@1588599712.75CEST = =ECan =tsimfast =hcore =msimfastScheduler =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2018 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi"; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #-> execute '20invasivedeploymfbtest' .GoodNews.suppressMail "Starting 20invasivedeploymfbtest w/pucks in tube 2\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@15:42:01, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "20invasivedeploymfbtest begun" #\Preparing to sleep until +0:00:10 #"shutdown"\Shutdown #t\Asleep til +0:00:10 #\Continuing at 01:00 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: mbaPhase\\n/home/esp/esp2/mission/20invasivedeploymfbtest.rb:119\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@15:42:17, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 01:00" +999 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ P% 9\ % 2\ % *\ S)&\ C' -\ P% 0\ F*\ 9\ C' 2\ .t\SPE.coast F*\ 9\ *\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig F*#,,'T(^20( b S)% h % .t\SS.configure SSconfig C'& S)#.0X 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@15:42:22, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 20invasivedeploymfbtest phase mbaPhase" #\Phase [mbaPhase] #===> STARTED Superphase (phase #1) <===\ Everybody has a plan untill they get punched in the face - Mike Tyson #\Starting mfb #pcrBAC sampling at most 1500ml, wcr at most 1500ml .BACfans.suppressMail "Started mfb in tube 2 at 15:42:22CEST04-May-20\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@15:42:22, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" F*/% #tLoading sh1 puck from tube 2 into Collection Clamp h %&K C'0& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe .tHand.open ."readyMFB"\AFV.select :holdingCoil M&11 F* +6 *2% h %&K ."readyMFB"\ATV.dial :waste2 F*3 % +129 h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *4' +15 h 1 M&5% h %\  A(6% h %X A(7. h ., A(8& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (9& h &  M&:& h &M .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &;( +20 h ( M&<& h & A(=& h &  .t\Forearm.seek 2 + 700 counts M&>' +90 h ' A(?& h &  .t\SE.seek 20.71 Pucks below Top Plate A(@' +56 h ' A(A& h &  M&B& h & .t\Elbow.seek 2 M&C( +7 h ( M&D& h & A(E& h &  .t\Forearm.seek 2 + 300 counts M&F'8 +22 h '8 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &G/ +150 h / ."downElevator"\SE.seek :down M&H& h & A(I'X M&J% h %8 .t\Forearm.seek :retract M&K'\ +57 h 'X ."downElevator"\SE.coast A(5 +34 h '\  .t\Elbow.seek CC M&L(ӷ +143 h (ӷ M&M& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &N& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &O' +91 h ' .t\CC.seek :closed C'P(W +415 h (W .tHand.open M&Q1 +150 h 1 M&R% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &S'\ +91 h '\  .t\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #t\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)T+ +7 h + S)U% h % C'V& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X  +75 h  .t\Exhaust.select :open S)@ +6 .t\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0X% h %4 #tIntake @14.7psia, vacuum from 8.2 to 12.2psi .\SS.reconfigure SSinhale.with maxSpeed: 21 .\SS.seek 25ml S)#.0X% h % S)#% h % .tIntake.select :close S)0 +6 )N% h % .t\SSV.dial :exhaust S)F  +76 h  .t\Exhaust.select :open S)@ +6 .t\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0X  +76 h  .t\Exhaust.select :open S)@ +6 .t\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0X' +511 h ' .tdelay 15 seconds +1500 C'3% h %4 S)N% h % #t\Evacuated: Intake @14.7psia, Outlet @14.7psia .\SSV.dial :spit,avoiding: :exhaust S)F +151 h .t\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0X9 h 9d +100 C'#9 h 96d +100 C'C9 h 9Md +100 C'M9 h 9dd +100 C'Q9 h 9{d +100 C'I9 h 9d +100 C'U9 h 9d +100 C'H9 h 9d +100 C'=9 h 9d +100 C'.9 h 9d +100 C'V9 h 9 d +100 C'$9 h 9 d +100 C'!9 h 9 /d +100 C'Y9 h 9 E d +100 C'X9 h 9 [d +100 C'K9 h 9 qd +100 C'G9 h 9 d +100 C'[9 h 9 d +100 C'T9 h 9  d +100 C'R9 h 9 $d +100 C'\\9 h 9 (d +100 C'_9 h 9 ,d +100 C'Z9 h 9\ 0d +100 C']9 h 9\ !4d +100 C'@9 h 9\ 78d +100 C'd9 h 9\ M ` +19 h ` .t\AIV.dial :bypass C'# { +72 h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'C& h & .tHand.open M&M1 +2 h 1 M&Q& h & M&I% h %\  .t\Elbow.seek FlushPuck::Garage M&U(- +166 h (- M&H& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &=& h &- .t\Forearm.seek :garage M&.'η +91 h 'η .tHand.close M&V/ +150 h / M&$& h &- M&!% h %η .t\Forearm.seek :retract M&Y'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &X(ӷ +264 h (ӷ M&K& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &G& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &[' +91 h ' .t\CC.seek :closed C'T(W +416 h (W C'R& h &W .tHand.open M&\\1 +150 h 1 M&_% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &Z'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%] +151 h P%@% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%d' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%e  +38 h  .t\PS.seek 0ml P%a' +1597 h ' .t\PSV.dial PSR P%^ +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%b' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%c  C'k  h +75 .t\PSV.dial :mixloop P%4 +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%O  +113 h .t\PS.seek 0ml P%h' +2610 h ' .t\PSV.dial :air P%i  +37 h  .t\PS.seek 2.5ml P%l' +2610 h ' .t\PSV.dial :mixloop P%j +38 h .t\PS.seek 0ml P%q' +2609 h ' .t\PSV.dial :air P%m  +38 h  .t\PS.seek 2.5ml P%t' +2610 h ' .t\PSV.dial :mixloop P%` +37 h .t\PS.seek 0ml P%v' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&g& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &W(ӷ +6 h (ӷ M&s& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &w& h &ӷ .t\Forearm.seek CC M&y' +91 h ' .tHand.close M&z/ +150 h / .t\CC.seek :open C'r( +416 h ( M&|& h &ӷ M&u% h % .t\Forearm.seek :retract M&}'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &f(- +265 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &x& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 2 into Collection Clamp C'& h & M&~% h %\  A( % h %X A( . h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (\ & h &  M&o& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +166 h ( M&n& h & A(& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A( & h &  .t\SE.seek 19.71 Pucks below Top Plate A(' +66 h ' A(& h &  M&& h & .t\Elbow.seek 2 M&( +7 h ( M&& h & A(& h &  .t\Forearm.seek 2 + 300 counts M&'8 +22 h '8 .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b & / +150 h / ."downElevator"\SE.seek :down M&{& A('X h & M&(% h %8 .t\Forearm.seek :retract M&*'\ +66 h 'X ."downElevator"\SE.coast A(5 +25 h '\  .t\Elbow.seek CC M&(ӷ +142 h (ӷ M&,& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &%& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'0(W +416 h (W .tHand.open M&21 +150 h 1 M&1% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &L'\ +91 h '\  .t\SC.coast .\CTV.dial CSV,avoiding: :intake A(6 C'S  +151 h  .t\CSV.dial CTV C'P  +18 h  .t\CS.seek 5ml C''p +1373 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'7' +734 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'8'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%<  +38 h  .t\PS.seek 2.5ml P%?' +2609 h ' .t\PSV.dial :mixloop P% +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P% +37 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%D  +151 h  .t\PS.seek :empty P%+' +2610 h ' .t\CBV.dial :waste1 C'& @ +37 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'A  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'/ @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'J  +37 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C': ` +19 h ` .t\CSV.dial :air C' @ +38 h @ .t\CS.seek 10ml C';' +2647 h ' .t\CSV.dial CTV C'B  +38 h  .t\CS.seek 0ml C''4 +2647 h '4 .t\CBV.dial :waste1 C') @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'N  +150 h C''& h &W #t\Storing lyfil puck in Collection Clamp to tube 1 M&>% h %\  A(#% h %X A(C. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (M(( +200 h (( .t\SC.reconfigure SCloose A($.(\ 1~b (Q& h &( M&I& h &ӷ M&6% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &H( +143 h ( M&=& h & A(E& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +89 h ' A("& h &( .t\SE.seek -0.29 Pucks below Top Plate A(9'\\ +265 h '\\ M&V& h & A($& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &!'C +25 h 'C .t\Elbow.hold M&3, +7 h , .t\Elbow.reconfigure TightElbow A(.- h -+\\ M&$&.,(\ #b .t\SE.seek 0.04 Pucks below Top Plate A(K' +26 h ' M&G% h %C A(U& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&Y'o +21 h 'o M&5& h & A(R& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &\\' +22 h ' .t\SE.seek 1.04 Pucks below Top Plate A(_' +36 h ' M&]& h & M&@% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &['\ +89 h '\  .t\Elbow.seek CC M&e(ӷ +143 h (ӷ M&d& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &^& h &ӷ .t\Forearm.seek CC M&a' +91 h ' .tHand.close M&c/ +150 h / .t\CC.seek :open C'k( +416 h ( A(4& h &( A(O& h &( M&b& h &ӷ M&i% h % .t\Forearm.seek :retract M&h'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &j( +142 h ( M&l& h & A(m& h &( .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &q'C +93 h 'C .t\Elbow.hold M&`, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &t1 +150 h 1 M&g% h %C A(s& h &( .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&w'o +20 h 'o M&z& h & A(X& h &( .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &Z' +23 h ' A(T% h % ."downElevator"\SE.seek :down A(|'X +100 (u% h %S M&y% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  +61 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%x  +2 A(6 +111 h  P%W% h % .t\PS.seek 0.499ml P%' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P% +37 h .t\PRV.dial :air P%}  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%  +38 h .t\CBV.dial AIV C'~  +37 h .t\PRV.dial between CBV and air P%  +19 h  .t\PRV.dial :air P%  +19 h  P%\ % h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %o' +250 h ' .t\PRV.dial :waste2 P% @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'n& h & .tHand.open M&1 +2 h 1 M& & h & M&f% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&/ +150 h / M& & h &- M&{% h %η .t\Forearm.seek :retract M&('\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +264 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &,& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &%' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'& h &W .tHand.open M&1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &*'\ +91 h '\  C'r& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%2 @ +76 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%1  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%S  +75 h  .tdelay 2 seconds +200 P%P% h % .t\PS.seek 1.499ml P%' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%' +1094 h ' .t\PSV.dial :air P%F  +38 h  .t\PS.seek 0ml P%' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%7 +37 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%<  +38 h  .tdelay 2 seconds +200 P%v% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%  +75 h .tdelay 2 seconds +200 P%% h %r .t\PS.seek 0.475ml P%D' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%?  +37 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%&'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%A  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%/  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %J' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%: +76 h .t\PTV.dial :PRV,avoiding: :puck P% @ +75 h @ .t\PS.seek 0ml P%8' +2610 h ' .t\PSV.dial :air P%B  +75 h  .t\PS.seek 2.5ml P%;' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%) +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%N' +2609 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%'  +76 h  .t\PS.seek 2.5ml P%>' +2609 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%# +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%C  +75 h P%Q% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %I' +7723 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'6 & +50 h & .t\AWV.select :waste C' +6 P%=% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %E'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'" { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %' +1313 h ' .t\PSV.dial :air P%L  +38 h  .t\PS.seek 2.5ml P%V' +2609 h ' .t\PSV.dial :PML P%$ +38 h .t\PS.seek 0ml P%.' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 'G& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'U& h &W F*5 @@T .t\AWV.select :air ."MFBPCR"\ASV.dial :SPEdelivery .t\AIV.dial :bypass F*R  .t\PSV.dial PSR P%] +22 h  ."MFBPCR"delay 20 seconds +91 h P%@% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +306 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*H I +129 h I F*9% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *!'6 +65 .t\PSV.dial :waste1 P%d  +38 h  .t\PS.seek 0ml P%^' +1597 h ' .t\PSV.dial PSR P%Y +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%K' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%\\ +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%3' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*c  +22 h ' .t\PSV.dial PSR P%[ +21 h  F*0% h %6 ."MFBPCR"\AS.seek 0.329ml F*4'ͯ +92 h P%O% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%b' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*i % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *l'R +1048 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%m  +38 h  .t\PS.seek 0ml P%a' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*e  +22 h  F*t% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *g' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%s +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%z' +217 ."MFBPCR"\ATV.dial :AIV F*X I +43 h I F*T% h % ."MFBPCR"\AS.seek 0.469ml F*h' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%j +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%u' +2609 h ' .t\PSV.dial PSR P%y +113 h P%q% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%w' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%_  +37 h  .t\PS.seek 0ml P%x' +740 h ' ."MFBPCR"delay 5 seconds +500 F*W9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*Z n +21 h n ."MFBPCR"\ASV.dial :SPEdelivery F*|% h % ."MFBPCR"\AS.seek 0.041ml F*M'% +337 h ' .t\PSV.dial PSR P%+ +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%} +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +2610 h ' .t\PSV.dial PSR P%~ +113 h P% % h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P% ' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%\  +38 h  .t\PS.seek 0ml P%' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%o' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *n' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb % 'k +2678 h 'k .t\PSV.dial :waste1 P%f  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F* I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *`'V +850 h ' .t\PSV.dial :air P%  +113 h  .t\PS.seek 2.5ml P% ' +2610 h ' .t\PSV.dial :mixloop P%{ +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +5107 h ' .t\PSV.dial :air P%  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*,  +43 h  F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *('Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*k I +43 h I F*% h %Q ."MFBPCR"\AS.seek 0.01ml F*' +171 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %r' +618 h ' ."MFBPCR"\ATV.dial :waste F*2 n +129 h n ."MFBPCR"\AS.seek :empty F*%'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*1  +86 h  F*S% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *P' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*  +43 h F*% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *F'_ +2670 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'  +38 h  C'7% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'<'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'v' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  '  +38 h  +112  .t\CBV.dial :waste1 C' @ +38 h @ .t\CS.seek 0ml C''4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*?  +21 h ."MFBPCR"delay 3 seconds +300 F*D% h %_ ."MFBPCR"\AS.seek 0.434ml F*A'ݏ +110 h '4 .t\CSV.dial CSR C'/  +38 h  C'&% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C':'P +372 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*  +21 h F*J% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*B'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*8 n +108 h n F*)% h %& ."MFBPCR"\AS.seek 0.092ml F*;'&0 +191 .t\CSR.select :air C' +7 .t\CS.seek 10ml C''' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'N  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'#'4 +2647 h '4 C'C& h &W #tGaraging FlushPuck in Collection Clamp M&Q& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &>(ӷ +6 h (ӷ M&6& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &=& h &ӷ .t\Forearm.seek CC M&I' +91 h ' .tHand.close M&"/ +150 h / .t\CC.seek :open C'E( +416 h ( M&L& h &ӷ M&% h % .t\Forearm.seek :retract M&$'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &V(- +264 h (- M&G& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &U& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &R'η +91 h 'η .tHand.open M&]1 +150 h 1 M&@% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &.'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 2 into Collection Clamp C'H& h & M&9% h %\  A(d% h %X A(p. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (( +201 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (Y& h &  M&^& h &- M&\\% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &K( +166 h ( M&c& h & A(0& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &[' +89 h ' A(O& h &  ."WCR"\SE.seek 18.71 Pucks below Top Plate A(!' +77 h ' A(3& h &  M&i& h & ."WCR"\Elbow.seek 2 M&4( +6 h ( M&m& h & A(e& h &  ."WCR"\Forearm.seek 2 + 300 counts M&t'8 +23 h '8 ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &b/ +150 h / ."downElevator"\SE.seek :down M&l& A(s'X h & M&X% h %8 ."WCR"\Forearm.seek :retract M&T'\ +27 h '&0 ."MFBPCR"delay 3 seconds +49 h 'X ."downElevator"\SE.coast A(5 +15 h '\  ."WCR"\Elbow.seek CC M&a(ӷ +142 h (ӷ M&g& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &j& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &z' +91 h ' ."WCR"\CC.seek :closed C'y(W +3 ."MFBPCR"\ATV.dial :waste2 F*q % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *u'R +370 h (W ."WCR"Hand.open M&_1 +150 h 1 M&w% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &W'\ +40 h 'R ."MFBPCR"\ATV.dial :air2 F*Z  +21 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *|' +30 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)h+ +6 h + S)+% h % C'x& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'}  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X  +75 h  #"WCR"\Allowing 130s for 25.0ml of remaining 1375.0ml .Intake.select :open S) +206 C']% h %4 #"WCR"Intake @14.7psia, vacuum from 8.2 to 12.2psi .\SS.reconfigure SSinhale.with maxSpeed: 21 .\SS.seek 25ml S)#.0X % +43 h % F*]% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*U  +22 h F*H% h % ."MFBPCR"\AS.seek 0.579ml F*9' +4283 h ' #"WCR"\Stroke completed -- waiting for pressure to equalize ... +50 C'd% h %4 S)p% h % S)Y% h % ."WCR"Intake.select :close S)0 +7 )@% h % ."WCR"\SSV.dial :exhaust S)\\  +75 h  ."WCR"\Exhaust.select :open S)@ +6 ."WCR"\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0X% h %4 S)]% h % S)Q% h % ."WCR"Intake.select :close S)0 +6 )8% h % ."WCR"\SSV.dial :exhaust S)U  +76 h  ."WCR"\Exhaust.select :open S)@ +6 ."WCR"\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0X% h %4 S)]% h % S)Q% h % ."WCR"Intake.select :close S)0 +6 )8% h % ."WCR"\SSV.dial :exhaust S)U  +75 h  ."WCR"\Exhaust.select :open S)@ +7 ."WCR"\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0X' +8251 h ' #"WCR"\Stroke completed -- waiting for pressure to equalize ... +50 C']% h %4 S)Q% h % S)8% h % ."WCR"Intake.select :close S)0 +6 )U% h % ."WCR"\SSV.dial :exhaust S)  +75 h  ."WCR"\Exhaust.select :open S)@ +7 ."WCR"\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0X' +800 h ' ."WCR"\Exhaust.select :close S) +6 ."connect"\SSV.dial CBV ."WCR"\CBV.dial SSV S)  +76 h  #"WCR"\Allowing 130s for 25.0ml of remaining 125.0ml .Intake.select :open S) +206 C'Y% h %4 #"WCR"Intake @14.7psia, vacuum from 8.2 to 12.2psi .\SS.reconfigure SSinhale.with maxSpeed: 21 .\SS.seek 25ml S)#.0X& h & M&G% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &L'\ +89 h '\  ."WCR"\Elbow.seek CC M&H(ӷ +143 h (ӷ M&I& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &d& h &ӷ ."WCR"\Forearm.seek CC M&N' +91 h ' ."WCR"Hand.close M&R/ +150 h / ."WCR"\CC.seek :open C'3( +416 h ( A(& h &( A(m& h &( M&e& h &ӷ M&O% h % ."WCR"\Forearm.seek :retract M&^'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &@( +142 h ( M&p& h & A([& h &( ."WCR"\Elbow.reconfigure LimpElbow M&$ ,(#b ."WCR"\Forearm.seek 1 M&b'C +93 h 'C ."WCR"\Elbow.hold M&9, +6 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &\\1 +150 h 1 M&0% h %C A($& h &( ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts +2 M&c'o +20 h 'o M&g& h & A(.& h &( ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &i' +23 h ' A(s% h % ."downElevator"\SE.seek :down A(t'X +100 (;% h %B M&4% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &+'\ +89 h '\  +52 'X ."downElevator"\SE.coast ."WCR"\SC.coast A(5 #t\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'j& h & .tHand.open M&x1 + A(6 + h 1 M&!& h & M&V% h %\  .t\Elbow.seek FlushPuck::Garage M&T(- +166 h (- M&_& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &h& h &- .t\Forearm.seek :garage M&K'η +90 h 'η .tHand.close M&~/ +150 h / M&X& h &- M&\ % h %η .t\Forearm.seek :retract M&E'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &}(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &#& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b & ' +91 h ' .t\CC.seek :closed C'z(W +416 h (W C'u& h &W .tHand.open M&w1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'n& h &W C'% h % .t\CSV.dial CSR C'M  +37 h  C'% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'f'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 ' % h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C', +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +2009 h '4 #t\Flushing lower manifold's waste1 port C' & h &W C'{% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b '('p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  '  +37 h  +113  .t\CBV.dial :waste1 C' @ +76 h @ .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'o& h &W C'P% h %4 .t\CSV.dial :flush C'`  +75 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'k @ +38 h @ C'1% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +37 h  .t\CBV.dial :waste2b C'  +76 h  .t\CS.seek 0ml C'2'4 +766 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'|  +37 h  C'<% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '/' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +37 h  .t\CBV.dial :waste1 C'7 @ +76 h @ .t\CS.seek 0ml C''4 +909 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'J  +38 h  C'S% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '%'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'D' +709 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'v  +38 h  .t\CBV.dial :waste2b C')  +75 h  .t\CS.seek 0ml C'?'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C':  +37 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '"'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'C' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'a 'W  +37 h +76 .t\CTV.dial :air C'  +150 h .t\CS.seek 0ml C'B'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'F'0 +507 h '0 .t\CSV.dial :waste C'Q +37 h .t\CS.seek 0ml C'8'4 +507 h '4 #t\Flushing upper manifold C' & h &W C'*% h %4 .t\CSV.dial :flush C'>  +37 h  .t\CS.seek 5ml C'G'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'& @ +37 h @ C'6% h %p .t\CS.seek 7ml C'''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'= +113 h .t\CTV.dial :air .\CS.seek 0ml C'I'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'd'0 +507 h '0 .t\CSV.dial :waste C'U +37 h .t\CS.seek 0ml C']'4 +507 h '4 C'& h &W C'A% h %4 .t\CSV.dial :flush C'R  +38 h  .t\CS.seek 3ml C'L'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'y @ +38 h @ C'% h %0 .t\CS.seek 5ml C'm'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'e'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'O'0 +507 h '0 .t\CSV.dial :waste C'p +75 h .t\CS.seek 0ml C'['4 +507 h '4 #t\Flushing lower manifold's waste1 port C'N& h &W C'H% h %4 .t\CSV.dial :flush C'\\  +37 h  .t\CS.seek 5ml C'0'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'$ @ +38 h @ C'g% h %p .t\CS.seek 7ml C'.'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C's  '^  +38 h  +113  .t\CBV.dial :waste1 C'@ @ +37 h @ .t\CS.seek 0ml C';'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'4& h &W C'j% h %4 .t\CSV.dial :flush C'x  +76 h  .t\CS.seek 5ml C'!'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'V @ +37 h @ C'b% h %p .t\CS.seek 7ml C'c'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'Y  +38 h  .t\CBV.dial :waste2b C'i  +75 h  .t\CS.seek 0ml C'_'4 +766 h '4 #tGaraging FlushPuck in Collection Clamp M&h& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &t(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &9& h &ӷ .t\Forearm.seek CC M&~' +91 h ' .tHand.close M&X/ +150 h / .t\CC.seek :open C'\ ( +416 h ( M&+& h &ӷ M&K% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &#(- +264 h (- M&T& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &E& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &3'η +91 h 'η .tHand.open M&u1 +150 h 1 M&w% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Completed [mbaPhase] begun 01:00:26 #\Extending 20invasivedeploymfbtest until completion of MFBPCR +408298 !"PCR"o:#AnalyticalModule::PCRRawSample: @refi"; :\ @data""1:1,2,3,4,0.0;2:4,3,2,1,100.0 #"MFBPCR"\PCR for OM completed -- closed /var/log/esp/OM70jan0104h1500ml.pcr F*n% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*M  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*f% h %Z ."MFBPCR"\AS.seek 0.81ml F*,'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*{'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*(  +64 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *o' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *P'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *`' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*k  +32 h  ."MFBPCR"\ATV.dial :air3 F*1 % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*|'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*< % +22 h % ."MFBPCR"delay 3 seconds +300 F*2% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*7% h %m ."MFBPCR"\AS.seek 0.82ml F*/'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*J  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *S'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *%'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *v' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *)'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *D' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *:'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *?'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *"' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *W'V +5060 h 'V #"MFBPCR"\Setting up PCR for CG -- writing /var/log/esp/CG70jan0106h1500ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*a  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*C% h %V ."MFBPCR"\AS.seek 0.5ml F*B'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Q' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*F n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b * 'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b **'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*>  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*8  +21 h ."MFBPCR"delay 2 seconds +200 F*&% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *6'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*G I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*=% h %Fj ."MFBPCR"\AS.seek 0.524ml F*'' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*I n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*U% h % ."MFBPCR"\AS.seek 0.53ml F*d' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*A% h % ."MFBPCR"\AS.seek 0.536ml F*R'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*]  +108 h  ."MFBPCR"delay 7 seconds +700 F*y% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*L n +22 h n ."MFBPCR"delay 2 seconds +200 F*m% h %\  ."MFBPCR"\AS.seek 0.6ml F*e'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*p  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*O% h %\  ."MFBPCR"\AS.seek 0.65ml F*N' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*H  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*\\  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*[% h % \\ ."MFBPCR"\AS.seek 0.326ml F*$'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*g  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*0 % +21 h % ."MFBPCR"delay 5 seconds +500 #Running CG Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:59:25 .\PCR.run 2:34:16.25 +716500 #"MFBPCR"\PCR for CG completed -- closed /var/log/esp/CG70jan0106h1500ml.pcr F*s% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *^'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*@  +22 h ."MFBPCR"\ATV.dial :air3 F*. % +87 h % F*4% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *j'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*x  +22 h F*;% h %Z ."MFBPCR"\AS.seek 0.81ml F*V'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*b % +21 h % ."MFBPCR"delay 3 seconds +300 F*!% h %> ."MFBPCR"\AS.seek 0.835ml F*Y'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*i  +22 h F*c% h %n ."MFBPCR"\AS.seek 0.82ml F*h'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*9  +65 h  F*X% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *}'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *z'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *+' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *K'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *~' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *T'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*E  +31 h  ."MFBPCR"\ATV.dial :air3 F*t % +87 h % F*u% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *w' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*3% h % ."MFBPCR"\AS.seek 0.81ml F*5'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*n % +21 h % ."MFBPCR"delay 3 seconds +300 F*q% h %> ."MFBPCR"\AS.seek 0.835ml F*M'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F* % h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *f'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *,'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *{' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *(' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for ML -- writing /var/log/esp/ML70jan0109h1500ml.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *o' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*P n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *`'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *k'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*1  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +128 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*<' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*2  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*|% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*7 % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*J'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*/  +107 h  ."MFBPCR"delay 7 seconds +700 F*S% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*% n +21 h n ."MFBPCR"delay 2 seconds +200 F*v% h %\  ."MFBPCR"\AS.seek 0.6ml F*)'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*D  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*:% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*?  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*"  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*a% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*C  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*W % +22 h % ."MFBPCR"delay 5 seconds +500 #Running ML Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 2:02:45 .\PCR.run 2:38:26.25 +736500 #"MFBPCR"\PCR for ML completed -- closed /var/log/esp/ML70jan0109h1500ml.pcr F*B% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *F'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*Q  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*>% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *8'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*&  +22 h F**% h %Z ."MFBPCR"\AS.seek 0.81ml F*G'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*= % +22 h % ."MFBPCR"delay 3 seconds +300 F*6% h %> ."MFBPCR"\AS.seek 0.835ml F*I'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*U  +22 h F*'% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*A  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*d  +64 h  F*]% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *y'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*R' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *l'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *L' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *m'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *p'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*O  +32 h  ."MFBPCR"\ATV.dial :air3 F*e % +86 h % F*H% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *\\' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*[  +21 h F*N% h % ."MFBPCR"\AS.seek 0.81ml F*g'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*0 % +22 h % ."MFBPCR"delay 3 seconds +300 F*s% h %> ."MFBPCR"\AS.seek 0.835ml F*$'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*@  +22 h F*.% h %m ."MFBPCR"\AS.seek 0.82ml F*4'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*^  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *x'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*;' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *j'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *!'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *V' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *i'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *c' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Y'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *9'V +5060 h 'V #"MFBPCR"\Setting up PCR for NM -- writing /var/log/esp/NM70jan0111h1500ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*X  +43 h  ."MFBPCR"\ARV.dial :carrier F*h  +11 h  F*}% h %V ."MFBPCR"\AS.seek 0.5ml F* '(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *z' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*+ n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *K'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *~'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*E  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*t  +21 h ."MFBPCR"delay 2 seconds +200 F*u% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *T'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*3% h %Fj ."MFBPCR"\AS.seek 0.524ml F*w' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*n % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*q% h % ."MFBPCR"\AS.seek 0.53ml F*5' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.536ml F*M'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +22 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*f'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +22 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F* '\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*,  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*{  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*(% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running NM Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:59:25 .\PCR.run 2:34:16.25 +716500 #"MFBPCR"\PCR for NM completed -- closed /var/log/esp/NM70jan0111h1500ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*P  +21 h ."MFBPCR"\ATV.dial :air3 F*o % +87 h % F*`% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *1'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*k'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*2  +22 h F*|% h %n ."MFBPCR"\AS.seek 0.82ml F*<'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*7  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b */'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*S' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *J'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *%' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *v'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *D'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*:  +32 h  ."MFBPCR"\ATV.dial :air3 F*) % +86 h % F*?% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *"' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*a  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*C'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*W % +21 h % ."MFBPCR"delay 3 seconds +300 F*B% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*Q  +21 h F* % h %m ."MFBPCR"\AS.seek 0.82ml F*>'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*F  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *&'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F**' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *8'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *=' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *6'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *G' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *U'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *'' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *I'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *A' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *d'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc70jan0114h1500ml.pcr #Building PCR Slug from ASV with MM3 mix, PP6 primer #\Priming with carrier .\ATV.dial ARV F*]  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*y% h %V ."MFBPCR"\AS.seek 0.5ml F*R'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *l' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*L n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *m'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*O  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*e  +22 h ."MFBPCR"delay 2 seconds +200 F*H% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *p'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*[ n +85 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*N% h %Fj ."MFBPCR"\AS.seek 0.524ml F*\\' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP6 F*0  +21 h  #"MFBPCR"\Adding PP6 .delay 2 seconds +200 F*s% h % ."MFBPCR"\AS.seek 0.53ml F*g' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*@  +65 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*.% h % ."MFBPCR"\AS.seek 0.536ml F*$'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*^  +22 h  ."MFBPCR"delay 7 seconds +700 F*4% h %< ."MFBPCR"\AS.seek 0.594ml F*x'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*; n +21 h n ."MFBPCR"delay 2 seconds +200 F*j% h %\  ."MFBPCR"\AS.seek 0.6ml F*b'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*!  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*V% h %\  ."MFBPCR"\AS.seek 0.65ml F*i' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*c  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*Y  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*X'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*h  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*} % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:36:15 .\PCR.run 2:05:18.75 +577500 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc70jan0114h1500ml.pcr F*9% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*+  +22 h ."MFBPCR"\ATV.dial :air3 F*z % +86 h % F*K% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *E'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*t  +22 h F*u% h %Z ."MFBPCR"\AS.seek 0.81ml F*~'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*3% h %> ."MFBPCR"\AS.seek 0.835ml F*T'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*n  +22 h F*q% h %n ."MFBPCR"\AS.seek 0.82ml F*w'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F*5% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b * 'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *M'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *f' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *,'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*{% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*(  +21 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*P  +22 h F*o% h %m ."MFBPCR"\AS.seek 0.82ml F*`'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *1'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *k' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *2'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *|' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *7' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc2 -- writing /var/log/esp/IPc270jan0116h1500ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*<  +11 h  F*/% h %V ."MFBPCR"\AS.seek 0.5ml F*S'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *J' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*% n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *v'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*:  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*)  +21 h ."MFBPCR"delay 2 seconds +200 F*?% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *D'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*a  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*"' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*W  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*B% h % ."MFBPCR"\AS.seek 0.53ml F*C' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*Q  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*F  +108 h  ."MFBPCR"delay 7 seconds +700 F*>% h %< ."MFBPCR"\AS.seek 0.594ml F*&'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F** n +22 h n ."MFBPCR"delay 2 seconds +200 F*8% h %\  ."MFBPCR"\AS.seek 0.6ml F*='\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*6  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*G% h %\  ."MFBPCR"\AS.seek 0.65ml F*U' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*'  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*I  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*A% h % \\ ."MFBPCR"\AS.seek 0.326ml F*]'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*y % +21 h % ."MFBPCR"delay 5 seconds +500 #Running IPc2 ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:36:15 .\PCR.run 2:05:18.75 +577500 #"MFBPCR"\PCR for IPc2 completed -- closed /var/log/esp/IPc270jan0116h1500ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C .\ASV.dial :SPEdelivery F*d @@T *R  +44 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *L'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*l  +151 h F*m% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *O' +423 h ' ."MFBPCR"\ATV.dial :ASV F*e n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*H  +43 h  F*% h % ."MFBPCR"\AS.seek 0.62ml F*['\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*N n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *p'R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *0'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*s'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F*\\ % +43 h % F*@% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *.' +423 h ' ."MFBPCR"\ATV.dial :air3 F*g% h % ."MFBPCR"\AS.seek 0.53ml F*^' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*4  +21 h F*$% h % ."MFBPCR"\AS.seek 0.71ml F*;' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*j % +22 h % ."MFBPCR"delay 3 seconds +300 F*x% h % H ."MFBPCR"\AS.seek 0.735ml F*!' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*V  +22 h F*b% h % Ű ."MFBPCR"\AS.seek 0.72ml F*c' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*Y n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*% h % zG ."MFBPCR"\AS.seek 0.365ml F*i' +7161 h ' ."MFBPCR"\ATV.dial :waste F*h n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *}'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*9 % +43 h % F*X% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *+' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*z  +22 h F*K% h % ."MFBPCR"\AS.seek 0.579ml F* ' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*t % +22 h % ."MFBPCR"delay 3 seconds +300 F*u% h %  ."MFBPCR"\AS.seek 0.604ml F*E'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*3% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*~' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*n n +86 h n ."MFBPCR"\ASV.dial :wasteR F*q  +22 h  ."MFBPCR"\AS.seek 0ml F*T'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b * 'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*5 % +108 h % F*w% h %V ."MFBPCR"\AS.seek 0.01ml F* ' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*M% h % ."MFBPCR"\AS.seek 0.58ml F*' q +11484 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*f% h % q ."MFBPCR"\AS.seek 0.605ml F*'\ 8 +525 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*{% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*,' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*( n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*  +21 h  ."MFBPCR"\AS.seek 0ml F*'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *P' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*o n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F* n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *`'R +202 h 'R ."MFBPCR"\SPE.coast F*  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :ASV F*1 n +150 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *k' +4648 h '  ."MFBPCR"\ASV.dial :wasteD F*2 I +42 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *|'V +3704 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*7  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *<': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*/  +64 h  F*% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *S'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*% n +65 h n F*J% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *v' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*: I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *)'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *?'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  F*a% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*D  +65 h  F*W% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *B'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*" n +65 h n F*Q% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b * ' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*C I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *F'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*>  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +3043 h ': ."MFBPCR"\ATV.dial :ASV F** n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *8'Q +1462 h 'Q ."MFBPCR"\ATV.dial :waste F*& n +151 h n ."MFBPCR"\AS.seek 0ml F*6'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*G  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*= % +43 h % F*'% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *I' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*A  +22 h F*U% h % ."MFBPCR"\AS.seek 0.96ml F*'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*y % +22 h % ."MFBPCR"delay 3 seconds +300 F*R% h %0 ."MFBPCR"\AS.seek 0.985ml F*]' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*l  +22 h F*m% h % ."MFBPCR"\AS.seek 0.97ml F*L'c +323 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*e I +108 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *H' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *O' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *N'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *[' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *p'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*0  +43 h  F*\\% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *@' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *s' +9988 h ' ."MFBPCR"\ATV.dial AIV F*g I +43 h I ."MFBPCR"\AS.seek 0.02ml F*.' +9880 h ' ."MFBPCR"\ATV.dial :waste F*4 n +129 h n ."MFBPCR"\AS.seek 0ml F*$'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*^% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *j'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*x  +22 h ."MFBPCR"\ATV.dial :air3 F*; % +43 h % F*V% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *b'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*!  +22 h F*Y% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*c % +22 h % ."MFBPCR"delay 3 seconds +300 F*h% h %> ."MFBPCR"\AS.seek 0.835ml F*i'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*9  +22 h F*X% h %n ."MFBPCR"\AS.seek 0.82ml F*}'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*z  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*K  +64 h  F*+% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *t'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*u' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *3'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *E' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *n'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*q  +32 h  ."MFBPCR"\ATV.dial :air3 F*~ % +86 h % F*T% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*5  +21 h F*w% h % ."MFBPCR"\AS.seek 0.81ml F* '> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*M% h %> ."MFBPCR"\AS.seek 0.835ml F* 'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*f% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *{'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *('V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *,'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *o' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *P'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*` % +108 h % ."MFBPCR"\AS.seek 0ml F*1'V +2547 h 'V #t\Asleep after completing 20invasivedeploymfbtest assays .GoodNews.suppressMail "{1 => 3, 2 => 19, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@15:44:20, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep after completing 20invasivedeploymfbtest assays" #\Mission runtime was 4:55.549227