:21.41 Intake @32.4psia, vacuum from 20.0 to 24.0psi @01:19:21.43 SS.reconfigure SSinhale.with maxSpeed: 35 @01:19:21.48 SS.seek 25ml @01:20:11.92 Stroke completed -- waiting for pressure to equalize ... @01:20:17.46 Intake.select :close @01:20:17.62 SSV.dial :exhaust @01:20:18.40 Exhaust.select :open @01:20:18.51 SS.reconfigure SSexhale @01:20:18.56 SS.seek 0ml @01:20:27.60 Exhaust.select :close @01:20:27.72 SSV.dial CBV @01:20:27.74 CBV.dial SSV @01:20:28.54 Allowing 130s for 25.0ml of remaining 1225.0ml @01:20:28.55 Intake.select :open @01:20:30.72 Intake @32.5psia, vacuum from 20.0 to 24.0psi @01:20:30.74 SS.reconfigure SSinhale.with maxSpeed: 35 @01:20:30.79 SS.seek 25ml @01:21:20.67 Stroke completed -- waiting for pressure to equalize ... @01:21:25.72 Intake.select :close @01:21:25.87 SSV.dial :exhaust @01:21:26.65 Exhaust.select :open @01:21:26.76 SS.reconfigure SSexhale @01:21:26.81 SS.seek 0ml @01:21:35.84 Exhaust.select :close @01:21:35.96 SSV.dial CBV @01:21:35.99 CBV.dial SSV @01:21:36.79 Allowing 130s for 25.0ml of remaining 1200.0ml @01:21:36.80 Intake.select :open @01:21:38.98 Intake @32.7psia, vacuum from 20.0 to 24.0psi @01:21:39.00 SS.reconfigure SSinhale.with maxSpeed: 35 @01:21:39.05 SS.seek 25ml @01:22:29.41 Stroke completed -- waiting for pressure to equalize ... @01:22:34.46 Intake.select :close @01:22:34.62 SSV.dial :exhaust @01:22:35.40 Exhaust.select :open @01:22:35.51 SS.reconfigure SSexhale @01:22:35.56 SS.seek 0ml @01:22:44.78 Exhaust.select :close @01:22:44.90 SSV.dial CBV @01:22:44.93 CBV.dial SSV @01:22:45.73 Allowing 130s for 25.0ml of remaining 1175.0ml @01:22:45.74 Intake.select :open @01:22:47.91 Intake @32.6psia, vacuum from 20.0 to 24.0psi @01:22:47.93 SS.reconfigure SSinhale.with maxSpeed: 35 @01:22:47.97 SS.seek 25ml @01:23:26.20 GoodNews.email "Then, in tube 2: wcrPhase2\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:134\n{1 => 13, 2 => 9}\nCan@22:45:35, 17.0C, 78% humidity, 14.6psia, 14.237V, 0.468A, 0.468A avg, 6.66W",Subject: "Asleep until 00:43CEST13-Sep-19" @01:23:26.23 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @01:23:26.24 Retry #9 of 10 after 10:00 @01:23:38.41 Stroke completed -- waiting for pressure to equalize ... @01:23:44.06 Intake.select :close @01:23:44.78 SSV.dial :exhaust @01:23:45.55 Exhaust.select :open @01:23:45.68 SS.reconfigure SSexhale @01:23:45.73 SS.seek 0ml @01:23:54.57 Exhaust.select :close @01:23:54.68 SSV.dial CBV @01:23:54.71 CBV.dial SSV @01:23:55.51 Allowing 130s for 25.0ml of remaining 1150.0ml @01:23:55.52 Intake.select :open @01:23:57.69 Intake @32.8psia, vacuum from 20.0 to 24.0psi @01:23:57.71 SS.reconfigure SSinhale.with maxSpeed: 35 @01:23:57.76 SS.seek 25ml @01:24:48.25 Stroke completed -- waiting for pressure to equalize ... @01:24:53.92 Intake.select :close @01:24:54.08 SSV.dial :exhaust @01:24:54.85 Exhaust.select :open @01:24:54.96 SS.reconfigure SSexhale @01:24:55.02 SS.seek 0ml @01:25:03.84 Exhaust.select :close @01:25:03.96 SSV.dial CBV @01:25:03.98 CBV.dial SSV @01:25:04.79 Allowing 130s for 25.0ml of remaining 1125.0ml @01:25:04.80 Intake.select :open @01:25:06.97 Intake @32.7psia, vacuum from 20.0 to 24.0psi @01:25:06.99 SS.reconfigure SSinhale.with maxSpeed: 35 @01:25:07.03 SS.seek 25ml @01:25:41.22 Vacuum too high -- waiting up to 40s @01:25:42.53 Intake @32.3psia, vacuum from 20.0 to 24.0psi @01:25:42.55 SS.reconfigure SSinhale.with maxSpeed: 32 @01:25:42.60 SS.seek 25ml @01:26:00.47 Stroke completed -- waiting for pressure to equalize ... @01:26:05.69 Intake.select :close @01:26:05.92 SSV.dial :exhaust @01:26:06.71 Exhaust.select :open @01:26:06.82 SS.reconfigure SSexhale @01:26:06.87 SS.seek 0ml @01:26:15.97 Exhaust.select :close @01:26:16.09 SSV.dial CBV @01:26:16.12 CBV.dial SSV @01:26:16.92 Allowing 130s for 25.0ml of remaining 1100.0ml @01:26:16.93 Intake.select :open @01:26:19.10 Intake @33.0psia, vacuum from 20.0 to 24.0psi @01:26:19.12 SS.reconfigure SSinhale.with maxSpeed: 32 @01:26:19.17 SS.seek 25ml @01:27:14.19 Stroke completed -- waiting for pressure to equalize ... @01:27:19.75 Intake.select :close @01:27:19.91 SSV.dial :exhaust @01:27:20.68 Exhaust.select :open @01:27:20.79 SS.reconfigure SSexhale @01:27:20.84 SS.seek 0ml @01:27:29.72 Exhaust.select :close @01:27:29.84 SSV.dial CBV @01:27:29.88 CBV.dial SSV @01:27:30.65 Allowing 130s for 25.0ml of remaining 1075.0ml @01:27:30.66 Intake.select :open @01:27:32.83 Intake @33.0psia, vacuum from 20.0 to 24.0psi @01:27:32.85 SS.reconfigure SSinhale.with maxSpeed: 32 @01:27:32.90 SS.seek 25ml @01:28:27.90 Stroke completed -- waiting for pressure to equalize ... @01:28:33.58 Intake.select :close @01:28:33.73 SSV.dial :exhaust @01:28:34.51 Exhaust.select :open @01:28:34.62 SS.reconfigure SSexhale @01:28:34.67 SS.seek 0ml @01:28:43.76 Exhaust.select :close @01:28:43.88 SSV.dial CBV @01:28:43.92 CBV.dial SSV @01:28:44.71 Allowing 130s for 25.0ml of remaining 1050.0ml @01:28:44.72 Intake.select :open @01:28:46.89 Intake @32.6psia, vacuum from 20.0 to 24.0psi @01:28:46.91 SS.reconfigure SSinhale.with maxSpeed: 32 @01:28:46.95 SS.seek 25ml @01:29:41.97 Stroke completed -- waiting for pressure to equalize ... @01:29:48.25 Intake.select :close @01:29:48.40 SSV.dial :exhaust @01:29:49.18 Exhaust.select :open @01:29:49.29 SS.reconfigure SSexhale @01:29:49.34 SS.seek 0ml @01:29:57.97 Exhaust.select :close @01:29:58.09 SSV.dial CBV @01:29:58.12 CBV.dial SSV @01:29:58.91 Allowing 130s for 25.0ml of remaining 1025.0ml @01:29:58.92 Intake.select :open @01:30:01.09 Intake @33.1psia, vacuum from 20.0 to 24.0psi @01:30:01.11 SS.reconfigure SSinhale.with maxSpeed: 32 @01:30:01.16 SS.seek 25ml @01:30:56.50 Stroke completed -- waiting for pressure to equalize ... @01:31:02.74 Intake.select :close @01:31:02.90 SSV.dial :exhaust @01:31:03.68 Exhaust.select :open @01:31:03.79 SS.reconfigure SSexhale @01:31:03.84 SS.seek 0ml @01:31:12.49 Exhaust.select :close @01:31:12.61 SSV.dial CBV @01:31:12.63 CBV.dial SSV @01:31:13.41 Allowing 130s for 25.0ml of remaining 1000.0ml @01:31:13.42 Intake.select :open @01:31:15.59 Intake @33.0psia, vacuum from 20.0 to 24.0psi @01:31:15.61 SS.reconfigure SSinhale.with maxSpeed: 32 @01:31:15.65 SS.seek 25ml @01:32:05.06 Vacuum too high -- waiting up to 40s @01:32:06.63 Intake @32.6psia, vacuum from 20.0 to 24.0psi @01:32:06.65 SS.reconfigure SSinhale.with maxSpeed: 29 @01:32:06.69 SS.seek 25ml @01:32:13.76 Stroke completed -- waiting for pressure to equalize ... @01:32:18.80 Intake.select :close @01:32:18.95 SSV.dial :exhaust @01:32:19.73 Exhaust.select :open @01:32:19.84 SS.reconfigure SSexhale @01:32:19.90 SS.seek 0ml @01:32:28.92 Exhaust.select :close @01:32:29.04 SSV.dial CBV @01:32:29.07 CBV.dial SSV @01:32:29.87 Allowing 130s for 25.0ml of remaining 975.0ml @01:32:29.88 Intake.select :open @01:32:32.05 Intake @33.1psia, vacuum from 20.0 to 24.0psi @01:32:32.07 SS.reconfigure SSinhale.with maxSpeed: 29 @01:32:32.12 SS.seek 25ml @01:33:26.26 GoodNews.email "Then, in tube 2: wcrPhase2\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:134\n{1 => 13, 2 => 9}\nCan@22:45:35, 17.0C, 78% humidity, 14.6psia, 14.237V, 0.468A, 0.468A avg, 6.66W",Subject: "Asleep until 00:43CEST13-Sep-19" @01:33:26.29 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @01:33:26.30 Retry #10 of 10 after 10:00 @01:33:32.65 Stroke completed -- waiting for pressure to equalize ... @01:33:38.31 Intake.select :close @01:33:38.46 SSV.dial :exhaust @01:33:39.26 Exhaust.select :open @01:33:39.42 SS.reconfigure SSexhale @01:33:39.52 SS.seek 0ml @01:33:48.58 Exhaust.select :close @01:33:48.70 SSV.dial CBV @01:33:49.19 CBV.dial SSV @01:33:50.02 Allowing 130s for 25.0ml of remaining 950.0ml @01:33:50.03 Intake.select :open @01:33:52.20 Intake @33.2psia, vacuum from 20.0 to 24.0psi @01:33:52.22 SS.reconfigure SSinhale.with maxSpeed: 29 @01:33:52.27 SS.seek 25ml @01:34:52.89 Stroke completed -- waiting for pressure to equalize ... @01:34:59.20 Intake.select :close @01:34:59.35 SSV.dial :exhaust @01:35:00.13 Exhaust.select :open @01:35:00.24 SS.reconfigure SSexhale @01:35:00.29 SS.seek 0ml @01:35:09.04 Exhaust.select :close @01:35:09.16 SSV.dial CBV @01:35:09.21 CBV.dial SSV @01:35:09.99 Allowing 130s for 25.0ml of remaining 925.0ml @01:35:10.00 Intake.select :open @01:35:12.17 Intake @32.8psia, vacuum from 20.0 to 24.0psi @01:35:12.19 SS.reconfigure SSinhale.with maxSpeed: 29 @01:35:12.24 SS.seek 25ml @01:36:12.83 Stroke completed -- waiting for pressure to equalize ... @01:36:19.75 Intake.select :close @01:36:19.91 SSV.dial :exhaust @01:36:20.68 Exhaust.select :open @01:36:20.79 SS.reconfigure SSexhale @01:36:20.84 SS.seek 0ml @01:36:29.83 Exhaust.select :close @01:36:29.95 SSV.dial CBV @01:36:29.98 CBV.dial SSV @01:36:30.77 Allowing 130s for 25.0ml of remaining 900.0ml @01:36:30.78 Intake.select :open @01:36:32.95 Intake @33.2psia, vacuum from 20.0 to 24.0psi @01:36:32.97 SS.reconfigure SSinhale.with maxSpeed: 29 @01:36:33.02 SS.seek 25ml @01:37:27.25 Vacuum too high -- waiting up to 40s @01:37:28.80 Intake @32.7psia, vacuum from 20.0 to 24.0psi @01:37:28.82 SS.reconfigure SSinhale.with maxSpeed: 26 @01:37:28.86 SS.seek 25ml @01:37:36.56 Stroke completed -- waiting for pressure to equalize ... @01:37:42.76 Intake.select :close @01:37:42.92 SSV.dial :exhaust @01:37:43.69 Exhaust.select :open @01:37:43.80 SS.reconfigure SSexhale @01:37:43.86 SS.seek 0ml @01:37:52.83 Exhaust.select :close @01:37:52.95 SSV.dial CBV @01:37:53.00 CBV.dial SSV @01:37:53.77 Allowing 130s for 25.0ml of remaining 875.0ml @01:37:53.78 Intake.select :open @01:37:55.95 Intake @32.8psia, vacuum from 20.0 to 24.0psi @01:37:55.97 SS.reconfigure SSinhale.with maxSpeed: 26 @01:37:56.02 SS.seek 25ml @01:39:03.53 Stroke completed -- waiting for pressure to equalize ... @01:39:09.81 Intake.select :close @01:39:09.96 SSV.dial :exhaust @01:39:10.74 Exhaust.select :open @01:39:10.85 SS.reconfigure SSexhale @01:39:10.90 SS.seek 0ml @01:39:19.97 Exhaust.select :close @01:39:20.09 SSV.dial CBV @01:39:20.13 CBV.dial SSV @01:39:20.91 Allowing 130s for 25.0ml of remaining 850.0ml @01:39:20.92 Intake.select :open @01:39:23.09 Intake @32.8psia, vacuum from 20.0 to 24.0psi @01:39:23.11 SS.reconfigure SSinhale.with maxSpeed: 26 @01:39:23.15 SS.seek 25ml @01:40:30.82 Stroke completed -- waiting for pressure to equalize ... @01:40:37.73 Intake.select :close @01:40:37.88 SSV.dial :exhaust @01:40:38.66 Exhaust.select :open @01:40:38.77 SS.reconfigure SSexhale @01:40:38.82 SS.seek 0ml @01:40:47.87 Exhaust.select :close @01:40:47.99 SSV.dial CBV @01:40:48.02 CBV.dial SSV @01:40:48.82 Allowing 130s for 25.0ml of remaining 825.0ml @01:40:48.83 Intake.select :open @01:40:51.00 Intake @32.8psia, vacuum from 20.0 to 24.0psi @01:40:51.02 SS.reconfigure SSinhale.with maxSpeed: 26 @01:40:51.06 SS.seek 25ml @01:41:58.65 Stroke completed -- waiting for pressure to equalize ... @01:42:06.09 Intake.select :close @01:42:06.25 SSV.dial :exhaust @01:42:07.02 Exhaust.select :open @01:42:07.13 SS.reconfigure SSexhale @01:42:07.18 SS.seek 0ml @01:42:16.23 Exhaust.select :close @01:42:16.35 SSV.dial CBV @01:42:16.38 CBV.dial SSV @01:42:17.15 Allowing 130s for 25.0ml of remaining 800.0ml @01:42:17.16 Intake.select :open @01:42:19.33 Intake @32.8psia, vacuum from 20.0 to 24.0psi @01:42:19.35 SS.reconfigure SSinhale.with maxSpeed: 26 @01:42:19.40 SS.seek 25ml @01:43:26.33 GoodNews.email "Then, in tube 2: wcrPhase2\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:134\n{1 => 13, 2 => 9}\nCan@22:45:35, 17.0C, 78% humidity, 14.6psia, 14.237V, 0.468A, 0.468A avg, 6.66W",Subject: "Asleep until 00:43CEST13-Sep-19" @01:43:26.36 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @01:43:26.37 GoodNews.email "At 00:43:40 began in tube 2: wcrPhase2\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:134\n{1 => 13, 2 => 9}\nCan@00:43:40, 16.4C, 80% humidity, 14.6psia, 14.198V, 0.777A, 0.777A avg, 11.03W",Subject: "Started TotaldeploymentFINAL3 phase wcrPhase2" @01:43:26.40 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @01:43:26.41 Retry #1 of 10 after 10:00 @01:43:26.87 Stroke completed -- waiting for pressure to equalize ... @01:43:34.99 Intake.select :close @01:43:35.14 SSV.dial :exhaust @01:43:35.93 Exhaust.select :open @01:43:36.04 SS.reconfigure SSexhale @01:43:36.09 SS.seek 0ml @01:43:45.20 Exhaust.select :close @01:43:45.32 SSV.dial CBV @01:43:45.35 CBV.dial SSV @01:43:46.15 Allowing 130s for 25.0ml of remaining 775.0ml @01:43:46.16 Intake.select :open @01:43:48.33 Intake @33.0psia, vacuum from 20.0 to 24.0psi @01:43:48.35 SS.reconfigure SSinhale.with maxSpeed: 26 @01:43:48.39 SS.seek 25ml @01:44:43.72 Vacuum too high -- waiting up to 40s @01:44:45.49 Intake @32.9psia, vacuum from 20.0 to 24.0psi @01:44:45.51 SS.reconfigure SSinhale.with maxSpeed: 23 @01:44:45.56 SS.seek 25ml @01:44:59.51 Stroke completed -- waiting for pressure to equalize ... @01:45:06.32 Intake.select :close @01:45:06.48 SSV.dial :exhaust @01:45:07.26 Exhaust.select :open @01:45:07.37 SS.reconfigure SSexhale @01:45:07.42 SS.seek 0ml @01:45:16.68 Exhaust.select :close @01:45:16.79 SSV.dial CBV @01:45:16.81 CBV.dial SSV @01:45:17.62 Allowing 130s for 25.0ml of remaining 750.0ml @01:45:17.63 Intake.select :open @01:45:19.80 Intake @33.0psia, vacuum from 20.0 to 24.0psi @01:45:19.82 SS.reconfigure SSinhale.with maxSpeed: 23 @01:45:19.87 SS.seek 25ml @01:46:36.01 Stroke completed -- waiting for pressure to equalize ... @01:46:43.52 Intake.select :close @01:46:43.67 SSV.dial :exhaust @01:46:44.44 Exhaust.select :open @01:46:44.55 SS.reconfigure SSexhale @01:46:44.60 SS.seek 0ml @01:46:53.68 Exhaust.select :close @01:46:53.80 SSV.dial CBV @01:46:53.83 CBV.dial SSV @01:46:54.65 Allowing 130s for 25.0ml of remaining 725.0ml @01:46:54.66 Intake.select :open @01:46:56.83 Intake @33.2psia, vacuum from 20.0 to 24.0psi @01:46:56.85 SS.reconfigure SSinhale.with maxSpeed: 23 @01:46:56.89 SS.seek 25ml @01:48:12.95 Stroke completed -- waiting for pressure to equalize ... @01:48:21.07 Intake.select :close @01:48:21.22 SSV.dial :exhaust @01:48:22.01 Exhaust.select :open @01:48:22.12 SS.reconfigure SSexhale @01:48:22.17 SS.seek 0ml @01:48:30.87 Exhaust.select :close @01:48:31.50 SSV.dial CBV @01:48:31.52 CBV.dial SSV @01:48:32.33 Allowing 130s for 25.0ml of remaining 700.0ml @01:48:32.34 Intake.select :open @01:48:34.51 Intake @32.9psia, vacuum from 20.0 to 24.0psi @01:48:34.53 SS.reconfigure SSinhale.with maxSpeed: 23 @01:48:34.58 SS.seek 25ml @01:49:51.07 Stroke completed -- waiting for pressure to equalize ... @01:49:59.84 Intake.select :close @01:50:00.00 SSV.dial :exhaust @01:50:00.76 Exhaust.select :open @01:50:00.88 SS.reconfigure SSexhale @01:50:00.93 SS.seek 0ml @01:50:09.95 Exhaust.select :close @01:50:10.08 SSV.dial CBV @01:50:10.10 CBV.dial SSV @01:50:10.90 Allowing 130s for 25.0ml of remaining 675.0ml @01:50:10.91 Intake.select :open @01:50:13.08 Intake @33.1psia, vacuum from 20.0 to 24.0psi @01:50:13.10 SS.reconfigure SSinhale.with maxSpeed: 23 @01:50:13.15 SS.seek 25ml @01:51:06.31 Vacuum too high -- waiting up to 40s @01:51:08.12 Intake @32.8psia, vacuum from 20.0 to 24.0psi @01:51:08.13 SS.reconfigure SSinhale.with maxSpeed: 21 @01:51:08.18 SS.seek 25ml @01:51:33.93 Stroke completed -- waiting for pressure to equalize ... @01:51:42.71 Intake.select :close @01:51:42.86 SSV.dial :exhaust @01:51:43.65 Exhaust.select :open @01:51:43.76 SS.reconfigure SSexhale @01:51:43.81 SS.seek 0ml @01:51:52.89 Exhaust.select :close @01:51:53.52 SSV.dial CBV @01:51:53.55 CBV.dial SSV @01:51:54.36 Allowing 130s for 25.0ml of remaining 650.0ml @01:51:54.37 Intake.select :open @01:51:56.54 Intake @33.1psia, vacuum from 20.0 to 24.0psi @01:51:56.56 SS.reconfigure SSinhale.with maxSpeed: 21 @01:51:56.61 SS.seek 25ml @01:53:19.93 Stroke completed -- waiting for pressure to equalize ... @01:53:26.43 GoodNews.email "At 00:43:40 began in tube 2: wcrPhase2\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:134\n{1 => 13, 2 => 9}\nCan@00:43:40, 16.4C, 80% humidity, 14.6psia, 14.198V, 0.777A, 0.777A avg, 11.03W",Subject: "Started TotaldeploymentFINAL3 phase wcrPhase2" @01:53:26.46 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @01:53:26.47 Retry #2 of 10 after 10:00 @01:53:29.35 Intake.select :close @01:53:29.50 SSV.dial :exhaust @01:53:30.29 Exhaust.select :open @01:53:30.40 SS.reconfigure SSexhale @01:53:30.45 SS.seek 0ml @01:53:39.45 Exhaust.select :close @01:53:39.57 SSV.dial CBV @01:53:39.60 CBV.dial SSV @01:53:40.43 Allowing 130s for 25.0ml of remaining 624.9ml @01:53:40.44 Intake.select :open @01:53:42.61 Intake @32.8psia, vacuum from 20.0 to 24.0psi @01:53:42.63 SS.reconfigure SSinhale.with maxSpeed: 21 @01:53:42.68 SS.seek 25ml @01:55:06.07 Stroke completed -- waiting for pressure to equalize ... @01:55:16.71 Intake.select :close @01:55:16.86 SSV.dial :exhaust @01:55:17.64 Exhaust.select :open @01:55:17.75 SS.reconfigure SSexhale @01:55:17.80 SS.seek 0ml @01:55:27.01 Exhaust.select :close @01:55:27.13 SSV.dial CBV @01:55:27.15 CBV.dial SSV @01:55:27.96 Allowing 130s for 25.0ml of remaining 599.9ml @01:55:27.97 Intake.select :open @01:55:30.14 Intake @33.1psia, vacuum from 20.0 to 24.0psi @01:55:30.16 SS.reconfigure SSinhale.with maxSpeed: 21 @01:55:30.70 SS.seek 25ml @01:56:13.90 Vacuum too high -- waiting up to 40s @01:56:15.98 Intake @32.8psia, vacuum from 20.0 to 24.0psi @01:56:16.00 SS.reconfigure SSinhale.with maxSpeed: 19 @01:56:16.05 SS.seek 25ml @01:57:00.79 Stroke completed -- waiting for pressure to equalize ... @01:57:11.43 Intake.select :close @01:57:11.59 SSV.dial :exhaust @01:57:12.36 Exhaust.select :open @01:57:12.47 SS.reconfigure SSexhale @01:57:12.53 SS.seek 0ml @01:57:21.63 Exhaust.select :close @01:57:21.75 SSV.dial CBV @01:57:21.78 CBV.dial SSV @01:57:22.58 Allowing 130s for 25.0ml of remaining 574.9ml @01:57:22.59 Intake.select :open @01:57:24.76 Intake @33.1psia, vacuum from 20.0 to 24.0psi @01:57:24.78 SS.reconfigure SSinhale.with maxSpeed: 19 @01:57:24.83 SS.seek 25ml @01:58:56.84 Stroke completed -- waiting for pressure to equalize ... @01:59:09.22 Intake.select :close @01:59:09.38 SSV.dial :exhaust @01:59:10.16 Exhaust.select :open @01:59:10.27 SS.reconfigure SSexhale @01:59:10.33 SS.seek 0ml @01:59:19.40 Exhaust.select :close @01:59:19.53 SSV.dial CBV @01:59:19.55 CBV.dial SSV @01:59:20.35 Allowing 130s for 25.0ml of remaining 549.9ml @01:59:20.36 Intake.select :open @01:59:22.53 Intake @33.1psia, vacuum from 20.0 to 24.0psi @01:59:22.55 SS.reconfigure SSinhale.with maxSpeed: 19 @01:59:22.60 SS.seek 25ml @02:00:22.78 Vacuum too high -- waiting up to 40s @02:00:24.84 Intake @32.8psia, vacuum from 20.0 to 24.0psi @02:00:24.86 SS.reconfigure SSinhale.with maxSpeed: 17 @02:00:24.91 SS.seek 25ml @02:01:01.46 Stroke completed -- waiting for pressure to equalize ... @02:01:12.74 Intake.select :close @02:01:12.88 SSV.dial :exhaust @02:01:13.66 Exhaust.select :open @02:01:13.77 SS.reconfigure SSexhale @02:01:13.82 SS.seek 0ml @02:01:22.99 Exhaust.select :close @02:01:23.11 SSV.dial CBV @02:01:23.13 CBV.dial SSV @02:01:23.94 Allowing 130s for 25.0ml of remaining 524.9ml @02:01:23.95 Intake.select :open @02:01:26.12 Intake @32.9psia, vacuum from 20.0 to 24.0psi @02:01:26.14 SS.reconfigure SSinhale.with maxSpeed: 17 @02:01:26.19 SS.seek 25ml @02:03:09.24 Stroke completed -- waiting for pressure to equalize ... @02:03:22.88 Intake.select :close @02:03:23.04 SSV.dial :exhaust @02:03:23.82 Exhaust.select :open @02:03:23.93 SS.reconfigure SSexhale @02:03:23.98 SS.seek 0ml @02:03:26.50 GoodNews.email "At 00:43:40 began in tube 2: wcrPhase2\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:134\n{1 => 13, 2 => 9}\nCan@00:43:40, 16.4C, 80% humidity, 14.6psia, 14.198V, 0.777A, 0.777A avg, 11.03W",Subject: "Started TotaldeploymentFINAL3 phase wcrPhase2" @02:03:26.53 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @02:03:26.54 Retry #3 of 10 after 10:00 @02:03:32.70 Exhaust.select :close @02:03:32.82 SSV.dial CBV @02:03:32.85 CBV.dial SSV @02:03:33.66 Allowing 130s for 25.0ml of remaining 499.9ml @02:03:33.67 Intake.select :open @02:03:35.84 Intake @33.3psia, vacuum from 20.0 to 24.0psi @02:03:35.86 SS.reconfigure SSinhale.with maxSpeed: 17 @02:03:35.91 SS.seek 25ml @02:04:52.90 Vacuum too high -- waiting up to 40s @02:04:55.20 Intake @32.8psia, vacuum from 20.0 to 24.0psi @02:04:55.22 SS.reconfigure SSinhale.with maxSpeed: 15 @02:04:55.27 SS.seek 25ml @02:05:25.76 Stroke completed -- waiting for pressure to equalize ... @02:05:39.38 Intake.select :close @02:05:39.54 SSV.dial :exhaust @02:05:40.32 Exhaust.select :open @02:05:40.45 SS.reconfigure SSexhale @02:05:40.49 SS.seek 0ml @02:05:49.73 Exhaust.select :close @02:05:49.89 SSV.dial CBV @02:05:49.91 CBV.dial SSV @02:05:50.72 Allowing 130s for 25.0ml of remaining 474.9ml @02:05:50.73 Intake.select :open @02:05:52.90 Intake @33.1psia, vacuum from 20.0 to 24.0psi @02:05:52.92 SS.reconfigure SSinhale.with maxSpeed: 15 @02:05:52.97 SS.seek 25ml @02:07:49.70 Stroke completed -- waiting for pressure to equalize ... @02:08:00.74 SS.reconfigure SSconfig @02:08:00.80 SS.hold @02:08:00.92 Intake.select :close @02:08:01.08 ShallowSampler::Clogged in WCR -- progress too slow after 1550.1ml @02:08:01.09 SSV.dial :exhaust @02:08:01.85 Exhaust.select :open @02:08:01.97 SS.reconfigure SSexhale @02:08:02.02 SS.seek 0ml @02:08:11.13 Exhaust.select :close @02:08:11.25 Sampled 1550.1ml @02:08:11.27 Drying puck before fixing for 4:00 @02:08:11.28 CBV.dial :waste2b @02:08:12.07 delay 1:00 @02:09:12.10 CSV.dial :air @02:09:12.84 CS.seek 10ml @02:09:35.90 Pumping wcr puck from tube 2 up to 55psi... @02:09:35.91 CBV.dial SSV @02:09:37.26 CBV.dial between CSV and waste2b @02:09:38.25 CSV.dial between air and CTV @02:09:38.59 CS.seek 6.92ml @02:09:46.37 CTV.dial CSV,avoiding: :air @02:09:46.40 CSV.dial CTV @02:09:47.20 CS.reconfigure CSpressurize @02:09:47.25 CS.seek 0ml @02:10:05.53 CSV.dial :air @02:10:06.00 CS.reconfigure CSfast @02:10:06.05 CS.seek 10ml @02:10:29.25 CSV.dial between air and CTV @02:10:30.10 CS.seek 2.497ml @02:10:47.56 CTV.dial CSV,avoiding: :air @02:10:47.59 CSV.dial CTV @02:10:47.90 CS.reconfigure CSpressurize @02:10:47.96 CS.seek 0ml @02:10:51.98 CBV.dial :waste2b @02:10:52.34 CS.reconfigure CSconfig @02:10:52.39 CS.seek :empty @02:10:56.15 delay 1:00 @02:11:56.18 CTV.dial :air,avoiding: :intake @02:11:57.48 Fix #1 of 2 (with rnal) for 2:00 @02:11:57.50 CSV.dial CSR @02:11:58.01 Pulling 2ml of CSR.rnal @02:11:58.02 CSR.select :rnal @02:11:58.14 CS.seek 2ml @02:12:04.31 delay 5 seconds @02:12:09.34 CSR.select :air @02:12:09.45 CS.reconfigure CSfast @02:12:09.50 CS.seek 10ml @02:12:27.87 Pumping wcr puck from tube 2 up to 55psi... @02:12:27.88 CBV.dial SSV @02:12:29.20 CBV.dial between CSV and waste2b @02:12:30.18 CSV.dial between air and CTV @02:12:30.81 CS.seek 6.944ml @02:12:38.69 CTV.dial CSV,avoiding: :air @02:12:38.73 CSV.dial CTV @02:12:40.08 CS.reconfigure CSpressurize @02:12:40.13 CS.seek 0ml @02:12:58.37 delay 2:00 @02:13:26.57 GoodNews.email "At 00:43:40 began in tube 2: wcrPhase2\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:134\n{1 => 13, 2 => 9}\nCan@00:43:40, 16.4C, 80% humidity, 14.6psia, 14.198V, 0.777A, 0.777A avg, 11.03W",Subject: "Started TotaldeploymentFINAL3 phase wcrPhase2" @02:13:26.60 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @02:13:26.61 Retry #4 of 10 after 10:00 @02:14:58.41 CBV.dial :waste2b @02:14:58.77 CS.reconfigure CSconfig @02:14:58.82 CS.seek :empty @02:14:59.41 delay 1:00 @02:15:59.44 CTV.dial :air,avoiding: :intake @02:16:00.78 Fix #2 of 2 (with rnal) for 2:00 @02:16:00.80 CSV.dial CSR @02:16:01.37 Pulling 2ml of CSR.rnal @02:16:01.38 CSR.select :rnal @02:16:01.50 CS.seek 2ml @02:16:07.60 delay 5 seconds @02:16:12.63 CSR.select :air @02:16:12.74 CS.reconfigure CSfast @02:16:12.80 CS.seek 10ml @02:16:31.16 Pumping wcr puck from tube 2 up to 55psi... @02:16:31.17 CBV.dial SSV @02:16:32.50 CBV.dial between CSV and waste2b @02:16:33.48 CSV.dial between air and CTV @02:16:34.23 CS.seek 6.916ml @02:16:42.00 CTV.dial CSV,avoiding: :air @02:16:42.02 CSV.dial CTV @02:16:43.41 CS.reconfigure CSpressurize @02:16:43.47 CS.seek 0ml @02:17:00.46 delay 2:00 @02:19:00.49 CBV.dial :waste2b @02:19:00.85 CS.reconfigure CSconfig @02:19:01.90 CS.seek :empty @02:19:04.07 delay 1:00 @02:20:04.10 CTV.dial :air,avoiding: :intake @02:20:05.47 Storing wcr puck in Collection Clamp to tube 1 @02:20:05.65 SC.reconfigure SCtight @02:20:05.70 SC.seek 1 @02:20:07.84 SC.reconfigure SCloose @02:20:08.07 Elbow.reconfigure TightElbow @02:20:08.12 Elbow.seek 1 @02:20:10.00 Forearm.reconfigure TightForearm @02:20:10.05 Forearm.seek 1 + 700 counts @02:20:11.08 SE.reconfigure SEpick @02:20:11.16 SE.seek beamBreak @02:20:13.04 SE.configure SEconfig @02:20:13.21 Elbow.reconfigure LimpElbow @02:20:13.26 Forearm.reconfigure LooseForearm @02:20:13.31 Forearm.seek 1 @02:20:13.61 Elbow.hold @02:20:13.68 Elbow.reconfigure TightElbow @02:20:13.79 SE.seek 12.07 Pucks below Top Plate @02:20:14.33 Forearm.force -20 @02:20:14.37 Forearm.seek 1 + 348 counts @02:20:14.69 Forearm.reconfigure TightForearm @02:20:14.74 Forearm.seek 1 + 700 counts @02:20:15.08 SE.seek 13.07 Pucks below Top Plate @02:20:15.71 Forearm.reconfigure LooseForearm @02:20:15.76 Forearm.seek :retract @02:20:16.73 Elbow.seek CC @02:20:18.43 Elbow.reconfigure LooseElbow @02:20:18.53 Forearm.seek CC @02:20:19.59 Hand.close @02:20:20.18 CC.seek :open @02:20:24.66 Forearm.seek :retract @02:20:25.71 Elbow.reconfigure TightElbow @02:20:25.76 Elbow.seek 1 @02:20:27.63 Elbow.reconfigure LimpElbow @02:20:27.69 Forearm.seek 1 @02:20:28.71 Elbow.hold @02:20:28.78 Elbow.reconfigure TightElbow @02:20:28.83 Hand.open @02:20:29.54 Forearm.force -20 @02:20:30.06 Forearm.seek 1 + 348 counts @02:20:30.41 Forearm.reconfigure TightForearm @02:20:30.47 Forearm.seek 1 + 700 counts @02:20:30.88 SE.seek :down @02:20:32.18 Forearm.reconfigure LooseForearm @02:20:32.28 Forearm.seek :retract @02:20:32.45 SE.coast @02:20:33.23 SC.coast @02:20:33.27 Starting finalPurge @02:20:33.28 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @02:20:33.36 Hand.open @02:20:33.67 Elbow.seek FlushPuck::Garage @02:20:35.53 Elbow.reconfigure LooseElbow @02:20:35.64 Forearm.seek :garage @02:20:36.64 Hand.close @02:20:37.42 Forearm.seek :retract @02:20:38.40 Elbow.reconfigure TightElbow @02:20:38.45 Elbow.seek CC @02:20:41.29 Elbow.reconfigure LooseElbow @02:20:41.39 Forearm.reconfigure TightForearm @02:20:41.43 Forearm.seek CC @02:20:42.78 CC.seek :closed @02:20:47.02 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.78 @02:20:47.10 Hand.open @02:20:47.74 Forearm.reconfigure LooseForearm @02:20:47.78 Forearm.seek :retract @02:20:48.78 Flushing the Collection Valve::Manifold @02:20:48.91 CSV.dial CSR @02:20:49.42 Pulling 5ml of CSR.flush @02:20:49.43 CSR.select :flush @02:20:49.55 CS.seek 5ml @02:21:03.60 delay 5 seconds @02:21:08.63 CSR.select :air @02:21:08.79 CS.reconfigure CSfast @02:21:08.84 CS.seek 7.5ml @02:21:15.35 CSV.dial :waste @02:21:16.76 CS.reconfigure CSconfig @02:21:16.81 CS.seek 0ml @02:21:36.94 Flushing lower manifold's waste1 port @02:21:37.07 CSV.dial :flush @02:21:37.55 CS.reconfigure CSfast @02:21:37.60 CS.seek 5ml @02:21:49.60 delay 5 seconds @02:21:54.62 CSV.dial :air @02:21:55.14 CS.seek 7.001ml @02:22:00.63 CSV.dial CTV @02:22:00.65
CTV.dial CSV @02:22:02.51 CBV.dial :waste1 @02:22:03.36 CS.seek 0ml @02:22:19.82 Flushing lower manifold's waste2b port @02:22:20.02 CSV.dial :flush @02:22:20.77 CS.seek 5ml @02:22:32.76 delay 5 seconds @02:22:37.78 CSV.dial :air @02:22:38.31 CS.seek 7ml @02:22:43.76 CSV.dial CTV @02:22:43.78
CTV.dial CSV @02:22:44.24 CBV.dial :waste2b @02:22:45.02 CS.seek 0ml @02:23:01.29 Flushing upper manifold @02:23:01.42 CSV.dial :flush @02:23:02.17 CS.seek 5ml @02:23:14.29 delay 5 seconds @02:23:19.32 CSV.dial :air @02:23:19.86 CS.seek 7ml @02:23:25.51 CSV.dial CBV @02:23:25.53
CBV.dial CSV @02:23:26.64 CTV.dial :air @02:23:26.69 GoodNews.email "At 00:43:40 began in tube 2: wcrPhase2\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:134\n{1 => 13, 2 => 9}\nCan@00:43:40, 16.4C, 80% humidity, 14.6psia, 14.198V, 0.777A, 0.777A avg, 11.03W",Subject: "Started TotaldeploymentFINAL3 phase wcrPhase2" @02:23:26.73 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @02:23:26.76 Retry #5 of 10 after 10:00 @02:23:27.96
CS.seek 0ml @02:23:44.26 delay 7 seconds @02:23:51.29 CS.seek 3ml @02:23:58.84 CSV.dial :waste @02:23:59.31 CS.seek 0ml @02:24:06.94 CSV.dial :flush @02:24:07.41 CS.seek 3ml @02:24:14.97 delay 5 seconds @02:24:20.00 CSV.dial :air @02:24:20.52 CS.seek 5ml @02:24:26.06 CSV.dial :air @02:24:26.08 CS.seek 0ml @02:24:37.95 delay 7 seconds @02:24:44.98 CS.seek 3ml @02:24:52.59 CSV.dial :waste @02:24:53.35 CS.seek 0ml @02:25:00.82 Disinfecting external Intake with mfbkill @02:25:00.95 CSV.dial CSR @02:25:02.29 Pulling 8ml of CSR.mfbkill @02:25:02.30 CSR.select :mfbkill @02:25:02.42 CS.seek 8ml @02:25:21.20 delay 5 seconds @02:25:26.23 CSR.select :air @02:25:26.35 CS.seek 10ml @02:25:31.73 CSV.dial CBV @02:25:31.75
CBV.dial CSV @02:25:32.77 CTV.dial :intake @02:25:33.54 Intake.select :open @02:25:33.66 CS.seek 2ml @02:25:53.16 Intake.select :close @02:25:53.27 CTV.dial :air @02:25:54.02 CS.reconfigure CSconfig @02:25:54.07 CS.seek 0ml @02:26:00.10 Flushing lower manifold's waste1 port @02:26:00.11 CSV.dial CSR @02:26:01.19 Pulling 4ml of CSR.mfbkill @02:26:01.20 CSR.select :mfbkill @02:26:01.32 CS.seek 4ml @02:26:12.51 delay 5 seconds @02:26:17.53 CSR.select :air @02:26:17.64 CS.reconfigure CSfast @02:26:17.69 CS.seek 10ml @02:26:31.67 CSV.dial CTV @02:26:31.71
CTV.dial CSV @02:26:33.09 CBV.dial :waste1 @02:26:33.58 CS.seek 0ml @02:26:56.17 Flushing lower manifold's waste2b port @02:26:56.18 CSV.dial CSR @02:26:57.20 Pulling 4ml of CSR.mfbkill @02:26:57.21 CSR.select :mfbkill @02:26:57.33 CS.reconfigure CSconfig @02:26:57.37 CS.seek 4ml @02:27:08.64 delay 5 seconds @02:27:13.67 CSR.select :air @02:27:13.78 CS.reconfigure CSfast @02:27:13.83 CS.seek 10ml @02:27:27.95 CSV.dial CTV @02:27:27.97
CTV.dial CSV @02:27:28.45 CBV.dial :waste2b @02:27:29.24 CS.seek 0ml @02:27:51.95 Killing upper manifold @02:27:51.96 CSV.dial CSR @02:27:52.48 Pulling 4ml of CSR.mfbkill @02:27:52.49 CSR.select :mfbkill @02:27:52.61 CS.reconfigure CSconfig @02:27:52.65 CS.seek 4ml @02:28:03.85 delay 5 seconds @02:28:08.88 CSR.select :air @02:28:09.00 CS.reconfigure CSfast @02:28:09.05 CS.seek 10ml @02:28:23.14 CSV.dial CBV @02:28:23.16
CBV.dial CSV @02:28:24.26 CTV.dial :air @02:28:25.58 CS.seek 0ml @02:28:48.14 delay 7 seconds @02:28:55.18 CS.seek 3ml @02:29:02.92 CSV.dial :waste @02:29:03.37 CS.seek 0ml @02:29:10.95 Flushing upper manifold @02:29:11.08 CSV.dial :flush @02:29:11.55 CS.seek 5ml @02:29:23.42 delay 5 seconds @02:29:28.45 CSV.dial :air @02:29:28.97 CS.seek 7ml @02:29:34.32 CSV.dial CBV @02:29:34.34
CBV.dial CSV @02:29:35.37 CTV.dial :air @02:29:35.39 CS.seek 0ml @02:29:51.39 delay 7 seconds @02:29:58.43 CS.seek 3ml @02:30:05.94 CSV.dial :waste @02:30:06.41 CS.seek 0ml @02:30:14.12 CSV.dial :flush @02:30:14.59 CS.seek 3ml @02:30:22.14 delay 5 seconds @02:30:27.16 CSV.dial :air @02:30:27.67 CS.seek 5ml @02:30:33.03 CSV.dial :air @02:30:33.05 CS.seek 0ml @02:30:44.86 delay 7 seconds @02:30:51.90 CS.seek 3ml @02:30:59.54 CSV.dial :waste @02:31:00.29 CS.seek 0ml @02:31:07.81 Flushing lower manifold's waste1 port @02:31:08.07 CSV.dial :flush @02:31:08.58 CS.seek 5ml @02:31:20.39 delay 5 seconds @02:31:25.41 CSV.dial :air @02:31:25.93 CS.seek 7ml @02:31:31.29 CSV.dial CTV @02:31:31.31
CTV.dial CSV @02:31:32.69 CBV.dial :waste1 @02:31:33.15 CS.seek 0ml @02:31:49.17 Flushing lower manifold's waste2b port @02:31:49.30 CSV.dial :flush @02:31:50.05 CS.seek 5ml @02:32:02.04 delay 5 seconds @02:32:07.07 CSV.dial :air @02:32:08.09 CS.seek 7ml @02:32:13.55 CSV.dial CTV @02:32:13.59
CTV.dial CSV @02:32:14.03 CBV.dial :waste2b @02:32:14.82 CS.seek 0ml @02:32:30.95 Disinfecting external Intake with kill @02:32:31.08 CSV.dial CSR @02:32:31.63 Pulling 8ml of CSR.kill @02:32:31.64 CSR.select :kill @02:32:31.76 CS.seek 8ml @02:32:50.01 delay 5 seconds @02:32:55.04 CSR.select :air @02:32:55.17 CS.seek 10ml @02:33:00.61 CSV.dial CBV @02:33:00.66
CBV.dial CSV @02:33:01.84 CTV.dial :intake @02:33:02.59 Intake.select :open @02:33:02.71 CS.seek 2ml @02:33:22.00 Intake.select :close @02:33:22.12 CTV.dial :air @02:33:22.87 CS.reconfigure CSconfig @02:33:22.92 CS.seek 0ml @02:33:26.79 GoodNews.email "At 00:43:40 began in tube 2: wcrPhase2\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:134\n{1 => 13, 2 => 9}\nCan@00:43:40, 16.4C, 80% humidity, 14.6psia, 14.198V, 0.777A, 0.777A avg, 11.03W",Subject: "Started TotaldeploymentFINAL3 phase wcrPhase2" @02:33:26.82 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @02:33:26.83 Retry #6 of 10 after 10:00 @02:33:29.19
CSV.dial CSR @02:33:30.28 Pulling 5ml of CSR.flush @02:33:30.29 CSR.select :flush @02:33:30.41 CS.seek 5ml @02:33:44.23 delay 5 seconds @02:33:49.26 CSR.select :air @02:33:49.39 CS.seek 10ml @02:34:03.16 CSV.dial CBV @02:34:04.19 CS.seek 0ml @02:34:30.64 Garaging FlushPuck in Collection Clamp @02:34:30.71 Elbow.reconfigure TightElbow @02:34:30.76 Elbow.seek CC @02:34:31.03 Elbow.reconfigure LooseElbow @02:34:31.12 Forearm.seek CC @02:34:32.15 Hand.close @02:34:32.81 CC.seek :open @02:34:37.17 Forearm.seek :retract @02:34:38.22 Elbow.reconfigure TightElbow @02:34:38.27 Elbow.seek :garage @02:34:41.22 Elbow.reconfigure LooseElbow @02:34:41.32 Forearm.reconfigure TightForearm @02:34:41.37 Forearm.seek :garage @02:34:42.53 Hand.open @02:34:43.25 Forearm.reconfigure LooseForearm @02:34:43.30 Forearm.seek :retract @02:34:44.29 Completed [wcrPhase2] begun 00:43:40 @02:34:44.31 Preparing to sleep until Fri Sep 13 04:43:35 +0200 2019 @02:34:44.37 SS.coast @02:34:44.39
Asleep til 04:43 @02:34:44.44 Continuing at 04:43 @02:34:44.46 CC.coast @02:34:44.57 CS.coast @02:34:44.62 PC.coast @02:34:44.66 Forearm.coast @02:34:44.70 Elbow.coast @02:34:45.09 Gate.power :core,:OFF @02:34:45.10 Gate.power :raw,:OFF @02:34:45.11 Shutdown @02:43:26.86 GoodNews.email "At 00:43:40 began in tube 2: wcrPhase2\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:134\n{1 => 13, 2 => 9}\nCan@00:43:40, 16.4C, 80% humidity, 14.6psia, 14.198V, 0.777A, 0.777A avg, 11.03W",Subject: "Started TotaldeploymentFINAL3 phase wcrPhase2" @02:43:26.89 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @02:43:26.90 Retry #7 of 10 after 10:00 @02:53:26.93 GoodNews.email "At 00:43:40 began in tube 2: wcrPhase2\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:134\n{1 => 13, 2 => 9}\nCan@00:43:40, 16.4C, 80% humidity, 14.6psia, 14.198V, 0.777A, 0.777A avg, 11.03W",Subject: "Started TotaldeploymentFINAL3 phase wcrPhase2" @02:53:26.96 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @02:53:26.97 Retry #8 of 10 after 10:00 @03:03:27.00 GoodNews.email "At 00:43:40 began in tube 2: wcrPhase2\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:134\n{1 => 13, 2 => 9}\nCan@00:43:40, 16.4C, 80% humidity, 14.6psia, 14.198V, 0.777A, 0.777A avg, 11.03W",Subject: "Started TotaldeploymentFINAL3 phase wcrPhase2" @03:03:27.03 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @03:03:27.04 Retry #9 of 10 after 10:00 @03:13:27.07 GoodNews.email "At 00:43:40 began in tube 2: wcrPhase2\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:134\n{1 => 13, 2 => 9}\nCan@00:43:40, 16.4C, 80% humidity, 14.6psia, 14.198V, 0.777A, 0.777A avg, 11.03W",Subject: "Started TotaldeploymentFINAL3 phase wcrPhase2" @03:13:27.10 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @03:13:27.11 Retry #10 of 10 after 10:00 @03:23:27.14 GoodNews.email "At 00:43:40 began in tube 2: wcrPhase2\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:134\n{1 => 13, 2 => 9}\nCan@00:43:40, 16.4C, 80% humidity, 14.6psia, 14.198V, 0.777A, 0.777A avg, 11.03W",Subject: "Started TotaldeploymentFINAL3 phase wcrPhase2" @03:23:27.17 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @03:23:27.18 BACfans.email "Started stand-alone bac WCR in tube 2 at 00:47:53CEST13-Sep-19\n{1 => 13, 2 => 9}\nCan@00:44:41, 16.3C, 80% humidity, 14.6psia, 14.188V, 0.777A, 0.777A avg, 11.02W",Subject: "bac wcr sampling at most 2000ml" @03:23:27.21 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @03:23:27.22 Retry #1 of 10 after 10:00 @03:33:27.25 BACfans.email "Started stand-alone bac WCR in tube 2 at 00:47:53CEST13-Sep-19\n{1 => 13, 2 => 9}\nCan@00:44:41, 16.3C, 80% humidity, 14.6psia, 14.188V, 0.777A, 0.777A avg, 11.02W",Subject: "bac wcr sampling at most 2000ml" @03:33:27.28 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @03:33:27.29 Retry #2 of 10 after 10:00 @03:43:27.32 BACfans.email "Started stand-alone bac WCR in tube 2 at 00:47:53CEST13-Sep-19\n{1 => 13, 2 => 9}\nCan@00:44:41, 16.3C, 80% humidity, 14.6psia, 14.188V, 0.777A, 0.777A avg, 11.02W",Subject: "bac wcr sampling at most 2000ml" @03:43:27.83 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @03:43:27.85 Retry #3 of 10 after 10:00 @03:53:27.87 BACfans.email "Started stand-alone bac WCR in tube 2 at 00:47:53CEST13-Sep-19\n{1 => 13, 2 => 9}\nCan@00:44:41, 16.3C, 80% humidity, 14.6psia, 14.188V, 0.777A, 0.777A avg, 11.02W",Subject: "bac wcr sampling at most 2000ml" @03:53:27.90 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @03:53:27.91 Retry #4 of 10 after 10:00 @04:03:27.94 BACfans.email "Started stand-alone bac WCR in tube 2 at 00:47:53CEST13-Sep-19\n{1 => 13, 2 => 9}\nCan@00:44:41, 16.3C, 80% humidity, 14.6psia, 14.188V, 0.777A, 0.777A avg, 11.02W",Subject: "bac wcr sampling at most 2000ml" @04:03:27.97 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @04:03:27.98 Retry #5 of 10 after 10:00 @04:13:28.01 BACfans.email "Started stand-alone bac WCR in tube 2 at 00:47:53CEST13-Sep-19\n{1 => 13, 2 => 9}\nCan@00:44:41, 16.3C, 80% humidity, 14.6psia, 14.188V, 0.777A, 0.777A avg, 11.02W",Subject: "bac wcr sampling at most 2000ml" @04:13:28.04 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @04:13:28.05 Retry #6 of 10 after 10:00 @04:23:28.08 BACfans.email "Started stand-alone bac WCR in tube 2 at 00:47:53CEST13-Sep-19\n{1 => 13, 2 => 9}\nCan@00:44:41, 16.3C, 80% humidity, 14.6psia, 14.188V, 0.777A, 0.777A avg, 11.02W",Subject: "bac wcr sampling at most 2000ml" @04:23:28.11 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @04:23:28.12 Retry #7 of 10 after 10:00 @04:33:28.15 BACfans.email "Started stand-alone bac WCR in tube 2 at 00:47:53CEST13-Sep-19\n{1 => 13, 2 => 9}\nCan@00:44:41, 16.3C, 80% humidity, 14.6psia, 14.188V, 0.777A, 0.777A avg, 11.02W",Subject: "bac wcr sampling at most 2000ml" @04:33:28.18 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @04:33:28.19 Retry #8 of 10 after 10:00 @04:43:28.22 BACfans.email "Started stand-alone bac WCR in tube 2 at 00:47:53CEST13-Sep-19\n{1 => 13, 2 => 9}\nCan@00:44:41, 16.3C, 80% humidity, 14.6psia, 14.188V, 0.777A, 0.777A avg, 11.02W",Subject: "bac wcr sampling at most 2000ml" @04:43:28.25 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @04:43:28.26 Retry #9 of 10 after 10:00 @04:43:35.19
Powering core bus @04:43:39.91 Gate.power :raw,:ON @04:43:40.55 SPE.coast @04:43:40.62 SPE.configure SPEconfig @04:43:40.70 AS.configure ASconfig @04:43:40.80 SS.configure SSconfig @04:43:40.90 CC.configure CCconfig @04:43:40.99 CS.configure CSconfig @04:43:41.09 PC.configure PCconfig @04:43:41.18 PS.configure PSconfig @04:43:41.30 Forearm.configure LooseForearm @04:43:41.39 Elbow.configure LooseElbow @04:43:41.51 SC.configure SCloose @04:43:41.60 SE.configure SEconfig @04:43:41.64 CH.coast @04:43:41.69 CH.configure CHconfig @04:43:41.73 PH.coast @04:43:41.79 PH.configure PHconfig @04:43:41.82 PCR.off @04:43:41.85 PCR.pidtemp= 50,0.1,0,300,25,25 @04:43:41.89 PCR.caltemp= 2.8814,0.0372,0 @04:43:41.91 All dwarf controllers running firmware version 3.75 @04:43:42.03 Valve.reset @04:43:42.20 PCR.off @04:43:42.24 PCR.pidtemp= 50,0.1,0,300,25,25 @04:43:42.27 PCR.caltemp= 2.8814,0.0372,0 @04:43:42.45 Intake.select :close @04:43:42.49 Exhaust.select :close @04:43:42.50 CTV.dial :air @04:43:42.60 PSV.dial :air @04:43:42.62 CSV.dial :waste1 @04:43:42.84
Forearm.reconfigure LooseForearm @04:43:42.91 Forearm.home.seek :retract @04:43:42.93 PS.reconfigure PSconfig @04:43:43.03 PS.home.seek 0ml @04:43:43.31 SSV.dial :spit,avoiding: :exhaust @04:43:43.71 Hand.close @04:43:43.75
Elbow.jog 1500 @04:43:44.13 Slide::Error in rdyProcessing -- Processing Syringe overCurrent at 0.312ml @04:43:44.14 Waiting to Resume from Checkpoint "Processing Syringe overCurrent at 0.312ml" @04:43:44.18 CS.reconfigure CSfast @04:43:44.21 SS.reconfigure SSconfig @04:43:44.29 CS.home.seek 0ml @04:43:44.32 SS.home.seek 0ml @04:43:44.45
Elbow.reconfigure LooseElbow @04:43:44.49 Elbow.home.seek :clear @04:43:47.84 SE.reconfigure SEconfig @04:43:47.89 SE.home.seek :down @04:43:48.57 SC.reconfigure SCsloppy @04:43:48.61 SC.home.seek :initial @04:43:50.81 Forearm.seek :retract @04:43:50.89 Elbow.seek :hammer @04:43:52.21 Forearm.reconfigure TightForearm @04:43:52.26 Forearm.seek 5 + 350 counts @04:43:53.33 Forearm.seek :retract @04:43:54.43 CC.reconfigure CCconfig @04:43:54.44 PC.reconfigure PCconfig @04:43:54.55 PC.home.seek :open @04:43:54.56
CC.home.seek :open @04:53:28.29 BACfans.email "Started stand-alone bac WCR in tube 2 at 00:47:53CEST13-Sep-19\n{1 => 13, 2 => 9}\nCan@00:44:41, 16.3C, 80% humidity, 14.6psia, 14.188V, 0.777A, 0.777A avg, 11.02W",Subject: "bac wcr sampling at most 2000ml" @04:53:28.32 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @04:53:28.33 Retry #10 of 10 after 10:00 @05:03:28.36 BACfans.email "Started stand-alone bac WCR in tube 2 at 00:47:53CEST13-Sep-19\n{1 => 13, 2 => 9}\nCan@00:44:41, 16.3C, 80% humidity, 14.6psia, 14.188V, 0.777A, 0.777A avg, 11.02W",Subject: "bac wcr sampling at most 2000ml" @05:03:28.39 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @05:03:28.40 GoodNews.email "Then, in tube 2: wcrPhase2\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:134\n{1 => 14, 2 => 8}\nCan@02:34:44, 17.0C, 78% humidity, 14.6psia, 14.178V, 0.462A, 0.462A avg, 6.55W",Subject: "Asleep until 04:43" @05:03:28.43 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @05:03:28.44 Retry #1 of 10 after 10:00 @05:13:28.47 GoodNews.email "Then, in tube 2: wcrPhase2\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:134\n{1 => 14, 2 => 8}\nCan@02:34:44, 17.0C, 78% humidity, 14.6psia, 14.178V, 0.462A, 0.462A avg, 6.55W",Subject: "Asleep until 04:43" @05:13:28.50 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @05:13:28.51 Retry #2 of 10 after 10:00 @05:23:28.54 GoodNews.email "Then, in tube 2: wcrPhase2\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:134\n{1 => 14, 2 => 8}\nCan@02:34:44, 17.0C, 78% humidity, 14.6psia, 14.178V, 0.462A, 0.462A avg, 6.55W",Subject: "Asleep until 04:43" @05:23:28.57 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @05:23:28.58 Retry #3 of 10 after 10:00 @05:33:28.60 GoodNews.email "Then, in tube 2: wcrPhase2\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:134\n{1 => 14, 2 => 8}\nCan@02:34:44, 17.0C, 78% humidity, 14.6psia, 14.178V, 0.462A, 0.462A avg, 6.55W",Subject: "Asleep until 04:43" @05:33:28.63 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @05:33:28.64 Retry #4 of 10 after 10:00 @05:43:28.66 GoodNews.email "Then, in tube 2: wcrPhase2\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:134\n{1 => 14, 2 => 8}\nCan@02:34:44, 17.0C, 78% humidity, 14.6psia, 14.178V, 0.462A, 0.462A avg, 6.55W",Subject: "Asleep until 04:43" @05:43:28.69 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @05:43:28.70 Retry #5 of 10 after 10:00 @05:53:28.73 GoodNews.email "Then, in tube 2: wcrPhase2\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:134\n{1 => 14, 2 => 8}\nCan@02:34:44, 17.0C, 78% humidity, 14.6psia, 14.178V, 0.462A, 0.462A avg, 6.55W",Subject: "Asleep until 04:43" @05:53:28.76 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @05:53:28.77 Retry #6 of 10 after 10:00 @06:03:28.80 GoodNews.email "Then, in tube 2: wcrPhase2\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:134\n{1 => 14, 2 => 8}\nCan@02:34:44, 17.0C, 78% humidity, 14.6psia, 14.178V, 0.462A, 0.462A avg, 6.55W",Subject: "Asleep until 04:43" @06:03:28.83 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @06:03:28.84 Retry #7 of 10 after 10:00 @06:13:28.87 GoodNews.email "Then, in tube 2: wcrPhase2\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:134\n{1 => 14, 2 => 8}\nCan@02:34:44, 17.0C, 78% humidity, 14.6psia, 14.178V, 0.462A, 0.462A avg, 6.55W",Subject: "Asleep until 04:43" @06:13:28.90 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @06:13:28.91 Retry #8 of 10 after 10:00 @06:23:28.93 GoodNews.email "Then, in tube 2: wcrPhase2\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:134\n{1 => 14, 2 => 8}\nCan@02:34:44, 17.0C, 78% humidity, 14.6psia, 14.178V, 0.462A, 0.462A avg, 6.55W",Subject: "Asleep until 04:43" @06:23:28.96 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @06:23:28.97 Retry #9 of 10 after 10:00 @06:33:29.00 GoodNews.email "Then, in tube 2: wcrPhase2\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:134\n{1 => 14, 2 => 8}\nCan@02:34:44, 17.0C, 78% humidity, 14.6psia, 14.178V, 0.462A, 0.462A avg, 6.55W",Subject: "Asleep until 04:43" @06:33:29.03 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @06:33:29.04 Retry #10 of 10 after 10:00 @06:43:29.07 GoodNews.email "Then, in tube 2: wcrPhase2\n/home/esp/esp2/mission/TotaldeploymentFINAL3.rb:134\n{1 => 14, 2 => 8}\nCan@02:34:44, 17.0C, 78% humidity, 14.6psia, 14.178V, 0.462A, 0.462A avg, 6.55W",Subject: "Asleep until 04:43" @06:43:29.10 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @06:43:29.11 BadNews.email "Processing Syringe overCurrent at 0.312ml",Subject: "Slide::Error in rdyProcessing" @06:43:29.14 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @06:43:29.15 Retry #1 of 10 after 10:00 @06:53:29.18 BadNews.email "Processing Syringe overCurrent at 0.312ml",Subject: "Slide::Error in rdyProcessing" @06:53:29.21 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @06:53:29.22 Retry #2 of 10 after 10:00 @07:03:29.25 BadNews.email "Processing Syringe overCurrent at 0.312ml",Subject: "Slide::Error in rdyProcessing" @07:03:29.28 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @07:03:29.29 Retry #3 of 10 after 10:00 @07:13:29.32 BadNews.email "Processing Syringe overCurrent at 0.312ml",Subject: "Slide::Error in rdyProcessing" @07:13:29.35 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @07:13:29.36 Retry #4 of 10 after 10:00 @07:23:29.39 BadNews.email "Processing Syringe overCurrent at 0.312ml",Subject: "Slide::Error in rdyProcessing" @07:23:29.41 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @07:23:29.43 Retry #5 of 10 after 10:00 @07:33:29.45 BadNews.email "Processing Syringe overCurrent at 0.312ml",Subject: "Slide::Error in rdyProcessing" @07:33:29.48 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @07:33:29.49 Retry #6 of 10 after 10:00 @07:43:29.52 BadNews.email "Processing Syringe overCurrent at 0.312ml",Subject: "Slide::Error in rdyProcessing" @07:43:29.54 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @07:43:29.56 Retry #7 of 10 after 10:00 @07:53:29.58 BadNews.email "Processing Syringe overCurrent at 0.312ml",Subject: "Slide::Error in rdyProcessing" @07:53:29.61 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @07:53:29.62 Retry #8 of 10 after 10:00 @08:03:29.65 BadNews.email "Processing Syringe overCurrent at 0.312ml",Subject: "Slide::Error in rdyProcessing" @08:03:29.68 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @08:03:29.69 Retry #9 of 10 after 10:00 @08:13:29.72 BadNews.email "Processing Syringe overCurrent at 0.312ml",Subject: "Slide::Error in rdyProcessing" @08:13:29.75 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @08:13:29.76 Retry #10 of 10 after 10:00 @08:23:29.79 BadNews.email "Processing Syringe overCurrent at 0.312ml",Subject: "Slide::Error in rdyProcessing" @08:23:29.82 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @03:21:46.71CET29-Oct-19 I2C::Gateway::Reset in core_reader -- Received BREAK @15:59:11.59CET16-Mar-20
ESP2 v3.30 I2C Gateway v3.06 for core bus @15:59:14.65 Copyright 2018 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @15:59:15.17 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @15:59:22.91 Polling Can every 10:00 @15:59:25.26 SPE.coast @15:59:25.40 SPE.configure SPEconfig @15:59:25.53 AS.reconfigure ASconfig @15:59:25.58 AS.hold @15:59:25.65 AS.configure ASconfig @15:59:25.76 SS.reconfigure SSconfig @15:59:25.81 SS.hold @15:59:25.88 SS.configure SSconfig @15:59:25.99 CC.reconfigure CCconfig @15:59:26.04 CC.hold @15:59:26.10 CC.configure CCconfig @15:59:26.27 CS.reconfigure CSconfig @15:59:26.37 CS.hold @15:59:26.51 CS.configure CSconfig @15:59:27.40 PC.reconfigure PCconfig @15:59:27.44 PC.hold @15:59:27.52 PC.configure PCconfig @15:59:27.64 PS.configure PSconfig @15:59:27.76 Forearm.reconfigure LooseForearm @15:59:27.82 Forearm.hold @15:59:27.89 Forearm.configure LooseForearm @15:59:28.06 Elbow.reconfigure LooseElbow @15:59:28.17 Elbow.hold @15:59:28.28 Elbow.configure LooseElbow @15:59:28.55 SC.reconfigure SCloose @15:59:28.64 SC.hold @15:59:28.78 SC.configure SCloose @15:59:29.00 SE.reconfigure SEconfig @15:59:29.07 SE.hold @15:59:29.13 SE.configure SEconfig @15:59:29.18 CH.coast @15:59:29.25 CH.configure CHconfig @15:59:29.28 PH.coast @15:59:29.35 PH.configure PHconfig @15:59:29.40 PCR.off @15:59:29.43 PCR.pidtemp= 50,0.1,0,300,25,25 @15:59:29.46 PCR.caltemp= 2.8814,0.0372,0 @15:59:29.49 All dwarf controllers running firmware version 3.75 @15:59:29.67 Cleanly exited at 15:58:03CET16-Mar-20 @15:59:29.73 Loading /home/esp/esp2/mission/stdMER2017.rb mission @15:59:30.96 Session Begun @15:59:31.34
AFV.select :carrierSolution @15:59:31.48 delay 2 seconds @15:59:33.52 AS.seek 0.5ml @16:00:02.66 delay 1 second @16:00:03.69 AFV.select :holdingCoil @16:00:03.81 ATV.dial :extSrc @16:00:05.34 AS.reconfigure ASslow1 @16:00:05.42 AS.seek 0.53ml @16:00:11.73 delay 5 seconds @16:00:16.83 AS.reconfigure ASslow2 @16:00:16.90 AS.seek 0.54ml @16:00:27.15 delay 20 seconds @16:00:47.25 AS.reconfigure ASslow1 @16:00:47.33 AS.seek 0.57ml @16:00:53.63 delay 5 seconds @16:00:58.74 AS.reconfigure ASslow2 @16:00:58.82 AS.seek 0.58ml @16:01:09.13 delay 20 seconds @16:01:29.22 AS.reconfigure ASslow1 @16:01:29.30 AS.seek 0.61ml @16:01:35.68 delay 5 seconds @16:01:40.78 AS.reconfigure ASslow2 @16:01:40.85 AS.seek 0.62ml @16:01:51.15 delay 20 seconds @16:02:11.25 AS.reconfigure ASslow1 @16:02:11.33 AS.seek 0.65ml @16:02:17.63 delay 5 seconds @16:02:22.73 AS.reconfigure ASslow2 @16:02:22.81 AS.seek 0.66ml @16:02:33.29 delay 20 seconds @16:02:53.34 AS.reconfigure ASslow1 @16:02:53.41 AS.seek 0.705ml @16:03:02.77 delay 10 seconds @16:03:12.81 ATV.dial :air1 @16:03:14.57 AS.seek 0.725ml @16:03:18.90 delay 3 seconds @16:03:21.93 ATV.dial :waste @16:03:22.52 AS.reconfigure ASslow0 @16:03:22.59 AS.seek :empty @16:04:36.09 Setting up PCR for CG -- writing /var/log/esp/CG20mar1616h.pcr @16:04:36.12 Building PCR Slug from extSrc with MM1 mix, PP2 primer @16:04:36.16 Priming with carrier @16:04:36.18 ATV.dial ARV @16:04:37.71 ARV.dial :carrier @16:04:38.10 AS.reconfigure ASnorm @16:04:38.19 AS.seek 0.499ml @16:05:03.71 AFV.select :carrierSolution @16:05:03.85 delay 2 seconds @16:05:05.89 AS.reconfigure ASconfig @16:05:05.97 AS.seek 1ml @16:05:35.05 delay 1 second @16:05:36.08 AFV.select :holdingCoil @16:05:36.22 delay 1 second @16:05:37.25 ATV.dial :waste @16:05:38.97 AS.reconfigure ASnorm @16:05:39.05 AS.seek :empty @16:06:29.80 AFV.select :carrierSolution @16:06:29.94 delay 2 seconds @16:06:31.98 AS.reconfigure ASconfig @16:06:32.05 AS.seek 0.5ml @16:07:01.15 delay 1 second @16:07:02.18 AFV.select :holdingCoil @16:07:02.30 Building Slug @16:07:02.32 ATV.dial :ARV @16:07:04.14 delay 2 seconds @16:07:06.17 ARV.dial :air1 @16:07:06.60 delay 2 seconds @16:07:08.70 AS.reconfigure ASslow2 @16:07:08.78 AS.seek 0.506ml @16:07:15.23 delay 2 seconds @16:07:17.26 ARV.dial :MM1 @16:07:17.88 delay 2 seconds @16:07:19.91 Adding MM1 @16:07:20.02 AS.seek 0.524ml @16:07:38.40 delay 25 seconds @16:08:03.43 ARV.dial :PP2 @16:08:03.86 Adding PP2 @16:08:03.88 delay 2 seconds @16:08:05.99 AS.seek 0.53ml @16:08:12.18 delay 10 seconds @16:08:22.22 ARV.dial :air2 @16:08:22.64 adding ARV air @16:08:22.66 delay 2 seconds @16:08:24.77 AS.seek 0.536ml @16:08:31.40 delay 5 seconds @16:08:36.43 ARV.dial :carrier @16:08:37.74 delay 7 seconds @16:08:44.85 AS.seek 0.594ml @16:09:43.45 delay 5 seconds @16:09:48.48 ATV.dial :extSrc @16:09:49.07 delay 2 seconds @16:09:51.17 AS.seek 0.6ml @16:09:57.71 delay 4 seconds @16:10:01.74 ATV.dial ARV @16:10:02.31 delay 2 seconds @16:10:04.34 ARV.dial :carrier @16:10:04.36 delay 2 seconds @16:10:06.48 AS.seek 0.65ml @16:10:57.26 delay 4 seconds @16:11:01.29 ATV.dial PCR @16:11:02.07 ASV.dial :waste1 @16:11:02.70 ARV.dial :air1 @16:11:03.14 Positioning Slug by 0.324ml @16:11:03.26 AS.seek 0.326ml @16:16:29.37 delay 5 seconds @16:16:34.40 ATV.dial ARV @16:16:35.21 ASV.dial :PCRlock @16:16:35.64 delay 5 seconds @16:16:40.68 Running CG Profile60C300 thermocycling @16:16:40.69 PCR.program Profile60C300 @16:16:40.85 Profile60C300 should complete in < 1:49:17 @16:16:40.87 PCR.run 2:21:36.25 @17:39:22.67 PCR.off @17:39:24.81 PCR for CG completed -- closed /var/log/esp/CG20mar1616h.pcr @17:39:24.91 Cleaning PCR @17:39:24.93 AFV.select :carrierSolution @17:39:25.07 delay 2 seconds @17:39:27.11 AS.reconfigure ASconfig @17:39:27.18 AS.seek 0.5ml @17:39:37.48 delay 1 second @17:39:38.51 AFV.select :holdingCoil @17:39:38.64 ASV.dial :waste1 @17:39:39.07 ATV.dial :air3 @17:39:40.42 AS.reconfigure ASslow1 @17:39:40.50 AS.seek 0.51ml @17:39:42.85 delay 2 seconds @17:39:44.88 ATV.dial :bleach @17:39:45.56 AS.seek 0.81ml @17:40:46.17 delay 3 seconds @17:40:49.20 ATV.dial :air3 @17:40:49.79 delay 3 seconds @17:40:52.89 AS.seek 0.835ml @17:40:58.23 delay 3 seconds @17:41:01.26 ATV.dial :bleach @17:41:01.93 AS.seek 0.82ml @17:41:05.82 delay 3 seconds @17:41:08.85 ARV.dial :waste @17:41:09.50 delay 3 seconds @17:41:12.52 ATV.dial :ARV @17:41:13.46 AS.reconfigure ASconfig @17:41:13.54 AS.seek 0.47ml @17:41:33.99 AFV.select :carrierSolution @17:41:34.13 delay 2 seconds @17:41:36.17 AS.seek 1ml @17:42:06.96 delay 1 second @17:42:07.99 AFV.select :holdingCoil @17:42:08.11 delay 1 second @17:42:09.15 AS.reconfigure ASfast @17:42:09.22 AS.seek 0ml @17:42:36.96 AFV.select :carrierSolution @17:42:37.10 delay 2 seconds @17:42:39.14 AS.reconfigure ASconfig @17:42:39.21 AS.seek 1ml @17:43:37.06 delay 1 second @17:43:38.09 AFV.select :holdingCoil @17:43:38.21 delay 1 second @17:43:39.25 AS.reconfigure ASfast @17:43:39.32 AS.seek 0ml @17:44:07.12 AFV.select :carrierSolution @17:44:07.26 delay 2 seconds @17:44:09.30 AS.reconfigure ASconfig @17:44:09.37 AS.seek 1ml @17:45:07.15 delay 1 second @17:45:08.18 AFV.select :holdingCoil @17:45:08.32 delay 1 second @17:45:09.36 AS.reconfigure ASfast @17:45:09.43 AS.seek 0ml @17:45:37.21 ARV.dial between carrier and air1 @17:45:37.85 ATV.dial :air3 @17:45:39.20 AS.reconfigure ASslow1 @17:45:39.28 AS.seek 0.01ml @17:45:41.74 delay 2 seconds @17:45:43.77 ATV.dial :bleach @17:45:44.45 AS.seek 0.81ml @17:48:25.68 delay 3 seconds @17:48:28.71 ATV.dial :air3 @17:48:29.31 delay 3 seconds @17:48:32.45 AS.seek 0.835ml @17:48:37.80 delay 3 seconds @17:48:40.82 ATV.dial :bleach @17:48:41.50 AS.seek 0.82ml @17:48:44.84 delay 3 seconds @17:48:47.87 ATV.dial PCR @17:48:48.30 AS.reconfigure ASconfig @17:48:48.38 AS.seek :empty @17:49:35.85 PCR.program :bleachProfile @17:49:35.92 PCR program should complete in < 2:45 @17:49:35.94 PCR.run 10:00 @17:52:34.76 PCR.off @17:52:34.81 AFV.select :carrierSolution @17:52:34.94 delay 2 seconds @17:52:36.98 AS.seek 1ml @17:53:34.84 delay 1 second @17:53:35.87 AFV.select :holdingCoil @17:53:35.99 delay 1 second @17:53:37.03 AS.reconfigure ASfast @17:53:37.10 AS.seek 0ml @17:54:04.92 delay 3 seconds @17:54:07.95 AFV.select :carrierSolution @17:54:08.07 delay 2 seconds @17:54:10.11 AS.reconfigure ASconfig @17:54:10.18 AS.seek 1ml @17:55:08.01 delay 1 second @17:55:09.04 AFV.select :holdingCoil @17:55:09.16 delay 1 second @17:55:10.20 AS.reconfigure ASfast @17:55:10.27 AS.seek 0ml @17:55:38.04 delay 3 seconds @17:55:41.07 AFV.select :carrierSolution @17:55:41.19 delay 2 seconds @17:55:43.23 AS.reconfigure ASconfig @17:55:43.31 AS.seek 1ml @17:56:41.10 delay 1 second @17:56:42.13 AFV.select :holdingCoil @17:56:42.25 delay 1 second @17:56:43.29 AS.reconfigure ASfast @17:56:43.36 AS.seek 0ml @17:57:11.04 delay 3 seconds @17:57:14.07 AFV.select :carrierSolution @17:57:14.19 delay 2 seconds @17:57:16.23 AS.reconfigure ASconfig @17:57:16.31 AS.seek 1ml @17:58:14.10 delay 1 second @17:58:15.13 AFV.select :holdingCoil @17:58:15.28 delay 1 second @17:58:16.32 AS.reconfigure ASfast @17:58:16.39 AS.seek 0ml @17:58:44.32 delay 3 seconds @17:58:47.35 AFV.select :carrierSolution @17:58:47.47 delay 2 seconds @17:58:49.51 AS.reconfigure ASconfig @17:58:49.59 AS.seek 1ml @17:59:47.35 delay 1 second @17:59:48.38 AFV.select :holdingCoil @17:59:48.49 delay 1 second @17:59:49.53 AS.reconfigure ASnorm @17:59:49.60 AS.seek 0ml @18:00:40.34
AFV.select :carrierSolution @18:00:40.47 delay 2 seconds @18:00:42.51 AS.reconfigure ASconfig @18:00:42.58 AS.seek 0.5ml @18:01:11.65 delay 1 second @18:01:12.68 AFV.select :holdingCoil @18:01:12.80 ATV.dial :air1 @18:01:14.34 AS.reconfigure ASslow2 @18:01:14.41 AS.seek 0.506ml @18:01:20.67 ATV.dial :extSrc @18:01:22.42 AS.seek 0.512ml @18:01:28.82 delay 5 seconds @18:01:33.85 ATV.dial :air1 @18:01:35.64 AS.reconfigure ASslow1 @18:01:35.71 AS.seek 0.542ml @18:01:42.04 ATV.dial :waste @18:01:42.63 AS.reconfigure ASconfig @18:01:42.70 AS.seek :empty @18:02:14.43 Setting up PCR for CG -- writing /var/log/esp/CG20mar1618h.pcr @18:02:14.46 Building PCR Slug from extSrc with MM1 mix, PP2 primer @18:02:14.50 Priming with carrier @18:02:14.53 ATV.dial ARV @18:02:16.06 ARV.dial :carrier @18:02:16.45 AS.reconfigure ASnorm @18:02:16.53 AS.seek 0.499ml @18:02:42.15 AFV.select :carrierSolution @18:02:42.29 delay 2 seconds @18:02:44.33 AS.reconfigure ASconfig @18:02:44.41 AS.seek 1ml @18:03:13.48 delay 1 second @18:03:14.51 AFV.select :holdingCoil @18:03:14.64 delay 1 second @18:03:15.67 ATV.dial :waste @18:03:17.36 AS.reconfigure ASnorm @18:03:17.44 AS.seek :empty @18:04:08.74 AFV.select :carrierSolution @18:04:08.88 delay 2 seconds @18:04:10.92 AS.reconfigure ASconfig @18:04:10.99 AS.seek 0.5ml @18:04:40.10 delay 1 second @18:04:41.13 AFV.select :holdingCoil @18:04:41.25 Building Slug @18:04:41.27 ATV.dial :ARV @18:04:42.97 delay 2 seconds @18:04:44.99 ARV.dial :air1 @18:04:45.44 delay 2 seconds @18:04:47.53 AS.reconfigure ASslow2 @18:04:47.61 AS.seek 0.506ml @18:04:53.87 delay 2 seconds @18:04:55.90 ARV.dial :MM1 @18:04:56.61 delay 2 seconds @18:04:58.63 Adding MM1 @18:04:58.74 AS.seek 0.524ml @18:05:17.26 delay 25 seconds @18:05:42.29 ARV.dial :PP2 @18:05:42.72 Adding PP2 @18:05:42.73 delay 2 seconds @18:05:44.85 AS.seek 0.53ml @18:05:51.28 delay 10 seconds @18:06:01.32 ARV.dial :air2 @18:06:01.74 adding ARV air @18:06:01.75 delay 2 seconds @18:06:03.87 AS.seek 0.536ml @18:06:10.21 delay 5 seconds @18:06:15.24 ARV.dial :carrier @18:06:16.54 delay 7 seconds @18:06:23.65 AS.seek 0.594ml @18:07:22.43 delay 5 seconds @18:07:27.46 ATV.dial :extSrc @18:07:28.07 delay 2 seconds @18:07:30.16 AS.seek 0.6ml @18:07:36.34 delay 4 seconds @18:07:40.37 ATV.dial ARV @18:07:40.95 delay 2 seconds @18:07:42.98 ARV.dial :carrier @18:07:43.00 delay 2 seconds @18:07:45.10 AS.seek 0.65ml @18:08:35.73 delay 4 seconds @18:08:39.76 ATV.dial PCR @18:08:40.56 ASV.dial :waste1 @18:08:40.58 ARV.dial :air1 @18:08:41.13 Positioning Slug by 0.324ml @18:08:41.25 AS.seek 0.326ml @18:14:07.29 delay 5 seconds @18:14:12.32 ATV.dial ARV @18:14:13.14 ASV.dial :PCRlock @18:14:13.58 delay 5 seconds @18:14:18.61 Running CG Profile60C300 thermocycling @18:14:18.63 PCR.program Profile60C300 @18:14:18.79 Profile60C300 should complete in < 1:49:17 @18:14:18.81 PCR.run 2:21:36.25 @19:36:43.12 PCR.off @19:36:45.43 PCR for CG completed -- closed /var/log/esp/CG20mar1618h.pcr @19:36:45.53 Cleaning PCR @19:36:45.55 AFV.select :carrierSolution @19:36:45.69 delay 2 seconds @19:36:47.73 AS.reconfigure ASconfig @19:36:47.80 AS.seek 0.5ml @19:36:58.14 delay 1 second @19:36:59.17 AFV.select :holdingCoil @19:36:59.30 ASV.dial :waste1 @19:36:59.72 ATV.dial :air3 @19:37:01.08 AS.reconfigure ASslow1 @19:37:01.16 AS.seek 0.51ml @19:37:03.46 delay 2 seconds @19:37:05.49 ATV.dial :bleach @19:37:06.16 AS.seek 0.81ml @19:38:06.79 delay 3 seconds @19:38:09.82 ATV.dial :air3 @19:38:10.42 delay 3 seconds @19:38:13.52 AS.seek 0.835ml @19:38:18.85 delay 3 seconds @19:38:21.88 ATV.dial :bleach @19:38:22.56 AS.seek 0.82ml @19:38:25.90 delay 3 seconds @19:38:28.93 ARV.dial :waste @19:38:29.56 delay 3 seconds @19:38:32.59 ATV.dial :ARV @19:38:33.53 AS.reconfigure ASconfig @19:38:33.60 AS.seek 0.47ml @19:38:54.03 AFV.select :carrierSolution @19:38:54.17 delay 2 seconds @19:38:56.21 AS.seek 1ml @19:39:26.98 delay 1 second @19:39:28.01 AFV.select :holdingCoil @19:39:28.14 delay 1 second @19:39:29.18 AS.reconfigure ASfast @19:39:29.25 AS.seek 0ml @19:39:57.12 AFV.select :carrierSolution @19:39:57.26 delay 2 seconds @19:39:59.30 AS.reconfigure ASconfig @19:39:59.37 AS.seek 1ml @19:40:57.14 delay 1 second @19:40:58.17 AFV.select :holdingCoil @19:40:58.29 delay 1 second @19:40:59.33 AS.reconfigure ASfast @19:40:59.40 AS.seek 0ml @19:41:27.17 AFV.select :carrierSolution @19:41:27.31 delay 2 seconds @19:41:29.35 AS.reconfigure ASconfig @19:41:29.42 AS.seek 1ml @19:42:27.20 delay 1 second @19:42:28.23 AFV.select :holdingCoil @19:42:28.35 delay 1 second @19:42:29.39 AS.reconfigure ASfast @19:42:29.46 AS.seek 0ml @19:42:57.31 ARV.dial between carrier and air1 @19:42:57.85 ATV.dial :air3 @19:42:59.19 AS.reconfigure ASslow1 @19:42:59.27 AS.seek 0.01ml @19:43:01.59 delay 2 seconds @19:43:03.62 ATV.dial :bleach @19:43:04.30 AS.seek 0.81ml @19:45:45.47 delay 3 seconds @19:45:48.50 ATV.dial :air3 @19:45:49.09 delay 3 seconds @19:45:52.19 AS.seek 0.835ml @19:45:57.49 delay 3 seconds @19:46:00.52 ATV.dial :bleach @19:46:01.19 AS.seek 0.82ml @19:46:04.51 delay 3 seconds @19:46:07.54 ATV.dial PCR @19:46:07.98 AS.reconfigure ASconfig @19:46:08.05 AS.seek :empty @19:46:55.53 PCR.program :bleachProfile @19:46:55.61 PCR program should complete in < 2:45 @19:46:55.63 PCR.run 10:00 @19:49:54.46 PCR.off @19:49:54.51 AFV.select :carrierSolution @19:49:54.64 delay 2 seconds @19:49:56.68 AS.seek 1ml @19:50:54.53 delay 1 second @19:50:55.56 AFV.select :holdingCoil @19:50:55.70 delay 1 second @19:50:56.74 AS.reconfigure ASfast @19:50:56.81 AS.seek 0ml @19:51:24.73 delay 3 seconds @19:51:27.76 AFV.select :carrierSolution @19:51:27.88 delay 2 seconds @19:51:29.92 AS.reconfigure ASconfig @19:51:30.00 AS.seek 1ml @19:52:27.84 delay 1 second @19:52:28.87 AFV.select :holdingCoil @19:52:28.99 delay 1 second @19:52:30.03 AS.reconfigure ASfast @19:52:30.10 AS.seek 0ml @19:52:57.86 delay 3 seconds @19:53:00.89 AFV.select :carrierSolution @19:53:01.01 delay 2 seconds @19:53:03.05 AS.reconfigure ASconfig @19:53:03.12 AS.seek 1ml @19:54:00.89 delay 1 second @19:54:01.92 AFV.select :holdingCoil @19:54:02.04 delay 1 second @19:54:03.08 AS.reconfigure ASfast @19:54:03.15 AS.seek 0ml @19:54:30.78 delay 3 seconds @19:54:33.81 AFV.select :carrierSolution @19:54:33.93 delay 2 seconds @19:54:35.97 AS.reconfigure ASconfig @19:54:36.04 AS.seek 1ml @19:55:33.81 delay 1 second @19:55:34.84 AFV.select :holdingCoil @19:55:34.96 delay 1 second @19:55:36.00 AS.reconfigure ASfast @19:55:36.07 AS.seek 0ml @19:56:03.69 delay 3 seconds @19:56:06.72 AFV.select :carrierSolution @19:56:06.84 delay 2 seconds @19:56:08.88 AS.reconfigure ASconfig @19:56:08.96 AS.seek 1ml @19:57:06.76 delay 1 second @19:57:07.79 AFV.select :holdingCoil @19:57:07.91 delay 1 second @19:57:08.95 AS.reconfigure ASnorm @19:57:09.02 AS.seek 0ml @19:57:59.77
AFV.select :carrierSolution @19:57:59.90 delay 2 seconds @19:58:01.94 AS.reconfigure ASconfig @19:58:02.52 AS.seek 0.5ml @19:58:31.56 delay 1 second @19:58:32.59 AFV.select :holdingCoil @19:58:32.70 ATV.dial :air1 @19:58:34.25 AS.reconfigure ASslow2 @19:58:34.33 AS.seek 0.51ml @19:58:44.59 delay 5 seconds @19:58:49.62 ATV.dial :extSrc @19:58:51.39 AS.seek 0.53ml @19:59:11.76 delay 10 seconds @19:59:21.80 ATV.dial :air1 @19:59:23.61 AS.reconfigure ASslow1 @19:59:23.86 AS.seek 0.56ml @19:59:30.33 ATV.dial :waste @19:59:31.03 AS.reconfigure ASconfig @19:59:31.17 AS.seek :empty @20:00:03.96 Setting up PCR for CG -- writing /var/log/esp/CG20mar1620h.pcr @20:00:03.99 Building PCR Slug from extSrc with MM1 mix, PP2 primer @20:00:04.03 Priming with carrier @20:00:04.05 ATV.dial ARV @20:00:05.59 ARV.dial :carrier @20:00:05.97 AS.reconfigure ASnorm @20:00:06.05 AS.seek 0.499ml @20:00:31.65 AFV.select :carrierSolution @20:00:31.79 delay 2 seconds @20:00:33.83 AS.reconfigure ASconfig @20:00:33.90 AS.seek 1ml @20:01:03.00 delay 1 second @20:01:04.03 AFV.select :holdingCoil @20:01:04.17 delay 1 second @20:01:05.20 ATV.dial :waste @20:01:06.91 AS.reconfigure ASnorm @20:01:07.00 AS.seek :empty @20:01:57.73 AFV.select :carrierSolution @20:01:57.87 delay 2 seconds @20:01:59.91 AS.reconfigure ASconfig @20:01:59.98 AS.seek 0.5ml @20:02:29.12 delay 1 second @20:02:30.15 AFV.select :holdingCoil @20:02:30.28 Building Slug @20:02:30.30 ATV.dial :ARV @20:02:32.10 delay 2 seconds @20:02:34.12 ARV.dial :air1 @20:02:34.55 delay 2 seconds @20:02:36.64 AS.reconfigure ASslow2 @20:02:36.72 AS.seek 0.506ml @20:02:43.23 delay 2 seconds @20:02:45.26 ARV.dial :MM1 @20:02:45.89 delay 2 seconds @20:02:47.92 Adding MM1 @20:02:48.03 AS.seek 0.524ml @20:03:06.45 delay 25 seconds @20:03:31.48 ARV.dial :PP2 @20:03:31.92 Adding PP2 @20:03:31.93 delay 2 seconds @20:03:34.04 AS.seek 0.53ml @20:03:40.58 delay 10 seconds @20:03:50.62 ARV.dial :air2 @20:03:51.12 adding ARV air @20:03:51.15 delay 2 seconds @20:03:53.34 AS.seek 0.536ml @20:03:59.73 delay 5 seconds @20:04:04.77 ARV.dial :carrier @20:04:06.06 delay 7 seconds @20:04:13.17 AS.seek 0.594ml @20:05:11.70 delay 5 seconds @20:05:16.73 ATV.dial :extSrc @20:05:17.33 delay 2 seconds @20:05:19.43 AS.seek 0.6ml @20:05:25.70 delay 4 seconds @20:05:29.73 ATV.dial ARV @20:05:30.31 delay 2 seconds @20:05:32.34 ARV.dial :carrier @20:05:32.36 delay 2 seconds @20:05:34.48 AS.seek 0.65ml @20:06:25.14 delay 4 seconds @20:06:29.17 ATV.dial PCR @20:06:29.97 ASV.dial :waste1 @20:06:29.99 ARV.dial :air1 @20:06:30.44 Positioning Slug by 0.324ml @20:06:30.56 AS.seek 0.326ml @20:11:56.62 delay 5 seconds @20:12:01.65 ATV.dial ARV @20:12:02.45 ASV.dial :PCRlock @20:12:02.88 delay 5 seconds @20:12:07.91 Running CG Profile60C300 thermocycling @20:12:07.93 PCR.program Profile60C300 @20:12:08.09 Profile60C300 should complete in < 1:49:17 @20:12:08.11 PCR.run 2:21:36.25 @21:34:21.16 PCR.off @21:34:23.18 PCR for CG completed -- closed /var/log/esp/CG20mar1620h.pcr @21:34:23.26 Cleaning PCR @21:34:23.27 AFV.select :carrierSolution @21:34:23.39 delay 2 seconds @21:34:25.41 AS.reconfigure ASconfig @21:34:25.47 AS.seek 0.5ml @21:34:35.74 delay 1 second @21:34:36.77 AFV.select :holdingCoil @21:34:36.87 ASV.dial :waste1 @21:34:37.27 ATV.dial :air3 @21:34:38.57 AS.reconfigure ASslow1 @21:34:38.63 AS.seek 0.51ml @21:34:40.92 delay 2 seconds @21:34:42.95 ATV.dial :bleach @21:34:43.59 AS.seek 0.81ml @21:35:44.18 delay 3 seconds @21:35:47.21 ATV.dial :air3 @21:35:47.77 delay 3 seconds @21:35:50.84 AS.seek 0.835ml @21:35:56.12 delay 3 seconds @21:35:59.15 ATV.dial :bleach @21:35:59.76 AS.seek 0.82ml @21:36:03.09 delay 3 seconds @21:36:06.12 ARV.dial :waste @21:36:06.74 delay 3 seconds @21:36:09.76 ATV.dial :ARV @21:36:10.65 AS.reconfigure ASconfig @21:36:10.71 AS.seek 0.47ml @21:36:31.13 AFV.select :carrierSolution @21:36:31.25 delay 2 seconds @21:36:33.65 AS.seek 1ml @21:37:04.38 delay 1 second @21:37:05.41 AFV.select :holdingCoil @21:37:05.51 delay 1 second @21:37:06.53 AS.reconfigure ASfast @21:37:06.59 AS.seek 0ml @21:37:34.49 AFV.select :carrierSolution @21:37:34.61 delay 2 seconds @21:37:36.63 AS.reconfigure ASconfig @21:37:36.69 AS.seek 1ml @21:38:34.44 delay 1 second @21:38:35.46 AFV.select :holdingCoil @21:38:35.56 delay 1 second @21:38:36.58 AS.reconfigure ASfast @21:38:36.64 AS.seek 0ml @21:39:04.41 AFV.select :carrierSolution @21:39:04.53 delay 2 seconds @21:39:06.55 AS.reconfigure ASconfig @21:39:06.61 AS.seek 1ml @21:40:04.35 delay 1 second @21:40:05.38 AFV.select :holdingCoil @21:40:05.48 delay 1 second @21:40:06.50 AS.reconfigure ASfast @21:40:06.56 AS.seek 0ml @21:40:34.48 ARV.dial between carrier and air1 @21:40:34.99 ATV.dial :air3 @21:40:36.30 AS.reconfigure ASslow1 @21:40:36.36 AS.seek 0.01ml @21:40:38.76 delay 2 seconds @21:40:40.79 ATV.dial :bleach @21:40:41.42 AS.seek 0.81ml @21:43:22.59 delay 3 seconds @21:43:25.62 ATV.dial :air3 @21:43:26.19 delay 3 seconds @21:43:29.26 AS.seek 0.835ml @21:43:34.58 delay 3 seconds @21:43:37.60 ATV.dial :bleach @21:43:38.23 AS.seek 0.82ml @21:43:41.54 delay 3 seconds @21:43:44.57 ATV.dial PCR @21:43:44.97 AS.reconfigure ASconfig @21:43:45.03 AS.seek :empty @21:44:32.48 PCR.program :bleachProfile @21:44:32.54 PCR program should complete in < 2:45 @21:44:32.55 PCR.run 10:00 @21:47:31.36 PCR.off @21:47:31.39 AFV.select :carrierSolution @21:47:31.50 delay 2 seconds @21:47:33.52 AS.seek 1ml @21:48:31.34 delay 1 second @21:48:32.37 AFV.select :holdingCoil @21:48:32.47 delay 1 second @21:48:33.49 AS.reconfigure ASfast @21:48:33.55 AS.seek 0ml @21:49:01.13 delay 3 seconds @21:49:04.16 AFV.select :carrierSolution @21:49:04.26 delay 2 seconds @21:49:06.28 AS.reconfigure ASconfig @21:49:06.35 AS.seek 1ml @21:50:04.09 delay 1 second @21:50:05.12 AFV.select :holdingCoil @21:50:05.22 delay 1 second @21:50:06.24 AS.reconfigure ASfast @21:50:06.30 AS.seek 0ml @21:50:33.96 delay 3 seconds @21:50:36.99 AFV.select :carrierSolution @21:50:37.09 delay 2 seconds @21:50:39.11 AS.reconfigure ASconfig @21:50:39.18 AS.seek 1ml @21:51:36.95 delay 1 second @21:51:37.98 AFV.select :holdingCoil @21:51:38.08 delay 1 second @21:51:39.10 AS.reconfigure ASfast @21:51:39.16 AS.seek 0ml @21:52:06.82 delay 3 seconds @21:52:09.85 AFV.select :carrierSolution @21:52:09.95 delay 2 seconds @21:52:11.97 AS.reconfigure ASconfig @21:52:12.03 AS.seek 1ml @21:53:09.77 delay 1 second @21:53:10.80 AFV.select :holdingCoil @21:53:10.90 delay 1 second @21:53:11.92 AS.reconfigure ASfast @21:53:11.99 AS.seek 0ml @21:53:39.65 delay 3 seconds @21:53:42.68 AFV.select :carrierSolution @21:53:42.78 delay 2 seconds @21:53:44.80 AS.reconfigure ASconfig @21:53:44.87 AS.seek 1ml @21:54:42.62 delay 1 second @21:54:43.65 AFV.select :holdingCoil @21:54:43.75 delay 1 second @21:54:44.77 AS.reconfigure ASnorm @21:54:44.83 AS.seek 0ml @21:55:35.53
AFV.select :carrierSolution @21:55:35.64 delay 2 seconds @21:55:37.66 AS.reconfigure ASconfig @21:55:37.72 AS.seek 0.5ml @21:56:06.74 delay 1 second @21:56:07.77 AFV.select :holdingCoil @21:56:07.88 ATV.dial :air1 @21:56:09.38 AS.reconfigure ASslow2 @21:56:09.44 AS.seek 0.506ml @21:56:15.94 ATV.dial :extSrc @21:56:17.65 AS.seek 0.512ml @21:56:24.31 delay 5 seconds @21:56:29.34 ATV.dial :air1 @21:56:31.07 AS.reconfigure ASslow1 @21:56:31.13 AS.seek 0.542ml @21:56:37.41 ATV.dial :waste @21:56:37.97 AS.reconfigure ASconfig @21:56:38.03 AS.seek :empty @21:57:09.66 Setting up PCR for CG -- writing /var/log/esp/CG20mar1621h.pcr @21:57:09.68 Building PCR Slug from extSrc with MM1 mix, PP2 primer @21:57:09.71 Priming with carrier @21:57:09.73 ATV.dial ARV @21:57:11.24 ARV.dial :carrier @21:57:11.59 AS.reconfigure ASnorm @21:57:11.65 AS.seek 0.499ml @21:57:37.21 AFV.select :carrierSolution @21:57:37.34 delay 2 seconds @21:57:39.36 AS.reconfigure ASconfig @21:57:39.43 AS.seek 1ml @21:58:08.50 delay 1 second @21:58:09.52 AFV.select :holdingCoil @21:58:09.62 delay 1 second @21:58:10.64 ATV.dial :waste @21:58:12.33 AS.reconfigure ASnorm @21:58:12.39 AS.seek :empty @21:59:03.15 AFV.select :carrierSolution @21:59:03.27 delay 2 seconds @21:59:05.29 AS.reconfigure ASconfig @21:59:05.36 AS.seek 0.5ml @21:59:34.47 delay 1 second @21:59:35.49 AFV.select :holdingCoil @21:59:35.59 Building Slug @21:59:35.60 ATV.dial :ARV @21:59:37.27 delay 2 seconds @21:59:39.29 ARV.dial :air1 @21:59:39.69 delay 2 seconds @21:59:41.76 AS.reconfigure ASslow2 @21:59:41.82 AS.seek 0.506ml @21:59:48.00 delay 2 seconds @21:59:50.02 ARV.dial :MM1 @21:59:50.63 delay 2 seconds @21:59:52.65 Adding MM1 @21:59:52.73 AS.seek 0.524ml @22:00:11.02 delay 25 seconds @22:00:36.05 ARV.dial :PP2 @22:00:36.46 Adding PP2 @22:00:36.47 delay 2 seconds @22:00:38.55 AS.seek 0.53ml @22:00:44.82 delay 10 seconds @22:00:54.86 ARV.dial :air2 @22:00:55.25 adding ARV air @22:00:55.26 delay 2 seconds @22:00:57.34 AS.seek 0.536ml @22:01:03.51 delay 5 seconds @22:01:08.54 ARV.dial :carrier @22:01:09.83 delay 7 seconds @22:01:16.91 AS.seek 0.594ml @22:02:15.46 delay 5 seconds @22:02:20.49 ATV.dial :extSrc @22:02:21.07 delay 2 seconds @22:02:23.14 AS.seek 0.6ml @22:02:29.59 delay 4 seconds @22:02:33.62 ATV.dial ARV @22:02:34.19 delay 2 seconds @22:02:36.21 ARV.dial :carrier @22:02:36.22 delay 2 seconds @22:02:38.30 AS.seek 0.65ml @22:03:28.82 delay 4 seconds @22:03:32.85 ATV.dial PCR @22:03:33.62 ASV.dial :waste1 @22:03:33.64 ARV.dial :air1 @22:03:34.07 Positioning Slug by 0.324ml @22:03:34.16 AS.seek 0.326ml @22:09:00.26 delay 5 seconds @22:09:05.29 ATV.dial ARV @22:09:06.07 ASV.dial :PCRlock @22:09:06.49 delay 5 seconds @22:09:11.51 Running CG Profile60C300 thermocycling @22:09:11.52 PCR.program Profile60C300 @22:09:11.65 Profile60C300 should complete in < 1:49:17 @22:09:11.67 PCR.run 2:21:36.25 @23:31:23.59 PCR.off @23:31:25.62 PCR for CG completed -- closed /var/log/esp/CG20mar1621h.pcr @23:31:25.70 Cleaning PCR @23:31:25.71 AFV.select :carrierSolution @23:31:25.84 delay 2 seconds @23:31:27.86 AS.reconfigure ASconfig @23:31:27.92 AS.seek 0.5ml @23:31:38.22 delay 1 second @23:31:39.25 AFV.select :holdingCoil @23:31:39.35 ASV.dial :waste1 @23:31:39.74 ATV.dial :air3 @23:31:41.03 AS.reconfigure ASslow1 @23:31:41.09 AS.seek 0.51ml @23:31:43.42 delay 2 seconds @23:31:45.44 ATV.dial :bleach @23:31:46.07 AS.seek 0.81ml @23:32:46.67 delay 3 seconds @23:32:49.70 ATV.dial :air3 @23:32:50.27 delay 3 seconds @23:32:53.34 AS.seek 0.835ml @23:32:58.67 delay 3 seconds @23:33:01.69 ATV.dial :bleach @23:33:02.33 AS.seek 0.82ml @23:33:05.56 delay 3 seconds @23:33:08.59 ARV.dial :waste @23:33:09.21 delay 3 seconds @23:33:12.23 ATV.dial :ARV @23:33:13.12 AS.reconfigure ASconfig @23:33:13.18 AS.seek 0.47ml @23:33:33.61 AFV.select :carrierSolution @23:33:33.73 delay 2 seconds @23:33:35.75 AS.seek 1ml @23:34:06.50 delay 1 second @23:34:07.53 AFV.select :holdingCoil @23:34:07.63 delay 1 second @23:34:08.65 AS.reconfigure ASfast @23:34:08.71 AS.seek 0ml @23:34:36.58 AFV.select :carrierSolution @23:34:36.70 delay 2 seconds @23:34:38.72 AS.reconfigure ASconfig @23:34:38.78 AS.seek 1ml @23:35:36.58 delay 1 second @23:35:37.61 AFV.select :holdingCoil @23:35:37.71 delay 1 second @23:35:39.10 AS.reconfigure ASfast @23:35:39.16 AS.seek 0ml @23:36:06.87 AFV.select :carrierSolution @23:36:06.99 delay 2 seconds @23:36:09.01 AS.reconfigure ASconfig @23:36:09.07 AS.seek 1ml @23:37:06.81 delay 1 second @23:37:07.84 AFV.select :holdingCoil @23:37:07.94 delay 1 second @23:37:08.96 AS.reconfigure ASfast @23:37:09.02 AS.seek 0ml @23:37:36.87 ARV.dial between carrier and air1 @23:37:37.38 ATV.dial :air3 @23:37:38.68 AS.reconfigure ASslow1 @23:37:38.75 AS.seek 0.01ml @23:37:41.06 delay 2 seconds @23:37:43.09 ATV.dial :bleach @23:37:43.72 AS.seek 0.81ml @23:40:24.84 delay 3 seconds @23:40:27.87 ATV.dial :air3 @23:40:28.43 delay 3 seconds @23:40:31.50 AS.seek 0.835ml @23:40:36.82 delay 3 seconds @23:40:39.85 ATV.dial :bleach @23:40:40.48 AS.seek 0.82ml @23:40:43.78 delay 3 seconds @23:40:46.81 ATV.dial PCR @23:40:47.21 AS.reconfigure ASconfig @23:40:47.26 AS.seek :empty @23:41:34.73 PCR.program :bleachProfile @23:41:34.79 PCR program should complete in < 2:45 @23:41:34.80 PCR.run 10:00 @23:44:33.62 PCR.off @23:44:33.65 AFV.select :carrierSolution @23:44:33.77 delay 2 seconds @23:44:35.79 AS.seek 1ml @23:45:33.56 delay 1 second @23:45:34.59 AFV.select :holdingCoil @23:45:34.69 delay 1 second @23:45:35.71 AS.reconfigure ASfast @23:45:35.77 AS.seek 0ml @23:46:03.54 delay 3 seconds @23:46:06.56 AFV.select :carrierSolution @23:46:06.66 delay 2 seconds @23:46:08.68 AS.reconfigure ASconfig @23:46:08.78 AS.seek 1ml @23:47:06.60 delay 1 second @23:47:07.62 AFV.select :holdingCoil @23:47:07.72 delay 1 second @23:47:08.74 AS.reconfigure ASfast @23:47:08.80 AS.seek 0ml @23:47:36.43 delay 3 seconds @23:47:39.45 AFV.select :carrierSolution @23:47:39.55 delay 2 seconds @23:47:41.57 AS.reconfigure ASconfig @23:47:41.64 AS.seek 1ml @23:48:39.41 delay 1 second @23:48:40.44 AFV.select :holdingCoil @23:48:40.54 delay 1 second @23:48:41.56 AS.reconfigure ASfast @23:48:41.62 AS.seek 0ml @23:49:09.57 delay 3 seconds @23:49:12.59 AFV.select :carrierSolution @23:49:12.69 delay 2 seconds @23:49:14.71 AS.reconfigure ASconfig @23:49:14.78 AS.seek 1ml @23:50:12.59 delay 1 second @23:50:13.62 AFV.select :holdingCoil @23:50:13.72 delay 1 second @23:50:14.74 AS.reconfigure ASfast @23:50:14.80 AS.seek 0ml @23:50:42.30 delay 3 seconds @23:50:45.33 AFV.select :carrierSolution @23:50:45.43 delay 2 seconds @23:50:47.45 AS.reconfigure ASconfig @23:50:47.52 AS.seek 1ml @23:51:45.28 delay 1 second @23:51:46.31 AFV.select :holdingCoil @23:51:46.41 delay 1 second @23:51:47.43 AS.reconfigure ASnorm @23:51:47.49 AS.seek 0ml @23:52:38.24
AFV.select :carrierSolution @23:52:38.35 delay 2 seconds @23:52:40.37 AS.reconfigure ASconfig @23:52:40.43 AS.seek 0.5ml @23:53:09.47 delay 1 second @23:53:10.50 AFV.select :holdingCoil @23:53:10.61 ATV.dial :air1 @23:53:12.11 AS.reconfigure ASslow1 @23:53:12.17 AS.seek 0.52ml @23:53:16.57 delay 5 seconds @23:53:21.59 ATV.dial :extSrc @23:53:23.29 AS.reconfigure ASslow2 @23:53:23.35 AS.seek 0.545ml @23:53:48.83 delay 10 seconds @23:53:58.86 ATV.dial :air1 @23:54:00.59 AS.reconfigure ASslow1 @23:54:00.65 AS.seek 0.575ml @23:54:07.02 ATV.dial :waste @23:54:07.59 AS.reconfigure ASconfig @23:54:07.64 AS.seek :empty @23:54:41.55 Setting up PCR for CG -- writing /var/log/esp/CG20mar1623h.pcr @23:54:41.57 Building PCR Slug from extSrc with MM1 mix, PP2 primer @23:54:41.60 Priming with carrier @23:54:41.62 ATV.dial ARV @23:54:43.13 ARV.dial :carrier @23:54:43.48 AS.reconfigure ASnorm @23:54:43.54 AS.seek 0.499ml @23:55:09.11 AFV.select :carrierSolution @23:55:09.24 delay 2 seconds @23:55:11.26 AS.reconfigure ASconfig @23:55:11.33 AS.seek 1ml @23:55:40.43 delay 1 second @23:55:41.45 AFV.select :holdingCoil @23:55:41.55 delay 1 second @23:55:42.57 ATV.dial :waste @23:55:44.26 AS.reconfigure ASnorm @23:55:44.32 AS.seek :empty @23:56:35.09 AFV.select :carrierSolution @23:56:35.22 delay 2 seconds @23:56:37.24 AS.reconfigure ASconfig @23:56:37.30 AS.seek 0.5ml @23:57:06.38 delay 1 second @23:57:07.40 AFV.select :holdingCoil @23:57:07.50 Building Slug @23:57:07.51 ATV.dial :ARV @23:57:09.18 delay 2 seconds @23:57:11.20 ARV.dial :air1 @23:57:11.60 delay 2 seconds @23:57:13.67 AS.reconfigure ASslow2 @23:57:13.73 AS.seek 0.506ml @23:57:19.93 delay 2 seconds @23:57:21.95 ARV.dial :MM1 @23:57:22.56 delay 2 seconds @23:57:24.58 Adding MM1 @23:57:24.66 AS.seek 0.524ml @23:57:43.17 delay 25 seconds @23:58:08.19 ARV.dial :PP2 @23:58:08.59 Adding PP2 @23:58:08.60 delay 2 seconds @23:58:10.68 AS.seek 0.53ml @23:58:16.92 delay 10 seconds @23:58:26.95 ARV.dial :air2 @23:58:27.34 adding ARV air @23:58:27.35 delay 2 seconds @23:58:29.43 AS.seek 0.536ml @23:58:35.84 delay 5 seconds @23:58:40.87 ARV.dial :carrier @23:58:42.17 delay 7 seconds @23:58:49.25 AS.seek 0.594ml @23:59:48.16 delay 5 seconds @23:59:53.19 ATV.dial :extSrc @23:59:53.76 delay 2 seconds @23:59:55.83 AS.seek 0.6ml @00:00:02.23CET17-Mar-20 delay 4 seconds @00:00:06.25 ATV.dial ARV @00:00:06.82 delay 2 seconds @00:00:08.84 ARV.dial :carrier @00:00:08.85 delay 2 seconds @00:00:10.93 AS.seek 0.65ml @00:01:01.68 delay 4 seconds @00:01:05.70 ATV.dial PCR @00:01:06.47 ASV.dial :waste1 @00:01:06.49 ARV.dial :air1 @00:01:06.93 Positioning Slug by 0.324ml @00:01:07.02 AS.seek 0.326ml @00:06:33.31 delay 5 seconds @00:06:38.34 ATV.dial ARV @00:06:39.13 ASV.dial :PCRlock @00:06:39.54 delay 5 seconds @00:06:44.56 Running CG Profile60C300 thermocycling @00:06:44.57 PCR.program Profile60C300 @00:06:44.70 Profile60C300 should complete in < 1:49:17 @00:06:44.72 PCR.run 2:21:36.25 @01:28:53.89 PCR.off @01:28:55.93 PCR for CG completed -- closed /var/log/esp/CG20mar1623h.pcr @01:28:56.01 Cleaning PCR @01:28:56.02 AFV.select :carrierSolution @01:28:56.14 delay 2 seconds @01:28:58.16 AS.reconfigure ASconfig @01:28:58.22 AS.seek 0.5ml @01:29:08.49 delay 1 second @01:29:09.52 AFV.select :holdingCoil @01:29:09.62 ASV.dial :waste1 @01:29:10.03 ATV.dial :air3 @01:29:11.33 AS.reconfigure ASslow1 @01:29:11.40 AS.seek 0.51ml @01:29:13.67 delay 2 seconds @01:29:15.70 ATV.dial :bleach @01:29:16.31 AS.seek 0.81ml @01:30:16.91 delay 3 seconds @01:30:19.94 ATV.dial :air3 @01:30:20.51 delay 3 seconds @01:30:23.58 AS.seek 0.835ml @01:30:28.89 delay 3 seconds @01:30:31.92 ATV.dial :bleach @01:30:32.55 AS.seek 0.82ml @01:30:35.90 delay 3 seconds @01:30:38.93 ARV.dial :waste @01:30:39.56 delay 3 seconds @01:30:42.58 ATV.dial :ARV @01:30:43.47 AS.reconfigure ASconfig @01:30:43.53 AS.seek 0.47ml @01:31:03.96 AFV.select :carrierSolution @01:31:04.08 delay 2 seconds @01:31:06.10 AS.seek 1ml @01:31:36.85 delay 1 second @01:31:37.88 AFV.select :holdingCoil @01:31:37.98 delay 1 second @01:31:39.00 AS.reconfigure ASfast @01:31:39.06 AS.seek 0ml @01:32:06.60 AFV.select :carrierSolution @01:32:06.72 delay 2 seconds @01:32:08.74 AS.reconfigure ASconfig @01:32:08.80 AS.seek 1ml @01:33:06.58 delay 1 second @01:33:07.61 AFV.select :holdingCoil @01:33:07.71 delay 1 second @01:33:08.73 AS.reconfigure ASfast @01:33:08.79 AS.seek 0ml @01:33:36.43 AFV.select :carrierSolution @01:33:36.55 delay 2 seconds @01:33:38.57 AS.reconfigure ASconfig @01:33:38.63 AS.seek 1ml @01:34:36.36 delay 1 second @01:34:37.39 AFV.select :holdingCoil @01:34:37.49 delay 1 second @01:34:38.51 AS.reconfigure ASfast @01:34:38.95 AS.seek 0ml @01:35:06.38 ARV.dial between carrier and air1 @01:35:06.89 ATV.dial :air3 @01:35:08.21 AS.reconfigure ASslow1 @01:35:08.28 AS.seek 0.01ml @01:35:10.58 delay 2 seconds @01:35:12.60 ATV.dial :bleach @01:35:13.23 AS.seek 0.81ml @01:37:54.35 delay 3 seconds @01:37:57.38 ATV.dial :air3 @01:37:57.95 delay 3 seconds @01:38:01.02 AS.seek 0.835ml @01:38:06.33 delay 3 seconds @01:38:09.36 ATV.dial :bleach @01:38:10.00 AS.seek 0.82ml @01:38:13.30 delay 3 seconds @01:38:16.32 ATV.dial PCR @01:38:16.72 AS.reconfigure ASconfig @01:38:16.77 AS.seek :empty @01:39:04.24 PCR.program :bleachProfile @01:39:04.30 PCR program should complete in < 2:45 @01:39:04.31 PCR.run 10:00 @01:42:03.11 PCR.off @01:42:03.14 AFV.select :carrierSolution @01:42:03.26 delay 2 seconds @01:42:05.28 AS.seek 1ml @01:43:03.11 delay 1 second @01:43:04.14 AFV.select :holdingCoil @01:43:04.24 delay 1 second @01:43:05.26 AS.reconfigure ASfast @01:43:05.32 AS.seek 0ml @01:43:32.92 delay 3 seconds @01:43:35.94 AFV.select :carrierSolution @01:43:36.04 delay 2 seconds @01:43:38.06 AS.reconfigure ASconfig @01:43:38.12 AS.seek 1ml @01:44:35.87 delay 1 second @01:44:36.89 AFV.select :holdingCoil @01:44:36.99 delay 1 second @01:44:38.01 AS.reconfigure ASfast @01:44:38.07 AS.seek 0ml @01:45:05.77 delay 3 seconds @01:45:08.79 AFV.select :carrierSolution @01:45:08.89 delay 2 seconds @01:45:10.91 AS.reconfigure ASconfig @01:45:10.98 AS.seek 1ml @01:46:08.76 delay 1 second @01:46:09.79 AFV.select :holdingCoil @01:46:09.89 delay 1 second @01:46:10.91 AS.reconfigure ASfast @01:46:10.97 AS.seek 0ml @01:46:38.53 delay 3 seconds @01:46:41.55 AFV.select :carrierSolution @01:46:41.65 delay 2 seconds @01:46:43.67 AS.reconfigure ASconfig @01:46:43.74 AS.seek 1ml @01:47:41.47 delay 1 second @01:47:42.50 AFV.select :holdingCoil @01:47:42.60 delay 1 second @01:47:43.62 AS.reconfigure ASfast @01:47:43.68 AS.seek 0ml @01:48:11.03 delay 3 seconds @01:48:14.05 AFV.select :carrierSolution @01:48:14.15 delay 2 seconds @01:48:16.17 AS.reconfigure ASconfig @01:48:16.23 AS.seek 1ml @01:49:13.97 delay 1 second @01:49:15.00 AFV.select :holdingCoil @01:49:15.10 delay 1 second @01:49:16.12 AS.reconfigure ASnorm @01:49:16.18 AS.seek 0ml @01:50:06.95
AFV.select :carrierSolution @01:50:07.06 delay 2 seconds @01:50:09.08 AS.reconfigure ASconfig @01:50:09.14 AS.seek 0.5ml @01:50:38.18 delay 1 second @01:50:39.21 AFV.select :holdingCoil @01:50:39.31 ATV.dial :air1 @01:50:40.81 AS.reconfigure ASslow2 @01:50:40.87 AS.seek 0.506ml @01:50:47.08 ATV.dial :extSrc @01:50:48.80 AS.seek 0.512ml @01:50:55.22 delay 5 seconds @01:51:00.25 ATV.dial :air1 @01:51:01.97 AS.reconfigure ASslow1 @01:51:02.03 AS.seek 0.542ml @01:51:08.37 ATV.dial :waste @01:51:08.94 AS.reconfigure ASconfig @01:51:08.99 AS.seek :empty @01:51:40.67 Setting up PCR for CG -- writing /var/log/esp/CG20mar1701h.pcr @01:51:40.70 Building PCR Slug from extSrc with MM1 mix, PP2 primer @01:51:40.72 Priming with carrier @01:51:40.74 ATV.dial ARV @01:51:42.25 ARV.dial :carrier @01:51:42.58 AS.reconfigure ASnorm @01:51:42.65 AS.seek 0.499ml @01:52:08.60 AFV.select :carrierSolution @01:52:08.72 delay 2 seconds @01:52:10.74 AS.reconfigure ASconfig @01:52:10.81 AS.seek 1ml @01:52:39.89 delay 1 second @01:52:40.91 AFV.select :holdingCoil @01:52:41.01 delay 1 second @01:52:42.03 ATV.dial :waste @01:52:43.72 AS.reconfigure ASnorm @01:52:43.79 AS.seek :empty @01:53:34.52 AFV.select :carrierSolution @01:53:34.65 delay 2 seconds @01:53:36.67 AS.reconfigure ASconfig @01:53:36.73 AS.seek 0.5ml @01:54:05.86 delay 1 second @01:54:06.88 AFV.select :holdingCoil @01:54:06.98 Building Slug @01:54:06.99 ATV.dial :ARV @01:54:08.66 delay 2 seconds @01:54:10.68 ARV.dial :air1 @01:54:11.08 delay 2 seconds @01:54:13.15 AS.reconfigure ASslow2 @01:54:13.21 AS.seek 0.506ml @01:54:19.66 delay 2 seconds @01:54:21.69 ARV.dial :MM1 @01:54:22.30 delay 2 seconds @01:54:24.32 Adding MM1 @01:54:24.40 AS.seek 0.524ml @01:54:42.92 delay 25 seconds @01:55:07.94 ARV.dial :PP2 @01:55:08.33 Adding PP2 @01:55:08.34 delay 2 seconds @01:55:10.42 AS.seek 0.53ml @01:55:16.88 delay 10 seconds @01:55:26.91 ARV.dial :air2 @01:55:27.31 adding ARV air @01:55:27.32 delay 2 seconds @01:55:29.40 AS.seek 0.536ml @01:55:35.94 delay 5 seconds @01:55:40.96 ARV.dial :carrier @01:55:42.25 delay 7 seconds @01:55:49.33 AS.seek 0.594ml @01:56:47.89 delay 5 seconds @01:56:52.91 ATV.dial :extSrc @01:56:53.48 delay 2 seconds @01:56:55.55 AS.seek 0.6ml @01:57:01.74 delay 4 seconds @01:57:05.77 ATV.dial ARV @01:57:06.33 delay 2 seconds @01:57:08.35 ARV.dial :carrier @01:57:08.36 delay 2 seconds @01:57:10.44 AS.seek 0.65ml @01:58:00.89 delay 4 seconds @01:58:04.91 ATV.dial PCR @01:58:05.68 ASV.dial :waste1 @01:58:05.70 ARV.dial :air1 @01:58:06.13 Positioning Slug by 0.324ml @01:58:06.22 AS.seek 0.326ml @02:03:32.31 delay 5 seconds @02:03:37.34 ATV.dial ARV @02:03:38.16 ASV.dial :PCRlock @02:03:38.58 delay 5 seconds @02:03:43.60 Running CG Profile60C300 thermocycling @02:03:43.62 PCR.program Profile60C300 @02:03:43.82 Profile60C300 should complete in < 1:49:17 @02:03:43.84 PCR.run 2:21:36.25 @02:53:11.38 Session Ended @03:25:58.73 PCR.off @03:26:00.76 PCR for CG completed -- closed /var/log/esp/CG20mar1701h.pcr @03:26:00.82 Cleaning PCR @03:26:00.83 AFV.select :carrierSolution @03:26:00.93 delay 2 seconds @03:26:02.95 AS.reconfigure ASconfig @03:26:03.00 AS.seek 0.5ml @03:26:13.29 delay 1 second @03:26:14.32 AFV.select :holdingCoil @03:26:14.42 ASV.dial :waste1 @03:26:14.81 ATV.dial :air3 @03:26:16.10 AS.reconfigure ASslow1 @03:26:16.15 AS.seek 0.51ml @03:26:18.41 delay 2 seconds @03:26:20.44 ATV.dial :bleach @03:26:21.06 AS.seek 0.81ml @03:27:21.67 delay 3 seconds @03:27:24.70 ATV.dial :air3 @03:27:25.26 delay 3 seconds @03:27:28.33 AS.seek 0.835ml @03:27:33.69 delay 3 seconds @03:27:36.72 ATV.dial :bleach @03:27:37.34 AS.seek 0.82ml @03:27:40.64 delay 3 seconds @03:27:43.67 ARV.dial :waste @03:27:44.27 delay 3 seconds @03:27:47.29 ATV.dial :ARV @03:27:48.16 AS.reconfigure ASconfig @03:27:48.20 AS.seek 0.47ml @03:28:08.60 AFV.select :carrierSolution @03:28:08.71 delay 2 seconds @03:28:10.73 AS.seek 1ml @03:28:41.47 delay 1 second @03:28:42.49 AFV.select :holdingCoil @03:28:42.59 delay 1 second @03:28:43.61 AS.reconfigure ASfast @03:28:43.66 AS.seek 0ml @03:29:11.31 AFV.select :carrierSolution @03:29:11.42 delay 2 seconds @03:29:13.44 AS.reconfigure ASconfig @03:29:13.49 AS.seek 1ml @03:30:11.21 delay 1 second @03:30:12.24 AFV.select :holdingCoil @03:30:12.34 delay 1 second @03:30:13.36 AS.reconfigure ASfast @03:30:13.41 AS.seek 0ml @03:30:41.18 AFV.select :carrierSolution @03:30:41.29 delay 2 seconds @03:30:43.31 AS.reconfigure ASconfig @03:30:43.36 AS.seek 1ml @03:31:41.10 delay 1 second @03:31:42.13 AFV.select :holdingCoil @03:31:42.23 delay 1 second @03:31:43.25 AS.reconfigure ASfast @03:31:43.30 AS.seek 0ml @03:32:10.81 ARV.dial between carrier and air1 @03:32:11.30 ATV.dial :air3 @03:32:12.59 AS.reconfigure ASslow1 @03:32:12.64 AS.seek 0.01ml @03:32:14.92 delay 2 seconds @03:32:16.95 ATV.dial :bleach @03:32:17.94 AS.seek 0.81ml @03:34:59.07 delay 3 seconds @03:35:02.10 ATV.dial :air3 @03:35:02.66 delay 3 seconds @03:35:05.73 AS.seek 0.835ml @03:35:11.01 delay 3 seconds @03:35:14.04 ATV.dial :bleach @03:35:14.66 AS.seek 0.82ml @03:35:17.95 delay 3 seconds @03:35:20.98 ATV.dial PCR @03:35:21.37 AS.reconfigure ASconfig @03:35:21.41 AS.seek :empty @03:36:08.84 PCR.program :bleachProfile @03:36:08.90 PCR program should complete in < 2:45 @03:36:08.91 PCR.run 10:00 @03:39:07.71 PCR.off @03:39:07.74 AFV.select :carrierSolution @03:39:07.84 delay 2 seconds @03:39:09.86 AS.seek 1ml @03:40:07.65 delay 1 second @03:40:08.68 AFV.select :holdingCoil @03:40:08.78 delay 1 second @03:40:09.80 AS.reconfigure ASfast @03:40:09.85 AS.seek 0ml @03:40:37.46 delay 3 seconds @03:40:40.48 AFV.select :carrierSolution @03:40:40.58 delay 2 seconds @03:40:42.60 AS.reconfigure ASconfig @03:40:42.65 AS.seek 1ml @03:41:40.41 delay 1 second @03:41:41.43 AFV.select :holdingCoil @03:41:41.53 delay 1 second @03:41:42.55 AS.reconfigure ASfast @03:41:42.60 AS.seek 0ml @03:42:10.37 delay 3 seconds @03:42:13.39 AFV.select :carrierSolution @03:42:13.49 delay 2 seconds @03:42:15.51 AS.reconfigure ASconfig @03:42:15.57 AS.seek 1ml @03:43:13.36 delay 1 second @03:43:14.39 AFV.select :holdingCoil @03:43:14.49 delay 1 second @03:43:15.51 AS.reconfigure ASfast @03:43:15.56 AS.seek 0ml @03:43:43.02 delay 3 seconds @03:43:46.04 AFV.select :carrierSolution @03:43:46.14 delay 2 seconds @03:43:48.16 AS.reconfigure ASconfig @03:43:48.22 AS.seek 1ml @03:44:45.95 delay 1 second @03:44:46.98 AFV.select :holdingCoil @03:44:47.08 delay 1 second @03:44:48.10 AS.reconfigure ASfast @03:44:48.15 AS.seek 0ml @03:45:15.61 delay 3 seconds @03:45:18.64 AFV.select :carrierSolution @03:45:18.74 delay 2 seconds @03:45:20.76 AS.reconfigure ASconfig @03:45:20.82 AS.seek 1ml @03:46:18.54 delay 1 second @03:46:19.57 AFV.select :holdingCoil @03:46:19.67 delay 1 second @03:46:20.69 AS.reconfigure ASnorm @03:46:20.74 AS.seek 0ml @03:47:11.47
AFV.select :carrierSolution @03:47:11.57 delay 2 seconds @03:47:13.59 AS.reconfigure ASconfig @03:47:13.64 AS.seek 0.5ml @03:47:42.67 delay 1 second @03:47:43.70 AFV.select :holdingCoil @03:47:43.79 ATV.dial :air1 @03:47:45.28 AS.reconfigure ASslow1 @03:47:45.32 AS.seek 0.52ml @03:47:49.64 delay 5 seconds @03:47:54.67 ATV.dial :extSrc @03:47:56.37 AS.reconfigure ASslow2 @03:47:56.41 AS.seek 0.538ml @03:48:14.64 delay 10 seconds @03:48:24.67 ATV.dial :air1 @03:48:26.37 AS.reconfigure ASslow1 @03:48:26.41 AS.seek 0.568ml @03:48:32.75 ATV.dial :waste @03:48:33.30 AS.reconfigure ASconfig @03:48:33.34 AS.seek :empty @03:49:06.32 deconEluateRecoveryLine extSrc @03:49:06.33 ATV.dial :waste @03:49:06.35 AS.reconfigure ASnorm @03:49:06.39 AS.seek 0ml @03:49:06.79 ATV.dial :air3 @03:49:07.46 AS.reconfigure ASslow1 @03:49:07.51 AS.seek 0.01ml @03:49:09.76 delay 2 seconds @03:49:11.79 ATV.dial :bleach @03:49:12.24 AS.seek 0.31ml @03:50:12.95 delay 3 seconds @03:50:15.98 ATV.dial :air3 @03:50:16.54 delay 3 seconds @03:50:19.61 AS.seek 0.335ml @03:50:24.93 delay 3 seconds @03:50:27.96 ATV.dial :bleach @03:50:28.57 AS.seek 0.32ml @03:50:31.87 delay 3 seconds @03:50:35.25 ATV.dial :extSrc @03:50:35.90 AS.seek 0ml @03:51:40.51 AFV.select :carrierSolution @03:51:40.62 delay 2 seconds @03:51:42.64 AS.reconfigure ASconfig @03:51:42.69 AS.seek 0.5ml @03:52:11.71 delay 1 second @03:52:12.73 AFV.select :holdingCoil @03:52:12.83 ATV.dial :extSrc @03:52:12.84 AS.reconfigure ASslow1 @03:52:12.89 AS.seek 0ml @03:53:53.70 AFV.select :carrierSolution @03:53:53.81 delay 2 seconds @03:53:55.83 AS.reconfigure ASconfig @03:53:55.88 AS.seek 0.5ml @03:54:24.91 delay 1 second @03:54:25.93 AFV.select :holdingCoil @03:54:26.03 ATV.dial :extSrc @03:54:26.05 AS.reconfigure ASslow1 @03:54:26.09 AS.seek 0ml @03:56:06.89 AFV.select :carrierSolution @03:56:07.00 delay 2 seconds @03:56:09.02 AS.reconfigure ASconfig @03:56:09.07 AS.seek 0.5ml @03:56:38.09 delay 1 second @03:56:39.12 AFV.select :holdingCoil @03:56:39.22 ATV.dial :extSrc @03:56:39.23 AS.reconfigure ASslow1 @03:56:39.28 AS.seek 0ml @03:58:20.11 AFV.select :carrierSolution @03:58:20.22 delay 2 seconds @03:58:22.24 AS.reconfigure ASconfig @03:58:22.29 AS.seek 0.5ml @03:58:51.32 delay 1 second @03:58:52.34 AFV.select :holdingCoil @03:58:52.44 ATV.dial :extSrc @03:58:52.45 AS.reconfigure ASslow1 @03:58:52.50 AS.seek 0ml @04:00:33.31 ATV.dial :air2 @04:00:34.64 AS.reconfigure ASslow0 @04:00:34.68 AS.seek 0.5ml @04:01:25.33 ATV.dial :extSrc @04:01:26.81 AS.seek 0.02ml @04:02:15.41 delay 1 second @04:02:16.43 ATV.dial :waste @04:02:17.71 AS.reconfigure ASconfig @04:02:17.75 AS.seek :empty @04:02:19.25 deconEluateRecoveryLine extSrc @04:02:19.26 ATV.dial :waste @04:02:19.28 AS.reconfigure ASnorm @04:02:19.32 AS.seek 0ml @04:02:19.65 ATV.dial :air3 @04:02:20.49 AS.reconfigure ASslow1 @04:02:20.54 AS.seek 0.01ml @04:02:22.81 delay 2 seconds @04:02:24.84 ATV.dial :bleach @04:02:25.28 AS.seek 0.31ml @04:03:25.87 delay 3 seconds @04:03:28.90 ATV.dial :air3 @04:03:29.46 delay 3 seconds @04:03:32.53 AS.seek 0.335ml @04:03:37.84 delay 3 seconds @04:03:40.87 ATV.dial :bleach @04:03:41.48 AS.seek 0.32ml @04:03:44.79 delay 3 seconds @04:03:47.82 ATV.dial :extSrc @04:03:48.43 AS.seek 0ml @04:04:53.03 AFV.select :carrierSolution @04:04:53.14 delay 2 seconds @04:04:55.16 AS.reconfigure ASconfig @04:04:55.21 AS.seek 0.5ml @04:05:24.24 delay 1 second @04:05:25.26 AFV.select :holdingCoil @04:05:25.36 ATV.dial :extSrc @04:05:25.37 AS.reconfigure ASslow1 @04:05:25.42 AS.seek 0ml @04:07:06.23 AFV.select :carrierSolution @04:07:06.34 delay 2 seconds @04:07:08.36 AS.reconfigure ASconfig @04:07:08.41 AS.seek 0.5ml @04:07:37.43 delay 1 second @04:07:38.46 AFV.select :holdingCoil @04:07:38.56 ATV.dial :extSrc @04:07:38.57 AS.reconfigure ASslow1 @04:07:38.62 AS.seek 0ml @04:09:19.42 AFV.select :carrierSolution @04:09:19.53 delay 2 seconds @04:09:21.55 AS.reconfigure ASconfig @04:09:21.60 AS.seek 0.5ml @04:09:50.62 delay 1 second @04:09:51.65 AFV.select :holdingCoil @04:09:51.75 ATV.dial :extSrc @04:09:51.76 AS.reconfigure ASslow1 @04:09:51.81 AS.seek 0ml @04:11:32.64 AFV.select :carrierSolution @04:11:32.75 delay 2 seconds @04:11:34.77 AS.reconfigure ASconfig @04:11:34.82 AS.seek 0.5ml @04:12:03.84 delay 1 second @04:12:04.87 AFV.select :holdingCoil @04:12:04.97 ATV.dial :extSrc @04:12:04.98 AS.reconfigure ASslow1 @04:12:05.03 AS.seek 0ml @04:13:45.84 ATV.dial :air2 @04:13:47.17 AS.reconfigure ASslow0 @04:13:47.21 AS.seek 0.5ml @04:14:37.83 ATV.dial :extSrc @04:14:39.31 AS.seek 0.02ml @04:15:27.89 delay 1 second @04:15:28.92 ATV.dial :waste @04:15:30.20 AS.reconfigure ASconfig @04:15:30.24 AS.seek :empty @04:15:31.73 deconEluateRecoveryLine extSrc @04:15:31.74 ATV.dial :waste @04:15:31.76 AS.reconfigure ASnorm @04:15:31.80 AS.seek 0ml @04:15:32.20 ATV.dial :air3 @04:15:33.39 AS.reconfigure ASslow1 @04:15:33.44 AS.seek 0.01ml @04:15:35.71 delay 2 seconds @04:15:37.73 ATV.dial :bleach @04:15:38.18 AS.seek 0.31ml @04:16:38.76 delay 3 seconds @04:16:41.78 ATV.dial :air3 @04:16:42.34 delay 3 seconds @04:16:45.41 AS.seek 0.335ml @04:16:50.70 delay 3 seconds @04:16:53.73 ATV.dial :bleach @04:16:54.35 AS.seek 0.32ml @04:16:57.62 delay 3 seconds @04:17:00.65 ATV.dial :extSrc @04:17:01.26 AS.seek 0ml @04:18:05.87 AFV.select :carrierSolution @04:18:05.98 delay 2 seconds @04:18:08.00 AS.reconfigure ASconfig @04:18:08.05 AS.seek 0.5ml @04:18:37.09 delay 1 second @04:18:38.11 AFV.select :holdingCoil @04:18:38.21 ATV.dial :extSrc @04:18:38.22 AS.reconfigure ASslow1 @04:18:38.27 AS.seek 0ml @04:20:19.08 AFV.select :carrierSolution @04:20:19.19 delay 2 seconds @04:20:21.21 AS.reconfigure ASconfig @04:20:21.26 AS.seek 0.5ml @04:20:50.28 delay 1 second @04:20:51.31 AFV.select :holdingCoil @04:20:51.41 ATV.dial :extSrc @04:20:51.42 AS.reconfigure ASslow1 @04:20:51.47 AS.seek 0ml @04:22:32.30 AFV.select :carrierSolution @04:22:32.41 delay 2 seconds @04:22:34.43 AS.reconfigure ASconfig @04:22:34.48 AS.seek 0.5ml @04:23:03.51 delay 1 second @04:23:04.53 AFV.select :holdingCoil @04:23:04.63 ATV.dial :extSrc @04:23:04.64 AS.reconfigure ASslow1 @04:23:04.69 AS.seek 0ml @04:24:45.53 AFV.select :carrierSolution @04:24:45.64 delay 2 seconds @04:24:47.66 AS.reconfigure ASconfig @04:24:47.71 AS.seek 0.5ml @04:25:16.74 delay 1 second @04:25:17.76 AFV.select :holdingCoil @04:25:17.86 ATV.dial :extSrc @04:25:17.87 AS.reconfigure ASslow1 @04:25:17.92 AS.seek 0ml @04:26:58.75 ATV.dial :air2 @04:27:00.07 AS.reconfigure ASslow0 @04:27:00.11 AS.seek 0.5ml @04:27:50.72 ATV.dial :extSrc @04:27:52.20 AS.seek 0.02ml @04:28:40.81 delay 1 second @04:28:41.83 ATV.dial :waste @04:28:43.12 AS.reconfigure ASconfig @04:28:43.16 AS.seek :empty @16:26:10.68CET17-Mar-20
ESP2 v3.30 I2C Gateway v3.06 for core bus @16:26:13.07 Copyright 2018 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @16:26:13.42 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @16:26:18.59 Polling Can every 10:00 @16:26:20.69 SPE.coast @16:26:20.74 SPE.configure SPEconfig @16:26:20.84 AS.reconfigure ASconfig @16:26:20.87 AS.hold @16:26:20.92 AS.configure ASconfig @16:26:21.00 SS.reconfigure SSconfig @16:26:21.04 SS.hold @16:26:21.09 SS.configure SSconfig @16:26:21.17 CC.reconfigure CCconfig @16:26:21.21 CC.hold @16:26:21.31 CC.configure CCconfig @16:26:21.47 CS.reconfigure CSconfig @16:26:21.55 CS.hold @16:26:21.65 CS.configure CSconfig @16:26:22.27 PC.reconfigure PCconfig @16:26:22.31 PC.hold @16:26:22.38 PC.configure PCconfig @16:26:22.46 PS.configure PSconfig @16:26:22.56 Forearm.reconfigure LooseForearm @16:26:22.60 Forearm.hold @16:26:22.65 Forearm.configure LooseForearm @16:26:22.73 Elbow.reconfigure LooseElbow @16:26:22.77 Elbow.hold @16:26:22.86 Elbow.configure LooseElbow @16:26:23.06 SC.reconfigure SCloose @16:26:23.14 SC.hold @16:26:23.24 SC.configure SCloose @16:26:23.40 SE.reconfigure SEconfig @16:26:23.46 SE.hold @16:26:23.56 SE.configure SEconfig @16:26:23.63 CH.coast @16:26:23.67 CH.configure CHconfig @16:26:23.70 PH.coast @16:26:23.75 PH.configure PHconfig @16:26:23.78 PCR.off @16:26:23.81 PCR.pidtemp= 50,0.1,0,300,25,25 @16:26:23.83 PCR.caltemp= 2.8814,0.0372,0 @16:26:23.86 All dwarf controllers running firmware version 3.75 @16:26:24.02 Cleanly exited at 16:25:16CET17-Mar-20 @16:26:24.05 Loading /home/esp/esp2/mission/stdMER2017.rb mission @16:26:24.86 AFV.select :carrierSolution @16:26:24.98 delay 2 seconds @16:26:25.13 Session Begun @16:26:27.02
AS.seek 0.5ml @16:26:56.13 delay 1 second @16:26:57.15 AFV.select :holdingCoil @16:26:57.26 ATV.dial :extSrc @16:26:58.74 AS.reconfigure ASslow1 @16:26:58.80 AS.seek 0.53ml @16:27:05.16 delay 5 seconds @16:27:10.24 AS.reconfigure ASslow2 @16:27:10.30 AS.seek 0.54ml @16:27:20.72 delay 20 seconds @16:27:40.79 AS.reconfigure ASslow1 @16:27:40.85 AS.seek 0.57ml @16:27:47.15 delay 5 seconds @16:27:52.23 AS.reconfigure ASslow2 @16:27:52.29 AS.seek 0.58ml @16:28:02.54 delay 20 seconds @16:28:22.61 AS.reconfigure ASslow1 @16:28:22.67 AS.seek 0.61ml @16:28:28.94 delay 5 seconds @16:28:34.02 AS.reconfigure ASslow2 @16:28:34.08 AS.seek 0.62ml @16:28:44.47 delay 20 seconds @16:29:04.55 AS.reconfigure ASslow1 @16:29:04.62 AS.seek 0.65ml @16:29:10.96 delay 5 seconds @16:29:16.04 AS.reconfigure ASslow2 @16:29:16.10 AS.seek 0.66ml @16:29:26.54 delay 20 seconds @16:29:46.57 AS.reconfigure ASslow1 @16:29:46.62 AS.seek 0.705ml @16:29:55.96 delay 10 seconds @16:30:05.98 ATV.dial :air1 @16:30:07.69 AS.seek 0.725ml @16:30:11.99 delay 3 seconds @16:30:15.01 ATV.dial :waste @16:30:15.57 AS.reconfigure ASslow0 @16:30:15.63 AS.seek :empty @16:31:29.05 Setting up PCR for NM -- writing /var/log/esp/NM20mar1716h.pcr @16:31:29.07 Building PCR Slug from extSrc with MM1 mix, PP1 primer @16:31:29.10 Priming with carrier @16:31:29.12 ATV.dial ARV @16:31:30.61 ARV.dial :carrier @16:31:30.96 AS.reconfigure ASnorm @16:31:31.02 AS.seek 0.499ml @16:31:56.58 AFV.select :carrierSolution @16:31:56.69 delay 2 seconds @16:31:58.72 AS.reconfigure ASconfig @16:31:58.78 AS.seek 1ml @16:32:27.85 delay 1 second @16:32:28.87 AFV.select :holdingCoil @16:32:28.97 delay 1 second @16:32:29.99 ATV.dial :waste @16:32:31.66 AS.reconfigure ASnorm @16:32:31.72 AS.seek :empty @16:33:22.49 AFV.select :carrierSolution @16:33:22.60 delay 2 seconds @16:33:24.63 AS.reconfigure ASconfig @16:33:24.68 AS.seek 0.5ml @16:33:53.80 delay 1 second @16:33:54.82 AFV.select :holdingCoil @16:33:54.93 Building Slug @16:33:54.94 ATV.dial :ARV @16:33:56.58 delay 2 seconds @16:33:58.60 ARV.dial :air1 @16:33:59.00 delay 2 seconds @16:34:01.07 AS.reconfigure ASslow2 @16:34:01.13 AS.seek 0.506ml @16:34:07.35 delay 2 seconds @16:34:09.37 ARV.dial :MM1 @16:34:09.99 delay 2 seconds @16:34:12.01 Adding MM1 @16:34:12.10 AS.seek 0.524ml @16:34:30.58 delay 25 seconds @16:34:55.60 ARV.dial :PP1 @16:34:56.01 Adding PP1 @16:34:56.02 delay 2 seconds @16:34:58.10 AS.seek 0.53ml @16:35:04.35 delay 10 seconds @16:35:14.38 ARV.dial :air1 @16:35:14.77 adding ARV air @16:35:14.78 delay 2 seconds @16:35:16.86 AS.seek 0.536ml @16:35:23.18 delay 5 seconds @16:35:28.20 ARV.dial :carrier @16:35:28.60 delay 7 seconds @16:35:35.69 AS.seek 0.594ml @16:36:34.25 delay 5 seconds @16:36:39.27 ATV.dial :extSrc @16:36:39.85 delay 2 seconds @16:36:41.92 AS.seek 0.6ml @16:36:48.21 delay 4 seconds @16:36:52.23 ATV.dial ARV @16:36:52.79 delay 2 seconds @16:36:54.81 ARV.dial :carrier @16:36:54.82 delay 2 seconds @16:36:56.90 AS.seek 0.65ml @16:37:47.54 delay 4 seconds @16:37:51.56 ATV.dial PCR @16:37:52.33 ASV.dial :waste1 @16:37:52.74 ARV.dial :air1 @16:37:53.33 Positioning Slug by 0.324ml @16:37:53.43 AS.seek 0.326ml @16:43:19.54 delay 5 seconds @16:43:24.56 ATV.dial ARV @16:43:25.35 ASV.dial :PCRlock @16:43:25.77 delay 5 seconds @16:43:30.80 Running NM Profile60C300 thermocycling @16:43:30.82 PCR.program Profile60C300 @16:43:30.93 Profile60C300 should complete in < 1:40:53 @16:43:30.94 PCR.run 2:11:06.25 @18:04:39.42 PCR.off @18:04:39.96 PCR for NM completed -- closed /var/log/esp/NM20mar1716h.pcr @18:04:40.04 Cleaning PCR @18:04:40.05 AFV.select :carrierSolution @18:04:40.17 delay 2 seconds @18:04:42.20 AS.reconfigure ASconfig @18:04:42.25 AS.seek 0.5ml @18:04:52.55 delay 1 second @18:04:53.57 AFV.select :holdingCoil @18:04:53.67 ASV.dial :waste1 @18:04:54.07 ATV.dial :air3 @18:04:55.36 AS.reconfigure ASslow1 @18:04:55.41 AS.seek 0.51ml @18:04:57.70 delay 2 seconds @18:04:59.72 ATV.dial :bleach @18:05:00.35 AS.seek 0.81ml @18:06:00.96 delay 3 seconds @18:06:03.98 ATV.dial :air3 @18:06:04.54 delay 3 seconds @18:06:07.61 AS.seek 0.835ml @18:06:12.91 delay 3 seconds @18:06:15.93 ATV.dial :bleach @18:06:16.55 AS.seek 0.82ml @18:06:19.84 delay 3 seconds @18:06:22.86 ARV.dial :waste @18:06:23.49 delay 3 seconds @18:06:26.51 ATV.dial :ARV @18:06:27.51 AS.reconfigure ASconfig @18:06:27.57 AS.seek 0.47ml @18:06:47.99 AFV.select :carrierSolution @18:06:48.10 delay 2 seconds @18:06:50.13 AS.seek 1ml @18:07:20.87 delay 1 second @18:07:21.89 AFV.select :holdingCoil @18:07:21.99 delay 1 second @18:07:23.02 AS.reconfigure ASfast @18:07:23.07 AS.seek 0ml @18:07:50.41 AFV.select :carrierSolution @18:07:50.52 delay 2 seconds @18:07:52.55 AS.reconfigure ASconfig @18:07:52.60 AS.seek 1ml @18:08:50.35 delay 1 second @18:08:51.37 AFV.select :holdingCoil @18:08:51.47 delay 1 second @18:08:52.50 AS.reconfigure ASfast @18:08:52.55 AS.seek 0ml @18:09:20.24 AFV.select :carrierSolution @18:09:20.35 delay 2 seconds @18:09:22.38 AS.reconfigure ASconfig @18:09:22.43 AS.seek 1ml @18:10:20.21 delay 1 second @18:10:21.23 AFV.select :holdingCoil @18:10:21.33 delay 1 second @18:10:22.36 AS.reconfigure ASfast @18:10:22.41 AS.seek 0ml @18:10:49.95 ARV.dial between carrier and air1 @18:10:50.45 ATV.dial :air3 @18:10:51.76 AS.reconfigure ASslow1 @18:10:51.82 AS.seek 0.01ml @18:10:54.13 delay 2 seconds @18:10:56.15 ATV.dial :bleach @18:10:56.79 AS.seek 0.81ml @18:13:37.93 delay 3 seconds @18:13:40.95 ATV.dial :air3 @18:13:41.51 delay 3 seconds @18:13:44.58 AS.seek 0.835ml @18:13:49.89 delay 3 seconds @18:13:52.91 ATV.dial :bleach @18:13:53.95 AS.seek 0.82ml @18:13:57.26 delay 3 seconds @18:14:00.28 ATV.dial PCR @18:14:00.68 AS.reconfigure ASconfig @18:14:00.74 AS.seek :empty @18:14:48.23 PCR.program :bleachProfile @18:14:48.29 PCR program should complete in < 2:45 @18:14:48.30 PCR.run 10:00 @18:17:44.08 PCR.off @18:17:44.11 AFV.select :carrierSolution @18:17:44.23 delay 2 seconds @18:17:46.26 AS.seek 1ml @18:18:44.05 delay 1 second @18:18:45.07 AFV.select :holdingCoil @18:18:45.17 delay 1 second @18:18:46.20 AS.reconfigure ASfast @18:18:46.25 AS.seek 0ml @18:19:13.79 delay 3 seconds @18:19:16.81 AFV.select :carrierSolution @18:19:16.91 delay 2 seconds @18:19:18.94 AS.reconfigure ASconfig @18:19:19.00 AS.seek 1ml @18:20:16.77 delay 1 second @18:20:17.79 AFV.select :holdingCoil @18:20:17.89 delay 1 second @18:20:18.92 AS.reconfigure ASfast @18:20:18.97 AS.seek 0ml @18:20:46.34 delay 3 seconds @18:20:49.36 AFV.select :carrierSolution @18:20:49.46 delay 2 seconds @18:20:51.49 AS.reconfigure ASconfig @18:20:51.54 AS.seek 1ml @18:21:49.30 delay 1 second @18:21:50.32 AFV.select :holdingCoil @18:21:50.42 delay 1 second @18:21:51.45 AS.reconfigure ASfast @18:21:51.50 AS.seek 0ml @18:22:18.98 delay 3 seconds @18:22:22.00 AFV.select :carrierSolution @18:22:22.10 delay 2 seconds @18:22:24.13 AS.reconfigure ASconfig @18:22:24.18 AS.seek 1ml @18:23:21.93 delay 1 second @18:23:22.95 AFV.select :holdingCoil @18:23:23.05 delay 1 second @18:23:24.08 AS.reconfigure ASfast @18:23:24.13 AS.seek 0ml @18:23:51.48 delay 3 seconds @18:23:54.50 AFV.select :carrierSolution @18:23:54.60 delay 2 seconds @18:23:56.63 AS.reconfigure ASconfig @18:23:56.69 AS.seek 1ml @18:24:54.45 delay 1 second @18:24:55.47 AFV.select :holdingCoil @18:24:55.57 delay 1 second @18:24:56.60 AS.reconfigure ASnorm @18:24:56.65 AS.seek 0ml @18:25:47.36
AFV.select :carrierSolution @18:25:47.47 delay 2 seconds @18:25:49.50 AS.reconfigure ASconfig @18:25:49.55 AS.seek 0.5ml @18:26:18.62 delay 1 second @18:26:19.64 AFV.select :holdingCoil @18:26:19.74 ATV.dial :air1 @18:26:21.24 AS.reconfigure ASslow2 @18:26:21.30 AS.seek 0.506ml @18:26:27.85 ATV.dial :extSrc @18:26:29.57 AS.seek 0.512ml @18:26:35.96 delay 5 seconds @18:26:40.98 ATV.dial :air1 @18:26:42.69 AS.reconfigure ASslow1 @18:26:42.75 AS.seek 0.542ml @18:26:49.10 ATV.dial :waste @18:26:49.66 AS.reconfigure ASconfig @18:26:49.72 AS.seek :empty @18:27:21.37 Setting up PCR for NM -- writing /var/log/esp/NM20mar1718h.pcr @18:27:21.39 Building PCR Slug from extSrc with MM1 mix, PP1 primer @18:27:21.42 Priming with carrier @18:27:21.44 ATV.dial ARV @18:27:22.95 ARV.dial :carrier @18:27:23.29 AS.reconfigure ASnorm @18:27:23.35 AS.seek 0.499ml @18:27:48.95 AFV.select :carrierSolution @18:27:49.06 delay 2 seconds @18:27:51.09 AS.reconfigure ASconfig @18:27:51.15 AS.seek 1ml @18:28:20.24 delay 1 second @18:28:21.26 AFV.select :holdingCoil @18:28:21.36 delay 1 second @18:28:22.38 ATV.dial :waste @18:28:24.06 AS.reconfigure ASnorm @18:28:24.13 AS.seek :empty @18:29:14.88 AFV.select :carrierSolution @18:29:14.99 delay 2 seconds @18:29:17.02 AS.reconfigure ASconfig @18:29:17.08 AS.seek 0.5ml @18:29:46.16 delay 1 second @18:29:47.18 AFV.select :holdingCoil @18:29:47.29 Building Slug @18:29:47.30 ATV.dial :ARV @18:29:48.95 delay 2 seconds @18:29:50.97 ARV.dial :air1 @18:29:51.38 delay 2 seconds @18:29:53.45 AS.reconfigure ASslow2 @18:29:53.51 AS.seek 0.506ml @18:29:59.71 delay 2 seconds @18:30:01.73 ARV.dial :MM1 @18:30:02.34 delay 2 seconds @18:30:04.36 Adding MM1 @18:30:04.45 AS.seek 0.524ml @18:30:22.74 delay 25 seconds @18:30:47.76 ARV.dial :PP1 @18:30:48.16 Adding PP1 @18:30:48.17 delay 2 seconds @18:30:50.25 AS.seek 0.53ml @18:30:56.68 delay 10 seconds @18:31:06.71 ARV.dial :air1 @18:31:07.10 adding ARV air @18:31:07.11 delay 2 seconds @18:31:09.19 AS.seek 0.536ml @18:31:15.41 delay 5 seconds @18:31:20.43 ARV.dial :carrier @18:31:20.82 delay 7 seconds @18:31:27.90 AS.seek 0.594ml @18:32:26.63 delay 5 seconds @18:32:31.65 ATV.dial :extSrc @18:32:32.23 delay 2 seconds @18:32:34.30 AS.seek 0.6ml @18:32:40.63 delay 4 seconds @18:32:44.65 ATV.dial ARV @18:32:45.21 delay 2 seconds @18:32:47.57 ARV.dial :carrier @18:32:47.66 delay 2 seconds @18:32:49.74 AS.seek 0.65ml @18:33:40.16 delay 4 seconds @18:33:44.18 ATV.dial PCR @18:33:44.96 ASV.dial :waste1 @18:33:44.97 ARV.dial :air1 @18:33:45.39 Positioning Slug by 0.324ml @18:33:45.49 AS.seek 0.326ml @18:39:11.85 delay 5 seconds @18:39:16.87 ATV.dial ARV @18:39:17.67 ASV.dial :PCRlock @18:39:18.07 delay 5 seconds @18:39:23.09 Running NM Profile60C300 thermocycling @18:39:23.11 PCR.program Profile60C300 @18:39:23.22 Profile60C300 should complete in < 1:40:53 @18:39:23.23 PCR.run 2:11:06.25 @20:00:37.69 PCR.off @20:00:38.24 PCR for NM completed -- closed /var/log/esp/NM20mar1718h.pcr @20:00:38.32 Cleaning PCR @20:00:38.33 AFV.select :carrierSolution @20:00:38.45 delay 2 seconds @20:00:40.48 AS.reconfigure ASconfig @20:00:40.53 AS.seek 0.5ml @20:00:50.82 delay 1 second @20:00:51.84 AFV.select :holdingCoil @20:00:51.94 ASV.dial :waste1 @20:00:52.34 ATV.dial :air3 @20:00:53.63 AS.reconfigure ASslow1 @20:00:53.69 AS.seek 0.51ml @20:00:56.01 delay 2 seconds @20:00:58.03 ATV.dial :bleach @20:00:58.65 AS.seek 0.81ml @20:01:59.25 delay 3 seconds @20:02:02.27 ATV.dial :air3 @20:02:02.82 delay 3 seconds @20:02:05.89 AS.seek 0.835ml @20:02:11.21 delay 3 seconds @20:02:14.23 ATV.dial :bleach @20:02:14.85 AS.seek 0.82ml @20:02:18.29 delay 3 seconds @20:02:21.31 ARV.dial :waste @20:02:21.92 delay 3 seconds @20:02:24.94 ATV.dial :ARV @20:02:25.84 AS.reconfigure ASconfig @20:02:25.90 AS.seek 0.47ml @20:02:46.32 AFV.select :carrierSolution @20:02:46.43 delay 2 seconds @20:02:48.46 AS.seek 1ml @20:03:19.25 delay 1 second @20:03:20.27 AFV.select :holdingCoil @20:03:20.37 delay 1 second @20:03:21.40 AS.reconfigure ASfast @20:03:21.45 AS.seek 0ml @20:03:48.94 AFV.select :carrierSolution @20:03:49.08 delay 2 seconds @20:03:51.13 AS.reconfigure ASconfig @20:03:51.23 AS.seek 1ml @20:04:49.03 delay 1 second @20:04:50.05 AFV.select :holdingCoil @20:04:50.15 delay 1 second @20:04:51.18 AS.reconfigure ASfast @20:04:51.23 AS.seek 0ml @20:05:18.61 AFV.select :carrierSolution @20:05:18.72 delay 2 seconds @20:05:20.75 AS.reconfigure ASconfig @20:05:20.80 AS.seek 1ml @20:06:18.54 delay 1 second @20:06:19.56 AFV.select :holdingCoil @20:06:19.67 delay 1 second @20:06:20.70 AS.reconfigure ASfast @20:06:20.75 AS.seek 0ml @20:06:48.11 ARV.dial between carrier and air1 @20:06:48.61 ATV.dial :air3 @20:06:49.90 AS.reconfigure ASslow1 @20:06:49.96 AS.seek 0.01ml @20:06:52.25 delay 2 seconds @20:06:54.27 ATV.dial :bleach @20:06:55.31 AS.seek 0.81ml @20:09:36.46 delay 3 seconds @20:09:39.48 ATV.dial :air3 @20:09:40.04 delay 3 seconds @20:09:43.11 AS.seek 0.835ml @20:09:48.43 delay 3 seconds @20:09:51.45 ATV.dial :bleach @20:09:52.07 AS.seek 0.82ml @20:09:55.37 delay 3 seconds @20:09:58.39 ATV.dial PCR @20:09:58.79 AS.reconfigure ASconfig @20:09:58.85 AS.seek :empty @20:10:46.32 PCR.program :bleachProfile @20:10:46.38 PCR program should complete in < 2:45 @20:10:46.39 PCR.run 10:00 @20:13:42.17 PCR.off @20:13:42.20 AFV.select :carrierSolution @20:13:42.32 delay 2 seconds @20:13:44.35 AS.seek 1ml @20:14:42.18 delay 1 second @20:14:43.20 AFV.select :holdingCoil @20:14:43.30 delay 1 second @20:14:44.33 AS.reconfigure ASfast @20:14:44.38 AS.seek 0ml @20:15:11.95 delay 3 seconds @20:15:14.97 AFV.select :carrierSolution @20:15:15.07 delay 2 seconds @20:15:17.10 AS.reconfigure ASconfig @20:15:17.16 AS.seek 1ml @20:16:14.90 delay 1 second @20:16:15.92 AFV.select :holdingCoil @20:16:16.02 delay 1 second @20:16:17.05 AS.reconfigure ASfast @20:16:17.10 AS.seek 0ml @20:16:44.48 delay 3 seconds @20:16:47.50 AFV.select :carrierSolution @20:16:47.60 delay 2 seconds @20:16:49.63 AS.reconfigure ASconfig @20:16:49.69 AS.seek 1ml @20:17:47.43 delay 1 second @20:17:48.45 AFV.select :holdingCoil @20:17:48.55 delay 1 second @20:17:49.58 AS.reconfigure ASfast @20:17:49.63 AS.seek 0ml @20:18:16.98 delay 3 seconds @20:18:20.00 AFV.select :carrierSolution @20:18:20.10 delay 2 seconds @20:18:22.13 AS.reconfigure ASconfig @20:18:22.19 AS.seek 1ml @20:19:19.95 delay 1 second @20:19:20.97 AFV.select :holdingCoil @20:19:21.07 delay 1 second @20:19:22.10 AS.reconfigure ASfast @20:19:22.15 AS.seek 0ml @20:19:49.51 delay 3 seconds @20:19:52.53 AFV.select :carrierSolution @20:19:52.63 delay 2 seconds @20:19:54.66 AS.reconfigure ASconfig @20:19:54.72 AS.seek 1ml @20:20:52.48 delay 1 second @20:20:53.50 AFV.select :holdingCoil @20:20:53.60 delay 1 second @20:20:54.63 AS.reconfigure ASnorm @20:20:54.68 AS.seek 0ml @20:21:45.38
AFV.select :carrierSolution @20:21:45.49 delay 2 seconds @20:21:47.52 AS.reconfigure ASconfig @20:21:47.57 AS.seek 0.5ml @20:22:16.61 delay 1 second @20:22:17.63 AFV.select :holdingCoil @20:22:17.73 ATV.dial :air1 @20:22:19.24 AS.reconfigure ASslow2 @20:22:19.30 AS.seek 0.51ml @20:22:29.57 delay 5 seconds @20:22:34.59 ATV.dial :extSrc @20:22:36.31 AS.seek 0.53ml @20:22:56.75 delay 10 seconds @20:23:06.78 ATV.dial :air1 @20:23:08.49 AS.reconfigure ASslow1 @20:23:08.54 AS.seek 0.56ml @20:23:14.90 ATV.dial :waste @20:23:15.47 AS.reconfigure ASconfig @20:23:15.53 AS.seek :empty @20:23:48.23 Setting up PCR for NM -- writing /var/log/esp/NM20mar1720h.pcr @20:23:48.25 Building PCR Slug from extSrc with MM1 mix, PP1 primer @20:23:48.27 Priming with carrier @20:23:48.29 ATV.dial ARV @20:23:49.79 ARV.dial :carrier @20:23:50.15 AS.reconfigure ASnorm @20:23:50.21 AS.seek 0.499ml @20:24:15.79 AFV.select :carrierSolution @20:24:15.90 delay 2 seconds @20:24:17.93 AS.reconfigure ASconfig @20:24:17.99 AS.seek 1ml @20:24:47.07 delay 1 second @20:24:48.09 AFV.select :holdingCoil @20:24:48.19 delay 1 second @20:24:49.21 ATV.dial :waste @20:24:50.89 AS.reconfigure ASnorm @20:24:50.96 AS.seek :empty @20:25:41.72 AFV.select :carrierSolution @20:25:41.83 delay 2 seconds @20:25:43.86 AS.reconfigure ASconfig @20:25:43.91 AS.seek 0.5ml @20:26:13.04 delay 1 second @20:26:14.06 AFV.select :holdingCoil @20:26:14.17 Building Slug @20:26:14.18 ATV.dial :ARV @20:26:15.84 delay 2 seconds @20:26:17.86 ARV.dial :air1 @20:26:18.27 delay 2 seconds @20:26:20.34 AS.reconfigure ASslow2 @20:26:20.40 AS.seek 0.506ml @20:26:26.64 delay 2 seconds @20:26:28.66 ARV.dial :MM1 @20:26:29.36 delay 2 seconds @20:26:31.38 Adding MM1 @20:26:31.46 AS.seek 0.524ml @20:26:49.73 delay 25 seconds @20:27:15.15 ARV.dial :PP1 @20:27:15.53 Adding PP1 @20:27:15.54 delay 2 seconds @20:27:17.62 AS.seek 0.53ml @20:27:24.00 delay 10 seconds @20:27:34.03 ARV.dial :air1 @20:27:34.42 adding ARV air @20:27:34.43 delay 2 seconds @20:27:36.51 AS.seek 0.536ml @20:27:42.89 delay 5 seconds @20:27:47.91 ARV.dial :carrier @20:27:48.32 delay 7 seconds @20:27:55.40 AS.seek 0.594ml @20:28:54.12 delay 5 seconds @20:28:59.14 ATV.dial :extSrc @20:28:59.72 delay 2 seconds @20:29:01.79 AS.seek 0.6ml @20:29:08.23 delay 4 seconds @20:29:12.25 ATV.dial ARV @20:29:12.81 delay 2 seconds @20:29:14.83 ARV.dial :carrier @20:29:14.84 delay 2 seconds @20:29:16.92 AS.seek 0.65ml @20:30:07.32 delay 4 seconds @20:30:11.34 ATV.dial PCR @20:30:12.12 ASV.dial :waste1 @20:30:12.13 ARV.dial :air1 @20:30:12.54 Positioning Slug by 0.324ml @20:30:12.63 AS.seek 0.326ml @20:35:38.67 delay 5 seconds @20:35:43.69 ATV.dial ARV @20:35:44.48 ASV.dial :PCRlock @20:35:44.89 delay 5 seconds @20:35:49.91 Running NM Profile60C300 thermocycling @20:35:49.93 PCR.program Profile60C300 @20:35:50.04 Profile60C300 should complete in < 1:40:53 @20:35:50.05 PCR.run 2:11:06.25 @21:57:01.23 PCR.off @21:57:01.78 PCR for NM completed -- closed /var/log/esp/NM20mar1720h.pcr @21:57:01.85 Cleaning PCR @21:57:01.86 AFV.select :carrierSolution @21:57:01.98 delay 2 seconds @21:57:04.01 AS.reconfigure ASconfig @21:57:04.06 AS.seek 0.5ml @21:57:14.37 delay 1 second @21:57:15.39 AFV.select :holdingCoil @21:57:15.49 ASV.dial :waste1 @21:57:15.89 ATV.dial :air3 @21:57:17.18 AS.reconfigure ASslow1 @21:57:17.24 AS.seek 0.51ml @21:57:19.55 delay 2 seconds @21:57:21.57 ATV.dial :bleach @21:57:22.20 AS.seek 0.81ml @21:58:22.80 delay 3 seconds @21:58:25.82 ATV.dial :air3 @21:58:26.45 delay 3 seconds @21:58:29.52 AS.seek 0.835ml @21:58:34.82 delay 3 seconds @21:58:37.84 ATV.dial :bleach @21:58:38.47 AS.seek 0.82ml @21:58:41.77 delay 3 seconds @21:58:44.79 ARV.dial :waste @21:58:45.42 delay 3 seconds @21:58:48.44 ATV.dial :ARV @21:58:49.34 AS.reconfigure ASconfig @21:58:49.40 AS.seek 0.47ml @21:59:09.83 AFV.select :carrierSolution @21:59:09.94 delay 2 seconds @21:59:11.97 AS.seek 1ml @21:59:42.77 delay 1 second @21:59:43.79 AFV.select :holdingCoil @21:59:43.89 delay 1 second @21:59:44.92 AS.reconfigure ASfast @21:59:44.98 AS.seek 0ml @22:00:12.44 AFV.select :carrierSolution @22:00:12.55 delay 2 seconds @22:00:14.58 AS.reconfigure ASconfig @22:00:14.63 AS.seek 1ml @22:01:12.42 delay 1 second @22:01:13.44 AFV.select :holdingCoil @22:01:13.54 delay 1 second @22:01:14.57 AS.reconfigure ASfast @22:01:14.63 AS.seek 0ml @22:01:41.98 AFV.select :carrierSolution @22:01:42.09 delay 2 seconds @22:01:44.12 AS.reconfigure ASconfig @22:01:44.18 AS.seek 1ml @22:02:41.92 delay 1 second @22:02:42.94 AFV.select :holdingCoil @22:02:43.04 delay 1 second @22:02:44.07 AS.reconfigure ASfast @22:02:44.12 AS.seek 0ml @22:03:11.48 ARV.dial between carrier and air1 @22:03:11.98 ATV.dial :air3 @22:03:13.28 AS.reconfigure ASslow1 @22:03:13.34 AS.seek 0.01ml @22:03:15.70 delay 2 seconds @22:03:17.72 ATV.dial :bleach @22:03:18.34 AS.seek 0.81ml @22:05:59.50 delay 3 seconds @22:06:02.52 ATV.dial :air3 @22:06:03.08 delay 3 seconds @22:06:06.15 AS.seek 0.835ml @22:06:11.48 delay 3 seconds @22:06:14.50 ATV.dial :bleach @22:06:15.12 AS.seek 0.82ml @22:06:18.42 delay 3 seconds @22:06:21.44 ATV.dial PCR @22:06:21.84 AS.reconfigure ASconfig @22:06:21.90 AS.seek :empty @22:07:09.38 PCR.program :bleachProfile @22:07:09.44 PCR program should complete in < 2:45 @22:07:09.45 PCR.run 10:00 @22:10:05.23 PCR.off @22:10:05.26 AFV.select :carrierSolution @22:10:05.38 delay 2 seconds @22:10:07.41 AS.seek 1ml @22:11:05.23 delay 1 second @22:11:06.25 AFV.select :holdingCoil @22:11:06.35 delay 1 second @22:11:07.38 AS.reconfigure ASfast @22:11:07.43 AS.seek 0ml @22:11:34.80 delay 3 seconds @22:11:37.82 AFV.select :carrierSolution @22:11:37.92 delay 2 seconds @22:11:39.95 AS.reconfigure ASconfig @22:11:40.01 AS.seek 1ml @22:12:37.78 delay 1 second @22:12:38.80 AFV.select :holdingCoil @22:12:38.90 delay 1 second @22:12:39.93 AS.reconfigure ASfast @22:12:39.98 AS.seek 0ml @22:13:07.34 delay 3 seconds @22:13:10.36 AFV.select :carrierSolution @22:13:10.46 delay 2 seconds @22:13:12.49 AS.reconfigure ASconfig @22:13:12.54 AS.seek 1ml @22:14:10.31 delay 1 second @22:14:11.33 AFV.select :holdingCoil @22:14:11.43 delay 1 second @22:14:12.46 AS.reconfigure ASfast @22:14:12.51 AS.seek 0ml @22:14:39.88 delay 3 seconds @22:14:42.90 AFV.select :carrierSolution @22:14:43.00 delay 2 seconds @22:14:45.03 AS.reconfigure ASconfig @22:14:45.09 AS.seek 1ml @22:15:42.84 delay 1 second @22:15:43.86 AFV.select :holdingCoil @22:15:43.96 delay 1 second @22:15:44.99 AS.reconfigure ASfast @22:15:45.04 AS.seek 0ml @22:16:12.41 delay 3 seconds @22:16:15.43 AFV.select :carrierSolution @22:16:15.53 delay 2 seconds @22:16:17.56 AS.reconfigure ASconfig @22:16:18.01 AS.seek 1ml @22:17:15.77 delay 1 second @22:17:16.79 AFV.select :holdingCoil @22:17:16.89 delay 1 second @22:17:17.92 AS.reconfigure ASnorm @22:17:17.97 AS.seek 0ml @22:18:08.71
AFV.select :carrierSolution @22:18:08.82 delay 2 seconds @22:18:10.85 AS.reconfigure ASconfig @22:18:10.90 AS.seek 0.5ml @22:18:39.97 delay 1 second @22:18:40.99 AFV.select :holdingCoil @22:18:41.09 ATV.dial :air1 @22:18:42.59 AS.reconfigure ASslow2 @22:18:42.65 AS.seek 0.506ml @22:18:48.83 ATV.dial :extSrc @22:18:50.55 AS.seek 0.512ml @22:18:56.86 delay 5 seconds @22:19:01.88 ATV.dial :air1 @22:19:03.61 AS.reconfigure ASslow1 @22:19:03.67 AS.seek 0.542ml @22:19:10.03 ATV.dial :waste @22:19:10.59 AS.reconfigure ASconfig @22:19:10.65 AS.seek :empty @22:19:42.29 Setting up PCR for NM -- writing /var/log/esp/NM20mar1722h.pcr @22:19:42.32 Building PCR Slug from extSrc with MM1 mix, PP1 primer @22:19:42.34 Priming with carrier @22:19:42.36 ATV.dial ARV @22:19:43.86 ARV.dial :carrier @22:19:44.20 AS.reconfigure ASnorm @22:19:44.26 AS.seek 0.499ml @22:20:09.81 AFV.select :carrierSolution @22:20:09.92 delay 2 seconds @22:20:11.95 AS.reconfigure ASconfig @22:20:12.01 AS.seek 1ml @22:20:41.08 delay 1 second @22:20:42.10 AFV.select :holdingCoil @22:20:42.20 delay 1 second @22:20:43.22 ATV.dial :waste @22:20:44.89 AS.reconfigure ASnorm @22:20:44.96 AS.seek :empty @22:21:35.69 AFV.select :carrierSolution @22:21:35.81 delay 2 seconds @22:21:37.84 AS.reconfigure ASconfig @22:21:37.89 AS.seek 0.5ml @22:22:07.02 delay 1 second @22:22:08.04 AFV.select :holdingCoil @22:22:08.14 Building Slug @22:22:08.16 ATV.dial :ARV @22:22:09.80 delay 2 seconds @22:22:11.82 ARV.dial :air1 @22:22:12.23 delay 2 seconds @22:22:14.30 AS.reconfigure ASslow2 @22:22:14.36 AS.seek 0.506ml @22:22:20.56 delay 2 seconds @22:22:22.58 ARV.dial :MM1 @22:22:23.19 delay 2 seconds @22:22:25.21 Adding MM1 @22:22:25.30 AS.seek 0.524ml @22:22:43.67 delay 25 seconds @22:23:08.69 ARV.dial :PP1 @22:23:09.09 Adding PP1 @22:23:09.10 delay 2 seconds @22:23:11.18 AS.seek 0.53ml @22:23:17.83 delay 10 seconds @22:23:27.86 ARV.dial :air1 @22:23:28.25 adding ARV air @22:23:28.26 delay 2 seconds @22:23:30.34 AS.seek 0.536ml @22:23:36.72 delay 5 seconds @22:23:41.74 ARV.dial :carrier @22:23:42.14 delay 7 seconds @22:23:49.22 AS.seek 0.594ml @22:24:47.92 delay 5 seconds @22:24:52.94 ATV.dial :extSrc @22:24:53.52 delay 2 seconds @22:24:55.59 AS.seek 0.6ml @22:25:02.17 delay 4 seconds @22:25:06.19 ATV.dial ARV @22:25:06.74 delay 2 seconds @22:25:08.76 ARV.dial :carrier @22:25:08.77 delay 2 seconds @22:25:10.85 AS.seek 0.65ml @22:26:01.38 delay 4 seconds @22:26:05.40 ATV.dial PCR @22:26:06.19 ASV.dial :waste1 @22:26:06.20 ARV.dial :air1 @22:26:06.61 Positioning Slug by 0.324ml @22:26:06.71 AS.seek 0.326ml @22:31:32.95 delay 5 seconds @22:31:37.97 ATV.dial ARV @22:31:38.77 ASV.dial :PCRlock @22:31:39.17 delay 5 seconds @22:31:44.19 Running NM Profile60C300 thermocycling @22:31:44.21 PCR.program Profile60C300 @22:31:44.70 Profile60C300 should complete in < 1:40:53 @22:31:44.71 PCR.run 2:11:06.25 @23:52:52.06 PCR.off @23:52:52.61 PCR for NM completed -- closed /var/log/esp/NM20mar1722h.pcr @23:52:52.69 Cleaning PCR @23:52:52.70 AFV.select :carrierSolution @23:52:52.82 delay 2 seconds @23:52:54.85 AS.reconfigure ASconfig @23:52:54.90 AS.seek 0.5ml @23:53:05.21 delay 1 second @23:53:06.23 AFV.select :holdingCoil @23:53:06.33 ASV.dial :waste1 @23:53:06.74 ATV.dial :air3 @23:53:08.05 AS.reconfigure ASslow1 @23:53:08.11 AS.seek 0.51ml @23:53:10.42 delay 2 seconds @23:53:12.44 ATV.dial :bleach @23:53:13.06 AS.seek 0.81ml @23:54:13.64 delay 3 seconds @23:54:16.66 ATV.dial :air3 @23:54:17.24 delay 3 seconds @23:54:20.31 AS.seek 0.835ml @23:54:25.61 delay 3 seconds @23:54:28.63 ATV.dial :bleach @23:54:29.27 AS.seek 0.82ml @23:54:32.58 delay 3 seconds @23:54:35.60 ARV.dial :waste @23:54:36.21 delay 3 seconds @23:54:39.23 ATV.dial :ARV @23:54:40.22 AS.reconfigure ASconfig @23:54:40.28 AS.seek 0.47ml @23:55:00.69 AFV.select :carrierSolution @23:55:00.80 delay 2 seconds @23:55:02.83 AS.seek 1ml @23:55:33.60 delay 1 second @23:55:34.62 AFV.select :holdingCoil @23:55:34.72 delay 1 second @23:55:35.75 AS.reconfigure ASfast @23:55:35.80 AS.seek 0ml @23:56:03.18 AFV.select :carrierSolution @23:56:03.29 delay 2 seconds @23:56:05.32 AS.reconfigure ASconfig @23:56:05.37 AS.seek 1ml @23:57:03.18 delay 1 second @23:57:04.20 AFV.select :holdingCoil @23:57:04.30 delay 1 second @23:57:05.33 AS.reconfigure ASfast @23:57:05.38 AS.seek 0ml @23:57:32.97 AFV.select :carrierSolution @23:57:33.08 delay 2 seconds @23:57:35.11 AS.reconfigure ASconfig @23:57:35.16 AS.seek 1ml @23:58:32.91 delay 1 second @23:58:33.93 AFV.select :holdingCoil @23:58:34.03 delay 1 second @23:58:35.06 AS.reconfigure ASfast @23:58:35.11 AS.seek 0ml @23:59:02.47 ARV.dial between carrier and air1 @23:59:02.97 ATV.dial :air3 @23:59:04.28 AS.reconfigure ASslow1 @23:59:04.34 AS.seek 0.01ml @23:59:06.66 delay 2 seconds @23:59:08.68 ATV.dial :bleach @23:59:09.71 AS.seek 0.81ml @00:01:50.85CET18-Mar-20 delay 3 seconds @00:01:53.87 ATV.dial :air3 @00:01:54.44 delay 3 seconds @00:01:57.52 AS.seek 0.835ml @00:02:02.83 delay 3 seconds @00:02:05.85 ATV.dial :bleach @00:02:06.47 AS.seek 0.82ml @00:02:09.80 delay 3 seconds @00:02:12.82 ATV.dial PCR @00:02:13.22 AS.reconfigure ASconfig @00:02:13.28 AS.seek :empty @00:03:00.75 PCR.program :bleachProfile @00:03:00.81 PCR program should complete in < 2:45 @00:03:00.82 PCR.run 10:00 @00:05:59.63 PCR.off @00:05:59.66 AFV.select :carrierSolution @00:05:59.78 delay 2 seconds @00:06:01.81 AS.seek 1ml @00:06:59.68 delay 1 second @00:07:00.70 AFV.select :holdingCoil @00:07:00.80 delay 1 second @00:07:01.83 AS.reconfigure ASfast @00:07:01.88 AS.seek 0ml @00:07:29.25 delay 3 seconds @00:07:32.27 AFV.select :carrierSolution @00:07:32.37 delay 2 seconds @00:07:34.40 AS.reconfigure ASconfig @00:07:34.45 AS.seek 1ml @00:08:32.19 delay 1 second @00:08:33.21 AFV.select :holdingCoil @00:08:33.31 delay 1 second @00:08:34.34 AS.reconfigure ASfast @00:08:34.39 AS.seek 0ml @00:09:01.75 delay 3 seconds @00:09:04.77 AFV.select :carrierSolution @00:09:04.87 delay 2 seconds @00:09:06.90 AS.reconfigure ASconfig @00:09:06.96 AS.seek 1ml @00:10:04.71 delay 1 second @00:10:05.73 AFV.select :holdingCoil @00:10:05.83 delay 1 second @00:10:06.86 AS.reconfigure ASfast @00:10:06.91 AS.seek 0ml @00:10:34.27 delay 3 seconds @00:10:37.29 AFV.select :carrierSolution @00:10:37.39 delay 2 seconds @00:10:39.42 AS.reconfigure ASconfig @00:10:39.48 AS.seek 1ml @00:11:37.22 delay 1 second @00:11:38.24 AFV.select :holdingCoil @00:11:38.34 delay 1 second @00:11:39.37 AS.reconfigure ASfast @00:11:39.42 AS.seek 0ml @00:12:06.79 delay 3 seconds @00:12:09.81 AFV.select :carrierSolution @00:12:09.91 delay 2 seconds @00:12:11.94 AS.reconfigure ASconfig @00:12:12.00 AS.seek 1ml @00:13:09.75 delay 1 second @00:13:10.77 AFV.select :holdingCoil @00:13:10.87 delay 1 second @00:13:11.90 AS.reconfigure ASnorm @00:13:11.95 AS.seek 0ml @00:14:02.67
AFV.select :carrierSolution @00:14:02.78 delay 2 seconds @00:14:04.81 AS.reconfigure ASconfig @00:14:04.86 AS.seek 0.5ml @00:14:33.93 delay 1 second @00:14:34.95 AFV.select :holdingCoil @00:14:35.05 ATV.dial :air1 @00:14:36.55 AS.reconfigure ASslow1 @00:14:36.61 AS.seek 0.52ml @00:14:40.93 delay 5 seconds @00:14:45.95 ATV.dial :extSrc @00:14:47.67 AS.reconfigure ASslow2 @00:14:47.72 AS.seek 0.545ml @00:15:13.04 delay 10 seconds @00:15:23.06 ATV.dial :air1 @00:15:24.78 AS.reconfigure ASslow1 @00:15:24.83 AS.seek 0.575ml @00:15:31.19 ATV.dial :waste @00:15:31.76 AS.reconfigure ASconfig @00:15:31.82 AS.seek :empty @00:16:05.38 Setting up PCR for NM -- writing /var/log/esp/NM20mar1800h.pcr @00:16:05.41 Building PCR Slug from extSrc with MM1 mix, PP1 primer @00:16:05.43 Priming with carrier @00:16:05.45 ATV.dial ARV @00:16:06.94 ARV.dial :carrier @00:16:07.28 AS.reconfigure ASnorm @00:16:07.34 AS.seek 0.499ml @00:16:32.93 AFV.select :carrierSolution @00:16:33.04 delay 2 seconds @00:16:35.07 AS.reconfigure ASconfig @00:16:35.13 AS.seek 1ml @00:17:04.18 delay 1 second @00:17:05.20 AFV.select :holdingCoil @00:17:05.30 delay 1 second @00:17:06.32 ATV.dial :waste @00:17:07.99 AS.reconfigure ASnorm @00:17:08.43 AS.seek :empty @00:17:59.18 AFV.select :carrierSolution @00:17:59.30 delay 2 seconds @00:18:01.33 AS.reconfigure ASconfig @00:18:01.39 AS.seek 0.5ml @00:18:30.50 delay 1 second @00:18:31.52 AFV.select :holdingCoil @00:18:31.62 Building Slug @00:18:31.64 ATV.dial :ARV @00:18:33.29 delay 2 seconds @00:18:35.31 ARV.dial :air1 @00:18:35.70 delay 2 seconds @00:18:37.77 AS.reconfigure ASslow2 @00:18:37.84 AS.seek 0.506ml @00:18:44.11 delay 2 seconds @00:18:46.13 ARV.dial :MM1 @00:18:46.74 delay 2 seconds @00:18:48.76 Adding MM1 @00:18:48.84 AS.seek 0.524ml @00:19:07.13 delay 25 seconds @00:19:32.15 ARV.dial :PP1 @00:19:32.55 Adding PP1 @00:19:32.56 delay 2 seconds @00:19:34.65 AS.seek 0.53ml @00:19:40.93 delay 10 seconds @00:19:50.96 ARV.dial :air1 @00:19:51.35 adding ARV air @00:19:51.36 delay 2 seconds @00:19:53.45 AS.seek 0.536ml @00:19:59.91 delay 5 seconds @00:20:04.93 ARV.dial :carrier @00:20:05.33 delay 7 seconds @00:20:12.41 AS.seek 0.594ml @00:21:11.13 delay 5 seconds @00:21:16.15 ATV.dial :extSrc @00:21:16.72 delay 2 seconds @00:21:18.79 AS.seek 0.6ml @00:21:25.02 delay 4 seconds @00:21:29.04 ATV.dial ARV @00:21:29.60 delay 2 seconds @00:21:31.62 ARV.dial :carrier @00:21:31.63 delay 2 seconds @00:21:33.71 AS.seek 0.65ml @00:22:24.11 delay 4 seconds @00:22:28.13 ATV.dial PCR @00:22:28.90 ASV.dial :waste1 @00:22:28.91 ARV.dial :air1 @00:22:29.33 Positioning Slug by 0.324ml @00:22:29.42 AS.seek 0.326ml @00:27:55.44 delay 5 seconds @00:28:00.46 ATV.dial ARV @00:28:01.26 ASV.dial :PCRlock @00:28:01.66 delay 5 seconds @00:28:06.69 Running NM Profile60C300 thermocycling @00:28:06.71 PCR.program Profile60C300 @00:28:06.81 Profile60C300 should complete in < 1:40:53 @00:28:06.82 PCR.run 2:11:06.25 @01:49:11.73 PCR.off @01:49:12.51 PCR for NM completed -- closed /var/log/esp/NM20mar1800h.pcr @01:49:12.59 Cleaning PCR @01:49:12.60 AFV.select :carrierSolution @01:49:12.72 delay 2 seconds @01:49:14.75 AS.reconfigure ASconfig @01:49:14.81 AS.seek 0.5ml @01:49:25.10 delay 1 second @01:49:26.12 AFV.select :holdingCoil @01:49:26.22 ASV.dial :waste1 @01:49:26.63 ATV.dial :air3 @01:49:27.94 AS.reconfigure ASslow1 @01:49:28.00 AS.seek 0.51ml @01:49:30.30 delay 2 seconds @01:49:32.32 ATV.dial :bleach @01:49:32.96 AS.seek 0.81ml @01:50:33.60 delay 3 seconds @01:50:36.62 ATV.dial :air3 @01:50:37.18 delay 3 seconds @01:50:40.25 AS.seek 0.835ml @01:50:45.57 delay 3 seconds @01:50:48.59 ATV.dial :bleach @01:50:49.21 AS.seek 0.82ml @01:50:52.51 delay 3 seconds @01:50:55.53 ARV.dial :waste @01:50:56.13 delay 3 seconds @01:50:59.15 ATV.dial :ARV @01:51:00.05 AS.reconfigure ASconfig @01:51:00.11 AS.seek 0.47ml @01:51:20.51 AFV.select :carrierSolution @01:51:20.62 delay 2 seconds @01:51:22.65 AS.seek 1ml @01:51:53.43 delay 1 second @01:51:54.45 AFV.select :holdingCoil @01:51:54.55 delay 1 second @01:51:55.58 AS.reconfigure ASfast @01:51:55.63 AS.seek 0ml @01:52:22.98 AFV.select :carrierSolution @01:52:23.09 delay 2 seconds @01:52:25.12 AS.reconfigure ASconfig @01:52:25.17 AS.seek 1ml @01:53:22.91 delay 1 second @01:53:23.93 AFV.select :holdingCoil @01:53:24.03 delay 1 second @01:53:25.06 AS.reconfigure ASfast @01:53:25.11 AS.seek 0ml @01:53:52.56 AFV.select :carrierSolution @01:53:52.67 delay 2 seconds @01:53:54.70 AS.reconfigure ASconfig @01:53:54.75 AS.seek 1ml @01:54:52.52 delay 1 second @01:54:53.54 AFV.select :holdingCoil @01:54:53.64 delay 1 second @01:54:54.67 AS.reconfigure ASfast @01:54:54.72 AS.seek 0ml @01:55:22.07 ARV.dial between carrier and air1 @01:55:22.57 ATV.dial :air3 @01:55:23.87 AS.reconfigure ASslow1 @01:55:23.93 AS.seek 0.01ml @01:55:26.23 delay 2 seconds @01:55:28.25 ATV.dial :bleach @01:55:28.87 AS.seek 0.81ml @01:58:10.01 delay 3 seconds @01:58:13.03 ATV.dial :air3 @01:58:13.59 delay 3 seconds @01:58:16.66 AS.seek 0.835ml @01:58:21.99 delay 3 seconds @01:58:25.01 ATV.dial :bleach @01:58:25.63 AS.seek 0.82ml @01:58:28.95 delay 3 seconds @01:58:31.97 ATV.dial PCR @01:58:32.38 AS.reconfigure ASconfig @01:58:32.44 AS.seek :empty @01:59:19.95 PCR.program :bleachProfile @01:59:20.05 PCR program should complete in < 2:45 @01:59:20.07 PCR.run 10:00 @02:02:18.91 PCR.off @02:02:18.94 AFV.select :carrierSolution @02:02:19.05 delay 2 seconds @02:02:21.08 AS.seek 1ml @02:03:18.87 delay 1 second @02:03:19.89 AFV.select :holdingCoil @02:03:19.99 delay 1 second @02:03:21.02 AS.reconfigure ASfast @02:03:21.07 AS.seek 0ml @02:03:48.65 delay 3 seconds @02:03:51.68 AFV.select :carrierSolution @02:03:51.81 delay 2 seconds @02:03:53.86 AS.reconfigure ASconfig @02:03:53.96 AS.seek 1ml @02:04:51.77 delay 1 second @02:04:52.79 AFV.select :holdingCoil @02:04:52.89 delay 1 second @02:04:53.92 AS.reconfigure ASfast @02:04:54.04 AS.seek 0ml @02:05:21.38 delay 3 seconds @02:05:24.40 AFV.select :carrierSolution @02:05:24.50 delay 2 seconds @02:05:26.53 AS.reconfigure ASconfig @02:05:26.58 AS.seek 1ml @02:06:24.35 delay 1 second @02:06:25.37 AFV.select :holdingCoil @02:06:25.48 delay 1 second @02:06:26.51 AS.reconfigure ASfast @02:06:26.56 AS.seek 0ml @02:06:53.92 delay 3 seconds @02:06:56.94 AFV.select :carrierSolution @02:06:57.04 delay 2 seconds @02:06:59.07 AS.reconfigure ASconfig @02:06:59.13 AS.seek 1ml @02:07:56.96 delay 1 second @02:07:57.98 AFV.select :holdingCoil @02:07:58.08 delay 1 second @02:07:59.11 AS.reconfigure ASfast @02:07:59.16 AS.seek 0ml @02:08:26.51 delay 3 seconds @02:08:29.53 AFV.select :carrierSolution @02:08:29.63 delay 2 seconds @02:08:31.66 AS.reconfigure ASconfig @02:08:31.73 AS.seek 1ml @02:09:29.49 delay 1 second @02:09:30.51 AFV.select :holdingCoil @02:09:30.61 delay 1 second @02:09:31.64 AS.reconfigure ASnorm @02:09:31.69 AS.seek 0ml @02:10:22.39
AFV.select :carrierSolution @02:10:22.50 delay 2 seconds @02:10:24.53 AS.reconfigure ASconfig @02:10:24.58 AS.seek 0.5ml @02:10:53.60 delay 1 second @02:10:54.62 AFV.select :holdingCoil @02:10:54.72 ATV.dial :air1 @02:10:56.23 AS.reconfigure ASslow2 @02:10:56.29 AS.seek 0.506ml @02:11:02.51 ATV.dial :extSrc @02:11:04.23 AS.seek 0.512ml @02:11:10.52 delay 5 seconds @02:11:15.54 ATV.dial :air1 @02:11:17.26 AS.reconfigure ASslow1 @02:11:17.32 AS.seek 0.542ml @02:11:23.60 ATV.dial :waste @02:11:24.17 AS.reconfigure ASconfig @02:11:24.23 AS.seek :empty @02:11:55.90 Setting up PCR for NM -- writing /var/log/esp/NM20mar1802h.pcr @02:11:55.93 Building PCR Slug from extSrc with MM1 mix, PP1 primer @02:11:55.95 Priming with carrier @02:11:55.97 ATV.dial ARV @02:11:57.46 ARV.dial :carrier @02:11:57.82 AS.reconfigure ASnorm @02:11:57.88 AS.seek 0.499ml @02:12:23.43 AFV.select :carrierSolution @02:12:23.55 delay 2 seconds @02:12:25.58 AS.reconfigure ASconfig @02:12:25.64 AS.seek 1ml @02:12:54.71 delay 1 second @02:12:55.73 AFV.select :holdingCoil @02:12:55.83 delay 1 second @02:12:56.85 ATV.dial :waste @02:12:58.53 AS.reconfigure ASnorm @02:12:58.60 AS.seek :empty @02:13:49.34 AFV.select :carrierSolution @02:13:49.46 delay 2 seconds @02:13:51.49 AS.reconfigure ASconfig @02:13:51.54 AS.seek 0.5ml @02:14:20.63 delay 1 second @02:14:21.65 AFV.select :holdingCoil @02:14:21.76 Building Slug @02:14:21.77 ATV.dial :ARV @02:14:23.42 delay 2 seconds @02:14:25.44 ARV.dial :air1 @02:14:25.86 delay 2 seconds @02:14:27.93 AS.reconfigure ASslow2 @02:14:27.99 AS.seek 0.506ml @02:14:34.34 delay 2 seconds @02:14:36.36 ARV.dial :MM1 @02:14:36.96 delay 2 seconds @02:14:38.98 Adding MM1 @02:14:39.07 AS.seek 0.524ml @02:14:57.68 delay 25 seconds @02:15:22.70 ARV.dial :PP1 @02:15:23.09 Adding PP1 @02:15:23.10 delay 2 seconds @02:15:25.18 AS.seek 0.53ml @02:15:31.77 delay 10 seconds @02:15:41.80 ARV.dial :air1 @02:15:42.20 adding ARV air @02:15:42.21 delay 2 seconds @02:15:44.29 AS.seek 0.536ml @02:15:50.63 delay 5 seconds @02:15:55.65 ARV.dial :carrier @02:15:56.06 delay 7 seconds @02:16:03.14 AS.seek 0.594ml @02:17:01.98 delay 5 seconds @02:17:07.00 ATV.dial :extSrc @02:17:07.57 delay 2 seconds @02:17:09.64 AS.seek 0.6ml @02:17:16.12 delay 4 seconds @02:17:20.14 ATV.dial ARV @02:17:21.09 delay 2 seconds @02:17:23.11 ARV.dial :carrier @02:17:23.12 delay 2 seconds @02:17:25.21 AS.seek 0.65ml @02:18:15.62 delay 4 seconds @02:18:19.64 ATV.dial PCR @02:18:20.42 ASV.dial :waste1 @02:18:20.43 ARV.dial :air1 @02:18:20.85 Positioning Slug by 0.324ml @02:18:20.95 AS.seek 0.326ml @02:23:47.28 delay 5 seconds @02:23:52.30 ATV.dial ARV @02:23:53.09 ASV.dial :PCRlock @02:23:53.51 delay 5 seconds @02:23:58.53 Running NM Profile60C300 thermocycling @02:23:58.55 PCR.program Profile60C300 @02:23:58.66 Profile60C300 should complete in < 1:40:53 @02:23:58.67 PCR.run 2:11:06.25 @03:44:27.33 Session Ended @03:45:11.13 PCR.off @03:45:11.67 PCR for NM completed -- closed /var/log/esp/NM20mar1802h.pcr @03:45:11.73 Cleaning PCR @03:45:11.74 AFV.select :carrierSolution @03:45:11.84 delay 2 seconds @03:45:13.87 AS.reconfigure ASconfig @03:45:13.91 AS.seek 0.5ml @03:45:24.20 delay 1 second @03:45:25.22 AFV.select :holdingCoil @03:45:25.31 ASV.dial :waste1 @03:45:25.70 ATV.dial :air3 @03:45:26.98 AS.reconfigure ASslow1 @03:45:27.02 AS.seek 0.51ml @03:45:29.34 delay 2 seconds @03:45:31.36 ATV.dial :bleach @03:45:31.96 AS.seek 0.81ml @03:46:32.54 delay 3 seconds @03:46:35.56 ATV.dial :air3 @03:46:36.11 delay 3 seconds @03:46:39.18 AS.seek 0.835ml @03:46:44.47 delay 3 seconds @03:46:47.49 ATV.dial :bleach @03:46:48.09 AS.seek 0.82ml @03:46:51.37 delay 3 seconds @03:46:54.39 ARV.dial :waste @03:46:54.98 delay 3 seconds @03:46:58.00 ATV.dial :ARV @03:46:58.89 AS.reconfigure ASconfig @03:46:58.93 AS.seek 0.47ml @03:47:19.34 AFV.select :carrierSolution @03:47:19.44 delay 2 seconds @03:47:21.47 AS.seek 1ml @03:47:52.25 delay 1 second @03:47:53.27 AFV.select :holdingCoil @03:47:53.37 delay 1 second @03:47:54.40 AS.reconfigure ASfast @03:47:54.44 AS.seek 0ml @03:48:21.78 AFV.select :carrierSolution @03:48:21.88 delay 2 seconds @03:48:23.91 AS.reconfigure ASconfig @03:48:24.35 AS.seek 1ml @03:49:22.11 delay 1 second @03:49:23.13 AFV.select :holdingCoil @03:49:23.23 delay 1 second @03:49:24.26 AS.reconfigure ASfast @03:49:24.30 AS.seek 0ml @03:49:51.65 AFV.select :carrierSolution @03:49:51.75 delay 2 seconds @03:49:53.78 AS.reconfigure ASconfig @03:49:53.82 AS.seek 1ml @03:50:51.56 delay 1 second @03:50:52.58 AFV.select :holdingCoil @03:50:52.68 delay 1 second @03:50:53.71 AS.reconfigure ASfast @03:50:53.75 AS.seek 0ml @03:51:21.11 ARV.dial between carrier and air1 @03:51:21.59 ATV.dial :air3 @03:51:22.87 AS.reconfigure ASslow1 @03:51:22.91 AS.seek 0.01ml @03:51:25.18 delay 2 seconds @03:51:27.20 ATV.dial :bleach @03:51:27.81 AS.seek 0.81ml @03:54:08.93 delay 3 seconds @03:54:11.95 ATV.dial :air3 @03:54:12.50 delay 3 seconds @03:54:15.57 AS.seek 0.835ml @03:54:20.87 delay 3 seconds @03:54:23.89 ATV.dial :bleach @03:54:24.50 AS.seek 0.82ml @03:54:27.79 delay 3 seconds @03:54:30.81 ATV.dial PCR @03:54:31.20 AS.reconfigure ASconfig @03:54:31.25 AS.seek :empty @03:55:18.70 PCR.program :bleachProfile @03:55:18.75 PCR program should complete in < 2:45 @03:55:18.76 PCR.run 10:00 @03:58:14.53 PCR.off @03:58:14.56 AFV.select :carrierSolution @03:58:14.66 delay 2 seconds @03:58:16.69 AS.seek 1ml @03:59:14.51 delay 1 second @03:59:15.53 AFV.select :holdingCoil @03:59:15.63 delay 1 second @03:59:16.66 AS.reconfigure ASfast @03:59:16.70 AS.seek 0ml @03:59:44.03 delay 3 seconds @03:59:47.05 AFV.select :carrierSolution @03:59:47.15 delay 2 seconds @03:59:49.18 AS.reconfigure ASconfig @03:59:49.23 AS.seek 1ml @04:00:46.97 delay 1 second @04:00:47.99 AFV.select :holdingCoil @04:00:48.09 delay 1 second @04:00:49.12 AS.reconfigure ASfast @04:00:49.16 AS.seek 0ml @04:01:16.50 delay 3 seconds @04:01:19.52 AFV.select :carrierSolution @04:01:19.62 delay 2 seconds @04:01:21.65 AS.reconfigure ASconfig @04:01:21.70 AS.seek 1ml @04:02:19.45 delay 1 second @04:02:20.47 AFV.select :holdingCoil @04:02:20.57 delay 1 second @04:02:21.60 AS.reconfigure ASfast @04:02:21.64 AS.seek 0ml @04:02:48.98 delay 3 seconds @04:02:52.00 AFV.select :carrierSolution @04:02:52.10 delay 2 seconds @04:02:54.13 AS.reconfigure ASconfig @04:02:54.18 AS.seek 1ml @04:03:51.92 delay 1 second @04:03:52.94 AFV.select :holdingCoil @04:03:53.04 delay 1 second @04:03:54.07 AS.reconfigure ASfast @04:03:54.11 AS.seek 0ml @04:04:21.45 delay 3 seconds @04:04:24.47 AFV.select :carrierSolution @04:04:24.57 delay 2 seconds @04:04:26.60 AS.reconfigure ASconfig @04:04:26.65 AS.seek 1ml @04:05:24.40 delay 1 second @04:05:25.42 AFV.select :holdingCoil @04:05:25.52 delay 1 second @04:05:26.55 AS.reconfigure ASnorm @04:05:26.59 AS.seek 0ml @04:06:17.32
AFV.select :carrierSolution @04:06:17.42 delay 2 seconds @04:06:19.45 AS.reconfigure ASconfig @04:06:19.49 AS.seek 0.5ml @04:06:48.53 delay 1 second @04:06:49.55 AFV.select :holdingCoil @04:06:49.64 ATV.dial :air1 @04:06:51.14 AS.reconfigure ASslow1 @04:06:51.18 AS.seek 0.52ml @04:06:55.51 delay 5 seconds @04:07:00.53 ATV.dial :extSrc @04:07:02.25 AS.reconfigure ASslow2 @04:07:02.29 AS.seek 0.538ml @04:07:20.79 delay 10 seconds @04:07:30.81 ATV.dial :air1 @04:07:32.50 AS.reconfigure ASslow1 @04:07:32.55 AS.seek 0.568ml @04:07:38.84 ATV.dial :waste @04:07:39.39 AS.reconfigure ASconfig @04:07:39.43 AS.seek :empty @04:08:12.81 deconEluateRecoveryLine extSrc @04:08:12.82 ATV.dial :waste @04:08:12.83 AS.reconfigure ASnorm @04:08:12.88 AS.seek 0ml @04:08:13.23 ATV.dial :air3 @04:08:13.90 AS.reconfigure ASslow1 @04:08:13.94 AS.seek 0.01ml @04:08:16.20 delay 2 seconds @04:08:18.22 ATV.dial :bleach @04:08:18.68 AS.seek 0.31ml @04:09:19.37 delay 3 seconds @04:09:22.39 ATV.dial :air3 @04:09:22.95 delay 3 seconds @04:09:26.02 AS.seek 0.335ml @04:09:31.38 delay 3 seconds @04:09:34.40 ATV.dial :bleach @04:09:35.01 AS.seek 0.32ml @04:09:38.31 delay 3 seconds @04:09:41.33 ATV.dial :extSrc @04:09:41.95 AS.seek 0ml @04:10:46.56 AFV.select :carrierSolution @04:10:46.66 delay 2 seconds @04:10:48.69 AS.reconfigure ASconfig @04:10:48.73 AS.seek 0.5ml @04:11:17.75 delay 1 second @04:11:18.77 AFV.select :holdingCoil @04:11:18.87 ATV.dial :extSrc @04:11:18.89 AS.reconfigure ASslow1 @04:11:18.93 AS.seek 0ml @04:12:59.73 AFV.select :carrierSolution @04:12:59.83 delay 2 seconds @04:13:01.86 AS.reconfigure ASconfig @04:13:01.90 AS.seek 0.5ml @04:13:30.94 delay 1 second @04:13:31.96 AFV.select :holdingCoil @04:13:32.06 ATV.dial :extSrc @04:13:32.08 AS.reconfigure ASslow1 @04:13:32.12 AS.seek 0ml @04:15:12.90 AFV.select :carrierSolution @04:15:13.00 delay 2 seconds @04:15:15.03 AS.reconfigure ASconfig @04:15:15.07 AS.seek 0.5ml @04:15:44.11 delay 1 second @04:15:45.13 AFV.select :holdingCoil @04:15:45.23 ATV.dial :extSrc @04:15:45.25 AS.reconfigure ASslow1 @04:15:45.29 AS.seek 0ml @04:17:26.11 AFV.select :carrierSolution @04:17:26.21 delay 2 seconds @04:17:28.24 AS.reconfigure ASconfig @04:17:28.28 AS.seek 0.5ml @04:17:57.31 delay 1 second @04:17:58.33 AFV.select :holdingCoil @04:17:58.43 ATV.dial :extSrc @04:17:58.45 AS.reconfigure ASslow1 @04:17:58.49 AS.seek 0ml @04:19:39.31 ATV.dial :air2 @04:19:40.62 AS.reconfigure ASslow0 @04:19:40.66 AS.seek 0.5ml @04:20:31.30 ATV.dial :extSrc @04:20:32.76 AS.seek 0.02ml @04:21:21.36 delay 1 second @04:21:22.38 ATV.dial :waste @04:21:23.64 AS.reconfigure ASconfig @04:21:23.68 AS.seek :empty @04:21:25.19 deconEluateRecoveryLine extSrc @04:21:25.20 ATV.dial :waste @04:21:25.21 AS.reconfigure ASnorm @04:21:25.25 AS.seek 0ml @04:21:25.59 ATV.dial :air3 @04:21:26.43 AS.reconfigure ASslow1 @04:21:26.47 AS.seek 0.01ml @04:21:28.75 delay 2 seconds @04:21:30.77 ATV.dial :bleach @04:21:31.21 AS.seek 0.31ml @04:22:31.78 delay 3 seconds @04:22:34.80 ATV.dial :air3 @04:22:35.36 delay 3 seconds @04:22:38.43 AS.seek 0.335ml @04:22:43.64 delay 3 seconds @04:22:46.66 ATV.dial :bleach @04:22:47.26 AS.seek 0.32ml @04:22:50.55 delay 3 seconds @04:22:53.57 ATV.dial :extSrc @04:22:54.20 AS.seek 0ml @04:23:58.81 AFV.select :carrierSolution @04:23:58.91 delay 2 seconds @04:24:00.94 AS.reconfigure ASconfig @04:24:00.98 AS.seek 0.5ml @04:24:30.01 delay 1 second @04:24:31.03 AFV.select :holdingCoil @04:24:31.13 ATV.dial :extSrc @04:24:31.15 AS.reconfigure ASslow1 @04:24:31.19 AS.seek 0ml @04:26:12.03 AFV.select :carrierSolution @04:26:12.13 delay 2 seconds @04:26:14.16 AS.reconfigure ASconfig @04:26:14.20 AS.seek 0.5ml @04:26:43.22 delay 1 second @04:26:44.24 AFV.select :holdingCoil @04:26:44.34 ATV.dial :extSrc @04:26:44.36 AS.reconfigure ASslow1 @04:26:44.40 AS.seek 0ml @04:28:25.25 AFV.select :carrierSolution @04:28:25.35 delay 2 seconds @04:28:27.38 AS.reconfigure ASconfig @04:28:27.42 AS.seek 0.5ml @04:28:56.42 delay 1 second @04:28:57.44 AFV.select :holdingCoil @04:28:57.54 ATV.dial :extSrc @04:28:57.56 AS.reconfigure ASslow1 @04:28:57.60 AS.seek 0ml @04:30:38.45 AFV.select :carrierSolution @04:30:38.55 delay 2 seconds @04:30:40.58 AS.reconfigure ASconfig @04:30:40.97 AS.seek 0.5ml @04:31:10.00 delay 1 second @04:31:11.02 AFV.select :holdingCoil @04:31:11.12 ATV.dial :extSrc @04:31:11.14 AS.reconfigure ASslow1 @04:31:11.18 AS.seek 0ml @04:32:52.01 ATV.dial :air2 @04:32:53.32 AS.reconfigure ASslow0 @04:32:53.36 AS.seek 0.5ml @04:33:44.00 ATV.dial :extSrc @04:33:45.48 AS.seek 0.02ml @04:34:34.08 delay 1 second @04:34:35.10 ATV.dial :waste @04:34:36.37 AS.reconfigure ASconfig @04:34:36.41 AS.seek :empty @04:34:37.89 deconEluateRecoveryLine extSrc @04:34:37.90 ATV.dial :waste @04:34:37.91 AS.reconfigure ASnorm @04:34:37.96 AS.seek 0ml @04:34:38.31 ATV.dial :air3 @04:34:39.15 AS.reconfigure ASslow1 @04:34:39.19 AS.seek 0.01ml @04:34:41.44 delay 2 seconds @04:34:43.46 ATV.dial :bleach @04:34:43.90 AS.seek 0.31ml @04:35:44.50 delay 3 seconds @04:35:47.52 ATV.dial :air3 @04:35:48.08 delay 3 seconds @04:35:51.15 AS.seek 0.335ml @04:35:56.47 delay 3 seconds @04:35:59.49 ATV.dial :bleach @04:36:00.11 AS.seek 0.32ml @04:36:03.39 delay 3 seconds @04:36:06.41 ATV.dial :extSrc @04:36:07.02 AS.seek 0ml @04:37:11.64 AFV.select :carrierSolution @04:37:11.74 delay 2 seconds @04:37:13.77 AS.reconfigure ASconfig @04:37:13.82 AS.seek 0.5ml @04:37:42.84 delay 1 second @04:37:43.86 AFV.select :holdingCoil @04:37:43.96 ATV.dial :extSrc @04:37:43.98 AS.reconfigure ASslow1 @04:37:44.02 AS.seek 0ml @04:39:24.86 AFV.select :carrierSolution @04:39:24.96 delay 2 seconds @04:39:26.99 AS.reconfigure ASconfig @04:39:27.03 AS.seek 0.5ml @04:39:56.03 delay 1 second @04:39:57.05 AFV.select :holdingCoil @04:39:57.15 ATV.dial :extSrc @04:39:57.17 AS.reconfigure ASslow1 @04:39:57.21 AS.seek 0ml @04:41:38.05 AFV.select :carrierSolution @04:41:38.15 delay 2 seconds @04:41:40.18 AS.reconfigure ASconfig @04:41:40.22 AS.seek 0.5ml @04:42:09.25 delay 1 second @04:42:10.27 AFV.select :holdingCoil @04:42:10.37 ATV.dial :extSrc @04:42:10.39 AS.reconfigure ASslow1 @04:42:10.43 AS.seek 0ml @04:43:51.25 AFV.select :carrierSolution @04:43:51.35 delay 2 seconds @04:43:53.38 AS.reconfigure ASconfig @04:43:53.42 AS.seek 0.5ml @04:44:22.45 delay 1 second @04:44:23.47 AFV.select :holdingCoil @04:44:23.57 ATV.dial :extSrc @04:44:23.59 AS.reconfigure ASslow1 @04:44:23.63 AS.seek 0ml @04:46:04.45 ATV.dial :air2 @04:46:05.76 AS.reconfigure ASslow0 @04:46:05.80 AS.seek 0.5ml @04:46:56.37 ATV.dial :extSrc @04:46:57.84 AS.seek 0.02ml @04:47:46.44 delay 1 second @04:47:47.46 ATV.dial :waste @04:47:48.73 AS.reconfigure ASconfig @04:47:48.77 AS.seek :empty @15:57:11.48CET18-Mar-20
ESP2 v3.30 I2C Gateway v3.06 for core bus @15:57:13.32 Copyright 2018 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @15:57:13.83 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @15:57:19.38 Polling Can every 10:00 @15:57:21.49 SPE.coast @15:57:21.59 SPE.configure SPEconfig @15:57:21.69 AS.reconfigure ASconfig @15:57:21.73 AS.hold @15:57:21.78 AS.configure ASconfig @15:57:21.87 SS.reconfigure SSconfig @15:57:21.90 SS.hold @15:57:21.96 SS.configure SSconfig @15:57:22.05 CC.reconfigure CCconfig @15:57:22.09 CC.hold @15:57:22.16 CC.configure CCconfig @15:57:22.24 CS.reconfigure CSconfig @15:57:22.28 CS.hold @15:57:22.39 CS.configure CSconfig @15:57:23.16 PC.reconfigure PCconfig @15:57:23.20 PC.hold @15:57:23.24 PC.configure PCconfig @15:57:23.32 PS.configure PSconfig @15:57:23.42 Forearm.reconfigure LooseForearm @15:57:23.46 Forearm.hold @15:57:23.51 Forearm.configure LooseForearm @15:57:23.60 Elbow.reconfigure LooseElbow @15:57:23.64 Elbow.hold @15:57:23.68 Elbow.configure LooseElbow @15:57:23.78 SC.reconfigure SCloose @15:57:23.82 SC.hold @15:57:23.88 SC.configure SCloose @15:57:24.02 SE.reconfigure SEconfig @15:57:24.09 SE.hold @15:57:24.19 SE.configure SEconfig @15:57:24.26 CH.coast @15:57:24.37 CH.configure CHconfig @15:57:24.45 PH.coast @15:57:24.55 PH.configure PHconfig @15:57:24.62 PCR.off @15:57:24.66 PCR.pidtemp= 50,0.1,0,300,25,25 @15:57:24.69 PCR.caltemp= 2.8814,0.0372,0 @15:57:24.74 All dwarf controllers running firmware version 3.75 @15:57:24.88 Cleanly exited at 15:56:27CET18-Mar-20 @15:57:24.91 Loading /home/esp/esp2/mission/stdMER2017.rb mission @15:57:25.44 AFV.select :carrierSolution @15:57:25.55 delay 2 seconds @15:57:26.01 Session Begun @15:57:27.59
AS.seek 0.5ml @15:57:56.71 delay 1 second @15:57:57.73 AFV.select :holdingCoil @15:57:57.85 ATV.dial :extSrc @15:57:59.31 AS.reconfigure ASslow1 @15:57:59.37 AS.seek 0.53ml @15:58:05.69 delay 5 seconds @15:58:10.77 AS.reconfigure ASslow2 @15:58:10.83 AS.seek 0.54ml @15:58:21.05 delay 20 seconds @15:58:41.13 AS.reconfigure ASslow1 @15:58:41.19 AS.seek 0.57ml @15:58:47.49 delay 5 seconds @15:58:52.57 AS.reconfigure ASslow2 @15:58:52.63 AS.seek 0.58ml @15:59:02.91 delay 20 seconds @15:59:22.98 AS.reconfigure ASslow1 @15:59:23.04 AS.seek 0.61ml @15:59:29.42 delay 5 seconds @15:59:34.50 AS.reconfigure ASslow2 @15:59:34.56 AS.seek 0.62ml @15:59:44.94 delay 20 seconds @16:00:05.02 AS.reconfigure ASslow1 @16:00:05.09 AS.seek 0.65ml @16:00:11.42 delay 5 seconds @16:00:16.50 AS.reconfigure ASslow2 @16:00:16.56 AS.seek 0.66ml @16:00:27.00 delay 20 seconds @16:00:47.04 AS.reconfigure ASslow1 @16:00:47.10 AS.seek 0.705ml @16:00:56.41 delay 10 seconds @16:01:06.43 ATV.dial :air1 @16:01:08.14 AS.seek 0.725ml @16:01:12.42 delay 3 seconds @16:01:15.44 ATV.dial :waste @16:01:16.01 AS.reconfigure ASslow0 @16:01:16.06 AS.seek :empty @16:02:29.49 Setting up PCR for OM -- writing /var/log/esp/OM20mar1816h.pcr @16:02:29.52 Building PCR Slug from extSrc with MM3 mix, PP5 primer @16:02:29.56 Priming with carrier @16:02:29.57 ATV.dial ARV @16:02:31.07 ARV.dial :carrier @16:02:31.42 AS.reconfigure ASnorm @16:02:31.48 AS.seek 0.499ml @16:02:57.06 AFV.select :carrierSolution @16:02:57.18 delay 2 seconds @16:02:59.20 AS.reconfigure ASconfig @16:02:59.27 AS.seek 1ml @16:03:28.35 delay 1 second @16:03:29.37 AFV.select :holdingCoil @16:03:29.48 delay 1 second @16:03:30.50 ATV.dial :waste @16:03:32.18 AS.reconfigure ASnorm @16:03:32.24 AS.seek :empty @16:04:22.99 AFV.select :carrierSolution @16:04:23.11 delay 2 seconds @16:04:25.13 AS.reconfigure ASconfig @16:04:25.19 AS.seek 0.5ml @16:04:54.31 delay 1 second @16:04:55.33 AFV.select :holdingCoil @16:04:55.44 Building Slug @16:04:55.45 ATV.dial :ARV @16:04:57.21 delay 2 seconds @16:04:59.23 ARV.dial :air1 @16:04:59.74 delay 2 seconds @16:05:01.81 AS.reconfigure ASslow2 @16:05:01.87 AS.seek 0.506ml @16:05:08.16 delay 2 seconds @16:05:10.18 ARV.dial :MM3 @16:05:11.24 delay 2 seconds @16:05:13.27 Adding MM3 @16:05:13.36 AS.seek 0.524ml @16:05:31.78 delay 25 seconds @16:05:56.80 ARV.dial :PP5 @16:05:57.17 Adding PP5 @16:05:57.19 delay 2 seconds @16:05:59.27 AS.seek 0.53ml @16:06:05.74 delay 10 seconds @16:06:15.77 ARV.dial :air3 @16:06:16.16 adding ARV air @16:06:16.18 delay 2 seconds @16:06:18.26 AS.seek 0.536ml @16:06:24.52 delay 5 seconds @16:06:29.54 ARV.dial :carrier @16:06:30.80 delay 7 seconds @16:06:37.89 AS.seek 0.594ml @16:07:36.44 delay 5 seconds @16:07:41.46 ATV.dial :extSrc @16:07:42.02 delay 2 seconds @16:07:44.10 AS.seek 0.6ml @16:07:50.49 delay 4 seconds @16:07:54.51 ATV.dial ARV @16:07:55.06 delay 2 seconds @16:07:57.08 ARV.dial :carrier @16:07:57.10 delay 2 seconds @16:07:59.18 AS.seek 0.65ml @16:08:49.69 delay 4 seconds @16:08:53.71 ATV.dial PCR @16:08:54.49 ASV.dial :waste1 @16:08:54.91 ARV.dial :air1 @16:08:55.39 Positioning Slug by 0.324ml @16:08:55.48 AS.seek 0.326ml @16:14:21.83 delay 5 seconds @16:14:26.85 ATV.dial ARV @16:14:27.64 ASV.dial :PCRlock @16:14:28.05 delay 5 seconds @16:14:33.08 Running OM Profile60C350 thermocycling @16:14:33.09 PCR.program Profile60C350 @16:14:33.22 Profile60C350 should complete in < 2:06:05 @16:14:33.23 PCR.run 2:42:36.25 @17:52:15.42 PCR.off @17:52:17.26 PCR for OM completed -- closed /var/log/esp/OM20mar1816h.pcr @17:52:17.34 Cleaning PCR @17:52:17.35 AFV.select :carrierSolution @17:52:17.47 delay 2 seconds @17:52:19.49 AS.reconfigure ASconfig @17:52:19.55 AS.seek 0.5ml @17:52:29.85 delay 1 second @17:52:30.87 AFV.select :holdingCoil @17:52:30.97 ASV.dial :waste1 @17:52:31.36 ATV.dial :air3 @17:52:32.66 AS.reconfigure ASslow1 @17:52:32.72 AS.seek 0.51ml @17:52:35.03 delay 2 seconds @17:52:37.05 ATV.dial :bleach @17:52:37.67 AS.seek 0.81ml @17:53:38.27 delay 3 seconds @17:53:41.29 ATV.dial :air3 @17:53:41.85 delay 3 seconds @17:53:44.93 AS.seek 0.835ml @17:53:50.31 delay 3 seconds @17:53:53.33 ATV.dial :bleach @17:53:53.95 AS.seek 0.82ml @17:53:57.25 delay 3 seconds @17:54:00.27 ARV.dial :waste @17:54:00.88 delay 3 seconds @17:54:03.90 ATV.dial :ARV @17:54:04.80 AS.reconfigure ASconfig @17:54:04.86 AS.seek 0.47ml @17:54:25.27 AFV.select :carrierSolution @17:54:25.38 delay 2 seconds @17:54:27.40 AS.seek 1ml @17:54:58.13 delay 1 second @17:54:59.15 AFV.select :holdingCoil @17:54:59.26 delay 1 second @17:55:00.28 AS.reconfigure ASfast @17:55:00.33 AS.seek 0ml @17:55:27.69 AFV.select :carrierSolution @17:55:27.81 delay 2 seconds @17:55:29.83 AS.reconfigure ASconfig @17:55:29.88 AS.seek 1ml @17:56:27.69 delay 1 second @17:56:28.71 AFV.select :holdingCoil @17:56:28.82 delay 1 second @17:56:29.84 AS.reconfigure ASfast @17:56:29.89 AS.seek 0ml @17:56:57.25 AFV.select :carrierSolution @17:56:57.37 delay 2 seconds @17:56:59.39 AS.reconfigure ASconfig @17:56:59.44 AS.seek 1ml @17:57:57.19 delay 1 second @17:57:58.21 AFV.select :holdingCoil @17:57:58.33 delay 1 second @17:57:59.35 AS.reconfigure ASfast @17:57:59.40 AS.seek 0ml @17:58:26.74 ARV.dial between carrier and air1 @17:58:27.24 ATV.dial :air3 @17:58:28.55 AS.reconfigure ASslow1 @17:58:28.61 AS.seek 0.01ml @17:58:30.93 delay 2 seconds @17:58:32.95 ATV.dial :bleach @17:58:33.58 AS.seek 0.81ml @18:01:14.74 delay 3 seconds @18:01:17.76 ATV.dial :air3 @18:01:18.33 delay 3 seconds @18:01:21.40 AS.seek 0.835ml @18:01:26.71 delay 3 seconds @18:01:29.73 ATV.dial :bleach @18:01:30.38 AS.seek 0.82ml @18:01:33.69 delay 3 seconds @18:01:36.71 ATV.dial PCR @18:01:37.12 AS.reconfigure ASconfig @18:01:37.17 AS.seek :empty @18:02:24.63 PCR.program :bleachProfile @18:02:24.69 PCR program should complete in < 2:45 @18:02:24.70 PCR.run 10:00 @18:05:20.82 PCR.off @18:05:20.86 AFV.select :carrierSolution @18:05:20.98 delay 2 seconds @18:05:23.00 AS.seek 1ml @18:06:20.83 delay 1 second @18:06:21.85 AFV.select :holdingCoil @18:06:21.96 delay 1 second @18:06:22.98 AS.reconfigure ASfast @18:06:23.04 AS.seek 0ml @18:06:50.40 delay 3 seconds @18:06:53.42 AFV.select :carrierSolution @18:06:53.53 delay 2 seconds @18:06:55.55 AS.reconfigure ASconfig @18:06:55.61 AS.seek 1ml @18:07:53.38 delay 1 second @18:07:54.40 AFV.select :holdingCoil @18:07:54.51 delay 1 second @18:07:55.53 AS.reconfigure ASfast @18:07:55.59 AS.seek 0ml @18:08:22.94 delay 3 seconds @18:08:25.96 AFV.select :carrierSolution @18:08:26.07 delay 2 seconds @18:08:28.09 AS.reconfigure ASconfig @18:08:28.15 AS.seek 1ml @18:09:25.93 delay 1 second @18:09:26.95 AFV.select :holdingCoil @18:09:27.06 delay 1 second @18:09:28.08 AS.reconfigure ASfast @18:09:28.14 AS.seek 0ml @18:09:55.50 delay 3 seconds @18:09:58.52 AFV.select :carrierSolution @18:09:58.63 delay 2 seconds @18:10:00.65 AS.reconfigure ASconfig @18:10:00.71 AS.seek 1ml @18:10:58.50 delay 1 second @18:10:59.52 AFV.select :holdingCoil @18:10:59.63 delay 1 second @18:11:00.65 AS.reconfigure ASfast @18:11:00.70 AS.seek 0ml @18:11:28.07 delay 3 seconds @18:11:31.09 AFV.select :carrierSolution @18:11:31.20 delay 2 seconds @18:11:33.22 AS.reconfigure ASconfig @18:11:33.29 AS.seek 1ml @18:12:31.04 delay 1 second @18:12:32.06 AFV.select :holdingCoil @18:12:32.17 delay 1 second @18:12:33.19 AS.reconfigure ASnorm @18:12:33.24 AS.seek 0ml @18:13:23.98
AFV.select :carrierSolution @18:13:24.10 delay 2 seconds @18:13:26.12 AS.reconfigure ASconfig @18:13:26.17 AS.seek 0.5ml @18:13:55.21 delay 1 second @18:13:56.23 AFV.select :holdingCoil @18:13:56.35 ATV.dial :air1 @18:13:57.85 AS.reconfigure ASslow2 @18:13:57.91 AS.seek 0.506ml @18:14:04.32 ATV.dial :extSrc @18:14:06.03 AS.seek 0.512ml @18:14:12.41 delay 5 seconds @18:14:17.43 ATV.dial :air1 @18:14:19.17 AS.reconfigure ASslow1 @18:14:19.22 AS.seek 0.542ml @18:14:25.58 ATV.dial :waste @18:14:26.16 AS.reconfigure ASconfig @18:14:26.21 AS.seek :empty @18:14:57.88 Setting up PCR for OM -- writing /var/log/esp/OM20mar1818h.pcr @18:14:57.91 Building PCR Slug from extSrc with MM3 mix, PP5 primer @18:14:57.93 Priming with carrier @18:14:57.95 ATV.dial ARV @18:14:59.63 ARV.dial :carrier @18:14:59.99 AS.reconfigure ASnorm @18:15:00.05 AS.seek 0.499ml @18:15:25.68 AFV.select :carrierSolution @18:15:25.80 delay 2 seconds @18:15:27.82 AS.reconfigure ASconfig @18:15:27.89 AS.seek 1ml @18:15:56.96 delay 1 second @18:15:57.98 AFV.select :holdingCoil @18:15:58.09 delay 1 second @18:15:59.10 ATV.dial :waste @18:16:00.76 AS.reconfigure ASnorm @18:16:00.82 AS.seek :empty @18:16:51.55 AFV.select :carrierSolution @18:16:51.67 delay 2 seconds @18:16:53.69 AS.reconfigure ASconfig @18:16:53.75 AS.seek 0.5ml @18:17:22.83 delay 1 second @18:17:23.85 AFV.select :holdingCoil @18:17:23.96 Building Slug @18:17:23.97 ATV.dial :ARV @18:17:25.72 delay 2 seconds @18:17:27.74 ARV.dial :air1 @18:17:28.15 delay 2 seconds @18:17:30.22 AS.reconfigure ASslow2 @18:17:30.28 AS.seek 0.506ml @18:17:36.75 delay 2 seconds @18:17:38.77 ARV.dial :MM3 @18:17:39.82 delay 2 seconds @18:17:41.85 Adding MM3 @18:17:41.94 AS.seek 0.524ml @18:18:00.30 delay 25 seconds @18:18:25.32 ARV.dial :PP5 @18:18:25.72 Adding PP5 @18:18:25.74 delay 2 seconds @18:18:27.82 AS.seek 0.53ml @18:18:34.00 delay 10 seconds @18:18:44.03 ARV.dial :air3 @18:18:44.43 adding ARV air @18:18:44.45 delay 2 seconds @18:18:46.53 AS.seek 0.536ml @18:18:52.86 delay 5 seconds @18:18:57.88 ARV.dial :carrier @18:18:59.15 delay 7 seconds @18:19:06.24 AS.seek 0.594ml @18:20:04.97 delay 5 seconds @18:20:09.99 ATV.dial :extSrc @18:20:10.66 delay 2 seconds @18:20:12.74 AS.seek 0.6ml @18:20:19.16 delay 4 seconds @18:20:23.18 ATV.dial ARV @18:20:23.83 delay 2 seconds @18:20:25.85 ARV.dial :carrier @18:20:25.87 delay 2 seconds @18:20:27.95 AS.seek 0.65ml @18:21:18.66 delay 4 seconds @18:21:22.68 ATV.dial PCR @18:21:23.46 ASV.dial :waste1 @18:21:23.47 ARV.dial :air1 @18:21:23.89 Positioning Slug by 0.324ml @18:21:23.99 AS.seek 0.326ml @18:26:49.94 delay 5 seconds @18:26:55.37 ATV.dial ARV @18:26:56.16 ASV.dial :PCRlock @18:26:56.57 delay 5 seconds @18:27:01.60 Running OM Profile60C350 thermocycling @18:27:01.61 PCR.program Profile60C350 @18:27:01.74 Profile60C350 should complete in < 2:06:05 @18:27:01.75 PCR.run 2:42:36.25 @20:04:28.14 PCR.off @20:04:29.98 PCR for OM completed -- closed /var/log/esp/OM20mar1818h.pcr @20:04:30.06 Cleaning PCR @20:04:30.07 AFV.select :carrierSolution @20:04:30.20 delay 2 seconds @20:04:32.22 AS.reconfigure ASconfig @20:04:32.27 AS.seek 0.5ml @20:04:42.60 delay 1 second @20:04:43.62 AFV.select :holdingCoil @20:04:43.72 ASV.dial :waste1 @20:04:44.12 ATV.dial :air3 @20:04:45.41 AS.reconfigure ASslow1 @20:04:45.47 AS.seek 0.51ml @20:04:47.77 delay 2 seconds @20:04:49.79 ATV.dial :bleach @20:04:50.41 AS.seek 0.81ml @20:05:50.99 delay 3 seconds @20:05:54.01 ATV.dial :air3 @20:05:54.57 delay 3 seconds @20:05:57.65 AS.seek 0.835ml @20:06:02.97 delay 3 seconds @20:06:05.99 ATV.dial :bleach @20:06:06.61 AS.seek 0.82ml @20:06:09.93 delay 3 seconds @20:06:12.95 ARV.dial :waste @20:06:13.55 delay 3 seconds @20:06:16.57 ATV.dial :ARV @20:06:17.47 AS.reconfigure ASconfig @20:06:17.53 AS.seek 0.47ml @20:06:37.94 AFV.select :carrierSolution @20:06:38.06 delay 2 seconds @20:06:40.08 AS.seek 1ml @20:07:10.80 delay 1 second @20:07:11.82 AFV.select :holdingCoil @20:07:11.93 delay 1 second @20:07:12.95 AS.reconfigure ASfast @20:07:13.01 AS.seek 0ml @20:07:40.37 AFV.select :carrierSolution @20:07:40.49 delay 2 seconds @20:07:42.51 AS.reconfigure ASconfig @20:07:42.57 AS.seek 1ml @20:08:40.32 delay 1 second @20:08:41.34 AFV.select :holdingCoil @20:08:41.45 delay 1 second @20:08:42.47 AS.reconfigure ASfast @20:08:42.53 AS.seek 0ml @20:09:09.87 AFV.select :carrierSolution @20:09:09.99 delay 2 seconds @20:09:12.01 AS.reconfigure ASconfig @20:09:12.07 AS.seek 1ml @20:10:09.85 delay 1 second @20:10:10.87 AFV.select :holdingCoil @20:10:10.98 delay 1 second @20:10:12.00 AS.reconfigure ASfast @20:10:12.05 AS.seek 0ml @20:10:39.41 ARV.dial between carrier and air1 @20:10:39.91 ATV.dial :air3 @20:10:41.22 AS.reconfigure ASslow1 @20:10:41.28 AS.seek 0.01ml @20:10:43.66 delay 2 seconds @20:10:45.68 ATV.dial :bleach @20:10:46.32 AS.seek 0.81ml @20:13:27.51 delay 3 seconds @20:13:30.53 ATV.dial :air3 @20:13:31.10 delay 3 seconds @20:13:34.17 AS.seek 0.835ml @20:13:39.52 delay 3 seconds @20:13:42.54 ATV.dial :bleach @20:13:43.18 AS.seek 0.82ml @20:13:46.49 delay 3 seconds @20:13:49.51 ATV.dial PCR @20:13:49.92 AS.reconfigure ASconfig @20:13:49.97 AS.seek :empty @20:14:37.43 PCR.program :bleachProfile @20:14:37.49 PCR program should complete in < 2:45 @20:14:37.50 PCR.run 10:00 @20:17:33.65 PCR.off @20:17:33.69 AFV.select :carrierSolution @20:17:33.81 delay 2 seconds @20:17:35.83 AS.seek 1ml @20:18:33.66 delay 1 second @20:18:34.68 AFV.select :holdingCoil @20:18:34.79 delay 1 second @20:18:35.81 AS.reconfigure ASfast @20:18:35.87 AS.seek 0ml @20:19:03.23 delay 3 seconds @20:19:06.25 AFV.select :carrierSolution @20:19:06.36 delay 2 seconds @20:19:08.38 AS.reconfigure ASconfig @20:19:08.44 AS.seek 1ml @20:20:06.23 delay 1 second @20:20:07.25 AFV.select :holdingCoil @20:20:07.36 delay 1 second @20:20:08.38 AS.reconfigure ASfast @20:20:08.43 AS.seek 0ml @20:20:35.79 delay 3 seconds @20:20:38.81 AFV.select :carrierSolution @20:20:38.92 delay 2 seconds @20:20:40.94 AS.reconfigure ASconfig @20:20:41.01 AS.seek 1ml @20:21:38.79 delay 1 second @20:21:39.81 AFV.select :holdingCoil @20:21:39.92 delay 1 second @20:21:40.94 AS.reconfigure ASfast @20:21:41.00 AS.seek 0ml @20:22:08.35 delay 3 seconds @20:22:11.37 AFV.select :carrierSolution @20:22:11.48 delay 2 seconds @20:22:13.50 AS.reconfigure ASconfig @20:22:13.57 AS.seek 1ml @20:23:11.32 delay 1 second @20:23:12.34 AFV.select :holdingCoil @20:23:12.45 delay 1 second @20:23:13.47 AS.reconfigure ASfast @20:23:13.53 AS.seek 0ml @20:23:40.88 delay 3 seconds @20:23:43.90 AFV.select :carrierSolution @20:23:44.01 delay 2 seconds @20:23:46.03 AS.reconfigure ASconfig @20:23:46.10 AS.seek 1ml @20:24:43.91 delay 1 second @20:24:44.93 AFV.select :holdingCoil @20:24:45.04 delay 1 second @20:24:46.06 AS.reconfigure ASnorm @20:24:46.11 AS.seek 0ml @20:25:36.82
AFV.select :carrierSolution @20:25:36.94 delay 2 seconds @20:25:38.96 AS.reconfigure ASconfig @20:25:39.01 AS.seek 0.5ml @20:26:08.04 delay 1 second @20:26:09.06 AFV.select :holdingCoil @20:26:09.17 ATV.dial :air1 @20:26:10.66 AS.reconfigure ASslow2 @20:26:10.72 AS.seek 0.51ml @20:26:21.23 delay 5 seconds @20:26:26.25 ATV.dial :extSrc @20:26:27.97 AS.seek 0.53ml @20:26:48.32 delay 10 seconds @20:26:58.35 ATV.dial :air1 @20:27:00.05 AS.reconfigure ASslow1 @20:27:00.11 AS.seek 0.56ml @20:27:06.40 ATV.dial :waste @20:27:06.96 AS.reconfigure ASconfig @20:27:07.01 AS.seek :empty @20:27:39.71 Setting up PCR for CG -- writing /var/log/esp/CG20mar1820h.pcr @20:27:39.73 Building PCR Slug from extSrc with MM1 mix, PP2 primer @20:27:39.76 Priming with carrier @20:27:39.78 ATV.dial ARV @20:27:41.29 ARV.dial :carrier @20:27:41.65 AS.reconfigure ASnorm @20:27:41.72 AS.seek 0.499ml @20:28:07.29 AFV.select :carrierSolution @20:28:07.41 delay 2 seconds @20:28:09.43 AS.reconfigure ASconfig @20:28:09.49 AS.seek 1ml @20:28:38.57 delay 1 second @20:28:39.59 AFV.select :holdingCoil @20:28:39.70 delay 1 second @20:28:40.71 ATV.dial :waste @20:28:42.39 AS.reconfigure ASnorm @20:28:42.45 AS.seek :empty @20:29:33.20 AFV.select :carrierSolution @20:29:33.32 delay 2 seconds @20:29:35.34 AS.reconfigure ASconfig @20:29:35.40 AS.seek 0.5ml @20:30:04.51 delay 1 second @20:30:05.53 AFV.select :holdingCoil @20:30:05.64 Building Slug @20:30:05.65 ATV.dial :ARV @20:30:07.30 delay 2 seconds @20:30:09.32 ARV.dial :air1 @20:30:09.73 delay 2 seconds @20:30:11.80 AS.reconfigure ASslow2 @20:30:11.86 AS.seek 0.506ml @20:30:18.12 delay 2 seconds @20:30:20.14 ARV.dial :MM1 @20:30:20.76 delay 2 seconds @20:30:22.79 Adding MM1 @20:30:22.86 AS.seek 0.524ml @20:30:41.20 delay 25 seconds @20:31:06.22 ARV.dial :PP2 @20:31:06.62 Adding PP2 @20:31:06.64 delay 2 seconds @20:31:08.72 AS.seek 0.53ml @20:31:15.34 delay 10 seconds @20:31:25.37 ARV.dial :air2 @20:31:25.76 adding ARV air @20:31:25.78 delay 2 seconds @20:31:27.86 AS.seek 0.536ml @20:31:34.04 delay 5 seconds @20:31:39.06 ARV.dial :carrier @20:31:40.34 delay 7 seconds @20:31:47.43 AS.seek 0.594ml @20:32:45.98 delay 5 seconds @20:32:51.00 ATV.dial :extSrc @20:32:51.59 delay 2 seconds @20:32:53.67 AS.seek 0.6ml @20:33:00.24 delay 4 seconds @20:33:04.26 ATV.dial ARV @20:33:04.82 delay 2 seconds @20:33:06.84 ARV.dial :carrier @20:33:06.86 delay 2 seconds @20:33:08.94 AS.seek 0.65ml @20:33:59.46 delay 4 seconds @20:34:03.48 ATV.dial PCR @20:34:04.26 ASV.dial :waste1 @20:34:04.28 ARV.dial :air1 @20:34:04.70 Positioning Slug by 0.324ml @20:34:04.80 AS.seek 0.326ml @20:39:31.09 delay 5 seconds @20:39:36.11 ATV.dial ARV @20:39:36.90 ASV.dial :PCRlock @20:39:37.30 delay 5 seconds @20:39:42.33 Running CG Profile60C300 thermocycling @20:39:42.34 PCR.program Profile60C300 @20:39:42.45 Profile60C300 should complete in < 1:40:53 @20:39:42.46 PCR.run 2:11:06.25 @22:00:55.23 PCR.off @22:00:55.81 PCR for CG completed -- closed /var/log/esp/CG20mar1820h.pcr @22:00:55.88 Cleaning PCR @22:00:55.89 AFV.select :carrierSolution @22:00:56.01 delay 2 seconds @22:00:58.03 AS.reconfigure ASconfig @22:00:58.08 AS.seek 0.5ml @22:01:08.43 delay 1 second @22:01:09.45 AFV.select :holdingCoil @22:01:09.55 ASV.dial :waste1 @22:01:09.95 ATV.dial :air3 @22:01:11.26 AS.reconfigure ASslow1 @22:01:11.32 AS.seek 0.51ml @22:01:13.62 delay 2 seconds @22:01:15.64 ATV.dial :bleach @22:01:16.27 AS.seek 0.81ml @22:02:16.85 delay 3 seconds @22:02:19.87 ATV.dial :air3 @22:02:20.45 delay 3 seconds @22:02:23.53 AS.seek 0.835ml @22:02:28.81 delay 3 seconds @22:02:31.83 ATV.dial :bleach @22:02:32.46 AS.seek 0.82ml @22:02:35.76 delay 3 seconds @22:02:38.78 ARV.dial :waste @22:02:39.40 delay 3 seconds @22:02:42.42 ATV.dial :ARV @22:02:43.32 AS.reconfigure ASconfig @22:02:43.38 AS.seek 0.47ml @22:03:03.81 AFV.select :carrierSolution @22:03:03.92 delay 2 seconds @22:03:05.94 AS.seek 1ml @22:03:36.68 delay 1 second @22:03:37.70 AFV.select :holdingCoil @22:03:37.81 delay 1 second @22:03:38.83 AS.reconfigure ASfast @22:03:38.88 AS.seek 0ml @22:04:06.24 AFV.select :carrierSolution @22:04:06.77 delay 2 seconds @22:04:08.79 AS.reconfigure ASconfig @22:04:08.85 AS.seek 1ml @22:05:06.59 delay 1 second @22:05:07.61 AFV.select :holdingCoil @22:05:07.72 delay 1 second @22:05:08.74 AS.reconfigure ASfast @22:05:08.80 AS.seek 0ml @22:05:36.15 AFV.select :carrierSolution @22:05:36.27 delay 2 seconds @22:05:38.29 AS.reconfigure ASconfig @22:05:38.35 AS.seek 1ml @22:06:36.15 delay 1 second @22:06:37.17 AFV.select :holdingCoil @22:06:37.28 delay 1 second @22:06:38.30 AS.reconfigure ASfast @22:06:38.36 AS.seek 0ml @22:07:05.70 ARV.dial between carrier and air1 @22:07:06.21 ATV.dial :air3 @22:07:07.52 AS.reconfigure ASslow1 @22:07:07.58 AS.seek 0.01ml @22:07:09.92 delay 2 seconds @22:07:11.94 ATV.dial :bleach @22:07:12.59 AS.seek 0.81ml @22:09:53.74 delay 3 seconds @22:09:56.76 ATV.dial :air3 @22:09:57.34 delay 3 seconds @22:10:00.42 AS.seek 0.835ml @22:10:05.73 delay 3 seconds @22:10:08.75 ATV.dial :bleach @22:10:09.38 AS.seek 0.82ml @22:10:12.70 delay 3 seconds @22:10:15.72 ATV.dial PCR @22:10:16.13 AS.reconfigure ASconfig @22:10:16.18 AS.seek :empty @22:11:03.65 PCR.program :bleachProfile @22:11:03.71 PCR program should complete in < 2:45 @22:11:03.72 PCR.run 10:00 @22:13:59.49 PCR.off @22:13:59.53 AFV.select :carrierSolution @22:13:59.65 delay 2 seconds @22:14:01.67 AS.seek 1ml @22:14:59.51 delay 1 second @22:15:00.53 AFV.select :holdingCoil @22:15:00.64 delay 1 second @22:15:01.66 AS.reconfigure ASfast @22:15:01.72 AS.seek 0ml @22:15:29.04 delay 3 seconds @22:15:32.06 AFV.select :carrierSolution @22:15:32.17 delay 2 seconds @22:15:34.19 AS.reconfigure ASconfig @22:15:34.26 AS.seek 1ml @22:16:32.06 delay 1 second @22:16:33.08 AFV.select :holdingCoil @22:16:33.19 delay 1 second @22:16:34.21 AS.reconfigure ASfast @22:16:34.26 AS.seek 0ml @22:17:01.60 delay 3 seconds @22:17:04.62 AFV.select :carrierSolution @22:17:04.73 delay 2 seconds @22:17:06.75 AS.reconfigure ASconfig @22:17:06.81 AS.seek 1ml @22:18:04.59 delay 1 second @22:18:05.61 AFV.select :holdingCoil @22:18:05.72 delay 1 second @22:18:06.74 AS.reconfigure ASfast @22:18:06.79 AS.seek 0ml @22:18:34.15 delay 3 seconds @22:18:37.17 AFV.select :carrierSolution @22:18:37.28 delay 2 seconds @22:18:39.30 AS.reconfigure ASconfig @22:18:39.36 AS.seek 1ml @22:19:37.10 delay 1 second @22:19:38.12 AFV.select :holdingCoil @22:19:38.23 delay 1 second @22:19:39.25 AS.reconfigure ASfast @22:19:39.30 AS.seek 0ml @22:20:06.65 delay 3 seconds @22:20:09.67 AFV.select :carrierSolution @22:20:09.78 delay 2 seconds @22:20:11.80 AS.reconfigure ASconfig @22:20:11.87 AS.seek 1ml @22:21:09.64 delay 1 second @22:21:10.66 AFV.select :holdingCoil @22:21:10.77 delay 1 second @22:21:11.79 AS.reconfigure ASnorm @22:21:11.84 AS.seek 0ml @22:22:02.59
AFV.select :carrierSolution @22:22:02.71 delay 2 seconds @22:22:04.73 AS.reconfigure ASconfig @22:22:04.78 AS.seek 0.5ml @22:22:33.81 delay 1 second @22:22:34.83 AFV.select :holdingCoil @22:22:34.94 ATV.dial :air1 @22:22:36.43 AS.reconfigure ASslow2 @22:22:36.49 AS.seek 0.506ml @22:22:42.92 ATV.dial :extSrc @22:22:44.63 AS.seek 0.512ml @22:22:50.93 delay 5 seconds @22:22:55.95 ATV.dial :air1 @22:22:57.66 AS.reconfigure ASslow1 @22:22:57.71 AS.seek 0.542ml @22:23:04.01 ATV.dial :waste @22:23:05.05 AS.reconfigure ASconfig @22:23:05.10 AS.seek :empty @22:23:36.73 Setting up PCR for CG -- writing /var/log/esp/CG20mar1822h.pcr @22:23:36.76 Building PCR Slug from extSrc with MM1 mix, PP2 primer @22:23:36.79 Priming with carrier @22:23:36.80 ATV.dial ARV @22:23:38.32 ARV.dial :carrier @22:23:38.66 AS.reconfigure ASnorm @22:23:38.73 AS.seek 0.499ml @22:24:04.34 AFV.select :carrierSolution @22:24:04.47 delay 2 seconds @22:24:06.49 AS.reconfigure ASconfig @22:24:06.55 AS.seek 1ml @22:24:35.64 delay 1 second @22:24:36.66 AFV.select :holdingCoil @22:24:36.77 delay 1 second @22:24:37.78 ATV.dial :waste @22:24:39.47 AS.reconfigure ASnorm @22:24:39.53 AS.seek :empty @22:25:30.25 AFV.select :carrierSolution @22:25:30.37 delay 2 seconds @22:25:32.39 AS.reconfigure ASconfig @22:25:32.46 AS.seek 0.5ml @22:26:01.59 delay 1 second @22:26:02.61 AFV.select :holdingCoil @22:26:02.72 Building Slug @22:26:02.73 ATV.dial :ARV @22:26:04.39 delay 2 seconds @22:26:06.41 ARV.dial :air1 @22:26:06.83 delay 2 seconds @22:26:08.90 AS.reconfigure ASslow2 @22:26:08.97 AS.seek 0.506ml @22:26:15.50 delay 2 seconds @22:26:17.52 ARV.dial :MM1 @22:26:18.10 delay 2 seconds @22:26:20.13 Adding MM1 @22:26:20.20 AS.seek 0.524ml @22:26:38.78 delay 25 seconds @22:27:03.80 ARV.dial :PP2 @22:27:04.20 Adding PP2 @22:27:04.22 delay 2 seconds @22:27:06.30 AS.seek 0.53ml @22:27:12.81 delay 10 seconds @22:27:22.83 ARV.dial :air2 @22:27:23.23 adding ARV air @22:27:23.25 delay 2 seconds @22:27:25.34 AS.seek 0.536ml @22:27:31.73 delay 5 seconds @22:27:36.75 ARV.dial :carrier @22:27:38.03 delay 7 seconds @22:27:45.12 AS.seek 0.594ml @22:28:43.79 delay 5 seconds @22:28:48.81 ATV.dial :extSrc @22:28:49.50 delay 2 seconds @22:28:51.58 AS.seek 0.6ml @22:28:57.85 delay 4 seconds @22:29:01.87 ATV.dial ARV @22:29:02.42 delay 2 seconds @22:29:04.44 ARV.dial :carrier @22:29:04.46 delay 2 seconds @22:29:06.54 AS.seek 0.65ml @22:29:57.07 delay 4 seconds @22:30:01.09 ATV.dial PCR @22:30:01.87 ASV.dial :waste1 @22:30:01.88 ARV.dial :air1 @22:30:02.41 Positioning Slug by 0.324ml @22:30:02.50 AS.seek 0.326ml @22:35:28.65 delay 5 seconds @22:35:33.67 ATV.dial ARV @22:35:34.46 ASV.dial :PCRlock @22:35:34.87 delay 5 seconds @22:35:39.90 Running CG Profile60C300 thermocycling @22:35:39.91 PCR.program Profile60C300 @22:35:40.03 Profile60C300 should complete in < 1:40:53 @22:35:40.04 PCR.run 2:11:06.25 @23:56:55.02 PCR.off @23:56:55.59 PCR for CG completed -- closed /var/log/esp/CG20mar1822h.pcr @23:56:55.67 Cleaning PCR @23:56:55.68 AFV.select :carrierSolution @23:56:55.80 delay 2 seconds @23:56:57.82 AS.reconfigure ASconfig @23:56:57.88 AS.seek 0.5ml @23:57:08.20 delay 1 second @23:57:09.22 AFV.select :holdingCoil @23:57:09.32 ASV.dial :waste1 @23:57:09.72 ATV.dial :air3 @23:57:11.01 AS.reconfigure ASslow1 @23:57:11.07 AS.seek 0.51ml @23:57:13.35 delay 2 seconds @23:57:15.37 ATV.dial :bleach @23:57:16.00 AS.seek 0.81ml @23:58:16.59 delay 3 seconds @23:58:19.61 ATV.dial :air3 @23:58:20.17 delay 3 seconds @23:58:23.24 AS.seek 0.835ml @23:58:28.52 delay 3 seconds @23:58:31.54 ATV.dial :bleach @23:58:32.15 AS.seek 0.82ml @23:58:35.47 delay 3 seconds @23:58:38.49 ARV.dial :waste @23:58:39.11 delay 3 seconds @23:58:42.13 ATV.dial :ARV @23:58:43.03 AS.reconfigure ASconfig @23:58:43.09 AS.seek 0.47ml @23:59:03.50 AFV.select :carrierSolution @23:59:03.62 delay 2 seconds @23:59:05.64 AS.seek 1ml @23:59:36.39 delay 1 second @23:59:37.41 AFV.select :holdingCoil @23:59:37.52 delay 1 second @23:59:38.54 AS.reconfigure ASfast @23:59:38.60 AS.seek 0ml @00:00:05.92CET19-Mar-20 AFV.select :carrierSolution @00:00:06.04 delay 2 seconds @00:00:08.06 AS.reconfigure ASconfig @00:00:08.12 AS.seek 1ml @00:01:05.87 delay 1 second @00:01:06.89 AFV.select :holdingCoil @00:01:07.00 delay 1 second @00:01:08.02 AS.reconfigure ASfast @00:01:08.08 AS.seek 0ml @00:01:35.42 AFV.select :carrierSolution @00:01:35.54 delay 2 seconds @00:01:37.56 AS.reconfigure ASconfig @00:01:37.62 AS.seek 1ml @00:02:35.36 delay 1 second @00:02:36.38 AFV.select :holdingCoil @00:02:36.49 delay 1 second @00:02:37.51 AS.reconfigure ASfast @00:02:37.58 AS.seek 0ml @00:03:04.94 ARV.dial between carrier and air1 @00:03:05.45 ATV.dial :air3 @00:03:06.75 AS.reconfigure ASslow1 @00:03:06.81 AS.seek 0.01ml @00:03:09.12 delay 2 seconds @00:03:11.14 ATV.dial :bleach @00:03:11.76 AS.seek 0.81ml @00:05:52.92 delay 3 seconds @00:05:55.94 ATV.dial :air3 @00:05:56.51 delay 3 seconds @00:05:59.59 AS.seek 0.835ml @00:06:04.90 delay 3 seconds @00:06:07.92 ATV.dial :bleach @00:06:08.56 AS.seek 0.82ml @00:06:11.89 delay 3 seconds @00:06:14.91 ATV.dial PCR @00:06:15.32 AS.reconfigure ASconfig @00:06:15.37 AS.seek :empty @00:07:02.84 PCR.program :bleachProfile @00:07:02.90 PCR program should complete in < 2:45 @00:07:02.91 PCR.run 10:00 @00:09:59.05 PCR.off @00:09:59.09 AFV.select :carrierSolution @00:09:59.21 delay 2 seconds @00:10:01.23 AS.seek 1ml @00:10:59.08 delay 1 second @00:11:00.10 AFV.select :holdingCoil @00:11:00.21 delay 1 second @00:11:01.23 AS.reconfigure ASfast @00:11:01.29 AS.seek 0ml @00:11:28.64 delay 3 seconds @00:11:31.66 AFV.select :carrierSolution @00:11:31.77 delay 2 seconds @00:11:33.79 AS.reconfigure ASconfig @00:11:33.86 AS.seek 1ml @00:12:31.59 delay 1 second @00:12:32.61 AFV.select :holdingCoil @00:12:32.72 delay 1 second @00:12:33.74 AS.reconfigure ASfast @00:12:33.80 AS.seek 0ml @00:13:01.15 delay 3 seconds @00:13:04.17 AFV.select :carrierSolution @00:13:04.28 delay 2 seconds @00:13:06.30 AS.reconfigure ASconfig @00:13:06.37 AS.seek 1ml @00:14:04.14 delay 1 second @00:14:05.16 AFV.select :holdingCoil @00:14:05.27 delay 1 second @00:14:06.29 AS.reconfigure ASfast @00:14:06.35 AS.seek 0ml @00:14:33.70 delay 3 seconds @00:14:36.72 AFV.select :carrierSolution @00:14:36.83 delay 2 seconds @00:14:38.85 AS.reconfigure ASconfig @00:14:38.91 AS.seek 1ml @00:15:36.67 delay 1 second @00:15:37.69 AFV.select :holdingCoil @00:15:37.80 delay 1 second @00:15:38.82 AS.reconfigure ASfast @00:15:38.89 AS.seek 0ml @00:16:06.25 delay 3 seconds @00:16:09.27 AFV.select :carrierSolution @00:16:09.38 delay 2 seconds @00:16:11.40 AS.reconfigure ASconfig @00:16:11.46 AS.seek 1ml @00:17:09.20 delay 1 second @00:17:10.22 AFV.select :holdingCoil @00:17:10.33 delay 1 second @00:17:11.35 AS.reconfigure ASnorm @00:17:11.41 AS.seek 0ml @00:18:02.13
AFV.select :carrierSolution @00:18:02.24 delay 2 seconds @00:18:04.26 AS.reconfigure ASconfig @00:18:04.31 AS.seek 0.5ml @00:18:33.39 delay 1 second @00:18:34.41 AFV.select :holdingCoil @00:18:34.52 ATV.dial :air1 @00:18:36.01 AS.reconfigure ASslow1 @00:18:36.07 AS.seek 0.52ml @00:18:40.39 delay 5 seconds @00:18:45.41 ATV.dial :extSrc @00:18:47.14 AS.reconfigure ASslow2 @00:18:47.20 AS.seek 0.545ml @00:19:12.64 delay 10 seconds @00:19:22.67 ATV.dial :air1 @00:19:24.40 AS.reconfigure ASslow1 @00:19:24.46 AS.seek 0.575ml @00:19:30.81 ATV.dial :waste @00:19:31.38 AS.reconfigure ASconfig @00:19:31.43 AS.seek :empty @00:20:04.83 AFV.select :carrierSolution @00:20:04.95 delay 2 seconds @00:20:06.97 AS.seek 0.5ml @00:20:36.09 delay 1 second @00:20:37.11 AFV.select :holdingCoil @00:20:37.22 ATV.dial :air1 @00:20:37.81 AS.reconfigure ASslow1 @00:20:37.87 AS.seek 0.52ml @00:20:42.17 delay 5 seconds @00:20:47.19 ATV.dial :extSrc @00:20:48.90 AS.reconfigure ASslow2 @00:20:48.95 AS.seek 0.538ml @00:21:07.23 delay 10 seconds @00:21:17.26 ATV.dial :air1 @00:21:19.00 AS.reconfigure ASslow1 @00:21:19.05 AS.seek 0.568ml @00:21:25.36 ATV.dial :waste @00:21:26.04 AS.reconfigure ASconfig @00:21:26.09 AS.seek :empty @00:21:59.11 deconEluateRecoveryLine extSrc @00:21:59.12 ATV.dial :waste @00:21:59.15 AS.reconfigure ASnorm @00:21:59.22 AS.seek 0ml @00:21:59.61 ATV.dial :air3 @00:22:00.29 AS.reconfigure ASslow1 @00:22:00.35 AS.seek 0.01ml @00:22:02.62 delay 2 seconds @00:22:04.64 ATV.dial :bleach @00:22:05.11 AS.seek 0.31ml @00:23:05.72 delay 3 seconds @00:23:08.74 ATV.dial :air3 @00:23:09.31 delay 3 seconds @00:23:12.38 AS.seek 0.335ml @00:23:17.69 delay 3 seconds @00:23:20.71 ATV.dial :bleach @00:23:21.36 AS.seek 0.32ml @00:23:25.05 delay 3 seconds @00:23:28.07 ATV.dial :extSrc @00:23:28.70 AS.seek 0ml @00:24:33.33 AFV.select :carrierSolution @00:24:33.45 delay 2 seconds @00:24:35.47 AS.reconfigure ASconfig @00:24:35.53 AS.seek 0.5ml @00:25:04.58 delay 1 second @00:25:05.60 AFV.select :holdingCoil @00:25:05.70 ATV.dial :extSrc @00:25:05.72 AS.reconfigure ASslow1 @00:25:05.79 AS.seek 0ml @00:26:46.62 AFV.select :carrierSolution @00:26:46.74 delay 2 seconds @00:26:48.76 AS.reconfigure ASconfig @00:26:48.82 AS.seek 0.5ml @00:27:17.86 delay 1 second @00:27:18.88 AFV.select :holdingCoil @00:27:18.98 ATV.dial :extSrc @00:27:19.00 AS.reconfigure ASslow1 @00:27:19.07 AS.seek 0ml @00:28:59.92 AFV.select :carrierSolution @00:29:00.04 delay 2 seconds @00:29:02.06 AS.reconfigure ASconfig @00:29:02.13 AS.seek 0.5ml @00:29:31.15 delay 1 second @00:29:32.17 AFV.select :holdingCoil @00:29:32.27 ATV.dial :extSrc @00:29:32.29 AS.reconfigure ASslow1 @00:29:32.37 AS.seek 0ml @00:31:13.17 AFV.select :carrierSolution @00:31:13.29 delay 2 seconds @00:31:15.31 AS.reconfigure ASconfig @00:31:15.37 AS.seek 0.5ml @00:31:44.41 delay 1 second @00:31:45.43 AFV.select :holdingCoil @00:31:45.53 ATV.dial :extSrc @00:31:45.55 AS.reconfigure ASslow1 @00:31:45.62 AS.seek 0ml @00:33:26.45 ATV.dial :air2 @00:33:27.79 AS.reconfigure ASslow0 @00:33:27.85 AS.seek 0.5ml @00:34:18.51 ATV.dial :extSrc @00:34:20.01 AS.seek 0.02ml @00:35:08.64 delay 1 second @00:35:09.67 ATV.dial :waste @00:35:10.95 AS.reconfigure ASconfig @00:35:11.00 AS.seek :empty @00:35:12.52 deconEluateRecoveryLine extSrc ATV.dial :waste @00:35:12.56 AS.reconfigure ASnorm @00:35:12.62 AS.seek 0ml @00:35:13.03 ATV.dial :air3 @00:35:13.88 AS.reconfigure ASslow1 @00:35:13.94 AS.seek 0.01ml @00:35:16.28 delay 2 seconds @00:35:18.30 ATV.dial :bleach @00:35:18.76 AS.seek 0.31ml @00:36:19.37 delay 3 seconds @00:36:22.39 ATV.dial :air3 @00:36:22.97 delay 3 seconds @00:36:26.04 AS.seek 0.335ml @00:36:31.35 delay 3 seconds @00:36:34.37 ATV.dial :bleach @00:36:35.01 AS.seek 0.32ml @00:36:38.37 delay 3 seconds @00:36:41.39 ATV.dial :extSrc @00:36:42.02 AS.seek 0ml @00:37:46.64 AFV.select :carrierSolution @00:37:46.76 delay 2 seconds @00:37:48.78 AS.reconfigure ASconfig @00:37:48.84 AS.seek 0.5ml @00:38:17.86 delay 1 second @00:38:18.88 AFV.select :holdingCoil @00:38:18.98 ATV.dial :extSrc @00:38:19.00 AS.reconfigure ASslow1 @00:38:19.08 AS.seek 0ml @00:39:59.92 AFV.select :carrierSolution @00:40:00.04 delay 2 seconds @00:40:02.06 AS.reconfigure ASconfig @00:40:02.11 AS.seek 0.5ml @00:40:31.14 delay 1 second @00:40:32.16 AFV.select :holdingCoil @00:40:32.26 ATV.dial :extSrc @00:40:32.28 AS.reconfigure ASslow1 @00:40:32.36 AS.seek 0ml @00:42:13.20 AFV.select :carrierSolution @00:42:13.32 delay 2 seconds @00:42:15.34 AS.reconfigure ASconfig @00:42:15.40 AS.seek 0.5ml @00:42:44.44 delay 1 second @00:42:45.46 AFV.select :holdingCoil @00:42:45.57 ATV.dial :extSrc @00:42:45.59 AS.reconfigure ASslow1 @00:42:45.65 AS.seek 0ml @00:44:26.50 AFV.select :carrierSolution @00:44:26.62 delay 2 seconds @00:44:28.64 AS.reconfigure ASconfig @00:44:28.70 AS.seek 0.5ml @00:44:57.73 delay 1 second @00:44:58.75 AFV.select :holdingCoil @00:44:58.85 ATV.dial :extSrc @00:44:58.87 AS.reconfigure ASslow1 @00:44:58.95 AS.seek 0ml @00:46:39.80 ATV.dial :air2 @00:46:41.14 AS.reconfigure ASslow0 @00:46:41.20 AS.seek 0.5ml @00:47:31.84 ATV.dial :extSrc @00:47:33.34 AS.seek 0.02ml @00:48:21.94 delay 1 second @00:48:22.97 ATV.dial :waste @00:48:24.24 AS.reconfigure ASconfig @00:48:24.29 AS.seek :empty @00:48:25.78 deconEluateRecoveryLine extSrc @00:48:25.79 ATV.dial :waste @00:48:26.13 AS.reconfigure ASnorm @00:48:26.26 AS.seek 0ml @00:48:26.59 ATV.dial :air3 @00:48:27.44 AS.reconfigure ASslow1 @00:48:27.50 AS.seek 0.01ml @00:48:29.78 delay 2 seconds @00:48:31.80 ATV.dial :bleach @00:48:32.26 AS.seek 0.31ml @00:49:32.84 delay 3 seconds @00:49:35.86 ATV.dial :air3 @00:49:36.42 delay 3 seconds @00:49:39.50 AS.seek 0.335ml @00:49:44.81 delay 3 seconds @00:49:47.83 ATV.dial :bleach @00:49:48.45 AS.seek 0.32ml @00:49:51.78 delay 3 seconds @00:49:54.80 ATV.dial :extSrc @00:49:55.43 AS.seek 0ml @00:51:00.05 AFV.select :carrierSolution @00:51:00.17 delay 2 seconds @00:51:02.19 AS.reconfigure ASconfig @00:51:02.25 AS.seek 0.5ml @00:51:31.28 delay 1 second @00:51:32.30 AFV.select :holdingCoil @00:51:32.40 ATV.dial :extSrc @00:51:32.42 AS.reconfigure ASslow1 @00:51:32.50 AS.seek 0ml @00:53:13.34 AFV.select :carrierSolution @00:53:13.46 delay 2 seconds @00:53:15.48 AS.reconfigure ASconfig @00:53:15.54 AS.seek 0.5ml @00:53:44.58 delay 1 second @00:53:45.60 AFV.select :holdingCoil @00:53:45.70 ATV.dial :extSrc @00:53:45.72 AS.reconfigure ASslow1 @00:53:45.80 AS.seek 0ml @00:55:26.62 AFV.select :carrierSolution @00:55:26.74 delay 2 seconds @00:55:28.76 AS.reconfigure ASconfig @00:55:28.82 AS.seek 0.5ml @00:55:57.83 delay 1 second @00:55:58.85 AFV.select :holdingCoil @00:55:58.95 ATV.dial :extSrc @00:55:58.97 AS.reconfigure ASslow1 @00:55:59.05 AS.seek 0ml @00:57:39.91 AFV.select :carrierSolution @00:57:40.03 delay 2 seconds @00:57:42.05 AS.reconfigure ASconfig @00:57:42.11 AS.seek 0.5ml @00:58:11.14 delay 1 second @00:58:12.16 AFV.select :holdingCoil @00:58:12.26 ATV.dial :extSrc @00:58:12.28 AS.reconfigure ASslow1 @00:58:12.36 AS.seek 0ml @00:59:53.20 ATV.dial :air2 @00:59:54.54 AS.reconfigure ASslow0 @00:59:54.60 AS.seek 0.5ml @01:00:45.23 ATV.dial :extSrc @01:00:46.73 AS.seek 0.02ml @01:01:35.33 delay 1 second @01:01:36.36 ATV.dial :waste @01:01:37.64 AS.reconfigure ASconfig @01:01:37.69 AS.seek :empty @01:01:39.24 Session Ended @15:40:15.08CEST23-Apr-20
ESP2 v3.30 I2C Gateway v3.06 for core bus @15:40:17.02 Copyright 2018 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @15:40:17.33 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @15:40:22.78 Polling Can every 10:00 @15:40:24.61 SPE.coast @15:40:24.67 SPE.configure SPEconfig @15:40:24.76 AS.reconfigure ASconfig @15:40:24.80 AS.hold @15:40:24.86 AS.configure ASconfig @15:40:24.96 SS.reconfigure SSconfig @15:40:25.00 SS.hold @15:40:25.06 SS.configure SSconfig @15:40:25.17 CC.reconfigure CCconfig @15:40:25.21 CC.hold @15:40:25.28 CC.configure CCconfig @15:40:25.37 CS.reconfigure CSconfig @15:40:25.42 CS.hold @15:40:25.50 CS.configure CSconfig @15:40:25.97 PC.reconfigure PCconfig @15:40:26.02 PC.hold @15:40:26.09 PC.configure PCconfig @15:40:26.18 PS.configure PSconfig @15:40:26.30 Forearm.reconfigure LooseForearm @15:40:26.34 Forearm.hold @15:40:26.39 Forearm.configure LooseForearm @15:40:26.48 Elbow.reconfigure LooseElbow @15:40:26.53 Elbow.hold @15:40:26.58 Elbow.configure LooseElbow @15:40:26.69 SC.reconfigure SCloose @15:40:26.74 SC.hold @15:40:26.80 SC.configure SCloose @15:40:26.89 SE.reconfigure SEconfig @15:40:26.94 SE.hold @15:40:27.00 SE.configure SEconfig @15:40:27.05 CH.coast @15:40:27.10 CH.configure CHconfig @15:40:27.13 PH.coast @15:40:27.19 PH.configure PHconfig @15:40:27.23 PCR.off @15:40:27.25 PCR.pidtemp= 50,0.1,0,300,25,25 @15:40:27.28 PCR.caltemp= 2.8814,0.0372,0 @15:40:27.31 All dwarf controllers running firmware version 3.75 @15:40:27.50 Cleanly exited at 15:39:53CEST23-Apr-20 @15:40:27.54 Waiting for espclients... @15:40:33.82 Session Begun Log::Locked in trapHandler -- Another process is already writing to /var/log/esp/real.log @15:40:39.44 -> showlog 10 @15:41:40.22 -> exit @15:41:40.24 Session Ended @15:47:06.84CEST23-Apr-20
>RESET< ESP2 v3.30 I2C Gateway v3.06 for core bus @15:47:11.51 Copyright 2018 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @15:47:11.99 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @15:47:18.63 Polling Can every 10:00 @15:47:18.72 Cleanly exited at 15:40:27CEST23-Apr-20 @15:47:18.76 Loading /home/esp/esp2/mission/stdMER2017.rb mission @15:47:18.81 Powering core bus @15:47:19.65 Session Begun @15:47:23.88
Gate.power :raw,:ON @15:47:24.54 SPE.coast @15:47:24.62 SPE.configure SPEconfig @15:47:24.72 AS.configure ASconfig @15:47:24.84 SS.configure SSconfig @15:47:24.95 CC.configure CCconfig @15:47:25.07 CS.configure CSconfig @15:47:25.56 PC.configure PCconfig @15:47:25.68 PS.configure PSconfig @15:47:25.81 Forearm.configure LooseForearm @15:47:25.92 Elbow.configure LooseElbow @15:47:26.06 SC.configure SCloose @15:47:26.17 SE.configure SEconfig @15:47:26.24 CH.coast @15:47:26.30 CH.configure CHconfig @15:47:26.34 PH.coast @15:47:26.43 PH.configure PHconfig @15:47:26.48 PCR.off @15:47:26.53 PCR.pidtemp= 50,0.1,0,300,25,25 @15:47:26.56 PCR.caltemp= 2.8814,0.0372,0 @15:47:26.61 All dwarf controllers running firmware version 3.75 @15:47:26.83 Intake.select :close @15:47:26.89 Exhaust.select :close @15:47:26.93 CTV.dial :air @15:47:27.04 PSV.dial :air @15:47:27.08 CSV.dial :waste1 @15:47:27.30 SSV.dial :spit,avoiding: :exhaust @15:47:27.32
Forearm.reconfigure LooseForearm @15:47:27.35 PS.reconfigure PSconfig @15:47:27.50 PS.home.seek 0ml @15:47:27.53
Forearm.home.seek :retract @15:47:27.73 CS.reconfigure CSfast @15:47:27.77 SS.reconfigure SSconfig @15:47:27.88 CS.home.seek 0ml @15:47:27.92 SS.home.seek 0ml @15:47:28.34 Hand.close @15:47:28.38
Elbow.jog 1500 @15:47:28.94 Elbow.reconfigure LooseElbow @15:47:29.01 Elbow.home.seek :clear @15:47:33.71 SE.reconfigure SEconfig @15:47:33.79 SE.home.seek :down @15:47:34.43 SC.reconfigure SCsloppy @15:47:34.50 SC.home.seek :initial @15:47:36.86 PS.coast @15:47:38.54
Forearm.seek :retract @15:47:38.63 Elbow.seek :hammer @15:47:39.86 Forearm.reconfigure TightForearm @15:47:39.93 Forearm.seek 5 + 350 counts @15:47:41.05 Forearm.seek :retract @15:47:42.26 CC.reconfigure CCconfig @15:47:42.27 PC.reconfigure PCconfig @15:47:42.38 PC.home.seek :open @15:47:42.41
CC.home.seek :open @15:47:45.42 Readying the MFB @15:47:45.43 Homing MFB Syringe @15:47:45.45 AFV.select :holdingCoil @15:47:45.62 ATV.dial :air2 @15:47:46.71 AS.reconfigure ASconfig @15:47:46.77 AS.home.seek :home @15:47:52.32 AFV.select :carrierSolution @15:47:52.44 delay 2 seconds @15:47:54.47 AS.seek 1ml @15:48:47.40 delay 1 second @15:48:48.42 AFV.select :holdingCoil @15:48:48.53 delay 1 second @15:48:49.55 ATV.dial :waste @15:48:50.80 AS.reconfigure ASnorm @15:48:50.86 AS.home.seek :empty @15:49:43.46 ATV.dial :waste @15:49:43.47 ARV.dial between carrier and air1 @15:49:43.59 ASV.dial :waste1 @15:49:43.68 AFV.select :carrierSolution @15:49:43.79 delay 2 seconds @15:49:45.82 AS.reconfigure ASconfig @15:49:45.88 AS.seek 0.5ml @15:50:14.96 delay 1 second @15:50:15.98 AFV.select :holdingCoil @15:50:16.09 ATV.dial :extSrc @15:50:17.44 AS.reconfigure ASslow1 @15:50:17.50 AS.seek 0.53ml @15:50:23.73 delay 5 seconds @15:50:28.83 AS.reconfigure ASslow2 @15:50:28.89 AS.seek 0.54ml @15:50:39.28 delay 20 seconds @15:50:59.37 AS.reconfigure ASslow1 @15:50:59.43 AS.seek 0.57ml @15:51:05.79 delay 5 seconds @15:51:10.89 AS.reconfigure ASslow2 @15:51:10.95 AS.seek 0.58ml @15:51:21.20 delay 20 seconds @15:51:41.29 AS.reconfigure ASslow1 @15:51:41.35 AS.seek 0.61ml @15:51:47.71 delay 5 seconds @15:51:52.81 AS.reconfigure ASslow2 @15:51:52.87 AS.seek 0.62ml @15:52:03.29 delay 20 seconds @15:52:23.38 AS.reconfigure ASslow1 @15:52:23.44 AS.seek 0.65ml @15:52:29.79 delay 5 seconds @15:52:34.89 AS.reconfigure ASslow2 @15:52:34.95 AS.seek 0.66ml @15:52:45.21 delay 20 seconds @15:53:05.25 AS.reconfigure ASslow1 @15:53:05.31 AS.seek 0.705ml @15:53:14.67 delay 10 seconds @15:53:24.70 ATV.dial :air1 @15:53:26.45 AS.seek 0.725ml @15:53:30.76 delay 3 seconds @15:53:33.78 ATV.dial :waste @15:53:34.36 AS.reconfigure ASslow0 @15:53:34.42 AS.seek :empty @15:54:47.90 Setting up PCR for ML -- writing /var/log/esp/ML20apr2315h.pcr @15:54:47.93 Building PCR Slug from extSrc with MM2 mix, PP4 primer @15:54:47.96 Priming with carrier @15:54:47.98 ATV.dial ARV @15:54:49.48 ARV.dial :carrier @15:54:49.86 AS.reconfigure ASnorm @15:54:49.93 AS.seek 0.499ml @15:55:15.49 AFV.select :carrierSolution @15:55:15.62 delay 2 seconds @15:55:17.66 AS.reconfigure ASconfig @15:55:17.73 AS.seek 1ml @15:55:46.79 delay 1 second @15:55:47.81 AFV.select :holdingCoil @15:55:47.93 delay 1 second @15:55:48.96 ATV.dial :waste @15:55:50.75 AS.reconfigure ASnorm @15:55:50.82 AS.seek :empty @15:56:41.57 AFV.select :carrierSolution @15:56:41.70 delay 2 seconds @15:56:43.74 AS.reconfigure ASconfig @15:56:43.80 AS.seek 0.5ml @15:57:12.95 delay 1 second @15:57:13.97 AFV.select :holdingCoil @15:57:14.10 Building Slug @15:57:14.11 ATV.dial :ARV @15:57:15.76 delay 2 seconds @15:57:17.79 ARV.dial :air1 @15:57:18.20 delay 2 seconds @15:57:20.29 AS.reconfigure ASslow2 @15:57:20.36 AS.seek 0.506ml @15:57:27.20 delay 2 seconds @15:57:29.22 ARV.dial :MM2 @15:57:30.73 delay 2 seconds @15:57:32.76 Adding MM2 @15:57:32.87 AS.seek 0.524ml @15:57:51.15 delay 25 seconds @15:58:16.18 ARV.dial :PP4 @15:58:16.58 Adding PP4 @15:58:16.59 delay 2 seconds @15:58:18.69 AS.seek 0.53ml @15:58:24.95 delay 10 seconds @15:58:34.98 ARV.dial :air3 @15:58:35.41 adding ARV air @15:58:35.42 delay 2 seconds @15:58:37.51 AS.seek 0.536ml @15:58:43.82 delay 5 seconds @15:58:48.85 ARV.dial :carrier @15:58:50.13 delay 7 seconds @15:58:57.22 AS.seek 0.594ml @15:59:55.70 delay 5 seconds @16:00:00.73 ATV.dial :extSrc @16:00:01.31 delay 2 seconds @16:00:03.40 AS.seek 0.6ml @16:00:09.71 delay 4 seconds @16:00:13.74 ATV.dial ARV @16:00:14.31 delay 2 seconds @16:00:16.34 ARV.dial :carrier @16:00:16.36 delay 2 seconds @16:00:18.45 AS.seek 0.65ml @16:01:09.02 delay 4 seconds @16:01:13.05 ATV.dial PCR @16:01:13.84 ASV.dial :waste1 @16:01:13.86 ARV.dial :air1 @16:01:14.31 Positioning Slug by 0.324ml @16:01:14.41 AS.seek 0.326ml @16:06:40.26 delay 5 seconds @16:06:45.29 ATV.dial ARV @16:06:46.09 ASV.dial :PCRlock @16:06:46.51 delay 5 seconds @16:06:51.54 Running ML Profile60C350 thermocycling @16:06:51.56 PCR.program Profile60C350 @16:06:51.69 Profile60C350 should complete in < 1:46:29 @16:06:51.71 PCR.run 2:18:06.25 @17:30:21.20 PCR.off @17:30:23.21 PCR for ML completed -- closed /var/log/esp/ML20apr2315h.pcr @17:30:23.30 Cleaning PCR @17:30:23.31 AFV.select :carrierSolution @17:30:23.44 delay 2 seconds @17:30:25.48 AS.reconfigure ASconfig @17:30:25.54 AS.seek 0.5ml @17:30:35.87 delay 1 second @17:30:36.89 AFV.select :holdingCoil @17:30:37.00 ASV.dial :waste1 @17:30:37.41 ATV.dial :air3 @17:30:38.73 AS.reconfigure ASslow1 @17:30:38.80 AS.seek 0.51ml @17:30:41.09 delay 2 seconds @17:30:43.11 ATV.dial :bleach @17:30:43.77 AS.seek 0.81ml @17:31:44.49 delay 3 seconds @17:31:47.52 ATV.dial :air3 @17:31:48.10 delay 3 seconds @17:31:51.19 AS.seek 0.835ml @17:31:56.52 delay 3 seconds @17:31:59.55 ATV.dial :bleach @17:32:00.20 AS.seek 0.82ml @17:32:03.51 delay 3 seconds @17:32:06.54 ARV.dial :waste @17:32:07.16 delay 3 seconds @17:32:10.19 ATV.dial :ARV @17:32:11.20 AS.reconfigure ASconfig @17:32:11.27 AS.seek 0.47ml @17:32:31.70 AFV.select :carrierSolution @17:32:31.82 delay 2 seconds @17:32:33.86 AS.seek 1ml @17:33:04.65 delay 1 second @17:33:05.67 AFV.select :holdingCoil @17:33:05.78 delay 1 second @17:33:06.82 AS.reconfigure ASfast @17:33:06.88 AS.seek 0ml @17:33:34.59 AFV.select :carrierSolution @17:33:34.72 delay 2 seconds @17:33:36.76 AS.reconfigure ASconfig @17:33:36.82 AS.seek 1ml @17:34:34.59 delay 1 second @17:34:35.61 AFV.select :holdingCoil @17:34:35.72 delay 1 second @17:34:36.76 AS.reconfigure ASfast @17:34:36.82 AS.seek 0ml @17:35:04.84 AFV.select :carrierSolution @17:35:04.96 delay 2 seconds @17:35:07.00 AS.reconfigure ASconfig @17:35:07.06 AS.seek 1ml @17:36:04.85 delay 1 second @17:36:05.87 AFV.select :holdingCoil @17:36:05.98 delay 1 second @17:36:07.02 AS.reconfigure ASfast @17:36:07.08 AS.seek 0ml @17:36:35.02 ARV.dial between carrier and air1 @17:36:35.54 ATV.dial :air3 @17:36:36.86 AS.reconfigure ASslow1 @17:36:36.93 AS.seek 0.01ml @17:36:39.24 delay 2 seconds @17:36:41.26 ATV.dial :bleach @17:36:41.91 AS.seek 0.81ml @17:39:23.04 delay 3 seconds @17:39:26.07 ATV.dial :air3 @17:39:26.65 delay 3 seconds @17:39:29.74 AS.seek 0.835ml @17:39:35.04 delay 3 seconds @17:39:38.07 ATV.dial :bleach @17:39:38.72 AS.seek 0.82ml @17:39:42.18 delay 3 seconds @17:39:45.21 ATV.dial PCR @17:39:45.63 AS.reconfigure ASconfig @17:39:45.69 AS.seek :empty @17:40:33.20 PCR.program :bleachProfile @17:40:33.26 PCR program should complete in < 2:45 @17:40:33.28 PCR.run 10:00 @17:43:29.06 PCR.off @17:43:29.11 AFV.select :carrierSolution @17:43:29.23 delay 2 seconds @17:43:31.27 AS.seek 1ml @17:44:29.10 delay 1 second @17:44:30.12 AFV.select :holdingCoil @17:44:30.23 delay 1 second @17:44:31.27 AS.reconfigure ASfast @17:44:31.33 AS.seek 0ml @17:44:58.98 delay 3 seconds @17:45:02.01 AFV.select :carrierSolution @17:45:02.12 delay 2 seconds @17:45:04.16 AS.reconfigure ASconfig @17:45:04.22 AS.seek 1ml @17:46:02.01 delay 1 second @17:46:03.03 AFV.select :holdingCoil @17:46:03.15 delay 1 second @17:46:04.19 AS.reconfigure ASfast @17:46:04.25 AS.seek 0ml @17:46:32.10 delay 3 seconds @17:46:35.13 AFV.select :carrierSolution @17:46:35.25 delay 2 seconds @17:46:37.29 AS.reconfigure ASconfig @17:46:37.36 AS.seek 1ml @17:47:35.12 delay 1 second @17:47:36.14 AFV.select :holdingCoil @17:47:36.25 delay 1 second @17:47:37.29 AS.reconfigure ASfast @17:47:37.35 AS.seek 0ml @17:48:05.01 delay 3 seconds @17:48:08.04 AFV.select :carrierSolution @17:48:08.16 delay 2 seconds @17:48:10.20 AS.reconfigure ASconfig @17:48:10.27 AS.seek 1ml @17:49:08.04 delay 1 second @17:49:09.06 AFV.select :holdingCoil @17:49:09.17 delay 1 second @17:49:10.21 AS.reconfigure ASfast @17:49:10.27 AS.seek 0ml @17:49:38.04 delay 3 seconds @17:49:41.07 AFV.select :carrierSolution @17:49:41.18 delay 2 seconds @17:49:43.22 AS.reconfigure ASconfig @17:49:43.29 AS.seek 1ml @17:50:41.07 delay 1 second @17:50:42.09 AFV.select :holdingCoil @17:50:42.20 delay 1 second @17:50:43.24 AS.reconfigure ASnorm @17:50:43.30 AS.seek 0ml @17:51:34.03
AFV.select :carrierSolution @17:51:34.15 delay 2 seconds @17:51:36.18 AS.reconfigure ASconfig @17:51:36.24 AS.seek 0.5ml @17:52:05.32 delay 1 second @17:52:06.34 AFV.select :holdingCoil @17:52:06.45 ATV.dial :air1 @17:52:07.98 AS.reconfigure ASslow2 @17:52:08.04 AS.seek 0.506ml @17:52:14.23 ATV.dial :extSrc @17:52:16.06 AS.seek 0.512ml @17:52:22.34 delay 5 seconds @17:52:27.37 ATV.dial :air1 @17:52:29.58 AS.reconfigure ASslow1 @17:52:29.65 AS.seek 0.542ml @17:52:36.05 ATV.dial :waste @17:52:36.63 AS.reconfigure ASconfig @17:52:36.69 AS.seek :empty @17:53:08.37 Setting up PCR for ML -- writing /var/log/esp/ML20apr2317h.pcr @17:53:08.40 Building PCR Slug from extSrc with MM2 mix, PP4 primer @17:53:08.43 Priming with carrier @17:53:08.45 ATV.dial ARV @17:53:09.95 ARV.dial :carrier @17:53:10.33 AS.reconfigure ASnorm @17:53:10.41 AS.seek 0.499ml @17:53:35.99 AFV.select :carrierSolution @17:53:36.12 delay 2 seconds @17:53:38.16 AS.reconfigure ASconfig @17:53:38.23 AS.seek 1ml @17:54:07.35 delay 1 second @17:54:08.37 AFV.select :holdingCoil @17:54:08.49 delay 1 second @17:54:09.52 ATV.dial :waste @17:54:11.21 AS.reconfigure ASnorm @17:54:11.28 AS.seek :empty @17:55:02.05 AFV.select :carrierSolution @17:55:02.18 delay 2 seconds @17:55:04.22 AS.reconfigure ASconfig @17:55:04.28 AS.seek 0.5ml @17:55:33.41 delay 1 second @17:55:34.43 AFV.select :holdingCoil @17:55:34.55 Building Slug @17:55:34.57 ATV.dial :ARV @17:55:36.34 delay 2 seconds @17:55:38.37 ARV.dial :air1 @17:55:38.79 delay 2 seconds @17:55:40.88 AS.reconfigure ASslow2 @17:55:40.95 AS.seek 0.506ml @17:55:47.26 delay 2 seconds @17:55:49.29 ARV.dial :MM2 @17:55:50.79 delay 2 seconds @17:55:52.82 Adding MM2 @17:55:52.92 AS.seek 0.524ml @17:56:11.30 delay 25 seconds @17:56:36.33 ARV.dial :PP4 @17:56:36.73 Adding PP4 @17:56:36.75 delay 2 seconds @17:56:38.84 AS.seek 0.53ml @17:56:45.20 delay 10 seconds @17:56:55.23 ARV.dial :air3 @17:56:55.65 adding ARV air @17:56:55.67 delay 2 seconds @17:56:57.76 AS.seek 0.536ml @17:57:04.16 delay 5 seconds @17:57:09.19 ARV.dial :carrier @17:57:10.48 delay 7 seconds @17:57:17.57 AS.seek 0.594ml @17:58:16.24 delay 5 seconds @17:58:21.27 ATV.dial :extSrc @17:58:21.85 delay 2 seconds @17:58:23.94 AS.seek 0.6ml @17:58:30.41 delay 4 seconds @17:58:34.44 ATV.dial ARV @17:58:35.01 delay 2 seconds @17:58:37.04 ARV.dial :carrier @17:58:37.06 delay 2 seconds @17:58:39.15 AS.seek 0.65ml @17:59:29.68 delay 4 seconds @17:59:33.71 ATV.dial PCR @17:59:34.49 ASV.dial :waste1 @17:59:34.51 ARV.dial :air1 @17:59:34.93 Positioning Slug by 0.324ml @17:59:35.03 AS.seek 0.326ml @18:05:01.51 delay 5 seconds @18:05:06.54 ATV.dial ARV @18:05:07.34 ASV.dial :PCRlock @18:05:07.76 delay 5 seconds @18:05:12.79 Running ML Profile60C350 thermocycling @18:05:12.81 PCR.program Profile60C350 @18:05:12.95 Profile60C350 should complete in < 1:46:29 @18:05:12.97 PCR.run 2:18:06.25 @19:28:30.55 PCR.off @19:28:32.16 PCR for ML completed -- closed /var/log/esp/ML20apr2317h.pcr @19:28:32.25 Cleaning PCR @19:28:32.26 AFV.select :carrierSolution @19:28:32.39 delay 2 seconds @19:28:34.43 AS.reconfigure ASconfig @19:28:34.49 AS.seek 0.5ml @19:28:44.79 delay 1 second @19:28:45.81 AFV.select :holdingCoil @19:28:45.93 ASV.dial :waste1 @19:28:46.33 ATV.dial :air3 @19:28:47.65 AS.reconfigure ASslow1 @19:28:47.72 AS.seek 0.51ml @19:28:50.12 delay 2 seconds @19:28:52.14 ATV.dial :bleach @19:28:52.80 AS.seek 0.81ml @19:29:53.41 delay 3 seconds @19:29:56.44 ATV.dial :air3 @19:29:57.02 delay 3 seconds @19:30:00.11 AS.seek 0.835ml @19:30:05.44 delay 3 seconds @19:30:08.47 ATV.dial :bleach @19:30:09.12 AS.seek 0.82ml @19:30:12.44 delay 3 seconds @19:30:15.47 ARV.dial :waste @19:30:16.10 delay 3 seconds @19:30:19.13 ATV.dial :ARV @19:30:20.05 AS.reconfigure ASconfig @19:30:20.12 AS.seek 0.47ml @19:30:40.55 AFV.select :carrierSolution @19:30:40.67 delay 2 seconds @19:30:42.71 AS.seek 1ml @19:31:13.46 delay 1 second @19:31:14.48 AFV.select :holdingCoil @19:31:14.59 delay 1 second @19:31:15.63 AS.reconfigure ASfast @19:31:15.69 AS.seek 0ml @19:31:43.55 AFV.select :carrierSolution @19:31:43.67 delay 2 seconds @19:31:45.71 AS.reconfigure ASconfig @19:31:45.77 AS.seek 1ml @19:32:43.55 delay 1 second @19:32:44.57 AFV.select :holdingCoil @19:32:44.68 delay 1 second @19:32:45.72 AS.reconfigure ASfast @19:32:45.78 AS.seek 0ml @19:33:13.54 AFV.select :carrierSolution @19:33:13.67 delay 2 seconds @19:33:15.71 AS.reconfigure ASconfig @19:33:15.77 AS.seek 1ml @19:34:13.55 delay 1 second @19:34:14.57 AFV.select :holdingCoil @19:34:14.69 delay 1 second @19:34:15.73 AS.reconfigure ASfast @19:34:15.79 AS.seek 0ml @19:34:43.65 ARV.dial between carrier and air1 @19:34:44.18 ATV.dial :air3 @19:34:45.51 AS.reconfigure ASslow1 @19:34:45.58 AS.seek 0.01ml @19:34:47.90 delay 2 seconds @19:34:49.92 ATV.dial :bleach @19:34:50.58 AS.seek 0.81ml @19:37:31.71 delay 3 seconds @19:37:34.74 ATV.dial :air3 @19:37:35.32 delay 3 seconds @19:37:38.41 AS.seek 0.835ml @19:37:43.72 delay 3 seconds @19:37:46.75 ATV.dial :bleach @19:37:47.40 AS.seek 0.82ml @19:37:50.71 delay 3 seconds @19:37:53.73 ATV.dial PCR @19:37:54.16 AS.reconfigure ASconfig @19:37:54.22 AS.seek :empty @19:38:41.69 PCR.program :bleachProfile @19:38:41.75 PCR program should complete in < 2:45 @19:38:41.76 PCR.run 10:00 @19:41:37.54 PCR.off @19:41:37.58 AFV.select :carrierSolution @19:41:37.70 delay 2 seconds @19:41:39.74 AS.seek 1ml @19:42:37.58 delay 1 second @19:42:38.60 AFV.select :holdingCoil @19:42:38.71 delay 1 second @19:42:39.75 AS.reconfigure ASfast @19:42:39.81 AS.seek 0ml @19:43:07.49 delay 3 seconds @19:43:10.51 AFV.select :carrierSolution @19:43:10.63 delay 2 seconds @19:43:12.67 AS.reconfigure ASconfig @19:43:12.74 AS.seek 1ml @19:44:10.52 delay 1 second @19:44:11.54 AFV.select :holdingCoil @19:44:11.65 delay 1 second @19:44:12.69 AS.reconfigure ASfast @19:44:12.75 AS.seek 0ml @19:44:40.38 delay 3 seconds @19:44:43.41 AFV.select :carrierSolution @19:44:43.53 delay 2 seconds @19:44:45.57 AS.reconfigure ASconfig @19:44:45.64 AS.seek 1ml @19:45:43.40 delay 1 second @19:45:44.42 AFV.select :holdingCoil @19:45:44.54 delay 1 second @19:45:45.58 AS.reconfigure ASfast @19:45:45.64 AS.seek 0ml @19:46:13.38 delay 3 seconds @19:46:16.41 AFV.select :carrierSolution @19:46:16.53 delay 2 seconds @19:46:18.57 AS.reconfigure ASconfig @19:46:18.64 AS.seek 1ml @19:47:16.43 delay 1 second @19:47:17.45 AFV.select :holdingCoil @19:47:17.57 delay 1 second @19:47:18.61 AS.reconfigure ASfast @19:47:18.67 AS.seek 0ml @19:47:46.26 delay 3 seconds @19:47:49.29 AFV.select :carrierSolution @19:47:49.41 delay 2 seconds @19:47:51.45 AS.reconfigure ASconfig @19:47:51.52 AS.seek 1ml @19:48:49.33 delay 1 second @19:48:50.35 AFV.select :holdingCoil @19:48:50.46 delay 1 second @19:48:51.50 AS.reconfigure ASnorm @19:48:51.56 AS.seek 0ml @19:49:42.28
AFV.select :carrierSolution @19:49:42.40 delay 2 seconds @19:49:44.44 AS.reconfigure ASconfig @19:49:44.50 AS.seek 0.5ml @19:50:13.58 delay 1 second @19:50:14.60 AFV.select :holdingCoil @19:50:14.71 ATV.dial :air1 @19:50:16.23 AS.reconfigure ASslow2 @19:50:16.29 AS.seek 0.51ml @19:50:26.76 delay 5 seconds @19:50:31.79 ATV.dial :extSrc @19:50:33.53 AS.seek 0.53ml @19:50:54.05 delay 10 seconds @19:51:04.08 ATV.dial :air1 @19:51:05.83 AS.reconfigure ASslow1 @19:51:05.89 AS.seek 0.56ml @19:51:12.21 ATV.dial :waste @19:51:12.78 AS.reconfigure ASconfig @19:51:12.84 AS.seek :empty @19:51:45.57 Setting up PCR for ML -- writing /var/log/esp/ML20apr2319h.pcr @19:51:45.60 Building PCR Slug from extSrc with MM2 mix, PP4 primer @19:51:45.62 Priming with carrier @19:51:45.65 ATV.dial ARV @19:51:47.15 ARV.dial :carrier @19:51:47.53 AS.reconfigure ASnorm @19:51:47.61 AS.seek 0.499ml @19:52:13.19 AFV.select :carrierSolution @19:52:13.32 delay 2 seconds @19:52:15.36 AS.reconfigure ASconfig @19:52:15.42 AS.seek 1ml @19:52:44.52 delay 1 second @19:52:45.54 AFV.select :holdingCoil @19:52:45.66 delay 1 second @19:52:46.69 ATV.dial :waste @19:52:48.39 AS.reconfigure ASnorm @19:52:48.46 AS.seek :empty @19:53:39.24 AFV.select :carrierSolution @19:53:39.37 delay 2 seconds @19:53:41.41 AS.reconfigure ASconfig @19:53:41.47 AS.seek 0.5ml @19:54:10.63 delay 1 second @19:54:11.65 AFV.select :holdingCoil @19:54:11.78 Building Slug @19:54:11.79 ATV.dial :ARV @19:54:13.46 delay 2 seconds @19:54:15.49 ARV.dial :air1 @19:54:15.89 delay 2 seconds @19:54:17.98 AS.reconfigure ASslow2 @19:54:18.05 AS.seek 0.506ml @19:54:24.51 delay 2 seconds @19:54:26.53 ARV.dial :MM2 @19:54:28.04 delay 2 seconds @19:54:30.07 Adding MM2 @19:54:30.16 AS.seek 0.524ml @19:54:48.69 delay 25 seconds @19:55:13.72 ARV.dial :PP4 @19:55:14.13 Adding PP4 @19:55:14.15 delay 2 seconds @19:55:16.24 AS.seek 0.53ml @19:55:22.73 delay 10 seconds @19:55:32.76 ARV.dial :air3 @19:55:33.16 adding ARV air @19:55:33.18 delay 2 seconds @19:55:35.28 AS.seek 0.536ml @19:55:41.68 delay 5 seconds @19:55:46.71 ARV.dial :carrier @19:55:48.01 delay 7 seconds @19:55:55.10 AS.seek 0.594ml @19:56:53.60 delay 5 seconds @19:56:58.63 ATV.dial :extSrc @19:56:59.21 delay 2 seconds @19:57:01.75 AS.seek 0.6ml @19:57:08.16 delay 4 seconds @19:57:12.19 ATV.dial ARV @19:57:12.76 delay 2 seconds @19:57:14.79 ARV.dial :carrier @19:57:14.81 delay 2 seconds @19:57:16.90 AS.seek 0.65ml @19:58:07.65 delay 4 seconds @19:58:11.68 ATV.dial PCR @19:58:12.46 ASV.dial :waste1 @19:58:12.48 ARV.dial :air1 @19:58:12.89 Positioning Slug by 0.324ml @19:58:13.00 AS.seek 0.326ml @20:03:39.23 delay 5 seconds @20:03:44.26 ATV.dial ARV @20:03:45.05 ASV.dial :PCRlock @20:03:45.48 delay 5 seconds @20:03:50.51 Running ML Profile60C350 thermocycling @20:03:50.53 PCR.program Profile60C350 @20:03:50.66 Profile60C350 should complete in < 1:46:29 @20:03:50.68 PCR.run 2:18:06.25 @21:27:09.29 PCR.off @21:27:10.90 PCR for ML completed -- closed /var/log/esp/ML20apr2319h.pcr @21:27:10.99 Cleaning PCR @21:27:11.00 AFV.select :carrierSolution @21:27:11.13 delay 2 seconds @21:27:13.17 AS.reconfigure ASconfig @21:27:13.23 AS.seek 0.5ml @21:27:23.57 delay 1 second @21:27:24.59 AFV.select :holdingCoil @21:27:24.70 ASV.dial :waste1 @21:27:25.12 ATV.dial :air3 @21:27:26.45 AS.reconfigure ASslow1 @21:27:26.52 AS.seek 0.51ml @21:27:28.79 delay 2 seconds @21:27:30.81 ATV.dial :bleach @21:27:31.92 AS.seek 0.81ml @21:28:32.52 delay 3 seconds @21:28:35.55 ATV.dial :air3 @21:28:36.12 delay 3 seconds @21:28:39.21 AS.seek 0.835ml @21:28:44.53 delay 3 seconds @21:28:47.56 ATV.dial :bleach @21:28:48.20 AS.seek 0.82ml @21:28:51.52 delay 3 seconds @21:28:54.55 ARV.dial :waste @21:28:55.18 delay 3 seconds @21:28:58.21 ATV.dial :ARV @21:28:59.12 AS.reconfigure ASconfig @21:28:59.19 AS.seek 0.47ml @21:29:19.60 AFV.select :carrierSolution @21:29:19.72 delay 2 seconds @21:29:21.76 AS.seek 1ml @21:29:52.55 delay 1 second @21:29:53.57 AFV.select :holdingCoil @21:29:53.69 delay 1 second @21:29:54.73 AS.reconfigure ASfast @21:29:54.79 AS.seek 0ml @21:30:22.57 AFV.select :carrierSolution @21:30:22.70 delay 2 seconds @21:30:24.74 AS.reconfigure ASconfig @21:30:24.80 AS.seek 1ml @21:31:22.58 delay 1 second @21:31:23.60 AFV.select :holdingCoil @21:31:23.72 delay 1 second @21:31:24.76 AS.reconfigure ASfast @21:31:24.82 AS.seek 0ml @21:31:52.52 AFV.select :carrierSolution @21:31:52.65 delay 2 seconds @21:31:54.69 AS.reconfigure ASconfig @21:31:54.75 AS.seek 1ml @21:32:52.51 delay 1 second @21:32:53.53 AFV.select :holdingCoil @21:32:53.64 delay 1 second @21:32:54.68 AS.reconfigure ASfast @21:32:54.74 AS.seek 0ml @21:33:22.66 ARV.dial between carrier and air1 @21:33:23.18 ATV.dial :air3 @21:33:24.51 AS.reconfigure ASslow1 @21:33:24.58 AS.seek 0.01ml @21:33:26.94 delay 2 seconds @21:33:28.96 ATV.dial :bleach @21:33:29.61 AS.seek 0.81ml @21:36:10.76 delay 3 seconds @21:36:13.79 ATV.dial :air3 @21:36:14.37 delay 3 seconds @21:36:17.46 AS.seek 0.835ml @21:36:22.78 delay 3 seconds @21:36:25.81 ATV.dial :bleach @21:36:26.47 AS.seek 0.82ml @21:36:29.80 delay 3 seconds @21:36:32.83 ATV.dial PCR @21:36:33.25 AS.reconfigure ASconfig @21:36:33.31 AS.seek :empty @21:37:20.79 PCR.program :bleachProfile @21:37:20.85 PCR program should complete in < 2:45 @21:37:20.87 PCR.run 10:00 @21:40:16.65 PCR.off @21:40:16.69 AFV.select :carrierSolution @21:40:16.80 delay 2 seconds @21:40:18.84 AS.seek 1ml @21:41:16.69 delay 1 second @21:41:17.71 AFV.select :holdingCoil @21:41:17.82 delay 1 second @21:41:18.86 AS.reconfigure ASfast @21:41:18.92 AS.seek 0ml @21:41:46.68 delay 3 seconds @21:41:49.71 AFV.select :carrierSolution @21:41:49.83 delay 2 seconds @21:41:51.87 AS.reconfigure ASconfig @21:41:51.93 AS.seek 1ml @21:42:49.74 delay 1 second @21:42:50.76 AFV.select :holdingCoil @21:42:50.88 delay 1 second @21:42:51.92 AS.reconfigure ASfast @21:42:51.98 AS.seek 0ml @21:43:19.65 delay 3 seconds @21:43:22.68 AFV.select :carrierSolution @21:43:22.80 delay 2 seconds @21:43:24.84 AS.reconfigure ASconfig @21:43:24.92 AS.seek 1ml @21:44:22.68 delay 1 second @21:44:23.70 AFV.select :holdingCoil @21:44:23.81 delay 1 second @21:44:24.85 AS.reconfigure ASfast @21:44:24.91 AS.seek 0ml @21:44:52.55 delay 3 seconds @21:44:55.58 AFV.select :carrierSolution @21:44:55.70 delay 2 seconds @21:44:57.74 AS.reconfigure ASconfig @21:44:57.81 AS.seek 1ml @21:45:55.58 delay 1 second @21:45:56.60 AFV.select :holdingCoil @21:45:56.71 delay 1 second @21:45:57.75 AS.reconfigure ASfast @21:45:57.81 AS.seek 0ml @21:46:25.91 delay 3 seconds @21:46:28.94 AFV.select :carrierSolution @21:46:29.06 delay 2 seconds @21:46:31.10 AS.reconfigure ASconfig @21:46:31.16 AS.seek 1ml @21:47:28.94 delay 1 second @21:47:29.96 AFV.select :holdingCoil @21:47:30.08 delay 1 second @21:47:31.12 AS.reconfigure ASnorm @21:47:31.18 AS.seek 0ml @21:48:21.92
AFV.select :carrierSolution @21:48:22.04 delay 2 seconds @21:48:24.07 AS.reconfigure ASconfig @21:48:24.13 AS.seek 0.5ml @21:48:53.16 delay 1 second @21:48:54.18 AFV.select :holdingCoil @21:48:54.29 ATV.dial :air1 @21:48:55.82 AS.reconfigure ASslow2 @21:48:55.88 AS.seek 0.506ml @21:49:02.49 ATV.dial :extSrc @21:49:04.22 AS.seek 0.512ml @21:49:10.51 delay 5 seconds @21:49:15.54 ATV.dial :air1 @21:49:17.30 AS.reconfigure ASslow1 @21:49:17.36 AS.seek 0.542ml @21:49:23.66 ATV.dial :waste @21:49:24.24 AS.reconfigure ASconfig @21:49:24.30 AS.seek :empty @21:49:55.98 Setting up PCR for ML -- writing /var/log/esp/ML20apr2321h.pcr @21:49:56.01 Building PCR Slug from extSrc with MM2 mix, PP4 primer @21:49:56.04 Priming with carrier @21:49:56.06 ATV.dial ARV @21:49:57.57 ARV.dial :carrier @21:49:57.95 AS.reconfigure ASnorm @21:49:58.02 AS.seek 0.499ml @21:50:23.62 AFV.select :carrierSolution @21:50:23.75 delay 2 seconds @21:50:25.79 AS.reconfigure ASconfig @21:50:25.86 AS.seek 1ml @21:50:54.94 delay 1 second @21:50:55.96 AFV.select :holdingCoil @21:50:56.09 delay 1 second @21:50:57.11 ATV.dial :waste @21:50:58.78 AS.reconfigure ASnorm @21:50:58.85 AS.seek :empty @21:51:49.60 AFV.select :carrierSolution @21:51:49.73 delay 2 seconds @21:51:51.77 AS.reconfigure ASconfig @21:51:51.83 AS.seek 0.5ml @21:52:20.96 delay 1 second @21:52:21.98 AFV.select :holdingCoil @21:52:22.10 Building Slug @21:52:22.12 ATV.dial :ARV @21:52:23.88 delay 2 seconds @21:52:25.91 ARV.dial :air1 @21:52:26.43 delay 2 seconds @21:52:28.52 AS.reconfigure ASslow2 @21:52:28.59 AS.seek 0.506ml @21:52:35.08 delay 2 seconds @21:52:37.11 ARV.dial :MM2 @21:52:38.63 delay 2 seconds @21:52:40.66 Adding MM2 @21:52:40.77 AS.seek 0.524ml @21:52:59.30 delay 25 seconds @21:53:24.33 ARV.dial :PP4 @21:53:24.74 Adding PP4 @21:53:24.75 delay 2 seconds @21:53:26.84 AS.seek 0.53ml @21:53:33.10 delay 10 seconds @21:53:43.13 ARV.dial :air3 @21:53:43.54 adding ARV air @21:53:43.56 delay 2 seconds @21:53:45.65 AS.seek 0.536ml @21:53:51.96 delay 5 seconds @21:53:56.99 ARV.dial :carrier @21:53:58.26 delay 7 seconds @21:54:05.35 AS.seek 0.594ml @21:55:04.16 delay 5 seconds @21:55:09.19 ATV.dial :extSrc @21:55:09.77 delay 2 seconds @21:55:11.86 AS.seek 0.6ml @21:55:18.26 delay 4 seconds @21:55:22.29 ATV.dial ARV @21:55:22.87 delay 2 seconds @21:55:24.90 ARV.dial :carrier @21:55:24.92 delay 2 seconds @21:55:27.01 AS.seek 0.65ml @21:56:17.58 delay 4 seconds @21:56:21.61 ATV.dial PCR @21:56:22.40 ASV.dial :waste1 @21:56:22.42 ARV.dial :air1 @21:56:22.95 Positioning Slug by 0.324ml @21:56:23.06 AS.seek 0.326ml @22:01:49.26 delay 5 seconds @22:01:54.29 ATV.dial ARV @22:01:55.48 ASV.dial :PCRlock @22:01:55.94 delay 5 seconds @22:02:00.97 Running ML Profile60C350 thermocycling @22:02:00.99 PCR.program Profile60C350 @22:02:01.12 Profile60C350 should complete in < 1:46:29 @22:02:01.14 PCR.run 2:18:06.25 @23:25:08.68 PCR.off @23:25:10.30 PCR for ML completed -- closed /var/log/esp/ML20apr2321h.pcr @23:25:10.39 Cleaning PCR @23:25:10.40 AFV.select :carrierSolution @23:25:10.53 delay 2 seconds @23:25:12.57 AS.reconfigure ASconfig @23:25:12.63 AS.seek 0.5ml @23:25:22.96 delay 1 second @23:25:23.98 AFV.select :holdingCoil @23:25:24.09 ASV.dial :waste1 @23:25:24.51 ATV.dial :air3 @23:25:25.84 AS.reconfigure ASslow1 @23:25:25.91 AS.seek 0.51ml @23:25:28.21 delay 2 seconds @23:25:30.23 ATV.dial :bleach @23:25:30.89 AS.seek 0.81ml @23:26:31.51 delay 3 seconds @23:26:34.54 ATV.dial :air3 @23:26:35.12 delay 3 seconds @23:26:38.21 AS.seek 0.835ml @23:26:43.52 delay 3 seconds @23:26:46.55 ATV.dial :bleach @23:26:47.20 AS.seek 0.82ml @23:26:50.54 delay 3 seconds @23:26:53.57 ARV.dial :waste @23:26:54.18 delay 3 seconds @23:26:57.21 ATV.dial :ARV @23:26:58.12 AS.reconfigure ASconfig @23:26:58.19 AS.seek 0.47ml @23:27:18.63 AFV.select :carrierSolution @23:27:18.76 delay 2 seconds @23:27:21.25 AS.seek 1ml @23:27:52.01 delay 1 second @23:27:53.03 AFV.select :holdingCoil @23:27:53.15 delay 1 second @23:27:54.19 AS.reconfigure ASfast @23:27:54.25 AS.seek 0ml @23:28:21.88 AFV.select :carrierSolution @23:28:22.01 delay 2 seconds @23:28:24.05 AS.reconfigure ASconfig @23:28:24.11 AS.seek 1ml @23:29:21.91 delay 1 second @23:29:22.93 AFV.select :holdingCoil @23:29:23.05 delay 1 second @23:29:24.09 AS.reconfigure ASfast @23:29:24.15 AS.seek 0ml @23:29:51.76 AFV.select :carrierSolution @23:29:51.89 delay 2 seconds @23:29:53.93 AS.reconfigure ASconfig @23:29:53.99 AS.seek 1ml @23:30:51.74 delay 1 second @23:30:52.76 AFV.select :holdingCoil @23:30:52.88 delay 1 second @23:30:53.92 AS.reconfigure ASfast @23:30:53.98 AS.seek 0ml @23:31:21.58 ARV.dial between carrier and air1 @23:31:22.08 ATV.dial :air3 @23:31:23.42 AS.reconfigure ASslow1 @23:31:23.49 AS.seek 0.01ml @23:31:25.84 delay 2 seconds @23:31:27.86 ATV.dial :bleach @23:31:28.51 AS.seek 0.81ml @23:34:09.66 delay 3 seconds @23:34:12.69 ATV.dial :air3 @23:34:13.27 delay 3 seconds @23:34:16.36 AS.seek 0.835ml @23:34:21.77 delay 3 seconds @23:34:24.80 ATV.dial :bleach @23:34:25.45 AS.seek 0.82ml @23:34:28.76 delay 3 seconds @23:34:31.79 ATV.dial PCR @23:34:32.20 AS.reconfigure ASconfig @23:34:32.26 AS.seek :empty @23:35:19.74 PCR.program :bleachProfile @23:35:19.80 PCR program should complete in < 2:45 @23:35:19.82 PCR.run 10:00 @23:38:15.58 PCR.off @23:38:15.62 AFV.select :carrierSolution @23:38:15.74 delay 2 seconds @23:38:17.78 AS.seek 1ml @23:39:15.63 delay 1 second @23:39:16.65 AFV.select :holdingCoil @23:39:16.76 delay 1 second @23:39:17.80 AS.reconfigure ASfast @23:39:17.86 AS.seek 0ml @23:39:45.52 delay 3 seconds @23:39:48.55 AFV.select :carrierSolution @23:39:48.67 delay 2 seconds @23:39:50.71 AS.reconfigure ASconfig @23:39:50.78 AS.seek 1ml @23:40:48.57 delay 1 second @23:40:49.59 AFV.select :holdingCoil @23:40:49.71 delay 1 second @23:40:50.75 AS.reconfigure ASfast @23:40:50.81 AS.seek 0ml @23:41:18.46 delay 3 seconds @23:41:21.49 AFV.select :carrierSolution @23:41:21.61 delay 2 seconds @23:41:23.65 AS.reconfigure ASconfig @23:41:23.72 AS.seek 1ml @23:42:21.48 delay 1 second @23:42:22.50 AFV.select :holdingCoil @23:42:22.61 delay 1 second @23:42:23.66 AS.reconfigure ASfast @23:42:23.72 AS.seek 0ml @23:42:51.30 delay 3 seconds @23:42:54.33 AFV.select :carrierSolution @23:42:54.45 delay 2 seconds @23:42:56.49 AS.reconfigure ASconfig @23:42:56.56 AS.seek 1ml @23:43:54.37 delay 1 second @23:43:55.39 AFV.select :holdingCoil @23:43:55.51 delay 1 second @23:43:56.55 AS.reconfigure ASfast @23:43:56.61 AS.seek 0ml @23:44:24.18 delay 3 seconds @23:44:27.21 AFV.select :carrierSolution @23:44:27.33 delay 2 seconds @23:44:29.37 AS.reconfigure ASconfig @23:44:29.44 AS.seek 1ml @23:45:27.24 delay 1 second @23:45:28.26 AFV.select :holdingCoil @23:45:28.37 delay 1 second @23:45:29.41 AS.reconfigure ASnorm @23:45:29.47 AS.seek 0ml @23:46:20.24
AFV.select :carrierSolution @23:46:20.36 delay 2 seconds @23:46:22.39 AS.reconfigure ASconfig @23:46:22.45 AS.seek 0.5ml @23:46:51.49 delay 1 second @23:46:52.51 AFV.select :holdingCoil @23:46:52.62 ATV.dial :air1 @23:46:54.15 AS.reconfigure ASslow1 @23:46:54.21 AS.seek 0.52ml @23:46:58.52 delay 5 seconds @23:47:03.55 ATV.dial :extSrc @23:47:05.30 AS.reconfigure ASslow2 @23:47:05.81 AS.seek 0.545ml @23:47:31.21 delay 10 seconds @23:47:41.24 ATV.dial :air1 @23:47:42.98 AS.reconfigure ASslow1 @23:47:43.05 AS.seek 0.575ml @23:47:49.35 ATV.dial :waste @23:47:49.92 AS.reconfigure ASconfig @23:47:49.98 AS.seek :empty @23:48:23.59 Setting up PCR for ML -- writing /var/log/esp/ML20apr2323h.pcr @23:48:23.62 Building PCR Slug from extSrc with MM2 mix, PP4 primer @23:48:23.65 Priming with carrier @23:48:23.67 ATV.dial ARV @23:48:25.18 ARV.dial :carrier @23:48:25.56 AS.reconfigure ASnorm @23:48:25.64 AS.seek 0.499ml @23:48:51.23 AFV.select :carrierSolution @23:48:51.36 delay 2 seconds @23:48:53.40 AS.reconfigure ASconfig @23:48:53.47 AS.seek 1ml @23:49:22.58 delay 1 second @23:49:23.61 AFV.select :holdingCoil @23:49:23.73 delay 1 second @23:49:24.76 ATV.dial :waste @23:49:26.55 AS.reconfigure ASnorm @23:49:26.62 AS.seek :empty @23:50:17.38 AFV.select :carrierSolution @23:50:17.51 delay 2 seconds @23:50:19.55 AS.reconfigure ASconfig @23:50:19.61 AS.seek 0.5ml @23:50:48.74 delay 1 second @23:50:49.76 AFV.select :holdingCoil @23:50:49.88 Building Slug @23:50:49.90 ATV.dial :ARV @23:50:51.55 delay 2 seconds @23:50:53.58 ARV.dial :air1 @23:50:54.00 delay 2 seconds @23:50:56.09 AS.reconfigure ASslow2 @23:50:56.16 AS.seek 0.506ml @23:51:02.38 delay 2 seconds @23:51:04.41 ARV.dial :MM2 @23:51:05.91 delay 2 seconds @23:51:07.94 Adding MM2 @23:51:08.05 AS.seek 0.524ml @23:51:26.40 delay 25 seconds @23:51:51.43 ARV.dial :PP4 @23:51:51.83 Adding PP4 @23:51:51.85 delay 2 seconds @23:51:53.94 AS.seek 0.53ml @23:52:00.38 delay 10 seconds @23:52:10.41 ARV.dial :air3 @23:52:10.80 adding ARV air @23:52:10.82 delay 2 seconds @23:52:12.91 AS.seek 0.536ml @23:52:19.12 delay 5 seconds @23:52:24.15 ARV.dial :carrier @23:52:25.44 delay 7 seconds @23:52:32.53 AS.seek 0.594ml @23:53:31.02 delay 5 seconds @23:53:36.05 ATV.dial :extSrc @23:53:36.63 delay 2 seconds @23:53:38.72 AS.seek 0.6ml @23:53:45.19 delay 4 seconds @23:53:49.22 ATV.dial ARV @23:53:49.79 delay 2 seconds @23:53:51.82 ARV.dial :carrier @23:53:51.84 delay 2 seconds @23:53:53.93 AS.seek 0.65ml @23:54:44.48 delay 4 seconds @23:54:48.51 ATV.dial PCR @23:54:49.30 ASV.dial :waste1 @23:54:49.32 ARV.dial :air1 @23:54:49.75 Positioning Slug by 0.324ml @23:54:49.85 AS.seek 0.326ml @00:00:16.12CEST24-Apr-20 delay 5 seconds @00:00:21.15 ATV.dial ARV @00:00:21.94 ASV.dial :PCRlock @00:00:22.35 delay 5 seconds @00:00:27.38 Running ML Profile60C350 thermocycling @00:00:27.40 PCR.program Profile60C350 @00:00:27.53 Profile60C350 should complete in < 1:46:29 @00:00:27.55 PCR.run 2:18:06.25 @01:23:38.76 PCR.off @01:23:40.36 PCR for ML completed -- closed /var/log/esp/ML20apr2323h.pcr @01:23:40.45 Cleaning PCR @01:23:40.46 AFV.select :carrierSolution @01:23:40.59 delay 2 seconds @01:23:42.63 AS.reconfigure ASconfig @01:23:42.69 AS.seek 0.5ml @01:23:53.04 delay 1 second @01:23:54.06 AFV.select :holdingCoil @01:23:54.17 ASV.dial :waste1 @01:23:54.58 ATV.dial :air3 @01:23:55.89 AS.reconfigure ASslow1 @01:23:55.96 AS.seek 0.51ml @01:23:58.28 delay 2 seconds @01:24:00.30 ATV.dial :bleach @01:24:00.94 AS.seek 0.81ml @01:25:01.55 delay 3 seconds @01:25:04.58 ATV.dial :air3 @01:25:05.16 delay 3 seconds @01:25:08.25 AS.seek 0.835ml @01:25:13.59 delay 3 seconds @01:25:16.62 ATV.dial :bleach @01:25:17.32 AS.seek 0.82ml @01:25:20.69 delay 3 seconds @01:25:23.71 ARV.dial :waste @01:25:24.33 delay 3 seconds @01:25:27.36 ATV.dial :ARV @01:25:28.28 AS.reconfigure ASconfig @01:25:28.35 AS.seek 0.47ml @01:25:48.77 AFV.select :carrierSolution @01:25:48.89 delay 2 seconds @01:25:50.93 AS.seek 1ml @01:26:21.68 delay 1 second @01:26:22.70 AFV.select :holdingCoil @01:26:22.81 delay 1 second @01:26:23.85 AS.reconfigure ASfast @01:26:23.91 AS.seek 0ml @01:26:51.48 AFV.select :carrierSolution @01:26:51.60 delay 2 seconds @01:26:53.64 AS.reconfigure ASconfig @01:26:53.70 AS.seek 1ml @01:27:51.46 delay 1 second @01:27:52.48 AFV.select :holdingCoil @01:27:52.59 delay 1 second @01:27:53.63 AS.reconfigure ASfast @01:27:53.69 AS.seek 0ml @01:28:21.32 AFV.select :carrierSolution @01:28:21.44 delay 2 seconds @01:28:23.48 AS.reconfigure ASconfig @01:28:23.54 AS.seek 1ml @01:29:21.30 delay 1 second @01:29:22.32 AFV.select :holdingCoil @01:29:22.43 delay 1 second @01:29:23.47 AS.reconfigure ASfast @01:29:23.53 AS.seek 0ml @01:29:51.33 ARV.dial between carrier and air1 @01:29:51.83 ATV.dial :air3 @01:29:53.17 AS.reconfigure ASslow1 @01:29:53.24 AS.seek 0.01ml @01:29:55.57 delay 2 seconds @01:29:57.59 ATV.dial :bleach @01:29:58.25 AS.seek 0.81ml @01:32:39.48 delay 3 seconds @01:32:42.51 ATV.dial :air3 @01:32:43.08 delay 3 seconds @01:32:46.17 AS.seek 0.835ml @01:32:51.47 delay 3 seconds @01:32:54.50 ATV.dial :bleach @01:32:55.15 AS.seek 0.82ml @01:32:58.46 delay 3 seconds @01:33:01.49 ATV.dial PCR @01:33:01.91 AS.reconfigure ASconfig @01:33:01.97 AS.seek :empty @01:33:49.46 PCR.program :bleachProfile @01:33:49.53 PCR program should complete in < 2:45 @01:33:49.54 PCR.run 10:00 @01:36:45.33 PCR.off @01:36:45.37 AFV.select :carrierSolution @01:36:45.48 delay 2 seconds @01:36:47.52 AS.seek 1ml @01:37:45.37 delay 1 second @01:37:46.39 AFV.select :holdingCoil @01:37:46.51 delay 1 second @01:37:47.55 AS.reconfigure ASfast @01:37:47.61 AS.seek 0ml @01:38:14.96 delay 3 seconds @01:38:17.99 AFV.select :carrierSolution @01:38:18.11 delay 2 seconds @01:38:20.15 AS.reconfigure ASconfig @01:38:20.21 AS.seek 1ml @01:39:18.01 delay 1 second @01:39:19.03 AFV.select :holdingCoil @01:39:19.15 delay 1 second @01:39:20.19 AS.reconfigure ASfast @01:39:20.25 AS.seek 0ml @01:39:47.62 delay 3 seconds @01:39:50.65 AFV.select :carrierSolution @01:39:50.77 delay 2 seconds @01:39:52.81 AS.reconfigure ASconfig @01:39:52.88 AS.seek 1ml @01:40:50.66 delay 1 second @01:40:51.68 AFV.select :holdingCoil @01:40:51.79 delay 1 second @01:40:52.83 AS.reconfigure ASfast @01:40:52.89 AS.seek 0ml @01:41:20.23 delay 3 seconds @01:41:23.26 AFV.select :carrierSolution @01:41:23.38 delay 2 seconds @01:41:25.42 AS.reconfigure ASconfig @01:41:25.49 AS.seek 1ml @01:42:23.27 delay 1 second @01:42:24.29 AFV.select :holdingCoil @01:42:24.40 delay 1 second @01:42:25.44 AS.reconfigure ASfast @01:42:25.50 AS.seek 0ml @01:42:52.85 delay 3 seconds @01:42:55.88 AFV.select :carrierSolution @01:42:56.00 delay 2 seconds @01:42:58.04 AS.reconfigure ASconfig @01:42:58.10 AS.seek 1ml @01:43:55.96 delay 1 second @01:43:56.98 AFV.select :holdingCoil @01:43:57.10 delay 1 second @01:43:58.14 AS.reconfigure ASnorm @01:43:58.20 AS.seek 0ml @01:44:48.94
AFV.select :carrierSolution @01:44:49.06 delay 2 seconds @01:44:51.09 AS.reconfigure ASconfig @01:44:51.15 AS.seek 0.5ml @01:45:20.19 delay 1 second @01:45:21.21 AFV.select :holdingCoil @01:45:21.32 ATV.dial :air1 @01:45:22.84 AS.reconfigure ASslow2 @01:45:22.90 AS.seek 0.506ml @01:45:29.51 ATV.dial :extSrc @01:45:31.34 AS.seek 0.512ml @01:45:37.62 delay 5 seconds @01:45:42.65 ATV.dial :air1 @01:45:44.39 AS.reconfigure ASslow1 @01:45:44.45 AS.seek 0.542ml @01:45:50.76 ATV.dial :waste @01:45:51.34 AS.reconfigure ASconfig @01:45:51.40 AS.seek :empty @01:46:23.08 Setting up PCR for ML -- writing /var/log/esp/ML20apr2401h.pcr @01:46:23.11 Building PCR Slug from extSrc with MM2 mix, PP4 primer @01:46:23.14 Priming with carrier @01:46:23.16 ATV.dial ARV @01:46:24.66 ARV.dial :carrier @01:46:25.03 AS.reconfigure ASnorm @01:46:25.11 AS.seek 0.499ml @01:46:50.71 AFV.select :carrierSolution @01:46:50.84 delay 2 seconds @01:46:53.32 AS.reconfigure ASconfig @01:46:53.39 AS.seek 1ml @01:47:22.52 delay 1 second @01:47:23.54 AFV.select :holdingCoil @01:47:23.68 delay 1 second @01:47:24.71 ATV.dial :waste @01:47:26.39 AS.reconfigure ASnorm @01:47:26.46 AS.seek :empty @01:48:17.24 AFV.select :carrierSolution @01:48:17.37 delay 2 seconds @01:48:19.41 AS.reconfigure ASconfig @01:48:19.47 AS.seek 0.5ml @01:48:48.60 delay 1 second @01:48:49.62 AFV.select :holdingCoil @01:48:49.74 Building Slug @01:48:49.76 ATV.dial :ARV @01:48:51.52 delay 2 seconds @01:48:53.55 ARV.dial :air1 @01:48:53.96 delay 2 seconds @01:48:56.05 AS.reconfigure ASslow2 @01:48:56.12 AS.seek 0.506ml @01:49:02.40 delay 2 seconds @01:49:04.43 ARV.dial :MM2 @01:49:05.93 delay 2 seconds @01:49:07.96 Adding MM2 @01:49:08.06 AS.seek 0.524ml @01:49:26.29 delay 25 seconds @01:49:51.32 ARV.dial :PP4 @01:49:51.73 Adding PP4 @01:49:51.75 delay 2 seconds @01:49:53.85 AS.seek 0.53ml @01:50:00.23 delay 10 seconds @01:50:10.26 ARV.dial :air3 @01:50:10.68 adding ARV air @01:50:10.70 delay 2 seconds @01:50:12.79 AS.seek 0.536ml @01:50:19.08 delay 5 seconds @01:50:24.11 ARV.dial :carrier @01:50:25.40 delay 7 seconds @01:50:32.49 AS.seek 0.594ml @01:51:31.13 delay 5 seconds @01:51:36.16 ATV.dial :extSrc @01:51:36.74 delay 2 seconds @01:51:38.83 AS.seek 0.6ml @01:51:45.23 delay 4 seconds @01:51:49.26 ATV.dial ARV @01:51:49.83 delay 2 seconds @01:51:51.86 ARV.dial :carrier @01:51:51.88 delay 2 seconds @01:51:53.97 AS.seek 0.65ml @01:52:44.44 delay 4 seconds @01:52:48.47 ATV.dial PCR @01:52:49.26 ASV.dial :waste1 @01:52:49.28 ARV.dial :air1 @01:52:49.72 Positioning Slug by 0.324ml @01:52:49.83 AS.seek 0.326ml @01:58:15.98 delay 5 seconds @01:58:21.01 ATV.dial ARV @01:58:21.80 ASV.dial :PCRlock @01:58:22.21 delay 5 seconds @01:58:27.24 Running ML Profile60C350 thermocycling @01:58:27.26 PCR.program Profile60C350 @01:58:27.39 Profile60C350 should complete in < 1:46:29 @01:58:27.41 PCR.run 2:18:06.25 @03:21:35.51 PCR.off @03:21:37.14 PCR for ML completed -- closed /var/log/esp/ML20apr2401h.pcr @03:21:37.23 Cleaning PCR @03:21:37.24 AFV.select :carrierSolution @03:21:37.37 delay 2 seconds @03:21:39.41 AS.reconfigure ASconfig @03:21:39.47 AS.seek 0.5ml @03:21:49.80 delay 1 second @03:21:50.82 AFV.select :holdingCoil @03:21:50.93 ASV.dial :waste1 @03:21:51.34 ATV.dial :air3 @03:21:52.67 AS.reconfigure ASslow1 @03:21:52.74 AS.seek 0.51ml @03:21:55.02 delay 2 seconds @03:21:57.04 ATV.dial :bleach @03:21:57.69 AS.seek 0.81ml @03:22:58.29 delay 3 seconds @03:23:01.32 ATV.dial :air3 @03:23:01.90 delay 3 seconds @03:23:04.99 AS.seek 0.835ml @03:23:10.35 delay 3 seconds @03:23:13.38 ATV.dial :bleach @03:23:14.03 AS.seek 0.82ml @03:23:17.37 delay 3 seconds @03:23:20.40 ARV.dial :waste @03:23:21.02 delay 3 seconds @03:23:24.05 ATV.dial :ARV @03:23:25.06 AS.reconfigure ASconfig @03:23:25.13 AS.seek 0.47ml @03:23:45.54 AFV.select :carrierSolution @03:23:45.67 delay 2 seconds @03:23:47.71 AS.seek 1ml @03:24:18.48 delay 1 second @03:24:19.50 AFV.select :holdingCoil @03:24:19.62 delay 1 second @03:24:20.66 AS.reconfigure ASfast @03:24:20.72 AS.seek 0ml @03:24:48.30 AFV.select :carrierSolution @03:24:48.43 delay 2 seconds @03:24:50.47 AS.reconfigure ASconfig @03:24:50.53 AS.seek 1ml @03:25:48.33 delay 1 second @03:25:49.35 AFV.select :holdingCoil @03:25:49.47 delay 1 second @03:25:50.51 AS.reconfigure ASfast @03:25:50.57 AS.seek 0ml @03:26:18.15 AFV.select :carrierSolution @03:26:18.28 delay 2 seconds @03:26:20.32 AS.reconfigure ASconfig @03:26:20.38 AS.seek 1ml @03:27:18.15 delay 1 second @03:27:19.17 AFV.select :holdingCoil @03:27:19.28 delay 1 second @03:27:20.32 AS.reconfigure ASfast @03:27:20.38 AS.seek 0ml @03:27:48.02 ARV.dial between carrier and air1 @03:27:48.54 ATV.dial :air3 @03:27:49.86 AS.reconfigure ASslow1 @03:27:49.92 AS.seek 0.01ml @03:27:52.30 delay 2 seconds @03:27:54.32 ATV.dial :bleach @03:27:54.97 AS.seek 0.81ml @03:30:36.10 delay 3 seconds @03:30:39.13 ATV.dial :air3 @03:30:39.71 delay 3 seconds @03:30:42.80 AS.seek 0.835ml @03:30:48.09 delay 3 seconds @03:30:51.12 ATV.dial :bleach @03:30:52.22 AS.seek 0.82ml @03:30:55.51 delay 3 seconds @03:30:58.54 ATV.dial PCR @03:30:58.95 AS.reconfigure ASconfig @03:30:59.01 AS.seek :empty @03:31:46.49 PCR.program :bleachProfile @03:31:46.56 PCR program should complete in < 2:45 @03:31:46.57 PCR.run 10:00 @03:34:42.35 PCR.off @03:34:42.39 AFV.select :carrierSolution @03:34:42.50 delay 2 seconds @03:34:44.54 AS.seek 1ml @03:35:42.43 delay 1 second @03:35:43.45 AFV.select :holdingCoil @03:35:43.57 delay 1 second @03:35:44.61 AS.reconfigure ASfast @03:35:44.67 AS.seek 0ml @03:36:12.24 delay 3 seconds @03:36:15.27 AFV.select :carrierSolution @03:36:15.39 delay 2 seconds @03:36:17.43 AS.reconfigure ASconfig @03:36:17.50 AS.seek 1ml @03:37:15.27 delay 1 second @03:37:16.29 AFV.select :holdingCoil @03:37:16.40 delay 1 second @03:37:17.44 AS.reconfigure ASfast @03:37:17.50 AS.seek 0ml @03:37:44.98 delay 3 seconds @03:37:48.01 AFV.select :carrierSolution @03:37:48.14 delay 2 seconds @03:37:50.18 AS.reconfigure ASconfig @03:37:50.24 AS.seek 1ml @03:38:48.02 delay 1 second @03:38:49.04 AFV.select :holdingCoil @03:38:49.15 delay 1 second @03:38:50.19 AS.reconfigure ASfast @03:38:50.25 AS.seek 0ml @03:39:17.62 delay 3 seconds @03:39:20.65 AFV.select :carrierSolution @03:39:20.77 delay 2 seconds @03:39:22.81 AS.reconfigure ASconfig @03:39:22.88 AS.seek 1ml @03:40:20.74 delay 1 second @03:40:21.76 AFV.select :holdingCoil @03:40:21.88 delay 1 second @03:40:22.92 AS.reconfigure ASfast @03:40:22.98 AS.seek 0ml @03:40:50.49 delay 3 seconds @03:40:53.52 AFV.select :carrierSolution @03:40:53.64 delay 2 seconds @03:40:55.68 AS.reconfigure ASconfig @03:40:55.75 AS.seek 1ml @03:41:53.57 delay 1 second @03:41:54.59 AFV.select :holdingCoil @03:41:54.70 delay 1 second @03:41:55.74 AS.reconfigure ASnorm @03:41:55.80 AS.seek 0ml @03:42:46.56
AFV.select :carrierSolution @03:42:46.68 delay 2 seconds @03:42:48.71 AS.reconfigure ASconfig @03:42:48.77 AS.seek 0.5ml @03:43:17.84 delay 1 second @03:43:18.86 AFV.select :holdingCoil @03:43:18.97 ATV.dial :air1 @03:43:20.50 AS.reconfigure ASslow1 @03:43:20.56 AS.seek 0.52ml @03:43:24.93 delay 5 seconds @03:43:29.96 ATV.dial :extSrc @03:43:31.80 AS.reconfigure ASslow2 @03:43:31.86 AS.seek 0.538ml @03:43:50.44 delay 10 seconds @03:44:00.47 ATV.dial :air1 @03:44:02.20 AS.reconfigure ASslow1 @03:44:02.26 AS.seek 0.568ml @03:44:08.60 ATV.dial :waste @03:44:09.17 AS.reconfigure ASconfig @03:44:09.23 AS.seek :empty @03:44:42.41 Setting up PCR for CG -- writing /var/log/esp/CG20apr2403h.pcr @03:44:42.44 Building PCR Slug from extSrc with MM1 mix, PP2 primer @03:44:42.47 Priming with carrier @03:44:42.49 ATV.dial ARV @03:44:43.99 ARV.dial :carrier @03:44:44.36 AS.reconfigure ASnorm @03:44:44.43 AS.seek 0.499ml @03:45:10.04 AFV.select :carrierSolution @03:45:10.17 delay 2 seconds @03:45:12.21 AS.reconfigure ASconfig @03:45:12.27 AS.seek 1ml @03:45:41.38 delay 1 second @03:45:42.40 AFV.select :holdingCoil @03:45:42.52 delay 1 second @03:45:43.55 ATV.dial :waste @03:45:45.24 AS.reconfigure ASnorm @03:45:45.31 AS.seek :empty @03:46:36.09 AFV.select :carrierSolution @03:46:36.22 delay 2 seconds @03:46:38.26 AS.reconfigure ASconfig @03:46:38.32 AS.seek 0.5ml @03:47:07.43 delay 1 second @03:47:08.45 AFV.select :holdingCoil @03:47:08.58 Building Slug @03:47:08.59 ATV.dial :ARV @03:47:10.24 delay 2 seconds @03:47:12.27 ARV.dial :air1 @03:47:12.67 delay 2 seconds @03:47:15.20 AS.reconfigure ASslow2 @03:47:15.27 AS.seek 0.506ml @03:47:21.54 delay 2 seconds @03:47:23.57 ARV.dial :MM1 @03:47:24.18 delay 2 seconds @03:47:26.21 Adding MM1 @03:47:26.30 AS.seek 0.524ml @03:47:44.73 delay 25 seconds @03:48:09.76 ARV.dial :PP2 @03:48:10.18 Adding PP2 @03:48:10.20 delay 2 seconds @03:48:12.30 AS.seek 0.53ml @03:48:18.91 delay 10 seconds @03:48:28.94 ARV.dial :air2 @03:48:29.34 adding ARV air @03:48:29.36 delay 2 seconds @03:48:31.45 AS.seek 0.536ml @03:48:37.74 delay 5 seconds @03:48:42.77 ARV.dial :carrier @03:48:44.07 delay 7 seconds @03:48:51.16 AS.seek 0.594ml @03:49:49.63 delay 5 seconds @03:49:54.66 ATV.dial :extSrc @03:49:55.24 delay 2 seconds @03:49:57.33 AS.seek 0.6ml @03:50:03.77 delay 4 seconds @03:50:07.80 ATV.dial ARV @03:50:08.37 delay 2 seconds @03:50:10.40 ARV.dial :carrier @03:50:10.42 delay 2 seconds @03:50:12.51 AS.seek 0.65ml @03:51:03.05 delay 4 seconds @03:51:07.08 ATV.dial PCR @03:51:07.87 ASV.dial :waste1 @03:51:07.89 ARV.dial :air1 @03:51:08.33 Positioning Slug by 0.324ml @03:51:08.44 AS.seek 0.326ml @03:56:34.59 delay 5 seconds @03:56:39.62 ATV.dial ARV @03:56:40.41 ASV.dial :PCRlock @03:56:40.84 delay 5 seconds @03:56:45.87 Running CG Profile60C300 thermocycling @03:56:45.89 PCR.program Profile60C300 @03:56:46.01 Profile60C300 should complete in < 1:40:53 @03:56:46.02 PCR.run 2:11:06.25 @05:18:41.36 PCR.off @05:18:41.95 PCR for CG completed -- closed /var/log/esp/CG20apr2403h.pcr @05:18:42.03 Cleaning PCR @05:18:42.04 AFV.select :carrierSolution @05:18:42.18 delay 2 seconds @05:18:44.22 AS.reconfigure ASconfig @05:18:44.28 AS.seek 0.5ml @05:18:54.62 delay 1 second @05:18:55.64 AFV.select :holdingCoil @05:18:55.76 ASV.dial :waste1 @05:18:56.16 ATV.dial :air3 @05:18:57.97 AS.reconfigure ASslow1 @05:18:58.04 AS.seek 0.51ml @05:19:00.45 delay 2 seconds @05:19:02.47 ATV.dial :bleach @05:19:03.12 AS.seek 0.81ml @05:20:03.76 delay 3 seconds @05:20:06.79 ATV.dial :air3 @05:20:07.37 delay 3 seconds @05:20:10.46 AS.seek 0.835ml @05:20:15.80 delay 3 seconds @05:20:18.83 ATV.dial :bleach @05:20:19.48 AS.seek 0.82ml @05:20:22.80 delay 3 seconds @05:20:25.83 ARV.dial :waste @05:20:26.46 delay 3 seconds @05:20:29.49 ATV.dial :ARV @05:20:30.41 AS.reconfigure ASconfig @05:20:30.47 AS.seek 0.47ml @05:20:50.90 AFV.select :carrierSolution @05:20:51.03 delay 2 seconds @05:20:53.07 AS.seek 1ml @05:21:23.80 delay 1 second @05:21:24.82 AFV.select :holdingCoil @05:21:24.94 delay 1 second @05:21:25.98 AS.reconfigure ASfast @05:21:26.04 AS.seek 0ml @05:21:53.68 AFV.select :carrierSolution @05:21:53.81 delay 2 seconds @05:21:55.85 AS.reconfigure ASconfig @05:21:55.91 AS.seek 1ml @05:22:53.70 delay 1 second @05:22:54.72 AFV.select :holdingCoil @05:22:54.84 delay 1 second @05:22:55.88 AS.reconfigure ASfast @05:22:55.94 AS.seek 0ml @05:23:23.63 AFV.select :carrierSolution @05:23:23.76 delay 2 seconds @05:23:25.80 AS.reconfigure ASconfig @05:23:25.86 AS.seek 1ml @05:24:23.62 delay 1 second @05:24:24.64 AFV.select :holdingCoil @05:24:24.75 delay 1 second @05:24:25.79 AS.reconfigure ASfast @05:24:25.85 AS.seek 0ml @05:24:53.84 ARV.dial between carrier and air1 @05:24:54.35 ATV.dial :air3 @05:24:55.69 AS.reconfigure ASslow1 @05:24:55.76 AS.seek 0.01ml @05:24:58.23 delay 2 seconds @05:25:00.25 ATV.dial :bleach @05:25:00.91 AS.seek 0.81ml @05:27:42.10 delay 3 seconds @05:27:45.13 ATV.dial :air3 @05:27:45.71 delay 3 seconds @05:27:48.80 AS.seek 0.835ml @05:27:54.16 delay 3 seconds @05:27:57.19 ATV.dial :bleach @05:27:57.84 AS.seek 0.82ml @05:28:01.10 delay 3 seconds @05:28:04.13 ATV.dial PCR @05:28:04.55 AS.reconfigure ASconfig @05:28:04.61 AS.seek :empty @05:28:52.10 PCR.program :bleachProfile @05:28:52.16 PCR program should complete in < 2:45 @05:28:52.18 PCR.run 10:00 @05:31:47.95 PCR.off @05:31:47.99 AFV.select :carrierSolution @05:31:48.10 delay 2 seconds @05:31:50.14 AS.seek 1ml @05:32:47.99 delay 1 second @05:32:49.01 AFV.select :holdingCoil @05:32:49.12 delay 1 second @05:32:50.16 AS.reconfigure ASfast @05:32:50.22 AS.seek 0ml @05:33:17.98 delay 3 seconds @05:33:21.01 AFV.select :carrierSolution @05:33:21.13 delay 2 seconds @05:33:23.17 AS.reconfigure ASconfig @05:33:23.23 AS.seek 1ml @05:34:21.01 delay 1 second @05:34:22.03 AFV.select :holdingCoil @05:34:22.15 delay 1 second @05:34:23.19 AS.reconfigure ASfast @05:34:23.25 AS.seek 0ml @05:34:51.09 delay 3 seconds @05:34:54.12 AFV.select :carrierSolution @05:34:54.24 delay 2 seconds @05:34:56.28 AS.reconfigure ASconfig @05:34:56.35 AS.seek 1ml @05:35:54.16 delay 1 second @05:35:55.18 AFV.select :holdingCoil @05:35:55.30 delay 1 second @05:35:56.34 AS.reconfigure ASfast @05:35:56.40 AS.seek 0ml @05:36:24.23 delay 3 seconds @05:36:27.26 AFV.select :carrierSolution @05:36:27.85 delay 2 seconds @05:36:29.89 AS.reconfigure ASconfig @05:36:29.96 AS.seek 1ml @05:37:27.74 delay 1 second @05:37:28.76 AFV.select :holdingCoil @05:37:28.88 delay 1 second @05:37:29.92 AS.reconfigure ASfast @05:37:29.99 AS.seek 0ml @05:37:57.73 delay 3 seconds @05:38:00.76 AFV.select :carrierSolution @05:38:00.88 delay 2 seconds @05:38:02.92 AS.reconfigure ASconfig @05:38:02.99 AS.seek 1ml @05:39:00.84 delay 1 second @05:39:01.86 AFV.select :holdingCoil @05:39:01.98 delay 1 second @05:39:03.02 AS.reconfigure ASnorm @05:39:03.08 AS.seek 0ml @05:39:53.80
AFV.select :carrierSolution @05:39:53.93 delay 2 seconds @05:39:55.96 AS.reconfigure ASconfig @05:39:56.02 AS.seek 0.5ml @05:40:25.05 delay 1 second @05:40:26.07 AFV.select :holdingCoil @05:40:26.19 ATV.dial :air1 @05:40:27.72 AS.reconfigure ASslow2 @05:40:27.79 AS.seek 0.506ml @05:40:34.09 ATV.dial :extSrc @05:40:35.92 AS.seek 0.512ml @05:40:42.32 delay 5 seconds @05:40:47.35 ATV.dial :air1 @05:40:49.09 AS.reconfigure ASslow1 @05:40:49.16 AS.seek 0.542ml @05:40:55.45 ATV.dial :waste @05:40:56.03 AS.reconfigure ASconfig @05:40:56.09 AS.seek :empty @05:41:27.79 Setting up PCR for IPc -- writing /var/log/esp/IPc20apr2405h.pcr @05:41:27.81 Building PCR Slug from elutionWater with MM3 mix, PP6 primer @05:41:27.84 Priming with carrier @05:41:27.87 ATV.dial ARV @05:41:29.37 ARV.dial :carrier @05:41:29.73 AS.reconfigure ASnorm @05:41:29.81 AS.seek 0.499ml @05:41:56.12 AFV.select :carrierSolution @05:41:56.25 delay 2 seconds @05:41:58.29 AS.reconfigure ASconfig @05:41:58.36 AS.seek 1ml @05:42:27.46 delay 1 second @05:42:28.48 AFV.select :holdingCoil @05:42:28.60 delay 1 second @05:42:29.63 ATV.dial :waste @05:42:31.33 AS.reconfigure ASnorm @05:42:31.40 AS.seek :empty @05:43:22.15 AFV.select :carrierSolution @05:43:22.28 delay 2 seconds @05:43:24.32 AS.reconfigure ASconfig @05:43:24.40 AS.seek 0.5ml @05:43:53.57 delay 1 second @05:43:54.59 AFV.select :holdingCoil @05:43:54.71 Building Slug @05:43:54.73 ATV.dial :ARV @05:43:56.41 delay 2 seconds @05:43:58.43 ARV.dial :air1 @05:43:58.94 delay 2 seconds @05:44:01.03 AS.reconfigure ASslow2 @05:44:01.11 AS.seek 0.506ml @05:44:07.41 delay 2 seconds @05:44:09.43 ARV.dial :MM3 @05:44:10.48 delay 2 seconds @05:44:12.51 Adding MM3 @05:44:12.62 AS.seek 0.524ml @05:44:30.85 delay 25 seconds @05:44:55.88 ARV.dial :PP6 @05:44:56.39 Adding PP6 @05:44:56.40 delay 2 seconds @05:44:58.49 AS.seek 0.53ml @05:45:04.73 delay 10 seconds @05:45:14.76 ARV.dial :air1 @05:45:15.62 adding ARV air @05:45:15.64 delay 2 seconds @05:45:17.73 AS.seek 0.536ml @05:45:23.99 delay 5 seconds @05:45:29.02 ARV.dial :carrier @05:45:29.45 delay 7 seconds @05:45:36.54 AS.seek 0.594ml @05:46:35.01 delay 5 seconds @05:46:40.04 ATV.dial :elutionWater @05:46:41.51 delay 2 seconds @05:46:43.60 AS.seek 0.6ml @05:46:49.84 delay 4 seconds @05:46:53.87 ATV.dial ARV @05:46:55.32 delay 2 seconds @05:46:57.35 ARV.dial :carrier @05:46:57.37 delay 2 seconds @05:46:59.46 AS.seek 0.65ml @05:47:50.10 delay 4 seconds @05:47:54.13 ATV.dial PCR @05:47:54.91 ASV.dial :waste1 @05:47:54.93 ARV.dial :air1 @05:47:55.81 Positioning Slug by 0.324ml @05:47:55.92 AS.seek 0.326ml @05:53:22.27 delay 5 seconds @05:53:27.30 ATV.dial ARV @05:53:28.10 ASV.dial :PCRlock @05:53:28.52 delay 5 seconds @05:53:33.55 Running IPc ProfileIPc thermocycling @05:53:33.57 PCR.program ProfileIPc @05:53:33.68 ProfileIPc should complete in < 1:40:53 @05:53:33.70 PCR.run 2:11:06.25 @07:16:13.21 PCR.off @07:16:13.80 PCR for IPc completed -- closed /var/log/esp/IPc20apr2405h.pcr @07:16:13.89 Cleaning PCR @07:16:13.91 AFV.select :carrierSolution @07:16:14.10 delay 2 seconds @07:16:16.14 AS.reconfigure ASconfig @07:16:16.20 AS.seek 0.5ml @07:16:26.52 delay 1 second @07:16:27.54 AFV.select :holdingCoil @07:16:27.66 ASV.dial :waste1 @07:16:28.07 ATV.dial :air3 @07:16:29.41 AS.reconfigure ASslow1 @07:16:29.48 AS.seek 0.51ml @07:16:31.88 delay 2 seconds @07:16:33.90 ATV.dial :bleach @07:16:34.55 AS.seek 0.81ml @07:17:35.16 delay 3 seconds @07:17:38.19 ATV.dial :air3 @07:17:38.77 delay 3 seconds @07:17:41.86 AS.seek 0.835ml @07:17:47.21 delay 3 seconds @07:17:50.24 ATV.dial :bleach @07:17:50.89 AS.seek 0.82ml @07:17:54.23 delay 3 seconds @07:17:57.25 ARV.dial :waste @07:17:57.88 delay 3 seconds @07:18:00.91 ATV.dial :ARV @07:18:01.83 AS.reconfigure ASconfig @07:18:01.90 AS.seek 0.47ml @07:18:22.31 AFV.select :carrierSolution @07:18:22.44 delay 2 seconds @07:18:24.48 AS.seek 1ml @07:18:55.27 delay 1 second @07:18:56.29 AFV.select :holdingCoil @07:18:56.40 delay 1 second @07:18:57.44 AS.reconfigure ASfast @07:18:57.50 AS.seek 0ml @07:19:25.10 AFV.select :carrierSolution @07:19:25.23 delay 2 seconds @07:19:27.27 AS.reconfigure ASconfig @07:19:27.33 AS.seek 1ml @07:20:25.10 delay 1 second @07:20:26.12 AFV.select :holdingCoil @07:20:26.24 delay 1 second @07:20:27.28 AS.reconfigure ASfast @07:20:27.34 AS.seek 0ml @07:20:55.01 AFV.select :carrierSolution @07:20:55.13 delay 2 seconds @07:20:57.17 AS.reconfigure ASconfig @07:20:57.71 AS.seek 1ml @07:21:55.52 delay 1 second @07:21:56.54 AFV.select :holdingCoil @07:21:56.66 delay 1 second @07:21:57.70 AS.reconfigure ASfast @07:21:57.76 AS.seek 0ml @07:22:25.41 ARV.dial between carrier and air1 @07:22:25.93 ATV.dial :air3 @07:22:27.27 AS.reconfigure ASslow1 @07:22:27.34 AS.seek 0.01ml @07:22:29.68 delay 2 seconds @07:22:31.70 ATV.dial :bleach @07:22:32.36 AS.seek 0.81ml @07:25:13.52 delay 3 seconds @07:25:16.55 ATV.dial :air3 @07:25:17.13 delay 3 seconds @07:25:20.22 AS.seek 0.835ml @07:25:25.53 delay 3 seconds @07:25:28.56 ATV.dial :bleach @07:25:29.31 AS.seek 0.82ml @07:25:32.65 delay 3 seconds @07:25:35.68 ATV.dial PCR @07:25:36.11 AS.reconfigure ASconfig @07:25:36.17 AS.seek :empty @07:26:23.67 PCR.program :bleachProfile @07:26:23.74 PCR program should complete in < 2:45 @07:26:23.75 PCR.run 10:00 @07:29:19.53 PCR.off @07:29:19.58 AFV.select :carrierSolution @07:29:19.69 delay 2 seconds @07:29:21.73 AS.seek 1ml @07:30:19.57 delay 1 second @07:30:20.60 AFV.select :holdingCoil @07:30:20.72 delay 1 second @07:30:21.76 AS.reconfigure ASfast @07:30:21.82 AS.seek 0ml @07:30:49.45 delay 3 seconds @07:30:52.48 AFV.select :carrierSolution @07:30:52.60 delay 2 seconds @07:30:54.64 AS.reconfigure ASconfig @07:30:54.71 AS.seek 1ml @07:31:52.48 delay 1 second @07:31:53.50 AFV.select :holdingCoil @07:31:53.61 delay 1 second @07:31:54.65 AS.reconfigure ASfast @07:31:54.71 AS.seek 0ml @07:32:22.40 delay 3 seconds @07:32:25.43 AFV.select :carrierSolution @07:32:25.55 delay 2 seconds @07:32:27.59 AS.reconfigure ASconfig @07:32:27.66 AS.seek 1ml @07:33:25.41 delay 1 second @07:33:26.43 AFV.select :holdingCoil @07:33:26.54 delay 1 second @07:33:27.58 AS.reconfigure ASfast @07:33:27.64 AS.seek 0ml @07:33:55.24 delay 3 seconds @07:33:58.27 AFV.select :carrierSolution @07:33:58.39 delay 2 seconds @07:34:00.43 AS.reconfigure ASconfig @07:34:00.50 AS.seek 1ml @07:34:58.27 delay 1 second @07:34:59.29 AFV.select :holdingCoil @07:34:59.41 delay 1 second @07:35:00.45 AS.reconfigure ASfast @07:35:00.51 AS.seek 0ml @07:35:28.32 delay 3 seconds @07:35:31.35 AFV.select :carrierSolution @07:35:31.47 delay 2 seconds @07:35:33.51 AS.reconfigure ASconfig @07:35:33.58 AS.seek 1ml @07:36:31.35 delay 1 second @07:36:32.37 AFV.select :holdingCoil @07:36:32.48 delay 1 second @07:36:33.52 AS.reconfigure ASnorm @07:36:33.58 AS.seek 0ml @07:37:24.34
deconEluateRecoveryLine extSrc @07:37:24.35 ATV.dial :waste @07:37:25.63 AS.seek 0ml @07:37:25.72 ATV.dial :air3 @07:37:26.56 AS.reconfigure ASslow1 @07:37:26.63 AS.seek 0.01ml @07:37:28.98 delay 2 seconds @07:37:31.00 ATV.dial :bleach @07:37:31.49 AS.seek 0.31ml @07:38:32.10 delay 3 seconds @07:38:35.12 ATV.dial :air3 @07:38:35.70 delay 3 seconds @07:38:38.79 AS.seek 0.335ml @07:38:44.13 delay 3 seconds @07:38:47.15 ATV.dial :bleach @07:38:47.80 AS.seek 0.32ml @07:38:51.10 delay 3 seconds @07:38:54.12 ATV.dial :extSrc @07:38:54.75 AS.seek 0ml @07:39:59.38 AFV.select :carrierSolution @07:39:59.50 delay 2 seconds @07:40:01.53 AS.reconfigure ASconfig @07:40:01.59 AS.seek 0.5ml @07:40:31.12 delay 1 second @07:40:32.14 AFV.select :holdingCoil @07:40:32.26 ATV.dial :extSrc @07:40:32.28 AS.reconfigure ASslow1 @07:40:32.36 AS.seek 0ml @07:42:13.21 AFV.select :carrierSolution @07:42:13.33 delay 2 seconds @07:42:15.36 AS.reconfigure ASconfig @07:42:15.42 AS.seek 0.5ml @07:42:44.45 delay 1 second @07:42:45.47 AFV.select :holdingCoil @07:42:45.59 ATV.dial :extSrc @07:42:45.61 AS.reconfigure ASslow1 @07:42:45.69 AS.seek 0ml @07:44:26.52 AFV.select :carrierSolution @07:44:26.64 delay 2 seconds @07:44:28.67 AS.reconfigure ASconfig @07:44:28.73 AS.seek 0.5ml @07:44:57.76 delay 1 second @07:44:58.78 AFV.select :holdingCoil @07:44:58.90 ATV.dial :extSrc @07:44:58.92 AS.reconfigure ASslow1 @07:44:59.00 AS.seek 0ml @07:46:39.84 AFV.select :carrierSolution @07:46:39.96 delay 2 seconds @07:46:41.99 AS.reconfigure ASconfig @07:46:42.05 AS.seek 0.5ml @07:47:11.07 delay 1 second @07:47:12.09 AFV.select :holdingCoil @07:47:12.21 ATV.dial :extSrc @07:47:12.23 AS.reconfigure ASslow1 @07:47:12.31 AS.seek 0ml @07:48:53.17 ATV.dial :air2 @07:48:54.53 AS.reconfigure ASslow0 @07:48:54.60 AS.seek 0.5ml @07:49:45.24 ATV.dial :extSrc @07:49:46.75 AS.seek 0.02ml @07:50:35.37 delay 1 second @07:50:36.39 ATV.dial :waste @07:50:37.71 AS.reconfigure ASconfig @07:50:37.77 AS.seek :empty @07:50:39.29 deconEluateRecoveryLine extSrc @07:50:39.30 ATV.dial :waste @07:50:39.34 AS.reconfigure ASnorm @07:50:39.41 AS.seek 0ml @07:50:39.81 ATV.dial :air3 @07:50:40.68 AS.reconfigure ASslow1 @07:50:40.75 AS.seek 0.01ml @07:50:43.12 delay 2 seconds @07:50:45.14 ATV.dial :bleach @07:50:45.64 AS.seek 0.31ml @07:51:46.29 delay 3 seconds @07:51:49.31 ATV.dial :air3 @07:51:49.88 delay 3 seconds @07:51:52.97 AS.seek 0.335ml @07:51:58.27 delay 3 seconds @07:52:01.29 ATV.dial :bleach @07:52:01.92 AS.seek 0.32ml @07:52:05.26 delay 3 seconds @07:52:08.28 ATV.dial :extSrc @07:52:08.93 AS.seek 0ml @07:53:13.56 AFV.select :carrierSolution @07:53:13.68 delay 2 seconds @07:53:15.71 AS.reconfigure ASconfig @07:53:15.77 AS.seek 0.5ml @07:53:44.82 delay 1 second @07:53:45.84 AFV.select :holdingCoil @07:53:45.96 ATV.dial :extSrc @07:53:45.98 AS.reconfigure ASslow1 @07:53:46.06 AS.seek 0ml @07:55:26.88 AFV.select :carrierSolution @07:55:27.00 delay 2 seconds @07:55:29.03 AS.reconfigure ASconfig @07:55:29.09 AS.seek 0.5ml @07:55:58.13 delay 1 second @07:55:59.15 AFV.select :holdingCoil @07:55:59.26 ATV.dial :extSrc @07:55:59.28 AS.reconfigure ASslow1 @07:55:59.36 AS.seek 0ml @07:57:40.21 AFV.select :carrierSolution @07:57:40.33 delay 2 seconds @07:57:42.36 AS.reconfigure ASconfig @07:57:42.42 AS.seek 0.5ml @07:58:11.46 delay 1 second @07:58:12.48 AFV.select :holdingCoil @07:58:12.59 ATV.dial :extSrc @07:58:12.61 AS.reconfigure ASslow1 @07:58:12.69 AS.seek 0ml @07:59:53.52 AFV.select :carrierSolution @07:59:53.64 delay 2 seconds @07:59:55.67 AS.reconfigure ASconfig @07:59:55.73 AS.seek 0.5ml @08:00:24.76 delay 1 second @08:00:25.78 AFV.select :holdingCoil @08:00:25.89 ATV.dial :extSrc @08:00:25.91 AS.reconfigure ASslow1 @08:00:25.99 AS.seek 0ml @08:02:06.87 ATV.dial :air2 @08:02:08.24 AS.reconfigure ASslow0 @08:02:08.30 AS.seek 0.5ml @08:02:58.92 ATV.dial :extSrc @08:03:00.44 AS.seek 0.02ml @08:03:48.99 delay 1 second @08:03:50.01 ATV.dial :waste @08:03:51.32 AS.reconfigure ASconfig @08:03:51.38 AS.seek :empty @08:03:52.88 deconEluateRecoveryLine extSrc @08:03:52.89 ATV.dial :waste @08:03:52.93 AS.reconfigure ASnorm @08:03:53.00 AS.seek 0ml @08:03:53.35 ATV.dial :air3 @08:03:54.21 AS.reconfigure ASslow1 @08:03:54.28 AS.seek 0.01ml @08:03:56.96 delay 2 seconds @08:03:58.98 ATV.dial :bleach @08:03:59.46 AS.seek 0.31ml @08:05:00.06 delay 3 seconds @08:05:03.08 ATV.dial :air3 @08:05:03.67 delay 3 seconds @08:05:06.76 AS.seek 0.335ml @08:05:12.10 delay 3 seconds @08:05:15.12 ATV.dial :bleach @08:05:15.77 AS.seek 0.32ml @08:05:19.07 delay 3 seconds @08:05:22.09 ATV.dial :extSrc @08:05:22.72 AS.seek 0ml @08:06:27.38 AFV.select :carrierSolution @08:06:27.50 delay 2 seconds @08:06:29.54 AS.reconfigure ASconfig @08:06:29.60 AS.seek 0.5ml @08:06:58.65 delay 1 second @08:06:59.67 AFV.select :holdingCoil @08:06:59.79 ATV.dial :extSrc @08:06:59.81 AS.reconfigure ASslow1 @08:06:59.89 AS.seek 0ml @08:08:40.73 AFV.select :carrierSolution @08:08:40.85 delay 2 seconds @08:08:42.89 AS.reconfigure ASconfig @08:08:42.95 AS.seek 0.5ml @08:09:12.01 delay 1 second @08:09:13.03 AFV.select :holdingCoil @08:09:13.15 ATV.dial :extSrc @08:09:13.17 AS.reconfigure ASslow1 @08:09:13.25 AS.seek 0ml @08:10:54.10 AFV.select :carrierSolution @08:10:54.22 delay 2 seconds @08:10:56.25 AS.reconfigure ASconfig @08:10:56.31 AS.seek 0.5ml @08:11:25.38 delay 1 second @08:11:26.40 AFV.select :holdingCoil @08:11:26.51 ATV.dial :extSrc @08:11:26.53 AS.reconfigure ASslow1 @08:11:26.61 AS.seek 0ml @08:13:07.45 AFV.select :carrierSolution @08:13:07.57 delay 2 seconds @08:13:09.60 AS.reconfigure ASconfig @08:13:09.66 AS.seek 0.5ml @08:13:38.71 delay 1 second @08:13:39.73 AFV.select :holdingCoil @08:13:39.84 ATV.dial :extSrc @08:13:39.86 AS.reconfigure ASslow1 @08:13:39.94 AS.seek 0ml @08:15:20.77 ATV.dial :air2 @08:15:22.13 AS.reconfigure ASslow0 @08:15:22.20 AS.seek 0.5ml @08:16:12.76 ATV.dial :extSrc @08:16:14.28 AS.seek 0.02ml @08:17:02.90 delay 1 second @08:17:03.92 ATV.dial :waste @08:17:05.35 AS.reconfigure ASconfig @08:17:05.41 AS.seek :empty @08:17:06.99 Session Ended @15:38:28.97CEST24-Apr-20
ESP2 v3.30 I2C Gateway v3.06 for core bus @15:38:31.62 Copyright 2018 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @15:38:32.21 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @15:38:38.98 Polling Can every 10:00 @15:38:40.81 SPE.coast @15:38:40.87 SPE.configure SPEconfig @15:38:40.96 AS.reconfigure ASconfig @15:38:41.01 AS.hold @15:38:41.06 AS.configure ASconfig @15:38:41.16 SS.reconfigure SSconfig @15:38:41.22 SS.hold @15:38:41.33 SS.configure SSconfig @15:38:41.43 CC.reconfigure CCconfig @15:38:41.47 CC.hold @15:38:41.53 CC.configure CCconfig @15:38:41.62 CS.reconfigure CSconfig @15:38:41.66 CS.hold @15:38:41.71 CS.configure CSconfig @15:38:42.18 PC.reconfigure PCconfig @15:38:42.23 PC.hold @15:38:42.31 PC.configure PCconfig @15:38:42.40 PS.configure PSconfig @15:38:42.52 Forearm.reconfigure LooseForearm @15:38:42.56 Forearm.hold @15:38:42.63 Forearm.configure LooseForearm @15:38:42.72 Elbow.reconfigure LooseElbow @15:38:42.77 Elbow.hold @15:38:42.84 Elbow.configure LooseElbow @15:38:42.95 SC.reconfigure SCloose @15:38:43.00 SC.hold @15:38:43.09 SC.configure SCloose @15:38:43.27 SE.reconfigure SEconfig @15:38:43.37 SE.hold @15:38:43.49 SE.configure SEconfig @15:38:43.57 CH.coast @15:38:43.69 CH.configure CHconfig @15:38:43.75 PH.coast @15:38:43.87 PH.configure PHconfig @15:38:43.93 PCR.off @15:38:43.97 PCR.pidtemp= 50,0.1,0,300,25,25 @15:38:44.01 PCR.caltemp= 2.8814,0.0372,0 @15:38:44.04 All dwarf controllers running firmware version 3.75 @15:38:44.23 Cleanly exited at 15:35:52CEST24-Apr-20 @15:38:44.26 Loading /home/esp/esp2/mission/stdMERsingleslugs2020.rb mission @15:38:45.10 AFV.select :carrierSolution @15:38:45.23 delay 2 seconds @15:38:45.52 Session Begun @15:38:47.28
AS.seek 0.5ml @15:39:16.31 delay 1 second @15:39:17.33 AFV.select :holdingCoil @15:39:17.44 ATV.dial :extSrc @15:39:17.93 AS.reconfigure ASslow1 @15:39:18.00 AS.seek 0.53ml @15:39:24.30 delay 5 seconds @15:39:29.40 AS.reconfigure ASslow2 @15:39:29.47 AS.seek 0.54ml @15:39:39.72 delay 20 seconds @15:39:59.81 AS.reconfigure ASslow1 @15:39:59.87 AS.seek 0.57ml @15:40:06.25 delay 5 seconds @15:40:11.34 AS.reconfigure ASslow2 @15:40:11.40 AS.seek 0.58ml @15:40:21.73 delay 20 seconds @15:40:41.82 AS.reconfigure ASslow1 @15:40:41.88 AS.seek 0.61ml @15:40:48.25 delay 5 seconds @15:40:53.35 AS.reconfigure ASslow2 @15:40:53.41 AS.seek 0.62ml @15:41:03.61 delay 20 seconds @15:41:23.70 AS.reconfigure ASslow1 @15:41:23.79 AS.seek 0.65ml @15:41:30.11 delay 5 seconds @15:41:35.21 AS.reconfigure ASslow2 @15:41:35.27 AS.seek 0.66ml @15:41:45.53 delay 20 seconds @15:42:05.57 AS.reconfigure ASslow1 @15:42:05.63 AS.seek 0.705ml @15:42:14.94 delay 10 seconds @15:42:24.97 ATV.dial :air1 @15:42:26.71 AS.seek 0.725ml @15:42:31.01 delay 3 seconds @15:42:34.03 ATV.dial :waste @15:42:34.60 AS.reconfigure ASslow0 @15:42:34.66 AS.seek :empty @15:43:48.14 Setting up PCR for ML -- writing /var/log/esp/ML20apr2415h.pcr @15:43:48.16 Building PCR Slug from extSrc with MM2 mix, PP4 primer @15:43:48.18 Priming with carrier @15:43:48.21 ATV.dial ARV @15:43:49.73 ARV.dial :carrier @15:43:50.12 AS.reconfigure ASnorm @15:43:50.19 AS.seek 0.499ml @15:44:15.75 AFV.select :carrierSolution @15:44:15.88 delay 2 seconds @15:44:17.92 AS.reconfigure ASconfig @15:44:17.98 AS.seek 1ml @15:44:47.06 delay 1 second @15:44:48.08 AFV.select :holdingCoil @15:44:48.20 delay 1 second @15:44:49.23 ATV.dial :waste @15:44:50.93 AS.reconfigure ASnorm @15:44:51.00 AS.seek :empty @15:45:41.75 AFV.select :carrierSolution @15:45:41.88 delay 2 seconds @15:45:43.92 AS.reconfigure ASconfig @15:45:43.98 AS.seek 0.5ml @15:46:13.12 delay 1 second @15:46:14.14 AFV.select :holdingCoil @15:46:14.27 Building Slug @15:46:14.28 ATV.dial :ARV @15:46:15.95 delay 2 seconds @15:46:17.98 ARV.dial :air1 @15:46:18.40 delay 2 seconds @15:46:20.49 AS.reconfigure ASslow2 @15:46:20.56 AS.seek 0.506ml @15:46:26.89 delay 2 seconds @15:46:28.91 ARV.dial :MM2 @15:46:30.42 delay 2 seconds @15:46:32.45 Adding MM2 @15:46:32.54 AS.seek 0.524ml @15:46:51.01 delay 25 seconds @15:47:16.04 ARV.dial :PP4 @15:47:16.46 Adding PP4 @15:47:16.47 delay 2 seconds @15:47:18.56 AS.seek 0.53ml @15:47:25.25 delay 10 seconds @15:47:35.29 ARV.dial :air3 @15:47:35.71 adding ARV air @15:47:35.72 delay 2 seconds @15:47:37.82 AS.seek 0.536ml @15:47:44.08 delay 5 seconds @15:47:49.11 ARV.dial :carrier @15:47:50.40 delay 7 seconds @15:47:57.49 AS.seek 0.594ml @15:48:56.12 delay 5 seconds @15:49:01.15 ATV.dial :extSrc @15:49:01.72 delay 2 seconds @15:49:03.81 AS.seek 0.6ml @15:49:10.15 delay 4 seconds @15:49:14.18 ATV.dial ARV @15:49:14.75 delay 2 seconds @15:49:16.78 ARV.dial :carrier @15:49:16.80 delay 2 seconds @15:49:18.89 AS.seek 0.65ml @15:50:09.76 delay 4 seconds @15:50:13.79 ATV.dial PCR @15:50:14.58 ASV.dial :waste1 @15:50:15.15 ARV.dial :air1 @15:50:15.57 Positioning Slug by 0.324ml @15:50:15.67 AS.seek 0.326ml @15:55:41.75 delay 5 seconds @15:55:46.78 ATV.dial ARV @15:55:47.58 ASV.dial :PCRlock @15:55:48.00 delay 5 seconds @15:55:53.04 Running ML Profile60C350 thermocycling @15:55:53.05 PCR.program Profile60C350 @15:55:53.19 Profile60C350 should complete in < 1:46:29 @15:55:53.21 PCR.run 2:18:06.25 @17:19:13.11 PCR.off @17:19:14.72 PCR for ML completed -- closed /var/log/esp/ML20apr2415h.pcr @17:19:14.81 Cleaning PCR @17:19:14.82 AFV.select :carrierSolution @17:19:14.95 delay 2 seconds @17:19:16.99 AS.reconfigure ASconfig @17:19:17.05 AS.seek 0.5ml @17:19:27.39 delay 1 second @17:19:28.41 AFV.select :holdingCoil @17:19:28.53 ASV.dial :waste1 @17:19:28.94 ATV.dial :air3 @17:19:30.27 AS.reconfigure ASslow1 @17:19:30.34 AS.seek 0.51ml @17:19:32.63 delay 2 seconds @17:19:34.65 ATV.dial :bleach @17:19:35.30 AS.seek 0.81ml @17:20:35.89 delay 3 seconds @17:20:38.92 ATV.dial :air3 @17:20:39.50 delay 3 seconds @17:20:42.59 AS.seek 0.835ml @17:20:47.90 delay 3 seconds @17:20:50.93 ATV.dial :bleach @17:20:51.58 AS.seek 0.82ml @17:20:54.92 delay 3 seconds @17:20:57.95 ARV.dial :waste @17:20:58.58 delay 3 seconds @17:21:01.61 ATV.dial :ARV @17:21:02.52 AS.reconfigure ASconfig @17:21:02.58 AS.seek 0.47ml @17:21:23.01 AFV.select :carrierSolution @17:21:23.13 delay 2 seconds @17:21:25.17 AS.seek 1ml @17:21:56.35 delay 1 second @17:21:57.37 AFV.select :holdingCoil @17:21:57.48 delay 1 second @17:21:58.52 AS.reconfigure ASfast @17:21:58.58 AS.seek 0ml @17:22:25.94 AFV.select :carrierSolution @17:22:26.07 delay 2 seconds @17:22:28.11 AS.reconfigure ASconfig @17:22:28.17 AS.seek 1ml @17:23:25.98 delay 1 second @17:23:27.00 AFV.select :holdingCoil @17:23:27.12 delay 1 second @17:23:28.16 AS.reconfigure ASfast @17:23:28.22 AS.seek 0ml @17:23:55.89 AFV.select :carrierSolution @17:23:56.02 delay 2 seconds @17:23:58.06 AS.reconfigure ASconfig @17:23:58.12 AS.seek 1ml @17:24:55.97 delay 1 second @17:24:56.99 AFV.select :holdingCoil @17:24:57.11 delay 1 second @17:24:58.15 AS.reconfigure ASfast @17:24:58.21 AS.seek 0ml @17:25:25.69 ARV.dial between carrier and air1 @17:25:26.20 ATV.dial :air3 @17:25:27.54 AS.reconfigure ASslow1 @17:25:27.61 AS.seek 0.01ml @17:25:30.03 delay 2 seconds @17:25:32.05 ATV.dial :bleach @17:25:32.71 AS.seek 0.81ml @17:28:13.87 delay 3 seconds @17:28:16.89 ATV.dial :air3 @17:28:17.47 delay 3 seconds @17:28:20.56 AS.seek 0.835ml @17:28:25.87 delay 3 seconds @17:28:28.90 ATV.dial :bleach @17:28:29.54 AS.seek 0.82ml @17:28:32.89 delay 3 seconds @17:28:35.92 ATV.dial PCR @17:28:36.35 AS.reconfigure ASconfig @17:28:36.41 AS.seek :empty @17:29:23.90 PCR.program :bleachProfile @17:29:23.97 PCR program should complete in < 2:45 @17:29:23.98 PCR.run 10:00 @17:32:19.76 PCR.off @17:32:19.80 AFV.select :carrierSolution @17:32:19.93 delay 2 seconds @17:32:21.97 AS.seek 1ml @17:33:19.86 delay 1 second @17:33:20.88 AFV.select :holdingCoil @17:33:20.99 delay 1 second @17:33:22.03 AS.reconfigure ASfast @17:33:22.09 AS.seek 0ml @17:33:49.47 delay 3 seconds @17:33:52.49 AFV.select :carrierSolution @17:33:52.61 delay 2 seconds @17:33:54.65 AS.reconfigure ASconfig @17:33:54.72 AS.seek 1ml @17:34:52.51 delay 1 second @17:34:53.53 AFV.select :holdingCoil @17:34:53.64 delay 1 second @17:34:54.68 AS.reconfigure ASfast @17:34:54.74 AS.seek 0ml @17:35:22.20 delay 3 seconds @17:35:25.22 AFV.select :carrierSolution @17:35:25.34 delay 2 seconds @17:35:27.38 AS.reconfigure ASconfig @17:35:27.45 AS.seek 1ml @17:36:25.23 delay 1 second @17:36:26.25 AFV.select :holdingCoil @17:36:26.36 delay 1 second @17:36:27.40 AS.reconfigure ASfast @17:36:27.46 AS.seek 0ml @17:36:54.81 delay 3 seconds @17:36:57.84 AFV.select :carrierSolution @17:36:57.96 delay 2 seconds @17:37:00.00 AS.reconfigure ASconfig @17:37:00.06 AS.seek 1ml @17:37:57.90 delay 1 second @17:37:58.92 AFV.select :holdingCoil @17:37:59.03 delay 1 second @17:38:00.07 AS.reconfigure ASfast @17:38:00.13 AS.seek 0ml @17:38:27.50 delay 3 seconds @17:38:30.52 AFV.select :carrierSolution @17:38:30.63 delay 2 seconds @17:38:32.67 AS.reconfigure ASconfig @17:38:32.74 AS.seek 1ml @17:39:30.50 delay 1 second @17:39:31.52 AFV.select :holdingCoil @17:39:31.63 delay 1 second @17:39:32.67 AS.reconfigure ASnorm @17:39:32.73 AS.seek 0ml @17:40:23.49
AFV.select :carrierSolution @17:40:23.61 delay 2 seconds @17:40:25.64 AS.reconfigure ASconfig @17:40:25.70 AS.seek 0.5ml @17:40:54.76 delay 1 second @17:40:55.78 AFV.select :holdingCoil @17:40:55.89 ATV.dial :air1 @17:40:57.43 AS.reconfigure ASslow2 @17:40:57.49 AS.seek 0.506ml @17:41:03.94 ATV.dial :extSrc @17:41:05.68 AS.seek 0.518ml @17:41:18.03 delay 5 seconds @17:41:23.06 ATV.dial :air1 @17:41:24.82 AS.reconfigure ASslow1 @17:41:24.88 AS.seek 0.548ml @17:41:31.19 ATV.dial :waste @17:41:31.77 AS.reconfigure ASconfig @17:41:31.83 AS.seek :empty @17:42:03.70 AFV.select :carrierSolution @17:42:03.82 delay 2 seconds @17:42:05.85 AS.seek 0.5ml @17:42:34.95 delay 1 second @17:42:35.97 AFV.select :holdingCoil @17:42:36.08 ATV.dial :air1 @17:42:36.72 AS.reconfigure ASslow2 @17:42:36.78 AS.seek 0.51ml @17:42:47.20 delay 5 seconds @17:42:52.22 ATV.dial :extSrc @17:42:53.96 AS.seek 0.53ml @17:43:14.45 delay 10 seconds @17:43:24.48 ATV.dial :air1 @17:43:26.22 AS.reconfigure ASslow1 @17:43:26.28 AS.seek 0.56ml @17:43:32.56 ATV.dial :waste @17:43:33.13 AS.reconfigure ASconfig @17:43:33.19 AS.seek :empty @17:44:05.93 Setting up PCR for ML -- writing /var/log/esp/ML20apr2417h.pcr @17:44:05.95 Building PCR Slug from extSrc with MM2 mix, PP4 primer @17:44:05.98 Priming with carrier @17:44:06.01 ATV.dial ARV @17:44:07.51 ARV.dial :carrier @17:44:07.86 AS.reconfigure ASnorm @17:44:07.94 AS.seek 0.499ml @17:44:33.53 AFV.select :carrierSolution @17:44:33.66 delay 2 seconds @17:44:35.70 AS.reconfigure ASconfig @17:44:35.86 AS.seek 1ml @17:45:04.95 delay 1 second @17:45:05.97 AFV.select :holdingCoil @17:45:06.09 delay 1 second @17:45:07.12 ATV.dial :waste @17:45:08.82 AS.reconfigure ASnorm @17:45:08.89 AS.seek :empty @17:45:59.65 AFV.select :carrierSolution @17:45:59.78 delay 2 seconds @17:46:01.82 AS.reconfigure ASconfig @17:46:01.88 AS.seek 0.5ml @17:46:30.97 delay 1 second @17:46:31.99 AFV.select :holdingCoil @17:46:32.11 Building Slug @17:46:32.13 ATV.dial :ARV @17:46:33.79 delay 2 seconds @17:46:35.82 ARV.dial :air1 @17:46:36.22 delay 2 seconds @17:46:38.31 AS.reconfigure ASslow2 @17:46:38.38 AS.seek 0.506ml @17:46:44.86 delay 2 seconds @17:46:46.88 ARV.dial :MM2 @17:46:48.39 delay 2 seconds @17:46:50.42 Adding MM2 @17:46:50.52 AS.seek 0.524ml @17:47:09.11 delay 25 seconds @17:47:34.14 ARV.dial :PP4 @17:47:34.54 Adding PP4 @17:47:34.56 delay 2 seconds @17:47:36.65 AS.seek 0.53ml @17:47:43.03 delay 10 seconds @17:47:53.06 ARV.dial :air3 @17:47:53.47 adding ARV air @17:47:53.49 delay 2 seconds @17:47:55.58 AS.seek 0.536ml @17:48:01.86 delay 5 seconds @17:48:06.89 ARV.dial :carrier @17:48:08.17 delay 7 seconds @17:48:15.26 AS.seek 0.594ml @17:49:13.86 delay 5 seconds @17:49:18.89 ATV.dial :extSrc @17:49:19.47 delay 2 seconds @17:49:21.56 AS.seek 0.6ml @17:49:28.03 delay 4 seconds @17:49:32.06 ATV.dial ARV @17:49:32.62 delay 2 seconds @17:49:34.65 ARV.dial :carrier @17:49:34.67 delay 2 seconds @17:49:36.76 AS.seek 0.65ml @17:50:27.40 delay 4 seconds @17:50:31.43 ATV.dial PCR @17:50:32.22 ASV.dial :waste1 @17:50:32.24 ARV.dial :air1 @17:50:32.68 Positioning Slug by 0.324ml @17:50:32.79 AS.seek 0.326ml @17:55:58.75 delay 5 seconds @17:56:03.78 ATV.dial ARV @17:56:04.58 ASV.dial :PCRlock @17:56:05.03 delay 5 seconds @17:56:10.06 Running ML Profile60C350 thermocycling @17:56:10.08 PCR.program Profile60C350 @17:56:10.22 Profile60C350 should complete in < 1:46:29 @17:56:10.24 PCR.run 2:18:06.25 @19:19:36.10 PCR.off @19:19:37.74 PCR for ML completed -- closed /var/log/esp/ML20apr2417h.pcr @19:19:37.82 Cleaning PCR @19:19:37.84 AFV.select :carrierSolution @19:19:37.97 delay 2 seconds @19:19:40.01 AS.reconfigure ASconfig @19:19:40.07 AS.seek 0.5ml @19:19:50.39 delay 1 second @19:19:51.41 AFV.select :holdingCoil @19:19:51.52 ASV.dial :waste1 @19:19:51.93 ATV.dial :air3 @19:19:53.25 AS.reconfigure ASslow1 @19:19:53.32 AS.seek 0.51ml @19:19:55.62 delay 2 seconds @19:19:57.64 ATV.dial :bleach @19:19:58.29 AS.seek 0.81ml @19:20:58.87 delay 3 seconds @19:21:01.89 ATV.dial :air3 @19:21:02.47 delay 3 seconds @19:21:05.56 AS.seek 0.835ml @19:21:10.87 delay 3 seconds @19:21:13.90 ATV.dial :bleach @19:21:14.55 AS.seek 0.82ml @19:21:17.89 delay 3 seconds @19:21:20.91 ARV.dial :waste @19:21:21.54 delay 3 seconds @19:21:24.57 ATV.dial :ARV @19:21:25.49 AS.reconfigure ASconfig @19:21:25.55 AS.seek 0.47ml @19:21:45.98 AFV.select :carrierSolution @19:21:46.11 delay 2 seconds @19:21:48.15 AS.seek 1ml @19:22:18.90 delay 1 second @19:22:19.92 AFV.select :holdingCoil @19:22:20.03 delay 1 second @19:22:21.07 AS.reconfigure ASfast @19:22:21.13 AS.seek 0ml @19:22:48.75 AFV.select :carrierSolution @19:22:48.87 delay 2 seconds @19:22:50.91 AS.reconfigure ASconfig @19:22:50.97 AS.seek 1ml @19:23:48.75 delay 1 second @19:23:49.77 AFV.select :holdingCoil @19:23:49.88 delay 1 second @19:23:50.92 AS.reconfigure ASfast @19:23:50.98 AS.seek 0ml @19:24:18.34 AFV.select :carrierSolution @19:24:18.46 delay 2 seconds @19:24:20.50 AS.reconfigure ASconfig @19:24:20.56 AS.seek 1ml @19:25:18.34 delay 1 second @19:25:19.36 AFV.select :holdingCoil @19:25:19.47 delay 1 second @19:25:20.51 AS.reconfigure ASfast @19:25:20.57 AS.seek 0ml @19:25:47.93 ARV.dial between carrier and air1 @19:25:48.43 ATV.dial :air3 @19:25:49.75 AS.reconfigure ASslow1 @19:25:49.82 AS.seek 0.01ml @19:25:52.23 delay 2 seconds @19:25:54.25 ATV.dial :bleach @19:25:54.91 AS.seek 0.81ml @19:28:36.08 delay 3 seconds @19:28:39.10 ATV.dial :air3 @19:28:39.68 delay 3 seconds @19:28:42.78 AS.seek 0.835ml @19:28:48.09 delay 3 seconds @19:28:51.11 ATV.dial :bleach @19:28:51.77 AS.seek 0.82ml @19:28:55.04 delay 3 seconds @19:28:58.07 ATV.dial PCR @19:28:58.49 AS.reconfigure ASconfig @19:28:58.55 AS.seek :empty @19:29:46.04 PCR.program :bleachProfile @19:29:46.11 PCR program should complete in < 2:45 @19:29:46.12 PCR.run 10:00 @19:32:41.90 PCR.off @19:32:41.94 AFV.select :carrierSolution @19:32:42.06 delay 2 seconds @19:32:44.10 AS.seek 1ml @19:33:41.93 delay 1 second @19:33:42.95 AFV.select :holdingCoil @19:33:43.06 delay 1 second @19:33:44.10 AS.reconfigure ASfast @19:33:44.16 AS.seek 0ml @19:34:11.53 delay 3 seconds @19:34:14.55 AFV.select :carrierSolution @19:34:14.67 delay 2 seconds @19:34:16.71 AS.reconfigure ASconfig @19:34:16.78 AS.seek 1ml @19:35:14.59 delay 1 second @19:35:15.61 AFV.select :holdingCoil @19:35:15.72 delay 1 second @19:35:16.76 AS.reconfigure ASfast @19:35:16.82 AS.seek 0ml @19:35:44.18 delay 3 seconds @19:35:47.21 AFV.select :carrierSolution @19:35:47.32 delay 2 seconds @19:35:49.36 AS.reconfigure ASconfig @19:35:49.43 AS.seek 1ml @19:36:47.20 delay 1 second @19:36:48.22 AFV.select :holdingCoil @19:36:48.33 delay 1 second @19:36:49.37 AS.reconfigure ASfast @19:36:49.43 AS.seek 0ml @19:37:16.76 delay 3 seconds @19:37:19.79 AFV.select :carrierSolution @19:37:19.91 delay 2 seconds @19:37:21.95 AS.reconfigure ASconfig @19:37:22.01 AS.seek 1ml @19:38:19.78 delay 1 second @19:38:20.80 AFV.select :holdingCoil @19:38:20.91 delay 1 second @19:38:21.95 AS.reconfigure ASfast @19:38:22.01 AS.seek 0ml @19:38:49.37 delay 3 seconds @19:38:52.86 AFV.select :carrierSolution @19:38:52.98 delay 2 seconds @19:38:55.02 AS.reconfigure ASconfig @19:38:55.08 AS.seek 1ml @19:39:52.89 delay 1 second @19:39:53.91 AFV.select :holdingCoil @19:39:54.03 delay 1 second @19:39:55.07 AS.reconfigure ASnorm @19:39:55.13 AS.seek 0ml @19:40:45.91
AFV.select :carrierSolution @19:40:46.03 delay 2 seconds @19:40:48.07 AS.reconfigure ASconfig @19:40:48.13 AS.seek 0.5ml @19:41:17.18 delay 1 second @19:41:18.20 AFV.select :holdingCoil @19:41:18.31 ATV.dial :air1 @19:41:19.83 AS.reconfigure ASslow2 @19:41:19.90 AS.seek 0.506ml @19:41:26.18 ATV.dial :extSrc @19:41:27.91 AS.seek 0.518ml @19:41:40.18 delay 5 seconds @19:41:45.21 ATV.dial :air1 @19:41:46.97 AS.reconfigure ASslow1 @19:41:47.04 AS.seek 0.548ml @19:41:53.42 ATV.dial :waste @19:41:54.01 AS.reconfigure ASconfig @19:41:54.07 AS.seek :empty @19:42:25.93 AFV.select :carrierSolution @19:42:26.05 delay 2 seconds @19:42:28.08 AS.seek 0.5ml @19:42:57.20 delay 1 second @19:42:58.22 AFV.select :holdingCoil @19:42:58.33 ATV.dial :air1 @19:42:58.97 AS.reconfigure ASslow1 @19:42:59.03 AS.seek 0.52ml @19:43:03.40 delay 5 seconds @19:43:08.43 ATV.dial :extSrc @19:43:10.16 AS.reconfigure ASslow2 @19:43:10.22 AS.seek 0.545ml @19:43:35.57 delay 10 seconds @19:43:45.60 ATV.dial :air1 @19:43:47.33 AS.reconfigure ASslow1 @19:43:47.39 AS.seek 0.575ml @19:43:53.70 ATV.dial :waste @19:43:54.27 AS.reconfigure ASconfig @19:43:54.33 AS.seek :empty @19:44:27.92 Setting up PCR for ML -- writing /var/log/esp/ML20apr2419h.pcr @19:44:27.95 Building PCR Slug from extSrc with MM2 mix, PP4 primer @19:44:27.98 Priming with carrier @19:44:28.00 ATV.dial ARV @19:44:29.50 ARV.dial :carrier @19:44:29.88 AS.reconfigure ASnorm @19:44:29.95 AS.seek 0.499ml @19:44:55.53 AFV.select :carrierSolution @19:44:55.66 delay 2 seconds @19:44:57.70 AS.reconfigure ASconfig @19:44:57.77 AS.seek 1ml @19:45:26.87 delay 1 second @19:45:27.89 AFV.select :holdingCoil @19:45:28.01 delay 1 second @19:45:29.04 ATV.dial :waste @19:45:30.74 AS.reconfigure ASnorm @19:45:30.81 AS.seek :empty @19:46:21.58 AFV.select :carrierSolution @19:46:21.70 delay 2 seconds @19:46:23.74 AS.reconfigure ASconfig @19:46:23.80 AS.seek 0.5ml @19:46:52.93 delay 1 second @19:46:53.95 AFV.select :holdingCoil @19:46:54.08 Building Slug @19:46:54.09 ATV.dial :ARV @19:46:55.76 delay 2 seconds @19:46:57.79 ARV.dial :air1 @19:46:58.20 delay 2 seconds @19:47:00.29 AS.reconfigure ASslow2 @19:47:00.36 AS.seek 0.506ml @19:47:06.89 delay 2 seconds @19:47:08.91 ARV.dial :MM2 @19:47:10.42 delay 2 seconds @19:47:12.45 Adding MM2 @19:47:12.54 AS.seek 0.524ml @19:47:31.04 delay 25 seconds @19:47:56.07 ARV.dial :PP4 @19:47:56.48 Adding PP4 @19:47:56.50 delay 2 seconds @19:47:58.59 AS.seek 0.53ml @19:48:05.17 delay 10 seconds @19:48:15.20 ARV.dial :air3 @19:48:15.61 adding ARV air @19:48:15.63 delay 2 seconds @19:48:17.72 AS.seek 0.536ml @19:48:24.03 delay 5 seconds @19:48:29.06 ARV.dial :carrier @19:48:30.34 delay 7 seconds @19:48:37.43 AS.seek 0.594ml @19:49:35.92 delay 5 seconds @19:49:40.95 ATV.dial :extSrc @19:49:41.53 delay 2 seconds @19:49:43.62 AS.seek 0.6ml @19:49:50.32 delay 4 seconds @19:49:54.35 ATV.dial ARV @19:49:54.92 delay 2 seconds @19:49:56.95 ARV.dial :carrier @19:49:56.97 delay 2 seconds @19:49:59.07 AS.seek 0.65ml @19:50:49.72 delay 4 seconds @19:50:53.75 ATV.dial PCR @19:50:54.54 ASV.dial :waste1 @19:50:54.56 ARV.dial :air1 @19:50:55.00 Positioning Slug by 0.324ml @19:50:55.10 AS.seek 0.326ml @19:56:21.17 delay 5 seconds @19:56:26.20 ATV.dial ARV @19:56:27.00 ASV.dial :PCRlock @19:56:27.42 delay 5 seconds @19:56:32.45 Running ML Profile60C350 thermocycling @19:56:32.47 PCR.program Profile60C350 @19:56:32.60 Profile60C350 should complete in < 1:46:29 @19:56:32.62 PCR.run 2:18:06.25 @21:19:55.88 PCR.off @21:19:57.52 PCR for ML completed -- closed /var/log/esp/ML20apr2419h.pcr @21:19:57.61 Cleaning PCR @21:19:57.62 AFV.select :carrierSolution @21:19:57.75 delay 2 seconds @21:19:59.79 AS.reconfigure ASconfig @21:19:59.85 AS.seek 0.5ml @21:20:10.17 delay 1 second @21:20:11.19 AFV.select :holdingCoil @21:20:11.30 ASV.dial :waste1 @21:20:11.72 ATV.dial :air3 @21:20:13.05 AS.reconfigure ASslow1 @21:20:13.12 AS.seek 0.51ml @21:20:15.41 delay 2 seconds @21:20:17.88 ATV.dial :bleach @21:20:18.54 AS.seek 0.81ml @21:21:19.14 delay 3 seconds @21:21:22.17 ATV.dial :air3 @21:21:22.75 delay 3 seconds @21:21:25.84 AS.seek 0.835ml @21:21:31.17 delay 3 seconds @21:21:34.20 ATV.dial :bleach @21:21:34.85 AS.seek 0.82ml @21:21:38.20 delay 3 seconds @21:21:41.23 ARV.dial :waste @21:21:41.86 delay 3 seconds @21:21:44.89 ATV.dial :ARV @21:21:45.82 AS.reconfigure ASconfig @21:21:45.89 AS.seek 0.47ml @21:22:06.31 AFV.select :carrierSolution @21:22:06.44 delay 2 seconds @21:22:08.48 AS.seek 1ml @21:22:39.25 delay 1 second @21:22:40.27 AFV.select :holdingCoil @21:22:40.39 delay 1 second @21:22:41.43 AS.reconfigure ASfast @21:22:41.49 AS.seek 0ml @21:23:08.87 AFV.select :carrierSolution @21:23:09.00 delay 2 seconds @21:23:11.04 AS.reconfigure ASconfig @21:23:11.10 AS.seek 1ml @21:24:08.92 delay 1 second @21:24:09.94 AFV.select :holdingCoil @21:24:10.06 delay 1 second @21:24:11.10 AS.reconfigure ASfast @21:24:11.16 AS.seek 0ml @21:24:38.53 AFV.select :carrierSolution @21:24:38.66 delay 2 seconds @21:24:40.70 AS.reconfigure ASconfig @21:24:40.76 AS.seek 1ml @21:25:38.54 delay 1 second @21:25:39.56 AFV.select :holdingCoil @21:25:39.67 delay 1 second @21:25:40.71 AS.reconfigure ASfast @21:25:40.77 AS.seek 0ml @21:26:08.32 ARV.dial between carrier and air1 @21:26:08.82 ATV.dial :air3 @21:26:10.14 AS.reconfigure ASslow1 @21:26:10.21 AS.seek 0.01ml @21:26:12.57 delay 2 seconds @21:26:14.59 ATV.dial :bleach @21:26:15.24 AS.seek 0.81ml @21:28:56.40 delay 3 seconds @21:28:59.43 ATV.dial :air3 @21:29:00.00 delay 3 seconds @21:29:03.09 AS.seek 0.835ml @21:29:08.40 delay 3 seconds @21:29:11.43 ATV.dial :bleach @21:29:12.08 AS.seek 0.82ml @21:29:15.39 delay 3 seconds @21:29:18.42 ATV.dial PCR @21:29:18.83 AS.reconfigure ASconfig @21:29:18.90 AS.seek :empty @21:30:06.39 PCR.program :bleachProfile @21:30:06.46 PCR program should complete in < 2:45 @21:30:06.47 PCR.run 10:00 @21:33:02.25 PCR.off @21:33:02.29 AFV.select :carrierSolution @21:33:02.43 delay 2 seconds @21:33:04.46 AS.seek 1ml @21:34:02.31 delay 1 second @21:34:03.33 AFV.select :holdingCoil @21:34:03.44 delay 1 second @21:34:04.48 AS.reconfigure ASfast @21:34:04.54 AS.seek 0ml @21:34:32.03 delay 3 seconds @21:34:35.06 AFV.select :carrierSolution @21:34:35.18 delay 2 seconds @21:34:37.22 AS.reconfigure ASconfig @21:34:37.29 AS.seek 1ml @21:35:35.06 delay 1 second @21:35:36.08 AFV.select :holdingCoil @21:35:36.20 delay 1 second @21:35:37.24 AS.reconfigure ASfast @21:35:37.30 AS.seek 0ml @21:36:04.68 delay 3 seconds @21:36:07.71 AFV.select :carrierSolution @21:36:07.83 delay 2 seconds @21:36:09.87 AS.reconfigure ASconfig @21:36:09.94 AS.seek 1ml @21:37:07.71 delay 1 second @21:37:08.74 AFV.select :holdingCoil @21:37:08.86 delay 1 second @21:37:09.90 AS.reconfigure ASfast @21:37:09.96 AS.seek 0ml @21:37:37.32 delay 3 seconds @21:37:40.35 AFV.select :carrierSolution @21:37:40.47 delay 2 seconds @21:37:42.51 AS.reconfigure ASconfig @21:37:42.58 AS.seek 1ml @21:38:40.34 delay 1 second @21:38:41.36 AFV.select :holdingCoil @21:38:41.48 delay 1 second @21:38:42.95 AS.reconfigure ASfast @21:38:43.04 AS.seek 0ml @21:39:10.37 delay 3 seconds @21:39:13.40 AFV.select :carrierSolution @21:39:13.52 delay 2 seconds @21:39:15.56 AS.reconfigure ASconfig @21:39:15.63 AS.seek 1ml @21:40:13.39 delay 1 second @21:40:14.41 AFV.select :holdingCoil @21:40:14.52 delay 1 second @21:40:15.56 AS.reconfigure ASnorm @21:40:15.62 AS.seek 0ml @21:41:06.38
AFV.select :carrierSolution @21:41:06.50 delay 2 seconds @21:41:08.53 AS.reconfigure ASconfig @21:41:08.59 AS.seek 0.5ml @21:41:37.64 delay 1 second @21:41:38.66 AFV.select :holdingCoil @21:41:38.77 ATV.dial :air1 @21:41:40.30 AS.reconfigure ASslow2 @21:41:40.36 AS.seek 0.506ml @21:41:46.70 ATV.dial :extSrc @21:41:48.54 AS.seek 0.518ml @21:42:01.15 delay 5 seconds @21:42:06.18 ATV.dial :air1 @21:42:07.93 AS.reconfigure ASslow1 @21:42:07.99 AS.seek 0.548ml @21:42:14.29 ATV.dial :waste @21:42:14.87 AS.reconfigure ASconfig @21:42:14.93 AS.seek :empty @21:42:46.79 AFV.select :carrierSolution @21:42:46.91 delay 2 seconds @21:42:48.94 AS.seek 0.5ml @21:43:18.06 delay 1 second @21:43:19.08 AFV.select :holdingCoil @21:43:19.19 ATV.dial :air1 @21:43:19.83 AS.reconfigure ASslow1 @21:43:19.89 AS.seek 0.52ml @21:43:24.23 delay 5 seconds @21:43:29.26 ATV.dial :extSrc @21:43:30.99 AS.reconfigure ASslow2 @21:43:31.05 AS.seek 0.538ml @21:43:49.36 delay 10 seconds @21:43:59.39 ATV.dial :air1 @21:44:01.14 AS.reconfigure ASslow1 @21:44:01.21 AS.seek 0.568ml @21:44:07.50 ATV.dial :waste @21:44:08.08 AS.reconfigure ASconfig @21:44:08.14 AS.seek :empty @21:44:41.34 Setting up PCR for NM -- writing /var/log/esp/NM20apr2421h.pcr @21:44:41.37 Building PCR Slug from extSrc with MM1 mix, PP1 primer @21:44:41.39 Priming with carrier @21:44:41.42 ATV.dial ARV @21:44:42.92 ARV.dial :carrier @21:44:43.29 AS.reconfigure ASnorm @21:44:43.36 AS.seek 0.499ml @21:45:08.98 AFV.select :carrierSolution @21:45:09.10 delay 2 seconds @21:45:11.14 AS.reconfigure ASconfig @21:45:11.21 AS.seek 1ml @21:45:40.28 delay 1 second @21:45:41.30 AFV.select :holdingCoil @21:45:41.42 delay 1 second @21:45:42.45 ATV.dial :waste @21:45:44.15 AS.reconfigure ASnorm @21:45:44.22 AS.seek :empty @21:46:34.98 AFV.select :carrierSolution @21:46:35.11 delay 2 seconds @21:46:37.15 AS.reconfigure ASconfig @21:46:37.21 AS.seek 0.5ml @21:47:06.32 delay 1 second @21:47:07.34 AFV.select :holdingCoil @21:47:07.46 Building Slug @21:47:07.48 ATV.dial :ARV @21:47:09.15 delay 2 seconds @21:47:11.18 ARV.dial :air1 @21:47:11.58 delay 2 seconds @21:47:13.67 AS.reconfigure ASslow2 @21:47:13.74 AS.seek 0.506ml @21:47:19.90 delay 2 seconds @21:47:21.92 ARV.dial :MM1 @21:47:22.54 delay 2 seconds @21:47:24.57 Adding MM1 @21:47:24.67 AS.seek 0.524ml @21:47:42.92 delay 25 seconds @21:48:07.95 ARV.dial :PP1 @21:48:08.36 Adding PP1 @21:48:08.38 delay 2 seconds @21:48:10.47 AS.seek 0.53ml @21:48:16.75 delay 10 seconds @21:48:26.78 ARV.dial :air1 @21:48:27.19 adding ARV air @21:48:27.20 delay 2 seconds @21:48:29.30 AS.seek 0.536ml @21:48:35.59 delay 5 seconds @21:48:40.61 ARV.dial :carrier @21:48:41.03 delay 7 seconds @21:48:48.12 AS.seek 0.594ml @21:49:46.70 delay 5 seconds @21:49:51.73 ATV.dial :extSrc @21:49:52.76 delay 2 seconds @21:49:54.85 AS.seek 0.6ml @21:50:01.23 delay 4 seconds @21:50:05.26 ATV.dial ARV @21:50:05.82 delay 2 seconds @21:50:07.85 ARV.dial :carrier @21:50:07.87 delay 2 seconds @21:50:09.96 AS.seek 0.65ml @21:51:00.45 delay 4 seconds @21:51:04.48 ATV.dial PCR @21:51:05.26 ASV.dial :waste1 @21:51:05.28 ARV.dial :air1 @21:51:05.72 Positioning Slug by 0.324ml @21:51:05.83 AS.seek 0.326ml @21:56:32.25 delay 5 seconds @21:56:37.28 ATV.dial ARV @21:56:38.07 ASV.dial :PCRlock @21:56:38.50 delay 5 seconds @21:56:43.53 Running NM Profile60C300 thermocycling @21:56:43.55 PCR.program Profile60C300 @21:56:43.67 Profile60C300 should complete in < 1:40:53 @21:56:43.69 PCR.run 2:11:06.25 @23:18:48.37 PCR.off @23:18:48.98 PCR for NM completed -- closed /var/log/esp/NM20apr2421h.pcr @23:18:49.07 Cleaning PCR @23:18:49.08 AFV.select :carrierSolution @23:18:49.21 delay 2 seconds @23:18:51.25 AS.reconfigure ASconfig @23:18:51.31 AS.seek 0.5ml @23:19:01.64 delay 1 second @23:19:02.66 AFV.select :holdingCoil @23:19:02.77 ASV.dial :waste1 @23:19:03.18 ATV.dial :air3 @23:19:04.50 AS.reconfigure ASslow1 @23:19:04.57 AS.seek 0.51ml @23:19:06.87 delay 2 seconds @23:19:08.89 ATV.dial :bleach @23:19:09.55 AS.seek 0.81ml @23:20:10.15 delay 3 seconds @23:20:13.17 ATV.dial :air3 @23:20:13.75 delay 3 seconds @23:20:16.84 AS.seek 0.835ml @23:20:22.15 delay 3 seconds @23:20:25.17 ATV.dial :bleach @23:20:25.82 AS.seek 0.82ml @23:20:29.14 delay 3 seconds @23:20:32.16 ARV.dial :waste @23:20:32.79 delay 3 seconds @23:20:35.82 ATV.dial :ARV @23:20:36.74 AS.reconfigure ASconfig @23:20:36.80 AS.seek 0.47ml @23:20:57.69 AFV.select :carrierSolution @23:20:57.81 delay 2 seconds @23:20:59.85 AS.seek 1ml @23:21:30.68 delay 1 second @23:21:31.70 AFV.select :holdingCoil @23:21:31.82 delay 1 second @23:21:32.86 AS.reconfigure ASfast @23:21:32.92 AS.seek 0ml @23:22:00.40 AFV.select :carrierSolution @23:22:00.53 delay 2 seconds @23:22:02.57 AS.reconfigure ASconfig @23:22:02.63 AS.seek 1ml @23:23:00.45 delay 1 second @23:23:01.47 AFV.select :holdingCoil @23:23:01.59 delay 1 second @23:23:02.63 AS.reconfigure ASfast @23:23:02.69 AS.seek 0ml @23:23:30.04 AFV.select :carrierSolution @23:23:30.17 delay 2 seconds @23:23:32.21 AS.reconfigure ASconfig @23:23:32.27 AS.seek 1ml @23:24:30.03 delay 1 second @23:24:31.05 AFV.select :holdingCoil @23:24:31.17 delay 1 second @23:24:32.21 AS.reconfigure ASfast @23:24:32.27 AS.seek 0ml @23:24:59.64 ARV.dial between carrier and air1 @23:25:00.15 ATV.dial :air3 @23:25:01.49 AS.reconfigure ASslow1 @23:25:01.56 AS.seek 0.01ml @23:25:03.87 delay 2 seconds @23:25:05.89 ATV.dial :bleach @23:25:06.55 AS.seek 0.81ml @23:27:47.68 delay 3 seconds @23:27:50.71 ATV.dial :air3 @23:27:51.29 delay 3 seconds @23:27:54.38 AS.seek 0.835ml @23:27:59.71 delay 3 seconds @23:28:02.74 ATV.dial :bleach @23:28:03.39 AS.seek 0.82ml @23:28:06.87 delay 3 seconds @23:28:09.90 ATV.dial PCR @23:28:10.32 AS.reconfigure ASconfig @23:28:10.38 AS.seek :empty @23:28:57.86 PCR.program :bleachProfile @23:28:57.92 PCR program should complete in < 2:45 @23:28:57.94 PCR.run 10:00 @23:31:53.71 PCR.off @23:31:53.75 AFV.select :carrierSolution @23:31:53.87 delay 2 seconds @23:31:55.91 AS.seek 1ml @23:32:53.76 delay 1 second @23:32:54.78 AFV.select :holdingCoil @23:32:54.89 delay 1 second @23:32:55.93 AS.reconfigure ASfast @23:32:55.99 AS.seek 0ml @23:33:23.36 delay 3 seconds @23:33:26.39 AFV.select :carrierSolution @23:33:26.51 delay 2 seconds @23:33:28.55 AS.reconfigure ASconfig @23:33:28.62 AS.seek 1ml @23:34:26.40 delay 1 second @23:34:27.42 AFV.select :holdingCoil @23:34:27.54 delay 1 second @23:34:28.59 AS.reconfigure ASfast @23:34:28.65 AS.seek 0ml @23:34:56.03 delay 3 seconds @23:34:59.06 AFV.select :carrierSolution @23:34:59.18 delay 2 seconds @23:35:01.22 AS.reconfigure ASconfig @23:35:01.29 AS.seek 1ml @23:35:59.14 delay 1 second @23:36:00.16 AFV.select :holdingCoil @23:36:00.27 delay 1 second @23:36:01.31 AS.reconfigure ASfast @23:36:01.37 AS.seek 0ml @23:36:28.75 delay 3 seconds @23:36:31.78 AFV.select :carrierSolution @23:36:31.90 delay 2 seconds @23:36:33.94 AS.reconfigure ASconfig @23:36:34.01 AS.seek 1ml @23:37:31.86 delay 1 second @23:37:32.88 AFV.select :holdingCoil @23:37:32.99 delay 1 second @23:37:34.03 AS.reconfigure ASfast @23:37:34.09 AS.seek 0ml @23:38:01.46 delay 3 seconds @23:38:04.49 AFV.select :carrierSolution @23:38:04.61 delay 2 seconds @23:38:06.65 AS.reconfigure ASconfig @23:38:06.73 AS.seek 1ml @23:39:04.51 delay 1 second @23:39:05.53 AFV.select :holdingCoil @23:39:05.64 delay 1 second @23:39:06.68 AS.reconfigure ASnorm @23:39:06.74 AS.seek 0ml @23:39:57.45
AFV.select :carrierSolution @23:39:57.56 delay 2 seconds @23:39:59.59 AS.reconfigure ASconfig @23:39:59.65 AS.seek 0.5ml @23:40:28.73 delay 1 second @23:40:29.75 AFV.select :holdingCoil @23:40:29.86 ATV.dial :air1 @23:40:31.39 AS.reconfigure ASslow2 @23:40:31.45 AS.seek 0.506ml @23:40:37.75 ATV.dial :extSrc @23:40:40.02 AS.seek 0.518ml @23:40:52.50 delay 5 seconds @23:40:57.53 ATV.dial :air1 @23:40:59.27 AS.reconfigure ASslow1 @23:40:59.33 AS.seek 0.548ml @23:41:05.79 ATV.dial :waste @23:41:06.37 AS.reconfigure ASconfig @23:41:06.43 AS.seek :empty @23:41:38.29 deconEluateRecoveryLine extSrc @23:41:38.30 ATV.dial :waste @23:41:38.34 AS.reconfigure ASnorm @23:41:38.41 AS.seek 0ml @23:41:38.82 ATV.dial :air3 @23:41:39.52 AS.reconfigure ASslow1 @23:41:39.59 AS.seek 0.01ml @23:41:41.93 delay 2 seconds @23:41:43.95 ATV.dial :bleach @23:41:44.48 AS.seek 0.31ml @23:42:45.14 delay 3 seconds @23:42:48.16 ATV.dial :air3 @23:42:48.75 delay 3 seconds @23:42:51.84 AS.seek 0.335ml @23:42:57.17 delay 3 seconds @23:43:00.19 ATV.dial :bleach @23:43:00.83 AS.seek 0.32ml @23:43:04.15 delay 3 seconds @23:43:07.17 ATV.dial :extSrc @23:43:07.80 AS.seek 0ml @23:44:12.43 AFV.select :carrierSolution @23:44:12.55 delay 2 seconds @23:44:14.58 AS.reconfigure ASconfig @23:44:14.64 AS.seek 0.5ml @23:44:43.70 delay 1 second @23:44:44.72 AFV.select :holdingCoil @23:44:44.84 ATV.dial :extSrc @23:44:44.86 AS.reconfigure ASslow1 @23:44:44.94 AS.seek 0ml @23:46:25.87 AFV.select :carrierSolution @23:46:25.99 delay 2 seconds @23:46:28.02 AS.reconfigure ASconfig @23:46:28.08 AS.seek 0.5ml @23:46:57.11 delay 1 second @23:46:58.13 AFV.select :holdingCoil @23:46:58.24 ATV.dial :extSrc @23:46:58.26 AS.reconfigure ASslow1 @23:46:58.34 AS.seek 0ml @23:48:39.18 AFV.select :carrierSolution @23:48:39.30 delay 2 seconds @23:48:41.33 AS.reconfigure ASconfig @23:48:41.39 AS.seek 0.5ml @23:49:10.43 delay 1 second @23:49:11.45 AFV.select :holdingCoil @23:49:11.57 ATV.dial :extSrc @23:49:11.59 AS.reconfigure ASslow1 @23:49:11.67 AS.seek 0ml @23:50:52.50 AFV.select :carrierSolution @23:50:52.62 delay 2 seconds @23:50:54.65 AS.reconfigure ASconfig @23:50:54.71 AS.seek 0.5ml @23:51:23.76 delay 1 second @23:51:24.78 AFV.select :holdingCoil @23:51:24.90 ATV.dial :extSrc @23:51:24.92 AS.reconfigure ASslow1 @23:51:25.00 AS.seek 0ml @23:53:05.84 ATV.dial :air2 @23:53:07.19 AS.reconfigure ASslow0 @23:53:07.25 AS.seek 0.5ml @23:53:57.84 ATV.dial :extSrc @23:53:59.35 AS.seek 0.02ml @23:54:47.96 delay 1 second @23:54:48.98 ATV.dial :waste @23:54:50.29 AS.reconfigure ASconfig @23:54:50.35 AS.seek :empty @23:54:51.87 deconEluateRecoveryLine extSrc @23:54:51.88 ATV.dial :waste @23:54:51.92 AS.reconfigure ASnorm @23:54:51.99 AS.seek 0ml @23:54:52.39 ATV.dial :air3 @23:54:53.25 AS.reconfigure ASslow1 @23:54:53.32 AS.seek 0.01ml @23:54:55.68 delay 2 seconds @23:54:57.70 ATV.dial :bleach @23:54:58.18 AS.seek 0.31ml @23:55:58.82 delay 3 seconds @23:56:01.84 ATV.dial :air3 @23:56:02.40 delay 3 seconds @23:56:05.48 AS.seek 0.335ml @23:56:10.80 delay 3 seconds @23:56:13.82 ATV.dial :bleach @23:56:14.46 AS.seek 0.32ml @23:56:17.78 delay 3 seconds @23:56:20.80 ATV.dial :extSrc @23:56:21.44 AS.seek 0ml @23:57:26.15 AFV.select :carrierSolution @23:57:26.27 delay 2 seconds @23:57:28.30 AS.reconfigure ASconfig @23:57:28.36 AS.seek 0.5ml @23:57:57.40 delay 1 second @23:57:58.42 AFV.select :holdingCoil @23:57:58.53 ATV.dial :extSrc @23:57:58.55 AS.reconfigure ASslow1 @23:57:58.63 AS.seek 0ml @23:59:39.56 AFV.select :carrierSolution @23:59:39.68 delay 2 seconds @23:59:41.71 AS.reconfigure ASconfig @23:59:41.77 AS.seek 0.5ml @00:00:10.81CEST25-Apr-20 delay 1 second @00:00:11.83 AFV.select :holdingCoil @00:00:11.95 ATV.dial :extSrc @00:00:12.31 AS.reconfigure ASslow1 @00:00:12.46 AS.seek 0ml @00:01:53.31 AFV.select :carrierSolution @00:01:53.43 delay 2 seconds @00:01:55.46 AS.reconfigure ASconfig @00:01:55.52 AS.seek 0.5ml @00:02:24.56 delay 1 second @00:02:25.58 AFV.select :holdingCoil @00:02:25.70 ATV.dial :extSrc @00:02:25.72 AS.reconfigure ASslow1 @00:02:25.80 AS.seek 0ml @00:04:06.64 AFV.select :carrierSolution @00:04:06.76 delay 2 seconds @00:04:08.80 AS.reconfigure ASconfig @00:04:08.91 AS.seek 0.5ml @00:04:38.00 delay 1 second @00:04:39.02 AFV.select :holdingCoil @00:04:39.14 ATV.dial :extSrc @00:04:39.16 AS.reconfigure ASslow1 @00:04:39.24 AS.seek 0ml @00:06:20.12 ATV.dial :air2 @00:06:21.47 AS.reconfigure ASslow0 @00:06:21.54 AS.seek 0.5ml @00:07:12.11 ATV.dial :extSrc @00:07:13.63 AS.seek 0.02ml @00:08:02.28 delay 1 second @00:08:03.30 ATV.dial :waste @00:08:04.62 AS.reconfigure ASconfig @00:08:04.68 AS.seek :empty @00:08:06.18 deconEluateRecoveryLine extSrc @00:08:06.19 ATV.dial :waste @00:08:06.23 AS.reconfigure ASnorm @00:08:06.30 AS.seek 0ml @00:08:06.71 ATV.dial :air3 @00:08:07.58 AS.reconfigure ASslow1 @00:08:07.65 AS.seek 0.01ml @00:08:09.95 delay 2 seconds @00:08:11.97 ATV.dial :bleach @00:08:12.46 AS.seek 0.31ml @00:09:13.09 delay 3 seconds @00:09:16.11 ATV.dial :air3 @00:09:16.70 delay 3 seconds @00:09:19.78 AS.seek 0.335ml @00:09:25.11 delay 3 seconds @00:09:28.13 ATV.dial :bleach @00:09:28.78 AS.seek 0.32ml @00:09:32.09 delay 3 seconds @00:09:35.11 ATV.dial :extSrc @00:09:35.76 AS.seek 0ml @00:10:40.40 AFV.select :carrierSolution @00:10:40.52 delay 2 seconds @00:10:42.55 AS.reconfigure ASconfig @00:10:42.61 AS.seek 0.5ml @00:11:11.67 delay 1 second @00:11:12.69 AFV.select :holdingCoil @00:11:12.81 ATV.dial :extSrc @00:11:12.83 AS.reconfigure ASslow1 @00:11:12.91 AS.seek 0ml @00:12:53.75 AFV.select :carrierSolution @00:12:53.87 delay 2 seconds @00:12:55.91 AS.reconfigure ASconfig @00:12:55.97 AS.seek 0.5ml @00:13:25.03 delay 1 second @00:13:26.05 AFV.select :holdingCoil @00:13:26.16 ATV.dial :extSrc @00:13:26.18 AS.reconfigure ASslow1 @00:13:26.26 AS.seek 0ml @00:15:07.09 AFV.select :carrierSolution @00:15:07.21 delay 2 seconds @00:15:09.24 AS.reconfigure ASconfig @00:15:09.30 AS.seek 0.5ml @00:15:38.32 delay 1 second @00:15:39.34 AFV.select :holdingCoil @00:15:39.45 ATV.dial :extSrc @00:15:39.47 AS.reconfigure ASslow1 @00:15:39.55 AS.seek 0ml @00:17:20.39 AFV.select :carrierSolution @00:17:20.51 delay 2 seconds @00:17:22.54 AS.reconfigure ASconfig @00:17:22.60 AS.seek 0.5ml @00:17:51.65 delay 1 second @00:17:52.67 AFV.select :holdingCoil @00:17:52.78 ATV.dial :extSrc @00:17:52.80 AS.reconfigure ASslow1 @00:17:52.88 AS.seek 0ml @00:19:33.71 ATV.dial :air2 @00:19:35.07 AS.reconfigure ASslow0 @00:19:35.13 AS.seek 0.5ml @00:20:25.71 ATV.dial :extSrc @00:20:27.22 AS.seek 0.02ml @00:21:15.84 delay 1 second @00:21:16.86 ATV.dial :waste @00:21:18.17 AS.reconfigure ASconfig @00:21:18.24 AS.seek :empty @00:21:19.80 Session Ended @12:22:41.30CEST01-May-20
ESP2 v3.30 I2C Gateway v3.06 for core bus @12:22:44.06 Copyright 2018 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @12:22:44.17 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @12:22:50.20 Polling Can every 10:00 @12:22:52.03 SPE.coast @12:22:52.16 SPE.configure SPEconfig @12:22:52.36 AS.reconfigure ASconfig @12:22:52.44 AS.hold @12:22:52.56 AS.configure ASconfig @12:22:52.76 SS.reconfigure SSconfig @12:22:52.84 SS.hold @12:22:52.96 SS.configure SSconfig @12:22:53.07 CC.reconfigure CCconfig @12:22:53.12 CC.hold @12:22:53.20 CC.configure CCconfig @12:22:53.30 CS.reconfigure CSconfig @12:22:53.35 CS.hold @12:22:53.42 CS.configure CSconfig @12:22:53.92 PC.reconfigure PCconfig @12:22:53.97 PC.hold @12:22:54.03 PC.configure PCconfig @12:22:54.14 PS.configure PSconfig @12:22:54.25 Forearm.reconfigure LooseForearm @12:22:54.30 Forearm.hold @12:22:54.35 Forearm.configure LooseForearm @12:22:54.44 Elbow.reconfigure LooseElbow @12:22:54.49 Elbow.hold @12:22:54.54 Elbow.configure LooseElbow @12:22:54.65 SC.reconfigure SCloose @12:22:54.70 SC.hold @12:22:54.76 SC.configure SCloose @12:22:54.86 SE.reconfigure SEconfig @12:22:54.91 SE.hold @12:22:55.02 SE.configure SEconfig @12:22:55.10 CH.coast @12:22:55.22 CH.configure CHconfig @12:22:55.29 PH.coast @12:22:55.41 PH.configure PHconfig @12:22:55.49 PCR.off @12:22:55.55 PCR.pidtemp= 50,0.1,0,300,25,25 @12:22:55.60 PCR.caltemp= 2.8814,0.0372,0 @12:22:55.64 All dwarf controllers running firmware version 3.75 @12:22:55.85 Loading /home/esp/esp2/mission/20invasivedeploy.rb mission @12:22:56.05 Session Begun @12:22:56.12
Preparing to sleep until +0:00:10 @12:22:56.16 GoodNews.email "Starting 20invasivedeploy w/pucks in tube 2\n{}\nCan@12:22:50, 20.8C, 38% humidity, 14.3psia, 11.928V, 0.771A, 0.771A avg, 9.19W",Subject: "20invasivedeploy begun" @12:22:56.39 AS.coast @12:22:56.43
Asleep til +0:00:10 @12:22:56.49 Continuing at 12:23 @12:22:56.55 SS.coast @12:22:56.66 CC.coast @12:22:56.71 CS.coast @12:22:56.77 PC.coast @12:22:56.84 Forearm.coast @12:22:56.90 Elbow.coast @12:22:56.97 SC.coast @12:22:57.03 SE.coast @12:22:57.46 Gate.power :core,:OFF @12:22:57.48 Gate.power :raw,:OFF @12:22:57.51 Shutdown @12:23:05.77 GoodNews.email "Then, in tube 2: coreNegNTCphase\n/home/esp/esp2/mission/20invasivedeploy.rb:119\n{}\nCan@12:22:56, 20.8C, 38% humidity, 14.3psia, 11.928V, 0.777A, 0.777A avg, 9.27W",Subject: "Asleep until 12:23" @12:23:06.00
Powering core bus @12:23:11.03 Gate.power :raw,:ON @12:23:12.87 SPE.coast @12:23:13.38 SPE.configure SPEconfig @12:23:13.62 AS.configure ASconfig @12:23:13.91 SS.configure SSconfig @12:23:14.16 CC.configure CCconfig @12:23:14.44 CS.configure CSconfig @12:23:14.78 PC.configure PCconfig @12:23:15.02 PS.configure PSconfig @12:23:15.39 Forearm.configure LooseForearm @12:23:15.86 Elbow.configure LooseElbow @12:23:16.38 SC.configure SCloose @12:23:16.82 SE.configure SEconfig @12:23:17.05 CH.coast @12:23:17.29 CH.configure CHconfig @12:23:17.44 PH.coast @12:23:17.73 PH.configure PHconfig @12:23:17.91 PCR.off @12:23:18.05 PCR.pidtemp= 50,0.1,0,300,25,25 @12:23:18.15 PCR.caltemp= 2.8814,0.0372,0 @12:23:18.34 All dwarf controllers running firmware version 3.75 @12:23:18.63 Valve.reset @12:23:19.16 PCR.off @12:23:19.29 PCR.pidtemp= 50,0.1,0,300,25,25 @12:23:19.44 PCR.caltemp= 2.8814,0.0372,0 @12:23:19.87 Intake.select :close @12:23:20.17 Exhaust.select :close @12:23:20.23 CTV.dial :air @12:23:20.65 PSV.dial :air @12:23:20.92 CSV.dial :waste1 @12:23:21.50 SSV.dial :spit,avoiding: :exhaust @12:23:22.70 PS.reconfigure PSconfig @12:23:23.03
Forearm.reconfigure LooseForearm @12:23:23.48 PS.home.seek 0ml @12:23:23.57
Forearm.home.seek :retract @12:23:24.21 CS.reconfigure CSfast @12:23:24.33 SS.reconfigure SSconfig @12:23:24.62 CS.home.seek 0ml @12:23:24.95 SS.home.seek 0ml @12:23:25.18 Hand.close @12:23:25.32
Elbow.jog 1500 @12:23:26.04 Elbow.reconfigure LooseElbow @12:23:26.25 Elbow.home.seek :clear @12:23:31.43 SE.reconfigure SEconfig @12:23:31.64 SE.home.seek :down @12:23:32.50 SC.reconfigure SCsloppy @12:23:32.71 SC.home.seek :initial @12:23:33.10 PS.coast @12:23:43.13
Forearm.seek :retract @12:23:43.46 Elbow.seek :hammer @12:23:44.90 Forearm.reconfigure TightForearm @12:23:45.11 Forearm.seek 5 + 350 counts @12:23:46.55 Forearm.seek :retract @12:23:48.42 PC.reconfigure PCconfig @12:23:48.51
CC.reconfigure CCconfig @12:23:48.81 PC.home.seek :open @12:23:48.93
CC.home.seek :open @12:23:53.43 Phase [coreNegNTCphase] @12:23:53.49 GoodNews.email "At 12:23:53 began in tube 2: coreNegNTCphase\n/home/esp/esp2/mission/20invasivedeploy.rb:119\n{}\nCan@12:23:53, 20.8C, 39% humidity, 14.3psia, 11.937V, 0.733A, 0.727A avg, 8.75W",Subject: "Started 20invasivedeploy phase coreNegNTCphase" @12:23:53.62CEST01-May-20
===> STARTED coreNegNTC (phase #1) <=== Everybody has a plan untill they get punched in the face - Mike Tyson @12:23:54.10 Starting mfb @12:23:54.15 pcrBAC wcr sampling at most 50ml @12:23:54.50 Loading sh1 puck from tube 2 into Collection Clamp @12:23:54.86 Readying the MFB @12:23:54.92
Hand.open @12:23:54.99 Homing MFB Syringe @12:23:55.20 AFV.select :holdingCoil @12:23:55.70 ATV.dial :air2 @12:23:57.36
SC.reconfigure SCloose @12:23:57.48 AS.reconfigure ASconfig @12:23:58.24 AS.home.seek :home @12:23:58.90
Elbow.reconfigure TightElbow @12:23:59.15 Elbow.seek 2 @12:24:00.39 Forearm.seek 2 + 700 counts @12:24:01.80 SE.reconfigure SEpick @12:24:02.19 SE.seek beamBreak @12:24:04.02 AFV.select :carrierSolution @12:24:04.25 delay 2 seconds @12:24:05.51
SE.configure SEconfig @12:24:06.45 Elbow.seek 2 @12:24:06.78 AS.seek 1ml @12:24:07.49
Forearm.seek 2 + 300 counts @12:24:07.91 Forearm.reconfigure LooseForearm @12:24:08.13 Hand.close @12:24:09.11 SE.seek :down @12:24:09.97
Forearm.seek :retract @12:24:11.14 Elbow.seek CC @12:24:12.09 SE.coast @12:24:12.97
Elbow.reconfigure LooseElbow @12:24:13.10 Forearm.reconfigure TightForearm @12:24:13.17 Forearm.seek CC @12:24:14.60 CC.seek :closed @12:24:19.31 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.49 @12:24:19.36 Hand.open @12:24:19.81 I2C::UnexpectedReply in core -- I2C::Servo::Status3Reply[6F:27->20] @12:24:20.59 Forearm.reconfigure LooseForearm @12:24:20.92 Forearm.seek :retract @12:24:22.14 SC.coast @12:24:22.27 skipping prime and sample collection @12:24:22.31 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @12:24:22.36 Intake.select :close @12:24:22.58 CSV.dial :waste @12:24:22.68 CS.seek 0ml @12:24:23.06 CSV.dial :air @12:24:23.17
Calibrating pressure and temperature sensors @12:24:23.27 CTV.dial :air @12:24:23.42 SSV.dial :spit,avoiding: :exhaust @12:24:23.50 SS.reconfigure SSfast @12:24:24.08 SS.seek 5ml @12:24:24.20 CS.seek 10ml @12:24:26.18 BACfans.email "Started mfb in tube 2 at 12:23:54CEST01-May-20\n{}\nCan@12:23:53, 20.8C, 39% humidity, 14.3psia, 11.937V, 0.733A, 0.727A avg, 8.75W",Subject: "pcrBAC wcr sampling at most 50ml" @12:24:27.24 SSV.dial CBV @12:24:27.41
CBV.dial SSV @12:24:29.36 delay 3.8 seconds @12:24:33.91 SSV.dial :spit @12:24:35.85 SS.seek 0ml @12:24:38.69 SSV.dial CBV @12:24:40.67 SSV.dial CBV,avoiding: :exhaust @12:24:40.80
CBV.dial SSV @12:24:40.95 CTV.dial :air @12:24:41.02 delay 15 seconds @12:24:56.16 SSV.dial :spit,avoiding: :exhaust @12:24:57.99 CTV.dial CSV,avoiding: :intake @12:24:58.21
SS.reconfigure SSexhale @12:24:58.65 SS.seek 0ml @12:24:59.22 SSV.dial CBV,avoiding: :exhaust @12:24:59.86 delay 1 second @12:25:01.18 AFV.select :holdingCoil @12:25:01.51
CBV.dial between SSV and waste2 @12:25:01.79 delay 1 second @12:25:02.40 CTV.dial CSV,avoiding: :air @12:25:02.52
CSV.dial CTV @12:25:02.85 ATV.dial :waste @12:25:03.31
CS.reconfigure CSpressurize @12:25:03.62 CS.seek 0ml @12:25:04.30 AS.reconfigure ASnorm @12:25:04.59 AS.home.seek :empty @12:25:30.37
CSV.dial :air @12:25:31.10 CS.reconfigure CSfast @12:25:31.33 CS.seek 10ml @12:25:57.30 ATV.dial :waste @12:25:57.31 ARV.dial between carrier and air1 @12:25:57.44 ASV.dial :waste1 @12:25:58.39
CSV.dial between air and CTV @12:25:58.79 CS.seek 3.368ml @12:26:16.89 CTV.dial CSV,avoiding: :air @12:26:16.93
CSV.dial CTV @12:26:17.32 CS.reconfigure CSpressurize @12:26:17.39 CS.seek 0ml @12:26:26.86 CSV.dial :air @12:26:27.42 CS.reconfigure CSfast @12:26:27.50 CS.seek 10ml @12:26:54.23 CSV.dial between air and CTV @12:26:54.64 CS.seek 2.101ml @12:27:16.02 CTV.dial CSV,avoiding: :air @12:27:16.05
CSV.dial CTV @12:27:16.45 CS.reconfigure CSpressurize @12:27:16.52 CS.seek 0ml @12:27:18.01 BubblePoint: Intake @69.5psia, Outlet @14.6psia @12:27:18.03 CBV.dial SSV @12:27:18.44 SS.reconfigure SSconfig @12:27:18.98 SS.seek 10ml @12:27:25.08 delay 15 seconds @12:27:40.23 Evacuated: Intake @28.5psia, Outlet @28.7psia @12:27:40.24 SSV.dial :spit,avoiding: :exhaust @12:27:41.83 SS.reconfigure SSexhale @12:27:41.91 SS.seek 0ml @12:27:46.94 SS.reconfigure SSfast @12:27:47.02 SS.seek 5ml @12:27:50.02 SSV.dial CBV,avoiding: :exhaust @12:27:50.06
CBV.dial SSV @12:27:51.63 CTV.dial :air,avoiding: :intake @12:27:53.27 CSV.dial CTV @12:27:53.32
SS.seek 10ml @12:27:54.32 CSV.dial :air @12:27:54.88 CS.reconfigure CSfast @12:27:54.96 CS.seek 0ml @12:27:56.33
SSV.dial :spit,avoiding: :exhaust @12:27:57.91 SS.reconfigure SSexhale @12:27:57.98 SS.seek 0ml @12:28:00.57 CS.coast @12:28:03.03
SS.reconfigure SSfast @12:28:03.12 SS.seek 5ml @12:28:05.91 SSV.dial CBV,avoiding: :exhaust @12:28:05.95
CBV.dial SSV @12:28:08.51 SSV.dial :spit,avoiding: :exhaust @12:28:10.08 SS.reconfigure SSconfig @12:28:10.15 SS.seek 0ml @12:28:13.37 SS.coast @12:28:13.44 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @12:28:13.45 Intake.select :close @12:28:13.58 CSV.dial :waste @12:28:13.72 SSV.dial CBV,avoiding: :exhaust @12:28:13.76
CBV.dial SSV @12:28:14.51 CS.seek 0ml @12:28:14.61 CSV.dial :air @12:28:15.32
CTV.dial :air @12:28:15.34 delay 15 seconds @12:28:15.62 CS.seek 10ml @12:28:30.38
SSV.dial :spit,avoiding: :exhaust @12:28:31.94 CTV.dial CSV,avoiding: :intake @12:28:31.98
SS.reconfigure SSexhale @12:28:32.11 SS.seek 0ml @12:28:32.20 SSV.dial CBV,avoiding: :exhaust @12:28:42.80 CBV.dial between SSV and waste2 @12:28:43.28 CTV.dial CSV,avoiding: :air @12:28:43.31
CSV.dial CTV @12:28:43.91 CS.reconfigure CSpressurize @12:28:43.98 CS.seek 0ml @12:29:10.21 CSV.dial :air @12:29:10.77 CS.reconfigure CSfast @12:29:10.85 CS.seek 10ml @12:29:37.55 CSV.dial between air and CTV @12:29:37.97 CS.seek 3.342ml @12:29:56.11 CTV.dial CSV,avoiding: :air @12:29:56.14
CSV.dial CTV @12:29:56.55 CS.reconfigure CSpressurize @12:29:56.62 CS.seek 0ml @12:30:06.05 CSV.dial :air @12:30:06.62 CS.reconfigure CSfast @12:30:06.69 CS.seek 10ml @12:30:33.36 CSV.dial between air and CTV @12:30:33.76 CS.seek 2.09ml @12:30:55.06 CTV.dial CSV,avoiding: :air @12:30:55.08
CSV.dial CTV @12:30:55.47 CS.reconfigure CSpressurize @12:30:55.55 CS.seek 0ml @12:30:57.12 BubblePoint: Intake @70.2psia, Outlet @15.1psia @12:30:57.13 CBV.dial SSV @12:30:57.56 SS.reconfigure SSconfig @12:30:57.63 SS.seek 10ml @12:31:04.27 delay 15 seconds @12:31:19.41 Evacuated: Intake @28.6psia, Outlet @29.0psia @12:31:19.42 SSV.dial :spit,avoiding: :exhaust @12:31:21.00 SS.reconfigure SSexhale @12:31:21.08 SS.seek 0ml @12:31:26.25 SS.reconfigure SSfast @12:31:26.33 SS.seek 5ml @12:31:29.00 SSV.dial CBV,avoiding: :exhaust @12:31:29.04
CBV.dial SSV @12:31:30.61 CTV.dial :air,avoiding: :intake @12:31:32.24 CSV.dial CTV @12:31:32.30
SS.seek 10ml @12:31:33.29 CSV.dial :air @12:31:33.85 CS.reconfigure CSfast @12:31:33.93 CS.seek 0ml @12:31:35.29
SSV.dial :spit,avoiding: :exhaust @12:31:36.86 SS.reconfigure SSexhale @12:31:36.93 SS.seek 0ml @12:31:39.61 CS.coast @12:31:42.40
SS.reconfigure SSfast @12:31:42.48 SS.seek 5ml @12:31:45.18 SSV.dial CBV,avoiding: :exhaust @12:31:45.21
CBV.dial SSV @12:31:47.81 SSV.dial :spit,avoiding: :exhaust @12:31:49.37 SS.reconfigure SSconfig @12:31:49.44 SS.seek 0ml @12:31:52.87 SS.coast @12:31:52.99 Lysing: Adding 1.5ml lysis @12:31:53.01 CBV.dial between CSV and waste1,avoiding: :DBV @12:31:54.80 CTV.dial CSV @12:31:56.40 CSV.dial CSR @12:31:57.35 Pulling 1ml of CSR.lysis @12:31:57.37 CSR.select :lysis @12:31:57.53 CS.reconfigure CSconfig @12:31:57.60 CS.seek 1ml @12:32:01.18 delay 5 seconds @12:32:06.21 CSR.select :air @12:32:06.42 CS.seek 6ml @12:32:20.24 delay 2 seconds @12:32:22.26 CSV.dial :waste1 @12:32:23.84 CS.reconfigure CSfast @12:32:23.91 CS.seek :empty @12:32:40.37 CSV.dial CSR @12:32:42.00 Pulling 1.5ml of CSR.lysis @12:32:42.01 CSR.select :lysis @12:32:42.17 CS.reconfigure CSconfig @12:32:42.24 CS.seek 1.5ml @12:32:47.21 delay 5 seconds @12:32:52.24 CSR.select :air @12:32:52.44 CS.seek 4.501ml @12:33:01.15 delay 2 seconds @12:33:03.17 CSV.dial CTV @12:33:03.75 CS.seek 1.275ml @12:33:13.03 Heating sh1 puck from tube 2 in Collection Clamp to 85C for 5:00 @12:33:13.05 CH.seek 85C,8:00 @12:36:52.82 CH.hold 5:00,85C @12:41:53.01 Collection Puck Heater at 85C after lysing @12:41:53.03 CS.seek 0ml @12:41:57.37 CTV.dial between spare and air,avoiding: :intake @12:41:58.64 CSV.dial :air @12:41:59.19 CS.seek 5ml @12:42:13.12 CSV.dial CTV @12:42:13.68 delay 2 seconds @12:42:15.70 CSV.dial :air @12:42:16.26 delay 2 seconds @12:42:18.28 CSV.dial CTV @12:42:18.84 delay 2 seconds @12:42:20.87 CSV.dial :air @12:42:21.54 delay 2 seconds @12:42:23.57 CS.seek 0ml @12:42:37.47 Recovering Lysate into Collection syringe @12:42:37.48 CSV.dial CBV @12:42:38.75 CBV.dial CSV @12:42:39.21 CS.seek 5ml @12:42:53.09 delay 5 seconds @12:42:58.12 CTV.dial :air @12:42:58.74 CS.seek 7ml @12:43:05.00 delay 5 seconds @12:43:10.03 CSV.dial :air2 @12:43:10.57 CS.seek 10ml @12:43:19.28 CSV.dial between air and CTV,avoiding: CBV @12:43:20.67 Lysing Complete @12:43:20.78 Storing sh1 puck in Collection Clamp to tube 1 @12:43:20.98 SC.reconfigure SCtight @12:43:21.05 SC.seek 1 @12:43:23.22 SC.reconfigure SCloose @12:43:23.47 Elbow.reconfigure TightElbow @12:43:23.54 Elbow.seek 1 @12:43:25.42 Forearm.reconfigure TightForearm @12:43:25.50 Forearm.seek 1 + 700 counts @12:43:26.60 SE.reconfigure SEpick @12:43:26.71 SE.seek beamBreak @12:43:29.73 SE.configure SEconfig @12:43:30.32 SE.seek :place @12:43:30.95 Forearm.reconfigure LooseForearm @12:43:31.02 Forearm.seek :retract @12:43:32.00 Elbow.seek CC @12:43:33.74 Elbow.reconfigure LooseElbow @12:43:33.88 Forearm.seek CC @12:43:34.96 Hand.close @12:43:35.79 CC.seek :open @12:43:40.34 Forearm.seek :retract @12:43:41.45 Elbow.reconfigure TightElbow @12:43:41.53 Elbow.seek 1 @12:43:43.53 Elbow.reconfigure LimpElbow @12:43:43.61 Forearm.seek 1 @12:43:44.68 Elbow.hold @12:43:44.78 Elbow.reconfigure TightElbow @12:43:44.85 Hand.open @12:43:45.78 Forearm.force -20 @12:43:45.85 Forearm.seek 1 + 343 counts @12:43:46.20 Forearm.reconfigure TightForearm @12:43:46.28 Forearm.seek 1 + 700 counts @12:43:46.78 SE.seek :down @12:43:47.92
Forearm.reconfigure LooseForearm @12:43:48.00 Forearm.seek :retract @12:43:49.68 SE.coast @12:43:49.74
SC.coast @12:43:49.80 Tranferring lysate to PRV @12:43:49.82 CSV.dial :air @12:43:50.19 CS.seek 10ml @12:43:50.28 CSV.dial between air and CTV @12:43:50.65 AIV.dial :bypass @12:43:50.76 Beginning samplePassC @12:43:50.78 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:43:50.89 Hand.open @12:43:51.28 Elbow.seek FlushPuck::Garage @12:43:53.38 Elbow.reconfigure LooseElbow @12:43:53.52 Forearm.seek :garage @12:43:54.61 Hand.close @12:43:55.53 Forearm.seek :retract @12:43:56.61 Elbow.reconfigure TightElbow @12:43:56.69 Elbow.seek CC @12:43:59.62 Elbow.reconfigure LooseElbow @12:43:59.75 Forearm.reconfigure TightForearm @12:43:59.82 Forearm.seek CC @12:44:01.17 CC.seek :closed @12:44:05.53 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.46 @12:44:06.07 Hand.open @12:44:06.97 Forearm.reconfigure LooseForearm @12:44:07.04 Forearm.seek :retract @12:44:08.11 AWV.select :air @12:44:08.12 AIV.dial :bypass @12:44:08.16 PSV.dial PSR @12:44:09.77 Pulling 1.5ml of PSR.flush @12:44:09.78 PSR.select :flush @12:44:09.95 PS.seek 1.5ml @12:44:26.48 delay 5 seconds @12:44:31.51 PSV.dial :waste1 @12:44:32.07 PS.seek 0ml @12:44:48.70 PSV.dial PSR @12:44:49.25 PSR.select :air @12:44:49.40 PS.seek 2.5ml @12:45:16.04 PRV.dial CBV @12:45:16.08
CBV.dial PRV @12:45:17.06 PSV.dial :mixloop @12:45:18.25 CTV.dial :air,avoiding: :intake @12:45:18.27 PTV.dial :PRVmixing @12:45:19.16 PS.seek 0ml @12:45:45.98 PSV.dial :air @12:45:46.51 PS.seek 2.5ml @12:46:13.17 PSV.dial :mixloop @12:46:13.71 PS.seek 0ml @12:46:40.64 PSV.dial :air @12:46:41.18 PS.seek 2.5ml @12:47:07.79 PSV.dial :mixloop @12:47:08.34 PS.seek 0ml @12:47:35.17 Garaging FlushPuck in Collection Clamp @12:47:35.28 Elbow.reconfigure TightElbow @12:47:35.35 Elbow.seek CC @12:47:35.64 Elbow.reconfigure LooseElbow @12:47:35.78 Forearm.seek CC @12:47:36.88 Hand.close @12:47:37.66 CC.seek :open @12:47:42.10 Forearm.seek :retract @12:47:43.20 Elbow.reconfigure TightElbow @12:47:43.28 Elbow.seek :garage @12:47:46.48 Elbow.reconfigure LooseElbow @12:47:46.61 Forearm.reconfigure TightForearm @12:47:46.68 Forearm.seek :garage @12:47:47.85 Hand.open @12:47:48.73 Forearm.reconfigure LooseForearm @12:47:48.80 Forearm.seek :retract @12:47:49.91 Connecting PS to CS to transfer sample @12:47:49.92 CBV.dial AIV @12:47:49.96 AIV.dial :bypass @12:47:49.99 PSV.dial :mixloop @12:47:50.01 PTV.dial :PRVmixing @12:47:50.04 PRV.dial AIV @12:47:50.06 CTV.dial :air,avoiding: :intake @12:47:50.10 Loading lyfil puck from tube 2 into Collection Clamp @12:47:50.38 SC.reconfigure SCtight @12:47:50.46 SC.seek 2 @12:47:52.64 SC.reconfigure SCloose @12:47:52.89 Elbow.reconfigure TightElbow @12:47:52.97 Elbow.seek 2 @12:47:55.13 Forearm.reconfigure TightForearm @12:47:55.21 Forearm.seek 2 + 700 counts @12:47:56.32 SE.reconfigure SEpick @12:47:56.42 SE.seek beamBreak @12:47:59.55 SE.configure SEconfig @12:47:59.74 Elbow.seek 2 @12:47:59.97 Forearm.seek 2 + 300 counts @12:48:00.24 Forearm.reconfigure LooseForearm @12:48:00.31 Hand.close @12:48:01.05 SE.seek :down @12:48:01.29
Forearm.seek :retract @12:48:02.80 Elbow.seek CC @12:48:03.90 SE.coast @12:48:04.53
Elbow.reconfigure LooseElbow @12:48:04.66 Forearm.reconfigure TightForearm @12:48:04.73 Forearm.seek CC @12:48:06.08 CC.seek :closed @12:48:10.39 Collection Clamp onSpring/offSpring for 5/31 samples, on/off torque 2.45 @12:48:10.40 Hand.open @12:48:11.23 Forearm.reconfigure LooseForearm @12:48:11.30 Forearm.seek :retract @12:48:12.39 SC.coast @12:48:12.45 CTV.dial CSV,avoiding: :intake @12:48:14.06 CSV.dial CTV @12:48:14.45 CS.seek 5ml @12:48:28.34 delay 1:20 @12:49:48.38 CS.seek 2.5ml @12:49:56.09 delay 40 seconds @12:50:36.12 CS.seek 0ml @12:50:43.72 delay 1:00 @12:51:43.75 PSV.dial :air @12:51:44.26 PS.seek 2.5ml @12:52:10.95 PSV.dial :mixloop @12:52:11.47 delay 3 seconds @12:52:14.50 PSV.dial :air @12:52:15.00 delay 2 seconds @12:52:17.02 PSV.dial :mixloop @12:52:17.66 delay 3 seconds @12:52:20.69 PSV.dial :waste1 @12:52:22.19 PS.seek :empty @12:52:49.07 CBV.dial :waste1 @12:52:49.64 delay 5 seconds @12:52:54.66 CBV.dial AIV @12:52:55.21 delay 10 seconds @12:53:05.24 CBV.dial :waste1 @12:53:05.80 delay 10 seconds @12:53:15.83 CBV.dial AIV @12:53:16.38 delay 10 seconds @12:53:26.41 CTV.dial CSV,avoiding: :intake @12:53:26.42 CBV.dial between AIV and waste1 @12:53:26.83 CSV.dial :air @12:53:27.40 CS.seek 10ml @12:53:54.19 CSV.dial CTV @12:53:54.75 CS.seek 0ml @12:54:21.74 CBV.dial :waste1 @12:54:22.13 delay 10 seconds @12:54:32.16 CTV.dial :air,avoiding: :intake @12:54:33.83 Storing lyfil puck in Collection Clamp to tube 1 @12:54:34.04 SC.reconfigure SCtight @12:54:34.11 SC.seek 1 @12:54:36.29 SC.reconfigure SCloose @12:54:36.54 Elbow.reconfigure TightElbow @12:54:36.61 Elbow.seek 1 @12:54:38.54 Forearm.reconfigure TightForearm @12:54:38.62 Forearm.seek 1 + 700 counts @12:54:39.71 SE.reconfigure SEpick @12:54:39.82 SE.seek beamBreak @12:54:43.05 SE.configure SEconfig @12:54:43.25 Elbow.reconfigure LimpElbow @12:54:43.32 Forearm.reconfigure LooseForearm @12:54:43.39 Forearm.seek 1 @12:54:44.16 Elbow.hold @12:54:44.35 Elbow.reconfigure TightElbow @12:54:44.48 SE.seek 0.07 Pucks below Top Plate @12:54:44.95 Forearm.force -20 @12:54:45.02 Forearm.seek 1 + 346 counts @12:54:45.37 Forearm.reconfigure TightForearm @12:54:45.45 Forearm.seek 1 + 700 counts @12:54:45.85 SE.seek 1.07 Pucks below Top Plate @12:54:46.60 Forearm.reconfigure LooseForearm @12:54:46.67 Forearm.seek :retract @12:54:47.62 Elbow.seek CC @12:54:49.30 Elbow.reconfigure LooseElbow @12:54:49.43 Forearm.seek CC @12:54:50.51 Hand.close @12:54:51.29 CC.seek :open @12:54:55.85 Forearm.seek :retract @12:54:56.92 Elbow.reconfigure TightElbow @12:54:57.00 Elbow.seek 1 @12:54:59.01 Elbow.reconfigure LimpElbow @12:54:59.09 Forearm.seek 1 @12:55:00.17 Elbow.hold @12:55:00.28 Elbow.reconfigure TightElbow @12:55:00.34 Hand.open @12:55:01.30 Forearm.force -20 @12:55:01.37 Forearm.seek 1 + 336 counts @12:55:01.72 Forearm.reconfigure TightForearm @12:55:01.80 Forearm.seek 1 + 700 counts @12:55:02.34 SE.seek :down @12:55:03.50
Forearm.reconfigure LooseForearm @12:55:03.58 Forearm.seek :retract @12:55:05.17 SE.coast @12:55:05.23
SC.coast @12:55:05.29 PSV.dial :air @12:55:06.55 PS.seek 0.499ml @12:55:12.58 delay 3 seconds @12:55:15.60 PSV.dial :mixloop @12:55:16.11 PRV.dial :air @12:55:16.64 delay 3 seconds @12:55:19.66 PRV.dial CBV @12:55:20.20 CBV.dial AIV @12:55:20.75 PRV.dial between CBV and air @12:55:21.13 PRV.dial :air @12:55:21.56 PS.reconfigure PSslow @12:55:21.63 PS.seek 0.599ml @12:55:24.27 PRV.dial :waste2 @12:55:25.14 PS.reconfigure PSconfig @12:55:25.21 PS.seek :empty @12:55:32.17 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:55:32.27 Hand.open @12:55:32.67 Elbow.seek FlushPuck::Garage @12:55:34.73 Elbow.reconfigure LooseElbow @12:55:34.87 Forearm.seek :garage @12:55:36.43 Hand.close @12:55:37.31 Forearm.seek :retract @12:55:38.40 Elbow.reconfigure TightElbow @12:55:38.48 Elbow.seek CC @12:55:41.43 Elbow.reconfigure LooseElbow @12:55:41.56 Forearm.reconfigure TightForearm @12:55:41.63 Forearm.seek CC @12:55:43.04 CC.seek :closed @12:55:47.32 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.29 @12:55:47.42 Hand.open @12:55:48.24 Forearm.reconfigure LooseForearm @12:55:48.31 Forearm.seek :retract @12:55:49.45 Clearing PML for modified lysate @12:55:49.46 CTV.dial :air,avoiding: :intake @12:55:49.50 PRV.dial :waste2 @12:55:49.53 PTV.dial :PRV,avoiding: :puck @12:55:50.43 delay 2 seconds @12:55:52.45 PTV.dial :PRVmixing,avoiding: :puck @12:55:53.32 delay 2 seconds @12:55:55.35 PSV.dial :PML @12:55:55.37 delay 2 seconds @12:55:57.41 PRV.dial :air @12:55:58.28 delay 2 seconds @12:56:00.37 PS.seek 1.499ml @12:56:16.83 delay 5 seconds @12:56:21.87 PS.seek 2.5ml @12:56:33.05 PSV.dial :air @12:56:33.59 PS.seek 0ml @12:57:00.30 mixing lysate with speDil @12:57:00.31 PSV.dial :PML @12:57:00.86 CBV.dial :PRV @12:57:00.87 PRV.dial :air @12:57:00.91 delay 2 seconds @12:57:02.95 PRV.dial :speDil @12:57:03.50 delay 2 seconds @12:57:05.59 PS.reconfigure PSslow1 @12:57:05.67 PS.seek 0.225ml @12:57:29.64 delay 5 seconds @12:57:34.67 PRV.dial CBV @12:57:35.55 delay 2 seconds @12:57:37.65 PS.seek 0.475ml @12:58:03.33 delay 5 seconds @12:58:08.36 PRV.dial :air @12:58:08.91 delay 2 seconds @12:58:10.94 PS.seek 0.965ml @12:59:01.24 delay 10 seconds @12:59:11.27 PRV.dial CBV @12:59:11.81 CTV.dial :air,avoiding: :intake @12:59:11.83 PSV.dial :air @12:59:12.39 PS.reconfigure PSconfig @12:59:12.46 PS.seek 2.5ml @12:59:29.11 delay 3 seconds @12:59:32.13 PSV.dial PTV @12:59:33.00 PTV.dial :PRV,avoiding: :puck @12:59:33.87 PS.seek 0ml @13:00:00.52 PSV.dial :air @13:00:01.39 PS.seek 2.5ml @13:00:27.99 delay 3 seconds @13:00:31.01 PSV.dial PTV @13:00:31.86 PTV.dial :PRV,avoiding: :puck @13:00:31.89 PS.seek 0ml @13:00:58.92 Pushing modified lysate into AIV Injection Loop @13:00:58.93 CBV.dial AIV @13:00:58.97 AIV.dial :bypass @13:00:59.00 PRV.dial CBV @13:00:59.03 PSV.dial :air @13:00:59.92 PS.seek 2.5ml @13:01:26.55 delay 3 seconds @13:01:29.57 PSV.dial :PML @13:01:30.10 PTV.dial :PRVmixing,avoiding: :puck @13:01:31.04 PS.reconfigure PSslow1 @13:01:31.11 PS.seek 1.74ml @13:02:48.58 delay 5 seconds @13:02:53.60 AIV.dial :sample @13:02:54.29 AWV.select :waste @13:02:54.49 PS.reconfigure PSslow2 @13:02:54.56 PS.seek 1.22ml @13:04:41.86 delay 15 seconds @13:04:56.89 AIV.dial :bypass @13:04:57.57 PS.reconfigure PSconfig @13:04:57.65 PS.seek 0ml @13:05:11.11 PSV.dial :air @13:05:11.66 PS.seek 2.5ml @13:05:38.11 PSV.dial :PML @13:05:38.66 PS.seek 0ml @13:06:05.33 AIV.dial :coilToMFB @13:06:05.34 AWV.select :air @13:06:05.55 Extracting Nucleic Acid from AIV @13:06:05.58
Beginning samplePassD @13:06:05.63 SPE.hold 2:46:40,55C @13:06:05.73 ASV.dial :SPEdelivery @13:06:05.84
AWV.select :air @13:06:05.86 AIV.dial :bypass @13:06:05.89 PSV.dial PSR @13:06:06.21 delay 20 seconds @13:06:07.25
Pulling 1.5ml of PSR.killMFB @13:06:07.26 PSR.select :killMFB @13:06:07.43 PS.seek 1.5ml @13:06:24.71 delay 5 seconds @13:06:26.24 primeLysateINT @13:06:26.26 AWV.select :air @13:06:26.29 AIV.dial :coilToMFB @13:06:26.32 ATV.dial AIV @13:06:28.29 AS.reconfigure ASslow1 @13:06:28.36 AS.seek 0.299ml @13:06:29.74
PSV.dial :waste1 @13:06:30.31 PS.seek 0ml @13:06:47.71 PSV.dial PSR @13:06:48.27 PSR.select :air @13:06:48.42 PS.seek 2.5ml @13:07:16.71 PRV.dial CBV @13:07:16.74
CBV.dial PRV @13:07:16.78 PSV.dial :mixloop @13:07:18.04 CTV.dial :air,avoiding: :intake @13:07:18.05 PTV.dial :PRVmixing @13:07:18.10 PS.seek 0ml @13:07:29.03 delay 10 seconds @13:07:39.06 ATV.dial :air2 @13:07:39.99 AS.seek 0.329ml @13:07:46.32
PSV.dial PSR @13:07:46.43 ATV.dial :waste2 @13:07:47.06 AS.reconfigure ASnorm @13:07:47.13 AS.seek :empty @13:07:47.66
Pulling 1.5ml of PSR.flush @13:07:47.67 PSR.select :flush @13:07:47.85 PS.seek 1.5ml @13:08:04.13 Lysate primed! @13:08:04.14 AFV.select :holdingCoil @13:08:04.17 ATV.dial :air2 @13:08:04.88 AS.reconfigure ASslow1 @13:08:04.95 AS.seek 0.069ml @13:08:05.08
delay 5 seconds @13:08:10.12 PSV.dial :waste1 @13:08:10.69 PS.seek 0ml @13:08:19.35 delay 3 seconds @13:08:22.37 ATV.dial :AIV @13:08:23.31 AS.seek 0.469ml @13:08:28.04
PSV.dial PSR @13:08:28.61 PSR.select :air @13:08:29.28 PS.seek 2.5ml @13:08:57.52 PRV.dial CBV @13:08:57.55
CBV.dial PRV @13:08:57.59 PSV.dial :mixloop @13:08:58.83 CTV.dial :air,avoiding: :intake @13:08:58.85 PTV.dial :PRVmixing @13:08:58.91 PS.seek 0ml @13:09:26.93 PSV.dial PSR @13:09:28.24 Pulling 1.5ml of PSR.flush @13:09:28.25 PSR.select :flush @13:09:28.42 PS.seek 1.5ml @13:09:44.03 delay 5 seconds @13:09:45.40
delay 5 seconds @13:09:49.12 Solid Phase Extraction Heater at 47.8C @13:09:49.13 ATV.dial ASV @13:09:49.58 ASV.dial :SPEdelivery @13:09:49.68 AS.seek 0.041ml @13:09:50.43
PSV.dial :waste1 @13:09:50.97 PS.seek 0ml @13:10:07.87 PSV.dial PSR @13:10:08.41 PSR.select :air @13:10:08.57 PS.seek 2.5ml @13:10:36.12 PRV.dial CBV @13:10:36.15
CBV.dial PRV @13:10:36.19 PSV.dial :mixloop @13:10:37.43 CTV.dial :air,avoiding: :intake @13:10:37.45 PTV.dial :PRVmixing @13:10:37.51 PS.seek 0ml @13:11:05.02 PSV.dial PSR @13:11:06.30 Pulling 1.5ml of PSR.flush @13:11:06.32 PSR.select :flush @13:11:06.48 PS.seek 1.5ml @13:11:16.08 delay 10 seconds @13:11:23.12
delay 5 seconds @13:11:26.11 rinseLysateLineINT @13:11:26.12 AFV.select :carrierSolution @13:11:26.26 delay 2 seconds @13:11:28.15
PSV.dial :waste1 @13:11:28.28 AS.reconfigure ASconfig @13:11:28.35 AS.seek 1ml @13:11:28.70
PS.seek 0ml @13:11:45.33 PSV.dial PSR @13:11:45.88 PSR.select :air @13:11:46.03 PS.seek 2.5ml @13:12:13.18 PRV.dial CBV @13:12:13.22
CBV.dial PRV @13:12:13.25 PSV.dial :mixloop @13:12:14.46 CTV.dial :air,avoiding: :intake @13:12:14.47 PTV.dial :PRVmixing @13:12:14.52 PS.reconfigure PSslow @13:12:14.60 PS.seek 1.2ml @13:12:23.78 delay 1 second @13:12:24.80 AFV.select :holdingCoil @13:12:24.92 delay 1 second @13:12:25.95 ATV.dial AIV @13:12:26.53 AIV.dial :coilToMFB @13:12:26.55 AWV.select :waste @13:12:26.69 AS.reconfigure ASslow0 @13:12:26.77 AS.seek 0ml @13:12:41.46
PSV.dial :waste1 @13:12:43.02 PS.reconfigure PSconfig @13:12:43.09 PS.seek 0ml @13:12:56.45 PSV.dial :air @13:12:57.81 PS.seek 2.5ml @13:13:24.46 PSV.dial :mixloop @13:13:25.01 PS.reconfigure PSslow @13:13:25.10 PS.seek 0ml @13:14:07.70 ATV.dial :air2 @13:14:08.43 AS.reconfigure ASslow1 @13:14:08.50 AS.seek 0.05ml @13:14:16.27
PSV.dial :air @13:14:16.81 PS.reconfigure PSconfig @13:14:16.88 PS.seek 2.5ml @13:14:18.88 ATV.dial :AIV @13:14:19.76 AS.seek 0.01ml @13:14:28.13 ATV.dial :waste @13:14:29.85 AS.seek :empty @13:14:32.31 ethanol wash @13:14:32.32 ATV.dial :air2 @13:14:34.18 AS.reconfigure ASslow2 @13:14:34.26 AS.seek 0.009ml @13:14:43.84
PSV.dial :mixloop @13:14:44.37 PS.reconfigure PSslow @13:14:44.46 PS.seek 0ml @13:14:44.72 delay 3 seconds @13:14:47.74 Pulling 0.4ml of Ethanol Wash @13:14:47.75 ATV.dial :ethanol @13:14:48.65 AS.reconfigure ASslow1 @13:14:48.72 AS.seek 0.409ml @13:15:35.77
Cleaning Collection Syringe @13:15:35.78 CSV.dial CSR @13:15:36.40 Pulling 4ml of CSR.flush @13:15:36.41 CSR.select :flush @13:15:36.57 CS.seek 4ml @13:15:47.99 delay 5 seconds @13:15:53.02 CSR.select :air @13:15:53.18 CS.seek 10ml @13:16:09.47 delay 3 seconds @13:16:09.74 CSV.dial CTV @13:16:09.78
CTV.dial CSV @13:16:11.47 CBV.dial :waste1 @13:16:12.04 CS.seek 0ml @13:16:12.49 ATV.dial :air1 @13:16:12.92 delay 3 seconds @13:16:16.01 AS.seek 0.434ml @13:16:21.39 delay 3 seconds @13:16:24.42 ATV.dial :ethanol @13:16:25.07 AS.seek 0.419ml @13:16:28.39 delay 3 seconds @13:16:31.42 ATV.dial ASV @13:16:32.86 AS.seek 0.092ml @13:16:38.83
CSV.dial CSR @13:16:39.56 Pulling 4ml of CSR.flush @13:16:39.57 CSR.select :flush @13:16:39.73 CS.seek 4ml @13:16:51.27 delay 5 seconds @13:16:56.30 CSR.select :air @13:16:56.45 CS.seek 10ml @13:17:12.92 CSV.dial CTV @13:17:12.96
CTV.dial CSV @13:17:13.52 CBV.dial :waste1 @13:17:13.55 CS.seek 0ml @13:17:38.96 delay 3 seconds @13:17:40.53
Garaging FlushPuck in Collection Clamp @13:17:40.64 Elbow.reconfigure TightElbow @13:17:40.71 Elbow.seek CC @13:17:41.00 Elbow.reconfigure LooseElbow @13:17:41.12 Forearm.seek CC @13:17:41.98 ATV.dial :waste2 @13:17:42.19
Hand.close @13:17:42.78 AS.reconfigure ASconfig @13:17:42.85 AS.seek :empty @13:17:42.95
CC.seek :open @13:17:47.37 Forearm.seek :retract @13:17:48.49 Elbow.reconfigure TightElbow @13:17:48.57 Elbow.seek :garage @13:17:48.66 ATV.dial :air2 @13:17:49.05 AS.reconfigure ASslow1 @13:17:49.13 AS.seek 1ml @13:17:51.68
Elbow.reconfigure LooseElbow @13:17:51.81 Forearm.reconfigure TightForearm @13:17:51.88 Forearm.seek :garage @13:17:53.05 Hand.open @13:17:53.89 Forearm.reconfigure LooseForearm @13:17:53.96 Forearm.seek :retract @13:17:55.05 Whole Cell Archive sampling 50ml @13:17:55.07 Loading wcr puck from tube 2 into Collection Clamp @13:17:55.90 SC.reconfigure SCtight @13:17:55.98 SC.seek 2 @13:17:58.20 SC.reconfigure SCloose @13:17:58.47 Elbow.reconfigure TightElbow @13:17:58.54 Elbow.seek 2 @13:18:00.74 Forearm.reconfigure TightForearm @13:18:00.82 Forearm.seek 2 + 700 counts @13:18:01.95 SE.reconfigure SEpick @13:18:02.06 SE.seek beamBreak @13:18:05.28 SE.configure SEconfig @13:18:05.48 Elbow.seek 2 @13:18:05.72 Forearm.seek 2 + 300 counts @13:18:06.00 Forearm.reconfigure LooseForearm @13:18:06.07 Hand.close @13:18:06.84 SE.seek :down @13:18:07.10 Forearm.seek :retract @13:18:08.08 Elbow.seek CC @13:18:09.70 Elbow.reconfigure LooseElbow @13:18:09.84 Forearm.reconfigure TightForearm @13:18:09.94 Forearm.seek CC @13:18:09.97 SE.coast @13:18:11.43 CC.seek :closed @13:18:15.76 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.39 @13:18:15.77 Hand.open @13:18:16.65 Forearm.reconfigure LooseForearm @13:18:16.72 Forearm.seek :retract @13:18:17.79 SC.coast @13:18:17.86 collectSample 50ml w/bubble point of 55psi @13:18:17.88 Priming 7ml @13:18:17.90 Intake.select :close @13:18:17.93 Exhaust.select :close @13:18:17.95 SS.hold @13:18:18.22 Calibrating pressure sensors @13:18:18.24 CTV.dial :air @13:18:19.86 SSV.dial :spit,avoiding: :exhaust @13:18:19.89 SS.reconfigure SSfast @13:18:19.98 SS.seek 5ml @13:18:22.99 SSV.dial CBV @13:18:23.03 CBV.dial SSV @13:18:24.71 delay 3.8 seconds @13:18:28.65 SSV.dial :spit @13:18:30.20 SS.seek 0ml @13:18:32.84 SSV.dial CBV @13:18:34.41 CTV.dial :intake @13:18:35.35 SSV.dial CBV @13:18:35.38 CBV.dial SSV @13:18:35.95 Allowing 40s for 7.0ml of remaining 7.0ml @13:18:36.00 Intake.select :open @13:18:38.22 Intake @15.3psia, vacuum from 8.8 to 12.8psi @13:18:38.25 SS.reconfigure SSinhale.with maxSpeed: 150 @13:18:38.34 SS.seek 7ml @13:18:45.12 Stroke completed -- waiting for pressure to equalize ... @13:18:59.15 Intake.select :close @13:18:59.34 SSV.dial :exhaust @13:19:00.24 Exhaust.select :open @13:19:00.39 SS.reconfigure SSexhale @13:19:00.46 SS.seek 0ml @13:19:03.61 Slide::Error in WCR -- Sampler Syringe overCurrent at 1.48ml @13:19:03.66 SS.reconfigure SSconfig @13:19:03.73 SS.seek 0ml @13:19:04.78 Exhaust.select :close @13:19:04.92 Slide::Error in MAIN -- Sampler Syringe overCurrent at 1.47ml @13:19:04.94 Waiting to Resume from Checkpoint "Sampler Syringe overCurrent at 1.47ml" @13:19:04.96 BadNews.email "Sampler Syringe overCurrent at 1.47ml",Subject: "Slide::Error in MAIN" @13:21:10.78 delay 5 seconds @13:21:15.82 ATV.dial ASV @13:21:16.39 Session Begun @13:21:16.88 ASV.dial :SPEdelivery @13:21:16.93 AS.seek 0.02ml @13:21:25.58 -> showlog 100 @13:24:34.35 delay 5 seconds @13:24:39.38 ATV.dial :air2 @13:24:40.49 AS.seek 0.52ml @13:26:21.38 delay 5 seconds @13:26:26.41 ATV.dial ASV @13:26:27.44 ASV.dial :wasteR @13:26:27.95 AS.seek 0.3ml @13:27:12.53 delay 20 seconds @13:27:32.57 AS.seek 0.07ml @13:28:19.13 delay 20 seconds @13:28:39.16 ATV.dial :waste2 @13:28:40.06 AS.reconfigure ASconfig @13:28:40.14 AS.seek :empty @13:28:44.54 Elution @13:28:44.55 ATV.dial :air1 @13:28:45.76 AS.reconfigure ASslow1 @13:28:46.38 AS.seek 0.049ml @13:28:56.83 delay 3 seconds @13:28:59.86 ATV.dial :ElutionWater @13:29:00.57 AS.seek 0.109ml @13:29:12.97 delay 3 seconds @13:29:16.00 ATV.dial ASV,via: :air2 @13:29:18.17 ASV.dial :SPEelution @13:29:18.81 AFV.select :carrierSolution @13:29:19.00 AS.reconfigure ASnorm @13:29:19.09 AS.seek 0.759ml @13:29:52.16 delay 3 seconds @13:29:55.19 AFV.select :holdingCoil @13:29:55.37 AS.reconfigure ASslow1 @13:29:55.45 AS.seek 0.474ml @13:30:53.08 delay 5 seconds @13:30:58.18 AS.reconfigure ASslow2 @13:30:58.26 AS.seek 0.439ml @13:31:33.67 delay 1:00 @13:32:33.70 pushing eluate to holding coil @13:32:33.81 AS.seek 0.279ml @13:35:15.05 delay 30 seconds @13:35:45.08 ATV.dial :waste,via: :air3 @13:35:46.85 AS.reconfigure ASconfig @13:35:46.93 AS.seek :empty @13:36:03.39 Parking Bleach in LysateSource line and SPEcolumn @13:36:03.40 ATV.dial :air3 @13:36:04.31 AS.reconfigure ASslow1 @13:36:04.39 AS.seek 0.029ml @13:36:10.75 ATV.dial :ElutionWater @13:36:11.42 AS.seek 0.059ml @13:36:17.75 ATV.dial :air3 @13:36:18.42 AS.seek 0.069ml @13:36:20.72 delay 2 seconds @13:36:22.74 ATV.dial :bleach @13:36:23.24 AS.seek 0.769ml @13:38:44.39 delay 3 seconds @13:38:47.42 ATV.dial :air3 @13:38:48.02 delay 3 seconds @13:38:51.11 AS.seek 0.794ml @13:38:56.46 delay 3 seconds @13:38:59.49 ATV.dial :bleach @13:39:00.17 AS.seek 0.779ml @13:39:03.50 delay 3 seconds @13:39:06.53 ATV.dial :AIV @13:39:07.85 AS.reconfigure ASconfig @13:39:07.93 AS.seek 0.015ml @13:39:52.18 ATV.dial :waste @13:39:53.72 AS.seek :empty @13:39:54.96 ATV.dial :air3 @13:39:55.67 AS.reconfigure ASslow1 @13:39:55.76 AS.seek 0.009ml @13:39:58.28 delay 2 seconds @13:40:00.31 ATV.dial :bleach @13:40:00.81 AS.seek 0.579ml @13:41:55.74 delay 3 seconds @13:41:58.77 ATV.dial :air3 @13:41:59.38 delay 3 seconds @13:42:02.47 AS.seek 0.604ml @13:42:07.81 delay 3 seconds @13:42:10.84 ATV.dial :bleach @13:42:11.49 AS.seek 0.589ml @13:42:14.81 delay 3 seconds @13:42:17.84 ATV.dial :ASV @13:42:18.92 ASV.dial :wasteR @13:42:19.56 AS.seek 0ml @13:44:18.36 delay 1:00 @13:45:18.39 AFV.select :carrierSolution @13:45:18.52 delay 2 seconds @13:45:20.55 AS.reconfigure ASconfig @13:45:20.63 AS.seek 1ml @13:46:18.44 delay 1 second @13:46:19.46 AFV.select :holdingCoil @13:46:19.59 delay 1 second @13:46:20.62 ATV.dial ASV @13:46:20.63 ASV.dial :wasteR @13:46:20.69 AS.reconfigure ASslow1 @13:46:20.77 AS.seek 0ml @13:49:42.21 delay 20 seconds @13:50:02.24 ATV.dial :air3 @13:50:03.83 AS.seek 0.59ml @13:52:02.81 delay 3 seconds @13:52:05.84 ATV.dial :ASV @13:52:07.31 ASV.dial :wasteR @13:52:07.43 AS.seek 0.09ml @13:52:27.45 -> progress @13:53:48.25 delay 30 seconds @13:54:18.28 ATV.dial :extSrc @13:54:19.63 delay 3 seconds @13:54:22.66 ATV.dial :ElutionWater @13:54:23.83 AS.seek 0.12ml @13:54:30.27 ATV.dial :air3 @13:54:30.93 AS.seek 0.13ml @13:54:33.30 delay 2 seconds @13:54:35.32 ATV.dial :bleach @13:54:35.82 AS.seek 0.23ml @13:54:56.25 delay 3 seconds @13:54:59.28 ATV.dial :air3 @13:54:59.86 delay 3 seconds @13:55:02.95 AS.seek 0.255ml @13:55:08.25 delay 3 seconds @13:55:11.28 ATV.dial :bleach @13:55:11.95 AS.seek 0.24ml @13:55:15.31 delay 3 seconds @13:55:18.34 ATV.dial :ASV @13:55:19.42 ASV.dial :SPEdelivery @13:55:19.83 AFV.select :carrierSolution @13:55:19.97 delay 2 seconds @13:55:22.00 AS.reconfigure ASconfig @13:55:22.08 AS.seek 0.5ml @13:55:35.72 -> exhaust @13:55:35.75 NameError in bkha#2 -- undefined local variable or method `exhaust' for # @13:55:37.36 delay 1 second @13:55:38.39 AFV.select :holdingCoil @13:55:38.58 AS.reconfigure ASslow1 @13:55:38.67 AS.seek 0.17ml @13:55:54.54 -> SSV @13:56:03.92 -> exhaust @13:56:03.93 NameError in bkha#2 -- undefined local variable or method `exhaust' for # @13:56:13.67 -> exhaust.open @13:56:13.68 NameError in bkha#2 -- undefined local variable or method `exhaust' for # @13:56:29.21 -> EXhaust @13:56:29.22 NameError in bkha#2 -- uninitialized constant EXhaust @13:56:32.17 -> Exhaust @13:56:45.37 SPE.hold 2:46:40,95C @13:56:45.44 delay 3:00 @13:56:45.80 -> Exhaust.open @13:56:45.82 Exhaust.select :open @13:56:56.21 -> SSV @13:58:06.29 -> SSV.push 0.5 @13:58:06.31 NoMethodError in bkha#2 -- undefined method `push' for SSV:Shaft @13:58:14.73 -> SS @13:58:23.29 -> SS.push 0.1 @13:58:23.41 SS.seek 3.13ml @13:58:42.01 -> MainThread.recover @13:58:42.04
Recovering Thread after Sampler Syringe overCurrent at 1.47ml at 13:19:03 @13:58:42.10 SS.seek 0ml @13:58:44.89 Exhaust.select :close @13:58:45.03 Sampling 50.0ml @13:58:45.05 Intake.select :close @13:58:45.08 Exhaust.select :close @13:58:45.12 SS.hold @13:58:45.39 CTV.dial :intake @13:58:45.41 SSV.dial CBV @13:58:45.45
CBV.dial SSV @13:58:46.43 Allowing 130s for 25.0ml of remaining 50.0ml @13:58:46.45 Intake.select :open @13:58:48.69 Intake @15.5psia, vacuum from 9.0 to 13.0psi @13:58:48.72 SS.reconfigure SSinhale.with maxSpeed: 150 @13:58:48.82 SS.seek 25ml @13:58:54.82 Vacuum too high -- waiting up to 40s @13:59:09.36 Intake @15.3psia, vacuum from 8.8 to 12.8psi @13:59:09.39 SS.reconfigure SSinhale.with maxSpeed: 135 @13:59:09.50 SS.seek 25ml @13:59:14.41 Vacuum too high -- waiting up to 40s @13:59:31.82 Intake @15.5psia, vacuum from 9.0 to 13.0psi @13:59:31.85 SS.reconfigure SSinhale.with maxSpeed: 122 @13:59:31.96 SS.seek 25ml @13:59:37.26 Vacuum too high -- waiting up to 40s @13:59:45.47 AFV.select :carrierSolution @13:59:45.66 delay 2 seconds @13:59:47.76 AS.reconfigure ASconfig @13:59:47.84 AS.seek 1ml @13:59:55.99
SS.reconfigure SSinhale.with maxSpeed: 110 @13:59:56.08 SS.seek 25ml @14:00:00.33 Stroke completed -- waiting for pressure to equalize ... @14:00:14.94 Intake.select :close @14:00:15.14 SSV.dial :exhaust @14:00:16.11 Exhaust.select :open @14:00:16.27 SS.reconfigure SSexhale @14:00:16.34 SS.seek 0ml @14:00:25.93 Exhaust.select :close @14:00:26.08 SSV.dial CBV @14:00:26.12
CBV.dial SSV @14:00:27.11 Allowing 130s for 25.0ml of remaining 25.0ml @14:00:27.13 Intake.select :open @14:00:29.36 Intake @15.5psia, vacuum from 9.0 to 13.0psi @14:00:29.39 SS.reconfigure SSinhale.with maxSpeed: 110 @14:00:29.49 SS.seek 25ml @14:00:34.01 Vacuum too high -- waiting up to 40s @14:00:35.88 delay 1 second @14:00:36.90 AFV.select :holdingCoil @14:00:37.09 delay 1 second @14:00:38.18 ASV.dial :wasteD @14:00:39.35 AS.seek 0ml @14:00:45.41
Intake @15.5psia, vacuum from 9.0 to 13.0psi @14:00:45.44 SS.reconfigure SSinhale.with maxSpeed: 99 @14:00:45.55 SS.seek 25ml @14:00:49.81 Vacuum too high -- waiting up to 40s @14:01:02.02 Intake @15.4psia, vacuum from 8.9 to 12.9psi @14:01:02.05 SS.reconfigure SSinhale.with maxSpeed: 89 @14:01:02.16 SS.seek 25ml @14:01:07.10 Vacuum too high -- waiting up to 40s @14:01:21.11 Intake @15.5psia, vacuum from 9.0 to 13.0psi @14:01:21.13 SS.reconfigure SSinhale.with maxSpeed: 80 @14:01:21.24 SS.seek 25ml @14:01:27.20 Vacuum too high -- waiting up to 40s @14:01:37.17 Priming Eluate @14:01:37.18 AFV.select :carrierSolution @14:01:37.40 delay 2 seconds @14:01:39.52 AS.seek 0.5ml @14:01:42.17
Intake @15.5psia, vacuum from 9.0 to 13.0psi @14:01:42.20 SS.reconfigure SSinhale.with maxSpeed: 72 @14:01:42.31 SS.seek 25ml @14:01:48.12 Stroke completed -- waiting for pressure to equalize ... @14:02:06.63 Intake.select :close @14:02:06.83 SSV.dial :exhaust @14:02:07.79 Exhaust.select :open @14:02:07.96 SS.reconfigure SSexhale @14:02:08.04 SS.seek 0ml @14:02:08.57 delay 1 second @14:02:09.60 AFV.select :holdingCoil @14:02:09.74 ATV.dial ASV @14:02:09.76 ASV.dial :SPErecovery @14:02:10.42 Swish #1 of 2 @14:02:10.53 AS.reconfigure ASslow0 @14:02:10.61 AS.seek 0.6ml @14:02:17.75
Exhaust.select :close @14:02:17.89 Sampled 50.0ml @14:02:17.92 Drying puck before fixing for 4:00 @14:02:17.95 CBV.dial :waste2b @14:02:18.93 delay 1:00 @14:02:21.04 AS.seek 0.5ml @14:02:31.45 Swish #2 of 2 @14:02:31.57 AS.seek 0.6ml @14:02:42.09 AS.seek 0.5ml @14:02:52.49 delay 3 seconds @14:02:55.59 AS.reconfigure ASslow1 @14:02:55.67 AS.seek 0.684ml @14:03:18.95
CSV.dial :air @14:03:19.51 CS.reconfigure CSfast @14:03:19.61 CS.seek 10ml @14:03:33.14 delay 3 seconds @14:03:36.17 Eluate primed @14:03:36.18 ATV.dial :waste2 @14:03:36.86 AS.reconfigure ASnorm @14:03:36.94 AS.seek 0ml @14:03:48.20
Pumping wcr puck from tube 2 up to 55psi... @14:03:48.22 CBV.dial SSV @14:03:49.79 CBV.dial between CSV and waste2b @14:03:51.51 CSV.dial between air and CTV @14:03:52.02 CS.seek 7.137ml @14:04:00.81 CTV.dial CSV,avoiding: :air @14:04:00.85
CSV.dial CTV @14:04:01.88 CS.reconfigure CSpressurize @14:04:01.96 CS.seek 0ml @14:04:11.78 SPE.coast @14:04:11.90 ASV.dial :SPErecovery @14:04:11.93 Setting up PCR for OM -- writing /var/log/esp/OM20may0114h.pcr @14:04:11.96 Building PCR Slug from ASV with MM3 mix, PP5 primer @14:04:12.00 Priming with carrier @14:04:12.04 ATV.dial ARV @14:04:13.07 ARV.dial :carrier @14:04:13.47 AS.seek 0.5ml @14:04:18.35
CBV.dial :waste2b @14:04:18.76 CS.reconfigure CSconfig @14:04:18.84 CS.seek :empty @14:04:23.23 delay 1:00 @14:04:39.06 AFV.select :carrierSolution @14:04:39.20 delay 2 seconds @14:04:41.23 AS.reconfigure ASconfig @14:04:41.32 AS.seek 1ml @14:05:10.38 delay 1 second @14:05:11.41 AFV.select :holdingCoil @14:05:11.54 delay 1 second @14:05:12.57 ATV.dial :waste @14:05:14.12 AS.reconfigure ASnorm @14:05:14.21 AS.seek :empty @14:05:23.26
CTV.dial :air,avoiding: :intake @14:05:24.88 Fix #1 of 2 (with rnal) for 2:00 @14:05:24.90 CSV.dial CSR @14:05:25.55 Pulling 2ml of CSR.rnal @14:05:25.57 CSR.select :rnal @14:05:25.74 CS.seek 2ml @14:05:32.02 delay 5 seconds @14:05:37.05 CSR.select :air @14:05:37.20 CS.reconfigure CSfast @14:05:37.28 CS.seek 10ml @14:05:59.33 Pumping wcr puck from tube 2 up to 55psi... @14:05:59.35 CBV.dial SSV @14:06:00.88 CBV.dial between CSV and waste2b @14:06:02.09 CSV.dial between air and CTV @14:06:02.87 CS.seek 7.073ml @14:06:05.00 AFV.select :carrierSolution @14:06:05.15 delay 2 seconds @14:06:07.18 AS.reconfigure ASconfig @14:06:07.27 AS.seek 0.5ml @14:06:11.62 CTV.dial CSV,avoiding: :air @14:06:11.65
CSV.dial CTV @14:06:13.37 CS.reconfigure CSpressurize @14:06:13.46 CS.seek 0ml @14:06:31.57 delay 2:00 @14:06:36.38 delay 1 second @14:06:37.40 AFV.select :holdingCoil @14:06:37.53 Building Slug @14:06:37.54 ATV.dial :ARV @14:06:39.24 delay 2 seconds @14:06:41.27 ARV.dial :air1 @14:06:41.72 delay 2 seconds @14:06:43.81 AS.reconfigure ASslow2 @14:06:43.90 AS.seek 0.506ml @14:06:50.31 delay 2 seconds @14:06:52.34 ARV.dial :MM3 @14:06:53.46 delay 2 seconds @14:06:55.49 Adding MM3 @14:06:55.62 AS.seek 0.524ml @14:07:14.02 delay 25 seconds @14:07:39.05 ARV.dial :PP5 @14:07:39.47 Adding PP5 @14:07:39.49 delay 2 seconds @14:07:41.58 AS.seek 0.53ml @14:07:47.81 delay 10 seconds @14:07:57.84 ARV.dial :air3 @14:07:58.25 adding ARV air @14:07:58.27 delay 2 seconds @14:08:01.02 AS.seek 0.536ml @14:08:07.30 delay 5 seconds @14:08:12.33 ARV.dial :carrier @14:08:13.64 delay 7 seconds @14:08:20.73 AS.seek 0.594ml @14:08:31.60
CBV.dial :waste2b @14:08:32.00 CS.reconfigure CSconfig @14:08:32.08 CS.seek :empty @14:08:34.24 delay 1:00 @14:09:19.63 delay 5 seconds @14:09:24.66 ATV.dial :ASV @14:09:25.11 delay 2 seconds @14:09:27.21 AS.seek 0.6ml @14:09:33.53 delay 4 seconds @14:09:34.26
CTV.dial :air,avoiding: :intake @14:09:35.89 Fix #2 of 2 (with rnal) for 2:00 @14:09:35.91 CSV.dial CSR @14:09:36.55 Pulling 2ml of CSR.rnal @14:09:36.56 CSR.select :rnal @14:09:36.73 CS.seek 2ml @14:09:37.55 ATV.dial ARV @14:09:38.25 delay 2 seconds @14:09:40.28 ARV.dial :carrier @14:09:40.31 delay 2 seconds @14:09:42.43 AS.seek 0.65ml @14:09:43.05
delay 5 seconds @14:09:48.08 CSR.select :air @14:09:48.23 CS.reconfigure CSfast @14:09:48.31 CS.seek 10ml @14:10:10.24 Pumping wcr puck from tube 2 up to 55psi... @14:10:10.26 CBV.dial SSV @14:10:11.79 CBV.dial between CSV and waste2b @14:10:12.99 CSV.dial between air and CTV @14:10:13.75 CS.seek 7.011ml @14:10:22.56 CTV.dial CSV,avoiding: :air @14:10:22.59
CSV.dial CTV @14:10:24.31 CS.reconfigure CSpressurize @14:10:24.40 CS.seek 0ml @14:10:33.10 delay 4 seconds @14:10:37.13 ATV.dial PCR @14:10:37.77 ASV.dial :waste1 @14:10:38.60 ARV.dial :air1 @14:10:39.03 Positioning Slug by 0.324ml @14:10:39.16 AS.seek 0.326ml @14:10:42.88
delay 2:00 @14:12:42.91 CBV.dial :waste2b @14:12:43.32 CS.reconfigure CSconfig @14:12:43.40 CS.seek :empty @14:12:45.08 delay 1:00 @14:13:45.10 CTV.dial :air,avoiding: :intake @14:13:46.79 Storing wcr puck in Collection Clamp to tube 1 @14:13:47.02 SC.reconfigure SCtight @14:13:47.10 SC.seek 1 @14:13:49.28 SC.reconfigure SCloose @14:13:49.54 Elbow.reconfigure TightElbow @14:13:49.62 Elbow.seek 1 @14:13:51.57 Forearm.reconfigure TightForearm @14:13:51.66 Forearm.seek 1 + 700 counts @14:13:52.73 SE.reconfigure SEpick @14:13:52.84 SE.seek beamBreak @14:13:55.97 SE.configure SEconfig @14:13:56.76 Elbow.reconfigure LimpElbow @14:13:56.85 Forearm.reconfigure LooseForearm @14:13:56.93 Forearm.seek 1 @14:13:57.27 Elbow.hold @14:13:57.38 Elbow.reconfigure TightElbow @14:13:57.52 SE.seek 1.08 Pucks below Top Plate @14:13:58.20 Forearm.force -20 @14:13:58.28 Forearm.seek 1 + 342 counts @14:13:58.65 Forearm.reconfigure TightForearm @14:13:58.73 Forearm.seek 1 + 700 counts @14:13:59.14 SE.seek 2.08 Pucks below Top Plate @14:13:59.78 Forearm.reconfigure LooseForearm @14:13:59.87 Forearm.seek :retract @14:14:00.82 Elbow.seek CC @14:14:02.59 Elbow.reconfigure LooseElbow @14:14:02.73 Forearm.seek CC @14:14:03.80 Hand.close @14:14:04.63 CC.seek :open @14:14:09.19 Forearm.seek :retract @14:14:10.30 Elbow.reconfigure TightElbow @14:14:10.39 Elbow.seek 1 @14:14:12.24 Elbow.reconfigure LimpElbow @14:14:12.33 Forearm.seek 1 @14:14:13.37 Elbow.hold @14:14:13.49 Elbow.reconfigure TightElbow @14:14:13.56 Hand.open @14:14:14.52 Forearm.force -20 @14:14:14.60 Forearm.seek 1 + 349 counts @14:14:14.96 Forearm.reconfigure TightForearm @14:14:15.05 Forearm.seek 1 + 700 counts @14:14:15.51 SE.seek :down @14:14:16.66
Forearm.reconfigure LooseForearm @14:14:16.75 Forearm.seek :retract @14:14:18.30 SE.coast @14:14:18.37
SC.coast @14:14:18.45 Starting finalPurge @14:14:18.46 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:14:18.59 Hand.open @14:14:19.02 Elbow.seek FlushPuck::Garage @14:14:21.08 Elbow.reconfigure LooseElbow @14:14:21.23 Forearm.seek :garage @14:14:22.30 Hand.close @14:14:23.26 Forearm.seek :retract @14:14:24.94 Elbow.reconfigure TightElbow @14:14:25.04 Elbow.seek CC @14:14:27.92 Elbow.reconfigure LooseElbow @14:14:28.06 Forearm.reconfigure TightForearm @14:14:28.14 Forearm.seek CC @14:14:29.53 CC.seek :closed @14:14:33.89 Collection Clamp onSpring/offSpring for 5/31 samples, on/off torque 2.35 @14:14:34.00 Hand.open @14:14:34.83 Forearm.reconfigure LooseForearm @14:14:34.91 Forearm.seek :retract @14:14:35.98 Flushing the Collection Valve::Manifold @14:14:36.17 CSV.dial CSR @14:14:36.77 Pulling 5ml of CSR.flush @14:14:36.78 CSR.select :flush @14:14:36.96 CS.seek 5ml @14:14:50.94 delay 5 seconds @14:14:55.97 CSR.select :air @14:14:56.18 CS.reconfigure CSfast @14:14:56.26 CS.seek 7.5ml @14:15:03.80 CSV.dial :waste @14:15:05.41 CS.reconfigure CSconfig @14:15:05.49 CS.seek 0ml @14:15:25.93 Flushing lower manifold's waste1 port @14:15:26.11 CSV.dial :flush @14:15:26.65 CS.reconfigure CSfast @14:15:26.74 CS.seek 5ml @14:15:40.71 delay 5 seconds @14:15:45.74 CSV.dial :air @14:15:46.40 CS.seek 7ml @14:15:52.77 CSV.dial CTV @14:15:52.81
CTV.dial CSV @14:15:54.52 CBV.dial :waste1 @14:15:55.47 CS.seek 0ml @14:16:05.17 delay 5 seconds @14:16:10.20 ATV.dial ARV @14:16:11.00 ASV.dial :PCRlock @14:16:11.42 delay 5 seconds @14:16:14.65
Flushing lower manifold's waste2b port @14:16:14.83 CSV.dial :flush @14:16:15.76 CS.seek 5ml @14:16:16.45 Running OM Profile60C350 thermocycling @14:16:16.47 PCR.program Profile60C350 @14:16:16.63 Profile60C350 should complete in < 1:46:29 @14:16:16.65 PCR.run 2:18:06.25 @14:16:30.02
delay 5 seconds @14:16:35.04 CSV.dial :air @14:16:35.69 CS.seek 7ml @14:16:41.90 CSV.dial CTV @14:16:41.94
CTV.dial CSV @14:16:42.52 CBV.dial :waste2b @14:16:43.48 CS.seek 0ml @14:17:02.74 Flushing lower manifold's waste1 port @14:17:02.76 CSV.dial CSR @14:17:03.40 Pulling 4ml of CSR.mfbkill @14:17:03.41 CSR.select :mfbkill @14:17:03.59 CS.reconfigure CSconfig @14:17:03.67 CS.seek 4ml @14:17:15.18 delay 5 seconds @14:17:20.21 CSR.select :air @14:17:20.38 CS.reconfigure CSfast @14:17:20.46 CS.seek 10ml @14:17:37.12 CSV.dial CTV @14:17:37.16
CTV.dial CSV @14:17:37.77 CBV.dial :waste1 @14:17:38.72 CS.seek 0ml @14:18:05.80 Flushing lower manifold's waste2b port @14:18:05.82 CSV.dial CSR @14:18:06.48 Pulling 4ml of CSR.mfbkill @14:18:06.49 CSR.select :mfbkill @14:18:06.66 CS.reconfigure CSconfig @14:18:06.74 CS.seek 4ml @14:18:18.43 delay 5 seconds @14:18:23.46 CSR.select :air @14:18:23.65 CS.reconfigure CSfast @14:18:23.73 CS.seek 10ml @14:18:40.34 CSV.dial CTV @14:18:40.38
CTV.dial CSV @14:18:40.98 CBV.dial :waste2b @14:18:41.92 CS.seek 0ml @14:19:09.02 Killing upper manifold @14:19:09.04 CSV.dial CSR @14:19:09.70 Pulling 4ml of CSR.mfbkill @14:19:09.71 CSR.select :mfbkill @14:19:09.89 CS.reconfigure CSconfig @14:19:09.97 CS.seek 4ml @14:19:21.40 delay 5 seconds @14:19:26.43 CSR.select :air @14:19:26.59 CS.reconfigure CSfast @14:19:26.67 CS.seek 10ml @14:19:43.37 CSV.dial CBV @14:19:43.40
CBV.dial CSV @14:19:44.74 CTV.dial :air @14:19:46.36 CS.seek 0ml @14:20:13.43 delay 7 seconds @14:20:20.46 CS.seek 3ml @14:20:29.15 CSV.dial :waste @14:20:29.72 CS.seek 0ml @14:20:38.52 Flushing upper manifold @14:20:38.70 CSV.dial :flush @14:20:39.27 CS.seek 5ml @14:20:53.46 delay 5 seconds @14:20:58.48 CSV.dial :air @14:20:59.12 CS.seek 7ml @14:21:05.40 CSV.dial CBV @14:21:05.44
CBV.dial CSV @14:21:06.74 CTV.dial :air @14:21:06.78 CS.seek 0ml @14:21:25.96 delay 7 seconds @14:21:32.99 CS.seek 3ml @14:21:41.74 CSV.dial :waste @14:21:42.33 CS.seek 0ml @14:21:51.33 CSV.dial :flush @14:21:51.91 CS.seek 3ml @14:22:00.74 delay 5 seconds @14:22:05.77 CSV.dial :air @14:22:07.00 CS.seek 5ml @14:22:13.21 CSV.dial :air @14:22:13.24 CS.seek 0ml @14:22:27.21 delay 7 seconds @14:22:34.24 CS.seek 3ml @14:22:43.09 CSV.dial :waste @14:22:44.04 CS.seek 0ml @14:22:52.90 Flushing lower manifold's waste1 port @14:22:53.10 CSV.dial :flush @14:22:53.68 CS.seek 5ml @14:23:07.68 delay 5 seconds @14:23:12.70 CSV.dial :air @14:23:13.44 CS.seek 7ml @14:23:19.65 CSV.dial CTV @14:23:19.69
CTV.dial CSV @14:23:21.40 CBV.dial :waste1 @14:23:21.98 CS.seek 0ml @14:23:41.09 Flushing lower manifold's waste2b port @14:23:41.27 CSV.dial :flush @14:23:42.21 CS.seek 5ml @14:23:56.12 delay 5 seconds @14:24:01.14 CSV.dial :air @14:24:01.79 CS.seek 7ml @14:24:07.99 CSV.dial CTV @14:24:08.03
CTV.dial CSV @14:24:08.60 CBV.dial :waste2b @14:24:09.54 CS.seek 0ml @14:24:28.78 Garaging FlushPuck in Collection Clamp @14:24:28.90 Elbow.reconfigure TightElbow @14:24:28.98 Elbow.seek CC @14:24:29.24 Elbow.reconfigure LooseElbow @14:24:29.39 Forearm.seek CC @14:24:30.47 Hand.close @14:24:31.30 CC.seek :open @14:24:35.75 Forearm.seek :retract @14:24:36.82 Elbow.reconfigure TightElbow @14:24:36.91 Elbow.seek :garage @14:24:40.08 Elbow.reconfigure LooseElbow @14:24:40.22 Forearm.reconfigure TightForearm @14:24:40.31 Forearm.seek :garage @14:24:41.50 Hand.open @14:24:42.34 Forearm.reconfigure LooseForearm @14:24:42.42 Forearm.seek :retract @14:24:43.47 Completed [coreNegNTCphase] begun 12:23:53 @14:24:43.49 Extending 20invasivedeploy until completion of MFBPCR @15:39:08.97 PCR.off @15:39:10.61 PCR for OM completed -- closed /var/log/esp/OM20may0114h.pcr @15:39:10.72 Cleaning PCR @15:39:10.74 AFV.select :carrierSolution @15:39:10.89 delay 2 seconds @15:39:12.92 AS.reconfigure ASconfig @15:39:13.01 AS.seek 0.5ml @15:39:23.33 delay 1 second @15:39:24.35 AFV.select :holdingCoil @15:39:24.49 ASV.dial :waste1 @15:39:24.91 ATV.dial :air3 @15:39:26.25 AS.reconfigure ASslow1 @15:39:26.33 AS.seek 0.51ml @15:39:28.67 delay 2 seconds @15:39:30.69 ATV.dial :bleach @15:39:31.34 AS.seek 0.81ml @15:40:31.96 delay 3 seconds @15:40:34.99 ATV.dial :air3 @15:40:35.58 delay 3 seconds @15:40:38.67 AS.seek 0.835ml @15:40:44.07 delay 3 seconds @15:40:47.10 ATV.dial :bleach @15:40:47.76 AS.seek 0.82ml @15:40:51.11 delay 3 seconds @15:40:54.14 ARV.dial :waste @15:40:54.78 delay 3 seconds @15:40:57.81 ATV.dial :ARV @15:40:58.74 AS.reconfigure ASconfig @15:40:58.83 AS.seek 0.47ml @15:41:19.26 AFV.select :carrierSolution @15:41:19.40 delay 2 seconds @15:41:21.44 AS.seek 1ml @15:41:39.50 -> exit @15:41:39.52 Session Ended @15:41:52.24 delay 1 second @15:41:53.27 AFV.select :holdingCoil @15:41:53.39 delay 1 second @15:41:54.42 AS.reconfigure ASfast @15:41:54.49 AS.seek 0ml @15:42:21.85 AFV.select :carrierSolution @15:42:21.99 delay 2 seconds @15:42:24.02 AS.reconfigure ASconfig @15:42:24.09 AS.seek 1ml @15:43:21.86 delay 1 second @15:43:22.88 AFV.select :holdingCoil @15:43:23.00 delay 1 second @15:43:24.03 AS.reconfigure ASfast @15:43:24.10 AS.seek 0ml @15:43:51.46 AFV.select :carrierSolution @15:43:51.60 delay 2 seconds @15:43:53.63 AS.reconfigure ASconfig @15:43:53.70 AS.seek 1ml @15:44:51.46 delay 1 second @15:44:52.48 AFV.select :holdingCoil @15:44:52.60 delay 1 second @15:44:53.63 AS.reconfigure ASfast @15:44:53.70 AS.seek 0ml @15:45:21.61 ARV.dial between carrier and air1 @15:45:22.13 ATV.dial :air3 @15:45:23.45 AS.reconfigure ASslow1 @15:45:23.52 AS.seek 0.01ml @15:45:25.85 delay 2 seconds @15:45:27.87 ATV.dial :bleach @15:45:28.51 AS.seek 0.81ml @15:48:09.66 delay 3 seconds @15:48:12.69 ATV.dial :air3 @15:48:13.27 delay 3 seconds @15:48:16.36 AS.seek 0.835ml @15:48:21.69 delay 3 seconds @15:48:24.72 ATV.dial :bleach @15:48:25.37 AS.seek 0.82ml @15:48:28.69 delay 3 seconds @15:48:31.72 ATV.dial PCR @15:48:32.13 AS.reconfigure ASconfig @15:48:32.20 AS.seek :empty @15:49:19.69 PCR.program :bleachProfile @15:49:19.76 PCR program should complete in < 2:45 @15:49:19.77 PCR.run 10:00 @15:52:15.56 PCR.off @15:52:15.60 AFV.select :carrierSolution @15:52:15.72 delay 2 seconds @15:52:17.76 AS.seek 1ml @15:53:15.57 delay 1 second @15:53:16.59 AFV.select :holdingCoil @15:53:16.71 delay 1 second @15:53:17.74 AS.reconfigure ASfast @15:53:17.81 AS.seek 0ml @15:53:45.16 delay 3 seconds @15:53:48.19 AFV.select :carrierSolution @15:53:48.31 delay 2 seconds @15:53:50.34 AS.reconfigure ASconfig @15:53:50.42 AS.seek 1ml @15:54:48.19 delay 1 second @15:54:49.21 AFV.select :holdingCoil @15:54:49.33 delay 1 second @15:54:50.36 AS.reconfigure ASfast @15:54:50.43 AS.seek 0ml @15:55:17.77 delay 3 seconds @15:55:20.80 AFV.select :carrierSolution @15:55:20.92 delay 2 seconds @15:55:22.95 AS.reconfigure ASconfig @15:55:23.03 AS.seek 1ml @15:56:20.78 delay 1 second @15:56:21.80 AFV.select :holdingCoil @15:56:21.92 delay 1 second @15:56:22.95 AS.reconfigure ASfast @15:56:23.02 AS.seek 0ml @15:56:50.36 delay 3 seconds @15:56:53.39 AFV.select :carrierSolution @15:56:53.51 delay 2 seconds @15:56:55.54 AS.reconfigure ASconfig @15:56:55.62 AS.seek 1ml @15:57:53.39 delay 1 second @15:57:54.41 AFV.select :holdingCoil @15:57:54.53 delay 1 second @15:57:55.56 AS.reconfigure ASfast @15:57:55.63 AS.seek 0ml @15:58:22.99 delay 3 seconds @15:58:26.02 AFV.select :carrierSolution @15:58:26.14 delay 2 seconds @15:58:28.17 AS.reconfigure ASconfig @15:58:28.24 AS.seek 1ml @15:59:26.02 delay 1 second @15:59:27.04 AFV.select :holdingCoil @15:59:27.16 delay 1 second @15:59:28.19 AS.reconfigure ASnorm @15:59:28.26 AS.seek 0ml @16:00:18.99 Setting up PCR for ML -- writing /var/log/esp/ML20may0116h.pcr @16:00:19.01 Building PCR Slug from ASV with MM2 mix, PP4 primer @16:00:19.05 Priming with carrier @16:00:19.07 ATV.dial ARV @16:00:19.82 ARV.dial :carrier @16:00:20.20 AS.seek 0.5ml @16:00:45.75 AFV.select :carrierSolution @16:00:45.88 delay 2 seconds @16:00:47.91 AS.reconfigure ASconfig @16:00:47.99 AS.seek 1ml @16:01:17.03 delay 1 second @16:01:18.05 AFV.select :holdingCoil @16:01:18.17 delay 1 second @16:01:19.20 ATV.dial :waste @16:01:20.88 AS.reconfigure ASnorm @16:01:20.96 AS.seek :empty @16:02:11.70 AFV.select :carrierSolution @16:02:11.84 delay 2 seconds @16:02:13.87 AS.reconfigure ASconfig @16:02:13.94 AS.seek 0.5ml @16:02:43.08 delay 1 second @16:02:44.10 AFV.select :holdingCoil @16:02:44.22 Building Slug @16:02:44.23 ATV.dial :ARV @16:02:45.91 delay 2 seconds @16:02:47.94 ARV.dial :air1 @16:02:48.94 delay 2 seconds @16:02:51.03 AS.reconfigure ASslow2 @16:02:51.10 AS.seek 0.506ml @16:02:57.53 delay 2 seconds @16:02:59.56 ARV.dial :MM2 @16:03:01.07 delay 2 seconds @16:03:03.10 Adding MM2 @16:03:03.20 AS.seek 0.524ml @16:03:21.74 delay 25 seconds @16:03:46.77 ARV.dial :PP4 @16:03:47.19 Adding PP4 @16:03:47.21 delay 2 seconds @16:03:49.30 AS.seek 0.53ml @16:03:55.60 delay 10 seconds @16:04:05.63 ARV.dial :air3 @16:04:06.05 adding ARV air @16:04:06.07 delay 2 seconds @16:04:08.16 AS.seek 0.536ml @16:04:14.68 delay 5 seconds @16:04:19.71 ARV.dial :carrier @16:04:21.00 delay 7 seconds @16:04:28.09 AS.seek 0.594ml @16:05:26.79 delay 5 seconds @16:05:31.82 ATV.dial :ASV @16:05:32.22 delay 2 seconds @16:05:34.31 AS.seek 0.6ml @16:05:40.52 delay 4 seconds @16:05:44.55 ATV.dial ARV @16:05:45.13 delay 2 seconds @16:05:47.16 ARV.dial :carrier @16:05:47.18 delay 2 seconds @16:05:49.28 AS.seek 0.65ml @16:06:39.79 delay 4 seconds @16:06:43.82 ATV.dial PCR @16:06:44.46 ASV.dial :waste1 @16:06:44.48 ARV.dial :air1 @16:06:44.92 Positioning Slug by 0.324ml @16:06:45.03 AS.seek 0.326ml @16:12:11.43 delay 5 seconds @16:12:16.46 ATV.dial ARV @16:12:17.25 ASV.dial :PCRlock @16:12:17.68 delay 5 seconds @16:12:22.71 Running ML Profile60C350 thermocycling @16:12:22.73 PCR.program Profile60C350 @16:12:22.87 Profile60C350 should complete in < 1:46:29 @16:12:22.89 PCR.run 2:18:06.25 @17:35:22.89 PCR.off @17:35:24.52 PCR for ML completed -- closed /var/log/esp/ML20may0116h.pcr @17:35:24.61 Cleaning PCR @17:35:24.63 AFV.select :carrierSolution @17:35:24.76 delay 2 seconds @17:35:26.79 AS.reconfigure ASconfig @17:35:26.86 AS.seek 0.5ml @17:35:37.16 delay 1 second @17:35:38.19 AFV.select :holdingCoil @17:35:38.30 ASV.dial :waste1 @17:35:38.71 ATV.dial :air3 @17:35:40.03 AS.reconfigure ASslow1 @17:35:40.10 AS.seek 0.51ml @17:35:42.41 delay 2 seconds @17:35:44.44 ATV.dial :bleach @17:35:45.08 AS.seek 0.81ml @17:36:45.69 delay 3 seconds @17:36:48.72 ATV.dial :air3 @17:36:49.29 delay 3 seconds @17:36:52.38 AS.seek 0.835ml @17:36:57.69 delay 3 seconds @17:37:00.72 ATV.dial :bleach @17:37:01.36 AS.seek 0.82ml @17:37:04.71 delay 3 seconds @17:37:07.73 ARV.dial :waste @17:37:08.36 delay 3 seconds @17:37:11.39 ATV.dial :ARV @17:37:12.30 AS.reconfigure ASconfig @17:37:12.37 AS.seek 0.47ml @17:37:32.79 AFV.select :carrierSolution @17:37:32.93 delay 2 seconds @17:37:34.96 AS.seek 1ml @17:38:05.71 delay 1 second @17:38:06.73 AFV.select :holdingCoil @17:38:06.85 delay 1 second @17:38:07.88 AS.reconfigure ASfast @17:38:07.95 AS.seek 0ml @17:38:35.28 AFV.select :carrierSolution @17:38:35.42 delay 2 seconds @17:38:37.45 AS.reconfigure ASconfig @17:38:37.52 AS.seek 1ml @17:39:35.30 delay 1 second @17:39:36.32 AFV.select :holdingCoil @17:39:36.44 delay 1 second @17:39:37.47 AS.reconfigure ASfast @17:39:37.54 AS.seek 0ml @17:40:04.89 AFV.select :carrierSolution @17:40:05.03 delay 2 seconds @17:40:07.06 AS.reconfigure ASconfig @17:40:07.13 AS.seek 1ml @17:41:04.90 delay 1 second @17:41:05.92 AFV.select :holdingCoil @17:41:06.04 delay 1 second @17:41:07.07 AS.reconfigure ASfast @17:41:07.14 AS.seek 0ml @17:41:35.07 ARV.dial between carrier and air1 @17:41:35.58 ATV.dial :air3 @17:41:36.90 AS.reconfigure ASslow1 @17:41:36.97 AS.seek 0.01ml @17:41:39.35 delay 2 seconds @17:41:41.37 ATV.dial :bleach @17:41:42.01 AS.seek 0.81ml @17:44:23.15 delay 3 seconds @17:44:26.18 ATV.dial :air3 @17:44:26.76 delay 3 seconds @17:44:29.85 AS.seek 0.835ml @17:44:35.16 delay 3 seconds @17:44:38.19 ATV.dial :bleach @17:44:38.84 AS.seek 0.82ml @17:44:42.19 delay 3 seconds @17:44:45.22 ATV.dial PCR @17:44:45.63 AS.reconfigure ASconfig @17:44:45.70 AS.seek :empty @17:45:33.19 PCR.program :bleachProfile @17:45:33.25 PCR program should complete in < 2:45 @17:45:33.27 PCR.run 10:00 @17:48:29.05 PCR.off @17:48:29.09 AFV.select :carrierSolution @17:48:29.21 delay 2 seconds @17:48:31.25 AS.seek 1ml @17:49:29.10 delay 1 second @17:49:30.12 AFV.select :holdingCoil @17:49:30.24 delay 1 second @17:49:31.27 AS.reconfigure ASfast @17:49:31.34 AS.seek 0ml @17:49:58.72 delay 3 seconds @17:50:01.75 AFV.select :carrierSolution @17:50:01.87 delay 2 seconds @17:50:03.90 AS.reconfigure ASconfig @17:50:03.99 AS.seek 1ml @17:51:01.77 delay 1 second @17:51:02.79 AFV.select :holdingCoil @17:51:02.91 delay 1 second @17:51:03.94 AS.reconfigure ASfast @17:51:04.01 AS.seek 0ml @17:51:31.38 delay 3 seconds @17:51:34.41 AFV.select :carrierSolution @17:51:34.53 delay 2 seconds @17:51:36.56 AS.reconfigure ASconfig @17:51:36.64 AS.seek 1ml @17:52:34.41 delay 1 second @17:52:35.43 AFV.select :holdingCoil @17:52:35.55 delay 1 second @17:52:36.58 AS.reconfigure ASfast @17:52:36.65 AS.seek 0ml @17:53:04.02 delay 3 seconds @17:53:07.05 AFV.select :carrierSolution @17:53:07.17 delay 2 seconds @17:53:09.20 AS.reconfigure ASconfig @17:53:09.27 AS.seek 1ml @17:54:07.08 delay 1 second @17:54:08.10 AFV.select :holdingCoil @17:54:08.22 delay 1 second @17:54:09.25 AS.reconfigure ASfast @17:54:09.32 AS.seek 0ml @17:54:36.68 delay 3 seconds @17:54:39.71 AFV.select :carrierSolution @17:54:39.83 delay 2 seconds @17:54:41.86 AS.reconfigure ASconfig @17:54:41.94 AS.seek 1ml @17:55:39.76 delay 1 second @17:55:40.78 AFV.select :holdingCoil @17:55:40.90 delay 1 second @17:55:41.93 AS.reconfigure ASnorm @17:55:42.00 AS.seek 0ml @17:56:32.74 Setting up PCR for CG -- writing /var/log/esp/CG20may0117h.pcr @17:56:32.76 Building PCR Slug from ASV with MM1 mix, PP2 primer @17:56:32.79 Priming with carrier @17:56:32.81 ATV.dial ARV @17:56:33.55 ARV.dial :carrier @17:56:33.94 AS.seek 0.5ml @17:56:59.50 AFV.select :carrierSolution @17:56:59.64 delay 2 seconds @17:57:01.67 AS.reconfigure ASconfig @17:57:01.74 AS.seek 1ml @17:57:30.79 delay 1 second @17:57:31.81 AFV.select :holdingCoil @17:57:31.93 delay 1 second @17:57:32.96 ATV.dial :waste @17:57:34.63 AS.reconfigure ASnorm @17:57:34.70 AS.seek :empty @17:58:25.45 AFV.select :carrierSolution @17:58:25.59 delay 2 seconds @17:58:27.62 AS.reconfigure ASconfig @17:58:27.69 AS.seek 0.5ml @17:58:56.82 delay 1 second @17:58:57.84 AFV.select :holdingCoil @17:58:57.96 Building Slug @17:58:57.97 ATV.dial :ARV @17:58:59.65 delay 2 seconds @17:59:01.68 ARV.dial :air1 @17:59:02.11 delay 2 seconds @17:59:04.78 AS.reconfigure ASslow2 @17:59:04.85 AS.seek 0.506ml @17:59:11.10 delay 2 seconds @17:59:13.13 ARV.dial :MM1 @17:59:13.76 delay 2 seconds @17:59:15.79 Adding MM1 @17:59:15.88 AS.seek 0.524ml @17:59:34.33 delay 25 seconds @17:59:59.36 ARV.dial :PP2 @17:59:59.77 Adding PP2 @17:59:59.79 delay 2 seconds @18:00:01.88 AS.seek 0.53ml @18:00:08.32 delay 10 seconds @18:00:18.35 ARV.dial :air2 @18:00:18.76 adding ARV air @18:00:18.78 delay 2 seconds @18:00:20.87 AS.seek 0.536ml @18:00:27.15 delay 5 seconds @18:00:32.18 ARV.dial :carrier @18:00:33.47 delay 7 seconds @18:00:40.56 AS.seek 0.594ml @18:01:39.22 delay 5 seconds @18:01:44.25 ATV.dial :ASV @18:01:44.66 delay 2 seconds @18:01:46.75 AS.seek 0.6ml @18:01:53.07 delay 4 seconds @18:01:57.10 ATV.dial ARV @18:01:57.66 delay 2 seconds @18:01:59.69 ARV.dial :carrier @18:01:59.71 delay 2 seconds @18:02:01.80 AS.seek 0.65ml @18:02:52.27 delay 4 seconds @18:02:56.30 ATV.dial PCR @18:02:56.94 ASV.dial :waste1 @18:02:56.96 ARV.dial :air1 @18:02:57.41 Positioning Slug by 0.324ml @18:02:57.52 AS.seek 0.326ml @18:08:23.66 delay 5 seconds @18:08:28.69 ATV.dial ARV @18:08:29.49 ASV.dial :PCRlock @18:08:29.91 delay 5 seconds @18:08:34.94 Running CG Profile60C300 thermocycling @18:08:34.96 PCR.program Profile60C300 @18:08:35.08 Profile60C300 should complete in < 1:40:53 @18:08:35.10 PCR.run 2:11:06.25 @19:30:16.62 PCR.off @19:30:17.21 PCR for CG completed -- closed /var/log/esp/CG20may0117h.pcr @19:30:17.30 Cleaning PCR @19:30:17.32 AFV.select :carrierSolution @19:30:17.45 delay 2 seconds @19:30:19.48 AS.reconfigure ASconfig @19:30:19.55 AS.seek 0.5ml @19:30:29.90 delay 1 second @19:30:30.92 AFV.select :holdingCoil @19:30:31.04 ASV.dial :waste1 @19:30:31.46 ATV.dial :air3 @19:30:32.78 AS.reconfigure ASslow1 @19:30:32.85 AS.seek 0.51ml @19:30:35.14 delay 2 seconds @19:30:37.16 ATV.dial :bleach @19:30:37.80 AS.seek 0.81ml @19:31:38.41 delay 3 seconds @19:31:41.44 ATV.dial :air3 @19:31:42.01 delay 3 seconds @19:31:45.10 AS.seek 0.835ml @19:31:50.43 delay 3 seconds @19:31:53.46 ATV.dial :bleach @19:31:54.09 AS.seek 0.82ml @19:31:57.43 delay 3 seconds @19:32:00.46 ARV.dial :waste @19:32:01.09 delay 3 seconds @19:32:04.12 ATV.dial :ARV @19:32:05.04 AS.reconfigure ASconfig @19:32:05.12 AS.seek 0.47ml @19:32:25.54 AFV.select :carrierSolution @19:32:25.68 delay 2 seconds @19:32:27.72 AS.seek 1ml @19:32:58.49 delay 1 second @19:32:59.51 AFV.select :holdingCoil @19:32:59.64 delay 1 second @19:33:00.67 AS.reconfigure ASfast @19:33:00.74 AS.seek 0ml @19:33:28.13 AFV.select :carrierSolution @19:33:28.26 delay 2 seconds @19:33:30.29 AS.reconfigure ASconfig @19:33:30.36 AS.seek 1ml @19:34:28.12 delay 1 second @19:34:29.14 AFV.select :holdingCoil @19:34:29.26 delay 1 second @19:34:30.29 AS.reconfigure ASfast @19:34:30.36 AS.seek 0ml @19:34:57.70 AFV.select :carrierSolution @19:34:57.84 delay 2 seconds @19:34:59.87 AS.reconfigure ASconfig @19:34:59.94 AS.seek 1ml @19:35:57.70 delay 1 second @19:35:58.72 AFV.select :holdingCoil @19:35:58.84 delay 1 second @19:35:59.87 AS.reconfigure ASfast @19:35:59.94 AS.seek 0ml @19:36:27.30 ARV.dial between carrier and air1 @19:36:27.80 ATV.dial :air3 @19:36:29.13 AS.reconfigure ASslow1 @19:36:29.21 AS.seek 0.01ml @19:36:31.52 delay 2 seconds @19:36:33.54 ATV.dial :bleach @19:36:34.19 AS.seek 0.81ml @19:39:15.35 delay 3 seconds @19:39:18.38 ATV.dial :air3 @19:39:18.96 delay 3 seconds @19:39:22.05 AS.seek 0.835ml @19:39:27.35 delay 3 seconds @19:39:30.38 ATV.dial :bleach @19:39:31.02 AS.seek 0.82ml @19:39:34.34 delay 3 seconds @19:39:37.37 ATV.dial PCR @19:39:37.77 AS.reconfigure ASconfig @19:39:37.84 AS.seek :empty @19:40:25.32 PCR.program :bleachProfile @19:40:25.38 PCR program should complete in < 2:45 @19:40:25.40 PCR.run 10:00 @19:43:21.74 PCR.off @19:43:21.78 AFV.select :carrierSolution @19:43:21.90 delay 2 seconds @19:43:23.94 AS.seek 1ml @19:44:21.79 delay 1 second @19:44:22.81 AFV.select :holdingCoil @19:44:22.93 delay 1 second @19:44:23.96 AS.reconfigure ASfast @19:44:24.03 AS.seek 0ml @19:44:51.40 delay 3 seconds @19:44:54.43 AFV.select :carrierSolution @19:44:54.55 delay 2 seconds @19:44:56.58 AS.reconfigure ASconfig @19:44:56.66 AS.seek 1ml @19:45:54.43 delay 1 second @19:45:55.45 AFV.select :holdingCoil @19:45:55.57 delay 1 second @19:45:56.60 AS.reconfigure ASfast @19:45:56.67 AS.seek 0ml @19:46:24.02 delay 3 seconds @19:46:27.05 AFV.select :carrierSolution @19:46:27.17 delay 2 seconds @19:46:29.20 AS.reconfigure ASconfig @19:46:29.27 AS.seek 1ml @19:47:27.07 delay 1 second @19:47:28.09 AFV.select :holdingCoil @19:47:28.21 delay 1 second @19:47:29.24 AS.reconfigure ASfast @19:47:29.31 AS.seek 0ml @19:47:56.66 delay 3 seconds @19:47:59.69 AFV.select :carrierSolution @19:47:59.81 delay 2 seconds @19:48:01.84 AS.reconfigure ASconfig @19:48:01.92 AS.seek 1ml @19:48:59.73 delay 1 second @19:49:00.75 AFV.select :holdingCoil @19:49:00.87 delay 1 second @19:49:01.90 AS.reconfigure ASfast @19:49:01.97 AS.seek 0ml @19:49:29.34 delay 3 seconds @19:49:32.37 AFV.select :carrierSolution @19:49:32.49 delay 2 seconds @19:49:34.52 AS.reconfigure ASconfig @19:49:34.60 AS.seek 1ml @19:50:32.43 delay 1 second @19:50:33.45 AFV.select :holdingCoil @19:50:33.57 delay 1 second @19:50:34.60 AS.reconfigure ASnorm @19:50:34.67 AS.seek 0ml @19:51:25.43 Setting up PCR for NM -- writing /var/log/esp/NM20may0119h.pcr @19:51:25.45 Building PCR Slug from ASV with MM1 mix, PP1 primer @19:51:25.48 Priming with carrier @19:51:25.50 ATV.dial ARV @19:51:26.15 ARV.dial :carrier @19:51:26.52 AS.seek 0.5ml @19:51:52.09 AFV.select :carrierSolution @19:51:52.22 delay 2 seconds @19:51:54.25 AS.reconfigure ASconfig @19:51:54.33 AS.seek 1ml @19:52:23.38 delay 1 second @19:52:24.40 AFV.select :holdingCoil @19:52:24.52 delay 1 second @19:52:25.55 ATV.dial :waste @19:52:27.24 AS.reconfigure ASnorm @19:52:27.32 AS.seek :empty @19:53:18.06 AFV.select :carrierSolution @19:53:18.20 delay 2 seconds @19:53:20.23 AS.reconfigure ASconfig @19:53:20.30 AS.seek 0.5ml @19:53:49.37 delay 1 second @19:53:50.39 AFV.select :holdingCoil @19:53:50.51 Building Slug @19:53:50.53 ATV.dial :ARV @19:53:52.20 delay 2 seconds @19:53:54.23 ARV.dial :air1 @19:53:54.64 delay 2 seconds @19:53:56.72 AS.reconfigure ASslow2 @19:53:56.80 AS.seek 0.506ml @19:54:02.98 delay 2 seconds @19:54:05.00 ARV.dial :MM1 @19:54:05.62 delay 2 seconds @19:54:07.65 Adding MM1 @19:54:07.81 AS.seek 0.524ml @19:54:26.12 delay 25 seconds @19:54:51.15 ARV.dial :PP1 @19:54:51.56 Adding PP1 @19:54:51.58 delay 2 seconds @19:54:53.68 AS.seek 0.53ml @19:55:00.06 delay 10 seconds @19:55:10.09 ARV.dial :air1 @19:55:10.49 adding ARV air @19:55:10.51 delay 2 seconds @19:55:12.61 AS.seek 0.536ml @19:55:19.13 delay 5 seconds @19:55:24.16 ARV.dial :carrier @19:55:24.59 delay 7 seconds @19:55:31.68 AS.seek 0.594ml @19:56:30.37 delay 5 seconds @19:56:35.40 ATV.dial :ASV @19:56:35.91 delay 2 seconds @19:56:38.58 AS.seek 0.6ml @19:56:44.87 delay 4 seconds @19:56:48.90 ATV.dial ARV @19:56:49.57 delay 2 seconds @19:56:51.60 ARV.dial :carrier @19:56:51.62 delay 2 seconds @19:56:53.72 AS.seek 0.65ml @19:57:44.20 delay 4 seconds @19:57:48.23 ATV.dial PCR @19:57:48.87 ASV.dial :waste1 @19:57:48.89 ARV.dial :air1 @19:57:49.33 Positioning Slug by 0.324ml @19:57:49.44 AS.seek 0.326ml @20:03:15.77 delay 5 seconds @20:03:20.80 ATV.dial ARV @20:03:21.60 ASV.dial :PCRlock @20:03:22.02 delay 5 seconds @20:03:27.05 Running NM Profile60C300 thermocycling @20:03:27.06 PCR.program Profile60C300 @20:03:27.19 Profile60C300 should complete in < 1:40:53 @20:03:27.21 PCR.run 2:11:06.25 @21:25:02.00 PCR.off @21:25:02.58 PCR for NM completed -- closed /var/log/esp/NM20may0119h.pcr @21:25:02.67 Cleaning PCR @21:25:02.68 AFV.select :carrierSolution @21:25:02.82 delay 2 seconds @21:25:04.85 AS.reconfigure ASconfig @21:25:04.92 AS.seek 0.5ml @21:25:15.23 delay 1 second @21:25:16.25 AFV.select :holdingCoil @21:25:16.36 ASV.dial :waste1 @21:25:16.78 ATV.dial :air3 @21:25:18.71 AS.reconfigure ASslow1 @21:25:18.78 AS.seek 0.51ml @21:25:21.10 delay 2 seconds @21:25:23.12 ATV.dial :bleach @21:25:23.75 AS.seek 0.81ml @21:26:24.35 delay 3 seconds @21:26:27.38 ATV.dial :air3 @21:26:27.95 delay 3 seconds @21:26:31.04 AS.seek 0.835ml @21:26:36.37 delay 3 seconds @21:26:39.40 ATV.dial :bleach @21:26:40.03 AS.seek 0.82ml @21:26:43.35 delay 3 seconds @21:26:46.38 ARV.dial :waste @21:26:47.01 delay 3 seconds @21:26:50.04 ATV.dial :ARV @21:26:50.96 AS.reconfigure ASconfig @21:26:51.03 AS.seek 0.47ml @21:27:11.42 AFV.select :carrierSolution @21:27:11.56 delay 2 seconds @21:27:13.60 AS.seek 1ml @21:27:44.35 delay 1 second @21:27:45.37 AFV.select :holdingCoil @21:27:45.49 delay 1 second @21:27:46.52 AS.reconfigure ASfast @21:27:46.59 AS.seek 0ml @21:28:13.95 AFV.select :carrierSolution @21:28:14.08 delay 2 seconds @21:28:16.11 AS.reconfigure ASconfig @21:28:16.18 AS.seek 1ml @21:29:13.96 delay 1 second @21:29:14.98 AFV.select :holdingCoil @21:29:15.10 delay 1 second @21:29:16.13 AS.reconfigure ASfast @21:29:16.20 AS.seek 0ml @21:29:43.54 AFV.select :carrierSolution @21:29:43.67 delay 2 seconds @21:29:45.70 AS.reconfigure ASconfig @21:29:45.77 AS.seek 1ml @21:30:43.54 delay 1 second @21:30:44.56 AFV.select :holdingCoil @21:30:44.68 delay 1 second @21:30:45.71 AS.reconfigure ASfast @21:30:45.79 AS.seek 0ml @21:31:13.17 ARV.dial between carrier and air1 @21:31:13.68 ATV.dial :air3 @21:31:15.00 AS.reconfigure ASslow1 @21:31:15.07 AS.seek 0.01ml @21:31:17.39 delay 2 seconds @21:31:19.41 ATV.dial :bleach @21:31:20.05 AS.seek 0.81ml @21:34:01.21 delay 3 seconds @21:34:04.24 ATV.dial :air3 @21:34:04.81 delay 3 seconds @21:34:07.90 AS.seek 0.835ml @21:34:13.29 delay 3 seconds @21:34:16.31 ATV.dial :bleach @21:34:16.94 AS.seek 0.82ml @21:34:20.25 delay 3 seconds @21:34:23.28 ATV.dial PCR @21:34:23.70 AS.reconfigure ASconfig @21:34:23.76 AS.seek :empty @21:35:11.23 PCR.program :bleachProfile @21:35:11.29 PCR program should complete in < 2:45 @21:35:11.31 PCR.run 10:00 @21:38:07.09 PCR.off @21:38:07.13 AFV.select :carrierSolution @21:38:07.26 delay 2 seconds @21:38:09.30 AS.seek 1ml @21:39:07.20 delay 1 second @21:39:08.22 AFV.select :holdingCoil @21:39:08.34 delay 1 second @21:39:09.37 AS.reconfigure ASfast @21:39:09.44 AS.seek 0ml @21:39:36.81 delay 3 seconds @21:39:39.84 AFV.select :carrierSolution @21:39:39.96 delay 2 seconds @21:39:41.99 AS.reconfigure ASconfig @21:39:42.07 AS.seek 1ml @21:40:39.84 delay 1 second @21:40:40.86 AFV.select :holdingCoil @21:40:40.98 delay 1 second @21:40:42.01 AS.reconfigure ASfast @21:40:42.08 AS.seek 0ml @21:41:09.46 delay 3 seconds @21:41:12.49 AFV.select :carrierSolution @21:41:12.61 delay 2 seconds @21:41:14.64 AS.reconfigure ASconfig @21:41:14.72 AS.seek 1ml @21:42:12.51 delay 1 second @21:42:13.53 AFV.select :holdingCoil @21:42:13.65 delay 1 second @21:42:15.18 AS.reconfigure ASfast @21:42:15.33 AS.seek 0ml @21:42:42.70 delay 3 seconds @21:42:45.73 AFV.select :carrierSolution @21:42:45.85 delay 2 seconds @21:42:47.88 AS.reconfigure ASconfig @21:42:47.95 AS.seek 1ml @21:43:45.73 delay 1 second @21:43:46.75 AFV.select :holdingCoil @21:43:46.87 delay 1 second @21:43:47.90 AS.reconfigure ASfast @21:43:47.97 AS.seek 0ml @21:44:15.31 delay 3 seconds @21:44:18.34 AFV.select :carrierSolution @21:44:18.46 delay 2 seconds @21:44:20.49 AS.reconfigure ASconfig @21:44:20.57 AS.seek 1ml @21:45:18.34 delay 1 second @21:45:19.36 AFV.select :holdingCoil @21:45:19.48 delay 1 second @21:45:20.51 AS.reconfigure ASnorm @21:45:20.58 AS.seek 0ml @21:46:11.34 Setting up PCR for IPc -- writing /var/log/esp/IPc20may0121h.pcr @21:46:11.36 Building PCR Slug from ASV with MM3 mix, PP6 primer @21:46:11.40 Priming with carrier @21:46:11.42 ATV.dial ARV @21:46:12.07 ARV.dial :carrier @21:46:12.44 AS.seek 0.5ml @21:46:37.97 AFV.select :carrierSolution @21:46:38.11 delay 2 seconds @21:46:40.14 AS.reconfigure ASconfig @21:46:40.22 AS.seek 1ml @21:47:09.29 delay 1 second @21:47:10.31 AFV.select :holdingCoil @21:47:10.43 delay 1 second @21:47:11.46 ATV.dial :waste @21:47:13.15 AS.reconfigure ASnorm @21:47:13.22 AS.seek :empty @21:48:04.00 AFV.select :carrierSolution @21:48:04.13 delay 2 seconds @21:48:06.16 AS.reconfigure ASconfig @21:48:06.23 AS.seek 0.5ml @21:48:35.35 delay 1 second @21:48:36.38 AFV.select :holdingCoil @21:48:36.50 Building Slug @21:48:36.52 ATV.dial :ARV @21:48:38.20 delay 2 seconds @21:48:40.23 ARV.dial :air1 @21:48:40.65 delay 2 seconds @21:48:42.73 AS.reconfigure ASslow2 @21:48:42.81 AS.seek 0.506ml @21:48:49.14 delay 2 seconds @21:48:51.16 ARV.dial :MM3 @21:48:52.23 delay 2 seconds @21:48:54.26 Adding MM3 @21:48:54.36 AS.seek 0.524ml @21:49:12.90 delay 25 seconds @21:49:37.93 ARV.dial :PP6 @21:49:38.34 Adding PP6 @21:49:38.36 delay 2 seconds @21:49:40.46 AS.seek 0.53ml @21:49:46.96 delay 10 seconds @21:49:56.99 ARV.dial :air1 @21:49:57.82 adding ARV air @21:49:57.84 delay 2 seconds @21:49:59.93 AS.seek 0.536ml @21:50:06.59 delay 5 seconds @21:50:11.62 ARV.dial :carrier @21:50:12.04 delay 7 seconds @21:50:19.13 AS.seek 0.594ml @21:51:17.73 delay 5 seconds @21:51:22.76 ATV.dial :ASV @21:51:23.28 delay 2 seconds @21:51:25.37 AS.seek 0.6ml @21:51:31.81 delay 4 seconds @21:51:35.84 ATV.dial ARV @21:51:36.50 delay 2 seconds @21:51:38.53 ARV.dial :carrier @21:51:38.55 delay 2 seconds @21:51:40.64 AS.seek 0.65ml @21:52:31.32 delay 4 seconds @21:52:35.35 ATV.dial PCR @21:52:35.98 ASV.dial :waste1 @21:52:36.00 ARV.dial :air1 @21:52:36.44 Positioning Slug by 0.324ml @21:52:36.55 AS.seek 0.326ml @21:58:02.61 delay 5 seconds @21:58:07.64 ATV.dial ARV @21:58:08.43 ASV.dial :PCRlock @21:58:08.86 delay 5 seconds @21:58:13.89 Running IPc ProfileIPc thermocycling @21:58:13.91 PCR.program ProfileIPc @21:58:14.03 ProfileIPc should complete in < 1:40:53 @21:58:14.05 PCR.run 2:11:06.25 @23:20:39.41 PCR.off @23:20:40.00 PCR for IPc completed -- closed /var/log/esp/IPc20may0121h.pcr @23:20:40.08 Cleaning PCR @23:20:40.10 AFV.select :carrierSolution @23:20:40.23 delay 2 seconds @23:20:42.26 AS.reconfigure ASconfig @23:20:42.33 AS.seek 0.5ml @23:20:52.65 delay 1 second @23:20:53.67 AFV.select :holdingCoil @23:20:53.79 ASV.dial :waste1 @23:20:54.20 ATV.dial :air3 @23:20:55.51 AS.reconfigure ASslow1 @23:20:55.59 AS.seek 0.51ml @23:20:57.88 delay 2 seconds @23:20:59.90 ATV.dial :bleach @23:21:00.54 AS.seek 0.81ml @23:22:01.14 delay 3 seconds @23:22:04.17 ATV.dial :air3 @23:22:04.75 delay 3 seconds @23:22:07.83 AS.seek 0.835ml @23:22:13.14 delay 3 seconds @23:22:16.17 ATV.dial :bleach @23:22:16.82 AS.seek 0.82ml @23:22:20.15 delay 3 seconds @23:22:23.17 ARV.dial :waste @23:22:23.80 delay 3 seconds @23:22:26.83 ATV.dial :ARV @23:22:27.84 AS.reconfigure ASconfig @23:22:27.91 AS.seek 0.47ml @23:22:48.33 AFV.select :carrierSolution @23:22:48.47 delay 2 seconds @23:22:50.51 AS.seek 1ml @23:23:21.87 delay 1 second @23:23:22.89 AFV.select :holdingCoil @23:23:23.01 delay 1 second @23:23:24.04 AS.reconfigure ASfast @23:23:24.11 AS.seek 0ml @23:23:51.47 AFV.select :carrierSolution @23:23:51.60 delay 2 seconds @23:23:53.63 AS.reconfigure ASconfig @23:23:53.70 AS.seek 1ml @23:24:51.50 delay 1 second @23:24:52.52 AFV.select :holdingCoil @23:24:52.64 delay 1 second @23:24:53.67 AS.reconfigure ASfast @23:24:53.74 AS.seek 0ml @23:25:21.12 AFV.select :carrierSolution @23:25:21.24 delay 2 seconds @23:25:23.27 AS.reconfigure ASconfig @23:25:23.34 AS.seek 1ml @23:26:21.12 delay 1 second @23:26:22.14 AFV.select :holdingCoil @23:26:22.26 delay 1 second @23:26:23.29 AS.reconfigure ASfast @23:26:23.36 AS.seek 0ml @23:26:50.73 ARV.dial between carrier and air1 @23:26:51.25 ATV.dial :air3 @23:26:52.57 AS.reconfigure ASslow1 @23:26:52.64 AS.seek 0.01ml @23:26:54.97 delay 2 seconds @23:26:56.99 ATV.dial :bleach @23:26:57.65 AS.seek 0.81ml @23:29:38.81 delay 3 seconds @23:29:41.84 ATV.dial :air3 @23:29:42.42 delay 3 seconds @23:29:45.51 AS.seek 0.835ml @23:29:51.59 delay 3 seconds @23:29:54.62 ATV.dial :bleach @23:29:55.27 AS.seek 0.82ml @23:29:58.58 delay 3 seconds @23:30:01.61 ATV.dial PCR @23:30:02.01 AS.reconfigure ASconfig @23:30:02.08 AS.seek :empty @23:30:49.56 PCR.program :bleachProfile @23:30:49.63 PCR program should complete in < 2:45 @23:30:49.64 PCR.run 10:00 @23:33:45.42 PCR.off @23:33:45.46 AFV.select :carrierSolution @23:33:45.59 delay 2 seconds @23:33:47.63 AS.seek 1ml @23:34:45.48 delay 1 second @23:34:46.50 AFV.select :holdingCoil @23:34:46.62 delay 1 second @23:34:47.65 AS.reconfigure ASfast @23:34:47.72 AS.seek 0ml @23:35:15.06 delay 3 seconds @23:35:18.09 AFV.select :carrierSolution @23:35:18.21 delay 2 seconds @23:35:20.24 AS.reconfigure ASconfig @23:35:20.31 AS.seek 1ml @23:36:18.11 delay 1 second @23:36:19.13 AFV.select :holdingCoil @23:36:19.25 delay 1 second @23:36:20.28 AS.reconfigure ASfast @23:36:20.35 AS.seek 0ml @23:36:47.69 delay 3 seconds @23:36:50.72 AFV.select :carrierSolution @23:36:50.84 delay 2 seconds @23:36:52.87 AS.reconfigure ASconfig @23:36:52.95 AS.seek 1ml @23:37:50.73 delay 1 second @23:37:51.75 AFV.select :holdingCoil @23:37:51.87 delay 1 second @23:37:52.90 AS.reconfigure ASfast @23:37:52.97 AS.seek 0ml @23:38:20.31 delay 3 seconds @23:38:23.34 AFV.select :carrierSolution @23:38:23.46 delay 2 seconds @23:38:25.49 AS.reconfigure ASconfig @23:38:25.57 AS.seek 1ml @23:39:23.34 delay 1 second @23:39:24.36 AFV.select :holdingCoil @23:39:24.48 delay 1 second @23:39:25.51 AS.reconfigure ASfast @23:39:25.58 AS.seek 0ml @23:39:52.95 delay 3 seconds @23:39:55.98 AFV.select :carrierSolution @23:39:56.10 delay 2 seconds @23:39:58.13 AS.reconfigure ASconfig @23:39:58.20 AS.seek 1ml @23:40:56.01 delay 1 second @23:40:57.03 AFV.select :holdingCoil @23:40:57.15 delay 1 second @23:40:58.18 AS.reconfigure ASnorm @23:40:58.25 AS.seek 0ml @23:41:48.99 Setting up PCR for IPc2 -- writing /var/log/esp/IPc220may0123h.pcr @23:41:49.01 Building PCR Slug from ASV with MM2 mix, PP3 primer @23:41:49.04 Priming with carrier @23:41:49.06 ATV.dial ARV @23:41:49.72 ARV.dial :carrier @23:41:50.10 AS.seek 0.5ml @23:42:15.75 AFV.select :carrierSolution @23:42:15.89 delay 2 seconds @23:42:18.50 AS.reconfigure ASconfig @23:42:18.58 AS.seek 1ml @23:42:47.59 delay 1 second @23:42:48.61 AFV.select :holdingCoil @23:42:48.73 delay 1 second @23:42:49.76 ATV.dial :waste @23:42:51.45 AS.reconfigure ASnorm @23:42:51.53 AS.seek :empty @23:43:42.30 AFV.select :carrierSolution @23:43:42.43 delay 2 seconds @23:43:44.46 AS.reconfigure ASconfig @23:43:44.53 AS.seek 0.5ml @23:44:13.66 delay 1 second @23:44:14.68 AFV.select :holdingCoil @23:44:14.80 Building Slug @23:44:14.81 ATV.dial :ARV @23:44:16.50 delay 2 seconds @23:44:18.53 ARV.dial :air1 @23:44:18.96 delay 2 seconds @23:44:21.05 AS.reconfigure ASslow2 @23:44:21.12 AS.seek 0.506ml @23:44:27.41 delay 2 seconds @23:44:29.43 ARV.dial :MM2 @23:44:30.94 delay 2 seconds @23:44:32.97 Adding MM2 @23:44:33.08 AS.seek 0.524ml @23:44:51.39 delay 25 seconds @23:45:16.42 ARV.dial :PP3 @23:45:16.84 Adding PP3 @23:45:16.86 delay 2 seconds @23:45:18.95 AS.seek 0.53ml @23:45:25.50 delay 10 seconds @23:45:35.53 ARV.dial :air2 @23:45:35.95 adding ARV air @23:45:35.97 delay 2 seconds @23:45:38.07 AS.seek 0.536ml @23:45:44.34 delay 5 seconds @23:45:49.37 ARV.dial :carrier @23:45:50.66 delay 7 seconds @23:45:57.75 AS.seek 0.594ml @23:46:56.23 delay 5 seconds @23:47:01.26 ATV.dial :ASV @23:47:01.69 delay 2 seconds @23:47:03.78 AS.seek 0.6ml @23:47:10.09 delay 4 seconds @23:47:14.12 ATV.dial ARV @23:47:14.69 delay 2 seconds @23:47:16.72 ARV.dial :carrier @23:47:16.74 delay 2 seconds @23:47:18.84 AS.seek 0.65ml @23:48:09.56 delay 4 seconds @23:48:13.59 ATV.dial PCR @23:48:14.22 ASV.dial :waste1 @23:48:14.24 ARV.dial :air1 @23:48:14.67 Positioning Slug by 0.324ml @23:48:14.78 AS.seek 0.326ml @23:53:40.62 delay 5 seconds @23:53:45.65 ATV.dial ARV @23:53:46.45 ASV.dial :PCRlock @23:53:46.87 delay 5 seconds @23:53:51.90 Running IPc2 ProfileIPc thermocycling @23:53:51.91 PCR.program ProfileIPc @23:53:52.03 ProfileIPc should complete in < 1:40:53 @23:53:52.05 PCR.run 2:11:06.25 @01:16:13.66CEST02-May-20 PCR.off @01:16:14.25 PCR for IPc2 completed -- closed /var/log/esp/IPc220may0123h.pcr @01:16:14.27 Full MFB Decontamination @01:16:14.30 Decon extraction column and decon AIV @01:16:14.33 SPE.hold 2:46:40,55C @01:16:14.40 ASV.dial :SPEdelivery @01:16:15.08 delay 20 seconds @01:16:35.11 AFV.select :carrierSolution @01:16:35.23 delay 2 seconds @01:16:37.26 AS.reconfigure ASconfig @01:16:37.33 AS.seek 0.5ml @01:16:47.66 delay 1 second @01:16:48.68 AFV.select :holdingCoil @01:16:48.80 ATV.dial :air1 @01:16:50.82 AS.reconfigure ASslow1 @01:16:50.89 AS.seek 0.52ml @01:16:55.20 ATV.dial :ASV @01:16:57.08 ASV.dial :SPErecovery @01:16:57.81 AS.seek 0.62ml @01:17:18.23 ATV.dial :waste @01:17:20.36 AS.reconfigure ASnorm @01:17:20.44 AS.seek :empty @01:17:52.02 AFV.select :holdingCoil @01:17:52.04 ATV.dial :waste @01:17:52.08 AS.reconfigure ASconfig @01:17:52.15 AS.seek 0ml @01:17:52.45 AFV.select :carrierSolution @01:17:52.58 delay 2 seconds @01:17:54.62 AS.seek 0.5ml @01:18:23.66 delay 1 second @01:18:24.68 AFV.select :holdingCoil @01:18:24.80 ATV.dial :air3 @01:18:25.57 AS.reconfigure ASslow1 @01:18:25.64 AS.seek 0.52ml @01:18:29.99 ATV.dial :air3 @01:18:30.09 AS.seek 0.53ml @01:18:32.39 delay 2 seconds @01:18:34.41 ATV.dial :bleach @01:18:34.90 AS.seek 0.71ml @01:19:11.39 delay 3 seconds @01:19:14.42 ATV.dial :air3 @01:19:15.03 delay 3 seconds @01:19:18.12 AS.seek 0.735ml @01:19:23.50 delay 3 seconds @01:19:26.53 ATV.dial :bleach @01:19:27.21 AS.seek 0.72ml @01:19:30.53 delay 3 seconds @01:19:33.56 ATV.dial ASV @01:19:34.69 ASV.dial :SPErecovery @01:19:34.79 AS.seek 0.365ml @01:20:46.45 ATV.dial :waste @01:20:48.28 AS.reconfigure ASnorm @01:20:48.94 AS.seek :empty @01:21:07.74 ATV.dial :air3 @01:21:08.46 AS.reconfigure ASslow1 @01:21:08.53 AS.seek 0.009ml @01:21:10.86 delay 2 seconds @01:21:12.88 ATV.dial :bleach @01:21:13.37 AS.seek 0.579ml @01:23:08.28 delay 3 seconds @01:23:11.31 ATV.dial :air3 @01:23:11.89 delay 3 seconds @01:23:14.98 AS.seek 0.604ml @01:23:20.33 delay 3 seconds @01:23:23.36 ATV.dial :bleach @01:23:24.01 AS.seek 0.589ml @01:23:27.35 delay 3 seconds @01:23:30.38 ATV.dial :ASV @01:23:31.44 ASV.dial :wasteR @01:23:31.87 AS.seek 0ml @01:25:30.63 delay 1:00 @01:26:30.66 ATV.dial ASV @01:26:30.67 AFV.select :carrierSolution @01:26:30.80 delay 2 seconds @01:26:32.83 AS.reconfigure ASconfig @01:26:32.90 AS.seek 1ml @01:27:30.67 delay 1 second @01:27:31.69 AFV.select :holdingCoil @01:27:31.81 delay 1 second @01:27:32.84 ASV.dial :wasteR @01:27:32.86 AS.reconfigure ASslow1 @01:27:32.94 AS.seek 0ml @01:30:54.35 delay 10 seconds @01:31:04.38 ATV.dial :air3 @01:31:05.93 AS.seek 0.01ml @01:31:08.24 delay 2 seconds @01:31:10.26 ATV.dial :bleach @01:31:10.92 AS.seek 0.58ml @01:33:05.85 delay 3 seconds @01:33:08.88 ATV.dial :air3 @01:33:09.47 delay 3 seconds @01:33:12.56 AS.seek 0.605ml @01:33:17.90 delay 3 seconds @01:33:20.93 ATV.dial :bleach @01:33:21.59 AS.seek 0.59ml @01:33:24.91 delay 3 seconds @01:33:27.94 ATV.dial ASV @01:33:29.00 ASV.dial :SPEdelivery @01:33:29.40 AS.seek 0ml @01:35:28.31 delay 1:00 @01:36:28.34 AFV.select :carrierSolution @01:36:28.46 delay 2 seconds @01:36:30.49 AS.reconfigure ASconfig @01:36:30.56 AS.seek 1ml @01:37:28.38 delay 1 second @01:37:29.40 AFV.select :holdingCoil @01:37:29.52 delay 1 second @01:37:30.55 ATV.dial ASV @01:37:30.56 ASV.dial :SPEdelivery @01:37:30.60 AS.reconfigure ASslow1 @01:37:30.68 AS.seek 0ml @01:40:52.07 delay 10 seconds @01:41:02.10 ATV.dial :air2 @01:41:02.96 AS.reconfigure ASslow0 @01:41:03.04 AS.seek 0.8ml @01:42:23.78 delay 3 seconds @01:42:26.81 ATV.dial :ASV @01:42:27.83 ASV.dial :SPEdelivery @01:42:27.85 AS.reconfigure ASslow1 @01:42:27.93 AS.seek 0.03ml @01:45:03.08 delay 20 seconds @01:45:23.11 ATV.dial :waste,via: :air3 @01:45:24.88 AS.reconfigure ASconfig @01:45:24.95 AS.seek :empty @01:45:27.06 SPE.coast @01:45:27.17 Removing Bleach from Mixing Coil @01:45:27.18 AFV.select :carrierSolution @01:45:27.31 delay 2 seconds @01:45:29.35 AS.seek 0.5ml @01:45:58.47 delay 1 second @01:45:59.49 AFV.select :holdingCoil @01:45:59.61 ATV.dial :ASV @01:46:01.33 ASV.dial :SPErecovery @01:46:02.00 AS.reconfigure ASslow1 @01:46:02.08 AS.seek 0.73ml @01:46:48.61 ASV.dial :wasteD @01:46:49.21 AS.reconfigure ASnorm @01:46:49.28 AS.seek 0ml @01:47:26.41 AFV.select :carrierSolution @01:47:26.55 delay 2 seconds @01:47:28.58 AS.reconfigure ASconfig @01:47:28.65 AS.seek 0.5ml @01:47:57.71 delay 1 second @01:47:58.73 AFV.select :holdingCoil @01:47:58.85 ATV.dial ASV @01:47:58.87 ASV.dial :SPErecovery @01:48:00.15 AS.reconfigure ASslow0 @01:48:00.22 AS.seek 0.3ml @01:48:20.67 ATV.dial :air2 @01:48:21.74 AS.reconfigure ASslow1 @01:48:21.81 AS.seek 0.32ml @01:48:26.16 ATV.dial ASV @01:48:27.24 AS.reconfigure ASslow0 @01:48:27.31 AS.seek 0.57ml @01:48:52.77 ASV.dial :wasteD @01:48:53.38 AS.reconfigure ASnorm @01:48:53.46 AS.seek 0ml @01:49:22.55 AFV.select :carrierSolution @01:49:22.68 delay 2 seconds @01:49:24.71 AS.reconfigure ASconfig @01:49:24.78 AS.seek 0.5ml @01:49:53.83 delay 1 second @01:49:54.85 AFV.select :holdingCoil @01:49:54.97 ATV.dial ASV @01:49:54.99 ASV.dial :SPErecovery @01:49:55.67 AS.reconfigure ASslow0 @01:49:55.74 AS.seek 0.3ml @01:50:16.16 ATV.dial :air2 @01:50:17.23 AS.reconfigure ASslow1 @01:50:17.30 AS.seek 0.32ml @01:50:21.67 ATV.dial ASV @01:50:22.74 AS.reconfigure ASslow0 @01:50:22.81 AS.seek 0.57ml @01:50:48.33 ASV.dial :wasteD @01:50:48.92 AS.reconfigure ASnorm @01:50:49.00 AS.seek 0ml @01:51:18.13 ATV.dial :air2 @01:51:19.14 AS.reconfigure ASslow0 @01:51:19.22 AS.seek 0.3ml @01:51:49.77 ATV.dial :ASV @01:51:50.78 ASV.dial :wasteD @01:51:50.80 AS.reconfigure ASconfig @01:51:50.88 AS.seek 0.05ml @01:52:05.59 ATV.dial :waste @01:52:07.50 AS.seek 0ml @01:52:10.69 ASV.dial :SPEdelivery @01:52:11.72 deconLysateINT @01:52:11.73 ATV.dial :air3 @01:52:12.45 AS.reconfigure ASslow1 @01:52:12.52 AS.seek 0.01ml @01:52:14.85 delay 2 seconds @01:52:16.87 ATV.dial :bleach @01:52:17.35 AS.seek 0.96ml @01:55:28.66 delay 3 seconds @01:55:31.69 ATV.dial :air3 @01:55:32.27 delay 3 seconds @01:55:35.36 AS.seek 0.985ml @01:55:40.67 delay 3 seconds @01:55:43.70 ATV.dial :bleach @01:55:44.45 AS.seek 0.97ml @01:55:47.78 delay 3 seconds @01:55:50.81 ATV.dial AIV @01:55:52.08 AIV.dial :coilToMFB @01:55:52.10 AWV.select :waste @01:55:52.12 AFV.select :carrierSolution @01:55:52.26 delay 2 seconds @01:55:54.29 AS.reconfigure ASconfig @01:55:54.36 AS.seek 1ml @01:55:56.42 delay 1 second @01:55:57.44 AFV.select :holdingCoil @01:55:57.56 delay 1 second @01:55:58.59 AS.reconfigure ASslow0 @01:55:58.66 AS.seek :empty @01:57:39.68 AFV.select :carrierSolution @01:57:39.82 delay 2 seconds @01:57:41.85 AS.reconfigure ASconfig @01:57:41.92 AS.seek 1ml @01:58:39.74 delay 1 second @01:58:40.76 AFV.select :holdingCoil @01:58:40.93 delay 1 second @01:58:41.96 AS.reconfigure ASslow0 @01:58:42.03 AS.seek :empty @02:00:22.99 AFV.select :carrierSolution @02:00:23.13 delay 2 seconds @02:00:25.16 AS.reconfigure ASconfig @02:00:25.23 AS.seek 1ml @02:01:23.08 delay 1 second @02:01:24.10 AFV.select :holdingCoil @02:01:24.22 delay 1 second @02:01:25.25 AS.reconfigure ASslow0 @02:01:25.32 AS.seek :empty @02:03:06.30 ATV.dial :air2 @02:03:07.00 AS.reconfigure ASslow1 @02:03:07.65 AS.seek 0.009ml @02:03:09.96 AS.reconfigure ASslow0 @02:03:10.04 AS.seek 1ml @02:04:49.99 ATV.dial AIV @02:04:50.79 AS.seek 0.02ml @02:06:29.64 ATV.dial :waste @02:06:31.42 AS.seek 0ml @02:06:33.76 AWV.select :air @02:06:33.95 Cleaning PCR @02:06:33.96 AFV.select :carrierSolution @02:06:34.10 delay 2 seconds @02:06:36.13 AS.reconfigure ASconfig @02:06:36.20 AS.seek 0.5ml @02:07:05.25 delay 1 second @02:07:06.27 AFV.select :holdingCoil @02:07:06.39 ASV.dial :waste1 @02:07:06.77 ATV.dial :air3 @02:07:07.48 AS.reconfigure ASslow1 @02:07:07.55 AS.seek 0.51ml @02:07:09.97 delay 2 seconds @02:07:11.99 ATV.dial :bleach @02:07:12.46 AS.seek 0.81ml @02:08:13.08 delay 3 seconds @02:08:16.11 ATV.dial :air3 @02:08:16.69 delay 3 seconds @02:08:19.78 AS.seek 0.835ml @02:08:25.11 delay 3 seconds @02:08:28.14 ATV.dial :bleach @02:08:28.78 AS.seek 0.82ml @02:08:32.11 delay 3 seconds @02:08:35.14 ARV.dial :waste @02:08:35.77 delay 3 seconds @02:08:38.80 ATV.dial :ARV @02:08:39.70 AS.reconfigure ASconfig @02:08:39.77 AS.seek 0.47ml @02:09:00.20 AFV.select :carrierSolution @02:09:00.34 delay 2 seconds @02:09:02.38 AS.seek 1ml @02:09:33.13 delay 1 second @02:09:34.15 AFV.select :holdingCoil @02:09:34.27 delay 1 second @02:09:35.30 AS.reconfigure ASfast @02:09:35.37 AS.seek 0ml @02:10:02.73 AFV.select :carrierSolution @02:10:02.86 delay 2 seconds @02:10:04.89 AS.reconfigure ASconfig @02:10:04.96 AS.seek 1ml @02:11:02.77 delay 1 second @02:11:03.79 AFV.select :holdingCoil @02:11:03.91 delay 1 second @02:11:04.94 AS.reconfigure ASfast @02:11:05.01 AS.seek 0ml @02:11:32.38 AFV.select :carrierSolution @02:11:32.51 delay 2 seconds @02:11:34.54 AS.reconfigure ASconfig @02:11:34.61 AS.seek 1ml @02:12:32.43 delay 1 second @02:12:33.45 AFV.select :holdingCoil @02:12:33.57 delay 1 second @02:12:34.60 AS.reconfigure ASfast @02:12:34.67 AS.seek 0ml @02:13:02.03 ARV.dial between carrier and air1 @02:13:02.57 ATV.dial :air3 @02:13:03.89 AS.reconfigure ASslow1 @02:13:03.96 AS.seek 0.01ml @02:13:06.43 delay 2 seconds @02:13:08.45 ATV.dial :bleach @02:13:09.10 AS.seek 0.81ml @02:15:50.33 delay 3 seconds @02:15:53.36 ATV.dial :air3 @02:15:53.94 delay 3 seconds @02:15:57.03 AS.seek 0.835ml @02:16:02.41 delay 3 seconds @02:16:05.44 ATV.dial :bleach @02:16:06.07 AS.seek 0.82ml @02:16:09.39 delay 3 seconds @02:16:12.42 ATV.dial PCR @02:16:12.83 AS.reconfigure ASconfig @02:16:12.90 AS.seek :empty @02:17:00.49 PCR.program :bleachProfile @02:17:00.55 PCR program should complete in < 2:45 @02:17:00.57 PCR.run 10:00 @02:20:02.98 PCR.off @02:20:03.02 AFV.select :carrierSolution @02:20:03.14 delay 2 seconds @02:20:05.18 AS.seek 1ml @02:21:03.02 delay 1 second @02:21:04.04 AFV.select :holdingCoil @02:21:04.16 delay 1 second @02:21:05.19 AS.reconfigure ASfast @02:21:05.26 AS.seek 0ml @02:21:32.63 delay 3 seconds @02:21:35.66 AFV.select :carrierSolution @02:21:35.78 delay 2 seconds @02:21:37.81 AS.reconfigure ASconfig @02:21:37.89 AS.seek 1ml @02:22:35.68 delay 1 second @02:22:36.70 AFV.select :holdingCoil @02:22:36.82 delay 1 second @02:22:37.85 AS.reconfigure ASfast @02:22:37.92 AS.seek 0ml @02:23:05.30 delay 3 seconds @02:23:08.33 AFV.select :carrierSolution @02:23:08.45 delay 2 seconds @02:23:10.48 AS.reconfigure ASconfig @02:23:10.56 AS.seek 1ml @02:24:08.32 delay 1 second @02:24:09.34 AFV.select :holdingCoil @02:24:09.46 delay 1 second @02:24:10.49 AS.reconfigure ASfast @02:24:10.56 AS.seek 0ml @02:24:37.91 delay 3 seconds @02:24:40.94 AFV.select :carrierSolution @02:24:41.06 delay 2 seconds @02:24:43.09 AS.reconfigure ASconfig @02:24:43.16 AS.seek 1ml @02:25:40.97 delay 1 second @02:25:41.99 AFV.select :holdingCoil @02:25:42.11 delay 1 second @02:25:43.14 AS.reconfigure ASfast @02:25:43.21 AS.seek 0ml @02:26:10.53 delay 3 seconds @02:26:13.56 AFV.select :carrierSolution @02:26:13.68 delay 2 seconds @02:26:15.71 AS.reconfigure ASconfig @02:26:15.79 AS.seek 1ml @02:27:13.57 delay 1 second @02:27:14.59 AFV.select :holdingCoil @02:27:14.71 delay 1 second @02:27:15.74 AS.reconfigure ASnorm @02:27:15.81 AS.seek 0ml @02:28:06.54 AFV.select :carrierSolution @02:28:06.67 delay 2 seconds @02:28:08.70 AS.reconfigure ASconfig @02:28:08.77 AS.seek 1ml @02:29:06.60 delay 1 second @02:29:07.62 AFV.select :holdingCoil @02:29:07.74 delay 1 second @02:29:08.77 ATV.dial :waste @02:29:10.00 AS.reconfigure ASnorm @02:29:10.08 AS.seek 0.5ml @02:29:35.64 ATV.dial :waste2 @02:29:36.94 AS.seek 0ml @02:30:02.50 Setting up PCR for OM -- writing /var/log/esp/OM20may0202h.pcr @02:30:02.52 Building PCR Slug from elutionWater with MM3 mix, PP5 primer @02:30:02.55 Priming with carrier @02:30:02.57 ATV.dial ARV @02:30:03.43 ARV.dial :carrier @02:30:03.81 AS.seek 0.5ml @02:30:29.37 AFV.select :carrierSolution @02:30:29.51 delay 2 seconds @02:30:31.54 AS.reconfigure ASconfig @02:30:31.61 AS.seek 1ml @02:31:00.71 delay 1 second @02:31:01.73 AFV.select :holdingCoil @02:31:01.85 delay 1 second @02:31:02.88 ATV.dial :waste @02:31:04.39 AS.reconfigure ASnorm @02:31:04.47 AS.seek :empty @02:31:55.24 AFV.select :carrierSolution @02:31:55.37 delay 2 seconds @02:31:57.40 AS.reconfigure ASconfig @02:31:57.47 AS.seek 0.5ml @02:32:26.60 delay 1 second @02:32:27.62 AFV.select :holdingCoil @02:32:27.74 Building Slug @02:32:27.76 ATV.dial :ARV @02:32:29.41 delay 2 seconds @02:32:31.44 ARV.dial :air1 @02:32:32.00 delay 2 seconds @02:32:34.08 AS.reconfigure ASslow2 @02:32:34.16 AS.seek 0.506ml @02:32:40.60 delay 2 seconds @02:32:42.63 ARV.dial :MM3 @02:32:43.69 delay 2 seconds @02:32:45.72 Adding MM3 @02:32:45.82 AS.seek 0.524ml @02:33:04.14 delay 25 seconds @02:33:29.17 ARV.dial :PP5 @02:33:29.58 Adding PP5 @02:33:29.60 delay 2 seconds @02:33:31.70 AS.seek 0.53ml @02:33:37.99 delay 10 seconds @02:33:48.02 ARV.dial :air3 @02:33:48.41 adding ARV air @02:33:48.43 delay 2 seconds @02:33:50.53 AS.seek 0.536ml @02:33:56.74 delay 5 seconds @02:34:01.77 ARV.dial :carrier @02:34:03.05 delay 7 seconds @02:34:10.14 AS.seek 0.594ml @02:35:09.44 delay 5 seconds @02:35:14.47 ATV.dial :elutionWater @02:35:15.93 delay 2 seconds @02:35:18.02 AS.seek 0.6ml @02:35:24.36 delay 4 seconds @02:35:28.39 ATV.dial ARV @02:35:29.83 delay 2 seconds @02:35:31.86 ARV.dial :carrier @02:35:31.88 delay 2 seconds @02:35:33.98 AS.seek 0.65ml @02:36:24.46 delay 4 seconds @02:36:28.49 ATV.dial PCR @02:36:29.29 ASV.dial :waste1 @02:36:29.31 ARV.dial :air1 @02:36:29.75 Positioning Slug by 0.324ml @02:36:29.86 AS.seek 0.326ml @02:41:56.10 delay 5 seconds @02:42:01.13 ATV.dial ARV @02:42:01.93 ASV.dial :PCRlock @02:42:02.33 delay 5 seconds @02:42:07.36 Running OM Profile60C350 thermocycling @02:42:07.37 PCR.program Profile60C350 @02:42:07.51 Profile60C350 should complete in < 1:46:29 @02:42:07.53 PCR.run 2:18:06.25 @04:04:58.38 PCR.off @04:05:00.01 PCR for OM completed -- closed /var/log/esp/OM20may0202h.pcr @04:05:00.10 Cleaning PCR @04:05:00.12 AFV.select :carrierSolution @04:05:00.25 delay 2 seconds @04:05:02.28 AS.reconfigure ASconfig @04:05:02.35 AS.seek 0.5ml @04:05:12.71 delay 1 second @04:05:13.73 AFV.select :holdingCoil @04:05:13.85 ASV.dial :waste1 @04:05:14.24 ATV.dial :air3 @04:05:15.56 AS.reconfigure ASslow1 @04:05:15.63 AS.seek 0.51ml @04:05:17.93 delay 2 seconds @04:05:19.96 ATV.dial :bleach @04:05:20.61 AS.seek 0.81ml @04:06:21.23 delay 3 seconds @04:06:24.26 ATV.dial :air3 @04:06:24.85 delay 3 seconds @04:06:27.94 AS.seek 0.835ml @04:06:33.26 delay 3 seconds @04:06:36.29 ATV.dial :bleach @04:06:36.95 AS.seek 0.82ml @04:06:40.26 delay 3 seconds @04:06:43.29 ARV.dial :waste @04:06:43.91 delay 3 seconds @04:06:46.94 ATV.dial :ARV @04:06:47.94 AS.reconfigure ASconfig @04:06:48.02 AS.seek 0.47ml @04:07:08.45 AFV.select :carrierSolution @04:07:08.59 delay 2 seconds @04:07:10.63 AS.seek 1ml @04:07:41.41 delay 1 second @04:07:42.43 AFV.select :holdingCoil @04:07:42.55 delay 1 second @04:07:43.58 AS.reconfigure ASfast @04:07:43.65 AS.seek 0ml @04:08:10.98 AFV.select :carrierSolution @04:08:11.11 delay 2 seconds @04:08:13.14 AS.reconfigure ASconfig @04:08:13.21 AS.seek 1ml @04:09:11.01 delay 1 second @04:09:12.03 AFV.select :holdingCoil @04:09:12.15 delay 1 second @04:09:13.18 AS.reconfigure ASfast @04:09:13.25 AS.seek 0ml @04:09:40.61 AFV.select :carrierSolution @04:09:40.75 delay 2 seconds @04:09:42.78 AS.reconfigure ASconfig @04:09:42.85 AS.seek 1ml @04:10:40.66 delay 1 second @04:10:41.68 AFV.select :holdingCoil @04:10:41.80 delay 1 second @04:10:42.83 AS.reconfigure ASfast @04:10:42.90 AS.seek 0ml @04:11:10.27 ARV.dial between carrier and air1 @04:11:10.79 ATV.dial :air3 @04:11:12.08 AS.reconfigure ASslow1 @04:11:12.16 AS.seek 0.01ml @04:11:14.49 delay 2 seconds @04:11:16.51 ATV.dial :bleach @04:11:17.15 AS.seek 0.81ml @04:13:58.33 delay 3 seconds @04:14:01.36 ATV.dial :air3 @04:14:01.93 delay 3 seconds @04:14:05.02 AS.seek 0.835ml @04:14:10.38 delay 3 seconds @04:14:13.40 ATV.dial :bleach @04:14:14.05 AS.seek 0.82ml @04:14:17.37 delay 3 seconds @04:14:20.94 ATV.dial PCR @04:14:21.37 AS.reconfigure ASconfig @04:14:21.44 AS.seek :empty @04:15:08.93 PCR.program :bleachProfile @04:15:08.99 PCR program should complete in < 2:45 @04:15:09.01 PCR.run 10:00 @04:18:04.80 PCR.off @04:18:04.84 AFV.select :carrierSolution @04:18:04.97 delay 2 seconds @04:18:07.01 AS.seek 1ml @04:19:04.87 delay 1 second @04:19:05.89 AFV.select :holdingCoil @04:19:06.01 delay 1 second @04:19:07.04 AS.reconfigure ASfast @04:19:07.11 AS.seek 0ml @04:19:34.46 delay 3 seconds @04:19:37.49 AFV.select :carrierSolution @04:19:37.61 delay 2 seconds @04:19:39.64 AS.reconfigure ASconfig @04:19:39.72 AS.seek 1ml @04:20:37.54 delay 1 second @04:20:38.56 AFV.select :holdingCoil @04:20:38.68 delay 1 second @04:20:39.71 AS.reconfigure ASfast @04:20:39.78 AS.seek 0ml @04:21:07.15 delay 3 seconds @04:21:10.18 AFV.select :carrierSolution @04:21:10.30 delay 2 seconds @04:21:12.33 AS.reconfigure ASconfig @04:21:12.41 AS.seek 1ml @04:22:10.18 delay 1 second @04:22:11.20 AFV.select :holdingCoil @04:22:11.32 delay 1 second @04:22:12.35 AS.reconfigure ASfast @04:22:12.42 AS.seek 0ml @04:22:39.77 delay 3 seconds @04:22:42.80 AFV.select :carrierSolution @04:22:42.92 delay 2 seconds @04:22:44.95 AS.reconfigure ASconfig @04:22:45.03 AS.seek 1ml @04:23:42.80 delay 1 second @04:23:43.82 AFV.select :holdingCoil @04:23:43.94 delay 1 second @04:23:44.97 AS.reconfigure ASfast @04:23:45.04 AS.seek 0ml @04:24:12.41 delay 3 seconds @04:24:15.44 AFV.select :carrierSolution @04:24:15.56 delay 2 seconds @04:24:17.59 AS.reconfigure ASconfig @04:24:17.67 AS.seek 1ml @04:25:15.44 delay 1 second @04:25:16.46 AFV.select :holdingCoil @04:25:16.63 delay 1 second @04:25:17.66 AS.reconfigure ASnorm @04:25:17.73 AS.seek 0ml @04:26:08.48 Setting up PCR for ML -- writing /var/log/esp/ML20may0204h.pcr @04:26:08.50 Building PCR Slug from elutionWater with MM2 mix, PP4 primer @04:26:08.53 Priming with carrier @04:26:08.55 ATV.dial ARV @04:26:09.21 ARV.dial :carrier @04:26:09.58 AS.seek 0.5ml @04:26:35.15 AFV.select :carrierSolution @04:26:35.29 delay 2 seconds @04:26:37.32 AS.reconfigure ASconfig @04:26:37.39 AS.seek 1ml @04:27:06.43 delay 1 second @04:27:07.45 AFV.select :holdingCoil @04:27:07.57 delay 1 second @04:27:08.60 ATV.dial :waste @04:27:10.30 AS.reconfigure ASnorm @04:27:10.37 AS.seek :empty @04:28:01.10 AFV.select :carrierSolution @04:28:01.23 delay 2 seconds @04:28:03.26 AS.reconfigure ASconfig @04:28:03.33 AS.seek 0.5ml @04:28:32.46 delay 1 second @04:28:33.48 AFV.select :holdingCoil @04:28:33.60 Building Slug @04:28:33.61 ATV.dial :ARV @04:28:35.38 delay 2 seconds @04:28:37.41 ARV.dial :air1 @04:28:37.82 delay 2 seconds @04:28:39.90 AS.reconfigure ASslow2 @04:28:39.98 AS.seek 0.506ml @04:28:46.35 delay 2 seconds @04:28:48.38 ARV.dial :MM2 @04:28:49.88 delay 2 seconds @04:28:51.91 Adding MM2 @04:28:52.02 AS.seek 0.524ml @04:29:10.59 delay 25 seconds @04:29:35.62 ARV.dial :PP4 @04:29:36.04 Adding PP4 @04:29:36.06 delay 2 seconds @04:29:38.16 AS.seek 0.53ml @04:29:44.57 delay 10 seconds @04:29:54.60 ARV.dial :air3 @04:29:55.01 adding ARV air @04:29:55.02 delay 2 seconds @04:29:57.12 AS.seek 0.536ml @04:30:03.32 delay 5 seconds @04:30:08.92 ARV.dial :carrier @04:30:10.21 delay 7 seconds @04:30:17.30 AS.seek 0.594ml @04:31:15.84 delay 5 seconds @04:31:20.87 ATV.dial :elutionWater @04:31:22.32 delay 2 seconds @04:31:24.41 AS.seek 0.6ml @04:31:30.66 delay 4 seconds @04:31:34.69 ATV.dial ARV @04:31:36.23 delay 2 seconds @04:31:38.26 ARV.dial :carrier @04:31:38.28 delay 2 seconds @04:31:40.38 AS.seek 0.65ml @04:32:30.88 delay 4 seconds @04:32:34.91 ATV.dial PCR @04:32:35.79 ASV.dial :waste1 @04:32:35.81 ARV.dial :air1 @04:32:36.24 Positioning Slug by 0.324ml @04:32:36.35 AS.seek 0.326ml @04:38:02.74 delay 5 seconds @04:38:07.77 ATV.dial ARV @04:38:08.55 ASV.dial :PCRlock @04:38:08.96 delay 5 seconds @04:38:13.99 Running ML Profile60C350 thermocycling @04:38:14.00 PCR.program Profile60C350 @04:38:14.15 Profile60C350 should complete in < 1:46:29 @04:38:14.17 PCR.run 2:18:06.25 @06:01:05.23 PCR.off @06:01:06.86 PCR for ML completed -- closed /var/log/esp/ML20may0204h.pcr @06:01:06.95 Cleaning PCR @06:01:06.96 AFV.select :carrierSolution @06:01:07.10 delay 2 seconds @06:01:09.13 AS.reconfigure ASconfig @06:01:09.20 AS.seek 0.5ml @06:01:19.56 delay 1 second @06:01:20.58 AFV.select :holdingCoil @06:01:20.70 ASV.dial :waste1 @06:01:21.10 ATV.dial :air3 @06:01:22.41 AS.reconfigure ASslow1 @06:01:22.48 AS.seek 0.51ml @06:01:24.79 delay 2 seconds @06:01:26.81 ATV.dial :bleach @06:01:27.47 AS.seek 0.81ml @06:02:28.07 delay 3 seconds @06:02:31.10 ATV.dial :air3 @06:02:31.68 delay 3 seconds @06:02:34.77 AS.seek 0.835ml @06:02:40.10 delay 3 seconds @06:02:43.13 ATV.dial :bleach @06:02:43.77 AS.seek 0.82ml @06:02:47.04 delay 3 seconds @06:02:50.07 ARV.dial :waste @06:02:50.70 delay 3 seconds @06:02:53.73 ATV.dial :ARV @06:02:54.73 AS.reconfigure ASconfig @06:02:54.81 AS.seek 0.47ml @06:03:15.23 AFV.select :carrierSolution @06:03:15.36 delay 2 seconds @06:03:17.40 AS.seek 1ml @06:03:48.15 delay 1 second @06:03:49.17 AFV.select :holdingCoil @06:03:49.29 delay 1 second @06:03:50.32 AS.reconfigure ASfast @06:03:50.39 AS.seek 0ml @06:04:17.75 AFV.select :carrierSolution @06:04:17.89 delay 2 seconds @06:04:19.92 AS.reconfigure ASconfig @06:04:19.99 AS.seek 1ml @06:05:17.79 delay 1 second @06:05:18.81 AFV.select :holdingCoil @06:05:18.93 delay 1 second @06:05:19.96 AS.reconfigure ASfast @06:05:20.03 AS.seek 0ml @06:05:47.38 AFV.select :carrierSolution @06:05:47.52 delay 2 seconds @06:05:49.55 AS.reconfigure ASconfig @06:05:49.62 AS.seek 1ml @06:06:47.43 delay 1 second @06:06:48.45 AFV.select :holdingCoil @06:06:48.57 delay 1 second @06:06:49.60 AS.reconfigure ASfast @06:06:49.67 AS.seek 0ml @06:07:17.03 ARV.dial between carrier and air1 @06:07:17.54 ATV.dial :air3 @06:07:18.85 AS.reconfigure ASslow1 @06:07:18.93 AS.seek 0.01ml @06:07:21.24 delay 2 seconds @06:07:23.26 ATV.dial :bleach @06:07:23.91 AS.seek 0.81ml @06:10:05.07 delay 3 seconds @06:10:08.10 ATV.dial :air3 @06:10:08.68 delay 3 seconds @06:10:11.77 AS.seek 0.835ml @06:10:17.08 delay 3 seconds @06:10:20.11 ATV.dial :bleach @06:10:20.77 AS.seek 0.82ml @06:10:24.12 delay 3 seconds @06:10:27.15 ATV.dial PCR @06:10:27.56 AS.reconfigure ASconfig @06:10:27.62 AS.seek :empty @06:11:15.10 PCR.program :bleachProfile @06:11:15.16 PCR program should complete in < 2:45 @06:11:15.17 PCR.run 10:00 @06:14:11.53 PCR.off @06:14:11.57 AFV.select :carrierSolution @06:14:11.69 delay 2 seconds @06:14:13.73 AS.seek 1ml @06:15:11.53 delay 1 second @06:15:12.55 AFV.select :holdingCoil @06:15:12.67 delay 1 second @06:15:13.70 AS.reconfigure ASfast @06:15:13.77 AS.seek 0ml @06:15:41.15 delay 3 seconds @06:15:44.18 AFV.select :carrierSolution @06:15:44.30 delay 2 seconds @06:15:46.33 AS.reconfigure ASconfig @06:15:46.40 AS.seek 1ml @06:16:44.21 delay 1 second @06:16:45.23 AFV.select :holdingCoil @06:16:45.35 delay 1 second @06:16:46.38 AS.reconfigure ASfast @06:16:46.45 AS.seek 0ml @06:17:13.79 delay 3 seconds @06:17:16.82 AFV.select :carrierSolution @06:17:16.94 delay 2 seconds @06:17:18.97 AS.reconfigure ASconfig @06:17:19.06 AS.seek 1ml @06:18:16.84 delay 1 second @06:18:17.86 AFV.select :holdingCoil @06:18:17.98 delay 1 second @06:18:19.01 AS.reconfigure ASfast @06:18:19.08 AS.seek 0ml @06:18:46.43 delay 3 seconds @06:18:49.46 AFV.select :carrierSolution @06:18:49.58 delay 2 seconds @06:18:51.61 AS.reconfigure ASconfig @06:18:51.75 AS.seek 1ml @06:19:49.53 delay 1 second @06:19:50.55 AFV.select :holdingCoil @06:19:50.67 delay 1 second @06:19:51.70 AS.reconfigure ASfast @06:19:51.77 AS.seek 0ml @06:20:19.15 delay 3 seconds @06:20:22.18 AFV.select :carrierSolution @06:20:22.30 delay 2 seconds @06:20:24.33 AS.reconfigure ASconfig @06:20:24.41 AS.seek 1ml @06:21:22.17 delay 1 second @06:21:23.19 AFV.select :holdingCoil @06:21:23.31 delay 1 second @06:21:24.34 AS.reconfigure ASnorm @06:21:24.41 AS.seek 0ml @06:22:15.14 Setting up PCR for CG -- writing /var/log/esp/CG20may0206h.pcr @06:22:15.16 Building PCR Slug from elutionWater with MM1 mix, PP2 primer @06:22:15.19 Priming with carrier @06:22:15.21 ATV.dial ARV @06:22:15.85 ARV.dial :carrier @06:22:16.22 AS.seek 0.5ml @06:22:41.75 AFV.select :carrierSolution @06:22:41.89 delay 2 seconds @06:22:43.92 AS.reconfigure ASconfig @06:22:44.00 AS.seek 1ml @06:23:13.03 delay 1 second @06:23:14.05 AFV.select :holdingCoil @06:23:14.17 delay 1 second @06:23:15.20 ATV.dial :waste @06:23:16.89 AS.reconfigure ASnorm @06:23:16.97 AS.seek :empty @06:24:07.70 AFV.select :carrierSolution @06:24:07.84 delay 2 seconds @06:24:09.87 AS.reconfigure ASconfig @06:24:09.94 AS.seek 0.5ml @06:24:39.07 delay 1 second @06:24:40.09 AFV.select :holdingCoil @06:24:40.21 Building Slug @06:24:40.23 ATV.dial :ARV @06:24:41.90 delay 2 seconds @06:24:43.93 ARV.dial :air1 @06:24:44.34 delay 2 seconds @06:24:46.42 AS.reconfigure ASslow2 @06:24:46.50 AS.seek 0.506ml @06:24:52.90 delay 2 seconds @06:24:54.92 ARV.dial :MM1 @06:24:55.54 delay 2 seconds @06:24:57.57 Adding MM1 @06:24:57.66 AS.seek 0.524ml @06:25:16.07 delay 25 seconds @06:25:41.10 ARV.dial :PP2 @06:25:41.51 Adding PP2 @06:25:41.53 delay 2 seconds @06:25:43.63 AS.seek 0.53ml @06:25:50.26 delay 10 seconds @06:26:00.29 ARV.dial :air2 @06:26:00.68 adding ARV air @06:26:00.70 delay 2 seconds @06:26:02.79 AS.seek 0.536ml @06:26:09.12 delay 5 seconds @06:26:14.15 ARV.dial :carrier @06:26:15.45 delay 7 seconds @06:26:22.54 AS.seek 0.594ml @06:27:21.04 delay 5 seconds @06:27:26.07 ATV.dial :elutionWater @06:27:28.10 delay 2 seconds @06:27:30.21 AS.seek 0.6ml @06:27:36.48 delay 4 seconds @06:27:40.51 ATV.dial ARV @06:27:41.97 delay 2 seconds @06:27:44.00 ARV.dial :carrier @06:27:44.02 delay 2 seconds @06:27:46.12 AS.seek 0.65ml @06:28:36.56 delay 4 seconds @06:28:40.59 ATV.dial PCR @06:28:41.37 ASV.dial :waste1 @06:28:41.39 ARV.dial :air1 @06:28:41.83 Positioning Slug by 0.324ml @06:28:41.94 AS.seek 0.326ml @06:34:07.82 delay 5 seconds @06:34:12.85 ATV.dial ARV @06:34:13.65 ASV.dial :PCRlock @06:34:14.06 delay 5 seconds @06:34:19.09 Running CG Profile60C300 thermocycling @06:34:19.10 PCR.program Profile60C300 @06:34:19.23 Profile60C300 should complete in < 1:40:53 @06:34:19.25 PCR.run 2:11:06.25 @07:55:48.22 PCR.off @07:55:48.80 PCR for CG completed -- closed /var/log/esp/CG20may0206h.pcr @07:55:48.90 Cleaning PCR @07:55:48.91 AFV.select :carrierSolution @07:55:49.04 delay 2 seconds @07:55:51.07 AS.reconfigure ASconfig @07:55:51.14 AS.seek 0.5ml @07:56:01.47 delay 1 second @07:56:02.49 AFV.select :holdingCoil @07:56:02.61 ASV.dial :waste1 @07:56:03.00 ATV.dial :air3 @07:56:04.92 AS.reconfigure ASslow1 @07:56:05.00 AS.seek 0.51ml @07:56:07.31 delay 2 seconds @07:56:09.33 ATV.dial :bleach @07:56:09.99 AS.seek 0.81ml @07:57:10.61 delay 3 seconds @07:57:13.64 ATV.dial :air3 @07:57:14.22 delay 3 seconds @07:57:17.31 AS.seek 0.835ml @07:57:22.63 delay 3 seconds @07:57:25.66 ATV.dial :bleach @07:57:26.30 AS.seek 0.82ml @07:57:29.62 delay 3 seconds @07:57:32.65 ARV.dial :waste @07:57:33.28 delay 3 seconds @07:57:36.31 ATV.dial :ARV @07:57:37.21 AS.reconfigure ASconfig @07:57:37.29 AS.seek 0.47ml @07:57:57.69 AFV.select :carrierSolution @07:57:57.83 delay 2 seconds @07:57:59.87 AS.seek 1ml @07:58:30.64 delay 1 second @07:58:31.66 AFV.select :holdingCoil @07:58:31.78 delay 1 second @07:58:32.81 AS.reconfigure ASfast @07:58:32.88 AS.seek 0ml @07:59:00.24 AFV.select :carrierSolution @07:59:00.37 delay 2 seconds @07:59:02.40 AS.reconfigure ASconfig @07:59:02.47 AS.seek 1ml @08:00:00.23 delay 1 second @08:00:01.25 AFV.select :holdingCoil @08:00:01.37 delay 1 second @08:00:02.40 AS.reconfigure ASfast @08:00:02.47 AS.seek 0ml @08:00:29.82 AFV.select :carrierSolution @08:00:29.96 delay 2 seconds @08:00:31.99 AS.reconfigure ASconfig @08:00:32.06 AS.seek 1ml @08:01:29.87 delay 1 second @08:01:30.89 AFV.select :holdingCoil @08:01:31.01 delay 1 second @08:01:32.04 AS.reconfigure ASfast @08:01:32.11 AS.seek 0ml @08:01:59.47 ARV.dial between carrier and air1 @08:01:59.98 ATV.dial :air3 @08:02:01.32 AS.reconfigure ASslow1 @08:02:01.40 AS.seek 0.01ml @08:02:03.78 delay 2 seconds @08:02:05.80 ATV.dial :bleach @08:02:06.46 AS.seek 0.81ml @08:04:47.59 delay 3 seconds @08:04:50.62 ATV.dial :air3 @08:04:51.20 delay 3 seconds @08:04:54.29 AS.seek 0.835ml @08:04:59.65 delay 3 seconds @08:05:02.68 ATV.dial :bleach @08:05:03.33 AS.seek 0.82ml @08:05:06.65 delay 3 seconds @08:05:09.67 ATV.dial PCR @08:05:10.11 AS.reconfigure ASconfig @08:05:10.19 AS.seek :empty @08:05:57.67 PCR.program :bleachProfile @08:05:57.73 PCR program should complete in < 2:45 @08:05:57.75 PCR.run 10:00 @08:08:53.53 PCR.off @08:08:53.57 AFV.select :carrierSolution @08:08:53.69 delay 2 seconds @08:08:55.72 AS.seek 1ml @08:09:53.58 delay 1 second @08:09:54.60 AFV.select :holdingCoil @08:09:54.72 delay 1 second @08:09:55.75 AS.reconfigure ASfast @08:09:55.82 AS.seek 0ml @08:10:23.19 delay 3 seconds @08:10:26.22 AFV.select :carrierSolution @08:10:26.34 delay 2 seconds @08:10:28.37 AS.reconfigure ASconfig @08:10:28.45 AS.seek 1ml @08:11:26.23 delay 1 second @08:11:27.25 AFV.select :holdingCoil @08:11:27.37 delay 1 second @08:11:28.40 AS.reconfigure ASfast @08:11:28.47 AS.seek 0ml @08:11:55.84 delay 3 seconds @08:11:58.87 AFV.select :carrierSolution @08:11:58.99 delay 2 seconds @08:12:01.02 AS.reconfigure ASconfig @08:12:01.10 AS.seek 1ml @08:12:58.94 delay 1 second @08:12:59.96 AFV.select :holdingCoil @08:13:00.67 delay 1 second @08:13:01.70 AS.reconfigure ASfast @08:13:01.77 AS.seek 0ml @08:13:29.15 delay 3 seconds @08:13:32.18 AFV.select :carrierSolution @08:13:32.30 delay 2 seconds @08:13:34.33 AS.reconfigure ASconfig @08:13:34.42 AS.seek 1ml @08:14:32.23 delay 1 second @08:14:33.25 AFV.select :holdingCoil @08:14:33.37 delay 1 second @08:14:34.40 AS.reconfigure ASfast @08:14:34.47 AS.seek 0ml @08:15:01.83 delay 3 seconds @08:15:04.86 AFV.select :carrierSolution @08:15:04.98 delay 2 seconds @08:15:07.01 AS.reconfigure ASconfig @08:15:07.09 AS.seek 1ml @08:16:04.89 delay 1 second @08:16:05.91 AFV.select :holdingCoil @08:16:06.03 delay 1 second @08:16:07.06 AS.reconfigure ASnorm @08:16:07.13 AS.seek 0ml @08:16:57.92 Setting up PCR for NM -- writing /var/log/esp/NM20may0208h.pcr @08:16:57.94 Building PCR Slug from elutionWater with MM1 mix, PP1 primer @08:16:57.97 Priming with carrier @08:16:57.99 ATV.dial ARV @08:16:58.64 ARV.dial :carrier @08:16:59.01 AS.seek 0.5ml @08:17:24.58 AFV.select :carrierSolution @08:17:24.72 delay 2 seconds @08:17:26.75 AS.reconfigure ASconfig @08:17:26.82 AS.seek 1ml @08:17:55.87 delay 1 second @08:17:56.89 AFV.select :holdingCoil @08:17:57.01 delay 1 second @08:17:58.04 ATV.dial :waste @08:17:59.73 AS.reconfigure ASnorm @08:17:59.81 AS.seek :empty @08:18:50.57 AFV.select :carrierSolution @08:18:50.71 delay 2 seconds @08:18:52.74 AS.reconfigure ASconfig @08:18:52.81 AS.seek 0.5ml @08:19:21.95 delay 1 second @08:19:22.97 AFV.select :holdingCoil @08:19:23.09 Building Slug @08:19:23.11 ATV.dial :ARV @08:19:24.78 delay 2 seconds @08:19:26.81 ARV.dial :air1 @08:19:27.20 delay 2 seconds @08:19:29.28 AS.reconfigure ASslow2 @08:19:29.36 AS.seek 0.506ml @08:19:35.55 delay 2 seconds @08:19:37.57 ARV.dial :MM1 @08:19:38.19 delay 2 seconds @08:19:40.22 Adding MM1 @08:19:40.32 AS.seek 0.524ml @08:19:58.58 delay 25 seconds @08:20:23.61 ARV.dial :PP1 @08:20:24.03 Adding PP1 @08:20:24.05 delay 2 seconds @08:20:26.15 AS.seek 0.53ml @08:20:32.56 delay 10 seconds @08:20:42.59 ARV.dial :air1 @08:20:42.98 adding ARV air @08:20:43.00 delay 2 seconds @08:20:45.10 AS.seek 0.536ml @08:20:51.64 delay 5 seconds @08:20:56.67 ARV.dial :carrier @08:20:57.09 delay 7 seconds @08:21:04.18 AS.seek 0.594ml @08:22:02.83 delay 5 seconds @08:22:07.86 ATV.dial :elutionWater @08:22:09.33 delay 2 seconds @08:22:11.42 AS.seek 0.6ml @08:22:17.59 delay 4 seconds @08:22:21.62 ATV.dial ARV @08:22:23.07 delay 2 seconds @08:22:25.10 ARV.dial :carrier @08:22:25.12 delay 2 seconds @08:22:27.21 AS.seek 0.65ml @08:23:17.73 delay 4 seconds @08:23:21.76 ATV.dial PCR @08:23:22.66 ASV.dial :waste1 @08:23:22.68 ARV.dial :air1 @08:23:23.11 Positioning Slug by 0.324ml @08:23:23.22 AS.seek 0.326ml @08:28:49.83 delay 5 seconds @08:28:54.86 ATV.dial ARV @08:28:55.64 ASV.dial :PCRlock @08:28:56.05 delay 5 seconds @08:29:01.08 Running NM Profile60C300 thermocycling @08:29:01.10 PCR.program Profile60C300 @08:29:01.23 Profile60C300 should complete in < 1:40:53 @08:29:01.25 PCR.run 2:11:06.25 @09:50:39.00 PCR.off @09:50:39.60 PCR for NM completed -- closed /var/log/esp/NM20may0208h.pcr @09:50:39.69 Cleaning PCR @09:50:39.70 AFV.select :carrierSolution @09:50:39.83 delay 2 seconds @09:50:41.86 AS.reconfigure ASconfig @09:50:41.93 AS.seek 0.5ml @09:50:52.27 delay 1 second @09:50:53.29 AFV.select :holdingCoil @09:50:53.41 ASV.dial :waste1 @09:50:53.81 ATV.dial :air3 @09:50:55.12 AS.reconfigure ASslow1 @09:50:55.19 AS.seek 0.51ml @09:50:57.50 delay 2 seconds @09:50:59.53 ATV.dial :bleach @09:51:00.18 AS.seek 0.81ml @09:52:00.80 delay 3 seconds @09:52:03.83 ATV.dial :air3 @09:52:04.41 delay 3 seconds @09:52:07.50 AS.seek 0.835ml @09:52:12.82 delay 3 seconds @09:52:15.85 ATV.dial :bleach @09:52:16.51 AS.seek 0.82ml @09:52:19.86 delay 3 seconds @09:52:22.88 ARV.dial :waste @09:52:23.52 delay 3 seconds @09:52:26.55 ATV.dial :ARV @09:52:27.45 AS.reconfigure ASconfig @09:52:27.52 AS.seek 0.47ml @09:52:47.94 AFV.select :carrierSolution @09:52:48.07 delay 2 seconds @09:52:50.11 AS.seek 1ml @09:53:20.86 delay 1 second @09:53:21.88 AFV.select :holdingCoil @09:53:22.00 delay 1 second @09:53:23.03 AS.reconfigure ASfast @09:53:23.70 AS.seek 0ml @09:53:51.07 AFV.select :carrierSolution @09:53:51.20 delay 2 seconds @09:53:53.23 AS.reconfigure ASconfig @09:53:53.30 AS.seek 1ml @09:54:51.09 delay 1 second @09:54:52.11 AFV.select :holdingCoil @09:54:52.23 delay 1 second @09:54:53.26 AS.reconfigure ASfast @09:54:53.33 AS.seek 0ml @09:55:20.68 AFV.select :carrierSolution @09:55:20.82 delay 2 seconds @09:55:22.85 AS.reconfigure ASconfig @09:55:22.92 AS.seek 1ml @09:56:20.74 delay 1 second @09:56:21.77 AFV.select :holdingCoil @09:56:21.89 delay 1 second @09:56:22.92 AS.reconfigure ASfast @09:56:22.99 AS.seek 0ml @09:56:50.35 ARV.dial between carrier and air1 @09:56:50.86 ATV.dial :air3 @09:56:52.18 AS.reconfigure ASslow1 @09:56:52.25 AS.seek 0.01ml @09:56:54.56 delay 2 seconds @09:56:56.58 ATV.dial :bleach @09:56:57.21 AS.seek 0.81ml @09:59:38.35 delay 3 seconds @09:59:41.38 ATV.dial :air3 @09:59:41.95 delay 3 seconds @09:59:45.04 AS.seek 0.835ml @09:59:50.35 delay 3 seconds @09:59:53.38 ATV.dial :bleach @09:59:54.02 AS.seek 0.82ml @09:59:57.35 delay 3 seconds @10:00:00.38 ATV.dial PCR @10:00:00.80 AS.reconfigure ASconfig @10:00:00.87 AS.seek :empty @10:00:48.38 PCR.program :bleachProfile @10:00:48.44 PCR program should complete in < 2:45 @10:00:48.46 PCR.run 10:00 @10:03:44.23 PCR.off @10:03:44.27 AFV.select :carrierSolution @10:03:44.39 delay 2 seconds @10:03:46.43 AS.seek 1ml @10:04:44.28 delay 1 second @10:04:45.30 AFV.select :holdingCoil @10:04:45.42 delay 1 second @10:04:46.45 AS.reconfigure ASfast @10:04:46.52 AS.seek 0ml @10:05:13.89 delay 3 seconds @10:05:16.92 AFV.select :carrierSolution @10:05:17.04 delay 2 seconds @10:05:19.07 AS.reconfigure ASconfig @10:05:19.15 AS.seek 1ml @10:06:16.95 delay 1 second @10:06:17.97 AFV.select :holdingCoil @10:06:18.09 delay 1 second @10:06:19.12 AS.reconfigure ASfast @10:06:19.19 AS.seek 0ml @10:06:46.55 delay 3 seconds @10:06:49.58 AFV.select :carrierSolution @10:06:49.70 delay 2 seconds @10:06:51.73 AS.reconfigure ASconfig @10:06:51.81 AS.seek 1ml @10:07:49.67 delay 1 second @10:07:50.69 AFV.select :holdingCoil @10:07:50.81 delay 1 second @10:07:51.84 AS.reconfigure ASfast @10:07:51.91 AS.seek 0ml @10:08:19.28 delay 3 seconds @10:08:22.31 AFV.select :carrierSolution @10:08:22.43 delay 2 seconds @10:08:24.46 AS.reconfigure ASconfig @10:08:24.54 AS.seek 1ml @10:09:22.31 delay 1 second @10:09:23.33 AFV.select :holdingCoil @10:09:23.45 delay 1 second @10:09:24.48 AS.reconfigure ASfast @10:09:24.55 AS.seek 0ml @10:09:51.92 delay 3 seconds @10:09:54.95 AFV.select :carrierSolution @10:09:55.07 delay 2 seconds @10:09:57.11 AS.reconfigure ASconfig @10:09:57.18 AS.seek 1ml @10:10:54.95 delay 1 second @10:10:55.97 AFV.select :holdingCoil @10:10:56.09 delay 1 second @10:10:57.12 AS.reconfigure ASnorm @10:10:57.19 AS.seek 0ml @10:11:47.93 Setting up PCR for IPc -- writing /var/log/esp/IPc20may0210h.pcr @10:11:47.95 Building PCR Slug from elutionWater with MM3 mix, PP6 primer @10:11:47.98 Priming with carrier @10:11:48.00 ATV.dial ARV @10:11:48.64 ARV.dial :carrier @10:11:49.01 AS.seek 0.5ml @10:12:14.57 AFV.select :carrierSolution @10:12:14.71 delay 2 seconds @10:12:16.74 AS.reconfigure ASconfig @10:12:16.82 AS.seek 1ml @10:12:45.85 delay 1 second @10:12:46.87 AFV.select :holdingCoil @10:12:47.58 delay 1 second @10:12:48.61 ATV.dial :waste @10:12:50.31 AS.reconfigure ASnorm @10:12:50.39 AS.seek :empty @10:13:41.16 AFV.select :carrierSolution @10:13:41.29 delay 2 seconds @10:13:43.32 AS.reconfigure ASconfig @10:13:43.39 AS.seek 0.5ml @10:14:12.53 delay 1 second @10:14:13.55 AFV.select :holdingCoil @10:14:13.67 Building Slug @10:14:13.68 ATV.dial :ARV @10:14:15.36 delay 2 seconds @10:14:17.39 ARV.dial :air1 @10:14:17.81 delay 2 seconds @10:14:19.89 AS.reconfigure ASslow2 @10:14:19.97 AS.seek 0.506ml @10:14:26.27 delay 2 seconds @10:14:28.29 ARV.dial :MM3 @10:14:29.36 delay 2 seconds @10:14:31.39 Adding MM3 @10:14:31.49 AS.seek 0.524ml @10:14:49.86 delay 25 seconds @10:15:14.89 ARV.dial :PP6 @10:15:15.31 Adding PP6 @10:15:15.33 delay 2 seconds @10:15:17.42 AS.seek 0.53ml @10:15:23.85 delay 10 seconds @10:15:33.88 ARV.dial :air1 @10:15:34.72 adding ARV air @10:15:34.74 delay 2 seconds @10:15:36.84 AS.seek 0.536ml @10:15:43.06 delay 5 seconds @10:15:48.09 ARV.dial :carrier @10:15:48.52 delay 7 seconds @10:15:55.61 AS.seek 0.594ml @10:16:54.11 delay 5 seconds @10:16:59.14 ATV.dial :elutionWater @10:17:00.60 delay 2 seconds @10:17:02.69 AS.seek 0.6ml @10:17:09.14 delay 4 seconds @10:17:13.17 ATV.dial ARV @10:17:14.62 delay 2 seconds @10:17:16.65 ARV.dial :carrier @10:17:16.67 delay 2 seconds @10:17:18.77 AS.seek 0.65ml @10:18:09.36 delay 4 seconds @10:18:13.39 ATV.dial PCR @10:18:14.28 ASV.dial :waste1 @10:18:14.30 ARV.dial :air1 @10:18:14.74 Positioning Slug by 0.324ml @10:18:14.85 AS.seek 0.326ml @10:23:41.30 delay 5 seconds @10:23:46.33 ATV.dial ARV @10:23:47.11 ASV.dial :PCRlock @10:23:47.52 delay 5 seconds @10:23:52.55 Running IPc ProfileIPc thermocycling @10:23:52.56 PCR.program ProfileIPc @10:23:52.69 ProfileIPc should complete in < 1:40:53 @10:23:52.71 PCR.run 2:11:06.25 @11:46:27.51 PCR.off @11:46:28.11 PCR for IPc completed -- closed /var/log/esp/IPc20may0210h.pcr @11:46:28.19 Cleaning PCR @11:46:28.20 AFV.select :carrierSolution @11:46:28.34 delay 2 seconds @11:46:30.37 AS.reconfigure ASconfig @11:46:30.44 AS.seek 0.5ml @11:46:40.77 delay 1 second @11:46:41.79 AFV.select :holdingCoil @11:46:41.91 ASV.dial :waste1 @11:46:42.30 ATV.dial :air3 @11:46:43.62 AS.reconfigure ASslow1 @11:46:43.69 AS.seek 0.51ml @11:46:46.01 delay 2 seconds @11:46:48.03 ATV.dial :bleach @11:46:48.68 AS.seek 0.81ml @11:47:49.27 delay 3 seconds @11:47:52.30 ATV.dial :air3 @11:47:52.88 delay 3 seconds @11:47:55.97 AS.seek 0.835ml @11:48:01.29 delay 3 seconds @11:48:04.32 ATV.dial :bleach @11:48:04.98 AS.seek 0.82ml @11:48:08.32 delay 3 seconds @11:48:11.34 ARV.dial :waste @11:48:11.97 delay 3 seconds @11:48:15.00 ATV.dial :ARV @11:48:15.91 AS.reconfigure ASconfig @11:48:15.98 AS.seek 0.47ml @11:48:36.37 AFV.select :carrierSolution @11:48:36.51 delay 2 seconds @11:48:38.54 AS.seek 1ml @11:49:09.35 delay 1 second @11:49:10.37 AFV.select :holdingCoil @11:49:10.49 delay 1 second @11:49:11.52 AS.reconfigure ASfast @11:49:11.59 AS.seek 0ml @11:49:38.95 AFV.select :carrierSolution @11:49:39.09 delay 2 seconds @11:49:41.12 AS.reconfigure ASconfig @11:49:41.19 AS.seek 1ml @11:50:38.96 delay 1 second @11:50:39.98 AFV.select :holdingCoil @11:50:40.10 delay 1 second @11:50:41.13 AS.reconfigure ASfast @11:50:41.20 AS.seek 0ml @11:51:08.56 AFV.select :carrierSolution @11:51:08.70 delay 2 seconds @11:51:10.73 AS.reconfigure ASconfig @11:51:10.80 AS.seek 1ml @11:52:08.58 delay 1 second @11:52:09.60 AFV.select :holdingCoil @11:52:09.72 delay 1 second @11:52:10.75 AS.reconfigure ASfast @11:52:10.82 AS.seek 0ml @11:52:38.18 ARV.dial between carrier and air1 @11:52:38.69 ATV.dial :air3 @11:52:40.00 AS.reconfigure ASslow1 @11:52:40.08 AS.seek 0.01ml @11:52:42.47 delay 2 seconds @11:52:44.49 ATV.dial :bleach @11:52:45.72 AS.seek 0.81ml @11:55:26.89 delay 3 seconds @11:55:29.92 ATV.dial :air3 @11:55:30.50 delay 3 seconds @11:55:33.59 AS.seek 0.835ml @11:55:38.90 delay 3 seconds @11:55:41.93 ATV.dial :bleach @11:55:42.59 AS.seek 0.82ml @11:55:46.02 delay 3 seconds @11:55:49.05 ATV.dial PCR @11:55:49.46 AS.reconfigure ASconfig @11:55:49.53 AS.seek :empty @11:56:37.05 PCR.program :bleachProfile @11:56:37.12 PCR program should complete in < 2:45 @11:56:37.13 PCR.run 10:00 @11:59:32.91 PCR.off @11:59:32.95 AFV.select :carrierSolution @11:59:33.07 delay 2 seconds @11:59:35.11 AS.seek 1ml @12:00:32.96 delay 1 second @12:00:33.98 AFV.select :holdingCoil @12:00:34.10 delay 1 second @12:00:35.13 AS.reconfigure ASfast @12:00:35.20 AS.seek 0ml @12:01:02.57 delay 3 seconds @12:01:05.60 AFV.select :carrierSolution @12:01:05.72 delay 2 seconds @12:01:07.75 AS.reconfigure ASconfig @12:01:07.83 AS.seek 1ml @12:02:05.69 delay 1 second @12:02:06.71 AFV.select :holdingCoil @12:02:06.83 delay 1 second @12:02:07.86 AS.reconfigure ASfast @12:02:07.93 AS.seek 0ml @12:02:35.30 delay 3 seconds @12:02:38.33 AFV.select :carrierSolution @12:02:38.45 delay 2 seconds @12:02:40.48 AS.reconfigure ASconfig @12:02:40.56 AS.seek 1ml @12:03:38.36 delay 1 second @12:03:39.38 AFV.select :holdingCoil @12:03:39.50 delay 1 second @12:03:40.53 AS.reconfigure ASfast @12:03:40.60 AS.seek 0ml @12:04:07.94 delay 3 seconds @12:04:10.97 AFV.select :carrierSolution @12:04:11.09 delay 2 seconds @12:04:13.12 AS.reconfigure ASconfig @12:04:13.19 AS.seek 1ml @12:05:10.97 delay 1 second @12:05:11.99 AFV.select :holdingCoil @12:05:12.11 delay 1 second @12:05:13.14 AS.reconfigure ASfast @12:05:13.21 AS.seek 0ml @12:05:40.58 delay 3 seconds @12:05:43.61 AFV.select :carrierSolution @12:05:43.73 delay 2 seconds @12:05:45.76 AS.reconfigure ASconfig @12:05:45.84 AS.seek 1ml @12:06:43.60 delay 1 second @12:06:44.62 AFV.select :holdingCoil @12:06:44.74 delay 1 second @12:06:45.77 AS.reconfigure ASnorm @12:06:45.84 AS.seek 0ml @12:07:36.59 Setting up PCR for IPc2 -- writing /var/log/esp/IPc220may0212h.pcr @12:07:36.61 Building PCR Slug from elutionWater with MM2 mix, PP3 primer @12:07:36.64 Priming with carrier @12:07:36.66 ATV.dial ARV @12:07:37.30 ARV.dial :carrier @12:07:37.68 AS.seek 0.5ml @12:08:03.29 AFV.select :carrierSolution @12:08:03.43 delay 2 seconds @12:08:05.46 AS.reconfigure ASconfig @12:08:05.54 AS.seek 1ml @12:08:34.57 delay 1 second @12:08:35.59 AFV.select :holdingCoil @12:08:35.71 delay 1 second @12:08:36.74 ATV.dial :waste @12:08:38.43 AS.reconfigure ASnorm @12:08:38.51 AS.seek :empty @12:09:29.26 AFV.select :carrierSolution @12:09:29.40 delay 2 seconds @12:09:31.43 AS.reconfigure ASconfig @12:09:31.50 AS.seek 0.5ml @12:10:00.64 delay 1 second @12:10:01.66 AFV.select :holdingCoil @12:10:01.78 Building Slug @12:10:01.80 ATV.dial :ARV @12:10:03.47 delay 2 seconds @12:10:05.50 ARV.dial :air1 @12:10:05.92 delay 2 seconds @12:10:08.01 AS.reconfigure ASslow2 @12:10:08.08 AS.seek 0.506ml @12:10:14.38 delay 2 seconds @12:10:16.40 ARV.dial :MM2 @12:10:17.91 delay 2 seconds @12:10:19.94 Adding MM2 @12:10:20.04 AS.seek 0.524ml @12:10:38.50 delay 25 seconds @12:11:03.53 ARV.dial :PP3 @12:11:04.75 Adding PP3 @12:11:04.77 delay 2 seconds @12:11:06.93 AS.seek 0.53ml @12:11:13.30 delay 10 seconds @12:11:23.33 ARV.dial :air2 @12:11:23.75 adding ARV air @12:11:23.77 delay 2 seconds @12:11:25.86 AS.seek 0.536ml @12:11:32.25 delay 5 seconds @12:11:37.28 ARV.dial :carrier @12:11:38.58 delay 7 seconds @12:11:45.68 AS.seek 0.594ml @12:12:44.33 delay 5 seconds @12:12:49.36 ATV.dial :elutionWater @12:12:50.83 delay 2 seconds @12:12:52.92 AS.seek 0.6ml @12:12:59.14 delay 4 seconds @12:13:03.17 ATV.dial ARV @12:13:04.61 delay 2 seconds @12:13:06.64 ARV.dial :carrier @12:13:06.66 delay 2 seconds @12:13:08.77 AS.seek 0.65ml @12:13:59.30 delay 4 seconds @12:14:03.33 ATV.dial PCR @12:14:04.11 ASV.dial :waste1 @12:14:04.13 ARV.dial :air1 @12:14:04.56 Positioning Slug by 0.324ml @12:14:04.67 AS.seek 0.326ml @12:19:30.66 delay 5 seconds @12:19:35.69 ATV.dial ARV @12:19:36.49 ASV.dial :PCRlock @12:19:36.90 delay 5 seconds @12:19:41.93 Running IPc2 ProfileIPc thermocycling @12:19:41.94 PCR.program ProfileIPc @12:19:42.06 ProfileIPc should complete in < 1:40:53 @12:19:42.08 PCR.run 2:11:06.25 @13:42:16.08 PCR.off @13:42:16.68 PCR for IPc2 completed -- closed /var/log/esp/IPc220may0212h.pcr @13:42:16.77 Cleaning PCR @13:42:16.78 AFV.select :carrierSolution @13:42:16.92 delay 2 seconds @13:42:18.95 AS.reconfigure ASconfig @13:42:19.02 AS.seek 0.5ml @13:42:29.37 delay 1 second @13:42:30.39 AFV.select :holdingCoil @13:42:30.51 ASV.dial :waste1 @13:42:30.91 ATV.dial :air3 @13:42:32.23 AS.reconfigure ASslow1 @13:42:32.30 AS.seek 0.51ml @13:42:34.61 delay 2 seconds @13:42:36.63 ATV.dial :bleach @13:42:37.28 AS.seek 0.81ml @13:43:37.90 delay 3 seconds @13:43:40.93 ATV.dial :air3 @13:43:41.51 delay 3 seconds @13:43:44.60 AS.seek 0.835ml @13:43:49.92 delay 3 seconds @13:43:52.95 ATV.dial :bleach @13:43:53.61 AS.seek 0.82ml @13:43:56.94 delay 3 seconds @13:43:59.97 ARV.dial :waste @13:44:00.60 delay 3 seconds @13:44:03.63 ATV.dial :ARV @13:44:04.53 AS.reconfigure ASconfig @13:44:04.61 AS.seek 0.47ml @13:44:25.01 AFV.select :carrierSolution @13:44:25.15 delay 2 seconds @13:44:27.18 AS.seek 1ml @13:44:58.04 delay 1 second @13:44:59.06 AFV.select :holdingCoil @13:44:59.18 delay 1 second @13:45:00.21 AS.reconfigure ASfast @13:45:00.28 AS.seek 0ml @13:45:27.63 AFV.select :carrierSolution @13:45:27.76 delay 2 seconds @13:45:29.79 AS.reconfigure ASconfig @13:45:29.86 AS.seek 1ml @13:46:27.63 delay 1 second @13:46:28.65 AFV.select :holdingCoil @13:46:28.77 delay 1 second @13:46:29.80 AS.reconfigure ASfast @13:46:29.87 AS.seek 0ml @13:46:57.24 AFV.select :carrierSolution @13:46:57.37 delay 2 seconds @13:46:59.40 AS.reconfigure ASconfig @13:46:59.47 AS.seek 1ml @13:47:57.27 delay 1 second @13:47:58.29 AFV.select :holdingCoil @13:47:58.41 delay 1 second @13:47:59.44 AS.reconfigure ASfast @13:47:59.51 AS.seek 0ml @13:48:26.87 ARV.dial between carrier and air1 @13:48:27.38 ATV.dial :air3 @13:48:28.69 AS.reconfigure ASslow1 @13:48:28.77 AS.seek 0.01ml @13:48:31.12 delay 2 seconds @13:48:33.14 ATV.dial :bleach @13:48:33.80 AS.seek 0.81ml @13:51:14.96 delay 3 seconds @13:51:17.99 ATV.dial :air3 @13:51:18.57 delay 3 seconds @13:51:21.66 AS.seek 0.835ml @13:51:27.01 delay 3 seconds @13:51:30.04 ATV.dial :bleach @13:51:30.70 AS.seek 0.82ml @13:51:34.01 delay 3 seconds @13:51:37.03 ATV.dial PCR @13:51:37.44 AS.reconfigure ASconfig @13:51:37.50 AS.seek :empty @13:52:24.98 PCR.program :bleachProfile @13:52:25.05 PCR program should complete in < 2:45 @13:52:25.06 PCR.run 10:00 @13:55:21.42 PCR.off @13:55:21.46 AFV.select :carrierSolution @13:55:21.58 delay 2 seconds @13:55:23.62 AS.seek 1ml @13:56:21.47 delay 1 second @13:56:22.49 AFV.select :holdingCoil @13:56:22.61 delay 1 second @13:56:23.64 AS.reconfigure ASfast @13:56:23.71 AS.seek 0ml @13:56:51.07 delay 3 seconds @13:56:54.10 AFV.select :carrierSolution @13:56:54.22 delay 2 seconds @13:56:56.25 AS.reconfigure ASconfig @13:56:56.32 AS.seek 1ml @13:57:54.10 delay 1 second @13:57:55.12 AFV.select :holdingCoil @13:57:55.24 delay 1 second @13:57:56.27 AS.reconfigure ASfast @13:57:56.34 AS.seek 0ml @13:58:23.73 delay 3 seconds @13:58:26.76 AFV.select :carrierSolution @13:58:26.88 delay 2 seconds @13:58:28.91 AS.reconfigure ASconfig @13:58:28.99 AS.seek 1ml @13:59:26.80 delay 1 second @13:59:27.82 AFV.select :holdingCoil @13:59:27.94 delay 1 second @13:59:28.97 AS.reconfigure ASfast @13:59:29.04 AS.seek 0ml @13:59:56.41 delay 3 seconds @13:59:59.44 AFV.select :carrierSolution @13:59:59.56 delay 2 seconds @14:00:01.59 AS.reconfigure ASconfig @14:00:01.68 AS.seek 1ml @14:00:59.48 delay 1 second @14:01:00.50 AFV.select :holdingCoil @14:01:00.62 delay 1 second @14:01:01.65 AS.reconfigure ASfast @14:01:01.72 AS.seek 0ml @14:01:29.08 delay 3 seconds @14:01:32.11 AFV.select :carrierSolution @14:01:32.23 delay 2 seconds @14:01:34.26 AS.reconfigure ASconfig @14:01:34.33 AS.seek 1ml @14:02:32.10 delay 1 second @14:02:33.12 AFV.select :holdingCoil @14:02:33.24 delay 1 second @14:02:34.27 AS.reconfigure ASnorm @14:02:34.34 AS.seek 0ml @14:03:25.13
Asleep after completing 20invasivedeploy assays @14:03:25.15 Mission runtime was 1 day, 1:40:29.229334 @14:03:25.17 GoodNews.email "{1 => 3, 2 => 0}\nCan@14:02:58, 21.0C, 36% humidity, 14.4psia, 12.006V, 0.556A, 0.550A avg, 6.68W",Subject: "Asleep after completing 20invasivedeploy assays" @14:03:34.05 Session Ended @17:03:08.49CEST03-May-20
ESP2 v3.30 I2C Gateway v3.06 for core bus @17:03:10.91 Copyright 2018 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @17:03:11.49 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @17:03:18.57 Polling Can every 10:00 @17:03:20.45 SPE.coast @17:03:20.59 SPE.configure SPEconfig @17:03:20.79 AS.reconfigure ASconfig @17:03:20.88 AS.hold @17:03:20.99 AS.configure ASconfig @17:03:21.20 SS.reconfigure SSconfig @17:03:21.28 SS.hold @17:03:21.37 SS.configure SSconfig @17:03:21.47 CC.reconfigure CCconfig @17:03:21.52 CC.hold @17:03:21.57 CC.configure CCconfig @17:03:21.67 CS.reconfigure CSconfig @17:03:21.71 CS.hold @17:03:21.79 CS.configure CSconfig @17:03:22.46 PC.reconfigure PCconfig @17:03:22.55 PC.hold @17:03:22.67 PC.configure PCconfig @17:03:22.87 PS.configure PSconfig @17:03:23.08 Forearm.reconfigure LooseForearm @17:03:23.12 Forearm.hold @17:03:23.18 Forearm.configure LooseForearm @17:03:23.28 Elbow.reconfigure LooseElbow @17:03:23.33 Elbow.hold @17:03:23.40 Elbow.configure LooseElbow @17:03:23.51 SC.reconfigure SCloose @17:03:23.56 SC.hold @17:03:23.62 SC.configure SCloose @17:03:23.75 SE.reconfigure SEconfig @17:03:23.84 SE.hold @17:03:23.95 SE.configure SEconfig @17:03:24.03 CH.coast @17:03:24.15 CH.configure CHconfig @17:03:24.22 PH.coast @17:03:24.34 PH.configure PHconfig @17:03:24.42 PCR.off @17:03:24.48 PCR.pidtemp= 50,0.1,0,300,25,25 @17:03:24.52 PCR.caltemp= 2.8814,0.0372,0 @17:03:24.56 All dwarf controllers running firmware version 3.75 @17:03:24.74 Loading /home/esp/esp2/mission/20invasivedeploymfbtest.rb mission @17:03:24.82 Preparing to sleep until +0:00:10 @17:03:24.86 GoodNews.email "Starting 20invasivedeploymfbtest w/pucks in tube 2\n{}\nCan@17:03:18, 20.9C, 37% humidity, 14.6psia, 11.937V, 0.764A, 0.764A avg, 9.13W",Subject: "20invasivedeploymfbtest begun" @17:03:24.95 AS.coast @17:03:24.97
Asleep til +0:00:10 @17:03:25.01 Continuing at 17:03 @17:03:25.06 SS.coast @17:03:25.13 CC.coast @17:03:25.17 CS.coast @17:03:25.21 PC.coast @17:03:25.29 Forearm.coast @17:03:25.36 Elbow.coast @17:03:25.46 SC.coast @17:03:25.56 SE.coast @17:03:26.06 Session Begun @17:03:26.26 Gate.power :core,:OFF @17:03:26.28 Gate.power :raw,:OFF @17:03:26.30 Shutdown @17:03:32.08 -> showlog 100 @17:03:33.78 GoodNews.email "Then, in tube 2: mbaPhase\n/home/esp/esp2/mission/20invasivedeploymfbtest.rb:119\n{}\nCan@17:03:25, 20.9C, 37% humidity, 14.6psia, 11.937V, 0.764A, 0.764A avg, 9.13W",Subject: "Asleep until 17:03" @17:03:34.82
Powering core bus @17:03:39.80 Gate.power :raw,:ON @17:03:41.70 SPE.coast @17:03:41.89 SPE.configure SPEconfig @17:03:42.14 AS.configure ASconfig @17:03:42.50 SS.configure SSconfig @17:03:42.92 CC.configure CCconfig @17:03:43.33 CS.configure CSconfig @17:03:43.79 PC.configure PCconfig @17:03:44.25 PS.configure PSconfig @17:03:44.79 Forearm.configure LooseForearm @17:03:45.21 Elbow.configure LooseElbow @17:03:45.69 SC.configure SCloose @17:03:46.11 SE.configure SEconfig @17:03:46.30 CH.coast @17:03:46.52 CH.configure CHconfig @17:03:46.68 PH.coast @17:03:46.97 PH.configure PHconfig @17:03:47.13 PCR.off @17:03:47.26 PCR.pidtemp= 50,0.1,0,300,25,25 @17:03:47.40 PCR.caltemp= 2.8814,0.0372,0 @17:03:47.59 All dwarf controllers running firmware version 3.75 @17:03:47.88 Valve.reset @17:03:48.32 PCR.off @17:03:48.45 PCR.pidtemp= 50,0.1,0,300,25,25 @17:03:48.57 PCR.caltemp= 2.8814,0.0372,0 @17:03:49.15 PSV.dial :air @17:03:49.24 Intake.select :close @17:03:49.37 Exhaust.select :close @17:03:49.54 CTV.dial :air @17:03:50.02 CSV.dial :waste1 @17:03:50.17 PS.reconfigure PSconfig @17:03:50.64 PS.home.seek 0ml @17:03:52.07 SSV.dial :spit,avoiding: :exhaust @17:03:52.41
Forearm.reconfigure LooseForearm @17:03:52.78 Forearm.home.seek :retract @17:03:53.32 CS.reconfigure CSfast @17:03:53.42 SS.reconfigure SSconfig @17:03:53.85 CS.home.seek 0ml @17:03:53.95 SS.home.seek 0ml @17:03:54.28 Hand.close @17:03:54.41
Elbow.jog 1500 @17:03:55.19 Elbow.reconfigure LooseElbow @17:03:55.42 Elbow.home.seek :clear @17:04:00.03 PS.coast @17:04:00.47
SE.reconfigure SEconfig @17:04:00.68 SE.home.seek :down @17:04:01.59 SC.reconfigure SCsloppy @17:04:01.78 SC.home.seek :initial @17:04:12.21 Forearm.seek :retract @17:04:12.52 Elbow.seek :hammer @17:04:13.88 Forearm.reconfigure TightForearm @17:04:14.10 Forearm.seek 5 + 350 counts @17:04:15.52 Forearm.seek :retract @17:04:17.52 PC.reconfigure PCconfig @17:04:17.62
CC.reconfigure CCconfig @17:04:17.96 PC.home.seek :open @17:04:18.07
CC.home.seek :open @17:04:22.46 Phase [mbaPhase] @17:04:22.52 GoodNews.email "At 17:04:22 began in tube 2: mbaPhase\n/home/esp/esp2/mission/20invasivedeploymfbtest.rb:119\n{}\nCan@17:04:22, 20.9C, 37% humidity, 14.6psia, 11.942V, 0.752A, 0.746A avg, 8.98W",Subject: "Started 20invasivedeploymfbtest phase mbaPhase" @17:04:22.64CEST03-May-20
===> STARTED Superphase (phase #1) <=== Everybody has a plan untill they get punched in the face - Mike Tyson @17:04:23.04 Starting mfb @17:04:23.09 pcrBAC sampling at most 1500ml, wcr at most 1500ml @17:04:23.35 Loading sh1 puck from tube 2 into Collection Clamp @17:04:23.83 Readying the MFB @17:04:23.89
Hand.open @17:04:24.00 Homing MFB Syringe @17:04:24.13 AFV.select :holdingCoil @17:04:24.56 ATV.dial :air2 @17:04:26.19
SC.reconfigure SCloose @17:04:26.31 AS.reconfigure ASconfig @17:04:27.06 AS.home.seek :home @17:04:27.98
Elbow.reconfigure TightElbow @17:04:28.32 Elbow.seek 2 @17:04:29.44 Forearm.seek 2 + 700 counts @17:04:30.98 SE.reconfigure SEpick @17:04:31.31 SE.seek beamBreak @17:04:31.93 BACfans.email "Started mfb in tube 2 at 17:04:23CEST03-May-20\n{}\nCan@17:04:22, 20.9C, 37% humidity, 14.6psia, 11.942V, 0.752A, 0.746A avg, 8.98W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" @17:04:33.00 AFV.select :carrierSolution @17:04:33.46 delay 2 seconds @17:04:35.20
SE.configure SEconfig @17:04:36.27 AS.seek 1ml @17:04:36.89
Elbow.seek 2 @17:04:38.35 Forearm.seek 2 + 300 counts @17:04:38.84 Forearm.reconfigure LooseForearm @17:04:39.12 Hand.close @17:04:40.24 SE.seek :down @17:04:41.21
Forearm.seek :retract @17:04:42.37 Elbow.seek CC @17:04:43.34 SE.coast @17:04:44.41
Elbow.reconfigure LooseElbow @17:04:44.85 Forearm.reconfigure TightForearm @17:04:45.21 Forearm.seek CC @17:04:47.79 CC.seek :closed @17:05:11.26 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.45 @17:05:11.31 Hand.open @17:05:11.68 I2C::UnexpectedReply in core -- I2C::Servo::Status3Reply[6F:27->20] @17:05:11.76 I2C::UnexpectedReply in core -- I2C::Servo::Status3Reply[6F:27->20] @17:05:12.46 Forearm.reconfigure LooseForearm @17:05:12.67 Forearm.seek :retract @17:05:13.90 SC.coast @17:05:14.10 collectSample 1500ml w/bubble point of 55psi @17:05:14.14 Priming 7ml @17:05:14.20 Intake.select :close @17:05:14.29 Exhaust.select :close @17:05:14.40 SS.hold @17:05:15.17 Calibrating pressure and temperature sensors @17:05:15.23 CTV.dial :air @17:05:15.32 SSV.dial :spit,avoiding: :exhaust @17:05:15.44 SS.reconfigure SSfast @17:05:15.71 SS.seek 5ml @17:05:18.75 SSV.dial CBV @17:05:18.84
CBV.dial SSV @17:05:20.60 delay 3.8 seconds @17:05:24.79 SSV.dial :spit @17:05:26.52 SS.seek 0ml @17:05:29.51 SSV.dial CBV @17:05:29.59 delay 1 second @17:05:30.72 AFV.select :holdingCoil @17:05:30.95 delay 1 second @17:05:31.32
CTV.dial :intake @17:05:32.00 ATV.dial :waste @17:05:32.40 SSV.dial CBV @17:05:32.49
CBV.dial SSV @17:05:32.56 Allowing 40s for 7.0ml of remaining 7.0ml @17:05:32.65 Intake.select :open @17:05:33.51 AS.reconfigure ASnorm @17:05:33.73 AS.home.seek :empty @17:05:35.11
Intake @16.0psia, vacuum from 9.5 to 13.5psi @17:05:35.18 SS.reconfigure SSinhale.with maxSpeed: 150 @17:05:35.42 SS.seek 7ml @17:05:41.64 Stroke completed -- waiting for pressure to equalize ... @17:05:58.22 Intake.select :close @17:05:58.40 SSV.dial :exhaust @17:05:59.31 Exhaust.select :open @17:05:59.44 SS.reconfigure SSexhale @17:05:59.52 SS.seek 0ml @17:06:03.94 Exhaust.select :close @17:06:04.08 Sampling 1500.0ml @17:06:04.10 Intake.select :close @17:06:04.12 Exhaust.select :close @17:06:04.15 SS.reconfigure SSconfig @17:06:04.23 SS.hold @17:06:04.46 CTV.dial :intake @17:06:04.49 SSV.dial CBV @17:06:04.52
CBV.dial SSV @17:06:05.43 Allowing 130s for 25.0ml of remaining 1500.0ml @17:06:05.44 Intake.select :open @17:06:07.65 Intake @16.0psia, vacuum from 9.5 to 13.5psi @17:06:07.68 SS.reconfigure SSinhale.with maxSpeed: 150 @17:06:07.77 SS.seek 25ml @17:06:11.09 Vacuum too high -- waiting up to 40s @17:06:16.60 Intake @16.2psia, vacuum from 9.7 to 13.7psi @17:06:16.62 SS.reconfigure SSinhale.with maxSpeed: 135 @17:06:16.71 SS.seek 25ml @17:06:19.90 Vacuum too high -- waiting up to 40s @17:06:25.57 Intake @16.8psia, vacuum from 10.3 to 14.3psi @17:06:25.60 SS.reconfigure SSinhale.with maxSpeed: 122 @17:06:25.68 SS.seek 25ml @17:06:26.43 ATV.dial :waste @17:06:26.45 ARV.dial between carrier and air1 @17:06:26.57 ASV.dial :waste1 @17:06:28.98
Vacuum too high -- waiting up to 40s @17:06:33.45 Intake @17.1psia, vacuum from 10.6 to 14.6psi @17:06:33.48 SS.reconfigure SSinhale.with maxSpeed: 110 @17:06:33.57 SS.seek 25ml @17:06:36.69 Vacuum too high -- waiting up to 40s @17:06:42.07 Intake @17.2psia, vacuum from 10.7 to 14.7psi @17:06:42.09 SS.reconfigure SSinhale.with maxSpeed: 99 @17:06:42.17 SS.seek 25ml @17:06:45.02 Vacuum too high -- waiting up to 40s @17:06:50.15 Intake @17.5psia, vacuum from 11.0 to 15.0psi @17:06:50.17 SS.reconfigure SSinhale.with maxSpeed: 89 @17:06:50.26 SS.seek 25ml @17:06:53.06 Vacuum too high -- waiting up to 40s @17:06:56.95 Intake @17.1psia, vacuum from 10.6 to 14.6psi @17:06:56.97 SS.reconfigure SSinhale.with maxSpeed: 80 @17:06:57.06 SS.seek 25ml @17:06:59.90 Vacuum too high -- waiting up to 40s @17:07:04.26 Intake @17.3psia, vacuum from 10.8 to 14.8psi @17:07:04.28 SS.reconfigure SSinhale.with maxSpeed: 72 @17:07:04.37 SS.seek 25ml @17:07:07.12 Vacuum too high -- waiting up to 40s @17:07:10.70 Intake @17.2psia, vacuum from 10.7 to 14.7psi @17:07:10.73 SS.reconfigure SSinhale.with maxSpeed: 65 @17:07:10.81 SS.seek 25ml @17:07:13.62 Vacuum too high -- waiting up to 40s @17:07:16.97 Intake @17.3psia, vacuum from 10.8 to 14.8psi @17:07:16.99 SS.reconfigure SSinhale.with maxSpeed: 59 @17:07:17.08 SS.seek 25ml @17:07:19.05 Stroke completed -- waiting for pressure to equalize ... @17:07:22.90 Intake.select :close @17:07:23.09 SSV.dial :exhaust @17:07:24.00 Exhaust.select :open @17:07:24.14 SS.reconfigure SSexhale @17:07:24.22 SS.seek 0ml @17:07:33.38 Exhaust.select :close @17:07:33.52 SSV.dial CBV @17:07:33.55
CBV.dial SSV @17:07:34.48 Allowing 130s for 25.0ml of remaining 1475.0ml @17:07:34.49 Intake.select :open @17:07:36.70 Intake @16.4psia, vacuum from 9.9 to 13.9psi @17:07:36.72 SS.reconfigure SSinhale.with maxSpeed: 59 @17:07:36.81 SS.seek 25ml @17:07:41.96 Vacuum too high -- waiting up to 40s @17:07:46.04 Intake @16.6psia, vacuum from 10.1 to 14.1psi @17:07:46.07 SS.reconfigure SSinhale.with maxSpeed: 53 @17:07:46.16 SS.seek 25ml @17:07:49.87 Vacuum too high -- waiting up to 40s @17:07:53.20 Intake @16.7psia, vacuum from 10.2 to 14.2psi @17:07:53.23 SS.reconfigure SSinhale.with maxSpeed: 48 @17:07:53.31 SS.seek 25ml @17:07:57.40 Vacuum too high -- waiting up to 40s @17:08:01.28 Intake @17.0psia, vacuum from 10.5 to 14.5psi @17:08:01.30 SS.reconfigure SSinhale.with maxSpeed: 43 @17:08:01.39 SS.seek 25ml @17:08:06.52 Vacuum too high -- waiting up to 40s @17:08:10.17 Intake @17.1psia, vacuum from 10.6 to 14.6psi @17:08:10.19 SS.reconfigure SSinhale.with maxSpeed: 39 @17:08:10.28 SS.seek 25ml @17:08:14.71 Vacuum too high -- waiting up to 40s @17:08:17.57 Intake @17.2psia, vacuum from 10.7 to 14.7psi @17:08:17.59 SS.reconfigure SSinhale.with maxSpeed: 35 @17:08:17.68 SS.seek 25ml @17:08:29.15 Vacuum too high -- waiting up to 40s @17:08:34.50 Intake @17.1psia, vacuum from 10.6 to 14.6psi @17:08:34.53 SS.reconfigure SSinhale.with maxSpeed: 32 @17:08:34.62 SS.seek 25ml @17:08:45.96 Vacuum too high -- waiting up to 40s @17:08:50.37 Intake @17.0psia, vacuum from 10.5 to 14.5psi @17:08:50.39 SS.reconfigure SSinhale.with maxSpeed: 29 @17:08:50.48 SS.seek 25ml @17:08:55.08 Stroke completed -- waiting for pressure to equalize ... @17:09:00.17 Intake.select :close @17:09:00.35 SSV.dial :exhaust @17:09:01.25 Exhaust.select :open @17:09:01.37 SS.reconfigure SSexhale @17:09:01.45 SS.seek 0ml @17:09:11.20 Exhaust.select :close @17:09:11.34 SSV.dial CBV @17:09:11.37
CBV.dial SSV @17:09:12.31 Allowing 130s for 25.0ml of remaining 1450.0ml @17:09:12.33 Intake.select :open @17:09:14.53 Intake @16.3psia, vacuum from 9.8 to 13.8psi @17:09:14.55 SS.reconfigure SSinhale.with maxSpeed: 29 @17:09:14.64 SS.seek 25ml @17:09:27.34 Vacuum too high -- waiting up to 40s @17:09:30.42 Intake @16.8psia, vacuum from 10.3 to 14.3psi @17:09:30.44 SS.reconfigure SSinhale.with maxSpeed: 26 @17:09:30.53 SS.seek 25ml @17:09:55.69 Vacuum too high -- waiting up to 40s @17:10:00.04 Intake @16.8psia, vacuum from 10.3 to 14.3psi @17:10:00.06 SS.reconfigure SSinhale.with maxSpeed: 23 @17:10:00.14 SS.seek 25ml @17:10:33.05 Stroke completed -- waiting for pressure to equalize ... @17:10:40.14 Intake.select :close @17:10:40.32 SSV.dial :exhaust @17:10:41.21 Exhaust.select :open @17:10:41.35 SS.reconfigure SSexhale @17:10:41.43 SS.seek 0ml @17:10:50.69 Exhaust.select :close @17:10:50.83 SSV.dial CBV @17:10:50.86
CBV.dial SSV @17:10:51.78 Allowing 130s for 25.0ml of remaining 1425.0ml @17:10:51.80 Intake.select :open @17:10:53.99 Intake @16.3psia, vacuum from 9.8 to 13.8psi @17:10:54.02 SS.reconfigure SSinhale.with maxSpeed: 23 @17:10:54.10 SS.seek 25ml @17:12:08.21 Vacuum too high -- waiting up to 40s @17:12:14.33 Intake @16.6psia, vacuum from 10.1 to 14.1psi @17:12:14.36 SS.reconfigure SSinhale.with maxSpeed: 21 @17:12:14.45 SS.seek 25ml @17:12:17.92 Stroke completed -- waiting for pressure to equalize ... @17:12:22.38 Intake.select :close @17:12:22.56 SSV.dial :exhaust @17:12:23.45 Exhaust.select :open @17:12:23.58 SS.reconfigure SSexhale @17:12:23.65 SS.seek 0ml @17:12:33.39 Exhaust.select :close @17:12:33.53 SSV.dial CBV @17:12:33.56
CBV.dial SSV @17:12:34.46 Allowing 130s for 25.0ml of remaining 1400.0ml @17:12:34.48 Intake.select :open @17:12:36.68 Intake @16.3psia, vacuum from 9.8 to 13.8psi @17:12:36.71 SS.reconfigure SSinhale.with maxSpeed: 21 @17:12:37.29 SS.seek 25ml @17:14:01.19 Stroke completed -- waiting for pressure to equalize ... @17:14:11.37 Intake.select :close @17:14:11.55 SSV.dial :exhaust @17:14:12.45 Exhaust.select :open @17:14:12.58 SS.reconfigure SSexhale @17:14:12.66 SS.seek 0ml @17:14:22.44 Exhaust.select :close @17:14:22.57 SSV.dial CBV @17:14:22.61
CBV.dial SSV @17:14:23.53 Allowing 130s for 25.0ml of remaining 1375.0ml @17:14:23.54 Intake.select :open @17:14:25.75 Intake @16.2psia, vacuum from 9.7 to 13.7psi @17:14:25.77 SS.reconfigure SSinhale.with maxSpeed: 21 @17:14:25.86 SS.seek 25ml @17:15:47.15 Vacuum too high -- waiting up to 40s @17:15:56.42 Intake @16.5psia, vacuum from 10.0 to 14.0psi @17:15:56.54 SS.reconfigure SSinhale.with maxSpeed: 19 @17:15:56.63 SS.seek 25ml @17:15:59.80 Stroke completed -- waiting for pressure to equalize ... @17:16:04.32 Intake.select :close @17:16:04.51 SSV.dial :exhaust @17:16:05.42 Exhaust.select :open @17:16:05.56 SS.reconfigure SSexhale @17:16:05.64 SS.seek 0ml @17:16:15.23 Exhaust.select :close @17:16:15.37 SSV.dial CBV @17:16:15.40
CBV.dial SSV @17:16:16.32 Allowing 130s for 25.0ml of remaining 1350.0ml @17:16:16.33 Intake.select :open @17:16:18.54 Intake @16.2psia, vacuum from 9.7 to 13.7psi @17:16:18.57 SS.reconfigure SSinhale.with maxSpeed: 19 @17:16:18.66 SS.seek 25ml @17:17:51.22 Stroke completed -- waiting for pressure to equalize ... @17:18:06.37 Intake.select :close @17:18:06.55 SSV.dial :exhaust @17:18:07.43 Exhaust.select :open @17:18:07.57 SS.reconfigure SSexhale @17:18:07.65 SS.seek 0ml @17:18:17.42 Exhaust.select :close @17:18:17.56 SSV.dial CBV @17:18:17.59
CBV.dial SSV @17:18:18.51 Allowing 130s for 25.0ml of remaining 1325.0ml @17:18:18.52 Intake.select :open @17:18:20.73 Intake @16.2psia, vacuum from 9.7 to 13.7psi @17:18:20.75 SS.reconfigure SSinhale.with maxSpeed: 19 @17:18:20.84 SS.seek 25ml @17:19:29.68 Vacuum too high -- waiting up to 40s @17:19:40.30 Intake @16.3psia, vacuum from 9.8 to 13.8psi @17:19:40.32 SS.reconfigure SSinhale.with maxSpeed: 17 @17:19:40.41 SS.seek 25ml @17:20:07.45 Stroke completed -- waiting for pressure to equalize ... @17:20:20.03 Intake.select :close @17:20:20.22 SSV.dial :exhaust @17:20:21.12 Exhaust.select :open @17:20:21.25 SS.reconfigure SSexhale @17:20:21.33 SS.seek 0ml @17:20:31.09 Exhaust.select :close @17:20:31.23 SSV.dial CBV @17:20:31.27
CBV.dial SSV @17:20:32.16 Allowing 130s for 25.0ml of remaining 1300.0ml @17:20:32.18 Intake.select :open @17:20:34.37 Intake @16.2psia, vacuum from 9.7 to 13.7psi @17:20:34.39 SS.reconfigure SSinhale.with maxSpeed: 17 @17:20:34.48 SS.seek 25ml @17:22:02.58 Vacuum too high -- waiting up to 40s @17:22:15.58 Intake @16.3psia, vacuum from 9.8 to 13.8psi @17:22:15.62 SS.reconfigure SSinhale.with maxSpeed: 15 @17:22:15.75 SS.seek 25ml @17:22:34.14 Stroke completed -- waiting for pressure to equalize ... @17:22:42.75 SS.reconfigure SSconfig @17:22:42.83 SS.hold @17:22:42.98 Intake.select :close @17:22:43.16 ShallowSampler::Clogged in MAIN -- progress too slow after 225.0ml @17:22:43.18 SSV.dial :exhaust @17:22:44.10 Exhaust.select :open @17:22:44.25 SS.reconfigure SSexhale @17:22:44.32 SS.seek 0ml @17:22:53.94 Exhaust.select :close @17:22:54.06 Sampled 225.0ml @17:22:54.08 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @17:22:54.10 Intake.select :close @17:22:54.21 CSV.dial :waste @17:22:54.31 CS.seek 0ml @17:22:54.41 CSV.dial :air @17:22:54.45 SSV.dial CBV,avoiding: :exhaust @17:22:54.48
CBV.dial SSV @17:22:55.44 CS.seek 10ml @17:22:56.74
CTV.dial :air @17:22:57.68 delay 15 seconds @17:23:12.71 SSV.dial :spit,avoiding: :exhaust @17:23:14.27 CTV.dial CSV,avoiding: :intake @17:23:14.33
SS.seek 0ml @17:23:14.49 SSV.dial CBV,avoiding: :exhaust @17:23:23.46 CBV.dial between SSV and waste2 @17:23:23.92 CTV.dial CSV,avoiding: :air @17:23:23.95
CSV.dial CTV @17:23:24.52 CS.reconfigure CSpressurize @17:23:24.60 CS.seek 0ml @17:23:51.57 CSV.dial :air @17:23:52.16 CS.reconfigure CSfast @17:23:52.24 CS.seek 10ml @17:24:19.46 CSV.dial between air and CTV @17:24:19.87 CS.seek 2.403ml @17:24:40.80 CTV.dial CSV,avoiding: :air @17:24:40.83
CSV.dial CTV @17:24:41.22 CS.reconfigure CSpressurize @17:24:41.34 CS.seek 0ml @17:24:44.48 BubblePoint: Intake @70.8psia, Outlet @15.4psia @17:24:44.49 CBV.dial SSV @17:24:44.91 SS.reconfigure SSconfig @17:24:44.99 SS.seek 10ml @17:24:50.73 delay 15 seconds @17:25:05.87 Evacuated: Intake @38.8psia, Outlet @11.8psia @17:25:05.89 SSV.dial :spit,avoiding: :exhaust @17:25:07.45 SS.reconfigure SSexhale @17:25:07.52 SS.seek 0ml @17:25:13.00 SS.reconfigure SSfast @17:25:13.07 SS.seek 5ml @17:25:15.68 SSV.dial CBV,avoiding: :exhaust @17:25:15.71
CBV.dial SSV @17:25:17.27 CTV.dial :air,avoiding: :intake @17:25:18.91 CSV.dial CTV @17:25:18.97
SS.seek 10ml @17:25:19.95 CSV.dial :air @17:25:20.52 CS.reconfigure CSfast @17:25:20.59 CS.seek 0ml @17:25:21.94
SSV.dial :spit,avoiding: :exhaust @17:25:23.50 SS.reconfigure SSexhale @17:25:23.57 SS.seek 0ml @17:25:25.51 CS.coast @17:25:28.59
SS.reconfigure SSfast @17:25:28.66 SS.seek 5ml @17:25:31.73 SSV.dial CBV,avoiding: :exhaust @17:25:31.77
CBV.dial SSV @17:25:34.32 SSV.dial :spit,avoiding: :exhaust @17:25:35.87 SS.reconfigure SSconfig @17:25:35.95 SS.seek 0ml @17:25:39.01 SS.coast @17:25:39.08 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @17:25:39.09 Intake.select :close @17:25:39.21 CSV.dial :waste @17:25:39.35 SSV.dial CBV,avoiding: :exhaust @17:25:39.38
CBV.dial SSV @17:25:40.65 CS.seek 0ml @17:25:40.74 CSV.dial :air @17:25:40.96
CTV.dial :air @17:25:40.98 delay 15 seconds @17:25:41.66 CS.seek 10ml @17:25:56.02
SSV.dial :spit,avoiding: :exhaust @17:25:57.57 CTV.dial CSV,avoiding: :intake @17:25:57.62
SS.reconfigure SSexhale @17:25:57.75 SS.seek 0ml @17:25:57.83 SSV.dial CBV,avoiding: :exhaust @17:26:09.14 CBV.dial between SSV and waste2 @17:26:09.62 CSV.dial between air and CTV @17:26:10.00 CS.seek 8.023ml @17:26:16.15 CTV.dial CSV,avoiding: :air @17:26:16.18
CSV.dial CTV @17:26:16.69 CS.reconfigure CSpressurize @17:26:16.77 CS.seek 0ml @17:26:38.40 CSV.dial :air @17:26:38.94 CS.reconfigure CSfast @17:26:39.02 CS.seek 10ml @17:27:06.14 CSV.dial between air and CTV @17:27:06.55 CS.seek 3.059ml @17:27:25.66 CTV.dial CSV,avoiding: :air @17:27:25.69
CSV.dial CTV @17:27:26.07 CS.reconfigure CSpressurize @17:27:26.15 CS.seek 0ml @17:27:33.95 BubblePoint: Intake @70.1psia, Outlet @14.9psia @17:27:33.96 CBV.dial SSV @17:27:34.38 SS.reconfigure SSconfig @17:27:34.46 SS.seek 10ml @17:27:40.18 delay 15 seconds @17:27:55.32 Evacuated: Intake @39.2psia, Outlet @17.1psia @17:27:55.33 SSV.dial :spit,avoiding: :exhaust @17:27:56.91 SS.reconfigure SSexhale @17:27:56.99 SS.seek 0ml @17:28:02.44 SS.reconfigure SSfast @17:28:02.52 SS.seek 5ml @17:28:05.41 SSV.dial CBV,avoiding: :exhaust @17:28:05.44
CBV.dial SSV @17:28:07.02 CTV.dial :air,avoiding: :intake @17:28:08.66 CSV.dial CTV @17:28:08.72
SS.seek 10ml @17:28:09.70 CSV.dial :air @17:28:10.27 CS.reconfigure CSfast @17:28:10.34 CS.seek 0ml @17:28:11.40
SSV.dial :spit,avoiding: :exhaust @17:28:12.26 CS.coast @17:28:12.97
SS.reconfigure SSexhale @17:28:13.04 SS.seek 0ml @17:28:18.40 SS.reconfigure SSfast @17:28:18.47 SS.seek 5ml @17:28:21.35 SSV.dial CBV,avoiding: :exhaust @17:28:21.39
CBV.dial SSV @17:28:23.96 SSV.dial :spit,avoiding: :exhaust @17:28:25.51 SS.reconfigure SSconfig @17:28:25.59 SS.seek 0ml @17:28:28.65 SS.coast @17:28:28.71 Lysing: Adding 1.5ml lysis @17:28:28.73 CBV.dial between CSV and waste1,avoiding: :DBV @17:28:30.55 CTV.dial CSV @17:28:32.65 CSV.dial CSR @17:28:33.61 Pulling 1ml of CSR.lysis @17:28:33.62 CSR.select :lysis @17:28:33.79 CS.reconfigure CSconfig @17:28:33.85 CS.seek 1ml @17:28:37.55 delay 5 seconds @17:28:42.57 CSR.select :air @17:28:42.77 CS.seek 6ml @17:28:56.71 delay 2 seconds @17:28:58.73 CSV.dial :waste1 @17:29:00.32 CS.reconfigure CSfast @17:29:00.39 CS.seek :empty @17:29:16.93 CSV.dial CSR @17:29:18.57 Pulling 1.5ml of CSR.lysis @17:29:18.58 CSR.select :lysis @17:29:18.74 CS.reconfigure CSconfig @17:29:18.81 CS.seek 1.5ml @17:29:23.80 delay 5 seconds @17:29:28.82 CSR.select :air @17:29:29.02 CS.seek 4.5ml @17:29:37.87 delay 2 seconds @17:29:39.89 CSV.dial CTV @17:29:40.45 CS.seek 1.275ml @17:29:49.85 Heating sh1 puck from tube 2 in Collection Clamp to 85C for 5:00 @17:29:49.87 CH.seek 85C,8:00 @17:33:35.04 CH.hold 5:00,85C @17:38:35.23 Collection Puck Heater at 85C after lysing @17:38:35.26 CS.seek 0ml @17:38:39.73 CTV.dial between spare and air,avoiding: :intake @17:38:41.01 CSV.dial :air @17:38:41.57 CS.seek 5ml @17:38:55.64 CSV.dial CTV @17:38:56.22 delay 2 seconds @17:38:58.24 CSV.dial :air @17:38:58.81 delay 2 seconds @17:39:00.83 CSV.dial CTV @17:39:01.40 delay 2 seconds @17:39:03.42 CSV.dial :air @17:39:04.00 delay 2 seconds @17:39:06.03 CS.seek 0ml @17:39:20.12 Recovering Lysate into Collection syringe @17:39:20.13 CSV.dial CBV @17:39:21.40 CBV.dial CSV @17:39:21.85 CS.seek 4.999ml @17:39:35.84 delay 5 seconds @17:39:40.87 CTV.dial :air @17:39:41.51 CS.seek 6.999ml @17:39:47.69 delay 5 seconds @17:39:52.72 CSV.dial :air2 @17:39:53.25 CS.seek 10ml @17:40:02.06 CSV.dial between air and CTV,avoiding: CBV @17:40:03.47 Lysing Complete @17:40:03.58 Storing sh1 puck in Collection Clamp to tube 1 @17:40:03.78 SC.reconfigure SCtight @17:40:03.86 SC.seek 1 @17:40:06.03 SC.reconfigure SCloose @17:40:06.28 Elbow.reconfigure TightElbow @17:40:06.35 Elbow.seek 1 @17:40:08.23 Forearm.reconfigure TightForearm @17:40:08.31 Forearm.seek 1 + 700 counts @17:40:09.39 SE.reconfigure SEpick @17:40:09.49 SE.seek beamBreak @17:40:12.58 SE.configure SEconfig @17:40:12.78 Elbow.reconfigure LimpElbow @17:40:12.86 Forearm.reconfigure LooseForearm @17:40:12.92 Forearm.seek 1 @17:40:13.30 Elbow.hold @17:40:13.42 Elbow.reconfigure TightElbow @17:40:13.60 SE.seek 2.07 Pucks below Top Plate @17:40:14.07 Forearm.force -20 @17:40:14.14 Forearm.seek 1 + 330 counts @17:40:14.95 Forearm.reconfigure TightForearm @17:40:15.09 Forearm.seek 1 + 700 counts @17:40:15.41 SE.seek 3.07 Pucks below Top Plate @17:40:16.12 Forearm.reconfigure LooseForearm @17:40:16.19 Forearm.seek :retract @17:40:17.11 Elbow.seek CC @17:40:18.82 Elbow.reconfigure LooseElbow @17:40:18.94 Forearm.seek CC @17:40:20.04 Hand.close @17:40:20.76 CC.seek :open @17:40:25.32 Forearm.seek :retract @17:40:26.37 Elbow.reconfigure TightElbow @17:40:26.44 Elbow.seek 1 @17:40:28.47 Elbow.reconfigure LimpElbow @17:40:28.55 Forearm.seek 1 @17:40:29.60 Elbow.hold @17:40:29.70 Elbow.reconfigure TightElbow @17:40:29.77 Hand.open @17:40:30.65 Forearm.force -20 @17:40:30.72 Forearm.seek 1 + 348 counts @17:40:31.07 Forearm.reconfigure TightForearm @17:40:31.14 Forearm.seek 1 + 700 counts @17:40:31.55 SE.seek :down @17:40:32.71
Forearm.reconfigure LooseForearm @17:40:32.78 Forearm.seek :retract @17:40:34.14 SE.coast @17:40:34.20
SC.coast @17:40:34.26 Tranferring lysate to PRV @17:40:34.27 CSV.dial :air @17:40:34.65 CS.seek 10ml @17:40:34.74 CSV.dial between air and CTV @17:40:35.14 AIV.dial :bypass @17:40:35.36 Beginning samplePassC @17:40:35.38 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:40:35.49 Hand.open @17:40:35.90 Elbow.seek FlushPuck::Garage @17:40:38.03 Elbow.reconfigure LooseElbow @17:40:38.16 Forearm.seek :garage @17:40:39.19 Hand.close @17:40:40.14 Forearm.seek :retract @17:40:41.19 Elbow.reconfigure TightElbow @17:40:41.26 Elbow.seek CC @17:40:44.21 Elbow.reconfigure LooseElbow @17:40:44.34 Forearm.reconfigure TightForearm @17:40:44.41 Forearm.seek CC @17:40:46.32 CC.seek :closed @17:40:50.68 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.50 @17:40:50.77 Hand.open @17:40:51.59 Forearm.reconfigure LooseForearm @17:40:51.66 Forearm.seek :retract @17:40:52.75 AWV.select :air @17:40:52.77 AIV.dial :bypass @17:40:52.80 PSV.dial PSR @17:40:54.40 Pulling 1.5ml of PSR.flush @17:40:54.41 PSR.select :flush @17:40:54.57 PS.seek 1.5ml @17:41:11.01 delay 5 seconds @17:41:16.04 PSV.dial :waste1 @17:41:16.57 PS.seek 0ml @17:41:33.06 PSV.dial PSR @17:41:33.60 PSR.select :air @17:41:33.75 PS.seek 2.5ml @17:42:00.76 PRV.dial CBV @17:42:00.80
CBV.dial PRV @17:42:01.78 PSV.dial :mixloop @17:42:02.95 CTV.dial :air,avoiding: :intake @17:42:02.96 PTV.dial :PRVmixing @17:42:03.10 PS.seek 0ml @17:42:30.07 PSV.dial :air @17:42:30.61 PS.seek 2.5ml @17:42:57.38 PSV.dial :mixloop @17:42:57.92 PS.seek 0ml @17:43:24.78 PSV.dial :air @17:43:25.32 PS.seek 2.5ml @17:43:51.94 PSV.dial :mixloop @17:43:52.49 PS.seek 0ml @17:44:19.14 Garaging FlushPuck in Collection Clamp @17:44:19.24 Elbow.reconfigure TightElbow @17:44:19.31 Elbow.seek CC @17:44:19.59 Elbow.reconfigure LooseElbow @17:44:19.72 Forearm.seek CC @17:44:20.82 Hand.close @17:44:21.64 CC.seek :open @17:44:26.07 Forearm.seek :retract @17:44:27.20 Elbow.reconfigure TightElbow @17:44:27.27 Elbow.seek :garage @17:44:30.48 Elbow.reconfigure LooseElbow @17:44:30.61 Forearm.reconfigure TightForearm @17:44:30.68 Forearm.seek :garage @17:44:31.81 Hand.open @17:44:32.71 Forearm.reconfigure LooseForearm @17:44:32.78 Forearm.seek :retract @17:44:33.85 Connecting PS to CS to transfer sample @17:44:33.86 CBV.dial AIV @17:44:33.89 AIV.dial :bypass @17:44:33.92 PSV.dial :mixloop @17:44:33.94 PTV.dial :PRVmixing @17:44:33.97 PRV.dial AIV @17:44:34.00 CTV.dial :air,avoiding: :intake @17:44:34.02 Loading lyfil puck from tube 2 into Collection Clamp @17:44:34.31 SC.reconfigure SCtight @17:44:34.38 SC.seek 2 @17:44:36.51 SC.reconfigure SCloose @17:44:36.77 Elbow.reconfigure TightElbow @17:44:36.85 Elbow.seek 2 @17:44:39.06 Forearm.reconfigure TightForearm @17:44:39.13 Forearm.seek 2 + 700 counts @17:44:40.16 SE.reconfigure SEpick @17:44:40.26 SE.seek beamBreak @17:44:43.40 SE.configure SEconfig @17:44:43.58 Elbow.seek 2 @17:44:43.81 Forearm.seek 2 + 300 counts @17:44:44.09 Forearm.reconfigure LooseForearm @17:44:44.15 Hand.close @17:44:44.91 SE.seek :down @17:44:45.16
Forearm.seek :retract @17:44:46.14 Elbow.seek CC @17:44:47.72 SE.coast @17:44:47.88
Elbow.reconfigure LooseElbow @17:44:48.00 Forearm.reconfigure TightForearm @17:44:48.08 Forearm.seek CC @17:44:49.44 CC.seek :closed @17:44:53.69 Collection Clamp onSpring/offSpring for 5/31 samples, on/off torque 2.42 @17:44:53.71 Hand.open @17:44:54.59 Forearm.reconfigure LooseForearm @17:44:54.66 Forearm.seek :retract @17:44:55.73 SC.coast @17:44:55.78 CTV.dial CSV,avoiding: :intake @17:44:57.39 CSV.dial CTV @17:44:57.76 CS.seek 5ml @17:45:11.69 delay 1:20 @17:46:31.73 CS.seek 2.5ml @17:46:39.13 delay 40 seconds @17:47:19.16 CS.seek 0ml @17:47:26.69 delay 1:00 @17:48:26.72 PSV.dial :air @17:48:27.26 PS.seek 2.5ml @17:48:53.63 PSV.dial :mixloop @17:48:54.17 delay 3 seconds @17:48:57.20 PSV.dial :air @17:48:57.75 delay 2 seconds @17:48:59.78 PSV.dial :mixloop @17:49:00.29 delay 3 seconds @17:49:03.32 PSV.dial :waste1 @17:49:04.85 PS.seek :empty @17:49:31.60 CBV.dial :waste1 @17:49:32.16 delay 5 seconds @17:49:37.19 CBV.dial AIV @17:49:37.74 delay 10 seconds @17:49:47.77 CBV.dial :waste1 @17:49:48.32 delay 10 seconds @17:49:58.35 CBV.dial AIV @17:49:58.89 delay 10 seconds @17:50:08.92 CTV.dial CSV,avoiding: :intake @17:50:08.93 CBV.dial between AIV and waste1 @17:50:09.33 CSV.dial :air @17:50:09.89 CS.seek 10ml @17:50:36.43 CSV.dial CTV @17:50:36.99 CS.seek 0ml @17:51:04.03 CBV.dial :waste1 @17:51:04.93 delay 10 seconds @17:51:14.96 CTV.dial :air,avoiding: :intake @17:51:16.64 Storing lyfil puck in Collection Clamp to tube 1 @17:51:16.85 SC.reconfigure SCtight @17:51:16.92 SC.seek 1 @17:51:19.07 SC.reconfigure SCloose @17:51:19.32 Elbow.reconfigure TightElbow @17:51:19.39 Elbow.seek 1 @17:51:21.34 Forearm.reconfigure TightForearm @17:51:21.42 Forearm.seek 1 + 700 counts @17:51:22.48 SE.reconfigure SEpick @17:51:22.58 SE.seek beamBreak @17:51:25.54 SE.configure SEconfig @17:51:25.74 Elbow.reconfigure LimpElbow @17:51:25.81 Forearm.reconfigure LooseForearm @17:51:25.88 Forearm.seek 1 @17:51:26.25 Elbow.hold @17:51:26.34 Elbow.reconfigure TightElbow @17:51:26.48 SE.seek 3.08 Pucks below Top Plate @17:51:27.00 Forearm.force -20 @17:51:27.07 Forearm.seek 1 + 316 counts @17:51:27.44 Forearm.reconfigure TightForearm @17:51:27.51 Forearm.seek 1 + 700 counts @17:51:27.90 SE.seek 4.08 Pucks below Top Plate @17:51:28.64 Forearm.reconfigure LooseForearm @17:51:28.71 Forearm.seek :retract @17:51:29.63 Elbow.seek CC @17:51:31.31 Elbow.reconfigure LooseElbow @17:51:31.44 Forearm.seek CC @17:51:32.52 Hand.close @17:51:33.27 CC.seek :open @17:51:37.79 Forearm.seek :retract @17:51:38.86 Elbow.reconfigure TightElbow @17:51:38.93 Elbow.seek 1 @17:51:40.90 Elbow.reconfigure LimpElbow @17:51:40.98 Forearm.seek 1 @17:51:42.00 Elbow.hold @17:51:42.10 Elbow.reconfigure TightElbow @17:51:42.17 Hand.open @17:51:43.03 Forearm.force -20 @17:51:43.09 Forearm.seek 1 + 343 counts @17:51:43.43 Forearm.reconfigure TightForearm @17:51:43.51 Forearm.seek 1 + 700 counts @17:51:43.91 SE.seek :down @17:51:45.06
Forearm.reconfigure LooseForearm @17:51:45.14 Forearm.seek :retract @17:51:46.38 SE.coast @17:51:46.44
SC.coast @17:51:46.50 PSV.dial :air @17:51:47.75 PS.seek 0.499ml @17:51:54.20 delay 3 seconds @17:51:57.22 PSV.dial :mixloop @17:51:57.84 PRV.dial :air @17:51:58.39 delay 3 seconds @17:52:01.42 PRV.dial CBV @17:52:01.95 CBV.dial AIV @17:52:02.52 PRV.dial between CBV and air @17:52:02.92 PRV.dial :air @17:52:03.35 PS.reconfigure PSslow @17:52:03.42 PS.seek 0.599ml @17:52:05.98 PRV.dial :waste2 @17:52:06.84 PS.reconfigure PSconfig @17:52:06.91 PS.seek :empty @17:52:13.92 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:52:14.01 Hand.open @17:52:14.40 Elbow.seek FlushPuck::Garage @17:52:16.28 Elbow.reconfigure LooseElbow @17:52:16.41 Forearm.seek :garage @17:52:17.45 Hand.close @17:52:18.40 Forearm.seek :retract @17:52:19.49 Elbow.reconfigure TightElbow @17:52:19.56 Elbow.seek CC @17:52:22.47 Elbow.reconfigure LooseElbow @17:52:22.60 Forearm.reconfigure TightForearm @17:52:22.67 Forearm.seek CC @17:52:24.04 CC.seek :closed @17:52:28.32 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.39 @17:52:28.41 Hand.open @17:52:29.21 Forearm.reconfigure LooseForearm @17:52:29.28 Forearm.seek :retract @17:52:30.40 Clearing PML for modified lysate @17:52:30.41 CTV.dial :air,avoiding: :intake @17:52:30.45 PRV.dial :waste2 @17:52:30.48 PTV.dial :PRV,avoiding: :puck @17:52:31.33 delay 2 seconds @17:52:33.35 PTV.dial :PRVmixing,avoiding: :puck @17:52:34.20 delay 2 seconds @17:52:36.22 PSV.dial :PML @17:52:36.23 delay 2 seconds @17:52:38.28 PRV.dial :air @17:52:39.12 delay 2 seconds @17:52:41.20 PS.seek 1.499ml @17:52:57.51 delay 5 seconds @17:53:02.54 PS.seek 2.5ml @17:53:13.60 PSV.dial :air @17:53:14.24 PS.seek 0ml @17:53:40.95 mixing lysate with speDil @17:53:40.96 PSV.dial :PML @17:53:41.48 CBV.dial :PRV @17:53:41.49 PRV.dial :air @17:53:41.53 delay 2 seconds @17:53:43.56 PRV.dial :speDil @17:53:44.11 delay 2 seconds @17:53:46.19 PS.reconfigure PSslow1 @17:53:46.26 PS.seek 0.225ml @17:54:09.98 delay 5 seconds @17:54:15.01 PRV.dial CBV @17:54:16.40 delay 2 seconds @17:54:18.48 PS.seek 0.475ml @17:54:44.26 delay 5 seconds @17:54:49.29 PRV.dial :air @17:54:49.84 delay 2 seconds @17:54:51.87 PS.seek 0.965ml @17:55:41.98 delay 10 seconds @17:55:52.01 PRV.dial CBV @17:55:52.56 CTV.dial :air,avoiding: :intake @17:55:52.58 PSV.dial :air @17:55:53.12 PS.reconfigure PSconfig @17:55:53.20 PS.seek 2.5ml @17:56:09.89 delay 3 seconds @17:56:12.91 PSV.dial PTV @17:56:13.78 PTV.dial :PRV,avoiding: :puck @17:56:14.67 PS.seek 0ml @17:56:41.44 PSV.dial :air @17:56:42.32 PS.seek 2.5ml @17:57:08.98 delay 3 seconds @17:57:12.00 PSV.dial PTV @17:57:12.86 PTV.dial :PRV,avoiding: :puck @17:57:12.89 PS.seek 0ml @17:57:39.48 Pushing modified lysate into AIV Injection Loop @17:57:39.49 CBV.dial AIV @17:57:39.52 AIV.dial :bypass @17:57:39.55 PRV.dial CBV @17:57:39.58 PSV.dial :air @17:57:40.46 PS.seek 2.5ml @17:58:07.14 delay 3 seconds @17:58:10.16 PSV.dial :PML @17:58:10.80 PTV.dial :PRVmixing,avoiding: :puck @17:58:11.73 PS.reconfigure PSslow1 @17:58:11.80 PS.seek 1.74ml @17:59:29.36 delay 5 seconds @17:59:34.39 AIV.dial :sample @17:59:35.06 AWV.select :waste @17:59:35.25 PS.reconfigure PSslow2 @17:59:35.32 PS.seek 1.22ml @18:01:22.58 delay 15 seconds @18:01:37.61 AIV.dial :bypass @18:01:38.28 PS.reconfigure PSconfig @18:01:38.36 PS.seek 0ml @18:01:51.63 PSV.dial :air @18:01:52.17 PS.seek 2.5ml @18:02:18.49 PSV.dial :PML @18:02:19.03 PS.seek 0ml @18:02:45.32 AIV.dial :coilToMFB @18:02:45.34 AWV.select :air @18:02:45.55 Extracting Nucleic Acid from AIV @18:02:45.58
Beginning samplePassD @18:02:45.62 SPE.hold 2:46:40,55C @18:02:45.73 ASV.dial :SPEdelivery @18:02:45.83
AWV.select :air @18:02:45.85 AIV.dial :bypass @18:02:45.88 PSV.dial PSR @18:02:46.18 delay 20 seconds @18:02:47.23
Pulling 1.5ml of PSR.killMFB @18:02:47.24 PSR.select :killMFB @18:02:47.40 PS.seek 1.5ml @18:03:04.67 delay 5 seconds @18:03:06.21 primeLysateINT @18:03:06.22 AWV.select :air @18:03:06.25 AIV.dial :coilToMFB @18:03:06.28 ATV.dial AIV @18:03:08.25 AS.reconfigure ASslow1 @18:03:08.32 AS.seek 0.299ml @18:03:09.70
PSV.dial :waste1 @18:03:10.26 PS.seek 0ml @18:03:27.51 PSV.dial PSR @18:03:28.08 PSR.select :air @18:03:28.23 PS.seek 2.5ml @18:03:56.55 PRV.dial CBV @18:03:56.58
CBV.dial PRV @18:03:56.61 PSV.dial :mixloop @18:03:57.88 CTV.dial :air,avoiding: :intake @18:03:57.89 PTV.dial :PRVmixing @18:03:58.46 PS.seek 0ml @18:04:08.96 delay 10 seconds @18:04:18.99 ATV.dial :air2 @18:04:19.94 AS.seek 0.329ml @18:04:26.16 ATV.dial :waste2 @18:04:26.70
PSV.dial PSR @18:04:26.81 AS.reconfigure ASnorm @18:04:26.87 AS.seek :empty @18:04:28.04
Pulling 1.5ml of PSR.flush @18:04:28.05 PSR.select :flush @18:04:28.21 PS.seek 1.5ml @18:04:43.88 Lysate primed! @18:04:43.89 AFV.select :holdingCoil @18:04:43.92 ATV.dial :air2 @18:04:44.63 AS.reconfigure ASslow1 @18:04:44.70 AS.seek 0.069ml @18:04:45.49
delay 5 seconds @18:04:50.52 PSV.dial :waste1 @18:04:51.07 PS.seek 0ml @18:04:59.13 delay 3 seconds @18:05:02.15 ATV.dial :AIV @18:05:03.09 AS.seek 0.469ml @18:05:08.39
PSV.dial PSR @18:05:08.96 PSR.select :air @18:05:09.11 PS.seek 2.5ml @18:05:37.46 PRV.dial CBV @18:05:37.49
CBV.dial PRV @18:05:37.53 PSV.dial :mixloop @18:05:38.78 CTV.dial :air,avoiding: :intake @18:05:38.80 PTV.dial :PRVmixing @18:05:38.85 PS.seek 0ml @18:06:06.52 PSV.dial PSR @18:06:07.94 Pulling 1.5ml of PSR.flush @18:06:07.95 PSR.select :flush @18:06:08.11 PS.seek 1.5ml @18:06:23.81 delay 5 seconds @18:06:25.21
delay 5 seconds @18:06:28.90 Solid Phase Extraction Heater at 48.7C @18:06:28.91 ATV.dial ASV @18:06:29.37 ASV.dial :SPEdelivery @18:06:29.47 AS.seek 0.041ml @18:06:30.24
PSV.dial :waste1 @18:06:30.81 PS.seek 0ml @18:06:47.64 PSV.dial PSR @18:06:48.30 PSR.select :air @18:06:48.45 PS.seek 2.5ml @18:07:15.92 PRV.dial CBV @18:07:15.95
CBV.dial PRV @18:07:15.98 PSV.dial :mixloop @18:07:17.22 CTV.dial :air,avoiding: :intake @18:07:17.24 PTV.dial :PRVmixing @18:07:17.28 PS.seek 0ml @18:07:44.74 PSV.dial PSR @18:07:46.13 Pulling 1.5ml of PSR.flush @18:07:46.14 PSR.select :flush @18:07:46.30 PS.seek 1.5ml @18:07:55.85 delay 10 seconds @18:08:02.77
delay 5 seconds @18:08:05.88 rinseLysateLineINT @18:08:05.89 AFV.select :carrierSolution @18:08:06.02 delay 2 seconds @18:08:07.80
PSV.dial :waste1 @18:08:08.04 AS.reconfigure ASconfig @18:08:08.11 AS.seek 1ml @18:08:08.36
PS.seek 0ml @18:08:24.88 PSV.dial PSR @18:08:25.43 PSR.select :air @18:08:25.58 PS.seek 2.5ml @18:08:52.77 PRV.dial CBV @18:08:52.81
CBV.dial PRV @18:08:52.83 PSV.dial :mixloop @18:08:54.05 CTV.dial :air,avoiding: :intake @18:08:54.06 PTV.dial :PRVmixing @18:08:54.11 PS.reconfigure PSslow @18:08:54.19 PS.seek 1.2ml @18:09:03.57 delay 1 second @18:09:04.58 AFV.select :holdingCoil @18:09:04.70 delay 1 second @18:09:05.73 ATV.dial AIV @18:09:06.42 AIV.dial :coilToMFB @18:09:06.43 AWV.select :waste @18:09:07.02 AS.reconfigure ASslow0 @18:09:07.17 AS.seek 0ml @18:09:21.02
PSV.dial :waste1 @18:09:22.55 PS.reconfigure PSconfig @18:09:22.63 PS.seek 0ml @18:09:36.02 PSV.dial :air @18:09:37.23 PS.seek 2.5ml @18:10:04.05 PSV.dial :mixloop @18:10:04.57 PS.reconfigure PSslow @18:10:04.65 PS.seek 0ml @18:10:48.10 ATV.dial :air2 @18:10:48.81 AS.reconfigure ASslow1 @18:10:48.88 AS.seek 0.05ml @18:10:55.77
PSV.dial :air @18:10:56.30 PS.reconfigure PSconfig @18:10:56.38 PS.seek 2.5ml @18:10:59.22 ATV.dial :AIV @18:11:00.11 AS.seek 0.01ml @18:11:08.46 ATV.dial :waste @18:11:10.20 AS.seek :empty @18:11:12.62 ethanol wash @18:11:12.63 ATV.dial :air2 @18:11:13.97 AS.reconfigure ASslow2 @18:11:14.05 AS.seek 0.009ml @18:11:23.32
PSV.dial :mixloop @18:11:23.86 PS.reconfigure PSslow @18:11:23.94 PS.seek 0ml @18:11:25.04 delay 3 seconds @18:11:28.07 Pulling 0.4ml of Ethanol Wash @18:11:28.08 ATV.dial :ethanol @18:11:28.97 AS.reconfigure ASslow1 @18:11:29.04 AS.seek 0.409ml @18:12:15.08
Cleaning Collection Syringe @18:12:15.09 CSV.dial CSR @18:12:15.70 Pulling 4ml of CSR.flush @18:12:15.71 CSR.select :flush @18:12:15.88 CS.seek 4ml @18:12:27.24 delay 5 seconds @18:12:32.26 CSR.select :air @18:12:32.42 CS.seek 10ml @18:12:48.92 CSV.dial CTV @18:12:48.95
CTV.dial CSV @18:12:49.76 delay 3 seconds @18:12:50.64
CBV.dial :waste1 @18:12:51.23 CS.seek 0ml @18:12:52.78 ATV.dial :air1 @18:12:53.21 delay 3 seconds @18:12:56.30 AS.seek 0.434ml @18:13:01.67 delay 3 seconds @18:13:04.69 ATV.dial :ethanol @18:13:05.36 AS.seek 0.419ml @18:13:08.66 delay 3 seconds @18:13:11.69 ATV.dial ASV @18:13:13.08 AS.seek 0.092ml @18:13:18.36
CSV.dial CSR @18:13:18.97 Pulling 4ml of CSR.flush @18:13:18.98 CSR.select :flush @18:13:19.15 CS.seek 4ml @18:13:30.52 delay 5 seconds @18:13:35.54 CSR.select :air @18:13:35.70 CS.seek 10ml @18:13:52.17 CSV.dial CTV @18:13:52.20
CTV.dial CSV @18:13:52.78 CBV.dial :waste1 @18:13:52.81 CS.seek 0ml @18:14:19.10 delay 3 seconds @18:14:19.68
Garaging FlushPuck in Collection Clamp @18:14:19.78 Elbow.reconfigure TightElbow @18:14:19.85 Elbow.seek CC @18:14:20.12 Elbow.reconfigure LooseElbow @18:14:20.24 Forearm.seek CC @18:14:21.33 Hand.close @18:14:22.05 CC.seek :open @18:14:22.67 ATV.dial :waste2 @18:14:23.48 AS.reconfigure ASconfig @18:14:23.55 AS.seek :empty @18:14:27.03
Forearm.seek :retract @18:14:28.09 Elbow.reconfigure TightElbow @18:14:28.16 Elbow.seek :garage @18:14:29.23 ATV.dial :air2 @18:14:29.65 AS.reconfigure ASslow1 @18:14:29.73 AS.seek 1ml @18:14:31.40
Elbow.reconfigure LooseElbow @18:14:31.53 Forearm.reconfigure TightForearm @18:14:31.61 Forearm.seek :garage @18:14:32.78 Hand.open @18:14:33.62 Forearm.reconfigure LooseForearm @18:14:33.69 Forearm.seek :retract @18:14:34.76 Whole Cell Archive sampling 1500ml @18:14:34.78 Loading wcr puck from tube 2 into Collection Clamp @18:14:35.09 SC.reconfigure SCtight @18:14:35.17 SC.seek 2 @18:14:37.38 SC.reconfigure SCloose @18:14:37.64 Elbow.reconfigure TightElbow @18:14:37.71 Elbow.seek 2 @18:14:39.83 Forearm.reconfigure TightForearm @18:14:39.90 Forearm.seek 2 + 700 counts @18:14:40.94 SE.reconfigure SEpick @18:14:41.05 SE.seek beamBreak @18:14:44.33 SE.configure SEconfig @18:14:44.52 Elbow.seek 2 @18:14:44.76 Forearm.seek 2 + 300 counts @18:14:45.08 Forearm.reconfigure LooseForearm @18:14:45.15 Hand.close @18:14:45.94 SE.seek :down @18:14:46.18 Forearm.seek :retract @18:14:47.17 Elbow.seek CC @18:14:48.88 Elbow.reconfigure LooseElbow @18:14:49.03 SE.coast @18:14:49.05 Forearm.reconfigure TightForearm @18:14:49.17 Forearm.seek CC @18:14:50.55 CC.seek :closed @18:14:54.83 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.40 @18:14:54.84 Hand.open @18:14:55.75 Forearm.reconfigure LooseForearm @18:14:55.83 Forearm.seek :retract @18:14:57.47 SC.coast @18:14:57.53 collectSample 1500ml w/bubble point of 55psi @18:14:57.55 Priming 7ml @18:14:57.57 Intake.select :close @18:14:57.60 Exhaust.select :close @18:14:57.63 SS.hold @18:14:57.89 Calibrating pressure sensors @18:14:57.91 CTV.dial :air @18:14:59.53 SSV.dial :spit,avoiding: :exhaust @18:14:59.56 SS.reconfigure SSfast @18:14:59.65 SS.seek 5ml @18:15:02.38 SSV.dial CBV @18:15:02.42 CBV.dial SSV @18:15:04.13 delay 3.8 seconds @18:15:08.08 SSV.dial :spit @18:15:09.64 SS.seek 0ml @18:15:12.71 SSV.dial CBV @18:15:14.27 CTV.dial :intake @18:15:15.18 SSV.dial CBV @18:15:15.21 CBV.dial SSV @18:15:15.25 Allowing 40s for 7.0ml of remaining 7.0ml @18:15:15.29 Intake.select :open @18:15:17.48 Intake @16.0psia, vacuum from 9.5 to 13.5psi @18:15:17.51 SS.reconfigure SSinhale.with maxSpeed: 150 @18:15:17.60 SS.seek 7ml @18:15:23.00 Stroke completed -- waiting for pressure to equalize ... @18:15:44.03 Intake.select :close @18:15:44.21 SSV.dial :exhaust @18:15:45.12 Exhaust.select :open @18:15:45.25 SS.reconfigure SSexhale @18:15:45.33 SS.seek 0ml @18:15:49.16 Exhaust.select :close @18:15:49.86 Sampling 1500.0ml @18:15:49.88 Intake.select :close @18:15:49.89 Exhaust.select :close @18:15:49.93 SS.reconfigure SSconfig @18:15:50.01 SS.hold @18:15:50.27 CTV.dial :intake @18:15:50.30 SSV.dial CBV @18:15:50.33 CBV.dial SSV @18:15:51.26 Allowing 130s for 25.0ml of remaining 1500.0ml @18:15:51.28 Intake.select :open @18:15:53.50 Intake @15.8psia, vacuum from 9.3 to 13.3psi @18:15:53.53 SS.reconfigure SSinhale.with maxSpeed: 150 @18:15:53.61 SS.seek 25ml @18:15:58.79 Vacuum too high -- waiting up to 40s @18:16:09.71 Intake @16.6psia, vacuum from 10.1 to 14.1psi @18:16:09.74 SS.reconfigure SSinhale.with maxSpeed: 135 @18:16:09.82 SS.seek 25ml @18:16:13.69 Vacuum too high -- waiting up to 40s @18:16:24.44 Intake @16.9psia, vacuum from 10.4 to 14.4psi @18:16:24.46 SS.reconfigure SSinhale.with maxSpeed: 122 @18:16:24.55 SS.seek 25ml @18:16:28.19 Vacuum too high -- waiting up to 40s @18:16:38.40 Intake @16.9psia, vacuum from 10.4 to 14.4psi @18:16:38.42 SS.reconfigure SSinhale.with maxSpeed: 110 @18:16:38.51 SS.seek 25ml @18:16:41.88 Vacuum too high -- waiting up to 40s @18:16:50.48 Intake @16.8psia, vacuum from 10.3 to 14.3psi @18:16:50.51 SS.reconfigure SSinhale.with maxSpeed: 99 @18:16:50.60 SS.seek 25ml @18:16:54.66 Vacuum too high -- waiting up to 40s @18:17:05.11 SS.reconfigure SSinhale.with maxSpeed: 89 @18:17:05.19 SS.seek 25ml @18:17:08.25 Stroke completed -- waiting for pressure to equalize ... @18:17:19.07 Intake.select :close @18:17:19.25 SSV.dial :exhaust @18:17:20.17 Exhaust.select :open @18:17:20.31 SS.reconfigure SSexhale @18:17:20.38 SS.seek 0ml @18:17:29.92 Exhaust.select :close @18:17:30.06 SSV.dial CBV @18:17:30.65 CBV.dial SSV @18:17:31.59 Allowing 130s for 25.0ml of remaining 1475.0ml @18:17:31.62 Intake.select :open @18:17:33.82 Intake @16.0psia, vacuum from 9.5 to 13.5psi @18:17:33.85 SS.reconfigure SSinhale.with maxSpeed: 89 @18:17:33.94 SS.seek 25ml @18:17:38.14 Vacuum too high -- waiting up to 40s @18:17:45.70 Intake @16.4psia, vacuum from 9.9 to 13.9psi @18:17:45.73 SS.reconfigure SSinhale.with maxSpeed: 80 @18:17:45.82 SS.seek 25ml @18:17:49.16 Vacuum too high -- waiting up to 40s @18:17:51.34 delay 5 seconds @18:17:55.73 Intake @16.5psia, vacuum from 10.0 to 14.0psi @18:17:55.75 SS.reconfigure SSinhale.with maxSpeed: 72 @18:17:55.84 SS.seek 25ml @18:17:56.36 ATV.dial ASV @18:17:57.42 ASV.dial :SPEdelivery @18:17:57.45 AS.seek 0.02ml @18:17:59.77 Vacuum too high -- waiting up to 40s @18:18:06.89 Intake @16.5psia, vacuum from 10.0 to 14.0psi @18:18:06.91 SS.reconfigure SSinhale.with maxSpeed: 65 @18:18:07.00 SS.seek 25ml @18:18:11.60 Vacuum too high -- waiting up to 40s @18:18:19.71 Intake @16.6psia, vacuum from 10.1 to 14.1psi @18:18:19.74 SS.reconfigure SSinhale.with maxSpeed: 59 @18:18:19.83 SS.seek 25ml @18:18:24.16 Vacuum too high -- waiting up to 40s @18:18:32.52 Intake @16.4psia, vacuum from 9.9 to 13.9psi @18:18:32.54 SS.reconfigure SSinhale.with maxSpeed: 53 @18:18:32.63 SS.seek 25ml @18:18:38.82 Vacuum too high -- waiting up to 40s @18:18:48.75 SS.reconfigure SSinhale.with maxSpeed: 48 @18:18:48.83 SS.seek 25ml @18:18:54.69 Vacuum too high -- waiting up to 40s @18:19:04.63 Intake @16.4psia, vacuum from 9.9 to 13.9psi @18:19:04.65 SS.reconfigure SSinhale.with maxSpeed: 43 @18:19:04.75 SS.seek 25ml @18:19:06.58 Stroke completed -- waiting for pressure to equalize ... @18:19:13.64 Intake.select :close @18:19:13.82 SSV.dial :exhaust @18:19:14.71 Exhaust.select :open @18:19:14.85 SS.reconfigure SSexhale @18:19:14.92 SS.seek 0ml @18:19:24.56 Exhaust.select :close @18:19:24.70 SSV.dial CBV @18:19:24.73 CBV.dial SSV @18:19:25.65 Allowing 130s for 25.0ml of remaining 1450.0ml @18:19:25.68 Intake.select :open @18:19:27.89 Intake @15.9psia, vacuum from 9.4 to 13.4psi @18:19:27.91 SS.reconfigure SSinhale.with maxSpeed: 43 @18:19:28.00 SS.seek 25ml @18:19:33.91 Vacuum too high -- waiting up to 40s @18:19:40.17 Intake @16.1psia, vacuum from 9.6 to 13.6psi @18:19:40.20 SS.reconfigure SSinhale.with maxSpeed: 39 @18:19:40.29 SS.seek 25ml @18:19:46.28 Vacuum too high -- waiting up to 40s @18:19:52.87 SS.reconfigure SSinhale.with maxSpeed: 35 @18:19:52.94 SS.seek 25ml @18:20:01.94 Vacuum too high -- waiting up to 40s @18:20:10.79 Intake @16.3psia, vacuum from 9.8 to 13.8psi @18:20:10.82 SS.reconfigure SSinhale.with maxSpeed: 32 @18:20:10.91 SS.seek 25ml @18:20:20.66 Vacuum too high -- waiting up to 40s @18:20:29.22 Intake @16.2psia, vacuum from 9.7 to 13.7psi @18:20:29.25 SS.reconfigure SSinhale.with maxSpeed: 29 @18:20:29.34 SS.seek 25ml @18:20:41.22 Vacuum too high -- waiting up to 40s @18:20:51.11 Intake @16.1psia, vacuum from 9.6 to 13.6psi @18:20:51.13 SS.reconfigure SSinhale.with maxSpeed: 26 @18:20:51.22 SS.seek 25ml @18:21:07.20 Stroke completed -- waiting for pressure to equalize ... @18:21:08.32 Ignoring excess vacuum after stroke's end ... @18:21:14.87 delay 5 seconds @18:21:19.88 ATV.dial :air2 @18:21:21.11 AS.seek 0.52ml @18:21:25.71 Intake.select :close @18:21:25.89 SSV.dial :exhaust @18:21:26.80 Exhaust.select :open @18:21:26.94 SS.reconfigure SSexhale @18:21:27.01 SS.seek 0ml @18:21:36.62 Exhaust.select :close @18:21:36.76 SSV.dial CBV @18:21:36.79 CBV.dial SSV @18:21:37.71 Allowing 130s for 25.0ml of remaining 1425.0ml @18:21:37.73 Intake.select :open @18:21:39.93 Intake @15.8psia, vacuum from 9.3 to 13.3psi @18:21:39.96 SS.reconfigure SSinhale.with maxSpeed: 26 @18:21:40.05 SS.seek 25ml @18:21:52.32 Vacuum too high -- waiting up to 40s @18:21:59.29 Intake @15.8psia, vacuum from 9.3 to 13.3psi @18:21:59.32 SS.reconfigure SSinhale.with maxSpeed: 23 @18:21:59.41 SS.seek 25ml @18:22:16.88 Vacuum too high -- waiting up to 40s @18:22:27.86 Intake @16.0psia, vacuum from 9.5 to 13.5psi @18:22:27.88 SS.reconfigure SSinhale.with maxSpeed: 21 @18:22:27.97 SS.seek 25ml @18:22:56.08 Vacuum too high -- waiting up to 40s @18:23:01.96 delay 5 seconds @18:23:07.05 ATV.dial ASV @18:23:08.08 ASV.dial :wasteR @18:23:08.51 AS.seek 0.3ml @18:23:10.34 SS.reconfigure SSinhale.with maxSpeed: 19 @18:23:10.41 SS.seek 25ml @18:23:36.42 Stroke completed -- waiting for pressure to equalize ... @18:23:47.74 SS.reconfigure SSconfig @18:23:47.81 SS.hold @18:23:47.97 Intake.select :close @18:23:48.15 ShallowSampler::Clogged in WCR -- progress too slow after 100.0ml @18:23:48.17 SSV.dial :exhaust @18:23:49.07 Exhaust.select :open @18:23:49.21 SS.reconfigure SSexhale @18:23:49.29 SS.seek 0ml @18:23:53.10 delay 20 seconds @18:23:58.96 Exhaust.select :close @18:23:59.10 Sampled 100.0ml @18:23:59.12 Drying puck before fixing for 4:00 @18:23:59.14 CBV.dial :waste2b @18:24:00.07 delay 1:00 @18:24:13.14 AS.seek 0.07ml @18:24:59.68 delay 20 seconds @18:25:00.10 CSV.dial :air @18:25:00.65 CS.reconfigure CSfast @18:25:00.73 CS.seek 10ml @18:25:19.71 ATV.dial :waste2 @18:25:20.49 AS.reconfigure ASconfig @18:25:20.56 AS.seek :empty @18:25:24.97 Elution @18:25:24.98 ATV.dial :air1 @18:25:26.17 AS.reconfigure ASslow1 @18:25:26.24 AS.seek 0.049ml @18:25:27.78 Pumping wcr puck from tube 2 up to 55psi... @18:25:27.80 CBV.dial SSV @18:25:29.30 CBV.dial between CSV and waste2b @18:25:30.49 CSV.dial between air and CTV @18:25:30.89 CS.seek 7.326ml @18:25:36.59 delay 3 seconds @18:25:38.93 CTV.dial CSV,avoiding: :air @18:25:38.97 CSV.dial CTV @18:25:39.60 ATV.dial :ElutionWater @18:25:39.94 CS.reconfigure CSpressurize @18:25:40.01 CS.seek 0ml @18:25:40.33 AS.seek 0.109ml @18:25:52.68 delay 3 seconds @18:25:55.71 ATV.dial ASV,via: :air2 @18:25:57.88 ASV.dial :SPEelution @18:25:58.49 AFV.select :carrierSolution @18:25:58.66 AS.reconfigure ASnorm @18:25:58.73 AS.seek 0.759ml @18:25:59.90 CSV.dial :air @18:26:00.99 CS.reconfigure CSfast @18:26:01.09 CS.seek 10ml @18:26:28.22 CSV.dial between air and CTV @18:26:28.63 CS.seek 2.02ml @18:26:31.77 delay 3 seconds @18:26:34.79 AFV.select :holdingCoil @18:26:34.98 AS.reconfigure ASslow1 @18:26:35.05 AS.seek 0.474ml @18:26:50.71 CTV.dial CSV,avoiding: :air @18:26:50.74 CSV.dial CTV @18:26:51.14 CS.reconfigure CSpressurize @18:26:51.21 CS.seek 0ml @18:26:52.08 CBV.dial :waste2b @18:26:52.49 CS.reconfigure CSconfig @18:26:52.57 CS.seek :empty @18:26:58.59 delay 1:00 @18:27:32.67 delay 5 seconds @18:27:37.77 AS.reconfigure ASslow2 @18:27:37.84 AS.seek 0.439ml @18:27:58.62 CTV.dial :air,avoiding: :intake @18:28:00.26 Fix #1 of 2 (with rnal) for 2:00 @18:28:00.27 CSV.dial CSR @18:28:00.89 Pulling 2ml of CSR.rnal @18:28:00.90 CSR.select :rnal @18:28:01.06 CS.seek 2ml @18:28:07.28 delay 5 seconds @18:28:12.30 CSR.select :air @18:28:12.45 CS.reconfigure CSfast @18:28:12.53 CS.seek 10ml @18:28:13.45 delay 1:00 @18:28:34.37 Pumping wcr puck from tube 2 up to 55psi... @18:28:34.39 CBV.dial SSV @18:28:35.88 CBV.dial between CSV and waste2b @18:28:37.05 CSV.dial between air and CTV @18:28:37.93 CS.seek 7.234ml @18:28:46.06 CTV.dial CSV,avoiding: :air @18:28:46.09 CSV.dial CTV @18:28:47.76 CS.reconfigure CSpressurize @18:28:47.84 CS.seek 0ml @18:29:07.43 CSV.dial :air @18:29:08.00 CS.reconfigure CSfast @18:29:08.08 CS.seek 10ml @18:29:13.48 pushing eluate to holding coil @18:29:13.57 AS.seek 0.279ml @18:29:35.10 CSV.dial between air and CTV @18:29:35.51 CS.seek 2.174ml @18:29:57.00 CTV.dial CSV,avoiding: :air @18:29:57.03 CSV.dial CTV @18:29:57.43 CS.reconfigure CSpressurize @18:29:57.50 CS.seek 0ml @18:29:59.42 delay 2:00 @18:31:54.70 delay 30 seconds @18:31:59.45 CBV.dial :waste2b @18:31:59.85 CS.reconfigure CSconfig @18:31:59.93 CS.seek :empty @18:32:05.62 delay 1:00 @18:32:24.73 ATV.dial :waste,via: :air3 @18:32:26.51 AS.reconfigure ASconfig @18:32:26.58 AS.seek :empty @18:32:42.99 Parking Bleach in LysateSource line and SPEcolumn @18:32:43.00 ATV.dial :air3 @18:32:44.41 AS.reconfigure ASslow1 @18:32:44.49 AS.seek 0.029ml @18:32:50.89 ATV.dial :ElutionWater @18:32:51.53 AS.seek 0.059ml @18:32:57.85 ATV.dial :air3 @18:32:58.49 AS.seek 0.069ml @18:33:00.79 delay 2 seconds @18:33:02.82 ATV.dial :bleach @18:33:03.33 AS.seek 0.769ml @18:33:05.65 CTV.dial :air,avoiding: :intake @18:33:07.26 Fix #2 of 2 (with rnal) for 2:00 @18:33:07.28 CSV.dial CSR @18:33:07.90 Pulling 2ml of CSR.rnal @18:33:07.92 CSR.select :rnal @18:33:08.08 CS.seek 2ml @18:33:14.22 delay 5 seconds @18:33:19.24 CSR.select :air @18:33:19.40 CS.reconfigure CSfast @18:33:19.48 CS.seek 10ml @18:33:41.22 Pumping wcr puck from tube 2 up to 55psi... @18:33:41.24 CBV.dial SSV @18:33:42.73 CBV.dial between CSV and waste2b @18:33:43.90 CSV.dial between air and CTV @18:33:44.65 CS.seek 7.221ml @18:33:52.91 CTV.dial CSV,avoiding: :air @18:33:52.94 CSV.dial CTV @18:33:54.61 CS.reconfigure CSpressurize @18:33:54.70 CS.seek 0ml @18:34:14.25 CSV.dial :air @18:34:14.81 CS.reconfigure CSfast @18:34:14.89 CS.seek 10ml @18:34:41.97 CSV.dial between air and CTV @18:34:42.38 CS.seek 2.253ml @18:35:03.66 CTV.dial CSV,avoiding: :air @18:35:03.69 CSV.dial CTV @18:35:04.08 CS.reconfigure CSpressurize @18:35:04.16 CS.seek 0ml @18:35:06.61 delay 2:00 @18:35:24.38 delay 3 seconds @18:35:27.40 ATV.dial :air3 @18:35:27.99 delay 3 seconds @18:35:31.08 AS.seek 0.794ml @18:35:36.44 delay 3 seconds @18:35:39.46 ATV.dial :bleach @18:35:40.13 AS.seek 0.779ml @18:35:43.43 delay 3 seconds @18:35:46.46 ATV.dial :AIV @18:35:47.77 AS.reconfigure ASconfig @18:35:47.84 AS.seek 0.015ml @18:36:32.08 ATV.dial :waste @18:36:33.65 AS.seek :empty @18:36:34.87 ATV.dial :air3 @18:36:35.59 AS.reconfigure ASslow1 @18:36:35.66 AS.seek 0.009ml @18:36:37.99 delay 2 seconds @18:36:40.02 ATV.dial :bleach @18:36:40.50 AS.seek 0.579ml @18:37:06.64 CBV.dial :waste2b @18:37:07.05 CS.reconfigure CSconfig @18:37:07.13 CS.seek :empty @18:37:12.41 delay 1:00 @18:38:12.44 CTV.dial :air,avoiding: :intake @18:38:14.67 Storing wcr puck in Collection Clamp to tube 1 @18:38:14.90 SC.reconfigure SCtight @18:38:14.98 SC.seek 1 @18:38:17.15 SC.reconfigure SCloose @18:38:17.41 Elbow.reconfigure TightElbow @18:38:17.50 Elbow.seek 1 @18:38:19.42 Forearm.reconfigure TightForearm @18:38:19.50 Forearm.seek 1 + 700 counts @18:38:20.55 SE.reconfigure SEpick @18:38:20.66 SE.seek beamBreak @18:38:23.60 SE.configure SEconfig @18:38:23.79 Elbow.reconfigure LimpElbow @18:38:23.87 Forearm.reconfigure LooseForearm @18:38:23.94 Forearm.seek 1 @18:38:24.31 Elbow.hold @18:38:24.40 Elbow.reconfigure TightElbow @18:38:24.53 SE.seek 4.08 Pucks below Top Plate @18:38:25.08 Forearm.force -20 @18:38:25.15 Forearm.seek 1 + 313 counts @18:38:25.53 Forearm.reconfigure TightForearm @18:38:25.61 Forearm.seek 1 + 700 counts @18:38:26.05 SE.seek 5.08 Pucks below Top Plate @18:38:26.83 Forearm.reconfigure LooseForearm @18:38:26.90 Forearm.seek :retract @18:38:27.92 Elbow.seek CC @18:38:29.63 Elbow.reconfigure LooseElbow @18:38:29.76 Forearm.seek CC @18:38:30.82 Hand.close @18:38:31.57 CC.seek :open @18:38:35.43 delay 3 seconds @18:38:36.14 Forearm.seek :retract @18:38:37.21 Elbow.reconfigure TightElbow @18:38:37.28 Elbow.seek 1 @18:38:38.45 ATV.dial :air3 @18:38:39.04 delay 3 seconds @18:38:39.25 Elbow.reconfigure LimpElbow @18:38:39.33 Forearm.seek 1 @18:38:40.39 Elbow.hold @18:38:40.50 Elbow.reconfigure TightElbow @18:38:40.57 Hand.open @18:38:41.45 Forearm.force -20 @18:38:41.52 Forearm.seek 1 + 349 counts @18:38:42.45 Forearm.reconfigure TightForearm @18:38:42.52 Forearm.seek 1 + 700 counts @18:38:42.67 AS.seek 0.604ml @18:38:43.01 SE.seek :down @18:38:44.17 Forearm.reconfigure LooseForearm @18:38:44.25 Forearm.seek :retract @18:38:45.41 SE.coast @18:38:45.48 SC.coast @18:38:45.55 Starting finalPurge @18:38:45.57 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @18:38:45.68 Hand.open @18:38:46.09 Elbow.seek FlushPuck::Garage @18:38:47.96 delay 3 seconds @18:38:48.14
Elbow.reconfigure LooseElbow @18:38:48.27 Forearm.seek :garage @18:38:49.31 Hand.close @18:38:50.21 Forearm.seek :retract @18:38:50.97 ATV.dial :bleach @18:38:51.29
Elbow.reconfigure TightElbow @18:38:51.36 Elbow.seek CC @18:38:51.64 AS.seek 0.589ml @18:38:54.32
Elbow.reconfigure LooseElbow @18:38:54.44 Forearm.reconfigure TightForearm @18:38:54.52 Forearm.seek CC @18:38:54.96 delay 3 seconds @18:38:55.90
CC.seek :closed @18:38:57.98 ATV.dial :ASV @18:38:59.19 ASV.dial :wasteR @18:38:59.85 AS.seek 0ml @18:39:00.16
Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.51 @18:39:00.27 Hand.open @18:39:01.14 Forearm.reconfigure LooseForearm @18:39:01.21 Forearm.seek :retract @18:39:02.31 Flushing the Collection Valve::Manifold @18:39:02.47 CSV.dial CSR @18:39:03.06 Pulling 5ml of CSR.flush @18:39:03.07 CSR.select :flush @18:39:03.23 CS.seek 5ml @18:39:17.38 delay 5 seconds @18:39:22.41 CSR.select :air @18:39:22.59 CS.reconfigure CSfast @18:39:22.67 CS.seek 7.5ml @18:39:30.16 CSV.dial :waste @18:39:31.77 CS.reconfigure CSconfig @18:39:31.85 CS.seek 0ml @18:39:52.25 Flushing lower manifold's waste1 port @18:39:52.41 CSV.dial :flush @18:39:52.97 CS.reconfigure CSfast @18:39:53.05 CS.seek 5ml @18:40:07.10 delay 5 seconds @18:40:12.13 CSV.dial :air @18:40:13.43 CS.seek 7ml @18:40:19.60 CSV.dial CTV @18:40:19.63
CTV.dial CSV @18:40:21.32 CBV.dial :waste1 @18:40:22.26 CS.seek 0ml @18:40:41.28 Flushing lower manifold's waste2b port @18:40:41.44 CSV.dial :flush @18:40:42.37 CS.seek 5ml @18:40:56.38 delay 5 seconds @18:40:58.65 delay 1:00 @18:41:01.40
CSV.dial :air @18:41:02.04 CS.seek 7ml @18:41:08.28 CSV.dial CTV @18:41:08.31
CTV.dial CSV @18:41:08.86 CBV.dial :waste2b @18:41:09.81 CS.seek 0ml @18:41:28.88 Flushing lower manifold's waste1 port @18:41:28.90 CSV.dial CSR @18:41:29.50 Pulling 4ml of CSR.mfbkill @18:41:29.51 CSR.select :mfbkill @18:41:29.68 CS.reconfigure CSconfig @18:41:29.75 CS.seek 4ml @18:41:41.13 delay 5 seconds @18:41:46.15 CSR.select :air @18:41:46.30 CS.reconfigure CSfast @18:41:46.38 CS.seek 10ml @18:41:58.67 AFV.select :carrierSolution @18:41:58.80 delay 2 seconds @18:42:00.83 AS.reconfigure ASconfig @18:42:00.89 AS.seek 1ml @18:42:02.94 CSV.dial CTV @18:42:02.97
CTV.dial CSV @18:42:03.55 CBV.dial :waste1 @18:42:04.48 CS.seek 0ml @18:42:31.50 Flushing lower manifold's waste2b port @18:42:31.52 CSV.dial CSR @18:42:32.16 Pulling 4ml of CSR.mfbkill @18:42:32.17 CSR.select :mfbkill @18:42:32.34 CS.reconfigure CSconfig @18:42:32.40 CS.seek 4ml @18:42:43.94 delay 5 seconds @18:42:48.97 CSR.select :air @18:42:49.11 CS.reconfigure CSfast @18:42:49.19 CS.seek 10ml @18:42:58.73 delay 1 second @18:42:59.74 AFV.select :holdingCoil @18:42:59.87 delay 1 second @18:43:00.90 ATV.dial ASV @18:43:00.91 ASV.dial :wasteR @18:43:00.94 AS.reconfigure ASslow1 @18:43:01.01 AS.seek 0ml @18:43:05.82 CSV.dial CTV @18:43:05.85
CTV.dial CSV @18:43:06.43 CBV.dial :waste2b @18:43:07.36 CS.seek 0ml @18:43:34.22 Killing upper manifold @18:43:34.23 CSV.dial CSR @18:43:34.86 Pulling 4ml of CSR.mfbkill @18:43:34.87 CSR.select :mfbkill @18:43:35.03 CS.reconfigure CSconfig @18:43:35.09 CS.seek 4ml @18:43:46.54 delay 5 seconds @18:43:51.56 CSR.select :air @18:43:51.71 CS.reconfigure CSfast @18:43:51.78 CS.seek 10ml @18:44:08.22 CSV.dial CBV @18:44:08.25
CBV.dial CSV @18:44:09.57 CTV.dial :air @18:44:11.17 CS.seek 0ml @18:44:38.10 delay 7 seconds @18:44:45.14 CS.seek 3ml @18:44:53.87 CSV.dial :waste @18:44:54.42 CS.seek 0ml @18:45:03.16 Flushing upper manifold @18:45:03.32 CSV.dial :flush @18:45:03.87 CS.seek 5ml @18:45:17.82 delay 5 seconds @18:45:22.85 CSV.dial :air @18:45:24.02 CS.seek 7ml @18:45:30.26 CSV.dial CBV @18:45:30.29
CBV.dial CSV @18:45:31.57 CTV.dial :air @18:45:31.60 CS.seek 0ml @18:45:50.72 delay 7 seconds @18:45:57.76 CS.seek 3ml @18:46:06.54 CSV.dial :waste @18:46:07.11 CS.seek 0ml @18:46:15.92 CSV.dial :flush @18:46:16.49 CS.seek 3ml @18:46:22.42 delay 20 seconds @18:46:25.29
delay 5 seconds @18:46:30.32 CSV.dial :air @18:46:30.94 CS.seek 5ml @18:46:37.10 CSV.dial :air @18:46:37.12 CS.seek 0ml @18:46:42.45 ATV.dial :air3 @18:46:44.05 AS.seek 0.59ml @18:46:51.29
delay 7 seconds @18:46:58.33 CS.seek 3ml @18:47:07.07 CSV.dial :waste @18:47:08.00 CS.seek 0ml @18:47:16.82 Flushing lower manifold's waste1 port @18:47:16.98 CSV.dial :flush @18:47:17.55 CS.seek 5ml @18:47:31.51 delay 5 seconds @18:47:36.54 CSV.dial :air @18:47:37.15 CS.seek 7ml @18:47:43.38 CSV.dial CTV @18:47:43.41
CTV.dial CSV @18:47:45.10 CBV.dial :waste1 @18:47:45.66 CS.seek 0ml @18:48:04.73 Flushing lower manifold's waste2b port @18:48:04.89 CSV.dial :flush @18:48:05.83 CS.seek 5ml @18:48:19.66 delay 5 seconds @18:48:24.69 CSV.dial :air @18:48:25.33 CS.seek 7ml @18:48:31.48 CSV.dial CTV @18:48:31.52
CTV.dial CSV @18:48:32.05 CBV.dial :waste2b @18:48:33.00 CS.seek 0ml @18:48:43.10 delay 3 seconds @18:48:46.12 ATV.dial :ASV @18:48:47.60 ASV.dial :wasteR @18:48:47.70 AS.seek 0.09ml @18:48:52.01
Garaging FlushPuck in Collection Clamp @18:48:52.11 Elbow.reconfigure TightElbow @18:48:52.18 Elbow.seek CC @18:48:52.47 Elbow.reconfigure LooseElbow @18:48:52.60 Forearm.seek CC @18:48:53.69 Hand.close @18:48:54.46 CC.seek :open @18:48:58.89 Forearm.seek :retract @18:48:59.98 Elbow.reconfigure TightElbow @18:49:00.05 Elbow.seek :garage @18:49:03.25 Elbow.reconfigure LooseElbow @18:49:03.38 Forearm.reconfigure TightForearm @18:49:03.45 Forearm.seek :garage @18:49:04.62 Hand.open @18:49:05.53 Forearm.reconfigure LooseForearm @18:49:05.60 Forearm.seek :retract @18:49:07.23 Completed [mbaPhase] begun 17:04:22 @18:49:07.25 Extending 20invasivedeploymfbtest until completion of MFBPCR @18:50:28.56 delay 30 seconds @18:50:58.59 ATV.dial :extSrc @18:50:59.49 delay 3 seconds @18:51:02.52 ATV.dial :ElutionWater @18:51:03.70 AS.seek 0.12ml @18:51:10.06 ATV.dial :air3 @18:51:10.72 AS.seek 0.13ml @18:51:13.04 delay 2 seconds @18:51:15.07 ATV.dial :bleach @18:51:15.56 AS.seek 0.23ml @18:51:35.98 delay 3 seconds @18:51:39.01 ATV.dial :air3 @18:51:39.59 delay 3 seconds @18:51:42.68 AS.seek 0.255ml @18:51:48.03 delay 3 seconds @18:51:51.06 ATV.dial :bleach @18:51:51.72 AS.seek 0.24ml @18:51:55.09 delay 3 seconds @18:51:58.12 ATV.dial :ASV @18:51:59.18 ASV.dial :SPEdelivery @18:51:59.59 AFV.select :carrierSolution @18:51:59.72 delay 2 seconds @18:52:01.75 AS.reconfigure ASconfig @18:52:01.82 AS.seek 0.5ml @18:52:17.09 delay 1 second @18:52:18.10 AFV.select :holdingCoil @18:52:18.27 AS.reconfigure ASslow1 @18:52:18.34 AS.seek 0.17ml @18:53:25.02 SPE.hold 2:46:40,95C @18:53:25.07 delay 3:00 @18:56:25.10 AFV.select :carrierSolution @18:56:25.23 delay 2 seconds @18:56:27.26 AS.reconfigure ASconfig @18:56:27.32 AS.seek 1ml @18:57:15.34 delay 1 second @18:57:16.35 AFV.select :holdingCoil @18:57:16.48 delay 1 second @18:57:17.50 ASV.dial :wasteD @18:57:18.72 AS.seek 0ml @18:58:16.52 Priming Eluate @18:58:16.53 AFV.select :carrierSolution @18:58:16.67 delay 2 seconds @18:58:18.70 AS.seek 0.5ml @18:58:47.73 delay 1 second @18:58:48.74 AFV.select :holdingCoil @18:58:48.86 ATV.dial ASV @18:58:48.87 ASV.dial :SPErecovery @18:58:49.52 Swish #1 of 2 @18:58:49.62 AS.reconfigure ASslow0 @18:58:49.69 AS.seek 0.6ml @18:59:00.16 AS.seek 0.5ml @18:59:10.58 Swish #2 of 2 @18:59:10.69 AS.seek 0.6ml @18:59:21.19 AS.seek 0.5ml @18:59:31.59 delay 3 seconds @18:59:34.67 AS.reconfigure ASslow1 @18:59:34.75 AS.seek 0.684ml @19:00:12.09 delay 3 seconds @19:00:15.12 Eluate primed @19:00:15.13 ATV.dial :waste2 @19:00:15.81 AS.reconfigure ASnorm @19:00:15.91 AS.seek 0ml @19:00:50.78 SPE.coast @19:00:50.88 ASV.dial :SPErecovery @19:00:50.91 Setting up PCR for OM -- writing /var/log/esp/OM20may0319h225ml.pcr @19:00:50.95 Building PCR Slug from ASV with MM3 mix, PP5 primer @19:00:50.97 Priming with carrier @19:00:50.99 ATV.dial ARV @19:00:52.02 ARV.dial :carrier @19:00:52.39 AS.seek 0.5ml @19:01:17.92 AFV.select :carrierSolution @19:01:18.05 delay 2 seconds @19:01:20.08 AS.reconfigure ASconfig @19:01:20.15 AS.seek 1ml @19:01:49.18 delay 1 second @19:01:50.20 AFV.select :holdingCoil @19:01:50.33 delay 1 second @19:01:51.36 ATV.dial :waste @19:01:52.88 AS.reconfigure ASnorm @19:01:52.96 AS.seek :empty @19:02:43.70 AFV.select :carrierSolution @19:02:43.84 delay 2 seconds @19:02:45.87 AS.reconfigure ASconfig @19:02:45.94 AS.seek 0.5ml @19:03:15.04 delay 1 second @19:03:16.05 AFV.select :holdingCoil @19:03:16.18 Building Slug @19:03:16.19 ATV.dial :ARV @19:03:17.96 delay 2 seconds @19:03:19.99 ARV.dial :air1 @19:03:20.42 delay 2 seconds @19:03:22.51 AS.reconfigure ASslow2 @19:03:22.59 AS.seek 0.506ml @19:03:28.81 delay 2 seconds @19:03:30.84 ARV.dial :MM3 @19:03:31.92 delay 2 seconds @19:03:34.52 Adding MM3 @19:03:34.63 AS.seek 0.524ml @19:03:53.07 delay 25 seconds @19:04:18.10 ARV.dial :PP5 @19:04:18.52 Adding PP5 @19:04:18.54 delay 2 seconds @19:04:20.63 AS.seek 0.53ml @19:04:26.88 delay 10 seconds @19:04:36.91 ARV.dial :air3 @19:04:37.31 adding ARV air @19:04:37.33 delay 2 seconds @19:04:39.42 AS.seek 0.536ml @19:04:45.82 delay 5 seconds @19:04:50.85 ARV.dial :carrier @19:04:52.12 delay 7 seconds @19:04:59.21 AS.seek 0.594ml @19:05:57.87 delay 5 seconds @19:06:02.90 ATV.dial :ASV @19:06:03.32 delay 2 seconds @19:06:05.41 AS.seek 0.6ml @19:06:11.73 delay 4 seconds @19:06:15.76 ATV.dial ARV @19:06:16.34 delay 2 seconds @19:06:18.37 ARV.dial :carrier @19:06:18.38 delay 2 seconds @19:06:20.49 AS.seek 0.65ml @19:07:11.04 delay 4 seconds @19:07:15.07 ATV.dial PCR @19:07:15.71 ASV.dial :waste1 @19:07:16.64 ARV.dial :air1 @19:07:17.06 Positioning Slug by 0.324ml @19:07:17.17 AS.seek 0.326ml @19:12:43.32 delay 5 seconds @19:12:48.35 ATV.dial ARV @19:12:49.15 ASV.dial :PCRlock @19:12:49.56 delay 5 seconds @19:12:54.59 Running OM Profile60C350 thermocycling @19:12:54.61 PCR.program Profile60C350 @19:12:54.74 Profile60C350 should complete in < 2:02:45 @19:12:54.75 PCR.run 2:38:26.25 @20:49:56.52 PCR.off @20:49:58.24 PCR for OM completed -- closed /var/log/esp/OM20may0319h225ml.pcr @20:49:58.33 Cleaning PCR @20:49:58.34 AFV.select :carrierSolution @20:49:58.48 delay 2 seconds @20:50:00.51 AS.reconfigure ASconfig @20:50:00.58 AS.seek 0.5ml @20:50:10.92 delay 1 second @20:50:11.94 AFV.select :holdingCoil @20:50:12.07 ASV.dial :waste1 @20:50:12.46 ATV.dial :air3 @20:50:13.80 AS.reconfigure ASslow1 @20:50:13.87 AS.seek 0.51ml @20:50:16.18 delay 2 seconds @20:50:18.20 ATV.dial :bleach @20:50:18.85 AS.seek 0.81ml @20:51:19.46 delay 3 seconds @20:51:22.49 ATV.dial :air3 @20:51:23.07 delay 3 seconds @20:51:26.16 AS.seek 0.835ml @20:51:31.56 delay 3 seconds @20:51:34.59 ATV.dial :bleach @20:51:35.24 AS.seek 0.82ml @20:51:38.51 delay 3 seconds @20:51:41.54 ARV.dial :waste @20:51:42.17 delay 3 seconds @20:51:45.20 ATV.dial :ARV @20:51:46.13 AS.reconfigure ASconfig @20:51:46.20 AS.seek 0.47ml @20:52:06.64 AFV.select :carrierSolution @20:52:06.78 delay 2 seconds @20:52:08.81 AS.seek 1ml @20:52:39.56 delay 1 second @20:52:40.57 AFV.select :holdingCoil @20:52:40.69 delay 1 second @20:52:41.72 AS.reconfigure ASfast @20:52:41.79 AS.seek 0ml @20:53:09.16 AFV.select :carrierSolution @20:53:09.30 delay 2 seconds @20:53:11.33 AS.reconfigure ASconfig @20:53:11.40 AS.seek 1ml @20:54:09.17 delay 1 second @20:54:10.18 AFV.select :holdingCoil @20:54:10.31 delay 1 second @20:54:11.34 AS.reconfigure ASfast @20:54:11.41 AS.seek 0ml @20:54:38.77 AFV.select :carrierSolution @20:54:38.91 delay 2 seconds @20:54:40.94 AS.reconfigure ASconfig @20:54:41.01 AS.seek 1ml @20:55:38.84 delay 1 second @20:55:39.85 AFV.select :holdingCoil @20:55:39.98 delay 1 second @20:55:41.01 AS.reconfigure ASfast @20:55:41.08 AS.seek 0ml @20:56:08.46 ARV.dial between carrier and air1 @20:56:08.98 ATV.dial :air3 @20:56:10.31 AS.reconfigure ASslow1 @20:56:10.38 AS.seek 0.01ml @20:56:12.70 delay 2 seconds @20:56:14.72 ATV.dial :bleach @20:56:15.38 AS.seek 0.81ml @20:58:56.62 delay 3 seconds @20:58:59.65 ATV.dial :air3 @20:59:00.23 delay 3 seconds @20:59:03.32 AS.seek 0.835ml @20:59:08.79 delay 3 seconds @20:59:11.82 ATV.dial :bleach @20:59:12.47 AS.seek 0.82ml @20:59:15.79 delay 3 seconds @20:59:18.82 ATV.dial PCR @20:59:19.23 AS.reconfigure ASconfig @20:59:19.30 AS.seek :empty @21:00:06.79 PCR.program :bleachProfile @21:00:06.86 PCR program should complete in < 2:45 @21:00:06.87 PCR.run 10:00 @21:03:02.65 PCR.off @21:03:02.69 AFV.select :carrierSolution @21:03:02.82 delay 2 seconds @21:03:04.85 AS.seek 1ml @21:04:02.71 delay 1 second @21:04:03.72 AFV.select :holdingCoil @21:04:03.85 delay 1 second @21:04:05.40 AS.reconfigure ASfast @21:04:05.54 AS.seek 0ml @21:04:32.92 delay 3 seconds @21:04:35.94 AFV.select :carrierSolution @21:04:36.07 delay 2 seconds @21:04:38.10 AS.reconfigure ASconfig @21:04:38.17 AS.seek 1ml @21:05:35.93 delay 1 second @21:05:36.94 AFV.select :holdingCoil @21:05:37.07 delay 1 second @21:05:38.10 AS.reconfigure ASfast @21:05:38.17 AS.seek 0ml @21:06:05.54 delay 3 seconds @21:06:08.56 AFV.select :carrierSolution @21:06:08.69 delay 2 seconds @21:06:10.72 AS.reconfigure ASconfig @21:06:10.79 AS.seek 1ml @21:07:08.65 delay 1 second @21:07:09.66 AFV.select :holdingCoil @21:07:09.79 delay 1 second @21:07:10.82 AS.reconfigure ASfast @21:07:10.89 AS.seek 0ml @21:07:38.26 delay 3 seconds @21:07:41.29 AFV.select :carrierSolution @21:07:41.41 delay 2 seconds @21:07:43.44 AS.reconfigure ASconfig @21:07:43.52 AS.seek 1ml @21:08:41.34 delay 1 second @21:08:42.35 AFV.select :holdingCoil @21:08:42.48 delay 1 second @21:08:43.51 AS.reconfigure ASfast @21:08:43.58 AS.seek 0ml @21:09:10.95 delay 3 seconds @21:09:13.97 AFV.select :carrierSolution @21:09:14.10 delay 2 seconds @21:09:16.13 AS.reconfigure ASconfig @21:09:16.20 AS.seek 1ml @21:10:14.03 delay 1 second @21:10:15.04 AFV.select :holdingCoil @21:10:15.17 delay 1 second @21:10:16.20 AS.reconfigure ASnorm @21:10:16.27 AS.seek 0ml @21:11:07.03 Setting up PCR for CG -- writing /var/log/esp/CG20may0321h225ml.pcr @21:11:07.05 Building PCR Slug from ASV with MM1 mix, PP2 primer @21:11:07.09 Priming with carrier @21:11:07.11 ATV.dial ARV @21:11:07.76 ARV.dial :carrier @21:11:08.13 AS.seek 0.5ml @21:11:33.69 AFV.select :carrierSolution @21:11:33.83 delay 2 seconds @21:11:35.86 AS.reconfigure ASconfig @21:11:35.93 AS.seek 1ml @21:12:04.96 delay 1 second @21:12:05.97 AFV.select :holdingCoil @21:12:06.10 delay 1 second @21:12:07.13 ATV.dial :waste @21:12:08.82 AS.reconfigure ASnorm @21:12:08.89 AS.seek :empty @21:12:59.66 AFV.select :carrierSolution @21:12:59.80 delay 2 seconds @21:13:01.83 AS.reconfigure ASconfig @21:13:01.90 AS.seek 0.5ml @21:13:31.04 delay 1 second @21:13:32.06 AFV.select :holdingCoil @21:13:32.19 Building Slug @21:13:32.20 ATV.dial :ARV @21:13:33.89 delay 2 seconds @21:13:35.92 ARV.dial :air1 @21:13:36.33 delay 2 seconds @21:13:38.42 AS.reconfigure ASslow2 @21:13:38.49 AS.seek 0.506ml @21:13:44.70 delay 2 seconds @21:13:46.73 ARV.dial :MM1 @21:13:47.34 delay 2 seconds @21:13:49.37 Adding MM1 @21:13:49.46 AS.seek 0.524ml @21:14:07.74 delay 25 seconds @21:14:32.77 ARV.dial :PP2 @21:14:33.18 Adding PP2 @21:14:33.20 delay 2 seconds @21:14:35.29 AS.seek 0.53ml @21:14:41.59 delay 10 seconds @21:14:51.63 ARV.dial :air2 @21:14:52.04 adding ARV air @21:14:52.05 delay 2 seconds @21:14:54.15 AS.seek 0.536ml @21:15:00.76 delay 5 seconds @21:15:05.79 ARV.dial :carrier @21:15:07.12 delay 7 seconds @21:15:14.21 AS.seek 0.594ml @21:16:12.79 delay 5 seconds @21:16:17.82 ATV.dial :ASV @21:16:18.25 delay 2 seconds @21:16:20.34 AS.seek 0.6ml @21:16:26.73 delay 4 seconds @21:16:30.76 ATV.dial ARV @21:16:31.34 delay 2 seconds @21:16:33.37 ARV.dial :carrier @21:16:33.38 delay 2 seconds @21:16:35.48 AS.seek 0.65ml @21:17:26.18 delay 4 seconds @21:17:30.21 ATV.dial PCR @21:17:30.84 ASV.dial :waste1 @21:17:30.85 ARV.dial :air1 @21:17:31.30 Positioning Slug by 0.324ml @21:17:32.00 AS.seek 0.326ml @21:22:58.29 delay 5 seconds @21:23:03.32 ATV.dial ARV @21:23:04.14 ASV.dial :PCRlock @21:23:04.56 delay 5 seconds @21:23:09.59 Running CG Profile60C300 thermocycling @21:23:09.60 PCR.program Profile60C300 @21:23:09.72 Profile60C300 should complete in < 1:59:25 @21:23:09.74 PCR.run 2:34:16.25 @22:59:21.88 PCR.off @22:59:22.57 PCR for CG completed -- closed /var/log/esp/CG20may0321h225ml.pcr @22:59:22.66 Cleaning PCR @22:59:22.67 AFV.select :carrierSolution @22:59:22.81 delay 2 seconds @22:59:24.84 AS.reconfigure ASconfig @22:59:24.91 AS.seek 0.5ml @22:59:35.26 delay 1 second @22:59:36.27 AFV.select :holdingCoil @22:59:36.40 ASV.dial :waste1 @22:59:36.79 ATV.dial :air3 @22:59:38.11 AS.reconfigure ASslow1 @22:59:38.18 AS.seek 0.51ml @22:59:40.48 delay 2 seconds @22:59:42.50 ATV.dial :bleach @22:59:43.16 AS.seek 0.81ml @23:00:43.78 delay 3 seconds @23:00:46.81 ATV.dial :air3 @23:00:47.39 delay 3 seconds @23:00:50.48 AS.seek 0.835ml @23:00:55.78 delay 3 seconds @23:00:58.80 ATV.dial :bleach @23:00:59.46 AS.seek 0.82ml @23:01:02.79 delay 3 seconds @23:01:05.82 ARV.dial :waste @23:01:06.45 delay 3 seconds @23:01:09.47 ATV.dial :ARV @23:01:10.39 AS.reconfigure ASconfig @23:01:10.47 AS.seek 0.47ml @23:01:30.89 AFV.select :carrierSolution @23:01:31.03 delay 2 seconds @23:01:33.06 AS.seek 1ml @23:02:03.89 delay 1 second @23:02:04.90 AFV.select :holdingCoil @23:02:05.03 delay 1 second @23:02:06.06 AS.reconfigure ASfast @23:02:06.13 AS.seek 0ml @23:02:33.50 AFV.select :carrierSolution @23:02:33.64 delay 2 seconds @23:02:35.67 AS.reconfigure ASconfig @23:02:35.74 AS.seek 1ml @23:03:33.58 delay 1 second @23:03:34.59 AFV.select :holdingCoil @23:03:34.72 delay 1 second @23:03:35.75 AS.reconfigure ASfast @23:03:35.82 AS.seek 0ml @23:04:03.19 AFV.select :carrierSolution @23:04:03.33 delay 2 seconds @23:04:05.36 AS.reconfigure ASconfig @23:04:05.43 AS.seek 1ml @23:05:03.20 delay 1 second @23:05:04.21 AFV.select :holdingCoil @23:05:04.34 delay 1 second @23:05:05.37 AS.reconfigure ASfast @23:05:05.44 AS.seek 0ml @23:05:32.81 ARV.dial between carrier and air1 @23:05:33.33 ATV.dial :air3 @23:05:35.27 AS.reconfigure ASslow1 @23:05:35.34 AS.seek 0.01ml @23:05:37.68 delay 2 seconds @23:05:39.70 ATV.dial :bleach @23:05:40.35 AS.seek 0.81ml @23:08:21.53 delay 3 seconds @23:08:24.56 ATV.dial :air3 @23:08:25.14 delay 3 seconds @23:08:28.23 AS.seek 0.835ml @23:08:33.57 delay 3 seconds @23:08:36.60 ATV.dial :bleach @23:08:37.26 AS.seek 0.82ml @23:08:40.58 delay 3 seconds @23:08:43.61 ATV.dial PCR @23:08:44.03 AS.reconfigure ASconfig @23:08:44.10 AS.seek :empty @23:09:31.58 PCR.program :bleachProfile @23:09:31.66 PCR program should complete in < 2:45 @23:09:31.67 PCR.run 10:00 @23:12:27.44 PCR.off @23:12:27.48 AFV.select :carrierSolution @23:12:27.60 delay 2 seconds @23:12:29.63 AS.seek 1ml @23:13:27.51 delay 1 second @23:13:28.52 AFV.select :holdingCoil @23:13:28.65 delay 1 second @23:13:29.68 AS.reconfigure ASfast @23:13:29.75 AS.seek 0ml @23:13:57.12 delay 3 seconds @23:14:00.14 AFV.select :carrierSolution @23:14:00.27 delay 2 seconds @23:14:02.30 AS.reconfigure ASconfig @23:14:02.37 AS.seek 1ml @23:15:00.15 delay 1 second @23:15:01.16 AFV.select :holdingCoil @23:15:01.29 delay 1 second @23:15:02.32 AS.reconfigure ASfast @23:15:02.39 AS.seek 0ml @23:15:29.76 delay 3 seconds @23:15:32.78 AFV.select :carrierSolution @23:15:32.91 delay 2 seconds @23:15:34.94 AS.reconfigure ASconfig @23:15:35.02 AS.seek 1ml @23:16:32.87 delay 1 second @23:16:33.88 AFV.select :holdingCoil @23:16:34.01 delay 1 second @23:16:35.04 AS.reconfigure ASfast @23:16:35.11 AS.seek 0ml @23:17:02.50 delay 3 seconds @23:17:05.53 AFV.select :carrierSolution @23:17:05.65 delay 2 seconds @23:17:07.68 AS.reconfigure ASconfig @23:17:07.76 AS.seek 1ml @23:18:05.53 delay 1 second @23:18:06.54 AFV.select :holdingCoil @23:18:06.67 delay 1 second @23:18:07.70 AS.reconfigure ASfast @23:18:07.77 AS.seek 0ml @23:18:35.11 delay 3 seconds @23:18:38.13 AFV.select :carrierSolution @23:18:38.26 delay 2 seconds @23:18:40.29 AS.reconfigure ASconfig @23:18:40.37 AS.seek 1ml @23:19:38.14 delay 1 second @23:19:39.15 AFV.select :holdingCoil @23:19:39.28 delay 1 second @23:19:40.31 AS.reconfigure ASnorm @23:19:40.38 AS.seek 0ml @23:20:31.13 Setting up PCR for ML -- writing /var/log/esp/ML20may0323h225ml.pcr @23:20:31.15 Building PCR Slug from ASV with MM2 mix, PP4 primer @23:20:31.17 Priming with carrier @23:20:31.20 ATV.dial ARV @23:20:31.84 ARV.dial :carrier @23:20:32.22 AS.seek 0.5ml @23:20:57.77 AFV.select :carrierSolution @23:20:57.91 delay 2 seconds @23:20:59.94 AS.reconfigure ASconfig @23:21:00.01 AS.seek 1ml @23:21:29.03 delay 1 second @23:21:30.04 AFV.select :holdingCoil @23:21:30.17 delay 1 second @23:21:31.20 ATV.dial :waste @23:21:32.89 AS.reconfigure ASnorm @23:21:32.97 AS.seek :empty @23:22:23.72 AFV.select :carrierSolution @23:22:23.86 delay 2 seconds @23:22:25.89 AS.reconfigure ASconfig @23:22:25.96 AS.seek 0.5ml @23:22:55.09 delay 1 second @23:22:56.11 AFV.select :holdingCoil @23:22:56.23 Building Slug @23:22:56.24 ATV.dial :ARV @23:22:57.92 delay 2 seconds @23:22:59.95 ARV.dial :air1 @23:23:00.37 delay 2 seconds @23:23:02.46 AS.reconfigure ASslow2 @23:23:02.53 AS.seek 0.506ml @23:23:09.31 delay 2 seconds @23:23:11.34 ARV.dial :MM2 @23:23:12.86 delay 2 seconds @23:23:14.89 Adding MM2 @23:23:14.99 AS.seek 0.524ml @23:23:33.70 delay 25 seconds @23:23:58.73 ARV.dial :PP4 @23:23:59.15 Adding PP4 @23:23:59.17 delay 2 seconds @23:24:01.26 AS.seek 0.53ml @23:24:08.00 delay 10 seconds @23:24:18.03 ARV.dial :air3 @23:24:18.45 adding ARV air @23:24:18.47 delay 2 seconds @23:24:20.56 AS.seek 0.536ml @23:24:26.76 delay 5 seconds @23:24:31.79 ARV.dial :carrier @23:24:33.08 delay 7 seconds @23:24:40.17 AS.seek 0.594ml @23:25:39.00 delay 5 seconds @23:25:44.03 ATV.dial :ASV @23:25:44.45 delay 2 seconds @23:25:46.54 AS.seek 0.6ml @23:25:52.83 delay 4 seconds @23:25:56.86 ATV.dial ARV @23:25:57.45 delay 2 seconds @23:25:59.48 ARV.dial :carrier @23:25:59.49 delay 2 seconds @23:26:01.59 AS.seek 0.65ml @23:26:52.33 delay 4 seconds @23:26:56.36 ATV.dial PCR @23:26:57.00 ASV.dial :waste1 @23:26:57.02 ARV.dial :air1 @23:26:57.46 Positioning Slug by 0.324ml @23:26:57.57 AS.seek 0.326ml @23:32:24.25 delay 5 seconds @23:32:29.28 ATV.dial ARV @23:32:30.09 ASV.dial :PCRlock @23:32:30.50 delay 5 seconds @23:32:35.53 Running ML Profile60C350 thermocycling @23:32:35.55 PCR.program Profile60C350 @23:32:35.68 Profile60C350 should complete in < 2:02:45 @23:32:35.69 PCR.run 2:38:26.25 @01:09:24.47CEST04-May-20 PCR.off @01:09:25.75 PCR for ML completed -- closed /var/log/esp/ML20may0323h225ml.pcr @01:09:25.83 Cleaning PCR @01:09:25.85 AFV.select :carrierSolution @01:09:25.98 delay 2 seconds @01:09:28.01 AS.reconfigure ASconfig @01:09:28.08 AS.seek 0.5ml @01:09:38.39 delay 1 second @01:09:39.40 AFV.select :holdingCoil @01:09:40.14 ASV.dial :waste1 @01:09:40.53 ATV.dial :air3 @01:09:41.87 AS.reconfigure ASslow1 @01:09:41.94 AS.seek 0.51ml @01:09:44.22 delay 2 seconds @01:09:46.24 ATV.dial :bleach @01:09:46.88 AS.seek 0.81ml @01:10:47.48 delay 3 seconds @01:10:50.51 ATV.dial :air3 @01:10:51.09 delay 3 seconds @01:10:54.18 AS.seek 0.835ml @01:10:59.59 delay 3 seconds @01:11:02.62 ATV.dial :bleach @01:11:03.27 AS.seek 0.82ml @01:11:06.58 delay 3 seconds @01:11:09.61 ARV.dial :waste @01:11:10.23 delay 3 seconds @01:11:13.26 ATV.dial :ARV @01:11:14.18 AS.reconfigure ASconfig @01:11:14.25 AS.seek 0.47ml @01:11:34.68 AFV.select :carrierSolution @01:11:34.82 delay 2 seconds @01:11:36.85 AS.seek 1ml @01:12:07.61 delay 1 second @01:12:08.62 AFV.select :holdingCoil @01:12:08.78 delay 1 second @01:12:09.81 AS.reconfigure ASfast @01:12:09.88 AS.seek 0ml @01:12:37.24 AFV.select :carrierSolution @01:12:37.38 delay 2 seconds @01:12:39.41 AS.reconfigure ASconfig @01:12:39.48 AS.seek 1ml @01:13:37.23 delay 1 second @01:13:38.24 AFV.select :holdingCoil @01:13:38.37 delay 1 second @01:13:39.40 AS.reconfigure ASfast @01:13:39.47 AS.seek 0ml @01:14:06.79 AFV.select :carrierSolution @01:14:06.93 delay 2 seconds @01:14:08.96 AS.reconfigure ASconfig @01:14:09.03 AS.seek 1ml @01:15:06.80 delay 1 second @01:15:07.81 AFV.select :holdingCoil @01:15:07.94 delay 1 second @01:15:08.97 AS.reconfigure ASfast @01:15:09.04 AS.seek 0ml @01:15:36.41 ARV.dial between carrier and air1 @01:15:36.92 ATV.dial :air3 @01:15:38.26 AS.reconfigure ASslow1 @01:15:38.33 AS.seek 0.01ml @01:15:40.70 delay 2 seconds @01:15:42.72 ATV.dial :bleach @01:15:43.37 AS.seek 0.81ml @01:18:24.56 delay 3 seconds @01:18:27.59 ATV.dial :air3 @01:18:28.17 delay 3 seconds @01:18:31.26 AS.seek 0.835ml @01:18:36.60 delay 3 seconds @01:18:39.63 ATV.dial :bleach @01:18:40.29 AS.seek 0.82ml @01:18:43.61 delay 3 seconds @01:18:46.64 ATV.dial PCR @01:18:47.05 AS.reconfigure ASconfig @01:18:47.12 AS.seek :empty @01:19:34.61 PCR.program :bleachProfile @01:19:34.68 PCR program should complete in < 2:45 @01:19:34.69 PCR.run 10:00 @01:22:30.47 PCR.off @01:22:30.50 AFV.select :carrierSolution @01:22:30.62 delay 2 seconds @01:22:32.65 AS.seek 1ml @01:23:30.45 delay 1 second @01:23:31.46 AFV.select :holdingCoil @01:23:31.59 delay 1 second @01:23:32.62 AS.reconfigure ASfast @01:23:32.69 AS.seek 0ml @01:24:00.05 delay 3 seconds @01:24:03.07 AFV.select :carrierSolution @01:24:03.20 delay 2 seconds @01:24:05.23 AS.reconfigure ASconfig @01:24:05.32 AS.seek 1ml @01:25:03.08 delay 1 second @01:25:04.09 AFV.select :holdingCoil @01:25:04.22 delay 1 second @01:25:05.25 AS.reconfigure ASfast @01:25:05.32 AS.seek 0ml @01:25:32.66 delay 3 seconds @01:25:35.68 AFV.select :carrierSolution @01:25:35.81 delay 2 seconds @01:25:37.84 AS.reconfigure ASconfig @01:25:38.51 AS.seek 1ml @01:26:36.28 delay 1 second @01:26:37.29 AFV.select :holdingCoil @01:26:37.42 delay 1 second @01:26:38.45 AS.reconfigure ASfast @01:26:38.52 AS.seek 0ml @01:27:05.88 delay 3 seconds @01:27:08.90 AFV.select :carrierSolution @01:27:09.03 delay 2 seconds @01:27:11.06 AS.reconfigure ASconfig @01:27:11.13 AS.seek 1ml @01:28:08.95 delay 1 second @01:28:09.96 AFV.select :holdingCoil @01:28:10.09 delay 1 second @01:28:11.12 AS.reconfigure ASfast @01:28:11.19 AS.seek 0ml @01:28:38.58 delay 3 seconds @01:28:41.60 AFV.select :carrierSolution @01:28:41.73 delay 2 seconds @01:28:43.76 AS.reconfigure ASconfig @01:28:43.84 AS.seek 1ml @01:29:41.61 delay 1 second @01:29:42.62 AFV.select :holdingCoil @01:29:42.75 delay 1 second @01:29:43.78 AS.reconfigure ASnorm @01:29:43.85 AS.seek 0ml @01:30:34.58 Setting up PCR for NM -- writing /var/log/esp/NM20may0401h225ml.pcr @01:30:34.60 Building PCR Slug from ASV with MM1 mix, PP1 primer @01:30:34.63 Priming with carrier @01:30:34.66 ATV.dial ARV @01:30:35.30 ARV.dial :carrier @01:30:35.66 AS.seek 0.5ml @01:31:01.23 AFV.select :carrierSolution @01:31:01.36 delay 2 seconds @01:31:03.39 AS.reconfigure ASconfig @01:31:03.46 AS.seek 1ml @01:31:32.52 delay 1 second @01:31:33.53 AFV.select :holdingCoil @01:31:33.66 delay 1 second @01:31:34.71 ATV.dial :waste @01:31:36.42 AS.reconfigure ASnorm @01:31:36.49 AS.seek :empty @01:32:27.27 AFV.select :carrierSolution @01:32:27.41 delay 2 seconds @01:32:29.44 AS.reconfigure ASconfig @01:32:29.51 AS.seek 0.5ml @01:32:58.59 delay 1 second @01:32:59.60 AFV.select :holdingCoil @01:32:59.73 Building Slug @01:32:59.74 ATV.dial :ARV @01:33:01.44 delay 2 seconds @01:33:03.47 ARV.dial :air1 @01:33:03.87 delay 2 seconds @01:33:05.95 AS.reconfigure ASslow2 @01:33:06.03 AS.seek 0.506ml @01:33:12.25 delay 2 seconds @01:33:14.27 ARV.dial :MM1 @01:33:14.89 delay 2 seconds @01:33:16.92 Adding MM1 @01:33:17.01 AS.seek 0.524ml @01:33:35.41 delay 25 seconds @01:34:00.44 ARV.dial :PP1 @01:34:00.84 Adding PP1 @01:34:00.86 delay 2 seconds @01:34:02.96 AS.seek 0.53ml @01:34:09.44 delay 10 seconds @01:34:19.47 ARV.dial :air1 @01:34:19.88 adding ARV air @01:34:19.90 delay 2 seconds @01:34:21.99 AS.seek 0.536ml @01:34:28.28 delay 5 seconds @01:34:33.31 ARV.dial :carrier @01:34:33.72 delay 7 seconds @01:34:40.81 AS.seek 0.594ml @01:35:39.49 delay 5 seconds @01:35:44.52 ATV.dial :ASV @01:35:44.94 delay 2 seconds @01:35:47.03 AS.seek 0.6ml @01:35:53.22 delay 4 seconds @01:35:57.25 ATV.dial ARV @01:35:57.83 delay 2 seconds @01:35:59.86 ARV.dial :carrier @01:35:59.87 delay 2 seconds @01:36:01.97 AS.seek 0.65ml @01:36:52.70 delay 4 seconds @01:36:56.73 ATV.dial PCR @01:36:57.36 ASV.dial :waste1 @01:36:57.38 ARV.dial :air1 @01:36:57.81 Positioning Slug by 0.324ml @01:36:57.92 AS.seek 0.326ml @01:42:24.05 delay 5 seconds @01:42:29.08 ATV.dial ARV @01:42:29.89 ASV.dial :PCRlock @01:42:30.30 delay 5 seconds @01:42:35.33 Running NM Profile60C300 thermocycling @01:42:35.34 PCR.program Profile60C300 @01:42:35.47 Profile60C300 should complete in < 1:59:25 @01:42:35.48 PCR.run 2:34:16.25 @03:18:48.97 PCR.off @03:18:49.67 PCR for NM completed -- closed /var/log/esp/NM20may0401h225ml.pcr @03:18:49.76 Cleaning PCR @03:18:49.77 AFV.select :carrierSolution @03:18:49.91 delay 2 seconds @03:18:51.94 AS.reconfigure ASconfig @03:18:52.01 AS.seek 0.5ml @03:19:02.35 delay 1 second @03:19:03.36 AFV.select :holdingCoil @03:19:03.49 ASV.dial :waste1 @03:19:03.89 ATV.dial :air3 @03:19:05.21 AS.reconfigure ASslow1 @03:19:05.28 AS.seek 0.51ml @03:19:07.57 delay 2 seconds @03:19:09.60 ATV.dial :bleach @03:19:10.25 AS.seek 0.81ml @03:20:10.88 delay 3 seconds @03:20:13.91 ATV.dial :air3 @03:20:14.49 delay 3 seconds @03:20:17.62 AS.seek 0.835ml @03:20:22.97 delay 3 seconds @03:20:26.00 ATV.dial :bleach @03:20:26.64 AS.seek 0.82ml @03:20:29.99 delay 3 seconds @03:20:33.02 ARV.dial :waste @03:20:33.64 delay 3 seconds @03:20:36.67 ATV.dial :ARV @03:20:37.59 AS.reconfigure ASconfig @03:20:37.65 AS.seek 0.47ml @03:20:58.07 AFV.select :carrierSolution @03:20:58.21 delay 2 seconds @03:21:00.24 AS.seek 1ml @03:21:31.02 delay 1 second @03:21:32.03 AFV.select :holdingCoil @03:21:32.16 delay 1 second @03:21:33.19 AS.reconfigure ASfast @03:21:33.26 AS.seek 0ml @03:22:00.62 AFV.select :carrierSolution @03:22:00.75 delay 2 seconds @03:22:02.78 AS.reconfigure ASconfig @03:22:02.85 AS.seek 1ml @03:23:00.61 delay 1 second @03:23:01.62 AFV.select :holdingCoil @03:23:01.75 delay 1 second @03:23:02.78 AS.reconfigure ASfast @03:23:02.85 AS.seek 0ml @03:23:30.20 AFV.select :carrierSolution @03:23:30.34 delay 2 seconds @03:23:32.37 AS.reconfigure ASconfig @03:23:32.44 AS.seek 1ml @03:24:30.27 delay 1 second @03:24:31.28 AFV.select :holdingCoil @03:24:31.41 delay 1 second @03:24:32.44 AS.reconfigure ASfast @03:24:32.51 AS.seek 0ml @03:24:59.86 ARV.dial between carrier and air1 @03:25:00.38 ATV.dial :air3 @03:25:01.70 AS.reconfigure ASslow1 @03:25:01.78 AS.seek 0.01ml @03:25:04.16 delay 2 seconds @03:25:06.18 ATV.dial :bleach @03:25:06.84 AS.seek 0.81ml @03:27:48.02 delay 3 seconds @03:27:51.05 ATV.dial :air3 @03:27:51.63 delay 3 seconds @03:27:54.72 AS.seek 0.835ml @03:28:00.05 delay 3 seconds @03:28:03.07 ATV.dial :bleach @03:28:04.40 AS.seek 0.82ml @03:28:07.74 delay 3 seconds @03:28:10.77 ATV.dial PCR @03:28:11.18 AS.reconfigure ASconfig @03:28:11.25 AS.seek :empty @03:28:58.74 PCR.program :bleachProfile @03:28:58.81 PCR program should complete in < 2:45 @03:28:58.82 PCR.run 10:00 @03:31:54.60 PCR.off @03:31:54.64 AFV.select :carrierSolution @03:31:54.77 delay 2 seconds @03:31:56.80 AS.seek 1ml @03:32:54.61 delay 1 second @03:32:55.62 AFV.select :holdingCoil @03:32:55.75 delay 1 second @03:32:56.78 AS.reconfigure ASfast @03:32:56.85 AS.seek 0ml @03:33:24.21 delay 3 seconds @03:33:27.23 AFV.select :carrierSolution @03:33:27.36 delay 2 seconds @03:33:29.39 AS.reconfigure ASconfig @03:33:29.46 AS.seek 1ml @03:34:27.24 delay 1 second @03:34:28.25 AFV.select :holdingCoil @03:34:28.38 delay 1 second @03:34:29.41 AS.reconfigure ASfast @03:34:29.48 AS.seek 0ml @03:34:56.82 delay 3 seconds @03:34:59.84 AFV.select :carrierSolution @03:34:59.97 delay 2 seconds @03:35:02.00 AS.reconfigure ASconfig @03:35:02.08 AS.seek 1ml @03:35:59.88 delay 1 second @03:36:00.89 AFV.select :holdingCoil @03:36:01.02 delay 1 second @03:36:02.05 AS.reconfigure ASfast @03:36:02.12 AS.seek 0ml @03:36:29.49 delay 3 seconds @03:36:32.51 AFV.select :carrierSolution @03:36:32.64 delay 2 seconds @03:36:34.67 AS.reconfigure ASconfig @03:36:34.75 AS.seek 1ml @03:37:32.54 delay 1 second @03:37:33.55 AFV.select :holdingCoil @03:37:33.68 delay 1 second @03:37:34.71 AS.reconfigure ASfast @03:37:34.78 AS.seek 0ml @03:38:02.13 delay 3 seconds @03:38:05.15 AFV.select :carrierSolution @03:38:05.28 delay 2 seconds @03:38:07.31 AS.reconfigure ASconfig @03:38:07.39 AS.seek 1ml @03:39:05.19 delay 1 second @03:39:06.20 AFV.select :holdingCoil @03:39:06.33 delay 1 second @03:39:07.36 AS.reconfigure ASnorm @03:39:07.43 AS.seek 0ml @03:39:58.20 Setting up PCR for IPc -- writing /var/log/esp/IPc20may0403h225ml.pcr @03:39:58.22 Building PCR Slug from ASV with MM3 mix, PP6 primer @03:39:58.24 Priming with carrier @03:39:58.27 ATV.dial ARV @03:39:58.91 ARV.dial :carrier @03:39:59.28 AS.seek 0.5ml @03:40:24.84 AFV.select :carrierSolution @03:40:24.97 delay 2 seconds @03:40:27.00 AS.reconfigure ASconfig @03:40:27.07 AS.seek 1ml @03:40:56.10 delay 1 second @03:40:57.11 AFV.select :holdingCoil @03:40:57.24 delay 1 second @03:40:58.27 ATV.dial :waste @03:40:59.97 AS.reconfigure ASnorm @03:41:00.04 AS.seek :empty @03:41:50.78 AFV.select :carrierSolution @03:41:50.92 delay 2 seconds @03:41:52.95 AS.reconfigure ASconfig @03:41:53.02 AS.seek 0.5ml @03:42:22.16 delay 1 second @03:42:23.17 AFV.select :holdingCoil @03:42:23.30 Building Slug @03:42:23.31 ATV.dial :ARV @03:42:25.00 delay 2 seconds @03:42:27.03 ARV.dial :air1 @03:42:27.44 delay 2 seconds @03:42:29.52 AS.reconfigure ASslow2 @03:42:29.60 AS.seek 0.506ml @03:42:35.88 delay 2 seconds @03:42:37.90 ARV.dial :MM3 @03:42:38.97 delay 2 seconds @03:42:41.00 Adding MM3 @03:42:41.10 AS.seek 0.524ml @03:42:59.60 delay 25 seconds @03:43:24.62 ARV.dial :PP6 @03:43:25.04 Adding PP6 @03:43:25.06 delay 2 seconds @03:43:27.16 AS.seek 0.53ml @03:43:33.46 delay 10 seconds @03:43:43.49 ARV.dial :air1 @03:43:44.32 adding ARV air @03:43:44.92 delay 2 seconds @03:43:47.03 AS.seek 0.536ml @03:43:53.46 delay 5 seconds @03:43:58.49 ARV.dial :carrier @03:43:58.91 delay 7 seconds @03:44:06.00 AS.seek 0.594ml @03:45:04.75 delay 5 seconds @03:45:09.78 ATV.dial :ASV @03:45:10.21 delay 2 seconds @03:45:12.30 AS.seek 0.6ml @03:45:18.71 delay 4 seconds @03:45:22.74 ATV.dial ARV @03:45:23.43 delay 2 seconds @03:45:25.46 ARV.dial :carrier @03:45:25.47 delay 2 seconds @03:45:27.57 AS.seek 0.65ml @03:46:18.02 delay 4 seconds @03:46:22.05 ATV.dial PCR @03:46:22.69 ASV.dial :waste1 @03:46:22.71 ARV.dial :air1 @03:46:23.13 Positioning Slug by 0.324ml @03:46:23.24 AS.seek 0.326ml @03:51:49.40 delay 5 seconds @03:51:54.43 ATV.dial ARV @03:51:55.22 ASV.dial :PCRlock @03:51:55.63 delay 5 seconds @03:52:00.66 Running IPc ProfileIPc thermocycling @03:52:00.67 PCR.program ProfileIPc @03:52:00.80 ProfileIPc should complete in < 1:59:25 @03:52:00.81 PCR.run 2:34:16.25 @05:29:14.21 PCR.off @05:29:14.89 PCR for IPc completed -- closed /var/log/esp/IPc20may0403h225ml.pcr @05:29:14.98 Cleaning PCR @05:29:14.99 AFV.select :carrierSolution @05:29:15.13 delay 2 seconds @05:29:17.16 AS.reconfigure ASconfig @05:29:17.23 AS.seek 0.5ml @05:29:27.57 delay 1 second @05:29:28.58 AFV.select :holdingCoil @05:29:28.71 ASV.dial :waste1 @05:29:29.10 ATV.dial :air3 @05:29:30.43 AS.reconfigure ASslow1 @05:29:30.51 AS.seek 0.51ml @05:29:32.93 delay 2 seconds @05:29:34.96 ATV.dial :bleach @05:29:35.61 AS.seek 0.81ml @05:30:36.24 delay 3 seconds @05:30:39.27 ATV.dial :air3 @05:30:39.85 delay 3 seconds @05:30:42.94 AS.seek 0.835ml @05:30:48.29 delay 3 seconds @05:30:51.32 ATV.dial :bleach @05:30:51.97 AS.seek 0.82ml @05:30:55.35 delay 3 seconds @05:30:58.38 ARV.dial :waste @05:30:59.62 delay 3 seconds @05:31:02.65 ATV.dial :ARV @05:31:03.56 AS.reconfigure ASconfig @05:31:03.63 AS.seek 0.47ml @05:31:24.04 AFV.select :carrierSolution @05:31:24.18 delay 2 seconds @05:31:26.21 AS.seek 1ml @05:31:56.96 delay 1 second @05:31:57.97 AFV.select :holdingCoil @05:31:58.10 delay 1 second @05:31:59.13 AS.reconfigure ASfast @05:31:59.20 AS.seek 0ml @05:32:26.54 AFV.select :carrierSolution @05:32:26.68 delay 2 seconds @05:32:28.71 AS.reconfigure ASconfig @05:32:28.78 AS.seek 1ml @05:33:26.54 delay 1 second @05:33:27.55 AFV.select :holdingCoil @05:33:27.68 delay 1 second @05:33:28.71 AS.reconfigure ASfast @05:33:28.78 AS.seek 0ml @05:33:56.12 AFV.select :carrierSolution @05:33:56.26 delay 2 seconds @05:33:58.29 AS.reconfigure ASconfig @05:33:58.36 AS.seek 1ml @05:34:56.12 delay 1 second @05:34:57.13 AFV.select :holdingCoil @05:34:57.26 delay 1 second @05:34:58.29 AS.reconfigure ASfast @05:34:58.36 AS.seek 0ml @05:35:25.70 ARV.dial between carrier and air1 @05:35:26.21 ATV.dial :air3 @05:35:27.55 AS.reconfigure ASslow1 @05:35:27.62 AS.seek 0.01ml @05:35:29.93 delay 2 seconds @05:35:31.95 ATV.dial :bleach @05:35:32.61 AS.seek 0.81ml @05:38:13.76 delay 3 seconds @05:38:16.79 ATV.dial :air3 @05:38:17.37 delay 3 seconds @05:38:20.46 AS.seek 0.835ml @05:38:25.78 delay 3 seconds @05:38:28.81 ATV.dial :bleach @05:38:29.45 AS.seek 0.82ml @05:38:32.77 delay 3 seconds @05:38:35.80 ATV.dial PCR @05:38:36.23 AS.reconfigure ASconfig @05:38:36.30 AS.seek :empty @05:39:23.78 PCR.program :bleachProfile @05:39:23.85 PCR program should complete in < 2:45 @05:39:23.87 PCR.run 10:00 @05:42:19.64 PCR.off @05:42:19.68 AFV.select :carrierSolution @05:42:19.79 delay 2 seconds @05:42:21.82 AS.seek 1ml @05:43:19.68 delay 1 second @05:43:20.69 AFV.select :holdingCoil @05:43:20.82 delay 1 second @05:43:21.85 AS.reconfigure ASfast @05:43:21.92 AS.seek 0ml @05:43:49.27 delay 3 seconds @05:43:52.29 AFV.select :carrierSolution @05:43:52.42 delay 2 seconds @05:43:54.45 AS.reconfigure ASconfig @05:43:54.53 AS.seek 1ml @05:44:52.29 delay 1 second @05:44:53.30 AFV.select :holdingCoil @05:44:53.43 delay 1 second @05:44:54.46 AS.reconfigure ASfast @05:44:54.53 AS.seek 0ml @05:45:21.87 delay 3 seconds @05:45:24.89 AFV.select :carrierSolution @05:45:25.02 delay 2 seconds @05:45:27.05 AS.reconfigure ASconfig @05:45:27.12 AS.seek 1ml @05:46:24.88 delay 1 second @05:46:25.89 AFV.select :holdingCoil @05:46:26.02 delay 1 second @05:46:27.05 AS.reconfigure ASfast @05:46:27.12 AS.seek 0ml @05:46:54.46 delay 3 seconds @05:46:57.48 AFV.select :carrierSolution @05:46:57.61 delay 2 seconds @05:46:59.64 AS.reconfigure ASconfig @05:46:59.72 AS.seek 1ml @05:47:57.48 delay 1 second @05:47:58.49 AFV.select :holdingCoil @05:47:58.62 delay 1 second @05:47:59.65 AS.reconfigure ASfast @05:47:59.72 AS.seek 0ml @05:48:27.09 delay 3 seconds @05:48:30.11 AFV.select :carrierSolution @05:48:30.24 delay 2 seconds @05:48:32.27 AS.reconfigure ASconfig @05:48:32.35 AS.seek 1ml @05:49:30.18 delay 1 second @05:49:31.19 AFV.select :holdingCoil @05:49:31.32 delay 1 second @05:49:32.35 AS.reconfigure ASnorm @05:49:32.42 AS.seek 0ml @05:50:23.17 Setting up PCR for IPc2 -- writing /var/log/esp/IPc220may0405h225ml.pcr @05:50:23.20 Building PCR Slug from ASV with MM2 mix, PP3 primer @05:50:23.23 Priming with carrier @05:50:23.84 ATV.dial ARV @05:50:24.51 ARV.dial :carrier @05:50:24.88 AS.seek 0.5ml @05:50:50.47 AFV.select :carrierSolution @05:50:50.61 delay 2 seconds @05:50:52.64 AS.reconfigure ASconfig @05:50:52.72 AS.seek 1ml @05:51:21.79 delay 1 second @05:51:22.80 AFV.select :holdingCoil @05:51:22.93 delay 1 second @05:51:23.96 ATV.dial :waste @05:51:25.65 AS.reconfigure ASnorm @05:51:25.72 AS.seek :empty @05:52:16.47 AFV.select :carrierSolution @05:52:16.61 delay 2 seconds @05:52:18.64 AS.reconfigure ASconfig @05:52:18.71 AS.seek 0.5ml @05:52:47.84 delay 1 second @05:52:48.86 AFV.select :holdingCoil @05:52:48.99 Building Slug @05:52:49.01 ATV.dial :ARV @05:52:50.68 delay 2 seconds @05:52:52.71 ARV.dial :air1 @05:52:53.10 delay 2 seconds @05:52:55.18 AS.reconfigure ASslow2 @05:52:55.26 AS.seek 0.506ml @05:53:01.49 delay 2 seconds @05:53:03.51 ARV.dial :MM2 @05:53:05.02 delay 2 seconds @05:53:07.05 Adding MM2 @05:53:07.14 AS.seek 0.524ml @05:53:25.59 delay 25 seconds @05:53:50.62 ARV.dial :PP3 @05:53:51.02 Adding PP3 @05:53:51.03 delay 2 seconds @05:53:53.13 AS.seek 0.53ml @05:53:59.45 delay 10 seconds @05:54:09.48 ARV.dial :air2 @05:54:09.90 adding ARV air @05:54:09.92 delay 2 seconds @05:54:12.02 AS.seek 0.536ml @05:54:18.41 delay 5 seconds @05:54:23.44 ARV.dial :carrier @05:54:24.74 delay 7 seconds @05:54:31.83 AS.seek 0.594ml @05:55:30.41 delay 5 seconds @05:55:35.44 ATV.dial :ASV @05:55:35.87 delay 2 seconds @05:55:37.96 AS.seek 0.6ml @05:55:44.37 delay 4 seconds @05:55:48.40 ATV.dial ARV @05:55:48.98 delay 2 seconds @05:55:51.01 ARV.dial :carrier @05:55:51.03 delay 2 seconds @05:55:53.13 AS.seek 0.65ml @05:56:43.65 delay 4 seconds @05:56:47.68 ATV.dial PCR @05:56:48.31 ASV.dial :waste1 @05:56:48.33 ARV.dial :air1 @05:56:48.77 Positioning Slug by 0.324ml @05:56:48.88 AS.seek 0.326ml @06:02:15.29 delay 5 seconds @06:02:20.32 ATV.dial ARV @06:02:21.12 ASV.dial :PCRlock @06:02:21.52 delay 5 seconds @06:02:26.55 Running IPc2 ProfileIPc thermocycling @06:02:26.56 PCR.program ProfileIPc @06:02:26.68 ProfileIPc should complete in < 1:59:25 @06:02:26.70 PCR.run 2:34:16.25 @07:40:03.80 PCR.off @07:40:04.50 PCR for IPc2 completed -- closed /var/log/esp/IPc220may0405h225ml.pcr @07:40:04.52 Full MFB Decontamination @07:40:04.54 Decon extraction column and decon AIV @07:40:04.58 SPE.hold 2:46:40,55C @07:40:04.65 ASV.dial :SPEdelivery @07:40:05.32 delay 20 seconds @07:40:25.34 AFV.select :carrierSolution @07:40:25.47 delay 2 seconds @07:40:27.50 AS.reconfigure ASconfig @07:40:27.57 AS.seek 0.5ml @07:40:37.93 delay 1 second @07:40:38.95 AFV.select :holdingCoil @07:40:39.07 ATV.dial :air1 @07:40:41.10 AS.reconfigure ASslow1 @07:40:41.18 AS.seek 0.52ml @07:40:45.52 ATV.dial :ASV @07:40:47.42 ASV.dial :SPErecovery @07:40:48.14 AS.seek 0.62ml @07:41:08.53 ATV.dial :waste @07:41:10.67 AS.reconfigure ASnorm @07:41:10.74 AS.seek :empty @07:41:42.39 AFV.select :holdingCoil @07:41:42.41 ATV.dial :waste @07:41:42.45 AS.reconfigure ASconfig @07:41:42.52 AS.seek 0ml @07:41:42.83 AFV.select :carrierSolution @07:41:42.97 delay 2 seconds @07:41:45.00 AS.seek 0.5ml @07:42:14.04 delay 1 second @07:42:15.05 AFV.select :holdingCoil @07:42:15.18 ATV.dial :air3 @07:42:15.94 AS.reconfigure ASslow1 @07:42:16.01 AS.seek 0.52ml @07:42:20.34 ATV.dial :air3 @07:42:20.44 AS.seek 0.53ml @07:42:22.73 delay 2 seconds @07:42:24.75 ATV.dial :bleach @07:42:25.29 AS.seek 0.71ml @07:43:01.78 delay 3 seconds @07:43:04.81 ATV.dial :air3 @07:43:05.42 delay 3 seconds @07:43:08.51 AS.seek 0.735ml @07:43:13.85 delay 3 seconds @07:43:16.88 ATV.dial :bleach @07:43:17.57 AS.seek 0.72ml @07:43:20.92 delay 3 seconds @07:43:23.95 ATV.dial ASV @07:43:25.09 ASV.dial :SPErecovery @07:43:25.19 AS.seek 0.365ml @07:44:36.86 ATV.dial :waste @07:44:38.70 AS.reconfigure ASnorm @07:44:38.78 AS.seek :empty @07:44:57.57 ATV.dial :air3 @07:44:58.29 AS.reconfigure ASslow1 @07:44:58.36 AS.seek 0.009ml @07:45:00.61 delay 2 seconds @07:45:02.63 ATV.dial :bleach @07:45:03.14 AS.seek 0.579ml @07:46:58.09 delay 3 seconds @07:47:01.12 ATV.dial :air3 @07:47:01.72 delay 3 seconds @07:47:04.81 AS.seek 0.604ml @07:47:10.14 delay 3 seconds @07:47:13.17 ATV.dial :bleach @07:47:13.82 AS.seek 0.589ml @07:47:17.15 delay 3 seconds @07:47:20.18 ATV.dial :ASV @07:47:21.26 ASV.dial :wasteR @07:47:21.69 AS.seek 0ml @07:49:21.04 delay 1:00 @07:50:21.07 ATV.dial ASV @07:50:21.08 AFV.select :carrierSolution @07:50:21.21 delay 2 seconds @07:50:23.24 AS.reconfigure ASconfig @07:50:23.31 AS.seek 1ml @07:51:21.12 delay 1 second @07:51:22.14 AFV.select :holdingCoil @07:51:22.26 delay 1 second @07:51:23.29 ASV.dial :wasteR @07:51:23.31 AS.reconfigure ASslow1 @07:51:23.39 AS.seek 0ml @07:54:44.81 delay 10 seconds @07:54:54.84 ATV.dial :air3 @07:54:56.39 AS.seek 0.01ml @07:54:58.81 delay 2 seconds @07:55:00.84 ATV.dial :bleach @07:55:01.49 AS.seek 0.58ml @07:56:56.53 delay 3 seconds @07:56:59.56 ATV.dial :air3 @07:57:00.14 delay 3 seconds @07:57:03.23 AS.seek 0.605ml @07:57:08.63 delay 3 seconds @07:57:11.66 ATV.dial :bleach @07:57:12.32 AS.seek 0.59ml @07:57:15.72 delay 3 seconds @07:57:18.75 ATV.dial ASV @07:57:19.84 ASV.dial :SPEdelivery @07:57:20.24 AS.seek 0ml @07:59:19.18 delay 1:00 @08:00:19.21 AFV.select :carrierSolution @08:00:19.33 delay 2 seconds @08:00:21.36 AS.reconfigure ASconfig @08:00:21.43 AS.seek 1ml @08:01:19.22 delay 1 second @08:01:20.23 AFV.select :holdingCoil @08:01:20.36 delay 1 second @08:01:21.39 ATV.dial ASV @08:01:21.40 ASV.dial :SPEdelivery @08:01:21.44 AS.reconfigure ASslow1 @08:01:21.51 AS.seek 0ml @08:04:42.92 delay 10 seconds @08:04:52.95 ATV.dial :air2 @08:04:53.79 AS.reconfigure ASslow0 @08:04:53.87 AS.seek 0.8ml @08:06:14.68 delay 3 seconds @08:06:17.71 ATV.dial :ASV @08:06:18.73 ASV.dial :SPEdelivery @08:06:18.75 AS.reconfigure ASslow1 @08:06:18.83 AS.seek 0.03ml @08:08:53.98 delay 20 seconds @08:09:14.01 ATV.dial :waste,via: :air3 @08:09:15.79 AS.reconfigure ASconfig @08:09:15.86 AS.seek :empty @08:09:17.94 SPE.coast @08:09:18.05 Removing Bleach from Mixing Coil @08:09:18.06 AFV.select :carrierSolution @08:09:18.19 delay 2 seconds @08:09:20.22 AS.seek 0.5ml @08:09:49.31 delay 1 second @08:09:50.32 AFV.select :holdingCoil @08:09:50.45 ATV.dial :ASV @08:09:52.17 ASV.dial :SPErecovery @08:09:52.85 AS.reconfigure ASslow1 @08:09:52.92 AS.seek 0.73ml @08:10:39.47 ASV.dial :wasteD @08:10:40.09 AS.reconfigure ASnorm @08:10:40.16 AS.seek 0ml @08:11:17.30 AFV.select :carrierSolution @08:11:17.44 delay 2 seconds @08:11:19.47 AS.reconfigure ASconfig @08:11:19.54 AS.seek 0.5ml @08:11:48.59 delay 1 second @08:11:49.60 AFV.select :holdingCoil @08:11:49.73 ATV.dial ASV @08:11:49.75 ASV.dial :SPErecovery @08:11:50.43 AS.reconfigure ASslow0 @08:11:50.50 AS.seek 0.3ml @08:12:10.97 ATV.dial :air2 @08:12:12.05 AS.reconfigure ASslow1 @08:12:12.12 AS.seek 0.32ml @08:12:16.47 ATV.dial ASV @08:12:17.55 AS.reconfigure ASslow0 @08:12:17.62 AS.seek 0.57ml @08:12:43.13 ASV.dial :wasteD @08:12:43.75 AS.reconfigure ASnorm @08:12:43.83 AS.seek 0ml @08:13:12.89 AFV.select :carrierSolution @08:13:13.03 delay 2 seconds @08:13:15.06 AS.reconfigure ASconfig @08:13:15.13 AS.seek 0.5ml @08:13:44.18 delay 1 second @08:13:45.19 AFV.select :holdingCoil @08:13:45.32 ATV.dial ASV @08:13:45.33 ASV.dial :SPErecovery @08:13:46.58 AS.reconfigure ASslow0 @08:13:46.68 AS.seek 0.3ml @08:14:07.14 ATV.dial :air2 @08:14:08.22 AS.reconfigure ASslow1 @08:14:08.29 AS.seek 0.32ml @08:14:12.65 ATV.dial ASV @08:14:13.75 AS.reconfigure ASslow0 @08:14:13.82 AS.seek 0.57ml @08:14:39.30 ASV.dial :wasteD @08:14:39.92 AS.reconfigure ASnorm @08:14:40.00 AS.seek 0ml @08:15:09.08 ATV.dial :air2 @08:15:10.09 AS.reconfigure ASslow0 @08:15:10.17 AS.seek 0.3ml @08:15:40.77 ATV.dial :ASV @08:15:41.79 ASV.dial :wasteD @08:15:41.81 AS.reconfigure ASconfig @08:15:41.89 AS.seek 0.05ml @08:15:56.58 ATV.dial :waste @08:15:58.51 AS.seek 0ml @08:16:01.67 ASV.dial :SPEdelivery @08:16:02.70 deconLysateINT @08:16:02.71 ATV.dial :air3 @08:16:03.43 AS.reconfigure ASslow1 @08:16:03.50 AS.seek 0.01ml @08:16:05.82 delay 2 seconds @08:16:07.84 ATV.dial :bleach @08:16:08.32 AS.seek 0.96ml @08:19:19.64 delay 3 seconds @08:19:22.67 ATV.dial :air3 @08:19:23.25 delay 3 seconds @08:19:26.34 AS.seek 0.985ml @08:19:31.66 delay 3 seconds @08:19:34.69 ATV.dial :bleach @08:19:35.35 AS.seek 0.97ml @08:19:38.65 delay 3 seconds @08:19:41.68 ATV.dial AIV @08:19:42.95 AIV.dial :coilToMFB @08:19:42.97 AWV.select :waste @08:19:42.99 AFV.select :carrierSolution @08:19:43.13 delay 2 seconds @08:19:45.16 AS.reconfigure ASconfig @08:19:45.23 AS.seek 1ml @08:19:47.28 delay 1 second @08:19:48.29 AFV.select :holdingCoil @08:19:48.42 delay 1 second @08:19:49.45 AS.reconfigure ASslow0 @08:19:49.51 AS.seek :empty @08:21:30.53 AFV.select :carrierSolution @08:21:30.67 delay 2 seconds @08:21:32.70 AS.reconfigure ASconfig @08:21:32.77 AS.seek 1ml @08:22:30.57 delay 1 second @08:22:31.58 AFV.select :holdingCoil @08:22:31.71 delay 1 second @08:22:32.74 AS.reconfigure ASslow0 @08:22:32.80 AS.seek :empty @08:24:13.85 AFV.select :carrierSolution @08:24:13.99 delay 2 seconds @08:24:16.02 AS.reconfigure ASconfig @08:24:16.09 AS.seek 1ml @08:25:13.97 delay 1 second @08:25:14.98 AFV.select :holdingCoil @08:25:15.11 delay 1 second @08:25:16.14 AS.reconfigure ASslow0 @08:25:16.20 AS.seek :empty @08:26:57.22 ATV.dial :air2 @08:26:57.94 AS.reconfigure ASslow1 @08:26:58.01 AS.seek 0.009ml @08:27:00.32 AS.reconfigure ASslow0 @08:27:00.39 AS.seek 1ml @08:28:40.33 ATV.dial AIV @08:28:41.15 AS.seek 0.02ml @08:30:20.05 ATV.dial :waste @08:30:21.75 AS.seek 0ml @08:30:24.08 AWV.select :air @08:30:24.27 Cleaning PCR @08:30:24.28 AFV.select :carrierSolution @08:30:24.41 delay 2 seconds @08:30:26.44 AS.reconfigure ASconfig @08:30:26.51 AS.seek 0.5ml @08:30:55.56 delay 1 second @08:30:56.57 AFV.select :holdingCoil @08:30:56.70 ASV.dial :waste1 @08:30:57.07 ATV.dial :air3 @08:30:57.77 AS.reconfigure ASslow1 @08:30:57.84 AS.seek 0.51ml @08:31:00.15 delay 2 seconds @08:31:02.17 ATV.dial :bleach @08:31:03.23 AS.seek 0.81ml @08:32:03.84 delay 3 seconds @08:32:06.87 ATV.dial :air3 @08:32:07.45 delay 3 seconds @08:32:10.54 AS.seek 0.835ml @08:32:15.88 delay 3 seconds @08:32:18.91 ATV.dial :bleach @08:32:19.57 AS.seek 0.82ml @08:32:22.89 delay 3 seconds @08:32:25.92 ARV.dial :waste @08:32:26.54 delay 3 seconds @08:32:29.57 ATV.dial :ARV @08:32:30.46 AS.reconfigure ASconfig @08:32:30.53 AS.seek 0.47ml @08:32:50.97 AFV.select :carrierSolution @08:32:51.11 delay 2 seconds @08:32:53.15 AS.seek 1ml @08:33:23.95 delay 1 second @08:33:24.97 AFV.select :holdingCoil @08:33:25.09 delay 1 second @08:33:26.12 AS.reconfigure ASfast @08:33:26.19 AS.seek 0ml @08:33:53.56 AFV.select :carrierSolution @08:33:53.70 delay 2 seconds @08:33:55.73 AS.reconfigure ASconfig @08:33:55.80 AS.seek 1ml @08:34:53.62 delay 1 second @08:34:54.63 AFV.select :holdingCoil @08:34:54.76 delay 1 second @08:34:55.79 AS.reconfigure ASfast @08:34:55.86 AS.seek 0ml @08:35:23.22 AFV.select :carrierSolution @08:35:23.36 delay 2 seconds @08:35:25.39 AS.reconfigure ASconfig @08:35:25.46 AS.seek 1ml @08:36:23.26 delay 1 second @08:36:24.27 AFV.select :holdingCoil @08:36:24.40 delay 1 second @08:36:25.43 AS.reconfigure ASfast @08:36:25.50 AS.seek 0ml @08:36:52.89 ARV.dial between carrier and air1 @08:36:53.40 ATV.dial :air3 @08:36:54.72 AS.reconfigure ASslow1 @08:36:54.79 AS.seek 0.01ml @08:36:57.17 delay 2 seconds @08:36:59.19 ATV.dial :bleach @08:36:59.85 AS.seek 0.81ml @08:39:41.00 delay 3 seconds @08:39:44.03 ATV.dial :air3 @08:39:44.61 delay 3 seconds @08:39:47.70 AS.seek 0.835ml @08:39:52.96 delay 3 seconds @08:39:55.99 ATV.dial :bleach @08:39:56.64 AS.seek 0.82ml @08:39:59.97 delay 3 seconds @08:40:03.00 ATV.dial PCR @08:40:03.42 AS.reconfigure ASconfig @08:40:03.49 AS.seek :empty @08:40:50.96 PCR.program :bleachProfile @08:40:51.03 PCR program should complete in < 2:45 @08:40:51.05 PCR.run 10:00 @08:43:52.88 PCR.off @08:43:52.92 AFV.select :carrierSolution @08:43:53.05 delay 2 seconds @08:43:55.08 AS.seek 1ml @08:44:52.95 delay 1 second @08:44:53.96 AFV.select :holdingCoil @08:44:54.09 delay 1 second @08:44:55.12 AS.reconfigure ASfast @08:44:55.19 AS.seek 0ml @08:45:22.54 delay 3 seconds @08:45:25.56 AFV.select :carrierSolution @08:45:25.69 delay 2 seconds @08:45:27.72 AS.reconfigure ASconfig @08:45:27.79 AS.seek 1ml @08:46:25.57 delay 1 second @08:46:26.58 AFV.select :holdingCoil @08:46:26.71 delay 1 second @08:46:27.74 AS.reconfigure ASfast @08:46:27.81 AS.seek 0ml @08:46:55.17 delay 3 seconds @08:46:58.19 AFV.select :carrierSolution @08:46:58.32 delay 2 seconds @08:47:00.36 AS.reconfigure ASconfig @08:47:00.44 AS.seek 1ml @08:47:58.20 delay 1 second @08:47:59.21 AFV.select :holdingCoil @08:47:59.34 delay 1 second @08:48:00.37 AS.reconfigure ASfast @08:48:00.44 AS.seek 0ml @08:48:27.79 delay 3 seconds @08:48:30.81 AFV.select :carrierSolution @08:48:30.94 delay 2 seconds @08:48:32.97 AS.reconfigure ASconfig @08:48:33.12 AS.seek 1ml @08:49:30.89 delay 1 second @08:49:31.90 AFV.select :holdingCoil @08:49:32.03 delay 1 second @08:49:33.06 AS.reconfigure ASfast @08:49:33.13 AS.seek 0ml @08:50:00.50 delay 3 seconds @08:50:03.52 AFV.select :carrierSolution @08:50:03.65 delay 2 seconds @08:50:05.68 AS.reconfigure ASconfig @08:50:05.76 AS.seek 1ml @08:51:03.54 delay 1 second @08:51:04.55 AFV.select :holdingCoil @08:51:04.68 delay 1 second @08:51:05.71 AS.reconfigure ASnorm @08:51:05.78 AS.seek 0ml @08:51:56.54 AFV.select :carrierSolution @08:51:56.67 delay 2 seconds @08:51:58.70 AS.reconfigure ASconfig @08:51:58.77 AS.seek 1ml @08:52:56.57 delay 1 second @08:52:57.58 AFV.select :holdingCoil @08:52:57.71 delay 1 second @08:52:58.74 ATV.dial :waste @08:52:59.98 AS.reconfigure ASnorm @08:53:00.06 AS.seek 0.5ml @08:53:25.61 ATV.dial :waste2 @08:53:26.94 AS.seek 0ml @08:53:52.50
Asleep after completing 20invasivedeploymfbtest assays @08:53:52.53 Mission runtime was 15:50:27.748284 @08:53:52.55 GoodNews.email "{1 => 6, 2 => 0}\nCan@08:53:24, 21.6C, 31% humidity, 14.6psia, 11.996V, 0.594A, 0.594A avg, 7.13W",Subject: "Asleep after completing 20invasivedeploymfbtest assays" @08:54:02.75 Session Ended @16:21:36.92CEST04-May-20
ESP2 v3.30 I2C Gateway v3.06 for core bus @16:21:39.59 Copyright 2018 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @16:21:39.89 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @16:21:47.15 Polling Can every 10:00 @16:21:49.19 SPE.coast @16:21:49.32 SPE.configure SPEconfig @16:21:49.51 AS.reconfigure ASconfig @16:21:49.60 AS.hold @16:21:49.71 AS.configure ASconfig @16:21:49.83 SS.reconfigure SSconfig @16:21:49.88 SS.hold @16:21:49.93 SS.configure SSconfig @16:21:50.02 CC.reconfigure CCconfig @16:21:50.08 CC.hold @16:21:50.20 CC.configure CCconfig @16:21:50.29 CS.reconfigure CSconfig @16:21:50.37 CS.hold @16:21:50.49 CS.configure CSconfig @16:21:51.36 PC.reconfigure PCconfig @16:21:51.41 PC.hold @16:21:51.48 PC.configure PCconfig @16:21:51.58 PS.configure PSconfig @16:21:51.70 Forearm.reconfigure LooseForearm @16:21:51.74 Forearm.hold @16:21:51.80 Forearm.configure LooseForearm @16:21:51.90 Elbow.reconfigure LooseElbow @16:21:51.94 Elbow.hold @16:21:52.01 Elbow.configure LooseElbow @16:21:52.26 SC.reconfigure SCloose @16:21:52.35 SC.hold @16:21:52.45 SC.configure SCloose @16:21:52.65 SE.reconfigure SEconfig @16:21:52.73 SE.hold @16:21:52.85 SE.configure SEconfig @16:21:52.94 CH.coast @16:21:53.01 CH.configure CHconfig @16:21:53.04 PH.coast @16:21:53.10 PH.configure PHconfig @16:21:53.14 PCR.off @16:21:53.17 PCR.pidtemp= 50,0.1,0,300,25,25 @16:21:53.20 PCR.caltemp= 2.8814,0.0372,0 @16:21:53.23 All dwarf controllers running firmware version 3.75 @16:21:53.37 Loading /home/esp/esp2/mission/20invasivedeploymfbtest.rb mission @16:21:53.46 Preparing to sleep until +0:00:10 @16:21:53.48 GoodNews.email "Starting 20invasivedeploymfbtest w/pucks in tube 2\n{}\nCan@16:21:47, 21.3C, 30% humidity, 14.6psia, 11.937V, 0.752A, 0.758A avg, 8.98W",Subject: "20invasivedeploymfbtest begun" @16:21:53.63 AS.coast @16:21:53.69
Asleep til +0:00:10 @16:21:53.75 Continuing at 16:22 @16:21:53.83 SS.coast @16:21:54.01 CC.coast @16:21:54.13 CS.coast @16:21:54.21 PC.coast @16:21:54.28 Forearm.coast @16:21:54.35 Elbow.coast @16:21:54.43 SC.coast @16:21:54.59 SE.coast @16:21:54.64 Session Begun @16:21:55.15 Gate.power :core,:OFF @16:21:55.17 Gate.power :raw,:OFF @16:21:55.20 Shutdown @16:22:02.01 GoodNews.email "Then, in tube 2: mbaPhase\n/home/esp/esp2/mission/20invasivedeploymfbtest.rb:119\n{}\nCan@16:21:53, 21.3C, 30% humidity, 14.6psia, 11.923V, 0.796A, 0.796A avg, 9.49W",Subject: "Asleep until 16:22" @16:22:03.47
Powering core bus @16:22:08.48 Gate.power :raw,:ON @16:22:10.27 SPE.coast @16:22:10.54 SPE.configure SPEconfig @16:22:10.85 AS.configure ASconfig @16:22:11.23 SS.configure SSconfig @16:22:11.62 CC.configure CCconfig @16:22:11.99 CS.configure CSconfig @16:22:12.35 PC.configure PCconfig @16:22:12.78 PS.configure PSconfig @16:22:13.31 Forearm.configure LooseForearm @16:22:13.67 Elbow.configure LooseElbow @16:22:14.13 SC.configure SCloose @16:22:14.50 SE.configure SEconfig @16:22:14.77 CH.coast @16:22:15.03 CH.configure CHconfig @16:22:15.16 PH.coast @16:22:15.46 PH.configure PHconfig @16:22:15.60 PCR.off @16:22:15.70 PCR.pidtemp= 50,0.1,0,300,25,25 @16:22:15.84 PCR.caltemp= 2.8814,0.0372,0 @16:22:16.00 All dwarf controllers running firmware version 3.75 @16:22:16.26 Valve.reset @16:22:16.76 PCR.off @16:22:16.89 PCR.pidtemp= 50,0.1,0,300,25,25 @16:22:17.01 PCR.caltemp= 2.8814,0.0372,0 @16:22:17.77 Intake.select :close @16:22:17.80 Exhaust.select :close @16:22:17.94 CTV.dial :air @16:22:18.29 PSV.dial :air @16:22:18.40 CSV.dial :waste1 @16:22:19.13 SSV.dial :spit,avoiding: :exhaust @16:22:19.29 PS.reconfigure PSconfig @16:22:20.49
Forearm.reconfigure LooseForearm @16:22:21.19 Forearm.home.seek :retract @16:22:21.31 PS.home.seek 0ml @16:22:21.96 CS.reconfigure CSfast @16:22:22.06 SS.reconfigure SSconfig @16:22:22.43 SS.home.seek 0ml @16:22:22.60 CS.home.seek 0ml @16:22:22.96 Hand.close @16:22:23.07
Elbow.jog 1500 @16:22:23.76 Elbow.reconfigure LooseElbow @16:22:23.99 Elbow.home.seek :clear @16:22:29.01 SE.reconfigure SEconfig @16:22:29.22 SE.home.seek :down @16:22:30.08 SC.reconfigure SCsloppy @16:22:30.27 SC.home.seek :initial @16:22:30.75 PS.coast @16:22:34.48
Forearm.seek :retract @16:22:34.81 Elbow.seek :hammer @16:22:36.21 Forearm.reconfigure TightForearm @16:22:36.42 Forearm.seek 5 + 350 counts @16:22:37.81 Forearm.seek :retract @16:22:39.64 PC.reconfigure PCconfig @16:22:39.70
CC.reconfigure CCconfig @16:22:40.05 PC.home.seek :open @16:22:40.15
CC.home.seek :open @16:22:44.58 Phase [mbaPhase] @16:22:44.64 GoodNews.email "At 16:22:44 began in tube 2: mbaPhase\n/home/esp/esp2/mission/20invasivedeploymfbtest.rb:119\n{}\nCan@16:22:44, 21.3C, 31% humidity, 14.6psia, 11.942V, 0.752A, 0.752A avg, 8.98W",Subject: "Started 20invasivedeploymfbtest phase mbaPhase" @16:22:44.82CEST04-May-20
===> STARTED Superphase (phase #1) <=== Everybody has a plan untill they get punched in the face - Mike Tyson @16:22:45.17 Starting mfb @16:22:45.22 pcrBAC sampling at most 1500ml, wcr at most 1500ml @16:22:45.48 Loading sh1 puck from tube 2 into Collection Clamp @16:22:45.88 Readying the MFB @16:22:45.97
Hand.open @16:22:46.06 Homing MFB Syringe @16:22:46.17 AFV.select :holdingCoil @16:22:46.63 ATV.dial :air2 @16:22:47.77
SC.reconfigure SCloose @16:22:48.32 AS.reconfigure ASconfig @16:22:48.69 AS.home.seek :home @16:22:49.50
Elbow.reconfigure TightElbow @16:22:49.83 Elbow.seek 2 @16:22:51.07 Forearm.seek 2 + 700 counts @16:22:52.62 SE.reconfigure SEpick @16:22:52.94 SE.seek beamBreak @16:22:54.58 AFV.select :carrierSolution @16:22:54.90 delay 2 seconds @16:22:55.33 BACfans.email "Started mfb in tube 2 at 16:22:45CEST04-May-20\n{}\nCan@16:22:44, 21.3C, 31% humidity, 14.6psia, 11.942V, 0.752A, 0.752A avg, 8.98W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" @16:22:56.63
SE.configure SEconfig @16:22:57.67 AS.seek 1ml @16:22:58.42
Elbow.seek 2 @16:23:01.01 Forearm.seek 2 + 300 counts @16:23:01.63 Forearm.reconfigure LooseForearm @16:23:01.98 Hand.close @16:23:03.01 SE.seek :down @16:23:04.34
Forearm.seek :retract @16:23:05.68 Elbow.seek CC @16:23:06.25 SE.coast @16:23:07.93
Elbow.reconfigure LooseElbow @16:23:08.48 Forearm.reconfigure TightForearm @16:23:08.76 Forearm.seek CC @16:23:11.19 CC.seek :closed @16:23:21.16 Clamp::VelocityError in MAIN -- Collection Clamp always accelerating!?! @16:23:21.21 Retry #1 of 2 @16:23:21.64 I2C::UnexpectedReply in core -- I2C::Servo::Status3Reply[6F:27->20] @16:23:22.50 CC.seek :open @16:23:27.33 CC.seek :closed @16:23:36.15 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.72 @16:23:36.21 Hand.open @16:23:36.55 I2C::UnexpectedReply in core -- I2C::Servo::Status3Reply[75:27->20] @16:23:37.38 Forearm.reconfigure LooseForearm @16:23:37.61 Forearm.seek :retract @16:23:38.76 SC.coast @16:23:38.94 collectSample 1500ml w/bubble point of 55psi @16:23:38.99 Priming 7ml @16:23:39.06 Intake.select :close @16:23:39.10 Exhaust.select :close @16:23:39.19 SS.hold @16:23:40.00 Calibrating pressure and temperature sensors @16:23:40.06 CTV.dial :air @16:23:40.15 SSV.dial :spit,avoiding: :exhaust @16:23:40.27 SS.reconfigure SSfast @16:23:40.51 SS.seek 5ml @16:23:43.76 SSV.dial CBV @16:23:43.88
CBV.dial SSV @16:23:45.61 delay 3.8 seconds @16:23:49.80 SSV.dial :spit @16:23:50.87 delay 1 second @16:23:51.51
SS.seek 0ml @16:23:51.91 AFV.select :holdingCoil @16:23:52.13 delay 1 second @16:23:53.18 ATV.dial :waste @16:23:54.50
SSV.dial CBV @16:23:54.78 AS.reconfigure ASnorm @16:23:54.99 AS.home.seek :empty @16:23:56.19
CTV.dial :intake @16:23:57.26 SSV.dial CBV @16:23:57.35
CBV.dial SSV @16:23:57.45 Allowing 40s for 7.0ml of remaining 7.0ml @16:23:57.53 Intake.select :open @16:24:00.01 Intake @15.8psia, vacuum from 9.3 to 13.3psi @16:24:00.07 SS.reconfigure SSinhale.with maxSpeed: 150 @16:24:00.32 SS.seek 7ml @16:24:05.99 Stroke completed -- waiting for pressure to equalize ... @16:24:21.66 Intake.select :close @16:24:21.84 SSV.dial :exhaust @16:24:22.72 Exhaust.select :open @16:24:22.87 SS.reconfigure SSexhale @16:24:22.94 SS.seek 0ml @16:24:27.22 Exhaust.select :close @16:24:27.35 Sampling 1500.0ml @16:24:27.37 Intake.select :close @16:24:27.39 Exhaust.select :close @16:24:27.42 SS.reconfigure SSconfig @16:24:27.50 SS.hold @16:24:27.73 CTV.dial :intake @16:24:27.75 SSV.dial CBV @16:24:27.79
CBV.dial SSV @16:24:28.69 Allowing 130s for 25.0ml of remaining 1500.0ml @16:24:28.70 Intake.select :open @16:24:30.92 Intake @15.9psia, vacuum from 9.4 to 13.4psi @16:24:30.94 SS.reconfigure SSinhale.with maxSpeed: 150 @16:24:31.03 SS.seek 25ml @16:24:34.68 Vacuum too high -- waiting up to 40s @16:24:40.10 Intake @15.7psia, vacuum from 9.2 to 13.2psi @16:24:40.12 SS.reconfigure SSinhale.with maxSpeed: 135 @16:24:40.21 SS.seek 25ml @16:24:42.81 Vacuum too high -- waiting up to 40s @16:24:46.39 ATV.dial :waste @16:24:46.41 ARV.dial between carrier and air1 @16:24:46.52 ASV.dial :waste1 @16:24:46.79
Intake @16.0psia, vacuum from 9.5 to 13.5psi @16:24:46.81 SS.reconfigure SSinhale.with maxSpeed: 122 @16:24:46.90 SS.seek 25ml @16:24:49.73 Vacuum too high -- waiting up to 40s @16:24:54.40 Intake @16.1psia, vacuum from 9.6 to 13.6psi @16:24:54.42 SS.reconfigure SSinhale.with maxSpeed: 110 @16:24:54.51 SS.seek 25ml @16:24:57.48 Vacuum too high -- waiting up to 40s @16:25:02.07 Intake @16.0psia, vacuum from 9.5 to 13.5psi @16:25:02.09 SS.reconfigure SSinhale.with maxSpeed: 99 @16:25:02.18 SS.seek 25ml @16:25:05.09 Vacuum too high -- waiting up to 40s @16:25:10.16 Intake @16.1psia, vacuum from 9.6 to 13.6psi @16:25:10.19 SS.reconfigure SSinhale.with maxSpeed: 89 @16:25:10.27 SS.seek 25ml @16:25:13.33 Vacuum too high -- waiting up to 40s @16:25:17.76 Intake @16.1psia, vacuum from 9.6 to 13.6psi @16:25:17.78 SS.reconfigure SSinhale.with maxSpeed: 80 @16:25:17.86 SS.seek 25ml @16:25:21.24 Vacuum too high -- waiting up to 40s @16:25:25.81 Intake @16.1psia, vacuum from 9.6 to 13.6psi @16:25:25.84 SS.reconfigure SSinhale.with maxSpeed: 72 @16:25:25.92 SS.seek 25ml @16:25:29.70 Vacuum too high -- waiting up to 40s @16:25:34.78 Intake @16.2psia, vacuum from 9.7 to 13.7psi @16:25:34.81 SS.reconfigure SSinhale.with maxSpeed: 65 @16:25:34.89 SS.seek 25ml @16:25:36.90 Stroke completed -- waiting for pressure to equalize ... @16:25:42.46 Intake.select :close @16:25:42.64 SSV.dial :exhaust @16:25:43.52 Exhaust.select :open @16:25:43.66 SS.reconfigure SSexhale @16:25:43.72 SS.seek 0ml @16:25:53.44 Exhaust.select :close @16:25:53.57 SSV.dial CBV @16:25:53.60
CBV.dial SSV @16:25:54.50 Allowing 130s for 25.0ml of remaining 1475.0ml @16:25:54.51 Intake.select :open @16:25:56.73 Intake @16.0psia, vacuum from 9.5 to 13.5psi @16:25:56.75 SS.reconfigure SSinhale.with maxSpeed: 65 @16:25:56.83 SS.seek 25ml @16:26:00.58 Vacuum too high -- waiting up to 40s @16:26:04.16 Intake @15.8psia, vacuum from 9.3 to 13.3psi @16:26:04.19 SS.reconfigure SSinhale.with maxSpeed: 59 @16:26:04.27 SS.seek 25ml @16:26:08.21 Vacuum too high -- waiting up to 40s @16:26:12.27 Intake @16.0psia, vacuum from 9.5 to 13.5psi @16:26:12.29 SS.reconfigure SSinhale.with maxSpeed: 53 @16:26:12.38 SS.seek 25ml @16:26:17.30 Vacuum too high -- waiting up to 40s @16:26:21.62 Intake @16.1psia, vacuum from 9.6 to 13.6psi @16:26:21.64 SS.reconfigure SSinhale.with maxSpeed: 48 @16:26:21.73 SS.seek 25ml @16:26:28.17 Vacuum too high -- waiting up to 40s @16:26:33.54 Intake @16.1psia, vacuum from 9.6 to 13.6psi @16:26:33.56 SS.reconfigure SSinhale.with maxSpeed: 43 @16:26:33.65 SS.seek 25ml @16:26:40.92 Vacuum too high -- waiting up to 40s @16:26:46.50 Intake @16.1psia, vacuum from 9.6 to 13.6psi @16:26:46.53 SS.reconfigure SSinhale.with maxSpeed: 39 @16:26:46.61 SS.seek 25ml @16:26:52.67 Vacuum too high -- waiting up to 40s @16:26:57.25 SS.reconfigure SSinhale.with maxSpeed: 35 @16:26:57.32 SS.seek 25ml @16:27:07.79 Stroke completed -- waiting for pressure to equalize ... @16:27:17.34 Intake.select :close @16:27:17.51 SSV.dial :exhaust @16:27:18.42 Exhaust.select :open @16:27:18.56 SS.reconfigure SSexhale @16:27:18.63 SS.seek 0ml @16:27:28.31 Exhaust.select :close @16:27:28.45 SSV.dial CBV @16:27:28.48
CBV.dial SSV @16:27:29.39 Allowing 130s for 25.0ml of remaining 1450.0ml @16:27:29.41 Intake.select :open @16:27:31.61 Intake @15.9psia, vacuum from 9.4 to 13.4psi @16:27:31.64 SS.reconfigure SSinhale.with maxSpeed: 35 @16:27:31.72 SS.seek 25ml @16:27:41.87 Vacuum too high -- waiting up to 40s @16:27:46.97 SS.reconfigure SSinhale.with maxSpeed: 32 @16:27:47.04 SS.seek 25ml @16:27:58.17 Vacuum too high -- waiting up to 40s @16:28:04.29 Intake @16.0psia, vacuum from 9.5 to 13.5psi @16:28:04.32 SS.reconfigure SSinhale.with maxSpeed: 29 @16:28:04.41 SS.seek 25ml @16:28:18.05 Vacuum too high -- waiting up to 40s @16:28:24.93 Intake @16.1psia, vacuum from 9.6 to 13.6psi @16:28:24.98 SS.reconfigure SSinhale.with maxSpeed: 26 @16:28:25.05 SS.seek 25ml @16:28:41.15 Vacuum too high -- waiting up to 40s @16:28:48.77 Intake @16.0psia, vacuum from 9.5 to 13.5psi @16:28:48.79 SS.reconfigure SSinhale.with maxSpeed: 23 @16:28:48.88 SS.seek 25ml @16:29:01.88 Stroke completed -- waiting for pressure to equalize ... @16:29:13.29 Intake.select :close @16:29:13.47 SSV.dial :exhaust @16:29:14.36 Exhaust.select :open @16:29:14.49 SS.reconfigure SSexhale @16:29:14.57 SS.seek 0ml @16:29:24.32 Exhaust.select :close @16:29:24.45 SSV.dial CBV @16:29:24.48
CBV.dial SSV @16:29:25.39 Allowing 130s for 25.0ml of remaining 1425.0ml @16:29:25.41 Intake.select :open @16:29:27.61 Intake @15.8psia, vacuum from 9.3 to 13.3psi @16:29:27.64 SS.reconfigure SSinhale.with maxSpeed: 23 @16:29:27.72 SS.seek 25ml @16:29:48.06 Vacuum too high -- waiting up to 40s @16:29:55.39 Intake @15.8psia, vacuum from 9.3 to 13.3psi @16:29:55.42 SS.reconfigure SSinhale.with maxSpeed: 21 @16:29:55.50 SS.seek 25ml @16:30:13.74 Vacuum too high -- waiting up to 40s @16:30:22.37 Intake @15.9psia, vacuum from 9.4 to 13.4psi @16:30:22.39 SS.reconfigure SSinhale.with maxSpeed: 19 @16:30:22.48 SS.seek 25ml @16:30:45.09 Vacuum too high -- waiting up to 40s @16:30:56.40 Intake @15.7psia, vacuum from 9.2 to 13.2psi @16:30:56.42 SS.reconfigure SSinhale.with maxSpeed: 17 @16:30:56.51 SS.seek 25ml @16:31:20.15 Vacuum too high -- waiting up to 40s @16:31:33.55 SS.reconfigure SSinhale.with maxSpeed: 15 @16:31:33.63 SS.seek 25ml @16:31:35.42 SS.reconfigure SSconfig @16:31:35.50 SS.hold @16:31:35.72 Intake.select :close @16:31:35.89 ShallowSampler::Clogged in MAIN -- progress too slow after 98.7ml @16:31:35.91 SSV.dial :exhaust @16:31:36.81 Exhaust.select :open @16:31:36.95 SS.reconfigure SSexhale @16:31:37.02 SS.seek 0ml @16:31:46.30 Exhaust.select :close @16:31:46.44 Sampled 98.7ml @16:31:46.46 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @16:31:46.48 Intake.select :close @16:31:46.58 CSV.dial :waste @16:31:46.66 CS.seek 0ml @16:31:46.80 SSV.dial CBV,avoiding: :exhaust @16:31:46.83 CSV.dial :air @16:31:46.87
CBV.dial SSV @16:31:47.84 CS.seek 10ml @16:31:49.13
CTV.dial :air @16:31:50.07 delay 15 seconds @16:32:05.10 SSV.dial :spit,avoiding: :exhaust @16:32:06.70 CTV.dial CSV,avoiding: :intake @16:32:06.74
SS.seek 0ml @16:32:06.90 SSV.dial CBV,avoiding: :exhaust @16:32:15.88 CBV.dial between SSV and waste2 @16:32:16.34 CTV.dial CSV,avoiding: :air @16:32:16.37
CSV.dial CTV @16:32:16.93 CS.reconfigure CSpressurize @16:32:17.01 CS.seek 0ml @16:32:44.04 CSV.dial :air @16:32:44.60 CS.reconfigure CSfast @16:32:44.68 CS.seek 10ml @16:33:12.03 CSV.dial between air and CTV @16:33:12.44 CS.seek 2.526ml @16:33:33.13 CTV.dial CSV,avoiding: :air @16:33:33.17
CSV.dial CTV @16:33:33.55 CS.reconfigure CSpressurize @16:33:33.63 CS.seek 0ml @16:33:37.46 BubblePoint: Intake @70.3psia, Outlet @14.9psia @16:33:37.47 CBV.dial SSV @16:33:37.88 SS.reconfigure SSconfig @16:33:37.96 SS.seek 10ml @16:33:43.74 delay 15 seconds @16:33:58.87 Evacuated: Intake @38.2psia, Outlet @12.2psia @16:33:58.89 SSV.dial :spit,avoiding: :exhaust @16:34:00.45 SS.reconfigure SSexhale @16:34:00.53 SS.seek 0ml @16:34:05.67 SS.reconfigure SSfast @16:34:05.74 SS.seek 5ml @16:34:08.81 SSV.dial CBV,avoiding: :exhaust @16:34:08.85
CBV.dial SSV @16:34:10.88 CTV.dial :air,avoiding: :intake @16:34:12.52 CSV.dial CTV @16:34:12.59
SS.seek 10ml @16:34:13.57 CSV.dial :air @16:34:14.13 CS.reconfigure CSfast @16:34:14.20 CS.seek 0ml @16:34:15.27
SSV.dial :spit,avoiding: :exhaust @16:34:16.86 SS.reconfigure SSexhale @16:34:16.93 SS.seek 0ml @16:34:18.79 CS.coast @16:34:22.06
SS.reconfigure SSfast @16:34:22.15 SS.seek 5ml @16:34:25.02 SSV.dial CBV,avoiding: :exhaust @16:34:25.06
CBV.dial SSV @16:34:27.64 SSV.dial :spit,avoiding: :exhaust @16:34:29.22 SS.reconfigure SSconfig @16:34:29.29 SS.seek 0ml @16:34:32.55 SS.coast @16:34:32.62 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @16:34:32.63 Intake.select :close @16:34:32.76 CSV.dial :waste @16:34:32.91 SSV.dial CBV,avoiding: :exhaust @16:34:32.93
CBV.dial SSV @16:34:33.75 CS.seek 0ml @16:34:33.84 CSV.dial :air @16:34:34.55
CTV.dial :air @16:34:34.57 delay 15 seconds @16:34:34.78 CS.seek 10ml @16:34:49.61
SSV.dial :spit,avoiding: :exhaust @16:34:51.20 CTV.dial CSV,avoiding: :intake @16:34:51.27
SS.reconfigure SSexhale @16:34:51.36 SS.seek 0ml @16:34:51.45 SSV.dial CBV,avoiding: :exhaust @16:35:02.63 CBV.dial between SSV and waste2 @16:35:03.11 CSV.dial between air and CTV @16:35:03.50 CS.seek 8.238ml @16:35:09.35 CTV.dial CSV,avoiding: :air @16:35:09.39
CSV.dial CTV @16:35:09.78 CS.reconfigure CSpressurize @16:35:09.85 CS.seek 0ml @16:35:32.26 CSV.dial :air @16:35:32.83 CS.reconfigure CSfast @16:35:32.91 CS.seek 10ml @16:36:00.38 CSV.dial between air and CTV @16:36:00.78 CS.seek 3.102ml @16:36:20.04 CTV.dial CSV,avoiding: :air @16:36:20.08
CSV.dial CTV @16:36:20.47 CS.reconfigure CSpressurize @16:36:20.54 CS.seek 0ml @16:36:28.63 BubblePoint: Intake @69.7psia, Outlet @14.6psia @16:36:28.64 CBV.dial SSV @16:36:29.05 SS.reconfigure SSconfig @16:36:29.13 SS.seek 10ml @16:36:34.86 delay 15 seconds @16:36:49.99 Evacuated: Intake @39.6psia, Outlet @16.4psia @16:36:50.01 SSV.dial :spit,avoiding: :exhaust @16:36:51.59 SS.reconfigure SSexhale @16:36:51.67 SS.seek 0ml @16:36:56.79 SS.reconfigure SSfast @16:36:56.86 SS.seek 5ml @16:36:59.69 SSV.dial CBV,avoiding: :exhaust @16:36:59.72
CBV.dial SSV @16:37:01.31 CTV.dial :air,avoiding: :intake @16:37:02.96 CSV.dial CTV @16:37:03.01
SS.seek 10ml @16:37:04.01 CSV.dial :air @16:37:04.57 CS.reconfigure CSfast @16:37:04.64 CS.seek 0ml @16:37:06.03
SSV.dial :spit,avoiding: :exhaust @16:37:06.56 CS.coast @16:37:07.59
SS.reconfigure SSexhale @16:37:07.67 SS.seek 0ml @16:37:13.29 SS.reconfigure SSfast @16:37:13.37 SS.seek 5ml @16:37:16.27 SSV.dial CBV,avoiding: :exhaust @16:37:16.30
CBV.dial SSV @16:37:18.94 SSV.dial :spit,avoiding: :exhaust @16:37:20.53 SS.reconfigure SSconfig @16:37:20.60 SS.seek 0ml @16:37:23.79 SS.coast @16:37:23.86 Lysing: Adding 1.5ml lysis @16:37:23.88 CBV.dial between CSV and waste1,avoiding: :DBV @16:37:25.73 CTV.dial CSV @16:37:27.37 CSV.dial CSR @16:37:28.33 Pulling 1ml of CSR.lysis @16:37:28.34 CSR.select :lysis @16:37:28.50 CS.reconfigure CSconfig @16:37:28.56 CS.seek 1ml @16:37:32.20 delay 5 seconds @16:37:37.23 CSR.select :air @16:37:37.42 CS.seek 6ml @16:37:51.66 delay 2 seconds @16:37:53.68 CSV.dial :waste1 @16:37:55.32 CS.reconfigure CSfast @16:37:55.39 CS.seek :empty @16:38:12.07 CSV.dial CSR @16:38:13.76 Pulling 1.5ml of CSR.lysis @16:38:13.77 CSR.select :lysis @16:38:13.93 CS.reconfigure CSconfig @16:38:14.00 CS.seek 1.5ml @16:38:19.04 delay 5 seconds @16:38:24.07 CSR.select :air @16:38:24.27 CS.seek 4.5ml @16:38:33.04 delay 2 seconds @16:38:35.06 CSV.dial CTV @16:38:35.61 CS.seek 1.275ml @16:38:45.08 Heating sh1 puck from tube 2 in Collection Clamp to 85C for 5:00 @16:38:45.10 CH.seek 85C,8:00 @16:42:16.44 CH.hold 5:00,85C @16:47:16.62 Collection Puck Heater at 85C after lysing @16:47:16.65 CS.seek 0ml @16:47:21.16 CTV.dial between spare and air,avoiding: :intake @16:47:22.46 CSV.dial :air @16:47:23.04 CS.seek 5ml @16:47:37.30 CSV.dial CTV @16:47:37.86 delay 2 seconds @16:47:39.88 CSV.dial :air @16:47:40.45 delay 2 seconds @16:47:42.47 CSV.dial CTV @16:47:43.06 delay 2 seconds @16:47:45.08 CSV.dial :air @16:47:45.65 delay 2 seconds @16:47:47.68 CS.seek 0ml @16:48:01.83 Recovering Lysate into Collection syringe @16:48:01.84 CSV.dial CBV @16:48:03.14 CBV.dial CSV @16:48:03.60 CS.seek 5ml @16:48:17.80 delay 5 seconds @16:48:22.83 CTV.dial :air @16:48:23.46 CS.seek 7ml @16:48:29.76 delay 5 seconds @16:48:34.79 CSV.dial :air2 @16:48:35.35 CS.seek 10ml @16:48:44.30 CSV.dial between air and CTV,avoiding: CBV @16:48:45.73 Lysing Complete @16:48:45.83 Storing sh1 puck in Collection Clamp to tube 1 @16:48:46.04 SC.reconfigure SCtight @16:48:46.11 SC.seek 1 @16:48:48.25 SC.reconfigure SCloose @16:48:48.50 Elbow.reconfigure TightElbow @16:48:48.57 Elbow.seek 1 @16:48:50.58 Forearm.reconfigure TightForearm @16:48:50.65 Forearm.seek 1 + 700 counts @16:48:51.73 SE.reconfigure SEpick @16:48:51.84 SE.seek beamBreak @16:48:54.69 SE.configure SEconfig @16:48:54.89 Elbow.reconfigure LimpElbow @16:48:54.97 Forearm.reconfigure LooseForearm @16:48:55.04 Forearm.seek 1 @16:48:55.36 Elbow.hold @16:48:55.47 Elbow.reconfigure TightElbow @16:48:55.59 SE.seek 5.09 Pucks below Top Plate @16:48:56.09 Forearm.force -20 @16:48:56.15 Forearm.seek 1 + 340 counts @16:48:56.93 Forearm.reconfigure TightForearm @16:48:57.07 Forearm.seek 1 + 700 counts @16:48:57.37 SE.seek 6.09 Pucks below Top Plate @16:48:58.14 Forearm.reconfigure LooseForearm @16:48:58.21 Forearm.seek :retract @16:48:59.16 Elbow.seek CC @16:49:00.77 Elbow.reconfigure LooseElbow @16:49:00.89 Forearm.seek CC @16:49:02.00 Hand.close @16:49:02.77 CC.seek :open @16:49:07.34 Forearm.seek :retract @16:49:08.42 Elbow.reconfigure TightElbow @16:49:08.49 Elbow.seek 1 @16:49:10.46 Elbow.reconfigure LimpElbow @16:49:10.54 Forearm.seek 1 @16:49:11.61 Elbow.hold @16:49:11.71 Elbow.reconfigure TightElbow @16:49:11.78 Hand.open @16:49:12.66 Forearm.force -20 @16:49:12.73 Forearm.seek 1 + 334 counts @16:49:13.08 Forearm.reconfigure TightForearm @16:49:13.15 Forearm.seek 1 + 700 counts @16:49:13.49 SE.seek :down @16:49:14.64
Forearm.reconfigure LooseForearm @16:49:14.71 Forearm.seek :retract @16:49:15.73 SE.coast @16:49:15.79
SC.coast @16:49:15.84 Tranferring lysate to PRV @16:49:15.85 CSV.dial :air @16:49:16.23 CS.seek 10ml @16:49:16.32 CSV.dial between air and CTV @16:49:16.70 AIV.dial :bypass @16:49:16.81 Beginning samplePassC @16:49:16.83 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:49:16.93 Hand.open @16:49:17.27 Elbow.seek FlushPuck::Garage @16:49:19.30 Elbow.reconfigure LooseElbow @16:49:19.43 Forearm.seek :garage @16:49:20.46 Hand.close @16:49:21.40 Forearm.seek :retract @16:49:22.44 Elbow.reconfigure TightElbow @16:49:22.51 Elbow.seek CC @16:49:25.34 Elbow.reconfigure LooseElbow @16:49:25.48 Forearm.reconfigure TightForearm @16:49:25.55 Forearm.seek CC @16:49:27.42 CC.seek :closed @16:49:31.71 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.51 @16:49:31.83 Hand.open @16:49:32.71 Forearm.reconfigure LooseForearm @16:49:32.78 Forearm.seek :retract @16:49:33.81 AWV.select :air @16:49:33.82 AIV.dial :bypass @16:49:33.85 PSV.dial PSR @16:49:35.48 Pulling 1.5ml of PSR.flush @16:49:35.49 PSR.select :flush @16:49:35.65 PS.seek 1.5ml @16:49:52.22 delay 5 seconds @16:49:57.25 PSV.dial :waste1 @16:49:57.90 PS.seek 0ml @16:50:14.28 PSV.dial PSR @16:50:14.81 PSR.select :air @16:50:14.95 PS.seek 2.5ml @16:50:41.44 PRV.dial CBV @16:50:41.48
CBV.dial PRV @16:50:42.44 PSV.dial :mixloop @16:50:43.61 CTV.dial :air,avoiding: :intake @16:50:43.62 PTV.dial :PRVmixing @16:50:43.73 PS.seek 0ml @16:51:10.44 PSV.dial :air @16:51:10.98 PS.seek 2.5ml @16:51:37.62 PSV.dial :mixloop @16:51:38.15 PS.seek 0ml @16:52:05.00 PSV.dial :air @16:52:05.54 PS.seek 2.5ml @16:52:32.25 PSV.dial :mixloop @16:52:32.79 PS.seek 0ml @16:52:59.34 Garaging FlushPuck in Collection Clamp @16:52:59.44 Elbow.reconfigure TightElbow @16:52:59.51 Elbow.seek CC @16:52:59.74 Elbow.reconfigure LooseElbow @16:52:59.87 Forearm.seek CC @16:53:00.94 Hand.close @16:53:01.65 CC.seek :open @16:53:06.08 Forearm.seek :retract @16:53:07.16 Elbow.reconfigure TightElbow @16:53:07.23 Elbow.seek :garage @16:53:10.52 Elbow.reconfigure LooseElbow @16:53:10.65 Forearm.reconfigure TightForearm @16:53:10.72 Forearm.seek :garage @16:53:11.87 Hand.open @16:53:12.72 Forearm.reconfigure LooseForearm @16:53:12.79 Forearm.seek :retract @16:53:13.88 Connecting PS to CS to transfer sample @16:53:13.89 CBV.dial AIV @16:53:13.92 AIV.dial :bypass @16:53:13.95 PSV.dial :mixloop @16:53:13.98 PTV.dial :PRVmixing @16:53:14.00 PRV.dial AIV @16:53:14.03 CTV.dial :air,avoiding: :intake @16:53:14.06 Loading lyfil puck from tube 2 into Collection Clamp @16:53:14.35 SC.reconfigure SCtight @16:53:14.42 SC.seek 2 @16:53:17.09 SC.reconfigure SCloose @16:53:17.34 Elbow.reconfigure TightElbow @16:53:17.41 Elbow.seek 2 @16:53:19.66 Forearm.reconfigure TightForearm @16:53:19.73 Forearm.seek 2 + 700 counts @16:53:20.80 SE.reconfigure SEpick @16:53:20.90 SE.seek beamBreak @16:53:24.06 SE.configure SEconfig @16:53:24.25 Elbow.seek 2 @16:53:24.48 Forearm.seek 2 + 300 counts @16:53:24.73 Forearm.reconfigure LooseForearm @16:53:24.79 Hand.close @16:53:25.54 SE.seek :down @16:53:25.78
Forearm.seek :retract @16:53:26.77 Elbow.seek CC @16:53:28.45 SE.coast @16:53:28.47
Elbow.reconfigure LooseElbow @16:53:28.64 Forearm.reconfigure TightForearm @16:53:28.71 Forearm.seek CC @16:53:30.09 CC.seek :closed @16:53:34.40 Collection Clamp onSpring/offSpring for 7/30 samples, on/off torque 2.67 @16:53:34.42 Hand.open @16:53:35.22 Forearm.reconfigure LooseForearm @16:53:35.29 Forearm.seek :retract @16:53:36.37 SC.coast @16:53:36.42 CTV.dial CSV,avoiding: :intake @16:53:38.08 CSV.dial CTV @16:53:38.47 CS.seek 5ml @16:53:52.67 delay 1:20 @16:55:12.71 CS.seek 2.5ml @16:55:20.36 delay 40 seconds @16:56:00.39 CS.seek 0ml @16:56:08.08 delay 1:00 @16:57:08.11 PSV.dial :air @16:57:08.64 PS.seek 2.5ml @16:57:35.27 PSV.dial :mixloop @16:57:35.80 delay 3 seconds @16:57:38.82 PSV.dial :air @16:57:39.33 delay 2 seconds @16:57:41.35 PSV.dial :mixloop @16:57:41.86 delay 3 seconds @16:57:44.89 PSV.dial :waste1 @16:57:46.40 PS.seek :empty @16:58:13.22 CBV.dial :waste1 @16:58:13.79 delay 5 seconds @16:58:18.82 CBV.dial AIV @16:58:19.40 delay 10 seconds @16:58:29.43 CBV.dial :waste1 @16:58:30.00 delay 10 seconds @16:58:40.03 CBV.dial AIV @16:58:40.60 delay 10 seconds @16:58:50.63 CTV.dial CSV,avoiding: :intake @16:58:50.64 CBV.dial between AIV and waste1 @16:58:51.07 CSV.dial :air @16:58:51.66 CS.seek 10ml @16:59:18.98 CSV.dial CTV @16:59:19.56 CS.seek 0ml @16:59:47.76 CBV.dial :waste1 @16:59:48.13 delay 10 seconds @16:59:58.16 CTV.dial :air,avoiding: :intake @16:59:59.89 Storing lyfil puck in Collection Clamp to tube 1 @17:00:00.10 SC.reconfigure SCtight @17:00:00.17 SC.seek 1 @17:00:02.29 SC.reconfigure SCloose @17:00:02.53 Elbow.reconfigure TightElbow @17:00:02.60 Elbow.seek 1 @17:00:04.69 Forearm.reconfigure TightForearm @17:00:04.76 Forearm.seek 1 + 700 counts @17:00:05.82 SE.reconfigure SEpick @17:00:05.92 SE.seek beamBreak @17:00:08.65 SE.configure SEconfig @17:00:08.84 Elbow.reconfigure LimpElbow @17:00:08.91 Forearm.reconfigure LooseForearm @17:00:08.99 Forearm.seek 1 @17:00:09.33 Elbow.hold @17:00:09.42 Elbow.reconfigure TightElbow @17:00:09.55 SE.seek 6.08 Pucks below Top Plate @17:00:10.06 Forearm.force -20 @17:00:10.13 Forearm.seek 1 + 340 counts @17:00:10.46 Forearm.reconfigure TightForearm @17:00:10.53 Forearm.seek 1 + 700 counts @17:00:10.82 SE.seek 7.08 Pucks below Top Plate @17:00:11.58 Forearm.reconfigure LooseForearm @17:00:11.65 Forearm.seek :retract @17:00:12.62 Elbow.seek CC @17:00:14.25 Elbow.reconfigure LooseElbow @17:00:14.38 Forearm.seek CC @17:00:15.47 Hand.close @17:00:16.28 CC.seek :open @17:00:20.83 Forearm.seek :retract @17:00:21.92 Elbow.reconfigure TightElbow @17:00:21.99 Elbow.seek 1 @17:00:24.04 Elbow.reconfigure LimpElbow @17:00:24.11 Forearm.seek 1 @17:00:25.16 Elbow.hold @17:00:25.26 Elbow.reconfigure TightElbow @17:00:25.33 Hand.open @17:00:26.30 Forearm.force -20 @17:00:26.36 Forearm.seek 1 + 350 counts @17:00:26.69 Forearm.reconfigure TightForearm @17:00:26.76 Forearm.seek 1 + 700 counts @17:00:27.16 SE.seek :down @17:00:28.30
Forearm.reconfigure LooseForearm @17:00:28.37 Forearm.seek :retract @17:00:29.36 SE.coast @17:00:29.40
SC.coast @17:00:29.46 PSV.dial :air @17:00:31.23 PS.seek 0.499ml @17:00:37.22 delay 3 seconds @17:00:40.24 PSV.dial :mixloop @17:00:40.74 PRV.dial :air @17:00:41.27 delay 3 seconds @17:00:44.30 PRV.dial CBV @17:00:44.83 CBV.dial AIV @17:00:45.38 PRV.dial between CBV and air @17:00:45.75 PRV.dial :air @17:00:46.18 PS.reconfigure PSslow @17:00:46.25 PS.seek 0.599ml @17:00:48.75 PRV.dial :waste2 @17:00:49.58 PS.reconfigure PSconfig @17:00:49.65 PS.seek :empty @17:00:56.69 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:00:56.78 Hand.open @17:00:57.16 Elbow.seek FlushPuck::Garage @17:00:59.15 Elbow.reconfigure LooseElbow @17:00:59.28 Forearm.seek :garage @17:01:00.34 Hand.close @17:01:01.29 Forearm.seek :retract @17:01:02.39 Elbow.reconfigure TightElbow @17:01:02.46 Elbow.seek CC @17:01:05.39 Elbow.reconfigure LooseElbow @17:01:05.52 Forearm.reconfigure TightForearm @17:01:05.59 Forearm.seek CC @17:01:06.96 CC.seek :closed @17:01:11.24 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.40 @17:01:11.33 Hand.open @17:01:12.23 Forearm.reconfigure LooseForearm @17:01:12.30 Forearm.seek :retract @17:01:13.43 Clearing PML for modified lysate @17:01:13.44 CTV.dial :air,avoiding: :intake @17:01:13.47 PRV.dial :waste2 @17:01:13.51 PTV.dial :PRV,avoiding: :puck @17:01:14.38 delay 2 seconds @17:01:16.40 PTV.dial :PRVmixing,avoiding: :puck @17:01:17.25 delay 2 seconds @17:01:19.27 PSV.dial :PML @17:01:19.29 delay 2 seconds @17:01:21.33 PRV.dial :air @17:01:22.19 delay 2 seconds @17:01:24.27 PS.seek 1.499ml @17:01:40.56 delay 5 seconds @17:01:45.60 PS.seek 2.5ml @17:01:56.85 PSV.dial :air @17:01:57.39 PS.seek 0ml @17:02:24.00 mixing lysate with speDil @17:02:24.01 PSV.dial :PML @17:02:24.55 CBV.dial :PRV @17:02:24.57 PRV.dial :air @17:02:24.60 delay 2 seconds @17:02:26.64 PRV.dial :speDil @17:02:27.17 delay 2 seconds @17:02:29.26 PS.reconfigure PSslow1 @17:02:29.33 PS.seek 0.225ml @17:02:53.32 delay 5 seconds @17:02:58.86 PRV.dial CBV @17:02:59.75 delay 2 seconds @17:03:01.84 PS.seek 0.475ml @17:03:27.60 delay 5 seconds @17:03:32.63 PRV.dial :air @17:03:33.17 delay 2 seconds @17:03:35.21 PS.seek 0.965ml @17:04:25.28 delay 10 seconds @17:04:35.31 PRV.dial CBV @17:04:35.85 CTV.dial :air,avoiding: :intake @17:04:35.87 PSV.dial :air @17:04:36.39 PS.reconfigure PSconfig @17:04:36.47 PS.seek 2.5ml @17:04:53.13 delay 3 seconds @17:04:56.15 PSV.dial PTV @17:04:57.00 PTV.dial :PRV,avoiding: :puck @17:04:57.86 PS.seek 0ml @17:05:24.57 PSV.dial :air @17:05:25.43 PS.seek 2.5ml @17:05:51.97 delay 3 seconds @17:05:54.99 PSV.dial PTV @17:05:55.85 PTV.dial :PRV,avoiding: :puck @17:05:55.88 PS.seek 0ml @17:06:22.54 Pushing modified lysate into AIV Injection Loop @17:06:22.55 CBV.dial AIV @17:06:22.59 AIV.dial :bypass @17:06:22.61 PRV.dial CBV @17:06:22.64 PSV.dial :air @17:06:23.53 PS.seek 2.5ml @17:06:50.25 delay 3 seconds @17:06:53.27 PSV.dial :PML @17:06:53.79 PTV.dial :PRVmixing,avoiding: :puck @17:06:54.73 PS.reconfigure PSslow1 @17:06:54.80 PS.seek 1.74ml @17:08:12.25 delay 5 seconds @17:08:17.28 AIV.dial :sample @17:08:17.98 AWV.select :waste @17:08:18.17 PS.reconfigure PSslow2 @17:08:18.24 PS.seek 1.22ml @17:10:05.63 delay 15 seconds @17:10:20.66 AIV.dial :bypass @17:10:21.34 PS.reconfigure PSconfig @17:10:21.42 PS.seek 0ml @17:10:34.79 PSV.dial :air @17:10:35.33 PS.seek 2.5ml @17:11:01.63 PSV.dial :PML @17:11:02.17 PS.seek 0ml @17:11:28.66 AIV.dial :coilToMFB @17:11:28.67 AWV.select :air @17:11:28.87 Extracting Nucleic Acid from AIV @17:11:28.90
Beginning samplePassD @17:11:28.94 SPE.hold 2:46:40,55C @17:11:29.04 ASV.dial :SPEdelivery @17:11:29.17
AWV.select :air @17:11:29.18 AIV.dial :bypass @17:11:29.21 PSV.dial PSR @17:11:29.52 delay 20 seconds @17:11:30.57
Pulling 1.5ml of PSR.killMFB @17:11:30.59 PSR.select :killMFB @17:11:30.75 PS.seek 1.5ml @17:11:48.04 delay 5 seconds @17:11:49.55 primeLysateINT @17:11:49.56 AWV.select :air @17:11:49.59 AIV.dial :coilToMFB @17:11:49.62 ATV.dial AIV @17:11:51.62 AS.reconfigure ASslow1 @17:11:51.69 AS.seek 0.299ml @17:11:53.07
PSV.dial :waste1 @17:11:53.62 PS.seek 0ml @17:12:10.91 PSV.dial PSR @17:12:11.47 PSR.select :air @17:12:11.62 PS.seek 2.5ml @17:12:39.91 PRV.dial CBV @17:12:39.95
CBV.dial PRV @17:12:39.97 PSV.dial :mixloop @17:12:41.22 CTV.dial :air,avoiding: :intake @17:12:41.23 PTV.dial :PRVmixing @17:12:41.79 PS.seek 0ml @17:12:52.36 delay 10 seconds @17:13:02.39 ATV.dial :air2 @17:13:03.34 AS.seek 0.329ml @17:13:09.70 ATV.dial :waste2 @17:13:10.13
PSV.dial PSR @17:13:10.36 AS.reconfigure ASnorm @17:13:10.43 AS.seek :empty @17:13:11.46
Pulling 1.5ml of PSR.flush @17:13:11.47 PSR.select :flush @17:13:11.64 PS.seek 1.5ml @17:13:27.47 Lysate primed! @17:13:27.48 AFV.select :holdingCoil @17:13:27.51 ATV.dial :air2 @17:13:28.23 AS.reconfigure ASslow1 @17:13:28.30 AS.seek 0.069ml @17:13:29.01
delay 5 seconds @17:13:34.04 PSV.dial :waste1 @17:13:34.59 PS.seek 0ml @17:13:42.69 delay 3 seconds @17:13:45.72 ATV.dial :AIV @17:13:46.66 AS.seek 0.469ml @17:13:52.07
PSV.dial PSR @17:13:52.63 PSR.select :air @17:13:52.78 PS.seek 2.5ml @17:14:21.07 PRV.dial CBV @17:14:21.10
CBV.dial PRV @17:14:21.13 PSV.dial :mixloop @17:14:22.40 CTV.dial :air,avoiding: :intake @17:14:22.42 PTV.dial :PRVmixing @17:14:22.46 PS.seek 0ml @17:14:50.44 PSV.dial PSR @17:14:51.74 Pulling 1.5ml of PSR.flush @17:14:51.75 PSR.select :flush @17:14:51.92 PS.seek 1.5ml @17:15:07.38 delay 5 seconds @17:15:08.88
delay 5 seconds @17:15:12.47 Solid Phase Extraction Heater at 49.3C @17:15:12.48 ATV.dial ASV @17:15:12.92 ASV.dial :SPEdelivery @17:15:13.02 AS.seek 0.041ml @17:15:13.91
PSV.dial :waste1 @17:15:14.47 PS.seek 0ml @17:15:31.23 PSV.dial PSR @17:15:31.77 PSR.select :air @17:15:31.92 PS.seek 2.5ml @17:15:59.44 PRV.dial CBV @17:15:59.48
CBV.dial PRV @17:15:59.50 PSV.dial :mixloop @17:16:00.73 CTV.dial :air,avoiding: :intake @17:16:00.75 PTV.dial :PRVmixing @17:16:00.79 PS.seek 0ml @17:16:28.26 PSV.dial PSR @17:16:29.52 Pulling 1.5ml of PSR.flush @17:16:29.54 PSR.select :flush @17:16:29.70 PS.seek 1.5ml @17:16:39.38 delay 10 seconds @17:16:46.29
delay 5 seconds @17:16:49.41 rinseLysateLineINT @17:16:49.42 AFV.select :carrierSolution @17:16:49.56 delay 2 seconds @17:16:51.32
PSV.dial :waste1 @17:16:51.58 AS.reconfigure ASconfig @17:16:51.65 AS.seek 1ml @17:16:51.88
PS.seek 0ml @17:17:08.54 PSV.dial PSR @17:17:09.08 PSR.select :air @17:17:09.23 PS.seek 2.5ml @17:17:36.32 PRV.dial CBV @17:17:36.36
CBV.dial PRV @17:17:36.38 PSV.dial :mixloop @17:17:37.58 CTV.dial :air,avoiding: :intake @17:17:37.59 PTV.dial :PRVmixing @17:17:37.64 PS.reconfigure PSslow @17:17:37.72 PS.seek 1.2ml @17:17:47.06 delay 1 second @17:17:48.08 AFV.select :holdingCoil @17:17:48.19 delay 1 second @17:17:49.22 ATV.dial AIV @17:17:49.80 AIV.dial :coilToMFB @17:17:49.82 AWV.select :waste @17:17:50.48 AS.reconfigure ASslow0 @17:17:50.56 AS.seek 0ml @17:18:04.57
PSV.dial :waste1 @17:18:06.10 PS.reconfigure PSconfig @17:18:06.18 PS.seek 0ml @17:18:19.66 PSV.dial :air @17:18:20.88 PS.seek 2.5ml @17:18:47.60 PSV.dial :mixloop @17:18:48.13 PS.reconfigure PSslow @17:18:48.20 PS.seek 0ml @17:19:31.49 ATV.dial :air2 @17:19:32.21 AS.reconfigure ASslow1 @17:19:32.28 AS.seek 0.05ml @17:19:39.51
PSV.dial :air @17:19:40.04 PS.reconfigure PSconfig @17:19:40.11 PS.seek 2.5ml @17:19:42.67 ATV.dial :AIV @17:19:43.54 AS.seek 0.01ml @17:19:51.86 ATV.dial :waste @17:19:53.58 AS.seek :empty @17:19:56.06 ethanol wash @17:19:56.07 ATV.dial :air2 @17:19:57.42 AS.reconfigure ASslow2 @17:19:57.49 AS.seek 0.009ml @17:20:07.07
PSV.dial :mixloop @17:20:07.60 PS.reconfigure PSslow @17:20:07.68 PS.seek 0ml @17:20:07.94 delay 3 seconds @17:20:10.96 Pulling 0.4ml of Ethanol Wash @17:20:10.97 ATV.dial :ethanol @17:20:11.85 AS.reconfigure ASslow1 @17:20:11.92 AS.seek 0.409ml @17:20:58.85
Cleaning Collection Syringe @17:20:58.86 CSV.dial CSR @17:20:59.48 Pulling 4ml of CSR.flush @17:20:59.50 CSR.select :flush @17:20:59.66 CS.seek 4ml @17:21:11.16 delay 5 seconds @17:21:16.19 CSR.select :air @17:21:16.34 CS.seek 10ml @17:21:32.74 delay 3 seconds @17:21:32.94 CSV.dial CTV @17:21:32.97
CTV.dial CSV @17:21:34.67 CBV.dial :waste1 @17:21:35.27 CS.seek 0ml @17:21:35.76 ATV.dial :air1 @17:21:36.17 delay 3 seconds @17:21:39.26 AS.seek 0.434ml @17:21:44.52 delay 3 seconds @17:21:47.55 ATV.dial :ethanol @17:21:48.20 AS.seek 0.419ml @17:21:51.53 delay 3 seconds @17:21:54.56 ATV.dial ASV @17:21:55.95 AS.seek 0.092ml @17:22:02.44
CSV.dial CSR @17:22:03.06 Pulling 4ml of CSR.flush @17:22:03.07 CSR.select :flush @17:22:03.23 CS.seek 4ml @17:22:14.69 delay 5 seconds @17:22:19.72 CSR.select :air @17:22:19.87 CS.seek 10ml @17:22:36.60 CSV.dial CTV @17:22:36.64
CTV.dial CSV @17:22:37.19 CBV.dial :waste1 @17:22:37.22 CS.seek 0ml @17:23:02.14 delay 3 seconds @17:23:04.57
Garaging FlushPuck in Collection Clamp @17:23:04.67 Elbow.reconfigure TightElbow @17:23:04.74 Elbow.seek CC @17:23:05.04 Elbow.reconfigure LooseElbow @17:23:05.17 Forearm.seek CC @17:23:05.24 ATV.dial :waste2 @17:23:06.05 AS.reconfigure ASconfig @17:23:06.11 AS.seek :empty @17:23:06.75
Hand.close @17:23:07.57 CC.seek :open @17:23:11.98 Forearm.seek :retract @17:23:12.34 ATV.dial :air2 @17:23:12.76 AS.reconfigure ASslow1 @17:23:12.84 AS.seek 1ml @17:23:13.06
Elbow.reconfigure TightElbow @17:23:13.13 Elbow.seek :garage @17:23:16.30 Elbow.reconfigure LooseElbow @17:23:16.43 Forearm.reconfigure TightForearm @17:23:16.50 Forearm.seek :garage @17:23:17.67 Hand.open @17:23:18.51 Forearm.reconfigure LooseForearm @17:23:18.58 Forearm.seek :retract @17:23:19.65 Whole Cell Archive sampling 1500ml @17:23:19.67 Loading wcr puck from tube 2 into Collection Clamp @17:23:19.96 SC.reconfigure SCtight @17:23:20.04 SC.seek 2 @17:23:22.22 SC.reconfigure SCloose @17:23:22.48 Elbow.reconfigure TightElbow @17:23:22.56 Elbow.seek 2 @17:23:24.60 Forearm.reconfigure TightForearm @17:23:24.68 Forearm.seek 2 + 700 counts @17:23:25.78 SE.reconfigure SEpick @17:23:25.89 SE.seek beamBreak @17:23:29.14 SE.configure SEconfig @17:23:29.33 Elbow.seek 2 @17:23:29.57 Forearm.seek 2 + 300 counts @17:23:29.81 Forearm.reconfigure LooseForearm @17:23:29.88 Hand.close @17:23:30.70 SE.seek :down @17:23:30.95 Forearm.seek :retract @17:23:31.95 Elbow.seek CC @17:23:33.67 Elbow.reconfigure LooseElbow @17:23:33.77 SE.coast @17:23:33.89 Forearm.reconfigure TightForearm @17:23:33.96 Forearm.seek CC @17:23:35.32 CC.seek :closed @17:23:39.65 Collection Clamp onSpring/offSpring for 7/30 samples, on/off torque 2.61 @17:23:39.66 Hand.open @17:23:40.51 Forearm.reconfigure LooseForearm @17:23:41.13 Forearm.seek :retract @17:23:42.17 SC.coast @17:23:42.23 collectSample 1500ml w/bubble point of 55psi @17:23:42.25 Priming 7ml @17:23:42.27 Intake.select :close @17:23:42.31 Exhaust.select :close @17:23:42.34 SS.hold @17:23:42.60 Calibrating pressure sensors @17:23:42.61 CTV.dial :air @17:23:44.25 SSV.dial :spit,avoiding: :exhaust @17:23:44.29 SS.reconfigure SSfast @17:23:44.37 SS.seek 5ml @17:23:47.52 SSV.dial CBV @17:23:47.56 CBV.dial SSV @17:23:49.25 delay 3.8 seconds @17:23:53.20 SSV.dial :spit @17:23:54.78 SS.seek 0ml @17:23:57.36 SSV.dial CBV @17:23:58.94 CTV.dial :intake @17:23:59.86 SSV.dial CBV @17:23:59.89 CBV.dial SSV @17:23:59.93 Allowing 40s for 7.0ml of remaining 7.0ml @17:23:59.97 Intake.select :open @17:24:02.17 Intake @16.0psia, vacuum from 9.5 to 13.5psi @17:24:02.20 SS.reconfigure SSinhale.with maxSpeed: 150 @17:24:02.28 SS.seek 7ml @17:24:08.42 Stroke completed -- waiting for pressure to equalize ... @17:24:23.04 Intake.select :close @17:24:23.22 SSV.dial :exhaust @17:24:24.11 Exhaust.select :open @17:24:24.24 SS.reconfigure SSexhale @17:24:24.32 SS.seek 0ml @17:24:28.64 Exhaust.select :close @17:24:28.77 Sampling 1500.0ml @17:24:28.79 Intake.select :close @17:24:28.81 Exhaust.select :close @17:24:28.85 SS.reconfigure SSconfig @17:24:28.92 SS.hold @17:24:29.17 CTV.dial :intake @17:24:29.19 SSV.dial CBV @17:24:29.25 CBV.dial SSV @17:24:30.16 Allowing 130s for 25.0ml of remaining 1500.0ml @17:24:30.19 Intake.select :open @17:24:32.39 Intake @15.8psia, vacuum from 9.3 to 13.3psi @17:24:32.42 SS.reconfigure SSinhale.with maxSpeed: 150 @17:24:32.50 SS.seek 25ml @17:24:37.20 Vacuum too high -- waiting up to 40s @17:24:49.81 Intake @16.4psia, vacuum from 9.9 to 13.9psi @17:24:49.84 SS.reconfigure SSinhale.with maxSpeed: 135 @17:24:49.92 SS.seek 25ml @17:24:54.29 Vacuum too high -- waiting up to 40s @17:25:08.09 Intake @16.5psia, vacuum from 10.0 to 14.0psi @17:25:08.12 SS.reconfigure SSinhale.with maxSpeed: 122 @17:25:08.21 SS.seek 25ml @17:25:12.62 Vacuum too high -- waiting up to 40s @17:25:27.59 SS.reconfigure SSinhale.with maxSpeed: 110 @17:25:27.66 SS.seek 25ml @17:25:32.40 Vacuum too high -- waiting up to 40s @17:25:49.45 Intake @16.5psia, vacuum from 10.0 to 14.0psi @17:25:49.48 SS.reconfigure SSinhale.with maxSpeed: 99 @17:25:49.56 SS.seek 25ml @17:25:51.85 Stroke completed -- waiting for pressure to equalize ... @17:26:02.73 Intake.select :close @17:26:02.91 SSV.dial :exhaust @17:26:03.82 Exhaust.select :open @17:26:03.96 SS.reconfigure SSexhale @17:26:04.04 SS.seek 0ml @17:26:13.28 Exhaust.select :close @17:26:13.42 SSV.dial CBV @17:26:13.45 CBV.dial SSV @17:26:14.38 Allowing 130s for 25.0ml of remaining 1475.0ml @17:26:14.41 Intake.select :open @17:26:16.61 Intake @15.9psia, vacuum from 9.4 to 13.4psi @17:26:16.64 SS.reconfigure SSinhale.with maxSpeed: 99 @17:26:16.72 SS.seek 25ml @17:26:21.16 Vacuum too high -- waiting up to 40s @17:26:31.77 Intake @16.2psia, vacuum from 9.7 to 13.7psi @17:26:31.80 SS.reconfigure SSinhale.with maxSpeed: 89 @17:26:31.89 SS.seek 25ml @17:26:34.50 delay 5 seconds @17:26:35.82 Vacuum too high -- waiting up to 40s @17:26:39.52 ATV.dial ASV @17:26:40.57 ASV.dial :SPEdelivery @17:26:40.61 AS.seek 0.02ml @17:26:47.24 Intake @16.3psia, vacuum from 9.8 to 13.8psi @17:26:47.27 SS.reconfigure SSinhale.with maxSpeed: 80 @17:26:47.36 SS.seek 25ml @17:26:51.79 Vacuum too high -- waiting up to 40s @17:27:05.34 SS.reconfigure SSinhale.with maxSpeed: 72 @17:27:05.41 SS.seek 25ml @17:27:10.44 Vacuum too high -- waiting up to 40s @17:27:25.39 SS.reconfigure SSinhale.with maxSpeed: 65 @17:27:25.46 SS.seek 25ml @17:27:30.72 Vacuum too high -- waiting up to 40s @17:27:45.21 Intake @16.2psia, vacuum from 9.7 to 13.7psi @17:27:45.24 SS.reconfigure SSinhale.with maxSpeed: 59 @17:27:45.33 SS.seek 25ml @17:27:51.03 Vacuum too high -- waiting up to 40s @17:28:09.13 Intake @16.2psia, vacuum from 9.7 to 13.7psi @17:28:09.16 SS.reconfigure SSinhale.with maxSpeed: 53 @17:28:09.25 SS.seek 25ml @17:28:10.20 Stroke completed -- waiting for pressure to equalize ... @17:28:19.10 Intake.select :close @17:28:19.28 SSV.dial :exhaust @17:28:20.19 Exhaust.select :open @17:28:20.33 SS.reconfigure SSexhale @17:28:20.40 SS.seek 0ml @17:28:30.17 Exhaust.select :close @17:28:30.33 SSV.dial CBV @17:28:30.36 CBV.dial SSV @17:28:31.28 Allowing 130s for 25.0ml of remaining 1450.0ml @17:28:31.30 Intake.select :open @17:28:33.51 Intake @15.9psia, vacuum from 9.4 to 13.4psi @17:28:33.54 SS.reconfigure SSinhale.with maxSpeed: 53 @17:28:33.62 SS.seek 25ml @17:28:39.15 Vacuum too high -- waiting up to 40s @17:28:49.98 Intake @15.9psia, vacuum from 9.4 to 13.4psi @17:28:50.01 SS.reconfigure SSinhale.with maxSpeed: 48 @17:28:50.10 SS.seek 25ml @17:28:54.76 Vacuum too high -- waiting up to 40s @17:29:06.39 Intake @16.0psia, vacuum from 9.5 to 13.5psi @17:29:06.42 SS.reconfigure SSinhale.with maxSpeed: 43 @17:29:06.51 SS.seek 25ml @17:29:12.44 Vacuum too high -- waiting up to 40s @17:29:24.98 Intake @16.0psia, vacuum from 9.5 to 13.5psi @17:29:25.01 SS.reconfigure SSinhale.with maxSpeed: 39 @17:29:25.09 SS.seek 25ml @17:29:32.40 Vacuum too high -- waiting up to 40s @17:29:47.89 Intake @15.9psia, vacuum from 9.4 to 13.4psi @17:29:47.92 SS.reconfigure SSinhale.with maxSpeed: 35 @17:29:48.01 SS.seek 25ml @17:29:56.12 Vacuum too high -- waiting up to 40s @17:29:57.98 delay 5 seconds @17:30:03.00 ATV.dial :air2 @17:30:04.10 AS.seek 0.52ml @17:30:12.78 SS.reconfigure SSinhale.with maxSpeed: 32 @17:30:12.85 SS.seek 25ml @17:30:23.15 Vacuum too high -- waiting up to 40s @17:30:41.30 SS.reconfigure SSconfig @17:30:41.37 SS.hold @17:30:41.54 Intake.select :close @17:30:41.72 ShallowSampler::Clogged in WCR -- progress too slow after 71.7ml @17:30:41.75 SSV.dial :exhaust @17:30:42.66 Exhaust.select :open @17:30:42.81 SS.reconfigure SSexhale @17:30:42.88 SS.seek 0ml @17:30:51.56 Exhaust.select :close @17:30:51.69 Sampled 71.7ml @17:30:51.72 Drying puck before fixing for 4:00 @17:30:51.74 CBV.dial :waste2b @17:30:52.68 delay 1:00 @17:31:44.88 delay 5 seconds @17:31:49.91 ATV.dial ASV @17:31:50.94 ASV.dial :wasteR @17:31:51.35 AS.seek 0.3ml @17:31:52.71 CSV.dial :air @17:31:53.26 CS.reconfigure CSfast @17:31:53.34 CS.seek 10ml @17:32:20.60 Pumping wcr puck from tube 2 up to 55psi... @17:32:20.62 CBV.dial SSV @17:32:22.14 CBV.dial between CSV and waste2b @17:32:23.34 CSV.dial between air and CTV @17:32:23.75 CS.seek 7.499ml @17:32:31.33 CTV.dial CSV,avoiding: :air @17:32:31.36 CSV.dial CTV @17:32:32.34 CS.reconfigure CSpressurize @17:32:32.42 CS.seek 0ml @17:32:35.89 delay 20 seconds @17:32:49.44 CBV.dial :waste2b @17:32:49.85 CS.reconfigure CSconfig @17:32:49.93 CS.seek :empty @17:32:54.38 delay 1:00 @17:32:55.92 AS.seek 0.07ml @17:33:42.47 delay 20 seconds @17:33:54.41 CTV.dial :air,avoiding: :intake @17:33:56.08 Fix #1 of 2 (with rnal) for 2:00 @17:33:56.09 CSV.dial CSR @17:33:56.71 Pulling 2ml of CSR.rnal @17:33:56.73 CSR.select :rnal @17:33:56.88 CS.seek 2ml @17:34:02.50 ATV.dial :waste2 @17:34:03.28 AS.reconfigure ASconfig @17:34:03.37 delay 5 seconds @17:34:03.39 AS.seek :empty @17:34:07.81 Elution @17:34:07.82 ATV.dial :air1 @17:34:08.40 CSR.select :air @17:34:08.54 CS.reconfigure CSfast @17:34:08.61 CS.seek 10ml @17:34:09.01 AS.reconfigure ASslow1 @17:34:09.08 AS.seek 0.049ml @17:34:19.47 delay 3 seconds @17:34:22.50 ATV.dial :ElutionWater @17:34:23.21 AS.seek 0.109ml @17:34:30.64 Pumping wcr puck from tube 2 up to 55psi... @17:34:30.66 CBV.dial SSV @17:34:32.18 CBV.dial between CSV and waste2b @17:34:33.40 CSV.dial between air and CTV @17:34:34.16 CS.seek 7.231ml @17:34:35.64 delay 3 seconds @17:34:38.68 ATV.dial ASV,via: :air2 @17:34:40.87 Session Begun @17:34:41.41 ASV.dial :SPEelution @17:34:42.05 AFV.select :carrierSolution @17:34:42.25 AS.reconfigure ASnorm @17:34:42.34 AS.seek 0.759ml @17:34:42.55 CTV.dial CSV,avoiding: :air @17:34:42.59 CSV.dial CTV @17:34:44.32 CS.reconfigure CSpressurize @17:34:44.41 CS.seek 0ml @17:34:48.81 -> showlog 100 @17:35:02.91 delay 2:00 @17:35:15.47 delay 3 seconds @17:35:18.50 AFV.select :holdingCoil @17:35:18.69 AS.reconfigure ASslow1 @17:35:18.76 AS.seek 0.474ml @17:36:02.14 -> exit @17:36:02.16 Session Ended @17:36:16.41 delay 5 seconds @17:36:21.51 AS.reconfigure ASslow2 @17:36:21.58 AS.seek 0.439ml @17:36:56.92 delay 1:00 @17:37:02.95 CBV.dial :waste2b @17:37:03.36 CS.reconfigure CSconfig @17:37:03.44 CS.seek :empty @17:37:05.64 delay 1:00 @17:37:56.94 pushing eluate to holding coil @17:37:57.03 AS.seek 0.279ml @17:38:05.67 CTV.dial :air,avoiding: :intake @17:38:07.32 Fix #2 of 2 (with rnal) for 2:00 @17:38:07.34 CSV.dial CSR @17:38:07.96 Pulling 2ml of CSR.rnal @17:38:07.98 CSR.select :rnal @17:38:08.14 CS.seek 2ml @17:38:14.45 delay 5 seconds @17:38:19.48 CSR.select :air @17:38:19.64 CS.reconfigure CSfast @17:38:19.71 CS.seek 10ml @17:38:41.73 Pumping wcr puck from tube 2 up to 55psi... @17:38:41.75 CBV.dial SSV @17:38:43.25 CBV.dial between CSV and waste2b @17:38:44.46 CSV.dial between air and CTV @17:38:45.23 CS.seek 7.231ml @17:38:53.59 CTV.dial CSV,avoiding: :air @17:38:53.62 CSV.dial CTV @17:38:55.33 CS.reconfigure CSpressurize @17:38:55.41 CS.seek 0ml @17:39:14.03 delay 2:00 @17:40:38.08 delay 30 seconds @17:41:08.11 ATV.dial :waste,via: :air3 @17:41:09.88 AS.reconfigure ASconfig @17:41:09.94 AS.seek :empty @17:41:14.06 CBV.dial :waste2b @17:41:14.48 CS.reconfigure CSconfig @17:41:14.56 CS.seek :empty @17:41:16.64 delay 1:00 @17:41:26.34 Parking Bleach in LysateSource line and SPEcolumn @17:41:26.35 ATV.dial :air3 @17:41:27.24 AS.reconfigure ASslow1 @17:41:27.31 AS.seek 0.029ml @17:41:33.74 ATV.dial :ElutionWater @17:41:34.38 AS.seek 0.059ml @17:41:40.80 ATV.dial :air3 @17:41:41.45 AS.seek 0.069ml @17:41:43.75 delay 2 seconds @17:41:45.77 ATV.dial :bleach @17:41:46.26 AS.seek 0.769ml @17:42:16.67 CTV.dial :air,avoiding: :intake @17:42:18.38 Storing wcr puck in Collection Clamp to tube 1 @17:42:19.14 SC.reconfigure SCtight @17:42:19.21 SC.seek 1 @17:42:21.36 SC.reconfigure SCloose @17:42:21.62 Elbow.reconfigure TightElbow @17:42:21.71 Elbow.seek 1 @17:42:23.64 Forearm.reconfigure TightForearm @17:42:23.72 Forearm.seek 1 + 700 counts @17:42:24.81 SE.reconfigure SEpick @17:42:24.92 SE.seek beamBreak @17:42:27.55 SE.configure SEconfig @17:42:27.74 Elbow.reconfigure LimpElbow @17:42:27.82 Forearm.reconfigure LooseForearm @17:42:27.89 Forearm.seek 1 @17:42:28.20 Elbow.hold @17:42:28.30 Elbow.reconfigure TightElbow @17:42:28.42 SE.seek 7.1 Pucks below Top Plate @17:42:28.97 Forearm.force -20 @17:42:29.04 Forearm.seek 1 + 346 counts @17:42:29.38 Forearm.reconfigure TightForearm @17:42:29.46 Forearm.seek 1 + 700 counts @17:42:29.81 SE.seek 8.1 Pucks below Top Plate @17:42:30.58 Forearm.reconfigure LooseForearm @17:42:30.65 Forearm.seek :retract @17:42:31.59 Elbow.seek CC @17:42:33.23 Elbow.reconfigure LooseElbow @17:42:33.36 Forearm.seek CC @17:42:34.44 Hand.close @17:42:35.20 CC.seek :open @17:42:39.73 Forearm.seek :retract @17:42:40.83 Elbow.reconfigure TightElbow @17:42:40.90 Elbow.seek 1 @17:42:42.91 Elbow.reconfigure LimpElbow @17:42:43.00 Forearm.seek 1 @17:42:44.06 Elbow.hold @17:42:44.16 Elbow.reconfigure TightElbow @17:42:44.23 Hand.open @17:42:45.08 Forearm.force -20 @17:42:45.15 Forearm.seek 1 + 350 counts @17:42:45.50 Forearm.reconfigure TightForearm @17:42:45.57 Forearm.seek 1 + 700 counts @17:42:45.97 SE.seek :down @17:42:47.12 Forearm.reconfigure LooseForearm @17:42:47.20 Forearm.seek :retract @17:42:48.66 SE.coast @17:42:48.72 SC.coast @17:42:48.79 Starting finalPurge @17:42:48.81 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:42:48.92 Hand.open @17:42:49.34 Elbow.seek FlushPuck::Garage @17:42:51.49 Elbow.reconfigure LooseElbow @17:42:51.62 Forearm.seek :garage @17:42:52.69 Hand.close @17:42:53.59 Forearm.seek :retract @17:42:54.63 Elbow.reconfigure TightElbow @17:42:54.70 Elbow.seek CC @17:42:57.60 Elbow.reconfigure LooseElbow @17:42:57.72 Forearm.reconfigure TightForearm @17:42:57.79 Forearm.seek CC @17:42:59.20 CC.seek :closed @17:43:03.53 Collection Clamp onSpring/offSpring for 8/31 samples, on/off torque 2.56 @17:43:03.64 Hand.open @17:43:04.52 Forearm.reconfigure LooseForearm @17:43:04.59 Forearm.seek :retract @17:43:05.64 Flushing the Collection Valve::Manifold @17:43:05.80 CSV.dial CSR @17:43:06.40 Pulling 5ml of CSR.flush @17:43:06.41 CSR.select :flush @17:43:06.57 CS.seek 5ml @17:43:20.61 delay 5 seconds @17:43:25.64 CSR.select :air @17:43:25.82 CS.reconfigure CSfast @17:43:25.89 CS.seek 7.5ml @17:43:33.39 CSV.dial :waste @17:43:34.98 CS.reconfigure CSconfig @17:43:35.06 CS.seek 0ml @17:43:55.36 Flushing lower manifold's waste1 port @17:43:55.52 CSV.dial :flush @17:43:56.06 CS.reconfigure CSfast @17:43:56.14 CS.seek 5ml @17:44:07.33 delay 3 seconds @17:44:10.17
delay 5 seconds @17:44:10.35 ATV.dial :air3 @17:44:10.94 delay 3 seconds @17:44:14.03 AS.seek 0.794ml @17:44:15.19
CSV.dial :air @17:44:15.81 CS.seek 7ml @17:44:19.39 delay 3 seconds @17:44:22.05 CSV.dial CTV @17:44:22.09
CTV.dial CSV @17:44:22.41 ATV.dial :bleach @17:44:23.07 AS.seek 0.779ml @17:44:23.80
CBV.dial :waste1 @17:44:24.74 CS.seek 0ml @17:44:26.41 delay 3 seconds @17:44:29.44 ATV.dial :AIV @17:44:30.73 AS.reconfigure ASconfig @17:44:30.80 AS.seek 0.015ml @17:44:44.52
Flushing lower manifold's waste2b port @17:44:44.69 CSV.dial :flush @17:44:45.62 CS.seek 5ml @17:44:59.64 delay 5 seconds @17:45:04.67 CSV.dial :air @17:45:05.32 CS.seek 7ml @17:45:11.52 CSV.dial CTV @17:45:11.56
CTV.dial CSV @17:45:12.11 CBV.dial :waste2b @17:45:13.06 CS.seek 0ml @17:45:15.03 ATV.dial :waste @17:45:16.60 AS.seek :empty @17:45:17.81 ATV.dial :air3 @17:45:18.51 AS.reconfigure ASslow1 @17:45:18.58 AS.seek 0.009ml @17:45:20.89 delay 2 seconds @17:45:22.91 ATV.dial :bleach @17:45:23.40 AS.seek 0.579ml @17:45:32.39
Flushing lower manifold's waste1 port @17:45:32.41 CSV.dial CSR @17:45:33.03 Pulling 4ml of CSR.mfbkill @17:45:33.04 CSR.select :mfbkill @17:45:33.21 CS.reconfigure CSconfig @17:45:33.28 CS.seek 4ml @17:45:44.80 delay 5 seconds @17:45:49.83 CSR.select :air @17:45:49.97 CS.reconfigure CSfast @17:45:50.05 CS.seek 10ml @17:46:06.67 CSV.dial CTV @17:46:06.70
CTV.dial CSV @17:46:07.30 CBV.dial :waste1 @17:46:08.23 CS.seek 0ml @17:46:35.05 Flushing lower manifold's waste2b port @17:46:35.07 CSV.dial CSR @17:46:35.70 Pulling 4ml of CSR.mfbkill @17:46:35.71 CSR.select :mfbkill @17:46:35.88 CS.reconfigure CSconfig @17:46:35.95 CS.seek 4ml @17:46:47.33 delay 5 seconds @17:46:52.36 CSR.select :air @17:46:52.50 CS.reconfigure CSfast @17:46:52.58 CS.seek 10ml @17:47:09.11 CSV.dial CTV @17:47:09.15
CTV.dial CSV @17:47:09.72 CBV.dial :waste2b @17:47:10.67 CS.seek 0ml @17:47:18.36 delay 3 seconds @17:47:21.39 ATV.dial :air3 @17:47:21.97 delay 3 seconds @17:47:25.06 AS.seek 0.604ml @17:47:30.39 delay 3 seconds @17:47:33.42 ATV.dial :bleach @17:47:34.07 AS.seek 0.589ml @17:47:37.41 delay 3 seconds @17:47:37.64
Killing upper manifold @17:47:37.65 CSV.dial CSR @17:47:38.30 Pulling 4ml of CSR.mfbkill @17:47:38.32 CSR.select :mfbkill @17:47:38.47 CS.reconfigure CSconfig @17:47:38.54 CS.seek 4ml @17:47:40.43 ATV.dial :ASV @17:47:41.50 ASV.dial :wasteR @17:47:42.14 AS.seek 0ml @17:47:49.96
delay 5 seconds @17:47:54.99 CSR.select :air @17:47:55.13 CS.reconfigure CSfast @17:47:55.20 CS.seek 10ml @17:48:11.77 CSV.dial CBV @17:48:11.81
CBV.dial CSV @17:48:13.11 CTV.dial :air @17:48:14.73 CS.seek 0ml @17:48:41.77 delay 7 seconds @17:48:48.81 CS.seek 3ml @17:48:57.55 CSV.dial :waste @17:48:58.65 CS.seek 0ml @17:49:07.33 Flushing upper manifold @17:49:07.49 CSV.dial :flush @17:49:08.04 CS.seek 5ml @17:49:22.02 delay 5 seconds @17:49:27.05 CSV.dial :air @17:49:27.67 CS.seek 7ml @17:49:33.89 CSV.dial CBV @17:49:33.93
CBV.dial CSV @17:49:35.21 CTV.dial :air @17:49:35.24 CS.seek 0ml @17:49:40.92 delay 1:00 @17:49:54.36
delay 7 seconds @17:50:01.40 CS.seek 3ml @17:50:10.08 CSV.dial :waste @17:50:10.65 CS.seek 0ml @17:50:19.45 CSV.dial :flush @17:50:20.01 CS.seek 3ml @17:50:28.71 delay 5 seconds @17:50:33.74 CSV.dial :air @17:50:34.37 CS.seek 5ml @17:50:40.52 CSV.dial :air @17:50:40.55 CS.seek 0ml @17:50:40.94 AFV.select :carrierSolution @17:50:41.06 delay 2 seconds @17:50:43.09 AS.reconfigure ASconfig @17:50:43.16 AS.seek 1ml @17:50:54.39
delay 7 seconds @17:51:01.43 CS.seek 3ml @17:51:10.11 CSV.dial :waste @17:51:11.07 CS.seek 0ml @17:51:19.89 Flushing lower manifold's waste1 port @17:51:20.05 CSV.dial :flush @17:51:20.62 CS.seek 5ml @17:51:34.68 delay 5 seconds @17:51:39.71 CSV.dial :air @17:51:40.36 CS.seek 7ml @17:51:40.95 delay 1 second @17:51:41.97 AFV.select :holdingCoil @17:51:42.08 delay 1 second @17:51:43.11 ATV.dial ASV @17:51:43.12 ASV.dial :wasteR @17:51:43.16 AS.reconfigure ASslow1 @17:51:43.22 AS.seek 0ml @17:51:46.46 CSV.dial CTV @17:51:46.50
CTV.dial CSV @17:51:48.19 CBV.dial :waste1 @17:51:48.78 CS.seek 0ml @17:52:07.80 Flushing lower manifold's waste2b port @17:52:07.96 CSV.dial :flush @17:52:08.89 CS.seek 5ml @17:52:22.80 delay 5 seconds @17:52:27.83 CSV.dial :air @17:52:28.47 CS.seek 7ml @17:52:34.71 CSV.dial CTV @17:52:34.75
CTV.dial CSV @17:52:35.30 CBV.dial :waste2b @17:52:36.25 CS.seek 0ml @17:52:55.30 Garaging FlushPuck in Collection Clamp @17:52:55.41 Elbow.reconfigure TightElbow @17:52:55.48 Elbow.seek CC @17:52:55.74 Elbow.reconfigure LooseElbow @17:52:55.88 Forearm.seek CC @17:52:56.95 Hand.close @17:52:57.72 CC.seek :open @17:53:02.12 Forearm.seek :retract @17:53:03.19 Elbow.reconfigure TightElbow @17:53:03.26 Elbow.seek :garage @17:53:07.04 Elbow.reconfigure LooseElbow @17:53:07.17 Forearm.reconfigure TightForearm @17:53:07.24 Forearm.seek :garage @17:53:08.44 Hand.open @17:53:09.29 Forearm.reconfigure LooseForearm @17:53:09.36 Forearm.seek :retract @17:53:10.42 Completed [mbaPhase] begun 16:22:44 @17:53:10.44 Extending 20invasivedeploymfbtest until completion of MFBPCR @17:55:04.63 delay 20 seconds @17:55:24.66 ATV.dial :air3 @17:55:26.23 AS.seek 0.59ml @17:57:25.20 delay 3 seconds @17:57:28.23 ATV.dial :ASV @17:57:29.69 ASV.dial :wasteR @17:57:29.86 AS.seek 0.09ml @17:59:10.75 delay 30 seconds @17:59:40.78 ATV.dial :extSrc @17:59:41.58 delay 3 seconds @17:59:44.61 ATV.dial :ElutionWater @17:59:45.77 AS.seek 0.12ml @17:59:52.11 ATV.dial :air3 @17:59:52.77 AS.seek 0.13ml @17:59:55.06 delay 2 seconds @17:59:57.08 ATV.dial :bleach @17:59:57.57 AS.seek 0.23ml @18:00:17.97 delay 3 seconds @18:00:21.00 ATV.dial :air3 @18:00:21.58 delay 3 seconds @18:00:24.68 AS.seek 0.255ml @18:00:29.98 delay 3 seconds @18:00:33.01 ATV.dial :bleach @18:00:33.66 AS.seek 0.24ml @18:00:37.00 delay 3 seconds @18:00:40.03 ATV.dial :ASV @18:00:41.09 ASV.dial :SPEdelivery @18:00:41.48 AFV.select :carrierSolution @18:00:41.60 delay 2 seconds @18:00:43.63 AS.reconfigure ASconfig @18:00:43.70 AS.seek 0.5ml @18:00:58.95 delay 1 second @18:00:59.97 AFV.select :holdingCoil @18:01:00.13 AS.reconfigure ASslow1 @18:01:00.20 AS.seek 0.17ml @18:02:06.87 SPE.hold 2:46:40,95C @18:02:06.92 delay 3:00 @18:05:06.96 AFV.select :carrierSolution @18:05:07.08 delay 2 seconds @18:05:09.11 AS.reconfigure ASconfig @18:05:09.18 AS.seek 1ml @18:05:57.19 delay 1 second @18:05:58.21 AFV.select :holdingCoil @18:05:58.32 delay 1 second @18:05:59.35 ASV.dial :wasteD @18:06:00.48 AS.seek 0ml @18:06:58.28 Priming Eluate @18:06:58.29 AFV.select :carrierSolution @18:06:58.43 delay 2 seconds @18:07:00.47 AS.seek 0.5ml @18:07:29.48 delay 1 second @18:07:30.50 AFV.select :holdingCoil @18:07:30.62 ATV.dial ASV @18:07:30.64 ASV.dial :SPErecovery @18:07:31.28 Swish #1 of 2 @18:07:31.37 AS.reconfigure ASslow0 @18:07:31.44 AS.seek 0.6ml @18:07:41.93 AS.seek 0.5ml @18:07:52.29 Swish #2 of 2 @18:07:52.39 AS.seek 0.6ml @18:08:02.96 AS.seek 0.5ml @18:08:13.37 delay 3 seconds @18:08:16.45 AS.reconfigure ASslow1 @18:08:16.53 AS.seek 0.684ml @18:08:53.84 delay 3 seconds @18:08:56.87 Eluate primed @18:08:56.88 ATV.dial :waste2 @18:08:57.56 AS.reconfigure ASnorm @18:08:57.63 AS.seek 0ml @18:09:32.46 SPE.coast @18:09:32.56 ASV.dial :SPErecovery @18:09:32.59 Setting up PCR for OM -- writing /var/log/esp/OM20may0418h99ml.pcr @18:09:32.63 Building PCR Slug from ASV with MM3 mix, PP5 primer @18:09:32.66 Priming with carrier @18:09:32.68 ATV.dial ARV @18:09:33.70 ARV.dial :carrier @18:09:34.07 AS.seek 0.5ml @18:09:59.61 AFV.select :carrierSolution @18:09:59.75 delay 2 seconds @18:10:01.78 AS.reconfigure ASconfig @18:10:01.85 AS.seek 1ml @18:10:30.89 delay 1 second @18:10:31.91 AFV.select :holdingCoil @18:10:32.03 delay 1 second @18:10:33.06 ATV.dial :waste @18:10:35.17 AS.reconfigure ASnorm @18:10:35.25 AS.seek :empty @18:11:26.05 AFV.select :carrierSolution @18:11:26.18 delay 2 seconds @18:11:28.21 AS.reconfigure ASconfig @18:11:28.28 AS.seek 0.5ml @18:11:57.39 delay 1 second @18:11:58.41 AFV.select :holdingCoil @18:11:58.53 Building Slug @18:11:58.54 ATV.dial :ARV @18:12:00.22 delay 2 seconds @18:12:02.25 ARV.dial :air1 @18:12:02.67 delay 2 seconds @18:12:04.76 AS.reconfigure ASslow2 @18:12:04.84 AS.seek 0.506ml @18:12:11.37 delay 2 seconds @18:12:13.40 ARV.dial :MM3 @18:12:14.47 delay 2 seconds @18:12:16.50 Adding MM3 @18:12:16.61 AS.seek 0.524ml @18:12:34.94 delay 25 seconds @18:12:59.97 ARV.dial :PP5 @18:13:00.38 Adding PP5 @18:13:00.40 delay 2 seconds @18:13:02.49 AS.seek 0.53ml @18:13:08.77 delay 10 seconds @18:13:18.80 ARV.dial :air3 @18:13:19.20 adding ARV air @18:13:19.22 delay 2 seconds @18:13:21.31 AS.seek 0.536ml @18:13:27.87 delay 5 seconds @18:13:32.90 ARV.dial :carrier @18:13:34.19 delay 7 seconds @18:13:41.28 AS.seek 0.594ml @18:14:40.02 delay 5 seconds @18:14:45.05 ATV.dial :ASV @18:14:45.47 delay 2 seconds @18:14:47.56 AS.seek 0.6ml @18:14:53.86 delay 4 seconds @18:14:57.89 ATV.dial ARV @18:14:58.47 delay 2 seconds @18:15:00.50 ARV.dial :carrier @18:15:00.51 delay 2 seconds @18:15:02.61 AS.seek 0.65ml @18:15:53.09 delay 4 seconds @18:15:57.12 ATV.dial PCR @18:15:57.75 ASV.dial :waste1 @18:15:58.56 ARV.dial :air1 @18:15:58.98 Positioning Slug by 0.324ml @18:15:59.09 AS.seek 0.326ml @18:21:25.44 delay 5 seconds @18:21:30.47 ATV.dial ARV @18:21:31.27 ASV.dial :PCRlock @18:21:31.67 delay 5 seconds @18:21:36.70 Running OM Profile60C350 thermocycling @18:21:36.72 PCR.program Profile60C350 @18:21:36.86 Profile60C350 should complete in < 2:02:45 @18:21:36.88 PCR.run 2:38:26.25 @19:59:17.08 PCR.off @19:59:18.37 PCR for OM completed -- closed /var/log/esp/OM20may0418h99ml.pcr @19:59:18.46 Cleaning PCR @19:59:18.47 AFV.select :carrierSolution @19:59:18.61 delay 2 seconds @19:59:20.64 AS.reconfigure ASconfig @19:59:20.71 AS.seek 0.5ml @19:59:31.00 delay 1 second @19:59:32.02 AFV.select :holdingCoil @19:59:32.14 ASV.dial :waste1 @19:59:32.55 ATV.dial :air3 @19:59:33.88 AS.reconfigure ASslow1 @19:59:33.95 AS.seek 0.51ml @19:59:36.29 delay 2 seconds @19:59:38.31 ATV.dial :bleach @19:59:38.96 AS.seek 0.81ml @20:00:39.59 delay 3 seconds @20:00:42.62 ATV.dial :air3 @20:00:43.20 delay 3 seconds @20:00:46.29 AS.seek 0.835ml @20:00:51.58 delay 3 seconds @20:00:54.61 ATV.dial :bleach @20:00:55.26 AS.seek 0.82ml @20:00:58.62 delay 3 seconds @20:01:01.65 ARV.dial :waste @20:01:02.28 delay 3 seconds @20:01:05.31 ATV.dial :ARV @20:01:06.23 AS.reconfigure ASconfig @20:01:06.30 AS.seek 0.47ml @20:01:26.74 AFV.select :carrierSolution @20:01:26.88 delay 2 seconds @20:01:28.92 AS.seek 1ml @20:01:59.69 delay 1 second @20:02:00.71 AFV.select :holdingCoil @20:02:00.84 delay 1 second @20:02:01.88 AS.reconfigure ASfast @20:02:01.95 AS.seek 0ml @20:02:29.31 AFV.select :carrierSolution @20:02:29.43 delay 2 seconds @20:02:31.46 AS.reconfigure ASconfig @20:02:31.53 AS.seek 1ml @20:03:29.36 delay 1 second @20:03:30.38 AFV.select :holdingCoil @20:03:30.50 delay 1 second @20:03:31.53 AS.reconfigure ASfast @20:03:31.60 AS.seek 0ml @20:03:58.97 AFV.select :carrierSolution @20:03:59.10 delay 2 seconds @20:04:01.13 AS.reconfigure ASconfig @20:04:01.20 AS.seek 1ml @20:04:58.97 delay 1 second @20:04:59.99 AFV.select :holdingCoil @20:05:00.11 delay 1 second @20:05:01.14 AS.reconfigure ASfast @20:05:01.21 AS.seek 0ml @20:05:28.58 ARV.dial between carrier and air1 @20:05:29.09 ATV.dial :air3 @20:05:30.43 AS.reconfigure ASslow1 @20:05:30.50 AS.seek 0.01ml @20:05:32.87 delay 2 seconds @20:05:34.89 ATV.dial :bleach @20:05:35.54 AS.seek 0.81ml @20:08:16.78 delay 3 seconds @20:08:19.80 ATV.dial :air3 @20:08:20.37 delay 3 seconds @20:08:23.46 AS.seek 0.835ml @20:08:28.78 delay 3 seconds @20:08:31.81 ATV.dial :bleach @20:08:33.04 AS.seek 0.82ml @20:08:36.37 delay 3 seconds @20:08:39.40 ATV.dial PCR @20:08:39.81 AS.reconfigure ASconfig @20:08:39.88 AS.seek :empty @20:09:27.37 PCR.program :bleachProfile @20:09:27.43 PCR program should complete in < 2:45 @20:09:27.45 PCR.run 10:00 @20:12:23.23 PCR.off @20:12:23.27 AFV.select :carrierSolution @20:12:23.39 delay 2 seconds @20:12:25.43 AS.seek 1ml @20:13:23.28 delay 1 second @20:13:24.30 AFV.select :holdingCoil @20:13:24.42 delay 1 second @20:13:25.47 AS.reconfigure ASfast @20:13:25.54 AS.seek 0ml @20:13:52.92 delay 3 seconds @20:13:55.95 AFV.select :carrierSolution @20:13:56.07 delay 2 seconds @20:13:58.10 AS.reconfigure ASconfig @20:13:58.18 AS.seek 1ml @20:14:55.98 delay 1 second @20:14:57.00 AFV.select :holdingCoil @20:14:57.12 delay 1 second @20:14:58.15 AS.reconfigure ASfast @20:14:58.22 AS.seek 0ml @20:15:25.58 delay 3 seconds @20:15:28.61 AFV.select :carrierSolution @20:15:28.73 delay 2 seconds @20:15:30.76 AS.reconfigure ASconfig @20:15:30.83 AS.seek 1ml @20:16:28.62 delay 1 second @20:16:29.64 AFV.select :holdingCoil @20:16:29.76 delay 1 second @20:16:30.79 AS.reconfigure ASfast @20:16:30.86 AS.seek 0ml @20:16:58.22 delay 3 seconds @20:17:01.25 AFV.select :carrierSolution @20:17:01.37 delay 2 seconds @20:17:03.40 AS.reconfigure ASconfig @20:17:03.48 AS.seek 1ml @20:18:01.30 delay 1 second @20:18:02.32 AFV.select :holdingCoil @20:18:02.44 delay 1 second @20:18:03.47 AS.reconfigure ASfast @20:18:03.54 AS.seek 0ml @20:18:30.91 delay 3 seconds @20:18:33.94 AFV.select :carrierSolution @20:18:34.06 delay 2 seconds @20:18:36.09 AS.reconfigure ASconfig @20:18:36.16 AS.seek 1ml @20:19:33.97 delay 1 second @20:19:34.99 AFV.select :holdingCoil @20:19:35.11 delay 1 second @20:19:36.14 AS.reconfigure ASnorm @20:19:36.21 AS.seek 0ml @20:20:26.96 Setting up PCR for CG -- writing /var/log/esp/CG20may0420h99ml.pcr @20:20:26.98 Building PCR Slug from ASV with MM1 mix, PP2 primer @20:20:27.00 Priming with carrier @20:20:27.03 ATV.dial ARV @20:20:27.69 ARV.dial :carrier @20:20:28.05 AS.seek 0.5ml @20:20:53.58 AFV.select :carrierSolution @20:20:53.71 delay 2 seconds @20:20:55.74 AS.reconfigure ASconfig @20:20:55.81 AS.seek 1ml @20:21:24.86 delay 1 second @20:21:25.88 AFV.select :holdingCoil @20:21:26.00 delay 1 second @20:21:27.03 ATV.dial :waste @20:21:28.72 AS.reconfigure ASnorm @20:21:28.80 AS.seek :empty @20:22:19.59 AFV.select :carrierSolution @20:22:19.72 delay 2 seconds @20:22:21.75 AS.reconfigure ASconfig @20:22:21.82 AS.seek 0.5ml @20:22:50.91 delay 1 second @20:22:51.94 AFV.select :holdingCoil @20:22:52.06 Building Slug @20:22:52.07 ATV.dial :ARV @20:22:53.75 delay 2 seconds @20:22:55.78 ARV.dial :air1 @20:22:56.20 delay 2 seconds @20:22:58.28 AS.reconfigure ASslow2 @20:22:58.36 AS.seek 0.506ml @20:23:04.70 delay 2 seconds @20:23:06.72 ARV.dial :MM1 @20:23:07.33 delay 2 seconds @20:23:09.36 Adding MM1 @20:23:09.45 AS.seek 0.524ml @20:23:27.78 delay 25 seconds @20:23:52.81 ARV.dial :PP2 @20:23:53.22 Adding PP2 @20:23:53.24 delay 2 seconds @20:23:55.33 AS.seek 0.53ml @20:24:01.56 delay 10 seconds @20:24:11.59 ARV.dial :air2 @20:24:12.00 adding ARV air @20:24:12.60 delay 2 seconds @20:24:14.72 AS.seek 0.536ml @20:24:20.97 delay 5 seconds @20:24:26.00 ARV.dial :carrier @20:24:27.30 delay 7 seconds @20:24:34.40 AS.seek 0.594ml @20:25:33.12 delay 5 seconds @20:25:38.15 ATV.dial :ASV @20:25:38.56 delay 2 seconds @20:25:40.65 AS.seek 0.6ml @20:25:46.92 delay 4 seconds @20:25:50.95 ATV.dial ARV @20:25:51.53 delay 2 seconds @20:25:53.56 ARV.dial :carrier @20:25:53.58 delay 2 seconds @20:25:55.68 AS.seek 0.65ml @20:26:46.19 delay 4 seconds @20:26:50.22 ATV.dial PCR @20:26:50.86 ASV.dial :waste1 @20:26:50.88 ARV.dial :air1 @20:26:51.32 Positioning Slug by 0.324ml @20:26:51.43 AS.seek 0.326ml @20:32:17.48 delay 5 seconds @20:32:22.51 ATV.dial ARV @20:32:23.31 ASV.dial :PCRlock @20:32:23.73 delay 5 seconds @20:32:28.76 Running CG Profile60C300 thermocycling @20:32:28.77 PCR.program Profile60C300 @20:32:28.90 Profile60C300 should complete in < 1:59:25 @20:32:28.92 PCR.run 2:34:16.25 @22:09:00.31 PCR.off @22:09:00.99 PCR for CG completed -- closed /var/log/esp/CG20may0420h99ml.pcr @22:09:01.08 Cleaning PCR @22:09:01.09 AFV.select :carrierSolution @22:09:01.23 delay 2 seconds @22:09:03.26 AS.reconfigure ASconfig @22:09:03.33 AS.seek 0.5ml @22:09:13.64 delay 1 second @22:09:14.66 AFV.select :holdingCoil @22:09:14.78 ASV.dial :waste1 @22:09:15.19 ATV.dial :air3 @22:09:16.50 AS.reconfigure ASslow1 @22:09:16.58 AS.seek 0.51ml @22:09:18.88 delay 2 seconds @22:09:20.90 ATV.dial :bleach @22:09:21.55 AS.seek 0.81ml @22:10:22.17 delay 3 seconds @22:10:25.20 ATV.dial :air3 @22:10:25.78 delay 3 seconds @22:10:28.87 AS.seek 0.835ml @22:10:34.28 delay 3 seconds @22:10:37.31 ATV.dial :bleach @22:10:37.96 AS.seek 0.82ml @22:10:41.22 delay 3 seconds @22:10:44.24 ARV.dial :waste @22:10:45.46 delay 3 seconds @22:10:48.49 ATV.dial :ARV @22:10:49.41 AS.reconfigure ASconfig @22:10:49.48 AS.seek 0.47ml @22:11:09.90 AFV.select :carrierSolution @22:11:10.04 delay 2 seconds @22:11:12.08 AS.seek 1ml @22:11:42.89 delay 1 second @22:11:43.91 AFV.select :holdingCoil @22:11:44.03 delay 1 second @22:11:45.06 AS.reconfigure ASfast @22:11:45.13 AS.seek 0ml @22:12:12.50 AFV.select :carrierSolution @22:12:12.63 delay 2 seconds @22:12:14.66 AS.reconfigure ASconfig @22:12:14.73 AS.seek 1ml @22:13:12.55 delay 1 second @22:13:13.57 AFV.select :holdingCoil @22:13:13.69 delay 1 second @22:13:14.72 AS.reconfigure ASfast @22:13:14.79 AS.seek 0ml @22:13:42.14 AFV.select :carrierSolution @22:13:42.26 delay 2 seconds @22:13:44.29 AS.reconfigure ASconfig @22:13:44.36 AS.seek 1ml @22:14:42.12 delay 1 second @22:14:43.14 AFV.select :holdingCoil @22:14:43.26 delay 1 second @22:14:44.29 AS.reconfigure ASfast @22:14:44.36 AS.seek 0ml @22:15:11.73 ARV.dial between carrier and air1 @22:15:12.25 ATV.dial :air3 @22:15:13.57 AS.reconfigure ASslow1 @22:15:13.64 AS.seek 0.01ml @22:15:15.98 delay 2 seconds @22:15:18.00 ATV.dial :bleach @22:15:18.65 AS.seek 0.81ml @22:17:59.81 delay 3 seconds @22:18:02.84 ATV.dial :air3 @22:18:03.42 delay 3 seconds @22:18:06.51 AS.seek 0.835ml @22:18:11.80 delay 3 seconds @22:18:14.83 ATV.dial :bleach @22:18:15.48 AS.seek 0.82ml @22:18:18.81 delay 3 seconds @22:18:21.84 ATV.dial PCR @22:18:22.25 AS.reconfigure ASconfig @22:18:22.31 AS.seek :empty @22:19:09.81 PCR.program :bleachProfile @22:19:09.87 PCR program should complete in < 2:45 @22:19:09.89 PCR.run 10:00 @22:22:05.67 PCR.off @22:22:05.71 AFV.select :carrierSolution @22:22:05.83 delay 2 seconds @22:22:07.87 AS.seek 1ml @22:23:05.69 delay 1 second @22:23:06.71 AFV.select :holdingCoil @22:23:06.83 delay 1 second @22:23:07.86 AS.reconfigure ASfast @22:23:07.93 AS.seek 0ml @22:23:35.28 delay 3 seconds @22:23:38.31 AFV.select :carrierSolution @22:23:38.43 delay 2 seconds @22:23:40.46 AS.reconfigure ASconfig @22:23:40.54 AS.seek 1ml @22:24:38.30 delay 1 second @22:24:39.32 AFV.select :holdingCoil @22:24:39.44 delay 1 second @22:24:40.47 AS.reconfigure ASfast @22:24:40.54 AS.seek 0ml @22:25:07.91 delay 3 seconds @22:25:10.94 AFV.select :carrierSolution @22:25:11.06 delay 2 seconds @22:25:13.09 AS.reconfigure ASconfig @22:25:13.17 AS.seek 1ml @22:26:10.97 delay 1 second @22:26:11.99 AFV.select :holdingCoil @22:26:12.11 delay 1 second @22:26:13.14 AS.reconfigure ASfast @22:26:13.21 AS.seek 0ml @22:26:40.58 delay 3 seconds @22:26:43.61 AFV.select :carrierSolution @22:26:43.73 delay 2 seconds @22:26:45.76 AS.reconfigure ASconfig @22:26:45.83 AS.seek 1ml @22:27:43.61 delay 1 second @22:27:44.63 AFV.select :holdingCoil @22:27:44.75 delay 1 second @22:27:45.78 AS.reconfigure ASfast @22:27:45.85 AS.seek 0ml @22:28:13.23 delay 3 seconds @22:28:16.26 AFV.select :carrierSolution @22:28:16.38 delay 2 seconds @22:28:18.41 AS.reconfigure ASconfig @22:28:18.49 AS.seek 1ml @22:29:16.25 delay 1 second @22:29:17.27 AFV.select :holdingCoil @22:29:17.38 delay 1 second @22:29:18.41 AS.reconfigure ASnorm @22:29:18.48 AS.seek 0ml @22:30:09.18 Setting up PCR for ML -- writing /var/log/esp/ML20may0422h99ml.pcr @22:30:09.20 Building PCR Slug from ASV with MM2 mix, PP4 primer @22:30:09.23 Priming with carrier @22:30:09.25 ATV.dial ARV @22:30:10.50 ARV.dial :carrier @22:30:10.87 AS.seek 0.5ml @22:30:36.51 AFV.select :carrierSolution @22:30:36.65 delay 2 seconds @22:30:38.68 AS.reconfigure ASconfig @22:30:38.76 AS.seek 1ml @22:31:07.77 delay 1 second @22:31:08.79 AFV.select :holdingCoil @22:31:08.91 delay 1 second @22:31:09.94 ATV.dial :waste @22:31:11.64 AS.reconfigure ASnorm @22:31:11.72 AS.seek :empty @22:32:02.48 AFV.select :carrierSolution @22:32:02.61 delay 2 seconds @22:32:04.64 AS.reconfigure ASconfig @22:32:04.71 AS.seek 0.5ml @22:32:33.81 delay 1 second @22:32:34.83 AFV.select :holdingCoil @22:32:34.95 Building Slug @22:32:34.96 ATV.dial :ARV @22:32:36.64 delay 2 seconds @22:32:38.67 ARV.dial :air1 @22:32:39.07 delay 2 seconds @22:32:41.15 AS.reconfigure ASslow2 @22:32:41.23 AS.seek 0.506ml @22:32:47.66 delay 2 seconds @22:32:49.68 ARV.dial :MM2 @22:32:51.19 delay 2 seconds @22:32:53.22 Adding MM2 @22:32:53.32 AS.seek 0.524ml @22:33:11.66 delay 25 seconds @22:33:36.69 ARV.dial :PP4 @22:33:37.11 Adding PP4 @22:33:37.13 delay 2 seconds @22:33:39.22 AS.seek 0.53ml @22:33:45.50 delay 10 seconds @22:33:55.53 ARV.dial :air3 @22:33:55.94 adding ARV air @22:33:55.96 delay 2 seconds @22:33:58.05 AS.seek 0.536ml @22:34:04.69 delay 5 seconds @22:34:09.72 ARV.dial :carrier @22:34:11.00 delay 7 seconds @22:34:18.09 AS.seek 0.594ml @22:35:16.69 delay 5 seconds @22:35:21.72 ATV.dial :ASV @22:35:22.12 delay 2 seconds @22:35:24.21 AS.seek 0.6ml @22:35:30.47 delay 4 seconds @22:35:34.50 ATV.dial ARV @22:35:35.06 delay 2 seconds @22:35:37.09 ARV.dial :carrier @22:35:37.10 delay 2 seconds @22:35:39.20 AS.seek 0.65ml @22:36:29.83 delay 4 seconds @22:36:33.86 ATV.dial PCR @22:36:34.48 ASV.dial :waste1 @22:36:34.50 ARV.dial :air1 @22:36:35.04 Positioning Slug by 0.324ml @22:36:35.15 AS.seek 0.326ml @22:42:01.39 delay 5 seconds @22:42:06.42 ATV.dial ARV @22:42:07.22 ASV.dial :PCRlock @22:42:07.64 delay 5 seconds @22:42:12.67 Running ML Profile60C350 thermocycling @22:42:12.69 PCR.program Profile60C350 @22:42:12.82 Profile60C350 should complete in < 2:02:45 @22:42:12.84 PCR.run 2:38:26.25 @00:19:29.16CEST05-May-20 PCR.off @00:19:30.54 PCR for ML completed -- closed /var/log/esp/ML20may0422h99ml.pcr @00:19:30.63 Cleaning PCR @00:19:30.64 AFV.select :carrierSolution @00:19:30.78 delay 2 seconds @00:19:32.81 AS.reconfigure ASconfig @00:19:32.88 AS.seek 0.5ml @00:19:43.19 delay 1 second @00:19:44.21 AFV.select :holdingCoil @00:19:44.33 ASV.dial :waste1 @00:19:44.75 ATV.dial :air3 @00:19:46.09 AS.reconfigure ASslow1 @00:19:46.16 AS.seek 0.51ml @00:19:48.47 delay 2 seconds @00:19:50.50 ATV.dial :bleach @00:19:51.15 AS.seek 0.81ml @00:20:51.83 delay 3 seconds @00:20:54.86 ATV.dial :air3 @00:20:55.44 delay 3 seconds @00:20:58.53 AS.seek 0.835ml @00:21:03.84 delay 3 seconds @00:21:06.87 ATV.dial :bleach @00:21:07.52 AS.seek 0.82ml @00:21:10.80 delay 3 seconds @00:21:13.83 ARV.dial :waste @00:21:14.44 delay 3 seconds @00:21:17.47 ATV.dial :ARV @00:21:18.37 AS.reconfigure ASconfig @00:21:18.45 AS.seek 0.47ml @00:21:38.82 AFV.select :carrierSolution @00:21:38.96 delay 2 seconds @00:21:41.00 AS.seek 1ml @00:22:11.75 delay 1 second @00:22:12.77 AFV.select :holdingCoil @00:22:12.89 delay 1 second @00:22:13.92 AS.reconfigure ASfast @00:22:13.99 AS.seek 0ml @00:22:41.35 AFV.select :carrierSolution @00:22:41.49 delay 2 seconds @00:22:43.52 AS.reconfigure ASconfig @00:22:43.59 AS.seek 1ml @00:23:41.39 delay 1 second @00:23:42.41 AFV.select :holdingCoil @00:23:42.53 delay 1 second @00:23:43.56 AS.reconfigure ASfast @00:23:43.63 AS.seek 0ml @00:24:11.00 AFV.select :carrierSolution @00:24:11.13 delay 2 seconds @00:24:13.16 AS.reconfigure ASconfig @00:24:13.23 AS.seek 1ml @00:25:10.98 delay 1 second @00:25:12.00 AFV.select :holdingCoil @00:25:12.12 delay 1 second @00:25:13.15 AS.reconfigure ASfast @00:25:13.22 AS.seek 0ml @00:25:40.59 ARV.dial between carrier and air1 @00:25:41.09 ATV.dial :air3 @00:25:42.43 AS.reconfigure ASslow1 @00:25:42.50 AS.seek 0.01ml @00:25:44.84 delay 2 seconds @00:25:46.86 ATV.dial :bleach @00:25:47.51 AS.seek 0.81ml @00:28:28.77 delay 3 seconds @00:28:31.80 ATV.dial :air3 @00:28:32.39 delay 3 seconds @00:28:35.48 AS.seek 0.835ml @00:28:40.92 delay 3 seconds @00:28:43.94 ATV.dial :bleach @00:28:44.59 AS.seek 0.82ml @00:28:47.89 delay 3 seconds @00:28:50.91 ATV.dial PCR @00:28:51.33 AS.reconfigure ASconfig @00:28:51.39 AS.seek :empty @00:29:38.88 PCR.program :bleachProfile @00:29:39.50 PCR program should complete in < 2:45 @00:29:39.53 PCR.run 10:00 @00:32:35.31 PCR.off @00:32:35.35 AFV.select :carrierSolution @00:32:35.47 delay 2 seconds @00:32:37.51 AS.seek 1ml @00:33:35.34 delay 1 second @00:33:36.36 AFV.select :holdingCoil @00:33:36.48 delay 1 second @00:33:37.51 AS.reconfigure ASfast @00:33:37.58 AS.seek 0ml @00:34:04.94 delay 3 seconds @00:34:07.97 AFV.select :carrierSolution @00:34:08.09 delay 2 seconds @00:34:10.12 AS.reconfigure ASconfig @00:34:10.20 AS.seek 1ml @00:35:08.00 delay 1 second @00:35:09.02 AFV.select :holdingCoil @00:35:09.14 delay 1 second @00:35:10.17 AS.reconfigure ASfast @00:35:10.24 AS.seek 0ml @00:35:37.59 delay 3 seconds @00:35:40.62 AFV.select :carrierSolution @00:35:40.74 delay 2 seconds @00:35:42.77 AS.reconfigure ASconfig @00:35:42.85 AS.seek 1ml @00:36:40.66 delay 1 second @00:36:41.68 AFV.select :holdingCoil @00:36:41.80 delay 1 second @00:36:42.83 AS.reconfigure ASfast @00:36:42.90 AS.seek 0ml @00:37:10.25 delay 3 seconds @00:37:13.28 AFV.select :carrierSolution @00:37:13.40 delay 2 seconds @00:37:15.43 AS.reconfigure ASconfig @00:37:15.50 AS.seek 1ml @00:38:13.30 delay 1 second @00:38:14.32 AFV.select :holdingCoil @00:38:14.44 delay 1 second @00:38:15.47 AS.reconfigure ASfast @00:38:15.54 AS.seek 0ml @00:38:42.91 delay 3 seconds @00:38:45.94 AFV.select :carrierSolution @00:38:46.06 delay 2 seconds @00:38:48.09 AS.reconfigure ASconfig @00:38:48.17 AS.seek 1ml @00:39:45.97 delay 1 second @00:39:46.99 AFV.select :holdingCoil @00:39:47.11 delay 1 second @00:39:48.14 AS.reconfigure ASnorm @00:39:48.21 AS.seek 0ml @00:40:38.93 Setting up PCR for NM -- writing /var/log/esp/NM20may0500h99ml.pcr @00:40:38.95 Building PCR Slug from ASV with MM1 mix, PP1 primer @00:40:38.98 Priming with carrier @00:40:39.00 ATV.dial ARV @00:40:39.64 ARV.dial :carrier @00:40:40.01 AS.seek 0.5ml @00:41:05.60 AFV.select :carrierSolution @00:41:05.73 delay 2 seconds @00:41:07.76 AS.reconfigure ASconfig @00:41:07.83 AS.seek 1ml @00:41:36.86 delay 1 second @00:41:37.88 AFV.select :holdingCoil @00:41:38.00 delay 1 second @00:41:39.03 ATV.dial :waste @00:41:40.72 AS.reconfigure ASnorm @00:41:40.79 AS.seek :empty @00:42:31.54 AFV.select :carrierSolution @00:42:31.68 delay 2 seconds @00:42:33.71 AS.reconfigure ASconfig @00:42:33.78 AS.seek 0.5ml @00:43:02.88 delay 1 second @00:43:03.90 AFV.select :holdingCoil @00:43:04.02 Building Slug @00:43:04.04 ATV.dial :ARV @00:43:05.70 delay 2 seconds @00:43:07.73 ARV.dial :air1 @00:43:08.14 delay 2 seconds @00:43:10.22 AS.reconfigure ASslow2 @00:43:10.30 AS.seek 0.506ml @00:43:16.92 delay 2 seconds @00:43:18.94 ARV.dial :MM1 @00:43:19.55 delay 2 seconds @00:43:21.58 Adding MM1 @00:43:21.67 AS.seek 0.524ml @00:43:39.94 delay 25 seconds @00:44:04.97 ARV.dial :PP1 @00:44:05.36 Adding PP1 @00:44:05.37 delay 2 seconds @00:44:07.47 AS.seek 0.53ml @00:44:13.67 delay 10 seconds @00:44:23.70 ARV.dial :air1 @00:44:24.11 adding ARV air @00:44:24.13 delay 2 seconds @00:44:26.22 AS.seek 0.536ml @00:44:32.45 delay 5 seconds @00:44:37.48 ARV.dial :carrier @00:44:37.91 delay 7 seconds @00:44:45.00 AS.seek 0.594ml @00:45:44.08 delay 5 seconds @00:45:49.11 ATV.dial :ASV @00:45:49.53 delay 2 seconds @00:45:51.62 AS.seek 0.6ml @00:45:57.92 delay 4 seconds @00:46:01.95 ATV.dial ARV @00:46:02.53 delay 2 seconds @00:46:04.56 ARV.dial :carrier @00:46:04.58 delay 2 seconds @00:46:06.67 AS.seek 0.65ml @00:46:57.42 delay 4 seconds @00:47:01.45 ATV.dial PCR @00:47:02.08 ASV.dial :waste1 @00:47:02.10 ARV.dial :air1 @00:47:02.54 Positioning Slug by 0.324ml @00:47:02.65 AS.seek 0.326ml @00:52:28.91 delay 5 seconds @00:52:33.94 ATV.dial ARV @00:52:34.74 ASV.dial :PCRlock @00:52:35.16 delay 5 seconds @00:52:40.19 Running NM Profile60C300 thermocycling @00:52:40.20 PCR.program Profile60C300 @00:52:40.33 Profile60C300 should complete in < 1:59:25 @00:52:40.35 PCR.run 2:34:16.25 @02:28:55.84 PCR.off @02:28:56.52 PCR for NM completed -- closed /var/log/esp/NM20may0500h99ml.pcr @02:28:56.61 Cleaning PCR @02:28:56.62 AFV.select :carrierSolution @02:28:56.76 delay 2 seconds @02:28:58.79 AS.reconfigure ASconfig @02:28:58.86 AS.seek 0.5ml @02:29:09.16 delay 1 second @02:29:10.18 AFV.select :holdingCoil @02:29:10.29 ASV.dial :waste1 @02:29:10.71 ATV.dial :air3 @02:29:12.03 AS.reconfigure ASslow1 @02:29:12.11 AS.seek 0.51ml @02:29:14.43 delay 2 seconds @02:29:16.45 ATV.dial :bleach @02:29:17.10 AS.seek 0.81ml @02:30:17.71 delay 3 seconds @02:30:20.74 ATV.dial :air3 @02:30:21.33 delay 3 seconds @02:30:24.42 AS.seek 0.835ml @02:30:29.71 delay 3 seconds @02:30:32.73 ATV.dial :bleach @02:30:33.38 AS.seek 0.82ml @02:30:36.74 delay 3 seconds @02:30:39.77 ARV.dial :waste @02:30:40.39 delay 3 seconds @02:30:43.42 ATV.dial :ARV @02:30:44.33 AS.reconfigure ASconfig @02:30:44.40 AS.seek 0.47ml @02:31:04.83 AFV.select :carrierSolution @02:31:04.96 delay 2 seconds @02:31:06.99 AS.seek 1ml @02:31:37.80 delay 1 second @02:31:38.82 AFV.select :holdingCoil @02:31:38.94 delay 1 second @02:31:39.97 AS.reconfigure ASfast @02:31:40.04 AS.seek 0ml @02:32:07.38 AFV.select :carrierSolution @02:32:08.09 delay 2 seconds @02:32:10.15 AS.reconfigure ASconfig @02:32:10.22 AS.seek 1ml @02:33:07.96 delay 1 second @02:33:08.98 AFV.select :holdingCoil @02:33:09.10 delay 1 second @02:33:10.13 AS.reconfigure ASfast @02:33:10.20 AS.seek 0ml @02:33:37.58 AFV.select :carrierSolution @02:33:37.71 delay 2 seconds @02:33:39.74 AS.reconfigure ASconfig @02:33:39.81 AS.seek 1ml @02:34:37.63 delay 1 second @02:34:38.65 AFV.select :holdingCoil @02:34:38.77 delay 1 second @02:34:39.80 AS.reconfigure ASfast @02:34:39.87 AS.seek 0ml @02:35:07.25 ARV.dial between carrier and air1 @02:35:07.77 ATV.dial :air3 @02:35:09.10 AS.reconfigure ASslow1 @02:35:09.17 AS.seek 0.01ml @02:35:11.50 delay 2 seconds @02:35:13.52 ATV.dial :bleach @02:35:14.17 AS.seek 0.81ml @02:37:55.35 delay 3 seconds @02:37:58.38 ATV.dial :air3 @02:37:58.96 delay 3 seconds @02:38:02.05 AS.seek 0.835ml @02:38:07.36 delay 3 seconds @02:38:10.39 ATV.dial :bleach @02:38:11.04 AS.seek 0.82ml @02:38:14.38 delay 3 seconds @02:38:17.41 ATV.dial PCR @02:38:17.81 AS.reconfigure ASconfig @02:38:17.88 AS.seek :empty @02:39:05.36 PCR.program :bleachProfile @02:39:05.43 PCR program should complete in < 2:45 @02:39:05.44 PCR.run 10:00 @02:42:01.23 PCR.off @02:42:01.27 AFV.select :carrierSolution @02:42:01.40 delay 2 seconds @02:42:03.44 AS.seek 1ml @02:43:01.28 delay 1 second @02:43:02.30 AFV.select :holdingCoil @02:43:02.42 delay 1 second @02:43:03.45 AS.reconfigure ASfast @02:43:03.52 AS.seek 0ml @02:43:30.88 delay 3 seconds @02:43:33.91 AFV.select :carrierSolution @02:43:34.03 delay 2 seconds @02:43:36.06 AS.reconfigure ASconfig @02:43:36.14 AS.seek 1ml @02:44:33.91 delay 1 second @02:44:34.93 AFV.select :holdingCoil @02:44:35.05 delay 1 second @02:44:36.08 AS.reconfigure ASfast @02:44:36.15 AS.seek 0ml @02:45:03.50 delay 3 seconds @02:45:06.53 AFV.select :carrierSolution @02:45:06.65 delay 2 seconds @02:45:08.68 AS.reconfigure ASconfig @02:45:08.75 AS.seek 1ml @02:46:06.56 delay 1 second @02:46:07.58 AFV.select :holdingCoil @02:46:07.70 delay 1 second @02:46:08.73 AS.reconfigure ASfast @02:46:08.80 AS.seek 0ml @02:46:36.16 delay 3 seconds @02:46:39.19 AFV.select :carrierSolution @02:46:39.31 delay 2 seconds @02:46:41.34 AS.reconfigure ASconfig @02:46:41.42 AS.seek 1ml @02:47:39.21 delay 1 second @02:47:40.23 AFV.select :holdingCoil @02:47:40.35 delay 1 second @02:47:41.38 AS.reconfigure ASfast @02:47:41.45 AS.seek 0ml @02:48:08.81 delay 3 seconds @02:48:11.84 AFV.select :carrierSolution @02:48:11.96 delay 2 seconds @02:48:13.99 AS.reconfigure ASconfig @02:48:14.07 AS.seek 1ml @02:49:11.88 delay 1 second @02:49:12.90 AFV.select :holdingCoil @02:49:13.02 delay 1 second @02:49:14.05 AS.reconfigure ASnorm @02:49:14.12 AS.seek 0ml @02:50:04.87 Setting up PCR for IPc -- writing /var/log/esp/IPc20may0502h99ml.pcr @02:50:04.89 Building PCR Slug from ASV with MM3 mix, PP6 primer @02:50:04.93 Priming with carrier @02:50:04.94 ATV.dial ARV @02:50:05.60 ARV.dial :carrier @02:50:05.96 AS.seek 0.5ml @02:50:31.52 AFV.select :carrierSolution @02:50:31.65 delay 2 seconds @02:50:33.68 AS.reconfigure ASconfig @02:50:33.75 AS.seek 1ml @02:51:02.78 delay 1 second @02:51:03.80 AFV.select :holdingCoil @02:51:03.92 delay 1 second @02:51:04.95 ATV.dial :waste @02:51:07.25 AS.reconfigure ASnorm @02:51:07.33 AS.seek :empty @02:51:58.14 AFV.select :carrierSolution @02:51:58.27 delay 2 seconds @02:52:00.30 AS.reconfigure ASconfig @02:52:00.37 AS.seek 0.5ml @02:52:29.49 delay 1 second @02:52:30.51 AFV.select :holdingCoil @02:52:30.63 Building Slug @02:52:30.65 ATV.dial :ARV @02:52:32.32 delay 2 seconds @02:52:34.35 ARV.dial :air1 @02:52:34.75 delay 2 seconds @02:52:36.83 AS.reconfigure ASslow2 @02:52:36.91 AS.seek 0.506ml @02:52:43.30 delay 2 seconds @02:52:45.33 ARV.dial :MM3 @02:52:46.39 delay 2 seconds @02:52:48.42 Adding MM3 @02:52:48.52 AS.seek 0.524ml @02:53:06.96 delay 25 seconds @02:53:31.99 ARV.dial :PP6 @02:53:32.41 Adding PP6 @02:53:32.43 delay 2 seconds @02:53:34.52 AS.seek 0.53ml @02:53:40.78 delay 10 seconds @02:53:50.81 ARV.dial :air1 @02:53:51.64 adding ARV air @02:53:51.66 delay 2 seconds @02:53:53.76 AS.seek 0.536ml @02:54:00.05 delay 5 seconds @02:54:05.08 ARV.dial :carrier @02:54:05.50 delay 7 seconds @02:54:12.59 AS.seek 0.594ml @02:55:11.08 delay 5 seconds @02:55:16.11 ATV.dial :ASV @02:55:16.53 delay 2 seconds @02:55:18.62 AS.seek 0.6ml @02:55:24.89 delay 4 seconds @02:55:28.92 ATV.dial ARV @02:55:29.60 delay 2 seconds @02:55:31.63 ARV.dial :carrier @02:55:31.65 delay 2 seconds @02:55:33.74 AS.seek 0.65ml @02:56:24.24 delay 4 seconds @02:56:28.27 ATV.dial PCR @02:56:28.91 ASV.dial :waste1 @02:56:28.93 ARV.dial :air1 @02:56:29.37 Positioning Slug by 0.324ml @02:56:29.48 AS.seek 0.326ml @03:01:55.82 delay 5 seconds @03:02:00.85 ATV.dial ARV @03:02:01.64 ASV.dial :PCRlock @03:02:02.07 delay 5 seconds @03:02:07.10 Running IPc ProfileIPc thermocycling @03:02:07.12 PCR.program ProfileIPc @03:02:07.24 ProfileIPc should complete in < 1:36:15 @03:02:07.26 PCR.run 2:05:18.75 @04:20:59.71 PCR.off @04:21:00.29 PCR for IPc completed -- closed /var/log/esp/IPc20may0502h99ml.pcr @04:21:00.38 Cleaning PCR @04:21:00.39 AFV.select :carrierSolution @04:21:00.52 delay 2 seconds @04:21:02.55 AS.reconfigure ASconfig @04:21:02.62 AS.seek 0.5ml @04:21:12.96 delay 1 second @04:21:13.98 AFV.select :holdingCoil @04:21:14.10 ASV.dial :waste1 @04:21:14.52 ATV.dial :air3 @04:21:15.86 AS.reconfigure ASslow1 @04:21:15.93 AS.seek 0.51ml @04:21:18.25 delay 2 seconds @04:21:20.27 ATV.dial :bleach @04:21:20.92 AS.seek 0.81ml @04:22:21.50 delay 3 seconds @04:22:24.53 ATV.dial :air3 @04:22:25.11 delay 3 seconds @04:22:28.20 AS.seek 0.835ml @04:22:33.50 delay 3 seconds @04:22:36.53 ATV.dial :bleach @04:22:37.18 AS.seek 0.82ml @04:22:40.46 delay 3 seconds @04:22:43.49 ARV.dial :waste @04:22:44.11 delay 3 seconds @04:22:47.14 ATV.dial :ARV @04:22:48.06 AS.reconfigure ASconfig @04:22:48.13 AS.seek 0.47ml @04:23:08.56 AFV.select :carrierSolution @04:23:08.70 delay 2 seconds @04:23:10.74 AS.seek 1ml @04:23:41.54 delay 1 second @04:23:42.56 AFV.select :holdingCoil @04:23:42.68 delay 1 second @04:23:43.71 AS.reconfigure ASfast @04:23:43.78 AS.seek 0ml @04:24:11.13 AFV.select :carrierSolution @04:24:11.26 delay 2 seconds @04:24:13.29 AS.reconfigure ASconfig @04:24:13.36 AS.seek 1ml @04:25:11.13 delay 1 second @04:25:12.15 AFV.select :holdingCoil @04:25:12.27 delay 1 second @04:25:13.30 AS.reconfigure ASfast @04:25:13.37 AS.seek 0ml @04:25:40.71 AFV.select :carrierSolution @04:25:40.85 delay 2 seconds @04:25:42.88 AS.reconfigure ASconfig @04:25:42.95 AS.seek 1ml @04:26:40.75 delay 1 second @04:26:41.77 AFV.select :holdingCoil @04:26:41.89 delay 1 second @04:26:42.92 AS.reconfigure ASfast @04:26:42.99 AS.seek 0ml @04:27:10.33 ARV.dial between carrier and air1 @04:27:10.85 ATV.dial :air3 @04:27:12.17 AS.reconfigure ASslow1 @04:27:12.24 AS.seek 0.01ml @04:27:14.58 delay 2 seconds @04:27:16.60 ATV.dial :bleach @04:27:17.25 AS.seek 0.81ml @04:29:58.41 delay 3 seconds @04:30:01.44 ATV.dial :air3 @04:30:02.02 delay 3 seconds @04:30:05.11 AS.seek 0.835ml @04:30:10.50 delay 3 seconds @04:30:13.53 ATV.dial :bleach @04:30:14.18 AS.seek 0.82ml @04:30:17.50 delay 3 seconds @04:30:20.53 ATV.dial PCR @04:30:20.94 AS.reconfigure ASconfig @04:30:21.01 AS.seek :empty @04:31:08.49 PCR.program :bleachProfile @04:31:08.55 PCR program should complete in < 2:45 @04:31:08.57 PCR.run 10:00 @04:34:04.95 PCR.off @04:34:04.99 AFV.select :carrierSolution @04:34:05.11 delay 2 seconds @04:34:07.15 AS.seek 1ml @04:35:04.96 delay 1 second @04:35:05.98 AFV.select :holdingCoil @04:35:06.10 delay 1 second @04:35:07.13 AS.reconfigure ASfast @04:35:07.20 AS.seek 0ml @04:35:34.58 delay 3 seconds @04:35:37.61 AFV.select :carrierSolution @04:35:37.73 delay 2 seconds @04:35:39.76 AS.reconfigure ASconfig @04:35:39.84 AS.seek 1ml @04:36:37.64 delay 1 second @04:36:38.66 AFV.select :holdingCoil @04:36:38.78 delay 1 second @04:36:39.81 AS.reconfigure ASfast @04:36:39.88 AS.seek 0ml @04:37:07.25 delay 3 seconds @04:37:10.28 AFV.select :carrierSolution @04:37:10.40 delay 2 seconds @04:37:12.43 AS.reconfigure ASconfig @04:37:12.51 AS.seek 1ml @04:38:10.27 delay 1 second @04:38:11.29 AFV.select :holdingCoil @04:38:11.41 delay 1 second @04:38:12.44 AS.reconfigure ASfast @04:38:12.51 AS.seek 0ml @04:38:39.88 delay 3 seconds @04:38:42.91 AFV.select :carrierSolution @04:38:43.03 delay 2 seconds @04:38:45.06 AS.reconfigure ASconfig @04:38:45.14 AS.seek 1ml @04:39:42.89 delay 1 second @04:39:43.91 AFV.select :holdingCoil @04:39:44.03 delay 1 second @04:39:45.06 AS.reconfigure ASfast @04:39:45.13 AS.seek 0ml @04:40:12.47 delay 3 seconds @04:40:15.50 AFV.select :carrierSolution @04:40:15.62 delay 2 seconds @04:40:17.65 AS.reconfigure ASconfig @04:40:17.72 AS.seek 1ml @04:41:15.52 delay 1 second @04:41:16.54 AFV.select :holdingCoil @04:41:16.66 delay 1 second @04:41:17.69 AS.reconfigure ASnorm @04:41:17.76 AS.seek 0ml @04:42:08.51 Setting up PCR for IPc2 -- writing /var/log/esp/IPc220may0504h99ml.pcr @04:42:08.53 Building PCR Slug from ASV with MM2 mix, PP3 primer @04:42:08.55 Priming with carrier @04:42:08.58 ATV.dial ARV @04:42:09.22 ARV.dial :carrier @04:42:09.57 AS.seek 0.5ml @04:42:35.15 AFV.select :carrierSolution @04:42:35.29 delay 2 seconds @04:42:37.32 AS.reconfigure ASconfig @04:42:37.39 AS.seek 1ml @04:43:06.49 delay 1 second @04:43:07.51 AFV.select :holdingCoil @04:43:07.63 delay 1 second @04:43:08.66 ATV.dial :waste @04:43:10.35 AS.reconfigure ASnorm @04:43:10.43 AS.seek :empty @04:44:01.20 AFV.select :carrierSolution @04:44:01.34 delay 2 seconds @04:44:03.37 AS.reconfigure ASconfig @04:44:03.44 AS.seek 0.5ml @04:44:32.54 delay 1 second @04:44:33.56 AFV.select :holdingCoil @04:44:33.68 Building Slug @04:44:33.69 ATV.dial :ARV @04:44:35.38 delay 2 seconds @04:44:37.41 ARV.dial :air1 @04:44:37.81 delay 2 seconds @04:44:39.90 AS.reconfigure ASslow2 @04:44:39.97 AS.seek 0.506ml @04:44:46.50 delay 2 seconds @04:44:48.52 ARV.dial :MM2 @04:44:50.04 delay 2 seconds @04:44:52.07 Adding MM2 @04:44:52.16 AS.seek 0.524ml @04:45:10.43 delay 25 seconds @04:45:35.46 ARV.dial :PP3 @04:45:35.88 Adding PP3 @04:45:35.90 delay 2 seconds @04:45:38.00 AS.seek 0.53ml @04:45:44.36 delay 10 seconds @04:45:54.39 ARV.dial :air2 @04:45:54.79 adding ARV air @04:45:54.81 delay 2 seconds @04:45:56.91 AS.seek 0.536ml @04:46:03.19 delay 5 seconds @04:46:08.22 ARV.dial :carrier @04:46:09.52 delay 7 seconds @04:46:16.61 AS.seek 0.594ml @04:47:15.15 delay 5 seconds @04:47:20.18 ATV.dial :ASV @04:47:20.60 delay 2 seconds @04:47:23.28 AS.seek 0.6ml @04:47:29.60 delay 4 seconds @04:47:33.63 ATV.dial ARV @04:47:34.32 delay 2 seconds @04:47:36.35 ARV.dial :carrier @04:47:36.37 delay 2 seconds @04:47:38.47 AS.seek 0.65ml @04:48:29.15 delay 4 seconds @04:48:33.18 ATV.dial PCR @04:48:33.82 ASV.dial :waste1 @04:48:33.84 ARV.dial :air1 @04:48:34.28 Positioning Slug by 0.324ml @04:48:34.39 AS.seek 0.326ml @04:54:00.44 delay 5 seconds @04:54:05.47 ATV.dial ARV @04:54:06.27 ASV.dial :PCRlock @04:54:06.69 delay 5 seconds @04:54:11.72 Running IPc2 ProfileIPc thermocycling @04:54:11.74 PCR.program ProfileIPc @04:54:11.86 ProfileIPc should complete in < 1:36:15 @04:54:11.88 PCR.run 2:05:18.75 @06:12:59.05 PCR.off @06:12:59.62 PCR for IPc2 completed -- closed /var/log/esp/IPc220may0504h99ml.pcr @06:12:59.64 Full MFB Decontamination @06:12:59.66 Decon extraction column and decon AIV @06:12:59.70 SPE.hold 2:46:40,55C @06:12:59.77 ASV.dial :SPEdelivery @06:13:00.44 delay 20 seconds @06:13:20.47 AFV.select :carrierSolution @06:13:20.59 delay 2 seconds @06:13:22.62 AS.reconfigure ASconfig @06:13:22.69 AS.seek 0.5ml @06:13:33.04 delay 1 second @06:13:34.06 AFV.select :holdingCoil @06:13:34.18 ATV.dial :air1 @06:13:36.23 AS.reconfigure ASslow1 @06:13:36.30 AS.seek 0.52ml @06:13:40.65 ATV.dial :ASV @06:13:42.55 ASV.dial :SPErecovery @06:13:43.28 AS.seek 0.62ml @06:14:03.68 ATV.dial :waste @06:14:06.44 AS.reconfigure ASnorm @06:14:06.51 AS.seek :empty @06:14:38.16 AFV.select :holdingCoil @06:14:38.17 ATV.dial :waste @06:14:38.21 AS.reconfigure ASconfig @06:14:38.29 AS.seek 0ml @06:14:38.61 AFV.select :carrierSolution @06:14:38.75 delay 2 seconds @06:14:40.79 AS.seek 0.5ml @06:15:09.83 delay 1 second @06:15:10.85 AFV.select :holdingCoil @06:15:10.97 ATV.dial :air3 @06:15:11.73 AS.reconfigure ASslow1 @06:15:11.80 AS.seek 0.52ml @06:15:16.15 ATV.dial :air3 @06:15:16.25 AS.seek 0.53ml @06:15:18.60 delay 2 seconds @06:15:20.62 ATV.dial :bleach @06:15:21.12 AS.seek 0.71ml @06:15:57.71 delay 3 seconds @06:16:00.74 ATV.dial :air3 @06:16:01.37 delay 3 seconds @06:16:04.46 AS.seek 0.735ml @06:16:09.82 delay 3 seconds @06:16:12.85 ATV.dial :bleach @06:16:13.53 AS.seek 0.72ml @06:16:16.88 delay 3 seconds @06:16:19.91 ATV.dial ASV @06:16:21.05 ASV.dial :SPErecovery @06:16:21.15 AS.seek 0.365ml @06:17:32.81 ATV.dial :waste @06:17:34.65 AS.reconfigure ASnorm @06:17:34.72 AS.seek :empty @06:17:53.52 ATV.dial :air3 @06:17:54.25 AS.reconfigure ASslow1 @06:17:54.32 AS.seek 0.009ml @06:17:56.66 delay 2 seconds @06:17:58.68 ATV.dial :bleach @06:17:59.15 AS.seek 0.579ml @06:19:54.10 delay 3 seconds @06:19:57.13 ATV.dial :air3 @06:19:57.71 delay 3 seconds @06:20:00.80 AS.seek 0.604ml @06:20:06.17 delay 3 seconds @06:20:09.20 ATV.dial :bleach @06:20:09.84 AS.seek 0.589ml @06:20:13.29 delay 3 seconds @06:20:16.32 ATV.dial :ASV @06:20:17.40 ASV.dial :wasteR @06:20:17.81 AS.seek 0ml @06:22:16.62 delay 1:00 @06:23:16.65 ATV.dial ASV @06:23:16.66 AFV.select :carrierSolution @06:23:16.79 delay 2 seconds @06:23:18.82 AS.reconfigure ASconfig @06:23:18.89 AS.seek 1ml @06:24:16.66 delay 1 second @06:24:17.68 AFV.select :holdingCoil @06:24:17.79 delay 1 second @06:24:18.82 ASV.dial :wasteR @06:24:18.84 AS.reconfigure ASslow1 @06:24:18.92 AS.seek 0ml @06:27:40.32 delay 10 seconds @06:27:50.35 ATV.dial :air3 @06:27:51.90 AS.seek 0.01ml @06:27:54.29 delay 2 seconds @06:27:56.31 ATV.dial :bleach @06:27:56.97 AS.seek 0.58ml @06:29:51.90 delay 3 seconds @06:29:54.93 ATV.dial :air3 @06:29:55.51 delay 3 seconds @06:29:58.60 AS.seek 0.605ml @06:30:03.95 delay 3 seconds @06:30:06.98 ATV.dial :bleach @06:30:07.64 AS.seek 0.59ml @06:30:10.99 delay 3 seconds @06:30:14.02 ATV.dial ASV @06:30:15.08 ASV.dial :SPEdelivery @06:30:15.49 AS.seek 0ml @06:32:14.44 delay 1:00 @06:33:14.47 AFV.select :carrierSolution @06:33:14.59 delay 2 seconds @06:33:16.62 AS.reconfigure ASconfig @06:33:16.69 AS.seek 1ml @06:34:14.49 delay 1 second @06:34:15.51 AFV.select :holdingCoil @06:34:15.63 delay 1 second @06:34:16.66 ATV.dial ASV @06:34:17.19 ASV.dial :SPEdelivery @06:34:17.32 AS.reconfigure ASslow1 @06:34:17.39 AS.seek 0ml @06:37:38.82 delay 10 seconds @06:37:48.85 ATV.dial :air2 @06:37:49.71 AS.reconfigure ASslow0 @06:37:49.78 AS.seek 0.8ml @06:39:10.60 delay 3 seconds @06:39:13.63 ATV.dial :ASV @06:39:14.66 ASV.dial :SPEdelivery @06:39:14.69 AS.reconfigure ASslow1 @06:39:14.76 AS.seek 0.03ml @06:41:49.93 delay 20 seconds @06:42:09.96 ATV.dial :waste,via: :air3 @06:42:11.74 AS.reconfigure ASconfig @06:42:11.81 AS.seek :empty @06:42:13.90 SPE.coast @06:42:14.01 Removing Bleach from Mixing Coil @06:42:14.02 AFV.select :carrierSolution @06:42:14.15 delay 2 seconds @06:42:16.19 AS.seek 0.5ml @06:42:45.23 delay 1 second @06:42:46.25 AFV.select :holdingCoil @06:42:46.37 ATV.dial :ASV @06:42:48.10 ASV.dial :SPErecovery @06:42:48.78 AS.reconfigure ASslow1 @06:42:48.85 AS.seek 0.73ml @06:43:35.48 ASV.dial :wasteD @06:43:36.09 AS.reconfigure ASnorm @06:43:36.16 AS.seek 0ml @06:44:13.29 AFV.select :carrierSolution @06:44:13.42 delay 2 seconds @06:44:15.45 AS.reconfigure ASconfig @06:44:15.52 AS.seek 0.5ml @06:44:44.60 delay 1 second @06:44:45.62 AFV.select :holdingCoil @06:44:45.74 ATV.dial ASV @06:44:45.76 ASV.dial :SPErecovery @06:44:46.44 AS.reconfigure ASslow0 @06:44:46.51 AS.seek 0.3ml @06:45:06.98 ATV.dial :air2 @06:45:08.06 AS.reconfigure ASslow1 @06:45:08.13 AS.seek 0.32ml @06:45:12.46 ATV.dial ASV @06:45:13.55 AS.reconfigure ASslow0 @06:45:13.62 AS.seek 0.57ml @06:45:39.13 ASV.dial :wasteD @06:45:39.74 AS.reconfigure ASnorm @06:45:39.82 AS.seek 0ml @06:46:08.87 AFV.select :carrierSolution @06:46:09.01 delay 2 seconds @06:46:11.04 AS.reconfigure ASconfig @06:46:11.11 AS.seek 0.5ml @06:46:40.19 delay 1 second @06:46:41.21 AFV.select :holdingCoil @06:46:41.33 ATV.dial ASV @06:46:41.35 ASV.dial :SPErecovery @06:46:42.01 AS.reconfigure ASslow0 @06:46:42.08 AS.seek 0.3ml @06:47:02.54 ATV.dial :air2 @06:47:03.62 AS.reconfigure ASslow1 @06:47:03.69 AS.seek 0.32ml @06:47:08.02 ATV.dial ASV @06:47:09.11 AS.reconfigure ASslow0 @06:47:09.18 AS.seek 0.57ml @06:47:34.68 ASV.dial :wasteD @06:47:35.29 AS.reconfigure ASnorm @06:47:35.37 AS.seek 0ml @06:48:04.46 ATV.dial :air2 @06:48:05.48 AS.reconfigure ASslow0 @06:48:05.56 AS.seek 0.3ml @06:48:36.10 ATV.dial :ASV @06:48:37.12 ASV.dial :wasteD @06:48:37.14 AS.reconfigure ASconfig @06:48:37.22 AS.seek 0.05ml @06:48:51.93 ATV.dial :waste @06:48:53.85 AS.seek 0ml @06:48:57.08 ASV.dial :SPEdelivery @06:48:58.18 deconLysateINT @06:48:58.19 ATV.dial :air3 @06:48:58.91 AS.reconfigure ASslow1 @06:48:58.98 AS.seek 0.01ml @06:49:01.30 delay 2 seconds @06:49:03.32 ATV.dial :bleach @06:49:03.80 AS.seek 0.96ml @06:52:15.18 delay 3 seconds @06:52:18.21 ATV.dial :air3 @06:52:18.79 delay 3 seconds @06:52:21.88 AS.seek 0.985ml @06:52:27.32 delay 3 seconds @06:52:30.35 ATV.dial :bleach @06:52:30.98 AS.seek 0.97ml @06:52:34.26 delay 3 seconds @06:52:37.29 ATV.dial AIV @06:52:38.57 AIV.dial :coilToMFB @06:52:38.59 AWV.select :waste @06:52:38.61 AFV.select :carrierSolution @06:52:38.75 delay 2 seconds @06:52:40.78 AS.reconfigure ASconfig @06:52:40.88 AS.seek 1ml @06:52:42.96 delay 1 second @06:52:43.98 AFV.select :holdingCoil @06:52:44.10 delay 1 second @06:52:45.13 AS.reconfigure ASslow0 @06:52:45.20 AS.seek :empty @06:54:26.21 AFV.select :carrierSolution @06:54:26.34 delay 2 seconds @06:54:28.37 AS.reconfigure ASconfig @06:54:28.45 AS.seek 1ml @06:55:26.26 delay 1 second @06:55:27.28 AFV.select :holdingCoil @06:55:27.40 delay 1 second @06:55:28.43 AS.reconfigure ASslow0 @06:55:28.50 AS.seek :empty @06:57:09.45 AFV.select :carrierSolution @06:57:09.59 delay 2 seconds @06:57:11.62 AS.reconfigure ASconfig @06:57:11.69 AS.seek 1ml @06:58:09.54 delay 1 second @06:58:10.56 AFV.select :holdingCoil @06:58:10.68 delay 1 second @06:58:11.71 AS.reconfigure ASslow0 @06:58:11.78 AS.seek :empty @06:59:52.79 ATV.dial :air2 @06:59:53.50 AS.reconfigure ASslow1 @06:59:53.57 AS.seek 0.009ml @06:59:55.90 AS.reconfigure ASslow0 @06:59:55.98 AS.seek 1ml @07:01:35.94 ATV.dial AIV @07:01:36.77 AS.seek 0.02ml @07:03:15.67 ATV.dial :waste @07:03:17.38 AS.seek 0ml @07:03:19.68 AWV.select :air @07:03:19.87 Cleaning PCR @07:03:19.88 AFV.select :carrierSolution @07:03:20.02 delay 2 seconds @07:03:22.05 AS.reconfigure ASconfig @07:03:22.12 AS.seek 0.5ml @07:03:51.18 delay 1 second @07:03:52.20 AFV.select :holdingCoil @07:03:52.32 ASV.dial :waste1 @07:03:52.73 ATV.dial :air3 @07:03:53.44 AS.reconfigure ASslow1 @07:03:53.51 AS.seek 0.51ml @07:03:55.82 delay 2 seconds @07:03:57.84 ATV.dial :bleach @07:03:58.33 AS.seek 0.81ml @07:04:58.96 delay 3 seconds @07:05:01.99 ATV.dial :air3 @07:05:02.57 delay 3 seconds @07:05:05.66 AS.seek 0.835ml @07:05:11.11 delay 3 seconds @07:05:14.14 ATV.dial :bleach @07:05:14.80 AS.seek 0.82ml @07:05:18.12 delay 3 seconds @07:05:21.15 ARV.dial :waste @07:05:21.77 delay 3 seconds @07:05:24.80 ATV.dial :ARV @07:05:25.69 AS.reconfigure ASconfig @07:05:25.77 AS.seek 0.47ml @07:05:46.21 AFV.select :carrierSolution @07:05:46.34 delay 2 seconds @07:05:48.38 AS.seek 1ml @07:06:19.16 delay 1 second @07:06:20.18 AFV.select :holdingCoil @07:06:20.30 delay 1 second @07:06:21.33 AS.reconfigure ASfast @07:06:21.40 AS.seek 0ml @07:06:48.77 AFV.select :carrierSolution @07:06:48.89 delay 2 seconds @07:06:50.92 AS.reconfigure ASconfig @07:06:50.99 AS.seek 1ml @07:07:48.79 delay 1 second @07:07:49.81 AFV.select :holdingCoil @07:07:49.93 delay 1 second @07:07:50.96 AS.reconfigure ASfast @07:07:51.03 AS.seek 0ml @07:08:18.39 AFV.select :carrierSolution @07:08:18.53 delay 2 seconds @07:08:20.56 AS.reconfigure ASconfig @07:08:20.63 AS.seek 1ml @07:09:18.40 delay 1 second @07:09:19.42 AFV.select :holdingCoil @07:09:19.54 delay 1 second @07:09:20.57 AS.reconfigure ASfast @07:09:20.64 AS.seek 0ml @07:09:48.02 ARV.dial between carrier and air1 @07:09:49.12 ATV.dial :air3 @07:09:50.44 AS.reconfigure ASslow1 @07:09:50.51 AS.seek 0.01ml @07:09:52.88 delay 2 seconds @07:09:54.90 ATV.dial :bleach @07:09:55.55 AS.seek 0.81ml @07:12:36.71 delay 3 seconds @07:12:39.74 ATV.dial :air3 @07:12:40.32 delay 3 seconds @07:12:43.41 AS.seek 0.835ml @07:12:48.71 delay 3 seconds @07:12:51.74 ATV.dial :bleach @07:12:52.39 AS.seek 0.82ml @07:12:55.73 delay 3 seconds @07:12:58.76 ATV.dial PCR @07:12:59.18 AS.reconfigure ASconfig @07:12:59.25 AS.seek :empty @07:13:46.73 PCR.program :bleachProfile @07:13:46.80 PCR program should complete in < 2:45 @07:13:46.81 PCR.run 10:00 @07:16:48.66 PCR.off @07:16:48.70 AFV.select :carrierSolution @07:16:48.82 delay 2 seconds @07:16:50.86 AS.seek 1ml @07:17:48.68 delay 1 second @07:17:49.70 AFV.select :holdingCoil @07:17:49.82 delay 1 second @07:17:50.85 AS.reconfigure ASfast @07:17:50.92 AS.seek 0ml @07:18:18.27 delay 3 seconds @07:18:21.30 AFV.select :carrierSolution @07:18:21.42 delay 2 seconds @07:18:23.45 AS.reconfigure ASconfig @07:18:23.53 AS.seek 1ml @07:19:21.34 delay 1 second @07:19:22.36 AFV.select :holdingCoil @07:19:22.48 delay 1 second @07:19:23.51 AS.reconfigure ASfast @07:19:23.58 AS.seek 0ml @07:19:50.94 delay 3 seconds @07:19:53.97 AFV.select :carrierSolution @07:19:54.09 delay 2 seconds @07:19:56.12 AS.reconfigure ASconfig @07:19:56.19 AS.seek 1ml @07:20:54.01 delay 1 second @07:20:55.03 AFV.select :holdingCoil @07:20:55.15 delay 1 second @07:20:56.18 AS.reconfigure ASfast @07:20:56.25 AS.seek 0ml @07:21:23.62 delay 3 seconds @07:21:26.65 AFV.select :carrierSolution @07:21:26.77 delay 2 seconds @07:21:28.80 AS.reconfigure ASconfig @07:21:28.88 AS.seek 1ml @07:22:26.65 delay 1 second @07:22:27.67 AFV.select :holdingCoil @07:22:27.79 delay 1 second @07:22:28.82 AS.reconfigure ASfast @07:22:28.89 AS.seek 0ml @07:22:56.26 delay 3 seconds @07:22:59.29 AFV.select :carrierSolution @07:22:59.41 delay 2 seconds @07:23:01.44 AS.reconfigure ASconfig @07:23:01.51 AS.seek 1ml @07:23:59.30 delay 1 second @07:24:00.32 AFV.select :holdingCoil @07:24:00.44 delay 1 second @07:24:01.47 AS.reconfigure ASnorm @07:24:01.54 AS.seek 0ml @07:24:52.25 AFV.select :carrierSolution @07:24:52.38 delay 2 seconds @07:24:54.41 AS.reconfigure ASconfig @07:24:54.48 AS.seek 1ml @07:25:52.29 delay 1 second @07:25:53.31 AFV.select :holdingCoil @07:25:53.42 delay 1 second @07:25:54.45 ATV.dial :waste @07:25:55.71 AS.reconfigure ASnorm @07:25:55.79 AS.seek 0.5ml @07:26:21.37 ATV.dial :waste2 @07:26:22.70 AS.seek 0ml @07:26:48.29
Asleep after completing 20invasivedeploymfbtest assays @07:26:48.31 Mission runtime was 15:04:54.906934 @07:26:48.34 GoodNews.email "{1 => 9, 2 => 0}\nCan@07:21:53, 21.3C, 28% humidity, 14.7psia, 11.987V, 0.594A, 0.594A avg, 7.12W",Subject: "Asleep after completing 20invasivedeploymfbtest assays" @07:26:57.66 Session Ended @16:14:08.90CEST06-May-20
ESP2 v3.30 I2C Gateway v3.06 for core bus @16:14:11.76 Copyright 2018 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @16:14:12.24 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @16:14:19.34 Polling Can every 10:00 @16:14:21.18 SPE.coast @16:14:21.24 SPE.configure SPEconfig @16:14:21.33 AS.configure ASconfig @16:14:21.43 SS.reconfigure SSconfig @16:14:21.50 SS.hold @16:14:21.62 SS.configure SSconfig @16:14:21.81 CC.reconfigure CCconfig @16:14:21.90 CC.hold @16:14:22.01 CC.configure CCconfig @16:14:22.20 CS.reconfigure CSconfig @16:14:22.29 CS.hold @16:14:22.40 CS.configure CSconfig @16:14:22.49 PC.reconfigure PCconfig @16:14:22.54 PC.hold @16:14:22.97 PC.configure PCconfig @16:14:23.06 PS.configure PSconfig @16:14:23.18 Forearm.reconfigure LooseForearm @16:14:23.22 Forearm.hold @16:14:23.28 Forearm.configure LooseForearm @16:14:23.41 Elbow.reconfigure LooseElbow @16:14:23.50 Elbow.hold @16:14:23.61 Elbow.configure LooseElbow @16:14:23.84 SC.reconfigure SCloose @16:14:23.92 SC.hold @16:14:24.04 SC.configure SCloose @16:14:24.23 SE.reconfigure SEconfig @16:14:24.29 SE.hold @16:14:24.36 SE.configure SEconfig @16:14:24.40 CH.coast @16:14:24.45 CH.configure CHconfig @16:14:24.48 PH.coast @16:14:24.54 PH.configure PHconfig @16:14:24.58 PCR.off @16:14:24.61 PCR.pidtemp= 50,0.1,0,300,25,25 @16:14:24.65 PCR.caltemp= 2.8814,0.0372,0 @16:14:24.68 All dwarf controllers running firmware version 3.75 @16:14:24.79 Cleanly exited at 16:13:35CEST06-May-20 @16:14:24.83 Loading /home/esp/esp2/mission/20invasivedeploywcrtest.rb mission @16:14:24.93 Preparing to sleep until +0:00:05 @16:14:24.97 GoodNews.email "Starting 20invasivedeploywcrtest w/pucks in tube 2\n{}\nCan@16:14:19, 21.3C, 30% humidity, 14.7psia, 11.972V, 0.550A, 0.550A avg, 6.59W",Subject: "20invasivedeploywcrtest begun" @16:14:25.17 SS.coast @16:14:25.24
Asleep til +0:00:05 @16:14:25.33 Continuing at 16:14 @16:14:25.37 CC.coast @16:14:25.59 CS.coast @16:14:25.69 PC.coast @16:14:25.77 Forearm.coast @16:14:26.04 Elbow.coast @16:14:26.11 Session Begun @16:14:26.20 SC.coast @16:14:26.31 SE.coast @16:14:26.82 Gate.power :core,:OFF @16:14:26.86 Gate.power :raw,:OFF @16:14:26.90 Shutdown @16:14:29.96
Powering core bus @16:14:33.02 GoodNews.email "Then, in tube 2: wcrphase\n/home/esp/esp2/mission/20invasivedeploywcrtest.rb:119\n{}\nCan@16:14:25, 21.3C, 30% humidity, 14.7psia, 11.972V, 0.544A, 0.544A avg, 6.51W",Subject: "Asleep until 16:14" @16:14:35.26
Gate.power :raw,:ON @16:14:37.82 SPE.coast @16:14:38.06 SPE.configure SPEconfig @16:14:38.37 AS.configure ASconfig @16:14:38.68 SS.configure SSconfig @16:14:38.99 CC.configure CCconfig @16:14:39.48 CS.configure CSconfig @16:14:39.90 PC.configure PCconfig @16:14:40.18 PS.configure PSconfig @16:14:40.57 Forearm.configure LooseForearm @16:14:40.90 Elbow.configure LooseElbow @16:14:41.33 SC.configure SCloose @16:14:41.74 SE.configure SEconfig @16:14:41.93 CH.coast @16:14:42.21 CH.configure CHconfig @16:14:42.37 PH.coast @16:14:42.61 PH.configure PHconfig @16:14:42.79 PCR.off @16:14:42.91 PCR.pidtemp= 50,0.1,0,300,25,25 @16:14:43.05 PCR.caltemp= 2.8814,0.0372,0 @16:14:43.20 All dwarf controllers running firmware version 3.75 @16:14:43.54 Valve.reset @16:14:44.05 PCR.off @16:14:44.18 PCR.pidtemp= 50,0.1,0,300,25,25 @16:14:44.30 PCR.caltemp= 2.8814,0.0372,0 @16:14:44.82 Intake.select :close @16:14:44.94 Exhaust.select :close @16:14:45.12 CTV.dial :air @16:14:45.52 PSV.dial :air @16:14:45.60 CSV.dial :waste1 @16:14:46.28 SSV.dial :spit,avoiding: :exhaust @16:14:46.42 PS.reconfigure PSconfig @16:14:46.49
Forearm.reconfigure LooseForearm @16:14:47.04 PS.home.seek 0ml @16:14:47.12
Forearm.home.seek :retract @16:14:49.13 CS.reconfigure CSfast @16:14:49.25 SS.reconfigure SSconfig @16:14:49.59 CS.home.seek 0ml @16:14:49.80 SS.home.seek 0ml @16:14:50.19 Hand.close @16:14:50.32
Elbow.jog 1500 @16:14:51.03 Elbow.reconfigure LooseElbow @16:14:51.24 Elbow.home.seek :clear @16:14:56.32 SE.reconfigure SEconfig @16:14:56.52 SE.home.seek :down @16:14:57.41 SC.reconfigure SCsloppy @16:14:57.62 SC.home.seek :initial @16:14:57.98 PS.coast @16:15:08.07
Forearm.seek :retract @16:15:08.39 Elbow.seek :hammer @16:15:09.74 Forearm.reconfigure TightForearm @16:15:09.95 Forearm.seek 5 + 350 counts @16:15:11.43 Forearm.seek :retract @16:15:13.33 PC.reconfigure PCconfig @16:15:13.42
CC.reconfigure CCconfig @16:15:13.74 PC.home.seek :open @16:15:13.82
CC.home.seek :open @16:15:18.32 Phase [wcrphase] @16:15:18.37 GoodNews.email "At 16:15:18 began in tube 2: wcrphase\n/home/esp/esp2/mission/20invasivedeploywcrtest.rb:119\n{}\nCan@16:15:18, 21.3C, 30% humidity, 14.7psia, 11.893V, 0.777A, 0.777A avg, 9.24W",Subject: "Started 20invasivedeploywcrtest phase wcrphase" @16:15:18.48CEST06-May-20
===> STARTED WCRSphase (phase #1) <=== Everybody has a plan untill they get punched in the face - Mike Tyson @16:15:18.57 Starting stand-alone bac WCR @16:15:18.64 bac wcr sampling at most 1500ml @16:15:18.77 Whole Cell Archive sampling 1500ml @16:15:18.87 Loading wcr puck from tube 2 into Collection Clamp @16:15:19.19 Hand.open @16:15:20.83 SC.reconfigure SCloose @16:15:21.48 Elbow.reconfigure TightElbow @16:15:21.72 Elbow.seek 2 @16:15:22.64 Forearm.seek 2 + 700 counts @16:15:24.02 SE.reconfigure SEpick @16:15:24.32 SE.seek beamBreak @16:15:27.88 SE.configure SEconfig @16:15:28.62 Elbow.seek 2 @16:15:29.53 Forearm.seek 2 + 300 counts @16:15:29.96 Forearm.reconfigure LooseForearm @16:15:30.28 Hand.close @16:15:30.71 BACfans.email "Started stand-alone bac WCR in tube 2 at 16:15:18CEST06-May-20\n{}\nCan@16:15:18, 21.3C, 30% humidity, 14.7psia, 11.893V, 0.777A, 0.777A avg, 9.24W",Subject: "bac wcr sampling at most 1500ml" @16:15:31.37 SE.seek :down @16:15:32.73 Forearm.seek :retract @16:15:34.00 Elbow.seek CC @16:15:35.06 SE.coast @16:15:36.44 Elbow.reconfigure LooseElbow @16:15:37.26 Forearm.reconfigure TightForearm @16:15:37.74 Forearm.seek CC @16:15:40.79 CC.seek :closed @16:15:47.31 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.45 @16:15:47.36 Hand.open @16:15:47.75 I2C::UnexpectedReply in core -- I2C::Servo::Status3Reply[69:27->20] @16:15:48.52 Forearm.reconfigure LooseForearm @16:15:48.80 Forearm.seek :retract @16:15:50.04 SC.coast @16:15:50.30 collectSample 1500ml w/bubble point of 55psi @16:15:50.39 Priming 7ml @16:15:50.48 Intake.select :close @16:15:50.60 Exhaust.select :close @16:15:50.83 SS.hold @16:15:51.89 Calibrating pressure and temperature sensors @16:15:52.00 CTV.dial :air @16:15:52.09 SSV.dial :spit,avoiding: :exhaust @16:15:52.26 SS.reconfigure SSfast @16:15:52.60 SS.seek 5ml @16:15:55.99 SSV.dial CBV @16:15:56.14 CBV.dial SSV @16:15:57.99 delay 3.8 seconds @16:16:02.09 SSV.dial :spit @16:16:03.77 SS.seek 0ml @16:16:06.57 SSV.dial CBV @16:16:08.26 CTV.dial :intake @16:16:09.35 SSV.dial CBV @16:16:09.44 CBV.dial SSV @16:16:09.56 Allowing 40s for 7.0ml of remaining 7.0ml @16:16:09.63 Intake.select :open @16:16:12.11 Intake @15.6psia, vacuum from 9.1 to 13.1psi @16:16:12.19 SS.reconfigure SSinhale.with maxSpeed: 150 @16:16:12.41 SS.seek 7ml @16:16:19.14 Stroke completed -- waiting for pressure to equalize ... @16:16:36.69 Intake.select :close @16:16:37.09 SSV.dial :exhaust @16:16:38.10 Exhaust.select :open @16:16:38.35 SS.reconfigure SSexhale @16:16:38.54 SS.seek 0ml @16:16:43.00 Exhaust.select :close @16:16:43.20 Sampling 1500.0ml @16:16:43.23 Intake.select :close @16:16:43.27 Exhaust.select :close @16:16:43.35 SS.reconfigure SSconfig @16:16:43.49 SS.hold @16:16:43.94 CTV.dial :intake @16:16:43.99 SSV.dial CBV @16:16:44.10 CBV.dial SSV @16:16:45.06 Allowing 130s for 25.0ml of remaining 1500.0ml @16:16:45.10 Intake.select :open @16:16:47.42 Intake @15.5psia, vacuum from 9.0 to 13.0psi @16:16:47.46 SS.reconfigure SSinhale.with maxSpeed: 150 @16:16:47.64 SS.seek 25ml @16:16:52.23 Vacuum too high -- waiting up to 40s @16:17:01.24 Intake @15.6psia, vacuum from 9.1 to 13.1psi @16:17:01.26 SS.reconfigure SSinhale.with maxSpeed: 135 @16:17:01.35 SS.seek 25ml @16:17:05.23 Vacuum too high -- waiting up to 40s @16:17:14.56 Intake @15.6psia, vacuum from 9.1 to 13.1psi @16:17:14.59 SS.reconfigure SSinhale.with maxSpeed: 122 @16:17:14.68 SS.seek 25ml @16:17:18.83 Vacuum too high -- waiting up to 40s @16:17:28.92 Intake @15.7psia, vacuum from 9.2 to 13.2psi @16:17:28.95 SS.reconfigure SSinhale.with maxSpeed: 110 @16:17:29.04 SS.seek 25ml @16:17:33.44 Vacuum too high -- waiting up to 40s @16:17:43.54 Intake @15.7psia, vacuum from 9.2 to 13.2psi @16:17:43.57 SS.reconfigure SSinhale.with maxSpeed: 99 @16:17:43.65 SS.seek 25ml @16:17:48.20 Stroke completed -- waiting for pressure to equalize ... @16:18:02.01 Intake.select :close @16:18:02.19 SSV.dial :exhaust @16:18:03.09 Exhaust.select :open @16:18:03.22 SS.reconfigure SSexhale @16:18:03.30 SS.seek 0ml @16:18:12.91 Exhaust.select :close @16:18:13.05 SSV.dial CBV @16:18:13.09 CBV.dial SSV @16:18:14.00 Allowing 130s for 25.0ml of remaining 1475.0ml @16:18:14.02 Intake.select :open @16:18:16.71 Intake @15.6psia, vacuum from 9.1 to 13.1psi @16:18:16.73 SS.reconfigure SSinhale.with maxSpeed: 99 @16:18:16.82 SS.seek 25ml @16:18:20.76 Vacuum too high -- waiting up to 40s @16:18:26.87 Intake @15.6psia, vacuum from 9.1 to 13.1psi @16:18:26.90 SS.reconfigure SSinhale.with maxSpeed: 89 @16:18:26.99 SS.seek 25ml @16:18:30.92 Vacuum too high -- waiting up to 40s @16:18:37.75 Intake @15.6psia, vacuum from 9.1 to 13.1psi @16:18:37.77 SS.reconfigure SSinhale.with maxSpeed: 80 @16:18:37.86 SS.seek 25ml @16:18:42.48 Vacuum too high -- waiting up to 40s @16:18:50.10 Intake @15.6psia, vacuum from 9.1 to 13.1psi @16:18:50.13 SS.reconfigure SSinhale.with maxSpeed: 72 @16:18:50.21 SS.seek 25ml @16:18:55.16 Vacuum too high -- waiting up to 40s @16:19:03.68 Intake @15.6psia, vacuum from 9.1 to 13.1psi @16:19:03.71 SS.reconfigure SSinhale.with maxSpeed: 65 @16:19:03.80 SS.seek 25ml @16:19:10.02 Vacuum too high -- waiting up to 40s @16:19:18.12 SS.reconfigure SSinhale.with maxSpeed: 59 @16:19:18.19 SS.seek 25ml @16:19:23.14 Stroke completed -- waiting for pressure to equalize ... @16:19:33.22 Intake.select :close @16:19:33.40 SSV.dial :exhaust @16:19:34.31 Exhaust.select :open @16:19:34.44 SS.reconfigure SSexhale @16:19:34.51 SS.seek 0ml @16:19:44.19 Exhaust.select :close @16:19:44.33 SSV.dial CBV @16:19:44.37 CBV.dial SSV @16:19:45.28 Allowing 130s for 25.0ml of remaining 1450.0ml @16:19:45.30 Intake.select :open @16:19:47.50 Intake @15.7psia, vacuum from 9.2 to 13.2psi @16:19:47.92 SS.reconfigure SSinhale.with maxSpeed: 59 @16:19:48.09 SS.seek 25ml @16:19:53.61 Vacuum too high -- waiting up to 40s @16:19:58.79 Intake @15.5psia, vacuum from 9.0 to 13.0psi @16:19:58.82 SS.reconfigure SSinhale.with maxSpeed: 53 @16:19:58.91 SS.seek 25ml @16:20:04.97 Vacuum too high -- waiting up to 40s @16:20:10.81 Intake @15.5psia, vacuum from 9.0 to 13.0psi @16:20:10.83 SS.reconfigure SSinhale.with maxSpeed: 48 @16:20:10.92 SS.seek 25ml @16:20:19.04 Vacuum too high -- waiting up to 40s @16:20:26.14 Intake @15.6psia, vacuum from 9.1 to 13.1psi @16:20:26.16 SS.reconfigure SSinhale.with maxSpeed: 43 @16:20:26.25 SS.seek 25ml @16:20:37.54 Vacuum too high -- waiting up to 40s @16:20:45.63 Intake @15.6psia, vacuum from 9.1 to 13.1psi @16:20:45.66 SS.reconfigure SSinhale.with maxSpeed: 39 @16:20:45.75 SS.seek 25ml @16:20:55.69 Stroke completed -- waiting for pressure to equalize ... @16:21:06.88 Intake.select :close @16:21:07.07 SSV.dial :exhaust @16:21:07.96 Exhaust.select :open @16:21:08.09 SS.reconfigure SSexhale @16:21:08.16 SS.seek 0ml @16:21:17.80 Exhaust.select :close @16:21:17.94 SSV.dial CBV @16:21:17.97 CBV.dial SSV @16:21:18.89 Allowing 130s for 25.0ml of remaining 1425.0ml @16:21:18.91 Intake.select :open @16:21:21.11 Intake @15.7psia, vacuum from 9.2 to 13.2psi @16:21:21.14 SS.reconfigure SSinhale.with maxSpeed: 39 @16:21:21.22 SS.seek 25ml @16:21:32.82 Vacuum too high -- waiting up to 40s @16:21:38.91 Intake @15.5psia, vacuum from 9.0 to 13.0psi @16:21:38.93 SS.reconfigure SSinhale.with maxSpeed: 35 @16:21:39.02 SS.seek 25ml @16:21:55.10 Vacuum too high -- waiting up to 40s @16:22:02.71 SS.reconfigure SSinhale.with maxSpeed: 32 @16:22:02.78 SS.seek 25ml @16:22:27.41 Vacuum too high -- waiting up to 40s @16:22:37.01 SS.reconfigure SSinhale.with maxSpeed: 29 @16:22:37.09 SS.seek 25ml @16:22:38.44 Stroke completed -- waiting for pressure to equalize ... @16:22:43.39 Intake.select :close @16:22:43.58 SSV.dial :exhaust @16:22:44.49 Exhaust.select :open @16:22:44.62 SS.reconfigure SSexhale @16:22:44.70 SS.seek 0ml @16:22:54.39 Exhaust.select :close @16:22:54.53 SSV.dial CBV @16:22:54.56 CBV.dial SSV @16:22:55.49 Allowing 130s for 25.0ml of remaining 1400.0ml @16:22:55.51 Intake.select :open @16:22:57.72 Intake @15.6psia, vacuum from 9.1 to 13.1psi @16:22:57.75 SS.reconfigure SSinhale.with maxSpeed: 29 @16:22:57.84 SS.seek 25ml @16:23:20.00 Vacuum too high -- waiting up to 40s @16:23:25.88 Intake @15.4psia, vacuum from 8.9 to 12.9psi @16:23:25.91 SS.reconfigure SSinhale.with maxSpeed: 26 @16:23:26.00 SS.seek 25ml @16:24:09.39 Stroke completed -- waiting for pressure to equalize ... @16:24:24.25 Intake.select :close @16:24:24.44 SSV.dial :exhaust @16:24:25.35 Exhaust.select :open @16:24:25.49 SS.reconfigure SSexhale @16:24:25.56 SS.seek 0ml @16:24:35.26 Exhaust.select :close @16:24:35.40 SSV.dial CBV @16:24:35.44 CBV.dial SSV @16:24:36.37 Allowing 130s for 25.0ml of remaining 1375.0ml @16:24:36.39 Intake.select :open @16:24:38.59 Intake @15.6psia, vacuum from 9.1 to 13.1psi @16:24:38.62 SS.reconfigure SSinhale.with maxSpeed: 26 @16:24:38.71 SS.seek 25ml @16:25:13.13 Vacuum too high -- waiting up to 40s @16:25:20.80 Intake @15.4psia, vacuum from 8.9 to 12.9psi @16:25:20.83 SS.reconfigure SSinhale.with maxSpeed: 23 @16:25:20.92 SS.seek 25ml @16:25:59.67 Stroke completed -- waiting for pressure to equalize ... @16:26:13.33 Intake.select :close @16:26:13.51 SSV.dial :exhaust @16:26:14.41 Exhaust.select :open @16:26:14.54 SS.reconfigure SSexhale @16:26:14.62 SS.seek 0ml @16:26:24.42 Exhaust.select :close @16:26:24.56 SSV.dial CBV @16:26:24.59 CBV.dial SSV @16:26:25.51 Allowing 130s for 25.0ml of remaining 1350.0ml @16:26:25.53 Intake.select :open @16:26:27.74 Intake @15.6psia, vacuum from 9.1 to 13.1psi @16:26:27.76 SS.reconfigure SSinhale.with maxSpeed: 23 @16:26:27.85 SS.seek 25ml @16:27:22.35 Vacuum too high -- waiting up to 40s @16:27:30.94 Intake @15.4psia, vacuum from 8.9 to 12.9psi @16:27:30.96 SS.reconfigure SSinhale.with maxSpeed: 21 @16:27:31.05 SS.seek 25ml @16:27:55.78 Stroke completed -- waiting for pressure to equalize ... @16:28:07.61 Intake.select :close @16:28:07.80 SSV.dial :exhaust @16:28:08.71 Exhaust.select :open @16:28:08.84 SS.reconfigure SSexhale @16:28:08.91 SS.seek 0ml @16:28:18.25 Exhaust.select :close @16:28:18.39 SSV.dial CBV @16:28:18.42 CBV.dial SSV @16:28:19.35 Allowing 130s for 25.0ml of remaining 1325.0ml @16:28:19.37 Intake.select :open @16:28:21.59 Intake @15.6psia, vacuum from 9.1 to 13.1psi @16:28:21.61 SS.reconfigure SSinhale.with maxSpeed: 21 @16:28:21.70 SS.seek 25ml @16:29:46.06 Stroke completed -- waiting for pressure to equalize ... @16:30:03.63 Intake.select :close @16:30:03.81 SSV.dial :exhaust @16:30:04.69 Exhaust.select :open @16:30:04.82 SS.reconfigure SSexhale @16:30:04.89 SS.seek 0ml @16:30:14.76 Exhaust.select :close @16:30:14.90 SSV.dial CBV @16:30:14.93 CBV.dial SSV @16:30:15.85 Allowing 130s for 25.0ml of remaining 1300.0ml @16:30:15.87 Intake.select :open @16:30:18.07 Intake @15.6psia, vacuum from 9.1 to 13.1psi @16:30:18.10 SS.reconfigure SSinhale.with maxSpeed: 21 @16:30:18.18 SS.seek 25ml @16:31:29.41 Vacuum too high -- waiting up to 40s @16:31:40.02 Intake @15.5psia, vacuum from 9.0 to 13.0psi @16:31:40.05 SS.reconfigure SSinhale.with maxSpeed: 19 @16:31:40.13 SS.seek 25ml @16:31:54.65 Stroke completed -- waiting for pressure to equalize ... @16:32:04.67 Intake.select :close @16:32:04.85 SSV.dial :exhaust @16:32:05.76 Exhaust.select :open @16:32:05.89 SS.reconfigure SSexhale @16:32:05.96 SS.seek 0ml @16:32:15.65 Exhaust.select :close @16:32:15.78 SSV.dial CBV @16:32:15.81 CBV.dial SSV @16:32:16.76 Allowing 130s for 25.0ml of remaining 1275.0ml @16:32:16.78 Intake.select :open @16:32:17.81 Session Begun @16:32:19.01 Intake @15.6psia, vacuum from 9.1 to 13.1psi @16:32:19.04 SS.reconfigure SSinhale.with maxSpeed: 19 @16:32:19.15 SS.seek 25ml @16:32:20.83 -> can @16:32:42.88 -> PRV.legend @16:33:52.21 Stroke completed -- waiting for pressure to equalize ... @16:34:10.35 Intake.select :close @16:34:10.55 SSV.dial :exhaust @16:34:11.46 Exhaust.select :open @16:34:11.61 SS.reconfigure SSexhale @16:34:11.70 SS.seek 0ml @16:34:21.09 Exhaust.select :close @16:34:21.25 SSV.dial CBV @16:34:21.29 CBV.dial SSV @16:34:22.23 Allowing 130s for 25.0ml of remaining 1250.0ml @16:34:22.25 Intake.select :open @16:34:24.48 Intake @15.6psia, vacuum from 9.1 to 13.1psi @16:34:24.54 SS.reconfigure SSinhale.with maxSpeed: 19 @16:34:24.63 SS.seek 25ml @16:35:58.07 Stroke completed -- waiting for pressure to equalize ... @16:36:17.62 Intake.select :close @16:36:17.83 SSV.dial :exhaust @16:36:18.76 Exhaust.select :open @16:36:18.91 SS.reconfigure SSexhale @16:36:19.00 SS.seek 0ml @16:36:28.71 Exhaust.select :close @16:36:28.88 SSV.dial CBV @16:36:28.91 CBV.dial SSV @16:36:29.85 Allowing 130s for 25.0ml of remaining 1225.0ml @16:36:29.89 Intake.select :open @16:36:32.11 Intake @15.6psia, vacuum from 9.1 to 13.1psi @16:36:32.13 SS.reconfigure SSinhale.with maxSpeed: 19 @16:36:32.25 SS.seek 25ml @16:37:41.16 Vacuum too high -- waiting up to 40s @16:37:51.76 Intake @15.4psia, vacuum from 8.9 to 12.9psi @16:37:51.79 SS.reconfigure SSinhale.with maxSpeed: 17 @16:37:51.90 SS.seek 25ml @16:38:20.28 Stroke completed -- waiting for pressure to equalize ... @16:38:32.78 Intake.select :close @16:38:32.98 SSV.dial :exhaust @16:38:33.90 Exhaust.select :open @16:38:34.05 SS.reconfigure SSexhale @16:38:34.13 SS.seek 0ml @16:38:43.93 Exhaust.select :close @16:38:44.09 SSV.dial CBV @16:38:44.13 CBV.dial SSV @16:38:45.05 Allowing 130s for 25.0ml of remaining 1200.0ml @16:38:45.09 Intake.select :open @16:38:47.30 Intake @15.6psia, vacuum from 9.1 to 13.1psi @16:38:47.33 SS.reconfigure SSinhale.with maxSpeed: 17 @16:38:47.43 SS.seek 25ml @16:40:31.84 Stroke completed -- waiting for pressure to equalize ... @16:40:50.27 Intake.select :close @16:40:50.47 SSV.dial :exhaust @16:40:51.36 Exhaust.select :open @16:40:51.53 SS.reconfigure SSexhale @16:40:51.62 SS.seek 0ml @16:41:01.42 Exhaust.select :close @16:41:01.58 SSV.dial CBV @16:41:02.02 CBV.dial SSV @16:41:03.04 Allowing 130s for 25.0ml of remaining 1175.0ml @16:41:03.07 Intake.select :open @16:41:05.30 Intake @15.6psia, vacuum from 9.1 to 13.1psi @16:41:05.33 SS.reconfigure SSinhale.with maxSpeed: 17 @16:41:05.43 SS.seek 25ml @16:42:50.07 Stroke completed -- waiting for pressure to equalize ... @16:43:10.24 Intake.select :close @16:43:10.45 SSV.dial :exhaust @16:43:11.35 Exhaust.select :open @16:43:11.50 SS.reconfigure SSexhale @16:43:11.58 SS.seek 0ml @16:43:21.40 Exhaust.select :close @16:43:21.56 SSV.dial CBV @16:43:21.62 CBV.dial SSV @16:43:22.46 Allowing 130s for 25.0ml of remaining 1150.0ml @16:43:22.48 Intake.select :open @16:43:24.72 Intake @15.5psia, vacuum from 9.0 to 13.0psi @16:43:24.78 SS.reconfigure SSinhale.with maxSpeed: 17 @16:43:24.86 SS.seek 25ml @16:44:45.28 Vacuum too high -- waiting up to 40s @16:44:56.15 Intake @15.4psia, vacuum from 8.9 to 12.9psi @16:44:56.18 SS.reconfigure SSinhale.with maxSpeed: 15 @16:44:56.29 SS.seek 25ml @16:45:24.65 Stroke completed -- waiting for pressure to equalize ... @16:45:32.49 SS.reconfigure SSconfig @16:45:32.58 SS.hold @16:45:32.74 Intake.select :close @16:45:32.94 ShallowSampler::Clogged in WCR -- progress too slow after 375.0ml @16:45:32.96 SSV.dial :exhaust @16:45:33.91 Exhaust.select :open @16:45:34.05 SS.reconfigure SSexhale @16:45:34.13 SS.seek 0ml @16:45:43.81 Exhaust.select :close @16:45:43.97 Sampled 375.0ml @16:45:44.00 Drying puck before fixing for 4:00 @16:45:44.03 CBV.dial :waste2b @16:45:44.99 delay 1:00 @16:46:45.03 CSV.dial :air @16:46:45.96 CS.seek 10ml @16:47:13.29 Pumping wcr puck from tube 2 up to 55psi... @16:47:13.31 CBV.dial SSV @16:47:14.86 CBV.dial between CSV and waste2b @16:47:16.06 CSV.dial between air and CTV @16:47:16.46 CS.seek 7.355ml @16:47:24.45 CTV.dial CSV,avoiding: :air @16:47:24.49 CSV.dial CTV @16:47:25.48 CS.reconfigure CSpressurize @16:47:26.08 CS.seek 0ml @16:47:46.20 CSV.dial :air @16:47:46.81 CS.reconfigure CSfast @16:47:46.90 CS.seek 10ml @16:48:14.35 CSV.dial between air and CTV @16:48:14.79 CS.seek 2.16ml @16:48:36.52 CTV.dial CSV,avoiding: :air @16:48:36.55 CSV.dial CTV @16:48:36.96 CS.reconfigure CSpressurize @16:48:37.05 CS.seek 0ml @16:48:38.69 CBV.dial :waste2b @16:48:39.10 CS.reconfigure CSconfig @16:48:39.19 CS.seek :empty @16:48:44.92 delay 1:00 @16:49:44.95 CTV.dial :air,avoiding: :intake @16:49:46.59 Fix #1 of 2 (with rnal) for 2:00 @16:49:46.61 CSV.dial CSR @16:49:47.26 Pulling 2ml of CSR.rnal @16:49:47.28 CSR.select :rnal @16:49:47.46 CS.seek 2ml @16:49:53.82 delay 5 seconds @16:49:58.86 CSR.select :air @16:49:59.01 CS.reconfigure CSfast @16:49:59.09 CS.seek 10ml @16:50:21.20 Pumping wcr puck from tube 2 up to 55psi... @16:50:21.22 CBV.dial SSV @16:50:22.74 CBV.dial between CSV and waste2b @16:50:23.96 CSV.dial between air and CTV @16:50:24.74 CS.seek 7.276ml @16:50:32.89 CTV.dial CSV,avoiding: :air @16:50:32.93 CSV.dial CTV @16:50:34.65 CS.reconfigure CSpressurize @16:50:34.75 CS.seek 0ml @16:50:54.57 CSV.dial :air @16:50:55.15 CS.reconfigure CSfast @16:50:55.24 CS.seek 10ml @16:51:22.36 CSV.dial between air and CTV @16:51:22.79 CS.seek 2.19ml @16:51:44.36 CTV.dial CSV,avoiding: :air @16:51:44.40 CSV.dial CTV @16:51:44.82 CS.reconfigure CSpressurize @16:51:44.91 CS.seek 0ml @16:51:46.81 delay 2:00 @16:53:46.85 CBV.dial :waste2b @16:53:47.28 CS.reconfigure CSconfig @16:53:47.37 CS.seek :empty @16:53:52.98 delay 1:00 @16:54:53.01 CTV.dial :air,avoiding: :intake @16:54:54.65 Fix #2 of 2 (with rnal) for 2:00 @16:54:54.67 CSV.dial CSR @16:54:55.31 Pulling 2ml of CSR.rnal @16:54:55.34 CSR.select :rnal @16:54:55.57 CS.seek 2ml @16:55:01.92 delay 5 seconds @16:55:06.96 CSR.select :air @16:55:07.11 CS.reconfigure CSfast @16:55:07.67 CS.seek 10ml @16:55:29.51 Pumping wcr puck from tube 2 up to 55psi... @16:55:29.53 CBV.dial SSV @16:55:31.07 CBV.dial between CSV and waste2b @16:55:32.27 CSV.dial between air and CTV @16:55:33.04 CS.seek 7.267ml @16:55:41.45 CTV.dial CSV,avoiding: :air @16:55:41.49 CSV.dial CTV @16:55:43.17 CS.reconfigure CSpressurize @16:55:43.26 CS.seek 0ml @16:56:03.08 CSV.dial :air @16:56:03.78 CS.reconfigure CSfast @16:56:03.87 CS.seek 10ml @16:56:30.89 CSV.dial between air and CTV @16:56:31.31 CS.seek 2.237ml @16:56:52.77 CTV.dial CSV,avoiding: :air @16:56:52.81 CSV.dial CTV @16:56:53.20 CS.reconfigure CSpressurize @16:56:53.29 CS.seek 0ml @16:56:55.44 delay 2:00 @16:58:55.48 CBV.dial :waste2b @16:58:55.91 CS.reconfigure CSconfig @16:58:56.00 CS.seek :empty @16:59:01.49 delay 1:00 @17:00:01.53 CTV.dial :air,avoiding: :intake @17:00:03.25 Storing wcr puck in Collection Clamp to tube 1 @17:00:03.48 SC.reconfigure SCtight @17:00:03.57 SC.seek 1 @17:00:05.77 SC.reconfigure SCloose @17:00:06.04 Elbow.reconfigure TightElbow @17:00:06.14 Elbow.seek 1 @17:00:08.20 Forearm.reconfigure TightForearm @17:00:08.30 Forearm.seek 1 + 700 counts @17:00:09.43 SE.reconfigure SEpick @17:00:09.55 SE.seek beamBreak @17:00:12.06 SE.configure SEconfig @17:00:12.27 Elbow.reconfigure LimpElbow @17:00:12.37 Forearm.reconfigure LooseForearm @17:00:12.44 Forearm.seek 1 @17:00:12.78 Elbow.hold @17:00:12.89 Elbow.reconfigure TightElbow @17:00:13.03 SE.seek 8.1 Pucks below Top Plate @17:00:13.62 Forearm.force -20 @17:00:13.70 Forearm.seek 1 + 344 counts @17:00:14.08 Forearm.reconfigure TightForearm @17:00:14.16 Forearm.seek 1 + 700 counts @17:00:15.01 SE.seek 9.1 Pucks below Top Plate @17:00:15.80 Forearm.reconfigure LooseForearm @17:00:15.92 Forearm.seek :retract @17:00:16.92 Elbow.seek CC @17:00:18.63 Elbow.reconfigure LooseElbow @17:00:18.78 Forearm.seek CC @17:00:19.85 Hand.close @17:00:20.66 CC.seek :open @17:00:25.25 Forearm.seek :retract @17:00:26.34 Elbow.reconfigure TightElbow @17:00:26.43 Elbow.seek 1 @17:00:28.44 Elbow.reconfigure LimpElbow @17:00:28.54 Forearm.seek 1 @17:00:29.61 Elbow.hold @17:00:29.72 Elbow.reconfigure TightElbow @17:00:29.80 Hand.open @17:00:30.67 Forearm.force -20 @17:00:30.75 Forearm.seek 1 + 350 counts @17:00:31.12 Forearm.reconfigure TightForearm @17:00:31.20 Forearm.seek 1 + 700 counts @17:00:31.58 SE.seek :down @17:00:32.74 Forearm.reconfigure LooseForearm @17:00:32.85 Forearm.seek :retract @17:00:33.55 SE.coast @17:00:33.81 SC.coast @17:00:33.88 Starting finalPurge @17:00:33.90 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:00:34.02 Hand.open @17:00:34.41 Elbow.seek FlushPuck::Garage @17:00:36.64 Elbow.reconfigure LooseElbow @17:00:36.79 Forearm.seek :garage @17:00:37.87 Hand.close @17:00:38.79 Forearm.seek :retract @17:00:39.84 Elbow.reconfigure TightElbow @17:00:39.93 Elbow.seek CC @17:00:42.90 Elbow.reconfigure LooseElbow @17:00:43.04 Forearm.reconfigure TightForearm @17:00:43.12 Forearm.seek CC @17:00:44.46 CC.seek :closed @17:00:48.73 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.58 @17:00:48.87 Hand.open @17:00:49.74 Forearm.reconfigure LooseForearm @17:00:49.82 Forearm.seek :retract @17:00:50.92 Flushing the Collection Valve::Manifold @17:00:51.09 CSV.dial CSR @17:00:51.72 Pulling 5ml of CSR.flush @17:00:51.74 CSR.select :flush @17:00:51.90 CS.seek 5ml @17:01:05.93 delay 5 seconds @17:01:10.96 CSR.select :air @17:01:11.18 CS.reconfigure CSfast @17:01:11.27 CS.seek 7.5ml @17:01:18.67 CSV.dial :waste @17:01:20.28 CS.reconfigure CSconfig @17:01:20.37 CS.seek 0ml @17:01:40.68 Flushing lower manifold's waste1 port @17:01:40.85 CSV.dial :flush @17:01:41.41 CS.reconfigure CSfast @17:01:41.50 CS.seek 5ml @17:01:55.36 delay 5 seconds @17:02:00.39 CSV.dial :air @17:02:01.01 CS.seek 7ml @17:02:07.27 CSV.dial CTV @17:02:07.31
CTV.dial CSV @17:02:09.02 CBV.dial :waste1 @17:02:09.97 CS.seek 0ml @17:02:29.11 Flushing lower manifold's waste2b port @17:02:29.30 CSV.dial :flush @17:02:30.22 CS.seek 5ml @17:02:44.17 delay 5 seconds @17:02:49.20 CSV.dial :air @17:02:49.87 CS.seek 7ml @17:02:56.11 CSV.dial CTV @17:02:56.15
CTV.dial CSV @17:02:56.72 CBV.dial :waste2b @17:02:57.65 CS.seek 0ml @17:03:16.71 Flushing lower manifold's waste1 port @17:03:16.73 CSV.dial CSR @17:03:17.38 Pulling 4ml of CSR.mfbkill @17:03:17.39 CSR.select :mfbkill @17:03:17.57 CS.reconfigure CSconfig @17:03:17.65 CS.seek 4ml @17:03:29.08 delay 5 seconds @17:03:34.11 CSR.select :air @17:03:34.27 CS.reconfigure CSfast @17:03:34.35 CS.seek 10ml @17:03:50.83 CSV.dial CTV @17:03:50.87
CTV.dial CSV @17:03:51.46 CBV.dial :waste1 @17:03:52.40 CS.seek 0ml @17:04:19.18 Flushing lower manifold's waste2b port @17:04:19.20 CSV.dial CSR @17:04:19.97 Pulling 4ml of CSR.mfbkill @17:04:19.99 CSR.select :mfbkill @17:04:20.16 CS.reconfigure CSconfig @17:04:20.24 CS.seek 4ml @17:04:31.68 delay 5 seconds @17:04:36.71 CSR.select :air @17:04:36.86 CS.reconfigure CSfast @17:04:36.94 CS.seek 10ml @17:04:53.39 CSV.dial CTV @17:04:53.43
CTV.dial CSV @17:04:54.00 CBV.dial :waste2b @17:04:54.97 CS.seek 0ml @17:05:21.83 Killing upper manifold @17:05:21.85 CSV.dial CSR @17:05:22.49 Pulling 4ml of CSR.mfbkill @17:05:22.51 CSR.select :mfbkill @17:05:23.19 CS.reconfigure CSconfig @17:05:23.27 CS.seek 4ml @17:05:34.58 delay 5 seconds @17:05:39.61 CSR.select :air @17:05:39.76 CS.reconfigure CSfast @17:05:39.84 CS.seek 10ml @17:05:56.33 CSV.dial CBV @17:05:56.37
CBV.dial CSV @17:05:57.69 CTV.dial :air @17:05:59.31 CS.seek 0ml @17:06:26.43 delay 7 seconds @17:06:33.47 CS.seek 3ml @17:06:42.11 CSV.dial :waste @17:06:42.67 CS.seek 0ml @17:06:51.46 Flushing upper manifold @17:06:51.63 CSV.dial :flush @17:06:52.19 CS.seek 5ml @17:07:06.11 delay 5 seconds @17:07:11.14 CSV.dial :air @17:07:11.76 CS.seek 7ml @17:07:17.99 CSV.dial CBV @17:07:18.03
CBV.dial CSV @17:07:19.30 CTV.dial :air @17:07:19.34 CS.seek 0ml @17:07:38.21 delay 7 seconds @17:07:45.25 CS.seek 3ml @17:07:53.87 CSV.dial :waste @17:07:54.45 CS.seek 0ml @17:08:03.21 CSV.dial :flush @17:08:03.78 CS.seek 3ml @17:08:12.55 delay 5 seconds @17:08:17.58 CSV.dial :air @17:08:18.33 CS.seek 5ml @17:08:24.55 CSV.dial :air @17:08:24.58 CS.seek 0ml @17:08:38.46 delay 7 seconds @17:08:45.50 CS.seek 3ml @17:08:54.26 CSV.dial :waste @17:08:55.19 CS.seek 0ml @17:09:03.93 Flushing lower manifold's waste1 port @17:09:04.10 CSV.dial :flush @17:09:04.69 CS.seek 5ml @17:09:18.55 delay 5 seconds @17:09:23.58 CSV.dial :air @17:09:24.21 CS.seek 7ml @17:09:30.37 CSV.dial CTV @17:09:30.41
CTV.dial CSV @17:09:32.09 CBV.dial :waste1 @17:09:32.67 CS.seek 0ml @17:09:51.65 Flushing lower manifold's waste2b port @17:09:51.82 CSV.dial :flush @17:09:52.75 CS.seek 5ml @17:10:06.62 delay 5 seconds @17:10:11.65 CSV.dial :air @17:10:12.31 CS.seek 7ml @17:10:18.52 CSV.dial CTV @17:10:18.56
CTV.dial CSV @17:10:19.12 CBV.dial :waste2b @17:10:20.08 CS.seek 0ml @17:10:39.02 Garaging FlushPuck in Collection Clamp @17:10:39.14 Elbow.reconfigure TightElbow @17:10:39.21 Elbow.seek CC @17:10:39.49 Elbow.reconfigure LooseElbow @17:10:39.63 Forearm.seek CC @17:10:40.70 Hand.close @17:10:41.54 CC.seek :open @17:10:46.47 Forearm.seek :retract @17:10:47.56 Elbow.reconfigure TightElbow @17:10:47.65 Elbow.seek :garage @17:10:50.91 Elbow.reconfigure LooseElbow @17:10:51.06 Forearm.reconfigure TightForearm @17:10:51.14 Forearm.seek :garage @17:10:52.33 Hand.open @17:10:53.24 Forearm.reconfigure LooseForearm @17:10:53.32 Forearm.seek :retract @17:10:54.41 Completed [wcrphase] begun 16:15:18 @17:10:54.49 Asleep after completing 20invasivedeploywcrtest assays @17:10:54.51 Mission runtime was 56:29.648994 @17:10:54.54 GoodNews.email "{1 => 10, 2 => 0}\nCan@17:04:21, 21.9C, 29% humidity, 14.7psia, 11.952V, 0.638A, 0.638A avg, 7.63W",Subject: "Asleep after completing 20invasivedeploywcrtest assays" @17:11:03.35 Session Ended @17:11:03.42 Session Ended @13:31:31.90CEST07-May-20
ESP2 v3.30 I2C Gateway v3.06 for core bus @13:31:34.90 Copyright 2018 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @13:31:35.21 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @13:31:42.58 Polling Can every 10:00 @13:31:44.40 SPE.coast @13:31:44.46 SPE.configure SPEconfig @13:31:44.55 AS.configure ASconfig @13:31:44.65 SS.reconfigure SSconfig @13:31:44.70 SS.hold @13:31:44.75 SS.configure SSconfig @13:31:44.85 CC.reconfigure CCconfig @13:31:44.89 CC.hold @13:31:44.96 CC.configure CCconfig @13:31:45.06 CS.reconfigure CSconfig @13:31:45.10 CS.hold @13:31:45.18 CS.configure CSconfig @13:31:45.28 PC.reconfigure PCconfig @13:31:45.32 PC.hold @13:31:46.13 PC.configure PCconfig @13:31:46.33 PS.configure PSconfig @13:31:46.45 Forearm.reconfigure LooseForearm @13:31:46.49 Forearm.hold @13:31:46.55 Forearm.configure LooseForearm @13:31:46.64 Elbow.reconfigure LooseElbow @13:31:46.69 Elbow.hold @13:31:46.74 Elbow.configure LooseElbow @13:31:46.85 SC.reconfigure SCloose @13:31:46.90 SC.hold @13:31:46.99 SC.configure SCloose @13:31:47.20 SE.reconfigure SEconfig @13:31:47.28 SE.hold @13:31:47.40 SE.configure SEconfig @13:31:47.48 CH.coast @13:31:47.59 CH.configure CHconfig @13:31:47.66 PH.coast @13:31:47.78 PH.configure PHconfig @13:31:47.86 PCR.off @13:31:47.90 PCR.pidtemp= 50,0.1,0,300,25,25 @13:31:47.93 PCR.caltemp= 2.8814,0.0372,0 @13:31:47.96 All dwarf controllers running firmware version 3.75 @13:31:48.07 Cleanly exited at 13:30:12CEST07-May-20 @13:31:48.11 Loading /home/esp/esp2/mission/20invasivedeploymfbtest.rb mission @13:31:48.15 RuntimeError in MAIN -- Mission's startTube should be 3, not 2 @13:31:48.17 Waiting to Resume from Checkpoint "Loading /home/esp/esp2/mission/20invasivedeploymfbtest.rb mission" @13:31:48.19 BadNews.email "Mission's startTube should be 3, not 2",Subject: "RuntimeError in MAIN" @13:31:49.27 Session Begun @13:31:53.05 -> showlog 100 @13:32:00.78 -> exit @13:32:00.81 Session Ended @13:32:25.06 Session Begun Log::Locked in trapHandler -- Another process is already writing to /var/log/esp/real.log @13:32:30.29 -> showlog 100 @13:36:17.23CEST07-May-20
ESP2 v3.30 I2C Gateway v3.06 for core bus @13:36:20.10 Copyright 2018 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @13:36:20.43 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @13:36:27.73 Polling Can every 10:00 @13:36:29.87 SPE.coast @13:36:29.99 SPE.configure SPEconfig @13:36:30.18 AS.configure ASconfig @13:36:30.32 SS.reconfigure SSconfig @13:36:30.37 SS.hold @13:36:30.42 SS.configure SSconfig @13:36:30.51 CC.reconfigure CCconfig @13:36:30.56 CC.hold @13:36:30.62 CC.configure CCconfig @13:36:30.71 CS.reconfigure CSconfig @13:36:30.76 CS.hold @13:36:30.85 CS.configure CSconfig @13:36:30.94 PC.reconfigure PCconfig @13:36:30.99 PC.hold @13:36:31.79 PC.configure PCconfig @13:36:31.97 PS.configure PSconfig @13:36:32.11 Forearm.reconfigure LooseForearm @13:36:32.16 Forearm.hold @13:36:32.21 Forearm.configure LooseForearm @13:36:32.31 Elbow.reconfigure LooseElbow @13:36:32.35 Elbow.hold @13:36:32.42 Elbow.configure LooseElbow @13:36:32.54 SC.reconfigure SCloose @13:36:32.59 SC.hold @13:36:32.65 SC.configure SCloose @13:36:32.75 SE.reconfigure SEconfig @13:36:32.80 SE.hold @13:36:32.85 SE.configure SEconfig @13:36:32.91 CH.coast @13:36:32.96 CH.configure CHconfig @13:36:32.99 PH.coast @13:36:33.05 PH.configure PHconfig @13:36:33.10 PCR.off @13:36:33.14 PCR.pidtemp= 50,0.1,0,300,25,25 @13:36:33.18 PCR.caltemp= 2.8814,0.0372,0 @13:36:33.22 All dwarf controllers running firmware version 3.75 @13:36:33.38 Cleanly exited at 13:35:57CEST07-May-20 @13:36:33.45 Loading /home/esp/esp2/mission/20invasivedeploymfbtest.rb mission @13:36:33.61 Preparing to sleep until +0:00:10 @13:36:33.66 GoodNews.email "Starting 20invasivedeploymfbtest w/pucks in tube 2\n{}\nCan@13:36:27, 21.3C, 32% humidity, 14.7psia, 11.898V, 0.777A, 0.771A avg, 9.25W",Subject: "20invasivedeploymfbtest begun" @13:36:33.85 SS.coast @13:36:33.90
Asleep til +0:00:10 @13:36:34.01 Continuing at 13:36 @13:36:34.03 CC.coast @13:36:34.24 CS.coast @13:36:34.29 Session Begun @13:36:34.42 PC.coast @13:36:34.57 Forearm.coast @13:36:34.73 Elbow.coast @13:36:34.79 SC.coast @13:36:34.86 SE.coast @13:36:35.38 Gate.power :core,:OFF @13:36:35.42 Gate.power :raw,:OFF @13:36:35.47 Shutdown @13:36:41.07 GoodNews.email "Then, in tube 2: mbaPhase\n/home/esp/esp2/mission/20invasivedeploymfbtest.rb:119\n{}\nCan@13:36:34, 21.3C, 32% humidity, 14.7psia, 11.898V, 0.777A, 0.777A avg, 9.25W",Subject: "Asleep until 13:36" @13:36:43.47
Powering core bus @13:36:48.73 Gate.power :raw,:ON @13:36:50.58 SPE.coast @13:36:50.86 SPE.configure SPEconfig @13:36:51.22 AS.configure ASconfig @13:36:51.66 SS.configure SSconfig @13:36:52.06 CC.configure CCconfig @13:36:52.51 CS.configure CSconfig @13:36:52.90 PC.configure PCconfig @13:36:53.28 PS.configure PSconfig @13:36:53.73 Forearm.configure LooseForearm @13:36:54.09 Elbow.configure LooseElbow @13:36:54.52 SC.configure SCloose @13:36:54.90 SE.configure SEconfig @13:36:55.09 CH.coast @13:36:55.33 CH.configure CHconfig @13:36:55.48 PH.coast @13:36:55.70 PH.configure PHconfig @13:36:55.90 PCR.off @13:36:56.03 PCR.pidtemp= 50,0.1,0,300,25,25 @13:36:56.13 PCR.caltemp= 2.8814,0.0372,0 @13:36:56.29 All dwarf controllers running firmware version 3.75 @13:36:56.56 Valve.reset @13:36:57.01 PCR.off @13:36:57.16 PCR.pidtemp= 50,0.1,0,300,25,25 @13:36:57.26 PCR.caltemp= 2.8814,0.0372,0 @13:36:57.81 Intake.select :close @13:36:57.90 Exhaust.select :close @13:36:58.07 CTV.dial :air @13:36:58.41 PSV.dial :air @13:36:58.55 CSV.dial :waste1 @13:36:59.13 SSV.dial :spit,avoiding: :exhaust @13:36:59.27 PS.reconfigure PSconfig @13:36:59.37
Forearm.reconfigure LooseForearm @13:36:59.79 PS.home.seek 0ml @13:37:00.08
Forearm.home.seek :retract @13:37:01.83 CS.reconfigure CSfast @13:37:01.95 SS.reconfigure SSconfig @13:37:02.22 CS.home.seek 0ml @13:37:02.53 SS.home.seek 0ml @13:37:02.78 Hand.close @13:37:02.95
Elbow.jog 1500 @13:37:03.75 Elbow.reconfigure LooseElbow @13:37:03.96 Elbow.home.seek :clear @13:37:08.96 SE.reconfigure SEconfig @13:37:09.19 SE.home.seek :down @13:37:09.99 SC.reconfigure SCsloppy @13:37:10.20 SC.home.seek :initial @13:37:10.43 PS.coast @13:37:14.33
Forearm.seek :retract @13:37:14.61 Elbow.seek :hammer @13:37:15.97 Forearm.reconfigure TightForearm @13:37:16.18 Forearm.seek 5 + 350 counts @13:37:17.51 Forearm.seek :retract @13:37:19.27 PC.reconfigure PCconfig @13:37:19.37
CC.reconfigure CCconfig @13:37:19.74 CC.home.seek :open @13:37:19.82 PC.home.seek :open @13:37:24.18
Phase [mbaPhase] @13:37:24.24 GoodNews.email "At 13:37:23 began in tube 2: mbaPhase\n/home/esp/esp2/mission/20invasivedeploymfbtest.rb:119\n{}\nCan@13:37:24, 21.3C, 32% humidity, 14.7psia, 11.898V, 0.777A, 0.777A avg, 9.25W",Subject: "Started 20invasivedeploymfbtest phase mbaPhase" @13:37:24.43CEST07-May-20
===> STARTED Superphase (phase #1) <=== Everybody has a plan untill they get punched in the face - Mike Tyson @13:37:24.82 Starting mfb @13:37:24.85 pcrBAC sampling at most 1500ml, wcr at most 1500ml @13:37:25.19 Loading sh1 puck from tube 2 into Collection Clamp @13:37:25.54 Readying the MFB @13:37:25.68
Hand.open @13:37:25.78 Homing MFB Syringe @13:37:25.91 AFV.select :holdingCoil @13:37:26.57 ATV.dial :air2 @13:37:27.90 AS.reconfigure ASconfig @13:37:28.20 AS.home.seek :home @13:37:28.85
SC.reconfigure SCloose @13:37:29.81 Elbow.reconfigure TightElbow @13:37:30.04 Elbow.seek 2 @13:37:31.25 Forearm.seek 2 + 700 counts @13:37:32.95 SE.reconfigure SEpick @13:37:33.26 SE.seek beamBreak @13:37:33.55 BACfans.email "Started mfb in tube 2 at 13:37:24CEST07-May-20\n{}\nCan@13:37:24, 21.3C, 32% humidity, 14.7psia, 11.898V, 0.777A, 0.777A avg, 9.25W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" @13:37:34.40 AFV.select :carrierSolution @13:37:34.87 delay 2 seconds @13:37:36.92
SE.configure SEconfig @13:37:38.13 AS.seek 1ml @13:37:38.78
Elbow.seek 2 @13:37:40.01 Forearm.seek 2 + 300 counts @13:37:40.53 Forearm.reconfigure LooseForearm @13:37:40.84 Hand.close @13:37:42.17 SE.seek :down @13:37:43.51
Forearm.seek :retract @13:37:45.02 Elbow.seek CC @13:37:45.57 SE.coast @13:37:47.41
Elbow.reconfigure LooseElbow @13:37:48.26 Forearm.reconfigure TightForearm @13:37:48.64 Forearm.seek CC @13:37:51.93 CC.seek :closed @13:38:37.37 delay 1 second @13:38:37.48
Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.55 @13:38:37.56 Hand.open @13:38:37.86 I2C::UnexpectedReply in core -- I2C::Servo::Status3Reply[6E:27->20] @13:38:37.95 I2C::UnexpectedReply in core -- I2C::Servo::Status3Reply[6E:27->20] @13:38:38.62 Forearm.reconfigure LooseForearm @13:38:38.82 Forearm.seek :retract @13:38:39.03 AFV.select :holdingCoil @13:38:39.23 delay 1 second @13:38:40.02
SC.coast @13:38:40.24 collectSample 1500ml w/bubble point of 55psi @13:38:40.32 Priming 7ml @13:38:40.41 Intake.select :close @13:38:40.47 Exhaust.select :close @13:38:40.55 SS.hold @13:38:41.03 ATV.dial :waste @13:38:41.55
Calibrating pressure and temperature sensors @13:38:41.61 CTV.dial :air @13:38:41.70 SSV.dial :spit,avoiding: :exhaust @13:38:41.80 SS.reconfigure SSfast @13:38:42.03 SS.seek 5ml @13:38:42.33 AS.reconfigure ASnorm @13:38:42.52 AS.home.seek :empty @13:38:45.22 SSV.dial CBV @13:38:45.34
CBV.dial SSV @13:38:47.08 delay 3.8 seconds @13:38:51.28 SSV.dial :spit @13:38:52.97 SS.seek 0ml @13:38:55.70 SSV.dial CBV @13:38:57.34 CTV.dial :intake @13:38:58.42 SSV.dial CBV @13:38:58.51
CBV.dial SSV @13:38:58.55 Allowing 40s for 7.0ml of remaining 7.0ml @13:38:58.66 Intake.select :open @13:39:01.13 Intake @15.4psia, vacuum from 8.9 to 12.9psi @13:39:01.19 SS.reconfigure SSinhale.with maxSpeed: 150 @13:39:01.44 SS.seek 7ml @13:39:07.48 Stroke completed -- waiting for pressure to equalize ... @13:39:23.20 Intake.select :close @13:39:23.38 SSV.dial :exhaust @13:39:24.26 Exhaust.select :open @13:39:24.40 SS.reconfigure SSexhale @13:39:24.46 SS.seek 0ml @13:39:28.37 Exhaust.select :close @13:39:28.50 Sampling 1500.0ml @13:39:28.52 Intake.select :close @13:39:28.54 Exhaust.select :close @13:39:28.57 SS.reconfigure SSconfig @13:39:28.65 SS.hold @13:39:28.89 CTV.dial :intake @13:39:28.91 SSV.dial CBV @13:39:28.95
CBV.dial SSV @13:39:29.84 Allowing 130s for 25.0ml of remaining 1500.0ml @13:39:29.85 Intake.select :open @13:39:32.06 Intake @15.4psia, vacuum from 8.9 to 12.9psi @13:39:32.09 SS.reconfigure SSinhale.with maxSpeed: 150 @13:39:32.17 SS.seek 25ml @13:39:35.21 ATV.dial :waste @13:39:35.22 ARV.dial between carrier and air1 @13:39:35.33 ASV.dial :waste1 @13:39:36.61
Vacuum too high -- waiting up to 40s @13:39:43.76 Intake @16.0psia, vacuum from 9.5 to 13.5psi @13:39:43.78 SS.reconfigure SSinhale.with maxSpeed: 135 @13:39:43.86 SS.seek 25ml @13:39:47.15 Vacuum too high -- waiting up to 40s @13:39:54.02 Intake @16.9psia, vacuum from 10.4 to 14.4psi @13:39:54.05 SS.reconfigure SSinhale.with maxSpeed: 122 @13:39:54.13 SS.seek 25ml @13:39:57.15 Vacuum too high -- waiting up to 40s @13:40:02.73 Intake @17.0psia, vacuum from 10.5 to 14.5psi @13:40:02.76 SS.reconfigure SSinhale.with maxSpeed: 110 @13:40:02.84 SS.seek 25ml @13:40:05.56 Vacuum too high -- waiting up to 40s @13:40:10.54 Intake @17.1psia, vacuum from 10.6 to 14.6psi @13:40:10.57 SS.reconfigure SSinhale.with maxSpeed: 99 @13:40:10.66 SS.seek 25ml @13:40:13.31 Vacuum too high -- waiting up to 40s @13:40:17.38 Intake @17.2psia, vacuum from 10.7 to 14.7psi @13:40:17.41 SS.reconfigure SSinhale.with maxSpeed: 89 @13:40:17.49 SS.seek 25ml @13:40:19.77 Vacuum too high -- waiting up to 40s @13:40:24.59 Intake @17.4psia, vacuum from 10.9 to 14.9psi @13:40:24.62 SS.reconfigure SSinhale.with maxSpeed: 80 @13:40:24.70 SS.seek 25ml @13:40:27.50 Vacuum too high -- waiting up to 40s @13:40:31.61 Intake @17.4psia, vacuum from 10.9 to 14.9psi @13:40:31.64 SS.reconfigure SSinhale.with maxSpeed: 72 @13:40:31.73 SS.seek 25ml @13:40:34.75 Vacuum too high -- waiting up to 40s @13:40:38.68 Intake @17.2psia, vacuum from 10.7 to 14.7psi @13:40:38.71 SS.reconfigure SSinhale.with maxSpeed: 65 @13:40:38.79 SS.seek 25ml @13:40:40.89 Vacuum too high -- waiting up to 40s @13:40:43.72 Intake @17.1psia, vacuum from 10.6 to 14.6psi @13:40:43.75 SS.reconfigure SSinhale.with maxSpeed: 59 @13:40:43.83 SS.seek 25ml @13:40:45.09 Stroke completed -- waiting for pressure to equalize ... @13:40:48.93 Intake.select :close @13:40:49.11 SSV.dial :exhaust @13:40:49.99 Exhaust.select :open @13:40:50.13 SS.reconfigure SSexhale @13:40:50.19 SS.seek 0ml @13:40:59.83 Exhaust.select :close @13:40:59.96 SSV.dial CBV @13:41:00.00
CBV.dial SSV @13:41:00.90 Allowing 130s for 25.0ml of remaining 1475.0ml @13:41:00.92 Intake.select :open @13:41:03.12 Intake @15.8psia, vacuum from 9.3 to 13.3psi @13:41:03.14 SS.reconfigure SSinhale.with maxSpeed: 59 @13:41:03.22 SS.seek 25ml @13:41:06.29 Vacuum too high -- waiting up to 40s @13:41:09.12 Intake @16.6psia, vacuum from 10.1 to 14.1psi @13:41:09.15 SS.reconfigure SSinhale.with maxSpeed: 53 @13:41:09.23 SS.seek 25ml @13:41:14.22 Vacuum too high -- waiting up to 40s @13:41:18.57 Intake @16.4psia, vacuum from 9.9 to 13.9psi @13:41:18.60 SS.reconfigure SSinhale.with maxSpeed: 48 @13:41:18.68 SS.seek 25ml @13:41:23.64 Vacuum too high -- waiting up to 40s @13:41:27.73 Intake @16.7psia, vacuum from 10.2 to 14.2psi @13:41:27.75 SS.reconfigure SSinhale.with maxSpeed: 43 @13:41:27.84 SS.seek 25ml @13:41:31.17 Vacuum too high -- waiting up to 40s @13:41:33.74 Intake @16.9psia, vacuum from 10.4 to 14.4psi @13:41:33.77 SS.reconfigure SSinhale.with maxSpeed: 39 @13:41:33.85 SS.seek 25ml @13:41:39.96 Vacuum too high -- waiting up to 40s @13:41:43.54 Intake @17.0psia, vacuum from 10.5 to 14.5psi @13:41:43.57 SS.reconfigure SSinhale.with maxSpeed: 35 @13:41:43.65 SS.seek 25ml @13:41:49.46 Vacuum too high -- waiting up to 40s @13:41:52.29 Intake @16.9psia, vacuum from 10.4 to 14.4psi @13:41:52.32 SS.reconfigure SSinhale.with maxSpeed: 32 @13:41:52.40 SS.seek 25ml @13:42:00.49 Vacuum too high -- waiting up to 40s @13:42:03.58 Intake @16.9psia, vacuum from 10.4 to 14.4psi @13:42:03.60 SS.reconfigure SSinhale.with maxSpeed: 29 @13:42:03.68 SS.seek 25ml @13:42:18.48 Stroke completed -- waiting for pressure to equalize ... @13:42:24.81 Intake.select :close @13:42:24.97 SSV.dial :exhaust @13:42:25.87 Exhaust.select :open @13:42:26.01 SS.reconfigure SSexhale @13:42:26.08 SS.seek 0ml @13:42:35.66 Exhaust.select :close @13:42:35.79 SSV.dial CBV @13:42:35.83
CBV.dial SSV @13:42:36.72 Allowing 130s for 25.0ml of remaining 1450.0ml @13:42:36.73 Intake.select :open @13:42:38.94 Intake @15.8psia, vacuum from 9.3 to 13.3psi @13:42:38.96 SS.reconfigure SSinhale.with maxSpeed: 29 @13:42:39.05 SS.seek 25ml @13:42:59.74 Vacuum too high -- waiting up to 40s @13:43:03.83 Intake @16.7psia, vacuum from 10.2 to 14.2psi @13:43:03.85 SS.reconfigure SSinhale.with maxSpeed: 26 @13:43:03.94 SS.seek 25ml @13:43:30.78 Vacuum too high -- waiting up to 40s @13:43:34.92 Intake @16.7psia, vacuum from 10.2 to 14.2psi @13:43:34.95 SS.reconfigure SSinhale.with maxSpeed: 23 @13:43:35.03 SS.seek 25ml @13:43:55.84 Stroke completed -- waiting for pressure to equalize ... @13:44:01.55 Intake.select :close @13:44:01.79 SSV.dial :exhaust @13:44:02.75 Exhaust.select :open @13:44:02.92 SS.reconfigure SSexhale @13:44:02.99 SS.seek 0ml @13:44:12.82 Exhaust.select :close @13:44:12.94 SSV.dial CBV @13:44:12.96
CBV.dial SSV @13:44:13.85 Allowing 130s for 25.0ml of remaining 1425.0ml @13:44:13.87 Intake.select :open @13:44:16.05 Intake @15.8psia, vacuum from 9.3 to 13.3psi @13:44:16.07 SS.reconfigure SSinhale.with maxSpeed: 23 @13:44:16.14 SS.seek 25ml @13:45:29.02 Vacuum too high -- waiting up to 40s @13:45:33.45 Intake @16.5psia, vacuum from 10.0 to 14.0psi @13:45:33.83 SS.reconfigure SSinhale.with maxSpeed: 21 @13:45:33.97 SS.seek 25ml @13:45:38.25 Stroke completed -- waiting for pressure to equalize ... @13:45:41.97 Intake.select :close @13:45:42.13 SSV.dial :exhaust @13:45:43.00 Exhaust.select :open @13:45:43.13 SS.reconfigure SSexhale @13:45:43.20 SS.seek 0ml @13:45:52.77 Exhaust.select :close @13:45:52.94 SSV.dial CBV @13:45:52.96
CBV.dial SSV @13:45:53.85 Allowing 130s for 25.0ml of remaining 1400.0ml @13:45:53.87 Intake.select :open @13:45:56.05 Intake @15.7psia, vacuum from 9.2 to 13.2psi @13:45:56.07 SS.reconfigure SSinhale.with maxSpeed: 21 @13:45:56.14 SS.seek 25ml @13:47:19.54 Stroke completed -- waiting for pressure to equalize ... @13:47:26.35 Intake.select :close @13:47:26.51 SSV.dial :exhaust @13:47:27.39 Exhaust.select :open @13:47:27.52 SS.reconfigure SSexhale @13:47:27.59 SS.seek 0ml @13:47:37.24 Exhaust.select :close @13:47:37.36 SSV.dial CBV @13:47:37.38
CBV.dial SSV @13:47:38.28 Allowing 130s for 25.0ml of remaining 1375.0ml @13:47:38.30 Intake.select :open @13:47:40.48 Intake @15.8psia, vacuum from 9.3 to 13.3psi @13:47:40.50 SS.reconfigure SSinhale.with maxSpeed: 21 @13:47:40.57 SS.seek 25ml @13:49:04.17 Stroke completed -- waiting for pressure to equalize ... @13:49:12.16 Intake.select :close @13:49:12.31 SSV.dial :exhaust @13:49:13.18 Exhaust.select :open @13:49:13.31 SS.reconfigure SSexhale @13:49:13.38 SS.seek 0ml @13:49:23.32 Exhaust.select :close @13:49:23.44 SSV.dial CBV @13:49:23.46
CBV.dial SSV @13:49:24.35 Allowing 130s for 25.0ml of remaining 1350.0ml @13:49:24.37 Intake.select :open @13:49:26.55 Intake @15.7psia, vacuum from 9.2 to 13.2psi @13:49:26.57 SS.reconfigure SSinhale.with maxSpeed: 21 @13:49:26.64 SS.seek 25ml @13:50:50.76 Stroke completed -- waiting for pressure to equalize ... @13:51:00.39 Intake.select :close @13:51:00.55 SSV.dial :exhaust @13:51:01.42 Exhaust.select :open @13:51:01.55 SS.reconfigure SSexhale @13:51:01.62 SS.seek 0ml @13:51:10.88 Exhaust.select :close @13:51:11.00 SSV.dial CBV @13:51:11.02
CBV.dial SSV @13:51:11.93 Allowing 130s for 25.0ml of remaining 1325.0ml @13:51:11.94 Intake.select :open @13:51:14.12 Intake @15.8psia, vacuum from 9.3 to 13.3psi @13:51:14.14 SS.reconfigure SSinhale.with maxSpeed: 21 @13:51:14.21 SS.seek 25ml @13:52:37.42 Vacuum too high -- waiting up to 40s @13:52:46.43 Intake @16.1psia, vacuum from 9.6 to 13.6psi @13:52:46.44 SS.reconfigure SSinhale.with maxSpeed: 19 @13:52:46.52 SS.seek 25ml @13:52:48.34 Stroke completed -- waiting for pressure to equalize ... @13:52:53.09 Intake.select :close @13:52:53.25 SSV.dial :exhaust @13:52:54.12 Exhaust.select :open @13:52:54.25 SS.reconfigure SSexhale @13:52:54.32 SS.seek 0ml @13:53:03.95 Exhaust.select :close @13:53:04.08 SSV.dial CBV @13:53:04.10
CBV.dial SSV @13:53:04.99 Allowing 130s for 25.0ml of remaining 1300.0ml @13:53:05.01 Intake.select :open @13:53:07.19 Intake @15.8psia, vacuum from 9.3 to 13.3psi @13:53:07.21 SS.reconfigure SSinhale.with maxSpeed: 19 @13:53:07.29 SS.seek 25ml @13:54:40.13 Stroke completed -- waiting for pressure to equalize ... @13:54:53.65 Intake.select :close @13:54:53.81 SSV.dial :exhaust @13:54:54.70 Exhaust.select :open @13:54:54.82 SS.reconfigure SSexhale @13:54:54.89 SS.seek 0ml @13:55:04.60 Exhaust.select :close @13:55:04.72 SSV.dial CBV @13:55:04.74
CBV.dial SSV @13:55:05.63 Allowing 130s for 25.0ml of remaining 1275.0ml @13:55:05.65 Intake.select :open @13:55:07.83 Intake @15.9psia, vacuum from 9.4 to 13.4psi @13:55:07.85 SS.reconfigure SSinhale.with maxSpeed: 19 @13:55:07.92 SS.seek 25ml @13:56:20.36 Vacuum too high -- waiting up to 40s @13:56:29.14 Intake @16.0psia, vacuum from 9.5 to 13.5psi @13:56:29.16 SS.reconfigure SSinhale.with maxSpeed: 17 @13:56:29.23 SS.seek 25ml @13:56:52.77 Stroke completed -- waiting for pressure to equalize ... @13:57:02.57 Intake.select :close @13:57:02.73 SSV.dial :exhaust @13:57:03.63 Exhaust.select :open @13:57:03.75 SS.reconfigure SSexhale @13:57:03.82 SS.seek 0ml @13:57:13.55 Exhaust.select :close @13:57:13.67 SSV.dial CBV @13:57:13.69
CBV.dial SSV @13:57:14.59 Allowing 130s for 25.0ml of remaining 1250.0ml @13:57:14.61 Intake.select :open @13:57:16.81 Intake @15.7psia, vacuum from 9.2 to 13.2psi @13:57:16.83 SS.reconfigure SSinhale.with maxSpeed: 17 @13:57:16.90 SS.seek 25ml @13:59:01.27 Stroke completed -- waiting for pressure to equalize ... @13:59:17.04 Intake.select :close @13:59:17.19 SSV.dial :exhaust @13:59:18.08 Exhaust.select :open @13:59:18.20 SS.reconfigure SSexhale @13:59:18.27 SS.seek 0ml @13:59:27.98 Exhaust.select :close @13:59:28.11 SSV.dial CBV @13:59:28.13
CBV.dial SSV @13:59:29.02 Allowing 130s for 25.0ml of remaining 1225.0ml @13:59:29.04 Intake.select :open @13:59:31.22 Intake @15.8psia, vacuum from 9.3 to 13.3psi @13:59:31.24 SS.reconfigure SSinhale.with maxSpeed: 17 @13:59:31.31 SS.seek 25ml @14:01:11.26 Vacuum too high -- waiting up to 40s @14:01:25.13 Intake @16.0psia, vacuum from 9.5 to 13.5psi @14:01:25.14 SS.reconfigure SSinhale.with maxSpeed: 15 @14:01:25.22 SS.seek 25ml @14:01:31.04 Stroke completed -- waiting for pressure to equalize ... @14:01:39.05 SS.reconfigure SSconfig @14:01:39.12 SS.hold @14:01:39.25 Intake.select :close @14:01:39.40 ShallowSampler::Clogged in MAIN -- progress too slow after 300.0ml @14:01:39.42 SSV.dial :exhaust @14:01:40.30 Exhaust.select :open @14:01:40.43 SS.reconfigure SSexhale @14:01:40.50 SS.seek 0ml @14:01:49.83 Exhaust.select :close @14:01:49.95 Sampled 300.0ml @14:01:49.97 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @14:01:49.98 Intake.select :close @14:01:50.06 CSV.dial :waste @14:01:50.14 CS.seek 0ml @14:01:50.22 SSV.dial CBV,avoiding: :exhaust @14:01:50.25
CBV.dial SSV @14:01:50.44 CSV.dial :air @14:01:51.34 CS.seek 10ml @14:01:52.44
CTV.dial :air @14:01:53.34 delay 15 seconds @14:02:08.36 SSV.dial :spit,avoiding: :exhaust @14:02:09.88 CTV.dial CSV,avoiding: :intake @14:02:09.93
SS.seek 0ml @14:02:10.04 SSV.dial CBV,avoiding: :exhaust @14:02:18.85 CBV.dial between SSV and waste2 @14:02:19.30 CTV.dial CSV,avoiding: :air @14:02:19.33
CSV.dial CTV @14:02:19.86 CS.reconfigure CSpressurize @14:02:19.93 CS.seek 0ml @14:02:46.45 CSV.dial :air @14:02:47.00 CS.reconfigure CSfast @14:02:47.06 CS.seek 10ml @14:03:13.88 CSV.dial between air and CTV @14:03:14.27 CS.seek 2.461ml @14:03:34.80 CTV.dial CSV,avoiding: :air @14:03:34.83
CSV.dial CTV @14:03:35.17 CS.reconfigure CSpressurize @14:03:35.23 CS.seek 0ml @14:03:38.70 BubblePoint: Intake @71.4psia, Outlet @15.9psia @14:03:38.71 CBV.dial SSV @14:03:39.11 SS.reconfigure SSconfig @14:03:39.60 SS.seek 10ml @14:03:45.47 delay 15 seconds @14:04:00.60 Evacuated: Intake @37.3psia, Outlet @14.0psia @14:04:00.61 SSV.dial :spit,avoiding: :exhaust @14:04:02.16 SS.reconfigure SSexhale @14:04:02.22 SS.seek 0ml @14:04:07.54 SS.reconfigure SSfast @14:04:07.61 SS.seek 5ml @14:04:10.45 SSV.dial CBV,avoiding: :exhaust @14:04:10.47
CBV.dial SSV @14:04:12.02 CTV.dial :air,avoiding: :intake @14:04:13.61 CSV.dial CTV @14:04:13.65
SS.seek 10ml @14:04:14.63 CSV.dial :air @14:04:15.19 CS.reconfigure CSfast @14:04:15.25 CS.seek 0ml @14:04:16.63
SSV.dial :spit,avoiding: :exhaust @14:04:18.17 SS.reconfigure SSexhale @14:04:18.23 SS.seek 0ml @14:04:19.88 CS.coast @14:04:23.29
SS.reconfigure SSfast @14:04:23.37 SS.seek 5ml @14:04:26.23 SSV.dial CBV,avoiding: :exhaust @14:04:26.25
CBV.dial SSV @14:04:28.79 SSV.dial :spit,avoiding: :exhaust @14:04:30.34 SS.reconfigure SSconfig @14:04:30.40 SS.seek 0ml @14:04:33.47 SS.coast @14:04:33.52 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @14:04:33.53 Intake.select :close @14:04:33.63 CSV.dial :waste @14:04:33.75 SSV.dial CBV,avoiding: :exhaust @14:04:33.77
CBV.dial SSV @14:04:34.55 CS.seek 0ml @14:04:34.63 CSV.dial :air @14:04:35.34
CTV.dial :air @14:04:35.35 delay 15 seconds @14:04:35.50 CS.seek 10ml @14:04:50.38
SSV.dial :spit,avoiding: :exhaust @14:04:51.91 CTV.dial CSV,avoiding: :intake @14:04:51.96
SS.reconfigure SSexhale @14:04:52.06 SS.seek 0ml @14:04:52.13 SSV.dial CBV,avoiding: :exhaust @14:05:02.69 CBV.dial between SSV and waste2 @14:05:03.13 CSV.dial between air and CTV @14:05:03.50 CS.seek 8.141ml @14:05:09.30 CTV.dial CSV,avoiding: :air @14:05:09.32
CSV.dial CTV @14:05:09.69 CS.reconfigure CSpressurize @14:05:09.76 CS.seek 0ml @14:05:31.42 CSV.dial :air @14:05:31.96 CS.reconfigure CSfast @14:05:32.03 CS.seek 10ml @14:05:58.82 CSV.dial between air and CTV @14:05:59.21 CS.seek 3.062ml @14:06:18.11 CTV.dial CSV,avoiding: :air @14:06:18.14
CSV.dial CTV @14:06:18.49 CS.reconfigure CSpressurize @14:06:18.55 CS.seek 0ml @14:06:26.80 BubblePoint: Intake @70.7psia, Outlet @15.4psia @14:06:26.81 CBV.dial SSV @14:06:27.21 SS.reconfigure SSconfig @14:06:27.27 SS.seek 10ml @14:06:32.65 delay 15 seconds @14:06:47.78 Evacuated: Intake @39.3psia, Outlet @17.7psia @14:06:47.79 SSV.dial :spit,avoiding: :exhaust @14:06:49.34 SS.reconfigure SSexhale @14:06:49.40 SS.seek 0ml @14:06:54.70 SS.reconfigure SSfast @14:06:54.77 SS.seek 5ml @14:06:57.44 SSV.dial CBV,avoiding: :exhaust @14:06:57.46
CBV.dial SSV @14:06:59.01 CTV.dial :air,avoiding: :intake @14:07:00.61 CSV.dial CTV @14:07:00.65
SS.seek 10ml @14:07:01.64 CSV.dial :air @14:07:02.19 CS.reconfigure CSfast @14:07:02.25 CS.seek 0ml @14:07:03.44
SSV.dial :spit,avoiding: :exhaust @14:07:04.14 CS.coast @14:07:04.98
SS.reconfigure SSexhale @14:07:05.04 SS.seek 0ml @14:07:10.40 SS.reconfigure SSfast @14:07:10.47 SS.seek 5ml @14:07:13.12 SSV.dial CBV,avoiding: :exhaust @14:07:13.14
CBV.dial SSV @14:07:15.68 SSV.dial :spit,avoiding: :exhaust @14:07:17.22 SS.reconfigure SSconfig @14:07:17.28 SS.seek 0ml @14:07:20.58 SS.coast @14:07:20.64 Lysing: Adding 1.5ml lysis @14:07:20.65 CBV.dial between CSV and waste1,avoiding: :DBV @14:07:22.44 CTV.dial CSV @14:07:24.01 CSV.dial CSR @14:07:24.95 Pulling 1ml of CSR.lysis @14:07:24.96 CSR.select :lysis @14:07:25.09 CS.reconfigure CSconfig @14:07:25.15 CS.seek 1ml @14:07:28.85 delay 5 seconds @14:07:33.87 CSR.select :air @14:07:34.05 CS.seek 5.999ml @14:07:47.89 delay 2 seconds @14:07:49.92 CSV.dial :waste1 @14:07:51.44 CS.reconfigure CSfast @14:07:51.50 CS.seek :empty @14:08:07.89 CSV.dial CSR @14:08:09.49 Pulling 1.5ml of CSR.lysis @14:08:09.50 CSR.select :lysis @14:08:09.63 CS.reconfigure CSconfig @14:08:09.70 CS.seek 1.5ml @14:08:14.57 delay 5 seconds @14:08:19.59 CSR.select :air @14:08:19.78 CS.seek 4.5ml @14:08:28.48 delay 2 seconds @14:08:30.51 CSV.dial CTV @14:08:31.05 CS.seek 1.275ml @14:08:40.30 Heating sh1 puck from tube 2 in Collection Clamp to 85C for 5:00 @14:08:40.32 CH.seek 85C,8:00 @14:12:11.69 CH.hold 5:00,85C @14:17:11.86 Collection Puck Heater at 85C after lysing @14:17:11.88 CS.seek 0ml @14:17:16.31 CTV.dial between spare and air,avoiding: :intake @14:17:17.56 CSV.dial :air @14:17:18.09 CS.seek 5ml @14:17:32.27 CSV.dial CTV @14:17:32.81 delay 2 seconds @14:17:34.83 CSV.dial :air @14:17:35.36 delay 2 seconds @14:17:37.39 CSV.dial CTV @14:17:37.93 delay 2 seconds @14:17:39.95 CSV.dial :air @14:17:40.59 delay 2 seconds @14:17:42.62 CS.seek 0ml @14:17:56.33 Recovering Lysate into Collection syringe @14:17:56.34 CSV.dial CBV @14:17:57.59 CBV.dial CSV @14:17:58.04 CS.seek 5ml @14:18:11.80 delay 5 seconds @14:18:16.83 CTV.dial :air @14:18:17.43 CS.seek 7.001ml @14:18:23.67 delay 5 seconds @14:18:28.70 CSV.dial :air2 @14:18:29.21 CS.seek 10ml @14:18:38.02 CSV.dial between air and CTV,avoiding: CBV @14:18:39.40 Lysing Complete @14:18:39.48 Storing sh1 puck in Collection Clamp to tube 1 @14:18:40.11 SC.reconfigure SCtight @14:18:40.17 SC.seek 1 @14:18:42.31 SC.reconfigure SCloose @14:18:42.53 Elbow.reconfigure TightElbow @14:18:42.59 Elbow.seek 1 @14:18:44.60 Forearm.reconfigure TightForearm @14:18:44.66 Forearm.seek 1 + 700 counts @14:18:45.79 SE.reconfigure SEpick @14:18:45.87 SE.seek beamBreak @14:18:48.19 SE.configure SEconfig @14:18:48.36 Elbow.reconfigure LimpElbow @14:18:48.42 Forearm.reconfigure LooseForearm @14:18:48.49 Forearm.seek 1 @14:18:48.78 Elbow.hold @14:18:48.86 Elbow.reconfigure TightElbow @14:18:48.97 SE.seek 9.09 Pucks below Top Plate @14:18:49.47 Forearm.force -20 @14:18:49.53 Forearm.seek 1 + 348 counts @14:18:49.85 Forearm.reconfigure TightForearm @14:18:49.91 Forearm.seek 1 + 700 counts @14:18:50.21 SE.seek 10.09 Pucks below Top Plate @14:18:50.89 Forearm.reconfigure LooseForearm @14:18:50.95 Forearm.seek :retract @14:18:51.88 Elbow.seek CC @14:18:53.59 Elbow.reconfigure LooseElbow @14:18:53.70 Forearm.seek CC @14:18:54.77 Hand.close @14:18:55.54 CC.seek :open @14:19:00.00 Forearm.seek :retract @14:19:01.07 Elbow.reconfigure TightElbow @14:19:01.13 Elbow.seek 1 @14:19:03.03 Elbow.reconfigure LimpElbow @14:19:03.10 Forearm.seek 1 @14:19:04.15 Elbow.hold @14:19:04.24 Elbow.reconfigure TightElbow @14:19:04.29 Hand.open @14:19:05.18 Forearm.force -20 @14:19:05.22 Forearm.seek 1 + 347 counts @14:19:05.54 Forearm.reconfigure TightForearm @14:19:05.60 Forearm.seek 1 + 700 counts @14:19:05.91 SE.seek :down @14:19:07.04
Forearm.reconfigure LooseForearm @14:19:07.09 Forearm.seek :retract @14:19:07.74 SE.coast @14:19:08.26
SC.coast @14:19:08.31 Tranferring lysate to PRV @14:19:08.32 CSV.dial :air @14:19:08.68 CS.seek 10ml @14:19:08.95 CSV.dial between air and CTV @14:19:09.32 AIV.dial :bypass @14:19:09.42 Beginning samplePassC @14:19:09.43 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:19:09.52 Hand.open @14:19:09.90 Elbow.seek FlushPuck::Garage @14:19:11.95 Elbow.reconfigure LooseElbow @14:19:12.06 Forearm.seek :garage @14:19:13.12 Hand.close @14:19:14.02 Forearm.seek :retract @14:19:15.10 Elbow.reconfigure TightElbow @14:19:15.15 Elbow.seek CC @14:19:18.07 Elbow.reconfigure LooseElbow @14:19:18.18 Forearm.reconfigure TightForearm @14:19:18.23 Forearm.seek CC @14:19:19.54 CC.seek :closed @14:19:23.79 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.51 @14:19:23.87 Hand.open @14:19:24.71 Forearm.reconfigure LooseForearm @14:19:24.77 Forearm.seek :retract @14:19:25.78 AWV.select :air @14:19:25.80 AIV.dial :bypass @14:19:25.82 PSV.dial PSR @14:19:27.38 Pulling 1.5ml of PSR.flush @14:19:27.39 PSR.select :flush @14:19:27.54 PS.seek 1.5ml @14:19:43.79 delay 5 seconds @14:19:48.81 PSV.dial :waste1 @14:19:49.33 PS.seek 0ml @14:20:05.50 PSV.dial PSR @14:20:06.14 PSR.select :air @14:20:06.27 PS.seek 2.5ml @14:20:32.69 PRV.dial CBV @14:20:32.71
CBV.dial PRV @14:20:33.64 PSV.dial :mixloop @14:20:34.82 CTV.dial :air,avoiding: :intake @14:20:34.84 PTV.dial :PRVmixing @14:20:34.94 PS.seek 0ml @14:21:01.37 PSV.dial :air @14:21:01.90 PS.seek 2.5ml @14:21:28.28 PSV.dial :mixloop @14:21:28.80 PS.seek 0ml @14:21:55.50 PSV.dial :air @14:21:56.02 PS.seek 2.5ml @14:22:22.56 PSV.dial :mixloop @14:22:23.08 PS.seek 0ml @14:22:49.79 Garaging FlushPuck in Collection Clamp @14:22:49.87 Elbow.reconfigure TightElbow @14:22:50.39 Elbow.seek CC @14:22:50.69 Elbow.reconfigure LooseElbow @14:22:50.80 Forearm.seek CC @14:22:51.90 Hand.close @14:22:52.67 CC.seek :open @14:22:57.05 Forearm.seek :retract @14:22:58.14 Elbow.reconfigure TightElbow @14:22:58.20 Elbow.seek :garage @14:23:01.26 Elbow.reconfigure LooseElbow @14:23:01.37 Forearm.reconfigure TightForearm @14:23:01.43 Forearm.seek :garage @14:23:02.56 Hand.open @14:23:03.35 Forearm.reconfigure LooseForearm @14:23:03.40 Forearm.seek :retract @14:23:04.45 Connecting PS to CS to transfer sample @14:23:04.46 CBV.dial AIV @14:23:04.49 AIV.dial :bypass @14:23:04.51 PSV.dial :mixloop @14:23:04.53 PTV.dial :PRVmixing @14:23:04.55 PRV.dial AIV @14:23:04.57 CTV.dial :air,avoiding: :intake @14:23:04.60 Loading lyfil puck from tube 2 into Collection Clamp @14:23:04.84 SC.reconfigure SCtight @14:23:04.90 SC.seek 2 @14:23:07.05 SC.reconfigure SCloose @14:23:07.26 Elbow.reconfigure TightElbow @14:23:07.32 Elbow.seek 2 @14:23:09.53 Forearm.reconfigure TightForearm @14:23:09.59 Forearm.seek 2 + 700 counts @14:23:10.62 SE.reconfigure SEpick @14:23:10.72 SE.seek beamBreak @14:23:13.77 SE.configure SEconfig @14:23:13.93 Elbow.seek 2 @14:23:14.12 Forearm.seek 2 + 300 counts @14:23:14.39 Forearm.reconfigure LooseForearm @14:23:14.46 Hand.close @14:23:15.18 SE.seek :down @14:23:15.39
Forearm.seek :retract @14:23:16.42 Elbow.seek CC @14:23:17.98 SE.coast @14:23:18.08
Elbow.reconfigure LooseElbow @14:23:18.18 Forearm.reconfigure TightForearm @14:23:18.24 Forearm.seek CC @14:23:19.58 CC.seek :closed @14:23:23.87 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.55 @14:23:23.89 Hand.open @14:23:24.73 Forearm.reconfigure LooseForearm @14:23:24.79 Forearm.seek :retract @14:23:25.83 SC.coast @14:23:25.88 CTV.dial CSV,avoiding: :intake @14:23:27.93 CSV.dial CTV @14:23:28.28 CS.seek 5ml @14:23:42.33 delay 1:20 @14:25:02.37 CS.seek 2.5ml @14:25:09.93 delay 40 seconds @14:25:49.96 CS.seek 0ml @14:25:57.65 delay 1:00 @14:26:57.68 PSV.dial :air @14:26:58.18 PS.seek 2.5ml @14:27:24.66 PSV.dial :mixloop @14:27:25.17 delay 3 seconds @14:27:28.19 PSV.dial :air @14:27:28.69 delay 2 seconds @14:27:30.72 PSV.dial :mixloop @14:27:31.23 delay 3 seconds @14:27:34.26 PSV.dial :waste1 @14:27:35.78 PS.seek :empty @14:28:02.47 CBV.dial :waste1 @14:28:03.02 delay 5 seconds @14:28:08.05 CBV.dial AIV @14:28:08.59 delay 10 seconds @14:28:18.63 CBV.dial :waste1 @14:28:19.16 delay 10 seconds @14:28:29.20 CBV.dial AIV @14:28:29.75 delay 10 seconds @14:28:39.78 CTV.dial CSV,avoiding: :intake @14:28:39.79 CBV.dial between AIV and waste1 @14:28:40.18 CSV.dial :air @14:28:40.72 CS.seek 10ml @14:29:07.74 CSV.dial CTV @14:29:08.28 CS.seek 0ml @14:29:35.77 CBV.dial :waste1 @14:29:36.13 delay 10 seconds @14:29:46.17 CTV.dial :air,avoiding: :intake @14:29:47.80 Storing lyfil puck in Collection Clamp to tube 1 @14:29:47.98 SC.reconfigure SCtight @14:29:48.04 SC.seek 1 @14:29:50.16 SC.reconfigure SCloose @14:29:50.38 Elbow.reconfigure TightElbow @14:29:50.44 Elbow.seek 1 @14:29:52.35 Forearm.reconfigure TightForearm @14:29:52.41 Forearm.seek 1 + 700 counts @14:29:53.48 SE.reconfigure SEpick @14:29:53.57 SE.seek beamBreak @14:29:55.78 SE.configure SEconfig @14:29:55.94 Elbow.reconfigure LimpElbow @14:29:56.00 Forearm.reconfigure LooseForearm @14:29:56.07 Forearm.seek 1 @14:29:56.35 Elbow.hold @14:29:56.43 Elbow.reconfigure TightElbow @14:29:56.54 SE.seek 10.09 Pucks below Top Plate @14:29:57.13 Forearm.force -20 @14:29:57.18 Forearm.seek 1 + 350 counts @14:29:57.50 Forearm.reconfigure TightForearm @14:29:57.56 Forearm.seek 1 + 700 counts @14:29:57.96 SE.seek 11.09 Pucks below Top Plate @14:29:58.67 Forearm.reconfigure LooseForearm @14:29:58.73 Forearm.seek :retract @14:29:59.66 Elbow.seek CC @14:30:01.29 Elbow.reconfigure LooseElbow @14:30:01.39 Forearm.seek CC @14:30:02.47 Hand.close @14:30:03.18 CC.seek :open @14:30:08.20 Forearm.seek :retract @14:30:09.24 Elbow.reconfigure TightElbow @14:30:09.30 Elbow.seek 1 @14:30:11.29 Elbow.reconfigure LimpElbow @14:30:11.35 Forearm.seek 1 @14:30:12.35 Elbow.hold @14:30:12.43 Elbow.reconfigure TightElbow @14:30:12.51 Hand.open @14:30:13.41 Forearm.force -20 @14:30:13.46 Forearm.seek 1 + 347 counts @14:30:13.79 Forearm.reconfigure TightForearm @14:30:13.85 Forearm.seek 1 + 700 counts @14:30:14.20 SE.seek :down @14:30:15.33
Forearm.reconfigure LooseForearm @14:30:15.39 Forearm.seek :retract @14:30:15.99 SE.coast @14:30:16.29
SC.coast @14:30:16.34 PSV.dial :air @14:30:17.58 PS.seek 0.499ml @14:30:23.50 delay 3 seconds @14:30:26.53 PSV.dial :mixloop @14:30:27.00 PRV.dial :air @14:30:27.53 delay 3 seconds @14:30:30.56 PRV.dial CBV @14:30:31.08 CBV.dial AIV @14:30:31.62 PRV.dial between CBV and air @14:30:31.99 PRV.dial :air @14:30:32.39 PS.reconfigure PSslow @14:30:32.45 PS.seek 0.599ml @14:30:34.97 PRV.dial :waste2 @14:30:35.82 PS.reconfigure PSconfig @14:30:35.88 PS.seek :empty @14:30:42.85 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:30:42.93 Hand.open @14:30:43.29 Elbow.seek FlushPuck::Garage @14:30:45.29 Elbow.reconfigure LooseElbow @14:30:45.40 Forearm.seek :garage @14:30:46.46 Hand.close @14:30:47.29 Forearm.seek :retract @14:30:48.36 Elbow.reconfigure TightElbow @14:30:48.41 Elbow.seek CC @14:30:51.23 Elbow.reconfigure LooseElbow @14:30:51.34 Forearm.reconfigure TightForearm @14:30:51.39 Forearm.seek CC @14:30:52.71 CC.seek :closed @14:30:57.44 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.52 @14:30:57.59 Hand.open @14:30:58.36 Forearm.reconfigure LooseForearm @14:30:58.41 Forearm.seek :retract @14:30:59.56 Clearing PML for modified lysate @14:30:59.58 CTV.dial :air,avoiding: :intake @14:30:59.60 PRV.dial :waste2 @14:30:59.63 PTV.dial :PRV,avoiding: :puck @14:31:00.49 delay 2 seconds @14:31:02.51 PTV.dial :PRVmixing,avoiding: :puck @14:31:03.35 delay 2 seconds @14:31:05.37 PSV.dial :PML @14:31:05.38 delay 2 seconds @14:31:07.41 PRV.dial :air @14:31:08.24 delay 2 seconds @14:31:10.31 PS.seek 1.499ml @14:31:26.56 delay 5 seconds @14:31:31.59 PS.seek 2.5ml @14:31:42.81 PSV.dial :air @14:31:43.32 PS.seek 0ml @14:32:10.14 mixing lysate with speDil PSV.dial :PML @14:32:10.66 CBV.dial :PRV @14:32:10.68 PRV.dial :air @14:32:10.71 delay 2 seconds @14:32:12.74 PRV.dial :speDil @14:32:13.26 delay 2 seconds @14:32:15.34 PS.reconfigure PSslow1 @14:32:15.39 PS.seek 0.225ml @14:32:39.13 delay 5 seconds @14:32:44.16 PRV.dial CBV @14:32:45.03 delay 2 seconds @14:32:47.10 PS.seek 0.475ml @14:33:13.06 delay 5 seconds @14:33:18.09 PRV.dial :air @14:33:18.62 delay 2 seconds @14:33:20.64 PS.seek 0.965ml @14:34:10.75 delay 10 seconds @14:34:20.79 PRV.dial CBV @14:34:21.31 CTV.dial :air,avoiding: :intake @14:34:21.33 PSV.dial :air @14:34:21.84 PS.reconfigure PSconfig @14:34:21.90 PS.seek 2.5ml @14:34:38.38 delay 3 seconds @14:34:41.41 PSV.dial PTV @14:34:42.24 PTV.dial :PRV,avoiding: :puck @14:34:43.11 PS.seek 0ml @14:35:09.66 PSV.dial :air @14:35:10.51 PS.seek 2.5ml @14:35:37.22 delay 3 seconds @14:35:40.25 PSV.dial PTV @14:35:41.08 PTV.dial :PRV,avoiding: :puck @14:35:41.11 PS.seek 0ml @14:36:07.75 Pushing modified lysate into AIV Injection Loop @14:36:07.77 CBV.dial AIV @14:36:07.79 AIV.dial :bypass @14:36:07.81 PRV.dial CBV @14:36:07.84 PSV.dial :air @14:36:08.68 PS.seek 2.5ml @14:36:35.31 delay 3 seconds @14:36:38.34 PSV.dial :PML @14:36:38.83 PTV.dial :PRVmixing,avoiding: :puck @14:36:39.75 PS.reconfigure PSslow1 @14:36:39.82 PS.seek 1.74ml @14:37:57.53 delay 5 seconds @14:38:02.56 AIV.dial :sample @14:38:03.22 AWV.select :waste @14:38:03.40 PS.reconfigure PSslow2 @14:38:03.46 PS.seek 1.22ml @14:39:51.16 delay 15 seconds @14:40:06.19 AIV.dial :bypass @14:40:06.85 PS.reconfigure PSconfig @14:40:06.91 PS.seek 0ml @14:40:20.10 PSV.dial :air @14:40:20.61 PS.seek 2.5ml @14:40:47.16 PSV.dial :PML @14:40:47.67 PS.seek 0ml @14:41:13.88 AIV.dial :coilToMFB @14:41:13.90 AWV.select :air @14:41:14.08 Extracting Nucleic Acid from AIV @14:41:14.11
Beginning samplePassD @14:41:14.14 SPE.hold 2:46:40,55C @14:41:14.24 ASV.dial :SPEdelivery @14:41:14.31
AWV.select :air @14:41:14.32 AIV.dial :bypass @14:41:14.35 PSV.dial PSR @14:41:14.68 delay 20 seconds @14:41:15.67
Pulling 1.5ml of PSR.killMFB @14:41:15.68 PSR.select :killMFB @14:41:15.83 PS.seek 1.5ml @14:41:32.95 delay 5 seconds @14:41:34.70 primeLysateINT @14:41:34.71 AWV.select :air @14:41:34.73 AIV.dial :coilToMFB @14:41:34.75 ATV.dial AIV @14:41:36.72 AS.reconfigure ASslow1 @14:41:36.77 AS.seek 0.299ml @14:41:37.97
PSV.dial :waste1 @14:41:38.51 PS.seek 0ml @14:41:55.72 PSV.dial PSR @14:41:56.27 PSR.select :air @14:41:56.40 PS.seek 2.5ml @14:42:24.36 PRV.dial CBV @14:42:24.39
CBV.dial PRV @14:42:24.41 PSV.dial :mixloop @14:42:25.64 CTV.dial :air,avoiding: :intake @14:42:25.66 PTV.dial :PRVmixing @14:42:25.69 PS.seek 0ml @14:42:37.46 delay 10 seconds @14:42:47.50 ATV.dial :air2 @14:42:48.43 AS.seek 0.329ml @14:42:54.00
PSV.dial PSR @14:42:54.78 ATV.dial :waste2 @14:42:55.31
Pulling 1.5ml of PSR.flush @14:42:55.32 PSR.select :flush @14:42:55.42 AS.reconfigure ASnorm @14:42:55.48 AS.seek :empty @14:42:55.54
PS.seek 1.5ml @14:43:12.49 Lysate primed! @14:43:12.50 AFV.select :holdingCoil @14:43:12.53 ATV.dial :air2 @14:43:12.76
delay 5 seconds @14:43:13.21 AS.reconfigure ASslow1 @14:43:13.27 AS.seek 0.069ml @14:43:17.78
PSV.dial :waste1 @14:43:18.33 PS.seek 0ml @14:43:27.65 delay 3 seconds @14:43:30.68 ATV.dial :AIV @14:43:31.63 AS.seek 0.469ml @14:43:35.54
PSV.dial PSR @14:43:36.08 PSR.select :air @14:43:36.21 PS.seek 2.5ml @14:44:04.23 PRV.dial CBV @14:44:04.26
CBV.dial PRV @14:44:04.28 PSV.dial :mixloop @14:44:05.50 CTV.dial :air,avoiding: :intake @14:44:05.52 PTV.dial :PRVmixing @14:44:05.55 PS.seek 0ml @14:44:33.50 PSV.dial PSR @14:44:34.78 Pulling 1.5ml of PSR.flush @14:44:34.79 PSR.select :flush @14:44:34.94 PS.seek 1.5ml @14:44:51.92 delay 5 seconds @14:44:52.34 delay 5 seconds @14:44:56.94
PSV.dial :waste1 @14:44:57.41 Solid Phase Extraction Heater at 48.7C @14:44:57.42 ATV.dial ASV @14:44:57.50
PS.seek 0ml @14:44:57.85 ASV.dial :SPEdelivery @14:44:57.94 AS.seek 0.041ml @14:45:14.38
PSV.dial PSR @14:45:14.93 PSR.select :air @14:45:15.06 PS.seek 2.5ml @14:45:43.17 PRV.dial CBV @14:45:43.20
CBV.dial PRV @14:45:43.22 PSV.dial :mixloop @14:45:44.42 CTV.dial :air,avoiding: :intake @14:45:44.43 PTV.dial :PRVmixing @14:45:44.46 PS.seek 0ml @14:46:11.94 PSV.dial PSR @14:46:13.20 Pulling 1.5ml of PSR.flush @14:46:13.21 PSR.select :flush @14:46:13.35 PS.seek 1.5ml @14:46:24.31 delay 10 seconds @14:46:29.90
delay 5 seconds @14:46:34.34 rinseLysateLineINT @14:46:34.35 AFV.select :carrierSolution @14:46:34.47 delay 2 seconds @14:46:34.92
PSV.dial :waste1 @14:46:35.45 PS.seek 0ml @14:46:36.49 AS.reconfigure ASconfig @14:46:36.55 AS.seek 1ml @14:46:51.91
PSV.dial PSR @14:46:52.44 PSR.select :air @14:46:52.57 PS.seek 2.5ml @14:47:19.42 PRV.dial CBV @14:47:19.45
CBV.dial PRV @14:47:19.47 PSV.dial :mixloop @14:47:20.66 CTV.dial :air,avoiding: :intake @14:47:20.68 PTV.dial :PRVmixing @14:47:20.71 PS.reconfigure PSslow @14:47:20.78 PS.seek 1.2ml @14:47:31.95 delay 1 second @14:47:32.97 AFV.select :holdingCoil @14:47:33.08 delay 1 second @14:47:34.10 ATV.dial AIV @14:47:34.67 AIV.dial :coilToMFB @14:47:34.69 AWV.select :waste @14:47:34.81 AS.reconfigure ASslow0 @14:47:34.87 AS.seek 0ml @14:47:47.60
PSV.dial :waste1 @14:47:49.11 PS.reconfigure PSconfig @14:47:49.17 PS.seek 0ml @14:48:02.35 PSV.dial :air @14:48:03.66 PS.seek 2.5ml @14:48:29.97 PSV.dial :mixloop @14:48:30.50 PS.reconfigure PSslow @14:48:30.56 PS.seek 0ml @14:49:15.79 ATV.dial :air2 @14:49:16.49 AS.reconfigure ASslow1 @14:49:16.55 AS.seek 0.05ml @14:49:21.66
PSV.dial :air @14:49:22.19 PS.reconfigure PSconfig @14:49:22.25 PS.seek 2.5ml @14:49:27.00 ATV.dial :AIV @14:49:27.87 AS.seek 0.01ml @14:49:36.20 ATV.dial :waste @14:49:37.93 AS.seek :empty @14:49:40.37 ethanol wash @14:49:40.38 ATV.dial :air2 @14:49:41.70 AS.reconfigure ASslow2 @14:49:41.76 AS.seek 0.009ml @14:49:49.10
PSV.dial :mixloop @14:49:49.62 PS.reconfigure PSslow @14:49:49.68 PS.seek 0ml @14:49:52.17 delay 3 seconds @14:49:55.20 Pulling 0.4ml of Ethanol Wash @14:49:55.21 ATV.dial :ethanol @14:49:56.05 AS.reconfigure ASslow1 @14:49:56.11 AS.seek 0.409ml @14:50:40.83
Cleaning Collection Syringe CSV.dial CSR @14:50:41.42 Pulling 4ml of CSR.flush @14:50:41.43 CSR.select :flush @14:50:41.57 CS.seek 4ml @14:50:53.08 delay 5 seconds @14:50:58.09 CSR.select :air @14:50:58.24 CS.seek 10ml @14:51:14.83 CSV.dial CTV @14:51:14.86
CTV.dial CSV @14:51:16.52 CBV.dial :waste1 @14:51:16.85 delay 3 seconds @14:51:17.08
CS.seek 0ml @14:51:19.88 ATV.dial :air1 @14:51:20.30 delay 3 seconds @14:51:23.37 AS.seek 0.434ml @14:51:28.68 delay 3 seconds @14:51:31.71 ATV.dial :ethanol @14:51:32.35 AS.seek 0.419ml @14:51:35.67 delay 3 seconds @14:51:38.70 ATV.dial ASV @14:51:40.10 AS.seek 0.092ml @14:51:44.79
CSV.dial CSR @14:51:45.40 Pulling 4ml of CSR.flush @14:51:45.41 CSR.select :flush @14:51:45.55 CS.seek 4ml @14:51:57.17 delay 5 seconds @14:52:02.18 CSR.select :air @14:52:02.32 CS.seek 10ml @14:52:18.92 CSV.dial CTV @14:52:18.94
CTV.dial CSV @14:52:19.50 CBV.dial :waste1 @14:52:19.52 CS.seek 0ml @14:52:46.10 delay 3 seconds @14:52:46.63
Garaging FlushPuck in Collection Clamp @14:52:46.72 Elbow.reconfigure TightElbow @14:52:46.77 Elbow.seek CC @14:52:47.02 Elbow.reconfigure LooseElbow @14:52:47.13 Forearm.seek CC @14:52:48.19 Hand.close @14:52:48.95 CC.seek :open @14:52:49.13 ATV.dial :waste2 @14:52:49.93 AS.reconfigure ASconfig @14:52:49.98 AS.seek :empty @14:52:53.38
Forearm.seek :retract @14:52:54.42 Elbow.reconfigure TightElbow @14:52:54.47 Elbow.seek :garage @14:52:55.63 ATV.dial :air2 @14:52:56.05 AS.reconfigure ASslow1 @14:52:56.11 AS.seek 1ml @14:52:57.75
Elbow.reconfigure LooseElbow @14:52:57.86 Forearm.reconfigure TightForearm @14:52:57.91 Forearm.seek :garage @14:52:59.08 Hand.open @14:52:59.89 Forearm.reconfigure LooseForearm @14:52:59.94 Forearm.seek :retract @14:53:01.02 Whole Cell Archive sampling 1500ml @14:53:01.04 Loading wcr puck from tube 2 into Collection Clamp @14:53:01.29 SC.reconfigure SCtight @14:53:01.35 SC.seek 2 @14:53:03.55 SC.reconfigure SCloose @14:53:03.78 Elbow.reconfigure TightElbow @14:53:03.84 Elbow.seek 2 @14:53:06.02 Forearm.reconfigure TightForearm @14:53:06.09 Forearm.seek 2 + 700 counts @14:53:07.14 SE.reconfigure SEpick @14:53:07.24 SE.seek beamBreak @14:53:10.38 SE.configure SEconfig @14:53:10.55 Elbow.seek 2 @14:53:10.75 Forearm.seek 2 + 300 counts @14:53:11.54 Forearm.reconfigure LooseForearm @14:53:11.60 Hand.close @14:53:12.34 SE.seek :down @14:53:12.53 Forearm.seek :retract @14:53:13.58 Elbow.seek CC @14:53:15.25 Elbow.reconfigure LooseElbow @14:53:15.37 Forearm.reconfigure TightForearm @14:53:15.46 SE.coast @14:53:15.48 Forearm.seek CC @14:53:16.83 CC.seek :closed @14:53:21.10 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.44 @14:53:21.11 Hand.open @14:53:21.89 Forearm.reconfigure LooseForearm @14:53:21.95 Forearm.seek :retract @14:53:22.99 SC.coast @14:53:23.05 collectSample 1500ml w/bubble point of 55psi @14:53:23.06 Priming 7ml @14:53:23.08 Intake.select :close @14:53:23.10 Exhaust.select :close @14:53:23.13 SS.hold @14:53:23.35 Calibrating pressure sensors @14:53:23.36 CTV.dial :air @14:53:24.95 SSV.dial :spit,avoiding: :exhaust @14:53:24.97 SS.reconfigure SSfast @14:53:25.05 SS.seek 5ml @14:53:27.75 SSV.dial CBV @14:53:27.77 CBV.dial SSV @14:53:29.43 delay 3.8 seconds @14:53:33.35 SSV.dial :spit @14:53:34.88 SS.seek 0ml @14:53:37.41 SSV.dial CBV @14:53:38.94 CTV.dial :intake @14:53:39.84 SSV.dial CBV @14:53:39.87 CBV.dial SSV @14:53:39.89 Allowing 40s for 7.0ml of remaining 7.0ml @14:53:39.92 Intake.select :open @14:53:42.09 Intake @15.5psia, vacuum from 9.0 to 13.0psi @14:53:42.11 SS.reconfigure SSinhale.with maxSpeed: 150 @14:53:42.19 SS.seek 7ml @14:53:48.66 Stroke completed -- waiting for pressure to equalize ... @14:54:05.13 Intake.select :close @14:54:05.30 SSV.dial :exhaust @14:54:06.18 Exhaust.select :open @14:54:06.31 SS.reconfigure SSexhale @14:54:06.37 SS.seek 0ml @14:54:11.02 Exhaust.select :close @14:54:11.14 Sampling 1500.0ml @14:54:11.16 Intake.select :close @14:54:11.18 Exhaust.select :close @14:54:11.20 SS.reconfigure SSconfig @14:54:11.26 SS.hold @14:54:11.46 CTV.dial :intake @14:54:11.48 SSV.dial CBV @14:54:11.51 CBV.dial SSV @14:54:12.41 Allowing 130s for 25.0ml of remaining 1500.0ml @14:54:12.42 Intake.select :open @14:54:14.61 Intake @15.5psia, vacuum from 9.0 to 13.0psi @14:54:14.63 SS.reconfigure SSinhale.with maxSpeed: 150 @14:54:14.71 SS.seek 25ml @14:54:20.25 Vacuum too high -- waiting up to 40s @14:54:33.30 Intake @16.8psia, vacuum from 10.3 to 14.3psi @14:54:33.32 SS.reconfigure SSinhale.with maxSpeed: 135 @14:54:33.39 SS.seek 25ml @14:54:37.75 Vacuum too high -- waiting up to 40s @14:54:52.64 Intake @17.3psia, vacuum from 10.8 to 14.8psi @14:54:52.66 SS.reconfigure SSinhale.with maxSpeed: 122 @14:54:52.73 SS.seek 25ml @14:54:56.44 Vacuum too high -- waiting up to 40s @14:55:09.94 Intake @17.2psia, vacuum from 10.7 to 14.7psi @14:55:09.96 SS.reconfigure SSinhale.with maxSpeed: 110 @14:55:10.03 SS.seek 25ml @14:55:13.03 Vacuum too high -- waiting up to 40s @14:55:25.42 Intake @16.8psia, vacuum from 10.3 to 14.3psi @14:55:25.44 SS.reconfigure SSinhale.with maxSpeed: 99 @14:55:25.51 SS.seek 25ml @14:55:29.44 Vacuum too high -- waiting up to 40s @14:55:44.58 Intake @17.0psia, vacuum from 10.5 to 14.5psi @14:55:44.60 SS.reconfigure SSinhale.with maxSpeed: 89 @14:55:44.68 SS.seek 25ml @14:55:45.63 Stroke completed -- waiting for pressure to equalize ... @14:55:53.64 Intake.select :close @14:55:53.80 SSV.dial :exhaust @14:55:54.70 Exhaust.select :open @14:55:54.83 SS.reconfigure SSexhale @14:55:54.91 SS.seek 0ml @14:56:04.59 Exhaust.select :close @14:56:04.71 SSV.dial CBV @14:56:04.73 CBV.dial SSV @14:56:05.63 Allowing 130s for 25.0ml of remaining 1475.0ml @14:56:05.65 Intake.select :open @14:56:07.83 Intake @15.9psia, vacuum from 9.4 to 13.4psi @14:56:07.85 SS.reconfigure SSinhale.with maxSpeed: 89 @14:56:07.92 SS.seek 25ml @14:56:11.09 Vacuum too high -- waiting up to 40s @14:56:17.64 delay 5 seconds @14:56:19.06 Intake @15.9psia, vacuum from 9.4 to 13.4psi @14:56:19.08 SS.reconfigure SSinhale.with maxSpeed: 80 @14:56:19.15 SS.seek 25ml @14:56:22.25 Vacuum too high -- waiting up to 40s @14:56:22.71 ATV.dial ASV @14:56:23.74 ASV.dial :SPEdelivery @14:56:23.77 AS.seek 0.02ml @14:56:30.97 Intake @16.1psia, vacuum from 9.6 to 13.6psi @14:56:30.99 SS.reconfigure SSinhale.with maxSpeed: 72 @14:56:31.07 SS.seek 25ml @14:56:34.97 Vacuum too high -- waiting up to 40s @14:56:46.84 Intake @16.4psia, vacuum from 9.9 to 13.9psi @14:56:46.86 SS.reconfigure SSinhale.with maxSpeed: 65 @14:56:46.94 SS.seek 25ml @14:56:51.50 Vacuum too high -- waiting up to 40s @14:57:04.99 Intake @15.9psia, vacuum from 9.4 to 13.4psi @14:57:05.01 SS.reconfigure SSinhale.with maxSpeed: 59 @14:57:05.09 SS.seek 25ml @14:57:10.50 Vacuum too high -- waiting up to 40s @14:57:26.38 Intake @16.2psia, vacuum from 9.7 to 13.7psi @14:57:26.40 SS.reconfigure SSinhale.with maxSpeed: 53 @14:57:26.48 SS.seek 25ml @14:57:31.56 Vacuum too high -- waiting up to 40s @14:57:45.39 Intake @16.3psia, vacuum from 9.8 to 13.8psi @14:57:45.41 SS.reconfigure SSinhale.with maxSpeed: 48 @14:57:45.48 SS.seek 25ml @14:57:51.34 Vacuum too high -- waiting up to 40s @14:58:05.53 Intake @16.3psia, vacuum from 9.8 to 13.8psi @14:58:05.55 SS.reconfigure SSinhale.with maxSpeed: 43 @14:58:05.63 SS.seek 25ml @14:58:09.91 Stroke completed -- waiting for pressure to equalize ... @14:58:15.66 SS.reconfigure SSconfig @14:58:15.72 SS.hold @14:58:15.85 Intake.select :close @14:58:16.00 ShallowSampler::Clogged in WCR -- progress too slow after 50.0ml @14:58:16.02 SSV.dial :exhaust @14:58:16.91 Exhaust.select :open @14:58:17.05 SS.reconfigure SSexhale @14:58:17.12 SS.seek 0ml @14:58:27.01 Exhaust.select :close @14:58:27.13 Sampled 50.0ml @14:58:27.15 Drying puck before fixing for 4:00 @14:58:27.17 CBV.dial :waste2b @14:58:28.08 delay 1:00 @14:59:28.11 CSV.dial :air @14:59:28.65 CS.reconfigure CSfast @14:59:28.71 CS.seek 10ml @14:59:41.24 delay 5 seconds @14:59:46.26 ATV.dial :air2 @14:59:47.34 AS.seek 0.52ml @14:59:55.71 Pumping wcr puck from tube 2 up to 55psi... @14:59:55.72 CBV.dial SSV @14:59:57.20 CBV.dial between CSV and waste2b @14:59:58.34 CSV.dial between air and CTV @14:59:58.73 CS.seek 7.377ml @15:00:06.61 CTV.dial CSV,avoiding: :air @15:00:06.64 CSV.dial CTV @15:00:07.58 CS.reconfigure CSpressurize @15:00:07.64 CS.seek 0ml @15:00:24.44 CBV.dial :waste2b @15:00:24.83 CS.reconfigure CSconfig @15:00:24.89 CS.seek :empty @15:00:28.98 delay 1:00 @15:01:28.23 delay 5 seconds @15:01:29.01 CTV.dial :air,avoiding: :intake @15:01:30.60 Fix #1 of 2 (with rnal) for 2:00 @15:01:30.62 CSV.dial CSR @15:01:31.20 Pulling 2ml of CSR.rnal @15:01:31.21 CSR.select :rnal @15:01:31.36 CS.seek 2ml @15:01:33.25 ATV.dial ASV @15:01:34.26 ASV.dial :wasteR @15:01:34.74 AS.seek 0.3ml @15:01:37.83 delay 5 seconds @15:01:42.86 CSR.select :air @15:01:42.98 CS.reconfigure CSfast @15:01:43.04 CS.seek 10ml @15:02:04.74 Pumping wcr puck from tube 2 up to 55psi... @15:02:04.75 CBV.dial SSV @15:02:06.21 CBV.dial between CSV and waste2b @15:02:07.36 CSV.dial between air and CTV @15:02:08.09 CS.seek 7.207ml @15:02:16.55 CTV.dial CSV,avoiding: :air @15:02:16.57 CSV.dial CTV @15:02:18.21 CS.reconfigure CSpressurize @15:02:18.28 CS.seek 0ml @15:02:19.26 delay 20 seconds @15:02:36.53 delay 2:00 @15:02:39.29 AS.seek 0.07ml @15:03:25.82 delay 20 seconds @15:03:45.86 ATV.dial :waste2 @15:03:46.64 AS.reconfigure ASconfig @15:03:46.70 AS.seek :empty @15:03:51.09 Elution @15:03:51.10 ATV.dial :air1 @15:03:52.27 AS.reconfigure ASslow1 @15:03:52.33 AS.seek 0.049ml @15:04:02.68 delay 3 seconds @15:04:05.71 ATV.dial :ElutionWater @15:04:06.41 AS.seek 0.109ml @15:04:18.73 delay 3 seconds @15:04:21.76 ATV.dial ASV,via: :air2 @15:04:23.91 ASV.dial :SPEelution @15:04:24.53 AFV.select :carrierSolution @15:04:24.69 AS.reconfigure ASnorm @15:04:24.75 AS.seek 0.759ml @15:04:36.57 CBV.dial :waste2b @15:04:36.96 CS.reconfigure CSconfig @15:04:37.02 CS.seek :empty @15:04:39.36 delay 1:00 @15:04:57.84 delay 3 seconds @15:05:00.86 AFV.select :holdingCoil @15:05:01.01 AS.reconfigure ASslow1 @15:05:01.07 AS.seek 0.474ml @15:05:39.39 CTV.dial :air,avoiding: :intake @15:05:40.98 Fix #2 of 2 (with rnal) for 2:00 @15:05:41.00 CSV.dial CSR @15:05:41.57 Pulling 2ml of CSR.rnal @15:05:41.58 CSR.select :rnal @15:05:41.72 CS.seek 2ml @15:05:47.90 delay 5 seconds @15:05:52.93 CSR.select :air @15:05:53.06 CS.reconfigure CSfast @15:05:53.12 CS.seek 10ml @15:05:58.63 delay 5 seconds @15:06:03.71 AS.reconfigure ASslow2 @15:06:03.77 AS.seek 0.439ml @15:06:14.87 Pumping wcr puck from tube 2 up to 55psi... @15:06:14.88 CBV.dial SSV @15:06:16.32 CBV.dial between CSV and waste2b @15:06:17.46 CSV.dial between air and CTV @15:06:18.68 CS.seek 7.207ml @15:06:26.87 CTV.dial CSV,avoiding: :air @15:06:26.89 CSV.dial CTV @15:06:28.52 CS.reconfigure CSpressurize @15:06:28.59 CS.seek 0ml @15:06:39.28 delay 1:00 @15:06:45.85 delay 2:00 @15:07:39.31 pushing eluate to holding coil @15:07:39.39 AS.seek 0.279ml @15:08:45.88 CBV.dial :waste2b @15:08:46.28 CS.reconfigure CSconfig @15:08:46.34 CS.seek :empty @15:08:49.52 delay 1:00 @15:09:49.56 CTV.dial :air,avoiding: :intake @15:09:51.20 Storing wcr puck in Collection Clamp to tube 1 @15:09:51.38 SC.reconfigure SCtight @15:09:51.44 SC.seek 1 @15:09:53.59 SC.reconfigure SCloose @15:09:53.82 Elbow.reconfigure TightElbow @15:09:53.88 Elbow.seek 1 @15:09:55.88 Forearm.reconfigure TightForearm @15:09:55.97 Forearm.seek 1 + 700 counts @15:09:57.11 SE.reconfigure SEpick @15:09:57.20 SE.seek beamBreak @15:09:59.38 SE.configure SEconfig @15:09:59.55 Elbow.reconfigure LimpElbow @15:09:59.62 Forearm.reconfigure LooseForearm @15:09:59.68 Forearm.seek 1 @15:10:00.01 Elbow.hold @15:10:00.10 Elbow.reconfigure TightElbow @15:10:00.21 SE.seek 11.1 Pucks below Top Plate @15:10:00.67 Forearm.force -20 @15:10:00.73 Forearm.seek 1 + 344 counts @15:10:01.04 Forearm.reconfigure TightForearm @15:10:01.11 Forearm.seek 1 + 700 counts @15:10:01.41 SE.seek 12.1 Pucks below Top Plate @15:10:02.09 Forearm.reconfigure LooseForearm @15:10:02.15 Forearm.seek :retract @15:10:03.11 Elbow.seek CC @15:10:04.68 Elbow.reconfigure LooseElbow @15:10:04.80 Forearm.seek CC @15:10:05.88 Hand.close @15:10:06.60 CC.seek :open @15:10:11.12 Forearm.seek :retract @15:10:12.19 Elbow.reconfigure TightElbow @15:10:12.25 Elbow.seek 1 @15:10:14.63 Elbow.reconfigure LimpElbow @15:10:14.70 Forearm.seek 1 @15:10:15.77 Elbow.hold @15:10:15.85 Elbow.reconfigure TightElbow @15:10:15.92 Hand.open @15:10:16.83 Forearm.force -20 @15:10:16.89 Forearm.seek 1 + 346 counts @15:10:17.22 Forearm.reconfigure TightForearm @15:10:17.29 Forearm.seek 1 + 700 counts @15:10:17.59 SE.seek :down @15:10:18.72 Forearm.reconfigure LooseForearm @15:10:18.79 Forearm.seek :retract @15:10:19.25 SE.coast @15:10:19.73 SC.coast @15:10:19.79 Starting finalPurge @15:10:19.81 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:10:19.90 Hand.open @15:10:20.22 Elbow.seek FlushPuck::Garage @15:10:20.88 delay 30 seconds @15:10:22.37
Elbow.reconfigure LooseElbow @15:10:22.48 Forearm.seek :garage @15:10:23.51 Hand.close @15:10:24.33 Forearm.seek :retract @15:10:25.39 Elbow.reconfigure TightElbow @15:10:25.45 Elbow.seek CC @15:10:28.38 Elbow.reconfigure LooseElbow @15:10:28.49 Forearm.reconfigure TightForearm @15:10:28.55 Forearm.seek CC @15:10:29.89 CC.seek :closed @15:10:34.17 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.46 @15:10:34.25 Hand.open @15:10:35.03 Forearm.reconfigure LooseForearm @15:10:35.08 Forearm.seek :retract @15:10:36.13 Flushing the Collection Valve::Manifold @15:10:36.26 CSV.dial CSR @15:10:36.83 Pulling 5ml of CSR.flush @15:10:36.84 CSR.select :flush @15:10:36.97 CS.seek 5ml @15:10:50.90 delay 5 seconds @15:10:50.93 ATV.dial :waste,via: :air3 @15:10:52.69 AS.reconfigure ASconfig @15:10:52.74 AS.seek :empty @15:10:55.91
CSR.select :air @15:10:56.09 CS.reconfigure CSfast @15:10:56.15 CS.seek 7.5ml @15:11:04.11 CSV.dial :waste @15:11:05.68 CS.reconfigure CSconfig @15:11:05.74 CS.seek 0ml @15:11:09.13 Parking Bleach in LysateSource line and SPEcolumn @15:11:09.14 ATV.dial :air3 @15:11:10.02 AS.reconfigure ASslow1 @15:11:10.07 AS.seek 0.029ml @15:11:16.51 ATV.dial :ElutionWater @15:11:17.14 AS.seek 0.059ml @15:11:23.45 ATV.dial :air3 @15:11:24.08 AS.seek 0.069ml @15:11:26.28
Flushing lower manifold's waste1 port @15:11:26.43 CSV.dial :flush @15:11:26.46 delay 2 seconds @15:11:27.00
CS.reconfigure CSfast @15:11:27.06 CS.seek 5ml @15:11:28.49 ATV.dial :bleach @15:11:28.97 AS.seek 0.769ml @15:11:41.02
delay 5 seconds @15:11:46.05 CSV.dial :air @15:11:46.62 CS.seek 7ml @15:11:52.87 CSV.dial CTV @15:11:52.90
CTV.dial CSV @15:11:54.53 CBV.dial :waste1 @15:11:55.45 CS.seek 0ml @15:12:14.59 Flushing lower manifold's waste2b port @15:12:14.72 CSV.dial :flush @15:12:15.62 CS.seek 5ml @15:12:29.49 delay 5 seconds @15:12:34.52 CSV.dial :air @15:12:35.13 CS.seek 7ml @15:12:41.25 CSV.dial CTV @15:12:41.27
CTV.dial CSV @15:12:41.81 CBV.dial :waste2b @15:12:42.72 CS.seek 0ml @15:13:01.75 Flushing lower manifold's waste1 port @15:13:01.76 CSV.dial CSR @15:13:02.34 Pulling 4ml of CSR.mfbkill @15:13:02.35 CSR.select :mfbkill @15:13:02.50 CS.reconfigure CSconfig @15:13:02.55 CS.seek 4ml @15:13:14.15 delay 5 seconds @15:13:19.17 CSR.select :air @15:13:19.30 CS.reconfigure CSfast @15:13:19.37 CS.seek 10ml @15:13:35.93 CSV.dial CTV @15:13:35.95
CTV.dial CSV @15:13:36.51 CBV.dial :waste1 @15:13:37.42 CS.seek 0ml @15:13:50.18 delay 3 seconds @15:13:53.21 ATV.dial :air3 @15:13:53.79 delay 3 seconds @15:13:56.86 AS.seek 0.794ml @15:14:02.18 delay 3 seconds @15:14:04.37
Flushing lower manifold's waste2b port @15:14:04.38 CSV.dial CSR @15:14:04.99 Pulling 4ml of CSR.mfbkill @15:14:05.00 CSR.select :mfbkill @15:14:05.15 CS.reconfigure CSconfig @15:14:05.20 ATV.dial :bleach @15:14:05.24
CS.seek 4ml @15:14:05.89 AS.seek 0.779ml @15:14:09.24 delay 3 seconds @15:14:12.27 ATV.dial :AIV @15:14:13.57 AS.reconfigure ASconfig @15:14:13.63 AS.seek 0.015ml @15:14:16.62
delay 5 seconds @15:14:21.64 CSR.select :air @15:14:21.76 CS.reconfigure CSfast @15:14:21.83 CS.seek 10ml @15:14:38.40 CSV.dial CTV @15:14:38.42
CTV.dial CSV @15:14:38.98 CBV.dial :waste2b @15:14:40.37 CS.seek 0ml @15:14:57.85 ATV.dial :waste @15:14:59.41 AS.seek :empty @15:15:00.60 ATV.dial :air3 @15:15:01.30 AS.reconfigure ASslow1 @15:15:01.35 AS.seek 0.009ml @15:15:03.63 delay 2 seconds @15:15:05.66 ATV.dial :bleach @15:15:06.13 AS.seek 0.579ml @15:15:07.12
Killing upper manifold @15:15:07.13 CSV.dial CSR @15:15:07.72 Pulling 4ml of CSR.mfbkill @15:15:07.73 CSR.select :mfbkill @15:15:07.87 CS.reconfigure CSconfig @15:15:07.92 CS.seek 4ml @15:15:19.28 delay 5 seconds @15:15:24.30 CSR.select :air @15:15:24.43 CS.reconfigure CSfast @15:15:24.50 CS.seek 10ml @15:15:40.94 CSV.dial CBV @15:15:40.96
CBV.dial CSV @15:15:42.25 CTV.dial :air @15:15:43.83 CS.seek 0ml @15:16:10.68 delay 7 seconds @15:16:17.72 CS.seek 3ml @15:16:26.40 CSV.dial :waste @15:16:26.92 CS.seek 0ml @15:16:35.58 Flushing upper manifold @15:16:35.72 CSV.dial :flush @15:16:36.26 CS.seek 5ml @15:16:50.18 delay 5 seconds @15:16:55.21 CSV.dial :air @15:16:55.79 CS.seek 7ml @15:17:01.04 delay 3 seconds @15:17:01.93 CSV.dial CBV @15:17:01.95
CBV.dial CSV @15:17:03.19 CTV.dial :air @15:17:03.22 CS.seek 0ml @15:17:04.07 ATV.dial :air3 @15:17:04.64 delay 3 seconds @15:17:07.71 AS.seek 0.604ml @15:17:13.03 delay 3 seconds @15:17:16.06 ATV.dial :bleach @15:17:16.74 AS.seek 0.589ml @15:17:19.99 delay 3 seconds @15:17:22.27
delay 7 seconds @15:17:23.02 ATV.dial :ASV @15:17:24.07 ASV.dial :wasteR @15:17:24.69 AS.seek 0ml @15:17:29.30
CS.seek 3ml @15:17:37.96 CSV.dial :waste @15:17:38.51 CS.seek 0ml @15:17:47.26 CSV.dial :flush @15:17:47.78 CS.seek 3ml @15:17:56.58 delay 5 seconds @15:18:01.61 CSV.dial :air @15:18:02.22 CS.seek 5ml @15:18:08.40 CSV.dial :air @15:18:08.43 CS.seek 0ml @15:18:22.37 delay 7 seconds @15:18:29.41 CS.seek 3ml @15:18:38.05 CSV.dial :waste @15:18:38.95 CS.seek 0ml @15:18:47.68 Flushing lower manifold's waste1 port @15:18:47.82 CSV.dial :flush @15:18:48.38 CS.seek 5ml @15:19:02.21 delay 5 seconds @15:19:07.24 CSV.dial :air @15:19:07.83 CS.seek 7ml @15:19:13.93 CSV.dial CTV @15:19:13.97
CTV.dial CSV @15:19:15.60 CBV.dial :waste1 @15:19:16.63 CS.seek 0ml @15:19:23.51 delay 1:00 @15:19:35.75
Flushing lower manifold's waste2b port @15:19:35.88 CSV.dial :flush @15:19:36.78 CS.seek 5ml @15:19:50.68 delay 5 seconds @15:19:55.71 CSV.dial :air @15:19:56.31 CS.seek 7ml @15:20:02.50 CSV.dial CTV @15:20:02.52
CTV.dial CSV @15:20:03.06 CBV.dial :waste2b @15:20:03.97 CS.seek 0ml @15:20:23.06 Garaging FlushPuck in Collection Clamp @15:20:23.14 Elbow.reconfigure TightElbow @15:20:23.20 Elbow.seek CC @15:20:23.50 Elbow.reconfigure LooseElbow @15:20:23.61 Forearm.seek CC @15:20:23.66 AFV.select :carrierSolution @15:20:23.78 delay 2 seconds @15:20:24.66
Hand.close @15:20:25.48 CC.seek :open @15:20:25.80 AS.reconfigure ASconfig @15:20:25.86 AS.seek 1ml @15:20:29.86
Forearm.seek :retract @15:20:30.94 Elbow.reconfigure TightElbow @15:20:30.99 Elbow.seek :garage @15:20:34.25 Elbow.reconfigure LooseElbow @15:20:34.36 Forearm.reconfigure TightForearm @15:20:34.42 Forearm.seek :garage @15:20:35.57 Hand.open @15:20:36.42 Forearm.reconfigure LooseForearm @15:20:36.47 Forearm.seek :retract @15:20:37.51 Completed [mbaPhase] begun 13:37:23 @15:20:37.53 Extending 20invasivedeploymfbtest until completion of MFBPCR @15:21:23.60 delay 1 second @15:21:24.62 AFV.select :holdingCoil @15:21:24.73 delay 1 second @15:21:25.75 ATV.dial ASV @15:21:25.76 ASV.dial :wasteR @15:21:25.79 AS.reconfigure ASslow1 @15:21:25.84 AS.seek 0ml @15:24:47.21 delay 20 seconds @15:25:07.25 ATV.dial :air3 @15:25:08.80 AS.seek 0.59ml @15:27:07.73 delay 3 seconds @15:27:10.76 ATV.dial :ASV @15:27:12.20 ASV.dial :wasteR @15:27:12.29 AS.seek 0.09ml @15:28:53.21 delay 30 seconds @15:29:23.25 ATV.dial :extSrc @15:29:24.04 delay 3 seconds @15:29:27.07 ATV.dial :ElutionWater @15:29:28.19 AS.seek 0.12ml @15:29:34.51 ATV.dial :air3 @15:29:35.13 AS.seek 0.13ml @15:29:37.51 delay 2 seconds @15:29:39.54 ATV.dial :bleach @15:29:40.02 AS.seek 0.23ml @15:30:00.38 delay 3 seconds @15:30:03.41 ATV.dial :air3 @15:30:03.98 delay 3 seconds @15:30:07.06 AS.seek 0.255ml @15:30:12.41 delay 3 seconds @15:30:15.44 ATV.dial :bleach @15:30:16.07 AS.seek 0.24ml @15:30:19.34 delay 3 seconds @15:30:22.37 ATV.dial :ASV @15:30:23.42 ASV.dial :SPEdelivery @15:30:23.81 AFV.select :carrierSolution @15:30:23.93 delay 2 seconds @15:30:25.95 AS.reconfigure ASconfig @15:30:26.00 AS.seek 0.5ml @15:30:41.27 delay 1 second @15:30:42.29 AFV.select :holdingCoil @15:30:42.44 AS.reconfigure ASslow1 @15:30:42.50 AS.seek 0.17ml @15:31:49.18 SPE.hold 2:46:40,95C @15:31:49.25 delay 3:00 @15:34:49.75 AFV.select :carrierSolution @15:34:49.87 delay 2 seconds @15:34:51.89 AS.reconfigure ASconfig @15:34:51.95 AS.seek 1ml @15:35:39.96 delay 1 second @15:35:40.98 AFV.select :holdingCoil @15:35:41.09 delay 1 second @15:35:42.11 ASV.dial :wasteD @15:35:43.22 AS.seek 0ml @15:36:41.01 Priming Eluate @15:36:41.02 AFV.select :carrierSolution @15:36:41.15 delay 2 seconds @15:36:43.17 AS.seek 0.5ml @15:37:12.20 delay 1 second @15:37:13.22 AFV.select :holdingCoil @15:37:13.33 ATV.dial ASV @15:37:13.34 ASV.dial :SPErecovery @15:37:13.96 Swish #1 of 2 @15:37:14.05 AS.reconfigure ASslow0 @15:37:14.11 AS.seek 0.6ml @15:37:24.54 AS.seek 0.5ml @15:37:34.90 Swish #2 of 2 @15:37:34.99 AS.seek 0.6ml @15:37:45.46 AS.seek 0.5ml @15:37:55.86 delay 3 seconds @15:37:59.10 AS.reconfigure ASslow1 @15:37:59.30 AS.seek 0.684ml @15:38:36.68 delay 3 seconds @15:38:39.71 Eluate primed @15:38:39.73 ATV.dial :waste2 @15:38:40.46 AS.reconfigure ASnorm @15:38:40.54 AS.seek 0ml @15:39:15.34 SPE.coast @15:39:15.44 ASV.dial :SPErecovery @15:39:15.47 Setting up PCR for OM -- writing /var/log/esp/OM20may0715h300ml.pcr @15:39:15.52 Building PCR Slug from ASV with MM3 mix, PP5 primer @15:39:15.54 Priming with carrier @15:39:15.57 ATV.dial ARV @15:39:16.60 ARV.dial :carrier @15:39:16.98 AS.seek 0.5ml @15:39:42.60 AFV.select :carrierSolution @15:39:42.73 delay 2 seconds @15:39:44.76 AS.reconfigure ASconfig @15:39:44.84 AS.seek 1ml @15:40:13.88 delay 1 second @15:40:14.90 AFV.select :holdingCoil @15:40:15.01 delay 1 second @15:40:16.04 ATV.dial :waste @15:40:17.58 AS.reconfigure ASnorm @15:40:17.66 AS.seek :empty @15:41:08.43 AFV.select :carrierSolution @15:41:08.56 delay 2 seconds @15:41:10.59 AS.reconfigure ASconfig @15:41:10.66 AS.seek 0.5ml @15:41:39.80 delay 1 second @15:41:40.82 AFV.select :holdingCoil @15:41:40.94 Building Slug @15:41:40.95 ATV.dial :ARV @15:41:42.63 delay 2 seconds @15:41:44.66 ARV.dial :air1 @15:41:45.07 delay 2 seconds @15:41:47.15 AS.reconfigure ASslow2 @15:41:47.23 AS.seek 0.506ml @15:41:53.50 delay 2 seconds @15:41:55.53 ARV.dial :MM3 @15:41:56.61 delay 2 seconds @15:41:58.64 Adding MM3 @15:41:58.74 AS.seek 0.524ml @15:42:17.10 delay 25 seconds @15:42:42.13 ARV.dial :PP5 @15:42:42.53 Adding PP5 @15:42:42.55 delay 2 seconds @15:42:44.64 AS.seek 0.53ml @15:42:50.94 delay 10 seconds @15:43:00.97 ARV.dial :air3 @15:43:01.38 adding ARV air @15:43:01.40 delay 2 seconds @15:43:03.49 AS.seek 0.536ml @15:43:09.89 delay 5 seconds @15:43:14.92 ARV.dial :carrier @15:43:16.22 delay 7 seconds @15:43:23.31 AS.seek 0.594ml @15:44:21.85 delay 5 seconds @15:44:26.88 ATV.dial :ASV @15:44:27.30 delay 2 seconds @15:44:29.39 AS.seek 0.6ml @15:44:35.61 delay 4 seconds @15:44:39.64 ATV.dial ARV @15:44:40.32 delay 2 seconds @15:44:42.35 ARV.dial :carrier @15:44:42.37 delay 2 seconds @15:44:44.47 AS.seek 0.65ml @15:45:34.99 delay 4 seconds @15:45:39.02 ATV.dial PCR @15:45:39.64 ASV.dial :waste1 @15:45:41.02 ARV.dial :air1 @15:45:41.44 Positioning Slug by 0.324ml @15:45:41.56 AS.seek 0.326ml @15:51:07.74 delay 5 seconds @15:51:12.77 ATV.dial ARV @15:51:13.56 ASV.dial :PCRlock @15:51:13.97 delay 5 seconds @15:51:18.99 Running OM Profile60C350 thermocycling @15:51:19.01 PCR.program Profile60C350 @15:51:19.13 Profile60C350 should complete in < 2:02:45 @15:51:19.14 PCR.run 2:38:26.25 @17:28:58.92 PCR.off @17:29:00.20 PCR for OM completed -- closed /var/log/esp/OM20may0715h300ml.pcr @17:29:00.29 Cleaning PCR @17:29:00.30 AFV.select :carrierSolution @17:29:00.43 delay 2 seconds @17:29:02.46 AS.reconfigure ASconfig @17:29:02.53 AS.seek 0.5ml @17:29:12.83 delay 1 second @17:29:13.85 AFV.select :holdingCoil @17:29:13.96 ASV.dial :waste1 @17:29:14.38 ATV.dial :air3 @17:29:15.71 AS.reconfigure ASslow1 @17:29:15.78 AS.seek 0.51ml @17:29:18.13 delay 2 seconds @17:29:20.15 ATV.dial :bleach @17:29:20.81 AS.seek 0.81ml @17:30:21.41 delay 3 seconds @17:30:24.44 ATV.dial :air3 @17:30:25.02 delay 3 seconds @17:30:28.11 AS.seek 0.835ml @17:30:33.43 delay 3 seconds @17:30:36.45 ATV.dial :bleach @17:30:37.69 AS.seek 0.82ml @17:30:41.17 delay 3 seconds @17:30:44.20 ARV.dial :waste @17:30:44.83 delay 3 seconds @17:30:47.86 ATV.dial :ARV @17:30:48.78 AS.reconfigure ASconfig @17:30:48.85 AS.seek 0.47ml @17:31:09.30 AFV.select :carrierSolution @17:31:09.42 delay 2 seconds @17:31:11.46 AS.seek 1ml @17:31:42.22 delay 1 second @17:31:43.24 AFV.select :holdingCoil @17:31:43.36 delay 1 second @17:31:44.39 AS.reconfigure ASfast @17:31:44.46 AS.seek 0ml @17:32:11.83 AFV.select :carrierSolution @17:32:11.96 delay 2 seconds @17:32:13.99 AS.reconfigure ASconfig @17:32:14.06 AS.seek 1ml @17:33:11.83 delay 1 second @17:33:12.85 AFV.select :holdingCoil @17:33:12.97 delay 1 second @17:33:14.00 AS.reconfigure ASfast @17:33:14.07 AS.seek 0ml @17:33:41.57 AFV.select :carrierSolution @17:33:41.71 delay 2 seconds @17:33:43.74 AS.reconfigure ASconfig @17:33:43.81 AS.seek 1ml @17:34:41.61 delay 1 second @17:34:42.63 AFV.select :holdingCoil @17:34:42.75 delay 1 second @17:34:43.78 AS.reconfigure ASfast @17:34:43.85 AS.seek 0ml @17:35:11.22 ARV.dial between carrier and air1 @17:35:11.74 ATV.dial :air3 @17:35:13.07 AS.reconfigure ASslow1 @17:35:13.14 AS.seek 0.01ml @17:35:15.46 delay 2 seconds @17:35:17.48 ATV.dial :bleach @17:35:18.14 AS.seek 0.81ml @17:37:59.30 delay 3 seconds @17:38:02.33 ATV.dial :air3 @17:38:02.91 delay 3 seconds @17:38:06.00 AS.seek 0.835ml @17:38:11.35 delay 3 seconds @17:38:14.38 ATV.dial :bleach @17:38:15.04 AS.seek 0.82ml @17:38:18.38 delay 3 seconds @17:38:21.41 ATV.dial PCR @17:38:21.82 AS.reconfigure ASconfig @17:38:21.88 AS.seek :empty @17:39:09.36 PCR.program :bleachProfile @17:39:09.42 PCR program should complete in < 2:45 @17:39:09.43 PCR.run 10:00 @17:42:05.21 PCR.off @17:42:05.25 AFV.select :carrierSolution @17:42:05.37 delay 2 seconds @17:42:07.41 AS.seek 1ml @17:43:05.25 delay 1 second @17:43:06.27 AFV.select :holdingCoil @17:43:06.39 delay 1 second @17:43:07.42 AS.reconfigure ASfast @17:43:07.49 AS.seek 0ml @17:43:34.85 delay 3 seconds @17:43:37.88 AFV.select :carrierSolution @17:43:38.00 delay 2 seconds @17:43:40.03 AS.reconfigure ASconfig @17:43:40.10 AS.seek 1ml @17:44:37.86 delay 1 second @17:44:38.88 AFV.select :holdingCoil @17:44:39.00 delay 1 second @17:44:40.03 AS.reconfigure ASfast @17:44:40.10 AS.seek 0ml @17:45:07.47 delay 3 seconds @17:45:10.50 AFV.select :carrierSolution @17:45:10.62 delay 2 seconds @17:45:12.65 AS.reconfigure ASconfig @17:45:12.73 AS.seek 1ml @17:46:10.55 delay 1 second @17:46:11.57 AFV.select :holdingCoil @17:46:11.69 delay 1 second @17:46:12.72 AS.reconfigure ASfast @17:46:12.79 AS.seek 0ml @17:46:40.17 delay 3 seconds @17:46:43.20 AFV.select :carrierSolution @17:46:43.32 delay 2 seconds @17:46:45.35 AS.reconfigure ASconfig @17:46:45.45 AS.seek 1ml @17:47:43.28 delay 1 second @17:47:44.30 AFV.select :holdingCoil @17:47:44.42 delay 1 second @17:47:45.45 AS.reconfigure ASfast @17:47:45.52 AS.seek 0ml @17:48:12.89 delay 3 seconds @17:48:15.92 AFV.select :carrierSolution @17:48:16.04 delay 2 seconds @17:48:18.07 AS.reconfigure ASconfig @17:48:18.14 AS.seek 1ml @17:49:15.94 delay 1 second @17:49:16.96 AFV.select :holdingCoil @17:49:17.08 delay 1 second @17:49:18.61 AS.reconfigure ASnorm @17:49:18.76 AS.seek 0ml @17:50:09.51 Setting up PCR for CG -- writing /var/log/esp/CG20may0717h300ml.pcr @17:50:09.53 Building PCR Slug from ASV with MM1 mix, PP2 primer @17:50:09.56 Priming with carrier @17:50:09.59 ATV.dial ARV @17:50:10.24 ARV.dial :carrier @17:50:10.60 AS.seek 0.5ml @17:50:36.21 AFV.select :carrierSolution @17:50:36.34 delay 2 seconds @17:50:38.37 AS.reconfigure ASconfig @17:50:38.44 AS.seek 1ml @17:51:07.50 delay 1 second @17:51:08.53 AFV.select :holdingCoil @17:51:08.65 delay 1 second @17:51:09.68 ATV.dial :waste @17:51:11.38 AS.reconfigure ASnorm @17:51:11.46 AS.seek :empty @17:52:02.25 AFV.select :carrierSolution @17:52:02.38 delay 2 seconds @17:52:04.41 AS.reconfigure ASconfig @17:52:04.48 AS.seek 0.5ml @17:52:33.58 delay 1 second @17:52:34.60 AFV.select :holdingCoil @17:52:34.72 Building Slug @17:52:34.73 ATV.dial :ARV @17:52:36.41 delay 2 seconds @17:52:38.44 ARV.dial :air1 @17:52:38.86 delay 2 seconds @17:52:40.95 AS.reconfigure ASslow2 @17:52:41.02 AS.seek 0.506ml @17:52:47.27 delay 2 seconds @17:52:49.30 ARV.dial :MM1 @17:52:50.00 delay 2 seconds @17:52:52.03 Adding MM1 @17:52:52.12 AS.seek 0.524ml @17:53:10.53 delay 25 seconds @17:53:35.56 ARV.dial :PP2 @17:53:35.97 Adding PP2 @17:53:35.99 delay 2 seconds @17:53:38.08 AS.seek 0.53ml @17:53:44.63 delay 10 seconds @17:53:54.66 ARV.dial :air2 @17:53:55.05 adding ARV air @17:53:55.07 delay 2 seconds @17:53:57.16 AS.seek 0.536ml @17:54:03.47 delay 5 seconds @17:54:08.50 ARV.dial :carrier @17:54:09.80 delay 7 seconds @17:54:16.89 AS.seek 0.594ml @17:55:15.55 delay 5 seconds @17:55:20.58 ATV.dial :ASV @17:55:20.99 delay 2 seconds @17:55:23.08 AS.seek 0.6ml @17:55:29.39 delay 4 seconds @17:55:33.42 ATV.dial ARV @17:55:33.99 delay 2 seconds @17:55:36.02 ARV.dial :carrier @17:55:36.04 delay 2 seconds @17:55:38.14 AS.seek 0.65ml @17:56:28.69 delay 4 seconds @17:56:32.71 ATV.dial PCR @17:56:33.35 ASV.dial :waste1 @17:56:33.37 ARV.dial :air1 @17:56:33.81 Positioning Slug by 0.324ml @17:56:33.92 AS.seek 0.326ml @18:01:59.86 delay 5 seconds @18:02:04.89 ATV.dial ARV @18:02:05.69 ASV.dial :PCRlock @18:02:06.11 delay 5 seconds @18:02:11.14 Running CG Profile60C300 thermocycling @18:02:11.16 PCR.program Profile60C300 @18:02:11.28 Profile60C300 should complete in < 1:59:25 @18:02:11.30 PCR.run 2:34:16.25 @19:38:53.33 PCR.off @19:38:54.02 PCR for CG completed -- closed /var/log/esp/CG20may0717h300ml.pcr @19:38:54.11 Cleaning PCR @19:38:54.12 AFV.select :carrierSolution @19:38:54.26 delay 2 seconds @19:38:56.29 AS.reconfigure ASconfig @19:38:56.36 AS.seek 0.5ml @19:39:06.69 delay 1 second @19:39:07.71 AFV.select :holdingCoil @19:39:07.82 ASV.dial :waste1 @19:39:08.24 ATV.dial :air3 @19:39:09.57 AS.reconfigure ASslow1 @19:39:09.64 AS.seek 0.51ml @19:39:11.94 delay 2 seconds @19:39:13.97 ATV.dial :bleach @19:39:14.62 AS.seek 0.81ml @19:40:15.22 delay 3 seconds @19:40:18.25 ATV.dial :air3 @19:40:18.83 delay 3 seconds @19:40:21.92 AS.seek 0.835ml @19:40:27.30 delay 3 seconds @19:40:30.33 ATV.dial :bleach @19:40:30.98 AS.seek 0.82ml @19:40:34.39 delay 3 seconds @19:40:37.42 ARV.dial :waste @19:40:38.05 delay 3 seconds @19:40:41.08 ATV.dial :ARV @19:40:41.98 AS.reconfigure ASconfig @19:40:42.06 AS.seek 0.47ml @19:41:02.51 AFV.select :carrierSolution @19:41:02.65 delay 2 seconds @19:41:04.69 AS.seek 1ml @19:41:35.44 delay 1 second @19:41:36.46 AFV.select :holdingCoil @19:41:36.58 delay 1 second @19:41:37.61 AS.reconfigure ASfast @19:41:37.68 AS.seek 0ml @19:42:05.03 AFV.select :carrierSolution @19:42:05.16 delay 2 seconds @19:42:07.19 AS.reconfigure ASconfig @19:42:07.26 AS.seek 1ml @19:43:05.06 delay 1 second @19:43:06.08 AFV.select :holdingCoil @19:43:06.20 delay 1 second @19:43:07.23 AS.reconfigure ASfast @19:43:07.30 AS.seek 0ml @19:43:34.67 AFV.select :carrierSolution @19:43:34.80 delay 2 seconds @19:43:36.83 AS.reconfigure ASconfig @19:43:36.90 AS.seek 1ml @19:44:34.66 delay 1 second @19:44:35.68 AFV.select :holdingCoil @19:44:35.80 delay 1 second @19:44:36.83 AS.reconfigure ASfast @19:44:36.90 AS.seek 0ml @19:45:04.25 ARV.dial between carrier and air1 @19:45:04.77 ATV.dial :air3 @19:45:06.09 AS.reconfigure ASslow1 @19:45:06.17 AS.seek 0.01ml @19:45:08.58 delay 2 seconds @19:45:10.60 ATV.dial :bleach @19:45:11.27 AS.seek 0.81ml @19:47:52.49 delay 3 seconds @19:47:55.51 ATV.dial :air3 @19:47:56.08 delay 3 seconds @19:47:59.17 AS.seek 0.835ml @19:48:04.48 delay 3 seconds @19:48:07.51 ATV.dial :bleach @19:48:08.17 AS.seek 0.82ml @19:48:11.42 delay 3 seconds @19:48:14.44 ATV.dial PCR @19:48:14.85 AS.reconfigure ASconfig @19:48:14.91 AS.seek :empty @19:49:02.41 PCR.program :bleachProfile @19:49:02.47 PCR program should complete in < 2:45 @19:49:02.48 PCR.run 10:00 @19:51:58.85 PCR.off @19:51:58.89 AFV.select :carrierSolution @19:51:59.01 delay 2 seconds @19:52:01.05 AS.seek 1ml @19:52:58.84 delay 1 second @19:52:59.86 AFV.select :holdingCoil @19:52:59.98 delay 1 second @19:53:01.01 AS.reconfigure ASfast @19:53:01.08 AS.seek 0ml @19:53:28.45 delay 3 seconds @19:53:31.48 AFV.select :carrierSolution @19:53:31.60 delay 2 seconds @19:53:33.63 AS.reconfigure ASconfig @19:53:33.71 AS.seek 1ml @19:54:31.47 delay 1 second @19:54:32.49 AFV.select :holdingCoil @19:54:32.61 delay 1 second @19:54:33.64 AS.reconfigure ASfast @19:54:33.71 AS.seek 0ml @19:55:01.05 delay 3 seconds @19:55:04.08 AFV.select :carrierSolution @19:55:04.20 delay 2 seconds @19:55:06.23 AS.reconfigure ASconfig @19:55:06.31 AS.seek 1ml @19:56:04.08 delay 1 second @19:56:05.10 AFV.select :holdingCoil @19:56:05.22 delay 1 second @19:56:06.25 AS.reconfigure ASfast @19:56:06.32 AS.seek 0ml @19:56:33.67 delay 3 seconds @19:56:36.70 AFV.select :carrierSolution @19:56:36.82 delay 2 seconds @19:56:38.85 AS.reconfigure ASconfig @19:56:38.92 AS.seek 1ml @19:57:36.69 delay 1 second @19:57:37.71 AFV.select :holdingCoil @19:57:37.83 delay 1 second @19:57:38.86 AS.reconfigure ASfast @19:57:38.93 AS.seek 0ml @19:58:06.27 delay 3 seconds @19:58:09.30 AFV.select :carrierSolution @19:58:09.42 delay 2 seconds @19:58:11.45 AS.reconfigure ASconfig @19:58:11.53 AS.seek 1ml @19:59:09.34 delay 1 second @19:59:10.36 AFV.select :holdingCoil @19:59:10.48 delay 1 second @19:59:11.51 AS.reconfigure ASnorm @19:59:11.58 AS.seek 0ml @20:00:02.30 Setting up PCR for ML -- writing /var/log/esp/ML20may0719h300ml.pcr @20:00:02.32 Building PCR Slug from ASV with MM2 mix, PP4 primer @20:00:02.35 Priming with carrier @20:00:02.37 ATV.dial ARV @20:00:03.02 ARV.dial :carrier @20:00:03.38 AS.seek 0.5ml @20:00:28.96 AFV.select :carrierSolution @20:00:29.10 delay 2 seconds @20:00:31.13 AS.reconfigure ASconfig @20:00:31.21 AS.seek 1ml @20:01:00.24 delay 1 second @20:01:01.26 AFV.select :holdingCoil @20:01:01.38 delay 1 second @20:01:02.41 ATV.dial :waste @20:01:04.11 AS.reconfigure ASnorm @20:01:04.18 AS.seek :empty @20:01:54.97 AFV.select :carrierSolution @20:01:55.10 delay 2 seconds @20:01:57.13 AS.reconfigure ASconfig @20:01:57.20 AS.seek 0.5ml @20:02:26.30 delay 1 second @20:02:27.32 AFV.select :holdingCoil @20:02:27.44 Building Slug @20:02:27.45 ATV.dial :ARV @20:02:29.13 delay 2 seconds @20:02:31.16 ARV.dial :air1 @20:02:31.68 delay 2 seconds @20:02:33.76 AS.reconfigure ASslow2 @20:02:33.84 AS.seek 0.506ml @20:02:40.24 delay 2 seconds @20:02:42.26 ARV.dial :MM2 @20:02:43.78 delay 2 seconds @20:02:45.81 Adding MM2 @20:02:45.91 AS.seek 0.524ml @20:03:04.27 delay 25 seconds @20:03:29.30 ARV.dial :PP4 @20:03:29.72 Adding PP4 @20:03:29.74 delay 2 seconds @20:03:31.83 AS.seek 0.53ml @20:03:38.44 delay 10 seconds @20:03:48.47 ARV.dial :air3 @20:03:48.88 adding ARV air @20:03:48.90 delay 2 seconds @20:03:50.99 AS.seek 0.536ml @20:03:57.30 delay 5 seconds @20:04:02.33 ARV.dial :carrier @20:04:03.63 delay 7 seconds @20:04:10.72 AS.seek 0.594ml @20:05:09.42 delay 5 seconds @20:05:14.45 ATV.dial :ASV @20:05:14.97 delay 2 seconds @20:05:17.64 AS.seek 0.6ml @20:05:23.89 delay 4 seconds @20:05:27.92 ATV.dial ARV @20:05:28.50 delay 2 seconds @20:05:30.53 ARV.dial :carrier @20:05:30.55 delay 2 seconds @20:05:32.65 AS.seek 0.65ml @20:06:23.06 delay 4 seconds @20:06:27.09 ATV.dial PCR @20:06:27.72 ASV.dial :waste1 @20:06:27.74 ARV.dial :air1 @20:06:28.26 Positioning Slug by 0.324ml @20:06:28.37 AS.seek 0.326ml @20:11:54.78 delay 5 seconds @20:11:59.81 ATV.dial ARV @20:12:00.60 ASV.dial :PCRlock @20:12:01.02 delay 5 seconds @20:12:06.05 Running ML Profile60C350 thermocycling @20:12:06.06 PCR.program Profile60C350 @20:12:06.19 Profile60C350 should complete in < 2:02:45 @20:12:06.21 PCR.run 2:38:26.25 @21:49:24.37 PCR.off @21:49:25.67 PCR for ML completed -- closed /var/log/esp/ML20may0719h300ml.pcr @21:49:25.75 Cleaning PCR @21:49:25.77 AFV.select :carrierSolution @21:49:25.90 delay 2 seconds @21:49:27.93 AS.reconfigure ASconfig @21:49:28.00 AS.seek 0.5ml @21:49:38.30 delay 1 second @21:49:39.32 AFV.select :holdingCoil @21:49:39.43 ASV.dial :waste1 @21:49:39.84 ATV.dial :air3 @21:49:41.16 AS.reconfigure ASslow1 @21:49:41.23 AS.seek 0.51ml @21:49:43.53 delay 2 seconds @21:49:45.55 ATV.dial :bleach @21:49:46.21 AS.seek 0.81ml @21:50:46.81 delay 3 seconds @21:50:49.84 ATV.dial :air3 @21:50:50.41 delay 3 seconds @21:50:53.50 AS.seek 0.835ml @21:50:58.82 delay 3 seconds @21:51:01.85 ATV.dial :bleach @21:51:02.49 AS.seek 0.82ml @21:51:05.81 delay 3 seconds @21:51:08.83 ARV.dial :waste @21:51:09.44 delay 3 seconds @21:51:12.47 ATV.dial :ARV @21:51:13.37 AS.reconfigure ASconfig @21:51:14.03 AS.seek 0.47ml @21:51:34.46 AFV.select :carrierSolution @21:51:34.60 delay 2 seconds @21:51:36.64 AS.seek 1ml @21:52:07.39 delay 1 second @21:52:08.41 AFV.select :holdingCoil @21:52:08.53 delay 1 second @21:52:09.56 AS.reconfigure ASfast @21:52:09.63 AS.seek 0ml @21:52:37.00 AFV.select :carrierSolution @21:52:37.13 delay 2 seconds @21:52:39.16 AS.reconfigure ASconfig @21:52:39.23 AS.seek 1ml @21:53:37.00 delay 1 second @21:53:38.02 AFV.select :holdingCoil @21:53:38.14 delay 1 second @21:53:39.17 AS.reconfigure ASfast @21:53:39.24 AS.seek 0ml @21:54:06.59 AFV.select :carrierSolution @21:54:06.72 delay 2 seconds @21:54:08.75 AS.reconfigure ASconfig @21:54:08.83 AS.seek 1ml @21:55:06.59 delay 1 second @21:55:07.61 AFV.select :holdingCoil @21:55:07.73 delay 1 second @21:55:08.76 AS.reconfigure ASfast @21:55:08.83 AS.seek 0ml @21:55:36.20 ARV.dial between carrier and air1 @21:55:36.72 ATV.dial :air3 @21:55:38.04 AS.reconfigure ASslow1 @21:55:38.11 AS.seek 0.01ml @21:55:40.44 delay 2 seconds @21:55:42.46 ATV.dial :bleach @21:55:43.12 AS.seek 0.81ml @21:58:24.38 delay 3 seconds @21:58:27.41 ATV.dial :air3 @21:58:27.99 delay 3 seconds @21:58:31.08 AS.seek 0.835ml @21:58:36.48 delay 3 seconds @21:58:39.51 ATV.dial :bleach @21:58:40.16 AS.seek 0.82ml @21:58:43.49 delay 3 seconds @21:58:46.52 ATV.dial PCR @21:58:46.94 AS.reconfigure ASconfig @21:58:47.00 AS.seek :empty @21:59:34.49 PCR.program :bleachProfile @21:59:34.55 PCR program should complete in < 2:45 @21:59:34.57 PCR.run 10:00 @22:02:30.34 PCR.off @22:02:30.38 AFV.select :carrierSolution @22:02:30.50 delay 2 seconds @22:02:32.54 AS.seek 1ml @22:03:30.39 delay 1 second @22:03:31.41 AFV.select :holdingCoil @22:03:31.53 delay 1 second @22:03:32.56 AS.reconfigure ASfast @22:03:32.63 AS.seek 0ml @22:03:59.97 delay 3 seconds @22:04:03.00 AFV.select :carrierSolution @22:04:03.12 delay 2 seconds @22:04:05.15 AS.reconfigure ASconfig @22:04:05.22 AS.seek 1ml @22:05:03.00 delay 1 second @22:05:04.02 AFV.select :holdingCoil @22:05:04.14 delay 1 second @22:05:05.17 AS.reconfigure ASfast @22:05:05.24 AS.seek 0ml @22:05:32.58 delay 3 seconds @22:05:35.61 AFV.select :carrierSolution @22:05:35.73 delay 2 seconds @22:05:37.76 AS.reconfigure ASconfig @22:05:37.84 AS.seek 1ml @22:06:35.61 delay 1 second @22:06:36.63 AFV.select :holdingCoil @22:06:36.75 delay 1 second @22:06:37.78 AS.reconfigure ASfast @22:06:37.85 AS.seek 0ml @22:07:05.24 delay 3 seconds @22:07:08.27 AFV.select :carrierSolution @22:07:08.39 delay 2 seconds @22:07:10.42 AS.reconfigure ASconfig @22:07:10.50 AS.seek 1ml @22:08:08.27 delay 1 second @22:08:09.29 AFV.select :holdingCoil @22:08:09.41 delay 1 second @22:08:10.44 AS.reconfigure ASfast @22:08:10.51 AS.seek 0ml @22:08:37.86 delay 3 seconds @22:08:40.89 AFV.select :carrierSolution @22:08:41.01 delay 2 seconds @22:08:43.04 AS.reconfigure ASconfig @22:08:43.11 AS.seek 1ml @22:09:40.95 delay 1 second @22:09:41.97 AFV.select :holdingCoil @22:09:42.09 delay 1 second @22:09:43.12 AS.reconfigure ASnorm @22:09:43.19 AS.seek 0ml @22:10:33.96 Setting up PCR for NM -- writing /var/log/esp/NM20may0722h300ml.pcr @22:10:33.98 Building PCR Slug from ASV with MM1 mix, PP1 primer @22:10:34.02 Priming with carrier @22:10:34.04 ATV.dial ARV @22:10:34.69 ARV.dial :carrier @22:10:35.66 AS.seek 0.5ml @22:11:01.21 AFV.select :carrierSolution @22:11:01.34 delay 2 seconds @22:11:03.37 AS.reconfigure ASconfig @22:11:03.44 AS.seek 1ml @22:11:32.50 delay 1 second @22:11:33.52 AFV.select :holdingCoil @22:11:33.64 delay 1 second @22:11:34.67 ATV.dial :waste @22:11:36.36 AS.reconfigure ASnorm @22:11:36.44 AS.seek :empty @22:12:27.24 AFV.select :carrierSolution @22:12:27.37 delay 2 seconds @22:12:29.40 AS.reconfigure ASconfig @22:12:29.47 AS.seek 0.5ml @22:12:58.56 delay 1 second @22:12:59.58 AFV.select :holdingCoil @22:12:59.70 Building Slug @22:12:59.71 ATV.dial :ARV @22:13:01.39 delay 2 seconds @22:13:03.42 ARV.dial :air1 @22:13:03.84 delay 2 seconds @22:13:05.92 AS.reconfigure ASslow2 @22:13:06.00 AS.seek 0.506ml @22:13:12.22 delay 2 seconds @22:13:14.24 ARV.dial :MM1 @22:13:14.86 delay 2 seconds @22:13:16.89 Adding MM1 @22:13:16.98 AS.seek 0.524ml @22:13:35.33 delay 25 seconds @22:14:00.36 ARV.dial :PP1 @22:14:00.78 Adding PP1 @22:14:00.80 delay 2 seconds @22:14:02.89 AS.seek 0.53ml @22:14:09.19 delay 10 seconds @22:14:19.22 ARV.dial :air1 @22:14:19.61 adding ARV air @22:14:19.63 delay 2 seconds @22:14:21.73 AS.seek 0.536ml @22:14:28.20 delay 5 seconds @22:14:33.23 ARV.dial :carrier @22:14:33.66 delay 7 seconds @22:14:40.75 AS.seek 0.594ml @22:15:39.20 delay 5 seconds @22:15:44.23 ATV.dial :ASV @22:15:44.66 delay 2 seconds @22:15:46.75 AS.seek 0.6ml @22:15:52.95 delay 4 seconds @22:15:56.98 ATV.dial ARV @22:15:57.55 delay 2 seconds @22:15:59.58 ARV.dial :carrier @22:15:59.60 delay 2 seconds @22:16:01.69 AS.seek 0.65ml @22:16:52.25 delay 4 seconds @22:16:56.28 ATV.dial PCR @22:16:56.91 ASV.dial :waste1 @22:16:56.93 ARV.dial :air1 @22:16:57.37 Positioning Slug by 0.324ml @22:16:57.48 AS.seek 0.326ml @22:22:23.77 delay 5 seconds @22:22:28.80 ATV.dial ARV @22:22:29.66 ASV.dial :PCRlock @22:22:30.08 delay 5 seconds @22:22:35.11 Running NM Profile60C300 thermocycling @22:22:35.12 PCR.program Profile60C300 @22:22:35.25 Profile60C300 should complete in < 1:59:25 @22:22:35.26 PCR.run 2:34:16.25 @23:58:58.03 PCR.off @23:58:58.72 PCR for NM completed -- closed /var/log/esp/NM20may0722h300ml.pcr @23:58:58.81 Cleaning PCR @23:58:58.82 AFV.select :carrierSolution @23:58:58.96 delay 2 seconds @23:59:00.99 AS.reconfigure ASconfig @23:59:01.06 AS.seek 0.5ml @23:59:11.36 delay 1 second @23:59:12.38 AFV.select :holdingCoil @23:59:12.49 ASV.dial :waste1 @23:59:12.91 ATV.dial :air3 @23:59:14.24 AS.reconfigure ASslow1 @23:59:14.31 AS.seek 0.51ml @23:59:16.61 delay 2 seconds @23:59:18.63 ATV.dial :bleach @23:59:19.29 AS.seek 0.81ml @00:00:19.91CEST08-May-20 delay 3 seconds @00:00:22.94 ATV.dial :air3 @00:00:23.52 delay 3 seconds @00:00:26.61 AS.seek 0.835ml @00:00:31.90 delay 3 seconds @00:00:34.93 ATV.dial :bleach @00:00:35.59 AS.seek 0.82ml @00:00:39.00 delay 3 seconds @00:00:42.03 ARV.dial :waste @00:00:42.66 delay 3 seconds @00:00:45.69 ATV.dial :ARV @00:00:46.59 AS.reconfigure ASconfig @00:00:46.66 AS.seek 0.47ml @00:01:07.10 AFV.select :carrierSolution @00:01:07.24 delay 2 seconds @00:01:09.28 AS.seek 1ml @00:01:40.08 delay 1 second @00:01:41.10 AFV.select :holdingCoil @00:01:41.22 delay 1 second @00:01:42.25 AS.reconfigure ASfast @00:01:42.32 AS.seek 0ml @00:02:09.70 AFV.select :carrierSolution @00:02:09.83 delay 2 seconds @00:02:11.86 AS.reconfigure ASconfig @00:02:11.93 AS.seek 1ml @00:03:09.69 delay 1 second @00:03:10.71 AFV.select :holdingCoil @00:03:10.83 delay 1 second @00:03:11.86 AS.reconfigure ASfast @00:03:11.93 AS.seek 0ml @00:03:39.27 AFV.select :carrierSolution @00:03:39.41 delay 2 seconds @00:03:41.44 AS.reconfigure ASconfig @00:03:41.51 AS.seek 1ml @00:04:39.28 delay 1 second @00:04:40.30 AFV.select :holdingCoil @00:04:40.42 delay 1 second @00:04:41.45 AS.reconfigure ASfast @00:04:41.52 AS.seek 0ml @00:05:08.89 ARV.dial between carrier and air1 @00:05:09.52 ATV.dial :air3 @00:05:10.85 AS.reconfigure ASslow1 @00:05:10.92 AS.seek 0.01ml @00:05:13.33 delay 2 seconds @00:05:15.35 ATV.dial :bleach @00:05:16.01 AS.seek 0.81ml @00:07:57.19 delay 3 seconds @00:08:00.22 ATV.dial :air3 @00:08:00.78 delay 3 seconds @00:08:03.87 AS.seek 0.835ml @00:08:09.19 delay 3 seconds @00:08:12.21 ATV.dial :bleach @00:08:12.85 AS.seek 0.82ml @00:08:16.19 delay 3 seconds @00:08:19.21 ATV.dial PCR @00:08:20.63 AS.reconfigure ASconfig @00:08:20.69 AS.seek :empty @00:09:08.19 PCR.program :bleachProfile @00:09:08.25 PCR program should complete in < 2:45 @00:09:08.27 PCR.run 10:00 @00:12:04.05 PCR.off @00:12:04.09 AFV.select :carrierSolution @00:12:04.20 delay 2 seconds @00:12:06.24 AS.seek 1ml @00:13:04.70 delay 1 second @00:13:05.72 AFV.select :holdingCoil @00:13:05.84 delay 1 second @00:13:06.87 AS.reconfigure ASfast @00:13:06.94 AS.seek 0ml @00:13:34.31 delay 3 seconds @00:13:37.34 AFV.select :carrierSolution @00:13:37.46 delay 2 seconds @00:13:39.49 AS.reconfigure ASconfig @00:13:39.57 AS.seek 1ml @00:14:37.33 delay 1 second @00:14:38.35 AFV.select :holdingCoil @00:14:38.47 delay 1 second @00:14:39.50 AS.reconfigure ASfast @00:14:39.57 AS.seek 0ml @00:15:06.94 delay 3 seconds @00:15:09.97 AFV.select :carrierSolution @00:15:10.09 delay 2 seconds @00:15:12.12 AS.reconfigure ASconfig @00:15:12.19 AS.seek 1ml @00:16:10.02 delay 1 second @00:16:11.04 AFV.select :holdingCoil @00:16:11.16 delay 1 second @00:16:12.19 AS.reconfigure ASfast @00:16:12.26 AS.seek 0ml @00:16:39.63 delay 3 seconds @00:16:42.66 AFV.select :carrierSolution @00:16:42.78 delay 2 seconds @00:16:44.81 AS.reconfigure ASconfig @00:16:44.89 AS.seek 1ml @00:17:42.66 delay 1 second @00:17:43.68 AFV.select :holdingCoil @00:17:43.80 delay 1 second @00:17:44.83 AS.reconfigure ASfast @00:17:44.90 AS.seek 0ml @00:18:12.25 delay 3 seconds @00:18:15.28 AFV.select :carrierSolution @00:18:15.40 delay 2 seconds @00:18:17.43 AS.reconfigure ASconfig @00:18:17.51 AS.seek 1ml @00:19:15.33 delay 1 second @00:19:16.35 AFV.select :holdingCoil @00:19:16.46 delay 1 second @00:19:17.49 AS.reconfigure ASnorm @00:19:17.56 AS.seek 0ml @00:20:08.32 Setting up PCR for IPc -- writing /var/log/esp/IPc20may0800h300ml.pcr @00:20:08.34 Building PCR Slug from ASV with MM3 mix, PP6 primer @00:20:08.37 Priming with carrier @00:20:08.39 ATV.dial ARV @00:20:09.13 ARV.dial :carrier @00:20:09.49 AS.seek 0.5ml @00:20:35.02 AFV.select :carrierSolution @00:20:35.16 delay 2 seconds @00:20:37.19 AS.reconfigure ASconfig @00:20:37.26 AS.seek 1ml @00:21:06.36 delay 1 second @00:21:07.38 AFV.select :holdingCoil @00:21:07.50 delay 1 second @00:21:08.53 ATV.dial :waste @00:21:10.22 AS.reconfigure ASnorm @00:21:10.30 AS.seek :empty @00:22:01.06 AFV.select :carrierSolution @00:22:01.19 delay 2 seconds @00:22:03.22 AS.reconfigure ASconfig @00:22:03.29 AS.seek 0.5ml @00:22:32.41 delay 1 second @00:22:33.43 AFV.select :holdingCoil @00:22:33.55 Building Slug @00:22:33.56 ATV.dial :ARV @00:22:35.24 delay 2 seconds @00:22:37.27 ARV.dial :air1 @00:22:37.70 delay 2 seconds @00:22:39.78 AS.reconfigure ASslow2 @00:22:39.86 AS.seek 0.506ml @00:22:46.06 delay 2 seconds @00:22:48.08 ARV.dial :MM3 @00:22:49.16 delay 2 seconds @00:22:51.19 Adding MM3 @00:22:51.29 AS.seek 0.524ml @00:23:09.56 delay 25 seconds @00:23:34.59 ARV.dial :PP6 @00:23:35.00 Adding PP6 @00:23:35.02 delay 2 seconds @00:23:37.12 AS.seek 0.53ml @00:23:43.41 delay 10 seconds @00:23:53.44 ARV.dial :air1 @00:23:54.38 adding ARV air @00:23:54.40 delay 2 seconds @00:23:56.50 AS.seek 0.536ml @00:24:02.69 delay 5 seconds @00:24:07.72 ARV.dial :carrier @00:24:08.13 delay 7 seconds @00:24:15.22 AS.seek 0.594ml @00:25:13.77 delay 5 seconds @00:25:18.80 ATV.dial :ASV @00:25:19.22 delay 2 seconds @00:25:21.31 AS.seek 0.6ml @00:25:27.72 delay 4 seconds @00:25:31.75 ATV.dial ARV @00:25:32.31 delay 2 seconds @00:25:34.34 ARV.dial :carrier @00:25:34.35 delay 2 seconds @00:25:36.45 AS.seek 0.65ml @00:26:27.03 delay 4 seconds @00:26:31.06 ATV.dial PCR @00:26:31.67 ASV.dial :waste1 @00:26:31.69 ARV.dial :air1 @00:26:32.80 Positioning Slug by 0.324ml @00:26:32.91 AS.seek 0.326ml @00:31:59.00 delay 5 seconds @00:32:04.03 ATV.dial ARV @00:32:04.83 ASV.dial :PCRlock @00:32:05.25 delay 5 seconds @00:32:10.28 Running IPc ProfileIPc thermocycling @00:32:10.29 PCR.program ProfileIPc @00:32:10.43 ProfileIPc should complete in < 1:36:15 @00:32:10.45 PCR.run 2:05:18.75 @01:51:12.15 PCR.off @01:51:12.71 PCR for IPc completed -- closed /var/log/esp/IPc20may0800h300ml.pcr @01:51:12.80 Cleaning PCR @01:51:12.81 AFV.select :carrierSolution @01:51:12.95 delay 2 seconds @01:51:14.98 AS.reconfigure ASconfig @01:51:15.05 AS.seek 0.5ml @01:51:25.41 delay 1 second @01:51:26.43 AFV.select :holdingCoil @01:51:26.54 ASV.dial :waste1 @01:51:26.96 ATV.dial :air3 @01:51:28.27 AS.reconfigure ASslow1 @01:51:28.35 AS.seek 0.51ml @01:51:30.64 delay 2 seconds @01:51:32.66 ATV.dial :bleach @01:51:33.31 AS.seek 0.81ml @01:52:33.92 delay 3 seconds @01:52:36.95 ATV.dial :air3 @01:52:37.50 delay 3 seconds @01:52:40.59 AS.seek 0.835ml @01:52:45.93 delay 3 seconds @01:52:48.95 ATV.dial :bleach @01:52:49.59 AS.seek 0.82ml @01:52:52.89 delay 3 seconds @01:52:55.92 ARV.dial :waste @01:52:56.53 delay 3 seconds @01:52:59.56 ATV.dial :ARV @01:53:00.47 AS.reconfigure ASconfig @01:53:00.54 AS.seek 0.47ml @01:53:20.95 AFV.select :carrierSolution @01:53:21.68 delay 2 seconds @01:53:23.72 AS.seek 1ml @01:53:54.46 delay 1 second @01:53:55.48 AFV.select :holdingCoil @01:53:55.60 delay 1 second @01:53:56.63 AS.reconfigure ASfast @01:53:56.70 AS.seek 0ml @01:54:24.03 AFV.select :carrierSolution @01:54:24.16 delay 2 seconds @01:54:26.19 AS.reconfigure ASconfig @01:54:26.26 AS.seek 1ml @01:55:24.11 delay 1 second @01:55:25.13 AFV.select :holdingCoil @01:55:25.25 delay 1 second @01:55:26.28 AS.reconfigure ASfast @01:55:26.35 AS.seek 0ml @01:55:53.71 AFV.select :carrierSolution @01:55:53.85 delay 2 seconds @01:55:55.88 AS.reconfigure ASconfig @01:55:55.95 AS.seek 1ml @01:56:53.71 delay 1 second @01:56:54.73 AFV.select :holdingCoil @01:56:54.85 delay 1 second @01:56:55.88 AS.reconfigure ASfast @01:56:55.95 AS.seek 0ml @01:57:23.30 ARV.dial between carrier and air1 @01:57:23.82 ATV.dial :air3 @01:57:25.14 AS.reconfigure ASslow1 @01:57:25.21 AS.seek 0.01ml @01:57:27.60 delay 2 seconds @01:57:29.62 ATV.dial :bleach @01:57:30.28 AS.seek 0.81ml @02:00:11.50 delay 3 seconds @02:00:14.53 ATV.dial :air3 @02:00:15.11 delay 3 seconds @02:00:18.20 AS.seek 0.835ml @02:00:23.54 delay 3 seconds @02:00:26.57 ATV.dial :bleach @02:00:27.85 AS.seek 0.82ml @02:00:31.13 delay 3 seconds @02:00:34.16 ATV.dial PCR @02:00:34.57 AS.reconfigure ASconfig @02:00:34.64 AS.seek :empty @02:01:22.10 PCR.program :bleachProfile @02:01:22.16 PCR program should complete in < 2:45 @02:01:22.17 PCR.run 10:00 @02:04:17.96 PCR.off @02:04:18.00 AFV.select :carrierSolution @02:04:18.12 delay 2 seconds @02:04:20.16 AS.seek 1ml @02:05:18.03 delay 1 second @02:05:19.05 AFV.select :holdingCoil @02:05:19.17 delay 1 second @02:05:20.20 AS.reconfigure ASfast @02:05:20.27 AS.seek 0ml @02:05:47.61 delay 3 seconds @02:05:50.64 AFV.select :carrierSolution @02:05:50.76 delay 2 seconds @02:05:52.79 AS.reconfigure ASconfig @02:05:52.87 AS.seek 1ml @02:06:50.63 delay 1 second @02:06:51.65 AFV.select :holdingCoil @02:06:51.77 delay 1 second @02:06:52.80 AS.reconfigure ASfast @02:06:52.87 AS.seek 0ml @02:07:20.24 delay 3 seconds @02:07:23.27 AFV.select :carrierSolution @02:07:23.39 delay 2 seconds @02:07:25.42 AS.reconfigure ASconfig @02:07:25.50 AS.seek 1ml @02:08:23.28 delay 1 second @02:08:24.30 AFV.select :holdingCoil @02:08:24.42 delay 1 second @02:08:25.45 AS.reconfigure ASfast @02:08:25.52 AS.seek 0ml @02:08:52.88 delay 3 seconds @02:08:55.91 AFV.select :carrierSolution @02:08:56.03 delay 2 seconds @02:08:58.06 AS.reconfigure ASconfig @02:08:58.13 AS.seek 1ml @02:09:55.96 delay 1 second @02:09:56.98 AFV.select :holdingCoil @02:09:57.09 delay 1 second @02:09:58.12 AS.reconfigure ASfast @02:09:58.19 AS.seek 0ml @02:10:25.55 delay 3 seconds @02:10:28.58 AFV.select :carrierSolution @02:10:28.70 delay 2 seconds @02:10:30.73 AS.reconfigure ASconfig @02:10:30.81 AS.seek 1ml @02:11:28.58 delay 1 second @02:11:29.60 AFV.select :holdingCoil @02:11:29.71 delay 1 second @02:11:30.74 AS.reconfigure ASnorm @02:11:30.81 AS.seek 0ml @02:12:21.57 Setting up PCR for IPc2 -- writing /var/log/esp/IPc220may0802h300ml.pcr @02:12:21.59 Building PCR Slug from ASV with MM2 mix, PP3 primer @02:12:21.62 Priming with carrier @02:12:21.64 ATV.dial ARV @02:12:22.28 ARV.dial :carrier @02:12:22.64 AS.seek 0.5ml @02:12:48.86 AFV.select :carrierSolution @02:12:48.99 delay 2 seconds @02:12:51.02 AS.reconfigure ASconfig @02:12:51.09 AS.seek 1ml @02:13:20.13 delay 1 second @02:13:21.15 AFV.select :holdingCoil @02:13:21.27 delay 1 second @02:13:22.30 ATV.dial :waste @02:13:24.00 AS.reconfigure ASnorm @02:13:24.08 AS.seek :empty @02:14:14.88 AFV.select :carrierSolution @02:14:15.01 delay 2 seconds @02:14:17.04 AS.reconfigure ASconfig @02:14:17.11 AS.seek 0.5ml @02:14:46.22 delay 1 second @02:14:47.24 AFV.select :holdingCoil @02:14:47.36 Building Slug @02:14:47.37 ATV.dial :ARV @02:14:49.05 delay 2 seconds @02:14:51.08 ARV.dial :air1 @02:14:51.48 delay 2 seconds @02:14:53.57 AS.reconfigure ASslow2 @02:14:53.64 AS.seek 0.506ml @02:14:59.85 delay 2 seconds @02:15:01.87 ARV.dial :MM2 @02:15:03.39 delay 2 seconds @02:15:05.42 Adding MM2 @02:15:05.51 AS.seek 0.524ml @02:15:24.00 delay 25 seconds @02:15:49.03 ARV.dial :PP3 @02:15:49.44 Adding PP3 @02:15:49.46 delay 2 seconds @02:15:51.55 AS.seek 0.53ml @02:15:57.89 delay 10 seconds @02:16:07.92 ARV.dial :air2 @02:16:08.33 adding ARV air @02:16:08.35 delay 2 seconds @02:16:10.44 AS.seek 0.536ml @02:16:16.71 delay 5 seconds @02:16:21.74 ARV.dial :carrier @02:16:23.03 delay 7 seconds @02:16:30.12 AS.seek 0.594ml @02:17:28.80 delay 5 seconds @02:17:33.83 ATV.dial :ASV @02:17:34.25 delay 2 seconds @02:17:36.34 AS.seek 0.6ml @02:17:42.61 delay 4 seconds @02:17:46.64 ATV.dial ARV @02:17:47.21 delay 2 seconds @02:17:49.24 ARV.dial :carrier @02:17:49.26 delay 2 seconds @02:17:51.35 AS.seek 0.65ml @02:18:41.96 delay 4 seconds @02:18:45.99 ATV.dial PCR @02:18:46.63 ASV.dial :waste1 @02:18:46.65 ARV.dial :air1 @02:18:47.08 Positioning Slug by 0.324ml @02:18:47.19 AS.seek 0.326ml @02:24:13.60 delay 5 seconds @02:24:18.63 ATV.dial ARV @02:24:19.42 ASV.dial :PCRlock @02:24:19.85 delay 5 seconds @02:24:24.88 Running IPc2 ProfileIPc thermocycling @02:24:24.90 PCR.program ProfileIPc @02:24:25.02 ProfileIPc should complete in < 1:36:15 @02:24:25.03 PCR.run 2:05:18.75 @03:43:22.43 PCR.off @03:43:23.00 PCR for IPc2 completed -- closed /var/log/esp/IPc220may0802h300ml.pcr @03:43:23.02 Full MFB Decontamination @03:43:23.05 Decon extraction column and decon AIV @03:43:23.09 SPE.hold 2:46:40,55C @03:43:23.14 ASV.dial :SPEdelivery @03:43:23.85 delay 20 seconds @03:43:43.88 AFV.select :carrierSolution @03:43:44.00 delay 2 seconds @03:43:46.03 AS.reconfigure ASconfig @03:43:46.10 AS.seek 0.5ml @03:43:56.44 delay 1 second @03:43:57.46 AFV.select :holdingCoil @03:43:57.58 ATV.dial :air1 @03:43:59.62 AS.reconfigure ASslow1 @03:43:59.69 AS.seek 0.52ml @03:44:04.02 ATV.dial :ASV @03:44:05.93 ASV.dial :SPErecovery @03:44:06.64 AS.seek 0.62ml @03:44:27.05 ATV.dial :waste @03:44:29.18 AS.reconfigure ASnorm @03:44:29.25 AS.seek :empty @03:45:00.91 AFV.select :holdingCoil @03:45:00.92 ATV.dial :waste @03:45:00.96 AS.reconfigure ASconfig @03:45:01.03 AS.seek 0ml @03:45:01.32 AFV.select :carrierSolution @03:45:01.45 delay 2 seconds @03:45:03.49 AS.seek 0.5ml @03:45:32.52 delay 1 second @03:45:33.54 AFV.select :holdingCoil @03:45:33.66 ATV.dial :air3 @03:45:34.43 AS.reconfigure ASslow1 @03:45:34.50 AS.seek 0.52ml @03:45:38.83 ATV.dial :air3 @03:45:38.93 AS.seek 0.53ml @03:45:41.22 delay 2 seconds @03:45:43.24 ATV.dial :bleach @03:45:43.75 AS.seek 0.71ml @03:46:20.22 delay 3 seconds @03:46:23.25 ATV.dial :air3 @03:46:23.86 delay 3 seconds @03:46:26.95 AS.seek 0.735ml @03:46:32.29 delay 3 seconds @03:46:35.31 ATV.dial :bleach @03:46:36.00 AS.seek 0.72ml @03:46:39.32 delay 3 seconds @03:46:42.35 ATV.dial ASV @03:46:43.49 ASV.dial :SPErecovery @03:46:43.59 AS.seek 0.365ml @03:47:55.26 ATV.dial :waste @03:47:57.10 AS.reconfigure ASnorm @03:47:57.17 AS.seek :empty @03:48:15.99 ATV.dial :air3 @03:48:16.73 AS.reconfigure ASslow1 @03:48:16.80 AS.seek 0.009ml @03:48:19.10 delay 2 seconds @03:48:21.12 ATV.dial :bleach @03:48:21.61 AS.seek 0.579ml @03:50:16.54 delay 3 seconds @03:50:19.57 ATV.dial :air3 @03:50:20.16 delay 3 seconds @03:50:23.25 AS.seek 0.604ml @03:50:29.18 delay 3 seconds @03:50:32.21 ATV.dial :bleach @03:50:32.87 AS.seek 0.589ml @03:50:36.32 delay 3 seconds @03:50:39.35 ATV.dial :ASV @03:50:40.41 ASV.dial :wasteR @03:50:40.83 AS.seek 0ml @03:52:39.58 delay 1:00 @03:53:39.61 ATV.dial ASV @03:53:39.62 AFV.select :carrierSolution @03:53:39.75 delay 2 seconds @03:53:41.78 AS.reconfigure ASconfig @03:53:41.85 AS.seek 1ml @03:54:39.63 delay 1 second @03:54:40.65 AFV.select :holdingCoil @03:54:40.77 delay 1 second @03:54:41.80 ASV.dial :wasteR @03:54:41.82 AS.reconfigure ASslow1 @03:54:41.90 AS.seek 0ml @03:58:03.43 delay 10 seconds @03:58:13.46 ATV.dial :air3 @03:58:15.03 AS.seek 0.01ml @03:58:17.33 delay 2 seconds @03:58:19.35 ATV.dial :bleach @03:58:20.00 AS.seek 0.58ml @04:00:14.93 delay 3 seconds @04:00:17.96 ATV.dial :air3 @04:00:18.54 delay 3 seconds @04:00:21.63 AS.seek 0.605ml @04:00:27.00 delay 3 seconds @04:00:30.03 ATV.dial :bleach @04:00:30.67 AS.seek 0.59ml @04:00:34.00 delay 3 seconds @04:00:37.03 ATV.dial ASV @04:00:38.11 ASV.dial :SPEdelivery @04:00:38.51 AS.seek 0ml @04:02:37.44 delay 1:00 @04:03:37.47 AFV.select :carrierSolution @04:03:37.59 delay 2 seconds @04:03:39.62 AS.reconfigure ASconfig @04:03:39.69 AS.seek 1ml @04:04:37.46 delay 1 second @04:04:38.48 AFV.select :holdingCoil @04:04:38.60 delay 1 second @04:04:39.63 ATV.dial ASV @04:04:39.64 ASV.dial :SPEdelivery @04:04:39.68 AS.reconfigure ASslow1 @04:04:39.75 AS.seek 0ml @04:08:01.13 delay 10 seconds @04:08:11.16 ATV.dial :air2 @04:08:12.01 AS.reconfigure ASslow0 @04:08:12.08 AS.seek 0.8ml @04:09:32.85 delay 3 seconds @04:09:35.88 ATV.dial :ASV @04:09:36.91 ASV.dial :SPEdelivery @04:09:36.94 AS.reconfigure ASslow1 @04:09:37.01 AS.seek 0.03ml @04:12:12.16 delay 20 seconds @04:12:32.19 ATV.dial :waste,via: :air3 @04:12:33.96 AS.reconfigure ASconfig @04:12:34.03 AS.seek :empty @04:12:36.12 SPE.coast @04:12:36.23 Removing Bleach from Mixing Coil @04:12:36.24 AFV.select :carrierSolution @04:12:36.36 delay 2 seconds @04:12:38.40 AS.seek 0.5ml @04:13:07.49 delay 1 second @04:13:08.51 AFV.select :holdingCoil @04:13:08.62 ATV.dial :ASV @04:13:10.49 ASV.dial :SPErecovery @04:13:11.18 AS.reconfigure ASslow1 @04:13:11.25 AS.seek 0.73ml @04:13:57.80 ASV.dial :wasteD @04:13:58.49 AS.reconfigure ASnorm @04:13:58.56 AS.seek 0ml @04:14:35.68 AFV.select :carrierSolution @04:14:35.82 delay 2 seconds @04:14:37.85 AS.reconfigure ASconfig @04:14:37.92 AS.seek 0.5ml @04:15:06.96 delay 1 second @04:15:07.98 AFV.select :holdingCoil @04:15:08.10 ATV.dial ASV @04:15:08.12 ASV.dial :SPErecovery @04:15:08.78 AS.reconfigure ASslow0 @04:15:08.85 AS.seek 0.3ml @04:15:29.29 ATV.dial :air2 @04:15:30.22 AS.reconfigure ASslow1 @04:15:30.29 AS.seek 0.32ml @04:15:34.67 ATV.dial ASV @04:15:35.75 AS.reconfigure ASslow0 @04:15:35.82 AS.seek 0.57ml @04:16:01.30 ASV.dial :wasteD @04:16:02.58 AS.reconfigure ASnorm @04:16:02.66 AS.seek 0ml @04:16:31.72 AFV.select :carrierSolution @04:16:31.85 delay 2 seconds @04:16:33.88 AS.reconfigure ASconfig @04:16:33.95 AS.seek 0.5ml @04:17:03.05 delay 1 second @04:17:04.07 AFV.select :holdingCoil @04:17:04.19 ATV.dial ASV @04:17:04.21 ASV.dial :SPErecovery @04:17:04.89 AS.reconfigure ASslow0 @04:17:04.96 AS.seek 0.3ml @04:17:25.43 ATV.dial :air2 @04:17:26.51 AS.reconfigure ASslow1 @04:17:26.58 AS.seek 0.32ml @04:17:30.94 ATV.dial ASV @04:17:32.03 AS.reconfigure ASslow0 @04:17:32.10 AS.seek 0.57ml @04:17:57.58 ASV.dial :wasteD @04:17:58.19 AS.reconfigure ASnorm @04:17:58.27 AS.seek 0ml @04:18:27.35 ATV.dial :air2 @04:18:28.36 AS.reconfigure ASslow0 @04:18:28.44 AS.seek 0.3ml @04:18:59.00 ATV.dial :ASV @04:19:00.00 ASV.dial :wasteD @04:19:00.02 AS.reconfigure ASconfig @04:19:00.10 AS.seek 0.05ml @04:19:14.80 ATV.dial :waste @04:19:16.70 AS.seek 0ml @04:19:19.92 ASV.dial :SPEdelivery @04:19:20.93 deconLysateINT @04:19:20.94 ATV.dial :air3 @04:19:21.67 AS.reconfigure ASslow1 @04:19:21.74 AS.seek 0.01ml @04:19:24.04 delay 2 seconds @04:19:26.06 ATV.dial :bleach @04:19:26.54 AS.seek 0.96ml @04:22:37.93 delay 3 seconds @04:22:40.96 ATV.dial :air3 @04:22:41.54 delay 3 seconds @04:22:44.63 AS.seek 0.985ml @04:22:49.97 delay 3 seconds @04:22:53.00 ATV.dial :bleach @04:22:53.65 AS.seek 0.97ml @04:22:56.96 delay 3 seconds @04:22:59.99 ATV.dial AIV @04:23:01.25 AIV.dial :coilToMFB @04:23:01.27 AWV.select :waste @04:23:01.29 AFV.select :carrierSolution @04:23:01.43 delay 2 seconds @04:23:03.46 AS.reconfigure ASconfig @04:23:03.53 AS.seek 1ml @04:23:05.58 delay 1 second @04:23:06.60 AFV.select :holdingCoil @04:23:06.72 delay 1 second @04:23:07.75 AS.reconfigure ASslow0 @04:23:07.81 AS.seek :empty @04:24:48.76 AFV.select :carrierSolution @04:24:48.90 delay 2 seconds @04:24:50.93 AS.reconfigure ASconfig @04:24:51.00 AS.seek 1ml @04:25:48.85 delay 1 second @04:25:49.87 AFV.select :holdingCoil @04:25:49.99 delay 1 second @04:25:51.02 AS.reconfigure ASslow0 @04:25:51.09 AS.seek :empty @04:27:32.09 AFV.select :carrierSolution @04:27:32.23 delay 2 seconds @04:27:34.26 AS.reconfigure ASconfig @04:27:34.33 AS.seek 1ml @04:28:32.16 delay 1 second @04:28:33.18 AFV.select :holdingCoil @04:28:33.30 delay 1 second @04:28:34.33 AS.reconfigure ASslow0 @04:28:34.40 AS.seek :empty @04:30:15.41 ATV.dial :air2 @04:30:16.12 AS.reconfigure ASslow1 @04:30:16.19 AS.seek 0.009ml @04:30:18.61 AS.reconfigure ASslow0 @04:30:18.68 AS.seek 1ml @04:31:58.65 ATV.dial AIV @04:31:59.48 AS.seek 0.02ml @04:33:38.38 ATV.dial :waste @04:33:40.08 AS.seek 0ml @04:33:42.42 AWV.select :air @04:33:42.61 Cleaning PCR @04:33:42.62 AFV.select :carrierSolution @04:33:42.76 delay 2 seconds @04:33:44.79 AS.reconfigure ASconfig @04:33:44.86 AS.seek 0.5ml @04:34:14.50 delay 1 second @04:34:15.52 AFV.select :holdingCoil @04:34:15.63 ASV.dial :waste1 @04:34:16.04 ATV.dial :air3 @04:34:16.75 AS.reconfigure ASslow1 @04:34:16.82 AS.seek 0.51ml @04:34:19.15 delay 2 seconds @04:34:21.17 ATV.dial :bleach @04:34:21.65 AS.seek 0.81ml @04:35:22.32 delay 3 seconds @04:35:25.35 ATV.dial :air3 @04:35:25.91 delay 3 seconds @04:35:29.00 AS.seek 0.835ml @04:35:34.32 delay 3 seconds @04:35:37.35 ATV.dial :bleach @04:35:37.98 AS.seek 0.82ml @04:35:41.30 delay 3 seconds @04:35:44.33 ARV.dial :waste @04:35:44.96 delay 3 seconds @04:35:47.99 ATV.dial :ARV @04:35:48.90 AS.reconfigure ASconfig @04:35:48.97 AS.seek 0.47ml @04:36:09.40 AFV.select :carrierSolution @04:36:09.53 delay 2 seconds @04:36:11.57 AS.seek 1ml @04:36:42.38 delay 1 second @04:36:43.40 AFV.select :holdingCoil @04:36:43.52 delay 1 second @04:36:44.55 AS.reconfigure ASfast @04:36:44.62 AS.seek 0ml @04:37:12.00 AFV.select :carrierSolution @04:37:12.13 delay 2 seconds @04:37:14.16 AS.reconfigure ASconfig @04:37:14.23 AS.seek 1ml @04:38:12.00 delay 1 second @04:38:13.02 AFV.select :holdingCoil @04:38:13.14 delay 1 second @04:38:14.17 AS.reconfigure ASfast @04:38:14.24 AS.seek 0ml @04:38:41.60 AFV.select :carrierSolution @04:38:41.73 delay 2 seconds @04:38:43.76 AS.reconfigure ASconfig @04:38:43.83 AS.seek 1ml @04:39:41.60 delay 1 second @04:39:42.62 AFV.select :holdingCoil @04:39:42.74 delay 1 second @04:39:43.77 AS.reconfigure ASfast @04:39:43.84 AS.seek 0ml @04:40:11.21 ARV.dial between carrier and air1 @04:40:11.72 ATV.dial :air3 @04:40:13.06 AS.reconfigure ASslow1 @04:40:13.13 AS.seek 0.01ml @04:40:15.43 delay 2 seconds @04:40:17.45 ATV.dial :bleach @04:40:18.11 AS.seek 0.81ml @04:42:59.29 delay 3 seconds @04:43:02.32 ATV.dial :air3 @04:43:02.91 delay 3 seconds @04:43:06.00 AS.seek 0.835ml @04:43:11.30 delay 3 seconds @04:43:14.33 ATV.dial :bleach @04:43:14.98 AS.seek 0.82ml @04:43:18.30 delay 3 seconds @04:43:21.33 ATV.dial PCR @04:43:21.74 AS.reconfigure ASconfig @04:43:21.81 AS.seek :empty @04:44:09.27 PCR.program :bleachProfile @04:44:09.33 PCR program should complete in < 2:45 @04:44:09.35 PCR.run 10:00 @04:47:11.18 PCR.off @04:47:11.22 AFV.select :carrierSolution @04:47:11.34 delay 2 seconds @04:47:13.38 AS.seek 1ml @04:48:11.22 delay 1 second @04:48:12.24 AFV.select :holdingCoil @04:48:12.36 delay 1 second @04:48:13.39 AS.reconfigure ASfast @04:48:13.46 AS.seek 0ml @04:48:40.82 delay 3 seconds @04:48:43.85 AFV.select :carrierSolution @04:48:43.96 delay 2 seconds @04:48:46.00 AS.reconfigure ASconfig @04:48:46.06 AS.seek 1ml @04:49:43.83 delay 1 second @04:49:44.85 AFV.select :holdingCoil @04:49:44.97 delay 1 second @04:49:46.00 AS.reconfigure ASfast @04:49:46.67 AS.seek 0ml @04:50:14.04 delay 3 seconds @04:50:17.07 AFV.select :carrierSolution @04:50:17.19 delay 2 seconds @04:50:19.22 AS.reconfigure ASconfig @04:50:19.30 AS.seek 1ml @04:51:17.08 delay 1 second @04:51:18.10 AFV.select :holdingCoil @04:51:18.22 delay 1 second @04:51:19.25 AS.reconfigure ASfast @04:51:19.32 AS.seek 0ml @04:51:46.68 delay 3 seconds @04:51:49.71 AFV.select :carrierSolution @04:51:49.83 delay 2 seconds @04:51:51.86 AS.reconfigure ASconfig @04:51:51.94 AS.seek 1ml @04:52:49.71 delay 1 second @04:52:50.73 AFV.select :holdingCoil @04:52:50.85 delay 1 second @04:52:51.88 AS.reconfigure ASfast @04:52:51.95 AS.seek 0ml @04:53:19.29 delay 3 seconds @04:53:22.32 AFV.select :carrierSolution @04:53:22.44 delay 2 seconds @04:53:24.47 AS.reconfigure ASconfig @04:53:24.54 AS.seek 1ml @04:54:22.30 delay 1 second @04:54:23.32 AFV.select :holdingCoil @04:54:23.43 delay 1 second @04:54:24.46 AS.reconfigure ASnorm @04:54:24.53 AS.seek 0ml @04:55:15.25 AFV.select :carrierSolution @04:55:15.38 delay 2 seconds @04:55:17.41 AS.reconfigure ASconfig @04:55:17.48 AS.seek 1ml @04:56:15.25 delay 1 second @04:56:16.27 AFV.select :holdingCoil @04:56:16.38 delay 1 second @04:56:17.41 ATV.dial :waste @04:56:18.65 AS.reconfigure ASnorm @04:56:18.73 AS.seek 0.5ml @04:56:44.29 ATV.dial :waste2 @04:56:45.61 AS.seek 0ml @04:57:11.15
Asleep after completing 20invasivedeploymfbtest assays @04:57:11.17 Mission runtime was 15:20:37.656052 @04:57:11.20 GoodNews.email "{1 => 13, 2 => 0}\nCan@04:56:34, 21.1C, 31% humidity, 14.6psia, 11.996V, 0.575A, 0.575A avg, 6.90W",Subject: "Asleep after completing 20invasivedeploymfbtest assays" @04:57:20.51 Session Ended