@12:49:45.61CET17-Mar-21 <MAIN> ESP2 v3.30 I2C Gateway v3.06 for core bus
@12:49:48.75 
Configuration for mer 7/20/20 brent@mbari.org
/home/esp/esp2/type/shallow/mer/configure.rb
@12:49:48.84 
Configuration for MFB3 by preston@mbari.org
/home/esp/esp2/type/mfb/MFB3/configure.rb
@12:49:55.33 Polling Can every 10:00
@12:49:57.05 CS.configure :default
@12:49:57.09 CS.hold
@12:49:57.20 CS.configure :default
@12:49:57.37 PS.configure :default
@12:49:57.53 SS.configure :default
@12:49:57.61 SS.hold
@12:49:57.70 SS.configure :default
@12:49:57.86 CC.configure :default
@12:49:57.91 CC.hold
@12:49:57.95 CC.configure :default
@12:49:58.03 PC.configure :default
@12:49:58.07 PC.hold
@12:49:58.13 PC.configure :default
@12:49:58.23 Forearm.configure :loose
@12:49:58.27 Forearm.hold
@12:49:58.32 Forearm.configure :loose
@12:49:58.40 Elbow.configure :loose
@12:49:58.44 Elbow.hold
@12:49:58.48 Elbow.configure :loose
@12:49:58.58 SC.configure :loose
@12:49:58.66 SC.hold
@12:49:58.75 SC.configure :loose
@12:49:58.92 SE.configure :default
@12:49:58.99 SE.hold
@12:49:59.08 SE.configure :default
@12:49:59.16 CH.coast
@12:49:59.25 CH.configure :default
@12:49:59.30 PH.coast
@12:49:59.40 PH.configure :default
@12:49:59.50 SPE.coast
@12:49:59.54 SPE.configure SPEconfig
@12:49:59.62 AS.configure ASconfig
@12:49:59.65 AS.hold
@12:49:59.70 AS.configure ASconfig
@12:49:59.75 PCR.off
@12:49:59.78 PCR.pidtemp= 50,0.1,0,300,25,25
@12:49:59.81 PCR.caltemp= 2.5051,0.0372,0
@12:49:59.84 All dwarf controllers running firmware version 3.75
@12:50:00.01 Cleanly exited at 12:49:26CET17-Mar-21
@12:50:00.04 Loading /home/esp/esp2/mission/GENOMAP.rb mission
@12:50:00.32 <MFBPCR> Extracting Nucleic Acid from extSrc
@12:50:00.37 SPE.hold 2:46:40,55C
@12:50:00.45 ASV.dial :SPEdelivery
@12:50:00.83 <bkha> Session Begun
@12:50:00.92 <MFBPCR> delay 20 seconds
@12:50:07.57 <bkha> -> showlog 100
@12:50:20.94 <MFBPCR> AFV.select :holdingCoil
@12:50:21.07 ATV.dial :air2
@12:50:22.51 AS.reconfigure ASslow1
@12:50:22.57 AS.seek 0.07ml
@12:50:36.94 delay 3 seconds
@12:50:39.97 ATV.dial :extSrc
@12:50:41.47 AS.seek 0.47ml
@12:52:02.27 delay 5 seconds
@12:52:07.35 Solid Phase Extraction Heater at 40.3C
@12:52:07.36 ATV.dial ASV
@12:52:08.25 ASV.dial :SPEdelivery
@12:52:08.34 AS.seek 0.042ml
@12:53:34.66 delay 10 seconds
@12:53:44.70 Rinsing external lysate line extSrc
@12:53:44.71 AFV.select :carrierSolution
@12:53:44.82 delay 2 seconds
@12:53:46.84 AS.reconfigure ASconfig
@12:53:46.89 AS.seek 1ml
@12:54:42.25 delay 1 second
@12:54:43.27 AFV.select :holdingCoil
@12:54:43.38 delay 1 second
@12:54:44.40 ATV.dial :extSrc
@12:54:45.23 AS.reconfigure ASnorm
@12:54:45.28 AS.seek 0ml
@12:55:36.00 ATV.dial :air2
@12:55:37.59 AS.reconfigure ASslow0
@12:55:37.65 AS.seek 0.05ml
@12:55:43.03 ATV.dial :extSrc
@12:55:44.61 AS.seek 0.01ml
@12:55:48.96 ATV.dial :waste
@12:55:50.34 AS.reconfigure ASconfig
@12:55:50.39 AS.seek 0ml
@12:55:51.60 ethanol wash
@12:55:51.61 ATV.dial :air2
@12:55:52.80 AS.reconfigure ASslow2
@12:55:52.86 AS.seek 0.01ml
@12:56:03.27 delay 3 seconds
@12:56:06.30 Pulling 0.4ml of Ethanol Wash
@12:56:06.31 ATV.dial :ethanol
@12:56:07.20 AS.reconfigure ASslow1
@12:56:07.26 AS.seek 0.41ml
@12:57:27.99 delay 3 seconds
@12:57:31.02 ATV.dial :air1
@12:57:31.44 delay 3 seconds
@12:57:34.51 AS.seek 0.435ml
@12:57:39.84 delay 3 seconds
@12:57:42.87 ATV.dial :ethanol
@12:57:43.51 AS.seek 0.42ml
@12:57:46.81 delay 3 seconds
@12:57:49.84 ATV.dial ASV
@12:57:51.23 AS.seek 0.093ml
@12:58:57.36 delay 3 seconds
@12:59:00.39 ATV.dial :waste2
@12:59:01.20 AS.reconfigure ASconfig
@12:59:01.25 AS.seek 0ml
@12:59:06.91 ATV.dial :air2
@12:59:07.34 AS.reconfigure ASslow1
@12:59:07.39 AS.seek 1ml
@13:02:28.80 delay 5 seconds
@13:02:33.83 ATV.dial ASV
@13:02:34.88 ASV.dial :SPEdelivery
@13:02:34.91 AS.seek 0.02ml
@13:05:52.25 delay 5 seconds
@13:05:57.28 ATV.dial :air2
@13:05:58.37 AS.seek 0.52ml
@13:07:39.24 delay 5 seconds
@13:07:44.27 ATV.dial ASV
@13:07:45.31 ASV.dial :wasteR
@13:07:45.70 AS.seek 0.3ml
@13:08:30.24 delay 20 seconds
@13:08:50.28 AS.seek 0.07ml
@13:09:36.80 delay 20 seconds
@13:09:56.83 ATV.dial :waste2
@13:09:57.64 AS.reconfigure ASconfig
@13:09:57.69 AS.seek 0ml
@13:10:02.02 Elution
@13:10:02.03 ATV.dial :air1
@13:10:03.23 AS.reconfigure ASslow1
@13:10:03.28 AS.seek 0.05ml
@13:10:13.63 delay 3 seconds
@13:10:16.66 ATV.dial :ElutionWater
@13:10:17.37 AS.seek 0.11ml
@13:10:29.69 delay 3 seconds
@13:10:32.72 ATV.dial ASV,via: :air2
@13:10:34.95 ASV.dial :SPEelution
@13:10:35.56 AFV.select :carrierSolution
@13:10:35.72 AS.reconfigure ASnorm
@13:10:35.78 AS.seek 0.76ml
@13:11:08.83 delay 3 seconds
@13:11:11.85 AFV.select :holdingCoil
@13:11:12.00 AS.reconfigure ASslow1
@13:11:12.06 AS.seek 0.475ml
@13:12:09.69 delay 5 seconds
@13:12:14.77 AS.reconfigure ASslow2
@13:12:14.83 AS.seek 0.44ml
@13:12:50.13 delay 1:00
@13:13:50.17 pushing eluate to holding coil
@13:13:50.24 AS.seek 0.28ml
@13:16:31.53 delay 30 seconds
@13:17:01.57 ATV.dial :waste,via: :air3
@13:17:03.42 AS.reconfigure ASconfig
@13:17:03.47 AS.seek 0ml
@13:17:19.89 Parking Bleach in LysateSource line and SPEcolumn
@13:17:19.90 ATV.dial :air3
@13:17:20.81 AS.reconfigure ASslow1
@13:17:20.86 AS.seek 0.03ml
@13:17:27.19 ATV.dial :ElutionWater
@13:17:27.84 AS.seek 0.06ml
@13:17:34.13 ATV.dial :air3
@13:17:34.78 AS.seek 0.07ml
@13:17:37.05 delay 2 seconds
@13:17:39.08 ATV.dial :bleach
@13:17:39.59 AS.seek 0.15ml
@13:17:56.03 delay 3 seconds
@13:17:59.06 ATV.dial :air3
@13:17:59.66 delay 3 seconds
@13:18:02.73 AS.seek 0.175ml
@13:18:08.06 delay 3 seconds
@13:18:11.09 ATV.dial :bleach
@13:18:11.75 AS.seek 0.16ml
@13:18:15.11 delay 3 seconds
@13:18:18.14 ATV.dial :extSrc
@13:18:18.79 AS.reconfigure ASconfig
@13:18:18.84 AS.seek 0.015ml
@13:18:27.49 ATV.dial :waste
@13:18:29.04 AS.seek 0ml
@13:18:30.17 ATV.dial :air3
@13:18:31.06 AS.reconfigure ASslow1
@13:18:31.12 AS.seek 0.01ml
@13:18:33.45 delay 2 seconds
@13:18:35.48 ATV.dial :bleach
@13:18:35.95 AS.seek 0.58ml
@13:20:30.85 delay 3 seconds
@13:20:33.88 ATV.dial :air3
@13:20:34.45 delay 3 seconds
@13:20:37.52 AS.seek 0.605ml
@13:20:42.86 delay 3 seconds
@13:20:45.89 ATV.dial :bleach
@13:20:46.53 AS.seek 0.59ml
@13:20:49.78 delay 3 seconds
@13:20:52.81 ATV.dial :ASV
@13:20:53.91 ASV.dial :wasteR
@13:20:54.54 AS.seek 0ml
@13:22:53.44 delay 1:00
@13:23:53.47 AFV.select :carrierSolution
@13:23:53.58 delay 2 seconds
@13:23:55.60 AS.reconfigure ASconfig
@13:23:55.65 AS.seek 1ml
@13:24:53.39 delay 1 second
@13:24:54.41 AFV.select :holdingCoil
@13:24:54.52 delay 1 second
@13:24:55.54 ATV.dial ASV
@13:24:55.55 ASV.dial :wasteR
@13:24:55.57 AS.reconfigure ASslow1
@13:24:55.64 AS.seek 0ml
@13:28:17.08 delay 20 seconds
@13:28:37.12 ATV.dial :air3
@13:28:38.72 AS.seek 0.59ml
@13:30:37.64 delay 3 seconds
@13:30:40.67 ATV.dial :ASV
@13:30:42.17 ASV.dial :wasteR
@13:30:42.26 AS.seek 0.09ml
@13:32:23.10 delay 30 seconds
@13:32:53.14 ATV.dial :extSrc
@13:32:53.95 delay 3 seconds
@13:32:56.98 ATV.dial :ElutionWater
@13:32:58.18 AS.seek 0.12ml
@13:33:04.55 ATV.dial :air3
@13:33:05.20 AS.seek 0.13ml
@13:33:07.49 delay 2 seconds
@13:33:09.52 ATV.dial :bleach
@13:33:10.01 AS.seek 0.23ml
@13:33:30.39 delay 3 seconds
@13:33:33.42 ATV.dial :air3
@13:33:34.00 delay 3 seconds
@13:33:37.07 AS.seek 0.255ml
@13:33:42.37 delay 3 seconds
@13:33:45.40 ATV.dial :bleach
@13:33:46.03 AS.seek 0.24ml
@13:33:49.75 delay 3 seconds
@13:33:52.78 ATV.dial :ASV
@13:33:53.89 ASV.dial :SPEdelivery
@13:33:54.28 AFV.select :carrierSolution
@13:33:54.40 delay 2 seconds
@13:33:56.42 AS.reconfigure ASconfig
@13:33:56.47 AS.seek 0.5ml
@13:34:11.70 delay 1 second
@13:34:12.72 AFV.select :holdingCoil
@13:34:12.87 AS.reconfigure ASslow1
@13:34:12.93 AS.seek 0.17ml
@13:35:19.56 SPE.hold 2:46:40,95C
@13:35:19.62 delay 3:00
@13:38:19.66 AFV.select :carrierSolution
@13:38:19.78 delay 2 seconds
@13:38:21.80 AS.reconfigure ASconfig
@13:38:21.85 AS.seek 1ml
@13:39:09.85 delay 1 second
@13:39:10.87 AFV.select :holdingCoil
@13:39:10.98 delay 1 second
@13:39:12.00 ASV.dial :wasteD
@13:39:13.12 AS.seek 0ml
@13:40:10.89 Priming Eluate
@13:40:10.90 AFV.select :carrierSolution
@13:40:11.03 delay 2 seconds
@13:40:13.05 AS.seek 0.5ml
@13:40:42.06 delay 1 second
@13:40:43.08 AFV.select :holdingCoil
@13:40:43.19 ATV.dial ASV
@13:40:43.20 ASV.dial :SPErecovery
@13:40:43.83 Swish #1 of 2
@13:40:43.91 AS.reconfigure ASslow0
@13:40:43.97 AS.seek 0.6ml
@13:40:54.44 AS.seek 0.5ml
@13:41:04.88 Swish #2 of 2
@13:41:04.97 AS.seek 0.6ml
@13:41:15.36 AS.seek 0.5ml
@13:41:25.78 delay 3 seconds
@13:41:28.85 AS.reconfigure ASslow1
@13:41:28.91 AS.seek 0.684ml
@13:42:06.25 delay 3 seconds
@13:42:09.28 Eluate primed
@13:42:09.29 ATV.dial :waste2
@13:42:09.98 AS.reconfigure ASnorm
@13:42:10.04 AS.seek 0ml
@13:42:44.83 SPE.coast
@13:42:44.91 ASV.dial :SPErecovery
@13:42:44.93 Setting up PCR for ACYPP2 -- writing /var/log/esp/ACYPP221mar1713h.pcr
@13:42:44.96 Building PCR Slug from ASV with MM1 mix, PP2 primer
@13:42:44.98 Priming with carrier
@13:42:44.99 ATV.dial ARV
@13:42:46.05 ARV.dial :carrier
@13:42:46.41 AS.seek 0.5ml
@13:43:12.00 AFV.select :carrierSolution
@13:43:12.13 delay 2 seconds
@13:43:14.15 AS.reconfigure ASconfig
@13:43:14.21 AS.seek 1ml
@13:43:43.22 delay 1 second
@13:43:44.24 AFV.select :holdingCoil
@13:43:44.35 delay 1 second
@13:43:45.37 ATV.dial :waste
@13:43:47.00 AS.reconfigure ASnorm
@13:43:47.06 AS.seek 0ml
@13:44:37.77 AFV.select :carrierSolution
@13:44:37.89 delay 2 seconds
@13:44:39.91 AS.reconfigure ASconfig
@13:44:39.97 AS.seek 0.5ml
@13:45:09.00 delay 1 second
@13:45:10.03 AFV.select :holdingCoil
@13:45:10.13 Building Slug
@13:45:10.14 ATV.dial :ARV
@13:45:11.91 delay 2 seconds
@13:45:13.94 ARV.dial :air1
@13:45:14.33 delay 2 seconds
@13:45:16.40 AS.reconfigure ASslow2
@13:45:16.46 AS.seek 0.506ml
@13:45:22.67 delay 2 seconds
@13:45:24.70 ARV.dial :MM1
@13:45:25.33 delay 2 seconds
@13:45:27.36 Adding MM1
@13:45:27.43 AS.seek 0.524ml
@13:45:45.64 delay 25 seconds
@13:46:10.67 ARV.dial :PP2
@13:46:11.07 Adding PP2
@13:46:11.08 delay 2 seconds
@13:46:13.15 AS.seek 0.53ml
@13:46:19.53 delay 10 seconds
@13:46:29.57 ARV.dial :air2
@13:46:30.00 adding ARV air
@13:46:30.01 delay 2 seconds
@13:46:32.09 AS.seek 0.536ml
@13:46:38.74 delay 5 seconds
@13:46:43.77 ARV.dial :carrier
@13:46:45.10 delay 7 seconds
@13:46:52.18 AS.seek 0.594ml
@13:47:51.03 delay 5 seconds
@13:47:56.06 ATV.dial :ASV
@13:47:56.47 delay 2 seconds
@13:47:58.55 AS.seek 0.6ml
@13:48:05.19 delay 4 seconds
@13:48:09.22 ATV.dial ARV
@13:48:09.80 delay 2 seconds
@13:48:11.83 ARV.dial :carrier
@13:48:11.84 delay 2 seconds
@13:48:13.92 AS.seek 0.65ml
@13:49:04.61 delay 4 seconds
@13:49:08.64 ATV.dial PCR
@13:49:09.28 ASV.dial :waste1
@13:49:10.10 ARV.dial :air1
@13:49:10.51 Positioning Slug by 0.324ml
@13:49:10.59 AS.seek 0.326ml
@13:54:36.63 delay 5 seconds
@13:54:41.66 ATV.dial ARV
@13:54:42.47 ASV.dial :PCRlock
@13:54:42.86 delay 5 seconds
@13:54:47.89 Running ACYPP2 Profile60CIQ2 thermocycling
@13:54:47.90 <PCR> PCR.program Profile60CIQ2
@13:54:48.03 Profile60CIQ2 should complete in < 1:47:50
@13:54:48.04 PCR.run 2:19:47.5
@15:23:14.55 PCR.off
@15:23:15.17 <MFBPCR> Running ACYPP2 MeltProfile60 thermocycling
@15:23:15.55 <PCR> PCR.program MeltProfile60
@15:23:15.66 MeltProfile60 should complete in < 11:55
@15:23:15.67 PCR.run 19:53.75
@15:30:50.44 PCR.off
@15:30:51.38 <MFBPCR> PCR for ACYPP2 completed -- closed /var/log/esp/ACYPP221mar1713h.pcr
@15:30:51.45 Cleaning PCR
@15:30:51.46 AFV.select :carrierSolution
@15:30:51.58 delay 2 seconds
@15:30:53.60 AS.reconfigure ASconfig
@15:30:53.65 AS.seek 0.5ml
@15:31:03.94 delay 1 second
@15:31:04.96 AFV.select :holdingCoil
@15:31:05.07 ASV.dial :waste1
@15:31:05.47 ATV.dial :air3
@15:31:06.93 AS.reconfigure ASslow1
@15:31:07.05 AS.seek 0.51ml
@15:31:09.33 delay 2 seconds
@15:31:11.36 ATV.dial :bleach
@15:31:12.00 AS.seek 0.81ml
@15:32:12.56 delay 3 seconds
@15:32:15.59 ATV.dial :air3
@15:32:16.16 delay 3 seconds
@15:32:19.23 AS.seek 0.835ml
@15:32:24.53 delay 3 seconds
@15:32:27.56 ATV.dial :bleach
@15:32:28.20 AS.seek 0.82ml
@15:32:31.52 delay 3 seconds
@15:32:34.55 ARV.dial :waste
@15:32:35.17 delay 3 seconds
@15:32:38.20 ATV.dial :ARV
@15:32:39.12 AS.reconfigure ASconfig
@15:32:39.18 AS.seek 0.47ml
@15:32:59.58 AFV.select :carrierSolution
@15:32:59.70 delay 2 seconds
@15:33:01.72 AS.seek 1ml
@15:33:32.46 delay 1 second
@15:33:33.48 AFV.select :holdingCoil
@15:33:33.59 delay 1 second
@15:33:34.61 AS.reconfigure ASfast
@15:33:34.67 AS.seek 0ml
@15:34:01.99 AFV.select :carrierSolution
@15:34:02.11 delay 2 seconds
@15:34:04.13 AS.reconfigure ASconfig
@15:34:04.18 AS.seek 1ml
@15:35:01.96 delay 1 second
@15:35:02.98 AFV.select :holdingCoil
@15:35:03.09 delay 1 second
@15:35:04.11 AS.reconfigure ASfast
@15:35:04.17 AS.seek 0ml
@15:35:31.63 AFV.select :carrierSolution
@15:35:31.75 delay 2 seconds
@15:35:33.77 AS.reconfigure ASconfig
@15:35:33.82 AS.seek 1ml
@15:36:31.58 delay 1 second
@15:36:32.60 AFV.select :holdingCoil
@15:36:32.71 delay 1 second
@15:36:33.73 AS.reconfigure ASfast
@15:36:33.79 AS.seek 0ml
@15:37:01.11 ARV.dial between carrier and air1
@15:37:01.61 ATV.dial :air3
@15:37:02.97 AS.reconfigure ASslow1
@15:37:03.03 AS.seek 0.01ml
@15:37:05.34 delay 2 seconds
@15:37:07.36 ATV.dial :bleach
@15:37:08.00 AS.seek 0.81ml
@15:39:49.13 delay 3 seconds
@15:39:52.16 ATV.dial :air3
@15:39:52.74 delay 3 seconds
@15:39:55.81 AS.seek 0.835ml
@15:40:01.10 delay 3 seconds
@15:40:04.13 ATV.dial :bleach
@15:40:04.77 AS.seek 0.82ml
@15:40:08.13 delay 3 seconds
@15:40:11.16 ATV.dial PCR
@15:40:11.59 AS.reconfigure ASconfig
@15:40:11.64 AS.seek 0ml
@15:40:59.06 PCR.program :bleachProfile
@15:40:59.13 PCR program should complete in < 2:45
@15:40:59.14 PCR.run 10:00
@15:43:55.34 PCR.off
@15:43:55.38 AFV.select :carrierSolution
@15:43:55.49 delay 2 seconds
@15:43:57.51 AS.seek 1ml
@15:44:55.27 delay 1 second
@15:44:56.29 AFV.select :holdingCoil
@15:44:56.40 delay 1 second
@15:44:57.42 AS.reconfigure ASfast
@15:44:57.48 AS.seek 0ml
@15:45:24.81 delay 3 seconds
@15:45:27.83 AFV.select :carrierSolution
@15:45:27.94 delay 2 seconds
@15:45:29.96 AS.reconfigure ASconfig
@15:45:30.02 AS.seek 1ml
@15:46:27.78 delay 1 second
@15:46:28.80 AFV.select :holdingCoil
@15:46:28.91 delay 1 second
@15:46:29.93 AS.reconfigure ASfast
@15:46:29.99 AS.seek 0ml
@15:46:57.32 delay 3 seconds
@15:47:00.34 AFV.select :carrierSolution
@15:47:00.45 delay 2 seconds
@15:47:02.47 AS.reconfigure ASconfig
@15:47:02.53 AS.seek 1ml
@15:48:00.28 delay 1 second
@15:48:01.30 AFV.select :holdingCoil
@15:48:01.41 delay 1 second
@15:48:02.43 AS.reconfigure ASfast
@15:48:02.49 AS.seek 0ml
@15:48:29.83 delay 3 seconds
@15:48:32.85 AFV.select :carrierSolution
@15:48:32.96 delay 2 seconds
@15:48:34.98 AS.reconfigure ASconfig
@15:48:35.05 AS.seek 1ml
@15:49:32.83 delay 1 second
@15:49:33.85 AFV.select :holdingCoil
@15:49:33.96 delay 1 second
@15:49:34.98 AS.reconfigure ASfast
@15:49:35.04 AS.seek 0ml
@15:50:02.38 delay 3 seconds
@15:50:05.40 AFV.select :carrierSolution
@15:50:05.51 delay 2 seconds
@15:50:07.53 AS.reconfigure ASconfig
@15:50:07.59 AS.seek 1ml
@15:51:05.38 delay 1 second
@15:51:06.40 AFV.select :holdingCoil
@15:51:06.51 delay 1 second
@15:51:07.53 AS.reconfigure ASnorm
@15:51:07.58 AS.seek 0ml
@15:51:58.30 Setting up PCR for AOPP1 -- writing /var/log/esp/AOPP121mar1715h.pcr
@15:51:58.31 Building PCR Slug from ASV with MM1 mix, PP1 primer
@15:51:58.34 Priming with carrier
@15:51:58.35 ATV.dial ARV
@15:51:59.02 ARV.dial :carrier
@15:51:59.38 AS.seek 0.5ml
@15:52:24.96 AFV.select :carrierSolution
@15:52:25.08 delay 2 seconds
@15:52:27.10 AS.reconfigure ASconfig
@15:52:27.17 AS.seek 1ml
@15:52:56.19 delay 1 second
@15:52:57.21 AFV.select :holdingCoil
@15:52:57.32 delay 1 second
@15:52:58.34 ATV.dial :waste
@15:53:00.12 AS.reconfigure ASnorm
@15:53:00.18 AS.seek 0ml
@15:53:50.85 AFV.select :carrierSolution
@15:53:50.97 delay 2 seconds
@15:53:52.99 AS.reconfigure ASconfig
@15:53:53.05 AS.seek 0.5ml
@15:54:22.11 delay 1 second
@15:54:23.13 AFV.select :holdingCoil
@15:54:23.24 Building Slug
@15:54:23.25 ATV.dial :ARV
@15:54:25.00 delay 2 seconds
@15:54:27.03 ARV.dial :air1
@15:54:27.43 delay 2 seconds
@15:54:29.50 AS.reconfigure ASslow2
@15:54:29.56 AS.seek 0.506ml
@15:54:35.89 delay 2 seconds
@15:54:37.92 ARV.dial :MM1
@15:54:38.55 delay 2 seconds
@15:54:40.58 Adding MM1
@15:54:40.65 AS.seek 0.524ml
@15:54:59.17 delay 25 seconds
@15:55:24.20 ARV.dial :PP1
@15:55:24.62 Adding PP1
@15:55:24.63 delay 2 seconds
@15:55:26.70 AS.seek 0.53ml
@15:55:33.07 delay 10 seconds
@15:55:43.11 ARV.dial :air1
@15:55:43.51 adding ARV air
@15:55:43.52 delay 2 seconds
@15:55:45.59 AS.seek 0.536ml
@15:55:51.97 delay 5 seconds
@15:55:57.00 ARV.dial :carrier
@15:55:57.42 delay 7 seconds
@15:56:04.51 AS.seek 0.594ml
@15:57:02.99 delay 5 seconds
@15:57:08.02 ATV.dial :ASV
@15:57:08.44 delay 2 seconds
@15:57:10.52 AS.seek 0.6ml
@15:57:16.71 delay 4 seconds
@15:57:20.74 ATV.dial ARV
@15:57:21.32 delay 2 seconds
@15:57:23.35 ARV.dial :carrier
@15:57:23.36 delay 2 seconds
@15:57:25.44 AS.seek 0.65ml
@15:58:16.08 delay 4 seconds
@15:58:20.11 ATV.dial PCR
@15:58:20.75 ASV.dial :waste1
@15:58:20.77 ARV.dial :air1
@15:58:21.19 Positioning Slug by 0.324ml
@15:58:21.27 AS.seek 0.326ml
@16:03:47.42 delay 5 seconds
@16:03:52.45 ATV.dial ARV
@16:03:53.25 ASV.dial :PCRlock
@16:03:54.08 delay 5 seconds
@16:03:59.11 Running AOPP1 Profile60CIQ1 thermocycling
@16:03:59.12 <PCR> PCR.program Profile60CIQ1
@16:03:59.24 Profile60CIQ1 should complete in < 1:56:15
@16:03:59.25 PCR.run 2:30:18.75
@17:42:57.25 PCR.off
@17:42:57.81 <MFBPCR> Running AOPP1 MeltProfile60 thermocycling
@17:42:57.83 <PCR> PCR.program MeltProfile60
@17:42:57.92 MeltProfile60 should complete in < 11:55
@17:42:57.93 PCR.run 19:53.75
@17:50:32.81 PCR.off
@17:50:33.74 <MFBPCR> PCR for AOPP1 completed -- closed /var/log/esp/AOPP121mar1715h.pcr
@17:50:33.81 Cleaning PCR
@17:50:33.82 AFV.select :carrierSolution
@17:50:33.95 delay 2 seconds
@17:50:35.97 AS.reconfigure ASconfig
@17:50:36.04 AS.seek 0.5ml
@17:50:46.32 delay 1 second
@17:50:47.34 AFV.select :holdingCoil
@17:50:47.45 ASV.dial :waste1
@17:50:47.85 ATV.dial :air3
@17:50:49.22 AS.reconfigure ASslow1
@17:50:49.28 AS.seek 0.51ml
@17:50:51.59 delay 2 seconds
@17:50:53.62 ATV.dial :bleach
@17:50:54.27 AS.seek 0.81ml
@17:51:54.86 delay 3 seconds
@17:51:57.89 ATV.dial :air3
@17:51:58.47 delay 3 seconds
@17:52:01.54 AS.seek 0.835ml
@17:52:06.84 delay 3 seconds
@17:52:09.87 ATV.dial :bleach
@17:52:10.52 AS.seek 0.82ml
@17:52:13.85 delay 3 seconds
@17:52:16.88 ARV.dial :waste
@17:52:17.50 delay 3 seconds
@17:52:20.53 ATV.dial :ARV
@17:52:21.47 AS.reconfigure ASconfig
@17:52:21.52 AS.seek 0.47ml
@17:52:41.94 AFV.select :carrierSolution
@17:52:42.06 delay 2 seconds
@17:52:44.08 AS.seek 1ml
@17:53:14.85 delay 1 second
@17:53:15.87 AFV.select :holdingCoil
@17:53:15.98 delay 1 second
@17:53:17.00 AS.reconfigure ASfast
@17:53:17.06 AS.seek 0ml
@17:53:44.41 AFV.select :carrierSolution
@17:53:44.53 delay 2 seconds
@17:53:46.55 AS.reconfigure ASconfig
@17:53:46.60 AS.seek 1ml
@17:54:44.36 delay 1 second
@17:54:45.38 AFV.select :holdingCoil
@17:54:45.49 delay 1 second
@17:54:46.51 AS.reconfigure ASfast
@17:54:46.57 AS.seek 0ml
@17:55:13.91 AFV.select :carrierSolution
@17:55:14.03 delay 2 seconds
@17:55:16.05 AS.reconfigure ASconfig
@17:55:16.10 AS.seek 1ml
@17:56:13.86 delay 1 second
@17:56:14.88 AFV.select :holdingCoil
@17:56:14.99 delay 1 second
@17:56:16.01 AS.reconfigure ASfast
@17:56:16.07 AS.seek 0ml
@17:56:43.42 ARV.dial between carrier and air1
@17:56:43.94 ATV.dial :air3
@17:56:45.30 AS.reconfigure ASslow1
@17:56:45.36 AS.seek 0.01ml
@17:56:47.67 delay 2 seconds
@17:56:49.70 ATV.dial :bleach
@17:56:50.34 AS.seek 0.81ml
@17:59:31.49 delay 3 seconds
@17:59:34.52 ATV.dial :air3
@17:59:35.10 delay 3 seconds
@17:59:38.17 AS.seek 0.835ml
@17:59:43.48 delay 3 seconds
@17:59:46.51 ATV.dial :bleach
@17:59:47.14 AS.seek 0.82ml
@17:59:50.46 delay 3 seconds
@17:59:53.49 ATV.dial PCR
@17:59:53.90 AS.reconfigure ASconfig
@17:59:53.95 AS.seek 0ml
@18:00:41.38 PCR.program :bleachProfile
@18:00:41.46 PCR program should complete in < 2:45
@18:00:41.47 PCR.run 10:00
@18:03:37.70 PCR.off
@18:03:37.75 AFV.select :carrierSolution
@18:03:37.86 delay 2 seconds
@18:03:39.88 AS.seek 1ml
@18:04:37.66 delay 1 second
@18:04:38.68 AFV.select :holdingCoil
@18:04:38.79 delay 1 second
@18:04:39.81 AS.reconfigure ASfast
@18:04:39.87 AS.seek 0ml
@18:05:07.22 delay 3 seconds
@18:05:10.24 AFV.select :carrierSolution
@18:05:10.35 delay 2 seconds
@18:05:12.37 AS.reconfigure ASconfig
@18:05:12.43 AS.seek 1ml
@18:06:10.17 delay 1 second
@18:06:11.19 AFV.select :holdingCoil
@18:06:11.30 delay 1 second
@18:06:12.32 AS.reconfigure ASfast
@18:06:12.38 AS.seek 0ml
@18:06:39.72 delay 3 seconds
@18:06:42.74 AFV.select :carrierSolution
@18:06:42.85 delay 2 seconds
@18:06:44.87 AS.reconfigure ASconfig
@18:06:44.93 AS.seek 1ml
@18:07:42.69 delay 1 second
@18:07:43.71 AFV.select :holdingCoil
@18:07:43.82 delay 1 second
@18:07:44.84 AS.reconfigure ASfast
@18:07:44.90 AS.seek 0ml
@18:08:12.21 delay 3 seconds
@18:08:15.23 AFV.select :carrierSolution
@18:08:15.34 delay 2 seconds
@18:08:17.36 AS.reconfigure ASconfig
@18:08:17.43 AS.seek 1ml
@18:09:15.17 delay 1 second
@18:09:16.19 AFV.select :holdingCoil
@18:09:16.30 delay 1 second
@18:09:17.32 AS.reconfigure ASfast
@18:09:17.38 AS.seek 0ml
@18:09:44.72 delay 3 seconds
@18:09:47.74 AFV.select :carrierSolution
@18:09:47.85 delay 2 seconds
@18:09:49.87 AS.reconfigure ASconfig
@18:09:49.93 AS.seek 1ml
@18:10:47.71 delay 1 second
@18:10:48.73 AFV.select :holdingCoil
@18:10:48.84 delay 1 second
@18:10:49.86 AS.reconfigure ASnorm
@18:10:49.91 AS.seek 0ml
@18:11:40.63 Setting up PCR for AARPP4 -- writing /var/log/esp/AARPP421mar1718h.pcr
@18:11:40.64 Building PCR Slug from ASV with MM2 mix, PP4 primer
@18:11:40.67 Priming with carrier
@18:11:40.68 ATV.dial ARV
@18:11:41.35 ARV.dial :carrier
@18:11:41.70 AS.seek 0.5ml
@18:12:07.25 AFV.select :carrierSolution
@18:12:07.37 delay 2 seconds
@18:12:09.39 AS.reconfigure ASconfig
@18:12:09.46 AS.seek 1ml
@18:12:38.49 delay 1 second
@18:12:39.51 AFV.select :holdingCoil
@18:12:39.62 delay 1 second
@18:12:40.64 ATV.dial :waste
@18:12:42.40 AS.reconfigure ASnorm
@18:12:42.46 AS.seek 0ml
@18:13:33.17 AFV.select :carrierSolution
@18:13:33.29 delay 2 seconds
@18:13:35.31 AS.reconfigure ASconfig
@18:13:35.37 AS.seek 0.5ml
@18:14:04.41 delay 1 second
@18:14:05.44 AFV.select :holdingCoil
@18:14:05.56 Building Slug
@18:14:05.57 ATV.dial :ARV
@18:14:07.32 delay 2 seconds
@18:14:09.35 ARV.dial :air1
@18:14:09.75 delay 2 seconds
@18:14:11.82 AS.reconfigure ASslow2
@18:14:11.88 AS.seek 0.506ml
@18:14:18.21 delay 2 seconds
@18:14:20.24 ARV.dial :MM2
@18:14:21.79 delay 2 seconds
@18:14:23.81 Adding MM2
@18:14:23.89 AS.seek 0.524ml
@18:14:42.10 delay 25 seconds
@18:15:07.13 ARV.dial :PP4
@18:15:07.56 Adding PP4
@18:15:07.57 delay 2 seconds
@18:15:09.64 AS.seek 0.53ml
@18:15:16.17 delay 10 seconds
@18:15:26.21 ARV.dial :air3
@18:15:26.62 adding ARV air
@18:15:26.63 delay 2 seconds
@18:15:28.70 AS.seek 0.536ml
@18:15:35.17 delay 5 seconds
@18:15:40.20 ARV.dial :carrier
@18:15:41.52 delay 7 seconds
@18:15:48.60 AS.seek 0.594ml
@18:16:47.76 delay 5 seconds
@18:16:52.79 ATV.dial :ASV
@18:16:53.21 delay 2 seconds
@18:16:55.28 AS.seek 0.6ml
@18:17:01.53 delay 4 seconds
@18:17:05.56 ATV.dial ARV
@18:17:06.14 delay 2 seconds
@18:17:08.17 ARV.dial :carrier
@18:17:08.18 delay 2 seconds
@18:17:10.25 AS.seek 0.65ml
@18:18:00.69 delay 4 seconds
@18:18:04.72 ATV.dial PCR
@18:18:05.35 ASV.dial :waste1
@18:18:05.37 ARV.dial :air1
@18:18:05.78 Positioning Slug by 0.324ml
@18:18:05.87 AS.seek 0.326ml
@18:23:31.91 delay 5 seconds
@18:23:36.94 ATV.dial ARV
@18:23:37.75 ASV.dial :PCRlock
@18:23:38.16 delay 5 seconds
@18:23:43.19 Running AARPP4 Profile58CIQ2 thermocycling
@18:23:43.20 <PCR> PCR.program Profile58CIQ2
@18:23:43.32 Profile58CIQ2 should complete in < 1:36:15
@18:23:43.33 PCR.run 2:05:18.75
@19:44:18.73 PCR.off
@19:44:19.29 <MFBPCR> Running AARPP4 MeltProfile58 thermocycling
@19:44:19.30 <PCR> PCR.program MeltProfile58
@19:44:19.41 MeltProfile58 should complete in < 12:33
@19:44:19.42 PCR.run 20:41.25
@19:52:01.19 PCR.off
@19:52:02.15 <MFBPCR> PCR for AARPP4 completed -- closed /var/log/esp/AARPP421mar1718h.pcr
@19:52:02.23 Cleaning PCR
@19:52:02.24 AFV.select :carrierSolution
@19:52:02.36 delay 2 seconds
@19:52:04.38 AS.reconfigure ASconfig
@19:52:04.43 AS.seek 0.5ml
@19:52:14.74 delay 1 second
@19:52:15.76 AFV.select :holdingCoil
@19:52:15.87 ASV.dial :waste1
@19:52:16.27 ATV.dial :air3
@19:52:17.63 AS.reconfigure ASslow1
@19:52:17.69 AS.seek 0.51ml
@19:52:19.97 delay 2 seconds
@19:52:22.00 ATV.dial :bleach
@19:52:22.64 AS.seek 0.81ml
@19:53:23.22 delay 3 seconds
@19:53:26.25 ATV.dial :air3
@19:53:26.83 delay 3 seconds
@19:53:29.90 AS.seek 0.835ml
@19:53:35.24 delay 3 seconds
@19:53:38.27 ATV.dial :bleach
@19:53:38.91 AS.seek 0.82ml
@19:53:42.22 delay 3 seconds
@19:53:45.25 ARV.dial :waste
@19:53:45.88 delay 3 seconds
@19:53:48.91 ATV.dial :ARV
@19:53:49.85 AS.reconfigure ASconfig
@19:53:49.90 AS.seek 0.47ml
@19:54:10.32 AFV.select :carrierSolution
@19:54:10.44 delay 2 seconds
@19:54:12.46 AS.seek 1ml
@19:54:43.25 delay 1 second
@19:54:44.27 AFV.select :holdingCoil
@19:54:44.38 delay 1 second
@19:54:45.40 AS.reconfigure ASfast
@19:54:45.46 AS.seek 0ml
@19:55:12.80 AFV.select :carrierSolution
@19:55:12.92 delay 2 seconds
@19:55:14.94 AS.reconfigure ASconfig
@19:55:14.99 AS.seek 1ml
@19:56:12.75 delay 1 second
@19:56:13.77 AFV.select :holdingCoil
@19:56:13.88 delay 1 second
@19:56:14.90 AS.reconfigure ASfast
@19:56:14.96 AS.seek 0ml
@19:56:42.29 AFV.select :carrierSolution
@19:56:42.41 delay 2 seconds
@19:56:44.43 AS.reconfigure ASconfig
@19:56:44.48 AS.seek 1ml
@19:57:42.29 delay 1 second
@19:57:43.31 AFV.select :holdingCoil
@19:57:43.42 delay 1 second
@19:57:44.44 AS.reconfigure ASfast
@19:57:44.50 AS.seek 0ml
@19:58:11.83 ARV.dial between carrier and air1
@19:58:12.36 ATV.dial :air3
@19:58:13.72 AS.reconfigure ASslow1
@19:58:13.78 AS.seek 0.01ml
@19:58:16.08 delay 2 seconds
@19:58:18.11 ATV.dial :bleach
@19:58:18.75 AS.seek 0.81ml
@20:00:59.88 delay 3 seconds
@20:01:02.91 ATV.dial :air3
@20:01:03.49 delay 3 seconds
@20:01:06.56 AS.seek 0.835ml
@20:01:11.86 delay 3 seconds
@20:01:14.89 ATV.dial :bleach
@20:01:15.53 AS.seek 0.82ml
@20:01:18.96 delay 3 seconds
@20:01:21.99 ATV.dial PCR
@20:01:22.42 AS.reconfigure ASconfig
@20:01:22.88 AS.seek 0ml
@20:02:10.32 PCR.program :bleachProfile
@20:02:10.39 PCR program should complete in < 2:45
@20:02:10.40 PCR.run 10:00
@20:05:06.19 PCR.off
@20:05:06.23 AFV.select :carrierSolution
@20:05:06.34 delay 2 seconds
@20:05:08.36 AS.seek 1ml
@20:06:06.11 delay 1 second
@20:06:07.13 AFV.select :holdingCoil
@20:06:07.24 delay 1 second
@20:06:08.26 AS.reconfigure ASfast
@20:06:08.32 AS.seek 0ml
@20:06:35.66 delay 3 seconds
@20:06:38.68 AFV.select :carrierSolution
@20:06:38.79 delay 2 seconds
@20:06:40.81 AS.reconfigure ASconfig
@20:06:40.87 AS.seek 1ml
@20:07:38.68 delay 1 second
@20:07:39.70 AFV.select :holdingCoil
@20:07:39.81 delay 1 second
@20:07:40.83 AS.reconfigure ASfast
@20:07:40.89 AS.seek 0ml
@20:08:08.24 delay 3 seconds
@20:08:11.26 AFV.select :carrierSolution
@20:08:11.37 delay 2 seconds
@20:08:13.39 AS.reconfigure ASconfig
@20:08:13.45 AS.seek 1ml
@20:09:11.21 delay 1 second
@20:09:12.23 AFV.select :holdingCoil
@20:09:12.34 delay 1 second
@20:09:13.36 AS.reconfigure ASfast
@20:09:13.42 AS.seek 0ml
@20:09:40.77 delay 3 seconds
@20:09:43.79 AFV.select :carrierSolution
@20:09:43.90 delay 2 seconds
@20:09:45.92 AS.reconfigure ASconfig
@20:09:45.99 AS.seek 1ml
@20:10:43.74 delay 1 second
@20:10:44.76 AFV.select :holdingCoil
@20:10:44.87 delay 1 second
@20:10:45.89 AS.reconfigure ASfast
@20:10:45.95 AS.seek 0ml
@20:11:13.30 delay 3 seconds
@20:11:16.32 AFV.select :carrierSolution
@20:11:16.43 delay 2 seconds
@20:11:18.45 AS.reconfigure ASconfig
@20:11:18.51 AS.seek 1ml
@20:12:16.25 delay 1 second
@20:12:17.27 AFV.select :holdingCoil
@20:12:17.38 delay 1 second
@20:12:18.40 AS.reconfigure ASnorm
@20:12:18.45 AS.seek 0ml
@20:13:09.18 Setting up PCR for AALPP3 -- writing /var/log/esp/AALPP321mar1720h.pcr
@20:13:09.19 Building PCR Slug from ASV with MM2 mix, PP3 primer
@20:13:09.22 Priming with carrier
@20:13:09.23 ATV.dial ARV
@20:13:09.90 ARV.dial :carrier
@20:13:10.25 AS.seek 0.5ml
@20:13:35.80 AFV.select :carrierSolution
@20:13:35.92 delay 2 seconds
@20:13:37.94 AS.reconfigure ASconfig
@20:13:38.01 AS.seek 1ml
@20:14:07.04 delay 1 second
@20:14:08.06 AFV.select :holdingCoil
@20:14:08.17 delay 1 second
@20:14:09.19 ATV.dial :waste
@20:14:10.97 AS.reconfigure ASnorm
@20:14:11.03 AS.seek 0ml
@20:15:01.71 AFV.select :carrierSolution
@20:15:02.24 delay 2 seconds
@20:15:04.26 AS.reconfigure ASconfig
@20:15:04.32 AS.seek 0.5ml
@20:15:33.39 delay 1 second
@20:15:34.42 AFV.select :holdingCoil
@20:15:34.52 Building Slug
@20:15:34.53 ATV.dial :ARV
@20:15:36.31 delay 2 seconds
@20:15:38.33 ARV.dial :air1
@20:15:38.73 delay 2 seconds
@20:15:40.80 AS.reconfigure ASslow2
@20:15:40.86 AS.seek 0.506ml
@20:15:47.13 delay 2 seconds
@20:15:49.16 ARV.dial :MM2
@20:15:50.72 delay 2 seconds
@20:15:52.74 Adding MM2
@20:15:52.82 AS.seek 0.524ml
@20:16:11.08 delay 25 seconds
@20:16:36.11 ARV.dial :PP3
@20:16:36.50 Adding PP3
@20:16:36.52 delay 2 seconds
@20:16:38.59 AS.seek 0.53ml
@20:16:44.93 delay 10 seconds
@20:16:54.97 ARV.dial :air2
@20:16:55.38 adding ARV air
@20:16:55.40 delay 2 seconds
@20:16:57.47 AS.seek 0.536ml
@20:17:03.86 delay 5 seconds
@20:17:08.89 ARV.dial :carrier
@20:17:10.22 delay 7 seconds
@20:17:17.30 AS.seek 0.594ml
@20:18:15.89 delay 5 seconds
@20:18:20.92 ATV.dial :ASV
@20:18:21.33 delay 2 seconds
@20:18:23.40 AS.seek 0.6ml
@20:18:29.75 delay 4 seconds
@20:18:33.78 ATV.dial ARV
@20:18:34.36 delay 2 seconds
@20:18:36.39 ARV.dial :carrier
@20:18:36.40 delay 2 seconds
@20:18:38.47 AS.seek 0.65ml
@20:19:29.25 delay 4 seconds
@20:19:33.28 ATV.dial PCR
@20:19:33.91 ASV.dial :waste1
@20:19:33.93 ARV.dial :air1
@20:19:34.34 Positioning Slug by 0.324ml
@20:19:34.43 AS.seek 0.326ml
@20:25:00.65 delay 5 seconds
@20:25:05.68 ATV.dial ARV
@20:25:06.49 ASV.dial :PCRlock
@20:25:06.90 delay 5 seconds
@20:25:11.93 Running AALPP3 Profile58CIQ2 thermocycling
@20:25:11.94 <PCR> PCR.program Profile58CIQ2
@20:25:12.07 Profile58CIQ2 should complete in < 1:36:15
@20:25:12.08 PCR.run 2:05:18.75
@21:45:47.29 PCR.off
@21:45:47.84 <MFBPCR> Running AALPP3 MeltProfile58 thermocycling
@21:45:47.86 <PCR> PCR.program MeltProfile58
@21:45:47.96 MeltProfile58 should complete in < 12:33
@21:45:47.97 PCR.run 20:41.25
@21:53:14.46 PCR.off
@21:53:15.45 <MFBPCR> PCR for AALPP3 completed -- closed /var/log/esp/AALPP321mar1720h.pcr
@21:53:15.52 Cleaning PCR
@21:53:15.53 AFV.select :carrierSolution
@21:53:15.65 delay 2 seconds
@21:53:17.67 AS.reconfigure ASconfig
@21:53:17.73 AS.seek 0.5ml
@21:53:28.01 delay 1 second
@21:53:29.03 AFV.select :holdingCoil
@21:53:29.14 ASV.dial :waste1
@21:53:29.54 ATV.dial :air3
@21:53:30.91 AS.reconfigure ASslow1
@21:53:30.96 AS.seek 0.51ml
@21:53:33.37 delay 2 seconds
@21:53:35.40 ATV.dial :bleach
@21:53:36.03 AS.seek 0.81ml
@21:54:36.63 delay 3 seconds
@21:54:39.66 ATV.dial :air3
@21:54:40.24 delay 3 seconds
@21:54:43.31 AS.seek 0.835ml
@21:54:48.63 delay 3 seconds
@21:54:51.66 ATV.dial :bleach
@21:54:52.30 AS.seek 0.82ml
@21:54:55.62 delay 3 seconds
@21:54:58.65 ARV.dial :waste
@21:54:59.27 delay 3 seconds
@21:55:02.30 ATV.dial :ARV
@21:55:03.24 AS.reconfigure ASconfig
@21:55:03.29 AS.seek 0.47ml
@21:55:23.71 AFV.select :carrierSolution
@21:55:23.82 delay 2 seconds
@21:55:25.84 AS.seek 1ml
@21:55:56.63 delay 1 second
@21:55:57.65 AFV.select :holdingCoil
@21:55:57.76 delay 1 second
@21:55:58.78 AS.reconfigure ASfast
@21:55:58.84 AS.seek 0ml
@21:56:26.16 AFV.select :carrierSolution
@21:56:26.28 delay 2 seconds
@21:56:28.30 AS.reconfigure ASconfig
@21:56:28.36 AS.seek 1ml
@21:57:26.10 delay 1 second
@21:57:27.12 AFV.select :holdingCoil
@21:57:27.23 delay 1 second
@21:57:28.25 AS.reconfigure ASfast
@21:57:28.31 AS.seek 0ml
@21:57:55.63 AFV.select :carrierSolution
@21:57:55.75 delay 2 seconds
@21:57:58.14 AS.reconfigure ASconfig
@21:57:58.24 AS.seek 1ml
@21:58:55.99 delay 1 second
@21:58:57.01 AFV.select :holdingCoil
@21:58:57.12 delay 1 second
@21:58:58.14 AS.reconfigure ASfast
@21:58:58.20 AS.seek 0ml
@21:59:25.54 ARV.dial between carrier and air1
@21:59:26.07 ATV.dial :air3
@21:59:27.44 AS.reconfigure ASslow1
@21:59:27.50 AS.seek 0.01ml
@21:59:29.91 delay 2 seconds
@21:59:31.94 ATV.dial :bleach
@21:59:32.58 AS.seek 0.81ml
@22:02:13.72 delay 3 seconds
@22:02:16.75 ATV.dial :air3
@22:02:17.33 delay 3 seconds
@22:02:20.40 AS.seek 0.835ml
@22:02:25.80 delay 3 seconds
@22:02:28.83 ATV.dial :bleach
@22:02:29.47 AS.seek 0.82ml
@22:02:32.76 delay 3 seconds
@22:02:35.79 ATV.dial PCR
@22:02:36.22 AS.reconfigure ASconfig
@22:02:36.27 AS.seek 0ml
@22:03:23.68 PCR.program :bleachProfile
@22:03:23.75 PCR program should complete in < 2:45
@22:03:23.76 PCR.run 10:00
@22:06:22.59 PCR.off
@22:06:22.63 AFV.select :carrierSolution
@22:06:22.74 delay 2 seconds
@22:06:24.76 AS.seek 1ml
@22:07:22.52 delay 1 second
@22:07:23.54 AFV.select :holdingCoil
@22:07:23.65 delay 1 second
@22:07:24.67 AS.reconfigure ASfast
@22:07:24.73 AS.seek 0ml
@22:07:52.05 delay 3 seconds
@22:07:55.07 AFV.select :carrierSolution
@22:07:55.18 delay 2 seconds
@22:07:57.20 AS.reconfigure ASconfig
@22:07:57.27 AS.seek 1ml
@22:08:55.02 delay 1 second
@22:08:56.04 AFV.select :holdingCoil
@22:08:56.15 delay 1 second
@22:08:57.17 AS.reconfigure ASfast
@22:08:57.23 AS.seek 0ml
@22:09:24.55 delay 3 seconds
@22:09:27.57 AFV.select :carrierSolution
@22:09:27.68 delay 2 seconds
@22:09:29.70 AS.reconfigure ASconfig
@22:09:29.76 AS.seek 1ml
@22:10:27.52 delay 1 second
@22:10:28.54 AFV.select :holdingCoil
@22:10:28.65 delay 1 second
@22:10:29.67 AS.reconfigure ASfast
@22:10:29.73 AS.seek 0ml
@22:10:57.08 delay 3 seconds
@22:11:00.10 AFV.select :carrierSolution
@22:11:00.21 delay 2 seconds
@22:11:02.23 AS.reconfigure ASconfig
@22:11:02.29 AS.seek 1ml
@22:12:00.08 delay 1 second
@22:12:01.10 AFV.select :holdingCoil
@22:12:01.62 delay 1 second
@22:12:02.64 AS.reconfigure ASfast
@22:12:02.70 AS.seek 0ml
@22:12:30.04 delay 3 seconds
@22:12:33.06 AFV.select :carrierSolution
@22:12:33.17 delay 2 seconds
@22:12:35.19 AS.reconfigure ASconfig
@22:12:35.25 AS.seek 1ml
@22:13:33.04 delay 1 second
@22:13:34.06 AFV.select :holdingCoil
@22:13:34.17 delay 1 second
@22:13:35.19 AS.reconfigure ASnorm
@22:13:35.25 AS.seek 0ml
@22:14:25.96 Setting up PCR for ACOPP5 -- writing /var/log/esp/ACOPP521mar1722h.pcr
@22:14:25.98 Building PCR Slug from ASV with MM3 mix, PP5 primer
@22:14:26.00 Priming with carrier
@22:14:26.01 ATV.dial ARV
@22:14:26.68 ARV.dial :carrier
@22:14:27.04 AS.seek 0.5ml
@22:14:52.62 AFV.select :carrierSolution
@22:14:52.75 delay 2 seconds
@22:14:54.77 AS.reconfigure ASconfig
@22:14:54.84 AS.seek 1ml
@22:15:23.87 delay 1 second
@22:15:24.89 AFV.select :holdingCoil
@22:15:25.00 delay 1 second
@22:15:26.02 ATV.dial :waste
@22:15:27.80 AS.reconfigure ASnorm
@22:15:27.86 AS.seek 0ml
@22:16:18.55 AFV.select :carrierSolution
@22:16:18.68 delay 2 seconds
@22:16:20.70 AS.reconfigure ASconfig
@22:16:20.76 AS.seek 0.5ml
@22:16:49.83 delay 1 second
@22:16:50.85 AFV.select :holdingCoil
@22:16:50.96 Building Slug
@22:16:50.97 ATV.dial :ARV
@22:16:52.73 delay 2 seconds
@22:16:54.76 ARV.dial :air1
@22:16:55.16 delay 2 seconds
@22:16:57.23 AS.reconfigure ASslow2
@22:16:57.29 AS.seek 0.506ml
@22:17:03.55 delay 2 seconds
@22:17:05.58 ARV.dial :MM3
@22:17:06.66 delay 2 seconds
@22:17:08.69 Adding MM3
@22:17:08.77 AS.seek 0.524ml
@22:17:27.30 delay 25 seconds
@22:17:52.33 ARV.dial :PP5
@22:17:52.74 Adding PP5
@22:17:52.76 delay 2 seconds
@22:17:54.83 AS.seek 0.53ml
@22:18:01.02 delay 10 seconds
@22:18:11.06 ARV.dial :air3
@22:18:11.47 adding ARV air
@22:18:11.48 delay 2 seconds
@22:18:13.55 AS.seek 0.536ml
@22:18:20.08 delay 5 seconds
@22:18:25.11 ARV.dial :carrier
@22:18:26.41 delay 7 seconds
@22:18:33.48 AS.seek 0.594ml
@22:19:32.04 delay 5 seconds
@22:19:37.07 ATV.dial :ASV
@22:19:37.49 delay 2 seconds
@22:19:39.56 AS.seek 0.6ml
@22:19:45.76 delay 4 seconds
@22:19:49.79 ATV.dial ARV
@22:19:50.48 delay 2 seconds
@22:19:52.51 ARV.dial :carrier
@22:19:52.52 delay 2 seconds
@22:19:54.59 AS.seek 0.65ml
@22:20:45.24 delay 4 seconds
@22:20:49.27 ATV.dial PCR
@22:20:49.91 ASV.dial :waste1
@22:20:49.93 ARV.dial :air1
@22:20:50.37 Positioning Slug by 0.324ml
@22:20:50.45 AS.seek 0.326ml
@22:26:16.77 delay 5 seconds
@22:26:21.80 ATV.dial ARV
@22:26:22.62 ASV.dial :PCRlock
@22:26:23.02 delay 5 seconds
@22:26:28.05 Running ACOPP5 ProfileIPc thermocycling
@22:26:28.06 <PCR> PCR.program ProfileIPc
@22:26:28.18 ProfileIPc should complete in < 1:26:35
@22:26:28.19 PCR.run 1:53:13.75
@23:34:43.09 PCR.off
@23:34:43.65 <MFBPCR> PCR for ACOPP5 completed -- closed /var/log/esp/ACOPP521mar1722h.pcr
@23:34:43.72 Cleaning PCR
@23:34:43.73 AFV.select :carrierSolution
@23:34:43.86 delay 2 seconds
@23:34:45.88 AS.reconfigure ASconfig
@23:34:45.93 AS.seek 0.5ml
@23:34:56.24 delay 1 second
@23:34:57.26 AFV.select :holdingCoil
@23:34:57.37 ASV.dial :waste1
@23:34:57.76 ATV.dial :air3
@23:34:59.11 AS.reconfigure ASslow1
@23:34:59.16 AS.seek 0.51ml
@23:35:01.44 delay 2 seconds
@23:35:03.46 ATV.dial :bleach
@23:35:04.10 AS.seek 0.81ml
@23:36:04.68 delay 3 seconds
@23:36:07.71 ATV.dial :air3
@23:36:08.29 delay 3 seconds
@23:36:11.36 AS.seek 0.835ml
@23:36:16.66 delay 3 seconds
@23:36:19.69 ATV.dial :bleach
@23:36:20.33 AS.seek 0.82ml
@23:36:23.65 delay 3 seconds
@23:36:26.68 ARV.dial :waste
@23:36:27.31 delay 3 seconds
@23:36:30.34 ATV.dial :ARV
@23:36:31.27 AS.reconfigure ASconfig
@23:36:31.32 AS.seek 0.47ml
@23:36:51.74 AFV.select :carrierSolution
@23:36:51.86 delay 2 seconds
@23:36:53.88 AS.seek 1ml
@23:37:24.65 delay 1 second
@23:37:25.67 AFV.select :holdingCoil
@23:37:25.78 delay 1 second
@23:37:26.80 AS.reconfigure ASfast
@23:37:26.86 AS.seek 0ml
@23:37:54.20 AFV.select :carrierSolution
@23:37:54.32 delay 2 seconds
@23:37:56.34 AS.reconfigure ASconfig
@23:37:56.39 AS.seek 1ml
@23:38:54.13 delay 1 second
@23:38:55.15 AFV.select :holdingCoil
@23:38:55.26 delay 1 second
@23:38:56.67 AS.reconfigure ASfast
@23:38:56.75 AS.seek 0ml
@23:39:24.07 AFV.select :carrierSolution
@23:39:24.19 delay 2 seconds
@23:39:26.21 AS.reconfigure ASconfig
@23:39:26.27 AS.seek 1ml
@23:40:24.05 delay 1 second
@23:40:25.07 AFV.select :holdingCoil
@23:40:25.18 delay 1 second
@23:40:26.20 AS.reconfigure ASfast
@23:40:26.26 AS.seek 0ml
@23:40:53.59 ARV.dial between carrier and air1
@23:40:54.11 ATV.dial :air3
@23:40:55.47 AS.reconfigure ASslow1
@23:40:55.53 AS.seek 0.01ml
@23:40:57.93 delay 2 seconds
@23:40:59.96 ATV.dial :bleach
@23:41:00.60 AS.seek 0.81ml
@23:43:41.77 delay 3 seconds
@23:43:44.80 ATV.dial :air3
@23:43:45.38 delay 3 seconds
@23:43:48.45 AS.seek 0.835ml
@23:43:53.77 delay 3 seconds
@23:43:56.80 ATV.dial :bleach
@23:43:57.44 AS.seek 0.82ml
@23:44:00.76 delay 3 seconds
@23:44:03.79 ATV.dial PCR
@23:44:04.22 AS.reconfigure ASconfig
@23:44:04.27 AS.seek 0ml
@23:44:51.70 PCR.program :bleachProfile
@23:44:51.78 PCR program should complete in < 2:45
@23:44:51.79 PCR.run 10:00
@23:47:47.58 PCR.off
@23:47:47.62 AFV.select :carrierSolution
@23:47:47.73 delay 2 seconds
@23:47:49.75 AS.seek 1ml
@23:48:47.54 delay 1 second
@23:48:48.56 AFV.select :holdingCoil
@23:48:48.67 delay 1 second
@23:48:49.69 AS.reconfigure ASfast
@23:48:49.75 AS.seek 0ml
@23:49:17.10 delay 3 seconds
@23:49:20.13 AFV.select :carrierSolution
@23:49:20.23 delay 2 seconds
@23:49:22.25 AS.reconfigure ASconfig
@23:49:22.32 AS.seek 1ml
@23:50:20.06 delay 1 second
@23:50:21.08 AFV.select :holdingCoil
@23:50:21.19 delay 1 second
@23:50:22.21 AS.reconfigure ASfast
@23:50:22.27 AS.seek 0ml
@23:50:49.59 delay 3 seconds
@23:50:52.61 AFV.select :carrierSolution
@23:50:52.72 delay 2 seconds
@23:50:54.74 AS.reconfigure ASconfig
@23:50:54.80 AS.seek 1ml
@23:51:52.59 delay 1 second
@23:51:53.61 AFV.select :holdingCoil
@23:51:53.72 delay 1 second
@23:51:54.74 AS.reconfigure ASfast
@23:51:54.80 AS.seek 0ml
@23:52:22.13 delay 3 seconds
@23:52:25.15 AFV.select :carrierSolution
@23:52:25.26 delay 2 seconds
@23:52:27.63 AS.reconfigure ASconfig
@23:52:27.74 AS.seek 1ml
@23:53:25.48 delay 1 second
@23:53:26.50 AFV.select :holdingCoil
@23:53:26.61 delay 1 second
@23:53:27.63 AS.reconfigure ASfast
@23:53:27.69 AS.seek 0ml
@23:53:55.04 delay 3 seconds
@23:53:58.06 AFV.select :carrierSolution
@23:53:58.17 delay 2 seconds
@23:54:00.19 AS.reconfigure ASconfig
@23:54:00.26 AS.seek 1ml
@23:54:57.99 delay 1 second
@23:54:59.01 AFV.select :holdingCoil
@23:54:59.12 delay 1 second
@23:55:00.14 AS.reconfigure ASnorm
@23:55:00.20 AS.seek 0ml
@23:55:50.96 Setting up PCR for AIPc -- writing /var/log/esp/AIPc21mar1723h.pcr
@23:55:50.97 Building PCR Slug from ASV with MM3 mix, PP6 primer
@23:55:51.00 Priming with carrier
@23:55:51.01 ATV.dial ARV
@23:55:51.68 ARV.dial :carrier
@23:55:52.04 AS.seek 0.5ml
@23:56:17.60 AFV.select :carrierSolution
@23:56:17.73 delay 2 seconds
@23:56:19.75 AS.reconfigure ASconfig
@23:56:19.82 AS.seek 1ml
@23:56:48.85 delay 1 second
@23:56:49.87 AFV.select :holdingCoil
@23:56:49.98 delay 1 second
@23:56:51.00 ATV.dial :waste
@23:56:52.78 AS.reconfigure ASnorm
@23:56:52.84 AS.seek 0ml
@23:57:43.59 AFV.select :carrierSolution
@23:57:43.72 delay 2 seconds
@23:57:45.74 AS.reconfigure ASconfig
@23:57:45.80 AS.seek 0.5ml
@23:58:14.84 delay 1 second
@23:58:15.86 AFV.select :holdingCoil
@23:58:15.97 Building Slug
@23:58:15.98 ATV.dial :ARV
@23:58:17.73 delay 2 seconds
@23:58:19.76 ARV.dial :air1
@23:58:20.15 delay 2 seconds
@23:58:22.22 AS.reconfigure ASslow2
@23:58:22.28 AS.seek 0.506ml
@23:58:28.49 delay 2 seconds
@23:58:30.52 ARV.dial :MM3
@23:58:31.60 delay 2 seconds
@23:58:33.63 Adding MM3
@23:58:33.71 AS.seek 0.524ml
@23:58:52.12 delay 25 seconds
@23:59:17.15 ARV.dial :PP6
@23:59:17.54 Adding PP6
@23:59:17.56 delay 2 seconds
@23:59:19.63 AS.seek 0.53ml
@23:59:25.87 delay 10 seconds
@23:59:35.91 ARV.dial :air1
@23:59:36.76 adding ARV air
@23:59:36.78 delay 2 seconds
@23:59:38.85 AS.seek 0.536ml
@23:59:45.29 delay 5 seconds
@23:59:50.32 ARV.dial :carrier
@23:59:50.73 delay 7 seconds
@23:59:57.80 AS.seek 0.594ml
@00:00:56.48CET18-Mar-21 delay 5 seconds
@00:01:01.51 ATV.dial :ASV
@00:01:01.93 delay 2 seconds
@00:01:04.00 AS.seek 0.6ml
@00:01:10.26 delay 4 seconds
@00:01:14.29 ATV.dial ARV
@00:01:14.85 delay 2 seconds
@00:01:16.88 ARV.dial :carrier
@00:01:16.89 delay 2 seconds
@00:01:18.96 AS.seek 0.65ml
@00:02:09.68 delay 4 seconds
@00:02:13.71 ATV.dial PCR
@00:02:14.35 ASV.dial :waste1
@00:02:14.37 ARV.dial :air1
@00:02:14.78 Positioning Slug by 0.324ml
@00:02:14.87 AS.seek 0.326ml
@00:07:41.18 delay 5 seconds
@00:07:46.21 ATV.dial ARV
@00:07:47.02 ASV.dial :PCRlock
@00:07:47.43 delay 5 seconds
@00:07:52.46 Running AIPc ProfileIPc thermocycling
@00:07:52.47 <PCR> PCR.program ProfileIPc
@00:07:52.59 ProfileIPc should complete in < 1:26:35
@00:07:52.60 PCR.run 1:53:13.75
@01:16:08.28 PCR.off
@01:16:08.85 <MFBPCR> PCR for AIPc completed -- closed /var/log/esp/AIPc21mar1723h.pcr
@01:16:08.87 Full MFB Decontamination
@01:16:08.89 Decon extraction column and decon extSrc
@01:16:08.91 SPE.hold 2:46:40,55C
@01:16:08.97 ASV.dial :SPEdelivery
@01:16:09.65 delay 20 seconds
@01:16:29.69 AFV.select :carrierSolution
@01:16:29.79 delay 2 seconds
@01:16:31.81 AS.reconfigure ASconfig
@01:16:31.86 AS.seek 0.5ml
@01:16:42.21 delay 1 second
@01:16:43.24 AFV.select :holdingCoil
@01:16:43.34 ATV.dial :air1
@01:16:45.60 AS.reconfigure ASslow1
@01:16:45.66 AS.seek 0.52ml
@01:16:50.00 ATV.dial :ASV
@01:16:51.76 ASV.dial :SPErecovery
@01:16:52.46 AS.seek 0.62ml
@01:17:12.85 ATV.dial :waste
@01:17:15.05 AS.reconfigure ASnorm
@01:17:15.11 AS.seek 0ml
@01:17:46.70 deconEluateRecoveryLine extSrc
@01:17:46.71 ATV.dial :waste
@01:17:46.75 AS.seek 0ml
@01:17:46.84 ATV.dial :air3
@01:17:47.60 AS.reconfigure ASslow1
@01:17:47.66 AS.seek 0.01ml
@01:17:50.06 delay 2 seconds
@01:17:52.09 ATV.dial :bleach
@01:17:52.59 AS.seek 0.31ml
@01:18:53.21 delay 3 seconds
@01:18:56.24 ATV.dial :air3
@01:18:56.87 delay 3 seconds
@01:18:59.94 AS.seek 0.335ml
@01:19:05.26 delay 3 seconds
@01:19:08.69 ATV.dial :bleach
@01:19:09.37 AS.seek 0.32ml
@01:19:12.73 delay 3 seconds
@01:19:15.76 ATV.dial :extSrc
@01:19:16.44 AS.seek 0ml
@01:20:21.06 AFV.select :carrierSolution
@01:20:21.19 delay 2 seconds
@01:20:23.21 AS.reconfigure ASconfig
@01:20:23.27 AS.seek 0.5ml
@01:20:52.31 delay 1 second
@01:20:53.33 AFV.select :holdingCoil
@01:20:53.44 ATV.dial :extSrc
@01:20:53.46 AS.reconfigure ASslow1
@01:20:53.53 AS.seek 0ml
@01:22:34.35 AFV.select :carrierSolution
@01:22:34.48 delay 2 seconds
@01:22:36.50 AS.reconfigure ASconfig
@01:22:36.56 AS.seek 0.5ml
@01:23:05.60 delay 1 second
@01:23:06.62 AFV.select :holdingCoil
@01:23:06.73 ATV.dial :extSrc
@01:23:06.75 AS.reconfigure ASslow1
@01:23:06.82 AS.seek 0ml
@01:24:47.64 AFV.select :carrierSolution
@01:24:47.76 delay 2 seconds
@01:24:49.78 AS.reconfigure ASconfig
@01:24:49.84 AS.seek 0.5ml
@01:25:18.84 delay 1 second
@01:25:19.86 AFV.select :holdingCoil
@01:25:19.97 ATV.dial :extSrc
@01:25:19.99 AS.reconfigure ASslow1
@01:25:20.06 AS.seek 0ml
@01:27:00.90 AFV.select :carrierSolution
@01:27:01.02 delay 2 seconds
@01:27:03.04 AS.reconfigure ASconfig
@01:27:03.10 AS.seek 0.5ml
@01:27:32.14 delay 1 second
@01:27:33.16 AFV.select :holdingCoil
@01:27:33.27 ATV.dial :extSrc
@01:27:33.29 AS.reconfigure ASslow1
@01:27:33.36 AS.seek 0ml
@01:29:14.18 ATV.dial :air2
@01:29:15.59 AS.reconfigure ASslow0
@01:29:15.65 AS.seek 0.5ml
@01:30:06.28 ATV.dial :extSrc
@01:30:07.87 AS.seek 0.02ml
@01:30:56.43 delay 1 second
@01:30:57.46 ATV.dial :waste
@01:30:58.83 AS.reconfigure ASconfig
@01:30:58.88 AS.seek 0ml
@01:31:00.31 AFV.select :holdingCoil
@01:31:00.32 ATV.dial :waste
@01:31:00.35 AS.seek 0ml
@01:31:00.43 AFV.select :carrierSolution
@01:31:00.54 delay 2 seconds
@01:31:02.56 AS.seek 0.5ml
@01:31:31.60 delay 1 second
@01:31:32.62 AFV.select :holdingCoil
@01:31:32.73 ATV.dial :air3
@01:31:33.62 AS.reconfigure ASslow1
@01:31:33.68 AS.seek 0.52ml
@01:31:37.98 ATV.dial :air3
@01:31:38.07 AS.seek 0.53ml
@01:31:40.35 delay 2 seconds
@01:31:42.38 ATV.dial :bleach
@01:31:42.87 AS.seek 0.71ml
@01:32:19.34 delay 3 seconds
@01:32:22.37 ATV.dial :air3
@01:32:22.96 delay 3 seconds
@01:32:26.03 AS.seek 0.735ml
@01:32:31.34 delay 3 seconds
@01:32:34.37 ATV.dial :bleach
@01:32:35.02 AS.seek 0.72ml
@01:32:38.32 delay 3 seconds
@01:32:41.35 ATV.dial ASV
@01:32:42.46 ASV.dial :SPErecovery
@01:32:42.55 AS.seek 0.365ml
@01:33:54.15 ATV.dial :waste
@01:33:56.19 AS.reconfigure ASnorm
@01:33:56.25 AS.seek 0ml
@01:34:15.01 ATV.dial :air3
@01:34:15.92 AS.reconfigure ASslow1
@01:34:15.98 AS.seek 0.01ml
@01:34:18.35 delay 2 seconds
@01:34:20.38 ATV.dial :bleach
@01:34:20.87 AS.seek 0.58ml
@01:36:15.85 delay 3 seconds
@01:36:18.88 ATV.dial :air3
@01:36:19.46 delay 3 seconds
@01:36:22.53 AS.seek 0.605ml
@01:36:27.96 delay 3 seconds
@01:36:30.99 ATV.dial :bleach
@01:36:31.63 AS.seek 0.59ml
@01:36:34.92 delay 3 seconds
@01:36:37.95 ATV.dial :ASV
@01:36:39.09 ASV.dial :wasteR
@01:36:39.49 AS.seek 0ml
@01:38:38.40 delay 1:00
@01:39:38.44 ATV.dial ASV
@01:39:38.45 AFV.select :carrierSolution
@01:39:38.57 delay 2 seconds
@01:39:40.59 AS.reconfigure ASconfig
@01:39:40.65 AS.seek 1ml
@01:40:38.40 delay 1 second
@01:40:39.42 AFV.select :holdingCoil
@01:40:39.53 delay 1 second
@01:40:40.55 ASV.dial :wasteR
@01:40:40.56 AS.reconfigure ASslow1
@01:40:40.64 AS.seek 0ml
@01:44:02.01 delay 10 seconds
@01:44:12.05 ATV.dial :air3
@01:44:14.10 AS.seek 0.01ml
@01:44:16.45 delay 2 seconds
@01:44:18.48 ATV.dial :bleach
@01:44:19.13 AS.seek 0.58ml
@01:46:14.04 delay 3 seconds
@01:46:17.07 ATV.dial :air3
@01:46:17.67 delay 3 seconds
@01:46:20.75 AS.seek 0.605ml
@01:46:26.04 delay 3 seconds
@01:46:29.07 ATV.dial :bleach
@01:46:29.73 AS.seek 0.59ml
@01:46:33.04 delay 3 seconds
@01:46:36.07 ATV.dial ASV
@01:46:37.18 ASV.dial :SPEdelivery
@01:46:37.57 AS.seek 0ml
@01:48:36.45 delay 1:00
@01:49:36.48 AFV.select :carrierSolution
@01:49:36.59 delay 2 seconds
@01:49:38.61 AS.reconfigure ASconfig
@01:49:38.67 AS.seek 1ml
@01:50:36.42 delay 1 second
@01:50:37.44 AFV.select :holdingCoil
@01:50:37.55 delay 1 second
@01:50:38.57 ATV.dial ASV
@01:50:38.58 ASV.dial :SPEdelivery
@01:50:38.61 AS.reconfigure ASslow1
@01:50:38.67 AS.seek 0ml
@01:54:00.06 delay 10 seconds
@01:54:10.10 ATV.dial :air2
@01:54:11.01 AS.reconfigure ASslow0
@01:54:11.07 AS.seek 0.8ml
@01:55:31.84 delay 3 seconds
@01:55:34.87 ATV.dial :ASV
@01:55:35.92 ASV.dial :SPEdelivery
@01:55:35.94 AS.reconfigure ASslow1
@01:55:36.01 AS.seek 0.03ml
@01:58:11.14 delay 20 seconds
@01:58:31.18 ATV.dial :waste,via: :air3
@01:58:33.04 AS.reconfigure ASconfig
@01:58:33.09 AS.seek 0ml
@01:58:35.12 SPE.coast
@01:58:35.20 Removing Bleach from Mixing Coil
@01:58:35.21 AFV.select :carrierSolution
@01:58:35.34 delay 2 seconds
@01:58:37.36 AS.seek 0.5ml
@01:59:06.39 delay 1 second
@01:59:07.41 AFV.select :holdingCoil
@01:59:07.52 ATV.dial :ASV
@01:59:09.48 ASV.dial :SPErecovery
@01:59:10.15 AS.reconfigure ASslow1
@01:59:10.21 AS.seek 0.73ml
@01:59:56.73 ASV.dial :wasteD
@01:59:57.35 AS.reconfigure ASnorm
@01:59:57.40 AS.seek 0ml
@02:00:34.51 AFV.select :carrierSolution
@02:00:34.63 delay 2 seconds
@02:00:36.65 AS.reconfigure ASconfig
@02:00:36.70 AS.seek 0.5ml
@02:01:05.78 delay 1 second
@02:01:06.80 AFV.select :holdingCoil
@02:01:06.91 ATV.dial ASV
@02:01:06.92 ASV.dial :SPErecovery
@02:01:07.59 AS.reconfigure ASslow0
@02:01:07.65 AS.seek 0.3ml
@02:01:28.04 ATV.dial :air2
@02:01:28.98 AS.reconfigure ASslow1
@02:01:29.04 AS.seek 0.32ml
@02:01:33.29 ATV.dial ASV
@02:01:34.38 AS.reconfigure ASslow0
@02:01:34.44 AS.seek 0.57ml
@02:01:59.92 ASV.dial :wasteD
@02:02:00.54 AS.reconfigure ASnorm
@02:02:00.60 AS.seek 0ml
@02:02:29.67 AFV.select :carrierSolution
@02:02:29.79 delay 2 seconds
@02:02:31.81 AS.reconfigure ASconfig
@02:02:31.87 AS.seek 0.5ml
@02:03:00.90 delay 1 second
@02:03:01.92 AFV.select :holdingCoil
@02:03:02.03 ATV.dial ASV
@02:03:02.04 ASV.dial :SPErecovery
@02:03:02.70 AS.reconfigure ASslow0
@02:03:02.76 AS.seek 0.3ml
@02:03:23.25 ATV.dial :air2
@02:03:24.35 AS.reconfigure ASslow1
@02:03:24.41 AS.seek 0.32ml
@02:03:28.72 ATV.dial ASV
@02:03:29.82 AS.reconfigure ASslow0
@02:03:29.88 AS.seek 0.57ml
@02:03:55.37 ASV.dial :wasteD
@02:03:55.99 AS.reconfigure ASnorm
@02:03:56.04 AS.seek 0ml
@02:04:25.12 ATV.dial :air2
@02:04:26.18 AS.reconfigure ASslow0
@02:04:26.23 AS.seek 0.3ml
@02:04:56.81 ATV.dial :ASV
@02:04:57.86 ASV.dial :wasteD
@02:04:57.88 AS.reconfigure ASconfig
@02:04:57.95 AS.seek 0.05ml
@02:05:12.62 ATV.dial :waste
@02:05:14.63 AS.seek 0ml
@02:05:17.82 ASV.dial :SPEdelivery
@02:05:18.89 Cleaning PCR
@02:05:18.90 AFV.select :carrierSolution
@02:05:19.02 delay 2 seconds
@02:05:21.04 AS.seek 0.5ml
@02:05:50.50 delay 1 second
@02:05:51.52 AFV.select :holdingCoil
@02:05:51.63 ASV.dial :waste1
@02:05:52.01 ATV.dial :air3
@02:05:52.74 AS.reconfigure ASslow1
@02:05:52.80 AS.seek 0.51ml
@02:05:55.09 delay 2 seconds
@02:05:57.12 ATV.dial :bleach
@02:05:57.59 AS.seek 0.81ml
@02:06:58.17 delay 3 seconds
@02:07:01.20 ATV.dial :air3
@02:07:01.78 delay 3 seconds
@02:07:04.86 AS.seek 0.835ml
@02:07:10.17 delay 3 seconds
@02:07:13.20 ATV.dial :bleach
@02:07:13.84 AS.seek 0.82ml
@02:07:17.14 delay 3 seconds
@02:07:20.17 ARV.dial :waste
@02:07:20.79 delay 3 seconds
@02:07:23.82 ATV.dial :ARV
@02:07:24.76 AS.reconfigure ASconfig
@02:07:24.82 AS.seek 0.47ml
@02:07:45.23 AFV.select :carrierSolution
@02:07:45.35 delay 2 seconds
@02:07:47.37 AS.seek 1ml
@02:08:18.15 delay 1 second
@02:08:19.17 AFV.select :holdingCoil
@02:08:19.28 delay 1 second
@02:08:20.30 AS.reconfigure ASfast
@02:08:20.36 AS.seek 0ml
@02:08:47.70 AFV.select :carrierSolution
@02:08:47.82 delay 2 seconds
@02:08:49.84 AS.reconfigure ASconfig
@02:08:49.90 AS.seek 1ml
@02:09:47.68 delay 1 second
@02:09:48.70 AFV.select :holdingCoil
@02:09:48.81 delay 1 second
@02:09:49.83 AS.reconfigure ASfast
@02:09:49.89 AS.seek 0ml
@02:10:17.23 AFV.select :carrierSolution
@02:10:17.35 delay 2 seconds
@02:10:19.37 AS.reconfigure ASconfig
@02:10:19.43 AS.seek 1ml
@02:11:17.25 delay 1 second
@02:11:18.27 AFV.select :holdingCoil
@02:11:18.38 delay 1 second
@02:11:19.40 AS.reconfigure ASfast
@02:11:19.46 AS.seek 0ml
@02:11:46.79 ARV.dial between carrier and air1
@02:11:47.30 ATV.dial :air3
@02:11:48.67 AS.reconfigure ASslow1
@02:11:48.73 AS.seek 0.01ml
@02:11:51.07 delay 2 seconds
@02:11:53.10 ATV.dial :bleach
@02:11:53.74 AS.seek 0.81ml
@02:14:34.87 delay 3 seconds
@02:14:37.90 ATV.dial :air3
@02:14:38.48 delay 3 seconds
@02:14:41.55 AS.seek 0.835ml
@02:14:46.90 delay 3 seconds
@02:14:49.93 ATV.dial :bleach
@02:14:50.57 AS.seek 0.82ml
@02:14:53.89 delay 3 seconds
@02:14:56.92 ATV.dial PCR
@02:14:57.33 AS.reconfigure ASconfig
@02:14:57.38 AS.seek 0ml
@02:15:44.79 PCR.program :bleachProfile
@02:15:44.87 PCR program should complete in < 2:45
@02:15:44.88 PCR.run 10:00
@02:18:47.14 PCR.off
@02:18:47.18 AFV.select :carrierSolution
@02:18:47.29 delay 2 seconds
@02:18:49.31 AS.seek 1ml
@02:19:47.06 delay 1 second
@02:19:48.08 AFV.select :holdingCoil
@02:19:48.19 delay 1 second
@02:19:49.21 AS.reconfigure ASfast
@02:19:49.27 AS.seek 0ml
@02:20:16.62 delay 3 seconds
@02:20:19.64 AFV.select :carrierSolution
@02:20:19.75 delay 2 seconds
@02:20:21.77 AS.reconfigure ASconfig
@02:20:21.83 AS.seek 1ml
@02:21:19.56 delay 1 second
@02:21:20.58 AFV.select :holdingCoil
@02:21:20.69 delay 1 second
@02:21:21.71 AS.reconfigure ASfast
@02:21:21.77 AS.seek 0ml
@02:21:49.12 delay 3 seconds
@02:21:52.14 AFV.select :carrierSolution
@02:21:52.25 delay 2 seconds
@02:21:54.27 AS.reconfigure ASconfig
@02:21:54.33 AS.seek 1ml
@02:22:52.07 delay 1 second
@02:22:53.09 AFV.select :holdingCoil
@02:22:53.20 delay 1 second
@02:22:54.22 AS.reconfigure ASfast
@02:22:54.28 AS.seek 0ml
@02:23:21.62 delay 3 seconds
@02:23:24.64 AFV.select :carrierSolution
@02:23:24.75 delay 2 seconds
@02:23:26.77 AS.reconfigure ASconfig
@02:23:26.83 AS.seek 1ml
@02:24:24.57 delay 1 second
@02:24:25.59 AFV.select :holdingCoil
@02:24:25.70 delay 1 second
@02:24:26.72 AS.reconfigure ASfast
@02:24:26.78 AS.seek 0ml
@02:24:54.12 delay 3 seconds
@02:24:57.14 AFV.select :carrierSolution
@02:24:57.25 delay 2 seconds
@02:24:59.27 AS.reconfigure ASconfig
@02:24:59.33 AS.seek 1ml
@02:25:57.09 delay 1 second
@02:25:58.11 AFV.select :holdingCoil
@02:25:58.22 delay 1 second
@02:25:59.24 AS.reconfigure ASnorm
@02:25:59.29 AS.seek 0ml
@02:26:49.99 AFV.select :carrierSolution
@02:26:50.11 delay 2 seconds
@02:26:52.13 AS.reconfigure ASconfig
@02:26:52.19 AS.seek 1ml
@02:27:49.98 delay 1 second
@02:27:51.00 AFV.select :holdingCoil
@02:27:51.11 delay 1 second
@02:27:52.13 ATV.dial :waste
@02:27:53.41 AS.reconfigure ASnorm
@02:27:53.47 AS.seek 0.5ml
@02:28:19.04 ATV.dial :waste2
@02:28:20.40 AS.seek 0ml
@02:28:45.95 <bkha> Session Ended
@11:27:28.82CET19-Mar-21 <MAIN> ESP2 v3.30 I2C Gateway v3.06 for core bus
@11:27:30.89 
Configuration for mer 7/20/20 brent@mbari.org
/home/esp/esp2/type/shallow/mer/configure.rb
@11:27:30.97 
Configuration for MFB3 by preston@mbari.org
/home/esp/esp2/type/mfb/MFB3/configure.rb
@11:27:35.27 Polling Can every 10:00
@11:27:36.97 CS.configure :default
@11:27:37.01 CS.hold
@11:27:37.07 CS.configure :default
@11:27:37.15 PS.configure :default
@11:27:37.23 SS.configure :default
@11:27:37.27 SS.hold
@11:27:37.31 SS.configure :default
@11:27:37.39 CC.configure :default
@11:27:37.43 CC.hold
@11:27:37.48 CC.configure :default
@11:27:37.56 PC.configure :default
@11:27:37.60 PC.hold
@11:27:37.64 PC.configure :default
@11:27:37.74 Forearm.configure :loose
@11:27:37.78 Forearm.hold
@11:27:37.82 Forearm.configure :loose
@11:27:37.90 Elbow.configure :loose
@11:27:37.94 Elbow.hold
@11:27:37.98 Elbow.configure :loose
@11:27:38.08 SC.configure :loose
@11:27:38.11 SC.hold
@11:27:38.17 SC.configure :loose
@11:27:38.25 SE.configure :default
@11:27:38.28 SE.hold
@11:27:38.33 SE.configure :default
@11:27:38.36 CH.coast
@11:27:38.41 CH.configure :default
@11:27:38.44 PH.coast
@11:27:38.48 PH.configure :default
@11:27:38.58 SPE.coast
@11:27:38.62 SPE.configure SPEconfig
@11:27:38.69 AS.configure ASconfig
@11:27:38.73 AS.hold
@11:27:38.78 AS.configure ASconfig
@11:27:38.83 PCR.off
@11:27:38.86 PCR.pidtemp= 50,0.1,0,300,25,25
@11:27:38.89 PCR.caltemp= 2.5051,0.0372,0
@11:27:38.92 All dwarf controllers running firmware version 3.75
@11:27:39.08 Cleanly exited at 02:28:45CET18-Mar-21
@11:27:39.11 Loading /home/esp/esp2/mission/GENOMAP.rb mission
@11:27:39.25 <MFBPCR> Extracting Nucleic Acid from extSrc
@11:27:39.28 SPE.hold 2:46:40,55C
@11:27:39.32 ASV.dial :SPEdelivery
@11:27:39.75 delay 20 seconds
@11:27:41.46 <mwj> Session Begun
@11:27:59.78 <MFBPCR> AFV.select :holdingCoil
@11:27:59.91 ATV.dial :air2
@11:28:00.61 AS.reconfigure ASslow1
@11:28:00.67 AS.seek 0.07ml
@11:28:15.08 delay 3 seconds
@11:28:18.11 ATV.dial :extSrc
@11:28:19.84 AS.seek 0.47ml
@11:29:23.40 <mwj> -> abort! not "primed"
mwj:1: warning: string literal in condition
@11:29:23.42 SyntaxError in mwj -- compile error
mwj:1: syntax error, unexpected kNOT
abort! not "primed"
          ^
@11:29:32.39 -> abort! "not primed"
@11:29:32.40 CmdServer::Client::Error in mwj -- Aborting Mission: not primed
@11:29:32.46 <Qmail> BadNews.email "not primed",Subject: "Mission ABORTED!"
@11:29:40.58 <MFBPCR> delay 5 seconds
@11:29:41.08 SPE.coast
@11:29:41.15 <mwj> Session Ended
@11:31:07.18CET19-Mar-21 <MAIN> ESP2 v3.30 I2C Gateway v3.06 for core bus
@11:31:09.25 
Configuration for mer 7/20/20 brent@mbari.org
/home/esp/esp2/type/shallow/mer/configure.rb
@11:31:09.33 
Configuration for MFB3 by preston@mbari.org
/home/esp/esp2/type/mfb/MFB3/configure.rb
@11:31:13.58 Polling Can every 10:00
@11:31:15.29 CS.configure :default
@11:31:15.33 CS.hold
@11:31:15.39 CS.configure :default
@11:31:15.47 PS.configure :default
@11:31:15.55 SS.configure :default
@11:31:15.59 SS.hold
@11:31:15.64 SS.configure :default
@11:31:15.72 CC.configure :default
@11:31:15.76 CC.hold
@11:31:15.83 CC.configure :default
@11:31:15.91 PC.configure :default
@11:31:15.95 PC.hold
@11:31:15.99 PC.configure :default
@11:31:16.09 Forearm.configure :loose
@11:31:16.12 Forearm.hold
@11:31:16.17 Forearm.configure :loose
@11:31:16.25 Elbow.configure :loose
@11:31:16.29 Elbow.hold
@11:31:16.33 Elbow.configure :loose
@11:31:16.42 SC.configure :loose
@11:31:16.46 SC.hold
@11:31:16.52 SC.configure :loose
@11:31:16.60 SE.configure :default
@11:31:16.64 SE.hold
@11:31:16.69 SE.configure :default
@11:31:16.73 CH.coast
@11:31:16.77 CH.configure :default
@11:31:16.80 PH.coast
@11:31:16.85 PH.configure :default
@11:31:16.95 SPE.coast
@11:31:16.99 SPE.configure SPEconfig
@11:31:17.07 AS.configure ASconfig
@11:31:17.10 AS.hold
@11:31:17.16 AS.configure ASconfig
@11:31:17.21 PCR.off
@11:31:17.24 PCR.pidtemp= 50,0.1,0,300,25,25
@11:31:17.27 PCR.caltemp= 2.5051,0.0372,0
@11:31:17.30 All dwarf controllers running firmware version 3.75
@11:31:17.47 Cleanly exited at 11:30:59CET19-Mar-21
@11:31:17.50 Loading /home/esp/esp2/mission/GENOMAP.rb mission
@11:31:17.64 <MFBPCR> Extracting Nucleic Acid from extSrc
@11:31:17.67 SPE.hold 2:46:40,55C
@11:31:17.71 ASV.dial :SPEdelivery
@11:31:17.80 delay 20 seconds
@11:31:37.83 AFV.select :holdingCoil
@11:31:37.94 ATV.dial :air2
@11:31:39.22 AS.reconfigure ASslow1
@11:31:39.26 AS.seek 0.07ml
@11:31:41.12 <mwj> Session Begun
@11:31:53.66 <MFBPCR> delay 3 seconds
@11:31:56.69 ATV.dial :extSrc
@11:31:58.20 AS.seek 0.47ml
@11:33:18.89 delay 5 seconds
@11:33:23.97 Solid Phase Extraction Heater at 50.4C
@11:33:23.98 ATV.dial ASV
@11:33:24.83 ASV.dial :SPEdelivery
@11:33:24.92 AS.seek 0.042ml
@11:34:51.28 delay 10 seconds
@11:35:01.32 Rinsing external lysate line extSrc
@11:35:01.33 AFV.select :carrierSolution
@11:35:01.45 delay 2 seconds
@11:35:03.47 AS.reconfigure ASconfig
@11:35:03.53 AS.seek 1ml
@11:35:58.88 delay 1 second
@11:35:59.90 AFV.select :holdingCoil
@11:36:00.01 delay 1 second
@11:36:01.03 ATV.dial :extSrc
@11:36:01.87 AS.reconfigure ASnorm
@11:36:01.93 AS.seek 0ml
@11:36:52.60 ATV.dial :air2
@11:36:54.20 AS.reconfigure ASslow0
@11:36:54.26 AS.seek 0.05ml
@11:36:59.66 ATV.dial :extSrc
@11:37:01.25 AS.seek 0.01ml
@11:37:05.58 ATV.dial :waste
@11:37:06.97 AS.reconfigure ASconfig
@11:37:07.02 AS.seek 0ml
@11:37:07.89 ethanol wash
@11:37:08.23 ATV.dial :air2
@11:37:09.47 AS.reconfigure ASslow2
@11:37:09.53 AS.seek 0.01ml
@11:37:19.39 <mwj> -> exit
@11:37:19.40 Session Ended
@11:37:20.16 <MFBPCR> delay 3 seconds
@11:37:23.19 Pulling 0.4ml of Ethanol Wash
@11:37:23.20 ATV.dial :ethanol
@11:37:24.08 AS.reconfigure ASslow1
@11:37:24.12 AS.seek 0.41ml
@11:38:44.89 delay 3 seconds
@11:38:47.92 ATV.dial :air1
@11:38:48.33 delay 3 seconds
@11:38:51.40 AS.seek 0.435ml
@11:38:56.79 delay 3 seconds
@11:38:59.82 ATV.dial :ethanol
@11:39:00.45 AS.seek 0.42ml
@11:39:03.75 delay 3 seconds
@11:39:06.78 ATV.dial ASV
@11:39:08.17 AS.seek 0.093ml
@11:40:03.39 <mwj> Session Begun
@11:40:10.00 -> showlog 10
@11:40:14.19 <MFBPCR> delay 3 seconds
@11:40:17.22 ATV.dial :waste2
@11:40:18.03 AS.reconfigure ASconfig
@11:40:18.08 AS.seek 0ml
@11:40:23.74 ATV.dial :air2
@11:40:24.17 AS.reconfigure ASslow1
@11:40:24.23 AS.seek 1ml
@11:43:45.61 delay 5 seconds
@11:43:50.64 ATV.dial ASV
@11:43:51.67 ASV.dial :SPEdelivery
@11:43:51.70 AS.seek 0.02ml
@11:47:09.11 delay 5 seconds
@11:47:14.14 ATV.dial :air2
@11:47:15.23 AS.seek 0.52ml
@11:48:56.08 delay 5 seconds
@11:49:01.11 ATV.dial ASV
@11:49:02.14 ASV.dial :wasteR
@11:49:02.53 AS.seek 0.3ml
@11:49:47.03 delay 20 seconds
@11:50:07.07 AS.seek 0.07ml
@11:50:53.58 delay 20 seconds
@11:51:13.61 ATV.dial :waste2
@11:51:14.43 AS.reconfigure ASconfig
@11:51:14.49 AS.seek 0ml
@11:51:18.82 Elution
@11:51:18.83 ATV.dial :air1
@11:51:20.02 AS.reconfigure ASslow1
@11:51:20.08 AS.seek 0.05ml
@11:51:30.53 delay 3 seconds
@11:51:33.56 ATV.dial :ElutionWater
@11:51:34.30 AS.seek 0.11ml
@11:51:46.69 delay 3 seconds
@11:51:49.72 ATV.dial ASV,via: :air2
@11:51:51.95 ASV.dial :SPEelution
@11:51:52.56 AFV.select :carrierSolution
@11:51:52.72 AS.reconfigure ASnorm
@11:51:52.78 AS.seek 0.76ml
@11:52:25.88 delay 3 seconds
@11:52:28.90 AFV.select :holdingCoil
@11:52:29.05 AS.reconfigure ASslow1
@11:52:29.11 AS.seek 0.475ml
@11:53:26.72 delay 5 seconds
@11:53:31.80 AS.reconfigure ASslow2
@11:53:31.86 AS.seek 0.44ml
@11:54:07.25 delay 1:00
@11:55:07.29 pushing eluate to holding coil
@11:55:07.36 AS.seek 0.28ml
@11:57:48.56 delay 30 seconds
@11:58:18.60 ATV.dial :waste,via: :air3
@11:58:20.43 AS.reconfigure ASconfig
@11:58:20.48 AS.seek 0ml
@11:58:36.86 Parking Bleach in LysateSource line and SPEcolumn
@11:58:36.87 ATV.dial :air3
@11:58:37.78 AS.reconfigure ASslow1
@11:58:37.84 AS.seek 0.03ml
@11:58:44.16 ATV.dial :ElutionWater
@11:58:44.81 AS.seek 0.06ml
@11:58:51.10 ATV.dial :air3
@11:58:51.75 AS.seek 0.07ml
@11:58:54.13 delay 2 seconds
@11:58:56.16 ATV.dial :bleach
@11:58:56.66 AS.seek 0.15ml
@11:59:13.11 delay 3 seconds
@11:59:16.14 ATV.dial :air3
@11:59:16.72 delay 3 seconds
@11:59:19.79 AS.seek 0.175ml
@11:59:25.10 delay 3 seconds
@11:59:28.13 ATV.dial :bleach
@11:59:28.77 AS.seek 0.16ml
@11:59:32.02 delay 3 seconds
@11:59:35.05 ATV.dial :extSrc
@11:59:35.70 AS.reconfigure ASconfig
@11:59:35.75 AS.seek 0.015ml
@11:59:44.41 ATV.dial :waste
@11:59:45.97 AS.seek 0ml
@11:59:47.16 ATV.dial :air3
@11:59:48.05 AS.reconfigure ASslow1
@11:59:48.11 AS.seek 0.01ml
@11:59:50.42 delay 2 seconds
@11:59:52.45 ATV.dial :bleach
@11:59:52.92 AS.seek 0.58ml
@12:00:56.42 <mwj> Session Ended
@12:01:47.86 <MFBPCR> delay 3 seconds
@12:01:50.89 ATV.dial :air3
@12:01:51.46 delay 3 seconds
@12:01:54.53 AS.seek 0.605ml
@12:01:59.90 delay 3 seconds
@12:02:02.93 ATV.dial :bleach
@12:02:03.56 AS.seek 0.59ml
@12:02:06.85 delay 3 seconds
@12:02:09.88 ATV.dial :ASV
@12:02:10.97 ASV.dial :wasteR
@12:02:11.59 AS.seek 0ml
@12:04:10.49 delay 1:00
@12:05:10.52 AFV.select :carrierSolution
@12:05:10.63 delay 2 seconds
@12:05:12.65 AS.reconfigure ASconfig
@12:05:12.70 AS.seek 1ml
@12:06:10.42 delay 1 second
@12:06:11.44 AFV.select :holdingCoil
@12:06:11.55 delay 1 second
@12:06:12.57 ATV.dial ASV
@12:06:12.58 ASV.dial :wasteR
@12:06:12.59 AS.reconfigure ASslow1
@12:06:12.64 AS.seek 0ml
@12:09:33.99 delay 20 seconds
@12:09:54.03 ATV.dial :air3
@12:09:55.64 AS.seek 0.59ml
@12:11:54.56 delay 3 seconds
@12:11:57.59 ATV.dial :ASV
@12:11:59.10 ASV.dial :wasteR
@12:11:59.17 AS.seek 0.09ml
@12:13:40.03 delay 30 seconds
@12:14:10.07 ATV.dial :extSrc
@12:14:10.88 delay 3 seconds
@12:14:13.91 ATV.dial :ElutionWater
@12:14:15.10 AS.seek 0.12ml
@12:14:21.41 ATV.dial :air3
@12:14:22.05 AS.seek 0.13ml
@12:14:24.30 delay 2 seconds
@12:14:26.33 ATV.dial :bleach
@12:14:26.81 AS.seek 0.23ml
@12:14:47.21 delay 3 seconds
@12:14:50.24 ATV.dial :air3
@12:14:50.82 delay 3 seconds
@12:14:53.89 AS.seek 0.255ml
@12:14:59.24 delay 3 seconds
@12:15:02.27 ATV.dial :bleach
@12:15:02.89 AS.seek 0.24ml
@12:15:06.64 delay 3 seconds
@12:15:09.67 ATV.dial :ASV
@12:15:10.78 ASV.dial :SPEdelivery
@12:15:11.17 AFV.select :carrierSolution
@12:15:11.28 delay 2 seconds
@12:15:13.30 AS.reconfigure ASconfig
@12:15:13.35 AS.seek 0.5ml
@12:15:28.57 delay 1 second
@12:15:29.59 AFV.select :holdingCoil
@12:15:29.74 AS.reconfigure ASslow1
@12:15:29.79 AS.seek 0.17ml
@12:16:36.42 SPE.hold 2:46:40,95C
@12:16:36.47 delay 3:00
@12:18:39.28 <mwj> Session Begun
@12:18:43.95 -> showlog 10
@12:18:51.91 -> showlog 40
@12:19:36.50 <MFBPCR> AFV.select :carrierSolution
@12:19:36.62 delay 2 seconds
@12:19:38.64 AS.reconfigure ASconfig
@12:19:38.71 AS.seek 1ml
@12:20:26.71 delay 1 second
@12:20:27.73 AFV.select :holdingCoil
@12:20:27.84 delay 1 second
@12:20:28.86 ASV.dial :wasteD
@12:20:29.98 AS.seek 0ml
@12:21:27.75 Priming Eluate
@12:21:27.76 AFV.select :carrierSolution
@12:21:27.89 delay 2 seconds
@12:21:29.91 AS.seek 0.5ml
@12:21:58.96 delay 1 second
@12:21:59.98 AFV.select :holdingCoil
@12:22:00.09 ATV.dial ASV
@12:22:00.10 ASV.dial :SPErecovery
@12:22:00.74 Swish #1 of 2
@12:22:00.83 AS.reconfigure ASslow0
@12:22:00.88 AS.seek 0.6ml
@12:22:11.34 AS.seek 0.5ml
@12:22:21.69 Swish #2 of 2
@12:22:21.78 AS.seek 0.6ml
@12:22:32.27 AS.seek 0.5ml
@12:22:42.67 delay 3 seconds
@12:22:45.74 AS.reconfigure ASslow1
@12:22:45.80 AS.seek 0.684ml
@12:23:23.07 delay 3 seconds
@12:23:26.10 Eluate primed
@12:23:26.11 ATV.dial :waste2
@12:23:26.79 AS.reconfigure ASnorm
@12:23:26.85 AS.seek 0ml
@12:24:01.63 SPE.coast
@12:24:01.72 ASV.dial :SPErecovery
@12:24:01.74 Setting up PCR for ACYPP2 -- writing /var/log/esp/ACYPP221mar1912h.pcr
@12:24:01.76 Building PCR Slug from ASV with MM1 mix, PP2 primer
@12:24:01.79 Priming with carrier
@12:24:01.81 ATV.dial ARV
@12:24:02.86 ARV.dial :carrier
@12:24:03.22 AS.seek 0.5ml
@12:24:28.80 AFV.select :carrierSolution
@12:24:28.93 delay 2 seconds
@12:24:30.95 AS.reconfigure ASconfig
@12:24:31.01 AS.seek 1ml
@12:25:00.03 delay 1 second
@12:25:01.05 AFV.select :holdingCoil
@12:25:01.16 delay 1 second
@12:25:02.18 ATV.dial :waste
@12:25:03.81 AS.reconfigure ASnorm
@12:25:03.87 AS.seek 0ml
@12:25:54.55 AFV.select :carrierSolution
@12:25:54.68 delay 2 seconds
@12:25:56.71 AS.reconfigure ASconfig
@12:25:56.77 AS.seek 0.5ml
@12:26:25.85 delay 1 second
@12:26:26.87 AFV.select :holdingCoil
@12:26:26.98 Building Slug
@12:26:26.99 ATV.dial :ARV
@12:26:28.77 delay 2 seconds
@12:26:30.80 ARV.dial :air1
@12:26:31.19 delay 2 seconds
@12:26:33.26 AS.reconfigure ASslow2
@12:26:33.32 AS.seek 0.506ml
@12:26:39.97 delay 2 seconds
@12:26:42.00 ARV.dial :MM1
@12:26:42.63 delay 2 seconds
@12:26:44.66 Adding MM1
@12:26:44.74 AS.seek 0.524ml
@12:27:03.00 delay 25 seconds
@12:27:28.03 ARV.dial :PP2
@12:27:28.45 Adding PP2
@12:27:28.46 delay 2 seconds
@12:27:30.54 AS.seek 0.53ml
@12:27:36.92 delay 10 seconds
@12:27:46.96 ARV.dial :air2
@12:27:47.37 adding ARV air
@12:27:47.38 delay 2 seconds
@12:27:49.46 AS.seek 0.536ml
@12:27:55.71 delay 5 seconds
@12:28:00.74 ARV.dial :carrier
@12:28:02.08 delay 7 seconds
@12:28:09.16 AS.seek 0.594ml
@12:29:07.61 delay 5 seconds
@12:29:12.64 ATV.dial :ASV
@12:29:13.05 delay 2 seconds
@12:29:15.13 AS.seek 0.6ml
@12:29:21.39 delay 4 seconds
@12:29:25.42 ATV.dial ARV
@12:29:25.99 delay 2 seconds
@12:29:28.02 ARV.dial :carrier
@12:29:28.03 delay 2 seconds
@12:29:30.11 AS.seek 0.65ml
@12:30:20.88 delay 4 seconds
@12:30:24.91 ATV.dial PCR
@12:30:25.57 ASV.dial :waste1
@12:30:26.38 ARV.dial :air1
@12:30:26.81 Positioning Slug by 0.324ml
@12:30:26.89 AS.seek 0.326ml
@12:35:53.10 delay 5 seconds
@12:35:58.13 ATV.dial ARV
@12:35:58.94 ASV.dial :PCRlock
@12:35:59.33 delay 5 seconds
@12:36:04.36 Running ACYPP2 Profile60CIQ2 thermocycling
@12:36:04.37 <PCR> PCR.program Profile60CIQ2
@12:36:04.50 Profile60CIQ2 should complete in < 1:47:50
@12:36:04.51 PCR.run 2:19:47.5
@13:28:41.42 <mwj> -> exit
@13:28:41.43 Session Ended
@14:03:54.24 <PCR> PCR.off
@14:03:54.85 <MFBPCR> Running ACYPP2 MeltProfile60 thermocycling
@14:03:54.86 <PCR> PCR.program MeltProfile60
@14:03:54.95 MeltProfile60 should complete in < 11:55
@14:03:54.96 PCR.run 19:53.75
@14:11:11.81 PCR.off
@14:11:12.73 <MFBPCR> PCR for ACYPP2 completed -- closed /var/log/esp/ACYPP221mar1912h.pcr
@14:11:12.79 Cleaning PCR
@14:11:12.80 AFV.select :carrierSolution
@14:11:12.90 delay 2 seconds
@14:11:14.92 AS.reconfigure ASconfig
@14:11:14.97 AS.seek 0.5ml
@14:11:25.22 delay 1 second
@14:11:26.24 AFV.select :holdingCoil
@14:11:26.35 ASV.dial :waste1
@14:11:26.72 ATV.dial :air3
@14:11:28.08 AS.reconfigure ASslow1
@14:11:28.12 AS.seek 0.51ml
@14:11:30.39 delay 2 seconds
@14:11:32.41 ATV.dial :bleach
@14:11:33.03 AS.seek 0.81ml
@14:12:33.68 delay 3 seconds
@14:12:36.71 ATV.dial :air3
@14:12:37.27 delay 3 seconds
@14:12:40.34 AS.seek 0.835ml
@14:12:45.64 delay 3 seconds
@14:12:48.67 ATV.dial :bleach
@14:12:49.30 AS.seek 0.82ml
@14:12:52.60 delay 3 seconds
@14:12:55.63 ARV.dial :waste
@14:12:56.26 delay 3 seconds
@14:12:59.29 ATV.dial :ARV
@14:13:00.21 AS.reconfigure ASconfig
@14:13:00.25 AS.seek 0.47ml
@14:13:20.65 AFV.select :carrierSolution
@14:13:20.76 delay 2 seconds
@14:13:22.78 AS.seek 1ml
@14:13:53.51 delay 1 second
@14:13:54.53 AFV.select :holdingCoil
@14:13:54.64 delay 1 second
@14:13:55.66 AS.reconfigure ASfast
@14:13:55.71 AS.seek 0ml
@14:14:23.05 AFV.select :carrierSolution
@14:14:23.16 delay 2 seconds
@14:14:25.18 AS.reconfigure ASconfig
@14:14:25.23 AS.seek 1ml
@14:15:22.97 delay 1 second
@14:15:23.99 AFV.select :holdingCoil
@14:15:24.10 delay 1 second
@14:15:25.12 AS.reconfigure ASfast
@14:15:25.17 AS.seek 0ml
@14:15:52.51 AFV.select :carrierSolution
@14:15:52.62 delay 2 seconds
@14:15:54.64 AS.reconfigure ASconfig
@14:15:54.69 AS.seek 1ml
@14:16:52.41 delay 1 second
@14:16:53.43 AFV.select :holdingCoil
@14:16:53.54 delay 1 second
@14:16:54.56 AS.reconfigure ASfast
@14:16:54.61 AS.seek 0ml
@14:17:21.91 ARV.dial between carrier and air1
@14:17:22.43 ATV.dial :air3
@14:17:23.78 AS.reconfigure ASslow1
@14:17:23.82 AS.seek 0.01ml
@14:17:26.10 delay 2 seconds
@14:17:28.13 ATV.dial :bleach
@14:17:28.75 AS.seek 0.81ml
@14:20:09.86 delay 3 seconds
@14:20:12.89 ATV.dial :air3
@14:20:13.46 delay 3 seconds
@14:20:16.53 AS.seek 0.835ml
@14:20:21.84 delay 3 seconds
@14:20:24.87 ATV.dial :bleach
@14:20:25.50 AS.seek 0.82ml
@14:20:28.82 delay 3 seconds
@14:20:31.85 ATV.dial PCR
@14:20:32.27 AS.reconfigure ASconfig
@14:20:32.31 AS.seek 0ml
@14:21:19.68 PCR.program :bleachProfile
@14:21:19.75 PCR program should complete in < 2:45
@14:21:19.76 PCR.run 10:00
@14:24:18.96 PCR.off
@14:24:18.99 AFV.select :carrierSolution
@14:24:19.10 delay 2 seconds
@14:24:21.12 AS.seek 1ml
@14:25:18.88 delay 1 second
@14:25:19.90 AFV.select :holdingCoil
@14:25:20.01 delay 1 second
@14:25:21.03 AS.reconfigure ASfast
@14:25:21.08 AS.seek 0ml
@14:25:48.41 delay 3 seconds
@14:25:51.43 AFV.select :carrierSolution
@14:25:51.54 delay 2 seconds
@14:25:53.56 AS.reconfigure ASconfig
@14:25:53.65 AS.seek 1ml
@14:26:51.41 delay 1 second
@14:26:52.43 AFV.select :holdingCoil
@14:26:52.54 delay 1 second
@14:26:53.56 AS.reconfigure ASfast
@14:26:53.61 AS.seek 0ml
@14:27:20.94 delay 3 seconds
@14:27:23.96 AFV.select :carrierSolution
@14:27:24.07 delay 2 seconds
@14:27:26.09 AS.reconfigure ASconfig
@14:27:26.14 AS.seek 1ml
@14:28:23.88 delay 1 second
@14:28:24.90 AFV.select :holdingCoil
@14:28:25.01 delay 1 second
@14:28:26.03 AS.reconfigure ASfast
@14:28:26.08 AS.seek 0ml
@14:28:53.40 delay 3 seconds
@14:28:56.42 AFV.select :carrierSolution
@14:28:56.53 delay 2 seconds
@14:28:58.55 AS.reconfigure ASconfig
@14:28:58.60 AS.seek 1ml
@14:29:56.33 delay 1 second
@14:29:57.35 AFV.select :holdingCoil
@14:29:57.46 delay 1 second
@14:29:58.48 AS.reconfigure ASfast
@14:29:58.53 AS.seek 0ml
@14:30:25.83 delay 3 seconds
@14:30:28.85 AFV.select :carrierSolution
@14:30:28.96 delay 2 seconds
@14:30:30.98 AS.reconfigure ASconfig
@14:30:31.04 AS.seek 1ml
@14:31:28.82 delay 1 second
@14:31:29.84 AFV.select :holdingCoil
@14:31:29.95 delay 1 second
@14:31:30.97 AS.reconfigure ASnorm
@14:31:31.02 AS.seek 0ml
@14:32:21.72 Setting up PCR for AOPP1 -- writing /var/log/esp/AOPP121mar1914h.pcr
@14:32:21.73 Building PCR Slug from ASV with MM1 mix, PP1 primer
@14:32:21.74 Priming with carrier
@14:32:21.75 ATV.dial ARV
@14:32:22.38 ARV.dial :carrier
@14:32:22.72 AS.seek 0.5ml
@14:32:48.26 AFV.select :carrierSolution
@14:32:48.37 delay 2 seconds
@14:32:50.39 AS.reconfigure ASconfig
@14:32:50.44 AS.seek 1ml
@14:33:19.43 delay 1 second
@14:33:20.45 AFV.select :holdingCoil
@14:33:20.56 delay 1 second
@14:33:21.58 ATV.dial :waste
@14:33:23.34 AS.reconfigure ASnorm
@14:33:23.38 AS.seek 0ml
@14:34:14.12 AFV.select :carrierSolution
@14:34:14.23 delay 2 seconds
@14:34:16.25 AS.reconfigure ASconfig
@14:34:16.30 AS.seek 0.5ml
@14:34:45.32 delay 1 second
@14:34:46.34 AFV.select :holdingCoil
@14:34:46.45 Building Slug
@14:34:46.46 ATV.dial :ARV
@14:34:48.19 delay 2 seconds
@14:34:50.22 ARV.dial :air1
@14:34:50.62 delay 2 seconds
@14:34:52.68 AS.reconfigure ASslow2
@14:34:52.73 AS.seek 0.506ml
@14:34:59.05 delay 2 seconds
@14:35:01.08 ARV.dial :MM1
@14:35:01.69 delay 2 seconds
@14:35:03.72 Adding MM1
@14:35:03.78 AS.seek 0.524ml
@14:35:22.37 delay 25 seconds
@14:35:47.40 ARV.dial :PP1
@14:35:47.78 Adding PP1
@14:35:47.79 delay 2 seconds
@14:35:49.85 AS.seek 0.53ml
@14:35:56.02 delay 10 seconds
@14:36:06.06 ARV.dial :air1
@14:36:06.47 adding ARV air
@14:36:06.48 delay 2 seconds
@14:36:08.55 AS.seek 0.536ml
@14:36:14.88 delay 5 seconds
@14:36:19.91 ARV.dial :carrier
@14:36:20.32 delay 7 seconds
@14:36:27.40 AS.seek 0.594ml
@14:37:26.21 delay 5 seconds
@14:37:31.24 ATV.dial :ASV
@14:37:31.65 delay 2 seconds
@14:37:33.72 AS.seek 0.6ml
@14:37:40.16 delay 4 seconds
@14:37:44.19 ATV.dial ARV
@14:37:44.76 delay 2 seconds
@14:37:46.79 ARV.dial :carrier
@14:37:46.80 delay 2 seconds
@14:37:48.86 AS.seek 0.65ml
@14:38:39.68 delay 4 seconds
@14:38:43.71 ATV.dial PCR
@14:38:44.33 ASV.dial :waste1
@14:38:44.35 ARV.dial :air1
@14:38:44.75 Positioning Slug by 0.324ml
@14:38:44.82 AS.seek 0.326ml
@14:44:10.58 delay 5 seconds
@14:44:15.61 ATV.dial ARV
@14:44:16.40 ASV.dial :PCRlock
@14:44:16.79 delay 5 seconds
@14:44:21.82 Running AOPP1 Profile60CIQ1 thermocycling
@14:44:21.83 <PCR> PCR.program Profile60CIQ1
@14:44:21.93 Profile60CIQ1 should complete in < 1:56:15
@14:44:21.94 PCR.run 2:30:18.75
@14:49:50.91 <mwj> Session Begun
@14:49:57.62 -> showlog 10
@14:50:09.34 -> exit
@14:50:09.35 Session Ended
@16:22:50.57 <PCR> PCR.off
@16:22:51.12 <MFBPCR> Running AOPP1 MeltProfile60 thermocycling
@16:22:51.13 <PCR> PCR.program MeltProfile60
@16:22:51.22 MeltProfile60 should complete in < 11:55
@16:22:51.23 PCR.run 19:53.75
@16:30:16.72 PCR.off
@16:30:17.63 <MFBPCR> PCR for AOPP1 completed -- closed /var/log/esp/AOPP121mar1914h.pcr
@16:30:17.69 Cleaning PCR
@16:30:17.70 AFV.select :carrierSolution
@16:30:17.80 delay 2 seconds
@16:30:19.82 AS.reconfigure ASconfig
@16:30:19.87 AS.seek 0.5ml
@16:30:30.17 delay 1 second
@16:30:31.19 AFV.select :holdingCoil
@16:30:31.30 ASV.dial :waste1
@16:30:31.68 ATV.dial :air3
@16:30:33.02 AS.reconfigure ASslow1
@16:30:33.44 AS.seek 0.51ml
@16:30:35.82 delay 2 seconds
@16:30:37.85 ATV.dial :bleach
@16:30:38.48 AS.seek 0.81ml
@16:31:39.07 delay 3 seconds
@16:31:42.10 ATV.dial :air3
@16:31:42.67 delay 3 seconds
@16:31:45.74 AS.seek 0.835ml
@16:31:51.04 delay 3 seconds
@16:31:54.07 ATV.dial :bleach
@16:31:54.69 AS.seek 0.82ml
@16:31:57.99 delay 3 seconds
@16:32:01.02 ARV.dial :waste
@16:32:01.63 delay 3 seconds
@16:32:04.66 ATV.dial :ARV
@16:32:05.59 AS.reconfigure ASconfig
@16:32:05.63 AS.seek 0.47ml
@16:32:26.03 AFV.select :carrierSolution
@16:32:26.14 delay 2 seconds
@16:32:28.16 AS.seek 1ml
@16:32:58.92 delay 1 second
@16:32:59.94 AFV.select :holdingCoil
@16:33:00.05 delay 1 second
@16:33:01.07 AS.reconfigure ASfast
@16:33:01.12 AS.seek 0ml
@16:33:28.46 AFV.select :carrierSolution
@16:33:28.57 delay 2 seconds
@16:33:30.59 AS.reconfigure ASconfig
@16:33:30.64 AS.seek 1ml
@16:34:28.37 delay 1 second
@16:34:29.39 AFV.select :holdingCoil
@16:34:29.50 delay 1 second
@16:34:30.52 AS.reconfigure ASfast
@16:34:30.57 AS.seek 0ml
@16:34:57.88 AFV.select :carrierSolution
@16:34:57.99 delay 2 seconds
@16:35:00.01 AS.reconfigure ASconfig
@16:35:00.06 AS.seek 1ml
@16:35:57.81 delay 1 second
@16:35:58.83 AFV.select :holdingCoil
@16:35:58.94 delay 1 second
@16:35:59.96 AS.reconfigure ASfast
@16:36:00.01 AS.seek 0ml
@16:36:27.35 ARV.dial between carrier and air1
@16:36:27.85 ATV.dial :air3
@16:36:29.21 AS.reconfigure ASslow1
@16:36:29.25 AS.seek 0.01ml
@16:36:31.57 delay 2 seconds
@16:36:33.60 ATV.dial :bleach
@16:36:34.23 AS.seek 0.81ml
@16:39:15.37 delay 3 seconds
@16:39:18.40 ATV.dial :air3
@16:39:18.96 delay 3 seconds
@16:39:22.03 AS.seek 0.835ml
@16:39:27.31 delay 3 seconds
@16:39:30.34 ATV.dial :bleach
@16:39:30.96 AS.seek 0.82ml
@16:39:34.24 delay 3 seconds
@16:39:37.27 ATV.dial PCR
@16:39:37.69 AS.reconfigure ASconfig
@16:39:37.73 AS.seek 0ml
@16:40:25.13 PCR.program :bleachProfile
@16:40:25.19 PCR program should complete in < 2:45
@16:40:25.20 PCR.run 10:00
@16:43:21.39 PCR.off
@16:43:21.42 AFV.select :carrierSolution
@16:43:21.53 delay 2 seconds
@16:43:23.55 AS.seek 1ml
@16:44:21.29 delay 1 second
@16:44:22.31 AFV.select :holdingCoil
@16:44:22.42 delay 1 second
@16:44:23.44 AS.reconfigure ASfast
@16:44:23.49 AS.seek 0ml
@16:44:50.82 delay 3 seconds
@16:44:53.84 AFV.select :carrierSolution
@16:44:53.95 delay 2 seconds
@16:44:55.97 AS.reconfigure ASconfig
@16:44:56.02 AS.seek 1ml
@16:45:53.76 delay 1 second
@16:45:54.78 AFV.select :holdingCoil
@16:45:54.89 delay 1 second
@16:45:55.91 AS.reconfigure ASfast
@16:45:55.96 AS.seek 0ml
@16:46:23.29 delay 3 seconds
@16:46:26.31 AFV.select :carrierSolution
@16:46:26.42 delay 2 seconds
@16:46:28.44 AS.reconfigure ASconfig
@16:46:28.50 AS.seek 1ml
@16:47:26.24 delay 1 second
@16:47:27.26 AFV.select :holdingCoil
@16:47:27.37 delay 1 second
@16:47:28.39 AS.reconfigure ASfast
@16:47:28.44 AS.seek 0ml
@16:47:55.78 delay 3 seconds
@16:47:58.80 AFV.select :carrierSolution
@16:47:58.91 delay 2 seconds
@16:48:00.93 AS.reconfigure ASconfig
@16:48:00.99 AS.seek 1ml
@16:48:58.73 delay 1 second
@16:48:59.75 AFV.select :holdingCoil
@16:48:59.86 delay 1 second
@16:49:00.88 AS.reconfigure ASfast
@16:49:00.93 AS.seek 0ml
@16:49:28.23 delay 3 seconds
@16:49:31.25 AFV.select :carrierSolution
@16:49:31.36 delay 2 seconds
@16:49:33.38 AS.reconfigure ASconfig
@16:49:33.43 AS.seek 1ml
@16:50:31.21 delay 1 second
@16:50:32.23 AFV.select :holdingCoil
@16:50:32.34 delay 1 second
@16:50:33.36 AS.reconfigure ASnorm
@16:50:33.41 AS.seek 0ml
@16:51:24.15 Setting up PCR for AARPP4 -- writing /var/log/esp/AARPP421mar1916h.pcr
@16:51:24.16 Building PCR Slug from ASV with MM2 mix, PP4 primer
@16:51:24.17 Priming with carrier
@16:51:24.18 ATV.dial ARV
@16:51:24.82 ARV.dial :carrier
@16:51:25.16 AS.seek 0.5ml
@16:51:50.71 AFV.select :carrierSolution
@16:51:50.82 delay 2 seconds
@16:51:52.84 AS.reconfigure ASconfig
@16:51:52.89 AS.seek 1ml
@16:52:21.90 delay 1 second
@16:52:22.92 AFV.select :holdingCoil
@16:52:23.03 delay 1 second
@16:52:24.05 ATV.dial :waste
@16:52:25.82 AS.reconfigure ASnorm
@16:52:25.86 AS.seek 0ml
@16:53:16.54 AFV.select :carrierSolution
@16:53:16.65 delay 2 seconds
@16:53:18.67 AS.reconfigure ASconfig
@16:53:18.72 AS.seek 0.5ml
@16:53:47.73 delay 1 second
@16:53:48.75 AFV.select :holdingCoil
@16:53:48.86 Building Slug
@16:53:48.87 ATV.dial :ARV
@16:53:50.60 delay 2 seconds
@16:53:52.63 ARV.dial :air1
@16:53:53.02 delay 2 seconds
@16:53:55.09 AS.reconfigure ASslow2
@16:53:55.14 AS.seek 0.506ml
@16:54:01.79 delay 2 seconds
@16:54:03.82 ARV.dial :MM2
@16:54:05.37 delay 2 seconds
@16:54:07.40 Adding MM2
@16:54:07.46 AS.seek 0.524ml
@16:54:25.81 delay 25 seconds
@16:54:50.84 ARV.dial :PP4
@16:54:51.24 Adding PP4
@16:54:51.25 delay 2 seconds
@16:54:53.31 AS.seek 0.53ml
@16:55:00.04 delay 10 seconds
@16:55:10.08 ARV.dial :air3
@16:55:10.47 adding ARV air
@16:55:10.48 delay 2 seconds
@16:55:12.54 AS.seek 0.536ml
@16:55:18.78 delay 5 seconds
@16:55:23.81 ARV.dial :carrier
@16:55:25.10 delay 7 seconds
@16:55:32.17 AS.seek 0.594ml
@16:56:30.59 delay 5 seconds
@16:56:35.62 ATV.dial :ASV
@16:56:36.04 delay 2 seconds
@16:56:38.11 AS.seek 0.6ml
@16:56:44.31 delay 4 seconds
@16:56:48.34 ATV.dial ARV
@16:56:48.90 delay 2 seconds
@16:56:50.93 ARV.dial :carrier
@16:56:50.94 delay 2 seconds
@16:56:53.00 AS.seek 0.65ml
@16:57:43.74 delay 4 seconds
@16:57:47.77 ATV.dial PCR
@16:57:48.40 ASV.dial :waste1
@16:57:48.42 ARV.dial :air1
@16:57:48.82 Positioning Slug by 0.324ml
@16:57:48.89 AS.seek 0.326ml
@17:03:14.89 delay 5 seconds
@17:03:19.92 ATV.dial ARV
@17:03:20.71 ASV.dial :PCRlock
@17:03:21.10 delay 5 seconds
@17:03:26.13 Running AARPP4 Profile58CIQ2 thermocycling
@17:03:26.14 <PCR> PCR.program Profile58CIQ2
@17:03:26.66 Profile58CIQ2 should complete in < 1:36:15
@17:03:26.67 PCR.run 2:05:18.75
@18:23:41.07 PCR.off
@18:23:41.62 <MFBPCR> Running AARPP4 MeltProfile58 thermocycling
@18:23:41.63 <PCR> PCR.program MeltProfile58
@18:23:41.72 MeltProfile58 should complete in < 12:33
@18:23:41.73 PCR.run 20:41.25
@18:31:17.49 PCR.off
@18:31:18.83 <MFBPCR> PCR for AARPP4 completed -- closed /var/log/esp/AARPP421mar1916h.pcr
@18:31:18.89 Cleaning PCR
@18:31:18.90 AFV.select :carrierSolution
@18:31:19.00 delay 2 seconds
@18:31:21.02 AS.reconfigure ASconfig
@18:31:21.07 AS.seek 0.5ml
@18:31:31.36 delay 1 second
@18:31:32.38 AFV.select :holdingCoil
@18:31:32.49 ASV.dial :waste1
@18:31:32.87 ATV.dial :air3
@18:31:34.21 AS.reconfigure ASslow1
@18:31:34.25 AS.seek 0.51ml
@18:31:36.52 delay 2 seconds
@18:31:38.55 ATV.dial :bleach
@18:31:39.18 AS.seek 0.81ml
@18:32:39.75 delay 3 seconds
@18:32:42.78 ATV.dial :air3
@18:32:43.34 delay 3 seconds
@18:32:46.41 AS.seek 0.835ml
@18:32:51.69 delay 3 seconds
@18:32:54.72 ATV.dial :bleach
@18:32:55.35 AS.seek 0.82ml
@18:32:58.65 delay 3 seconds
@18:33:01.68 ARV.dial :waste
@18:33:02.29 delay 3 seconds
@18:33:05.32 ATV.dial :ARV
@18:33:06.24 AS.reconfigure ASconfig
@18:33:06.28 AS.seek 0.47ml
@18:33:26.68 AFV.select :carrierSolution
@18:33:26.79 delay 2 seconds
@18:33:28.81 AS.seek 1ml
@18:33:59.56 delay 1 second
@18:34:00.58 AFV.select :holdingCoil
@18:34:00.69 delay 1 second
@18:34:01.71 AS.reconfigure ASfast
@18:34:01.76 AS.seek 0ml
@18:34:29.09 AFV.select :carrierSolution
@18:34:29.20 delay 2 seconds
@18:34:31.22 AS.reconfigure ASconfig
@18:34:31.27 AS.seek 1ml
@18:35:29.01 delay 1 second
@18:35:30.03 AFV.select :holdingCoil
@18:35:30.14 delay 1 second
@18:35:31.16 AS.reconfigure ASfast
@18:35:31.21 AS.seek 0ml
@18:35:58.53 AFV.select :carrierSolution
@18:35:58.64 delay 2 seconds
@18:36:00.66 AS.reconfigure ASconfig
@18:36:00.71 AS.seek 1ml
@18:36:58.45 delay 1 second
@18:36:59.47 AFV.select :holdingCoil
@18:36:59.58 delay 1 second
@18:37:00.60 AS.reconfigure ASfast
@18:37:00.65 AS.seek 0ml
@18:37:27.98 ARV.dial between carrier and air1
@18:37:28.49 ATV.dial :air3
@18:37:29.82 AS.reconfigure ASslow1
@18:37:29.86 AS.seek 0.01ml
@18:37:32.15 delay 2 seconds
@18:37:34.18 ATV.dial :bleach
@18:37:34.79 AS.seek 0.81ml
@18:40:16.01 delay 3 seconds
@18:40:19.04 ATV.dial :air3
@18:40:19.61 delay 3 seconds
@18:40:22.68 AS.seek 0.835ml
@18:40:27.98 delay 3 seconds
@18:40:31.01 ATV.dial :bleach
@18:40:31.64 AS.seek 0.82ml
@18:40:34.95 delay 3 seconds
@18:40:37.98 ATV.dial PCR
@18:40:38.40 AS.reconfigure ASconfig
@18:40:38.44 AS.seek 0ml
@18:41:25.86 PCR.program :bleachProfile
@18:41:25.92 PCR program should complete in < 2:45
@18:41:25.93 PCR.run 10:00
@18:44:22.11 PCR.off
@18:44:22.13 AFV.select :carrierSolution
@18:44:22.24 delay 2 seconds
@18:44:24.26 AS.seek 1ml
@18:45:22.00 delay 1 second
@18:45:23.02 AFV.select :holdingCoil
@18:45:23.13 delay 1 second
@18:45:24.15 AS.reconfigure ASfast
@18:45:24.20 AS.seek 0ml
@18:45:51.50 delay 3 seconds
@18:45:54.52 AFV.select :carrierSolution
@18:45:54.63 delay 2 seconds
@18:45:56.65 AS.reconfigure ASconfig
@18:45:56.70 AS.seek 1ml
@18:46:54.48 delay 1 second
@18:46:55.50 AFV.select :holdingCoil
@18:46:55.61 delay 1 second
@18:46:56.63 AS.reconfigure ASfast
@18:46:56.68 AS.seek 0ml
@18:47:24.00 delay 3 seconds
@18:47:27.02 AFV.select :carrierSolution
@18:47:27.13 delay 2 seconds
@18:47:29.15 AS.reconfigure ASconfig
@18:47:29.20 AS.seek 1ml
@18:48:26.98 delay 1 second
@18:48:28.00 AFV.select :holdingCoil
@18:48:28.11 delay 1 second
@18:48:29.13 AS.reconfigure ASfast
@18:48:29.18 AS.seek 0ml
@18:48:56.48 delay 3 seconds
@18:48:59.50 AFV.select :carrierSolution
@18:48:59.61 delay 2 seconds
@18:49:01.63 AS.reconfigure ASconfig
@18:49:01.68 AS.seek 1ml
@18:49:59.42 delay 1 second
@18:50:00.44 AFV.select :holdingCoil
@18:50:00.55 delay 1 second
@18:50:01.57 AS.reconfigure ASfast
@18:50:01.62 AS.seek 0ml
@18:50:28.93 delay 3 seconds
@18:50:31.95 AFV.select :carrierSolution
@18:50:32.06 delay 2 seconds
@18:50:34.08 AS.reconfigure ASconfig
@18:50:34.13 AS.seek 1ml
@18:51:31.87 delay 1 second
@18:51:32.89 AFV.select :holdingCoil
@18:51:32.99 delay 1 second
@18:51:34.01 AS.reconfigure ASnorm
@18:51:34.06 AS.seek 0ml
@18:52:24.78 Setting up PCR for AALPP3 -- writing /var/log/esp/AALPP321mar1918h.pcr
@18:52:24.79 Building PCR Slug from ASV with MM2 mix, PP3 primer
@18:52:24.80 Priming with carrier
@18:52:24.81 ATV.dial ARV
@18:52:25.45 ARV.dial :carrier
@18:52:25.78 AS.seek 0.5ml
@18:52:51.31 AFV.select :carrierSolution
@18:52:51.42 delay 2 seconds
@18:52:53.44 AS.reconfigure ASconfig
@18:52:53.49 AS.seek 1ml
@18:53:22.51 delay 1 second
@18:53:23.54 AFV.select :holdingCoil
@18:53:23.64 delay 1 second
@18:53:24.66 ATV.dial :waste
@18:53:26.43 AS.reconfigure ASnorm
@18:53:26.47 AS.seek 0ml
@18:54:17.14 AFV.select :carrierSolution
@18:54:17.25 delay 2 seconds
@18:54:19.27 AS.reconfigure ASconfig
@18:54:19.32 AS.seek 0.5ml
@18:54:48.33 delay 1 second
@18:54:49.35 AFV.select :holdingCoil
@18:54:49.46 Building Slug
@18:54:49.47 ATV.dial :ARV
@18:54:51.20 delay 2 seconds
@18:54:53.23 ARV.dial :air1
@18:54:53.62 delay 2 seconds
@18:54:55.69 AS.reconfigure ASslow2
@18:54:55.73 AS.seek 0.506ml
@18:55:02.08 delay 2 seconds
@18:55:04.11 ARV.dial :MM2
@18:55:05.64 delay 2 seconds
@18:55:07.67 Adding MM2
@18:55:07.73 AS.seek 0.524ml
@18:55:26.14 delay 25 seconds
@18:55:51.17 ARV.dial :PP3
@18:55:51.54 Adding PP3
@18:55:51.56 delay 2 seconds
@18:55:53.62 AS.seek 0.53ml
@18:55:59.95 delay 10 seconds
@18:56:09.99 ARV.dial :air2
@18:56:10.39 adding ARV air
@18:56:10.41 delay 2 seconds
@18:56:12.47 AS.seek 0.536ml
@18:56:18.89 delay 5 seconds
@18:56:23.92 ARV.dial :carrier
@18:56:25.23 delay 7 seconds
@18:56:32.30 AS.seek 0.594ml
@18:57:30.89 delay 5 seconds
@18:57:35.92 ATV.dial :ASV
@18:57:36.34 delay 2 seconds
@18:57:38.82 AS.seek 0.6ml
@18:57:45.01 delay 4 seconds
@18:57:49.04 ATV.dial ARV
@18:57:49.61 delay 2 seconds
@18:57:51.64 ARV.dial :carrier
@18:57:51.65 delay 2 seconds
@18:57:53.71 AS.seek 0.65ml
@18:58:44.51 delay 4 seconds
@18:58:48.54 ATV.dial PCR
@18:58:49.17 ASV.dial :waste1
@18:58:49.19 ARV.dial :air1
@18:58:49.59 Positioning Slug by 0.324ml
@18:58:49.66 AS.seek 0.326ml
@19:04:15.69 delay 5 seconds
@19:04:20.72 ATV.dial ARV
@19:04:21.51 ASV.dial :PCRlock
@19:04:21.90 delay 5 seconds
@19:04:26.93 Running AALPP3 Profile58CIQ2 thermocycling
@19:04:26.94 <PCR> PCR.program Profile58CIQ2
@19:04:27.04 Profile58CIQ2 should complete in < 1:36:15
@19:04:27.05 PCR.run 2:05:18.75
@20:24:29.62 PCR.off
@20:24:30.17 <MFBPCR> Running AALPP3 MeltProfile58 thermocycling
@20:24:30.18 <PCR> PCR.program MeltProfile58
@20:24:30.63 MeltProfile58 should complete in < 12:33
@20:24:30.65 PCR.run 20:41.25
@20:31:59.87 PCR.off
@20:32:00.83 <MFBPCR> PCR for AALPP3 completed -- closed /var/log/esp/AALPP321mar1918h.pcr
@20:32:00.88 Cleaning PCR
@20:32:00.89 AFV.select :carrierSolution
@20:32:01.00 delay 2 seconds
@20:32:03.02 AS.reconfigure ASconfig
@20:32:03.07 AS.seek 0.5ml
@20:32:13.34 delay 1 second
@20:32:14.36 AFV.select :holdingCoil
@20:32:14.47 ASV.dial :waste1
@20:32:14.84 ATV.dial :air3
@20:32:16.19 AS.reconfigure ASslow1
@20:32:16.23 AS.seek 0.51ml
@20:32:18.50 delay 2 seconds
@20:32:20.52 ATV.dial :bleach
@20:32:21.14 AS.seek 0.81ml
@20:33:21.78 delay 3 seconds
@20:33:24.81 ATV.dial :air3
@20:33:25.38 delay 3 seconds
@20:33:28.45 AS.seek 0.835ml
@20:33:33.78 delay 3 seconds
@20:33:36.81 ATV.dial :bleach
@20:33:37.44 AS.seek 0.82ml
@20:33:40.74 delay 3 seconds
@20:33:43.77 ARV.dial :waste
@20:33:44.38 delay 3 seconds
@20:33:47.41 ATV.dial :ARV
@20:33:48.33 AS.reconfigure ASconfig
@20:33:48.37 AS.seek 0.47ml
@20:34:08.75 AFV.select :carrierSolution
@20:34:08.86 delay 2 seconds
@20:34:10.88 AS.seek 1ml
@20:34:41.63 delay 1 second
@20:34:42.65 AFV.select :holdingCoil
@20:34:42.76 delay 1 second
@20:34:43.78 AS.reconfigure ASfast
@20:34:43.83 AS.seek 0ml
@20:35:11.14 AFV.select :carrierSolution
@20:35:11.25 delay 2 seconds
@20:35:13.27 AS.reconfigure ASconfig
@20:35:13.32 AS.seek 1ml
@20:36:11.06 delay 1 second
@20:36:12.08 AFV.select :holdingCoil
@20:36:12.19 delay 1 second
@20:36:13.21 AS.reconfigure ASfast
@20:36:13.26 AS.seek 0ml
@20:36:40.56 AFV.select :carrierSolution
@20:36:40.67 delay 2 seconds
@20:36:42.69 AS.reconfigure ASconfig
@20:36:42.74 AS.seek 1ml
@20:37:40.49 delay 1 second
@20:37:41.51 AFV.select :holdingCoil
@20:37:41.62 delay 1 second
@20:37:42.64 AS.reconfigure ASfast
@20:37:42.69 AS.seek 0ml
@20:38:10.02 ARV.dial between carrier and air1
@20:38:10.53 ATV.dial :air3
@20:38:11.87 AS.reconfigure ASslow1
@20:38:11.91 AS.seek 0.01ml
@20:38:14.20 delay 2 seconds
@20:38:16.23 ATV.dial :bleach
@20:38:16.86 AS.seek 0.81ml
@20:40:57.99 delay 3 seconds
@20:41:01.02 ATV.dial :air3
@20:41:01.58 delay 3 seconds
@20:41:04.65 AS.seek 0.835ml
@20:41:10.05 delay 3 seconds
@20:41:13.08 ATV.dial :bleach
@20:41:13.70 AS.seek 0.82ml
@20:41:16.91 delay 3 seconds
@20:41:19.94 ATV.dial PCR
@20:41:20.36 AS.reconfigure ASconfig
@20:41:20.40 AS.seek 0ml
@20:42:07.81 PCR.program :bleachProfile
@20:42:07.87 PCR program should complete in < 2:45
@20:42:07.88 PCR.run 10:00
@20:45:07.10 PCR.off
@20:45:07.12 AFV.select :carrierSolution
@20:45:07.23 delay 2 seconds
@20:45:09.25 AS.seek 1ml
@20:46:06.99 delay 1 second
@20:46:08.01 AFV.select :holdingCoil
@20:46:08.12 delay 1 second
@20:46:09.14 AS.reconfigure ASfast
@20:46:09.19 AS.seek 0ml
@20:46:36.52 delay 3 seconds
@20:46:39.54 AFV.select :carrierSolution
@20:46:39.65 delay 2 seconds
@20:46:41.67 AS.reconfigure ASconfig
@20:46:41.72 AS.seek 1ml
@20:47:39.49 delay 1 second
@20:47:40.51 AFV.select :holdingCoil
@20:47:40.62 delay 1 second
@20:47:41.64 AS.reconfigure ASfast
@20:47:41.69 AS.seek 0ml
@20:48:09.02 delay 3 seconds
@20:48:12.04 AFV.select :carrierSolution
@20:48:12.15 delay 2 seconds
@20:48:14.17 AS.reconfigure ASconfig
@20:48:14.26 AS.seek 1ml
@20:49:12.00 delay 1 second
@20:49:13.02 AFV.select :holdingCoil
@20:49:13.13 delay 1 second
@20:49:14.15 AS.reconfigure ASfast
@20:49:14.20 AS.seek 0ml
@20:49:41.52 delay 3 seconds
@20:49:44.54 AFV.select :carrierSolution
@20:49:44.65 delay 2 seconds
@20:49:46.67 AS.reconfigure ASconfig
@20:49:46.72 AS.seek 1ml
@20:50:44.47 delay 1 second
@20:50:45.49 AFV.select :holdingCoil
@20:50:45.60 delay 1 second
@20:50:46.62 AS.reconfigure ASfast
@20:50:46.67 AS.seek 0ml
@20:51:13.99 delay 3 seconds
@20:51:17.02 AFV.select :carrierSolution
@20:51:17.12 delay 2 seconds
@20:51:19.14 AS.reconfigure ASconfig
@20:51:19.19 AS.seek 1ml
@20:52:16.94 delay 1 second
@20:52:17.96 AFV.select :holdingCoil
@20:52:18.06 delay 1 second
@20:52:19.08 AS.reconfigure ASnorm
@20:52:19.13 AS.seek 0ml
@20:53:09.84 Setting up PCR for ACOPP5 -- writing /var/log/esp/ACOPP521mar1920h.pcr
@20:53:09.85 Building PCR Slug from ASV with MM3 mix, PP5 primer
@20:53:09.86 Priming with carrier
@20:53:09.87 ATV.dial ARV
@20:53:10.50 ARV.dial :carrier
@20:53:10.84 AS.seek 0.5ml
@20:53:36.41 AFV.select :carrierSolution
@20:53:36.52 delay 2 seconds
@20:53:38.54 AS.reconfigure ASconfig
@20:53:38.59 AS.seek 1ml
@20:54:07.60 delay 1 second
@20:54:08.62 AFV.select :holdingCoil
@20:54:08.73 delay 1 second
@20:54:09.75 ATV.dial :waste
@20:54:11.51 AS.reconfigure ASnorm
@20:54:11.55 AS.seek 0ml
@20:55:02.24 AFV.select :carrierSolution
@20:55:02.35 delay 2 seconds
@20:55:04.37 AS.reconfigure ASconfig
@20:55:04.42 AS.seek 0.5ml
@20:55:33.44 delay 1 second
@20:55:34.46 AFV.select :holdingCoil
@20:55:34.57 Building Slug
@20:55:34.58 ATV.dial :ARV
@20:55:36.31 delay 2 seconds
@20:55:38.34 ARV.dial :air1
@20:55:38.73 delay 2 seconds
@20:55:40.80 AS.reconfigure ASslow2
@20:55:40.85 AS.seek 0.506ml
@20:55:47.27 delay 2 seconds
@20:55:49.30 ARV.dial :MM3
@20:55:50.36 delay 2 seconds
@20:55:52.39 Adding MM3
@20:55:52.45 AS.seek 0.524ml
@20:56:10.83 delay 25 seconds
@20:56:35.86 ARV.dial :PP5
@20:56:36.67 Adding PP5
@20:56:36.68 delay 2 seconds
@20:56:38.74 AS.seek 0.53ml
@20:56:45.47 delay 10 seconds
@20:56:55.51 ARV.dial :air3
@20:56:55.90 adding ARV air
@20:56:55.91 delay 2 seconds
@20:56:57.97 AS.seek 0.536ml
@20:57:04.19 delay 5 seconds
@20:57:09.22 ARV.dial :carrier
@20:57:10.52 delay 7 seconds
@20:57:17.60 AS.seek 0.594ml
@20:58:16.05 delay 5 seconds
@20:58:21.08 ATV.dial :ASV
@20:58:21.50 delay 2 seconds
@20:58:23.57 AS.seek 0.6ml
@20:58:29.83 delay 4 seconds
@20:58:33.86 ATV.dial ARV
@20:58:34.42 delay 2 seconds
@20:58:36.45 ARV.dial :carrier
@20:58:36.46 delay 2 seconds
@20:58:38.52 AS.seek 0.65ml
@20:59:28.99 delay 4 seconds
@20:59:33.02 ATV.dial PCR
@20:59:33.66 ASV.dial :waste1
@20:59:33.68 ARV.dial :air1
@20:59:34.09 Positioning Slug by 0.324ml
@20:59:34.16 AS.seek 0.326ml
@21:05:00.17 delay 5 seconds
@21:05:05.20 ATV.dial ARV
@21:05:06.00 ASV.dial :PCRlock
@21:05:06.39 delay 5 seconds
@21:05:11.42 Running ACOPP5 ProfileIPc thermocycling
@21:05:11.43 <PCR> PCR.program ProfileIPc
@21:05:11.53 ProfileIPc should complete in < 1:26:35
@21:05:11.54 PCR.run 1:53:13.75
@22:13:06.72 PCR.off
@22:13:07.27 <MFBPCR> PCR for ACOPP5 completed -- closed /var/log/esp/ACOPP521mar1920h.pcr
@22:13:07.33 Cleaning PCR
@22:13:07.34 AFV.select :carrierSolution
@22:13:07.44 delay 2 seconds
@22:13:09.46 AS.reconfigure ASconfig
@22:13:09.51 AS.seek 0.5ml
@22:13:19.80 delay 1 second
@22:13:20.82 AFV.select :holdingCoil
@22:13:20.93 ASV.dial :waste1
@22:13:21.32 ATV.dial :air3
@22:13:22.66 AS.reconfigure ASslow1
@22:13:22.70 AS.seek 0.51ml
@22:13:24.98 delay 2 seconds
@22:13:27.00 ATV.dial :bleach
@22:13:27.63 AS.seek 0.81ml
@22:14:28.22 delay 3 seconds
@22:14:31.25 ATV.dial :air3
@22:14:31.82 delay 3 seconds
@22:14:34.89 AS.seek 0.835ml
@22:14:40.19 delay 3 seconds
@22:14:43.22 ATV.dial :bleach
@22:14:43.85 AS.seek 0.82ml
@22:14:47.16 delay 3 seconds
@22:14:50.19 ARV.dial :waste
@22:14:50.80 delay 3 seconds
@22:14:53.83 ATV.dial :ARV
@22:14:54.75 AS.reconfigure ASconfig
@22:14:54.79 AS.seek 0.47ml
@22:15:15.18 AFV.select :carrierSolution
@22:15:15.29 delay 2 seconds
@22:15:17.31 AS.seek 1ml
@22:15:48.07 delay 1 second
@22:15:49.09 AFV.select :holdingCoil
@22:15:49.20 delay 1 second
@22:15:50.22 AS.reconfigure ASfast
@22:15:50.27 AS.seek 0ml
@22:16:17.58 AFV.select :carrierSolution
@22:16:17.69 delay 2 seconds
@22:16:19.71 AS.reconfigure ASconfig
@22:16:19.76 AS.seek 1ml
@22:17:17.49 delay 1 second
@22:17:18.51 AFV.select :holdingCoil
@22:17:18.62 delay 1 second
@22:17:19.64 AS.reconfigure ASfast
@22:17:19.69 AS.seek 0ml
@22:17:47.00 AFV.select :carrierSolution
@22:17:47.11 delay 2 seconds
@22:17:49.13 AS.reconfigure ASconfig
@22:17:49.18 AS.seek 1ml
@22:18:46.93 delay 1 second
@22:18:47.95 AFV.select :holdingCoil
@22:18:48.06 delay 1 second
@22:18:49.08 AS.reconfigure ASfast
@22:18:49.13 AS.seek 0ml
@22:19:16.46 ARV.dial between carrier and air1
@22:19:16.96 ATV.dial :air3
@22:19:18.30 AS.reconfigure ASslow1
@22:19:18.34 AS.seek 0.01ml
@22:19:20.63 delay 2 seconds
@22:19:22.66 ATV.dial :bleach
@22:19:23.28 AS.seek 0.81ml
@22:22:04.51 delay 3 seconds
@22:22:07.54 ATV.dial :air3
@22:22:08.10 delay 3 seconds
@22:22:11.17 AS.seek 0.835ml
@22:22:16.47 delay 3 seconds
@22:22:19.50 ATV.dial :bleach
@22:22:20.13 AS.seek 0.82ml
@22:22:23.43 delay 3 seconds
@22:22:26.46 ATV.dial PCR
@22:22:26.87 AS.reconfigure ASconfig
@22:22:26.91 AS.seek 0ml
@22:23:14.30 PCR.program :bleachProfile
@22:23:14.36 PCR program should complete in < 2:45
@22:23:14.37 PCR.run 10:00
@22:26:10.55 PCR.off
@22:26:10.57 AFV.select :carrierSolution
@22:26:10.68 delay 2 seconds
@22:26:12.70 AS.seek 1ml
@22:27:10.46 delay 1 second
@22:27:11.48 AFV.select :holdingCoil
@22:27:11.59 delay 1 second
@22:27:12.61 AS.reconfigure ASfast
@22:27:12.66 AS.seek 0ml
@22:27:39.96 delay 3 seconds
@22:27:42.98 AFV.select :carrierSolution
@22:27:43.09 delay 2 seconds
@22:27:45.11 AS.reconfigure ASconfig
@22:27:45.16 AS.seek 1ml
@22:28:42.90 delay 1 second
@22:28:43.92 AFV.select :holdingCoil
@22:28:44.03 delay 1 second
@22:28:45.05 AS.reconfigure ASfast
@22:28:45.10 AS.seek 0ml
@22:29:12.44 delay 3 seconds
@22:29:15.46 AFV.select :carrierSolution
@22:29:15.57 delay 2 seconds
@22:29:17.59 AS.reconfigure ASconfig
@22:29:17.64 AS.seek 1ml
@22:30:15.38 delay 1 second
@22:30:16.40 AFV.select :holdingCoil
@22:30:16.51 delay 1 second
@22:30:17.53 AS.reconfigure ASfast
@22:30:17.58 AS.seek 0ml
@22:30:44.91 delay 3 seconds
@22:30:47.93 AFV.select :carrierSolution
@22:30:48.04 delay 2 seconds
@22:30:50.06 AS.reconfigure ASconfig
@22:30:50.11 AS.seek 1ml
@22:31:47.85 delay 1 second
@22:31:48.87 AFV.select :holdingCoil
@22:31:48.97 delay 1 second
@22:31:49.99 AS.reconfigure ASfast
@22:31:50.04 AS.seek 0ml
@22:32:17.35 delay 3 seconds
@22:32:20.37 AFV.select :carrierSolution
@22:32:20.48 delay 2 seconds
@22:32:22.50 AS.reconfigure ASconfig
@22:32:22.55 AS.seek 1ml
@22:33:20.29 delay 1 second
@22:33:21.31 AFV.select :holdingCoil
@22:33:21.41 delay 1 second
@22:33:22.43 AS.reconfigure ASnorm
@22:33:22.48 AS.seek 0ml
@22:34:13.15 Setting up PCR for AIPc -- writing /var/log/esp/AIPc21mar1922h.pcr
@22:34:13.16 Building PCR Slug from ASV with MM3 mix, PP6 primer
@22:34:13.17 Priming with carrier
@22:34:13.18 ATV.dial ARV
@22:34:13.82 ARV.dial :carrier
@22:34:14.16 AS.seek 0.5ml
@22:34:39.76 AFV.select :carrierSolution
@22:34:39.87 delay 2 seconds
@22:34:41.89 AS.reconfigure ASconfig
@22:34:41.94 AS.seek 1ml
@22:35:10.94 delay 1 second
@22:35:11.96 AFV.select :holdingCoil
@22:35:12.07 delay 1 second
@22:35:13.09 ATV.dial :waste
@22:35:14.86 AS.reconfigure ASnorm
@22:35:14.90 AS.seek 0ml
@22:36:05.58 AFV.select :carrierSolution
@22:36:05.69 delay 2 seconds
@22:36:07.71 AS.reconfigure ASconfig
@22:36:07.76 AS.seek 0.5ml
@22:36:36.80 delay 1 second
@22:36:37.82 AFV.select :holdingCoil
@22:36:37.93 Building Slug
@22:36:37.94 ATV.dial :ARV
@22:36:39.66 delay 2 seconds
@22:36:41.69 ARV.dial :air1
@22:36:42.07 delay 2 seconds
@22:36:44.14 AS.reconfigure ASslow2
@22:36:44.18 AS.seek 0.506ml
@22:36:50.47 delay 2 seconds
@22:36:52.50 ARV.dial :MM3
@22:36:53.57 delay 2 seconds
@22:36:55.60 Adding MM3
@22:36:55.66 AS.seek 0.524ml
@22:37:14.08 delay 25 seconds
@22:37:39.11 ARV.dial :PP6
@22:37:39.50 Adding PP6
@22:37:39.51 delay 2 seconds
@22:37:41.57 AS.seek 0.53ml
@22:37:48.05 delay 10 seconds
@22:37:58.09 ARV.dial :air1
@22:37:58.94 adding ARV air
@22:37:58.95 delay 2 seconds
@22:38:01.42 AS.seek 0.536ml
@22:38:07.71 delay 5 seconds
@22:38:12.74 ARV.dial :carrier
@22:38:13.13 delay 7 seconds
@22:38:20.21 AS.seek 0.594ml
@22:39:18.97 delay 5 seconds
@22:39:24.00 ATV.dial :ASV
@22:39:24.41 delay 2 seconds
@22:39:26.48 AS.seek 0.6ml
@22:39:32.71 delay 4 seconds
@22:39:36.74 ATV.dial ARV
@22:39:37.30 delay 2 seconds
@22:39:39.32 ARV.dial :carrier
@22:39:39.33 delay 2 seconds
@22:39:41.39 AS.seek 0.65ml
@22:40:32.19 delay 4 seconds
@22:40:36.22 ATV.dial PCR
@22:40:36.85 ASV.dial :waste1
@22:40:36.87 ARV.dial :air1
@22:40:37.27 Positioning Slug by 0.324ml
@22:40:37.34 AS.seek 0.326ml
@22:46:03.12 delay 5 seconds
@22:46:08.15 ATV.dial ARV
@22:46:08.96 ASV.dial :PCRlock
@22:46:09.35 delay 5 seconds
@22:46:14.38 Running AIPc ProfileIPc thermocycling
@22:46:14.39 <PCR> PCR.program ProfileIPc
@22:46:14.49 ProfileIPc should complete in < 1:26:35
@22:46:14.50 PCR.run 1:53:13.75
@23:54:09.01 PCR.off
@23:54:09.56 <MFBPCR> PCR for AIPc completed -- closed /var/log/esp/AIPc21mar1922h.pcr
@23:54:09.58 Full MFB Decontamination
Decon extraction column and decon extSrc
@23:54:09.60 SPE.hold 2:46:40,55C
@23:54:09.64 ASV.dial :SPEdelivery
@23:54:10.31 delay 20 seconds
@23:54:30.34 AFV.select :carrierSolution
@23:54:30.45 delay 2 seconds
@23:54:32.47 AS.reconfigure ASconfig
@23:54:32.52 AS.seek 0.5ml
@23:54:42.81 delay 1 second
@23:54:43.83 AFV.select :holdingCoil
@23:54:43.94 ATV.dial :air1
@23:54:46.19 AS.reconfigure ASslow1
@23:54:46.23 AS.seek 0.52ml
@23:54:50.59 ATV.dial :ASV
@23:54:52.34 ASV.dial :SPErecovery
@23:54:53.02 AS.seek 0.62ml
@23:55:13.56 ATV.dial :waste
@23:55:15.75 AS.reconfigure ASnorm
@23:55:15.79 AS.seek 0ml
@23:55:47.35 deconEluateRecoveryLine extSrc
@23:55:47.36 ATV.dial :waste
@23:55:47.38 AS.seek 0ml
@23:55:47.44 ATV.dial :air3
@23:55:48.19 AS.reconfigure ASslow1
@23:55:48.23 AS.seek 0.01ml
@23:55:50.52 delay 2 seconds
@23:55:52.55 ATV.dial :bleach
@23:55:53.04 AS.seek 0.31ml
@23:56:53.62 delay 3 seconds
@23:56:56.65 ATV.dial :air3
@23:56:57.26 delay 3 seconds
@23:57:00.33 AS.seek 0.335ml
@23:57:05.69 delay 3 seconds
@23:57:08.72 ATV.dial :bleach
@23:57:09.38 AS.seek 0.32ml
@23:57:12.62 delay 3 seconds
@23:57:15.65 ATV.dial :extSrc
@23:57:16.32 AS.seek 0ml
@23:58:20.93 AFV.select :carrierSolution
@23:58:21.04 delay 2 seconds
@23:58:23.06 AS.reconfigure ASconfig
@23:58:23.11 AS.seek 0.5ml
@23:58:52.13 delay 1 second
@23:58:53.15 AFV.select :holdingCoil
@23:58:53.26 ATV.dial :extSrc
@23:58:53.27 AS.reconfigure ASslow1
@23:58:53.32 AS.seek 0ml
@00:00:34.12CET20-Mar-21 AFV.select :carrierSolution
@00:00:34.23 delay 2 seconds
@00:00:36.25 AS.reconfigure ASconfig
@00:00:36.30 AS.seek 0.5ml
@00:01:05.32 delay 1 second
@00:01:06.34 AFV.select :holdingCoil
@00:01:06.45 ATV.dial :extSrc
@00:01:06.46 AS.reconfigure ASslow1
@00:01:06.51 AS.seek 0ml
@00:02:47.31 AFV.select :carrierSolution
@00:02:47.42 delay 2 seconds
@00:02:49.44 AS.reconfigure ASconfig
@00:02:49.49 AS.seek 0.5ml
@00:03:18.51 delay 1 second
@00:03:19.53 AFV.select :holdingCoil
@00:03:19.64 ATV.dial :extSrc
@00:03:19.65 AS.reconfigure ASslow1
@00:03:19.70 AS.seek 0ml
@00:05:00.51 AFV.select :carrierSolution
@00:05:00.62 delay 2 seconds
@00:05:02.64 AS.reconfigure ASconfig
@00:05:02.69 AS.seek 0.5ml
@00:05:31.71 delay 1 second
@00:05:32.73 AFV.select :holdingCoil
@00:05:32.84 ATV.dial :extSrc
@00:05:32.85 AS.reconfigure ASslow1
@00:05:32.90 AS.seek 0ml
@00:07:13.71 ATV.dial :air2
@00:07:15.12 AS.reconfigure ASslow0
@00:07:15.16 AS.seek 0.5ml
@00:08:05.71 ATV.dial :extSrc
@00:08:07.27 AS.seek 0.02ml
@00:08:55.85 delay 1 second
@00:08:56.88 ATV.dial :waste
@00:08:58.24 AS.reconfigure ASconfig
@00:08:58.28 AS.seek 0ml
@00:08:59.71 AFV.select :holdingCoil
@00:08:59.72 ATV.dial :waste
@00:08:59.74 AS.seek 0ml
@00:08:59.84 AFV.select :carrierSolution
@00:08:59.95 delay 2 seconds
@00:09:01.97 AS.seek 0.5ml
@00:09:31.01 delay 1 second
@00:09:32.03 AFV.select :holdingCoil
@00:09:32.14 ATV.dial :air3
@00:09:33.01 AS.reconfigure ASslow1
@00:09:33.05 AS.seek 0.52ml
@00:09:37.32 ATV.dial :air3
@00:09:37.39 AS.seek 0.53ml
@00:09:39.65 delay 2 seconds
@00:09:41.68 ATV.dial :bleach
@00:09:42.58 AS.seek 0.71ml
@00:10:19.03 delay 3 seconds
@00:10:22.06 ATV.dial :air3
@00:10:22.65 delay 3 seconds
@00:10:25.73 AS.seek 0.735ml
@00:10:31.02 delay 3 seconds
@00:10:34.05 ATV.dial :bleach
@00:10:34.68 AS.seek 0.72ml
@00:10:38.10 delay 3 seconds
@00:10:41.13 ATV.dial ASV
@00:10:42.23 ASV.dial :SPErecovery
@00:10:42.30 AS.seek 0.365ml
@00:11:53.96 ATV.dial :waste
@00:11:55.97 AS.reconfigure ASnorm
@00:11:56.01 AS.seek 0ml
@00:12:14.73 ATV.dial :air3
@00:12:15.60 AS.reconfigure ASslow1
@00:12:15.64 AS.seek 0.01ml
@00:12:17.93 delay 2 seconds
@00:12:19.96 ATV.dial :bleach
@00:12:20.49 AS.seek 0.58ml
@00:14:15.37 delay 3 seconds
@00:14:18.40 ATV.dial :air3
@00:14:18.98 delay 3 seconds
@00:14:22.05 AS.seek 0.605ml
@00:14:27.36 delay 3 seconds
@00:14:30.39 ATV.dial :bleach
@00:14:31.04 AS.seek 0.59ml
@00:14:34.35 delay 3 seconds
@00:14:37.38 ATV.dial :ASV
@00:14:38.48 ASV.dial :wasteR
@00:14:38.88 AS.seek 0ml
@00:16:37.87 delay 1:00
@00:17:37.91 ATV.dial ASV
@00:17:37.92 AFV.select :carrierSolution
@00:17:38.02 delay 2 seconds
@00:17:40.04 AS.reconfigure ASconfig
@00:17:40.09 AS.seek 1ml
@00:18:37.82 delay 1 second
@00:18:38.84 AFV.select :holdingCoil
@00:18:38.95 delay 1 second
@00:18:39.97 ASV.dial :wasteR
@00:18:39.98 AS.reconfigure ASslow1
@00:18:40.03 AS.seek 0ml
@00:22:01.40 delay 10 seconds
@00:22:11.44 ATV.dial :air3
@00:22:13.02 AS.seek 0.01ml
@00:22:15.31 delay 2 seconds
@00:22:17.34 ATV.dial :bleach
@00:22:17.96 AS.seek 0.58ml
@00:24:12.85 delay 3 seconds
@00:24:15.88 ATV.dial :air3
@00:24:16.45 delay 3 seconds
@00:24:19.52 AS.seek 0.605ml
@00:24:24.83 delay 3 seconds
@00:24:27.86 ATV.dial :bleach
@00:24:28.49 AS.seek 0.59ml
@00:24:31.82 delay 3 seconds
@00:24:34.85 ATV.dial ASV
@00:24:35.95 ASV.dial :SPEdelivery
@00:24:36.33 AS.seek 0ml
@00:26:35.23 delay 1:00
@00:27:35.26 AFV.select :carrierSolution
@00:27:35.37 delay 2 seconds
@00:27:37.39 AS.reconfigure ASconfig
@00:27:37.44 AS.seek 1ml
@00:28:35.18 delay 1 second
@00:28:36.20 AFV.select :holdingCoil
@00:28:36.31 delay 1 second
@00:28:37.33 ATV.dial ASV
@00:28:37.34 ASV.dial :SPEdelivery
@00:28:37.35 AS.reconfigure ASslow1
@00:28:37.40 AS.seek 0ml
@00:31:58.76 delay 10 seconds
@00:32:08.80 ATV.dial :air2
@00:32:09.69 AS.reconfigure ASslow0
@00:32:09.73 AS.seek 0.8ml
@00:33:30.45 delay 3 seconds
@00:33:33.48 ATV.dial :ASV
@00:33:34.51 ASV.dial :SPEdelivery
@00:33:34.53 AS.reconfigure ASslow1
@00:33:34.57 AS.seek 0.03ml
@00:36:09.70 delay 20 seconds
@00:36:29.74 ATV.dial :waste,via: :air3
@00:36:31.60 AS.reconfigure ASconfig
@00:36:31.64 AS.seek 0ml
@00:36:33.65 SPE.coast
@00:36:33.72 Removing Bleach from Mixing Coil
@00:36:33.73 AFV.select :carrierSolution
@00:36:33.83 delay 2 seconds
@00:36:35.85 AS.seek 0.5ml
@00:37:04.85 delay 1 second
@00:37:05.87 AFV.select :holdingCoil
@00:37:05.98 ATV.dial :ASV
@00:37:07.78 ASV.dial :SPErecovery
@00:37:08.43 AS.reconfigure ASslow1
@00:37:08.47 AS.seek 0.73ml
@00:37:55.00 ASV.dial :wasteD
@00:37:55.60 AS.reconfigure ASnorm
@00:37:55.64 AS.seek 0ml
@00:38:32.75 AFV.select :carrierSolution
@00:38:32.86 delay 2 seconds
@00:38:34.88 AS.reconfigure ASconfig
@00:38:34.93 AS.seek 0.5ml
@00:39:03.95 delay 1 second
@00:39:04.97 AFV.select :holdingCoil
@00:39:05.08 ATV.dial ASV
@00:39:05.09 ASV.dial :SPErecovery
@00:39:05.74 AS.reconfigure ASslow0
@00:39:05.78 AS.seek 0.3ml
@00:39:26.62 ATV.dial :air2
@00:39:27.73 AS.reconfigure ASslow1
@00:39:27.77 AS.seek 0.32ml
@00:39:32.12 ATV.dial ASV
@00:39:33.21 AS.reconfigure ASslow0
@00:39:33.25 AS.seek 0.57ml
@00:39:58.64 ASV.dial :wasteD
@00:39:59.24 AS.reconfigure ASnorm
@00:39:59.28 AS.seek 0ml
@00:40:28.32 AFV.select :carrierSolution
@00:40:28.43 delay 2 seconds
@00:40:30.45 AS.reconfigure ASconfig
@00:40:30.50 AS.seek 0.5ml
@00:40:59.51 delay 1 second
@00:41:00.53 AFV.select :holdingCoil
@00:41:00.64 ATV.dial ASV
@00:41:00.65 ASV.dial :SPErecovery
@00:41:01.29 AS.reconfigure ASslow0
@00:41:01.33 AS.seek 0.3ml
@00:41:21.74 ATV.dial :air2
@00:41:22.82 AS.reconfigure ASslow1
@00:41:22.86 AS.seek 0.32ml
@00:41:27.20 ATV.dial ASV
@00:41:28.29 AS.reconfigure ASslow0
@00:41:28.33 AS.seek 0.57ml
@00:41:53.79 ASV.dial :wasteD
@00:41:54.40 AS.reconfigure ASnorm
@00:41:54.44 AS.seek 0ml
@00:42:23.50 ATV.dial :air2
@00:42:24.54 AS.reconfigure ASslow0
@00:42:24.58 AS.seek 0.3ml
@00:42:55.07 ATV.dial :ASV
@00:42:56.10 ASV.dial :wasteD
@00:42:56.12 AS.reconfigure ASconfig
@00:42:56.16 AS.seek 0.05ml
@00:43:10.84 ATV.dial :waste
@00:43:12.83 AS.seek 0ml
@00:43:15.97 ASV.dial :SPEdelivery
@00:43:17.06 Cleaning PCR
AFV.select :carrierSolution
@00:43:17.17 delay 2 seconds
@00:43:19.19 AS.seek 0.5ml
@00:43:48.23 delay 1 second
@00:43:49.25 AFV.select :holdingCoil
@00:43:49.36 ASV.dial :waste1
@00:43:49.73 ATV.dial :air3
@00:43:50.45 AS.reconfigure ASslow1
@00:43:50.49 AS.seek 0.51ml
@00:43:52.87 delay 2 seconds
@00:43:54.90 ATV.dial :bleach
@00:43:55.35 AS.seek 0.81ml
@00:44:55.92 delay 3 seconds
@00:44:58.95 ATV.dial :air3
@00:44:59.51 delay 3 seconds
@00:45:02.58 AS.seek 0.835ml
@00:45:07.91 delay 3 seconds
@00:45:10.94 ATV.dial :bleach
@00:45:11.57 AS.seek 0.82ml
@00:45:14.84 delay 3 seconds
@00:45:17.87 ARV.dial :waste
@00:45:18.48 delay 3 seconds
@00:45:21.51 ATV.dial :ARV
@00:45:22.43 AS.reconfigure ASconfig
@00:45:22.47 AS.seek 0.47ml
@00:45:42.89 AFV.select :carrierSolution
@00:45:43.00 delay 2 seconds
@00:45:45.02 AS.seek 1ml
@00:46:15.75 delay 1 second
@00:46:16.77 AFV.select :holdingCoil
@00:46:16.88 delay 1 second
@00:46:17.90 AS.reconfigure ASfast
@00:46:17.95 AS.seek 0ml
@00:46:45.29 AFV.select :carrierSolution
@00:46:45.40 delay 2 seconds
@00:46:47.42 AS.reconfigure ASconfig
@00:46:47.47 AS.seek 1ml
@00:47:45.20 delay 1 second
@00:47:46.22 AFV.select :holdingCoil
@00:47:46.33 delay 1 second
@00:47:47.35 AS.reconfigure ASfast
@00:47:47.40 AS.seek 0ml
@00:48:14.73 AFV.select :carrierSolution
@00:48:14.84 delay 2 seconds
@00:48:16.86 AS.reconfigure ASconfig
@00:48:16.91 AS.seek 1ml
@00:49:14.68 delay 1 second
@00:49:15.70 AFV.select :holdingCoil
@00:49:15.81 delay 1 second
@00:49:16.83 AS.reconfigure ASfast
@00:49:16.88 AS.seek 0ml
@00:49:44.20 ARV.dial between carrier and air1
@00:49:44.71 ATV.dial :air3
@00:49:46.04 AS.reconfigure ASslow1
@00:49:46.08 AS.seek 0.01ml
@00:49:48.40 delay 2 seconds
@00:49:50.43 ATV.dial :bleach
@00:49:51.06 AS.seek 0.81ml
@00:52:32.17 delay 3 seconds
@00:52:35.20 ATV.dial :air3
@00:52:35.76 delay 3 seconds
@00:52:38.83 AS.seek 0.835ml
@00:52:44.10 delay 3 seconds
@00:52:47.13 ATV.dial :bleach
@00:52:47.76 AS.seek 0.82ml
@00:52:51.07 delay 3 seconds
@00:52:54.10 ATV.dial PCR
@00:52:54.52 AS.reconfigure ASconfig
@00:52:54.99 AS.seek 0ml
@00:53:42.39 PCR.program :bleachProfile
@00:53:42.45 PCR program should complete in < 2:45
@00:53:42.46 PCR.run 10:00
@00:56:44.30 PCR.off
@00:56:44.34 AFV.select :carrierSolution
@00:56:44.44 delay 2 seconds
@00:56:46.46 AS.seek 1ml
@00:57:44.23 delay 1 second
@00:57:45.25 AFV.select :holdingCoil
@00:57:45.36 delay 1 second
@00:57:46.38 AS.reconfigure ASfast
@00:57:46.43 AS.seek 0ml
@00:58:13.75 delay 3 seconds
@00:58:16.77 AFV.select :carrierSolution
@00:58:16.88 delay 2 seconds
@00:58:18.90 AS.reconfigure ASconfig
@00:58:18.95 AS.seek 1ml
@00:59:16.68 delay 1 second
@00:59:17.70 AFV.select :holdingCoil
@00:59:17.81 delay 1 second
@00:59:18.83 AS.reconfigure ASfast
@00:59:18.88 AS.seek 0ml
@00:59:46.21 delay 3 seconds
@00:59:49.23 AFV.select :carrierSolution
@00:59:49.34 delay 2 seconds
@00:59:51.36 AS.reconfigure ASconfig
@00:59:51.41 AS.seek 1ml
@01:00:49.17 delay 1 second
@01:00:50.19 AFV.select :holdingCoil
@01:00:50.30 delay 1 second
@01:00:51.32 AS.reconfigure ASfast
@01:00:51.37 AS.seek 0ml
@01:01:18.67 delay 3 seconds
@01:01:21.69 AFV.select :carrierSolution
@01:01:21.80 delay 2 seconds
@01:01:23.82 AS.reconfigure ASconfig
@01:01:23.87 AS.seek 1ml
@01:02:21.64 delay 1 second
@01:02:22.66 AFV.select :holdingCoil
@01:02:22.77 delay 1 second
@01:02:23.79 AS.reconfigure ASfast
@01:02:23.84 AS.seek 0ml
@01:02:51.18 delay 3 seconds
@01:02:54.20 AFV.select :carrierSolution
@01:02:54.31 delay 2 seconds
@01:02:56.33 AS.reconfigure ASconfig
@01:02:56.39 AS.seek 1ml
@01:03:54.11 delay 1 second
@01:03:55.13 AFV.select :holdingCoil
@01:03:55.24 delay 1 second
@01:03:56.26 AS.reconfigure ASnorm
@01:03:56.31 AS.seek 0ml
@01:04:47.03 AFV.select :carrierSolution
@01:04:47.13 delay 2 seconds
@01:04:49.15 AS.reconfigure ASconfig
@01:04:49.20 AS.seek 1ml
@01:05:46.98 delay 1 second
@01:05:48.00 AFV.select :holdingCoil
@01:05:48.11 delay 1 second
@01:05:49.13 ATV.dial :waste
@01:05:50.43 AS.reconfigure ASnorm
@01:05:50.47 AS.seek 0.5ml
@01:06:16.01 ATV.dial :waste2
@01:06:17.37 AS.seek 0ml
@15:26:09.78CET20-Mar-21 <MAIN> ESP2 v3.30 I2C Gateway v3.06 for core bus
@15:26:11.91 
Configuration for mer 7/20/20 brent@mbari.org
/home/esp/esp2/type/shallow/mer/configure.rb
@15:26:12.00 
Configuration for MFB3 by preston@mbari.org
/home/esp/esp2/type/mfb/MFB3/configure.rb
@15:26:16.42 Polling Can every 10:00
@15:26:18.12 CS.configure :default
@15:26:18.16 CS.hold
@15:26:18.22 CS.configure :default
@15:26:18.30 PS.configure :default
@15:26:18.38 SS.configure :default
@15:26:18.42 SS.hold
@15:26:18.48 SS.configure :default
@15:26:18.56 CC.configure :default
@15:26:18.60 CC.hold
@15:26:18.66 CC.configure :default
@15:26:18.74 PC.configure :default
@15:26:18.78 PC.hold
@15:26:18.85 PC.configure :default
@15:26:18.95 Forearm.configure :loose
@15:26:18.99 Forearm.hold
@15:26:19.04 Forearm.configure :loose
@15:26:19.12 Elbow.configure :loose
@15:26:19.16 Elbow.hold
@15:26:19.21 Elbow.configure :loose
@15:26:19.31 SC.configure :loose
@15:26:19.35 SC.hold
@15:26:19.40 SC.configure :loose
@15:26:19.48 SE.configure :default
@15:26:19.52 SE.hold
@15:26:19.56 SE.configure :default
@15:26:19.59 CH.coast
@15:26:19.64 CH.configure :default
@15:26:19.67 PH.coast
@15:26:19.71 PH.configure :default
@15:26:19.81 SPE.coast
@15:26:19.85 SPE.configure SPEconfig
@15:26:19.93 AS.configure ASconfig
@15:26:19.96 AS.hold
@15:26:20.01 AS.configure ASconfig
@15:26:20.05 PCR.off
@15:26:20.09 PCR.pidtemp= 50,0.1,0,300,25,25
@15:26:20.12 PCR.caltemp= 2.5051,0.0372,0
@15:26:20.15 All dwarf controllers running firmware version 3.75
@15:26:20.32 Cleanly exited at 15:25:47CET20-Mar-21
@15:26:20.35 Loading /home/esp/esp2/mission/GENOMAP.rb mission
@15:26:20.49 <MFBPCR> Extracting Nucleic Acid from extSrc
@15:26:20.52 SPE.hold 2:46:40,55C
@15:26:20.55 ASV.dial :SPEdelivery
@15:26:21.00 delay 20 seconds
@15:26:22.53 <mwj> Session Begun
@15:26:41.03 <MFBPCR> AFV.select :holdingCoil
@15:26:41.15 ATV.dial :air2
@15:26:42.42 AS.reconfigure ASslow1
@15:26:42.48 AS.seek 0.07ml
@15:26:56.87 delay 3 seconds
@15:26:59.90 ATV.dial :extSrc
@15:27:01.40 AS.seek 0.47ml
@15:28:22.15 delay 5 seconds
@15:28:27.22 Solid Phase Extraction Heater at 38.4C
@15:28:27.24 ATV.dial ASV
@15:28:28.12 ASV.dial :SPEdelivery
@15:28:28.21 AS.seek 0.042ml
@15:29:54.57 delay 10 seconds
@15:30:04.61 Rinsing external lysate line extSrc
@15:30:04.62 AFV.select :carrierSolution
@15:30:04.73 delay 2 seconds
@15:30:06.75 AS.reconfigure ASconfig
@15:30:06.81 AS.seek 1ml
@15:31:02.17 delay 1 second
@15:31:03.19 AFV.select :holdingCoil
@15:31:03.30 delay 1 second
@15:31:04.32 ATV.dial :extSrc
@15:31:05.15 AS.reconfigure ASnorm
@15:31:05.20 AS.seek 0ml
@15:31:55.89 ATV.dial :air2
@15:31:57.49 AS.reconfigure ASslow0
@15:31:57.55 AS.seek 0.05ml
@15:32:02.93 ATV.dial :extSrc
@15:32:04.51 AS.seek 0.01ml
@15:32:08.86 ATV.dial :waste
@15:32:10.24 AS.reconfigure ASconfig
@15:32:10.29 AS.seek 0ml
@15:32:11.15 ethanol wash
@15:32:11.49 ATV.dial :air2
@15:32:12.71 AS.reconfigure ASslow2
@15:32:12.76 AS.seek 0.01ml
@15:32:23.15 delay 3 seconds
@15:32:26.18 Pulling 0.4ml of Ethanol Wash
@15:32:26.19 ATV.dial :ethanol
@15:32:27.07 AS.reconfigure ASslow1
@15:32:27.13 AS.seek 0.41ml
@15:33:47.84 delay 3 seconds
@15:33:50.87 ATV.dial :air1
@15:33:51.29 delay 3 seconds
@15:33:54.36 AS.seek 0.435ml
@15:33:59.63 delay 3 seconds
@15:34:02.66 ATV.dial :ethanol
@15:34:03.28 AS.seek 0.42ml
@15:34:06.59 delay 3 seconds
@15:34:09.62 ATV.dial ASV
@15:34:11.03 AS.seek 0.093ml
@15:35:17.06 delay 3 seconds
@15:35:20.09 ATV.dial :waste2
@15:35:20.90 AS.reconfigure ASconfig
@15:35:20.95 AS.seek 0ml
@15:35:26.59 ATV.dial :air2
@15:35:27.04 AS.reconfigure ASslow1
@15:35:27.09 AS.seek 1ml
@15:38:48.56 delay 5 seconds
@15:38:53.59 ATV.dial ASV
@15:38:54.64 ASV.dial :SPEdelivery
@15:38:54.67 AS.seek 0.02ml
@15:42:12.04 delay 5 seconds
@15:42:17.07 ATV.dial :air2
@15:42:18.17 AS.seek 0.52ml
@15:43:59.03 delay 5 seconds
@15:44:04.06 ATV.dial ASV
@15:44:05.09 ASV.dial :wasteR
@15:44:05.49 AS.seek 0.3ml
@15:44:50.03 delay 20 seconds
@15:45:10.07 AS.seek 0.07ml
@15:45:56.64 delay 20 seconds
@15:46:16.67 ATV.dial :waste2
@15:46:17.48 AS.reconfigure ASconfig
@15:46:17.53 AS.seek 0ml
@15:46:21.86 Elution
@15:46:21.87 ATV.dial :air1
@15:46:23.07 AS.reconfigure ASslow1
@15:46:23.12 AS.seek 0.05ml
@15:46:33.47 delay 3 seconds
@15:46:36.50 ATV.dial :ElutionWater
@15:46:37.21 AS.seek 0.11ml
@15:46:49.53 delay 3 seconds
@15:46:52.56 ATV.dial ASV,via: :air2
@15:46:54.77 ASV.dial :SPEelution
@15:46:55.39 AFV.select :carrierSolution
@15:46:55.55 AS.reconfigure ASnorm
@15:46:55.61 AS.seek 0.76ml
@15:47:28.67 delay 3 seconds
@15:47:31.69 AFV.select :holdingCoil
@15:47:31.84 AS.reconfigure ASslow1
@15:47:31.90 AS.seek 0.475ml
@15:48:29.48 delay 5 seconds
@15:48:34.56 AS.reconfigure ASslow2
@15:48:34.62 AS.seek 0.44ml
@15:49:10.08 delay 1:00
@15:50:10.12 pushing eluate to holding coil
@15:50:10.19 AS.seek 0.28ml
@15:52:51.31 delay 30 seconds
@15:53:21.35 ATV.dial :waste,via: :air3
@15:53:23.20 AS.reconfigure ASconfig
@15:53:23.25 AS.seek 0ml
@15:53:39.64 Parking Bleach in LysateSource line and SPEcolumn
@15:53:39.66 ATV.dial :air3
@15:53:40.56 AS.reconfigure ASslow1
@15:53:40.61 AS.seek 0.03ml
@15:53:46.93 ATV.dial :ElutionWater
@15:53:47.57 AS.seek 0.06ml
@15:53:53.86 ATV.dial :air3
@15:53:54.51 AS.seek 0.07ml
@15:53:56.76 delay 2 seconds
@15:53:58.79 ATV.dial :bleach
@15:53:59.26 AS.seek 0.15ml
@15:54:15.61 delay 3 seconds
@15:54:18.64 ATV.dial :air3
@15:54:19.22 delay 3 seconds
@15:54:22.29 AS.seek 0.175ml
@15:54:27.62 delay 3 seconds
@15:54:30.65 ATV.dial :bleach
@15:54:31.28 AS.seek 0.16ml
@15:54:34.56 delay 3 seconds
@15:54:37.59 ATV.dial :extSrc
@15:54:38.26 AS.reconfigure ASconfig
@15:54:38.31 AS.seek 0.015ml
@15:54:46.95 ATV.dial :waste
@15:54:48.51 AS.seek 0ml
@15:54:49.64 ATV.dial :air3
@15:54:50.54 AS.reconfigure ASslow1
@15:54:50.59 AS.seek 0.01ml
@15:54:52.98 delay 2 seconds
@15:54:55.01 ATV.dial :bleach
@15:54:55.50 AS.seek 0.58ml
@15:56:50.40 delay 3 seconds
@15:56:53.43 ATV.dial :air3
@15:56:54.01 delay 3 seconds
@15:56:57.08 AS.seek 0.605ml
@15:57:02.40 delay 3 seconds
@15:57:05.43 ATV.dial :bleach
@15:57:06.07 AS.seek 0.59ml
@15:57:09.40 delay 3 seconds
@15:57:12.43 ATV.dial :ASV
@15:57:13.53 ASV.dial :wasteR
@15:57:14.15 AS.seek 0ml
@15:59:13.08 delay 1:00
@16:00:13.11 AFV.select :carrierSolution
@16:00:13.22 delay 2 seconds
@16:00:15.24 AS.reconfigure ASconfig
@16:00:15.29 AS.seek 1ml
@16:01:13.06 delay 1 second
@16:01:14.08 AFV.select :holdingCoil
@16:01:14.20 delay 1 second
@16:01:15.22 ATV.dial ASV
@16:01:15.23 ASV.dial :wasteR
@16:01:15.26 AS.reconfigure ASslow1
@16:01:15.32 AS.seek 0ml
@16:04:36.70 delay 20 seconds
@16:04:56.74 ATV.dial :air3
@16:04:58.34 AS.seek 0.59ml
@16:06:57.28 delay 3 seconds
@16:07:00.31 ATV.dial :ASV
@16:07:01.83 ASV.dial :wasteR
@16:07:01.92 AS.seek 0.09ml
@16:08:42.75 delay 30 seconds
@16:09:12.79 ATV.dial :extSrc
@16:09:13.59 delay 3 seconds
@16:09:16.62 ATV.dial :ElutionWater
@16:09:17.82 AS.seek 0.12ml
@16:09:24.14 ATV.dial :air3
@16:09:24.79 AS.seek 0.13ml
@16:09:27.06 delay 2 seconds
@16:09:29.09 ATV.dial :bleach
@16:09:29.58 AS.seek 0.23ml
@16:09:49.95 delay 3 seconds
@16:09:52.98 ATV.dial :air3
@16:09:53.56 delay 3 seconds
@16:09:56.63 AS.seek 0.255ml
@16:10:01.95 delay 3 seconds
@16:10:04.98 ATV.dial :bleach
@16:10:05.62 AS.seek 0.24ml
@16:10:08.86 delay 3 seconds
@16:10:12.32 ATV.dial :ASV
@16:10:13.42 ASV.dial :SPEdelivery
@16:10:13.83 AFV.select :carrierSolution
@16:10:13.96 delay 2 seconds
@16:10:15.98 AS.reconfigure ASconfig
@16:10:16.03 AS.seek 0.5ml
@16:10:31.31 delay 1 second
@16:10:32.33 AFV.select :holdingCoil
@16:10:32.48 AS.reconfigure ASslow1
@16:10:32.54 AS.seek 0.17ml
@16:11:39.21 SPE.hold 2:46:40,95C
@16:11:39.27 delay 3:00
@16:14:39.31 AFV.select :carrierSolution
@16:14:39.43 delay 2 seconds
@16:14:41.45 AS.reconfigure ASconfig
@16:14:41.50 AS.seek 1ml
@16:15:29.50 delay 1 second
@16:15:30.52 AFV.select :holdingCoil
@16:15:30.63 delay 1 second
@16:15:31.65 ASV.dial :wasteD
@16:15:32.76 AS.seek 0ml
@16:16:30.53 Priming Eluate
@16:16:30.54 AFV.select :carrierSolution
@16:16:30.66 delay 2 seconds
@16:16:32.68 AS.seek 0.5ml
@16:17:01.70 delay 1 second
@16:17:02.72 AFV.select :holdingCoil
@16:17:02.83 ATV.dial ASV
@16:17:02.84 ASV.dial :SPErecovery
@16:17:03.47 Swish #1 of 2
@16:17:03.55 AS.reconfigure ASslow0
@16:17:03.61 AS.seek 0.6ml
@16:17:14.07 AS.seek 0.5ml
@16:17:24.47 Swish #2 of 2
@16:17:24.56 AS.seek 0.6ml
@16:17:35.03 AS.seek 0.5ml
@16:17:45.44 delay 3 seconds
@16:17:48.51 AS.reconfigure ASslow1
@16:17:48.57 AS.seek 0.684ml
@16:18:25.89 delay 3 seconds
@16:18:28.92 Eluate primed
@16:18:28.93 ATV.dial :waste2
@16:18:29.62 AS.reconfigure ASnorm
@16:18:29.67 AS.seek 0ml
@16:19:04.48 SPE.coast
@16:19:04.56 ASV.dial :SPErecovery
@16:19:04.58 Setting up PCR for ACYPP2 -- writing /var/log/esp/ACYPP221mar2016h.pcr
@16:19:04.61 Building PCR Slug from ASV with MM1 mix, PP2 primer
@16:19:04.63 Priming with carrier
@16:19:04.65 ATV.dial ARV
@16:19:05.81 ARV.dial :carrier
@16:19:06.17 AS.seek 0.5ml
@16:19:31.75 AFV.select :carrierSolution
@16:19:31.88 delay 2 seconds
@16:19:33.90 AS.reconfigure ASconfig
@16:19:33.97 AS.seek 1ml
@16:20:03.00 delay 1 second
@16:20:04.02 AFV.select :holdingCoil
@16:20:04.13 delay 1 second
@16:20:05.15 ATV.dial :waste
@16:20:06.76 AS.reconfigure ASnorm
@16:20:06.82 AS.seek 0ml
@16:20:57.50 AFV.select :carrierSolution
@16:20:57.63 delay 2 seconds
@16:20:59.65 AS.reconfigure ASconfig
@16:20:59.70 AS.seek 0.5ml
@16:21:28.78 delay 1 second
@16:21:29.80 AFV.select :holdingCoil
@16:21:29.91 Building Slug
@16:21:29.92 ATV.dial :ARV
@16:21:31.70 delay 2 seconds
@16:21:33.72 ARV.dial :air1
@16:21:34.12 delay 2 seconds
@16:21:36.19 AS.reconfigure ASslow2
@16:21:36.25 AS.seek 0.506ml
@16:21:42.92 delay 2 seconds
@16:21:44.95 ARV.dial :MM1
@16:21:45.58 delay 2 seconds
@16:21:47.61 Adding MM1
@16:21:47.68 AS.seek 0.524ml
@16:22:05.87 delay 25 seconds
@16:22:30.90 ARV.dial :PP2
@16:22:31.32 Adding PP2
@16:22:31.33 delay 2 seconds
@16:22:33.40 AS.seek 0.53ml
@16:22:39.61 delay 10 seconds
@16:22:49.65 ARV.dial :air2
@16:22:50.07 adding ARV air
@16:22:50.08 delay 2 seconds
@16:22:52.16 AS.seek 0.536ml
@16:22:58.65 delay 5 seconds
@16:23:03.68 ARV.dial :carrier
@16:23:05.01 delay 7 seconds
@16:23:12.10 AS.seek 0.594ml
@16:24:10.94 delay 5 seconds
@16:24:15.97 ATV.dial :ASV
@16:24:16.39 delay 2 seconds
@16:24:18.47 AS.seek 0.6ml
@16:24:24.92 delay 4 seconds
@16:24:28.95 ATV.dial ARV
@16:24:29.51 delay 2 seconds
@16:24:31.54 ARV.dial :carrier
@16:24:31.55 delay 2 seconds
@16:24:33.63 AS.seek 0.65ml
@16:25:24.44 delay 4 seconds
@16:25:28.47 ATV.dial PCR
@16:25:29.11 ASV.dial :waste1
@16:25:29.93 ARV.dial :air1
@16:25:30.35 Positioning Slug by 0.324ml
@16:25:30.43 AS.seek 0.326ml
@16:30:56.36 delay 5 seconds
@16:31:01.39 ATV.dial ARV
@16:31:02.20 ASV.dial :PCRlock
@16:31:02.59 delay 5 seconds
@16:31:07.62 Running ACYPP2 Profile60CIQ2 thermocycling
@16:31:07.63 <PCR> PCR.program Profile60CIQ2
@16:31:07.76 Profile60CIQ2 should complete in < 1:47:50
@16:31:07.77 PCR.run 2:19:47.5
@17:59:07.05 PCR.off
@17:59:07.66 <MFBPCR> Running ACYPP2 MeltProfile60 thermocycling
@17:59:07.67 <PCR> PCR.program MeltProfile60
@17:59:07.78 MeltProfile60 should complete in < 11:55
@17:59:07.79 PCR.run 19:53.75
@18:06:30.45 PCR.off
@18:06:31.38 <MFBPCR> PCR for ACYPP2 completed -- closed /var/log/esp/ACYPP221mar2016h.pcr
@18:06:31.45 Cleaning PCR
@18:06:31.46 AFV.select :carrierSolution
@18:06:31.58 delay 2 seconds
@18:06:33.60 AS.reconfigure ASconfig
@18:06:33.65 AS.seek 0.5ml
@18:06:43.92 delay 1 second
@18:06:44.94 AFV.select :holdingCoil
@18:06:45.05 ASV.dial :waste1
@18:06:45.44 ATV.dial :air3
@18:06:46.81 AS.reconfigure ASslow1
@18:06:46.87 AS.seek 0.51ml
@18:06:49.14 delay 2 seconds
@18:06:51.17 ATV.dial :bleach
@18:06:51.83 AS.seek 0.81ml
@18:07:52.49 delay 3 seconds
@18:07:55.52 ATV.dial :air3
@18:07:56.10 delay 3 seconds
@18:07:59.17 AS.seek 0.835ml
@18:08:04.52 delay 3 seconds
@18:08:07.54 ATV.dial :bleach
@18:08:08.17 AS.seek 0.82ml
@18:08:11.47 delay 3 seconds
@18:08:14.50 ARV.dial :waste
@18:08:15.14 delay 3 seconds
@18:08:18.17 ATV.dial :ARV
@18:08:19.09 AS.reconfigure ASconfig
@18:08:19.15 AS.seek 0.47ml
@18:08:39.52 AFV.select :carrierSolution
@18:08:39.64 delay 2 seconds
@18:08:41.66 AS.seek 1ml
@18:09:12.46 delay 1 second
@18:09:13.48 AFV.select :holdingCoil
@18:09:13.59 delay 1 second
@18:09:14.61 AS.reconfigure ASfast
@18:09:14.66 AS.seek 0ml
@18:09:42.03 AFV.select :carrierSolution
@18:09:42.14 delay 2 seconds
@18:09:44.16 AS.reconfigure ASconfig
@18:09:44.21 AS.seek 1ml
@18:10:41.97 delay 1 second
@18:10:42.99 AFV.select :holdingCoil
@18:10:43.10 delay 1 second
@18:10:44.12 AS.reconfigure ASfast
@18:10:44.18 AS.seek 0ml
@18:11:11.52 AFV.select :carrierSolution
@18:11:11.64 delay 2 seconds
@18:11:13.66 AS.reconfigure ASconfig
@18:11:13.71 AS.seek 1ml
@18:12:11.49 delay 1 second
@18:12:12.51 AFV.select :holdingCoil
@18:12:12.62 delay 1 second
@18:12:13.64 AS.reconfigure ASfast
@18:12:13.69 AS.seek 0ml
@18:12:41.00 ARV.dial between carrier and air1
@18:12:41.53 ATV.dial :air3
@18:12:42.89 AS.reconfigure ASslow1
@18:12:42.95 AS.seek 0.01ml
@18:12:45.24 delay 2 seconds
@18:12:47.27 ATV.dial :bleach
@18:12:47.92 AS.seek 0.81ml
@18:15:29.06 delay 3 seconds
@18:15:32.09 ATV.dial :air3
@18:15:32.67 delay 3 seconds
@18:15:35.75 AS.seek 0.835ml
@18:15:41.04 delay 3 seconds
@18:15:44.07 ATV.dial :bleach
@18:15:44.70 AS.seek 0.82ml
@18:15:48.00 delay 3 seconds
@18:15:51.03 ATV.dial PCR
@18:15:51.45 AS.reconfigure ASconfig
@18:15:51.50 AS.seek 0ml
@18:16:38.93 PCR.program :bleachProfile
@18:16:39.06 PCR program should complete in < 2:45
@18:16:39.08 PCR.run 10:00
@18:19:38.37 PCR.off
@18:19:38.42 AFV.select :carrierSolution
@18:19:38.53 delay 2 seconds
@18:19:40.55 AS.seek 1ml
@18:20:38.32 delay 1 second
@18:20:39.34 AFV.select :holdingCoil
@18:20:39.45 delay 1 second
@18:20:40.47 AS.reconfigure ASfast
@18:20:40.53 AS.seek 0ml
@18:21:07.85 delay 3 seconds
@18:21:10.87 AFV.select :carrierSolution
@18:21:10.98 delay 2 seconds
@18:21:13.00 AS.reconfigure ASconfig
@18:21:13.06 AS.seek 1ml
@18:22:10.82 delay 1 second
@18:22:11.84 AFV.select :holdingCoil
@18:22:11.95 delay 1 second
@18:22:12.97 AS.reconfigure ASfast
@18:22:13.03 AS.seek 0ml
@18:22:40.38 delay 3 seconds
@18:22:43.40 AFV.select :carrierSolution
@18:22:43.51 delay 2 seconds
@18:22:45.53 AS.reconfigure ASconfig
@18:22:45.59 AS.seek 1ml
@18:23:43.33 delay 1 second
@18:23:44.35 AFV.select :holdingCoil
@18:23:44.46 delay 1 second
@18:23:45.48 AS.reconfigure ASfast
@18:23:45.54 AS.seek 0ml
@18:24:12.88 delay 3 seconds
@18:24:15.90 AFV.select :carrierSolution
@18:24:16.01 delay 2 seconds
@18:24:18.03 AS.reconfigure ASconfig
@18:24:18.08 AS.seek 1ml
@18:25:15.86 delay 1 second
@18:25:16.88 AFV.select :holdingCoil
@18:25:16.99 delay 1 second
@18:25:18.01 AS.reconfigure ASfast
@18:25:18.07 AS.seek 0ml
@18:25:45.42 delay 3 seconds
@18:25:48.44 AFV.select :carrierSolution
@18:25:48.55 delay 2 seconds
@18:25:50.57 AS.reconfigure ASconfig
@18:25:50.63 AS.seek 1ml
@18:26:48.38 delay 1 second
@18:26:49.40 AFV.select :holdingCoil
@18:26:49.51 delay 1 second
@18:26:50.53 AS.reconfigure ASnorm
@18:26:50.58 AS.seek 0ml
@18:27:41.30 Setting up PCR for AOPP1 -- writing /var/log/esp/AOPP121mar2018h.pcr
@18:27:41.31 Building PCR Slug from ASV with MM1 mix, PP1 primer
@18:27:41.34 Priming with carrier
@18:27:41.35 ATV.dial ARV
@18:27:42.00 ARV.dial :carrier
@18:27:42.34 AS.seek 0.5ml
@18:28:07.91 AFV.select :carrierSolution
@18:28:08.03 delay 2 seconds
@18:28:10.05 AS.reconfigure ASconfig
@18:28:10.11 AS.seek 1ml
@18:28:39.13 delay 1 second
@18:28:40.15 AFV.select :holdingCoil
@18:28:40.26 delay 1 second
@18:28:41.28 ATV.dial :waste
@18:28:43.06 AS.reconfigure ASnorm
@18:28:43.12 AS.seek 0ml
@18:29:33.80 AFV.select :carrierSolution
@18:29:33.92 delay 2 seconds
@18:29:35.94 AS.reconfigure ASconfig
@18:29:36.00 AS.seek 0.5ml
@18:30:05.02 delay 1 second
@18:30:06.04 AFV.select :holdingCoil
@18:30:06.15 Building Slug
@18:30:06.16 ATV.dial :ARV
@18:30:07.93 delay 2 seconds
@18:30:09.96 ARV.dial :air1
@18:30:10.36 delay 2 seconds
@18:30:12.43 AS.reconfigure ASslow2
@18:30:12.49 AS.seek 0.506ml
@18:30:18.97 delay 2 seconds
@18:30:21.00 ARV.dial :MM1
@18:30:21.63 delay 2 seconds
@18:30:23.66 Adding MM1
@18:30:23.73 AS.seek 0.524ml
@18:30:42.33 delay 25 seconds
@18:31:07.36 ARV.dial :PP1
@18:31:07.76 Adding PP1
@18:31:07.77 delay 2 seconds
@18:31:09.84 AS.seek 0.53ml
@18:31:16.30 delay 10 seconds
@18:31:26.34 ARV.dial :air1
@18:31:26.76 adding ARV air
@18:31:26.77 delay 2 seconds
@18:31:28.84 AS.seek 0.536ml
@18:31:35.22 delay 5 seconds
@18:31:40.25 ARV.dial :carrier
@18:31:40.66 delay 7 seconds
@18:31:47.73 AS.seek 0.594ml
@18:32:46.58 delay 5 seconds
@18:32:51.61 ATV.dial :ASV
@18:32:52.03 delay 2 seconds
@18:32:54.11 AS.seek 0.6ml
@18:33:00.38 delay 4 seconds
@18:33:04.41 ATV.dial ARV
@18:33:04.97 delay 2 seconds
@18:33:07.00 ARV.dial :carrier
@18:33:07.01 delay 2 seconds
@18:33:09.09 AS.seek 0.65ml
@18:33:59.60 delay 4 seconds
@18:34:03.63 ATV.dial PCR
@18:34:04.27 ASV.dial :waste1
@18:34:04.29 ARV.dial :air1
@18:34:04.73 Positioning Slug by 0.324ml
@18:34:04.81 AS.seek 0.326ml
@18:39:30.75 delay 5 seconds
@18:39:35.78 ATV.dial ARV
@18:39:36.58 ASV.dial :PCRlock
@18:39:36.99 delay 5 seconds
@18:39:42.02 Running AOPP1 Profile60CIQ1 thermocycling
@18:39:42.03 <PCR> PCR.program Profile60CIQ1
@18:39:42.15 Profile60CIQ1 should complete in < 1:56:15
@18:39:42.16 PCR.run 2:30:18.75
@20:18:07.95 PCR.off
@20:18:08.52 <MFBPCR> Running AOPP1 MeltProfile60 thermocycling
@20:18:08.53 <PCR> PCR.program MeltProfile60
@20:18:08.64 MeltProfile60 should complete in < 11:55
@20:18:08.65 PCR.run 19:53.75
@20:25:31.60 PCR.off
@20:25:32.52 <MFBPCR> PCR for AOPP1 completed -- closed /var/log/esp/AOPP121mar2018h.pcr
@20:25:32.59 Cleaning PCR
@20:25:32.60 AFV.select :carrierSolution
@20:25:32.72 delay 2 seconds
@20:25:34.74 AS.reconfigure ASconfig
@20:25:34.79 AS.seek 0.5ml
@20:25:45.07 delay 1 second
@20:25:46.09 AFV.select :holdingCoil
@20:25:46.20 ASV.dial :waste1
@20:25:46.59 ATV.dial :air3
@20:25:47.96 AS.reconfigure ASslow1
@20:25:48.02 AS.seek 0.51ml
@20:25:50.30 delay 2 seconds
@20:25:52.32 ATV.dial :bleach
@20:25:53.37 AS.seek 0.81ml
@20:26:53.95 delay 3 seconds
@20:26:56.98 ATV.dial :air3
@20:26:57.56 delay 3 seconds
@20:27:00.63 AS.seek 0.835ml
@20:27:05.94 delay 3 seconds
@20:27:08.97 ATV.dial :bleach
@20:27:09.62 AS.seek 0.82ml
@20:27:12.96 delay 3 seconds
@20:27:15.99 ARV.dial :waste
@20:27:16.63 delay 3 seconds
@20:27:19.66 ATV.dial :ARV
@20:27:20.60 AS.reconfigure ASconfig
@20:27:20.65 AS.seek 0.47ml
@20:27:41.06 AFV.select :carrierSolution
@20:27:41.18 delay 2 seconds
@20:27:43.20 AS.seek 1ml
@20:28:13.99 delay 1 second
@20:28:15.01 AFV.select :holdingCoil
@20:28:15.12 delay 1 second
@20:28:16.14 AS.reconfigure ASfast
@20:28:16.20 AS.seek 0ml
@20:28:43.54 AFV.select :carrierSolution
@20:28:43.66 delay 2 seconds
@20:28:45.68 AS.reconfigure ASconfig
@20:28:45.73 AS.seek 1ml
@20:29:43.51 delay 1 second
@20:29:44.53 AFV.select :holdingCoil
@20:29:44.64 delay 1 second
@20:29:45.66 AS.reconfigure ASfast
@20:29:45.72 AS.seek 0ml
@20:30:13.02 AFV.select :carrierSolution
@20:30:13.14 delay 2 seconds
@20:30:15.16 AS.reconfigure ASconfig
@20:30:15.21 AS.seek 1ml
@20:31:12.96 delay 1 second
@20:31:13.98 AFV.select :holdingCoil
@20:31:14.09 delay 1 second
@20:31:15.11 AS.reconfigure ASfast
@20:31:15.17 AS.seek 0ml
@20:31:42.51 ARV.dial between carrier and air1
@20:31:43.04 ATV.dial :air3
@20:31:44.41 AS.reconfigure ASslow1
@20:31:44.46 AS.seek 0.01ml
@20:31:46.84 delay 2 seconds
@20:31:48.87 ATV.dial :bleach
@20:31:49.52 AS.seek 0.81ml
@20:34:30.77 delay 3 seconds
@20:34:33.80 ATV.dial :air3
@20:34:34.38 delay 3 seconds
@20:34:37.45 AS.seek 0.835ml
@20:34:42.75 delay 3 seconds
@20:34:45.78 ATV.dial :bleach
@20:34:46.41 AS.seek 0.82ml
@20:34:49.65 delay 3 seconds
@20:34:52.68 ATV.dial PCR
@20:34:53.11 AS.reconfigure ASconfig
@20:34:53.16 AS.seek 0ml
@20:35:40.59 PCR.program :bleachProfile
@20:35:40.66 PCR program should complete in < 2:45
@20:35:40.67 PCR.run 10:00
@20:38:39.91 PCR.off
@20:38:39.94 AFV.select :carrierSolution
@20:38:40.06 delay 2 seconds
@20:38:42.08 AS.seek 1ml
@20:39:39.84 delay 1 second
@20:39:40.86 AFV.select :holdingCoil
@20:39:40.97 delay 1 second
@20:39:41.99 AS.reconfigure ASfast
@20:39:42.05 AS.seek 0ml
@20:40:09.38 delay 3 seconds
@20:40:12.40 AFV.select :carrierSolution
@20:40:12.51 delay 2 seconds
@20:40:14.53 AS.reconfigure ASconfig
@20:40:14.59 AS.seek 1ml
@20:41:12.35 delay 1 second
@20:41:13.37 AFV.select :holdingCoil
@20:41:13.48 delay 1 second
@20:41:14.50 AS.reconfigure ASfast
@20:41:14.56 AS.seek 0ml
@20:41:41.88 delay 3 seconds
@20:41:44.90 AFV.select :carrierSolution
@20:41:45.01 delay 2 seconds
@20:41:47.03 AS.reconfigure ASconfig
@20:41:47.08 AS.seek 1ml
@20:42:44.84 delay 1 second
@20:42:45.86 AFV.select :holdingCoil
@20:42:45.97 delay 1 second
@20:42:46.99 AS.reconfigure ASfast
@20:42:47.05 AS.seek 0ml
@20:43:14.40 delay 3 seconds
@20:43:17.42 AFV.select :carrierSolution
@20:43:17.53 delay 2 seconds
@20:43:19.55 AS.reconfigure ASconfig
@20:43:19.61 AS.seek 1ml
@20:44:17.35 delay 1 second
@20:44:18.37 AFV.select :holdingCoil
@20:44:18.48 delay 1 second
@20:44:19.50 AS.reconfigure ASfast
@20:44:19.56 AS.seek 0ml
@20:44:46.92 delay 3 seconds
@20:44:49.94 AFV.select :carrierSolution
@20:44:50.05 delay 2 seconds
@20:44:52.07 AS.reconfigure ASconfig
@20:44:52.12 AS.seek 1ml
@20:45:49.88 delay 1 second
@20:45:50.90 AFV.select :holdingCoil
@20:45:51.01 delay 1 second
@20:45:52.03 AS.reconfigure ASnorm
@20:45:52.08 AS.seek 0ml
@20:46:42.82 Setting up PCR for AARPP4 -- writing /var/log/esp/AARPP421mar2020h.pcr
@20:46:42.83 Building PCR Slug from ASV with MM2 mix, PP4 primer
@20:46:42.86 Priming with carrier
@20:46:42.88 ATV.dial ARV
@20:46:43.53 ARV.dial :carrier
@20:46:43.88 AS.seek 0.5ml
@20:47:09.43 AFV.select :carrierSolution
@20:47:09.55 delay 2 seconds
@20:47:11.57 AS.reconfigure ASconfig
@20:47:11.63 AS.seek 1ml
@20:47:40.65 delay 1 second
@20:47:41.67 AFV.select :holdingCoil
@20:47:41.78 delay 1 second
@20:47:42.80 ATV.dial :waste
@20:47:44.58 AS.reconfigure ASnorm
@20:47:44.64 AS.seek 0ml
@20:48:35.34 AFV.select :carrierSolution
@20:48:35.47 delay 2 seconds
@20:48:37.49 AS.reconfigure ASconfig
@20:48:37.55 AS.seek 0.5ml
@20:49:06.57 delay 1 second
@20:49:07.59 AFV.select :holdingCoil
@20:49:07.70 Building Slug
@20:49:07.71 ATV.dial :ARV
@20:49:09.48 delay 2 seconds
@20:49:11.51 ARV.dial :air1
@20:49:11.91 delay 2 seconds
@20:49:13.98 AS.reconfigure ASslow2
@20:49:14.04 AS.seek 0.506ml
@20:49:20.45 delay 2 seconds
@20:49:22.48 ARV.dial :MM2
@20:49:24.04 delay 2 seconds
@20:49:26.07 Adding MM2
@20:49:26.14 AS.seek 0.524ml
@20:49:44.98 delay 25 seconds
@20:50:10.01 ARV.dial :PP4
@20:50:10.43 Adding PP4
@20:50:10.44 delay 2 seconds
@20:50:12.51 AS.seek 0.53ml
@20:50:18.78 delay 10 seconds
@20:50:28.82 ARV.dial :air3
@20:50:29.22 adding ARV air
@20:50:29.23 delay 2 seconds
@20:50:31.30 AS.seek 0.536ml
@20:50:37.74 delay 5 seconds
@20:50:42.77 ARV.dial :carrier
@20:50:44.07 delay 7 seconds
@20:50:51.14 AS.seek 0.594ml
@20:51:49.84 delay 5 seconds
@20:51:54.87 ATV.dial :ASV
@20:51:55.29 delay 2 seconds
@20:51:57.36 AS.seek 0.6ml
@20:52:03.74 delay 4 seconds
@20:52:07.77 ATV.dial ARV
@20:52:08.45 delay 2 seconds
@20:52:10.48 ARV.dial :carrier
@20:52:10.49 delay 2 seconds
@20:52:12.56 AS.seek 0.65ml
@20:53:03.28 delay 4 seconds
@20:53:07.31 ATV.dial PCR
@20:53:07.95 ASV.dial :waste1
@20:53:07.97 ARV.dial :air1
@20:53:08.38 Positioning Slug by 0.324ml
@20:53:08.47 AS.seek 0.326ml
@20:58:34.57 delay 5 seconds
@20:58:39.60 ATV.dial ARV
@20:58:40.40 ASV.dial :PCRlock
@20:58:40.81 delay 5 seconds
@20:58:45.84 Running AARPP4 Profile58CIQ2 thermocycling
@20:58:45.85 <PCR> PCR.program Profile58CIQ2
@20:58:46.40 Profile58CIQ2 should complete in < 1:36:15
@20:58:46.41 PCR.run 2:05:18.75
@22:19:00.52 PCR.off
@22:19:01.09 <MFBPCR> Running AARPP4 MeltProfile58 thermocycling
@22:19:01.10 <PCR> PCR.program MeltProfile58
@22:19:01.20 MeltProfile58 should complete in < 12:33
@22:19:01.21 PCR.run 20:41.25
@22:26:33.50 PCR.off
@22:26:34.85 <MFBPCR> PCR for AARPP4 completed -- closed /var/log/esp/AARPP421mar2020h.pcr
@22:26:34.92 Cleaning PCR
@22:26:34.94 AFV.select :carrierSolution
@22:26:35.06 delay 2 seconds
@22:26:37.08 AS.reconfigure ASconfig
@22:26:37.13 AS.seek 0.5ml
@22:26:47.44 delay 1 second
@22:26:48.46 AFV.select :holdingCoil
@22:26:48.57 ASV.dial :waste1
@22:26:48.97 ATV.dial :air3
@22:26:50.34 AS.reconfigure ASslow1
@22:26:50.40 AS.seek 0.51ml
@22:26:52.68 delay 2 seconds
@22:26:54.71 ATV.dial :bleach
@22:26:55.37 AS.seek 0.81ml
@22:27:55.95 delay 3 seconds
@22:27:58.98 ATV.dial :air3
@22:27:59.56 delay 3 seconds
@22:28:02.63 AS.seek 0.835ml
@22:28:07.93 delay 3 seconds
@22:28:10.96 ATV.dial :bleach
@22:28:11.61 AS.seek 0.82ml
@22:28:15.06 delay 3 seconds
@22:28:18.09 ARV.dial :waste
@22:28:18.72 delay 3 seconds
@22:28:21.75 ATV.dial :ARV
@22:28:22.68 AS.reconfigure ASconfig
@22:28:22.73 AS.seek 0.47ml
@22:28:43.15 AFV.select :carrierSolution
@22:28:43.27 delay 2 seconds
@22:28:45.29 AS.seek 1ml
@22:29:16.08 delay 1 second
@22:29:17.10 AFV.select :holdingCoil
@22:29:17.21 delay 1 second
@22:29:18.23 AS.reconfigure ASfast
@22:29:18.29 AS.seek 0ml
@22:29:45.62 AFV.select :carrierSolution
@22:29:45.73 delay 2 seconds
@22:29:47.75 AS.reconfigure ASconfig
@22:29:47.80 AS.seek 1ml
@22:30:45.56 delay 1 second
@22:30:46.58 AFV.select :holdingCoil
@22:30:46.69 delay 1 second
@22:30:47.71 AS.reconfigure ASfast
@22:30:47.77 AS.seek 0ml
@22:31:15.08 AFV.select :carrierSolution
@22:31:15.20 delay 2 seconds
@22:31:17.22 AS.reconfigure ASconfig
@22:31:17.27 AS.seek 1ml
@22:32:15.03 delay 1 second
@22:32:16.05 AFV.select :holdingCoil
@22:32:16.16 delay 1 second
@22:32:17.18 AS.reconfigure ASfast
@22:32:17.24 AS.seek 0ml
@22:32:44.58 ARV.dial between carrier and air1
@22:32:45.09 ATV.dial :air3
@22:32:46.46 AS.reconfigure ASslow1
@22:32:46.52 AS.seek 0.01ml
@22:32:48.81 delay 2 seconds
@22:32:50.84 ATV.dial :bleach
@22:32:51.50 AS.seek 0.81ml
@22:35:32.64 delay 3 seconds
@22:35:35.67 ATV.dial :air3
@22:35:36.25 delay 3 seconds
@22:35:39.32 AS.seek 0.835ml
@22:35:44.63 delay 3 seconds
@22:35:47.66 ATV.dial :bleach
@22:35:48.29 AS.seek 0.82ml
@22:35:51.62 delay 3 seconds
@22:35:54.65 ATV.dial PCR
@22:35:55.09 AS.reconfigure ASconfig
@22:35:55.14 AS.seek 0ml
@22:36:42.58 PCR.program :bleachProfile
@22:36:42.65 PCR program should complete in < 2:45
@22:36:42.67 PCR.run 10:00
@22:39:41.92 PCR.off
@22:39:41.96 AFV.select :carrierSolution
@22:39:42.07 delay 2 seconds
@22:39:44.09 AS.seek 1ml
@22:40:41.86 delay 1 second
@22:40:42.88 AFV.select :holdingCoil
@22:40:42.99 delay 1 second
@22:40:44.01 AS.reconfigure ASfast
@22:40:44.07 AS.seek 0ml
@22:41:11.42 delay 3 seconds
@22:41:14.44 AFV.select :carrierSolution
@22:41:14.55 delay 2 seconds
@22:41:16.57 AS.reconfigure ASconfig
@22:41:16.63 AS.seek 1ml
@22:42:14.39 delay 1 second
@22:42:15.41 AFV.select :holdingCoil
@22:42:15.52 delay 1 second
@22:42:16.54 AS.reconfigure ASfast
@22:42:16.59 AS.seek 0ml
@22:42:43.94 delay 3 seconds
@22:42:46.96 AFV.select :carrierSolution
@22:42:47.07 delay 2 seconds
@22:42:49.09 AS.reconfigure ASconfig
@22:42:49.14 AS.seek 1ml
@22:43:46.89 delay 1 second
@22:43:47.91 AFV.select :holdingCoil
@22:43:48.02 delay 1 second
@22:43:49.04 AS.reconfigure ASfast
@22:43:49.10 AS.seek 0ml
@22:44:16.47 delay 3 seconds
@22:44:19.49 AFV.select :carrierSolution
@22:44:19.60 delay 2 seconds
@22:44:21.62 AS.reconfigure ASconfig
@22:44:21.68 AS.seek 1ml
@22:45:19.44 delay 1 second
@22:45:20.46 AFV.select :holdingCoil
@22:45:20.57 delay 1 second
@22:45:21.59 AS.reconfigure ASfast
@22:45:21.65 AS.seek 0ml
@22:45:49.00 delay 3 seconds
@22:45:52.02 AFV.select :carrierSolution
@22:45:52.13 delay 2 seconds
@22:45:54.15 AS.reconfigure ASconfig
@22:45:54.20 AS.seek 1ml
@22:46:51.95 delay 1 second
@22:46:52.97 AFV.select :holdingCoil
@22:46:53.08 delay 1 second
@22:46:54.10 AS.reconfigure ASnorm
@22:46:54.15 AS.seek 0ml
@22:47:44.87 Setting up PCR for AALPP3 -- writing /var/log/esp/AALPP321mar2022h.pcr
@22:47:44.88 Building PCR Slug from ASV with MM2 mix, PP3 primer
@22:47:44.91 Priming with carrier
@22:47:44.92 ATV.dial ARV
@22:47:45.58 ARV.dial :carrier
@22:47:45.90 AS.seek 0.5ml
@22:48:11.45 AFV.select :carrierSolution
@22:48:11.57 delay 2 seconds
@22:48:13.59 AS.reconfigure ASconfig
@22:48:13.65 AS.seek 1ml
@22:48:42.67 delay 1 second
@22:48:43.69 AFV.select :holdingCoil
@22:48:43.80 delay 1 second
@22:48:44.82 ATV.dial :waste
@22:48:46.60 AS.reconfigure ASnorm
@22:48:46.66 AS.seek 0ml
@22:49:37.37 AFV.select :carrierSolution
@22:49:37.49 delay 2 seconds
@22:49:39.51 AS.reconfigure ASconfig
@22:49:39.57 AS.seek 0.5ml
@22:50:08.64 delay 1 second
@22:50:09.66 AFV.select :holdingCoil
@22:50:09.77 Building Slug
@22:50:09.78 ATV.dial :ARV
@22:50:11.55 delay 2 seconds
@22:50:13.58 ARV.dial :air1
@22:50:13.98 delay 2 seconds
@22:50:16.05 AS.reconfigure ASslow2
@22:50:16.11 AS.seek 0.506ml
@22:50:22.52 delay 2 seconds
@22:50:24.55 ARV.dial :MM2
@22:50:26.11 delay 2 seconds
@22:50:28.14 Adding MM2
@22:50:28.21 AS.seek 0.524ml
@22:50:46.80 delay 25 seconds
@22:51:11.83 ARV.dial :PP3
@22:51:12.22 Adding PP3
@22:51:12.24 delay 2 seconds
@22:51:14.31 AS.seek 0.53ml
@22:51:20.81 delay 10 seconds
@22:51:30.85 ARV.dial :air2
@22:51:31.27 adding ARV air
@22:51:31.29 delay 2 seconds
@22:51:33.36 AS.seek 0.536ml
@22:51:40.00 delay 5 seconds
@22:51:45.03 ARV.dial :carrier
@22:51:46.34 delay 7 seconds
@22:51:53.42 AS.seek 0.594ml
@22:52:52.03 delay 5 seconds
@22:52:57.06 ATV.dial :ASV
@22:52:57.89 delay 2 seconds
@22:52:59.97 AS.seek 0.6ml
@22:53:06.45 delay 4 seconds
@22:53:10.48 ATV.dial ARV
@22:53:11.16 delay 2 seconds
@22:53:13.19 ARV.dial :carrier
@22:53:13.20 delay 2 seconds
@22:53:15.28 AS.seek 0.65ml
@22:54:05.92 delay 4 seconds
@22:54:09.95 ATV.dial PCR
@22:54:10.59 ASV.dial :waste1
@22:54:10.61 ARV.dial :air1
@22:54:11.03 Positioning Slug by 0.324ml
@22:54:11.12 AS.seek 0.326ml
@22:59:37.37 delay 5 seconds
@22:59:42.40 ATV.dial ARV
@22:59:43.20 ASV.dial :PCRlock
@22:59:43.61 delay 5 seconds
@22:59:48.64 Running AALPP3 Profile58CIQ2 thermocycling
@22:59:48.65 <PCR> PCR.program Profile58CIQ2
@22:59:48.77 Profile58CIQ2 should complete in < 1:36:15
@22:59:48.78 PCR.run 2:05:18.75
@00:19:56.52CET21-Mar-21 PCR.off
@00:19:57.47 <MFBPCR> Running AALPP3 MeltProfile58 thermocycling
@00:19:57.49 <PCR> PCR.program MeltProfile58
@00:19:57.59 MeltProfile58 should complete in < 12:33
@00:19:57.60 PCR.run 20:41.25
@00:27:30.51 PCR.off
@00:27:31.48 <MFBPCR> PCR for AALPP3 completed -- closed /var/log/esp/AALPP321mar2022h.pcr
@00:27:31.55 Cleaning PCR
@00:27:31.56 AFV.select :carrierSolution
@00:27:31.68 delay 2 seconds
@00:27:33.70 AS.reconfigure ASconfig
@00:27:33.75 AS.seek 0.5ml
@00:27:44.05 delay 1 second
@00:27:45.07 AFV.select :holdingCoil
@00:27:45.18 ASV.dial :waste1
@00:27:45.57 ATV.dial :air3
@00:27:46.92 AS.reconfigure ASslow1
@00:27:46.98 AS.seek 0.51ml
@00:27:49.28 delay 2 seconds
@00:27:51.30 ATV.dial :bleach
@00:27:51.94 AS.seek 0.81ml
@00:28:52.61 delay 3 seconds
@00:28:55.64 ATV.dial :air3
@00:28:56.22 delay 3 seconds
@00:28:59.29 AS.seek 0.835ml
@00:29:04.59 delay 3 seconds
@00:29:07.62 ATV.dial :bleach
@00:29:08.27 AS.seek 0.82ml
@00:29:11.60 delay 3 seconds
@00:29:14.63 ARV.dial :waste
@00:29:15.25 delay 3 seconds
@00:29:18.28 ATV.dial :ARV
@00:29:19.22 AS.reconfigure ASconfig
@00:29:19.27 AS.seek 0.47ml
@00:29:39.68 AFV.select :carrierSolution
@00:29:39.80 delay 2 seconds
@00:29:41.82 AS.seek 1ml
@00:30:12.58 delay 1 second
@00:30:13.60 AFV.select :holdingCoil
@00:30:13.71 delay 1 second
@00:30:14.73 AS.reconfigure ASfast
@00:30:14.79 AS.seek 0ml
@00:30:42.08 AFV.select :carrierSolution
@00:30:42.19 delay 2 seconds
@00:30:44.21 AS.reconfigure ASconfig
@00:30:44.26 AS.seek 1ml
@00:31:42.00 delay 1 second
@00:31:43.02 AFV.select :holdingCoil
@00:31:43.13 delay 1 second
@00:31:44.15 AS.reconfigure ASfast
@00:31:44.21 AS.seek 0ml
@00:32:11.57 AFV.select :carrierSolution
@00:32:11.69 delay 2 seconds
@00:32:13.71 AS.reconfigure ASconfig
@00:32:13.76 AS.seek 1ml
@00:33:11.50 delay 1 second
@00:33:12.52 AFV.select :holdingCoil
@00:33:12.63 delay 1 second
@00:33:13.65 AS.reconfigure ASfast
@00:33:13.70 AS.seek 0ml
@00:33:41.04 ARV.dial between carrier and air1
@00:33:41.57 ATV.dial :air3
@00:33:42.94 AS.reconfigure ASslow1
@00:33:43.00 AS.seek 0.01ml
@00:33:45.29 delay 2 seconds
@00:33:47.32 ATV.dial :bleach
@00:33:47.99 AS.seek 0.81ml
@00:36:29.14 delay 3 seconds
@00:36:32.17 ATV.dial :air3
@00:36:32.75 delay 3 seconds
@00:36:35.82 AS.seek 0.835ml
@00:36:41.11 delay 3 seconds
@00:36:44.14 ATV.dial :bleach
@00:36:44.78 AS.seek 0.82ml
@00:36:48.07 delay 3 seconds
@00:36:51.10 ATV.dial PCR
@00:36:51.53 AS.reconfigure ASconfig
@00:36:51.58 AS.seek 0ml
@00:37:38.99 PCR.program :bleachProfile
@00:37:39.06 PCR program should complete in < 2:45
@00:37:39.08 PCR.run 10:00
@00:40:38.32 PCR.off
@00:40:38.36 AFV.select :carrierSolution
@00:40:38.47 delay 2 seconds
@00:40:40.49 AS.seek 1ml
@00:41:38.29 delay 1 second
@00:41:39.31 AFV.select :holdingCoil
@00:41:39.42 delay 1 second
@00:41:40.44 AS.reconfigure ASfast
@00:41:40.50 AS.seek 0ml
@00:42:07.83 delay 3 seconds
@00:42:10.85 AFV.select :carrierSolution
@00:42:10.96 delay 2 seconds
@00:42:12.98 AS.reconfigure ASconfig
@00:42:13.05 AS.seek 1ml
@00:43:10.83 delay 1 second
@00:43:11.85 AFV.select :holdingCoil
@00:43:11.96 delay 1 second
@00:43:12.98 AS.reconfigure ASfast
@00:43:13.03 AS.seek 0ml
@00:43:40.38 delay 3 seconds
@00:43:43.40 AFV.select :carrierSolution
@00:43:43.51 delay 2 seconds
@00:43:45.53 AS.reconfigure ASconfig
@00:43:45.59 AS.seek 1ml
@00:44:43.35 delay 1 second
@00:44:44.37 AFV.select :holdingCoil
@00:44:44.48 delay 1 second
@00:44:45.50 AS.reconfigure ASfast
@00:44:45.56 AS.seek 0ml
@00:45:12.90 delay 3 seconds
@00:45:15.92 AFV.select :carrierSolution
@00:45:16.03 delay 2 seconds
@00:45:18.05 AS.reconfigure ASconfig
@00:45:18.11 AS.seek 1ml
@00:46:15.88 delay 1 second
@00:46:16.90 AFV.select :holdingCoil
@00:46:17.01 delay 1 second
@00:46:18.03 AS.reconfigure ASfast
@00:46:18.10 AS.seek 0ml
@00:46:45.43 delay 3 seconds
@00:46:48.45 AFV.select :carrierSolution
@00:46:48.56 delay 2 seconds
@00:46:50.58 AS.reconfigure ASconfig
@00:46:50.64 AS.seek 1ml
@00:47:48.43 delay 1 second
@00:47:49.45 AFV.select :holdingCoil
@00:47:49.56 delay 1 second
@00:47:50.58 AS.reconfigure ASnorm
@00:47:50.63 AS.seek 0ml
@00:48:41.36 Setting up PCR for ACOPP5 -- writing /var/log/esp/ACOPP521mar2100h.pcr
@00:48:41.37 Building PCR Slug from ASV with MM3 mix, PP5 primer
@00:48:41.40 Priming with carrier
@00:48:41.42 ATV.dial ARV
@00:48:42.07 ARV.dial :carrier
@00:48:42.42 AS.seek 0.5ml
@00:49:07.97 AFV.select :carrierSolution
@00:49:08.09 delay 2 seconds
@00:49:10.11 AS.reconfigure ASconfig
@00:49:10.17 AS.seek 1ml
@00:49:39.18 delay 1 second
@00:49:40.20 AFV.select :holdingCoil
@00:49:40.31 delay 1 second
@00:49:41.33 ATV.dial :waste
@00:49:43.11 AS.reconfigure ASnorm
@00:49:43.17 AS.seek 0ml
@00:50:33.86 AFV.select :carrierSolution
@00:50:33.98 delay 2 seconds
@00:50:36.00 AS.reconfigure ASconfig
@00:50:36.06 AS.seek 0.5ml
@00:51:05.09 delay 1 second
@00:51:06.10 AFV.select :holdingCoil
@00:51:06.21 Building Slug
@00:51:06.22 ATV.dial :ARV
@00:51:07.99 delay 2 seconds
@00:51:10.02 ARV.dial :air1
@00:51:10.42 delay 2 seconds
@00:51:12.49 AS.reconfigure ASslow2
@00:51:12.55 AS.seek 0.506ml
@00:51:19.04 delay 2 seconds
@00:51:21.48 ARV.dial :MM3
@00:51:22.57 delay 2 seconds
@00:51:24.60 Adding MM3
@00:51:24.68 AS.seek 0.524ml
@00:51:42.99 delay 25 seconds
@00:52:08.02 ARV.dial :PP5
@00:52:08.43 Adding PP5
@00:52:08.45 delay 2 seconds
@00:52:10.52 AS.seek 0.53ml
@00:52:16.72 delay 10 seconds
@00:52:26.76 ARV.dial :air3
@00:52:27.16 adding ARV air
@00:52:27.18 delay 2 seconds
@00:52:29.25 AS.seek 0.536ml
@00:52:35.82 delay 5 seconds
@00:52:40.85 ARV.dial :carrier
@00:52:42.16 delay 7 seconds
@00:52:49.24 AS.seek 0.594ml
@00:53:47.76 delay 5 seconds
@00:53:52.79 ATV.dial :ASV
@00:53:53.21 delay 2 seconds
@00:53:55.28 AS.seek 0.6ml
@00:54:01.54 delay 4 seconds
@00:54:05.57 ATV.dial ARV
@00:54:06.15 delay 2 seconds
@00:54:08.18 ARV.dial :carrier
@00:54:08.19 delay 2 seconds
@00:54:10.26 AS.seek 0.65ml
@00:55:01.08 delay 4 seconds
@00:55:05.11 ATV.dial PCR
@00:55:05.76 ASV.dial :waste1
@00:55:05.78 ARV.dial :air1
@00:55:06.20 Positioning Slug by 0.324ml
@00:55:06.29 AS.seek 0.326ml
@01:00:32.29 delay 5 seconds
@01:00:37.32 ATV.dial ARV
@01:00:38.13 ASV.dial :PCRlock
@01:00:38.54 delay 5 seconds
@01:00:43.57 Running ACOPP5 ProfileIPc thermocycling
@01:00:43.58 <PCR> PCR.program ProfileIPc
@01:00:43.70 ProfileIPc should complete in < 1:26:35
@01:00:43.71 PCR.run 1:53:13.75
@02:08:44.05 PCR.off
@02:08:44.60 <MFBPCR> PCR for ACOPP5 completed -- closed /var/log/esp/ACOPP521mar2100h.pcr
@02:08:44.67 Cleaning PCR
@02:08:44.69 AFV.select :carrierSolution
@02:08:44.81 delay 2 seconds
@02:08:46.83 AS.reconfigure ASconfig
@02:08:46.88 AS.seek 0.5ml
@02:08:57.15 delay 1 second
@02:08:58.17 AFV.select :holdingCoil
@02:08:58.28 ASV.dial :waste1
@02:08:58.68 ATV.dial :air3
@02:09:00.05 AS.reconfigure ASslow1
@02:09:00.11 AS.seek 0.51ml
@02:09:02.36 delay 2 seconds
@02:09:04.39 ATV.dial :bleach
@02:09:05.04 AS.seek 0.81ml
@02:10:05.65 delay 3 seconds
@02:10:08.68 ATV.dial :air3
@02:10:09.26 delay 3 seconds
@02:10:12.33 AS.seek 0.835ml
@02:10:17.64 delay 3 seconds
@02:10:20.67 ATV.dial :bleach
@02:10:21.30 AS.seek 0.82ml
@02:10:24.62 delay 3 seconds
@02:10:27.65 ARV.dial :waste
@02:10:28.27 delay 3 seconds
@02:10:31.30 ATV.dial :ARV
@02:10:32.24 AS.reconfigure ASconfig
@02:10:32.29 AS.seek 0.47ml
@02:10:52.71 AFV.select :carrierSolution
@02:10:52.83 delay 2 seconds
@02:10:54.85 AS.seek 1ml
@02:11:25.59 delay 1 second
@02:11:26.61 AFV.select :holdingCoil
@02:11:26.72 delay 1 second
@02:11:27.74 AS.reconfigure ASfast
@02:11:27.80 AS.seek 0ml
@02:11:55.13 AFV.select :carrierSolution
@02:11:55.25 delay 2 seconds
@02:11:57.27 AS.reconfigure ASconfig
@02:11:57.32 AS.seek 1ml
@02:12:55.07 delay 1 second
@02:12:56.09 AFV.select :holdingCoil
@02:12:56.20 delay 1 second
@02:12:57.22 AS.reconfigure ASfast
@02:12:57.27 AS.seek 0ml
@02:13:24.62 AFV.select :carrierSolution
@02:13:24.74 delay 2 seconds
@02:13:26.76 AS.reconfigure ASconfig
@02:13:26.81 AS.seek 1ml
@02:14:24.57 delay 1 second
@02:14:25.59 AFV.select :holdingCoil
@02:14:25.70 delay 1 second
@02:14:26.72 AS.reconfigure ASfast
@02:14:26.77 AS.seek 0ml
@02:14:54.10 ARV.dial between carrier and air1
@02:14:54.61 ATV.dial :air3
@02:14:55.96 AS.reconfigure ASslow1
@02:14:56.01 AS.seek 0.01ml
@02:14:58.41 delay 2 seconds
@02:15:00.44 ATV.dial :bleach
@02:15:01.08 AS.seek 0.81ml
@02:17:42.24 delay 3 seconds
@02:17:45.27 ATV.dial :air3
@02:17:45.85 delay 3 seconds
@02:17:48.92 AS.seek 0.835ml
@02:17:54.32 delay 3 seconds
@02:17:57.35 ATV.dial :bleach
@02:17:58.01 AS.seek 0.82ml
@02:18:01.31 delay 3 seconds
@02:18:04.34 ATV.dial PCR
@02:18:04.77 AS.reconfigure ASconfig
@02:18:04.82 AS.seek 0ml
@02:18:52.23 PCR.program :bleachProfile
@02:18:52.30 PCR program should complete in < 2:45
@02:18:52.31 PCR.run 10:00
@02:21:48.53 PCR.off
@02:21:48.58 AFV.select :carrierSolution
@02:21:48.69 delay 2 seconds
@02:21:50.71 AS.seek 1ml
@02:22:48.48 delay 1 second
@02:22:49.50 AFV.select :holdingCoil
@02:22:49.61 delay 1 second
@02:22:50.63 AS.reconfigure ASfast
@02:22:50.68 AS.seek 0ml
@02:23:18.04 delay 3 seconds
@02:23:21.06 AFV.select :carrierSolution
@02:23:21.17 delay 2 seconds
@02:23:23.19 AS.reconfigure ASconfig
@02:23:23.25 AS.seek 1ml
@02:24:20.99 delay 1 second
@02:24:22.01 AFV.select :holdingCoil
@02:24:22.12 delay 1 second
@02:24:23.14 AS.reconfigure ASfast
@02:24:23.20 AS.seek 0ml
@02:24:50.54 delay 3 seconds
@02:24:53.56 AFV.select :carrierSolution
@02:24:53.67 delay 2 seconds
@02:24:55.69 AS.reconfigure ASconfig
@02:24:55.75 AS.seek 1ml
@02:25:53.49 delay 1 second
@02:25:54.51 AFV.select :holdingCoil
@02:25:54.62 delay 1 second
@02:25:55.64 AS.reconfigure ASfast
@02:25:55.70 AS.seek 0ml
@02:26:23.02 delay 3 seconds
@02:26:26.04 AFV.select :carrierSolution
@02:26:26.15 delay 2 seconds
@02:26:28.17 AS.reconfigure ASconfig
@02:26:28.23 AS.seek 1ml
@02:27:25.98 delay 1 second
@02:27:27.00 AFV.select :holdingCoil
@02:27:27.11 delay 1 second
@02:27:28.13 AS.reconfigure ASfast
@02:27:28.18 AS.seek 0ml
@02:27:55.54 delay 3 seconds
@02:27:58.56 AFV.select :carrierSolution
@02:27:58.67 delay 2 seconds
@02:28:00.69 AS.reconfigure ASconfig
@02:28:00.75 AS.seek 1ml
@02:28:58.54 delay 1 second
@02:28:59.56 AFV.select :holdingCoil
@02:28:59.67 delay 1 second
@02:29:00.69 AS.reconfigure ASnorm
@02:29:00.74 AS.seek 0ml
@02:29:51.46 Setting up PCR for AIPc -- writing /var/log/esp/AIPc21mar2102h.pcr
@02:29:51.47 Building PCR Slug from ASV with MM3 mix, PP6 primer
@02:29:51.50 Priming with carrier
@02:29:51.51 ATV.dial ARV
@02:29:52.17 ARV.dial :carrier
@02:29:52.49 AS.seek 0.5ml
@02:30:18.06 AFV.select :carrierSolution
@02:30:18.18 delay 2 seconds
@02:30:20.20 AS.reconfigure ASconfig
@02:30:20.26 AS.seek 1ml
@02:30:49.29 delay 1 second
@02:30:50.31 AFV.select :holdingCoil
@02:30:50.42 delay 1 second
@02:30:51.44 ATV.dial :waste
@02:30:53.22 AS.reconfigure ASnorm
@02:30:53.28 AS.seek 0ml
@02:31:43.95 AFV.select :carrierSolution
@02:31:44.07 delay 2 seconds
@02:31:46.09 AS.reconfigure ASconfig
@02:31:46.15 AS.seek 0.5ml
@02:32:15.15 delay 1 second
@02:32:16.17 AFV.select :holdingCoil
@02:32:16.28 Building Slug
@02:32:16.29 ATV.dial :ARV
@02:32:18.04 delay 2 seconds
@02:32:20.07 ARV.dial :air1
@02:32:20.47 delay 2 seconds
@02:32:22.54 AS.reconfigure ASslow2
@02:32:22.60 AS.seek 0.506ml
@02:32:29.13 delay 2 seconds
@02:32:31.16 ARV.dial :MM3
@02:32:32.24 delay 2 seconds
@02:32:34.68 Adding MM3
@02:32:34.76 AS.seek 0.524ml
@02:32:53.20 delay 25 seconds
@02:33:18.23 ARV.dial :PP6
@02:33:18.63 Adding PP6
@02:33:18.65 delay 2 seconds
@02:33:20.72 AS.seek 0.53ml
@02:33:27.24 delay 10 seconds
@02:33:37.28 ARV.dial :air1
@02:33:38.13 adding ARV air
@02:33:38.15 delay 2 seconds
@02:33:40.22 AS.seek 0.536ml
@02:33:46.67 delay 5 seconds
@02:33:51.70 ARV.dial :carrier
@02:33:52.10 delay 7 seconds
@02:33:59.18 AS.seek 0.594ml
@02:34:57.88 delay 5 seconds
@02:35:02.91 ATV.dial :ASV
@02:35:03.32 delay 2 seconds
@02:35:05.39 AS.seek 0.6ml
@02:35:11.74 delay 4 seconds
@02:35:15.77 ATV.dial ARV
@02:35:16.35 delay 2 seconds
@02:35:18.38 ARV.dial :carrier
@02:35:18.39 delay 2 seconds
@02:35:20.46 AS.seek 0.65ml
@02:36:11.17 delay 4 seconds
@02:36:15.20 ATV.dial PCR
@02:36:15.84 ASV.dial :waste1
@02:36:15.86 ARV.dial :air1
@02:36:16.27 Positioning Slug by 0.324ml
@02:36:16.36 AS.seek 0.326ml
@02:41:42.51 delay 5 seconds
@02:41:47.54 ATV.dial ARV
@02:41:48.35 ASV.dial :PCRlock
@02:41:48.74 delay 5 seconds
@02:41:53.77 Running AIPc ProfileIPc thermocycling
@02:41:53.78 <PCR> PCR.program ProfileIPc
@02:41:53.91 ProfileIPc should complete in < 1:26:35
@02:41:53.92 PCR.run 1:53:13.75
@03:49:59.55 PCR.off
@03:50:00.11 <MFBPCR> PCR for AIPc completed -- closed /var/log/esp/AIPc21mar2102h.pcr
@03:50:00.13 Full MFB Decontamination
@03:50:00.15 Decon extraction column and decon extSrc
@03:50:00.18 SPE.hold 2:46:40,55C
@03:50:00.23 ASV.dial :SPEdelivery
@03:50:00.90 delay 20 seconds
@03:50:20.93 AFV.select :carrierSolution
@03:50:21.04 delay 2 seconds
@03:50:23.06 AS.reconfigure ASconfig
@03:50:23.11 AS.seek 0.5ml
@03:50:33.43 delay 1 second
@03:50:34.45 AFV.select :holdingCoil
@03:50:34.56 ATV.dial :air1
@03:50:36.81 AS.reconfigure ASslow1
@03:50:36.87 AS.seek 0.52ml
@03:50:41.23 ATV.dial :ASV
@03:50:43.00 ASV.dial :SPErecovery
@03:50:43.70 AS.seek 0.62ml
@03:51:04.15 ATV.dial :waste
@03:51:06.35 AS.reconfigure ASnorm
@03:51:06.40 AS.seek 0ml
@03:51:38.03 deconEluateRecoveryLine extSrc
@03:51:38.04 ATV.dial :waste
@03:51:38.08 AS.seek 0ml
@03:51:38.16 ATV.dial :air3
@03:51:38.95 AS.reconfigure ASslow1
@03:51:39.01 AS.seek 0.01ml
@03:51:41.34 delay 2 seconds
@03:51:43.37 ATV.dial :bleach
@03:51:43.87 AS.seek 0.31ml
@03:52:44.53 delay 3 seconds
@03:52:47.56 ATV.dial :air3
@03:52:48.17 delay 3 seconds
@03:52:51.24 AS.seek 0.335ml
@03:52:56.60 delay 3 seconds
@03:52:59.63 ATV.dial :bleach
@03:53:00.29 AS.seek 0.32ml
@03:53:03.50 delay 3 seconds
@03:53:06.53 ATV.dial :extSrc
@03:53:07.21 AS.seek 0ml
@03:54:11.84 AFV.select :carrierSolution
@03:54:11.96 delay 2 seconds
@03:54:13.98 AS.reconfigure ASconfig
@03:54:14.04 AS.seek 0.5ml
@03:54:43.08 delay 1 second
@03:54:44.10 AFV.select :holdingCoil
@03:54:44.21 ATV.dial :extSrc
@03:54:44.23 AS.reconfigure ASslow1
@03:54:44.30 AS.seek 0ml
@03:56:25.11 AFV.select :carrierSolution
@03:56:25.23 delay 2 seconds
@03:56:27.25 AS.reconfigure ASconfig
@03:56:27.30 AS.seek 0.5ml
@03:56:56.34 delay 1 second
@03:56:57.36 AFV.select :holdingCoil
@03:56:57.47 ATV.dial :extSrc
@03:56:57.49 AS.reconfigure ASslow1
@03:56:57.55 AS.seek 0ml
@03:58:38.36 AFV.select :carrierSolution
@03:58:38.48 delay 2 seconds
@03:58:40.50 AS.reconfigure ASconfig
@03:58:40.56 AS.seek 0.5ml
@03:59:09.58 delay 1 second
@03:59:10.60 AFV.select :holdingCoil
@03:59:10.71 ATV.dial :extSrc
@03:59:10.73 AS.reconfigure ASslow1
@03:59:10.79 AS.seek 0ml
@04:00:51.62 AFV.select :carrierSolution
@04:00:51.74 delay 2 seconds
@04:00:53.76 AS.reconfigure ASconfig
@04:00:53.82 AS.seek 0.5ml
@04:01:22.86 delay 1 second
@04:01:23.88 AFV.select :holdingCoil
@04:01:23.99 ATV.dial :extSrc
@04:01:24.01 AS.reconfigure ASslow1
@04:01:24.08 AS.seek 0ml
@04:03:04.89 ATV.dial :air2
@04:03:06.31 AS.reconfigure ASslow0
@04:03:06.37 AS.seek 0.5ml
@04:03:56.98 ATV.dial :extSrc
@04:03:58.54 AS.seek 0.02ml
@04:04:47.11 delay 1 second
@04:04:48.14 ATV.dial :waste
@04:04:49.52 AS.reconfigure ASconfig
@04:04:49.58 AS.seek 0ml
@04:04:51.03 AFV.select :holdingCoil
@04:04:51.05 ATV.dial :waste
@04:04:51.07 AS.seek 0ml
@04:04:51.16 AFV.select :carrierSolution
@04:04:51.28 delay 2 seconds
@04:04:53.30 AS.seek 0.5ml
@04:05:22.31 delay 1 second
@04:05:23.33 AFV.select :holdingCoil
@04:05:23.44 ATV.dial :air3
@04:05:24.32 AS.reconfigure ASslow1
@04:05:24.38 AS.seek 0.52ml
@04:05:28.67 ATV.dial :air3
@04:05:28.76 AS.seek 0.53ml
@04:05:31.03 delay 2 seconds
@04:05:33.06 ATV.dial :bleach
@04:05:33.53 AS.seek 0.71ml
@04:06:10.00 delay 3 seconds
@04:06:13.03 ATV.dial :air3
@04:06:13.61 delay 3 seconds
@04:06:16.68 AS.seek 0.735ml
@04:06:21.99 delay 3 seconds
@04:06:25.02 ATV.dial :bleach
@04:06:25.68 AS.seek 0.72ml
@04:06:29.11 delay 3 seconds
@04:06:32.14 ATV.dial ASV
@04:06:33.25 ASV.dial :SPErecovery
@04:06:33.34 AS.seek 0.365ml
@04:07:44.98 ATV.dial :waste
@04:07:46.82 AS.reconfigure ASnorm
@04:07:46.88 AS.seek 0ml
@04:08:05.61 ATV.dial :air3
@04:08:06.34 AS.reconfigure ASslow1
@04:08:06.40 AS.seek 0.01ml
@04:08:08.70 delay 2 seconds
@04:08:10.73 ATV.dial :bleach
@04:08:11.21 AS.seek 0.58ml
@04:10:06.09 delay 3 seconds
@04:10:09.12 ATV.dial :air3
@04:10:09.72 delay 3 seconds
@04:10:12.79 AS.seek 0.605ml
@04:10:18.11 delay 3 seconds
@04:10:21.14 ATV.dial :bleach
@04:10:21.78 AS.seek 0.59ml
@04:10:25.11 delay 3 seconds
@04:10:28.14 ATV.dial :ASV
@04:10:29.23 ASV.dial :wasteR
@04:10:29.63 AS.seek 0ml
@04:12:28.55 delay 1:00
@04:13:28.59 ATV.dial ASV
@04:13:28.60 AFV.select :carrierSolution
@04:13:28.72 delay 2 seconds
@04:13:30.74 AS.reconfigure ASconfig
@04:13:30.79 AS.seek 1ml
@04:14:28.53 delay 1 second
@04:14:29.55 AFV.select :holdingCoil
@04:14:29.66 delay 1 second
@04:14:30.68 ASV.dial :wasteR
@04:14:30.69 AS.reconfigure ASslow1
@04:14:30.77 AS.seek 0ml
@04:17:52.15 delay 10 seconds
@04:18:02.19 ATV.dial :air3
@04:18:03.79 AS.seek 0.01ml
@04:18:06.09 delay 2 seconds
@04:18:08.12 ATV.dial :bleach
@04:18:08.78 AS.seek 0.58ml
@04:20:03.67 delay 3 seconds
@04:20:06.70 ATV.dial :air3
@04:20:07.28 delay 3 seconds
@04:20:10.35 AS.seek 0.605ml
@04:20:15.66 delay 3 seconds
@04:20:18.69 ATV.dial :bleach
@04:20:19.32 AS.seek 0.59ml
@04:20:22.67 delay 3 seconds
@04:20:25.70 ATV.dial ASV
@04:20:26.80 ASV.dial :SPEdelivery
@04:20:27.20 AS.seek 0ml
@04:22:26.09 delay 1:00
@04:23:26.12 AFV.select :carrierSolution
@04:23:26.23 delay 2 seconds
@04:23:28.25 AS.reconfigure ASconfig
@04:23:28.31 AS.seek 1ml
@04:24:26.08 delay 1 second
@04:24:27.10 AFV.select :holdingCoil
@04:24:27.21 delay 1 second
@04:24:28.23 ATV.dial ASV
@04:24:28.24 ASV.dial :SPEdelivery
@04:24:28.26 AS.reconfigure ASslow1
@04:24:28.33 AS.seek 0ml
@04:27:49.69 delay 10 seconds
@04:27:59.73 ATV.dial :air2
@04:28:00.62 AS.reconfigure ASslow0
@04:28:00.67 AS.seek 0.8ml
@04:29:21.47 delay 3 seconds
@04:29:24.50 ATV.dial :ASV
@04:29:25.55 ASV.dial :SPEdelivery
@04:29:25.57 AS.reconfigure ASslow1
@04:29:25.64 AS.seek 0.03ml
@04:32:00.75 delay 20 seconds
@04:32:20.79 ATV.dial :waste,via: :air3
@04:32:22.63 AS.reconfigure ASconfig
@04:32:22.68 AS.seek 0ml
@04:32:24.72 SPE.coast
@04:32:24.80 Removing Bleach from Mixing Coil
@04:32:24.81 AFV.select :carrierSolution
@04:32:24.93 delay 2 seconds
@04:32:26.95 AS.seek 0.5ml
@04:32:55.97 delay 1 second
@04:32:56.99 AFV.select :holdingCoil
@04:32:57.10 ATV.dial :ASV
@04:32:59.48 ASV.dial :SPErecovery
@04:33:00.14 AS.reconfigure ASslow1
@04:33:00.20 AS.seek 0.73ml
@04:33:46.72 ASV.dial :wasteD
@04:33:47.32 AS.reconfigure ASnorm
@04:33:47.38 AS.seek 0ml
@04:34:24.48 AFV.select :carrierSolution
@04:34:24.60 delay 2 seconds
@04:34:26.62 AS.reconfigure ASconfig
@04:34:26.68 AS.seek 0.5ml
@04:34:55.73 delay 1 second
@04:34:56.75 AFV.select :holdingCoil
@04:34:56.86 ATV.dial ASV
@04:34:56.87 ASV.dial :SPErecovery
@04:34:57.53 AS.reconfigure ASslow0
@04:34:57.59 AS.seek 0.3ml
@04:35:17.98 ATV.dial :air2
@04:35:18.90 AS.reconfigure ASslow1
@04:35:18.96 AS.seek 0.32ml
@04:35:23.30 ATV.dial ASV
@04:35:24.40 AS.reconfigure ASslow0
@04:35:24.46 AS.seek 0.57ml
@04:35:49.94 ASV.dial :wasteD
@04:35:50.56 AS.reconfigure ASnorm
@04:35:50.62 AS.seek 0ml
@04:36:19.69 AFV.select :carrierSolution
@04:36:19.81 delay 2 seconds
@04:36:21.83 AS.reconfigure ASconfig
@04:36:21.88 AS.seek 0.5ml
@04:36:50.94 delay 1 second
@04:36:51.96 AFV.select :holdingCoil
@04:36:52.07 ATV.dial ASV
@04:36:52.08 ASV.dial :SPErecovery
@04:36:52.75 AS.reconfigure ASslow0
@04:36:52.81 AS.seek 0.3ml
@04:37:13.25 ATV.dial :air2
@04:37:14.33 AS.reconfigure ASslow1
@04:37:14.39 AS.seek 0.32ml
@04:37:18.70 ATV.dial ASV
@04:37:19.79 AS.reconfigure ASslow0
@04:37:19.85 AS.seek 0.57ml
@04:37:45.33 ASV.dial :wasteD
@04:37:45.95 AS.reconfigure ASnorm
@04:37:46.00 AS.seek 0ml
@04:38:15.08 ATV.dial :air2
@04:38:16.12 AS.reconfigure ASslow0
@04:38:16.18 AS.seek 0.3ml
@04:38:46.73 ATV.dial :ASV
@04:38:47.77 ASV.dial :wasteD
@04:38:47.79 AS.reconfigure ASconfig
@04:38:47.85 AS.seek 0.05ml
@04:39:02.55 ATV.dial :waste
@04:39:04.54 AS.seek 0ml
@04:39:07.70 ASV.dial :SPEdelivery
@04:39:08.79 Cleaning PCR
AFV.select :carrierSolution
@04:39:08.91 delay 2 seconds
@04:39:10.93 AS.seek 0.5ml
@04:39:39.98 delay 1 second
@04:39:41.00 AFV.select :holdingCoil
@04:39:41.11 ASV.dial :waste1
@04:39:41.48 ATV.dial :air3
@04:39:42.20 AS.reconfigure ASslow1
@04:39:42.26 AS.seek 0.51ml
@04:39:44.53 delay 2 seconds
@04:39:46.56 ATV.dial :bleach
@04:39:47.03 AS.seek 0.81ml
@04:40:47.70 delay 3 seconds
@04:40:50.73 ATV.dial :air3
@04:40:51.31 delay 3 seconds
@04:40:54.38 AS.seek 0.835ml
@04:40:59.73 delay 3 seconds
@04:41:02.76 ATV.dial :bleach
@04:41:03.40 AS.seek 0.82ml
@04:41:06.70 delay 3 seconds
@04:41:09.73 ARV.dial :waste
@04:41:10.36 delay 3 seconds
@04:41:13.39 ATV.dial :ARV
@04:41:14.31 AS.reconfigure ASconfig
@04:41:14.36 AS.seek 0.47ml
@04:41:34.78 AFV.select :carrierSolution
@04:41:34.90 delay 2 seconds
@04:41:36.92 AS.seek 1ml
@04:42:07.70 delay 1 second
@04:42:08.72 AFV.select :holdingCoil
@04:42:08.83 delay 1 second
@04:42:09.85 AS.reconfigure ASfast
@04:42:09.91 AS.seek 0ml
@04:42:37.27 AFV.select :carrierSolution
@04:42:37.39 delay 2 seconds
@04:42:39.41 AS.reconfigure ASconfig
@04:42:39.46 AS.seek 1ml
@04:43:37.20 delay 1 second
@04:43:38.22 AFV.select :holdingCoil
@04:43:38.33 delay 1 second
@04:43:39.35 AS.reconfigure ASfast
@04:43:39.40 AS.seek 0ml
@04:44:06.73 AFV.select :carrierSolution
@04:44:06.85 delay 2 seconds
@04:44:08.87 AS.reconfigure ASconfig
@04:44:08.92 AS.seek 1ml
@04:45:06.67 delay 1 second
@04:45:07.69 AFV.select :holdingCoil
@04:45:07.80 delay 1 second
@04:45:08.82 AS.reconfigure ASfast
@04:45:08.87 AS.seek 0ml
@04:45:36.20 ARV.dial between carrier and air1
@04:45:36.72 ATV.dial :air3
@04:45:38.08 AS.reconfigure ASslow1
@04:45:38.14 AS.seek 0.01ml
@04:45:40.48 delay 2 seconds
@04:45:42.51 ATV.dial :bleach
@04:45:43.15 AS.seek 0.81ml
@04:48:24.28 delay 3 seconds
@04:48:27.31 ATV.dial :air3
@04:48:27.89 delay 3 seconds
@04:48:30.97 AS.seek 0.835ml
@04:48:36.23 delay 3 seconds
@04:48:39.26 ATV.dial :bleach
@04:48:39.90 AS.seek 0.82ml
@04:48:43.22 delay 3 seconds
@04:48:46.25 ATV.dial PCR
@04:48:46.67 AS.reconfigure ASconfig
@04:48:46.72 AS.seek 0ml
@04:49:34.14 PCR.program :bleachProfile
@04:49:34.21 PCR program should complete in < 2:45
@04:49:34.22 PCR.run 10:00
@04:52:36.07 PCR.off
@04:52:36.11 AFV.select :carrierSolution
@04:52:36.22 delay 2 seconds
@04:52:38.24 AS.seek 1ml
@04:53:36.00 delay 1 second
@04:53:37.02 AFV.select :holdingCoil
@04:53:37.13 delay 1 second
@04:53:38.15 AS.reconfigure ASfast
@04:53:38.20 AS.seek 0ml
@04:54:05.55 delay 3 seconds
@04:54:08.57 AFV.select :carrierSolution
@04:54:08.68 delay 2 seconds
@04:54:10.70 AS.reconfigure ASconfig
@04:54:10.76 AS.seek 1ml
@04:55:08.52 delay 1 second
@04:55:09.54 AFV.select :holdingCoil
@04:55:09.65 delay 1 second
@04:55:10.67 AS.reconfigure ASfast
@04:55:10.73 AS.seek 0ml
@04:55:38.08 delay 3 seconds
@04:55:41.10 AFV.select :carrierSolution
@04:55:41.21 delay 2 seconds
@04:55:43.23 AS.reconfigure ASconfig
@04:55:43.29 AS.seek 1ml
@04:56:41.05 delay 1 second
@04:56:42.07 AFV.select :holdingCoil
@04:56:42.18 delay 1 second
@04:56:43.20 AS.reconfigure ASfast
@04:56:43.26 AS.seek 0ml
@04:57:10.61 delay 3 seconds
@04:57:13.63 AFV.select :carrierSolution
@04:57:13.74 delay 2 seconds
@04:57:15.76 AS.reconfigure ASconfig
@04:57:15.82 AS.seek 1ml
@04:58:13.56 delay 1 second
@04:58:14.58 AFV.select :holdingCoil
@04:58:14.69 delay 1 second
@04:58:15.71 AS.reconfigure ASfast
@04:58:15.77 AS.seek 0ml
@04:58:43.11 delay 3 seconds
@04:58:46.13 AFV.select :carrierSolution
@04:58:46.24 delay 2 seconds
@04:58:48.65 AS.reconfigure ASconfig
@04:58:48.74 AS.seek 1ml
@04:59:46.50 delay 1 second
@04:59:47.52 AFV.select :holdingCoil
@04:59:47.63 delay 1 second
@04:59:48.65 AS.reconfigure ASnorm
@04:59:48.71 AS.seek 0ml
@05:00:39.43 AFV.select :carrierSolution
@05:00:39.55 delay 2 seconds
@05:00:41.57 AS.reconfigure ASconfig
@05:00:41.62 AS.seek 1ml
@05:01:39.38 delay 1 second
@05:01:40.40 AFV.select :holdingCoil
@05:01:40.51 delay 1 second
@05:01:41.53 ATV.dial :waste
@05:01:42.82 AS.reconfigure ASnorm
@05:01:42.88 AS.seek 0.5ml
@05:02:08.44 ATV.dial :waste2
@05:02:09.81 AS.seek 0ml
@05:02:35.36 <mwj> Session Ended
@16:25:15.68CET21-Mar-21 <MAIN> ESP2 v3.30 I2C Gateway v3.06 for core bus
@16:25:17.79 
Configuration for mer 7/20/20 brent@mbari.org
/home/esp/esp2/type/shallow/mer/configure.rb
@16:25:17.88 
Configuration for MFB3 by preston@mbari.org
/home/esp/esp2/type/mfb/MFB3/configure.rb
@16:25:22.30 Polling Can every 10:00
@16:25:24.01 CS.configure :default
@16:25:24.05 CS.hold
@16:25:24.09 CS.configure :default
@16:25:24.17 PS.configure :default
@16:25:24.25 SS.configure :default
@16:25:24.29 SS.hold
@16:25:24.33 SS.configure :default
@16:25:24.41 CC.configure :default
@16:25:24.45 CC.hold
@16:25:24.50 CC.configure :default
@16:25:24.58 PC.configure :default
@16:25:24.62 PC.hold
@16:25:24.68 PC.configure :default
@16:25:24.78 Forearm.configure :loose
@16:25:24.82 Forearm.hold
@16:25:24.87 Forearm.configure :loose
@16:25:24.95 Elbow.configure :loose
@16:25:24.99 Elbow.hold
@16:25:25.04 Elbow.configure :loose
@16:25:25.14 SC.configure :loose
@16:25:25.18 SC.hold
@16:25:25.22 SC.configure :loose
@16:25:25.30 SE.configure :default
@16:25:25.34 SE.hold
@16:25:25.39 SE.configure :default
@16:25:25.43 CH.coast
@16:25:25.47 CH.configure :default
@16:25:25.50 PH.coast
@16:25:25.55 PH.configure :default
@16:25:25.65 SPE.coast
@16:25:25.69 SPE.configure SPEconfig
@16:25:25.77 AS.configure ASconfig
@16:25:25.80 AS.hold
@16:25:25.86 AS.configure ASconfig
@16:25:25.91 PCR.off
@16:25:25.94 PCR.pidtemp= 50,0.1,0,300,25,25
@16:25:25.97 PCR.caltemp= 2.5051,0.0372,0
@16:25:26.00 All dwarf controllers running firmware version 3.75
@16:25:26.17 Cleanly exited at 16:24:58CET21-Mar-21
@16:25:26.20 Loading /home/esp/esp2/mission/GENOMAP.rb mission
@16:25:26.34 <MFBPCR> Extracting Nucleic Acid from extSrc
@16:25:26.37 SPE.hold 2:46:40,55C
@16:25:26.40 ASV.dial :SPEdelivery
@16:25:26.84 delay 20 seconds
@16:25:29.73 <mwj> Session Begun
@16:25:46.87 <MFBPCR> AFV.select :holdingCoil
@16:25:46.99 ATV.dial :air2
@16:25:48.28 AS.reconfigure ASslow1
@16:25:48.34 AS.seek 0.07ml
@16:26:02.70 delay 3 seconds
@16:26:05.73 ATV.dial :extSrc
@16:26:07.23 AS.seek 0.47ml
@16:27:27.92 delay 5 seconds
@16:27:33.00 Solid Phase Extraction Heater at 38.3C
@16:27:33.01 ATV.dial ASV
@16:27:33.91 ASV.dial :SPEdelivery
@16:27:34.00 AS.seek 0.042ml
@16:29:00.36 delay 10 seconds
@16:29:10.40 Rinsing external lysate line extSrc
@16:29:10.41 AFV.select :carrierSolution
@16:29:10.53 delay 2 seconds
@16:29:12.55 AS.reconfigure ASconfig
@16:29:12.61 AS.seek 1ml
@16:30:07.98 delay 1 second
@16:30:09.00 AFV.select :holdingCoil
@16:30:09.11 delay 1 second
@16:30:10.13 ATV.dial :extSrc
@16:30:10.96 AS.reconfigure ASnorm
@16:30:11.01 AS.seek 0ml
@16:31:01.70 ATV.dial :air2
@16:31:03.29 AS.reconfigure ASslow0
@16:31:03.35 AS.seek 0.05ml
@16:31:08.72 ATV.dial :extSrc
@16:31:10.31 AS.seek 0.01ml
@16:31:14.62 ATV.dial :waste
@16:31:15.99 AS.reconfigure ASconfig
@16:31:16.04 AS.seek 0ml
@16:31:16.89 ethanol wash
@16:31:17.28 ATV.dial :air2
@16:31:18.45 AS.reconfigure ASslow2
@16:31:18.51 AS.seek 0.01ml
@16:31:28.89 delay 3 seconds
@16:31:31.92 Pulling 0.4ml of Ethanol Wash
@16:31:31.93 ATV.dial :ethanol
@16:31:32.81 AS.reconfigure ASslow1
@16:31:32.87 AS.seek 0.41ml
@16:32:53.56 delay 3 seconds
@16:32:56.59 ATV.dial :air1
@16:32:57.01 delay 3 seconds
@16:33:00.08 AS.seek 0.435ml
@16:33:05.40 delay 3 seconds
@16:33:08.43 ATV.dial :ethanol
@16:33:09.04 AS.seek 0.42ml
@16:33:12.44 delay 3 seconds
@16:33:15.47 ATV.dial ASV
@16:33:16.87 AS.seek 0.093ml
@16:34:22.98 delay 3 seconds
@16:34:26.01 ATV.dial :waste2
@16:34:26.81 AS.reconfigure ASconfig
@16:34:26.86 AS.seek 0ml
@16:34:32.51 ATV.dial :air2
@16:34:32.93 AS.reconfigure ASslow1
@16:34:32.98 AS.seek 1ml
@16:37:54.34 delay 5 seconds
@16:37:59.37 ATV.dial ASV
@16:38:00.39 ASV.dial :SPEdelivery
@16:38:00.41 AS.seek 0.02ml
@16:41:17.81 delay 5 seconds
@16:41:22.84 ATV.dial :air2
@16:41:23.93 AS.seek 0.52ml
@16:43:04.69 delay 5 seconds
@16:43:09.72 ATV.dial ASV
@16:43:10.75 ASV.dial :wasteR
@16:43:11.23 AS.seek 0.3ml
@16:43:55.75 delay 20 seconds
@16:44:15.79 AS.seek 0.07ml
@16:45:02.33 delay 20 seconds
@16:45:22.36 ATV.dial :waste2
@16:45:23.17 AS.reconfigure ASconfig
@16:45:23.22 AS.seek 0ml
@16:45:27.53 Elution
@16:45:27.54 ATV.dial :air1
@16:45:28.73 AS.reconfigure ASslow1
@16:45:28.78 AS.seek 0.05ml
@16:45:39.16 delay 3 seconds
@16:45:42.19 ATV.dial :ElutionWater
@16:45:42.90 AS.seek 0.11ml
@16:45:55.30 delay 3 seconds
@16:45:58.33 ATV.dial ASV,via: :air2
@16:46:00.54 ASV.dial :SPEelution
@16:46:01.16 AFV.select :carrierSolution
@16:46:01.32 AS.reconfigure ASnorm
@16:46:01.38 AS.seek 0.76ml
@16:46:34.48 delay 3 seconds
@16:46:37.50 AFV.select :holdingCoil
@16:46:37.65 AS.reconfigure ASslow1
@16:46:37.71 AS.seek 0.475ml
@16:47:35.30 delay 5 seconds
@16:47:40.38 AS.reconfigure ASslow2
@16:47:40.44 AS.seek 0.44ml
@16:48:16.25 delay 1:00
@16:49:16.29 pushing eluate to holding coil
@16:49:16.36 AS.seek 0.28ml
@16:51:57.77 delay 30 seconds
@16:52:27.81 ATV.dial :waste,via: :air3
@16:52:29.65 AS.reconfigure ASconfig
@16:52:29.70 AS.seek 0ml
@16:52:46.11 Parking Bleach in LysateSource line and SPEcolumn
@16:52:46.12 ATV.dial :air3
@16:52:47.03 AS.reconfigure ASslow1
@16:52:47.08 AS.seek 0.03ml
@16:52:53.42 ATV.dial :ElutionWater
@16:52:54.06 AS.seek 0.06ml
@16:53:00.37 ATV.dial :air3
@16:53:01.01 AS.seek 0.07ml
@16:53:03.41 delay 2 seconds
@16:53:05.44 ATV.dial :bleach
@16:53:05.93 AS.seek 0.15ml
@16:53:22.30 delay 3 seconds
@16:53:25.33 ATV.dial :air3
@16:53:25.91 delay 3 seconds
@16:53:28.98 AS.seek 0.175ml
@16:53:34.27 delay 3 seconds
@16:53:37.30 ATV.dial :bleach
@16:53:37.93 AS.seek 0.16ml
@16:53:41.22 delay 3 seconds
@16:53:44.25 ATV.dial :extSrc
@16:53:44.90 AS.reconfigure ASconfig
@16:53:44.95 AS.seek 0.015ml
@16:53:53.59 ATV.dial :waste
@16:53:55.13 AS.seek 0ml
@16:53:56.31 ATV.dial :air3
@16:53:57.20 AS.reconfigure ASslow1
@16:53:57.25 AS.seek 0.01ml
@16:53:59.66 delay 2 seconds
@16:54:01.69 ATV.dial :bleach
@16:54:02.17 AS.seek 0.58ml
@16:55:57.06 delay 3 seconds
@16:56:00.09 ATV.dial :air3
@16:56:00.67 delay 3 seconds
@16:56:03.74 AS.seek 0.605ml
@16:56:09.04 delay 3 seconds
@16:56:12.07 ATV.dial :bleach
@16:56:12.72 AS.seek 0.59ml
@16:56:16.03 delay 3 seconds
@16:56:19.06 ATV.dial :ASV
@16:56:20.16 ASV.dial :wasteR
@16:56:20.79 AS.seek 0ml
@16:58:19.70 delay 1:00
@16:59:19.73 AFV.select :carrierSolution
@16:59:19.84 delay 2 seconds
@16:59:21.86 AS.reconfigure ASconfig
@16:59:21.91 AS.seek 1ml
@17:00:19.69 delay 1 second
@17:00:20.71 AFV.select :holdingCoil
@17:00:20.83 delay 1 second
@17:00:21.85 ATV.dial ASV
@17:00:21.86 ASV.dial :wasteR
@17:00:21.88 AS.reconfigure ASslow1
@17:00:21.95 AS.seek 0ml
@17:03:43.36 delay 20 seconds
@17:04:03.40 ATV.dial :air3
@17:04:05.00 AS.seek 0.59ml
@17:06:03.92 delay 3 seconds
@17:06:06.95 ATV.dial :ASV
@17:06:08.45 ASV.dial :wasteR
@17:06:08.53 AS.seek 0.09ml
@17:07:49.39 delay 30 seconds
@17:08:19.43 ATV.dial :extSrc
@17:08:20.23 delay 3 seconds
@17:08:23.26 ATV.dial :ElutionWater
@17:08:24.45 AS.seek 0.12ml
@17:08:30.88 ATV.dial :air3
@17:08:31.53 AS.seek 0.13ml
@17:08:33.81 delay 2 seconds
@17:08:35.84 ATV.dial :bleach
@17:08:36.31 AS.seek 0.23ml
@17:08:56.69 delay 3 seconds
@17:08:59.72 ATV.dial :air3
@17:09:00.30 delay 3 seconds
@17:09:03.37 AS.seek 0.255ml
@17:09:08.64 delay 3 seconds
@17:09:11.67 ATV.dial :bleach
@17:09:12.31 AS.seek 0.24ml
@17:09:15.94 delay 3 seconds
@17:09:19.05 ATV.dial :ASV
@17:09:20.16 ASV.dial :SPEdelivery
@17:09:20.56 AFV.select :carrierSolution
@17:09:20.69 delay 2 seconds
@17:09:22.71 AS.reconfigure ASconfig
@17:09:22.76 AS.seek 0.5ml
@17:09:37.98 delay 1 second
@17:09:39.00 AFV.select :holdingCoil
@17:09:39.15 AS.reconfigure ASslow1
@17:09:39.21 AS.seek 0.17ml
@17:10:45.87 SPE.hold 2:46:40,95C
@17:10:45.93 delay 3:00
@17:13:45.97 AFV.select :carrierSolution
@17:13:46.09 delay 2 seconds
@17:13:48.11 AS.reconfigure ASconfig
@17:13:48.16 AS.seek 1ml
@17:14:36.14 delay 1 second
@17:14:37.16 AFV.select :holdingCoil
@17:14:37.27 delay 1 second
@17:14:38.29 ASV.dial :wasteD
@17:14:39.40 AS.seek 0ml
@17:15:37.19 Priming Eluate
@17:15:37.20 AFV.select :carrierSolution
@17:15:37.33 delay 2 seconds
@17:15:39.35 AS.seek 0.5ml
@17:16:08.38 delay 1 second
@17:16:09.40 AFV.select :holdingCoil
@17:16:09.51 ATV.dial ASV
@17:16:09.52 ASV.dial :SPErecovery
@17:16:10.16 Swish #1 of 2
@17:16:10.24 AS.reconfigure ASslow0
@17:16:10.30 AS.seek 0.6ml
@17:16:20.76 AS.seek 0.5ml
@17:16:31.17 Swish #2 of 2
@17:16:31.25 AS.seek 0.6ml
@17:16:41.75 AS.seek 0.5ml
@17:16:52.21 delay 3 seconds
@17:16:55.29 AS.reconfigure ASslow1
@17:16:55.35 AS.seek 0.684ml
@17:17:32.64 delay 3 seconds
@17:17:35.67 Eluate primed
@17:17:35.68 ATV.dial :waste2
@17:17:36.35 AS.reconfigure ASnorm
@17:17:36.40 AS.seek 0ml
@17:18:11.20 SPE.coast
@17:18:11.28 ASV.dial :SPErecovery
@17:18:11.30 Setting up PCR for ACYPP2 -- writing /var/log/esp/ACYPP221mar2117h.pcr
@17:18:11.33 Building PCR Slug from ASV with MM1 mix, PP2 primer
@17:18:11.35 Priming with carrier
@17:18:11.37 ATV.dial ARV
@17:18:12.41 ARV.dial :carrier
@17:18:12.76 AS.seek 0.5ml
@17:18:38.33 AFV.select :carrierSolution
@17:18:38.46 delay 2 seconds
@17:18:40.50 AS.reconfigure ASconfig
@17:18:40.57 AS.seek 1ml
@17:19:09.59 delay 1 second
@17:19:10.62 AFV.select :holdingCoil
@17:19:10.72 delay 1 second
@17:19:11.74 ATV.dial :waste
@17:19:13.37 AS.reconfigure ASnorm
@17:19:13.43 AS.seek 0ml
@17:20:04.13 AFV.select :carrierSolution
@17:20:04.26 delay 2 seconds
@17:20:06.28 AS.reconfigure ASconfig
@17:20:06.34 AS.seek 0.5ml
@17:20:35.36 delay 1 second
@17:20:36.38 AFV.select :holdingCoil
@17:20:36.49 Building Slug
@17:20:36.50 ATV.dial :ARV
@17:20:38.29 delay 2 seconds
@17:20:40.31 ARV.dial :air1
@17:20:40.71 delay 2 seconds
@17:20:42.79 AS.reconfigure ASslow2
@17:20:42.84 AS.seek 0.506ml
@17:20:49.17 delay 2 seconds
@17:20:51.20 ARV.dial :MM1
@17:20:51.83 delay 2 seconds
@17:20:53.86 Adding MM1
@17:20:53.93 AS.seek 0.524ml
@17:21:12.42 delay 25 seconds
@17:21:37.45 ARV.dial :PP2
@17:21:37.87 Adding PP2
@17:21:37.88 delay 2 seconds
@17:21:39.96 AS.seek 0.53ml
@17:21:46.31 delay 10 seconds
@17:21:56.35 ARV.dial :air2
@17:21:56.78 adding ARV air
@17:21:56.79 delay 2 seconds
@17:21:58.87 AS.seek 0.536ml
@17:22:05.22 delay 5 seconds
@17:22:10.25 ARV.dial :carrier
@17:22:11.59 delay 7 seconds
@17:22:18.68 AS.seek 0.594ml
@17:23:17.42 delay 5 seconds
@17:23:22.45 ATV.dial :ASV
@17:23:22.86 delay 2 seconds
@17:23:24.94 AS.seek 0.6ml
@17:23:31.20 delay 4 seconds
@17:23:35.23 ATV.dial ARV
@17:23:35.81 delay 2 seconds
@17:23:37.84 ARV.dial :carrier
@17:23:37.85 delay 2 seconds
@17:23:39.93 AS.seek 0.65ml
@17:24:30.44 delay 4 seconds
@17:24:34.47 ATV.dial PCR
@17:24:35.11 ASV.dial :waste1
@17:24:35.93 ARV.dial :air1
@17:24:36.35 Positioning Slug by 0.324ml
@17:24:36.44 AS.seek 0.326ml
@17:30:02.47 delay 5 seconds
@17:30:07.50 ATV.dial ARV
@17:30:08.31 ASV.dial :PCRlock
@17:30:08.70 delay 5 seconds
@17:30:13.73 Running ACYPP2 Profile60CIQ2 thermocycling
@17:30:13.74 <PCR> PCR.program Profile60CIQ2
@17:30:13.86 Profile60CIQ2 should complete in < 1:47:50
@17:30:13.87 PCR.run 2:19:47.5
@18:58:08.90 PCR.off
@18:58:09.52 <MFBPCR> Running ACYPP2 MeltProfile60 thermocycling
@18:58:09.53 <PCR> PCR.program MeltProfile60
@18:58:09.64 MeltProfile60 should complete in < 11:55
@18:58:09.65 PCR.run 19:53.75
@19:05:32.51 PCR.off
@19:05:33.45 <MFBPCR> PCR for ACYPP2 completed -- closed /var/log/esp/ACYPP221mar2117h.pcr
@19:05:33.52 Cleaning PCR
@19:05:33.53 AFV.select :carrierSolution
@19:05:33.65 delay 2 seconds
@19:05:35.67 AS.reconfigure ASconfig
@19:05:35.72 AS.seek 0.5ml
@19:05:46.02 delay 1 second
@19:05:47.04 AFV.select :holdingCoil
@19:05:47.15 ASV.dial :waste1
@19:05:47.55 ATV.dial :air3
@19:05:48.92 AS.reconfigure ASslow1
@19:05:48.98 AS.seek 0.51ml
@19:05:51.33 delay 2 seconds
@19:05:53.36 ATV.dial :bleach
@19:05:54.02 AS.seek 0.81ml
@19:06:54.64 delay 3 seconds
@19:06:57.67 ATV.dial :air3
@19:06:58.25 delay 3 seconds
@19:07:01.32 AS.seek 0.835ml
@19:07:06.61 delay 3 seconds
@19:07:09.64 ATV.dial :bleach
@19:07:10.28 AS.seek 0.82ml
@19:07:13.58 delay 3 seconds
@19:07:16.61 ARV.dial :waste
@19:07:17.25 delay 3 seconds
@19:07:20.28 ATV.dial :ARV
@19:07:21.21 AS.reconfigure ASconfig
@19:07:21.27 AS.seek 0.47ml
@19:07:41.68 AFV.select :carrierSolution
@19:07:41.80 delay 2 seconds
@19:07:43.82 AS.seek 1ml
@19:08:14.61 delay 1 second
@19:08:15.63 AFV.select :holdingCoil
@19:08:15.74 delay 1 second
@19:08:16.76 AS.reconfigure ASfast
@19:08:16.81 AS.seek 0ml
@19:08:44.13 AFV.select :carrierSolution
@19:08:44.25 delay 2 seconds
@19:08:46.27 AS.reconfigure ASconfig
@19:08:46.32 AS.seek 1ml
@19:09:44.08 delay 1 second
@19:09:45.10 AFV.select :holdingCoil
@19:09:45.21 delay 1 second
@19:09:46.23 AS.reconfigure ASfast
@19:09:46.28 AS.seek 0ml
@19:10:13.61 AFV.select :carrierSolution
@19:10:13.72 delay 2 seconds
@19:10:15.74 AS.reconfigure ASconfig
@19:10:15.79 AS.seek 1ml
@19:11:13.55 delay 1 second
@19:11:14.57 AFV.select :holdingCoil
@19:11:14.68 delay 1 second
@19:11:15.70 AS.reconfigure ASfast
@19:11:15.75 AS.seek 0ml
@19:11:43.08 ARV.dial between carrier and air1
@19:11:43.61 ATV.dial :air3
@19:11:44.96 AS.reconfigure ASslow1
@19:11:45.02 AS.seek 0.01ml
@19:11:47.33 delay 2 seconds
@19:11:49.36 ATV.dial :bleach
@19:11:50.00 AS.seek 0.81ml
@19:14:31.13 delay 3 seconds
@19:14:34.16 ATV.dial :air3
@19:14:34.74 delay 3 seconds
@19:14:37.81 AS.seek 0.835ml
@19:14:43.08 delay 3 seconds
@19:14:46.11 ATV.dial :bleach
@19:14:46.75 AS.seek 0.82ml
@19:14:50.05 delay 3 seconds
@19:14:53.08 ATV.dial PCR
@19:14:53.50 AS.reconfigure ASconfig
@19:14:53.55 AS.seek 0ml
@19:15:40.96 PCR.program :bleachProfile
@19:15:41.03 PCR program should complete in < 2:45
@19:15:41.04 PCR.run 10:00
@19:18:40.26 PCR.off
@19:18:40.31 AFV.select :carrierSolution
@19:18:40.42 delay 2 seconds
@19:18:42.44 AS.seek 1ml
@19:19:40.22 delay 1 second
@19:19:41.24 AFV.select :holdingCoil
@19:19:41.35 delay 1 second
@19:19:42.37 AS.reconfigure ASfast
@19:19:42.43 AS.seek 0ml
@19:20:09.75 delay 3 seconds
@19:20:12.77 AFV.select :carrierSolution
@19:20:12.88 delay 2 seconds
@19:20:14.90 AS.reconfigure ASconfig
@19:20:14.96 AS.seek 1ml
@19:21:12.72 delay 1 second
@19:21:13.74 AFV.select :holdingCoil
@19:21:13.85 delay 1 second
@19:21:14.87 AS.reconfigure ASfast
@19:21:14.93 AS.seek 0ml
@19:21:42.29 delay 3 seconds
@19:21:45.31 AFV.select :carrierSolution
@19:21:45.42 delay 2 seconds
@19:21:47.45 AS.reconfigure ASconfig
@19:21:47.50 AS.seek 1ml
@19:22:45.25 delay 1 second
@19:22:46.27 AFV.select :holdingCoil
@19:22:46.38 delay 1 second
@19:22:47.40 AS.reconfigure ASfast
@19:22:47.46 AS.seek 0ml
@19:23:14.79 delay 3 seconds
@19:23:17.81 AFV.select :carrierSolution
@19:23:17.92 delay 2 seconds
@19:23:19.94 AS.reconfigure ASconfig
@19:23:20.00 AS.seek 1ml
@19:24:17.80 delay 1 second
@19:24:18.82 AFV.select :holdingCoil
@19:24:18.93 delay 1 second
@19:24:19.95 AS.reconfigure ASfast
@19:24:20.01 AS.seek 0ml
@19:24:47.33 delay 3 seconds
@19:24:50.35 AFV.select :carrierSolution
@19:24:50.46 delay 2 seconds
@19:24:52.48 AS.reconfigure ASconfig
@19:24:52.53 AS.seek 1ml
@19:25:50.29 delay 1 second
@19:25:51.31 AFV.select :holdingCoil
@19:25:51.42 delay 1 second
@19:25:52.44 AS.reconfigure ASnorm
@19:25:52.49 AS.seek 0ml
@19:26:43.24 Setting up PCR for AOPP1 -- writing /var/log/esp/AOPP121mar2119h.pcr
@19:26:43.25 Building PCR Slug from ASV with MM1 mix, PP1 primer
@19:26:43.27 Priming with carrier
@19:26:43.29 ATV.dial ARV
@19:26:43.96 ARV.dial :carrier
@19:26:44.32 AS.seek 0.5ml
@19:27:09.86 AFV.select :carrierSolution
@19:27:09.99 delay 2 seconds
@19:27:12.01 AS.reconfigure ASconfig
@19:27:12.07 AS.seek 1ml
@19:27:41.10 delay 1 second
@19:27:42.12 AFV.select :holdingCoil
@19:27:42.23 delay 1 second
@19:27:43.25 ATV.dial :waste
@19:27:45.03 AS.reconfigure ASnorm
@19:27:45.09 AS.seek 0ml
@19:28:35.80 AFV.select :carrierSolution
@19:28:35.92 delay 2 seconds
@19:28:37.94 AS.reconfigure ASconfig
@19:28:38.00 AS.seek 0.5ml
@19:29:07.04 delay 1 second
@19:29:08.06 AFV.select :holdingCoil
@19:29:08.17 Building Slug
@19:29:08.18 ATV.dial :ARV
@19:29:09.93 delay 2 seconds
@19:29:11.96 ARV.dial :air1
@19:29:12.36 delay 2 seconds
@19:29:14.43 AS.reconfigure ASslow2
@19:29:14.49 AS.seek 0.506ml
@19:29:20.75 delay 2 seconds
@19:29:22.78 ARV.dial :MM1
@19:29:23.41 delay 2 seconds
@19:29:25.44 Adding MM1
@19:29:25.51 AS.seek 0.524ml
@19:29:43.96 delay 25 seconds
@19:30:08.99 ARV.dial :PP1
@19:30:09.39 Adding PP1
@19:30:09.40 delay 2 seconds
@19:30:11.48 AS.seek 0.53ml
@19:30:17.85 delay 10 seconds
@19:30:27.89 ARV.dial :air1
@19:30:28.31 adding ARV air
@19:30:28.32 delay 2 seconds
@19:30:30.39 AS.seek 0.536ml
@19:30:36.74 delay 5 seconds
@19:30:41.77 ARV.dial :carrier
@19:30:42.19 delay 7 seconds
@19:30:49.27 AS.seek 0.594ml
@19:31:47.80 delay 5 seconds
@19:31:52.83 ATV.dial :ASV
@19:31:54.01 delay 2 seconds
@19:31:56.08 AS.seek 0.6ml
@19:32:02.40 delay 4 seconds
@19:32:06.43 ATV.dial ARV
@19:32:07.11 delay 2 seconds
@19:32:09.14 ARV.dial :carrier
@19:32:09.15 delay 2 seconds
@19:32:11.23 AS.seek 0.65ml
@19:33:02.24 delay 4 seconds
@19:33:06.27 ATV.dial PCR
@19:33:06.91 ASV.dial :waste1
@19:33:06.93 ARV.dial :air1
@19:33:07.35 Positioning Slug by 0.324ml
@19:33:07.43 AS.seek 0.326ml
@19:38:33.58 delay 5 seconds
@19:38:38.61 ATV.dial ARV
@19:38:39.52 ASV.dial :PCRlock
@19:38:39.93 delay 5 seconds
@19:38:44.96 Running AOPP1 Profile60CIQ1 thermocycling
@19:38:44.97 <PCR> PCR.program Profile60CIQ1
@19:38:45.09 Profile60CIQ1 should complete in < 1:56:15
@19:38:45.10 PCR.run 2:30:18.75
@21:17:05.41 PCR.off
@21:17:05.98 <MFBPCR> Running AOPP1 MeltProfile60 thermocycling
@21:17:06.00 <PCR> PCR.program MeltProfile60
@21:17:06.09 MeltProfile60 should complete in < 11:55
@21:17:06.10 PCR.run 19:53.75
@21:24:25.37 PCR.off
@21:24:26.29 <MFBPCR> PCR for AOPP1 completed -- closed /var/log/esp/AOPP121mar2119h.pcr
@21:24:26.36 Cleaning PCR
@21:24:26.37 AFV.select :carrierSolution
@21:24:26.49 delay 2 seconds
@21:24:28.51 AS.reconfigure ASconfig
@21:24:28.56 AS.seek 0.5ml
@21:24:38.92 delay 1 second
@21:24:39.94 AFV.select :holdingCoil
@21:24:40.05 ASV.dial :waste1
@21:24:40.45 ATV.dial :air3
@21:24:41.82 AS.reconfigure ASslow1
@21:24:41.87 AS.seek 0.51ml
@21:24:44.15 delay 2 seconds
@21:24:46.18 ATV.dial :bleach
@21:24:46.84 AS.seek 0.81ml
@21:25:47.54 delay 3 seconds
@21:25:50.57 ATV.dial :air3
@21:25:51.54 delay 3 seconds
@21:25:54.61 AS.seek 0.835ml
@21:25:59.96 delay 3 seconds
@21:26:02.99 ATV.dial :bleach
@21:26:03.63 AS.seek 0.82ml
@21:26:06.96 delay 3 seconds
@21:26:09.99 ARV.dial :waste
@21:26:10.62 delay 3 seconds
@21:26:13.65 ATV.dial :ARV
@21:26:14.68 AS.reconfigure ASconfig
@21:26:14.74 AS.seek 0.47ml
@21:26:35.15 AFV.select :carrierSolution
@21:26:35.27 delay 2 seconds
@21:26:37.29 AS.seek 1ml
@21:27:08.06 delay 1 second
@21:27:09.08 AFV.select :holdingCoil
@21:27:09.19 delay 1 second
@21:27:10.21 AS.reconfigure ASfast
@21:27:10.27 AS.seek 0ml
@21:27:37.62 AFV.select :carrierSolution
@21:27:37.74 delay 2 seconds
@21:27:39.76 AS.reconfigure ASconfig
@21:27:39.81 AS.seek 1ml
@21:28:37.57 delay 1 second
@21:28:38.59 AFV.select :holdingCoil
@21:28:38.70 delay 1 second
@21:28:39.72 AS.reconfigure ASfast
@21:28:39.78 AS.seek 0ml
@21:29:07.12 AFV.select :carrierSolution
@21:29:07.24 delay 2 seconds
@21:29:09.26 AS.reconfigure ASconfig
@21:29:09.31 AS.seek 1ml
@21:30:07.09 delay 1 second
@21:30:08.11 AFV.select :holdingCoil
@21:30:08.22 delay 1 second
@21:30:09.24 AS.reconfigure ASfast
@21:30:09.30 AS.seek 0ml
@21:30:36.65 ARV.dial between carrier and air1
@21:30:37.17 ATV.dial :air3
@21:30:38.54 AS.reconfigure ASslow1
@21:30:38.60 AS.seek 0.01ml
@21:30:40.90 delay 2 seconds
@21:30:42.93 ATV.dial :bleach
@21:30:43.57 AS.seek 0.81ml
@21:33:24.74 delay 3 seconds
@21:33:27.77 ATV.dial :air3
@21:33:28.35 delay 3 seconds
@21:33:31.42 AS.seek 0.835ml
@21:33:36.77 delay 3 seconds
@21:33:39.80 ATV.dial :bleach
@21:33:40.45 AS.seek 0.82ml
@21:33:43.73 delay 3 seconds
@21:33:46.76 ATV.dial PCR
@21:33:47.19 AS.reconfigure ASconfig
@21:33:47.24 AS.seek 0ml
@21:34:34.65 PCR.program :bleachProfile
@21:34:34.73 PCR program should complete in < 2:45
@21:34:34.74 PCR.run 10:00
@21:37:33.91 PCR.off
@21:37:34.02 AFV.select :carrierSolution
@21:37:34.13 delay 2 seconds
@21:37:36.15 AS.seek 1ml
@21:38:33.90 delay 1 second
@21:38:34.92 AFV.select :holdingCoil
@21:38:35.03 delay 1 second
@21:38:36.05 AS.reconfigure ASfast
@21:38:36.11 AS.seek 0ml
@21:39:03.46 delay 3 seconds
@21:39:06.48 AFV.select :carrierSolution
@21:39:06.59 delay 2 seconds
@21:39:08.61 AS.reconfigure ASconfig
@21:39:08.68 AS.seek 1ml
@21:40:06.42 delay 1 second
@21:40:07.44 AFV.select :holdingCoil
@21:40:07.55 delay 1 second
@21:40:08.57 AS.reconfigure ASfast
@21:40:08.63 AS.seek 0ml
@21:40:35.96 delay 3 seconds
@21:40:38.99 AFV.select :carrierSolution
@21:40:39.09 delay 2 seconds
@21:40:41.11 AS.reconfigure ASconfig
@21:40:41.16 AS.seek 1ml
@21:41:38.95 delay 1 second
@21:41:39.97 AFV.select :holdingCoil
@21:41:40.08 delay 1 second
@21:41:41.10 AS.reconfigure ASfast
@21:41:41.16 AS.seek 0ml
@21:42:08.51 delay 3 seconds
@21:42:11.53 AFV.select :carrierSolution
@21:42:11.64 delay 2 seconds
@21:42:13.66 AS.reconfigure ASconfig
@21:42:13.73 AS.seek 1ml
@21:43:11.48 delay 1 second
@21:43:12.50 AFV.select :holdingCoil
@21:43:12.61 delay 1 second
@21:43:13.63 AS.reconfigure ASfast
@21:43:13.69 AS.seek 0ml
@21:43:41.04 delay 3 seconds
@21:43:44.06 AFV.select :carrierSolution
@21:43:44.17 delay 2 seconds
@21:43:46.19 AS.reconfigure ASconfig
@21:43:46.25 AS.seek 1ml
@21:44:44.04 delay 1 second
@21:44:45.06 AFV.select :holdingCoil
@21:44:45.17 delay 1 second
@21:44:46.19 AS.reconfigure ASnorm
@21:44:46.24 AS.seek 0ml
@21:45:36.96 Setting up PCR for AARPP4 -- writing /var/log/esp/AARPP421mar2121h.pcr
@21:45:36.97 Building PCR Slug from ASV with MM2 mix, PP4 primer
@21:45:37.00 Priming with carrier
@21:45:37.01 ATV.dial ARV
@21:45:37.65 ARV.dial :carrier
@21:45:37.99 AS.seek 0.5ml
@21:46:03.54 AFV.select :carrierSolution
@21:46:03.66 delay 2 seconds
@21:46:05.68 AS.reconfigure ASconfig
@21:46:05.75 AS.seek 1ml
@21:46:34.79 delay 1 second
@21:46:35.81 AFV.select :holdingCoil
@21:46:35.92 delay 1 second
@21:46:36.94 ATV.dial :waste
@21:46:38.72 AS.reconfigure ASnorm
@21:46:38.78 AS.seek 0ml
@21:47:29.45 AFV.select :carrierSolution
@21:47:29.58 delay 2 seconds
@21:47:31.60 AS.reconfigure ASconfig
@21:47:31.66 AS.seek 0.5ml
@21:48:00.73 delay 1 second
@21:48:01.75 AFV.select :holdingCoil
@21:48:01.86 Building Slug
@21:48:01.87 ATV.dial :ARV
@21:48:03.73 delay 2 seconds
@21:48:05.76 ARV.dial :air1
@21:48:06.17 delay 2 seconds
@21:48:08.24 AS.reconfigure ASslow2
@21:48:08.30 AS.seek 0.506ml
@21:48:14.85 delay 2 seconds
@21:48:16.88 ARV.dial :MM2
@21:48:18.45 delay 2 seconds
@21:48:20.48 Adding MM2
@21:48:20.55 AS.seek 0.524ml
@21:48:39.39 delay 25 seconds
@21:49:04.42 ARV.dial :PP4
@21:49:04.83 Adding PP4
@21:49:04.84 delay 2 seconds
@21:49:06.91 AS.seek 0.53ml
@21:49:13.17 delay 10 seconds
@21:49:23.21 ARV.dial :air3
@21:49:23.61 adding ARV air
@21:49:23.62 delay 2 seconds
@21:49:25.69 AS.seek 0.536ml
@21:49:31.89 delay 5 seconds
@21:49:36.92 ARV.dial :carrier
@21:49:38.23 delay 7 seconds
@21:49:45.30 AS.seek 0.594ml
@21:50:43.87 delay 5 seconds
@21:50:48.90 ATV.dial :ASV
@21:50:49.32 delay 2 seconds
@21:50:51.39 AS.seek 0.6ml
@21:50:57.76 delay 4 seconds
@21:51:01.79 ATV.dial ARV
@21:51:02.37 delay 2 seconds
@21:51:04.40 ARV.dial :carrier
@21:51:04.41 delay 2 seconds
@21:51:06.48 AS.seek 0.65ml
@21:51:57.04 delay 4 seconds
@21:52:01.07 ATV.dial PCR
@21:52:01.71 ASV.dial :waste1
@21:52:01.73 ARV.dial :air1
@21:52:02.15 Positioning Slug by 0.324ml
@21:52:02.24 AS.seek 0.326ml
@21:57:28.42 delay 5 seconds
@21:57:33.45 ATV.dial ARV
@21:57:34.26 ASV.dial :PCRlock
@21:57:34.67 delay 5 seconds
@21:57:39.70 Running AARPP4 Profile58CIQ2 thermocycling
@21:57:39.71 <PCR> PCR.program Profile58CIQ2
@21:57:39.84 Profile58CIQ2 should complete in < 1:36:15
@21:57:39.85 PCR.run 2:05:18.75
@23:17:44.64 PCR.off
@23:17:45.20 <MFBPCR> Running AARPP4 MeltProfile58 thermocycling
@23:17:45.21 <PCR> PCR.program MeltProfile58
@23:17:45.32 MeltProfile58 should complete in < 12:33
@23:17:45.33 PCR.run 20:41.25
@23:25:14.84 PCR.off
@23:25:16.20 <MFBPCR> PCR for AARPP4 completed -- closed /var/log/esp/AARPP421mar2121h.pcr
@23:25:16.27 Cleaning PCR
@23:25:16.28 AFV.select :carrierSolution
@23:25:16.40 delay 2 seconds
@23:25:18.42 AS.reconfigure ASconfig
@23:25:18.47 AS.seek 0.5ml
@23:25:28.81 delay 1 second
@23:25:29.82 AFV.select :holdingCoil
@23:25:29.93 ASV.dial :waste1
@23:25:30.31 ATV.dial :air3
@23:25:31.67 AS.reconfigure ASslow1
@23:25:31.73 AS.seek 0.51ml
@23:25:34.01 delay 2 seconds
@23:25:36.03 ATV.dial :bleach
@23:25:36.65 AS.seek 0.81ml
@23:26:37.23 delay 3 seconds
@23:26:40.26 ATV.dial :air3
@23:26:40.84 delay 3 seconds
@23:26:43.91 AS.seek 0.835ml
@23:26:49.20 delay 3 seconds
@23:26:52.23 ATV.dial :bleach
@23:26:52.87 AS.seek 0.82ml
@23:26:56.28 delay 3 seconds
@23:26:59.31 ARV.dial :waste
@23:26:59.94 delay 3 seconds
@23:27:02.97 ATV.dial :ARV
@23:27:03.90 AS.reconfigure ASconfig
@23:27:03.95 AS.seek 0.47ml
@23:27:24.34 AFV.select :carrierSolution
@23:27:24.46 delay 2 seconds
@23:27:26.48 AS.seek 1ml
@23:27:57.27 delay 1 second
@23:27:58.29 AFV.select :holdingCoil
@23:27:58.40 delay 1 second
@23:27:59.42 AS.reconfigure ASfast
@23:27:59.48 AS.seek 0ml
@23:28:26.84 AFV.select :carrierSolution
@23:28:26.96 delay 2 seconds
@23:28:28.98 AS.reconfigure ASconfig
@23:28:29.03 AS.seek 1ml
@23:29:26.80 delay 1 second
@23:29:27.82 AFV.select :holdingCoil
@23:29:27.93 delay 1 second
@23:29:28.95 AS.reconfigure ASfast
@23:29:29.01 AS.seek 0ml
@23:29:56.34 AFV.select :carrierSolution
@23:29:56.46 delay 2 seconds
@23:29:58.48 AS.reconfigure ASconfig
@23:29:58.53 AS.seek 1ml
@23:30:56.31 delay 1 second
@23:30:57.33 AFV.select :holdingCoil
@23:30:57.44 delay 1 second
@23:30:58.46 AS.reconfigure ASfast
@23:30:58.52 AS.seek 0ml
@23:31:25.86 ARV.dial between carrier and air1
@23:31:26.39 ATV.dial :air3
@23:31:27.76 AS.reconfigure ASslow1
@23:31:27.82 AS.seek 0.01ml
@23:31:30.19 delay 2 seconds
@23:31:32.22 ATV.dial :bleach
@23:31:32.86 AS.seek 0.81ml
@23:34:13.97 delay 3 seconds
@23:34:17.00 ATV.dial :air3
@23:34:17.58 delay 3 seconds
@23:34:20.65 AS.seek 0.835ml
@23:34:26.01 delay 3 seconds
@23:34:29.04 ATV.dial :bleach
@23:34:29.69 AS.seek 0.82ml
@23:34:32.97 delay 3 seconds
@23:34:36.00 ATV.dial PCR
@23:34:36.43 AS.reconfigure ASconfig
@23:34:36.48 AS.seek 0ml
@23:35:23.89 PCR.program :bleachProfile
@23:35:23.96 PCR program should complete in < 2:45
@23:35:23.97 PCR.run 10:00
@23:38:23.22 PCR.off
@23:38:23.27 AFV.select :carrierSolution
@23:38:23.38 delay 2 seconds
@23:38:25.40 AS.seek 1ml
@23:39:23.19 delay 1 second
@23:39:24.21 AFV.select :holdingCoil
@23:39:24.32 delay 1 second
@23:39:25.34 AS.reconfigure ASfast
@23:39:25.40 AS.seek 0ml
@23:39:52.74 delay 3 seconds
@23:39:55.76 AFV.select :carrierSolution
@23:39:55.87 delay 2 seconds
@23:39:57.89 AS.reconfigure ASconfig
@23:39:57.96 AS.seek 1ml
@23:40:55.73 delay 1 second
@23:40:56.75 AFV.select :holdingCoil
@23:40:56.86 delay 1 second
@23:40:57.88 AS.reconfigure ASfast
@23:40:57.94 AS.seek 0ml
@23:41:25.27 delay 3 seconds
@23:41:28.29 AFV.select :carrierSolution
@23:41:28.40 delay 2 seconds
@23:41:30.42 AS.reconfigure ASconfig
@23:41:30.48 AS.seek 1ml
@23:42:28.23 delay 1 second
@23:42:29.25 AFV.select :holdingCoil
@23:42:29.36 delay 1 second
@23:42:30.38 AS.reconfigure ASfast
@23:42:30.44 AS.seek 0ml
@23:42:57.77 delay 3 seconds
@23:43:00.79 AFV.select :carrierSolution
@23:43:00.90 delay 2 seconds
@23:43:02.92 AS.reconfigure ASconfig
@23:43:02.99 AS.seek 1ml
@23:44:00.77 delay 1 second
@23:44:01.79 AFV.select :holdingCoil
@23:44:01.90 delay 1 second
@23:44:02.92 AS.reconfigure ASfast
@23:44:02.98 AS.seek 0ml
@23:44:30.31 delay 3 seconds
@23:44:33.33 AFV.select :carrierSolution
@23:44:33.44 delay 2 seconds
@23:44:35.46 AS.reconfigure ASconfig
@23:44:35.52 AS.seek 1ml
@23:45:33.31 delay 1 second
@23:45:34.33 AFV.select :holdingCoil
@23:45:34.44 delay 1 second
@23:45:35.46 AS.reconfigure ASnorm
@23:45:35.51 AS.seek 0ml
@23:46:26.23 Setting up PCR for AALPP3 -- writing /var/log/esp/AALPP321mar2123h.pcr
@23:46:26.24 Building PCR Slug from ASV with MM2 mix, PP3 primer
@23:46:26.27 Priming with carrier
@23:46:26.28 ATV.dial ARV
@23:46:27.03 ARV.dial :carrier
@23:46:27.39 AS.seek 0.5ml
@23:46:52.97 AFV.select :carrierSolution
@23:46:53.09 delay 2 seconds
@23:46:55.11 AS.reconfigure ASconfig
@23:46:55.17 AS.seek 1ml
@23:47:24.19 delay 1 second
@23:47:25.21 AFV.select :holdingCoil
@23:47:25.32 delay 1 second
@23:47:26.34 ATV.dial :waste
@23:47:28.14 AS.reconfigure ASnorm
@23:47:28.20 AS.seek 0ml
@23:48:18.89 AFV.select :carrierSolution
@23:48:19.02 delay 2 seconds
@23:48:21.04 AS.reconfigure ASconfig
@23:48:21.10 AS.seek 0.5ml
@23:48:50.14 delay 1 second
@23:48:51.16 AFV.select :holdingCoil
@23:48:51.27 Building Slug
@23:48:51.28 ATV.dial :ARV
@23:48:53.05 delay 2 seconds
@23:48:55.08 ARV.dial :air1
@23:48:55.48 delay 2 seconds
@23:48:57.55 AS.reconfigure ASslow2
@23:48:57.61 AS.seek 0.506ml
@23:49:04.20 delay 2 seconds
@23:49:06.23 ARV.dial :MM2
@23:49:07.78 delay 2 seconds
@23:49:09.81 Adding MM2
@23:49:09.88 AS.seek 0.524ml
@23:49:28.39 delay 25 seconds
@23:49:53.42 ARV.dial :PP3
@23:49:53.81 Adding PP3
@23:49:53.83 delay 2 seconds
@23:49:55.90 AS.seek 0.53ml
@23:50:02.36 delay 10 seconds
@23:50:12.40 ARV.dial :air2
@23:50:12.80 adding ARV air
@23:50:12.82 delay 2 seconds
@23:50:14.89 AS.seek 0.536ml
@23:50:21.25 delay 5 seconds
@23:50:26.28 ARV.dial :carrier
@23:50:27.59 delay 7 seconds
@23:50:34.66 AS.seek 0.594ml
@23:51:33.41 delay 5 seconds
@23:51:38.44 ATV.dial :ASV
@23:51:38.86 delay 2 seconds
@23:51:40.93 AS.seek 0.6ml
@23:51:47.31 delay 4 seconds
@23:51:51.34 ATV.dial ARV
@23:51:51.92 delay 2 seconds
@23:51:53.95 ARV.dial :carrier
@23:51:53.96 delay 2 seconds
@23:51:56.46 AS.seek 0.65ml
@23:52:47.17 delay 4 seconds
@23:52:51.20 ATV.dial PCR
@23:52:51.85 ASV.dial :waste1
@23:52:51.87 ARV.dial :air1
@23:52:52.28 Positioning Slug by 0.324ml
@23:52:52.37 AS.seek 0.326ml
@23:58:18.19 delay 5 seconds
@23:58:23.22 ATV.dial ARV
@23:58:24.03 ASV.dial :PCRlock
@23:58:24.44 delay 5 seconds
@23:58:29.47 Running AALPP3 Profile58CIQ2 thermocycling
@23:58:29.48 <PCR> PCR.program Profile58CIQ2
@23:58:29.61 Profile58CIQ2 should complete in < 1:36:15
@23:58:29.62 PCR.run 2:05:18.75
@01:18:26.25CET22-Mar-21 PCR.off
@01:18:26.81 <MFBPCR> Running AALPP3 MeltProfile58 thermocycling
@01:18:26.82 <PCR> PCR.program MeltProfile58
@01:18:26.93 MeltProfile58 should complete in < 12:33
@01:18:26.94 PCR.run 20:41.25
@01:25:53.60 PCR.off
@01:25:54.57 <MFBPCR> PCR for AALPP3 completed -- closed /var/log/esp/AALPP321mar2123h.pcr
@01:25:54.64 Cleaning PCR
@01:25:54.65 AFV.select :carrierSolution
@01:25:54.78 delay 2 seconds
@01:25:56.80 AS.reconfigure ASconfig
@01:25:56.85 AS.seek 0.5ml
@01:26:07.16 delay 1 second
@01:26:08.18 AFV.select :holdingCoil
@01:26:08.29 ASV.dial :waste1
@01:26:08.68 ATV.dial :air3
@01:26:10.05 AS.reconfigure ASslow1
@01:26:10.11 AS.seek 0.51ml
@01:26:12.39 delay 2 seconds
@01:26:14.42 ATV.dial :bleach
@01:26:15.08 AS.seek 0.81ml
@01:27:15.66 delay 3 seconds
@01:27:18.69 ATV.dial :air3
@01:27:19.27 delay 3 seconds
@01:27:22.34 AS.seek 0.835ml
@01:27:27.66 delay 3 seconds
@01:27:30.69 ATV.dial :bleach
@01:27:31.33 AS.seek 0.82ml
@01:27:34.76 delay 3 seconds
@01:27:37.79 ARV.dial :waste
@01:27:38.41 delay 3 seconds
@01:27:41.44 ATV.dial :ARV
@01:27:42.38 AS.reconfigure ASconfig
@01:27:42.43 AS.seek 0.47ml
@01:28:02.83 AFV.select :carrierSolution
@01:28:02.95 delay 2 seconds
@01:28:04.97 AS.seek 1ml
@01:28:35.71 delay 1 second
@01:28:36.73 AFV.select :holdingCoil
@01:28:36.84 delay 1 second
@01:28:37.86 AS.reconfigure ASfast
@01:28:37.92 AS.seek 0ml
@01:29:05.26 AFV.select :carrierSolution
@01:29:05.38 delay 2 seconds
@01:29:07.40 AS.reconfigure ASconfig
@01:29:07.45 AS.seek 1ml
@01:30:05.21 delay 1 second
@01:30:06.23 AFV.select :holdingCoil
@01:30:06.34 delay 1 second
@01:30:07.36 AS.reconfigure ASfast
@01:30:07.41 AS.seek 0ml
@01:30:34.74 AFV.select :carrierSolution
@01:30:34.86 delay 2 seconds
@01:30:36.88 AS.reconfigure ASconfig
@01:30:36.94 AS.seek 1ml
@01:31:34.68 delay 1 second
@01:31:35.70 AFV.select :holdingCoil
@01:31:35.81 delay 1 second
@01:31:36.83 AS.reconfigure ASfast
@01:31:36.89 AS.seek 0ml
@01:32:04.22 ARV.dial between carrier and air1
@01:32:04.74 ATV.dial :air3
@01:32:06.11 AS.reconfigure ASslow1
@01:32:06.17 AS.seek 0.01ml
@01:32:08.57 delay 2 seconds
@01:32:10.60 ATV.dial :bleach
@01:32:11.24 AS.seek 0.81ml
@01:34:52.51 delay 3 seconds
@01:34:55.54 ATV.dial :air3
@01:34:56.12 delay 3 seconds
@01:34:59.19 AS.seek 0.835ml
@01:35:04.59 delay 3 seconds
@01:35:07.62 ATV.dial :bleach
@01:35:08.25 AS.seek 0.82ml
@01:35:11.46 delay 3 seconds
@01:35:14.49 ATV.dial PCR
@01:35:14.92 AS.reconfigure ASconfig
@01:35:14.97 AS.seek 0ml
@01:36:02.37 PCR.program :bleachProfile
@01:36:02.44 PCR program should complete in < 2:45
@01:36:02.45 PCR.run 10:00
@01:39:01.70 PCR.off
@01:39:01.74 AFV.select :carrierSolution
@01:39:01.85 delay 2 seconds
@01:39:03.87 AS.seek 1ml
@01:40:01.66 delay 1 second
@01:40:02.68 AFV.select :holdingCoil
@01:40:02.79 delay 1 second
@01:40:03.81 AS.reconfigure ASfast
@01:40:03.87 AS.seek 0ml
@01:40:31.19 delay 3 seconds
@01:40:34.21 AFV.select :carrierSolution
@01:40:34.32 delay 2 seconds
@01:40:36.34 AS.reconfigure ASconfig
@01:40:36.40 AS.seek 1ml
@01:41:34.15 delay 1 second
@01:41:35.17 AFV.select :holdingCoil
@01:41:35.28 delay 1 second
@01:41:36.30 AS.reconfigure ASfast
@01:41:36.36 AS.seek 0ml
@01:42:03.69 delay 3 seconds
@01:42:06.71 AFV.select :carrierSolution
@01:42:06.82 delay 2 seconds
@01:42:08.84 AS.reconfigure ASconfig
@01:42:08.91 AS.seek 1ml
@01:43:06.66 delay 1 second
@01:43:07.68 AFV.select :holdingCoil
@01:43:07.79 delay 1 second
@01:43:08.81 AS.reconfigure ASfast
@01:43:08.87 AS.seek 0ml
@01:43:36.21 delay 3 seconds
@01:43:39.23 AFV.select :carrierSolution
@01:43:39.34 delay 2 seconds
@01:43:41.36 AS.reconfigure ASconfig
@01:43:41.42 AS.seek 1ml
@01:44:39.16 delay 1 second
@01:44:40.18 AFV.select :holdingCoil
@01:44:40.29 delay 1 second
@01:44:41.31 AS.reconfigure ASfast
@01:44:41.37 AS.seek 0ml
@01:45:08.71 delay 3 seconds
@01:45:11.73 AFV.select :carrierSolution
@01:45:11.84 delay 2 seconds
@01:45:13.86 AS.reconfigure ASconfig
@01:45:13.93 AS.seek 1ml
@01:46:11.71 delay 1 second
@01:46:12.73 AFV.select :holdingCoil
@01:46:12.84 delay 1 second
@01:46:13.86 AS.reconfigure ASnorm
@01:46:13.91 AS.seek 0ml
@01:47:04.63 Setting up PCR for ACOPP5 -- writing /var/log/esp/ACOPP521mar2201h.pcr
@01:47:04.64 Building PCR Slug from ASV with MM3 mix, PP5 primer
@01:47:04.67 Priming with carrier
@01:47:04.69 ATV.dial ARV
@01:47:05.33 ARV.dial :carrier
@01:47:05.69 AS.seek 0.5ml
@01:47:31.21 AFV.select :carrierSolution
@01:47:31.33 delay 2 seconds
@01:47:33.35 AS.reconfigure ASconfig
@01:47:33.41 AS.seek 1ml
@01:48:02.41 delay 1 second
@01:48:03.43 AFV.select :holdingCoil
@01:48:03.54 delay 1 second
@01:48:04.56 ATV.dial :waste
@01:48:06.36 AS.reconfigure ASnorm
@01:48:06.42 AS.seek 0ml
@01:48:57.13 AFV.select :carrierSolution
@01:48:57.26 delay 2 seconds
@01:48:59.28 AS.reconfigure ASconfig
@01:48:59.34 AS.seek 0.5ml
@01:49:28.37 delay 1 second
@01:49:29.39 AFV.select :holdingCoil
@01:49:29.50 Building Slug
@01:49:29.51 ATV.dial :ARV
@01:49:31.27 delay 2 seconds
@01:49:33.30 ARV.dial :air1
@01:49:33.70 delay 2 seconds
@01:49:35.77 AS.reconfigure ASslow2
@01:49:35.83 AS.seek 0.506ml
@01:49:42.24 delay 2 seconds
@01:49:44.27 ARV.dial :MM3
@01:49:45.35 delay 2 seconds
@01:49:47.38 Adding MM3
@01:49:47.46 AS.seek 0.524ml
@01:50:06.02 delay 25 seconds
@01:50:31.05 ARV.dial :PP5
@01:50:31.46 Adding PP5
@01:50:31.48 delay 2 seconds
@01:50:33.55 AS.seek 0.53ml
@01:50:39.80 delay 10 seconds
@01:50:49.84 ARV.dial :air3
@01:50:50.64 adding ARV air
@01:50:50.66 delay 2 seconds
@01:50:52.73 AS.seek 0.536ml
@01:50:59.01 delay 5 seconds
@01:51:04.04 ARV.dial :carrier
@01:51:05.37 delay 7 seconds
@01:51:12.45 AS.seek 0.594ml
@01:52:10.90 delay 5 seconds
@01:52:15.93 ATV.dial :ASV
@01:52:16.35 delay 2 seconds
@01:52:18.42 AS.seek 0.6ml
@01:52:24.69 delay 4 seconds
@01:52:28.72 ATV.dial ARV
@01:52:29.30 delay 2 seconds
@01:52:31.33 ARV.dial :carrier
@01:52:31.34 delay 2 seconds
@01:52:33.41 AS.seek 0.65ml
@01:53:23.93 delay 4 seconds
@01:53:27.96 ATV.dial PCR
@01:53:28.60 ASV.dial :waste1
@01:53:28.62 ARV.dial :air1
@01:53:29.03 Positioning Slug by 0.324ml
@01:53:29.12 AS.seek 0.326ml
@01:58:55.21 delay 5 seconds
@01:59:00.24 ATV.dial ARV
@01:59:01.05 ASV.dial :PCRlock
@01:59:01.46 delay 5 seconds
@01:59:06.49 Running ACOPP5 ProfileIPc thermocycling
@01:59:06.50 <PCR> PCR.program ProfileIPc
@01:59:06.62 ProfileIPc should complete in < 1:26:35
@01:59:06.63 PCR.run 1:53:13.75
@03:06:49.74 PCR.off
@03:06:50.29 <MFBPCR> PCR for ACOPP5 completed -- closed /var/log/esp/ACOPP521mar2201h.pcr
@03:06:50.36 Cleaning PCR
@03:06:50.37 AFV.select :carrierSolution
@03:06:50.50 delay 2 seconds
@03:06:52.52 AS.reconfigure ASconfig
@03:06:52.57 AS.seek 0.5ml
@03:07:02.92 delay 1 second
@03:07:03.94 AFV.select :holdingCoil
@03:07:04.05 ASV.dial :waste1
@03:07:04.43 ATV.dial :air3
@03:07:05.80 AS.reconfigure ASslow1
@03:07:05.86 AS.seek 0.51ml
@03:07:08.14 delay 2 seconds
@03:07:10.17 ATV.dial :bleach
@03:07:10.82 AS.seek 0.81ml
@03:08:11.45 delay 3 seconds
@03:08:14.48 ATV.dial :air3
@03:08:15.06 delay 3 seconds
@03:08:18.13 AS.seek 0.835ml
@03:08:23.44 delay 3 seconds
@03:08:26.47 ATV.dial :bleach
@03:08:27.12 AS.seek 0.82ml
@03:08:30.45 delay 3 seconds
@03:08:33.48 ARV.dial :waste
@03:08:34.10 delay 3 seconds
@03:08:37.13 ATV.dial :ARV
@03:08:38.07 AS.reconfigure ASconfig
@03:08:38.12 AS.seek 0.47ml
@03:08:58.53 AFV.select :carrierSolution
@03:08:58.65 delay 2 seconds
@03:09:00.67 AS.seek 1ml
@03:09:31.45 delay 1 second
@03:09:32.47 AFV.select :holdingCoil
@03:09:32.58 delay 1 second
@03:09:33.60 AS.reconfigure ASfast
@03:09:33.66 AS.seek 0ml
@03:10:01.03 AFV.select :carrierSolution
@03:10:01.15 delay 2 seconds
@03:10:03.17 AS.reconfigure ASconfig
@03:10:03.22 AS.seek 1ml
@03:11:00.98 delay 1 second
@03:11:02.00 AFV.select :holdingCoil
@03:11:02.11 delay 1 second
@03:11:03.13 AS.reconfigure ASfast
@03:11:03.18 AS.seek 0ml
@03:11:30.50 AFV.select :carrierSolution
@03:11:30.62 delay 2 seconds
@03:11:32.64 AS.reconfigure ASconfig
@03:11:32.69 AS.seek 1ml
@03:12:30.45 delay 1 second
@03:12:31.47 AFV.select :holdingCoil
@03:12:31.58 delay 1 second
@03:12:32.60 AS.reconfigure ASfast
@03:12:32.65 AS.seek 0ml
@03:13:00.00 ARV.dial between carrier and air1
@03:13:00.51 ATV.dial :air3
@03:13:01.88 AS.reconfigure ASslow1
@03:13:01.93 AS.seek 0.01ml
@03:13:04.34 delay 2 seconds
@03:13:06.37 ATV.dial :bleach
@03:13:07.02 AS.seek 0.81ml
@03:15:48.18 delay 3 seconds
@03:15:51.21 ATV.dial :air3
@03:15:51.79 delay 3 seconds
@03:15:54.86 AS.seek 0.835ml
@03:16:00.14 delay 3 seconds
@03:16:03.17 ATV.dial :bleach
@03:16:03.81 AS.seek 0.82ml
@03:16:07.12 delay 3 seconds
@03:16:10.15 ATV.dial PCR
@03:16:10.58 AS.reconfigure ASconfig
@03:16:10.63 AS.seek 0ml
@03:16:58.04 PCR.program :bleachProfile
@03:16:58.11 PCR program should complete in < 2:45
@03:16:58.12 PCR.run 10:00
@03:19:57.37 PCR.off
@03:19:57.41 AFV.select :carrierSolution
@03:19:57.52 delay 2 seconds
@03:19:59.54 AS.seek 1ml
@03:20:57.29 delay 1 second
@03:20:58.31 AFV.select :holdingCoil
@03:20:58.42 delay 1 second
@03:20:59.44 AS.reconfigure ASfast
@03:20:59.50 AS.seek 0ml
@03:21:26.84 delay 3 seconds
@03:21:29.86 AFV.select :carrierSolution
@03:21:29.97 delay 2 seconds
@03:21:31.99 AS.reconfigure ASconfig
@03:21:32.05 AS.seek 1ml
@03:22:29.81 delay 1 second
@03:22:30.83 AFV.select :holdingCoil
@03:22:30.94 delay 1 second
@03:22:31.96 AS.reconfigure ASfast
@03:22:32.02 AS.seek 0ml
@03:22:59.37 delay 3 seconds
@03:23:02.39 AFV.select :carrierSolution
@03:23:02.50 delay 2 seconds
@03:23:04.52 AS.reconfigure ASconfig
@03:23:04.59 AS.seek 1ml
@03:24:02.34 delay 1 second
@03:24:03.36 AFV.select :holdingCoil
@03:24:03.47 delay 1 second
@03:24:04.49 AS.reconfigure ASfast
@03:24:04.55 AS.seek 0ml
@03:24:31.89 delay 3 seconds
@03:24:34.91 AFV.select :carrierSolution
@03:24:35.02 delay 2 seconds
@03:24:37.04 AS.reconfigure ASconfig
@03:24:37.10 AS.seek 1ml
@03:25:34.84 delay 1 second
@03:25:35.86 AFV.select :holdingCoil
@03:25:35.97 delay 1 second
@03:25:36.99 AS.reconfigure ASfast
@03:25:37.05 AS.seek 0ml
@03:26:04.39 delay 3 seconds
@03:26:07.41 AFV.select :carrierSolution
@03:26:07.52 delay 2 seconds
@03:26:09.54 AS.reconfigure ASconfig
@03:26:09.60 AS.seek 1ml
@03:27:07.37 delay 1 second
@03:27:08.39 AFV.select :holdingCoil
@03:27:08.50 delay 1 second
@03:27:09.52 AS.reconfigure ASnorm
@03:27:09.58 AS.seek 0ml
@03:28:00.29 Setting up PCR for AIPc -- writing /var/log/esp/AIPc21mar2203h.pcr
@03:28:00.30 Building PCR Slug from ASV with MM3 mix, PP6 primer
@03:28:00.33 Priming with carrier
@03:28:00.35 ATV.dial ARV
@03:28:01.00 ARV.dial :carrier
@03:28:01.35 AS.seek 0.5ml
@03:28:26.95 AFV.select :carrierSolution
@03:28:27.07 delay 2 seconds
@03:28:29.09 AS.reconfigure ASconfig
@03:28:29.15 AS.seek 1ml
@03:28:58.17 delay 1 second
@03:28:59.19 AFV.select :holdingCoil
@03:28:59.30 delay 1 second
@03:29:00.32 ATV.dial :waste
@03:29:02.10 AS.reconfigure ASnorm
@03:29:02.16 AS.seek 0ml
@03:29:52.86 AFV.select :carrierSolution
@03:29:52.98 delay 2 seconds
@03:29:55.00 AS.reconfigure ASconfig
@03:29:55.06 AS.seek 0.5ml
@03:30:24.09 delay 1 second
@03:30:25.11 AFV.select :holdingCoil
@03:30:25.22 Building Slug
@03:30:25.23 ATV.dial :ARV
@03:30:26.98 delay 2 seconds
@03:30:29.01 ARV.dial :air1
@03:30:29.41 delay 2 seconds
@03:30:31.48 AS.reconfigure ASslow2
@03:30:31.54 AS.seek 0.506ml
@03:30:37.89 delay 2 seconds
@03:30:39.92 ARV.dial :MM3
@03:30:41.00 delay 2 seconds
@03:30:43.03 Adding MM3
@03:30:43.10 AS.seek 0.524ml
@03:31:01.79 delay 25 seconds
@03:31:26.82 ARV.dial :PP6
@03:31:27.22 Adding PP6
@03:31:27.23 delay 2 seconds
@03:31:29.30 AS.seek 0.53ml
@03:31:35.68 delay 10 seconds
@03:31:45.72 ARV.dial :air1
@03:31:46.58 adding ARV air
@03:31:46.59 delay 2 seconds
@03:31:48.66 AS.seek 0.536ml
@03:31:55.06 delay 5 seconds
@03:32:00.09 ARV.dial :carrier
@03:32:00.50 delay 7 seconds
@03:32:07.99 AS.seek 0.594ml
@03:33:06.64 delay 5 seconds
@03:33:11.67 ATV.dial :ASV
@03:33:12.09 delay 2 seconds
@03:33:14.17 AS.seek 0.6ml
@03:33:20.34 delay 4 seconds
@03:33:24.37 ATV.dial ARV
@03:33:24.93 delay 2 seconds
@03:33:26.96 ARV.dial :carrier
@03:33:26.97 delay 2 seconds
@03:33:29.05 AS.seek 0.65ml
@03:34:19.54 delay 4 seconds
@03:34:23.57 ATV.dial PCR
@03:34:24.22 ASV.dial :waste1
@03:34:24.24 ARV.dial :air1
@03:34:24.66 Positioning Slug by 0.324ml
@03:34:24.74 AS.seek 0.326ml
@03:39:50.73 delay 5 seconds
@03:39:55.76 ATV.dial ARV
@03:39:56.57 ASV.dial :PCRlock
@03:39:56.98 delay 5 seconds
@03:40:02.01 Running AIPc ProfileIPc thermocycling
@03:40:02.02 <PCR> PCR.program ProfileIPc
@03:40:02.14 ProfileIPc should complete in < 1:26:35
@03:40:02.15 PCR.run 1:53:13.75
@04:47:25.69 PCR.off
@04:47:26.62 <MFBPCR> PCR for AIPc completed -- closed /var/log/esp/AIPc21mar2203h.pcr
@04:47:26.64 Full MFB Decontamination
@04:47:26.65 Decon extraction column and decon extSrc
@04:47:26.69 SPE.hold 2:46:40,55C
@04:47:26.74 ASV.dial :SPEdelivery
@04:47:27.42 delay 20 seconds
@04:47:47.45 AFV.select :carrierSolution
@04:47:47.56 delay 2 seconds
@04:47:49.58 AS.reconfigure ASconfig
@04:47:49.64 AS.seek 0.5ml
@04:47:59.92 delay 1 second
@04:48:00.94 AFV.select :holdingCoil
@04:48:01.05 ATV.dial :air1
@04:48:03.31 AS.reconfigure ASslow1
@04:48:03.37 AS.seek 0.52ml
@04:48:07.64 ATV.dial :ASV
@04:48:09.42 ASV.dial :SPErecovery
@04:48:10.12 AS.seek 0.62ml
@04:48:30.59 ATV.dial :waste
@04:48:32.81 AS.reconfigure ASnorm
@04:48:32.87 AS.seek 0ml
@04:49:04.41 deconEluateRecoveryLine extSrc
@04:49:04.42 ATV.dial :waste
@04:49:04.46 AS.seek 0ml
@04:49:04.61 ATV.dial :air3
@04:49:05.39 AS.reconfigure ASslow1
@04:49:05.45 AS.seek 0.01ml
@04:49:07.72 delay 2 seconds
@04:49:09.75 ATV.dial :bleach
@04:49:10.26 AS.seek 0.31ml
@04:50:10.95 delay 3 seconds
@04:50:13.98 ATV.dial :air3
@04:50:14.61 delay 3 seconds
@04:50:17.68 AS.seek 0.335ml
@04:50:23.03 delay 3 seconds
@04:50:26.06 ATV.dial :bleach
@04:50:26.73 AS.seek 0.32ml
@04:50:29.97 delay 3 seconds
@04:50:33.00 ATV.dial :extSrc
@04:50:33.68 AS.seek 0ml
@04:51:38.41 AFV.select :carrierSolution
@04:51:38.53 delay 2 seconds
@04:51:40.55 AS.reconfigure ASconfig
@04:51:40.61 AS.seek 0.5ml
@04:52:09.63 delay 1 second
@04:52:10.65 AFV.select :holdingCoil
@04:52:10.76 ATV.dial :extSrc
@04:52:10.78 AS.reconfigure ASslow1
@04:52:10.85 AS.seek 0ml
@04:53:51.66 AFV.select :carrierSolution
@04:53:51.78 delay 2 seconds
@04:53:53.80 AS.reconfigure ASconfig
@04:53:53.85 AS.seek 0.5ml
@04:54:22.89 delay 1 second
@04:54:23.91 AFV.select :holdingCoil
@04:54:24.02 ATV.dial :extSrc
@04:54:24.04 AS.reconfigure ASslow1
@04:54:24.11 AS.seek 0ml
@04:56:04.92 AFV.select :carrierSolution
@04:56:05.04 delay 2 seconds
@04:56:07.06 AS.reconfigure ASconfig
@04:56:07.12 AS.seek 0.5ml
@04:56:36.13 delay 1 second
@04:56:37.15 AFV.select :holdingCoil
@04:56:37.26 ATV.dial :extSrc
@04:56:37.28 AS.reconfigure ASslow1
@04:56:37.35 AS.seek 0ml
@04:58:18.17 AFV.select :carrierSolution
@04:58:18.29 delay 2 seconds
@04:58:20.31 AS.reconfigure ASconfig
@04:58:20.37 AS.seek 0.5ml
@04:58:49.39 delay 1 second
@04:58:50.41 AFV.select :holdingCoil
@04:58:50.52 ATV.dial :extSrc
@04:58:50.54 AS.reconfigure ASslow1
@04:58:50.61 AS.seek 0ml
@05:00:31.42 ATV.dial :air2
@05:00:32.83 AS.reconfigure ASslow0
@05:00:32.89 AS.seek 0.5ml
@05:01:23.52 ATV.dial :extSrc
@05:01:25.09 AS.seek 0.02ml
@05:02:13.64 delay 1 second
@05:02:14.67 ATV.dial :waste
@05:02:16.04 AS.reconfigure ASconfig
@05:02:16.09 AS.seek 0ml
@05:02:17.52 AFV.select :holdingCoil
@05:02:17.54 ATV.dial :waste
@05:02:17.56 AS.seek 0ml
@05:02:17.65 AFV.select :carrierSolution
@05:02:17.76 delay 2 seconds
@05:02:19.78 AS.seek 0.5ml
@05:02:48.81 delay 1 second
@05:02:49.83 AFV.select :holdingCoil
@05:02:49.94 ATV.dial :air3
@05:02:50.83 AS.reconfigure ASslow1
@05:02:50.89 AS.seek 0.52ml
@05:02:55.16 ATV.dial :air3
@05:02:55.25 AS.seek 0.53ml
@05:02:57.52 delay 2 seconds
@05:02:59.55 ATV.dial :bleach
@05:03:00.01 AS.seek 0.71ml
@05:03:36.58 delay 3 seconds
@05:03:39.61 ATV.dial :air3
@05:03:40.19 delay 3 seconds
@05:03:43.26 AS.seek 0.735ml
@05:03:48.60 delay 3 seconds
@05:03:51.63 ATV.dial :bleach
@05:03:52.28 AS.seek 0.72ml
@05:03:55.61 delay 3 seconds
@05:03:58.64 ATV.dial ASV
@05:03:59.75 ASV.dial :SPErecovery
@05:04:00.28 AS.seek 0.365ml
@05:05:11.94 ATV.dial :waste
@05:05:13.79 AS.reconfigure ASnorm
@05:05:13.85 AS.seek 0ml
@05:05:32.58 ATV.dial :air3
@05:05:33.31 AS.reconfigure ASslow1
@05:05:33.37 AS.seek 0.01ml
@05:05:35.72 delay 2 seconds
@05:05:37.75 ATV.dial :bleach
@05:05:38.23 AS.seek 0.58ml
@05:07:33.13 delay 3 seconds
@05:07:36.16 ATV.dial :air3
@05:07:36.75 delay 3 seconds
@05:07:39.83 AS.seek 0.605ml
@05:07:45.22 delay 3 seconds
@05:07:48.25 ATV.dial :bleach
@05:07:48.89 AS.seek 0.59ml
@05:07:52.19 delay 3 seconds
@05:07:55.22 ATV.dial :ASV
@05:07:56.31 ASV.dial :wasteR
@05:07:56.71 AS.seek 0ml
@05:09:55.61 delay 1:00
@05:10:55.65 ATV.dial ASV
@05:10:55.66 AFV.select :carrierSolution
@05:10:55.78 delay 2 seconds
@05:10:57.80 AS.reconfigure ASconfig
@05:10:57.86 AS.seek 1ml
@05:11:55.61 delay 1 second
@05:11:56.63 AFV.select :holdingCoil
@05:11:56.74 delay 1 second
@05:11:57.76 ASV.dial :wasteR
@05:11:57.77 AS.reconfigure ASslow1
@05:11:57.84 AS.seek 0ml
@05:15:19.22 delay 10 seconds
@05:15:29.25 ATV.dial :air3
@05:15:30.84 AS.seek 0.01ml
@05:15:33.22 delay 2 seconds
@05:15:35.25 ATV.dial :bleach
@05:15:35.91 AS.seek 0.58ml
@05:17:30.88 delay 3 seconds
@05:17:33.91 ATV.dial :air3
@05:17:34.49 delay 3 seconds
@05:17:37.56 AS.seek 0.605ml
@05:17:42.90 delay 3 seconds
@05:17:45.93 ATV.dial :bleach
@05:17:46.56 AS.seek 0.59ml
@05:17:49.88 delay 3 seconds
@05:17:52.91 ATV.dial ASV
@05:17:54.00 ASV.dial :SPEdelivery
@05:17:54.40 AS.seek 0ml
@05:19:53.30 delay 1:00
@05:20:53.33 AFV.select :carrierSolution
@05:20:53.44 delay 2 seconds
@05:20:55.46 AS.reconfigure ASconfig
@05:20:55.52 AS.seek 1ml
@05:21:53.27 delay 1 second
@05:21:54.29 AFV.select :holdingCoil
@05:21:54.40 delay 1 second
@05:21:55.42 ATV.dial ASV
@05:21:55.43 ASV.dial :SPEdelivery
@05:21:55.45 AS.reconfigure ASslow1
@05:21:55.52 AS.seek 0ml
@05:25:16.91 delay 10 seconds
@05:25:26.94 ATV.dial :air2
@05:25:27.82 AS.reconfigure ASslow0
@05:25:27.87 AS.seek 0.8ml
@05:26:48.69 delay 3 seconds
@05:26:51.72 ATV.dial :ASV
@05:26:52.77 ASV.dial :SPEdelivery
@05:26:52.79 AS.reconfigure ASslow1
@05:26:52.86 AS.seek 0.03ml
@05:29:28.11 delay 20 seconds
@05:29:48.15 ATV.dial :waste,via: :air3
@05:29:49.98 AS.reconfigure ASconfig
@05:29:50.03 AS.seek 0ml
@05:29:52.08 SPE.coast
@05:29:52.16 Removing Bleach from Mixing Coil
@05:29:52.17 AFV.select :carrierSolution
@05:29:52.29 delay 2 seconds
@05:29:54.31 AS.seek 0.5ml
@05:30:23.33 delay 1 second
@05:30:24.35 AFV.select :holdingCoil
@05:30:24.46 ATV.dial :ASV
@05:30:26.42 ASV.dial :SPErecovery
@05:30:27.08 AS.reconfigure ASslow1
@05:30:27.14 AS.seek 0.73ml
@05:31:13.66 ASV.dial :wasteD
@05:31:14.28 AS.reconfigure ASnorm
@05:31:14.33 AS.seek 0ml
@05:31:51.44 AFV.select :carrierSolution
@05:31:51.56 delay 2 seconds
@05:31:53.58 AS.reconfigure ASconfig
@05:31:53.64 AS.seek 0.5ml
@05:32:22.74 delay 1 second
@05:32:23.76 AFV.select :holdingCoil
@05:32:23.87 ATV.dial ASV
@05:32:23.88 ASV.dial :SPErecovery
@05:32:24.55 AS.reconfigure ASslow0
@05:32:24.61 AS.seek 0.3ml
@05:32:45.02 ATV.dial :air2
@05:32:45.94 AS.reconfigure ASslow1
@05:32:46.00 AS.seek 0.32ml
@05:32:50.36 ATV.dial ASV
@05:32:51.47 AS.reconfigure ASslow0
@05:32:51.53 AS.seek 0.57ml
@05:33:16.99 ASV.dial :wasteD
@05:33:17.61 AS.reconfigure ASnorm
@05:33:17.66 AS.seek 0ml
@05:33:47.17 AFV.select :carrierSolution
@05:33:47.29 delay 2 seconds
@05:33:49.31 AS.reconfigure ASconfig
@05:33:49.37 AS.seek 0.5ml
@05:34:18.38 delay 1 second
@05:34:19.40 AFV.select :holdingCoil
@05:34:19.51 ATV.dial ASV
@05:34:19.52 ASV.dial :SPErecovery
@05:34:20.19 AS.reconfigure ASslow0
@05:34:20.25 AS.seek 0.3ml
@05:34:40.64 ATV.dial :air2
@05:34:41.73 AS.reconfigure ASslow1
@05:34:41.79 AS.seek 0.32ml
@05:34:46.11 ATV.dial ASV
@05:34:47.22 AS.reconfigure ASslow0
@05:34:47.28 AS.seek 0.57ml
@05:35:12.75 ASV.dial :wasteD
@05:35:13.36 AS.reconfigure ASnorm
@05:35:13.41 AS.seek 0ml
@05:35:42.49 ATV.dial :air2
@05:35:43.54 AS.reconfigure ASslow0
@05:35:43.60 AS.seek 0.3ml
@05:36:14.11 ATV.dial :ASV
@05:36:15.16 ASV.dial :wasteD
@05:36:15.18 AS.reconfigure ASconfig
@05:36:15.25 AS.seek 0.05ml
@05:36:29.92 ATV.dial :waste
@05:36:31.90 AS.seek 0ml
@05:36:35.08 ASV.dial :SPEdelivery
@05:36:36.19 Cleaning PCR
@05:36:36.20 AFV.select :carrierSolution
@05:36:36.32 delay 2 seconds
@05:36:38.34 AS.seek 0.5ml
@05:37:07.39 delay 1 second
@05:37:08.41 AFV.select :holdingCoil
@05:37:08.52 ASV.dial :waste1
@05:37:08.89 ATV.dial :air3
@05:37:09.61 AS.reconfigure ASslow1
@05:37:09.67 AS.seek 0.51ml
@05:37:12.02 delay 2 seconds
@05:37:14.05 ATV.dial :bleach
@05:37:14.52 AS.seek 0.81ml
@05:38:15.13 delay 3 seconds
@05:38:18.16 ATV.dial :air3
@05:38:18.74 delay 3 seconds
@05:38:21.81 AS.seek 0.835ml
@05:38:27.14 delay 3 seconds
@05:38:30.17 ATV.dial :bleach
@05:38:30.81 AS.seek 0.82ml
@05:38:34.11 delay 3 seconds
@05:38:37.14 ARV.dial :waste
@05:38:37.77 delay 3 seconds
@05:38:40.80 ATV.dial :ARV
@05:38:41.72 AS.reconfigure ASconfig
@05:38:41.77 AS.seek 0.47ml
@05:39:02.19 AFV.select :carrierSolution
@05:39:02.31 delay 2 seconds
@05:39:04.33 AS.seek 1ml
@05:39:35.11 delay 1 second
@05:39:36.13 AFV.select :holdingCoil
@05:39:36.24 delay 1 second
@05:39:37.26 AS.reconfigure ASfast
@05:39:37.31 AS.seek 0ml
@05:40:04.66 AFV.select :carrierSolution
@05:40:04.78 delay 2 seconds
@05:40:06.80 AS.reconfigure ASconfig
@05:40:06.85 AS.seek 1ml
@05:41:04.63 delay 1 second
@05:41:05.65 AFV.select :holdingCoil
@05:41:05.76 delay 1 second
@05:41:06.78 AS.reconfigure ASfast
@05:41:06.84 AS.seek 0ml
@05:41:34.17 AFV.select :carrierSolution
@05:41:34.29 delay 2 seconds
@05:41:36.31 AS.reconfigure ASconfig
@05:41:36.36 AS.seek 1ml
@05:42:34.14 delay 1 second
@05:42:35.16 AFV.select :holdingCoil
@05:42:35.27 delay 1 second
@05:42:36.29 AS.reconfigure ASfast
@05:42:36.35 AS.seek 0ml
@05:43:03.69 ARV.dial between carrier and air1
@05:43:04.21 ATV.dial :air3
@05:43:05.56 AS.reconfigure ASslow1
@05:43:05.62 AS.seek 0.01ml
@05:43:07.92 delay 2 seconds
@05:43:09.95 ATV.dial :bleach
@05:43:10.59 AS.seek 0.81ml
@05:45:51.82 delay 3 seconds
@05:45:54.85 ATV.dial :air3
@05:45:55.42 delay 3 seconds
@05:45:58.49 AS.seek 0.835ml
@05:46:03.79 delay 3 seconds
@05:46:06.82 ATV.dial :bleach
@05:46:07.45 AS.seek 0.82ml
@05:46:10.75 delay 3 seconds
@05:46:13.78 ATV.dial PCR
@05:46:14.22 AS.reconfigure ASconfig
@05:46:14.27 AS.seek 0ml
@05:47:01.69 PCR.program :bleachProfile
@05:47:01.76 PCR program should complete in < 2:45
@05:47:01.77 PCR.run 10:00
@05:50:04.06 PCR.off
@05:50:04.11 AFV.select :carrierSolution
@05:50:04.22 delay 2 seconds
@05:50:06.24 AS.seek 1ml
@05:51:04.00 delay 1 second
@05:51:05.02 AFV.select :holdingCoil
@05:51:05.13 delay 1 second
@05:51:06.15 AS.reconfigure ASfast
@05:51:06.21 AS.seek 0ml
@05:51:33.57 delay 3 seconds
@05:51:36.59 AFV.select :carrierSolution
@05:51:36.70 delay 2 seconds
@05:51:38.72 AS.reconfigure ASconfig
@05:51:38.78 AS.seek 1ml
@05:52:36.53 delay 1 second
@05:52:37.55 AFV.select :holdingCoil
@05:52:37.66 delay 1 second
@05:52:38.68 AS.reconfigure ASfast
@05:52:38.73 AS.seek 0ml
@05:53:06.08 delay 3 seconds
@05:53:09.10 AFV.select :carrierSolution
@05:53:09.21 delay 2 seconds
@05:53:11.23 AS.reconfigure ASconfig
@05:53:11.29 AS.seek 1ml
@05:54:09.05 delay 1 second
@05:54:10.07 AFV.select :holdingCoil
@05:54:10.18 delay 1 second
@05:54:11.20 AS.reconfigure ASfast
@05:54:11.25 AS.seek 0ml
@05:54:38.58 delay 3 seconds
@05:54:41.60 AFV.select :carrierSolution
@05:54:41.71 delay 2 seconds
@05:54:43.73 AS.reconfigure ASconfig
@05:54:43.79 AS.seek 1ml
@05:55:41.53 delay 1 second
@05:55:42.55 AFV.select :holdingCoil
@05:55:42.66 delay 1 second
@05:55:43.68 AS.reconfigure ASfast
@05:55:43.74 AS.seek 0ml
@05:56:11.07 delay 3 seconds
@05:56:14.09 AFV.select :carrierSolution
@05:56:14.20 delay 2 seconds
@05:56:16.22 AS.reconfigure ASconfig
@05:56:16.28 AS.seek 1ml
@05:57:14.05 delay 1 second
@05:57:15.07 AFV.select :holdingCoil
@05:57:15.18 delay 1 second
@05:57:16.20 AS.reconfigure ASnorm
@05:57:16.26 AS.seek 0ml
@05:58:06.98 AFV.select :carrierSolution
@05:58:07.09 delay 2 seconds
@05:58:09.11 AS.reconfigure ASconfig
@05:58:09.16 AS.seek 1ml
@05:59:06.91 delay 1 second
@05:59:07.93 AFV.select :holdingCoil
@05:59:08.04 delay 1 second
@05:59:09.06 ATV.dial :waste
@05:59:10.36 AS.reconfigure ASnorm
@05:59:10.41 AS.seek 0.5ml
@05:59:35.96 ATV.dial :waste2
@05:59:37.34 AS.seek 0ml
@06:00:02.91 <mwj> Session Ended
@13:35:11.22CET22-Mar-21 <MAIN> ESP2 v3.30 I2C Gateway v3.06 for core bus
@13:35:13.31 
Configuration for mer 7/20/20 brent@mbari.org
/home/esp/esp2/type/shallow/mer/configure.rb
@13:35:13.40 
Configuration for MFB3 by preston@mbari.org
/home/esp/esp2/type/mfb/MFB3/configure.rb
@13:35:17.74 Polling Can every 10:00
@13:35:19.45 CS.configure :default
@13:35:19.49 CS.hold
@13:35:19.55 CS.configure :default
@13:35:19.63 PS.configure :default
@13:35:19.71 SS.configure :default
@13:35:19.75 SS.hold
@13:35:19.79 SS.configure :default
@13:35:19.87 CC.configure :default
@13:35:19.91 CC.hold
@13:35:19.95 CC.configure :default
@13:35:20.03 PC.configure :default
@13:35:20.07 PC.hold
@13:35:20.12 PC.configure :default
@13:35:20.22 Forearm.configure :loose
@13:35:20.26 Forearm.hold
@13:35:20.31 Forearm.configure :loose
@13:35:20.39 Elbow.configure :loose
@13:35:20.42 Elbow.hold
@13:35:20.47 Elbow.configure :loose
@13:35:20.56 SC.configure :loose
@13:35:20.60 SC.hold
@13:35:20.65 SC.configure :loose
@13:35:20.72 SE.configure :default
@13:35:20.76 SE.hold
@13:35:20.80 SE.configure :default
@13:35:20.84 CH.coast
@13:35:20.89 CH.configure :default
@13:35:20.91 PH.coast
@13:35:20.95 PH.configure :default
@13:35:21.05 SPE.coast
@13:35:21.10 SPE.configure SPEconfig
@13:35:21.17 AS.configure ASconfig
@13:35:21.21 AS.hold
@13:35:21.26 AS.configure ASconfig
@13:35:21.31 PCR.off
@13:35:21.34 PCR.pidtemp= 50,0.1,0,300,25,25
@13:35:21.37 PCR.caltemp= 2.5051,0.0372,0
@13:35:21.40 All dwarf controllers running firmware version 3.75
@13:35:21.57 Cleanly exited at 13:34:09CET22-Mar-21
@13:35:21.60 Loading /home/esp/esp2/mission/GENOMAP.rb mission
@13:35:21.74 <MFBPCR> Extracting Nucleic Acid from extSrc
@13:35:21.77 SPE.hold 2:46:40,55C
@13:35:21.81 ASV.dial :SPEdelivery
@13:35:22.24 delay 20 seconds
@13:35:33.58 <mwj> Session Begun
@13:35:42.27 <MFBPCR> AFV.select :holdingCoil
@13:35:42.40 ATV.dial :air2
@13:35:43.65 AS.reconfigure ASslow1
@13:35:43.71 AS.seek 0.07ml
@13:35:58.23 delay 3 seconds
@13:36:01.26 ATV.dial :extSrc
@13:36:02.74 AS.seek 0.47ml
@13:37:23.54 delay 5 seconds
@13:37:28.62 Solid Phase Extraction Heater at 38.7C
@13:37:28.63 ATV.dial ASV
@13:37:29.49 ASV.dial :SPEdelivery
@13:37:29.58 AS.seek 0.042ml
@13:38:55.90 delay 10 seconds
@13:39:05.94 Rinsing external lysate line extSrc
@13:39:05.95 AFV.select :carrierSolution
@13:39:06.07 delay 2 seconds
@13:39:08.09 AS.reconfigure ASconfig
@13:39:08.15 AS.seek 1ml
@13:40:03.52 delay 1 second
@13:40:04.54 AFV.select :holdingCoil
@13:40:04.65 delay 1 second
@13:40:05.67 ATV.dial :extSrc
@13:40:06.49 AS.reconfigure ASnorm
@13:40:06.54 AS.seek 0ml
@13:40:57.23 ATV.dial :air2
@13:40:58.83 AS.reconfigure ASslow0
@13:40:58.89 AS.seek 0.05ml
@13:41:04.27 ATV.dial :extSrc
@13:41:05.85 AS.seek 0.01ml
@13:41:10.16 ATV.dial :waste
@13:41:11.53 AS.reconfigure ASconfig
@13:41:11.58 AS.seek 0ml
@13:41:12.45 ethanol wash
@13:41:12.84 ATV.dial :air2
@13:41:14.02 AS.reconfigure ASslow2
@13:41:14.08 AS.seek 0.01ml
@13:41:24.45 delay 3 seconds
@13:41:27.48 Pulling 0.4ml of Ethanol Wash
@13:41:27.49 ATV.dial :ethanol
@13:41:28.38 AS.reconfigure ASslow1
@13:41:28.44 AS.seek 0.41ml
@13:42:49.20 delay 3 seconds
@13:42:52.23 ATV.dial :air1
@13:42:52.65 delay 3 seconds
@13:42:55.72 AS.seek 0.435ml
@13:43:01.04 delay 3 seconds
@13:43:04.07 ATV.dial :ethanol
@13:43:04.71 AS.seek 0.42ml
@13:43:08.02 delay 3 seconds
@13:43:11.05 ATV.dial ASV
@13:43:12.44 AS.seek 0.093ml
@13:44:18.54 delay 3 seconds
@13:44:21.57 ATV.dial :waste2
@13:44:22.38 AS.reconfigure ASconfig
@13:44:22.43 AS.seek 0ml
@13:44:28.10 ATV.dial :air2
@13:44:28.52 AS.reconfigure ASslow1
@13:44:28.57 AS.seek 1ml
@13:47:49.96 delay 5 seconds
@13:47:54.99 ATV.dial ASV
@13:47:56.02 ASV.dial :SPEdelivery
@13:47:56.05 AS.seek 0.02ml
@13:51:13.40 delay 5 seconds
@13:51:18.43 ATV.dial :air2
@13:51:19.54 AS.seek 0.52ml
@13:53:00.37 delay 5 seconds
@13:53:05.40 ATV.dial ASV
@13:53:06.47 ASV.dial :wasteR
@13:53:06.96 AS.seek 0.3ml
@13:53:51.49 delay 20 seconds
@13:54:11.53 AS.seek 0.07ml
@13:54:58.06 delay 20 seconds
@13:55:18.09 ATV.dial :waste2
@13:55:18.89 AS.reconfigure ASconfig
@13:55:18.94 AS.seek 0ml
@13:55:23.26 Elution
@13:55:23.28 ATV.dial :air1
@13:55:24.48 AS.reconfigure ASslow1
@13:55:24.53 AS.seek 0.05ml
@13:55:34.93 delay 3 seconds
@13:55:37.96 ATV.dial :ElutionWater
@13:55:38.67 AS.seek 0.11ml
@13:55:51.01 delay 3 seconds
@13:55:54.04 ATV.dial ASV,via: :air2
@13:55:56.24 ASV.dial :SPEelution
@13:55:56.85 AFV.select :carrierSolution
@13:55:57.01 AS.reconfigure ASnorm
@13:55:57.06 AS.seek 0.76ml
@13:56:30.10 delay 3 seconds
@13:56:33.12 AFV.select :holdingCoil
@13:56:33.27 AS.reconfigure ASslow1
@13:56:33.33 AS.seek 0.475ml
@13:57:30.92 delay 5 seconds
@13:57:36.00 AS.reconfigure ASslow2
@13:57:36.06 AS.seek 0.44ml
@13:58:11.67 delay 1:00
@13:59:11.71 pushing eluate to holding coil
@13:59:11.78 AS.seek 0.28ml
@14:01:53.10 delay 30 seconds
@14:02:23.14 ATV.dial :waste,via: :air3
@14:02:24.99 AS.reconfigure ASconfig
@14:02:25.04 AS.seek 0ml
@14:02:41.45 Parking Bleach in LysateSource line and SPEcolumn
@14:02:41.46 ATV.dial :air3
@14:02:42.37 AS.reconfigure ASslow1
@14:02:42.42 AS.seek 0.03ml
@14:02:48.74 ATV.dial :ElutionWater
@14:02:49.38 AS.seek 0.06ml
@14:02:55.73 ATV.dial :air3
@14:02:56.38 AS.seek 0.07ml
@14:02:58.65 delay 2 seconds
@14:03:00.68 ATV.dial :bleach
@14:03:01.16 AS.seek 0.15ml
@14:03:17.57 delay 3 seconds
@14:03:20.60 ATV.dial :air3
@14:03:21.18 delay 3 seconds
@14:03:24.25 AS.seek 0.175ml
@14:03:29.58 delay 3 seconds
@14:03:32.61 ATV.dial :bleach
@14:03:33.24 AS.seek 0.16ml
@14:03:36.48 delay 3 seconds
@14:03:39.51 ATV.dial :extSrc
@14:03:40.16 AS.reconfigure ASconfig
@14:03:40.21 AS.seek 0.015ml
@14:03:48.85 ATV.dial :waste
@14:03:50.39 AS.seek 0ml
@14:03:51.56 ATV.dial :air3
@14:03:52.46 AS.reconfigure ASslow1
@14:03:52.52 AS.seek 0.01ml
@14:03:54.85 delay 2 seconds
@14:03:56.88 ATV.dial :bleach
@14:03:57.35 AS.seek 0.58ml
@14:05:52.31 delay 3 seconds
@14:05:55.34 ATV.dial :air3
@14:05:55.92 delay 3 seconds
@14:05:58.99 AS.seek 0.605ml
@14:06:04.34 delay 3 seconds
@14:06:07.37 ATV.dial :bleach
@14:06:08.01 AS.seek 0.59ml
@14:06:11.32 delay 3 seconds
@14:06:14.35 ATV.dial :ASV
@14:06:15.45 ASV.dial :wasteR
@14:06:16.08 AS.seek 0ml
@14:08:14.99 delay 1:00
@14:09:15.02 AFV.select :carrierSolution
@14:09:15.13 delay 2 seconds
@14:09:17.15 AS.reconfigure ASconfig
@14:09:17.20 AS.seek 1ml
@14:10:14.96 delay 1 second
@14:10:15.98 AFV.select :holdingCoil
@14:10:16.09 delay 1 second
@14:10:17.11 ATV.dial ASV
@14:10:17.12 ASV.dial :wasteR
@14:10:17.15 AS.reconfigure ASslow1
@14:10:17.21 AS.seek 0ml
@14:13:38.57 delay 20 seconds
@14:13:58.61 ATV.dial :air3
@14:14:00.19 AS.seek 0.59ml
@14:15:59.12 delay 3 seconds
@14:16:02.15 ATV.dial :ASV
@14:16:03.65 ASV.dial :wasteR
@14:16:03.74 AS.seek 0.09ml
@14:17:44.56 delay 30 seconds
@14:18:14.60 ATV.dial :extSrc
@14:18:15.42 delay 3 seconds
@14:18:18.45 ATV.dial :ElutionWater
@14:18:19.63 AS.seek 0.12ml
@14:18:25.96 ATV.dial :air3
@14:18:26.60 AS.seek 0.13ml
@14:18:28.87 delay 2 seconds
@14:18:30.90 ATV.dial :bleach
@14:18:31.38 AS.seek 0.23ml
@14:18:51.74 delay 3 seconds
@14:18:54.77 ATV.dial :air3
@14:18:55.35 delay 3 seconds
@14:18:58.42 AS.seek 0.255ml
@14:19:03.77 delay 3 seconds
@14:19:06.80 ATV.dial :bleach
@14:19:07.43 AS.seek 0.24ml
@14:19:11.06 delay 3 seconds
@14:19:14.16 ATV.dial :ASV
@14:19:15.26 ASV.dial :SPEdelivery
@14:19:15.67 AFV.select :carrierSolution
@14:19:15.79 delay 2 seconds
@14:19:17.82 AS.reconfigure ASconfig
@14:19:17.87 AS.seek 0.5ml
@14:19:33.12 delay 1 second
@14:19:34.14 AFV.select :holdingCoil
@14:19:34.29 AS.reconfigure ASslow1
@14:19:34.35 AS.seek 0.17ml
@14:20:41.02 SPE.hold 2:46:40,95C
@14:20:41.08 delay 3:00
@14:23:41.12 AFV.select :carrierSolution
@14:23:41.24 delay 2 seconds
@14:23:43.26 AS.reconfigure ASconfig
@14:23:43.31 AS.seek 1ml
@14:24:31.31 delay 1 second
@14:24:32.33 AFV.select :holdingCoil
@14:24:32.44 delay 1 second
@14:24:33.46 ASV.dial :wasteD
@14:24:34.58 AS.seek 0ml
@14:25:32.35 Priming Eluate
@14:25:32.36 AFV.select :carrierSolution
@14:25:32.48 delay 2 seconds
@14:25:34.50 AS.seek 0.5ml
@14:26:03.54 delay 1 second
@14:26:04.56 AFV.select :holdingCoil
@14:26:04.67 ATV.dial ASV
@14:26:04.68 ASV.dial :SPErecovery
@14:26:05.32 Swish #1 of 2
@14:26:05.40 AS.reconfigure ASslow0
@14:26:05.46 AS.seek 0.6ml
@14:26:15.93 AS.seek 0.5ml
@14:26:26.35 Swish #2 of 2
@14:26:26.44 AS.seek 0.6ml
@14:26:36.84 AS.seek 0.5ml
@14:26:47.20 delay 3 seconds
@14:26:50.27 AS.reconfigure ASslow1
@14:26:50.33 AS.seek 0.684ml
@14:27:27.67 delay 3 seconds
@14:27:30.70 Eluate primed
@14:27:30.71 ATV.dial :waste2
@14:27:31.38 AS.reconfigure ASnorm
@14:27:31.43 AS.seek 0ml
@14:28:06.26 SPE.coast
@14:28:06.34 ASV.dial :SPErecovery
@14:28:06.36 Setting up PCR for ACYPP2 -- writing /var/log/esp/ACYPP221mar2214h.pcr
@14:28:06.38 Building PCR Slug from ASV with MM1 mix, PP2 primer
@14:28:06.41 Priming with carrier
@14:28:06.42 ATV.dial ARV
@14:28:07.48 ARV.dial :carrier
@14:28:07.84 AS.seek 0.5ml
@14:28:33.42 AFV.select :carrierSolution
@14:28:33.54 delay 2 seconds
@14:28:35.56 AS.reconfigure ASconfig
@14:28:35.63 AS.seek 1ml
@14:29:04.65 delay 1 second
@14:29:05.67 AFV.select :holdingCoil
@14:29:05.78 delay 1 second
@14:29:06.80 ATV.dial :waste
@14:29:08.41 AS.reconfigure ASnorm
@14:29:08.47 AS.seek 0ml
@14:29:59.17 AFV.select :carrierSolution
@14:29:59.30 delay 2 seconds
@14:30:01.32 AS.reconfigure ASconfig
@14:30:01.38 AS.seek 0.5ml
@14:30:30.42 delay 1 second
@14:30:31.44 AFV.select :holdingCoil
@14:30:31.55 Building Slug
@14:30:31.56 ATV.dial :ARV
@14:30:33.32 delay 2 seconds
@14:30:35.35 ARV.dial :air1
@14:30:35.75 delay 2 seconds
@14:30:37.83 AS.reconfigure ASslow2
@14:30:37.88 AS.seek 0.506ml
@14:30:44.07 delay 2 seconds
@14:30:46.10 ARV.dial :MM1
@14:30:46.73 delay 2 seconds
@14:30:48.76 Adding MM1
@14:30:48.83 AS.seek 0.524ml
@14:31:07.15 delay 25 seconds
@14:31:32.18 ARV.dial :PP2
@14:31:32.60 Adding PP2
@14:31:32.61 delay 2 seconds
@14:31:34.69 AS.seek 0.53ml
@14:31:41.01 delay 10 seconds
@14:31:51.05 ARV.dial :air2
@14:31:51.47 adding ARV air
@14:31:51.48 delay 2 seconds
@14:31:53.56 AS.seek 0.536ml
@14:31:59.90 delay 5 seconds
@14:32:04.93 ARV.dial :carrier
@14:32:06.28 delay 7 seconds
@14:32:13.37 AS.seek 0.594ml
@14:33:11.90 delay 5 seconds
@14:33:16.93 ATV.dial :ASV
@14:33:17.35 delay 2 seconds
@14:33:19.43 AS.seek 0.6ml
@14:33:26.04 delay 4 seconds
@14:33:30.07 ATV.dial ARV
@14:33:30.65 delay 2 seconds
@14:33:32.68 ARV.dial :carrier
@14:33:32.69 delay 2 seconds
@14:33:34.77 AS.seek 0.65ml
@14:34:25.46 delay 4 seconds
@14:34:29.49 ATV.dial PCR
@14:34:30.13 ASV.dial :waste1
@14:34:30.96 ARV.dial :air1
@14:34:31.38 Positioning Slug by 0.324ml
@14:34:31.46 AS.seek 0.326ml
@14:39:57.68 delay 5 seconds
@14:40:02.71 ATV.dial ARV
@14:40:03.53 ASV.dial :PCRlock
@14:40:03.92 delay 5 seconds
@14:40:08.95 Running ACYPP2 Profile60CIQ2 thermocycling
@14:40:08.96 <PCR> PCR.program Profile60CIQ2
@14:40:09.08 Profile60CIQ2 should complete in < 1:47:50
@14:40:09.09 PCR.run 2:19:47.5
@16:08:20.03 PCR.off
@16:08:20.65 <MFBPCR> Running ACYPP2 MeltProfile60 thermocycling
@16:08:20.67 <PCR> PCR.program MeltProfile60
@16:08:20.77 MeltProfile60 should complete in < 11:55
@16:08:20.78 PCR.run 19:53.75
@16:15:37.43 PCR.off
@16:15:38.37 <MFBPCR> PCR for ACYPP2 completed -- closed /var/log/esp/ACYPP221mar2214h.pcr
@16:15:38.44 Cleaning PCR
@16:15:38.45 AFV.select :carrierSolution
@16:15:38.58 delay 2 seconds
@16:15:40.60 AS.reconfigure ASconfig
@16:15:40.66 AS.seek 0.5ml
@16:15:50.96 delay 1 second
@16:15:51.98 AFV.select :holdingCoil
@16:15:52.09 ASV.dial :waste1
@16:15:52.48 ATV.dial :air3
@16:15:53.84 AS.reconfigure ASslow1
@16:15:53.90 AS.seek 0.51ml
@16:15:56.23 delay 2 seconds
@16:15:58.26 ATV.dial :bleach
@16:15:58.90 AS.seek 0.81ml
@16:16:59.50 delay 3 seconds
@16:17:02.53 ATV.dial :air3
@16:17:03.11 delay 3 seconds
@16:17:06.18 AS.seek 0.835ml
@16:17:11.54 delay 3 seconds
@16:17:14.57 ATV.dial :bleach
@16:17:15.21 AS.seek 0.82ml
@16:17:18.50 delay 3 seconds
@16:17:21.53 ARV.dial :waste
@16:17:22.17 delay 3 seconds
@16:17:25.20 ATV.dial :ARV
@16:17:26.13 AS.reconfigure ASconfig
@16:17:26.19 AS.seek 0.47ml
@16:17:46.61 AFV.select :carrierSolution
@16:17:46.73 delay 2 seconds
@16:17:48.75 AS.seek 1ml
@16:18:19.50 delay 1 second
@16:18:20.52 AFV.select :holdingCoil
@16:18:20.63 delay 1 second
@16:18:21.65 AS.reconfigure ASfast
@16:18:21.71 AS.seek 0ml
@16:18:49.06 AFV.select :carrierSolution
@16:18:49.18 delay 2 seconds
@16:18:51.20 AS.reconfigure ASconfig
@16:18:51.25 AS.seek 1ml
@16:19:49.01 delay 1 second
@16:19:50.03 AFV.select :holdingCoil
@16:19:50.14 delay 1 second
@16:19:51.16 AS.reconfigure ASfast
@16:19:51.21 AS.seek 0ml
@16:20:18.56 AFV.select :carrierSolution
@16:20:18.68 delay 2 seconds
@16:20:20.70 AS.reconfigure ASconfig
@16:20:20.75 AS.seek 1ml
@16:21:18.50 delay 1 second
@16:21:19.52 AFV.select :holdingCoil
@16:21:19.63 delay 1 second
@16:21:20.65 AS.reconfigure ASfast
@16:21:20.71 AS.seek 0ml
@16:21:48.04 ARV.dial between carrier and air1
@16:21:48.57 ATV.dial :air3
@16:21:49.93 AS.reconfigure ASslow1
@16:21:49.99 AS.seek 0.01ml
@16:21:52.39 delay 2 seconds
@16:21:54.42 ATV.dial :bleach
@16:21:55.06 AS.seek 0.81ml
@16:24:36.31 delay 3 seconds
@16:24:39.34 ATV.dial :air3
@16:24:39.92 delay 3 seconds
@16:24:42.99 AS.seek 0.835ml
@16:24:48.32 delay 3 seconds
@16:24:51.35 ATV.dial :bleach
@16:24:51.99 AS.seek 0.82ml
@16:24:55.26 delay 3 seconds
@16:24:58.29 ATV.dial PCR
@16:24:58.73 AS.reconfigure ASconfig
@16:24:58.78 AS.seek 0ml
@16:25:46.18 PCR.program :bleachProfile
@16:25:46.25 PCR program should complete in < 2:45
@16:25:46.26 PCR.run 10:00
@16:28:42.46 PCR.off
@16:28:42.51 AFV.select :carrierSolution
@16:28:42.62 delay 2 seconds
@16:28:44.64 AS.seek 1ml
@16:29:42.43 delay 1 second
@16:29:43.45 AFV.select :holdingCoil
@16:29:43.56 delay 1 second
@16:29:44.58 AS.reconfigure ASfast
@16:29:44.64 AS.seek 0ml
@16:30:11.98 delay 3 seconds
@16:30:15.00 AFV.select :carrierSolution
@16:30:15.11 delay 2 seconds
@16:30:17.13 AS.reconfigure ASconfig
@16:30:17.19 AS.seek 1ml
@16:31:14.93 delay 1 second
@16:31:15.95 AFV.select :holdingCoil
@16:31:16.06 delay 1 second
@16:31:17.08 AS.reconfigure ASfast
@16:31:17.14 AS.seek 0ml
@16:31:44.47 delay 3 seconds
@16:31:47.49 AFV.select :carrierSolution
@16:31:47.60 delay 2 seconds
@16:31:49.62 AS.reconfigure ASconfig
@16:31:49.68 AS.seek 1ml
@16:32:47.47 delay 1 second
@16:32:48.49 AFV.select :holdingCoil
@16:32:48.60 delay 1 second
@16:32:49.62 AS.reconfigure ASfast
@16:32:49.67 AS.seek 0ml
@16:33:17.03 delay 3 seconds
@16:33:20.05 AFV.select :carrierSolution
@16:33:20.16 delay 2 seconds
@16:33:22.18 AS.reconfigure ASconfig
@16:33:22.24 AS.seek 1ml
@16:34:20.00 delay 1 second
@16:34:21.02 AFV.select :holdingCoil
@16:34:21.13 delay 1 second
@16:34:22.15 AS.reconfigure ASfast
@16:34:22.21 AS.seek 0ml
@16:34:49.56 delay 3 seconds
@16:34:52.58 AFV.select :carrierSolution
@16:34:52.69 delay 2 seconds
@16:34:54.71 AS.reconfigure ASconfig
@16:34:54.77 AS.seek 1ml
@16:35:52.51 delay 1 second
@16:35:53.53 AFV.select :holdingCoil
@16:35:53.64 delay 1 second
@16:35:54.66 AS.reconfigure ASnorm
@16:35:54.71 AS.seek 0ml
@16:36:45.42 Setting up PCR for AOPP1 -- writing /var/log/esp/AOPP121mar2216h.pcr
@16:36:45.43 Building PCR Slug from ASV with MM1 mix, PP1 primer
@16:36:45.46 Priming with carrier
@16:36:45.47 ATV.dial ARV
@16:36:46.22 ARV.dial :carrier
@16:36:46.57 AS.seek 0.5ml
@16:37:12.17 AFV.select :carrierSolution
@16:37:12.29 delay 2 seconds
@16:37:14.31 AS.reconfigure ASconfig
@16:37:14.37 AS.seek 1ml
@16:37:43.37 delay 1 second
@16:37:44.39 AFV.select :holdingCoil
@16:37:44.50 delay 1 second
@16:37:45.52 ATV.dial :waste
@16:37:47.30 AS.reconfigure ASnorm
@16:37:47.36 AS.seek 0ml
@16:38:38.06 AFV.select :carrierSolution
@16:38:38.18 delay 2 seconds
@16:38:40.20 AS.reconfigure ASconfig
@16:38:40.26 AS.seek 0.5ml
@16:39:09.31 delay 1 second
@16:39:10.33 AFV.select :holdingCoil
@16:39:10.44 Building Slug
@16:39:10.45 ATV.dial :ARV
@16:39:12.20 delay 2 seconds
@16:39:14.23 ARV.dial :air1
@16:39:14.63 delay 2 seconds
@16:39:16.70 AS.reconfigure ASslow2
@16:39:16.76 AS.seek 0.506ml
@16:39:23.04 delay 2 seconds
@16:39:25.07 ARV.dial :MM1
@16:39:25.70 delay 2 seconds
@16:39:27.73 Adding MM1
@16:39:27.80 AS.seek 0.524ml
@16:39:46.11 delay 25 seconds
@16:40:11.14 ARV.dial :PP1
@16:40:11.54 Adding PP1
@16:40:11.55 delay 2 seconds
@16:40:13.62 AS.seek 0.53ml
@16:40:19.93 delay 10 seconds
@16:40:29.97 ARV.dial :air1
@16:40:30.38 adding ARV air
@16:40:30.39 delay 2 seconds
@16:40:32.47 AS.seek 0.536ml
@16:40:38.68 delay 5 seconds
@16:40:43.71 ARV.dial :carrier
@16:40:44.12 delay 7 seconds
@16:40:51.20 AS.seek 0.594ml
@16:41:49.78 delay 5 seconds
@16:41:54.81 ATV.dial :ASV
@16:41:55.23 delay 2 seconds
@16:41:57.30 AS.seek 0.6ml
@16:42:03.54 delay 4 seconds
@16:42:07.57 ATV.dial ARV
@16:42:08.14 delay 2 seconds
@16:42:10.17 ARV.dial :carrier
@16:42:10.18 delay 2 seconds
@16:42:12.26 AS.seek 0.65ml
@16:43:02.90 delay 4 seconds
@16:43:06.93 ATV.dial PCR
@16:43:07.58 ASV.dial :waste1
@16:43:07.60 ARV.dial :air1
@16:43:08.02 Positioning Slug by 0.324ml
@16:43:08.10 AS.seek 0.326ml
@16:48:34.29 delay 5 seconds
@16:48:39.32 ATV.dial ARV
@16:48:40.12 ASV.dial :PCRlock
@16:48:40.53 delay 5 seconds
@16:48:45.56 Running AOPP1 Profile60CIQ1 thermocycling
@16:48:45.57 <PCR> PCR.program Profile60CIQ1
@16:48:45.69 Profile60CIQ1 should complete in < 1:56:15
@16:48:45.70 PCR.run 2:30:18.75
@18:27:13.58 PCR.off
@18:27:14.15 <MFBPCR> Running AOPP1 MeltProfile60 thermocycling
@18:27:14.17 <PCR> PCR.program MeltProfile60
@18:27:14.27 MeltProfile60 should complete in < 11:55
@18:27:14.28 PCR.run 19:53.75
@18:34:42.87 PCR.off
@18:34:43.79 <MFBPCR> PCR for AOPP1 completed -- closed /var/log/esp/AOPP121mar2216h.pcr
@18:34:43.87 Cleaning PCR
@18:34:43.88 AFV.select :carrierSolution
@18:34:44.00 delay 2 seconds
@18:34:46.02 AS.reconfigure ASconfig
@18:34:46.07 AS.seek 0.5ml
@18:34:56.38 delay 1 second
@18:34:57.40 AFV.select :holdingCoil
@18:34:57.51 ASV.dial :waste1
@18:34:57.91 ATV.dial :air3
@18:34:59.65 AS.reconfigure ASslow1
@18:34:59.71 AS.seek 0.51ml
@18:35:01.99 delay 2 seconds
@18:35:04.02 ATV.dial :bleach
@18:35:04.65 AS.seek 0.81ml
@18:36:05.23 delay 3 seconds
@18:36:08.26 ATV.dial :air3
@18:36:08.84 delay 3 seconds
@18:36:11.91 AS.seek 0.835ml
@18:36:17.20 delay 3 seconds
@18:36:20.23 ATV.dial :bleach
@18:36:20.87 AS.seek 0.82ml
@18:36:24.20 delay 3 seconds
@18:36:27.23 ARV.dial :waste
@18:36:27.86 delay 3 seconds
@18:36:30.89 ATV.dial :ARV
@18:36:31.83 AS.reconfigure ASconfig
@18:36:31.88 AS.seek 0.47ml
@18:36:52.28 AFV.select :carrierSolution
@18:36:52.40 delay 2 seconds
@18:36:54.42 AS.seek 1ml
@18:37:25.19 delay 1 second
@18:37:26.21 AFV.select :holdingCoil
@18:37:26.32 delay 1 second
@18:37:27.34 AS.reconfigure ASfast
@18:37:27.40 AS.seek 0ml
@18:37:54.75 AFV.select :carrierSolution
@18:37:54.87 delay 2 seconds
@18:37:56.89 AS.reconfigure ASconfig
@18:37:56.94 AS.seek 1ml
@18:38:54.70 delay 1 second
@18:38:55.72 AFV.select :holdingCoil
@18:38:55.83 delay 1 second
@18:38:56.85 AS.reconfigure ASfast
@18:38:56.91 AS.seek 0ml
@18:39:24.27 AFV.select :carrierSolution
@18:39:24.38 delay 2 seconds
@18:39:26.40 AS.reconfigure ASconfig
@18:39:26.45 AS.seek 1ml
@18:40:24.20 delay 1 second
@18:40:25.22 AFV.select :holdingCoil
@18:40:25.33 delay 1 second
@18:40:26.35 AS.reconfigure ASfast
@18:40:26.41 AS.seek 0ml
@18:40:53.77 ARV.dial between carrier and air1
@18:40:54.31 ATV.dial :air3
@18:40:55.65 AS.reconfigure ASslow1
@18:40:55.71 AS.seek 0.01ml
@18:40:58.13 delay 2 seconds
@18:41:00.16 ATV.dial :bleach
@18:41:00.78 AS.seek 0.81ml
@18:43:42.02 delay 3 seconds
@18:43:45.05 ATV.dial :air3
@18:43:45.63 delay 3 seconds
@18:43:48.70 AS.seek 0.835ml
@18:43:54.03 delay 3 seconds
@18:43:57.06 ATV.dial :bleach
@18:43:57.70 AS.seek 0.82ml
@18:44:00.98 delay 3 seconds
@18:44:04.01 ATV.dial PCR
@18:44:04.45 AS.reconfigure ASconfig
@18:44:04.50 AS.seek 0ml
@18:44:51.92 PCR.program :bleachProfile
@18:44:51.99 PCR program should complete in < 2:45
@18:44:52.00 PCR.run 10:00
@18:47:48.21 PCR.off
@18:47:48.25 AFV.select :carrierSolution
@18:47:48.36 delay 2 seconds
@18:47:50.38 AS.seek 1ml
@18:48:48.13 delay 1 second
@18:48:49.15 AFV.select :holdingCoil
@18:48:49.26 delay 1 second
@18:48:50.28 AS.reconfigure ASfast
@18:48:50.34 AS.seek 0ml
@18:49:17.67 delay 3 seconds
@18:49:20.69 AFV.select :carrierSolution
@18:49:20.80 delay 2 seconds
@18:49:22.82 AS.reconfigure ASconfig
@18:49:22.88 AS.seek 1ml
@18:50:20.64 delay 1 second
@18:50:21.66 AFV.select :holdingCoil
@18:50:21.77 delay 1 second
@18:50:22.79 AS.reconfigure ASfast
@18:50:22.85 AS.seek 0ml
@18:50:50.17 delay 3 seconds
@18:50:53.19 AFV.select :carrierSolution
@18:50:53.32 delay 2 seconds
@18:50:55.34 AS.reconfigure ASconfig
@18:50:55.40 AS.seek 1ml
@18:51:53.16 delay 1 second
@18:51:54.18 AFV.select :holdingCoil
@18:51:54.29 delay 1 second
@18:51:55.31 AS.reconfigure ASfast
@18:51:55.37 AS.seek 0ml
@18:52:22.72 delay 3 seconds
@18:52:25.74 AFV.select :carrierSolution
@18:52:25.85 delay 2 seconds
@18:52:27.87 AS.reconfigure ASconfig
@18:52:27.93 AS.seek 1ml
@18:53:25.77 delay 1 second
@18:53:26.79 AFV.select :holdingCoil
@18:53:26.90 delay 1 second
@18:53:27.92 AS.reconfigure ASfast
@18:53:27.98 AS.seek 0ml
@18:53:55.33 delay 3 seconds
@18:53:58.35 AFV.select :carrierSolution
@18:53:58.46 delay 2 seconds
@18:54:00.48 AS.reconfigure ASconfig
@18:54:00.54 AS.seek 1ml
@18:54:58.33 delay 1 second
@18:54:59.35 AFV.select :holdingCoil
@18:54:59.46 delay 1 second
@18:55:00.48 AS.reconfigure ASnorm
@18:55:00.53 AS.seek 0ml
@18:55:51.27 Setting up PCR for AARPP4 -- writing /var/log/esp/AARPP421mar2218h.pcr
@18:55:51.28 Building PCR Slug from ASV with MM2 mix, PP4 primer
@18:55:51.31 Priming with carrier
@18:55:51.33 ATV.dial ARV
@18:55:51.99 ARV.dial :carrier
@18:55:52.34 AS.seek 0.5ml
@18:56:17.89 AFV.select :carrierSolution
@18:56:18.01 delay 2 seconds
@18:56:20.03 AS.reconfigure ASconfig
@18:56:20.09 AS.seek 1ml
@18:56:49.09 delay 1 second
@18:56:50.11 AFV.select :holdingCoil
@18:56:50.22 delay 1 second
@18:56:51.24 ATV.dial :waste
@18:56:53.01 AS.reconfigure ASnorm
@18:56:53.07 AS.seek 0ml
@18:57:43.75 AFV.select :carrierSolution
@18:57:43.88 delay 2 seconds
@18:57:45.90 AS.reconfigure ASconfig
@18:57:45.96 AS.seek 0.5ml
@18:58:15.03 delay 1 second
@18:58:16.05 AFV.select :holdingCoil
@18:58:16.16 Building Slug
@18:58:16.17 ATV.dial :ARV
@18:58:17.91 delay 2 seconds
@18:58:19.94 ARV.dial :air1
@18:58:20.34 delay 2 seconds
@18:58:22.41 AS.reconfigure ASslow2
@18:58:22.47 AS.seek 0.506ml
@18:58:28.77 delay 2 seconds
@18:58:30.80 ARV.dial :MM2
@18:58:32.38 delay 2 seconds
@18:58:34.41 Adding MM2
@18:58:34.48 AS.seek 0.524ml
@18:58:53.00 delay 25 seconds
@18:59:18.03 ARV.dial :PP4
@18:59:18.45 Adding PP4
@18:59:18.46 delay 2 seconds
@18:59:20.53 AS.seek 0.53ml
@18:59:26.89 delay 10 seconds
@18:59:36.93 ARV.dial :air3
@18:59:37.35 adding ARV air
@18:59:37.36 delay 2 seconds
@18:59:39.43 AS.seek 0.536ml
@18:59:46.13 delay 5 seconds
@18:59:51.16 ARV.dial :carrier
@18:59:52.47 delay 7 seconds
@18:59:59.55 AS.seek 0.594ml
@19:00:58.36 delay 5 seconds
@19:01:03.39 ATV.dial :ASV
@19:01:03.81 delay 2 seconds
@19:01:05.88 AS.seek 0.6ml
@19:01:12.45 delay 4 seconds
@19:01:16.48 ATV.dial ARV
@19:01:17.05 delay 2 seconds
@19:01:19.08 ARV.dial :carrier
@19:01:19.09 delay 2 seconds
@19:01:21.16 AS.seek 0.65ml
@19:02:11.92 delay 4 seconds
@19:02:15.95 ATV.dial PCR
@19:02:16.59 ASV.dial :waste1
@19:02:16.61 ARV.dial :air1
@19:02:17.03 Positioning Slug by 0.324ml
@19:02:17.12 AS.seek 0.326ml
@19:07:43.22 delay 5 seconds
@19:07:48.25 ATV.dial ARV
@19:07:49.48 ASV.dial :PCRlock
@19:07:49.88 delay 5 seconds
@19:07:54.91 Running AARPP4 Profile58CIQ2 thermocycling
@19:07:54.92 <PCR> PCR.program Profile58CIQ2
@19:07:55.04 Profile58CIQ2 should complete in < 1:36:15
@19:07:55.05 PCR.run 2:05:18.75
@20:28:05.41 PCR.off
@20:28:05.98 <MFBPCR> Running AARPP4 MeltProfile58 thermocycling
@20:28:05.99 <PCR> PCR.program MeltProfile58
@20:28:06.10 MeltProfile58 should complete in < 12:33
@20:28:06.11 PCR.run 20:41.25
@20:35:36.39 PCR.off
@20:35:37.76 <MFBPCR> PCR for AARPP4 completed -- closed /var/log/esp/AARPP421mar2218h.pcr
@20:35:37.84 Cleaning PCR
@20:35:37.85 AFV.select :carrierSolution
@20:35:37.97 delay 2 seconds
@20:35:39.99 AS.reconfigure ASconfig
@20:35:40.05 AS.seek 0.5ml
@20:35:50.32 delay 1 second
@20:35:51.34 AFV.select :holdingCoil
@20:35:51.45 ASV.dial :waste1
@20:35:51.83 ATV.dial :air3
@20:35:53.19 AS.reconfigure ASslow1
@20:35:53.25 AS.seek 0.51ml
@20:35:55.53 delay 2 seconds
@20:35:57.56 ATV.dial :bleach
@20:35:58.21 AS.seek 0.81ml
@20:36:58.80 delay 3 seconds
@20:37:01.83 ATV.dial :air3
@20:37:02.41 delay 3 seconds
@20:37:05.48 AS.seek 0.835ml
@20:37:10.78 delay 3 seconds
@20:37:13.81 ATV.dial :bleach
@20:37:14.46 AS.seek 0.82ml
@20:37:17.77 delay 3 seconds
@20:37:20.80 ARV.dial :waste
@20:37:21.43 delay 3 seconds
@20:37:24.46 ATV.dial :ARV
@20:37:25.39 AS.reconfigure ASconfig
@20:37:25.45 AS.seek 0.47ml
@20:37:45.83 AFV.select :carrierSolution
@20:37:45.95 delay 2 seconds
@20:37:47.97 AS.seek 1ml
@20:38:18.74 delay 1 second
@20:38:19.76 AFV.select :holdingCoil
@20:38:19.87 delay 1 second
@20:38:20.89 AS.reconfigure ASfast
@20:38:20.95 AS.seek 0ml
@20:38:48.30 AFV.select :carrierSolution
@20:38:48.42 delay 2 seconds
@20:38:50.44 AS.reconfigure ASconfig
@20:38:50.49 AS.seek 1ml
@20:39:48.24 delay 1 second
@20:39:49.26 AFV.select :holdingCoil
@20:39:49.37 delay 1 second
@20:39:50.39 AS.reconfigure ASfast
@20:39:50.45 AS.seek 0ml
@20:40:17.79 AFV.select :carrierSolution
@20:40:17.91 delay 2 seconds
@20:40:19.93 AS.reconfigure ASconfig
@20:40:19.98 AS.seek 1ml
@20:41:17.77 delay 1 second
@20:41:18.79 AFV.select :holdingCoil
@20:41:18.90 delay 1 second
@20:41:19.92 AS.reconfigure ASfast
@20:41:19.98 AS.seek 0ml
@20:41:47.30 ARV.dial between carrier and air1
@20:41:47.83 ATV.dial :air3
@20:41:49.19 AS.reconfigure ASslow1
@20:41:49.25 AS.seek 0.01ml
@20:41:51.55 delay 2 seconds
@20:41:53.58 ATV.dial :bleach
@20:41:54.22 AS.seek 0.81ml
@20:44:35.54 delay 3 seconds
@20:44:38.57 ATV.dial :air3
@20:44:39.15 delay 3 seconds
@20:44:42.22 AS.seek 0.835ml
@20:44:47.56 delay 3 seconds
@20:44:50.59 ATV.dial :bleach
@20:44:51.22 AS.seek 0.82ml
@20:44:54.54 delay 3 seconds
@20:44:57.57 ATV.dial PCR
@20:44:58.00 AS.reconfigure ASconfig
@20:44:58.05 AS.seek 0ml
@20:45:45.47 PCR.program :bleachProfile
@20:45:45.54 PCR program should complete in < 2:45
@20:45:45.55 PCR.run 10:00
@20:48:44.78 PCR.off
@20:48:44.82 AFV.select :carrierSolution
@20:48:44.93 delay 2 seconds
@20:48:46.95 AS.seek 1ml
@20:49:44.74 delay 1 second
@20:49:45.76 AFV.select :holdingCoil
@20:49:45.87 delay 1 second
@20:49:46.89 AS.reconfigure ASfast
@20:49:46.95 AS.seek 0ml
@20:50:14.30 delay 3 seconds
@20:50:17.32 AFV.select :carrierSolution
@20:50:17.43 delay 2 seconds
@20:50:19.45 AS.reconfigure ASconfig
@20:50:19.52 AS.seek 1ml
@20:51:17.29 delay 1 second
@20:51:18.31 AFV.select :holdingCoil
@20:51:18.42 delay 1 second
@20:51:19.44 AS.reconfigure ASfast
@20:51:19.50 AS.seek 0ml
@20:51:46.82 delay 3 seconds
@20:51:49.84 AFV.select :carrierSolution
@20:51:49.95 delay 2 seconds
@20:51:51.97 AS.reconfigure ASconfig
@20:51:52.03 AS.seek 1ml
@20:52:49.82 delay 1 second
@20:52:50.84 AFV.select :holdingCoil
@20:52:50.95 delay 1 second
@20:52:51.97 AS.reconfigure ASfast
@20:52:52.03 AS.seek 0ml
@20:53:19.36 delay 3 seconds
@20:53:22.38 AFV.select :carrierSolution
@20:53:22.49 delay 2 seconds
@20:53:24.51 AS.reconfigure ASconfig
@20:53:24.57 AS.seek 1ml
@20:54:22.36 delay 1 second
@20:54:23.38 AFV.select :holdingCoil
@20:54:23.49 delay 1 second
@20:54:24.51 AS.reconfigure ASfast
@20:54:24.57 AS.seek 0ml
@20:54:51.93 delay 3 seconds
@20:54:54.95 AFV.select :carrierSolution
@20:54:55.06 delay 2 seconds
@20:54:57.08 AS.reconfigure ASconfig
@20:54:57.13 AS.seek 1ml
@20:55:54.93 delay 1 second
@20:55:55.95 AFV.select :holdingCoil
@20:55:56.06 delay 1 second
@20:55:57.08 AS.reconfigure ASnorm
@20:55:57.14 AS.seek 0ml
@20:56:47.89 Setting up PCR for AALPP3 -- writing /var/log/esp/AALPP321mar2220h.pcr
@20:56:47.90 Building PCR Slug from ASV with MM2 mix, PP3 primer
@20:56:47.93 Priming with carrier
@20:56:47.94 ATV.dial ARV
@20:56:48.60 ARV.dial :carrier
@20:56:48.96 AS.seek 0.5ml
@20:57:14.54 AFV.select :carrierSolution
@20:57:14.66 delay 2 seconds
@20:57:16.68 AS.reconfigure ASconfig
@20:57:16.74 AS.seek 1ml
@20:57:45.74 delay 1 second
@20:57:46.76 AFV.select :holdingCoil
@20:57:46.87 delay 1 second
@20:57:47.89 ATV.dial :waste
@20:57:49.67 AS.reconfigure ASnorm
@20:57:49.73 AS.seek 0ml
@20:58:40.40 AFV.select :carrierSolution
@20:58:40.52 delay 2 seconds
@20:58:42.54 AS.reconfigure ASconfig
@20:58:42.60 AS.seek 0.5ml
@20:59:11.66 delay 1 second
@20:59:12.68 AFV.select :holdingCoil
@20:59:12.79 Building Slug
@20:59:12.80 ATV.dial :ARV
@20:59:14.54 delay 2 seconds
@20:59:16.57 ARV.dial :air1
@20:59:16.97 delay 2 seconds
@20:59:19.04 AS.reconfigure ASslow2
@20:59:19.10 AS.seek 0.506ml
@20:59:25.49 delay 2 seconds
@20:59:27.52 ARV.dial :MM2
@20:59:29.08 delay 2 seconds
@20:59:31.10 Adding MM2
@20:59:31.17 AS.seek 0.524ml
@20:59:49.38 delay 25 seconds
@21:00:14.41 ARV.dial :PP3
@21:00:14.80 Adding PP3
@21:00:14.82 delay 2 seconds
@21:00:16.89 AS.seek 0.53ml
@21:00:23.10 delay 10 seconds
@21:00:33.14 ARV.dial :air2
@21:00:33.55 adding ARV air
@21:00:33.57 delay 2 seconds
@21:00:35.64 AS.seek 0.536ml
@21:00:42.07 delay 5 seconds
@21:00:47.10 ARV.dial :carrier
@21:00:48.43 delay 7 seconds
@21:00:55.51 AS.seek 0.594ml
@21:01:54.08 delay 5 seconds
@21:01:59.11 ATV.dial :ASV
@21:01:59.54 delay 2 seconds
@21:02:01.61 AS.seek 0.6ml
@21:02:07.85 delay 4 seconds
@21:02:11.88 ATV.dial ARV
@21:02:12.44 delay 2 seconds
@21:02:14.47 ARV.dial :carrier
@21:02:14.48 delay 2 seconds
@21:02:16.56 AS.seek 0.65ml
@21:03:07.07 delay 4 seconds
@21:03:11.10 ATV.dial PCR
@21:03:11.76 ASV.dial :waste1
@21:03:11.78 ARV.dial :air1
@21:03:12.20 Positioning Slug by 0.324ml
@21:03:12.28 AS.seek 0.326ml
@21:08:38.51 delay 5 seconds
@21:08:43.54 ATV.dial ARV
@21:08:44.37 ASV.dial :PCRlock
@21:08:44.77 delay 5 seconds
@21:08:49.80 Running AALPP3 Profile58CIQ2 thermocycling
@21:08:49.81 <PCR> PCR.program Profile58CIQ2
@21:08:49.93 Profile58CIQ2 should complete in < 1:36:15
@21:08:49.94 PCR.run 2:05:18.75
@22:28:54.40 PCR.off
@22:28:55.34 <MFBPCR> Running AALPP3 MeltProfile58 thermocycling
@22:28:55.35 <PCR> PCR.program MeltProfile58
@22:28:55.45 MeltProfile58 should complete in < 12:33
@22:28:55.47 PCR.run 20:41.25
@22:36:25.92 PCR.off
@22:36:26.89 <MFBPCR> PCR for AALPP3 completed -- closed /var/log/esp/AALPP321mar2220h.pcr
@22:36:26.97 Cleaning PCR
@22:36:26.98 AFV.select :carrierSolution
@22:36:27.10 delay 2 seconds
@22:36:29.12 AS.reconfigure ASconfig
@22:36:29.18 AS.seek 0.5ml
@22:36:39.46 delay 1 second
@22:36:40.48 AFV.select :holdingCoil
@22:36:40.59 ASV.dial :waste1
@22:36:40.98 ATV.dial :air3
@22:36:42.35 AS.reconfigure ASslow1
@22:36:42.41 AS.seek 0.51ml
@22:36:44.74 delay 2 seconds
@22:36:46.77 ATV.dial :bleach
@22:36:47.41 AS.seek 0.81ml
@22:37:47.98 delay 3 seconds
@22:37:51.01 ATV.dial :air3
@22:37:51.59 delay 3 seconds
@22:37:54.66 AS.seek 0.835ml
@22:37:59.97 delay 3 seconds
@22:38:03.00 ATV.dial :bleach
@22:38:03.63 AS.seek 0.82ml
@22:38:06.95 delay 3 seconds
@22:38:09.98 ARV.dial :waste
@22:38:10.60 delay 3 seconds
@22:38:13.63 ATV.dial :ARV
@22:38:14.57 AS.reconfigure ASconfig
@22:38:14.62 AS.seek 0.47ml
@22:38:35.06 AFV.select :carrierSolution
@22:38:35.18 delay 2 seconds
@22:38:37.21 AS.seek 1ml
@22:39:07.98 delay 1 second
@22:39:09.00 AFV.select :holdingCoil
@22:39:09.11 delay 1 second
@22:39:10.13 AS.reconfigure ASfast
@22:39:10.19 AS.seek 0ml
@22:39:37.53 AFV.select :carrierSolution
@22:39:37.65 delay 2 seconds
@22:39:39.67 AS.reconfigure ASconfig
@22:39:39.73 AS.seek 1ml
@22:40:37.48 delay 1 second
@22:40:38.50 AFV.select :holdingCoil
@22:40:38.61 delay 1 second
@22:40:39.63 AS.reconfigure ASfast
@22:40:39.69 AS.seek 0ml
@22:41:07.04 AFV.select :carrierSolution
@22:41:07.16 delay 2 seconds
@22:41:09.18 AS.reconfigure ASconfig
@22:41:09.23 AS.seek 1ml
@22:42:06.99 delay 1 second
@22:42:08.01 AFV.select :holdingCoil
@22:42:08.12 delay 1 second
@22:42:09.14 AS.reconfigure ASfast
@22:42:09.20 AS.seek 0ml
@22:42:36.53 ARV.dial between carrier and air1
@22:42:37.04 ATV.dial :air3
@22:42:38.41 AS.reconfigure ASslow1
@22:42:38.47 AS.seek 0.01ml
@22:42:40.87 delay 2 seconds
@22:42:42.90 ATV.dial :bleach
@22:42:43.54 AS.seek 0.81ml
@22:45:24.70 delay 3 seconds
@22:45:27.73 ATV.dial :air3
@22:45:28.31 delay 3 seconds
@22:45:31.38 AS.seek 0.835ml
@22:45:36.74 delay 3 seconds
@22:45:39.77 ATV.dial :bleach
@22:45:40.41 AS.seek 0.82ml
@22:45:43.70 delay 3 seconds
@22:45:46.73 ATV.dial PCR
@22:45:47.16 AS.reconfigure ASconfig
@22:45:47.21 AS.seek 0ml
@22:46:34.62 PCR.program :bleachProfile
@22:46:34.69 PCR program should complete in < 2:45
@22:46:34.70 PCR.run 10:00
@22:49:33.94 PCR.off
@22:49:33.98 AFV.select :carrierSolution
@22:49:34.09 delay 2 seconds
@22:49:36.11 AS.seek 1ml
@22:50:33.87 delay 1 second
@22:50:34.89 AFV.select :holdingCoil
@22:50:35.00 delay 1 second
@22:50:36.02 AS.reconfigure ASfast
@22:50:36.08 AS.seek 0ml
@22:51:03.40 delay 3 seconds
@22:51:06.42 AFV.select :carrierSolution
@22:51:06.53 delay 2 seconds
@22:51:08.55 AS.reconfigure ASconfig
@22:51:08.62 AS.seek 1ml
@22:52:06.40 delay 1 second
@22:52:07.42 AFV.select :holdingCoil
@22:52:07.53 delay 1 second
@22:52:08.55 AS.reconfigure ASfast
@22:52:08.61 AS.seek 0ml
@22:52:35.95 delay 3 seconds
@22:52:38.97 AFV.select :carrierSolution
@22:52:39.08 delay 2 seconds
@22:52:41.10 AS.reconfigure ASconfig
@22:52:41.15 AS.seek 1ml
@22:53:38.89 delay 1 second
@22:53:39.91 AFV.select :holdingCoil
@22:53:40.02 delay 1 second
@22:53:41.04 AS.reconfigure ASfast
@22:53:41.10 AS.seek 0ml
@22:54:08.43 delay 3 seconds
@22:54:11.45 AFV.select :carrierSolution
@22:54:11.56 delay 2 seconds
@22:54:13.58 AS.reconfigure ASconfig
@22:54:13.64 AS.seek 1ml
@22:55:11.40 delay 1 second
@22:55:12.42 AFV.select :holdingCoil
@22:55:12.53 delay 1 second
@22:55:13.55 AS.reconfigure ASfast
@22:55:13.61 AS.seek 0ml
@22:55:40.95 delay 3 seconds
@22:55:43.97 AFV.select :carrierSolution
@22:55:44.08 delay 2 seconds
@22:55:46.10 AS.reconfigure ASconfig
@22:55:46.15 AS.seek 1ml
@22:56:43.90 delay 1 second
@22:56:44.92 AFV.select :holdingCoil
@22:56:45.03 delay 1 second
@22:56:46.05 AS.reconfigure ASnorm
@22:56:46.10 AS.seek 0ml
@22:57:36.84 Setting up PCR for ACOPP5 -- writing /var/log/esp/ACOPP521mar2222h.pcr
@22:57:36.85 Building PCR Slug from ASV with MM3 mix, PP5 primer
@22:57:36.88 Priming with carrier
@22:57:36.89 ATV.dial ARV
@22:57:37.56 ARV.dial :carrier
@22:57:37.91 AS.seek 0.5ml
@22:58:03.45 AFV.select :carrierSolution
@22:58:03.57 delay 2 seconds
@22:58:05.59 AS.reconfigure ASconfig
@22:58:05.65 AS.seek 1ml
@22:58:34.67 delay 1 second
@22:58:35.69 AFV.select :holdingCoil
@22:58:35.80 delay 1 second
@22:58:36.82 ATV.dial :waste
@22:58:38.60 AS.reconfigure ASnorm
@22:58:38.66 AS.seek 0ml
@22:59:29.32 AFV.select :carrierSolution
@22:59:29.44 delay 2 seconds
@22:59:31.46 AS.reconfigure ASconfig
@22:59:31.52 AS.seek 0.5ml
@23:00:00.54 delay 1 second
@23:00:01.56 AFV.select :holdingCoil
@23:00:01.67 Building Slug
@23:00:01.68 ATV.dial :ARV
@23:00:03.42 delay 2 seconds
@23:00:05.45 ARV.dial :air1
@23:00:05.85 delay 2 seconds
@23:00:07.92 AS.reconfigure ASslow2
@23:00:07.98 AS.seek 0.506ml
@23:00:14.28 delay 2 seconds
@23:00:16.70 ARV.dial :MM3
@23:00:17.79 delay 2 seconds
@23:00:19.82 Adding MM3
@23:00:19.90 AS.seek 0.524ml
@23:00:38.20 delay 25 seconds
@23:01:03.23 ARV.dial :PP5
@23:01:03.65 Adding PP5
@23:01:03.66 delay 2 seconds
@23:01:05.73 AS.seek 0.53ml
@23:01:12.53 delay 10 seconds
@23:01:22.57 ARV.dial :air3
@23:01:22.97 adding ARV air
@23:01:22.98 delay 2 seconds
@23:01:25.05 AS.seek 0.536ml
@23:01:31.45 delay 5 seconds
@23:01:36.48 ARV.dial :carrier
@23:01:37.79 delay 7 seconds
@23:01:44.87 AS.seek 0.594ml
@23:02:43.54 delay 5 seconds
@23:02:48.57 ATV.dial :ASV
@23:02:49.00 delay 2 seconds
@23:02:51.07 AS.seek 0.6ml
@23:02:57.43 delay 4 seconds
@23:03:01.46 ATV.dial ARV
@23:03:02.04 delay 2 seconds
@23:03:04.07 ARV.dial :carrier
@23:03:04.08 delay 2 seconds
@23:03:06.16 AS.seek 0.65ml
@23:03:56.64 delay 4 seconds
@23:04:00.67 ATV.dial PCR
@23:04:01.32 ASV.dial :waste1
@23:04:01.34 ARV.dial :air1
@23:04:01.78 Positioning Slug by 0.324ml
@23:04:01.86 AS.seek 0.326ml
@23:09:27.67 delay 5 seconds
@23:09:32.70 ATV.dial ARV
@23:09:33.53 ASV.dial :PCRlock
@23:09:33.93 delay 5 seconds
@23:09:38.96 Running ACOPP5 ProfileIPc thermocycling
@23:09:38.97 <PCR> PCR.program ProfileIPc
@23:09:39.10 ProfileIPc should complete in < 1:26:35
@23:09:39.11 PCR.run 1:53:13.75
@00:17:38.57CET23-Mar-21 PCR.off
@00:17:39.12 <MFBPCR> PCR for ACOPP5 completed -- closed /var/log/esp/ACOPP521mar2222h.pcr
@00:17:39.20 Cleaning PCR
@00:17:39.21 AFV.select :carrierSolution
@00:17:39.33 delay 2 seconds
@00:17:41.35 AS.reconfigure ASconfig
@00:17:41.40 AS.seek 0.5ml
@00:17:51.70 delay 1 second
@00:17:52.72 AFV.select :holdingCoil
@00:17:52.83 ASV.dial :waste1
@00:17:53.22 ATV.dial :air3
@00:17:54.59 AS.reconfigure ASslow1
@00:17:54.65 AS.seek 0.51ml
@00:17:56.95 delay 2 seconds
@00:17:58.98 ATV.dial :bleach
@00:17:59.62 AS.seek 0.81ml
@00:19:00.26 delay 3 seconds
@00:19:03.29 ATV.dial :air3
@00:19:03.87 delay 3 seconds
@00:19:06.94 AS.seek 0.835ml
@00:19:12.28 delay 3 seconds
@00:19:15.31 ATV.dial :bleach
@00:19:15.95 AS.seek 0.82ml
@00:19:19.26 delay 3 seconds
@00:19:22.29 ARV.dial :waste
@00:19:22.92 delay 3 seconds
@00:19:25.95 ATV.dial :ARV
@00:19:26.89 AS.reconfigure ASconfig
@00:19:26.95 AS.seek 0.47ml
@00:19:47.36 AFV.select :carrierSolution
@00:19:47.48 delay 2 seconds
@00:19:49.50 AS.seek 1ml
@00:20:20.25 delay 1 second
@00:20:21.27 AFV.select :holdingCoil
@00:20:21.38 delay 1 second
@00:20:22.40 AS.reconfigure ASfast
@00:20:22.46 AS.seek 0ml
@00:20:49.80 AFV.select :carrierSolution
@00:20:49.92 delay 2 seconds
@00:20:51.94 AS.reconfigure ASconfig
@00:20:51.99 AS.seek 1ml
@00:21:49.75 delay 1 second
@00:21:50.77 AFV.select :holdingCoil
@00:21:50.88 delay 1 second
@00:21:51.90 AS.reconfigure ASfast
@00:21:51.96 AS.seek 0ml
@00:22:19.33 AFV.select :carrierSolution
@00:22:19.45 delay 2 seconds
@00:22:21.47 AS.reconfigure ASconfig
@00:22:21.52 AS.seek 1ml
@00:23:19.31 delay 1 second
@00:23:20.33 AFV.select :holdingCoil
@00:23:20.44 delay 1 second
@00:23:21.46 AS.reconfigure ASfast
@00:23:21.52 AS.seek 0ml
@00:23:48.86 ARV.dial between carrier and air1
@00:23:49.37 ATV.dial :air3
@00:23:50.73 AS.reconfigure ASslow1
@00:23:50.79 AS.seek 0.01ml
@00:23:53.19 delay 2 seconds
@00:23:55.22 ATV.dial :bleach
@00:23:55.86 AS.seek 0.81ml
@00:26:37.05 delay 3 seconds
@00:26:40.08 ATV.dial :air3
@00:26:40.66 delay 3 seconds
@00:26:43.73 AS.seek 0.835ml
@00:26:49.06 delay 3 seconds
@00:26:52.08 ATV.dial :bleach
@00:26:52.72 AS.seek 0.82ml
@00:26:56.01 delay 3 seconds
@00:26:59.04 ATV.dial PCR
@00:26:59.46 AS.reconfigure ASconfig
@00:26:59.51 AS.seek 0ml
@00:27:46.92 PCR.program :bleachProfile
@00:27:46.99 PCR program should complete in < 2:45
@00:27:47.00 PCR.run 10:00
@00:30:46.24 PCR.off
@00:30:46.28 AFV.select :carrierSolution
@00:30:46.39 delay 2 seconds
@00:30:48.41 AS.seek 1ml
@00:31:46.17 delay 1 second
@00:31:47.19 AFV.select :holdingCoil
@00:31:47.30 delay 1 second
@00:31:48.32 AS.reconfigure ASfast
@00:31:48.38 AS.seek 0ml
@00:32:15.72 delay 3 seconds
@00:32:18.74 AFV.select :carrierSolution
@00:32:18.85 delay 2 seconds
@00:32:20.87 AS.reconfigure ASconfig
@00:32:20.94 AS.seek 1ml
@00:33:18.69 delay 1 second
@00:33:19.71 AFV.select :holdingCoil
@00:33:19.82 delay 1 second
@00:33:20.84 AS.reconfigure ASfast
@00:33:20.90 AS.seek 0ml
@00:33:48.25 delay 3 seconds
@00:33:51.27 AFV.select :carrierSolution
@00:33:51.38 delay 2 seconds
@00:33:53.40 AS.reconfigure ASconfig
@00:33:53.47 AS.seek 1ml
@00:34:51.22 delay 1 second
@00:34:52.24 AFV.select :holdingCoil
@00:34:52.35 delay 1 second
@00:34:53.37 AS.reconfigure ASfast
@00:34:53.43 AS.seek 0ml
@00:35:20.78 delay 3 seconds
@00:35:23.80 AFV.select :carrierSolution
@00:35:23.91 delay 2 seconds
@00:35:25.93 AS.reconfigure ASconfig
@00:35:25.99 AS.seek 1ml
@00:36:23.75 delay 1 second
@00:36:24.77 AFV.select :holdingCoil
@00:36:24.88 delay 1 second
@00:36:25.90 AS.reconfigure ASfast
@00:36:25.96 AS.seek 0ml
@00:36:53.30 delay 3 seconds
@00:36:56.32 AFV.select :carrierSolution
@00:36:56.43 delay 2 seconds
@00:36:58.45 AS.reconfigure ASconfig
@00:36:58.51 AS.seek 1ml
@00:37:56.28 delay 1 second
@00:37:57.30 AFV.select :holdingCoil
@00:37:57.41 delay 1 second
@00:37:58.43 AS.reconfigure ASnorm
@00:37:58.48 AS.seek 0ml
@00:38:49.20 Setting up PCR for AIPc -- writing /var/log/esp/AIPc21mar2300h.pcr
@00:38:49.21 Building PCR Slug from ASV with MM3 mix, PP6 primer
@00:38:49.23 Priming with carrier
@00:38:49.26 ATV.dial ARV
@00:38:49.91 ARV.dial :carrier
@00:38:50.26 AS.seek 0.5ml
@00:39:15.78 AFV.select :carrierSolution
@00:39:15.90 delay 2 seconds
@00:39:17.92 AS.reconfigure ASconfig
@00:39:17.98 AS.seek 1ml
@00:39:46.98 delay 1 second
@00:39:48.00 AFV.select :holdingCoil
@00:39:48.11 delay 1 second
@00:39:49.13 ATV.dial :waste
@00:39:50.92 AS.reconfigure ASnorm
@00:39:50.98 AS.seek 0ml
@00:40:41.66 AFV.select :carrierSolution
@00:40:41.78 delay 2 seconds
@00:40:43.80 AS.reconfigure ASconfig
@00:40:43.86 AS.seek 0.5ml
@00:41:12.89 delay 1 second
@00:41:13.91 AFV.select :holdingCoil
@00:41:14.02 Building Slug
@00:41:14.03 ATV.dial :ARV
@00:41:15.78 delay 2 seconds
@00:41:17.81 ARV.dial :air1
@00:41:18.20 delay 2 seconds
@00:41:20.27 AS.reconfigure ASslow2
@00:41:20.33 AS.seek 0.506ml
@00:41:26.81 delay 2 seconds
@00:41:28.84 ARV.dial :MM3
@00:41:30.33 delay 2 seconds
@00:41:32.36 Adding MM3
@00:41:32.43 AS.seek 0.524ml
@00:41:50.83 delay 25 seconds
@00:42:15.86 ARV.dial :PP6
@00:42:16.26 Adding PP6
@00:42:16.27 delay 2 seconds
@00:42:18.34 AS.seek 0.53ml
@00:42:24.64 delay 10 seconds
@00:42:34.68 ARV.dial :air1
@00:42:35.53 adding ARV air
@00:42:35.55 delay 2 seconds
@00:42:37.62 AS.seek 0.536ml
@00:42:44.33 delay 5 seconds
@00:42:49.36 ARV.dial :carrier
@00:42:49.77 delay 7 seconds
@00:42:56.85 AS.seek 0.594ml
@00:43:55.31 delay 5 seconds
@00:44:00.34 ATV.dial :ASV
@00:44:00.75 delay 2 seconds
@00:44:02.82 AS.seek 0.6ml
@00:44:09.02 delay 4 seconds
@00:44:13.05 ATV.dial ARV
@00:44:13.73 delay 2 seconds
@00:44:15.76 ARV.dial :carrier
@00:44:15.77 delay 2 seconds
@00:44:17.85 AS.seek 0.65ml
@00:45:08.27 delay 4 seconds
@00:45:12.30 ATV.dial PCR
@00:45:12.94 ASV.dial :waste1
@00:45:12.96 ARV.dial :air1
@00:45:13.37 Positioning Slug by 0.324ml
@00:45:13.46 AS.seek 0.326ml
@00:50:39.47 delay 5 seconds
@00:50:44.50 ATV.dial ARV
@00:50:45.31 ASV.dial :PCRlock
@00:50:45.72 delay 5 seconds
@00:50:50.75 Running AIPc ProfileIPc thermocycling
@00:50:50.76 <PCR> PCR.program ProfileIPc
@00:50:50.88 ProfileIPc should complete in < 1:26:35
@00:50:50.89 PCR.run 1:53:13.75
@01:58:51.59 PCR.off
@01:58:52.16 <MFBPCR> PCR for AIPc completed -- closed /var/log/esp/AIPc21mar2300h.pcr
@01:58:52.18 Full MFB Decontamination
@01:58:52.20 Decon extraction column and decon extSrc
@01:58:52.23 SPE.hold 2:46:40,55C
@01:58:52.28 ASV.dial :SPEdelivery
@01:58:52.96 delay 20 seconds
@01:59:12.99 AFV.select :carrierSolution
@01:59:13.10 delay 2 seconds
@01:59:15.12 AS.reconfigure ASconfig
@01:59:15.17 AS.seek 0.5ml
@01:59:25.47 delay 1 second
@01:59:26.49 AFV.select :holdingCoil
@01:59:26.60 ATV.dial :air1
@01:59:28.87 AS.reconfigure ASslow1
@01:59:28.93 AS.seek 0.52ml
@01:59:33.22 ATV.dial :ASV
@01:59:34.99 ASV.dial :SPErecovery
@01:59:35.69 AS.seek 0.62ml
@01:59:56.10 ATV.dial :waste
@01:59:58.33 AS.reconfigure ASnorm
@01:59:58.39 AS.seek 0ml
@02:00:29.96 deconEluateRecoveryLine extSrc
@02:00:29.97 ATV.dial :waste
@02:00:30.00 AS.seek 0ml
@02:00:30.25 ATV.dial :air3
@02:00:31.02 AS.reconfigure ASslow1
@02:00:31.08 AS.seek 0.01ml
@02:00:33.38 delay 2 seconds
@02:00:35.41 ATV.dial :bleach
@02:00:35.91 AS.seek 0.31ml
@02:01:36.52 delay 3 seconds
@02:01:39.55 ATV.dial :air3
@02:01:40.16 delay 3 seconds
@02:01:43.23 AS.seek 0.335ml
@02:01:48.54 delay 3 seconds
@02:01:51.57 ATV.dial :bleach
@02:01:52.24 AS.seek 0.32ml
@02:01:55.55 delay 3 seconds
@02:01:58.58 ATV.dial :extSrc
@02:01:59.26 AS.seek 0ml
@02:03:03.96 AFV.select :carrierSolution
@02:03:04.08 delay 2 seconds
@02:03:06.10 AS.reconfigure ASconfig
@02:03:06.16 AS.seek 0.5ml
@02:03:35.18 delay 1 second
@02:03:36.20 AFV.select :holdingCoil
@02:03:36.31 ATV.dial :extSrc
@02:03:36.33 AS.reconfigure ASslow1
@02:03:36.39 AS.seek 0ml
@02:05:17.22 AFV.select :carrierSolution
@02:05:17.34 delay 2 seconds
@02:05:19.36 AS.reconfigure ASconfig
@02:05:19.42 AS.seek 0.5ml
@02:05:48.46 delay 1 second
@02:05:49.48 AFV.select :holdingCoil
@02:05:49.59 ATV.dial :extSrc
@02:05:49.61 AS.reconfigure ASslow1
@02:05:49.67 AS.seek 0ml
@02:07:30.58 AFV.select :carrierSolution
@02:07:30.70 delay 2 seconds
@02:07:32.72 AS.reconfigure ASconfig
@02:07:32.78 AS.seek 0.5ml
@02:08:01.80 delay 1 second
@02:08:02.82 AFV.select :holdingCoil
@02:08:02.93 ATV.dial :extSrc
@02:08:02.95 AS.reconfigure ASslow1
@02:08:03.01 AS.seek 0ml
@02:09:43.83 AFV.select :carrierSolution
@02:09:43.95 delay 2 seconds
@02:09:45.97 AS.reconfigure ASconfig
@02:09:46.03 AS.seek 0.5ml
@02:10:15.07 delay 1 second
@02:10:16.09 AFV.select :holdingCoil
@02:10:16.20 ATV.dial :extSrc
@02:10:16.22 AS.reconfigure ASslow1
@02:10:16.28 AS.seek 0ml
@02:11:57.10 ATV.dial :air2
@02:11:58.50 AS.reconfigure ASslow0
@02:11:58.56 AS.seek 0.5ml
@02:12:49.19 ATV.dial :extSrc
@02:12:50.77 AS.seek 0.02ml
@02:13:39.38 delay 1 second
@02:13:40.41 ATV.dial :waste
@02:13:41.78 AS.reconfigure ASconfig
@02:13:41.83 AS.seek 0ml
@02:13:43.69 AFV.select :holdingCoil
@02:13:43.71 ATV.dial :waste
@02:13:43.74 AS.seek 0ml
@02:13:43.82 AFV.select :carrierSolution
@02:13:43.93 delay 2 seconds
@02:13:45.95 AS.seek 0.5ml
@02:14:14.99 delay 1 second
@02:14:16.01 AFV.select :holdingCoil
@02:14:16.12 ATV.dial :air3
@02:14:17.00 AS.reconfigure ASslow1
@02:14:17.06 AS.seek 0.52ml
@02:14:21.27 ATV.dial :air3
@02:14:21.36 AS.seek 0.53ml
@02:14:23.66 delay 2 seconds
@02:14:25.69 ATV.dial :bleach
@02:14:26.16 AS.seek 0.71ml
@02:15:02.63 delay 3 seconds
@02:15:05.66 ATV.dial :air3
@02:15:06.24 delay 3 seconds
@02:15:09.31 AS.seek 0.735ml
@02:15:14.70 delay 3 seconds
@02:15:17.73 ATV.dial :bleach
@02:15:18.38 AS.seek 0.72ml
@02:15:21.68 delay 3 seconds
@02:15:24.71 ATV.dial ASV
@02:15:25.83 ASV.dial :SPErecovery
@02:15:25.92 AS.seek 0.365ml
@02:16:37.57 ATV.dial :waste
@02:16:39.61 AS.reconfigure ASnorm
@02:16:39.67 AS.seek 0ml
@02:16:58.36 ATV.dial :air3
@02:16:59.24 AS.reconfigure ASslow1
@02:16:59.30 AS.seek 0.01ml
@02:17:01.74 delay 2 seconds
@02:17:03.77 ATV.dial :bleach
@02:17:04.25 AS.seek 0.58ml
@02:18:59.19 delay 3 seconds
@02:19:02.22 ATV.dial :air3
@02:19:02.80 delay 3 seconds
@02:19:05.88 AS.seek 0.605ml
@02:19:11.16 delay 3 seconds
@02:19:14.19 ATV.dial :bleach
@02:19:14.83 AS.seek 0.59ml
@02:19:18.10 delay 3 seconds
@02:19:21.13 ATV.dial :ASV
@02:19:22.24 ASV.dial :wasteR
@02:19:22.66 AS.seek 0ml
@02:21:21.68 delay 1:00
@02:22:21.72 ATV.dial ASV
@02:22:21.73 AFV.select :carrierSolution
@02:22:21.85 delay 2 seconds
@02:22:23.87 AS.reconfigure ASconfig
@02:22:23.92 AS.seek 1ml
@02:23:21.68 delay 1 second
@02:23:22.70 AFV.select :holdingCoil
@02:23:22.81 delay 1 second
@02:23:23.83 ASV.dial :wasteR
@02:23:23.84 AS.reconfigure ASslow1
@02:23:23.92 AS.seek 0ml
@02:26:45.30 delay 10 seconds
@02:26:55.34 ATV.dial :air3
@02:26:56.94 AS.seek 0.01ml
@02:26:59.24 delay 2 seconds
@02:27:01.27 ATV.dial :bleach
@02:27:01.91 AS.seek 0.58ml
@02:28:56.80 delay 3 seconds
@02:28:59.83 ATV.dial :air3
@02:29:00.41 delay 3 seconds
@02:29:03.48 AS.seek 0.605ml
@02:29:08.81 delay 3 seconds
@02:29:11.84 ATV.dial :bleach
@02:29:12.47 AS.seek 0.59ml
@02:29:15.77 delay 3 seconds
@02:29:18.80 ATV.dial ASV
@02:29:19.93 ASV.dial :SPEdelivery
@02:29:20.33 AS.seek 0ml
@02:31:19.22 delay 1:00
@02:32:19.25 AFV.select :carrierSolution
@02:32:19.36 delay 2 seconds
@02:32:21.38 AS.reconfigure ASconfig
@02:32:21.44 AS.seek 1ml
@02:33:19.21 delay 1 second
@02:33:20.23 AFV.select :holdingCoil
@02:33:20.34 delay 1 second
@02:33:21.36 ATV.dial ASV
@02:33:21.37 ASV.dial :SPEdelivery
@02:33:21.40 AS.reconfigure ASslow1
@02:33:21.46 AS.seek 0ml
@02:36:42.83 delay 10 seconds
@02:36:52.87 ATV.dial :air2
@02:36:53.77 AS.reconfigure ASslow0
@02:36:53.82 AS.seek 0.8ml
@02:38:14.61 delay 3 seconds
@02:38:17.64 ATV.dial :ASV
@02:38:18.71 ASV.dial :SPEdelivery
@02:38:18.73 AS.reconfigure ASslow1
@02:38:18.80 AS.seek 0.03ml
@02:40:53.93 delay 20 seconds
@02:41:13.97 ATV.dial :waste,via: :air3
@02:41:15.83 AS.reconfigure ASconfig
@02:41:15.88 AS.seek 0ml
@02:41:17.93 SPE.coast
@02:41:18.01 Removing Bleach from Mixing Coil
@02:41:18.02 AFV.select :carrierSolution
@02:41:18.14 delay 2 seconds
@02:41:20.16 AS.seek 0.5ml
@02:41:49.18 delay 1 second
@02:41:50.20 AFV.select :holdingCoil
@02:41:50.31 ATV.dial :ASV
@02:41:52.29 ASV.dial :SPErecovery
@02:41:53.38 AS.reconfigure ASslow1
@02:41:53.44 AS.seek 0.73ml
@02:42:40.02 ASV.dial :wasteD
@02:42:40.62 AS.reconfigure ASnorm
@02:42:40.68 AS.seek 0ml
@02:43:17.77 AFV.select :carrierSolution
@02:43:17.89 delay 2 seconds
@02:43:19.91 AS.reconfigure ASconfig
@02:43:19.97 AS.seek 0.5ml
@02:43:49.02 delay 1 second
@02:43:50.04 AFV.select :holdingCoil
@02:43:50.15 ATV.dial ASV
@02:43:50.16 ASV.dial :SPErecovery
@02:43:50.83 AS.reconfigure ASslow0
@02:43:50.89 AS.seek 0.3ml
@02:44:11.30 ATV.dial :air2
@02:44:12.24 AS.reconfigure ASslow1
@02:44:12.30 AS.seek 0.32ml
@02:44:16.69 ATV.dial ASV
@02:44:17.80 AS.reconfigure ASslow0
@02:44:17.86 AS.seek 0.57ml
@02:44:43.33 ASV.dial :wasteD
@02:44:43.94 AS.reconfigure ASnorm
@02:44:44.00 AS.seek 0ml
@02:45:13.07 AFV.select :carrierSolution
@02:45:13.19 delay 2 seconds
@02:45:15.21 AS.reconfigure ASconfig
@02:45:15.27 AS.seek 0.5ml
@02:45:44.36 delay 1 second
@02:45:45.38 AFV.select :holdingCoil
@02:45:45.49 ATV.dial ASV
@02:45:45.50 ASV.dial :SPErecovery
@02:45:46.17 AS.reconfigure ASslow0
@02:45:46.23 AS.seek 0.3ml
@02:46:06.65 ATV.dial :air2
@02:46:07.75 AS.reconfigure ASslow1
@02:46:07.81 AS.seek 0.32ml
@02:46:12.07 ATV.dial ASV
@02:46:13.19 AS.reconfigure ASslow0
@02:46:13.25 AS.seek 0.57ml
@02:46:38.66 ASV.dial :wasteD
@02:46:39.30 AS.reconfigure ASnorm
@02:46:39.35 AS.seek 0ml
@02:47:08.43 ATV.dial :air2
@02:47:09.48 AS.reconfigure ASslow0
@02:47:09.53 AS.seek 0.3ml
@02:47:40.07 ATV.dial :ASV
@02:47:41.12 ASV.dial :wasteD
@02:47:41.14 AS.reconfigure ASconfig
@02:47:41.20 AS.seek 0.05ml
@02:47:55.90 ATV.dial :waste
@02:47:57.91 AS.seek 0ml
@02:48:01.07 ASV.dial :SPEdelivery
@02:48:02.16 Cleaning PCR
@02:48:02.17 AFV.select :carrierSolution
@02:48:02.29 delay 2 seconds
@02:48:04.31 AS.seek 0.5ml
@02:48:33.33 delay 1 second
@02:48:34.35 AFV.select :holdingCoil
@02:48:34.46 ASV.dial :waste1
@02:48:34.83 ATV.dial :air3
@02:48:35.57 AS.reconfigure ASslow1
@02:48:35.63 AS.seek 0.51ml
@02:48:37.90 delay 2 seconds
@02:48:39.93 ATV.dial :bleach
@02:48:40.39 AS.seek 0.81ml
@02:49:40.97 delay 3 seconds
@02:49:44.00 ATV.dial :air3
@02:49:44.58 delay 3 seconds
@02:49:47.65 AS.seek 0.835ml
@02:49:52.95 delay 3 seconds
@02:49:55.98 ATV.dial :bleach
@02:49:56.61 AS.seek 0.82ml
@02:49:59.91 delay 3 seconds
@02:50:02.94 ARV.dial :waste
@02:50:03.57 delay 3 seconds
@02:50:06.60 ATV.dial :ARV
@02:50:07.52 AS.reconfigure ASconfig
@02:50:07.57 AS.seek 0.47ml
@02:50:27.99 AFV.select :carrierSolution
@02:50:28.11 delay 2 seconds
@02:50:30.13 AS.seek 1ml
@02:51:00.91 delay 1 second
@02:51:01.93 AFV.select :holdingCoil
@02:51:02.04 delay 1 second
@02:51:03.06 AS.reconfigure ASfast
@02:51:03.12 AS.seek 0ml
@02:51:30.46 AFV.select :carrierSolution
@02:51:30.58 delay 2 seconds
@02:51:32.60 AS.reconfigure ASconfig
@02:51:32.65 AS.seek 1ml
@02:52:30.51 delay 1 second
@02:52:31.53 AFV.select :holdingCoil
@02:52:31.64 delay 1 second
@02:52:32.66 AS.reconfigure ASfast
@02:52:32.72 AS.seek 0ml
@02:53:00.07 AFV.select :carrierSolution
@02:53:00.18 delay 2 seconds
@02:53:02.20 AS.reconfigure ASconfig
@02:53:02.25 AS.seek 1ml
@02:54:00.02 delay 1 second
@02:54:01.04 AFV.select :holdingCoil
@02:54:01.15 delay 1 second
@02:54:02.17 AS.reconfigure ASfast
@02:54:02.23 AS.seek 0ml
@02:54:29.57 ARV.dial between carrier and air1
@02:54:30.09 ATV.dial :air3
@02:54:31.88 AS.reconfigure ASslow1
@02:54:31.94 AS.seek 0.01ml
@02:54:34.38 delay 2 seconds
@02:54:36.41 ATV.dial :bleach
@02:54:37.05 AS.seek 0.81ml
@02:57:18.22 delay 3 seconds
@02:57:21.25 ATV.dial :air3
@02:57:21.83 delay 3 seconds
@02:57:24.91 AS.seek 0.835ml
@02:57:30.18 delay 3 seconds
@02:57:33.21 ATV.dial :bleach
@02:57:33.85 AS.seek 0.82ml
@02:57:37.19 delay 3 seconds
@02:57:40.22 ATV.dial PCR
@02:57:40.66 AS.reconfigure ASconfig
@02:57:40.71 AS.seek 0ml
@02:58:28.13 PCR.program :bleachProfile
@02:58:28.21 PCR program should complete in < 2:45
@02:58:28.22 PCR.run 10:00
@03:01:30.07 PCR.off
@03:01:30.11 AFV.select :carrierSolution
@03:01:30.22 delay 2 seconds
@03:01:32.24 AS.seek 1ml
@03:02:30.01 delay 1 second
@03:02:31.03 AFV.select :holdingCoil
@03:02:31.14 delay 1 second
@03:02:32.16 AS.reconfigure ASfast
@03:02:32.22 AS.seek 0ml
@03:02:59.55 delay 3 seconds
@03:03:02.57 AFV.select :carrierSolution
@03:03:02.68 delay 2 seconds
@03:03:04.70 AS.reconfigure ASconfig
@03:03:04.77 AS.seek 1ml
@03:04:02.54 delay 1 second
@03:04:03.56 AFV.select :holdingCoil
@03:04:03.67 delay 1 second
@03:04:04.69 AS.reconfigure ASfast
@03:04:04.75 AS.seek 0ml
@03:04:32.07 delay 3 seconds
@03:04:35.09 AFV.select :carrierSolution
@03:04:35.20 delay 2 seconds
@03:04:37.22 AS.reconfigure ASconfig
@03:04:37.28 AS.seek 1ml
@03:05:35.07 delay 1 second
@03:05:36.09 AFV.select :holdingCoil
@03:05:36.20 delay 1 second
@03:05:37.22 AS.reconfigure ASfast
@03:05:37.28 AS.seek 0ml
@03:06:04.63 delay 3 seconds
@03:06:07.65 AFV.select :carrierSolution
@03:06:07.76 delay 2 seconds
@03:06:09.78 AS.reconfigure ASconfig
@03:06:09.84 AS.seek 1ml
@03:07:07.60 delay 1 second
@03:07:08.62 AFV.select :holdingCoil
@03:07:08.73 delay 1 second
@03:07:09.75 AS.reconfigure ASfast
@03:07:09.81 AS.seek 0ml
@03:07:37.15 delay 3 seconds
@03:07:40.17 AFV.select :carrierSolution
@03:07:40.28 delay 2 seconds
@03:07:42.30 AS.reconfigure ASconfig
@03:07:42.36 AS.seek 1ml
@03:08:40.57 delay 1 second
@03:08:41.59 AFV.select :holdingCoil
@03:08:41.70 delay 1 second
@03:08:42.72 AS.reconfigure ASnorm
@03:08:42.78 AS.seek 0ml
@03:09:33.53 AFV.select :carrierSolution
@03:09:33.64 delay 2 seconds
@03:09:35.66 AS.reconfigure ASconfig
@03:09:35.72 AS.seek 1ml
@03:10:33.48 delay 1 second
@03:10:34.50 AFV.select :holdingCoil
@03:10:34.61 delay 1 second
@03:10:35.63 ATV.dial :waste
@03:10:36.94 AS.reconfigure ASnorm
@03:10:37.00 AS.seek 0.5ml
@03:11:02.57 ATV.dial :waste2
@03:11:03.95 AS.seek 0ml
@03:11:29.49 <mwj> Session Ended
@13:53:31.88CET23-Mar-21 <MAIN> ESP2 v3.30 I2C Gateway v3.06 for core bus
@13:53:33.97 
Configuration for mer 7/20/20 brent@mbari.org
/home/esp/esp2/type/shallow/mer/configure.rb
@13:53:34.05 
Configuration for MFB3 by preston@mbari.org
/home/esp/esp2/type/mfb/MFB3/configure.rb
@13:53:38.37 Polling Can every 10:00
@13:53:40.08 CS.configure :default
@13:53:40.12 CS.hold
@13:53:40.17 CS.configure :default
@13:53:40.25 PS.configure :default
@13:53:40.33 SS.configure :default
@13:53:40.36 SS.hold
@13:53:40.42 SS.configure :default
@13:53:40.50 CC.configure :default
@13:53:40.54 CC.hold
@13:53:40.61 CC.configure :default
@13:53:40.69 PC.configure :default
@13:53:40.73 PC.hold
@13:53:40.80 PC.configure :default
@13:53:40.90 Forearm.configure :loose
@13:53:40.94 Forearm.hold
@13:53:40.99 Forearm.configure :loose
@13:53:41.07 Elbow.configure :loose
@13:53:41.11 Elbow.hold
@13:53:41.16 Elbow.configure :loose
@13:53:41.26 SC.configure :loose
@13:53:41.30 SC.hold
@13:53:41.36 SC.configure :loose
@13:53:41.44 SE.configure :default
@13:53:41.48 SE.hold
@13:53:41.53 SE.configure :default
@13:53:41.57 CH.coast
@13:53:41.61 CH.configure :default
@13:53:41.64 PH.coast
@13:53:41.69 PH.configure :default
@13:53:41.79 SPE.coast
@13:53:41.83 SPE.configure SPEconfig
@13:53:41.90 AS.configure ASconfig
@13:53:41.94 AS.hold
@13:53:42.00 AS.configure ASconfig
@13:53:42.05 PCR.off
@13:53:42.08 PCR.pidtemp= 50,0.1,0,300,25,25
@13:53:42.11 PCR.caltemp= 2.5051,0.0372,0
@13:53:42.14 All dwarf controllers running firmware version 3.75
@13:53:42.31 Cleanly exited at 13:53:10CET23-Mar-21
@13:53:42.34 Loading /home/esp/esp2/mission/GENOMAP.rb mission
@13:53:42.48 <MFBPCR> Extracting Nucleic Acid from extSrc
@13:53:42.51 SPE.hold 2:46:40,55C
@13:53:42.55 ASV.dial :SPEdelivery
@13:53:42.98 delay 20 seconds
@13:53:44.46 <mwj> Session Begun
@13:54:03.02 <MFBPCR> AFV.select :holdingCoil
@13:54:03.14 ATV.dial :air2
@13:54:04.42 AS.reconfigure ASslow1
@13:54:04.48 AS.seek 0.07ml
@13:54:18.87 delay 3 seconds
@13:54:21.90 ATV.dial :extSrc
@13:54:23.40 AS.seek 0.47ml
@13:55:44.15 delay 5 seconds
@13:55:49.23 Solid Phase Extraction Heater at 39.1C
@13:55:49.24 ATV.dial ASV
@13:55:50.11 ASV.dial :SPEdelivery
@13:55:50.20 AS.seek 0.042ml
@13:57:16.57 delay 10 seconds
@13:57:26.61 Rinsing external lysate line extSrc
@13:57:26.62 AFV.select :carrierSolution
@13:57:26.74 delay 2 seconds
@13:57:28.76 AS.reconfigure ASconfig
@13:57:28.82 AS.seek 1ml
@13:58:24.20 delay 1 second
@13:58:25.22 AFV.select :holdingCoil
@13:58:25.33 delay 1 second
@13:58:26.35 ATV.dial :extSrc
@13:58:27.18 AS.reconfigure ASnorm
@13:58:27.23 AS.seek 0ml
@13:59:17.93 ATV.dial :air2
@13:59:19.53 AS.reconfigure ASslow0
@13:59:19.59 AS.seek 0.05ml
@13:59:25.00 ATV.dial :extSrc
@13:59:26.60 AS.seek 0.01ml
@13:59:30.97 ATV.dial :waste
@13:59:32.35 AS.reconfigure ASconfig
@13:59:32.40 AS.seek 0ml
@13:59:33.63 ethanol wash
@13:59:33.64 ATV.dial :air2
@13:59:34.82 AS.reconfigure ASslow2
@13:59:34.88 AS.seek 0.01ml
@13:59:45.32 delay 3 seconds
@13:59:48.35 Pulling 0.4ml of Ethanol Wash
@13:59:48.36 ATV.dial :ethanol
@13:59:49.24 AS.reconfigure ASslow1
@13:59:49.30 AS.seek 0.41ml
@14:01:09.98 delay 3 seconds
@14:01:13.01 ATV.dial :air1
@14:01:13.43 delay 3 seconds
@14:01:16.50 AS.seek 0.435ml
@14:01:21.82 delay 3 seconds
@14:01:24.85 ATV.dial :ethanol
@14:01:25.48 AS.seek 0.42ml
@14:01:28.87 delay 3 seconds
@14:01:31.90 ATV.dial ASV
@14:01:33.31 AS.seek 0.093ml
@14:02:39.31 delay 3 seconds
@14:02:42.34 ATV.dial :waste2
@14:02:43.15 AS.reconfigure ASconfig
@14:02:43.20 AS.seek 0ml
@14:02:48.83 ATV.dial :air2
@14:02:49.26 AS.reconfigure ASslow1
@14:02:49.31 AS.seek 1ml
@14:06:10.76 delay 5 seconds
@14:06:15.79 ATV.dial ASV
@14:06:16.83 ASV.dial :SPEdelivery
@14:06:16.86 AS.seek 0.02ml
@14:09:34.25 delay 5 seconds
@14:09:39.28 ATV.dial :air2
@14:09:40.37 AS.seek 0.52ml
@14:11:21.22 delay 5 seconds
@14:11:26.25 ATV.dial ASV
@14:11:27.29 ASV.dial :wasteR
@14:11:27.68 AS.seek 0.3ml
@14:12:12.22 delay 20 seconds
@14:12:32.26 AS.seek 0.07ml
@14:13:18.78 delay 20 seconds
@14:13:38.81 ATV.dial :waste2
@14:13:39.63 AS.reconfigure ASconfig
@14:13:39.68 AS.seek 0ml
@14:13:44.01 Elution
@14:13:44.02 ATV.dial :air1
@14:13:45.20 AS.reconfigure ASslow1
@14:13:45.25 AS.seek 0.05ml
@14:13:55.61 delay 3 seconds
@14:13:58.64 ATV.dial :ElutionWater
@14:13:59.35 AS.seek 0.11ml
@14:14:11.68 delay 3 seconds
@14:14:14.71 ATV.dial ASV,via: :air2
@14:14:16.96 ASV.dial :SPEelution
@14:14:17.58 AFV.select :carrierSolution
@14:14:17.74 AS.reconfigure ASnorm
@14:14:17.80 AS.seek 0.76ml
@14:14:50.90 delay 3 seconds
@14:14:53.92 AFV.select :holdingCoil
@14:14:54.07 AS.reconfigure ASslow1
@14:14:54.13 AS.seek 0.475ml
@14:15:51.73 delay 5 seconds
@14:15:56.81 AS.reconfigure ASslow2
@14:15:56.87 AS.seek 0.44ml
@14:16:32.20 delay 1:00
@14:17:32.24 pushing eluate to holding coil
@14:17:32.31 AS.seek 0.28ml
@14:18:14.52 <mwj2> Session Begun
@14:19:33.78 -> showlog 10
@14:20:13.62 <MFBPCR> delay 30 seconds
@14:20:43.66 ATV.dial :waste,via: :air3
@14:20:45.52 AS.reconfigure ASconfig
@14:20:45.59 AS.seek 0ml
@14:21:02.00 Parking Bleach in LysateSource line and SPEcolumn
@14:21:02.01 ATV.dial :air3
@14:21:02.93 AS.reconfigure ASslow1
@14:21:03.00 AS.seek 0.03ml
@14:21:09.37 ATV.dial :ElutionWater
@14:21:10.03 AS.seek 0.06ml
@14:21:16.34 ATV.dial :air3
@14:21:17.01 AS.seek 0.07ml
@14:21:19.37 delay 2 seconds
@14:21:21.40 ATV.dial :bleach
@14:21:21.90 AS.seek 0.15ml
@14:21:38.28 delay 3 seconds
@14:21:41.31 ATV.dial :air3
@14:21:41.92 delay 3 seconds
@14:21:44.99 AS.seek 0.175ml
@14:21:50.33 delay 3 seconds
@14:21:53.36 ATV.dial :bleach
@14:21:54.01 AS.seek 0.16ml
@14:21:57.22 delay 3 seconds
@14:22:00.25 ATV.dial :extSrc
@14:22:00.91 AS.reconfigure ASconfig
@14:22:00.97 AS.seek 0.015ml
@14:22:09.62 ATV.dial :waste
@14:22:11.18 AS.seek 0ml
@14:22:12.36 ATV.dial :air3
@14:22:13.28 AS.reconfigure ASslow1
@14:22:13.35 AS.seek 0.01ml
@14:22:15.72 delay 2 seconds
@14:22:17.75 ATV.dial :bleach
@14:22:18.24 AS.seek 0.58ml
@14:24:13.14 delay 3 seconds
@14:24:16.17 ATV.dial :air3
@14:24:16.78 delay 3 seconds
@14:24:19.85 AS.seek 0.605ml
@14:24:25.15 delay 3 seconds
@14:24:28.18 ATV.dial :bleach
@14:24:28.82 AS.seek 0.59ml
@14:24:32.17 delay 3 seconds
@14:24:35.20 ATV.dial :ASV
@14:24:36.31 ASV.dial :wasteR
@14:24:37.02 AS.seek 0ml
@14:26:35.97 delay 1:00
@14:27:36.00 AFV.select :carrierSolution
@14:27:36.11 delay 2 seconds
@14:27:38.13 AS.reconfigure ASconfig
@14:27:38.19 AS.seek 1ml
@14:28:35.93 delay 1 second
@14:28:36.95 AFV.select :holdingCoil
@14:28:37.06 delay 1 second
@14:28:38.08 ATV.dial ASV
@14:28:38.09 ASV.dial :wasteR
@14:28:38.13 AS.reconfigure ASslow1
@14:28:38.20 AS.seek 0ml
@14:31:59.72 delay 20 seconds
@14:32:19.76 ATV.dial :air3
@14:32:21.39 AS.seek 0.59ml
@14:34:20.23 delay 3 seconds
@14:34:23.26 ATV.dial :ASV
@14:34:24.78 ASV.dial :wasteR
@14:34:24.88 AS.seek 0.09ml
@14:36:05.72 delay 30 seconds
@14:36:35.76 ATV.dial :extSrc
@14:36:36.59 delay 3 seconds
@14:36:39.62 ATV.dial :ElutionWater
@14:36:40.83 AS.seek 0.12ml
@14:36:47.15 ATV.dial :air3
@14:36:47.82 AS.seek 0.13ml
@14:36:50.17 delay 2 seconds
@14:36:52.20 ATV.dial :bleach
@14:36:52.70 AS.seek 0.23ml
@14:37:13.11 delay 3 seconds
@14:37:16.14 ATV.dial :air3
@14:37:16.75 delay 3 seconds
@14:37:19.82 AS.seek 0.255ml
@14:37:25.12 delay 3 seconds
@14:37:28.15 ATV.dial :bleach
@14:37:29.25 AS.seek 0.24ml
@14:37:32.56 delay 3 seconds
@14:37:35.59 ATV.dial :ASV
@14:37:36.72 ASV.dial :SPEdelivery
@14:37:37.11 AFV.select :carrierSolution
@14:37:37.24 delay 2 seconds
@14:37:39.26 AS.reconfigure ASconfig
@14:37:39.32 AS.seek 0.5ml
@14:37:54.58 delay 1 second
@14:37:55.60 AFV.select :holdingCoil
@14:37:55.75 AS.reconfigure ASslow1
@14:37:55.82 AS.seek 0.17ml
@14:39:02.48 SPE.hold 2:46:40,95C
@14:39:02.55 delay 3:00
@14:42:02.58 AFV.select :carrierSolution
@14:42:02.71 delay 2 seconds
@14:42:04.73 AS.reconfigure ASconfig
@14:42:04.79 AS.seek 1ml
@14:42:52.81 delay 1 second
@14:42:53.83 AFV.select :holdingCoil
@14:42:53.94 delay 1 second
@14:42:54.96 ASV.dial :wasteD
@14:42:56.16 AS.seek 0ml
@14:43:53.95 Priming Eluate
@14:43:53.96 AFV.select :carrierSolution
@14:43:54.11 delay 2 seconds
@14:43:56.13 AS.seek 0.5ml
@14:44:25.15 delay 1 second
@14:44:26.17 AFV.select :holdingCoil
@14:44:26.28 ATV.dial ASV
@14:44:26.29 ASV.dial :SPErecovery
@14:44:26.93 Swish #1 of 2
@14:44:27.03 AS.reconfigure ASslow0
@14:44:27.09 AS.seek 0.6ml
@14:44:37.54 AS.seek 0.5ml
@14:44:47.97 Swish #2 of 2
@14:44:48.07 AS.seek 0.6ml
@14:44:58.56 AS.seek 0.5ml
@14:45:08.98 delay 3 seconds
@14:45:12.05 AS.reconfigure ASslow1
@14:45:12.12 AS.seek 0.684ml
@14:45:49.42 delay 3 seconds
@14:45:52.45 Eluate primed
@14:45:52.46 ATV.dial :waste2
@14:45:53.15 AS.reconfigure ASnorm
@14:45:53.22 AS.seek 0ml
@14:46:28.04 SPE.coast
@14:46:28.13 ASV.dial :SPErecovery
@14:46:28.15 Setting up PCR for ACYPP2 -- writing /var/log/esp/ACYPP221mar2314h.pcr
@14:46:28.18 Building PCR Slug from ASV with MM1 mix, PP2 primer
@14:46:28.20 Priming with carrier
@14:46:28.24 ATV.dial ARV
@14:46:29.29 ARV.dial :carrier
@14:46:29.66 AS.seek 0.5ml
@14:46:55.20 AFV.select :carrierSolution
@14:46:55.34 delay 2 seconds
@14:46:57.36 AS.reconfigure ASconfig
@14:46:57.44 AS.seek 1ml
@14:47:26.47 delay 1 second
@14:47:27.49 AFV.select :holdingCoil
@14:47:27.60 delay 1 second
@14:47:28.62 ATV.dial :waste
@14:47:30.24 AS.reconfigure ASnorm
@14:47:30.31 AS.seek 0ml
@14:48:21.03 AFV.select :carrierSolution
@14:48:21.17 delay 2 seconds
@14:48:23.19 AS.reconfigure ASconfig
@14:48:23.26 AS.seek 0.5ml
@14:48:52.29 delay 1 second
@14:48:53.31 AFV.select :holdingCoil
@14:48:53.44 Building Slug
@14:48:53.45 ATV.dial :ARV
@14:48:55.24 delay 2 seconds
@14:48:57.26 ARV.dial :air1
@14:48:57.67 delay 2 seconds
@14:48:59.74 AS.reconfigure ASslow2
@14:48:59.82 AS.seek 0.506ml
@14:49:06.09 delay 2 seconds
@14:49:08.12 ARV.dial :MM1
@14:49:08.75 delay 2 seconds
@14:49:10.78 Adding MM1
@14:49:10.86 AS.seek 0.524ml
@14:49:29.53 delay 25 seconds
@14:49:54.56 ARV.dial :PP2
@14:49:54.98 Adding PP2
@14:49:54.99 delay 2 seconds
@14:49:57.09 AS.seek 0.53ml
@14:50:03.47 delay 10 seconds
@14:50:13.51 ARV.dial :air2
@14:50:13.93 adding ARV air
@14:50:13.94 delay 2 seconds
@14:50:16.04 AS.seek 0.536ml
@14:50:22.22 delay 5 seconds
@14:50:27.25 ARV.dial :carrier
@14:50:28.61 delay 7 seconds
@14:50:35.70 AS.seek 0.594ml
@14:51:34.18 delay 5 seconds
@14:51:39.21 ATV.dial :ASV
@14:51:39.64 delay 2 seconds
@14:51:41.72 AS.seek 0.6ml
@14:51:48.09 delay 4 seconds
@14:51:52.12 ATV.dial ARV
@14:51:52.70 delay 2 seconds
@14:51:54.73 ARV.dial :carrier
@14:51:54.74 delay 2 seconds
@14:51:56.82 AS.seek 0.65ml
@14:52:47.31 delay 4 seconds
@14:52:51.34 ATV.dial PCR
@14:52:51.98 ASV.dial :waste1
@14:52:52.80 ARV.dial :air1
@14:52:53.24 Positioning Slug by 0.324ml
@14:52:53.33 AS.seek 0.326ml
@14:58:19.52 delay 5 seconds
@14:58:24.55 ATV.dial ARV
@14:58:25.36 ASV.dial :PCRlock
@14:58:25.75 delay 5 seconds
@14:58:30.78 Running ACYPP2 Profile60CIQ2 thermocycling
@14:58:30.80 <PCR> PCR.program Profile60CIQ2
@14:58:30.93 Profile60CIQ2 should complete in < 1:47:50
@14:58:30.94 PCR.run 2:19:47.5
@15:44:48.02 <mwj2> Session Ended
@16:26:57.26 <PCR> PCR.off
@16:26:58.25 <MFBPCR> Running ACYPP2 MeltProfile60 thermocycling
@16:26:58.26 <PCR> PCR.program MeltProfile60
@16:26:58.36 MeltProfile60 should complete in < 11:55
@16:26:58.37 PCR.run 19:53.75
@16:34:23.80 PCR.off
@16:34:24.73 <MFBPCR> PCR for ACYPP2 completed -- closed /var/log/esp/ACYPP221mar2314h.pcr
@16:34:24.80 Cleaning PCR
@16:34:24.81 AFV.select :carrierSolution
@16:34:24.93 delay 2 seconds
@16:34:26.95 AS.reconfigure ASconfig
@16:34:27.00 AS.seek 0.5ml
@16:34:37.26 delay 1 second
@16:34:38.28 AFV.select :holdingCoil
@16:34:38.39 ASV.dial :waste1
@16:34:38.78 ATV.dial :air3
@16:34:40.15 AS.reconfigure ASslow1
@16:34:40.21 AS.seek 0.51ml
@16:34:42.49 delay 2 seconds
@16:34:44.52 ATV.dial :bleach
@16:34:45.17 AS.seek 0.81ml
@16:35:45.75 delay 3 seconds
@16:35:48.78 ATV.dial :air3
@16:35:49.36 delay 3 seconds
@16:35:52.43 AS.seek 0.835ml
@16:35:57.76 delay 3 seconds
@16:36:00.79 ATV.dial :bleach
@16:36:01.42 AS.seek 0.82ml
@16:36:04.76 delay 3 seconds
@16:36:07.79 ARV.dial :waste
@16:36:08.42 delay 3 seconds
@16:36:11.45 ATV.dial :ARV
@16:36:12.39 AS.reconfigure ASconfig
@16:36:12.44 AS.seek 0.47ml
@16:36:32.86 AFV.select :carrierSolution
@16:36:32.98 delay 2 seconds
@16:36:35.00 AS.seek 1ml
@16:37:05.75 delay 1 second
@16:37:06.77 AFV.select :holdingCoil
@16:37:06.88 delay 1 second
@16:37:07.90 AS.reconfigure ASfast
@16:37:07.95 AS.seek 0ml
@16:37:35.29 AFV.select :carrierSolution
@16:37:35.41 delay 2 seconds
@16:37:37.43 AS.reconfigure ASconfig
@16:37:37.48 AS.seek 1ml
@16:38:35.23 delay 1 second
@16:38:36.25 AFV.select :holdingCoil
@16:38:36.36 delay 1 second
@16:38:37.38 AS.reconfigure ASfast
@16:38:37.44 AS.seek 0ml
@16:39:04.79 AFV.select :carrierSolution
@16:39:04.90 delay 2 seconds
@16:39:06.92 AS.reconfigure ASconfig
@16:39:06.97 AS.seek 1ml
@16:40:04.73 delay 1 second
@16:40:05.75 AFV.select :holdingCoil
@16:40:05.86 delay 1 second
@16:40:06.88 AS.reconfigure ASfast
@16:40:06.93 AS.seek 0ml
@16:40:34.26 ARV.dial between carrier and air1
@16:40:34.79 ATV.dial :air3
@16:40:36.15 AS.reconfigure ASslow1
@16:40:36.21 AS.seek 0.01ml
@16:40:38.54 delay 2 seconds
@16:40:40.57 ATV.dial :bleach
@16:40:41.21 AS.seek 0.81ml
@16:43:22.33 delay 3 seconds
@16:43:25.36 ATV.dial :air3
@16:43:25.94 delay 3 seconds
@16:43:29.01 AS.seek 0.835ml
@16:43:34.31 delay 3 seconds
@16:43:37.34 ATV.dial :bleach
@16:43:37.98 AS.seek 0.82ml
@16:43:41.23 delay 3 seconds
@16:43:44.26 ATV.dial PCR
@16:43:44.68 AS.reconfigure ASconfig
@16:43:44.73 AS.seek 0ml
@16:44:32.15 PCR.program :bleachProfile
@16:44:32.22 PCR program should complete in < 2:45
@16:44:32.24 PCR.run 10:00
@16:47:28.45 PCR.off
@16:47:28.50 AFV.select :carrierSolution
@16:47:28.61 delay 2 seconds
@16:47:30.63 AS.seek 1ml
@16:48:28.42 delay 1 second
@16:48:29.44 AFV.select :holdingCoil
@16:48:29.55 delay 1 second
@16:48:30.57 AS.reconfigure ASfast
@16:48:30.63 AS.seek 0ml
@16:48:57.98 delay 3 seconds
@16:49:01.00 AFV.select :carrierSolution
@16:49:01.11 delay 2 seconds
@16:49:03.13 AS.reconfigure ASconfig
@16:49:03.18 AS.seek 1ml
@16:50:00.94 delay 1 second
@16:50:01.96 AFV.select :holdingCoil
@16:50:02.07 delay 1 second
@16:50:03.09 AS.reconfigure ASfast
@16:50:03.15 AS.seek 0ml
@16:50:30.48 delay 3 seconds
@16:50:33.50 AFV.select :carrierSolution
@16:50:33.61 delay 2 seconds
@16:50:35.63 AS.reconfigure ASconfig
@16:50:35.70 AS.seek 1ml
@16:51:33.45 delay 1 second
@16:51:34.47 AFV.select :holdingCoil
@16:51:34.58 delay 1 second
@16:51:35.60 AS.reconfigure ASfast
@16:51:35.66 AS.seek 0ml
@16:52:03.00 delay 3 seconds
@16:52:06.02 AFV.select :carrierSolution
@16:52:06.13 delay 2 seconds
@16:52:08.15 AS.reconfigure ASconfig
@16:52:08.20 AS.seek 1ml
@16:53:06.01 delay 1 second
@16:53:07.03 AFV.select :holdingCoil
@16:53:07.14 delay 1 second
@16:53:08.16 AS.reconfigure ASfast
@16:53:08.22 AS.seek 0ml
@16:53:35.67 delay 3 seconds
@16:53:38.69 AFV.select :carrierSolution
@16:53:38.80 delay 2 seconds
@16:53:40.82 AS.reconfigure ASconfig
@16:53:40.88 AS.seek 1ml
@16:54:38.62 delay 1 second
@16:54:39.64 AFV.select :holdingCoil
@16:54:39.75 delay 1 second
@16:54:40.77 AS.reconfigure ASnorm
@16:54:40.82 AS.seek 0ml
@16:55:31.59 Setting up PCR for AOPP1 -- writing /var/log/esp/AOPP121mar2316h.pcr
@16:55:31.61 Building PCR Slug from ASV with MM1 mix, PP1 primer
@16:55:31.63 Priming with carrier
@16:55:31.65 ATV.dial ARV
@16:55:32.29 ARV.dial :carrier
@16:55:32.64 AS.seek 0.5ml
@16:55:58.22 AFV.select :carrierSolution
@16:55:58.34 delay 2 seconds
@16:56:00.36 AS.reconfigure ASconfig
@16:56:00.42 AS.seek 1ml
@16:56:29.44 delay 1 second
@16:56:30.46 AFV.select :holdingCoil
@16:56:30.57 delay 1 second
@16:56:31.59 ATV.dial :waste
@16:56:33.38 AS.reconfigure ASnorm
@16:56:33.44 AS.seek 0ml
@16:57:24.12 AFV.select :carrierSolution
@16:57:24.24 delay 2 seconds
@16:57:26.26 AS.reconfigure ASconfig
@16:57:26.32 AS.seek 0.5ml
@16:57:55.39 delay 1 second
@16:57:56.41 AFV.select :holdingCoil
@16:57:56.52 Building Slug
@16:57:56.53 ATV.dial :ARV
@16:57:58.29 delay 2 seconds
@16:58:00.32 ARV.dial :air1
@16:58:00.73 delay 2 seconds
@16:58:02.80 AS.reconfigure ASslow2
@16:58:02.86 AS.seek 0.506ml
@16:58:09.14 delay 2 seconds
@16:58:11.17 ARV.dial :MM1
@16:58:11.79 delay 2 seconds
@16:58:13.82 Adding MM1
@16:58:13.89 AS.seek 0.524ml
@16:58:32.22 delay 25 seconds
@16:58:57.25 ARV.dial :PP1
@16:58:57.65 Adding PP1
@16:58:57.66 delay 2 seconds
@16:58:59.74 AS.seek 0.53ml
@16:59:06.26 delay 10 seconds
@16:59:16.30 ARV.dial :air1
@16:59:16.71 adding ARV air
@16:59:16.72 delay 2 seconds
@16:59:18.79 AS.seek 0.536ml
@16:59:25.17 delay 5 seconds
@16:59:30.20 ARV.dial :carrier
@16:59:30.62 delay 7 seconds
@16:59:37.71 AS.seek 0.594ml
@17:00:36.59 delay 5 seconds
@17:00:41.62 ATV.dial :ASV
@17:00:42.03 delay 2 seconds
@17:00:44.11 AS.seek 0.6ml
@17:00:50.45 delay 4 seconds
@17:00:54.48 ATV.dial ARV
@17:00:55.06 delay 2 seconds
@17:00:57.09 ARV.dial :carrier
@17:00:57.10 delay 2 seconds
@17:00:59.18 AS.seek 0.65ml
@17:01:49.90 delay 4 seconds
@17:01:53.93 ATV.dial PCR
@17:01:54.58 ASV.dial :waste1
@17:01:54.60 ARV.dial :air1
@17:01:55.01 Positioning Slug by 0.324ml
@17:01:55.10 AS.seek 0.326ml
@17:07:21.28 delay 5 seconds
@17:07:26.31 ATV.dial ARV
@17:07:27.12 ASV.dial :PCRlock
@17:07:27.95 delay 5 seconds
@17:07:32.98 Running AOPP1 Profile60CIQ1 thermocycling
@17:07:32.99 <PCR> PCR.program Profile60CIQ1
@17:07:33.12 Profile60CIQ1 should complete in < 1:56:15
@17:07:33.13 PCR.run 2:30:18.75
@18:46:13.68 PCR.off
@18:46:14.24 <MFBPCR> Running AOPP1 MeltProfile60 thermocycling
@18:46:14.25 <PCR> PCR.program MeltProfile60
@18:46:14.36 MeltProfile60 should complete in < 11:55
@18:46:14.37 PCR.run 19:53.75
@18:53:42.98 PCR.off
@18:53:44.13 <MFBPCR> PCR for AOPP1 completed -- closed /var/log/esp/AOPP121mar2316h.pcr
@18:53:44.20 Cleaning PCR
@18:53:44.21 AFV.select :carrierSolution
@18:53:44.34 delay 2 seconds
@18:53:46.36 AS.reconfigure ASconfig
@18:53:46.42 AS.seek 0.5ml
@18:53:56.70 delay 1 second
@18:53:57.72 AFV.select :holdingCoil
@18:53:57.83 ASV.dial :waste1
@18:53:58.22 ATV.dial :air3
@18:53:59.59 AS.reconfigure ASslow1
@18:53:59.65 AS.seek 0.51ml
@18:54:02.04 delay 2 seconds
@18:54:04.06 ATV.dial :bleach
@18:54:04.72 AS.seek 0.81ml
@18:55:05.34 delay 3 seconds
@18:55:08.37 ATV.dial :air3
@18:55:08.97 delay 3 seconds
@18:55:12.04 AS.seek 0.835ml
@18:55:17.36 delay 3 seconds
@18:55:20.39 ATV.dial :bleach
@18:55:21.03 AS.seek 0.82ml
@18:55:24.37 delay 3 seconds
@18:55:27.40 ARV.dial :waste
@18:55:28.03 delay 3 seconds
@18:55:31.06 ATV.dial :ARV
@18:55:32.00 AS.reconfigure ASconfig
@18:55:32.05 AS.seek 0.47ml
@18:55:52.47 AFV.select :carrierSolution
@18:55:52.59 delay 2 seconds
@18:55:54.61 AS.seek 1ml
@18:56:25.39 delay 1 second
@18:56:26.41 AFV.select :holdingCoil
@18:56:26.52 delay 1 second
@18:56:27.54 AS.reconfigure ASfast
@18:56:27.60 AS.seek 0ml
@18:56:54.94 AFV.select :carrierSolution
@18:56:55.06 delay 2 seconds
@18:56:57.08 AS.reconfigure ASconfig
@18:56:57.13 AS.seek 1ml
@18:57:54.87 delay 1 second
@18:57:55.89 AFV.select :holdingCoil
@18:57:56.00 delay 1 second
@18:57:57.02 AS.reconfigure ASfast
@18:57:57.08 AS.seek 0ml
@18:58:24.41 AFV.select :carrierSolution
@18:58:24.53 delay 2 seconds
@18:58:26.55 AS.reconfigure ASconfig
@18:58:26.60 AS.seek 1ml
@18:59:24.36 delay 1 second
@18:59:25.38 AFV.select :holdingCoil
@18:59:25.49 delay 1 second
@18:59:26.51 AS.reconfigure ASfast
@18:59:26.56 AS.seek 0ml
@18:59:53.91 ARV.dial between carrier and air1
@18:59:54.45 ATV.dial :air3
@18:59:55.81 AS.reconfigure ASslow1
@18:59:55.87 AS.seek 0.01ml
@18:59:58.28 delay 2 seconds
@19:00:00.31 ATV.dial :bleach
@19:00:00.95 AS.seek 0.81ml
@19:02:42.08 delay 3 seconds
@19:02:45.11 ATV.dial :air3
@19:02:45.69 delay 3 seconds
@19:02:48.76 AS.seek 0.835ml
@19:02:54.04 delay 3 seconds
@19:02:57.07 ATV.dial :bleach
@19:02:57.70 AS.seek 0.82ml
@19:03:01.00 delay 3 seconds
@19:03:04.03 ATV.dial PCR
@19:03:04.45 AS.reconfigure ASconfig
@19:03:04.50 AS.seek 0ml
@19:03:51.92 PCR.program :bleachProfile
@19:03:51.99 PCR program should complete in < 2:45
@19:03:52.01 PCR.run 10:00
@19:06:48.20 PCR.off
@19:06:48.24 AFV.select :carrierSolution
@19:06:48.35 delay 2 seconds
@19:06:50.37 AS.seek 1ml
@19:07:48.11 delay 1 second
@19:07:49.13 AFV.select :holdingCoil
@19:07:49.24 delay 1 second
@19:07:50.26 AS.reconfigure ASfast
@19:07:50.32 AS.seek 0ml
@19:08:17.67 delay 3 seconds
@19:08:20.69 AFV.select :carrierSolution
@19:08:20.80 delay 2 seconds
@19:08:22.82 AS.reconfigure ASconfig
@19:08:22.88 AS.seek 1ml
@19:09:20.64 delay 1 second
@19:09:21.66 AFV.select :holdingCoil
@19:09:21.77 delay 1 second
@19:09:22.79 AS.reconfigure ASfast
@19:09:22.85 AS.seek 0ml
@19:09:50.17 delay 3 seconds
@19:09:53.19 AFV.select :carrierSolution
@19:09:53.30 delay 2 seconds
@19:09:55.32 AS.reconfigure ASconfig
@19:09:55.39 AS.seek 1ml
@19:10:53.19 delay 1 second
@19:10:54.21 AFV.select :holdingCoil
@19:10:54.32 delay 1 second
@19:10:55.34 AS.reconfigure ASfast
@19:10:55.40 AS.seek 0ml
@19:11:22.75 delay 3 seconds
@19:11:25.77 AFV.select :carrierSolution
@19:11:25.88 delay 2 seconds
@19:11:27.90 AS.reconfigure ASconfig
@19:11:27.96 AS.seek 1ml
@19:12:25.69 delay 1 second
@19:12:26.71 AFV.select :holdingCoil
@19:12:26.82 delay 1 second
@19:12:27.84 AS.reconfigure ASfast
@19:12:27.90 AS.seek 0ml
@19:12:55.22 delay 3 seconds
@19:12:58.24 AFV.select :carrierSolution
@19:12:58.35 delay 2 seconds
@19:13:00.37 AS.reconfigure ASconfig
@19:13:00.44 AS.seek 1ml
@19:13:58.22 delay 1 second
@19:13:59.24 AFV.select :holdingCoil
@19:13:59.35 delay 1 second
@19:14:00.37 AS.reconfigure ASnorm
@19:14:00.42 AS.seek 0ml
@19:14:51.17 Setting up PCR for AARPP4 -- writing /var/log/esp/AARPP421mar2319h.pcr
@19:14:51.18 Building PCR Slug from ASV with MM2 mix, PP4 primer
@19:14:51.21 Priming with carrier
@19:14:51.22 ATV.dial ARV
@19:14:51.89 ARV.dial :carrier
@19:14:52.25 AS.seek 0.5ml
@19:15:17.80 AFV.select :carrierSolution
@19:15:17.92 delay 2 seconds
@19:15:19.94 AS.reconfigure ASconfig
@19:15:20.01 AS.seek 1ml
@19:15:49.03 delay 1 second
@19:15:50.05 AFV.select :holdingCoil
@19:15:50.16 delay 1 second
@19:15:51.18 ATV.dial :waste
@19:15:52.96 AS.reconfigure ASnorm
@19:15:53.02 AS.seek 0ml
@19:16:43.72 AFV.select :carrierSolution
@19:16:43.84 delay 2 seconds
@19:16:45.86 AS.reconfigure ASconfig
@19:16:45.92 AS.seek 0.5ml
@19:17:14.97 delay 1 second
@19:17:15.99 AFV.select :holdingCoil
@19:17:16.10 Building Slug
@19:17:16.11 ATV.dial :ARV
@19:17:17.88 delay 2 seconds
@19:17:19.91 ARV.dial :air1
@19:17:20.31 delay 2 seconds
@19:17:22.38 AS.reconfigure ASslow2
@19:17:22.44 AS.seek 0.506ml
@19:17:28.73 delay 2 seconds
@19:17:30.76 ARV.dial :MM2
@19:17:32.33 delay 2 seconds
@19:17:34.36 Adding MM2
@19:17:34.43 AS.seek 0.524ml
@19:17:52.63 delay 25 seconds
@19:18:17.66 ARV.dial :PP4
@19:18:18.07 Adding PP4
@19:18:18.08 delay 2 seconds
@19:18:20.15 AS.seek 0.53ml
@19:18:26.34 delay 10 seconds
@19:18:36.38 ARV.dial :air3
@19:18:36.79 adding ARV air
@19:18:36.80 delay 2 seconds
@19:18:38.87 AS.seek 0.536ml
@19:18:45.06 delay 5 seconds
@19:18:50.09 ARV.dial :carrier
@19:18:51.39 delay 7 seconds
@19:18:58.46 AS.seek 0.594ml
@19:19:56.94 delay 5 seconds
@19:20:01.97 ATV.dial :ASV
@19:20:02.39 delay 2 seconds
@19:20:04.46 AS.seek 0.6ml
@19:20:11.03 delay 4 seconds
@19:20:15.06 ATV.dial ARV
@19:20:15.64 delay 2 seconds
@19:20:17.67 ARV.dial :carrier
@19:20:18.09 delay 2 seconds
@19:20:20.17 AS.seek 0.65ml
@19:21:11.06 delay 4 seconds
@19:21:15.09 ATV.dial PCR
@19:21:15.74 ASV.dial :waste1
@19:21:15.76 ARV.dial :air1
@19:21:16.17 Positioning Slug by 0.324ml
@19:21:16.26 AS.seek 0.326ml
@19:26:42.59 delay 5 seconds
@19:26:47.62 ATV.dial ARV
@19:26:48.44 ASV.dial :PCRlock
@19:26:48.84 delay 5 seconds
@19:26:53.87 Running AARPP4 Profile58CIQ2 thermocycling
@19:26:53.88 <PCR> PCR.program Profile58CIQ2
@19:26:54.00 Profile58CIQ2 should complete in < 1:36:15
@19:26:54.01 PCR.run 2:05:18.75
@20:47:15.90 PCR.off
@20:47:16.46 <MFBPCR> Running AARPP4 MeltProfile58 thermocycling
@20:47:16.47 <PCR> PCR.program MeltProfile58
@20:47:16.95 MeltProfile58 should complete in < 12:33
@20:47:16.98 PCR.run 20:41.25
@20:54:49.43 PCR.off
@20:54:50.40 <MFBPCR> PCR for AARPP4 completed -- closed /var/log/esp/AARPP421mar2319h.pcr
@20:54:50.47 Cleaning PCR
@20:54:50.48 AFV.select :carrierSolution
@20:54:50.60 delay 2 seconds
@20:54:52.62 AS.reconfigure ASconfig
@20:54:52.68 AS.seek 0.5ml
@20:55:02.97 delay 1 second
@20:55:03.99 AFV.select :holdingCoil
@20:55:04.10 ASV.dial :waste1
@20:55:04.49 ATV.dial :air3
@20:55:05.84 AS.reconfigure ASslow1
@20:55:05.90 AS.seek 0.51ml
@20:55:08.17 delay 2 seconds
@20:55:10.20 ATV.dial :bleach
@20:55:10.84 AS.seek 0.81ml
@20:56:11.41 delay 3 seconds
@20:56:14.44 ATV.dial :air3
@20:56:15.02 delay 3 seconds
@20:56:18.09 AS.seek 0.835ml
@20:56:23.39 delay 3 seconds
@20:56:26.42 ATV.dial :bleach
@20:56:27.06 AS.seek 0.82ml
@20:56:30.36 delay 3 seconds
@20:56:33.39 ARV.dial :waste
@20:56:34.02 delay 3 seconds
@20:56:37.05 ATV.dial :ARV
@20:56:37.99 AS.reconfigure ASconfig
@20:56:38.05 AS.seek 0.47ml
@20:56:58.46 AFV.select :carrierSolution
@20:56:58.58 delay 2 seconds
@20:57:00.60 AS.seek 1ml
@20:57:31.35 delay 1 second
@20:57:32.37 AFV.select :holdingCoil
@20:57:32.48 delay 1 second
@20:57:33.50 AS.reconfigure ASfast
@20:57:33.55 AS.seek 0ml
@20:58:00.89 AFV.select :carrierSolution
@20:58:01.01 delay 2 seconds
@20:58:03.03 AS.reconfigure ASconfig
@20:58:03.08 AS.seek 1ml
@20:59:00.83 delay 1 second
@20:59:01.85 AFV.select :holdingCoil
@20:59:01.96 delay 1 second
@20:59:02.98 AS.reconfigure ASfast
@20:59:03.04 AS.seek 0ml
@20:59:30.38 AFV.select :carrierSolution
@20:59:30.50 delay 2 seconds
@20:59:32.52 AS.reconfigure ASconfig
@20:59:32.57 AS.seek 1ml
@21:00:30.36 delay 1 second
@21:00:31.38 AFV.select :holdingCoil
@21:00:31.49 delay 1 second
@21:00:32.51 AS.reconfigure ASfast
@21:00:32.57 AS.seek 0ml
@21:00:59.91 ARV.dial between carrier and air1
@21:01:00.43 ATV.dial :air3
@21:01:01.80 AS.reconfigure ASslow1
@21:01:01.85 AS.seek 0.01ml
@21:01:04.27 delay 2 seconds
@21:01:06.30 ATV.dial :bleach
@21:01:06.94 AS.seek 0.81ml
@21:03:48.13 delay 3 seconds
@21:03:51.16 ATV.dial :air3
@21:03:51.74 delay 3 seconds
@21:03:54.81 AS.seek 0.835ml
@21:04:00.07 delay 3 seconds
@21:04:03.10 ATV.dial :bleach
@21:04:03.74 AS.seek 0.82ml
@21:04:07.14 delay 3 seconds
@21:04:10.17 ATV.dial PCR
@21:04:10.59 AS.reconfigure ASconfig
@21:04:10.64 AS.seek 0ml
@21:04:58.03 PCR.program :bleachProfile
@21:04:58.11 PCR program should complete in < 2:45
@21:04:58.12 PCR.run 10:00
@21:07:54.33 PCR.off
@21:07:54.38 AFV.select :carrierSolution
@21:07:54.49 delay 2 seconds
@21:07:56.51 AS.seek 1ml
@21:08:54.30 delay 1 second
@21:08:55.32 AFV.select :holdingCoil
@21:08:55.43 delay 1 second
@21:08:56.45 AS.reconfigure ASfast
@21:08:56.51 AS.seek 0ml
@21:09:23.86 delay 3 seconds
@21:09:26.88 AFV.select :carrierSolution
@21:09:26.99 delay 2 seconds
@21:09:29.01 AS.reconfigure ASconfig
@21:09:29.07 AS.seek 1ml
@21:10:26.82 delay 1 second
@21:10:27.84 AFV.select :holdingCoil
@21:10:27.95 delay 1 second
@21:10:28.97 AS.reconfigure ASfast
@21:10:29.02 AS.seek 0ml
@21:10:56.35 delay 3 seconds
@21:10:59.37 AFV.select :carrierSolution
@21:10:59.48 delay 2 seconds
@21:11:01.50 AS.reconfigure ASconfig
@21:11:01.57 AS.seek 1ml
@21:11:59.35 delay 1 second
@21:12:00.37 AFV.select :holdingCoil
@21:12:00.48 delay 1 second
@21:12:01.50 AS.reconfigure ASfast
@21:12:01.56 AS.seek 0ml
@21:12:28.91 delay 3 seconds
@21:12:31.93 AFV.select :carrierSolution
@21:12:32.04 delay 2 seconds
@21:12:34.06 AS.reconfigure ASconfig
@21:12:34.13 AS.seek 1ml
@21:13:31.89 delay 1 second
@21:13:32.91 AFV.select :holdingCoil
@21:13:33.02 delay 1 second
@21:13:34.04 AS.reconfigure ASfast
@21:13:34.09 AS.seek 0ml
@21:14:01.43 delay 3 seconds
@21:14:04.45 AFV.select :carrierSolution
@21:14:04.56 delay 2 seconds
@21:14:06.58 AS.reconfigure ASconfig
@21:14:06.64 AS.seek 1ml
@21:15:04.39 delay 1 second
@21:15:05.41 AFV.select :holdingCoil
@21:15:05.52 delay 1 second
@21:15:06.54 AS.reconfigure ASnorm
@21:15:06.59 AS.seek 0ml
@21:15:57.30 Setting up PCR for AALPP3 -- writing /var/log/esp/AALPP321mar2321h.pcr
@21:15:57.31 Building PCR Slug from ASV with MM2 mix, PP3 primer
@21:15:57.34 Priming with carrier
@21:15:57.36 ATV.dial ARV
@21:15:58.00 ARV.dial :carrier
@21:15:58.33 AS.seek 0.5ml
@21:16:23.86 AFV.select :carrierSolution
@21:16:23.98 delay 2 seconds
@21:16:26.00 AS.reconfigure ASconfig
@21:16:26.06 AS.seek 1ml
@21:16:55.07 delay 1 second
@21:16:56.09 AFV.select :holdingCoil
@21:16:56.20 delay 1 second
@21:16:57.22 ATV.dial :waste
@21:16:59.00 AS.reconfigure ASnorm
@21:16:59.06 AS.seek 0ml
@21:17:49.75 AFV.select :carrierSolution
@21:17:49.87 delay 2 seconds
@21:17:51.89 AS.reconfigure ASconfig
@21:17:51.95 AS.seek 0.5ml
@21:18:20.99 delay 1 second
@21:18:22.01 AFV.select :holdingCoil
@21:18:22.12 Building Slug
@21:18:22.13 ATV.dial :ARV
@21:18:23.88 delay 2 seconds
@21:18:25.91 ARV.dial :air1
@21:18:26.30 delay 2 seconds
@21:18:28.37 AS.reconfigure ASslow2
@21:18:28.43 AS.seek 0.506ml
@21:18:34.85 delay 2 seconds
@21:18:36.88 ARV.dial :MM2
@21:18:38.44 delay 2 seconds
@21:18:40.46 Adding MM2
@21:18:40.53 AS.seek 0.524ml
@21:18:59.43 delay 25 seconds
@21:19:24.46 ARV.dial :PP3
@21:19:24.87 Adding PP3
@21:19:24.88 delay 2 seconds
@21:19:26.96 AS.seek 0.53ml
@21:19:33.21 delay 10 seconds
@21:19:43.25 ARV.dial :air2
@21:19:43.67 adding ARV air
@21:19:43.68 delay 2 seconds
@21:19:45.75 AS.seek 0.536ml
@21:19:52.07 delay 5 seconds
@21:19:57.10 ARV.dial :carrier
@21:19:58.43 delay 7 seconds
@21:20:05.51 AS.seek 0.594ml
@21:21:03.94 delay 5 seconds
@21:21:08.97 ATV.dial :ASV
@21:21:09.39 delay 2 seconds
@21:21:11.47 AS.seek 0.6ml
@21:21:17.79 delay 4 seconds
@21:21:21.82 ATV.dial ARV
@21:21:22.41 delay 2 seconds
@21:21:24.44 ARV.dial :carrier
@21:21:24.45 delay 2 seconds
@21:21:26.53 AS.seek 0.65ml
@21:22:17.41 delay 4 seconds
@21:22:21.44 ATV.dial PCR
@21:22:22.08 ASV.dial :waste1
@21:22:22.10 ARV.dial :air1
@21:22:22.51 Positioning Slug by 0.324ml
@21:22:22.60 AS.seek 0.326ml
@21:27:49.14 delay 5 seconds
@21:27:54.17 ATV.dial ARV
@21:27:54.97 ASV.dial :PCRlock
@21:27:55.38 delay 5 seconds
@21:28:00.41 Running AALPP3 Profile58CIQ2 thermocycling
@21:28:00.42 <PCR> PCR.program Profile58CIQ2
@21:28:00.54 Profile58CIQ2 should complete in < 1:36:15
@21:28:00.55 PCR.run 2:05:18.75
@22:48:23.26 PCR.off
@22:48:23.82 <MFBPCR> Running AALPP3 MeltProfile58 thermocycling
@22:48:23.84 <PCR> PCR.program MeltProfile58
@22:48:23.94 MeltProfile58 should complete in < 12:33
@22:48:23.95 PCR.run 20:41.25
@22:55:59.55 PCR.off
@22:56:00.51 <MFBPCR> PCR for AALPP3 completed -- closed /var/log/esp/AALPP321mar2321h.pcr
@22:56:00.59 Cleaning PCR
@22:56:00.60 AFV.select :carrierSolution
@22:56:00.72 delay 2 seconds
@22:56:02.74 AS.reconfigure ASconfig
@22:56:02.79 AS.seek 0.5ml
@22:56:13.08 delay 1 second
@22:56:14.10 AFV.select :holdingCoil
@22:56:14.21 ASV.dial :waste1
@22:56:14.60 ATV.dial :air3
@22:56:15.96 AS.reconfigure ASslow1
@22:56:16.02 AS.seek 0.51ml
@22:56:18.30 delay 2 seconds
@22:56:20.32 ATV.dial :bleach
@22:56:20.96 AS.seek 0.81ml
@22:57:21.55 delay 3 seconds
@22:57:24.58 ATV.dial :air3
@22:57:25.16 delay 3 seconds
@22:57:28.23 AS.seek 0.835ml
@22:57:33.55 delay 3 seconds
@22:57:36.58 ATV.dial :bleach
@22:57:37.22 AS.seek 0.82ml
@22:57:40.54 delay 3 seconds
@22:57:43.57 ARV.dial :waste
@22:57:44.19 delay 3 seconds
@22:57:47.22 ATV.dial :ARV
@22:57:48.14 AS.reconfigure ASconfig
@22:57:48.19 AS.seek 0.47ml
@22:58:08.62 AFV.select :carrierSolution
@22:58:08.74 delay 2 seconds
@22:58:10.76 AS.seek 1ml
@22:58:41.52 delay 1 second
@22:58:42.54 AFV.select :holdingCoil
@22:58:42.65 delay 1 second
@22:58:43.67 AS.reconfigure ASfast
@22:58:43.73 AS.seek 0ml
@22:59:11.07 AFV.select :carrierSolution
@22:59:11.19 delay 2 seconds
@22:59:13.21 AS.reconfigure ASconfig
@22:59:13.26 AS.seek 1ml
@23:00:11.02 delay 1 second
@23:00:12.04 AFV.select :holdingCoil
@23:00:12.15 delay 1 second
@23:00:13.17 AS.reconfigure ASfast
@23:00:13.23 AS.seek 0ml
@23:00:40.57 AFV.select :carrierSolution
@23:00:40.69 delay 2 seconds
@23:00:42.71 AS.reconfigure ASconfig
@23:00:42.76 AS.seek 1ml
@23:01:40.52 delay 1 second
@23:01:41.54 AFV.select :holdingCoil
@23:01:41.65 delay 1 second
@23:01:42.67 AS.reconfigure ASfast
@23:01:42.73 AS.seek 0ml
@23:02:10.07 ARV.dial between carrier and air1
@23:02:10.60 ATV.dial :air3
@23:02:11.97 AS.reconfigure ASslow1
@23:02:12.03 AS.seek 0.01ml
@23:02:14.33 delay 2 seconds
@23:02:16.36 ATV.dial :bleach
@23:02:17.02 AS.seek 0.81ml
@23:04:58.15 delay 3 seconds
@23:05:01.18 ATV.dial :air3
@23:05:01.76 delay 3 seconds
@23:05:05.24 AS.seek 0.835ml
@23:05:10.55 delay 3 seconds
@23:05:13.58 ATV.dial :bleach
@23:05:14.22 AS.seek 0.82ml
@23:05:17.60 delay 3 seconds
@23:05:20.63 ATV.dial PCR
@23:05:21.05 AS.reconfigure ASconfig
@23:05:21.10 AS.seek 0ml
@23:06:08.54 PCR.program :bleachProfile
@23:06:08.62 PCR program should complete in < 2:45
@23:06:08.63 PCR.run 10:00
@23:09:07.45 PCR.off
@23:09:07.49 AFV.select :carrierSolution
@23:09:07.59 delay 2 seconds
@23:09:09.61 AS.seek 1ml
@23:10:07.40 delay 1 second
@23:10:08.42 AFV.select :holdingCoil
@23:10:08.53 delay 1 second
@23:10:09.55 AS.reconfigure ASfast
@23:10:09.61 AS.seek 0ml
@23:10:36.96 delay 3 seconds
@23:10:39.98 AFV.select :carrierSolution
@23:10:40.09 delay 2 seconds
@23:10:42.11 AS.reconfigure ASconfig
@23:10:42.16 AS.seek 1ml
@23:11:39.94 delay 1 second
@23:11:40.96 AFV.select :holdingCoil
@23:11:41.07 delay 1 second
@23:11:42.09 AS.reconfigure ASfast
@23:11:42.15 AS.seek 0ml
@23:12:09.49 delay 3 seconds
@23:12:12.51 AFV.select :carrierSolution
@23:12:12.62 delay 2 seconds
@23:12:14.64 AS.reconfigure ASconfig
@23:12:14.71 AS.seek 1ml
@23:13:12.46 delay 1 second
@23:13:13.48 AFV.select :holdingCoil
@23:13:13.59 delay 1 second
@23:13:14.61 AS.reconfigure ASfast
@23:13:14.67 AS.seek 0ml
@23:13:42.01 delay 3 seconds
@23:13:45.03 AFV.select :carrierSolution
@23:13:45.14 delay 2 seconds
@23:13:47.16 AS.reconfigure ASconfig
@23:13:47.21 AS.seek 1ml
@23:14:45.01 delay 1 second
@23:14:46.03 AFV.select :holdingCoil
@23:14:46.14 delay 1 second
@23:14:47.16 AS.reconfigure ASfast
@23:14:47.22 AS.seek 0ml
@23:15:14.55 delay 3 seconds
@23:15:17.57 AFV.select :carrierSolution
@23:15:17.68 delay 2 seconds
@23:15:19.70 AS.reconfigure ASconfig
@23:15:19.77 AS.seek 1ml
@23:16:17.52 delay 1 second
@23:16:18.54 AFV.select :holdingCoil
@23:16:18.65 delay 1 second
@23:16:19.67 AS.reconfigure ASnorm
@23:16:19.73 AS.seek 0ml
@23:17:10.46 Setting up PCR for ACOPP5 -- writing /var/log/esp/ACOPP521mar2323h.pcr
@23:17:10.47 Building PCR Slug from ASV with MM3 mix, PP5 primer
@23:17:10.50 Priming with carrier
@23:17:10.52 ATV.dial ARV
@23:17:11.18 ARV.dial :carrier
@23:17:11.54 AS.seek 0.5ml
@23:17:37.11 AFV.select :carrierSolution
@23:17:37.24 delay 2 seconds
@23:17:39.26 AS.reconfigure ASconfig
@23:17:39.33 AS.seek 1ml
@23:18:08.35 delay 1 second
@23:18:09.37 AFV.select :holdingCoil
@23:18:09.48 delay 1 second
@23:18:10.50 ATV.dial :waste
@23:18:12.28 AS.reconfigure ASnorm
@23:18:12.34 AS.seek 0ml
@23:19:03.05 AFV.select :carrierSolution
@23:19:03.18 delay 2 seconds
@23:19:05.20 AS.reconfigure ASconfig
@23:19:05.26 AS.seek 0.5ml
@23:19:34.27 delay 1 second
@23:19:35.29 AFV.select :holdingCoil
@23:19:35.40 Building Slug
@23:19:35.41 ATV.dial :ARV
@23:19:37.16 delay 2 seconds
@23:19:39.19 ARV.dial :air1
@23:19:39.60 delay 2 seconds
@23:19:41.67 AS.reconfigure ASslow2
@23:19:41.73 AS.seek 0.506ml
@23:19:48.15 delay 2 seconds
@23:19:50.18 ARV.dial :MM3
@23:19:51.26 delay 2 seconds
@23:19:53.29 Adding MM3
@23:19:53.37 AS.seek 0.524ml
@23:20:11.74 delay 25 seconds
@23:20:36.77 ARV.dial :PP5
@23:20:37.19 Adding PP5
@23:20:37.20 delay 2 seconds
@23:20:39.27 AS.seek 0.53ml
@23:20:45.55 delay 10 seconds
@23:20:55.59 ARV.dial :air3
@23:20:56.00 adding ARV air
@23:20:56.01 delay 2 seconds
@23:20:58.08 AS.seek 0.536ml
@23:21:04.62 delay 5 seconds
@23:21:09.65 ARV.dial :carrier
@23:21:10.96 delay 7 seconds
@23:21:18.04 AS.seek 0.594ml
@23:22:16.57 delay 5 seconds
@23:22:21.60 ATV.dial :ASV
@23:22:22.02 delay 2 seconds
@23:22:24.09 AS.seek 0.6ml
@23:22:30.55 delay 4 seconds
@23:22:34.58 ATV.dial ARV
@23:22:35.16 delay 2 seconds
@23:22:37.19 ARV.dial :carrier
@23:22:37.20 delay 2 seconds
@23:22:39.27 AS.seek 0.65ml
@23:23:29.90 delay 4 seconds
@23:23:33.93 ATV.dial PCR
@23:23:34.57 ASV.dial :waste1
@23:23:34.59 ARV.dial :air1
@23:23:35.02 Positioning Slug by 0.324ml
@23:23:35.11 AS.seek 0.326ml
@23:29:01.16 delay 5 seconds
@23:29:06.19 ATV.dial ARV
@23:29:06.99 ASV.dial :PCRlock
@23:29:07.40 delay 5 seconds
@23:29:12.43 Running ACOPP5 ProfileIPc thermocycling
@23:29:12.44 <PCR> PCR.program ProfileIPc
@23:29:12.56 ProfileIPc should complete in < 1:26:35
@23:29:12.57 PCR.run 1:53:13.75
@00:37:26.02CET24-Mar-21 PCR.off
@00:37:26.58 <MFBPCR> PCR for ACOPP5 completed -- closed /var/log/esp/ACOPP521mar2323h.pcr
@00:37:26.65 Cleaning PCR
@00:37:26.66 AFV.select :carrierSolution
@00:37:26.78 delay 2 seconds
@00:37:28.80 AS.reconfigure ASconfig
@00:37:28.85 AS.seek 0.5ml
@00:37:39.15 delay 1 second
@00:37:40.17 AFV.select :holdingCoil
@00:37:40.28 ASV.dial :waste1
@00:37:40.67 ATV.dial :air3
@00:37:42.04 AS.reconfigure ASslow1
@00:37:42.10 AS.seek 0.51ml
@00:37:44.38 delay 2 seconds
@00:37:46.41 ATV.dial :bleach
@00:37:47.07 AS.seek 0.81ml
@00:38:47.65 delay 3 seconds
@00:38:50.68 ATV.dial :air3
@00:38:51.28 delay 3 seconds
@00:38:54.35 AS.seek 0.835ml
@00:38:59.64 delay 3 seconds
@00:39:02.67 ATV.dial :bleach
@00:39:03.30 AS.seek 0.82ml
@00:39:06.57 delay 3 seconds
@00:39:09.60 ARV.dial :waste
@00:39:10.23 delay 3 seconds
@00:39:13.26 ATV.dial :ARV
@00:39:14.19 AS.reconfigure ASconfig
@00:39:14.24 AS.seek 0.47ml
@00:39:34.65 AFV.select :carrierSolution
@00:39:34.77 delay 2 seconds
@00:39:36.79 AS.seek 1ml
@00:40:07.54 delay 1 second
@00:40:08.56 AFV.select :holdingCoil
@00:40:08.67 delay 1 second
@00:40:09.69 AS.reconfigure ASfast
@00:40:09.75 AS.seek 0ml
@00:40:37.06 AFV.select :carrierSolution
@00:40:37.18 delay 2 seconds
@00:40:39.20 AS.reconfigure ASconfig
@00:40:39.25 AS.seek 1ml
@00:41:36.99 delay 1 second
@00:41:38.01 AFV.select :holdingCoil
@00:41:38.12 delay 1 second
@00:41:39.14 AS.reconfigure ASfast
@00:41:39.20 AS.seek 0ml
@00:42:06.51 AFV.select :carrierSolution
@00:42:06.63 delay 2 seconds
@00:42:08.65 AS.reconfigure ASconfig
@00:42:08.70 AS.seek 1ml
@00:43:06.46 delay 1 second
@00:43:07.48 AFV.select :holdingCoil
@00:43:07.59 delay 1 second
@00:43:08.61 AS.reconfigure ASfast
@00:43:08.67 AS.seek 0ml
@00:43:35.99 ARV.dial between carrier and air1
@00:43:36.49 ATV.dial :air3
@00:43:37.87 AS.reconfigure ASslow1
@00:43:37.93 AS.seek 0.01ml
@00:43:40.23 delay 2 seconds
@00:43:42.26 ATV.dial :bleach
@00:43:42.91 AS.seek 0.81ml
@00:46:24.45 delay 3 seconds
@00:46:27.48 ATV.dial :air3
@00:46:28.07 delay 3 seconds
@00:46:31.14 AS.seek 0.835ml
@00:46:36.46 delay 3 seconds
@00:46:39.49 ATV.dial :bleach
@00:46:40.13 AS.seek 0.82ml
@00:46:43.42 delay 3 seconds
@00:46:46.45 ATV.dial PCR
@00:46:46.86 AS.reconfigure ASconfig
@00:46:46.91 AS.seek 0ml
@00:47:34.32 PCR.program :bleachProfile
@00:47:34.40 PCR program should complete in < 2:45
@00:47:34.41 PCR.run 10:00
@00:50:33.23 PCR.off
@00:50:33.27 AFV.select :carrierSolution
@00:50:33.39 delay 2 seconds
@00:50:35.41 AS.seek 1ml
@00:51:33.21 delay 1 second
@00:51:34.23 AFV.select :holdingCoil
@00:51:34.34 delay 1 second
@00:51:35.36 AS.reconfigure ASfast
@00:51:35.42 AS.seek 0ml
@00:52:02.76 delay 3 seconds
@00:52:05.78 AFV.select :carrierSolution
@00:52:05.89 delay 2 seconds
@00:52:07.91 AS.reconfigure ASconfig
@00:52:07.97 AS.seek 1ml
@00:53:05.76 delay 1 second
@00:53:06.78 AFV.select :holdingCoil
@00:53:06.89 delay 1 second
@00:53:07.91 AS.reconfigure ASfast
@00:53:07.97 AS.seek 0ml
@00:53:35.31 delay 3 seconds
@00:53:38.33 AFV.select :carrierSolution
@00:53:38.44 delay 2 seconds
@00:53:40.46 AS.reconfigure ASconfig
@00:53:40.53 AS.seek 1ml
@00:54:38.31 delay 1 second
@00:54:39.33 AFV.select :holdingCoil
@00:54:39.44 delay 1 second
@00:54:40.46 AS.reconfigure ASfast
@00:54:40.52 AS.seek 0ml
@00:55:07.87 delay 3 seconds
@00:55:10.89 AFV.select :carrierSolution
@00:55:11.00 delay 2 seconds
@00:55:13.02 AS.reconfigure ASconfig
@00:55:13.08 AS.seek 1ml
@00:56:10.87 delay 1 second
@00:56:11.89 AFV.select :holdingCoil
@00:56:12.00 delay 1 second
@00:56:13.02 AS.reconfigure ASfast
@00:56:13.08 AS.seek 0ml
@00:56:40.43 delay 3 seconds
@00:56:43.45 AFV.select :carrierSolution
@00:56:43.56 delay 2 seconds
@00:56:45.58 AS.reconfigure ASconfig
@00:56:45.65 AS.seek 1ml
@00:57:43.40 delay 1 second
@00:57:44.42 AFV.select :holdingCoil
@00:57:44.53 delay 1 second
@00:57:45.55 AS.reconfigure ASnorm
@00:57:45.60 AS.seek 0ml
@00:58:36.32 Setting up PCR for AIPc -- writing /var/log/esp/AIPc21mar2400h.pcr
@00:58:36.33 Building PCR Slug from ASV with MM3 mix, PP6 primer
@00:58:36.36 Priming with carrier
@00:58:36.37 ATV.dial ARV
@00:58:37.43 ARV.dial :carrier
@00:58:37.77 AS.seek 0.5ml
@00:59:03.34 AFV.select :carrierSolution
@00:59:03.47 delay 2 seconds
@00:59:05.49 AS.reconfigure ASconfig
@00:59:05.56 AS.seek 1ml
@00:59:34.57 delay 1 second
@00:59:35.59 AFV.select :holdingCoil
@00:59:35.70 delay 1 second
@00:59:36.72 ATV.dial :waste
@00:59:38.50 AS.reconfigure ASnorm
@00:59:38.56 AS.seek 0ml
@01:00:29.28 AFV.select :carrierSolution
@01:00:29.41 delay 2 seconds
@01:00:31.43 AS.reconfigure ASconfig
@01:00:31.49 AS.seek 0.5ml
@01:01:00.53 delay 1 second
@01:01:01.55 AFV.select :holdingCoil
@01:01:01.66 Building Slug
@01:01:01.67 ATV.dial :ARV
@01:01:03.42 delay 2 seconds
@01:01:05.45 ARV.dial :air1
@01:01:05.85 delay 2 seconds
@01:01:07.92 AS.reconfigure ASslow2
@01:01:07.98 AS.seek 0.506ml
@01:01:14.48 delay 2 seconds
@01:01:16.51 ARV.dial :MM3
@01:01:17.59 delay 2 seconds
@01:01:19.62 Adding MM3
@01:01:19.70 AS.seek 0.524ml
@01:01:38.40 delay 25 seconds
@01:02:03.43 ARV.dial :PP6
@01:02:03.83 Adding PP6
@01:02:03.84 delay 2 seconds
@01:02:05.91 AS.seek 0.53ml
@01:02:12.29 delay 10 seconds
@01:02:22.33 ARV.dial :air1
@01:02:23.19 adding ARV air
@01:02:23.20 delay 2 seconds
@01:02:25.27 AS.seek 0.536ml
@01:02:31.64 delay 5 seconds
@01:02:36.67 ARV.dial :carrier
@01:02:37.07 delay 7 seconds
@01:02:44.14 AS.seek 0.594ml
@01:03:42.65 delay 5 seconds
@01:03:47.68 ATV.dial :ASV
@01:03:48.09 delay 2 seconds
@01:03:50.17 AS.seek 0.6ml
@01:03:56.45 delay 4 seconds
@01:04:00.48 ATV.dial ARV
@01:04:01.06 delay 2 seconds
@01:04:03.09 ARV.dial :carrier
@01:04:03.10 delay 2 seconds
@01:04:05.17 AS.seek 0.65ml
@01:04:55.76 delay 4 seconds
@01:04:59.79 ATV.dial PCR
@01:05:00.43 ASV.dial :waste1
@01:05:00.45 ARV.dial :air1
@01:05:00.87 Positioning Slug by 0.324ml
@01:05:00.95 AS.seek 0.326ml
@01:10:27.10 delay 5 seconds
@01:10:32.13 ATV.dial ARV
@01:10:32.96 ASV.dial :PCRlock
@01:10:33.35 delay 5 seconds
@01:10:38.38 Running AIPc ProfileIPc thermocycling
@01:10:38.39 <PCR> PCR.program ProfileIPc
@01:10:38.52 ProfileIPc should complete in < 1:26:35
@01:10:38.53 PCR.run 1:53:13.75
@02:18:39.05 PCR.off
@02:18:39.61 <MFBPCR> PCR for AIPc completed -- closed /var/log/esp/AIPc21mar2400h.pcr
@02:18:39.63 Full MFB Decontamination
@02:18:39.65 Decon extraction column and decon extSrc
@02:18:39.68 SPE.hold 2:46:40,55C
@02:18:39.72 ASV.dial :SPEdelivery
@02:18:40.40 delay 20 seconds
@02:19:00.43 AFV.select :carrierSolution
@02:19:00.54 delay 2 seconds
@02:19:02.56 AS.reconfigure ASconfig
@02:19:02.61 AS.seek 0.5ml
@02:19:12.93 delay 1 second
@02:19:13.95 AFV.select :holdingCoil
@02:19:14.06 ATV.dial :air1
@02:19:16.12 AS.reconfigure ASslow1
@02:19:16.18 AS.seek 0.52ml
@02:19:20.48 ATV.dial :ASV
@02:19:22.42 ASV.dial :SPErecovery
@02:19:23.12 AS.seek 0.62ml
@02:19:43.50 ATV.dial :waste
@02:19:45.73 AS.reconfigure ASnorm
@02:19:45.79 AS.seek 0ml
@02:20:17.39 deconEluateRecoveryLine extSrc
@02:20:17.40 ATV.dial :waste
@02:20:17.44 AS.seek 0ml
@02:20:17.52 ATV.dial :air3
@02:20:18.29 AS.reconfigure ASslow1
@02:20:18.35 AS.seek 0.01ml
@02:20:20.65 delay 2 seconds
@02:20:22.68 ATV.dial :bleach
@02:20:23.18 AS.seek 0.31ml
@02:21:23.68 delay 3 seconds
@02:21:26.71 ATV.dial :air3
@02:21:27.34 delay 3 seconds
@02:21:30.41 AS.seek 0.335ml
@02:21:35.65 delay 3 seconds
@02:21:38.68 ATV.dial :bleach
@02:21:39.35 AS.seek 0.32ml
@02:21:42.64 delay 3 seconds
@02:21:45.67 ATV.dial :extSrc
@02:21:46.37 AS.seek 0ml
@02:22:50.97 AFV.select :carrierSolution
@02:22:51.09 delay 2 seconds
@02:22:53.11 AS.reconfigure ASconfig
@02:22:53.17 AS.seek 0.5ml
@02:23:22.22 delay 1 second
@02:23:23.24 AFV.select :holdingCoil
@02:23:23.35 ATV.dial :extSrc
@02:23:23.37 AS.reconfigure ASslow1
@02:23:23.43 AS.seek 0ml
@02:25:04.26 AFV.select :carrierSolution
@02:25:04.38 delay 2 seconds
@02:25:06.40 AS.reconfigure ASconfig
@02:25:06.46 AS.seek 0.5ml
@02:25:35.50 delay 1 second
@02:25:36.52 AFV.select :holdingCoil
@02:25:36.63 ATV.dial :extSrc
@02:25:36.65 AS.reconfigure ASslow1
@02:25:36.71 AS.seek 0ml
@02:27:17.51 AFV.select :carrierSolution
@02:27:18.04 delay 2 seconds
@02:27:20.06 AS.reconfigure ASconfig
@02:27:20.12 AS.seek 0.5ml
@02:27:49.15 delay 1 second
@02:27:50.17 AFV.select :holdingCoil
@02:27:50.28 ATV.dial :extSrc
@02:27:50.30 AS.reconfigure ASslow1
@02:27:50.37 AS.seek 0ml
@02:29:31.19 AFV.select :carrierSolution
@02:29:31.32 delay 2 seconds
@02:29:33.34 AS.reconfigure ASconfig
@02:29:33.40 AS.seek 0.5ml
@02:30:02.44 delay 1 second
@02:30:03.46 AFV.select :holdingCoil
@02:30:03.57 ATV.dial :extSrc
@02:30:03.59 AS.reconfigure ASslow1
@02:30:03.66 AS.seek 0ml
@02:31:44.64 ATV.dial :air2
@02:31:46.03 AS.reconfigure ASslow0
@02:31:46.09 AS.seek 0.5ml
@02:32:36.78 ATV.dial :extSrc
@02:32:38.36 AS.seek 0.02ml
@02:33:26.95 delay 1 second
@02:33:27.98 ATV.dial :waste
@02:33:29.34 AS.reconfigure ASconfig
@02:33:29.39 AS.seek 0ml
@02:33:30.84 AFV.select :holdingCoil
@02:33:30.86 ATV.dial :waste
@02:33:30.88 AS.seek 0ml
@02:33:30.96 AFV.select :carrierSolution
@02:33:31.07 delay 2 seconds
@02:33:33.09 AS.seek 0.5ml
@02:34:02.17 delay 1 second
@02:34:03.19 AFV.select :holdingCoil
@02:34:03.30 ATV.dial :air3
@02:34:04.17 AS.reconfigure ASslow1
@02:34:04.23 AS.seek 0.52ml
@02:34:08.53 ATV.dial :air3
@02:34:08.62 AS.seek 0.53ml
@02:34:10.90 delay 2 seconds
@02:34:12.93 ATV.dial :bleach
@02:34:13.42 AS.seek 0.71ml
@02:34:49.87 delay 3 seconds
@02:34:52.90 ATV.dial :air3
@02:34:53.48 delay 3 seconds
@02:34:56.55 AS.seek 0.735ml
@02:35:01.85 delay 3 seconds
@02:35:04.88 ATV.dial :bleach
@02:35:05.51 AS.seek 0.72ml
@02:35:08.79 delay 3 seconds
@02:35:11.82 ATV.dial ASV
@02:35:12.92 ASV.dial :SPErecovery
@02:35:13.01 AS.seek 0.365ml
@02:36:24.62 ATV.dial :waste
@02:36:26.63 AS.reconfigure ASnorm
@02:36:26.69 AS.seek 0ml
@02:36:45.40 ATV.dial :air3
@02:36:46.28 AS.reconfigure ASslow1
@02:36:46.34 AS.seek 0.01ml
@02:36:48.78 delay 2 seconds
@02:36:50.81 ATV.dial :bleach
@02:36:51.31 AS.seek 0.58ml
@02:38:46.25 delay 3 seconds
@02:38:49.28 ATV.dial :air3
@02:38:49.86 delay 3 seconds
@02:38:52.93 AS.seek 0.605ml
@02:38:58.34 delay 3 seconds
@02:39:01.37 ATV.dial :bleach
@02:39:02.01 AS.seek 0.59ml
@02:39:05.31 delay 3 seconds
@02:39:08.34 ATV.dial :ASV
@02:39:09.44 ASV.dial :wasteR
@02:39:09.85 AS.seek 0ml
@02:41:08.77 delay 1:00
@02:42:08.80 ATV.dial ASV
@02:42:08.81 AFV.select :carrierSolution
@02:42:08.93 delay 2 seconds
@02:42:10.95 AS.reconfigure ASconfig
@02:42:11.00 AS.seek 1ml
@02:43:08.78 delay 1 second
@02:43:09.80 AFV.select :holdingCoil
@02:43:09.91 delay 1 second
@02:43:10.93 ASV.dial :wasteR
@02:43:10.94 AS.reconfigure ASslow1
@02:43:11.01 AS.seek 0ml
@02:46:32.39 delay 10 seconds
@02:46:42.43 ATV.dial :air3
@02:46:44.01 AS.seek 0.01ml
@02:46:46.40 delay 2 seconds
@02:46:48.43 ATV.dial :bleach
@02:46:49.07 AS.seek 0.58ml
@02:48:44.01 delay 3 seconds
@02:48:47.04 ATV.dial :air3
@02:48:47.62 delay 3 seconds
@02:48:50.69 AS.seek 0.605ml
@02:48:56.01 delay 3 seconds
@02:48:59.04 ATV.dial :bleach
@02:48:59.68 AS.seek 0.59ml
@02:49:02.98 delay 3 seconds
@02:49:06.01 ATV.dial ASV
@02:49:07.12 ASV.dial :SPEdelivery
@02:49:07.51 AS.seek 0ml
@02:51:06.40 delay 1:00
@02:52:06.43 AFV.select :carrierSolution
@02:52:06.54 delay 2 seconds
@02:52:08.56 AS.reconfigure ASconfig
@02:52:08.62 AS.seek 1ml
@02:53:06.81 delay 1 second
@02:53:07.82 AFV.select :holdingCoil
@02:53:07.93 delay 1 second
@02:53:08.95 ATV.dial ASV
@02:53:08.96 ASV.dial :SPEdelivery
@02:53:08.98 AS.reconfigure ASslow1
@02:53:09.06 AS.seek 0ml
@02:56:30.45 delay 10 seconds
@02:56:40.49 ATV.dial :air2
@02:56:41.37 AS.reconfigure ASslow0
@02:56:41.43 AS.seek 0.8ml
@02:58:02.23 delay 3 seconds
@02:58:05.26 ATV.dial :ASV
@02:58:06.33 ASV.dial :SPEdelivery
@02:58:06.35 AS.reconfigure ASslow1
@02:58:06.42 AS.seek 0.03ml
@03:00:41.56 delay 20 seconds
@03:01:01.60 ATV.dial :waste,via: :air3
@03:01:03.43 AS.reconfigure ASconfig
@03:01:03.48 AS.seek 0ml
@03:01:05.51 SPE.coast
@03:01:05.59 Removing Bleach from Mixing Coil
@03:01:05.60 AFV.select :carrierSolution
@03:01:05.73 delay 2 seconds
@03:01:07.75 AS.seek 0.5ml
@03:01:36.76 delay 1 second
@03:01:37.78 AFV.select :holdingCoil
@03:01:37.89 ATV.dial :ASV
@03:01:39.70 ASV.dial :SPErecovery
@03:01:40.36 AS.reconfigure ASslow1
@03:01:40.42 AS.seek 0.73ml
@03:02:26.98 ASV.dial :wasteD
@03:02:27.59 AS.reconfigure ASnorm
@03:02:27.64 AS.seek 0ml
@03:03:04.72 AFV.select :carrierSolution
@03:03:04.83 delay 2 seconds
@03:03:06.86 AS.reconfigure ASconfig
@03:03:06.98 AS.seek 0.5ml
@03:03:36.03 delay 1 second
@03:03:37.05 AFV.select :holdingCoil
@03:03:37.16 ATV.dial ASV
@03:03:37.17 ASV.dial :SPErecovery
@03:03:37.84 AS.reconfigure ASslow0
@03:03:37.90 AS.seek 0.3ml
@03:03:58.34 ATV.dial :air2
@03:03:59.43 AS.reconfigure ASslow1
@03:03:59.49 AS.seek 0.32ml
@03:04:03.81 ATV.dial ASV
@03:04:04.92 AS.reconfigure ASslow0
@03:04:04.98 AS.seek 0.57ml
@03:04:30.45 ASV.dial :wasteD
@03:04:31.06 AS.reconfigure ASnorm
@03:04:31.12 AS.seek 0ml
@03:05:00.17 AFV.select :carrierSolution
@03:05:00.29 delay 2 seconds
@03:05:02.31 AS.reconfigure ASconfig
@03:05:02.36 AS.seek 0.5ml
@03:05:31.41 delay 1 second
@03:05:32.44 AFV.select :holdingCoil
@03:05:32.55 ATV.dial ASV
@03:05:32.56 ASV.dial :SPErecovery
@03:05:33.23 AS.reconfigure ASslow0
@03:05:33.29 AS.seek 0.3ml
@03:05:53.72 ATV.dial :air2
@03:05:54.82 AS.reconfigure ASslow1
@03:05:54.88 AS.seek 0.32ml
@03:05:59.22 ATV.dial ASV
@03:06:00.32 AS.reconfigure ASslow0
@03:06:00.38 AS.seek 0.57ml
@03:06:25.87 ASV.dial :wasteD
@03:06:26.48 AS.reconfigure ASnorm
@03:06:26.53 AS.seek 0ml
@03:06:55.61 ATV.dial :air2
@03:06:56.67 AS.reconfigure ASslow0
@03:06:56.73 AS.seek 0.3ml
@03:07:27.20 ATV.dial :ASV
@03:07:28.23 ASV.dial :wasteD
@03:07:28.26 AS.reconfigure ASconfig
@03:07:28.32 AS.seek 0.05ml
@03:07:43.00 ATV.dial :waste
@03:07:45.01 AS.seek 0ml
@03:07:48.17 ASV.dial :SPEdelivery
@03:07:49.27 Cleaning PCR
@03:07:49.28 AFV.select :carrierSolution
@03:07:49.41 delay 2 seconds
@03:07:51.43 AS.seek 0.5ml
@03:08:20.45 delay 1 second
@03:08:21.47 AFV.select :holdingCoil
@03:08:21.58 ASV.dial :waste1
@03:08:21.95 ATV.dial :air3
@03:08:22.67 AS.reconfigure ASslow1
@03:08:22.73 AS.seek 0.51ml
@03:08:25.09 delay 2 seconds
@03:08:27.12 ATV.dial :bleach
@03:08:27.59 AS.seek 0.81ml
@03:09:28.17 delay 3 seconds
@03:09:31.20 ATV.dial :air3
@03:09:31.78 delay 3 seconds
@03:09:34.85 AS.seek 0.835ml
@03:09:40.16 delay 3 seconds
@03:09:43.19 ATV.dial :bleach
@03:09:43.83 AS.seek 0.82ml
@03:09:47.12 delay 3 seconds
@03:09:50.15 ARV.dial :waste
@03:09:50.78 delay 3 seconds
@03:09:53.81 ATV.dial :ARV
@03:09:55.16 AS.reconfigure ASconfig
@03:09:55.22 AS.seek 0.47ml
@03:10:15.64 AFV.select :carrierSolution
@03:10:15.76 delay 2 seconds
@03:10:17.78 AS.seek 1ml
@03:10:48.56 delay 1 second
@03:10:49.58 AFV.select :holdingCoil
@03:10:49.69 delay 1 second
@03:10:50.71 AS.reconfigure ASfast
@03:10:50.77 AS.seek 0ml
@03:11:18.12 AFV.select :carrierSolution
@03:11:18.24 delay 2 seconds
@03:11:20.26 AS.reconfigure ASconfig
@03:11:20.31 AS.seek 1ml
@03:12:18.06 delay 1 second
@03:12:19.08 AFV.select :holdingCoil
@03:12:19.19 delay 1 second
@03:12:20.21 AS.reconfigure ASfast
@03:12:20.26 AS.seek 0ml
@03:12:47.62 AFV.select :carrierSolution
@03:12:47.74 delay 2 seconds
@03:12:49.76 AS.reconfigure ASconfig
@03:12:49.81 AS.seek 1ml
@03:13:47.55 delay 1 second
@03:13:48.57 AFV.select :holdingCoil
@03:13:48.68 delay 1 second
@03:13:49.70 AS.reconfigure ASfast
@03:13:49.76 AS.seek 0ml
@03:14:17.11 ARV.dial between carrier and air1
@03:14:17.63 ATV.dial :air3
@03:14:18.98 AS.reconfigure ASslow1
@03:14:19.04 AS.seek 0.01ml
@03:14:21.45 delay 2 seconds
@03:14:23.48 ATV.dial :bleach
@03:14:24.12 AS.seek 0.81ml
@03:17:05.31 delay 3 seconds
@03:17:08.34 ATV.dial :air3
@03:17:08.92 delay 3 seconds
@03:17:11.99 AS.seek 0.835ml
@03:17:17.28 delay 3 seconds
@03:17:20.31 ATV.dial :bleach
@03:17:20.95 AS.seek 0.82ml
@03:17:24.36 delay 3 seconds
@03:17:27.39 ATV.dial PCR
@03:17:27.82 AS.reconfigure ASconfig
@03:17:27.87 AS.seek 0ml
@03:18:15.30 PCR.program :bleachProfile
@03:18:15.37 PCR program should complete in < 2:45
@03:18:15.38 PCR.run 10:00
@03:21:17.23 PCR.off
@03:21:17.28 AFV.select :carrierSolution
@03:21:17.39 delay 2 seconds
@03:21:19.41 AS.seek 1ml
@03:22:17.17 delay 1 second
@03:22:18.19 AFV.select :holdingCoil
@03:22:18.30 delay 1 second
@03:22:19.32 AS.reconfigure ASfast
@03:22:19.38 AS.seek 0ml
@03:22:47.14 delay 3 seconds
@03:22:50.16 AFV.select :carrierSolution
@03:22:50.27 delay 2 seconds
@03:22:52.29 AS.reconfigure ASconfig
@03:22:52.35 AS.seek 1ml
@03:23:50.11 delay 1 second
@03:23:51.13 AFV.select :holdingCoil
@03:23:51.24 delay 1 second
@03:23:52.26 AS.reconfigure ASfast
@03:23:52.32 AS.seek 0ml
@03:24:19.66 delay 3 seconds
@03:24:22.68 AFV.select :carrierSolution
@03:24:22.79 delay 2 seconds
@03:24:24.81 AS.reconfigure ASconfig
@03:24:24.87 AS.seek 1ml
@03:25:22.66 delay 1 second
@03:25:23.68 AFV.select :holdingCoil
@03:25:23.79 delay 1 second
@03:25:24.81 AS.reconfigure ASfast
@03:25:24.87 AS.seek 0ml
@03:25:52.22 delay 3 seconds
@03:25:55.24 AFV.select :carrierSolution
@03:25:55.35 delay 2 seconds
@03:25:57.37 AS.reconfigure ASconfig
@03:25:57.43 AS.seek 1ml
@03:26:55.19 delay 1 second
@03:26:56.21 AFV.select :holdingCoil
@03:26:56.32 delay 1 second
@03:26:57.34 AS.reconfigure ASfast
@03:26:57.40 AS.seek 0ml
@03:27:24.75 delay 3 seconds
@03:27:27.77 AFV.select :carrierSolution
@03:27:27.88 delay 2 seconds
@03:27:29.90 AS.reconfigure ASconfig
@03:27:29.96 AS.seek 1ml
@03:28:27.73 delay 1 second
@03:28:28.75 AFV.select :holdingCoil
@03:28:28.86 delay 1 second
@03:28:29.88 AS.reconfigure ASnorm
@03:28:29.93 AS.seek 0ml
@03:29:20.64 AFV.select :carrierSolution
@03:29:20.75 delay 2 seconds
@03:29:22.77 AS.reconfigure ASconfig
@03:29:22.83 AS.seek 1ml
@03:30:20.58 delay 1 second
@03:30:21.60 AFV.select :holdingCoil
@03:30:21.71 delay 1 second
@03:30:22.73 ATV.dial :waste
@03:30:24.01 AS.reconfigure ASnorm
@03:30:24.06 AS.seek 0.5ml
@03:30:49.63 ATV.dial :waste2
@03:30:50.99 AS.seek 0ml
@03:31:16.57 <mwj> Session Ended
@12:09:01.06CET24-Mar-21 <MAIN> ESP2 v3.30 I2C Gateway v3.06 for core bus
@12:09:04.01 
Configuration for mer 7/20/20 brent@mbari.org
/home/esp/esp2/type/shallow/mer/configure.rb
@12:09:04.10 
Configuration for MFB3 by preston@mbari.org
/home/esp/esp2/type/mfb/MFB3/configure.rb
@12:09:10.14 Polling Can every 10:00
@12:09:12.10 CS.configure :default
@12:09:12.14 CS.hold
@12:09:12.20 CS.configure :default
@12:09:12.28 PS.configure :default
@12:09:12.36 SS.configure :default
@12:09:12.41 SS.hold
@12:09:12.49 SS.configure :default
@12:09:12.66 CC.configure :default
@12:09:12.73 CC.hold
@12:09:12.82 CC.configure :default
@12:09:12.98 PC.configure :default
@12:09:13.06 PC.hold
@12:09:13.17 PC.configure :default
@12:09:13.28 Forearm.configure :loose
@12:09:13.32 Forearm.hold
@12:09:13.36 Forearm.configure :loose
@12:09:13.44 Elbow.configure :loose
@12:09:13.48 Elbow.hold
@12:09:13.52 Elbow.configure :loose
@12:09:13.62 SC.configure :loose
@12:09:13.66 SC.hold
@12:09:13.70 SC.configure :loose
@12:09:13.83 SE.configure :default
@12:09:13.90 SE.hold
@12:09:14.00 SE.configure :default
@12:09:14.08 CH.coast
@12:09:14.17 CH.configure :default
@12:09:14.22 PH.coast
@12:09:14.32 PH.configure :default
@12:09:14.51 SPE.coast
@12:09:14.58 SPE.configure SPEconfig
@12:09:14.66 AS.configure ASconfig
@12:09:14.69 AS.hold
@12:09:14.75 AS.configure ASconfig
@12:09:14.80 PCR.off
@12:09:14.83 PCR.pidtemp= 50,0.1,0,300,25,25
@12:09:14.86 PCR.caltemp= 2.5051,0.0372,0
@12:09:14.89 All dwarf controllers running firmware version 3.75
@12:09:15.08 Cleanly exited at 12:08:45CET24-Mar-21
@12:09:15.16 Loading /home/esp/esp2/mission/GENOMAP.rb mission
@12:09:15.45 <MFBPCR> Extracting Nucleic Acid from extSrc
@12:09:15.50 SPE.hold 2:46:40,55C
@12:09:15.58 ASV.dial :SPEdelivery
@12:09:15.89 <bkha> Session Begun
@12:09:16.08 <MFBPCR> delay 20 seconds
@12:09:28.93 <bkha> -> showlog 100
@12:09:36.11 <MFBPCR> AFV.select :holdingCoil
@12:09:36.23 ATV.dial :air2
@12:09:37.52 AS.reconfigure ASslow1
@12:09:37.58 AS.seek 0.07ml
@12:09:51.97 delay 3 seconds
@12:09:55.00 ATV.dial :extSrc
@12:09:56.50 AS.seek 0.47ml
@12:11:17.27 delay 5 seconds
@12:11:22.35 Solid Phase Extraction Heater at 39C
@12:11:22.36 ATV.dial ASV
@12:11:23.25 ASV.dial :SPEdelivery
@12:11:23.34 AS.seek 0.042ml
@12:12:49.69 delay 10 seconds
@12:12:59.73 Rinsing external lysate line extSrc
@12:12:59.74 AFV.select :carrierSolution
@12:12:59.86 delay 2 seconds
@12:13:01.88 AS.reconfigure ASconfig
@12:13:01.93 AS.seek 1ml
@12:13:57.28 delay 1 second
@12:13:58.30 AFV.select :holdingCoil
@12:13:58.41 delay 1 second
@12:13:59.43 ATV.dial :extSrc
@12:14:00.26 AS.reconfigure ASnorm
@12:14:00.31 AS.seek 0ml
@12:14:50.96 ATV.dial :air2
@12:14:52.56 AS.reconfigure ASslow0
@12:14:52.62 AS.seek 0.05ml
@12:14:58.07 ATV.dial :extSrc
@12:14:59.67 AS.seek 0.01ml
@12:15:04.00 ATV.dial :waste
@12:15:05.39 AS.reconfigure ASconfig
@12:15:05.44 AS.seek 0ml
@12:15:06.66 ethanol wash
@12:15:06.67 ATV.dial :air2
@12:15:07.86 AS.reconfigure ASslow2
@12:15:07.92 AS.seek 0.01ml
@12:15:18.33 delay 3 seconds
@12:15:21.36 Pulling 0.4ml of Ethanol Wash
@12:15:21.37 ATV.dial :ethanol
@12:15:22.25 AS.reconfigure ASslow1
@12:15:22.31 AS.seek 0.41ml
@12:16:43.07 delay 3 seconds
@12:16:46.10 ATV.dial :air1
@12:16:46.52 delay 3 seconds
@12:16:49.59 AS.seek 0.435ml
@12:16:54.97 delay 3 seconds
@12:16:58.00 ATV.dial :ethanol
@12:16:58.63 AS.seek 0.42ml
@12:17:01.96 delay 3 seconds
@12:17:04.99 ATV.dial ASV
@12:17:06.39 AS.seek 0.093ml
@12:18:12.41 delay 3 seconds
@12:18:15.44 ATV.dial :waste2
@12:18:16.26 AS.reconfigure ASconfig
@12:18:16.31 AS.seek 0ml
@12:18:21.96 ATV.dial :air2
@12:18:22.39 AS.reconfigure ASslow1
@12:18:22.44 AS.seek 1ml
@12:21:43.82 delay 5 seconds
@12:21:48.85 ATV.dial ASV
@12:21:49.91 ASV.dial :SPEdelivery
@12:21:49.93 AS.seek 0.02ml
@12:25:07.32 delay 5 seconds
@12:25:12.35 ATV.dial :air2
@12:25:13.45 AS.seek 0.52ml
@12:26:54.21 delay 5 seconds
@12:26:59.24 ATV.dial ASV
@12:27:00.28 ASV.dial :wasteR
@12:27:00.75 AS.seek 0.3ml
@12:27:45.27 delay 20 seconds
@12:28:05.31 AS.seek 0.07ml
@12:28:51.87 delay 20 seconds
@12:29:11.91 ATV.dial :waste2
@12:29:12.72 AS.reconfigure ASconfig
@12:29:12.77 AS.seek 0ml
@12:29:17.08 Elution
@12:29:17.09 ATV.dial :air1
@12:29:18.30 AS.reconfigure ASslow1
@12:29:18.35 AS.seek 0.05ml
@12:29:28.77 delay 3 seconds
@12:29:31.80 ATV.dial :ElutionWater
@12:29:32.52 AS.seek 0.11ml
@12:29:44.88 delay 3 seconds
@12:29:47.91 ATV.dial ASV,via: :air2
@12:29:50.14 ASV.dial :SPEelution
@12:29:50.75 AFV.select :carrierSolution
@12:29:50.91 AS.reconfigure ASnorm
@12:29:50.97 AS.seek 0.76ml
@12:30:24.02 delay 3 seconds
@12:30:27.04 AFV.select :holdingCoil
@12:30:27.19 AS.reconfigure ASslow1
@12:30:27.25 AS.seek 0.475ml
@12:31:24.86 delay 5 seconds
@12:31:29.94 AS.reconfigure ASslow2
@12:31:30.00 AS.seek 0.44ml
@12:32:05.52 delay 1:00
@12:33:05.56 pushing eluate to holding coil
@12:33:05.63 AS.seek 0.28ml
@12:35:46.91 delay 30 seconds
@12:36:16.95 ATV.dial :waste,via: :air3
@12:36:18.79 AS.reconfigure ASconfig
@12:36:18.84 AS.seek 0ml
@12:36:35.25 Parking Bleach in LysateSource line and SPEcolumn
@12:36:35.26 ATV.dial :air3
@12:36:36.16 AS.reconfigure ASslow1
@12:36:36.21 AS.seek 0.03ml
@12:36:42.53 ATV.dial :ElutionWater
@12:36:43.17 AS.seek 0.06ml
@12:36:49.47 ATV.dial :air3
@12:36:50.11 AS.seek 0.07ml
@12:36:52.44 delay 2 seconds
@12:36:54.47 ATV.dial :bleach
@12:36:54.95 AS.seek 0.15ml
@12:37:11.32 delay 3 seconds
@12:37:14.35 ATV.dial :air3
@12:37:14.94 delay 3 seconds
@12:37:18.01 AS.seek 0.175ml
@12:37:23.36 delay 3 seconds
@12:37:26.39 ATV.dial :bleach
@12:37:27.02 AS.seek 0.16ml
@12:37:30.33 delay 3 seconds
@12:37:33.36 ATV.dial :extSrc
@12:37:34.03 AS.reconfigure ASconfig
@12:37:34.08 AS.seek 0.015ml
@12:37:42.71 ATV.dial :waste
@12:37:44.28 AS.seek 0ml
@12:37:45.43 ATV.dial :air3
@12:37:46.33 AS.reconfigure ASslow1
@12:37:46.38 AS.seek 0.01ml
@12:37:48.68 delay 2 seconds
@12:37:50.71 ATV.dial :bleach
@12:37:51.19 AS.seek 0.58ml
@12:39:46.16 delay 3 seconds
@12:39:49.19 ATV.dial :air3
@12:39:49.78 delay 3 seconds
@12:39:52.85 AS.seek 0.605ml
@12:39:58.22 delay 3 seconds
@12:40:01.25 ATV.dial :bleach
@12:40:01.88 AS.seek 0.59ml
@12:40:05.21 delay 3 seconds
@12:40:08.24 ATV.dial :ASV
@12:40:09.35 ASV.dial :wasteR
@12:40:09.98 AS.seek 0ml
@12:42:08.89 delay 1:00
@12:43:08.92 AFV.select :carrierSolution
@12:43:09.03 delay 2 seconds
@12:43:11.05 AS.reconfigure ASconfig
@12:43:11.10 AS.seek 1ml
@12:44:08.86 delay 1 second
@12:44:09.88 AFV.select :holdingCoil
@12:44:09.99 delay 1 second
@12:44:11.01 ATV.dial ASV
@12:44:11.02 ASV.dial :wasteR
@12:44:11.04 AS.reconfigure ASslow1
@12:44:11.10 AS.seek 0ml
@12:47:32.49 delay 20 seconds
@12:47:52.53 ATV.dial :air3
@12:47:54.13 AS.seek 0.59ml
@12:49:53.08 delay 3 seconds
@12:49:56.11 ATV.dial :ASV
@12:49:57.63 ASV.dial :wasteR
@12:49:57.72 AS.seek 0.09ml
@12:51:38.57 delay 30 seconds
@12:52:08.61 ATV.dial :extSrc
@12:52:09.44 delay 3 seconds
@12:52:12.47 ATV.dial :ElutionWater
@12:52:13.68 AS.seek 0.12ml
@12:52:20.00 ATV.dial :air3
@12:52:20.64 AS.seek 0.13ml
@12:52:22.93 delay 2 seconds
@12:52:24.96 ATV.dial :bleach
@12:52:25.44 AS.seek 0.23ml
@12:52:45.86 delay 3 seconds
@12:52:48.89 ATV.dial :air3
@12:52:49.47 delay 3 seconds
@12:52:52.54 AS.seek 0.255ml
@12:52:57.97 delay 3 seconds
@12:53:01.00 ATV.dial :bleach
@12:53:01.64 AS.seek 0.24ml
@12:53:05.36 delay 3 seconds
@12:53:08.39 ATV.dial :ASV
@12:53:09.49 ASV.dial :SPEdelivery
@12:53:09.89 AFV.select :carrierSolution
@12:53:10.01 delay 2 seconds
@12:53:12.03 AS.reconfigure ASconfig
@12:53:12.08 AS.seek 0.5ml
@12:53:27.32 delay 1 second
@12:53:28.34 AFV.select :holdingCoil
@12:53:28.49 AS.reconfigure ASslow1
@12:53:28.55 AS.seek 0.17ml
@12:54:35.23 SPE.hold 2:46:40,95C
@12:54:35.29 delay 3:00
@12:57:35.33 AFV.select :carrierSolution
@12:57:35.45 delay 2 seconds
@12:57:37.47 AS.reconfigure ASconfig
@12:57:37.52 AS.seek 1ml
@12:58:25.52 delay 1 second
@12:58:26.54 AFV.select :holdingCoil
@12:58:26.65 delay 1 second
@12:58:27.67 ASV.dial :wasteD
@12:58:28.78 AS.seek 0ml
@12:59:26.57 Priming Eluate
@12:59:26.58 AFV.select :carrierSolution
@12:59:26.71 delay 2 seconds
@12:59:28.73 AS.seek 0.5ml
@12:59:57.75 delay 1 second
@12:59:58.77 AFV.select :holdingCoil
@12:59:58.88 ATV.dial ASV
@12:59:58.89 ASV.dial :SPErecovery
@12:59:59.52 Swish #1 of 2
@12:59:59.61 AS.reconfigure ASslow0
@12:59:59.66 AS.seek 0.6ml
@13:00:10.13 AS.seek 0.5ml
@13:00:20.55 Swish #2 of 2
@13:00:20.63 AS.seek 0.6ml
@13:00:31.13 AS.seek 0.5ml
@13:00:41.52 delay 3 seconds
@13:00:44.59 AS.reconfigure ASslow1
@13:00:44.65 AS.seek 0.684ml
@13:01:21.94 delay 3 seconds
@13:01:24.97 Eluate primed
@13:01:24.98 ATV.dial :waste2
@13:01:25.65 AS.reconfigure ASnorm
@13:01:25.70 AS.seek 0ml
@13:02:00.50 SPE.coast
@13:02:00.58 ASV.dial :SPErecovery
@13:02:00.60 Setting up PCR for ACYPP2 -- writing /var/log/esp/ACYPP221mar2413h.pcr
@13:02:00.63 Building PCR Slug from ASV with MM1 mix, PP2 primer
@13:02:00.65 Priming with carrier
@13:02:00.66 ATV.dial ARV
@13:02:01.71 ARV.dial :carrier
@13:02:02.06 AS.seek 0.5ml
@13:02:27.61 AFV.select :carrierSolution
@13:02:27.74 delay 2 seconds
@13:02:29.76 AS.reconfigure ASconfig
@13:02:29.83 AS.seek 1ml
@13:02:58.85 delay 1 second
@13:02:59.87 AFV.select :holdingCoil
@13:02:59.98 delay 1 second
@13:03:01.00 ATV.dial :waste
@13:03:02.62 AS.reconfigure ASnorm
@13:03:02.68 AS.seek 0ml
@13:03:53.38 AFV.select :carrierSolution
@13:03:53.50 delay 2 seconds
@13:03:55.52 AS.reconfigure ASconfig
@13:03:55.58 AS.seek 0.5ml
@13:04:24.64 delay 1 second
@13:04:25.66 AFV.select :holdingCoil
@13:04:25.77 Building Slug
@13:04:25.78 ATV.dial :ARV
@13:04:27.55 delay 2 seconds
@13:04:29.58 ARV.dial :air1
@13:04:29.98 delay 2 seconds
@13:04:32.05 AS.reconfigure ASslow2
@13:04:32.11 AS.seek 0.506ml
@13:04:38.68 delay 2 seconds
@13:04:40.71 ARV.dial :MM1
@13:04:41.35 delay 2 seconds
@13:04:43.38 Adding MM1
@13:04:43.45 AS.seek 0.524ml
@13:05:01.93 delay 25 seconds
@13:05:26.96 ARV.dial :PP2
@13:05:27.39 Adding PP2
@13:05:27.40 delay 2 seconds
@13:05:29.48 AS.seek 0.53ml
@13:05:35.69 delay 10 seconds
@13:05:45.73 ARV.dial :air2
@13:05:46.14 adding ARV air
@13:05:46.15 delay 2 seconds
@13:05:48.23 AS.seek 0.536ml
@13:05:54.64 delay 5 seconds
@13:05:59.67 ARV.dial :carrier
@13:06:00.99 delay 7 seconds
@13:06:08.07 AS.seek 0.594ml
@13:07:06.50 delay 5 seconds
@13:07:11.53 ATV.dial :ASV
@13:07:11.94 delay 2 seconds
@13:07:14.02 AS.seek 0.6ml
@13:07:20.44 delay 4 seconds
@13:07:24.47 ATV.dial ARV
@13:07:25.05 delay 2 seconds
@13:07:27.08 ARV.dial :carrier
@13:07:27.09 delay 2 seconds
@13:07:29.17 AS.seek 0.65ml
@13:08:19.75 delay 4 seconds
@13:08:23.78 ATV.dial PCR
@13:08:24.43 ASV.dial :waste1
@13:08:25.24 ARV.dial :air1
@13:08:25.65 Positioning Slug by 0.324ml
@13:08:25.73 AS.seek 0.326ml
@13:13:51.93 delay 5 seconds
@13:13:56.96 ATV.dial ARV
@13:13:57.77 ASV.dial :PCRlock
@13:13:58.16 delay 5 seconds
@13:14:03.19 Running ACYPP2 Profile60CIQ2 thermocycling
@13:14:03.20 <PCR> PCR.program Profile60CIQ2
@13:14:03.33 Profile60CIQ2 should complete in < 1:47:50
@13:14:03.34 PCR.run 2:19:47.5
@14:42:28.82 PCR.off
@14:42:29.44 <MFBPCR> Running ACYPP2 MeltProfile60 thermocycling
@14:42:29.83 <PCR> PCR.program MeltProfile60
@14:42:29.93 MeltProfile60 should complete in < 11:55
@14:42:29.94 PCR.run 19:53.75
@14:50:04.52 PCR.off
@14:50:05.45 <MFBPCR> PCR for ACYPP2 completed -- closed /var/log/esp/ACYPP221mar2413h.pcr
@14:50:05.52 Cleaning PCR
@14:50:05.53 AFV.select :carrierSolution
@14:50:05.66 delay 2 seconds
@14:50:07.68 AS.reconfigure ASconfig
@14:50:07.74 AS.seek 0.5ml
@14:50:18.05 delay 1 second
@14:50:19.07 AFV.select :holdingCoil
@14:50:19.18 ASV.dial :waste1
@14:50:19.57 ATV.dial :air3
@14:50:20.94 AS.reconfigure ASslow1
@14:50:21.00 AS.seek 0.51ml
@14:50:23.30 delay 2 seconds
@14:50:25.32 ATV.dial :bleach
@14:50:25.97 AS.seek 0.81ml
@14:51:26.55 delay 3 seconds
@14:51:29.58 ATV.dial :air3
@14:51:30.16 delay 3 seconds
@14:51:33.23 AS.seek 0.835ml
@14:51:38.55 delay 3 seconds
@14:51:41.58 ATV.dial :bleach
@14:51:42.22 AS.seek 0.82ml
@14:51:45.52 delay 3 seconds
@14:51:48.55 ARV.dial :waste
@14:51:49.18 delay 3 seconds
@14:51:52.21 ATV.dial :ARV
@14:51:53.13 AS.reconfigure ASconfig
@14:51:53.19 AS.seek 0.47ml
@14:52:13.61 AFV.select :carrierSolution
@14:52:13.72 delay 2 seconds
@14:52:15.74 AS.seek 1ml
@14:52:46.49 delay 1 second
@14:52:47.51 AFV.select :holdingCoil
@14:52:47.62 delay 1 second
@14:52:48.64 AS.reconfigure ASfast
@14:52:48.69 AS.seek 0ml
@14:53:16.04 AFV.select :carrierSolution
@14:53:16.16 delay 2 seconds
@14:53:18.18 AS.reconfigure ASconfig
@14:53:18.23 AS.seek 1ml
@14:54:15.99 delay 1 second
@14:54:17.01 AFV.select :holdingCoil
@14:54:17.12 delay 1 second
@14:54:18.14 AS.reconfigure ASfast
@14:54:18.20 AS.seek 0ml
@14:54:45.55 AFV.select :carrierSolution
@14:54:45.67 delay 2 seconds
@14:54:47.69 AS.reconfigure ASconfig
@14:54:47.74 AS.seek 1ml
@14:55:45.50 delay 1 second
@14:55:46.52 AFV.select :holdingCoil
@14:55:46.63 delay 1 second
@14:55:47.65 AS.reconfigure ASfast
@14:55:47.71 AS.seek 0ml
@14:56:15.07 ARV.dial between carrier and air1
@14:56:15.61 ATV.dial :air3
@14:56:16.97 AS.reconfigure ASslow1
@14:56:17.03 AS.seek 0.01ml
@14:56:19.44 delay 2 seconds
@14:56:21.47 ATV.dial :bleach
@14:56:22.13 AS.seek 0.81ml
@14:59:03.29 delay 3 seconds
@14:59:06.32 ATV.dial :air3
@14:59:06.89 delay 3 seconds
@14:59:09.96 AS.seek 0.835ml
@14:59:15.25 delay 3 seconds
@14:59:18.28 ATV.dial :bleach
@14:59:18.92 AS.seek 0.82ml
@14:59:22.21 delay 3 seconds
@14:59:25.24 ATV.dial PCR
@14:59:25.67 AS.reconfigure ASconfig
@14:59:25.72 AS.seek 0ml
@15:00:13.13 PCR.program :bleachProfile
@15:00:13.20 PCR program should complete in < 2:45
@15:00:13.21 PCR.run 10:00
@15:03:09.42 PCR.off
@15:03:09.46 AFV.select :carrierSolution
@15:03:09.57 delay 2 seconds
@15:03:11.59 AS.seek 1ml
@15:04:09.35 delay 1 second
@15:04:10.37 AFV.select :holdingCoil
@15:04:10.48 delay 1 second
@15:04:11.50 AS.reconfigure ASfast
@15:04:11.56 AS.seek 0ml
@15:04:38.91 delay 3 seconds
@15:04:41.93 AFV.select :carrierSolution
@15:04:42.04 delay 2 seconds
@15:04:44.06 AS.reconfigure ASconfig
@15:04:44.11 AS.seek 1ml
@15:05:41.86 delay 1 second
@15:05:42.88 AFV.select :holdingCoil
@15:05:42.99 delay 1 second
@15:05:44.01 AS.reconfigure ASfast
@15:05:44.07 AS.seek 0ml
@15:06:11.43 delay 3 seconds
@15:06:14.45 AFV.select :carrierSolution
@15:06:14.56 delay 2 seconds
@15:06:16.58 AS.reconfigure ASconfig
@15:06:16.64 AS.seek 1ml
@15:07:14.43 delay 1 second
@15:07:15.45 AFV.select :holdingCoil
@15:07:15.56 delay 1 second
@15:07:16.58 AS.reconfigure ASfast
@15:07:16.64 AS.seek 0ml
@15:07:43.99 delay 3 seconds
@15:07:47.01 AFV.select :carrierSolution
@15:07:47.12 delay 2 seconds
@15:07:49.14 AS.reconfigure ASconfig
@15:07:49.19 AS.seek 1ml
@15:08:46.93 delay 1 second
@15:08:47.95 AFV.select :holdingCoil
@15:08:48.06 delay 1 second
@15:08:49.08 AS.reconfigure ASfast
@15:08:49.13 AS.seek 0ml
@15:09:16.47 delay 3 seconds
@15:09:19.49 AFV.select :carrierSolution
@15:09:19.60 delay 2 seconds
@15:09:21.62 AS.reconfigure ASconfig
@15:09:21.69 AS.seek 1ml
@15:10:19.44 delay 1 second
@15:10:20.46 AFV.select :holdingCoil
@15:10:20.57 delay 1 second
@15:10:21.59 AS.reconfigure ASnorm
@15:10:21.64 AS.seek 0ml
@15:11:12.35 Setting up PCR for AOPP1 -- writing /var/log/esp/AOPP121mar2415h.pcr
@15:11:12.36 Building PCR Slug from ASV with MM1 mix, PP1 primer
@15:11:12.39 Priming with carrier
@15:11:12.40 ATV.dial ARV
@15:11:13.05 ARV.dial :carrier
@15:11:13.41 AS.seek 0.5ml
@15:11:39.04 AFV.select :carrierSolution
@15:11:39.16 delay 2 seconds
@15:11:41.18 AS.reconfigure ASconfig
@15:11:41.24 AS.seek 1ml
@15:12:10.22 delay 1 second
@15:12:11.24 AFV.select :holdingCoil
@15:12:11.35 delay 1 second
@15:12:12.37 ATV.dial :waste
@15:12:14.16 AS.reconfigure ASnorm
@15:12:14.22 AS.seek 0ml
@15:13:04.91 AFV.select :carrierSolution
@15:13:05.04 delay 2 seconds
@15:13:07.06 AS.reconfigure ASconfig
@15:13:07.12 AS.seek 0.5ml
@15:13:36.18 delay 1 second
@15:13:37.20 AFV.select :holdingCoil
@15:13:37.31 Building Slug
@15:13:37.32 ATV.dial :ARV
@15:13:39.08 delay 2 seconds
@15:13:41.11 ARV.dial :air1
@15:13:41.51 delay 2 seconds
@15:13:43.58 AS.reconfigure ASslow2
@15:13:43.64 AS.seek 0.506ml
@15:13:50.05 delay 2 seconds
@15:13:52.08 ARV.dial :MM1
@15:13:52.71 delay 2 seconds
@15:13:54.74 Adding MM1
@15:13:54.81 AS.seek 0.524ml
@15:14:13.36 delay 25 seconds
@15:14:38.39 ARV.dial :PP1
@15:14:38.80 Adding PP1
@15:14:38.81 delay 2 seconds
@15:14:40.88 AS.seek 0.53ml
@15:14:47.19 delay 10 seconds
@15:14:57.23 ARV.dial :air1
@15:14:57.66 adding ARV air
@15:14:57.67 delay 2 seconds
@15:14:59.74 AS.seek 0.536ml
@15:15:06.11 delay 5 seconds
@15:15:11.14 ARV.dial :carrier
@15:15:11.55 delay 7 seconds
@15:15:18.64 AS.seek 0.594ml
@15:16:17.11 delay 5 seconds
@15:16:22.14 ATV.dial :ASV
@15:16:22.55 delay 2 seconds
@15:16:24.63 AS.seek 0.6ml
@15:16:30.86 delay 4 seconds
@15:16:34.89 ATV.dial ARV
@15:16:35.47 delay 2 seconds
@15:16:37.50 ARV.dial :carrier
@15:16:37.51 delay 2 seconds
@15:16:39.58 AS.seek 0.65ml
@15:17:30.18 delay 4 seconds
@15:17:34.21 ATV.dial PCR
@15:17:34.85 ASV.dial :waste1
@15:17:34.87 ARV.dial :air1
@15:17:35.29 Positioning Slug by 0.324ml
@15:17:35.37 AS.seek 0.326ml
@15:23:01.33 delay 5 seconds
@15:23:06.36 ATV.dial ARV
@15:23:07.29 ASV.dial :PCRlock
@15:23:08.13 delay 5 seconds
@15:23:13.16 Running AOPP1 Profile60CIQ1 thermocycling
@15:23:13.17 <PCR> PCR.program Profile60CIQ1
@15:23:13.29 Profile60CIQ1 should complete in < 1:56:15
@15:23:13.30 PCR.run 2:30:18.75
@17:01:59.58 PCR.off
@17:02:00.14 <MFBPCR> Running AOPP1 MeltProfile60 thermocycling
@17:02:00.16 <PCR> PCR.program MeltProfile60
@17:02:00.26 MeltProfile60 should complete in < 11:55
@17:02:00.27 PCR.run 19:53.75
@17:09:28.54 PCR.off
@17:09:29.49 <MFBPCR> PCR for AOPP1 completed -- closed /var/log/esp/AOPP121mar2415h.pcr
@17:09:29.56 Cleaning PCR
@17:09:29.57 AFV.select :carrierSolution
@17:09:29.69 delay 2 seconds
@17:09:31.71 AS.reconfigure ASconfig
@17:09:31.76 AS.seek 0.5ml
@17:09:42.05 delay 1 second
@17:09:43.07 AFV.select :holdingCoil
@17:09:43.18 ASV.dial :waste1
@17:09:43.57 ATV.dial :air3
@17:09:44.92 AS.reconfigure ASslow1
@17:09:44.98 AS.seek 0.51ml
@17:09:47.34 delay 2 seconds
@17:09:49.37 ATV.dial :bleach
@17:09:50.02 AS.seek 0.81ml
@17:10:50.63 delay 3 seconds
@17:10:53.66 ATV.dial :air3
@17:10:54.24 delay 3 seconds
@17:10:57.31 AS.seek 0.835ml
@17:11:02.61 delay 3 seconds
@17:11:05.64 ATV.dial :bleach
@17:11:06.28 AS.seek 0.82ml
@17:11:09.61 delay 3 seconds
@17:11:12.64 ARV.dial :waste
@17:11:13.27 delay 3 seconds
@17:11:16.30 ATV.dial :ARV
@17:11:17.22 AS.reconfigure ASconfig
@17:11:17.27 AS.seek 0.47ml
@17:11:37.69 AFV.select :carrierSolution
@17:11:37.81 delay 2 seconds
@17:11:39.83 AS.seek 1ml
@17:12:10.60 delay 1 second
@17:12:11.62 AFV.select :holdingCoil
@17:12:11.73 delay 1 second
@17:12:12.75 AS.reconfigure ASfast
@17:12:12.80 AS.seek 0ml
@17:12:40.15 AFV.select :carrierSolution
@17:12:40.27 delay 2 seconds
@17:12:42.29 AS.reconfigure ASconfig
@17:12:42.34 AS.seek 1ml
@17:13:40.13 delay 1 second
@17:13:41.15 AFV.select :holdingCoil
@17:13:41.26 delay 1 second
@17:13:42.28 AS.reconfigure ASfast
@17:13:42.34 AS.seek 0ml
@17:14:09.69 AFV.select :carrierSolution
@17:14:09.81 delay 2 seconds
@17:14:11.83 AS.reconfigure ASconfig
@17:14:11.88 AS.seek 1ml
@17:15:09.61 delay 1 second
@17:15:10.63 AFV.select :holdingCoil
@17:15:10.74 delay 1 second
@17:15:11.76 AS.reconfigure ASfast
@17:15:11.82 AS.seek 0ml
@17:15:39.15 ARV.dial between carrier and air1
@17:15:39.69 ATV.dial :air3
@17:15:41.05 AS.reconfigure ASslow1
@17:15:41.11 AS.seek 0.01ml
@17:15:43.51 delay 2 seconds
@17:15:45.54 ATV.dial :bleach
@17:15:46.19 AS.seek 0.81ml
@17:18:27.46 delay 3 seconds
@17:18:30.55 ATV.dial :air3
@17:18:31.34 delay 3 seconds
@17:18:34.65 AS.seek 0.835ml
@17:18:40.63 delay 3 seconds
@17:18:43.65 ATV.dial :bleach
@17:18:44.29 AS.seek 0.82ml
@17:18:47.61 delay 3 seconds
@17:18:50.66 ATV.dial PCR
@17:18:51.08 AS.reconfigure ASconfig
@17:18:51.13 AS.seek 0ml
@17:19:38.52 PCR.program :bleachProfile
@17:19:38.60 PCR program should complete in < 2:45
@17:19:38.61 PCR.run 10:00
@17:22:34.84 PCR.off
@17:22:34.88 AFV.select :carrierSolution
@17:22:34.99 delay 2 seconds
@17:22:37.01 AS.seek 1ml
@17:23:34.76 delay 1 second
@17:23:35.78 AFV.select :holdingCoil
@17:23:35.89 delay 1 second
@17:23:36.91 AS.reconfigure ASfast
@17:23:36.97 AS.seek 0ml
@17:24:04.30 delay 3 seconds
@17:24:07.32 AFV.select :carrierSolution
@17:24:07.43 delay 2 seconds
@17:24:09.45 AS.reconfigure ASconfig
@17:24:09.51 AS.seek 1ml
@17:25:07.30 delay 1 second
@17:25:08.32 AFV.select :holdingCoil
@17:25:08.43 delay 1 second
@17:25:09.45 AS.reconfigure ASfast
@17:25:09.51 AS.seek 0ml
@17:25:36.82 delay 3 seconds
@17:25:39.84 AFV.select :carrierSolution
@17:25:39.95 delay 2 seconds
@17:25:41.97 AS.reconfigure ASconfig
@17:25:42.03 AS.seek 1ml
@17:26:39.82 delay 1 second
@17:26:40.84 AFV.select :holdingCoil
@17:26:40.95 delay 1 second
@17:26:41.97 AS.reconfigure ASfast
@17:26:42.03 AS.seek 0ml
@17:27:09.38 delay 3 seconds
@17:27:12.40 AFV.select :carrierSolution
@17:27:12.51 delay 2 seconds
@17:27:14.53 AS.reconfigure ASconfig
@17:27:14.59 AS.seek 1ml
@17:28:12.35 delay 1 second
@17:28:13.37 AFV.select :holdingCoil
@17:28:13.48 delay 1 second
@17:28:14.50 AS.reconfigure ASfast
@17:28:14.56 AS.seek 0ml
@17:28:41.91 delay 3 seconds
@17:28:44.93 AFV.select :carrierSolution
@17:28:45.04 delay 2 seconds
@17:28:47.06 AS.reconfigure ASconfig
@17:28:47.11 AS.seek 1ml
@17:29:44.83 delay 1 second
@17:29:45.85 AFV.select :holdingCoil
@17:29:45.96 delay 1 second
@17:29:46.98 AS.reconfigure ASnorm
@17:29:47.03 AS.seek 0ml
@17:30:37.80 Setting up PCR for AARPP4 -- writing /var/log/esp/AARPP421mar2417h.pcr
@17:30:37.82 Building PCR Slug from ASV with MM2 mix, PP4 primer
@17:30:37.84 Priming with carrier
@17:30:37.85 ATV.dial ARV
@17:30:38.51 ARV.dial :carrier
@17:30:38.86 AS.seek 0.5ml
@17:31:04.41 AFV.select :carrierSolution
@17:31:04.54 delay 2 seconds
@17:31:06.56 AS.reconfigure ASconfig
@17:31:06.63 AS.seek 1ml
@17:31:35.63 delay 1 second
@17:31:36.65 AFV.select :holdingCoil
@17:31:36.76 delay 1 second
@17:31:37.78 ATV.dial :waste
@17:31:39.58 AS.reconfigure ASnorm
@17:31:39.64 AS.seek 0ml
@17:32:30.35 AFV.select :carrierSolution
@17:32:30.48 delay 2 seconds
@17:32:32.50 AS.reconfigure ASconfig
@17:32:32.56 AS.seek 0.5ml
@17:33:01.60 delay 1 second
@17:33:02.62 AFV.select :holdingCoil
@17:33:02.73 Building Slug
@17:33:02.74 ATV.dial :ARV
@17:33:04.51 delay 2 seconds
@17:33:06.54 ARV.dial :air1
@17:33:06.94 delay 2 seconds
@17:33:09.01 AS.reconfigure ASslow2
@17:33:09.07 AS.seek 0.506ml
@17:33:15.57 delay 2 seconds
@17:33:17.60 ARV.dial :MM2
@17:33:19.16 delay 2 seconds
@17:33:21.19 Adding MM2
@17:33:21.26 AS.seek 0.524ml
@17:33:39.99 delay 25 seconds
@17:34:05.02 ARV.dial :PP4
@17:34:05.44 Adding PP4
@17:34:05.45 delay 2 seconds
@17:34:07.52 AS.seek 0.53ml
@17:34:13.71 delay 10 seconds
@17:34:23.75 ARV.dial :air3
@17:34:24.17 adding ARV air
@17:34:24.18 delay 2 seconds
@17:34:26.25 AS.seek 0.536ml
@17:34:32.43 delay 5 seconds
@17:34:37.46 ARV.dial :carrier
@17:34:38.77 delay 7 seconds
@17:34:45.84 AS.seek 0.594ml
@17:35:44.46 delay 5 seconds
@17:35:49.49 ATV.dial :ASV
@17:35:49.91 delay 2 seconds
@17:35:51.98 AS.seek 0.6ml
@17:35:58.15 delay 4 seconds
@17:36:02.18 ATV.dial ARV
@17:36:02.76 delay 2 seconds
@17:36:04.79 ARV.dial :carrier
@17:36:04.80 delay 2 seconds
@17:36:07.29 AS.seek 0.65ml
@17:36:58.07 delay 4 seconds
@17:37:02.10 ATV.dial PCR
@17:37:02.74 ASV.dial :waste1
@17:37:02.76 ARV.dial :air1
@17:37:03.18 Positioning Slug by 0.324ml
@17:37:03.26 AS.seek 0.326ml
@17:42:29.49 delay 5 seconds
@17:42:34.52 ATV.dial ARV
@17:42:35.33 ASV.dial :PCRlock
@17:42:35.74 delay 5 seconds
@17:42:40.77 Running AARPP4 Profile58CIQ2 thermocycling
@17:42:40.78 <PCR> PCR.program Profile58CIQ2
@17:42:40.90 Profile58CIQ2 should complete in < 1:36:15
@17:42:40.91 PCR.run 2:05:18.75
@19:03:16.68 PCR.off
@19:03:17.63 <MFBPCR> Running AARPP4 MeltProfile58 thermocycling
@19:03:17.64 <PCR> PCR.program MeltProfile58
@19:03:17.75 MeltProfile58 should complete in < 12:33
@19:03:17.76 PCR.run 20:41.25
@19:10:47.38 PCR.off
@19:10:48.36 <MFBPCR> PCR for AARPP4 completed -- closed /var/log/esp/AARPP421mar2417h.pcr
@19:10:48.43 Cleaning PCR
@19:10:48.44 AFV.select :carrierSolution
@19:10:48.57 delay 2 seconds
@19:10:50.59 AS.reconfigure ASconfig
@19:10:50.64 AS.seek 0.5ml
@19:11:00.95 delay 1 second
@19:11:01.97 AFV.select :holdingCoil
@19:11:02.08 ASV.dial :waste1
@19:11:02.48 ATV.dial :air3
@19:11:03.85 AS.reconfigure ASslow1
@19:11:03.91 AS.seek 0.51ml
@19:11:06.29 delay 2 seconds
@19:11:08.32 ATV.dial :bleach
@19:11:08.97 AS.seek 0.81ml
@19:12:09.57 delay 3 seconds
@19:12:12.60 ATV.dial :air3
@19:12:13.18 delay 3 seconds
@19:12:16.25 AS.seek 0.835ml
@19:12:21.57 delay 3 seconds
@19:12:24.60 ATV.dial :bleach
@19:12:25.24 AS.seek 0.82ml
@19:12:28.52 delay 3 seconds
@19:12:31.55 ARV.dial :waste
@19:12:32.18 delay 3 seconds
@19:12:35.21 ATV.dial :ARV
@19:12:36.13 AS.reconfigure ASconfig
@19:12:36.18 AS.seek 0.47ml
@19:12:56.57 AFV.select :carrierSolution
@19:12:56.68 delay 2 seconds
@19:12:58.70 AS.seek 1ml
@19:13:29.45 delay 1 second
@19:13:30.47 AFV.select :holdingCoil
@19:13:30.58 delay 1 second
@19:13:31.60 AS.reconfigure ASfast
@19:13:31.66 AS.seek 0ml
@19:13:58.98 AFV.select :carrierSolution
@19:13:59.10 delay 2 seconds
@19:14:01.12 AS.reconfigure ASconfig
@19:14:01.17 AS.seek 1ml
@19:14:58.93 delay 1 second
@19:14:59.95 AFV.select :holdingCoil
@19:15:00.06 delay 1 second
@19:15:01.08 AS.reconfigure ASfast
@19:15:01.14 AS.seek 0ml
@19:15:28.46 AFV.select :carrierSolution
@19:15:28.58 delay 2 seconds
@19:15:30.60 AS.reconfigure ASconfig
@19:15:30.65 AS.seek 1ml
@19:16:28.41 delay 1 second
@19:16:29.43 AFV.select :holdingCoil
@19:16:29.54 delay 1 second
@19:16:30.56 AS.reconfigure ASfast
@19:16:30.61 AS.seek 0ml
@19:16:57.95 ARV.dial between carrier and air1
@19:16:58.46 ATV.dial :air3
@19:16:59.83 AS.reconfigure ASslow1
@19:16:59.89 AS.seek 0.01ml
@19:17:02.20 delay 2 seconds
@19:17:04.23 ATV.dial :bleach
@19:17:04.88 AS.seek 0.81ml
@19:19:46.12 delay 3 seconds
@19:19:49.15 ATV.dial :air3
@19:19:49.74 delay 3 seconds
@19:19:52.81 AS.seek 0.835ml
@19:19:58.10 delay 3 seconds
@19:20:01.13 ATV.dial :bleach
@19:20:01.79 AS.seek 0.82ml
@19:20:05.12 delay 3 seconds
@19:20:08.15 ATV.dial PCR
@19:20:08.57 AS.reconfigure ASconfig
@19:20:08.62 AS.seek 0ml
@19:20:56.02 PCR.program :bleachProfile
@19:20:56.09 PCR program should complete in < 2:45
@19:20:56.10 PCR.run 10:00
@19:23:52.31 PCR.off
@19:23:52.35 AFV.select :carrierSolution
@19:23:52.46 delay 2 seconds
@19:23:54.48 AS.seek 1ml
@19:24:52.24 delay 1 second
@19:24:53.26 AFV.select :holdingCoil
@19:24:53.37 delay 1 second
@19:24:54.39 AS.reconfigure ASfast
@19:24:54.45 AS.seek 0ml
@19:25:21.81 delay 3 seconds
@19:25:24.84 AFV.select :carrierSolution
@19:25:24.96 delay 2 seconds
@19:25:26.98 AS.reconfigure ASconfig
@19:25:27.05 AS.seek 1ml
@19:26:24.81 delay 1 second
@19:26:25.83 AFV.select :holdingCoil
@19:26:25.94 delay 1 second
@19:26:26.96 AS.reconfigure ASfast
@19:26:27.02 AS.seek 0ml
@19:26:54.35 delay 3 seconds
@19:26:57.37 AFV.select :carrierSolution
@19:26:57.48 delay 2 seconds
@19:26:59.50 AS.reconfigure ASconfig
@19:26:59.56 AS.seek 1ml
@19:27:57.35 delay 1 second
@19:27:58.37 AFV.select :holdingCoil
@19:27:58.48 delay 1 second
@19:27:59.50 AS.reconfigure ASfast
@19:27:59.56 AS.seek 0ml
@19:28:26.90 delay 3 seconds
@19:28:29.92 AFV.select :carrierSolution
@19:28:30.03 delay 2 seconds
@19:28:32.05 AS.reconfigure ASconfig
@19:28:32.10 AS.seek 1ml
@19:29:29.85 delay 1 second
@19:29:30.87 AFV.select :holdingCoil
@19:29:30.98 delay 1 second
@19:29:32.00 AS.reconfigure ASfast
@19:29:32.05 AS.seek 0ml
@19:29:59.38 delay 3 seconds
@19:30:02.40 AFV.select :carrierSolution
@19:30:02.51 delay 2 seconds
@19:30:04.53 AS.reconfigure ASconfig
@19:30:04.59 AS.seek 1ml
@19:31:02.35 delay 1 second
@19:31:03.37 AFV.select :holdingCoil
@19:31:03.48 delay 1 second
@19:31:04.50 AS.reconfigure ASnorm
@19:31:04.55 AS.seek 0ml
@19:31:55.32 Setting up PCR for AALPP3 -- writing /var/log/esp/AALPP321mar2419h.pcr
@19:31:55.33 Building PCR Slug from ASV with MM2 mix, PP3 primer
@19:31:55.36 Priming with carrier
@19:31:55.38 ATV.dial ARV
@19:31:56.04 ARV.dial :carrier
@19:31:56.38 AS.seek 0.5ml
@19:32:21.90 AFV.select :carrierSolution
@19:32:22.02 delay 2 seconds
@19:32:24.04 AS.reconfigure ASconfig
@19:32:24.10 AS.seek 1ml
@19:32:53.12 delay 1 second
@19:32:54.14 AFV.select :holdingCoil
@19:32:54.25 delay 1 second
@19:32:55.27 ATV.dial :waste
@19:32:57.07 AS.reconfigure ASnorm
@19:32:57.13 AS.seek 0ml
@19:33:47.82 AFV.select :carrierSolution
@19:33:47.95 delay 2 seconds
@19:33:49.97 AS.reconfigure ASconfig
@19:33:50.03 AS.seek 0.5ml
@19:34:19.09 delay 1 second
@19:34:20.11 AFV.select :holdingCoil
@19:34:20.22 Building Slug
@19:34:20.23 ATV.dial :ARV
@19:34:21.99 delay 2 seconds
@19:34:24.02 ARV.dial :air1
@19:34:24.43 delay 2 seconds
@19:34:26.50 AS.reconfigure ASslow2
@19:34:26.56 AS.seek 0.506ml
@19:34:32.87 delay 2 seconds
@19:34:34.90 ARV.dial :MM2
@19:34:36.86 delay 2 seconds
@19:34:38.88 Adding MM2
@19:34:38.96 AS.seek 0.524ml
@19:34:57.32 delay 25 seconds
@19:35:22.35 ARV.dial :PP3
@19:35:22.75 Adding PP3
@19:35:22.76 delay 2 seconds
@19:35:24.84 AS.seek 0.53ml
@19:35:31.35 delay 10 seconds
@19:35:41.39 ARV.dial :air2
@19:35:41.82 adding ARV air
@19:35:41.83 delay 2 seconds
@19:35:43.90 AS.seek 0.536ml
@19:35:50.31 delay 5 seconds
@19:35:55.34 ARV.dial :carrier
@19:35:56.67 delay 7 seconds
@19:36:03.75 AS.seek 0.594ml
@19:37:02.43 delay 5 seconds
@19:37:07.46 ATV.dial :ASV
@19:37:07.87 delay 2 seconds
@19:37:09.94 AS.seek 0.6ml
@19:37:16.56 delay 4 seconds
@19:37:20.59 ATV.dial ARV
@19:37:21.18 delay 2 seconds
@19:37:23.21 ARV.dial :carrier
@19:37:23.22 delay 2 seconds
@19:37:25.29 AS.seek 0.65ml
@19:38:15.82 delay 4 seconds
@19:38:19.85 ATV.dial PCR
@19:38:20.49 ASV.dial :waste1
@19:38:20.51 ARV.dial :air1
@19:38:20.93 Positioning Slug by 0.324ml
@19:38:21.02 AS.seek 0.326ml
@19:43:46.98 delay 5 seconds
@19:43:52.01 ATV.dial ARV
@19:43:52.82 ASV.dial :PCRlock
@19:43:53.23 delay 5 seconds
@19:43:58.26 Running AALPP3 Profile58CIQ2 thermocycling
@19:43:58.27 <PCR> PCR.program Profile58CIQ2
@19:43:58.39 Profile58CIQ2 should complete in < 1:36:15
@19:43:58.40 PCR.run 2:05:18.75
@21:04:34.31 PCR.off
@21:04:34.87 <MFBPCR> Running AALPP3 MeltProfile58 thermocycling
@21:04:34.89 <PCR> PCR.program MeltProfile58
@21:04:34.99 MeltProfile58 should complete in < 12:33
@21:04:35.00 PCR.run 20:41.25
@21:12:11.26 PCR.off
@21:12:12.23 <MFBPCR> PCR for AALPP3 completed -- closed /var/log/esp/AALPP321mar2419h.pcr
@21:12:12.30 Cleaning PCR
@21:12:12.31 AFV.select :carrierSolution
@21:12:12.44 delay 2 seconds
@21:12:14.46 AS.reconfigure ASconfig
@21:12:14.51 AS.seek 0.5ml
@21:12:24.79 delay 1 second
@21:12:25.81 AFV.select :holdingCoil
@21:12:25.92 ASV.dial :waste1
@21:12:26.31 ATV.dial :air3
@21:12:27.68 AS.reconfigure ASslow1
@21:12:27.74 AS.seek 0.51ml
@21:12:30.02 delay 2 seconds
@21:12:32.05 ATV.dial :bleach
@21:12:32.71 AS.seek 0.81ml
@21:13:33.35 delay 3 seconds
@21:13:36.38 ATV.dial :air3
@21:13:36.96 delay 3 seconds
@21:13:40.03 AS.seek 0.835ml
@21:13:45.35 delay 3 seconds
@21:13:48.38 ATV.dial :bleach
@21:13:49.02 AS.seek 0.82ml
@21:13:52.34 delay 3 seconds
@21:13:55.37 ARV.dial :waste
@21:13:56.00 delay 3 seconds
@21:13:59.03 ATV.dial :ARV
@21:13:59.96 AS.reconfigure ASconfig
@21:14:00.01 AS.seek 0.47ml
@21:14:20.40 AFV.select :carrierSolution
@21:14:20.52 delay 2 seconds
@21:14:22.54 AS.seek 1ml
@21:14:53.28 delay 1 second
@21:14:54.30 AFV.select :holdingCoil
@21:14:54.41 delay 1 second
@21:14:55.43 AS.reconfigure ASfast
@21:14:55.49 AS.seek 0ml
@21:15:22.82 AFV.select :carrierSolution
@21:15:22.94 delay 2 seconds
@21:15:24.96 AS.reconfigure ASconfig
@21:15:25.01 AS.seek 1ml
@21:16:22.76 delay 1 second
@21:16:23.78 AFV.select :holdingCoil
@21:16:23.89 delay 1 second
@21:16:24.91 AS.reconfigure ASfast
@21:16:24.97 AS.seek 0ml
@21:16:52.31 AFV.select :carrierSolution
@21:16:52.43 delay 2 seconds
@21:16:54.45 AS.reconfigure ASconfig
@21:16:54.50 AS.seek 1ml
@21:17:52.24 delay 1 second
@21:17:53.26 AFV.select :holdingCoil
@21:17:53.37 delay 1 second
@21:17:54.39 AS.reconfigure ASfast
@21:17:54.45 AS.seek 0ml
@21:18:21.79 ARV.dial between carrier and air1
@21:18:22.29 ATV.dial :air3
@21:18:23.66 AS.reconfigure ASslow1
@21:18:24.13 AS.seek 0.01ml
@21:18:26.46 delay 2 seconds
@21:18:28.49 ATV.dial :bleach
@21:18:29.15 AS.seek 0.81ml
@21:21:10.28 delay 3 seconds
@21:21:13.31 ATV.dial :air3
@21:21:13.89 delay 3 seconds
@21:21:16.96 AS.seek 0.835ml
@21:21:22.32 delay 3 seconds
@21:21:25.35 ATV.dial :bleach
@21:21:25.99 AS.seek 0.82ml
@21:21:29.29 delay 3 seconds
@21:21:32.32 ATV.dial PCR
@21:21:32.74 AS.reconfigure ASconfig
@21:21:32.79 AS.seek 0ml
@21:22:20.20 PCR.program :bleachProfile
@21:22:20.27 PCR program should complete in < 2:45
@21:22:20.28 PCR.run 10:00
@21:25:16.07 PCR.off
@21:25:16.11 AFV.select :carrierSolution
@21:25:16.22 delay 2 seconds
@21:25:18.24 AS.seek 1ml
@21:26:16.03 delay 1 second
@21:26:17.05 AFV.select :holdingCoil
@21:26:17.16 delay 1 second
@21:26:18.18 AS.reconfigure ASfast
@21:26:18.24 AS.seek 0ml
@21:26:45.59 delay 3 seconds
@21:26:48.61 AFV.select :carrierSolution
@21:26:48.72 delay 2 seconds
@21:26:50.74 AS.reconfigure ASconfig
@21:26:50.81 AS.seek 1ml
@21:27:48.54 delay 1 second
@21:27:49.56 AFV.select :holdingCoil
@21:27:49.67 delay 1 second
@21:27:50.69 AS.reconfigure ASfast
@21:27:50.75 AS.seek 0ml
@21:28:18.07 delay 3 seconds
@21:28:21.09 AFV.select :carrierSolution
@21:28:21.20 delay 2 seconds
@21:28:23.22 AS.reconfigure ASconfig
@21:28:23.28 AS.seek 1ml
@21:29:21.07 delay 1 second
@21:29:22.09 AFV.select :holdingCoil
@21:29:22.20 delay 1 second
@21:29:23.22 AS.reconfigure ASfast
@21:29:23.28 AS.seek 0ml
@21:29:50.60 delay 3 seconds
@21:29:53.62 AFV.select :carrierSolution
@21:29:53.73 delay 2 seconds
@21:29:55.75 AS.reconfigure ASconfig
@21:29:55.81 AS.seek 1ml
@21:30:53.60 delay 1 second
@21:30:54.62 AFV.select :holdingCoil
@21:30:54.73 delay 1 second
@21:30:55.75 AS.reconfigure ASfast
@21:30:55.80 AS.seek 0ml
@21:31:23.14 delay 3 seconds
@21:31:26.16 AFV.select :carrierSolution
@21:31:26.27 delay 2 seconds
@21:31:28.29 AS.reconfigure ASconfig
@21:31:28.35 AS.seek 1ml
@21:32:26.14 delay 1 second
@21:32:27.16 AFV.select :holdingCoil
@21:32:27.27 delay 1 second
@21:32:28.29 AS.reconfigure ASnorm
@21:32:28.34 AS.seek 0ml
@21:33:19.06 Setting up PCR for ACOPP5 -- writing /var/log/esp/ACOPP521mar2421h.pcr
@21:33:19.48 Building PCR Slug from ASV with MM3 mix, PP5 primer
@21:33:19.51 Priming with carrier
@21:33:19.52 ATV.dial ARV
@21:33:20.18 ARV.dial :carrier
@21:33:20.52 AS.seek 0.5ml
@21:33:46.09 AFV.select :carrierSolution
@21:33:46.22 delay 2 seconds
@21:33:48.24 AS.reconfigure ASconfig
@21:33:48.31 AS.seek 1ml
@21:34:17.32 delay 1 second
@21:34:18.34 AFV.select :holdingCoil
@21:34:18.45 delay 1 second
@21:34:19.47 ATV.dial :waste
@21:34:21.27 AS.reconfigure ASnorm
@21:34:21.33 AS.seek 0ml
@21:35:12.01 AFV.select :carrierSolution
@21:35:12.13 delay 2 seconds
@21:35:14.15 AS.reconfigure ASconfig
@21:35:14.21 AS.seek 0.5ml
@21:35:43.28 delay 1 second
@21:35:44.30 AFV.select :holdingCoil
@21:35:44.41 Building Slug
@21:35:44.42 ATV.dial :ARV
@21:35:46.18 delay 2 seconds
@21:35:48.21 ARV.dial :air1
@21:35:48.61 delay 2 seconds
@21:35:50.68 AS.reconfigure ASslow2
@21:35:50.74 AS.seek 0.506ml
@21:35:57.06 delay 2 seconds
@21:35:59.09 ARV.dial :MM3
@21:36:00.18 delay 2 seconds
@21:36:02.21 Adding MM3
@21:36:02.29 AS.seek 0.524ml
@21:36:20.90 delay 25 seconds
@21:36:45.93 ARV.dial :PP5
@21:36:46.32 Adding PP5
@21:36:46.34 delay 2 seconds
@21:36:48.41 AS.seek 0.53ml
@21:36:54.81 delay 10 seconds
@21:37:04.85 ARV.dial :air3
@21:37:05.26 adding ARV air
@21:37:05.27 delay 2 seconds
@21:37:07.34 AS.seek 0.536ml
@21:37:13.85 delay 5 seconds
@21:37:18.88 ARV.dial :carrier
@21:37:20.18 delay 7 seconds
@21:37:27.26 AS.seek 0.594ml
@21:38:26.00 delay 5 seconds
@21:38:31.03 ATV.dial :ASV
@21:38:31.43 delay 2 seconds
@21:38:33.50 AS.seek 0.6ml
@21:38:40.07 delay 4 seconds
@21:38:44.10 ATV.dial ARV
@21:38:44.76 delay 2 seconds
@21:38:46.79 ARV.dial :carrier
@21:38:46.80 delay 2 seconds
@21:38:48.87 AS.seek 0.65ml
@21:39:39.62 delay 4 seconds
@21:39:43.65 ATV.dial PCR
@21:39:44.28 ASV.dial :waste1
@21:39:44.30 ARV.dial :air1
@21:39:44.72 Positioning Slug by 0.324ml
@21:39:44.81 AS.seek 0.326ml
@21:45:10.92 delay 5 seconds
@21:45:15.95 ATV.dial ARV
@21:45:16.76 ASV.dial :PCRlock
@21:45:17.17 delay 5 seconds
@21:45:22.20 Running ACOPP5 ProfileIPc thermocycling
@21:45:22.21 <PCR> PCR.program ProfileIPc
@21:45:22.33 ProfileIPc should complete in < 1:26:35
@21:45:22.34 PCR.run 1:53:13.75
@22:53:44.89 PCR.off
@22:53:45.45 <MFBPCR> PCR for ACOPP5 completed -- closed /var/log/esp/ACOPP521mar2421h.pcr
@22:53:45.52 Cleaning PCR
@22:53:45.53 AFV.select :carrierSolution
@22:53:45.66 delay 2 seconds
@22:53:47.68 AS.reconfigure ASconfig
@22:53:47.73 AS.seek 0.5ml
@22:53:58.03 delay 1 second
@22:53:59.05 AFV.select :holdingCoil
@22:53:59.16 ASV.dial :waste1
@22:53:59.56 ATV.dial :air3
@22:54:00.92 AS.reconfigure ASslow1
@22:54:00.98 AS.seek 0.51ml
@22:54:03.26 delay 2 seconds
@22:54:05.29 ATV.dial :bleach
@22:54:05.93 AS.seek 0.81ml
@22:55:06.62 delay 3 seconds
@22:55:09.65 ATV.dial :air3
@22:55:10.23 delay 3 seconds
@22:55:13.30 AS.seek 0.835ml
@22:55:18.62 delay 3 seconds
@22:55:21.65 ATV.dial :bleach
@22:55:22.29 AS.seek 0.82ml
@22:55:25.64 delay 3 seconds
@22:55:28.67 ARV.dial :waste
@22:55:29.29 delay 3 seconds
@22:55:32.32 ATV.dial :ARV
@22:55:33.24 AS.reconfigure ASconfig
@22:55:33.29 AS.seek 0.47ml
@22:55:53.68 AFV.select :carrierSolution
@22:55:53.80 delay 2 seconds
@22:55:55.82 AS.seek 1ml
@22:56:26.56 delay 1 second
@22:56:27.58 AFV.select :holdingCoil
@22:56:27.69 delay 1 second
@22:56:28.71 AS.reconfigure ASfast
@22:56:28.77 AS.seek 0ml
@22:56:56.12 AFV.select :carrierSolution
@22:56:56.24 delay 2 seconds
@22:56:58.26 AS.reconfigure ASconfig
@22:56:58.31 AS.seek 1ml
@22:57:56.04 delay 1 second
@22:57:57.06 AFV.select :holdingCoil
@22:57:57.17 delay 1 second
@22:57:58.19 AS.reconfigure ASfast
@22:57:58.25 AS.seek 0ml
@22:58:25.58 AFV.select :carrierSolution
@22:58:25.70 delay 2 seconds
@22:58:27.72 AS.reconfigure ASconfig
@22:58:27.77 AS.seek 1ml
@22:59:25.53 delay 1 second
@22:59:26.55 AFV.select :holdingCoil
@22:59:26.66 delay 1 second
@22:59:27.68 AS.reconfigure ASfast
@22:59:27.74 AS.seek 0ml
@22:59:55.08 ARV.dial between carrier and air1
@22:59:56.01 ATV.dial :air3
@22:59:57.37 AS.reconfigure ASslow1
@22:59:57.43 AS.seek 0.01ml
@22:59:59.78 delay 2 seconds
@23:00:01.81 ATV.dial :bleach
@23:00:02.46 AS.seek 0.81ml
@23:02:43.65 delay 3 seconds
@23:02:46.68 ATV.dial :air3
@23:02:47.28 delay 3 seconds
@23:02:50.35 AS.seek 0.835ml
@23:02:55.69 delay 3 seconds
@23:02:58.72 ATV.dial :bleach
@23:02:59.37 AS.seek 0.82ml
@23:03:02.68 delay 3 seconds
@23:03:05.71 ATV.dial PCR
@23:03:06.15 AS.reconfigure ASconfig
@23:03:06.20 AS.seek 0ml
@23:03:53.62 PCR.program :bleachProfile
@23:03:53.69 PCR program should complete in < 2:45
@23:03:53.70 PCR.run 10:00
@23:06:49.51 PCR.off
@23:06:49.55 AFV.select :carrierSolution
@23:06:49.66 delay 2 seconds
@23:06:51.68 AS.seek 1ml
@23:07:49.43 delay 1 second
@23:07:50.45 AFV.select :holdingCoil
@23:07:50.56 delay 1 second
@23:07:51.58 AS.reconfigure ASfast
@23:07:51.64 AS.seek 0ml
@23:08:18.98 delay 3 seconds
@23:08:22.00 AFV.select :carrierSolution
@23:08:22.11 delay 2 seconds
@23:08:24.13 AS.reconfigure ASconfig
@23:08:24.18 AS.seek 1ml
@23:09:21.92 delay 1 second
@23:09:22.94 AFV.select :holdingCoil
@23:09:23.05 delay 1 second
@23:09:24.07 AS.reconfigure ASfast
@23:09:24.13 AS.seek 0ml
@23:09:51.47 delay 3 seconds
@23:09:54.49 AFV.select :carrierSolution
@23:09:54.60 delay 2 seconds
@23:09:56.62 AS.reconfigure ASconfig
@23:09:56.68 AS.seek 1ml
@23:10:54.47 delay 1 second
@23:10:55.49 AFV.select :holdingCoil
@23:10:55.60 delay 1 second
@23:10:56.62 AS.reconfigure ASfast
@23:10:56.68 AS.seek 0ml
@23:11:24.01 delay 3 seconds
@23:11:27.03 AFV.select :carrierSolution
@23:11:27.14 delay 2 seconds
@23:11:29.16 AS.reconfigure ASconfig
@23:11:29.21 AS.seek 1ml
@23:12:27.00 delay 1 second
@23:12:28.02 AFV.select :holdingCoil
@23:12:28.13 delay 1 second
@23:12:29.15 AS.reconfigure ASfast
@23:12:29.20 AS.seek 0ml
@23:12:56.54 delay 3 seconds
@23:12:59.56 AFV.select :carrierSolution
@23:12:59.67 delay 2 seconds
@23:13:01.69 AS.reconfigure ASconfig
@23:13:01.76 AS.seek 1ml
@23:13:59.51 delay 1 second
@23:14:00.53 AFV.select :holdingCoil
@23:14:01.05 delay 1 second
@23:14:02.07 AS.reconfigure ASnorm
@23:14:02.13 AS.seek 0ml
@23:14:52.86 Setting up PCR for AIPc -- writing /var/log/esp/AIPc21mar2423h.pcr
@23:14:52.87 Building PCR Slug from ASV with MM3 mix, PP6 primer
@23:14:52.90 Priming with carrier
@23:14:52.91 ATV.dial ARV
@23:14:53.56 ARV.dial :carrier
@23:14:53.90 AS.seek 0.5ml
@23:15:19.48 AFV.select :carrierSolution
@23:15:19.61 delay 2 seconds
@23:15:21.63 AS.reconfigure ASconfig
@23:15:21.70 AS.seek 1ml
@23:15:50.75 delay 1 second
@23:15:51.77 AFV.select :holdingCoil
@23:15:51.88 delay 1 second
@23:15:52.90 ATV.dial :waste
@23:15:54.68 AS.reconfigure ASnorm
@23:15:54.74 AS.seek 0ml
@23:16:45.42 AFV.select :carrierSolution
@23:16:45.54 delay 2 seconds
@23:16:47.56 AS.reconfigure ASconfig
@23:16:47.62 AS.seek 0.5ml
@23:17:16.67 delay 1 second
@23:17:17.69 AFV.select :holdingCoil
@23:17:17.80 Building Slug
@23:17:17.81 ATV.dial :ARV
@23:17:19.56 delay 2 seconds
@23:17:21.59 ARV.dial :air1
@23:17:21.99 delay 2 seconds
@23:17:24.06 AS.reconfigure ASslow2
@23:17:24.12 AS.seek 0.506ml
@23:17:30.61 delay 2 seconds
@23:17:32.64 ARV.dial :MM3
@23:17:33.73 delay 2 seconds
@23:17:35.76 Adding MM3
@23:17:35.84 AS.seek 0.524ml
@23:17:54.70 delay 25 seconds
@23:18:19.73 ARV.dial :PP6
@23:18:20.15 Adding PP6
@23:18:20.16 delay 2 seconds
@23:18:22.23 AS.seek 0.53ml
@23:18:28.58 delay 10 seconds
@23:18:38.62 ARV.dial :air1
@23:18:39.47 adding ARV air
@23:18:39.49 delay 2 seconds
@23:18:41.56 AS.seek 0.536ml
@23:18:48.20 delay 5 seconds
@23:18:53.23 ARV.dial :carrier
@23:18:53.64 delay 7 seconds
@23:19:00.71 AS.seek 0.594ml
@23:19:59.54 delay 5 seconds
@23:20:04.57 ATV.dial :ASV
@23:20:05.00 delay 2 seconds
@23:20:07.07 AS.seek 0.6ml
@23:20:13.86 delay 4 seconds
@23:20:17.89 ATV.dial ARV
@23:20:18.47 delay 2 seconds
@23:20:20.50 ARV.dial :carrier
@23:20:20.51 delay 2 seconds
@23:20:22.58 AS.seek 0.65ml
@23:21:13.45 delay 4 seconds
@23:21:17.48 ATV.dial PCR
@23:21:18.12 ASV.dial :waste1
@23:21:18.14 ARV.dial :air1
@23:21:18.55 Positioning Slug by 0.324ml
@23:21:18.64 AS.seek 0.326ml
@23:26:44.83 delay 5 seconds
@23:26:49.86 ATV.dial ARV
@23:26:50.67 ASV.dial :PCRlock
@23:26:51.08 delay 5 seconds
@23:26:56.11 Running AIPc ProfileIPc thermocycling
@23:26:56.12 <PCR> PCR.program ProfileIPc
@23:26:56.24 ProfileIPc should complete in < 1:26:35
@23:26:56.25 PCR.run 1:53:13.75
@00:35:30.37CET25-Mar-21 PCR.off
@00:35:30.93 <MFBPCR> PCR for AIPc completed -- closed /var/log/esp/AIPc21mar2423h.pcr
@00:35:30.95 Full MFB Decontamination
@00:35:30.97 Decon extraction column and decon extSrc
@00:35:30.99 SPE.hold 2:46:40,55C
@00:35:31.05 ASV.dial :SPEdelivery
@00:35:31.72 delay 20 seconds
@00:35:51.75 AFV.select :carrierSolution
@00:35:51.86 delay 2 seconds
@00:35:53.88 AS.reconfigure ASconfig
@00:35:53.94 AS.seek 0.5ml
@00:36:04.25 delay 1 second
@00:36:05.27 AFV.select :holdingCoil
@00:36:05.38 ATV.dial :air1
@00:36:07.65 AS.reconfigure ASslow1
@00:36:07.71 AS.seek 0.52ml
@00:36:11.92 ATV.dial :ASV
@00:36:13.70 ASV.dial :SPErecovery
@00:36:14.40 AS.seek 0.62ml
@00:36:34.81 ATV.dial :waste
@00:36:37.06 AS.reconfigure ASnorm
@00:36:37.12 AS.seek 0ml
@00:37:08.69 deconEluateRecoveryLine extSrc
@00:37:08.70 ATV.dial :waste
@00:37:08.74 AS.seek 0ml
@00:37:08.82 ATV.dial :air3
@00:37:09.57 AS.reconfigure ASslow1
@00:37:09.63 AS.seek 0.01ml
@00:37:11.95 delay 2 seconds
@00:37:13.98 ATV.dial :bleach
@00:37:14.48 AS.seek 0.31ml
@00:38:15.14 delay 3 seconds
@00:38:18.17 ATV.dial :air3
@00:38:18.80 delay 3 seconds
@00:38:21.87 AS.seek 0.335ml
@00:38:27.22 delay 3 seconds
@00:38:30.25 ATV.dial :bleach
@00:38:30.92 AS.seek 0.32ml
@00:38:34.14 delay 3 seconds
@00:38:37.17 ATV.dial :extSrc
@00:38:38.26 AS.seek 0ml
@00:39:42.89 AFV.select :carrierSolution
@00:39:43.01 delay 2 seconds
@00:39:45.03 AS.reconfigure ASconfig
@00:39:45.09 AS.seek 0.5ml
@00:40:14.14 delay 1 second
@00:40:15.16 AFV.select :holdingCoil
@00:40:15.27 ATV.dial :extSrc
@00:40:15.29 AS.reconfigure ASslow1
@00:40:15.36 AS.seek 0ml
@00:41:56.19 AFV.select :carrierSolution
@00:41:56.31 delay 2 seconds
@00:41:58.33 AS.reconfigure ASconfig
@00:41:58.39 AS.seek 0.5ml
@00:42:27.42 delay 1 second
@00:42:28.44 AFV.select :holdingCoil
@00:42:28.55 ATV.dial :extSrc
@00:42:28.57 AS.reconfigure ASslow1
@00:42:28.64 AS.seek 0ml
@00:44:09.45 AFV.select :carrierSolution
@00:44:09.57 delay 2 seconds
@00:44:11.59 AS.reconfigure ASconfig
@00:44:11.65 AS.seek 0.5ml
@00:44:40.67 delay 1 second
@00:44:41.69 AFV.select :holdingCoil
@00:44:41.80 ATV.dial :extSrc
@00:44:41.82 AS.reconfigure ASslow1
@00:44:41.89 AS.seek 0ml
@00:46:22.72 AFV.select :carrierSolution
@00:46:22.84 delay 2 seconds
@00:46:24.86 AS.reconfigure ASconfig
@00:46:24.92 AS.seek 0.5ml
@00:46:53.95 delay 1 second
@00:46:54.97 AFV.select :holdingCoil
@00:46:55.08 ATV.dial :extSrc
@00:46:55.10 AS.reconfigure ASslow1
@00:46:55.16 AS.seek 0ml
@00:48:35.98 ATV.dial :air2
@00:48:37.37 AS.reconfigure ASslow0
@00:48:37.43 AS.seek 0.5ml
@00:49:28.03 ATV.dial :extSrc
@00:49:29.62 AS.seek 0.02ml
@00:50:18.22 delay 1 second
@00:50:19.25 ATV.dial :waste
@00:50:20.62 AS.reconfigure ASconfig
@00:50:20.67 AS.seek 0ml
@00:50:22.14 AFV.select :holdingCoil
@00:50:22.16 ATV.dial :waste
@00:50:22.18 AS.seek 0ml
@00:50:22.26 AFV.select :carrierSolution
@00:50:22.38 delay 2 seconds
@00:50:24.40 AS.seek 0.5ml
@00:50:53.45 delay 1 second
@00:50:54.47 AFV.select :holdingCoil
@00:50:54.58 ATV.dial :air3
@00:50:55.47 AS.reconfigure ASslow1
@00:50:55.53 AS.seek 0.52ml
@00:50:59.83 ATV.dial :air3
@00:50:59.92 AS.seek 0.53ml
@00:51:02.23 delay 2 seconds
@00:51:04.26 ATV.dial :bleach
@00:51:04.73 AS.seek 0.71ml
@00:51:41.23 delay 3 seconds
@00:51:44.26 ATV.dial :air3
@00:51:44.84 delay 3 seconds
@00:51:47.91 AS.seek 0.735ml
@00:51:53.20 delay 3 seconds
@00:51:56.23 ATV.dial :bleach
@00:51:56.87 AS.seek 0.72ml
@00:52:00.28 delay 3 seconds
@00:52:03.31 ATV.dial ASV
@00:52:04.42 ASV.dial :SPErecovery
@00:52:04.51 AS.seek 0.365ml
@00:53:16.12 ATV.dial :waste
@00:53:17.95 AS.reconfigure ASnorm
@00:53:18.01 AS.seek 0ml
@00:53:36.73 ATV.dial :air3
@00:53:37.43 AS.reconfigure ASslow1
@00:53:37.49 AS.seek 0.01ml
@00:53:39.80 delay 2 seconds
@00:53:41.83 ATV.dial :bleach
@00:53:42.31 AS.seek 0.58ml
@00:55:37.20 delay 3 seconds
@00:55:40.23 ATV.dial :air3
@00:55:40.83 delay 3 seconds
@00:55:43.90 AS.seek 0.605ml
@00:55:49.20 delay 3 seconds
@00:55:52.23 ATV.dial :bleach
@00:55:52.87 AS.seek 0.59ml
@00:55:56.19 delay 3 seconds
@00:55:59.22 ATV.dial :ASV
@00:56:00.31 ASV.dial :wasteR
@00:56:00.71 AS.seek 0ml
@00:57:59.62 delay 1:00
@00:58:59.66 ATV.dial ASV
@00:58:59.67 AFV.select :carrierSolution
@00:58:59.79 delay 2 seconds
@00:59:01.81 AS.reconfigure ASconfig
@00:59:01.86 AS.seek 1ml
@00:59:59.61 delay 1 second
@01:00:00.63 AFV.select :holdingCoil
@01:00:00.74 delay 1 second
@01:00:01.76 ASV.dial :wasteR
@01:00:01.77 AS.reconfigure ASslow1
@01:00:01.85 AS.seek 0ml
@01:03:23.23 delay 10 seconds
@01:03:33.27 ATV.dial :air3
@01:03:34.89 AS.seek 0.01ml
@01:03:37.20 delay 2 seconds
@01:03:39.23 ATV.dial :bleach
@01:03:40.32 AS.seek 0.58ml
@01:05:35.20 delay 3 seconds
@01:05:38.23 ATV.dial :air3
@01:05:38.81 delay 3 seconds
@01:05:41.89 AS.seek 0.605ml
@01:05:47.17 delay 3 seconds
@01:05:50.20 ATV.dial :bleach
@01:05:50.84 AS.seek 0.59ml
@01:05:54.17 delay 3 seconds
@01:05:57.20 ATV.dial ASV
@01:05:58.30 ASV.dial :SPEdelivery
@01:05:58.70 AS.seek 0ml
@01:07:57.59 delay 1:00
@01:08:57.62 AFV.select :carrierSolution
@01:08:57.73 delay 2 seconds
@01:08:59.75 AS.reconfigure ASconfig
@01:08:59.81 AS.seek 1ml
@01:09:57.58 delay 1 second
@01:09:58.60 AFV.select :holdingCoil
@01:09:58.71 delay 1 second
@01:09:59.73 ATV.dial ASV
@01:09:59.74 ASV.dial :SPEdelivery
@01:09:59.76 AS.reconfigure ASslow1
@01:09:59.84 AS.seek 0ml
@01:13:21.28 delay 10 seconds
@01:13:31.32 ATV.dial :air2
@01:13:32.21 AS.reconfigure ASslow0
@01:13:32.27 AS.seek 0.8ml
@01:14:53.02 delay 3 seconds
@01:14:56.05 ATV.dial :ASV
@01:14:57.09 ASV.dial :SPEdelivery
@01:14:57.11 AS.reconfigure ASslow1
@01:14:57.18 AS.seek 0.03ml
@01:17:32.31 delay 20 seconds
@01:17:52.35 ATV.dial :waste,via: :air3
@01:17:54.18 AS.reconfigure ASconfig
@01:17:54.23 AS.seek 0ml
@01:17:56.25 SPE.coast
@01:17:56.33 Removing Bleach from Mixing Coil
@01:17:56.34 AFV.select :carrierSolution
@01:17:56.46 delay 2 seconds
@01:17:58.48 AS.seek 0.5ml
@01:18:27.50 delay 1 second
@01:18:28.52 AFV.select :holdingCoil
@01:18:28.63 ATV.dial :ASV
@01:18:30.59 ASV.dial :SPErecovery
@01:18:31.25 AS.reconfigure ASslow1
@01:18:31.31 AS.seek 0.73ml
@01:19:17.84 ASV.dial :wasteD
@01:19:18.45 AS.reconfigure ASnorm
@01:19:18.50 AS.seek 0ml
@01:19:55.62 AFV.select :carrierSolution
@01:19:55.74 delay 2 seconds
@01:19:57.76 AS.reconfigure ASconfig
@01:19:57.82 AS.seek 0.5ml
@01:20:26.86 delay 1 second
@01:20:27.88 AFV.select :holdingCoil
@01:20:27.99 ATV.dial ASV
@01:20:28.00 ASV.dial :SPErecovery
@01:20:28.67 AS.reconfigure ASslow0
@01:20:28.73 AS.seek 0.3ml
@01:20:49.11 ATV.dial :air2
@01:20:50.04 AS.reconfigure ASslow1
@01:20:50.10 AS.seek 0.32ml
@01:20:54.46 ATV.dial ASV
@01:20:55.58 AS.reconfigure ASslow0
@01:20:55.64 AS.seek 0.57ml
@01:21:21.09 ASV.dial :wasteD
@01:21:21.70 AS.reconfigure ASnorm
@01:21:21.76 AS.seek 0ml
@01:21:50.83 AFV.select :carrierSolution
@01:21:50.95 delay 2 seconds
@01:21:52.97 AS.reconfigure ASconfig
@01:21:53.02 AS.seek 0.5ml
@01:22:22.09 delay 1 second
@01:22:23.11 AFV.select :holdingCoil
@01:22:23.22 ATV.dial ASV
@01:22:23.23 ASV.dial :SPErecovery
@01:22:23.89 AS.reconfigure ASslow0
@01:22:23.95 AS.seek 0.3ml
@01:22:44.37 ATV.dial :air2
@01:22:45.47 AS.reconfigure ASslow1
@01:22:45.53 AS.seek 0.32ml
@01:22:50.08 ATV.dial ASV
@01:22:51.20 AS.reconfigure ASslow0
@01:22:51.26 AS.seek 0.57ml
@01:23:16.72 ASV.dial :wasteD
@01:23:17.34 AS.reconfigure ASnorm
@01:23:17.40 AS.seek 0ml
@01:23:46.48 ATV.dial :air2
@01:23:47.53 AS.reconfigure ASslow0
@01:23:47.59 AS.seek 0.3ml
@01:24:18.09 ATV.dial :ASV
@01:24:19.12 ASV.dial :wasteD
@01:24:19.14 AS.reconfigure ASconfig
@01:24:19.21 AS.seek 0.05ml
@01:24:33.91 ATV.dial :waste
@01:24:35.90 AS.seek 0ml
@01:24:39.06 ASV.dial :SPEdelivery
@01:24:40.15 Cleaning PCR
@01:24:40.16 AFV.select :carrierSolution
@01:24:40.27 delay 2 seconds
@01:24:42.29 AS.seek 0.5ml
@01:25:11.31 delay 1 second
@01:25:12.33 AFV.select :holdingCoil
@01:25:12.44 ASV.dial :waste1
@01:25:12.81 ATV.dial :air3
@01:25:13.96 AS.reconfigure ASslow1
@01:25:14.02 AS.seek 0.51ml
@01:25:16.38 delay 2 seconds
@01:25:18.41 ATV.dial :bleach
@01:25:18.89 AS.seek 0.81ml
@01:26:19.52 delay 3 seconds
@01:26:22.55 ATV.dial :air3
@01:26:23.14 delay 3 seconds
@01:26:26.22 AS.seek 0.835ml
@01:26:31.54 delay 3 seconds
@01:26:34.57 ATV.dial :bleach
@01:26:35.22 AS.seek 0.82ml
@01:26:38.55 delay 3 seconds
@01:26:41.58 ARV.dial :waste
@01:26:42.20 delay 3 seconds
@01:26:45.23 ATV.dial :ARV
@01:26:46.15 AS.reconfigure ASconfig
@01:26:46.21 AS.seek 0.47ml
@01:27:06.61 AFV.select :carrierSolution
@01:27:06.73 delay 2 seconds
@01:27:08.75 AS.seek 1ml
@01:27:39.53 delay 1 second
@01:27:40.55 AFV.select :holdingCoil
@01:27:40.66 delay 1 second
@01:27:41.68 AS.reconfigure ASfast
@01:27:41.74 AS.seek 0ml
@01:28:09.08 AFV.select :carrierSolution
@01:28:09.20 delay 2 seconds
@01:28:11.22 AS.reconfigure ASconfig
@01:28:11.27 AS.seek 1ml
@01:29:09.03 delay 1 second
@01:29:10.05 AFV.select :holdingCoil
@01:29:10.16 delay 1 second
@01:29:11.18 AS.reconfigure ASfast
@01:29:11.24 AS.seek 0ml
@01:29:38.58 AFV.select :carrierSolution
@01:29:38.70 delay 2 seconds
@01:29:40.72 AS.reconfigure ASconfig
@01:29:40.77 AS.seek 1ml
@01:30:38.56 delay 1 second
@01:30:39.58 AFV.select :holdingCoil
@01:30:39.69 delay 1 second
@01:30:40.71 AS.reconfigure ASfast
@01:30:40.77 AS.seek 0ml
@01:31:08.13 ARV.dial between carrier and air1
@01:31:08.64 ATV.dial :air3
@01:31:10.01 AS.reconfigure ASslow1
@01:31:10.07 AS.seek 0.01ml
@01:31:12.41 delay 2 seconds
@01:31:14.44 ATV.dial :bleach
@01:31:15.08 AS.seek 0.81ml
@01:33:56.28 delay 3 seconds
@01:33:59.31 ATV.dial :air3
@01:33:59.89 delay 3 seconds
@01:34:02.96 AS.seek 0.835ml
@01:34:08.34 delay 3 seconds
@01:34:11.37 ATV.dial :bleach
@01:34:12.00 AS.seek 0.82ml
@01:34:15.30 delay 3 seconds
@01:34:18.33 ATV.dial PCR
@01:34:18.74 AS.reconfigure ASconfig
@01:34:18.79 AS.seek 0ml
@01:35:06.20 PCR.program :bleachProfile
@01:35:06.27 PCR program should complete in < 2:45
@01:35:06.28 PCR.run 10:00
@01:38:08.54 PCR.off
@01:38:08.58 AFV.select :carrierSolution
@01:38:08.70 delay 2 seconds
@01:38:10.72 AS.seek 1ml
@01:39:08.50 delay 1 second
@01:39:09.52 AFV.select :holdingCoil
@01:39:09.63 delay 1 second
@01:39:10.65 AS.reconfigure ASfast
@01:39:10.71 AS.seek 0ml
@01:39:38.05 delay 3 seconds
@01:39:41.07 AFV.select :carrierSolution
@01:39:41.18 delay 2 seconds
@01:39:43.20 AS.reconfigure ASconfig
@01:39:43.26 AS.seek 1ml
@01:40:41.02 delay 1 second
@01:40:42.04 AFV.select :holdingCoil
@01:40:42.15 delay 1 second
@01:40:43.17 AS.reconfigure ASfast
@01:40:43.23 AS.seek 0ml
@01:41:10.58 delay 3 seconds
@01:41:13.60 AFV.select :carrierSolution
@01:41:13.71 delay 2 seconds
@01:41:15.73 AS.reconfigure ASconfig
@01:41:15.80 AS.seek 1ml
@01:42:13.56 delay 1 second
@01:42:14.58 AFV.select :holdingCoil
@01:42:14.69 delay 1 second
@01:42:15.71 AS.reconfigure ASfast
@01:42:15.77 AS.seek 0ml
@01:42:43.13 delay 3 seconds
@01:42:46.15 AFV.select :carrierSolution
@01:42:46.26 delay 2 seconds
@01:42:48.28 AS.reconfigure ASconfig
@01:42:48.34 AS.seek 1ml
@01:43:46.09 delay 1 second
@01:43:47.11 AFV.select :holdingCoil
@01:43:47.22 delay 1 second
@01:43:48.24 AS.reconfigure ASfast
@01:43:48.30 AS.seek 0ml
@01:44:15.66 delay 3 seconds
@01:44:18.68 AFV.select :carrierSolution
@01:44:18.79 delay 2 seconds
@01:44:20.81 AS.reconfigure ASconfig
@01:44:20.88 AS.seek 1ml
@01:45:18.63 delay 1 second
@01:45:19.65 AFV.select :holdingCoil
@01:45:19.76 delay 1 second
@01:45:20.78 AS.reconfigure ASnorm
@01:45:20.83 AS.seek 0ml
@01:46:11.55 AFV.select :carrierSolution
@01:46:11.67 delay 2 seconds
@01:46:13.69 AS.reconfigure ASconfig
@01:46:13.74 AS.seek 1ml
@01:47:11.50 delay 1 second
@01:47:12.52 AFV.select :holdingCoil
@01:47:12.63 delay 1 second
@01:47:13.65 ATV.dial :waste
@01:47:14.95 AS.reconfigure ASnorm
@01:47:15.01 AS.seek 0.5ml
@01:47:40.58 ATV.dial :waste2
@01:47:41.95 AS.seek 0ml
@01:48:07.55 <bkha> Session Ended
@11:50:41.00CET25-Mar-21 <MAIN> ESP2 v3.30 I2C Gateway v3.06 for core bus
@11:50:43.11 
Configuration for mer 7/20/20 brent@mbari.org
/home/esp/esp2/type/shallow/mer/configure.rb
@11:50:43.20 
Configuration for MFB3 by preston@mbari.org
/home/esp/esp2/type/mfb/MFB3/configure.rb
@11:50:48.41 Polling Can every 10:00
@11:50:50.13 CS.configure :default
@11:50:50.17 CS.hold
@11:50:50.21 CS.configure :default
@11:50:50.29 PS.configure :default
@11:50:50.38 SS.configure :default
@11:50:50.41 SS.hold
@11:50:50.47 SS.configure :default
@11:50:50.55 CC.configure :default
@11:50:50.59 CC.hold
@11:50:50.65 CC.configure :default
@11:50:50.73 PC.configure :default
@11:50:50.77 PC.hold
@11:50:50.85 PC.configure :default
@11:50:51.05 Forearm.configure :loose
@11:50:51.13 Forearm.hold
@11:50:51.22 Forearm.configure :loose
@11:50:51.38 Elbow.configure :loose
@11:50:51.45 Elbow.hold
@11:50:51.54 Elbow.configure :loose
@11:50:51.69 SC.configure :loose
@11:50:51.72 SC.hold
@11:50:51.77 SC.configure :loose
@11:50:51.85 SE.configure :default
@11:50:51.89 SE.hold
@11:50:51.94 SE.configure :default
@11:50:51.98 CH.coast
@11:50:52.02 CH.configure :default
@11:50:52.05 PH.coast
@11:50:52.10 PH.configure :default
@11:50:52.20 SPE.coast
@11:50:52.24 SPE.configure SPEconfig
@11:50:52.32 AS.configure ASconfig
@11:50:52.36 AS.hold
@11:50:52.40 AS.configure ASconfig
@11:50:52.45 PCR.off
@11:50:52.48 PCR.pidtemp= 50,0.1,0,300,25,25
@11:50:52.51 PCR.caltemp= 2.5051,0.0372,0
@11:50:52.54 All dwarf controllers running firmware version 3.75
@11:50:52.73 Cleanly exited at 11:50:25CET25-Mar-21
@11:50:52.76 Loading /home/esp/esp2/mission/GENOMAP.rb mission
@11:50:52.90 <MFBPCR> Extracting Nucleic Acid from extSrc
@11:50:52.93 SPE.hold 2:46:40,55C
@11:50:52.96 ASV.dial :SPEdelivery
@11:50:53.45 delay 20 seconds
@11:50:53.82 <bkha> Session Begun
@11:50:56.15 -> showlog 0
@11:51:02.60 -> showlog 10
@11:51:13.47 <MFBPCR> AFV.select :holdingCoil
@11:51:13.60 ATV.dial :air2
@11:51:15.07 AS.reconfigure ASslow1
@11:51:15.13 AS.seek 0.07ml
@11:51:29.48 delay 3 seconds
@11:51:32.51 ATV.dial :extSrc
@11:51:34.03 AS.seek 0.47ml
@11:52:54.75 delay 5 seconds
@11:52:59.83 Solid Phase Extraction Heater at 39.3C
@11:52:59.84 ATV.dial ASV
@11:53:00.70 ASV.dial :SPEdelivery
@11:53:00.79 AS.seek 0.042ml
@11:54:27.17 delay 10 seconds
@11:54:37.21 Rinsing external lysate line extSrc
@11:54:37.22 AFV.select :carrierSolution
@11:54:37.34 delay 2 seconds
@11:54:39.36 AS.reconfigure ASconfig
@11:54:39.42 AS.seek 1ml
@11:55:17.78 <bkha> -> exit
@11:55:17.79 Session Ended
@11:55:34.76 <MFBPCR> delay 1 second
@11:55:35.78 AFV.select :holdingCoil
@11:55:35.89 delay 1 second
@11:55:36.91 ATV.dial :extSrc
@11:55:37.74 AS.reconfigure ASnorm
@11:55:37.78 AS.seek 0ml
@11:56:28.47 ATV.dial :air2
@11:56:30.06 AS.reconfigure ASslow0
@11:56:30.11 AS.seek 0.05ml
@11:56:35.50 ATV.dial :extSrc
@11:56:37.09 AS.seek 0.01ml
@11:56:41.45 ATV.dial :waste
@11:56:42.84 AS.reconfigure ASconfig
@11:56:42.88 AS.seek 0ml
@11:56:44.12 ethanol wash
@11:56:44.13 ATV.dial :air2
@11:56:45.31 AS.reconfigure ASslow2
@11:56:45.36 AS.seek 0.01ml
@11:56:56.11 delay 3 seconds
@11:56:59.14 Pulling 0.4ml of Ethanol Wash
@11:56:59.15 ATV.dial :ethanol
@11:57:00.03 AS.reconfigure ASslow1
@11:57:00.08 AS.seek 0.41ml
@11:58:20.84 delay 3 seconds
@11:58:23.87 ATV.dial :air1
@11:58:24.26 delay 3 seconds
@11:58:27.33 AS.seek 0.435ml
@11:58:32.66 delay 3 seconds
@11:58:35.69 ATV.dial :ethanol
@11:58:36.29 AS.seek 0.42ml
@11:58:39.54 delay 3 seconds
@11:58:42.57 ATV.dial ASV
@11:58:43.96 AS.seek 0.093ml
@11:59:49.97 delay 3 seconds
@11:59:53.00 ATV.dial :waste2
@11:59:53.79 AS.reconfigure ASconfig
@11:59:53.83 AS.seek 0ml
@11:59:59.47 ATV.dial :air2
@11:59:59.88 AS.reconfigure ASslow1
@11:59:59.92 AS.seek 1ml
@12:03:21.40 delay 5 seconds
@12:03:26.43 ATV.dial ASV
@12:03:27.47 ASV.dial :SPEdelivery
@12:03:27.49 AS.seek 0.02ml
@12:06:44.83 delay 5 seconds
@12:06:49.86 ATV.dial :air2
@12:06:50.96 AS.seek 0.52ml
@12:08:31.70 delay 5 seconds
@12:08:36.73 ATV.dial ASV
@12:08:37.78 ASV.dial :wasteR
@12:08:38.16 AS.seek 0.3ml
@12:09:22.67 delay 20 seconds
@12:09:42.71 AS.seek 0.07ml
@12:10:29.22 delay 20 seconds
@12:10:49.25 ATV.dial :waste2
@12:10:50.07 AS.reconfigure ASconfig
@12:10:50.11 AS.seek 0ml
@12:10:54.42 Elution
@12:10:54.43 ATV.dial :air1
@12:10:55.59 AS.reconfigure ASslow1
@12:10:55.63 AS.seek 0.05ml
@12:11:06.01 delay 3 seconds
@12:11:09.04 ATV.dial :ElutionWater
@12:11:09.75 AS.seek 0.11ml
@12:11:22.15 delay 3 seconds
@12:11:25.18 ATV.dial ASV,via: :air2
@12:11:27.41 ASV.dial :SPEelution
@12:11:28.01 AFV.select :carrierSolution
@12:11:28.16 AS.reconfigure ASnorm
@12:11:28.21 AS.seek 0.76ml
@12:12:01.33 delay 3 seconds
@12:12:04.35 AFV.select :holdingCoil
@12:12:04.50 AS.reconfigure ASslow1
@12:12:04.54 AS.seek 0.475ml
@12:13:02.19 delay 5 seconds
@12:13:07.27 AS.reconfigure ASslow2
@12:13:07.32 AS.seek 0.44ml
@12:13:42.78 delay 1:00
@12:14:42.82 pushing eluate to holding coil
@12:14:42.87 AS.seek 0.28ml
@12:17:24.14 delay 30 seconds
@12:17:54.18 ATV.dial :waste,via: :air3
@12:17:56.01 AS.reconfigure ASconfig
@12:17:56.05 AS.seek 0ml
@12:18:12.40 Parking Bleach in LysateSource line and SPEcolumn
@12:18:12.41 ATV.dial :air3
@12:18:13.29 AS.reconfigure ASslow1
@12:18:13.33 AS.seek 0.03ml
@12:18:19.78 ATV.dial :ElutionWater
@12:18:20.42 AS.seek 0.06ml
@12:18:26.76 ATV.dial :air3
@12:18:27.40 AS.seek 0.07ml
@12:18:29.65 delay 2 seconds
@12:18:31.68 ATV.dial :bleach
@12:18:32.15 AS.seek 0.15ml
@12:18:48.47 delay 3 seconds
@12:18:51.50 ATV.dial :air3
@12:18:52.08 delay 3 seconds
@12:18:55.15 AS.seek 0.175ml
@12:19:00.45 delay 3 seconds
@12:19:03.48 ATV.dial :bleach
@12:19:04.11 AS.seek 0.16ml
@12:19:07.42 delay 3 seconds
@12:19:10.45 ATV.dial :extSrc
@12:19:11.09 AS.reconfigure ASconfig
@12:19:11.13 AS.seek 0.015ml
@12:19:19.76 ATV.dial :waste
@12:19:21.29 AS.seek 0ml
@12:19:22.42 ATV.dial :air3
@12:19:23.31 AS.reconfigure ASslow1
@12:19:23.35 AS.seek 0.01ml
@12:19:25.73 delay 2 seconds
@12:19:27.76 ATV.dial :bleach
@12:19:28.21 AS.seek 0.58ml
@12:21:23.11 delay 3 seconds
@12:21:26.14 ATV.dial :air3
@12:21:26.72 delay 3 seconds
@12:21:29.79 AS.seek 0.605ml
@12:21:35.09 delay 3 seconds
@12:21:38.12 ATV.dial :bleach
@12:21:38.76 AS.seek 0.59ml
@12:21:42.06 delay 3 seconds
@12:21:45.09 ATV.dial :ASV
@12:21:46.17 ASV.dial :wasteR
@12:21:46.79 AS.seek 0ml
@12:23:45.78 delay 1:00
@12:24:45.81 AFV.select :carrierSolution
@12:24:45.92 delay 2 seconds
@12:24:47.94 AS.reconfigure ASconfig
@12:24:47.99 AS.seek 1ml
@12:25:45.73 delay 1 second
@12:25:46.75 AFV.select :holdingCoil
@12:25:46.86 delay 1 second
@12:25:47.88 ATV.dial ASV
@12:25:47.89 ASV.dial :wasteR
@12:25:47.90 AS.reconfigure ASslow1
@12:25:47.95 AS.seek 0ml
@12:29:09.29 delay 20 seconds
@12:29:29.33 ATV.dial :air3
@12:29:30.92 AS.seek 0.59ml
@12:31:29.76 delay 3 seconds
@12:31:32.79 ATV.dial :ASV
@12:31:34.30 ASV.dial :wasteR
@12:31:34.37 AS.seek 0.09ml
@12:33:15.22 delay 30 seconds
@12:33:45.26 ATV.dial :extSrc
@12:33:46.06 delay 3 seconds
@12:33:49.09 ATV.dial :ElutionWater
@12:33:50.29 AS.seek 0.12ml
@12:33:56.61 ATV.dial :air3
@12:33:57.25 AS.seek 0.13ml
@12:33:59.54 delay 2 seconds
@12:34:01.57 ATV.dial :bleach
@12:34:02.05 AS.seek 0.23ml
@12:34:22.48 delay 3 seconds
@12:34:25.51 ATV.dial :air3
@12:34:26.09 delay 3 seconds
@12:34:29.17 AS.seek 0.255ml
@12:34:34.48 delay 3 seconds
@12:34:37.51 ATV.dial :bleach
@12:34:38.14 AS.seek 0.24ml
@12:34:41.44 delay 3 seconds
@12:34:44.47 ATV.dial :ASV
@12:34:45.56 ASV.dial :SPEdelivery
@12:34:46.37 AFV.select :carrierSolution
@12:34:46.48 delay 2 seconds
@12:34:48.51 AS.reconfigure ASconfig
@12:34:48.56 AS.seek 0.5ml
@12:35:03.79 delay 1 second
@12:35:04.81 AFV.select :holdingCoil
@12:35:04.96 AS.reconfigure ASslow1
@12:35:05.01 AS.seek 0.17ml
@12:36:11.63 SPE.hold 2:46:40,95C
@12:36:11.68 delay 3:00
@12:39:11.72 AFV.select :carrierSolution
@12:39:11.83 delay 2 seconds
@12:39:13.85 AS.reconfigure ASconfig
@12:39:13.90 AS.seek 1ml
@12:40:01.89 delay 1 second
@12:40:02.91 AFV.select :holdingCoil
@12:40:03.02 delay 1 second
@12:40:04.04 ASV.dial :wasteD
@12:40:05.15 AS.seek 0ml
@12:41:02.89 Priming Eluate
@12:41:02.90 AFV.select :carrierSolution
@12:41:03.01 delay 2 seconds
@12:41:05.03 AS.seek 0.5ml
@12:41:34.06 delay 1 second
@12:41:35.08 AFV.select :holdingCoil
@12:41:35.19 ATV.dial ASV
@12:41:35.20 ASV.dial :SPErecovery
@12:41:35.81 Swish #1 of 2
@12:41:35.88 AS.reconfigure ASslow0
@12:41:35.92 AS.seek 0.6ml
@12:41:46.39 AS.seek 0.5ml
@12:41:56.73 Swish #2 of 2
@12:41:56.80 AS.seek 0.6ml
@12:42:07.28 AS.seek 0.5ml
@12:42:17.64 delay 3 seconds
@12:42:20.71 AS.reconfigure ASslow1
@12:42:20.76 AS.seek 0.684ml
@12:42:58.01 delay 3 seconds
@12:43:01.04 Eluate primed
@12:43:01.05 ATV.dial :waste2
@12:43:01.71 AS.reconfigure ASnorm
@12:43:01.75 AS.seek 0ml
@12:43:36.54 SPE.coast
@12:43:36.61 ASV.dial :SPErecovery
@12:43:36.63 Setting up PCR for ACYPP2 -- writing /var/log/esp/ACYPP221mar2512h.pcr
@12:43:36.64 Building PCR Slug from ASV with MM1 mix, PP2 primer
@12:43:36.65 Priming with carrier
@12:43:36.66 ATV.dial ARV
@12:43:37.69 ARV.dial :carrier
@12:43:38.03 AS.seek 0.5ml
@12:44:03.56 AFV.select :carrierSolution
@12:44:03.67 delay 2 seconds
@12:44:05.69 AS.reconfigure ASconfig
@12:44:05.74 AS.seek 1ml
@12:44:34.76 delay 1 second
@12:44:35.79 AFV.select :holdingCoil
@12:44:35.89 delay 1 second
@12:44:36.91 ATV.dial :waste
@12:44:38.52 AS.reconfigure ASnorm
@12:44:38.56 AS.seek 0ml
@12:45:29.23 AFV.select :carrierSolution
@12:45:29.34 delay 2 seconds
@12:45:31.36 AS.reconfigure ASconfig
@12:45:31.41 AS.seek 0.5ml
@12:46:00.47 delay 1 second
@12:46:01.49 AFV.select :holdingCoil
@12:46:01.60 Building Slug
@12:46:01.61 ATV.dial :ARV
@12:46:03.37 delay 2 seconds
@12:46:05.39 ARV.dial :air1
@12:46:05.77 delay 2 seconds
@12:46:07.84 AS.reconfigure ASslow2
@12:46:07.89 AS.seek 0.506ml
@12:46:14.34 delay 2 seconds
@12:46:16.37 ARV.dial :MM1
@12:46:16.98 delay 2 seconds
@12:46:19.01 Adding MM1
@12:46:19.07 AS.seek 0.524ml
@12:46:37.39 delay 25 seconds
@12:47:02.42 ARV.dial :PP2
@12:47:02.82 Adding PP2
@12:47:02.83 delay 2 seconds
@12:47:04.90 AS.seek 0.53ml
@12:47:11.54 delay 10 seconds
@12:47:21.58 ARV.dial :air2
@12:47:21.99 adding ARV air
@12:47:22.00 delay 2 seconds
@12:47:24.07 AS.seek 0.536ml
@12:47:30.36 delay 5 seconds
@12:47:35.39 ARV.dial :carrier
@12:47:36.72 delay 7 seconds
@12:47:43.80 AS.seek 0.594ml
@12:48:42.39 delay 5 seconds
@12:48:47.42 ATV.dial :ASV
@12:48:47.83 delay 2 seconds
@12:48:49.91 AS.seek 0.6ml
@12:48:56.20 delay 4 seconds
@12:49:00.23 ATV.dial ARV
@12:49:00.80 delay 2 seconds
@12:49:02.83 ARV.dial :carrier
@12:49:02.84 delay 2 seconds
@12:49:04.91 AS.seek 0.65ml
@12:49:55.47 delay 4 seconds
@12:49:59.50 ATV.dial PCR
@12:50:00.12 ASV.dial :waste1
@12:50:00.93 ARV.dial :air1
@12:50:01.34 Positioning Slug by 0.324ml
@12:50:01.41 AS.seek 0.326ml
@12:55:27.36 delay 5 seconds
@12:55:32.39 ATV.dial ARV
@12:55:33.19 ASV.dial :PCRlock
@12:55:33.56 delay 5 seconds
@12:55:38.59 Running ACYPP2 Profile60CIQ2 thermocycling
@12:55:38.60 <PCR> PCR.program Profile60CIQ2
@12:55:38.71 Profile60CIQ2 should complete in < 1:47:50
@12:55:38.72 PCR.run 2:19:47.5
@14:24:13.49 PCR.off
@14:24:14.10 <MFBPCR> Running ACYPP2 MeltProfile60 thermocycling
@14:24:14.11 <PCR> PCR.program MeltProfile60
@14:24:14.56 MeltProfile60 should complete in < 11:55
@14:24:14.57 PCR.run 19:53.75
@14:31:52.39 PCR.off
@14:31:53.31 <MFBPCR> PCR for ACYPP2 completed -- closed /var/log/esp/ACYPP221mar2512h.pcr
@14:31:53.37 Cleaning PCR
@14:31:53.38 AFV.select :carrierSolution
@14:31:53.48 delay 2 seconds
@14:31:55.50 AS.reconfigure ASconfig
@14:31:55.55 AS.seek 0.5ml
@14:32:05.81 delay 1 second
@14:32:06.83 AFV.select :holdingCoil
@14:32:06.94 ASV.dial :waste1
@14:32:07.33 ATV.dial :air3
@14:32:08.68 AS.reconfigure ASslow1
@14:32:08.73 AS.seek 0.51ml
@14:32:10.99 delay 2 seconds
@14:32:13.02 ATV.dial :bleach
@14:32:13.67 AS.seek 0.81ml
@14:33:14.36 delay 3 seconds
@14:33:17.39 ATV.dial :air3
@14:33:17.95 delay 3 seconds
@14:33:21.03 AS.seek 0.835ml
@14:33:26.33 delay 3 seconds
@14:33:29.36 ATV.dial :bleach
@14:33:29.98 AS.seek 0.82ml
@14:33:33.30 delay 3 seconds
@14:33:36.33 ARV.dial :waste
@14:33:36.95 delay 3 seconds
@14:33:39.98 ATV.dial :ARV
@14:33:40.89 AS.reconfigure ASconfig
@14:33:40.93 AS.seek 0.47ml
@14:34:01.31 AFV.select :carrierSolution
@14:34:01.42 delay 2 seconds
@14:34:03.44 AS.seek 1ml
@14:34:34.22 delay 1 second
@14:34:35.24 AFV.select :holdingCoil
@14:34:35.35 delay 1 second
@14:34:36.37 AS.reconfigure ASfast
@14:34:36.42 AS.seek 0ml
@14:35:03.75 AFV.select :carrierSolution
@14:35:03.86 delay 2 seconds
@14:35:05.88 AS.reconfigure ASconfig
@14:35:05.93 AS.seek 1ml
@14:36:03.67 delay 1 second
@14:36:04.69 AFV.select :holdingCoil
@14:36:04.80 delay 1 second
@14:36:05.82 AS.reconfigure ASfast
@14:36:05.87 AS.seek 0ml
@14:36:33.19 AFV.select :carrierSolution
@14:36:33.30 delay 2 seconds
@14:36:35.32 AS.reconfigure ASconfig
@14:36:35.37 AS.seek 1ml
@14:37:33.12 delay 1 second
@14:37:34.14 AFV.select :holdingCoil
@14:37:34.25 delay 1 second
@14:37:35.27 AS.reconfigure ASfast
@14:37:35.32 AS.seek 0ml
@14:38:02.65 ARV.dial between carrier and air1
@14:38:03.16 ATV.dial :air3
@14:38:04.51 AS.reconfigure ASslow1
@14:38:04.55 AS.seek 0.01ml
@14:38:06.83 delay 2 seconds
@14:38:08.86 ATV.dial :bleach
@14:38:09.50 AS.seek 0.81ml
@14:40:50.70 delay 3 seconds
@14:40:53.73 ATV.dial :air3
@14:40:54.31 delay 3 seconds
@14:40:57.38 AS.seek 0.835ml
@14:41:02.75 delay 3 seconds
@14:41:05.78 ATV.dial :bleach
@14:41:06.40 AS.seek 0.82ml
@14:41:09.67 delay 3 seconds
@14:41:12.70 ATV.dial PCR
@14:41:13.12 AS.reconfigure ASconfig
@14:41:13.16 AS.seek 0ml
@14:42:00.56 PCR.program :bleachProfile
@14:42:00.63 PCR program should complete in < 2:45
@14:42:00.64 PCR.run 10:00
@14:44:56.83 PCR.off
@14:44:56.86 AFV.select :carrierSolution
@14:44:56.97 delay 2 seconds
@14:44:58.99 AS.seek 1ml
@14:45:56.75 delay 1 second
@14:45:57.77 AFV.select :holdingCoil
@14:45:57.88 delay 1 second
@14:45:58.90 AS.reconfigure ASfast
@14:45:58.95 AS.seek 0ml
@14:46:26.28 delay 3 seconds
@14:46:29.30 AFV.select :carrierSolution
@14:46:29.41 delay 2 seconds
@14:46:31.43 AS.reconfigure ASconfig
@14:46:31.48 AS.seek 1ml
@14:47:29.20 delay 1 second
@14:47:30.22 AFV.select :holdingCoil
@14:47:30.33 delay 1 second
@14:47:31.35 AS.reconfigure ASfast
@14:47:31.40 AS.seek 0ml
@14:47:58.70 delay 3 seconds
@14:48:01.72 AFV.select :carrierSolution
@14:48:01.83 delay 2 seconds
@14:48:03.85 AS.reconfigure ASconfig
@14:48:03.90 AS.seek 1ml
@14:49:01.67 delay 1 second
@14:49:02.69 AFV.select :holdingCoil
@14:49:02.80 delay 1 second
@14:49:03.82 AS.reconfigure ASfast
@14:49:03.87 AS.seek 0ml
@14:49:31.19 delay 3 seconds
@14:49:34.21 AFV.select :carrierSolution
@14:49:34.32 delay 2 seconds
@14:49:36.34 AS.reconfigure ASconfig
@14:49:36.39 AS.seek 1ml
@14:50:34.17 delay 1 second
@14:50:35.19 AFV.select :holdingCoil
@14:50:35.30 delay 1 second
@14:50:36.32 AS.reconfigure ASfast
@14:50:36.37 AS.seek 0ml
@14:51:03.69 delay 3 seconds
@14:51:06.71 AFV.select :carrierSolution
@14:51:06.82 delay 2 seconds
@14:51:08.84 AS.reconfigure ASconfig
@14:51:08.90 AS.seek 1ml
@14:52:06.62 delay 1 second
@14:52:07.64 AFV.select :holdingCoil
@14:52:07.75 delay 1 second
@14:52:08.77 AS.reconfigure ASnorm
@14:52:08.82 AS.seek 0ml
@14:52:59.56 Setting up PCR for AOPP1 -- writing /var/log/esp/AOPP121mar2514h.pcr
@14:52:59.57 Building PCR Slug from ASV with MM1 mix, PP1 primer
@14:52:59.58 Priming with carrier
@14:52:59.59 ATV.dial ARV
@14:53:00.22 ARV.dial :carrier
@14:53:00.54 AS.seek 0.5ml
@14:53:26.11 AFV.select :carrierSolution
@14:53:26.22 delay 2 seconds
@14:53:28.24 AS.reconfigure ASconfig
@14:53:28.29 AS.seek 1ml
@14:53:57.28 delay 1 second
@14:53:58.30 AFV.select :holdingCoil
@14:53:58.41 delay 1 second
@14:53:59.43 ATV.dial :waste
@14:54:01.20 AS.reconfigure ASnorm
@14:54:01.24 AS.seek 0ml
@14:54:51.92 AFV.select :carrierSolution
@14:54:52.03 delay 2 seconds
@14:54:54.05 AS.reconfigure ASconfig
@14:54:54.10 AS.seek 0.5ml
@14:55:23.14 delay 1 second
@14:55:24.16 AFV.select :holdingCoil
@14:55:24.27 Building Slug
@14:55:24.28 ATV.dial :ARV
@14:55:26.03 delay 2 seconds
@14:55:28.06 ARV.dial :air1
@14:55:28.45 delay 2 seconds
@14:55:30.52 AS.reconfigure ASslow2
@14:55:30.57 AS.seek 0.506ml
@14:55:37.05 delay 2 seconds
@14:55:39.08 ARV.dial :MM1
@14:55:39.70 delay 2 seconds
@14:55:41.73 Adding MM1
@14:55:41.79 AS.seek 0.524ml
@14:56:00.50 delay 25 seconds
@14:56:25.53 ARV.dial :PP1
@14:56:25.91 Adding PP1
@14:56:25.92 delay 2 seconds
@14:56:27.99 AS.seek 0.53ml
@14:56:34.34 delay 10 seconds
@14:56:44.38 ARV.dial :air1
@14:56:44.79 adding ARV air
@14:56:44.80 delay 2 seconds
@14:56:46.87 AS.seek 0.536ml
@14:56:53.39 delay 5 seconds
@14:56:58.42 ARV.dial :carrier
@14:56:58.83 delay 7 seconds
@14:57:05.92 AS.seek 0.594ml
@14:58:04.53 delay 5 seconds
@14:58:09.56 ATV.dial :ASV
@14:58:09.95 delay 2 seconds
@14:58:12.03 AS.seek 0.6ml
@14:58:18.26 delay 4 seconds
@14:58:22.29 ATV.dial ARV
@14:58:22.84 delay 2 seconds
@14:58:24.87 ARV.dial :carrier
@14:58:24.88 delay 2 seconds
@14:58:26.95 AS.seek 0.65ml
@14:59:17.45 delay 4 seconds
@14:59:21.48 ATV.dial PCR
@14:59:22.11 ASV.dial :waste1
@14:59:22.13 ARV.dial :air1
@14:59:22.53 Positioning Slug by 0.324ml
@14:59:22.60 AS.seek 0.326ml
@15:04:49.03 delay 5 seconds
@15:04:54.06 ATV.dial ARV
@15:04:54.86 ASV.dial :PCRlock
@15:04:55.25 delay 5 seconds
@15:05:00.70 Running AOPP1 Profile60CIQ1 thermocycling
@15:05:00.72 <PCR> PCR.program Profile60CIQ1
@15:05:00.83 Profile60CIQ1 should complete in < 1:56:15
@15:05:00.84 PCR.run 2:30:18.75
@16:44:05.12 PCR.off
@16:44:05.67 <MFBPCR> Running AOPP1 MeltProfile60 thermocycling
@16:44:05.68 <PCR> PCR.program MeltProfile60
@16:44:05.77 MeltProfile60 should complete in < 11:55
@16:44:05.78 PCR.run 19:53.75
@16:51:34.09 PCR.off
@16:51:35.01 <MFBPCR> PCR for AOPP1 completed -- closed /var/log/esp/AOPP121mar2514h.pcr
@16:51:35.07 Cleaning PCR
@16:51:35.08 AFV.select :carrierSolution
@16:51:35.18 delay 2 seconds
@16:51:37.20 AS.reconfigure ASconfig
@16:51:37.25 AS.seek 0.5ml
@16:51:47.55 delay 1 second
@16:51:48.57 AFV.select :holdingCoil
@16:51:48.68 ASV.dial :waste1
@16:51:49.06 ATV.dial :air3
@16:51:50.42 AS.reconfigure ASslow1
@16:51:50.46 AS.seek 0.51ml
@16:51:52.74 delay 2 seconds
@16:51:54.76 ATV.dial :bleach
@16:51:55.40 AS.seek 0.81ml
@16:52:55.97 delay 3 seconds
@16:52:59.00 ATV.dial :air3
@16:52:59.56 delay 3 seconds
@16:53:02.63 AS.seek 0.835ml
@16:53:07.93 delay 3 seconds
@16:53:10.96 ATV.dial :bleach
@16:53:11.59 AS.seek 0.82ml
@16:53:14.89 delay 3 seconds
@16:53:17.92 ARV.dial :waste
@16:53:18.53 delay 3 seconds
@16:53:21.56 ATV.dial :ARV
@16:53:22.48 AS.reconfigure ASconfig
@16:53:22.52 AS.seek 0.47ml
@16:53:42.89 AFV.select :carrierSolution
@16:53:43.00 delay 2 seconds
@16:53:45.02 AS.seek 1ml
@16:54:15.75 delay 1 second
@16:54:16.77 AFV.select :holdingCoil
@16:54:16.88 delay 1 second
@16:54:17.90 AS.reconfigure ASfast
@16:54:17.95 AS.seek 0ml
@16:54:45.28 AFV.select :carrierSolution
@16:54:45.39 delay 2 seconds
@16:54:47.41 AS.reconfigure ASconfig
@16:54:47.46 AS.seek 1ml
@16:55:45.19 delay 1 second
@16:55:46.21 AFV.select :holdingCoil
@16:55:46.32 delay 1 second
@16:55:47.34 AS.reconfigure ASfast
@16:55:47.39 AS.seek 0ml
@16:56:14.70 AFV.select :carrierSolution
@16:56:14.81 delay 2 seconds
@16:56:16.83 AS.reconfigure ASconfig
@16:56:16.88 AS.seek 1ml
@16:57:14.61 delay 1 second
@16:57:15.63 AFV.select :holdingCoil
@16:57:15.74 delay 1 second
@16:57:16.76 AS.reconfigure ASfast
@16:57:16.81 AS.seek 0ml
@16:57:44.12 ARV.dial between carrier and air1
@16:57:44.65 ATV.dial :air3
@16:57:46.00 AS.reconfigure ASslow1
@16:57:46.04 AS.seek 0.01ml
@16:57:48.31 delay 2 seconds
@16:57:50.34 ATV.dial :bleach
@16:57:50.98 AS.seek 0.81ml
@17:00:32.09 delay 3 seconds
@17:00:35.12 ATV.dial :air3
@17:00:35.69 delay 3 seconds
@17:00:38.76 AS.seek 0.835ml
@17:00:44.08 delay 3 seconds
@17:00:47.11 ATV.dial :bleach
@17:00:47.73 AS.seek 0.82ml
@17:00:51.15 delay 3 seconds
@17:00:54.18 ATV.dial PCR
@17:00:54.60 AS.reconfigure ASconfig
@17:00:54.64 AS.seek 0ml
@17:01:42.03 PCR.program :bleachProfile
@17:01:42.09 PCR program should complete in < 2:45
@17:01:42.10 PCR.run 10:00
@17:04:38.28 PCR.off
@17:04:38.30 AFV.select :carrierSolution
@17:04:38.41 delay 2 seconds
@17:04:40.43 AS.seek 1ml
@17:05:38.19 delay 1 second
@17:05:39.21 AFV.select :holdingCoil
@17:05:39.32 delay 1 second
@17:05:40.34 AS.reconfigure ASfast
@17:05:40.39 AS.seek 0ml
@17:06:07.73 delay 3 seconds
@17:06:10.75 AFV.select :carrierSolution
@17:06:10.86 delay 2 seconds
@17:06:12.88 AS.reconfigure ASconfig
@17:06:12.94 AS.seek 1ml
@17:07:10.69 delay 1 second
@17:07:11.71 AFV.select :holdingCoil
@17:07:11.82 delay 1 second
@17:07:12.84 AS.reconfigure ASfast
@17:07:12.89 AS.seek 0ml
@17:07:40.23 delay 3 seconds
@17:07:43.25 AFV.select :carrierSolution
@17:07:43.36 delay 2 seconds
@17:07:45.38 AS.reconfigure ASconfig
@17:07:45.44 AS.seek 1ml
@17:08:43.17 delay 1 second
@17:08:44.19 AFV.select :holdingCoil
@17:08:44.30 delay 1 second
@17:08:45.32 AS.reconfigure ASfast
@17:08:45.37 AS.seek 0ml
@17:09:12.69 delay 3 seconds
@17:09:15.71 AFV.select :carrierSolution
@17:09:15.82 delay 2 seconds
@17:09:17.84 AS.reconfigure ASconfig
@17:09:17.89 AS.seek 1ml
@17:10:15.64 delay 1 second
@17:10:16.66 AFV.select :holdingCoil
@17:10:16.77 delay 1 second
@17:10:17.79 AS.reconfigure ASfast
@17:10:17.84 AS.seek 0ml
@17:10:45.15 delay 3 seconds
@17:10:48.17 AFV.select :carrierSolution
@17:10:48.28 delay 2 seconds
@17:10:50.30 AS.reconfigure ASconfig
@17:10:50.36 AS.seek 1ml
@17:11:48.11 delay 1 second
@17:11:49.13 AFV.select :holdingCoil
@17:11:49.24 delay 1 second
@17:11:50.26 AS.reconfigure ASnorm
@17:11:50.31 AS.seek 0ml
@17:12:41.04 Setting up PCR for AARPP4 -- writing /var/log/esp/AARPP421mar2517h.pcr
@17:12:41.05 Building PCR Slug from ASV with MM2 mix, PP4 primer
@17:12:41.06 Priming with carrier
@17:12:41.07 ATV.dial ARV
@17:12:41.70 ARV.dial :carrier
@17:12:42.04 AS.seek 0.5ml
@17:13:07.56 AFV.select :carrierSolution
@17:13:07.67 delay 2 seconds
@17:13:09.69 AS.reconfigure ASconfig
@17:13:09.74 AS.seek 1ml
@17:13:38.75 delay 1 second
@17:13:39.78 AFV.select :holdingCoil
@17:13:39.88 delay 1 second
@17:13:40.90 ATV.dial :waste
@17:13:42.68 AS.reconfigure ASnorm
@17:13:42.72 AS.seek 0ml
@17:14:33.41 AFV.select :carrierSolution
@17:14:33.52 delay 2 seconds
@17:14:35.54 AS.reconfigure ASconfig
@17:14:35.59 AS.seek 0.5ml
@17:15:04.64 delay 1 second
@17:15:05.66 AFV.select :holdingCoil
@17:15:05.77 Building Slug
@17:15:05.78 ATV.dial :ARV
@17:15:07.53 delay 2 seconds
@17:15:09.56 ARV.dial :air1
@17:15:09.95 delay 2 seconds
@17:15:12.02 AS.reconfigure ASslow2
@17:15:12.07 AS.seek 0.506ml
@17:15:18.53 delay 2 seconds
@17:15:20.56 ARV.dial :MM2
@17:15:22.12 delay 2 seconds
@17:15:24.15 Adding MM2
@17:15:24.21 AS.seek 0.524ml
@17:15:42.76 delay 25 seconds
@17:16:07.79 ARV.dial :PP4
@17:16:08.20 Adding PP4
@17:16:08.21 delay 2 seconds
@17:16:10.27 AS.seek 0.53ml
@17:16:16.44 delay 10 seconds
@17:16:26.48 ARV.dial :air3
@17:16:26.87 adding ARV air
@17:16:26.88 delay 2 seconds
@17:16:28.94 AS.seek 0.536ml
@17:16:35.19 delay 5 seconds
@17:16:40.22 ARV.dial :carrier
@17:16:41.53 delay 7 seconds
@17:16:48.61 AS.seek 0.594ml
@17:17:47.01 delay 5 seconds
@17:17:52.04 ATV.dial :ASV
@17:17:52.44 delay 2 seconds
@17:17:54.92 AS.seek 0.6ml
@17:18:01.11 delay 4 seconds
@17:18:05.14 ATV.dial ARV
@17:18:05.81 delay 2 seconds
@17:18:07.84 ARV.dial :carrier
@17:18:07.85 delay 2 seconds
@17:18:09.92 AS.seek 0.65ml
@17:19:01.03 delay 4 seconds
@17:19:05.06 ATV.dial PCR
@17:19:05.69 ASV.dial :waste1
@17:19:05.71 ARV.dial :air1
@17:19:06.11 Positioning Slug by 0.324ml
@17:19:06.18 AS.seek 0.326ml
@17:24:32.81 delay 5 seconds
@17:24:37.84 ATV.dial ARV
@17:24:38.64 ASV.dial :PCRlock
@17:24:39.04 delay 5 seconds
@17:24:44.06 Running AARPP4 Profile58CIQ2 thermocycling
@17:24:44.07 <PCR> PCR.program Profile58CIQ2
@17:24:44.17 Profile58CIQ2 should complete in < 1:36:15
@17:24:44.18 PCR.run 2:05:18.75
@18:45:22.77 PCR.off
@18:45:23.31 <MFBPCR> Running AARPP4 MeltProfile58 thermocycling
@18:45:23.32 <PCR> PCR.program MeltProfile58
@18:45:23.41 MeltProfile58 should complete in < 12:33
@18:45:23.42 PCR.run 20:41.25
@18:52:53.72 PCR.off
@18:52:54.69 <MFBPCR> PCR for AARPP4 completed -- closed /var/log/esp/AARPP421mar2517h.pcr
@18:52:54.75 Cleaning PCR
@18:52:54.76 AFV.select :carrierSolution
@18:52:54.86 delay 2 seconds
@18:52:56.88 AS.reconfigure ASconfig
@18:52:56.93 AS.seek 0.5ml
@18:53:07.22 delay 1 second
@18:53:08.24 AFV.select :holdingCoil
@18:53:08.35 ASV.dial :waste1
@18:53:08.73 ATV.dial :air3
@18:53:10.09 AS.reconfigure ASslow1
@18:53:10.13 AS.seek 0.51ml
@18:53:12.43 delay 2 seconds
@18:53:14.45 ATV.dial :bleach
@18:53:15.09 AS.seek 0.81ml
@18:54:15.64 delay 3 seconds
@18:54:18.67 ATV.dial :air3
@18:54:19.23 delay 3 seconds
@18:54:22.30 AS.seek 0.835ml
@18:54:27.60 delay 3 seconds
@18:54:30.63 ATV.dial :bleach
@18:54:31.26 AS.seek 0.82ml
@18:54:34.56 delay 3 seconds
@18:54:37.59 ARV.dial :waste
@18:54:38.20 delay 3 seconds
@18:54:41.23 ATV.dial :ARV
@18:54:42.15 AS.reconfigure ASconfig
@18:54:42.19 AS.seek 0.47ml
@18:55:02.59 AFV.select :carrierSolution
@18:55:02.70 delay 2 seconds
@18:55:04.72 AS.seek 1ml
@18:55:35.45 delay 1 second
@18:55:36.47 AFV.select :holdingCoil
@18:55:36.58 delay 1 second
@18:55:37.60 AS.reconfigure ASfast
@18:55:37.65 AS.seek 0ml
@18:56:04.97 AFV.select :carrierSolution
@18:56:05.08 delay 2 seconds
@18:56:07.10 AS.reconfigure ASconfig
@18:56:07.15 AS.seek 1ml
@18:57:04.94 delay 1 second
@18:57:05.96 AFV.select :holdingCoil
@18:57:06.07 delay 1 second
@18:57:07.09 AS.reconfigure ASfast
@18:57:07.14 AS.seek 0ml
@18:57:34.47 AFV.select :carrierSolution
@18:57:34.58 delay 2 seconds
@18:57:36.60 AS.reconfigure ASconfig
@18:57:36.65 AS.seek 1ml
@18:58:34.39 delay 1 second
@18:58:35.41 AFV.select :holdingCoil
@18:58:35.52 delay 1 second
@18:58:36.54 AS.reconfigure ASfast
@18:58:36.59 AS.seek 0ml
@18:59:03.92 ARV.dial between carrier and air1
@18:59:04.44 ATV.dial :air3
@18:59:05.79 AS.reconfigure ASslow1
@18:59:05.83 AS.seek 0.01ml
@18:59:08.22 delay 2 seconds
@18:59:10.25 ATV.dial :bleach
@18:59:10.87 AS.seek 0.81ml
@19:01:52.02 delay 3 seconds
@19:01:55.05 ATV.dial :air3
@19:01:55.61 delay 3 seconds
@19:01:58.68 AS.seek 0.835ml
@19:02:03.98 delay 3 seconds
@19:02:07.01 ATV.dial :bleach
@19:02:07.62 AS.seek 0.82ml
@19:02:10.91 delay 3 seconds
@19:02:13.94 ATV.dial PCR
@19:02:14.35 AS.reconfigure ASconfig
@19:02:14.39 AS.seek 0ml
@19:03:01.80 PCR.program :bleachProfile
@19:03:01.86 PCR program should complete in < 2:45
@19:03:01.87 PCR.run 10:00
@19:05:57.67 PCR.off
@19:05:57.69 AFV.select :carrierSolution
@19:05:57.80 delay 2 seconds
@19:05:59.82 AS.seek 1ml
@19:06:57.58 delay 1 second
@19:06:58.60 AFV.select :holdingCoil
@19:06:58.71 delay 1 second
@19:06:59.73 AS.reconfigure ASfast
@19:06:59.78 AS.seek 0ml
@19:07:27.11 delay 3 seconds
@19:07:30.13 AFV.select :carrierSolution
@19:07:30.24 delay 2 seconds
@19:07:32.26 AS.reconfigure ASconfig
@19:07:32.31 AS.seek 1ml
@19:08:30.08 delay 1 second
@19:08:31.11 AFV.select :holdingCoil
@19:08:31.21 delay 1 second
@19:08:32.23 AS.reconfigure ASfast
@19:08:32.28 AS.seek 0ml
@19:08:59.58 delay 3 seconds
@19:09:02.61 AFV.select :carrierSolution
@19:09:02.71 delay 2 seconds
@19:09:04.73 AS.reconfigure ASconfig
@19:09:04.78 AS.seek 1ml
@19:10:02.55 delay 1 second
@19:10:03.57 AFV.select :holdingCoil
@19:10:03.68 delay 1 second
@19:10:04.70 AS.reconfigure ASfast
@19:10:04.75 AS.seek 0ml
@19:10:32.08 delay 3 seconds
@19:10:35.11 AFV.select :carrierSolution
@19:10:35.21 delay 2 seconds
@19:10:37.23 AS.reconfigure ASconfig
@19:10:37.28 AS.seek 1ml
@19:11:35.02 delay 1 second
@19:11:36.04 AFV.select :holdingCoil
@19:11:36.15 delay 1 second
@19:11:37.17 AS.reconfigure ASfast
@19:11:37.22 AS.seek 0ml
@19:12:04.53 delay 3 seconds
@19:12:07.55 AFV.select :carrierSolution
@19:12:07.66 delay 2 seconds
@19:12:09.68 AS.reconfigure ASconfig
@19:12:09.74 AS.seek 1ml
@19:13:07.52 delay 1 second
@19:13:08.54 AFV.select :holdingCoil
@19:13:08.65 delay 1 second
@19:13:09.67 AS.reconfigure ASnorm
@19:13:09.72 AS.seek 0ml
@19:14:00.44 Setting up PCR for AALPP3 -- writing /var/log/esp/AALPP321mar2519h.pcr
@19:14:00.45 Building PCR Slug from ASV with MM2 mix, PP3 primer
@19:14:00.46 Priming with carrier
@19:14:00.47 ATV.dial ARV
@19:14:01.11 ARV.dial :carrier
@19:14:01.45 AS.seek 0.5ml
@19:14:27.06 AFV.select :carrierSolution
@19:14:27.17 delay 2 seconds
@19:14:29.19 AS.reconfigure ASconfig
@19:14:29.24 AS.seek 1ml
@19:14:58.23 delay 1 second
@19:14:59.26 AFV.select :holdingCoil
@19:14:59.36 delay 1 second
@19:15:00.38 ATV.dial :waste
@19:15:02.17 AS.reconfigure ASnorm
@19:15:02.21 AS.seek 0ml
@19:15:52.89 AFV.select :carrierSolution
@19:15:53.00 delay 2 seconds
@19:15:55.02 AS.reconfigure ASconfig
@19:15:55.07 AS.seek 0.5ml
@19:16:24.11 delay 1 second
@19:16:25.13 AFV.select :holdingCoil
@19:16:25.24 Building Slug
@19:16:25.25 ATV.dial :ARV
@19:16:26.98 delay 2 seconds
@19:16:29.01 ARV.dial :air1
@19:16:29.81 delay 2 seconds
@19:16:31.88 AS.reconfigure ASslow2
@19:16:31.93 AS.seek 0.506ml
@19:16:38.23 delay 2 seconds
@19:16:40.26 ARV.dial :MM2
@19:16:41.81 delay 2 seconds
@19:16:43.84 Adding MM2
@19:16:43.91 AS.seek 0.524ml
@19:17:02.34 delay 25 seconds
@19:17:27.37 ARV.dial :PP3
@19:17:27.76 Adding PP3
@19:17:27.77 delay 2 seconds
@19:17:29.83 AS.seek 0.53ml
@19:17:36.27 delay 10 seconds
@19:17:46.31 ARV.dial :air2
@19:17:46.71 adding ARV air
@19:17:46.72 delay 2 seconds
@19:17:48.78 AS.seek 0.536ml
@19:17:55.33 delay 5 seconds
@19:18:00.36 ARV.dial :carrier
@19:18:01.69 delay 7 seconds
@19:18:08.77 AS.seek 0.594ml
@19:19:07.42 delay 5 seconds
@19:19:12.45 ATV.dial :ASV
@19:19:12.86 delay 2 seconds
@19:19:14.93 AS.seek 0.6ml
@19:19:21.47 delay 4 seconds
@19:19:25.50 ATV.dial ARV
@19:19:26.06 delay 2 seconds
@19:19:28.09 ARV.dial :carrier
@19:19:28.10 delay 2 seconds
@19:19:30.16 AS.seek 0.65ml
@19:20:21.27 delay 4 seconds
@19:20:25.30 ATV.dial PCR
@19:20:25.94 ASV.dial :waste1
@19:20:25.96 ARV.dial :air1
@19:20:26.35 Positioning Slug by 0.324ml
@19:20:26.43 AS.seek 0.326ml
@19:25:52.52 delay 5 seconds
@19:25:57.55 ATV.dial ARV
@19:25:58.34 ASV.dial :PCRlock
@19:25:58.73 delay 5 seconds
@19:26:03.76 Running AALPP3 Profile58CIQ2 thermocycling
@19:26:03.77 <PCR> PCR.program Profile58CIQ2
@19:26:03.87 Profile58CIQ2 should complete in < 1:36:15
@19:26:03.88 PCR.run 2:05:18.75
@20:46:36.78 PCR.off
@20:46:37.33 <MFBPCR> Running AALPP3 MeltProfile58 thermocycling
@20:46:37.34 <PCR> PCR.program MeltProfile58
@20:46:37.43 MeltProfile58 should complete in < 12:33
@20:46:37.44 PCR.run 20:41.25
@20:54:07.61 PCR.off
@20:54:08.57 <MFBPCR> PCR for AALPP3 completed -- closed /var/log/esp/AALPP321mar2519h.pcr
@20:54:08.63 Cleaning PCR
@20:54:08.64 AFV.select :carrierSolution
@20:54:08.75 delay 2 seconds
@20:54:10.77 AS.reconfigure ASconfig
@20:54:10.82 AS.seek 0.5ml
@20:54:21.14 delay 1 second
@20:54:22.16 AFV.select :holdingCoil
@20:54:22.27 ASV.dial :waste1
@20:54:22.66 ATV.dial :air3
@20:54:24.00 AS.reconfigure ASslow1
@20:54:24.04 AS.seek 0.51ml
@20:54:26.32 delay 2 seconds
@20:54:28.35 ATV.dial :bleach
@20:54:28.98 AS.seek 0.81ml
@20:55:29.53 delay 3 seconds
@20:55:32.56 ATV.dial :air3
@20:55:33.13 delay 3 seconds
@20:55:36.20 AS.seek 0.835ml
@20:55:41.54 delay 3 seconds
@20:55:44.57 ATV.dial :bleach
@20:55:45.20 AS.seek 0.82ml
@20:55:48.49 delay 3 seconds
@20:55:51.52 ARV.dial :waste
@20:55:52.13 delay 3 seconds
@20:55:55.16 ATV.dial :ARV
@20:55:56.08 AS.reconfigure ASconfig
@20:55:56.12 AS.seek 0.47ml
@20:56:16.52 AFV.select :carrierSolution
@20:56:16.63 delay 2 seconds
@20:56:18.65 AS.seek 1ml
@20:56:49.41 delay 1 second
@20:56:50.43 AFV.select :holdingCoil
@20:56:50.54 delay 1 second
@20:56:51.56 AS.reconfigure ASfast
@20:56:51.61 AS.seek 0ml
@20:57:18.91 AFV.select :carrierSolution
@20:57:19.02 delay 2 seconds
@20:57:21.04 AS.reconfigure ASconfig
@20:57:21.09 AS.seek 1ml
@20:58:18.83 delay 1 second
@20:58:19.85 AFV.select :holdingCoil
@20:58:19.96 delay 1 second
@20:58:20.98 AS.reconfigure ASfast
@20:58:21.03 AS.seek 0ml
@20:58:48.33 AFV.select :carrierSolution
@20:58:48.44 delay 2 seconds
@20:58:50.46 AS.reconfigure ASconfig
@20:58:50.51 AS.seek 1ml
@20:59:48.28 delay 1 second
@20:59:49.30 AFV.select :holdingCoil
@20:59:49.41 delay 1 second
@20:59:50.43 AS.reconfigure ASfast
@20:59:50.48 AS.seek 0ml
@21:00:17.80 ARV.dial between carrier and air1
@21:00:18.31 ATV.dial :air3
@21:00:20.07 AS.reconfigure ASslow1
@21:00:20.12 AS.seek 0.01ml
@21:00:22.41 delay 2 seconds
@21:00:24.44 ATV.dial :bleach
@21:00:25.08 AS.seek 0.81ml
@21:03:06.21 delay 3 seconds
@21:03:09.23 ATV.dial :air3
@21:03:09.80 delay 3 seconds
@21:03:12.88 AS.seek 0.835ml
@21:03:18.17 delay 3 seconds
@21:03:21.20 ATV.dial :bleach
@21:03:21.83 AS.seek 0.82ml
@21:03:25.13 delay 3 seconds
@21:03:28.16 ATV.dial PCR
@21:03:28.57 AS.reconfigure ASconfig
@21:03:28.61 AS.seek 0ml
@21:04:16.02 PCR.program :bleachProfile
@21:04:16.08 PCR program should complete in < 2:45
@21:04:16.09 PCR.run 10:00
@21:07:11.87 PCR.off
@21:07:11.89 AFV.select :carrierSolution
@21:07:12.00 delay 2 seconds
@21:07:14.02 AS.seek 1ml
@21:08:11.80 delay 1 second
@21:08:12.82 AFV.select :holdingCoil
@21:08:12.93 delay 1 second
@21:08:13.95 AS.reconfigure ASfast
@21:08:14.00 AS.seek 0ml
@21:08:41.33 delay 3 seconds
@21:08:44.35 AFV.select :carrierSolution
@21:08:44.46 delay 2 seconds
@21:08:46.48 AS.reconfigure ASconfig
@21:08:46.53 AS.seek 1ml
@21:09:44.25 delay 1 second
@21:09:45.27 AFV.select :holdingCoil
@21:09:45.38 delay 1 second
@21:09:46.40 AS.reconfigure ASfast
@21:09:46.45 AS.seek 0ml
@21:10:13.77 delay 3 seconds
@21:10:16.79 AFV.select :carrierSolution
@21:10:16.90 delay 2 seconds
@21:10:18.92 AS.reconfigure ASconfig
@21:10:18.98 AS.seek 1ml
@21:11:16.72 delay 1 second
@21:11:17.74 AFV.select :holdingCoil
@21:11:17.85 delay 1 second
@21:11:18.87 AS.reconfigure ASfast
@21:11:18.92 AS.seek 0ml
@21:11:46.24 delay 3 seconds
@21:11:49.26 AFV.select :carrierSolution
@21:11:49.37 delay 2 seconds
@21:11:51.39 AS.reconfigure ASconfig
@21:11:51.44 AS.seek 1ml
@21:12:49.19 delay 1 second
@21:12:50.21 AFV.select :holdingCoil
@21:12:50.32 delay 1 second
@21:12:51.34 AS.reconfigure ASfast
@21:12:51.39 AS.seek 0ml
@21:13:18.71 delay 3 seconds
@21:13:21.73 AFV.select :carrierSolution
@21:13:21.84 delay 2 seconds
@21:13:23.86 AS.reconfigure ASconfig
@21:13:23.92 AS.seek 1ml
@21:14:21.69 delay 1 second
@21:14:22.71 AFV.select :holdingCoil
@21:14:22.82 delay 1 second
@21:14:23.84 AS.reconfigure ASnorm
@21:14:23.89 AS.seek 0ml
@21:15:14.99 Setting up PCR for ACOPP5 -- writing /var/log/esp/ACOPP521mar2521h.pcr
@21:15:15.01 Building PCR Slug from ASV with MM3 mix, PP5 primer
@21:15:15.02 Priming with carrier
@21:15:15.03 ATV.dial ARV
@21:15:15.64 ARV.dial :carrier
@21:15:15.98 AS.seek 0.5ml
@21:15:41.52 AFV.select :carrierSolution
@21:15:41.63 delay 2 seconds
@21:15:43.65 AS.reconfigure ASconfig
@21:15:43.70 AS.seek 1ml
@21:16:12.70 delay 1 second
@21:16:13.73 AFV.select :holdingCoil
@21:16:13.83 delay 1 second
@21:16:14.85 ATV.dial :waste
@21:16:16.62 AS.reconfigure ASnorm
@21:16:16.66 AS.seek 0ml
@21:17:07.38 AFV.select :carrierSolution
@21:17:07.49 delay 2 seconds
@21:17:09.51 AS.reconfigure ASconfig
@21:17:09.56 AS.seek 0.5ml
@21:17:38.56 delay 1 second
@21:17:39.59 AFV.select :holdingCoil
@21:17:39.69 Building Slug
@21:17:39.70 ATV.dial :ARV
@21:17:41.42 delay 2 seconds
@21:17:43.45 ARV.dial :air1
@21:17:43.84 delay 2 seconds
@21:17:45.91 AS.reconfigure ASslow2
@21:17:45.96 AS.seek 0.506ml
@21:17:52.20 delay 2 seconds
@21:17:54.23 ARV.dial :MM3
@21:17:55.31 delay 2 seconds
@21:17:57.34 Adding MM3
@21:17:57.41 AS.seek 0.524ml
@21:18:15.64 delay 25 seconds
@21:18:40.67 ARV.dial :PP5
@21:18:41.07 Adding PP5
@21:18:41.08 delay 2 seconds
@21:18:43.14 AS.seek 0.53ml
@21:18:49.33 delay 10 seconds
@21:18:59.37 ARV.dial :air3
@21:18:59.75 adding ARV air
@21:18:59.77 delay 2 seconds
@21:19:01.83 AS.seek 0.536ml
@21:19:08.50 delay 5 seconds
@21:19:13.53 ARV.dial :carrier
@21:19:14.85 delay 7 seconds
@21:19:21.93 AS.seek 0.594ml
@21:20:20.42 delay 5 seconds
@21:20:25.45 ATV.dial :ASV
@21:20:25.86 delay 2 seconds
@21:20:27.94 AS.seek 0.6ml
@21:20:34.20 delay 4 seconds
@21:20:38.23 ATV.dial ARV
@21:20:38.80 delay 2 seconds
@21:20:40.83 ARV.dial :carrier
@21:20:40.84 delay 2 seconds
@21:20:42.90 AS.seek 0.65ml
@21:21:33.66 delay 4 seconds
@21:21:37.69 ATV.dial PCR
@21:21:38.31 ASV.dial :waste1
@21:21:38.33 ARV.dial :air1
@21:21:38.72 Positioning Slug by 0.324ml
@21:21:38.79 AS.seek 0.326ml
@21:27:04.80 delay 5 seconds
@21:27:09.83 ATV.dial ARV
@21:27:10.63 ASV.dial :PCRlock
@21:27:11.03 delay 5 seconds
@21:27:16.05 Running ACOPP5 ProfileIPc thermocycling
@21:27:16.06 <PCR> PCR.program ProfileIPc
@21:27:16.17 ProfileIPc should complete in < 1:26:35
@21:27:16.18 PCR.run 1:53:13.75
@22:35:35.31 PCR.off
@22:35:35.85 <MFBPCR> PCR for ACOPP5 completed -- closed /var/log/esp/ACOPP521mar2521h.pcr
@22:35:35.91 Cleaning PCR
@22:35:35.92 AFV.select :carrierSolution
@22:35:36.02 delay 2 seconds
@22:35:38.04 AS.reconfigure ASconfig
@22:35:38.09 AS.seek 0.5ml
@22:35:48.38 delay 1 second
@22:35:49.40 AFV.select :holdingCoil
@22:35:49.51 ASV.dial :waste1
@22:35:49.89 ATV.dial :air3
@22:35:51.24 AS.reconfigure ASslow1
@22:35:51.28 AS.seek 0.51ml
@22:35:53.60 delay 2 seconds
@22:35:55.62 ATV.dial :bleach
@22:35:56.25 AS.seek 0.81ml
@22:36:56.82 delay 3 seconds
@22:36:59.85 ATV.dial :air3
@22:37:00.41 delay 3 seconds
@22:37:03.48 AS.seek 0.835ml
@22:37:08.83 delay 3 seconds
@22:37:11.86 ATV.dial :bleach
@22:37:12.49 AS.seek 0.82ml
@22:37:15.80 delay 3 seconds
@22:37:18.83 ARV.dial :waste
@22:37:19.44 delay 3 seconds
@22:37:22.47 ATV.dial :ARV
@22:37:23.39 AS.reconfigure ASconfig
@22:37:23.43 AS.seek 0.47ml
@22:37:43.85 AFV.select :carrierSolution
@22:37:43.96 delay 2 seconds
@22:37:45.98 AS.seek 1ml
@22:38:16.69 delay 1 second
@22:38:17.71 AFV.select :holdingCoil
@22:38:17.82 delay 1 second
@22:38:18.84 AS.reconfigure ASfast
@22:38:18.89 AS.seek 0ml
@22:38:46.21 AFV.select :carrierSolution
@22:38:46.32 delay 2 seconds
@22:38:48.34 AS.reconfigure ASconfig
@22:38:48.39 AS.seek 1ml
@22:39:46.11 delay 1 second
@22:39:47.13 AFV.select :holdingCoil
@22:39:47.24 delay 1 second
@22:39:48.26 AS.reconfigure ASfast
@22:39:48.31 AS.seek 0ml
@22:40:15.60 AFV.select :carrierSolution
@22:40:15.71 delay 2 seconds
@22:40:17.73 AS.reconfigure ASconfig
@22:40:17.78 AS.seek 1ml
@22:41:15.52 delay 1 second
@22:41:16.54 AFV.select :holdingCoil
@22:41:16.65 delay 1 second
@22:41:17.67 AS.reconfigure ASfast
@22:41:17.72 AS.seek 0ml
@22:41:45.03 ARV.dial between carrier and air1
@22:41:45.55 ATV.dial :air3
@22:41:47.31 AS.reconfigure ASslow1
@22:41:47.35 AS.seek 0.01ml
@22:41:49.63 delay 2 seconds
@22:41:51.66 ATV.dial :bleach
@22:41:52.30 AS.seek 0.81ml
@22:44:33.52 delay 3 seconds
@22:44:36.55 ATV.dial :air3
@22:44:37.11 delay 3 seconds
@22:44:40.18 AS.seek 0.835ml
@22:44:45.56 delay 3 seconds
@22:44:48.59 ATV.dial :bleach
@22:44:49.22 AS.seek 0.82ml
@22:44:52.50 delay 3 seconds
@22:44:55.53 ATV.dial PCR
@22:44:55.95 AS.reconfigure ASconfig
@22:44:55.99 AS.seek 0ml
@22:45:43.39 PCR.program :bleachProfile
@22:45:43.46 PCR program should complete in < 2:45
@22:45:43.47 PCR.run 10:00
@22:48:39.25 PCR.off
@22:48:39.27 AFV.select :carrierSolution
@22:48:39.38 delay 2 seconds
@22:48:41.40 AS.seek 1ml
@22:49:39.14 delay 1 second
@22:49:40.16 AFV.select :holdingCoil
@22:49:40.27 delay 1 second
@22:49:41.29 AS.reconfigure ASfast
@22:49:41.34 AS.seek 0ml
@22:50:08.66 delay 3 seconds
@22:50:11.68 AFV.select :carrierSolution
@22:50:11.79 delay 2 seconds
@22:50:13.81 AS.reconfigure ASconfig
@22:50:13.86 AS.seek 1ml
@22:51:11.60 delay 1 second
@22:51:12.62 AFV.select :holdingCoil
@22:51:12.73 delay 1 second
@22:51:13.75 AS.reconfigure ASfast
@22:51:13.80 AS.seek 0ml
@22:51:41.13 delay 3 seconds
@22:51:44.15 AFV.select :carrierSolution
@22:51:44.26 delay 2 seconds
@22:51:46.28 AS.reconfigure ASconfig
@22:51:46.33 AS.seek 1ml
@22:52:44.11 delay 1 second
@22:52:45.13 AFV.select :holdingCoil
@22:52:45.24 delay 1 second
@22:52:46.26 AS.reconfigure ASfast
@22:52:46.31 AS.seek 0ml
@22:53:13.63 delay 3 seconds
@22:53:16.65 AFV.select :carrierSolution
@22:53:16.76 delay 2 seconds
@22:53:18.78 AS.reconfigure ASconfig
@22:53:18.84 AS.seek 1ml
@22:54:16.58 delay 1 second
@22:54:17.60 AFV.select :holdingCoil
@22:54:17.71 delay 1 second
@22:54:18.73 AS.reconfigure ASfast
@22:54:18.78 AS.seek 0ml
@22:54:46.11 delay 3 seconds
@22:54:49.13 AFV.select :carrierSolution
@22:54:49.24 delay 2 seconds
@22:54:51.26 AS.reconfigure ASconfig
@22:54:51.31 AS.seek 1ml
@22:55:49.05 delay 1 second
@22:55:50.07 AFV.select :holdingCoil
@22:55:50.18 delay 1 second
@22:55:51.20 AS.reconfigure ASnorm
@22:55:51.25 AS.seek 0ml
@22:56:42.34 Setting up PCR for AIPc -- writing /var/log/esp/AIPc21mar2522h.pcr
@22:56:42.35 Building PCR Slug from ASV with MM3 mix, PP6 primer
@22:56:42.36 Priming with carrier
@22:56:42.37 ATV.dial ARV
@22:56:42.99 ARV.dial :carrier
@22:56:43.33 AS.seek 0.5ml
@22:57:08.89 AFV.select :carrierSolution
@22:57:09.00 delay 2 seconds
@22:57:11.02 AS.reconfigure ASconfig
@22:57:11.07 AS.seek 1ml
@22:57:40.11 delay 1 second
@22:57:41.14 AFV.select :holdingCoil
@22:57:41.24 delay 1 second
@22:57:42.26 ATV.dial :waste
@22:57:44.03 AS.reconfigure ASnorm
@22:57:44.07 AS.seek 0ml
@22:58:34.77 AFV.select :carrierSolution
@22:58:34.88 delay 2 seconds
@22:58:36.90 AS.reconfigure ASconfig
@22:58:36.95 AS.seek 0.5ml
@22:59:05.97 delay 1 second
@22:59:07.00 AFV.select :holdingCoil
@22:59:07.10 Building Slug
@22:59:07.11 ATV.dial :ARV
@22:59:08.83 delay 2 seconds
@22:59:10.86 ARV.dial :air1
@22:59:11.24 delay 2 seconds
@22:59:13.31 AS.reconfigure ASslow2
@22:59:13.36 AS.seek 0.506ml
@22:59:19.61 delay 2 seconds
@22:59:21.64 ARV.dial :MM3
@22:59:22.72 delay 2 seconds
@22:59:24.75 Adding MM3
@22:59:24.82 AS.seek 0.524ml
@22:59:43.16 delay 25 seconds
@23:00:08.19 ARV.dial :PP6
@23:00:08.58 Adding PP6
@23:00:08.59 delay 2 seconds
@23:00:10.66 AS.seek 0.53ml
@23:00:16.99 delay 10 seconds
@23:00:27.03 ARV.dial :air1
@23:00:27.89 adding ARV air
@23:00:27.90 delay 2 seconds
@23:00:29.96 AS.seek 0.536ml
@23:00:36.44 delay 5 seconds
@23:00:41.47 ARV.dial :carrier
@23:00:41.88 delay 7 seconds
@23:00:48.96 AS.seek 0.594ml
@23:01:47.74 delay 5 seconds
@23:01:52.77 ATV.dial :ASV
@23:01:53.18 delay 2 seconds
@23:01:55.25 AS.seek 0.6ml
@23:02:01.43 delay 4 seconds
@23:02:05.46 ATV.dial ARV
@23:02:06.04 delay 2 seconds
@23:02:08.07 ARV.dial :carrier
@23:02:08.08 delay 2 seconds
@23:02:10.14 AS.seek 0.65ml
@23:03:00.60 delay 4 seconds
@23:03:04.63 ATV.dial PCR
@23:03:05.24 ASV.dial :waste1
@23:03:05.26 ARV.dial :air1
@23:03:05.65 Positioning Slug by 0.324ml
@23:03:05.72 AS.seek 0.326ml
@23:08:32.11 delay 5 seconds
@23:08:37.14 ATV.dial ARV
@23:08:37.94 ASV.dial :PCRlock
@23:08:38.33 delay 5 seconds
@23:08:43.36 Running AIPc ProfileIPc thermocycling
@23:08:43.37 <PCR> PCR.program ProfileIPc
@23:08:43.47 ProfileIPc should complete in < 1:26:35
@23:08:43.48 PCR.run 1:53:13.75
@00:17:09.06CET26-Mar-21 PCR.off
@00:17:09.61 <MFBPCR> PCR for AIPc completed -- closed /var/log/esp/AIPc21mar2522h.pcr
@00:17:09.62 Full MFB Decontamination
@00:17:09.63 Decon extraction column and decon extSrc
@00:17:09.65 SPE.hold 2:46:40,55C
@00:17:09.68 ASV.dial :SPEdelivery
@00:17:10.36 delay 20 seconds
@00:17:30.39 AFV.select :carrierSolution
@00:17:30.50 delay 2 seconds
@00:17:32.52 AS.reconfigure ASconfig
@00:17:32.57 AS.seek 0.5ml
@00:17:42.88 delay 1 second
@00:17:43.90 AFV.select :holdingCoil
@00:17:44.01 ATV.dial :air1
@00:17:46.27 AS.reconfigure ASslow1
@00:17:46.31 AS.seek 0.52ml
@00:17:50.61 ATV.dial :ASV
@00:17:52.36 ASV.dial :SPErecovery
@00:17:53.05 AS.seek 0.62ml
@00:18:13.43 ATV.dial :waste
@00:18:15.62 AS.reconfigure ASnorm
@00:18:15.66 AS.seek 0ml
@00:18:47.27 deconEluateRecoveryLine extSrc
@00:18:47.28 ATV.dial :waste
@00:18:47.30 AS.seek 0ml
@00:18:47.36 ATV.dial :air3
@00:18:48.11 AS.reconfigure ASslow1
@00:18:48.15 AS.seek 0.01ml
@00:18:50.51 delay 2 seconds
@00:18:52.54 ATV.dial :bleach
@00:18:53.03 AS.seek 0.31ml
@00:19:53.63 delay 3 seconds
@00:19:56.66 ATV.dial :air3
@00:19:57.27 delay 3 seconds
@00:20:00.34 AS.seek 0.335ml
@00:20:05.62 delay 3 seconds
@00:20:08.65 ATV.dial :bleach
@00:20:09.32 AS.seek 0.32ml
@00:20:12.68 delay 3 seconds
@00:20:15.71 ATV.dial :extSrc
@00:20:16.37 AS.seek 0ml
@00:21:21.01 AFV.select :carrierSolution
@00:21:21.12 delay 2 seconds
@00:21:23.14 AS.reconfigure ASconfig
@00:21:23.19 AS.seek 0.5ml
@00:21:52.21 delay 1 second
@00:21:53.23 AFV.select :holdingCoil
@00:21:53.66 ATV.dial :extSrc
@00:21:53.69 AS.reconfigure ASslow1
@00:21:53.74 AS.seek 0ml
@00:23:34.57 AFV.select :carrierSolution
@00:23:34.68 delay 2 seconds
@00:23:36.70 AS.reconfigure ASconfig
@00:23:36.75 AS.seek 0.5ml
@00:24:05.77 delay 1 second
@00:24:06.79 AFV.select :holdingCoil
@00:24:06.90 ATV.dial :extSrc
@00:24:06.92 AS.reconfigure ASslow1
@00:24:06.96 AS.seek 0ml
@00:25:47.77 AFV.select :carrierSolution
@00:25:47.88 delay 2 seconds
@00:25:49.90 AS.reconfigure ASconfig
@00:25:49.95 AS.seek 0.5ml
@00:26:18.97 delay 1 second
@00:26:20.00 AFV.select :holdingCoil
@00:26:20.10 ATV.dial :extSrc
@00:26:20.12 AS.reconfigure ASslow1
@00:26:20.16 AS.seek 0ml
@00:28:00.97 AFV.select :carrierSolution
@00:28:01.08 delay 2 seconds
@00:28:03.10 AS.reconfigure ASconfig
@00:28:03.15 AS.seek 0.5ml
@00:28:32.18 delay 1 second
@00:28:33.20 AFV.select :holdingCoil
@00:28:33.31 ATV.dial :extSrc
@00:28:33.33 AS.reconfigure ASslow1
@00:28:33.37 AS.seek 0ml
@00:30:14.16 ATV.dial :air2
@00:30:15.55 AS.reconfigure ASslow0
@00:30:15.59 AS.seek 0.5ml
@00:31:06.22 ATV.dial :extSrc
@00:31:07.80 AS.seek 0.02ml
@00:31:56.41 delay 1 second
@00:31:57.44 ATV.dial :waste
@00:31:58.80 AS.reconfigure ASconfig
@00:31:58.84 AS.seek 0ml
@00:32:00.27 AFV.select :holdingCoil
@00:32:00.28 ATV.dial :waste
@00:32:00.30 AS.seek 0ml
@00:32:00.36 AFV.select :carrierSolution
@00:32:00.46 delay 2 seconds
@00:32:02.48 AS.seek 0.5ml
@00:32:31.49 delay 1 second
@00:32:32.51 AFV.select :holdingCoil
@00:32:32.62 ATV.dial :air3
@00:32:33.49 AS.reconfigure ASslow1
@00:32:33.53 AS.seek 0.52ml
@00:32:37.82 ATV.dial :air3
@00:32:37.89 AS.seek 0.53ml
@00:32:40.18 delay 2 seconds
@00:32:42.21 ATV.dial :bleach
@00:32:42.67 AS.seek 0.71ml
@00:33:19.19 delay 3 seconds
@00:33:22.22 ATV.dial :air3
@00:33:22.80 delay 3 seconds
@00:33:25.87 AS.seek 0.735ml
@00:33:31.13 delay 3 seconds
@00:33:34.16 ATV.dial :bleach
@00:33:34.78 AS.seek 0.72ml
@00:33:38.18 delay 3 seconds
@00:33:41.21 ATV.dial ASV
@00:33:42.30 ASV.dial :SPErecovery
@00:33:42.37 AS.seek 0.365ml
@00:34:54.02 ATV.dial :waste
@00:34:55.84 AS.reconfigure ASnorm
@00:34:55.88 AS.seek 0ml
@00:35:14.61 ATV.dial :air3
@00:35:15.32 AS.reconfigure ASslow1
@00:35:15.36 AS.seek 0.01ml
@00:35:17.71 delay 2 seconds
@00:35:19.74 ATV.dial :bleach
@00:35:20.21 AS.seek 0.58ml
@00:37:15.16 delay 3 seconds
@00:37:18.19 ATV.dial :air3
@00:37:18.77 delay 3 seconds
@00:37:21.84 AS.seek 0.605ml
@00:37:27.21 delay 3 seconds
@00:37:30.24 ATV.dial :bleach
@00:37:30.86 AS.seek 0.59ml
@00:37:34.15 delay 3 seconds
@00:37:37.18 ATV.dial :ASV
@00:37:38.26 ASV.dial :wasteR
@00:37:38.66 AS.seek 0ml
@00:39:37.54 delay 1:00
@00:40:37.58 ATV.dial ASV
@00:40:37.59 AFV.select :carrierSolution
@00:40:37.69 delay 2 seconds
@00:40:39.71 AS.reconfigure ASconfig
@00:40:39.76 AS.seek 1ml
@00:41:37.52 delay 1 second
@00:41:38.54 AFV.select :holdingCoil
@00:41:38.65 delay 1 second
@00:41:39.67 ASV.dial :wasteR
@00:41:39.68 AS.reconfigure ASslow1
@00:41:39.73 AS.seek 0ml
@00:45:01.08 delay 10 seconds
@00:45:11.12 ATV.dial :air3
@00:45:12.72 AS.seek 0.01ml
@00:45:15.08 delay 2 seconds
@00:45:17.11 ATV.dial :bleach
@00:45:17.75 AS.seek 0.58ml
@00:47:12.65 delay 3 seconds
@00:47:15.68 ATV.dial :air3
@00:47:16.26 delay 3 seconds
@00:47:19.34 AS.seek 0.605ml
@00:47:24.69 delay 3 seconds
@00:47:27.72 ATV.dial :bleach
@00:47:28.35 AS.seek 0.59ml
@00:47:31.97 delay 3 seconds
@00:47:35.00 ATV.dial ASV
@00:47:36.08 ASV.dial :SPEdelivery
@00:47:36.47 AS.seek 0ml
@00:49:35.33 delay 1:00
@00:50:35.36 AFV.select :carrierSolution
@00:50:35.47 delay 2 seconds
@00:50:37.49 AS.reconfigure ASconfig
@00:50:37.54 AS.seek 1ml
@00:51:35.33 delay 1 second
@00:51:36.35 AFV.select :holdingCoil
@00:51:36.46 delay 1 second
@00:51:37.48 ATV.dial ASV
@00:51:37.49 ASV.dial :SPEdelivery
@00:51:37.50 AS.reconfigure ASslow1
@00:51:37.55 AS.seek 0ml
@00:54:58.93 delay 10 seconds
@00:55:08.97 ATV.dial :air2
@00:55:09.83 AS.reconfigure ASslow0
@00:55:09.87 AS.seek 0.8ml
@00:56:30.63 delay 3 seconds
@00:56:33.66 ATV.dial :ASV
@00:56:34.69 ASV.dial :SPEdelivery
@00:56:34.71 AS.reconfigure ASslow1
@00:56:34.75 AS.seek 0.03ml
@00:59:09.90 delay 20 seconds
@00:59:29.94 ATV.dial :waste,via: :air3
@00:59:31.78 AS.reconfigure ASconfig
@00:59:31.82 AS.seek 0ml
@00:59:33.82 SPE.coast
@00:59:33.89 Removing Bleach from Mixing Coil
@00:59:33.90 AFV.select :carrierSolution
@00:59:34.01 delay 2 seconds
@00:59:36.03 AS.seek 0.5ml
@01:00:05.02 delay 1 second
@01:00:06.04 AFV.select :holdingCoil
@01:00:06.15 ATV.dial :ASV
@01:00:07.96 ASV.dial :SPErecovery
@01:00:08.61 AS.reconfigure ASslow1
@01:00:08.65 AS.seek 0.73ml
@01:00:55.15 ASV.dial :wasteD
@01:00:55.75 AS.reconfigure ASnorm
@01:00:55.79 AS.seek 0ml
@01:01:32.90 AFV.select :carrierSolution
@01:01:33.01 delay 2 seconds
@01:01:35.03 AS.reconfigure ASconfig
@01:01:35.08 AS.seek 0.5ml
@01:02:04.12 delay 1 second
@01:02:05.14 AFV.select :holdingCoil
@01:02:05.25 ATV.dial ASV
@01:02:05.26 ASV.dial :SPErecovery
@01:02:05.91 AS.reconfigure ASslow0
@01:02:05.95 AS.seek 0.3ml
@01:02:26.35 ATV.dial :air2
@01:02:27.44 AS.reconfigure ASslow1
@01:02:27.48 AS.seek 0.32ml
@01:02:31.83 ATV.dial ASV
@01:02:32.93 AS.reconfigure ASslow0
@01:02:32.97 AS.seek 0.57ml
@01:02:58.46 ASV.dial :wasteD
@01:02:59.06 AS.reconfigure ASnorm
@01:02:59.10 AS.seek 0ml
@01:03:28.12 AFV.select :carrierSolution
@01:03:28.23 delay 2 seconds
@01:03:30.25 AS.reconfigure ASconfig
@01:03:30.30 AS.seek 0.5ml
@01:03:59.30 delay 1 second
@01:04:00.32 AFV.select :holdingCoil
@01:04:00.43 ATV.dial ASV
@01:04:00.44 ASV.dial :SPErecovery
@01:04:01.08 AS.reconfigure ASslow0
@01:04:01.12 AS.seek 0.3ml
@01:04:21.54 ATV.dial :air2
@01:04:22.63 AS.reconfigure ASslow1
@01:04:22.67 AS.seek 0.32ml
@01:04:27.13 ATV.dial ASV
@01:04:28.22 AS.reconfigure ASslow0
@01:04:28.26 AS.seek 0.57ml
@01:04:53.76 ASV.dial :wasteD
@01:04:54.36 AS.reconfigure ASnorm
@01:04:54.40 AS.seek 0ml
@01:05:23.46 ATV.dial :air2
@01:05:24.50 AS.reconfigure ASslow0
@01:05:24.54 AS.seek 0.3ml
@01:05:55.12 ATV.dial :ASV
@01:05:56.15 ASV.dial :wasteD
@01:05:56.17 AS.reconfigure ASconfig
@01:05:56.21 AS.seek 0.05ml
@01:06:10.88 ATV.dial :waste
@01:06:12.88 AS.seek 0ml
@01:06:16.03 ASV.dial :SPEdelivery
@01:06:17.11 Cleaning PCR
@01:06:17.12 AFV.select :carrierSolution
@01:06:17.22 delay 2 seconds
@01:06:19.24 AS.seek 0.5ml
@01:06:48.27 delay 1 second
@01:06:49.29 AFV.select :holdingCoil
@01:06:49.40 ASV.dial :waste1
@01:06:49.77 ATV.dial :air3
@01:06:50.47 AS.reconfigure ASslow1
@01:06:50.51 AS.seek 0.51ml
@01:06:52.90 delay 2 seconds
@01:06:54.93 ATV.dial :bleach
@01:06:55.38 AS.seek 0.81ml
@01:07:56.10 delay 3 seconds
@01:07:59.13 ATV.dial :air3
@01:07:59.69 delay 3 seconds
@01:08:02.76 AS.seek 0.835ml
@01:08:08.51 delay 3 seconds
@01:08:11.54 ATV.dial :bleach
@01:08:12.14 AS.seek 0.82ml
@01:08:15.44 delay 3 seconds
@01:08:18.47 ARV.dial :waste
@01:08:19.08 delay 3 seconds
@01:08:22.11 ATV.dial :ARV
@01:08:23.02 AS.reconfigure ASconfig
@01:08:23.06 AS.seek 0.47ml
@01:08:43.46 AFV.select :carrierSolution
@01:08:43.57 delay 2 seconds
@01:08:45.59 AS.seek 1ml
@01:09:16.32 delay 1 second
@01:09:17.34 AFV.select :holdingCoil
@01:09:17.45 delay 1 second
@01:09:18.47 AS.reconfigure ASfast
@01:09:18.52 AS.seek 0ml
@01:09:45.83 AFV.select :carrierSolution
@01:09:45.94 delay 2 seconds
@01:09:47.96 AS.reconfigure ASconfig
@01:09:48.01 AS.seek 1ml
@01:10:45.79 delay 1 second
@01:10:46.81 AFV.select :holdingCoil
@01:10:46.92 delay 1 second
@01:10:47.94 AS.reconfigure ASfast
@01:10:47.99 AS.seek 0ml
@01:11:15.32 AFV.select :carrierSolution
@01:11:15.43 delay 2 seconds
@01:11:17.45 AS.reconfigure ASconfig
@01:11:17.50 AS.seek 1ml
@01:12:15.22 delay 1 second
@01:12:16.24 AFV.select :holdingCoil
@01:12:16.35 delay 1 second
@01:12:17.37 AS.reconfigure ASfast
@01:12:17.42 AS.seek 0ml
@01:12:44.76 ARV.dial between carrier and air1
@01:12:45.28 ATV.dial :air3
@01:12:46.61 AS.reconfigure ASslow1
@01:12:46.65 AS.seek 0.01ml
@01:12:49.04 delay 2 seconds
@01:12:51.07 ATV.dial :bleach
@01:12:51.69 AS.seek 0.81ml
@01:15:32.83 delay 3 seconds
@01:15:35.86 ATV.dial :air3
@01:15:36.43 delay 3 seconds
@01:15:39.51 AS.seek 0.835ml
@01:15:44.80 delay 3 seconds
@01:15:47.83 ATV.dial :bleach
@01:15:48.46 AS.seek 0.82ml
@01:15:51.80 delay 3 seconds
@01:15:54.83 ATV.dial PCR
@01:15:55.23 AS.reconfigure ASconfig
@01:15:55.27 AS.seek 0ml
@01:16:42.66 PCR.program :bleachProfile
@01:16:42.73 PCR program should complete in < 2:45
@01:16:42.74 PCR.run 10:00
@01:19:44.96 PCR.off
@01:19:45.01 AFV.select :carrierSolution
@01:19:45.11 delay 2 seconds
@01:19:47.13 AS.seek 1ml
@01:20:44.91 delay 1 second
@01:20:45.93 AFV.select :holdingCoil
@01:20:46.04 delay 1 second
@01:20:47.06 AS.reconfigure ASfast
@01:20:47.11 AS.seek 0ml
@01:21:14.41 delay 3 seconds
@01:21:17.44 AFV.select :carrierSolution
@01:21:17.54 delay 2 seconds
@01:21:19.56 AS.reconfigure ASconfig
@01:21:19.61 AS.seek 1ml
@01:22:17.38 delay 1 second
@01:22:18.40 AFV.select :holdingCoil
@01:22:18.51 delay 1 second
@01:22:19.53 AS.reconfigure ASfast
@01:22:19.58 AS.seek 0ml
@01:22:46.91 delay 3 seconds
@01:22:49.93 AFV.select :carrierSolution
@01:22:50.04 delay 2 seconds
@01:22:52.06 AS.reconfigure ASconfig
@01:22:52.11 AS.seek 1ml
@01:23:49.88 delay 1 second
@01:23:50.90 AFV.select :holdingCoil
@01:23:51.01 delay 1 second
@01:23:52.03 AS.reconfigure ASfast
@01:23:52.08 AS.seek 0ml
@01:24:19.40 delay 3 seconds
@01:24:22.42 AFV.select :carrierSolution
@01:24:22.53 delay 2 seconds
@01:24:24.55 AS.reconfigure ASconfig
@01:24:24.60 AS.seek 1ml
@01:25:22.37 delay 1 second
@01:25:23.39 AFV.select :holdingCoil
@01:25:23.50 delay 1 second
@01:25:24.52 AS.reconfigure ASfast
@01:25:24.57 AS.seek 0ml
@01:25:51.90 delay 3 seconds
@01:25:54.92 AFV.select :carrierSolution
@01:25:55.03 delay 2 seconds
@01:25:57.05 AS.reconfigure ASconfig
@01:25:57.10 AS.seek 1ml
@01:26:54.88 delay 1 second
@01:26:55.90 AFV.select :holdingCoil
@01:26:56.01 delay 1 second
@01:26:57.03 AS.reconfigure ASnorm
@01:26:57.08 AS.seek 0ml
@01:27:47.78 AFV.select :carrierSolution
@01:27:47.89 delay 2 seconds
@01:27:49.91 AS.reconfigure ASconfig
@01:27:49.96 AS.seek 1ml
@01:28:47.74 delay 1 second
@01:28:48.76 AFV.select :holdingCoil
@01:28:48.87 delay 1 second
@01:28:49.89 ATV.dial :waste
@01:28:51.17 AS.reconfigure ASnorm
@01:28:51.21 AS.seek 0.5ml
@01:29:16.76 ATV.dial :waste2
@01:29:18.11 AS.seek 0ml
@11:40:40.43CET26-Mar-21 <MAIN> ESP2 v3.30 I2C Gateway v3.06 for core bus
@11:40:42.56 
Configuration for mer 7/20/20 brent@mbari.org
/home/esp/esp2/type/shallow/mer/configure.rb
@11:40:42.65 
Configuration for MFB3 by preston@mbari.org
/home/esp/esp2/type/mfb/MFB3/configure.rb
@11:40:47.01 Polling Can every 10:00
@11:40:48.72 CS.configure :default
@11:40:48.76 CS.hold
@11:40:48.82 CS.configure :default
@11:40:48.90 PS.configure :default
@11:40:48.98 SS.configure :default
@11:40:49.02 SS.hold
@11:40:49.07 SS.configure :default
@11:40:49.15 CC.configure :default
@11:40:49.18 CC.hold
@11:40:49.23 CC.configure :default
@11:40:49.31 PC.configure :default
@11:40:49.35 PC.hold
@11:40:49.40 PC.configure :default
@11:40:49.50 Forearm.configure :loose
@11:40:49.53 Forearm.hold
@11:40:49.59 Forearm.configure :loose
@11:40:49.67 Elbow.configure :loose
@11:40:49.71 Elbow.hold
@11:40:49.76 Elbow.configure :loose
@11:40:49.86 SC.configure :loose
@11:40:49.90 SC.hold
@11:40:49.95 SC.configure :loose
@11:40:50.03 SE.configure :default
@11:40:50.07 SE.hold
@11:40:50.12 SE.configure :default
@11:40:50.16 CH.coast
@11:40:50.20 CH.configure :default
@11:40:50.23 PH.coast
@11:40:50.28 PH.configure :default
@11:40:50.38 SPE.coast
@11:40:50.42 SPE.configure SPEconfig
@11:40:50.50 AS.configure ASconfig
@11:40:50.54 AS.hold
@11:40:50.58 AS.configure ASconfig
@11:40:50.62 PCR.off
@11:40:50.65 PCR.pidtemp= 50,0.1,0,300,25,25
@11:40:50.69 PCR.caltemp= 2.5051,0.0372,0
@11:40:50.72 All dwarf controllers running firmware version 3.75
@11:40:50.90 Cleanly exited at 11:39:22CET26-Mar-21
@11:40:50.93 Loading /home/esp/esp2/mission/GENOMAP.rb mission
@11:40:51.07 <MFBPCR> Extracting Nucleic Acid from extSrc
@11:40:51.10 SPE.hold 2:46:40,55C
@11:40:51.15 ASV.dial :SPEdelivery
@11:40:51.57 delay 20 seconds
@11:40:53.41 <mwj> Session Begun
@11:41:11.60 <MFBPCR> AFV.select :holdingCoil
@11:41:11.73 ATV.dial :air2
@11:41:13.21 AS.reconfigure ASslow1
@11:41:13.27 AS.seek 0.07ml
@11:41:27.65 delay 3 seconds
@11:41:30.68 ATV.dial :extSrc
@11:41:32.21 AS.seek 0.47ml
@11:42:52.93 delay 5 seconds
@11:42:58.01 Solid Phase Extraction Heater at 39C
@11:42:58.02 ATV.dial ASV
@11:42:58.91 ASV.dial :SPEdelivery
@11:42:59.00 AS.seek 0.042ml
@11:44:25.36 delay 10 seconds
@11:44:35.40 Rinsing external lysate line extSrc
@11:44:35.41 AFV.select :carrierSolution
@11:44:35.53 delay 2 seconds
@11:44:37.55 AS.reconfigure ASconfig
@11:44:37.61 AS.seek 1ml
@11:45:32.97 delay 1 second
@11:45:33.99 AFV.select :holdingCoil
@11:45:34.10 delay 1 second
@11:45:35.12 ATV.dial :extSrc
@11:45:35.95 AS.reconfigure ASnorm
@11:45:36.00 AS.seek 0ml
@11:46:26.68 ATV.dial :air2
@11:46:28.27 AS.reconfigure ASslow0
@11:46:28.33 AS.seek 0.05ml
@11:46:33.72 ATV.dial :extSrc
@11:46:35.30 AS.seek 0.01ml
@11:46:39.65 ATV.dial :waste
@11:46:41.05 AS.reconfigure ASconfig
@11:46:41.10 AS.seek 0ml
@11:46:42.35 ethanol wash
@11:46:42.36 ATV.dial :air2
@11:46:43.53 AS.reconfigure ASslow2
@11:46:43.59 AS.seek 0.01ml
@11:46:54.61 delay 3 seconds
@11:46:57.64 Pulling 0.4ml of Ethanol Wash
@11:46:57.65 ATV.dial :ethanol
@11:46:58.53 AS.reconfigure ASslow1
@11:46:58.59 AS.seek 0.41ml
@11:48:19.40 delay 3 seconds
@11:48:22.43 ATV.dial :air1
@11:48:22.85 delay 3 seconds
@11:48:25.92 AS.seek 0.435ml
@11:48:31.24 delay 3 seconds
@11:48:34.27 ATV.dial :ethanol
@11:48:34.91 AS.seek 0.42ml
@11:48:38.24 delay 3 seconds
@11:48:41.27 ATV.dial ASV
@11:48:42.66 AS.seek 0.093ml
@11:49:48.74 delay 3 seconds
@11:49:51.77 ATV.dial :waste2
@11:49:52.58 AS.reconfigure ASconfig
@11:49:52.63 AS.seek 0ml
@11:49:58.30 ATV.dial :air2
@11:49:58.72 AS.reconfigure ASslow1
@11:49:58.77 AS.seek 1ml
@11:53:20.13 delay 5 seconds
@11:53:25.16 ATV.dial ASV
@11:53:26.21 ASV.dial :SPEdelivery
@11:53:26.24 AS.seek 0.02ml
@11:56:43.65 delay 5 seconds
@11:56:48.68 ATV.dial :air2
@11:56:49.78 AS.seek 0.52ml
@11:58:30.61 delay 5 seconds
@11:58:35.64 ATV.dial ASV
@11:58:36.68 ASV.dial :wasteR
@11:58:37.08 AS.seek 0.3ml
@11:59:21.61 delay 20 seconds
@11:59:41.65 AS.seek 0.07ml
@12:00:28.19 delay 20 seconds
@12:00:48.22 ATV.dial :waste2
@12:00:49.03 AS.reconfigure ASconfig
@12:00:49.08 AS.seek 0ml
@12:00:53.40 Elution
@12:00:53.41 ATV.dial :air1
@12:00:54.60 AS.reconfigure ASslow1
@12:00:54.65 AS.seek 0.05ml
@12:01:05.05 delay 3 seconds
@12:01:08.08 ATV.dial :ElutionWater
@12:01:08.80 AS.seek 0.11ml
@12:01:21.15 delay 3 seconds
@12:01:24.18 ATV.dial ASV,via: :air2
@12:01:26.42 ASV.dial :SPEelution
@12:01:27.04 AFV.select :carrierSolution
@12:01:27.20 AS.reconfigure ASnorm
@12:01:27.26 AS.seek 0.76ml
@12:02:00.32 delay 3 seconds
@12:02:03.34 AFV.select :holdingCoil
@12:02:03.49 AS.reconfigure ASslow1
@12:02:03.55 AS.seek 0.475ml
@12:03:01.15 delay 5 seconds
@12:03:06.23 AS.reconfigure ASslow2
@12:03:06.29 AS.seek 0.44ml
@12:03:41.79 delay 1:00
@12:04:41.83 pushing eluate to holding coil
@12:04:41.90 AS.seek 0.28ml
@12:07:23.05 delay 30 seconds
@12:07:53.09 ATV.dial :waste,via: :air3
@12:07:54.95 AS.reconfigure ASconfig
@12:07:55.00 AS.seek 0ml
@12:08:11.41 Parking Bleach in LysateSource line and SPEcolumn
@12:08:11.43 ATV.dial :air3
@12:08:12.33 AS.reconfigure ASslow1
@12:08:12.38 AS.seek 0.03ml
@12:08:18.75 ATV.dial :ElutionWater
@12:08:19.39 AS.seek 0.06ml
@12:08:25.69 ATV.dial :air3
@12:08:26.33 AS.seek 0.07ml
@12:08:28.61 delay 2 seconds
@12:08:30.64 ATV.dial :bleach
@12:08:31.13 AS.seek 0.15ml
@12:08:47.50 delay 3 seconds
@12:08:50.53 ATV.dial :air3
@12:08:51.13 delay 3 seconds
@12:08:54.20 AS.seek 0.175ml
@12:08:59.56 delay 3 seconds
@12:09:02.59 ATV.dial :bleach
@12:09:03.24 AS.seek 0.16ml
@12:09:06.52 delay 3 seconds
@12:09:09.55 ATV.dial :extSrc
@12:09:10.20 AS.reconfigure ASconfig
@12:09:10.25 AS.seek 0.015ml
@12:09:18.88 ATV.dial :waste
@12:09:20.45 AS.seek 0ml
@12:09:21.60 ATV.dial :air3
@12:09:22.50 AS.reconfigure ASslow1
@12:09:22.56 AS.seek 0.01ml
@12:09:24.88 delay 2 seconds
@12:09:26.91 ATV.dial :bleach
@12:09:27.39 AS.seek 0.58ml
@12:11:22.27 delay 3 seconds
@12:11:25.30 ATV.dial :air3
@12:11:25.90 delay 3 seconds
@12:11:28.97 AS.seek 0.605ml
@12:11:34.29 delay 3 seconds
@12:11:37.32 ATV.dial :bleach
@12:11:37.96 AS.seek 0.59ml
@12:11:41.22 delay 3 seconds
@12:11:44.25 ATV.dial :ASV
@12:11:45.33 ASV.dial :wasteR
@12:11:45.95 AS.seek 0ml
@12:13:44.86 delay 1:00
@12:14:44.89 AFV.select :carrierSolution
@12:14:45.00 delay 2 seconds
@12:14:47.02 AS.reconfigure ASconfig
@12:14:47.07 AS.seek 1ml
@12:15:44.85 delay 1 second
@12:15:45.87 AFV.select :holdingCoil
@12:15:45.98 delay 1 second
@12:15:47.00 ATV.dial ASV
@12:15:47.01 ASV.dial :wasteR
@12:15:47.03 AS.reconfigure ASslow1
@12:15:47.10 AS.seek 0ml
@12:19:08.58 delay 20 seconds
@12:19:28.62 ATV.dial :air3
@12:19:30.22 AS.seek 0.59ml
@12:21:29.15 delay 3 seconds
@12:21:32.18 ATV.dial :ASV
@12:21:33.69 ASV.dial :wasteR
@12:21:33.78 AS.seek 0.09ml
@12:23:14.65 delay 30 seconds
@12:23:44.69 ATV.dial :extSrc
@12:23:45.52 delay 3 seconds
@12:23:48.55 ATV.dial :ElutionWater
@12:23:49.75 AS.seek 0.12ml
@12:23:56.08 ATV.dial :air3
@12:23:56.74 AS.seek 0.13ml
@12:23:59.02 delay 2 seconds
@12:24:01.05 ATV.dial :bleach
@12:24:01.54 AS.seek 0.23ml
@12:24:21.97 delay 3 seconds
@12:24:25.00 ATV.dial :air3
@12:24:25.60 delay 3 seconds
@12:24:28.67 AS.seek 0.255ml
@12:24:34.00 delay 3 seconds
@12:24:37.03 ATV.dial :bleach
@12:24:37.67 AS.seek 0.24ml
@12:24:41.44 delay 3 seconds
@12:24:44.47 ATV.dial :ASV
@12:24:45.58 ASV.dial :SPEdelivery
@12:24:45.97 AFV.select :carrierSolution
@12:24:46.09 delay 2 seconds
@12:24:48.11 AS.reconfigure ASconfig
@12:24:48.16 AS.seek 0.5ml
@12:25:03.41 delay 1 second
@12:25:04.43 AFV.select :holdingCoil
@12:25:04.58 AS.reconfigure ASslow1
@12:25:04.64 AS.seek 0.17ml
@12:26:11.28 SPE.hold 2:46:40,95C
@12:26:11.34 delay 3:00
@12:29:11.39 AFV.select :carrierSolution
@12:29:11.50 delay 2 seconds
@12:29:13.52 AS.reconfigure ASconfig
@12:29:13.58 AS.seek 1ml
@12:30:01.58 delay 1 second
@12:30:02.60 AFV.select :holdingCoil
@12:30:02.71 delay 1 second
@12:30:03.73 ASV.dial :wasteD
@12:30:04.84 AS.seek 0ml
@12:31:02.61 Priming Eluate
@12:31:02.62 AFV.select :carrierSolution
@12:31:02.75 delay 2 seconds
@12:31:04.77 AS.seek 0.5ml
@12:31:33.80 delay 1 second
@12:31:34.82 AFV.select :holdingCoil
@12:31:34.93 ATV.dial ASV
@12:31:34.94 ASV.dial :SPErecovery
@12:31:35.57 Swish #1 of 2
@12:31:35.65 AS.reconfigure ASslow0
@12:31:35.71 AS.seek 0.6ml
@12:31:46.17 AS.seek 0.5ml
@12:31:56.55 Swish #2 of 2
@12:31:56.64 AS.seek 0.6ml
@12:32:07.11 AS.seek 0.5ml
@12:32:17.47 delay 3 seconds
@12:32:20.54 AS.reconfigure ASslow1
@12:32:20.60 AS.seek 0.684ml
@12:32:57.91 delay 3 seconds
@12:33:00.94 Eluate primed
@12:33:00.95 ATV.dial :waste2
@12:33:01.62 AS.reconfigure ASnorm
@12:33:01.67 AS.seek 0ml
@12:33:36.47 SPE.coast
@12:33:36.55 ASV.dial :SPErecovery
@12:33:36.58 Setting up PCR for ACYPP2 -- writing /var/log/esp/ACYPP221mar2612h.pcr
@12:33:36.60 Building PCR Slug from ASV with MM1 mix, PP2 primer
@12:33:36.61 Priming with carrier
@12:33:36.63 ATV.dial ARV
@12:33:37.69 ARV.dial :carrier
@12:33:38.03 AS.seek 0.5ml
@12:34:03.60 AFV.select :carrierSolution
@12:34:03.73 delay 2 seconds
@12:34:05.75 AS.reconfigure ASconfig
@12:34:05.82 AS.seek 1ml
@12:34:34.85 delay 1 second
@12:34:35.87 AFV.select :holdingCoil
@12:34:35.98 delay 1 second
@12:34:37.00 ATV.dial :waste
@12:34:38.62 AS.reconfigure ASnorm
@12:34:38.68 AS.seek 0ml
@12:35:29.36 AFV.select :carrierSolution
@12:35:29.49 delay 2 seconds
@12:35:31.51 AS.reconfigure ASconfig
@12:35:31.57 AS.seek 0.5ml
@12:36:00.60 delay 1 second
@12:36:01.62 AFV.select :holdingCoil
@12:36:01.73 Building Slug
@12:36:01.74 ATV.dial :ARV
@12:36:03.50 delay 2 seconds
@12:36:05.53 ARV.dial :air1
@12:36:05.94 delay 2 seconds
@12:36:08.01 AS.reconfigure ASslow2
@12:36:08.08 AS.seek 0.506ml
@12:36:14.30 delay 2 seconds
@12:36:16.33 ARV.dial :MM1
@12:36:16.96 delay 2 seconds
@12:36:18.99 Adding MM1
@12:36:19.06 AS.seek 0.524ml
@12:36:37.33 delay 25 seconds
@12:37:02.36 ARV.dial :PP2
@12:37:02.78 Adding PP2
@12:37:02.79 delay 2 seconds
@12:37:04.87 AS.seek 0.53ml
@12:37:11.07 delay 10 seconds
@12:37:21.11 ARV.dial :air2
@12:37:21.53 adding ARV air
@12:37:21.54 delay 2 seconds
@12:37:23.62 AS.seek 0.536ml
@12:37:29.79 delay 5 seconds
@12:37:34.82 ARV.dial :carrier
@12:37:36.16 delay 7 seconds
@12:37:43.24 AS.seek 0.594ml
@12:38:41.86 delay 5 seconds
@12:38:46.89 ATV.dial :ASV
@12:38:47.32 delay 2 seconds
@12:38:49.40 AS.seek 0.6ml
@12:38:55.81 delay 4 seconds
@12:38:59.83 ATV.dial ARV
@12:39:00.41 delay 2 seconds
@12:39:02.44 ARV.dial :carrier
@12:39:02.45 delay 2 seconds
@12:39:04.53 AS.seek 0.65ml
@12:39:55.25 delay 4 seconds
@12:39:59.28 ATV.dial PCR
@12:39:59.93 ASV.dial :waste1
@12:40:00.75 ARV.dial :air1
@12:40:01.17 Positioning Slug by 0.324ml
@12:40:01.25 AS.seek 0.326ml
@12:45:27.24 delay 5 seconds
@12:45:32.27 ATV.dial ARV
@12:45:33.08 ASV.dial :PCRlock
@12:45:33.47 delay 5 seconds
@12:45:38.50 Running ACYPP2 Profile60CIQ2 thermocycling
@12:45:38.51 <PCR> PCR.program Profile60CIQ2
@12:45:38.64 Profile60CIQ2 should complete in < 1:47:50
@12:45:38.65 PCR.run 2:19:47.5
@14:14:13.76 PCR.off
@14:14:14.76 <MFBPCR> Running ACYPP2 MeltProfile60 thermocycling
@14:14:14.77 <PCR> PCR.program MeltProfile60
@14:14:14.88 MeltProfile60 should complete in < 11:55
@14:14:14.89 PCR.run 19:53.75
@14:21:52.86 PCR.off
@14:21:53.79 <MFBPCR> PCR for ACYPP2 completed -- closed /var/log/esp/ACYPP221mar2612h.pcr
@14:21:53.86 Cleaning PCR
@14:21:53.87 AFV.select :carrierSolution
@14:21:53.99 delay 2 seconds
@14:21:56.01 AS.reconfigure ASconfig
@14:21:56.07 AS.seek 0.5ml
@14:22:06.38 delay 1 second
@14:22:07.40 AFV.select :holdingCoil
@14:22:07.51 ASV.dial :waste1
@14:22:07.91 ATV.dial :air3
@14:22:09.28 AS.reconfigure ASslow1
@14:22:09.34 AS.seek 0.51ml
@14:22:11.73 delay 2 seconds
@14:22:13.76 ATV.dial :bleach
@14:22:14.39 AS.seek 0.81ml
@14:23:15.08 delay 3 seconds
@14:23:18.11 ATV.dial :air3
@14:23:18.69 delay 3 seconds
@14:23:21.76 AS.seek 0.835ml
@14:23:27.07 delay 3 seconds
@14:23:30.10 ATV.dial :bleach
@14:23:30.74 AS.seek 0.82ml
@14:23:34.04 delay 3 seconds
@14:23:37.07 ARV.dial :waste
@14:23:37.71 delay 3 seconds
@14:23:40.74 ATV.dial :ARV
@14:23:41.66 AS.reconfigure ASconfig
@14:23:41.72 AS.seek 0.47ml
@14:24:02.13 AFV.select :carrierSolution
@14:24:02.25 delay 2 seconds
@14:24:04.27 AS.seek 1ml
@14:24:35.07 delay 1 second
@14:24:36.09 AFV.select :holdingCoil
@14:24:36.20 delay 1 second
@14:24:37.22 AS.reconfigure ASfast
@14:24:37.28 AS.seek 0ml
@14:25:04.63 AFV.select :carrierSolution
@14:25:04.74 delay 2 seconds
@14:25:06.76 AS.reconfigure ASconfig
@14:25:06.81 AS.seek 1ml
@14:26:04.57 delay 1 second
@14:26:05.59 AFV.select :holdingCoil
@14:26:05.70 delay 1 second
@14:26:06.72 AS.reconfigure ASfast
@14:26:06.77 AS.seek 0ml
@14:26:34.10 AFV.select :carrierSolution
@14:26:34.22 delay 2 seconds
@14:26:36.24 AS.reconfigure ASconfig
@14:26:36.29 AS.seek 1ml
@14:27:34.05 delay 1 second
@14:27:35.07 AFV.select :holdingCoil
@14:27:35.18 delay 1 second
@14:27:36.20 AS.reconfigure ASfast
@14:27:36.25 AS.seek 0ml
@14:28:03.60 ARV.dial between carrier and air1
@14:28:04.13 ATV.dial :air3
@14:28:05.50 AS.reconfigure ASslow1
@14:28:05.56 AS.seek 0.01ml
@14:28:07.91 delay 2 seconds
@14:28:09.94 ATV.dial :bleach
@14:28:10.58 AS.seek 0.81ml
@14:30:51.71 delay 3 seconds
@14:30:54.74 ATV.dial :air3
@14:30:55.32 delay 3 seconds
@14:30:58.39 AS.seek 0.835ml
@14:31:03.71 delay 3 seconds
@14:31:06.74 ATV.dial :bleach
@14:31:07.38 AS.seek 0.82ml
@14:31:10.71 delay 3 seconds
@14:31:13.74 ATV.dial PCR
@14:31:14.16 AS.reconfigure ASconfig
@14:31:14.21 AS.seek 0ml
@14:32:01.61 PCR.program :bleachProfile
@14:32:01.68 PCR program should complete in < 2:45
@14:32:01.69 PCR.run 10:00
@14:34:57.90 PCR.off
@14:34:57.94 AFV.select :carrierSolution
@14:34:58.05 delay 2 seconds
@14:35:00.07 AS.seek 1ml
@14:35:57.86 delay 1 second
@14:35:58.88 AFV.select :holdingCoil
@14:35:58.99 delay 1 second
@14:36:00.01 AS.reconfigure ASfast
@14:36:00.06 AS.seek 0ml
@14:36:27.41 delay 3 seconds
@14:36:30.43 AFV.select :carrierSolution
@14:36:30.54 delay 2 seconds
@14:36:32.56 AS.reconfigure ASconfig
@14:36:32.62 AS.seek 1ml
@14:37:30.36 delay 1 second
@14:37:31.38 AFV.select :holdingCoil
@14:37:31.49 delay 1 second
@14:37:32.51 AS.reconfigure ASfast
@14:37:32.56 AS.seek 0ml
@14:37:59.90 delay 3 seconds
@14:38:02.92 AFV.select :carrierSolution
@14:38:03.03 delay 2 seconds
@14:38:05.05 AS.reconfigure ASconfig
@14:38:05.10 AS.seek 1ml
@14:39:02.85 delay 1 second
@14:39:03.87 AFV.select :holdingCoil
@14:39:03.98 delay 1 second
@14:39:05.00 AS.reconfigure ASfast
@14:39:05.06 AS.seek 0ml
@14:39:32.38 delay 3 seconds
@14:39:35.40 AFV.select :carrierSolution
@14:39:35.51 delay 2 seconds
@14:39:37.53 AS.reconfigure ASconfig
@14:39:37.60 AS.seek 1ml
@14:40:35.35 delay 1 second
@14:40:36.37 AFV.select :holdingCoil
@14:40:36.48 delay 1 second
@14:40:37.50 AS.reconfigure ASfast
@14:40:37.55 AS.seek 0ml
@14:41:04.88 delay 3 seconds
@14:41:07.90 AFV.select :carrierSolution
@14:41:08.01 delay 2 seconds
@14:41:10.03 AS.reconfigure ASconfig
@14:41:10.08 AS.seek 1ml
@14:42:07.83 delay 1 second
@14:42:08.85 AFV.select :holdingCoil
@14:42:08.96 delay 1 second
@14:42:09.98 AS.reconfigure ASnorm
@14:42:10.03 AS.seek 0ml
@14:43:00.77 Setting up PCR for AOPP1 -- writing /var/log/esp/AOPP121mar2614h.pcr
@14:43:00.79 Building PCR Slug from ASV with MM1 mix, PP1 primer
@14:43:00.81 Priming with carrier
@14:43:00.82 ATV.dial ARV
@14:43:01.46 ARV.dial :carrier
@14:43:01.82 AS.seek 0.5ml
@14:43:27.36 AFV.select :carrierSolution
@14:43:27.48 delay 2 seconds
@14:43:29.50 AS.reconfigure ASconfig
@14:43:29.57 AS.seek 1ml
@14:43:58.60 delay 1 second
@14:43:59.62 AFV.select :holdingCoil
@14:43:59.73 delay 1 second
@14:44:00.75 ATV.dial :waste
@14:44:02.53 AS.reconfigure ASnorm
@14:44:02.59 AS.seek 0ml
@14:44:53.32 AFV.select :carrierSolution
@14:44:53.44 delay 2 seconds
@14:44:55.46 AS.reconfigure ASconfig
@14:44:55.52 AS.seek 0.5ml
@14:45:24.55 delay 1 second
@14:45:25.57 AFV.select :holdingCoil
@14:45:25.68 Building Slug
@14:45:25.69 ATV.dial :ARV
@14:45:27.44 delay 2 seconds
@14:45:29.47 ARV.dial :air1
@14:45:29.87 delay 2 seconds
@14:45:31.94 AS.reconfigure ASslow2
@14:45:32.00 AS.seek 0.506ml
@14:45:38.30 delay 2 seconds
@14:45:40.33 ARV.dial :MM1
@14:45:40.96 delay 2 seconds
@14:45:42.99 Adding MM1
@14:45:43.06 AS.seek 0.524ml
@14:46:01.33 delay 25 seconds
@14:46:26.36 ARV.dial :PP1
@14:46:26.76 Adding PP1
@14:46:26.77 delay 2 seconds
@14:46:28.84 AS.seek 0.53ml
@14:46:35.02 delay 10 seconds
@14:46:45.06 ARV.dial :air1
@14:46:45.48 adding ARV air
@14:46:45.49 delay 2 seconds
@14:46:47.56 AS.seek 0.536ml
@14:46:53.90 delay 5 seconds
@14:46:58.93 ARV.dial :carrier
@14:46:59.35 delay 7 seconds
@14:47:06.42 AS.seek 0.594ml
@14:48:05.00 delay 5 seconds
@14:48:10.03 ATV.dial :ASV
@14:48:10.44 delay 2 seconds
@14:48:12.51 AS.seek 0.6ml
@14:48:18.96 delay 4 seconds
@14:48:22.99 ATV.dial ARV
@14:48:23.57 delay 2 seconds
@14:48:25.60 ARV.dial :carrier
@14:48:25.61 delay 2 seconds
@14:48:27.68 AS.seek 0.65ml
@14:49:18.49 delay 4 seconds
@14:49:22.52 ATV.dial PCR
@14:49:23.16 ASV.dial :waste1
@14:49:23.18 ARV.dial :air1
@14:49:23.60 Positioning Slug by 0.324ml
@14:49:23.68 AS.seek 0.326ml
@14:54:49.90 delay 5 seconds
@14:54:55.36 ATV.dial ARV
@14:54:56.16 ASV.dial :PCRlock
@14:54:56.57 delay 5 seconds
@14:55:01.60 Running AOPP1 Profile60CIQ1 thermocycling
@14:55:01.61 <PCR> PCR.program Profile60CIQ1
@14:55:01.73 Profile60CIQ1 should complete in < 1:56:15
@14:55:01.74 PCR.run 2:30:18.75
@15:15:05.98 <mwj> -> exit
@15:15:05.99 Session Ended
@16:34:07.07 <PCR> PCR.off
@16:34:07.61 <MFBPCR> Running AOPP1 MeltProfile60 thermocycling
@16:34:07.63 <PCR> PCR.program MeltProfile60
@16:34:07.72 MeltProfile60 should complete in < 11:55
@16:34:07.73 PCR.run 19:53.75
@16:41:42.49 PCR.off
@16:41:43.40 <MFBPCR> PCR for AOPP1 completed -- closed /var/log/esp/AOPP121mar2614h.pcr
@16:41:43.45 Cleaning PCR
@16:41:43.46 AFV.select :carrierSolution
@16:41:43.57 delay 2 seconds
@16:41:45.59 AS.reconfigure ASconfig
@16:41:45.64 AS.seek 0.5ml
@16:41:55.91 delay 1 second
@16:41:56.93 AFV.select :holdingCoil
@16:41:57.04 ASV.dial :waste1
@16:41:57.43 ATV.dial :air3
@16:41:58.77 AS.reconfigure ASslow1
@16:41:58.81 AS.seek 0.51ml
@16:42:01.10 delay 2 seconds
@16:42:03.13 ATV.dial :bleach
@16:42:03.75 AS.seek 0.81ml
@16:43:04.32 delay 3 seconds
@16:43:07.35 ATV.dial :air3
@16:43:07.91 delay 3 seconds
@16:43:10.98 AS.seek 0.835ml
@16:43:16.29 delay 3 seconds
@16:43:19.32 ATV.dial :bleach
@16:43:19.94 AS.seek 0.82ml
@16:43:23.35 delay 3 seconds
@16:43:26.38 ARV.dial :waste
@16:43:26.99 delay 3 seconds
@16:43:30.02 ATV.dial :ARV
@16:43:30.93 AS.reconfigure ASconfig
@16:43:30.97 AS.seek 0.47ml
@16:43:51.35 AFV.select :carrierSolution
@16:43:51.46 delay 2 seconds
@16:43:53.48 AS.seek 1ml
@16:44:24.26 delay 1 second
@16:44:25.28 AFV.select :holdingCoil
@16:44:25.39 delay 1 second
@16:44:26.41 AS.reconfigure ASfast
@16:44:26.46 AS.seek 0ml
@16:44:53.79 AFV.select :carrierSolution
@16:44:53.90 delay 2 seconds
@16:44:55.92 AS.reconfigure ASconfig
@16:44:55.97 AS.seek 1ml
@16:45:53.71 delay 1 second
@16:45:54.73 AFV.select :holdingCoil
@16:45:54.84 delay 1 second
@16:45:55.86 AS.reconfigure ASfast
@16:45:55.91 AS.seek 0ml
@16:46:23.35 AFV.select :carrierSolution
@16:46:23.46 delay 2 seconds
@16:46:25.48 AS.reconfigure ASconfig
@16:46:25.53 AS.seek 1ml
@16:47:23.27 delay 1 second
@16:47:24.29 AFV.select :holdingCoil
@16:47:24.40 delay 1 second
@16:47:25.42 AS.reconfigure ASfast
@16:47:25.47 AS.seek 0ml
@16:47:52.79 ARV.dial between carrier and air1
@16:47:53.30 ATV.dial :air3
@16:47:54.66 AS.reconfigure ASslow1
@16:47:54.70 AS.seek 0.01ml
@16:47:57.13 delay 2 seconds
@16:47:59.16 ATV.dial :bleach
@16:47:59.78 AS.seek 0.81ml
@16:50:40.91 delay 3 seconds
@16:50:43.94 ATV.dial :air3
@16:50:44.51 delay 3 seconds
@16:50:47.58 AS.seek 0.835ml
@16:50:52.86 delay 3 seconds
@16:50:55.89 ATV.dial :bleach
@16:50:56.52 AS.seek 0.82ml
@16:50:59.80 delay 3 seconds
@16:51:02.83 ATV.dial PCR
@16:51:03.23 AS.reconfigure ASconfig
@16:51:03.27 AS.seek 0ml
@16:51:50.66 PCR.program :bleachProfile
@16:51:50.73 PCR program should complete in < 2:45
@16:51:50.74 PCR.run 10:00
@16:54:46.92 PCR.off
@16:54:46.94 AFV.select :carrierSolution
@16:54:47.05 delay 2 seconds
@16:54:49.07 AS.seek 1ml
@16:55:46.82 delay 1 second
@16:55:47.84 AFV.select :holdingCoil
@16:55:47.95 delay 1 second
@16:55:48.97 AS.reconfigure ASfast
@16:55:49.02 AS.seek 0ml
@16:56:16.32 delay 3 seconds
@16:56:19.34 AFV.select :carrierSolution
@16:56:19.45 delay 2 seconds
@16:56:21.47 AS.reconfigure ASconfig
@16:56:21.53 AS.seek 1ml
@16:57:19.27 delay 1 second
@16:57:20.29 AFV.select :holdingCoil
@16:57:20.40 delay 1 second
@16:57:21.42 AS.reconfigure ASfast
@16:57:21.47 AS.seek 0ml
@16:57:48.79 delay 3 seconds
@16:57:51.81 AFV.select :carrierSolution
@16:57:51.92 delay 2 seconds
@16:57:53.94 AS.reconfigure ASconfig
@16:57:53.99 AS.seek 1ml
@16:58:51.74 delay 1 second
@16:58:52.76 AFV.select :holdingCoil
@16:58:52.87 delay 1 second
@16:58:53.89 AS.reconfigure ASfast
@16:58:53.94 AS.seek 0ml
@16:59:21.24 delay 3 seconds
@16:59:24.26 AFV.select :carrierSolution
@16:59:24.37 delay 2 seconds
@16:59:26.39 AS.reconfigure ASconfig
@16:59:26.44 AS.seek 1ml
@17:00:24.21 delay 1 second
@17:00:25.23 AFV.select :holdingCoil
@17:00:25.34 delay 1 second
@17:00:26.36 AS.reconfigure ASfast
@17:00:26.41 AS.seek 0ml
@17:00:53.72 delay 3 seconds
@17:00:56.74 AFV.select :carrierSolution
@17:00:56.85 delay 2 seconds
@17:00:58.87 AS.reconfigure ASconfig
@17:00:58.92 AS.seek 1ml
@17:01:56.65 delay 1 second
@17:01:57.67 AFV.select :holdingCoil
@17:01:57.78 delay 1 second
@17:01:58.80 AS.reconfigure ASnorm
@17:01:58.85 AS.seek 0ml
@17:02:49.55 Setting up PCR for AARPP4 -- writing /var/log/esp/AARPP421mar2617h.pcr
@17:02:49.56 Building PCR Slug from ASV with MM2 mix, PP4 primer
@17:02:49.57 Priming with carrier
@17:02:49.58 ATV.dial ARV
@17:02:50.19 ARV.dial :carrier
@17:02:50.53 AS.seek 0.5ml
@17:03:16.08 AFV.select :carrierSolution
@17:03:16.19 delay 2 seconds
@17:03:18.21 AS.reconfigure ASconfig
@17:03:18.26 AS.seek 1ml
@17:03:47.26 delay 1 second
@17:03:48.28 AFV.select :holdingCoil
@17:03:48.39 delay 1 second
@17:03:49.41 ATV.dial :waste
@17:03:51.17 AS.reconfigure ASnorm
@17:03:51.21 AS.seek 0ml
@17:04:41.93 AFV.select :carrierSolution
@17:04:42.04 delay 2 seconds
@17:04:44.06 AS.reconfigure ASconfig
@17:04:44.11 AS.seek 0.5ml
@17:05:13.10 delay 1 second
@17:05:14.12 AFV.select :holdingCoil
@17:05:14.23 Building Slug
@17:05:14.24 ATV.dial :ARV
@17:05:15.97 delay 2 seconds
@17:05:18.00 ARV.dial :air1
@17:05:18.39 delay 2 seconds
@17:05:20.46 AS.reconfigure ASslow2
@17:05:20.50 AS.seek 0.506ml
@17:05:26.91 delay 2 seconds
@17:05:28.94 ARV.dial :MM2
@17:05:30.49 delay 2 seconds
@17:05:32.52 Adding MM2
@17:05:32.58 AS.seek 0.524ml
@17:05:51.24 delay 25 seconds
@17:06:16.27 ARV.dial :PP4
@17:06:16.69 Adding PP4
@17:06:16.70 delay 2 seconds
@17:06:18.76 AS.seek 0.53ml
@17:06:24.99 delay 10 seconds
@17:06:35.03 ARV.dial :air3
@17:06:35.42 adding ARV air
@17:06:35.43 delay 2 seconds
@17:06:37.49 AS.seek 0.536ml
@17:06:44.07 delay 5 seconds
@17:06:49.10 ARV.dial :carrier
@17:06:50.41 delay 7 seconds
@17:06:57.50 AS.seek 0.594ml
@17:07:56.07 delay 5 seconds
@17:08:01.10 ATV.dial :ASV
@17:08:01.51 delay 2 seconds
@17:08:03.59 AS.seek 0.6ml
@17:08:09.91 delay 4 seconds
@17:08:13.94 ATV.dial ARV
@17:08:14.51 delay 2 seconds
@17:08:16.54 ARV.dial :carrier
@17:08:16.55 delay 2 seconds
@17:08:18.61 AS.seek 0.65ml
@17:09:09.27 delay 4 seconds
@17:09:13.30 ATV.dial PCR
@17:09:13.93 ASV.dial :waste1
@17:09:13.95 ARV.dial :air1
@17:09:14.34 Positioning Slug by 0.324ml
@17:09:14.41 AS.seek 0.326ml
@17:14:40.51 delay 5 seconds
@17:14:45.54 ATV.dial ARV
@17:14:46.35 ASV.dial :PCRlock
@17:14:46.74 delay 5 seconds
@17:14:51.77 Running AARPP4 Profile58CIQ2 thermocycling
@17:14:51.78 <PCR> PCR.program Profile58CIQ2
@17:14:51.88 Profile58CIQ2 should complete in < 1:36:15
@17:14:51.89 PCR.run 2:05:18.75
@18:35:27.07 PCR.off
@18:35:27.61 <MFBPCR> Running AARPP4 MeltProfile58 thermocycling
@18:35:27.62 <PCR> PCR.program MeltProfile58
@18:35:27.71 MeltProfile58 should complete in < 12:33
@18:35:27.72 PCR.run 20:41.25
@18:43:07.07 PCR.off
@18:43:08.02 <MFBPCR> PCR for AARPP4 completed -- closed /var/log/esp/AARPP421mar2617h.pcr
@18:43:08.08 Cleaning PCR
@18:43:08.09 AFV.select :carrierSolution
@18:43:08.20 delay 2 seconds
@18:43:10.22 AS.reconfigure ASconfig
@18:43:10.27 AS.seek 0.5ml
@18:43:20.57 delay 1 second
@18:43:21.59 AFV.select :holdingCoil
@18:43:21.70 ASV.dial :waste1
@18:43:22.09 ATV.dial :air3
@18:43:23.44 AS.reconfigure ASslow1
@18:43:23.48 AS.seek 0.51ml
@18:43:25.77 delay 2 seconds
@18:43:27.79 ATV.dial :bleach
@18:43:28.41 AS.seek 0.81ml
@18:44:28.98 delay 3 seconds
@18:44:32.01 ATV.dial :air3
@18:44:32.57 delay 3 seconds
@18:44:35.64 AS.seek 0.835ml
@18:44:40.94 delay 3 seconds
@18:44:43.97 ATV.dial :bleach
@18:44:44.58 AS.seek 0.82ml
@18:44:47.88 delay 3 seconds
@18:44:50.91 ARV.dial :waste
@18:44:51.52 delay 3 seconds
@18:44:54.55 ATV.dial :ARV
@18:44:55.46 AS.reconfigure ASconfig
@18:44:55.50 AS.seek 0.47ml
@18:45:15.88 AFV.select :carrierSolution
@18:45:15.99 delay 2 seconds
@18:45:18.01 AS.seek 1ml
@18:45:48.76 delay 1 second
@18:45:49.78 AFV.select :holdingCoil
@18:45:49.89 delay 1 second
@18:45:50.91 AS.reconfigure ASfast
@18:45:50.96 AS.seek 0ml
@18:46:18.27 AFV.select :carrierSolution
@18:46:18.38 delay 2 seconds
@18:46:20.40 AS.reconfigure ASconfig
@18:46:20.45 AS.seek 1ml
@18:47:18.18 delay 1 second
@18:47:19.20 AFV.select :holdingCoil
@18:47:19.31 delay 1 second
@18:47:20.33 AS.reconfigure ASfast
@18:47:20.38 AS.seek 0ml
@18:47:47.68 AFV.select :carrierSolution
@18:47:47.79 delay 2 seconds
@18:47:49.81 AS.reconfigure ASconfig
@18:47:49.86 AS.seek 1ml
@18:48:47.60 delay 1 second
@18:48:48.62 AFV.select :holdingCoil
@18:48:48.73 delay 1 second
@18:48:49.75 AS.reconfigure ASfast
@18:48:49.80 AS.seek 0ml
@18:49:17.10 ARV.dial between carrier and air1
@18:49:17.62 ATV.dial :air3
@18:49:18.97 AS.reconfigure ASslow1
@18:49:19.01 AS.seek 0.01ml
@18:49:21.29 delay 2 seconds
@18:49:23.32 ATV.dial :bleach
@18:49:23.94 AS.seek 0.81ml
@18:52:05.07 delay 3 seconds
@18:52:08.10 ATV.dial :air3
@18:52:08.66 delay 3 seconds
@18:52:11.73 AS.seek 0.835ml
@18:52:17.00 delay 3 seconds
@18:52:20.03 ATV.dial :bleach
@18:52:20.66 AS.seek 0.82ml
@18:52:23.93 delay 3 seconds
@18:52:26.96 ATV.dial PCR
@18:52:27.78 AS.reconfigure ASconfig
@18:52:27.82 AS.seek 0ml
@18:53:15.23 PCR.program :bleachProfile
@18:53:15.30 PCR program should complete in < 2:45
@18:53:15.31 PCR.run 10:00
@18:56:11.08 PCR.off
@18:56:11.10 AFV.select :carrierSolution
@18:56:11.21 delay 2 seconds
@18:56:13.23 AS.seek 1ml
@18:57:10.96 delay 1 second
@18:57:11.98 AFV.select :holdingCoil
@18:57:12.09 delay 1 second
@18:57:13.11 AS.reconfigure ASfast
@18:57:13.16 AS.seek 0ml
@18:57:40.46 delay 3 seconds
@18:57:43.48 AFV.select :carrierSolution
@18:57:43.59 delay 2 seconds
@18:57:45.61 AS.reconfigure ASconfig
@18:57:45.66 AS.seek 1ml
@18:58:43.38 delay 1 second
@18:58:44.40 AFV.select :holdingCoil
@18:58:44.51 delay 1 second
@18:58:45.53 AS.reconfigure ASfast
@18:58:45.58 AS.seek 0ml
@18:59:12.90 delay 3 seconds
@18:59:15.92 AFV.select :carrierSolution
@18:59:16.03 delay 2 seconds
@18:59:18.05 AS.reconfigure ASconfig
@18:59:18.10 AS.seek 1ml
@19:00:15.84 delay 1 second
@19:00:16.86 AFV.select :holdingCoil
@19:00:16.97 delay 1 second
@19:00:17.99 AS.reconfigure ASfast
@19:00:18.04 AS.seek 0ml
@19:00:45.38 delay 3 seconds
@19:00:48.41 AFV.select :carrierSolution
@19:00:48.51 delay 2 seconds
@19:00:50.53 AS.reconfigure ASconfig
@19:00:50.59 AS.seek 1ml
@19:01:48.37 delay 1 second
@19:01:49.39 AFV.select :holdingCoil
@19:01:49.50 delay 1 second
@19:01:50.52 AS.reconfigure ASfast
@19:01:50.57 AS.seek 0ml
@19:02:17.91 delay 3 seconds
@19:02:20.93 AFV.select :carrierSolution
@19:02:21.03 delay 2 seconds
@19:02:23.05 AS.reconfigure ASconfig
@19:02:23.10 AS.seek 1ml
@19:03:20.87 delay 1 second
@19:03:21.89 AFV.select :holdingCoil
@19:03:22.00 delay 1 second
@19:03:23.02 AS.reconfigure ASnorm
@19:03:23.07 AS.seek 0ml
@19:04:13.79 Setting up PCR for AALPP3 -- writing /var/log/esp/AALPP321mar2619h.pcr
@19:04:13.80 Building PCR Slug from ASV with MM2 mix, PP3 primer
@19:04:13.81 Priming with carrier
@19:04:13.82 ATV.dial ARV
@19:04:14.45 ARV.dial :carrier
@19:04:14.80 AS.seek 0.5ml
@19:04:40.35 AFV.select :carrierSolution
@19:04:40.46 delay 2 seconds
@19:04:42.48 AS.reconfigure ASconfig
@19:04:42.53 AS.seek 1ml
@19:05:11.54 delay 1 second
@19:05:12.56 AFV.select :holdingCoil
@19:05:12.67 delay 1 second
@19:05:13.69 ATV.dial :waste
@19:05:15.46 AS.reconfigure ASnorm
@19:05:15.90 AS.seek 0ml
@19:06:06.60 AFV.select :carrierSolution
@19:06:06.71 delay 2 seconds
@19:06:08.73 AS.reconfigure ASconfig
@19:06:08.78 AS.seek 0.5ml
@19:06:37.80 delay 1 second
@19:06:38.82 AFV.select :holdingCoil
@19:06:38.93 Building Slug
@19:06:38.94 ATV.dial :ARV
@19:06:40.66 delay 2 seconds
@19:06:42.69 ARV.dial :air1
@19:06:43.07 delay 2 seconds
@19:06:45.14 AS.reconfigure ASslow2
@19:06:45.19 AS.seek 0.506ml
@19:06:51.52 delay 2 seconds
@19:06:53.55 ARV.dial :MM2
@19:06:55.10 delay 2 seconds
@19:06:57.13 Adding MM2
@19:06:57.19 AS.seek 0.524ml
@19:07:15.60 delay 25 seconds
@19:07:40.63 ARV.dial :PP3
@19:07:41.02 Adding PP3
@19:07:41.03 delay 2 seconds
@19:07:43.09 AS.seek 0.53ml
@19:07:49.38 delay 10 seconds
@19:07:59.42 ARV.dial :air2
@19:07:59.82 adding ARV air
@19:07:59.84 delay 2 seconds
@19:08:01.90 AS.seek 0.536ml
@19:08:08.45 delay 5 seconds
@19:08:13.48 ARV.dial :carrier
@19:08:14.80 delay 7 seconds
@19:08:21.87 AS.seek 0.594ml
@19:09:20.63 delay 5 seconds
@19:09:25.66 ATV.dial :ASV
@19:09:26.07 delay 2 seconds
@19:09:28.14 AS.seek 0.6ml
@19:09:34.65 delay 4 seconds
@19:09:38.68 ATV.dial ARV
@19:09:39.24 delay 2 seconds
@19:09:41.27 ARV.dial :carrier
@19:09:41.28 delay 2 seconds
@19:09:43.34 AS.seek 0.65ml
@19:10:34.09 delay 4 seconds
@19:10:38.12 ATV.dial PCR
@19:10:38.76 ASV.dial :waste1
@19:10:38.78 ARV.dial :air1
@19:10:39.17 Positioning Slug by 0.324ml
@19:10:39.24 AS.seek 0.326ml
@19:16:05.20 delay 5 seconds
@19:16:10.23 ATV.dial ARV
@19:16:11.02 ASV.dial :PCRlock
@19:16:11.41 delay 5 seconds
@19:16:16.44 Running AALPP3 Profile58CIQ2 thermocycling
@19:16:16.45 <PCR> PCR.program Profile58CIQ2
@19:16:16.55 Profile58CIQ2 should complete in < 1:36:15
@19:16:16.56 PCR.run 2:05:18.75
@20:37:01.10 PCR.off
@20:37:01.65 <MFBPCR> Running AALPP3 MeltProfile58 thermocycling
@20:37:01.66 <PCR> PCR.program MeltProfile58
@20:37:01.75 MeltProfile58 should complete in < 12:33
@20:37:01.76 PCR.run 20:41.25
@20:44:46.04 PCR.off
@20:44:47.00 <MFBPCR> PCR for AALPP3 completed -- closed /var/log/esp/AALPP321mar2619h.pcr
@20:44:47.06 Cleaning PCR
@20:44:47.07 AFV.select :carrierSolution
@20:44:47.17 delay 2 seconds
@20:44:49.19 AS.reconfigure ASconfig
@20:44:49.24 AS.seek 0.5ml
@20:44:59.51 delay 1 second
@20:45:00.53 AFV.select :holdingCoil
@20:45:00.64 ASV.dial :waste1
@20:45:01.03 ATV.dial :air3
@20:45:02.38 AS.reconfigure ASslow1
@20:45:02.42 AS.seek 0.51ml
@20:45:04.71 delay 2 seconds
@20:45:06.73 ATV.dial :bleach
@20:45:07.37 AS.seek 0.81ml
@20:46:08.01 delay 3 seconds
@20:46:11.04 ATV.dial :air3
@20:46:11.62 delay 3 seconds
@20:46:14.69 AS.seek 0.835ml
@20:46:20.07 delay 3 seconds
@20:46:23.10 ATV.dial :bleach
@20:46:23.73 AS.seek 0.82ml
@20:46:27.03 delay 3 seconds
@20:46:30.06 ARV.dial :waste
@20:46:30.67 delay 3 seconds
@20:46:33.70 ATV.dial :ARV
@20:46:34.62 AS.reconfigure ASconfig
@20:46:34.66 AS.seek 0.47ml
@20:46:55.06 AFV.select :carrierSolution
@20:46:55.17 delay 2 seconds
@20:46:57.19 AS.seek 1ml
@20:47:27.92 delay 1 second
@20:47:28.94 AFV.select :holdingCoil
@20:47:29.05 delay 1 second
@20:47:30.07 AS.reconfigure ASfast
@20:47:30.12 AS.seek 0ml
@20:47:57.84 AFV.select :carrierSolution
@20:47:57.95 delay 2 seconds
@20:47:59.97 AS.reconfigure ASconfig
@20:48:00.02 AS.seek 1ml
@20:48:57.76 delay 1 second
@20:48:58.78 AFV.select :holdingCoil
@20:48:58.89 delay 1 second
@20:48:59.91 AS.reconfigure ASfast
@20:48:59.96 AS.seek 0ml
@20:49:27.29 AFV.select :carrierSolution
@20:49:27.40 delay 2 seconds
@20:49:29.42 AS.reconfigure ASconfig
@20:49:29.47 AS.seek 1ml
@20:50:27.21 delay 1 second
@20:50:28.23 AFV.select :holdingCoil
@20:50:28.34 delay 1 second
@20:50:29.36 AS.reconfigure ASfast
@20:50:29.41 AS.seek 0ml
@20:50:56.71 ARV.dial between carrier and air1
@20:50:57.22 ATV.dial :air3
@20:50:58.57 AS.reconfigure ASslow1
@20:50:58.61 AS.seek 0.01ml
@20:51:00.99 delay 2 seconds
@20:51:03.02 ATV.dial :bleach
@20:51:03.65 AS.seek 0.81ml
@20:53:44.79 delay 3 seconds
@20:53:47.82 ATV.dial :air3
@20:53:48.38 delay 3 seconds
@20:53:51.45 AS.seek 0.835ml
@20:53:56.75 delay 3 seconds
@20:53:59.78 ATV.dial :bleach
@20:54:00.41 AS.seek 0.82ml
@20:54:03.67 delay 3 seconds
@20:54:06.70 ATV.dial PCR
@20:54:07.10 AS.reconfigure ASconfig
@20:54:07.14 AS.seek 0ml
@20:54:54.56 PCR.program :bleachProfile
@20:54:54.63 PCR program should complete in < 2:45
@20:54:54.64 PCR.run 10:00
@20:57:50.41 PCR.off
@20:57:50.43 AFV.select :carrierSolution
@20:57:50.54 delay 2 seconds
@20:57:52.56 AS.seek 1ml
@20:58:50.31 delay 1 second
@20:58:51.33 AFV.select :holdingCoil
@20:58:51.44 delay 1 second
@20:58:52.46 AS.reconfigure ASfast
@20:58:52.51 AS.seek 0ml
@20:59:19.82 delay 3 seconds
@20:59:22.84 AFV.select :carrierSolution
@20:59:22.95 delay 2 seconds
@20:59:24.97 AS.reconfigure ASconfig
@20:59:25.03 AS.seek 1ml
@21:00:22.76 delay 1 second
@21:00:23.78 AFV.select :holdingCoil
@21:00:23.89 delay 1 second
@21:00:24.91 AS.reconfigure ASfast
@21:00:24.96 AS.seek 0ml
@21:00:52.28 delay 3 seconds
@21:00:55.30 AFV.select :carrierSolution
@21:00:55.41 delay 2 seconds
@21:00:57.43 AS.reconfigure ASconfig
@21:00:57.48 AS.seek 1ml
@21:01:55.26 delay 1 second
@21:01:56.28 AFV.select :holdingCoil
@21:01:56.79 delay 1 second
@21:01:57.81 AS.reconfigure ASfast
@21:01:57.86 AS.seek 0ml
@21:02:25.20 delay 3 seconds
@21:02:28.22 AFV.select :carrierSolution
@21:02:28.33 delay 2 seconds
@21:02:30.35 AS.reconfigure ASconfig
@21:02:30.41 AS.seek 1ml
@21:03:28.13 delay 1 second
@21:03:29.15 AFV.select :holdingCoil
@21:03:29.26 delay 1 second
@21:03:30.28 AS.reconfigure ASfast
@21:03:30.33 AS.seek 0ml
@21:03:57.65 delay 3 seconds
@21:04:00.67 AFV.select :carrierSolution
@21:04:00.78 delay 2 seconds
@21:04:02.80 AS.reconfigure ASconfig
@21:04:02.85 AS.seek 1ml
@21:05:00.62 delay 1 second
@21:05:01.64 AFV.select :holdingCoil
@21:05:01.75 delay 1 second
@21:05:02.77 AS.reconfigure ASnorm
@21:05:02.82 AS.seek 0ml
@21:05:53.54 Setting up PCR for ACOPP5 -- writing /var/log/esp/ACOPP521mar2621h.pcr
@21:05:53.55 Building PCR Slug from ASV with MM3 mix, PP5 primer
@21:05:53.56 Priming with carrier
@21:05:53.57 ATV.dial ARV
@21:05:54.20 ARV.dial :carrier
@21:05:54.54 AS.seek 0.5ml
@21:06:20.06 AFV.select :carrierSolution
@21:06:20.17 delay 2 seconds
@21:06:22.19 AS.reconfigure ASconfig
@21:06:22.24 AS.seek 1ml
@21:06:51.23 delay 1 second
@21:06:52.25 AFV.select :holdingCoil
@21:06:52.36 delay 1 second
@21:06:53.38 ATV.dial :waste
@21:06:55.14 AS.reconfigure ASnorm
@21:06:55.18 AS.seek 0ml
@21:07:45.87 AFV.select :carrierSolution
@21:07:45.98 delay 2 seconds
@21:07:48.00 AS.reconfigure ASconfig
@21:07:48.05 AS.seek 0.5ml
@21:08:17.10 delay 1 second
@21:08:18.12 AFV.select :holdingCoil
@21:08:18.23 Building Slug
@21:08:18.24 ATV.dial :ARV
@21:08:19.98 delay 2 seconds
@21:08:22.01 ARV.dial :air1
@21:08:22.39 delay 2 seconds
@21:08:24.46 AS.reconfigure ASslow2
@21:08:24.51 AS.seek 0.506ml
@21:08:30.74 delay 2 seconds
@21:08:32.77 ARV.dial :MM3
@21:08:33.85 delay 2 seconds
@21:08:35.88 Adding MM3
@21:08:35.95 AS.seek 0.524ml
@21:08:54.32 delay 25 seconds
@21:09:19.35 ARV.dial :PP5
@21:09:19.73 Adding PP5
@21:09:19.75 delay 2 seconds
@21:09:21.81 AS.seek 0.53ml
@21:09:28.48 delay 10 seconds
@21:09:38.52 ARV.dial :air3
@21:09:38.90 adding ARV air
@21:09:38.92 delay 2 seconds
@21:09:40.98 AS.seek 0.536ml
@21:09:47.29 delay 5 seconds
@21:09:52.32 ARV.dial :carrier
@21:09:53.63 delay 7 seconds
@21:10:00.70 AS.seek 0.594ml
@21:10:59.32 delay 5 seconds
@21:11:04.35 ATV.dial :ASV
@21:11:04.74 delay 2 seconds
@21:11:06.81 AS.seek 0.6ml
@21:11:13.06 delay 4 seconds
@21:11:17.09 ATV.dial ARV
@21:11:17.65 delay 2 seconds
@21:11:19.68 ARV.dial :carrier
@21:11:19.69 delay 2 seconds
@21:11:21.75 AS.seek 0.65ml
@21:12:12.43 delay 4 seconds
@21:12:16.46 ATV.dial PCR
@21:12:17.09 ASV.dial :waste1
@21:12:17.11 ARV.dial :air1
@21:12:17.50 Positioning Slug by 0.324ml
@21:12:17.57 AS.seek 0.326ml
@21:17:43.48 delay 5 seconds
@21:17:48.51 ATV.dial ARV
@21:17:49.31 ASV.dial :PCRlock
@21:17:49.70 delay 5 seconds
@21:17:54.73 Running ACOPP5 ProfileIPc thermocycling
@21:17:54.74 <PCR> PCR.program ProfileIPc
@21:17:54.84 ProfileIPc should complete in < 1:26:35
@21:17:54.85 PCR.run 1:53:13.75
@22:26:35.90 PCR.off
@22:26:36.45 <MFBPCR> PCR for ACOPP5 completed -- closed /var/log/esp/ACOPP521mar2621h.pcr
@22:26:36.50 Cleaning PCR
@22:26:36.51 AFV.select :carrierSolution
@22:26:36.62 delay 2 seconds
@22:26:38.64 AS.reconfigure ASconfig
@22:26:38.69 AS.seek 0.5ml
@22:26:48.98 delay 1 second
@22:26:50.00 AFV.select :holdingCoil
@22:26:50.10 ASV.dial :waste1
@22:26:50.48 ATV.dial :air3
@22:26:51.84 AS.reconfigure ASslow1
@22:26:51.88 AS.seek 0.51ml
@22:26:54.21 delay 2 seconds
@22:26:56.24 ATV.dial :bleach
@22:26:56.87 AS.seek 0.81ml
@22:27:57.50 delay 3 seconds
@22:28:00.53 ATV.dial :air3
@22:28:01.09 delay 3 seconds
@22:28:04.16 AS.seek 0.835ml
@22:28:09.44 delay 3 seconds
@22:28:12.47 ATV.dial :bleach
@22:28:13.10 AS.seek 0.82ml
@22:28:16.40 delay 3 seconds
@22:28:19.43 ARV.dial :waste
@22:28:20.04 delay 3 seconds
@22:28:23.07 ATV.dial :ARV
@22:28:24.37 AS.reconfigure ASconfig
@22:28:24.41 AS.seek 0.47ml
@22:28:44.78 AFV.select :carrierSolution
@22:28:44.89 delay 2 seconds
@22:28:46.91 AS.seek 1ml
@22:29:17.68 delay 1 second
@22:29:18.70 AFV.select :holdingCoil
@22:29:18.81 delay 1 second
@22:29:19.83 AS.reconfigure ASfast
@22:29:19.88 AS.seek 0ml
@22:29:47.20 AFV.select :carrierSolution
@22:29:47.31 delay 2 seconds
@22:29:49.33 AS.reconfigure ASconfig
@22:29:49.38 AS.seek 1ml
@22:30:47.10 delay 1 second
@22:30:48.12 AFV.select :holdingCoil
@22:30:48.23 delay 1 second
@22:30:49.25 AS.reconfigure ASfast
@22:30:49.30 AS.seek 0ml
@22:31:16.62 AFV.select :carrierSolution
@22:31:16.73 delay 2 seconds
@22:31:18.75 AS.reconfigure ASconfig
@22:31:18.80 AS.seek 1ml
@22:32:16.54 delay 1 second
@22:32:17.56 AFV.select :holdingCoil
@22:32:17.67 delay 1 second
@22:32:18.69 AS.reconfigure ASfast
@22:32:18.74 AS.seek 0ml
@22:32:46.07 ARV.dial between carrier and air1
@22:32:46.59 ATV.dial :air3
@22:32:47.95 AS.reconfigure ASslow1
@22:32:47.99 AS.seek 0.01ml
@22:32:50.31 delay 2 seconds
@22:32:52.34 ATV.dial :bleach
@22:32:52.96 AS.seek 0.81ml
@22:35:34.15 delay 3 seconds
@22:35:37.18 ATV.dial :air3
@22:35:37.75 delay 3 seconds
@22:35:40.82 AS.seek 0.835ml
@22:35:46.12 delay 3 seconds
@22:35:49.15 ATV.dial :bleach
@22:35:49.77 AS.seek 0.82ml
@22:35:53.12 delay 3 seconds
@22:35:56.15 ATV.dial PCR
@22:35:56.55 AS.reconfigure ASconfig
@22:35:56.59 AS.seek 0ml
@22:36:44.01 PCR.program :bleachProfile
@22:36:44.07 PCR program should complete in < 2:45
@22:36:44.08 PCR.run 10:00
@22:39:39.86 PCR.off
@22:39:39.88 AFV.select :carrierSolution
@22:39:39.99 delay 2 seconds
@22:39:42.01 AS.seek 1ml
@22:40:39.81 delay 1 second
@22:40:40.83 AFV.select :holdingCoil
@22:40:40.94 delay 1 second
@22:40:41.96 AS.reconfigure ASfast
@22:40:42.01 AS.seek 0ml
@22:41:09.32 delay 3 seconds
@22:41:12.34 AFV.select :carrierSolution
@22:41:12.45 delay 2 seconds
@22:41:14.47 AS.reconfigure ASconfig
@22:41:14.53 AS.seek 1ml
@22:42:12.28 delay 1 second
@22:42:13.64 AFV.select :holdingCoil
@22:42:13.80 delay 1 second
@22:42:14.82 AS.reconfigure ASfast
@22:42:14.87 AS.seek 0ml
@22:42:42.20 delay 3 seconds
@22:42:45.22 AFV.select :carrierSolution
@22:42:45.33 delay 2 seconds
@22:42:47.35 AS.reconfigure ASconfig
@22:42:47.40 AS.seek 1ml
@22:43:45.12 delay 1 second
@22:43:46.14 AFV.select :holdingCoil
@22:43:46.25 delay 1 second
@22:43:47.27 AS.reconfigure ASfast
@22:43:47.32 AS.seek 0ml
@22:44:14.64 delay 3 seconds
@22:44:17.66 AFV.select :carrierSolution
@22:44:17.77 delay 2 seconds
@22:44:19.79 AS.reconfigure ASconfig
@22:44:19.84 AS.seek 1ml
@22:45:17.59 delay 1 second
@22:45:18.61 AFV.select :holdingCoil
@22:45:18.72 delay 1 second
@22:45:19.74 AS.reconfigure ASfast
@22:45:19.79 AS.seek 0ml
@22:45:47.12 delay 3 seconds
@22:45:50.14 AFV.select :carrierSolution
@22:45:50.25 delay 2 seconds
@22:45:52.27 AS.reconfigure ASconfig
@22:45:52.32 AS.seek 1ml
@22:46:50.06 delay 1 second
@22:46:51.08 AFV.select :holdingCoil
@22:46:51.19 delay 1 second
@22:46:52.21 AS.reconfigure ASnorm
@22:46:52.26 AS.seek 0ml
@22:47:42.99 Setting up PCR for AIPc -- writing /var/log/esp/AIPc21mar2622h.pcr
@22:47:43.00 Building PCR Slug from ASV with MM3 mix, PP6 primer
@22:47:43.01 Priming with carrier
@22:47:43.02 ATV.dial ARV
@22:47:43.65 ARV.dial :carrier
@22:47:43.99 AS.seek 0.5ml
@22:48:09.54 AFV.select :carrierSolution
@22:48:09.65 delay 2 seconds
@22:48:11.67 AS.reconfigure ASconfig
@22:48:11.72 AS.seek 1ml
@22:48:40.71 delay 1 second
@22:48:41.73 AFV.select :holdingCoil
@22:48:41.84 delay 1 second
@22:48:42.86 ATV.dial :waste
@22:48:44.65 AS.reconfigure ASnorm
@22:48:44.69 AS.seek 0ml
@22:49:35.40 AFV.select :carrierSolution
@22:49:35.51 delay 2 seconds
@22:49:37.53 AS.reconfigure ASconfig
@22:49:37.58 AS.seek 0.5ml
@22:50:06.60 delay 1 second
@22:50:07.62 AFV.select :holdingCoil
@22:50:07.73 Building Slug
@22:50:07.74 ATV.dial :ARV
@22:50:09.46 delay 2 seconds
@22:50:11.49 ARV.dial :air1
@22:50:11.88 delay 2 seconds
@22:50:13.95 AS.reconfigure ASslow2
@22:50:14.00 AS.seek 0.506ml
@22:50:20.23 delay 2 seconds
@22:50:22.26 ARV.dial :MM3
@22:50:23.32 delay 2 seconds
@22:50:25.35 Adding MM3
@22:50:25.41 AS.seek 0.524ml
@22:50:43.76 delay 25 seconds
@22:51:08.80 ARV.dial :PP6
@22:51:09.18 Adding PP6
@22:51:09.20 delay 2 seconds
@22:51:11.26 AS.seek 0.53ml
@22:51:17.68 delay 10 seconds
@22:51:27.72 ARV.dial :air1
@22:51:28.57 adding ARV air
@22:51:28.58 delay 2 seconds
@22:51:30.64 AS.seek 0.536ml
@22:51:37.17 delay 5 seconds
@22:51:42.20 ARV.dial :carrier
@22:51:42.59 delay 7 seconds
@22:51:49.66 AS.seek 0.594ml
@22:52:48.56 delay 5 seconds
@22:52:53.59 ATV.dial :ASV
@22:52:54.00 delay 2 seconds
@22:52:56.07 AS.seek 0.6ml
@22:53:02.32 delay 4 seconds
@22:53:06.35 ATV.dial ARV
@22:53:06.92 delay 2 seconds
@22:53:08.95 ARV.dial :carrier
@22:53:08.96 delay 2 seconds
@22:53:11.02 AS.seek 0.65ml
@22:54:01.84 delay 4 seconds
@22:54:05.87 ATV.dial PCR
@22:54:06.50 ASV.dial :waste1
@22:54:06.52 ARV.dial :air1
@22:54:06.92 Positioning Slug by 0.324ml
@22:54:06.98 AS.seek 0.326ml
@22:59:33.00 delay 5 seconds
@22:59:38.03 ATV.dial ARV
@22:59:38.82 ASV.dial :PCRlock
@22:59:39.21 delay 5 seconds
@22:59:44.24 Running AIPc ProfileIPc thermocycling
@22:59:44.25 <PCR> PCR.program ProfileIPc
@22:59:44.35 ProfileIPc should complete in < 1:26:35
@22:59:44.36 PCR.run 1:53:13.75
@00:08:24.58CET27-Mar-21 PCR.off
@00:08:25.13 <MFBPCR> PCR for AIPc completed -- closed /var/log/esp/AIPc21mar2622h.pcr
@00:08:25.14 Full MFB Decontamination
@00:08:25.15 Decon extraction column and decon extSrc
@00:08:25.17 SPE.hold 2:46:40,55C
@00:08:25.20 ASV.dial :SPEdelivery
@00:08:25.87 delay 20 seconds
@00:08:45.90 AFV.select :carrierSolution
@00:08:46.01 delay 2 seconds
@00:08:48.03 AS.reconfigure ASconfig
@00:08:48.08 AS.seek 0.5ml
@00:08:58.40 delay 1 second
@00:08:59.42 AFV.select :holdingCoil
@00:08:59.53 ATV.dial :air1
@00:09:01.80 AS.reconfigure ASslow1
@00:09:01.84 AS.seek 0.52ml
@00:09:06.04 ATV.dial :ASV
@00:09:07.81 ASV.dial :SPErecovery
@00:09:08.49 AS.seek 0.62ml
@00:09:28.89 ATV.dial :waste
@00:09:31.49 AS.reconfigure ASnorm
@00:09:31.53 AS.seek 0ml
@00:10:03.11 deconEluateRecoveryLine extSrc
@00:10:03.12 ATV.dial :waste
@00:10:03.14 AS.seek 0ml
@00:10:03.28 ATV.dial :air3
@00:10:04.03 AS.reconfigure ASslow1
@00:10:04.08 AS.seek 0.01ml
@00:10:06.34 delay 2 seconds
@00:10:08.37 ATV.dial :bleach
@00:10:08.87 AS.seek 0.31ml
@00:11:09.45 delay 3 seconds
@00:11:12.48 ATV.dial :air3
@00:11:13.11 delay 3 seconds
@00:11:16.18 AS.seek 0.335ml
@00:11:21.40 delay 3 seconds
@00:11:24.43 ATV.dial :bleach
@00:11:25.10 AS.seek 0.32ml
@00:11:28.47 delay 3 seconds
@00:11:31.50 ATV.dial :extSrc
@00:11:32.16 AS.seek 0ml
@00:12:36.89 AFV.select :carrierSolution
@00:12:37.00 delay 2 seconds
@00:12:39.02 AS.reconfigure ASconfig
@00:12:39.07 AS.seek 0.5ml
@00:13:08.07 delay 1 second
@00:13:09.09 AFV.select :holdingCoil
@00:13:09.20 ATV.dial :extSrc
@00:13:09.21 AS.reconfigure ASslow1
@00:13:09.26 AS.seek 0ml
@00:14:50.09 AFV.select :carrierSolution
@00:14:50.20 delay 2 seconds
@00:14:52.22 AS.reconfigure ASconfig
@00:14:52.27 AS.seek 0.5ml
@00:15:21.29 delay 1 second
@00:15:22.31 AFV.select :holdingCoil
@00:15:22.42 ATV.dial :extSrc
@00:15:22.43 AS.reconfigure ASslow1
@00:15:22.48 AS.seek 0ml
@00:17:03.28 AFV.select :carrierSolution
@00:17:03.39 delay 2 seconds
@00:17:05.41 AS.reconfigure ASconfig
@00:17:05.46 AS.seek 0.5ml
@00:17:34.48 delay 1 second
@00:17:35.50 AFV.select :holdingCoil
@00:17:35.61 ATV.dial :extSrc
@00:17:35.63 AS.reconfigure ASslow1
@00:17:35.67 AS.seek 0ml
@00:19:16.48 AFV.select :carrierSolution
@00:19:16.59 delay 2 seconds
@00:19:18.61 AS.reconfigure ASconfig
@00:19:18.66 AS.seek 0.5ml
@00:19:47.68 delay 1 second
@00:19:48.70 AFV.select :holdingCoil
@00:19:48.81 ATV.dial :extSrc
@00:19:48.82 AS.reconfigure ASslow1
@00:19:48.87 AS.seek 0ml
@00:21:29.67 ATV.dial :air2
@00:21:31.06 AS.reconfigure ASslow0
@00:21:31.10 AS.seek 0.5ml
@00:22:21.64 ATV.dial :extSrc
@00:22:23.20 AS.seek 0.02ml
@00:23:11.79 delay 1 second
@00:23:12.82 ATV.dial :waste
@00:23:14.18 AS.reconfigure ASconfig
@00:23:14.22 AS.seek 0ml
@00:23:15.64 AFV.select :holdingCoil
@00:23:15.65 ATV.dial :waste
@00:23:15.67 AS.seek 0ml
@00:23:15.73 AFV.select :carrierSolution
@00:23:15.83 delay 2 seconds
@00:23:17.85 AS.seek 0.5ml
@00:23:46.86 delay 1 second
@00:23:47.88 AFV.select :holdingCoil
@00:23:47.99 ATV.dial :air3
@00:23:48.85 AS.reconfigure ASslow1
@00:23:48.89 AS.seek 0.52ml
@00:23:53.22 ATV.dial :air3
@00:23:53.29 AS.seek 0.53ml
@00:23:55.58 delay 2 seconds
@00:23:57.61 ATV.dial :bleach
@00:23:58.06 AS.seek 0.71ml
@00:24:34.51 delay 3 seconds
@00:24:37.54 ATV.dial :air3
@00:24:38.11 delay 3 seconds
@00:24:41.18 AS.seek 0.735ml
@00:24:46.49 delay 3 seconds
@00:24:49.52 ATV.dial :bleach
@00:24:50.15 AS.seek 0.72ml
@00:24:53.47 delay 3 seconds
@00:24:56.50 ATV.dial ASV
@00:24:57.58 ASV.dial :SPErecovery
@00:24:57.65 AS.seek 0.365ml
@00:26:09.33 ATV.dial :waste
@00:26:11.35 AS.reconfigure ASnorm
@00:26:11.39 AS.seek 0ml
@00:26:30.12 ATV.dial :air3
@00:26:30.99 AS.reconfigure ASslow1
@00:26:31.03 AS.seek 0.01ml
@00:26:33.33 delay 2 seconds
@00:26:35.36 ATV.dial :bleach
@00:26:35.84 AS.seek 0.58ml
@00:28:30.72 delay 3 seconds
@00:28:33.75 ATV.dial :air3
@00:28:34.33 delay 3 seconds
@00:28:37.40 AS.seek 0.605ml
@00:28:42.71 delay 3 seconds
@00:28:45.74 ATV.dial :bleach
@00:28:46.80 AS.seek 0.59ml
@00:28:50.12 delay 3 seconds
@00:28:53.15 ATV.dial :ASV
@00:28:54.25 ASV.dial :wasteR
@00:28:54.65 AS.seek 0ml
@00:30:53.54 delay 1:00
@00:31:53.58 ATV.dial ASV
@00:31:53.59 AFV.select :carrierSolution
@00:31:53.69 delay 2 seconds
@00:31:55.71 AS.reconfigure ASconfig
@00:31:55.76 AS.seek 1ml
@00:32:53.51 delay 1 second
@00:32:54.53 AFV.select :holdingCoil
@00:32:54.64 delay 1 second
@00:32:55.66 ASV.dial :wasteR
@00:32:55.67 AS.reconfigure ASslow1
@00:32:55.72 AS.seek 0ml
@00:36:17.09 delay 10 seconds
@00:36:27.13 ATV.dial :air3
@00:36:28.73 AS.seek 0.01ml
@00:36:31.09 delay 2 seconds
@00:36:33.12 ATV.dial :bleach
@00:36:33.74 AS.seek 0.58ml
@00:38:28.68 delay 3 seconds
@00:38:31.71 ATV.dial :air3
@00:38:32.29 delay 3 seconds
@00:38:35.36 AS.seek 0.605ml
@00:38:40.66 delay 3 seconds
@00:38:43.69 ATV.dial :bleach
@00:38:44.34 AS.seek 0.59ml
@00:38:47.64 delay 3 seconds
@00:38:50.67 ATV.dial ASV
@00:38:51.75 ASV.dial :SPEdelivery
@00:38:52.13 AS.seek 0ml
@00:40:51.03 delay 1:00
@00:41:51.06 AFV.select :carrierSolution
@00:41:51.17 delay 2 seconds
@00:41:53.19 AS.reconfigure ASconfig
@00:41:53.24 AS.seek 1ml
@00:42:50.98 delay 1 second
@00:42:52.00 AFV.select :holdingCoil
@00:42:52.11 delay 1 second
@00:42:53.13 ATV.dial ASV
@00:42:53.14 ASV.dial :SPEdelivery
@00:42:53.15 AS.reconfigure ASslow1
@00:42:53.20 AS.seek 0ml
@00:46:14.67 delay 10 seconds
@00:46:24.71 ATV.dial :air2
@00:46:25.59 AS.reconfigure ASslow0
@00:46:25.63 AS.seek 0.8ml
@00:47:46.39 delay 3 seconds
@00:47:49.42 ATV.dial :ASV
@00:47:50.47 ASV.dial :SPEdelivery
@00:47:50.49 AS.reconfigure ASslow1
@00:47:50.53 AS.seek 0.03ml
@00:50:25.64 delay 20 seconds
@00:50:45.68 ATV.dial :waste,via: :air3
@00:50:47.54 AS.reconfigure ASconfig
@00:50:47.58 AS.seek 0ml
@00:50:49.61 SPE.coast
@00:50:49.70 Removing Bleach from Mixing Coil
@00:50:49.71 AFV.select :carrierSolution
@00:50:49.82 delay 2 seconds
@00:50:51.84 AS.seek 0.5ml
@00:51:20.84 delay 1 second
@00:51:21.86 AFV.select :holdingCoil
@00:51:21.97 ATV.dial :ASV
@00:51:23.78 ASV.dial :SPErecovery
@00:51:24.43 AS.reconfigure ASslow1
@00:51:24.47 AS.seek 0.73ml
@00:52:11.00 ASV.dial :wasteD
@00:52:11.60 AS.reconfigure ASnorm
@00:52:11.64 AS.seek 0ml
@00:52:48.73 AFV.select :carrierSolution
@00:52:48.84 delay 2 seconds
@00:52:50.86 AS.reconfigure ASconfig
@00:52:50.91 AS.seek 0.5ml
@00:53:19.97 delay 1 second
@00:53:20.99 AFV.select :holdingCoil
@00:53:21.10 ATV.dial ASV
@00:53:21.11 ASV.dial :SPErecovery
@00:53:21.76 AS.reconfigure ASslow0
@00:53:21.80 AS.seek 0.3ml
@00:53:42.20 ATV.dial :air2
@00:53:43.31 AS.reconfigure ASslow1
@00:53:43.35 AS.seek 0.32ml
@00:53:47.73 ATV.dial ASV
@00:53:48.84 AS.reconfigure ASslow0
@00:53:48.88 AS.seek 0.57ml
@00:54:14.33 ASV.dial :wasteD
@00:54:14.93 AS.reconfigure ASnorm
@00:54:14.97 AS.seek 0ml
@00:54:44.03 AFV.select :carrierSolution
@00:54:44.14 delay 2 seconds
@00:54:46.16 AS.reconfigure ASconfig
@00:54:46.21 AS.seek 0.5ml
@00:55:15.22 delay 1 second
@00:55:16.24 AFV.select :holdingCoil
@00:55:16.35 ATV.dial ASV
@00:55:16.36 ASV.dial :SPErecovery
@00:55:17.01 AS.reconfigure ASslow0
@00:55:17.05 AS.seek 0.3ml
@00:55:37.47 ATV.dial :air2
@00:55:38.56 AS.reconfigure ASslow1
@00:55:38.60 AS.seek 0.32ml
@00:55:43.02 ATV.dial ASV
@00:55:44.12 AS.reconfigure ASslow0
@00:55:44.16 AS.seek 0.57ml
@00:56:09.64 ASV.dial :wasteD
@00:56:10.24 AS.reconfigure ASnorm
@00:56:10.28 AS.seek 0ml
@00:56:39.76 ATV.dial :air2
@00:56:40.80 AS.reconfigure ASslow0
@00:56:40.84 AS.seek 0.3ml
@00:57:11.33 ATV.dial :ASV
@00:57:12.37 ASV.dial :wasteD
@00:57:12.39 AS.reconfigure ASconfig
@00:57:12.43 AS.seek 0.05ml
@00:57:27.11 ATV.dial :waste
@00:57:29.12 AS.seek 0ml
@00:57:32.29 ASV.dial :SPEdelivery
@00:57:33.46 Cleaning PCR
@00:57:33.48 AFV.select :carrierSolution
@00:57:33.60 delay 2 seconds
@00:57:35.65 AS.seek 0.5ml
@00:58:04.67 delay 1 second
@00:58:05.69 AFV.select :holdingCoil
@00:58:05.80 ASV.dial :waste1
@00:58:06.17 ATV.dial :air3
@00:58:06.89 AS.reconfigure ASslow1
@00:58:06.93 AS.seek 0.51ml
@00:58:09.20 delay 2 seconds
@00:58:11.23 ATV.dial :bleach
@00:58:11.68 AS.seek 0.81ml
@00:59:12.25 delay 3 seconds
@00:59:15.28 ATV.dial :air3
@00:59:15.86 delay 3 seconds
@00:59:18.93 AS.seek 0.835ml
@00:59:24.29 delay 3 seconds
@00:59:27.32 ATV.dial :bleach
@00:59:27.95 AS.seek 0.82ml
@00:59:31.17 delay 3 seconds
@00:59:34.20 ARV.dial :waste
@00:59:34.81 delay 3 seconds
@00:59:37.84 ATV.dial :ARV
@00:59:38.76 AS.reconfigure ASconfig
@00:59:38.80 AS.seek 0.47ml
@00:59:59.17 AFV.select :carrierSolution
@00:59:59.28 delay 2 seconds
@01:00:01.30 AS.seek 1ml
@01:00:32.06 delay 1 second
@01:00:33.08 AFV.select :holdingCoil
@01:00:33.19 delay 1 second
@01:00:34.21 AS.reconfigure ASfast
@01:00:34.26 AS.seek 0ml
@01:01:01.56 AFV.select :carrierSolution
@01:01:01.67 delay 2 seconds
@01:01:03.69 AS.reconfigure ASconfig
@01:01:03.74 AS.seek 1ml
@01:02:01.51 delay 1 second
@01:02:02.53 AFV.select :holdingCoil
@01:02:02.64 delay 1 second
@01:02:03.66 AS.reconfigure ASfast
@01:02:03.71 AS.seek 0ml
@01:02:31.03 AFV.select :carrierSolution
@01:02:31.14 delay 2 seconds
@01:02:33.16 AS.reconfigure ASconfig
@01:02:33.21 AS.seek 1ml
@01:03:30.95 delay 1 second
@01:03:31.97 AFV.select :holdingCoil
@01:03:32.08 delay 1 second
@01:03:33.10 AS.reconfigure ASfast
@01:03:33.15 AS.seek 0ml
@01:04:00.47 ARV.dial between carrier and air1
@01:04:00.97 ATV.dial :air3
@01:04:02.32 AS.reconfigure ASslow1
@01:04:02.36 AS.seek 0.01ml
@01:04:04.75 delay 2 seconds
@01:04:06.78 ATV.dial :bleach
@01:04:07.40 AS.seek 0.81ml
@01:06:48.53 delay 3 seconds
@01:06:51.56 ATV.dial :air3
@01:06:52.14 delay 3 seconds
@01:06:55.21 AS.seek 0.835ml
@01:07:00.49 delay 3 seconds
@01:07:03.52 ATV.dial :bleach
@01:07:04.17 AS.seek 0.82ml
@01:07:07.59 delay 3 seconds
@01:07:10.62 ATV.dial PCR
@01:07:11.02 AS.reconfigure ASconfig
@01:07:11.06 AS.seek 0ml
@01:07:58.47 PCR.program :bleachProfile
@01:07:58.53 PCR program should complete in < 2:45
@01:07:58.54 PCR.run 10:00
@01:11:00.79 PCR.off
@01:11:00.82 AFV.select :carrierSolution
@01:11:00.92 delay 2 seconds
@01:11:02.94 AS.seek 1ml
@01:12:00.73 delay 1 second
@01:12:01.75 AFV.select :holdingCoil
@01:12:01.86 delay 1 second
@01:12:02.88 AS.reconfigure ASfast
@01:12:02.93 AS.seek 0ml
@01:12:30.26 delay 3 seconds
@01:12:33.28 AFV.select :carrierSolution
@01:12:33.39 delay 2 seconds
@01:12:35.41 AS.reconfigure ASconfig
@01:12:35.46 AS.seek 1ml
@01:13:33.19 delay 1 second
@01:13:34.21 AFV.select :holdingCoil
@01:13:34.32 delay 1 second
@01:13:35.34 AS.reconfigure ASfast
@01:13:35.39 AS.seek 0ml
@01:14:02.73 delay 3 seconds
@01:14:05.75 AFV.select :carrierSolution
@01:14:05.86 delay 2 seconds
@01:14:07.88 AS.reconfigure ASconfig
@01:14:07.93 AS.seek 1ml
@01:15:05.67 delay 1 second
@01:15:06.69 AFV.select :holdingCoil
@01:15:06.80 delay 1 second
@01:15:07.82 AS.reconfigure ASfast
@01:15:07.87 AS.seek 0ml
@01:15:35.19 delay 3 seconds
@01:15:38.21 AFV.select :carrierSolution
@01:15:38.32 delay 2 seconds
@01:15:40.34 AS.reconfigure ASconfig
@01:15:40.39 AS.seek 1ml
@01:16:38.17 delay 1 second
@01:16:39.19 AFV.select :holdingCoil
@01:16:39.30 delay 1 second
@01:16:40.32 AS.reconfigure ASfast
@01:16:40.37 AS.seek 0ml
@01:17:07.69 delay 3 seconds
@01:17:10.71 AFV.select :carrierSolution
@01:17:10.82 delay 2 seconds
@01:17:12.84 AS.reconfigure ASconfig
@01:17:12.89 AS.seek 1ml
@01:18:10.62 delay 1 second
@01:18:11.64 AFV.select :holdingCoil
@01:18:11.75 delay 1 second
@01:18:12.77 AS.reconfigure ASnorm
@01:18:12.82 AS.seek 0ml
@01:19:03.53 AFV.select :carrierSolution
@01:19:03.64 delay 2 seconds
@01:19:05.66 AS.reconfigure ASconfig
@01:19:05.71 AS.seek 1ml
@01:20:03.47 delay 1 second
@01:20:04.49 AFV.select :holdingCoil
@01:20:04.60 delay 1 second
@01:20:05.62 ATV.dial :waste
@01:20:06.91 AS.reconfigure ASnorm
@01:20:06.95 AS.seek 0.5ml
@01:20:32.50 ATV.dial :waste2
@01:20:33.87 AS.seek 0ml
@22:00:23.75CEST04-Apr-21 <MAIN> ESP2 v3.30 I2C Gateway v3.06 for core bus
@22:00:25.85 
Configuration for mer 7/20/20 brent@mbari.org
/home/esp/esp2/type/shallow/mer/configure.rb
@22:00:25.94 
Configuration for MFB3 by preston@mbari.org
/home/esp/esp2/type/mfb/MFB3/configure.rb
@22:00:30.65 Polling Can every 10:00
@22:00:32.45 CS.configure :default
@22:00:32.53 CS.hold
@22:00:32.63 CS.configure :default
@22:00:32.80 PS.configure :default
@22:00:32.96 SS.configure :default
@22:00:33.04 SS.hold
@22:00:33.12 SS.configure :default
@22:00:33.20 CC.configure :default
@22:00:33.24 CC.hold
@22:00:33.30 CC.configure :default
@22:00:33.38 PC.configure :default
@22:00:33.42 PC.hold
@22:00:33.47 PC.configure :default
@22:00:33.56 Forearm.configure :loose
@22:00:33.60 Forearm.hold
@22:00:33.66 Forearm.configure :loose
@22:00:33.74 Elbow.configure :loose
@22:00:33.78 Elbow.hold
@22:00:33.83 Elbow.configure :loose
@22:00:33.94 SC.configure :loose
@22:00:33.97 SC.hold
@22:00:34.02 SC.configure :loose
@22:00:34.10 SE.configure :default
@22:00:34.14 SE.hold
@22:00:34.20 SE.configure :default
@22:00:34.24 CH.coast
@22:00:34.28 CH.configure :default
@22:00:34.31 PH.coast
@22:00:34.36 PH.configure :default
@22:00:34.46 SPE.coast
@22:00:34.50 SPE.configure SPEconfig
@22:00:34.58 AS.configure ASconfig
@22:00:34.61 AS.hold
@22:00:34.67 AS.configure ASconfig
@22:00:34.72 PCR.off
@22:00:34.75 PCR.pidtemp= 50,0.1,0,300,25,25
@22:00:34.78 PCR.caltemp= 2.5051,0.0372,0
@22:00:34.81 All dwarf controllers running firmware version 3.75
@22:00:35.01 Cleanly exited at 21:54:04CEST04-Apr-21
@22:00:35.04 Loading /home/esp/esp2/mission/GENOMAP_v2.rb mission
@22:00:35.09 Cleaning PCR
@22:00:35.10 AFV.select :carrierSolution
@22:00:35.20 delay 2 seconds
@22:00:37.22 AS.seek 0.5ml
@22:00:39.56 <mwj> Session Begun
@22:00:43.16 -> showlog 0
@22:00:58.17 -> showlog
@22:01:06.26 <MAIN> delay 1 second
@22:01:07.28 AFV.select :holdingCoil
@22:01:07.39 ASV.dial :waste1
@22:01:07.47 ATV.dial :air3
@22:01:09.62 AS.reconfigure ASslow1
@22:01:09.68 AS.seek 0.51ml
@22:01:11.96 delay 2 seconds
@22:01:13.98 ATV.dial :bleach
@22:01:14.46 AS.seek 0.81ml
@22:02:15.05 delay 3 seconds
@22:02:18.08 ATV.dial :air3
@22:02:18.69 delay 3 seconds
@22:02:21.76 AS.seek 0.835ml
@22:02:27.30 delay 3 seconds
@22:02:30.33 ATV.dial :bleach
@22:02:30.96 AS.seek 0.82ml
@22:02:34.23 delay 3 seconds
@22:02:37.26 ARV.dial :waste
@22:02:37.76 delay 3 seconds
@22:02:40.79 ATV.dial :ARV
@22:02:41.73 AS.reconfigure ASconfig
@22:02:41.78 AS.seek 0.47ml
@22:03:02.17 AFV.select :carrierSolution
@22:03:02.29 delay 2 seconds
@22:03:04.31 AS.seek 1ml
@22:03:35.08 delay 1 second
@22:03:36.11 AFV.select :holdingCoil
@22:03:36.21 delay 1 second
@22:03:37.23 AS.reconfigure ASfast
@22:03:37.28 AS.seek 0ml
@22:04:04.59 AFV.select :carrierSolution
@22:04:04.71 delay 2 seconds
@22:04:06.73 AS.reconfigure ASconfig
@22:04:06.79 AS.seek 1ml
@22:05:04.56 delay 1 second
@22:05:05.58 AFV.select :holdingCoil
@22:05:05.68 delay 1 second
@22:05:06.70 AS.reconfigure ASfast
@22:05:06.76 AS.seek 0ml
@22:05:25.58 <mwj> -> exit
@22:05:25.59 Session Ended
@22:05:34.11 <MAIN> AFV.select :carrierSolution
@22:05:34.22 delay 2 seconds
@22:05:35.79 <mwj> Session Begun
@22:05:36.57 <MAIN> AS.reconfigure ASconfig
@22:05:36.68 AS.seek 1ml
@22:05:56.41 <mwj> -> exit
@22:05:56.42 Session Ended
@22:06:34.44 <MAIN> delay 1 second
@22:06:35.46 AFV.select :holdingCoil
@22:06:35.57 delay 1 second
@22:06:36.59 AS.reconfigure ASfast
@22:06:36.64 AS.seek 0ml
@22:07:03.95 ARV.dial between carrier and air1
@22:07:04.48 ATV.dial :air3
@22:07:05.82 AS.reconfigure ASslow1
@22:07:05.86 AS.seek 0.01ml
@22:07:08.23 delay 2 seconds
@22:07:10.26 ATV.dial :bleach
@22:07:10.89 AS.seek 0.81ml
@22:09:52.03 delay 3 seconds
@22:09:55.06 ATV.dial :air3
@22:09:55.62 delay 3 seconds
@22:09:58.69 AS.seek 0.835ml
@22:10:04.06 delay 3 seconds
@22:10:07.09 ATV.dial :bleach
@22:10:07.71 AS.seek 0.82ml
@22:10:11.11 delay 3 seconds
@22:10:14.14 ATV.dial PCR
@22:10:14.54 AS.reconfigure ASconfig
@22:10:14.58 AS.seek 0ml
@22:11:01.98 PCR.program :bleachProfile
@22:11:02.04 PCR program should complete in < 2:45
@22:11:02.05 PCR.run 10:00
@22:14:03.89 PCR.off
@22:14:03.91 AFV.select :carrierSolution
@22:14:04.02 delay 2 seconds
@22:14:06.04 AS.seek 1ml
@22:15:03.81 delay 1 second
@22:15:04.82 AFV.select :holdingCoil
@22:15:04.93 delay 1 second
@22:15:05.95 AS.reconfigure ASfast
@22:15:05.99 AS.seek 0ml
@22:15:33.31 delay 3 seconds
@22:15:36.33 AFV.select :carrierSolution
@22:15:36.44 delay 2 seconds
@22:15:38.46 AS.reconfigure ASconfig
@22:15:38.51 AS.seek 1ml
@22:16:36.25 delay 1 second
@22:16:37.26 AFV.select :holdingCoil
@22:16:37.37 delay 1 second
@22:16:38.39 AS.reconfigure ASfast
@22:16:38.44 AS.seek 0ml
@22:17:05.78 delay 3 seconds
@22:17:08.80 AFV.select :carrierSolution
@22:17:08.91 delay 2 seconds
@22:17:10.93 AS.reconfigure ASconfig
@22:17:10.98 AS.seek 1ml
@22:18:08.75 delay 1 second
@22:18:09.76 AFV.select :holdingCoil
@22:18:09.87 delay 1 second
@22:18:10.89 AS.reconfigure ASfast
@22:18:10.94 AS.seek 0ml
@22:18:38.26 delay 3 seconds
@22:18:41.28 AFV.select :carrierSolution
@22:18:41.39 delay 2 seconds
@22:18:43.41 AS.reconfigure ASconfig
@22:18:43.46 AS.seek 1ml
@22:19:41.20 delay 1 second
@22:19:42.21 AFV.select :holdingCoil
@22:19:42.32 delay 1 second
@22:19:43.34 AS.reconfigure ASfast
@22:19:43.39 AS.seek 0ml
@22:20:10.72 delay 3 seconds
@22:20:13.74 AFV.select :carrierSolution
@22:20:13.85 delay 2 seconds
@22:20:15.87 AS.reconfigure ASconfig
@22:20:15.93 AS.seek 1ml
@22:21:13.67 delay 1 second
@22:21:14.69 AFV.select :holdingCoil
@22:21:14.79 delay 1 second
@22:21:15.81 AS.reconfigure ASnorm
@22:21:15.86 AS.seek 0ml
@22:22:06.60 Cleaning PCR
AFV.select :carrierSolution
@22:22:06.70 delay 2 seconds
@22:22:08.72 AS.reconfigure ASconfig
@22:22:08.77 AS.seek 0.5ml
@22:22:37.78 delay 1 second
@22:22:38.79 AFV.select :holdingCoil
@22:22:38.90 ASV.dial :waste1
@22:22:38.91 ATV.dial :air3
@22:22:39.79 AS.reconfigure ASslow1
@22:22:39.83 AS.seek 0.51ml
@22:22:42.20 delay 2 seconds
@22:22:44.22 ATV.dial :bleach
@22:22:44.84 AS.seek 0.81ml
@22:23:45.42 delay 3 seconds
@22:23:48.45 ATV.dial :air3
@22:23:49.01 delay 3 seconds
@22:23:52.08 AS.seek 0.835ml
@22:23:57.38 delay 3 seconds
@22:24:00.41 ATV.dial :bleach
@22:24:01.04 AS.seek 0.82ml
@22:24:04.34 delay 3 seconds
@22:24:07.37 ARV.dial :waste
@22:24:07.87 delay 3 seconds
@22:24:10.90 ATV.dial :ARV
@22:24:12.18 AS.reconfigure ASconfig
@22:24:12.22 AS.seek 0.47ml
@22:24:32.61 AFV.select :carrierSolution
@22:24:32.72 delay 2 seconds
@22:24:34.74 AS.seek 1ml
@22:25:05.51 delay 1 second
@22:25:06.53 AFV.select :holdingCoil
@22:25:06.64 delay 1 second
@22:25:07.66 AS.reconfigure ASfast
@22:25:07.71 AS.seek 0ml
@22:25:35.03 AFV.select :carrierSolution
@22:25:35.14 delay 2 seconds
@22:25:37.16 AS.reconfigure ASconfig
@22:25:37.21 AS.seek 1ml
@22:26:34.95 delay 1 second
@22:26:35.97 AFV.select :holdingCoil
@22:26:36.08 delay 1 second
@22:26:37.10 AS.reconfigure ASfast
@22:26:37.15 AS.seek 0ml
@22:27:04.48 AFV.select :carrierSolution
@22:27:04.59 delay 2 seconds
@22:27:06.61 AS.reconfigure ASconfig
@22:27:06.66 AS.seek 1ml
@22:28:04.44 delay 1 second
@22:28:05.45 AFV.select :holdingCoil
@22:28:05.56 delay 1 second
@22:28:06.58 AS.reconfigure ASfast
@22:28:06.63 AS.seek 0ml
@22:28:33.95 ARV.dial between carrier and air1
@22:28:34.48 ATV.dial :air3
@22:28:35.82 AS.reconfigure ASslow1
@22:28:35.86 AS.seek 0.01ml
@22:28:38.25 delay 2 seconds
@22:28:40.28 ATV.dial :bleach
@22:28:40.90 AS.seek 0.81ml
@22:31:22.01 delay 3 seconds
@22:31:25.04 ATV.dial :air3
@22:31:25.61 delay 3 seconds
@22:31:28.68 AS.seek 0.835ml
@22:31:33.95 delay 3 seconds
@22:31:36.98 ATV.dial :bleach
@22:31:37.61 AS.seek 0.82ml
@22:31:40.95 delay 3 seconds
@22:31:43.98 ATV.dial PCR
@22:31:44.38 AS.reconfigure ASconfig
@22:31:44.42 AS.seek 0ml
@22:32:31.81 PCR.program :bleachProfile
@22:32:31.87 PCR program should complete in < 2:45
@22:32:31.88 PCR.run 10:00
@22:35:33.74 PCR.off
@22:35:33.76 AFV.select :carrierSolution
@22:35:33.87 delay 2 seconds
@22:35:35.89 AS.seek 1ml
@22:36:33.64 delay 1 second
@22:36:34.65 AFV.select :holdingCoil
@22:36:34.76 delay 1 second
@22:36:35.78 AS.reconfigure ASfast
@22:36:35.83 AS.seek 0ml
@22:37:03.17 delay 3 seconds
@22:37:06.19 AFV.select :carrierSolution
@22:37:06.30 delay 2 seconds
@22:37:08.32 AS.reconfigure ASconfig
@22:37:08.37 AS.seek 1ml
@22:38:06.11 delay 1 second
@22:38:07.12 AFV.select :holdingCoil
@22:38:07.23 delay 1 second
@22:38:08.25 AS.reconfigure ASfast
@22:38:08.30 AS.seek 0ml
@22:38:35.64 delay 3 seconds
@22:38:38.66 AFV.select :carrierSolution
@22:38:38.77 delay 2 seconds
@22:38:40.79 AS.reconfigure ASconfig
@22:38:40.84 AS.seek 1ml
@22:39:38.58 delay 1 second
@22:39:39.60 AFV.select :holdingCoil
@22:39:39.71 delay 1 second
@22:39:40.73 AS.reconfigure ASfast
@22:39:40.78 AS.seek 0ml
@22:40:08.09 delay 3 seconds
@22:40:11.11 AFV.select :carrierSolution
@22:40:11.22 delay 2 seconds
@22:40:13.24 AS.reconfigure ASconfig
@22:40:13.29 AS.seek 1ml
@22:41:11.06 delay 1 second
@22:41:12.07 AFV.select :holdingCoil
@22:41:12.18 delay 1 second
@22:41:13.20 AS.reconfigure ASfast
@22:41:13.25 AS.seek 0ml
@22:41:40.56 delay 3 seconds
@22:41:43.58 AFV.select :carrierSolution
@22:41:43.69 delay 2 seconds
@22:41:45.71 AS.reconfigure ASconfig
@22:41:45.76 AS.seek 1ml
@22:42:43.55 delay 1 second
@22:42:44.56 AFV.select :holdingCoil
@22:42:44.66 delay 1 second
@22:42:45.68 AS.reconfigure ASnorm
@22:42:45.73 AS.seek 0ml
@22:43:36.45 Cleaning PCR
@22:43:36.46 AFV.select :carrierSolution
@22:43:36.56 delay 2 seconds
@22:43:38.58 AS.reconfigure ASconfig
@22:43:38.63 AS.seek 0.5ml
@22:44:07.70 delay 1 second
@22:44:08.71 AFV.select :holdingCoil
@22:44:08.82 ASV.dial :waste1
@22:44:08.83 ATV.dial :air3
@22:44:09.70 AS.reconfigure ASslow1
@22:44:09.74 AS.seek 0.51ml
@22:44:12.00 delay 2 seconds
@22:44:14.32 ATV.dial :bleach
@22:44:15.00 AS.seek 0.81ml
@22:45:15.67 delay 3 seconds
@22:45:18.70 ATV.dial :air3
@22:45:19.26 delay 3 seconds
@22:45:22.33 AS.seek 0.835ml
@22:45:27.63 delay 3 seconds
@22:45:30.66 ATV.dial :bleach
@22:45:31.29 AS.seek 0.82ml
@22:45:34.56 delay 3 seconds
@22:45:37.59 ARV.dial :waste
@22:45:38.09 delay 3 seconds
@22:45:41.12 ATV.dial :ARV
@22:45:42.03 AS.reconfigure ASconfig
@22:45:42.07 AS.seek 0.47ml
@22:46:02.47 AFV.select :carrierSolution
@22:46:02.58 delay 2 seconds
@22:46:04.60 AS.seek 1ml
@22:46:35.37 delay 1 second
@22:46:36.38 AFV.select :holdingCoil
@22:46:36.49 delay 1 second
@22:46:37.51 AS.reconfigure ASfast
@22:46:37.56 AS.seek 0ml
@22:47:04.89 AFV.select :carrierSolution
@22:47:05.00 delay 2 seconds
@22:47:07.02 AS.reconfigure ASconfig
@22:47:07.07 AS.seek 1ml
@22:48:04.83 delay 1 second
@22:48:05.84 AFV.select :holdingCoil
@22:48:05.95 delay 1 second
@22:48:06.97 AS.reconfigure ASfast
@22:48:07.02 AS.seek 0ml
@22:48:34.36 AFV.select :carrierSolution
@22:48:34.47 delay 2 seconds
@22:48:36.49 AS.reconfigure ASconfig
@22:48:36.54 AS.seek 1ml
@22:49:34.31 delay 1 second
@22:49:35.33 AFV.select :holdingCoil
@22:49:35.44 delay 1 second
@22:49:36.46 AS.reconfigure ASfast
@22:49:36.51 AS.seek 0ml
@22:50:03.84 ARV.dial between carrier and air1
@22:50:04.36 ATV.dial :air3
@22:50:05.70 AS.reconfigure ASslow1
@22:50:05.74 AS.seek 0.01ml
@22:50:08.00 delay 2 seconds
@22:50:10.03 ATV.dial :bleach
@22:50:10.65 AS.seek 0.81ml
@22:52:51.78 delay 3 seconds
@22:52:54.81 ATV.dial :air3
@22:52:55.37 delay 3 seconds
@22:52:58.44 AS.seek 0.835ml
@22:53:03.88 delay 3 seconds
@22:53:06.91 ATV.dial :bleach
@22:53:07.53 AS.seek 0.82ml
@22:53:10.81 delay 3 seconds
@22:53:13.84 ATV.dial PCR
@22:53:14.26 AS.reconfigure ASconfig
@22:53:14.30 AS.seek 0ml
@22:54:01.70 PCR.program :bleachProfile
@22:54:01.76 PCR program should complete in < 2:45
@22:54:01.77 PCR.run 10:00
@22:57:03.61 PCR.off
@22:57:03.63 AFV.select :carrierSolution
@22:57:03.74 delay 2 seconds
@22:57:05.76 AS.seek 1ml
@22:58:03.50 delay 1 second
@22:58:04.52 AFV.select :holdingCoil
@22:58:04.63 delay 1 second
@22:58:05.65 AS.reconfigure ASfast
@22:58:05.70 AS.seek 0ml
@22:58:33.03 delay 3 seconds
@22:58:36.05 AFV.select :carrierSolution
@22:58:36.16 delay 2 seconds
@22:58:38.18 AS.reconfigure ASconfig
@22:58:38.23 AS.seek 1ml
@22:59:35.97 delay 1 second
@22:59:36.98 AFV.select :holdingCoil
@22:59:37.09 delay 1 second
@22:59:38.11 AS.reconfigure ASfast
@22:59:38.16 AS.seek 0ml
@23:00:05.50 delay 3 seconds
@23:00:08.52 AFV.select :carrierSolution
@23:00:08.63 delay 2 seconds
@23:00:10.65 AS.reconfigure ASconfig
@23:00:10.70 AS.seek 1ml
@23:01:08.44 delay 1 second
@23:01:09.45 AFV.select :holdingCoil
@23:01:09.56 delay 1 second
@23:01:10.58 AS.reconfigure ASfast
@23:01:10.63 AS.seek 0ml
@23:01:37.95 delay 3 seconds
@23:01:40.97 AFV.select :carrierSolution
@23:01:41.08 delay 2 seconds
@23:01:43.10 AS.reconfigure ASconfig
@23:01:43.15 AS.seek 1ml
@23:02:40.87 delay 1 second
@23:02:41.88 AFV.select :holdingCoil
@23:02:41.99 delay 1 second
@23:02:43.01 AS.reconfigure ASfast
@23:02:43.06 AS.seek 0ml
@23:03:10.39 delay 3 seconds
@23:03:13.41 AFV.select :carrierSolution
@23:03:13.52 delay 2 seconds
@23:03:15.54 AS.reconfigure ASconfig
@23:03:15.60 AS.seek 1ml
@23:04:13.69 delay 1 second
@23:04:14.70 AFV.select :holdingCoil
@23:04:14.81 delay 1 second
@23:04:15.83 AS.reconfigure ASnorm
@23:04:15.88 AS.seek 0ml
@23:05:06.70 <MFBPCR> Setting up PCR for ACYPP2 -- writing /var/log/esp/ACYPP221apr0423h.pcr
@23:05:06.72 Building PCR Slug from elutionWater with MM1 mix, PP2 primer
@23:05:06.73 Priming with carrier
@23:05:06.74 ATV.dial ARV
@23:05:07.36 ARV.dial :carrier
@23:05:07.70 AS.seek 0.5ml
@23:05:33.22 AFV.select :carrierSolution
@23:05:33.33 delay 2 seconds
@23:05:35.35 AS.reconfigure ASconfig
@23:05:35.40 AS.seek 1ml
@23:06:04.41 delay 1 second
@23:06:05.43 AFV.select :holdingCoil
@23:06:05.54 delay 1 second
@23:06:06.56 ATV.dial :waste
@23:06:08.32 AS.reconfigure ASnorm
@23:06:08.36 AS.seek 0ml
@23:06:59.05 AFV.select :carrierSolution
@23:06:59.16 delay 2 seconds
@23:07:01.18 AS.reconfigure ASconfig
@23:07:01.23 AS.seek 0.5ml
@23:07:30.26 delay 1 second
@23:07:31.28 AFV.select :holdingCoil
@23:07:31.39 Building Slug
@23:07:31.40 ATV.dial :ARV
@23:07:33.12 delay 2 seconds
@23:07:35.15 ARV.dial :air1
@23:07:35.54 delay 2 seconds
@23:07:37.61 AS.reconfigure ASslow2
@23:07:37.66 AS.seek 0.506ml
@23:07:44.06 delay 2 seconds
@23:07:46.09 ARV.dial :MM1
@23:07:46.72 delay 2 seconds
@23:07:48.75 Adding MM1
@23:07:48.81 AS.seek 0.524ml
@23:08:07.59 delay 25 seconds
@23:08:32.62 ARV.dial :PP2
@23:08:33.02 Adding PP2
@23:08:33.03 delay 2 seconds
@23:08:35.09 AS.seek 0.53ml
@23:08:41.64 delay 10 seconds
@23:08:51.68 ARV.dial :air2
@23:08:52.09 adding ARV air
@23:08:52.10 delay 2 seconds
@23:08:54.16 AS.seek 0.536ml
@23:09:00.50 delay 5 seconds
@23:09:05.53 ARV.dial :carrier
@23:09:06.87 delay 7 seconds
@23:09:13.94 AS.seek 0.594ml
@23:10:12.61 delay 5 seconds
@23:10:17.64 ATV.dial :elutionWater
@23:10:19.16 delay 2 seconds
@23:10:21.23 AS.seek 0.6ml
@23:10:27.64 delay 4 seconds
@23:10:31.67 ATV.dial ARV
@23:10:33.16 delay 2 seconds
@23:10:35.19 ARV.dial :carrier
@23:10:35.20 delay 2 seconds
@23:10:37.26 AS.seek 0.65ml
@23:11:27.78 delay 4 seconds
@23:11:31.81 ATV.dial PCR
@23:11:32.59 ASV.dial :waste1
@23:11:32.61 ARV.dial :air1
@23:11:33.02 Positioning Slug by 0.324ml
@23:11:33.09 AS.seek 0.326ml
@23:16:59.47 delay 5 seconds
@23:17:04.50 ATV.dial ARV
@23:17:05.28 ASV.dial :PCRlock
@23:17:05.67 delay 5 seconds
@23:17:10.70 Running ACYPP2 Profile60CIQ2 thermocycling
@23:17:10.71 <PCR> PCR.program Profile60CIQ2
@23:17:10.82 Profile60CIQ2 should complete in < 1:47:50
@23:17:10.83 PCR.run 2:19:47.5
@00:45:48.26CEST05-Apr-21 PCR.off
@00:45:48.88 <MFBPCR> Running ACYPP2 MeltProfile60 thermocycling
@00:45:48.90 <PCR> PCR.program MeltProfile60
@00:45:48.99 MeltProfile60 should complete in < 11:55
@00:45:49.00 PCR.run 19:53.75
@00:53:26.51 PCR.off
@00:53:27.45 <MFBPCR> PCR for ACYPP2 completed -- closed /var/log/esp/ACYPP221apr0423h.pcr
@00:53:27.51 Cleaning PCR
AFV.select :carrierSolution
@00:53:27.62 delay 2 seconds
@00:53:29.64 AS.reconfigure ASconfig
@00:53:29.69 AS.seek 0.5ml
@00:53:39.98 delay 1 second
@00:53:41.00 AFV.select :holdingCoil
@00:53:41.11 ASV.dial :waste1
@00:53:41.48 ATV.dial :air3
@00:53:42.83 AS.reconfigure ASslow1
@00:53:42.87 AS.seek 0.51ml
@00:53:45.23 delay 2 seconds
@00:53:47.58 ATV.dial :bleach
@00:53:48.25 AS.seek 0.81ml
@00:54:48.84 delay 3 seconds
@00:54:51.87 ATV.dial :air3
@00:54:52.44 delay 3 seconds
@00:54:55.51 AS.seek 0.835ml
@00:55:00.90 delay 3 seconds
@00:55:03.93 ATV.dial :bleach
@00:55:04.56 AS.seek 0.82ml
@00:55:07.83 delay 3 seconds
@00:55:10.86 ARV.dial :waste
@00:55:11.48 delay 3 seconds
@00:55:14.51 ATV.dial :ARV
@00:55:15.42 AS.reconfigure ASconfig
@00:55:15.46 AS.seek 0.47ml
@00:55:35.83 AFV.select :carrierSolution
@00:55:35.94 delay 2 seconds
@00:55:37.96 AS.seek 1ml
@00:56:08.73 delay 1 second
@00:56:09.75 AFV.select :holdingCoil
@00:56:09.86 delay 1 second
@00:56:10.88 AS.reconfigure ASfast
@00:56:10.93 AS.seek 0ml
@00:56:38.22 AFV.select :carrierSolution
@00:56:38.33 delay 2 seconds
@00:56:40.35 AS.reconfigure ASconfig
@00:56:40.40 AS.seek 1ml
@00:57:38.12 delay 1 second
@00:57:39.14 AFV.select :holdingCoil
@00:57:39.25 delay 1 second
@00:57:40.27 AS.reconfigure ASfast
@00:57:40.32 AS.seek 0ml
@00:58:07.62 AFV.select :carrierSolution
@00:58:07.73 delay 2 seconds
@00:58:09.75 AS.reconfigure ASconfig
@00:58:09.80 AS.seek 1ml
@00:59:07.55 delay 1 second
@00:59:08.57 AFV.select :holdingCoil
@00:59:08.68 delay 1 second
@00:59:09.70 AS.reconfigure ASfast
@00:59:09.75 AS.seek 0ml
@00:59:37.08 ARV.dial between carrier and air1
@00:59:37.60 ATV.dial :air3
@00:59:38.93 AS.reconfigure ASslow1
@00:59:38.97 AS.seek 0.01ml
@00:59:41.37 delay 2 seconds
@00:59:43.40 ATV.dial :bleach
@00:59:44.03 AS.seek 0.81ml
@01:02:25.19 delay 3 seconds
@01:02:28.22 ATV.dial :air3
@01:02:28.78 delay 3 seconds
@01:02:31.85 AS.seek 0.835ml
@01:02:37.27 delay 3 seconds
@01:02:40.30 ATV.dial :bleach
@01:02:40.92 AS.seek 0.82ml
@01:02:44.34 delay 3 seconds
@01:02:47.37 ATV.dial PCR
@01:02:47.78 AS.reconfigure ASconfig
@01:02:47.82 AS.seek 0ml
@01:03:35.23 PCR.program :bleachProfile
@01:03:35.29 PCR program should complete in < 2:45
@01:03:35.30 PCR.run 10:00
@01:06:31.45 PCR.off
@01:06:31.47 AFV.select :carrierSolution
@01:06:31.58 delay 2 seconds
@01:06:33.60 AS.seek 1ml
@01:07:31.34 delay 1 second
@01:07:32.36 AFV.select :holdingCoil
@01:07:32.47 delay 1 second
@01:07:33.49 AS.reconfigure ASfast
@01:07:33.54 AS.seek 0ml
@01:08:00.87 delay 3 seconds
@01:08:03.89 AFV.select :carrierSolution
@01:08:04.00 delay 2 seconds
@01:08:06.03 AS.reconfigure ASconfig
@01:08:06.08 AS.seek 1ml
@01:09:03.84 delay 1 second
@01:09:04.86 AFV.select :holdingCoil
@01:09:04.97 delay 1 second
@01:09:05.99 AS.reconfigure ASfast
@01:09:06.04 AS.seek 0ml
@01:09:33.34 delay 3 seconds
@01:09:36.36 AFV.select :carrierSolution
@01:09:36.47 delay 2 seconds
@01:09:38.49 AS.reconfigure ASconfig
@01:09:38.54 AS.seek 1ml
@01:10:36.27 delay 1 second
@01:10:37.29 AFV.select :holdingCoil
@01:10:37.40 delay 1 second
@01:10:38.42 AS.reconfigure ASfast
@01:10:38.47 AS.seek 0ml
@01:11:05.80 delay 3 seconds
@01:11:08.82 AFV.select :carrierSolution
@01:11:08.93 delay 2 seconds
@01:11:10.95 AS.reconfigure ASconfig
@01:11:11.01 AS.seek 1ml
@01:12:08.75 delay 1 second
@01:12:09.77 AFV.select :holdingCoil
@01:12:09.88 delay 1 second
@01:12:10.90 AS.reconfigure ASfast
@01:12:10.95 AS.seek 0ml
@01:12:38.27 delay 3 seconds
@01:12:41.29 AFV.select :carrierSolution
@01:12:41.40 delay 2 seconds
@01:12:43.42 AS.reconfigure ASconfig
@01:12:43.47 AS.seek 1ml
@01:13:41.27 delay 1 second
@01:13:42.29 AFV.select :holdingCoil
@01:13:42.40 delay 1 second
@01:13:43.42 AS.reconfigure ASnorm
@01:13:43.47 AS.seek 0ml
@01:14:34.23 Setting up PCR for AOPP1 -- writing /var/log/esp/AOPP121apr0501h.pcr
@01:14:34.24 Building PCR Slug from elutionWater with MM1 mix, PP1 primer
@01:14:34.26 Priming with carrier
ATV.dial ARV
@01:14:34.87 ARV.dial :carrier
@01:14:35.22 AS.seek 0.5ml
@01:15:00.75 AFV.select :carrierSolution
@01:15:00.86 delay 2 seconds
@01:15:02.88 AS.reconfigure ASconfig
@01:15:02.93 AS.seek 1ml
@01:15:31.92 delay 1 second
@01:15:32.94 AFV.select :holdingCoil
@01:15:33.05 delay 1 second
@01:15:34.07 ATV.dial :waste
@01:15:35.84 AS.reconfigure ASnorm
@01:15:35.88 AS.seek 0ml
@01:16:26.55 AFV.select :carrierSolution
@01:16:26.66 delay 2 seconds
@01:16:28.68 AS.reconfigure ASconfig
@01:16:28.73 AS.seek 0.5ml
@01:16:57.73 delay 1 second
@01:16:58.75 AFV.select :holdingCoil
@01:16:58.86 Building Slug
@01:16:58.87 ATV.dial :ARV
@01:17:00.59 delay 2 seconds
@01:17:02.62 ARV.dial :air1
@01:17:03.03 delay 2 seconds
@01:17:05.10 AS.reconfigure ASslow2
@01:17:05.15 AS.seek 0.506ml
@01:17:11.42 delay 2 seconds
@01:17:13.45 ARV.dial :MM1
@01:17:14.06 delay 2 seconds
@01:17:16.09 Adding MM1
@01:17:16.14 AS.seek 0.524ml
@01:17:34.64 delay 25 seconds
@01:17:59.67 ARV.dial :PP1
@01:18:00.07 Adding PP1
@01:18:00.08 delay 2 seconds
@01:18:02.14 AS.seek 0.53ml
@01:18:08.33 delay 10 seconds
@01:18:18.37 ARV.dial :air1
@01:18:18.76 adding ARV air
@01:18:18.77 delay 2 seconds
@01:18:20.83 AS.seek 0.536ml
@01:18:27.09 delay 5 seconds
@01:18:32.12 ARV.dial :carrier
@01:18:32.53 delay 7 seconds
@01:18:39.60 AS.seek 0.594ml
@01:19:38.02 delay 5 seconds
@01:19:43.05 ATV.dial :elutionWater
@01:19:44.56 delay 2 seconds
@01:19:46.63 AS.seek 0.6ml
@01:19:52.87 delay 4 seconds
@01:19:56.90 ATV.dial ARV
@01:19:58.37 delay 2 seconds
@01:20:00.40 ARV.dial :carrier
@01:20:00.41 delay 2 seconds
@01:20:02.47 AS.seek 0.65ml
@01:20:53.11 delay 4 seconds
@01:20:57.14 ATV.dial PCR
@01:20:57.92 ASV.dial :waste1
@01:20:57.94 ARV.dial :air1
@01:20:58.32 Positioning Slug by 0.324ml
@01:20:58.39 AS.seek 0.326ml
@01:26:24.33 delay 5 seconds
@01:26:29.36 ATV.dial ARV
@01:26:30.25 ASV.dial :PCRlock
@01:26:30.64 delay 5 seconds
@01:26:35.67 Running AOPP1 Profile60CIQ1 thermocycling
@01:26:35.68 <PCR> PCR.program Profile60CIQ1
@01:26:35.79 Profile60CIQ1 should complete in < 1:56:15
@01:26:36.15 PCR.run 2:30:18.75
@03:05:45.35 PCR.off
@03:05:45.90 <MFBPCR> Running AOPP1 MeltProfile60 thermocycling
@03:05:45.91 <PCR> PCR.program MeltProfile60
@03:05:46.00 MeltProfile60 should complete in < 11:55
@03:05:46.01 PCR.run 19:53.75
@03:13:14.48 PCR.off
@03:13:15.68 <MFBPCR> PCR for AOPP1 completed -- closed /var/log/esp/AOPP121apr0501h.pcr
@03:13:15.74 Cleaning PCR
@03:13:15.75 AFV.select :carrierSolution
@03:13:15.86 delay 2 seconds
@03:13:17.88 AS.reconfigure ASconfig
@03:13:17.93 AS.seek 0.5ml
@03:13:28.22 delay 1 second
@03:13:29.24 AFV.select :holdingCoil
@03:13:29.35 ASV.dial :waste1
@03:13:29.74 ATV.dial :air3
@03:13:31.08 AS.reconfigure ASslow1
@03:13:31.12 AS.seek 0.51ml
@03:13:33.42 delay 2 seconds
@03:13:35.45 ATV.dial :bleach
@03:13:36.08 AS.seek 0.81ml
@03:14:36.75 delay 3 seconds
@03:14:39.78 ATV.dial :air3
@03:14:40.34 delay 3 seconds
@03:14:43.41 AS.seek 0.835ml
@03:14:48.72 delay 3 seconds
@03:14:51.75 ATV.dial :bleach
@03:14:52.38 AS.seek 0.82ml
@03:14:55.67 delay 3 seconds
@03:14:58.70 ARV.dial :waste
@03:14:59.31 delay 3 seconds
@03:15:02.34 ATV.dial :ARV
@03:15:03.26 AS.reconfigure ASconfig
@03:15:03.30 AS.seek 0.47ml
@03:15:23.72 AFV.select :carrierSolution
@03:15:23.86 delay 2 seconds
@03:15:25.90 AS.seek 1ml
@03:15:56.64 delay 1 second
@03:15:57.66 AFV.select :holdingCoil
@03:15:57.77 delay 1 second
@03:15:58.79 AS.reconfigure ASfast
@03:15:58.84 AS.seek 0ml
@03:16:26.13 AFV.select :carrierSolution
@03:16:26.24 delay 2 seconds
@03:16:28.26 AS.reconfigure ASconfig
@03:16:28.31 AS.seek 1ml
@03:17:26.05 delay 1 second
@03:17:27.07 AFV.select :holdingCoil
@03:17:27.18 delay 1 second
@03:17:28.20 AS.reconfigure ASfast
@03:17:28.25 AS.seek 0ml
@03:17:55.58 AFV.select :carrierSolution
@03:17:55.69 delay 2 seconds
@03:17:57.71 AS.reconfigure ASconfig
@03:17:57.76 AS.seek 1ml
@03:18:55.50 delay 1 second
@03:18:56.52 AFV.select :holdingCoil
@03:18:56.63 delay 1 second
@03:18:57.65 AS.reconfigure ASfast
@03:18:57.70 AS.seek 0ml
@03:19:25.02 ARV.dial between carrier and air1
@03:19:25.52 ATV.dial :air3
@03:19:26.86 AS.reconfigure ASslow1
@03:19:26.90 AS.seek 0.01ml
@03:19:29.16 delay 2 seconds
@03:19:31.19 ATV.dial :bleach
@03:19:31.81 AS.seek 0.81ml
@03:22:12.91 delay 3 seconds
@03:22:15.94 ATV.dial :air3
@03:22:16.50 delay 3 seconds
@03:22:19.57 AS.seek 0.835ml
@03:22:24.89 delay 3 seconds
@03:22:27.92 ATV.dial :bleach
@03:22:28.55 AS.seek 0.82ml
@03:22:31.80 delay 3 seconds
@03:22:34.83 ATV.dial PCR
@03:22:35.23 AS.reconfigure ASconfig
@03:22:35.27 AS.seek 0ml
@03:23:22.67 PCR.program :bleachProfile
@03:23:22.74 PCR program should complete in < 2:45
@03:23:22.75 PCR.run 10:00
@03:26:18.94 PCR.off
@03:26:18.96 AFV.select :carrierSolution
@03:26:19.07 delay 2 seconds
@03:26:21.09 AS.seek 1ml
@03:27:18.83 delay 1 second
@03:27:19.85 AFV.select :holdingCoil
@03:27:19.96 delay 1 second
@03:27:20.98 AS.reconfigure ASfast
@03:27:21.03 AS.seek 0ml
@03:27:48.34 delay 3 seconds
@03:27:51.36 AFV.select :carrierSolution
@03:27:51.47 delay 2 seconds
@03:27:53.49 AS.reconfigure ASconfig
@03:27:53.54 AS.seek 1ml
@03:28:51.28 delay 1 second
@03:28:52.30 AFV.select :holdingCoil
@03:28:52.41 delay 1 second
@03:28:53.43 AS.reconfigure ASfast
@03:28:53.48 AS.seek 0ml
@03:29:20.81 delay 3 seconds
@03:29:23.83 AFV.select :carrierSolution
@03:29:23.94 delay 2 seconds
@03:29:25.96 AS.reconfigure ASconfig
@03:29:26.01 AS.seek 1ml
@03:30:23.75 delay 1 second
@03:30:24.77 AFV.select :holdingCoil
@03:30:24.88 delay 1 second
@03:30:25.90 AS.reconfigure ASfast
@03:30:25.95 AS.seek 0ml
@03:30:53.28 delay 3 seconds
@03:30:56.31 AFV.select :carrierSolution
@03:30:56.41 delay 2 seconds
@03:30:58.43 AS.reconfigure ASconfig
@03:30:58.48 AS.seek 1ml
@03:31:56.22 delay 1 second
@03:31:57.24 AFV.select :holdingCoil
@03:31:57.35 delay 1 second
@03:31:58.37 AS.reconfigure ASfast
@03:31:58.42 AS.seek 0ml
@03:32:25.75 delay 3 seconds
@03:32:28.77 AFV.select :carrierSolution
@03:32:28.88 delay 2 seconds
@03:32:30.90 AS.reconfigure ASconfig
@03:32:30.96 AS.seek 1ml
@03:33:28.69 delay 1 second
@03:33:29.71 AFV.select :holdingCoil
@03:33:29.82 delay 1 second
@03:33:30.84 AS.reconfigure ASnorm
@03:33:30.89 AS.seek 0ml
@03:34:21.59 Setting up PCR for AARPP4 -- writing /var/log/esp/AARPP421apr0503h.pcr
@03:34:21.60 Building PCR Slug from elutionWater with MM2 mix, PP4 primer
@03:34:21.61 Priming with carrier
@03:34:21.62 ATV.dial ARV
@03:34:22.25 ARV.dial :carrier
@03:34:22.59 AS.seek 0.5ml
@03:34:48.09 AFV.select :carrierSolution
@03:34:48.20 delay 2 seconds
@03:34:50.22 AS.reconfigure ASconfig
@03:34:50.27 AS.seek 1ml
@03:35:19.27 delay 1 second
@03:35:20.29 AFV.select :holdingCoil
@03:35:20.40 delay 1 second
@03:35:21.42 ATV.dial :waste
@03:35:23.20 AS.reconfigure ASnorm
@03:35:23.24 AS.seek 0ml
@03:36:13.92 AFV.select :carrierSolution
@03:36:14.03 delay 2 seconds
@03:36:16.05 AS.reconfigure ASconfig
@03:36:16.10 AS.seek 0.5ml
@03:36:45.16 delay 1 second
@03:36:46.18 AFV.select :holdingCoil
@03:36:46.29 Building Slug
@03:36:46.30 ATV.dial :ARV
@03:36:48.03 delay 2 seconds
@03:36:50.06 ARV.dial :air1
@03:36:50.46 delay 2 seconds
@03:36:52.53 AS.reconfigure ASslow2
@03:36:52.58 AS.seek 0.506ml
@03:36:58.83 delay 2 seconds
@03:37:00.86 ARV.dial :MM2
@03:37:02.42 delay 2 seconds
@03:37:04.45 Adding MM2
@03:37:04.51 AS.seek 0.524ml
@03:37:22.80 delay 25 seconds
@03:37:47.83 ARV.dial :PP4
@03:37:48.23 Adding PP4
@03:37:48.24 delay 2 seconds
@03:37:50.30 AS.seek 0.53ml
@03:37:57.06 delay 10 seconds
@03:38:07.10 ARV.dial :air3
@03:38:07.51 adding ARV air
@03:38:07.52 delay 2 seconds
@03:38:09.58 AS.seek 0.536ml
@03:38:16.00 delay 5 seconds
@03:38:21.03 ARV.dial :carrier
@03:38:22.33 delay 7 seconds
@03:38:29.41 AS.seek 0.594ml
@03:39:28.05 delay 5 seconds
@03:39:33.08 ATV.dial :elutionWater
@03:39:34.58 delay 2 seconds
@03:39:36.65 AS.seek 0.6ml
@03:39:42.99 delay 4 seconds
@03:39:47.02 ATV.dial ARV
@03:39:48.53 delay 2 seconds
@03:39:50.56 ARV.dial :carrier
@03:39:50.57 delay 2 seconds
@03:39:52.63 AS.seek 0.65ml
@03:40:43.50 delay 4 seconds
@03:40:47.53 ATV.dial PCR
@03:40:48.31 ASV.dial :waste1
@03:40:48.33 ARV.dial :air1
@03:40:48.71 Positioning Slug by 0.324ml
@03:40:48.79 AS.seek 0.326ml
@03:46:14.88 delay 5 seconds
@03:46:19.91 ATV.dial ARV
@03:46:20.70 ASV.dial :PCRlock
@03:46:21.10 delay 5 seconds
@03:46:26.13 Running AARPP4 Profile58CIQ2 thermocycling
@03:46:26.14 <PCR> PCR.program Profile58CIQ2
@03:46:26.25 Profile58CIQ2 should complete in < 1:36:15
@03:46:26.26 PCR.run 2:05:18.75
@05:07:26.19 PCR.off
@05:07:26.79 <MFBPCR> Running AARPP4 MeltProfile58 thermocycling
@05:07:26.80 <PCR> PCR.program MeltProfile58
@05:07:26.89 MeltProfile58 should complete in < 12:33
@05:07:26.90 PCR.run 20:41.25
@05:15:15.06 PCR.off
@05:15:16.05 <MFBPCR> PCR for AARPP4 completed -- closed /var/log/esp/AARPP421apr0503h.pcr
@05:15:16.11 Cleaning PCR
@05:15:16.12 AFV.select :carrierSolution
@05:15:16.22 delay 2 seconds
@05:15:18.24 AS.reconfigure ASconfig
@05:15:18.29 AS.seek 0.5ml
@05:15:28.60 delay 1 second
@05:15:29.62 AFV.select :holdingCoil
@05:15:29.73 ASV.dial :waste1
@05:15:30.11 ATV.dial :air3
@05:15:31.47 AS.reconfigure ASslow1
@05:15:31.51 AS.seek 0.51ml
@05:15:33.86 delay 2 seconds
@05:15:35.88 ATV.dial :bleach
@05:15:36.50 AS.seek 0.81ml
@05:16:37.10 delay 3 seconds
@05:16:40.13 ATV.dial :air3
@05:16:40.69 delay 3 seconds
@05:16:43.76 AS.seek 0.835ml
@05:16:49.17 delay 3 seconds
@05:16:52.20 ATV.dial :bleach
@05:16:52.83 AS.seek 0.82ml
@05:16:56.11 delay 3 seconds
@05:16:59.14 ARV.dial :waste
@05:16:59.75 delay 3 seconds
@05:17:02.78 ATV.dial :ARV
@05:17:03.69 AS.reconfigure ASconfig
@05:17:03.73 AS.seek 0.47ml
@05:17:24.13 AFV.select :carrierSolution
@05:17:24.24 delay 2 seconds
@05:17:26.26 AS.seek 1ml
@05:17:57.03 delay 1 second
@05:17:58.05 AFV.select :holdingCoil
@05:17:58.16 delay 1 second
@05:17:59.18 AS.reconfigure ASfast
@05:17:59.23 AS.seek 0ml
@05:18:26.55 AFV.select :carrierSolution
@05:18:26.66 delay 2 seconds
@05:18:28.68 AS.reconfigure ASconfig
@05:18:28.73 AS.seek 1ml
@05:19:26.50 delay 1 second
@05:19:27.52 AFV.select :holdingCoil
@05:19:27.63 delay 1 second
@05:19:28.65 AS.reconfigure ASfast
@05:19:28.70 AS.seek 0ml
@05:19:56.02 AFV.select :carrierSolution
@05:19:56.13 delay 2 seconds
@05:19:58.15 AS.reconfigure ASconfig
@05:19:58.20 AS.seek 1ml
@05:20:55.94 delay 1 second
@05:20:56.96 AFV.select :holdingCoil
@05:20:57.07 delay 1 second
@05:20:58.09 AS.reconfigure ASfast
@05:20:58.14 AS.seek 0ml
@05:21:25.46 ARV.dial between carrier and air1
@05:21:25.97 ATV.dial :air3
@05:21:27.31 AS.reconfigure ASslow1
@05:21:27.35 AS.seek 0.01ml
@05:21:29.63 delay 2 seconds
@05:21:31.66 ATV.dial :bleach
@05:21:32.28 AS.seek 0.81ml
@05:24:13.38 delay 3 seconds
@05:24:16.41 ATV.dial :air3
@05:24:16.99 delay 3 seconds
@05:24:20.06 AS.seek 0.835ml
@05:24:25.36 delay 3 seconds
@05:24:28.39 ATV.dial :bleach
@05:24:29.02 AS.seek 0.82ml
@05:24:32.31 delay 3 seconds
@05:24:35.34 ATV.dial PCR
@05:24:35.76 AS.reconfigure ASconfig
@05:24:35.80 AS.seek 0ml
@05:25:23.21 PCR.program :bleachProfile
@05:25:23.68 PCR program should complete in < 2:45
@05:25:23.70 PCR.run 10:00
@05:28:19.47 PCR.off
@05:28:19.49 AFV.select :carrierSolution
@05:28:19.60 delay 2 seconds
@05:28:21.62 AS.seek 1ml
@05:29:19.35 delay 1 second
@05:29:20.37 AFV.select :holdingCoil
@05:29:20.48 delay 1 second
@05:29:21.50 AS.reconfigure ASfast
@05:29:21.55 AS.seek 0ml
@05:29:48.88 delay 3 seconds
@05:29:51.90 AFV.select :carrierSolution
@05:29:52.01 delay 2 seconds
@05:29:54.03 AS.reconfigure ASconfig
@05:29:54.08 AS.seek 1ml
@05:30:51.81 delay 1 second
@05:30:52.83 AFV.select :holdingCoil
@05:30:52.94 delay 1 second
@05:30:53.96 AS.reconfigure ASfast
@05:30:54.01 AS.seek 0ml
@05:31:21.32 delay 3 seconds
@05:31:24.34 AFV.select :carrierSolution
@05:31:24.45 delay 2 seconds
@05:31:26.47 AS.reconfigure ASconfig
@05:31:26.52 AS.seek 1ml
@05:32:24.30 delay 1 second
@05:32:25.32 AFV.select :holdingCoil
@05:32:25.43 delay 1 second
@05:32:26.45 AS.reconfigure ASfast
@05:32:26.50 AS.seek 0ml
@05:32:53.81 delay 3 seconds
@05:32:56.83 AFV.select :carrierSolution
@05:32:56.94 delay 2 seconds
@05:32:58.96 AS.reconfigure ASconfig
@05:32:59.01 AS.seek 1ml
@05:33:56.75 delay 1 second
@05:33:57.77 AFV.select :holdingCoil
@05:33:57.88 delay 1 second
@05:33:58.90 AS.reconfigure ASfast
@05:33:58.95 AS.seek 0ml
@05:34:26.27 delay 3 seconds
@05:34:29.29 AFV.select :carrierSolution
@05:34:29.40 delay 2 seconds
@05:34:31.42 AS.reconfigure ASconfig
@05:34:31.47 AS.seek 1ml
@05:35:29.21 delay 1 second
@05:35:30.23 AFV.select :holdingCoil
@05:35:30.34 delay 1 second
@05:35:31.36 AS.reconfigure ASnorm
@05:35:31.41 AS.seek 0ml
@05:36:22.13 Setting up PCR for AALPP3 -- writing /var/log/esp/AALPP321apr0505h.pcr
@05:36:22.14 Building PCR Slug from elutionWater with MM2 mix, PP3 primer
@05:36:22.15 Priming with carrier
@05:36:22.16 ATV.dial ARV
@05:36:22.78 ARV.dial :carrier
@05:36:23.13 AS.seek 0.5ml
@05:36:48.64 AFV.select :carrierSolution
@05:36:48.75 delay 2 seconds
@05:36:50.77 AS.reconfigure ASconfig
@05:36:50.82 AS.seek 1ml
@05:37:19.83 delay 1 second
@05:37:20.85 AFV.select :holdingCoil
@05:37:20.96 delay 1 second
@05:37:21.98 ATV.dial :waste
@05:37:23.75 AS.reconfigure ASnorm
@05:37:23.79 AS.seek 0ml
@05:38:14.50 AFV.select :carrierSolution
@05:38:14.61 delay 2 seconds
@05:38:16.63 AS.reconfigure ASconfig
@05:38:16.68 AS.seek 0.5ml
@05:38:45.71 delay 1 second
@05:38:46.74 AFV.select :holdingCoil
@05:38:47.24 Building Slug
@05:38:47.25 ATV.dial :ARV
@05:38:48.97 delay 2 seconds
@05:38:51.00 ARV.dial :air1
@05:38:51.39 delay 2 seconds
@05:38:53.46 AS.reconfigure ASslow2
@05:38:53.51 AS.seek 0.506ml
@05:39:00.28 delay 2 seconds
@05:39:02.31 ARV.dial :MM2
@05:39:03.88 delay 2 seconds
@05:39:05.91 Adding MM2
@05:39:05.97 AS.seek 0.524ml
@05:39:24.31 delay 25 seconds
@05:39:49.34 ARV.dial :PP3
@05:39:49.73 Adding PP3
@05:39:49.74 delay 2 seconds
@05:39:51.80 AS.seek 0.53ml
@05:39:58.33 delay 10 seconds
@05:40:08.37 ARV.dial :air2
@05:40:08.78 adding ARV air
@05:40:08.79 delay 2 seconds
@05:40:10.85 AS.seek 0.536ml
@05:40:17.11 delay 5 seconds
@05:40:22.14 ARV.dial :carrier
@05:40:23.49 delay 7 seconds
@05:40:30.58 AS.seek 0.594ml
@05:41:28.97 delay 5 seconds
@05:41:34.00 ATV.dial :elutionWater
@05:41:35.50 delay 2 seconds
@05:41:37.57 AS.seek 0.6ml
@05:41:43.96 delay 4 seconds
@05:41:47.99 ATV.dial ARV
@05:41:49.47 delay 2 seconds
@05:41:51.50 ARV.dial :carrier
@05:41:51.51 delay 2 seconds
@05:41:53.57 AS.seek 0.65ml
@05:42:44.36 delay 4 seconds
@05:42:48.39 ATV.dial PCR
@05:42:49.17 ASV.dial :waste1
@05:42:49.19 ARV.dial :air1
@05:42:49.59 Positioning Slug by 0.324ml
@05:42:49.66 AS.seek 0.326ml
@05:48:15.75 delay 5 seconds
@05:48:20.78 ATV.dial ARV
@05:48:21.58 ASV.dial :PCRlock
@05:48:21.97 delay 5 seconds
@05:48:27.00 Running AALPP3 Profile58CIQ2 thermocycling
@05:48:27.01 <PCR> PCR.program Profile58CIQ2
@05:48:27.11 Profile58CIQ2 should complete in < 1:36:15
@05:48:27.12 PCR.run 2:05:18.75
@07:09:29.02 PCR.off
@07:09:29.58 <MFBPCR> Running AALPP3 MeltProfile58 thermocycling
@07:09:29.60 <PCR> PCR.program MeltProfile58
@07:09:29.68 MeltProfile58 should complete in < 12:33
@07:09:29.69 PCR.run 20:41.25
@07:17:20.24 PCR.off
@07:17:21.22 <MFBPCR> PCR for AALPP3 completed -- closed /var/log/esp/AALPP321apr0505h.pcr
@07:17:21.28 Cleaning PCR
@07:17:21.29 AFV.select :carrierSolution
@07:17:21.39 delay 2 seconds
@07:17:23.41 AS.reconfigure ASconfig
@07:17:23.46 AS.seek 0.5ml
@07:17:33.78 delay 1 second
@07:17:34.80 AFV.select :holdingCoil
@07:17:34.91 ASV.dial :waste1
@07:17:35.29 ATV.dial :air3
@07:17:36.63 AS.reconfigure ASslow1
@07:17:36.67 AS.seek 0.51ml
@07:17:38.94 delay 2 seconds
@07:17:40.96 ATV.dial :bleach
@07:17:41.58 AS.seek 0.81ml
@07:18:42.14 delay 3 seconds
@07:18:45.17 ATV.dial :air3
@07:18:45.74 delay 3 seconds
@07:18:48.81 AS.seek 0.835ml
@07:18:54.08 delay 3 seconds
@07:18:57.11 ATV.dial :bleach
@07:18:57.74 AS.seek 0.82ml
@07:19:01.05 delay 3 seconds
@07:19:04.08 ARV.dial :waste
@07:19:04.71 delay 3 seconds
@07:19:07.74 ATV.dial :ARV
@07:19:08.64 AS.reconfigure ASconfig
@07:19:08.68 AS.seek 0.47ml
@07:19:29.07 AFV.select :carrierSolution
@07:19:29.18 delay 2 seconds
@07:19:31.20 AS.seek 1ml
@07:20:01.93 delay 1 second
@07:20:02.95 AFV.select :holdingCoil
@07:20:03.47 delay 1 second
@07:20:04.49 AS.reconfigure ASfast
@07:20:04.54 AS.seek 0ml
@07:20:31.86 AFV.select :carrierSolution
@07:20:31.97 delay 2 seconds
@07:20:34.00 AS.reconfigure ASconfig
@07:20:34.05 AS.seek 1ml
@07:21:31.80 delay 1 second
@07:21:32.82 AFV.select :holdingCoil
@07:21:32.93 delay 1 second
@07:21:33.95 AS.reconfigure ASfast
@07:21:34.00 AS.seek 0ml
@07:22:01.32 AFV.select :carrierSolution
@07:22:01.43 delay 2 seconds
@07:22:03.45 AS.reconfigure ASconfig
@07:22:03.50 AS.seek 1ml
@07:23:01.24 delay 1 second
@07:23:02.26 AFV.select :holdingCoil
@07:23:02.37 delay 1 second
@07:23:03.39 AS.reconfigure ASfast
@07:23:03.44 AS.seek 0ml
@07:23:30.79 ARV.dial between carrier and air1
@07:23:31.31 ATV.dial :air3
@07:23:32.64 AS.reconfigure ASslow1
@07:23:32.68 AS.seek 0.01ml
@07:23:35.08 delay 2 seconds
@07:23:37.11 ATV.dial :bleach
@07:23:37.74 AS.seek 0.81ml
@07:26:18.89 delay 3 seconds
@07:26:21.92 ATV.dial :air3
@07:26:22.49 delay 3 seconds
@07:26:25.56 AS.seek 0.835ml
@07:26:30.94 delay 3 seconds
@07:26:33.97 ATV.dial :bleach
@07:26:34.60 AS.seek 0.82ml
@07:26:37.91 delay 3 seconds
@07:26:40.94 ATV.dial PCR
@07:26:41.34 AS.reconfigure ASconfig
@07:26:41.38 AS.seek 0ml
@07:27:28.77 PCR.program :bleachProfile
@07:27:28.83 PCR program should complete in < 2:45
@07:27:28.84 PCR.run 10:00
@07:30:24.61 PCR.off
@07:30:24.63 AFV.select :carrierSolution
@07:30:24.74 delay 2 seconds
@07:30:26.76 AS.seek 1ml
@07:31:24.54 delay 1 second
@07:31:25.56 AFV.select :holdingCoil
@07:31:25.67 delay 1 second
@07:31:26.69 AS.reconfigure ASfast
@07:31:26.74 AS.seek 0ml
@07:31:54.05 delay 3 seconds
@07:31:57.07 AFV.select :carrierSolution
@07:31:57.18 delay 2 seconds
@07:31:59.20 AS.reconfigure ASconfig
@07:31:59.25 AS.seek 1ml
@07:32:56.99 delay 1 second
@07:32:58.01 AFV.select :holdingCoil
@07:32:58.12 delay 1 second
@07:32:59.14 AS.reconfigure ASfast
@07:32:59.19 AS.seek 0ml
@07:33:26.49 delay 3 seconds
@07:33:29.51 AFV.select :carrierSolution
@07:33:29.62 delay 2 seconds
@07:33:31.64 AS.reconfigure ASconfig
@07:33:32.10 AS.seek 1ml
@07:34:29.85 delay 1 second
@07:34:30.87 AFV.select :holdingCoil
@07:34:30.98 delay 1 second
@07:34:32.00 AS.reconfigure ASfast
@07:34:32.05 AS.seek 0ml
@07:34:59.38 delay 3 seconds
@07:35:02.40 AFV.select :carrierSolution
@07:35:02.51 delay 2 seconds
@07:35:04.53 AS.reconfigure ASconfig
@07:35:04.58 AS.seek 1ml
@07:36:02.32 delay 1 second
@07:36:03.34 AFV.select :holdingCoil
@07:36:03.45 delay 1 second
@07:36:04.47 AS.reconfigure ASfast
@07:36:04.52 AS.seek 0ml
@07:36:31.86 delay 3 seconds
@07:36:34.88 AFV.select :carrierSolution
@07:36:34.99 delay 2 seconds
@07:36:37.01 AS.reconfigure ASconfig
@07:36:37.07 AS.seek 1ml
@07:37:34.80 delay 1 second
@07:37:35.82 AFV.select :holdingCoil
@07:37:35.93 delay 1 second
@07:37:36.95 AS.reconfigure ASnorm
@07:37:37.00 AS.seek 0ml
@07:38:27.69 Setting up PCR for ACOPP5 -- writing /var/log/esp/ACOPP521apr0507h.pcr
@07:38:27.70 Building PCR Slug from elutionWater with MM3 mix, PP5 primer
@07:38:27.71 Priming with carrier
@07:38:27.72 ATV.dial ARV
@07:38:28.36 ARV.dial :carrier
@07:38:28.70 AS.seek 0.5ml
@07:38:54.21 AFV.select :carrierSolution
@07:38:54.32 delay 2 seconds
@07:38:56.34 AS.reconfigure ASconfig
@07:38:56.39 AS.seek 1ml
@07:39:25.39 delay 1 second
@07:39:26.41 AFV.select :holdingCoil
@07:39:26.52 delay 1 second
@07:39:27.54 ATV.dial :waste
@07:39:29.31 AS.reconfigure ASnorm
@07:39:29.35 AS.seek 0ml
@07:40:20.07 AFV.select :carrierSolution
@07:40:20.18 delay 2 seconds
@07:40:22.20 AS.reconfigure ASconfig
@07:40:22.25 AS.seek 0.5ml
@07:40:51.27 delay 1 second
@07:40:52.29 AFV.select :holdingCoil
@07:40:52.40 Building Slug
@07:40:52.41 ATV.dial :ARV
@07:40:54.14 delay 2 seconds
@07:40:56.17 ARV.dial :air1
@07:40:56.56 delay 2 seconds
@07:40:58.63 AS.reconfigure ASslow2
@07:40:58.68 AS.seek 0.506ml
@07:41:05.14 delay 2 seconds
@07:41:07.17 ARV.dial :MM3
@07:41:08.25 delay 2 seconds
@07:41:10.28 Adding MM3
@07:41:10.34 AS.seek 0.524ml
@07:41:29.05 delay 25 seconds
@07:41:54.08 ARV.dial :PP5
@07:41:54.48 Adding PP5
@07:41:54.49 delay 2 seconds
@07:41:56.55 AS.seek 0.53ml
@07:42:03.00 delay 10 seconds
@07:42:13.04 ARV.dial :air3
@07:42:13.44 adding ARV air
@07:42:13.46 delay 2 seconds
@07:42:15.52 AS.seek 0.536ml
@07:42:21.80 delay 5 seconds
@07:42:26.83 ARV.dial :carrier
@07:42:28.16 delay 7 seconds
@07:42:35.24 AS.seek 0.594ml
@07:43:33.99 delay 5 seconds
@07:43:39.02 ATV.dial :elutionWater
@07:43:40.52 delay 2 seconds
@07:43:42.59 AS.seek 0.6ml
@07:43:48.94 delay 4 seconds
@07:43:52.97 ATV.dial ARV
@07:43:54.44 delay 2 seconds
@07:43:56.47 ARV.dial :carrier
@07:43:56.48 delay 2 seconds
@07:43:58.54 AS.seek 0.65ml
@07:44:49.25 delay 4 seconds
@07:44:53.28 ATV.dial PCR
@07:44:54.07 ASV.dial :waste1
@07:44:54.09 ARV.dial :air1
@07:44:54.51 Positioning Slug by 0.324ml
@07:44:54.58 AS.seek 0.326ml
@07:50:20.61 delay 5 seconds
@07:50:25.64 ATV.dial ARV
@07:50:26.44 ASV.dial :PCRlock
@07:50:26.83 delay 5 seconds
@07:50:31.86 Running ACOPP5 ProfileIPc thermocycling
@07:50:31.87 <PCR> PCR.program ProfileIPc
@07:50:31.97 ProfileIPc should complete in < 1:26:35
@07:50:31.98 PCR.run 1:53:13.75
@08:59:34.76 PCR.off
@08:59:35.32 <MFBPCR> PCR for ACOPP5 completed -- closed /var/log/esp/ACOPP521apr0507h.pcr
@08:59:35.38 Cleaning PCR
@08:59:35.39 AFV.select :carrierSolution
@08:59:35.49 delay 2 seconds
@08:59:37.51 AS.reconfigure ASconfig
@08:59:37.56 AS.seek 0.5ml
@08:59:47.85 delay 1 second
@08:59:48.87 AFV.select :holdingCoil
@08:59:48.98 ASV.dial :waste1
@08:59:49.36 ATV.dial :air3
@08:59:50.71 AS.reconfigure ASslow1
@08:59:50.75 AS.seek 0.51ml
@08:59:53.00 delay 2 seconds
@08:59:55.02 ATV.dial :bleach
@08:59:55.64 AS.seek 0.81ml
@09:00:56.21 delay 3 seconds
@09:00:59.24 ATV.dial :air3
@09:00:59.80 delay 3 seconds
@09:01:03.27 AS.seek 0.835ml
@09:01:08.55 delay 3 seconds
@09:01:11.58 ATV.dial :bleach
@09:01:12.21 AS.seek 0.82ml
@09:01:15.52 delay 3 seconds
@09:01:18.55 ARV.dial :waste
@09:01:19.18 delay 3 seconds
@09:01:22.21 ATV.dial :ARV
@09:01:23.11 AS.reconfigure ASconfig
@09:01:23.15 AS.seek 0.47ml
@09:01:43.54 AFV.select :carrierSolution
@09:01:43.65 delay 2 seconds
@09:01:45.67 AS.seek 1ml
@09:02:16.40 delay 1 second
@09:02:17.42 AFV.select :holdingCoil
@09:02:17.53 delay 1 second
@09:02:18.55 AS.reconfigure ASfast
@09:02:18.60 AS.seek 0ml
@09:02:45.94 AFV.select :carrierSolution
@09:02:46.05 delay 2 seconds
@09:02:48.07 AS.reconfigure ASconfig
@09:02:48.12 AS.seek 1ml
@09:03:45.86 delay 1 second
@09:03:46.88 AFV.select :holdingCoil
@09:03:46.99 delay 1 second
@09:03:48.01 AS.reconfigure ASfast
@09:03:48.06 AS.seek 0ml
@09:04:15.40 AFV.select :carrierSolution
@09:04:15.51 delay 2 seconds
@09:04:17.53 AS.reconfigure ASconfig
@09:04:17.58 AS.seek 1ml
@09:05:15.30 delay 1 second
@09:05:16.32 AFV.select :holdingCoil
@09:05:16.43 delay 1 second
@09:05:17.45 AS.reconfigure ASfast
@09:05:17.50 AS.seek 0ml
@09:05:44.82 ARV.dial between carrier and air1
@09:05:45.33 ATV.dial :air3
@09:05:46.67 AS.reconfigure ASslow1
@09:05:46.71 AS.seek 0.01ml
@09:05:49.02 delay 2 seconds
@09:05:51.05 ATV.dial :bleach
@09:05:51.67 AS.seek 0.81ml
@09:08:32.79 delay 3 seconds
@09:08:35.82 ATV.dial :air3
@09:08:36.38 delay 3 seconds
@09:08:39.45 AS.seek 0.835ml
@09:08:44.81 delay 3 seconds
@09:08:47.84 ATV.dial :bleach
@09:08:48.47 AS.seek 0.82ml
@09:08:51.74 delay 3 seconds
@09:08:54.77 ATV.dial PCR
@09:08:55.17 AS.reconfigure ASconfig
@09:08:55.21 AS.seek 0ml
@09:09:42.61 PCR.program :bleachProfile
@09:09:42.68 PCR program should complete in < 2:45
@09:09:42.69 PCR.run 10:00
@09:12:38.46 PCR.off
@09:12:38.49 AFV.select :carrierSolution
@09:12:38.60 delay 2 seconds
@09:12:41.03 AS.seek 1ml
@09:13:38.77 delay 1 second
@09:13:39.79 AFV.select :holdingCoil
@09:13:39.90 delay 1 second
@09:13:40.92 AS.reconfigure ASfast
@09:13:40.97 AS.seek 0ml
@09:14:08.30 delay 3 seconds
@09:14:11.32 AFV.select :carrierSolution
@09:14:11.43 delay 2 seconds
@09:14:13.45 AS.reconfigure ASconfig
@09:14:13.50 AS.seek 1ml
@09:15:11.24 delay 1 second
@09:15:12.26 AFV.select :holdingCoil
@09:15:12.37 delay 1 second
@09:15:13.39 AS.reconfigure ASfast
@09:15:13.44 AS.seek 0ml
@09:15:40.75 delay 3 seconds
@09:15:43.77 AFV.select :carrierSolution
@09:15:43.88 delay 2 seconds
@09:15:45.90 AS.reconfigure ASconfig
@09:15:45.96 AS.seek 1ml
@09:16:43.69 delay 1 second
@09:16:44.71 AFV.select :holdingCoil
@09:16:44.82 delay 1 second
@09:16:45.84 AS.reconfigure ASfast
@09:16:45.89 AS.seek 0ml
@09:17:13.22 delay 3 seconds
@09:17:16.24 AFV.select :carrierSolution
@09:17:16.35 delay 2 seconds
@09:17:18.37 AS.reconfigure ASconfig
@09:17:18.42 AS.seek 1ml
@09:18:16.16 delay 1 second
@09:18:17.18 AFV.select :holdingCoil
@09:18:17.29 delay 1 second
@09:18:18.31 AS.reconfigure ASfast
@09:18:18.36 AS.seek 0ml
@09:18:45.68 delay 3 seconds
@09:18:48.70 AFV.select :carrierSolution
@09:18:48.81 delay 2 seconds
@09:18:50.83 AS.reconfigure ASconfig
@09:18:50.89 AS.seek 1ml
@09:19:48.65 delay 1 second
@09:19:49.67 AFV.select :holdingCoil
@09:19:49.78 delay 1 second
@09:19:50.80 AS.reconfigure ASnorm
@09:19:50.85 AS.seek 0ml
@09:20:41.55 Setting up PCR for AIPc -- writing /var/log/esp/AIPc21apr0509h.pcr
@09:20:41.56 Building PCR Slug from elutionWater with MM3 mix, PP6 primer
@09:20:41.58 Priming with carrier
ATV.dial ARV
@09:20:42.22 ARV.dial :carrier
@09:20:42.57 AS.seek 0.5ml
@09:21:08.17 AFV.select :carrierSolution
@09:21:08.28 delay 2 seconds
@09:21:10.30 AS.reconfigure ASconfig
@09:21:10.35 AS.seek 1ml
@09:21:39.38 delay 1 second
@09:21:40.41 AFV.select :holdingCoil
@09:21:40.51 delay 1 second
@09:21:41.53 ATV.dial :waste
@09:21:43.30 AS.reconfigure ASnorm
@09:21:43.34 AS.seek 0ml
@09:22:34.07 AFV.select :carrierSolution
@09:22:34.18 delay 2 seconds
@09:22:36.20 AS.reconfigure ASconfig
@09:22:36.25 AS.seek 0.5ml
@09:23:05.25 delay 1 second
@09:23:06.27 AFV.select :holdingCoil
@09:23:06.38 Building Slug
@09:23:06.39 ATV.dial :ARV
@09:23:08.11 delay 2 seconds
@09:23:10.14 ARV.dial :air1
@09:23:10.53 delay 2 seconds
@09:23:12.60 AS.reconfigure ASslow2
@09:23:12.65 AS.seek 0.506ml
@09:23:19.36 delay 2 seconds
@09:23:21.39 ARV.dial :MM3
@09:23:22.47 delay 2 seconds
@09:23:24.50 Adding MM3
@09:23:24.56 AS.seek 0.524ml
@09:23:43.16 delay 25 seconds
@09:24:08.19 ARV.dial :PP6
@09:24:08.57 Adding PP6
@09:24:08.59 delay 2 seconds
@09:24:10.65 AS.seek 0.53ml
@09:24:16.82 delay 10 seconds
@09:24:26.86 ARV.dial :air1
@09:24:27.71 adding ARV air
@09:24:27.73 delay 2 seconds
@09:24:29.79 AS.seek 0.536ml
@09:24:36.05 delay 5 seconds
@09:24:41.08 ARV.dial :carrier
@09:24:41.49 delay 7 seconds
@09:24:48.56 AS.seek 0.594ml
@09:25:47.44 delay 5 seconds
@09:25:52.47 ATV.dial :elutionWater
@09:25:54.11 delay 2 seconds
@09:25:56.18 AS.seek 0.6ml
@09:26:03.05 delay 4 seconds
@09:26:07.08 ATV.dial ARV
@09:26:08.57 delay 2 seconds
@09:26:10.59 ARV.dial :carrier
@09:26:10.60 delay 2 seconds
@09:26:12.66 AS.seek 0.65ml
@09:27:03.36 delay 4 seconds
@09:27:07.39 ATV.dial PCR
@09:27:08.18 ASV.dial :waste1
@09:27:08.20 ARV.dial :air1
@09:27:08.60 Positioning Slug by 0.324ml
@09:27:08.67 AS.seek 0.326ml
@09:32:34.60 delay 5 seconds
@09:32:39.63 ATV.dial ARV
@09:32:40.41 ASV.dial :PCRlock
@09:32:40.80 delay 5 seconds
@09:32:45.83 Running AIPc ProfileIPc thermocycling
@09:32:45.84 <PCR> PCR.program ProfileIPc
@09:32:45.94 ProfileIPc should complete in < 1:26:35
@09:32:45.95 PCR.run 1:53:13.75
@10:41:55.06 PCR.off
@10:41:55.62 <MFBPCR> PCR for AIPc completed -- closed /var/log/esp/AIPc21apr0509h.pcr
@10:41:55.68 Cleaning PCR
@10:41:56.06 AFV.select :carrierSolution
@10:41:56.18 delay 2 seconds
@10:41:58.20 AS.reconfigure ASconfig
@10:41:58.25 AS.seek 0.5ml
@10:42:08.54 delay 1 second
@10:42:09.56 AFV.select :holdingCoil
@10:42:09.67 ASV.dial :waste1
@10:42:10.05 ATV.dial :air3
@10:42:11.39 AS.reconfigure ASslow1
@10:42:11.43 AS.seek 0.51ml
@10:42:13.70 delay 2 seconds
@10:42:15.73 ATV.dial :bleach
@10:42:16.36 AS.seek 0.81ml
@10:43:16.95 delay 3 seconds
@10:43:20.04 ATV.dial :air3
@10:43:20.61 delay 3 seconds
@10:43:23.68 AS.seek 0.835ml
@10:43:28.96 delay 3 seconds
@10:43:31.99 ATV.dial :bleach
@10:43:32.68 AS.seek 0.82ml
@10:43:36.00 delay 3 seconds
@10:43:39.03 ARV.dial :waste
@10:43:39.65 delay 3 seconds
@10:43:42.68 ATV.dial :ARV
@10:43:43.58 AS.reconfigure ASconfig
@10:43:43.62 AS.seek 0.47ml
@10:44:04.02 AFV.select :carrierSolution
@10:44:04.13 delay 2 seconds
@10:44:06.15 AS.seek 1ml
@10:44:36.88 delay 1 second
@10:44:37.90 AFV.select :holdingCoil
@10:44:38.01 delay 1 second
@10:44:39.03 AS.reconfigure ASfast
@10:44:39.08 AS.seek 0ml
@10:45:06.38 AFV.select :carrierSolution
@10:45:06.49 delay 2 seconds
@10:45:08.51 AS.reconfigure ASconfig
@10:45:08.56 AS.seek 1ml
@10:46:06.29 delay 1 second
@10:46:07.31 AFV.select :holdingCoil
@10:46:07.42 delay 1 second
@10:46:08.44 AS.reconfigure ASfast
@10:46:08.49 AS.seek 0ml
@10:46:35.79 AFV.select :carrierSolution
@10:46:35.90 delay 2 seconds
@10:46:37.92 AS.reconfigure ASconfig
@10:46:37.97 AS.seek 1ml
@10:47:35.71 delay 1 second
@10:47:36.73 AFV.select :holdingCoil
@10:47:36.84 delay 1 second
@10:47:37.86 AS.reconfigure ASfast
@10:47:37.91 AS.seek 0ml
@10:48:05.24 ARV.dial between carrier and air1
@10:48:05.75 ATV.dial :air3
@10:48:07.08 AS.reconfigure ASslow1
@10:48:07.12 AS.seek 0.01ml
@10:48:09.52 delay 2 seconds
@10:48:11.55 ATV.dial :bleach
@10:48:12.16 AS.seek 0.81ml
@10:50:53.43 delay 3 seconds
@10:50:56.46 ATV.dial :air3
@10:50:57.02 delay 3 seconds
@10:51:00.09 AS.seek 0.835ml
@10:51:05.37 delay 3 seconds
@10:51:08.40 ATV.dial :bleach
@10:51:09.03 AS.seek 0.82ml
@10:51:12.32 delay 3 seconds
@10:51:15.35 ATV.dial PCR
@10:51:15.75 AS.reconfigure ASconfig
@10:51:15.79 AS.seek 0ml
@10:52:03.21 PCR.program :bleachProfile
@10:52:03.28 PCR program should complete in < 2:45
@10:52:03.29 PCR.run 10:00
@10:54:59.50 PCR.off
@10:54:59.52 AFV.select :carrierSolution
@10:54:59.63 delay 2 seconds
@10:55:01.65 AS.seek 1ml
@10:55:59.43 delay 1 second
@10:56:00.45 AFV.select :holdingCoil
@10:56:00.56 delay 1 second
@10:56:01.58 AS.reconfigure ASfast
@10:56:01.63 AS.seek 0ml
@10:56:28.94 delay 3 seconds
@10:56:31.96 AFV.select :carrierSolution
@10:56:32.07 delay 2 seconds
@10:56:34.09 AS.reconfigure ASconfig
@10:56:34.14 AS.seek 1ml
@10:57:31.86 delay 1 second
@10:57:32.88 AFV.select :holdingCoil
@10:57:32.99 delay 1 second
@10:57:34.01 AS.reconfigure ASfast
@10:57:34.06 AS.seek 0ml
@10:58:01.40 delay 3 seconds
@10:58:04.42 AFV.select :carrierSolution
@10:58:04.53 delay 2 seconds
@10:58:06.55 AS.reconfigure ASconfig
@10:58:06.61 AS.seek 1ml
@10:59:04.38 delay 1 second
@10:59:05.40 AFV.select :holdingCoil
@10:59:05.51 delay 1 second
@10:59:06.53 AS.reconfigure ASfast
@10:59:06.58 AS.seek 0ml
@10:59:33.91 delay 3 seconds
@10:59:36.93 AFV.select :carrierSolution
@10:59:37.04 delay 2 seconds
@10:59:39.06 AS.reconfigure ASconfig
@10:59:39.11 AS.seek 1ml
@11:00:36.85 delay 1 second
@11:00:37.87 AFV.select :holdingCoil
@11:00:37.99 delay 1 second
@11:00:39.01 AS.reconfigure ASfast
@11:00:39.06 AS.seek 0ml
@11:01:06.38 delay 3 seconds
@11:01:09.40 AFV.select :carrierSolution
@11:01:09.51 delay 2 seconds
@11:01:11.53 AS.reconfigure ASconfig
@11:01:11.59 AS.seek 1ml
@11:02:09.36 delay 1 second
@11:02:10.38 AFV.select :holdingCoil
@11:02:10.49 delay 1 second
@11:02:11.51 AS.reconfigure ASnorm
@11:02:11.56 AS.seek 0ml
@21:35:00.16CEST05-Apr-21 <MAIN> ESP2 v3.30 I2C Gateway v3.06 for core bus
@21:35:02.70 
Configuration for mer 7/20/20 brent@mbari.org
/home/esp/esp2/type/shallow/mer/configure.rb
@21:35:02.79 
Configuration for MFB3 by preston@mbari.org
/home/esp/esp2/type/mfb/MFB3/configure.rb
@21:35:07.51 Polling Can every 10:00
@21:35:09.23 CS.configure :default
@21:35:09.26 CS.hold
@21:35:09.32 CS.configure :default
@21:35:09.40 PS.configure :default
@21:35:09.48 SS.configure :default
@21:35:09.52 SS.hold
@21:35:09.57 SS.configure :default
@21:35:09.65 CC.configure :default
@21:35:09.70 CC.hold
@21:35:09.76 CC.configure :default
@21:35:09.84 PC.configure :default
@21:35:09.88 PC.hold
@21:35:09.94 PC.configure :default
@21:35:10.04 Forearm.configure :loose
@21:35:10.08 Forearm.hold
@21:35:10.14 Forearm.configure :loose
@21:35:10.22 Elbow.configure :loose
@21:35:10.26 Elbow.hold
@21:35:10.30 Elbow.configure :loose
@21:35:10.40 SC.configure :loose
@21:35:10.44 SC.hold
@21:35:10.50 SC.configure :loose
@21:35:10.58 SE.configure :default
@21:35:10.62 SE.hold
@21:35:10.67 SE.configure :default
@21:35:10.71 CH.coast
@21:35:10.75 CH.configure :default
@21:35:10.78 PH.coast
@21:35:10.83 PH.configure :default
@21:35:10.93 SPE.coast
@21:35:10.98 SPE.configure SPEconfig
@21:35:11.14 AS.configure ASconfig
@21:35:11.20 AS.hold
@21:35:11.30 AS.configure ASconfig
@21:35:11.39 PCR.off
@21:35:11.44 PCR.pidtemp= 50,0.1,0,300,25,25
@21:35:11.49 PCR.caltemp= 2.5051,0.0372,0
@21:35:11.54 All dwarf controllers running firmware version 3.75
@21:35:11.81 Cleanly exited at 11:03:02CEST05-Apr-21
@21:35:11.84 Loading /home/esp/esp2/mission/GENOMAP_v2.rb mission
@21:35:11.90 Cleaning PCR
AFV.select :carrierSolution
@21:35:12.00 delay 2 seconds
@21:35:14.02 AS.seek 0.5ml
@21:35:14.72 <mwj> Session Begun
@21:35:29.63 -> showlog n 10
@21:35:29.65 NoMethodError in mwj -- undefined method `n' for Object:Class
@21:35:35.78 -> showlog -n 10
@21:35:35.79 SyntaxError in mwj -- compile error
mwj:2: syntax error, unexpected tINTEGER, expecting kDO or '{' or '('
showlog -n 10
             ^
@21:35:43.05 <MAIN> delay 1 second
@21:35:44.07 AFV.select :holdingCoil
@21:35:44.18 ASV.dial :waste1
@21:35:44.27 ATV.dial :air3
@21:35:45.16 AS.reconfigure ASslow1
@21:35:45.22 AS.seek 0.51ml
@21:35:47.62 delay 2 seconds
@21:35:49.65 ATV.dial :bleach
@21:35:50.30 AS.seek 0.81ml
@21:35:57.75 <mwj> -> exit
@21:35:57.76 Session Ended
@21:36:50.90 <MAIN> delay 3 seconds
@21:36:53.93 ATV.dial :air3
@21:36:54.49 delay 3 seconds
@21:36:57.56 AS.seek 0.835ml
@21:37:02.84 delay 3 seconds
@21:37:05.87 ATV.dial :bleach
@21:37:06.49 AS.seek 0.82ml
@21:37:09.91 delay 3 seconds
@21:37:12.94 ARV.dial :waste
@21:37:13.44 delay 3 seconds
@21:37:16.47 ATV.dial :ARV
@21:37:17.39 AS.reconfigure ASconfig
@21:37:17.43 AS.seek 0.47ml
@21:37:37.83 AFV.select :carrierSolution
@21:37:37.94 delay 2 seconds
@21:37:39.96 AS.seek 1ml
@21:38:10.71 delay 1 second
@21:38:11.73 AFV.select :holdingCoil
@21:38:11.84 delay 1 second
@21:38:12.86 AS.reconfigure ASfast
@21:38:12.91 AS.seek 0ml
@21:38:40.24 AFV.select :carrierSolution
@21:38:40.35 delay 2 seconds
@21:38:42.37 AS.reconfigure ASconfig
@21:38:42.42 AS.seek 1ml
@21:39:40.16 delay 1 second
@21:39:41.17 AFV.select :holdingCoil
@21:39:41.28 delay 1 second
@21:39:42.30 AS.reconfigure ASfast
@21:39:42.35 AS.seek 0ml
@21:40:09.68 AFV.select :carrierSolution
@21:40:09.79 delay 2 seconds
@21:40:11.81 AS.reconfigure ASconfig
@21:40:12.23 AS.seek 1ml
@21:41:09.98 delay 1 second
@21:41:10.99 AFV.select :holdingCoil
@21:41:11.10 delay 1 second
@21:41:12.12 AS.reconfigure ASfast
@21:41:12.17 AS.seek 0ml
@21:41:39.49 ARV.dial between carrier and air1
@21:41:40.02 ATV.dial :air3
@21:41:41.35 AS.reconfigure ASslow1
@21:41:41.39 AS.seek 0.01ml
@21:41:43.79 delay 2 seconds
@21:41:45.82 ATV.dial :bleach
@21:41:46.44 AS.seek 0.81ml
@21:44:27.57 delay 3 seconds
@21:44:30.60 ATV.dial :air3
@21:44:31.16 delay 3 seconds
@21:44:34.23 AS.seek 0.835ml
@21:44:39.50 delay 3 seconds
@21:44:42.53 ATV.dial :bleach
@21:44:43.16 AS.seek 0.82ml
@21:44:46.51 delay 3 seconds
@21:44:49.54 ATV.dial PCR
@21:44:49.95 AS.reconfigure ASconfig
@21:44:49.99 AS.seek 0ml
@21:45:37.40 PCR.program :bleachProfile
@21:45:37.46 PCR program should complete in < 2:45
@21:45:37.47 PCR.run 10:00
@21:48:39.36 PCR.off
@21:48:39.39 AFV.select :carrierSolution
@21:48:39.49 delay 2 seconds
@21:48:41.51 AS.seek 1ml
@21:49:39.26 delay 1 second
@21:49:40.28 AFV.select :holdingCoil
@21:49:40.39 delay 1 second
@21:49:41.41 AS.reconfigure ASfast
@21:49:41.46 AS.seek 0ml
@21:50:08.77 delay 3 seconds
@21:50:11.79 AFV.select :carrierSolution
@21:50:11.90 delay 2 seconds
@21:50:13.92 AS.reconfigure ASconfig
@21:50:13.97 AS.seek 1ml
@21:51:11.74 delay 1 second
@21:51:12.75 AFV.select :holdingCoil
@21:51:12.86 delay 1 second
@21:51:13.88 AS.reconfigure ASfast
@21:51:13.93 AS.seek 0ml
@21:51:41.24 delay 3 seconds
@21:51:44.26 AFV.select :carrierSolution
@21:51:44.37 delay 2 seconds
@21:51:46.39 AS.reconfigure ASconfig
@21:51:46.63 AS.seek 1ml
@21:52:44.37 delay 1 second
@21:52:45.39 AFV.select :holdingCoil
@21:52:45.50 delay 1 second
@21:52:46.52 AS.reconfigure ASfast
@21:52:46.57 AS.seek 0ml
@21:53:13.90 delay 3 seconds
@21:53:16.92 AFV.select :carrierSolution
@21:53:17.03 delay 2 seconds
@21:53:19.05 AS.reconfigure ASconfig
@21:53:19.10 AS.seek 1ml
@21:54:16.83 delay 1 second
@21:54:17.84 AFV.select :holdingCoil
@21:54:17.94 delay 1 second
@21:54:18.96 AS.reconfigure ASfast
@21:54:19.01 AS.seek 0ml
@21:54:46.33 delay 3 seconds
@21:54:49.35 AFV.select :carrierSolution
@21:54:49.46 delay 2 seconds
@21:54:51.48 AS.reconfigure ASconfig
@21:54:51.53 AS.seek 1ml
@21:55:49.29 delay 1 second
@21:55:50.30 AFV.select :holdingCoil
@21:55:50.41 delay 1 second
@21:55:51.43 AS.reconfigure ASnorm
@21:55:51.48 AS.seek 0ml
@21:56:42.26 Cleaning PCR
@21:56:42.27 AFV.select :carrierSolution
@21:56:42.37 delay 2 seconds
@21:56:44.39 AS.reconfigure ASconfig
@21:56:44.44 AS.seek 0.5ml
@21:57:13.46 delay 1 second
@21:57:14.47 AFV.select :holdingCoil
@21:57:14.58 ASV.dial :waste1
@21:57:14.59 ATV.dial :air3
@21:57:15.46 AS.reconfigure ASslow1
@21:57:15.50 AS.seek 0.51ml
@21:57:17.79 delay 2 seconds
@21:57:19.81 ATV.dial :bleach
@21:57:20.44 AS.seek 0.81ml
@21:58:20.99 delay 3 seconds
@21:58:24.02 ATV.dial :air3
@21:58:24.59 delay 3 seconds
@21:58:27.66 AS.seek 0.835ml
@21:58:33.07 delay 3 seconds
@21:58:36.10 ATV.dial :bleach
@21:58:36.71 AS.seek 0.82ml
@21:58:39.99 delay 3 seconds
@21:58:43.02 ARV.dial :waste
@21:58:43.52 delay 3 seconds
@21:58:46.55 ATV.dial :ARV
@21:58:47.79 AS.reconfigure ASconfig
@21:58:47.87 AS.seek 0.47ml
@21:59:08.26 AFV.select :carrierSolution
@21:59:08.37 delay 2 seconds
@21:59:10.39 AS.seek 1ml
@21:59:41.16 delay 1 second
@21:59:42.18 AFV.select :holdingCoil
@21:59:42.29 delay 1 second
@21:59:43.31 AS.reconfigure ASfast
@21:59:43.36 AS.seek 0ml
@22:00:10.68 AFV.select :carrierSolution
@22:00:10.79 delay 2 seconds
@22:00:12.81 AS.reconfigure ASconfig
@22:00:12.86 AS.seek 1ml
@22:01:10.60 delay 1 second
@22:01:11.62 AFV.select :holdingCoil
@22:01:11.73 delay 1 second
@22:01:12.75 AS.reconfigure ASfast
@22:01:12.80 AS.seek 0ml
@22:01:40.10 AFV.select :carrierSolution
@22:01:40.21 delay 2 seconds
@22:01:42.23 AS.reconfigure ASconfig
@22:01:42.28 AS.seek 1ml
@22:02:40.02 delay 1 second
@22:02:41.04 AFV.select :holdingCoil
@22:02:41.15 delay 1 second
@22:02:42.17 AS.reconfigure ASfast
@22:02:42.22 AS.seek 0ml
@22:03:09.54 ARV.dial between carrier and air1
@22:03:10.06 ATV.dial :air3
@22:03:11.40 AS.reconfigure ASslow1
@22:03:11.44 AS.seek 0.01ml
@22:03:13.85 delay 2 seconds
@22:03:15.88 ATV.dial :bleach
@22:03:16.50 AS.seek 0.81ml
@22:05:57.69 delay 3 seconds
@22:06:00.72 ATV.dial :air3
@22:06:01.29 delay 3 seconds
@22:06:04.36 AS.seek 0.835ml
@22:06:09.70 delay 3 seconds
@22:06:12.73 ATV.dial :bleach
@22:06:13.35 AS.seek 0.82ml
@22:06:16.73 delay 3 seconds
@22:06:19.76 ATV.dial PCR
@22:06:20.17 AS.reconfigure ASconfig
@22:06:20.21 AS.seek 0ml
@22:07:07.60 PCR.program :bleachProfile
@22:07:07.66 PCR program should complete in < 2:45
@22:07:07.67 PCR.run 10:00
@22:10:09.50 PCR.off
@22:10:09.52 AFV.select :carrierSolution
@22:10:09.63 delay 2 seconds
@22:10:11.65 AS.seek 1ml
@22:11:09.40 delay 1 second
@22:11:10.42 AFV.select :holdingCoil
@22:11:10.53 delay 1 second
@22:11:11.55 AS.reconfigure ASfast
@22:11:11.60 AS.seek 0ml
@22:11:38.93 delay 3 seconds
@22:11:41.95 AFV.select :carrierSolution
@22:11:42.06 delay 2 seconds
@22:11:44.08 AS.reconfigure ASconfig
@22:11:44.13 AS.seek 1ml
@22:12:41.87 delay 1 second
@22:12:42.89 AFV.select :holdingCoil
@22:12:43.00 delay 1 second
@22:12:44.02 AS.reconfigure ASfast
@22:12:44.07 AS.seek 0ml
@22:13:11.40 delay 3 seconds
@22:13:14.42 AFV.select :carrierSolution
@22:13:14.53 delay 2 seconds
@22:13:16.55 AS.reconfigure ASconfig
@22:13:16.61 AS.seek 1ml
@22:14:14.35 delay 1 second
@22:14:15.36 AFV.select :holdingCoil
@22:14:15.47 delay 1 second
@22:14:16.49 AS.reconfigure ASfast
@22:14:16.54 AS.seek 0ml
@22:14:43.87 delay 3 seconds
@22:14:46.89 AFV.select :carrierSolution
@22:14:47.00 delay 2 seconds
@22:14:49.02 AS.reconfigure ASconfig
@22:14:49.07 AS.seek 1ml
@22:15:46.82 delay 1 second
@22:15:47.83 AFV.select :holdingCoil
@22:15:47.94 delay 1 second
@22:15:48.96 AS.reconfigure ASfast
@22:15:49.01 AS.seek 0ml
@22:16:16.35 delay 3 seconds
@22:16:19.37 AFV.select :carrierSolution
@22:16:19.48 delay 2 seconds
@22:16:21.50 AS.reconfigure ASconfig
@22:16:21.55 AS.seek 1ml
@22:17:19.32 delay 1 second
@22:17:20.33 AFV.select :holdingCoil
@22:17:20.44 delay 1 second
@22:17:21.46 AS.reconfigure ASnorm
@22:17:21.51 AS.seek 0ml
@22:18:12.27 Cleaning PCR
@22:18:12.28 AFV.select :carrierSolution
@22:18:12.38 delay 2 seconds
@22:18:14.40 AS.reconfigure ASconfig
@22:18:14.45 AS.seek 0.5ml
@22:18:43.48 delay 1 second
@22:18:44.49 AFV.select :holdingCoil
@22:18:44.59 ASV.dial :waste1
@22:18:44.60 ATV.dial :air3
@22:18:45.46 AS.reconfigure ASslow1
@22:18:45.50 AS.seek 0.51ml
@22:18:47.77 delay 2 seconds
@22:18:49.80 ATV.dial :bleach
@22:18:50.79 AS.seek 0.81ml
@22:19:51.38 delay 3 seconds
@22:19:54.41 ATV.dial :air3
@22:19:54.98 delay 3 seconds
@22:19:58.05 AS.seek 0.835ml
@22:20:03.44 delay 3 seconds
@22:20:06.47 ATV.dial :bleach
@22:20:07.08 AS.seek 0.82ml
@22:20:10.37 delay 3 seconds
@22:20:13.40 ARV.dial :waste
@22:20:13.90 delay 3 seconds
@22:20:16.93 ATV.dial :ARV
@22:20:17.85 AS.reconfigure ASconfig
@22:20:17.89 AS.seek 0.47ml
@22:20:38.29 AFV.select :carrierSolution
@22:20:38.40 delay 2 seconds
@22:20:40.42 AS.seek 1ml
@22:21:11.19 delay 1 second
@22:21:12.21 AFV.select :holdingCoil
@22:21:12.32 delay 1 second
@22:21:13.34 AS.reconfigure ASfast
@22:21:13.39 AS.seek 0ml
@22:21:40.71 AFV.select :carrierSolution
@22:21:40.82 delay 2 seconds
@22:21:42.84 AS.reconfigure ASconfig
@22:21:42.89 AS.seek 1ml
@22:22:40.63 delay 1 second
@22:22:41.64 AFV.select :holdingCoil
@22:22:41.75 delay 1 second
@22:22:42.77 AS.reconfigure ASfast
@22:22:42.82 AS.seek 0ml
@22:23:10.15 AFV.select :carrierSolution
@22:23:10.26 delay 2 seconds
@22:23:12.28 AS.reconfigure ASconfig
@22:23:12.33 AS.seek 1ml
@22:24:10.07 delay 1 second
@22:24:11.08 AFV.select :holdingCoil
@22:24:11.19 delay 1 second
@22:24:12.21 AS.reconfigure ASfast
@22:24:12.26 AS.seek 0ml
@22:24:39.59 ARV.dial between carrier and air1
@22:24:40.10 ATV.dial :air3
@22:24:41.44 AS.reconfigure ASslow1
@22:24:41.48 AS.seek 0.01ml
@22:24:43.77 delay 2 seconds
@22:24:45.80 ATV.dial :bleach
@22:24:46.43 AS.seek 0.81ml
@22:27:27.55 delay 3 seconds
@22:27:30.58 ATV.dial :air3
@22:27:31.15 delay 3 seconds
@22:27:34.22 AS.seek 0.835ml
@22:27:39.64 delay 3 seconds
@22:27:42.67 ATV.dial :bleach
@22:27:43.30 AS.seek 0.82ml
@22:27:46.52 delay 3 seconds
@22:27:49.55 ATV.dial PCR
@22:27:49.95 AS.reconfigure ASconfig
@22:27:49.99 AS.seek 0ml
@22:28:37.40 PCR.program :bleachProfile
@22:28:37.46 PCR program should complete in < 2:45
@22:28:37.47 PCR.run 10:00
@22:31:39.31 PCR.off
@22:31:39.33 AFV.select :carrierSolution
@22:31:39.44 delay 2 seconds
@22:31:41.46 AS.seek 1ml
@22:32:39.21 delay 1 second
@22:32:40.23 AFV.select :holdingCoil
@22:32:40.34 delay 1 second
@22:32:41.36 AS.reconfigure ASfast
@22:32:41.41 AS.seek 0ml
@22:33:08.73 delay 3 seconds
@22:33:11.75 AFV.select :carrierSolution
@22:33:11.86 delay 2 seconds
@22:33:13.88 AS.reconfigure ASconfig
@22:33:13.93 AS.seek 1ml
@22:34:11.69 delay 1 second
@22:34:12.70 AFV.select :holdingCoil
@22:34:12.81 delay 1 second
@22:34:13.83 AS.reconfigure ASfast
@22:34:13.91 AS.seek 0ml
@22:34:41.24 delay 3 seconds
@22:34:44.26 AFV.select :carrierSolution
@22:34:44.39 delay 2 seconds
@22:34:46.42 AS.reconfigure ASconfig
@22:34:46.50 AS.seek 1ml
@22:35:44.24 delay 1 second
@22:35:45.25 AFV.select :holdingCoil
@22:35:45.36 delay 1 second
@22:35:46.38 AS.reconfigure ASfast
@22:35:46.43 AS.seek 0ml
@22:36:13.73 delay 3 seconds
@22:36:16.75 AFV.select :carrierSolution
@22:36:16.86 delay 2 seconds
@22:36:18.88 AS.reconfigure ASconfig
@22:36:18.93 AS.seek 1ml
@22:37:16.68 delay 1 second
@22:37:17.69 AFV.select :holdingCoil
@22:37:17.80 delay 1 second
@22:37:18.82 AS.reconfigure ASfast
@22:37:18.87 AS.seek 0ml
@22:37:46.21 delay 3 seconds
@22:37:49.23 AFV.select :carrierSolution
@22:37:49.34 delay 2 seconds
@22:37:51.36 AS.reconfigure ASconfig
@22:37:51.41 AS.seek 1ml
@22:38:49.15 delay 1 second
@22:38:50.17 AFV.select :holdingCoil
@22:38:50.64 delay 1 second
@22:38:51.66 AS.reconfigure ASnorm
@22:38:51.71 AS.seek 0ml
@22:39:42.53 <MFBPCR> Setting up PCR for ACYPP2 -- writing /var/log/esp/ACYPP221apr0522h.pcr
@22:39:42.55 Building PCR Slug from elutionWater with MM1 mix, PP2 primer
@22:39:42.56 Priming with carrier
@22:39:42.57 ATV.dial ARV
@22:39:43.21 ARV.dial :carrier
@22:39:43.55 AS.seek 0.5ml
@22:40:09.12 AFV.select :carrierSolution
@22:40:09.23 delay 2 seconds
@22:40:11.25 AS.reconfigure ASconfig
@22:40:11.30 AS.seek 1ml
@22:40:40.30 delay 1 second
@22:40:41.32 AFV.select :holdingCoil
@22:40:41.43 delay 1 second
@22:40:42.45 ATV.dial :waste
@22:40:44.20 AS.reconfigure ASnorm
@22:40:44.24 AS.seek 0ml
@22:41:34.91 AFV.select :carrierSolution
@22:41:35.02 delay 2 seconds
@22:41:37.04 AS.reconfigure ASconfig
@22:41:37.09 AS.seek 0.5ml
@22:42:06.10 delay 1 second
@22:42:07.12 AFV.select :holdingCoil
@22:42:07.23 Building Slug
@22:42:07.24 ATV.dial :ARV
@22:42:08.95 delay 2 seconds
@22:42:10.98 ARV.dial :air1
@22:42:11.38 delay 2 seconds
@22:42:13.45 AS.reconfigure ASslow2
@22:42:13.50 AS.seek 0.506ml
@22:42:19.91 delay 2 seconds
@22:42:21.94 ARV.dial :MM1
@22:42:22.55 delay 2 seconds
@22:42:24.58 Adding MM1
@22:42:24.64 AS.seek 0.524ml
@22:42:42.99 delay 25 seconds
@22:43:08.02 ARV.dial :PP2
@22:43:08.42 Adding PP2
@22:43:08.43 delay 2 seconds
@22:43:10.49 AS.seek 0.53ml
@22:43:16.99 delay 10 seconds
@22:43:27.03 ARV.dial :air2
@22:43:27.44 adding ARV air
@22:43:27.45 delay 2 seconds
@22:43:29.51 AS.seek 0.536ml
@22:43:35.82 delay 5 seconds
@22:43:40.85 ARV.dial :carrier
@22:43:42.18 delay 7 seconds
@22:43:49.26 AS.seek 0.594ml
@22:44:47.85 delay 5 seconds
@22:44:52.88 ATV.dial :elutionWater
@22:44:54.38 delay 2 seconds
@22:44:56.45 AS.seek 0.6ml
@22:45:02.73 delay 4 seconds
@22:45:06.76 ATV.dial ARV
@22:45:08.23 delay 2 seconds
@22:45:10.26 ARV.dial :carrier
@22:45:10.27 delay 2 seconds
@22:45:12.33 AS.seek 0.65ml
@22:46:02.95 delay 4 seconds
@22:46:06.98 ATV.dial PCR
@22:46:07.77 ASV.dial :waste1
@22:46:07.79 ARV.dial :air1
@22:46:08.19 Positioning Slug by 0.324ml
@22:46:08.26 AS.seek 0.326ml
@22:51:34.46 delay 5 seconds
@22:51:39.49 ATV.dial ARV
@22:51:40.29 ASV.dial :PCRlock
@22:51:40.68 delay 5 seconds
@22:51:45.71 Running ACYPP2 Profile60CIQ2 thermocycling
@22:51:45.72 <PCR> PCR.program Profile60CIQ2
@22:51:45.82 Profile60CIQ2 should complete in < 1:47:50
@22:51:45.83 PCR.run 2:19:47.5
@00:20:21.13CEST06-Apr-21 PCR.off
@00:20:21.75 <MFBPCR> Running ACYPP2 MeltProfile60 thermocycling
@00:20:21.77 <PCR> PCR.program MeltProfile60
@00:20:21.85 MeltProfile60 should complete in < 11:55
@00:20:21.86 PCR.run 19:53.75
@00:27:53.66 PCR.off
@00:27:54.59 <MFBPCR> PCR for ACYPP2 completed -- closed /var/log/esp/ACYPP221apr0522h.pcr
@00:27:54.65 Cleaning PCR
@00:27:54.66 AFV.select :carrierSolution
@00:27:54.77 delay 2 seconds
@00:27:56.79 AS.reconfigure ASconfig
@00:27:56.84 AS.seek 0.5ml
@00:28:07.17 delay 1 second
@00:28:08.19 AFV.select :holdingCoil
@00:28:08.30 ASV.dial :waste1
@00:28:08.68 ATV.dial :air3
@00:28:10.02 AS.reconfigure ASslow1
@00:28:10.06 AS.seek 0.51ml
@00:28:12.35 delay 2 seconds
@00:28:14.37 ATV.dial :bleach
@00:28:14.98 AS.seek 0.81ml
@00:29:16.02 delay 3 seconds
@00:29:19.05 ATV.dial :air3
@00:29:19.60 delay 3 seconds
@00:29:22.67 AS.seek 0.835ml
@00:29:28.00 delay 3 seconds
@00:29:31.03 ATV.dial :bleach
@00:29:31.65 AS.seek 0.82ml
@00:29:34.99 delay 3 seconds
@00:29:38.02 ARV.dial :waste
@00:29:38.65 delay 3 seconds
@00:29:41.68 ATV.dial :ARV
@00:29:42.60 AS.reconfigure ASconfig
@00:29:42.64 AS.seek 0.47ml
@00:30:03.04 AFV.select :carrierSolution
@00:30:03.15 delay 2 seconds
@00:30:05.17 AS.seek 1ml
@00:30:35.90 delay 1 second
@00:30:36.92 AFV.select :holdingCoil
@00:30:37.03 delay 1 second
@00:30:38.05 AS.reconfigure ASfast
@00:30:38.10 AS.seek 0ml
@00:31:05.42 AFV.select :carrierSolution
@00:31:05.53 delay 2 seconds
@00:31:07.55 AS.reconfigure ASconfig
@00:31:07.60 AS.seek 1ml
@00:32:05.37 delay 1 second
@00:32:06.39 AFV.select :holdingCoil
@00:32:06.50 delay 1 second
@00:32:07.52 AS.reconfigure ASfast
@00:32:07.57 AS.seek 0ml
@00:32:34.90 AFV.select :carrierSolution
@00:32:35.01 delay 2 seconds
@00:32:37.03 AS.reconfigure ASconfig
@00:32:37.08 AS.seek 1ml
@00:33:34.82 delay 1 second
@00:33:35.84 AFV.select :holdingCoil
@00:33:35.95 delay 1 second
@00:33:36.97 AS.reconfigure ASfast
@00:33:37.02 AS.seek 0ml
@00:34:04.35 ARV.dial between carrier and air1
@00:34:04.87 ATV.dial :air3
@00:34:06.21 AS.reconfigure ASslow1
@00:34:06.25 AS.seek 0.01ml
@00:34:08.63 delay 2 seconds
@00:34:10.66 ATV.dial :bleach
@00:34:11.29 AS.seek 0.81ml
@00:36:52.45 delay 3 seconds
@00:36:55.48 ATV.dial :air3
@00:36:56.04 delay 3 seconds
@00:36:59.11 AS.seek 0.835ml
@00:37:04.39 delay 3 seconds
@00:37:07.42 ATV.dial :bleach
@00:37:08.05 AS.seek 0.82ml
@00:37:11.34 delay 3 seconds
@00:37:14.37 ATV.dial PCR
@00:37:14.77 AS.reconfigure ASconfig
@00:37:14.81 AS.seek 0ml
@00:38:02.21 PCR.program :bleachProfile
@00:38:02.28 PCR program should complete in < 2:45
@00:38:02.29 PCR.run 10:00
@00:40:58.38 PCR.off
@00:40:58.46 AFV.select :carrierSolution
@00:40:58.57 delay 2 seconds
@00:41:00.59 AS.seek 1ml
@00:41:58.34 delay 1 second
@00:41:59.36 AFV.select :holdingCoil
@00:41:59.47 delay 1 second
@00:42:00.49 AS.reconfigure ASfast
@00:42:00.54 AS.seek 0ml
@00:42:27.85 delay 3 seconds
@00:42:30.87 AFV.select :carrierSolution
@00:42:30.98 delay 2 seconds
@00:42:33.00 AS.reconfigure ASconfig
@00:42:33.06 AS.seek 1ml
@00:43:30.81 delay 1 second
@00:43:31.83 AFV.select :holdingCoil
@00:43:31.94 delay 1 second
@00:43:32.96 AS.reconfigure ASfast
@00:43:33.01 AS.seek 0ml
@00:44:00.34 delay 3 seconds
@00:44:03.36 AFV.select :carrierSolution
@00:44:03.47 delay 2 seconds
@00:44:05.49 AS.reconfigure ASconfig
@00:44:05.54 AS.seek 1ml
@00:45:03.27 delay 1 second
@00:45:04.29 AFV.select :holdingCoil
@00:45:04.40 delay 1 second
@00:45:05.42 AS.reconfigure ASfast
@00:45:05.47 AS.seek 0ml
@00:45:32.79 delay 3 seconds
@00:45:35.81 AFV.select :carrierSolution
@00:45:35.92 delay 2 seconds
@00:45:37.94 AS.reconfigure ASconfig
@00:45:38.00 AS.seek 1ml
@00:46:35.74 delay 1 second
@00:46:36.76 AFV.select :holdingCoil
@00:46:36.87 delay 1 second
@00:46:37.89 AS.reconfigure ASfast
@00:46:37.94 AS.seek 0ml
@00:47:05.27 delay 3 seconds
@00:47:08.29 AFV.select :carrierSolution
@00:47:08.40 delay 2 seconds
@00:47:10.42 AS.reconfigure ASconfig
@00:47:10.47 AS.seek 1ml
@00:48:08.23 delay 1 second
@00:48:09.25 AFV.select :holdingCoil
@00:48:09.36 delay 1 second
@00:48:10.38 AS.reconfigure ASnorm
@00:48:10.43 AS.seek 0ml
@00:49:01.13 Setting up PCR for AOPP1 -- writing /var/log/esp/AOPP121apr0600h.pcr
@00:49:01.14 Building PCR Slug from elutionWater with MM1 mix, PP1 primer
@00:49:01.15 Priming with carrier
@00:49:01.16 ATV.dial ARV
@00:49:01.79 ARV.dial :carrier
@00:49:02.13 AS.seek 0.5ml
@00:49:27.70 AFV.select :carrierSolution
@00:49:27.81 delay 2 seconds
@00:49:29.83 AS.reconfigure ASconfig
@00:49:29.88 AS.seek 1ml
@00:49:58.88 delay 1 second
@00:49:59.90 AFV.select :holdingCoil
@00:50:00.01 delay 1 second
@00:50:01.03 ATV.dial :waste
@00:50:02.78 AS.reconfigure ASnorm
@00:50:02.82 AS.seek 0ml
@00:50:53.51 AFV.select :carrierSolution
@00:50:53.65 delay 2 seconds
@00:50:55.68 AS.reconfigure ASconfig
@00:50:55.73 AS.seek 0.5ml
@00:51:24.74 delay 1 second
@00:51:25.76 AFV.select :holdingCoil
@00:51:25.87 Building Slug
@00:51:25.88 ATV.dial :ARV
@00:51:27.59 delay 2 seconds
@00:51:29.62 ARV.dial :air1
@00:51:30.01 delay 2 seconds
@00:51:32.07 AS.reconfigure ASslow2
@00:51:32.12 AS.seek 0.506ml
@00:51:38.63 delay 2 seconds
@00:51:40.66 ARV.dial :MM1
@00:51:41.27 delay 2 seconds
@00:51:43.30 Adding MM1
@00:51:43.36 AS.seek 0.524ml
@00:52:02.01 delay 25 seconds
@00:52:27.04 ARV.dial :PP1
@00:52:27.43 Adding PP1
@00:52:27.44 delay 2 seconds
@00:52:29.50 AS.seek 0.53ml
@00:52:36.06 delay 10 seconds
@00:52:46.10 ARV.dial :air1
@00:52:46.50 adding ARV air
@00:52:46.51 delay 2 seconds
@00:52:48.57 AS.seek 0.536ml
@00:52:54.87 delay 5 seconds
@00:52:59.90 ARV.dial :carrier
@00:53:00.31 delay 7 seconds
@00:53:07.39 AS.seek 0.594ml
@00:54:06.04 delay 5 seconds
@00:54:11.07 ATV.dial :elutionWater
@00:54:12.57 delay 2 seconds
@00:54:14.64 AS.seek 0.6ml
@00:54:20.82 delay 4 seconds
@00:54:24.85 ATV.dial ARV
@00:54:26.32 delay 2 seconds
@00:54:28.35 ARV.dial :carrier
@00:54:28.36 delay 2 seconds
@00:54:30.42 AS.seek 0.65ml
@00:55:21.17 delay 4 seconds
@00:55:25.20 ATV.dial PCR
@00:55:25.99 ASV.dial :waste1
@00:55:26.01 ARV.dial :air1
@00:55:26.40 Positioning Slug by 0.324ml
@00:55:26.46 AS.seek 0.326ml
@01:00:52.45 delay 5 seconds
@01:00:57.48 ATV.dial ARV
@01:00:58.26 ASV.dial :PCRlock
@01:00:58.65 delay 5 seconds
@01:01:03.68 Running AOPP1 Profile60CIQ1 thermocycling
@01:01:03.69 <PCR> PCR.program Profile60CIQ1
@01:01:03.80 Profile60CIQ1 should complete in < 1:56:15
@01:01:03.81 PCR.run 2:30:18.75
@02:40:05.36 PCR.off
@02:40:05.92 <MFBPCR> Running AOPP1 MeltProfile60 thermocycling
@02:40:05.93 <PCR> PCR.program MeltProfile60
@02:40:06.02 MeltProfile60 should complete in < 11:55
@02:40:06.03 PCR.run 19:53.75
@02:47:43.74 PCR.off
@02:47:45.06 <MFBPCR> PCR for AOPP1 completed -- closed /var/log/esp/AOPP121apr0600h.pcr
@02:47:45.12 Cleaning PCR
@02:47:45.13 AFV.select :carrierSolution
@02:47:45.24 delay 2 seconds
@02:47:47.26 AS.reconfigure ASconfig
@02:47:47.31 AS.seek 0.5ml
@02:47:57.62 delay 1 second
@02:47:58.64 AFV.select :holdingCoil
@02:47:58.75 ASV.dial :waste1
@02:47:59.14 ATV.dial :air3
@02:48:00.48 AS.reconfigure ASslow1
@02:48:00.53 AS.seek 0.51ml
@02:48:02.88 delay 2 seconds
@02:48:04.91 ATV.dial :bleach
@02:48:05.54 AS.seek 0.81ml
@02:49:06.12 delay 3 seconds
@02:49:09.15 ATV.dial :air3
@02:49:09.71 delay 3 seconds
@02:49:12.78 AS.seek 0.835ml
@02:49:18.04 delay 3 seconds
@02:49:21.07 ATV.dial :bleach
@02:49:21.70 AS.seek 0.82ml
@02:49:25.11 delay 3 seconds
@02:49:28.14 ARV.dial :waste
@02:49:28.75 delay 3 seconds
@02:49:31.78 ATV.dial :ARV
@02:49:32.68 AS.reconfigure ASconfig
@02:49:32.72 AS.seek 0.47ml
@02:49:53.14 AFV.select :carrierSolution
@02:49:53.25 delay 2 seconds
@02:49:55.27 AS.seek 1ml
@02:50:26.00 delay 1 second
@02:50:27.02 AFV.select :holdingCoil
@02:50:27.13 delay 1 second
@02:50:28.15 AS.reconfigure ASfast
@02:50:28.20 AS.seek 0ml
@02:50:55.54 AFV.select :carrierSolution
@02:50:55.65 delay 2 seconds
@02:50:57.67 AS.reconfigure ASconfig
@02:50:57.72 AS.seek 1ml
@02:51:55.45 delay 1 second
@02:51:56.47 AFV.select :holdingCoil
@02:51:56.58 delay 1 second
@02:51:57.60 AS.reconfigure ASfast
@02:51:57.65 AS.seek 0ml
@02:52:24.96 AFV.select :carrierSolution
@02:52:25.07 delay 2 seconds
@02:52:27.09 AS.reconfigure ASconfig
@02:52:27.14 AS.seek 1ml
@02:53:24.89 delay 1 second
@02:53:25.91 AFV.select :holdingCoil
@02:53:26.02 delay 1 second
@02:53:27.04 AS.reconfigure ASfast
@02:53:27.09 AS.seek 0ml
@02:53:54.42 ARV.dial between carrier and air1
@02:53:54.93 ATV.dial :air3
@02:53:56.26 AS.reconfigure ASslow1
@02:53:56.30 AS.seek 0.01ml
@02:53:58.64 delay 2 seconds
@02:54:00.67 ATV.dial :bleach
@02:54:01.27 AS.seek 0.81ml
@02:56:42.39 delay 3 seconds
@02:56:45.42 ATV.dial :air3
@02:56:45.98 delay 3 seconds
@02:56:49.05 AS.seek 0.835ml
@02:56:54.35 delay 3 seconds
@02:56:57.38 ATV.dial :bleach
@02:56:58.01 AS.seek 0.82ml
@02:57:01.32 delay 3 seconds
@02:57:04.35 ATV.dial PCR
@02:57:04.77 AS.reconfigure ASconfig
@02:57:04.81 AS.seek 0ml
@02:57:52.21 PCR.program :bleachProfile
@02:57:52.28 PCR program should complete in < 2:45
@02:57:52.29 PCR.run 10:00
@03:00:48.47 PCR.off
@03:00:48.49 AFV.select :carrierSolution
@03:00:48.60 delay 2 seconds
@03:00:50.62 AS.seek 1ml
@03:01:48.37 delay 1 second
@03:01:49.39 AFV.select :holdingCoil
@03:01:49.50 delay 1 second
@03:01:50.52 AS.reconfigure ASfast
@03:01:50.57 AS.seek 0ml
@03:02:17.90 delay 3 seconds
@03:02:20.92 AFV.select :carrierSolution
@03:02:21.03 delay 2 seconds
@03:02:23.05 AS.reconfigure ASconfig
@03:02:23.10 AS.seek 1ml
@03:03:20.84 delay 1 second
@03:03:21.86 AFV.select :holdingCoil
@03:03:21.97 delay 1 second
@03:03:22.99 AS.reconfigure ASfast
@03:03:23.04 AS.seek 0ml
@03:03:50.36 delay 3 seconds
@03:03:53.39 AFV.select :carrierSolution
@03:03:53.49 delay 2 seconds
@03:03:55.51 AS.reconfigure ASconfig
@03:03:55.56 AS.seek 1ml
@03:04:53.31 delay 1 second
@03:04:54.33 AFV.select :holdingCoil
@03:04:54.44 delay 1 second
@03:04:55.46 AS.reconfigure ASfast
@03:04:55.51 AS.seek 0ml
@03:05:22.81 delay 3 seconds
@03:05:25.83 AFV.select :carrierSolution
@03:05:25.94 delay 2 seconds
@03:05:27.96 AS.reconfigure ASconfig
@03:05:28.02 AS.seek 1ml
@03:06:25.76 delay 1 second
@03:06:26.78 AFV.select :holdingCoil
@03:06:26.89 delay 1 second
@03:06:27.91 AS.reconfigure ASfast
@03:06:27.96 AS.seek 0ml
@03:06:55.28 delay 3 seconds
@03:06:58.30 AFV.select :carrierSolution
@03:06:58.41 delay 2 seconds
@03:07:00.43 AS.reconfigure ASconfig
@03:07:00.48 AS.seek 1ml
@03:07:58.18 delay 1 second
@03:07:59.20 AFV.select :holdingCoil
@03:07:59.31 delay 1 second
@03:08:00.33 AS.reconfigure ASnorm
@03:08:00.38 AS.seek 0ml
@03:08:51.09 Setting up PCR for AARPP4 -- writing /var/log/esp/AARPP421apr0603h.pcr
@03:08:51.10 Building PCR Slug from elutionWater with MM2 mix, PP4 primer
@03:08:51.11 Priming with carrier
@03:08:51.12 ATV.dial ARV
@03:08:51.75 ARV.dial :carrier
@03:08:52.09 AS.seek 0.5ml
@03:09:17.65 AFV.select :carrierSolution
@03:09:17.76 delay 2 seconds
@03:09:19.78 AS.reconfigure ASconfig
@03:09:19.83 AS.seek 1ml
@03:09:48.84 delay 1 second
@03:09:49.87 AFV.select :holdingCoil
@03:09:49.97 delay 1 second
@03:09:50.99 ATV.dial :waste
@03:09:52.74 AS.reconfigure ASnorm
@03:09:52.78 AS.seek 0ml
@03:10:43.46 AFV.select :carrierSolution
@03:10:43.57 delay 2 seconds
@03:10:45.59 AS.reconfigure ASconfig
@03:10:45.64 AS.seek 0.5ml
@03:11:14.67 delay 1 second
@03:11:15.69 AFV.select :holdingCoil
@03:11:15.80 Building Slug
@03:11:15.81 ATV.dial :ARV
@03:11:17.53 delay 2 seconds
@03:11:19.56 ARV.dial :air1
@03:11:19.94 delay 2 seconds
@03:11:22.01 AS.reconfigure ASslow2
@03:11:22.06 AS.seek 0.506ml
@03:11:28.48 delay 2 seconds
@03:11:30.51 ARV.dial :MM2
@03:11:32.07 delay 2 seconds
@03:11:34.10 Adding MM2
@03:11:34.16 AS.seek 0.524ml
@03:11:52.73 delay 25 seconds
@03:12:17.76 ARV.dial :PP4
@03:12:18.16 Adding PP4
@03:12:18.17 delay 2 seconds
@03:12:20.23 AS.seek 0.53ml
@03:12:26.48 delay 10 seconds
@03:12:36.52 ARV.dial :air3
@03:12:36.93 adding ARV air
@03:12:36.94 delay 2 seconds
@03:12:39.00 AS.seek 0.536ml
@03:12:45.67 delay 5 seconds
@03:12:50.70 ARV.dial :carrier
@03:12:52.00 delay 7 seconds
@03:12:59.08 AS.seek 0.594ml
@03:13:57.70 delay 5 seconds
@03:14:02.73 ATV.dial :elutionWater
@03:14:04.23 delay 2 seconds
@03:14:06.30 AS.seek 0.6ml
@03:14:12.72 delay 4 seconds
@03:14:16.75 ATV.dial ARV
@03:14:18.23 delay 2 seconds
@03:14:20.26 ARV.dial :carrier
@03:14:20.27 delay 2 seconds
@03:14:22.33 AS.seek 0.65ml
@03:15:13.05 delay 4 seconds
@03:15:17.07 ATV.dial PCR
@03:15:17.86 ASV.dial :waste1
@03:15:17.88 ARV.dial :air1
@03:15:18.29 Positioning Slug by 0.324ml
@03:15:18.36 AS.seek 0.326ml
@03:20:44.29 delay 5 seconds
@03:20:49.32 ATV.dial ARV
@03:20:50.12 ASV.dial :PCRlock
@03:20:50.51 delay 5 seconds
@03:20:55.54 Running AARPP4 Profile58CIQ2 thermocycling
@03:20:55.55 <PCR> PCR.program Profile58CIQ2
@03:20:55.66 Profile58CIQ2 should complete in < 1:36:15
@03:20:55.67 PCR.run 2:05:18.75
@04:41:48.91 PCR.off
@04:41:49.86 <MFBPCR> Running AARPP4 MeltProfile58 thermocycling
@04:41:49.87 <PCR> PCR.program MeltProfile58
@04:41:49.96 MeltProfile58 should complete in < 12:33
@04:41:49.97 PCR.run 20:41.25
@04:49:35.01 PCR.off
@04:49:36.00 <MFBPCR> PCR for AARPP4 completed -- closed /var/log/esp/AARPP421apr0603h.pcr
@04:49:36.06 Cleaning PCR
@04:49:36.07 AFV.select :carrierSolution
@04:49:36.18 delay 2 seconds
@04:49:38.20 AS.reconfigure ASconfig
@04:49:38.25 AS.seek 0.5ml
@04:49:48.53 delay 1 second
@04:49:49.55 AFV.select :holdingCoil
@04:49:49.66 ASV.dial :waste1
@04:49:50.05 ATV.dial :air3
@04:49:51.39 AS.reconfigure ASslow1
@04:49:51.43 AS.seek 0.51ml
@04:49:53.70 delay 2 seconds
@04:49:55.73 ATV.dial :bleach
@04:49:56.34 AS.seek 0.81ml
@04:50:56.98 delay 3 seconds
@04:51:00.01 ATV.dial :air3
@04:51:00.58 delay 3 seconds
@04:51:03.65 AS.seek 0.835ml
@04:51:08.98 delay 3 seconds
@04:51:12.01 ATV.dial :bleach
@04:51:12.64 AS.seek 0.82ml
@04:51:15.97 delay 3 seconds
@04:51:19.00 ARV.dial :waste
@04:51:19.62 delay 3 seconds
@04:51:22.65 ATV.dial :ARV
@04:51:23.56 AS.reconfigure ASconfig
@04:51:23.60 AS.seek 0.47ml
@04:51:44.00 AFV.select :carrierSolution
@04:51:44.11 delay 2 seconds
@04:51:46.13 AS.seek 1ml
@04:52:16.89 delay 1 second
@04:52:17.91 AFV.select :holdingCoil
@04:52:18.02 delay 1 second
@04:52:19.04 AS.reconfigure ASfast
@04:52:19.09 AS.seek 0ml
@04:52:46.39 AFV.select :carrierSolution
@04:52:46.50 delay 2 seconds
@04:52:48.52 AS.reconfigure ASconfig
@04:52:48.57 AS.seek 1ml
@04:53:46.29 delay 1 second
@04:53:47.31 AFV.select :holdingCoil
@04:53:47.42 delay 1 second
@04:53:48.44 AS.reconfigure ASfast
@04:53:48.49 AS.seek 0ml
@04:54:15.80 AFV.select :carrierSolution
@04:54:15.91 delay 2 seconds
@04:54:17.93 AS.reconfigure ASconfig
@04:54:17.98 AS.seek 1ml
@04:55:15.70 delay 1 second
@04:55:16.72 AFV.select :holdingCoil
@04:55:16.83 delay 1 second
@04:55:17.85 AS.reconfigure ASfast
@04:55:17.90 AS.seek 0ml
@04:55:45.19 ARV.dial between carrier and air1
@04:55:45.70 ATV.dial :air3
@04:55:47.04 AS.reconfigure ASslow1
@04:55:47.09 AS.seek 0.01ml
@04:55:49.36 delay 2 seconds
@04:55:51.39 ATV.dial :bleach
@04:55:52.01 AS.seek 0.81ml
@04:58:33.12 delay 3 seconds
@04:58:36.15 ATV.dial :air3
@04:58:36.72 delay 3 seconds
@04:58:39.79 AS.seek 0.835ml
@04:58:45.11 delay 3 seconds
@04:58:48.14 ATV.dial :bleach
@04:58:48.76 AS.seek 0.82ml
@04:58:52.05 delay 3 seconds
@04:58:55.08 ATV.dial PCR
@04:58:55.48 AS.reconfigure ASconfig
@04:58:55.52 AS.seek 0ml
@04:59:42.92 PCR.program :bleachProfile
@04:59:42.99 PCR program should complete in < 2:45
@04:59:43.00 PCR.run 10:00
@05:02:39.19 PCR.off
@05:02:39.21 AFV.select :carrierSolution
@05:02:39.32 delay 2 seconds
@05:02:41.34 AS.seek 1ml
@05:03:39.08 delay 1 second
@05:03:40.10 AFV.select :holdingCoil
@05:03:40.21 delay 1 second
@05:03:41.23 AS.reconfigure ASfast
@05:03:41.28 AS.seek 0ml
@05:04:08.62 delay 3 seconds
@05:04:11.64 AFV.select :carrierSolution
@05:04:11.75 delay 2 seconds
@05:04:13.77 AS.reconfigure ASconfig
@05:04:13.83 AS.seek 1ml
@05:05:11.56 delay 1 second
@05:05:12.58 AFV.select :holdingCoil
@05:05:12.69 delay 1 second
@05:05:13.71 AS.reconfigure ASfast
@05:05:13.76 AS.seek 0ml
@05:05:41.09 delay 3 seconds
@05:05:44.11 AFV.select :carrierSolution
@05:05:44.22 delay 2 seconds
@05:05:46.24 AS.reconfigure ASconfig
@05:05:46.30 AS.seek 1ml
@05:06:44.03 delay 1 second
@05:06:45.05 AFV.select :holdingCoil
@05:06:45.16 delay 1 second
@05:06:46.18 AS.reconfigure ASfast
@05:06:46.23 AS.seek 0ml
@05:07:13.56 delay 3 seconds
@05:07:16.58 AFV.select :carrierSolution
@05:07:16.69 delay 2 seconds
@05:07:18.71 AS.reconfigure ASconfig
@05:07:18.77 AS.seek 1ml
@05:08:16.51 delay 1 second
@05:08:17.53 AFV.select :holdingCoil
@05:08:17.64 delay 1 second
@05:08:18.66 AS.reconfigure ASfast
@05:08:18.71 AS.seek 0ml
@05:08:46.03 delay 3 seconds
@05:08:49.05 AFV.select :carrierSolution
@05:08:49.16 delay 2 seconds
@05:08:51.18 AS.reconfigure ASconfig
@05:08:51.24 AS.seek 1ml
@05:09:49.03 delay 1 second
@05:09:50.05 AFV.select :holdingCoil
@05:09:50.16 delay 1 second
@05:09:51.18 AS.reconfigure ASnorm
@05:09:51.23 AS.seek 0ml
@05:10:41.97 Setting up PCR for AALPP3 -- writing /var/log/esp/AALPP321apr0605h.pcr
@05:10:41.98 Building PCR Slug from elutionWater with MM2 mix, PP3 primer
@05:10:41.99 Priming with carrier
@05:10:42.00 ATV.dial ARV
@05:10:42.64 ARV.dial :carrier
@05:10:42.98 AS.seek 0.5ml
@05:11:08.51 AFV.select :carrierSolution
@05:11:08.62 delay 2 seconds
@05:11:10.64 AS.reconfigure ASconfig
@05:11:10.69 AS.seek 1ml
@05:11:39.70 delay 1 second
@05:11:40.72 AFV.select :holdingCoil
@05:11:40.83 delay 1 second
@05:11:41.85 ATV.dial :waste
@05:11:43.62 AS.reconfigure ASnorm
@05:11:43.66 AS.seek 0ml
@05:12:34.37 AFV.select :carrierSolution
@05:12:34.48 delay 2 seconds
@05:12:36.50 AS.reconfigure ASconfig
@05:12:36.55 AS.seek 0.5ml
@05:13:05.55 delay 1 second
@05:13:06.57 AFV.select :holdingCoil
@05:13:06.68 Building Slug
@05:13:06.69 ATV.dial :ARV
@05:13:08.83 delay 2 seconds
@05:13:10.86 ARV.dial :air1
@05:13:11.25 delay 2 seconds
@05:13:13.32 AS.reconfigure ASslow2
@05:13:13.37 AS.seek 0.506ml
@05:13:19.92 delay 2 seconds
@05:13:21.95 ARV.dial :MM2
@05:13:23.51 delay 2 seconds
@05:13:25.54 Adding MM2
@05:13:25.60 AS.seek 0.524ml
@05:13:44.00 delay 25 seconds
@05:14:09.03 ARV.dial :PP3
@05:14:09.43 Adding PP3
@05:14:09.44 delay 2 seconds
@05:14:11.51 AS.seek 0.53ml
@05:14:17.86 delay 10 seconds
@05:14:27.90 ARV.dial :air2
@05:14:28.31 adding ARV air
@05:14:28.32 delay 2 seconds
@05:14:30.38 AS.seek 0.536ml
@05:14:36.67 delay 5 seconds
@05:14:41.70 ARV.dial :carrier
@05:14:43.03 delay 7 seconds
@05:14:50.11 AS.seek 0.594ml
@05:15:48.61 delay 5 seconds
@05:15:53.64 ATV.dial :elutionWater
@05:15:55.12 delay 2 seconds
@05:15:57.19 AS.seek 0.6ml
@05:16:03.53 delay 4 seconds
@05:16:07.56 ATV.dial ARV
@05:16:09.04 delay 2 seconds
@05:16:11.07 ARV.dial :carrier
@05:16:11.08 delay 2 seconds
@05:16:13.14 AS.seek 0.65ml
@05:17:03.90 delay 4 seconds
@05:17:07.93 ATV.dial PCR
@05:17:08.72 ASV.dial :waste1
@05:17:08.74 ARV.dial :air1
@05:17:09.13 Positioning Slug by 0.324ml
@05:17:09.20 AS.seek 0.326ml
@05:22:35.61 delay 5 seconds
@05:22:40.64 ATV.dial ARV
@05:22:41.44 ASV.dial :PCRlock
@05:22:41.83 delay 5 seconds
@05:22:46.86 Running AALPP3 Profile58CIQ2 thermocycling
@05:22:46.87 <PCR> PCR.program Profile58CIQ2
@05:22:46.97 Profile58CIQ2 should complete in < 1:36:15
@05:22:46.98 PCR.run 2:05:18.75
@06:43:41.36 PCR.off
@06:43:41.92 <MFBPCR> Running AALPP3 MeltProfile58 thermocycling
@06:43:41.94 <PCR> PCR.program MeltProfile58
@06:43:42.03 MeltProfile58 should complete in < 12:33
@06:43:42.04 PCR.run 20:41.25
@06:51:20.63 PCR.off
@06:51:21.62 <MFBPCR> PCR for AALPP3 completed -- closed /var/log/esp/AALPP321apr0605h.pcr
@06:51:21.68 Cleaning PCR
@06:51:21.69 AFV.select :carrierSolution
@06:51:21.79 delay 2 seconds
@06:51:23.81 AS.reconfigure ASconfig
@06:51:23.86 AS.seek 0.5ml
@06:51:34.16 delay 1 second
@06:51:35.18 AFV.select :holdingCoil
@06:51:35.29 ASV.dial :waste1
@06:51:35.67 ATV.dial :air3
@06:51:37.00 AS.reconfigure ASslow1
@06:51:37.04 AS.seek 0.51ml
@06:51:39.31 delay 2 seconds
@06:51:41.34 ATV.dial :bleach
@06:51:41.95 AS.seek 0.81ml
@06:52:42.61 delay 3 seconds
@06:52:45.64 ATV.dial :air3
@06:52:46.20 delay 3 seconds
@06:52:49.27 AS.seek 0.835ml
@06:52:54.60 delay 3 seconds
@06:52:57.63 ATV.dial :bleach
@06:52:58.26 AS.seek 0.82ml
@06:53:01.70 delay 3 seconds
@06:53:04.73 ARV.dial :waste
@06:53:05.34 delay 3 seconds
@06:53:08.37 ATV.dial :ARV
@06:53:09.28 AS.reconfigure ASconfig
@06:53:09.32 AS.seek 0.47ml
@06:53:29.69 AFV.select :carrierSolution
@06:53:29.80 delay 2 seconds
@06:53:31.82 AS.seek 1ml
@06:54:02.56 delay 1 second
@06:54:03.58 AFV.select :holdingCoil
@06:54:03.71 delay 1 second
@06:54:04.74 AS.reconfigure ASfast
@06:54:04.81 AS.seek 0ml
@06:54:32.14 AFV.select :carrierSolution
@06:54:32.25 delay 2 seconds
@06:54:34.27 AS.reconfigure ASconfig
@06:54:34.32 AS.seek 1ml
@06:55:32.06 delay 1 second
@06:55:33.08 AFV.select :holdingCoil
@06:55:33.19 delay 1 second
@06:55:34.21 AS.reconfigure ASfast
@06:55:34.66 AS.seek 0ml
@06:56:01.97 AFV.select :carrierSolution
@06:56:02.08 delay 2 seconds
@06:56:04.10 AS.reconfigure ASconfig
@06:56:04.15 AS.seek 1ml
@06:57:01.89 delay 1 second
@06:57:02.91 AFV.select :holdingCoil
@06:57:03.02 delay 1 second
@06:57:04.04 AS.reconfigure ASfast
@06:57:04.09 AS.seek 0ml
@06:57:31.41 ARV.dial between carrier and air1
@06:57:31.92 ATV.dial :air3
@06:57:33.25 AS.reconfigure ASslow1
@06:57:33.29 AS.seek 0.01ml
@06:57:35.72 delay 2 seconds
@06:57:37.75 ATV.dial :bleach
@06:57:38.37 AS.seek 0.81ml
@07:00:19.52 delay 3 seconds
@07:00:22.55 ATV.dial :air3
@07:00:23.11 delay 3 seconds
@07:00:26.18 AS.seek 0.835ml
@07:00:31.56 delay 3 seconds
@07:00:34.59 ATV.dial :bleach
@07:00:35.22 AS.seek 0.82ml
@07:00:38.52 delay 3 seconds
@07:00:41.55 ATV.dial PCR
@07:00:41.95 AS.reconfigure ASconfig
@07:00:41.99 AS.seek 0ml
@07:01:29.40 PCR.program :bleachProfile
@07:01:29.49 PCR program should complete in < 2:45
@07:01:29.51 PCR.run 10:00
@07:04:25.31 PCR.off
@07:04:25.33 AFV.select :carrierSolution
@07:04:25.44 delay 2 seconds
@07:04:27.46 AS.seek 1ml
@07:05:25.20 delay 1 second
@07:05:26.22 AFV.select :holdingCoil
@07:05:26.33 delay 1 second
@07:05:27.35 AS.reconfigure ASfast
@07:05:27.40 AS.seek 0ml
@07:05:54.72 delay 3 seconds
@07:05:57.74 AFV.select :carrierSolution
@07:05:57.85 delay 2 seconds
@07:05:59.87 AS.reconfigure ASconfig
@07:05:59.92 AS.seek 1ml
@07:06:57.66 delay 1 second
@07:06:58.68 AFV.select :holdingCoil
@07:06:58.79 delay 1 second
@07:06:59.81 AS.reconfigure ASfast
@07:06:59.86 AS.seek 0ml
@07:07:27.20 delay 3 seconds
@07:07:30.22 AFV.select :carrierSolution
@07:07:30.33 delay 2 seconds
@07:07:32.35 AS.reconfigure ASconfig
@07:07:32.41 AS.seek 1ml
@07:08:30.16 delay 1 second
@07:08:31.18 AFV.select :holdingCoil
@07:08:31.29 delay 1 second
@07:08:32.31 AS.reconfigure ASfast
@07:08:32.36 AS.seek 0ml
@07:08:59.69 delay 3 seconds
@07:09:02.71 AFV.select :carrierSolution
@07:09:02.82 delay 2 seconds
@07:09:04.84 AS.reconfigure ASconfig
@07:09:04.89 AS.seek 1ml
@07:10:02.61 delay 1 second
@07:10:04.03 AFV.select :holdingCoil
@07:10:04.14 delay 1 second
@07:10:05.16 AS.reconfigure ASfast
@07:10:05.21 AS.seek 0ml
@07:10:32.53 delay 3 seconds
@07:10:35.55 AFV.select :carrierSolution
@07:10:35.66 delay 2 seconds
@07:10:37.68 AS.reconfigure ASconfig
@07:10:37.74 AS.seek 1ml
@07:11:35.53 delay 1 second
@07:11:36.55 AFV.select :holdingCoil
@07:11:36.66 delay 1 second
@07:11:37.68 AS.reconfigure ASnorm
@07:11:37.73 AS.seek 0ml
@07:12:28.45 Setting up PCR for ACOPP5 -- writing /var/log/esp/ACOPP521apr0607h.pcr
@07:12:28.46 Building PCR Slug from elutionWater with MM3 mix, PP5 primer
@07:12:28.47 Priming with carrier
@07:12:28.48 ATV.dial ARV
@07:12:29.11 ARV.dial :carrier
@07:12:29.45 AS.seek 0.5ml
@07:12:55.02 AFV.select :carrierSolution
@07:12:55.13 delay 2 seconds
@07:12:57.15 AS.reconfigure ASconfig
@07:12:57.20 AS.seek 1ml
@07:13:26.19 delay 1 second
@07:13:27.22 AFV.select :holdingCoil
@07:13:27.32 delay 1 second
@07:13:28.34 ATV.dial :waste
@07:13:30.10 AS.reconfigure ASnorm
@07:13:30.14 AS.seek 0ml
@07:14:20.86 AFV.select :carrierSolution
@07:14:20.97 delay 2 seconds
@07:14:22.99 AS.reconfigure ASconfig
@07:14:23.04 AS.seek 0.5ml
@07:14:52.06 delay 1 second
@07:14:53.08 AFV.select :holdingCoil
@07:14:53.19 Building Slug
@07:14:53.20 ATV.dial :ARV
@07:14:54.92 delay 2 seconds
@07:14:56.95 ARV.dial :air1
@07:14:57.33 delay 2 seconds
@07:14:59.40 AS.reconfigure ASslow2
@07:14:59.45 AS.seek 0.506ml
@07:15:06.31 delay 2 seconds
@07:15:08.36 ARV.dial :MM3
@07:15:09.46 delay 2 seconds
@07:15:11.49 Adding MM3
@07:15:11.61 AS.seek 0.524ml
@07:15:30.64 delay 25 seconds
@07:15:55.67 ARV.dial :PP5
@07:15:56.06 Adding PP5
@07:15:56.08 delay 2 seconds
@07:15:58.14 AS.seek 0.53ml
@07:16:05.00 delay 10 seconds
@07:16:15.04 ARV.dial :air3
@07:16:15.43 adding ARV air
@07:16:15.44 delay 2 seconds
@07:16:17.50 AS.seek 0.536ml
@07:16:24.17 delay 5 seconds
@07:16:29.20 ARV.dial :carrier
@07:16:30.50 delay 7 seconds
@07:16:37.57 AS.seek 0.594ml
@07:17:36.38 delay 5 seconds
@07:17:41.41 ATV.dial :elutionWater
@07:17:42.91 delay 2 seconds
@07:17:44.98 AS.seek 0.6ml
@07:17:51.24 delay 4 seconds
@07:17:55.27 ATV.dial ARV
@07:17:56.76 delay 2 seconds
@07:17:58.79 ARV.dial :carrier
@07:17:58.80 delay 2 seconds
@07:18:00.86 AS.seek 0.65ml
@07:18:51.28 delay 4 seconds
@07:18:55.31 ATV.dial PCR
@07:18:56.10 ASV.dial :waste1
@07:18:56.12 ARV.dial :air1
@07:18:56.53 Positioning Slug by 0.324ml
@07:18:56.60 AS.seek 0.326ml
@07:24:23.06 delay 5 seconds
@07:24:28.09 ATV.dial ARV
@07:24:28.89 ASV.dial :PCRlock
@07:24:29.28 delay 5 seconds
@07:24:34.31 Running ACOPP5 ProfileIPc thermocycling
@07:24:34.32 <PCR> PCR.program ProfileIPc
@07:24:34.43 ProfileIPc should complete in < 1:26:35
@07:24:34.44 PCR.run 1:53:13.75
@08:34:39.36 PCR.off
@08:34:39.91 <MFBPCR> PCR for ACOPP5 completed -- closed /var/log/esp/ACOPP521apr0607h.pcr
@08:34:39.97 Cleaning PCR
@08:34:39.98 AFV.select :carrierSolution
@08:34:40.08 delay 2 seconds
@08:34:42.10 AS.reconfigure ASconfig
@08:34:42.15 AS.seek 0.5ml
@08:34:52.47 delay 1 second
@08:34:53.49 AFV.select :holdingCoil
@08:34:53.60 ASV.dial :waste1
@08:34:53.99 ATV.dial :air3
@08:34:55.70 AS.reconfigure ASslow1
@08:34:55.74 AS.seek 0.51ml
@08:34:58.08 delay 2 seconds
@08:35:00.11 ATV.dial :bleach
@08:35:00.72 AS.seek 0.81ml
@08:36:01.31 delay 3 seconds
@08:36:04.34 ATV.dial :air3
@08:36:04.89 delay 3 seconds
@08:36:07.96 AS.seek 0.835ml
@08:36:13.25 delay 3 seconds
@08:36:16.28 ATV.dial :bleach
@08:36:16.89 AS.seek 0.82ml
@08:36:20.24 delay 3 seconds
@08:36:23.27 ARV.dial :waste
@08:36:23.89 delay 3 seconds
@08:36:26.92 ATV.dial :ARV
@08:36:27.83 AS.reconfigure ASconfig
@08:36:27.87 AS.seek 0.47ml
@08:36:48.25 AFV.select :carrierSolution
@08:36:48.36 delay 2 seconds
@08:36:50.38 AS.seek 1ml
@08:37:21.11 delay 1 second
@08:37:22.13 AFV.select :holdingCoil
@08:37:22.24 delay 1 second
@08:37:23.26 AS.reconfigure ASfast
@08:37:23.31 AS.seek 0ml
@08:37:50.64 AFV.select :carrierSolution
@08:37:50.75 delay 2 seconds
@08:37:52.77 AS.reconfigure ASconfig
@08:37:52.82 AS.seek 1ml
@08:38:50.55 delay 1 second
@08:38:51.57 AFV.select :holdingCoil
@08:38:51.68 delay 1 second
@08:38:52.70 AS.reconfigure ASfast
@08:38:52.75 AS.seek 0ml
@08:39:20.05 AFV.select :carrierSolution
@08:39:20.16 delay 2 seconds
@08:39:22.18 AS.reconfigure ASconfig
@08:39:22.23 AS.seek 1ml
@08:40:19.97 delay 1 second
@08:40:20.99 AFV.select :holdingCoil
@08:40:21.10 delay 1 second
@08:40:22.12 AS.reconfigure ASfast
@08:40:22.17 AS.seek 0ml
@08:40:49.50 ARV.dial between carrier and air1
@08:40:50.03 ATV.dial :air3
@08:40:51.34 AS.reconfigure ASslow1
@08:40:51.38 AS.seek 0.01ml
@08:40:53.66 delay 2 seconds
@08:40:55.69 ATV.dial :bleach
@08:40:56.31 AS.seek 0.81ml
@08:43:37.42 delay 3 seconds
@08:43:40.45 ATV.dial :air3
@08:43:41.02 delay 3 seconds
@08:43:44.09 AS.seek 0.835ml
@08:43:49.40 delay 3 seconds
@08:43:52.43 ATV.dial :bleach
@08:43:53.06 AS.seek 0.82ml
@08:43:56.36 delay 3 seconds
@08:43:59.39 ATV.dial PCR
@08:43:59.79 AS.reconfigure ASconfig
@08:43:59.83 AS.seek 0ml
@08:44:47.24 PCR.program :bleachProfile
@08:44:47.31 PCR program should complete in < 2:45
@08:44:47.32 PCR.run 10:00
@08:47:43.50 PCR.off
@08:47:43.53 AFV.select :carrierSolution
@08:47:43.64 delay 2 seconds
@08:47:45.66 AS.seek 1ml
@08:48:43.41 delay 1 second
@08:48:44.43 AFV.select :holdingCoil
@08:48:44.54 delay 1 second
@08:48:45.56 AS.reconfigure ASfast
@08:48:45.61 AS.seek 0ml
@08:49:12.94 delay 3 seconds
@08:49:15.96 AFV.select :carrierSolution
@08:49:16.07 delay 2 seconds
@08:49:18.09 AS.reconfigure ASconfig
@08:49:18.14 AS.seek 1ml
@08:50:15.88 delay 1 second
@08:50:16.90 AFV.select :holdingCoil
@08:50:17.01 delay 1 second
@08:50:18.03 AS.reconfigure ASfast
@08:50:18.08 AS.seek 0ml
@08:50:45.41 delay 3 seconds
@08:50:48.43 AFV.select :carrierSolution
@08:50:48.54 delay 2 seconds
@08:50:50.56 AS.reconfigure ASconfig
@08:50:50.61 AS.seek 1ml
@08:51:48.38 delay 1 second
@08:51:49.40 AFV.select :holdingCoil
@08:51:49.51 delay 1 second
@08:51:50.53 AS.reconfigure ASfast
@08:51:50.58 AS.seek 0ml
@08:52:17.91 delay 3 seconds
@08:52:20.93 AFV.select :carrierSolution
@08:52:21.04 delay 2 seconds
@08:52:23.06 AS.reconfigure ASconfig
@08:52:23.11 AS.seek 1ml
@08:53:20.93 delay 1 second
@08:53:21.99 AFV.select :holdingCoil
@08:53:22.10 delay 1 second
@08:53:23.12 AS.reconfigure ASfast
@08:53:23.17 AS.seek 0ml
@08:53:50.49 delay 3 seconds
@08:53:53.51 AFV.select :carrierSolution
@08:53:53.62 delay 2 seconds
@08:53:55.64 AS.reconfigure ASconfig
@08:53:55.69 AS.seek 1ml
@08:54:53.44 delay 1 second
@08:54:54.46 AFV.select :holdingCoil
@08:54:54.57 delay 1 second
@08:54:55.59 AS.reconfigure ASnorm
@08:54:55.64 AS.seek 0ml
@08:55:46.36 Setting up PCR for AIPc -- writing /var/log/esp/AIPc21apr0608h.pcr
@08:55:46.37 Building PCR Slug from elutionWater with MM3 mix, PP6 primer
@08:55:46.38 Priming with carrier
@08:55:46.39 ATV.dial ARV
@08:55:47.02 ARV.dial :carrier
@08:55:47.36 AS.seek 0.5ml
@08:56:12.94 AFV.select :carrierSolution
@08:56:13.05 delay 2 seconds
@08:56:15.07 AS.reconfigure ASconfig
@08:56:15.12 AS.seek 1ml
@08:56:44.13 delay 1 second
@08:56:45.15 AFV.select :holdingCoil
@08:56:45.26 delay 1 second
@08:56:46.28 ATV.dial :waste
@08:56:48.03 AS.reconfigure ASnorm
@08:56:48.07 AS.seek 0ml
@08:57:38.77 AFV.select :carrierSolution
@08:57:38.88 delay 2 seconds
@08:57:40.90 AS.reconfigure ASconfig
@08:57:40.95 AS.seek 0.5ml
@08:58:09.97 delay 1 second
@08:58:10.99 AFV.select :holdingCoil
@08:58:11.10 Building Slug
@08:58:11.11 ATV.dial :ARV
@08:58:12.91 delay 2 seconds
@08:58:14.94 ARV.dial :air1
@08:58:15.31 delay 2 seconds
@08:58:17.38 AS.reconfigure ASslow2
@08:58:17.43 AS.seek 0.506ml
@08:58:23.74 delay 2 seconds
@08:58:25.77 ARV.dial :MM3
@08:58:26.85 delay 2 seconds
@08:58:28.88 Adding MM3
@08:58:28.94 AS.seek 0.524ml
@08:58:47.47 delay 25 seconds
@08:59:12.50 ARV.dial :PP6
@08:59:12.88 Adding PP6
@08:59:12.90 delay 2 seconds
@08:59:14.96 AS.seek 0.53ml
@08:59:21.45 delay 10 seconds
@08:59:31.49 ARV.dial :air1
@08:59:32.35 adding ARV air
@08:59:32.37 delay 2 seconds
@08:59:34.44 AS.seek 0.536ml
@08:59:40.75 delay 5 seconds
@08:59:45.78 ARV.dial :carrier
@08:59:46.19 delay 7 seconds
@08:59:53.26 AS.seek 0.594ml
@09:00:51.91 delay 5 seconds
@09:00:56.94 ATV.dial :elutionWater
@09:00:58.46 delay 2 seconds
@09:01:00.53 AS.seek 0.6ml
@09:01:06.72 delay 4 seconds
@09:01:10.75 ATV.dial ARV
@09:01:12.22 delay 2 seconds
@09:01:14.25 ARV.dial :carrier
@09:01:14.26 delay 2 seconds
@09:01:16.32 AS.seek 0.65ml
@09:02:07.03 delay 4 seconds
@09:02:11.06 ATV.dial PCR
@09:02:11.85 ASV.dial :waste1
@09:02:11.87 ARV.dial :air1
@09:02:12.26 Positioning Slug by 0.324ml
@09:02:12.33 AS.seek 0.326ml
@09:07:38.77 delay 5 seconds
@09:07:43.80 ATV.dial ARV
@09:07:44.60 ASV.dial :PCRlock
@09:07:45.42 delay 5 seconds
@09:07:50.45 Running AIPc ProfileIPc thermocycling
@09:07:50.46 <PCR> PCR.program ProfileIPc
@09:07:50.57 ProfileIPc should complete in < 1:26:35
@09:07:50.58 PCR.run 1:53:13.75
@10:17:11.26 PCR.off
@10:17:11.82 <MFBPCR> PCR for AIPc completed -- closed /var/log/esp/AIPc21apr0608h.pcr
@10:17:11.88 Cleaning PCR
@10:17:11.89 AFV.select :carrierSolution
@10:17:12.00 delay 2 seconds
@10:17:14.02 AS.reconfigure ASconfig
@10:17:14.07 AS.seek 0.5ml
@10:17:24.38 delay 1 second
@10:17:25.40 AFV.select :holdingCoil
@10:17:25.51 ASV.dial :waste1
@10:17:25.89 ATV.dial :air3
@10:17:27.23 AS.reconfigure ASslow1
@10:17:27.27 AS.seek 0.51ml
@10:17:29.54 delay 2 seconds
@10:17:31.56 ATV.dial :bleach
@10:17:32.17 AS.seek 0.81ml
@10:18:32.81 delay 3 seconds
@10:18:35.84 ATV.dial :air3
@10:18:36.39 delay 3 seconds
@10:18:39.46 AS.seek 0.835ml
@10:18:44.79 delay 3 seconds
@10:18:47.82 ATV.dial :bleach
@10:18:48.45 AS.seek 0.82ml
@10:18:51.75 delay 3 seconds
@10:18:54.78 ARV.dial :waste
@10:18:55.39 delay 3 seconds
@10:18:58.42 ATV.dial :ARV
@10:18:59.33 AS.reconfigure ASconfig
@10:18:59.37 AS.seek 0.47ml
@10:19:19.75 AFV.select :carrierSolution
@10:19:19.86 delay 2 seconds
@10:19:21.88 AS.seek 1ml
@10:19:52.61 delay 1 second
@10:19:53.63 AFV.select :holdingCoil
@10:19:53.74 delay 1 second
@10:19:54.76 AS.reconfigure ASfast
@10:19:54.81 AS.seek 0ml
@10:20:22.14 AFV.select :carrierSolution
@10:20:22.25 delay 2 seconds
@10:20:24.27 AS.reconfigure ASconfig
@10:20:24.32 AS.seek 1ml
@10:21:22.10 delay 1 second
@10:21:23.12 AFV.select :holdingCoil
@10:21:23.23 delay 1 second
@10:21:24.25 AS.reconfigure ASfast
@10:21:24.30 AS.seek 0ml
@10:21:51.61 AFV.select :carrierSolution
@10:21:51.72 delay 2 seconds
@10:21:53.74 AS.reconfigure ASconfig
@10:21:53.79 AS.seek 1ml
@10:22:51.53 delay 1 second
@10:22:52.55 AFV.select :holdingCoil
@10:22:52.66 delay 1 second
@10:22:53.68 AS.reconfigure ASfast
@10:22:53.73 AS.seek 0ml
@10:23:21.05 ARV.dial between carrier and air1
@10:23:21.56 ATV.dial :air3
@10:23:22.91 AS.reconfigure ASslow1
@10:23:22.95 AS.seek 0.01ml
@10:23:25.35 delay 2 seconds
@10:23:27.38 ATV.dial :bleach
@10:23:28.00 AS.seek 0.81ml
@10:26:09.13 delay 3 seconds
@10:26:12.16 ATV.dial :air3
@10:26:12.71 delay 3 seconds
@10:26:15.78 AS.seek 0.835ml
@10:26:21.08 delay 3 seconds
@10:26:24.11 ATV.dial :bleach
@10:26:24.73 AS.seek 0.82ml
@10:26:28.14 delay 3 seconds
@10:26:31.17 ATV.dial PCR
@10:26:31.57 AS.reconfigure ASconfig
@10:26:31.61 AS.seek 0ml
@10:27:19.02 PCR.program :bleachProfile
@10:27:19.09 PCR program should complete in < 2:45
@10:27:19.10 PCR.run 10:00
@10:30:15.30 PCR.off
@10:30:15.32 AFV.select :carrierSolution
@10:30:15.43 delay 2 seconds
@10:30:17.45 AS.seek 1ml
@10:31:15.22 delay 1 second
@10:31:16.24 AFV.select :holdingCoil
@10:31:16.35 delay 1 second
@10:31:17.37 AS.reconfigure ASfast
@10:31:17.42 AS.seek 0ml
@10:31:44.75 delay 3 seconds
@10:31:47.77 AFV.select :carrierSolution
@10:31:47.88 delay 2 seconds
@10:31:49.90 AS.reconfigure ASconfig
@10:31:49.95 AS.seek 1ml
@10:32:47.74 delay 1 second
@10:32:48.76 AFV.select :holdingCoil
@10:32:48.87 delay 1 second
@10:32:49.89 AS.reconfigure ASfast
@10:32:49.94 AS.seek 0ml
@10:33:17.30 delay 3 seconds
@10:33:20.32 AFV.select :carrierSolution
@10:33:20.43 delay 2 seconds
@10:33:22.45 AS.reconfigure ASconfig
@10:33:22.51 AS.seek 1ml
@10:34:20.24 delay 1 second
@10:34:21.26 AFV.select :holdingCoil
@10:34:21.37 delay 1 second
@10:34:22.39 AS.reconfigure ASfast
@10:34:22.44 AS.seek 0ml
@10:34:49.78 delay 3 seconds
@10:34:52.80 AFV.select :carrierSolution
@10:34:52.91 delay 2 seconds
@10:34:54.93 AS.reconfigure ASconfig
@10:34:54.98 AS.seek 1ml
@10:35:52.72 delay 1 second
@10:35:53.74 AFV.select :holdingCoil
@10:35:53.85 delay 1 second
@10:35:54.87 AS.reconfigure ASfast
@10:35:54.92 AS.seek 0ml
@10:36:22.25 delay 3 seconds
@10:36:25.27 AFV.select :carrierSolution
@10:36:25.38 delay 2 seconds
@10:36:27.40 AS.reconfigure ASconfig
@10:36:27.45 AS.seek 1ml
@10:37:25.22 delay 1 second
@10:37:26.24 AFV.select :holdingCoil
@10:37:26.35 delay 1 second
@10:37:27.37 AS.reconfigure ASnorm
@10:37:27.42 AS.seek 0ml
@14:04:32.35CEST06-Apr-21 <MAIN> ESP2 v3.30 I2C Gateway v3.06 for core bus
@14:04:34.41 
Configuration for mer 7/20/20 brent@mbari.org
/home/esp/esp2/type/shallow/mer/configure.rb
@14:04:34.50 
Configuration for MFB3 by preston@mbari.org
/home/esp/esp2/type/mfb/MFB3/configure.rb
@14:04:38.79 Polling Can every 10:00
@14:04:40.49 CS.configure :default
@14:04:40.53 CS.hold
@14:04:40.60 CS.configure :default
@14:04:40.68 PS.configure :default
@14:04:40.76 SS.configure :default
@14:04:40.80 SS.hold
@14:04:40.84 SS.configure :default
@14:04:40.92 CC.configure :default
@14:04:40.96 CC.hold
@14:04:41.01 CC.configure :default
@14:04:41.09 PC.configure :default
@14:04:41.12 PC.hold
@14:04:41.19 PC.configure :default
@14:04:41.29 Forearm.configure :loose
@14:04:41.32 Forearm.hold
@14:04:41.37 Forearm.configure :loose
@14:04:41.44 Elbow.configure :loose
@14:04:41.48 Elbow.hold
@14:04:41.53 Elbow.configure :loose
@14:04:41.62 SC.configure :loose
@14:04:41.66 SC.hold
@14:04:41.72 SC.configure :loose
@14:04:41.80 SE.configure :default
@14:04:41.84 SE.hold
@14:04:41.89 SE.configure :default
@14:04:41.93 CH.coast
@14:04:41.97 CH.configure :default
@14:04:42.00 PH.coast
@14:04:42.05 PH.configure :default
@14:04:42.15 SPE.coast
@14:04:42.19 SPE.configure SPEconfig
@14:04:42.26 AS.configure ASconfig
@14:04:42.30 AS.hold
@14:04:42.34 AS.configure ASconfig
@14:04:42.39 PCR.off
@14:04:42.42 PCR.pidtemp= 50,0.1,0,300,25,25
@14:04:42.45 PCR.caltemp= 2.5051,0.0372,0
@14:04:42.48 All dwarf controllers running firmware version 3.75
@14:04:42.66 Cleanly exited at 14:03:57CEST06-Apr-21
@14:04:42.69 Loading /home/esp/esp2/mission/GENOMAP.rb mission
@14:04:42.84 <MFBPCR> Extracting Nucleic Acid from extSrc
@14:04:42.86 SPE.hold 2:46:40,55C
@14:04:42.91 ASV.dial :SPEdelivery
@14:04:43.31 delay 20 seconds
@14:04:53.07 <bspe> Session Begun
@14:05:03.34 <MFBPCR> AFV.select :holdingCoil
@14:05:03.46 ATV.dial :air2
@14:05:04.73 AS.reconfigure ASslow1
@14:05:04.79 AS.seek 0.07ml
@14:05:19.11 delay 3 seconds
@14:05:22.14 ATV.dial :extSrc
@14:05:23.67 AS.seek 0.47ml
@14:06:44.41 delay 5 seconds
@14:06:49.49 Solid Phase Extraction Heater at 39.6C
@14:06:49.50 ATV.dial ASV
@14:06:50.39 ASV.dial :SPEdelivery
@14:06:50.48 AS.seek 0.042ml
@14:08:16.84 delay 10 seconds
@14:08:26.88 Rinsing external lysate line extSrc
@14:08:26.89 AFV.select :carrierSolution
@14:08:27.01 delay 2 seconds
@14:08:29.03 AS.reconfigure ASconfig
@14:08:29.09 AS.seek 1ml
@14:09:24.47 delay 1 second
@14:09:25.49 AFV.select :holdingCoil
@14:09:25.60 delay 1 second
@14:09:26.62 ATV.dial :extSrc
@14:09:27.45 AS.reconfigure ASnorm
@14:09:27.50 AS.seek 0ml
@14:10:18.19 ATV.dial :air2
@14:10:19.80 AS.reconfigure ASslow0
@14:10:19.86 AS.seek 0.05ml
@14:10:25.28 ATV.dial :extSrc
@14:10:26.86 AS.seek 0.01ml
@14:10:31.20 ATV.dial :waste
@14:10:32.57 AS.reconfigure ASconfig
@14:10:32.62 AS.seek 0ml
@14:10:33.84 ethanol wash
@14:10:33.85 ATV.dial :air2
@14:10:35.03 AS.reconfigure ASslow2
@14:10:35.09 AS.seek 0.01ml
@14:10:45.97 delay 3 seconds
@14:10:49.00 Pulling 0.4ml of Ethanol Wash
@14:10:49.01 ATV.dial :ethanol
@14:10:49.89 AS.reconfigure ASslow1
@14:10:49.95 AS.seek 0.41ml
@14:12:10.64 delay 3 seconds
@14:12:13.67 ATV.dial :air1
@14:12:14.09 delay 3 seconds
@14:12:17.16 AS.seek 0.435ml
@14:12:22.46 delay 3 seconds
@14:12:25.49 ATV.dial :ethanol
@14:12:26.12 AS.seek 0.42ml
@14:12:29.42 delay 3 seconds
@14:12:32.45 ATV.dial ASV
@14:12:33.84 AS.seek 0.093ml
@14:13:39.92 delay 3 seconds
@14:13:42.95 ATV.dial :waste2
@14:13:43.75 AS.reconfigure ASconfig
@14:13:43.80 AS.seek 0ml
@14:13:49.45 ATV.dial :air2
@14:13:49.87 AS.reconfigure ASslow1
@14:13:49.92 AS.seek 1ml
@14:17:11.38 delay 5 seconds
@14:17:16.41 ATV.dial ASV
@14:17:17.45 ASV.dial :SPEdelivery
@14:17:17.48 AS.seek 0.02ml
@14:20:34.80 delay 5 seconds
@14:20:39.83 ATV.dial :air2
@14:20:40.92 AS.seek 0.52ml
@14:22:21.75 delay 5 seconds
@14:22:26.78 ATV.dial ASV
@14:22:27.83 ASV.dial :wasteR
@14:22:28.21 AS.seek 0.3ml
@14:23:12.73 delay 20 seconds
@14:23:32.77 AS.seek 0.07ml
@14:24:19.41 delay 20 seconds
@14:24:39.44 ATV.dial :waste2
@14:24:40.25 AS.reconfigure ASconfig
@14:24:40.30 AS.seek 0ml
@14:24:44.64 Elution
@14:24:44.66 ATV.dial :air1
@14:24:45.86 AS.reconfigure ASslow1
@14:24:45.91 AS.seek 0.05ml
@14:24:56.25 delay 3 seconds
@14:24:59.28 ATV.dial :ElutionWater
@14:25:00.00 AS.seek 0.11ml
@14:25:12.31 delay 3 seconds
@14:25:15.34 ATV.dial ASV,via: :air2
@14:25:17.56 ASV.dial :SPEelution
@14:25:18.17 AFV.select :carrierSolution
@14:25:18.33 AS.reconfigure ASnorm
@14:25:18.39 AS.seek 0.76ml
@14:25:51.44 delay 3 seconds
@14:25:54.46 AFV.select :holdingCoil
@14:25:54.61 AS.reconfigure ASslow1
@14:25:54.67 AS.seek 0.475ml
@14:26:52.27 delay 5 seconds
@14:26:57.35 AS.reconfigure ASslow2
@14:26:57.41 AS.seek 0.44ml
@14:27:32.77 delay 1:00
@14:28:32.81 pushing eluate to holding coil
@14:28:32.88 AS.seek 0.28ml
@14:31:14.47 delay 30 seconds
@14:31:44.51 ATV.dial :waste,via: :air3
@14:31:46.35 AS.reconfigure ASconfig
@14:31:46.40 AS.seek 0ml
@14:32:02.78 Parking Bleach in LysateSource line and SPEcolumn
@14:32:02.79 ATV.dial :air3
@14:32:03.70 AS.reconfigure ASslow1
@14:32:03.75 AS.seek 0.03ml
@14:32:10.06 ATV.dial :ElutionWater
@14:32:10.70 AS.seek 0.06ml
@14:32:17.00 ATV.dial :air3
@14:32:17.65 AS.seek 0.07ml
@14:32:19.92 delay 2 seconds
@14:32:21.95 ATV.dial :bleach
@14:32:22.42 AS.seek 0.15ml
@14:32:38.69 delay 3 seconds
@14:32:41.72 ATV.dial :air3
@14:32:42.30 delay 3 seconds
@14:32:45.37 AS.seek 0.175ml
@14:32:50.66 delay 3 seconds
@14:32:53.69 ATV.dial :bleach
@14:32:54.33 AS.seek 0.16ml
@14:32:57.66 delay 3 seconds
@14:33:00.69 ATV.dial :extSrc
@14:33:01.34 AS.reconfigure ASconfig
@14:33:01.39 AS.seek 0.015ml
@14:33:10.02 ATV.dial :waste
@14:33:11.56 AS.seek 0ml
@14:33:12.70 ATV.dial :air3
@14:33:13.58 AS.reconfigure ASslow1
@14:33:13.63 AS.seek 0.01ml
@14:33:16.05 delay 2 seconds
@14:33:18.08 ATV.dial :bleach
@14:33:18.55 AS.seek 0.58ml
@14:35:13.47 delay 3 seconds
@14:35:16.50 ATV.dial :air3
@14:35:17.08 delay 3 seconds
@14:35:20.15 AS.seek 0.605ml
@14:35:25.51 delay 3 seconds
@14:35:28.54 ATV.dial :bleach
@14:35:29.19 AS.seek 0.59ml
@14:35:32.48 delay 3 seconds
@14:35:35.51 ATV.dial :ASV
@14:35:36.59 ASV.dial :wasteR
@14:35:37.21 AS.seek 0ml
@14:37:36.11 delay 1:00
@14:38:36.14 AFV.select :carrierSolution
@14:38:36.25 delay 2 seconds
@14:38:38.27 AS.reconfigure ASconfig
@14:38:38.32 AS.seek 1ml
@14:39:36.09 delay 1 second
@14:39:37.11 AFV.select :holdingCoil
@14:39:37.22 delay 1 second
@14:39:38.24 ATV.dial ASV
@14:39:38.25 ASV.dial :wasteR
@14:39:38.27 AS.reconfigure ASslow1
@14:39:38.34 AS.seek 0ml
@14:42:59.72 delay 20 seconds
@14:43:19.76 ATV.dial :air3
@14:43:21.36 AS.seek 0.59ml
@14:45:20.30 delay 3 seconds
@14:45:23.33 ATV.dial :ASV
@14:45:24.84 ASV.dial :wasteR
@14:45:24.93 AS.seek 0.09ml
@14:47:05.78 delay 30 seconds
@14:47:35.82 ATV.dial :extSrc
@14:47:36.73 delay 3 seconds
@14:47:39.76 ATV.dial :ElutionWater
@14:47:40.96 AS.seek 0.12ml
@14:47:47.28 ATV.dial :air3
@14:47:47.94 AS.seek 0.13ml
@14:47:50.27 delay 2 seconds
@14:47:52.30 ATV.dial :bleach
@14:47:52.79 AS.seek 0.23ml
@14:48:13.22 delay 3 seconds
@14:48:16.25 ATV.dial :air3
@14:48:16.84 delay 3 seconds
@14:48:19.91 AS.seek 0.255ml
@14:48:25.22 delay 3 seconds
@14:48:28.25 ATV.dial :bleach
@14:48:28.89 AS.seek 0.24ml
@14:48:32.63 delay 3 seconds
@14:48:35.66 ATV.dial :ASV
@14:48:36.75 ASV.dial :SPEdelivery
@14:48:37.16 AFV.select :carrierSolution
@14:48:37.28 delay 2 seconds
@14:48:39.30 AS.reconfigure ASconfig
@14:48:39.35 AS.seek 0.5ml
@14:48:54.61 delay 1 second
@14:48:55.63 AFV.select :holdingCoil
@14:48:55.78 AS.reconfigure ASslow1
@14:48:55.84 AS.seek 0.17ml
@14:50:02.53 SPE.hold 2:46:40,95C
@14:50:02.59 delay 3:00
@14:53:02.63 AFV.select :carrierSolution
@14:53:02.75 delay 2 seconds
@14:53:04.77 AS.reconfigure ASconfig
@14:53:04.82 AS.seek 1ml
@14:53:52.81 delay 1 second
@14:53:53.83 AFV.select :holdingCoil
@14:53:53.94 delay 1 second
@14:53:54.96 ASV.dial :wasteD
@14:53:56.09 AS.seek 0ml
@14:54:53.84 Priming Eluate
@14:54:53.85 AFV.select :carrierSolution
@14:54:53.98 delay 2 seconds
@14:54:56.00 AS.seek 0.5ml
@14:55:25.05 delay 1 second
@14:55:26.07 AFV.select :holdingCoil
@14:55:26.18 ATV.dial ASV
@14:55:26.19 ASV.dial :SPErecovery
@14:55:26.83 Swish #1 of 2
@14:55:26.91 AS.reconfigure ASslow0
@14:55:26.97 AS.seek 0.6ml
@14:55:37.44 AS.seek 0.5ml
@14:55:47.86 Swish #2 of 2
@14:55:47.95 AS.seek 0.6ml
@14:55:58.44 AS.seek 0.5ml
@14:56:08.83 delay 3 seconds
@14:56:11.90 AS.reconfigure ASslow1
@14:56:11.96 AS.seek 0.684ml
@14:56:49.27 delay 3 seconds
@14:56:52.30 Eluate primed
@14:56:52.31 ATV.dial :waste2
@14:56:52.98 AS.reconfigure ASnorm
@14:56:53.03 AS.seek 0ml
@14:57:27.80 SPE.coast
@14:57:27.88 ASV.dial :SPErecovery
@14:57:27.90 Setting up PCR for ACYPP2 -- writing /var/log/esp/ACYPP221apr0614h.pcr
@14:57:27.92 Building PCR Slug from ASV with MM1 mix, PP2 primer
@14:57:27.95 Priming with carrier
@14:57:27.97 ATV.dial ARV
@14:57:29.00 ARV.dial :carrier
@14:57:29.36 AS.seek 0.5ml
@14:57:54.95 AFV.select :carrierSolution
@14:57:55.08 delay 2 seconds
@14:57:57.10 AS.reconfigure ASconfig
@14:57:57.17 AS.seek 1ml
@14:58:26.20 delay 1 second
@14:58:27.22 AFV.select :holdingCoil
@14:58:27.33 delay 1 second
@14:58:28.35 ATV.dial :waste
@14:58:29.95 AS.reconfigure ASnorm
@14:58:30.01 AS.seek 0ml
@14:59:20.70 AFV.select :carrierSolution
@14:59:20.83 delay 2 seconds
@14:59:22.85 AS.reconfigure ASconfig
@14:59:22.91 AS.seek 0.5ml
@14:59:51.94 delay 1 second
@14:59:52.96 AFV.select :holdingCoil
@14:59:53.07 Building Slug
@14:59:53.08 ATV.dial :ARV
@14:59:54.83 delay 2 seconds
@14:59:56.86 ARV.dial :air1
@14:59:57.25 delay 2 seconds
@14:59:59.33 AS.reconfigure ASslow2
@14:59:59.39 AS.seek 0.506ml
@15:00:05.86 delay 2 seconds
@15:00:07.89 ARV.dial :MM1
@15:00:08.52 delay 2 seconds
@15:00:10.55 Adding MM1
@15:00:10.62 AS.seek 0.524ml
@15:00:29.19 delay 25 seconds
@15:00:54.22 ARV.dial :PP2
@15:00:54.64 Adding PP2
@15:00:54.65 delay 2 seconds
@15:00:56.73 AS.seek 0.53ml
@15:01:03.11 delay 10 seconds
@15:01:13.15 ARV.dial :air2
@15:01:13.57 adding ARV air
@15:01:13.58 delay 2 seconds
@15:01:15.66 AS.seek 0.536ml
@15:01:21.97 delay 5 seconds
@15:01:27.00 ARV.dial :carrier
@15:01:28.34 delay 7 seconds
@15:01:35.42 AS.seek 0.594ml
@15:02:33.88 delay 5 seconds
@15:02:38.91 ATV.dial :ASV
@15:02:39.34 delay 2 seconds
@15:02:41.42 AS.seek 0.6ml
@15:02:48.00 delay 4 seconds
@15:02:52.03 ATV.dial ARV
@15:02:52.70 delay 2 seconds
@15:02:54.73 ARV.dial :carrier
@15:02:54.74 delay 2 seconds
@15:02:56.82 AS.seek 0.65ml
@15:03:47.78 delay 4 seconds
@15:03:51.81 ATV.dial PCR
@15:03:52.44 ASV.dial :waste1
@15:03:53.27 ARV.dial :air1
@15:03:53.70 Positioning Slug by 0.324ml
@15:03:53.78 AS.seek 0.326ml
@15:09:19.86 delay 5 seconds
@15:09:24.89 ATV.dial ARV
@15:09:25.69 ASV.dial :PCRlock
@15:09:26.08 delay 5 seconds
@15:09:31.11 Running ACYPP2 Profile60CIQ2 thermocycling
@15:09:31.12 <PCR> PCR.program Profile60CIQ2
@15:09:31.24 Profile60CIQ2 should complete in < 1:47:50
@15:09:31.26 PCR.run 2:19:47.5
@16:38:23.56 PCR.off
@16:38:24.20 <MFBPCR> Running ACYPP2 MeltProfile60 thermocycling
@16:38:24.21 <PCR> PCR.program MeltProfile60
@16:38:24.32 MeltProfile60 should complete in < 11:55
@16:38:24.33 PCR.run 19:53.75
@16:46:08.30 PCR.off
@16:46:09.25 <MFBPCR> PCR for ACYPP2 completed -- closed /var/log/esp/ACYPP221apr0614h.pcr
@16:46:09.33 Cleaning PCR
@16:46:09.34 AFV.select :carrierSolution
@16:46:09.46 delay 2 seconds
@16:46:11.48 AS.reconfigure ASconfig
@16:46:11.54 AS.seek 0.5ml
@16:46:21.86 delay 1 second
@16:46:22.88 AFV.select :holdingCoil
@16:46:22.99 ASV.dial :waste1
@16:46:23.39 ATV.dial :air3
@16:46:24.73 AS.reconfigure ASslow1
@16:46:24.79 AS.seek 0.51ml
@16:46:27.06 delay 2 seconds
@16:46:29.09 ATV.dial :bleach
@16:46:29.73 AS.seek 0.81ml
@16:47:30.31 delay 3 seconds
@16:47:33.34 ATV.dial :air3
@16:47:33.92 delay 3 seconds
@16:47:36.99 AS.seek 0.835ml
@16:47:42.42 delay 3 seconds
@16:47:45.45 ATV.dial :bleach
@16:47:46.09 AS.seek 0.82ml
@16:47:49.34 delay 3 seconds
@16:47:52.37 ARV.dial :waste
@16:47:53.01 delay 3 seconds
@16:47:56.04 ATV.dial :ARV
@16:47:56.97 AS.reconfigure ASconfig
@16:47:57.03 AS.seek 0.47ml
@16:48:17.42 AFV.select :carrierSolution
@16:48:17.54 delay 2 seconds
@16:48:19.56 AS.seek 1ml
@16:48:50.31 delay 1 second
@16:48:51.33 AFV.select :holdingCoil
@16:48:51.44 delay 1 second
@16:48:52.46 AS.reconfigure ASfast
@16:48:52.52 AS.seek 0ml
@16:49:19.83 AFV.select :carrierSolution
@16:49:19.95 delay 2 seconds
@16:49:21.97 AS.reconfigure ASconfig
@16:49:22.03 AS.seek 1ml
@16:50:19.78 delay 1 second
@16:50:20.80 AFV.select :holdingCoil
@16:50:20.91 delay 1 second
@16:50:21.93 AS.reconfigure ASfast
@16:50:21.99 AS.seek 0ml
@16:50:49.31 AFV.select :carrierSolution
@16:50:49.43 delay 2 seconds
@16:50:51.45 AS.reconfigure ASconfig
@16:50:51.50 AS.seek 1ml
@16:51:49.25 delay 1 second
@16:51:50.27 AFV.select :holdingCoil
@16:51:50.38 delay 1 second
@16:51:51.40 AS.reconfigure ASfast
@16:51:51.46 AS.seek 0ml
@16:52:18.76 ARV.dial between carrier and air1
@16:52:19.28 ATV.dial :air3
@16:52:20.64 AS.reconfigure ASslow1
@16:52:20.70 AS.seek 0.01ml
@16:52:23.03 delay 2 seconds
@16:52:25.06 ATV.dial :bleach
@16:52:25.70 AS.seek 0.81ml
@16:55:06.86 delay 3 seconds
@16:55:09.89 ATV.dial :air3
@16:55:10.47 delay 3 seconds
@16:55:13.54 AS.seek 0.835ml
@16:55:18.85 delay 3 seconds
@16:55:21.88 ATV.dial :bleach
@16:55:22.51 AS.seek 0.82ml
@16:55:25.81 delay 3 seconds
@16:55:28.84 ATV.dial PCR
@16:55:29.26 AS.reconfigure ASconfig
@16:55:29.31 AS.seek 0ml
@16:56:16.75 PCR.program :bleachProfile
@16:56:16.82 PCR program should complete in < 2:45
@16:56:16.83 PCR.run 10:00
@16:59:13.03 PCR.off
@16:59:13.08 AFV.select :carrierSolution
@16:59:13.19 delay 2 seconds
@16:59:15.21 AS.seek 1ml
@17:00:12.98 delay 1 second
@17:00:14.00 AFV.select :holdingCoil
@17:00:14.11 delay 1 second
@17:00:15.13 AS.reconfigure ASfast
@17:00:15.19 AS.seek 0ml
@17:00:42.53 delay 3 seconds
@17:00:45.55 AFV.select :carrierSolution
@17:00:45.66 delay 2 seconds
@17:00:47.68 AS.reconfigure ASconfig
@17:00:47.74 AS.seek 1ml
@17:01:45.53 delay 1 second
@17:01:46.55 AFV.select :holdingCoil
@17:01:46.66 delay 1 second
@17:01:47.68 AS.reconfigure ASfast
@17:01:47.74 AS.seek 0ml
@17:02:15.08 delay 3 seconds
@17:02:18.10 AFV.select :carrierSolution
@17:02:18.21 delay 2 seconds
@17:02:20.23 AS.reconfigure ASconfig
@17:02:20.30 AS.seek 1ml
@17:03:18.05 delay 1 second
@17:03:19.07 AFV.select :holdingCoil
@17:03:19.18 delay 1 second
@17:03:20.20 AS.reconfigure ASfast
@17:03:20.26 AS.seek 0ml
@17:03:47.58 delay 3 seconds
@17:03:50.60 AFV.select :carrierSolution
@17:03:50.71 delay 2 seconds
@17:03:52.73 AS.reconfigure ASconfig
@17:03:52.80 AS.seek 1ml
@17:04:50.55 delay 1 second
@17:04:51.57 AFV.select :holdingCoil
@17:04:51.68 delay 1 second
@17:04:52.70 AS.reconfigure ASfast
@17:04:52.76 AS.seek 0ml
@17:05:20.11 delay 3 seconds
@17:05:23.13 AFV.select :carrierSolution
@17:05:23.24 delay 2 seconds
@17:05:25.26 AS.reconfigure ASconfig
@17:05:25.32 AS.seek 1ml
@17:06:23.08 delay 1 second
@17:06:24.10 AFV.select :holdingCoil
@17:06:24.21 delay 1 second
@17:06:25.23 AS.reconfigure ASnorm
@17:06:25.29 AS.seek 0ml
@17:07:16.06 Setting up PCR for AOPP1 -- writing /var/log/esp/AOPP121apr0617h.pcr
@17:07:16.07 Building PCR Slug from ASV with MM1 mix, PP1 primer
@17:07:16.09 Priming with carrier
@17:07:16.11 ATV.dial ARV
@17:07:16.76 ARV.dial :carrier
@17:07:17.11 AS.seek 0.5ml
@17:07:42.65 AFV.select :carrierSolution
@17:07:42.78 delay 2 seconds
@17:07:44.80 AS.reconfigure ASconfig
@17:07:44.87 AS.seek 1ml
@17:08:13.87 delay 1 second
@17:08:14.89 AFV.select :holdingCoil
@17:08:15.00 delay 1 second
@17:08:16.02 ATV.dial :waste
@17:08:17.79 AS.reconfigure ASnorm
@17:08:17.85 AS.seek 0ml
@17:09:08.56 AFV.select :carrierSolution
@17:09:08.68 delay 2 seconds
@17:09:10.70 AS.reconfigure ASconfig
@17:09:10.76 AS.seek 0.5ml
@17:09:39.80 delay 1 second
@17:09:40.82 AFV.select :holdingCoil
@17:09:40.93 Building Slug
@17:09:40.94 ATV.dial :ARV
@17:09:42.67 delay 2 seconds
@17:09:44.70 ARV.dial :air1
@17:09:45.09 delay 2 seconds
@17:09:47.16 AS.reconfigure ASslow2
@17:09:47.22 AS.seek 0.506ml
@17:09:53.67 delay 2 seconds
@17:09:55.70 ARV.dial :MM1
@17:09:56.33 delay 2 seconds
@17:09:58.36 Adding MM1
@17:09:58.43 AS.seek 0.524ml
@17:10:17.08 delay 25 seconds
@17:10:42.11 ARV.dial :PP1
@17:10:42.51 Adding PP1
@17:10:42.52 delay 2 seconds
@17:10:44.60 AS.seek 0.53ml
@17:10:50.78 delay 10 seconds
@17:11:00.82 ARV.dial :air1
@17:11:01.24 adding ARV air
@17:11:01.25 delay 2 seconds
@17:11:03.33 AS.seek 0.536ml
@17:11:09.73 delay 5 seconds
@17:11:14.76 ARV.dial :carrier
@17:11:15.19 delay 7 seconds
@17:11:22.27 AS.seek 0.594ml
@17:12:21.15 delay 5 seconds
@17:12:26.18 ATV.dial :ASV
@17:12:26.61 delay 2 seconds
@17:12:28.69 AS.seek 0.6ml
@17:12:35.14 delay 4 seconds
@17:12:39.17 ATV.dial ARV
@17:12:39.84 delay 2 seconds
@17:12:41.87 ARV.dial :carrier
@17:12:41.88 delay 2 seconds
@17:12:43.96 AS.seek 0.65ml
@17:13:35.59 delay 4 seconds
@17:13:39.62 ATV.dial PCR
@17:13:40.25 ASV.dial :waste1
@17:13:40.27 ARV.dial :air1
@17:13:40.71 Positioning Slug by 0.324ml
@17:13:40.79 AS.seek 0.326ml
@17:19:06.81 delay 5 seconds
@17:19:11.84 ATV.dial ARV
@17:19:12.64 ASV.dial :PCRlock
@17:19:13.05 delay 5 seconds
@17:19:18.08 Running AOPP1 Profile60CIQ1 thermocycling
@17:19:18.09 <PCR> PCR.program Profile60CIQ1
@17:19:18.21 Profile60CIQ1 should complete in < 1:56:15
@17:19:18.22 PCR.run 2:30:18.75
@18:58:38.39 PCR.off
@18:58:39.34 <MFBPCR> Running AOPP1 MeltProfile60 thermocycling
@18:58:39.35 <PCR> PCR.program MeltProfile60
@18:58:39.46 MeltProfile60 should complete in < 11:55
@18:58:39.47 PCR.run 19:53.75
@19:06:17.62 PCR.off
@19:06:18.57 <MFBPCR> PCR for AOPP1 completed -- closed /var/log/esp/AOPP121apr0617h.pcr
@19:06:18.64 Cleaning PCR
@19:06:18.65 AFV.select :carrierSolution
@19:06:18.77 delay 2 seconds
@19:06:20.79 AS.reconfigure ASconfig
@19:06:20.84 AS.seek 0.5ml
@19:06:31.14 delay 1 second
@19:06:32.16 AFV.select :holdingCoil
@19:06:32.27 ASV.dial :waste1
@19:06:32.67 ATV.dial :air3
@19:06:34.01 AS.reconfigure ASslow1
@19:06:34.07 AS.seek 0.51ml
@19:06:36.34 delay 2 seconds
@19:06:38.37 ATV.dial :bleach
@19:06:39.01 AS.seek 0.81ml
@19:07:39.69 delay 3 seconds
@19:07:42.72 ATV.dial :air3
@19:07:43.29 delay 3 seconds
@19:07:46.36 AS.seek 0.835ml
@19:07:51.68 delay 3 seconds
@19:07:54.71 ATV.dial :bleach
@19:07:55.35 AS.seek 0.82ml
@19:07:58.69 delay 3 seconds
@19:08:01.74 ARV.dial :waste
@19:08:02.39 delay 3 seconds
@19:08:05.42 ATV.dial :ARV
@19:08:06.34 AS.reconfigure ASconfig
@19:08:06.39 AS.seek 0.47ml
@19:08:26.81 AFV.select :carrierSolution
@19:08:26.93 delay 2 seconds
@19:08:28.95 AS.seek 1ml
@19:08:59.73 delay 1 second
@19:09:00.75 AFV.select :holdingCoil
@19:09:00.86 delay 1 second
@19:09:01.88 AS.reconfigure ASfast
@19:09:01.94 AS.seek 0ml
@19:09:29.29 AFV.select :carrierSolution
@19:09:29.41 delay 2 seconds
@19:09:31.43 AS.reconfigure ASconfig
@19:09:31.48 AS.seek 1ml
@19:10:29.22 delay 1 second
@19:10:30.24 AFV.select :holdingCoil
@19:10:30.35 delay 1 second
@19:10:31.37 AS.reconfigure ASfast
@19:10:31.43 AS.seek 0ml
@19:10:58.78 AFV.select :carrierSolution
@19:10:58.90 delay 2 seconds
@19:11:00.92 AS.reconfigure ASconfig
@19:11:00.98 AS.seek 1ml
@19:11:58.73 delay 1 second
@19:11:59.75 AFV.select :holdingCoil
@19:11:59.86 delay 1 second
@19:12:00.88 AS.reconfigure ASfast
@19:12:00.94 AS.seek 0ml
@19:12:28.29 ARV.dial between carrier and air1
@19:12:28.81 ATV.dial :air3
@19:12:30.15 AS.reconfigure ASslow1
@19:12:30.21 AS.seek 0.01ml
@19:12:32.59 delay 2 seconds
@19:12:34.62 ATV.dial :bleach
@19:12:35.26 AS.seek 0.81ml
@19:15:16.40 delay 3 seconds
@19:15:19.43 ATV.dial :air3
@19:15:20.01 delay 3 seconds
@19:15:23.08 AS.seek 0.835ml
@19:15:28.38 delay 3 seconds
@19:15:31.41 ATV.dial :bleach
@19:15:32.04 AS.seek 0.82ml
@19:15:35.34 delay 3 seconds
@19:15:38.37 ATV.dial PCR
@19:15:38.80 AS.reconfigure ASconfig
@19:15:38.85 AS.seek 0ml
@19:16:26.26 PCR.program :bleachProfile
@19:16:26.33 PCR program should complete in < 2:45
@19:16:26.34 PCR.run 10:00
@19:19:22.54 PCR.off
@19:19:22.59 AFV.select :carrierSolution
@19:19:22.70 delay 2 seconds
@19:19:24.72 AS.seek 1ml
@19:20:22.47 delay 1 second
@19:20:23.49 AFV.select :holdingCoil
@19:20:23.60 delay 1 second
@19:20:24.62 AS.reconfigure ASfast
@19:20:24.68 AS.seek 0ml
@19:20:52.01 delay 3 seconds
@19:20:55.03 AFV.select :carrierSolution
@19:20:55.14 delay 2 seconds
@19:20:57.16 AS.reconfigure ASconfig
@19:20:57.22 AS.seek 1ml
@19:21:54.97 delay 1 second
@19:21:55.99 AFV.select :holdingCoil
@19:21:56.10 delay 1 second
@19:21:57.12 AS.reconfigure ASfast
@19:21:57.18 AS.seek 0ml
@19:22:24.53 delay 3 seconds
@19:22:27.55 AFV.select :carrierSolution
@19:22:27.66 delay 2 seconds
@19:22:29.68 AS.reconfigure ASconfig
@19:22:29.75 AS.seek 1ml
@19:23:27.48 delay 1 second
@19:23:28.50 AFV.select :holdingCoil
@19:23:28.61 delay 1 second
@19:23:29.63 AS.reconfigure ASfast
@19:23:29.69 AS.seek 0ml
@19:23:57.03 delay 3 seconds
@19:24:00.05 AFV.select :carrierSolution
@19:24:00.16 delay 2 seconds
@19:24:02.18 AS.reconfigure ASconfig
@19:24:02.25 AS.seek 1ml
@19:25:00.00 delay 1 second
@19:25:01.02 AFV.select :holdingCoil
@19:25:01.13 delay 1 second
@19:25:02.15 AS.reconfigure ASfast
@19:25:02.21 AS.seek 0ml
@19:25:29.56 delay 3 seconds
@19:25:32.58 AFV.select :carrierSolution
@19:25:32.69 delay 2 seconds
@19:25:34.71 AS.reconfigure ASconfig
@19:25:34.77 AS.seek 1ml
@19:26:32.51 delay 1 second
@19:26:33.53 AFV.select :holdingCoil
@19:26:33.64 delay 1 second
@19:26:34.66 AS.reconfigure ASnorm
@19:26:34.72 AS.seek 0ml
@19:27:25.40 Setting up PCR for AARPP4 -- writing /var/log/esp/AARPP421apr0619h.pcr
@19:27:25.41 Building PCR Slug from ASV with MM2 mix, PP4 primer
@19:27:25.44 Priming with carrier
@19:27:25.45 ATV.dial ARV
@19:27:26.09 ARV.dial :carrier
@19:27:26.45 AS.seek 0.5ml
@19:27:51.98 AFV.select :carrierSolution
@19:27:52.10 delay 2 seconds
@19:27:54.12 AS.reconfigure ASconfig
@19:27:54.19 AS.seek 1ml
@19:28:23.22 delay 1 second
@19:28:24.24 AFV.select :holdingCoil
@19:28:24.35 delay 1 second
@19:28:25.37 ATV.dial :waste
@19:28:27.12 AS.reconfigure ASnorm
@19:28:27.18 AS.seek 0ml
@19:29:17.86 AFV.select :carrierSolution
@19:29:17.98 delay 2 seconds
@19:29:20.00 AS.reconfigure ASconfig
@19:29:20.07 AS.seek 0.5ml
@19:29:49.14 delay 1 second
@19:29:50.16 AFV.select :holdingCoil
@19:29:50.27 Building Slug
@19:29:50.28 ATV.dial :ARV
@19:29:52.03 delay 2 seconds
@19:29:54.06 ARV.dial :air1
@19:29:54.46 delay 2 seconds
@19:29:56.53 AS.reconfigure ASslow2
@19:29:56.59 AS.seek 0.506ml
@19:30:03.47 delay 2 seconds
@19:30:05.49 ARV.dial :MM2
@19:30:07.08 delay 2 seconds
@19:30:09.11 Adding MM2
@19:30:09.18 AS.seek 0.524ml
@19:30:27.83 delay 25 seconds
@19:30:52.86 ARV.dial :PP4
@19:30:53.29 Adding PP4
@19:30:53.30 delay 2 seconds
@19:30:55.37 AS.seek 0.53ml
@19:31:02.04 delay 10 seconds
@19:31:12.08 ARV.dial :air3
@19:31:12.49 adding ARV air
@19:31:12.50 delay 2 seconds
@19:31:14.57 AS.seek 0.536ml
@19:31:20.72 delay 5 seconds
@19:31:25.75 ARV.dial :carrier
@19:31:27.08 delay 7 seconds
@19:31:34.17 AS.seek 0.594ml
@19:32:32.78 delay 5 seconds
@19:32:37.81 ATV.dial :ASV
@19:32:38.23 delay 2 seconds
@19:32:40.31 AS.seek 0.6ml
@19:32:46.73 delay 4 seconds
@19:32:50.76 ATV.dial ARV
@19:32:51.33 delay 2 seconds
@19:32:53.36 ARV.dial :carrier
@19:32:53.37 delay 2 seconds
@19:32:55.44 AS.seek 0.65ml
@19:33:46.39 delay 4 seconds
@19:33:50.42 ATV.dial PCR
@19:33:51.06 ASV.dial :waste1
@19:33:51.08 ARV.dial :air1
@19:33:51.50 Positioning Slug by 0.324ml
@19:33:51.58 AS.seek 0.326ml
@19:39:17.65 delay 5 seconds
@19:39:22.68 ATV.dial ARV
@19:39:23.48 ASV.dial :PCRlock
@19:39:23.89 delay 5 seconds
@19:39:28.92 Running AARPP4 Profile58CIQ2 thermocycling
@19:39:28.93 <PCR> PCR.program Profile58CIQ2
@19:39:29.05 Profile58CIQ2 should complete in < 1:36:15
@19:39:29.06 PCR.run 2:05:18.75
@21:00:26.24 PCR.off
@21:00:26.81 <MFBPCR> Running AARPP4 MeltProfile58 thermocycling
@21:00:26.82 <PCR> PCR.program MeltProfile58
@21:00:26.93 MeltProfile58 should complete in < 12:33
@21:00:26.94 PCR.run 20:41.25
@21:08:08.67 PCR.off
@21:08:09.67 <MFBPCR> PCR for AARPP4 completed -- closed /var/log/esp/AARPP421apr0619h.pcr
@21:08:09.75 Cleaning PCR
@21:08:09.76 AFV.select :carrierSolution
@21:08:09.88 delay 2 seconds
@21:08:11.90 AS.reconfigure ASconfig
@21:08:11.96 AS.seek 0.5ml
@21:08:22.31 delay 1 second
@21:08:23.33 AFV.select :holdingCoil
@21:08:23.44 ASV.dial :waste1
@21:08:23.83 ATV.dial :air3
@21:08:25.18 AS.reconfigure ASslow1
@21:08:25.24 AS.seek 0.51ml
@21:08:27.73 delay 2 seconds
@21:08:29.76 ATV.dial :bleach
@21:08:30.40 AS.seek 0.81ml
@21:09:31.09 delay 3 seconds
@21:09:34.12 ATV.dial :air3
@21:09:34.70 delay 3 seconds
@21:09:37.77 AS.seek 0.835ml
@21:09:43.20 delay 3 seconds
@21:09:46.23 ATV.dial :bleach
@21:09:46.87 AS.seek 0.82ml
@21:09:50.17 delay 3 seconds
@21:09:53.20 ARV.dial :waste
@21:09:53.83 delay 3 seconds
@21:09:56.86 ATV.dial :ARV
@21:09:57.78 AS.reconfigure ASconfig
@21:09:57.83 AS.seek 0.47ml
@21:10:18.23 AFV.select :carrierSolution
@21:10:18.35 delay 2 seconds
@21:10:20.37 AS.seek 1ml
@21:10:51.14 delay 1 second
@21:10:52.16 AFV.select :holdingCoil
@21:10:52.27 delay 1 second
@21:10:53.29 AS.reconfigure ASfast
@21:10:53.35 AS.seek 0ml
@21:11:20.69 AFV.select :carrierSolution
@21:11:20.81 delay 2 seconds
@21:11:22.83 AS.reconfigure ASconfig
@21:11:22.89 AS.seek 1ml
@21:12:20.64 delay 1 second
@21:12:21.66 AFV.select :holdingCoil
@21:12:21.77 delay 1 second
@21:12:22.79 AS.reconfigure ASfast
@21:12:22.85 AS.seek 0ml
@21:12:50.20 AFV.select :carrierSolution
@21:12:50.32 delay 2 seconds
@21:12:52.75 AS.reconfigure ASconfig
@21:12:52.81 AS.seek 1ml
@21:13:50.55 delay 1 second
@21:13:51.57 AFV.select :holdingCoil
@21:13:51.68 delay 1 second
@21:13:52.70 AS.reconfigure ASfast
@21:13:52.76 AS.seek 0ml
@21:14:20.09 ARV.dial between carrier and air1
@21:14:20.63 ATV.dial :air3
@21:14:21.98 AS.reconfigure ASslow1
@21:14:22.04 AS.seek 0.01ml
@21:14:24.50 delay 2 seconds
@21:14:26.53 ATV.dial :bleach
@21:14:27.17 AS.seek 0.81ml
@21:17:08.44 delay 3 seconds
@21:17:11.47 ATV.dial :air3
@21:17:12.05 delay 3 seconds
@21:17:15.12 AS.seek 0.835ml
@21:17:20.57 delay 3 seconds
@21:17:23.60 ATV.dial :bleach
@21:17:24.25 AS.seek 0.82ml
@21:17:27.56 delay 3 seconds
@21:17:30.59 ATV.dial PCR
@21:17:31.02 AS.reconfigure ASconfig
@21:17:31.07 AS.seek 0ml
@21:18:18.45 PCR.program :bleachProfile
@21:18:18.52 PCR program should complete in < 2:45
@21:18:18.53 PCR.run 10:00
@21:21:14.32 PCR.off
@21:21:14.36 AFV.select :carrierSolution
@21:21:14.47 delay 2 seconds
@21:21:16.49 AS.seek 1ml
@21:22:14.25 delay 1 second
@21:22:15.27 AFV.select :holdingCoil
@21:22:15.38 delay 1 second
@21:22:16.40 AS.reconfigure ASfast
@21:22:16.46 AS.seek 0ml
@21:22:43.80 delay 3 seconds
@21:22:46.82 AFV.select :carrierSolution
@21:22:46.93 delay 2 seconds
@21:22:48.95 AS.reconfigure ASconfig
@21:22:49.01 AS.seek 1ml
@21:23:46.80 delay 1 second
@21:23:47.82 AFV.select :holdingCoil
@21:23:47.93 delay 1 second
@21:23:48.95 AS.reconfigure ASfast
@21:23:49.01 AS.seek 0ml
@21:24:16.36 delay 3 seconds
@21:24:19.38 AFV.select :carrierSolution
@21:24:19.49 delay 2 seconds
@21:24:21.51 AS.reconfigure ASconfig
@21:24:21.58 AS.seek 1ml
@21:25:19.39 delay 1 second
@21:25:20.41 AFV.select :holdingCoil
@21:25:20.52 delay 1 second
@21:25:21.54 AS.reconfigure ASfast
@21:25:21.60 AS.seek 0ml
@21:25:48.94 delay 3 seconds
@21:25:51.96 AFV.select :carrierSolution
@21:25:52.07 delay 2 seconds
@21:25:54.09 AS.reconfigure ASconfig
@21:25:54.14 AS.seek 1ml
@21:26:51.87 delay 1 second
@21:26:52.89 AFV.select :holdingCoil
@21:26:53.00 delay 1 second
@21:26:54.41 AS.reconfigure ASfast
@21:26:54.48 AS.seek 0ml
@21:27:21.83 delay 3 seconds
@21:27:24.85 AFV.select :carrierSolution
@21:27:24.96 delay 2 seconds
@21:27:26.98 AS.reconfigure ASconfig
@21:27:27.04 AS.seek 1ml
@21:28:24.78 delay 1 second
@21:28:25.80 AFV.select :holdingCoil
@21:28:25.91 delay 1 second
@21:28:26.93 AS.reconfigure ASnorm
@21:28:26.99 AS.seek 0ml
@21:29:17.72 Setting up PCR for AALPP3 -- writing /var/log/esp/AALPP321apr0621h.pcr
@21:29:17.74 Building PCR Slug from ASV with MM2 mix, PP3 primer
@21:29:17.76 Priming with carrier
@21:29:17.77 ATV.dial ARV
@21:29:18.42 ARV.dial :carrier
@21:29:18.78 AS.seek 0.5ml
@21:29:44.34 AFV.select :carrierSolution
@21:29:44.47 delay 2 seconds
@21:29:46.49 AS.reconfigure ASconfig
@21:29:46.56 AS.seek 1ml
@21:30:15.59 delay 1 second
@21:30:16.61 AFV.select :holdingCoil
@21:30:16.72 delay 1 second
@21:30:17.74 ATV.dial :waste
@21:30:19.51 AS.reconfigure ASnorm
@21:30:19.57 AS.seek 0ml
@21:31:10.31 AFV.select :carrierSolution
@21:31:10.44 delay 2 seconds
@21:31:12.46 AS.reconfigure ASconfig
@21:31:12.53 AS.seek 0.5ml
@21:31:41.61 delay 1 second
@21:31:42.63 AFV.select :holdingCoil
@21:31:42.74 Building Slug
@21:31:42.75 ATV.dial :ARV
@21:31:44.60 delay 2 seconds
@21:31:46.63 ARV.dial :air1
@21:31:47.03 delay 2 seconds
@21:31:49.10 AS.reconfigure ASslow2
@21:31:49.16 AS.seek 0.506ml
@21:31:55.80 delay 2 seconds
@21:31:57.83 ARV.dial :MM2
@21:31:59.40 delay 2 seconds
@21:32:01.43 Adding MM2
@21:32:01.50 AS.seek 0.524ml
@21:32:20.28 delay 25 seconds
@21:32:45.31 ARV.dial :PP3
@21:32:45.71 Adding PP3
@21:32:45.72 delay 2 seconds
@21:32:47.79 AS.seek 0.53ml
@21:32:54.17 delay 10 seconds
@21:33:04.21 ARV.dial :air2
@21:33:04.63 adding ARV air
@21:33:04.64 delay 2 seconds
@21:33:06.71 AS.seek 0.536ml
@21:33:13.34 delay 5 seconds
@21:33:18.37 ARV.dial :carrier
@21:33:19.75 delay 7 seconds
@21:33:26.83 AS.seek 0.594ml
@21:34:25.30 delay 5 seconds
@21:34:30.33 ATV.dial :ASV
@21:34:30.75 delay 2 seconds
@21:34:32.82 AS.seek 0.6ml
@21:34:39.31 delay 4 seconds
@21:34:43.34 ATV.dial ARV
@21:34:44.01 delay 2 seconds
@21:34:46.04 ARV.dial :carrier
@21:34:46.05 delay 2 seconds
@21:34:48.12 AS.seek 0.65ml
@21:35:39.06 delay 4 seconds
@21:35:43.09 ATV.dial PCR
@21:35:43.73 ASV.dial :waste1
@21:35:43.75 ARV.dial :air1
@21:35:44.16 Positioning Slug by 0.324ml
@21:35:44.25 AS.seek 0.326ml
@21:41:10.92 delay 5 seconds
@21:41:15.95 ATV.dial ARV
@21:41:16.76 ASV.dial :PCRlock
@21:41:17.17 delay 5 seconds
@21:41:22.20 Running AALPP3 Profile58CIQ2 thermocycling
@21:41:22.21 <PCR> PCR.program Profile58CIQ2
@21:41:22.34 Profile58CIQ2 should complete in < 1:36:15
@21:41:22.35 PCR.run 2:05:18.75
@23:02:16.81 PCR.off
@23:02:17.38 <MFBPCR> Running AALPP3 MeltProfile58 thermocycling
@23:02:17.40 <PCR> PCR.program MeltProfile58
@23:02:17.50 MeltProfile58 should complete in < 12:33
@23:02:17.51 PCR.run 20:41.25
@23:09:56.04 PCR.off
@23:09:57.03 <MFBPCR> PCR for AALPP3 completed -- closed /var/log/esp/AALPP321apr0621h.pcr
@23:09:57.10 Cleaning PCR
@23:09:57.11 AFV.select :carrierSolution
@23:09:57.23 delay 2 seconds
@23:09:59.25 AS.reconfigure ASconfig
@23:09:59.31 AS.seek 0.5ml
@23:10:09.61 delay 1 second
@23:10:10.63 AFV.select :holdingCoil
@23:10:11.07 ASV.dial :waste1
@23:10:11.52 ATV.dial :air3
@23:10:12.87 AS.reconfigure ASslow1
@23:10:12.93 AS.seek 0.51ml
@23:10:15.23 delay 2 seconds
@23:10:17.26 ATV.dial :bleach
@23:10:17.90 AS.seek 0.81ml
@23:11:18.48 delay 3 seconds
@23:11:21.51 ATV.dial :air3
@23:11:22.09 delay 3 seconds
@23:11:25.16 AS.seek 0.835ml
@23:11:30.48 delay 3 seconds
@23:11:33.51 ATV.dial :bleach
@23:11:34.15 AS.seek 0.82ml
@23:11:37.48 delay 3 seconds
@23:11:40.51 ARV.dial :waste
@23:11:41.14 delay 3 seconds
@23:11:44.17 ATV.dial :ARV
@23:11:45.09 AS.reconfigure ASconfig
@23:11:45.15 AS.seek 0.47ml
@23:12:05.56 AFV.select :carrierSolution
@23:12:05.68 delay 2 seconds
@23:12:07.70 AS.seek 1ml
@23:12:38.44 delay 1 second
@23:12:39.46 AFV.select :holdingCoil
@23:12:39.57 delay 1 second
@23:12:40.59 AS.reconfigure ASfast
@23:12:40.65 AS.seek 0ml
@23:13:08.00 AFV.select :carrierSolution
@23:13:08.12 delay 2 seconds
@23:13:10.14 AS.reconfigure ASconfig
@23:13:10.20 AS.seek 1ml
@23:14:07.95 delay 1 second
@23:14:08.97 AFV.select :holdingCoil
@23:14:09.08 delay 1 second
@23:14:10.10 AS.reconfigure ASfast
@23:14:10.16 AS.seek 0ml
@23:14:37.50 AFV.select :carrierSolution
@23:14:37.62 delay 2 seconds
@23:14:39.64 AS.reconfigure ASconfig
@23:14:39.70 AS.seek 1ml
@23:15:37.45 delay 1 second
@23:15:38.47 AFV.select :holdingCoil
@23:15:38.58 delay 1 second
@23:15:39.60 AS.reconfigure ASfast
@23:15:39.66 AS.seek 0ml
@23:16:07.00 ARV.dial between carrier and air1
@23:16:07.53 ATV.dial :air3
@23:16:08.89 AS.reconfigure ASslow1
@23:16:08.95 AS.seek 0.01ml
@23:16:11.34 delay 2 seconds
@23:16:13.37 ATV.dial :bleach
@23:16:14.01 AS.seek 0.81ml
@23:18:55.17 delay 3 seconds
@23:18:58.20 ATV.dial :air3
@23:18:58.78 delay 3 seconds
@23:19:01.85 AS.seek 0.835ml
@23:19:07.14 delay 3 seconds
@23:19:10.17 ATV.dial :bleach
@23:19:10.81 AS.seek 0.82ml
@23:19:14.12 delay 3 seconds
@23:19:17.15 ATV.dial PCR
@23:19:17.59 AS.reconfigure ASconfig
@23:19:17.64 AS.seek 0ml
@23:20:05.06 PCR.program :bleachProfile
@23:20:05.13 PCR program should complete in < 2:45
@23:20:05.14 PCR.run 10:00
@23:23:01.35 PCR.off
@23:23:01.40 AFV.select :carrierSolution
@23:23:01.51 delay 2 seconds
@23:23:03.53 AS.seek 1ml
@23:24:01.30 delay 1 second
@23:24:02.32 AFV.select :holdingCoil
@23:24:02.43 delay 1 second
@23:24:03.45 AS.reconfigure ASfast
@23:24:03.51 AS.seek 0ml
@23:24:30.84 delay 3 seconds
@23:24:33.86 AFV.select :carrierSolution
@23:24:33.97 delay 2 seconds
@23:24:35.99 AS.reconfigure ASconfig
@23:24:36.05 AS.seek 1ml
@23:25:33.80 delay 1 second
@23:25:34.82 AFV.select :holdingCoil
@23:25:34.93 delay 1 second
@23:25:35.95 AS.reconfigure ASfast
@23:25:36.01 AS.seek 0ml
@23:26:03.36 delay 3 seconds
@23:26:06.38 AFV.select :carrierSolution
@23:26:06.49 delay 2 seconds
@23:26:08.51 AS.reconfigure ASconfig
@23:26:08.58 AS.seek 1ml
@23:27:06.36 delay 1 second
@23:27:07.38 AFV.select :holdingCoil
@23:27:07.49 delay 1 second
@23:27:08.51 AS.reconfigure ASfast
@23:27:08.57 AS.seek 0ml
@23:27:35.91 delay 3 seconds
@23:27:38.93 AFV.select :carrierSolution
@23:27:39.04 delay 2 seconds
@23:27:41.06 AS.reconfigure ASconfig
@23:27:41.12 AS.seek 1ml
@23:28:38.87 delay 1 second
@23:28:39.88 AFV.select :holdingCoil
@23:28:39.99 delay 1 second
@23:28:41.01 AS.reconfigure ASfast
@23:28:41.08 AS.seek 0ml
@23:29:08.41 delay 3 seconds
@23:29:11.43 AFV.select :carrierSolution
@23:29:11.54 delay 2 seconds
@23:29:13.56 AS.reconfigure ASconfig
@23:29:13.63 AS.seek 1ml
@23:30:11.37 delay 1 second
@23:30:12.39 AFV.select :holdingCoil
@23:30:12.50 delay 1 second
@23:30:13.52 AS.reconfigure ASnorm
@23:30:13.57 AS.seek 0ml
@23:31:04.30 Setting up PCR for ACOPP5 -- writing /var/log/esp/ACOPP521apr0623h.pcr
@23:31:04.31 Building PCR Slug from ASV with MM3 mix, PP5 primer
@23:31:04.34 Priming with carrier
@23:31:04.35 ATV.dial ARV
@23:31:05.00 ARV.dial :carrier
@23:31:05.36 AS.seek 0.5ml
@23:31:30.97 AFV.select :carrierSolution
@23:31:31.09 delay 2 seconds
@23:31:33.11 AS.reconfigure ASconfig
@23:31:33.18 AS.seek 1ml
@23:32:02.19 delay 1 second
@23:32:03.21 AFV.select :holdingCoil
@23:32:03.32 delay 1 second
@23:32:04.34 ATV.dial :waste
@23:32:06.12 AS.reconfigure ASnorm
@23:32:06.18 AS.seek 0ml
@23:32:56.89 AFV.select :carrierSolution
@23:32:57.02 delay 2 seconds
@23:32:59.04 AS.reconfigure ASconfig
@23:32:59.11 AS.seek 0.5ml
@23:33:28.14 delay 1 second
@23:33:29.16 AFV.select :holdingCoil
@23:33:29.27 Building Slug
@23:33:29.28 ATV.dial :ARV
@23:33:31.03 delay 2 seconds
@23:33:33.06 ARV.dial :air1
@23:33:33.46 delay 2 seconds
@23:33:35.53 AS.reconfigure ASslow2
@23:33:35.60 AS.seek 0.506ml
@23:33:42.08 delay 2 seconds
@23:33:44.11 ARV.dial :MM3
@23:33:45.20 delay 2 seconds
@23:33:47.23 Adding MM3
@23:33:47.31 AS.seek 0.524ml
@23:34:05.83 delay 25 seconds
@23:34:30.86 ARV.dial :PP5
@23:34:31.27 Adding PP5
@23:34:31.29 delay 2 seconds
@23:34:33.36 AS.seek 0.53ml
@23:34:40.00 delay 10 seconds
@23:34:50.04 ARV.dial :air3
@23:34:50.44 adding ARV air
@23:34:50.46 delay 2 seconds
@23:34:52.53 AS.seek 0.536ml
@23:34:58.70 delay 5 seconds
@23:35:03.73 ARV.dial :carrier
@23:35:05.06 delay 7 seconds
@23:35:12.14 AS.seek 0.594ml
@23:36:10.61 delay 5 seconds
@23:36:15.64 ATV.dial :ASV
@23:36:16.06 delay 2 seconds
@23:36:18.13 AS.seek 0.6ml
@23:36:24.45 delay 4 seconds
@23:36:28.48 ATV.dial ARV
@23:36:29.06 delay 2 seconds
@23:36:31.09 ARV.dial :carrier
@23:36:31.10 delay 2 seconds
@23:36:33.17 AS.seek 0.65ml
@23:37:23.64 delay 4 seconds
@23:37:27.67 ATV.dial PCR
@23:37:28.31 ASV.dial :waste1
@23:37:28.33 ARV.dial :air1
@23:37:28.74 Positioning Slug by 0.324ml
@23:37:28.83 AS.seek 0.326ml
@23:42:54.99 delay 5 seconds
@23:43:00.01 ATV.dial ARV
@23:43:00.81 ASV.dial :PCRlock
@23:43:01.22 delay 5 seconds
@23:43:06.25 Running ACOPP5 ProfileIPc thermocycling
@23:43:06.26 <PCR> PCR.program ProfileIPc
@23:43:06.38 ProfileIPc should complete in < 1:26:35
@23:43:06.39 PCR.run 1:53:13.75
@00:51:35.22CEST07-Apr-21 PCR.off
@00:51:36.17 <MFBPCR> PCR for ACOPP5 completed -- closed /var/log/esp/ACOPP521apr0623h.pcr
@00:51:36.24 Cleaning PCR
@00:51:36.26 AFV.select :carrierSolution
@00:51:36.38 delay 2 seconds
@00:51:38.40 AS.reconfigure ASconfig
@00:51:38.46 AS.seek 0.5ml
@00:51:48.77 delay 1 second
@00:51:49.79 AFV.select :holdingCoil
@00:51:49.90 ASV.dial :waste1
@00:51:50.28 ATV.dial :air3
@00:51:51.64 AS.reconfigure ASslow1
@00:51:51.70 AS.seek 0.51ml
@00:51:54.06 delay 2 seconds
@00:51:56.09 ATV.dial :bleach
@00:51:56.75 AS.seek 0.81ml
@00:52:57.36 delay 3 seconds
@00:53:00.39 ATV.dial :air3
@00:53:00.97 delay 3 seconds
@00:53:04.04 AS.seek 0.835ml
@00:53:09.36 delay 3 seconds
@00:53:12.39 ATV.dial :bleach
@00:53:13.03 AS.seek 0.82ml
@00:53:16.36 delay 3 seconds
@00:53:19.39 ARV.dial :waste
@00:53:20.02 delay 3 seconds
@00:53:23.05 ATV.dial :ARV
@00:53:23.97 AS.reconfigure ASconfig
@00:53:24.02 AS.seek 0.47ml
@00:53:44.42 AFV.select :carrierSolution
@00:53:44.54 delay 2 seconds
@00:53:46.56 AS.seek 1ml
@00:54:17.30 delay 1 second
@00:54:18.32 AFV.select :holdingCoil
@00:54:18.43 delay 1 second
@00:54:19.45 AS.reconfigure ASfast
@00:54:19.51 AS.seek 0ml
@00:54:46.84 AFV.select :carrierSolution
@00:54:46.96 delay 2 seconds
@00:54:48.98 AS.reconfigure ASconfig
@00:54:49.03 AS.seek 1ml
@00:55:46.78 delay 1 second
@00:55:47.80 AFV.select :holdingCoil
@00:55:47.91 delay 1 second
@00:55:48.93 AS.reconfigure ASfast
@00:55:48.99 AS.seek 0ml
@00:56:16.33 AFV.select :carrierSolution
@00:56:16.45 delay 2 seconds
@00:56:18.47 AS.reconfigure ASconfig
@00:56:18.52 AS.seek 1ml
@00:57:16.25 delay 1 second
@00:57:17.27 AFV.select :holdingCoil
@00:57:17.38 delay 1 second
@00:57:18.40 AS.reconfigure ASfast
@00:57:18.46 AS.seek 0ml
@00:57:45.78 ARV.dial between carrier and air1
@00:57:46.28 ATV.dial :air3
@00:57:47.62 AS.reconfigure ASslow1
@00:57:47.68 AS.seek 0.01ml
@00:57:50.03 delay 2 seconds
@00:57:52.06 ATV.dial :bleach
@00:57:52.70 AS.seek 0.81ml
@01:00:33.92 delay 3 seconds
@01:00:36.95 ATV.dial :air3
@01:00:37.52 delay 3 seconds
@01:00:40.59 AS.seek 0.835ml
@01:00:45.90 delay 3 seconds
@01:00:48.93 ATV.dial :bleach
@01:00:49.56 AS.seek 0.82ml
@01:00:52.81 delay 3 seconds
@01:00:55.84 ATV.dial PCR
@01:00:56.26 AS.reconfigure ASconfig
@01:00:56.31 AS.seek 0ml
@01:01:43.74 PCR.program :bleachProfile
@01:01:43.81 PCR program should complete in < 2:45
@01:01:43.82 PCR.run 10:00
@01:04:40.04 PCR.off
@01:04:40.09 AFV.select :carrierSolution
@01:04:40.20 delay 2 seconds
@01:04:42.22 AS.seek 1ml
@01:05:39.99 delay 1 second
@01:05:41.01 AFV.select :holdingCoil
@01:05:41.12 delay 1 second
@01:05:42.14 AS.reconfigure ASfast
@01:05:42.20 AS.seek 0ml
@01:06:09.53 delay 3 seconds
@01:06:12.55 AFV.select :carrierSolution
@01:06:12.66 delay 2 seconds
@01:06:14.68 AS.reconfigure ASconfig
@01:06:14.74 AS.seek 1ml
@01:07:12.49 delay 1 second
@01:07:13.51 AFV.select :holdingCoil
@01:07:13.62 delay 1 second
@01:07:14.64 AS.reconfigure ASfast
@01:07:14.70 AS.seek 0ml
@01:07:42.05 delay 3 seconds
@01:07:45.07 AFV.select :carrierSolution
@01:07:45.18 delay 2 seconds
@01:07:47.20 AS.reconfigure ASconfig
@01:07:47.26 AS.seek 1ml
@01:08:45.02 delay 1 second
@01:08:46.04 AFV.select :holdingCoil
@01:08:46.15 delay 1 second
@01:08:47.17 AS.reconfigure ASfast
@01:08:47.23 AS.seek 0ml
@01:09:14.58 delay 3 seconds
@01:09:17.60 AFV.select :carrierSolution
@01:09:17.71 delay 2 seconds
@01:09:19.73 AS.reconfigure ASconfig
@01:09:19.80 AS.seek 1ml
@01:10:17.53 delay 1 second
@01:10:18.55 AFV.select :holdingCoil
@01:10:18.66 delay 1 second
@01:10:19.68 AS.reconfigure ASfast
@01:10:19.74 AS.seek 0ml
@01:10:47.09 delay 3 seconds
@01:10:50.11 AFV.select :carrierSolution
@01:10:50.22 delay 2 seconds
@01:10:52.24 AS.reconfigure ASconfig
@01:10:52.31 AS.seek 1ml
@01:11:50.06 delay 1 second
@01:11:51.08 AFV.select :holdingCoil
@01:11:51.19 delay 1 second
@01:11:52.21 AS.reconfigure ASnorm
@01:11:52.26 AS.seek 0ml
@01:12:42.97 Setting up PCR for AIPc -- writing /var/log/esp/AIPc21apr0701h.pcr
@01:12:42.98 Building PCR Slug from ASV with MM3 mix, PP6 primer
@01:12:43.01 Priming with carrier
@01:12:43.02 ATV.dial ARV
@01:12:43.69 ARV.dial :carrier
@01:12:44.05 AS.seek 0.5ml
@01:13:09.56 AFV.select :carrierSolution
@01:13:09.69 delay 2 seconds
@01:13:11.71 AS.reconfigure ASconfig
@01:13:11.78 AS.seek 1ml
@01:13:40.78 delay 1 second
@01:13:41.80 AFV.select :holdingCoil
@01:13:41.91 delay 1 second
@01:13:42.93 ATV.dial :waste
@01:13:44.70 AS.reconfigure ASnorm
@01:13:44.76 AS.seek 0ml
@01:14:35.49 AFV.select :carrierSolution
@01:14:35.62 delay 2 seconds
@01:14:37.64 AS.reconfigure ASconfig
@01:14:37.70 AS.seek 0.5ml
@01:15:06.75 delay 1 second
@01:15:07.77 AFV.select :holdingCoil
@01:15:07.88 Building Slug
@01:15:07.89 ATV.dial :ARV
@01:15:09.63 delay 2 seconds
@01:15:11.66 ARV.dial :air1
@01:15:12.08 delay 2 seconds
@01:15:14.14 AS.reconfigure ASslow2
@01:15:14.20 AS.seek 0.506ml
@01:15:20.49 delay 2 seconds
@01:15:22.52 ARV.dial :MM3
@01:15:23.63 delay 2 seconds
@01:15:25.66 Adding MM3
@01:15:25.74 AS.seek 0.524ml
@01:15:44.36 delay 25 seconds
@01:16:09.39 ARV.dial :PP6
@01:16:09.79 Adding PP6
@01:16:09.80 delay 2 seconds
@01:16:11.87 AS.seek 0.53ml
@01:16:18.19 delay 10 seconds
@01:16:28.23 ARV.dial :air1
@01:16:29.10 adding ARV air
@01:16:29.12 delay 2 seconds
@01:16:31.19 AS.seek 0.536ml
@01:16:37.63 delay 5 seconds
@01:16:42.66 ARV.dial :carrier
@01:16:43.08 delay 7 seconds
@01:16:50.15 AS.seek 0.594ml
@01:17:48.69 delay 5 seconds
@01:17:53.72 ATV.dial :ASV
@01:17:54.14 delay 2 seconds
@01:17:56.21 AS.seek 0.6ml
@01:18:03.05 delay 4 seconds
@01:18:07.08 ATV.dial ARV
@01:18:07.64 delay 2 seconds
@01:18:09.67 ARV.dial :carrier
@01:18:09.68 delay 2 seconds
@01:18:11.75 AS.seek 0.65ml
@01:19:02.19 delay 4 seconds
@01:19:06.22 ATV.dial PCR
@01:19:07.31 ASV.dial :waste1
@01:19:07.33 ARV.dial :air1
@01:19:07.75 Positioning Slug by 0.324ml
@01:19:07.83 AS.seek 0.326ml
@01:24:34.05 delay 5 seconds
@01:24:39.08 ATV.dial ARV
@01:24:39.89 ASV.dial :PCRlock
@01:24:40.30 delay 5 seconds
@01:24:45.33 Running AIPc ProfileIPc thermocycling
@01:24:45.34 <PCR> PCR.program ProfileIPc
@01:24:45.47 ProfileIPc should complete in < 1:26:35
@01:24:45.48 PCR.run 1:53:13.75
@02:33:24.63 PCR.off
@02:33:25.20 <MFBPCR> PCR for AIPc completed -- closed /var/log/esp/AIPc21apr0701h.pcr
@02:33:25.21 Full MFB Decontamination
@02:33:25.23 Decon extraction column and decon extSrc
@02:33:25.26 SPE.hold 2:46:40,55C
@02:33:25.31 ASV.dial :SPEdelivery
@02:33:25.99 delay 20 seconds
@02:33:46.02 AFV.select :carrierSolution
@02:33:46.13 delay 2 seconds
@02:33:48.15 AS.reconfigure ASconfig
@02:33:48.21 AS.seek 0.5ml
@02:33:58.52 delay 1 second
@02:33:59.54 AFV.select :holdingCoil
@02:33:59.65 ATV.dial :air1
@02:34:01.87 AS.reconfigure ASslow1
@02:34:01.93 AS.seek 0.52ml
@02:34:06.28 ATV.dial :ASV
@02:34:08.00 ASV.dial :SPErecovery
@02:34:08.69 AS.seek 0.62ml
@02:34:29.17 ATV.dial :waste
@02:34:31.36 AS.reconfigure ASnorm
@02:34:31.42 AS.seek 0ml
@02:35:02.94 deconEluateRecoveryLine extSrc
@02:35:02.95 ATV.dial :waste
@02:35:02.98 AS.seek 0ml
@02:35:03.07 ATV.dial :air3
@02:35:03.84 AS.reconfigure ASslow1
@02:35:03.90 AS.seek 0.01ml
@02:35:06.21 delay 2 seconds
@02:35:08.24 ATV.dial :bleach
@02:35:08.73 AS.seek 0.31ml
@02:36:09.44 delay 3 seconds
@02:36:12.47 ATV.dial :air3
@02:36:13.10 delay 3 seconds
@02:36:16.17 AS.seek 0.335ml
@02:36:21.50 delay 3 seconds
@02:36:24.53 ATV.dial :bleach
@02:36:25.20 AS.seek 0.32ml
@02:36:28.53 delay 3 seconds
@02:36:31.56 ATV.dial :extSrc
@02:36:32.25 AS.seek 0ml
@02:37:36.86 AFV.select :carrierSolution
@02:37:36.98 delay 2 seconds
@02:37:39.00 AS.reconfigure ASconfig
@02:37:39.06 AS.seek 0.5ml
@02:38:08.11 delay 1 second
@02:38:09.13 AFV.select :holdingCoil
@02:38:09.24 ATV.dial :extSrc
@02:38:09.26 AS.reconfigure ASslow1
@02:38:09.33 AS.seek 0ml
@02:39:50.25 AFV.select :carrierSolution
@02:39:50.37 delay 2 seconds
@02:39:52.39 AS.reconfigure ASconfig
@02:39:52.45 AS.seek 0.5ml
@02:40:21.47 delay 1 second
@02:40:22.49 AFV.select :holdingCoil
@02:40:22.60 ATV.dial :extSrc
@02:40:22.62 AS.reconfigure ASslow1
@02:40:22.69 AS.seek 0ml
@02:42:03.52 AFV.select :carrierSolution
@02:42:03.64 delay 2 seconds
@02:42:05.66 AS.reconfigure ASconfig
@02:42:05.72 AS.seek 0.5ml
@02:42:34.72 delay 1 second
@02:42:35.74 AFV.select :holdingCoil
@02:42:35.85 ATV.dial :extSrc
@02:42:35.87 AS.reconfigure ASslow1
@02:42:35.94 AS.seek 0ml
@02:44:16.77 AFV.select :carrierSolution
@02:44:16.89 delay 2 seconds
@02:44:18.91 AS.reconfigure ASconfig
@02:44:18.97 AS.seek 0.5ml
@02:44:47.99 delay 1 second
@02:44:49.01 AFV.select :holdingCoil
@02:44:49.12 ATV.dial :extSrc
@02:44:49.14 AS.reconfigure ASslow1
@02:44:49.21 AS.seek 0ml
@02:46:30.05 ATV.dial :air2
@02:46:31.45 AS.reconfigure ASslow0
@02:46:31.51 AS.seek 0.5ml
@02:47:22.06 ATV.dial :extSrc
@02:47:23.63 AS.seek 0.02ml
@02:48:12.25 delay 1 second
@02:48:13.28 ATV.dial :waste
@02:48:14.64 AS.reconfigure ASconfig
@02:48:14.70 AS.seek 0ml
@02:48:16.16 AFV.select :holdingCoil
@02:48:16.18 ATV.dial :waste
@02:48:16.21 AS.seek 0ml
@02:48:16.29 AFV.select :carrierSolution
@02:48:16.41 delay 2 seconds
@02:48:18.43 AS.seek 0.5ml
@02:48:47.44 delay 1 second
@02:48:48.46 AFV.select :holdingCoil
@02:48:48.57 ATV.dial :air3
@02:48:49.42 AS.reconfigure ASslow1
@02:48:49.48 AS.seek 0.52ml
@02:48:53.77 ATV.dial :air3
@02:48:53.86 AS.seek 0.53ml
@02:48:56.14 delay 2 seconds
@02:48:58.17 ATV.dial :bleach
@02:48:58.64 AS.seek 0.71ml
@02:49:35.19 delay 3 seconds
@02:49:38.22 ATV.dial :air3
@02:49:39.24 delay 3 seconds
@02:49:42.32 AS.seek 0.735ml
@02:49:47.65 delay 3 seconds
@02:49:50.68 ATV.dial :bleach
@02:49:51.33 AS.seek 0.72ml
@02:49:54.60 delay 3 seconds
@02:49:57.63 ATV.dial ASV
@02:49:58.72 ASV.dial :SPErecovery
@02:49:58.81 AS.seek 0.365ml
@02:51:10.46 ATV.dial :waste
@02:51:12.48 AS.reconfigure ASnorm
@02:51:12.54 AS.seek 0ml
@02:51:31.28 ATV.dial :air3
@02:51:32.16 AS.reconfigure ASslow1
@02:51:32.22 AS.seek 0.01ml
@02:51:34.63 delay 2 seconds
@02:51:36.66 ATV.dial :bleach
@02:51:37.16 AS.seek 0.58ml
@02:53:32.07 delay 3 seconds
@02:53:35.10 ATV.dial :air3
@02:53:35.67 delay 3 seconds
@02:53:38.74 AS.seek 0.605ml
@02:53:44.21 delay 3 seconds
@02:53:47.24 ATV.dial :bleach
@02:53:47.88 AS.seek 0.59ml
@02:53:51.17 delay 3 seconds
@02:53:54.20 ATV.dial :ASV
@02:53:55.30 ASV.dial :wasteR
@02:53:55.72 AS.seek 0ml
@02:55:54.77 delay 1:00
@02:56:54.81 ATV.dial ASV
@02:56:54.82 AFV.select :carrierSolution
@02:56:54.94 delay 2 seconds
@02:56:56.96 AS.reconfigure ASconfig
@02:56:57.02 AS.seek 1ml
@02:57:54.77 delay 1 second
@02:57:55.79 AFV.select :holdingCoil
@02:57:55.90 delay 1 second
@02:57:56.92 ASV.dial :wasteR
@02:57:56.93 AS.reconfigure ASslow1
@02:57:57.01 AS.seek 0ml
@03:01:18.41 delay 10 seconds
@03:01:28.45 ATV.dial :air3
@03:01:30.05 AS.seek 0.01ml
@03:01:32.38 delay 2 seconds
@03:01:34.41 ATV.dial :bleach
@03:01:35.06 AS.seek 0.58ml
@03:03:29.94 delay 3 seconds
@03:03:32.97 ATV.dial :air3
@03:03:33.55 delay 3 seconds
@03:03:36.62 AS.seek 0.605ml
@03:03:41.93 delay 3 seconds
@03:03:44.96 ATV.dial :bleach
@03:03:45.59 AS.seek 0.59ml
@03:03:48.85 delay 3 seconds
@03:03:51.88 ATV.dial ASV
@03:03:52.99 ASV.dial :SPEdelivery
@03:03:53.39 AS.seek 0ml
@03:05:52.27 delay 1:00
@03:06:52.30 AFV.select :carrierSolution
@03:06:52.41 delay 2 seconds
@03:06:54.43 AS.reconfigure ASconfig
@03:06:54.49 AS.seek 1ml
@03:07:52.25 delay 1 second
@03:07:53.27 AFV.select :holdingCoil
@03:07:53.38 delay 1 second
@03:07:54.40 ATV.dial ASV
@03:07:54.41 ASV.dial :SPEdelivery
@03:07:54.44 AS.reconfigure ASslow1
@03:07:54.50 AS.seek 0ml
@03:11:15.92 delay 10 seconds
@03:11:25.96 ATV.dial :air2
@03:11:26.84 AS.reconfigure ASslow0
@03:11:26.90 AS.seek 0.8ml
@03:12:47.71 delay 3 seconds
@03:12:50.74 ATV.dial :ASV
@03:12:51.78 ASV.dial :SPEdelivery
@03:12:51.80 AS.reconfigure ASslow1
@03:12:51.87 AS.seek 0.03ml
@03:15:27.02 delay 20 seconds
@03:15:47.06 ATV.dial :waste,via: :air3
@03:15:48.90 AS.reconfigure ASconfig
@03:15:48.95 AS.seek 0ml
@03:15:50.97 SPE.coast
@03:15:51.06 Removing Bleach from Mixing Coil
@03:15:51.07 AFV.select :carrierSolution
@03:15:51.19 delay 2 seconds
@03:15:53.21 AS.seek 0.5ml
@03:16:22.24 delay 1 second
@03:16:23.26 AFV.select :holdingCoil
@03:16:23.37 ATV.dial :ASV
@03:16:25.32 ASV.dial :SPErecovery
@03:16:25.98 AS.reconfigure ASslow1
@03:16:26.04 AS.seek 0.73ml
@03:17:12.57 ASV.dial :wasteD
@03:17:13.18 AS.reconfigure ASnorm
@03:17:13.23 AS.seek 0ml
@03:17:50.35 AFV.select :carrierSolution
@03:17:50.47 delay 2 seconds
@03:17:52.49 AS.reconfigure ASconfig
@03:17:52.55 AS.seek 0.5ml
@03:18:21.61 delay 1 second
@03:18:22.63 AFV.select :holdingCoil
@03:18:22.74 ATV.dial ASV
@03:18:22.75 ASV.dial :SPErecovery
@03:18:23.42 AS.reconfigure ASslow0
@03:18:23.48 AS.seek 0.3ml
@03:18:43.91 ATV.dial :air2
@03:18:45.29 AS.reconfigure ASslow1
@03:18:45.35 AS.seek 0.32ml
@03:18:49.77 ATV.dial ASV
@03:18:50.88 AS.reconfigure ASslow0
@03:18:50.94 AS.seek 0.57ml
@03:19:16.44 ASV.dial :wasteD
@03:19:17.06 AS.reconfigure ASnorm
@03:19:17.12 AS.seek 0ml
@03:19:46.19 AFV.select :carrierSolution
@03:19:46.31 delay 2 seconds
@03:19:48.33 AS.reconfigure ASconfig
@03:19:48.39 AS.seek 0.5ml
@03:20:17.46 delay 1 second
@03:20:18.48 AFV.select :holdingCoil
@03:20:18.59 ATV.dial ASV
@03:20:18.60 ASV.dial :SPErecovery
@03:20:19.27 AS.reconfigure ASslow0
@03:20:19.33 AS.seek 0.3ml
@03:20:39.88 ATV.dial :air2
@03:20:40.99 AS.reconfigure ASslow1
@03:20:41.05 AS.seek 0.32ml
@03:20:45.52 ATV.dial ASV
@03:20:46.63 AS.reconfigure ASslow0
@03:20:46.69 AS.seek 0.57ml
@03:21:12.19 ASV.dial :wasteD
@03:21:12.81 AS.reconfigure ASnorm
@03:21:12.87 AS.seek 0ml
@03:21:41.94 ATV.dial :air2
@03:21:43.00 AS.reconfigure ASslow0
@03:21:43.06 AS.seek 0.3ml
@03:22:13.58 ATV.dial :ASV
@03:22:14.61 ASV.dial :wasteD
@03:22:14.63 AS.reconfigure ASconfig
@03:22:14.70 AS.seek 0.05ml
@03:22:29.39 ATV.dial :waste
@03:22:31.39 AS.seek 0ml
@03:22:34.59 ASV.dial :SPEdelivery
@03:22:35.68 Cleaning PCR
@03:22:35.69 AFV.select :carrierSolution
@03:22:35.81 delay 2 seconds
@03:22:37.83 AS.seek 0.5ml
@03:23:06.86 delay 1 second
@03:23:07.88 AFV.select :holdingCoil
@03:23:07.99 ASV.dial :waste1
@03:23:08.36 ATV.dial :air3
@03:23:09.08 AS.reconfigure ASslow1
@03:23:09.14 AS.seek 0.51ml
@03:23:11.41 delay 2 seconds
@03:23:13.44 ATV.dial :bleach
@03:23:13.91 AS.seek 0.81ml
@03:24:14.50 delay 3 seconds
@03:24:17.53 ATV.dial :air3
@03:24:18.11 delay 3 seconds
@03:24:21.18 AS.seek 0.835ml
@03:24:26.59 delay 3 seconds
@03:24:29.62 ATV.dial :bleach
@03:24:30.25 AS.seek 0.82ml
@03:24:33.53 delay 3 seconds
@03:24:36.56 ARV.dial :waste
@03:24:37.19 delay 3 seconds
@03:24:40.22 ATV.dial :ARV
@03:24:41.15 AS.reconfigure ASconfig
@03:24:41.21 AS.seek 0.47ml
@03:25:01.63 AFV.select :carrierSolution
@03:25:01.75 delay 2 seconds
@03:25:03.77 AS.seek 1ml
@03:25:34.57 delay 1 second
@03:25:35.59 AFV.select :holdingCoil
@03:25:35.70 delay 1 second
@03:25:36.72 AS.reconfigure ASfast
@03:25:36.78 AS.seek 0ml
@03:26:04.11 AFV.select :carrierSolution
@03:26:04.23 delay 2 seconds
@03:26:06.25 AS.reconfigure ASconfig
@03:26:06.31 AS.seek 1ml
@03:27:04.07 delay 1 second
@03:27:05.09 AFV.select :holdingCoil
@03:27:05.20 delay 1 second
@03:27:06.22 AS.reconfigure ASfast
@03:27:06.28 AS.seek 0ml
@03:27:33.63 AFV.select :carrierSolution
@03:27:33.75 delay 2 seconds
@03:27:35.77 AS.reconfigure ASconfig
@03:27:35.83 AS.seek 1ml
@03:28:33.57 delay 1 second
@03:28:34.59 AFV.select :holdingCoil
@03:28:34.70 delay 1 second
@03:28:35.72 AS.reconfigure ASfast
@03:28:35.78 AS.seek 0ml
@03:29:03.13 ARV.dial between carrier and air1
@03:29:03.64 ATV.dial :air3
@03:29:04.99 AS.reconfigure ASslow1
@03:29:05.05 AS.seek 0.01ml
@03:29:07.46 delay 2 seconds
@03:29:09.49 ATV.dial :bleach
@03:29:10.14 AS.seek 0.81ml
@03:31:51.28 delay 3 seconds
@03:31:54.31 ATV.dial :air3
@03:31:54.89 delay 3 seconds
@03:31:57.96 AS.seek 0.835ml
@03:32:03.25 delay 3 seconds
@03:32:06.28 ATV.dial :bleach
@03:32:06.92 AS.seek 0.82ml
@03:32:10.21 delay 3 seconds
@03:32:13.24 ATV.dial PCR
@03:32:13.65 AS.reconfigure ASconfig
@03:32:13.70 AS.seek 0ml
@03:33:01.55 PCR.program :bleachProfile
@03:33:01.63 PCR program should complete in < 2:45
@03:33:01.64 PCR.run 10:00
@03:36:03.48 PCR.off
@03:36:03.51 AFV.select :carrierSolution
@03:36:03.63 delay 2 seconds
@03:36:05.65 AS.seek 1ml
@03:37:03.42 delay 1 second
@03:37:04.44 AFV.select :holdingCoil
@03:37:04.55 delay 1 second
@03:37:05.57 AS.reconfigure ASfast
@03:37:05.63 AS.seek 0ml
@03:37:32.97 delay 3 seconds
@03:37:35.99 AFV.select :carrierSolution
@03:37:36.10 delay 2 seconds
@03:37:38.12 AS.reconfigure ASconfig
@03:37:38.18 AS.seek 1ml
@03:38:35.94 delay 1 second
@03:38:36.96 AFV.select :holdingCoil
@03:38:37.07 delay 1 second
@03:38:38.09 AS.reconfigure ASfast
@03:38:38.15 AS.seek 0ml
@03:39:05.49 delay 3 seconds
@03:39:08.51 AFV.select :carrierSolution
@03:39:08.62 delay 2 seconds
@03:39:10.64 AS.reconfigure ASconfig
@03:39:10.70 AS.seek 1ml
@03:40:08.49 delay 1 second
@03:40:09.51 AFV.select :holdingCoil
@03:40:09.62 delay 1 second
@03:40:10.64 AS.reconfigure ASfast
@03:40:10.70 AS.seek 0ml
@03:40:38.03 delay 3 seconds
@03:40:41.05 AFV.select :carrierSolution
@03:40:41.16 delay 2 seconds
@03:40:43.18 AS.reconfigure ASconfig
@03:40:43.24 AS.seek 1ml
@03:41:41.00 delay 1 second
@03:41:42.02 AFV.select :holdingCoil
@03:41:42.13 delay 1 second
@03:41:43.15 AS.reconfigure ASfast
@03:41:43.21 AS.seek 0ml
@03:42:10.55 delay 3 seconds
@03:42:13.57 AFV.select :carrierSolution
@03:42:13.68 delay 2 seconds
@03:42:15.70 AS.reconfigure ASconfig
@03:42:15.76 AS.seek 1ml
@03:43:13.54 delay 1 second
@03:43:14.55 AFV.select :holdingCoil
@03:43:14.69 delay 1 second
@03:43:15.72 AS.reconfigure ASnorm
@03:43:15.78 AS.seek 0ml
@03:44:06.49 AFV.select :carrierSolution
@03:44:06.61 delay 2 seconds
@03:44:08.63 AS.reconfigure ASconfig
@03:44:08.68 AS.seek 1ml
@03:45:06.47 delay 1 second
@03:45:07.50 AFV.select :holdingCoil
@03:45:07.67 delay 1 second
@03:45:08.70 ATV.dial :waste
@03:45:10.03 AS.reconfigure ASnorm
@03:45:10.08 AS.seek 0.5ml
@03:45:35.63 ATV.dial :waste2
@03:45:37.00 AS.seek 0ml
@03:46:02.58 <bspe> Session Ended
@06:19:30.56CEST07-Apr-21 <MAIN> ESP2 v3.30 I2C Gateway v3.06 for core bus
@06:19:32.93 
Configuration for mer 7/20/20 brent@mbari.org
/home/esp/esp2/type/shallow/mer/configure.rb
@06:19:33.02 
Configuration for MFB3 by preston@mbari.org
/home/esp/esp2/type/mfb/MFB3/configure.rb
@06:19:37.85 Polling Can every 10:00
@06:19:40.02 CS.configure :default
@06:19:40.06 CS.hold
@06:19:40.12 CS.configure :default
@06:19:40.20 PS.configure :default
@06:19:40.28 SS.configure :default
@06:19:40.32 SS.hold
@06:19:40.36 SS.configure :default
@06:19:40.44 CC.configure :default
@06:19:40.48 CC.hold
@06:19:40.55 CC.configure :default
@06:19:40.63 PC.configure :default
@06:19:40.67 PC.hold
@06:19:40.71 PC.configure :default
@06:19:40.81 Forearm.configure :loose
@06:19:40.85 Forearm.hold
@06:19:40.89 Forearm.configure :loose
@06:19:40.97 Elbow.configure :loose
@06:19:41.01 Elbow.hold
@06:19:41.07 Elbow.configure :loose
@06:19:41.17 SC.configure :loose
@06:19:41.21 SC.hold
@06:19:41.26 SC.configure :loose
@06:19:41.33 SE.configure :default
@06:19:41.37 SE.hold
@06:19:41.41 SE.configure :default
@06:19:41.45 CH.coast
@06:19:41.49 CH.configure :default
@06:19:41.52 PH.coast
@06:19:41.58 PH.configure :default
@06:19:41.68 SPE.coast
@06:19:41.72 SPE.configure SPEconfig
@06:19:41.79 AS.configure ASconfig
@06:19:41.83 AS.hold
@06:19:41.88 AS.configure ASconfig
@06:19:41.92 PCR.off
@06:19:41.96 PCR.pidtemp= 50,0.1,0,300,25,25
@06:19:42.00 PCR.caltemp= 2.5051,0.0372,0
@06:19:42.04 All dwarf controllers running firmware version 3.75
@06:19:42.24 Cleanly exited at 03:46:02CEST07-Apr-21
@06:19:42.27 Loading /home/esp/esp2/mission/GENOMAP_v2.rb mission
@06:19:42.28 SyntaxError in MAIN -- /home/esp/esp2/mission/GENOMAP_v2.rb:1: syntax error, unexpected '\n', expecting tCOLON2 or '[' or '.'
/home/esp/esp2/mission/GENOMAP_v2.rb:4: syntax error, unexpected tINTEGER, expecting kDO or '{' or '('
3.times{pcrDecon, delay 5}; 
                         ^
@06:19:42.30 Waiting to Resume from Checkpoint "Loading /home/esp/esp2/mission/GENOMAP_v2.rb mission"
@06:19:42.31 <Qmail> BadNews.email "/home/esp/esp2/mission/GENOMAP_v2.rb:1: syntax error, unexpected '\\n', expecting tCOLON2 or '[' or '.'\n/home/esp/esp2/mission/GENOMAP_v2.rb:4: syntax error, unexpected tINTEGER, expecting kDO or '{' or '('\n3.times{pcrDecon, delay 5}; \n                         ^",Subject: "SyntaxError in MAIN"
@06:19:43.45 <mwj> Session Begun
@06:20:47.55 -> showlog
@06:21:26.10 -> abort! "syntax error"
@06:21:26.11 CmdServer::Client::Error in mwj -- Aborting Mission: syntax error
@06:21:26.16 <Qmail> BadNews.email "syntax error",Subject: "Mission ABORTED!"
@06:21:32.46 <mwj> Session Ended
@06:29:09.04CEST07-Apr-21 <MAIN> ESP2 v3.30 I2C Gateway v3.06 for core bus
@06:29:11.99 
Configuration for mer 7/20/20 brent@mbari.org
/home/esp/esp2/type/shallow/mer/configure.rb
@06:29:12.16 
Configuration for MFB3 by preston@mbari.org
/home/esp/esp2/type/mfb/MFB3/configure.rb
Log::Locked in trapHandler -- Another process is already writing to /var/log/esp/real.log
@06:29:20.17 Polling Can every 10:00
@06:29:22.32 CS.configure :default
@06:29:22.36 CS.hold
@06:29:22.43 CS.configure :default
@06:29:22.51 PS.configure :default
@06:29:22.59 SS.configure :default
@06:29:22.63 SS.hold
@06:29:22.67 SS.configure :default
@06:29:22.76 CC.configure :default
@06:29:22.79 CC.hold
@06:29:22.84 CC.configure :default
@06:29:22.92 PC.configure :default
@06:29:22.95 PC.hold
@06:29:23.00 PC.configure :default
@06:29:23.09 Forearm.configure :loose
@06:29:23.13 Forearm.hold
@06:29:23.19 Forearm.configure :loose
@06:29:23.27 Elbow.configure :loose
@06:29:23.31 Elbow.hold
@06:29:23.36 Elbow.configure :loose
@06:29:23.46 SC.configure :loose
@06:29:23.50 SC.hold
@06:29:23.54 SC.configure :loose
@06:29:23.62 SE.configure :default
@06:29:23.66 SE.hold
@06:29:23.72 SE.configure :default
@06:29:23.76 CH.coast
@06:29:23.80 CH.configure :default
@06:29:23.83 PH.coast
@06:29:23.88 PH.configure :default
@06:29:23.98 SPE.coast
@06:29:24.03 SPE.configure SPEconfig
@06:29:24.11 AS.configure ASconfig
@06:29:24.14 AS.hold
@06:29:24.19 AS.configure ASconfig
@06:29:24.24 PCR.off
@06:29:24.27 PCR.pidtemp= 50,0.1,0,300,25,25
@06:29:24.30 PCR.caltemp= 2.5051,0.0372,0
@06:29:24.33 All dwarf controllers running firmware version 3.75
@06:29:24.53 Cleanly exited at 06:21:32CEST07-Apr-21
@06:29:24.56 Loading /home/esp/esp2/mission/GENOMAP_v2.rb mission
@06:29:24.57 SyntaxError in MAIN -- /home/esp/esp2/mission/GENOMAP_v2.rb:4: syntax error, unexpected tINTEGER, expecting kDO or '{' or '('
1.times{pcrDecon, delay 5}; 
                         ^
@06:29:24.60 <Qmail> BadNews.email "/home/esp/esp2/mission/GENOMAP_v2.rb:4: syntax error, unexpected tINTEGER, expecting kDO or '{' or '('\n1.times{pcrDecon, delay 5}; \n                         ^",Subject: "SyntaxError in MAIN"
@06:29:24.61 <MAIN> Waiting to Resume from Checkpoint "Loading /home/esp/esp2/mission/GENOMAP_v2.rb mission"
@06:29:25.63 <mwj> Session Begun
@06:30:03.89 -> abort! "syntax error again"
@06:30:03.90 CmdServer::Client::Error in mwj -- Aborting Mission: syntax error again
@06:30:03.95 <Qmail> BadNews.email "syntax error again",Subject: "Mission ABORTED!"
@06:30:09.92 <mwj> Session Ended
@06:39:42.64CEST07-Apr-21 <MAIN> ESP2 v3.30 I2C Gateway v3.06 for core bus
@06:39:45.16 
Configuration for mer 7/20/20 brent@mbari.org
/home/esp/esp2/type/shallow/mer/configure.rb
@06:39:45.25 
Configuration for MFB3 by preston@mbari.org
/home/esp/esp2/type/mfb/MFB3/configure.rb
@06:39:50.03 Polling Can every 10:00
@06:39:51.74 CS.configure :default
@06:39:51.78 CS.hold
@06:39:51.85 CS.configure :default
@06:39:51.93 PS.configure :default
@06:39:52.02 SS.configure :default
@06:39:52.06 SS.hold
@06:39:52.11 SS.configure :default
@06:39:52.19 CC.configure :default
@06:39:52.23 CC.hold
@06:39:52.28 CC.configure :default
@06:39:52.36 PC.configure :default
@06:39:52.40 PC.hold
@06:39:52.47 PC.configure :default
@06:39:52.57 Forearm.configure :loose
@06:39:52.61 Forearm.hold
@06:39:52.65 Forearm.configure :loose
@06:39:52.73 Elbow.configure :loose
@06:39:52.77 Elbow.hold
@06:39:52.81 Elbow.configure :loose
@06:39:52.91 SC.configure :loose
@06:39:52.95 SC.hold
@06:39:53.00 SC.configure :loose
@06:39:53.08 SE.configure :default
@06:39:53.12 SE.hold
@06:39:53.18 SE.configure :default
@06:39:53.25 CH.coast
@06:39:53.34 CH.configure :default
@06:39:53.39 PH.coast
@06:39:53.49 PH.configure :default
@06:39:53.69 SPE.coast
@06:39:53.78 SPE.configure SPEconfig
@06:39:53.93 AS.configure ASconfig
@06:39:54.01 AS.hold
@06:39:54.08 AS.configure ASconfig
@06:39:54.13 PCR.off
@06:39:54.16 PCR.pidtemp= 50,0.1,0,300,25,25
@06:39:54.19 PCR.caltemp= 2.5051,0.0372,0
@06:39:54.22 All dwarf controllers running firmware version 3.75
@06:39:54.41 Cleanly exited at 06:39:13CEST07-Apr-21
@06:39:54.44 Loading /home/esp/esp2/mission/GENOMAP_v2.rb mission
@06:39:54.49 Cleaning PCR
@06:39:54.50 AFV.select :carrierSolution
@06:39:54.60 delay 2 seconds
@06:39:56.62 AS.seek 0.5ml
@06:39:58.01 <mwj> Session Begun
@06:40:25.62 <MAIN> delay 1 second
@06:40:26.64 AFV.select :holdingCoil
@06:40:26.75 ASV.dial :waste1
@06:40:26.82 ATV.dial :air3
@06:40:28.88 AS.reconfigure ASslow1
@06:40:28.95 AS.seek 0.51ml
@06:40:31.19 delay 2 seconds
@06:40:32.40 <mwj> -> exit
@06:40:32.41 Session Ended
@06:40:33.22 <MAIN> ATV.dial :bleach
@06:40:33.68 AS.seek 0.81ml
@06:41:34.33 delay 3 seconds
@06:41:37.36 ATV.dial :air3
@06:41:37.93 delay 3 seconds
@06:41:41.00 AS.seek 0.835ml
@06:41:46.29 delay 3 seconds
@06:41:49.32 ATV.dial :bleach
@06:41:49.94 AS.seek 0.82ml
@06:41:53.22 delay 3 seconds
@06:41:56.25 ARV.dial :waste
@06:41:56.76 delay 3 seconds
@06:41:59.79 ATV.dial :ARV
@06:42:00.69 AS.reconfigure ASconfig
@06:42:00.73 AS.seek 0.47ml
@06:42:21.13 AFV.select :carrierSolution
@06:42:21.24 delay 2 seconds
@06:42:23.26 AS.seek 1ml
@06:42:54.04 delay 1 second
@06:42:55.06 AFV.select :holdingCoil
@06:42:55.17 delay 1 second
@06:42:56.19 AS.reconfigure ASfast
@06:42:56.23 AS.seek 0ml
@06:43:23.55 AFV.select :carrierSolution
@06:43:23.66 delay 2 seconds
@06:43:25.68 AS.reconfigure ASconfig
@06:43:25.73 AS.seek 1ml
@06:44:23.46 delay 1 second
@06:44:24.47 AFV.select :holdingCoil
@06:44:24.58 delay 1 second
@06:44:25.60 AS.reconfigure ASfast
@06:44:25.65 AS.seek 0ml
@06:44:52.99 AFV.select :carrierSolution
@06:44:53.10 delay 2 seconds
@06:44:55.12 AS.reconfigure ASconfig
@06:44:55.17 AS.seek 1ml
@06:45:53.26 delay 1 second
@06:45:54.32 AFV.select :holdingCoil
@06:45:54.43 delay 1 second
@06:45:55.45 AS.reconfigure ASfast
@06:45:55.50 AS.seek 0ml
@06:46:22.83 ARV.dial between carrier and air1
@06:46:23.36 ATV.dial :air3
@06:46:24.69 AS.reconfigure ASslow1
@06:46:24.73 AS.seek 0.01ml
@06:46:27.02 delay 2 seconds
@06:46:29.05 ATV.dial :bleach
@06:46:29.68 AS.seek 0.81ml
@06:49:10.80 delay 3 seconds
@06:49:13.83 ATV.dial :air3
@06:49:14.40 delay 3 seconds
@06:49:17.47 AS.seek 0.835ml
@06:49:22.77 delay 3 seconds
@06:49:25.80 ATV.dial :bleach
@06:49:26.43 AS.seek 0.82ml
@06:49:29.74 delay 3 seconds
@06:49:32.77 ATV.dial PCR
@06:49:33.19 AS.reconfigure ASconfig
@06:49:33.23 AS.seek 0ml
@06:50:20.63 PCR.program :bleachProfile
@06:50:20.69 PCR program should complete in < 2:45
@06:50:20.70 PCR.run 10:00
@06:53:22.54 PCR.off
@06:53:22.56 AFV.select :carrierSolution
@06:53:22.67 delay 2 seconds
@06:53:24.69 AS.seek 1ml
@06:54:22.46 delay 1 second
@06:54:23.47 AFV.select :holdingCoil
@06:54:23.58 delay 1 second
@06:54:24.60 AS.reconfigure ASfast
@06:54:24.65 AS.seek 0ml
@06:54:51.98 delay 3 seconds
@06:54:55.00 AFV.select :carrierSolution
@06:54:55.11 delay 2 seconds
@06:54:57.13 AS.reconfigure ASconfig
@06:54:57.18 AS.seek 1ml
@06:55:54.96 delay 1 second
@06:55:55.98 AFV.select :holdingCoil
@06:55:56.09 delay 1 second
@06:55:57.11 AS.reconfigure ASfast
@06:55:57.16 AS.seek 0ml
@06:56:24.48 delay 3 seconds
@06:56:27.50 AFV.select :carrierSolution
@06:56:27.61 delay 2 seconds
@06:56:29.63 AS.reconfigure ASconfig
@06:56:29.69 AS.seek 1ml
@06:57:27.46 delay 1 second
@06:57:28.47 AFV.select :holdingCoil
@06:57:28.58 delay 1 second
@06:57:29.60 AS.reconfigure ASfast
@06:57:29.65 AS.seek 0ml
@06:57:56.99 delay 3 seconds
@06:58:00.01 AFV.select :carrierSolution
@06:58:00.12 delay 2 seconds
@06:58:02.14 AS.reconfigure ASconfig
@06:58:02.19 AS.seek 1ml
@06:58:59.94 delay 1 second
@06:59:00.95 AFV.select :holdingCoil
@06:59:01.06 delay 1 second
@06:59:02.08 AS.reconfigure ASfast
@06:59:02.13 AS.seek 0ml
@06:59:29.44 delay 3 seconds
@06:59:32.46 AFV.select :carrierSolution
@06:59:32.57 delay 2 seconds
@06:59:34.59 AS.reconfigure ASconfig
@06:59:34.65 AS.seek 1ml
@07:00:32.43 delay 1 second
@07:00:33.44 AFV.select :holdingCoil
@07:00:33.54 delay 1 second
@07:00:34.56 AS.reconfigure ASnorm
@07:00:34.61 AS.seek 0ml
@07:01:25.31 delay 5 seconds
@07:01:30.48 <MFBPCR> Setting up PCR for AIPc -- writing /var/log/esp/AIPc21apr0707h.pcr
@07:01:30.50 Building PCR Slug from elutionWater with MM3 mix, PP6 primer
@07:01:30.51 Priming with carrier
@07:01:30.52 ATV.dial ARV
@07:01:31.15 ARV.dial :carrier
@07:01:31.49 AS.seek 0.5ml
@07:01:57.05 AFV.select :carrierSolution
@07:01:57.16 delay 2 seconds
@07:01:59.18 AS.reconfigure ASconfig
@07:01:59.23 AS.seek 1ml
@07:02:28.24 delay 1 second
@07:02:29.26 AFV.select :holdingCoil
@07:02:29.37 delay 1 second
@07:02:30.39 ATV.dial :waste
@07:02:32.13 AS.reconfigure ASnorm
@07:02:32.17 AS.seek 0ml
@07:03:22.83 AFV.select :carrierSolution
@07:03:22.94 delay 2 seconds
@07:03:24.96 AS.reconfigure ASconfig
@07:03:25.01 AS.seek 0.5ml
@07:03:54.04 delay 1 second
@07:03:55.06 AFV.select :holdingCoil
@07:03:55.17 Building Slug
@07:03:55.18 ATV.dial :ARV
@07:03:57.02 delay 2 seconds
@07:03:59.37 ARV.dial :air1
@07:03:59.80 delay 2 seconds
@07:04:01.87 AS.reconfigure ASslow2
@07:04:01.92 AS.seek 0.506ml
@07:04:08.24 delay 2 seconds
@07:04:10.27 ARV.dial :MM3
@07:04:11.35 delay 2 seconds
@07:04:13.38 Adding MM3
@07:04:13.44 AS.seek 0.524ml
@07:04:31.77 delay 25 seconds
@07:04:56.80 ARV.dial :PP6
@07:04:57.19 Adding PP6
@07:04:57.20 delay 2 seconds
@07:04:59.27 AS.seek 0.53ml
@07:05:05.96 delay 10 seconds
@07:05:16.00 ARV.dial :air1
@07:05:16.86 adding ARV air
@07:05:16.87 delay 2 seconds
@07:05:18.93 AS.seek 0.536ml
@07:05:25.35 delay 5 seconds
@07:05:30.38 ARV.dial :carrier
@07:05:30.77 delay 7 seconds
@07:05:37.84 AS.seek 0.594ml
@07:06:36.58 delay 5 seconds
@07:06:41.61 ATV.dial :elutionWater
@07:06:43.10 delay 2 seconds
@07:06:45.17 AS.seek 0.6ml
@07:06:51.65 delay 4 seconds
@07:06:55.68 ATV.dial ARV
@07:06:57.15 delay 2 seconds
@07:06:59.18 ARV.dial :carrier
@07:06:59.19 delay 2 seconds
@07:07:01.25 AS.seek 0.65ml
@07:07:51.96 delay 4 seconds
@07:07:55.99 ATV.dial PCR
@07:07:56.77 ASV.dial :waste1
@07:07:56.79 ARV.dial :air1
@07:07:57.17 Positioning Slug by 0.324ml
@07:07:57.23 AS.seek 0.326ml
@07:13:23.48 delay 5 seconds
@07:13:28.51 ATV.dial ARV
@07:13:29.40 ASV.dial :PCRlock
@07:13:29.79 delay 5 seconds
@07:13:34.82 Running AIPc ProfileIPc thermocycling
@07:13:34.83 <PCR> PCR.program ProfileIPc
@07:13:34.94 ProfileIPc should complete in < 1:26:35
@07:13:34.95 PCR.run 1:53:13.75
@08:22:52.47 PCR.off
@08:22:53.02 <MFBPCR> PCR for AIPc completed -- closed /var/log/esp/AIPc21apr0707h.pcr
@08:22:53.08 Cleaning PCR
@08:22:53.09 AFV.select :carrierSolution
@08:22:53.20 delay 2 seconds
@08:22:55.22 AS.reconfigure ASconfig
@08:22:55.27 AS.seek 0.5ml
@08:23:05.54 delay 1 second
@08:23:06.56 AFV.select :holdingCoil
@08:23:06.67 ASV.dial :waste1
@08:23:07.05 ATV.dial :air3
@08:23:08.38 AS.reconfigure ASslow1
@08:23:08.42 AS.seek 0.51ml
@08:23:10.70 delay 2 seconds
@08:23:12.72 ATV.dial :bleach
@08:23:13.35 AS.seek 0.81ml
@08:24:13.91 delay 3 seconds
@08:24:16.94 ATV.dial :air3
@08:24:17.51 delay 3 seconds
@08:24:20.58 AS.seek 0.835ml
@08:24:25.85 delay 3 seconds
@08:24:28.88 ATV.dial :bleach
@08:24:29.51 AS.seek 0.82ml
@08:24:32.80 delay 3 seconds
@08:24:35.83 ARV.dial :waste
@08:24:36.45 delay 3 seconds
@08:24:39.48 ATV.dial :ARV
@08:24:40.38 AS.reconfigure ASconfig
@08:24:40.42 AS.seek 0.47ml
@08:25:00.80 AFV.select :carrierSolution
@08:25:00.91 delay 2 seconds
@08:25:02.93 AS.seek 1ml
@08:25:33.66 delay 1 second
@08:25:34.69 AFV.select :holdingCoil
@08:25:34.79 delay 1 second
@08:25:35.81 AS.reconfigure ASfast
@08:25:35.86 AS.seek 0ml
@08:26:03.18 AFV.select :carrierSolution
@08:26:03.29 delay 2 seconds
@08:26:05.31 AS.reconfigure ASconfig
@08:26:05.36 AS.seek 1ml
@08:27:03.13 delay 1 second
@08:27:04.15 AFV.select :holdingCoil
@08:27:04.26 delay 1 second
@08:27:05.28 AS.reconfigure ASfast
@08:27:05.33 AS.seek 0ml
@08:27:32.66 AFV.select :carrierSolution
@08:27:32.77 delay 2 seconds
@08:27:34.79 AS.reconfigure ASconfig
@08:27:34.84 AS.seek 1ml
@08:28:32.59 delay 1 second
@08:28:33.61 AFV.select :holdingCoil
@08:28:33.72 delay 1 second
@08:28:34.74 AS.reconfigure ASfast
@08:28:34.79 AS.seek 0ml
@08:29:02.13 ARV.dial between carrier and air1
@08:29:02.65 ATV.dial :air3
@08:29:03.97 AS.reconfigure ASslow1
@08:29:04.01 AS.seek 0.01ml
@08:29:06.48 delay 2 seconds
@08:29:08.51 ATV.dial :bleach
@08:29:09.13 AS.seek 0.81ml
@08:31:50.26 delay 3 seconds
@08:31:53.29 ATV.dial :air3
@08:31:53.85 delay 3 seconds
@08:31:56.92 AS.seek 0.835ml
@08:32:02.21 delay 3 seconds
@08:32:05.24 ATV.dial :bleach
@08:32:05.85 AS.seek 0.82ml
@08:32:09.10 delay 3 seconds
@08:32:12.13 ATV.dial PCR
@08:32:12.53 AS.reconfigure ASconfig
@08:32:12.57 AS.seek 0ml
@08:32:59.98 PCR.program :bleachProfile
@08:33:00.05 PCR program should complete in < 2:45
@08:33:00.06 PCR.run 10:00
@08:35:56.21 PCR.off
@08:35:56.23 AFV.select :carrierSolution
@08:35:56.34 delay 2 seconds
@08:35:58.36 AS.seek 1ml
@08:36:56.10 delay 1 second
@08:36:57.12 AFV.select :holdingCoil
@08:36:57.23 delay 1 second
@08:36:58.25 AS.reconfigure ASfast
@08:36:58.30 AS.seek 0ml
@08:37:25.62 delay 3 seconds
@08:37:28.64 AFV.select :carrierSolution
@08:37:28.75 delay 2 seconds
@08:37:30.77 AS.reconfigure ASconfig
@08:37:30.82 AS.seek 1ml
@08:38:28.55 delay 1 second
@08:38:29.57 AFV.select :holdingCoil
@08:38:29.68 delay 1 second
@08:38:30.70 AS.reconfigure ASfast
@08:38:30.75 AS.seek 0ml
@08:38:58.07 delay 3 seconds
@08:39:01.09 AFV.select :carrierSolution
@08:39:01.20 delay 2 seconds
@08:39:03.22 AS.reconfigure ASconfig
@08:39:03.27 AS.seek 1ml
@08:40:01.01 delay 1 second
@08:40:02.03 AFV.select :holdingCoil
@08:40:02.14 delay 1 second
@08:40:03.16 AS.reconfigure ASfast
@08:40:03.21 AS.seek 0ml
@08:40:30.52 delay 3 seconds
@08:40:33.54 AFV.select :carrierSolution
@08:40:33.65 delay 2 seconds
@08:40:35.67 AS.reconfigure ASconfig
@08:40:35.72 AS.seek 1ml
@08:41:33.45 delay 1 second
@08:41:34.47 AFV.select :holdingCoil
@08:41:34.58 delay 1 second
@08:41:35.60 AS.reconfigure ASfast
@08:41:35.65 AS.seek 0ml
@08:42:02.98 delay 3 seconds
@08:42:06.00 AFV.select :carrierSolution
@08:42:06.11 delay 2 seconds
@08:42:08.13 AS.reconfigure ASconfig
@08:42:08.18 AS.seek 1ml
@08:43:05.93 delay 1 second
@08:43:06.95 AFV.select :holdingCoil
@08:43:07.06 delay 1 second
@08:43:08.08 AS.reconfigure ASnorm
@08:43:08.13 AS.seek 0ml
@08:43:58.82 <MAIN> delay 20 seconds
@08:44:18.99 <MFBPCR> Setting up PCR for AIPc -- writing /var/log/esp/AIPc21apr0708h.pcr
@08:44:19.01 Building PCR Slug from elutionWater with MM3 mix, PP6 primer
@08:44:19.02 Priming with carrier
@08:44:19.03 ATV.dial ARV
@08:44:19.65 ARV.dial :carrier
@08:44:19.99 AS.seek 0.5ml
@08:44:45.51 AFV.select :carrierSolution
@08:44:45.62 delay 2 seconds
@08:44:47.64 AS.reconfigure ASconfig
@08:44:47.68 AS.seek 1ml
@08:45:16.68 delay 1 second
@08:45:17.70 AFV.select :holdingCoil
@08:45:17.81 delay 1 second
@08:45:18.83 ATV.dial :waste
@08:45:20.56 AS.reconfigure ASnorm
@08:45:20.60 AS.seek 0ml
@08:46:11.29 AFV.select :carrierSolution
@08:46:11.40 delay 2 seconds
@08:46:13.42 AS.reconfigure ASconfig
@08:46:13.47 AS.seek 0.5ml
@08:46:42.48 delay 1 second
@08:46:43.50 AFV.select :holdingCoil
@08:46:43.61 Building Slug
@08:46:43.62 ATV.dial :ARV
@08:46:45.34 delay 2 seconds
@08:46:47.37 ARV.dial :air1
@08:46:47.75 delay 2 seconds
@08:46:49.82 AS.reconfigure ASslow2
@08:46:49.87 AS.seek 0.506ml
@08:46:56.18 delay 2 seconds
@08:46:58.21 ARV.dial :MM3
@08:46:59.29 delay 2 seconds
@08:47:01.32 Adding MM3
@08:47:01.38 AS.seek 0.524ml
@08:47:19.71 delay 25 seconds
@08:47:44.74 ARV.dial :PP6
@08:47:45.13 Adding PP6
@08:47:45.14 delay 2 seconds
@08:47:47.20 AS.seek 0.53ml
@08:47:53.43 delay 10 seconds
@08:48:03.47 ARV.dial :air1
@08:48:04.33 adding ARV air
@08:48:04.34 delay 2 seconds
@08:48:06.40 AS.seek 0.536ml
@08:48:12.66 delay 5 seconds
@08:48:17.69 ARV.dial :carrier
@08:48:18.07 delay 7 seconds
@08:48:25.14 AS.seek 0.594ml
@08:49:23.65 delay 5 seconds
@08:49:28.68 ATV.dial :elutionWater
@08:49:30.18 delay 2 seconds
@08:49:32.25 AS.seek 0.6ml
@08:49:38.65 delay 4 seconds
@08:49:42.68 ATV.dial ARV
@08:49:44.15 delay 2 seconds
@08:49:46.18 ARV.dial :carrier
@08:49:46.19 delay 2 seconds
@08:49:48.25 AS.seek 0.65ml
@08:50:39.09 delay 4 seconds
@08:50:43.12 ATV.dial PCR
@08:50:43.90 ASV.dial :waste1
@08:50:43.92 ARV.dial :air1
@08:50:44.32 Positioning Slug by 0.324ml
@08:50:44.39 AS.seek 0.326ml
@08:56:10.84 delay 5 seconds
@08:56:15.87 ATV.dial ARV
@08:56:16.65 ASV.dial :PCRlock
@08:56:17.04 delay 5 seconds
@08:56:22.07 Running AIPc ProfileIPc thermocycling
@08:56:22.08 <PCR> PCR.program ProfileIPc
@08:56:22.58 ProfileIPc should complete in < 1:26:35
@08:56:22.59 PCR.run 1:53:13.75
@10:05:26.46 PCR.off
@10:05:27.36 <MFBPCR> PCR for AIPc completed -- closed /var/log/esp/AIPc21apr0708h.pcr
@10:05:27.42 Cleaning PCR
@10:05:27.43 AFV.select :carrierSolution
@10:05:27.54 delay 2 seconds
@10:05:29.56 AS.reconfigure ASconfig
@10:05:29.61 AS.seek 0.5ml
@10:05:39.91 delay 1 second
@10:05:40.93 AFV.select :holdingCoil
@10:05:41.04 ASV.dial :waste1
@10:05:41.43 ATV.dial :air3
@10:05:42.76 AS.reconfigure ASslow1
@10:05:42.80 AS.seek 0.51ml
@10:05:45.07 delay 2 seconds
@10:05:47.10 ATV.dial :bleach
@10:05:47.72 AS.seek 0.81ml
@10:06:48.38 delay 3 seconds
@10:06:51.41 ATV.dial :air3
@10:06:51.98 delay 3 seconds
@10:06:55.05 AS.seek 0.835ml
@10:07:00.46 delay 3 seconds
@10:07:03.49 ATV.dial :bleach
@10:07:04.12 AS.seek 0.82ml
@10:07:07.35 delay 3 seconds
@10:07:10.38 ARV.dial :waste
@10:07:10.99 delay 3 seconds
@10:07:14.02 ATV.dial :ARV
@10:07:14.93 AS.reconfigure ASconfig
@10:07:14.97 AS.seek 0.47ml
@10:07:35.37 AFV.select :carrierSolution
@10:07:35.48 delay 2 seconds
@10:07:37.50 AS.seek 1ml
@10:08:08.27 delay 1 second
@10:08:09.29 AFV.select :holdingCoil
@10:08:09.40 delay 1 second
@10:08:10.42 AS.reconfigure ASfast
@10:08:10.47 AS.seek 0ml
@10:08:37.77 AFV.select :carrierSolution
@10:08:37.88 delay 2 seconds
@10:08:39.90 AS.reconfigure ASconfig
@10:08:39.95 AS.seek 1ml
@10:09:37.70 delay 1 second
@10:09:38.72 AFV.select :holdingCoil
@10:09:38.83 delay 1 second
@10:09:39.85 AS.reconfigure ASfast
@10:09:39.90 AS.seek 0ml
@10:10:07.24 AFV.select :carrierSolution
@10:10:07.35 delay 2 seconds
@10:10:09.37 AS.reconfigure ASconfig
@10:10:09.42 AS.seek 1ml
@10:11:07.16 delay 1 second
@10:11:08.18 AFV.select :holdingCoil
@10:11:08.29 delay 1 second
@10:11:09.31 AS.reconfigure ASfast
@10:11:09.36 AS.seek 0ml
@10:11:36.66 ARV.dial between carrier and air1
@10:11:37.19 ATV.dial :air3
@10:11:38.51 AS.reconfigure ASslow1
@10:11:38.55 AS.seek 0.01ml
@10:11:40.93 delay 2 seconds
@10:11:42.96 ATV.dial :bleach
@10:11:43.58 AS.seek 0.81ml
@10:14:24.71 delay 3 seconds
@10:14:27.74 ATV.dial :air3
@10:14:28.30 delay 3 seconds
@10:14:31.37 AS.seek 0.835ml
@10:14:36.78 delay 3 seconds
@10:14:39.81 ATV.dial :bleach
@10:14:40.44 AS.seek 0.82ml
@10:14:43.70 delay 3 seconds
@10:14:46.73 ATV.dial PCR
@10:14:47.13 AS.reconfigure ASconfig
@10:14:47.17 AS.seek 0ml
@10:15:34.57 PCR.program :bleachProfile
@10:15:34.64 PCR program should complete in < 2:45
@10:15:34.65 PCR.run 10:00
@10:18:30.82 PCR.off
@10:18:30.84 AFV.select :carrierSolution
@10:18:30.95 delay 2 seconds
@10:18:32.97 AS.seek 1ml
@10:19:30.69 delay 1 second
@10:19:31.71 AFV.select :holdingCoil
@10:19:31.82 delay 1 second
@10:19:32.84 AS.reconfigure ASfast
@10:19:32.89 AS.seek 0ml
@10:20:00.23 delay 3 seconds
@10:20:03.25 AFV.select :carrierSolution
@10:20:03.36 delay 2 seconds
@10:20:05.38 AS.reconfigure ASconfig
@10:20:05.43 AS.seek 1ml
@10:21:03.18 delay 1 second
@10:21:04.20 AFV.select :holdingCoil
@10:21:04.31 delay 1 second
@10:21:05.33 AS.reconfigure ASfast
@10:21:05.38 AS.seek 0ml
@10:21:32.71 delay 3 seconds
@10:21:35.73 AFV.select :carrierSolution
@10:21:35.84 delay 2 seconds
@10:21:37.86 AS.reconfigure ASconfig
@10:21:37.92 AS.seek 1ml
@10:22:35.68 delay 1 second
@10:22:36.70 AFV.select :holdingCoil
@10:22:36.81 delay 1 second
@10:22:37.83 AS.reconfigure ASfast
@10:22:37.88 AS.seek 0ml
@10:23:05.18 delay 3 seconds
@10:23:08.20 AFV.select :carrierSolution
@10:23:08.31 delay 2 seconds
@10:23:10.33 AS.reconfigure ASconfig
@10:23:10.38 AS.seek 1ml
@10:24:08.10 delay 1 second
@10:24:09.12 AFV.select :holdingCoil
@10:24:09.23 delay 1 second
@10:24:10.25 AS.reconfigure ASfast
@10:24:10.30 AS.seek 0ml
@10:24:37.62 delay 3 seconds
@10:24:40.64 AFV.select :carrierSolution
@10:24:40.75 delay 2 seconds
@10:24:42.77 AS.reconfigure ASconfig
@10:24:42.82 AS.seek 1ml
@10:25:40.60 delay 1 second
@10:25:41.62 AFV.select :holdingCoil
@10:25:41.73 delay 1 second
@10:25:42.75 AS.reconfigure ASnorm
@10:25:42.80 AS.seek 0ml
@11:31:52.39CEST08-Apr-21 <MAIN> ESP2 v3.30 I2C Gateway v3.06 for core bus
@11:31:54.47 
Configuration for mer 7/20/20 brent@mbari.org
/home/esp/esp2/type/shallow/mer/configure.rb
@11:31:54.55 
Configuration for MFB3 by preston@mbari.org
/home/esp/esp2/type/mfb/MFB3/configure.rb
@11:31:58.96 Polling Can every 10:00
@11:32:00.70 CS.configure :default
@11:32:00.73 CS.hold
@11:32:00.81 CS.configure :default
@11:32:00.89 PS.configure :default
@11:32:00.97 SS.configure :default
@11:32:01.01 SS.hold
@11:32:01.06 SS.configure :default
@11:32:01.14 CC.configure :default
@11:32:01.18 CC.hold
@11:32:01.25 CC.configure :default
@11:32:01.33 PC.configure :default
@11:32:01.37 PC.hold
@11:32:01.41 PC.configure :default
@11:32:01.51 Forearm.configure :loose
@11:32:01.55 Forearm.hold
@11:32:01.60 Forearm.configure :loose
@11:32:01.67 Elbow.configure :loose
@11:32:01.71 Elbow.hold
@11:32:01.77 Elbow.configure :loose
@11:32:01.87 SC.configure :loose
@11:32:01.91 SC.hold
@11:32:01.95 SC.configure :loose
@11:32:02.03 SE.configure :default
@11:32:02.07 SE.hold
@11:32:02.13 SE.configure :default
@11:32:02.17 CH.coast
@11:32:02.22 CH.configure :default
@11:32:02.24 PH.coast
@11:32:02.29 PH.configure :default
@11:32:02.39 SPE.coast
@11:32:02.44 SPE.configure SPEconfig
@11:32:02.51 AS.configure ASconfig
@11:32:02.55 AS.hold
@11:32:02.59 AS.configure ASconfig
@11:32:02.64 PCR.off
@11:32:02.68 PCR.pidtemp= 50,0.1,0,300,25,25
@11:32:02.71 PCR.caltemp= 2.5051,0.0372,0
@11:32:02.74 All dwarf controllers running firmware version 3.75
@11:32:02.94 Cleanly exited at 11:30:40CEST08-Apr-21
@11:32:02.97 Loading /home/esp/esp2/mission/GENOMAP.rb mission
@11:32:03.11 <MFBPCR> Extracting Nucleic Acid from extSrc
@11:32:03.14 SPE.hold 2:46:40,55C
@11:32:03.18 ASV.dial :SPEdelivery
@11:32:03.59 delay 20 seconds
@11:32:12.84 <bspe> Session Begun
@11:32:23.61 <MFBPCR> AFV.select :holdingCoil
@11:32:23.74 ATV.dial :air2
@11:32:25.00 AS.reconfigure ASslow1
@11:32:25.06 AS.seek 0.07ml
@11:32:39.47 delay 3 seconds
@11:32:42.50 ATV.dial :extSrc
@11:32:44.03 AS.seek 0.47ml
@11:34:04.81 delay 5 seconds
@11:34:09.89 Solid Phase Extraction Heater at 39.5C
@11:34:09.90 ATV.dial ASV
@11:34:10.78 ASV.dial :SPEdelivery
@11:34:10.87 AS.seek 0.042ml
@11:35:37.25 delay 10 seconds
@11:35:47.29 Rinsing external lysate line extSrc
@11:35:47.30 AFV.select :carrierSolution
@11:35:47.42 delay 2 seconds
@11:35:49.44 AS.reconfigure ASconfig
@11:35:49.50 AS.seek 1ml
@11:36:44.88 delay 1 second
@11:36:45.90 AFV.select :holdingCoil
@11:36:46.01 delay 1 second
@11:36:47.03 ATV.dial :extSrc
@11:36:47.87 AS.reconfigure ASnorm
@11:36:47.92 AS.seek 0ml
@11:37:38.64 ATV.dial :air2
@11:37:40.25 AS.reconfigure ASslow0
@11:37:40.31 AS.seek 0.05ml
@11:37:45.69 ATV.dial :extSrc
@11:37:47.28 AS.seek 0.01ml
@11:37:51.61 ATV.dial :waste
@11:37:53.01 AS.reconfigure ASconfig
@11:37:53.06 AS.seek 0ml
@11:37:54.28 ethanol wash
@11:37:54.29 ATV.dial :air2
@11:37:55.48 AS.reconfigure ASslow2
@11:37:55.54 AS.seek 0.01ml
@11:38:06.24 delay 3 seconds
@11:38:09.27 Pulling 0.4ml of Ethanol Wash
@11:38:09.28 ATV.dial :ethanol
@11:38:10.15 AS.reconfigure ASslow1
@11:38:10.21 AS.seek 0.41ml
@11:39:30.92 delay 3 seconds
@11:39:33.95 ATV.dial :air1
@11:39:34.38 delay 3 seconds
@11:39:37.46 AS.seek 0.435ml
@11:39:42.79 delay 3 seconds
@11:39:45.82 ATV.dial :ethanol
@11:39:46.45 AS.seek 0.42ml
@11:39:49.74 delay 3 seconds
@11:39:52.77 ATV.dial ASV
@11:39:54.17 AS.seek 0.093ml
@11:41:00.28 delay 3 seconds
@11:41:03.31 ATV.dial :waste2
@11:41:04.12 AS.reconfigure ASconfig
@11:41:04.17 AS.seek 0ml
@11:41:09.81 ATV.dial :air2
@11:41:10.23 AS.reconfigure ASslow1
@11:41:10.28 AS.seek 1ml
@11:44:31.67 delay 5 seconds
@11:44:36.70 ATV.dial ASV
@11:44:37.74 ASV.dial :SPEdelivery
@11:44:37.77 AS.seek 0.02ml
@11:47:55.16 delay 5 seconds
@11:48:00.19 ATV.dial :air2
@11:48:01.28 AS.seek 0.52ml
@11:49:42.13 delay 5 seconds
@11:49:47.16 ATV.dial ASV
@11:49:48.20 ASV.dial :wasteR
@11:49:48.59 AS.seek 0.3ml
@11:50:33.13 delay 20 seconds
@11:50:53.17 AS.seek 0.07ml
@11:51:39.69 delay 20 seconds
@11:51:59.72 ATV.dial :waste2
@11:52:00.53 AS.reconfigure ASconfig
@11:52:00.58 AS.seek 0ml
@11:52:04.91 Elution
@11:52:04.92 ATV.dial :air1
@11:52:06.12 AS.reconfigure ASslow1
@11:52:06.18 AS.seek 0.05ml
@11:52:16.55 delay 3 seconds
@11:52:19.58 ATV.dial :ElutionWater
@11:52:20.30 AS.seek 0.11ml
@11:52:32.64 delay 3 seconds
@11:52:35.67 ATV.dial ASV,via: :air2
@11:52:37.90 ASV.dial :SPEelution
@11:52:38.52 AFV.select :carrierSolution
@11:52:38.68 AS.reconfigure ASnorm
@11:52:38.74 AS.seek 0.76ml
@11:53:11.77 delay 3 seconds
@11:53:14.79 AFV.select :holdingCoil
@11:53:14.94 AS.reconfigure ASslow1
@11:53:15.00 AS.seek 0.475ml
@11:54:12.58 delay 5 seconds
@11:54:17.66 AS.reconfigure ASslow2
@11:54:17.72 AS.seek 0.44ml
@11:54:53.03 delay 1:00
@11:55:53.07 pushing eluate to holding coil
@11:55:53.14 AS.seek 0.28ml
@11:58:34.34 delay 30 seconds
@11:59:04.38 ATV.dial :waste,via: :air3
@11:59:06.20 AS.reconfigure ASconfig
@11:59:06.25 AS.seek 0ml
@11:59:22.66 Parking Bleach in LysateSource line and SPEcolumn
@11:59:22.67 ATV.dial :air3
@11:59:23.58 AS.reconfigure ASslow1
@11:59:23.64 AS.seek 0.03ml
@11:59:29.97 ATV.dial :ElutionWater
@11:59:30.62 AS.seek 0.06ml
@11:59:37.02 ATV.dial :air3
@11:59:37.65 AS.seek 0.07ml
@11:59:40.05 delay 2 seconds
@11:59:42.08 ATV.dial :bleach
@11:59:42.58 AS.seek 0.15ml
@11:59:58.97 delay 3 seconds
@12:00:02.00 ATV.dial :air3
@12:00:02.59 delay 3 seconds
@12:00:05.66 AS.seek 0.175ml
@12:00:11.06 delay 3 seconds
@12:00:14.09 ATV.dial :bleach
@12:00:14.73 AS.seek 0.16ml
@12:00:18.02 delay 3 seconds
@12:00:21.05 ATV.dial :extSrc
@12:00:21.70 AS.reconfigure ASconfig
@12:00:21.75 AS.seek 0.015ml
@12:00:30.41 ATV.dial :waste
@12:00:31.95 AS.seek 0ml
@12:00:33.09 ATV.dial :air3
@12:00:33.97 AS.reconfigure ASslow1
@12:00:34.03 AS.seek 0.01ml
@12:00:36.42 delay 2 seconds
@12:00:38.45 ATV.dial :bleach
@12:00:38.92 AS.seek 0.58ml
@12:02:33.84 delay 3 seconds
@12:02:36.86 ATV.dial :air3
@12:02:37.44 delay 3 seconds
@12:02:40.52 AS.seek 0.605ml
@12:02:45.92 delay 3 seconds
@12:02:48.95 ATV.dial :bleach
@12:02:49.58 AS.seek 0.59ml
@12:02:52.88 delay 3 seconds
@12:02:55.91 ATV.dial :ASV
@12:02:57.00 ASV.dial :wasteR
@12:02:57.64 AS.seek 0ml
@12:04:56.55 delay 1:00
@12:05:56.58 AFV.select :carrierSolution
@12:05:56.69 delay 2 seconds
@12:05:58.71 AS.reconfigure ASconfig
@12:05:58.76 AS.seek 1ml
@12:06:56.53 delay 1 second
@12:06:57.55 AFV.select :holdingCoil
@12:06:57.66 delay 1 second
@12:06:58.68 ATV.dial ASV
@12:06:58.69 ASV.dial :wasteR
@12:06:58.71 AS.reconfigure ASslow1
@12:06:58.77 AS.seek 0ml
@12:10:20.16 delay 20 seconds
@12:10:40.20 ATV.dial :air3
@12:10:41.78 AS.seek 0.59ml
@12:12:40.74 delay 3 seconds
@12:12:43.77 ATV.dial :ASV
@12:12:45.28 ASV.dial :wasteR
@12:12:45.37 AS.seek 0.09ml
@12:14:26.22 delay 30 seconds
@12:14:56.26 ATV.dial :extSrc
@12:14:57.08 delay 3 seconds
@12:15:00.11 ATV.dial :ElutionWater
@12:15:01.29 AS.seek 0.12ml
@12:15:07.61 ATV.dial :air3
@12:15:08.25 AS.seek 0.13ml
@12:15:10.55 delay 2 seconds
@12:15:12.58 ATV.dial :bleach
@12:15:13.07 AS.seek 0.23ml
@12:15:33.58 delay 3 seconds
@12:15:36.61 ATV.dial :air3
@12:15:37.19 delay 3 seconds
@12:15:40.26 AS.seek 0.255ml
@12:15:45.67 delay 3 seconds
@12:15:48.70 ATV.dial :bleach
@12:15:49.78 AS.seek 0.24ml
@12:15:53.05 delay 3 seconds
@12:15:56.08 ATV.dial :ASV
@12:15:57.17 ASV.dial :SPEdelivery
@12:15:57.56 AFV.select :carrierSolution
@12:15:57.68 delay 2 seconds
@12:15:59.70 AS.reconfigure ASconfig
@12:15:59.75 AS.seek 0.5ml
@12:16:15.00 delay 1 second
@12:16:16.02 AFV.select :holdingCoil
@12:16:16.17 AS.reconfigure ASslow1
@12:16:16.23 AS.seek 0.17ml
@12:17:22.90 SPE.hold 2:46:40,95C
@12:17:22.96 delay 3:00
@12:20:23.00 AFV.select :carrierSolution
@12:20:23.12 delay 2 seconds
@12:20:25.14 AS.reconfigure ASconfig
@12:20:25.19 AS.seek 1ml
@12:21:13.19 delay 1 second
@12:21:14.21 AFV.select :holdingCoil
@12:21:14.32 delay 1 second
@12:21:15.34 ASV.dial :wasteD
@12:21:16.54 AS.seek 0ml
@12:22:14.30 Priming Eluate
@12:22:14.31 AFV.select :carrierSolution
@12:22:14.44 delay 2 seconds
@12:22:16.46 AS.seek 0.5ml
@12:22:45.48 delay 1 second
@12:22:46.50 AFV.select :holdingCoil
@12:22:46.61 ATV.dial ASV
@12:22:46.62 ASV.dial :SPErecovery
@12:22:47.25 Swish #1 of 2
@12:22:47.34 AS.reconfigure ASslow0
@12:22:47.39 AS.seek 0.6ml
@12:22:57.84 AS.seek 0.5ml
@12:23:08.27 Swish #2 of 2
@12:23:08.36 AS.seek 0.6ml
@12:23:18.83 AS.seek 0.5ml
@12:23:29.23 delay 3 seconds
@12:23:32.30 AS.reconfigure ASslow1
@12:23:32.36 AS.seek 0.684ml
@12:24:09.67 delay 3 seconds
@12:24:12.70 Eluate primed
@12:24:12.71 ATV.dial :waste2
@12:24:13.40 AS.reconfigure ASnorm
@12:24:13.45 AS.seek 0ml
@12:24:48.25 SPE.coast
@12:24:48.33 ASV.dial :SPErecovery
@12:24:48.35 Setting up PCR for ACYPP2 -- writing /var/log/esp/ACYPP221apr0812h.pcr
@12:24:48.38 Building PCR Slug from ASV with MM1 mix, PP2 primer
@12:24:48.40 Priming with carrier
@12:24:48.41 ATV.dial ARV
@12:24:49.47 ARV.dial :carrier
@12:24:49.84 AS.seek 0.5ml
@12:25:15.36 AFV.select :carrierSolution
@12:25:15.48 delay 2 seconds
@12:25:17.50 AS.reconfigure ASconfig
@12:25:17.56 AS.seek 1ml
@12:25:46.56 delay 1 second
@12:25:47.58 AFV.select :holdingCoil
@12:25:47.69 delay 1 second
@12:25:48.71 ATV.dial :waste
@12:25:50.32 AS.reconfigure ASnorm
@12:25:50.38 AS.seek 0ml
@12:26:41.09 AFV.select :carrierSolution
@12:26:41.21 delay 2 seconds
@12:26:43.23 AS.reconfigure ASconfig
@12:26:43.29 AS.seek 0.5ml
@12:27:12.33 delay 1 second
@12:27:13.35 AFV.select :holdingCoil
@12:27:13.46 Building Slug
@12:27:13.47 ATV.dial :ARV
@12:27:15.23 delay 2 seconds
@12:27:17.25 ARV.dial :air1
@12:27:17.67 delay 2 seconds
@12:27:19.74 AS.reconfigure ASslow2
@12:27:19.80 AS.seek 0.506ml
@12:27:26.02 delay 2 seconds
@12:27:28.05 ARV.dial :MM1
@12:27:28.69 delay 2 seconds
@12:27:30.72 Adding MM1
@12:27:30.79 AS.seek 0.524ml
@12:27:49.28 delay 25 seconds
@12:28:14.31 ARV.dial :PP2
@12:28:14.73 Adding PP2
@12:28:14.74 delay 2 seconds
@12:28:16.82 AS.seek 0.53ml
@12:28:23.22 delay 10 seconds
@12:28:33.26 ARV.dial :air2
@12:28:33.68 adding ARV air
@12:28:33.69 delay 2 seconds
@12:28:35.77 AS.seek 0.536ml
@12:28:41.91 delay 5 seconds
@12:28:46.94 ARV.dial :carrier
@12:28:48.30 delay 7 seconds
@12:28:55.39 AS.seek 0.594ml
@12:29:53.84 delay 5 seconds
@12:29:58.87 ATV.dial :ASV
@12:29:59.30 delay 2 seconds
@12:30:01.38 AS.seek 0.6ml
@12:30:07.80 delay 4 seconds
@12:30:11.83 ATV.dial ARV
@12:30:12.39 delay 2 seconds
@12:30:14.42 ARV.dial :carrier
@12:30:14.43 delay 2 seconds
@12:30:16.51 AS.seek 0.65ml
@12:31:07.16 delay 4 seconds
@12:31:11.19 ATV.dial PCR
@12:31:11.84 ASV.dial :waste1
@12:31:12.76 ARV.dial :air1
@12:31:13.18 Positioning Slug by 0.324ml
@12:31:13.26 AS.seek 0.326ml
@12:36:39.48 delay 5 seconds
@12:36:44.50 ATV.dial ARV
@12:36:45.31 ASV.dial :PCRlock
@12:36:45.70 delay 5 seconds
@12:36:50.73 Running ACYPP2 Profile60CIQ2 thermocycling
@12:36:50.74 <PCR> PCR.program Profile60CIQ2
@12:36:50.86 Profile60CIQ2 should complete in < 1:47:50
@12:36:50.87 PCR.run 2:19:47.5
@14:05:41.47 PCR.off
@14:05:42.11 <MFBPCR> Running ACYPP2 MeltProfile60 thermocycling
@14:05:42.12 <PCR> PCR.program MeltProfile60
@14:05:42.23 MeltProfile60 should complete in < 11:55
@14:05:42.24 PCR.run 19:53.75
@14:13:26.36 PCR.off
@14:13:27.31 <MFBPCR> PCR for ACYPP2 completed -- closed /var/log/esp/ACYPP221apr0812h.pcr
@14:13:27.39 Cleaning PCR
@14:13:27.40 AFV.select :carrierSolution
@14:13:27.52 delay 2 seconds
@14:13:29.54 AS.reconfigure ASconfig
@14:13:29.60 AS.seek 0.5ml
@14:13:39.89 delay 1 second
@14:13:40.91 AFV.select :holdingCoil
@14:13:41.02 ASV.dial :waste1
@14:13:41.41 ATV.dial :air3
@14:13:42.76 AS.reconfigure ASslow1
@14:13:42.82 AS.seek 0.51ml
@14:13:45.22 delay 2 seconds
@14:13:47.25 ATV.dial :bleach
@14:13:47.90 AS.seek 0.81ml
@14:14:48.50 delay 3 seconds
@14:14:51.53 ATV.dial :air3
@14:14:52.11 delay 3 seconds
@14:14:55.18 AS.seek 0.835ml
@14:15:00.51 delay 3 seconds
@14:15:03.54 ATV.dial :bleach
@14:15:04.18 AS.seek 0.82ml
@14:15:07.48 delay 3 seconds
@14:15:10.51 ARV.dial :waste
@14:15:11.16 delay 3 seconds
@14:15:14.19 ATV.dial :ARV
@14:15:15.11 AS.reconfigure ASconfig
@14:15:15.17 AS.seek 0.47ml
@14:15:35.58 AFV.select :carrierSolution
@14:15:35.69 delay 2 seconds
@14:15:37.71 AS.seek 1ml
@14:16:08.47 delay 1 second
@14:16:09.49 AFV.select :holdingCoil
@14:16:09.60 delay 1 second
@14:16:10.62 AS.reconfigure ASfast
@14:16:10.68 AS.seek 0ml
@14:16:38.03 AFV.select :carrierSolution
@14:16:38.15 delay 2 seconds
@14:16:40.17 AS.reconfigure ASconfig
@14:16:40.22 AS.seek 1ml
@14:17:37.98 delay 1 second
@14:17:39.00 AFV.select :holdingCoil
@14:17:39.11 delay 1 second
@14:17:40.13 AS.reconfigure ASfast
@14:17:40.19 AS.seek 0ml
@14:18:07.51 AFV.select :carrierSolution
@14:18:07.63 delay 2 seconds
@14:18:09.65 AS.reconfigure ASconfig
@14:18:09.70 AS.seek 1ml
@14:19:07.44 delay 1 second
@14:19:08.46 AFV.select :holdingCoil
@14:19:08.57 delay 1 second
@14:19:09.59 AS.reconfigure ASfast
@14:19:09.65 AS.seek 0ml
@14:19:37.00 ARV.dial between carrier and air1
@14:19:37.54 ATV.dial :air3
@14:19:38.89 AS.reconfigure ASslow1
@14:19:38.95 AS.seek 0.01ml
@14:19:41.26 delay 2 seconds
@14:19:43.29 ATV.dial :bleach
@14:19:43.95 AS.seek 0.81ml
@14:22:25.17 delay 3 seconds
@14:22:28.20 ATV.dial :air3
@14:22:28.78 delay 3 seconds
@14:22:31.85 AS.seek 0.835ml
@14:22:37.17 delay 3 seconds
@14:22:40.20 ATV.dial :bleach
@14:22:40.84 AS.seek 0.82ml
@14:22:44.14 delay 3 seconds
@14:22:47.17 ATV.dial PCR
@14:22:47.60 AS.reconfigure ASconfig
@14:22:47.65 AS.seek 0ml
@14:23:35.05 PCR.program :bleachProfile
@14:23:35.12 PCR program should complete in < 2:45
@14:23:35.14 PCR.run 10:00
@14:26:31.33 PCR.off
@14:26:31.38 AFV.select :carrierSolution
@14:26:31.49 delay 2 seconds
@14:26:33.51 AS.seek 1ml
@14:27:31.30 delay 1 second
@14:27:32.32 AFV.select :holdingCoil
@14:27:32.43 delay 1 second
@14:27:33.45 AS.reconfigure ASfast
@14:27:33.51 AS.seek 0ml
@14:28:00.84 delay 3 seconds
@14:28:03.86 AFV.select :carrierSolution
@14:28:03.97 delay 2 seconds
@14:28:05.99 AS.reconfigure ASconfig
@14:28:06.06 AS.seek 1ml
@14:29:03.80 delay 1 second
@14:29:04.82 AFV.select :holdingCoil
@14:29:04.93 delay 1 second
@14:29:05.95 AS.reconfigure ASfast
@14:29:06.01 AS.seek 0ml
@14:29:33.34 delay 3 seconds
@14:29:36.36 AFV.select :carrierSolution
@14:29:36.47 delay 2 seconds
@14:29:38.49 AS.reconfigure ASconfig
@14:29:38.55 AS.seek 1ml
@14:30:36.31 delay 1 second
@14:30:37.33 AFV.select :holdingCoil
@14:30:37.44 delay 1 second
@14:30:38.46 AS.reconfigure ASfast
@14:30:38.52 AS.seek 0ml
@14:31:05.86 delay 3 seconds
@14:31:08.88 AFV.select :carrierSolution
@14:31:08.99 delay 2 seconds
@14:31:11.01 AS.reconfigure ASconfig
@14:31:11.08 AS.seek 1ml
@14:32:08.83 delay 1 second
@14:32:09.85 AFV.select :holdingCoil
@14:32:09.96 delay 1 second
@14:32:10.98 AS.reconfigure ASfast
@14:32:11.04 AS.seek 0ml
@14:32:38.37 delay 3 seconds
@14:32:41.39 AFV.select :carrierSolution
@14:32:41.50 delay 2 seconds
@14:32:43.52 AS.reconfigure ASconfig
@14:32:43.58 AS.seek 1ml
@14:33:41.33 delay 1 second
@14:33:42.35 AFV.select :holdingCoil
@14:33:42.46 delay 1 second
@14:33:43.48 AS.reconfigure ASnorm
@14:33:43.53 AS.seek 0ml
@14:34:34.25 Setting up PCR for AOPP1 -- writing /var/log/esp/AOPP121apr0814h.pcr
@14:34:34.26 Building PCR Slug from ASV with MM1 mix, PP1 primer
@14:34:34.29 Priming with carrier
@14:34:34.30 ATV.dial ARV
@14:34:35.06 ARV.dial :carrier
@14:34:35.42 AS.seek 0.5ml
@14:35:01.03 AFV.select :carrierSolution
@14:35:01.16 delay 2 seconds
@14:35:03.18 AS.reconfigure ASconfig
@14:35:03.24 AS.seek 1ml
@14:35:32.26 delay 1 second
@14:35:33.28 AFV.select :holdingCoil
@14:35:33.39 delay 1 second
@14:35:34.41 ATV.dial :waste
@14:35:36.17 AS.reconfigure ASnorm
@14:35:36.23 AS.seek 0ml
@14:36:26.92 AFV.select :carrierSolution
@14:36:27.05 delay 2 seconds
@14:36:29.07 AS.reconfigure ASconfig
@14:36:29.13 AS.seek 0.5ml
@14:36:58.16 delay 1 second
@14:36:59.18 AFV.select :holdingCoil
@14:36:59.29 Building Slug
@14:36:59.30 ATV.dial :ARV
@14:37:01.04 delay 2 seconds
@14:37:03.06 ARV.dial :air1
@14:37:03.48 delay 2 seconds
@14:37:05.55 AS.reconfigure ASslow2
@14:37:05.61 AS.seek 0.506ml
@14:37:11.98 delay 2 seconds
@14:37:14.01 ARV.dial :MM1
@14:37:14.64 delay 2 seconds
@14:37:16.67 Adding MM1
@14:37:16.74 AS.seek 0.524ml
@14:37:35.23 delay 25 seconds
@14:38:00.26 ARV.dial :PP1
@14:38:00.67 Adding PP1
@14:38:00.68 delay 2 seconds
@14:38:02.76 AS.seek 0.53ml
@14:38:09.12 delay 10 seconds
@14:38:19.16 ARV.dial :air1
@14:38:19.57 adding ARV air
@14:38:19.58 delay 2 seconds
@14:38:21.66 AS.seek 0.536ml
@14:38:28.22 delay 5 seconds
@14:38:33.25 ARV.dial :carrier
@14:38:33.67 delay 7 seconds
@14:38:40.76 AS.seek 0.594ml
@14:39:39.61 delay 5 seconds
@14:39:44.64 ATV.dial :ASV
@14:39:45.06 delay 2 seconds
@14:39:47.13 AS.seek 0.6ml
@14:39:53.48 delay 4 seconds
@14:39:57.51 ATV.dial ARV
@14:39:58.08 delay 2 seconds
@14:40:00.11 ARV.dial :carrier
@14:40:00.12 delay 2 seconds
@14:40:02.20 AS.seek 0.65ml
@14:40:53.03 delay 4 seconds
@14:40:57.06 ATV.dial PCR
@14:40:57.70 ASV.dial :waste1
@14:40:57.72 ARV.dial :air1
@14:40:58.14 Positioning Slug by 0.324ml
@14:40:58.22 AS.seek 0.326ml
@14:46:24.56 delay 5 seconds
@14:46:29.59 ATV.dial ARV
@14:46:30.41 ASV.dial :PCRlock
@14:46:30.82 delay 5 seconds
@14:46:35.84 Running AOPP1 Profile60CIQ1 thermocycling
@14:46:35.85 <PCR> PCR.program Profile60CIQ1
@14:46:35.97 Profile60CIQ1 should complete in < 1:56:15
@14:46:35.98 PCR.run 2:30:18.75
@16:25:53.39 PCR.off
@16:25:53.96 <MFBPCR> Running AOPP1 MeltProfile60 thermocycling
@16:25:53.97 <PCR> PCR.program MeltProfile60
@16:25:54.46 MeltProfile60 should complete in < 11:55
@16:25:54.47 PCR.run 19:53.75
@16:33:35.48 PCR.off
@16:33:36.42 <MFBPCR> PCR for AOPP1 completed -- closed /var/log/esp/AOPP121apr0814h.pcr
@16:33:36.50 Cleaning PCR
@16:33:36.51 AFV.select :carrierSolution
@16:33:36.63 delay 2 seconds
@16:33:38.65 AS.reconfigure ASconfig
@16:33:38.71 AS.seek 0.5ml
@16:33:49.01 delay 1 second
@16:33:50.03 AFV.select :holdingCoil
@16:33:50.14 ASV.dial :waste1
@16:33:50.53 ATV.dial :air3
@16:33:51.89 AS.reconfigure ASslow1
@16:33:51.95 AS.seek 0.51ml
@16:33:54.22 delay 2 seconds
@16:33:56.25 ATV.dial :bleach
@16:33:56.90 AS.seek 0.81ml
@16:34:57.55 delay 3 seconds
@16:35:00.58 ATV.dial :air3
@16:35:01.16 delay 3 seconds
@16:35:04.23 AS.seek 0.835ml
@16:35:09.66 delay 3 seconds
@16:35:12.69 ATV.dial :bleach
@16:35:13.32 AS.seek 0.82ml
@16:35:16.72 delay 3 seconds
@16:35:19.75 ARV.dial :waste
@16:35:20.37 delay 3 seconds
@16:35:23.40 ATV.dial :ARV
@16:35:24.32 AS.reconfigure ASconfig
@16:35:24.38 AS.seek 0.47ml
@16:35:44.81 AFV.select :carrierSolution
@16:35:44.93 delay 2 seconds
@16:35:46.95 AS.seek 1ml
@16:36:17.72 delay 1 second
@16:36:18.74 AFV.select :holdingCoil
@16:36:18.85 delay 1 second
@16:36:19.87 AS.reconfigure ASfast
@16:36:19.93 AS.seek 0ml
@16:36:47.25 AFV.select :carrierSolution
@16:36:47.36 delay 2 seconds
@16:36:49.38 AS.reconfigure ASconfig
@16:36:49.43 AS.seek 1ml
@16:37:47.17 delay 1 second
@16:37:48.19 AFV.select :holdingCoil
@16:37:48.30 delay 1 second
@16:37:49.32 AS.reconfigure ASfast
@16:37:49.38 AS.seek 0ml
@16:38:16.72 AFV.select :carrierSolution
@16:38:16.84 delay 2 seconds
@16:38:18.86 AS.reconfigure ASconfig
@16:38:18.91 AS.seek 1ml
@16:39:16.67 delay 1 second
@16:39:17.69 AFV.select :holdingCoil
@16:39:17.80 delay 1 second
@16:39:18.82 AS.reconfigure ASfast
@16:39:18.88 AS.seek 0ml
@16:39:46.20 ARV.dial between carrier and air1
@16:39:46.73 ATV.dial :air3
@16:39:48.07 AS.reconfigure ASslow1
@16:39:48.13 AS.seek 0.01ml
@16:39:50.44 delay 2 seconds
@16:39:52.47 ATV.dial :bleach
@16:39:53.11 AS.seek 0.81ml
@16:42:34.26 delay 3 seconds
@16:42:37.29 ATV.dial :air3
@16:42:37.87 delay 3 seconds
@16:42:40.94 AS.seek 0.835ml
@16:42:46.22 delay 3 seconds
@16:42:49.25 ATV.dial :bleach
@16:42:49.89 AS.seek 0.82ml
@16:42:53.34 delay 3 seconds
@16:42:56.37 ATV.dial PCR
@16:42:56.79 AS.reconfigure ASconfig
@16:42:56.84 AS.seek 0ml
@16:43:44.28 PCR.program :bleachProfile
@16:43:44.35 PCR program should complete in < 2:45
@16:43:44.37 PCR.run 10:00
@16:46:40.58 PCR.off
@16:46:40.62 AFV.select :carrierSolution
@16:46:40.73 delay 2 seconds
@16:46:42.75 AS.seek 1ml
@16:47:40.55 delay 1 second
@16:47:41.57 AFV.select :holdingCoil
@16:47:41.68 delay 1 second
@16:47:42.70 AS.reconfigure ASfast
@16:47:42.76 AS.seek 0ml
@16:48:10.09 delay 3 seconds
@16:48:13.11 AFV.select :carrierSolution
@16:48:13.22 delay 2 seconds
@16:48:15.24 AS.reconfigure ASconfig
@16:48:15.30 AS.seek 1ml
@16:49:13.06 delay 1 second
@16:49:14.08 AFV.select :holdingCoil
@16:49:14.19 delay 1 second
@16:49:15.21 AS.reconfigure ASfast
@16:49:15.27 AS.seek 0ml
@16:49:42.61 delay 3 seconds
@16:49:45.63 AFV.select :carrierSolution
@16:49:45.76 delay 2 seconds
@16:49:47.78 AS.reconfigure ASconfig
@16:49:47.84 AS.seek 1ml
@16:50:45.59 delay 1 second
@16:50:46.61 AFV.select :holdingCoil
@16:50:46.72 delay 1 second
@16:50:47.74 AS.reconfigure ASfast
@16:50:47.80 AS.seek 0ml
@16:51:15.14 delay 3 seconds
@16:51:18.16 AFV.select :carrierSolution
@16:51:18.27 delay 2 seconds
@16:51:20.29 AS.reconfigure ASconfig
@16:51:20.35 AS.seek 1ml
@16:52:18.12 delay 1 second
@16:52:19.14 AFV.select :holdingCoil
@16:52:19.25 delay 1 second
@16:52:20.27 AS.reconfigure ASfast
@16:52:20.33 AS.seek 0ml
@16:52:47.66 delay 3 seconds
@16:52:50.68 AFV.select :carrierSolution
@16:52:50.79 delay 2 seconds
@16:52:52.81 AS.reconfigure ASconfig
@16:52:52.87 AS.seek 1ml
@16:53:50.65 delay 1 second
@16:53:51.67 AFV.select :holdingCoil
@16:53:51.78 delay 1 second
@16:53:52.80 AS.reconfigure ASnorm
@16:53:52.86 AS.seek 0ml
@16:54:43.59 Setting up PCR for AARPP4 -- writing /var/log/esp/AARPP421apr0816h.pcr
@16:54:43.61 Building PCR Slug from ASV with MM2 mix, PP4 primer
@16:54:43.63 Priming with carrier
@16:54:43.65 ATV.dial ARV
@16:54:44.28 ARV.dial :carrier
@16:54:44.64 AS.seek 0.5ml
@16:55:10.17 AFV.select :carrierSolution
@16:55:10.30 delay 2 seconds
@16:55:12.32 AS.reconfigure ASconfig
@16:55:12.38 AS.seek 1ml
@16:55:41.40 delay 1 second
@16:55:42.42 AFV.select :holdingCoil
@16:55:42.53 delay 1 second
@16:55:43.55 ATV.dial :waste
@16:55:45.32 AS.reconfigure ASnorm
@16:55:45.38 AS.seek 0ml
@16:56:36.08 AFV.select :carrierSolution
@16:56:36.21 delay 2 seconds
@16:56:38.23 AS.reconfigure ASconfig
@16:56:38.29 AS.seek 0.5ml
@16:57:07.36 delay 1 second
@16:57:08.38 AFV.select :holdingCoil
@16:57:08.49 Building Slug
@16:57:08.50 ATV.dial :ARV
@16:57:10.25 delay 2 seconds
@16:57:12.28 ARV.dial :air1
@16:57:12.68 delay 2 seconds
@16:57:14.75 AS.reconfigure ASslow2
@16:57:14.81 AS.seek 0.506ml
@16:57:21.03 delay 2 seconds
@16:57:23.06 ARV.dial :MM2
@16:57:25.06 delay 2 seconds
@16:57:27.09 Adding MM2
@16:57:27.16 AS.seek 0.524ml
@16:57:45.75 delay 25 seconds
@16:58:10.78 ARV.dial :PP4
@16:58:11.20 Adding PP4
@16:58:11.21 delay 2 seconds
@16:58:13.29 AS.seek 0.53ml
@16:58:19.61 delay 10 seconds
@16:58:29.65 ARV.dial :air3
@16:58:30.07 adding ARV air
@16:58:30.08 delay 2 seconds
@16:58:32.15 AS.seek 0.536ml
@16:58:38.53 delay 5 seconds
@16:58:43.56 ARV.dial :carrier
@16:58:44.89 delay 7 seconds
@16:58:51.98 AS.seek 0.594ml
@16:59:50.58 delay 5 seconds
@16:59:55.61 ATV.dial :ASV
@16:59:56.03 delay 2 seconds
@16:59:58.10 AS.seek 0.6ml
@17:00:04.75 delay 4 seconds
@17:00:08.78 ATV.dial ARV
@17:00:09.34 delay 2 seconds
@17:00:11.37 ARV.dial :carrier
@17:00:11.38 delay 2 seconds
@17:00:13.45 AS.seek 0.65ml
@17:01:04.53 delay 4 seconds
@17:01:08.56 ATV.dial PCR
@17:01:09.20 ASV.dial :waste1
@17:01:09.22 ARV.dial :air1
@17:01:09.63 Positioning Slug by 0.324ml
@17:01:09.72 AS.seek 0.326ml
@17:06:36.05 delay 5 seconds
@17:06:41.08 ATV.dial ARV
@17:06:41.89 ASV.dial :PCRlock
@17:06:42.30 delay 5 seconds
@17:06:47.33 Running AARPP4 Profile58CIQ2 thermocycling
@17:06:47.34 <PCR> PCR.program Profile58CIQ2
@17:06:47.46 Profile58CIQ2 should complete in < 1:36:15
@17:06:47.47 PCR.run 2:05:18.75
@18:27:41.57 PCR.off
@18:27:42.13 <MFBPCR> Running AARPP4 MeltProfile58 thermocycling
@18:27:42.15 <PCR> PCR.program MeltProfile58
@18:27:42.26 MeltProfile58 should complete in < 12:33
@18:27:42.27 PCR.run 20:41.25
@18:35:30.36 PCR.off
@18:35:31.35 <MFBPCR> PCR for AARPP4 completed -- closed /var/log/esp/AARPP421apr0816h.pcr
@18:35:31.42 Cleaning PCR
@18:35:31.43 AFV.select :carrierSolution
@18:35:31.56 delay 2 seconds
@18:35:33.58 AS.reconfigure ASconfig
@18:35:33.63 AS.seek 0.5ml
@18:35:43.94 delay 1 second
@18:35:44.96 AFV.select :holdingCoil
@18:35:45.07 ASV.dial :waste1
@18:35:45.47 ATV.dial :air3
@18:35:46.84 AS.reconfigure ASslow1
@18:35:46.90 AS.seek 0.51ml
@18:35:49.60 delay 2 seconds
@18:35:51.63 ATV.dial :bleach
@18:35:52.28 AS.seek 0.81ml
@18:36:52.89 delay 3 seconds
@18:36:55.92 ATV.dial :air3
@18:36:56.50 delay 3 seconds
@18:36:59.57 AS.seek 0.835ml
@18:37:04.91 delay 3 seconds
@18:37:07.94 ATV.dial :bleach
@18:37:08.59 AS.seek 0.82ml
@18:37:11.87 delay 3 seconds
@18:37:14.90 ARV.dial :waste
@18:37:15.53 delay 3 seconds
@18:37:18.56 ATV.dial :ARV
@18:37:19.48 AS.reconfigure ASconfig
@18:37:19.53 AS.seek 0.47ml
@18:37:39.94 AFV.select :carrierSolution
@18:37:40.06 delay 2 seconds
@18:37:42.08 AS.seek 1ml
@18:38:12.81 delay 1 second
@18:38:13.83 AFV.select :holdingCoil
@18:38:13.94 delay 1 second
@18:38:14.96 AS.reconfigure ASfast
@18:38:15.02 AS.seek 0ml
@18:38:42.37 AFV.select :carrierSolution
@18:38:42.49 delay 2 seconds
@18:38:44.51 AS.reconfigure ASconfig
@18:38:44.57 AS.seek 1ml
@18:39:42.34 delay 1 second
@18:39:43.36 AFV.select :holdingCoil
@18:39:43.47 delay 1 second
@18:39:44.49 AS.reconfigure ASfast
@18:39:44.55 AS.seek 0ml
@18:40:11.90 AFV.select :carrierSolution
@18:40:12.41 delay 2 seconds
@18:40:14.45 AS.reconfigure ASconfig
@18:40:14.50 AS.seek 1ml
@18:41:12.26 delay 1 second
@18:41:13.28 AFV.select :holdingCoil
@18:41:13.39 delay 1 second
@18:41:14.41 AS.reconfigure ASfast
@18:41:14.47 AS.seek 0ml
@18:41:41.83 ARV.dial between carrier and air1
@18:41:42.37 ATV.dial :air3
@18:41:43.71 AS.reconfigure ASslow1
@18:41:43.77 AS.seek 0.01ml
@18:41:46.26 delay 2 seconds
@18:41:48.29 ATV.dial :bleach
@18:41:48.95 AS.seek 0.81ml
@18:44:30.20 delay 3 seconds
@18:44:33.23 ATV.dial :air3
@18:44:33.81 delay 3 seconds
@18:44:36.88 AS.seek 0.835ml
@18:44:42.19 delay 3 seconds
@18:44:45.22 ATV.dial :bleach
@18:44:45.84 AS.seek 0.82ml
@18:44:49.17 delay 3 seconds
@18:44:52.20 ATV.dial PCR
@18:44:52.63 AS.reconfigure ASconfig
@18:44:52.68 AS.seek 0ml
@18:45:40.08 PCR.program :bleachProfile
@18:45:40.15 PCR program should complete in < 2:45
@18:45:40.16 PCR.run 10:00
@18:48:35.95 PCR.off
@18:48:35.99 AFV.select :carrierSolution
@18:48:36.10 delay 2 seconds
@18:48:38.12 AS.seek 1ml
@18:49:35.87 delay 1 second
@18:49:36.89 AFV.select :holdingCoil
@18:49:37.00 delay 1 second
@18:49:38.02 AS.reconfigure ASfast
@18:49:38.08 AS.seek 0ml
@18:50:05.42 delay 3 seconds
@18:50:08.44 AFV.select :carrierSolution
@18:50:08.55 delay 2 seconds
@18:50:10.57 AS.reconfigure ASconfig
@18:50:10.63 AS.seek 1ml
@18:51:08.42 delay 1 second
@18:51:09.44 AFV.select :holdingCoil
@18:51:09.55 delay 1 second
@18:51:10.57 AS.reconfigure ASfast
@18:51:10.63 AS.seek 0ml
@18:51:37.98 delay 3 seconds
@18:51:41.00 AFV.select :carrierSolution
@18:51:41.11 delay 2 seconds
@18:51:43.13 AS.reconfigure ASconfig
@18:51:43.18 AS.seek 1ml
@18:52:40.94 delay 1 second
@18:52:41.96 AFV.select :holdingCoil
@18:52:42.07 delay 1 second
@18:52:43.09 AS.reconfigure ASfast
@18:52:43.15 AS.seek 0ml
@18:53:10.48 delay 3 seconds
@18:53:13.50 AFV.select :carrierSolution
@18:53:13.61 delay 2 seconds
@18:53:15.63 AS.reconfigure ASconfig
@18:53:15.69 AS.seek 1ml
@18:54:13.47 delay 1 second
@18:54:14.49 AFV.select :holdingCoil
@18:54:14.60 delay 1 second
@18:54:16.03 AS.reconfigure ASfast
@18:54:16.09 AS.seek 0ml
@18:54:43.44 delay 3 seconds
@18:54:46.46 AFV.select :carrierSolution
@18:54:46.57 delay 2 seconds
@18:54:48.59 AS.reconfigure ASconfig
@18:54:48.65 AS.seek 1ml
@18:55:46.39 delay 1 second
@18:55:47.41 AFV.select :holdingCoil
@18:55:47.52 delay 1 second
@18:55:48.54 AS.reconfigure ASnorm
@18:55:48.60 AS.seek 0ml
@18:56:39.33 Setting up PCR for AALPP3 -- writing /var/log/esp/AALPP321apr0818h.pcr
@18:56:39.35 Building PCR Slug from ASV with MM2 mix, PP3 primer
@18:56:39.37 Priming with carrier
@18:56:39.38 ATV.dial ARV
@18:56:40.03 ARV.dial :carrier
@18:56:40.39 AS.seek 0.5ml
@18:57:05.95 AFV.select :carrierSolution
@18:57:06.08 delay 2 seconds
@18:57:08.10 AS.reconfigure ASconfig
@18:57:08.17 AS.seek 1ml
@18:57:37.20 delay 1 second
@18:57:38.22 AFV.select :holdingCoil
@18:57:38.33 delay 1 second
@18:57:39.35 ATV.dial :waste
@18:57:41.12 AS.reconfigure ASnorm
@18:57:41.18 AS.seek 0ml
@18:58:31.86 AFV.select :carrierSolution
@18:58:31.99 delay 2 seconds
@18:58:34.01 AS.reconfigure ASconfig
@18:58:34.07 AS.seek 0.5ml
@18:59:03.09 delay 1 second
@18:59:04.11 AFV.select :holdingCoil
@18:59:04.22 Building Slug
@18:59:04.23 ATV.dial :ARV
@18:59:05.97 delay 2 seconds
@18:59:08.00 ARV.dial :air1
@18:59:08.40 delay 2 seconds
@18:59:10.47 AS.reconfigure ASslow2
@18:59:10.53 AS.seek 0.506ml
@18:59:16.91 delay 2 seconds
@18:59:18.94 ARV.dial :MM2
@18:59:20.53 delay 2 seconds
@18:59:22.56 Adding MM2
@18:59:22.63 AS.seek 0.524ml
@18:59:41.06 delay 25 seconds
@19:00:06.09 ARV.dial :PP3
@19:00:06.49 Adding PP3
@19:00:06.50 delay 2 seconds
@19:00:08.57 AS.seek 0.53ml
@19:00:14.89 delay 10 seconds
@19:00:24.93 ARV.dial :air2
@19:00:25.35 adding ARV air
@19:00:25.36 delay 2 seconds
@19:00:27.43 AS.seek 0.536ml
@19:00:34.14 delay 5 seconds
@19:00:39.17 ARV.dial :carrier
@19:00:40.53 delay 7 seconds
@19:00:47.61 AS.seek 0.594ml
@19:01:46.31 delay 5 seconds
@19:01:51.34 ATV.dial :ASV
@19:01:51.76 delay 2 seconds
@19:01:53.83 AS.seek 0.6ml
@19:02:00.01 delay 4 seconds
@19:02:04.04 ATV.dial ARV
@19:02:04.62 delay 2 seconds
@19:02:06.65 ARV.dial :carrier
@19:02:06.66 delay 2 seconds
@19:02:08.73 AS.seek 0.65ml
@19:02:59.19 delay 4 seconds
@19:03:03.22 ATV.dial PCR
@19:03:03.86 ASV.dial :waste1
@19:03:03.88 ARV.dial :air1
@19:03:04.32 Positioning Slug by 0.324ml
@19:03:04.40 AS.seek 0.326ml
@19:08:30.64 delay 5 seconds
@19:08:35.67 ATV.dial ARV
@19:08:36.48 ASV.dial :PCRlock
@19:08:36.89 delay 5 seconds
@19:08:41.92 Running AALPP3 Profile58CIQ2 thermocycling
@19:08:41.93 <PCR> PCR.program Profile58CIQ2
@19:08:42.05 Profile58CIQ2 should complete in < 1:36:15
@19:08:42.06 PCR.run 2:05:18.75
@20:29:33.15 PCR.off
@20:29:33.72 <MFBPCR> Running AALPP3 MeltProfile58 thermocycling
@20:29:33.74 <PCR> PCR.program MeltProfile58
@20:29:33.84 MeltProfile58 should complete in < 12:33
@20:29:33.85 PCR.run 20:41.25
@20:37:21.59 PCR.off
@20:37:22.58 <MFBPCR> PCR for AALPP3 completed -- closed /var/log/esp/AALPP321apr0818h.pcr
@20:37:22.65 Cleaning PCR
@20:37:22.66 AFV.select :carrierSolution
@20:37:22.78 delay 2 seconds
@20:37:24.80 AS.reconfigure ASconfig
@20:37:24.85 AS.seek 0.5ml
@20:37:35.14 delay 1 second
@20:37:36.54 AFV.select :holdingCoil
@20:37:36.65 ASV.dial :waste1
@20:37:37.05 ATV.dial :air3
@20:37:38.40 AS.reconfigure ASslow1
@20:37:38.46 AS.seek 0.51ml
@20:37:40.76 delay 2 seconds
@20:37:42.79 ATV.dial :bleach
@20:37:43.45 AS.seek 0.81ml
@20:38:44.01 delay 3 seconds
@20:38:47.04 ATV.dial :air3
@20:38:47.62 delay 3 seconds
@20:38:50.69 AS.seek 0.835ml
@20:38:56.14 delay 3 seconds
@20:38:59.17 ATV.dial :bleach
@20:38:59.81 AS.seek 0.82ml
@20:39:03.11 delay 3 seconds
@20:39:06.14 ARV.dial :waste
@20:39:06.78 delay 3 seconds
@20:39:09.81 ATV.dial :ARV
@20:39:10.73 AS.reconfigure ASconfig
@20:39:10.79 AS.seek 0.47ml
@20:39:31.20 AFV.select :carrierSolution
@20:39:31.32 delay 2 seconds
@20:39:33.34 AS.seek 1ml
@20:40:04.08 delay 1 second
@20:40:05.10 AFV.select :holdingCoil
@20:40:05.21 delay 1 second
@20:40:06.23 AS.reconfigure ASfast
@20:40:06.29 AS.seek 0ml
@20:40:33.62 AFV.select :carrierSolution
@20:40:33.74 delay 2 seconds
@20:40:35.76 AS.reconfigure ASconfig
@20:40:35.81 AS.seek 1ml
@20:41:33.56 delay 1 second
@20:41:34.58 AFV.select :holdingCoil
@20:41:34.69 delay 1 second
@20:41:35.71 AS.reconfigure ASfast
@20:41:35.77 AS.seek 0ml
@20:42:03.09 AFV.select :carrierSolution
@20:42:03.21 delay 2 seconds
@20:42:05.23 AS.reconfigure ASconfig
@20:42:05.28 AS.seek 1ml
@20:43:03.06 delay 1 second
@20:43:04.08 AFV.select :holdingCoil
@20:43:04.19 delay 1 second
@20:43:05.21 AS.reconfigure ASfast
@20:43:05.27 AS.seek 0ml
@20:43:32.62 ARV.dial between carrier and air1
@20:43:33.17 ATV.dial :air3
@20:43:34.51 AS.reconfigure ASslow1
@20:43:34.57 AS.seek 0.01ml
@20:43:36.95 delay 2 seconds
@20:43:38.98 ATV.dial :bleach
@20:43:39.64 AS.seek 0.81ml
@20:46:20.92 delay 3 seconds
@20:46:23.95 ATV.dial :air3
@20:46:24.53 delay 3 seconds
@20:46:27.60 AS.seek 0.835ml
@20:46:32.96 delay 3 seconds
@20:46:35.99 ATV.dial :bleach
@20:46:36.62 AS.seek 0.82ml
@20:46:39.89 delay 3 seconds
@20:46:42.92 ATV.dial PCR
@20:46:43.34 AS.reconfigure ASconfig
@20:46:43.39 AS.seek 0ml
@20:47:30.80 PCR.program :bleachProfile
@20:47:30.87 PCR program should complete in < 2:45
@20:47:30.88 PCR.run 10:00
@20:50:27.10 PCR.off
@20:50:27.14 AFV.select :carrierSolution
@20:50:27.25 delay 2 seconds
@20:50:29.27 AS.seek 1ml
@20:51:27.03 delay 1 second
@20:51:28.05 AFV.select :holdingCoil
@20:51:28.16 delay 1 second
@20:51:29.18 AS.reconfigure ASfast
@20:51:29.24 AS.seek 0ml
@20:51:56.58 delay 3 seconds
@20:51:59.60 AFV.select :carrierSolution
@20:51:59.71 delay 2 seconds
@20:52:01.73 AS.reconfigure ASconfig
@20:52:01.80 AS.seek 1ml
@20:52:59.55 delay 1 second
@20:53:00.57 AFV.select :holdingCoil
@20:53:00.68 delay 1 second
@20:53:01.70 AS.reconfigure ASfast
@20:53:01.76 AS.seek 0ml
@20:53:29.11 delay 3 seconds
@20:53:32.13 AFV.select :carrierSolution
@20:53:32.24 delay 2 seconds
@20:53:34.26 AS.reconfigure ASconfig
@20:53:34.33 AS.seek 1ml
@20:54:32.09 delay 1 second
@20:54:33.11 AFV.select :holdingCoil
@20:54:33.22 delay 1 second
@20:54:34.24 AS.reconfigure ASfast
@20:54:34.30 AS.seek 0ml
@20:55:01.67 delay 3 seconds
@20:55:04.69 AFV.select :carrierSolution
@20:55:04.80 delay 2 seconds
@20:55:06.82 AS.reconfigure ASconfig
@20:55:06.89 AS.seek 1ml
@20:56:04.67 delay 1 second
@20:56:05.69 AFV.select :holdingCoil
@20:56:05.80 delay 1 second
@20:56:06.82 AS.reconfigure ASfast
@20:56:06.88 AS.seek 0ml
@20:56:34.23 delay 3 seconds
@20:56:37.25 AFV.select :carrierSolution
@20:56:37.36 delay 2 seconds
@20:56:39.38 AS.reconfigure ASconfig
@20:56:39.45 AS.seek 1ml
@20:57:37.20 delay 1 second
@20:57:38.22 AFV.select :holdingCoil
@20:57:38.33 delay 1 second
@20:57:39.35 AS.reconfigure ASnorm
@20:57:39.40 AS.seek 0ml
@20:58:30.12 Setting up PCR for ACOPP5 -- writing /var/log/esp/ACOPP521apr0820h.pcr
@20:58:30.13 Building PCR Slug from ASV with MM3 mix, PP5 primer
@20:58:30.16 Priming with carrier
@20:58:30.18 ATV.dial ARV
@20:58:30.83 ARV.dial :carrier
@20:58:31.18 AS.seek 0.5ml
@20:58:56.70 AFV.select :carrierSolution
@20:58:56.82 delay 2 seconds
@20:58:58.84 AS.reconfigure ASconfig
@20:58:58.90 AS.seek 1ml
@20:59:27.91 delay 1 second
@20:59:28.93 AFV.select :holdingCoil
@20:59:29.04 delay 1 second
@20:59:30.06 ATV.dial :waste
@20:59:31.84 AS.reconfigure ASnorm
@20:59:31.90 AS.seek 0ml
@21:00:22.62 AFV.select :carrierSolution
@21:00:22.74 delay 2 seconds
@21:00:24.76 AS.reconfigure ASconfig
@21:00:24.82 AS.seek 0.5ml
@21:00:53.86 delay 1 second
@21:00:54.88 AFV.select :holdingCoil
@21:00:54.99 Building Slug
@21:00:55.00 ATV.dial :ARV
@21:00:56.75 delay 2 seconds
@21:00:58.78 ARV.dial :air1
@21:00:59.17 delay 2 seconds
@21:01:01.24 AS.reconfigure ASslow2
@21:01:01.30 AS.seek 0.506ml
@21:01:07.81 delay 2 seconds
@21:01:09.84 ARV.dial :MM3
@21:01:10.94 delay 2 seconds
@21:01:12.97 Adding MM3
@21:01:13.05 AS.seek 0.524ml
@21:01:31.62 delay 25 seconds
@21:01:56.65 ARV.dial :PP5
@21:01:57.07 Adding PP5
@21:01:57.08 delay 2 seconds
@21:01:59.15 AS.seek 0.53ml
@21:02:05.67 delay 10 seconds
@21:02:15.71 ARV.dial :air3
@21:02:16.13 adding ARV air
@21:02:16.14 delay 2 seconds
@21:02:18.21 AS.seek 0.536ml
@21:02:24.47 delay 5 seconds
@21:02:29.50 ARV.dial :carrier
@21:02:30.84 delay 7 seconds
@21:02:37.92 AS.seek 0.594ml
@21:03:36.37 delay 5 seconds
@21:03:41.40 ATV.dial :ASV
@21:03:41.81 delay 2 seconds
@21:03:43.88 AS.seek 0.6ml
@21:03:50.31 delay 4 seconds
@21:03:54.34 ATV.dial ARV
@21:03:54.91 delay 2 seconds
@21:03:56.94 ARV.dial :carrier
@21:03:56.95 delay 2 seconds
@21:03:59.02 AS.seek 0.65ml
@21:04:49.94 delay 4 seconds
@21:04:53.97 ATV.dial PCR
@21:04:54.61 ASV.dial :waste1
@21:04:54.63 ARV.dial :air1
@21:04:55.06 Positioning Slug by 0.324ml
@21:04:55.57 AS.seek 0.326ml
@21:10:21.56 delay 5 seconds
@21:10:26.59 ATV.dial ARV
@21:10:27.41 ASV.dial :PCRlock
@21:10:27.81 delay 5 seconds
@21:10:32.84 Running ACOPP5 ProfileIPc thermocycling
@21:10:32.85 <PCR> PCR.program ProfileIPc
@21:10:32.98 ProfileIPc should complete in < 1:26:35
@21:10:32.99 PCR.run 1:53:13.75
@22:19:14.89 PCR.off
@22:19:15.84 <MFBPCR> PCR for ACOPP5 completed -- closed /var/log/esp/ACOPP521apr0820h.pcr
@22:19:15.92 Cleaning PCR
@22:19:15.93 AFV.select :carrierSolution
@22:19:16.06 delay 2 seconds
@22:19:18.08 AS.reconfigure ASconfig
@22:19:18.13 AS.seek 0.5ml
@22:19:28.41 delay 1 second
@22:19:29.43 AFV.select :holdingCoil
@22:19:29.54 ASV.dial :waste1
@22:19:29.94 ATV.dial :air3
@22:19:31.29 AS.reconfigure ASslow1
@22:19:31.35 AS.seek 0.51ml
@22:19:33.64 delay 2 seconds
@22:19:35.67 ATV.dial :bleach
@22:19:36.33 AS.seek 0.81ml
@22:20:37.02 delay 3 seconds
@22:20:40.05 ATV.dial :air3
@22:20:40.63 delay 3 seconds
@22:20:43.70 AS.seek 0.835ml
@22:20:49.03 delay 3 seconds
@22:20:52.06 ATV.dial :bleach
@22:20:52.70 AS.seek 0.82ml
@22:20:56.03 delay 3 seconds
@22:20:59.06 ARV.dial :waste
@22:20:59.70 delay 3 seconds
@22:21:02.73 ATV.dial :ARV
@22:21:03.65 AS.reconfigure ASconfig
@22:21:03.71 AS.seek 0.47ml
@22:21:24.11 AFV.select :carrierSolution
@22:21:24.23 delay 2 seconds
@22:21:26.25 AS.seek 1ml
@22:21:56.98 delay 1 second
@22:21:58.00 AFV.select :holdingCoil
@22:21:58.11 delay 1 second
@22:21:59.13 AS.reconfigure ASfast
@22:21:59.19 AS.seek 0ml
@22:22:26.50 AFV.select :carrierSolution
@22:22:26.62 delay 2 seconds
@22:22:28.64 AS.reconfigure ASconfig
@22:22:28.69 AS.seek 1ml
@22:23:26.44 delay 1 second
@22:23:27.46 AFV.select :holdingCoil
@22:23:27.57 delay 1 second
@22:23:28.59 AS.reconfigure ASfast
@22:23:28.65 AS.seek 0ml
@22:23:56.00 AFV.select :carrierSolution
@22:23:56.12 delay 2 seconds
@22:23:58.14 AS.reconfigure ASconfig
@22:23:58.19 AS.seek 1ml
@22:24:55.94 delay 1 second
@22:24:56.96 AFV.select :holdingCoil
@22:24:57.07 delay 1 second
@22:24:58.09 AS.reconfigure ASfast
@22:24:58.15 AS.seek 0ml
@22:25:25.48 ARV.dial between carrier and air1
@22:25:26.01 ATV.dial :air3
@22:25:27.36 AS.reconfigure ASslow1
@22:25:27.42 AS.seek 0.01ml
@22:25:29.70 delay 2 seconds
@22:25:31.73 ATV.dial :bleach
@22:25:32.39 AS.seek 0.81ml
@22:28:13.55 delay 3 seconds
@22:28:16.58 ATV.dial :air3
@22:28:17.16 delay 3 seconds
@22:28:20.23 AS.seek 0.835ml
@22:28:25.54 delay 3 seconds
@22:28:28.57 ATV.dial :bleach
@22:28:29.20 AS.seek 0.82ml
@22:28:32.52 delay 3 seconds
@22:28:35.55 ATV.dial PCR
@22:28:35.96 AS.reconfigure ASconfig
@22:28:36.01 AS.seek 0ml
@22:29:23.41 PCR.program :bleachProfile
@22:29:23.49 PCR program should complete in < 2:45
@22:29:23.50 PCR.run 10:00
@22:32:19.71 PCR.off
@22:32:19.75 AFV.select :carrierSolution
@22:32:19.86 delay 2 seconds
@22:32:21.88 AS.seek 1ml
@22:33:19.64 delay 1 second
@22:33:20.66 AFV.select :holdingCoil
@22:33:20.77 delay 1 second
@22:33:21.79 AS.reconfigure ASfast
@22:33:21.85 AS.seek 0ml
@22:33:49.17 delay 3 seconds
@22:33:52.19 AFV.select :carrierSolution
@22:33:52.30 delay 2 seconds
@22:33:54.32 AS.reconfigure ASconfig
@22:33:54.38 AS.seek 1ml
@22:34:52.14 delay 1 second
@22:34:53.16 AFV.select :holdingCoil
@22:34:53.27 delay 1 second
@22:34:54.29 AS.reconfigure ASfast
@22:34:54.35 AS.seek 0ml
@22:35:21.67 delay 3 seconds
@22:35:24.69 AFV.select :carrierSolution
@22:35:24.80 delay 2 seconds
@22:35:26.82 AS.reconfigure ASconfig
@22:35:26.89 AS.seek 1ml
@22:36:24.66 delay 1 second
@22:36:25.68 AFV.select :holdingCoil
@22:36:25.79 delay 1 second
@22:36:26.81 AS.reconfigure ASfast
@22:36:26.87 AS.seek 0ml
@22:36:54.20 delay 3 seconds
@22:36:57.22 AFV.select :carrierSolution
@22:36:57.33 delay 2 seconds
@22:36:59.35 AS.reconfigure ASconfig
@22:36:59.41 AS.seek 1ml
@22:37:57.16 delay 1 second
@22:37:58.18 AFV.select :holdingCoil
@22:37:58.29 delay 1 second
@22:37:59.31 AS.reconfigure ASfast
@22:37:59.37 AS.seek 0ml
@22:38:26.70 delay 3 seconds
@22:38:29.72 AFV.select :carrierSolution
@22:38:29.83 delay 2 seconds
@22:38:31.85 AS.reconfigure ASconfig
@22:38:31.91 AS.seek 1ml
@22:39:29.67 delay 1 second
@22:39:30.69 AFV.select :holdingCoil
@22:39:30.80 delay 1 second
@22:39:31.82 AS.reconfigure ASnorm
@22:39:31.88 AS.seek 0ml
@22:40:22.64 Setting up PCR for AIPc -- writing /var/log/esp/AIPc21apr0822h.pcr
@22:40:22.65 Building PCR Slug from ASV with MM3 mix, PP6 primer
@22:40:22.68 Priming with carrier
@22:40:22.69 ATV.dial ARV
@22:40:23.44 ARV.dial :carrier
@22:40:23.80 AS.seek 0.5ml
@22:40:49.33 AFV.select :carrierSolution
@22:40:49.46 delay 2 seconds
@22:40:51.48 AS.reconfigure ASconfig
@22:40:51.54 AS.seek 1ml
@22:41:20.56 delay 1 second
@22:41:21.58 AFV.select :holdingCoil
@22:41:21.69 delay 1 second
@22:41:22.71 ATV.dial :waste
@22:41:24.48 AS.reconfigure ASnorm
@22:41:24.54 AS.seek 0ml
@22:42:15.23 AFV.select :carrierSolution
@22:42:15.36 delay 2 seconds
@22:42:17.38 AS.reconfigure ASconfig
@22:42:17.44 AS.seek 0.5ml
@22:42:46.47 delay 1 second
@22:42:47.49 AFV.select :holdingCoil
@22:42:47.60 Building Slug
@22:42:47.61 ATV.dial :ARV
@22:42:49.34 delay 2 seconds
@22:42:51.37 ARV.dial :air1
@22:42:51.79 delay 2 seconds
@22:42:53.86 AS.reconfigure ASslow2
@22:42:53.92 AS.seek 0.506ml
@22:43:00.44 delay 2 seconds
@22:43:02.47 ARV.dial :MM3
@22:43:03.56 delay 2 seconds
@22:43:05.59 Adding MM3
@22:43:05.67 AS.seek 0.524ml
@22:43:24.67 delay 25 seconds
@22:43:49.70 ARV.dial :PP6
@22:43:50.09 Adding PP6
@22:43:50.11 delay 2 seconds
@22:43:52.18 AS.seek 0.53ml
@22:43:58.83 delay 10 seconds
@22:44:08.87 ARV.dial :air1
@22:44:09.75 adding ARV air
@22:44:09.76 delay 2 seconds
@22:44:11.83 AS.seek 0.536ml
@22:44:17.99 delay 5 seconds
@22:44:23.02 ARV.dial :carrier
@22:44:23.42 delay 7 seconds
@22:44:30.50 AS.seek 0.594ml
@22:45:29.17 delay 5 seconds
@22:45:34.20 ATV.dial :ASV
@22:45:34.63 delay 2 seconds
@22:45:36.70 AS.seek 0.6ml
@22:45:42.97 delay 4 seconds
@22:45:47.00 ATV.dial ARV
@22:45:47.56 delay 2 seconds
@22:45:50.00 ARV.dial :carrier
@22:45:50.03 delay 2 seconds
@22:45:52.11 AS.seek 0.65ml
@22:46:42.63 delay 4 seconds
@22:46:46.66 ATV.dial PCR
@22:46:47.31 ASV.dial :waste1
@22:46:47.33 ARV.dial :air1
@22:46:47.76 Positioning Slug by 0.324ml
@22:46:47.85 AS.seek 0.326ml
@22:52:14.22 delay 5 seconds
@22:52:19.25 ATV.dial ARV
@22:52:20.06 ASV.dial :PCRlock
@22:52:20.47 delay 5 seconds
@22:52:25.50 Running AIPc ProfileIPc thermocycling
@22:52:25.51 <PCR> PCR.program ProfileIPc
@22:52:25.63 ProfileIPc should complete in < 1:26:35
@22:52:25.64 PCR.run 1:53:13.75
@00:00:59.73CEST09-Apr-21 PCR.off
@00:01:00.30 <MFBPCR> PCR for AIPc completed -- closed /var/log/esp/AIPc21apr0822h.pcr
@00:01:00.32 Full MFB Decontamination
@00:01:00.34 Decon extraction column and decon extSrc
@00:01:00.37 SPE.hold 2:46:40,55C
@00:01:00.41 ASV.dial :SPEdelivery
@00:01:01.10 delay 20 seconds
@00:01:21.13 AFV.select :carrierSolution
@00:01:21.24 delay 2 seconds
@00:01:23.26 AS.reconfigure ASconfig
@00:01:23.32 AS.seek 0.5ml
@00:01:33.63 delay 1 second
@00:01:34.65 AFV.select :holdingCoil
@00:01:34.76 ATV.dial :air1
@00:01:37.01 AS.reconfigure ASslow1
@00:01:37.07 AS.seek 0.52ml
@00:01:41.38 ATV.dial :ASV
@00:01:43.14 ASV.dial :SPErecovery
@00:01:43.84 AS.seek 0.62ml
@00:02:04.22 ATV.dial :waste
@00:02:06.42 AS.reconfigure ASnorm
@00:02:06.48 AS.seek 0ml
@00:02:38.06 deconEluateRecoveryLine extSrc
@00:02:38.07 ATV.dial :waste
@00:02:38.11 AS.seek 0ml
@00:02:38.38 ATV.dial :air3
@00:02:39.16 AS.reconfigure ASslow1
@00:02:39.22 AS.seek 0.01ml
@00:02:41.52 delay 2 seconds
@00:02:43.55 ATV.dial :bleach
@00:02:44.06 AS.seek 0.31ml
@00:03:44.64 delay 3 seconds
@00:03:47.67 ATV.dial :air3
@00:03:48.30 delay 3 seconds
@00:03:51.38 AS.seek 0.335ml
@00:03:56.61 delay 3 seconds
@00:03:59.64 ATV.dial :bleach
@00:04:00.31 AS.seek 0.32ml
@00:04:03.75 delay 3 seconds
@00:04:06.78 ATV.dial :extSrc
@00:04:07.47 AS.seek 0ml
@00:05:12.05 AFV.select :carrierSolution
@00:05:12.17 delay 2 seconds
@00:05:14.19 AS.reconfigure ASconfig
@00:05:14.25 AS.seek 0.5ml
@00:05:43.28 delay 1 second
@00:05:44.30 AFV.select :holdingCoil
@00:05:44.41 ATV.dial :extSrc
@00:05:44.43 AS.reconfigure ASslow1
@00:05:44.50 AS.seek 0ml
@00:07:25.33 AFV.select :carrierSolution
@00:07:25.45 delay 2 seconds
@00:07:27.47 AS.reconfigure ASconfig
@00:07:27.53 AS.seek 0.5ml
@00:07:56.56 delay 1 second
@00:07:57.58 AFV.select :holdingCoil
@00:07:57.69 ATV.dial :extSrc
@00:07:57.71 AS.reconfigure ASslow1
@00:07:57.78 AS.seek 0ml
@00:09:38.70 AFV.select :carrierSolution
@00:09:38.82 delay 2 seconds
@00:09:40.84 AS.reconfigure ASconfig
@00:09:40.90 AS.seek 0.5ml
@00:10:09.92 delay 1 second
@00:10:10.94 AFV.select :holdingCoil
@00:10:11.05 ATV.dial :extSrc
@00:10:11.07 AS.reconfigure ASslow1
@00:10:11.14 AS.seek 0ml
@00:11:51.97 AFV.select :carrierSolution
@00:11:52.09 delay 2 seconds
@00:11:54.11 AS.reconfigure ASconfig
@00:11:54.17 AS.seek 0.5ml
@00:12:23.20 delay 1 second
@00:12:24.22 AFV.select :holdingCoil
@00:12:24.33 ATV.dial :extSrc
@00:12:24.35 AS.reconfigure ASslow1
@00:12:24.42 AS.seek 0ml
@00:14:05.24 ATV.dial :air2
@00:14:06.64 AS.reconfigure ASslow0
@00:14:06.70 AS.seek 0.5ml
@00:14:57.30 ATV.dial :extSrc
@00:14:58.87 AS.seek 0.02ml
@00:15:47.49 delay 1 second
@00:15:48.52 ATV.dial :waste
@00:15:49.87 AS.reconfigure ASconfig
@00:15:49.93 AS.seek 0ml
@00:15:51.38 AFV.select :holdingCoil
@00:15:51.39 ATV.dial :waste
@00:15:51.42 AS.seek 0ml
@00:15:51.51 AFV.select :carrierSolution
@00:15:51.62 delay 2 seconds
@00:15:53.64 AS.seek 0.5ml
@00:16:22.66 delay 1 second
@00:16:23.68 AFV.select :holdingCoil
@00:16:23.79 ATV.dial :air3
@00:16:24.67 AS.reconfigure ASslow1
@00:16:24.73 AS.seek 0.52ml
@00:16:29.05 ATV.dial :air3
@00:16:29.14 AS.seek 0.53ml
@00:16:31.49 delay 2 seconds
@00:16:33.95 ATV.dial :bleach
@00:16:34.43 AS.seek 0.71ml
@00:17:10.91 delay 3 seconds
@00:17:13.94 ATV.dial :air3
@00:17:14.52 delay 3 seconds
@00:17:17.60 AS.seek 0.735ml
@00:17:22.92 delay 3 seconds
@00:17:25.95 ATV.dial :bleach
@00:17:26.59 AS.seek 0.72ml
@00:17:29.94 delay 3 seconds
@00:17:32.97 ATV.dial ASV
@00:17:34.06 ASV.dial :SPErecovery
@00:17:34.15 AS.seek 0.365ml
@00:18:45.83 ATV.dial :waste
@00:18:47.84 AS.reconfigure ASnorm
@00:18:47.90 AS.seek 0ml
@00:19:06.63 ATV.dial :air3
@00:19:07.52 AS.reconfigure ASslow1
@00:19:07.58 AS.seek 0.01ml
@00:19:10.06 delay 2 seconds
@00:19:12.09 ATV.dial :bleach
@00:19:12.56 AS.seek 0.58ml
@00:21:07.50 delay 3 seconds
@00:21:10.53 ATV.dial :air3
@00:21:11.11 delay 3 seconds
@00:21:14.19 AS.seek 0.605ml
@00:21:19.51 delay 3 seconds
@00:21:22.54 ATV.dial :bleach
@00:21:23.17 AS.seek 0.59ml
@00:21:26.47 delay 3 seconds
@00:21:29.50 ATV.dial :ASV
@00:21:30.60 ASV.dial :wasteR
@00:21:31.01 AS.seek 0ml
@00:23:30.06 delay 1:00
@00:24:30.10 ATV.dial ASV
@00:24:30.11 AFV.select :carrierSolution
@00:24:30.23 delay 2 seconds
@00:24:32.25 AS.reconfigure ASconfig
@00:24:32.30 AS.seek 1ml
@00:25:30.11 delay 1 second
@00:25:31.13 AFV.select :holdingCoil
@00:25:31.24 delay 1 second
@00:25:32.26 ASV.dial :wasteR
@00:25:32.27 AS.reconfigure ASslow1
@00:25:32.35 AS.seek 0ml
@00:28:53.74 delay 10 seconds
@00:29:03.78 ATV.dial :air3
@00:29:05.37 AS.seek 0.01ml
@00:29:07.69 delay 2 seconds
@00:29:09.72 ATV.dial :bleach
@00:29:10.37 AS.seek 0.58ml
@00:31:05.31 delay 3 seconds
@00:31:08.34 ATV.dial :air3
@00:31:08.92 delay 3 seconds
@00:31:11.99 AS.seek 0.605ml
@00:31:17.33 delay 3 seconds
@00:31:20.36 ATV.dial :bleach
@00:31:21.02 AS.seek 0.59ml
@00:31:24.33 delay 3 seconds
@00:31:27.36 ATV.dial ASV
@00:31:28.46 ASV.dial :SPEdelivery
@00:31:28.86 AS.seek 0ml
@00:33:27.78 delay 1:00
@00:34:27.81 AFV.select :carrierSolution
@00:34:27.92 delay 2 seconds
@00:34:29.94 AS.reconfigure ASconfig
@00:34:30.00 AS.seek 1ml
@00:35:27.75 delay 1 second
@00:35:28.77 AFV.select :holdingCoil
@00:35:28.88 delay 1 second
@00:35:29.90 ATV.dial ASV
@00:35:29.91 ASV.dial :SPEdelivery
@00:35:29.94 AS.reconfigure ASslow1
@00:35:30.01 AS.seek 0ml
@00:38:51.41 delay 10 seconds
@00:39:01.45 ATV.dial :air2
@00:39:02.34 AS.reconfigure ASslow0
@00:39:02.40 AS.seek 0.8ml
@00:40:23.11 delay 3 seconds
@00:40:26.14 ATV.dial :ASV
@00:40:27.19 ASV.dial :SPEdelivery
@00:40:27.21 AS.reconfigure ASslow1
@00:40:27.28 AS.seek 0.03ml
@00:43:02.38 delay 20 seconds
@00:43:22.42 ATV.dial :waste,via: :air3
@00:43:24.26 AS.reconfigure ASconfig
@00:43:24.31 AS.seek 0ml
@00:43:26.33 SPE.coast
@00:43:26.41 Removing Bleach from Mixing Coil
@00:43:26.43 AFV.select :carrierSolution
@00:43:26.54 delay 2 seconds
@00:43:28.56 AS.seek 0.5ml
@00:43:57.58 delay 1 second
@00:43:58.60 AFV.select :holdingCoil
@00:43:58.71 ATV.dial :ASV
@00:44:00.52 ASV.dial :SPErecovery
@00:44:01.17 AS.reconfigure ASslow1
@00:44:01.23 AS.seek 0.73ml
@00:44:47.80 ASV.dial :wasteD
@00:44:48.42 AS.reconfigure ASnorm
@00:44:48.47 AS.seek 0ml
@00:45:25.56 AFV.select :carrierSolution
@00:45:25.68 delay 2 seconds
@00:45:27.70 AS.reconfigure ASconfig
@00:45:27.76 AS.seek 0.5ml
@00:45:56.80 delay 1 second
@00:45:57.82 AFV.select :holdingCoil
@00:45:57.93 ATV.dial ASV
@00:45:57.94 ASV.dial :SPErecovery
@00:45:59.04 AS.reconfigure ASslow0
@00:45:59.10 AS.seek 0.3ml
@00:46:19.56 ATV.dial :air2
@00:46:20.66 AS.reconfigure ASslow1
@00:46:20.72 AS.seek 0.32ml
@00:46:25.05 ATV.dial ASV
@00:46:26.16 AS.reconfigure ASslow0
@00:46:26.22 AS.seek 0.57ml
@00:46:51.71 ASV.dial :wasteD
@00:46:52.32 AS.reconfigure ASnorm
@00:46:52.38 AS.seek 0ml
@00:47:21.42 AFV.select :carrierSolution
@00:47:21.54 delay 2 seconds
@00:47:23.56 AS.reconfigure ASconfig
@00:47:23.62 AS.seek 0.5ml
@00:47:52.64 delay 1 second
@00:47:53.66 AFV.select :holdingCoil
@00:47:53.77 ATV.dial ASV
@00:47:53.78 ASV.dial :SPErecovery
@00:47:54.44 AS.reconfigure ASslow0
@00:47:54.50 AS.seek 0.3ml
@00:48:14.96 ATV.dial :air2
@00:48:16.06 AS.reconfigure ASslow1
@00:48:16.12 AS.seek 0.32ml
@00:48:20.46 ATV.dial ASV
@00:48:21.56 AS.reconfigure ASslow0
@00:48:21.62 AS.seek 0.57ml
@00:48:47.07 ASV.dial :wasteD
@00:48:47.68 AS.reconfigure ASnorm
@00:48:47.74 AS.seek 0ml
@00:49:16.78 ATV.dial :air2
@00:49:17.82 AS.reconfigure ASslow0
@00:49:17.88 AS.seek 0.3ml
@00:49:48.39 ATV.dial :ASV
@00:49:49.42 ASV.dial :wasteD
@00:49:49.44 AS.reconfigure ASconfig
@00:49:49.51 AS.seek 0.05ml
@00:50:04.17 ATV.dial :waste
@00:50:06.17 AS.seek 0ml
@00:50:09.34 ASV.dial :SPEdelivery
@00:50:10.44 Cleaning PCR
@00:50:10.45 AFV.select :carrierSolution
@00:50:10.57 delay 2 seconds
@00:50:12.59 AS.seek 0.5ml
@00:50:41.60 delay 1 second
@00:50:42.62 AFV.select :holdingCoil
@00:50:42.73 ASV.dial :waste1
@00:50:43.11 ATV.dial :air3
@00:50:43.84 AS.reconfigure ASslow1
@00:50:43.90 AS.seek 0.51ml
@00:50:46.30 delay 2 seconds
@00:50:48.33 ATV.dial :bleach
@00:50:48.80 AS.seek 0.81ml
@00:51:49.50 delay 3 seconds
@00:51:52.53 ATV.dial :air3
@00:51:53.11 delay 3 seconds
@00:51:56.18 AS.seek 0.835ml
@00:52:01.48 delay 3 seconds
@00:52:04.51 ATV.dial :bleach
@00:52:05.16 AS.seek 0.82ml
@00:52:08.47 delay 3 seconds
@00:52:11.50 ARV.dial :waste
@00:52:12.13 delay 3 seconds
@00:52:15.16 ATV.dial :ARV
@00:52:16.08 AS.reconfigure ASconfig
@00:52:16.14 AS.seek 0.47ml
@00:52:36.55 AFV.select :carrierSolution
@00:52:36.67 delay 2 seconds
@00:52:38.69 AS.seek 1ml
@00:53:09.47 delay 1 second
@00:53:10.49 AFV.select :holdingCoil
@00:53:10.60 delay 1 second
@00:53:11.62 AS.reconfigure ASfast
@00:53:11.68 AS.seek 0ml
@00:53:39.02 AFV.select :carrierSolution
@00:53:39.14 delay 2 seconds
@00:53:41.16 AS.reconfigure ASconfig
@00:53:41.21 AS.seek 1ml
@00:54:38.94 delay 1 second
@00:54:39.96 AFV.select :holdingCoil
@00:54:40.07 delay 1 second
@00:54:41.09 AS.reconfigure ASfast
@00:54:41.15 AS.seek 0ml
@00:55:08.49 AFV.select :carrierSolution
@00:55:08.61 delay 2 seconds
@00:55:10.63 AS.reconfigure ASconfig
@00:55:10.68 AS.seek 1ml
@00:56:08.44 delay 1 second
@00:56:09.46 AFV.select :holdingCoil
@00:56:09.57 delay 1 second
@00:56:10.59 AS.reconfigure ASfast
@00:56:10.65 AS.seek 0ml
@00:56:37.97 ARV.dial between carrier and air1
@00:56:38.51 ATV.dial :air3
@00:56:39.86 AS.reconfigure ASslow1
@00:56:39.92 AS.seek 0.01ml
@00:56:42.22 delay 2 seconds
@00:56:44.25 ATV.dial :bleach
@00:56:44.91 AS.seek 0.81ml
@00:59:26.16 delay 3 seconds
@00:59:29.19 ATV.dial :air3
@00:59:29.77 delay 3 seconds
@00:59:32.85 AS.seek 0.835ml
@00:59:38.14 delay 3 seconds
@00:59:41.17 ATV.dial :bleach
@00:59:41.81 AS.seek 0.82ml
@00:59:45.55 delay 3 seconds
@00:59:48.58 ATV.dial PCR
@00:59:49.00 AS.reconfigure ASconfig
@00:59:49.06 AS.seek 0ml
@01:00:36.49 PCR.program :bleachProfile
@01:00:36.56 PCR program should complete in < 2:45
@01:00:36.57 PCR.run 10:00
@01:03:38.42 PCR.off
@01:03:38.47 AFV.select :carrierSolution
@01:03:38.58 delay 2 seconds
@01:03:40.60 AS.seek 1ml
@01:04:38.41 delay 1 second
@01:04:39.43 AFV.select :holdingCoil
@01:04:39.54 delay 1 second
@01:04:40.56 AS.reconfigure ASfast
@01:04:40.62 AS.seek 0ml
@01:05:07.97 delay 3 seconds
@01:05:10.99 AFV.select :carrierSolution
@01:05:11.12 delay 2 seconds
@01:05:13.14 AS.reconfigure ASconfig
@01:05:13.19 AS.seek 1ml
@01:06:10.94 delay 1 second
@01:06:11.96 AFV.select :holdingCoil
@01:06:12.07 delay 1 second
@01:06:13.09 AS.reconfigure ASfast
@01:06:13.15 AS.seek 0ml
@01:06:40.50 delay 3 seconds
@01:06:43.52 AFV.select :carrierSolution
@01:06:43.63 delay 2 seconds
@01:06:45.65 AS.reconfigure ASconfig
@01:06:45.75 AS.seek 1ml
@01:07:43.50 delay 1 second
@01:07:44.52 AFV.select :holdingCoil
@01:07:44.63 delay 1 second
@01:07:45.65 AS.reconfigure ASfast
@01:07:45.70 AS.seek 0ml
@01:08:13.05 delay 3 seconds
@01:08:16.07 AFV.select :carrierSolution
@01:08:16.18 delay 2 seconds
@01:08:18.20 AS.reconfigure ASconfig
@01:08:18.26 AS.seek 1ml
@01:09:16.02 delay 1 second
@01:09:17.04 AFV.select :holdingCoil
@01:09:17.15 delay 1 second
@01:09:18.17 AS.reconfigure ASfast
@01:09:18.23 AS.seek 0ml
@01:09:45.58 delay 3 seconds
@01:09:48.60 AFV.select :carrierSolution
@01:09:48.71 delay 2 seconds
@01:09:50.73 AS.reconfigure ASconfig
@01:09:50.79 AS.seek 1ml
@01:10:48.55 delay 1 second
@01:10:49.57 AFV.select :holdingCoil
@01:10:49.68 delay 1 second
@01:10:50.70 AS.reconfigure ASnorm
@01:10:50.75 AS.seek 0ml
@01:11:41.49 AFV.select :carrierSolution
@01:11:41.60 delay 2 seconds
@01:11:43.62 AS.reconfigure ASconfig
@01:11:43.67 AS.seek 1ml
@01:12:41.46 delay 1 second
@01:12:42.49 AFV.select :holdingCoil
@01:12:42.60 delay 1 second
@01:12:43.62 ATV.dial :waste
@01:12:44.89 AS.reconfigure ASnorm
@01:12:44.95 AS.seek 0.5ml
@01:13:10.87 ATV.dial :waste2
@01:13:12.28 AS.seek 0ml
@01:13:37.85 <bspe> Session Ended
@12:56:10.63CEST09-Apr-21 <MAIN> ESP2 v3.30 I2C Gateway v3.06 for core bus
@12:56:13.09 
Configuration for mer 7/20/20 brent@mbari.org
/home/esp/esp2/type/shallow/mer/configure.rb
@12:56:13.17 
Configuration for MFB3 by preston@mbari.org
/home/esp/esp2/type/mfb/MFB3/configure.rb
@12:56:17.86 Polling Can every 10:00
@12:56:19.59 CS.configure :default
@12:56:19.63 CS.hold
@12:56:19.68 CS.configure :default
@12:56:19.76 PS.configure :default
@12:56:19.84 SS.configure :default
@12:56:19.88 SS.hold
@12:56:19.93 SS.configure :default
@12:56:20.01 CC.configure :default
@12:56:20.05 CC.hold
@12:56:20.12 CC.configure :default
@12:56:20.20 PC.configure :default
@12:56:20.24 PC.hold
@12:56:20.30 PC.configure :default
@12:56:20.40 Forearm.configure :loose
@12:56:20.44 Forearm.hold
@12:56:20.48 Forearm.configure :loose
@12:56:20.56 Elbow.configure :loose
@12:56:20.59 Elbow.hold
@12:56:20.64 Elbow.configure :loose
@12:56:20.74 SC.configure :loose
@12:56:20.78 SC.hold
@12:56:20.83 SC.configure :loose
@12:56:20.91 SE.configure :default
@12:56:20.95 SE.hold
@12:56:21.00 SE.configure :default
@12:56:21.04 CH.coast
@12:56:21.08 CH.configure :default
@12:56:21.11 PH.coast
@12:56:21.16 PH.configure :default
@12:56:21.26 SPE.coast
@12:56:21.30 SPE.configure SPEconfig
@12:56:21.37 AS.configure ASconfig
@12:56:21.41 AS.hold
@12:56:21.46 AS.configure ASconfig
@12:56:21.51 PCR.off
@12:56:21.54 PCR.pidtemp= 50,0.1,0,300,25,25
@12:56:21.57 PCR.caltemp= 2.5051,0.0372,0
@12:56:21.60 All dwarf controllers running firmware version 3.75
@12:56:21.79 Cleanly exited at 12:55:56CEST09-Apr-21
@12:56:21.82 Loading /home/esp/esp2/mission/GENOMAP.rb mission
@12:56:21.96 <MFBPCR> Extracting Nucleic Acid from extSrc
@12:56:21.99 SPE.hold 2:46:40,55C
@12:56:22.03 ASV.dial :SPEdelivery
@12:56:22.46 delay 20 seconds
@12:56:23.40 <mwj> Session Begun
@12:56:42.49 <MFBPCR> AFV.select :holdingCoil
@12:56:42.61 ATV.dial :air2
@12:56:43.87 AS.reconfigure ASslow1
@12:56:43.93 AS.seek 0.07ml
@12:56:58.39 delay 3 seconds
@12:57:01.42 ATV.dial :extSrc
@12:57:02.90 AS.seek 0.47ml
@12:58:23.70 delay 5 seconds
@12:58:28.78 Solid Phase Extraction Heater at 39.6C
@12:58:28.79 ATV.dial ASV
@12:58:29.65 ASV.dial :SPEdelivery
@12:58:29.74 AS.seek 0.042ml
@12:59:56.21 delay 10 seconds
@13:00:06.25 Rinsing external lysate line extSrc
@13:00:06.26 AFV.select :carrierSolution
@13:00:06.38 delay 2 seconds
@13:00:08.40 AS.reconfigure ASconfig
@13:00:08.46 AS.seek 1ml
@13:01:03.79 delay 1 second
@13:01:04.81 AFV.select :holdingCoil
@13:01:04.92 delay 1 second
@13:01:05.94 ATV.dial :extSrc
@13:01:06.75 AS.reconfigure ASnorm
@13:01:06.80 AS.seek 0ml
@13:01:57.53 ATV.dial :air2
@13:01:59.12 AS.reconfigure ASslow0
@13:01:59.18 AS.seek 0.05ml
@13:02:04.59 ATV.dial :extSrc
@13:02:06.18 AS.seek 0.01ml
@13:02:10.57 ATV.dial :waste
@13:02:11.93 AS.reconfigure ASconfig
@13:02:11.99 AS.seek 0ml
@13:02:13.23 ethanol wash
@13:02:13.24 ATV.dial :air2
@13:02:14.41 AS.reconfigure ASslow2
@13:02:14.47 AS.seek 0.01ml
@13:02:25.04 delay 3 seconds
@13:02:28.07 Pulling 0.4ml of Ethanol Wash
@13:02:28.08 ATV.dial :ethanol
@13:02:28.96 AS.reconfigure ASslow1
@13:02:29.02 AS.seek 0.41ml
@13:03:49.74 delay 3 seconds
@13:03:52.77 ATV.dial :air1
@13:03:53.18 delay 3 seconds
@13:03:56.25 AS.seek 0.435ml
@13:04:01.63 delay 3 seconds
@13:04:04.66 ATV.dial :ethanol
@13:04:05.29 AS.seek 0.42ml
@13:04:08.60 delay 3 seconds
@13:04:11.63 ATV.dial ASV
@13:04:13.04 AS.seek 0.093ml
@13:05:19.20 delay 3 seconds
@13:05:22.23 ATV.dial :waste2
@13:05:23.05 AS.reconfigure ASconfig
@13:05:23.10 AS.seek 0ml
@13:05:28.76 ATV.dial :air2
@13:05:29.18 AS.reconfigure ASslow1
@13:05:29.23 AS.seek 1ml
@13:08:50.60 delay 5 seconds
@13:08:55.63 ATV.dial ASV
@13:08:56.67 ASV.dial :SPEdelivery
@13:08:56.69 AS.seek 0.02ml
@13:12:14.07 delay 5 seconds
@13:12:19.10 ATV.dial :air2
@13:12:20.20 AS.seek 0.52ml
@13:14:01.09 delay 5 seconds
@13:14:06.12 ATV.dial ASV
@13:14:07.18 ASV.dial :wasteR
@13:14:07.57 AS.seek 0.3ml
@13:14:52.10 delay 20 seconds
@13:15:12.14 AS.seek 0.07ml
@13:15:58.67 delay 20 seconds
@13:16:18.70 ATV.dial :waste2
@13:16:19.50 AS.reconfigure ASconfig
@13:16:19.55 AS.seek 0ml
@13:16:23.87 Elution
@13:16:23.88 ATV.dial :air1
@13:16:25.07 AS.reconfigure ASslow1
@13:16:25.13 AS.seek 0.05ml
@13:16:35.51 delay 3 seconds
@13:16:38.54 ATV.dial :ElutionWater
@13:16:39.26 AS.seek 0.11ml
@13:16:51.73 delay 3 seconds
@13:16:54.76 ATV.dial ASV,via: :air2
@13:16:56.99 ASV.dial :SPEelution
@13:16:57.60 AFV.select :carrierSolution
@13:16:57.76 AS.reconfigure ASnorm
@13:16:57.82 AS.seek 0.76ml
@13:17:30.87 delay 3 seconds
@13:17:33.89 AFV.select :holdingCoil
@13:17:34.04 AS.reconfigure ASslow1
@13:17:34.10 AS.seek 0.475ml
@13:18:31.84 delay 5 seconds
@13:18:36.92 AS.reconfigure ASslow2
@13:18:36.98 AS.seek 0.44ml
@13:19:12.39 delay 1:00
@13:20:12.43 pushing eluate to holding coil
@13:20:12.50 AS.seek 0.28ml
@13:22:53.79 delay 30 seconds
@13:23:23.83 ATV.dial :waste,via: :air3
@13:23:25.66 AS.reconfigure ASconfig
@13:23:25.71 AS.seek 0ml
@13:23:42.10 Parking Bleach in LysateSource line and SPEcolumn
@13:23:42.11 ATV.dial :air3
@13:23:43.02 AS.reconfigure ASslow1
@13:23:43.08 AS.seek 0.03ml
@13:23:49.45 ATV.dial :ElutionWater
@13:23:50.10 AS.seek 0.06ml
@13:23:56.46 ATV.dial :air3
@13:23:57.10 AS.seek 0.07ml
@13:23:59.40 delay 2 seconds
@13:24:01.43 ATV.dial :bleach
@13:24:01.92 AS.seek 0.15ml
@13:24:18.26 delay 3 seconds
@13:24:21.29 ATV.dial :air3
@13:24:21.87 delay 3 seconds
@13:24:24.94 AS.seek 0.175ml
@13:24:30.23 delay 3 seconds
@13:24:33.26 ATV.dial :bleach
@13:24:33.90 AS.seek 0.16ml
@13:24:37.26 delay 3 seconds
@13:24:40.29 ATV.dial :extSrc
@13:24:40.94 AS.reconfigure ASconfig
@13:24:40.99 AS.seek 0.015ml
@13:24:49.63 ATV.dial :waste
@13:24:51.18 AS.seek 0ml
@13:24:52.35 ATV.dial :air3
@13:24:53.23 AS.reconfigure ASslow1
@13:24:53.29 AS.seek 0.01ml
@13:24:55.59 delay 2 seconds
@13:24:57.62 ATV.dial :bleach
@13:24:58.10 AS.seek 0.58ml
@13:26:53.09 delay 3 seconds
@13:26:56.12 ATV.dial :air3
@13:26:56.70 delay 3 seconds
@13:26:59.77 AS.seek 0.605ml
@13:27:05.15 delay 3 seconds
@13:27:08.18 ATV.dial :bleach
@13:27:08.82 AS.seek 0.59ml
@13:27:12.10 delay 3 seconds
@13:27:15.13 ATV.dial :ASV
@13:27:16.21 ASV.dial :wasteR
@13:27:16.83 AS.seek 0ml
@13:29:15.87 delay 1:00
@13:30:15.90 AFV.select :carrierSolution
@13:30:16.04 delay 2 seconds
@13:30:18.07 AS.reconfigure ASconfig
@13:30:18.17 AS.seek 1ml
@13:31:15.96 delay 1 second
@13:31:16.98 AFV.select :holdingCoil
@13:31:17.09 delay 1 second
@13:31:18.11 ATV.dial ASV
@13:31:18.12 ASV.dial :wasteR
@13:31:18.14 AS.reconfigure ASslow1
@13:31:18.20 AS.seek 0ml
@13:34:39.59 delay 20 seconds
@13:34:59.63 ATV.dial :air3
@13:35:01.21 AS.seek 0.59ml
@13:37:00.13 delay 3 seconds
@13:37:03.16 ATV.dial :ASV
@13:37:04.67 ASV.dial :wasteR
@13:37:04.76 AS.seek 0.09ml
@13:38:45.62 delay 30 seconds
@13:39:15.66 ATV.dial :extSrc
@13:39:16.46 delay 3 seconds
@13:39:19.49 ATV.dial :ElutionWater
@13:39:20.65 AS.seek 0.12ml
@13:39:26.99 ATV.dial :air3
@13:39:27.63 AS.seek 0.13ml
@13:39:29.92 delay 2 seconds
@13:39:31.95 ATV.dial :bleach
@13:39:32.43 AS.seek 0.23ml
@13:39:52.94 delay 3 seconds
@13:39:55.98 ATV.dial :air3
@13:39:56.64 delay 3 seconds
@13:39:59.72 AS.seek 0.255ml
@13:40:05.01 delay 3 seconds
@13:40:08.04 ATV.dial :bleach
@13:40:09.17 AS.seek 0.24ml
@13:40:12.53 delay 3 seconds
@13:40:15.56 ATV.dial :ASV
@13:40:16.71 ASV.dial :SPEdelivery
@13:40:17.10 AFV.select :carrierSolution
@13:40:17.21 delay 2 seconds
@13:40:19.23 AS.reconfigure ASconfig
@13:40:19.28 AS.seek 0.5ml
@13:40:34.53 delay 1 second
@13:40:35.55 AFV.select :holdingCoil
@13:40:35.70 AS.reconfigure ASslow1
@13:40:35.76 AS.seek 0.17ml
@13:41:42.44 SPE.hold 2:46:40,95C
@13:41:42.50 delay 3:00
@13:44:42.54 AFV.select :carrierSolution
@13:44:42.66 delay 2 seconds
@13:44:44.68 AS.reconfigure ASconfig
@13:44:44.74 AS.seek 1ml
@13:45:32.75 delay 1 second
@13:45:33.77 AFV.select :holdingCoil
@13:45:33.90 delay 1 second
@13:45:34.94 ASV.dial :wasteD
@13:45:36.07 AS.seek 0ml
@13:46:33.82 Priming Eluate
@13:46:33.83 AFV.select :carrierSolution
@13:46:33.96 delay 2 seconds
@13:46:35.98 AS.seek 0.5ml
@13:47:05.04 delay 1 second
@13:47:06.06 AFV.select :holdingCoil
@13:47:06.17 ATV.dial ASV
@13:47:06.18 ASV.dial :SPErecovery
@13:47:06.82 Swish #1 of 2
@13:47:06.90 AS.reconfigure ASslow0
@13:47:06.96 AS.seek 0.6ml
@13:47:17.41 AS.seek 0.5ml
@13:47:27.76 Swish #2 of 2
@13:47:27.85 AS.seek 0.6ml
@13:47:38.34 AS.seek 0.5ml
@13:47:48.78 delay 3 seconds
@13:47:51.85 AS.reconfigure ASslow1
@13:47:51.91 AS.seek 0.684ml
@13:48:29.32 delay 3 seconds
@13:48:32.35 Eluate primed
@13:48:32.36 ATV.dial :waste2
@13:48:33.04 AS.reconfigure ASnorm
@13:48:33.10 AS.seek 0ml
@13:49:07.85 SPE.coast
@13:49:07.93 ASV.dial :SPErecovery
@13:49:07.96 Setting up PCR for ACYPP2 -- writing /var/log/esp/ACYPP221apr0913h.pcr
@13:49:07.97 Building PCR Slug from ASV with MM1 mix, PP2 primer
@13:49:08.00 Priming with carrier
@13:49:08.02 ATV.dial ARV
@13:49:09.06 ARV.dial :carrier
@13:49:09.41 AS.seek 0.5ml
@13:49:34.93 AFV.select :carrierSolution
@13:49:35.06 delay 2 seconds
@13:49:37.08 AS.reconfigure ASconfig
@13:49:37.14 AS.seek 1ml
@13:50:06.17 delay 1 second
@13:50:07.19 AFV.select :holdingCoil
@13:50:07.30 delay 1 second
@13:50:08.32 ATV.dial :waste
@13:50:09.93 AS.reconfigure ASnorm
@13:50:09.99 AS.seek 0ml
@13:51:00.65 AFV.select :carrierSolution
@13:51:00.78 delay 2 seconds
@13:51:02.80 AS.reconfigure ASconfig
@13:51:02.86 AS.seek 0.5ml
@13:51:31.90 delay 1 second
@13:51:32.92 AFV.select :holdingCoil
@13:51:33.03 Building Slug
@13:51:33.04 ATV.dial :ARV
@13:51:34.79 delay 2 seconds
@13:51:36.82 ARV.dial :air1
@13:51:37.21 delay 2 seconds
@13:51:39.28 AS.reconfigure ASslow2
@13:51:39.34 AS.seek 0.506ml
@13:51:45.70 delay 2 seconds
@13:51:47.73 ARV.dial :MM1
@13:51:48.37 delay 2 seconds
@13:51:50.40 Adding MM1
@13:51:50.47 AS.seek 0.524ml
@13:52:09.01 delay 25 seconds
@13:52:34.04 ARV.dial :PP2
@13:52:34.46 Adding PP2
@13:52:34.47 delay 2 seconds
@13:52:36.55 AS.seek 0.53ml
@13:52:42.71 delay 10 seconds
@13:52:52.75 ARV.dial :air2
@13:52:53.18 adding ARV air
@13:52:53.19 delay 2 seconds
@13:52:55.27 AS.seek 0.536ml
@13:53:01.51 delay 5 seconds
@13:53:06.54 ARV.dial :carrier
@13:53:07.90 delay 7 seconds
@13:53:14.99 AS.seek 0.594ml
@13:54:13.43 delay 5 seconds
@13:54:18.46 ATV.dial :ASV
@13:54:18.88 delay 2 seconds
@13:54:20.96 AS.seek 0.6ml
@13:54:27.37 delay 4 seconds
@13:54:31.40 ATV.dial ARV
@13:54:31.98 delay 2 seconds
@13:54:34.01 ARV.dial :carrier
@13:54:34.02 delay 2 seconds
@13:54:36.10 AS.seek 0.65ml
@13:55:26.93 delay 4 seconds
@13:55:30.96 ATV.dial PCR
@13:55:31.59 ASV.dial :waste1
@13:55:32.40 ARV.dial :air1
@13:55:32.83 Positioning Slug by 0.324ml
@13:55:32.91 AS.seek 0.326ml
@14:00:59.24 delay 5 seconds
@14:01:04.27 ATV.dial ARV
@14:01:05.07 ASV.dial :PCRlock
@14:01:05.46 delay 5 seconds
@14:01:10.49 Running ACYPP2 Profile60CIQ2 thermocycling
@14:01:10.50 <PCR> PCR.program Profile60CIQ2
@14:01:10.62 Profile60CIQ2 should complete in < 1:47:50
@14:01:10.63 PCR.run 2:19:47.5
@15:30:13.09 PCR.off
@15:30:13.74 <MFBPCR> Running ACYPP2 MeltProfile60 thermocycling
@15:30:13.75 <PCR> PCR.program MeltProfile60
@15:30:13.86 MeltProfile60 should complete in < 11:55
@15:30:13.87 PCR.run 19:53.75
@15:38:03.83 PCR.off
@15:38:04.78 <MFBPCR> PCR for ACYPP2 completed -- closed /var/log/esp/ACYPP221apr0913h.pcr
@15:38:04.85 Cleaning PCR
@15:38:04.86 AFV.select :carrierSolution
@15:38:04.98 delay 2 seconds
@15:38:07.00 AS.reconfigure ASconfig
@15:38:07.05 AS.seek 0.5ml
@15:38:17.37 delay 1 second
@15:38:18.39 AFV.select :holdingCoil
@15:38:18.50 ASV.dial :waste1
@15:38:18.90 ATV.dial :air3
@15:38:20.26 AS.reconfigure ASslow1
@15:38:20.32 AS.seek 0.51ml
@15:38:22.60 delay 2 seconds
@15:38:24.62 ATV.dial :bleach
@15:38:25.26 AS.seek 0.81ml
@15:39:25.85 delay 3 seconds
@15:39:28.88 ATV.dial :air3
@15:39:29.46 delay 3 seconds
@15:39:32.54 AS.seek 0.835ml
@15:39:37.96 delay 3 seconds
@15:39:40.99 ATV.dial :bleach
@15:39:41.63 AS.seek 0.82ml
@15:39:44.85 delay 3 seconds
@15:39:47.88 ARV.dial :waste
@15:39:48.52 delay 3 seconds
@15:39:51.55 ATV.dial :ARV
@15:39:52.47 AS.reconfigure ASconfig
@15:39:52.53 AS.seek 0.47ml
@15:40:12.93 AFV.select :carrierSolution
@15:40:13.05 delay 2 seconds
@15:40:15.07 AS.seek 1ml
@15:40:45.84 delay 1 second
@15:40:46.86 AFV.select :holdingCoil
@15:40:46.97 delay 1 second
@15:40:47.99 AS.reconfigure ASfast
@15:40:48.04 AS.seek 0ml
@15:41:15.37 AFV.select :carrierSolution
@15:41:15.49 delay 2 seconds
@15:41:17.51 AS.reconfigure ASconfig
@15:41:17.57 AS.seek 1ml
@15:42:15.34 delay 1 second
@15:42:16.36 AFV.select :holdingCoil
@15:42:16.47 delay 1 second
@15:42:17.49 AS.reconfigure ASfast
@15:42:17.55 AS.seek 0ml
@15:42:44.87 AFV.select :carrierSolution
@15:42:44.99 delay 2 seconds
@15:42:47.01 AS.reconfigure ASconfig
@15:42:47.06 AS.seek 1ml
@15:43:44.82 delay 1 second
@15:43:45.84 AFV.select :holdingCoil
@15:43:45.95 delay 1 second
@15:43:46.97 AS.reconfigure ASfast
@15:43:47.02 AS.seek 0ml
@15:44:14.35 ARV.dial between carrier and air1
@15:44:14.88 ATV.dial :air3
@15:44:16.21 AS.reconfigure ASslow1
@15:44:16.27 AS.seek 0.01ml
@15:44:18.66 delay 2 seconds
@15:44:20.69 ATV.dial :bleach
@15:44:21.33 AS.seek 0.81ml
@15:47:02.48 delay 3 seconds
@15:47:05.51 ATV.dial :air3
@15:47:06.09 delay 3 seconds
@15:47:09.16 AS.seek 0.835ml
@15:47:14.46 delay 3 seconds
@15:47:17.49 ATV.dial :bleach
@15:47:18.13 AS.seek 0.82ml
@15:47:21.40 delay 3 seconds
@15:47:24.43 ATV.dial PCR
@15:47:24.85 AS.reconfigure ASconfig
@15:47:24.90 AS.seek 0ml
@15:48:12.30 PCR.program :bleachProfile
@15:48:12.37 PCR program should complete in < 2:45
@15:48:12.39 PCR.run 10:00
@15:51:08.61 PCR.off
@15:51:08.65 AFV.select :carrierSolution
@15:51:08.76 delay 2 seconds
@15:51:10.78 AS.seek 1ml
@15:52:08.54 delay 1 second
@15:52:09.56 AFV.select :holdingCoil
@15:52:09.67 delay 1 second
@15:52:10.69 AS.reconfigure ASfast
@15:52:10.74 AS.seek 0ml
@15:52:38.05 delay 3 seconds
@15:52:41.07 AFV.select :carrierSolution
@15:52:41.18 delay 2 seconds
@15:52:43.20 AS.reconfigure ASconfig
@15:52:43.27 AS.seek 1ml
@15:53:41.02 delay 1 second
@15:53:42.04 AFV.select :holdingCoil
@15:53:42.15 delay 1 second
@15:53:43.17 AS.reconfigure ASfast
@15:53:43.22 AS.seek 0ml
@15:54:10.55 delay 3 seconds
@15:54:13.57 AFV.select :carrierSolution
@15:54:13.68 delay 2 seconds
@15:54:15.70 AS.reconfigure ASconfig
@15:54:15.76 AS.seek 1ml
@15:55:13.52 delay 1 second
@15:55:14.54 AFV.select :holdingCoil
@15:55:14.65 delay 1 second
@15:55:15.67 AS.reconfigure ASfast
@15:55:15.73 AS.seek 0ml
@15:55:43.07 delay 3 seconds
@15:55:46.09 AFV.select :carrierSolution
@15:55:46.20 delay 2 seconds
@15:55:48.22 AS.reconfigure ASconfig
@15:55:48.29 AS.seek 1ml
@15:56:46.09 delay 1 second
@15:56:47.11 AFV.select :holdingCoil
@15:56:47.22 delay 1 second
@15:56:48.24 AS.reconfigure ASfast
@15:56:48.30 AS.seek 0ml
@15:57:15.63 delay 3 seconds
@15:57:18.65 AFV.select :carrierSolution
@15:57:18.76 delay 2 seconds
@15:57:20.78 AS.reconfigure ASconfig
@15:57:20.85 AS.seek 1ml
@15:58:18.60 delay 1 second
@15:58:19.62 AFV.select :holdingCoil
@15:58:19.73 delay 1 second
@15:58:20.75 AS.reconfigure ASnorm
@15:58:20.80 AS.seek 0ml
@15:59:11.55 Setting up PCR for AOPP1 -- writing /var/log/esp/AOPP121apr0915h.pcr
@15:59:11.57 Building PCR Slug from ASV with MM1 mix, PP1 primer
@15:59:11.59 Priming with carrier
@15:59:11.61 ATV.dial ARV
@15:59:12.35 ARV.dial :carrier
@15:59:12.71 AS.seek 0.5ml
@15:59:38.26 AFV.select :carrierSolution
@15:59:38.39 delay 2 seconds
@15:59:40.41 AS.reconfigure ASconfig
@15:59:40.47 AS.seek 1ml
@16:00:09.48 delay 1 second
@16:00:10.50 AFV.select :holdingCoil
@16:00:10.61 delay 1 second
@16:00:11.63 ATV.dial :waste
@16:00:13.39 AS.reconfigure ASnorm
@16:00:13.45 AS.seek 0ml
@16:01:04.12 AFV.select :carrierSolution
@16:01:04.24 delay 2 seconds
@16:01:06.26 AS.reconfigure ASconfig
@16:01:06.32 AS.seek 0.5ml
@16:01:35.37 delay 1 second
@16:01:36.39 AFV.select :holdingCoil
@16:01:36.50 Building Slug
@16:01:36.51 ATV.dial :ARV
@16:01:38.26 delay 2 seconds
@16:01:40.29 ARV.dial :air1
@16:01:40.69 delay 2 seconds
@16:01:42.76 AS.reconfigure ASslow2
@16:01:42.83 AS.seek 0.506ml
@16:01:49.16 delay 2 seconds
@16:01:51.19 ARV.dial :MM1
@16:01:51.82 delay 2 seconds
@16:01:53.85 Adding MM1
@16:01:53.92 AS.seek 0.524ml
@16:02:12.41 delay 25 seconds
@16:02:37.44 ARV.dial :PP1
@16:02:37.85 Adding PP1
@16:02:37.86 delay 2 seconds
@16:02:39.94 AS.seek 0.53ml
@16:02:46.51 delay 10 seconds
@16:02:56.55 ARV.dial :air1
@16:02:56.97 adding ARV air
@16:02:56.98 delay 2 seconds
@16:02:59.06 AS.seek 0.536ml
@16:03:05.30 delay 5 seconds
@16:03:10.33 ARV.dial :carrier
@16:03:10.74 delay 7 seconds
@16:03:17.82 AS.seek 0.594ml
@16:04:16.43 delay 5 seconds
@16:04:21.46 ATV.dial :ASV
@16:04:21.88 delay 2 seconds
@16:04:23.95 AS.seek 0.6ml
@16:04:30.21 delay 4 seconds
@16:04:34.24 ATV.dial ARV
@16:04:35.27 delay 2 seconds
@16:04:37.30 ARV.dial :carrier
@16:04:37.31 delay 2 seconds
@16:04:39.39 AS.seek 0.65ml
@16:05:30.21 delay 4 seconds
@16:05:34.24 ATV.dial PCR
@16:05:34.87 ASV.dial :waste1
@16:05:34.89 ARV.dial :air1
@16:05:35.31 Positioning Slug by 0.324ml
@16:05:35.40 AS.seek 0.326ml
@16:11:02.10 delay 5 seconds
@16:11:07.13 ATV.dial ARV
@16:11:07.93 ASV.dial :PCRlock
@16:11:08.34 delay 5 seconds
@16:11:13.37 Running AOPP1 Profile60CIQ1 thermocycling
@16:11:13.38 <PCR> PCR.program Profile60CIQ1
@16:11:13.50 Profile60CIQ1 should complete in < 1:56:15
@16:11:13.52 PCR.run 2:30:18.75
@17:50:49.22 PCR.off
@17:50:49.80 <MFBPCR> Running AOPP1 MeltProfile60 thermocycling
@17:50:49.82 <PCR> PCR.program MeltProfile60
@17:50:49.92 MeltProfile60 should complete in < 11:55
@17:50:49.93 PCR.run 19:53.75
@17:58:30.82 PCR.off
@17:58:31.76 <MFBPCR> PCR for AOPP1 completed -- closed /var/log/esp/AOPP121apr0915h.pcr
@17:58:31.83 Cleaning PCR
@17:58:31.84 AFV.select :carrierSolution
@17:58:31.96 delay 2 seconds
@17:58:33.98 AS.reconfigure ASconfig
@17:58:34.04 AS.seek 0.5ml
@17:58:44.40 delay 1 second
@17:58:45.43 AFV.select :holdingCoil
@17:58:45.54 ASV.dial :waste1
@17:58:45.93 ATV.dial :air3
@17:58:47.28 AS.reconfigure ASslow1
@17:58:47.34 AS.seek 0.51ml
@17:58:49.70 delay 2 seconds
@17:58:51.73 ATV.dial :bleach
@17:58:52.36 AS.seek 0.81ml
@17:59:52.96 delay 3 seconds
@17:59:55.99 ATV.dial :air3
@17:59:56.57 delay 3 seconds
@17:59:59.64 AS.seek 0.835ml
@18:00:04.94 delay 3 seconds
@18:00:07.97 ATV.dial :bleach
@18:00:08.61 AS.seek 0.82ml
@18:00:11.94 delay 3 seconds
@18:00:14.97 ARV.dial :waste
@18:00:15.60 delay 3 seconds
@18:00:18.63 ATV.dial :ARV
@18:00:19.55 AS.reconfigure ASconfig
@18:00:19.61 AS.seek 0.47ml
@18:00:40.01 AFV.select :carrierSolution
@18:00:40.13 delay 2 seconds
@18:00:42.15 AS.seek 1ml
@18:01:12.90 delay 1 second
@18:01:13.92 AFV.select :holdingCoil
@18:01:14.03 delay 1 second
@18:01:15.05 AS.reconfigure ASfast
@18:01:15.11 AS.seek 0ml
@18:01:42.46 AFV.select :carrierSolution
@18:01:42.58 delay 2 seconds
@18:01:44.60 AS.reconfigure ASconfig
@18:01:44.65 AS.seek 1ml
@18:02:42.44 delay 1 second
@18:02:43.46 AFV.select :holdingCoil
@18:02:43.57 delay 1 second
@18:02:44.59 AS.reconfigure ASfast
@18:02:44.64 AS.seek 0ml
@18:03:11.97 AFV.select :carrierSolution
@18:03:12.09 delay 2 seconds
@18:03:14.11 AS.reconfigure ASconfig
@18:03:14.16 AS.seek 1ml
@18:04:11.91 delay 1 second
@18:04:12.93 AFV.select :holdingCoil
@18:04:13.04 delay 1 second
@18:04:14.06 AS.reconfigure ASfast
@18:04:14.11 AS.seek 0ml
@18:04:41.46 ARV.dial between carrier and air1
@18:04:41.97 ATV.dial :air3
@18:04:43.30 AS.reconfigure ASslow1
@18:04:43.36 AS.seek 0.01ml
@18:04:45.66 delay 2 seconds
@18:04:47.69 ATV.dial :bleach
@18:04:48.33 AS.seek 0.81ml
@18:07:29.54 delay 3 seconds
@18:07:32.57 ATV.dial :air3
@18:07:33.15 delay 3 seconds
@18:07:36.22 AS.seek 0.835ml
@18:07:41.52 delay 3 seconds
@18:07:44.55 ATV.dial :bleach
@18:07:45.19 AS.seek 0.82ml
@18:07:48.62 delay 3 seconds
@18:07:51.65 ATV.dial PCR
@18:07:52.06 AS.reconfigure ASconfig
@18:07:52.11 AS.seek 0ml
@18:08:39.54 PCR.program :bleachProfile
@18:08:40.03 PCR program should complete in < 2:45
@18:08:40.04 PCR.run 10:00
@18:11:35.84 PCR.off
@18:11:35.88 AFV.select :carrierSolution
@18:11:35.99 delay 2 seconds
@18:11:38.01 AS.seek 1ml
@18:12:35.77 delay 1 second
@18:12:36.79 AFV.select :holdingCoil
@18:12:36.90 delay 1 second
@18:12:37.92 AS.reconfigure ASfast
@18:12:37.97 AS.seek 0ml
@18:13:05.30 delay 3 seconds
@18:13:08.32 AFV.select :carrierSolution
@18:13:08.43 delay 2 seconds
@18:13:10.45 AS.reconfigure ASconfig
@18:13:10.51 AS.seek 1ml
@18:14:08.27 delay 1 second
@18:14:09.29 AFV.select :holdingCoil
@18:14:09.40 delay 1 second
@18:14:10.42 AS.reconfigure ASfast
@18:14:10.47 AS.seek 0ml
@18:14:37.80 delay 3 seconds
@18:14:40.82 AFV.select :carrierSolution
@18:14:40.93 delay 2 seconds
@18:14:42.95 AS.reconfigure ASconfig
@18:14:43.02 AS.seek 1ml
@18:15:40.79 delay 1 second
@18:15:41.81 AFV.select :holdingCoil
@18:15:41.92 delay 1 second
@18:15:42.94 AS.reconfigure ASfast
@18:15:43.00 AS.seek 0ml
@18:16:10.33 delay 3 seconds
@18:16:13.35 AFV.select :carrierSolution
@18:16:13.46 delay 2 seconds
@18:16:15.48 AS.reconfigure ASconfig
@18:16:15.55 AS.seek 1ml
@18:17:13.30 delay 1 second
@18:17:14.32 AFV.select :holdingCoil
@18:17:14.43 delay 1 second
@18:17:15.45 AS.reconfigure ASfast
@18:17:15.50 AS.seek 0ml
@18:17:42.85 delay 3 seconds
@18:17:45.87 AFV.select :carrierSolution
@18:17:45.98 delay 2 seconds
@18:17:48.00 AS.reconfigure ASconfig
@18:17:48.07 AS.seek 1ml
@18:18:45.91 delay 1 second
@18:18:46.98 AFV.select :holdingCoil
@18:18:47.20 delay 1 second
@18:18:48.30 AS.reconfigure ASnorm
@18:18:48.47 AS.seek 0ml
@18:19:39.34 Setting up PCR for AARPP4 -- writing /var/log/esp/AARPP421apr0918h.pcr
@18:19:39.36 Building PCR Slug from ASV with MM2 mix, PP4 primer
@18:19:39.38 Priming with carrier
@18:19:39.40 ATV.dial ARV
@18:19:40.05 ARV.dial :carrier
@18:19:40.39 AS.seek 0.5ml
@18:20:05.93 AFV.select :carrierSolution
@18:20:06.06 delay 2 seconds
@18:20:08.08 AS.reconfigure ASconfig
@18:20:08.14 AS.seek 1ml
@18:20:37.16 delay 1 second
@18:20:38.18 AFV.select :holdingCoil
@18:20:38.29 delay 1 second
@18:20:39.31 ATV.dial :waste
@18:20:41.08 AS.reconfigure ASnorm
@18:20:41.14 AS.seek 0ml
@18:21:31.82 AFV.select :carrierSolution
@18:21:31.94 delay 2 seconds
@18:21:33.96 AS.reconfigure ASconfig
@18:21:34.43 AS.seek 0.5ml
@18:22:03.49 delay 1 second
@18:22:04.51 AFV.select :holdingCoil
@18:22:04.62 Building Slug
@18:22:04.63 ATV.dial :ARV
@18:22:06.37 delay 2 seconds
@18:22:08.40 ARV.dial :air1
@18:22:08.81 delay 2 seconds
@18:22:10.88 AS.reconfigure ASslow2
@18:22:10.94 AS.seek 0.506ml
@18:22:17.19 delay 2 seconds
@18:22:19.22 ARV.dial :MM2
@18:22:20.80 delay 2 seconds
@18:22:22.83 Adding MM2
@18:22:22.91 AS.seek 0.524ml
@18:22:41.13 delay 25 seconds
@18:23:06.16 ARV.dial :PP4
@18:23:06.59 Adding PP4
@18:23:06.60 delay 2 seconds
@18:23:08.67 AS.seek 0.53ml
@18:23:15.05 delay 10 seconds
@18:23:25.09 ARV.dial :air3
@18:23:25.49 adding ARV air
@18:23:25.51 delay 2 seconds
@18:23:27.58 AS.seek 0.536ml
@18:23:34.27 delay 5 seconds
@18:23:39.30 ARV.dial :carrier
@18:23:40.62 delay 7 seconds
@18:23:47.71 AS.seek 0.594ml
@18:24:46.35 delay 5 seconds
@18:24:51.38 ATV.dial :ASV
@18:24:51.80 delay 2 seconds
@18:24:53.88 AS.seek 0.6ml
@18:25:00.32 delay 4 seconds
@18:25:04.35 ATV.dial ARV
@18:25:04.93 delay 2 seconds
@18:25:06.96 ARV.dial :carrier
@18:25:06.97 delay 2 seconds
@18:25:09.05 AS.seek 0.65ml
@18:25:59.66 delay 4 seconds
@18:26:03.69 ATV.dial PCR
@18:26:04.32 ASV.dial :waste1
@18:26:04.34 ARV.dial :air1
@18:26:04.76 Positioning Slug by 0.324ml
@18:26:04.84 AS.seek 0.326ml
@18:31:31.43 delay 5 seconds
@18:31:36.46 ATV.dial ARV
@18:31:37.26 ASV.dial :PCRlock
@18:31:37.66 delay 5 seconds
@18:31:42.69 Running AARPP4 Profile58CIQ2 thermocycling
@18:31:42.70 <PCR> PCR.program Profile58CIQ2
@18:31:42.83 Profile58CIQ2 should complete in < 1:36:15
@18:31:42.84 PCR.run 2:05:18.75
@19:52:48.87 PCR.off
@19:52:49.44 <MFBPCR> Running AARPP4 MeltProfile58 thermocycling
@19:52:49.46 <PCR> PCR.program MeltProfile58
@19:52:49.56 MeltProfile58 should complete in < 12:33
@19:52:49.57 PCR.run 20:41.25
@20:00:34.31 PCR.off
@20:00:35.31 <MFBPCR> PCR for AARPP4 completed -- closed /var/log/esp/AARPP421apr0918h.pcr
@20:00:35.38 Cleaning PCR
@20:00:35.39 AFV.select :carrierSolution
@20:00:35.51 delay 2 seconds
@20:00:37.53 AS.reconfigure ASconfig
@20:00:37.58 AS.seek 0.5ml
@20:00:47.93 delay 1 second
@20:00:48.95 AFV.select :holdingCoil
@20:00:49.06 ASV.dial :waste1
@20:00:49.46 ATV.dial :air3
@20:00:50.82 AS.reconfigure ASslow1
@20:00:50.88 AS.seek 0.51ml
@20:00:53.13 delay 2 seconds
@20:00:55.16 ATV.dial :bleach
@20:00:55.80 AS.seek 0.81ml
@20:01:56.38 delay 3 seconds
@20:01:59.41 ATV.dial :air3
@20:01:59.99 delay 3 seconds
@20:02:03.06 AS.seek 0.835ml
@20:02:08.37 delay 3 seconds
@20:02:11.40 ATV.dial :bleach
@20:02:12.03 AS.seek 0.82ml
@20:02:15.32 delay 3 seconds
@20:02:18.35 ARV.dial :waste
@20:02:18.97 delay 3 seconds
@20:02:22.00 ATV.dial :ARV
@20:02:22.92 AS.reconfigure ASconfig
@20:02:22.97 AS.seek 0.47ml
@20:02:43.36 AFV.select :carrierSolution
@20:02:43.47 delay 2 seconds
@20:02:45.89 AS.seek 1ml
@20:03:16.66 delay 1 second
@20:03:17.68 AFV.select :holdingCoil
@20:03:17.79 delay 1 second
@20:03:18.81 AS.reconfigure ASfast
@20:03:18.87 AS.seek 0ml
@20:03:46.21 AFV.select :carrierSolution
@20:03:46.33 delay 2 seconds
@20:03:48.35 AS.reconfigure ASconfig
@20:03:48.41 AS.seek 1ml
@20:04:46.16 delay 1 second
@20:04:47.18 AFV.select :holdingCoil
@20:04:47.29 delay 1 second
@20:04:48.31 AS.reconfigure ASfast
@20:04:48.37 AS.seek 0ml
@20:05:15.72 AFV.select :carrierSolution
@20:05:15.84 delay 2 seconds
@20:05:17.86 AS.reconfigure ASconfig
@20:05:17.94 AS.seek 1ml
@20:06:15.71 delay 1 second
@20:06:16.73 AFV.select :holdingCoil
@20:06:16.84 delay 1 second
@20:06:17.86 AS.reconfigure ASfast
@20:06:17.92 AS.seek 0ml
@20:06:45.27 ARV.dial between carrier and air1
@20:06:45.81 ATV.dial :air3
@20:06:47.14 AS.reconfigure ASslow1
@20:06:47.20 AS.seek 0.01ml
@20:06:49.60 delay 2 seconds
@20:06:51.63 ATV.dial :bleach
@20:06:52.27 AS.seek 0.81ml
@20:09:33.47 delay 3 seconds
@20:09:36.50 ATV.dial :air3
@20:09:37.08 delay 3 seconds
@20:09:40.15 AS.seek 0.835ml
@20:09:45.45 delay 3 seconds
@20:09:48.48 ATV.dial :bleach
@20:09:49.13 AS.seek 0.82ml
@20:09:52.57 delay 3 seconds
@20:09:55.60 ATV.dial PCR
@20:09:56.02 AS.reconfigure ASconfig
@20:09:56.07 AS.seek 0ml
@20:10:43.47 PCR.program :bleachProfile
@20:10:43.54 PCR program should complete in < 2:45
@20:10:43.55 PCR.run 10:00
@20:13:39.36 PCR.off
@20:13:39.41 AFV.select :carrierSolution
@20:13:39.52 delay 2 seconds
@20:13:41.54 AS.seek 1ml
@20:14:39.30 delay 1 second
@20:14:40.32 AFV.select :holdingCoil
@20:14:40.43 delay 1 second
@20:14:41.45 AS.reconfigure ASfast
@20:14:41.51 AS.seek 0ml
@20:15:08.86 delay 3 seconds
@20:15:11.88 AFV.select :carrierSolution
@20:15:11.99 delay 2 seconds
@20:15:14.01 AS.reconfigure ASconfig
@20:15:14.07 AS.seek 1ml
@20:16:11.82 delay 1 second
@20:16:12.84 AFV.select :holdingCoil
@20:16:12.95 delay 1 second
@20:16:13.97 AS.reconfigure ASfast
@20:16:14.02 AS.seek 0ml
@20:16:41.44 delay 3 seconds
@20:16:44.45 AFV.select :carrierSolution
@20:16:44.56 delay 2 seconds
@20:16:46.58 AS.reconfigure ASconfig
@20:16:46.63 AS.seek 1ml
@20:17:44.36 delay 1 second
@20:17:45.38 AFV.select :holdingCoil
@20:17:45.49 delay 1 second
@20:17:46.51 AS.reconfigure ASfast
@20:17:46.57 AS.seek 0ml
@20:18:13.93 delay 3 seconds
@20:18:16.95 AFV.select :carrierSolution
@20:18:17.06 delay 2 seconds
@20:18:19.08 AS.reconfigure ASconfig
@20:18:19.14 AS.seek 1ml
@20:19:16.90 delay 1 second
@20:19:17.92 AFV.select :holdingCoil
@20:19:18.03 delay 1 second
@20:19:19.05 AS.reconfigure ASfast
@20:19:19.11 AS.seek 0ml
@20:19:46.46 delay 3 seconds
@20:19:49.48 AFV.select :carrierSolution
@20:19:49.59 delay 2 seconds
@20:19:51.61 AS.reconfigure ASconfig
@20:19:51.66 AS.seek 1ml
@20:20:49.47 delay 1 second
@20:20:50.49 AFV.select :holdingCoil
@20:20:50.60 delay 1 second
@20:20:51.62 AS.reconfigure ASnorm
@20:20:51.68 AS.seek 0ml
@20:21:42.44 Setting up PCR for AALPP3 -- writing /var/log/esp/AALPP321apr0920h.pcr
@20:21:42.46 Building PCR Slug from ASV with MM2 mix, PP3 primer
@20:21:42.48 Priming with carrier
@20:21:42.49 ATV.dial ARV
@20:21:43.15 ARV.dial :carrier
@20:21:43.50 AS.seek 0.5ml
@20:22:09.04 AFV.select :carrierSolution
@20:22:09.17 delay 2 seconds
@20:22:11.19 AS.reconfigure ASconfig
@20:22:11.26 AS.seek 1ml
@20:22:40.29 delay 1 second
@20:22:41.31 AFV.select :holdingCoil
@20:22:41.42 delay 1 second
@20:22:42.44 ATV.dial :waste
@20:22:44.20 AS.reconfigure ASnorm
@20:22:44.26 AS.seek 0ml
@20:23:34.96 AFV.select :carrierSolution
@20:23:35.09 delay 2 seconds
@20:23:37.11 AS.reconfigure ASconfig
@20:23:37.17 AS.seek 0.5ml
@20:24:06.22 delay 1 second
@20:24:07.24 AFV.select :holdingCoil
@20:24:07.35 Building Slug
@20:24:07.36 ATV.dial :ARV
@20:24:09.10 delay 2 seconds
@20:24:11.13 ARV.dial :air1
@20:24:11.53 delay 2 seconds
@20:24:13.60 AS.reconfigure ASslow2
@20:24:13.66 AS.seek 0.506ml
@20:24:20.15 delay 2 seconds
@20:24:22.18 ARV.dial :MM2
@20:24:23.76 delay 2 seconds
@20:24:25.79 Adding MM2
@20:24:25.87 AS.seek 0.524ml
@20:24:44.19 delay 25 seconds
@20:25:09.22 ARV.dial :PP3
@20:25:09.61 Adding PP3
@20:25:09.63 delay 2 seconds
@20:25:11.70 AS.seek 0.53ml
@20:25:18.07 delay 10 seconds
@20:25:28.11 ARV.dial :air2
@20:25:28.55 adding ARV air
@20:25:28.56 delay 2 seconds
@20:25:30.63 AS.seek 0.536ml
@20:25:36.93 delay 5 seconds
@20:25:41.96 ARV.dial :carrier
@20:25:43.30 delay 7 seconds
@20:25:50.38 AS.seek 0.594ml
@20:26:48.86 delay 5 seconds
@20:26:53.89 ATV.dial :ASV
@20:26:54.30 delay 2 seconds
@20:26:56.37 AS.seek 0.6ml
@20:27:02.75 delay 4 seconds
@20:27:06.78 ATV.dial ARV
@20:27:07.36 delay 2 seconds
@20:27:09.39 ARV.dial :carrier
@20:27:09.40 delay 2 seconds
@20:27:11.47 AS.seek 0.65ml
@20:28:02.10 delay 4 seconds
@20:28:06.13 ATV.dial PCR
@20:28:06.76 ASV.dial :waste1
@20:28:06.78 ARV.dial :air1
@20:28:07.20 Positioning Slug by 0.324ml
@20:28:07.28 AS.seek 0.326ml
@20:33:33.29 delay 5 seconds
@20:33:38.32 ATV.dial ARV
@20:33:39.13 ASV.dial :PCRlock
@20:33:39.54 delay 5 seconds
@20:33:44.57 Running AALPP3 Profile58CIQ2 thermocycling
@20:33:44.58 <PCR> PCR.program Profile58CIQ2
@20:33:44.71 Profile58CIQ2 should complete in < 1:36:15
@20:33:44.72 PCR.run 2:05:18.75
@21:54:45.99 PCR.off
@21:54:46.56 <MFBPCR> Running AALPP3 MeltProfile58 thermocycling
@21:54:46.58 <PCR> PCR.program MeltProfile58
@21:54:46.68 MeltProfile58 should complete in < 12:33
@21:54:46.69 PCR.run 20:41.25
@22:02:33.93 PCR.off
@22:02:34.94 <MFBPCR> PCR for AALPP3 completed -- closed /var/log/esp/AALPP321apr0920h.pcr
@22:02:35.01 Cleaning PCR
@22:02:35.02 AFV.select :carrierSolution
@22:02:35.15 delay 2 seconds
@22:02:37.17 AS.reconfigure ASconfig
@22:02:37.23 AS.seek 0.5ml
@22:02:47.52 delay 1 second
@22:02:48.54 AFV.select :holdingCoil
@22:02:48.65 ASV.dial :waste1
@22:02:49.04 ATV.dial :air3
@22:02:50.39 AS.reconfigure ASslow1
@22:02:50.45 AS.seek 0.51ml
@22:02:52.75 delay 2 seconds
@22:02:54.78 ATV.dial :bleach
@22:02:55.42 AS.seek 0.81ml
@22:03:56.01 delay 3 seconds
@22:03:59.04 ATV.dial :air3
@22:03:59.61 delay 3 seconds
@22:04:02.68 AS.seek 0.835ml
@22:04:08.00 delay 3 seconds
@22:04:11.03 ATV.dial :bleach
@22:04:11.67 AS.seek 0.82ml
@22:04:14.99 delay 3 seconds
@22:04:18.02 ARV.dial :waste
@22:04:18.66 delay 3 seconds
@22:04:21.69 ATV.dial :ARV
@22:04:22.63 AS.reconfigure ASconfig
@22:04:22.68 AS.seek 0.47ml
@22:04:43.10 AFV.select :carrierSolution
@22:04:43.21 delay 2 seconds
@22:04:45.23 AS.seek 1ml
@22:05:15.97 delay 1 second
@22:05:16.99 AFV.select :holdingCoil
@22:05:17.10 delay 1 second
@22:05:18.12 AS.reconfigure ASfast
@22:05:18.18 AS.seek 0ml
@22:05:45.47 AFV.select :carrierSolution
@22:05:45.59 delay 2 seconds
@22:05:47.61 AS.reconfigure ASconfig
@22:05:47.66 AS.seek 1ml
@22:06:45.41 delay 1 second
@22:06:46.43 AFV.select :holdingCoil
@22:06:46.54 delay 1 second
@22:06:47.56 AS.reconfigure ASfast
@22:06:47.61 AS.seek 0ml
@22:07:14.94 AFV.select :carrierSolution
@22:07:15.06 delay 2 seconds
@22:07:17.08 AS.reconfigure ASconfig
@22:07:17.13 AS.seek 1ml
@22:08:14.86 delay 1 second
@22:08:15.88 AFV.select :holdingCoil
@22:08:15.99 delay 1 second
@22:08:17.01 AS.reconfigure ASfast
@22:08:17.07 AS.seek 0ml
@22:08:44.41 ARV.dial between carrier and air1
@22:08:44.94 ATV.dial :air3
@22:08:46.30 AS.reconfigure ASslow1
@22:08:46.36 AS.seek 0.01ml
@22:08:48.68 delay 2 seconds
@22:08:50.71 ATV.dial :bleach
@22:08:51.35 AS.seek 0.81ml
@22:11:32.58 delay 3 seconds
@22:11:35.61 ATV.dial :air3
@22:11:36.19 delay 3 seconds
@22:11:39.26 AS.seek 0.835ml
@22:11:44.58 delay 3 seconds
@22:11:47.61 ATV.dial :bleach
@22:11:48.25 AS.seek 0.82ml
@22:11:51.55 delay 3 seconds
@22:11:54.58 ATV.dial PCR
@22:11:55.02 AS.reconfigure ASconfig
@22:11:55.07 AS.seek 0ml
@22:12:42.51 PCR.program :bleachProfile
@22:12:42.59 PCR program should complete in < 2:45
@22:12:42.60 PCR.run 10:00
@22:15:38.83 PCR.off
@22:15:38.88 AFV.select :carrierSolution
@22:15:38.99 delay 2 seconds
@22:15:41.01 AS.seek 1ml
@22:16:38.77 delay 1 second
@22:16:39.79 AFV.select :holdingCoil
@22:16:39.90 delay 1 second
@22:16:40.92 AS.reconfigure ASfast
@22:16:40.97 AS.seek 0ml
@22:17:08.30 delay 3 seconds
@22:17:11.32 AFV.select :carrierSolution
@22:17:11.43 delay 2 seconds
@22:17:13.45 AS.reconfigure ASconfig
@22:17:13.51 AS.seek 1ml
@22:18:11.27 delay 1 second
@22:18:12.29 AFV.select :holdingCoil
@22:18:12.40 delay 1 second
@22:18:13.42 AS.reconfigure ASfast
@22:18:13.48 AS.seek 0ml
@22:18:40.82 delay 3 seconds
@22:18:43.84 AFV.select :carrierSolution
@22:18:43.95 delay 2 seconds
@22:18:45.97 AS.reconfigure ASconfig
@22:18:46.03 AS.seek 1ml
@22:19:43.82 delay 1 second
@22:19:44.84 AFV.select :holdingCoil
@22:19:44.95 delay 1 second
@22:19:45.97 AS.reconfigure ASfast
@22:19:46.02 AS.seek 0ml
@22:20:13.33 delay 3 seconds
@22:20:16.35 AFV.select :carrierSolution
@22:20:16.46 delay 2 seconds
@22:20:18.48 AS.reconfigure ASconfig
@22:20:18.54 AS.seek 1ml
@22:21:16.29 delay 1 second
@22:21:17.31 AFV.select :holdingCoil
@22:21:17.42 delay 1 second
@22:21:18.44 AS.reconfigure ASfast
@22:21:18.50 AS.seek 0ml
@22:21:45.83 delay 3 seconds
@22:21:48.85 AFV.select :carrierSolution
@22:21:48.96 delay 2 seconds
@22:21:50.98 AS.reconfigure ASconfig
@22:21:51.04 AS.seek 1ml
@22:22:48.80 delay 1 second
@22:22:49.82 AFV.select :holdingCoil
@22:22:49.93 delay 1 second
@22:22:50.95 AS.reconfigure ASnorm
@22:22:51.01 AS.seek 0ml
@22:23:41.75 Setting up PCR for ACOPP5 -- writing /var/log/esp/ACOPP521apr0922h.pcr
@22:23:41.76 Building PCR Slug from ASV with MM3 mix, PP5 primer
@22:23:41.79 Priming with carrier
@22:23:41.80 ATV.dial ARV
@22:23:42.46 ARV.dial :carrier
@22:23:42.82 AS.seek 0.5ml
@22:24:08.35 AFV.select :carrierSolution
@22:24:08.48 delay 2 seconds
@22:24:10.50 AS.reconfigure ASconfig
@22:24:10.56 AS.seek 1ml
@22:24:39.57 delay 1 second
@22:24:40.59 AFV.select :holdingCoil
@22:24:40.70 delay 1 second
@22:24:41.72 ATV.dial :waste
@22:24:43.50 AS.reconfigure ASnorm
@22:24:43.56 AS.seek 0ml
@22:25:34.26 AFV.select :carrierSolution
@22:25:34.38 delay 2 seconds
@22:25:36.40 AS.reconfigure ASconfig
@22:25:36.46 AS.seek 0.5ml
@22:26:05.52 delay 1 second
@22:26:06.55 AFV.select :holdingCoil
@22:26:06.65 Building Slug
@22:26:06.66 ATV.dial :ARV
@22:26:08.41 delay 2 seconds
@22:26:10.44 ARV.dial :air1
@22:26:10.84 delay 2 seconds
@22:26:12.91 AS.reconfigure ASslow2
@22:26:12.97 AS.seek 0.506ml
@22:26:19.18 delay 2 seconds
@22:26:21.21 ARV.dial :MM3
@22:26:22.30 delay 2 seconds
@22:26:24.33 Adding MM3
@22:26:24.41 AS.seek 0.524ml
@22:26:42.86 delay 25 seconds
@22:27:07.89 ARV.dial :PP5
@22:27:08.30 Adding PP5
@22:27:08.32 delay 2 seconds
@22:27:10.39 AS.seek 0.53ml
@22:27:16.63 delay 10 seconds
@22:27:26.67 ARV.dial :air3
@22:27:27.08 adding ARV air
@22:27:27.10 delay 2 seconds
@22:27:29.17 AS.seek 0.536ml
@22:27:35.35 delay 5 seconds
@22:27:40.38 ARV.dial :carrier
@22:27:41.72 delay 7 seconds
@22:27:48.79 AS.seek 0.594ml
@22:28:47.52 delay 5 seconds
@22:28:52.55 ATV.dial :ASV
@22:28:52.97 delay 2 seconds
@22:28:55.04 AS.seek 0.6ml
@22:29:01.93 delay 4 seconds
@22:29:05.96 ATV.dial ARV
@22:29:06.55 delay 2 seconds
@22:29:08.58 ARV.dial :carrier
@22:29:08.59 delay 2 seconds
@22:29:10.67 AS.seek 0.65ml
@22:30:01.68 delay 4 seconds
@22:30:05.71 ATV.dial PCR
@22:30:06.35 ASV.dial :waste1
@22:30:06.37 ARV.dial :air1
@22:30:06.81 Positioning Slug by 0.324ml
@22:30:06.89 AS.seek 0.326ml
@22:35:33.10 delay 5 seconds
@22:35:38.13 ATV.dial ARV
@22:35:38.94 ASV.dial :PCRlock
@22:35:39.35 delay 5 seconds
@22:35:44.38 Running ACOPP5 ProfileIPc thermocycling
@22:35:44.39 <PCR> PCR.program ProfileIPc
@22:35:44.51 ProfileIPc should complete in < 1:26:35
@22:35:44.52 PCR.run 1:53:13.75
@23:44:35.44 PCR.off
@23:44:36.01 <MFBPCR> PCR for ACOPP5 completed -- closed /var/log/esp/ACOPP521apr0922h.pcr
@23:44:36.09 Cleaning PCR
@23:44:36.10 AFV.select :carrierSolution
@23:44:36.22 delay 2 seconds
@23:44:38.24 AS.reconfigure ASconfig
@23:44:38.30 AS.seek 0.5ml
@23:44:48.63 delay 1 second
@23:44:49.65 AFV.select :holdingCoil
@23:44:49.76 ASV.dial :waste1
@23:44:50.15 ATV.dial :air3
@23:44:51.49 AS.reconfigure ASslow1
@23:44:51.55 AS.seek 0.51ml
@23:44:53.92 delay 2 seconds
@23:44:55.95 ATV.dial :bleach
@23:44:56.58 AS.seek 0.81ml
@23:45:57.19 delay 3 seconds
@23:46:00.22 ATV.dial :air3
@23:46:00.80 delay 3 seconds
@23:46:03.87 AS.seek 0.835ml
@23:46:09.22 delay 3 seconds
@23:46:12.25 ATV.dial :bleach
@23:46:12.89 AS.seek 0.82ml
@23:46:16.19 delay 3 seconds
@23:46:19.22 ARV.dial :waste
@23:46:19.87 delay 3 seconds
@23:46:22.90 ATV.dial :ARV
@23:46:23.85 AS.reconfigure ASconfig
@23:46:23.90 AS.seek 0.47ml
@23:46:44.32 AFV.select :carrierSolution
@23:46:44.44 delay 2 seconds
@23:46:46.46 AS.seek 1ml
@23:47:17.21 delay 1 second
@23:47:18.23 AFV.select :holdingCoil
@23:47:18.34 delay 1 second
@23:47:19.36 AS.reconfigure ASfast
@23:47:19.41 AS.seek 0ml
@23:47:46.74 AFV.select :carrierSolution
@23:47:46.86 delay 2 seconds
@23:47:48.88 AS.reconfigure ASconfig
@23:47:48.93 AS.seek 1ml
@23:48:46.68 delay 1 second
@23:48:47.70 AFV.select :holdingCoil
@23:48:47.81 delay 1 second
@23:48:48.83 AS.reconfigure ASfast
@23:48:48.88 AS.seek 0ml
@23:49:16.22 AFV.select :carrierSolution
@23:49:16.34 delay 2 seconds
@23:49:18.36 AS.reconfigure ASconfig
@23:49:18.41 AS.seek 1ml
@23:50:16.16 delay 1 second
@23:50:17.18 AFV.select :holdingCoil
@23:50:17.29 delay 1 second
@23:50:18.31 AS.reconfigure ASfast
@23:50:18.36 AS.seek 0ml
@23:50:45.71 ARV.dial between carrier and air1
@23:50:46.25 ATV.dial :air3
@23:50:47.60 AS.reconfigure ASslow1
@23:50:47.66 AS.seek 0.01ml
@23:50:49.96 delay 2 seconds
@23:50:51.99 ATV.dial :bleach
@23:50:52.63 AS.seek 0.81ml
@23:53:33.91 delay 3 seconds
@23:53:36.94 ATV.dial :air3
@23:53:37.52 delay 3 seconds
@23:53:40.59 AS.seek 0.835ml
@23:53:45.89 delay 3 seconds
@23:53:48.92 ATV.dial :bleach
@23:53:49.57 AS.seek 0.82ml
@23:53:52.88 delay 3 seconds
@23:53:55.91 ATV.dial PCR
@23:53:56.34 AS.reconfigure ASconfig
@23:53:56.39 AS.seek 0ml
@23:54:43.80 PCR.program :bleachProfile
@23:54:43.87 PCR program should complete in < 2:45
@23:54:43.88 PCR.run 10:00
@23:57:40.09 PCR.off
@23:57:40.12 AFV.select :carrierSolution
@23:57:40.24 delay 2 seconds
@23:57:42.26 AS.seek 1ml
@23:58:40.01 delay 1 second
@23:58:41.03 AFV.select :holdingCoil
@23:58:41.14 delay 1 second
@23:58:42.16 AS.reconfigure ASfast
@23:58:42.21 AS.seek 0ml
@23:59:09.54 delay 3 seconds
@23:59:12.56 AFV.select :carrierSolution
@23:59:12.67 delay 2 seconds
@23:59:14.69 AS.reconfigure ASconfig
@23:59:14.76 AS.seek 1ml
@00:00:12.54CEST10-Apr-21 delay 1 second
@00:00:13.56 AFV.select :holdingCoil
@00:00:13.67 delay 1 second
@00:00:14.69 AS.reconfigure ASfast
@00:00:14.75 AS.seek 0ml
@00:00:42.10 delay 3 seconds
@00:00:45.12 AFV.select :carrierSolution
@00:00:45.23 delay 2 seconds
@00:00:47.25 AS.reconfigure ASconfig
@00:00:47.32 AS.seek 1ml
@00:01:45.05 delay 1 second
@00:01:46.07 AFV.select :holdingCoil
@00:01:46.18 delay 1 second
@00:01:47.20 AS.reconfigure ASfast
@00:01:47.26 AS.seek 0ml
@00:02:14.60 delay 3 seconds
@00:02:17.62 AFV.select :carrierSolution
@00:02:17.73 delay 2 seconds
@00:02:19.75 AS.reconfigure ASconfig
@00:02:19.82 AS.seek 1ml
@00:03:17.57 delay 1 second
@00:03:18.59 AFV.select :holdingCoil
@00:03:18.70 delay 1 second
@00:03:19.72 AS.reconfigure ASfast
@00:03:19.77 AS.seek 0ml
@00:03:47.12 delay 3 seconds
@00:03:50.14 AFV.select :carrierSolution
@00:03:50.25 delay 2 seconds
@00:03:52.27 AS.reconfigure ASconfig
@00:03:52.33 AS.seek 1ml
@00:04:50.08 delay 1 second
@00:04:51.10 AFV.select :holdingCoil
@00:04:51.21 delay 1 second
@00:04:52.23 AS.reconfigure ASnorm
@00:04:52.28 AS.seek 0ml
@00:05:42.99 Setting up PCR for AIPc -- writing /var/log/esp/AIPc21apr1000h.pcr
@00:05:43.00 Building PCR Slug from ASV with MM3 mix, PP6 primer
@00:05:43.03 Priming with carrier
@00:05:43.04 ATV.dial ARV
@00:05:43.69 ARV.dial :carrier
@00:05:44.04 AS.seek 0.5ml
@00:06:09.65 AFV.select :carrierSolution
@00:06:09.78 delay 2 seconds
@00:06:11.80 AS.reconfigure ASconfig
@00:06:11.86 AS.seek 1ml
@00:06:40.88 delay 1 second
@00:06:41.90 AFV.select :holdingCoil
@00:06:42.01 delay 1 second
@00:06:43.03 ATV.dial :waste
@00:06:44.80 AS.reconfigure ASnorm
@00:06:44.86 AS.seek 0ml
@00:07:35.52 AFV.select :carrierSolution
@00:07:35.64 delay 2 seconds
@00:07:37.66 AS.reconfigure ASconfig
@00:07:37.71 AS.seek 0.5ml
@00:08:06.77 delay 1 second
@00:08:07.79 AFV.select :holdingCoil
@00:08:07.90 Building Slug
@00:08:07.91 ATV.dial :ARV
@00:08:09.66 delay 2 seconds
@00:08:11.68 ARV.dial :air1
@00:08:12.07 delay 2 seconds
@00:08:14.14 AS.reconfigure ASslow2
@00:08:14.20 AS.seek 0.506ml
@00:08:20.69 delay 2 seconds
@00:08:22.72 ARV.dial :MM3
@00:08:23.82 delay 2 seconds
@00:08:25.85 Adding MM3
@00:08:25.93 AS.seek 0.524ml
@00:08:44.24 delay 25 seconds
@00:09:09.27 ARV.dial :PP6
@00:09:09.66 Adding PP6
@00:09:09.68 delay 2 seconds
@00:09:11.75 AS.seek 0.53ml
@00:09:18.04 delay 10 seconds
@00:09:28.48 ARV.dial :air1
@00:09:29.36 adding ARV air
@00:09:29.37 delay 2 seconds
@00:09:31.44 AS.seek 0.536ml
@00:09:37.68 delay 5 seconds
@00:09:42.71 ARV.dial :carrier
@00:09:43.12 delay 7 seconds
@00:09:50.20 AS.seek 0.594ml
@00:10:48.99 delay 5 seconds
@00:10:54.02 ATV.dial :ASV
@00:10:54.44 delay 2 seconds
@00:10:56.52 AS.seek 0.6ml
@00:11:02.77 delay 4 seconds
@00:11:06.80 ATV.dial ARV
@00:11:07.38 delay 2 seconds
@00:11:09.41 ARV.dial :carrier
@00:11:09.42 delay 2 seconds
@00:11:11.49 AS.seek 0.65ml
@00:12:02.01 delay 4 seconds
@00:12:06.04 ATV.dial PCR
@00:12:06.68 ASV.dial :waste1
@00:12:06.70 ARV.dial :air1
@00:12:07.11 Positioning Slug by 0.324ml
@00:12:07.19 AS.seek 0.326ml
@00:17:33.21 delay 5 seconds
@00:17:38.24 ATV.dial ARV
@00:17:39.05 ASV.dial :PCRlock
@00:17:39.46 delay 5 seconds
@00:17:44.49 Running AIPc ProfileIPc thermocycling
@00:17:44.50 <PCR> PCR.program ProfileIPc
@00:17:44.62 ProfileIPc should complete in < 1:26:35
@00:17:44.63 PCR.run 1:53:13.75
@01:26:23.79 PCR.off
@01:26:24.36 <MFBPCR> PCR for AIPc completed -- closed /var/log/esp/AIPc21apr1000h.pcr
@01:26:24.38 Full MFB Decontamination
@01:26:24.39 Decon extraction column and decon extSrc
@01:26:24.43 SPE.hold 2:46:40,55C
@01:26:24.47 ASV.dial :SPEdelivery
@01:26:25.16 delay 20 seconds
@01:26:45.19 AFV.select :carrierSolution
@01:26:45.30 delay 2 seconds
@01:26:47.32 AS.reconfigure ASconfig
@01:26:47.38 AS.seek 0.5ml
@01:26:57.68 delay 1 second
@01:26:58.70 AFV.select :holdingCoil
@01:26:58.81 ATV.dial :air1
@01:27:00.86 AS.reconfigure ASslow1
@01:27:00.92 AS.seek 0.52ml
@01:27:05.29 ATV.dial :ASV
@01:27:07.24 ASV.dial :SPErecovery
@01:27:07.94 AS.seek 0.62ml
@01:27:28.29 ATV.dial :waste
@01:27:30.52 AS.reconfigure ASnorm
@01:27:30.58 AS.seek 0ml
@01:28:02.13 deconEluateRecoveryLine extSrc
@01:28:02.14 ATV.dial :waste
@01:28:02.17 AS.seek 0ml
@01:28:02.26 ATV.dial :air3
@01:28:03.02 AS.reconfigure ASslow1
@01:28:03.08 AS.seek 0.01ml
@01:28:05.41 delay 2 seconds
@01:28:07.44 ATV.dial :bleach
@01:28:07.94 AS.seek 0.31ml
@01:29:08.54 delay 3 seconds
@01:29:11.57 ATV.dial :air3
@01:29:12.18 delay 3 seconds
@01:29:15.26 AS.seek 0.335ml
@01:29:20.56 delay 3 seconds
@01:29:23.59 ATV.dial :bleach
@01:29:24.25 AS.seek 0.32ml
@01:29:27.49 delay 3 seconds
@01:29:30.52 ATV.dial :extSrc
@01:29:31.20 AS.seek 0ml
@01:30:35.84 AFV.select :carrierSolution
@01:30:35.96 delay 2 seconds
@01:30:37.98 AS.reconfigure ASconfig
@01:30:38.03 AS.seek 0.5ml
@01:31:07.09 delay 1 second
@01:31:08.11 AFV.select :holdingCoil
@01:31:08.22 ATV.dial :extSrc
@01:31:08.24 AS.reconfigure ASslow1
@01:31:08.31 AS.seek 0ml
@01:32:49.26 AFV.select :carrierSolution
@01:32:49.38 delay 2 seconds
@01:32:51.40 AS.reconfigure ASconfig
@01:32:51.46 AS.seek 0.5ml
@01:33:20.49 delay 1 second
@01:33:21.51 AFV.select :holdingCoil
@01:33:21.62 ATV.dial :extSrc
@01:33:21.64 AS.reconfigure ASslow1
@01:33:21.71 AS.seek 0ml
@01:35:02.52 AFV.select :carrierSolution
@01:35:02.64 delay 2 seconds
@01:35:04.66 AS.reconfigure ASconfig
@01:35:04.72 AS.seek 0.5ml
@01:35:33.76 delay 1 second
@01:35:34.78 AFV.select :holdingCoil
@01:35:34.89 ATV.dial :extSrc
@01:35:34.91 AS.reconfigure ASslow1
@01:35:34.98 AS.seek 0ml
@01:37:15.82 AFV.select :carrierSolution
@01:37:15.94 delay 2 seconds
@01:37:17.96 AS.reconfigure ASconfig
@01:37:18.01 AS.seek 0.5ml
@01:37:47.05 delay 1 second
@01:37:48.07 AFV.select :holdingCoil
@01:37:48.18 ATV.dial :extSrc
@01:37:48.20 AS.reconfigure ASslow1
@01:37:48.27 AS.seek 0ml
@01:39:29.09 ATV.dial :air2
@01:39:30.49 AS.reconfigure ASslow0
@01:39:30.55 AS.seek 0.5ml
@01:40:21.16 ATV.dial :extSrc
@01:40:22.72 AS.seek 0.02ml
@01:41:11.32 delay 1 second
@01:41:12.76 ATV.dial :waste
@01:41:14.11 AS.reconfigure ASconfig
@01:41:14.16 AS.seek 0ml
@01:41:15.60 AFV.select :holdingCoil
@01:41:15.62 ATV.dial :waste
@01:41:15.65 AS.seek 0ml
@01:41:15.73 AFV.select :carrierSolution
@01:41:15.84 delay 2 seconds
@01:41:17.86 AS.seek 0.5ml
@01:41:46.88 delay 1 second
@01:41:47.90 AFV.select :holdingCoil
@01:41:48.01 ATV.dial :air3
@01:41:48.88 AS.reconfigure ASslow1
@01:41:48.94 AS.seek 0.52ml
@01:41:53.23 ATV.dial :air3
@01:41:53.32 AS.seek 0.53ml
@01:41:55.52 delay 2 seconds
@01:41:57.55 ATV.dial :bleach
@01:41:58.04 AS.seek 0.71ml
@01:42:34.52 delay 3 seconds
@01:42:37.55 ATV.dial :air3
@01:42:38.15 delay 3 seconds
@01:42:41.23 AS.seek 0.735ml
@01:42:46.53 delay 3 seconds
@01:42:49.56 ATV.dial :bleach
@01:42:50.21 AS.seek 0.72ml
@01:42:53.65 delay 3 seconds
@01:42:56.68 ATV.dial ASV
@01:42:57.77 ASV.dial :SPErecovery
@01:42:57.86 AS.seek 0.365ml
@01:44:09.56 ATV.dial :waste
@01:44:11.41 AS.reconfigure ASnorm
@01:44:11.47 AS.seek 0ml
@01:44:30.18 ATV.dial :air3
@01:44:30.91 AS.reconfigure ASslow1
@01:44:30.97 AS.seek 0.01ml
@01:44:33.30 delay 2 seconds
@01:44:35.33 ATV.dial :bleach
@01:44:35.80 AS.seek 0.58ml
@01:46:30.68 delay 3 seconds
@01:46:33.71 ATV.dial :air3
@01:46:34.30 delay 3 seconds
@01:46:37.37 AS.seek 0.605ml
@01:46:42.74 delay 3 seconds
@01:46:45.77 ATV.dial :bleach
@01:46:46.41 AS.seek 0.59ml
@01:46:49.68 delay 3 seconds
@01:46:52.71 ATV.dial :ASV
@01:46:53.80 ASV.dial :wasteR
@01:46:54.21 AS.seek 0ml
@01:48:53.10 delay 1:00
@01:49:53.14 ATV.dial ASV
@01:49:53.15 AFV.select :carrierSolution
@01:49:53.27 delay 2 seconds
@01:49:55.29 AS.reconfigure ASconfig
@01:49:55.34 AS.seek 1ml
@01:50:53.10 delay 1 second
@01:50:54.12 AFV.select :holdingCoil
@01:50:54.23 delay 1 second
@01:50:55.25 ASV.dial :wasteR
@01:50:55.26 AS.reconfigure ASslow1
@01:50:55.34 AS.seek 0ml
@01:54:16.71 delay 10 seconds
@01:54:26.75 ATV.dial :air3
@01:54:28.35 AS.seek 0.01ml
@01:54:30.68 delay 2 seconds
@01:54:32.71 ATV.dial :bleach
@01:54:33.36 AS.seek 0.58ml
@01:56:28.23 delay 3 seconds
@01:56:31.26 ATV.dial :air3
@01:56:31.84 delay 3 seconds
@01:56:34.92 AS.seek 0.605ml
@01:56:40.21 delay 3 seconds
@01:56:43.24 ATV.dial :bleach
@01:56:43.88 AS.seek 0.59ml
@01:56:47.19 delay 3 seconds
@01:56:50.22 ATV.dial ASV
@01:56:51.32 ASV.dial :SPEdelivery
@01:56:51.72 AS.seek 0ml
@01:58:50.63 delay 1:00
@01:59:50.66 AFV.select :carrierSolution
@01:59:50.77 delay 2 seconds
@01:59:52.79 AS.reconfigure ASconfig
@01:59:52.85 AS.seek 1ml
@02:00:50.59 delay 1 second
@02:00:51.61 AFV.select :holdingCoil
@02:00:51.72 delay 1 second
@02:00:52.74 ATV.dial ASV
@02:00:52.75 ASV.dial :SPEdelivery
@02:00:52.77 AS.reconfigure ASslow1
@02:00:52.84 AS.seek 0ml
@02:04:14.24 delay 10 seconds
@02:04:24.28 ATV.dial :air2
@02:04:25.16 AS.reconfigure ASslow0
@02:04:25.21 AS.seek 0.8ml
@02:05:46.01 delay 3 seconds
@02:05:49.04 ATV.dial :ASV
@02:05:50.09 ASV.dial :SPEdelivery
@02:05:50.11 AS.reconfigure ASslow1
@02:05:50.18 AS.seek 0.03ml
@02:08:25.32 delay 20 seconds
@02:08:45.36 ATV.dial :waste,via: :air3
@02:08:47.19 AS.reconfigure ASconfig
@02:08:47.24 AS.seek 0ml
@02:08:49.27 SPE.coast
@02:08:49.36 Removing Bleach from Mixing Coil
@02:08:49.37 AFV.select :carrierSolution
@02:08:49.48 delay 2 seconds
@02:08:51.50 AS.seek 0.5ml
@02:09:20.55 delay 1 second
@02:09:21.93 AFV.select :holdingCoil
@02:09:22.12 ATV.dial :ASV
@02:09:23.91 ASV.dial :SPErecovery
@02:09:24.57 AS.reconfigure ASslow1
@02:09:24.63 AS.seek 0.73ml
@02:10:11.23 ASV.dial :wasteD
@02:10:11.85 AS.reconfigure ASnorm
@02:10:11.91 AS.seek 0ml
@02:10:48.98 AFV.select :carrierSolution
@02:10:49.10 delay 2 seconds
@02:10:51.12 AS.reconfigure ASconfig
@02:10:51.18 AS.seek 0.5ml
@02:11:20.23 delay 1 second
@02:11:21.25 AFV.select :holdingCoil
@02:11:21.36 ATV.dial ASV
@02:11:21.37 ASV.dial :SPErecovery
@02:11:22.04 AS.reconfigure ASslow0
@02:11:22.10 AS.seek 0.3ml
@02:11:42.52 ATV.dial :air2
@02:11:43.61 AS.reconfigure ASslow1
@02:11:43.67 AS.seek 0.32ml
@02:11:48.09 ATV.dial ASV
@02:11:49.19 AS.reconfigure ASslow0
@02:11:49.25 AS.seek 0.57ml
@02:12:14.74 ASV.dial :wasteD
@02:12:15.35 AS.reconfigure ASnorm
@02:12:15.41 AS.seek 0ml
@02:12:44.48 AFV.select :carrierSolution
@02:12:44.60 delay 2 seconds
@02:12:46.62 AS.reconfigure ASconfig
@02:12:46.67 AS.seek 0.5ml
@02:13:15.71 delay 1 second
@02:13:16.73 AFV.select :holdingCoil
@02:13:16.84 ATV.dial ASV
@02:13:16.85 ASV.dial :SPErecovery
@02:13:17.52 AS.reconfigure ASslow0
@02:13:17.58 AS.seek 0.3ml
@02:13:38.04 ATV.dial :air2
@02:13:39.13 AS.reconfigure ASslow1
@02:13:39.19 AS.seek 0.32ml
@02:13:43.52 ATV.dial ASV
@02:13:44.60 AS.reconfigure ASslow0
@02:13:44.66 AS.seek 0.57ml
@02:14:10.13 ASV.dial :wasteD
@02:14:10.74 AS.reconfigure ASnorm
@02:14:10.79 AS.seek 0ml
@02:14:39.87 ATV.dial :air2
@02:14:40.91 AS.reconfigure ASslow0
@02:14:40.97 AS.seek 0.3ml
@02:15:11.54 ATV.dial :ASV
@02:15:12.57 ASV.dial :wasteD
@02:15:12.59 AS.reconfigure ASconfig
@02:15:12.66 AS.seek 0.05ml
@02:15:27.35 ATV.dial :waste
@02:15:29.33 AS.seek 0ml
@02:15:32.50 ASV.dial :SPEdelivery
@02:15:33.60 Cleaning PCR
@02:15:33.61 AFV.select :carrierSolution
@02:15:33.73 delay 2 seconds
@02:15:35.75 AS.seek 0.5ml
@02:16:04.77 delay 1 second
@02:16:05.79 AFV.select :holdingCoil
@02:16:05.90 ASV.dial :waste1
@02:16:06.27 ATV.dial :air3
@02:16:06.99 AS.reconfigure ASslow1
@02:16:07.05 AS.seek 0.51ml
@02:16:09.38 delay 2 seconds
@02:16:11.41 ATV.dial :bleach
@02:16:11.88 AS.seek 0.81ml
@02:17:12.48 delay 3 seconds
@02:17:15.51 ATV.dial :air3
@02:17:16.09 delay 3 seconds
@02:17:19.16 AS.seek 0.835ml
@02:17:24.47 delay 3 seconds
@02:17:27.50 ATV.dial :bleach
@02:17:28.15 AS.seek 0.82ml
@02:17:31.54 delay 3 seconds
@02:17:34.57 ARV.dial :waste
@02:17:35.20 delay 3 seconds
@02:17:38.23 ATV.dial :ARV
@02:17:39.15 AS.reconfigure ASconfig
@02:17:39.21 AS.seek 0.47ml
@02:17:59.65 AFV.select :carrierSolution
@02:17:59.77 delay 2 seconds
@02:18:01.79 AS.seek 1ml
@02:18:32.57 delay 1 second
@02:18:33.59 AFV.select :holdingCoil
@02:18:33.70 delay 1 second
@02:18:34.72 AS.reconfigure ASfast
@02:18:34.78 AS.seek 0ml
@02:19:02.13 AFV.select :carrierSolution
@02:19:02.25 delay 2 seconds
@02:19:04.27 AS.reconfigure ASconfig
@02:19:04.32 AS.seek 1ml
@02:20:02.09 delay 1 second
@02:20:03.11 AFV.select :holdingCoil
@02:20:03.22 delay 1 second
@02:20:04.24 AS.reconfigure ASfast
@02:20:04.30 AS.seek 0ml
@02:20:31.63 AFV.select :carrierSolution
@02:20:31.75 delay 2 seconds
@02:20:33.77 AS.reconfigure ASconfig
@02:20:33.82 AS.seek 1ml
@02:21:31.55 delay 1 second
@02:21:32.57 AFV.select :holdingCoil
@02:21:32.68 delay 1 second
@02:21:33.70 AS.reconfigure ASfast
@02:21:33.75 AS.seek 0ml
@02:22:01.09 ARV.dial between carrier and air1
@02:22:02.06 ATV.dial :air3
@02:22:03.40 AS.reconfigure ASslow1
@02:22:03.46 AS.seek 0.01ml
@02:22:05.79 delay 2 seconds
@02:22:07.82 ATV.dial :bleach
@02:22:08.46 AS.seek 0.81ml
@02:24:49.59 delay 3 seconds
@02:24:52.62 ATV.dial :air3
@02:24:53.20 delay 3 seconds
@02:24:56.28 AS.seek 0.835ml
@02:25:01.66 delay 3 seconds
@02:25:04.69 ATV.dial :bleach
@02:25:05.33 AS.seek 0.82ml
@02:25:08.60 delay 3 seconds
@02:25:11.63 ATV.dial PCR
@02:25:12.05 AS.reconfigure ASconfig
@02:25:12.10 AS.seek 0ml
@02:25:59.52 PCR.program :bleachProfile
@02:25:59.59 PCR program should complete in < 2:45
@02:25:59.61 PCR.run 10:00
@02:29:01.49 PCR.off
@02:29:01.53 AFV.select :carrierSolution
@02:29:01.64 delay 2 seconds
@02:29:03.66 AS.seek 1ml
@02:30:01.45 delay 1 second
@02:30:02.47 AFV.select :holdingCoil
@02:30:02.58 delay 1 second
@02:30:03.60 AS.reconfigure ASfast
@02:30:03.65 AS.seek 0ml
@02:30:30.99 delay 3 seconds
@02:30:34.01 AFV.select :carrierSolution
@02:30:34.12 delay 2 seconds
@02:30:36.14 AS.reconfigure ASconfig
@02:30:36.20 AS.seek 1ml
@02:31:33.95 delay 1 second
@02:31:34.97 AFV.select :holdingCoil
@02:31:35.08 delay 1 second
@02:31:36.10 AS.reconfigure ASfast
@02:31:36.16 AS.seek 0ml
@02:32:03.48 delay 3 seconds
@02:32:06.50 AFV.select :carrierSolution
@02:32:06.61 delay 2 seconds
@02:32:08.63 AS.reconfigure ASconfig
@02:32:08.70 AS.seek 1ml
@02:33:06.45 delay 1 second
@02:33:07.47 AFV.select :holdingCoil
@02:33:07.58 delay 1 second
@02:33:08.60 AS.reconfigure ASfast
@02:33:08.65 AS.seek 0ml
@02:33:35.98 delay 3 seconds
@02:33:39.00 AFV.select :carrierSolution
@02:33:39.11 delay 2 seconds
@02:33:41.13 AS.reconfigure ASconfig
@02:33:41.19 AS.seek 1ml
@02:34:38.93 delay 1 second
@02:34:39.95 AFV.select :holdingCoil
@02:34:40.06 delay 1 second
@02:34:41.08 AS.reconfigure ASfast
@02:34:41.14 AS.seek 0ml
@02:35:08.48 delay 3 seconds
@02:35:11.89 AFV.select :carrierSolution
@02:35:12.02 delay 2 seconds
@02:35:14.04 AS.reconfigure ASconfig
@02:35:14.10 AS.seek 1ml
@02:36:11.84 delay 1 second
@02:36:12.86 AFV.select :holdingCoil
@02:36:12.97 delay 1 second
@02:36:13.99 AS.reconfigure ASnorm
@02:36:14.05 AS.seek 0ml
@02:37:04.79 AFV.select :carrierSolution
@02:37:04.90 delay 2 seconds
@02:37:06.92 AS.reconfigure ASconfig
@02:37:06.97 AS.seek 1ml
@02:38:04.74 delay 1 second
@02:38:05.76 AFV.select :holdingCoil
@02:38:05.87 delay 1 second
@02:38:06.89 ATV.dial :waste
@02:38:08.16 AS.reconfigure ASnorm
@02:38:08.22 AS.seek 0.5ml
@02:38:33.77 ATV.dial :waste2
@02:38:35.13 AS.seek 0ml
@02:39:00.71 <mwj> Session Ended
@09:53:07.74CEST12-Apr-21 <MAIN> ESP2 v3.30 I2C Gateway v3.06 for core bus
@09:53:10.25 
Configuration for mer 7/20/20 brent@mbari.org
/home/esp/esp2/type/shallow/mer/configure.rb
@09:53:10.34 
Configuration for MFB3 by preston@mbari.org
/home/esp/esp2/type/mfb/MFB3/configure.rb
@09:53:15.58 Polling Can every 10:00
@09:53:17.41 CS.configure :default
@09:53:17.48 CS.hold
@09:53:17.58 CS.configure :default
@09:53:17.75 PS.configure :default
@09:53:17.91 SS.configure :default
@09:53:17.99 SS.hold
@09:53:18.05 SS.configure :default
@09:53:18.13 CC.configure :default
@09:53:18.17 CC.hold
@09:53:18.23 CC.configure :default
@09:53:18.31 PC.configure :default
@09:53:18.35 PC.hold
@09:53:18.40 PC.configure :default
@09:53:18.50 Forearm.configure :loose
@09:53:18.54 Forearm.hold
@09:53:18.59 Forearm.configure :loose
@09:53:18.67 Elbow.configure :loose
@09:53:18.71 Elbow.hold
@09:53:18.76 Elbow.configure :loose
@09:53:18.86 SC.configure :loose
@09:53:18.90 SC.hold
@09:53:18.95 SC.configure :loose
@09:53:19.03 SE.configure :default
@09:53:19.06 SE.hold
@09:53:19.12 SE.configure :default
@09:53:19.16 CH.coast
@09:53:19.21 CH.configure :default
@09:53:19.23 PH.coast
@09:53:19.28 PH.configure :default
@09:53:19.38 SPE.coast
@09:53:19.43 SPE.configure SPEconfig
@09:53:19.50 AS.configure ASconfig
@09:53:19.54 AS.hold
@09:53:19.60 AS.configure ASconfig
@09:53:19.65 PCR.off
@09:53:19.68 PCR.pidtemp= 50,0.1,0,300,25,25
@09:53:19.71 PCR.caltemp= 2.5051,0.0372,0
@09:53:19.74 All dwarf controllers running firmware version 3.75
@09:53:19.94 Cleanly exited at 02:39:00CEST10-Apr-21
@09:53:19.97 Loading /home/esp/esp2/mission/GENOMAP_v2.rb mission
@09:53:20.07 Cleaning PCR
@09:53:20.09 AFV.select :carrierSolution
@09:53:20.21 delay 2 seconds
@09:53:20.61 <mwj> Session Begun
@09:53:22.24 <MAIN> AS.seek 0.5ml
@09:53:27.09 <mwj> -> exit
@09:53:27.10 Session Ended
@09:53:51.33 <MAIN> delay 1 second
@09:53:52.35 AFV.select :holdingCoil
@09:53:52.46 ASV.dial :waste1
@09:53:52.52 ATV.dial :air3
@09:53:54.63 AS.reconfigure ASslow1
@09:53:54.67 AS.seek 0.51ml
@09:53:56.94 delay 2 seconds
@09:53:58.97 ATV.dial :bleach
@09:53:59.42 AS.seek 0.81ml
@09:55:00.07 delay 3 seconds
@09:55:03.10 ATV.dial :air3
@09:55:03.66 delay 3 seconds
@09:55:06.73 AS.seek 0.835ml
@09:55:12.03 delay 3 seconds
@09:55:15.06 ATV.dial :bleach
@09:55:15.69 AS.seek 0.82ml
@09:55:18.97 delay 3 seconds
@09:55:22.00 ARV.dial :waste
@09:55:22.50 delay 3 seconds
@09:55:25.53 ATV.dial :ARV
@09:55:26.45 AS.reconfigure ASconfig
@09:55:26.49 AS.seek 0.47ml
@09:55:46.88 AFV.select :carrierSolution
@09:55:46.99 delay 2 seconds
@09:55:49.01 AS.seek 1ml
@09:56:19.74 delay 1 second
@09:56:20.76 AFV.select :holdingCoil
@09:56:20.87 delay 1 second
@09:56:21.89 AS.reconfigure ASfast
@09:56:21.93 AS.seek 0ml
@09:56:49.24 AFV.select :carrierSolution
@09:56:49.35 delay 2 seconds
@09:56:51.37 AS.reconfigure ASconfig
@09:56:51.42 AS.seek 1ml
@09:57:49.22 delay 1 second
@09:57:50.23 AFV.select :holdingCoil
@09:57:50.34 delay 1 second
@09:57:51.36 AS.reconfigure ASfast
@09:57:51.41 AS.seek 0ml
@09:58:18.74 AFV.select :carrierSolution
@09:58:18.85 delay 2 seconds
@09:58:20.87 AS.reconfigure ASconfig
@09:58:20.92 AS.seek 1ml
@09:59:19.03 delay 1 second
@09:59:20.04 AFV.select :holdingCoil
@09:59:20.15 delay 1 second
@09:59:21.17 AS.reconfigure ASfast
@09:59:21.22 AS.seek 0ml
@09:59:48.53 ARV.dial between carrier and air1
@09:59:49.06 ATV.dial :air3
@09:59:50.41 AS.reconfigure ASslow1
@09:59:50.45 AS.seek 0.01ml
@09:59:52.83 delay 2 seconds
@09:59:54.86 ATV.dial :bleach
@09:59:55.50 AS.seek 0.81ml
@10:02:36.61 delay 3 seconds
@10:02:39.64 ATV.dial :air3
@10:02:40.21 delay 3 seconds
@10:02:43.28 AS.seek 0.835ml
@10:02:48.54 delay 3 seconds
@10:02:51.57 ATV.dial :bleach
@10:02:52.19 AS.seek 0.82ml
@10:02:55.61 delay 3 seconds
@10:02:58.64 ATV.dial PCR
@10:02:59.06 AS.reconfigure ASconfig
@10:02:59.10 AS.seek 0ml
@10:03:46.47 PCR.program :bleachProfile
@10:03:46.53 PCR program should complete in < 2:45
@10:03:46.54 PCR.run 10:00
@10:06:48.39 PCR.off
@10:06:48.41 AFV.select :carrierSolution
@10:06:48.52 delay 2 seconds
@10:06:50.54 AS.seek 1ml
@10:07:48.33 delay 1 second
@10:07:49.34 AFV.select :holdingCoil
@10:07:49.45 delay 1 second
@10:07:50.47 AS.reconfigure ASfast
@10:07:50.52 AS.seek 0ml
@10:08:17.83 delay 3 seconds
@10:08:20.85 AFV.select :carrierSolution
@10:08:20.96 delay 2 seconds
@10:08:22.98 AS.reconfigure ASconfig
@10:08:23.03 AS.seek 1ml
@10:09:20.80 delay 1 second
@10:09:21.82 AFV.select :holdingCoil
@10:09:21.93 delay 1 second
@10:09:22.95 AS.reconfigure ASfast
@10:09:23.00 AS.seek 0ml
@10:09:50.33 delay 3 seconds
@10:09:53.35 AFV.select :carrierSolution
@10:09:53.46 delay 2 seconds
@10:09:55.48 AS.reconfigure ASconfig
@10:09:55.53 AS.seek 1ml
@10:10:53.27 delay 1 second
@10:10:54.28 AFV.select :holdingCoil
@10:10:54.39 delay 1 second
@10:10:55.41 AS.reconfigure ASfast
@10:10:55.46 AS.seek 0ml
@10:11:22.78 delay 3 seconds
@10:11:25.80 AFV.select :carrierSolution
@10:11:25.91 delay 2 seconds
@10:11:27.93 AS.reconfigure ASconfig
@10:11:27.98 AS.seek 1ml
@10:12:25.72 delay 1 second
@10:12:26.73 AFV.select :holdingCoil
@10:12:26.84 delay 1 second
@10:12:27.86 AS.reconfigure ASfast
@10:12:27.91 AS.seek 0ml
@10:12:55.22 delay 3 seconds
@10:12:58.24 AFV.select :carrierSolution
@10:12:58.34 delay 2 seconds
@10:13:00.36 AS.reconfigure ASconfig
@10:13:00.41 AS.seek 1ml
@10:13:58.19 delay 1 second
@10:13:59.20 AFV.select :holdingCoil
@10:13:59.30 delay 1 second
@10:14:00.32 AS.reconfigure ASnorm
@10:14:00.37 AS.seek 0ml
@10:14:51.06 delay 5 seconds
@10:14:56.14 Cleaning PCR
@10:14:56.15 AFV.select :carrierSolution
@10:14:56.25 delay 2 seconds
@10:14:58.27 AS.reconfigure ASconfig
@10:14:58.32 AS.seek 0.5ml
@10:15:27.36 delay 1 second
@10:15:28.37 AFV.select :holdingCoil
@10:15:28.48 ASV.dial :waste1
@10:15:28.49 ATV.dial :air3
@10:15:29.36 AS.reconfigure ASslow1
@10:15:29.40 AS.seek 0.51ml
@10:15:31.66 delay 2 seconds
@10:15:33.68 ATV.dial :bleach
@10:15:34.32 AS.seek 0.81ml
@10:16:34.89 delay 3 seconds
@10:16:37.92 ATV.dial :air3
@10:16:38.49 delay 3 seconds
@10:16:41.56 AS.seek 0.835ml
@10:16:46.87 delay 3 seconds
@10:16:49.90 ATV.dial :bleach
@10:16:50.53 AS.seek 0.82ml
@10:16:53.82 delay 3 seconds
@10:16:56.85 ARV.dial :waste
@10:16:57.35 delay 3 seconds
@10:17:00.38 ATV.dial :ARV
@10:17:01.30 AS.reconfigure ASconfig
@10:17:01.34 AS.seek 0.47ml
@10:17:22.11 AFV.select :carrierSolution
@10:17:22.22 delay 2 seconds
@10:17:24.24 AS.seek 1ml
@10:17:54.97 delay 1 second
@10:17:55.99 AFV.select :holdingCoil
@10:17:56.10 delay 1 second
@10:17:57.12 AS.reconfigure ASfast
@10:17:57.17 AS.seek 0ml
@10:18:24.49 AFV.select :carrierSolution
@10:18:24.60 delay 2 seconds
@10:18:26.62 AS.reconfigure ASconfig
@10:18:26.67 AS.seek 1ml
@10:19:24.41 delay 1 second
@10:19:25.43 AFV.select :holdingCoil
@10:19:25.54 delay 1 second
@10:19:26.56 AS.reconfigure ASfast
@10:19:26.61 AS.seek 0ml
@10:19:53.93 AFV.select :carrierSolution
@10:19:54.04 delay 2 seconds
@10:19:56.06 AS.reconfigure ASconfig
@10:19:56.11 AS.seek 1ml
@10:20:53.88 delay 1 second
@10:20:54.90 AFV.select :holdingCoil
@10:20:55.01 delay 1 second
@10:20:56.03 AS.reconfigure ASfast
@10:20:56.08 AS.seek 0ml
@10:21:23.39 ARV.dial between carrier and air1
@10:21:23.92 ATV.dial :air3
@10:21:25.25 AS.reconfigure ASslow1
@10:21:25.29 AS.seek 0.01ml
@10:21:27.57 delay 2 seconds
@10:21:29.60 ATV.dial :bleach
@10:21:30.24 AS.seek 0.81ml
@10:24:11.39 delay 3 seconds
@10:24:14.42 ATV.dial :air3
@10:24:14.99 delay 3 seconds
@10:24:18.06 AS.seek 0.835ml
@10:24:23.33 delay 3 seconds
@10:24:26.36 ATV.dial :bleach
@10:24:26.99 AS.seek 0.82ml
@10:24:30.32 delay 3 seconds
@10:24:33.35 ATV.dial PCR
@10:24:33.76 AS.reconfigure ASconfig
@10:24:33.80 AS.seek 0ml
@10:25:21.16 PCR.program :bleachProfile
@10:25:21.22 PCR program should complete in < 2:45
@10:25:21.23 PCR.run 10:00
@10:28:23.08 PCR.off
@10:28:23.10 AFV.select :carrierSolution
@10:28:23.21 delay 2 seconds
@10:28:25.23 AS.seek 1ml
@10:29:23.00 delay 1 second
@10:29:24.01 AFV.select :holdingCoil
@10:29:24.12 delay 1 second
@10:29:25.14 AS.reconfigure ASfast
@10:29:25.19 AS.seek 0ml
@10:29:52.50 delay 3 seconds
@10:29:55.52 AFV.select :carrierSolution
@10:29:55.63 delay 2 seconds
@10:29:57.65 AS.reconfigure ASconfig
@10:29:57.70 AS.seek 1ml
@10:30:55.49 delay 1 second
@10:30:56.51 AFV.select :holdingCoil
@10:30:56.62 delay 1 second
@10:30:57.64 AS.reconfigure ASfast
@10:30:57.69 AS.seek 0ml
@10:31:25.02 delay 3 seconds
@10:31:28.04 AFV.select :carrierSolution
@10:31:28.15 delay 2 seconds
@10:31:30.17 AS.reconfigure ASconfig
@10:31:30.22 AS.seek 1ml
@10:32:27.98 delay 1 second
@10:32:29.01 AFV.select :holdingCoil
@10:32:29.14 delay 1 second
@10:32:30.18 AS.reconfigure ASfast
@10:32:30.26 AS.seek 0ml
@10:32:57.58 delay 3 seconds
@10:33:00.61 AFV.select :carrierSolution
@10:33:00.71 delay 2 seconds
@10:33:02.73 AS.reconfigure ASconfig
@10:33:02.78 AS.seek 1ml
@10:34:00.52 delay 1 second
@10:34:01.54 AFV.select :holdingCoil
@10:34:01.65 delay 1 second
@10:34:02.67 AS.reconfigure ASfast
@10:34:02.72 AS.seek 0ml
@10:34:30.04 delay 3 seconds
@10:34:33.06 AFV.select :carrierSolution
@10:34:33.16 delay 2 seconds
@10:34:35.18 AS.reconfigure ASconfig
@10:34:35.23 AS.seek 1ml
@10:35:33.00 delay 1 second
@10:35:34.01 AFV.select :holdingCoil
@10:35:34.11 delay 1 second
@10:35:35.13 AS.reconfigure ASnorm
@10:35:35.18 AS.seek 0ml
@10:36:25.88 delay 5 seconds
@10:36:30.96 Cleaning PCR
@10:36:30.97 AFV.select :carrierSolution
@10:36:31.07 delay 2 seconds
@10:36:33.09 AS.reconfigure ASconfig
@10:36:33.14 AS.seek 0.5ml
@10:37:02.18 delay 1 second
@10:37:03.20 AFV.select :holdingCoil
@10:37:03.31 ASV.dial :waste1
@10:37:03.32 ATV.dial :air3
@10:37:04.21 AS.reconfigure ASslow1
@10:37:04.25 AS.seek 0.51ml
@10:37:06.62 delay 2 seconds
@10:37:08.64 ATV.dial :bleach
@10:37:09.65 AS.seek 0.81ml
@10:38:10.24 delay 3 seconds
@10:38:13.27 ATV.dial :air3
@10:38:13.83 delay 3 seconds
@10:38:16.90 AS.seek 0.835ml
@10:38:22.19 delay 3 seconds
@10:38:25.22 ATV.dial :bleach
@10:38:25.85 AS.seek 0.82ml
@10:38:29.14 delay 3 seconds
@10:38:32.17 ARV.dial :waste
@10:38:32.68 delay 3 seconds
@10:38:35.71 ATV.dial :ARV
@10:38:36.63 AS.reconfigure ASconfig
@10:38:36.67 AS.seek 0.47ml
@10:38:57.05 AFV.select :carrierSolution
@10:38:57.16 delay 2 seconds
@10:38:59.18 AS.seek 1ml
@10:39:29.91 delay 1 second
@10:39:30.93 AFV.select :holdingCoil
@10:39:31.04 delay 1 second
@10:39:32.06 AS.reconfigure ASfast
@10:39:32.11 AS.seek 0ml
@10:39:59.44 AFV.select :carrierSolution
@10:39:59.55 delay 2 seconds
@10:40:01.57 AS.reconfigure ASconfig
@10:40:01.62 AS.seek 1ml
@10:40:59.35 delay 1 second
@10:41:00.36 AFV.select :holdingCoil
@10:41:00.47 delay 1 second
@10:41:01.49 AS.reconfigure ASfast
@10:41:01.54 AS.seek 0ml
@10:41:28.86 AFV.select :carrierSolution
@10:41:28.97 delay 2 seconds
@10:41:30.99 AS.reconfigure ASconfig
@10:41:31.04 AS.seek 1ml
@10:42:28.83 delay 1 second
@10:42:29.84 AFV.select :holdingCoil
@10:42:29.95 delay 1 second
@10:42:30.97 AS.reconfigure ASfast
@10:42:31.02 AS.seek 0ml
@10:42:58.33 ARV.dial between carrier and air1
@10:42:58.86 ATV.dial :air3
@10:43:00.28 AS.reconfigure ASslow1
@10:43:00.36 AS.seek 0.01ml
@10:43:02.80 delay 2 seconds
@10:43:04.83 ATV.dial :bleach
@10:43:05.54 AS.seek 0.81ml
@10:45:46.72 delay 3 seconds
@10:45:49.75 ATV.dial :air3
@10:45:50.32 delay 3 seconds
@10:45:53.39 AS.seek 0.835ml
@10:45:58.69 delay 3 seconds
@10:46:01.72 ATV.dial :bleach
@10:46:02.35 AS.seek 0.82ml
@10:46:05.64 delay 3 seconds
@10:46:08.67 ATV.dial PCR
@10:46:09.09 AS.reconfigure ASconfig
@10:46:09.13 AS.seek 0ml
@10:46:56.52 PCR.program :bleachProfile
@10:46:56.59 PCR program should complete in < 2:45
@10:46:56.60 PCR.run 10:00
@10:49:58.43 PCR.off
@10:49:58.45 AFV.select :carrierSolution
@10:49:58.56 delay 2 seconds
@10:50:00.58 AS.seek 1ml
@10:50:58.35 delay 1 second
@10:50:59.36 AFV.select :holdingCoil
@10:50:59.47 delay 1 second
@10:51:00.49 AS.reconfigure ASfast
@10:51:00.54 AS.seek 0ml
@10:51:27.86 delay 3 seconds
@10:51:30.88 AFV.select :carrierSolution
@10:51:30.99 delay 2 seconds
@10:51:33.01 AS.reconfigure ASconfig
@10:51:33.06 AS.seek 1ml
@10:52:30.80 delay 1 second
@10:52:31.81 AFV.select :holdingCoil
@10:52:31.92 delay 1 second
@10:52:32.94 AS.reconfigure ASfast
@10:52:32.99 AS.seek 0ml
@10:53:00.32 delay 3 seconds
@10:53:03.34 AFV.select :carrierSolution
@10:53:03.45 delay 2 seconds
@10:53:05.47 AS.reconfigure ASconfig
@10:53:05.52 AS.seek 1ml
@10:54:03.29 delay 1 second
@10:54:04.30 AFV.select :holdingCoil
@10:54:04.41 delay 1 second
@10:54:05.43 AS.reconfigure ASfast
@10:54:05.48 AS.seek 0ml
@10:54:32.82 delay 3 seconds
@10:54:35.84 AFV.select :carrierSolution
@10:54:35.95 delay 2 seconds
@10:54:37.97 AS.reconfigure ASconfig
@10:54:38.02 AS.seek 1ml
@10:55:35.75 delay 1 second
@10:55:36.76 AFV.select :holdingCoil
@10:55:36.87 delay 1 second
@10:55:37.89 AS.reconfigure ASfast
@10:55:37.94 AS.seek 0ml
@10:56:05.25 delay 3 seconds
@10:56:08.27 AFV.select :carrierSolution
@10:56:08.38 delay 2 seconds
@10:56:10.40 AS.reconfigure ASconfig
@10:56:10.45 AS.seek 1ml
@10:57:08.18 delay 1 second
@10:57:09.20 AFV.select :holdingCoil
@10:57:09.30 delay 1 second
@10:57:10.32 AS.reconfigure ASnorm
@10:57:10.73 AS.seek 0ml
@10:58:01.43 delay 5 seconds
@13:35:19.15CEST12-Apr-21 <MAIN> ESP2 v3.30 I2C Gateway v3.06 for core bus
@13:35:21.23 
Configuration for mer 7/20/20 brent@mbari.org
/home/esp/esp2/type/shallow/mer/configure.rb
@13:35:21.32 
Configuration for MFB3 by preston@mbari.org
/home/esp/esp2/type/mfb/MFB3/configure.rb
@13:35:25.69 Polling Can every 10:00
@13:35:27.41 CS.configure :default
@13:35:27.45 CS.hold
@13:35:27.52 CS.configure :default
@13:35:27.60 PS.configure :default
@13:35:27.69 SS.configure :default
@13:35:27.72 SS.hold
@13:35:27.76 SS.configure :default
@13:35:27.85 CC.configure :default
@13:35:27.88 CC.hold
@13:35:27.93 CC.configure :default
@13:35:28.01 PC.configure :default
@13:35:28.05 PC.hold
@13:35:28.10 PC.configure :default
@13:35:28.20 Forearm.configure :loose
@13:35:28.24 Forearm.hold
@13:35:28.29 Forearm.configure :loose
@13:35:28.37 Elbow.configure :loose
@13:35:28.41 Elbow.hold
@13:35:28.46 Elbow.configure :loose
@13:35:28.56 SC.configure :loose
@13:35:28.60 SC.hold
@13:35:28.64 SC.configure :loose
@13:35:28.72 SE.configure :default
@13:35:28.76 SE.hold
@13:35:28.82 SE.configure :default
@13:35:28.86 CH.coast
@13:35:28.90 CH.configure :default
@13:35:28.93 PH.coast
@13:35:28.98 PH.configure :default
@13:35:29.07 SPE.coast
@13:35:29.12 SPE.configure SPEconfig
@13:35:29.19 AS.configure ASconfig
@13:35:29.23 AS.hold
@13:35:29.29 AS.configure ASconfig
@13:35:29.34 PCR.off
@13:35:29.37 PCR.pidtemp= 50,0.1,0,300,25,25
@13:35:29.40 PCR.caltemp= 2.5051,0.0372,0
@13:35:29.43 All dwarf controllers running firmware version 3.75
@13:35:29.63 Cleanly exited at 13:34:46CEST12-Apr-21
@13:35:29.66 Loading /home/esp/esp2/mission/GENOMAP.rb mission
@13:35:29.80 <MFBPCR> Extracting Nucleic Acid from extSrc
@13:35:29.83 SPE.hold 2:46:40,55C
@13:35:29.87 ASV.dial :SPEdelivery
@13:35:30.30 delay 20 seconds
@13:35:30.87 <bspe> Session Begun
@13:35:50.33 <MFBPCR> AFV.select :holdingCoil
@13:35:50.46 ATV.dial :air2
@13:35:51.74 AS.reconfigure ASslow1
@13:35:51.80 AS.seek 0.07ml
@13:36:06.29 delay 3 seconds
@13:36:09.32 ATV.dial :extSrc
@13:36:10.82 AS.seek 0.47ml
@13:37:31.65 delay 5 seconds
@13:37:36.73 Solid Phase Extraction Heater at 39C
@13:37:36.74 ATV.dial ASV
@13:37:37.62 ASV.dial :SPEdelivery
@13:37:37.71 AS.seek 0.042ml
@13:39:04.05 delay 10 seconds
@13:39:14.09 Rinsing external lysate line extSrc
@13:39:14.10 AFV.select :carrierSolution
@13:39:14.22 delay 2 seconds
@13:39:16.24 AS.reconfigure ASconfig
@13:39:16.30 AS.seek 1ml
@13:40:11.68 delay 1 second
@13:40:12.70 AFV.select :holdingCoil
@13:40:12.81 delay 1 second
@13:40:13.83 ATV.dial :extSrc
@13:40:14.66 AS.reconfigure ASnorm
@13:40:14.72 AS.seek 0ml
@13:41:05.40 ATV.dial :air2
@13:41:07.00 AS.reconfigure ASslow0
@13:41:07.06 AS.seek 0.05ml
@13:41:12.44 ATV.dial :extSrc
@13:41:14.02 AS.seek 0.01ml
@13:41:18.34 ATV.dial :waste
@13:41:19.74 AS.reconfigure ASconfig
@13:41:19.79 AS.seek 0ml
@13:41:21.02 ethanol wash
@13:41:21.03 ATV.dial :air2
@13:41:22.22 AS.reconfigure ASslow2
@13:41:22.28 AS.seek 0.01ml
@13:41:32.68 delay 3 seconds
@13:41:35.71 Pulling 0.4ml of Ethanol Wash
@13:41:35.72 ATV.dial :ethanol
@13:41:36.60 AS.reconfigure ASslow1
@13:41:36.66 AS.seek 0.41ml
@13:42:57.35 delay 3 seconds
@13:43:00.38 ATV.dial :air1
@13:43:00.80 delay 3 seconds
@13:43:03.87 AS.seek 0.435ml
@13:43:09.19 delay 3 seconds
@13:43:12.22 ATV.dial :ethanol
@13:43:12.86 AS.seek 0.42ml
@13:43:16.13 delay 3 seconds
@13:43:19.16 ATV.dial ASV
@13:43:20.55 AS.seek 0.093ml
@13:44:26.57 delay 3 seconds
@13:44:29.60 ATV.dial :waste2
@13:44:30.41 AS.reconfigure ASconfig
@13:44:30.46 AS.seek 0ml
@13:44:36.10 ATV.dial :air2
@13:44:36.53 AS.reconfigure ASslow1
@13:44:36.58 AS.seek 1ml
@13:47:57.99 delay 5 seconds
@13:48:03.02 ATV.dial ASV
@13:48:04.07 ASV.dial :SPEdelivery
@13:48:04.10 AS.seek 0.02ml
@13:51:21.46 delay 5 seconds
@13:51:26.49 ATV.dial :air2
@13:51:27.60 AS.seek 0.52ml
@13:53:08.44 delay 5 seconds
@13:53:13.47 ATV.dial ASV
@13:53:14.51 ASV.dial :wasteR
@13:53:14.91 AS.seek 0.3ml
@13:53:59.44 delay 20 seconds
@13:54:19.48 AS.seek 0.07ml
@13:55:06.01 delay 20 seconds
@13:55:26.04 ATV.dial :waste2
@13:55:26.85 AS.reconfigure ASconfig
@13:55:26.90 AS.seek 0ml
@13:55:31.21 Elution
@13:55:31.22 ATV.dial :air1
@13:55:32.41 AS.reconfigure ASslow1
@13:55:32.46 AS.seek 0.05ml
@13:55:42.82 delay 3 seconds
@13:55:45.85 ATV.dial :ElutionWater
@13:55:46.57 AS.seek 0.11ml
@13:55:58.93 delay 3 seconds
@13:56:01.96 ATV.dial ASV,via: :air2
@13:56:04.18 ASV.dial :SPEelution
@13:56:04.80 AFV.select :carrierSolution
@13:56:04.96 AS.reconfigure ASnorm
@13:56:05.02 AS.seek 0.76ml
@13:56:38.10 delay 3 seconds
@13:56:41.12 AFV.select :holdingCoil
@13:56:41.27 AS.reconfigure ASslow1
@13:56:41.33 AS.seek 0.475ml
@13:57:38.94 delay 5 seconds
@13:57:44.02 AS.reconfigure ASslow2
@13:57:44.08 AS.seek 0.44ml
@13:58:19.74 delay 1:00
@13:59:19.78 pushing eluate to holding coil
@13:59:19.85 AS.seek 0.28ml
@14:02:01.66 delay 30 seconds
@14:02:31.70 ATV.dial :waste,via: :air3
@14:02:33.52 AS.reconfigure ASconfig
@14:02:33.57 AS.seek 0ml
@14:02:49.99 Parking Bleach in LysateSource line and SPEcolumn
@14:02:50.00 ATV.dial :air3
@14:02:50.90 AS.reconfigure ASslow1
@14:02:50.95 AS.seek 0.03ml
@14:02:57.30 ATV.dial :ElutionWater
@14:02:57.94 AS.seek 0.06ml
@14:03:04.23 ATV.dial :air3
@14:03:04.88 AS.seek 0.07ml
@14:03:07.15 delay 2 seconds
@14:03:09.18 ATV.dial :bleach
@14:03:09.67 AS.seek 0.15ml
@14:03:26.19 delay 3 seconds
@14:03:29.22 ATV.dial :air3
@14:03:29.80 delay 3 seconds
@14:03:32.87 AS.seek 0.175ml
@14:03:38.16 delay 3 seconds
@14:03:41.19 ATV.dial :bleach
@14:03:41.82 AS.seek 0.16ml
@14:03:45.09 delay 3 seconds
@14:03:48.12 ATV.dial :extSrc
@14:03:48.77 AS.reconfigure ASconfig
@14:03:48.82 AS.seek 0.015ml
@14:03:57.46 ATV.dial :waste
@14:03:59.00 AS.seek 0ml
@14:04:00.15 ATV.dial :air3
@14:04:01.04 AS.reconfigure ASslow1
@14:04:01.10 AS.seek 0.01ml
@14:04:03.40 delay 2 seconds
@14:04:05.43 ATV.dial :bleach
@14:04:05.91 AS.seek 0.58ml
@14:06:00.88 delay 3 seconds
@14:06:03.91 ATV.dial :air3
@14:06:04.49 delay 3 seconds
@14:06:07.56 AS.seek 0.605ml
@14:06:12.88 delay 3 seconds
@14:06:15.91 ATV.dial :bleach
@14:06:16.54 AS.seek 0.59ml
@14:06:19.93 delay 3 seconds
@14:06:22.96 ATV.dial :ASV
@14:06:24.07 ASV.dial :wasteR
@14:06:24.71 AS.seek 0ml
@14:08:23.77 delay 1:00
@14:09:23.80 AFV.select :carrierSolution
@14:09:23.91 delay 2 seconds
@14:09:25.93 AS.reconfigure ASconfig
@14:09:25.98 AS.seek 1ml
@14:10:23.73 delay 1 second
@14:10:24.75 AFV.select :holdingCoil
@14:10:24.86 delay 1 second
@14:10:25.88 ATV.dial ASV
@14:10:25.89 ASV.dial :wasteR
@14:10:25.91 AS.reconfigure ASslow1
@14:10:25.98 AS.seek 0ml
@14:13:47.49 delay 20 seconds
@14:14:07.53 ATV.dial :air3
@14:14:09.13 AS.seek 0.59ml
@14:16:08.07 delay 3 seconds
@14:16:11.10 ATV.dial :ASV
@14:16:12.62 ASV.dial :wasteR
@14:16:12.71 AS.seek 0.09ml
@14:17:53.45 delay 30 seconds
@14:18:23.49 ATV.dial :extSrc
@14:18:24.32 delay 3 seconds
@14:18:27.35 ATV.dial :ElutionWater
@14:18:28.52 AS.seek 0.12ml
@14:18:34.88 ATV.dial :air3
@14:18:35.52 AS.seek 0.13ml
@14:18:37.79 delay 2 seconds
@14:18:39.82 ATV.dial :bleach
@14:18:40.30 AS.seek 0.23ml
@14:19:00.68 delay 3 seconds
@14:19:03.71 ATV.dial :air3
@14:19:04.29 delay 3 seconds
@14:19:07.36 AS.seek 0.255ml
@14:19:12.75 delay 3 seconds
@14:19:15.78 ATV.dial :bleach
@14:19:16.83 AS.seek 0.24ml
@14:19:20.10 delay 3 seconds
@14:19:23.13 ATV.dial :ASV
@14:19:24.23 ASV.dial :SPEdelivery
@14:19:24.63 AFV.select :carrierSolution
@14:19:24.75 delay 2 seconds
@14:19:26.77 AS.reconfigure ASconfig
@14:19:26.83 AS.seek 0.5ml
@14:19:42.09 delay 1 second
@14:19:43.11 AFV.select :holdingCoil
@14:19:43.26 AS.reconfigure ASslow1
@14:19:43.32 AS.seek 0.17ml
@14:20:50.00 SPE.hold 2:46:40,95C
@14:20:50.06 delay 3:00
@14:23:50.11 AFV.select :carrierSolution
@14:23:50.22 delay 2 seconds
@14:23:52.24 AS.reconfigure ASconfig
@14:23:52.29 AS.seek 1ml
@14:24:40.29 delay 1 second
@14:24:41.31 AFV.select :holdingCoil
@14:24:41.42 delay 1 second
@14:24:42.44 ASV.dial :wasteD
@14:24:43.56 AS.seek 0ml
@14:25:41.34 Priming Eluate
@14:25:41.35 AFV.select :carrierSolution
@14:25:41.48 delay 2 seconds
@14:25:43.50 AS.seek 0.5ml
@14:26:12.54 delay 1 second
@14:26:13.56 AFV.select :holdingCoil
@14:26:13.67 ATV.dial ASV
@14:26:13.68 ASV.dial :SPErecovery
@14:26:14.32 Swish #1 of 2
@14:26:14.40 AS.reconfigure ASslow0
@14:26:14.46 AS.seek 0.6ml
@14:26:24.91 AS.seek 0.5ml
@14:26:35.26 Swish #2 of 2
@14:26:35.34 AS.seek 0.6ml
@14:26:45.85 AS.seek 0.5ml
@14:26:56.23 delay 3 seconds
@14:26:59.30 AS.reconfigure ASslow1
@14:26:59.36 AS.seek 0.684ml
@14:27:36.66 delay 3 seconds
@14:27:39.69 Eluate primed
@14:27:39.70 ATV.dial :waste2
@14:27:40.39 AS.reconfigure ASnorm
@14:27:40.44 AS.seek 0ml
@14:28:15.24 SPE.coast
@14:28:15.32 ASV.dial :SPErecovery
@14:28:15.35 Setting up PCR for ACYPP2 -- writing /var/log/esp/ACYPP221apr1214h.pcr
@14:28:15.37 Building PCR Slug from ASV with MM1 mix, PP2 primer
@14:28:15.39 Priming with carrier
@14:28:15.41 ATV.dial ARV
@14:28:16.46 ARV.dial :carrier
@14:28:16.82 AS.seek 0.5ml
@14:28:42.40 AFV.select :carrierSolution
@14:28:42.53 delay 2 seconds
@14:28:44.55 AS.reconfigure ASconfig
@14:28:44.61 AS.seek 1ml
@14:29:13.62 delay 1 second
@14:29:14.64 AFV.select :holdingCoil
@14:29:14.75 delay 1 second
@14:29:15.77 ATV.dial :waste
@14:29:17.36 AS.reconfigure ASnorm
@14:29:17.42 AS.seek 0ml
@14:30:08.13 AFV.select :carrierSolution
@14:30:08.26 delay 2 seconds
@14:30:10.28 AS.reconfigure ASconfig
@14:30:10.34 AS.seek 0.5ml
@14:30:39.38 delay 1 second
@14:30:40.40 AFV.select :holdingCoil
@14:30:40.51 Building Slug
@14:30:40.52 ATV.dial :ARV
@14:30:42.24 delay 2 seconds
@14:30:44.27 ARV.dial :air1
@14:30:44.66 delay 2 seconds
@14:30:46.73 AS.reconfigure ASslow2
@14:30:46.79 AS.seek 0.506ml
@14:30:53.07 delay 2 seconds
@14:30:55.10 ARV.dial :MM1
@14:30:55.74 delay 2 seconds
@14:30:57.77 Adding MM1
@14:30:57.84 AS.seek 0.524ml
@14:31:16.16 delay 25 seconds
@14:31:41.19 ARV.dial :PP2
@14:31:41.61 Adding PP2
@14:31:41.62 delay 2 seconds
@14:31:43.70 AS.seek 0.53ml
@14:31:50.02 delay 10 seconds
@14:32:00.06 ARV.dial :air2
@14:32:00.50 adding ARV air
@14:32:00.51 delay 2 seconds
@14:32:02.59 AS.seek 0.536ml
@14:32:08.87 delay 5 seconds
@14:32:13.90 ARV.dial :carrier
@14:32:15.26 delay 7 seconds
@14:32:22.35 AS.seek 0.594ml
@14:33:20.90 delay 5 seconds
@14:33:25.93 ATV.dial :ASV
@14:33:26.35 delay 2 seconds
@14:33:28.43 AS.seek 0.6ml
@14:33:34.99 delay 4 seconds
@14:33:39.02 ATV.dial ARV
@14:33:39.60 delay 2 seconds
@14:33:41.63 ARV.dial :carrier
@14:33:41.64 delay 2 seconds
@14:33:43.72 AS.seek 0.65ml
@14:34:34.76 delay 4 seconds
@14:34:38.79 ATV.dial PCR
@14:34:39.41 ASV.dial :waste1
@14:34:40.23 ARV.dial :air1
@14:34:40.64 Positioning Slug by 0.324ml
@14:34:40.72 AS.seek 0.326ml
@14:40:06.65 delay 5 seconds
@14:40:11.68 ATV.dial ARV
@14:40:12.49 ASV.dial :PCRlock
@14:40:12.88 delay 5 seconds
@14:40:17.91 Running ACYPP2 Profile60CIQ2 thermocycling
@14:40:17.92 <PCR> PCR.program Profile60CIQ2
@14:40:18.04 Profile60CIQ2 should complete in < 1:47:50
@14:40:18.05 PCR.run 2:19:47.5
@16:08:52.64 PCR.off
@16:08:53.59 <MFBPCR> Running ACYPP2 MeltProfile60 thermocycling
@16:08:53.61 <PCR> PCR.program MeltProfile60
@16:08:53.71 MeltProfile60 should complete in < 11:55
@16:08:53.72 PCR.run 19:53.75
@16:16:31.67 PCR.off
@16:16:32.62 <MFBPCR> PCR for ACYPP2 completed -- closed /var/log/esp/ACYPP221apr1214h.pcr
@16:16:32.70 Cleaning PCR
@16:16:32.71 AFV.select :carrierSolution
@16:16:32.83 delay 2 seconds
@16:16:34.85 AS.reconfigure ASconfig
@16:16:34.91 AS.seek 0.5ml
@16:16:45.18 delay 1 second
@16:16:46.20 AFV.select :holdingCoil
@16:16:46.31 ASV.dial :waste1
@16:16:46.71 ATV.dial :air3
@16:16:48.08 AS.reconfigure ASslow1
@16:16:48.14 AS.seek 0.51ml
@16:16:50.52 delay 2 seconds
@16:16:52.54 ATV.dial :bleach
@16:16:53.18 AS.seek 0.81ml
@16:17:53.79 delay 3 seconds
@16:17:56.82 ATV.dial :air3
@16:17:57.38 delay 3 seconds
@16:18:00.45 AS.seek 0.835ml
@16:18:05.77 delay 3 seconds
@16:18:08.80 ATV.dial :bleach
@16:18:09.43 AS.seek 0.82ml
@16:18:12.74 delay 3 seconds
@16:18:15.77 ARV.dial :waste
@16:18:16.40 delay 3 seconds
@16:18:19.43 ATV.dial :ARV
@16:18:20.36 AS.reconfigure ASconfig
@16:18:20.42 AS.seek 0.47ml
@16:18:40.80 AFV.select :carrierSolution
@16:18:40.92 delay 2 seconds
@16:18:42.94 AS.seek 1ml
@16:19:13.66 delay 1 second
@16:19:14.69 AFV.select :holdingCoil
@16:19:14.79 delay 1 second
@16:19:15.81 AS.reconfigure ASfast
@16:19:15.86 AS.seek 0ml
@16:19:43.20 AFV.select :carrierSolution
@16:19:43.32 delay 2 seconds
@16:19:45.34 AS.reconfigure ASconfig
@16:19:45.39 AS.seek 1ml
@16:20:43.20 delay 1 second
@16:20:44.22 AFV.select :holdingCoil
@16:20:44.33 delay 1 second
@16:20:45.35 AS.reconfigure ASfast
@16:20:45.41 AS.seek 0ml
@16:21:12.76 AFV.select :carrierSolution
@16:21:12.88 delay 2 seconds
@16:21:14.90 AS.reconfigure ASconfig
@16:21:14.95 AS.seek 1ml
@16:22:12.76 delay 1 second
@16:22:13.78 AFV.select :holdingCoil
@16:22:13.89 delay 1 second
@16:22:14.91 AS.reconfigure ASfast
@16:22:14.97 AS.seek 0ml
@16:22:42.32 ARV.dial between carrier and air1
@16:22:42.85 ATV.dial :air3
@16:22:44.21 AS.reconfigure ASslow1
@16:22:44.27 AS.seek 0.01ml
@16:22:46.73 delay 2 seconds
@16:22:48.76 ATV.dial :bleach
@16:22:49.40 AS.seek 0.81ml
@16:25:30.55 delay 3 seconds
@16:25:33.58 ATV.dial :air3
@16:25:34.16 delay 3 seconds
@16:25:37.23 AS.seek 0.835ml
@16:25:42.62 delay 3 seconds
@16:25:45.65 ATV.dial :bleach
@16:25:46.29 AS.seek 0.82ml
@16:25:49.59 delay 3 seconds
@16:25:52.62 ATV.dial PCR
@16:25:53.03 AS.reconfigure ASconfig
@16:25:53.08 AS.seek 0ml
@16:26:40.49 PCR.program :bleachProfile
@16:26:40.56 PCR program should complete in < 2:45
@16:26:40.58 PCR.run 10:00
@16:29:36.79 PCR.off
@16:29:36.84 AFV.select :carrierSolution
@16:29:36.95 delay 2 seconds
@16:29:38.97 AS.seek 1ml
@16:30:36.73 delay 1 second
@16:30:37.75 AFV.select :holdingCoil
@16:30:37.86 delay 1 second
@16:30:38.88 AS.reconfigure ASfast
@16:30:38.94 AS.seek 0ml
@16:31:06.26 delay 3 seconds
@16:31:09.28 AFV.select :carrierSolution
@16:31:09.39 delay 2 seconds
@16:31:11.41 AS.reconfigure ASconfig
@16:31:11.47 AS.seek 1ml
@16:32:09.23 delay 1 second
@16:32:10.25 AFV.select :holdingCoil
@16:32:10.36 delay 1 second
@16:32:11.38 AS.reconfigure ASfast
@16:32:11.44 AS.seek 0ml
@16:32:38.77 delay 3 seconds
@16:32:41.79 AFV.select :carrierSolution
@16:32:41.90 delay 2 seconds
@16:32:43.92 AS.reconfigure ASconfig
@16:32:43.98 AS.seek 1ml
@16:33:41.74 delay 1 second
@16:33:42.76 AFV.select :holdingCoil
@16:33:42.87 delay 1 second
@16:33:43.89 AS.reconfigure ASfast
@16:33:43.95 AS.seek 0ml
@16:34:11.30 delay 3 seconds
@16:34:14.32 AFV.select :carrierSolution
@16:34:14.43 delay 2 seconds
@16:34:16.45 AS.reconfigure ASconfig
@16:34:16.51 AS.seek 1ml
@16:35:14.27 delay 1 second
@16:35:15.29 AFV.select :holdingCoil
@16:35:15.40 delay 1 second
@16:35:16.42 AS.reconfigure ASfast
@16:35:16.47 AS.seek 0ml
@16:35:43.82 delay 3 seconds
@16:35:46.84 AFV.select :carrierSolution
@16:35:46.95 delay 2 seconds
@16:35:48.97 AS.reconfigure ASconfig
@16:35:49.02 AS.seek 1ml
@16:36:46.76 delay 1 second
@16:36:47.78 AFV.select :holdingCoil
@16:36:47.89 delay 1 second
@16:36:48.91 AS.reconfigure ASnorm
@16:36:48.96 AS.seek 0ml
@16:37:39.68 Setting up PCR for AOPP1 -- writing /var/log/esp/AOPP121apr1216h.pcr
@16:37:39.70 Building PCR Slug from ASV with MM1 mix, PP1 primer
@16:37:39.72 Priming with carrier
@16:37:39.73 ATV.dial ARV
@16:37:40.38 ARV.dial :carrier
@16:37:40.72 AS.seek 0.5ml
@16:38:06.30 AFV.select :carrierSolution
@16:38:06.43 delay 2 seconds
@16:38:08.45 AS.reconfigure ASconfig
@16:38:08.51 AS.seek 1ml
@16:38:37.54 delay 1 second
@16:38:38.57 AFV.select :holdingCoil
@16:38:38.67 delay 1 second
@16:38:39.69 ATV.dial :waste
@16:38:41.46 AS.reconfigure ASnorm
@16:38:41.52 AS.seek 0ml
@16:39:32.18 AFV.select :carrierSolution
@16:39:32.30 delay 2 seconds
@16:39:34.32 AS.reconfigure ASconfig
@16:39:34.38 AS.seek 0.5ml
@16:40:03.40 delay 1 second
@16:40:04.42 AFV.select :holdingCoil
@16:40:04.53 Building Slug
@16:40:04.54 ATV.dial :ARV
@16:40:06.27 delay 2 seconds
@16:40:08.30 ARV.dial :air1
@16:40:08.72 delay 2 seconds
@16:40:10.79 AS.reconfigure ASslow2
@16:40:10.85 AS.seek 0.506ml
@16:40:17.48 delay 2 seconds
@16:40:19.51 ARV.dial :MM1
@16:40:20.13 delay 2 seconds
@16:40:22.16 Adding MM1
@16:40:22.23 AS.seek 0.524ml
@16:40:40.43 delay 25 seconds
@16:41:05.46 ARV.dial :PP1
@16:41:05.86 Adding PP1
@16:41:05.87 delay 2 seconds
@16:41:07.94 AS.seek 0.53ml
@16:41:14.34 delay 10 seconds
@16:41:24.38 ARV.dial :air1
@16:41:24.80 adding ARV air
@16:41:24.81 delay 2 seconds
@16:41:26.88 AS.seek 0.536ml
@16:41:33.30 delay 5 seconds
@16:41:38.33 ARV.dial :carrier
@16:41:38.74 delay 7 seconds
@16:41:45.83 AS.seek 0.594ml
@16:42:44.38 delay 5 seconds
@16:42:49.41 ATV.dial :ASV
@16:42:49.82 delay 2 seconds
@16:42:51.90 AS.seek 0.6ml
@16:42:58.35 delay 4 seconds
@16:43:02.38 ATV.dial ARV
@16:43:02.96 delay 2 seconds
@16:43:04.99 ARV.dial :carrier
@16:43:05.00 delay 2 seconds
@16:43:07.07 AS.seek 0.65ml
@16:43:57.77 delay 4 seconds
@16:44:01.80 ATV.dial PCR
@16:44:02.43 ASV.dial :waste1
@16:44:02.45 ARV.dial :air1
@16:44:02.87 Positioning Slug by 0.324ml
@16:44:02.95 AS.seek 0.326ml
@16:49:29.20 delay 5 seconds
@16:49:34.23 ATV.dial ARV
@16:49:35.04 ASV.dial :PCRlock
@16:49:35.45 delay 5 seconds
@16:49:40.48 Running AOPP1 Profile60CIQ1 thermocycling
@16:49:40.49 <PCR> PCR.program Profile60CIQ1
@16:49:40.61 Profile60CIQ1 should complete in < 1:56:15
@16:49:40.62 PCR.run 2:30:18.75
@18:28:37.12 PCR.off
@18:28:37.70 <MFBPCR> Running AOPP1 MeltProfile60 thermocycling
@18:28:37.71 <PCR> PCR.program MeltProfile60
@18:28:37.82 MeltProfile60 should complete in < 11:55
@18:28:37.83 PCR.run 19:53.75
@18:36:06.85 PCR.off
@18:36:07.79 <MFBPCR> PCR for AOPP1 completed -- closed /var/log/esp/AOPP121apr1216h.pcr
@18:36:07.87 Cleaning PCR
@18:36:07.88 AFV.select :carrierSolution
@18:36:08.00 delay 2 seconds
@18:36:10.02 AS.reconfigure ASconfig
@18:36:10.08 AS.seek 0.5ml
@18:36:20.38 delay 1 second
@18:36:21.40 AFV.select :holdingCoil
@18:36:21.51 ASV.dial :waste1
@18:36:21.90 ATV.dial :air3
@18:36:23.26 AS.reconfigure ASslow1
@18:36:23.32 AS.seek 0.51ml
@18:36:25.69 delay 2 seconds
@18:36:27.72 ATV.dial :bleach
@18:36:28.37 AS.seek 0.81ml
@18:37:28.99 delay 3 seconds
@18:37:32.02 ATV.dial :air3
@18:37:32.60 delay 3 seconds
@18:37:35.67 AS.seek 0.835ml
@18:37:41.02 delay 3 seconds
@18:37:44.05 ATV.dial :bleach
@18:37:44.69 AS.seek 0.82ml
@18:37:48.07 delay 3 seconds
@18:37:51.10 ARV.dial :waste
@18:37:51.73 delay 3 seconds
@18:37:54.76 ATV.dial :ARV
@18:37:55.68 AS.reconfigure ASconfig
@18:37:55.73 AS.seek 0.47ml
@18:38:16.13 AFV.select :carrierSolution
@18:38:16.25 delay 2 seconds
@18:38:18.27 AS.seek 1ml
@18:38:49.05 delay 1 second
@18:38:50.07 AFV.select :holdingCoil
@18:38:50.18 delay 1 second
@18:38:51.20 AS.reconfigure ASfast
@18:38:51.26 AS.seek 0ml
@18:39:18.59 AFV.select :carrierSolution
@18:39:18.71 delay 2 seconds
@18:39:20.73 AS.reconfigure ASconfig
@18:39:20.78 AS.seek 1ml
@18:40:18.54 delay 1 second
@18:40:19.56 AFV.select :holdingCoil
@18:40:19.67 delay 1 second
@18:40:20.69 AS.reconfigure ASfast
@18:40:20.75 AS.seek 0ml
@18:40:48.07 AFV.select :carrierSolution
@18:40:48.19 delay 2 seconds
@18:40:50.21 AS.reconfigure ASconfig
@18:40:50.26 AS.seek 1ml
@18:41:48.01 delay 1 second
@18:41:49.03 AFV.select :holdingCoil
@18:41:49.14 delay 1 second
@18:41:50.16 AS.reconfigure ASfast
@18:41:50.22 AS.seek 0ml
@18:42:17.54 ARV.dial between carrier and air1
@18:42:18.07 ATV.dial :air3
@18:42:19.43 AS.reconfigure ASslow1
@18:42:19.49 AS.seek 0.01ml
@18:42:21.82 delay 2 seconds
@18:42:23.85 ATV.dial :bleach
@18:42:24.49 AS.seek 0.81ml
@18:45:05.62 delay 3 seconds
@18:45:08.65 ATV.dial :air3
@18:45:09.23 delay 3 seconds
@18:45:12.30 AS.seek 0.835ml
@18:45:17.69 delay 3 seconds
@18:45:20.72 ATV.dial :bleach
@18:45:21.37 AS.seek 0.82ml
@18:45:24.79 delay 3 seconds
@18:45:27.82 ATV.dial PCR
@18:45:28.25 AS.reconfigure ASconfig
@18:45:28.30 AS.seek 0ml
@18:46:15.73 PCR.program :bleachProfile
@18:46:15.80 PCR program should complete in < 2:45
@18:46:15.82 PCR.run 10:00
@18:49:12.03 PCR.off
@18:49:12.07 AFV.select :carrierSolution
@18:49:12.18 delay 2 seconds
@18:49:14.20 AS.seek 1ml
@18:50:11.95 delay 1 second
@18:50:12.97 AFV.select :holdingCoil
@18:50:13.08 delay 1 second
@18:50:14.10 AS.reconfigure ASfast
@18:50:14.16 AS.seek 0ml
@18:50:41.51 delay 3 seconds
@18:50:44.53 AFV.select :carrierSolution
@18:50:44.64 delay 2 seconds
@18:50:46.66 AS.reconfigure ASconfig
@18:50:46.72 AS.seek 1ml
@18:51:44.46 delay 1 second
@18:51:45.48 AFV.select :holdingCoil
@18:51:45.59 delay 1 second
@18:51:46.61 AS.reconfigure ASfast
@18:51:46.67 AS.seek 0ml
@18:52:13.99 delay 3 seconds
@18:52:17.01 AFV.select :carrierSolution
@18:52:17.12 delay 2 seconds
@18:52:19.14 AS.reconfigure ASconfig
@18:52:19.19 AS.seek 1ml
@18:53:16.96 delay 1 second
@18:53:17.98 AFV.select :holdingCoil
@18:53:18.09 delay 1 second
@18:53:19.11 AS.reconfigure ASfast
@18:53:19.17 AS.seek 0ml
@18:53:46.49 delay 3 seconds
@18:53:49.51 AFV.select :carrierSolution
@18:53:49.65 delay 2 seconds
@18:53:51.68 AS.reconfigure ASconfig
@18:53:51.73 AS.seek 1ml
@18:54:49.52 delay 1 second
@18:54:50.54 AFV.select :holdingCoil
@18:54:50.65 delay 1 second
@18:54:51.67 AS.reconfigure ASfast
@18:54:51.73 AS.seek 0ml
@18:55:19.06 delay 3 seconds
@18:55:22.08 AFV.select :carrierSolution
@18:55:22.19 delay 2 seconds
@18:55:24.21 AS.reconfigure ASconfig
@18:55:24.27 AS.seek 1ml
@18:56:22.06 delay 1 second
@18:56:23.08 AFV.select :holdingCoil
@18:56:23.19 delay 1 second
@18:56:24.21 AS.reconfigure ASnorm
@18:56:24.26 AS.seek 0ml
@18:57:15.04 Setting up PCR for AARPP4 -- writing /var/log/esp/AARPP421apr1218h.pcr
@18:57:15.06 Building PCR Slug from ASV with MM2 mix, PP4 primer
@18:57:15.08 Priming with carrier
@18:57:15.09 ATV.dial ARV
@18:57:15.85 ARV.dial :carrier
@18:57:16.19 AS.seek 0.5ml
@18:57:41.74 AFV.select :carrierSolution
@18:57:41.86 delay 2 seconds
@18:57:43.88 AS.reconfigure ASconfig
@18:57:43.94 AS.seek 1ml
@18:58:12.96 delay 1 second
@18:58:13.98 AFV.select :holdingCoil
@18:58:14.09 delay 1 second
@18:58:15.11 ATV.dial :waste
@18:58:16.88 AS.reconfigure ASnorm
@18:58:16.94 AS.seek 0ml
@18:59:07.66 AFV.select :carrierSolution
@18:59:07.78 delay 2 seconds
@18:59:09.80 AS.reconfigure ASconfig
@18:59:09.86 AS.seek 0.5ml
@18:59:38.87 delay 1 second
@18:59:39.89 AFV.select :holdingCoil
@18:59:40.00 Building Slug
@18:59:40.01 ATV.dial :ARV
@18:59:41.74 delay 2 seconds
@18:59:43.77 ARV.dial :air1
@18:59:44.18 delay 2 seconds
@18:59:46.25 AS.reconfigure ASslow2
@18:59:46.31 AS.seek 0.506ml
@18:59:52.68 delay 2 seconds
@18:59:54.71 ARV.dial :MM2
@18:59:56.29 delay 2 seconds
@18:59:58.32 Adding MM2
@18:59:58.39 AS.seek 0.524ml
@19:00:16.74 delay 25 seconds
@19:00:41.77 ARV.dial :PP4
@19:00:42.19 Adding PP4
@19:00:42.20 delay 2 seconds
@19:00:44.27 AS.seek 0.53ml
@19:00:50.65 delay 10 seconds
@19:01:00.69 ARV.dial :air3
@19:01:01.11 adding ARV air
@19:01:01.12 delay 2 seconds
@19:01:03.19 AS.seek 0.536ml
@19:01:09.37 delay 5 seconds
@19:01:14.40 ARV.dial :carrier
@19:01:16.13 delay 7 seconds
@19:01:23.21 AS.seek 0.594ml
@19:02:22.04 delay 5 seconds
@19:02:27.07 ATV.dial :ASV
@19:02:27.49 delay 2 seconds
@19:02:29.56 AS.seek 0.6ml
@19:02:35.98 delay 4 seconds
@19:02:40.01 ATV.dial ARV
@19:02:40.60 delay 2 seconds
@19:02:42.63 ARV.dial :carrier
@19:02:42.64 delay 2 seconds
@19:02:44.72 AS.seek 0.65ml
@19:03:35.59 delay 4 seconds
@19:03:39.62 ATV.dial PCR
@19:03:40.26 ASV.dial :waste1
@19:03:40.28 ARV.dial :air1
@19:03:40.69 Positioning Slug by 0.324ml
@19:03:40.78 AS.seek 0.326ml
@19:09:06.81 delay 5 seconds
@19:09:11.84 ATV.dial ARV
@19:09:12.65 ASV.dial :PCRlock
@19:09:13.06 delay 5 seconds
@19:09:18.09 Running AARPP4 Profile58CIQ2 thermocycling
@19:09:18.10 <PCR> PCR.program Profile58CIQ2
@19:09:18.23 Profile58CIQ2 should complete in < 1:36:15
@19:09:18.24 PCR.run 2:05:18.75
@20:29:56.36 PCR.off
@20:29:56.93 <MFBPCR> Running AARPP4 MeltProfile58 thermocycling
@20:29:56.95 <PCR> PCR.program MeltProfile58
@20:29:57.05 MeltProfile58 should complete in < 12:33
@20:29:57.06 PCR.run 20:41.25
@20:37:39.05 PCR.off
@20:37:40.04 <MFBPCR> PCR for AARPP4 completed -- closed /var/log/esp/AARPP421apr1218h.pcr
@20:37:40.12 Cleaning PCR
@20:37:40.13 AFV.select :carrierSolution
@20:37:40.25 delay 2 seconds
@20:37:42.27 AS.reconfigure ASconfig
@20:37:42.32 AS.seek 0.5ml
@20:37:52.62 delay 1 second
@20:37:53.64 AFV.select :holdingCoil
@20:37:53.75 ASV.dial :waste1
@20:37:54.15 ATV.dial :air3
@20:37:55.51 AS.reconfigure ASslow1
@20:37:55.57 AS.seek 0.51ml
@20:37:57.96 delay 2 seconds
@20:37:59.99 ATV.dial :bleach
@20:38:00.63 AS.seek 0.81ml
@20:39:01.24 delay 3 seconds
@20:39:04.27 ATV.dial :air3
@20:39:04.85 delay 3 seconds
@20:39:07.92 AS.seek 0.835ml
@20:39:13.21 delay 3 seconds
@20:39:16.24 ATV.dial :bleach
@20:39:16.88 AS.seek 0.82ml
@20:39:20.14 delay 3 seconds
@20:39:23.17 ARV.dial :waste
@20:39:23.79 delay 3 seconds
@20:39:26.82 ATV.dial :ARV
@20:39:27.74 AS.reconfigure ASconfig
@20:39:27.79 AS.seek 0.47ml
@20:39:48.18 AFV.select :carrierSolution
@20:39:48.30 delay 2 seconds
@20:39:50.32 AS.seek 1ml
@20:40:21.07 delay 1 second
@20:40:22.09 AFV.select :holdingCoil
@20:40:22.20 delay 1 second
@20:40:23.22 AS.reconfigure ASfast
@20:40:23.28 AS.seek 0ml
@20:40:50.63 AFV.select :carrierSolution
@20:40:50.75 delay 2 seconds
@20:40:52.77 AS.reconfigure ASconfig
@20:40:52.82 AS.seek 1ml
@20:41:50.57 delay 1 second
@20:41:51.59 AFV.select :holdingCoil
@20:41:51.70 delay 1 second
@20:41:52.72 AS.reconfigure ASfast
@20:41:52.78 AS.seek 0ml
@20:42:20.09 AFV.select :carrierSolution
@20:42:20.21 delay 2 seconds
@20:42:22.23 AS.reconfigure ASconfig
@20:42:22.28 AS.seek 1ml
@20:43:20.04 delay 1 second
@20:43:21.06 AFV.select :holdingCoil
@20:43:21.17 delay 1 second
@20:43:22.19 AS.reconfigure ASfast
@20:43:22.25 AS.seek 0ml
@20:43:49.57 ARV.dial between carrier and air1
@20:43:50.09 ATV.dial :air3
@20:43:51.45 AS.reconfigure ASslow1
@20:43:51.50 AS.seek 0.01ml
@20:43:53.81 delay 2 seconds
@20:43:55.84 ATV.dial :bleach
@20:43:56.48 AS.seek 0.81ml
@20:46:37.78 delay 3 seconds
@20:46:40.81 ATV.dial :air3
@20:46:41.39 delay 3 seconds
@20:46:44.46 AS.seek 0.835ml
@20:46:49.88 delay 3 seconds
@20:46:52.91 ATV.dial :bleach
@20:46:53.55 AS.seek 0.82ml
@20:46:57.23 delay 3 seconds
@20:47:00.26 ATV.dial PCR
@20:47:00.69 AS.reconfigure ASconfig
@20:47:00.74 AS.seek 0ml
@20:47:48.15 PCR.program :bleachProfile
@20:47:48.23 PCR program should complete in < 2:45
@20:47:48.24 PCR.run 10:00
@20:50:44.03 PCR.off
@20:50:44.08 AFV.select :carrierSolution
@20:50:44.19 delay 2 seconds
@20:50:46.21 AS.seek 1ml
@20:51:43.99 delay 1 second
@20:51:45.01 AFV.select :holdingCoil
@20:51:45.12 delay 1 second
@20:51:46.14 AS.reconfigure ASfast
@20:51:46.19 AS.seek 0ml
@20:52:13.53 delay 3 seconds
@20:52:16.55 AFV.select :carrierSolution
@20:52:16.66 delay 2 seconds
@20:52:18.68 AS.reconfigure ASconfig
@20:52:18.74 AS.seek 1ml
@20:53:16.51 delay 1 second
@20:53:17.53 AFV.select :holdingCoil
@20:53:17.64 delay 1 second
@20:53:18.66 AS.reconfigure ASfast
@20:53:18.72 AS.seek 0ml
@20:53:46.07 delay 3 seconds
@20:53:49.09 AFV.select :carrierSolution
@20:53:49.20 delay 2 seconds
@20:53:51.22 AS.reconfigure ASconfig
@20:53:51.28 AS.seek 1ml
@20:54:49.04 delay 1 second
@20:54:50.06 AFV.select :holdingCoil
@20:54:50.17 delay 1 second
@20:54:51.19 AS.reconfigure ASfast
@20:54:51.25 AS.seek 0ml
@20:55:18.60 delay 3 seconds
@20:55:21.62 AFV.select :carrierSolution
@20:55:21.73 delay 2 seconds
@20:55:23.75 AS.reconfigure ASconfig
@20:55:23.81 AS.seek 1ml
@20:56:21.56 delay 1 second
@20:56:22.58 AFV.select :holdingCoil
@20:56:22.69 delay 1 second
@20:56:23.71 AS.reconfigure ASfast
@20:56:23.77 AS.seek 0ml
@20:56:51.10 delay 3 seconds
@20:56:54.12 AFV.select :carrierSolution
@20:56:54.23 delay 2 seconds
@20:56:56.25 AS.reconfigure ASconfig
@20:56:56.31 AS.seek 1ml
@20:57:54.07 delay 1 second
@20:57:55.09 AFV.select :holdingCoil
@20:57:55.20 delay 1 second
@20:57:56.22 AS.reconfigure ASnorm
@20:57:56.27 AS.seek 0ml
@20:58:46.99 Setting up PCR for AALPP3 -- writing /var/log/esp/AALPP321apr1220h.pcr
@20:58:47.00 Building PCR Slug from ASV with MM2 mix, PP3 primer
@20:58:47.03 Priming with carrier
@20:58:47.04 ATV.dial ARV
@20:58:47.68 ARV.dial :carrier
@20:58:48.02 AS.seek 0.5ml
@20:59:13.59 AFV.select :carrierSolution
@20:59:13.71 delay 2 seconds
@20:59:15.73 AS.reconfigure ASconfig
@20:59:15.79 AS.seek 1ml
@20:59:44.81 delay 1 second
@20:59:45.83 AFV.select :holdingCoil
@20:59:45.94 delay 1 second
@20:59:46.96 ATV.dial :waste
@20:59:49.13 AS.reconfigure ASnorm
@20:59:49.19 AS.seek 0ml
@21:00:39.90 AFV.select :carrierSolution
@21:00:40.03 delay 2 seconds
@21:00:42.05 AS.reconfigure ASconfig
@21:00:42.11 AS.seek 0.5ml
@21:01:11.15 delay 1 second
@21:01:12.17 AFV.select :holdingCoil
@21:01:12.28 Building Slug
@21:01:12.29 ATV.dial :ARV
@21:01:14.03 delay 2 seconds
@21:01:16.06 ARV.dial :air1
@21:01:16.47 delay 2 seconds
@21:01:18.54 AS.reconfigure ASslow2
@21:01:18.60 AS.seek 0.506ml
@21:01:25.15 delay 2 seconds
@21:01:27.18 ARV.dial :MM2
@21:01:28.76 delay 2 seconds
@21:01:30.79 Adding MM2
@21:01:30.88 AS.seek 0.524ml
@21:01:49.20 delay 25 seconds
@21:02:14.23 ARV.dial :PP3
@21:02:14.62 Adding PP3
@21:02:14.64 delay 2 seconds
@21:02:16.71 AS.seek 0.53ml
@21:02:23.09 delay 10 seconds
@21:02:33.13 ARV.dial :air2
@21:02:33.54 adding ARV air
@21:02:33.56 delay 2 seconds
@21:02:35.63 AS.seek 0.536ml
@21:02:41.99 delay 5 seconds
@21:02:47.02 ARV.dial :carrier
@21:02:48.37 delay 7 seconds
@21:02:55.45 AS.seek 0.594ml
@21:03:54.09 delay 5 seconds
@21:03:59.12 ATV.dial :ASV
@21:03:59.54 delay 2 seconds
@21:04:01.61 AS.seek 0.6ml
@21:04:08.25 delay 4 seconds
@21:04:12.28 ATV.dial ARV
@21:04:12.85 delay 2 seconds
@21:04:14.88 ARV.dial :carrier
@21:04:14.89 delay 2 seconds
@21:04:16.96 AS.seek 0.65ml
@21:05:07.73 delay 4 seconds
@21:05:11.76 ATV.dial PCR
@21:05:12.39 ASV.dial :waste1
@21:05:12.41 ARV.dial :air1
@21:05:12.83 Positioning Slug by 0.324ml
@21:05:12.92 AS.seek 0.326ml
@21:10:39.17 delay 5 seconds
@21:10:44.20 ATV.dial ARV
@21:10:45.01 ASV.dial :PCRlock
@21:10:45.42 delay 5 seconds
@21:10:50.45 Running AALPP3 Profile58CIQ2 thermocycling
@21:10:50.46 <PCR> PCR.program Profile58CIQ2
@21:10:50.58 Profile58CIQ2 should complete in < 1:36:15
@21:10:50.59 PCR.run 2:05:18.75
@22:31:14.57 PCR.off
@22:31:15.14 <MFBPCR> Running AALPP3 MeltProfile58 thermocycling
@22:31:15.16 <PCR> PCR.program MeltProfile58
@22:31:15.26 MeltProfile58 should complete in < 12:33
@22:31:15.27 PCR.run 20:41.25
@22:38:56.71 PCR.off
@22:38:57.71 <MFBPCR> PCR for AALPP3 completed -- closed /var/log/esp/AALPP321apr1220h.pcr
@22:38:57.78 Cleaning PCR
@22:38:57.79 AFV.select :carrierSolution
@22:38:57.91 delay 2 seconds
@22:38:59.93 AS.reconfigure ASconfig
@22:38:59.98 AS.seek 0.5ml
@22:39:10.34 delay 1 second
@22:39:11.36 AFV.select :holdingCoil
@22:39:11.47 ASV.dial :waste1
@22:39:11.86 ATV.dial :air3
@22:39:13.21 AS.reconfigure ASslow1
@22:39:13.27 AS.seek 0.51ml
@22:39:15.63 delay 2 seconds
@22:39:17.66 ATV.dial :bleach
@22:39:18.29 AS.seek 0.81ml
@22:40:19.00 delay 3 seconds
@22:40:22.03 ATV.dial :air3
@22:40:22.61 delay 3 seconds
@22:40:25.68 AS.seek 0.835ml
@22:40:31.02 delay 3 seconds
@22:40:34.05 ATV.dial :bleach
@22:40:34.70 AS.seek 0.82ml
@22:40:38.03 delay 3 seconds
@22:40:41.06 ARV.dial :waste
@22:40:41.70 delay 3 seconds
@22:40:44.73 ATV.dial :ARV
@22:40:45.65 AS.reconfigure ASconfig
@22:40:45.70 AS.seek 0.47ml
@22:41:06.12 AFV.select :carrierSolution
@22:41:06.24 delay 2 seconds
@22:41:08.26 AS.seek 1ml
@22:41:39.00 delay 1 second
@22:41:40.02 AFV.select :holdingCoil
@22:41:40.13 delay 1 second
@22:41:41.15 AS.reconfigure ASfast
@22:41:41.21 AS.seek 0ml
@22:42:08.53 AFV.select :carrierSolution
@22:42:08.65 delay 2 seconds
@22:42:10.67 AS.reconfigure ASconfig
@22:42:10.72 AS.seek 1ml
@22:43:08.89 delay 1 second
@22:43:09.91 AFV.select :holdingCoil
@22:43:10.02 delay 1 second
@22:43:11.04 AS.reconfigure ASfast
@22:43:11.10 AS.seek 0ml
@22:43:38.45 AFV.select :carrierSolution
@22:43:38.57 delay 2 seconds
@22:43:40.59 AS.reconfigure ASconfig
@22:43:40.64 AS.seek 1ml
@22:44:38.40 delay 1 second
@22:44:39.42 AFV.select :holdingCoil
@22:44:39.53 delay 1 second
@22:44:40.55 AS.reconfigure ASfast
@22:44:40.61 AS.seek 0ml
@22:45:07.95 ARV.dial between carrier and air1
@22:45:08.49 ATV.dial :air3
@22:45:09.82 AS.reconfigure ASslow1
@22:45:09.88 AS.seek 0.01ml
@22:45:12.20 delay 2 seconds
@22:45:14.23 ATV.dial :bleach
@22:45:14.87 AS.seek 0.81ml
@22:47:56.00 delay 3 seconds
@22:47:59.03 ATV.dial :air3
@22:47:59.61 delay 3 seconds
@22:48:02.68 AS.seek 0.835ml
@22:48:08.02 delay 3 seconds
@22:48:11.05 ATV.dial :bleach
@22:48:11.70 AS.seek 0.82ml
@22:48:15.00 delay 3 seconds
@22:48:18.03 ATV.dial PCR
@22:48:18.44 AS.reconfigure ASconfig
@22:48:18.49 AS.seek 0ml
@22:49:05.90 PCR.program :bleachProfile
@22:49:05.97 PCR program should complete in < 2:45
@22:49:05.98 PCR.run 10:00
@22:52:04.80 PCR.off
@22:52:04.84 AFV.select :carrierSolution
@22:52:04.95 delay 2 seconds
@22:52:06.97 AS.seek 1ml
@22:53:04.72 delay 1 second
@22:53:05.74 AFV.select :holdingCoil
@22:53:05.85 delay 1 second
@22:53:06.87 AS.reconfigure ASfast
@22:53:06.93 AS.seek 0ml
@22:53:34.26 delay 3 seconds
@22:53:37.28 AFV.select :carrierSolution
@22:53:37.39 delay 2 seconds
@22:53:39.41 AS.reconfigure ASconfig
@22:53:39.48 AS.seek 1ml
@22:54:37.23 delay 1 second
@22:54:38.25 AFV.select :holdingCoil
@22:54:38.36 delay 1 second
@22:54:39.38 AS.reconfigure ASfast
@22:54:39.44 AS.seek 0ml
@22:55:06.76 delay 3 seconds
@22:55:09.78 AFV.select :carrierSolution
@22:55:09.89 delay 2 seconds
@22:55:11.91 AS.reconfigure ASconfig
@22:55:11.97 AS.seek 1ml
@22:56:09.73 delay 1 second
@22:56:10.75 AFV.select :holdingCoil
@22:56:10.86 delay 1 second
@22:56:11.88 AS.reconfigure ASfast
@22:56:12.35 AS.seek 0ml
@22:56:39.68 delay 3 seconds
@22:56:42.70 AFV.select :carrierSolution
@22:56:42.81 delay 2 seconds
@22:56:44.83 AS.reconfigure ASconfig
@22:56:44.90 AS.seek 1ml
@22:57:42.65 delay 1 second
@22:57:43.67 AFV.select :holdingCoil
@22:57:43.78 delay 1 second
@22:57:44.80 AS.reconfigure ASfast
@22:57:44.86 AS.seek 0ml
@22:58:12.20 delay 3 seconds
@22:58:15.22 AFV.select :carrierSolution
@22:58:15.33 delay 2 seconds
@22:58:17.35 AS.reconfigure ASconfig
@22:58:17.41 AS.seek 1ml
@22:59:15.22 delay 1 second
@22:59:16.24 AFV.select :holdingCoil
@22:59:16.35 delay 1 second
@22:59:17.37 AS.reconfigure ASnorm
@22:59:17.42 AS.seek 0ml
@23:00:08.14 Setting up PCR for ACOPP5 -- writing /var/log/esp/ACOPP521apr1223h.pcr
@23:00:08.16 Building PCR Slug from ASV with MM3 mix, PP5 primer
@23:00:08.18 Priming with carrier
@23:00:08.20 ATV.dial ARV
@23:00:08.86 ARV.dial :carrier
@23:00:09.21 AS.seek 0.5ml
@23:00:34.78 AFV.select :carrierSolution
@23:00:34.91 delay 2 seconds
@23:00:36.93 AS.reconfigure ASconfig
@23:00:36.99 AS.seek 1ml
@23:01:06.03 delay 1 second
@23:01:07.06 AFV.select :holdingCoil
@23:01:07.16 delay 1 second
@23:01:08.18 ATV.dial :waste
@23:01:09.96 AS.reconfigure ASnorm
@23:01:10.02 AS.seek 0ml
@23:02:00.73 AFV.select :carrierSolution
@23:02:00.85 delay 2 seconds
@23:02:02.87 AS.reconfigure ASconfig
@23:02:02.93 AS.seek 0.5ml
@23:02:31.97 delay 1 second
@23:02:32.99 AFV.select :holdingCoil
@23:02:33.10 Building Slug
@23:02:33.11 ATV.dial :ARV
@23:02:34.84 delay 2 seconds
@23:02:36.87 ARV.dial :air1
@23:02:37.26 delay 2 seconds
@23:02:39.33 AS.reconfigure ASslow2
@23:02:39.39 AS.seek 0.506ml
@23:02:45.58 delay 2 seconds
@23:02:47.61 ARV.dial :MM3
@23:02:48.73 delay 2 seconds
@23:02:50.76 Adding MM3
@23:02:50.84 AS.seek 0.524ml
@23:03:09.48 delay 25 seconds
@23:03:34.51 ARV.dial :PP5
@23:03:34.92 Adding PP5
@23:03:34.94 delay 2 seconds
@23:03:37.01 AS.seek 0.53ml
@23:03:43.25 delay 10 seconds
@23:03:53.29 ARV.dial :air3
@23:03:53.69 adding ARV air
@23:03:53.71 delay 2 seconds
@23:03:55.78 AS.seek 0.536ml
@23:04:01.97 delay 5 seconds
@23:04:07.00 ARV.dial :carrier
@23:04:08.33 delay 7 seconds
@23:04:15.40 AS.seek 0.594ml
@23:05:14.00 delay 5 seconds
@23:05:19.03 ATV.dial :ASV
@23:05:19.44 delay 2 seconds
@23:05:21.51 AS.seek 0.6ml
@23:05:28.29 delay 4 seconds
@23:05:32.32 ATV.dial ARV
@23:05:32.91 delay 2 seconds
@23:05:34.94 ARV.dial :carrier
@23:05:34.95 delay 2 seconds
@23:05:37.02 AS.seek 0.65ml
@23:06:27.70 delay 4 seconds
@23:06:31.73 ATV.dial PCR
@23:06:32.37 ASV.dial :waste1
@23:06:32.39 ARV.dial :air1
@23:06:32.83 Positioning Slug by 0.324ml
@23:06:32.91 AS.seek 0.326ml
@23:11:58.84 delay 5 seconds
@23:12:03.87 ATV.dial ARV
@23:12:04.69 ASV.dial :PCRlock
@23:12:05.09 delay 5 seconds
@23:12:10.12 Running ACOPP5 ProfileIPc thermocycling
@23:12:10.13 <PCR> PCR.program ProfileIPc
@23:12:10.25 ProfileIPc should complete in < 1:26:35
@23:12:10.26 PCR.run 1:53:13.75
@00:20:24.52CEST13-Apr-21 PCR.off
@00:20:25.09 <MFBPCR> PCR for ACOPP5 completed -- closed /var/log/esp/ACOPP521apr1223h.pcr
@00:20:25.16 Cleaning PCR
@00:20:25.17 AFV.select :carrierSolution
@00:20:25.30 delay 2 seconds
@00:20:27.32 AS.reconfigure ASconfig
@00:20:27.37 AS.seek 0.5ml
@00:20:37.67 delay 1 second
@00:20:38.69 AFV.select :holdingCoil
@00:20:38.80 ASV.dial :waste1
@00:20:39.19 ATV.dial :air3
@00:20:40.54 AS.reconfigure ASslow1
@00:20:40.59 AS.seek 0.51ml
@00:20:42.90 delay 2 seconds
@00:20:44.93 ATV.dial :bleach
@00:20:45.58 AS.seek 0.81ml
@00:21:46.23 delay 3 seconds
@00:21:49.26 ATV.dial :air3
@00:21:49.84 delay 3 seconds
@00:21:52.91 AS.seek 0.835ml
@00:21:58.31 delay 3 seconds
@00:22:01.34 ATV.dial :bleach
@00:22:01.98 AS.seek 0.82ml
@00:22:05.27 delay 3 seconds
@00:22:08.30 ARV.dial :waste
@00:22:08.92 delay 3 seconds
@00:22:11.95 ATV.dial :ARV
@00:22:12.87 AS.reconfigure ASconfig
@00:22:12.92 AS.seek 0.47ml
@00:22:33.33 AFV.select :carrierSolution
@00:22:33.45 delay 2 seconds
@00:22:35.47 AS.seek 1ml
@00:23:06.23 delay 1 second
@00:23:07.25 AFV.select :holdingCoil
@00:23:07.36 delay 1 second
@00:23:08.38 AS.reconfigure ASfast
@00:23:08.44 AS.seek 0ml
@00:23:35.75 AFV.select :carrierSolution
@00:23:36.27 delay 2 seconds
@00:23:38.29 AS.reconfigure ASconfig
@00:23:38.34 AS.seek 1ml
@00:24:36.09 delay 1 second
@00:24:37.11 AFV.select :holdingCoil
@00:24:37.22 delay 1 second
@00:24:38.24 AS.reconfigure ASfast
@00:24:38.30 AS.seek 0ml
@00:25:05.62 AFV.select :carrierSolution
@00:25:05.74 delay 2 seconds
@00:25:07.76 AS.reconfigure ASconfig
@00:25:07.81 AS.seek 1ml
@00:26:05.56 delay 1 second
@00:26:06.58 AFV.select :holdingCoil
@00:26:06.69 delay 1 second
@00:26:07.71 AS.reconfigure ASfast
@00:26:07.77 AS.seek 0ml
@00:26:35.11 ARV.dial between carrier and air1
@00:26:35.65 ATV.dial :air3
@00:26:37.00 AS.reconfigure ASslow1
@00:26:37.06 AS.seek 0.01ml
@00:26:39.45 delay 2 seconds
@00:26:41.48 ATV.dial :bleach
@00:26:42.12 AS.seek 0.81ml
@00:29:23.27 delay 3 seconds
@00:29:26.30 ATV.dial :air3
@00:29:26.87 delay 3 seconds
@00:29:29.94 AS.seek 0.835ml
@00:29:35.22 delay 3 seconds
@00:29:38.25 ATV.dial :bleach
@00:29:38.89 AS.seek 0.82ml
@00:29:42.19 delay 3 seconds
@00:29:45.22 ATV.dial PCR
@00:29:45.63 AS.reconfigure ASconfig
@00:29:45.68 AS.seek 0ml
@00:30:33.08 PCR.program :bleachProfile
@00:30:33.16 PCR program should complete in < 2:45
@00:30:33.17 PCR.run 10:00
@00:33:28.96 PCR.off
@00:33:29.00 AFV.select :carrierSolution
@00:33:29.12 delay 2 seconds
@00:33:31.14 AS.seek 1ml
@00:34:28.92 delay 1 second
@00:34:29.94 AFV.select :holdingCoil
@00:34:30.05 delay 1 second
@00:34:31.07 AS.reconfigure ASfast
@00:34:31.13 AS.seek 0ml
@00:34:58.48 delay 3 seconds
@00:35:01.50 AFV.select :carrierSolution
@00:35:01.61 delay 2 seconds
@00:35:03.63 AS.reconfigure ASconfig
@00:35:03.69 AS.seek 1ml
@00:36:01.45 delay 1 second
@00:36:02.47 AFV.select :holdingCoil
@00:36:02.58 delay 1 second
@00:36:03.60 AS.reconfigure ASfast
@00:36:03.66 AS.seek 0ml
@00:36:31.00 delay 3 seconds
@00:36:34.02 AFV.select :carrierSolution
@00:36:34.13 delay 2 seconds
@00:36:36.15 AS.reconfigure ASconfig
@00:36:36.20 AS.seek 1ml
@00:37:33.98 delay 1 second
@00:37:35.00 AFV.select :holdingCoil
@00:37:35.53 delay 1 second
@00:37:36.55 AS.reconfigure ASfast
@00:37:36.61 AS.seek 0ml
@00:38:03.92 delay 3 seconds
@00:38:06.94 AFV.select :carrierSolution
@00:38:07.05 delay 2 seconds
@00:38:09.07 AS.reconfigure ASconfig
@00:38:09.12 AS.seek 1ml
@00:39:06.87 delay 1 second
@00:39:07.89 AFV.select :holdingCoil
@00:39:08.00 delay 1 second
@00:39:09.02 AS.reconfigure ASfast
@00:39:09.08 AS.seek 0ml
@00:39:36.41 delay 3 seconds
@00:39:39.43 AFV.select :carrierSolution
@00:39:39.54 delay 2 seconds
@00:39:41.56 AS.reconfigure ASconfig
@00:39:41.62 AS.seek 1ml
@00:40:39.36 delay 1 second
@00:40:40.38 AFV.select :holdingCoil
@00:40:40.49 delay 1 second
@00:40:41.51 AS.reconfigure ASnorm
@00:40:41.56 AS.seek 0ml
@00:41:32.28 Setting up PCR for AIPc -- writing /var/log/esp/AIPc21apr1300h.pcr
@00:41:32.30 Building PCR Slug from ASV with MM3 mix, PP6 primer
@00:41:32.32 Priming with carrier
@00:41:32.33 ATV.dial ARV
@00:41:32.98 ARV.dial :carrier
@00:41:33.33 AS.seek 0.5ml
@00:41:58.91 AFV.select :carrierSolution
@00:41:59.03 delay 2 seconds
@00:42:01.05 AS.reconfigure ASconfig
@00:42:01.12 AS.seek 1ml
@00:42:30.12 delay 1 second
@00:42:31.14 AFV.select :holdingCoil
@00:42:31.25 delay 1 second
@00:42:32.27 ATV.dial :waste
@00:42:34.04 AS.reconfigure ASnorm
@00:42:34.10 AS.seek 0ml
@00:43:24.78 AFV.select :carrierSolution
@00:43:24.91 delay 2 seconds
@00:43:26.93 AS.reconfigure ASconfig
@00:43:26.99 AS.seek 0.5ml
@00:43:56.05 delay 1 second
@00:43:57.07 AFV.select :holdingCoil
@00:43:57.18 Building Slug
@00:43:57.19 ATV.dial :ARV
@00:43:58.92 delay 2 seconds
@00:44:00.95 ARV.dial :air1
@00:44:01.35 delay 2 seconds
@00:44:03.42 AS.reconfigure ASslow2
@00:44:03.48 AS.seek 0.506ml
@00:44:09.80 delay 2 seconds
@00:44:11.83 ARV.dial :MM3
@00:44:12.92 delay 2 seconds
@00:44:14.95 Adding MM3
@00:44:15.04 AS.seek 0.524ml
@00:44:33.59 delay 25 seconds
@00:44:58.62 ARV.dial :PP6
@00:44:59.03 Adding PP6
@00:44:59.04 delay 2 seconds
@00:45:01.11 AS.seek 0.53ml
@00:45:07.28 delay 10 seconds
@00:45:17.32 ARV.dial :air1
@00:45:18.19 adding ARV air
@00:45:18.21 delay 2 seconds
@00:45:20.28 AS.seek 0.536ml
@00:45:26.47 delay 5 seconds
@00:45:31.50 ARV.dial :carrier
@00:45:31.91 delay 7 seconds
@00:45:38.99 AS.seek 0.594ml
@00:46:37.55 delay 5 seconds
@00:46:42.58 ATV.dial :ASV
@00:46:43.00 delay 2 seconds
@00:46:45.07 AS.seek 0.6ml
@00:46:51.23 delay 4 seconds
@00:46:55.26 ATV.dial ARV
@00:46:55.84 delay 2 seconds
@00:46:57.87 ARV.dial :carrier
@00:46:57.88 delay 2 seconds
@00:46:59.95 AS.seek 0.65ml
@00:47:50.72 delay 4 seconds
@00:47:54.75 ATV.dial PCR
@00:47:55.38 ASV.dial :waste1
@00:47:55.40 ARV.dial :air1
@00:47:55.83 Positioning Slug by 0.324ml
@00:47:55.91 AS.seek 0.326ml
@00:53:22.13 delay 5 seconds
@00:53:27.16 ATV.dial ARV
@00:53:28.00 ASV.dial :PCRlock
@00:53:28.41 delay 5 seconds
@00:53:33.44 Running AIPc ProfileIPc thermocycling
@00:53:33.45 <PCR> PCR.program ProfileIPc
@00:53:33.57 ProfileIPc should complete in < 1:26:35
@00:53:33.58 PCR.run 1:53:13.75
@02:01:49.31 PCR.off
@02:01:49.89 <MFBPCR> PCR for AIPc completed -- closed /var/log/esp/AIPc21apr1300h.pcr
@02:01:49.91 Full MFB Decontamination
@02:01:49.93 Decon extraction column and decon extSrc
@02:01:49.95 SPE.hold 2:46:40,55C
@02:01:50.00 ASV.dial :SPEdelivery
@02:01:50.67 delay 20 seconds
@02:02:10.70 AFV.select :carrierSolution
@02:02:10.81 delay 2 seconds
@02:02:12.83 AS.reconfigure ASconfig
@02:02:12.88 AS.seek 0.5ml
@02:02:23.21 delay 1 second
@02:02:24.23 AFV.select :holdingCoil
@02:02:24.34 ATV.dial :air1
@02:02:26.39 AS.reconfigure ASslow1
@02:02:26.45 AS.seek 0.52ml
@02:02:30.75 ATV.dial :ASV
@02:02:32.66 ASV.dial :SPErecovery
@02:02:33.34 AS.seek 0.62ml
@02:02:53.74 ATV.dial :waste
@02:02:55.93 AS.reconfigure ASnorm
@02:02:55.99 AS.seek 0ml
@02:03:27.56 deconEluateRecoveryLine extSrc
@02:03:27.57 ATV.dial :waste
@02:03:27.60 AS.seek 0ml
@02:03:27.69 ATV.dial :air3
@02:03:28.45 AS.reconfigure ASslow1
@02:03:28.51 AS.seek 0.01ml
@02:03:30.81 delay 2 seconds
@02:03:32.84 ATV.dial :bleach
@02:03:33.33 AS.seek 0.31ml
@02:04:34.33 delay 3 seconds
@02:04:37.36 ATV.dial :air3
@02:04:37.99 delay 3 seconds
@02:04:41.07 AS.seek 0.335ml
@02:04:46.37 delay 3 seconds
@02:04:49.40 ATV.dial :bleach
@02:04:50.09 AS.seek 0.32ml
@02:04:53.41 delay 3 seconds
@02:04:56.44 ATV.dial :extSrc
@02:04:57.12 AS.seek 0ml
@02:06:01.75 AFV.select :carrierSolution
@02:06:01.87 delay 2 seconds
@02:06:03.89 AS.reconfigure ASconfig
@02:06:03.95 AS.seek 0.5ml
@02:06:32.99 delay 1 second
@02:06:34.01 AFV.select :holdingCoil
@02:06:34.12 ATV.dial :extSrc
@02:06:34.14 AS.reconfigure ASslow1
@02:06:34.21 AS.seek 0ml
@02:08:15.03 AFV.select :carrierSolution
@02:08:15.15 delay 2 seconds
@02:08:17.17 AS.reconfigure ASconfig
@02:08:17.23 AS.seek 0.5ml
@02:08:46.27 delay 1 second
@02:08:47.29 AFV.select :holdingCoil
@02:08:47.40 ATV.dial :extSrc
@02:08:47.42 AS.reconfigure ASslow1
@02:08:47.48 AS.seek 0ml
@02:10:28.30 AFV.select :carrierSolution
@02:10:28.42 delay 2 seconds
@02:10:30.44 AS.reconfigure ASconfig
@02:10:30.50 AS.seek 0.5ml
@02:10:59.52 delay 1 second
@02:11:00.54 AFV.select :holdingCoil
@02:11:00.65 ATV.dial :extSrc
@02:11:00.67 AS.reconfigure ASslow1
@02:11:00.73 AS.seek 0ml
@02:12:41.55 AFV.select :carrierSolution
@02:12:41.67 delay 2 seconds
@02:12:43.69 AS.reconfigure ASconfig
@02:12:43.75 AS.seek 0.5ml
@02:13:12.82 delay 1 second
@02:13:13.84 AFV.select :holdingCoil
@02:13:13.95 ATV.dial :extSrc
@02:13:13.97 AS.reconfigure ASslow1
@02:13:14.03 AS.seek 0ml
@02:14:54.87 ATV.dial :air2
@02:14:56.25 AS.reconfigure ASslow0
@02:14:56.31 AS.seek 0.5ml
@02:15:46.86 ATV.dial :extSrc
@02:15:48.41 AS.seek 0.02ml
@02:16:37.00 delay 1 second
@02:16:38.03 ATV.dial :waste
@02:16:39.39 AS.reconfigure ASconfig
@02:16:39.44 AS.seek 0ml
@02:16:40.89 AFV.select :holdingCoil
@02:16:40.91 ATV.dial :waste
@02:16:40.94 AS.seek 0ml
@02:16:41.01 AFV.select :carrierSolution
@02:16:41.13 delay 2 seconds
@02:16:43.15 AS.seek 0.5ml
@02:17:12.17 delay 1 second
@02:17:13.19 AFV.select :holdingCoil
@02:17:13.30 ATV.dial :air3
@02:17:14.19 AS.reconfigure ASslow1
@02:17:14.25 AS.seek 0.52ml
@02:17:18.52 ATV.dial :air3
@02:17:18.61 AS.seek 0.53ml
@02:17:20.89 delay 2 seconds
@02:17:22.92 ATV.dial :bleach
@02:17:23.39 AS.seek 0.71ml
@02:17:59.85 delay 3 seconds
@02:18:02.88 ATV.dial :air3
@02:18:03.46 delay 3 seconds
@02:18:06.53 AS.seek 0.735ml
@02:18:11.87 delay 3 seconds
@02:18:14.90 ATV.dial :bleach
@02:18:15.55 AS.seek 0.72ml
@02:18:18.89 delay 3 seconds
@02:18:21.92 ATV.dial ASV
@02:18:23.00 ASV.dial :SPErecovery
@02:18:23.09 AS.seek 0.365ml
@02:19:34.74 ATV.dial :waste
@02:19:36.75 AS.reconfigure ASnorm
@02:19:36.80 AS.seek 0ml
@02:19:55.53 ATV.dial :air3
@02:19:56.41 AS.reconfigure ASslow1
@02:19:56.47 AS.seek 0.01ml
@02:19:58.83 delay 2 seconds
@02:20:00.86 ATV.dial :bleach
@02:20:01.36 AS.seek 0.58ml
@02:21:56.27 delay 3 seconds
@02:21:59.30 ATV.dial :air3
@02:21:59.88 delay 3 seconds
@02:22:02.95 AS.seek 0.605ml
@02:22:08.25 delay 3 seconds
@02:22:11.28 ATV.dial :bleach
@02:22:11.92 AS.seek 0.59ml
@02:22:15.27 delay 3 seconds
@02:22:18.30 ATV.dial :ASV
@02:22:19.39 ASV.dial :wasteR
@02:22:19.79 AS.seek 0ml
@02:24:18.80 delay 1:00
@02:25:18.84 ATV.dial ASV
@02:25:18.85 AFV.select :carrierSolution
@02:25:18.97 delay 2 seconds
@02:25:20.99 AS.reconfigure ASconfig
@02:25:21.04 AS.seek 1ml
@02:26:18.77 delay 1 second
@02:26:19.79 AFV.select :holdingCoil
@02:26:20.34 delay 1 second
@02:26:21.36 ASV.dial :wasteR
@02:26:21.37 AS.reconfigure ASslow1
@02:26:21.45 AS.seek 0ml
@02:29:42.85 delay 10 seconds
@02:29:52.89 ATV.dial :air3
@02:29:54.48 AS.seek 0.01ml
@02:29:56.80 delay 2 seconds
@02:29:58.83 ATV.dial :bleach
@02:29:59.47 AS.seek 0.58ml
@02:31:54.38 delay 3 seconds
@02:31:57.41 ATV.dial :air3
@02:31:57.99 delay 3 seconds
@02:32:01.07 AS.seek 0.605ml
@02:32:06.50 delay 3 seconds
@02:32:09.53 ATV.dial :bleach
@02:32:10.19 AS.seek 0.59ml
@02:32:13.46 delay 3 seconds
@02:32:16.49 ATV.dial ASV
@02:32:17.57 ASV.dial :SPEdelivery
@02:32:17.97 AS.seek 0ml
@02:34:17.03 delay 1:00
@02:35:17.06 AFV.select :carrierSolution
@02:35:17.17 delay 2 seconds
@02:35:19.19 AS.reconfigure ASconfig
@02:35:19.25 AS.seek 1ml
@02:36:17.03 delay 1 second
@02:36:18.05 AFV.select :holdingCoil
@02:36:18.16 delay 1 second
@02:36:19.18 ATV.dial ASV
@02:36:19.19 ASV.dial :SPEdelivery
@02:36:19.21 AS.reconfigure ASslow1
@02:36:19.28 AS.seek 0ml
@02:39:40.66 delay 10 seconds
@02:39:50.70 ATV.dial :air2
@02:39:51.58 AS.reconfigure ASslow0
@02:39:51.64 AS.seek 0.8ml
@02:41:12.44 delay 3 seconds
@02:41:15.47 ATV.dial :ASV
@02:41:16.50 ASV.dial :SPEdelivery
@02:41:16.52 AS.reconfigure ASslow1
@02:41:16.59 AS.seek 0.03ml
@02:43:51.67 delay 20 seconds
@02:44:11.71 ATV.dial :waste,via: :air3
@02:44:13.53 AS.reconfigure ASconfig
@02:44:13.58 AS.seek 0ml
@02:44:15.61 SPE.coast
@02:44:15.69 Removing Bleach from Mixing Coil
@02:44:15.70 AFV.select :carrierSolution
@02:44:15.82 delay 2 seconds
@02:44:17.84 AS.seek 0.5ml
@02:44:46.86 delay 1 second
@02:44:47.88 AFV.select :holdingCoil
@02:44:47.99 ATV.dial :ASV
@02:44:49.75 ASV.dial :SPErecovery
@02:44:50.42 AS.reconfigure ASslow1
@02:44:50.48 AS.seek 0.73ml
@02:45:37.05 ASV.dial :wasteD
@02:45:37.75 AS.reconfigure ASnorm
@02:45:37.80 AS.seek 0ml
@02:46:14.91 AFV.select :carrierSolution
@02:46:15.03 delay 2 seconds
@02:46:17.05 AS.reconfigure ASconfig
@02:46:17.11 AS.seek 0.5ml
@02:46:46.14 delay 1 second
@02:46:47.16 AFV.select :holdingCoil
@02:46:47.27 ATV.dial ASV
@02:46:47.28 ASV.dial :SPErecovery
@02:46:47.94 AS.reconfigure ASslow0
@02:46:48.00 AS.seek 0.3ml
@02:47:08.41 ATV.dial :air2
@02:47:09.50 AS.reconfigure ASslow1
@02:47:09.56 AS.seek 0.32ml
@02:47:13.92 ATV.dial ASV
@02:47:15.00 AS.reconfigure ASslow0
@02:47:15.06 AS.seek 0.57ml
@02:47:40.53 ASV.dial :wasteD
@02:47:41.14 AS.reconfigure ASnorm
@02:47:41.20 AS.seek 0ml
@02:48:10.27 AFV.select :carrierSolution
@02:48:10.39 delay 2 seconds
@02:48:12.41 AS.reconfigure ASconfig
@02:48:12.47 AS.seek 0.5ml
@02:48:41.53 delay 1 second
@02:48:42.55 AFV.select :holdingCoil
@02:48:42.66 ATV.dial ASV
@02:48:42.67 ASV.dial :SPErecovery
@02:48:43.34 AS.reconfigure ASslow0
@02:48:43.40 AS.seek 0.3ml
@02:49:03.82 ATV.dial :air2
@02:49:04.91 AS.reconfigure ASslow1
@02:49:04.97 AS.seek 0.32ml
@02:49:09.28 ATV.dial ASV
@02:49:10.38 AS.reconfigure ASslow0
@02:49:10.44 AS.seek 0.57ml
@02:49:35.82 ASV.dial :wasteD
@02:49:36.44 AS.reconfigure ASnorm
@02:49:36.50 AS.seek 0ml
@02:50:05.55 ATV.dial :air2
@02:50:06.59 AS.reconfigure ASslow0
@02:50:06.64 AS.seek 0.3ml
@02:50:37.22 ATV.dial :ASV
@02:50:38.25 ASV.dial :wasteD
@02:50:38.27 AS.reconfigure ASconfig
@02:50:38.34 AS.seek 0.05ml
@02:50:53.03 ATV.dial :waste
@02:50:55.00 AS.seek 0ml
@02:50:58.61 ASV.dial :SPEdelivery
@02:50:59.70 Cleaning PCR
@02:50:59.71 AFV.select :carrierSolution
@02:50:59.83 delay 2 seconds
@02:51:01.85 AS.seek 0.5ml
@02:51:30.88 delay 1 second
@02:51:31.90 AFV.select :holdingCoil
@02:51:32.01 ASV.dial :waste1
@02:51:32.38 ATV.dial :air3
@02:51:33.09 AS.reconfigure ASslow1
@02:51:33.15 AS.seek 0.51ml
@02:51:35.41 delay 2 seconds
@02:51:37.44 ATV.dial :bleach
@02:51:37.91 AS.seek 0.81ml
@02:52:38.50 delay 3 seconds
@02:52:41.53 ATV.dial :air3
@02:52:42.11 delay 3 seconds
@02:52:45.18 AS.seek 0.835ml
@02:52:50.47 delay 3 seconds
@02:52:53.50 ATV.dial :bleach
@02:52:54.13 AS.seek 0.82ml
@02:52:57.44 delay 3 seconds
@02:53:00.47 ARV.dial :waste
@02:53:01.10 delay 3 seconds
@02:53:04.13 ATV.dial :ARV
@02:53:05.05 AS.reconfigure ASconfig
@02:53:05.11 AS.seek 0.47ml
@02:53:25.50 AFV.select :carrierSolution
@02:53:25.62 delay 2 seconds
@02:53:27.64 AS.seek 1ml
@02:53:58.43 delay 1 second
@02:53:59.45 AFV.select :holdingCoil
@02:53:59.56 delay 1 second
@02:54:00.58 AS.reconfigure ASfast
@02:54:00.64 AS.seek 0ml
@02:54:27.99 AFV.select :carrierSolution
@02:54:28.11 delay 2 seconds
@02:54:30.13 AS.reconfigure ASconfig
@02:54:30.18 AS.seek 1ml
@02:55:27.96 delay 1 second
@02:55:28.99 AFV.select :holdingCoil
@02:55:29.10 delay 1 second
@02:55:30.12 AS.reconfigure ASfast
@02:55:30.18 AS.seek 0ml
@02:55:57.52 AFV.select :carrierSolution
@02:55:57.64 delay 2 seconds
@02:55:59.66 AS.reconfigure ASconfig
@02:55:59.72 AS.seek 1ml
@02:56:57.46 delay 1 second
@02:56:58.48 AFV.select :holdingCoil
@02:56:58.59 delay 1 second
@02:56:59.61 AS.reconfigure ASfast
@02:56:59.67 AS.seek 0ml
@02:57:27.02 ARV.dial between carrier and air1
@02:57:27.56 ATV.dial :air3
@02:57:28.91 AS.reconfigure ASslow1
@02:57:28.97 AS.seek 0.01ml
@02:57:31.36 delay 2 seconds
@02:57:33.39 ATV.dial :bleach
@02:57:34.03 AS.seek 0.81ml
@03:00:15.22 delay 3 seconds
@03:00:18.25 ATV.dial :air3
@03:00:18.83 delay 3 seconds
@03:00:21.90 AS.seek 0.835ml
@03:00:27.27 delay 3 seconds
@03:00:30.30 ATV.dial :bleach
@03:00:30.94 AS.seek 0.82ml
@03:00:34.22 delay 3 seconds
@03:00:37.25 ATV.dial PCR
@03:00:37.67 AS.reconfigure ASconfig
@03:00:37.72 AS.seek 0ml
@03:01:25.16 PCR.program :bleachProfile
@03:01:25.23 PCR program should complete in < 2:45
@03:01:25.24 PCR.run 10:00
@03:04:27.50 PCR.off
@03:04:27.55 AFV.select :carrierSolution
@03:04:27.66 delay 2 seconds
@03:04:29.68 AS.seek 1ml
@03:05:27.47 delay 1 second
@03:05:28.50 AFV.select :holdingCoil
@03:05:28.61 delay 1 second
@03:05:29.63 AS.reconfigure ASfast
@03:05:29.69 AS.seek 0ml
@03:05:57.03 delay 3 seconds
@03:06:00.05 AFV.select :carrierSolution
@03:06:00.16 delay 2 seconds
@03:06:02.18 AS.reconfigure ASconfig
@03:06:02.24 AS.seek 1ml
@03:06:59.99 delay 1 second
@03:07:01.01 AFV.select :holdingCoil
@03:07:01.12 delay 1 second
@03:07:02.14 AS.reconfigure ASfast
@03:07:02.20 AS.seek 0ml
@03:07:29.53 delay 3 seconds
@03:07:32.55 AFV.select :carrierSolution
@03:07:32.66 delay 2 seconds
@03:07:34.68 AS.reconfigure ASconfig
@03:07:34.74 AS.seek 1ml
@03:08:32.50 delay 1 second
@03:08:33.52 AFV.select :holdingCoil
@03:08:33.63 delay 1 second
@03:08:34.65 AS.reconfigure ASfast
@03:08:34.71 AS.seek 0ml
@03:09:02.04 delay 3 seconds
@03:09:05.06 AFV.select :carrierSolution
@03:09:05.17 delay 2 seconds
@03:09:07.19 AS.reconfigure ASconfig
@03:09:07.26 AS.seek 1ml
@03:10:05.07 delay 1 second
@03:10:06.09 AFV.select :holdingCoil
@03:10:06.20 delay 1 second
@03:10:07.22 AS.reconfigure ASfast
@03:10:07.27 AS.seek 0ml
@03:10:34.61 delay 3 seconds
@03:10:37.63 AFV.select :carrierSolution
@03:10:37.74 delay 2 seconds
@03:10:39.76 AS.reconfigure ASconfig
@03:10:39.82 AS.seek 1ml
@03:11:37.58 delay 1 second
@03:11:38.60 AFV.select :holdingCoil
@03:11:38.71 delay 1 second
@03:11:39.73 AS.reconfigure ASnorm
@03:11:39.78 AS.seek 0ml
@03:12:30.52 AFV.select :carrierSolution
@03:12:30.64 delay 2 seconds
@03:12:32.66 AS.reconfigure ASconfig
@03:12:32.71 AS.seek 1ml
@03:13:30.49 delay 1 second
@03:13:31.51 AFV.select :holdingCoil
@03:13:31.62 delay 1 second
@03:13:32.64 ATV.dial :waste
@03:13:33.92 AS.reconfigure ASnorm
@03:13:33.97 AS.seek 0.5ml
@03:13:59.52 ATV.dial :waste2
@03:14:00.86 AS.seek 0ml
@03:14:26.42 <bspe> Session Ended
@21:06:28.68CEST13-Apr-21 <MAIN> ESP2 v3.30 I2C Gateway v3.06 for core bus
@21:06:31.23 
Configuration for mer 7/20/20 brent@mbari.org
/home/esp/esp2/type/shallow/mer/configure.rb
@21:06:31.32 
Configuration for MFB3 by preston@mbari.org
/home/esp/esp2/type/mfb/MFB3/configure.rb
@21:06:36.08 Polling Can every 10:00
@21:06:37.79 CS.configure :default
@21:06:37.83 CS.hold
@21:06:37.87 CS.configure :default
@21:06:38.01 PS.configure :default
@21:06:38.18 SS.configure :default
@21:06:38.26 SS.hold
@21:06:38.35 SS.configure :default
@21:06:38.52 CC.configure :default
@21:06:38.59 CC.hold
@21:06:38.68 CC.configure :default
@21:06:38.80 PC.configure :default
@21:06:38.84 PC.hold
@21:06:38.88 PC.configure :default
@21:06:38.98 Forearm.configure :loose
@21:06:39.02 Forearm.hold
@21:06:39.07 Forearm.configure :loose
@21:06:39.15 Elbow.configure :loose
@21:06:39.19 Elbow.hold
@21:06:39.24 Elbow.configure :loose
@21:06:39.34 SC.configure :loose
@21:06:39.38 SC.hold
@21:06:39.42 SC.configure :loose
@21:06:39.50 SE.configure :default
@21:06:39.54 SE.hold
@21:06:39.60 SE.configure :default
@21:06:39.64 CH.coast
@21:06:39.69 CH.configure :default
@21:06:39.72 PH.coast
@21:06:39.77 PH.configure :default
@21:06:39.87 SPE.coast
@21:06:39.91 SPE.configure SPEconfig
@21:06:39.99 AS.configure ASconfig
@21:06:40.03 AS.hold
@21:06:40.08 AS.configure ASconfig
@21:06:40.12 PCR.off
@21:06:40.16 PCR.pidtemp= 50,0.1,0,300,25,25
@21:06:40.19 PCR.caltemp= 2.5051,0.0372,0
@21:06:40.22 All dwarf controllers running firmware version 3.75
@21:06:40.43 Cleanly exited at 13:10:53CEST13-Apr-21
@21:06:40.46 Loading /home/esp/esp2/mission/SYBRIPc.rb mission
@21:06:40.73 <MFBPCR> Setting up PCR for SYBRIPc -- writing /var/log/esp/SYBRIPc21apr1321h.pcr
@21:06:40.78 Building PCR Slug from elutionWater with MM1 mix, PP6 primer
@21:06:40.81 Priming with carrier
@21:06:40.83 ATV.dial ARV
@21:06:40.94 ARV.dial :carrier
@21:06:41.28 <bkha> Session Begun
@21:06:41.50 <MFBPCR> AS.reconfigure ASnorm
@21:06:41.60 AS.seek 0.826ml
@21:06:46.30 <bkha> -> showlog 00
@21:06:51.17 -> showlog 100
@21:07:07.23 <MFBPCR> AFV.select :carrierSolution
@21:07:07.36 delay 2 seconds
@21:07:09.38 AS.reconfigure ASconfig
@21:07:09.45 AS.seek 1ml
@21:07:19.72 delay 1 second
@21:07:20.74 AFV.select :holdingCoil
@21:07:20.85 delay 1 second
@21:07:21.87 ATV.dial :waste
@21:07:23.63 AS.reconfigure ASnorm
@21:07:23.69 AS.seek 0ml
@21:08:14.44 AFV.select :carrierSolution
@21:08:14.57 delay 2 seconds
@21:08:16.59 AS.reconfigure ASconfig
@21:08:16.65 AS.seek 0.5ml
@21:08:45.70 delay 1 second
@21:08:46.72 AFV.select :holdingCoil
@21:08:46.83 Building Slug
@21:08:46.84 ATV.dial :ARV
@21:08:48.58 delay 2 seconds
@21:08:50.61 ARV.dial :air1
@21:08:51.02 delay 2 seconds
@21:08:53.09 AS.reconfigure ASslow2
@21:08:53.16 AS.seek 0.506ml
@21:08:59.34 delay 2 seconds
@21:09:01.37 ARV.dial :MM1
@21:09:02.01 delay 2 seconds
@21:09:04.04 Adding MM1
@21:09:04.12 AS.seek 0.524ml
@21:09:22.34 delay 25 seconds
@21:09:47.69 ARV.dial :PP6
@21:09:49.10 Adding PP6
@21:09:49.11 delay 2 seconds
@21:09:51.18 AS.seek 0.53ml
@21:09:57.48 delay 10 seconds
@21:10:07.52 ARV.dial :air1
@21:10:08.40 adding ARV air
@21:10:08.41 delay 2 seconds
@21:10:10.48 AS.seek 0.536ml
@21:10:16.76 delay 5 seconds
@21:10:21.79 ARV.dial :carrier
@21:10:22.20 delay 7 seconds
@21:10:29.29 AS.seek 0.594ml
@21:11:27.80 delay 5 seconds
@21:11:32.83 ATV.dial :elutionWater
@21:11:34.34 delay 2 seconds
@21:11:36.41 AS.seek 0.6ml
@21:11:42.83 delay 4 seconds
@21:11:46.86 ATV.dial ARV
@21:11:48.36 delay 2 seconds
@21:11:50.39 ARV.dial :carrier
@21:11:50.40 delay 2 seconds
@21:11:52.47 AS.seek 0.65ml
@21:12:43.23 delay 4 seconds
@21:12:47.26 ATV.dial PCR
@21:12:48.06 ASV.dial :waste1
@21:12:48.49 ARV.dial :air1
@21:12:48.90 Positioning Slug by 0.324ml
@21:12:48.98 AS.seek 0.326ml
@21:18:15.11 delay 5 seconds
@21:18:20.14 ATV.dial ARV
@21:18:20.94 ASV.dial :PCRlock
@21:18:21.34 delay 5 seconds
@21:18:26.37 Running SYBRIPc Profile60CIQ1 thermocycling
@21:18:26.38 <PCR> PCR.program Profile60CIQ1
@21:18:26.51 Profile60CIQ1 should complete in < 1:56:15
@21:18:26.52 PCR.run 2:30:18.75
@22:29:22.83 <bkha> -> PCR.sample
@22:31:41.59 -> PCR.sample
@22:53:26.98 -> PCR.sample
@22:56:00.49 <bkha#2> Session Begun
@22:56:00.79 <bkha> Session Ended
@22:56:16.99 <bkha#2> -> showlog 10
@22:56:25.60 -> PCR.sample
@22:57:21.11 <PCR> PCR.off
@22:57:21.68 <MFBPCR> PCR for SYBRIPc completed -- closed /var/log/esp/SYBRIPc21apr1321h.pcr
@22:57:21.76 Cleaning PCR
@22:57:21.77 AFV.select :carrierSolution
@22:57:21.89 delay 2 seconds
@22:57:23.91 AS.reconfigure ASconfig
@22:57:23.97 AS.seek 0.5ml
@22:57:34.27 delay 1 second
@22:57:35.29 AFV.select :holdingCoil
@22:57:35.40 ASV.dial :waste1
@22:57:35.80 ATV.dial :air3
@22:57:37.15 AS.reconfigure ASslow1
@22:57:37.21 AS.seek 0.51ml
@22:57:39.48 delay 2 seconds
@22:57:41.51 ATV.dial :bleach
@22:57:42.15 AS.seek 0.81ml
@22:58:42.73 delay 3 seconds
@22:58:45.76 ATV.dial :air3
@22:58:46.34 delay 3 seconds
@22:58:49.41 AS.seek 0.835ml
@22:58:54.73 delay 3 seconds
@22:58:57.76 ATV.dial :bleach
@22:58:58.40 AS.seek 0.82ml
@22:59:01.72 delay 3 seconds
@22:59:04.75 ARV.dial :waste
@22:59:05.37 delay 3 seconds
@22:59:08.40 ATV.dial :ARV
@22:59:09.33 AS.reconfigure ASconfig
@22:59:09.38 AS.seek 0.47ml
@22:59:29.78 AFV.select :carrierSolution
@22:59:29.90 delay 2 seconds
@22:59:31.92 AS.seek 1ml
@23:00:02.69 delay 1 second
@23:00:03.71 AFV.select :holdingCoil
@23:00:03.82 delay 1 second
@23:00:04.84 AS.reconfigure ASfast
@23:00:04.89 AS.seek 0ml
@23:00:32.23 AFV.select :carrierSolution
@23:00:32.35 delay 2 seconds
@23:00:34.37 AS.reconfigure ASconfig
@23:00:34.42 AS.seek 1ml
@23:01:32.17 delay 1 second
@23:01:33.19 AFV.select :holdingCoil
@23:01:33.30 delay 1 second
@23:01:34.32 AS.reconfigure ASfast
@23:01:34.37 AS.seek 0ml
@23:02:01.72 AFV.select :carrierSolution
@23:02:01.84 delay 2 seconds
@23:02:03.87 AS.reconfigure ASconfig
@23:02:03.92 AS.seek 1ml
@23:03:01.69 delay 1 second
@23:03:02.71 AFV.select :holdingCoil
@23:03:02.82 delay 1 second
@23:03:03.84 AS.reconfigure ASfast
@23:03:03.90 AS.seek 0ml
@23:03:31.23 ARV.dial between carrier and air1
@23:03:31.76 ATV.dial :air3
@23:03:33.11 AS.reconfigure ASslow1
@23:03:33.17 AS.seek 0.01ml
@23:03:35.48 delay 2 seconds
@23:03:37.51 ATV.dial :bleach
@23:03:38.27 AS.seek 0.81ml
@23:06:19.44 delay 3 seconds
@23:06:22.47 ATV.dial :air3
@23:06:23.05 delay 3 seconds
@23:06:26.12 AS.seek 0.835ml
@23:06:31.51 delay 3 seconds
@23:06:34.54 ATV.dial :bleach
@23:06:35.18 AS.seek 0.82ml
@23:06:38.61 delay 3 seconds
@23:06:41.64 ATV.dial PCR
@23:06:42.07 AS.reconfigure ASconfig
@23:06:42.12 AS.seek 0ml
@23:06:55.68 <bkha#2> Session Ended
@23:07:29.55 <MFBPCR> PCR.program :bleachProfile
@23:07:29.61 PCR program should complete in < 2:45
@23:07:29.62 PCR.run 10:00
@23:07:43.36 <bkha> Session Begun
@23:07:49.68 -> showlog 10
@23:10:25.78 <MFBPCR> PCR.off
@23:10:25.82 AFV.select :carrierSolution
@23:10:25.94 delay 2 seconds
@23:10:27.96 AS.seek 1ml
@23:11:25.75 delay 1 second
@23:11:26.77 AFV.select :holdingCoil
@23:11:26.88 delay 1 second
@23:11:27.90 AS.reconfigure ASfast
@23:11:27.97 AS.seek 0ml
@23:11:55.33 delay 3 seconds
@23:11:58.35 AFV.select :carrierSolution
@23:11:58.46 delay 2 seconds
@23:12:00.48 AS.reconfigure ASconfig
@23:12:00.55 AS.seek 1ml
@23:12:58.33 delay 1 second
@23:12:59.35 AFV.select :holdingCoil
@23:12:59.46 delay 1 second
@23:13:00.48 AS.reconfigure ASfast
@23:13:00.53 AS.seek 0ml
@23:13:27.89 delay 3 seconds
@23:13:30.91 AFV.select :carrierSolution
@23:13:31.02 delay 2 seconds
@23:13:33.04 AS.reconfigure ASconfig
@23:13:33.10 AS.seek 1ml
@23:14:30.86 delay 1 second
@23:14:31.88 AFV.select :holdingCoil
@23:14:31.99 delay 1 second
@23:14:33.01 AS.reconfigure ASfast
@23:14:33.07 AS.seek 0ml
@23:15:00.41 delay 3 seconds
@23:15:03.43 AFV.select :carrierSolution
@23:15:03.54 delay 2 seconds
@23:15:05.56 AS.reconfigure ASconfig
@23:15:05.63 AS.seek 1ml
@23:16:03.39 delay 1 second
@23:16:04.41 AFV.select :holdingCoil
@23:16:04.52 delay 1 second
@23:16:05.54 AS.reconfigure ASfast
@23:16:05.60 AS.seek 0ml
@23:16:32.92 delay 3 seconds
@23:16:35.94 AFV.select :carrierSolution
@23:16:36.05 delay 2 seconds
@23:16:38.07 AS.reconfigure ASconfig
@23:16:38.14 AS.seek 1ml
@23:17:35.92 delay 1 second
@23:17:36.94 AFV.select :holdingCoil
@23:17:37.06 delay 1 second
@23:17:38.08 AS.reconfigure ASnorm
@23:17:38.14 AS.seek 0ml
@23:18:28.87 <bkha> Session Ended
@23:19:04.73CEST13-Apr-21 <MAIN> ESP2 v3.30 I2C Gateway v3.06 for core bus
@23:19:07.27 
Configuration for mer 7/20/20 brent@mbari.org
/home/esp/esp2/type/shallow/mer/configure.rb
@23:19:07.36 
Configuration for MFB3 by preston@mbari.org
/home/esp/esp2/type/mfb/MFB3/configure.rb
@23:19:12.54 Polling Can every 10:00
@23:19:14.25 CS.configure :default
@23:19:14.29 CS.hold
@23:19:14.36 CS.configure :default
@23:19:14.44 PS.configure :default
@23:19:14.52 SS.configure :default
@23:19:14.56 SS.hold
@23:19:14.61 SS.configure :default
@23:19:14.69 CC.configure :default
@23:19:14.73 CC.hold
@23:19:14.79 CC.configure :default
@23:19:14.87 PC.configure :default
@23:19:14.91 PC.hold
@23:19:14.96 PC.configure :default
@23:19:15.06 Forearm.configure :loose
@23:19:15.10 Forearm.hold
@23:19:15.14 Forearm.configure :loose
@23:19:15.22 Elbow.configure :loose
@23:19:15.26 Elbow.hold
@23:19:15.30 Elbow.configure :loose
@23:19:15.40 SC.configure :loose
@23:19:15.44 SC.hold
@23:19:15.49 SC.configure :loose
@23:19:15.57 SE.configure :default
@23:19:15.60 SE.hold
@23:19:15.65 SE.configure :default
@23:19:15.68 CH.coast
@23:19:15.73 CH.configure :default
@23:19:15.75 PH.coast
@23:19:15.80 PH.configure :default
@23:19:15.90 SPE.coast
@23:19:15.94 SPE.configure SPEconfig
@23:19:16.02 AS.configure ASconfig
@23:19:16.05 AS.hold
@23:19:16.11 AS.configure ASconfig
@23:19:16.16 PCR.off
@23:19:16.19 PCR.pidtemp= 50,0.1,0,300,25,25
@23:19:16.22 PCR.caltemp= 2.5051,0.0372,0
@23:19:16.25 All dwarf controllers running firmware version 3.75
@23:19:16.46 Cleanly exited at 23:18:28CEST13-Apr-21
@23:19:16.49 Loading /home/esp/esp2/mission/SYBRIPc.rb mission
@23:19:16.54 Cleaning PCR
@23:19:16.55 AFV.select :carrierSolution
@23:19:16.65 delay 2 seconds
@23:19:18.67 AS.seek 0.5ml
@23:19:19.29 <bkha> Session Begun
@23:19:23.20 -> showlog 100
@23:19:33.59 -> exit
@23:19:33.60 Session Ended
@23:19:47.73 <MAIN> delay 1 second
@23:19:48.75 AFV.select :holdingCoil
@23:19:48.86 ASV.dial :waste1
@23:19:48.92 ATV.dial :air3
@23:19:49.79 AS.reconfigure ASslow1
@23:19:49.83 AS.seek 0.51ml
@23:19:52.17 delay 2 seconds
@23:19:54.20 ATV.dial :bleach
@23:19:54.83 AS.seek 0.81ml
@23:20:55.44 delay 3 seconds
@23:20:58.47 ATV.dial :air3
@23:20:59.03 delay 3 seconds
@23:21:02.10 AS.seek 0.835ml
@23:21:07.38 delay 3 seconds
@23:21:10.41 ATV.dial :bleach
@23:21:11.03 AS.seek 0.82ml
@23:21:14.42 delay 3 seconds
@23:21:17.45 ARV.dial :waste
@23:21:17.95 delay 3 seconds
@23:21:20.98 ATV.dial :ARV
@23:21:21.90 AS.reconfigure ASconfig
@23:21:21.94 AS.seek 0.47ml
@23:21:42.34 AFV.select :carrierSolution
@23:21:42.45 delay 2 seconds
@23:21:44.47 AS.seek 1ml
@23:22:15.20 delay 1 second
@23:22:16.22 AFV.select :holdingCoil
@23:22:16.33 delay 1 second
@23:22:17.35 AS.reconfigure ASfast
@23:22:17.39 AS.seek 0ml
@23:22:44.72 AFV.select :carrierSolution
@23:22:44.83 delay 2 seconds
@23:22:46.85 AS.reconfigure ASconfig
@23:22:46.90 AS.seek 1ml
@23:23:44.67 delay 1 second
@23:23:45.69 AFV.select :holdingCoil
@23:23:45.80 delay 1 second
@23:23:46.82 AS.reconfigure ASfast
@23:23:46.87 AS.seek 0ml
@23:24:14.19 AFV.select :carrierSolution
@23:24:14.30 delay 2 seconds
@23:24:16.32 AS.reconfigure ASconfig
@23:24:16.73 AS.seek 1ml
@23:25:14.50 delay 1 second
@23:25:15.51 AFV.select :holdingCoil
@23:25:15.62 delay 1 second
@23:25:16.64 AS.reconfigure ASfast
@23:25:16.69 AS.seek 0ml
@23:25:44.03 ARV.dial between carrier and air1
@23:25:44.56 ATV.dial :air3
@23:25:45.90 AS.reconfigure ASslow1
@23:25:45.94 AS.seek 0.01ml
@23:25:48.33 delay 2 seconds
@23:25:50.36 ATV.dial :bleach
@23:25:50.98 AS.seek 0.81ml
@23:28:32.09 delay 3 seconds
@23:28:35.12 ATV.dial :air3
@23:28:35.69 delay 3 seconds
@23:28:38.76 AS.seek 0.835ml
@23:28:44.09 delay 3 seconds
@23:28:47.12 ATV.dial :bleach
@23:28:47.75 AS.seek 0.82ml
@23:28:51.02 delay 3 seconds
@23:28:54.05 ATV.dial PCR
@23:28:54.46 AS.reconfigure ASconfig
@23:28:54.50 AS.seek 0ml
@23:29:41.86 PCR.program :bleachProfile
@23:29:41.92 PCR program should complete in < 2:45
@23:29:41.93 PCR.run 10:00
@23:32:40.75 PCR.off
@23:32:40.77 AFV.select :carrierSolution
@23:32:40.88 delay 2 seconds
@23:32:42.90 AS.seek 1ml
@23:33:40.62 delay 1 second
@23:33:41.63 AFV.select :holdingCoil
@23:33:41.74 delay 1 second
@23:33:42.76 AS.reconfigure ASfast
@23:33:42.81 AS.seek 0ml
@23:34:10.14 delay 3 seconds
@23:34:13.16 AFV.select :carrierSolution
@23:34:13.27 delay 2 seconds
@23:34:15.29 AS.reconfigure ASconfig
@23:34:15.34 AS.seek 1ml
@23:35:13.08 delay 1 second
@23:35:14.09 AFV.select :holdingCoil
@23:35:14.20 delay 1 second
@23:35:15.22 AS.reconfigure ASfast
@23:35:15.27 AS.seek 0ml
@23:35:42.59 delay 3 seconds
@23:35:45.61 AFV.select :carrierSolution
@23:35:45.72 delay 2 seconds
@23:35:47.74 AS.reconfigure ASconfig
@23:35:47.79 AS.seek 1ml
@23:36:45.56 delay 1 second
@23:36:46.57 AFV.select :holdingCoil
@23:36:46.68 delay 1 second
@23:36:47.70 AS.reconfigure ASfast
@23:36:47.75 AS.seek 0ml
@23:37:15.06 delay 3 seconds
@23:37:18.08 AFV.select :carrierSolution
@23:37:18.19 delay 2 seconds
@23:37:20.21 AS.reconfigure ASconfig
@23:37:20.26 AS.seek 1ml
@23:38:17.98 delay 1 second
@23:38:18.99 AFV.select :holdingCoil
@23:38:19.10 delay 1 second
@23:38:20.12 AS.reconfigure ASfast
@23:38:20.17 AS.seek 0ml
@23:38:47.48 delay 3 seconds
@23:38:50.50 AFV.select :carrierSolution
@23:38:50.61 delay 2 seconds
@23:38:52.63 AS.reconfigure ASconfig
@23:38:52.69 AS.seek 1ml
@23:39:50.41 delay 1 second
@23:39:51.42 AFV.select :holdingCoil
@23:39:51.53 delay 1 second
@23:39:52.55 AS.reconfigure ASnorm
@23:39:52.60 AS.seek 0ml
@23:40:43.33 Cleaning PCR
@23:40:43.34 AFV.select :carrierSolution
@23:40:43.44 delay 2 seconds
@23:40:45.46 AS.reconfigure ASconfig
@23:40:45.51 AS.seek 0.5ml
@23:41:14.55 delay 1 second
@23:41:15.57 AFV.select :holdingCoil
@23:41:15.68 ASV.dial :waste1
@23:41:15.69 ATV.dial :air3
@23:41:16.56 AS.reconfigure ASslow1
@23:41:16.60 AS.seek 0.51ml
@23:41:18.95 delay 2 seconds
@23:41:20.98 ATV.dial :bleach
@23:41:21.61 AS.seek 0.81ml
@23:42:22.19 delay 3 seconds
@23:42:25.22 ATV.dial :air3
@23:42:25.78 delay 3 seconds
@23:42:28.85 AS.seek 0.835ml
@23:42:34.12 delay 3 seconds
@23:42:37.15 ATV.dial :bleach
@23:42:37.78 AS.seek 0.82ml
@23:42:41.17 delay 3 seconds
@23:42:44.20 ARV.dial :waste
@23:42:44.70 delay 3 seconds
@23:42:47.73 ATV.dial :ARV
@23:42:48.65 AS.reconfigure ASconfig
@23:42:48.69 AS.seek 0.47ml
@23:43:09.41 AFV.select :carrierSolution
@23:43:09.56 delay 2 seconds
@23:43:11.58 AS.seek 1ml
@23:43:42.34 delay 1 second
@23:43:43.36 AFV.select :holdingCoil
@23:43:43.47 delay 1 second
@23:43:44.49 AS.reconfigure ASfast
@23:43:44.54 AS.seek 0ml
@23:44:11.88 AFV.select :carrierSolution
@23:44:11.99 delay 2 seconds
@23:44:14.01 AS.reconfigure ASconfig
@23:44:14.06 AS.seek 1ml
@23:45:11.83 delay 1 second
@23:45:12.85 AFV.select :holdingCoil
@23:45:12.96 delay 1 second
@23:45:13.98 AS.reconfigure ASfast
@23:45:14.03 AS.seek 0ml
@23:45:41.36 AFV.select :carrierSolution
@23:45:41.47 delay 2 seconds
@23:45:43.49 AS.reconfigure ASconfig
@23:45:43.54 AS.seek 1ml
@23:46:41.27 delay 1 second
@23:46:42.29 AFV.select :holdingCoil
@23:46:42.40 delay 1 second
@23:46:43.42 AS.reconfigure ASfast
@23:46:43.47 AS.seek 0ml
@23:47:10.78 ARV.dial between carrier and air1
@23:47:11.30 ATV.dial :air3
@23:47:12.64 AS.reconfigure ASslow1
@23:47:12.68 AS.seek 0.01ml
@23:47:15.09 delay 2 seconds
@23:47:17.12 ATV.dial :bleach
@23:47:17.75 AS.seek 0.81ml
@23:49:58.86 delay 3 seconds
@23:50:01.89 ATV.dial :air3
@23:50:02.45 delay 3 seconds
@23:50:05.52 AS.seek 0.835ml
@23:50:10.84 delay 3 seconds
@23:50:13.87 ATV.dial :bleach
@23:50:14.50 AS.seek 0.82ml
@23:50:17.80 delay 3 seconds
@23:50:20.83 ATV.dial PCR
@23:50:21.24 AS.reconfigure ASconfig
@23:50:21.28 AS.seek 0ml
@23:51:08.67 PCR.program :bleachProfile
@23:51:08.73 PCR program should complete in < 2:45
@23:51:08.74 PCR.run 10:00
@23:54:07.58 PCR.off
@23:54:07.60 AFV.select :carrierSolution
@23:54:07.71 delay 2 seconds
@23:54:09.73 AS.seek 1ml
@23:55:07.50 delay 1 second
@23:55:08.51 AFV.select :holdingCoil
@23:55:08.62 delay 1 second
@23:55:09.64 AS.reconfigure ASfast
@23:55:09.69 AS.seek 0ml
@23:55:37.03 delay 3 seconds
@23:55:40.05 AFV.select :carrierSolution
@23:55:40.16 delay 2 seconds
@23:55:42.18 AS.reconfigure ASconfig
@23:55:42.23 AS.seek 1ml
@23:56:39.97 delay 1 second
@23:56:40.98 AFV.select :holdingCoil
@23:56:41.09 delay 1 second
@23:56:42.11 AS.reconfigure ASfast
@23:56:42.16 AS.seek 0ml
@23:57:09.50 delay 3 seconds
@23:57:12.53 AFV.select :carrierSolution
@23:57:12.64 delay 2 seconds
@23:57:14.66 AS.reconfigure ASconfig
@23:57:14.72 AS.seek 1ml
@23:58:12.45 delay 1 second
@23:58:13.46 AFV.select :holdingCoil
@23:58:13.57 delay 1 second
@23:58:14.59 AS.reconfigure ASfast
@23:58:14.64 AS.seek 0ml
@23:58:41.95 delay 3 seconds
@23:58:44.97 AFV.select :carrierSolution
@23:58:45.08 delay 2 seconds
@23:58:47.10 AS.reconfigure ASconfig
@23:58:47.14 AS.seek 1ml
@23:59:44.88 delay 1 second
@23:59:45.89 AFV.select :holdingCoil
@23:59:46.00 delay 1 second
@23:59:47.02 AS.reconfigure ASfast
@23:59:47.07 AS.seek 0ml
@00:00:14.38CEST14-Apr-21 delay 3 seconds
@00:00:17.40 AFV.select :carrierSolution
@00:00:17.51 delay 2 seconds
@00:00:19.53 AS.reconfigure ASconfig
@00:00:19.59 AS.seek 1ml
@00:01:17.30 delay 1 second
@00:01:18.31 AFV.select :holdingCoil
@00:01:18.42 delay 1 second
@00:01:19.44 AS.reconfigure ASnorm
@00:01:19.49 AS.seek 0ml
@00:02:10.37 <MFBPCR> Setting up PCR for SYBRIPc -- writing /var/log/esp/SYBRIPc21apr1400h.pcr
@00:02:10.40 Building PCR Slug from elutionWater with MM1 mix, PP6 primer
@00:02:10.41 Priming with carrier
@00:02:10.42 ATV.dial ARV
@00:02:11.14 ARV.dial :carrier
@00:02:11.48 AS.seek 0.5ml
@00:02:36.98 AFV.select :carrierSolution
@00:02:37.09 delay 2 seconds
@00:02:39.11 AS.reconfigure ASconfig
@00:02:39.15 AS.seek 1ml
@00:03:08.16 delay 1 second
@00:03:09.18 AFV.select :holdingCoil
@00:03:09.29 delay 1 second
@00:03:10.68 ATV.dial :waste
@00:03:12.45 AS.reconfigure ASnorm
@00:03:12.49 AS.seek 0ml
@00:04:03.22 AFV.select :carrierSolution
@00:04:03.33 delay 2 seconds
@00:04:05.35 AS.reconfigure ASconfig
@00:04:05.40 AS.seek 0.5ml
@00:04:34.42 delay 1 second
@00:04:35.44 AFV.select :holdingCoil
@00:04:35.55 Building Slug
@00:04:35.56 ATV.dial :ARV
@00:04:37.28 delay 2 seconds
@00:04:39.31 ARV.dial :air1
@00:04:39.71 delay 2 seconds
@00:04:41.78 AS.reconfigure ASslow2
@00:04:41.83 AS.seek 0.506ml
@00:04:48.11 delay 2 seconds
@00:04:50.14 ARV.dial :MM1
@00:04:50.75 delay 2 seconds
@00:04:52.78 Adding MM1
@00:04:52.84 AS.seek 0.524ml
@00:05:11.73 delay 25 seconds
@00:05:36.76 ARV.dial :PP6
@00:05:38.10 Adding PP6
@00:05:38.11 delay 2 seconds
@00:05:40.17 AS.seek 0.53ml
@00:05:46.36 delay 10 seconds
@00:05:56.40 ARV.dial :air1
@00:05:57.26 adding ARV air
@00:05:57.27 delay 2 seconds
@00:05:59.33 AS.seek 0.536ml
@00:06:05.50 delay 5 seconds
@00:06:10.53 ARV.dial :carrier
@00:06:10.92 delay 7 seconds
@00:06:17.99 AS.seek 0.594ml
@00:07:16.50 delay 5 seconds
@00:07:21.53 ATV.dial :elutionWater
@00:07:23.05 delay 2 seconds
@00:07:25.12 AS.seek 0.6ml
@00:07:31.53 delay 4 seconds
@00:07:35.56 ATV.dial ARV
@00:07:37.14 delay 2 seconds
@00:07:39.17 ARV.dial :carrier
@00:07:39.18 delay 2 seconds
@00:07:41.24 AS.seek 0.65ml
@00:08:32.17 delay 4 seconds
@00:08:36.20 ATV.dial PCR
@00:08:36.98 ASV.dial :waste1
@00:08:37.00 ARV.dial :air1
@00:08:37.40 Positioning Slug by 0.324ml
@00:08:37.47 AS.seek 0.326ml
@00:14:03.64 delay 5 seconds
@00:14:08.67 ATV.dial ARV
@00:14:09.47 ASV.dial :PCRlock
@00:14:09.86 delay 5 seconds
@00:14:14.89 Running SYBRIPc Profile60CIQ1 thermocycling
@00:14:14.90 <PCR> PCR.program Profile60CIQ1
@00:14:15.01 Profile60CIQ1 should complete in < 1:56:15
@00:14:15.02 PCR.run 2:30:18.75
@01:53:01.43 PCR.off
@01:53:01.98 <MFBPCR> PCR for SYBRIPc completed -- closed /var/log/esp/SYBRIPc21apr1400h.pcr
@01:53:02.04 Cleaning PCR
@01:53:02.05 AFV.select :carrierSolution
@01:53:02.16 delay 2 seconds
@01:53:04.18 AS.reconfigure ASconfig
@01:53:04.23 AS.seek 0.5ml
@01:53:14.50 delay 1 second
@01:53:15.52 AFV.select :holdingCoil
@01:53:15.63 ASV.dial :waste1
@01:53:16.02 ATV.dial :air3
@01:53:17.36 AS.reconfigure ASslow1
@01:53:17.40 AS.seek 0.51ml
@01:53:19.79 delay 2 seconds
@01:53:21.82 ATV.dial :bleach
@01:53:22.45 AS.seek 0.81ml
@01:54:23.14 delay 3 seconds
@01:54:26.17 ATV.dial :air3
@01:54:26.74 delay 3 seconds
@01:54:29.81 AS.seek 0.835ml
@01:54:35.14 delay 3 seconds
@01:54:38.17 ATV.dial :bleach
@01:54:38.80 AS.seek 0.82ml
@01:54:42.08 delay 3 seconds
@01:54:45.11 ARV.dial :waste
@01:54:45.72 delay 3 seconds
@01:54:48.75 ATV.dial :ARV
@01:54:49.67 AS.reconfigure ASconfig
@01:54:49.71 AS.seek 0.47ml
@01:55:10.10 AFV.select :carrierSolution
@01:55:10.21 delay 2 seconds
@01:55:12.23 AS.seek 1ml
@01:55:43.00 delay 1 second
@01:55:44.02 AFV.select :holdingCoil
@01:55:44.13 delay 1 second
@01:55:45.15 AS.reconfigure ASfast
@01:55:45.20 AS.seek 0ml
@01:56:12.52 AFV.select :carrierSolution
@01:56:12.63 delay 2 seconds
@01:56:14.65 AS.reconfigure ASconfig
@01:56:14.70 AS.seek 1ml
@01:57:12.50 delay 1 second
@01:57:13.52 AFV.select :holdingCoil
@01:57:13.63 delay 1 second
@01:57:14.65 AS.reconfigure ASfast
@01:57:14.70 AS.seek 0ml
@01:57:42.02 AFV.select :carrierSolution
@01:57:42.13 delay 2 seconds
@01:57:44.15 AS.reconfigure ASconfig
@01:57:44.19 AS.seek 1ml
@01:58:41.92 delay 1 second
@01:58:42.94 AFV.select :holdingCoil
@01:58:43.05 delay 1 second
@01:58:44.07 AS.reconfigure ASfast
@01:58:44.12 AS.seek 0ml
@01:59:11.42 ARV.dial between carrier and air1
@01:59:11.94 ATV.dial :air3
@01:59:13.28 AS.reconfigure ASslow1
@01:59:13.32 AS.seek 0.01ml
@01:59:15.61 delay 2 seconds
@01:59:17.64 ATV.dial :bleach
@01:59:18.27 AS.seek 0.81ml
@02:01:59.52 delay 3 seconds
@02:02:02.55 ATV.dial :air3
@02:02:03.11 delay 3 seconds
@02:02:06.18 AS.seek 0.835ml
@02:02:11.48 delay 3 seconds
@02:02:14.51 ATV.dial :bleach
@02:02:15.14 AS.seek 0.82ml
@02:02:18.42 delay 3 seconds
@02:02:21.45 ATV.dial PCR
@02:02:21.87 AS.reconfigure ASconfig
@02:02:21.91 AS.seek 0ml
@02:03:09.28 PCR.program :bleachProfile
@02:03:09.34 PCR program should complete in < 2:45
@02:03:09.35 PCR.run 10:00
@02:06:05.51 PCR.off
@02:06:05.53 AFV.select :carrierSolution
@02:06:05.64 delay 2 seconds
@02:06:07.66 AS.seek 1ml
@02:07:05.41 delay 1 second
@02:07:06.43 AFV.select :holdingCoil
@02:07:06.54 delay 1 second
@02:07:07.56 AS.reconfigure ASfast
@02:07:07.61 AS.seek 0ml
@02:07:34.92 delay 3 seconds
@02:07:37.94 AFV.select :carrierSolution
@02:07:38.05 delay 2 seconds
@02:07:40.07 AS.reconfigure ASconfig
@02:07:40.12 AS.seek 1ml
@02:08:37.86 delay 1 second
@02:08:38.88 AFV.select :holdingCoil
@02:08:38.99 delay 1 second
@02:08:40.01 AS.reconfigure ASfast
@02:08:40.06 AS.seek 0ml
@02:09:07.38 delay 3 seconds
@02:09:10.40 AFV.select :carrierSolution
@02:09:10.51 delay 2 seconds
@02:09:12.53 AS.reconfigure ASconfig
@02:09:12.58 AS.seek 1ml
@02:10:10.31 delay 1 second
@02:10:11.33 AFV.select :holdingCoil
@02:10:11.44 delay 1 second
@02:10:12.46 AS.reconfigure ASfast
@02:10:12.51 AS.seek 0ml
@02:10:39.82 delay 3 seconds
@02:10:42.84 AFV.select :carrierSolution
@02:10:42.95 delay 2 seconds
@02:10:44.97 AS.reconfigure ASconfig
@02:10:45.02 AS.seek 1ml
@02:11:42.80 delay 1 second
@02:11:43.82 AFV.select :holdingCoil
@02:11:43.93 delay 1 second
@02:11:44.95 AS.reconfigure ASfast
@02:11:45.00 AS.seek 0ml
@02:12:12.32 delay 3 seconds
@02:12:15.34 AFV.select :carrierSolution
@02:12:15.45 delay 2 seconds
@02:12:17.47 AS.reconfigure ASconfig
@02:12:17.53 AS.seek 1ml
@02:13:15.30 delay 1 second
@02:13:16.32 AFV.select :holdingCoil
@02:13:16.43 delay 1 second
@02:13:17.45 AS.reconfigure ASnorm
@02:13:17.50 AS.seek 0ml
@15:18:23.09CEST15-Apr-21 <MAIN> ESP2 v3.30 I2C Gateway v3.06 for core bus
@15:18:25.18 
Configuration for mer 7/20/20 brent@mbari.org
/home/esp/esp2/type/shallow/mer/configure.rb
@15:18:25.27 
Configuration for MFB3 by preston@mbari.org
/home/esp/esp2/type/mfb/MFB3/configure.rb
@15:18:29.61 Polling Can every 10:00
@15:18:31.33 CS.configure :default
@15:18:31.37 CS.hold
@15:18:31.44 CS.configure :default
@15:18:31.52 PS.configure :default
@15:18:31.60 SS.configure :default
@15:18:31.64 SS.hold
@15:18:31.69 SS.configure :default
@15:18:31.77 CC.configure :default
@15:18:31.80 CC.hold
@15:18:31.88 CC.configure :default
@15:18:31.96 PC.configure :default
@15:18:32.00 PC.hold
@15:18:32.05 PC.configure :default
@15:18:32.15 Forearm.configure :loose
@15:18:32.19 Forearm.hold
@15:18:32.25 Forearm.configure :loose
@15:18:32.33 Elbow.configure :loose
@15:18:32.37 Elbow.hold
@15:18:32.42 Elbow.configure :loose
@15:18:32.52 SC.configure :loose
@15:18:32.56 SC.hold
@15:18:32.61 SC.configure :loose
@15:18:32.69 SE.configure :default
@15:18:32.73 SE.hold
@15:18:32.78 SE.configure :default
@15:18:32.82 CH.coast
@15:18:32.86 CH.configure :default
@15:18:32.89 PH.coast
@15:18:32.94 PH.configure :default
@15:18:33.04 SPE.coast
@15:18:33.08 SPE.configure SPEconfig
@15:18:33.15 AS.configure ASconfig
@15:18:33.21 AS.hold
@15:18:33.31 AS.configure ASconfig
@15:18:33.40 PCR.off
@15:18:33.45 PCR.pidtemp= 50,0.1,0,300,25,25
@15:18:33.50 PCR.caltemp= 2.5051,0.0372,0
@15:18:33.55 All dwarf controllers running firmware version 3.75
@15:18:33.89 Cleanly exited at 15:15:12CEST15-Apr-21
@15:18:33.95 Loading /home/esp/esp2/mission/GENOMAP_v3.rb mission
@15:18:34.21 <MFBPCR> Setting up PCR for ACYPP2 -- writing /var/log/esp/ACYPP221apr1515h.pcr
@15:18:34.24 Building PCR Slug from ASV with MM1 mix, PP2 primer
@15:18:34.27 <mwj> Session Begun
@15:18:34.31 <MFBPCR> Priming with carrier
@15:18:34.35 ATV.dial ARV
@15:18:36.12 ARV.dial :carrier
@15:18:36.47 AS.reconfigure ASnorm
@15:18:36.53 AS.seek 0.5ml
@15:19:02.05 AFV.select :carrierSolution
@15:19:02.18 delay 2 seconds
@15:19:04.20 AS.reconfigure ASconfig
@15:19:04.27 AS.seek 1ml
@15:19:33.29 delay 1 second
@15:19:34.31 AFV.select :holdingCoil
@15:19:34.42 delay 1 second
@15:19:35.44 ATV.dial :waste
@15:19:37.22 AS.reconfigure ASnorm
@15:19:37.28 AS.seek 0ml
@15:20:27.99 AFV.select :carrierSolution
@15:20:28.11 delay 2 seconds
@15:20:30.13 AS.reconfigure ASconfig
@15:20:30.19 AS.seek 0.5ml
@15:20:59.23 delay 1 second
@15:21:00.25 AFV.select :holdingCoil
@15:21:00.36 Building Slug
@15:21:00.37 ATV.dial :ARV
@15:21:02.12 delay 2 seconds
@15:21:04.15 ARV.dial :air1
@15:21:04.55 delay 2 seconds
@15:21:06.62 AS.reconfigure ASslow2
@15:21:06.68 AS.seek 0.506ml
@15:21:12.90 delay 2 seconds
@15:21:14.93 ARV.dial :MM1
@15:21:15.57 delay 2 seconds
@15:21:17.60 Adding MM1
@15:21:17.67 AS.seek 0.524ml
@15:21:35.87 delay 25 seconds
@15:21:43.28 <mwj> -> abort! "error in scrip"
@15:21:43.30 CmdServer::Client::Error in mwj -- Aborting Mission: error in scrip
@15:21:43.74 <Qmail> BadNews.email "error in scrip",Subject: "Mission ABORTED!"
@15:21:51.84 <mwj> Session Ended
@15:49:37.18CEST15-Apr-21 <MAIN> ESP2 v3.30 I2C Gateway v3.06 for core bus
@15:49:39.69 
Configuration for mer 7/20/20 brent@mbari.org
/home/esp/esp2/type/shallow/mer/configure.rb
@15:49:39.78 
Configuration for MFB3 by preston@mbari.org
/home/esp/esp2/type/mfb/MFB3/configure.rb
@15:49:44.94 Polling Can every 10:00
@15:49:46.81 CS.configure :default
@15:49:46.85 CS.hold
@15:49:46.93 CS.configure :default
@15:49:47.02 PS.configure :default
@15:49:47.10 SS.configure :default
@15:49:47.14 SS.hold
@15:49:47.20 SS.configure :default
@15:49:47.29 CC.configure :default
@15:49:47.32 CC.hold
@15:49:47.39 CC.configure :default
@15:49:47.47 PC.configure :default
@15:49:47.51 PC.hold
@15:49:47.56 PC.configure :default
@15:49:47.66 Forearm.configure :loose
@15:49:47.70 Forearm.hold
@15:49:47.75 Forearm.configure :loose
@15:49:47.83 Elbow.configure :loose
@15:49:47.87 Elbow.hold
@15:49:47.92 Elbow.configure :loose
@15:49:48.02 SC.configure :loose
@15:49:48.06 SC.hold
@15:49:48.11 SC.configure :loose
@15:49:48.18 SE.configure :default
@15:49:48.22 SE.hold
@15:49:48.26 SE.configure :default
@15:49:48.30 CH.coast
@15:49:48.35 CH.configure :default
@15:49:48.37 PH.coast
@15:49:48.42 PH.configure :default
@15:49:48.52 SPE.coast
@15:49:48.57 SPE.configure SPEconfig
@15:49:48.64 AS.configure ASconfig
@15:49:48.68 AS.hold
@15:49:48.72 AS.configure ASconfig
@15:49:48.77 PCR.off
@15:49:48.81 PCR.pidtemp= 50,0.1,0,300,25,25
@15:49:48.84 PCR.caltemp= 2.5051,0.0372,0
@15:49:48.87 All dwarf controllers running firmware version 3.75
@15:49:49.08 Cleanly exited at 15:49:16CEST15-Apr-21
@15:49:49.11 Loading /home/esp/esp2/mission/GENOMAP_v3.rb mission
@15:49:49.26 <MFBPCR> Extracting Nucleic Acid from extSrc
@15:49:49.28 SPE.hold 2:46:40,55C
@15:49:49.32 ASV.dial :SPEdelivery
@15:49:49.75 delay 20 seconds
@15:49:50.05 <mwj> Session Begun
@15:50:09.79 <MFBPCR> AFV.select :holdingCoil
@15:50:09.91 ATV.dial :air2
@15:50:11.22 AS.reconfigure ASslow1
@15:50:11.28 AS.seek 0.07ml
@15:50:25.76 delay 3 seconds
@15:50:28.79 ATV.dial :extSrc
@15:50:30.32 AS.seek 0.47ml
@15:51:51.13 delay 5 seconds
@15:51:56.21 Solid Phase Extraction Heater at 39.5C
@15:51:56.22 ATV.dial ASV
@15:51:57.09 ASV.dial :SPEdelivery
@15:51:57.18 AS.seek 0.042ml
@15:53:23.54 delay 10 seconds
@15:53:33.58 Rinsing external lysate line extSrc
@15:53:33.59 AFV.select :carrierSolution
@15:53:33.71 delay 2 seconds
@15:53:35.73 AS.reconfigure ASconfig
@15:53:35.79 AS.seek 1ml
@15:54:31.18 delay 1 second
@15:54:32.20 AFV.select :holdingCoil
@15:54:32.31 delay 1 second
@15:54:33.33 ATV.dial :extSrc
@15:54:34.16 AS.reconfigure ASnorm
@15:54:34.21 AS.seek 0ml
@15:55:24.94 ATV.dial :air2
@15:55:26.54 AS.reconfigure ASslow0
@15:55:26.60 AS.seek 0.05ml
@15:55:32.01 ATV.dial :extSrc
@15:55:33.61 AS.seek 0.01ml
@15:55:38.01 ATV.dial :waste
@15:55:39.74 AS.reconfigure ASconfig
@15:55:39.84 AS.seek 0ml
@15:55:40.71 ethanol wash
@15:55:40.72 ATV.dial :air2
@15:55:41.91 AS.reconfigure ASslow2
@15:55:41.97 AS.seek 0.01ml
@15:55:52.77 delay 3 seconds
@15:55:55.80 Pulling 0.4ml of Ethanol Wash
@15:55:55.81 ATV.dial :ethanol
@15:55:56.71 AS.reconfigure ASslow1
@15:55:56.77 AS.seek 0.41ml
@15:57:17.46 delay 3 seconds
@15:57:20.49 ATV.dial :air1
@15:57:20.91 delay 3 seconds
@15:57:23.98 AS.seek 0.435ml
@15:57:29.37 delay 3 seconds
@15:57:32.40 ATV.dial :ethanol
@15:57:33.02 AS.seek 0.42ml
@15:57:36.30 delay 3 seconds
@15:57:39.33 ATV.dial ASV
@15:57:40.74 AS.seek 0.093ml
@15:58:46.85 delay 3 seconds
@15:58:49.88 ATV.dial :waste2
@15:58:50.69 AS.reconfigure ASconfig
@15:58:50.74 AS.seek 0ml
@15:58:56.38 ATV.dial :air2
@15:58:56.81 AS.reconfigure ASslow1
@15:58:56.86 AS.seek 1ml
@16:02:18.24 delay 5 seconds
@16:02:23.27 ATV.dial ASV
@16:02:24.30 ASV.dial :SPEdelivery
@16:02:24.32 AS.seek 0.02ml
@16:05:41.71 delay 5 seconds
@16:05:46.74 ATV.dial :air2
@16:05:47.83 AS.seek 0.52ml
@16:07:28.66 delay 5 seconds
@16:07:33.69 ATV.dial ASV
@16:07:34.73 ASV.dial :wasteR
@16:07:35.13 AS.seek 0.3ml
@16:08:19.66 delay 20 seconds
@16:08:39.70 AS.seek 0.07ml
@16:09:26.23 delay 20 seconds
@16:09:46.26 ATV.dial :waste2
@16:09:47.06 AS.reconfigure ASconfig
@16:09:47.11 AS.seek 0ml
@16:09:51.43 Elution
@16:09:51.44 ATV.dial :air1
@16:09:52.65 AS.reconfigure ASslow1
@16:09:52.70 AS.seek 0.05ml
@16:10:03.09 delay 3 seconds
@16:10:06.12 ATV.dial :ElutionWater
@16:10:06.85 AS.seek 0.11ml
@16:10:19.18 delay 3 seconds
@16:10:22.21 ATV.dial ASV,via: :air2
@16:10:24.47 ASV.dial :SPEelution
@16:10:25.09 AFV.select :carrierSolution
@16:10:25.25 AS.reconfigure ASnorm
@16:10:25.31 AS.seek 0.76ml
@16:10:58.34 delay 3 seconds
@16:11:01.36 AFV.select :holdingCoil
@16:11:01.51 AS.reconfigure ASslow1
@16:11:01.57 AS.seek 0.475ml
@16:11:59.16 delay 5 seconds
@16:12:04.24 AS.reconfigure ASslow2
@16:12:04.30 AS.seek 0.44ml
@16:12:39.76 delay 1:00
@16:13:39.80 pushing eluate to holding coil
@16:13:39.87 AS.seek 0.28ml
@16:16:21.15 delay 30 seconds
@16:16:51.19 ATV.dial :waste,via: :air3
@16:16:53.05 AS.reconfigure ASconfig
@16:16:53.10 AS.seek 0ml
@16:17:09.51 Parking Bleach in LysateSource line and SPEcolumn
@16:17:09.52 ATV.dial :air3
@16:17:10.43 AS.reconfigure ASslow1
@16:17:10.48 AS.seek 0.03ml
@16:17:16.91 ATV.dial :ElutionWater
@16:17:17.55 AS.seek 0.06ml
@16:17:23.87 ATV.dial :air3
@16:17:24.52 AS.seek 0.07ml
@16:17:26.77 delay 2 seconds
@16:17:28.80 ATV.dial :bleach
@16:17:29.27 AS.seek 0.15ml
@16:17:45.62 delay 3 seconds
@16:17:48.65 ATV.dial :air3
@16:17:49.24 delay 3 seconds
@16:17:52.31 AS.seek 0.175ml
@16:17:57.69 delay 3 seconds
@16:18:00.72 ATV.dial :bleach
@16:18:01.36 AS.seek 0.16ml
@16:18:04.77 delay 3 seconds
@16:18:07.80 ATV.dial :extSrc
@16:18:08.45 AS.reconfigure ASconfig
@16:18:08.50 AS.seek 0.015ml
@16:18:17.15 ATV.dial :waste
@16:18:18.73 AS.seek 0ml
@16:18:19.88 ATV.dial :air3
@16:18:20.75 AS.reconfigure ASslow1
@16:18:20.80 AS.seek 0.01ml
@16:18:23.10 delay 2 seconds
@16:18:25.13 ATV.dial :bleach
@16:18:25.61 AS.seek 0.58ml
@16:20:20.60 delay 3 seconds
@16:20:23.63 ATV.dial :air3
@16:20:24.23 delay 3 seconds
@16:20:27.30 AS.seek 0.605ml
@16:20:32.70 delay 3 seconds
@16:20:35.73 ATV.dial :bleach
@16:20:36.38 AS.seek 0.59ml
@16:20:39.66 delay 3 seconds
@16:20:42.69 ATV.dial :ASV
@16:20:43.79 ASV.dial :wasteR
@16:20:44.42 AS.seek 0ml
@16:22:43.44 delay 1:00
@16:23:43.47 AFV.select :carrierSolution
@16:23:43.58 delay 2 seconds
@16:23:45.60 AS.reconfigure ASconfig
@16:23:45.65 AS.seek 1ml
@16:24:43.40 delay 1 second
@16:24:44.42 AFV.select :holdingCoil
@16:24:44.53 delay 1 second
@16:24:45.55 ATV.dial ASV
@16:24:45.56 ASV.dial :wasteR
@16:24:45.59 AS.reconfigure ASslow1
@16:24:45.64 AS.seek 0ml
@16:28:07.04 delay 20 seconds
@16:28:27.08 ATV.dial :air3
@16:28:28.71 AS.seek 0.59ml
@16:30:27.57 delay 3 seconds
@16:30:30.60 ATV.dial :ASV
@16:30:32.13 ASV.dial :wasteR
@16:30:32.22 AS.seek 0.09ml
@16:32:13.02 delay 30 seconds
@16:32:43.06 ATV.dial :extSrc
@16:32:43.88 delay 3 seconds
@16:32:46.91 ATV.dial :ElutionWater
@16:32:48.11 AS.seek 0.12ml
@16:32:54.54 ATV.dial :air3
@16:32:55.19 AS.seek 0.13ml
@16:32:57.48 delay 2 seconds
@16:32:59.51 ATV.dial :bleach
@16:33:00.00 AS.seek 0.23ml
@16:33:20.38 delay 3 seconds
@16:33:23.41 ATV.dial :air3
@16:33:23.98 delay 3 seconds
@16:33:27.05 AS.seek 0.255ml
@16:33:32.45 delay 3 seconds
@16:33:35.48 ATV.dial :bleach
@16:33:36.54 AS.seek 0.24ml
@16:33:39.87 delay 3 seconds
@16:33:42.90 ATV.dial :ASV
@16:33:44.01 ASV.dial :SPEdelivery
@16:33:44.41 AFV.select :carrierSolution
@16:33:44.53 delay 2 seconds
@16:33:46.55 AS.reconfigure ASconfig
@16:33:46.60 AS.seek 0.5ml
@16:34:01.82 delay 1 second
@16:34:02.84 AFV.select :holdingCoil
@16:34:02.99 AS.reconfigure ASslow1
@16:34:03.05 AS.seek 0.17ml
@16:35:09.72 SPE.hold 2:46:40,95C
@16:35:09.78 delay 3:00
@16:38:09.82 AFV.select :carrierSolution
@16:38:09.94 delay 2 seconds
@16:38:11.96 AS.reconfigure ASconfig
@16:38:12.01 AS.seek 1ml
@16:39:00.01 delay 1 second
@16:39:01.03 AFV.select :holdingCoil
@16:39:01.14 delay 1 second
@16:39:02.16 ASV.dial :wasteD
@16:39:03.28 AS.seek 0ml
@16:40:01.05 Priming Eluate
@16:40:01.06 AFV.select :carrierSolution
@16:40:01.19 delay 2 seconds
@16:40:03.21 AS.seek 0.5ml
@16:40:32.24 delay 1 second
@16:40:33.26 AFV.select :holdingCoil
@16:40:33.37 ATV.dial ASV
@16:40:33.38 ASV.dial :SPErecovery
@16:40:34.04 Swish #1 of 2
@16:40:34.12 AS.reconfigure ASslow0
@16:40:34.18 AS.seek 0.6ml
@16:40:44.63 AS.seek 0.5ml
@16:40:55.02 Swish #2 of 2
@16:40:55.11 AS.seek 0.6ml
@16:41:05.60 AS.seek 0.5ml
@16:41:15.93 delay 3 seconds
@16:41:19.00 AS.reconfigure ASslow1
@16:41:19.06 AS.seek 0.684ml
@16:41:56.34 delay 3 seconds
@16:41:59.37 Eluate primed
@16:41:59.38 ATV.dial :waste2
@16:42:00.06 AS.reconfigure ASnorm
@16:42:00.11 AS.seek 0ml
@16:42:34.90 SPE.coast
@16:42:34.98 ASV.dial :SPErecovery
@16:42:35.00 Setting up PCR for SYBRIPc -- writing /var/log/esp/SYBRIPc21apr1516h.pcr
@16:42:35.03 Building PCR Slug from ASV with MM1 mix, PP6 primer
@16:42:35.05 Priming with carrier
@16:42:35.07 ATV.dial ARV
@16:42:36.12 ARV.dial :carrier
@16:42:36.47 AS.seek 0.5ml
@16:43:02.05 AFV.select :carrierSolution
@16:43:02.18 delay 2 seconds
@16:43:04.20 AS.reconfigure ASconfig
@16:43:04.26 AS.seek 1ml
@16:43:33.26 delay 1 second
@16:43:34.29 AFV.select :holdingCoil
@16:43:34.39 delay 1 second
@16:43:35.41 ATV.dial :waste
@16:43:37.05 AS.reconfigure ASnorm
@16:43:37.11 AS.seek 0ml
@16:44:27.79 AFV.select :carrierSolution
@16:44:27.91 delay 2 seconds
@16:44:29.93 AS.reconfigure ASconfig
@16:44:29.98 AS.seek 0.5ml
@16:44:59.02 delay 1 second
@16:45:00.04 AFV.select :holdingCoil
@16:45:00.15 Building Slug
@16:45:00.16 ATV.dial :ARV
@16:45:01.91 delay 2 seconds
@16:45:03.94 ARV.dial :air1
@16:45:04.35 delay 2 seconds
@16:45:06.42 AS.reconfigure ASslow2
@16:45:06.48 AS.seek 0.506ml
@16:45:12.93 delay 2 seconds
@16:45:14.96 ARV.dial :MM1
@16:45:15.60 delay 2 seconds
@16:45:17.63 Adding MM1
@16:45:17.70 AS.seek 0.524ml
@16:45:36.07 delay 25 seconds
@16:46:01.10 ARV.dial :PP6
@16:46:02.45 Adding PP6
@16:46:02.46 delay 2 seconds
@16:46:04.54 AS.seek 0.53ml
@16:46:10.77 delay 10 seconds
@16:46:20.81 ARV.dial :air1
@16:46:21.69 adding ARV air
@16:46:21.70 delay 2 seconds
@16:46:23.77 AS.seek 0.536ml
@16:46:30.26 delay 5 seconds
@16:46:35.29 ARV.dial :carrier
@16:46:35.69 delay 7 seconds
@16:46:42.77 AS.seek 0.594ml
@16:47:41.68 delay 5 seconds
@16:47:46.71 ATV.dial :ASV
@16:47:47.13 delay 2 seconds
@16:47:49.20 AS.seek 0.6ml
@16:47:55.99 delay 4 seconds
@16:48:00.02 ATV.dial ARV
@16:48:00.59 delay 2 seconds
@16:48:02.62 ARV.dial :carrier
@16:48:02.63 delay 2 seconds
@16:48:04.71 AS.seek 0.65ml
@16:48:55.98 delay 4 seconds
@16:49:00.01 ATV.dial PCR
@16:49:00.68 ASV.dial :waste1
@16:49:01.49 ARV.dial :air1
@16:49:01.91 Positioning Slug by 0.324ml
@16:49:01.99 AS.seek 0.326ml
@16:54:27.91 delay 5 seconds
@16:54:32.94 ATV.dial ARV
@16:54:33.76 ASV.dial :PCRlock
@16:54:34.15 delay 5 seconds
@16:54:39.18 Running SYBRIPc Profile60CIQ1 thermocycling
@16:54:39.19 <PCR> PCR.program Profile60CIQ1
@16:54:39.31 Profile60CIQ1 should complete in < 1:56:15
@16:54:39.32 PCR.run 2:30:18.75
@18:33:51.21 PCR.off
@18:33:51.78 <MFBPCR> PCR for SYBRIPc completed -- closed /var/log/esp/SYBRIPc21apr1516h.pcr
@18:33:51.85 Cleaning PCR
@18:33:51.86 AFV.select :carrierSolution
@18:33:51.98 delay 2 seconds
@18:33:54.00 AS.reconfigure ASconfig
@18:33:54.05 AS.seek 0.5ml
@18:34:04.35 delay 1 second
@18:34:05.37 AFV.select :holdingCoil
@18:34:05.48 ASV.dial :waste1
@18:34:05.87 ATV.dial :air3
@18:34:07.24 AS.reconfigure ASslow1
@18:34:07.30 AS.seek 0.51ml
@18:34:09.56 delay 2 seconds
@18:34:11.59 ATV.dial :bleach
@18:34:12.24 AS.seek 0.81ml
@18:35:12.91 delay 3 seconds
@18:35:15.94 ATV.dial :air3
@18:35:16.52 delay 3 seconds
@18:35:19.59 AS.seek 0.835ml
@18:35:24.91 delay 3 seconds
@18:35:27.94 ATV.dial :bleach
@18:35:28.58 AS.seek 0.82ml
@18:35:31.91 delay 3 seconds
@18:35:34.94 ARV.dial :waste
@18:35:35.57 delay 3 seconds
@18:35:38.60 ATV.dial :ARV
@18:35:39.54 AS.reconfigure ASconfig
@18:35:39.60 AS.seek 0.47ml
@18:35:59.98 AFV.select :carrierSolution
@18:36:00.10 delay 2 seconds
@18:36:02.12 AS.seek 1ml
@18:36:32.87 delay 1 second
@18:36:33.89 AFV.select :holdingCoil
@18:36:34.00 delay 1 second
@18:36:35.02 AS.reconfigure ASfast
@18:36:35.07 AS.seek 0ml
@18:37:02.38 AFV.select :carrierSolution
@18:37:02.50 delay 2 seconds
@18:37:04.52 AS.reconfigure ASconfig
@18:37:04.57 AS.seek 1ml
@18:38:02.30 delay 1 second
@18:38:03.32 AFV.select :holdingCoil
@18:38:03.43 delay 1 second
@18:38:04.45 AS.reconfigure ASfast
@18:38:04.50 AS.seek 0ml
@18:38:31.85 AFV.select :carrierSolution
@18:38:31.97 delay 2 seconds
@18:38:33.99 AS.reconfigure ASconfig
@18:38:34.04 AS.seek 1ml
@18:39:32.19 delay 1 second
@18:39:33.21 AFV.select :holdingCoil
@18:39:33.32 delay 1 second
@18:39:34.34 AS.reconfigure ASfast
@18:39:34.40 AS.seek 0ml
@18:40:01.76 ARV.dial between carrier and air1
@18:40:02.30 ATV.dial :air3
@18:40:03.66 AS.reconfigure ASslow1
@18:40:03.72 AS.seek 0.01ml
@18:40:06.02 delay 2 seconds
@18:40:08.05 ATV.dial :bleach
@18:40:08.71 AS.seek 0.81ml
@18:42:49.85 delay 3 seconds
@18:42:52.88 ATV.dial :air3
@18:42:53.46 delay 3 seconds
@18:42:56.53 AS.seek 0.835ml
@18:43:01.86 delay 3 seconds
@18:43:04.89 ATV.dial :bleach
@18:43:05.54 AS.seek 0.82ml
@18:43:08.85 delay 3 seconds
@18:43:11.88 ATV.dial PCR
@18:43:12.31 AS.reconfigure ASconfig
@18:43:12.37 AS.seek 0ml
@18:43:59.80 PCR.program :bleachProfile
@18:43:59.88 PCR program should complete in < 2:45
@18:43:59.89 PCR.run 10:00
@18:46:55.68 PCR.off
@18:46:55.72 AFV.select :carrierSolution
@18:46:55.83 delay 2 seconds
@18:46:57.85 AS.seek 1ml
@18:47:55.65 delay 1 second
@18:47:56.67 AFV.select :holdingCoil
@18:47:56.78 delay 1 second
@18:47:57.80 AS.reconfigure ASfast
@18:47:57.85 AS.seek 0ml
@18:48:25.18 delay 3 seconds
@18:48:28.20 AFV.select :carrierSolution
@18:48:28.31 delay 2 seconds
@18:48:30.33 AS.reconfigure ASconfig
@18:48:30.39 AS.seek 1ml
@18:49:28.13 delay 1 second
@18:49:29.15 AFV.select :holdingCoil
@18:49:29.26 delay 1 second
@18:49:30.28 AS.reconfigure ASfast
@18:49:30.33 AS.seek 0ml
@18:49:57.66 delay 3 seconds
@18:50:00.68 AFV.select :carrierSolution
@18:50:00.79 delay 2 seconds
@18:50:02.81 AS.reconfigure ASconfig
@18:50:02.88 AS.seek 1ml
@18:51:00.62 delay 1 second
@18:51:01.64 AFV.select :holdingCoil
@18:51:01.75 delay 1 second
@18:51:02.77 AS.reconfigure ASfast
@18:51:02.82 AS.seek 0ml
@18:51:30.16 delay 3 seconds
@18:51:33.18 AFV.select :carrierSolution
@18:51:33.29 delay 2 seconds
@18:51:35.31 AS.reconfigure ASconfig
@18:51:35.37 AS.seek 1ml
@18:52:33.16 delay 1 second
@18:52:34.18 AFV.select :holdingCoil
@18:52:34.29 delay 1 second
@18:52:35.31 AS.reconfigure ASfast
@18:52:35.36 AS.seek 0ml
@18:53:02.69 delay 3 seconds
@18:53:05.71 AFV.select :carrierSolution
@18:53:05.82 delay 2 seconds
@18:53:07.84 AS.reconfigure ASconfig
@18:53:07.91 AS.seek 1ml
@18:54:05.66 delay 1 second
@18:54:06.68 AFV.select :holdingCoil
@18:54:06.79 delay 1 second
@18:54:07.81 AS.reconfigure ASnorm
@18:54:07.86 AS.seek 0ml
@18:54:58.58 Setting up PCR for ACYPP2 -- writing /var/log/esp/ACYPP221apr1518h.pcr
@18:54:58.60 Building PCR Slug from ASV with MM1 mix, PP2 primer
@18:54:58.62 Priming with carrier
@18:54:58.64 ATV.dial ARV
@18:54:59.29 ARV.dial :carrier
@18:54:59.64 AS.seek 0.5ml
@18:55:25.21 AFV.select :carrierSolution
@18:55:25.33 delay 2 seconds
@18:55:27.35 AS.reconfigure ASconfig
@18:55:27.41 AS.seek 1ml
@18:55:56.43 delay 1 second
@18:55:57.45 AFV.select :holdingCoil
@18:55:57.56 delay 1 second
@18:55:58.58 ATV.dial :waste
@18:56:00.38 AS.reconfigure ASnorm
@18:56:00.44 AS.seek 0ml
@18:56:51.18 AFV.select :carrierSolution
@18:56:51.66 delay 2 seconds
@18:56:53.75 AS.reconfigure ASconfig
@18:56:53.81 AS.seek 0.5ml
@18:57:22.83 delay 1 second
@18:57:23.85 AFV.select :holdingCoil
@18:57:23.96 Building Slug
@18:57:23.97 ATV.dial :ARV
@18:57:25.74 delay 2 seconds
@18:57:27.76 ARV.dial :air1
@18:57:28.15 delay 2 seconds
@18:57:30.22 AS.reconfigure ASslow2
@18:57:30.28 AS.seek 0.506ml
@18:57:36.51 delay 2 seconds
@18:57:38.54 ARV.dial :MM1
@18:57:39.18 delay 2 seconds
@18:57:41.21 Adding MM1
@18:57:41.28 AS.seek 0.524ml
@18:57:59.74 delay 25 seconds
@18:58:24.77 ARV.dial :PP2
@18:58:25.19 Adding PP2
@18:58:25.20 delay 2 seconds
@18:58:27.28 AS.seek 0.53ml
@18:58:33.62 delay 10 seconds
@18:58:43.66 ARV.dial :air2
@18:58:44.08 adding ARV air
@18:58:44.09 delay 2 seconds
@18:58:46.17 AS.seek 0.536ml
@18:58:52.54 delay 5 seconds
@18:58:57.57 ARV.dial :carrier
@18:58:58.91 delay 7 seconds
@18:59:06.00 AS.seek 0.594ml
@19:00:04.87 delay 5 seconds
@19:00:09.90 ATV.dial :ASV
@19:00:10.32 delay 2 seconds
@19:00:12.40 AS.seek 0.6ml
@19:00:18.69 delay 4 seconds
@19:00:22.72 ATV.dial ARV
@19:00:23.30 delay 2 seconds
@19:00:25.33 ARV.dial :carrier
@19:00:25.34 delay 2 seconds
@19:00:27.42 AS.seek 0.65ml
@19:01:18.21 delay 4 seconds
@19:01:22.24 ATV.dial PCR
@19:01:22.88 ASV.dial :waste1
@19:01:22.90 ARV.dial :air1
@19:01:23.32 Positioning Slug by 0.324ml
@19:01:23.40 AS.seek 0.326ml
@19:06:49.60 delay 5 seconds
@19:06:54.63 ATV.dial ARV
@19:06:55.44 ASV.dial :PCRlock
@19:06:55.85 delay 5 seconds
@19:07:00.88 Running ACYPP2 Profile60CIQ2 thermocycling
@19:07:00.89 <PCR> PCR.program Profile60CIQ2
@19:07:01.02 Profile60CIQ2 should complete in < 1:47:50
@19:07:01.03 PCR.run 2:19:47.5
@20:35:39.46 PCR.off
@20:35:40.09 <MFBPCR> Running ACYPP2 MeltProfile60 thermocycling
@20:35:40.11 <PCR> PCR.program MeltProfile60
@20:35:40.21 MeltProfile60 should complete in < 11:55
@20:35:40.22 PCR.run 19:53.75
@20:43:14.67 PCR.off
@20:43:15.62 <MFBPCR> PCR for ACYPP2 completed -- closed /var/log/esp/ACYPP221apr1518h.pcr
@20:43:15.70 Cleaning PCR
@20:43:15.71 AFV.select :carrierSolution
@20:43:15.83 delay 2 seconds
@20:43:17.85 AS.reconfigure ASconfig
@20:43:17.90 AS.seek 0.5ml
@20:43:28.19 delay 1 second
@20:43:29.21 AFV.select :holdingCoil
@20:43:29.32 ASV.dial :waste1
@20:43:29.71 ATV.dial :air3
@20:43:31.46 AS.reconfigure ASslow1
@20:43:31.52 AS.seek 0.51ml
@20:43:34.02 delay 2 seconds
@20:43:36.05 ATV.dial :bleach
@20:43:36.69 AS.seek 0.81ml
@20:44:37.27 delay 3 seconds
@20:44:40.30 ATV.dial :air3
@20:44:40.88 delay 3 seconds
@20:44:43.95 AS.seek 0.835ml
@20:44:49.24 delay 3 seconds
@20:44:52.27 ATV.dial :bleach
@20:44:52.93 AS.seek 0.82ml
@20:44:56.26 delay 3 seconds
@20:44:59.29 ARV.dial :waste
@20:44:59.91 delay 3 seconds
@20:45:02.94 ATV.dial :ARV
@20:45:03.86 AS.reconfigure ASconfig
@20:45:03.92 AS.seek 0.47ml
@20:45:24.33 AFV.select :carrierSolution
@20:45:24.45 delay 2 seconds
@20:45:26.47 AS.seek 1ml
@20:45:57.19 delay 1 second
@20:45:58.21 AFV.select :holdingCoil
@20:45:58.32 delay 1 second
@20:45:59.34 AS.reconfigure ASfast
@20:45:59.40 AS.seek 0ml
@20:46:26.74 AFV.select :carrierSolution
@20:46:26.86 delay 2 seconds
@20:46:28.88 AS.reconfigure ASconfig
@20:46:28.94 AS.seek 1ml
@20:47:26.69 delay 1 second
@20:47:27.71 AFV.select :holdingCoil
@20:47:27.82 delay 1 second
@20:47:28.84 AS.reconfigure ASfast
@20:47:28.89 AS.seek 0ml
@20:47:56.21 AFV.select :carrierSolution
@20:47:56.33 delay 2 seconds
@20:47:58.35 AS.reconfigure ASconfig
@20:47:58.40 AS.seek 1ml
@20:48:56.19 delay 1 second
@20:48:57.21 AFV.select :holdingCoil
@20:48:57.32 delay 1 second
@20:48:58.34 AS.reconfigure ASfast
@20:48:58.39 AS.seek 0ml
@20:49:25.73 ARV.dial between carrier and air1
@20:49:26.26 ATV.dial :air3
@20:49:27.61 AS.reconfigure ASslow1
@20:49:27.67 AS.seek 0.01ml
@20:49:29.94 delay 2 seconds
@20:49:31.97 ATV.dial :bleach
@20:49:32.61 AS.seek 0.81ml
@20:52:13.76 delay 3 seconds
@20:52:16.79 ATV.dial :air3
@20:52:17.37 delay 3 seconds
@20:52:20.44 AS.seek 0.835ml
@20:52:25.75 delay 3 seconds
@20:52:28.78 ATV.dial :bleach
@20:52:29.43 AS.seek 0.82ml
@20:52:32.76 delay 3 seconds
@20:52:35.79 ATV.dial PCR
@20:52:36.22 AS.reconfigure ASconfig
@20:52:36.27 AS.seek 0ml
@20:53:23.68 PCR.program :bleachProfile
@20:53:23.75 PCR program should complete in < 2:45
@20:53:23.76 PCR.run 10:00
@20:56:19.97 PCR.off
@20:56:20.02 AFV.select :carrierSolution
@20:56:20.13 delay 2 seconds
@20:56:22.15 AS.seek 1ml
@20:57:19.90 delay 1 second
@20:57:20.92 AFV.select :holdingCoil
@20:57:21.03 delay 1 second
@20:57:22.05 AS.reconfigure ASfast
@20:57:22.11 AS.seek 0ml
@20:57:49.44 delay 3 seconds
@20:57:52.46 AFV.select :carrierSolution
@20:57:52.57 delay 2 seconds
@20:57:54.59 AS.reconfigure ASconfig
@20:57:54.66 AS.seek 1ml
@20:58:52.43 delay 1 second
@20:58:53.45 AFV.select :holdingCoil
@20:58:53.56 delay 1 second
@20:58:54.58 AS.reconfigure ASfast
@20:58:54.64 AS.seek 0ml
@20:59:21.99 delay 3 seconds
@20:59:25.01 AFV.select :carrierSolution
@20:59:25.12 delay 2 seconds
@20:59:27.14 AS.reconfigure ASconfig
@20:59:27.20 AS.seek 1ml
@21:00:24.94 delay 1 second
@21:00:25.96 AFV.select :holdingCoil
@21:00:26.07 delay 1 second
@21:00:27.09 AS.reconfigure ASfast
@21:00:27.15 AS.seek 0ml
@21:00:54.48 delay 3 seconds
@21:00:57.50 AFV.select :carrierSolution
@21:00:57.61 delay 2 seconds
@21:00:59.63 AS.reconfigure ASconfig
@21:00:59.69 AS.seek 1ml
@21:01:57.46 delay 1 second
@21:01:58.48 AFV.select :holdingCoil
@21:01:58.59 delay 1 second
@21:01:59.61 AS.reconfigure ASfast
@21:01:59.67 AS.seek 0ml
@21:02:27.01 delay 3 seconds
@21:02:30.03 AFV.select :carrierSolution
@21:02:30.14 delay 2 seconds
@21:02:32.16 AS.reconfigure ASconfig
@21:02:32.21 AS.seek 1ml
@21:03:29.96 delay 1 second
@21:03:30.98 AFV.select :holdingCoil
@21:03:31.09 delay 1 second
@21:03:32.11 AS.reconfigure ASnorm
@21:03:32.16 AS.seek 0ml
@21:04:22.88 Setting up PCR for AOPP1 -- writing /var/log/esp/AOPP121apr1521h.pcr
@21:04:22.89 Building PCR Slug from ASV with MM1 mix, PP1 primer
@21:04:22.92 Priming with carrier
@21:04:22.94 ATV.dial ARV
@21:04:23.58 ARV.dial :carrier
@21:04:23.93 AS.seek 0.5ml
@21:04:49.51 AFV.select :carrierSolution
@21:04:49.64 delay 2 seconds
@21:04:51.66 AS.reconfigure ASconfig
@21:04:51.73 AS.seek 1ml
@21:05:20.74 delay 1 second
@21:05:21.76 AFV.select :holdingCoil
@21:05:21.87 delay 1 second
@21:05:22.89 ATV.dial :waste
@21:05:24.67 AS.reconfigure ASnorm
@21:05:24.73 AS.seek 0ml
@21:06:15.44 AFV.select :carrierSolution
@21:06:15.56 delay 2 seconds
@21:06:17.58 AS.reconfigure ASconfig
@21:06:17.64 AS.seek 0.5ml
@21:06:46.68 delay 1 second
@21:06:47.70 AFV.select :holdingCoil
@21:06:47.81 Building Slug
@21:06:47.82 ATV.dial :ARV
@21:06:49.57 delay 2 seconds
@21:06:51.60 ARV.dial :air1
@21:06:52.00 delay 2 seconds
@21:06:54.07 AS.reconfigure ASslow2
@21:06:54.13 AS.seek 0.506ml
@21:07:00.38 delay 2 seconds
@21:07:02.41 ARV.dial :MM1
@21:07:03.04 delay 2 seconds
@21:07:05.07 Adding MM1
@21:07:05.14 AS.seek 0.524ml
@21:07:23.63 delay 25 seconds
@21:07:48.66 ARV.dial :PP1
@21:07:49.06 Adding PP1
@21:07:49.07 delay 2 seconds
@21:07:51.14 AS.seek 0.53ml
@21:07:57.63 delay 10 seconds
@21:08:07.67 ARV.dial :air1
@21:08:08.09 adding ARV air
@21:08:08.10 delay 2 seconds
@21:08:10.17 AS.seek 0.536ml
@21:08:16.49 delay 5 seconds
@21:08:21.52 ARV.dial :carrier
@21:08:21.94 delay 7 seconds
@21:08:29.01 AS.seek 0.594ml
@21:09:27.55 delay 5 seconds
@21:09:32.58 ATV.dial :ASV
@21:09:33.01 delay 2 seconds
@21:09:35.08 AS.seek 0.6ml
@21:09:41.55 delay 4 seconds
@21:09:45.58 ATV.dial ARV
@21:09:46.26 delay 2 seconds
@21:09:48.29 ARV.dial :carrier
@21:09:48.30 delay 2 seconds
@21:09:50.38 AS.seek 0.65ml
@21:10:41.12 delay 4 seconds
@21:10:45.15 ATV.dial PCR
@21:10:45.80 ASV.dial :waste1
@21:10:45.82 ARV.dial :air1
@21:10:46.28 Positioning Slug by 0.324ml
@21:10:46.36 AS.seek 0.326ml
@21:16:12.48 delay 5 seconds
@21:16:17.51 ATV.dial ARV
@21:16:18.32 ASV.dial :PCRlock
@21:16:19.15 delay 5 seconds
@21:16:24.18 Running AOPP1 Profile60CIQ1 thermocycling
@21:16:24.19 <PCR> PCR.program Profile60CIQ1
@21:16:24.32 Profile60CIQ1 should complete in < 1:56:15
@21:16:24.33 PCR.run 2:30:18.75
@22:55:29.25 PCR.off
@22:55:29.83 <MFBPCR> Running AOPP1 MeltProfile60 thermocycling
@22:55:29.85 <PCR> PCR.program MeltProfile60
@22:55:29.95 MeltProfile60 should complete in < 11:55
@22:55:29.96 PCR.run 19:53.75
@23:02:58.70 PCR.off
@23:03:00.03 <MFBPCR> PCR for AOPP1 completed -- closed /var/log/esp/AOPP121apr1521h.pcr
@23:03:00.11 Cleaning PCR
@23:03:00.12 AFV.select :carrierSolution
@23:03:00.24 delay 2 seconds
@23:03:02.26 AS.reconfigure ASconfig
@23:03:02.32 AS.seek 0.5ml
@23:03:12.60 delay 1 second
@23:03:13.62 AFV.select :holdingCoil
@23:03:13.73 ASV.dial :waste1
@23:03:14.12 ATV.dial :air3
@23:03:15.49 AS.reconfigure ASslow1
@23:03:15.55 AS.seek 0.51ml
@23:03:17.83 delay 2 seconds
@23:03:19.86 ATV.dial :bleach
@23:03:20.50 AS.seek 0.81ml
@23:04:21.07 delay 3 seconds
@23:04:24.10 ATV.dial :air3
@23:04:24.68 delay 3 seconds
@23:04:27.75 AS.seek 0.835ml
@23:04:33.13 delay 3 seconds
@23:04:36.16 ATV.dial :bleach
@23:04:36.80 AS.seek 0.82ml
@23:04:40.10 delay 3 seconds
@23:04:43.13 ARV.dial :waste
@23:04:43.76 delay 3 seconds
@23:04:46.79 ATV.dial :ARV
@23:04:47.72 AS.reconfigure ASconfig
@23:04:47.77 AS.seek 0.47ml
@23:05:08.18 AFV.select :carrierSolution
@23:05:08.30 delay 2 seconds
@23:05:10.32 AS.seek 1ml
@23:05:41.07 delay 1 second
@23:05:42.09 AFV.select :holdingCoil
@23:05:42.20 delay 1 second
@23:05:43.22 AS.reconfigure ASfast
@23:05:43.28 AS.seek 0ml
@23:06:10.62 AFV.select :carrierSolution
@23:06:10.74 delay 2 seconds
@23:06:12.76 AS.reconfigure ASconfig
@23:06:12.81 AS.seek 1ml
@23:07:10.57 delay 1 second
@23:07:11.59 AFV.select :holdingCoil
@23:07:11.70 delay 1 second
@23:07:12.72 AS.reconfigure ASfast
@23:07:12.77 AS.seek 0ml
@23:07:40.10 AFV.select :carrierSolution
@23:07:40.22 delay 2 seconds
@23:07:42.24 AS.reconfigure ASconfig
@23:07:42.29 AS.seek 1ml
@23:08:40.02 delay 1 second
@23:08:41.04 AFV.select :holdingCoil
@23:08:41.15 delay 1 second
@23:08:42.17 AS.reconfigure ASfast
@23:08:42.23 AS.seek 0ml
@23:09:09.54 ARV.dial between carrier and air1
@23:09:10.05 ATV.dial :air3
@23:09:11.43 AS.reconfigure ASslow1
@23:09:11.49 AS.seek 0.01ml
@23:09:13.90 delay 2 seconds
@23:09:15.93 ATV.dial :bleach
@23:09:16.57 AS.seek 0.81ml
@23:11:57.71 delay 3 seconds
@23:12:00.74 ATV.dial :air3
@23:12:01.32 delay 3 seconds
@23:12:04.39 AS.seek 0.835ml
@23:12:09.80 delay 3 seconds
@23:12:12.83 ATV.dial :bleach
@23:12:13.47 AS.seek 0.82ml
@23:12:16.77 delay 3 seconds
@23:12:19.80 ATV.dial PCR
@23:12:20.24 AS.reconfigure ASconfig
@23:12:20.29 AS.seek 0ml
@23:13:07.71 PCR.program :bleachProfile
@23:13:07.78 PCR program should complete in < 2:45
@23:13:07.79 PCR.run 10:00
@23:16:04.01 PCR.off
@23:16:04.06 AFV.select :carrierSolution
@23:16:04.17 delay 2 seconds
@23:16:06.19 AS.seek 1ml
@23:17:03.93 delay 1 second
@23:17:04.95 AFV.select :holdingCoil
@23:17:05.06 delay 1 second
@23:17:06.08 AS.reconfigure ASfast
@23:17:06.14 AS.seek 0ml
@23:17:33.48 delay 3 seconds
@23:17:36.50 AFV.select :carrierSolution
@23:17:36.61 delay 2 seconds
@23:17:38.63 AS.reconfigure ASconfig
@23:17:38.70 AS.seek 1ml
@23:18:36.44 delay 1 second
@23:18:37.46 AFV.select :holdingCoil
@23:18:37.57 delay 1 second
@23:18:38.59 AS.reconfigure ASfast
@23:18:38.65 AS.seek 0ml
@23:19:05.99 delay 3 seconds
@23:19:09.01 AFV.select :carrierSolution
@23:19:09.12 delay 2 seconds
@23:19:11.14 AS.reconfigure ASconfig
@23:19:11.19 AS.seek 1ml
@23:20:08.94 delay 1 second
@23:20:09.96 AFV.select :holdingCoil
@23:20:10.07 delay 1 second
@23:20:11.09 AS.reconfigure ASfast
@23:20:11.15 AS.seek 0ml
@23:20:38.49 delay 3 seconds
@23:20:41.51 AFV.select :carrierSolution
@23:20:41.62 delay 2 seconds
@23:20:43.64 AS.reconfigure ASconfig
@23:20:43.70 AS.seek 1ml
@23:21:41.48 delay 1 second
@23:21:42.50 AFV.select :holdingCoil
@23:21:42.61 delay 1 second
@23:21:43.63 AS.reconfigure ASfast
@23:21:43.68 AS.seek 0ml
@23:22:11.01 delay 3 seconds
@23:22:14.03 AFV.select :carrierSolution
@23:22:14.14 delay 2 seconds
@23:22:16.16 AS.reconfigure ASconfig
@23:22:16.21 AS.seek 1ml
@23:23:13.96 delay 1 second
@23:23:14.98 AFV.select :holdingCoil
@23:23:15.09 delay 1 second
@23:23:16.11 AS.reconfigure ASnorm
@23:23:16.16 AS.seek 0ml
@23:24:06.85 Setting up PCR for AARPP4 -- writing /var/log/esp/AARPP421apr1523h.pcr
@23:24:06.86 Building PCR Slug from ASV with MM2 mix, PP4 primer
@23:24:06.89 Priming with carrier
@23:24:06.90 ATV.dial ARV
@23:24:07.55 ARV.dial :carrier
@23:24:07.88 AS.seek 0.5ml
@23:24:33.44 AFV.select :carrierSolution
@23:24:33.56 delay 2 seconds
@23:24:35.58 AS.reconfigure ASconfig
@23:24:35.64 AS.seek 1ml
@23:25:04.63 delay 1 second
@23:25:05.65 AFV.select :holdingCoil
@23:25:05.76 delay 1 second
@23:25:06.78 ATV.dial :waste
@23:25:08.58 AS.reconfigure ASnorm
@23:25:08.64 AS.seek 0ml
@23:25:59.33 AFV.select :carrierSolution
@23:25:59.45 delay 2 seconds
@23:26:01.47 AS.reconfigure ASconfig
@23:26:01.53 AS.seek 0.5ml
@23:26:30.55 delay 1 second
@23:26:31.57 AFV.select :holdingCoil
@23:26:31.68 Building Slug
@23:26:31.69 ATV.dial :ARV
@23:26:33.43 delay 2 seconds
@23:26:35.46 ARV.dial :air1
@23:26:35.86 delay 2 seconds
@23:26:37.93 AS.reconfigure ASslow2
@23:26:37.99 AS.seek 0.506ml
@23:26:44.38 delay 2 seconds
@23:26:46.41 ARV.dial :MM2
@23:26:47.99 delay 2 seconds
@23:26:50.02 Adding MM2
@23:26:50.10 AS.seek 0.524ml
@23:27:08.71 delay 25 seconds
@23:27:33.74 ARV.dial :PP4
@23:27:34.15 Adding PP4
@23:27:34.17 delay 2 seconds
@23:27:36.24 AS.seek 0.53ml
@23:27:42.66 delay 10 seconds
@23:27:52.70 ARV.dial :air3
@23:27:53.12 adding ARV air
@23:27:53.14 delay 2 seconds
@23:27:55.20 AS.seek 0.536ml
@23:28:01.46 delay 5 seconds
@23:28:06.49 ARV.dial :carrier
@23:28:07.80 delay 7 seconds
@23:28:14.88 AS.seek 0.594ml
@23:29:13.33 delay 5 seconds
@23:29:18.36 ATV.dial :ASV
@23:29:18.80 delay 2 seconds
@23:29:21.29 AS.seek 0.6ml
@23:29:27.48 delay 4 seconds
@23:29:31.51 ATV.dial ARV
@23:29:32.10 delay 2 seconds
@23:29:34.13 ARV.dial :carrier
@23:29:34.14 delay 2 seconds
@23:29:36.22 AS.seek 0.65ml
@23:30:26.73 delay 4 seconds
@23:30:30.76 ATV.dial PCR
@23:30:31.43 ASV.dial :waste1
@23:30:31.45 ARV.dial :air1
@23:30:31.86 Positioning Slug by 0.324ml
@23:30:31.95 AS.seek 0.326ml
@23:35:58.15 delay 5 seconds
@23:36:03.18 ATV.dial ARV
@23:36:03.99 ASV.dial :PCRlock
@23:36:04.40 delay 5 seconds
@23:36:09.43 Running AARPP4 Profile58CIQ2 thermocycling
@23:36:09.44 <PCR> PCR.program Profile58CIQ2
@23:36:09.56 Profile58CIQ2 should complete in < 1:36:15
@23:36:09.57 PCR.run 2:05:18.75
@00:57:01.29CEST16-Apr-21 PCR.off
@00:57:01.86 <MFBPCR> Running AARPP4 MeltProfile58 thermocycling
@00:57:01.88 <PCR> PCR.program MeltProfile58
@00:57:01.98 MeltProfile58 should complete in < 12:33
@00:57:01.99 PCR.run 20:41.25
@01:04:37.72 PCR.off
@01:04:38.71 <MFBPCR> PCR for AARPP4 completed -- closed /var/log/esp/AARPP421apr1523h.pcr
@01:04:38.78 Cleaning PCR
@01:04:38.80 AFV.select :carrierSolution
@01:04:38.92 delay 2 seconds
@01:04:40.94 AS.reconfigure ASconfig
@01:04:40.99 AS.seek 0.5ml
@01:04:51.32 delay 1 second
@01:04:52.34 AFV.select :holdingCoil
@01:04:52.45 ASV.dial :waste1
@01:04:52.85 ATV.dial :air3
@01:04:54.22 AS.reconfigure ASslow1
@01:04:54.28 AS.seek 0.51ml
@01:04:56.55 delay 2 seconds
@01:04:58.58 ATV.dial :bleach
@01:04:59.22 AS.seek 0.81ml
@01:05:59.80 delay 3 seconds
@01:06:02.83 ATV.dial :air3
@01:06:03.41 delay 3 seconds
@01:06:06.48 AS.seek 0.835ml
@01:06:11.78 delay 3 seconds
@01:06:14.81 ATV.dial :bleach
@01:06:15.44 AS.seek 0.82ml
@01:06:18.85 delay 3 seconds
@01:06:21.88 ARV.dial :waste
@01:06:22.51 delay 3 seconds
@01:06:25.54 ATV.dial :ARV
@01:06:26.47 AS.reconfigure ASconfig
@01:06:26.52 AS.seek 0.47ml
@01:06:46.93 AFV.select :carrierSolution
@01:06:47.05 delay 2 seconds
@01:06:49.07 AS.seek 1ml
@01:07:19.80 delay 1 second
@01:07:20.82 AFV.select :holdingCoil
@01:07:20.93 delay 1 second
@01:07:21.95 AS.reconfigure ASfast
@01:07:22.00 AS.seek 0ml
@01:07:49.34 AFV.select :carrierSolution
@01:07:49.46 delay 2 seconds
@01:07:51.48 AS.reconfigure ASconfig
@01:07:51.53 AS.seek 1ml
@01:08:49.29 delay 1 second
@01:08:50.31 AFV.select :holdingCoil
@01:08:50.42 delay 1 second
@01:08:51.44 AS.reconfigure ASfast
@01:08:51.49 AS.seek 0ml
@01:09:18.85 AFV.select :carrierSolution
@01:09:18.97 delay 2 seconds
@01:09:20.99 AS.reconfigure ASconfig
@01:09:21.04 AS.seek 1ml
@01:10:18.84 delay 1 second
@01:10:19.86 AFV.select :holdingCoil
@01:10:19.97 delay 1 second
@01:10:20.99 AS.reconfigure ASfast
@01:10:21.05 AS.seek 0ml
@01:10:48.41 ARV.dial between carrier and air1
@01:10:48.92 ATV.dial :air3
@01:10:50.29 AS.reconfigure ASslow1
@01:10:50.35 AS.seek 0.01ml
@01:10:52.76 delay 2 seconds
@01:10:54.79 ATV.dial :bleach
@01:10:55.44 AS.seek 0.81ml
@01:13:36.65 delay 3 seconds
@01:13:39.68 ATV.dial :air3
@01:13:40.26 delay 3 seconds
@01:13:43.33 AS.seek 0.835ml
@01:13:48.74 delay 3 seconds
@01:13:51.77 ATV.dial :bleach
@01:13:52.41 AS.seek 0.82ml
@01:13:55.70 delay 3 seconds
@01:13:58.73 ATV.dial PCR
@01:13:59.16 AS.reconfigure ASconfig
@01:13:59.21 AS.seek 0ml
@01:14:47.02 PCR.program :bleachProfile
@01:14:47.10 PCR program should complete in < 2:45
@01:14:47.11 PCR.run 10:00
@01:17:42.89 PCR.off
@01:17:42.93 AFV.select :carrierSolution
@01:17:43.04 delay 2 seconds
@01:17:45.06 AS.seek 1ml
@01:18:42.82 delay 1 second
@01:18:43.84 AFV.select :holdingCoil
@01:18:43.95 delay 1 second
@01:18:44.97 AS.reconfigure ASfast
@01:18:45.02 AS.seek 0ml
@01:19:12.37 delay 3 seconds
@01:19:15.39 AFV.select :carrierSolution
@01:19:15.50 delay 2 seconds
@01:19:17.52 AS.reconfigure ASconfig
@01:19:17.58 AS.seek 1ml
@01:20:15.35 delay 1 second
@01:20:16.37 AFV.select :holdingCoil
@01:20:16.48 delay 1 second
@01:20:17.50 AS.reconfigure ASfast
@01:20:17.55 AS.seek 0ml
@01:20:44.88 delay 3 seconds
@01:20:47.90 AFV.select :carrierSolution
@01:20:48.01 delay 2 seconds
@01:20:50.03 AS.reconfigure ASconfig
@01:20:50.08 AS.seek 1ml
@01:21:47.87 delay 1 second
@01:21:48.89 AFV.select :holdingCoil
@01:21:49.00 delay 1 second
@01:21:50.02 AS.reconfigure ASfast
@01:21:50.07 AS.seek 0ml
@01:22:17.41 delay 3 seconds
@01:22:20.43 AFV.select :carrierSolution
@01:22:20.54 delay 2 seconds
@01:22:22.56 AS.reconfigure ASconfig
@01:22:22.63 AS.seek 1ml
@01:23:20.38 delay 1 second
@01:23:21.40 AFV.select :holdingCoil
@01:23:21.51 delay 1 second
@01:23:22.53 AS.reconfigure ASfast
@01:23:22.58 AS.seek 0ml
@01:23:49.93 delay 3 seconds
@01:23:52.95 AFV.select :carrierSolution
@01:23:53.06 delay 2 seconds
@01:23:55.08 AS.reconfigure ASconfig
@01:23:55.14 AS.seek 1ml
@01:24:52.90 delay 1 second
@01:24:53.92 AFV.select :holdingCoil
@01:24:54.03 delay 1 second
@01:24:55.05 AS.reconfigure ASnorm
@01:24:55.11 AS.seek 0ml
@01:25:45.83 Setting up PCR for AALPP3 -- writing /var/log/esp/AALPP321apr1601h.pcr
@01:25:45.84 Building PCR Slug from ASV with MM2 mix, PP3 primer
@01:25:45.87 Priming with carrier
@01:25:45.88 ATV.dial ARV
@01:25:46.54 ARV.dial :carrier
@01:25:46.90 AS.seek 0.5ml
@01:26:12.54 AFV.select :carrierSolution
@01:26:12.66 delay 2 seconds
@01:26:14.68 AS.reconfigure ASconfig
@01:26:14.74 AS.seek 1ml
@01:26:43.74 delay 1 second
@01:26:44.77 AFV.select :holdingCoil
@01:26:44.87 delay 1 second
@01:26:45.89 ATV.dial :waste
@01:26:47.69 AS.reconfigure ASnorm
@01:26:47.75 AS.seek 0ml
@01:27:38.46 AFV.select :carrierSolution
@01:27:38.58 delay 2 seconds
@01:27:41.01 AS.reconfigure ASconfig
@01:27:41.07 AS.seek 0.5ml
@01:28:10.10 delay 1 second
@01:28:11.12 AFV.select :holdingCoil
@01:28:11.23 Building Slug
@01:28:11.24 ATV.dial :ARV
@01:28:12.99 delay 2 seconds
@01:28:15.02 ARV.dial :air1
@01:28:15.42 delay 2 seconds
@01:28:17.49 AS.reconfigure ASslow2
@01:28:17.55 AS.seek 0.506ml
@01:28:23.96 delay 2 seconds
@01:28:25.99 ARV.dial :MM2
@01:28:27.57 delay 2 seconds
@01:28:29.60 Adding MM2
@01:28:29.67 AS.seek 0.524ml
@01:28:48.16 delay 25 seconds
@01:29:13.19 ARV.dial :PP3
@01:29:13.61 Adding PP3
@01:29:13.62 delay 2 seconds
@01:29:15.69 AS.seek 0.53ml
@01:29:22.05 delay 10 seconds
@01:29:32.09 ARV.dial :air2
@01:29:32.50 adding ARV air
@01:29:32.51 delay 2 seconds
@01:29:34.58 AS.seek 0.536ml
@01:29:40.80 delay 5 seconds
@01:29:45.83 ARV.dial :carrier
@01:29:47.18 delay 7 seconds
@01:29:54.26 AS.seek 0.594ml
@01:30:52.74 delay 5 seconds
@01:30:57.77 ATV.dial :ASV
@01:30:58.21 delay 2 seconds
@01:31:00.28 AS.seek 0.6ml
@01:31:06.49 delay 4 seconds
@01:31:10.52 ATV.dial ARV
@01:31:11.10 delay 2 seconds
@01:31:13.13 ARV.dial :carrier
@01:31:13.14 delay 2 seconds
@01:31:15.21 AS.seek 0.65ml
@01:32:05.73 delay 4 seconds
@01:32:09.76 ATV.dial PCR
@01:32:10.41 ASV.dial :waste1
@01:32:10.43 ARV.dial :air1
@01:32:10.85 Positioning Slug by 0.324ml
@01:32:10.94 AS.seek 0.326ml
@01:37:37.12 delay 5 seconds
@01:37:42.15 ATV.dial ARV
@01:37:42.96 ASV.dial :PCRlock
@01:37:43.37 delay 5 seconds
@01:37:48.40 Running AALPP3 Profile58CIQ2 thermocycling
@01:37:48.41 <PCR> PCR.program Profile58CIQ2
@01:37:48.53 Profile58CIQ2 should complete in < 1:36:15
@01:37:48.54 PCR.run 2:05:18.75
@02:58:39.24 PCR.off
@02:58:39.82 <MFBPCR> Running AALPP3 MeltProfile58 thermocycling
@02:58:39.84 <PCR> PCR.program MeltProfile58
@02:58:39.95 MeltProfile58 should complete in < 12:33
@02:58:39.96 PCR.run 20:41.25
@03:06:27.61 PCR.off
@03:06:28.61 <MFBPCR> PCR for AALPP3 completed -- closed /var/log/esp/AALPP321apr1601h.pcr
@03:06:28.68 Cleaning PCR
@03:06:28.69 AFV.select :carrierSolution
@03:06:28.82 delay 2 seconds
@03:06:30.84 AS.reconfigure ASconfig
@03:06:30.89 AS.seek 0.5ml
@03:06:41.21 delay 1 second
@03:06:42.23 AFV.select :holdingCoil
@03:06:42.34 ASV.dial :waste1
@03:06:42.73 ATV.dial :air3
@03:06:44.10 AS.reconfigure ASslow1
@03:06:44.15 AS.seek 0.51ml
@03:06:46.53 delay 2 seconds
@03:06:48.56 ATV.dial :bleach
@03:06:49.19 AS.seek 0.81ml
@03:07:49.79 delay 3 seconds
@03:07:52.82 ATV.dial :air3
@03:07:53.40 delay 3 seconds
@03:07:56.47 AS.seek 0.835ml
@03:08:01.78 delay 3 seconds
@03:08:04.81 ATV.dial :bleach
@03:08:05.44 AS.seek 0.82ml
@03:08:08.87 delay 3 seconds
@03:08:11.90 ARV.dial :waste
@03:08:12.52 delay 3 seconds
@03:08:15.55 ATV.dial :ARV
@03:08:16.49 AS.reconfigure ASconfig
@03:08:16.54 AS.seek 0.47ml
@03:08:36.98 AFV.select :carrierSolution
@03:08:37.10 delay 2 seconds
@03:08:39.12 AS.seek 1ml
@03:09:09.87 delay 1 second
@03:09:10.89 AFV.select :holdingCoil
@03:09:11.06 delay 1 second
@03:09:12.08 AS.reconfigure ASfast
@03:09:12.14 AS.seek 0ml
@03:09:39.45 AFV.select :carrierSolution
@03:09:39.57 delay 2 seconds
@03:09:41.59 AS.reconfigure ASconfig
@03:09:41.64 AS.seek 1ml
@03:10:39.40 delay 1 second
@03:10:40.42 AFV.select :holdingCoil
@03:10:40.53 delay 1 second
@03:10:41.55 AS.reconfigure ASfast
@03:10:41.61 AS.seek 0ml
@03:11:08.95 AFV.select :carrierSolution
@03:11:09.07 delay 2 seconds
@03:11:11.09 AS.reconfigure ASconfig
@03:11:11.14 AS.seek 1ml
@03:12:08.88 delay 1 second
@03:12:09.90 AFV.select :holdingCoil
@03:12:10.01 delay 1 second
@03:12:11.03 AS.reconfigure ASfast
@03:12:11.09 AS.seek 0ml
@03:12:38.43 ARV.dial between carrier and air1
@03:12:38.97 ATV.dial :air3
@03:12:40.33 AS.reconfigure ASslow1
@03:12:40.39 AS.seek 0.01ml
@03:12:42.84 delay 2 seconds
@03:12:44.87 ATV.dial :bleach
@03:12:45.52 AS.seek 0.81ml
@03:15:26.77 delay 3 seconds
@03:15:29.80 ATV.dial :air3
@03:15:30.40 delay 3 seconds
@03:15:33.47 AS.seek 0.835ml
@03:15:38.78 delay 3 seconds
@03:15:41.81 ATV.dial :bleach
@03:15:42.46 AS.seek 0.82ml
@03:15:45.79 delay 3 seconds
@03:15:48.82 ATV.dial PCR
@03:15:49.25 AS.reconfigure ASconfig
@03:15:49.30 AS.seek 0ml
@03:16:36.71 PCR.program :bleachProfile
@03:16:36.78 PCR program should complete in < 2:45
@03:16:36.79 PCR.run 10:00
@03:19:32.58 PCR.off
@03:19:32.62 AFV.select :carrierSolution
@03:19:32.73 delay 2 seconds
@03:19:34.75 AS.seek 1ml
@03:20:32.52 delay 1 second
@03:20:33.54 AFV.select :holdingCoil
@03:20:33.65 delay 1 second
@03:20:34.67 AS.reconfigure ASfast
@03:20:34.73 AS.seek 0ml
@03:21:02.07 delay 3 seconds
@03:21:05.09 AFV.select :carrierSolution
@03:21:05.20 delay 2 seconds
@03:21:07.22 AS.reconfigure ASconfig
@03:21:07.28 AS.seek 1ml
@03:22:05.01 delay 1 second
@03:22:06.03 AFV.select :holdingCoil
@03:22:06.14 delay 1 second
@03:22:07.16 AS.reconfigure ASfast
@03:22:07.21 AS.seek 0ml
@03:22:34.56 delay 3 seconds
@03:22:37.58 AFV.select :carrierSolution
@03:22:37.69 delay 2 seconds
@03:22:40.11 AS.reconfigure ASconfig
@03:22:40.19 AS.seek 1ml
@03:23:37.96 delay 1 second
@03:23:38.98 AFV.select :holdingCoil
@03:23:39.09 delay 1 second
@03:23:40.11 AS.reconfigure ASfast
@03:23:40.17 AS.seek 0ml
@03:24:07.51 delay 3 seconds
@03:24:10.53 AFV.select :carrierSolution
@03:24:10.64 delay 2 seconds
@03:24:12.66 AS.reconfigure ASconfig
@03:24:12.73 AS.seek 1ml
@03:25:10.49 delay 1 second
@03:25:11.51 AFV.select :holdingCoil
@03:25:11.62 delay 1 second
@03:25:12.64 AS.reconfigure ASfast
@03:25:12.70 AS.seek 0ml
@03:25:40.02 delay 3 seconds
@03:25:43.04 AFV.select :carrierSolution
@03:25:43.15 delay 2 seconds
@03:25:45.17 AS.reconfigure ASconfig
@03:25:45.23 AS.seek 1ml
@03:26:42.99 delay 1 second
@03:26:44.01 AFV.select :holdingCoil
@03:26:44.12 delay 1 second
@03:26:45.14 AS.reconfigure ASnorm
@03:26:45.19 AS.seek 0ml
@03:27:35.96 Setting up PCR for ACOPP5 -- writing /var/log/esp/ACOPP521apr1603h.pcr
@03:27:35.98 Building PCR Slug from ASV with MM3 mix, PP5 primer
@03:27:36.00 Priming with carrier
@03:27:36.02 ATV.dial ARV
@03:27:36.66 ARV.dial :carrier
@03:27:37.01 AS.seek 0.5ml
@03:28:02.54 AFV.select :carrierSolution
@03:28:02.67 delay 2 seconds
@03:28:04.69 AS.reconfigure ASconfig
@03:28:04.76 AS.seek 1ml
@03:28:33.79 delay 1 second
@03:28:34.82 AFV.select :holdingCoil
@03:28:34.92 delay 1 second
@03:28:35.94 ATV.dial :waste
@03:28:37.74 AS.reconfigure ASnorm
@03:28:37.80 AS.seek 0ml
@03:29:28.52 AFV.select :carrierSolution
@03:29:28.65 delay 2 seconds
@03:29:30.67 AS.reconfigure ASconfig
@03:29:30.73 AS.seek 0.5ml
@03:29:59.77 delay 1 second
@03:30:00.79 AFV.select :holdingCoil
@03:30:00.90 Building Slug
@03:30:00.91 ATV.dial :ARV
@03:30:02.66 delay 2 seconds
@03:30:04.69 ARV.dial :air1
@03:30:05.09 delay 2 seconds
@03:30:07.16 AS.reconfigure ASslow2
@03:30:07.22 AS.seek 0.506ml
@03:30:13.41 delay 2 seconds
@03:30:15.44 ARV.dial :MM3
@03:30:16.54 delay 2 seconds
@03:30:18.57 Adding MM3
@03:30:18.65 AS.seek 0.524ml
@03:30:36.88 delay 25 seconds
@03:31:01.91 ARV.dial :PP5
@03:31:02.32 Adding PP5
@03:31:02.34 delay 2 seconds
@03:31:04.41 AS.seek 0.53ml
@03:31:10.62 delay 10 seconds
@03:31:20.66 ARV.dial :air3
@03:31:21.06 adding ARV air
@03:31:21.08 delay 2 seconds
@03:31:23.15 AS.seek 0.536ml
@03:31:29.65 delay 5 seconds
@03:31:34.68 ARV.dial :carrier
@03:31:36.01 delay 7 seconds
@03:31:43.08 AS.seek 0.594ml
@03:32:41.62 delay 5 seconds
@03:32:46.65 ATV.dial :ASV
@03:32:47.07 delay 2 seconds
@03:32:49.14 AS.seek 0.6ml
@03:32:55.82 delay 4 seconds
@03:32:59.85 ATV.dial ARV
@03:33:00.43 delay 2 seconds
@03:33:02.46 ARV.dial :carrier
@03:33:02.47 delay 2 seconds
@03:33:04.54 AS.seek 0.65ml
@03:33:55.59 delay 4 seconds
@03:33:59.62 ATV.dial PCR
@03:34:00.26 ASV.dial :waste1
@03:34:00.28 ARV.dial :air1
@03:34:00.71 Positioning Slug by 0.324ml
@03:34:00.80 AS.seek 0.326ml
@03:39:27.12 delay 5 seconds
@03:39:32.15 ATV.dial ARV
@03:39:32.96 ASV.dial :PCRlock
@03:39:33.37 delay 5 seconds
@03:39:38.40 Running ACOPP5 ProfileIPc thermocycling
@03:39:38.41 <PCR> PCR.program ProfileIPc
@03:39:38.53 ProfileIPc should complete in < 1:26:35
@03:39:38.54 PCR.run 1:53:13.75
@04:48:21.62 PCR.off
@04:48:22.19 <MFBPCR> PCR for ACOPP5 completed -- closed /var/log/esp/ACOPP521apr1603h.pcr
@04:48:22.26 Cleaning PCR
@04:48:22.27 AFV.select :carrierSolution
@04:48:22.39 delay 2 seconds
@04:48:24.41 AS.reconfigure ASconfig
@04:48:24.47 AS.seek 0.5ml
@04:48:34.76 delay 1 second
@04:48:35.78 AFV.select :holdingCoil
@04:48:35.89 ASV.dial :waste1
@04:48:36.29 ATV.dial :air3
@04:48:37.66 AS.reconfigure ASslow1
@04:48:37.72 AS.seek 0.51ml
@04:48:40.00 delay 2 seconds
@04:48:42.03 ATV.dial :bleach
@04:48:42.66 AS.seek 0.81ml
@04:49:43.24 delay 3 seconds
@04:49:46.27 ATV.dial :air3
@04:49:46.85 delay 3 seconds
@04:49:49.92 AS.seek 0.835ml
@04:49:55.21 delay 3 seconds
@04:49:58.24 ATV.dial :bleach
@04:49:59.27 AS.seek 0.82ml
@04:50:02.56 delay 3 seconds
@04:50:05.59 ARV.dial :waste
@04:50:06.21 delay 3 seconds
@04:50:09.24 ATV.dial :ARV
@04:50:10.18 AS.reconfigure ASconfig
@04:50:10.24 AS.seek 0.47ml
@04:50:30.63 AFV.select :carrierSolution
@04:50:30.75 delay 2 seconds
@04:50:32.77 AS.seek 1ml
@04:51:03.52 delay 1 second
@04:51:04.54 AFV.select :holdingCoil
@04:51:04.65 delay 1 second
@04:51:05.67 AS.reconfigure ASfast
@04:51:05.73 AS.seek 0ml
@04:51:33.07 AFV.select :carrierSolution
@04:51:33.19 delay 2 seconds
@04:51:35.21 AS.reconfigure ASconfig
@04:51:35.27 AS.seek 1ml
@04:52:33.02 delay 1 second
@04:52:34.04 AFV.select :holdingCoil
@04:52:34.15 delay 1 second
@04:52:35.17 AS.reconfigure ASfast
@04:52:35.23 AS.seek 0ml
@04:53:02.54 AFV.select :carrierSolution
@04:53:02.66 delay 2 seconds
@04:53:04.68 AS.reconfigure ASconfig
@04:53:04.74 AS.seek 1ml
@04:54:02.48 delay 1 second
@04:54:03.50 AFV.select :holdingCoil
@04:54:03.61 delay 1 second
@04:54:04.63 AS.reconfigure ASfast
@04:54:04.69 AS.seek 0ml
@04:54:32.02 ARV.dial between carrier and air1
@04:54:32.57 ATV.dial :air3
@04:54:33.93 AS.reconfigure ASslow1
@04:54:33.99 AS.seek 0.01ml
@04:54:36.38 delay 2 seconds
@04:54:38.41 ATV.dial :bleach
@04:54:39.07 AS.seek 0.81ml
@04:57:20.20 delay 3 seconds
@04:57:23.23 ATV.dial :air3
@04:57:23.82 delay 3 seconds
@04:57:26.89 AS.seek 0.835ml
@04:57:32.17 delay 3 seconds
@04:57:35.20 ATV.dial :bleach
@04:57:35.85 AS.seek 0.82ml
@04:57:39.17 delay 3 seconds
@04:57:42.20 ATV.dial PCR
@04:57:42.63 AS.reconfigure ASconfig
@04:57:42.68 AS.seek 0ml
@04:58:30.12 PCR.program :bleachProfile
@04:58:30.20 PCR program should complete in < 2:45
@04:58:30.21 PCR.run 10:00
@05:01:26.00 PCR.off
@05:01:26.05 AFV.select :carrierSolution
@05:01:26.16 delay 2 seconds
@05:01:28.18 AS.seek 1ml
@05:02:25.96 delay 1 second
@05:02:26.98 AFV.select :holdingCoil
@05:02:27.51 delay 1 second
@05:02:28.53 AS.reconfigure ASfast
@05:02:28.59 AS.seek 0ml
@05:02:55.93 delay 3 seconds
@05:02:58.95 AFV.select :carrierSolution
@05:02:59.06 delay 2 seconds
@05:03:01.08 AS.reconfigure ASconfig
@05:03:01.14 AS.seek 1ml
@05:03:58.88 delay 1 second
@05:03:59.90 AFV.select :holdingCoil
@05:04:00.01 delay 1 second
@05:04:01.03 AS.reconfigure ASfast
@05:04:01.09 AS.seek 0ml
@05:04:28.43 delay 3 seconds
@05:04:31.46 AFV.select :carrierSolution
@05:04:31.56 delay 2 seconds
@05:04:33.58 AS.reconfigure ASconfig
@05:04:33.65 AS.seek 1ml
@05:05:31.40 delay 1 second
@05:05:32.42 AFV.select :holdingCoil
@05:05:32.53 delay 1 second
@05:05:33.55 AS.reconfigure ASfast
@05:05:33.61 AS.seek 0ml
@05:06:00.95 delay 3 seconds
@05:06:03.97 AFV.select :carrierSolution
@05:06:04.08 delay 2 seconds
@05:06:06.10 AS.reconfigure ASconfig
@05:06:06.15 AS.seek 1ml
@05:07:03.88 delay 1 second
@05:07:04.90 AFV.select :holdingCoil
@05:07:05.01 delay 1 second
@05:07:06.03 AS.reconfigure ASfast
@05:07:06.09 AS.seek 0ml
@05:07:33.43 delay 3 seconds
@05:07:36.45 AFV.select :carrierSolution
@05:07:36.56 delay 2 seconds
@05:07:38.58 AS.reconfigure ASconfig
@05:07:38.65 AS.seek 1ml
@05:08:36.40 delay 1 second
@05:08:37.42 AFV.select :holdingCoil
@05:08:37.53 delay 1 second
@05:08:38.55 AS.reconfigure ASnorm
@05:08:38.60 AS.seek 0ml
@05:09:29.30 Setting up PCR for AIPc -- writing /var/log/esp/AIPc21apr1605h.pcr
@05:09:29.31 Building PCR Slug from ASV with MM3 mix, PP6 primer
@05:09:29.34 Priming with carrier
@05:09:29.35 ATV.dial ARV
@05:09:30.01 ARV.dial :carrier
@05:09:30.37 AS.seek 0.5ml
@05:09:55.96 AFV.select :carrierSolution
@05:09:56.08 delay 2 seconds
@05:09:58.10 AS.reconfigure ASconfig
@05:09:58.16 AS.seek 1ml
@05:10:27.17 delay 1 second
@05:10:28.20 AFV.select :holdingCoil
@05:10:28.30 delay 1 second
@05:10:29.32 ATV.dial :waste
@05:10:31.11 AS.reconfigure ASnorm
@05:10:31.17 AS.seek 0ml
@05:11:21.84 AFV.select :carrierSolution
@05:11:21.97 delay 2 seconds
@05:11:23.99 AS.reconfigure ASconfig
@05:11:24.05 AS.seek 0.5ml
@05:11:53.10 delay 1 second
@05:11:54.12 AFV.select :holdingCoil
@05:11:54.23 Building Slug
@05:11:54.24 ATV.dial :ARV
@05:11:55.98 delay 2 seconds
@05:11:58.01 ARV.dial :air1
@05:11:58.41 delay 2 seconds
@05:12:00.48 AS.reconfigure ASslow2
@05:12:00.54 AS.seek 0.506ml
@05:12:07.12 delay 2 seconds
@05:12:09.15 ARV.dial :MM3
@05:12:10.24 delay 2 seconds
@05:12:12.27 Adding MM3
@05:12:12.34 AS.seek 0.524ml
@05:12:30.85 delay 25 seconds
@05:12:55.88 ARV.dial :PP6
@05:12:56.27 Adding PP6
@05:12:56.29 delay 2 seconds
@05:12:58.36 AS.seek 0.53ml
@05:13:05.02 delay 10 seconds
@05:13:15.06 ARV.dial :air1
@05:13:15.93 adding ARV air
@05:13:15.95 delay 2 seconds
@05:13:18.02 AS.seek 0.536ml
@05:13:24.31 delay 5 seconds
@05:13:29.34 ARV.dial :carrier
@05:13:29.74 delay 7 seconds
@05:13:36.82 AS.seek 0.594ml
@05:14:35.27 delay 5 seconds
@05:14:40.30 ATV.dial :ASV
@05:14:40.73 delay 2 seconds
@05:14:42.80 AS.seek 0.6ml
@05:14:49.45 delay 4 seconds
@05:14:53.48 ATV.dial ARV
@05:14:54.15 delay 2 seconds
@05:14:56.18 ARV.dial :carrier
@05:14:56.19 delay 2 seconds
@05:14:58.26 AS.seek 0.65ml
@05:15:48.71 delay 4 seconds
@05:15:52.74 ATV.dial PCR
@05:15:53.38 ASV.dial :waste1
@05:15:53.40 ARV.dial :air1
@05:15:53.83 Positioning Slug by 0.324ml
@05:15:53.91 AS.seek 0.326ml
@05:21:19.99 delay 5 seconds
@05:21:25.02 ATV.dial ARV
@05:21:25.82 ASV.dial :PCRlock
@05:21:26.23 delay 5 seconds
@05:21:31.26 Running AIPc ProfileIPc thermocycling
@05:21:31.27 <PCR> PCR.program ProfileIPc
@05:21:31.39 ProfileIPc should complete in < 1:26:35
@05:21:31.40 PCR.run 1:53:13.75
@06:30:02.81 PCR.off
@06:30:03.38 <MFBPCR> PCR for AIPc completed -- closed /var/log/esp/AIPc21apr1605h.pcr
@06:30:03.40 Full MFB Decontamination
@06:30:03.42 Decon extraction column and decon extSrc
@06:30:03.45 SPE.hold 2:46:40,55C
@06:30:03.49 ASV.dial :SPEdelivery
@06:30:04.17 delay 20 seconds
@06:30:24.20 AFV.select :carrierSolution
@06:30:24.31 delay 2 seconds
@06:30:26.33 AS.reconfigure ASconfig
@06:30:26.38 AS.seek 0.5ml
@06:30:36.68 delay 1 second
@06:30:37.70 AFV.select :holdingCoil
@06:30:37.81 ATV.dial :air1
@06:30:40.07 AS.reconfigure ASslow1
@06:30:40.13 AS.seek 0.52ml
@06:30:44.85 ATV.dial :ASV
@06:30:46.60 ASV.dial :SPErecovery
@06:30:47.30 AS.seek 0.62ml
@06:31:07.71 ATV.dial :waste
@06:31:09.91 AS.reconfigure ASnorm
@06:31:09.97 AS.seek 0ml
@06:31:41.54 deconEluateRecoveryLine extSrc
@06:31:41.55 ATV.dial :waste
@06:31:41.59 AS.seek 0ml
@06:31:41.67 ATV.dial :air3
@06:31:42.45 AS.reconfigure ASslow1
@06:31:42.51 AS.seek 0.01ml
@06:31:44.96 delay 2 seconds
@06:31:46.99 ATV.dial :bleach
@06:31:47.49 AS.seek 0.31ml
@06:32:48.34 delay 3 seconds
@06:32:51.37 ATV.dial :air3
@06:32:51.99 delay 3 seconds
@06:32:55.07 AS.seek 0.335ml
@06:33:00.40 delay 3 seconds
@06:33:03.43 ATV.dial :bleach
@06:33:04.10 AS.seek 0.32ml
@06:33:07.42 delay 3 seconds
@06:33:10.45 ATV.dial :extSrc
@06:33:11.15 AS.seek 0ml
@06:34:15.90 AFV.select :carrierSolution
@06:34:16.02 delay 2 seconds
@06:34:18.04 AS.reconfigure ASconfig
@06:34:18.10 AS.seek 0.5ml
@06:34:47.12 delay 1 second
@06:34:48.14 AFV.select :holdingCoil
@06:34:48.25 ATV.dial :extSrc
@06:34:48.27 AS.reconfigure ASslow1
@06:34:48.33 AS.seek 0ml
@06:36:29.15 AFV.select :carrierSolution
@06:36:29.27 delay 2 seconds
@06:36:31.29 AS.reconfigure ASconfig
@06:36:31.35 AS.seek 0.5ml
@06:37:00.39 delay 1 second
@06:37:01.41 AFV.select :holdingCoil
@06:37:01.52 ATV.dial :extSrc
@06:37:01.54 AS.reconfigure ASslow1
@06:37:01.61 AS.seek 0ml
@06:38:42.45 AFV.select :carrierSolution
@06:38:42.57 delay 2 seconds
@06:38:44.59 AS.reconfigure ASconfig
@06:38:44.65 AS.seek 0.5ml
@06:39:13.68 delay 1 second
@06:39:14.70 AFV.select :holdingCoil
@06:39:14.81 ATV.dial :extSrc
@06:39:14.83 AS.reconfigure ASslow1
@06:39:14.89 AS.seek 0ml
@06:40:55.82 AFV.select :carrierSolution
@06:40:55.94 delay 2 seconds
@06:40:57.96 AS.reconfigure ASconfig
@06:40:58.02 AS.seek 0.5ml
@06:41:27.04 delay 1 second
@06:41:28.06 AFV.select :holdingCoil
@06:41:28.17 ATV.dial :extSrc
@06:41:28.19 AS.reconfigure ASslow1
@06:41:28.25 AS.seek 0ml
@06:43:09.07 ATV.dial :air2
@06:43:10.48 AS.reconfigure ASslow0
@06:43:10.54 AS.seek 0.5ml
@06:44:01.17 ATV.dial :extSrc
@06:44:02.76 AS.seek 0.02ml
@06:44:51.34 delay 1 second
@06:44:52.37 ATV.dial :waste
@06:44:53.75 AS.reconfigure ASconfig
@06:44:53.80 AS.seek 0ml
@06:44:55.24 AFV.select :holdingCoil
@06:44:55.26 ATV.dial :waste
@06:44:55.28 AS.seek 0ml
@06:44:55.36 AFV.select :carrierSolution
@06:44:55.48 delay 2 seconds
@06:44:57.50 AS.seek 0.5ml
@06:45:26.48 delay 1 second
@06:45:27.50 AFV.select :holdingCoil
@06:45:27.61 ATV.dial :air3
@06:45:28.51 AS.reconfigure ASslow1
@06:45:28.57 AS.seek 0.52ml
@06:45:32.85 ATV.dial :air3
@06:45:32.94 AS.seek 0.53ml
@06:45:35.23 delay 2 seconds
@06:45:37.26 ATV.dial :bleach
@06:45:37.74 AS.seek 0.71ml
@06:46:14.20 delay 3 seconds
@06:46:17.23 ATV.dial :air3
@06:46:17.81 delay 3 seconds
@06:46:20.88 AS.seek 0.735ml
@06:46:26.15 delay 3 seconds
@06:46:29.18 ATV.dial :bleach
@06:46:29.82 AS.seek 0.72ml
@06:46:33.23 delay 3 seconds
@06:46:36.26 ATV.dial ASV
@06:46:37.37 ASV.dial :SPErecovery
@06:46:37.46 AS.seek 0.365ml
@06:47:49.12 ATV.dial :waste
@06:47:51.18 AS.reconfigure ASnorm
@06:47:51.23 AS.seek 0ml
@06:48:09.96 ATV.dial :air3
@06:48:10.85 AS.reconfigure ASslow1
@06:48:10.91 AS.seek 0.01ml
@06:48:13.20 delay 2 seconds
@06:48:15.23 ATV.dial :bleach
@06:48:15.71 AS.seek 0.58ml
@06:50:11.04 delay 3 seconds
@06:50:14.07 ATV.dial :air3
@06:50:14.67 delay 3 seconds
@06:50:17.75 AS.seek 0.605ml
@06:50:23.02 delay 3 seconds
@06:50:26.05 ATV.dial :bleach
@06:50:26.71 AS.seek 0.59ml
@06:50:30.01 delay 3 seconds
@06:50:33.04 ATV.dial :ASV
@06:50:34.15 ASV.dial :wasteR
@06:50:34.57 AS.seek 0ml
@06:52:33.48 delay 1:00
@06:53:33.52 ATV.dial ASV
@06:53:33.53 AFV.select :carrierSolution
@06:53:33.65 delay 2 seconds
@06:53:35.67 AS.reconfigure ASconfig
@06:53:35.72 AS.seek 1ml
@06:54:33.48 delay 1 second
@06:54:34.50 AFV.select :holdingCoil
@06:54:34.61 delay 1 second
@06:54:35.63 ASV.dial :wasteR
@06:54:35.64 AS.reconfigure ASslow1
@06:54:35.71 AS.seek 0ml
@06:57:57.10 delay 10 seconds
@06:58:07.14 ATV.dial :air3
@06:58:08.76 AS.seek 0.01ml
@06:58:11.20 delay 2 seconds
@06:58:13.23 ATV.dial :bleach
@06:58:13.87 AS.seek 0.58ml
@07:00:08.81 delay 3 seconds
@07:00:11.84 ATV.dial :air3
@07:00:12.43 delay 3 seconds
@07:00:15.50 AS.seek 0.605ml
@07:00:20.86 delay 3 seconds
@07:00:23.89 ATV.dial :bleach
@07:00:24.54 AS.seek 0.59ml
@07:00:27.87 delay 3 seconds
@07:00:30.90 ATV.dial ASV
@07:00:32.01 ASV.dial :SPEdelivery
@07:00:32.41 AS.seek 0ml
@07:02:31.37 delay 1:00
@07:03:31.40 AFV.select :carrierSolution
@07:03:31.51 delay 2 seconds
@07:03:33.53 AS.reconfigure ASconfig
@07:03:33.59 AS.seek 1ml
@07:04:31.35 delay 1 second
@07:04:32.37 AFV.select :holdingCoil
@07:04:32.48 delay 1 second
@07:04:33.50 ATV.dial ASV
@07:04:33.51 ASV.dial :SPEdelivery
@07:04:33.54 AS.reconfigure ASslow1
@07:04:33.60 AS.seek 0ml
@07:07:55.07 delay 10 seconds
@07:08:05.11 ATV.dial :air2
@07:08:05.99 AS.reconfigure ASslow0
@07:08:06.05 AS.seek 0.8ml
@07:09:26.84 delay 3 seconds
@07:09:29.87 ATV.dial :ASV
@07:09:30.92 ASV.dial :SPEdelivery
@07:09:30.94 AS.reconfigure ASslow1
@07:09:31.01 AS.seek 0.03ml
@07:12:06.21 delay 20 seconds
@07:12:26.25 ATV.dial :waste,via: :air3
@07:12:28.13 AS.reconfigure ASconfig
@07:12:28.19 AS.seek 0ml
@07:12:30.21 SPE.coast
@07:12:30.30 Removing Bleach from Mixing Coil
AFV.select :carrierSolution
@07:12:30.43 delay 2 seconds
@07:12:32.45 AS.seek 0.5ml
@07:13:01.46 delay 1 second
@07:13:02.48 AFV.select :holdingCoil
@07:13:02.59 ATV.dial :ASV
@07:13:04.40 ASV.dial :SPErecovery
@07:13:05.05 AS.reconfigure ASslow1
@07:13:05.11 AS.seek 0.73ml
@07:13:51.65 ASV.dial :wasteD
@07:13:52.27 AS.reconfigure ASnorm
@07:13:52.32 AS.seek 0ml
@07:14:29.43 AFV.select :carrierSolution
@07:14:29.55 delay 2 seconds
@07:14:31.57 AS.reconfigure ASconfig
@07:14:31.63 AS.seek 0.5ml
@07:15:00.67 delay 1 second
@07:15:01.69 AFV.select :holdingCoil
@07:15:01.80 ATV.dial ASV
@07:15:01.81 ASV.dial :SPErecovery
@07:15:02.49 AS.reconfigure ASslow0
@07:15:02.55 AS.seek 0.3ml
@07:15:23.03 ATV.dial :air2
@07:15:24.13 AS.reconfigure ASslow1
@07:15:24.19 AS.seek 0.32ml
@07:15:28.52 ATV.dial ASV
@07:15:29.62 AS.reconfigure ASslow0
@07:15:29.68 AS.seek 0.57ml
@07:15:55.09 ASV.dial :wasteD
@07:15:55.72 AS.reconfigure ASnorm
@07:15:55.78 AS.seek 0ml
@07:16:24.84 AFV.select :carrierSolution
@07:16:24.96 delay 2 seconds
@07:16:26.98 AS.reconfigure ASconfig
@07:16:27.03 AS.seek 0.5ml
@07:16:56.06 delay 1 second
@07:16:57.08 AFV.select :holdingCoil
@07:16:57.19 ATV.dial ASV
@07:16:57.20 ASV.dial :SPErecovery
@07:16:57.88 AS.reconfigure ASslow0
@07:16:57.94 AS.seek 0.3ml
@07:17:18.34 ATV.dial :air2
@07:17:19.43 AS.reconfigure ASslow1
@07:17:19.93 AS.seek 0.32ml
@07:17:24.23 ATV.dial ASV
@07:17:25.34 AS.reconfigure ASslow0
@07:17:25.40 AS.seek 0.57ml
@07:17:50.89 ASV.dial :wasteD
@07:17:51.52 AS.reconfigure ASnorm
@07:17:51.58 AS.seek 0ml
@07:18:20.64 ATV.dial :air2
@07:18:21.69 AS.reconfigure ASslow0
@07:18:21.75 AS.seek 0.3ml
@07:18:52.26 ATV.dial :ASV
@07:18:53.31 ASV.dial :wasteD
@07:18:53.33 AS.reconfigure ASconfig
@07:18:53.40 AS.seek 0.05ml
@07:19:08.07 ATV.dial :waste
@07:19:10.07 AS.seek 0ml
@07:19:13.24 ASV.dial :SPEdelivery
@07:19:14.34 Cleaning PCR
@07:19:14.35 AFV.select :carrierSolution
@07:19:14.47 delay 2 seconds
@07:19:16.49 AS.seek 0.5ml
@07:19:45.54 delay 1 second
@07:19:46.56 AFV.select :holdingCoil
@07:19:46.67 ASV.dial :waste1
@07:19:47.04 ATV.dial :air3
@07:19:47.77 AS.reconfigure ASslow1
@07:19:47.83 AS.seek 0.51ml
@07:19:50.20 delay 2 seconds
@07:19:52.23 ATV.dial :bleach
@07:19:52.70 AS.seek 0.81ml
@07:20:53.32 delay 3 seconds
@07:20:56.35 ATV.dial :air3
@07:20:56.93 delay 3 seconds
@07:21:00.00 AS.seek 0.835ml
@07:21:05.31 delay 3 seconds
@07:21:08.34 ATV.dial :bleach
@07:21:08.98 AS.seek 0.82ml
@07:21:12.23 delay 3 seconds
@07:21:15.26 ARV.dial :waste
@07:21:15.89 delay 3 seconds
@07:21:18.92 ATV.dial :ARV
@07:21:19.84 AS.reconfigure ASconfig
@07:21:19.90 AS.seek 0.47ml
@07:21:40.29 AFV.select :carrierSolution
@07:21:40.41 delay 2 seconds
@07:21:42.43 AS.seek 1ml
@07:22:13.17 delay 1 second
@07:22:14.19 AFV.select :holdingCoil
@07:22:14.30 delay 1 second
@07:22:15.32 AS.reconfigure ASfast
@07:22:15.38 AS.seek 0ml
@07:22:42.70 AFV.select :carrierSolution
@07:22:42.82 delay 2 seconds
@07:22:44.84 AS.reconfigure ASconfig
@07:22:44.89 AS.seek 1ml
@07:23:42.65 delay 1 second
@07:23:43.67 AFV.select :holdingCoil
@07:23:43.78 delay 1 second
@07:23:44.80 AS.reconfigure ASfast
@07:23:44.86 AS.seek 0ml
@07:24:12.21 AFV.select :carrierSolution
@07:24:12.33 delay 2 seconds
@07:24:14.35 AS.reconfigure ASconfig
@07:24:14.40 AS.seek 1ml
@07:25:12.14 delay 1 second
@07:25:13.16 AFV.select :holdingCoil
@07:25:13.27 delay 1 second
@07:25:14.29 AS.reconfigure ASfast
@07:25:14.35 AS.seek 0ml
@07:25:41.68 ARV.dial between carrier and air1
@07:25:42.20 ATV.dial :air3
@07:25:43.55 AS.reconfigure ASslow1
@07:25:43.61 AS.seek 0.01ml
@07:25:46.04 delay 2 seconds
@07:25:48.07 ATV.dial :bleach
@07:25:48.71 AS.seek 0.81ml
@07:28:29.96 delay 3 seconds
@07:28:32.99 ATV.dial :air3
@07:28:33.57 delay 3 seconds
@07:28:36.64 AS.seek 0.835ml
@07:28:41.96 delay 3 seconds
@07:28:44.99 ATV.dial :bleach
@07:28:45.63 AS.seek 0.82ml
@07:28:48.92 delay 3 seconds
@07:28:51.95 ATV.dial PCR
@07:28:52.36 AS.reconfigure ASconfig
@07:28:52.41 AS.seek 0ml
@07:29:39.82 PCR.program :bleachProfile
@07:29:39.90 PCR program should complete in < 2:45
@07:29:39.91 PCR.run 10:00
@07:32:42.19 PCR.off
@07:32:42.23 AFV.select :carrierSolution
@07:32:42.34 delay 2 seconds
@07:32:44.36 AS.seek 1ml
@07:33:42.12 delay 1 second
@07:33:43.14 AFV.select :holdingCoil
@07:33:43.25 delay 1 second
@07:33:44.27 AS.reconfigure ASfast
@07:33:44.33 AS.seek 0ml
@07:34:11.65 delay 3 seconds
@07:34:14.68 AFV.select :carrierSolution
@07:34:14.79 delay 2 seconds
@07:34:16.81 AS.reconfigure ASconfig
@07:34:16.87 AS.seek 1ml
@07:35:14.64 delay 1 second
@07:35:15.66 AFV.select :holdingCoil
@07:35:15.77 delay 1 second
@07:35:16.79 AS.reconfigure ASfast
@07:35:16.84 AS.seek 0ml
@07:35:44.20 delay 3 seconds
@07:35:47.22 AFV.select :carrierSolution
@07:35:47.33 delay 2 seconds
@07:35:49.35 AS.reconfigure ASconfig
@07:35:49.41 AS.seek 1ml
@07:36:47.17 delay 1 second
@07:36:48.19 AFV.select :holdingCoil
@07:36:48.30 delay 1 second
@07:36:49.32 AS.reconfigure ASfast
@07:36:49.38 AS.seek 0ml
@07:37:16.71 delay 3 seconds
@07:37:19.73 AFV.select :carrierSolution
@07:37:19.84 delay 2 seconds
@07:37:21.86 AS.reconfigure ASconfig
@07:37:21.92 AS.seek 1ml
@07:38:19.67 delay 1 second
@07:38:20.69 AFV.select :holdingCoil
@07:38:20.80 delay 1 second
@07:38:21.82 AS.reconfigure ASfast
@07:38:21.88 AS.seek 0ml
@07:38:49.20 delay 3 seconds
@07:38:52.22 AFV.select :carrierSolution
@07:38:52.33 delay 2 seconds
@07:38:54.35 AS.reconfigure ASconfig
@07:38:54.41 AS.seek 1ml
@07:39:52.17 delay 1 second
@07:39:53.19 AFV.select :holdingCoil
@07:39:53.31 delay 1 second
@07:39:54.33 AS.reconfigure ASnorm
@07:39:54.38 AS.seek 0ml
@07:40:45.06 AFV.select :carrierSolution
@07:40:45.17 delay 2 seconds
@07:40:47.19 AS.reconfigure ASconfig
@07:40:47.24 AS.seek 1ml
@07:41:45.04 delay 1 second
@07:41:46.06 AFV.select :holdingCoil
@07:41:46.17 delay 1 second
@07:41:47.19 ATV.dial :waste
@07:41:48.47 AS.reconfigure ASnorm
@07:41:48.53 AS.seek 0.5ml
@07:42:14.07 ATV.dial :waste2
@07:42:15.43 AS.seek 0ml
@07:42:41.04 <MAIN> Cleaning PCR
AFV.select :carrierSolution
@07:42:41.17 delay 2 seconds
@07:42:43.19 AS.reconfigure ASconfig
@07:42:43.24 AS.seek 0.5ml
@07:43:12.28 delay 1 second
@07:43:13.30 AFV.select :holdingCoil
@07:43:13.41 ASV.dial :waste1
@07:43:13.42 ATV.dial :air3
@07:43:15.48 AS.reconfigure ASslow1
@07:43:15.54 AS.seek 0.51ml
@07:43:17.93 delay 2 seconds
@07:43:19.96 ATV.dial :bleach
@07:43:20.45 AS.seek 0.81ml
@07:44:21.04 delay 3 seconds
@07:44:24.07 ATV.dial :air3
@07:44:24.67 delay 3 seconds
@07:44:27.74 AS.seek 0.835ml
@07:44:33.14 delay 3 seconds
@07:44:36.17 ATV.dial :bleach
@07:44:36.80 AS.seek 0.82ml
@07:44:40.09 delay 3 seconds
@07:44:43.12 ARV.dial :waste
@07:44:43.64 delay 3 seconds
@07:44:46.67 ATV.dial :ARV
@07:44:47.59 AS.reconfigure ASconfig
@07:44:48.01 AS.seek 0.47ml
@07:45:08.40 AFV.select :carrierSolution
@07:45:08.52 delay 2 seconds
@07:45:10.54 AS.seek 1ml
@07:45:41.34 delay 1 second
@07:45:42.36 AFV.select :holdingCoil
@07:45:42.47 delay 1 second
@07:45:43.49 AS.reconfigure ASfast
@07:45:43.55 AS.seek 0ml
@07:46:10.90 AFV.select :carrierSolution
@07:46:11.02 delay 2 seconds
@07:46:13.04 AS.reconfigure ASconfig
@07:46:13.09 AS.seek 1ml
@07:47:10.85 delay 1 second
@07:47:11.87 AFV.select :holdingCoil
@07:47:11.98 delay 1 second
@07:47:13.00 AS.reconfigure ASfast
@07:47:13.06 AS.seek 0ml
@07:47:40.37 AFV.select :carrierSolution
@07:47:40.49 delay 2 seconds
@07:47:42.51 AS.reconfigure ASconfig
@07:47:42.57 AS.seek 1ml
@07:48:40.31 delay 1 second
@07:48:41.33 AFV.select :holdingCoil
@07:48:41.44 delay 1 second
@07:48:42.46 AS.reconfigure ASfast
@07:48:42.52 AS.seek 0ml
@07:49:09.87 ARV.dial between carrier and air1
@07:49:10.41 ATV.dial :air3
@07:49:11.74 AS.reconfigure ASslow1
@07:49:11.79 AS.seek 0.01ml
@07:49:14.18 delay 2 seconds
@07:49:16.21 ATV.dial :bleach
@07:49:16.85 AS.seek 0.81ml
@07:51:58.12 delay 3 seconds
@07:52:01.15 ATV.dial :air3
@07:52:01.73 delay 3 seconds
@07:52:04.80 AS.seek 0.835ml
@07:52:10.10 delay 3 seconds
@07:52:13.13 ATV.dial :bleach
@07:52:13.77 AS.seek 0.82ml
@07:52:17.21 delay 3 seconds
@07:52:20.24 ATV.dial PCR
@07:52:20.66 AS.reconfigure ASconfig
@07:52:20.71 AS.seek 0ml
@07:53:08.14 PCR.program :bleachProfile
@07:53:08.21 PCR program should complete in < 2:45
@07:53:08.22 PCR.run 10:00
@07:56:04.05 PCR.off
@07:56:04.09 AFV.select :carrierSolution
@07:56:04.20 delay 2 seconds
@07:56:06.22 AS.seek 1ml
@07:57:03.99 delay 1 second
@07:57:05.01 AFV.select :holdingCoil
@07:57:05.12 delay 1 second
@07:57:06.14 AS.reconfigure ASfast
@07:57:06.19 AS.seek 0ml
@07:57:33.51 delay 3 seconds
@07:57:36.54 AFV.select :carrierSolution
@07:57:36.64 delay 2 seconds
@07:57:38.66 AS.reconfigure ASconfig
@07:57:38.73 AS.seek 1ml
@07:58:36.48 delay 1 second
@07:58:37.50 AFV.select :holdingCoil
@07:58:37.61 delay 1 second
@07:58:38.63 AS.reconfigure ASfast
@07:58:38.68 AS.seek 0ml
@07:59:06.01 delay 3 seconds
@07:59:09.03 AFV.select :carrierSolution
@07:59:09.50 delay 2 seconds
@07:59:11.52 AS.reconfigure ASconfig
@07:59:11.58 AS.seek 1ml
@08:00:09.35 delay 1 second
@08:00:10.37 AFV.select :holdingCoil
@08:00:10.48 delay 1 second
@08:00:11.50 AS.reconfigure ASfast
@08:00:11.56 AS.seek 0ml
@08:00:38.89 delay 3 seconds
@08:00:41.92 AFV.select :carrierSolution
@08:00:42.02 delay 2 seconds
@08:00:44.04 AS.reconfigure ASconfig
@08:00:44.09 AS.seek 1ml
@08:01:41.84 delay 1 second
@08:01:42.86 AFV.select :holdingCoil
@08:01:42.97 delay 1 second
@08:01:43.99 AS.reconfigure ASfast
@08:01:44.04 AS.seek 0ml
@08:02:11.39 delay 3 seconds
@08:02:14.42 AFV.select :carrierSolution
@08:02:14.52 delay 2 seconds
@08:02:16.54 AS.reconfigure ASconfig
@08:02:16.60 AS.seek 1ml
@08:03:14.39 delay 1 second
@08:03:15.41 AFV.select :holdingCoil
@08:03:15.52 delay 1 second
@08:03:16.54 AS.reconfigure ASnorm
@08:03:16.60 AS.seek 0ml
@08:04:07.30 delay 5 seconds
@08:04:12.38 Cleaning PCR
@08:04:12.39 AFV.select :carrierSolution
@08:04:12.51 delay 2 seconds
@08:04:14.53 AS.reconfigure ASconfig
@08:04:14.59 AS.seek 0.5ml
@08:04:43.65 delay 1 second
@08:04:44.67 AFV.select :holdingCoil
@08:04:44.78 ASV.dial :waste1
@08:04:44.79 ATV.dial :air3
@08:04:45.68 AS.reconfigure ASslow1
@08:04:45.73 AS.seek 0.51ml
@08:04:47.99 delay 2 seconds
@08:04:50.02 ATV.dial :bleach
@08:04:50.66 AS.seek 0.81ml
@08:05:51.24 delay 3 seconds
@08:05:54.27 ATV.dial :air3
@08:05:54.85 delay 3 seconds
@08:05:57.92 AS.seek 0.835ml
@08:06:03.21 delay 3 seconds
@08:06:06.24 ATV.dial :bleach
@08:06:06.88 AS.seek 0.82ml
@08:06:10.20 delay 3 seconds
@08:06:13.23 ARV.dial :waste
@08:06:13.73 delay 3 seconds
@08:06:16.76 ATV.dial :ARV
@08:06:17.68 AS.reconfigure ASconfig
@08:06:17.73 AS.seek 0.47ml
@08:06:38.15 AFV.select :carrierSolution
@08:06:38.27 delay 2 seconds
@08:06:40.29 AS.seek 1ml
@08:07:11.03 delay 1 second
@08:07:12.05 AFV.select :holdingCoil
@08:07:12.16 delay 1 second
@08:07:13.18 AS.reconfigure ASfast
@08:07:13.24 AS.seek 0ml
@08:07:40.56 AFV.select :carrierSolution
@08:07:40.68 delay 2 seconds
@08:07:42.70 AS.reconfigure ASconfig
@08:07:42.75 AS.seek 1ml
@08:08:40.51 delay 1 second
@08:08:41.53 AFV.select :holdingCoil
@08:08:41.64 delay 1 second
@08:08:42.66 AS.reconfigure ASfast
@08:08:42.72 AS.seek 0ml
@08:09:10.03 AFV.select :carrierSolution
@08:09:10.15 delay 2 seconds
@08:09:12.17 AS.reconfigure ASconfig
@08:09:12.23 AS.seek 1ml
@08:10:10.01 delay 1 second
@08:10:11.03 AFV.select :holdingCoil
@08:10:11.14 delay 1 second
@08:10:12.16 AS.reconfigure ASfast
@08:10:12.21 AS.seek 0ml
@08:10:39.54 ARV.dial between carrier and air1
@08:10:40.07 ATV.dial :air3
@08:10:41.40 AS.reconfigure ASslow1
@08:10:41.45 AS.seek 0.01ml
@08:10:43.76 delay 2 seconds
@08:10:45.79 ATV.dial :bleach
@08:10:46.43 AS.seek 0.81ml
@08:13:27.56 delay 3 seconds
@08:13:30.59 ATV.dial :air3
@08:13:31.17 delay 3 seconds
@08:13:34.24 AS.seek 0.835ml
@08:13:39.52 delay 3 seconds
@08:13:42.55 ATV.dial :bleach
@08:13:43.20 AS.seek 0.82ml
@08:13:46.49 delay 3 seconds
@08:13:49.52 ATV.dial PCR
@08:13:49.94 AS.reconfigure ASconfig
@08:13:49.99 AS.seek 0ml
@08:14:37.39 PCR.program :bleachProfile
@08:14:37.46 PCR program should complete in < 2:45
@08:14:37.47 PCR.run 10:00
@08:17:30.31 PCR.off
@08:17:30.35 AFV.select :carrierSolution
@08:17:30.46 delay 2 seconds
@08:17:32.48 AS.seek 1ml
@08:18:30.29 delay 1 second
@08:18:31.31 AFV.select :holdingCoil
@08:18:31.42 delay 1 second
@08:18:32.44 AS.reconfigure ASfast
@08:18:32.50 AS.seek 0ml
@08:18:59.84 delay 3 seconds
@08:19:02.87 AFV.select :carrierSolution
@08:19:02.97 delay 2 seconds
@08:19:04.99 AS.reconfigure ASconfig
@08:19:05.05 AS.seek 1ml
@08:20:02.82 delay 1 second
@08:20:03.84 AFV.select :holdingCoil
@08:20:03.95 delay 1 second
@08:20:04.97 AS.reconfigure ASfast
@08:20:05.02 AS.seek 0ml
@08:20:32.35 delay 3 seconds
@08:20:35.38 AFV.select :carrierSolution
@08:20:35.48 delay 2 seconds
@08:20:37.50 AS.reconfigure ASconfig
@08:20:37.56 AS.seek 1ml
@08:21:35.34 delay 1 second
@08:21:36.36 AFV.select :holdingCoil
@08:21:36.47 delay 1 second
@08:21:37.49 AS.reconfigure ASfast
@08:21:37.55 AS.seek 0ml
@08:22:04.89 delay 3 seconds
@08:22:07.92 AFV.select :carrierSolution
@08:22:08.02 delay 2 seconds
@08:22:10.04 AS.reconfigure ASconfig
@08:22:10.10 AS.seek 1ml
@08:23:07.84 delay 1 second
@08:23:08.87 AFV.select :holdingCoil
@08:23:08.97 delay 1 second
@08:23:09.99 AS.reconfigure ASfast
@08:23:10.05 AS.seek 0ml
@08:23:37.40 delay 3 seconds
@08:23:40.43 AFV.select :carrierSolution
@08:23:40.53 delay 2 seconds
@08:23:42.55 AS.reconfigure ASconfig
@08:23:42.62 AS.seek 1ml
@08:24:40.40 delay 1 second
@08:24:41.42 AFV.select :holdingCoil
@08:24:41.53 delay 1 second
@08:24:42.55 AS.reconfigure ASnorm
@08:24:42.60 AS.seek 0ml
@08:25:33.33 delay 5 seconds
@08:25:38.41 Cleaning PCR
@08:25:38.42 AFV.select :carrierSolution
@08:25:38.54 delay 2 seconds
@08:25:40.56 AS.reconfigure ASconfig
@08:25:40.61 AS.seek 0.5ml
@08:26:09.65 delay 1 second
@08:26:10.67 AFV.select :holdingCoil
@08:26:10.78 ASV.dial :waste1
@08:26:10.79 ATV.dial :air3
@08:26:11.68 AS.reconfigure ASslow1
@08:26:11.73 AS.seek 0.51ml
@08:26:14.18 delay 2 seconds
@08:26:16.21 ATV.dial :bleach
@08:26:16.85 AS.seek 0.81ml
@08:27:17.43 delay 3 seconds
@08:27:20.46 ATV.dial :air3
@08:27:21.04 delay 3 seconds
@08:27:24.11 AS.seek 0.835ml
@08:27:29.43 delay 3 seconds
@08:27:32.46 ATV.dial :bleach
@08:27:33.10 AS.seek 0.82ml
@08:27:36.40 delay 3 seconds
@08:27:39.43 ARV.dial :waste
@08:27:39.93 delay 3 seconds
@08:27:42.96 ATV.dial :ARV
@08:27:43.99 AS.reconfigure ASconfig
@08:27:44.04 AS.seek 0.47ml
@08:28:04.46 AFV.select :carrierSolution
@08:28:04.58 delay 2 seconds
@08:28:06.60 AS.seek 1ml
@08:28:37.34 delay 1 second
@08:28:38.36 AFV.select :holdingCoil
@08:28:38.47 delay 1 second
@08:28:39.49 AS.reconfigure ASfast
@08:28:39.55 AS.seek 0ml
@08:29:06.88 AFV.select :carrierSolution
@08:29:07.00 delay 2 seconds
@08:29:09.02 AS.reconfigure ASconfig
@08:29:09.07 AS.seek 1ml
@08:30:06.88 delay 1 second
@08:30:07.90 AFV.select :holdingCoil
@08:30:08.01 delay 1 second
@08:30:09.03 AS.reconfigure ASfast
@08:30:09.09 AS.seek 0ml
@08:30:36.43 AFV.select :carrierSolution
@08:30:36.55 delay 2 seconds
@08:30:38.57 AS.reconfigure ASconfig
@08:30:38.62 AS.seek 1ml
@08:31:36.38 delay 1 second
@08:31:37.41 AFV.select :holdingCoil
@08:31:37.51 delay 1 second
@08:31:38.53 AS.reconfigure ASfast
@08:31:38.59 AS.seek 0ml
@08:32:05.92 ARV.dial between carrier and air1
@08:32:06.46 ATV.dial :air3
@08:32:07.80 AS.reconfigure ASslow1
@08:32:08.22 AS.seek 0.01ml
@08:32:10.67 delay 2 seconds
@08:32:12.70 ATV.dial :bleach
@08:32:13.34 AS.seek 0.81ml
@08:34:54.51 delay 3 seconds
@08:34:57.54 ATV.dial :air3
@08:34:58.12 delay 3 seconds
@08:35:01.20 AS.seek 0.835ml
@08:35:06.49 delay 3 seconds
@08:35:09.52 ATV.dial :bleach
@08:35:10.16 AS.seek 0.82ml
@08:35:13.46 delay 3 seconds
@08:35:16.49 ATV.dial PCR
@08:35:16.93 AS.reconfigure ASconfig
@08:35:16.98 AS.seek 0ml
@08:36:04.37 PCR.program :bleachProfile
@08:36:04.44 PCR program should complete in < 2:45
@08:36:04.45 PCR.run 10:00
@08:39:00.30 PCR.off
@08:39:00.34 AFV.select :carrierSolution
@08:39:00.45 delay 2 seconds
@08:39:02.47 AS.seek 1ml
@08:40:00.23 delay 1 second
@08:40:01.25 AFV.select :holdingCoil
@08:40:01.36 delay 1 second
@08:40:02.38 AS.reconfigure ASfast
@08:40:02.44 AS.seek 0ml
@08:40:29.74 delay 3 seconds
@08:40:32.76 AFV.select :carrierSolution
@08:40:32.87 delay 2 seconds
@08:40:34.89 AS.reconfigure ASconfig
@08:40:34.95 AS.seek 1ml
@08:41:32.71 delay 1 second
@08:41:33.73 AFV.select :holdingCoil
@08:41:33.84 delay 1 second
@08:41:34.86 AS.reconfigure ASfast
@08:41:34.92 AS.seek 0ml
@08:42:02.24 delay 3 seconds
@08:42:05.27 AFV.select :carrierSolution
@08:42:05.37 delay 2 seconds
@08:42:07.39 AS.reconfigure ASconfig
@08:42:07.44 AS.seek 1ml
@08:43:05.21 delay 1 second
@08:43:06.23 AFV.select :holdingCoil
@08:43:06.34 delay 1 second
@08:43:07.36 AS.reconfigure ASfast
@08:43:07.42 AS.seek 0ml
@08:43:34.74 delay 3 seconds
@08:43:37.76 AFV.select :carrierSolution
@08:43:37.87 delay 2 seconds
@08:43:39.89 AS.reconfigure ASconfig
@08:43:39.95 AS.seek 1ml
@08:44:37.74 delay 1 second
@08:44:38.76 AFV.select :holdingCoil
@08:44:38.87 delay 1 second
@08:44:39.89 AS.reconfigure ASfast
@08:44:39.95 AS.seek 0ml
@08:45:07.27 delay 3 seconds
@08:45:10.30 AFV.select :carrierSolution
@08:45:10.40 delay 2 seconds
@08:45:12.42 AS.reconfigure ASconfig
@08:45:12.47 AS.seek 1ml
@08:46:10.23 delay 1 second
@08:46:11.25 AFV.select :holdingCoil
@08:46:11.36 delay 1 second
@08:46:12.38 AS.reconfigure ASnorm
@08:46:12.43 AS.seek 0ml
@08:47:03.11 delay 5 seconds
@08:47:08.19 <mwj> Session Ended
@11:41:56.16CEST16-Apr-21 <MAIN> ESP2 v3.30 I2C Gateway v3.06 for core bus
@11:41:59.16 
Configuration for mer 7/20/20 brent@mbari.org
/home/esp/esp2/type/shallow/mer/configure.rb
@11:41:59.25 
Configuration for MFB3 by preston@mbari.org
/home/esp/esp2/type/mfb/MFB3/configure.rb
@11:42:05.72 Polling Can every 10:00
@11:42:07.83 CS.configure :default
@11:42:07.87 CS.hold
@11:42:07.92 CS.configure :default
@11:42:08.00 PS.configure :default
@11:42:08.08 SS.configure :default
@11:42:08.12 SS.hold
@11:42:08.17 SS.configure :default
@11:42:08.25 CC.configure :default
@11:42:08.29 CC.hold
@11:42:08.38 CC.configure :default
@11:42:08.54 PC.configure :default
@11:42:08.62 PC.hold
@11:42:08.71 PC.configure :default
@11:42:08.90 Forearm.configure :loose
@11:42:08.98 Forearm.hold
@11:42:09.06 Forearm.configure :loose
@11:42:09.20 Elbow.configure :loose
@11:42:09.24 Elbow.hold
@11:42:09.29 Elbow.configure :loose
@11:42:09.39 SC.configure :loose
@11:42:09.43 SC.hold
@11:42:09.49 SC.configure :loose
@11:42:09.57 SE.configure :default
@11:42:09.61 SE.hold
@11:42:09.69 SE.configure :default
@11:42:09.75 CH.coast
@11:42:09.85 CH.configure :default
@11:42:09.91 PH.coast
@11:42:10.00 PH.configure :default
@11:42:10.19 SPE.coast
@11:42:10.29 SPE.configure SPEconfig
@11:42:10.43 AS.configure ASconfig
@11:42:10.49 AS.hold
@11:42:10.53 AS.configure ASconfig
@11:42:10.57 PCR.off
@11:42:10.60 PCR.pidtemp= 50,0.1,0,300,25,25
@11:42:10.64 PCR.caltemp= 2.5051,0.0372,0
@11:42:10.67 All dwarf controllers running firmware version 3.75
@11:42:10.89 Cleanly exited at 11:41:35CEST16-Apr-21
@11:42:10.93 Loading /home/esp/esp2/mission/GENOMAP_v3.rb mission
@11:42:11.17 <MFBPCR> Extracting Nucleic Acid from extSrc
@11:42:11.21 SPE.hold 2:46:40,55C
@11:42:11.29 ASV.dial :SPEdelivery
@11:42:11.71 <bkha> Session Begun
@11:42:11.79 <MFBPCR> delay 20 seconds
@11:42:15.09 <bkha> -> showlog 100
@11:42:31.83 <MFBPCR> AFV.select :holdingCoil
@11:42:31.94 ATV.dial :air2
@11:42:33.39 AS.reconfigure ASslow1
@11:42:33.45 AS.seek 0.07ml
@11:42:47.88 delay 3 seconds
@11:42:50.90 ATV.dial :extSrc
@11:42:52.39 AS.seek 0.47ml
@11:44:13.11 delay 5 seconds
@11:44:18.19 Solid Phase Extraction Heater at 40.1C
@11:44:18.20 ATV.dial ASV
@11:44:19.07 ASV.dial :SPEdelivery
@11:44:19.15 AS.seek 0.042ml
@11:45:45.51 delay 10 seconds
@11:45:55.55 Rinsing external lysate line extSrc
@11:45:55.56 AFV.select :carrierSolution
@11:45:55.68 delay 2 seconds
@11:45:57.70 AS.reconfigure ASconfig
@11:45:57.75 AS.seek 1ml
@11:46:53.10 delay 1 second
@11:46:54.12 AFV.select :holdingCoil
@11:46:54.22 delay 1 second
@11:46:55.24 ATV.dial :extSrc
@11:46:56.06 AS.reconfigure ASnorm
@11:46:56.11 AS.seek 0ml
@11:47:46.81 ATV.dial :air2
@11:47:48.42 AS.reconfigure ASslow0
@11:47:48.48 AS.seek 0.05ml
@11:47:53.86 ATV.dial :extSrc
@11:47:55.45 AS.seek 0.01ml
@11:47:59.85 ATV.dial :waste
@11:48:01.60 AS.reconfigure ASconfig
@11:48:01.65 AS.seek 0ml
@11:48:02.52 ethanol wash
@11:48:02.53 ATV.dial :air2
@11:48:03.71 AS.reconfigure ASslow2
@11:48:03.77 AS.seek 0.01ml
@11:48:14.40 delay 3 seconds
@11:48:17.42 Pulling 0.4ml of Ethanol Wash
@11:48:17.43 ATV.dial :ethanol
@11:48:18.31 AS.reconfigure ASslow1
@11:48:18.37 AS.seek 0.41ml
@11:49:39.14 delay 3 seconds
@11:49:42.17 ATV.dial :air1
@11:49:42.60 delay 3 seconds
@11:49:45.67 AS.seek 0.435ml
@11:49:51.06 delay 3 seconds
@11:49:54.08 ATV.dial :ethanol
@11:49:54.71 AS.seek 0.42ml
@11:49:58.12 delay 3 seconds
@11:50:01.15 ATV.dial ASV
@11:50:02.54 AS.seek 0.093ml
@11:51:08.61 delay 3 seconds
@11:51:11.64 ATV.dial :waste2
@11:51:12.45 AS.reconfigure ASconfig
@11:51:12.50 AS.seek 0ml
@11:51:18.14 ATV.dial :air2
@11:51:18.57 AS.reconfigure ASslow1
@11:51:18.62 AS.seek 1ml
@11:54:40.01 delay 5 seconds
@11:54:45.03 ATV.dial ASV
@11:54:46.08 ASV.dial :SPEdelivery
@11:54:46.11 AS.seek 0.02ml
@11:58:03.46 delay 5 seconds
@11:58:08.48 ATV.dial :air2
@11:58:09.57 AS.seek 0.52ml
@11:59:50.45 delay 5 seconds
@11:59:55.48 ATV.dial ASV
@11:59:56.53 ASV.dial :wasteR
@11:59:56.93 AS.seek 0.3ml
@12:00:41.47 delay 20 seconds
@12:01:01.51 AS.seek 0.07ml
@12:01:48.17 delay 20 seconds
@12:02:08.20 ATV.dial :waste2
@12:02:09.01 AS.reconfigure ASconfig
@12:02:09.07 AS.seek 0ml
@12:02:13.41 Elution
@12:02:13.42 ATV.dial :air1
@12:02:14.62 AS.reconfigure ASslow1
@12:02:14.67 AS.seek 0.05ml
@12:02:25.08 delay 3 seconds
@12:02:28.11 ATV.dial :ElutionWater
@12:02:28.81 AS.seek 0.11ml
@12:02:41.26 delay 3 seconds
@12:02:44.29 ATV.dial ASV,via: :air2
@12:02:46.52 ASV.dial :SPEelution
@12:02:47.14 AFV.select :carrierSolution
@12:02:47.30 AS.reconfigure ASnorm
@12:02:47.36 AS.seek 0.76ml
@12:03:20.42 delay 3 seconds
@12:03:23.45 AFV.select :holdingCoil
@12:03:23.59 AS.reconfigure ASslow1
@12:03:23.65 AS.seek 0.475ml
@12:04:21.38 delay 5 seconds
@12:04:26.45 AS.reconfigure ASslow2
@12:04:26.51 AS.seek 0.44ml
@12:05:02.19 delay 1:00
@12:06:02.22 pushing eluate to holding coil
@12:06:02.29 AS.seek 0.28ml
@12:08:43.58 delay 30 seconds
@12:09:13.62 ATV.dial :waste,via: :air3
@12:09:15.48 AS.reconfigure ASconfig
@12:09:15.53 AS.seek 0ml
@12:09:31.93 Parking Bleach in LysateSource line and SPEcolumn
@12:09:31.95 ATV.dial :air3
@12:09:32.82 AS.reconfigure ASslow1
@12:09:32.87 AS.seek 0.03ml
@12:09:39.20 ATV.dial :ElutionWater
@12:09:39.84 AS.seek 0.06ml
@12:09:46.15 ATV.dial :air3
@12:09:46.79 AS.seek 0.07ml
@12:09:49.23 delay 2 seconds
@12:09:51.26 ATV.dial :bleach
@12:09:51.73 AS.seek 0.15ml
@12:10:08.09 delay 3 seconds
@12:10:11.12 ATV.dial :air3
@12:10:11.71 delay 3 seconds
@12:10:14.77 AS.seek 0.175ml
@12:10:20.07 delay 3 seconds
@12:10:23.10 ATV.dial :bleach
@12:10:23.73 AS.seek 0.16ml
@12:10:27.07 delay 3 seconds
@12:10:30.09 ATV.dial :extSrc
@12:10:30.74 AS.reconfigure ASconfig
@12:10:30.79 AS.seek 0.015ml
@12:10:39.42 ATV.dial :waste
@12:10:40.98 AS.seek 0ml
@12:10:42.15 ATV.dial :air3
@12:10:43.04 AS.reconfigure ASslow1
@12:10:43.10 AS.seek 0.01ml
@12:10:45.51 delay 2 seconds
@12:10:47.54 ATV.dial :bleach
@12:10:47.99 AS.seek 0.58ml
@12:12:42.89 delay 3 seconds
@12:12:45.92 ATV.dial :air3
@12:12:46.51 delay 3 seconds
@12:12:49.58 AS.seek 0.605ml
@12:12:54.96 delay 3 seconds
@12:12:57.99 ATV.dial :bleach
@12:12:58.62 AS.seek 0.59ml
@12:13:01.90 delay 3 seconds
@12:13:04.93 ATV.dial :ASV
@12:13:06.03 ASV.dial :wasteR
@12:13:06.66 AS.seek 0ml
@12:15:05.57 delay 1:00
@12:16:05.60 AFV.select :carrierSolution
@12:16:05.70 delay 2 seconds
@12:16:07.72 AS.reconfigure ASconfig
@12:16:07.77 AS.seek 1ml
@12:17:05.55 delay 1 second
@12:17:06.57 AFV.select :holdingCoil
@12:17:06.67 delay 1 second
@12:17:07.69 ATV.dial ASV
@12:17:07.70 ASV.dial :wasteR
@12:17:07.73 AS.reconfigure ASslow1
@12:17:07.79 AS.seek 0ml
@12:20:29.33 delay 20 seconds
@12:20:49.37 ATV.dial :air3
@12:20:50.98 AS.seek 0.59ml
@12:22:50.09 delay 3 seconds
@12:22:53.11 ATV.dial :ASV
@12:22:54.62 ASV.dial :wasteR
@12:22:54.71 AS.seek 0.09ml
@12:24:35.54 delay 30 seconds
@12:25:05.58 ATV.dial :extSrc
@12:25:06.40 delay 3 seconds
@12:25:09.42 ATV.dial :ElutionWater
@12:25:10.63 AS.seek 0.12ml
@12:25:16.97 ATV.dial :air3
@12:25:17.64 AS.seek 0.13ml
@12:25:19.93 delay 2 seconds
@12:25:21.96 ATV.dial :bleach
@12:25:22.43 AS.seek 0.23ml
@12:25:42.81 delay 3 seconds
@12:25:45.84 ATV.dial :air3
@12:25:46.43 delay 3 seconds
@12:25:49.50 AS.seek 0.255ml
@12:25:54.79 delay 3 seconds
@12:25:57.82 ATV.dial :bleach
@12:25:58.89 AS.seek 0.24ml
@12:26:02.22 delay 3 seconds
@12:26:05.25 ATV.dial :ASV
@12:26:06.36 ASV.dial :SPEdelivery
@12:26:06.76 AFV.select :carrierSolution
@12:26:06.88 delay 2 seconds
@12:26:08.90 AS.reconfigure ASconfig
@12:26:08.95 AS.seek 0.5ml
@12:26:24.21 delay 1 second
@12:26:25.23 AFV.select :holdingCoil
@12:26:25.37 AS.reconfigure ASslow1
@12:26:25.43 AS.seek 0.17ml
@12:27:32.13 SPE.hold 2:46:40,95C
@12:27:32.18 delay 3:00
@12:30:32.23 AFV.select :carrierSolution
@12:30:32.34 delay 2 seconds
@12:30:34.36 AS.reconfigure ASconfig
@12:30:34.41 AS.seek 1ml
@12:31:22.43 delay 1 second
@12:31:23.45 AFV.select :holdingCoil
@12:31:23.55 delay 1 second
@12:31:24.57 ASV.dial :wasteD
@12:31:25.68 AS.seek 0ml
@12:32:23.46 Priming Eluate
@12:32:23.47 AFV.select :carrierSolution
@12:32:23.59 delay 2 seconds
@12:32:25.61 AS.seek 0.5ml
@12:32:54.64 delay 1 second
@12:32:55.67 AFV.select :holdingCoil
@12:32:55.77 ATV.dial ASV
@12:32:55.78 ASV.dial :SPErecovery
@12:32:56.42 Swish #1 of 2
@12:32:56.51 AS.reconfigure ASslow0
@12:32:56.56 AS.seek 0.6ml
@12:33:07.04 AS.seek 0.5ml
@12:33:17.37 Swish #2 of 2
@12:33:17.46 AS.seek 0.6ml
@12:33:27.92 AS.seek 0.5ml
@12:33:38.30 delay 3 seconds
@12:33:41.36 AS.reconfigure ASslow1
@12:33:41.42 AS.seek 0.684ml
@12:34:18.73 delay 3 seconds
@12:34:21.75 Eluate primed
@12:34:21.76 ATV.dial :waste2
@12:34:22.44 AS.reconfigure ASnorm
@12:34:22.49 AS.seek 0ml
@12:34:57.34 SPE.coast
@12:34:57.42 ASV.dial :SPErecovery
@12:34:57.44 Setting up PCR for SYBRIPc -- writing /var/log/esp/SYBRIPc21apr1612h.pcr
@12:34:57.47 Building PCR Slug from ASV with MM1 mix, PP6 primer
@12:34:57.49 Priming with carrier
@12:34:57.51 ATV.dial ARV
@12:34:58.68 ARV.dial :carrier
@12:34:59.05 AS.seek 0.5ml
@12:35:24.62 AFV.select :carrierSolution
@12:35:24.75 delay 2 seconds
@12:35:26.77 AS.reconfigure ASconfig
@12:35:26.84 AS.seek 1ml
@12:35:55.85 delay 1 second
@12:35:56.87 AFV.select :holdingCoil
@12:35:56.97 delay 1 second
@12:35:57.99 ATV.dial :waste
@12:35:59.60 AS.reconfigure ASnorm
@12:35:59.66 AS.seek 0ml
@12:36:50.36 AFV.select :carrierSolution
@12:36:50.48 delay 2 seconds
@12:36:52.50 AS.reconfigure ASconfig
@12:36:52.56 AS.seek 0.5ml
@12:37:21.58 delay 1 second
@12:37:22.60 AFV.select :holdingCoil
@12:37:22.70 Building Slug
@12:37:22.71 ATV.dial :ARV
@12:37:24.48 delay 2 seconds
@12:37:26.50 ARV.dial :air1
@12:37:26.94 delay 2 seconds
@12:37:29.01 AS.reconfigure ASslow2
@12:37:29.07 AS.seek 0.506ml
@12:37:35.57 delay 2 seconds
@12:37:37.59 ARV.dial :MM1
@12:37:38.23 delay 2 seconds
@12:37:40.25 Adding MM1
@12:37:40.33 AS.seek 0.524ml
@12:37:59.09 delay 25 seconds
@12:38:24.12 ARV.dial :PP6
@12:38:25.46 Adding PP6
@12:38:25.47 delay 2 seconds
@12:38:27.55 AS.seek 0.53ml
@12:38:33.88 delay 10 seconds
@12:38:43.91 ARV.dial :air1
@12:38:44.79 adding ARV air
@12:38:44.80 delay 2 seconds
@12:38:46.88 AS.seek 0.536ml
@12:38:53.61 delay 5 seconds
@12:38:58.64 ARV.dial :carrier
@12:38:59.07 delay 7 seconds
@12:39:06.15 AS.seek 0.594ml
@12:40:04.65 delay 5 seconds
@12:40:09.67 ATV.dial :ASV
@12:40:10.10 delay 2 seconds
@12:40:12.17 AS.seek 0.6ml
@12:40:19.08 delay 4 seconds
@12:40:23.10 ATV.dial ARV
@12:40:23.68 delay 2 seconds
@12:40:25.70 ARV.dial :carrier
@12:40:25.71 delay 2 seconds
@12:40:27.79 AS.seek 0.65ml
@12:41:18.35 delay 4 seconds
@12:41:22.37 ATV.dial PCR
@12:41:23.01 ASV.dial :waste1
@12:41:23.83 ARV.dial :air1
@12:41:24.24 Positioning Slug by 0.324ml
@12:41:24.33 AS.seek 0.326ml
@12:46:50.68 delay 5 seconds
@12:46:55.70 ATV.dial ARV
@12:46:56.52 ASV.dial :PCRlock
@12:46:56.91 delay 5 seconds
@12:47:01.93 Running SYBRIPc Profile60CIQ1 thermocycling
@12:47:01.94 <PCR> PCR.program Profile60CIQ1
@12:47:02.06 Profile60CIQ1 should complete in < 1:56:15
@12:47:02.07 PCR.run 2:30:18.75
@14:26:36.48 PCR.off
@14:26:37.02 <MFBPCR> PCR for SYBRIPc completed -- closed /var/log/esp/SYBRIPc21apr1612h.pcr
@14:26:37.09 Cleaning PCR
@14:26:37.10 AFV.select :carrierSolution
@14:26:37.22 delay 2 seconds
@14:26:39.24 AS.reconfigure ASconfig
@14:26:39.29 AS.seek 0.5ml
@14:26:49.62 delay 1 second
@14:26:50.65 AFV.select :holdingCoil
@14:26:50.75 ASV.dial :waste1
@14:26:51.16 ATV.dial :air3
@14:26:52.52 AS.reconfigure ASslow1
@14:26:52.58 AS.seek 0.51ml
@14:26:54.85 delay 2 seconds
@14:26:56.88 ATV.dial :bleach
@14:26:57.52 AS.seek 0.81ml
@14:27:58.13 delay 3 seconds
@14:28:01.15 ATV.dial :air3
@14:28:01.74 delay 3 seconds
@14:28:04.81 AS.seek 0.835ml
@14:28:10.08 delay 3 seconds
@14:28:13.11 ATV.dial :bleach
@14:28:13.74 AS.seek 0.82ml
@14:28:17.05 delay 3 seconds
@14:28:20.07 ARV.dial :waste
@14:28:20.71 delay 3 seconds
@14:28:23.73 ATV.dial :ARV
@14:28:24.66 AS.reconfigure ASconfig
@14:28:24.71 AS.seek 0.47ml
@14:28:45.10 AFV.select :carrierSolution
@14:28:45.22 delay 2 seconds
@14:28:47.24 AS.seek 1ml
@14:29:17.96 delay 1 second
@14:29:18.99 AFV.select :holdingCoil
@14:29:19.09 delay 1 second
@14:29:20.45 AS.reconfigure ASfast
@14:29:20.56 AS.seek 0ml
@14:29:47.87 AFV.select :carrierSolution
@14:29:47.99 delay 2 seconds
@14:29:50.01 AS.reconfigure ASconfig
@14:29:50.07 AS.seek 1ml
@14:30:47.82 delay 1 second
@14:30:48.84 AFV.select :holdingCoil
@14:30:48.94 delay 1 second
@14:30:49.96 AS.reconfigure ASfast
@14:30:50.02 AS.seek 0ml
@14:31:17.35 AFV.select :carrierSolution
@14:31:17.47 delay 2 seconds
@14:31:19.49 AS.reconfigure ASconfig
@14:31:19.54 AS.seek 1ml
@14:32:17.28 delay 1 second
@14:32:18.31 AFV.select :holdingCoil
@14:32:18.41 delay 1 second
@14:32:19.43 AS.reconfigure ASfast
@14:32:19.49 AS.seek 0ml
@14:32:46.82 ARV.dial between carrier and air1
@14:32:47.36 ATV.dial :air3
@14:32:48.73 AS.reconfigure ASslow1
@14:32:48.79 AS.seek 0.01ml
@14:32:51.13 delay 2 seconds
@14:32:53.15 ATV.dial :bleach
@14:32:53.79 AS.seek 0.81ml
@14:35:34.94 delay 3 seconds
@14:35:37.96 ATV.dial :air3
@14:35:38.55 delay 3 seconds
@14:35:41.62 AS.seek 0.835ml
@14:35:47.00 delay 3 seconds
@14:35:50.03 ATV.dial :bleach
@14:35:50.66 AS.seek 0.82ml
@14:35:54.06 delay 3 seconds
@14:35:57.09 ATV.dial PCR
@14:35:57.52 AS.reconfigure ASconfig
@14:35:57.57 AS.seek 0ml
@14:36:44.99 PCR.program :bleachProfile
@14:36:45.06 PCR program should complete in < 2:45
@14:36:45.07 PCR.run 10:00
@14:39:40.86 PCR.off
@14:39:40.91 AFV.select :carrierSolution
@14:39:41.02 delay 2 seconds
@14:39:43.04 AS.seek 1ml
@14:40:40.81 delay 1 second
@14:40:41.84 AFV.select :holdingCoil
@14:40:41.94 delay 1 second
@14:40:42.96 AS.reconfigure ASfast
@14:40:43.01 AS.seek 0ml
@14:41:10.36 delay 3 seconds
@14:41:13.38 AFV.select :carrierSolution
@14:41:13.48 delay 2 seconds
@14:41:15.50 AS.reconfigure ASconfig
@14:41:15.57 AS.seek 1ml
@14:42:13.35 delay 1 second
@14:42:14.38 AFV.select :holdingCoil
@14:42:14.48 delay 1 second
@14:42:15.50 AS.reconfigure ASfast
@14:42:15.55 AS.seek 0ml
@14:42:42.88 delay 3 seconds
@14:42:45.91 AFV.select :carrierSolution
@14:42:46.01 delay 2 seconds
@14:42:48.03 AS.reconfigure ASconfig
@14:42:48.09 AS.seek 1ml
@14:43:45.84 delay 1 second
@14:43:46.87 AFV.select :holdingCoil
@14:43:46.97 delay 1 second
@14:43:47.99 AS.reconfigure ASfast
@14:43:48.04 AS.seek 0ml
@14:44:15.38 delay 3 seconds
@14:44:18.41 AFV.select :carrierSolution
@14:44:18.51 delay 2 seconds
@14:44:20.53 AS.reconfigure ASconfig
@14:44:20.59 AS.seek 1ml
@14:45:18.37 delay 1 second
@14:45:19.40 AFV.select :holdingCoil
@14:45:19.50 delay 1 second
@14:45:20.52 AS.reconfigure ASfast
@14:45:20.58 AS.seek 0ml
@14:45:47.93 delay 3 seconds
@14:45:50.96 AFV.select :carrierSolution
@14:45:51.06 delay 2 seconds
@14:45:53.08 AS.reconfigure ASconfig
@14:45:53.13 AS.seek 1ml
@14:46:50.88 delay 1 second
@14:46:51.90 AFV.select :holdingCoil
@14:46:52.00 delay 1 second
@14:46:53.02 AS.reconfigure ASnorm
@14:46:53.07 AS.seek 0ml
@14:47:43.79 Setting up PCR for ACYPP2 -- writing /var/log/esp/ACYPP221apr1614h.pcr
@14:47:43.80 Building PCR Slug from ASV with MM1 mix, PP2 primer
@14:47:43.82 Priming with carrier
@14:47:43.84 ATV.dial ARV
@14:47:44.49 ARV.dial :carrier
@14:47:45.27 AS.seek 0.5ml
@14:48:10.84 AFV.select :carrierSolution
@14:48:10.96 delay 2 seconds
@14:48:12.98 AS.reconfigure ASconfig
@14:48:13.05 AS.seek 1ml
@14:48:42.08 delay 1 second
@14:48:43.10 AFV.select :holdingCoil
@14:48:43.20 delay 1 second
@14:48:44.22 ATV.dial :waste
@14:48:46.00 AS.reconfigure ASnorm
@14:48:46.06 AS.seek 0ml
@14:49:36.78 AFV.select :carrierSolution
@14:49:36.91 delay 2 seconds
@14:49:38.93 AS.reconfigure ASconfig
@14:49:38.99 AS.seek 0.5ml
@14:50:08.04 delay 1 second
@14:50:09.06 AFV.select :holdingCoil
@14:50:09.16 Building Slug
@14:50:09.18 ATV.dial :ARV
@14:50:10.94 delay 2 seconds
@14:50:12.96 ARV.dial :air1
@14:50:13.38 delay 2 seconds
@14:50:15.45 AS.reconfigure ASslow2
@14:50:15.50 AS.seek 0.506ml
@14:50:21.78 delay 2 seconds
@14:50:23.81 ARV.dial :MM1
@14:50:24.44 delay 2 seconds
@14:50:26.46 Adding MM1
@14:50:26.54 AS.seek 0.524ml
@14:50:44.75 delay 25 seconds
@14:51:09.77 ARV.dial :PP2
@14:51:10.21 Adding PP2
@14:51:10.22 delay 2 seconds
@14:51:12.30 AS.seek 0.53ml
@14:51:19.02 delay 10 seconds
@14:51:29.05 ARV.dial :air2
@14:51:29.47 adding ARV air
@14:51:29.48 delay 2 seconds
@14:51:31.56 AS.seek 0.536ml
@14:51:37.79 delay 5 seconds
@14:51:42.82 ARV.dial :carrier
@14:51:44.18 delay 7 seconds
@14:51:51.26 AS.seek 0.594ml
@14:52:50.18 delay 5 seconds
@14:52:55.20 ATV.dial :ASV
@14:52:55.63 delay 2 seconds
@14:52:57.70 AS.seek 0.6ml
@14:53:04.12 delay 4 seconds
@14:53:08.15 ATV.dial ARV
@14:53:08.72 delay 2 seconds
@14:53:10.74 ARV.dial :carrier
@14:53:10.75 delay 2 seconds
@14:53:12.83 AS.seek 0.65ml
@14:54:03.61 delay 4 seconds
@14:54:07.63 ATV.dial PCR
@14:54:08.28 ASV.dial :waste1
@14:54:08.30 ARV.dial :air1
@14:54:08.72 Positioning Slug by 0.324ml
@14:54:08.81 AS.seek 0.326ml
@14:59:35.19 delay 5 seconds
@14:59:40.21 ATV.dial ARV
@14:59:41.03 ASV.dial :PCRlock
@14:59:41.44 delay 5 seconds
@14:59:46.46 Running ACYPP2 Profile60CIQ2 thermocycling
@14:59:46.48 <PCR> PCR.program Profile60CIQ2
@14:59:46.59 Profile60CIQ2 should complete in < 1:47:50
@14:59:46.60 PCR.run 2:19:47.5
@16:28:41.81 PCR.off
@16:28:42.42 <MFBPCR> Running ACYPP2 MeltProfile60 thermocycling
@16:28:42.44 <PCR> PCR.program MeltProfile60
@16:28:42.54 MeltProfile60 should complete in < 11:55
@16:28:42.55 PCR.run 19:53.75
@16:36:26.21 PCR.off
@16:36:27.31 <MFBPCR> PCR for ACYPP2 completed -- closed /var/log/esp/ACYPP221apr1614h.pcr
@16:36:27.38 Cleaning PCR
@16:36:27.39 AFV.select :carrierSolution
@16:36:27.51 delay 2 seconds
@16:36:29.53 AS.reconfigure ASconfig
@16:36:29.59 AS.seek 0.5ml
@16:36:39.91 delay 1 second
@16:36:40.94 AFV.select :holdingCoil
@16:36:41.04 ASV.dial :waste1
@16:36:41.44 ATV.dial :air3
@16:36:42.80 AS.reconfigure ASslow1
@16:36:42.86 AS.seek 0.51ml
@16:36:45.13 delay 2 seconds
@16:36:47.15 ATV.dial :bleach
@16:36:47.79 AS.seek 0.81ml
@16:37:48.42 delay 3 seconds
@16:37:51.44 ATV.dial :air3
@16:37:52.03 delay 3 seconds
@16:37:55.10 AS.seek 0.835ml
@16:38:00.52 delay 3 seconds
@16:38:03.55 ATV.dial :bleach
@16:38:04.19 AS.seek 0.82ml
@16:38:07.49 delay 3 seconds
@16:38:10.52 ARV.dial :waste
@16:38:11.16 delay 3 seconds
@16:38:14.18 ATV.dial :ARV
@16:38:15.11 AS.reconfigure ASconfig
@16:38:15.16 AS.seek 0.47ml
@16:38:35.55 AFV.select :carrierSolution
@16:38:35.67 delay 2 seconds
@16:38:37.69 AS.seek 1ml
@16:39:08.44 delay 1 second
@16:39:09.47 AFV.select :holdingCoil
@16:39:09.57 delay 1 second
@16:39:10.59 AS.reconfigure ASfast
@16:39:10.65 AS.seek 0ml
@16:39:37.98 AFV.select :carrierSolution
@16:39:38.10 delay 2 seconds
@16:39:40.12 AS.reconfigure ASconfig
@16:39:40.17 AS.seek 1ml
@16:40:37.93 delay 1 second
@16:40:38.96 AFV.select :holdingCoil
@16:40:39.06 delay 1 second
@16:40:40.08 AS.reconfigure ASfast
@16:40:40.14 AS.seek 0ml
@16:41:07.48 AFV.select :carrierSolution
@16:41:07.60 delay 2 seconds
@16:41:09.62 AS.reconfigure ASconfig
@16:41:09.67 AS.seek 1ml
@16:42:07.41 delay 1 second
@16:42:08.44 AFV.select :holdingCoil
@16:42:08.54 delay 1 second
@16:42:09.56 AS.reconfigure ASfast
@16:42:09.62 AS.seek 0ml
@16:42:36.93 ARV.dial between carrier and air1
@16:42:37.47 ATV.dial :air3
@16:42:38.83 AS.reconfigure ASslow1
@16:42:38.89 AS.seek 0.01ml
@16:42:41.30 delay 2 seconds
@16:42:43.33 ATV.dial :bleach
@16:42:43.97 AS.seek 0.81ml
@16:45:25.25 delay 3 seconds
@16:45:28.27 ATV.dial :air3
@16:45:28.86 delay 3 seconds
@16:45:31.92 AS.seek 0.835ml
@16:45:37.32 delay 3 seconds
@16:45:40.35 ATV.dial :bleach
@16:45:40.99 AS.seek 0.82ml
@16:45:44.27 delay 3 seconds
@16:45:47.30 ATV.dial PCR
@16:45:47.74 AS.reconfigure ASconfig
@16:45:47.79 AS.seek 0ml
@16:46:35.19 PCR.program :bleachProfile
@16:46:35.26 PCR program should complete in < 2:45
@16:46:35.27 PCR.run 10:00
@16:49:31.49 PCR.off
@16:49:31.53 AFV.select :carrierSolution
@16:49:31.64 delay 2 seconds
@16:49:33.66 AS.seek 1ml
@16:50:31.40 delay 1 second
@16:50:32.43 AFV.select :holdingCoil
@16:50:32.53 delay 1 second
@16:50:33.55 AS.reconfigure ASfast
@16:50:33.61 AS.seek 0ml
@16:51:00.95 delay 3 seconds
@16:51:03.97 AFV.select :carrierSolution
@16:51:04.07 delay 2 seconds
@16:51:06.09 AS.reconfigure ASconfig
@16:51:06.14 AS.seek 1ml
@16:52:03.94 delay 1 second
@16:52:04.97 AFV.select :holdingCoil
@16:52:05.07 delay 1 second
@16:52:06.09 AS.reconfigure ASfast
@16:52:06.16 AS.seek 0ml
@16:52:33.49 delay 3 seconds
@16:52:36.51 AFV.select :carrierSolution
@16:52:36.61 delay 2 seconds
@16:52:38.63 AS.reconfigure ASconfig
@16:52:38.69 AS.seek 1ml
@16:53:36.48 delay 1 second
@16:53:37.51 AFV.select :holdingCoil
@16:53:37.61 delay 1 second
@16:53:38.63 AS.reconfigure ASfast
@16:53:38.69 AS.seek 0ml
@16:54:06.05 delay 3 seconds
@16:54:09.07 AFV.select :carrierSolution
@16:54:09.17 delay 2 seconds
@16:54:11.19 AS.reconfigure ASconfig
@16:54:11.24 AS.seek 1ml
@16:55:09.02 delay 1 second
@16:55:10.05 AFV.select :holdingCoil
@16:55:10.15 delay 1 second
@16:55:11.17 AS.reconfigure ASfast
@16:55:11.23 AS.seek 0ml
@16:55:38.58 delay 3 seconds
@16:55:41.60 AFV.select :carrierSolution
@16:55:41.70 delay 2 seconds
@16:55:43.72 AS.reconfigure ASconfig
@16:55:43.79 AS.seek 1ml
@16:56:41.57 delay 1 second
@16:56:42.60 AFV.select :holdingCoil
@16:56:42.70 delay 1 second
@16:56:43.72 AS.reconfigure ASnorm
@16:56:43.77 AS.seek 0ml
@16:57:34.49 Setting up PCR for AOPP1 -- writing /var/log/esp/AOPP121apr1616h.pcr
@16:57:34.51 Building PCR Slug from ASV with MM1 mix, PP1 primer
@16:57:34.53 Priming with carrier
@16:57:34.55 ATV.dial ARV
@16:57:35.19 ARV.dial :carrier
@16:57:35.54 AS.seek 0.5ml
@16:58:01.09 AFV.select :carrierSolution
@16:58:01.21 delay 2 seconds
@16:58:03.23 AS.reconfigure ASconfig
@16:58:03.30 AS.seek 1ml
@16:58:32.32 delay 1 second
@16:58:33.35 AFV.select :holdingCoil
@16:58:33.45 delay 1 second
@16:58:34.47 ATV.dial :waste
@16:58:36.27 AS.reconfigure ASnorm
@16:58:36.33 AS.seek 0ml
@16:59:27.01 AFV.select :carrierSolution
@16:59:27.14 delay 2 seconds
@16:59:29.16 AS.reconfigure ASconfig
@16:59:29.22 AS.seek 0.5ml
@16:59:58.27 delay 1 second
@16:59:59.30 AFV.select :holdingCoil
@16:59:59.40 Building Slug
@16:59:59.42 ATV.dial :ARV
@17:00:01.19 delay 2 seconds
@17:00:03.21 ARV.dial :air1
@17:00:03.65 delay 2 seconds
@17:00:05.73 AS.reconfigure ASslow2
@17:00:05.79 AS.seek 0.506ml
@17:00:11.99 delay 2 seconds
@17:00:14.02 ARV.dial :MM1
@17:00:14.66 delay 2 seconds
@17:00:16.68 Adding MM1
@17:00:16.75 AS.seek 0.524ml
@17:00:35.19 delay 25 seconds
@17:01:00.22 ARV.dial :PP1
@17:01:00.63 Adding PP1
@17:01:00.64 delay 2 seconds
@17:01:02.72 AS.seek 0.53ml
@17:01:09.23 delay 10 seconds
@17:01:19.27 ARV.dial :air1
@17:01:19.69 adding ARV air
@17:01:19.70 delay 2 seconds
@17:01:21.78 AS.seek 0.536ml
@17:01:28.24 delay 5 seconds
@17:01:33.27 ARV.dial :carrier
@17:01:33.69 delay 7 seconds
@17:01:40.76 AS.seek 0.594ml
@17:02:39.30 delay 5 seconds
@17:02:44.33 ATV.dial :ASV
@17:02:44.77 delay 2 seconds
@17:02:46.84 AS.seek 0.6ml
@17:02:53.51 delay 4 seconds
@17:02:57.54 ATV.dial ARV
@17:02:58.11 delay 2 seconds
@17:03:00.13 ARV.dial :carrier
@17:03:00.55 delay 2 seconds
@17:03:02.64 AS.seek 0.65ml
@17:03:53.13 delay 4 seconds
@17:03:57.15 ATV.dial PCR
@17:03:57.83 ASV.dial :waste1
@17:03:57.84 ARV.dial :air1
@17:03:58.26 Positioning Slug by 0.324ml
@17:03:58.35 AS.seek 0.326ml
@17:09:24.42 delay 5 seconds
@17:09:29.45 ATV.dial ARV
@17:09:30.30 ASV.dial :PCRlock
@17:09:30.70 delay 5 seconds
@17:09:35.72 Running AOPP1 Profile60CIQ1 thermocycling
@17:09:35.74 <PCR> PCR.program Profile60CIQ1
@17:09:35.87 Profile60CIQ1 should complete in < 1:56:15
@17:09:35.88 PCR.run 2:30:18.75
@18:48:52.81 PCR.off
@18:48:53.37 <MFBPCR> Running AOPP1 MeltProfile60 thermocycling
@18:48:53.38 <PCR> PCR.program MeltProfile60
@18:48:53.87 MeltProfile60 should complete in < 11:55
@18:48:53.88 PCR.run 19:53.75
@18:56:44.13 PCR.off
@18:56:45.04 <MFBPCR> PCR for AOPP1 completed -- closed /var/log/esp/AOPP121apr1616h.pcr
@18:56:45.11 Cleaning PCR
@18:56:45.12 AFV.select :carrierSolution
@18:56:45.23 delay 2 seconds
@18:56:47.25 AS.reconfigure ASconfig
@18:56:47.31 AS.seek 0.5ml
@18:56:57.60 delay 1 second
@18:56:58.63 AFV.select :holdingCoil
@18:56:58.73 ASV.dial :waste1
@18:56:59.13 ATV.dial :air3
@18:57:00.52 AS.reconfigure ASslow1
@18:57:00.58 AS.seek 0.51ml
@18:57:02.87 delay 2 seconds
@18:57:04.90 ATV.dial :bleach
@18:57:05.55 AS.seek 0.81ml
@18:58:06.10 delay 3 seconds
@18:58:09.13 ATV.dial :air3
@18:58:09.72 delay 3 seconds
@18:58:12.78 AS.seek 0.835ml
@18:58:18.19 delay 3 seconds
@18:58:21.22 ATV.dial :bleach
@18:58:21.86 AS.seek 0.82ml
@18:58:25.18 delay 3 seconds
@18:58:28.21 ARV.dial :waste
@18:58:28.84 delay 3 seconds
@18:58:31.86 ATV.dial :ARV
@18:58:32.80 AS.reconfigure ASconfig
@18:58:32.85 AS.seek 0.47ml
@18:58:53.27 AFV.select :carrierSolution
@18:58:53.39 delay 2 seconds
@18:58:55.41 AS.seek 1ml
@18:59:26.16 delay 1 second
@18:59:27.19 AFV.select :holdingCoil
@18:59:27.29 delay 1 second
@18:59:28.31 AS.reconfigure ASfast
@18:59:28.37 AS.seek 0ml
@18:59:55.71 AFV.select :carrierSolution
@18:59:55.83 delay 2 seconds
@18:59:57.85 AS.reconfigure ASconfig
@18:59:57.91 AS.seek 1ml
@19:00:55.66 delay 1 second
@19:00:56.69 AFV.select :holdingCoil
@19:00:56.79 delay 1 second
@19:00:57.81 AS.reconfigure ASfast
@19:00:57.87 AS.seek 0ml
@19:01:25.19 AFV.select :carrierSolution
@19:01:25.31 delay 2 seconds
@19:01:27.33 AS.reconfigure ASconfig
@19:01:27.38 AS.seek 1ml
@19:02:25.11 delay 1 second
@19:02:26.14 AFV.select :holdingCoil
@19:02:26.24 delay 1 second
@19:02:27.26 AS.reconfigure ASfast
@19:02:27.31 AS.seek 0ml
@19:02:54.64 ARV.dial between carrier and air1
@19:02:55.17 ATV.dial :air3
@19:02:56.53 AS.reconfigure ASslow1
@19:02:56.59 AS.seek 0.01ml
@19:02:58.90 delay 2 seconds
@19:03:00.92 ATV.dial :bleach
@19:03:01.58 AS.seek 0.81ml
@19:05:42.82 delay 3 seconds
@19:05:45.85 ATV.dial :air3
@19:05:46.44 delay 3 seconds
@19:05:49.50 AS.seek 0.835ml
@19:05:54.87 delay 3 seconds
@19:05:57.90 ATV.dial :bleach
@19:05:58.52 AS.seek 0.82ml
@19:06:01.89 delay 3 seconds
@19:06:04.92 ATV.dial PCR
@19:06:05.36 AS.reconfigure ASconfig
@19:06:05.41 AS.seek 0ml
@19:06:52.85 PCR.program :bleachProfile
@19:06:52.93 PCR program should complete in < 2:45
@19:06:52.94 PCR.run 10:00
@19:09:49.15 PCR.off
@19:09:49.20 AFV.select :carrierSolution
@19:09:49.31 delay 2 seconds
@19:09:51.33 AS.seek 1ml
@19:10:49.08 delay 1 second
@19:10:50.11 AFV.select :holdingCoil
@19:10:50.21 delay 1 second
@19:10:51.23 AS.reconfigure ASfast
@19:10:51.29 AS.seek 0ml
@19:11:18.64 delay 3 seconds
@19:11:21.66 AFV.select :carrierSolution
@19:11:21.76 delay 2 seconds
@19:11:23.78 AS.reconfigure ASconfig
@19:11:23.85 AS.seek 1ml
@19:12:21.63 delay 1 second
@19:12:22.66 AFV.select :holdingCoil
@19:12:22.76 delay 1 second
@19:12:23.78 AS.reconfigure ASfast
@19:12:23.84 AS.seek 0ml
@19:12:51.18 delay 3 seconds
@19:12:54.21 AFV.select :carrierSolution
@19:12:54.31 delay 2 seconds
@19:12:56.33 AS.reconfigure ASconfig
@19:12:56.39 AS.seek 1ml
@19:13:54.16 delay 1 second
@19:13:55.19 AFV.select :holdingCoil
@19:13:55.29 delay 1 second
@19:13:56.31 AS.reconfigure ASfast
@19:13:56.37 AS.seek 0ml
@19:14:23.72 delay 3 seconds
@19:14:26.74 AFV.select :carrierSolution
@19:14:26.84 delay 2 seconds
@19:14:28.86 AS.reconfigure ASconfig
@19:14:28.93 AS.seek 1ml
@19:15:26.66 delay 1 second
@19:15:27.69 AFV.select :holdingCoil
@19:15:27.79 delay 1 second
@19:15:28.81 AS.reconfigure ASfast
@19:15:28.87 AS.seek 0ml
@19:15:56.18 delay 3 seconds
@19:15:59.21 AFV.select :carrierSolution
@19:15:59.31 delay 2 seconds
@19:16:01.33 AS.reconfigure ASconfig
@19:16:01.39 AS.seek 1ml
@19:16:59.13 delay 1 second
@19:17:00.16 AFV.select :holdingCoil
@19:17:00.26 delay 1 second
@19:17:01.28 AS.reconfigure ASnorm
@19:17:01.33 AS.seek 0ml
@19:17:52.07 Setting up PCR for AARPP4 -- writing /var/log/esp/AARPP421apr1619h.pcr
@19:17:52.09 Building PCR Slug from ASV with MM2 mix, PP4 primer
@19:17:52.11 Priming with carrier
@19:17:52.13 ATV.dial ARV
@19:17:52.79 ARV.dial :carrier
@19:17:53.14 AS.seek 0.5ml
@19:18:18.71 AFV.select :carrierSolution
@19:18:18.83 delay 2 seconds
@19:18:20.85 AS.reconfigure ASconfig
@19:18:20.91 AS.seek 1ml
@19:18:49.93 delay 1 second
@19:18:50.96 AFV.select :holdingCoil
@19:18:51.06 delay 1 second
@19:18:52.08 ATV.dial :waste
@19:18:53.86 AS.reconfigure ASnorm
@19:18:53.92 AS.seek 0ml
@19:19:44.63 AFV.select :carrierSolution
@19:19:44.75 delay 2 seconds
@19:19:46.77 AS.reconfigure ASconfig
@19:19:46.83 AS.seek 0.5ml
@19:20:15.85 delay 1 second
@19:20:16.88 AFV.select :holdingCoil
@19:20:16.98 Building Slug
@19:20:16.99 ATV.dial :ARV
@19:20:18.75 delay 2 seconds
@19:20:20.77 ARV.dial :air1
@19:20:21.17 delay 2 seconds
@19:20:23.23 AS.reconfigure ASslow2
@19:20:23.30 AS.seek 0.506ml
@19:20:30.45 delay 2 seconds
@19:20:32.48 ARV.dial :MM2
@19:20:34.08 delay 2 seconds
@19:20:36.10 Adding MM2
@19:20:36.18 AS.seek 0.524ml
@19:20:54.55 delay 25 seconds
@19:21:19.57 ARV.dial :PP4
@19:21:20.00 Adding PP4
@19:21:20.01 delay 2 seconds
@19:21:22.09 AS.seek 0.53ml
@19:21:29.21 delay 10 seconds
@19:21:39.25 ARV.dial :air3
@19:21:39.67 adding ARV air
@19:21:39.68 delay 2 seconds
@19:21:41.76 AS.seek 0.536ml
@19:21:48.07 delay 5 seconds
@19:21:53.10 ARV.dial :carrier
@19:21:54.42 delay 7 seconds
@19:22:01.50 AS.seek 0.594ml
@19:23:00.75 delay 5 seconds
@19:23:05.78 ATV.dial :ASV
@19:23:06.20 delay 2 seconds
@19:23:08.27 AS.seek 0.6ml
@19:23:14.50 delay 4 seconds
@19:23:18.52 ATV.dial ARV
@19:23:19.09 delay 2 seconds
@19:23:21.11 ARV.dial :carrier
@19:23:21.12 delay 2 seconds
@19:23:23.20 AS.seek 0.65ml
@19:24:13.76 delay 4 seconds
@19:24:17.78 ATV.dial PCR
@19:24:18.43 ASV.dial :waste1
@19:24:18.45 ARV.dial :air1
@19:24:18.88 Positioning Slug by 0.324ml
@19:24:18.97 AS.seek 0.326ml
@19:29:45.24 delay 5 seconds
@19:29:50.27 ATV.dial ARV
@19:29:51.08 ASV.dial :PCRlock
@19:29:51.50 delay 5 seconds
@19:29:56.52 Running AARPP4 Profile58CIQ2 thermocycling
@19:29:56.53 <PCR> PCR.program Profile58CIQ2
@19:29:56.66 Profile58CIQ2 should complete in < 1:36:15
@19:29:56.67 PCR.run 2:05:18.75
@20:51:05.18 PCR.off
@20:51:05.73 <MFBPCR> Running AARPP4 MeltProfile58 thermocycling
@20:51:05.75 <PCR> PCR.program MeltProfile58
@20:51:05.85 MeltProfile58 should complete in < 12:33
@20:51:05.86 PCR.run 20:41.25
@20:59:02.70 PCR.off
@20:59:03.64 <MFBPCR> PCR for AARPP4 completed -- closed /var/log/esp/AARPP421apr1619h.pcr
@20:59:03.71 Cleaning PCR
@20:59:03.72 AFV.select :carrierSolution
@20:59:03.84 delay 2 seconds
@20:59:05.86 AS.reconfigure ASconfig
@20:59:05.91 AS.seek 0.5ml
@20:59:16.21 delay 1 second
@20:59:17.24 AFV.select :holdingCoil
@20:59:17.34 ASV.dial :waste1
@20:59:17.74 ATV.dial :air3
@20:59:19.11 AS.reconfigure ASslow1
@20:59:19.17 AS.seek 0.51ml
@20:59:21.48 delay 2 seconds
@20:59:23.51 ATV.dial :bleach
@20:59:24.14 AS.seek 0.81ml
@21:00:24.81 delay 3 seconds
@21:00:27.84 ATV.dial :air3
@21:00:28.43 delay 3 seconds
@21:00:31.49 AS.seek 0.835ml
@21:00:36.79 delay 3 seconds
@21:00:39.82 ATV.dial :bleach
@21:00:40.45 AS.seek 0.82ml
@21:00:43.77 delay 3 seconds
@21:00:46.80 ARV.dial :waste
@21:00:47.45 delay 3 seconds
@21:00:50.47 ATV.dial :ARV
@21:00:51.41 AS.reconfigure ASconfig
@21:00:51.47 AS.seek 0.47ml
@21:01:11.89 AFV.select :carrierSolution
@21:01:12.01 delay 2 seconds
@21:01:14.03 AS.seek 1ml
@21:01:44.77 delay 1 second
@21:01:45.80 AFV.select :holdingCoil
@21:01:45.90 delay 1 second
@21:01:46.92 AS.reconfigure ASfast
@21:01:46.98 AS.seek 0ml
@21:02:14.30 AFV.select :carrierSolution
@21:02:14.77 delay 2 seconds
@21:02:16.85 AS.reconfigure ASconfig
@21:02:16.90 AS.seek 1ml
@21:03:14.64 delay 1 second
@21:03:15.67 AFV.select :holdingCoil
@21:03:15.77 delay 1 second
@21:03:16.79 AS.reconfigure ASfast
@21:03:16.85 AS.seek 0ml
@21:03:44.19 AFV.select :carrierSolution
@21:03:44.31 delay 2 seconds
@21:03:46.33 AS.reconfigure ASconfig
@21:03:46.39 AS.seek 1ml
@21:04:44.18 delay 1 second
@21:04:45.20 AFV.select :holdingCoil
@21:04:45.30 delay 1 second
@21:04:46.32 AS.reconfigure ASfast
@21:04:46.38 AS.seek 0ml
@21:05:13.71 ARV.dial between carrier and air1
@21:05:14.25 ATV.dial :air3
@21:05:15.62 AS.reconfigure ASslow1
@21:05:15.71 AS.seek 0.01ml
@21:05:18.19 delay 2 seconds
@21:05:20.22 ATV.dial :bleach
@21:05:20.86 AS.seek 0.81ml
@21:08:02.03 delay 3 seconds
@21:08:05.06 ATV.dial :air3
@21:08:05.65 delay 3 seconds
@21:08:08.71 AS.seek 0.835ml
@21:08:14.01 delay 3 seconds
@21:08:17.04 ATV.dial :bleach
@21:08:17.67 AS.seek 0.82ml
@21:08:21.10 delay 3 seconds
@21:08:24.13 ATV.dial PCR
@21:08:24.56 AS.reconfigure ASconfig
@21:08:24.61 AS.seek 0ml
@21:09:12.03 PCR.program :bleachProfile
@21:09:12.11 PCR program should complete in < 2:45
@21:09:12.12 PCR.run 10:00
@21:12:07.91 PCR.off
@21:12:07.95 AFV.select :carrierSolution
@21:12:08.06 delay 2 seconds
@21:12:10.08 AS.seek 1ml
@21:13:07.85 delay 1 second
@21:13:08.88 AFV.select :holdingCoil
@21:13:08.98 delay 1 second
@21:13:10.00 AS.reconfigure ASfast
@21:13:10.06 AS.seek 0ml
@21:13:37.38 delay 3 seconds
@21:13:40.41 AFV.select :carrierSolution
@21:13:40.51 delay 2 seconds
@21:13:42.53 AS.reconfigure ASconfig
@21:13:42.59 AS.seek 1ml
@21:14:40.33 delay 1 second
@21:14:41.36 AFV.select :holdingCoil
@21:14:41.46 delay 1 second
@21:14:42.48 AS.reconfigure ASfast
@21:14:42.53 AS.seek 0ml
@21:15:09.86 delay 3 seconds
@21:15:12.89 AFV.select :carrierSolution
@21:15:12.99 delay 2 seconds
@21:15:15.01 AS.reconfigure ASconfig
@21:15:15.08 AS.seek 1ml
@21:16:12.81 delay 1 second
@21:16:13.84 AFV.select :holdingCoil
@21:16:13.94 delay 1 second
@21:16:15.31 AS.reconfigure ASfast
@21:16:15.43 AS.seek 0ml
@21:16:42.76 delay 3 seconds
@21:16:45.78 AFV.select :carrierSolution
@21:16:45.88 delay 2 seconds
@21:16:47.90 AS.reconfigure ASconfig
@21:16:47.96 AS.seek 1ml
@21:17:45.75 delay 1 second
@21:17:46.78 AFV.select :holdingCoil
@21:17:46.88 delay 1 second
@21:17:47.90 AS.reconfigure ASfast
@21:17:47.96 AS.seek 0ml
@21:18:15.31 delay 3 seconds
@21:18:18.33 AFV.select :carrierSolution
@21:18:18.43 delay 2 seconds
@21:18:20.45 AS.reconfigure ASconfig
@21:18:20.52 AS.seek 1ml
@21:19:18.32 delay 1 second
@21:19:19.34 AFV.select :holdingCoil
@21:19:19.44 delay 1 second
@21:19:20.46 AS.reconfigure ASnorm
@21:19:20.51 AS.seek 0ml
@21:20:11.27 Setting up PCR for AALPP3 -- writing /var/log/esp/AALPP321apr1621h.pcr
@21:20:11.29 Building PCR Slug from ASV with MM2 mix, PP3 primer
@21:20:11.31 Priming with carrier
@21:20:11.33 ATV.dial ARV
@21:20:12.00 ARV.dial :carrier
@21:20:12.34 AS.seek 0.5ml
@21:20:37.94 AFV.select :carrierSolution
@21:20:38.07 delay 2 seconds
@21:20:40.09 AS.reconfigure ASconfig
@21:20:40.16 AS.seek 1ml
@21:21:09.18 delay 1 second
@21:21:10.20 AFV.select :holdingCoil
@21:21:10.30 delay 1 second
@21:21:11.32 ATV.dial :waste
@21:21:13.11 AS.reconfigure ASnorm
@21:21:13.17 AS.seek 0ml
@21:22:03.88 AFV.select :carrierSolution
@21:22:04.00 delay 2 seconds
@21:22:06.02 AS.reconfigure ASconfig
@21:22:06.08 AS.seek 0.5ml
@21:22:35.11 delay 1 second
@21:22:36.14 AFV.select :holdingCoil
@21:22:36.24 Building Slug
@21:22:36.25 ATV.dial :ARV
@21:22:38.01 delay 2 seconds
@21:22:40.03 ARV.dial :air1
@21:22:40.44 delay 2 seconds
@21:22:42.50 AS.reconfigure ASslow2
@21:22:42.57 AS.seek 0.506ml
@21:22:48.86 delay 2 seconds
@21:22:50.89 ARV.dial :MM2
@21:22:52.47 delay 2 seconds
@21:22:54.49 Adding MM2
@21:22:54.56 AS.seek 0.524ml
@21:23:12.94 delay 25 seconds
@21:23:37.97 ARV.dial :PP3
@21:23:38.37 Adding PP3
@21:23:38.38 delay 2 seconds
@21:23:40.46 AS.seek 0.53ml
@21:23:47.25 delay 10 seconds
@21:23:57.29 ARV.dial :air2
@21:23:57.72 adding ARV air
@21:23:57.73 delay 2 seconds
@21:23:59.81 AS.seek 0.536ml
@21:24:06.30 delay 5 seconds
@21:24:11.33 ARV.dial :carrier
@21:24:12.68 delay 7 seconds
@21:24:19.76 AS.seek 0.594ml
@21:25:18.50 delay 5 seconds
@21:25:23.53 ATV.dial :ASV
@21:25:23.97 delay 2 seconds
@21:25:26.04 AS.seek 0.6ml
@21:25:32.64 delay 4 seconds
@21:25:36.66 ATV.dial ARV
@21:25:37.23 delay 2 seconds
@21:25:39.25 ARV.dial :carrier
@21:25:39.26 delay 2 seconds
@21:25:41.34 AS.seek 0.65ml
@21:26:32.23 delay 4 seconds
@21:26:36.25 ATV.dial PCR
@21:26:36.91 ASV.dial :waste1
@21:26:36.93 ARV.dial :air1
@21:26:37.35 Positioning Slug by 0.324ml
@21:26:37.43 AS.seek 0.326ml
@21:32:03.88 delay 5 seconds
@21:32:08.91 ATV.dial ARV
@21:32:09.74 ASV.dial :PCRlock
@21:32:10.15 delay 5 seconds
@21:32:15.17 Running AALPP3 Profile58CIQ2 thermocycling
@21:32:15.19 <PCR> PCR.program Profile58CIQ2
@21:32:15.30 Profile58CIQ2 should complete in < 1:36:15
@21:32:15.31 PCR.run 2:05:18.75
@22:53:02.90 PCR.off
@22:53:03.44 <MFBPCR> Running AALPP3 MeltProfile58 thermocycling
@22:53:03.45 <PCR> PCR.program MeltProfile58
@22:53:03.56 MeltProfile58 should complete in < 12:33
@22:53:03.57 PCR.run 20:41.25
@23:00:42.41 PCR.off
@23:00:43.35 <MFBPCR> PCR for AALPP3 completed -- closed /var/log/esp/AALPP321apr1621h.pcr
@23:00:43.42 Cleaning PCR
@23:00:43.43 AFV.select :carrierSolution
@23:00:43.94 delay 2 seconds
@23:00:45.96 AS.reconfigure ASconfig
@23:00:46.02 AS.seek 0.5ml
@23:00:56.31 delay 1 second
@23:00:57.34 AFV.select :holdingCoil
@23:00:57.44 ASV.dial :waste1
@23:00:57.84 ATV.dial :air3
@23:00:59.22 AS.reconfigure ASslow1
@23:00:59.28 AS.seek 0.51ml
@23:01:01.58 delay 2 seconds
@23:01:03.60 ATV.dial :bleach
@23:01:04.27 AS.seek 0.81ml
@23:02:04.89 delay 3 seconds
@23:02:07.91 ATV.dial :air3
@23:02:08.50 delay 3 seconds
@23:02:11.57 AS.seek 0.835ml
@23:02:16.95 delay 3 seconds
@23:02:19.98 ATV.dial :bleach
@23:02:20.62 AS.seek 0.82ml
@23:02:23.91 delay 3 seconds
@23:02:26.94 ARV.dial :waste
@23:02:27.57 delay 3 seconds
@23:02:30.59 ATV.dial :ARV
@23:02:31.53 AS.reconfigure ASconfig
@23:02:31.58 AS.seek 0.47ml
@23:02:51.99 AFV.select :carrierSolution
@23:02:52.11 delay 2 seconds
@23:02:54.13 AS.seek 1ml
@23:03:24.92 delay 1 second
@23:03:25.94 AFV.select :holdingCoil
@23:03:26.04 delay 1 second
@23:03:27.06 AS.reconfigure ASfast
@23:03:27.12 AS.seek 0ml
@23:03:54.45 AFV.select :carrierSolution
@23:03:54.57 delay 2 seconds
@23:03:56.59 AS.reconfigure ASconfig
@23:03:56.64 AS.seek 1ml
@23:04:54.39 delay 1 second
@23:04:55.42 AFV.select :holdingCoil
@23:04:55.52 delay 1 second
@23:04:56.54 AS.reconfigure ASfast
@23:04:56.60 AS.seek 0ml
@23:05:23.94 AFV.select :carrierSolution
@23:05:24.05 delay 2 seconds
@23:05:26.07 AS.reconfigure ASconfig
@23:05:26.13 AS.seek 1ml
@23:06:23.91 delay 1 second
@23:06:24.94 AFV.select :holdingCoil
@23:06:25.04 delay 1 second
@23:06:26.06 AS.reconfigure ASfast
@23:06:26.12 AS.seek 0ml
@23:06:53.45 ARV.dial between carrier and air1
@23:06:53.98 ATV.dial :air3
@23:06:55.34 AS.reconfigure ASslow1
@23:06:55.39 AS.seek 0.01ml
@23:06:57.67 delay 2 seconds
@23:06:59.70 ATV.dial :bleach
@23:07:00.34 AS.seek 0.81ml
@23:09:41.47 delay 3 seconds
@23:09:44.50 ATV.dial :air3
@23:09:45.09 delay 3 seconds
@23:09:48.15 AS.seek 0.835ml
@23:09:53.54 delay 3 seconds
@23:09:56.57 ATV.dial :bleach
@23:09:57.20 AS.seek 0.82ml
@23:10:00.49 delay 3 seconds
@23:10:03.52 ATV.dial PCR
@23:10:03.95 AS.reconfigure ASconfig
@23:10:04.00 AS.seek 0ml
@23:10:51.42 PCR.program :bleachProfile
@23:10:51.49 PCR program should complete in < 2:45
@23:10:51.50 PCR.run 10:00
@23:13:47.72 PCR.off
@23:13:47.77 AFV.select :carrierSolution
@23:13:47.88 delay 2 seconds
@23:13:49.90 AS.seek 1ml
@23:14:47.66 delay 1 second
@23:14:48.69 AFV.select :holdingCoil
@23:14:48.79 delay 1 second
@23:14:49.81 AS.reconfigure ASfast
@23:14:49.87 AS.seek 0ml
@23:15:17.20 delay 3 seconds
@23:15:20.23 AFV.select :carrierSolution
@23:15:20.33 delay 2 seconds
@23:15:22.35 AS.reconfigure ASconfig
@23:15:22.41 AS.seek 1ml
@23:16:20.15 delay 1 second
@23:16:21.17 AFV.select :holdingCoil
@23:16:21.27 delay 1 second
@23:16:22.29 AS.reconfigure ASfast
@23:16:22.35 AS.seek 0ml
@23:16:49.69 delay 3 seconds
@23:16:52.72 AFV.select :carrierSolution
@23:16:52.82 delay 2 seconds
@23:16:54.84 AS.reconfigure ASconfig
@23:16:54.90 AS.seek 1ml
@23:17:52.69 delay 1 second
@23:17:53.72 AFV.select :holdingCoil
@23:17:53.82 delay 1 second
@23:17:54.84 AS.reconfigure ASfast
@23:17:54.90 AS.seek 0ml
@23:18:22.24 delay 3 seconds
@23:18:25.27 AFV.select :carrierSolution
@23:18:25.37 delay 2 seconds
@23:18:27.39 AS.reconfigure ASconfig
@23:18:27.45 AS.seek 1ml
@23:19:25.24 delay 1 second
@23:19:26.27 AFV.select :holdingCoil
@23:19:26.37 delay 1 second
@23:19:27.39 AS.reconfigure ASfast
@23:19:27.45 AS.seek 0ml
@23:19:54.78 delay 3 seconds
@23:19:57.81 AFV.select :carrierSolution
@23:19:57.91 delay 2 seconds
@23:19:59.93 AS.reconfigure ASconfig
@23:20:00.00 AS.seek 1ml
@23:20:57.75 delay 1 second
@23:20:58.78 AFV.select :holdingCoil
@23:20:58.88 delay 1 second
@23:20:59.90 AS.reconfigure ASnorm
@23:20:59.95 AS.seek 0ml
@23:21:50.66 Setting up PCR for ACOPP5 -- writing /var/log/esp/ACOPP521apr1623h.pcr
@23:21:50.68 Building PCR Slug from ASV with MM3 mix, PP5 primer
@23:21:50.70 Priming with carrier
@23:21:50.72 ATV.dial ARV
@23:21:51.37 ARV.dial :carrier
@23:21:51.72 AS.seek 0.5ml
@23:22:17.25 AFV.select :carrierSolution
@23:22:17.37 delay 2 seconds
@23:22:19.39 AS.reconfigure ASconfig
@23:22:19.46 AS.seek 1ml
@23:22:48.49 delay 1 second
@23:22:49.52 AFV.select :holdingCoil
@23:22:49.62 delay 1 second
@23:22:50.64 ATV.dial :waste
@23:22:52.43 AS.reconfigure ASnorm
@23:22:52.49 AS.seek 0ml
@23:23:43.17 AFV.select :carrierSolution
@23:23:43.29 delay 2 seconds
@23:23:45.31 AS.reconfigure ASconfig
@23:23:45.37 AS.seek 0.5ml
@23:24:14.47 delay 1 second
@23:24:15.50 AFV.select :holdingCoil
@23:24:15.60 Building Slug
@23:24:15.61 ATV.dial :ARV
@23:24:17.39 delay 2 seconds
@23:24:19.41 ARV.dial :air1
@23:24:19.82 delay 2 seconds
@23:24:21.89 AS.reconfigure ASslow2
@23:24:21.94 AS.seek 0.506ml
@23:24:28.17 delay 2 seconds
@23:24:30.20 ARV.dial :MM3
@23:24:31.31 delay 2 seconds
@23:24:33.33 Adding MM3
@23:24:33.56 AS.seek 0.524ml
@23:24:51.77 delay 25 seconds
@23:25:16.80 ARV.dial :PP5
@23:25:17.22 Adding PP5
@23:25:17.23 delay 2 seconds
@23:25:19.31 AS.seek 0.53ml
@23:25:25.86 delay 10 seconds
@23:25:35.90 ARV.dial :air3
@23:25:36.32 adding ARV air
@23:25:36.33 delay 2 seconds
@23:25:38.41 AS.seek 0.536ml
@23:25:44.94 delay 5 seconds
@23:25:49.97 ARV.dial :carrier
@23:25:51.32 delay 7 seconds
@23:25:58.39 AS.seek 0.594ml
@23:26:56.83 delay 5 seconds
@23:27:01.86 ATV.dial :ASV
@23:27:02.29 delay 2 seconds
@23:27:04.36 AS.seek 0.6ml
@23:27:10.85 delay 4 seconds
@23:27:14.88 ATV.dial ARV
@23:27:15.45 delay 2 seconds
@23:27:17.47 ARV.dial :carrier
@23:27:17.48 delay 2 seconds
@23:27:19.56 AS.seek 0.65ml
@23:28:10.31 delay 4 seconds
@23:28:14.34 ATV.dial PCR
@23:28:14.99 ASV.dial :waste1
@23:28:15.42 ARV.dial :air1
@23:28:15.87 Positioning Slug by 0.324ml
@23:28:15.96 AS.seek 0.326ml
@23:33:42.26 delay 5 seconds
@23:33:47.28 ATV.dial ARV
@23:33:48.10 ASV.dial :PCRlock
@23:33:48.51 delay 5 seconds
@23:33:53.53 Running ACOPP5 ProfileIPc thermocycling
@23:33:53.54 <PCR> PCR.program ProfileIPc
@23:33:53.66 ProfileIPc should complete in < 1:26:35
@23:33:53.67 PCR.run 1:53:13.75
@00:42:32.76CEST17-Apr-21 PCR.off
@00:42:33.31 <MFBPCR> PCR for ACOPP5 completed -- closed /var/log/esp/ACOPP521apr1623h.pcr
@00:42:33.39 Cleaning PCR
@00:42:33.40 AFV.select :carrierSolution
@00:42:33.52 delay 2 seconds
@00:42:35.54 AS.reconfigure ASconfig
@00:42:35.59 AS.seek 0.5ml
@00:42:45.88 delay 1 second
@00:42:46.91 AFV.select :holdingCoil
@00:42:47.01 ASV.dial :waste1
@00:42:47.42 ATV.dial :air3
@00:42:48.78 AS.reconfigure ASslow1
@00:42:48.84 AS.seek 0.51ml
@00:42:51.22 delay 2 seconds
@00:42:53.25 ATV.dial :bleach
@00:42:53.91 AS.seek 0.81ml
@00:43:54.64 delay 3 seconds
@00:43:57.67 ATV.dial :air3
@00:43:58.26 delay 3 seconds
@00:44:01.32 AS.seek 0.835ml
@00:44:06.62 delay 3 seconds
@00:44:09.65 ATV.dial :bleach
@00:44:10.28 AS.seek 0.82ml
@00:44:13.58 delay 3 seconds
@00:44:16.61 ARV.dial :waste
@00:44:17.26 delay 3 seconds
@00:44:20.28 ATV.dial :ARV
@00:44:21.22 AS.reconfigure ASconfig
@00:44:21.27 AS.seek 0.47ml
@00:44:41.69 AFV.select :carrierSolution
@00:44:41.80 delay 2 seconds
@00:44:43.82 AS.seek 1ml
@00:45:14.56 delay 1 second
@00:45:15.59 AFV.select :holdingCoil
@00:45:15.69 delay 1 second
@00:45:16.71 AS.reconfigure ASfast
@00:45:16.77 AS.seek 0ml
@00:45:44.10 AFV.select :carrierSolution
@00:45:44.22 delay 2 seconds
@00:45:46.24 AS.reconfigure ASconfig
@00:45:46.29 AS.seek 1ml
@00:46:44.05 delay 1 second
@00:46:45.08 AFV.select :holdingCoil
@00:46:45.18 delay 1 second
@00:46:46.20 AS.reconfigure ASfast
@00:46:46.26 AS.seek 0ml
@00:47:13.60 AFV.select :carrierSolution
@00:47:13.72 delay 2 seconds
@00:47:15.74 AS.reconfigure ASconfig
@00:47:15.79 AS.seek 1ml
@00:48:13.52 delay 1 second
@00:48:14.55 AFV.select :holdingCoil
@00:48:14.65 delay 1 second
@00:48:15.67 AS.reconfigure ASfast
@00:48:15.73 AS.seek 0ml
@00:48:43.06 ARV.dial between carrier and air1
@00:48:43.61 ATV.dial :air3
@00:48:44.97 AS.reconfigure ASslow1
@00:48:45.03 AS.seek 0.01ml
@00:48:47.41 delay 2 seconds
@00:48:49.44 ATV.dial :bleach
@00:48:50.08 AS.seek 0.81ml
@00:51:31.33 delay 3 seconds
@00:51:34.36 ATV.dial :air3
@00:51:34.95 delay 3 seconds
@00:51:38.01 AS.seek 0.835ml
@00:51:43.30 delay 3 seconds
@00:51:46.33 ATV.dial :bleach
@00:51:46.97 AS.seek 0.82ml
@00:51:50.38 delay 3 seconds
@00:51:53.41 ATV.dial PCR
@00:51:53.84 AS.reconfigure ASconfig
@00:51:53.89 AS.seek 0ml
@00:52:41.33 PCR.program :bleachProfile
@00:52:41.40 PCR program should complete in < 2:45
@00:52:41.41 PCR.run 10:00
@00:55:37.61 PCR.off
@00:55:37.65 AFV.select :carrierSolution
@00:55:37.76 delay 2 seconds
@00:55:39.78 AS.seek 1ml
@00:56:37.57 delay 1 second
@00:56:38.59 AFV.select :holdingCoil
@00:56:38.69 delay 1 second
@00:56:39.71 AS.reconfigure ASfast
@00:56:39.77 AS.seek 0ml
@00:57:07.09 delay 3 seconds
@00:57:10.11 AFV.select :carrierSolution
@00:57:10.21 delay 2 seconds
@00:57:12.23 AS.reconfigure ASconfig
@00:57:12.30 AS.seek 1ml
@00:58:10.06 delay 1 second
@00:58:11.09 AFV.select :holdingCoil
@00:58:11.19 delay 1 second
@00:58:12.21 AS.reconfigure ASfast
@00:58:12.27 AS.seek 0ml
@00:58:39.61 delay 3 seconds
@00:58:42.63 AFV.select :carrierSolution
@00:58:42.73 delay 2 seconds
@00:58:44.75 AS.reconfigure ASconfig
@00:58:44.81 AS.seek 1ml
@00:59:42.56 delay 1 second
@00:59:43.59 AFV.select :holdingCoil
@00:59:43.69 delay 1 second
@00:59:44.71 AS.reconfigure ASfast
@00:59:44.77 AS.seek 0ml
@01:00:12.11 delay 3 seconds
@01:00:15.13 AFV.select :carrierSolution
@01:00:15.23 delay 2 seconds
@01:00:17.25 AS.reconfigure ASconfig
@01:00:17.31 AS.seek 1ml
@01:01:15.06 delay 1 second
@01:01:16.08 AFV.select :holdingCoil
@01:01:16.18 delay 1 second
@01:01:17.20 AS.reconfigure ASfast
@01:01:17.26 AS.seek 0ml
@01:01:44.60 delay 3 seconds
@01:01:47.63 AFV.select :carrierSolution
@01:01:47.73 delay 2 seconds
@01:01:49.75 AS.reconfigure ASconfig
@01:01:49.81 AS.seek 1ml
@01:02:47.56 delay 1 second
@01:02:48.59 AFV.select :holdingCoil
@01:02:48.69 delay 1 second
@01:02:49.71 AS.reconfigure ASnorm
@01:02:49.76 AS.seek 0ml
@01:03:40.45 Setting up PCR for AIPc -- writing /var/log/esp/AIPc21apr1701h.pcr
@01:03:40.47 Building PCR Slug from ASV with MM3 mix, PP6 primer
@01:03:40.49 Priming with carrier
@01:03:40.51 ATV.dial ARV
@01:03:41.16 ARV.dial :carrier
@01:03:41.52 AS.seek 0.5ml
@01:04:07.05 AFV.select :carrierSolution
@01:04:07.18 delay 2 seconds
@01:04:09.20 AS.reconfigure ASconfig
@01:04:09.26 AS.seek 1ml
@01:04:38.27 delay 1 second
@01:04:39.30 AFV.select :holdingCoil
@01:04:39.40 delay 1 second
@01:04:40.42 ATV.dial :waste
@01:04:42.20 AS.reconfigure ASnorm
@01:04:42.26 AS.seek 0ml
@01:05:32.92 AFV.select :carrierSolution
@01:05:33.04 delay 2 seconds
@01:05:35.06 AS.reconfigure ASconfig
@01:05:35.12 AS.seek 0.5ml
@01:06:04.19 delay 1 second
@01:06:05.22 AFV.select :holdingCoil
@01:06:05.32 Building Slug
@01:06:05.33 ATV.dial :ARV
@01:06:07.09 delay 2 seconds
@01:06:09.11 ARV.dial :air1
@01:06:09.52 delay 2 seconds
@01:06:11.58 AS.reconfigure ASslow2
@01:06:11.65 AS.seek 0.506ml
@01:06:18.10 delay 2 seconds
@01:06:20.13 ARV.dial :MM3
@01:06:21.23 delay 2 seconds
@01:06:23.25 Adding MM3
@01:06:23.32 AS.seek 0.524ml
@01:06:41.83 delay 25 seconds
@01:07:06.86 ARV.dial :PP6
@01:07:07.26 Adding PP6
@01:07:07.27 delay 2 seconds
@01:07:09.35 AS.seek 0.53ml
@01:07:15.60 delay 10 seconds
@01:07:25.64 ARV.dial :air1
@01:07:26.51 adding ARV air
@01:07:26.52 delay 2 seconds
@01:07:28.60 AS.seek 0.536ml
@01:07:34.81 delay 5 seconds
@01:07:39.84 ARV.dial :carrier
@01:07:40.25 delay 7 seconds
@01:07:47.33 AS.seek 0.594ml
@01:08:46.40 delay 5 seconds
@01:08:51.42 ATV.dial :ASV
@01:08:51.87 delay 2 seconds
@01:08:53.94 AS.seek 0.6ml
@01:09:00.89 delay 4 seconds
@01:09:04.92 ATV.dial ARV
@01:09:05.51 delay 2 seconds
@01:09:07.53 ARV.dial :carrier
@01:09:07.54 delay 2 seconds
@01:09:09.62 AS.seek 0.65ml
@01:10:00.34 delay 4 seconds
@01:10:04.36 ATV.dial PCR
@01:10:05.01 ASV.dial :waste1
@01:10:05.02 ARV.dial :air1
@01:10:05.44 Positioning Slug by 0.324ml
@01:10:05.53 AS.seek 0.326ml
@01:15:31.93 delay 5 seconds
@01:15:36.95 ATV.dial ARV
@01:15:37.76 ASV.dial :PCRlock
@01:15:38.17 delay 5 seconds
@01:15:43.19 Running AIPc ProfileIPc thermocycling
@01:15:43.20 <PCR> PCR.program ProfileIPc
@01:15:43.33 ProfileIPc should complete in < 1:26:35
@01:15:43.34 PCR.run 1:53:13.75
@02:24:22.02 PCR.off
@02:24:22.56 <MFBPCR> PCR for AIPc completed -- closed /var/log/esp/AIPc21apr1701h.pcr
@02:24:22.58 Full MFB Decontamination
@02:24:22.60 Decon extraction column and decon extSrc
@02:24:22.63 SPE.hold 2:46:40,55C
@02:24:22.68 ASV.dial :SPEdelivery
@02:24:23.36 delay 20 seconds
@02:24:43.39 AFV.select :carrierSolution
@02:24:43.49 delay 2 seconds
@02:24:45.51 AS.reconfigure ASconfig
@02:24:45.57 AS.seek 0.5ml
@02:24:55.85 delay 1 second
@02:24:56.88 AFV.select :holdingCoil
@02:24:56.98 ATV.dial :air1
@02:24:59.25 AS.reconfigure ASslow1
@02:24:59.31 AS.seek 0.52ml
@02:25:03.60 ATV.dial :ASV
@02:25:05.38 ASV.dial :SPErecovery
@02:25:06.08 AS.seek 0.62ml
@02:25:26.49 ATV.dial :waste
@02:25:28.70 AS.reconfigure ASnorm
@02:25:28.76 AS.seek 0ml
@02:26:00.34 deconEluateRecoveryLine extSrc
@02:26:00.35 ATV.dial :waste
@02:26:00.38 AS.seek 0ml
@02:26:00.46 ATV.dial :air3
@02:26:01.22 AS.reconfigure ASslow1
@02:26:01.28 AS.seek 0.01ml
@02:26:03.60 delay 2 seconds
@02:26:05.63 ATV.dial :bleach
@02:26:06.16 AS.seek 0.31ml
@02:27:06.79 delay 3 seconds
@02:27:09.81 ATV.dial :air3
@02:27:10.45 delay 3 seconds
@02:27:13.52 AS.seek 0.335ml
@02:27:18.73 delay 3 seconds
@02:27:21.76 ATV.dial :bleach
@02:27:22.44 AS.seek 0.32ml
@02:27:25.74 delay 3 seconds
@02:27:28.77 ATV.dial :extSrc
@02:27:29.45 AS.seek 0ml
@02:28:34.00 AFV.select :carrierSolution
@02:28:34.12 delay 2 seconds
@02:28:36.14 AS.reconfigure ASconfig
@02:28:36.20 AS.seek 0.5ml
@02:29:05.24 delay 1 second
@02:29:06.27 AFV.select :holdingCoil
@02:29:06.37 ATV.dial :extSrc
@02:29:06.39 AS.reconfigure ASslow1
@02:29:06.45 AS.seek 0ml
@02:30:47.28 AFV.select :carrierSolution
@02:30:47.40 delay 2 seconds
@02:30:49.42 AS.reconfigure ASconfig
@02:30:49.48 AS.seek 0.5ml
@02:31:18.50 delay 1 second
@02:31:19.53 AFV.select :holdingCoil
@02:31:19.63 ATV.dial :extSrc
@02:31:19.65 AS.reconfigure ASslow1
@02:31:19.72 AS.seek 0ml
@02:33:00.53 AFV.select :carrierSolution
@02:33:00.65 delay 2 seconds
@02:33:02.67 AS.reconfigure ASconfig
@02:33:02.73 AS.seek 0.5ml
@02:33:31.77 delay 1 second
@02:33:32.80 AFV.select :holdingCoil
@02:33:32.90 ATV.dial :extSrc
@02:33:32.92 AS.reconfigure ASslow1
@02:33:32.99 AS.seek 0ml
@02:35:13.81 AFV.select :carrierSolution
@02:35:13.93 delay 2 seconds
@02:35:15.95 AS.reconfigure ASconfig
@02:35:16.01 AS.seek 0.5ml
@02:35:45.06 delay 1 second
@02:35:46.08 AFV.select :holdingCoil
@02:35:46.18 ATV.dial :extSrc
@02:35:46.20 AS.reconfigure ASslow1
@02:35:46.27 AS.seek 0ml
@02:37:27.08 ATV.dial :air2
@02:37:28.50 AS.reconfigure ASslow0
@02:37:28.56 AS.seek 0.5ml
@02:38:19.17 ATV.dial :extSrc
@02:38:20.75 AS.seek 0.02ml
@02:39:09.36 delay 1 second
@02:39:10.39 ATV.dial :waste
@02:39:11.76 AS.reconfigure ASconfig
@02:39:11.82 AS.seek 0ml
@02:39:13.25 AFV.select :holdingCoil
@02:39:13.27 ATV.dial :waste
@02:39:13.30 AS.seek 0ml
@02:39:13.38 AFV.select :carrierSolution
@02:39:13.48 delay 2 seconds
@02:39:15.50 AS.seek 0.5ml
@02:39:44.52 delay 1 second
@02:39:45.55 AFV.select :holdingCoil
@02:39:45.65 ATV.dial :air3
@02:39:46.55 AS.reconfigure ASslow1
@02:39:46.61 AS.seek 0.52ml
@02:39:51.39 ATV.dial :air3
@02:39:51.48 AS.seek 0.53ml
@02:39:53.81 delay 2 seconds
@02:39:55.83 ATV.dial :bleach
@02:39:56.31 AS.seek 0.71ml
@02:40:32.76 delay 3 seconds
@02:40:35.78 ATV.dial :air3
@02:40:36.39 delay 3 seconds
@02:40:39.46 AS.seek 0.735ml
@02:40:44.76 delay 3 seconds
@02:40:47.79 ATV.dial :bleach
@02:40:48.45 AS.seek 0.72ml
@02:40:51.75 delay 3 seconds
@02:40:54.77 ATV.dial ASV
@02:40:55.86 ASV.dial :SPErecovery
@02:40:55.95 AS.seek 0.365ml
@02:42:07.60 ATV.dial :waste
@02:42:09.64 AS.reconfigure ASnorm
@02:42:09.70 AS.seek 0ml
@02:42:28.44 ATV.dial :air3
@02:42:29.33 AS.reconfigure ASslow1
@02:42:29.39 AS.seek 0.01ml
@02:42:31.79 delay 2 seconds
@02:42:33.81 ATV.dial :bleach
@02:42:34.30 AS.seek 0.58ml
@02:44:29.22 delay 3 seconds
@02:44:32.25 ATV.dial :air3
@02:44:32.84 delay 3 seconds
@02:44:35.91 AS.seek 0.605ml
@02:44:41.20 delay 3 seconds
@02:44:44.22 ATV.dial :bleach
@02:44:44.86 AS.seek 0.59ml
@02:44:48.17 delay 3 seconds
@02:44:51.20 ATV.dial :ASV
@02:44:52.32 ASV.dial :wasteR
@02:44:52.73 AS.seek 0ml
@02:46:51.67 delay 1:00
@02:47:51.70 ATV.dial ASV
@02:47:51.71 AFV.select :carrierSolution
@02:47:51.83 delay 2 seconds
@02:47:53.85 AS.reconfigure ASconfig
@02:47:53.90 AS.seek 1ml
@02:48:51.67 delay 1 second
@02:48:52.69 AFV.select :holdingCoil
@02:48:52.79 delay 1 second
@02:48:53.81 ASV.dial :wasteR
@02:48:53.83 AS.reconfigure ASslow1
@02:48:53.90 AS.seek 0ml
@02:52:15.29 delay 10 seconds
@02:52:25.32 ATV.dial :air3
@02:52:26.92 AS.seek 0.01ml
@02:52:29.24 delay 2 seconds
@02:52:31.27 ATV.dial :bleach
@02:52:31.92 AS.seek 0.58ml
@02:54:26.81 delay 3 seconds
@02:54:29.84 ATV.dial :air3
@02:54:30.45 delay 3 seconds
@02:54:33.52 AS.seek 0.605ml
@02:54:38.89 delay 3 seconds
@02:54:41.92 ATV.dial :bleach
@02:54:42.58 AS.seek 0.59ml
@02:54:45.98 delay 3 seconds
@02:54:49.00 ATV.dial ASV
@02:54:50.11 ASV.dial :SPEdelivery
@02:54:50.50 AS.seek 0ml
@02:56:49.43 delay 1:00
@02:57:49.46 AFV.select :carrierSolution
@02:57:49.56 delay 2 seconds
@02:57:51.58 AS.reconfigure ASconfig
@02:57:51.64 AS.seek 1ml
@02:58:49.40 delay 1 second
@02:58:50.42 AFV.select :holdingCoil
@02:58:50.52 delay 1 second
@02:58:51.54 ATV.dial ASV
@02:58:51.55 ASV.dial :SPEdelivery
@02:58:51.57 AS.reconfigure ASslow1
@02:58:51.64 AS.seek 0ml
@03:02:13.17 delay 10 seconds
@03:02:23.21 ATV.dial :air2
@03:02:24.09 AS.reconfigure ASslow0
@03:02:24.15 AS.seek 0.8ml
@03:03:44.95 delay 3 seconds
@03:03:47.97 ATV.dial :ASV
@03:03:49.02 ASV.dial :SPEdelivery
@03:03:49.05 AS.reconfigure ASslow1
@03:03:49.11 AS.seek 0.03ml
@03:06:24.15 delay 20 seconds
@03:06:44.18 ATV.dial :waste,via: :air3
@03:06:46.03 AS.reconfigure ASconfig
@03:06:46.09 AS.seek 0ml
@03:06:48.10 SPE.coast
@03:06:48.19 Removing Bleach from Mixing Coil
@03:06:48.20 AFV.select :carrierSolution
@03:06:48.31 delay 2 seconds
@03:06:50.33 AS.seek 0.5ml
@03:07:19.42 delay 1 second
@03:07:20.45 AFV.select :holdingCoil
@03:07:20.55 ATV.dial :ASV
@03:07:22.50 ASV.dial :SPErecovery
@03:07:23.16 AS.reconfigure ASslow1
@03:07:23.22 AS.seek 0.73ml
@03:08:09.72 ASV.dial :wasteD
@03:08:10.34 AS.reconfigure ASnorm
@03:08:10.39 AS.seek 0ml
@03:08:47.50 AFV.select :carrierSolution
@03:08:47.62 delay 2 seconds
@03:08:49.64 AS.reconfigure ASconfig
@03:08:49.70 AS.seek 0.5ml
@03:09:18.79 delay 1 second
@03:09:19.81 AFV.select :holdingCoil
@03:09:19.91 ATV.dial ASV
@03:09:19.93 ASV.dial :SPErecovery
@03:09:20.61 AS.reconfigure ASslow0
@03:09:20.68 AS.seek 0.3ml
@03:09:41.08 ATV.dial :air2
@03:09:42.03 AS.reconfigure ASslow1
@03:09:42.09 AS.seek 0.32ml
@03:09:46.56 ATV.dial ASV
@03:09:47.67 AS.reconfigure ASslow0
@03:09:47.73 AS.seek 0.57ml
@03:10:13.16 ASV.dial :wasteD
@03:10:13.79 AS.reconfigure ASnorm
@03:10:13.85 AS.seek 0ml
@03:10:42.91 AFV.select :carrierSolution
@03:10:43.03 delay 2 seconds
@03:10:45.05 AS.reconfigure ASconfig
@03:10:45.11 AS.seek 0.5ml
@03:11:14.16 delay 1 second
@03:11:15.19 AFV.select :holdingCoil
@03:11:15.29 ATV.dial ASV
@03:11:15.31 ASV.dial :SPErecovery
@03:11:15.98 AS.reconfigure ASslow0
@03:11:16.04 AS.seek 0.3ml
@03:11:36.50 ATV.dial :air2
@03:11:37.59 AS.reconfigure ASslow1
@03:11:37.65 AS.seek 0.32ml
@03:11:41.98 ATV.dial ASV
@03:11:43.09 AS.reconfigure ASslow0
@03:11:43.15 AS.seek 0.57ml
@03:12:08.63 ASV.dial :wasteD
@03:12:09.26 AS.reconfigure ASnorm
@03:12:09.32 AS.seek 0ml
@03:12:38.36 ATV.dial :air2
@03:12:39.42 AS.reconfigure ASslow0
@03:12:39.47 AS.seek 0.3ml
@03:13:10.05 ATV.dial :ASV
@03:13:11.11 ASV.dial :wasteD
@03:13:11.13 AS.reconfigure ASconfig
@03:13:11.20 AS.seek 0.05ml
@03:13:25.88 ATV.dial :waste
@03:13:27.89 AS.seek 0ml
@03:13:31.01 ASV.dial :SPEdelivery
@03:13:32.11 Cleaning PCR
@03:13:32.12 AFV.select :carrierSolution
@03:13:32.24 delay 2 seconds
@03:13:34.26 AS.seek 0.5ml
@03:14:03.33 delay 1 second
@03:14:04.36 AFV.select :holdingCoil
@03:14:04.46 ASV.dial :waste1
@03:14:04.85 ATV.dial :air3
@03:14:05.58 AS.reconfigure ASslow1
@03:14:05.64 AS.seek 0.51ml
@03:14:07.92 delay 2 seconds
@03:14:09.94 ATV.dial :bleach
@03:14:10.41 AS.seek 0.81ml
@03:15:11.00 delay 3 seconds
@03:15:14.03 ATV.dial :air3
@03:15:14.62 delay 3 seconds
@03:15:17.69 AS.seek 0.835ml
@03:15:22.98 delay 3 seconds
@03:15:26.00 ATV.dial :bleach
@03:15:26.64 AS.seek 0.82ml
@03:15:30.02 delay 3 seconds
@03:15:33.05 ARV.dial :waste
@03:15:33.68 delay 3 seconds
@03:15:36.70 ATV.dial :ARV
@03:15:37.62 AS.reconfigure ASconfig
@03:15:37.67 AS.seek 0.47ml
@03:15:58.11 AFV.select :carrierSolution
@03:15:58.23 delay 2 seconds
@03:16:00.25 AS.seek 1ml
@03:16:31.02 delay 1 second
@03:16:32.05 AFV.select :holdingCoil
@03:16:32.15 delay 1 second
@03:16:33.17 AS.reconfigure ASfast
@03:16:33.23 AS.seek 0ml
@03:17:00.58 AFV.select :carrierSolution
@03:17:00.70 delay 2 seconds
@03:17:02.72 AS.reconfigure ASconfig
@03:17:02.78 AS.seek 1ml
@03:18:00.55 delay 1 second
@03:18:01.58 AFV.select :holdingCoil
@03:18:01.68 delay 1 second
@03:18:02.70 AS.reconfigure ASfast
@03:18:02.76 AS.seek 0ml
@03:18:30.11 AFV.select :carrierSolution
@03:18:30.22 delay 2 seconds
@03:18:32.24 AS.reconfigure ASconfig
@03:18:32.30 AS.seek 1ml
@03:19:30.04 delay 1 second
@03:19:31.06 AFV.select :holdingCoil
@03:19:31.16 delay 1 second
@03:19:32.18 AS.reconfigure ASfast
@03:19:32.24 AS.seek 0ml
@03:19:59.61 ARV.dial between carrier and air1
@03:20:00.15 ATV.dial :air3
@03:20:01.52 AS.reconfigure ASslow1
@03:20:01.58 AS.seek 0.01ml
@03:20:04.03 delay 2 seconds
@03:20:06.06 ATV.dial :bleach
@03:20:06.70 AS.seek 0.81ml
@03:22:47.85 delay 3 seconds
@03:22:50.88 ATV.dial :air3
@03:22:51.48 delay 3 seconds
@03:22:54.55 AS.seek 0.835ml
@03:23:00.35 delay 3 seconds
@03:23:03.38 ATV.dial :bleach
@03:23:04.02 AS.seek 0.82ml
@03:23:07.32 delay 3 seconds
@03:23:10.34 ATV.dial PCR
@03:23:10.78 AS.reconfigure ASconfig
@03:23:10.83 AS.seek 0ml
@03:23:58.25 PCR.program :bleachProfile
@03:23:58.33 PCR program should complete in < 2:45
@03:23:58.34 PCR.run 10:00
@03:27:00.19 PCR.off
@03:27:00.23 AFV.select :carrierSolution
@03:27:00.34 delay 2 seconds
@03:27:02.36 AS.seek 1ml
@03:28:00.11 delay 1 second
@03:28:01.14 AFV.select :holdingCoil
@03:28:01.24 delay 1 second
@03:28:02.26 AS.reconfigure ASfast
@03:28:02.32 AS.seek 0ml
@03:28:29.68 delay 3 seconds
@03:28:32.70 AFV.select :carrierSolution
@03:28:32.80 delay 2 seconds
@03:28:34.82 AS.reconfigure ASconfig
@03:28:34.88 AS.seek 1ml
@03:29:32.67 delay 1 second
@03:29:33.70 AFV.select :holdingCoil
@03:29:33.80 delay 1 second
@03:29:34.82 AS.reconfigure ASfast
@03:29:34.88 AS.seek 0ml
@03:30:02.23 delay 3 seconds
@03:30:05.25 AFV.select :carrierSolution
@03:30:05.35 delay 2 seconds
@03:30:07.37 AS.reconfigure ASconfig
@03:30:07.43 AS.seek 1ml
@03:31:05.22 delay 1 second
@03:31:06.25 AFV.select :holdingCoil
@03:31:06.35 delay 1 second
@03:31:07.37 AS.reconfigure ASfast
@03:31:07.43 AS.seek 0ml
@03:31:34.78 delay 3 seconds
@03:31:37.80 AFV.select :carrierSolution
@03:31:37.90 delay 2 seconds
@03:31:39.92 AS.reconfigure ASconfig
@03:31:39.99 AS.seek 1ml
@03:32:37.75 delay 1 second
@03:32:38.78 AFV.select :holdingCoil
@03:32:38.88 delay 1 second
@03:32:39.90 AS.reconfigure ASfast
@03:32:39.96 AS.seek 0ml
@03:33:07.31 delay 3 seconds
@03:33:10.33 AFV.select :carrierSolution
@03:33:10.43 delay 2 seconds
@03:33:12.45 AS.reconfigure ASconfig
@03:33:12.51 AS.seek 1ml
@03:34:10.30 delay 1 second
@03:34:11.33 AFV.select :holdingCoil
@03:34:11.43 delay 1 second
@03:34:12.45 AS.reconfigure ASnorm
@03:34:12.51 AS.seek 0ml
@03:35:03.23 AFV.select :carrierSolution
@03:35:03.34 delay 2 seconds
@03:35:05.36 AS.reconfigure ASconfig
@03:35:05.41 AS.seek 1ml
@03:36:03.17 delay 1 second
@03:36:04.20 AFV.select :holdingCoil
@03:36:04.30 delay 1 second
@03:36:05.32 ATV.dial :waste
@03:36:06.68 AS.reconfigure ASnorm
@03:36:06.74 AS.seek 0.5ml
@03:36:32.28 ATV.dial :waste2
@03:36:33.64 AS.seek 0ml
@03:36:59.20 <MAIN> Cleaning PCR
@03:36:59.21 AFV.select :carrierSolution
@03:36:59.33 delay 2 seconds
@03:37:01.35 AS.reconfigure ASconfig
@03:37:01.41 AS.seek 0.5ml
@03:37:30.47 delay 1 second
@03:37:31.49 AFV.select :holdingCoil
@03:37:31.59 ASV.dial :waste1
@03:37:31.60 ATV.dial :air3
@03:37:33.66 AS.reconfigure ASslow1
@03:37:33.71 AS.seek 0.51ml
@03:37:35.98 delay 2 seconds
@03:37:38.00 ATV.dial :bleach
@03:37:38.48 AS.seek 0.81ml
@03:38:39.09 delay 3 seconds
@03:38:42.11 ATV.dial :air3
@03:38:42.72 delay 3 seconds
@03:38:45.79 AS.seek 0.835ml
@03:38:51.11 delay 3 seconds
@03:38:54.13 ATV.dial :bleach
@03:38:54.77 AS.seek 0.82ml
@03:38:58.07 delay 3 seconds
@03:39:01.09 ARV.dial :waste
@03:39:01.61 delay 3 seconds
@03:39:04.63 ATV.dial :ARV
@03:39:05.66 AS.reconfigure ASconfig
@03:39:05.71 AS.seek 0.47ml
@03:39:26.11 AFV.select :carrierSolution
@03:39:26.23 delay 2 seconds
@03:39:28.25 AS.seek 1ml
@03:39:59.00 delay 1 second
@03:40:00.02 AFV.select :holdingCoil
@03:40:00.12 delay 1 second
@03:40:01.14 AS.reconfigure ASfast
@03:40:01.20 AS.seek 0ml
@03:40:28.53 AFV.select :carrierSolution
@03:40:28.65 delay 2 seconds
@03:40:30.67 AS.reconfigure ASconfig
@03:40:30.72 AS.seek 1ml
@03:41:28.50 delay 1 second
@03:41:29.53 AFV.select :holdingCoil
@03:41:29.63 delay 1 second
@03:41:30.65 AS.reconfigure ASfast
@03:41:30.71 AS.seek 0ml
@03:41:58.03 AFV.select :carrierSolution
@03:41:58.15 delay 2 seconds
@03:42:00.17 AS.reconfigure ASconfig
@03:42:00.22 AS.seek 1ml
@03:42:57.96 delay 1 second
@03:42:58.99 AFV.select :holdingCoil
@03:42:59.09 delay 1 second
@03:43:00.11 AS.reconfigure ASfast
@03:43:00.16 AS.seek 0ml
@03:43:27.49 ARV.dial between carrier and air1
@03:43:28.03 ATV.dial :air3
@03:43:29.39 AS.reconfigure ASslow1
@03:43:29.45 AS.seek 0.01ml
@03:43:31.91 delay 2 seconds
@03:43:33.94 ATV.dial :bleach
@03:43:34.59 AS.seek 0.81ml
@03:46:15.72 delay 3 seconds
@03:46:18.75 ATV.dial :air3
@03:46:19.36 delay 3 seconds
@03:46:22.43 AS.seek 0.835ml
@03:46:27.70 delay 3 seconds
@03:46:30.73 ATV.dial :bleach
@03:46:31.39 AS.seek 0.82ml
@03:46:34.80 delay 3 seconds
@03:46:37.83 ATV.dial PCR
@03:46:38.25 AS.reconfigure ASconfig
@03:46:38.30 AS.seek 0ml
@03:47:25.74 PCR.program :bleachProfile
@03:47:25.82 PCR program should complete in < 2:45
@03:47:25.83 PCR.run 10:00
@03:50:28.07 PCR.off
@03:50:28.11 AFV.select :carrierSolution
@03:50:28.22 delay 2 seconds
@03:50:30.24 AS.seek 1ml
@03:51:28.01 delay 1 second
@03:51:29.03 AFV.select :holdingCoil
@03:51:29.13 delay 1 second
@03:51:30.15 AS.reconfigure ASfast
@03:51:30.21 AS.seek 0ml
@03:51:57.54 delay 3 seconds
@03:52:00.56 AFV.select :carrierSolution
@03:52:00.66 delay 2 seconds
@03:52:02.68 AS.reconfigure ASconfig
@03:52:02.77 AS.seek 1ml
@03:53:00.50 delay 1 second
@03:53:01.52 AFV.select :holdingCoil
@03:53:01.62 delay 1 second
@03:53:02.64 AS.reconfigure ASfast
@03:53:02.70 AS.seek 0ml
@03:53:30.03 delay 3 seconds
@03:53:33.05 AFV.select :carrierSolution
@03:53:33.15 delay 2 seconds
@03:53:35.17 AS.reconfigure ASconfig
@03:53:35.22 AS.seek 1ml
@03:54:33.00 delay 1 second
@03:54:34.03 AFV.select :holdingCoil
@03:54:34.13 delay 1 second
@03:54:35.15 AS.reconfigure ASfast
@03:54:35.20 AS.seek 0ml
@03:55:02.54 delay 3 seconds
@03:55:05.56 AFV.select :carrierSolution
@03:55:05.66 delay 2 seconds
@03:55:07.68 AS.reconfigure ASconfig
@03:55:07.74 AS.seek 1ml
@03:56:05.53 delay 1 second
@03:56:06.56 AFV.select :holdingCoil
@03:56:06.66 delay 1 second
@03:56:07.68 AS.reconfigure ASfast
@03:56:07.73 AS.seek 0ml
@03:56:35.07 delay 3 seconds
@03:56:38.09 AFV.select :carrierSolution
@03:56:38.19 delay 2 seconds
@03:56:40.21 AS.reconfigure ASconfig
@03:56:40.28 AS.seek 1ml
@03:57:38.07 delay 1 second
@03:57:39.09 AFV.select :holdingCoil
@03:57:39.19 delay 1 second
@03:57:40.21 AS.reconfigure ASnorm
@03:57:40.26 AS.seek 0ml
@03:58:30.95 delay 5 seconds
@03:58:36.02 Cleaning PCR
@03:58:36.03 AFV.select :carrierSolution
@03:58:36.15 delay 2 seconds
@03:58:38.17 AS.reconfigure ASconfig
@03:58:38.22 AS.seek 0.5ml
@03:59:07.28 delay 1 second
@03:59:08.31 AFV.select :holdingCoil
@03:59:08.41 ASV.dial :waste1
@03:59:08.42 ATV.dial :air3
@03:59:09.31 AS.reconfigure ASslow1
@03:59:09.36 AS.seek 0.51ml
@03:59:11.64 delay 2 seconds
@03:59:13.67 ATV.dial :bleach
@03:59:14.31 AS.seek 0.81ml
@04:00:14.89 delay 3 seconds
@04:00:17.92 ATV.dial :air3
@04:00:18.51 delay 3 seconds
@04:00:21.58 AS.seek 0.835ml
@04:00:26.86 delay 3 seconds
@04:00:29.89 ATV.dial :bleach
@04:00:30.53 AS.seek 0.82ml
@04:00:33.83 delay 3 seconds
@04:00:36.86 ARV.dial :waste
@04:00:37.39 delay 3 seconds
@04:00:40.41 ATV.dial :ARV
@04:00:41.33 AS.reconfigure ASconfig
@04:00:41.38 AS.seek 0.47ml
@04:01:01.78 AFV.select :carrierSolution
@04:01:01.90 delay 2 seconds
@04:01:03.92 AS.seek 1ml
@04:01:34.72 delay 1 second
@04:01:35.74 AFV.select :holdingCoil
@04:01:35.84 delay 1 second
@04:01:36.86 AS.reconfigure ASfast
@04:01:36.91 AS.seek 0ml
@04:02:04.25 AFV.select :carrierSolution
@04:02:04.37 delay 2 seconds
@04:02:06.39 AS.reconfigure ASconfig
@04:02:06.44 AS.seek 1ml
@04:03:04.22 delay 1 second
@04:03:05.25 AFV.select :holdingCoil
@04:03:05.35 delay 1 second
@04:03:06.37 AS.reconfigure ASfast
@04:03:06.43 AS.seek 0ml
@04:03:33.74 AFV.select :carrierSolution
@04:03:33.86 delay 2 seconds
@04:03:35.88 AS.reconfigure ASconfig
@04:03:35.93 AS.seek 1ml
@04:04:33.71 delay 1 second
@04:04:34.74 AFV.select :holdingCoil
@04:04:34.84 delay 1 second
@04:04:35.86 AS.reconfigure ASfast
@04:04:35.91 AS.seek 0ml
@04:05:03.25 ARV.dial between carrier and air1
@04:05:03.78 ATV.dial :air3
@04:05:05.11 AS.reconfigure ASslow1
@04:05:05.16 AS.seek 0.01ml
@04:05:07.66 delay 2 seconds
@04:05:09.69 ATV.dial :bleach
@04:05:10.33 AS.seek 0.81ml
@04:07:51.48 delay 3 seconds
@04:07:54.50 ATV.dial :air3
@04:07:55.11 delay 3 seconds
@04:07:58.18 AS.seek 0.835ml
@04:08:03.56 delay 3 seconds
@04:08:06.58 ATV.dial :bleach
@04:08:07.23 AS.seek 0.82ml
@04:08:10.66 delay 3 seconds
@04:08:13.69 ATV.dial PCR
@04:08:14.17 AS.reconfigure ASconfig
@04:08:14.22 AS.seek 0ml
@04:09:01.66 PCR.program :bleachProfile
@04:09:01.73 PCR program should complete in < 2:45
@04:09:01.74 PCR.run 10:00
@04:12:03.62 PCR.off
@04:12:03.66 AFV.select :carrierSolution
@04:12:03.77 delay 2 seconds
@04:12:05.79 AS.seek 1ml
@04:13:03.55 delay 1 second
@04:13:04.58 AFV.select :holdingCoil
@04:13:04.68 delay 1 second
@04:13:05.70 AS.reconfigure ASfast
@04:13:05.76 AS.seek 0ml
@04:13:33.11 delay 3 seconds
@04:13:36.13 AFV.select :carrierSolution
@04:13:36.23 delay 2 seconds
@04:13:38.25 AS.reconfigure ASconfig
@04:13:38.33 AS.seek 1ml
@04:14:36.11 delay 1 second
@04:14:37.14 AFV.select :holdingCoil
@04:14:37.24 delay 1 second
@04:14:38.26 AS.reconfigure ASfast
@04:14:38.32 AS.seek 0ml
@04:15:05.65 delay 3 seconds
@04:15:08.67 AFV.select :carrierSolution
@04:15:08.77 delay 2 seconds
@04:15:10.79 AS.reconfigure ASconfig
@04:15:10.85 AS.seek 1ml
@04:16:08.60 delay 1 second
@04:16:09.63 AFV.select :holdingCoil
@04:16:09.73 delay 1 second
@04:16:10.75 AS.reconfigure ASfast
@04:16:10.81 AS.seek 0ml
@04:16:38.17 delay 3 seconds
@04:16:41.19 AFV.select :carrierSolution
@04:16:41.29 delay 2 seconds
@04:16:43.31 AS.reconfigure ASconfig
@04:16:43.38 AS.seek 1ml
@04:17:41.13 delay 1 second
@04:17:42.16 AFV.select :holdingCoil
@04:17:42.26 delay 1 second
@04:17:43.28 AS.reconfigure ASfast
@04:17:43.34 AS.seek 0ml
@04:18:10.68 delay 3 seconds
@04:18:13.70 AFV.select :carrierSolution
@04:18:13.80 delay 2 seconds
@04:18:15.82 AS.reconfigure ASconfig
@04:18:15.88 AS.seek 1ml
@04:19:13.66 delay 1 second
@04:19:14.69 AFV.select :holdingCoil
@04:19:14.79 delay 1 second
@04:19:15.81 AS.reconfigure ASnorm
@04:19:15.86 AS.seek 0ml
@04:20:06.58 delay 5 seconds
@04:20:11.66 Cleaning PCR
@04:20:11.67 AFV.select :carrierSolution
@04:20:11.79 delay 2 seconds
@04:20:13.81 AS.reconfigure ASconfig
@04:20:13.86 AS.seek 0.5ml
@04:20:42.88 delay 1 second
@04:20:43.91 AFV.select :holdingCoil
@04:20:44.01 ASV.dial :waste1
@04:20:44.02 ATV.dial :air3
@04:20:44.92 AS.reconfigure ASslow1
@04:20:44.97 AS.seek 0.51ml
@04:20:47.26 delay 2 seconds
@04:20:49.28 ATV.dial :bleach
@04:20:49.92 AS.seek 0.81ml
@04:21:50.61 delay 3 seconds
@04:21:53.95 ATV.dial :air3
@04:21:54.56 delay 3 seconds
@04:21:57.63 AS.seek 0.835ml
@04:22:02.93 delay 3 seconds
@04:22:05.96 ATV.dial :bleach
@04:22:06.61 AS.seek 0.82ml
@04:22:09.93 delay 3 seconds
@04:22:12.95 ARV.dial :waste
@04:22:13.47 delay 3 seconds
@04:22:16.49 ATV.dial :ARV
@04:22:17.41 AS.reconfigure ASconfig
@04:22:17.46 AS.seek 0.47ml
@04:22:37.85 AFV.select :carrierSolution
@04:22:37.97 delay 2 seconds
@04:22:39.99 AS.seek 1ml
@04:23:10.76 delay 1 second
@04:23:11.78 AFV.select :holdingCoil
@04:23:11.88 delay 1 second
@04:23:12.90 AS.reconfigure ASfast
@04:23:12.96 AS.seek 0ml
@04:23:40.28 AFV.select :carrierSolution
@04:23:40.40 delay 2 seconds
@04:23:42.42 AS.reconfigure ASconfig
@04:23:42.47 AS.seek 1ml
@04:24:40.22 delay 1 second
@04:24:41.24 AFV.select :holdingCoil
@04:24:41.34 delay 1 second
@04:24:42.36 AS.reconfigure ASfast
@04:24:42.41 AS.seek 0ml
@04:25:09.74 AFV.select :carrierSolution
@04:25:09.86 delay 2 seconds
@04:25:11.88 AS.reconfigure ASconfig
@04:25:11.94 AS.seek 1ml
@04:26:09.71 delay 1 second
@04:26:10.74 AFV.select :holdingCoil
@04:26:10.84 delay 1 second
@04:26:11.86 AS.reconfigure ASfast
@04:26:11.91 AS.seek 0ml
@04:26:39.25 ARV.dial between carrier and air1
@04:26:39.78 ATV.dial :air3
@04:26:41.14 AS.reconfigure ASslow1
@04:26:41.19 AS.seek 0.01ml
@04:26:43.48 delay 2 seconds
@04:26:45.50 ATV.dial :bleach
@04:26:46.14 AS.seek 0.81ml
@04:29:27.27 delay 3 seconds
@04:29:30.30 ATV.dial :air3
@04:29:30.89 delay 3 seconds
@04:29:33.96 AS.seek 0.835ml
@04:29:39.25 delay 3 seconds
@04:29:42.28 ATV.dial :bleach
@04:29:42.92 AS.seek 0.82ml
@04:29:46.21 delay 3 seconds
@04:29:49.24 ATV.dial PCR
@04:29:49.65 AS.reconfigure ASconfig
@04:29:49.70 AS.seek 0ml
@04:30:37.11 PCR.program :bleachProfile
@04:30:37.18 PCR program should complete in < 2:45
@04:30:37.19 PCR.run 10:00
@04:33:39.05 PCR.off
@04:33:39.09 AFV.select :carrierSolution
@04:33:39.55 delay 2 seconds
@04:33:41.57 AS.seek 1ml
@04:34:39.38 delay 1 second
@04:34:40.41 AFV.select :holdingCoil
@04:34:40.51 delay 1 second
@04:34:41.53 AS.reconfigure ASfast
@04:34:41.59 AS.seek 0ml
@04:35:08.93 delay 3 seconds
@04:35:11.95 AFV.select :carrierSolution
@04:35:12.05 delay 2 seconds
@04:35:14.07 AS.reconfigure ASconfig
@04:35:14.13 AS.seek 1ml
@04:36:11.90 delay 1 second
@04:36:12.92 AFV.select :holdingCoil
@04:36:13.02 delay 1 second
@04:36:14.04 AS.reconfigure ASfast
@04:36:14.09 AS.seek 0ml
@04:36:41.43 delay 3 seconds
@04:36:44.45 AFV.select :carrierSolution
@04:36:44.55 delay 2 seconds
@04:36:46.57 AS.reconfigure ASconfig
@04:36:46.63 AS.seek 1ml
@04:37:44.39 delay 1 second
@04:37:45.41 AFV.select :holdingCoil
@04:37:45.51 delay 1 second
@04:37:46.53 AS.reconfigure ASfast
@04:37:46.59 AS.seek 0ml
@04:38:13.93 delay 3 seconds
@04:38:16.95 AFV.select :carrierSolution
@04:38:17.05 delay 2 seconds
@04:38:19.07 AS.reconfigure ASconfig
@04:38:19.12 AS.seek 1ml
@04:39:16.87 delay 1 second
@04:39:17.89 AFV.select :holdingCoil
@04:39:17.99 delay 1 second
@04:39:19.01 AS.reconfigure ASfast
@04:39:19.07 AS.seek 0ml
@04:39:46.42 delay 3 seconds
@04:39:49.44 AFV.select :carrierSolution
@04:39:49.54 delay 2 seconds
@04:39:51.56 AS.reconfigure ASconfig
@04:39:51.62 AS.seek 1ml
@04:40:49.42 delay 1 second
@04:40:50.44 AFV.select :holdingCoil
@04:40:50.54 delay 1 second
@04:40:51.56 AS.reconfigure ASnorm
@04:40:51.61 AS.seek 0ml
@04:41:42.33 delay 5 seconds
@04:41:47.41 <bkha> Session Ended
@16:41:13.17CEST19-Apr-21 <MAIN> ESP2 v3.30 I2C Gateway v3.06 for core bus
@16:41:15.42 
Configuration for mer 7/20/20 brent@mbari.org
/home/esp/esp2/type/shallow/mer/configure.rb
@16:41:15.60 
Configuration for MFB3 by preston@mbari.org
/home/esp/esp2/type/mfb/MFB3/configure.rb
@16:41:20.16 Polling Can every 10:00
@16:41:21.87 CS.configure :default
@16:41:21.91 CS.hold
@16:41:21.99 CS.configure :default
@16:41:22.15 PS.configure :default
@16:41:22.32 SS.configure :default
@16:41:22.39 SS.hold
@16:41:22.48 SS.configure :default
@16:41:22.63 CC.configure :default
@16:41:22.71 CC.hold
@16:41:22.78 CC.configure :default
@16:41:22.86 PC.configure :default
@16:41:22.90 PC.hold
@16:41:22.94 PC.configure :default
@16:41:23.04 Forearm.configure :loose
@16:41:23.08 Forearm.hold
@16:41:23.12 Forearm.configure :loose
@16:41:23.20 Elbow.configure :loose
@16:41:23.24 Elbow.hold
@16:41:23.28 Elbow.configure :loose
@16:41:23.38 SC.configure :loose
@16:41:23.42 SC.hold
@16:41:23.47 SC.configure :loose
@16:41:23.55 SE.configure :default
@16:41:23.59 SE.hold
@16:41:23.65 SE.configure :default
@16:41:23.69 CH.coast
@16:41:23.74 CH.configure :default
@16:41:23.76 PH.coast
@16:41:23.81 PH.configure :default
@16:41:23.91 SPE.coast
@16:41:23.95 SPE.configure SPEconfig
@16:41:24.03 AS.configure ASconfig
@16:41:24.06 AS.hold
@16:41:24.11 AS.configure ASconfig
@16:41:24.16 PCR.off
@16:41:24.19 PCR.pidtemp= 50,0.1,0,300,25,25
@16:41:24.22 PCR.caltemp= 2.5051,0.0372,0
@16:41:24.25 All dwarf controllers running firmware version 3.75
@16:41:24.46 Cleanly exited at 16:40:56CEST19-Apr-21
@16:41:24.49 Loading /home/esp/esp2/mission/GENOMAP_v3.rb mission
@16:41:24.63 <MFBPCR> Extracting Nucleic Acid from extSrc
@16:41:24.66 SPE.hold 2:46:40,55C
@16:41:24.70 ASV.dial :SPEdelivery
@16:41:25.14 delay 20 seconds
@16:41:25.95 <mwj> Session Begun
@16:41:45.18 <MFBPCR> AFV.select :holdingCoil
@16:41:45.30 ATV.dial :air2
@16:41:46.61 AS.reconfigure ASslow1
@16:41:46.67 AS.seek 0.07ml
@16:42:01.15 delay 3 seconds
@16:42:04.17 ATV.dial :extSrc
@16:42:05.70 AS.seek 0.47ml
@16:43:26.44 delay 5 seconds
@16:43:31.52 Solid Phase Extraction Heater at 40.5C
@16:43:31.53 ATV.dial ASV
@16:43:32.41 ASV.dial :SPEdelivery
@16:43:32.50 AS.seek 0.042ml
@16:44:58.87 delay 10 seconds
@16:45:08.90 Rinsing external lysate line extSrc
@16:45:08.91 AFV.select :carrierSolution
@16:45:09.03 delay 2 seconds
@16:45:11.05 AS.reconfigure ASconfig
@16:45:11.11 AS.seek 1ml
@16:46:06.47 delay 1 second
@16:46:07.50 AFV.select :holdingCoil
@16:46:07.60 delay 1 second
@16:46:08.62 ATV.dial :extSrc
@16:46:09.44 AS.reconfigure ASnorm
@16:46:09.49 AS.seek 0ml
@16:47:00.21 ATV.dial :air2
@16:47:01.81 AS.reconfigure ASslow0
@16:47:01.87 AS.seek 0.05ml
@16:47:07.28 ATV.dial :extSrc
@16:47:08.89 AS.seek 0.01ml
@16:47:13.22 ATV.dial :waste
@16:47:14.94 AS.reconfigure ASconfig
@16:47:15.04 AS.seek 0ml
@16:47:15.92 ethanol wash
@16:47:15.93 ATV.dial :air2
@16:47:17.14 AS.reconfigure ASslow2
@16:47:17.20 AS.seek 0.01ml
@16:47:27.64 delay 3 seconds
@16:47:30.66 Pulling 0.4ml of Ethanol Wash
@16:47:30.67 ATV.dial :ethanol
@16:47:31.55 AS.reconfigure ASslow1
@16:47:31.61 AS.seek 0.41ml
@16:48:52.46 delay 3 seconds
@16:48:55.49 ATV.dial :air1
@16:48:55.94 delay 3 seconds
@16:48:59.01 AS.seek 0.435ml
@16:49:04.41 delay 3 seconds
@16:49:07.44 ATV.dial :ethanol
@16:49:08.08 AS.seek 0.42ml
@16:49:11.36 delay 3 seconds
@16:49:14.39 ATV.dial ASV
@16:49:15.78 AS.seek 0.093ml
@16:50:21.85 delay 3 seconds
@16:50:24.88 ATV.dial :waste2
@16:50:25.69 AS.reconfigure ASconfig
@16:50:25.74 AS.seek 0ml
@16:50:31.38 ATV.dial :air2
@16:50:31.81 AS.reconfigure ASslow1
@16:50:31.86 AS.seek 1ml
@16:53:53.28 delay 5 seconds
@16:53:58.30 ATV.dial ASV
@16:53:59.34 ASV.dial :SPEdelivery
@16:53:59.36 AS.seek 0.02ml
@16:57:16.72 delay 5 seconds
@16:57:21.75 ATV.dial :air2
@16:57:22.84 AS.seek 0.52ml
@16:59:03.71 delay 5 seconds
@16:59:08.74 ATV.dial ASV
@16:59:09.78 ASV.dial :wasteR
@16:59:10.17 AS.seek 0.3ml
@16:59:54.78 delay 20 seconds
@17:00:14.81 AS.seek 0.07ml
@17:01:01.28 delay 20 seconds
@17:01:21.31 ATV.dial :waste2
@17:01:22.12 AS.reconfigure ASconfig
@17:01:22.18 AS.seek 0ml
@17:01:26.51 Elution
@17:01:26.52 ATV.dial :air1
@17:01:27.72 AS.reconfigure ASslow1
@17:01:27.78 AS.seek 0.05ml
@17:01:38.11 delay 3 seconds
@17:01:41.14 ATV.dial :ElutionWater
@17:01:41.84 AS.seek 0.11ml
@17:01:54.17 delay 3 seconds
@17:01:57.20 ATV.dial ASV,via: :air2
@17:01:59.44 ASV.dial :SPEelution
@17:02:00.07 AFV.select :carrierSolution
@17:02:00.23 AS.reconfigure ASnorm
@17:02:00.29 AS.seek 0.76ml
@17:02:33.33 delay 3 seconds
@17:02:36.36 AFV.select :holdingCoil
@17:02:36.50 AS.reconfigure ASslow1
@17:02:36.56 AS.seek 0.475ml
@17:03:34.18 delay 5 seconds
@17:03:39.25 AS.reconfigure ASslow2
@17:03:39.31 AS.seek 0.44ml
@17:04:14.96 delay 1:00
@17:05:15.00 pushing eluate to holding coil
@17:05:15.07 AS.seek 0.28ml
@17:07:56.58 delay 30 seconds
@17:08:26.62 ATV.dial :waste,via: :air3
@17:08:28.47 AS.reconfigure ASconfig
@17:08:28.53 AS.seek 0ml
@17:08:44.90 Parking Bleach in LysateSource line and SPEcolumn
@17:08:44.91 ATV.dial :air3
@17:08:45.81 AS.reconfigure ASslow1
@17:08:45.86 AS.seek 0.03ml
@17:08:52.28 ATV.dial :ElutionWater
@17:08:52.92 AS.seek 0.06ml
@17:08:59.35 ATV.dial :air3
@17:09:00.00 AS.seek 0.07ml
@17:09:02.30 delay 2 seconds
@17:09:04.33 ATV.dial :bleach
@17:09:04.83 AS.seek 0.15ml
@17:09:21.14 delay 3 seconds
@17:09:24.17 ATV.dial :air3
@17:09:24.75 delay 3 seconds
@17:09:27.82 AS.seek 0.175ml
@17:09:33.10 delay 3 seconds
@17:09:36.13 ATV.dial :bleach
@17:09:36.77 AS.seek 0.16ml
@17:09:40.17 delay 3 seconds
@17:09:43.20 ATV.dial :extSrc
@17:09:43.84 AS.reconfigure ASconfig
@17:09:43.89 AS.seek 0.015ml
@17:09:52.53 ATV.dial :waste
@17:09:54.08 AS.seek 0ml
@17:09:55.24 ATV.dial :air3
@17:09:56.14 AS.reconfigure ASslow1
@17:09:56.20 AS.seek 0.01ml
@17:09:58.61 delay 2 seconds
@17:10:00.64 ATV.dial :bleach
@17:10:01.13 AS.seek 0.58ml
@17:11:56.07 delay 3 seconds
@17:11:59.09 ATV.dial :air3
@17:11:59.67 delay 3 seconds
@17:12:02.74 AS.seek 0.605ml
@17:12:08.65 delay 3 seconds
@17:12:11.68 ATV.dial :bleach
@17:12:12.31 AS.seek 0.59ml
@17:12:15.75 delay 3 seconds
@17:12:18.78 ATV.dial :ASV
@17:12:19.88 ASV.dial :wasteR
@17:12:20.51 AS.seek 0ml
@17:14:19.50 delay 1:00
@17:15:19.53 AFV.select :carrierSolution
@17:15:19.63 delay 2 seconds
@17:15:21.65 AS.reconfigure ASconfig
@17:15:21.70 AS.seek 1ml
@17:16:19.47 delay 1 second
@17:16:20.50 AFV.select :holdingCoil
@17:16:20.60 delay 1 second
@17:16:21.62 ATV.dial ASV
@17:16:21.63 ASV.dial :wasteR
@17:16:21.66 AS.reconfigure ASslow1
@17:16:21.72 AS.seek 0ml
@17:19:43.12 delay 20 seconds
@17:20:03.15 ATV.dial :air3
@17:20:04.75 AS.seek 0.59ml
@17:22:03.78 delay 3 seconds
@17:22:06.80 ATV.dial :ASV
@17:22:08.31 ASV.dial :wasteR
@17:22:08.40 AS.seek 0.09ml
@17:23:49.24 delay 30 seconds
@17:24:19.28 ATV.dial :extSrc
@17:24:20.11 delay 3 seconds
@17:24:23.13 ATV.dial :ElutionWater
@17:24:24.32 AS.seek 0.12ml
@17:24:30.61 ATV.dial :air3
@17:24:31.27 AS.seek 0.13ml
@17:24:33.55 delay 2 seconds
@17:24:35.58 ATV.dial :bleach
@17:24:36.07 AS.seek 0.23ml
@17:24:56.42 delay 3 seconds
@17:24:59.45 ATV.dial :air3
@17:25:00.03 delay 3 seconds
@17:25:03.10 AS.seek 0.255ml
@17:25:08.42 delay 3 seconds
@17:25:11.45 ATV.dial :bleach
@17:25:12.52 AS.seek 0.24ml
@17:25:15.88 delay 3 seconds
@17:25:18.91 ATV.dial :ASV
@17:25:20.02 ASV.dial :SPEdelivery
@17:25:20.42 AFV.select :carrierSolution
@17:25:20.54 delay 2 seconds
@17:25:22.56 AS.reconfigure ASconfig
@17:25:22.61 AS.seek 0.5ml
@17:25:37.81 delay 1 second
@17:25:38.84 AFV.select :holdingCoil
@17:25:39.00 AS.reconfigure ASslow1
@17:25:39.06 AS.seek 0.17ml
@17:26:45.71 SPE.hold 2:46:40,95C
@17:26:45.76 delay 3:00
@17:29:45.81 AFV.select :carrierSolution
@17:29:45.92 delay 2 seconds
@17:29:47.94 AS.reconfigure ASconfig
@17:29:47.99 AS.seek 1ml
@17:30:36.00 delay 1 second
@17:30:37.02 AFV.select :holdingCoil
@17:30:37.12 delay 1 second
@17:30:38.14 ASV.dial :wasteD
@17:30:39.28 AS.seek 0ml
@17:31:37.07 Priming Eluate
@17:31:37.08 AFV.select :carrierSolution
@17:31:37.20 delay 2 seconds
@17:31:39.22 AS.seek 0.5ml
@17:32:08.23 delay 1 second
@17:32:09.25 AFV.select :holdingCoil
@17:32:09.35 ATV.dial ASV
@17:32:09.36 ASV.dial :SPErecovery
@17:32:10.00 Swish #1 of 2
@17:32:10.09 AS.reconfigure ASslow0
@17:32:10.14 AS.seek 0.6ml
@17:32:20.58 AS.seek 0.5ml
@17:32:31.00 Swish #2 of 2
@17:32:31.09 AS.seek 0.6ml
@17:32:41.48 AS.seek 0.5ml
@17:32:51.89 delay 3 seconds
@17:32:54.95 AS.reconfigure ASslow1
@17:32:55.01 AS.seek 0.684ml
@17:33:32.32 delay 3 seconds
@17:33:35.34 Eluate primed
@17:33:35.35 ATV.dial :waste2
@17:33:36.03 AS.reconfigure ASnorm
@17:33:36.08 AS.seek 0ml
@17:34:10.86 SPE.coast
@17:34:10.94 ASV.dial :SPErecovery
@17:34:10.97 Setting up PCR for SYBRIPc -- writing /var/log/esp/SYBRIPc21apr1917h.pcr
@17:34:10.99 Building PCR Slug from ASV with MM1 mix, PP6 primer
@17:34:11.01 Priming with carrier
@17:34:11.02 ATV.dial ARV
@17:34:12.07 ARV.dial :carrier
@17:34:12.43 AS.seek 0.5ml
@17:34:38.11 AFV.select :carrierSolution
@17:34:38.23 delay 2 seconds
@17:34:40.25 AS.reconfigure ASconfig
@17:34:40.32 AS.seek 1ml
@17:35:09.34 delay 1 second
@17:35:10.36 AFV.select :holdingCoil
@17:35:10.46 delay 1 second
@17:35:11.48 ATV.dial :waste
@17:35:13.09 AS.reconfigure ASnorm
@17:35:13.15 AS.seek 0ml
@17:36:03.85 AFV.select :carrierSolution
@17:36:03.97 delay 2 seconds
@17:36:05.99 AS.reconfigure ASconfig
@17:36:06.05 AS.seek 0.5ml
@17:36:35.12 delay 1 second
@17:36:36.14 AFV.select :holdingCoil
@17:36:36.24 Building Slug
@17:36:36.25 ATV.dial :ARV
@17:36:38.03 delay 2 seconds
@17:36:40.05 ARV.dial :air1
@17:36:40.46 delay 2 seconds
@17:36:42.53 AS.reconfigure ASslow2
@17:36:42.60 AS.seek 0.506ml
@17:36:48.79 delay 2 seconds
@17:36:50.81 ARV.dial :MM1
@17:36:51.48 delay 2 seconds
@17:36:53.50 Adding MM1
@17:36:53.57 AS.seek 0.524ml
@17:37:12.07 delay 25 seconds
@17:37:37.09 ARV.dial :PP6
@17:37:38.46 Adding PP6
@17:37:38.47 delay 2 seconds
@17:37:40.55 AS.seek 0.53ml
@17:37:46.79 delay 10 seconds
@17:37:56.82 ARV.dial :air1
@17:37:57.70 adding ARV air
@17:37:57.71 delay 2 seconds
@17:37:59.79 AS.seek 0.536ml
@17:38:06.17 delay 5 seconds
@17:38:11.19 ARV.dial :carrier
@17:38:11.61 delay 7 seconds
@17:38:18.69 AS.seek 0.594ml
@17:39:17.32 delay 5 seconds
@17:39:22.34 ATV.dial :ASV
@17:39:22.78 delay 2 seconds
@17:39:24.85 AS.seek 0.6ml
@17:39:31.36 delay 4 seconds
@17:39:35.38 ATV.dial ARV
@17:39:35.98 delay 2 seconds
@17:39:38.00 ARV.dial :carrier
@17:39:38.01 delay 2 seconds
@17:39:40.09 AS.seek 0.65ml
@17:40:30.70 delay 4 seconds
@17:40:34.72 ATV.dial PCR
@17:40:35.37 ASV.dial :waste1
@17:40:36.20 ARV.dial :air1
@17:40:36.61 Positioning Slug by 0.324ml
@17:40:36.70 AS.seek 0.326ml
@17:46:02.84 delay 5 seconds
@17:46:07.86 ATV.dial ARV
@17:46:08.67 ASV.dial :PCRlock
@17:46:09.06 delay 5 seconds
@17:46:14.08 Running SYBRIPc Profile60CIQ1 thermocycling
@17:46:14.10 <PCR> PCR.program Profile60CIQ1
@17:46:14.22 Profile60CIQ1 should complete in < 1:56:15
@17:46:14.23 PCR.run 2:30:18.75
@19:26:02.78 PCR.off
@19:26:03.34 <MFBPCR> PCR for SYBRIPc completed -- closed /var/log/esp/SYBRIPc21apr1917h.pcr
@19:26:03.41 Cleaning PCR
@19:26:03.43 AFV.select :carrierSolution
@19:26:03.55 delay 2 seconds
@19:26:05.57 AS.reconfigure ASconfig
@19:26:05.62 AS.seek 0.5ml
@19:26:15.90 delay 1 second
@19:26:16.93 AFV.select :holdingCoil
@19:26:17.03 ASV.dial :waste1
@19:26:17.43 ATV.dial :air3
@19:26:18.78 AS.reconfigure ASslow1
@19:26:18.84 AS.seek 0.51ml
@19:26:21.15 delay 2 seconds
@19:26:23.18 ATV.dial :bleach
@19:26:23.82 AS.seek 0.81ml
@19:27:24.45 delay 3 seconds
@19:27:27.47 ATV.dial :air3
@19:27:28.06 delay 3 seconds
@19:27:31.13 AS.seek 0.835ml
@19:27:36.42 delay 3 seconds
@19:27:39.44 ATV.dial :bleach
@19:27:40.07 AS.seek 0.82ml
@19:27:43.37 delay 3 seconds
@19:27:46.40 ARV.dial :waste
@19:27:47.04 delay 3 seconds
@19:27:50.06 ATV.dial :ARV
@19:27:50.98 AS.reconfigure ASconfig
@19:27:51.03 AS.seek 0.47ml
@19:28:11.84 AFV.select :carrierSolution
@19:28:11.96 delay 2 seconds
@19:28:13.98 AS.seek 1ml
@19:28:44.73 delay 1 second
@19:28:45.75 AFV.select :holdingCoil
@19:28:45.85 delay 1 second
@19:28:46.87 AS.reconfigure ASfast
@19:28:46.93 AS.seek 0ml
@19:29:14.26 AFV.select :carrierSolution
@19:29:14.37 delay 2 seconds
@19:29:16.39 AS.reconfigure ASconfig
@19:29:16.45 AS.seek 1ml
@19:30:14.20 delay 1 second
@19:30:15.23 AFV.select :holdingCoil
@19:30:15.33 delay 1 second
@19:30:16.35 AS.reconfigure ASfast
@19:30:16.41 AS.seek 0ml
@19:30:43.76 AFV.select :carrierSolution
@19:30:43.88 delay 2 seconds
@19:30:45.90 AS.reconfigure ASconfig
@19:30:45.96 AS.seek 1ml
@19:31:43.69 delay 1 second
@19:31:44.71 AFV.select :holdingCoil
@19:31:44.81 delay 1 second
@19:31:45.83 AS.reconfigure ASfast
@19:31:45.88 AS.seek 0ml
@19:32:13.22 ARV.dial between carrier and air1
@19:32:13.76 ATV.dial :air3
@19:32:15.10 AS.reconfigure ASslow1
@19:32:15.16 AS.seek 0.01ml
@19:32:17.47 delay 2 seconds
@19:32:19.50 ATV.dial :bleach
@19:32:20.14 AS.seek 0.81ml
@19:35:01.36 delay 3 seconds
@19:35:04.39 ATV.dial :air3
@19:35:04.98 delay 3 seconds
@19:35:08.05 AS.seek 0.835ml
@19:35:13.33 delay 3 seconds
@19:35:16.36 ATV.dial :bleach
@19:35:16.99 AS.seek 0.82ml
@19:35:20.29 delay 3 seconds
@19:35:23.32 ATV.dial PCR
@19:35:23.74 AS.reconfigure ASconfig
@19:35:23.79 AS.seek 0ml
@19:36:11.20 PCR.program :bleachProfile
@19:36:11.28 PCR program should complete in < 2:45
@19:36:11.29 PCR.run 10:00
@19:39:07.08 PCR.off
@19:39:07.12 AFV.select :carrierSolution
@19:39:07.23 delay 2 seconds
@19:39:09.25 AS.seek 1ml
@19:40:07.01 delay 1 second
@19:40:08.04 AFV.select :holdingCoil
@19:40:08.14 delay 1 second
@19:40:09.16 AS.reconfigure ASfast
@19:40:09.21 AS.seek 0ml
@19:40:36.54 delay 3 seconds
@19:40:39.57 AFV.select :carrierSolution
@19:40:39.67 delay 2 seconds
@19:40:41.69 AS.reconfigure ASconfig
@19:40:41.75 AS.seek 1ml
@19:41:39.50 delay 1 second
@19:41:40.53 AFV.select :holdingCoil
@19:41:40.63 delay 1 second
@19:41:41.65 AS.reconfigure ASfast
@19:41:41.70 AS.seek 0ml
@19:42:09.07 delay 3 seconds
@19:42:12.09 AFV.select :carrierSolution
@19:42:12.19 delay 2 seconds
@19:42:14.21 AS.reconfigure ASconfig
@19:42:14.27 AS.seek 1ml
@19:43:12.06 delay 1 second
@19:43:13.09 AFV.select :holdingCoil
@19:43:13.19 delay 1 second
@19:43:14.21 AS.reconfigure ASfast
@19:43:14.26 AS.seek 0ml
@19:43:41.61 delay 3 seconds
@19:43:44.64 AFV.select :carrierSolution
@19:43:44.74 delay 2 seconds
@19:43:46.76 AS.reconfigure ASconfig
@19:43:46.82 AS.seek 1ml
@19:44:44.61 delay 1 second
@19:44:45.64 AFV.select :holdingCoil
@19:44:45.74 delay 1 second
@19:44:46.76 AS.reconfigure ASfast
@19:44:46.81 AS.seek 0ml
@19:45:14.14 delay 3 seconds
@19:45:17.17 AFV.select :carrierSolution
@19:45:17.27 delay 2 seconds
@19:45:19.29 AS.reconfigure ASconfig
@19:45:19.35 AS.seek 1ml
@19:46:17.12 delay 1 second
@19:46:18.15 AFV.select :holdingCoil
@19:46:18.25 delay 1 second
@19:46:19.27 AS.reconfigure ASnorm
@19:46:19.32 AS.seek 0ml
@19:47:10.01 Setting up PCR for ACYPP2 -- writing /var/log/esp/ACYPP221apr1919h.pcr
@19:47:10.03 Building PCR Slug from ASV with MM1 mix, PP2 primer
@19:47:10.05 Priming with carrier
@19:47:10.43 ATV.dial ARV
@19:47:11.16 ARV.dial :carrier
@19:47:11.49 AS.seek 0.5ml
@19:47:37.15 AFV.select :carrierSolution
@19:47:37.28 delay 2 seconds
@19:47:39.30 AS.reconfigure ASconfig
@19:47:39.37 AS.seek 1ml
@19:48:08.38 delay 1 second
@19:48:09.40 AFV.select :holdingCoil
@19:48:09.50 delay 1 second
@19:48:10.52 ATV.dial :waste
@19:48:12.29 AS.reconfigure ASnorm
@19:48:12.35 AS.seek 0ml
@19:49:03.03 AFV.select :carrierSolution
@19:49:03.16 delay 2 seconds
@19:49:05.18 AS.reconfigure ASconfig
@19:49:05.24 AS.seek 0.5ml
@19:49:34.32 delay 1 second
@19:49:35.34 AFV.select :holdingCoil
@19:49:35.44 Building Slug
@19:49:35.45 ATV.dial :ARV
@19:49:37.21 delay 2 seconds
@19:49:39.23 ARV.dial :air1
@19:49:39.64 delay 2 seconds
@19:49:41.71 AS.reconfigure ASslow2
@19:49:41.76 AS.seek 0.506ml
@19:49:47.97 delay 2 seconds
@19:49:50.00 ARV.dial :MM1
@19:49:50.66 delay 2 seconds
@19:49:52.68 Adding MM1
@19:49:52.76 AS.seek 0.524ml
@19:50:11.15 delay 25 seconds
@19:50:36.18 ARV.dial :PP2
@19:50:36.60 Adding PP2
@19:50:36.61 delay 2 seconds
@19:50:38.69 AS.seek 0.53ml
@19:50:44.98 delay 10 seconds
@19:50:55.01 ARV.dial :air2
@19:50:55.43 adding ARV air
@19:50:55.44 delay 2 seconds
@19:50:57.52 AS.seek 0.536ml
@19:51:04.19 delay 5 seconds
@19:51:09.21 ARV.dial :carrier
@19:51:10.57 delay 7 seconds
@19:51:17.65 AS.seek 0.594ml
@19:52:16.66 delay 5 seconds
@19:52:21.68 ATV.dial :ASV
@19:52:22.12 delay 2 seconds
@19:52:24.19 AS.seek 0.6ml
@19:52:30.46 delay 4 seconds
@19:52:34.48 ATV.dial ARV
@19:52:35.05 delay 2 seconds
@19:52:37.07 ARV.dial :carrier
@19:52:37.08 delay 2 seconds
@19:52:39.16 AS.seek 0.65ml
@19:53:29.93 delay 4 seconds
@19:53:33.96 ATV.dial PCR
@19:53:34.61 ASV.dial :waste1
@19:53:34.63 ARV.dial :air1
@19:53:35.05 Positioning Slug by 0.324ml
@19:53:35.13 AS.seek 0.326ml
@19:59:01.53 delay 5 seconds
@19:59:06.56 ATV.dial ARV
@19:59:07.37 ASV.dial :PCRlock
@19:59:07.79 delay 5 seconds
@19:59:12.81 Running ACYPP2 Profile60CIQ2 thermocycling
@19:59:12.83 <PCR> PCR.program Profile60CIQ2
@19:59:12.94 Profile60CIQ2 should complete in < 1:47:50
@19:59:12.95 PCR.run 2:19:47.5
@21:28:11.51 PCR.off
@21:28:12.13 <MFBPCR> Running ACYPP2 MeltProfile60 thermocycling
@21:28:12.15 <PCR> PCR.program MeltProfile60
@21:28:12.25 MeltProfile60 should complete in < 11:55
@21:28:12.26 PCR.run 19:53.75
@21:36:05.80 PCR.off
@21:36:06.72 <MFBPCR> PCR for ACYPP2 completed -- closed /var/log/esp/ACYPP221apr1919h.pcr
@21:36:06.79 Cleaning PCR
@21:36:06.80 AFV.select :carrierSolution
@21:36:06.92 delay 2 seconds
@21:36:08.94 AS.reconfigure ASconfig
@21:36:09.00 AS.seek 0.5ml
@21:36:19.31 delay 1 second
@21:36:20.34 AFV.select :holdingCoil
@21:36:20.44 ASV.dial :waste1
@21:36:20.83 ATV.dial :air3
@21:36:22.18 AS.reconfigure ASslow1
@21:36:22.24 AS.seek 0.51ml
@21:36:24.55 delay 2 seconds
@21:36:26.58 ATV.dial :bleach
@21:36:27.23 AS.seek 0.81ml
@21:37:27.82 delay 3 seconds
@21:37:30.85 ATV.dial :air3
@21:37:31.44 delay 3 seconds
@21:37:34.51 AS.seek 0.835ml
@21:37:39.79 delay 3 seconds
@21:37:42.82 ATV.dial :bleach
@21:37:43.46 AS.seek 0.82ml
@21:37:46.90 delay 3 seconds
@21:37:49.93 ARV.dial :waste
@21:37:50.57 delay 3 seconds
@21:37:53.59 ATV.dial :ARV
@21:37:54.51 AS.reconfigure ASconfig
@21:37:54.56 AS.seek 0.47ml
@21:38:14.99 AFV.select :carrierSolution
@21:38:15.11 delay 2 seconds
@21:38:17.13 AS.seek 1ml
@21:38:47.87 delay 1 second
@21:38:48.90 AFV.select :holdingCoil
@21:38:49.00 delay 1 second
@21:38:50.02 AS.reconfigure ASfast
@21:38:50.08 AS.seek 0ml
@21:39:17.43 AFV.select :carrierSolution
@21:39:17.55 delay 2 seconds
@21:39:19.57 AS.reconfigure ASconfig
@21:39:19.62 AS.seek 1ml
@21:40:17.40 delay 1 second
@21:40:18.43 AFV.select :holdingCoil
@21:40:18.53 delay 1 second
@21:40:19.55 AS.reconfigure ASfast
@21:40:19.61 AS.seek 0ml
@21:40:46.92 AFV.select :carrierSolution
@21:40:47.04 delay 2 seconds
@21:40:49.06 AS.reconfigure ASconfig
@21:40:49.11 AS.seek 1ml
@21:41:46.87 delay 1 second
@21:41:47.90 AFV.select :holdingCoil
@21:41:48.00 delay 1 second
@21:41:49.02 AS.reconfigure ASfast
@21:41:49.08 AS.seek 0ml
@21:42:16.40 ARV.dial between carrier and air1
@21:42:16.94 ATV.dial :air3
@21:42:18.29 AS.reconfigure ASslow1
@21:42:18.35 AS.seek 0.01ml
@21:42:20.88 delay 2 seconds
@21:42:22.91 ATV.dial :bleach
@21:42:23.55 AS.seek 0.81ml
@21:45:04.68 delay 3 seconds
@21:45:07.71 ATV.dial :air3
@21:45:08.30 delay 3 seconds
@21:45:11.36 AS.seek 0.835ml
@21:45:16.67 delay 3 seconds
@21:45:19.69 ATV.dial :bleach
@21:45:20.34 AS.seek 0.82ml
@21:45:23.65 delay 3 seconds
@21:45:26.68 ATV.dial PCR
@21:45:27.10 AS.reconfigure ASconfig
@21:45:27.15 AS.seek 0ml
@21:46:14.57 PCR.program :bleachProfile
@21:46:14.64 PCR program should complete in < 2:45
@21:46:14.65 PCR.run 10:00
@21:49:10.85 PCR.off
@21:49:10.90 AFV.select :carrierSolution
@21:49:11.01 delay 2 seconds
@21:49:13.03 AS.seek 1ml
@21:50:10.79 delay 1 second
@21:50:11.82 AFV.select :holdingCoil
@21:50:11.92 delay 1 second
@21:50:12.94 AS.reconfigure ASfast
@21:50:13.00 AS.seek 0ml
@21:50:40.34 delay 3 seconds
@21:50:43.37 AFV.select :carrierSolution
@21:50:43.47 delay 2 seconds
@21:50:45.49 AS.reconfigure ASconfig
@21:50:45.56 AS.seek 1ml
@21:51:43.35 delay 1 second
@21:51:44.37 AFV.select :holdingCoil
@21:51:44.47 delay 1 second
@21:51:45.49 AS.reconfigure ASfast
@21:51:45.55 AS.seek 0ml
@21:52:12.89 delay 3 seconds
@21:52:15.91 AFV.select :carrierSolution
@21:52:16.01 delay 2 seconds
@21:52:18.03 AS.reconfigure ASconfig
@21:52:18.13 AS.seek 1ml
@21:53:15.92 delay 1 second
@21:53:16.95 AFV.select :holdingCoil
@21:53:17.05 delay 1 second
@21:53:18.07 AS.reconfigure ASfast
@21:53:18.13 AS.seek 0ml
@21:53:45.46 delay 3 seconds
@21:53:48.49 AFV.select :carrierSolution
@21:53:48.59 delay 2 seconds
@21:53:50.61 AS.reconfigure ASconfig
@21:53:50.67 AS.seek 1ml
@21:54:48.46 delay 1 second
@21:54:49.49 AFV.select :holdingCoil
@21:54:49.59 delay 1 second
@21:54:50.61 AS.reconfigure ASfast
@21:54:50.67 AS.seek 0ml
@21:55:17.99 delay 3 seconds
@21:55:21.02 AFV.select :carrierSolution
@21:55:21.12 delay 2 seconds
@21:55:23.14 AS.reconfigure ASconfig
@21:55:23.19 AS.seek 1ml
@21:56:20.98 delay 1 second
@21:56:22.01 AFV.select :holdingCoil
@21:56:22.11 delay 1 second
@21:56:23.13 AS.reconfigure ASnorm
@21:56:23.18 AS.seek 0ml
@21:57:13.90 Setting up PCR for AOPP1 -- writing /var/log/esp/AOPP121apr1921h.pcr
@21:57:13.92 Building PCR Slug from ASV with MM1 mix, PP1 primer
@21:57:13.94 Priming with carrier
@21:57:13.96 ATV.dial ARV
@21:57:14.62 ARV.dial :carrier
@21:57:14.98 AS.seek 0.5ml
@21:57:40.51 AFV.select :carrierSolution
@21:57:40.63 delay 2 seconds
@21:57:42.65 AS.reconfigure ASconfig
@21:57:42.71 AS.seek 1ml
@21:58:11.73 delay 1 second
@21:58:12.76 AFV.select :holdingCoil
@21:58:12.86 delay 1 second
@21:58:13.88 ATV.dial :waste
@21:58:15.64 AS.reconfigure ASnorm
@21:58:15.70 AS.seek 0ml
@21:59:06.40 AFV.select :carrierSolution
@21:59:06.52 delay 2 seconds
@21:59:08.54 AS.reconfigure ASconfig
@21:59:08.60 AS.seek 0.5ml
@21:59:37.67 delay 1 second
@21:59:38.70 AFV.select :holdingCoil
@21:59:38.82 Building Slug
@21:59:38.83 ATV.dial :ARV
@21:59:40.58 delay 2 seconds
@21:59:42.60 ARV.dial :air1
@21:59:43.00 delay 2 seconds
@21:59:45.08 AS.reconfigure ASslow2
@21:59:45.14 AS.seek 0.506ml
@21:59:51.34 delay 2 seconds
@21:59:53.37 ARV.dial :MM1
@21:59:54.00 delay 2 seconds
@21:59:56.02 Adding MM1
@21:59:56.09 AS.seek 0.524ml
@22:00:14.31 delay 25 seconds
@22:00:39.34 ARV.dial :PP1
@22:00:39.75 Adding PP1
@22:00:39.76 delay 2 seconds
@22:00:41.84 AS.seek 0.53ml
@22:00:48.39 delay 10 seconds
@22:00:58.42 ARV.dial :air1
@22:00:58.85 adding ARV air
@22:00:58.86 delay 2 seconds
@22:01:00.94 AS.seek 0.536ml
@22:01:07.35 delay 5 seconds
@22:01:12.38 ARV.dial :carrier
@22:01:12.82 delay 7 seconds
@22:01:19.90 AS.seek 0.594ml
@22:02:19.15 delay 5 seconds
@22:02:24.18 ATV.dial :ASV
@22:02:24.61 delay 2 seconds
@22:02:26.68 AS.seek 0.6ml
@22:02:33.28 delay 4 seconds
@22:02:37.30 ATV.dial ARV
@22:02:37.88 delay 2 seconds
@22:02:39.90 ARV.dial :carrier
@22:02:39.91 delay 2 seconds
@22:02:41.99 AS.seek 0.65ml
@22:03:32.60 delay 4 seconds
@22:03:36.62 ATV.dial PCR
@22:03:37.27 ASV.dial :waste1
@22:03:37.28 ARV.dial :air1
@22:03:37.70 Positioning Slug by 0.324ml
@22:03:37.79 AS.seek 0.326ml
@22:09:04.33 delay 5 seconds
@22:09:09.35 ATV.dial ARV
@22:09:10.17 ASV.dial :PCRlock
@22:09:10.58 delay 5 seconds
@22:09:15.60 Running AOPP1 Profile60CIQ1 thermocycling
@22:09:15.61 <PCR> PCR.program Profile60CIQ1
@22:09:15.73 Profile60CIQ1 should complete in < 1:56:15
@22:09:15.74 PCR.run 2:30:18.75
@23:48:52.36 PCR.off
@23:48:52.91 <MFBPCR> Running AOPP1 MeltProfile60 thermocycling
@23:48:52.93 <PCR> PCR.program MeltProfile60
@23:48:53.03 MeltProfile60 should complete in < 11:55
@23:48:53.04 PCR.run 19:53.75
@23:56:31.25 PCR.off
@23:56:32.18 <MFBPCR> PCR for AOPP1 completed -- closed /var/log/esp/AOPP121apr1921h.pcr
@23:56:32.25 Cleaning PCR
@23:56:32.26 AFV.select :carrierSolution
@23:56:32.38 delay 2 seconds
@23:56:34.40 AS.reconfigure ASconfig
@23:56:34.45 AS.seek 0.5ml
@23:56:44.74 delay 1 second
@23:56:45.77 AFV.select :holdingCoil
@23:56:45.87 ASV.dial :waste1
@23:56:46.27 ATV.dial :air3
@23:56:47.63 AS.reconfigure ASslow1
@23:56:47.69 AS.seek 0.51ml
@23:56:50.00 delay 2 seconds
@23:56:52.03 ATV.dial :bleach
@23:56:52.69 AS.seek 0.81ml
@23:57:53.26 delay 3 seconds
@23:57:56.29 ATV.dial :air3
@23:57:56.88 delay 3 seconds
@23:57:59.95 AS.seek 0.835ml
@23:58:05.21 delay 3 seconds
@23:58:08.24 ATV.dial :bleach
@23:58:08.86 AS.seek 0.82ml
@23:58:12.16 delay 3 seconds
@23:58:15.19 ARV.dial :waste
@23:58:15.83 delay 3 seconds
@23:58:18.85 ATV.dial :ARV
@23:58:19.79 AS.reconfigure ASconfig
@23:58:19.84 AS.seek 0.47ml
@23:58:40.24 AFV.select :carrierSolution
@23:58:40.36 delay 2 seconds
@23:58:42.38 AS.seek 1ml
@23:59:13.12 delay 1 second
@23:59:14.15 AFV.select :holdingCoil
@23:59:14.25 delay 1 second
@23:59:15.27 AS.reconfigure ASfast
@23:59:15.33 AS.seek 0ml
@23:59:42.66 AFV.select :carrierSolution
@23:59:42.78 delay 2 seconds
@23:59:44.80 AS.reconfigure ASconfig
@23:59:44.85 AS.seek 1ml
@00:00:42.58CEST20-Apr-21 delay 1 second
@00:00:43.61 AFV.select :holdingCoil
@00:00:43.71 delay 1 second
@00:00:44.73 AS.reconfigure ASfast
@00:00:44.78 AS.seek 0ml
@00:01:12.12 AFV.select :carrierSolution
@00:01:12.24 delay 2 seconds
@00:01:14.26 AS.reconfigure ASconfig
@00:01:14.31 AS.seek 1ml
@00:02:12.07 delay 1 second
@00:02:13.10 AFV.select :holdingCoil
@00:02:13.20 delay 1 second
@00:02:14.22 AS.reconfigure ASfast
@00:02:14.27 AS.seek 0ml
@00:02:41.60 ARV.dial between carrier and air1
@00:02:42.14 ATV.dial :air3
@00:02:43.50 AS.reconfigure ASslow1
@00:02:43.56 AS.seek 0.01ml
@00:02:45.88 delay 2 seconds
@00:02:47.91 ATV.dial :bleach
@00:02:48.57 AS.seek 0.81ml
@00:05:29.69 delay 3 seconds
@00:05:32.72 ATV.dial :air3
@00:05:33.33 delay 3 seconds
@00:05:36.39 AS.seek 0.835ml
@00:05:41.80 delay 3 seconds
@00:05:44.83 ATV.dial :bleach
@00:05:45.47 AS.seek 0.82ml
@00:05:48.73 delay 3 seconds
@00:05:51.76 ATV.dial PCR
@00:05:52.19 AS.reconfigure ASconfig
@00:05:52.66 AS.seek 0ml
@00:06:40.07 PCR.program :bleachProfile
@00:06:40.14 PCR program should complete in < 2:45
@00:06:40.15 PCR.run 10:00
@00:09:35.94 PCR.off
@00:09:35.98 AFV.select :carrierSolution
@00:09:36.09 delay 2 seconds
@00:09:38.11 AS.seek 1ml
@00:10:35.90 delay 1 second
@00:10:36.93 AFV.select :holdingCoil
@00:10:37.03 delay 1 second
@00:10:38.05 AS.reconfigure ASfast
@00:10:38.11 AS.seek 0ml
@00:11:05.46 delay 3 seconds
@00:11:08.49 AFV.select :carrierSolution
@00:11:08.59 delay 2 seconds
@00:11:10.61 AS.reconfigure ASconfig
@00:11:10.68 AS.seek 1ml
@00:12:08.46 delay 1 second
@00:12:09.49 AFV.select :holdingCoil
@00:12:09.59 delay 1 second
@00:12:10.61 AS.reconfigure ASfast
@00:12:10.67 AS.seek 0ml
@00:12:38.05 delay 3 seconds
@00:12:41.08 AFV.select :carrierSolution
@00:12:41.18 delay 2 seconds
@00:12:43.20 AS.reconfigure ASconfig
@00:12:43.25 AS.seek 1ml
@00:13:41.01 delay 1 second
@00:13:42.04 AFV.select :holdingCoil
@00:13:42.14 delay 1 second
@00:13:43.16 AS.reconfigure ASfast
@00:13:43.22 AS.seek 0ml
@00:14:10.55 delay 3 seconds
@00:14:13.58 AFV.select :carrierSolution
@00:14:13.68 delay 2 seconds
@00:14:15.70 AS.reconfigure ASconfig
@00:14:15.76 AS.seek 1ml
@00:15:13.52 delay 1 second
@00:15:14.55 AFV.select :holdingCoil
@00:15:14.65 delay 1 second
@00:15:15.67 AS.reconfigure ASfast
@00:15:15.73 AS.seek 0ml
@00:15:43.08 delay 3 seconds
@00:15:46.11 AFV.select :carrierSolution
@00:15:46.21 delay 2 seconds
@00:15:48.23 AS.reconfigure ASconfig
@00:15:48.29 AS.seek 1ml
@00:16:46.04 delay 1 second
@00:16:47.07 AFV.select :holdingCoil
@00:16:47.17 delay 1 second
@00:16:48.19 AS.reconfigure ASnorm
@00:16:48.24 AS.seek 0ml
@00:17:38.97 Setting up PCR for AARPP4 -- writing /var/log/esp/AARPP421apr2000h.pcr
@00:17:38.99 Building PCR Slug from ASV with MM2 mix, PP4 primer
@00:17:39.01 Priming with carrier
@00:17:39.03 ATV.dial ARV
@00:17:39.77 ARV.dial :carrier
@00:17:40.11 AS.seek 0.5ml
@00:18:05.66 AFV.select :carrierSolution
@00:18:05.79 delay 2 seconds
@00:18:07.81 AS.reconfigure ASconfig
@00:18:07.87 AS.seek 1ml
@00:18:36.88 delay 1 second
@00:18:37.91 AFV.select :holdingCoil
@00:18:38.01 delay 1 second
@00:18:39.03 ATV.dial :waste
@00:18:40.81 AS.reconfigure ASnorm
@00:18:40.87 AS.seek 0ml
@00:19:31.57 AFV.select :carrierSolution
@00:19:32.10 delay 2 seconds
@00:19:34.12 AS.reconfigure ASconfig
@00:19:34.18 AS.seek 0.5ml
@00:20:03.25 delay 1 second
@00:20:04.27 AFV.select :holdingCoil
@00:20:04.37 Building Slug
@00:20:04.38 ATV.dial :ARV
@00:20:06.14 delay 2 seconds
@00:20:08.16 ARV.dial :air1
@00:20:08.57 delay 2 seconds
@00:20:10.63 AS.reconfigure ASslow2
@00:20:10.69 AS.seek 0.506ml
@00:20:17.38 delay 2 seconds
@00:20:19.41 ARV.dial :MM2
@00:20:21.00 delay 2 seconds
@00:20:23.02 Adding MM2
@00:20:23.11 AS.seek 0.524ml
@00:20:41.47 delay 25 seconds
@00:21:06.49 ARV.dial :PP4
@00:21:06.91 Adding PP4
@00:21:06.92 delay 2 seconds
@00:21:09.00 AS.seek 0.53ml
@00:21:15.37 delay 10 seconds
@00:21:25.40 ARV.dial :air3
@00:21:25.83 adding ARV air
@00:21:25.84 delay 2 seconds
@00:21:27.92 AS.seek 0.536ml
@00:21:34.54 delay 5 seconds
@00:21:39.57 ARV.dial :carrier
@00:21:40.91 delay 7 seconds
@00:21:47.99 AS.seek 0.594ml
@00:22:46.51 delay 5 seconds
@00:22:51.54 ATV.dial :ASV
@00:22:51.97 delay 2 seconds
@00:22:54.04 AS.seek 0.6ml
@00:23:00.32 delay 4 seconds
@00:23:04.35 ATV.dial ARV
@00:23:04.92 delay 2 seconds
@00:23:06.94 ARV.dial :carrier
@00:23:06.95 delay 2 seconds
@00:23:09.03 AS.seek 0.65ml
@00:23:59.66 delay 4 seconds
@00:24:03.69 ATV.dial PCR
@00:24:04.33 ASV.dial :waste1
@00:24:04.35 ARV.dial :air1
@00:24:04.78 Positioning Slug by 0.324ml
@00:24:04.86 AS.seek 0.326ml
@00:29:31.04 delay 5 seconds
@00:29:36.07 ATV.dial ARV
@00:29:36.87 ASV.dial :PCRlock
@00:29:37.28 delay 5 seconds
@00:29:42.30 Running AARPP4 Profile58CIQ2 thermocycling
@00:29:42.31 <PCR> PCR.program Profile58CIQ2
@00:29:42.43 Profile58CIQ2 should complete in < 1:36:15
@00:29:42.44 PCR.run 2:05:18.75
@01:50:57.38 PCR.off
@01:50:57.94 <MFBPCR> Running AARPP4 MeltProfile58 thermocycling
@01:50:57.96 <PCR> PCR.program MeltProfile58
@01:50:58.06 MeltProfile58 should complete in < 12:33
@01:50:58.07 PCR.run 20:41.25
@01:58:48.94 PCR.off
@01:58:49.91 <MFBPCR> PCR for AARPP4 completed -- closed /var/log/esp/AARPP421apr2000h.pcr
@01:58:49.98 Cleaning PCR
@01:58:50.00 AFV.select :carrierSolution
@01:58:50.12 delay 2 seconds
@01:58:52.14 AS.reconfigure ASconfig
@01:58:52.20 AS.seek 0.5ml
@01:59:02.49 delay 1 second
@01:59:03.52 AFV.select :holdingCoil
@01:59:03.62 ASV.dial :waste1
@01:59:04.02 ATV.dial :air3
@01:59:05.38 AS.reconfigure ASslow1
@01:59:05.43 AS.seek 0.51ml
@01:59:07.70 delay 2 seconds
@01:59:09.73 ATV.dial :bleach
@01:59:10.38 AS.seek 0.81ml
@02:00:10.99 delay 3 seconds
@02:00:14.02 ATV.dial :air3
@02:00:14.61 delay 3 seconds
@02:00:17.67 AS.seek 0.835ml
@02:00:22.95 delay 3 seconds
@02:00:25.98 ATV.dial :bleach
@02:00:26.61 AS.seek 0.82ml
@02:00:30.05 delay 3 seconds
@02:00:33.42 ARV.dial :waste
@02:00:34.11 delay 3 seconds
@02:00:37.13 ATV.dial :ARV
@02:00:38.06 AS.reconfigure ASconfig
@02:00:38.12 AS.seek 0.47ml
@02:00:58.52 AFV.select :carrierSolution
@02:00:58.64 delay 2 seconds
@02:01:00.66 AS.seek 1ml
@02:01:31.41 delay 1 second
@02:01:32.43 AFV.select :holdingCoil
@02:01:32.53 delay 1 second
@02:01:33.55 AS.reconfigure ASfast
@02:01:33.61 AS.seek 0ml
@02:02:00.93 AFV.select :carrierSolution
@02:02:01.05 delay 2 seconds
@02:02:03.07 AS.reconfigure ASconfig
@02:02:03.13 AS.seek 1ml
@02:03:00.86 delay 1 second
@02:03:01.89 AFV.select :holdingCoil
@02:03:01.99 delay 1 second
@02:03:03.01 AS.reconfigure ASfast
@02:03:03.07 AS.seek 0ml
@02:03:30.41 AFV.select :carrierSolution
@02:03:30.52 delay 2 seconds
@02:03:32.54 AS.reconfigure ASconfig
@02:03:32.60 AS.seek 1ml
@02:04:30.33 delay 1 second
@02:04:31.36 AFV.select :holdingCoil
@02:04:31.46 delay 1 second
@02:04:32.48 AS.reconfigure ASfast
@02:04:32.54 AS.seek 0ml
@02:04:59.86 ARV.dial between carrier and air1
@02:05:00.41 ATV.dial :air3
@02:05:01.77 AS.reconfigure ASslow1
@02:05:01.83 AS.seek 0.01ml
@02:05:04.30 delay 2 seconds
@02:05:06.33 ATV.dial :bleach
@02:05:06.97 AS.seek 0.81ml
@02:07:48.11 delay 3 seconds
@02:07:51.14 ATV.dial :air3
@02:07:51.74 delay 3 seconds
@02:07:54.80 AS.seek 0.835ml
@02:08:00.08 delay 3 seconds
@02:08:03.11 ATV.dial :bleach
@02:08:03.75 AS.seek 0.82ml
@02:08:07.18 delay 3 seconds
@02:08:10.21 ATV.dial PCR
@02:08:10.64 AS.reconfigure ASconfig
@02:08:10.69 AS.seek 0ml
@02:08:58.08 PCR.program :bleachProfile
@02:08:58.15 PCR program should complete in < 2:45
@02:08:58.16 PCR.run 10:00
@02:11:53.96 PCR.off
@02:11:54.01 AFV.select :carrierSolution
@02:11:54.12 delay 2 seconds
@02:11:56.14 AS.seek 1ml
@02:12:53.91 delay 1 second
@02:12:54.94 AFV.select :holdingCoil
@02:12:55.04 delay 1 second
@02:12:56.06 AS.reconfigure ASfast
@02:12:56.11 AS.seek 0ml
@02:13:23.44 delay 3 seconds
@02:13:26.86 AFV.select :carrierSolution
@02:13:26.99 delay 2 seconds
@02:13:29.01 AS.reconfigure ASconfig
@02:13:29.07 AS.seek 1ml
@02:14:26.86 delay 1 second
@02:14:27.89 AFV.select :holdingCoil
@02:14:27.99 delay 1 second
@02:14:29.01 AS.reconfigure ASfast
@02:14:29.07 AS.seek 0ml
@02:14:56.42 delay 3 seconds
@02:14:59.44 AFV.select :carrierSolution
@02:14:59.54 delay 2 seconds
@02:15:01.56 AS.reconfigure ASconfig
@02:15:01.63 AS.seek 1ml
@02:15:59.41 delay 1 second
@02:16:00.44 AFV.select :holdingCoil
@02:16:00.54 delay 1 second
@02:16:01.56 AS.reconfigure ASfast
@02:16:01.62 AS.seek 0ml
@02:16:28.98 delay 3 seconds
@02:16:32.00 AFV.select :carrierSolution
@02:16:32.10 delay 2 seconds
@02:16:34.12 AS.reconfigure ASconfig
@02:16:34.18 AS.seek 1ml
@02:17:31.96 delay 1 second
@02:17:32.99 AFV.select :holdingCoil
@02:17:33.09 delay 1 second
@02:17:34.11 AS.reconfigure ASfast
@02:17:34.17 AS.seek 0ml
@02:18:01.49 delay 3 seconds
@02:18:04.52 AFV.select :carrierSolution
@02:18:04.62 delay 2 seconds
@02:18:06.64 AS.reconfigure ASconfig
@02:18:06.71 AS.seek 1ml
@02:19:04.46 delay 1 second
@02:19:05.49 AFV.select :holdingCoil
@02:19:05.59 delay 1 second
@02:19:06.61 AS.reconfigure ASnorm
@02:19:06.66 AS.seek 0ml
@02:19:57.39 Setting up PCR for AALPP3 -- writing /var/log/esp/AALPP321apr2002h.pcr
@02:19:57.41 Building PCR Slug from ASV with MM2 mix, PP3 primer
@02:19:57.43 Priming with carrier
@02:19:57.45 ATV.dial ARV
@02:19:58.19 ARV.dial :carrier
@02:19:58.53 AS.seek 0.5ml
@02:20:24.07 AFV.select :carrierSolution
@02:20:24.20 delay 2 seconds
@02:20:26.22 AS.reconfigure ASconfig
@02:20:26.28 AS.seek 1ml
@02:20:55.31 delay 1 second
@02:20:56.33 AFV.select :holdingCoil
@02:20:56.43 delay 1 second
@02:20:57.45 ATV.dial :waste
@02:20:59.25 AS.reconfigure ASnorm
@02:20:59.31 AS.seek 0ml
@02:21:49.99 AFV.select :carrierSolution
@02:21:50.11 delay 2 seconds
@02:21:52.13 AS.reconfigure ASconfig
@02:21:52.19 AS.seek 0.5ml
@02:22:21.21 delay 1 second
@02:22:22.24 AFV.select :holdingCoil
@02:22:22.34 Building Slug
@02:22:22.35 ATV.dial :ARV
@02:22:24.11 delay 2 seconds
@02:22:26.13 ARV.dial :air1
@02:22:26.54 delay 2 seconds
@02:22:28.60 AS.reconfigure ASslow2
@02:22:28.67 AS.seek 0.506ml
@02:22:34.86 delay 2 seconds
@02:22:36.89 ARV.dial :MM2
@02:22:38.48 delay 2 seconds
@02:22:40.50 Adding MM2
@02:22:40.58 AS.seek 0.524ml
@02:22:58.80 delay 25 seconds
@02:23:23.83 ARV.dial :PP3
@02:23:24.23 Adding PP3
@02:23:24.24 delay 2 seconds
@02:23:26.32 AS.seek 0.53ml
@02:23:32.55 delay 10 seconds
@02:23:42.59 ARV.dial :air2
@02:23:43.01 adding ARV air
@02:23:43.02 delay 2 seconds
@02:23:45.10 AS.seek 0.536ml
@02:23:51.50 delay 5 seconds
@02:23:56.53 ARV.dial :carrier
@02:23:57.91 delay 7 seconds
@02:24:04.98 AS.seek 0.594ml
@02:25:03.64 delay 5 seconds
@02:25:08.67 ATV.dial :ASV
@02:25:09.11 delay 2 seconds
@02:25:11.18 AS.seek 0.6ml
@02:25:17.58 delay 4 seconds
@02:25:21.61 ATV.dial ARV
@02:25:22.19 delay 2 seconds
@02:25:24.21 ARV.dial :carrier
@02:25:24.22 delay 2 seconds
@02:25:26.30 AS.seek 0.65ml
@02:26:17.41 delay 4 seconds
@02:26:21.44 ATV.dial PCR
@02:26:22.08 ASV.dial :waste1
@02:26:22.10 ARV.dial :air1
@02:26:22.54 Positioning Slug by 0.324ml
@02:26:22.62 AS.seek 0.326ml
@02:31:48.96 delay 5 seconds
@02:31:53.99 ATV.dial ARV
@02:31:54.80 ASV.dial :PCRlock
@02:31:55.22 delay 5 seconds
@02:32:00.24 Running AALPP3 Profile58CIQ2 thermocycling
@02:32:00.25 <PCR> PCR.program Profile58CIQ2
@02:32:00.37 Profile58CIQ2 should complete in < 1:36:15
@02:32:00.38 PCR.run 2:05:18.75
@03:53:09.69 PCR.off
@03:53:10.25 <MFBPCR> Running AALPP3 MeltProfile58 thermocycling
@03:53:10.26 <PCR> PCR.program MeltProfile58
@03:53:10.37 MeltProfile58 should complete in < 12:33
@03:53:10.38 PCR.run 20:41.25
@04:01:01.20 PCR.off
@04:01:02.17 <MFBPCR> PCR for AALPP3 completed -- closed /var/log/esp/AALPP321apr2002h.pcr
@04:01:02.24 Cleaning PCR
@04:01:02.25 AFV.select :carrierSolution
@04:01:02.37 delay 2 seconds
@04:01:04.39 AS.reconfigure ASconfig
@04:01:04.45 AS.seek 0.5ml
@04:01:14.75 delay 1 second
@04:01:15.78 AFV.select :holdingCoil
@04:01:15.88 ASV.dial :waste1
@04:01:16.27 ATV.dial :air3
@04:01:17.63 AS.reconfigure ASslow1
@04:01:17.69 AS.seek 0.51ml
@04:01:19.98 delay 2 seconds
@04:01:22.00 ATV.dial :bleach
@04:01:22.64 AS.seek 0.81ml
@04:02:23.21 delay 3 seconds
@04:02:26.24 ATV.dial :air3
@04:02:26.83 delay 3 seconds
@04:02:29.89 AS.seek 0.835ml
@04:02:35.16 delay 3 seconds
@04:02:38.19 ATV.dial :bleach
@04:02:38.83 AS.seek 0.82ml
@04:02:42.18 delay 3 seconds
@04:02:45.21 ARV.dial :waste
@04:02:45.86 delay 3 seconds
@04:02:48.88 ATV.dial :ARV
@04:02:49.81 AS.reconfigure ASconfig
@04:02:49.86 AS.seek 0.47ml
@04:03:10.25 AFV.select :carrierSolution
@04:03:10.37 delay 2 seconds
@04:03:12.39 AS.seek 1ml
@04:03:43.13 delay 1 second
@04:03:44.16 AFV.select :holdingCoil
@04:03:44.26 delay 1 second
@04:03:45.28 AS.reconfigure ASfast
@04:03:45.33 AS.seek 0ml
@04:04:12.68 AFV.select :carrierSolution
@04:04:12.80 delay 2 seconds
@04:04:14.82 AS.reconfigure ASconfig
@04:04:14.87 AS.seek 1ml
@04:05:12.61 delay 1 second
@04:05:13.64 AFV.select :holdingCoil
@04:05:13.74 delay 1 second
@04:05:14.76 AS.reconfigure ASfast
@04:05:14.81 AS.seek 0ml
@04:05:42.14 AFV.select :carrierSolution
@04:05:42.26 delay 2 seconds
@04:05:44.28 AS.reconfigure ASconfig
@04:05:44.34 AS.seek 1ml
@04:06:42.13 delay 1 second
@04:06:43.16 AFV.select :holdingCoil
@04:06:43.26 delay 1 second
@04:06:44.28 AS.reconfigure ASfast
@04:06:44.33 AS.seek 0ml
@04:07:11.69 ARV.dial between carrier and air1
@04:07:12.23 ATV.dial :air3
@04:07:13.59 AS.reconfigure ASslow1
@04:07:13.65 AS.seek 0.01ml
@04:07:16.11 delay 2 seconds
@04:07:18.14 ATV.dial :bleach
@04:07:18.80 AS.seek 0.81ml
@04:09:59.97 delay 3 seconds
@04:10:02.99 ATV.dial :air3
@04:10:03.58 delay 3 seconds
@04:10:06.64 AS.seek 0.835ml
@04:10:11.95 delay 3 seconds
@04:10:14.97 ATV.dial :bleach
@04:10:15.61 AS.seek 0.82ml
@04:10:18.89 delay 3 seconds
@04:10:21.92 ATV.dial PCR
@04:10:22.34 AS.reconfigure ASconfig
@04:10:22.39 AS.seek 0ml
@04:11:09.82 PCR.program :bleachProfile
@04:11:09.89 PCR program should complete in < 2:45
@04:11:09.91 PCR.run 10:00
@04:14:06.12 PCR.off
@04:14:06.16 AFV.select :carrierSolution
@04:14:06.27 delay 2 seconds
@04:14:08.29 AS.seek 1ml
@04:15:06.08 delay 1 second
@04:15:07.11 AFV.select :holdingCoil
@04:15:07.21 delay 1 second
@04:15:08.23 AS.reconfigure ASfast
@04:15:08.29 AS.seek 0ml
@04:15:35.64 delay 3 seconds
@04:15:38.66 AFV.select :carrierSolution
@04:15:38.76 delay 2 seconds
@04:15:40.78 AS.reconfigure ASconfig
@04:15:40.86 AS.seek 1ml
@04:16:38.64 delay 1 second
@04:16:39.67 AFV.select :holdingCoil
@04:16:39.77 delay 1 second
@04:16:40.79 AS.reconfigure ASfast
@04:16:40.85 AS.seek 0ml
@04:17:08.19 delay 3 seconds
@04:17:11.22 AFV.select :carrierSolution
@04:17:11.32 delay 2 seconds
@04:17:13.34 AS.reconfigure ASconfig
@04:17:13.41 AS.seek 1ml
@04:18:11.14 delay 1 second
@04:18:12.17 AFV.select :holdingCoil
@04:18:12.27 delay 1 second
@04:18:13.29 AS.reconfigure ASfast
@04:18:13.34 AS.seek 0ml
@04:18:40.70 delay 3 seconds
@04:18:43.72 AFV.select :carrierSolution
@04:18:43.82 delay 2 seconds
@04:18:45.84 AS.reconfigure ASconfig
@04:18:45.91 AS.seek 1ml
@04:19:43.67 delay 1 second
@04:19:44.70 AFV.select :holdingCoil
@04:19:44.80 delay 1 second
@04:19:45.82 AS.reconfigure ASfast
@04:19:45.88 AS.seek 0ml
@04:20:13.21 delay 3 seconds
@04:20:16.24 AFV.select :carrierSolution
@04:20:16.34 delay 2 seconds
@04:20:18.36 AS.reconfigure ASconfig
@04:20:18.42 AS.seek 1ml
@04:21:16.21 delay 1 second
@04:21:17.24 AFV.select :holdingCoil
@04:21:17.34 delay 1 second
@04:21:18.36 AS.reconfigure ASnorm
@04:21:18.41 AS.seek 0ml
@04:22:09.17 Setting up PCR for ACOPP5 -- writing /var/log/esp/ACOPP521apr2004h.pcr
@04:22:09.19 Building PCR Slug from ASV with MM3 mix, PP5 primer
@04:22:09.21 Priming with carrier
@04:22:09.23 ATV.dial ARV
@04:22:09.88 ARV.dial :carrier
@04:22:10.24 AS.seek 0.5ml
@04:22:35.77 AFV.select :carrierSolution
@04:22:35.90 delay 2 seconds
@04:22:37.92 AS.reconfigure ASconfig
@04:22:37.98 AS.seek 1ml
@04:23:06.99 delay 1 second
@04:23:08.02 AFV.select :holdingCoil
@04:23:08.12 delay 1 second
@04:23:09.14 ATV.dial :waste
@04:23:10.92 AS.reconfigure ASnorm
@04:23:10.98 AS.seek 0ml
@04:24:01.67 AFV.select :carrierSolution
@04:24:01.79 delay 2 seconds
@04:24:03.81 AS.reconfigure ASconfig
@04:24:03.87 AS.seek 0.5ml
@04:24:32.94 delay 1 second
@04:24:33.97 AFV.select :holdingCoil
@04:24:34.07 Building Slug
@04:24:34.08 ATV.dial :ARV
@04:24:35.84 delay 2 seconds
@04:24:37.86 ARV.dial :air1
@04:24:38.26 delay 2 seconds
@04:24:40.32 AS.reconfigure ASslow2
@04:24:40.39 AS.seek 0.506ml
@04:24:46.67 delay 2 seconds
@04:24:48.70 ARV.dial :MM3
@04:24:49.81 delay 2 seconds
@04:24:51.83 Adding MM3
@04:24:51.91 AS.seek 0.524ml
@04:25:10.28 delay 25 seconds
@04:25:35.31 ARV.dial :PP5
@04:25:35.73 Adding PP5
@04:25:35.74 delay 2 seconds
@04:25:37.82 AS.seek 0.53ml
@04:25:44.32 delay 10 seconds
@04:25:54.36 ARV.dial :air3
@04:25:55.17 adding ARV air
@04:25:55.18 delay 2 seconds
@04:25:57.26 AS.seek 0.536ml
@04:26:03.72 delay 5 seconds
@04:26:08.74 ARV.dial :carrier
@04:26:10.08 delay 7 seconds
@04:26:17.16 AS.seek 0.594ml
@04:27:15.92 delay 5 seconds
@04:27:20.94 ATV.dial :ASV
@04:27:21.37 delay 2 seconds
@04:27:23.44 AS.seek 0.6ml
@04:27:29.82 delay 4 seconds
@04:27:33.85 ATV.dial ARV
@04:27:34.43 delay 2 seconds
@04:27:36.45 ARV.dial :carrier
@04:27:36.46 delay 2 seconds
@04:27:38.54 AS.seek 0.65ml
@04:28:29.33 delay 4 seconds
@04:28:33.36 ATV.dial PCR
@04:28:34.00 ASV.dial :waste1
@04:28:34.02 ARV.dial :air1
@04:28:34.46 Positioning Slug by 0.324ml
@04:28:34.54 AS.seek 0.326ml
@04:34:00.89 delay 5 seconds
@04:34:05.92 ATV.dial ARV
@04:34:06.72 ASV.dial :PCRlock
@04:34:07.14 delay 5 seconds
@04:34:12.16 Running ACOPP5 ProfileIPc thermocycling
@04:34:12.17 <PCR> PCR.program ProfileIPc
@04:34:12.29 ProfileIPc should complete in < 1:26:35
@04:34:12.30 PCR.run 1:53:13.75
@05:43:07.43 PCR.off
@05:43:07.99 <MFBPCR> PCR for ACOPP5 completed -- closed /var/log/esp/ACOPP521apr2004h.pcr
@05:43:08.06 Cleaning PCR
@05:43:08.08 AFV.select :carrierSolution
@05:43:08.20 delay 2 seconds
@05:43:10.22 AS.reconfigure ASconfig
@05:43:10.27 AS.seek 0.5ml
@05:43:20.58 delay 1 second
@05:43:21.61 AFV.select :holdingCoil
@05:43:21.71 ASV.dial :waste1
@05:43:22.11 ATV.dial :air3
@05:43:23.45 AS.reconfigure ASslow1
@05:43:23.51 AS.seek 0.51ml
@05:43:25.79 delay 2 seconds
@05:43:27.82 ATV.dial :bleach
@05:43:28.47 AS.seek 0.81ml
@05:44:29.16 delay 3 seconds
@05:44:32.19 ATV.dial :air3
@05:44:32.78 delay 3 seconds
@05:44:35.84 AS.seek 0.835ml
@05:44:41.13 delay 3 seconds
@05:44:44.16 ATV.dial :bleach
@05:44:44.80 AS.seek 0.82ml
@05:44:48.11 delay 3 seconds
@05:44:51.14 ARV.dial :waste
@05:44:51.78 delay 3 seconds
@05:44:54.80 ATV.dial :ARV
@05:44:55.73 AS.reconfigure ASconfig
@05:44:55.78 AS.seek 0.47ml
@05:45:16.19 AFV.select :carrierSolution
@05:45:16.31 delay 2 seconds
@05:45:18.33 AS.seek 1ml
@05:45:49.08 delay 1 second
@05:45:50.11 AFV.select :holdingCoil
@05:45:50.21 delay 1 second
@05:45:51.23 AS.reconfigure ASfast
@05:45:51.28 AS.seek 0ml
@05:46:18.61 AFV.select :carrierSolution
@05:46:18.73 delay 2 seconds
@05:46:20.75 AS.reconfigure ASconfig
@05:46:20.80 AS.seek 1ml
@05:47:18.55 delay 1 second
@05:47:19.58 AFV.select :holdingCoil
@05:47:19.68 delay 1 second
@05:47:20.70 AS.reconfigure ASfast
@05:47:20.76 AS.seek 0ml
@05:47:48.10 AFV.select :carrierSolution
@05:47:48.22 delay 2 seconds
@05:47:50.24 AS.reconfigure ASconfig
@05:47:50.29 AS.seek 1ml
@05:48:48.03 delay 1 second
@05:48:49.06 AFV.select :holdingCoil
@05:48:49.16 delay 1 second
@05:48:50.18 AS.reconfigure ASfast
@05:48:50.23 AS.seek 0ml
@05:49:17.56 ARV.dial between carrier and air1
@05:49:18.09 ATV.dial :air3
@05:49:19.45 AS.reconfigure ASslow1
@05:49:19.51 AS.seek 0.01ml
@05:49:21.99 delay 2 seconds
@05:49:24.02 ATV.dial :bleach
@05:49:24.66 AS.seek 0.81ml
@05:52:05.81 delay 3 seconds
@05:52:08.84 ATV.dial :air3
@05:52:09.43 delay 3 seconds
@05:52:12.49 AS.seek 0.835ml
@05:52:17.89 delay 3 seconds
@05:52:20.92 ATV.dial :bleach
@05:52:21.56 AS.seek 0.82ml
@05:52:24.85 delay 3 seconds
@05:52:27.88 ATV.dial PCR
@05:52:28.31 AS.reconfigure ASconfig
@05:52:28.36 AS.seek 0ml
@05:53:15.77 PCR.program :bleachProfile
@05:53:15.84 PCR program should complete in < 2:45
@05:53:15.85 PCR.run 10:00
@05:56:12.07 PCR.off
@05:56:12.11 AFV.select :carrierSolution
@05:56:12.21 delay 2 seconds
@05:56:14.23 AS.seek 1ml
@05:57:11.99 delay 1 second
@05:57:13.02 AFV.select :holdingCoil
@05:57:13.12 delay 1 second
@05:57:14.14 AS.reconfigure ASfast
@05:57:14.20 AS.seek 0ml
@05:57:41.55 delay 3 seconds
@05:57:44.58 AFV.select :carrierSolution
@05:57:44.68 delay 2 seconds
@05:57:46.70 AS.reconfigure ASconfig
@05:57:46.76 AS.seek 1ml
@05:58:44.52 delay 1 second
@05:58:45.55 AFV.select :holdingCoil
@05:58:45.65 delay 1 second
@05:58:46.67 AS.reconfigure ASfast
@05:58:46.73 AS.seek 0ml
@05:59:14.07 delay 3 seconds
@05:59:17.09 AFV.select :carrierSolution
@05:59:17.19 delay 2 seconds
@05:59:19.21 AS.reconfigure ASconfig
@05:59:19.26 AS.seek 1ml
@06:00:17.05 delay 1 second
@06:00:18.08 AFV.select :holdingCoil
@06:00:18.18 delay 1 second
@06:00:19.20 AS.reconfigure ASfast
@06:00:19.25 AS.seek 0ml
@06:00:46.59 delay 3 seconds
@06:00:49.61 AFV.select :carrierSolution
@06:00:49.71 delay 2 seconds
@06:00:51.73 AS.reconfigure ASconfig
@06:00:51.80 AS.seek 1ml
@06:01:49.58 delay 1 second
@06:01:50.61 AFV.select :holdingCoil
@06:01:50.71 delay 1 second
@06:01:51.73 AS.reconfigure ASfast
@06:01:51.78 AS.seek 0ml
@06:02:19.11 delay 3 seconds
@06:02:22.14 AFV.select :carrierSolution
@06:02:22.24 delay 2 seconds
@06:02:24.26 AS.reconfigure ASconfig
@06:02:24.33 AS.seek 1ml
@06:03:22.11 delay 1 second
@06:03:23.14 AFV.select :holdingCoil
@06:03:23.24 delay 1 second
@06:03:24.26 AS.reconfigure ASnorm
@06:03:24.31 AS.seek 0ml
@06:04:15.02 Setting up PCR for AIPc -- writing /var/log/esp/AIPc21apr2006h.pcr
@06:04:15.04 Building PCR Slug from ASV with MM3 mix, PP6 primer
@06:04:15.06 Priming with carrier
@06:04:15.08 ATV.dial ARV
@06:04:15.72 ARV.dial :carrier
@06:04:16.06 AS.seek 0.5ml
@06:04:41.55 AFV.select :carrierSolution
@06:04:41.67 delay 2 seconds
@06:04:43.69 AS.reconfigure ASconfig
@06:04:43.75 AS.seek 1ml
@06:05:12.77 delay 1 second
@06:05:13.80 AFV.select :holdingCoil
@06:05:13.90 delay 1 second
@06:05:14.92 ATV.dial :waste
@06:05:16.70 AS.reconfigure ASnorm
@06:05:16.76 AS.seek 0ml
@06:06:07.44 AFV.select :carrierSolution
@06:06:07.56 delay 2 seconds
@06:06:09.58 AS.reconfigure ASconfig
@06:06:09.64 AS.seek 0.5ml
@06:06:38.69 delay 1 second
@06:06:39.72 AFV.select :holdingCoil
@06:06:39.82 Building Slug
@06:06:39.83 ATV.dial :ARV
@06:06:41.59 delay 2 seconds
@06:06:43.61 ARV.dial :air1
@06:06:44.42 delay 2 seconds
@06:06:46.49 AS.reconfigure ASslow2
@06:06:46.56 AS.seek 0.506ml
@06:06:52.76 delay 2 seconds
@06:06:54.78 ARV.dial :MM3
@06:06:55.89 delay 2 seconds
@06:06:57.91 Adding MM3
@06:06:58.00 AS.seek 0.524ml
@06:07:16.39 delay 25 seconds
@06:07:41.41 ARV.dial :PP6
@06:07:41.81 Adding PP6
@06:07:41.82 delay 2 seconds
@06:07:43.90 AS.seek 0.53ml
@06:07:50.76 delay 10 seconds
@06:08:00.79 ARV.dial :air1
@06:08:01.67 adding ARV air
@06:08:01.68 delay 2 seconds
@06:08:03.76 AS.seek 0.536ml
@06:08:10.35 delay 5 seconds
@06:08:15.38 ARV.dial :carrier
@06:08:15.81 delay 7 seconds
@06:08:22.89 AS.seek 0.594ml
@06:09:21.57 delay 5 seconds
@06:09:26.59 ATV.dial :ASV
@06:09:27.01 delay 2 seconds
@06:09:29.08 AS.seek 0.6ml
@06:09:35.55 delay 4 seconds
@06:09:39.58 ATV.dial ARV
@06:09:40.15 delay 2 seconds
@06:09:42.17 ARV.dial :carrier
@06:09:42.18 delay 2 seconds
@06:09:44.26 AS.seek 0.65ml
@06:10:35.11 delay 4 seconds
@06:10:39.14 ATV.dial PCR
@06:10:39.78 ASV.dial :waste1
@06:10:39.80 ARV.dial :air1
@06:10:40.23 Positioning Slug by 0.324ml
@06:10:40.32 AS.seek 0.326ml
@06:16:06.58 delay 5 seconds
@06:16:11.61 ATV.dial ARV
@06:16:12.42 ASV.dial :PCRlock
@06:16:12.82 delay 5 seconds
@06:16:17.84 Running AIPc ProfileIPc thermocycling
@06:16:17.85 <PCR> PCR.program ProfileIPc
@06:16:17.97 ProfileIPc should complete in < 1:26:35
@06:16:17.98 PCR.run 1:53:13.75
@07:25:20.47 PCR.off
@07:25:21.02 <MFBPCR> PCR for AIPc completed -- closed /var/log/esp/AIPc21apr2006h.pcr
@07:25:21.04 Full MFB Decontamination
@07:25:21.06 Decon extraction column and decon extSrc
@07:25:21.08 SPE.hold 2:46:40,55C
@07:25:21.14 ASV.dial :SPEdelivery
@07:25:21.82 delay 20 seconds
@07:25:41.85 AFV.select :carrierSolution
@07:25:41.95 delay 2 seconds
@07:25:43.97 AS.reconfigure ASconfig
@07:25:44.02 AS.seek 0.5ml
@07:25:54.36 delay 1 second
@07:25:55.39 AFV.select :holdingCoil
@07:25:55.49 ATV.dial :air1
@07:25:57.73 AS.reconfigure ASslow1
@07:25:57.79 AS.seek 0.52ml
@07:26:02.14 ATV.dial :ASV
@07:26:03.91 ASV.dial :SPErecovery
@07:26:04.61 AS.seek 0.62ml
@07:26:25.02 ATV.dial :waste
@07:26:27.23 AS.reconfigure ASnorm
@07:26:27.29 AS.seek 0ml
@07:26:58.82 deconEluateRecoveryLine extSrc
@07:26:58.83 ATV.dial :waste
@07:26:58.86 AS.seek 0ml
@07:26:59.14 ATV.dial :air3
@07:26:59.91 AS.reconfigure ASslow1
@07:26:59.97 AS.seek 0.01ml
@07:27:02.27 delay 2 seconds
@07:27:04.30 ATV.dial :bleach
@07:27:04.80 AS.seek 0.31ml
@07:28:05.40 delay 3 seconds
@07:28:08.42 ATV.dial :air3
@07:28:09.06 delay 3 seconds
@07:28:12.13 AS.seek 0.335ml
@07:28:17.37 delay 3 seconds
@07:28:20.40 ATV.dial :bleach
@07:28:21.08 AS.seek 0.32ml
@07:28:24.42 delay 3 seconds
@07:28:27.45 ATV.dial :extSrc
@07:28:28.14 AS.seek 0ml
@07:29:32.77 AFV.select :carrierSolution
@07:29:32.89 delay 2 seconds
@07:29:34.91 AS.reconfigure ASconfig
@07:29:34.97 AS.seek 0.5ml
@07:30:04.00 delay 1 second
@07:30:05.03 AFV.select :holdingCoil
@07:30:05.13 ATV.dial :extSrc
@07:30:05.15 AS.reconfigure ASslow1
@07:30:05.21 AS.seek 0ml
@07:31:46.05 AFV.select :carrierSolution
@07:31:46.17 delay 2 seconds
@07:31:48.19 AS.reconfigure ASconfig
@07:31:48.25 AS.seek 0.5ml
@07:32:17.28 delay 1 second
@07:32:18.31 AFV.select :holdingCoil
@07:32:18.41 ATV.dial :extSrc
@07:32:18.43 AS.reconfigure ASslow1
@07:32:18.49 AS.seek 0ml
@07:33:59.30 AFV.select :carrierSolution
@07:33:59.42 delay 2 seconds
@07:34:01.44 AS.reconfigure ASconfig
@07:34:01.91 AS.seek 0.5ml
@07:34:30.94 delay 1 second
@07:34:31.97 AFV.select :holdingCoil
@07:34:32.07 ATV.dial :extSrc
@07:34:32.09 AS.reconfigure ASslow1
@07:34:32.15 AS.seek 0ml
@07:36:12.97 AFV.select :carrierSolution
@07:36:13.09 delay 2 seconds
@07:36:15.11 AS.reconfigure ASconfig
@07:36:15.17 AS.seek 0.5ml
@07:36:44.20 delay 1 second
@07:36:45.23 AFV.select :holdingCoil
@07:36:45.33 ATV.dial :extSrc
@07:36:45.35 AS.reconfigure ASslow1
@07:36:45.41 AS.seek 0ml
@07:38:26.24 ATV.dial :air2
@07:38:27.64 AS.reconfigure ASslow0
@07:38:27.70 AS.seek 0.5ml
@07:39:18.33 ATV.dial :extSrc
@07:39:19.90 AS.seek 0.02ml
@07:40:08.51 delay 1 second
@07:40:09.53 ATV.dial :waste
@07:40:10.90 AS.reconfigure ASconfig
@07:40:10.95 AS.seek 0ml
@07:40:12.39 AFV.select :holdingCoil
@07:40:12.41 ATV.dial :waste
@07:40:12.44 AS.seek 0ml
@07:40:12.52 AFV.select :carrierSolution
@07:40:12.62 delay 2 seconds
@07:40:14.64 AS.seek 0.5ml
@07:40:43.67 delay 1 second
@07:40:44.70 AFV.select :holdingCoil
@07:40:44.82 ATV.dial :air3
@07:40:45.72 AS.reconfigure ASslow1
@07:40:45.78 AS.seek 0.52ml
@07:40:50.22 ATV.dial :air3
@07:40:50.31 AS.seek 0.53ml
@07:40:52.70 delay 2 seconds
@07:40:54.73 ATV.dial :bleach
@07:40:55.23 AS.seek 0.71ml
@07:41:31.69 delay 3 seconds
@07:41:34.72 ATV.dial :air3
@07:41:35.31 delay 3 seconds
@07:41:38.38 AS.seek 0.735ml
@07:41:43.65 delay 3 seconds
@07:41:46.68 ATV.dial :bleach
@07:41:47.30 AS.seek 0.72ml
@07:41:50.59 delay 3 seconds
@07:41:53.62 ATV.dial ASV
@07:41:54.72 ASV.dial :SPErecovery
@07:41:54.81 AS.seek 0.365ml
@07:43:06.47 ATV.dial :waste
@07:43:08.50 AS.reconfigure ASnorm
@07:43:08.56 AS.seek 0ml
@07:43:27.28 ATV.dial :air3
@07:43:28.17 AS.reconfigure ASslow1
@07:43:28.23 AS.seek 0.01ml
@07:43:30.55 delay 2 seconds
@07:43:32.58 ATV.dial :bleach
@07:43:33.06 AS.seek 0.58ml
@07:45:27.95 delay 3 seconds
@07:45:30.98 ATV.dial :air3
@07:45:31.57 delay 3 seconds
@07:45:34.64 AS.seek 0.605ml
@07:45:40.01 delay 3 seconds
@07:45:43.04 ATV.dial :bleach
@07:45:43.69 AS.seek 0.59ml
@07:45:46.97 delay 3 seconds
@07:45:50.00 ATV.dial :ASV
@07:45:51.09 ASV.dial :wasteR
@07:45:51.50 AS.seek 0ml
@07:47:50.43 delay 1:00
@07:48:50.46 ATV.dial ASV
@07:48:50.47 AFV.select :carrierSolution
@07:48:50.59 delay 2 seconds
@07:48:52.61 AS.reconfigure ASconfig
@07:48:52.66 AS.seek 1ml
@07:49:50.41 delay 1 second
@07:49:51.44 AFV.select :holdingCoil
@07:49:51.54 delay 1 second
@07:49:52.56 ASV.dial :wasteR
@07:49:52.57 AS.reconfigure ASslow1
@07:49:52.64 AS.seek 0ml
@07:53:14.03 delay 10 seconds
@07:53:24.07 ATV.dial :air3
@07:53:25.67 AS.seek 0.01ml
@07:53:27.99 delay 2 seconds
@07:53:30.02 ATV.dial :bleach
@07:53:30.69 AS.seek 0.58ml
@07:55:25.58 delay 3 seconds
@07:55:28.61 ATV.dial :air3
@07:55:29.20 delay 3 seconds
@07:55:32.27 AS.seek 0.605ml
@07:55:37.70 delay 3 seconds
@07:55:40.73 ATV.dial :bleach
@07:55:41.37 AS.seek 0.59ml
@07:55:44.66 delay 3 seconds
@07:55:47.69 ATV.dial ASV
@07:55:48.78 ASV.dial :SPEdelivery
@07:55:49.15 AS.seek 0ml
@07:57:48.24 delay 1:00
@07:58:48.28 AFV.select :carrierSolution
@07:58:48.38 delay 2 seconds
@07:58:50.40 AS.reconfigure ASconfig
@07:58:50.46 AS.seek 1ml
@07:59:48.25 delay 1 second
@07:59:49.28 AFV.select :holdingCoil
@07:59:49.38 delay 1 second
@07:59:50.40 ATV.dial ASV
@07:59:50.78 ASV.dial :SPEdelivery
@07:59:50.88 AS.reconfigure ASslow1
@07:59:50.94 AS.seek 0ml
@08:03:12.46 delay 10 seconds
@08:03:22.49 ATV.dial :air2
@08:03:23.39 AS.reconfigure ASslow0
@08:03:23.45 AS.seek 0.8ml
@08:04:44.23 delay 3 seconds
@08:04:47.25 ATV.dial :ASV
@08:04:48.32 ASV.dial :SPEdelivery
@08:04:48.34 AS.reconfigure ASslow1
@08:04:48.40 AS.seek 0.03ml
@08:07:23.54 delay 20 seconds
@08:07:43.57 ATV.dial :waste,via: :air3
@08:07:45.42 AS.reconfigure ASconfig
@08:07:45.48 AS.seek 0ml
@08:07:47.48 SPE.coast
@08:07:47.57 Removing Bleach from Mixing Coil
AFV.select :carrierSolution
@08:07:47.69 delay 2 seconds
@08:07:49.71 AS.seek 0.5ml
@08:08:18.78 delay 1 second
@08:08:19.81 AFV.select :holdingCoil
@08:08:19.91 ATV.dial :ASV
@08:08:21.70 ASV.dial :SPErecovery
@08:08:22.36 AS.reconfigure ASslow1
@08:08:22.42 AS.seek 0.73ml
@08:09:09.00 ASV.dial :wasteD
@08:09:09.60 AS.reconfigure ASnorm
@08:09:09.65 AS.seek 0ml
@08:09:46.78 AFV.select :carrierSolution
@08:09:46.90 delay 2 seconds
@08:09:48.92 AS.reconfigure ASconfig
@08:09:48.98 AS.seek 0.5ml
@08:10:18.00 delay 1 second
@08:10:19.03 AFV.select :holdingCoil
@08:10:19.13 ATV.dial ASV
@08:10:19.15 ASV.dial :SPErecovery
@08:10:19.81 AS.reconfigure ASslow0
@08:10:19.89 AS.seek 0.3ml
@08:10:40.28 ATV.dial :air2
@08:10:41.39 AS.reconfigure ASslow1
@08:10:41.45 AS.seek 0.32ml
@08:10:45.87 ATV.dial ASV
@08:10:46.95 AS.reconfigure ASslow0
@08:10:47.01 AS.seek 0.57ml
@08:11:12.52 ASV.dial :wasteD
@08:11:13.14 AS.reconfigure ASnorm
@08:11:13.20 AS.seek 0ml
@08:11:42.22 AFV.select :carrierSolution
@08:11:42.33 delay 2 seconds
@08:11:44.35 AS.reconfigure ASconfig
@08:11:44.41 AS.seek 0.5ml
@08:12:13.45 delay 1 second
@08:12:14.48 AFV.select :holdingCoil
@08:12:14.58 ATV.dial ASV
@08:12:14.59 ASV.dial :SPErecovery
@08:12:15.26 AS.reconfigure ASslow0
@08:12:15.32 AS.seek 0.3ml
@08:12:35.77 ATV.dial :air2
@08:12:36.86 AS.reconfigure ASslow1
@08:12:36.92 AS.seek 0.32ml
@08:12:41.22 ATV.dial ASV
@08:12:42.31 AS.reconfigure ASslow0
@08:12:42.36 AS.seek 0.57ml
@08:13:07.92 ASV.dial :wasteD
@08:13:08.53 AS.reconfigure ASnorm
@08:13:08.58 AS.seek 0ml
@08:13:37.67 ATV.dial :air2
@08:13:38.70 AS.reconfigure ASslow0
@08:13:38.75 AS.seek 0.3ml
@08:14:09.30 ATV.dial :ASV
@08:14:10.34 ASV.dial :wasteD
@08:14:10.36 AS.reconfigure ASconfig
@08:14:10.42 AS.seek 0.05ml
@08:14:25.09 ATV.dial :waste
@08:14:27.09 AS.seek 0ml
@08:14:30.24 ASV.dial :SPEdelivery
@08:14:31.33 Cleaning PCR
@08:14:31.34 AFV.select :carrierSolution
@08:14:31.45 delay 2 seconds
@08:14:33.47 AS.seek 0.5ml
@08:15:02.48 delay 1 second
@08:15:03.51 AFV.select :holdingCoil
@08:15:03.61 ASV.dial :waste1
@08:15:03.98 ATV.dial :air3
@08:15:04.70 AS.reconfigure ASslow1
@08:15:04.76 AS.seek 0.51ml
@08:15:07.03 delay 2 seconds
@08:15:09.06 ATV.dial :bleach
@08:15:09.53 AS.seek 0.81ml
@08:16:10.23 delay 3 seconds
@08:16:13.26 ATV.dial :air3
@08:16:13.85 delay 3 seconds
@08:16:16.92 AS.seek 0.835ml
@08:16:22.31 delay 3 seconds
@08:16:25.34 ATV.dial :bleach
@08:16:25.97 AS.seek 0.82ml
@08:16:29.27 delay 3 seconds
@08:16:32.30 ARV.dial :waste
@08:16:32.93 delay 3 seconds
@08:16:35.95 ATV.dial :ARV
@08:16:36.89 AS.reconfigure ASconfig
@08:16:36.94 AS.seek 0.47ml
@08:16:57.80 AFV.select :carrierSolution
@08:16:57.92 delay 2 seconds
@08:16:59.94 AS.seek 1ml
@08:17:30.74 delay 1 second
@08:17:31.76 AFV.select :holdingCoil
@08:17:31.86 delay 1 second
@08:17:32.88 AS.reconfigure ASfast
@08:17:32.94 AS.seek 0ml
@08:18:00.27 AFV.select :carrierSolution
@08:18:00.39 delay 2 seconds
@08:18:02.41 AS.reconfigure ASconfig
@08:18:02.47 AS.seek 1ml
@08:19:00.26 delay 1 second
@08:19:01.28 AFV.select :holdingCoil
@08:19:01.38 delay 1 second
@08:19:02.40 AS.reconfigure ASfast
@08:19:02.46 AS.seek 0ml
@08:19:29.80 AFV.select :carrierSolution
@08:19:29.92 delay 2 seconds
@08:19:31.94 AS.reconfigure ASconfig
@08:19:32.00 AS.seek 1ml
@08:20:29.78 delay 1 second
@08:20:30.81 AFV.select :holdingCoil
@08:20:30.91 delay 1 second
@08:20:31.93 AS.reconfigure ASfast
@08:20:31.99 AS.seek 0ml
@08:20:59.33 ARV.dial between carrier and air1
@08:20:59.87 ATV.dial :air3
@08:21:01.22 AS.reconfigure ASslow1
@08:21:01.28 AS.seek 0.01ml
@08:21:03.58 delay 2 seconds
@08:21:05.61 ATV.dial :bleach
@08:21:06.25 AS.seek 0.81ml
@08:23:47.61 delay 3 seconds
@08:23:50.64 ATV.dial :air3
@08:23:51.23 delay 3 seconds
@08:23:54.30 AS.seek 0.835ml
@08:23:59.68 delay 3 seconds
@08:24:02.71 ATV.dial :bleach
@08:24:03.33 AS.seek 0.82ml
@08:24:06.62 delay 3 seconds
@08:24:09.65 ATV.dial PCR
@08:24:10.07 AS.reconfigure ASconfig
@08:24:10.12 AS.seek 0ml
@08:24:57.52 PCR.program :bleachProfile
@08:24:57.59 PCR program should complete in < 2:45
@08:24:57.60 PCR.run 10:00
@08:27:53.39 PCR.off
@08:27:53.44 AFV.select :carrierSolution
@08:27:53.55 delay 2 seconds
@08:27:55.57 AS.seek 1ml
@08:28:53.34 delay 1 second
@08:28:54.37 AFV.select :holdingCoil
@08:28:54.47 delay 1 second
@08:28:55.49 AS.reconfigure ASfast
@08:28:55.54 AS.seek 0ml
@08:29:22.87 delay 3 seconds
@08:29:25.90 AFV.select :carrierSolution
@08:29:26.00 delay 2 seconds
@08:29:28.02 AS.reconfigure ASconfig
@08:29:28.08 AS.seek 1ml
@08:30:25.93 delay 1 second
@08:30:26.95 AFV.select :holdingCoil
@08:30:27.05 delay 1 second
@08:30:28.07 AS.reconfigure ASfast
@08:30:28.13 AS.seek 0ml
@08:30:55.46 delay 3 seconds
@08:30:58.48 AFV.select :carrierSolution
@08:30:58.58 delay 2 seconds
@08:31:00.60 AS.reconfigure ASconfig
@08:31:00.66 AS.seek 1ml
@08:31:58.42 delay 1 second
@08:31:59.44 AFV.select :holdingCoil
@08:31:59.54 delay 1 second
@08:32:00.56 AS.reconfigure ASfast
@08:32:00.62 AS.seek 0ml
@08:32:27.96 delay 3 seconds
@08:32:30.98 AFV.select :carrierSolution
@08:32:31.08 delay 2 seconds
@08:32:33.10 AS.reconfigure ASconfig
@08:32:33.16 AS.seek 1ml
@08:33:30.96 delay 1 second
@08:33:31.98 AFV.select :holdingCoil
@08:33:32.08 delay 1 second
@08:33:33.10 AS.reconfigure ASfast
@08:33:33.16 AS.seek 0ml
@08:34:00.51 delay 3 seconds
@08:34:03.53 AFV.select :carrierSolution
@08:34:03.63 delay 2 seconds
@08:34:05.65 AS.reconfigure ASconfig
@08:34:05.72 AS.seek 1ml
@08:35:03.50 delay 1 second
@08:35:04.53 AFV.select :holdingCoil
@08:35:04.63 delay 1 second
@08:35:05.65 AS.reconfigure ASnorm
@08:35:05.71 AS.seek 0ml
@08:35:56.41 AFV.select :carrierSolution
@08:35:56.52 delay 2 seconds
@08:35:58.54 AS.reconfigure ASconfig
@08:35:58.59 AS.seek 1ml
@08:36:56.33 delay 1 second
@08:36:57.36 AFV.select :holdingCoil
@08:36:57.46 delay 1 second
@08:36:58.48 ATV.dial :waste
@08:36:59.76 AS.reconfigure ASnorm
@08:36:59.81 AS.seek 0.5ml
@08:37:25.37 ATV.dial :waste2
@08:37:26.74 AS.seek 0ml
@08:37:52.31 <MAIN> Cleaning PCR
@08:37:52.32 AFV.select :carrierSolution
@08:37:52.44 delay 2 seconds
@08:37:54.46 AS.reconfigure ASconfig
@08:37:54.51 AS.seek 0.5ml
@08:38:23.53 delay 1 second
@08:38:24.56 AFV.select :holdingCoil
@08:38:24.66 ASV.dial :waste1
@08:38:24.67 ATV.dial :air3
@08:38:26.75 AS.reconfigure ASslow1
@08:38:26.80 AS.seek 0.51ml
@08:38:29.09 delay 2 seconds
@08:38:31.11 ATV.dial :bleach
@08:38:31.58 AS.seek 0.81ml
@08:39:32.17 delay 3 seconds
@08:39:35.19 ATV.dial :air3
@08:39:35.78 delay 3 seconds
@08:39:38.85 AS.seek 0.835ml
@08:39:44.11 delay 3 seconds
@08:39:47.14 ATV.dial :bleach
@08:39:47.76 AS.seek 0.82ml
@08:39:51.09 delay 3 seconds
@08:39:54.12 ARV.dial :waste
@08:39:54.65 delay 3 seconds
@08:39:57.67 ATV.dial :ARV
@08:39:58.61 AS.reconfigure ASconfig
@08:39:58.66 AS.seek 0.47ml
@08:40:19.06 AFV.select :carrierSolution
@08:40:19.18 delay 2 seconds
@08:40:21.20 AS.seek 1ml
@08:40:51.98 delay 1 second
@08:40:53.01 AFV.select :holdingCoil
@08:40:53.11 delay 1 second
@08:40:54.13 AS.reconfigure ASfast
@08:40:54.18 AS.seek 0ml
@08:41:21.52 AFV.select :carrierSolution
@08:41:21.64 delay 2 seconds
@08:41:23.66 AS.reconfigure ASconfig
@08:41:23.71 AS.seek 1ml
@08:42:21.50 delay 1 second
@08:42:22.53 AFV.select :holdingCoil
@08:42:22.63 delay 1 second
@08:42:23.65 AS.reconfigure ASfast
@08:42:23.70 AS.seek 0ml
@08:42:51.03 AFV.select :carrierSolution
@08:42:51.15 delay 2 seconds
@08:42:53.17 AS.reconfigure ASconfig
@08:42:53.22 AS.seek 1ml
@08:43:51.01 delay 1 second
@08:43:52.04 AFV.select :holdingCoil
@08:43:52.14 delay 1 second
@08:43:53.16 AS.reconfigure ASfast
@08:43:53.21 AS.seek 0ml
@08:44:20.55 ARV.dial between carrier and air1
@08:44:21.09 ATV.dial :air3
@08:44:22.45 AS.reconfigure ASslow1
@08:44:22.50 AS.seek 0.01ml
@08:44:24.85 delay 2 seconds
@08:44:26.87 ATV.dial :bleach
@08:44:27.51 AS.seek 0.81ml
@08:47:08.66 delay 3 seconds
@08:47:11.69 ATV.dial :air3
@08:47:12.28 delay 3 seconds
@08:47:15.35 AS.seek 0.835ml
@08:47:20.63 delay 3 seconds
@08:47:23.66 ATV.dial :bleach
@08:47:24.29 AS.seek 0.82ml
@08:47:27.61 delay 3 seconds
@08:47:30.64 ATV.dial PCR
@08:47:31.06 AS.reconfigure ASconfig
@08:47:31.11 AS.seek 0ml
@08:48:18.55 PCR.program :bleachProfile
@08:48:18.63 PCR program should complete in < 2:45
@08:48:18.64 PCR.run 10:00
@08:51:14.86 PCR.off
@08:51:14.90 AFV.select :carrierSolution
@08:51:15.01 delay 2 seconds
@08:51:17.03 AS.seek 1ml
@08:52:14.80 delay 1 second
@08:52:15.83 AFV.select :holdingCoil
@08:52:15.93 delay 1 second
@08:52:16.95 AS.reconfigure ASfast
@08:52:17.00 AS.seek 0ml
@08:52:44.35 delay 3 seconds
@08:52:47.37 AFV.select :carrierSolution
@08:52:47.47 delay 2 seconds
@08:52:49.49 AS.reconfigure ASconfig
@08:52:49.55 AS.seek 1ml
@08:53:47.33 delay 1 second
@08:53:48.36 AFV.select :holdingCoil
@08:53:48.46 delay 1 second
@08:53:49.48 AS.reconfigure ASfast
@08:53:49.54 AS.seek 0ml
@08:54:16.88 delay 3 seconds
@08:54:19.90 AFV.select :carrierSolution
@08:54:20.00 delay 2 seconds
@08:54:22.02 AS.reconfigure ASconfig
@08:54:22.09 AS.seek 1ml
@08:55:19.87 delay 1 second
@08:55:20.90 AFV.select :holdingCoil
@08:55:21.00 delay 1 second
@08:55:22.02 AS.reconfigure ASfast
@08:55:22.07 AS.seek 0ml
@08:55:49.41 delay 3 seconds
@08:55:52.44 AFV.select :carrierSolution
@08:55:52.54 delay 2 seconds
@08:55:54.56 AS.reconfigure ASconfig
@08:55:54.63 AS.seek 1ml
@08:56:52.43 delay 1 second
@08:56:53.45 AFV.select :holdingCoil
@08:56:53.55 delay 1 second
@08:56:54.57 AS.reconfigure ASfast
@08:56:54.62 AS.seek 0ml
@08:57:21.95 delay 3 seconds
@08:57:24.98 AFV.select :carrierSolution
@08:57:25.08 delay 2 seconds
@08:57:27.10 AS.reconfigure ASconfig
@08:57:27.15 AS.seek 1ml
@08:58:24.94 delay 1 second
@08:58:25.97 AFV.select :holdingCoil
@08:58:26.07 delay 1 second
@08:58:27.09 AS.reconfigure ASnorm
@08:58:27.14 AS.seek 0ml
@08:59:17.88 delay 5 seconds
@08:59:22.96 Cleaning PCR
@08:59:22.97 AFV.select :carrierSolution
@08:59:23.09 delay 2 seconds
@08:59:25.11 AS.reconfigure ASconfig
@08:59:25.16 AS.seek 0.5ml
@08:59:54.20 delay 1 second
@08:59:55.23 AFV.select :holdingCoil
@08:59:55.33 ASV.dial :waste1
@08:59:55.34 ATV.dial :air3
@08:59:56.23 AS.reconfigure ASslow1
@08:59:56.28 AS.seek 0.51ml
@08:59:58.66 delay 2 seconds
@09:00:00.69 ATV.dial :bleach
@09:00:01.32 AS.seek 0.81ml
@09:00:48.65 <mwj#2> Session Begun
@09:00:58.10 -> showlog 10
@09:01:02.04 <MAIN> delay 3 seconds
@09:01:05.06 ATV.dial :air3
@09:01:05.65 delay 3 seconds
@09:01:08.72 AS.seek 0.835ml
@09:01:11.01 <mwj#2> -> showlog 100
@09:01:14.12 <MAIN> delay 3 seconds
@09:01:17.15 ATV.dial :bleach
@09:01:17.78 AS.seek 0.82ml
@09:01:21.08 delay 3 seconds
@09:01:24.11 ARV.dial :waste
@09:01:24.63 delay 3 seconds
@09:01:26.90 <mwj#2> -> showlog 500
@09:01:27.65 <MAIN> ATV.dial :ARV
@09:01:28.64 AS.reconfigure ASconfig
@09:01:29.06 AS.seek 0.47ml
@09:01:49.47 AFV.select :carrierSolution
@09:01:49.59 delay 2 seconds
@09:01:51.61 AS.seek 1ml
@09:01:53.69 <mwj#2> -> exit
@09:01:53.70 Session Ended
@09:02:22.36 <MAIN> delay 1 second
@09:02:23.38 AFV.select :holdingCoil
@09:02:23.48 delay 1 second
@09:02:24.50 AS.reconfigure ASfast
@09:02:24.57 AS.seek 0ml
@09:02:51.91 AFV.select :carrierSolution
@09:02:52.03 delay 2 seconds
@09:02:54.05 AS.reconfigure ASconfig
@09:02:54.11 AS.seek 1ml
@09:02:59.49 <mwj#2> Session Begun
@09:03:10.17 -> showlog 100
@09:03:51.90 <MAIN> delay 1 second
@09:03:52.92 AFV.select :holdingCoil
@09:03:53.02 delay 1 second
@09:03:54.04 AS.reconfigure ASfast
@09:03:54.11 AS.seek 0ml
@09:04:21.45 AFV.select :carrierSolution
@09:04:21.58 delay 2 seconds
@09:04:23.60 AS.reconfigure ASconfig
@09:04:23.67 AS.seek 1ml
@09:05:04.30 <mwj#2> -> exit
@09:05:04.32 Session Ended
@09:05:21.43 <MAIN> delay 1 second
@09:05:22.45 AFV.select :holdingCoil
@09:05:22.55 delay 1 second
@09:05:23.57 AS.reconfigure ASfast
@09:05:23.62 AS.seek 0ml
@09:05:50.95 ARV.dial between carrier and air1
@09:05:51.48 ATV.dial :air3
@09:05:52.84 AS.reconfigure ASslow1
@09:05:52.89 AS.seek 0.01ml
@09:05:55.25 delay 2 seconds
@09:05:57.28 ATV.dial :bleach
@09:05:57.93 AS.seek 0.81ml
@09:08:39.07 delay 3 seconds
@09:08:42.09 ATV.dial :air3
@09:08:42.68 delay 3 seconds
@09:08:45.75 AS.seek 0.835ml
@09:08:51.07 delay 3 seconds
@09:08:54.09 ATV.dial :bleach
@09:08:54.73 AS.seek 0.82ml
@09:08:58.01 delay 3 seconds
@09:09:01.04 ATV.dial PCR
@09:09:01.46 AS.reconfigure ASconfig
@09:09:01.51 AS.seek 0ml
@09:09:48.92 PCR.program :bleachProfile
@09:09:48.99 PCR program should complete in < 2:45
@09:09:49.00 PCR.run 10:00
@09:12:47.82 PCR.off
@09:12:47.86 AFV.select :carrierSolution
@09:12:47.97 delay 2 seconds
@09:12:49.99 AS.seek 1ml
@09:13:47.78 delay 1 second
@09:13:48.81 AFV.select :holdingCoil
@09:13:48.91 delay 1 second
@09:13:49.93 AS.reconfigure ASfast
@09:13:49.98 AS.seek 0ml
@09:14:17.31 delay 3 seconds
@09:14:20.34 AFV.select :carrierSolution
@09:14:20.44 delay 2 seconds
@09:14:22.46 AS.reconfigure ASconfig
@09:14:22.52 AS.seek 1ml
@09:15:20.61 delay 1 second
@09:15:21.64 AFV.select :holdingCoil
@09:15:21.74 delay 1 second
@09:15:22.76 AS.reconfigure ASfast
@09:15:22.81 AS.seek 0ml
@09:15:50.15 delay 3 seconds
@09:15:53.17 AFV.select :carrierSolution
@09:15:53.27 delay 2 seconds
@09:15:55.29 AS.reconfigure ASconfig
@09:15:55.36 AS.seek 1ml
@09:16:53.14 delay 1 second
@09:16:54.17 AFV.select :holdingCoil
@09:16:54.27 delay 1 second
@09:16:55.29 AS.reconfigure ASfast
@09:16:55.35 AS.seek 0ml
@09:17:22.71 delay 3 seconds
@09:17:25.73 AFV.select :carrierSolution
@09:17:25.83 delay 2 seconds
@09:17:27.85 AS.reconfigure ASconfig
@09:17:27.91 AS.seek 1ml
@09:18:25.70 delay 1 second
@09:18:26.72 AFV.select :holdingCoil
@09:18:26.82 delay 1 second
@09:18:27.84 AS.reconfigure ASfast
@09:18:27.90 AS.seek 0ml
@09:18:55.24 delay 3 seconds
@09:18:58.26 AFV.select :carrierSolution
@09:18:58.36 delay 2 seconds
@09:19:00.38 AS.reconfigure ASconfig
@09:19:00.44 AS.seek 1ml
@09:19:58.22 delay 1 second
@09:19:59.25 AFV.select :holdingCoil
@09:19:59.35 delay 1 second
@09:20:00.37 AS.reconfigure ASnorm
@09:20:00.43 AS.seek 0ml
@09:20:51.14 delay 5 seconds
@09:20:56.21 Cleaning PCR
@09:20:56.22 AFV.select :carrierSolution
@09:20:56.34 delay 2 seconds
@09:20:58.36 AS.reconfigure ASconfig
@09:20:58.42 AS.seek 0.5ml
@09:21:27.40 delay 1 second
@09:21:28.43 AFV.select :holdingCoil
@09:21:28.54 ASV.dial :waste1
@09:21:28.55 ATV.dial :air3
@09:21:29.43 AS.reconfigure ASslow1
@09:21:29.48 AS.seek 0.51ml
@09:21:31.81 delay 2 seconds
@09:21:33.84 ATV.dial :bleach
@09:21:34.50 AS.seek 0.81ml
@09:22:35.22 delay 3 seconds
@09:22:38.25 ATV.dial :air3
@09:22:38.85 delay 3 seconds
@09:22:41.92 AS.seek 0.835ml
@09:22:47.31 delay 3 seconds
@09:22:50.34 ATV.dial :bleach
@09:22:50.98 AS.seek 0.82ml
@09:22:54.26 delay 3 seconds
@09:22:57.29 ARV.dial :waste
@09:22:57.81 delay 3 seconds
@09:23:00.83 ATV.dial :ARV
@09:23:01.75 AS.reconfigure ASconfig
@09:23:01.80 AS.seek 0.47ml
@09:23:22.20 AFV.select :carrierSolution
@09:23:22.32 delay 2 seconds
@09:23:24.34 AS.seek 1ml
@09:23:55.12 delay 1 second
@09:23:56.15 AFV.select :holdingCoil
@09:23:56.25 delay 1 second
@09:23:57.27 AS.reconfigure ASfast
@09:23:57.32 AS.seek 0ml
@09:24:24.65 AFV.select :carrierSolution
@09:24:24.77 delay 2 seconds
@09:24:26.79 AS.reconfigure ASconfig
@09:24:26.84 AS.seek 1ml
@09:25:24.66 delay 1 second
@09:25:25.68 AFV.select :holdingCoil
@09:25:25.78 delay 1 second
@09:25:26.80 AS.reconfigure ASfast
@09:25:26.86 AS.seek 0ml
@09:25:54.20 AFV.select :carrierSolution
@09:25:54.32 delay 2 seconds
@09:25:56.34 AS.reconfigure ASconfig
@09:25:56.39 AS.seek 1ml
@09:26:54.17 delay 1 second
@09:26:55.20 AFV.select :holdingCoil
@09:26:55.30 delay 1 second
@09:26:56.32 AS.reconfigure ASfast
@09:26:56.38 AS.seek 0ml
@09:27:23.72 ARV.dial between carrier and air1
@09:27:24.26 ATV.dial :air3
@09:27:25.61 AS.reconfigure ASslow1
@09:27:25.66 AS.seek 0.01ml
@09:27:28.06 delay 2 seconds
@09:27:30.08 ATV.dial :bleach
@09:27:30.71 AS.seek 0.81ml
@09:30:11.87 delay 3 seconds
@09:30:14.90 ATV.dial :air3
@09:30:15.49 delay 3 seconds
@09:30:18.56 AS.seek 0.835ml
@09:30:23.95 delay 3 seconds
@09:30:26.97 ATV.dial :bleach
@09:30:27.59 AS.seek 0.82ml
@09:30:30.87 delay 3 seconds
@09:30:33.90 ATV.dial PCR
@09:30:34.34 AS.reconfigure ASconfig
@09:30:34.40 AS.seek 0ml
@09:31:21.79 PCR.program :bleachProfile
@09:31:21.87 PCR program should complete in < 2:45
@09:31:21.88 PCR.run 10:00
@09:34:21.08 PCR.off
@09:34:21.12 AFV.select :carrierSolution
@09:34:21.23 delay 2 seconds
@09:34:23.25 AS.seek 1ml
@09:35:21.00 delay 1 second
@09:35:22.03 AFV.select :holdingCoil
@09:35:22.13 delay 1 second
@09:35:23.15 AS.reconfigure ASfast
@09:35:23.21 AS.seek 0ml
@09:35:50.54 delay 3 seconds
@09:35:53.56 AFV.select :carrierSolution
@09:35:53.66 delay 2 seconds
@09:35:55.68 AS.reconfigure ASconfig
@09:35:55.75 AS.seek 1ml
@09:36:53.50 delay 1 second
@09:36:54.53 AFV.select :holdingCoil
@09:36:54.63 delay 1 second
@09:36:55.65 AS.reconfigure ASfast
@09:36:55.71 AS.seek 0ml
@09:37:23.06 delay 3 seconds
@09:37:26.08 AFV.select :carrierSolution
@09:37:26.18 delay 2 seconds
@09:37:28.20 AS.reconfigure ASconfig
@09:37:28.25 AS.seek 1ml
@09:38:26.04 delay 1 second
@09:38:27.07 AFV.select :holdingCoil
@09:38:27.17 delay 1 second
@09:38:28.19 AS.reconfigure ASfast
@09:38:28.24 AS.seek 0ml
@09:38:55.59 delay 3 seconds
@09:38:58.61 AFV.select :carrierSolution
@09:38:58.71 delay 2 seconds
@09:39:00.73 AS.reconfigure ASconfig
@09:39:00.79 AS.seek 1ml
@09:39:58.58 delay 1 second
@09:39:59.61 AFV.select :holdingCoil
@09:39:59.71 delay 1 second
@09:40:00.73 AS.reconfigure ASfast
@09:40:00.78 AS.seek 0ml
@09:40:28.12 delay 3 seconds
@09:40:31.14 AFV.select :carrierSolution
@09:40:31.24 delay 2 seconds
@09:40:33.26 AS.reconfigure ASconfig
@09:40:33.33 AS.seek 1ml
@09:41:31.09 delay 1 second
@09:41:32.12 AFV.select :holdingCoil
@09:41:32.22 delay 1 second
@09:41:33.24 AS.reconfigure ASnorm
@09:41:33.29 AS.seek 0ml
@09:42:24.03 delay 5 seconds
@09:42:29.11 <mwj> Session Ended
@11:12:09.21CEST20-Apr-21 <MAIN> ESP2 v3.30 I2C Gateway v3.06 for core bus
@11:12:11.76 
Configuration for mer 7/20/20 brent@mbari.org
/home/esp/esp2/type/shallow/mer/configure.rb
@11:12:11.85 
Configuration for MFB3 by preston@mbari.org
/home/esp/esp2/type/mfb/MFB3/configure.rb
@11:12:16.57 Polling Can every 10:00
@11:12:18.27 CS.configure :default
@11:12:18.31 CS.hold
@11:12:18.36 CS.configure :default
@11:12:18.44 PS.configure :default
@11:12:18.52 SS.configure :default
@11:12:18.55 SS.hold
@11:12:18.62 SS.configure :default
@11:12:18.70 CC.configure :default
@11:12:18.73 CC.hold
@11:12:18.80 CC.configure :default
@11:12:18.88 PC.configure :default
@11:12:18.91 PC.hold
@11:12:18.98 PC.configure :default
@11:12:19.07 Forearm.configure :loose
@11:12:19.11 Forearm.hold
@11:12:19.16 Forearm.configure :loose
@11:12:19.23 Elbow.configure :loose
@11:12:19.27 Elbow.hold
@11:12:19.32 Elbow.configure :loose
@11:12:19.42 SC.configure :loose
@11:12:19.45 SC.hold
@11:12:19.50 SC.configure :loose
@11:12:19.58 SE.configure :default
@11:12:19.62 SE.hold
@11:12:19.68 SE.configure :default
@11:12:19.71 CH.coast
@11:12:19.76 CH.configure :default
@11:12:19.82 PH.coast
@11:12:19.92 PH.configure :default
@11:12:20.12 SPE.coast
@11:12:20.20 SPE.configure SPEconfig
@11:12:20.36 AS.configure ASconfig
@11:12:20.42 AS.hold
@11:12:20.52 AS.configure ASconfig
@11:12:20.60 PCR.off
@11:12:20.64 PCR.pidtemp= 50,0.1,0,300,25,25
@11:12:20.67 PCR.caltemp= 2.5051,0.0372,0
@11:12:20.70 All dwarf controllers running firmware version 3.75
@11:12:20.92 Cleanly exited at 11:11:51CEST20-Apr-21
@11:12:20.95 Loading /home/esp/esp2/mission/GENOMAP_v3.rb mission
@11:12:21.10 <MFBPCR> Extracting Nucleic Acid from extSrc
@11:12:21.12 SPE.hold 2:46:40,55C
@11:12:21.16 ASV.dial :SPEdelivery
@11:12:21.61 delay 20 seconds
@11:12:23.33 <mwj> Session Begun
@11:12:41.64 <MFBPCR> AFV.select :holdingCoil
@11:12:41.76 ATV.dial :air2
@11:12:43.05 AS.reconfigure ASslow1
@11:12:43.11 AS.seek 0.07ml
@11:12:57.47 delay 3 seconds
@11:13:00.49 ATV.dial :extSrc
@11:13:02.02 AS.seek 0.47ml
@11:14:22.77 delay 5 seconds
@11:14:27.84 Solid Phase Extraction Heater at 40.1C
@11:14:27.85 ATV.dial ASV
@11:14:28.72 ASV.dial :SPEdelivery
@11:14:28.81 AS.seek 0.042ml
@11:14:56.54 <mwj> -> exit
@11:14:56.56 Session Ended
@11:15:55.16 <MFBPCR> delay 10 seconds
@11:16:05.19 Rinsing external lysate line extSrc
@11:16:05.20 AFV.select :carrierSolution
@11:16:05.30 delay 2 seconds
@11:16:07.32 AS.reconfigure ASconfig
@11:16:07.37 AS.seek 1ml
@11:17:02.74 delay 1 second
@11:17:03.76 AFV.select :holdingCoil
@11:17:03.86 delay 1 second
@11:17:04.88 ATV.dial :extSrc
@11:17:05.69 AS.reconfigure ASnorm
@11:17:05.74 AS.seek 0ml
@11:17:56.44 ATV.dial :air2
@11:17:57.99 AS.reconfigure ASslow0
@11:17:58.04 AS.seek 0.05ml
@11:18:03.44 ATV.dial :extSrc
@11:18:05.01 AS.seek 0.01ml
@11:18:09.39 ATV.dial :waste
@11:18:11.08 AS.reconfigure ASconfig
@11:18:11.17 AS.seek 0ml
@11:18:12.02 ethanol wash
@11:18:12.03 ATV.dial :air2
@11:18:13.19 AS.reconfigure ASslow2
@11:18:13.23 AS.seek 0.01ml
@11:18:23.64 delay 3 seconds
@11:18:26.66 Pulling 0.4ml of Ethanol Wash
@11:18:26.67 ATV.dial :ethanol
@11:18:27.55 AS.reconfigure ASslow1
@11:18:27.59 AS.seek 0.41ml
@11:19:48.38 delay 3 seconds
@11:19:51.40 ATV.dial :air1
@11:19:51.82 delay 3 seconds
@11:19:54.89 AS.seek 0.435ml
@11:20:00.22 delay 3 seconds
@11:20:03.24 ATV.dial :ethanol
@11:20:03.86 AS.seek 0.42ml
@11:20:07.16 delay 3 seconds
@11:20:10.18 ATV.dial ASV
@11:20:11.60 AS.seek 0.093ml
@11:21:17.66 delay 3 seconds
@11:21:20.68 ATV.dial :waste2
@11:21:21.47 AS.reconfigure ASconfig
@11:21:21.52 AS.seek 0ml
@11:21:27.14 ATV.dial :air2
@11:21:27.54 AS.reconfigure ASslow1
@11:21:27.59 AS.seek 1ml
@11:24:48.96 delay 5 seconds
@11:24:53.98 ATV.dial ASV
@11:24:55.00 ASV.dial :SPEdelivery
@11:24:55.02 AS.seek 0.02ml
@11:28:12.53 delay 5 seconds
@11:28:17.55 ATV.dial :air2
@11:28:18.64 AS.seek 0.52ml
@11:29:59.49 delay 5 seconds
@11:30:04.51 ATV.dial ASV
@11:30:05.55 ASV.dial :wasteR
@11:30:06.00 AS.seek 0.3ml
@11:30:50.55 delay 20 seconds
@11:31:10.59 AS.seek 0.07ml
@11:31:57.24 delay 20 seconds
@11:32:17.26 ATV.dial :waste2
@11:32:18.05 AS.reconfigure ASconfig
@11:32:18.10 AS.seek 0ml
@11:32:22.43 Elution
@11:32:22.44 ATV.dial :air1
@11:32:23.62 AS.reconfigure ASslow1
@11:32:23.67 AS.seek 0.05ml
@11:32:34.10 delay 3 seconds
@11:32:37.12 ATV.dial :ElutionWater
@11:32:37.82 AS.seek 0.11ml
@11:32:50.25 delay 3 seconds
@11:32:53.27 ATV.dial ASV,via: :air2
@11:32:55.48 ASV.dial :SPEelution
@11:32:56.08 AFV.select :carrierSolution
@11:32:56.23 AS.reconfigure ASnorm
@11:32:56.27 AS.seek 0.76ml
@11:33:29.30 delay 3 seconds
@11:33:32.32 AFV.select :holdingCoil
@11:33:32.47 AS.reconfigure ASslow1
@11:33:32.51 AS.seek 0.475ml
@11:34:30.10 delay 5 seconds
@11:34:35.18 AS.reconfigure ASslow2
@11:34:35.22 AS.seek 0.44ml
@11:35:10.82 delay 1:00
@11:36:10.85 pushing eluate to holding coil
@11:36:10.91 AS.seek 0.28ml
@11:38:52.36 delay 30 seconds
@11:39:22.39 ATV.dial :waste,via: :air3
@11:39:24.22 AS.reconfigure ASconfig
@11:39:24.27 AS.seek 0ml
@11:39:40.63 Parking Bleach in LysateSource line and SPEcolumn
@11:39:40.64 ATV.dial :air3
@11:39:41.52 AS.reconfigure ASslow1
@11:39:41.57 AS.seek 0.03ml
@11:39:47.91 ATV.dial :ElutionWater
@11:39:48.54 AS.seek 0.06ml
@11:39:54.83 ATV.dial :air3
@11:39:55.47 AS.seek 0.07ml
@11:39:57.75 delay 2 seconds
@11:39:59.77 ATV.dial :bleach
@11:40:00.25 AS.seek 0.15ml
@11:40:16.61 delay 3 seconds
@11:40:19.63 ATV.dial :air3
@11:40:20.22 delay 3 seconds
@11:40:23.29 AS.seek 0.175ml
@11:40:28.49 delay 3 seconds
@11:40:31.51 ATV.dial :bleach
@11:40:32.15 AS.seek 0.16ml
@11:40:35.46 delay 3 seconds
@11:40:38.48 ATV.dial :extSrc
@11:40:39.11 AS.reconfigure ASconfig
@11:40:39.16 AS.seek 0.015ml
@11:40:47.77 ATV.dial :waste
@11:40:49.30 AS.seek 0ml
@11:40:50.44 ATV.dial :air3
@11:40:51.32 AS.reconfigure ASslow1
@11:40:51.37 AS.seek 0.01ml
@11:40:53.80 delay 2 seconds
@11:40:55.82 ATV.dial :bleach
@11:40:56.29 AS.seek 0.58ml
@11:42:51.24 delay 3 seconds
@11:42:54.26 ATV.dial :air3
@11:42:54.83 delay 3 seconds
@11:42:57.90 AS.seek 0.605ml
@11:43:03.23 delay 3 seconds
@11:43:06.25 ATV.dial :bleach
@11:43:06.89 AS.seek 0.59ml
@11:43:10.18 delay 3 seconds
@11:43:13.20 ATV.dial :ASV
@11:43:14.30 ASV.dial :wasteR
@11:43:14.91 AS.seek 0ml
@11:45:13.83 delay 1:00
@11:46:13.86 AFV.select :carrierSolution
@11:46:13.96 delay 2 seconds
@11:46:15.98 AS.reconfigure ASconfig
@11:46:16.03 AS.seek 1ml
@11:47:13.80 delay 1 second
@11:47:14.82 AFV.select :holdingCoil
@11:47:14.92 delay 1 second
@11:47:15.94 ATV.dial ASV
@11:47:15.95 ASV.dial :wasteR
@11:47:15.97 AS.reconfigure ASslow1
@11:47:16.01 AS.seek 0ml
@11:50:37.41 delay 20 seconds
@11:50:57.44 ATV.dial :air3
@11:50:59.05 AS.seek 0.59ml
@11:52:58.00 delay 3 seconds
@11:53:01.02 ATV.dial :ASV
@11:53:02.55 ASV.dial :wasteR
@11:53:02.62 AS.seek 0.09ml
@11:54:43.44 delay 30 seconds
@11:55:13.47 ATV.dial :extSrc
@11:55:14.28 delay 3 seconds
@11:55:17.30 ATV.dial :ElutionWater
@11:55:18.50 AS.seek 0.12ml
@11:55:24.83 ATV.dial :air3
@11:55:25.46 AS.seek 0.13ml
@11:55:27.75 delay 2 seconds
@11:55:29.77 ATV.dial :bleach
@11:55:30.25 AS.seek 0.23ml
@11:55:50.63 delay 3 seconds
@11:55:53.65 ATV.dial :air3
@11:55:54.25 delay 3 seconds
@11:55:57.32 AS.seek 0.255ml
@11:56:02.61 delay 3 seconds
@11:56:05.63 ATV.dial :bleach
@11:56:06.27 AS.seek 0.24ml
@11:56:09.58 delay 3 seconds
@11:56:12.60 ATV.dial :ASV
@11:56:14.11 ASV.dial :SPEdelivery
@11:56:14.50 AFV.select :carrierSolution
@11:56:14.60 delay 2 seconds
@11:56:16.64 AS.reconfigure ASconfig
@11:56:16.68 AS.seek 0.5ml
@11:56:31.92 delay 1 second
@11:56:32.94 AFV.select :holdingCoil
@11:56:33.09 AS.reconfigure ASslow1
@11:56:33.13 AS.seek 0.17ml
@11:57:39.89 SPE.hold 2:46:40,95C
@11:57:39.93 delay 3:00
@12:00:39.98 AFV.select :carrierSolution
@12:00:40.08 delay 2 seconds
@12:00:42.10 AS.reconfigure ASconfig
@12:00:42.15 AS.seek 1ml
@12:01:30.13 delay 1 second
@12:01:31.15 AFV.select :holdingCoil
@12:01:31.25 delay 1 second
@12:01:32.27 ASV.dial :wasteD
@12:01:33.39 AS.seek 0ml
@12:02:31.17 Priming Eluate
@12:02:31.18 AFV.select :carrierSolution
@12:02:31.28 delay 2 seconds
@12:02:33.31 AS.seek 0.5ml
@12:03:02.32 delay 1 second
@12:03:03.34 AFV.select :holdingCoil
@12:03:03.44 ATV.dial ASV
@12:03:03.45 ASV.dial :SPErecovery
@12:03:04.10 Swish #1 of 2
@12:03:04.16 AS.reconfigure ASslow0
@12:03:04.21 AS.seek 0.6ml
@12:03:14.65 AS.seek 0.5ml
@12:03:25.08 Swish #2 of 2
@12:03:25.14 AS.seek 0.6ml
@12:03:35.63 AS.seek 0.5ml
@12:03:46.05 delay 3 seconds
@12:03:49.12 AS.reconfigure ASslow1
@12:03:49.16 AS.seek 0.684ml
@12:04:26.47 delay 3 seconds
@12:04:29.49 Eluate primed
@12:04:29.50 ATV.dial :waste2
@12:04:30.16 AS.reconfigure ASnorm
@12:04:30.21 AS.seek 0ml
@12:05:05.00 SPE.coast
@12:05:05.06 ASV.dial :SPErecovery
@12:05:05.08 Setting up PCR for SYBRIPc -- writing /var/log/esp/SYBRIPc21apr2012h.pcr
@12:05:05.10 Building PCR Slug from ASV with MM1 mix, PP6 primer
@12:05:05.11 Priming with carrier
@12:05:05.12 ATV.dial ARV
@12:05:06.14 ARV.dial :carrier
@12:05:06.48 AS.seek 0.5ml
@12:05:32.05 AFV.select :carrierSolution
@12:05:32.15 delay 2 seconds
@12:05:34.18 AS.reconfigure ASconfig
@12:05:34.22 AS.seek 1ml
@12:06:03.24 delay 1 second
@12:06:04.26 AFV.select :holdingCoil
@12:06:04.36 delay 1 second
@12:06:05.38 ATV.dial :waste
@12:06:06.99 AS.reconfigure ASnorm
@12:06:07.04 AS.seek 0ml
@12:06:57.72 AFV.select :carrierSolution
@12:06:57.82 delay 2 seconds
@12:06:59.85 AS.reconfigure ASconfig
@12:06:59.89 AS.seek 0.5ml
@12:07:28.91 delay 1 second
@12:07:29.93 AFV.select :holdingCoil
@12:07:30.03 Building Slug
@12:07:30.05 ATV.dial :ARV
@12:07:31.80 delay 2 seconds
@12:07:33.82 ARV.dial :air1
@12:07:34.22 delay 2 seconds
@12:07:36.29 AS.reconfigure ASslow2
@12:07:36.33 AS.seek 0.506ml
@12:07:42.53 delay 2 seconds
@12:07:44.55 ARV.dial :MM1
@12:07:45.17 delay 2 seconds
@12:07:47.19 Adding MM1
@12:07:47.25 AS.seek 0.524ml
@12:08:05.58 delay 25 seconds
@12:08:30.60 ARV.dial :PP6
@12:08:31.92 Adding PP6
@12:08:31.93 delay 2 seconds
@12:08:34.00 AS.seek 0.53ml
@12:08:40.52 delay 10 seconds
@12:08:50.55 ARV.dial :air1
@12:08:51.41 adding ARV air
@12:08:51.42 delay 2 seconds
@12:08:53.49 AS.seek 0.536ml
@12:08:59.88 delay 5 seconds
@12:09:04.90 ARV.dial :carrier
@12:09:05.32 delay 7 seconds
@12:09:12.40 AS.seek 0.594ml
@12:10:11.00 delay 5 seconds
@12:10:16.02 ATV.dial :ASV
@12:10:16.44 delay 2 seconds
@12:10:18.51 AS.seek 0.6ml
@12:10:24.85 delay 4 seconds
@12:10:28.87 ATV.dial ARV
@12:10:29.42 delay 2 seconds
@12:10:31.44 ARV.dial :carrier
@12:10:31.45 delay 2 seconds
@12:10:33.52 AS.seek 0.65ml
@12:11:24.03 delay 4 seconds
@12:11:28.05 ATV.dial PCR
@12:11:28.69 ASV.dial :waste1
@12:11:29.49 ARV.dial :air1
@12:11:29.88 Positioning Slug by 0.324ml
@12:11:29.95 AS.seek 0.326ml
@12:16:55.83 delay 5 seconds
@12:17:00.85 ATV.dial ARV
@12:17:01.66 ASV.dial :PCRlock
@12:17:02.03 delay 5 seconds
@12:17:07.05 Running SYBRIPc Profile60CIQ1 thermocycling
@12:17:07.07 <PCR> PCR.program Profile60CIQ1
@12:17:07.17 Profile60CIQ1 should complete in < 1:56:15
@12:17:07.18 PCR.run 2:30:18.75
@13:56:34.66 PCR.off
@13:56:35.18 <MFBPCR> PCR for SYBRIPc completed -- closed /var/log/esp/SYBRIPc21apr2012h.pcr
@13:56:35.24 Cleaning PCR
@13:56:35.25 AFV.select :carrierSolution
@13:56:35.35 delay 2 seconds
@13:56:37.38 AS.reconfigure ASconfig
@13:56:37.42 AS.seek 0.5ml
@13:56:47.72 delay 1 second
@13:56:48.74 AFV.select :holdingCoil
@13:56:48.84 ASV.dial :waste1
@13:56:49.24 ATV.dial :air3
@13:56:50.58 AS.reconfigure ASslow1
@13:56:50.63 AS.seek 0.51ml
@13:56:52.89 delay 2 seconds
@13:56:54.91 ATV.dial :bleach
@13:56:55.55 AS.seek 0.81ml
@13:57:56.16 delay 3 seconds
@13:57:59.18 ATV.dial :air3
@13:57:59.75 delay 3 seconds
@13:58:02.82 AS.seek 0.835ml
@13:58:08.10 delay 3 seconds
@13:58:11.12 ATV.dial :bleach
@13:58:11.75 AS.seek 0.82ml
@13:58:15.06 delay 3 seconds
@13:58:18.08 ARV.dial :waste
@13:58:18.70 delay 3 seconds
@13:58:21.72 ATV.dial :ARV
@13:58:22.65 AS.reconfigure ASconfig
@13:58:22.70 AS.seek 0.47ml
@13:58:43.11 AFV.select :carrierSolution
@13:58:43.21 delay 2 seconds
@13:58:45.24 AS.seek 1ml
@13:59:16.02 delay 1 second
@13:59:17.04 AFV.select :holdingCoil
@13:59:17.14 delay 1 second
@13:59:18.17 AS.reconfigure ASfast
@13:59:18.21 AS.seek 0ml
@13:59:45.55 AFV.select :carrierSolution
@13:59:45.65 delay 2 seconds
@13:59:47.67 AS.reconfigure ASconfig
@13:59:47.72 AS.seek 1ml
@14:00:45.45 delay 1 second
@14:00:46.47 AFV.select :holdingCoil
@14:00:46.57 delay 1 second
@14:00:47.60 AS.reconfigure ASfast
@14:00:47.64 AS.seek 0ml
@14:01:14.97 AFV.select :carrierSolution
@14:01:15.07 delay 2 seconds
@14:01:17.43 AS.reconfigure ASconfig
@14:01:17.53 AS.seek 1ml
@14:02:15.27 delay 1 second
@14:02:16.29 AFV.select :holdingCoil
@14:02:16.39 delay 1 second
@14:02:17.42 AS.reconfigure ASfast
@14:02:17.46 AS.seek 0ml
@14:02:44.78 ARV.dial between carrier and air1
@14:02:45.30 ATV.dial :air3
@14:02:46.66 AS.reconfigure ASslow1
@14:02:46.70 AS.seek 0.01ml
@14:02:49.09 delay 2 seconds
@14:02:51.11 ATV.dial :bleach
@14:02:51.75 AS.seek 0.81ml
@14:05:32.91 delay 3 seconds
@14:05:35.93 ATV.dial :air3
@14:05:36.50 delay 3 seconds
@14:05:39.57 AS.seek 0.835ml
@14:05:44.89 delay 3 seconds
@14:05:47.91 ATV.dial :bleach
@14:05:48.56 AS.seek 0.82ml
@14:05:51.84 delay 3 seconds
@14:05:54.86 ATV.dial PCR
@14:05:55.27 AS.reconfigure ASconfig
@14:05:55.32 AS.seek 0ml
@14:06:42.72 PCR.program :bleachProfile
@14:06:42.78 PCR program should complete in < 2:45
@14:06:42.79 PCR.run 10:00
@14:09:38.55 PCR.off
@14:09:38.58 AFV.select :carrierSolution
@14:09:38.68 delay 2 seconds
@14:09:40.71 AS.seek 1ml
@14:10:38.44 delay 1 second
@14:10:39.46 AFV.select :holdingCoil
@14:10:39.56 delay 1 second
@14:10:40.59 AS.reconfigure ASfast
@14:10:40.63 AS.seek 0ml
@14:11:07.95 delay 3 seconds
@14:11:10.97 AFV.select :carrierSolution
@14:11:11.07 delay 2 seconds
@14:11:13.09 AS.reconfigure ASconfig
@14:11:13.14 AS.seek 1ml
@14:12:10.88 delay 1 second
@14:12:11.90 AFV.select :holdingCoil
@14:12:12.00 delay 1 second
@14:12:13.03 AS.reconfigure ASfast
@14:12:13.07 AS.seek 0ml
@14:12:40.41 delay 3 seconds
@14:12:43.43 AFV.select :carrierSolution
@14:12:43.53 delay 2 seconds
@14:12:45.55 AS.reconfigure ASconfig
@14:12:45.60 AS.seek 1ml
@14:13:43.34 delay 1 second
@14:13:44.36 AFV.select :holdingCoil
@14:13:44.46 delay 1 second
@14:13:45.49 AS.reconfigure ASfast
@14:13:45.53 AS.seek 0ml
@14:14:12.86 delay 3 seconds
@14:14:15.88 AFV.select :carrierSolution
@14:14:15.98 delay 2 seconds
@14:14:18.00 AS.reconfigure ASconfig
@14:14:18.05 AS.seek 1ml
@14:15:15.83 delay 1 second
@14:15:16.85 AFV.select :holdingCoil
@14:15:16.95 delay 1 second
@14:15:17.98 AS.reconfigure ASfast
@14:15:18.02 AS.seek 0ml
@14:15:45.34 delay 3 seconds
@14:15:48.36 AFV.select :carrierSolution
@14:15:48.46 delay 2 seconds
@14:15:50.48 AS.reconfigure ASconfig
@14:15:50.53 AS.seek 1ml
@14:16:48.31 delay 1 second
@14:16:49.33 AFV.select :holdingCoil
@14:16:49.43 delay 1 second
@14:16:50.46 AS.reconfigure ASnorm
@14:16:50.50 AS.seek 0ml
@14:17:41.26 Setting up PCR for ACYPP2 -- writing /var/log/esp/ACYPP221apr2014h.pcr
@14:17:41.28 Building PCR Slug from ASV with MM1 mix, PP2 primer
@14:17:41.29 Priming with carrier
@14:17:41.30 ATV.dial ARV
@14:17:41.92 ARV.dial :carrier
@14:17:42.26 AS.seek 0.5ml
@14:18:07.84 AFV.select :carrierSolution
@14:18:07.94 delay 2 seconds
@14:18:09.97 AS.reconfigure ASconfig
@14:18:10.01 AS.seek 1ml
@14:18:39.06 delay 1 second
@14:18:40.08 AFV.select :holdingCoil
@14:18:40.18 delay 1 second
@14:18:41.20 ATV.dial :waste
@14:18:42.97 AS.reconfigure ASnorm
@14:18:43.02 AS.seek 0ml
@14:19:33.70 AFV.select :carrierSolution
@14:19:34.22 delay 2 seconds
@14:19:36.25 AS.reconfigure ASconfig
@14:19:36.29 AS.seek 0.5ml
@14:20:05.36 delay 1 second
@14:20:06.38 AFV.select :holdingCoil
@14:20:06.48 Building Slug
@14:20:06.50 ATV.dial :ARV
@14:20:08.25 delay 2 seconds
@14:20:10.27 ARV.dial :air1
@14:20:10.66 delay 2 seconds
@14:20:12.73 AS.reconfigure ASslow2
@14:20:12.78 AS.seek 0.506ml
@14:20:18.97 delay 2 seconds
@14:20:20.99 ARV.dial :MM1
@14:20:21.63 delay 2 seconds
@14:20:23.65 Adding MM1
@14:20:23.71 AS.seek 0.524ml
@14:20:41.99 delay 25 seconds
@14:21:07.01 ARV.dial :PP2
@14:21:07.42 Adding PP2
@14:21:07.43 delay 2 seconds
@14:21:09.50 AS.seek 0.53ml
@14:21:16.16 delay 10 seconds
@14:21:26.19 ARV.dial :air2
@14:21:26.59 adding ARV air
@14:21:26.60 delay 2 seconds
@14:21:28.67 AS.seek 0.536ml
@14:21:34.92 delay 5 seconds
@14:21:39.94 ARV.dial :carrier
@14:21:41.27 delay 7 seconds
@14:21:48.35 AS.seek 0.594ml
@14:22:47.16 delay 5 seconds
@14:22:52.18 ATV.dial :ASV
@14:22:52.59 delay 2 seconds
@14:22:54.66 AS.seek 0.6ml
@14:23:01.14 delay 4 seconds
@14:23:05.16 ATV.dial ARV
@14:23:05.74 delay 2 seconds
@14:23:07.76 ARV.dial :carrier
@14:23:07.77 delay 2 seconds
@14:23:09.84 AS.seek 0.65ml
@14:24:00.59 delay 4 seconds
@14:24:04.61 ATV.dial PCR
@14:24:05.25 ASV.dial :waste1
@14:24:05.26 ARV.dial :air1
@14:24:05.67 Positioning Slug by 0.324ml
@14:24:05.74 AS.seek 0.326ml
@14:29:32.02 delay 5 seconds
@14:29:37.04 ATV.dial ARV
@14:29:37.84 ASV.dial :PCRlock
@14:29:38.24 delay 5 seconds
@14:29:43.26 Running ACYPP2 Profile60CIQ2 thermocycling
@14:29:43.28 <PCR> PCR.program Profile60CIQ2
@14:29:43.38 Profile60CIQ2 should complete in < 1:47:50
@14:29:43.39 PCR.run 2:19:47.5
@15:58:42.12 PCR.off
@15:58:42.71 <MFBPCR> Running ACYPP2 MeltProfile60 thermocycling
@15:58:42.72 <PCR> PCR.program MeltProfile60
@15:58:42.80 MeltProfile60 should complete in < 11:55
@15:58:42.81 PCR.run 19:53.75
@16:06:32.72 PCR.off
@16:06:33.98 <MFBPCR> PCR for ACYPP2 completed -- closed /var/log/esp/ACYPP221apr2014h.pcr
@16:06:34.04 Cleaning PCR
@16:06:34.05 AFV.select :carrierSolution
@16:06:34.15 delay 2 seconds
@16:06:36.18 AS.reconfigure ASconfig
@16:06:36.22 AS.seek 0.5ml
@16:06:46.48 delay 1 second
@16:06:47.50 AFV.select :holdingCoil
@16:06:47.60 ASV.dial :waste1
@16:06:48.00 ATV.dial :air3
@16:06:49.35 AS.reconfigure ASslow1
@16:06:49.40 AS.seek 0.51ml
@16:06:51.68 delay 2 seconds
@16:06:53.70 ATV.dial :bleach
@16:06:54.36 AS.seek 0.81ml
@16:07:55.05 delay 3 seconds
@16:07:58.07 ATV.dial :air3
@16:07:58.66 delay 3 seconds
@16:08:01.73 AS.seek 0.835ml
@16:08:07.04 delay 3 seconds
@16:08:10.06 ATV.dial :bleach
@16:08:10.70 AS.seek 0.82ml
@16:08:14.22 delay 3 seconds
@16:08:17.24 ARV.dial :waste
@16:08:17.86 delay 3 seconds
@16:08:20.88 ATV.dial :ARV
@16:08:21.80 AS.reconfigure ASconfig
@16:08:21.85 AS.seek 0.47ml
@16:08:42.25 AFV.select :carrierSolution
@16:08:42.35 delay 2 seconds
@16:08:44.38 AS.seek 1ml
@16:09:15.14 delay 1 second
@16:09:16.16 AFV.select :holdingCoil
@16:09:16.26 delay 1 second
@16:09:17.29 AS.reconfigure ASfast
@16:09:17.33 AS.seek 0ml
@16:09:44.66 AFV.select :carrierSolution
@16:09:44.76 delay 2 seconds
@16:09:46.78 AS.reconfigure ASconfig
@16:09:46.83 AS.seek 1ml
@16:10:44.56 delay 1 second
@16:10:45.58 AFV.select :holdingCoil
@16:10:45.68 delay 1 second
@16:10:46.71 AS.reconfigure ASfast
@16:10:46.75 AS.seek 0ml
@16:11:14.06 AFV.select :carrierSolution
@16:11:14.16 delay 2 seconds
@16:11:16.19 AS.reconfigure ASconfig
@16:11:16.23 AS.seek 1ml
@16:12:13.98 delay 1 second
@16:12:15.00 AFV.select :holdingCoil
@16:12:15.10 delay 1 second
@16:12:16.13 AS.reconfigure ASfast
@16:12:16.17 AS.seek 0ml
@16:12:43.50 ARV.dial between carrier and air1
@16:12:44.00 ATV.dial :air3
@16:12:45.33 AS.reconfigure ASslow1
@16:12:45.38 AS.seek 0.01ml
@16:12:47.84 delay 2 seconds
@16:12:49.86 ATV.dial :bleach
@16:12:50.50 AS.seek 0.81ml
@16:15:31.76 delay 3 seconds
@16:15:34.78 ATV.dial :air3
@16:15:35.37 delay 3 seconds
@16:15:38.44 AS.seek 0.835ml
@16:15:43.72 delay 3 seconds
@16:15:46.74 ATV.dial :bleach
@16:15:47.36 AS.seek 0.82ml
@16:15:50.64 delay 3 seconds
@16:15:53.66 ATV.dial PCR
@16:15:54.08 AS.reconfigure ASconfig
@16:15:54.13 AS.seek 0ml
@16:16:41.53 PCR.program :bleachProfile
@16:16:41.58 PCR program should complete in < 2:45
@16:16:41.59 PCR.run 10:00
@16:19:37.79 PCR.off
@16:19:37.82 AFV.select :carrierSolution
@16:19:37.92 delay 2 seconds
@16:19:39.95 AS.seek 1ml
@16:20:37.72 delay 1 second
@16:20:38.74 AFV.select :holdingCoil
@16:20:38.84 delay 1 second
@16:20:39.87 AS.reconfigure ASfast
@16:20:39.91 AS.seek 0ml
@16:21:07.25 delay 3 seconds
@16:21:10.27 AFV.select :carrierSolution
@16:21:10.37 delay 2 seconds
@16:21:12.40 AS.reconfigure ASconfig
@16:21:12.44 AS.seek 1ml
@16:22:10.22 delay 1 second
@16:22:11.24 AFV.select :holdingCoil
@16:22:11.34 delay 1 second
@16:22:12.37 AS.reconfigure ASfast
@16:22:12.41 AS.seek 0ml
@16:22:39.75 delay 3 seconds
@16:22:42.77 AFV.select :carrierSolution
@16:22:42.87 delay 2 seconds
@16:22:44.90 AS.reconfigure ASconfig
@16:22:44.94 AS.seek 1ml
@16:23:42.69 delay 1 second
@16:23:43.71 AFV.select :holdingCoil
@16:23:43.81 delay 1 second
@16:23:44.84 AS.reconfigure ASfast
@16:23:44.88 AS.seek 0ml
@16:24:12.22 delay 3 seconds
@16:24:15.24 AFV.select :carrierSolution
@16:24:15.34 delay 2 seconds
@16:24:17.37 AS.reconfigure ASconfig
@16:24:17.42 AS.seek 1ml
@16:25:15.16 delay 1 second
@16:25:16.18 AFV.select :holdingCoil
@16:25:16.28 delay 1 second
@16:25:17.31 AS.reconfigure ASfast
@16:25:17.35 AS.seek 0ml
@16:25:44.66 delay 3 seconds
@16:25:47.68 AFV.select :carrierSolution
@16:25:47.78 delay 2 seconds
@16:25:49.81 AS.reconfigure ASconfig
@16:25:49.85 AS.seek 1ml
@16:26:47.66 delay 1 second
@16:26:48.68 AFV.select :holdingCoil
@16:26:48.78 delay 1 second
@16:26:49.81 AS.reconfigure ASnorm
@16:26:49.85 AS.seek 0ml
@16:27:40.53 Setting up PCR for AOPP1 -- writing /var/log/esp/AOPP121apr2016h.pcr
@16:27:40.55 Building PCR Slug from ASV with MM1 mix, PP1 primer
@16:27:40.56 Priming with carrier
@16:27:40.57 ATV.dial ARV
@16:27:41.20 ARV.dial :carrier
@16:27:41.53 AS.seek 0.5ml
@16:28:07.08 AFV.select :carrierSolution
@16:28:07.18 delay 2 seconds
@16:28:09.21 AS.reconfigure ASconfig
@16:28:09.25 AS.seek 1ml
@16:28:38.26 delay 1 second
@16:28:39.28 AFV.select :holdingCoil
@16:28:39.38 delay 1 second
@16:28:40.40 ATV.dial :waste
@16:28:42.19 AS.reconfigure ASnorm
@16:28:42.24 AS.seek 0ml
@16:29:32.92 AFV.select :carrierSolution
@16:29:33.02 delay 2 seconds
@16:29:35.05 AS.reconfigure ASconfig
@16:29:35.09 AS.seek 0.5ml
@16:30:04.11 delay 1 second
@16:30:05.13 AFV.select :holdingCoil
@16:30:05.23 Building Slug
@16:30:05.24 ATV.dial :ARV
@16:30:07.00 delay 2 seconds
@16:30:09.02 ARV.dial :air1
@16:30:09.42 delay 2 seconds
@16:30:11.49 AS.reconfigure ASslow2
@16:30:11.53 AS.seek 0.506ml
@16:30:17.72 delay 2 seconds
@16:30:19.74 ARV.dial :MM1
@16:30:20.36 delay 2 seconds
@16:30:22.38 Adding MM1
@16:30:22.44 AS.seek 0.524ml
@16:30:40.92 delay 25 seconds
@16:31:05.94 ARV.dial :PP1
@16:31:06.33 Adding PP1
@16:31:06.34 delay 2 seconds
@16:31:08.41 AS.seek 0.53ml
@16:31:15.14 delay 10 seconds
@16:31:25.17 ARV.dial :air1
@16:31:25.58 adding ARV air
@16:31:25.59 delay 2 seconds
@16:31:27.66 AS.seek 0.536ml
@16:31:34.44 delay 5 seconds
@16:31:39.46 ARV.dial :carrier
@16:31:39.86 delay 7 seconds
@16:31:46.94 AS.seek 0.594ml
@16:32:45.36 delay 5 seconds
@16:32:50.38 ATV.dial :ASV
@16:32:50.80 delay 2 seconds
@16:32:52.87 AS.seek 0.6ml
@16:32:59.15 delay 4 seconds
@16:33:03.17 ATV.dial ARV
@16:33:03.75 delay 2 seconds
@16:33:05.77 ARV.dial :carrier
@16:33:05.78 delay 2 seconds
@16:33:07.85 AS.seek 0.65ml
@16:33:58.62 delay 4 seconds
@16:34:02.64 ATV.dial PCR
@16:34:03.28 ASV.dial :waste1
@16:34:03.69 ARV.dial :air1
@16:34:04.11 Positioning Slug by 0.324ml
@16:34:04.18 AS.seek 0.326ml
@16:39:30.37 delay 5 seconds
@16:39:35.39 ATV.dial ARV
@16:39:36.20 ASV.dial :PCRlock
@16:39:36.59 delay 5 seconds
@16:39:41.61 Running AOPP1 Profile60CIQ1 thermocycling
@16:39:41.63 <PCR> PCR.program Profile60CIQ1
@16:39:41.73 Profile60CIQ1 should complete in < 1:56:15
@16:39:41.74 PCR.run 2:30:18.75
@18:19:33.62 PCR.off
@18:19:34.53 <MFBPCR> Running AOPP1 MeltProfile60 thermocycling
@18:19:34.54 <PCR> PCR.program MeltProfile60
@18:19:34.63 MeltProfile60 should complete in < 11:55
@18:19:34.64 PCR.run 19:53.75
@18:27:21.50 PCR.off
@18:27:22.39 <MFBPCR> PCR for AOPP1 completed -- closed /var/log/esp/AOPP121apr2016h.pcr
@18:27:22.45 Cleaning PCR
@18:27:22.46 AFV.select :carrierSolution
@18:27:22.56 delay 2 seconds
@18:27:24.59 AS.reconfigure ASconfig
@18:27:24.63 AS.seek 0.5ml
@18:27:34.95 delay 1 second
@18:27:35.97 AFV.select :holdingCoil
@18:27:36.07 ASV.dial :waste1
@18:27:36.46 ATV.dial :air3
@18:27:37.80 AS.reconfigure ASslow1
@18:27:37.85 AS.seek 0.51ml
@18:27:40.13 delay 2 seconds
@18:27:42.15 ATV.dial :bleach
@18:27:42.78 AS.seek 0.81ml
@18:28:43.39 delay 3 seconds
@18:28:46.41 ATV.dial :air3
@18:28:46.98 delay 3 seconds
@18:28:50.05 AS.seek 0.835ml
@18:28:55.43 delay 3 seconds
@18:28:58.45 ATV.dial :bleach
@18:28:59.08 AS.seek 0.82ml
@18:29:02.51 delay 3 seconds
@18:29:05.53 ARV.dial :waste
@18:29:06.15 delay 3 seconds
@18:29:09.17 ATV.dial :ARV
@18:29:10.09 AS.reconfigure ASconfig
@18:29:10.14 AS.seek 0.47ml
@18:29:30.56 AFV.select :carrierSolution
@18:29:30.66 delay 2 seconds
@18:29:32.69 AS.seek 1ml
@18:30:03.40 delay 1 second
@18:30:04.42 AFV.select :holdingCoil
@18:30:04.52 delay 1 second
@18:30:05.55 AS.reconfigure ASfast
@18:30:05.59 AS.seek 0ml
@18:30:32.92 AFV.select :carrierSolution
@18:30:33.02 delay 2 seconds
@18:30:35.04 AS.reconfigure ASconfig
@18:30:35.09 AS.seek 1ml
@18:31:32.84 delay 1 second
@18:31:33.86 AFV.select :holdingCoil
@18:31:33.96 delay 1 second
@18:31:34.99 AS.reconfigure ASfast
@18:31:35.03 AS.seek 0ml
@18:32:02.35 AFV.select :carrierSolution
@18:32:02.45 delay 2 seconds
@18:32:04.47 AS.reconfigure ASconfig
@18:32:04.52 AS.seek 1ml
@18:33:02.28 delay 1 second
@18:33:03.30 AFV.select :holdingCoil
@18:33:03.40 delay 1 second
@18:33:04.43 AS.reconfigure ASfast
@18:33:04.47 AS.seek 0ml
@18:33:31.81 ARV.dial between carrier and air1
@18:33:32.31 ATV.dial :air3
@18:33:33.65 AS.reconfigure ASslow1
@18:33:33.70 AS.seek 0.01ml
@18:33:35.98 delay 2 seconds
@18:33:38.00 ATV.dial :bleach
@18:33:38.65 AS.seek 0.81ml
@18:36:19.78 delay 3 seconds
@18:36:22.80 ATV.dial :air3
@18:36:23.37 delay 3 seconds
@18:36:26.44 AS.seek 0.835ml
@18:36:31.73 delay 3 seconds
@18:36:34.75 ATV.dial :bleach
@18:36:35.38 AS.seek 0.82ml
@18:36:38.67 delay 3 seconds
@18:36:41.69 ATV.dial PCR
@18:36:42.09 AS.reconfigure ASconfig
@18:36:42.14 AS.seek 0ml
@18:37:29.54 PCR.program :bleachProfile
@18:37:29.59 PCR program should complete in < 2:45
@18:37:29.60 PCR.run 10:00
@18:40:25.78 PCR.off
@18:40:25.81 AFV.select :carrierSolution
@18:40:25.91 delay 2 seconds
@18:40:27.94 AS.seek 1ml
@18:41:25.67 delay 1 second
@18:41:26.69 AFV.select :holdingCoil
@18:41:26.79 delay 1 second
@18:41:27.82 AS.reconfigure ASfast
@18:41:27.86 AS.seek 0ml
@18:41:55.21 delay 3 seconds
@18:41:58.23 AFV.select :carrierSolution
@18:41:58.33 delay 2 seconds
@18:42:00.35 AS.reconfigure ASconfig
@18:42:00.41 AS.seek 1ml
@18:42:58.17 delay 1 second
@18:42:59.19 AFV.select :holdingCoil
@18:42:59.29 delay 1 second
@18:43:00.32 AS.reconfigure ASfast
@18:43:00.36 AS.seek 0ml
@18:43:27.70 delay 3 seconds
@18:43:30.72 AFV.select :carrierSolution
@18:43:30.82 delay 2 seconds
@18:43:32.85 AS.reconfigure ASconfig
@18:43:32.90 AS.seek 1ml
@18:44:30.64 delay 1 second
@18:44:31.66 AFV.select :holdingCoil
@18:44:31.76 delay 1 second
@18:44:32.79 AS.reconfigure ASfast
@18:44:32.83 AS.seek 0ml
@18:45:00.18 delay 3 seconds
@18:45:03.20 AFV.select :carrierSolution
@18:45:03.30 delay 2 seconds
@18:45:05.33 AS.reconfigure ASconfig
@18:45:05.38 AS.seek 1ml
@18:46:03.17 delay 1 second
@18:46:04.19 AFV.select :holdingCoil
@18:46:04.29 delay 1 second
@18:46:05.32 AS.reconfigure ASfast
@18:46:05.36 AS.seek 0ml
@18:46:32.70 delay 3 seconds
@18:46:35.72 AFV.select :carrierSolution
@18:46:35.82 delay 2 seconds
@18:46:37.84 AS.reconfigure ASconfig
@18:46:37.90 AS.seek 1ml
@18:47:35.67 delay 1 second
@18:47:36.69 AFV.select :holdingCoil
@18:47:36.79 delay 1 second
@18:47:37.82 AS.reconfigure ASnorm
@18:47:37.86 AS.seek 0ml
@18:48:28.58 Setting up PCR for AARPP4 -- writing /var/log/esp/AARPP421apr2018h.pcr
@18:48:28.60 Building PCR Slug from ASV with MM2 mix, PP4 primer
@18:48:28.61 Priming with carrier
@18:48:28.62 ATV.dial ARV
@18:48:29.28 ARV.dial :carrier
@18:48:29.70 AS.seek 0.5ml
@18:48:55.28 AFV.select :carrierSolution
@18:48:55.38 delay 2 seconds
@18:48:57.41 AS.reconfigure ASconfig
@18:48:57.45 AS.seek 1ml
@18:49:26.45 delay 1 second
@18:49:27.47 AFV.select :holdingCoil
@18:49:27.57 delay 1 second
@18:49:28.59 ATV.dial :waste
@18:49:30.35 AS.reconfigure ASnorm
@18:49:30.40 AS.seek 0ml
@18:50:21.09 AFV.select :carrierSolution
@18:50:21.19 delay 2 seconds
@18:50:23.22 AS.reconfigure ASconfig
@18:50:23.26 AS.seek 0.5ml
@18:50:52.31 delay 1 second
@18:50:53.33 AFV.select :holdingCoil
@18:50:53.43 Building Slug
@18:50:53.44 ATV.dial :ARV
@18:50:55.18 delay 2 seconds
@18:50:57.20 ARV.dial :air1
@18:50:57.59 delay 2 seconds
@18:50:59.66 AS.reconfigure ASslow2
@18:51:00.10 AS.seek 0.506ml
@18:51:06.31 delay 2 seconds
@18:51:08.33 ARV.dial :MM2
@18:51:09.90 delay 2 seconds
@18:51:11.92 Adding MM2
@18:51:11.98 AS.seek 0.524ml
@18:51:30.40 delay 25 seconds
@18:51:55.42 ARV.dial :PP4
@18:51:55.82 Adding PP4
@18:51:55.83 delay 2 seconds
@18:51:57.90 AS.seek 0.53ml
@18:52:04.39 delay 10 seconds
@18:52:14.42 ARV.dial :air3
@18:52:14.82 adding ARV air
@18:52:14.83 delay 2 seconds
@18:52:16.90 AS.seek 0.536ml
@18:52:23.45 delay 5 seconds
@18:52:28.47 ARV.dial :carrier
@18:52:29.76 delay 7 seconds
@18:52:36.84 AS.seek 0.594ml
@18:53:35.64 delay 5 seconds
@18:53:40.66 ATV.dial :ASV
@18:53:41.07 delay 2 seconds
@18:53:43.14 AS.seek 0.6ml
@18:53:49.45 delay 4 seconds
@18:53:53.47 ATV.dial ARV
@18:53:54.03 delay 2 seconds
@18:53:56.05 ARV.dial :carrier
@18:53:56.06 delay 2 seconds
@18:53:58.13 AS.seek 0.65ml
@18:54:49.67 delay 4 seconds
@18:54:53.69 ATV.dial PCR
@18:54:54.32 ASV.dial :waste1
@18:54:54.33 ARV.dial :air1
@18:54:54.72 Positioning Slug by 0.324ml
@18:54:54.79 AS.seek 0.326ml
@19:00:21.09 delay 5 seconds
@19:00:26.11 ATV.dial ARV
@19:00:26.92 ASV.dial :PCRlock
@19:00:27.31 delay 5 seconds
@19:00:32.33 Running AARPP4 Profile58CIQ2 thermocycling
@19:00:32.34 <PCR> PCR.program Profile58CIQ2
@19:00:32.44 Profile58CIQ2 should complete in < 1:36:15
@19:00:32.45 PCR.run 2:05:18.75
@20:21:58.45 PCR.off
@20:21:58.99 <MFBPCR> Running AARPP4 MeltProfile58 thermocycling
@20:21:59.00 <PCR> PCR.program MeltProfile58
@20:21:59.09 MeltProfile58 should complete in < 12:33
@20:21:59.10 PCR.run 20:41.25
@20:30:01.61 PCR.off
@20:30:02.54 <MFBPCR> PCR for AARPP4 completed -- closed /var/log/esp/AARPP421apr2018h.pcr
@20:30:02.60 Cleaning PCR
@20:30:02.61 AFV.select :carrierSolution
@20:30:02.71 delay 2 seconds
@20:30:04.74 AS.reconfigure ASconfig
@20:30:04.78 AS.seek 0.5ml
@20:30:15.07 delay 1 second
@20:30:16.09 AFV.select :holdingCoil
@20:30:16.19 ASV.dial :waste1
@20:30:16.59 ATV.dial :air3
@20:30:17.93 AS.reconfigure ASslow1
@20:30:17.98 AS.seek 0.51ml
@20:30:20.36 delay 2 seconds
@20:30:22.38 ATV.dial :bleach
@20:30:23.01 AS.seek 0.81ml
@20:31:23.60 delay 3 seconds
@20:31:26.62 ATV.dial :air3
@20:31:27.20 delay 3 seconds
@20:31:30.27 AS.seek 0.835ml
@20:31:35.55 delay 3 seconds
@20:31:38.57 ATV.dial :bleach
@20:31:39.20 AS.seek 0.82ml
@20:31:42.46 delay 3 seconds
@20:31:45.48 ARV.dial :waste
@20:31:46.10 delay 3 seconds
@20:31:49.12 ATV.dial :ARV
@20:31:50.04 AS.reconfigure ASconfig
@20:31:50.09 AS.seek 0.47ml
@20:32:10.87 AFV.select :carrierSolution
@20:32:10.97 delay 2 seconds
@20:32:13.00 AS.seek 1ml
@20:32:43.77 delay 1 second
@20:32:44.79 AFV.select :holdingCoil
@20:32:44.89 delay 1 second
@20:32:45.92 AS.reconfigure ASfast
@20:32:45.96 AS.seek 0ml
@20:33:13.29 AFV.select :carrierSolution
@20:33:13.39 delay 2 seconds
@20:33:15.42 AS.reconfigure ASconfig
@20:33:15.46 AS.seek 1ml
@20:34:13.18 delay 1 second
@20:34:14.20 AFV.select :holdingCoil
@20:34:14.30 delay 1 second
@20:34:15.33 AS.reconfigure ASfast
@20:34:15.37 AS.seek 0ml
@20:34:42.70 AFV.select :carrierSolution
@20:34:42.80 delay 2 seconds
@20:34:44.83 AS.reconfigure ASconfig
@20:34:44.87 AS.seek 1ml
@20:35:42.65 delay 1 second
@20:35:43.67 AFV.select :holdingCoil
@20:35:43.77 delay 1 second
@20:35:44.80 AS.reconfigure ASfast
@20:35:44.84 AS.seek 0ml
@20:36:12.15 ARV.dial between carrier and air1
@20:36:12.66 ATV.dial :air3
@20:36:14.02 AS.reconfigure ASslow1
@20:36:14.06 AS.seek 0.01ml
@20:36:16.32 delay 2 seconds
@20:36:18.34 ATV.dial :bleach
@20:36:18.97 AS.seek 0.81ml
@20:39:00.10 delay 3 seconds
@20:39:03.12 ATV.dial :air3
@20:39:03.70 delay 3 seconds
@20:39:06.77 AS.seek 0.835ml
@20:39:12.17 delay 3 seconds
@20:39:15.19 ATV.dial :bleach
@20:39:15.82 AS.seek 0.82ml
@20:39:19.10 delay 3 seconds
@20:39:22.12 ATV.dial PCR
@20:39:22.54 AS.reconfigure ASconfig
@20:39:22.59 AS.seek 0ml
@20:40:09.99 PCR.program :bleachProfile
@20:40:10.05 PCR program should complete in < 2:45
@20:40:10.06 PCR.run 10:00
@20:43:05.83 PCR.off
@20:43:05.86 AFV.select :carrierSolution
@20:43:05.96 delay 2 seconds
@20:43:07.99 AS.seek 1ml
@20:44:05.71 delay 1 second
@20:44:06.73 AFV.select :holdingCoil
@20:44:06.83 delay 1 second
@20:44:07.86 AS.reconfigure ASfast
@20:44:07.90 AS.seek 0ml
@20:44:35.23 delay 3 seconds
@20:44:38.25 AFV.select :carrierSolution
@20:44:38.35 delay 2 seconds
@20:44:40.37 AS.reconfigure ASconfig
@20:44:40.43 AS.seek 1ml
@20:45:38.16 delay 1 second
@20:45:39.18 AFV.select :holdingCoil
@20:45:39.28 delay 1 second
@20:45:40.31 AS.reconfigure ASfast
@20:45:40.76 AS.seek 0ml
@20:46:08.07 delay 3 seconds
@20:46:11.09 AFV.select :carrierSolution
@20:46:11.19 delay 2 seconds
@20:46:13.22 AS.reconfigure ASconfig
@20:46:13.27 AS.seek 1ml
@20:47:11.01 delay 1 second
@20:47:12.03 AFV.select :holdingCoil
@20:47:12.13 delay 1 second
@20:47:13.16 AS.reconfigure ASfast
@20:47:13.20 AS.seek 0ml
@20:47:40.54 delay 3 seconds
@20:47:43.56 AFV.select :carrierSolution
@20:47:43.66 delay 2 seconds
@20:47:45.69 AS.reconfigure ASconfig
@20:47:45.74 AS.seek 1ml
@20:48:43.51 delay 1 second
@20:48:44.53 AFV.select :holdingCoil
@20:48:44.63 delay 1 second
@20:48:45.66 AS.reconfigure ASfast
@20:48:45.70 AS.seek 0ml
@20:49:13.04 delay 3 seconds
@20:49:16.06 AFV.select :carrierSolution
@20:49:16.16 delay 2 seconds
@20:49:18.19 AS.reconfigure ASconfig
@20:49:18.24 AS.seek 1ml
@20:50:15.98 delay 1 second
@20:50:17.00 AFV.select :holdingCoil
@20:50:17.10 delay 1 second
@20:50:18.13 AS.reconfigure ASnorm
@20:50:18.17 AS.seek 0ml
@20:51:08.89 Setting up PCR for AALPP3 -- writing /var/log/esp/AALPP321apr2020h.pcr
@20:51:08.91 Building PCR Slug from ASV with MM2 mix, PP3 primer
@20:51:08.92 Priming with carrier
@20:51:08.93 ATV.dial ARV
@20:51:09.57 ARV.dial :carrier
@20:51:09.91 AS.seek 0.5ml
@20:51:35.48 AFV.select :carrierSolution
@20:51:35.58 delay 2 seconds
@20:51:37.61 AS.reconfigure ASconfig
@20:51:37.65 AS.seek 1ml
@20:52:06.65 delay 1 second
@20:52:07.67 AFV.select :holdingCoil
@20:52:07.77 delay 1 second
@20:52:08.79 ATV.dial :waste
@20:52:10.57 AS.reconfigure ASnorm
@20:52:10.62 AS.seek 0ml
@20:53:01.30 AFV.select :carrierSolution
@20:53:01.40 delay 2 seconds
@20:53:03.43 AS.reconfigure ASconfig
@20:53:03.47 AS.seek 0.5ml
@20:53:32.52 delay 1 second
@20:53:33.54 AFV.select :holdingCoil
@20:53:33.64 Building Slug
@20:53:33.65 ATV.dial :ARV
@20:53:35.40 delay 2 seconds
@20:53:37.42 ARV.dial :air1
@20:53:37.81 delay 2 seconds
@20:53:39.88 AS.reconfigure ASslow2
@20:53:39.92 AS.seek 0.506ml
@20:53:46.26 delay 2 seconds
@20:53:48.28 ARV.dial :MM2
@20:53:49.84 delay 2 seconds
@20:53:51.86 Adding MM2
@20:53:51.92 AS.seek 0.524ml
@20:54:10.12 delay 25 seconds
@20:54:35.14 ARV.dial :PP3
@20:54:35.52 Adding PP3
@20:54:35.53 delay 2 seconds
@20:54:37.60 AS.seek 0.53ml
@20:54:43.98 delay 10 seconds
@20:54:54.01 ARV.dial :air2
@20:54:54.41 adding ARV air
@20:54:54.42 delay 2 seconds
@20:54:56.49 AS.seek 0.536ml
@20:55:02.96 delay 5 seconds
@20:55:07.98 ARV.dial :carrier
@20:55:09.32 delay 7 seconds
@20:55:16.40 AS.seek 0.594ml
@20:56:14.90 delay 5 seconds
@20:56:19.92 ATV.dial :ASV
@20:56:20.34 delay 2 seconds
@20:56:22.41 AS.seek 0.6ml
@20:56:28.79 delay 4 seconds
@20:56:32.81 ATV.dial ARV
@20:56:33.38 delay 2 seconds
@20:56:35.40 ARV.dial :carrier
@20:56:35.41 delay 2 seconds
@20:56:37.48 AS.seek 0.65ml
@20:57:27.87 delay 4 seconds
@20:57:31.89 ATV.dial PCR
@20:57:32.52 ASV.dial :waste1
@20:57:32.53 ARV.dial :air1
@20:57:32.92 Positioning Slug by 0.324ml
@20:57:32.99 AS.seek 0.326ml
@21:02:58.94 delay 5 seconds
@21:03:03.96 ATV.dial ARV
@21:03:04.88 ASV.dial :PCRlock
@21:03:05.27 delay 5 seconds
@21:03:10.29 Running AALPP3 Profile58CIQ2 thermocycling
@21:03:10.31 <PCR> PCR.program Profile58CIQ2
@21:03:10.40 Profile58CIQ2 should complete in < 1:36:15
@21:03:10.41 PCR.run 2:05:18.75
@22:24:28.99 PCR.off
@22:24:29.52 <MFBPCR> Running AALPP3 MeltProfile58 thermocycling
@22:24:29.54 <PCR> PCR.program MeltProfile58
@22:24:29.62 MeltProfile58 should complete in < 12:33
@22:24:29.63 PCR.run 20:41.25
@22:32:26.71 PCR.off
@22:32:27.65 <MFBPCR> PCR for AALPP3 completed -- closed /var/log/esp/AALPP321apr2020h.pcr
@22:32:27.71 Cleaning PCR
@22:32:27.72 AFV.select :carrierSolution
@22:32:27.82 delay 2 seconds
@22:32:29.85 AS.reconfigure ASconfig
@22:32:29.89 AS.seek 0.5ml
@22:32:40.22 delay 1 second
@22:32:41.24 AFV.select :holdingCoil
@22:32:41.34 ASV.dial :waste1
@22:32:41.72 ATV.dial :air3
@22:32:43.07 AS.reconfigure ASslow1
@22:32:43.12 AS.seek 0.51ml
@22:32:45.41 delay 2 seconds
@22:32:47.43 ATV.dial :bleach
@22:32:48.08 AS.seek 0.81ml
@22:33:48.66 delay 3 seconds
@22:33:51.68 ATV.dial :air3
@22:33:52.27 delay 3 seconds
@22:33:55.34 AS.seek 0.835ml
@22:34:00.60 delay 3 seconds
@22:34:03.62 ATV.dial :bleach
@22:34:04.27 AS.seek 0.82ml
@22:34:07.52 delay 3 seconds
@22:34:10.54 ARV.dial :waste
@22:34:11.16 delay 3 seconds
@22:34:14.18 ATV.dial :ARV
@22:34:15.09 AS.reconfigure ASconfig
@22:34:15.14 AS.seek 0.47ml
@22:34:35.52 AFV.select :carrierSolution
@22:34:35.62 delay 2 seconds
@22:34:37.65 AS.seek 1ml
@22:35:08.36 delay 1 second
@22:35:09.38 AFV.select :holdingCoil
@22:35:09.48 delay 1 second
@22:35:10.51 AS.reconfigure ASfast
@22:35:10.55 AS.seek 0ml
@22:35:37.87 AFV.select :carrierSolution
@22:35:37.97 delay 2 seconds
@22:35:39.99 AS.reconfigure ASconfig
@22:35:40.04 AS.seek 1ml
@22:36:37.82 delay 1 second
@22:36:38.84 AFV.select :holdingCoil
@22:36:38.94 delay 1 second
@22:36:39.97 AS.reconfigure ASfast
@22:36:40.01 AS.seek 0ml
@22:37:07.33 AFV.select :carrierSolution
@22:37:07.43 delay 2 seconds
@22:37:09.45 AS.reconfigure ASconfig
@22:37:09.50 AS.seek 1ml
@22:38:07.24 delay 1 second
@22:38:08.26 AFV.select :holdingCoil
@22:38:08.36 delay 1 second
@22:38:09.39 AS.reconfigure ASfast
@22:38:09.43 AS.seek 0ml
@22:38:36.76 ARV.dial between carrier and air1
@22:38:37.27 ATV.dial :air3
@22:38:38.60 AS.reconfigure ASslow1
@22:38:38.65 AS.seek 0.01ml
@22:38:41.07 delay 2 seconds
@22:38:43.09 ATV.dial :bleach
@22:38:43.73 AS.seek 0.81ml
@22:41:24.90 delay 3 seconds
@22:41:27.92 ATV.dial :air3
@22:41:28.49 delay 3 seconds
@22:41:31.56 AS.seek 0.835ml
@22:41:36.86 delay 3 seconds
@22:41:39.88 ATV.dial :bleach
@22:41:40.52 AS.seek 0.82ml
@22:41:43.83 delay 3 seconds
@22:41:46.85 ATV.dial PCR
@22:41:47.27 AS.reconfigure ASconfig
@22:41:47.32 AS.seek 0ml
@22:42:34.72 PCR.program :bleachProfile
@22:42:34.78 PCR program should complete in < 2:45
@22:42:34.79 PCR.run 10:00
@22:45:30.97 PCR.off
@22:45:31.00 AFV.select :carrierSolution
@22:45:31.10 delay 2 seconds
@22:45:33.13 AS.seek 1ml
@22:46:30.94 delay 1 second
@22:46:31.96 AFV.select :holdingCoil
@22:46:32.06 delay 1 second
@22:46:33.09 AS.reconfigure ASfast
@22:46:33.13 AS.seek 0ml
@22:47:00.47 delay 3 seconds
@22:47:03.49 AFV.select :carrierSolution
@22:47:03.59 delay 2 seconds
@22:47:05.62 AS.reconfigure ASconfig
@22:47:05.67 AS.seek 1ml
@22:48:03.41 delay 1 second
@22:48:04.43 AFV.select :holdingCoil
@22:48:04.53 delay 1 second
@22:48:05.56 AS.reconfigure ASfast
@22:48:05.60 AS.seek 0ml
@22:48:32.94 delay 3 seconds
@22:48:35.96 AFV.select :carrierSolution
@22:48:36.06 delay 2 seconds
@22:48:38.08 AS.reconfigure ASconfig
@22:48:38.14 AS.seek 1ml
@22:49:35.90 delay 1 second
@22:49:36.92 AFV.select :holdingCoil
@22:49:37.02 delay 1 second
@22:49:38.05 AS.reconfigure ASfast
@22:49:38.09 AS.seek 0ml
@22:50:05.43 delay 3 seconds
@22:50:08.45 AFV.select :carrierSolution
@22:50:08.55 delay 2 seconds
@22:50:10.57 AS.reconfigure ASconfig
@22:50:10.62 AS.seek 1ml
@22:51:08.36 delay 1 second
@22:51:09.38 AFV.select :holdingCoil
@22:51:09.48 delay 1 second
@22:51:10.51 AS.reconfigure ASfast
@22:51:10.55 AS.seek 0ml
@22:51:37.90 delay 3 seconds
@22:51:40.92 AFV.select :carrierSolution
@22:51:41.02 delay 2 seconds
@22:51:43.04 AS.reconfigure ASconfig
@22:51:43.10 AS.seek 1ml
@22:52:40.83 delay 1 second
@22:52:41.85 AFV.select :holdingCoil
@22:52:41.95 delay 1 second
@22:52:42.98 AS.reconfigure ASnorm
@22:52:43.02 AS.seek 0ml
@22:53:33.70 Setting up PCR for ACOPP5 -- writing /var/log/esp/ACOPP521apr2022h.pcr
@22:53:33.72 Building PCR Slug from ASV with MM3 mix, PP5 primer
@22:53:33.73 Priming with carrier
@22:53:33.74 ATV.dial ARV
@22:53:34.36 ARV.dial :carrier
@22:53:34.71 AS.seek 0.5ml
@22:54:00.29 AFV.select :carrierSolution
@22:54:00.39 delay 2 seconds
@22:54:02.42 AS.reconfigure ASconfig
@22:54:02.46 AS.seek 1ml
@22:54:31.49 delay 1 second
@22:54:32.51 AFV.select :holdingCoil
@22:54:32.61 delay 1 second
@22:54:33.63 ATV.dial :waste
@22:54:35.40 AS.reconfigure ASnorm
@22:54:35.45 AS.seek 0ml
@22:55:26.10 AFV.select :carrierSolution
@22:55:26.20 delay 2 seconds
@22:55:28.23 AS.reconfigure ASconfig
@22:55:28.27 AS.seek 0.5ml
@22:55:57.32 delay 1 second
@22:55:58.34 AFV.select :holdingCoil
@22:55:58.44 Building Slug
@22:55:58.45 ATV.dial :ARV
@22:56:00.30 delay 2 seconds
@22:56:02.32 ARV.dial :air1
@22:56:02.72 delay 2 seconds
@22:56:04.79 AS.reconfigure ASslow2
@22:56:04.83 AS.seek 0.506ml
@22:56:11.05 delay 2 seconds
@22:56:13.07 ARV.dial :MM3
@22:56:14.16 delay 2 seconds
@22:56:16.18 Adding MM3
@22:56:16.25 AS.seek 0.524ml
@22:56:34.63 delay 25 seconds
@22:56:59.65 ARV.dial :PP5
@22:57:00.05 Adding PP5
@22:57:00.06 delay 2 seconds
@22:57:02.13 AS.seek 0.53ml
@22:57:08.68 delay 10 seconds
@22:57:18.71 ARV.dial :air3
@22:57:19.10 adding ARV air
@22:57:19.11 delay 2 seconds
@22:57:21.18 AS.seek 0.536ml
@22:57:27.60 delay 5 seconds
@22:57:32.62 ARV.dial :carrier
@22:57:34.35 delay 7 seconds
@22:57:41.43 AS.seek 0.594ml
@22:58:40.07 delay 5 seconds
@22:58:45.09 ATV.dial :ASV
@22:58:45.51 delay 2 seconds
@22:58:47.58 AS.seek 0.6ml
@22:58:53.93 delay 4 seconds
@22:58:57.95 ATV.dial ARV
@22:58:58.52 delay 2 seconds
@22:59:00.54 ARV.dial :carrier
@22:59:00.55 delay 2 seconds
@22:59:02.62 AS.seek 0.65ml
@22:59:53.43 delay 4 seconds
@22:59:57.45 ATV.dial PCR
@22:59:58.08 ASV.dial :waste1
@22:59:58.09 ARV.dial :air1
@22:59:58.51 Positioning Slug by 0.324ml
@22:59:58.58 AS.seek 0.326ml
@23:05:24.94 delay 5 seconds
@23:05:29.96 ATV.dial ARV
@23:05:30.88 ASV.dial :PCRlock
@23:05:31.27 delay 5 seconds
@23:05:36.29 Running ACOPP5 ProfileIPc thermocycling
@23:05:36.31 <PCR> PCR.program ProfileIPc
@23:05:36.41 ProfileIPc should complete in < 1:26:35
@23:05:36.42 PCR.run 1:53:13.75
@00:14:37.79CEST21-Apr-21 PCR.off
@00:14:38.33 <MFBPCR> PCR for ACOPP5 completed -- closed /var/log/esp/ACOPP521apr2022h.pcr
@00:14:38.38 Cleaning PCR
@00:14:38.39 AFV.select :carrierSolution
@00:14:38.49 delay 2 seconds
@00:14:40.52 AS.reconfigure ASconfig
@00:14:40.56 AS.seek 0.5ml
@00:14:50.86 delay 1 second
@00:14:51.88 AFV.select :holdingCoil
@00:14:51.98 ASV.dial :waste1
@00:14:52.38 ATV.dial :air3
@00:14:53.73 AS.reconfigure ASslow1
@00:14:53.78 AS.seek 0.51ml
@00:14:56.06 delay 2 seconds
@00:14:58.08 ATV.dial :bleach
@00:14:58.73 AS.seek 0.81ml
@00:15:59.44 delay 3 seconds
@00:16:02.46 ATV.dial :air3
@00:16:03.04 delay 3 seconds
@00:16:06.19 AS.seek 0.835ml
@00:16:11.50 delay 3 seconds
@00:16:14.52 ATV.dial :bleach
@00:16:15.15 AS.seek 0.82ml
@00:16:18.46 delay 3 seconds
@00:16:21.48 ARV.dial :waste
@00:16:22.16 delay 3 seconds
@00:16:25.18 ATV.dial :ARV
@00:16:26.09 AS.reconfigure ASconfig
@00:16:26.14 AS.seek 0.47ml
@00:16:46.54 AFV.select :carrierSolution
@00:16:46.64 delay 2 seconds
@00:16:48.67 AS.seek 1ml
@00:17:19.44 delay 1 second
@00:17:20.46 AFV.select :holdingCoil
@00:17:20.56 delay 1 second
@00:17:21.59 AS.reconfigure ASfast
@00:17:21.63 AS.seek 0ml
@00:17:48.96 AFV.select :carrierSolution
@00:17:49.06 delay 2 seconds
@00:17:51.08 AS.reconfigure ASconfig
@00:17:51.13 AS.seek 1ml
@00:18:48.91 delay 1 second
@00:18:49.93 AFV.select :holdingCoil
@00:18:50.03 delay 1 second
@00:18:51.06 AS.reconfigure ASfast
@00:18:51.10 AS.seek 0ml
@00:19:18.43 AFV.select :carrierSolution
@00:19:18.53 delay 2 seconds
@00:19:20.55 AS.reconfigure ASconfig
@00:19:20.60 AS.seek 1ml
@00:20:18.35 delay 1 second
@00:20:19.37 AFV.select :holdingCoil
@00:20:19.47 delay 1 second
@00:20:20.50 AS.reconfigure ASfast
@00:20:20.54 AS.seek 0ml
@00:20:47.85 ARV.dial between carrier and air1
@00:20:48.35 ATV.dial :air3
@00:20:49.68 AS.reconfigure ASslow1
@00:20:49.73 AS.seek 0.01ml
@00:20:52.10 delay 2 seconds
@00:20:54.12 ATV.dial :bleach
@00:20:54.74 AS.seek 0.81ml
@00:23:36.00 delay 3 seconds
@00:23:39.02 ATV.dial :air3
@00:23:39.60 delay 3 seconds
@00:23:42.67 AS.seek 0.835ml
@00:23:48.03 delay 3 seconds
@00:23:51.05 ATV.dial :bleach
@00:23:51.68 AS.seek 0.82ml
@00:23:55.05 delay 3 seconds
@00:23:58.07 ATV.dial PCR
@00:23:58.49 AS.reconfigure ASconfig
@00:23:58.54 AS.seek 0ml
@00:24:45.96 PCR.program :bleachProfile
@00:24:46.01 PCR program should complete in < 2:45
@00:24:46.02 PCR.run 10:00
@00:27:42.21 PCR.off
@00:27:42.24 AFV.select :carrierSolution
@00:27:42.34 delay 2 seconds
@00:27:44.37 AS.seek 1ml
@00:28:42.14 delay 1 second
@00:28:43.16 AFV.select :holdingCoil
@00:28:43.26 delay 1 second
@00:28:44.29 AS.reconfigure ASfast
@00:28:44.33 AS.seek 0ml
@00:29:11.68 delay 3 seconds
@00:29:14.70 AFV.select :carrierSolution
@00:29:14.80 delay 2 seconds
@00:29:16.83 AS.reconfigure ASconfig
@00:29:16.88 AS.seek 1ml
@00:30:14.61 delay 1 second
@00:30:15.63 AFV.select :holdingCoil
@00:30:15.73 delay 1 second
@00:30:16.76 AS.reconfigure ASfast
@00:30:16.80 AS.seek 0ml
@00:30:44.13 delay 3 seconds
@00:30:47.15 AFV.select :carrierSolution
@00:30:47.25 delay 2 seconds
@00:30:49.27 AS.reconfigure ASconfig
@00:30:49.32 AS.seek 1ml
@00:31:47.07 delay 1 second
@00:31:48.09 AFV.select :holdingCoil
@00:31:48.19 delay 1 second
@00:31:49.22 AS.reconfigure ASfast
@00:31:49.26 AS.seek 0ml
@00:32:16.60 delay 3 seconds
@00:32:19.62 AFV.select :carrierSolution
@00:32:19.72 delay 2 seconds
@00:32:21.75 AS.reconfigure ASconfig
@00:32:21.80 AS.seek 1ml
@00:33:19.57 delay 1 second
@00:33:20.59 AFV.select :holdingCoil
@00:33:20.69 delay 1 second
@00:33:21.72 AS.reconfigure ASfast
@00:33:21.76 AS.seek 0ml
@00:33:49.10 delay 3 seconds
@00:33:52.12 AFV.select :carrierSolution
@00:33:52.22 delay 2 seconds
@00:33:54.24 AS.reconfigure ASconfig
@00:33:54.29 AS.seek 1ml
@00:34:52.04 delay 1 second
@00:34:53.06 AFV.select :holdingCoil
@00:34:53.16 delay 1 second
@00:34:54.19 AS.reconfigure ASnorm
@00:34:54.23 AS.seek 0ml
@00:35:44.93 Setting up PCR for AIPc -- writing /var/log/esp/AIPc21apr2100h.pcr
@00:35:44.95 Building PCR Slug from ASV with MM3 mix, PP6 primer
@00:35:44.96 Priming with carrier
@00:35:44.97 ATV.dial ARV
@00:35:45.60 ARV.dial :carrier
@00:35:45.92 AS.seek 0.5ml
@00:36:11.46 AFV.select :carrierSolution
@00:36:11.56 delay 2 seconds
@00:36:13.59 AS.reconfigure ASconfig
@00:36:13.63 AS.seek 1ml
@00:36:42.64 delay 1 second
@00:36:43.66 AFV.select :holdingCoil
@00:36:43.76 delay 1 second
@00:36:44.78 ATV.dial :waste
@00:36:46.54 AS.reconfigure ASnorm
@00:36:46.59 AS.seek 0ml
@00:37:37.25 AFV.select :carrierSolution
@00:37:37.35 delay 2 seconds
@00:37:39.38 AS.reconfigure ASconfig
@00:37:39.42 AS.seek 0.5ml
@00:38:08.49 delay 1 second
@00:38:09.51 AFV.select :holdingCoil
@00:38:09.61 Building Slug
@00:38:09.62 ATV.dial :ARV
@00:38:11.36 delay 2 seconds
@00:38:13.38 ARV.dial :air1
@00:38:13.78 delay 2 seconds
@00:38:15.85 AS.reconfigure ASslow2
@00:38:15.89 AS.seek 0.506ml
@00:38:22.35 delay 2 seconds
@00:38:24.37 ARV.dial :MM3
@00:38:25.46 delay 2 seconds
@00:38:27.89 Adding MM3
@00:38:27.95 AS.seek 0.524ml
@00:38:46.24 delay 25 seconds
@00:39:11.26 ARV.dial :PP6
@00:39:11.64 Adding PP6
@00:39:11.65 delay 2 seconds
@00:39:13.72 AS.seek 0.53ml
@00:39:20.11 delay 10 seconds
@00:39:30.14 ARV.dial :air1
@00:39:31.00 adding ARV air
@00:39:31.01 delay 2 seconds
@00:39:33.08 AS.seek 0.536ml
@00:39:39.52 delay 5 seconds
@00:39:44.54 ARV.dial :carrier
@00:39:44.94 delay 7 seconds
@00:39:52.02 AS.seek 0.594ml
@00:40:50.82 delay 5 seconds
@00:40:55.84 ATV.dial :ASV
@00:40:56.25 delay 2 seconds
@00:40:58.32 AS.seek 0.6ml
@00:41:04.88 delay 4 seconds
@00:41:08.90 ATV.dial ARV
@00:41:09.47 delay 2 seconds
@00:41:11.49 ARV.dial :carrier
@00:41:11.50 delay 2 seconds
@00:41:13.57 AS.seek 0.65ml
@00:42:04.07 delay 4 seconds
@00:42:08.09 ATV.dial PCR
@00:42:08.72 ASV.dial :waste1
@00:42:08.73 ARV.dial :air1
@00:42:09.31 Positioning Slug by 0.324ml
@00:42:09.38 AS.seek 0.326ml
@00:47:35.61 delay 5 seconds
@00:47:40.63 ATV.dial ARV
@00:47:41.44 ASV.dial :PCRlock
@00:47:41.83 delay 5 seconds
@00:47:46.85 Running AIPc ProfileIPc thermocycling
@00:47:46.87 <PCR> PCR.program ProfileIPc
@00:47:46.96 ProfileIPc should complete in < 1:26:35
@00:47:46.97 PCR.run 1:53:13.75
@01:56:42.49 PCR.off
@01:56:43.03 <MFBPCR> PCR for AIPc completed -- closed /var/log/esp/AIPc21apr2100h.pcr
@01:56:43.04 Full MFB Decontamination
@01:56:43.05 Decon extraction column and decon extSrc
@01:56:43.07 SPE.hold 2:46:40,55C
@01:56:43.11 ASV.dial :SPEdelivery
@01:56:43.78 delay 20 seconds
@01:57:03.81 AFV.select :carrierSolution
@01:57:03.91 delay 2 seconds
@01:57:05.94 AS.reconfigure ASconfig
@01:57:05.98 AS.seek 0.5ml
@01:57:16.28 delay 1 second
@01:57:17.30 AFV.select :holdingCoil
@01:57:17.40 ATV.dial :air1
@01:57:19.66 AS.reconfigure ASslow1
@01:57:19.71 AS.seek 0.52ml
@01:57:24.14 ATV.dial :ASV
@01:57:25.89 ASV.dial :SPErecovery
@01:57:26.58 AS.seek 0.62ml
@01:57:46.99 ATV.dial :waste
@01:57:49.18 AS.reconfigure ASnorm
@01:57:49.23 AS.seek 0ml
@01:58:20.78 deconEluateRecoveryLine extSrc
@01:58:20.79 ATV.dial :waste
@01:58:20.81 AS.seek 0ml
@01:58:20.86 ATV.dial :air3
@01:58:21.63 AS.reconfigure ASslow1
@01:58:21.67 AS.seek 0.01ml
@01:58:24.08 delay 2 seconds
@01:58:26.10 ATV.dial :bleach
@01:58:26.59 AS.seek 0.31ml
@01:59:27.30 delay 3 seconds
@01:59:30.32 ATV.dial :air3
@01:59:30.92 delay 3 seconds
@01:59:33.99 AS.seek 0.335ml
@01:59:39.39 delay 3 seconds
@01:59:42.41 ATV.dial :bleach
@01:59:43.08 AS.seek 0.32ml
@01:59:46.38 delay 3 seconds
@01:59:49.40 ATV.dial :extSrc
@01:59:50.05 AS.seek 0ml
@02:00:54.67 AFV.select :carrierSolution
@02:00:54.77 delay 2 seconds
@02:00:56.80 AS.reconfigure ASconfig
@02:00:56.84 AS.seek 0.5ml
@02:01:25.88 delay 1 second
@02:01:26.90 AFV.select :holdingCoil
@02:01:27.00 ATV.dial :extSrc
@02:01:27.02 AS.reconfigure ASslow1
@02:01:27.06 AS.seek 0ml
@02:03:07.89 AFV.select :carrierSolution
@02:03:07.99 delay 2 seconds
@02:03:10.02 AS.reconfigure ASconfig
@02:03:10.06 AS.seek 0.5ml
@02:03:39.08 delay 1 second
@02:03:40.10 AFV.select :holdingCoil
@02:03:40.20 ATV.dial :extSrc
@02:03:40.22 AS.reconfigure ASslow1
@02:03:40.26 AS.seek 0ml
@02:05:21.10 AFV.select :carrierSolution
@02:05:21.20 delay 2 seconds
@02:05:23.23 AS.reconfigure ASconfig
@02:05:23.27 AS.seek 0.5ml
@02:05:52.30 delay 1 second
@02:05:53.32 AFV.select :holdingCoil
@02:05:53.42 ATV.dial :extSrc
@02:05:53.44 AS.reconfigure ASslow1
@02:05:53.48 AS.seek 0ml
@02:07:34.28 AFV.select :carrierSolution
@02:07:34.38 delay 2 seconds
@02:07:36.41 AS.reconfigure ASconfig
@02:07:36.45 AS.seek 0.5ml
@02:08:05.49 delay 1 second
@02:08:06.92 AFV.select :holdingCoil
@02:08:07.02 ATV.dial :extSrc
@02:08:07.04 AS.reconfigure ASslow1
@02:08:07.08 AS.seek 0ml
@02:09:47.91 ATV.dial :air2
@02:09:49.30 AS.reconfigure ASslow0
@02:09:49.34 AS.seek 0.5ml
@02:10:39.89 ATV.dial :extSrc
@02:10:41.44 AS.seek 0.02ml
@02:11:30.02 delay 1 second
@02:11:31.04 ATV.dial :waste
@02:11:32.38 AS.reconfigure ASconfig
@02:11:32.43 AS.seek 0ml
@02:11:33.86 AFV.select :holdingCoil
@02:11:33.87 ATV.dial :waste
@02:11:33.89 AS.seek 0ml
@02:11:33.94 AFV.select :carrierSolution
@02:11:34.04 delay 2 seconds
@02:11:36.07 AS.seek 0.5ml
@02:12:05.08 delay 1 second
@02:12:06.10 AFV.select :holdingCoil
@02:12:06.20 ATV.dial :air3
@02:12:07.08 AS.reconfigure ASslow1
@02:12:07.12 AS.seek 0.52ml
@02:12:11.44 ATV.dial :air3
@02:12:11.51 AS.seek 0.53ml
@02:12:13.91 delay 2 seconds
@02:12:15.93 ATV.dial :bleach
@02:12:16.41 AS.seek 0.71ml
@02:12:52.88 delay 3 seconds
@02:12:55.90 ATV.dial :air3
@02:12:56.49 delay 3 seconds
@02:12:59.56 AS.seek 0.735ml
@02:13:04.86 delay 3 seconds
@02:13:07.88 ATV.dial :bleach
@02:13:08.53 AS.seek 0.72ml
@02:13:11.83 delay 3 seconds
@02:13:14.85 ATV.dial ASV
@02:13:15.94 ASV.dial :SPErecovery
@02:13:16.01 AS.seek 0.365ml
@02:14:27.64 ATV.dial :waste
@02:14:29.64 AS.reconfigure ASnorm
@02:14:29.69 AS.seek 0ml
@02:14:48.42 ATV.dial :air3
@02:14:49.31 AS.reconfigure ASslow1
@02:14:49.35 AS.seek 0.01ml
@02:14:51.74 delay 2 seconds
@02:14:53.76 ATV.dial :bleach
@02:14:54.23 AS.seek 0.58ml
@02:16:49.24 delay 3 seconds
@02:16:52.26 ATV.dial :air3
@02:16:52.85 delay 3 seconds
@02:16:55.92 AS.seek 0.605ml
@02:17:01.23 delay 3 seconds
@02:17:04.25 ATV.dial :bleach
@02:17:04.91 AS.seek 0.59ml
@02:17:08.19 delay 3 seconds
@02:17:11.21 ATV.dial :ASV
@02:17:12.32 ASV.dial :wasteR
@02:17:12.71 AS.seek 0ml
@02:19:11.63 delay 1:00
@02:20:11.66 ATV.dial ASV
@02:20:11.67 AFV.select :carrierSolution
@02:20:11.77 delay 2 seconds
@02:20:13.79 AS.reconfigure ASconfig
@02:20:13.84 AS.seek 1ml
@02:21:11.61 delay 1 second
@02:21:12.63 AFV.select :holdingCoil
@02:21:12.73 delay 1 second
@02:21:13.75 ASV.dial :wasteR
@02:21:13.77 AS.reconfigure ASslow1
@02:21:13.81 AS.seek 0ml
@02:24:35.19 delay 10 seconds
@02:24:45.22 ATV.dial :air3
@02:24:46.83 AS.seek 0.01ml
@02:24:49.14 delay 2 seconds
@02:24:51.16 ATV.dial :bleach
@02:24:51.81 AS.seek 0.58ml
@02:26:46.71 delay 3 seconds
@02:26:49.76 ATV.dial :air3
@02:26:50.33 delay 3 seconds
@02:26:53.40 AS.seek 0.605ml
@02:26:58.69 delay 3 seconds
@02:27:01.71 ATV.dial :bleach
@02:27:02.34 AS.seek 0.59ml
@02:27:05.66 delay 3 seconds
@02:27:08.68 ATV.dial ASV
@02:27:09.80 ASV.dial :SPEdelivery
@02:27:10.21 AS.seek 0ml
@02:29:09.16 delay 1:00
@02:30:09.19 AFV.select :carrierSolution
@02:30:09.29 delay 2 seconds
@02:30:11.32 AS.reconfigure ASconfig
@02:30:11.36 AS.seek 1ml
@02:31:09.11 delay 1 second
@02:31:10.13 AFV.select :holdingCoil
@02:31:10.23 delay 1 second
@02:31:11.25 ATV.dial ASV
@02:31:11.26 ASV.dial :SPEdelivery
@02:31:11.28 AS.reconfigure ASslow1
@02:31:11.32 AS.seek 0ml
@02:34:32.72 delay 10 seconds
@02:34:42.75 ATV.dial :air2
@02:34:43.63 AS.reconfigure ASslow0
@02:34:43.68 AS.seek 0.8ml
@02:36:04.38 delay 3 seconds
@02:36:07.40 ATV.dial :ASV
@02:36:08.42 ASV.dial :SPEdelivery
@02:36:08.80 AS.reconfigure ASslow1
@02:36:08.91 AS.seek 0.03ml
@02:38:44.16 delay 20 seconds
@02:39:04.19 ATV.dial :waste,via: :air3
@02:39:06.03 AS.reconfigure ASconfig
@02:39:06.08 AS.seek 0ml
@02:39:08.08 SPE.coast
@02:39:08.15 Removing Bleach from Mixing Coil
@02:39:08.16 AFV.select :carrierSolution
@02:39:08.26 delay 2 seconds
@02:39:10.29 AS.seek 0.5ml
@02:39:39.30 delay 1 second
@02:39:40.32 AFV.select :holdingCoil
@02:39:40.42 ATV.dial :ASV
@02:39:42.21 ASV.dial :SPErecovery
@02:39:42.86 AS.reconfigure ASslow1
@02:39:42.90 AS.seek 0.73ml
@02:40:29.44 ASV.dial :wasteD
@02:40:30.11 AS.reconfigure ASnorm
@02:40:30.16 AS.seek 0ml
@02:41:07.25 AFV.select :carrierSolution
@02:41:07.35 delay 2 seconds
@02:41:09.38 AS.reconfigure ASconfig
@02:41:09.42 AS.seek 0.5ml
@02:41:38.44 delay 1 second
@02:41:39.46 AFV.select :holdingCoil
@02:41:39.56 ATV.dial ASV
@02:41:39.58 ASV.dial :SPErecovery
@02:41:40.23 AS.reconfigure ASslow0
@02:41:40.27 AS.seek 0.3ml
@02:42:00.69 ATV.dial :air2
@02:42:01.78 AS.reconfigure ASslow1
@02:42:01.82 AS.seek 0.32ml
@02:42:06.12 ATV.dial ASV
@02:42:07.19 AS.reconfigure ASslow0
@02:42:07.24 AS.seek 0.57ml
@02:42:32.71 ASV.dial :wasteD
@02:42:33.30 AS.reconfigure ASnorm
@02:42:33.35 AS.seek 0ml
@02:43:02.39 AFV.select :carrierSolution
@02:43:02.49 delay 2 seconds
@02:43:04.52 AS.reconfigure ASconfig
@02:43:04.56 AS.seek 0.5ml
@02:43:33.58 delay 1 second
@02:43:34.60 AFV.select :holdingCoil
@02:43:34.70 ATV.dial ASV
@02:43:34.72 ASV.dial :SPErecovery
@02:43:35.37 AS.reconfigure ASslow0
@02:43:35.41 AS.seek 0.3ml
@02:43:55.80 ATV.dial :air2
@02:43:56.88 AS.reconfigure ASslow1
@02:43:56.93 AS.seek 0.32ml
@02:44:01.32 ATV.dial ASV
@02:44:02.40 AS.reconfigure ASslow0
@02:44:02.45 AS.seek 0.57ml
@02:44:27.94 ASV.dial :wasteD
@02:44:28.53 AS.reconfigure ASnorm
@02:44:28.58 AS.seek 0ml
@02:44:57.64 ATV.dial :air2
@02:44:58.66 AS.reconfigure ASslow0
@02:44:58.71 AS.seek 0.3ml
@02:45:29.27 ATV.dial :ASV
@02:45:30.30 ASV.dial :wasteD
@02:45:30.31 AS.reconfigure ASconfig
@02:45:30.36 AS.seek 0.05ml
@02:45:45.03 ATV.dial :waste
@02:45:47.00 AS.seek 0ml
@02:45:50.19 ASV.dial :SPEdelivery
@02:45:51.26 Cleaning PCR
@02:45:51.27 AFV.select :carrierSolution
@02:45:51.37 delay 2 seconds
@02:45:53.40 AS.seek 0.5ml
@02:46:22.44 delay 1 second
@02:46:23.46 AFV.select :holdingCoil
@02:46:23.56 ASV.dial :waste1
@02:46:23.94 ATV.dial :air3
@02:46:24.63 AS.reconfigure ASslow1
@02:46:24.68 AS.seek 0.51ml
@02:46:27.06 delay 2 seconds
@02:46:29.08 ATV.dial :bleach
@02:46:29.55 AS.seek 0.81ml
@02:47:30.19 delay 3 seconds
@02:47:33.21 ATV.dial :air3
@02:47:33.78 delay 3 seconds
@02:47:36.85 AS.seek 0.835ml
@02:47:42.12 delay 3 seconds
@02:47:45.14 ATV.dial :bleach
@02:47:45.78 AS.seek 0.82ml
@02:47:49.08 delay 3 seconds
@02:47:52.10 ARV.dial :waste
@02:47:52.72 delay 3 seconds
@02:47:55.74 ATV.dial :ARV
@02:47:56.65 AS.reconfigure ASconfig
@02:47:56.70 AS.seek 0.47ml
@02:48:17.08 AFV.select :carrierSolution
@02:48:17.18 delay 2 seconds
@02:48:19.21 AS.seek 1ml
@02:48:49.99 delay 1 second
@02:48:51.01 AFV.select :holdingCoil
@02:48:51.11 delay 1 second
@02:48:52.14 AS.reconfigure ASfast
@02:48:52.18 AS.seek 0ml
@02:49:19.53 AFV.select :carrierSolution
@02:49:19.63 delay 2 seconds
@02:49:21.65 AS.reconfigure ASconfig
@02:49:21.70 AS.seek 1ml
@02:50:19.44 delay 1 second
@02:50:20.46 AFV.select :holdingCoil
@02:50:20.56 delay 1 second
@02:50:22.00 AS.reconfigure ASfast
@02:50:22.06 AS.seek 0ml
@02:50:49.38 AFV.select :carrierSolution
@02:50:49.48 delay 2 seconds
@02:50:51.51 AS.reconfigure ASconfig
@02:50:51.55 AS.seek 1ml
@02:51:49.27 delay 1 second
@02:51:50.29 AFV.select :holdingCoil
@02:51:50.39 delay 1 second
@02:51:51.43 AS.reconfigure ASfast
@02:51:51.47 AS.seek 0ml
@02:52:18.80 ARV.dial between carrier and air1
@02:52:19.31 ATV.dial :air3
@02:52:20.66 AS.reconfigure ASslow1
@02:52:20.70 AS.seek 0.01ml
@02:52:23.11 delay 2 seconds
@02:52:25.13 ATV.dial :bleach
@02:52:25.77 AS.seek 0.81ml
@02:55:06.89 delay 3 seconds
@02:55:09.91 ATV.dial :air3
@02:55:10.49 delay 3 seconds
@02:55:13.56 AS.seek 0.835ml
@02:55:18.84 delay 3 seconds
@02:55:21.86 ATV.dial :bleach
@02:55:22.50 AS.seek 0.82ml
@02:55:25.91 delay 3 seconds
@02:55:28.93 ATV.dial PCR
@02:55:29.33 AS.reconfigure ASconfig
@02:55:29.38 AS.seek 0ml
@02:56:16.80 PCR.program :bleachProfile
@02:56:16.86 PCR program should complete in < 2:45
@02:56:16.87 PCR.run 10:00
@02:59:18.71 PCR.off
@02:59:18.74 AFV.select :carrierSolution
@02:59:18.84 delay 2 seconds
@02:59:20.87 AS.seek 1ml
@03:00:18.61 delay 1 second
@03:00:19.63 AFV.select :holdingCoil
@03:00:19.73 delay 1 second
@03:00:20.76 AS.reconfigure ASfast
@03:00:20.80 AS.seek 0ml
@03:00:48.13 delay 3 seconds
@03:00:51.15 AFV.select :carrierSolution
@03:00:51.25 delay 2 seconds
@03:00:53.27 AS.reconfigure ASconfig
@03:00:53.33 AS.seek 1ml
@03:01:51.07 delay 1 second
@03:01:52.10 AFV.select :holdingCoil
@03:01:52.20 delay 1 second
@03:01:53.23 AS.reconfigure ASfast
@03:01:53.27 AS.seek 0ml
@03:02:20.60 delay 3 seconds
@03:02:23.62 AFV.select :carrierSolution
@03:02:23.72 delay 2 seconds
@03:02:25.75 AS.reconfigure ASconfig
@03:02:25.79 AS.seek 1ml
@03:03:23.53 delay 1 second
@03:03:24.55 AFV.select :holdingCoil
@03:03:24.65 delay 1 second
@03:03:25.68 AS.reconfigure ASfast
@03:03:26.12 AS.seek 0ml
@03:03:53.47 delay 3 seconds
@03:03:56.49 AFV.select :carrierSolution
@03:03:56.59 delay 2 seconds
@03:03:58.62 AS.reconfigure ASconfig
@03:03:58.67 AS.seek 1ml
@03:04:56.41 delay 1 second
@03:04:57.43 AFV.select :holdingCoil
@03:04:57.53 delay 1 second
@03:04:58.56 AS.reconfigure ASfast
@03:04:58.60 AS.seek 0ml
@03:05:25.94 delay 3 seconds
@03:05:28.96 AFV.select :carrierSolution
@03:05:29.06 delay 2 seconds
@03:05:31.09 AS.reconfigure ASconfig
@03:05:31.14 AS.seek 1ml
@03:06:28.88 delay 1 second
@03:06:29.90 AFV.select :holdingCoil
@03:06:30.00 delay 1 second
@03:06:31.03 AS.reconfigure ASnorm
@03:06:31.07 AS.seek 0ml
@03:07:21.80 AFV.select :carrierSolution
@03:07:21.90 delay 2 seconds
@03:07:23.92 AS.reconfigure ASconfig
@03:07:23.97 AS.seek 1ml
@03:08:21.72 delay 1 second
@03:08:22.74 AFV.select :holdingCoil
@03:08:22.84 delay 1 second
@03:08:23.86 ATV.dial :waste
@03:08:25.14 AS.reconfigure ASnorm
@03:08:25.19 AS.seek 0.5ml
@03:08:50.77 ATV.dial :waste2
@03:08:52.10 AS.seek 0ml
@03:09:17.69 <MAIN> Cleaning PCR
@03:09:17.70 AFV.select :carrierSolution
@03:09:17.80 delay 2 seconds
@03:09:19.83 AS.reconfigure ASconfig
@03:09:19.87 AS.seek 0.5ml
@03:09:48.91 delay 1 second
@03:09:49.93 AFV.select :holdingCoil
@03:09:50.03 ASV.dial :waste1
@03:09:50.04 ATV.dial :air3
@03:09:52.07 AS.reconfigure ASslow1
@03:09:52.12 AS.seek 0.51ml
@03:09:54.42 delay 2 seconds
@03:09:56.44 ATV.dial :bleach
@03:09:56.91 AS.seek 0.81ml
@03:10:57.63 delay 3 seconds
@03:11:00.65 ATV.dial :air3
@03:11:01.24 delay 3 seconds
@03:11:04.31 AS.seek 0.835ml
@03:11:09.60 delay 3 seconds
@03:11:12.62 ATV.dial :bleach
@03:11:13.25 AS.seek 0.82ml
@03:11:16.53 delay 3 seconds
@03:11:19.55 ARV.dial :waste
@03:11:20.06 delay 3 seconds
@03:11:23.08 ATV.dial :ARV
@03:11:23.99 AS.reconfigure ASconfig
@03:11:24.03 AS.seek 0.47ml
@03:11:44.42 AFV.select :carrierSolution
@03:11:44.52 delay 2 seconds
@03:11:46.55 AS.seek 1ml
@03:12:17.31 delay 1 second
@03:12:18.33 AFV.select :holdingCoil
@03:12:18.43 delay 1 second
@03:12:19.45 AS.reconfigure ASfast
@03:12:19.50 AS.seek 0ml
@03:12:46.83 AFV.select :carrierSolution
@03:12:46.93 delay 2 seconds
@03:12:48.95 AS.reconfigure ASconfig
@03:12:49.00 AS.seek 1ml
@03:13:46.74 delay 1 second
@03:13:47.76 AFV.select :holdingCoil
@03:13:47.86 delay 1 second
@03:13:48.89 AS.reconfigure ASfast
@03:13:48.93 AS.seek 0ml
@03:14:16.27 AFV.select :carrierSolution
@03:14:16.37 delay 2 seconds
@03:14:18.39 AS.reconfigure ASconfig
@03:14:18.44 AS.seek 1ml
@03:15:16.22 delay 1 second
@03:15:17.23 AFV.select :holdingCoil
@03:15:17.33 delay 1 second
@03:15:18.35 AS.reconfigure ASfast
@03:15:18.40 AS.seek 0ml
@03:15:45.72 ARV.dial between carrier and air1
@03:15:46.24 ATV.dial :air3
@03:15:47.57 AS.reconfigure ASslow1
@03:15:47.62 AS.seek 0.01ml
@03:15:50.02 delay 2 seconds
@03:15:52.04 ATV.dial :bleach
@03:15:52.67 AS.seek 0.81ml
@03:18:33.80 delay 3 seconds
@03:18:36.82 ATV.dial :air3
@03:18:37.39 delay 3 seconds
@03:18:40.46 AS.seek 0.835ml
@03:18:45.77 delay 3 seconds
@03:18:48.79 ATV.dial :bleach
@03:18:49.42 AS.seek 0.82ml
@03:18:52.83 delay 3 seconds
@03:18:55.85 ATV.dial PCR
@03:18:56.25 AS.reconfigure ASconfig
@03:18:56.29 AS.seek 0ml
@03:19:43.70 PCR.program :bleachProfile
@03:19:43.75 PCR program should complete in < 2:45
@03:19:43.76 PCR.run 10:00
@03:22:42.93 PCR.off
@03:22:42.96 AFV.select :carrierSolution
@03:22:43.06 delay 2 seconds
@03:22:45.09 AS.seek 1ml
@03:23:42.85 delay 1 second
@03:23:43.87 AFV.select :holdingCoil
@03:23:43.97 delay 1 second
@03:23:45.00 AS.reconfigure ASfast
@03:23:45.04 AS.seek 0ml
@03:24:12.38 delay 3 seconds
@03:24:15.40 AFV.select :carrierSolution
@03:24:15.50 delay 2 seconds
@03:24:17.52 AS.reconfigure ASconfig
@03:24:17.57 AS.seek 1ml
@03:25:15.35 delay 1 second
@03:25:16.37 AFV.select :holdingCoil
@03:25:16.47 delay 1 second
@03:25:17.50 AS.reconfigure ASfast
@03:25:17.54 AS.seek 0ml
@03:25:44.88 delay 3 seconds
@03:25:47.90 AFV.select :carrierSolution
@03:25:48.00 delay 2 seconds
@03:25:50.02 AS.reconfigure ASconfig
@03:25:50.07 AS.seek 1ml
@03:26:47.89 delay 1 second
@03:26:48.91 AFV.select :holdingCoil
@03:26:49.01 delay 1 second
@03:26:50.03 AS.reconfigure ASfast
@03:26:50.08 AS.seek 0ml
@03:27:17.41 delay 3 seconds
@03:27:20.43 AFV.select :carrierSolution
@03:27:20.53 delay 2 seconds
@03:27:22.55 AS.reconfigure ASconfig
@03:27:22.60 AS.seek 1ml
@03:28:20.33 delay 1 second
@03:28:21.35 AFV.select :holdingCoil
@03:28:21.45 delay 1 second
@03:28:22.48 AS.reconfigure ASfast
@03:28:22.52 AS.seek 0ml
@03:28:49.85 delay 3 seconds
@03:28:52.87 AFV.select :carrierSolution
@03:28:52.97 delay 2 seconds
@03:28:54.99 AS.reconfigure ASconfig
@03:28:55.04 AS.seek 1ml
@03:29:52.77 delay 1 second
@03:29:53.79 AFV.select :holdingCoil
@03:29:53.89 delay 1 second
@03:29:54.92 AS.reconfigure ASnorm
@03:29:54.96 AS.seek 0ml
@03:30:45.65 delay 5 seconds
@03:30:50.72 Cleaning PCR
@03:30:50.73 AFV.select :carrierSolution
@03:30:50.83 delay 2 seconds
@03:30:52.85 AS.reconfigure ASconfig
@03:30:52.90 AS.seek 0.5ml
@03:31:21.97 delay 1 second
@03:31:22.99 AFV.select :holdingCoil
@03:31:23.09 ASV.dial :waste1
@03:31:23.10 ATV.dial :air3
@03:31:23.97 AS.reconfigure ASslow1
@03:31:24.01 AS.seek 0.51ml
@03:31:26.38 delay 2 seconds
@03:31:28.40 ATV.dial :bleach
@03:31:29.03 AS.seek 0.81ml
@03:32:29.64 delay 3 seconds
@03:32:32.66 ATV.dial :air3
@03:32:33.24 delay 3 seconds
@03:32:36.31 AS.seek 0.835ml
@03:32:41.66 delay 3 seconds
@03:32:44.68 ATV.dial :bleach
@03:32:45.30 AS.seek 0.82ml
@03:32:48.60 delay 3 seconds
@03:32:51.62 ARV.dial :waste
@03:32:52.13 delay 3 seconds
@03:32:55.15 ATV.dial :ARV
@03:32:56.05 AS.reconfigure ASconfig
@03:32:56.09 AS.seek 0.47ml
@03:33:16.49 AFV.select :carrierSolution
@03:33:16.59 delay 2 seconds
@03:33:18.62 AS.seek 1ml
@03:33:49.39 delay 1 second
@03:33:50.41 AFV.select :holdingCoil
@03:33:50.51 delay 1 second
@03:33:51.54 AS.reconfigure ASfast
@03:33:51.58 AS.seek 0ml
@03:34:18.91 AFV.select :carrierSolution
@03:34:19.01 delay 2 seconds
@03:34:21.03 AS.reconfigure ASconfig
@03:34:21.08 AS.seek 1ml
@03:35:18.81 delay 1 second
@03:35:19.83 AFV.select :holdingCoil
@03:35:19.93 delay 1 second
@03:35:20.96 AS.reconfigure ASfast
@03:35:21.00 AS.seek 0ml
@03:35:48.33 AFV.select :carrierSolution
@03:35:48.43 delay 2 seconds
@03:35:50.46 AS.reconfigure ASconfig
@03:35:50.50 AS.seek 1ml
@03:36:48.33 delay 1 second
@03:36:49.35 AFV.select :holdingCoil
@03:36:49.45 delay 1 second
@03:36:50.48 AS.reconfigure ASfast
@03:36:50.55 AS.seek 0ml
@03:37:17.88 ARV.dial between carrier and air1
@03:37:18.39 ATV.dial :air3
@03:37:19.73 AS.reconfigure ASslow1
@03:37:19.77 AS.seek 0.01ml
@03:37:22.19 delay 2 seconds
@03:37:24.21 ATV.dial :bleach
@03:37:24.84 AS.seek 0.81ml
@03:40:06.00 delay 3 seconds
@03:40:09.02 ATV.dial :air3
@03:40:09.60 delay 3 seconds
@03:40:12.67 AS.seek 0.835ml
@03:40:17.98 delay 3 seconds
@03:40:21.00 ATV.dial :bleach
@03:40:21.64 AS.seek 0.82ml
@03:40:24.92 delay 3 seconds
@03:40:27.94 ATV.dial PCR
@03:40:28.35 AS.reconfigure ASconfig
@03:40:28.39 AS.seek 0ml
@03:41:15.80 PCR.program :bleachProfile
@03:41:15.85 PCR program should complete in < 2:45
@03:41:15.86 PCR.run 10:00
@03:44:14.69 PCR.off
@03:44:14.72 AFV.select :carrierSolution
@03:44:14.82 delay 2 seconds
@03:44:16.85 AS.seek 1ml
@03:45:14.66 delay 1 second
@03:45:15.68 AFV.select :holdingCoil
@03:45:15.78 delay 1 second
@03:45:16.81 AS.reconfigure ASfast
@03:45:16.85 AS.seek 0ml
@03:45:44.19 delay 3 seconds
@03:45:47.21 AFV.select :carrierSolution
@03:45:47.31 delay 2 seconds
@03:45:49.33 AS.reconfigure ASconfig
@03:45:49.38 AS.seek 1ml
@03:46:47.13 delay 1 second
@03:46:48.15 AFV.select :holdingCoil
@03:46:48.25 delay 1 second
@03:46:49.28 AS.reconfigure ASfast
@03:46:49.32 AS.seek 0ml
@03:47:16.64 delay 3 seconds
@03:47:19.66 AFV.select :carrierSolution
@03:47:19.76 delay 2 seconds
@03:47:21.78 AS.reconfigure ASconfig
@03:47:21.84 AS.seek 1ml
@03:48:19.63 delay 1 second
@03:48:20.65 AFV.select :holdingCoil
@03:48:20.75 delay 1 second
@03:48:21.77 AS.reconfigure ASfast
@03:48:21.82 AS.seek 0ml
@03:48:49.14 delay 3 seconds
@03:48:52.16 AFV.select :carrierSolution
@03:48:52.26 delay 2 seconds
@03:48:54.28 AS.reconfigure ASconfig
@03:48:54.33 AS.seek 1ml
@03:49:52.11 delay 1 second
@03:49:53.12 AFV.select :holdingCoil
@03:49:53.22 delay 1 second
@03:49:54.25 AS.reconfigure ASfast
@03:49:54.29 AS.seek 0ml
@03:50:21.63 delay 3 seconds
@03:50:24.65 AFV.select :carrierSolution
@03:50:24.75 delay 2 seconds
@03:50:26.77 AS.reconfigure ASconfig
@03:50:26.83 AS.seek 1ml
@03:51:24.61 delay 1 second
@03:51:25.63 AFV.select :holdingCoil
@03:51:25.73 delay 1 second
@03:51:27.08 AS.reconfigure ASnorm
@03:51:27.14 AS.seek 0ml
@03:52:17.88 delay 5 seconds
@03:52:22.96 Cleaning PCR
AFV.select :carrierSolution
@03:52:23.06 delay 2 seconds
@03:52:25.09 AS.reconfigure ASconfig
@03:52:25.13 AS.seek 0.5ml
@03:52:54.16 delay 1 second
@03:52:55.18 AFV.select :holdingCoil
@03:52:55.28 ASV.dial :waste1
@03:52:55.29 ATV.dial :air3
@03:52:56.18 AS.reconfigure ASslow1
@03:52:56.23 AS.seek 0.51ml
@03:52:58.58 delay 2 seconds
@03:53:00.60 ATV.dial :bleach
@03:53:01.23 AS.seek 0.81ml
@03:54:01.83 delay 3 seconds
@03:54:04.85 ATV.dial :air3
@03:54:05.42 delay 3 seconds
@03:54:08.49 AS.seek 0.835ml
@03:54:13.87 delay 3 seconds
@03:54:16.89 ATV.dial :bleach
@03:54:17.53 AS.seek 0.82ml
@03:54:20.92 delay 3 seconds
@03:54:23.94 ARV.dial :waste
@03:54:24.45 delay 3 seconds
@03:54:27.47 ATV.dial :ARV
@03:54:28.38 AS.reconfigure ASconfig
@03:54:28.43 AS.seek 0.47ml
@03:54:48.83 AFV.select :carrierSolution
@03:54:48.93 delay 2 seconds
@03:54:50.96 AS.seek 1ml
@03:55:21.74 delay 1 second
@03:55:22.76 AFV.select :holdingCoil
@03:55:22.86 delay 1 second
@03:55:23.89 AS.reconfigure ASfast
@03:55:23.93 AS.seek 0ml
@03:55:51.27 AFV.select :carrierSolution
@03:55:51.37 delay 2 seconds
@03:55:53.39 AS.reconfigure ASconfig
@03:55:53.44 AS.seek 1ml
@03:56:51.20 delay 1 second
@03:56:52.22 AFV.select :holdingCoil
@03:56:52.32 delay 1 second
@03:56:53.34 AS.reconfigure ASfast
@03:56:53.39 AS.seek 0ml
@03:57:20.72 AFV.select :carrierSolution
@03:57:20.82 delay 2 seconds
@03:57:22.84 AS.reconfigure ASconfig
@03:57:22.89 AS.seek 1ml
@03:58:20.66 delay 1 second
@03:58:21.68 AFV.select :holdingCoil
@03:58:21.78 delay 1 second
@03:58:22.81 AS.reconfigure ASfast
@03:58:22.85 AS.seek 0ml
@03:58:50.20 ARV.dial between carrier and air1
@03:58:50.72 ATV.dial :air3
@03:58:52.07 AS.reconfigure ASslow1
@03:58:52.12 AS.seek 0.01ml
@03:58:54.52 delay 2 seconds
@03:58:56.54 ATV.dial :bleach
@03:58:57.17 AS.seek 0.81ml
@04:01:38.42 delay 3 seconds
@04:01:41.44 ATV.dial :air3
@04:01:42.02 delay 3 seconds
@04:01:45.09 AS.seek 0.835ml
@04:01:50.37 delay 3 seconds
@04:01:53.39 ATV.dial :bleach
@04:01:54.03 AS.seek 0.82ml
@04:01:57.33 delay 3 seconds
@04:02:00.35 ATV.dial PCR
@04:02:00.75 AS.reconfigure ASconfig
@04:02:00.79 AS.seek 0ml
@04:02:48.17 PCR.program :bleachProfile
@04:02:48.22 PCR program should complete in < 2:45
@04:02:48.23 PCR.run 10:00
@04:05:47.39 PCR.off
@04:05:47.42 AFV.select :carrierSolution
@04:05:47.52 delay 2 seconds
@04:05:49.55 AS.seek 1ml
@04:06:47.28 delay 1 second
@04:06:48.30 AFV.select :holdingCoil
@04:06:48.40 delay 1 second
@04:06:49.43 AS.reconfigure ASfast
@04:06:49.47 AS.seek 0ml
@04:07:16.81 delay 3 seconds
@04:07:19.83 AFV.select :carrierSolution
@04:07:19.93 delay 2 seconds
@04:07:21.95 AS.reconfigure ASconfig
@04:07:22.01 AS.seek 1ml
@04:08:19.75 delay 1 second
@04:08:20.77 AFV.select :holdingCoil
@04:08:20.87 delay 1 second
@04:08:21.90 AS.reconfigure ASfast
@04:08:21.94 AS.seek 0ml
@04:08:49.27 delay 3 seconds
@04:08:52.29 AFV.select :carrierSolution
@04:08:52.39 delay 2 seconds
@04:08:54.41 AS.reconfigure ASconfig
@04:08:54.46 AS.seek 1ml
@04:09:52.19 delay 1 second
@04:09:53.21 AFV.select :holdingCoil
@04:09:53.31 delay 1 second
@04:09:54.34 AS.reconfigure ASfast
@04:09:54.38 AS.seek 0ml
@04:10:21.72 delay 3 seconds
@04:10:24.74 AFV.select :carrierSolution
@04:10:24.84 delay 2 seconds
@04:10:26.86 AS.reconfigure ASconfig
@04:10:26.91 AS.seek 1ml
@04:11:24.64 delay 1 second
@04:11:25.66 AFV.select :holdingCoil
@04:11:25.76 delay 1 second
@04:11:26.79 AS.reconfigure ASfast
@04:11:26.83 AS.seek 0ml
@04:11:54.16 delay 3 seconds
@04:11:57.18 AFV.select :carrierSolution
@04:11:57.28 delay 2 seconds
@04:11:59.30 AS.reconfigure ASconfig
@04:11:59.35 AS.seek 1ml
@04:12:57.09 delay 1 second
@04:12:58.11 AFV.select :holdingCoil
@04:12:58.21 delay 1 second
@04:12:59.24 AS.reconfigure ASnorm
@04:12:59.28 AS.seek 0ml
@04:13:50.02 delay 5 seconds
nohup: ESP: No such file or directory
@14:37:13.93CEST21-Apr-21 <MAIN> ESP2 v3.30 I2C Gateway v3.06 for core bus
@14:37:16.84 
Configuration for mer 7/20/20 brent@mbari.org
/home/esp/esp2/type/shallow/mer/configure.rb
@14:37:16.93 
Configuration for MFB3 by preston@mbari.org
/home/esp/esp2/type/mfb/MFB3/configure.rb
@14:37:22.88 Polling Can every 10:00
@14:37:24.66 CS.configure :default
@14:37:24.72 CS.hold
@14:37:24.82 CS.configure :default
@14:37:25.00 PS.configure :default
@14:37:25.16 SS.configure :default
@14:37:25.22 SS.hold
@14:37:25.32 SS.configure :default
@14:37:25.44 CC.configure :default
@14:37:25.48 CC.hold
@14:37:25.55 CC.configure :default
@14:37:25.63 PC.configure :default
@14:37:25.66 PC.hold
@14:37:25.73 PC.configure :default
@14:37:25.82 Forearm.configure :loose
@14:37:25.86 Forearm.hold
@14:37:25.92 Forearm.configure :loose
@14:37:26.04 Elbow.configure :loose
@14:37:26.10 Elbow.hold
@14:37:26.20 Elbow.configure :loose
@14:37:26.40 SC.configure :loose
@14:37:26.48 SC.hold
@14:37:26.58 SC.configure :loose
@14:37:26.74 SE.configure :default
@14:37:26.80 SE.hold
@14:37:26.86 SE.configure :default
@14:37:26.89 CH.coast
@14:37:26.94 CH.configure :default
@14:37:26.97 PH.coast
@14:37:27.01 PH.configure :default
@14:37:27.11 SPE.coast
@14:37:27.16 SPE.configure SPEconfig
@14:37:27.23 AS.configure ASconfig
@14:37:27.27 AS.hold
@14:37:27.33 AS.configure ASconfig
@14:37:27.37 PCR.off
@14:37:27.43 PCR.pidtemp= 50,0.1,0,300,25,25
@14:37:27.50 PCR.caltemp= 2.5051,0.0372,0
@14:37:27.56 All dwarf controllers running firmware version 3.75
@14:37:27.95 Cleanly exited at 14:35:00CEST21-Apr-21
@14:37:28.03 Loading /home/esp/esp2/mission/GENOMAP_v3.rb mission
@14:37:28.31 <MFBPCR> Extracting Nucleic Acid from extSrc
@14:37:28.37 SPE.hold 2:46:40,55C
@14:37:28.43 ASV.dial :SPEdelivery
@14:37:28.57 <bkha> Session Begun
@14:37:28.92 <MFBPCR> delay 20 seconds
@14:37:48.95 AFV.select :holdingCoil
@14:37:49.05 ATV.dial :air2
@14:37:50.53 AS.reconfigure ASslow1
@14:37:50.59 AS.seek 0.07ml
@14:38:04.99 delay 3 seconds
@14:38:08.01 ATV.dial :extSrc
@14:38:09.52 AS.seek 0.47ml
@14:39:30.27 delay 5 seconds
@14:39:35.34 Solid Phase Extraction Heater at 40.5C
@14:39:35.35 ATV.dial ASV
@14:39:36.24 ASV.dial :SPEdelivery
@14:39:36.32 AS.seek 0.042ml
@14:41:02.74 delay 10 seconds
@14:41:12.77 Rinsing external lysate line extSrc
@14:41:12.78 AFV.select :carrierSolution
@14:41:12.92 delay 2 seconds
@14:41:14.95 AS.reconfigure ASconfig
@14:41:15.00 AS.seek 1ml
@14:42:10.33 delay 1 second
@14:42:11.35 AFV.select :holdingCoil
@14:42:11.45 delay 1 second
@14:42:12.47 ATV.dial :extSrc
@14:42:13.28 AS.reconfigure ASnorm
@14:42:13.34 AS.seek 0ml
@14:43:04.03 ATV.dial :air2
@14:43:05.61 AS.reconfigure ASslow0
@14:43:05.67 AS.seek 0.05ml
@14:43:11.06 ATV.dial :extSrc
@14:43:12.64 AS.seek 0.01ml
@14:43:17.03 ATV.dial :waste
@14:43:18.79 AS.reconfigure ASconfig
@14:43:18.90 AS.seek 0ml
@14:43:19.75 ethanol wash
@14:43:19.76 ATV.dial :air2
@14:43:20.98 AS.reconfigure ASslow2
@14:43:21.04 AS.seek 0.01ml
@14:43:31.56 delay 3 seconds
@14:43:34.58 Pulling 0.4ml of Ethanol Wash
@14:43:34.59 ATV.dial :ethanol
@14:43:35.50 AS.reconfigure ASslow1
@14:43:35.55 AS.seek 0.41ml
@14:44:56.39 delay 3 seconds
@14:44:59.41 ATV.dial :air1
@14:44:59.83 delay 3 seconds
@14:45:02.90 AS.seek 0.435ml
@14:45:08.15 delay 3 seconds
@14:45:11.17 ATV.dial :ethanol
@14:45:11.81 AS.seek 0.42ml
@14:45:15.14 delay 3 seconds
@14:45:18.16 ATV.dial ASV
@14:45:19.59 AS.seek 0.093ml
@14:46:25.63 delay 3 seconds
@14:46:28.65 ATV.dial :waste2
@14:46:29.47 AS.reconfigure ASconfig
@14:46:29.53 AS.seek 0ml
@14:46:35.16 ATV.dial :air2
@14:46:35.58 AS.reconfigure ASslow1
@14:46:35.64 AS.seek 1ml
@14:49:57.06 delay 5 seconds
@14:50:02.08 ATV.dial ASV
@14:50:03.13 ASV.dial :SPEdelivery
@14:50:03.15 AS.seek 0.02ml
@14:53:20.52 delay 5 seconds
@14:53:25.54 ATV.dial :air2
@14:53:26.64 AS.seek 0.52ml
@14:55:07.55 delay 5 seconds
@14:55:12.57 ATV.dial ASV
@14:55:13.61 ASV.dial :wasteR
@14:55:14.00 AS.seek 0.3ml
@14:55:58.56 delay 20 seconds
@14:56:18.60 AS.seek 0.07ml
@14:57:05.11 delay 20 seconds
@14:57:25.13 ATV.dial :waste2
@14:57:25.94 AS.reconfigure ASconfig
@14:57:26.00 AS.seek 0ml
@14:57:30.33 Elution
@14:57:30.34 ATV.dial :air1
@14:57:31.54 AS.reconfigure ASslow1
@14:57:31.60 AS.seek 0.05ml
@14:57:41.94 delay 3 seconds
@14:57:44.96 ATV.dial :ElutionWater
@14:57:45.69 AS.seek 0.11ml
@14:57:58.06 delay 3 seconds
@14:58:01.08 ATV.dial ASV,via: :air2
@14:58:03.32 ASV.dial :SPEelution
@14:58:03.94 AFV.select :carrierSolution
@14:58:04.10 AS.reconfigure ASnorm
@14:58:04.15 AS.seek 0.76ml
@14:58:37.25 delay 3 seconds
@14:58:40.27 AFV.select :holdingCoil
@14:58:40.42 AS.reconfigure ASslow1
@14:58:40.47 AS.seek 0.475ml
@14:59:38.08 delay 5 seconds
@14:59:43.16 AS.reconfigure ASslow2
@14:59:43.22 AS.seek 0.44ml
@15:00:18.80 delay 1:00
@15:01:18.83 pushing eluate to holding coil
@15:01:18.90 AS.seek 0.28ml
@15:04:00.16 delay 30 seconds
@15:04:30.19 ATV.dial :waste,via: :air3
@15:04:32.05 AS.reconfigure ASconfig
@15:04:32.11 AS.seek 0ml
@15:04:48.53 Parking Bleach in LysateSource line and SPEcolumn
@15:04:48.54 ATV.dial :air3
@15:04:49.44 AS.reconfigure ASslow1
@15:04:49.50 AS.seek 0.03ml
@15:04:55.85 ATV.dial :ElutionWater
@15:04:56.50 AS.seek 0.06ml
@15:05:02.78 ATV.dial :air3
@15:05:03.42 AS.seek 0.07ml
@15:05:05.75 delay 2 seconds
@15:05:07.77 ATV.dial :bleach
@15:05:08.26 AS.seek 0.15ml
@15:05:24.64 delay 3 seconds
@15:05:27.66 ATV.dial :air3
@15:05:28.24 delay 3 seconds
@15:05:31.31 AS.seek 0.175ml
@15:05:36.70 delay 3 seconds
@15:05:39.72 ATV.dial :bleach
@15:05:40.36 AS.seek 0.16ml
@15:05:43.66 delay 3 seconds
@15:05:46.68 ATV.dial :extSrc
@15:05:47.33 AS.reconfigure ASconfig
@15:05:47.39 AS.seek 0.015ml
@15:05:56.03 ATV.dial :waste
@15:05:57.56 AS.seek 0ml
@15:05:58.74 ATV.dial :air3
@15:05:59.63 AS.reconfigure ASslow1
@15:05:59.69 AS.seek 0.01ml
@15:06:02.19 delay 2 seconds
@15:06:04.21 ATV.dial :bleach
@15:06:04.68 AS.seek 0.58ml
@15:07:59.60 delay 3 seconds
@15:08:02.62 ATV.dial :air3
@15:08:03.20 delay 3 seconds
@15:08:06.27 AS.seek 0.605ml
@15:08:11.72 delay 3 seconds
@15:08:14.74 ATV.dial :bleach
@15:08:15.39 AS.seek 0.59ml
@15:08:18.78 delay 3 seconds
@15:08:21.80 ATV.dial :ASV
@15:08:22.89 ASV.dial :wasteR
@15:08:23.50 AS.seek 0ml
@15:10:22.45 delay 1:00
@15:11:22.48 AFV.select :carrierSolution
@15:11:22.58 delay 2 seconds
@15:11:24.60 AS.reconfigure ASconfig
@15:11:24.66 AS.seek 1ml
@15:12:22.42 delay 1 second
@15:12:23.44 AFV.select :holdingCoil
@15:12:23.54 delay 1 second
@15:12:24.56 ATV.dial ASV
@15:12:24.57 ASV.dial :wasteR
@15:12:24.60 AS.reconfigure ASslow1
@15:12:24.66 AS.seek 0ml
@15:15:46.06 delay 20 seconds
@15:16:06.09 ATV.dial :air3
@15:16:07.70 AS.seek 0.59ml
@15:18:06.69 delay 3 seconds
@15:18:09.71 ATV.dial :ASV
@15:18:11.24 ASV.dial :wasteR
@15:18:11.32 AS.seek 0.09ml
@15:19:52.16 delay 30 seconds
@15:20:22.19 ATV.dial :extSrc
@15:20:23.00 delay 3 seconds
@15:20:26.02 ATV.dial :ElutionWater
@15:20:27.22 AS.seek 0.12ml
@15:20:33.58 ATV.dial :air3
@15:20:34.23 AS.seek 0.13ml
@15:20:36.55 delay 2 seconds
@15:20:38.57 ATV.dial :bleach
@15:20:39.06 AS.seek 0.23ml
@15:20:59.42 delay 3 seconds
@15:21:02.44 ATV.dial :air3
@15:21:03.05 delay 3 seconds
@15:21:06.12 AS.seek 0.255ml
@15:21:11.48 delay 3 seconds
@15:21:14.50 ATV.dial :bleach
@15:21:15.57 AS.seek 0.24ml
@15:21:19.03 delay 3 seconds
@15:21:22.05 ATV.dial :ASV
@15:21:23.16 ASV.dial :SPEdelivery
@15:21:23.56 AFV.select :carrierSolution
@15:21:23.67 delay 2 seconds
@15:21:25.70 AS.reconfigure ASconfig
@15:21:25.75 AS.seek 0.5ml
@15:21:40.97 delay 1 second
@15:21:41.99 AFV.select :holdingCoil
@15:21:42.14 AS.reconfigure ASslow1
@15:21:42.19 AS.seek 0.17ml
@15:22:49.00 SPE.hold 2:46:40,95C
@15:22:49.05 delay 3:00
@15:25:49.10 AFV.select :carrierSolution
@15:25:49.21 delay 2 seconds
@15:25:51.23 AS.reconfigure ASconfig
@15:25:51.29 AS.seek 1ml
@15:26:39.33 delay 1 second
@15:26:40.35 AFV.select :holdingCoil
@15:26:40.45 delay 1 second
@15:26:41.47 ASV.dial :wasteD
@15:26:42.58 AS.seek 0ml
@15:27:40.36 Priming Eluate
@15:27:40.37 AFV.select :carrierSolution
@15:27:40.49 delay 2 seconds
@15:27:42.52 AS.seek 0.5ml
@15:28:11.53 delay 1 second
@15:28:12.55 AFV.select :holdingCoil
@15:28:12.65 ATV.dial ASV
@15:28:12.67 ASV.dial :SPErecovery
@15:28:13.31 Swish #1 of 2
@15:28:13.39 AS.reconfigure ASslow0
@15:28:13.45 AS.seek 0.6ml
@15:28:23.90 AS.seek 0.5ml
@15:28:34.31 Swish #2 of 2
@15:28:34.39 AS.seek 0.6ml
@15:28:44.86 AS.seek 0.5ml
@15:28:55.30 delay 3 seconds
@15:28:58.37 AS.reconfigure ASslow1
@15:28:58.43 AS.seek 0.684ml
@15:29:35.74 delay 3 seconds
@15:29:38.76 Eluate primed
@15:29:38.77 ATV.dial :waste2
@15:29:39.45 AS.reconfigure ASnorm
@15:29:39.51 AS.seek 0ml
@15:30:14.31 SPE.coast
@15:30:14.39 ASV.dial :SPErecovery
@15:30:14.41 Setting up PCR for SYBRIPc -- writing /var/log/esp/SYBRIPc21apr2115h.pcr
@15:30:14.43 Building PCR Slug from ASV with MM1 mix, PP6 primer
@15:30:14.45 Priming with carrier
@15:30:14.47 ATV.dial ARV
@15:30:15.52 ARV.dial :carrier
@15:30:15.87 AS.seek 0.5ml
@15:30:41.47 AFV.select :carrierSolution
@15:30:41.59 delay 2 seconds
@15:30:43.62 AS.reconfigure ASconfig
@15:30:43.68 AS.seek 1ml
@15:31:12.70 delay 1 second
@15:31:13.72 AFV.select :holdingCoil
@15:31:13.82 delay 1 second
@15:31:14.84 ATV.dial :waste
@15:31:16.45 AS.reconfigure ASnorm
@15:31:16.51 AS.seek 0ml
@15:32:07.23 AFV.select :carrierSolution
@15:32:07.35 delay 2 seconds
@15:32:09.38 AS.reconfigure ASconfig
@15:32:09.43 AS.seek 0.5ml
@15:32:38.45 delay 1 second
@15:32:39.47 AFV.select :holdingCoil
@15:32:39.57 Building Slug
@15:32:39.59 ATV.dial :ARV
@15:32:41.36 delay 2 seconds
@15:32:43.38 ARV.dial :air1
@15:32:43.78 delay 2 seconds
@15:32:45.85 AS.reconfigure ASslow2
@15:32:45.91 AS.seek 0.506ml
@15:32:52.19 delay 2 seconds
@15:32:54.21 ARV.dial :MM1
@15:32:54.86 delay 2 seconds
@15:32:56.88 Adding MM1
@15:32:56.96 AS.seek 0.524ml
@15:33:15.42 delay 25 seconds
@15:33:40.44 ARV.dial :PP6
@15:33:41.80 Adding PP6
@15:33:41.81 delay 2 seconds
@15:33:43.89 AS.seek 0.53ml
@15:33:50.31 delay 10 seconds
@15:34:00.34 ARV.dial :air1
@15:34:01.22 adding ARV air
@15:34:01.23 delay 2 seconds
@15:34:03.31 AS.seek 0.536ml
@15:34:09.94 delay 5 seconds
@15:34:14.96 ARV.dial :carrier
@15:34:15.39 delay 7 seconds
@15:34:22.47 AS.seek 0.594ml
@15:35:21.42 delay 5 seconds
@15:35:26.44 ATV.dial :ASV
@15:35:26.89 delay 2 seconds
@15:35:28.96 AS.seek 0.6ml
@15:35:35.25 delay 4 seconds
@15:35:39.27 ATV.dial ARV
@15:35:39.86 delay 2 seconds
@15:35:41.88 ARV.dial :carrier
@15:35:41.89 delay 2 seconds
@15:35:43.97 AS.seek 0.65ml
@15:36:34.61 delay 4 seconds
@15:36:38.63 ATV.dial PCR
@15:36:39.27 ASV.dial :waste1
@15:36:40.09 ARV.dial :air1
@15:36:40.48 Positioning Slug by 0.324ml
@15:36:40.57 AS.seek 0.326ml
@15:42:06.66 delay 5 seconds
@15:42:11.68 ATV.dial ARV
@15:42:12.48 ASV.dial :PCRlock
@15:42:12.88 delay 5 seconds
@15:42:17.90 Running SYBRIPc Profile60CIQ1 thermocycling
@15:42:17.92 <PCR> PCR.program Profile60CIQ1
@15:42:18.03 Profile60CIQ1 should complete in < 1:56:15
@15:42:18.04 PCR.run 2:30:18.75
@16:02:52.55 <bkha#2> Session Begun
@16:02:56.43 -> showlog 100
@16:04:14.30 -> exit
@16:04:14.31 Session Ended
@17:22:15.18 <PCR> PCR.off
@17:22:15.73 <MFBPCR> PCR for SYBRIPc completed -- closed /var/log/esp/SYBRIPc21apr2115h.pcr
@17:22:15.81 Cleaning PCR
@17:22:15.82 AFV.select :carrierSolution
@17:22:15.94 delay 2 seconds
@17:22:17.97 AS.reconfigure ASconfig
@17:22:18.02 AS.seek 0.5ml
@17:22:28.31 delay 1 second
@17:22:29.33 AFV.select :holdingCoil
@17:22:29.43 ASV.dial :waste1
@17:22:29.84 ATV.dial :air3
@17:22:31.19 AS.reconfigure ASslow1
@17:22:31.26 AS.seek 0.51ml
@17:22:33.53 delay 2 seconds
@17:22:35.55 ATV.dial :bleach
@17:22:36.20 AS.seek 0.81ml
@17:23:36.81 delay 3 seconds
@17:23:39.83 ATV.dial :air3
@17:23:40.42 delay 3 seconds
@17:23:43.49 AS.seek 0.835ml
@17:23:48.79 delay 3 seconds
@17:23:51.81 ATV.dial :bleach
@17:23:52.84 AS.seek 0.82ml
@17:23:56.15 delay 3 seconds
@17:23:59.17 ARV.dial :waste
@17:23:59.81 delay 3 seconds
@17:24:02.83 ATV.dial :ARV
@17:24:03.78 AS.reconfigure ASconfig
@17:24:03.84 AS.seek 0.47ml
@17:24:24.23 AFV.select :carrierSolution
@17:24:24.34 delay 2 seconds
@17:24:26.37 AS.seek 1ml
@17:24:57.11 delay 1 second
@17:24:58.13 AFV.select :holdingCoil
@17:24:58.23 delay 1 second
@17:24:59.26 AS.reconfigure ASfast
@17:24:59.31 AS.seek 0ml
@17:25:26.65 AFV.select :carrierSolution
@17:25:26.76 delay 2 seconds
@17:25:28.79 AS.reconfigure ASconfig
@17:25:28.84 AS.seek 1ml
@17:26:26.62 delay 1 second
@17:26:27.64 AFV.select :holdingCoil
@17:26:27.74 delay 1 second
@17:26:28.77 AS.reconfigure ASfast
@17:26:28.82 AS.seek 0ml
@17:26:56.17 AFV.select :carrierSolution
@17:26:56.28 delay 2 seconds
@17:26:58.31 AS.reconfigure ASconfig
@17:26:58.36 AS.seek 1ml
@17:27:56.12 delay 1 second
@17:27:57.14 AFV.select :holdingCoil
@17:27:57.24 delay 1 second
@17:27:58.27 AS.reconfigure ASfast
@17:27:58.32 AS.seek 0ml
@17:28:25.67 ARV.dial between carrier and air1
@17:28:26.20 ATV.dial :air3
@17:28:27.57 AS.reconfigure ASslow1
@17:28:27.62 AS.seek 0.01ml
@17:28:30.03 delay 2 seconds
@17:28:32.05 ATV.dial :bleach
@17:28:32.70 AS.seek 0.81ml
@17:31:13.98 delay 3 seconds
@17:31:17.00 ATV.dial :air3
@17:31:17.59 delay 3 seconds
@17:31:20.66 AS.seek 0.835ml
@17:31:26.10 delay 3 seconds
@17:31:29.12 ATV.dial :bleach
@17:31:29.78 AS.seek 0.82ml
@17:31:33.07 delay 3 seconds
@17:31:36.09 ATV.dial PCR
@17:31:36.51 AS.reconfigure ASconfig
@17:31:36.57 AS.seek 0ml
@17:32:23.98 PCR.program :bleachProfile
@17:32:24.05 PCR program should complete in < 2:45
@17:32:24.06 PCR.run 10:00
@17:35:19.84 PCR.off
@17:35:19.88 AFV.select :carrierSolution
@17:35:19.99 delay 2 seconds
@17:35:22.02 AS.seek 1ml
@17:36:19.78 delay 1 second
@17:36:20.80 AFV.select :holdingCoil
@17:36:20.90 delay 1 second
@17:36:21.93 AS.reconfigure ASfast
@17:36:21.98 AS.seek 0ml
@17:36:49.32 delay 3 seconds
@17:36:52.34 AFV.select :carrierSolution
@17:36:52.44 delay 2 seconds
@17:36:54.46 AS.reconfigure ASconfig
@17:36:54.52 AS.seek 1ml
@17:37:52.31 delay 1 second
@17:37:53.33 AFV.select :holdingCoil
@17:37:53.43 delay 1 second
@17:37:54.46 AS.reconfigure ASfast
@17:37:54.51 AS.seek 0ml
@17:38:21.86 delay 3 seconds
@17:38:24.88 AFV.select :carrierSolution
@17:38:24.98 delay 2 seconds
@17:38:27.00 AS.reconfigure ASconfig
@17:38:27.07 AS.seek 1ml
@17:39:24.81 delay 1 second
@17:39:25.83 AFV.select :holdingCoil
@17:39:25.93 delay 1 second
@17:39:26.96 AS.reconfigure ASfast
@17:39:27.01 AS.seek 0ml
@17:39:54.34 delay 3 seconds
@17:39:57.36 AFV.select :carrierSolution
@17:39:57.46 delay 2 seconds
@17:39:59.49 AS.reconfigure ASconfig
@17:39:59.54 AS.seek 1ml
@17:40:57.28 delay 1 second
@17:40:58.30 AFV.select :holdingCoil
@17:40:58.40 delay 1 second
@17:40:59.43 AS.reconfigure ASfast
@17:40:59.48 AS.seek 0ml
@17:41:26.82 delay 3 seconds
@17:41:29.84 AFV.select :carrierSolution
@17:41:29.94 delay 2 seconds
@17:41:31.96 AS.reconfigure ASconfig
@17:41:32.44 AS.seek 1ml
@17:42:30.23 delay 1 second
@17:42:31.25 AFV.select :holdingCoil
@17:42:31.35 delay 1 second
@17:42:32.38 AS.reconfigure ASnorm
@17:42:32.43 AS.seek 0ml
@17:43:23.13 Setting up PCR for ACYPP2 -- writing /var/log/esp/ACYPP221apr2117h.pcr
@17:43:23.15 Building PCR Slug from ASV with MM1 mix, PP2 primer
@17:43:23.18 Priming with carrier
@17:43:23.19 ATV.dial ARV
@17:43:23.86 ARV.dial :carrier
@17:43:24.21 AS.seek 0.5ml
@17:43:49.86 AFV.select :carrierSolution
@17:43:49.98 delay 2 seconds
@17:43:52.01 AS.reconfigure ASconfig
@17:43:52.07 AS.seek 1ml
@17:44:21.09 delay 1 second
@17:44:22.11 AFV.select :holdingCoil
@17:44:22.21 delay 1 second
@17:44:23.23 ATV.dial :waste
@17:44:25.01 AS.reconfigure ASnorm
@17:44:25.07 AS.seek 0ml
@17:45:15.78 AFV.select :carrierSolution
@17:45:15.90 delay 2 seconds
@17:45:17.93 AS.reconfigure ASconfig
@17:45:17.98 AS.seek 0.5ml
@17:45:47.01 delay 1 second
@17:45:48.03 AFV.select :holdingCoil
@17:45:48.13 Building Slug
@17:45:48.15 ATV.dial :ARV
@17:45:49.90 delay 2 seconds
@17:45:51.92 ARV.dial :air1
@17:45:52.33 delay 2 seconds
@17:45:54.40 AS.reconfigure ASslow2
@17:45:54.46 AS.seek 0.506ml
@17:46:00.87 delay 2 seconds
@17:46:02.89 ARV.dial :MM1
@17:46:03.53 delay 2 seconds
@17:46:05.55 Adding MM1
@17:46:05.63 AS.seek 0.524ml
@17:46:23.98 delay 25 seconds
@17:46:49.00 ARV.dial :PP2
@17:46:49.43 Adding PP2
@17:46:49.44 delay 2 seconds
@17:46:51.52 AS.seek 0.53ml
@17:46:57.75 delay 10 seconds
@17:47:07.78 ARV.dial :air2
@17:47:08.20 adding ARV air
@17:47:08.21 delay 2 seconds
@17:47:10.29 AS.seek 0.536ml
@17:47:16.46 delay 5 seconds
@17:47:21.48 ARV.dial :carrier
@17:47:22.84 delay 7 seconds
@17:47:29.92 AS.seek 0.594ml
@17:48:28.53 delay 5 seconds
@17:48:33.55 ATV.dial :ASV
@17:48:33.96 delay 2 seconds
@17:48:36.03 AS.seek 0.6ml
@17:48:42.70 delay 4 seconds
@17:48:46.72 ATV.dial ARV
@17:48:47.40 delay 2 seconds
@17:48:49.42 ARV.dial :carrier
@17:48:49.43 delay 2 seconds
@17:48:51.51 AS.seek 0.65ml
@17:49:42.54 delay 4 seconds
@17:49:46.56 ATV.dial PCR
@17:49:47.21 ASV.dial :waste1
@17:49:47.22 ARV.dial :air1
@17:49:47.66 Positioning Slug by 0.324ml
@17:49:47.75 AS.seek 0.326ml
@17:55:14.28 delay 5 seconds
@17:55:19.30 ATV.dial ARV
@17:55:20.12 ASV.dial :PCRlock
@17:55:20.53 delay 5 seconds
@17:55:25.55 Running ACYPP2 Profile60CIQ2 thermocycling
@17:55:25.57 <PCR> PCR.program Profile60CIQ2
@17:55:25.68 Profile60CIQ2 should complete in < 1:47:50
@17:55:25.69 PCR.run 2:19:47.5
@19:24:21.73 PCR.off
@19:24:22.34 <MFBPCR> Running ACYPP2 MeltProfile60 thermocycling
@19:24:22.36 <PCR> PCR.program MeltProfile60
@19:24:22.46 MeltProfile60 should complete in < 11:55
@19:24:22.47 PCR.run 19:53.75
@19:32:15.33 PCR.off
@19:32:16.25 <MFBPCR> PCR for ACYPP2 completed -- closed /var/log/esp/ACYPP221apr2117h.pcr
@19:32:16.33 Cleaning PCR
@19:32:16.34 AFV.select :carrierSolution
@19:32:16.46 delay 2 seconds
@19:32:18.49 AS.reconfigure ASconfig
@19:32:18.54 AS.seek 0.5ml
@19:32:28.84 delay 1 second
@19:32:29.86 AFV.select :holdingCoil
@19:32:29.96 ASV.dial :waste1
@19:32:30.35 ATV.dial :air3
@19:32:31.71 AS.reconfigure ASslow1
@19:32:31.78 AS.seek 0.51ml
@19:32:34.11 delay 2 seconds
@19:32:36.13 ATV.dial :bleach
@19:32:36.77 AS.seek 0.81ml
@19:33:37.45 delay 3 seconds
@19:33:40.47 ATV.dial :air3
@19:33:41.06 delay 3 seconds
@19:33:44.13 AS.seek 0.835ml
@19:33:49.46 delay 3 seconds
@19:33:52.48 ATV.dial :bleach
@19:33:53.13 AS.seek 0.82ml
@19:33:56.54 delay 3 seconds
@19:33:59.56 ARV.dial :waste
@19:34:00.21 delay 3 seconds
@19:34:03.23 ATV.dial :ARV
@19:34:04.15 AS.reconfigure ASconfig
@19:34:04.21 AS.seek 0.47ml
@19:34:24.63 AFV.select :carrierSolution
@19:34:24.74 delay 2 seconds
@19:34:26.77 AS.seek 1ml
@19:34:57.55 delay 1 second
@19:34:58.57 AFV.select :holdingCoil
@19:34:58.67 delay 1 second
@19:34:59.70 AS.reconfigure ASfast
@19:34:59.75 AS.seek 0ml
@19:35:27.09 AFV.select :carrierSolution
@19:35:27.20 delay 2 seconds
@19:35:29.22 AS.reconfigure ASconfig
@19:35:29.28 AS.seek 1ml
@19:36:27.02 delay 1 second
@19:36:28.04 AFV.select :holdingCoil
@19:36:28.14 delay 1 second
@19:36:29.17 AS.reconfigure ASfast
@19:36:29.22 AS.seek 0ml
@19:36:56.55 AFV.select :carrierSolution
@19:36:56.66 delay 2 seconds
@19:36:58.69 AS.reconfigure ASconfig
@19:36:58.74 AS.seek 1ml
@19:37:56.52 delay 1 second
@19:37:57.54 AFV.select :holdingCoil
@19:37:57.64 delay 1 second
@19:37:58.67 AS.reconfigure ASfast
@19:37:58.72 AS.seek 0ml
@19:38:26.05 ARV.dial between carrier and air1
@19:38:26.57 ATV.dial :air3
@19:38:27.92 AS.reconfigure ASslow1
@19:38:27.98 AS.seek 0.01ml
@19:38:30.38 delay 2 seconds
@19:38:32.40 ATV.dial :bleach
@19:38:33.04 AS.seek 0.81ml
@19:41:14.21 delay 3 seconds
@19:41:17.23 ATV.dial :air3
@19:41:17.82 delay 3 seconds
@19:41:20.89 AS.seek 0.835ml
@19:41:26.19 delay 3 seconds
@19:41:29.21 ATV.dial :bleach
@19:41:29.86 AS.seek 0.82ml
@19:41:33.32 delay 3 seconds
@19:41:36.34 ATV.dial PCR
@19:41:36.77 AS.reconfigure ASconfig
@19:41:36.83 AS.seek 0ml
@19:42:24.26 PCR.program :bleachProfile
@19:42:24.32 PCR program should complete in < 2:45
@19:42:24.33 PCR.run 10:00
@19:45:20.53 PCR.off
@19:45:20.57 AFV.select :carrierSolution
@19:45:20.68 delay 2 seconds
@19:45:22.71 AS.seek 1ml
@19:46:20.46 delay 1 second
@19:46:21.48 AFV.select :holdingCoil
@19:46:21.58 delay 1 second
@19:46:22.61 AS.reconfigure ASfast
@19:46:22.66 AS.seek 0ml
@19:46:49.99 delay 3 seconds
@19:46:53.01 AFV.select :carrierSolution
@19:46:53.11 delay 2 seconds
@19:46:55.13 AS.reconfigure ASconfig
@19:46:55.20 AS.seek 1ml
@19:47:52.95 delay 1 second
@19:47:53.97 AFV.select :holdingCoil
@19:47:54.07 delay 1 second
@19:47:55.10 AS.reconfigure ASfast
@19:47:55.15 AS.seek 0ml
@19:48:22.51 delay 3 seconds
@19:48:25.53 AFV.select :carrierSolution
@19:48:25.63 delay 2 seconds
@19:48:27.66 AS.reconfigure ASconfig
@19:48:27.72 AS.seek 1ml
@19:49:25.51 delay 1 second
@19:49:26.53 AFV.select :holdingCoil
@19:49:26.63 delay 1 second
@19:49:27.66 AS.reconfigure ASfast
@19:49:27.71 AS.seek 0ml
@19:49:55.05 delay 3 seconds
@19:49:58.07 AFV.select :carrierSolution
@19:49:58.17 delay 2 seconds
@19:50:00.19 AS.reconfigure ASconfig
@19:50:00.26 AS.seek 1ml
@19:50:58.04 delay 1 second
@19:50:59.06 AFV.select :holdingCoil
@19:50:59.16 delay 1 second
@19:51:00.19 AS.reconfigure ASfast
@19:51:00.24 AS.seek 0ml
@19:51:27.59 delay 3 seconds
@19:51:30.61 AFV.select :carrierSolution
@19:51:30.71 delay 2 seconds
@19:51:32.73 AS.reconfigure ASconfig
@19:51:32.80 AS.seek 1ml
@19:52:30.60 delay 1 second
@19:52:31.62 AFV.select :holdingCoil
@19:52:31.72 delay 1 second
@19:52:32.75 AS.reconfigure ASnorm
@19:52:32.80 AS.seek 0ml
@19:53:23.53 Setting up PCR for AOPP1 -- writing /var/log/esp/AOPP121apr2119h.pcr
@19:53:23.55 Building PCR Slug from ASV with MM1 mix, PP1 primer
@19:53:23.57 Priming with carrier
@19:53:23.59 ATV.dial ARV
@19:53:24.26 ARV.dial :carrier
@19:53:24.61 AS.seek 0.5ml
@19:53:50.12 AFV.select :carrierSolution
@19:53:50.24 delay 2 seconds
@19:53:52.27 AS.reconfigure ASconfig
@19:53:52.33 AS.seek 1ml
@19:54:21.35 delay 1 second
@19:54:22.37 AFV.select :holdingCoil
@19:54:22.47 delay 1 second
@19:54:23.49 ATV.dial :waste
@19:54:25.27 AS.reconfigure ASnorm
@19:54:25.34 AS.seek 0ml
@19:55:16.07 AFV.select :carrierSolution
@19:55:16.18 delay 2 seconds
@19:55:18.21 AS.reconfigure ASconfig
@19:55:18.26 AS.seek 0.5ml
@19:55:47.29 delay 1 second
@19:55:48.31 AFV.select :holdingCoil
@19:55:48.41 Building Slug
@19:55:48.42 ATV.dial :ARV
@19:55:50.18 delay 2 seconds
@19:55:52.20 ARV.dial :air1
@19:55:52.60 delay 2 seconds
@19:55:54.67 AS.reconfigure ASslow2
@19:55:54.73 AS.seek 0.506ml
@19:56:01.23 delay 2 seconds
@19:56:03.25 ARV.dial :MM1
@19:56:03.90 delay 2 seconds
@19:56:05.92 Adding MM1
@19:56:06.00 AS.seek 0.524ml
@19:56:24.63 delay 25 seconds
@19:56:49.65 ARV.dial :PP1
@19:56:50.05 Adding PP1
@19:56:50.06 delay 2 seconds
@19:56:52.14 AS.seek 0.53ml
@19:56:58.85 delay 10 seconds
@19:57:08.88 ARV.dial :air1
@19:57:09.30 adding ARV air
@19:57:09.31 delay 2 seconds
@19:57:11.80 AS.seek 0.536ml
@19:57:18.41 delay 5 seconds
@19:57:23.43 ARV.dial :carrier
@19:57:23.87 delay 7 seconds
@19:57:30.95 AS.seek 0.594ml
@19:58:29.77 delay 5 seconds
@19:58:34.79 ATV.dial :ASV
@19:58:35.21 delay 2 seconds
@19:58:37.28 AS.seek 0.6ml
@19:58:43.80 delay 4 seconds
@19:58:47.82 ATV.dial ARV
@19:58:48.40 delay 2 seconds
@19:58:50.42 ARV.dial :carrier
@19:58:50.43 delay 2 seconds
@19:58:52.52 AS.seek 0.65ml
@19:59:43.49 delay 4 seconds
@19:59:47.51 ATV.dial PCR
@19:59:48.16 ASV.dial :waste1
@19:59:48.17 ARV.dial :air1
@19:59:48.60 Positioning Slug by 0.324ml
@19:59:48.69 AS.seek 0.326ml
@20:05:14.69 delay 5 seconds
@20:05:19.71 ATV.dial ARV
@20:05:20.54 ASV.dial :PCRlock
@20:05:20.94 delay 5 seconds
@20:05:25.96 Running AOPP1 Profile60CIQ1 thermocycling
@20:05:25.97 <PCR> PCR.program Profile60CIQ1
@20:05:26.10 Profile60CIQ1 should complete in < 1:56:15
@20:05:26.11 PCR.run 2:30:18.75
@21:45:00.58 PCR.off
@21:45:01.13 <MFBPCR> Running AOPP1 MeltProfile60 thermocycling
@21:45:01.15 <PCR> PCR.program MeltProfile60
@21:45:01.25 MeltProfile60 should complete in < 11:55
@21:45:01.26 PCR.run 19:53.75
@21:52:51.65 PCR.off
@21:52:52.57 <MFBPCR> PCR for AOPP1 completed -- closed /var/log/esp/AOPP121apr2119h.pcr
@21:52:52.64 Cleaning PCR
@21:52:52.66 AFV.select :carrierSolution
@21:52:52.77 delay 2 seconds
@21:52:54.80 AS.reconfigure ASconfig
@21:52:54.85 AS.seek 0.5ml
@21:53:05.16 delay 1 second
@21:53:06.18 AFV.select :holdingCoil
@21:53:06.28 ASV.dial :waste1
@21:53:06.68 ATV.dial :air3
@21:53:08.02 AS.reconfigure ASslow1
@21:53:08.09 AS.seek 0.51ml
@21:53:10.49 delay 2 seconds
@21:53:12.51 ATV.dial :bleach
@21:53:13.15 AS.seek 0.81ml
@21:54:13.79 delay 3 seconds
@21:54:16.81 ATV.dial :air3
@21:54:17.40 delay 3 seconds
@21:54:20.47 AS.seek 0.835ml
@21:54:25.75 delay 3 seconds
@21:54:28.77 ATV.dial :bleach
@21:54:29.42 AS.seek 0.82ml
@21:54:32.74 delay 3 seconds
@21:54:35.76 ARV.dial :waste
@21:54:36.40 delay 3 seconds
@21:54:39.42 ATV.dial :ARV
@21:54:40.34 AS.reconfigure ASconfig
@21:54:40.40 AS.seek 0.47ml
@21:55:00.82 AFV.select :carrierSolution
@21:55:00.93 delay 2 seconds
@21:55:02.96 AS.seek 1ml
@21:55:33.72 delay 1 second
@21:55:34.74 AFV.select :holdingCoil
@21:55:34.84 delay 1 second
@21:55:35.87 AS.reconfigure ASfast
@21:55:35.92 AS.seek 0ml
@21:56:03.27 AFV.select :carrierSolution
@21:56:03.38 delay 2 seconds
@21:56:05.40 AS.reconfigure ASconfig
@21:56:05.46 AS.seek 1ml
@21:57:03.21 delay 1 second
@21:57:04.23 AFV.select :holdingCoil
@21:57:04.33 delay 1 second
@21:57:05.36 AS.reconfigure ASfast
@21:57:05.41 AS.seek 0ml
@21:57:32.77 AFV.select :carrierSolution
@21:57:32.88 delay 2 seconds
@21:57:34.90 AS.reconfigure ASconfig
@21:57:34.96 AS.seek 1ml
@21:58:32.75 delay 1 second
@21:58:33.77 AFV.select :holdingCoil
@21:58:33.87 delay 1 second
@21:58:34.90 AS.reconfigure ASfast
@21:58:34.95 AS.seek 0ml
@21:59:02.27 ARV.dial between carrier and air1
@21:59:02.79 ATV.dial :air3
@21:59:04.13 AS.reconfigure ASslow1
@21:59:04.19 AS.seek 0.01ml
@21:59:06.60 delay 2 seconds
@21:59:08.62 ATV.dial :bleach
@21:59:09.26 AS.seek 0.81ml
@22:01:50.43 delay 3 seconds
@22:01:53.45 ATV.dial :air3
@22:01:54.04 delay 3 seconds
@22:01:57.52 AS.seek 0.835ml
@22:02:02.80 delay 3 seconds
@22:02:05.82 ATV.dial :bleach
@22:02:06.47 AS.seek 0.82ml
@22:02:09.77 delay 3 seconds
@22:02:12.79 ATV.dial PCR
@22:02:13.21 AS.reconfigure ASconfig
@22:02:13.27 AS.seek 0ml
@22:03:00.69 PCR.program :bleachProfile
@22:03:00.76 PCR program should complete in < 2:45
@22:03:00.77 PCR.run 10:00
@22:05:56.56 PCR.off
@22:05:56.60 AFV.select :carrierSolution
@22:05:56.71 delay 2 seconds
@22:05:58.74 AS.seek 1ml
@22:06:56.49 delay 1 second
@22:06:57.51 AFV.select :holdingCoil
@22:06:57.61 delay 1 second
@22:06:58.64 AS.reconfigure ASfast
@22:06:58.69 AS.seek 0ml
@22:07:26.05 delay 3 seconds
@22:07:29.07 AFV.select :carrierSolution
@22:07:29.19 delay 2 seconds
@22:07:31.22 AS.reconfigure ASconfig
@22:07:31.27 AS.seek 1ml
@22:08:29.07 delay 1 second
@22:08:30.09 AFV.select :holdingCoil
@22:08:30.19 delay 1 second
@22:08:31.22 AS.reconfigure ASfast
@22:08:31.27 AS.seek 0ml
@22:08:58.61 delay 3 seconds
@22:09:01.63 AFV.select :carrierSolution
@22:09:01.73 delay 2 seconds
@22:09:03.76 AS.reconfigure ASconfig
@22:09:03.82 AS.seek 1ml
@22:10:01.55 delay 1 second
@22:10:02.57 AFV.select :holdingCoil
@22:10:02.67 delay 1 second
@22:10:03.70 AS.reconfigure ASfast
@22:10:03.75 AS.seek 0ml
@22:10:31.08 delay 3 seconds
@22:10:34.10 AFV.select :carrierSolution
@22:10:34.20 delay 2 seconds
@22:10:36.22 AS.reconfigure ASconfig
@22:10:36.29 AS.seek 1ml
@22:11:34.04 delay 1 second
@22:11:35.06 AFV.select :holdingCoil
@22:11:35.16 delay 1 second
@22:11:36.19 AS.reconfigure ASfast
@22:11:36.24 AS.seek 0ml
@22:12:03.58 delay 3 seconds
@22:12:06.60 AFV.select :carrierSolution
@22:12:06.70 delay 2 seconds
@22:12:08.72 AS.reconfigure ASconfig
@22:12:08.79 AS.seek 1ml
@22:13:06.54 delay 1 second
@22:13:07.56 AFV.select :holdingCoil
@22:13:07.66 delay 1 second
@22:13:08.69 AS.reconfigure ASnorm
@22:13:08.74 AS.seek 0ml
@22:13:59.41 Setting up PCR for AARPP4 -- writing /var/log/esp/AARPP421apr2122h.pcr
@22:13:59.43 Building PCR Slug from ASV with MM2 mix, PP4 primer
@22:13:59.45 Priming with carrier
@22:13:59.47 ATV.dial ARV
@22:14:00.13 ARV.dial :carrier
@22:14:00.49 AS.seek 0.5ml
@22:14:26.00 AFV.select :carrierSolution
@22:14:26.11 delay 2 seconds
@22:14:28.52 AS.reconfigure ASconfig
@22:14:28.60 AS.seek 1ml
@22:14:57.61 delay 1 second
@22:14:58.63 AFV.select :holdingCoil
@22:14:58.73 delay 1 second
@22:14:59.75 ATV.dial :waste
@22:15:01.54 AS.reconfigure ASnorm
@22:15:01.60 AS.seek 0ml
@22:15:52.33 AFV.select :carrierSolution
@22:15:52.45 delay 2 seconds
@22:15:54.48 AS.reconfigure ASconfig
@22:15:54.53 AS.seek 0.5ml
@22:16:23.58 delay 1 second
@22:16:24.60 AFV.select :holdingCoil
@22:16:24.70 Building Slug
@22:16:24.72 ATV.dial :ARV
@22:16:26.57 delay 2 seconds
@22:16:28.59 ARV.dial :air1
@22:16:29.00 delay 2 seconds
@22:16:31.08 AS.reconfigure ASslow2
@22:16:31.14 AS.seek 0.506ml
@22:16:37.39 delay 2 seconds
@22:16:39.41 ARV.dial :MM2
@22:16:41.02 delay 2 seconds
@22:16:43.04 Adding MM2
@22:16:43.12 AS.seek 0.524ml
@22:17:01.66 delay 25 seconds
@22:17:26.68 ARV.dial :PP4
@22:17:27.10 Adding PP4
@22:17:27.11 delay 2 seconds
@22:17:29.20 AS.seek 0.53ml
@22:17:35.71 delay 10 seconds
@22:17:45.74 ARV.dial :air3
@22:17:46.16 adding ARV air
@22:17:46.17 delay 2 seconds
@22:17:48.25 AS.seek 0.536ml
@22:17:54.60 delay 5 seconds
@22:17:59.62 ARV.dial :carrier
@22:18:00.96 delay 7 seconds
@22:18:08.04 AS.seek 0.594ml
@22:19:06.69 delay 5 seconds
@22:19:11.71 ATV.dial :ASV
@22:19:12.15 delay 2 seconds
@22:19:14.22 AS.seek 0.6ml
@22:19:20.88 delay 4 seconds
@22:19:24.90 ATV.dial ARV
@22:19:25.49 delay 2 seconds
@22:19:27.51 ARV.dial :carrier
@22:19:27.52 delay 2 seconds
@22:19:29.60 AS.seek 0.65ml
@22:20:20.54 delay 4 seconds
@22:20:24.56 ATV.dial PCR
@22:20:25.21 ASV.dial :waste1
@22:20:25.22 ARV.dial :air1
@22:20:25.64 Positioning Slug by 0.324ml
@22:20:25.73 AS.seek 0.326ml
@22:25:51.75 delay 5 seconds
@22:25:56.77 ATV.dial ARV
@22:25:57.60 ASV.dial :PCRlock
@22:25:58.00 delay 5 seconds
@22:26:03.02 Running AARPP4 Profile58CIQ2 thermocycling
@22:26:03.03 <PCR> PCR.program Profile58CIQ2
@22:26:03.15 Profile58CIQ2 should complete in < 1:36:15
@22:26:03.16 PCR.run 2:05:18.75
@23:47:24.61 PCR.off
@23:47:25.16 <MFBPCR> Running AARPP4 MeltProfile58 thermocycling
@23:47:25.18 <PCR> PCR.program MeltProfile58
@23:47:25.28 MeltProfile58 should complete in < 12:33
@23:47:25.29 PCR.run 20:41.25
@23:55:15.42 PCR.off
@23:55:16.40 <MFBPCR> PCR for AARPP4 completed -- closed /var/log/esp/AARPP421apr2122h.pcr
@23:55:16.47 Cleaning PCR
@23:55:16.48 AFV.select :carrierSolution
@23:55:16.60 delay 2 seconds
@23:55:18.63 AS.reconfigure ASconfig
@23:55:18.68 AS.seek 0.5ml
@23:55:28.96 delay 1 second
@23:55:29.98 AFV.select :holdingCoil
@23:55:30.08 ASV.dial :waste1
@23:55:30.49 ATV.dial :air3
@23:55:31.85 AS.reconfigure ASslow1
@23:55:31.91 AS.seek 0.51ml
@23:55:34.21 delay 2 seconds
@23:55:36.24 ATV.dial :bleach
@23:55:37.27 AS.seek 0.81ml
@23:56:37.88 delay 3 seconds
@23:56:40.90 ATV.dial :air3
@23:56:41.49 delay 3 seconds
@23:56:44.56 AS.seek 0.835ml
@23:56:49.84 delay 3 seconds
@23:56:52.86 ATV.dial :bleach
@23:56:53.52 AS.seek 0.82ml
@23:56:56.92 delay 3 seconds
@23:56:59.94 ARV.dial :waste
@23:57:00.58 delay 3 seconds
@23:57:03.60 ATV.dial :ARV
@23:57:04.55 AS.reconfigure ASconfig
@23:57:04.60 AS.seek 0.47ml
@23:57:25.03 AFV.select :carrierSolution
@23:57:25.14 delay 2 seconds
@23:57:27.17 AS.seek 1ml
@23:57:57.91 delay 1 second
@23:57:58.93 AFV.select :holdingCoil
@23:57:59.03 delay 1 second
@23:58:00.06 AS.reconfigure ASfast
@23:58:00.11 AS.seek 0ml
@23:58:27.46 AFV.select :carrierSolution
@23:58:27.57 delay 2 seconds
@23:58:29.60 AS.reconfigure ASconfig
@23:58:29.65 AS.seek 1ml
@23:59:27.44 delay 1 second
@23:59:28.46 AFV.select :holdingCoil
@23:59:28.56 delay 1 second
@23:59:29.59 AS.reconfigure ASfast
@23:59:29.64 AS.seek 0ml
@23:59:56.97 AFV.select :carrierSolution
@23:59:57.08 delay 2 seconds
@23:59:59.11 AS.reconfigure ASconfig
@23:59:59.17 AS.seek 1ml
@00:00:56.91CEST22-Apr-21 delay 1 second
@00:00:57.93 AFV.select :holdingCoil
@00:00:58.03 delay 1 second
@00:00:59.06 AS.reconfigure ASfast
@00:00:59.11 AS.seek 0ml
@00:01:26.44 ARV.dial between carrier and air1
@00:01:26.96 ATV.dial :air3
@00:01:28.30 AS.reconfigure ASslow1
@00:01:28.36 AS.seek 0.01ml
@00:01:30.78 delay 2 seconds
@00:01:32.80 ATV.dial :bleach
@00:01:33.45 AS.seek 0.81ml
@00:04:14.60 delay 3 seconds
@00:04:17.62 ATV.dial :air3
@00:04:18.21 delay 3 seconds
@00:04:21.28 AS.seek 0.835ml
@00:04:26.55 delay 3 seconds
@00:04:29.57 ATV.dial :bleach
@00:04:30.21 AS.seek 0.82ml
@00:04:33.52 delay 3 seconds
@00:04:36.54 ATV.dial PCR
@00:04:36.97 AS.reconfigure ASconfig
@00:04:37.03 AS.seek 0ml
@00:05:24.44 PCR.program :bleachProfile
@00:05:24.51 PCR program should complete in < 2:45
@00:05:24.52 PCR.run 10:00
@00:08:20.72 PCR.off
@00:08:20.76 AFV.select :carrierSolution
@00:08:20.87 delay 2 seconds
@00:08:22.90 AS.seek 1ml
@00:09:20.64 delay 1 second
@00:09:21.66 AFV.select :holdingCoil
@00:09:21.76 delay 1 second
@00:09:22.79 AS.reconfigure ASfast
@00:09:22.84 AS.seek 0ml
@00:09:50.17 delay 3 seconds
@00:09:53.19 AFV.select :carrierSolution
@00:09:53.29 delay 2 seconds
@00:09:55.32 AS.reconfigure ASconfig
@00:09:55.38 AS.seek 1ml
@00:10:53.16 delay 1 second
@00:10:54.18 AFV.select :holdingCoil
@00:10:54.28 delay 1 second
@00:10:55.31 AS.reconfigure ASfast
@00:10:55.36 AS.seek 0ml
@00:11:22.71 delay 3 seconds
@00:11:25.73 AFV.select :carrierSolution
@00:11:25.83 delay 2 seconds
@00:11:27.86 AS.reconfigure ASconfig
@00:11:27.92 AS.seek 1ml
@00:12:25.66 delay 1 second
@00:12:26.68 AFV.select :holdingCoil
@00:12:26.78 delay 1 second
@00:12:27.81 AS.reconfigure ASfast
@00:12:27.86 AS.seek 0ml
@00:12:55.21 delay 3 seconds
@00:12:58.23 AFV.select :carrierSolution
@00:12:58.33 delay 2 seconds
@00:13:00.36 AS.reconfigure ASconfig
@00:13:00.42 AS.seek 1ml
@00:13:58.16 delay 1 second
@00:13:59.18 AFV.select :holdingCoil
@00:13:59.28 delay 1 second
@00:14:00.31 AS.reconfigure ASfast
@00:14:00.36 AS.seek 0ml
@00:14:27.71 delay 3 seconds
@00:14:30.73 AFV.select :carrierSolution
@00:14:30.83 delay 2 seconds
@00:14:32.85 AS.reconfigure ASconfig
@00:14:32.92 AS.seek 1ml
@00:15:30.71 delay 1 second
@00:15:31.73 AFV.select :holdingCoil
@00:15:31.83 delay 1 second
@00:15:32.86 AS.reconfigure ASnorm
@00:15:32.91 AS.seek 0ml
@00:16:23.62 Setting up PCR for AALPP3 -- writing /var/log/esp/AALPP321apr2200h.pcr
@00:16:23.64 Building PCR Slug from ASV with MM2 mix, PP3 primer
@00:16:23.66 Priming with carrier
@00:16:23.68 ATV.dial ARV
@00:16:24.35 ARV.dial :carrier
@00:16:24.70 AS.seek 0.5ml
@00:16:50.30 AFV.select :carrierSolution
@00:16:50.42 delay 2 seconds
@00:16:52.45 AS.reconfigure ASconfig
@00:16:52.51 AS.seek 1ml
@00:17:21.52 delay 1 second
@00:17:22.54 AFV.select :holdingCoil
@00:17:22.64 delay 1 second
@00:17:23.66 ATV.dial :waste
@00:17:25.44 AS.reconfigure ASnorm
@00:17:25.50 AS.seek 0ml
@00:18:16.19 AFV.select :carrierSolution
@00:18:16.30 delay 2 seconds
@00:18:18.33 AS.reconfigure ASconfig
@00:18:18.38 AS.seek 0.5ml
@00:18:47.44 delay 1 second
@00:18:48.46 AFV.select :holdingCoil
@00:18:48.56 Building Slug
@00:18:48.57 ATV.dial :ARV
@00:18:50.33 delay 2 seconds
@00:18:52.35 ARV.dial :air1
@00:18:52.75 delay 2 seconds
@00:18:54.82 AS.reconfigure ASslow2
@00:18:54.88 AS.seek 0.506ml
@00:19:01.06 delay 2 seconds
@00:19:03.08 ARV.dial :MM2
@00:19:04.67 delay 2 seconds
@00:19:06.69 Adding MM2
@00:19:06.77 AS.seek 0.524ml
@00:19:25.19 delay 25 seconds
@00:19:50.21 ARV.dial :PP3
@00:19:50.61 Adding PP3
@00:19:50.62 delay 2 seconds
@00:19:52.70 AS.seek 0.53ml
@00:19:59.36 delay 10 seconds
@00:20:09.39 ARV.dial :air2
@00:20:09.83 adding ARV air
@00:20:09.84 delay 2 seconds
@00:20:11.92 AS.seek 0.536ml
@00:20:18.53 delay 5 seconds
@00:20:23.55 ARV.dial :carrier
@00:20:24.89 delay 7 seconds
@00:20:31.97 AS.seek 0.594ml
@00:21:30.44 delay 5 seconds
@00:21:35.46 ATV.dial :ASV
@00:21:35.88 delay 2 seconds
@00:21:37.95 AS.seek 0.6ml
@00:21:44.27 delay 4 seconds
@00:21:48.29 ATV.dial ARV
@00:21:48.86 delay 2 seconds
@00:21:50.88 ARV.dial :carrier
@00:21:50.89 delay 2 seconds
@00:21:52.97 AS.seek 0.65ml
@00:22:43.95 delay 4 seconds
@00:22:47.97 ATV.dial PCR
@00:22:48.63 ASV.dial :waste1
@00:22:48.64 ARV.dial :air1
@00:22:49.06 Positioning Slug by 0.324ml
@00:22:49.14 AS.seek 0.326ml
@00:28:15.44 delay 5 seconds
@00:28:20.46 ATV.dial ARV
@00:28:21.27 ASV.dial :PCRlock
@00:28:21.67 delay 5 seconds
@00:28:26.69 Running AALPP3 Profile58CIQ2 thermocycling
@00:28:26.70 <PCR> PCR.program Profile58CIQ2
@00:28:27.23 Profile58CIQ2 should complete in < 1:36:15
@00:28:27.25 PCR.run 2:05:18.75
@01:49:41.06 PCR.off
@01:49:41.61 <MFBPCR> Running AALPP3 MeltProfile58 thermocycling
@01:49:41.63 <PCR> PCR.program MeltProfile58
@01:49:42.09 MeltProfile58 should complete in < 12:33
@01:49:42.12 PCR.run 20:41.25
@01:57:42.31 PCR.off
@01:57:43.29 <MFBPCR> PCR for AALPP3 completed -- closed /var/log/esp/AALPP321apr2200h.pcr
@01:57:43.35 Cleaning PCR
@01:57:43.37 AFV.select :carrierSolution
@01:57:43.49 delay 2 seconds
@01:57:45.52 AS.reconfigure ASconfig
@01:57:45.57 AS.seek 0.5ml
@01:57:55.91 delay 1 second
@01:57:56.93 AFV.select :holdingCoil
@01:57:57.03 ASV.dial :waste1
@01:57:57.42 ATV.dial :air3
@01:57:58.78 AS.reconfigure ASslow1
@01:57:58.84 AS.seek 0.51ml
@01:58:01.14 delay 2 seconds
@01:58:03.16 ATV.dial :bleach
@01:58:03.81 AS.seek 0.81ml
@01:59:04.53 delay 3 seconds
@01:59:07.55 ATV.dial :air3
@01:59:08.14 delay 3 seconds
@01:59:11.21 AS.seek 0.835ml
@01:59:16.50 delay 3 seconds
@01:59:19.52 ATV.dial :bleach
@01:59:20.17 AS.seek 0.82ml
@01:59:23.61 delay 3 seconds
@01:59:26.63 ARV.dial :waste
@01:59:27.27 delay 3 seconds
@01:59:30.29 ATV.dial :ARV
@01:59:31.22 AS.reconfigure ASconfig
@01:59:31.28 AS.seek 0.47ml
@01:59:51.66 AFV.select :carrierSolution
@01:59:51.76 delay 2 seconds
@01:59:53.79 AS.seek 1ml
@02:00:24.53 delay 1 second
@02:00:25.55 AFV.select :holdingCoil
@02:00:25.65 delay 1 second
@02:00:26.68 AS.reconfigure ASfast
@02:00:26.73 AS.seek 0ml
@02:00:54.09 AFV.select :carrierSolution
@02:00:54.20 delay 2 seconds
@02:00:56.23 AS.reconfigure ASconfig
@02:00:56.28 AS.seek 1ml
@02:01:54.02 delay 1 second
@02:01:55.04 AFV.select :holdingCoil
@02:01:55.14 delay 1 second
@02:01:56.17 AS.reconfigure ASfast
@02:01:56.22 AS.seek 0ml
@02:02:23.56 AFV.select :carrierSolution
@02:02:23.67 delay 2 seconds
@02:02:25.69 AS.reconfigure ASconfig
@02:02:25.75 AS.seek 1ml
@02:03:23.50 delay 1 second
@02:03:24.52 AFV.select :holdingCoil
@02:03:24.62 delay 1 second
@02:03:25.65 AS.reconfigure ASfast
@02:03:25.70 AS.seek 0ml
@02:03:53.03 ARV.dial between carrier and air1
@02:03:53.56 ATV.dial :air3
@02:03:54.93 AS.reconfigure ASslow1
@02:03:54.99 AS.seek 0.01ml
@02:03:57.38 delay 2 seconds
@02:03:59.40 ATV.dial :bleach
@02:04:00.06 AS.seek 0.81ml
@02:06:41.31 delay 3 seconds
@02:06:44.33 ATV.dial :air3
@02:06:44.92 delay 3 seconds
@02:06:47.99 AS.seek 0.835ml
@02:06:53.28 delay 3 seconds
@02:06:56.30 ATV.dial :bleach
@02:06:56.94 AS.seek 0.82ml
@02:07:00.36 delay 3 seconds
@02:07:03.38 ATV.dial PCR
@02:07:03.81 AS.reconfigure ASconfig
@02:07:03.87 AS.seek 0ml
@02:07:51.30 PCR.program :bleachProfile
@02:07:51.37 PCR program should complete in < 2:45
@02:07:51.38 PCR.run 10:00
@02:10:47.74 PCR.off
@02:10:47.78 AFV.select :carrierSolution
@02:10:47.89 delay 2 seconds
@02:10:49.92 AS.seek 1ml
@02:11:47.67 delay 1 second
@02:11:48.69 AFV.select :holdingCoil
@02:11:48.79 delay 1 second
@02:11:49.82 AS.reconfigure ASfast
@02:11:49.87 AS.seek 0ml
@02:12:17.22 delay 3 seconds
@02:12:20.24 AFV.select :carrierSolution
@02:12:20.34 delay 2 seconds
@02:12:22.37 AS.reconfigure ASconfig
@02:12:22.43 AS.seek 1ml
@02:13:20.17 delay 1 second
@02:13:21.19 AFV.select :holdingCoil
@02:13:21.29 delay 1 second
@02:13:22.32 AS.reconfigure ASfast
@02:13:22.37 AS.seek 0ml
@02:13:49.72 delay 3 seconds
@02:13:52.74 AFV.select :carrierSolution
@02:13:52.84 delay 2 seconds
@02:13:54.86 AS.reconfigure ASconfig
@02:13:54.93 AS.seek 1ml
@02:14:52.67 delay 1 second
@02:14:53.69 AFV.select :holdingCoil
@02:14:53.79 delay 1 second
@02:14:54.82 AS.reconfigure ASfast
@02:14:54.87 AS.seek 0ml
@02:15:22.22 delay 3 seconds
@02:15:25.24 AFV.select :carrierSolution
@02:15:25.34 delay 2 seconds
@02:15:27.36 AS.reconfigure ASconfig
@02:15:27.43 AS.seek 1ml
@02:16:25.19 delay 1 second
@02:16:26.21 AFV.select :holdingCoil
@02:16:26.31 delay 1 second
@02:16:27.34 AS.reconfigure ASfast
@02:16:27.39 AS.seek 0ml
@02:16:54.73 delay 3 seconds
@02:16:57.75 AFV.select :carrierSolution
@02:16:57.85 delay 2 seconds
@02:16:59.87 AS.reconfigure ASconfig
@02:16:59.94 AS.seek 1ml
@02:17:57.73 delay 1 second
@02:17:58.75 AFV.select :holdingCoil
@02:17:58.85 delay 1 second
@02:17:59.88 AS.reconfigure ASnorm
@02:17:59.93 AS.seek 0ml
@02:18:50.63 Setting up PCR for ACOPP5 -- writing /var/log/esp/ACOPP521apr2202h.pcr
@02:18:50.65 Building PCR Slug from ASV with MM3 mix, PP5 primer
@02:18:50.67 Priming with carrier
@02:18:50.69 ATV.dial ARV
@02:18:51.34 ARV.dial :carrier
@02:18:51.69 AS.seek 0.5ml
@02:19:17.28 AFV.select :carrierSolution
@02:19:17.40 delay 2 seconds
@02:19:19.43 AS.reconfigure ASconfig
@02:19:19.49 AS.seek 1ml
@02:19:48.53 delay 1 second
@02:19:49.55 AFV.select :holdingCoil
@02:19:49.65 delay 1 second
@02:19:50.67 ATV.dial :waste
@02:19:52.45 AS.reconfigure ASnorm
@02:19:52.52 AS.seek 0ml
@02:20:43.20 AFV.select :carrierSolution
@02:20:43.32 delay 2 seconds
@02:20:45.35 AS.reconfigure ASconfig
@02:20:45.40 AS.seek 0.5ml
@02:21:14.47 delay 1 second
@02:21:15.49 AFV.select :holdingCoil
@02:21:15.59 Building Slug
@02:21:15.60 ATV.dial :ARV
@02:21:17.36 delay 2 seconds
@02:21:19.38 ARV.dial :air1
@02:21:19.87 delay 2 seconds
@02:21:21.94 AS.reconfigure ASslow2
@02:21:22.00 AS.seek 0.506ml
@02:21:28.94 delay 2 seconds
@02:21:30.96 ARV.dial :MM3
@02:21:32.06 delay 2 seconds
@02:21:34.08 Adding MM3
@02:21:34.17 AS.seek 0.524ml
@02:21:52.75 delay 25 seconds
@02:22:17.77 ARV.dial :PP5
@02:22:18.19 Adding PP5
@02:22:18.20 delay 2 seconds
@02:22:20.28 AS.seek 0.53ml
@02:22:26.84 delay 10 seconds
@02:22:36.87 ARV.dial :air3
@02:22:37.30 adding ARV air
@02:22:37.31 delay 2 seconds
@02:22:39.39 AS.seek 0.536ml
@02:22:45.67 delay 5 seconds
@02:22:50.69 ARV.dial :carrier
@02:22:52.05 delay 7 seconds
@02:22:59.13 AS.seek 0.594ml
@02:23:57.94 delay 5 seconds
@02:24:02.96 ATV.dial :ASV
@02:24:03.39 delay 2 seconds
@02:24:05.46 AS.seek 0.6ml
@02:24:11.67 delay 4 seconds
@02:24:15.69 ATV.dial ARV
@02:24:16.28 delay 2 seconds
@02:24:18.30 ARV.dial :carrier
@02:24:18.31 delay 2 seconds
@02:24:20.39 AS.seek 0.65ml
@02:25:11.16 delay 4 seconds
@02:25:15.18 ATV.dial PCR
@02:25:15.83 ASV.dial :waste1
@02:25:15.84 ARV.dial :air1
@02:25:16.28 Positioning Slug by 0.324ml
@02:25:16.37 AS.seek 0.326ml
@02:30:42.31 delay 5 seconds
@02:30:47.33 ATV.dial ARV
@02:30:48.14 ASV.dial :PCRlock
@02:30:48.55 delay 5 seconds
@02:30:53.57 Running ACOPP5 ProfileIPc thermocycling
@02:30:53.58 <PCR> PCR.program ProfileIPc
@02:30:53.70 ProfileIPc should complete in < 1:26:35
@02:30:53.71 PCR.run 1:53:13.75
@03:39:52.39 PCR.off
@03:39:52.96 <MFBPCR> PCR for ACOPP5 completed -- closed /var/log/esp/ACOPP521apr2202h.pcr
@03:39:53.03 Cleaning PCR
@03:39:53.04 AFV.select :carrierSolution
@03:39:53.16 delay 2 seconds
@03:39:55.19 AS.reconfigure ASconfig
@03:39:55.24 AS.seek 0.5ml
@03:40:05.56 delay 1 second
@03:40:06.58 AFV.select :holdingCoil
@03:40:06.68 ASV.dial :waste1
@03:40:07.08 ATV.dial :air3
@03:40:08.44 AS.reconfigure ASslow1
@03:40:08.51 AS.seek 0.51ml
@03:40:10.79 delay 2 seconds
@03:40:12.81 ATV.dial :bleach
@03:40:13.47 AS.seek 0.81ml
@03:41:14.05 delay 3 seconds
@03:41:17.07 ATV.dial :air3
@03:41:17.66 delay 3 seconds
@03:41:20.73 AS.seek 0.835ml
@03:41:26.06 delay 3 seconds
@03:41:29.08 ATV.dial :bleach
@03:41:29.73 AS.seek 0.82ml
@03:41:32.98 delay 3 seconds
@03:41:36.00 ARV.dial :waste
@03:41:36.66 delay 3 seconds
@03:41:39.68 ATV.dial :ARV
@03:41:40.60 AS.reconfigure ASconfig
@03:41:40.66 AS.seek 0.47ml
@03:42:01.06 AFV.select :carrierSolution
@03:42:01.17 delay 2 seconds
@03:42:03.20 AS.seek 1ml
@03:42:33.95 delay 1 second
@03:42:34.97 AFV.select :holdingCoil
@03:42:35.07 delay 1 second
@03:42:36.10 AS.reconfigure ASfast
@03:42:36.15 AS.seek 0ml
@03:43:03.51 AFV.select :carrierSolution
@03:43:03.62 delay 2 seconds
@03:43:05.64 AS.reconfigure ASconfig
@03:43:05.70 AS.seek 1ml
@03:44:03.45 delay 1 second
@03:44:04.47 AFV.select :holdingCoil
@03:44:04.57 delay 1 second
@03:44:05.60 AS.reconfigure ASfast
@03:44:05.65 AS.seek 0ml
@03:44:32.98 AFV.select :carrierSolution
@03:44:33.09 delay 2 seconds
@03:44:35.11 AS.reconfigure ASconfig
@03:44:35.17 AS.seek 1ml
@03:45:32.91 delay 1 second
@03:45:33.93 AFV.select :holdingCoil
@03:45:34.03 delay 1 second
@03:45:35.06 AS.reconfigure ASfast
@03:45:35.11 AS.seek 0ml
@03:46:02.45 ARV.dial between carrier and air1
@03:46:02.98 ATV.dial :air3
@03:46:04.34 AS.reconfigure ASslow1
@03:46:04.40 AS.seek 0.01ml
@03:46:06.70 delay 2 seconds
@03:46:08.72 ATV.dial :bleach
@03:46:09.37 AS.seek 0.81ml
@03:48:50.51 delay 3 seconds
@03:48:53.53 ATV.dial :air3
@03:48:54.12 delay 3 seconds
@03:48:57.19 AS.seek 0.835ml
@03:49:02.87 delay 3 seconds
@03:49:05.89 ATV.dial :bleach
@03:49:06.54 AS.seek 0.82ml
@03:49:09.97 delay 3 seconds
@03:49:12.99 ATV.dial PCR
@03:49:13.42 AS.reconfigure ASconfig
@03:49:13.48 AS.seek 0ml
@03:50:00.91 PCR.program :bleachProfile
@03:50:00.98 PCR program should complete in < 2:45
@03:50:00.99 PCR.run 10:00
@03:52:56.78 PCR.off
@03:52:56.82 AFV.select :carrierSolution
@03:52:56.93 delay 2 seconds
@03:52:58.96 AS.seek 1ml
@03:53:56.75 delay 1 second
@03:53:57.77 AFV.select :holdingCoil
@03:53:57.87 delay 1 second
@03:53:58.90 AS.reconfigure ASfast
@03:53:58.95 AS.seek 0ml
@03:54:26.26 delay 3 seconds
@03:54:29.28 AFV.select :carrierSolution
@03:54:29.40 delay 2 seconds
@03:54:31.43 AS.reconfigure ASconfig
@03:54:31.49 AS.seek 1ml
@03:55:29.23 delay 1 second
@03:55:30.25 AFV.select :holdingCoil
@03:55:30.35 delay 1 second
@03:55:31.38 AS.reconfigure ASfast
@03:55:31.43 AS.seek 0ml
@03:55:58.76 delay 3 seconds
@03:56:01.78 AFV.select :carrierSolution
@03:56:01.88 delay 2 seconds
@03:56:03.90 AS.reconfigure ASconfig
@03:56:03.97 AS.seek 1ml
@03:57:01.72 delay 1 second
@03:57:02.74 AFV.select :holdingCoil
@03:57:02.84 delay 1 second
@03:57:03.87 AS.reconfigure ASfast
@03:57:03.92 AS.seek 0ml
@03:57:31.26 delay 3 seconds
@03:57:34.28 AFV.select :carrierSolution
@03:57:34.39 delay 2 seconds
@03:57:36.41 AS.reconfigure ASconfig
@03:57:36.48 AS.seek 1ml
@03:58:34.20 delay 1 second
@03:58:35.22 AFV.select :holdingCoil
@03:58:35.32 delay 1 second
@03:58:36.35 AS.reconfigure ASfast
@03:58:36.40 AS.seek 0ml
@03:59:03.76 delay 3 seconds
@03:59:06.78 AFV.select :carrierSolution
@03:59:06.91 delay 2 seconds
@03:59:08.94 AS.reconfigure ASconfig
@03:59:09.00 AS.seek 1ml
@04:00:06.78 delay 1 second
@04:00:07.80 AFV.select :holdingCoil
@04:00:07.90 delay 1 second
@04:00:08.92 AS.reconfigure ASnorm
@04:00:08.98 AS.seek 0ml
@04:00:59.69 Setting up PCR for AIPc -- writing /var/log/esp/AIPc21apr2204h.pcr
@04:00:59.71 Building PCR Slug from ASV with MM3 mix, PP6 primer
@04:00:59.73 Priming with carrier
@04:00:59.75 ATV.dial ARV
@04:01:00.41 ARV.dial :carrier
@04:01:00.76 AS.seek 0.5ml
@04:01:26.28 AFV.select :carrierSolution
@04:01:26.40 delay 2 seconds
@04:01:28.43 AS.reconfigure ASconfig
@04:01:28.49 AS.seek 1ml
@04:01:57.94 delay 1 second
@04:01:58.96 AFV.select :holdingCoil
@04:01:59.06 delay 1 second
@04:02:00.08 ATV.dial :waste
@04:02:01.86 AS.reconfigure ASnorm
@04:02:01.93 AS.seek 0ml
@04:02:52.61 AFV.select :carrierSolution
@04:02:52.72 delay 2 seconds
@04:02:54.75 AS.reconfigure ASconfig
@04:02:54.80 AS.seek 0.5ml
@04:03:23.86 delay 1 second
@04:03:24.88 AFV.select :holdingCoil
@04:03:24.98 Building Slug
@04:03:24.99 ATV.dial :ARV
@04:03:26.75 delay 2 seconds
@04:03:28.77 ARV.dial :air1
@04:03:29.18 delay 2 seconds
@04:03:31.25 AS.reconfigure ASslow2
@04:03:31.31 AS.seek 0.506ml
@04:03:37.50 delay 2 seconds
@04:03:39.52 ARV.dial :MM3
@04:03:40.62 delay 2 seconds
@04:03:42.64 Adding MM3
@04:03:42.73 AS.seek 0.524ml
@04:04:01.31 delay 25 seconds
@04:04:26.33 ARV.dial :PP6
@04:04:26.73 Adding PP6
@04:04:26.74 delay 2 seconds
@04:04:28.82 AS.seek 0.53ml
@04:04:35.40 delay 10 seconds
@04:04:45.43 ARV.dial :air1
@04:04:46.31 adding ARV air
@04:04:46.32 delay 2 seconds
@04:04:48.40 AS.seek 0.536ml
@04:04:55.06 delay 5 seconds
@04:05:00.08 ARV.dial :carrier
@04:05:00.50 delay 7 seconds
@04:05:07.58 AS.seek 0.594ml
@04:06:06.09 delay 5 seconds
@04:06:11.11 ATV.dial :ASV
@04:06:11.53 delay 2 seconds
@04:06:13.60 AS.seek 0.6ml
@04:06:20.25 delay 4 seconds
@04:06:24.27 ATV.dial ARV
@04:06:24.84 delay 2 seconds
@04:06:26.86 ARV.dial :carrier
@04:06:26.87 delay 2 seconds
@04:06:28.95 AS.seek 0.65ml
@04:07:19.59 delay 4 seconds
@04:07:23.61 ATV.dial PCR
@04:07:24.26 ASV.dial :waste1
@04:07:24.27 ARV.dial :air1
@04:07:24.70 Positioning Slug by 0.324ml
@04:07:24.79 AS.seek 0.326ml
@04:12:51.33 delay 5 seconds
@04:12:56.35 ATV.dial ARV
@04:12:57.17 ASV.dial :PCRlock
@04:12:57.58 delay 5 seconds
@04:13:02.60 Running AIPc ProfileIPc thermocycling
@04:13:02.61 <PCR> PCR.program ProfileIPc
@04:13:02.73 ProfileIPc should complete in < 1:26:35
@04:13:02.74 PCR.run 1:53:13.75
@05:21:58.71 PCR.off
@05:21:59.28 <MFBPCR> PCR for AIPc completed -- closed /var/log/esp/AIPc21apr2204h.pcr
@05:21:59.29 Full MFB Decontamination
@05:21:59.31 Decon extraction column and decon extSrc
@05:21:59.34 SPE.hold 2:46:40,55C
@05:21:59.38 ASV.dial :SPEdelivery
@05:22:00.08 delay 20 seconds
@05:22:20.11 AFV.select :carrierSolution
@05:22:20.21 delay 2 seconds
@05:22:22.24 AS.reconfigure ASconfig
@05:22:22.29 AS.seek 0.5ml
@05:22:32.61 delay 1 second
@05:22:33.63 AFV.select :holdingCoil
@05:22:33.73 ATV.dial :air1
@05:22:35.79 AS.reconfigure ASslow1
@05:22:35.85 AS.seek 0.52ml
@05:22:40.20 ATV.dial :ASV
@05:22:42.15 ASV.dial :SPErecovery
@05:22:42.84 AS.seek 0.62ml
@05:23:03.25 ATV.dial :waste
@05:23:05.47 AS.reconfigure ASnorm
@05:23:05.53 AS.seek 0ml
@05:23:37.12 deconEluateRecoveryLine extSrc
@05:23:37.13 ATV.dial :waste
@05:23:37.16 AS.seek 0ml
@05:23:37.24 ATV.dial :air3
@05:23:38.01 AS.reconfigure ASslow1
@05:23:38.07 AS.seek 0.01ml
@05:23:40.39 delay 2 seconds
@05:23:42.41 ATV.dial :bleach
@05:23:42.93 AS.seek 0.31ml
@05:24:43.50 delay 3 seconds
@05:24:46.52 ATV.dial :air3
@05:24:47.15 delay 3 seconds
@05:24:50.22 AS.seek 0.335ml
@05:24:55.67 delay 3 seconds
@05:24:58.69 ATV.dial :bleach
@05:24:59.37 AS.seek 0.32ml
@05:25:02.67 delay 3 seconds
@05:25:05.69 ATV.dial :extSrc
@05:25:06.37 AS.seek 0ml
@05:26:11.00 AFV.select :carrierSolution
@05:26:11.11 delay 2 seconds
@05:26:13.14 AS.reconfigure ASconfig
@05:26:13.19 AS.seek 0.5ml
@05:26:42.23 delay 1 second
@05:26:43.25 AFV.select :holdingCoil
@05:26:43.77 ATV.dial :extSrc
@05:26:43.79 AS.reconfigure ASslow1
@05:26:43.86 AS.seek 0ml
@05:28:24.72 AFV.select :carrierSolution
@05:28:24.83 delay 2 seconds
@05:28:26.86 AS.reconfigure ASconfig
@05:28:26.91 AS.seek 0.5ml
@05:28:55.95 delay 1 second
@05:28:56.97 AFV.select :holdingCoil
@05:28:57.07 ATV.dial :extSrc
@05:28:57.09 AS.reconfigure ASslow1
@05:28:57.16 AS.seek 0ml
@05:30:38.00 AFV.select :carrierSolution
@05:30:38.11 delay 2 seconds
@05:30:40.14 AS.reconfigure ASconfig
@05:30:40.19 AS.seek 0.5ml
@05:31:09.25 delay 1 second
@05:31:10.27 AFV.select :holdingCoil
@05:31:10.37 ATV.dial :extSrc
@05:31:10.39 AS.reconfigure ASslow1
@05:31:10.46 AS.seek 0ml
@05:32:51.47 AFV.select :carrierSolution
@05:32:51.58 delay 2 seconds
@05:32:53.61 AS.reconfigure ASconfig
@05:32:53.66 AS.seek 0.5ml
@05:33:22.67 delay 1 second
@05:33:23.69 AFV.select :holdingCoil
@05:33:23.79 ATV.dial :extSrc
@05:33:23.81 AS.reconfigure ASslow1
@05:33:23.88 AS.seek 0ml
@05:35:04.73 ATV.dial :air2
@05:35:06.12 AS.reconfigure ASslow0
@05:35:06.18 AS.seek 0.5ml
@05:35:56.72 ATV.dial :extSrc
@05:35:58.29 AS.seek 0.02ml
@05:36:46.83 delay 1 second
@05:36:47.85 ATV.dial :waste
@05:36:49.22 AS.reconfigure ASconfig
@05:36:49.28 AS.seek 0ml
@05:36:50.75 AFV.select :holdingCoil
@05:36:50.76 ATV.dial :waste
@05:36:50.79 AS.seek 0ml
@05:36:50.87 AFV.select :carrierSolution
@05:36:50.98 delay 2 seconds
@05:36:53.01 AS.seek 0.5ml
@05:37:22.11 delay 1 second
@05:37:23.13 AFV.select :holdingCoil
@05:37:23.23 ATV.dial :air3
@05:37:24.09 AS.reconfigure ASslow1
@05:37:24.15 AS.seek 0.52ml
@05:37:28.47 ATV.dial :air3
@05:37:28.55 AS.seek 0.53ml
@05:37:30.88 delay 2 seconds
@05:37:32.90 ATV.dial :bleach
@05:37:33.40 AS.seek 0.71ml
@05:38:09.84 delay 3 seconds
@05:38:12.86 ATV.dial :air3
@05:38:13.45 delay 3 seconds
@05:38:16.52 AS.seek 0.735ml
@05:38:21.85 delay 3 seconds
@05:38:24.87 ATV.dial :bleach
@05:38:25.53 AS.seek 0.72ml
@05:38:28.84 delay 3 seconds
@05:38:31.86 ATV.dial ASV
@05:38:32.97 ASV.dial :SPErecovery
@05:38:33.05 AS.seek 0.365ml
@05:39:44.64 ATV.dial :waste
@05:39:46.45 AS.reconfigure ASnorm
@05:39:46.51 AS.seek 0ml
@05:40:05.26 ATV.dial :air3
@05:40:05.97 AS.reconfigure ASslow1
@05:40:06.03 AS.seek 0.01ml
@05:40:08.34 delay 2 seconds
@05:40:10.36 ATV.dial :bleach
@05:40:10.84 AS.seek 0.58ml
@05:42:05.73 delay 3 seconds
@05:42:08.75 ATV.dial :air3
@05:42:09.36 delay 3 seconds
@05:42:12.43 AS.seek 0.605ml
@05:42:17.73 delay 3 seconds
@05:42:20.75 ATV.dial :bleach
@05:42:21.40 AS.seek 0.59ml
@05:42:24.81 delay 3 seconds
@05:42:27.83 ATV.dial :ASV
@05:42:28.94 ASV.dial :wasteR
@05:42:29.34 AS.seek 0ml
@05:44:28.29 delay 1:00
@05:45:28.32 ATV.dial ASV
@05:45:28.33 AFV.select :carrierSolution
@05:45:28.45 delay 2 seconds
@05:45:30.47 AS.reconfigure ASconfig
@05:45:30.53 AS.seek 1ml
@05:46:28.29 delay 1 second
@05:46:29.31 AFV.select :holdingCoil
@05:46:29.41 delay 1 second
@05:46:30.43 ASV.dial :wasteR
@05:46:30.45 AS.reconfigure ASslow1
@05:46:30.52 AS.seek 0ml
@05:49:51.93 delay 10 seconds
@05:50:01.96 ATV.dial :air3
@05:50:03.57 AS.seek 0.01ml
@05:50:05.87 delay 2 seconds
@05:50:07.89 ATV.dial :bleach
@05:50:08.56 AS.seek 0.58ml
@05:52:03.45 delay 3 seconds
@05:52:06.47 ATV.dial :air3
@05:52:07.06 delay 3 seconds
@05:52:10.13 AS.seek 0.605ml
@05:52:15.54 delay 3 seconds
@05:52:18.93 ATV.dial :bleach
@05:52:19.64 AS.seek 0.59ml
@05:52:22.97 delay 3 seconds
@05:52:25.99 ATV.dial ASV
@05:52:27.09 ASV.dial :SPEdelivery
@05:52:27.48 AS.seek 0ml
@05:54:26.42 delay 1:00
@05:55:26.45 AFV.select :carrierSolution
@05:55:26.55 delay 2 seconds
@05:55:28.58 AS.reconfigure ASconfig
@05:55:28.63 AS.seek 1ml
@05:56:26.39 delay 1 second
@05:56:27.41 AFV.select :holdingCoil
@05:56:27.51 delay 1 second
@05:56:28.53 ATV.dial ASV
@05:56:28.54 ASV.dial :SPEdelivery
@05:56:28.57 AS.reconfigure ASslow1
@05:56:28.63 AS.seek 0ml
@05:59:50.01 delay 10 seconds
@06:00:00.04 ATV.dial :air2
@06:00:00.92 AS.reconfigure ASslow0
@06:00:00.98 AS.seek 0.8ml
@06:01:21.79 delay 3 seconds
@06:01:24.81 ATV.dial :ASV
@06:01:25.86 ASV.dial :SPEdelivery
@06:01:25.88 AS.reconfigure ASslow1
@06:01:25.95 AS.seek 0.03ml
@06:04:01.20 delay 20 seconds
@06:04:21.23 ATV.dial :waste,via: :air3
@06:04:23.08 AS.reconfigure ASconfig
@06:04:23.14 AS.seek 0ml
@06:04:25.18 SPE.coast
@06:04:25.26 Removing Bleach from Mixing Coil
@06:04:25.27 AFV.select :carrierSolution
@06:04:25.39 delay 2 seconds
@06:04:27.42 AS.seek 0.5ml
@06:04:56.47 delay 1 second
@06:04:57.49 AFV.select :holdingCoil
@06:04:57.59 ATV.dial :ASV
@06:04:59.39 ASV.dial :SPErecovery
@06:05:00.03 AS.reconfigure ASslow1
@06:05:00.09 AS.seek 0.73ml
@06:05:46.64 ASV.dial :wasteD
@06:05:47.25 AS.reconfigure ASnorm
@06:05:47.31 AS.seek 0ml
@06:06:24.43 AFV.select :carrierSolution
@06:06:24.54 delay 2 seconds
@06:06:26.57 AS.reconfigure ASconfig
@06:06:26.62 AS.seek 0.5ml
@06:06:55.67 delay 1 second
@06:06:56.69 AFV.select :holdingCoil
@06:06:56.79 ATV.dial ASV
@06:06:56.81 ASV.dial :SPErecovery
@06:06:57.48 AS.reconfigure ASslow0
@06:06:57.54 AS.seek 0.3ml
@06:07:17.93 ATV.dial :air2
@06:07:19.03 AS.reconfigure ASslow1
@06:07:19.09 AS.seek 0.32ml
@06:07:23.54 ATV.dial ASV
@06:07:24.63 AS.reconfigure ASslow0
@06:07:24.69 AS.seek 0.57ml
@06:07:50.15 ASV.dial :wasteD
@06:07:50.75 AS.reconfigure ASnorm
@06:07:50.81 AS.seek 0ml
@06:08:19.89 AFV.select :carrierSolution
@06:08:20.00 delay 2 seconds
@06:08:22.03 AS.reconfigure ASconfig
@06:08:22.08 AS.seek 0.5ml
@06:08:51.11 delay 1 second
@06:08:52.13 AFV.select :holdingCoil
@06:08:52.23 ATV.dial ASV
@06:08:52.25 ASV.dial :SPErecovery
@06:08:52.91 AS.reconfigure ASslow0
@06:08:52.97 AS.seek 0.3ml
@06:09:13.42 ATV.dial :air2
@06:09:14.49 AS.reconfigure ASslow1
@06:09:14.55 AS.seek 0.32ml
@06:09:18.86 ATV.dial ASV
@06:09:19.94 AS.reconfigure ASslow0
@06:09:20.00 AS.seek 0.57ml
@06:09:45.50 ASV.dial :wasteD
@06:09:46.11 AS.reconfigure ASnorm
@06:09:46.17 AS.seek 0ml
@06:10:15.22 ATV.dial :air2
@06:10:16.25 AS.reconfigure ASslow0
@06:10:16.31 AS.seek 0.3ml
@06:10:46.89 ATV.dial :ASV
@06:10:47.93 ASV.dial :wasteD
@06:10:47.95 AS.reconfigure ASconfig
@06:10:48.01 AS.seek 0.05ml
@06:11:02.68 ATV.dial :waste
@06:11:04.65 AS.seek 0ml
@06:11:07.80 ASV.dial :SPEdelivery
@06:11:08.89 Cleaning PCR
@06:11:08.90 AFV.select :carrierSolution
@06:11:09.01 delay 2 seconds
@06:11:11.04 AS.seek 0.5ml
@06:11:40.09 delay 1 second
@06:11:41.11 AFV.select :holdingCoil
@06:11:41.21 ASV.dial :waste1
@06:11:41.59 ATV.dial :air3
@06:11:42.30 AS.reconfigure ASslow1
@06:11:42.37 AS.seek 0.51ml
@06:11:44.76 delay 2 seconds
@06:11:46.78 ATV.dial :bleach
@06:11:47.26 AS.seek 0.81ml
@06:12:47.86 delay 3 seconds
@06:12:51.31 ATV.dial :air3
@06:12:51.90 delay 3 seconds
@06:12:54.97 AS.seek 0.835ml
@06:13:00.27 delay 3 seconds
@06:13:03.29 ATV.dial :bleach
@06:13:03.95 AS.seek 0.82ml
@06:13:07.26 delay 3 seconds
@06:13:10.28 ARV.dial :waste
@06:13:10.92 delay 3 seconds
@06:13:13.94 ATV.dial :ARV
@06:13:14.86 AS.reconfigure ASconfig
@06:13:14.93 AS.seek 0.47ml
@06:13:35.36 AFV.select :carrierSolution
@06:13:35.47 delay 2 seconds
@06:13:37.50 AS.seek 1ml
@06:14:08.28 delay 1 second
@06:14:09.30 AFV.select :holdingCoil
@06:14:09.40 delay 1 second
@06:14:10.43 AS.reconfigure ASfast
@06:14:10.48 AS.seek 0ml
@06:14:37.83 AFV.select :carrierSolution
@06:14:37.94 delay 2 seconds
@06:14:39.97 AS.reconfigure ASconfig
@06:14:40.02 AS.seek 1ml
@06:15:37.81 delay 1 second
@06:15:38.83 AFV.select :holdingCoil
@06:15:38.93 delay 1 second
@06:15:39.96 AS.reconfigure ASfast
@06:15:40.01 AS.seek 0ml
@06:16:07.37 AFV.select :carrierSolution
@06:16:07.48 delay 2 seconds
@06:16:09.51 AS.reconfigure ASconfig
@06:16:09.56 AS.seek 1ml
@06:17:07.36 delay 1 second
@06:17:08.38 AFV.select :holdingCoil
@06:17:08.48 delay 1 second
@06:17:09.51 AS.reconfigure ASfast
@06:17:09.56 AS.seek 0ml
@06:17:36.89 ARV.dial between carrier and air1
@06:17:37.42 ATV.dial :air3
@06:17:38.78 AS.reconfigure ASslow1
@06:17:38.84 AS.seek 0.01ml
@06:17:41.23 delay 2 seconds
@06:17:43.25 ATV.dial :bleach
@06:17:43.90 AS.seek 0.81ml
@06:20:25.06 delay 3 seconds
@06:20:28.08 ATV.dial :air3
@06:20:28.67 delay 3 seconds
@06:20:31.74 AS.seek 0.835ml
@06:20:37.05 delay 3 seconds
@06:20:40.07 ATV.dial :bleach
@06:20:40.71 AS.seek 0.82ml
@06:20:44.12 delay 3 seconds
@06:20:47.14 ATV.dial PCR
@06:20:47.56 AS.reconfigure ASconfig
@06:20:47.62 AS.seek 0ml
@06:21:35.04 PCR.program :bleachProfile
@06:21:35.10 PCR program should complete in < 2:45
@06:21:35.11 PCR.run 10:00
@06:24:37.38 PCR.off
@06:24:37.42 AFV.select :carrierSolution
@06:24:37.53 delay 2 seconds
@06:24:39.56 AS.seek 1ml
@06:25:37.34 delay 1 second
@06:25:38.36 AFV.select :holdingCoil
@06:25:38.46 delay 1 second
@06:25:39.49 AS.reconfigure ASfast
@06:25:39.54 AS.seek 0ml
@06:26:06.89 delay 3 seconds
@06:26:09.91 AFV.select :carrierSolution
@06:26:10.01 delay 2 seconds
@06:26:12.04 AS.reconfigure ASconfig
@06:26:12.25 AS.seek 1ml
@06:27:10.03 delay 1 second
@06:27:11.05 AFV.select :holdingCoil
@06:27:11.15 delay 1 second
@06:27:12.18 AS.reconfigure ASfast
@06:27:12.23 AS.seek 0ml
@06:27:39.58 delay 3 seconds
@06:27:42.60 AFV.select :carrierSolution
@06:27:42.70 delay 2 seconds
@06:27:44.73 AS.reconfigure ASconfig
@06:27:44.79 AS.seek 1ml
@06:28:42.58 delay 1 second
@06:28:43.60 AFV.select :holdingCoil
@06:28:43.70 delay 1 second
@06:28:44.73 AS.reconfigure ASfast
@06:28:44.78 AS.seek 0ml
@06:29:12.12 delay 3 seconds
@06:29:15.14 AFV.select :carrierSolution
@06:29:15.24 delay 2 seconds
@06:29:17.27 AS.reconfigure ASconfig
@06:29:17.33 AS.seek 1ml
@06:30:15.08 delay 1 second
@06:30:16.10 AFV.select :holdingCoil
@06:30:16.20 delay 1 second
@06:30:17.23 AS.reconfigure ASfast
@06:30:17.28 AS.seek 0ml
@06:30:44.62 delay 3 seconds
@06:30:47.64 AFV.select :carrierSolution
@06:30:47.74 delay 2 seconds
@06:30:49.77 AS.reconfigure ASconfig
@06:30:49.83 AS.seek 1ml
@06:31:47.58 delay 1 second
@06:31:48.60 AFV.select :holdingCoil
@06:31:48.70 delay 1 second
@06:31:49.73 AS.reconfigure ASnorm
@06:31:49.78 AS.seek 0ml
@06:32:40.52 AFV.select :carrierSolution
@06:32:40.62 delay 2 seconds
@06:32:42.64 AS.reconfigure ASconfig
@06:32:42.70 AS.seek 1ml
@06:33:40.48 delay 1 second
@06:33:41.50 AFV.select :holdingCoil
@06:33:41.60 delay 1 second
@06:33:42.62 ATV.dial :waste
@06:33:43.90 AS.reconfigure ASnorm
@06:33:43.96 AS.seek 0.5ml
@06:34:09.53 ATV.dial :waste2
@06:34:10.87 AS.seek 0ml
@06:34:36.45 <MAIN> Cleaning PCR
@06:34:36.46 AFV.select :carrierSolution
@06:34:36.58 delay 2 seconds
@06:34:38.61 AS.reconfigure ASconfig
@06:34:38.66 AS.seek 0.5ml
@06:35:07.70 delay 1 second
@06:35:08.72 AFV.select :holdingCoil
@06:35:08.82 ASV.dial :waste1
@06:35:08.83 ATV.dial :air3
@06:35:10.88 AS.reconfigure ASslow1
@06:35:10.94 AS.seek 0.51ml
@06:35:13.24 delay 2 seconds
@06:35:15.26 ATV.dial :bleach
@06:35:15.76 AS.seek 0.81ml
@06:36:16.47 delay 3 seconds
@06:36:19.49 ATV.dial :air3
@06:36:20.09 delay 3 seconds
@06:36:23.16 AS.seek 0.835ml
@06:36:28.48 delay 3 seconds
@06:36:31.50 ATV.dial :bleach
@06:36:32.15 AS.seek 0.82ml
@06:36:35.47 delay 3 seconds
@06:36:38.49 ARV.dial :waste
@06:36:39.01 delay 3 seconds
@06:36:42.03 ATV.dial :ARV
@06:36:42.95 AS.reconfigure ASconfig
@06:36:43.01 AS.seek 0.47ml
@06:37:03.42 AFV.select :carrierSolution
@06:37:03.53 delay 2 seconds
@06:37:05.56 AS.seek 1ml
@06:37:36.29 delay 1 second
@06:37:37.31 AFV.select :holdingCoil
@06:37:37.41 delay 1 second
@06:37:38.44 AS.reconfigure ASfast
@06:37:38.49 AS.seek 0ml
@06:38:05.83 AFV.select :carrierSolution
@06:38:05.94 delay 2 seconds
@06:38:07.96 AS.reconfigure ASconfig
@06:38:08.02 AS.seek 1ml
@06:39:05.81 delay 1 second
@06:39:06.83 AFV.select :holdingCoil
@06:39:06.93 delay 1 second
@06:39:07.96 AS.reconfigure ASfast
@06:39:08.01 AS.seek 0ml
@06:39:35.36 AFV.select :carrierSolution
@06:39:35.48 delay 2 seconds
@06:39:37.50 AS.reconfigure ASconfig
@06:39:37.61 AS.seek 1ml
@06:40:35.42 delay 1 second
@06:40:36.44 AFV.select :holdingCoil
@06:40:36.54 delay 1 second
@06:40:37.57 AS.reconfigure ASfast
@06:40:37.62 AS.seek 0ml
@06:41:04.95 ARV.dial between carrier and air1
@06:41:05.48 ATV.dial :air3
@06:41:06.84 AS.reconfigure ASslow1
@06:41:06.90 AS.seek 0.01ml
@06:41:09.37 delay 2 seconds
@06:41:11.39 ATV.dial :bleach
@06:41:12.06 AS.seek 0.81ml
@06:43:53.23 delay 3 seconds
@06:43:56.25 ATV.dial :air3
@06:43:56.86 delay 3 seconds
@06:43:59.93 AS.seek 0.835ml
@06:44:05.23 delay 3 seconds
@06:44:08.25 ATV.dial :bleach
@06:44:08.90 AS.seek 0.82ml
@06:44:12.61 delay 3 seconds
@06:44:15.63 ATV.dial PCR
@06:44:16.04 AS.reconfigure ASconfig
@06:44:16.10 AS.seek 0ml
@06:45:03.51 PCR.program :bleachProfile
@06:45:03.57 PCR program should complete in < 2:45
@06:45:03.58 PCR.run 10:00
@06:48:05.59 PCR.off
@06:48:05.63 AFV.select :carrierSolution
@06:48:05.74 delay 2 seconds
@06:48:07.77 AS.seek 1ml
@06:49:05.56 delay 1 second
@06:49:06.58 AFV.select :holdingCoil
@06:49:06.68 delay 1 second
@06:49:07.71 AS.reconfigure ASfast
@06:49:07.76 AS.seek 0ml
@06:49:35.11 delay 3 seconds
@06:49:38.13 AFV.select :carrierSolution
@06:49:38.23 delay 2 seconds
@06:49:40.25 AS.reconfigure ASconfig
@06:49:40.31 AS.seek 1ml
@06:50:38.04 delay 1 second
@06:50:39.06 AFV.select :holdingCoil
@06:50:39.16 delay 1 second
@06:50:40.19 AS.reconfigure ASfast
@06:50:40.24 AS.seek 0ml
@06:51:07.58 delay 3 seconds
@06:51:10.60 AFV.select :carrierSolution
@06:51:10.70 delay 2 seconds
@06:51:12.73 AS.reconfigure ASconfig
@06:51:12.79 AS.seek 1ml
@06:52:10.57 delay 1 second
@06:52:11.59 AFV.select :holdingCoil
@06:52:11.69 delay 1 second
@06:52:12.72 AS.reconfigure ASfast
@06:52:12.77 AS.seek 0ml
@06:52:40.11 delay 3 seconds
@06:52:43.13 AFV.select :carrierSolution
@06:52:43.23 delay 2 seconds
@06:52:45.25 AS.reconfigure ASconfig
@06:52:45.32 AS.seek 1ml
@06:53:43.12 delay 1 second
@06:53:44.14 AFV.select :holdingCoil
@06:53:44.24 delay 1 second
@06:53:45.27 AS.reconfigure ASfast
@06:53:45.32 AS.seek 0ml
@06:54:12.67 delay 3 seconds
@06:54:15.69 AFV.select :carrierSolution
@06:54:15.79 delay 2 seconds
@06:54:17.81 AS.reconfigure ASconfig
@06:54:17.89 AS.seek 1ml
@06:55:15.67 delay 1 second
@06:55:16.69 AFV.select :holdingCoil
@06:55:16.79 delay 1 second
@06:55:17.81 AS.reconfigure ASnorm
@06:55:17.87 AS.seek 0ml
@06:56:08.59 delay 5 seconds
@06:56:13.66 Cleaning PCR
@06:56:13.67 AFV.select :carrierSolution
@06:56:13.79 delay 2 seconds
@06:56:15.82 AS.reconfigure ASconfig
@06:56:15.87 AS.seek 0.5ml
@06:56:44.90 delay 1 second
@06:56:45.92 AFV.select :holdingCoil
@06:56:46.02 ASV.dial :waste1
@06:56:46.03 ATV.dial :air3
@06:56:46.92 AS.reconfigure ASslow1
@06:56:46.98 AS.seek 0.51ml
@06:56:49.34 delay 2 seconds
@06:56:51.36 ATV.dial :bleach
@06:56:52.01 AS.seek 0.81ml
@06:57:52.95 delay 3 seconds
@06:57:55.97 ATV.dial :air3
@06:57:56.57 delay 3 seconds
@06:57:59.64 AS.seek 0.835ml
@06:58:04.96 delay 3 seconds
@06:58:07.98 ATV.dial :bleach
@06:58:08.63 AS.seek 0.82ml
@06:58:12.04 delay 3 seconds
@06:58:15.06 ARV.dial :waste
@06:58:15.57 delay 3 seconds
@06:58:18.59 ATV.dial :ARV
@06:58:19.52 AS.reconfigure ASconfig
@06:58:19.58 AS.seek 0.47ml
@06:58:40.00 AFV.select :carrierSolution
@06:58:40.11 delay 2 seconds
@06:58:42.14 AS.seek 1ml
@06:59:12.87 delay 1 second
@06:59:13.89 AFV.select :holdingCoil
@06:59:13.99 delay 1 second
@06:59:15.02 AS.reconfigure ASfast
@06:59:15.07 AS.seek 0ml
@06:59:42.40 AFV.select :carrierSolution
@06:59:42.51 delay 2 seconds
@06:59:44.54 AS.reconfigure ASconfig
@06:59:44.59 AS.seek 1ml
@07:00:42.37 delay 1 second
@07:00:43.39 AFV.select :holdingCoil
@07:00:43.49 delay 1 second
@07:00:44.52 AS.reconfigure ASfast
@07:00:44.57 AS.seek 0ml
@07:01:11.90 AFV.select :carrierSolution
@07:01:12.01 delay 2 seconds
@07:01:14.03 AS.reconfigure ASconfig
@07:01:14.09 AS.seek 1ml
@07:02:11.84 delay 1 second
@07:02:12.86 AFV.select :holdingCoil
@07:02:12.96 delay 1 second
@07:02:13.99 AS.reconfigure ASfast
@07:02:14.04 AS.seek 0ml
@07:02:41.37 ARV.dial between carrier and air1
@07:02:41.89 ATV.dial :air3
@07:02:43.23 AS.reconfigure ASslow1
@07:02:43.29 AS.seek 0.01ml
@07:02:45.59 delay 2 seconds
@07:02:47.61 ATV.dial :bleach
@07:02:48.26 AS.seek 0.81ml
@07:05:29.50 delay 3 seconds
@07:05:32.52 ATV.dial :air3
@07:05:33.12 delay 3 seconds
@07:05:36.19 AS.seek 0.835ml
@07:05:41.51 delay 3 seconds
@07:05:44.53 ATV.dial :bleach
@07:05:45.18 AS.seek 0.82ml
@07:05:48.47 delay 3 seconds
@07:05:51.49 ATV.dial PCR
@07:05:51.92 AS.reconfigure ASconfig
@07:05:51.98 AS.seek 0ml
@07:06:39.39 PCR.program :bleachProfile
@07:06:39.45 PCR program should complete in < 2:45
@07:06:39.46 PCR.run 10:00
@07:09:38.40 PCR.off
@07:09:38.44 AFV.select :carrierSolution
@07:09:38.85 delay 2 seconds
@07:09:40.94 AS.seek 1ml
@07:10:38.73 delay 1 second
@07:10:39.75 AFV.select :holdingCoil
@07:10:39.85 delay 1 second
@07:10:40.88 AS.reconfigure ASfast
@07:10:40.93 AS.seek 0ml
@07:11:08.26 delay 3 seconds
@07:11:11.28 AFV.select :carrierSolution
@07:11:11.38 delay 2 seconds
@07:11:13.41 AS.reconfigure ASconfig
@07:11:13.47 AS.seek 1ml
@07:12:11.22 delay 1 second
@07:12:12.24 AFV.select :holdingCoil
@07:12:12.34 delay 1 second
@07:12:13.37 AS.reconfigure ASfast
@07:12:13.42 AS.seek 0ml
@07:12:40.76 delay 3 seconds
@07:12:43.78 AFV.select :carrierSolution
@07:12:43.88 delay 2 seconds
@07:12:45.91 AS.reconfigure ASconfig
@07:12:45.97 AS.seek 1ml
@07:13:43.72 delay 1 second
@07:13:44.74 AFV.select :holdingCoil
@07:13:44.84 delay 1 second
@07:13:45.87 AS.reconfigure ASfast
@07:13:45.92 AS.seek 0ml
@07:14:13.26 delay 3 seconds
@07:14:16.28 AFV.select :carrierSolution
@07:14:16.38 delay 2 seconds
@07:14:18.40 AS.reconfigure ASconfig
@07:14:18.47 AS.seek 1ml
@07:15:16.22 delay 1 second
@07:15:17.24 AFV.select :holdingCoil
@07:15:17.34 delay 1 second
@07:15:18.37 AS.reconfigure ASfast
@07:15:18.42 AS.seek 0ml
@07:15:45.76 delay 3 seconds
@07:15:48.78 AFV.select :carrierSolution
@07:15:48.88 delay 2 seconds
@07:15:50.90 AS.reconfigure ASconfig
@07:15:50.97 AS.seek 1ml
@07:16:48.70 delay 1 second
@07:16:49.72 AFV.select :holdingCoil
@07:16:49.82 delay 1 second
@07:16:50.85 AS.reconfigure ASnorm
@07:16:50.90 AS.seek 0ml
@07:17:41.66 delay 5 seconds
@07:17:46.73 Cleaning PCR
@07:17:46.74 AFV.select :carrierSolution
@07:17:46.86 delay 2 seconds
@07:17:48.89 AS.reconfigure ASconfig
@07:17:48.94 AS.seek 0.5ml
@07:18:17.98 delay 1 second
@07:18:19.00 AFV.select :holdingCoil
@07:18:19.10 ASV.dial :waste1
@07:18:19.11 ATV.dial :air3
@07:18:20.02 AS.reconfigure ASslow1
@07:18:20.08 AS.seek 0.51ml
@07:18:22.39 delay 2 seconds
@07:18:24.41 ATV.dial :bleach
@07:18:25.06 AS.seek 0.81ml
@07:19:25.70 delay 3 seconds
@07:19:28.72 ATV.dial :air3
@07:19:29.31 delay 3 seconds
@07:19:32.38 AS.seek 0.835ml
@07:19:37.70 delay 3 seconds
@07:19:40.72 ATV.dial :bleach
@07:19:41.37 AS.seek 0.82ml
@07:19:44.65 delay 3 seconds
@07:19:47.67 ARV.dial :waste
@07:19:48.17 delay 3 seconds
@07:19:51.19 ATV.dial :ARV
@07:19:52.14 AS.reconfigure ASconfig
@07:19:52.20 AS.seek 0.47ml
@07:20:12.59 AFV.select :carrierSolution
@07:20:12.70 delay 2 seconds
@07:20:14.73 AS.seek 1ml
@07:20:45.48 delay 1 second
@07:20:46.50 AFV.select :holdingCoil
@07:20:46.60 delay 1 second
@07:20:47.63 AS.reconfigure ASfast
@07:20:47.68 AS.seek 0ml
@07:21:15.00 AFV.select :carrierSolution
@07:21:15.11 delay 2 seconds
@07:21:17.13 AS.reconfigure ASconfig
@07:21:17.19 AS.seek 1ml
@07:22:14.93 delay 1 second
@07:22:15.95 AFV.select :holdingCoil
@07:22:16.05 delay 1 second
@07:22:17.08 AS.reconfigure ASfast
@07:22:17.13 AS.seek 0ml
@07:22:44.46 AFV.select :carrierSolution
@07:22:44.57 delay 2 seconds
@07:22:46.59 AS.reconfigure ASconfig
@07:22:46.65 AS.seek 1ml
@07:23:44.39 delay 1 second
@07:23:45.41 AFV.select :holdingCoil
@07:23:45.51 delay 1 second
@07:23:46.54 AS.reconfigure ASfast
@07:23:46.59 AS.seek 0ml
@07:24:13.92 ARV.dial between carrier and air1
@07:24:14.45 ATV.dial :air3
@07:24:15.81 AS.reconfigure ASslow1
@07:24:15.87 AS.seek 0.01ml
@07:24:18.22 delay 2 seconds
@07:24:20.24 ATV.dial :bleach
@07:24:20.88 AS.seek 0.81ml
@07:27:02.12 delay 3 seconds
@07:27:05.14 ATV.dial :air3
@07:27:05.73 delay 3 seconds
@07:27:08.80 AS.seek 0.835ml
@07:27:14.22 delay 3 seconds
@07:27:17.24 ATV.dial :bleach
@07:27:17.88 AS.seek 0.82ml
@07:27:21.17 delay 3 seconds
@07:27:24.19 ATV.dial PCR
@07:27:24.61 AS.reconfigure ASconfig
@07:27:24.67 AS.seek 0ml
@07:28:12.07 PCR.program :bleachProfile
@07:28:12.13 PCR program should complete in < 2:45
@07:28:12.14 PCR.run 10:00
@07:31:11.34 PCR.off
@07:31:11.39 AFV.select :carrierSolution
@07:31:11.50 delay 2 seconds
@07:31:13.53 AS.seek 1ml
@07:32:11.28 delay 1 second
@07:32:12.30 AFV.select :holdingCoil
@07:32:12.40 delay 1 second
@07:32:13.43 AS.reconfigure ASfast
@07:32:13.48 AS.seek 0ml
@07:32:40.81 delay 3 seconds
@07:32:43.83 AFV.select :carrierSolution
@07:32:43.93 delay 2 seconds
@07:32:45.95 AS.reconfigure ASconfig
@07:32:46.02 AS.seek 1ml
@07:33:43.81 delay 1 second
@07:33:44.83 AFV.select :holdingCoil
@07:33:44.93 delay 1 second
@07:33:45.96 AS.reconfigure ASfast
@07:33:46.01 AS.seek 0ml
@07:34:13.36 delay 3 seconds
@07:34:16.38 AFV.select :carrierSolution
@07:34:16.48 delay 2 seconds
@07:34:18.50 AS.reconfigure ASconfig
@07:34:18.56 AS.seek 1ml
@07:35:16.32 delay 1 second
@07:35:17.34 AFV.select :holdingCoil
@07:35:17.44 delay 1 second
@07:35:18.47 AS.reconfigure ASfast
@07:35:18.52 AS.seek 0ml
@07:35:45.86 delay 3 seconds
@07:35:48.88 AFV.select :carrierSolution
@07:35:48.98 delay 2 seconds
@07:35:51.00 AS.reconfigure ASconfig
@07:35:51.07 AS.seek 1ml
@07:36:48.87 delay 1 second
@07:36:49.89 AFV.select :holdingCoil
@07:36:49.99 delay 1 second
@07:36:51.02 AS.reconfigure ASfast
@07:36:51.07 AS.seek 0ml
@07:37:18.42 delay 3 seconds
@07:37:21.44 AFV.select :carrierSolution
@07:37:21.54 delay 2 seconds
@07:37:23.56 AS.reconfigure ASconfig
@07:37:23.62 AS.seek 1ml
@07:38:21.36 delay 1 second
@07:38:22.38 AFV.select :holdingCoil
@07:38:22.48 delay 1 second
@07:38:23.51 AS.reconfigure ASnorm
@07:38:23.56 AS.seek 0ml
@07:39:14.31 delay 5 seconds
@07:39:19.39 <bkha> Session Ended
@12:02:03.34CEST22-Apr-21 <MAIN> ESP2 v3.30 I2C Gateway v3.06 for core bus
@12:02:05.42 
Configuration for mer 7/20/20 brent@mbari.org
/home/esp/esp2/type/shallow/mer/configure.rb
@12:02:05.51 
Configuration for MFB3 by preston@mbari.org
/home/esp/esp2/type/mfb/MFB3/configure.rb
@12:02:10.70 Polling Can every 10:00
@12:02:12.54 CS.configure :default
@12:02:12.58 CS.hold
@12:02:12.65 CS.configure :default
@12:02:12.73 PS.configure :default
@12:02:12.81 SS.configure :default
@12:02:12.85 SS.hold
@12:02:12.91 SS.configure :default
@12:02:12.99 CC.configure :default
@12:02:13.03 CC.hold
@12:02:13.09 CC.configure :default
@12:02:13.18 PC.configure :default
@12:02:13.22 PC.hold
@12:02:13.29 PC.configure :default
@12:02:13.39 Forearm.configure :loose
@12:02:13.42 Forearm.hold
@12:02:13.48 Forearm.configure :loose
@12:02:13.58 Elbow.configure :loose
@12:02:13.64 Elbow.hold
@12:02:13.74 Elbow.configure :loose
@12:02:13.94 SC.configure :loose
@12:02:14.02 SC.hold
@12:02:14.12 SC.configure :loose
@12:02:14.28 SE.configure :default
@12:02:14.34 SE.hold
@12:02:14.40 SE.configure :default
@12:02:14.44 CH.coast
@12:02:14.48 CH.configure :default
@12:02:14.51 PH.coast
@12:02:14.56 PH.configure :default
@12:02:14.65 SPE.coast
@12:02:14.70 SPE.configure SPEconfig
@12:02:14.77 AS.configure ASconfig
@12:02:14.81 AS.hold
@12:02:14.86 AS.configure ASconfig
@12:02:14.90 PCR.off
@12:02:14.93 PCR.pidtemp= 50,0.1,0,300,25,25
@12:02:14.96 PCR.caltemp= 2.5051,0.0372,0
@12:02:15.02 All dwarf controllers running firmware version 3.75
@12:02:15.40 Cleanly exited at 12:01:22CEST22-Apr-21
@12:02:15.46 Loading /home/esp/esp2/mission/GENOMAP_v3.rb mission
@12:02:15.80 <MFBPCR> Extracting Nucleic Acid from extSrc
@12:02:15.90 SPE.hold 2:46:40,55C
@12:02:15.94 <bkha> Session Begun
@12:02:15.98 <MFBPCR> ASV.dial :SPEdelivery
@12:02:16.50 delay 20 seconds
@12:02:36.53 AFV.select :holdingCoil
@12:02:36.63 ATV.dial :air2
@12:02:38.11 AS.reconfigure ASslow1
@12:02:38.17 AS.seek 0.07ml
@12:02:52.52 delay 3 seconds
@12:02:55.54 ATV.dial :extSrc
@12:02:57.05 AS.seek 0.47ml
@12:04:17.82 delay 5 seconds
@12:04:22.89 Solid Phase Extraction Heater at 40.7C
@12:04:22.90 ATV.dial ASV
@12:04:23.80 ASV.dial :SPEdelivery
@12:04:23.88 AS.seek 0.042ml
@12:05:50.21 delay 10 seconds
@12:06:00.24 Rinsing external lysate line extSrc
@12:06:00.25 AFV.select :carrierSolution
@12:06:00.39 delay 2 seconds
@12:06:02.41 AS.reconfigure ASconfig
@12:06:02.47 AS.seek 1ml
@12:06:57.82 delay 1 second
@12:06:58.84 AFV.select :holdingCoil
@12:06:58.94 delay 1 second
@12:06:59.96 ATV.dial :extSrc
@12:07:00.80 AS.reconfigure ASnorm
@12:07:00.86 AS.seek 0ml
@12:07:51.53 ATV.dial :air2
@12:07:53.13 AS.reconfigure ASslow0
@12:07:53.19 AS.seek 0.05ml
@12:07:58.52 ATV.dial :extSrc
@12:08:00.11 AS.seek 0.01ml
@12:08:04.48 ATV.dial :waste
@12:08:06.16 AS.reconfigure ASconfig
@12:08:06.27 AS.seek 0ml
@12:08:07.14 ethanol wash
@12:08:07.15 ATV.dial :air2
@12:08:08.34 AS.reconfigure ASslow2
@12:08:08.39 AS.seek 0.01ml
@12:08:19.24 delay 3 seconds
@12:08:22.26 Pulling 0.4ml of Ethanol Wash
@12:08:22.27 ATV.dial :ethanol
@12:08:23.17 AS.reconfigure ASslow1
@12:08:23.22 AS.seek 0.41ml
@12:09:43.97 delay 3 seconds
@12:09:46.99 ATV.dial :air1
@12:09:47.42 delay 3 seconds
@12:09:50.49 AS.seek 0.435ml
@12:09:55.79 delay 3 seconds
@12:09:58.81 ATV.dial :ethanol
@12:09:59.44 AS.seek 0.42ml
@12:10:02.78 delay 3 seconds
@12:10:05.80 ATV.dial ASV
@12:10:07.21 AS.seek 0.093ml
@12:11:13.27 delay 3 seconds
@12:11:16.29 ATV.dial :waste2
@12:11:17.10 AS.reconfigure ASconfig
@12:11:17.16 AS.seek 0ml
@12:11:22.80 ATV.dial :air2
@12:11:23.22 AS.reconfigure ASslow1
@12:11:23.28 AS.seek 1ml
@12:14:44.80 delay 5 seconds
@12:14:49.82 ATV.dial ASV
@12:14:50.86 ASV.dial :SPEdelivery
@12:14:50.88 AS.seek 0.02ml
@12:18:08.32 delay 5 seconds
@12:18:13.34 ATV.dial :air2
@12:18:14.44 AS.seek 0.52ml
@12:19:55.30 delay 5 seconds
@12:20:00.32 ATV.dial ASV
@12:20:01.38 ASV.dial :wasteR
@12:20:01.77 AS.seek 0.3ml
@12:20:46.33 delay 20 seconds
@12:21:06.37 AS.seek 0.07ml
@12:21:52.91 delay 20 seconds
@12:22:12.93 ATV.dial :waste2
@12:22:13.74 AS.reconfigure ASconfig
@12:22:13.80 AS.seek 0ml
@12:22:18.13 Elution
@12:22:18.14 ATV.dial :air1
@12:22:19.32 AS.reconfigure ASslow1
@12:22:19.38 AS.seek 0.05ml
@12:22:29.81 delay 3 seconds
@12:22:32.83 ATV.dial :ElutionWater
@12:22:33.52 AS.seek 0.11ml
@12:22:45.89 delay 3 seconds
@12:22:48.91 ATV.dial ASV,via: :air2
@12:22:51.15 ASV.dial :SPEelution
@12:22:51.77 AFV.select :carrierSolution
@12:22:51.93 AS.reconfigure ASnorm
@12:22:51.98 AS.seek 0.76ml
@12:23:25.08 delay 3 seconds
@12:23:28.10 AFV.select :holdingCoil
@12:23:28.25 AS.reconfigure ASslow1
@12:23:28.30 AS.seek 0.475ml
@12:24:25.96 delay 5 seconds
@12:24:31.04 AS.reconfigure ASslow2
@12:24:31.10 AS.seek 0.44ml
@12:25:06.42 delay 1:00
@12:26:06.45 pushing eluate to holding coil
@12:26:06.52 AS.seek 0.28ml
@12:28:47.63 delay 30 seconds
@12:29:17.66 ATV.dial :waste,via: :air3
@12:29:19.49 AS.reconfigure ASconfig
@12:29:19.55 AS.seek 0ml
@12:29:35.94 Parking Bleach in LysateSource line and SPEcolumn
@12:29:35.95 ATV.dial :air3
@12:29:36.83 AS.reconfigure ASslow1
@12:29:36.89 AS.seek 0.03ml
@12:29:43.22 ATV.dial :ElutionWater
@12:29:43.86 AS.seek 0.06ml
@12:29:50.14 ATV.dial :air3
@12:29:50.77 AS.seek 0.07ml
@12:29:53.05 delay 2 seconds
@12:29:55.07 ATV.dial :bleach
@12:29:55.55 AS.seek 0.15ml
@12:30:11.83 delay 3 seconds
@12:30:14.85 ATV.dial :air3
@12:30:15.46 delay 3 seconds
@12:30:18.53 AS.seek 0.175ml
@12:30:23.87 delay 3 seconds
@12:30:26.89 ATV.dial :bleach
@12:30:27.55 AS.seek 0.16ml
@12:30:30.85 delay 3 seconds
@12:30:33.87 ATV.dial :extSrc
@12:30:34.52 AS.reconfigure ASconfig
@12:30:34.58 AS.seek 0.015ml
@12:30:43.22 ATV.dial :waste
@12:30:44.77 AS.seek 0ml
@12:30:45.96 ATV.dial :air3
@12:30:46.83 AS.reconfigure ASslow1
@12:30:46.89 AS.seek 0.01ml
@12:30:49.24 delay 2 seconds
@12:30:51.26 ATV.dial :bleach
@12:30:51.74 AS.seek 0.58ml
@12:32:46.66 delay 3 seconds
@12:32:49.68 ATV.dial :air3
@12:32:50.28 delay 3 seconds
@12:32:53.35 AS.seek 0.605ml
@12:32:58.75 delay 3 seconds
@12:33:01.77 ATV.dial :bleach
@12:33:02.41 AS.seek 0.59ml
@12:33:05.80 delay 3 seconds
@12:33:08.82 ATV.dial :ASV
@12:33:09.92 ASV.dial :wasteR
@12:33:10.53 AS.seek 0ml
@12:35:09.45 delay 1:00
@12:36:09.48 AFV.select :carrierSolution
@12:36:09.58 delay 2 seconds
@12:36:11.60 AS.reconfigure ASconfig
@12:36:11.66 AS.seek 1ml
@12:37:09.42 delay 1 second
@12:37:10.44 AFV.select :holdingCoil
@12:37:10.54 delay 1 second
@12:37:11.56 ATV.dial ASV
@12:37:11.57 ASV.dial :wasteR
@12:37:11.60 AS.reconfigure ASslow1
@12:37:11.66 AS.seek 0ml
@12:40:33.03 delay 20 seconds
@12:40:53.06 ATV.dial :air3
@12:40:54.67 AS.seek 0.59ml
@12:42:53.69 delay 3 seconds
@12:42:56.71 ATV.dial :ASV
@12:42:58.24 ASV.dial :wasteR
@12:42:58.32 AS.seek 0.09ml
@12:44:39.11 delay 30 seconds
@12:45:09.14 ATV.dial :extSrc
@12:45:09.97 delay 3 seconds
@12:45:12.99 ATV.dial :ElutionWater
@12:45:14.19 AS.seek 0.12ml
@12:45:20.55 ATV.dial :air3
@12:45:21.20 AS.seek 0.13ml
@12:45:23.47 delay 2 seconds
@12:45:25.49 ATV.dial :bleach
@12:45:25.99 AS.seek 0.23ml
@12:45:46.45 delay 3 seconds
@12:45:49.47 ATV.dial :air3
@12:45:50.07 delay 3 seconds
@12:45:53.14 AS.seek 0.255ml
@12:45:58.50 delay 3 seconds
@12:46:01.52 ATV.dial :bleach
@12:46:02.25 AS.seek 0.24ml
@12:46:05.66 delay 3 seconds
@12:46:08.68 ATV.dial :ASV
@12:46:09.78 ASV.dial :SPEdelivery
@12:46:10.17 AFV.select :carrierSolution
@12:46:10.28 delay 2 seconds
@12:46:12.31 AS.reconfigure ASconfig
@12:46:12.36 AS.seek 0.5ml
@12:46:27.56 delay 1 second
@12:46:28.58 AFV.select :holdingCoil
@12:46:28.73 AS.reconfigure ASslow1
@12:46:28.78 AS.seek 0.17ml
@12:47:35.43 SPE.hold 2:46:40,95C
@12:47:35.48 delay 3:00
@12:50:35.53 AFV.select :carrierSolution
@12:50:35.64 delay 2 seconds
@12:50:37.66 AS.reconfigure ASconfig
@12:50:37.72 AS.seek 1ml
@12:51:25.72 delay 1 second
@12:51:26.74 AFV.select :holdingCoil
@12:51:26.84 delay 1 second
@12:51:27.86 ASV.dial :wasteD
@12:51:28.97 AS.seek 0ml
@12:52:26.77 Priming Eluate
@12:52:26.78 AFV.select :carrierSolution
@12:52:26.90 delay 2 seconds
@12:52:28.93 AS.seek 0.5ml
@12:52:57.95 delay 1 second
@12:52:58.97 AFV.select :holdingCoil
@12:52:59.07 ATV.dial ASV
@12:52:59.09 ASV.dial :SPErecovery
@12:52:59.72 Swish #1 of 2
@12:52:59.80 AS.reconfigure ASslow0
@12:52:59.86 AS.seek 0.6ml
@12:53:10.34 AS.seek 0.5ml
@12:53:20.70 Swish #2 of 2
@12:53:20.78 AS.seek 0.6ml
@12:53:31.29 AS.seek 0.5ml
@12:53:41.64 delay 3 seconds
@12:53:44.71 AS.reconfigure ASslow1
@12:53:44.77 AS.seek 0.684ml
@12:54:22.13 delay 3 seconds
@12:54:25.15 Eluate primed
@12:54:25.16 ATV.dial :waste2
@12:54:25.83 AS.reconfigure ASnorm
@12:54:25.89 AS.seek 0ml
@12:55:00.69 SPE.coast
@12:55:00.77 ASV.dial :SPErecovery
@12:55:00.79 Setting up PCR for SYBRIPc -- writing /var/log/esp/SYBRIPc21apr2212h.pcr
@12:55:00.81 Building PCR Slug from ASV with MM1 mix, PP6 primer
@12:55:00.82 Priming with carrier
@12:55:00.85 ATV.dial ARV
@12:55:01.91 ARV.dial :carrier
@12:55:02.28 AS.seek 0.5ml
@12:55:27.86 AFV.select :carrierSolution
@12:55:27.98 delay 2 seconds
@12:55:30.01 AS.reconfigure ASconfig
@12:55:30.07 AS.seek 1ml
@12:55:59.09 delay 1 second
@12:56:00.11 AFV.select :holdingCoil
@12:56:00.21 delay 1 second
@12:56:01.23 ATV.dial :waste
@12:56:02.83 AS.reconfigure ASnorm
@12:56:02.89 AS.seek 0ml
@12:56:53.59 AFV.select :carrierSolution
@12:56:53.71 delay 2 seconds
@12:56:55.74 AS.reconfigure ASconfig
@12:56:55.79 AS.seek 0.5ml
@12:57:24.88 delay 1 second
@12:57:25.90 AFV.select :holdingCoil
@12:57:26.00 Building Slug
@12:57:26.02 ATV.dial :ARV
@12:57:27.77 delay 2 seconds
@12:57:29.79 ARV.dial :air1
@12:57:30.20 delay 2 seconds
@12:57:32.27 AS.reconfigure ASslow2
@12:57:32.33 AS.seek 0.506ml
@12:57:39.00 delay 2 seconds
@12:57:41.02 ARV.dial :MM1
@12:57:41.67 delay 2 seconds
@12:57:43.69 Adding MM1
@12:57:43.77 AS.seek 0.524ml
@12:58:02.00 delay 25 seconds
@12:58:27.02 ARV.dial :PP6
@12:58:28.38 Adding PP6
@12:58:28.39 delay 2 seconds
@12:58:30.47 AS.seek 0.53ml
@12:58:36.94 delay 10 seconds
@12:58:46.97 ARV.dial :air1
@12:58:47.84 adding ARV air
@12:58:47.85 delay 2 seconds
@12:58:49.93 AS.seek 0.536ml
@12:58:56.34 delay 5 seconds
@12:59:01.36 ARV.dial :carrier
@12:59:01.83 delay 7 seconds
@12:59:08.91 AS.seek 0.594ml
@13:00:07.69 delay 5 seconds
@13:00:12.71 ATV.dial :ASV
@13:00:13.14 delay 2 seconds
@13:00:15.21 AS.seek 0.6ml
@13:00:21.77 delay 4 seconds
@13:00:25.79 ATV.dial ARV
@13:00:26.38 delay 2 seconds
@13:00:28.40 ARV.dial :carrier
@13:00:28.41 delay 2 seconds
@13:00:30.49 AS.seek 0.65ml
@13:01:20.92 delay 4 seconds
@13:01:24.94 ATV.dial PCR
@13:01:25.59 ASV.dial :waste1
@13:01:26.40 ARV.dial :air1
@13:01:26.80 Positioning Slug by 0.324ml
@13:01:26.89 AS.seek 0.326ml
@13:06:53.33 delay 5 seconds
@13:06:58.35 ATV.dial ARV
@13:06:59.17 ASV.dial :PCRlock
@13:06:59.55 delay 5 seconds
@13:07:04.57 Running SYBRIPc Profile60CIQ1 thermocycling
@13:07:04.59 <PCR> PCR.program Profile60CIQ1
@13:07:04.70 Profile60CIQ1 should complete in < 1:56:15
@13:07:04.71 PCR.run 2:30:18.75
@14:47:17.00 PCR.off
@14:47:17.55 <MFBPCR> PCR for SYBRIPc completed -- closed /var/log/esp/SYBRIPc21apr2212h.pcr
@14:47:17.63 Cleaning PCR
@14:47:17.64 AFV.select :carrierSolution
@14:47:17.76 delay 2 seconds
@14:47:19.79 AS.reconfigure ASconfig
@14:47:19.84 AS.seek 0.5ml
@14:47:30.14 delay 1 second
@14:47:31.16 AFV.select :holdingCoil
@14:47:31.26 ASV.dial :waste1
@14:47:31.67 ATV.dial :air3
@14:47:33.03 AS.reconfigure ASslow1
@14:47:33.10 AS.seek 0.51ml
@14:47:35.38 delay 2 seconds
@14:47:37.40 ATV.dial :bleach
@14:47:38.04 AS.seek 0.81ml
@14:48:38.75 delay 3 seconds
@14:48:41.77 ATV.dial :air3
@14:48:42.36 delay 3 seconds
@14:48:45.81 AS.seek 0.835ml
@14:48:51.20 delay 3 seconds
@14:48:54.22 ATV.dial :bleach
@14:48:54.87 AS.seek 0.82ml
@14:48:58.31 delay 3 seconds
@14:49:01.33 ARV.dial :waste
@14:49:01.97 delay 3 seconds
@14:49:04.99 ATV.dial :ARV
@14:49:05.91 AS.reconfigure ASconfig
@14:49:05.97 AS.seek 0.47ml
@14:49:26.37 AFV.select :carrierSolution
@14:49:26.48 delay 2 seconds
@14:49:28.51 AS.seek 1ml
@14:49:59.26 delay 1 second
@14:50:00.28 AFV.select :holdingCoil
@14:50:00.38 delay 1 second
@14:50:01.41 AS.reconfigure ASfast
@14:50:01.46 AS.seek 0ml
@14:50:28.78 AFV.select :carrierSolution
@14:50:28.89 delay 2 seconds
@14:50:30.91 AS.reconfigure ASconfig
@14:50:30.97 AS.seek 1ml
@14:51:28.73 delay 1 second
@14:51:29.75 AFV.select :holdingCoil
@14:51:29.85 delay 1 second
@14:51:30.88 AS.reconfigure ASfast
@14:51:30.93 AS.seek 0ml
@14:51:58.28 AFV.select :carrierSolution
@14:51:58.39 delay 2 seconds
@14:52:00.41 AS.reconfigure ASconfig
@14:52:00.47 AS.seek 1ml
@14:52:58.21 delay 1 second
@14:52:59.23 AFV.select :holdingCoil
@14:52:59.33 delay 1 second
@14:53:00.36 AS.reconfigure ASfast
@14:53:00.41 AS.seek 0ml
@14:53:27.76 ARV.dial between carrier and air1
@14:53:28.29 ATV.dial :air3
@14:53:29.64 AS.reconfigure ASslow1
@14:53:29.71 AS.seek 0.01ml
@14:53:32.01 delay 2 seconds
@14:53:34.03 ATV.dial :bleach
@14:53:34.68 AS.seek 0.81ml
@14:56:15.81 delay 3 seconds
@14:56:18.83 ATV.dial :air3
@14:56:19.43 delay 3 seconds
@14:56:22.50 AS.seek 0.835ml
@14:56:27.79 delay 3 seconds
@14:56:30.81 ATV.dial :bleach
@14:56:31.46 AS.seek 0.82ml
@14:56:34.75 delay 3 seconds
@14:56:37.77 ATV.dial PCR
@14:56:38.18 AS.reconfigure ASconfig
@14:56:38.24 AS.seek 0ml
@14:57:25.65 PCR.program :bleachProfile
@14:57:25.72 PCR program should complete in < 2:45
@14:57:25.73 PCR.run 10:00
@15:00:21.51 PCR.off
@15:00:21.56 AFV.select :carrierSolution
@15:00:21.67 delay 2 seconds
@15:00:23.70 AS.seek 1ml
@15:01:21.43 delay 1 second
@15:01:22.45 AFV.select :holdingCoil
@15:01:22.55 delay 1 second
@15:01:23.57 AS.reconfigure ASfast
@15:01:23.63 AS.seek 0ml
@15:01:50.96 delay 3 seconds
@15:01:53.98 AFV.select :carrierSolution
@15:01:54.08 delay 2 seconds
@15:01:56.10 AS.reconfigure ASconfig
@15:01:56.20 AS.seek 1ml
@15:02:54.00 delay 1 second
@15:02:55.02 AFV.select :holdingCoil
@15:02:55.12 delay 1 second
@15:02:56.15 AS.reconfigure ASfast
@15:02:56.20 AS.seek 0ml
@15:03:23.53 delay 3 seconds
@15:03:26.55 AFV.select :carrierSolution
@15:03:26.65 delay 2 seconds
@15:03:28.67 AS.reconfigure ASconfig
@15:03:28.74 AS.seek 1ml
@15:04:26.48 delay 1 second
@15:04:27.50 AFV.select :holdingCoil
@15:04:27.60 delay 1 second
@15:04:28.62 AS.reconfigure ASfast
@15:04:28.68 AS.seek 0ml
@15:04:56.01 delay 3 seconds
@15:04:59.03 AFV.select :carrierSolution
@15:04:59.13 delay 2 seconds
@15:05:01.15 AS.reconfigure ASconfig
@15:05:01.22 AS.seek 1ml
@15:05:58.96 delay 1 second
@15:05:59.98 AFV.select :holdingCoil
@15:06:00.08 delay 1 second
@15:06:01.46 AS.reconfigure ASfast
@15:06:01.58 AS.seek 0ml
@15:06:28.93 delay 3 seconds
@15:06:31.95 AFV.select :carrierSolution
@15:06:32.05 delay 2 seconds
@15:06:34.08 AS.reconfigure ASconfig
@15:06:34.14 AS.seek 1ml
@15:07:31.92 delay 1 second
@15:07:32.94 AFV.select :holdingCoil
@15:07:33.04 delay 1 second
@15:07:34.07 AS.reconfigure ASnorm
@15:07:34.12 AS.seek 0ml
@15:08:24.83 Setting up PCR for ACYPP2 -- writing /var/log/esp/ACYPP221apr2215h.pcr
@15:08:24.85 Building PCR Slug from ASV with MM1 mix, PP2 primer
@15:08:24.87 Priming with carrier
@15:08:24.89 ATV.dial ARV
@15:08:25.54 ARV.dial :carrier
@15:08:25.90 AS.seek 0.5ml
@15:08:51.50 AFV.select :carrierSolution
@15:08:51.62 delay 2 seconds
@15:08:53.65 AS.reconfigure ASconfig
@15:08:53.70 AS.seek 1ml
@15:09:22.73 delay 1 second
@15:09:23.75 AFV.select :holdingCoil
@15:09:23.85 delay 1 second
@15:09:24.87 ATV.dial :waste
@15:09:26.65 AS.reconfigure ASnorm
@15:09:26.71 AS.seek 0ml
@15:10:17.40 AFV.select :carrierSolution
@15:10:17.52 delay 2 seconds
@15:10:19.55 AS.reconfigure ASconfig
@15:10:19.60 AS.seek 0.5ml
@15:10:48.64 delay 1 second
@15:10:49.66 AFV.select :holdingCoil
@15:10:49.77 Building Slug
@15:10:49.79 ATV.dial :ARV
@15:10:51.54 delay 2 seconds
@15:10:53.56 ARV.dial :air1
@15:10:53.97 delay 2 seconds
@15:10:56.04 AS.reconfigure ASslow2
@15:10:56.10 AS.seek 0.506ml
@15:11:02.35 delay 2 seconds
@15:11:04.37 ARV.dial :MM1
@15:11:05.01 delay 2 seconds
@15:11:07.03 Adding MM1
@15:11:07.11 AS.seek 0.524ml
@15:11:25.45 delay 25 seconds
@15:11:50.47 ARV.dial :PP2
@15:11:50.89 Adding PP2
@15:11:50.90 delay 2 seconds
@15:11:52.98 AS.seek 0.53ml
@15:11:59.28 delay 10 seconds
@15:12:09.31 ARV.dial :air2
@15:12:09.75 adding ARV air
@15:12:09.76 delay 2 seconds
@15:12:11.84 AS.seek 0.536ml
@15:12:18.45 delay 5 seconds
@15:12:23.47 ARV.dial :carrier
@15:12:24.81 delay 7 seconds
@15:12:31.89 AS.seek 0.594ml
@15:13:30.53 delay 5 seconds
@15:13:35.55 ATV.dial :ASV
@15:13:35.96 delay 2 seconds
@15:13:38.03 AS.seek 0.6ml
@15:13:44.60 delay 4 seconds
@15:13:48.62 ATV.dial ARV
@15:13:49.31 delay 2 seconds
@15:13:51.33 ARV.dial :carrier
@15:13:51.34 delay 2 seconds
@15:13:53.42 AS.seek 0.65ml
@15:14:44.04 delay 4 seconds
@15:14:48.06 ATV.dial PCR
@15:14:48.71 ASV.dial :waste1
@15:14:48.72 ARV.dial :air1
@15:14:49.15 Positioning Slug by 0.324ml
@15:14:49.24 AS.seek 0.326ml
@15:20:15.37 delay 5 seconds
@15:20:20.39 ATV.dial ARV
@15:20:21.21 ASV.dial :PCRlock
@15:20:21.60 delay 5 seconds
@15:20:26.62 Running ACYPP2 Profile60CIQ2 thermocycling
@15:20:26.64 <PCR> PCR.program Profile60CIQ2
@15:20:26.75 Profile60CIQ2 should complete in < 1:47:50
@15:20:26.76 PCR.run 2:19:47.5
@16:49:47.36 PCR.off
@16:49:47.98 <MFBPCR> Running ACYPP2 MeltProfile60 thermocycling
@16:49:48.00 <PCR> PCR.program MeltProfile60
@16:49:48.10 MeltProfile60 should complete in < 11:55
@16:49:48.11 PCR.run 19:53.75
@16:57:53.52 PCR.off
@16:57:54.46 <MFBPCR> PCR for ACYPP2 completed -- closed /var/log/esp/ACYPP221apr2215h.pcr
@16:57:54.53 Cleaning PCR
@16:57:54.54 AFV.select :carrierSolution
@16:57:54.65 delay 2 seconds
@16:57:56.68 AS.reconfigure ASconfig
@16:57:56.73 AS.seek 0.5ml
@16:58:07.07 delay 1 second
@16:58:08.09 AFV.select :holdingCoil
@16:58:08.19 ASV.dial :waste1
@16:58:08.60 ATV.dial :air3
@16:58:09.96 AS.reconfigure ASslow1
@16:58:10.02 AS.seek 0.51ml
@16:58:12.33 delay 2 seconds
@16:58:14.35 ATV.dial :bleach
@16:58:15.01 AS.seek 0.81ml
@16:59:15.66 delay 3 seconds
@16:59:18.68 ATV.dial :air3
@16:59:19.27 delay 3 seconds
@16:59:22.34 AS.seek 0.835ml
@16:59:27.63 delay 3 seconds
@16:59:30.65 ATV.dial :bleach
@16:59:31.29 AS.seek 0.82ml
@16:59:34.72 delay 3 seconds
@16:59:37.74 ARV.dial :waste
@16:59:38.40 delay 3 seconds
@16:59:41.42 ATV.dial :ARV
@16:59:42.34 AS.reconfigure ASconfig
@16:59:42.40 AS.seek 0.47ml
@17:00:02.82 AFV.select :carrierSolution
@17:00:02.93 delay 2 seconds
@17:00:04.96 AS.seek 1ml
@17:00:35.72 delay 1 second
@17:00:36.74 AFV.select :holdingCoil
@17:00:36.84 delay 1 second
@17:00:37.87 AS.reconfigure ASfast
@17:00:37.92 AS.seek 0ml
@17:01:05.26 AFV.select :carrierSolution
@17:01:05.37 delay 2 seconds
@17:01:07.39 AS.reconfigure ASconfig
@17:01:07.45 AS.seek 1ml
@17:02:05.19 delay 1 second
@17:02:06.21 AFV.select :holdingCoil
@17:02:06.31 delay 1 second
@17:02:07.33 AS.reconfigure ASfast
@17:02:07.39 AS.seek 0ml
@17:02:34.74 AFV.select :carrierSolution
@17:02:34.84 delay 2 seconds
@17:02:36.86 AS.reconfigure ASconfig
@17:02:36.92 AS.seek 1ml
@17:03:34.66 delay 1 second
@17:03:35.68 AFV.select :holdingCoil
@17:03:35.78 delay 1 second
@17:03:36.80 AS.reconfigure ASfast
@17:03:36.86 AS.seek 0ml
@17:04:04.19 ARV.dial between carrier and air1
@17:04:04.71 ATV.dial :air3
@17:04:06.06 AS.reconfigure ASslow1
@17:04:06.12 AS.seek 0.01ml
@17:04:08.49 delay 2 seconds
@17:04:10.51 ATV.dial :bleach
@17:04:11.16 AS.seek 0.81ml
@17:06:52.36 delay 3 seconds
@17:06:55.38 ATV.dial :air3
@17:06:55.99 delay 3 seconds
@17:06:59.06 AS.seek 0.835ml
@17:07:04.34 delay 3 seconds
@17:07:07.36 ATV.dial :bleach
@17:07:07.99 AS.seek 0.82ml
@17:07:11.30 delay 3 seconds
@17:07:14.32 ATV.dial PCR
@17:07:14.74 AS.reconfigure ASconfig
@17:07:14.80 AS.seek 0ml
@17:08:02.22 PCR.program :bleachProfile
@17:08:02.29 PCR program should complete in < 2:45
@17:08:02.30 PCR.run 10:00
@17:10:58.50 PCR.off
@17:10:58.54 AFV.select :carrierSolution
@17:10:58.65 delay 2 seconds
@17:11:00.68 AS.seek 1ml
@17:11:58.43 delay 1 second
@17:11:59.45 AFV.select :holdingCoil
@17:11:59.55 delay 1 second
@17:12:00.58 AS.reconfigure ASfast
@17:12:00.63 AS.seek 0ml
@17:12:27.97 delay 3 seconds
@17:12:30.99 AFV.select :carrierSolution
@17:12:31.09 delay 2 seconds
@17:12:33.11 AS.reconfigure ASconfig
@17:12:33.18 AS.seek 1ml
@17:13:30.93 delay 1 second
@17:13:31.95 AFV.select :holdingCoil
@17:13:32.05 delay 1 second
@17:13:33.07 AS.reconfigure ASfast
@17:13:33.13 AS.seek 0ml
@17:14:00.47 delay 3 seconds
@17:14:03.49 AFV.select :carrierSolution
@17:14:03.59 delay 2 seconds
@17:14:05.61 AS.reconfigure ASconfig
@17:14:05.68 AS.seek 1ml
@17:15:03.44 delay 1 second
@17:15:04.46 AFV.select :holdingCoil
@17:15:04.56 delay 1 second
@17:15:05.59 AS.reconfigure ASfast
@17:15:05.64 AS.seek 0ml
@17:15:32.97 delay 3 seconds
@17:15:35.99 AFV.select :carrierSolution
@17:15:36.09 delay 2 seconds
@17:15:38.11 AS.reconfigure ASconfig
@17:15:38.18 AS.seek 1ml
@17:16:35.93 delay 1 second
@17:16:36.95 AFV.select :holdingCoil
@17:16:37.05 delay 1 second
@17:16:38.07 AS.reconfigure ASfast
@17:16:38.13 AS.seek 0ml
@17:17:05.47 delay 3 seconds
@17:17:08.49 AFV.select :carrierSolution
@17:17:08.59 delay 2 seconds
@17:17:10.61 AS.reconfigure ASconfig
@17:17:10.68 AS.seek 1ml
@17:18:08.44 delay 1 second
@17:18:09.46 AFV.select :holdingCoil
@17:18:09.56 delay 1 second
@17:18:10.59 AS.reconfigure ASnorm
@17:18:10.64 AS.seek 0ml
@17:19:01.34 Setting up PCR for AOPP1 -- writing /var/log/esp/AOPP121apr2217h.pcr
@17:19:01.36 Building PCR Slug from ASV with MM1 mix, PP1 primer
@17:19:01.38 Priming with carrier
@17:19:01.40 ATV.dial ARV
@17:19:02.05 ARV.dial :carrier
@17:19:02.41 AS.seek 0.5ml
@17:19:27.99 AFV.select :carrierSolution
@17:19:28.11 delay 2 seconds
@17:19:30.14 AS.reconfigure ASconfig
@17:19:30.19 AS.seek 1ml
@17:19:59.22 delay 1 second
@17:20:00.24 AFV.select :holdingCoil
@17:20:00.34 delay 1 second
@17:20:01.36 ATV.dial :waste
@17:20:03.15 AS.reconfigure ASnorm
@17:20:03.21 AS.seek 0ml
@17:20:53.93 AFV.select :carrierSolution
@17:20:54.04 delay 2 seconds
@17:20:56.07 AS.reconfigure ASconfig
@17:20:56.12 AS.seek 0.5ml
@17:21:25.15 delay 1 second
@17:21:26.17 AFV.select :holdingCoil
@17:21:26.27 Building Slug
@17:21:26.29 ATV.dial :ARV
@17:21:28.04 delay 2 seconds
@17:21:30.06 ARV.dial :air1
@17:21:30.55 delay 2 seconds
@17:21:32.63 AS.reconfigure ASslow2
@17:21:32.69 AS.seek 0.506ml
@17:21:38.99 delay 2 seconds
@17:21:41.41 ARV.dial :MM1
@17:21:42.07 delay 2 seconds
@17:21:44.09 Adding MM1
@17:21:44.18 AS.seek 0.524ml
@17:22:02.61 delay 25 seconds
@17:22:27.63 ARV.dial :PP1
@17:22:28.04 Adding PP1
@17:22:28.05 delay 2 seconds
@17:22:30.13 AS.seek 0.53ml
@17:22:36.74 delay 10 seconds
@17:22:46.77 ARV.dial :air1
@17:22:47.19 adding ARV air
@17:22:47.20 delay 2 seconds
@17:22:49.28 AS.seek 0.536ml
@17:22:55.96 delay 5 seconds
@17:23:00.98 ARV.dial :carrier
@17:23:01.41 delay 7 seconds
@17:23:08.49 AS.seek 0.594ml
@17:24:07.04 delay 5 seconds
@17:24:12.06 ATV.dial :ASV
@17:24:12.47 delay 2 seconds
@17:24:14.54 AS.seek 0.6ml
@17:24:21.08 delay 4 seconds
@17:24:25.10 ATV.dial ARV
@17:24:25.69 delay 2 seconds
@17:24:27.71 ARV.dial :carrier
@17:24:27.72 delay 2 seconds
@17:24:29.80 AS.seek 0.65ml
@17:25:20.21 delay 4 seconds
@17:25:24.23 ATV.dial PCR
@17:25:24.86 ASV.dial :waste1
@17:25:24.87 ARV.dial :air1
@17:25:25.30 Positioning Slug by 0.324ml
@17:25:25.39 AS.seek 0.326ml
@17:30:51.57 delay 5 seconds
@17:30:56.59 ATV.dial ARV
@17:30:57.41 ASV.dial :PCRlock
@17:30:57.82 delay 5 seconds
@17:31:02.84 Running AOPP1 Profile60CIQ1 thermocycling
@17:31:02.85 <PCR> PCR.program Profile60CIQ1
@17:31:02.97 Profile60CIQ1 should complete in < 1:56:15
@17:31:02.98 PCR.run 2:30:18.75
@19:10:53.07 PCR.off
@19:10:53.64 <MFBPCR> Running AOPP1 MeltProfile60 thermocycling
@19:10:53.65 <PCR> PCR.program MeltProfile60
@19:10:53.76 MeltProfile60 should complete in < 11:55
@19:10:53.77 PCR.run 19:53.75
@19:18:56.05 PCR.off
@19:18:56.98 <MFBPCR> PCR for AOPP1 completed -- closed /var/log/esp/AOPP121apr2217h.pcr
@19:18:57.05 Cleaning PCR
@19:18:57.06 AFV.select :carrierSolution
@19:18:57.18 delay 2 seconds
@19:18:59.21 AS.reconfigure ASconfig
@19:18:59.26 AS.seek 0.5ml
@19:19:09.58 delay 1 second
@19:19:10.60 AFV.select :holdingCoil
@19:19:10.70 ASV.dial :waste1
@19:19:11.11 ATV.dial :air3
@19:19:12.47 AS.reconfigure ASslow1
@19:19:12.53 AS.seek 0.51ml
@19:19:14.82 delay 2 seconds
@19:19:16.84 ATV.dial :bleach
@19:19:17.47 AS.seek 0.81ml
@19:20:18.06 delay 3 seconds
@19:20:21.08 ATV.dial :air3
@19:20:21.67 delay 3 seconds
@19:20:24.74 AS.seek 0.835ml
@19:20:30.03 delay 3 seconds
@19:20:33.05 ATV.dial :bleach
@19:20:33.70 AS.seek 0.82ml
@19:20:37.12 delay 3 seconds
@19:20:40.14 ARV.dial :waste
@19:20:40.78 delay 3 seconds
@19:20:43.80 ATV.dial :ARV
@19:20:44.72 AS.reconfigure ASconfig
@19:20:44.78 AS.seek 0.47ml
@19:21:05.20 AFV.select :carrierSolution
@19:21:05.31 delay 2 seconds
@19:21:07.34 AS.seek 1ml
@19:21:38.08 delay 1 second
@19:21:39.10 AFV.select :holdingCoil
@19:21:39.20 delay 1 second
@19:21:40.22 AS.reconfigure ASfast
@19:21:40.28 AS.seek 0ml
@19:22:07.62 AFV.select :carrierSolution
@19:22:07.73 delay 2 seconds
@19:22:09.75 AS.reconfigure ASconfig
@19:22:09.81 AS.seek 1ml
@19:23:07.57 delay 1 second
@19:23:08.60 AFV.select :holdingCoil
@19:23:09.11 delay 1 second
@19:23:10.14 AS.reconfigure ASfast
@19:23:10.19 AS.seek 0ml
@19:23:37.53 AFV.select :carrierSolution
@19:23:37.64 delay 2 seconds
@19:23:39.67 AS.reconfigure ASconfig
@19:23:39.72 AS.seek 1ml
@19:24:37.47 delay 1 second
@19:24:38.49 AFV.select :holdingCoil
@19:24:38.59 delay 1 second
@19:24:39.62 AS.reconfigure ASfast
@19:24:39.67 AS.seek 0ml
@19:25:06.98 ARV.dial between carrier and air1
@19:25:07.52 ATV.dial :air3
@19:25:08.87 AS.reconfigure ASslow1
@19:25:08.93 AS.seek 0.01ml
@19:25:11.31 delay 2 seconds
@19:25:13.33 ATV.dial :bleach
@19:25:13.98 AS.seek 0.81ml
@19:27:55.11 delay 3 seconds
@19:27:58.13 ATV.dial :air3
@19:27:58.72 delay 3 seconds
@19:28:01.79 AS.seek 0.835ml
@19:28:07.10 delay 3 seconds
@19:28:10.12 ATV.dial :bleach
@19:28:10.76 AS.seek 0.82ml
@19:28:14.02 delay 3 seconds
@19:28:17.04 ATV.dial PCR
@19:28:17.45 AS.reconfigure ASconfig
@19:28:17.51 AS.seek 0ml
@19:29:04.94 PCR.program :bleachProfile
@19:29:05.01 PCR program should complete in < 2:45
@19:29:05.02 PCR.run 10:00
@19:32:00.81 PCR.off
@19:32:00.86 AFV.select :carrierSolution
@19:32:00.97 delay 2 seconds
@19:32:03.00 AS.seek 1ml
@19:33:00.80 delay 1 second
@19:33:01.82 AFV.select :holdingCoil
@19:33:01.92 delay 1 second
@19:33:02.95 AS.reconfigure ASfast
@19:33:03.00 AS.seek 0ml
@19:33:30.34 delay 3 seconds
@19:33:33.36 AFV.select :carrierSolution
@19:33:33.46 delay 2 seconds
@19:33:35.48 AS.reconfigure ASconfig
@19:33:35.54 AS.seek 1ml
@19:34:33.33 delay 1 second
@19:34:34.35 AFV.select :holdingCoil
@19:34:34.45 delay 1 second
@19:34:35.47 AS.reconfigure ASfast
@19:34:35.53 AS.seek 0ml
@19:35:02.86 delay 3 seconds
@19:35:05.88 AFV.select :carrierSolution
@19:35:05.98 delay 2 seconds
@19:35:08.00 AS.reconfigure ASconfig
@19:35:08.07 AS.seek 1ml
@19:36:05.81 delay 1 second
@19:36:06.83 AFV.select :holdingCoil
@19:36:06.93 delay 1 second
@19:36:07.96 AS.reconfigure ASfast
@19:36:08.01 AS.seek 0ml
@19:36:35.33 delay 3 seconds
@19:36:38.35 AFV.select :carrierSolution
@19:36:38.45 delay 2 seconds
@19:36:40.98 AS.reconfigure ASconfig
@19:36:41.06 AS.seek 1ml
@19:37:38.86 delay 1 second
@19:37:39.88 AFV.select :holdingCoil
@19:37:39.98 delay 1 second
@19:37:41.01 AS.reconfigure ASfast
@19:37:41.06 AS.seek 0ml
@19:38:08.41 delay 3 seconds
@19:38:11.43 AFV.select :carrierSolution
@19:38:11.53 delay 2 seconds
@19:38:13.56 AS.reconfigure ASconfig
@19:38:13.61 AS.seek 1ml
@19:39:11.39 delay 1 second
@19:39:12.41 AFV.select :holdingCoil
@19:39:12.51 delay 1 second
@19:39:13.54 AS.reconfigure ASnorm
@19:39:13.59 AS.seek 0ml
@19:40:04.33 Setting up PCR for AARPP4 -- writing /var/log/esp/AARPP421apr2219h.pcr
@19:40:04.35 Building PCR Slug from ASV with MM2 mix, PP4 primer
@19:40:04.37 Priming with carrier
@19:40:04.39 ATV.dial ARV
@19:40:05.05 ARV.dial :carrier
@19:40:05.40 AS.seek 0.5ml
@19:40:30.94 AFV.select :carrierSolution
@19:40:31.06 delay 2 seconds
@19:40:33.09 AS.reconfigure ASconfig
@19:40:33.14 AS.seek 1ml
@19:41:02.16 delay 1 second
@19:41:03.18 AFV.select :holdingCoil
@19:41:03.28 delay 1 second
@19:41:04.30 ATV.dial :waste
@19:41:06.08 AS.reconfigure ASnorm
@19:41:06.14 AS.seek 0ml
@19:41:56.86 AFV.select :carrierSolution
@19:41:56.97 delay 2 seconds
@19:41:59.00 AS.reconfigure ASconfig
@19:41:59.05 AS.seek 0.5ml
@19:42:28.09 delay 1 second
@19:42:29.11 AFV.select :holdingCoil
@19:42:29.21 Building Slug
@19:42:29.22 ATV.dial :ARV
@19:42:30.97 delay 2 seconds
@19:42:32.99 ARV.dial :air1
@19:42:33.40 delay 2 seconds
@19:42:35.47 AS.reconfigure ASslow2
@19:42:35.53 AS.seek 0.506ml
@19:42:41.78 delay 2 seconds
@19:42:43.80 ARV.dial :MM2
@19:42:45.41 delay 2 seconds
@19:42:47.43 Adding MM2
@19:42:47.51 AS.seek 0.524ml
@19:43:05.95 delay 25 seconds
@19:43:30.97 ARV.dial :PP4
@19:43:31.39 Adding PP4
@19:43:31.40 delay 2 seconds
@19:43:33.48 AS.seek 0.53ml
@19:43:39.92 delay 10 seconds
@19:43:49.95 ARV.dial :air3
@19:43:50.37 adding ARV air
@19:43:50.38 delay 2 seconds
@19:43:52.46 AS.seek 0.536ml
@19:43:58.94 delay 5 seconds
@19:44:03.96 ARV.dial :carrier
@19:44:05.30 delay 7 seconds
@19:44:12.38 AS.seek 0.594ml
@19:45:11.36 delay 5 seconds
@19:45:16.38 ATV.dial :ASV
@19:45:16.81 delay 2 seconds
@19:45:18.88 AS.seek 0.6ml
@19:45:25.12 delay 4 seconds
@19:45:29.14 ATV.dial ARV
@19:45:29.73 delay 2 seconds
@19:45:31.75 ARV.dial :carrier
@19:45:31.76 delay 2 seconds
@19:45:33.84 AS.seek 0.65ml
@19:46:24.51 delay 4 seconds
@19:46:28.53 ATV.dial PCR
@19:46:29.17 ASV.dial :waste1
@19:46:29.18 ARV.dial :air1
@19:46:29.60 Positioning Slug by 0.324ml
@19:46:29.69 AS.seek 0.326ml
@19:51:56.09 delay 5 seconds
@19:52:01.11 ATV.dial ARV
@19:52:01.97 ASV.dial :PCRlock
@19:52:02.37 delay 5 seconds
@19:52:07.39 Running AARPP4 Profile58CIQ2 thermocycling
@19:52:07.41 <PCR> PCR.program Profile58CIQ2
@19:52:07.52 Profile58CIQ2 should complete in < 1:36:15
@19:52:07.53 PCR.run 2:05:18.75
@21:13:42.06 PCR.off
@21:13:42.62 <MFBPCR> Running AARPP4 MeltProfile58 thermocycling
@21:13:42.64 <PCR> PCR.program MeltProfile58
@21:13:42.74 MeltProfile58 should complete in < 12:33
@21:13:42.75 PCR.run 20:41.25
@21:21:48.97 PCR.off
@21:21:49.94 <MFBPCR> PCR for AARPP4 completed -- closed /var/log/esp/AARPP421apr2219h.pcr
@21:21:50.01 Cleaning PCR
@21:21:50.02 AFV.select :carrierSolution
@21:21:50.14 delay 2 seconds
@21:21:52.17 AS.reconfigure ASconfig
@21:21:52.22 AS.seek 0.5ml
@21:22:02.56 delay 1 second
@21:22:03.58 AFV.select :holdingCoil
@21:22:03.68 ASV.dial :waste1
@21:22:04.09 ATV.dial :air3
@21:22:05.45 AS.reconfigure ASslow1
@21:22:05.51 AS.seek 0.51ml
@21:22:07.83 delay 2 seconds
@21:22:09.85 ATV.dial :bleach
@21:22:10.49 AS.seek 0.81ml
@21:23:11.15 delay 3 seconds
@21:23:14.17 ATV.dial :air3
@21:23:14.76 delay 3 seconds
@21:23:17.83 AS.seek 0.835ml
@21:23:23.12 delay 3 seconds
@21:23:26.14 ATV.dial :bleach
@21:23:26.78 AS.seek 0.82ml
@21:23:30.20 delay 3 seconds
@21:23:33.22 ARV.dial :waste
@21:23:33.86 delay 3 seconds
@21:23:36.88 ATV.dial :ARV
@21:23:37.81 AS.reconfigure ASconfig
@21:23:37.87 AS.seek 0.47ml
@21:23:58.29 AFV.select :carrierSolution
@21:23:58.40 delay 2 seconds
@21:24:00.43 AS.seek 1ml
@21:24:31.15 delay 1 second
@21:24:32.17 AFV.select :holdingCoil
@21:24:32.27 delay 1 second
@21:24:33.30 AS.reconfigure ASfast
@21:24:33.35 AS.seek 0ml
@21:25:00.68 AFV.select :carrierSolution
@21:25:00.79 delay 2 seconds
@21:25:02.81 AS.reconfigure ASconfig
@21:25:02.87 AS.seek 1ml
@21:26:00.67 delay 1 second
@21:26:01.69 AFV.select :holdingCoil
@21:26:01.79 delay 1 second
@21:26:02.81 AS.reconfigure ASfast
@21:26:02.87 AS.seek 0ml
@21:26:30.21 AFV.select :carrierSolution
@21:26:30.32 delay 2 seconds
@21:26:32.35 AS.reconfigure ASconfig
@21:26:32.40 AS.seek 1ml
@21:27:30.15 delay 1 second
@21:27:31.17 AFV.select :holdingCoil
@21:27:31.27 delay 1 second
@21:27:32.29 AS.reconfigure ASfast
@21:27:32.35 AS.seek 0ml
@21:27:59.67 ARV.dial between carrier and air1
@21:28:00.20 ATV.dial :air3
@21:28:01.56 AS.reconfigure ASslow1
@21:28:01.62 AS.seek 0.01ml
@21:28:04.04 delay 2 seconds
@21:28:06.06 ATV.dial :bleach
@21:28:06.70 AS.seek 0.81ml
@21:30:47.85 delay 3 seconds
@21:30:50.87 ATV.dial :air3
@21:30:51.46 delay 3 seconds
@21:30:54.53 AS.seek 0.835ml
@21:30:59.83 delay 3 seconds
@21:31:02.85 ATV.dial :bleach
@21:31:03.48 AS.seek 0.82ml
@21:31:06.79 delay 3 seconds
@21:31:09.81 ATV.dial PCR
@21:31:10.23 AS.reconfigure ASconfig
@21:31:10.29 AS.seek 0ml
@21:31:57.70 PCR.program :bleachProfile
@21:31:57.76 PCR program should complete in < 2:45
@21:31:57.77 PCR.run 10:00
@21:34:53.98 PCR.off
@21:34:54.02 AFV.select :carrierSolution
@21:34:54.13 delay 2 seconds
@21:34:56.16 AS.seek 1ml
@21:35:53.95 delay 1 second
@21:35:54.97 AFV.select :holdingCoil
@21:35:55.07 delay 1 second
@21:35:56.10 AS.reconfigure ASfast
@21:35:56.15 AS.seek 0ml
@21:36:23.49 delay 3 seconds
@21:36:26.51 AFV.select :carrierSolution
@21:36:26.61 delay 2 seconds
@21:36:28.63 AS.reconfigure ASconfig
@21:36:28.70 AS.seek 1ml
@21:37:26.43 delay 1 second
@21:37:27.45 AFV.select :holdingCoil
@21:37:27.55 delay 1 second
@21:37:28.57 AS.reconfigure ASfast
@21:37:28.63 AS.seek 0ml
@21:37:55.96 delay 3 seconds
@21:37:58.98 AFV.select :carrierSolution
@21:37:59.08 delay 2 seconds
@21:38:01.10 AS.reconfigure ASconfig
@21:38:01.17 AS.seek 1ml
@21:38:58.92 delay 1 second
@21:38:59.94 AFV.select :holdingCoil
@21:39:00.04 delay 1 second
@21:39:01.07 AS.reconfigure ASfast
@21:39:01.12 AS.seek 0ml
@21:39:28.45 delay 3 seconds
@21:39:31.47 AFV.select :carrierSolution
@21:39:31.57 delay 2 seconds
@21:39:33.59 AS.reconfigure ASconfig
@21:39:33.65 AS.seek 1ml
@21:40:31.42 delay 1 second
@21:40:32.44 AFV.select :holdingCoil
@21:40:32.54 delay 1 second
@21:40:33.56 AS.reconfigure ASfast
@21:40:33.62 AS.seek 0ml
@21:41:00.96 delay 3 seconds
@21:41:03.98 AFV.select :carrierSolution
@21:41:04.08 delay 2 seconds
@21:41:06.10 AS.reconfigure ASconfig
@21:41:06.17 AS.seek 1ml
@21:42:03.92 delay 1 second
@21:42:04.94 AFV.select :holdingCoil
@21:42:05.04 delay 1 second
@21:42:06.07 AS.reconfigure ASnorm
@21:42:06.12 AS.seek 0ml
@21:42:56.87 Setting up PCR for AALPP3 -- writing /var/log/esp/AALPP321apr2221h.pcr
@21:42:56.89 Building PCR Slug from ASV with MM2 mix, PP3 primer
@21:42:56.91 Priming with carrier
@21:42:56.93 ATV.dial ARV
@21:42:57.59 ARV.dial :carrier
@21:42:57.95 AS.seek 0.5ml
@21:43:23.51 AFV.select :carrierSolution
@21:43:23.62 delay 2 seconds
@21:43:25.65 AS.reconfigure ASconfig
@21:43:25.71 AS.seek 1ml
@21:43:54.74 delay 1 second
@21:43:55.76 AFV.select :holdingCoil
@21:43:55.86 delay 1 second
@21:43:56.88 ATV.dial :waste
@21:43:58.67 AS.reconfigure ASnorm
@21:43:58.74 AS.seek 0ml
@21:44:49.45 AFV.select :carrierSolution
@21:44:49.56 delay 2 seconds
@21:44:51.59 AS.reconfigure ASconfig
@21:44:51.64 AS.seek 0.5ml
@21:45:20.68 delay 1 second
@21:45:21.70 AFV.select :holdingCoil
@21:45:21.80 Building Slug
@21:45:21.81 ATV.dial :ARV
@21:45:23.56 delay 2 seconds
@21:45:25.58 ARV.dial :air1
@21:45:25.99 delay 2 seconds
@21:45:28.06 AS.reconfigure ASslow2
@21:45:28.12 AS.seek 0.506ml
@21:45:34.40 delay 2 seconds
@21:45:36.42 ARV.dial :MM2
@21:45:38.01 delay 2 seconds
@21:45:40.03 Adding MM2
@21:45:40.11 AS.seek 0.524ml
@21:45:58.42 delay 25 seconds
@21:46:23.44 ARV.dial :PP3
@21:46:23.84 Adding PP3
@21:46:23.85 delay 2 seconds
@21:46:25.93 AS.seek 0.53ml
@21:46:32.46 delay 10 seconds
@21:46:42.49 ARV.dial :air2
@21:46:42.90 adding ARV air
@21:46:42.91 delay 2 seconds
@21:46:44.99 AS.seek 0.536ml
@21:46:51.35 delay 5 seconds
@21:46:56.78 ARV.dial :carrier
@21:46:58.14 delay 7 seconds
@21:47:05.22 AS.seek 0.594ml
@21:48:04.01 delay 5 seconds
@21:48:09.03 ATV.dial :ASV
@21:48:09.46 delay 2 seconds
@21:48:11.53 AS.seek 0.6ml
@21:48:18.12 delay 4 seconds
@21:48:22.14 ATV.dial ARV
@21:48:22.73 delay 2 seconds
@21:48:24.75 ARV.dial :carrier
@21:48:24.76 delay 2 seconds
@21:48:26.84 AS.seek 0.65ml
@21:49:17.64 delay 4 seconds
@21:49:21.66 ATV.dial PCR
@21:49:22.32 ASV.dial :waste1
@21:49:22.33 ARV.dial :air1
@21:49:22.77 Positioning Slug by 0.324ml
@21:49:22.86 AS.seek 0.326ml
@21:54:49.09 delay 5 seconds
@21:54:54.11 ATV.dial ARV
@21:54:54.95 ASV.dial :PCRlock
@21:54:55.35 delay 5 seconds
@21:55:00.37 Running AALPP3 Profile58CIQ2 thermocycling
@21:55:00.38 <PCR> PCR.program Profile58CIQ2
@21:55:00.51 Profile58CIQ2 should complete in < 1:36:15
@21:55:00.52 PCR.run 2:05:18.75
@23:16:31.27 PCR.off
@23:16:31.83 <MFBPCR> Running AALPP3 MeltProfile58 thermocycling
@23:16:31.84 <PCR> PCR.program MeltProfile58
@23:16:31.95 MeltProfile58 should complete in < 12:33
@23:16:31.96 PCR.run 20:41.25
@23:24:32.19 PCR.off
@23:24:33.17 <MFBPCR> PCR for AALPP3 completed -- closed /var/log/esp/AALPP321apr2221h.pcr
@23:24:33.24 Cleaning PCR
@23:24:33.25 AFV.select :carrierSolution
@23:24:33.37 delay 2 seconds
@23:24:35.40 AS.reconfigure ASconfig
@23:24:35.45 AS.seek 0.5ml
@23:24:45.73 delay 1 second
@23:24:46.75 AFV.select :holdingCoil
@23:24:46.85 ASV.dial :waste1
@23:24:47.24 ATV.dial :air3
@23:24:48.59 AS.reconfigure ASslow1
@23:24:48.65 AS.seek 0.51ml
@23:24:50.96 delay 2 seconds
@23:24:52.98 ATV.dial :bleach
@23:24:53.78 AS.seek 0.81ml
@23:25:54.46 delay 3 seconds
@23:25:57.48 ATV.dial :air3
@23:25:58.07 delay 3 seconds
@23:26:01.14 AS.seek 0.835ml
@23:26:06.49 delay 3 seconds
@23:26:09.51 ATV.dial :bleach
@23:26:10.15 AS.seek 0.82ml
@23:26:13.48 delay 3 seconds
@23:26:16.50 ARV.dial :waste
@23:26:17.15 delay 3 seconds
@23:26:20.17 ATV.dial :ARV
@23:26:21.10 AS.reconfigure ASconfig
@23:26:21.16 AS.seek 0.47ml
@23:26:41.55 AFV.select :carrierSolution
@23:26:41.66 delay 2 seconds
@23:26:43.69 AS.seek 1ml
@23:27:14.48 delay 1 second
@23:27:15.50 AFV.select :holdingCoil
@23:27:15.60 delay 1 second
@23:27:16.63 AS.reconfigure ASfast
@23:27:16.68 AS.seek 0ml
@23:27:44.01 AFV.select :carrierSolution
@23:27:44.12 delay 2 seconds
@23:27:46.14 AS.reconfigure ASconfig
@23:27:46.20 AS.seek 1ml
@23:28:43.95 delay 1 second
@23:28:44.97 AFV.select :holdingCoil
@23:28:45.07 delay 1 second
@23:28:46.10 AS.reconfigure ASfast
@23:28:46.15 AS.seek 0ml
@23:29:13.49 AFV.select :carrierSolution
@23:29:13.60 delay 2 seconds
@23:29:15.62 AS.reconfigure ASconfig
@23:29:16.09 AS.seek 1ml
@23:30:13.84 delay 1 second
@23:30:14.86 AFV.select :holdingCoil
@23:30:14.96 delay 1 second
@23:30:15.99 AS.reconfigure ASfast
@23:30:16.04 AS.seek 0ml
@23:30:43.38 ARV.dial between carrier and air1
@23:30:43.91 ATV.dial :air3
@23:30:45.29 AS.reconfigure ASslow1
@23:30:45.35 AS.seek 0.01ml
@23:30:47.77 delay 2 seconds
@23:30:49.79 ATV.dial :bleach
@23:30:50.46 AS.seek 0.81ml
@23:33:31.62 delay 3 seconds
@23:33:34.64 ATV.dial :air3
@23:33:35.23 delay 3 seconds
@23:33:38.30 AS.seek 0.835ml
@23:33:43.59 delay 3 seconds
@23:33:46.61 ATV.dial :bleach
@23:33:47.26 AS.seek 0.82ml
@23:33:50.55 delay 3 seconds
@23:33:53.57 ATV.dial PCR
@23:33:53.99 AS.reconfigure ASconfig
@23:33:54.05 AS.seek 0ml
@23:34:41.46 PCR.program :bleachProfile
@23:34:41.53 PCR program should complete in < 2:45
@23:34:41.54 PCR.run 10:00
@23:37:37.33 PCR.off
@23:37:37.37 AFV.select :carrierSolution
@23:37:37.48 delay 2 seconds
@23:37:39.51 AS.seek 1ml
@23:38:37.24 delay 1 second
@23:38:38.26 AFV.select :holdingCoil
@23:38:38.36 delay 1 second
@23:38:39.38 AS.reconfigure ASfast
@23:38:39.44 AS.seek 0ml
@23:39:06.77 delay 3 seconds
@23:39:09.79 AFV.select :carrierSolution
@23:39:09.89 delay 2 seconds
@23:39:11.91 AS.reconfigure ASconfig
@23:39:11.98 AS.seek 1ml
@23:40:09.74 delay 1 second
@23:40:10.76 AFV.select :holdingCoil
@23:40:10.86 delay 1 second
@23:40:11.89 AS.reconfigure ASfast
@23:40:11.94 AS.seek 0ml
@23:40:39.27 delay 3 seconds
@23:40:42.29 AFV.select :carrierSolution
@23:40:42.39 delay 2 seconds
@23:40:44.41 AS.reconfigure ASconfig
@23:40:44.48 AS.seek 1ml
@23:41:42.26 delay 1 second
@23:41:43.28 AFV.select :holdingCoil
@23:41:43.38 delay 1 second
@23:41:44.40 AS.reconfigure ASfast
@23:41:44.46 AS.seek 0ml
@23:42:11.80 delay 3 seconds
@23:42:14.82 AFV.select :carrierSolution
@23:42:14.93 delay 2 seconds
@23:42:16.95 AS.reconfigure ASconfig
@23:42:17.02 AS.seek 1ml
@23:43:14.76 delay 1 second
@23:43:15.78 AFV.select :holdingCoil
@23:43:15.88 delay 1 second
@23:43:16.91 AS.reconfigure ASfast
@23:43:17.37 AS.seek 0ml
@23:43:44.71 delay 3 seconds
@23:43:47.73 AFV.select :carrierSolution
@23:43:47.83 delay 2 seconds
@23:43:49.85 AS.reconfigure ASconfig
@23:43:49.92 AS.seek 1ml
@23:44:47.66 delay 1 second
@23:44:48.68 AFV.select :holdingCoil
@23:44:48.78 delay 1 second
@23:44:49.81 AS.reconfigure ASnorm
@23:44:49.86 AS.seek 0ml
@23:45:40.57 Setting up PCR for ACOPP5 -- writing /var/log/esp/ACOPP521apr2223h.pcr
@23:45:40.59 Building PCR Slug from ASV with MM3 mix, PP5 primer
@23:45:40.61 Priming with carrier
@23:45:40.63 ATV.dial ARV
@23:45:41.29 ARV.dial :carrier
@23:45:41.65 AS.seek 0.5ml
@23:46:07.18 AFV.select :carrierSolution
@23:46:07.30 delay 2 seconds
@23:46:09.33 AS.reconfigure ASconfig
@23:46:09.38 AS.seek 1ml
@23:46:38.41 delay 1 second
@23:46:39.43 AFV.select :holdingCoil
@23:46:39.53 delay 1 second
@23:46:40.55 ATV.dial :waste
@23:46:42.34 AS.reconfigure ASnorm
@23:46:42.40 AS.seek 0ml
@23:47:33.10 AFV.select :carrierSolution
@23:47:33.21 delay 2 seconds
@23:47:35.24 AS.reconfigure ASconfig
@23:47:35.29 AS.seek 0.5ml
@23:48:04.35 delay 1 second
@23:48:05.37 AFV.select :holdingCoil
@23:48:05.47 Building Slug
@23:48:05.48 ATV.dial :ARV
@23:48:07.23 delay 2 seconds
@23:48:09.25 ARV.dial :air1
@23:48:09.65 delay 2 seconds
@23:48:11.72 AS.reconfigure ASslow2
@23:48:11.78 AS.seek 0.506ml
@23:48:18.19 delay 2 seconds
@23:48:20.21 ARV.dial :MM3
@23:48:21.34 delay 2 seconds
@23:48:23.36 Adding MM3
@23:48:23.44 AS.seek 0.524ml
@23:48:41.69 delay 25 seconds
@23:49:06.71 ARV.dial :PP5
@23:49:07.13 Adding PP5
@23:49:07.14 delay 2 seconds
@23:49:09.22 AS.seek 0.53ml
@23:49:15.71 delay 10 seconds
@23:49:25.74 ARV.dial :air3
@23:49:26.15 adding ARV air
@23:49:26.16 delay 2 seconds
@23:49:28.24 AS.seek 0.536ml
@23:49:34.66 delay 5 seconds
@23:49:39.68 ARV.dial :carrier
@23:49:41.01 delay 7 seconds
@23:49:48.08 AS.seek 0.594ml
@23:50:47.15 delay 5 seconds
@23:50:52.17 ATV.dial :ASV
@23:50:52.60 delay 2 seconds
@23:50:54.67 AS.seek 0.6ml
@23:51:01.04 delay 4 seconds
@23:51:05.06 ATV.dial ARV
@23:51:05.65 delay 2 seconds
@23:51:07.67 ARV.dial :carrier
@23:51:07.68 delay 2 seconds
@23:51:09.76 AS.seek 0.65ml
@23:52:00.40 delay 4 seconds
@23:52:04.42 ATV.dial PCR
@23:52:05.07 ASV.dial :waste1
@23:52:05.08 ARV.dial :air1
@23:52:05.51 Positioning Slug by 0.324ml
@23:52:05.60 AS.seek 0.326ml
@23:57:32.26 delay 5 seconds
@23:57:37.28 ATV.dial ARV
@23:57:38.10 ASV.dial :PCRlock
@23:57:38.51 delay 5 seconds
@23:57:43.53 Running ACOPP5 ProfileIPc thermocycling
@23:57:43.54 <PCR> PCR.program ProfileIPc
@23:57:43.66 ProfileIPc should complete in < 1:26:35
@23:57:43.67 PCR.run 1:53:13.75
@01:06:53.57CEST23-Apr-21 PCR.off
@01:06:54.12 <MFBPCR> PCR for ACOPP5 completed -- closed /var/log/esp/ACOPP521apr2223h.pcr
@01:06:54.20 Cleaning PCR
@01:06:54.21 AFV.select :carrierSolution
@01:06:54.32 delay 2 seconds
@01:06:56.35 AS.reconfigure ASconfig
@01:06:56.40 AS.seek 0.5ml
@01:07:06.72 delay 1 second
@01:07:07.74 AFV.select :holdingCoil
@01:07:07.84 ASV.dial :waste1
@01:07:08.24 ATV.dial :air3
@01:07:09.59 AS.reconfigure ASslow1
@01:07:09.65 AS.seek 0.51ml
@01:07:12.08 delay 2 seconds
@01:07:14.10 ATV.dial :bleach
@01:07:14.74 AS.seek 0.81ml
@01:08:15.46 delay 3 seconds
@01:08:18.48 ATV.dial :air3
@01:08:19.07 delay 3 seconds
@01:08:22.14 AS.seek 0.835ml
@01:08:27.44 delay 3 seconds
@01:08:30.46 ATV.dial :bleach
@01:08:31.10 AS.seek 0.82ml
@01:08:34.51 delay 3 seconds
@01:08:37.53 ARV.dial :waste
@01:08:38.58 delay 3 seconds
@01:08:41.60 ATV.dial :ARV
@01:08:42.54 AS.reconfigure ASconfig
@01:08:42.60 AS.seek 0.47ml
@01:09:03.01 AFV.select :carrierSolution
@01:09:03.12 delay 2 seconds
@01:09:05.15 AS.seek 1ml
@01:09:35.94 delay 1 second
@01:09:36.96 AFV.select :holdingCoil
@01:09:37.06 delay 1 second
@01:09:38.09 AS.reconfigure ASfast
@01:09:38.14 AS.seek 0ml
@01:10:05.49 AFV.select :carrierSolution
@01:10:05.60 delay 2 seconds
@01:10:07.63 AS.reconfigure ASconfig
@01:10:07.68 AS.seek 1ml
@01:11:05.43 delay 1 second
@01:11:06.45 AFV.select :holdingCoil
@01:11:06.55 delay 1 second
@01:11:07.58 AS.reconfigure ASfast
@01:11:07.63 AS.seek 0ml
@01:11:34.97 AFV.select :carrierSolution
@01:11:35.08 delay 2 seconds
@01:11:37.10 AS.reconfigure ASconfig
@01:11:37.16 AS.seek 1ml
@01:12:34.91 delay 1 second
@01:12:35.93 AFV.select :holdingCoil
@01:12:36.03 delay 1 second
@01:12:37.06 AS.reconfigure ASfast
@01:12:37.11 AS.seek 0ml
@01:13:04.46 ARV.dial between carrier and air1
@01:13:04.99 ATV.dial :air3
@01:13:06.35 AS.reconfigure ASslow1
@01:13:06.41 AS.seek 0.01ml
@01:13:08.74 delay 2 seconds
@01:13:10.76 ATV.dial :bleach
@01:13:11.42 AS.seek 0.81ml
@01:15:52.71 delay 3 seconds
@01:15:55.73 ATV.dial :air3
@01:15:56.32 delay 3 seconds
@01:15:59.39 AS.seek 0.835ml
@01:16:04.68 delay 3 seconds
@01:16:07.70 ATV.dial :bleach
@01:16:08.35 AS.seek 0.82ml
@01:16:11.66 delay 3 seconds
@01:16:14.68 ATV.dial PCR
@01:16:15.10 AS.reconfigure ASconfig
@01:16:15.16 AS.seek 0ml
@01:17:02.58 PCR.program :bleachProfile
@01:17:02.64 PCR program should complete in < 2:45
@01:17:02.65 PCR.run 10:00
@01:19:58.44 PCR.off
@01:19:58.48 AFV.select :carrierSolution
@01:19:58.59 delay 2 seconds
@01:20:00.62 AS.seek 1ml
@01:20:58.40 delay 1 second
@01:20:59.42 AFV.select :holdingCoil
@01:20:59.52 delay 1 second
@01:21:00.54 AS.reconfigure ASfast
@01:21:00.60 AS.seek 0ml
@01:21:27.96 delay 3 seconds
@01:21:30.98 AFV.select :carrierSolution
@01:21:31.08 delay 2 seconds
@01:21:33.50 AS.reconfigure ASconfig
@01:21:33.58 AS.seek 1ml
@01:22:31.32 delay 1 second
@01:22:32.34 AFV.select :holdingCoil
@01:22:32.44 delay 1 second
@01:22:33.47 AS.reconfigure ASfast
@01:22:33.52 AS.seek 0ml
@01:23:00.86 delay 3 seconds
@01:23:03.88 AFV.select :carrierSolution
@01:23:03.98 delay 2 seconds
@01:23:06.00 AS.reconfigure ASconfig
@01:23:06.07 AS.seek 1ml
@01:24:03.86 delay 1 second
@01:24:04.88 AFV.select :holdingCoil
@01:24:04.98 delay 1 second
@01:24:06.01 AS.reconfigure ASfast
@01:24:06.06 AS.seek 0ml
@01:24:33.40 delay 3 seconds
@01:24:36.42 AFV.select :carrierSolution
@01:24:36.52 delay 2 seconds
@01:24:38.54 AS.reconfigure ASconfig
@01:24:38.60 AS.seek 1ml
@01:25:36.35 delay 1 second
@01:25:37.37 AFV.select :holdingCoil
@01:25:37.47 delay 1 second
@01:25:38.50 AS.reconfigure ASfast
@01:25:38.55 AS.seek 0ml
@01:26:05.90 delay 3 seconds
@01:26:08.92 AFV.select :carrierSolution
@01:26:09.02 delay 2 seconds
@01:26:11.05 AS.reconfigure ASconfig
@01:26:11.11 AS.seek 1ml
@01:27:08.85 delay 1 second
@01:27:09.87 AFV.select :holdingCoil
@01:27:09.97 delay 1 second
@01:27:11.00 AS.reconfigure ASnorm
@01:27:11.05 AS.seek 0ml
@01:28:01.77 Setting up PCR for AIPc -- writing /var/log/esp/AIPc21apr2301h.pcr
@01:28:01.79 Building PCR Slug from ASV with MM3 mix, PP6 primer
@01:28:01.81 Priming with carrier
@01:28:01.83 ATV.dial ARV
@01:28:02.49 ARV.dial :carrier
@01:28:02.85 AS.seek 0.5ml
@01:28:28.44 AFV.select :carrierSolution
@01:28:28.56 delay 2 seconds
@01:28:30.59 AS.reconfigure ASconfig
@01:28:30.64 AS.seek 1ml
@01:28:59.66 delay 1 second
@01:29:00.68 AFV.select :holdingCoil
@01:29:00.78 delay 1 second
@01:29:01.80 ATV.dial :waste
@01:29:03.58 AS.reconfigure ASnorm
@01:29:03.65 AS.seek 0ml
@01:29:54.33 AFV.select :carrierSolution
@01:29:54.44 delay 2 seconds
@01:29:56.47 AS.reconfigure ASconfig
@01:29:56.52 AS.seek 0.5ml
@01:30:25.55 delay 1 second
@01:30:26.57 AFV.select :holdingCoil
@01:30:26.67 Building Slug
@01:30:26.68 ATV.dial :ARV
@01:30:28.43 delay 2 seconds
@01:30:30.45 ARV.dial :air1
@01:30:30.86 delay 2 seconds
@01:30:32.93 AS.reconfigure ASslow2
@01:30:32.99 AS.seek 0.506ml
@01:30:39.66 delay 2 seconds
@01:30:41.68 ARV.dial :MM3
@01:30:42.79 delay 2 seconds
@01:30:44.81 Adding MM3
@01:30:44.90 AS.seek 0.524ml
@01:31:03.21 delay 25 seconds
@01:31:28.23 ARV.dial :PP6
@01:31:28.63 Adding PP6
@01:31:28.64 delay 2 seconds
@01:31:30.72 AS.seek 0.53ml
@01:31:37.22 delay 10 seconds
@01:31:47.25 ARV.dial :air1
@01:31:48.11 adding ARV air
@01:31:48.12 delay 2 seconds
@01:31:50.20 AS.seek 0.536ml
@01:31:56.93 delay 5 seconds
@01:32:01.95 ARV.dial :carrier
@01:32:02.36 delay 7 seconds
@01:32:09.44 AS.seek 0.594ml
@01:33:08.27 delay 5 seconds
@01:33:13.29 ATV.dial :ASV
@01:33:13.72 delay 2 seconds
@01:33:15.79 AS.seek 0.6ml
@01:33:22.35 delay 4 seconds
@01:33:26.37 ATV.dial ARV
@01:33:26.96 delay 2 seconds
@01:33:28.98 ARV.dial :carrier
@01:33:28.99 delay 2 seconds
@01:33:31.07 AS.seek 0.65ml
@01:34:21.61 delay 4 seconds
@01:34:25.63 ATV.dial PCR
@01:34:26.29 ASV.dial :waste1
@01:34:26.30 ARV.dial :air1
@01:34:26.72 Positioning Slug by 0.324ml
@01:34:26.81 AS.seek 0.326ml
@01:39:53.16 delay 5 seconds
@01:39:58.18 ATV.dial ARV
@01:39:59.00 ASV.dial :PCRlock
@01:39:59.41 delay 5 seconds
@01:40:04.43 Running AIPc ProfileIPc thermocycling
@01:40:04.44 <PCR> PCR.program ProfileIPc
@01:40:04.56 ProfileIPc should complete in < 1:26:35
@01:40:04.57 PCR.run 1:53:13.75
@02:49:07.53 PCR.off
@02:49:08.08 <MFBPCR> PCR for AIPc completed -- closed /var/log/esp/AIPc21apr2301h.pcr
@02:49:08.10 Full MFB Decontamination
@02:49:08.12 Decon extraction column and decon extSrc
@02:49:08.14 SPE.hold 2:46:40,55C
@02:49:08.19 ASV.dial :SPEdelivery
@02:49:08.88 delay 20 seconds
@02:49:28.91 AFV.select :carrierSolution
@02:49:29.01 delay 2 seconds
@02:49:31.04 AS.reconfigure ASconfig
@02:49:31.09 AS.seek 0.5ml
@02:49:41.80 delay 1 second
@02:49:42.82 AFV.select :holdingCoil
@02:49:42.92 ATV.dial :air1
@02:49:44.99 AS.reconfigure ASslow1
@02:49:45.05 AS.seek 0.52ml
@02:49:49.39 ATV.dial :ASV
@02:49:51.33 ASV.dial :SPErecovery
@02:49:52.03 AS.seek 0.62ml
@02:50:12.41 ATV.dial :waste
@02:50:14.63 AS.reconfigure ASnorm
@02:50:14.69 AS.seek 0ml
@02:50:46.28 deconEluateRecoveryLine extSrc
@02:50:46.29 ATV.dial :waste
@02:50:46.32 AS.seek 0ml
@02:50:46.40 ATV.dial :air3
@02:50:47.19 AS.reconfigure ASslow1
@02:50:47.25 AS.seek 0.01ml
@02:50:49.53 delay 2 seconds
@02:50:51.55 ATV.dial :bleach
@02:50:52.06 AS.seek 0.31ml
@02:51:52.71 delay 3 seconds
@02:51:55.73 ATV.dial :air3
@02:51:56.36 delay 3 seconds
@02:51:59.43 AS.seek 0.335ml
@02:52:04.64 delay 3 seconds
@02:52:07.66 ATV.dial :bleach
@02:52:08.34 AS.seek 0.32ml
@02:52:11.63 delay 3 seconds
@02:52:14.65 ATV.dial :extSrc
@02:52:15.33 AS.seek 0ml
@02:53:19.96 AFV.select :carrierSolution
@02:53:20.07 delay 2 seconds
@02:53:22.10 AS.reconfigure ASconfig
@02:53:22.15 AS.seek 0.5ml
@02:53:51.19 delay 1 second
@02:53:52.21 AFV.select :holdingCoil
@02:53:52.31 ATV.dial :extSrc
@02:53:52.33 AS.reconfigure ASslow1
@02:53:52.40 AS.seek 0ml
@02:55:33.22 AFV.select :carrierSolution
@02:55:33.33 delay 2 seconds
@02:55:35.36 AS.reconfigure ASconfig
@02:55:35.41 AS.seek 0.5ml
@02:56:04.46 delay 1 second
@02:56:05.48 AFV.select :holdingCoil
@02:56:05.58 ATV.dial :extSrc
@02:56:05.60 AS.reconfigure ASslow1
@02:56:05.67 AS.seek 0ml
@02:57:46.52 AFV.select :carrierSolution
@02:57:46.63 delay 2 seconds
@02:57:48.66 AS.reconfigure ASconfig
@02:57:48.71 AS.seek 0.5ml
@02:58:17.75 delay 1 second
@02:58:18.77 AFV.select :holdingCoil
@02:58:18.87 ATV.dial :extSrc
@02:58:18.89 AS.reconfigure ASslow1
@02:58:18.96 AS.seek 0ml
@02:59:59.91 AFV.select :carrierSolution
@03:00:00.02 delay 2 seconds
@03:00:02.05 AS.reconfigure ASconfig
@03:00:02.10 AS.seek 0.5ml
@03:00:31.10 delay 1 second
@03:00:32.12 AFV.select :holdingCoil
@03:00:32.22 ATV.dial :extSrc
@03:00:32.24 AS.reconfigure ASslow1
@03:00:32.30 AS.seek 0ml
@03:02:13.14 ATV.dial :air2
@03:02:14.53 AS.reconfigure ASslow0
@03:02:14.58 AS.seek 0.5ml
@03:03:05.18 ATV.dial :extSrc
@03:03:06.77 AS.seek 0.02ml
@03:03:55.39 delay 1 second
@03:03:56.41 ATV.dial :waste
@03:03:57.75 AS.reconfigure ASconfig
@03:03:57.81 AS.seek 0ml
@03:03:59.25 AFV.select :holdingCoil
@03:03:59.26 ATV.dial :waste
@03:03:59.29 AS.seek 0ml
@03:03:59.41 AFV.select :carrierSolution
@03:03:59.52 delay 2 seconds
@03:04:01.55 AS.seek 0.5ml
@03:04:30.63 delay 1 second
@03:04:31.65 AFV.select :holdingCoil
@03:04:31.75 ATV.dial :air3
@03:04:32.63 AS.reconfigure ASslow1
@03:04:32.69 AS.seek 0.52ml
@03:04:36.91 ATV.dial :air3
@03:04:37.00 AS.seek 0.53ml
@03:04:39.39 delay 2 seconds
@03:04:41.41 ATV.dial :bleach
@03:04:41.88 AS.seek 0.71ml
@03:05:18.35 delay 3 seconds
@03:05:21.37 ATV.dial :air3
@03:05:21.96 delay 3 seconds
@03:05:25.03 AS.seek 0.735ml
@03:05:30.36 delay 3 seconds
@03:05:33.38 ATV.dial :bleach
@03:05:34.03 AS.seek 0.72ml
@03:05:37.33 delay 3 seconds
@03:05:40.35 ATV.dial ASV
@03:05:41.46 ASV.dial :SPErecovery
@03:05:41.54 AS.seek 0.365ml
@03:06:53.22 ATV.dial :waste
@03:06:55.02 AS.reconfigure ASnorm
@03:06:55.08 AS.seek 0ml
@03:07:13.83 ATV.dial :air3
@03:07:14.98 AS.reconfigure ASslow1
@03:07:15.05 AS.seek 0.01ml
@03:07:17.33 delay 2 seconds
@03:07:19.35 ATV.dial :bleach
@03:07:19.83 AS.seek 0.58ml
@03:09:14.72 delay 3 seconds
@03:09:17.74 ATV.dial :air3
@03:09:18.35 delay 3 seconds
@03:09:21.45 AS.seek 0.605ml
@03:09:26.88 delay 3 seconds
@03:09:29.90 ATV.dial :bleach
@03:09:30.64 AS.seek 0.59ml
@03:09:33.91 delay 3 seconds
@03:09:36.93 ATV.dial :ASV
@03:09:38.02 ASV.dial :wasteR
@03:09:38.44 AS.seek 0ml
@03:11:37.41 delay 1:00
@03:12:37.43 ATV.dial ASV
@03:12:37.44 AFV.select :carrierSolution
@03:12:37.56 delay 2 seconds
@03:12:39.58 AS.reconfigure ASconfig
@03:12:39.64 AS.seek 1ml
@03:13:37.39 delay 1 second
@03:13:38.41 AFV.select :holdingCoil
@03:13:38.51 delay 1 second
@03:13:39.53 ASV.dial :wasteR
@03:13:39.55 AS.reconfigure ASslow1
@03:13:39.62 AS.seek 0ml
@03:17:01.03 delay 10 seconds
@03:17:11.06 ATV.dial :air3
@03:17:12.67 AS.seek 0.01ml
@03:17:15.11 delay 2 seconds
@03:17:17.13 ATV.dial :bleach
@03:17:17.78 AS.seek 0.58ml
@03:19:12.71 delay 3 seconds
@03:19:15.73 ATV.dial :air3
@03:19:16.33 delay 3 seconds
@03:19:19.40 AS.seek 0.605ml
@03:19:24.83 delay 3 seconds
@03:19:27.85 ATV.dial :bleach
@03:19:28.50 AS.seek 0.59ml
@03:19:31.78 delay 3 seconds
@03:19:34.80 ATV.dial ASV
@03:19:35.91 ASV.dial :SPEdelivery
@03:19:36.30 AS.seek 0ml
@03:21:35.22 delay 1:00
@03:22:35.24 AFV.select :carrierSolution
@03:22:35.35 delay 2 seconds
@03:22:37.38 AS.reconfigure ASconfig
@03:22:37.43 AS.seek 1ml
@03:23:35.19 delay 1 second
@03:23:36.21 AFV.select :holdingCoil
@03:23:36.31 delay 1 second
@03:23:37.33 ATV.dial ASV
@03:23:37.34 ASV.dial :SPEdelivery
@03:23:37.37 AS.reconfigure ASslow1
@03:23:37.43 AS.seek 0ml
@03:26:58.83 delay 10 seconds
@03:27:08.86 ATV.dial :air2
@03:27:09.74 AS.reconfigure ASslow0
@03:27:09.80 AS.seek 0.8ml
@03:28:30.61 delay 3 seconds
@03:28:33.63 ATV.dial :ASV
@03:28:34.67 ASV.dial :SPEdelivery
@03:28:34.69 AS.reconfigure ASslow1
@03:28:34.76 AS.seek 0.03ml
@03:31:09.83 delay 20 seconds
@03:31:29.86 ATV.dial :waste,via: :air3
@03:31:31.69 AS.reconfigure ASconfig
@03:31:31.75 AS.seek 0ml
@03:31:33.78 SPE.coast
@03:31:33.86 Removing Bleach from Mixing Coil
@03:31:33.87 AFV.select :carrierSolution
@03:31:33.98 delay 2 seconds
@03:31:36.01 AS.seek 0.5ml
@03:32:05.02 delay 1 second
@03:32:06.04 AFV.select :holdingCoil
@03:32:06.14 ATV.dial :ASV
@03:32:07.94 ASV.dial :SPErecovery
@03:32:08.58 AS.reconfigure ASslow1
@03:32:08.64 AS.seek 0.73ml
@03:32:55.24 ASV.dial :wasteD
@03:32:55.85 AS.reconfigure ASnorm
@03:32:55.91 AS.seek 0ml
@03:33:32.99 AFV.select :carrierSolution
@03:33:33.10 delay 2 seconds
@03:33:35.13 AS.reconfigure ASconfig
@03:33:35.18 AS.seek 0.5ml
@03:34:04.21 delay 1 second
@03:34:05.23 AFV.select :holdingCoil
@03:34:05.33 ATV.dial ASV
@03:34:05.35 ASV.dial :SPErecovery
@03:34:06.01 AS.reconfigure ASslow0
@03:34:06.08 AS.seek 0.3ml
@03:34:26.52 ATV.dial :air2
@03:34:27.61 AS.reconfigure ASslow1
@03:34:27.67 AS.seek 0.32ml
@03:34:32.12 ATV.dial ASV
@03:34:33.21 AS.reconfigure ASslow0
@03:34:33.27 AS.seek 0.57ml
@03:34:58.77 ASV.dial :wasteD
@03:34:59.38 AS.reconfigure ASnorm
@03:34:59.44 AS.seek 0ml
@03:35:28.50 AFV.select :carrierSolution
@03:35:28.60 delay 2 seconds
@03:35:30.63 AS.reconfigure ASconfig
@03:35:30.68 AS.seek 0.5ml
@03:35:59.75 delay 1 second
@03:36:00.77 AFV.select :holdingCoil
@03:36:00.87 ATV.dial ASV
@03:36:00.89 ASV.dial :SPErecovery
@03:36:01.99 AS.reconfigure ASslow0
@03:36:02.05 AS.seek 0.3ml
@03:36:22.50 ATV.dial :air2
@03:36:23.58 AS.reconfigure ASslow1
@03:36:23.64 AS.seek 0.32ml
@03:36:28.07 ATV.dial ASV
@03:36:29.16 AS.reconfigure ASslow0
@03:36:29.22 AS.seek 0.57ml
@03:36:54.72 ASV.dial :wasteD
@03:36:55.33 AS.reconfigure ASnorm
@03:36:55.40 AS.seek 0ml
@03:37:24.47 ATV.dial :air2
@03:37:25.50 AS.reconfigure ASslow0
@03:37:25.56 AS.seek 0.3ml
@03:37:56.08 ATV.dial :ASV
@03:37:57.11 ASV.dial :wasteD
@03:37:57.13 AS.reconfigure ASconfig
@03:37:57.19 AS.seek 0.05ml
@03:38:11.88 ATV.dial :waste
@03:38:13.85 AS.seek 0ml
@03:38:17.03 ASV.dial :SPEdelivery
@03:38:18.10 Cleaning PCR
@03:38:18.11 AFV.select :carrierSolution
@03:38:18.23 delay 2 seconds
@03:38:20.26 AS.seek 0.5ml
@03:38:49.27 delay 1 second
@03:38:50.29 AFV.select :holdingCoil
@03:38:50.39 ASV.dial :waste1
@03:38:50.78 ATV.dial :air3
@03:38:51.47 AS.reconfigure ASslow1
@03:38:51.53 AS.seek 0.51ml
@03:38:53.91 delay 2 seconds
@03:38:55.93 ATV.dial :bleach
@03:38:56.41 AS.seek 0.81ml
@03:39:57.03 delay 3 seconds
@03:40:00.05 ATV.dial :air3
@03:40:00.66 delay 3 seconds
@03:40:03.73 AS.seek 0.835ml
@03:40:09.20 delay 3 seconds
@03:40:12.22 ATV.dial :bleach
@03:40:12.87 AS.seek 0.82ml
@03:40:16.16 delay 3 seconds
@03:40:19.18 ARV.dial :waste
@03:40:19.81 delay 3 seconds
@03:40:22.83 ATV.dial :ARV
@03:40:23.76 AS.reconfigure ASconfig
@03:40:23.82 AS.seek 0.47ml
@03:40:44.24 AFV.select :carrierSolution
@03:40:44.35 delay 2 seconds
@03:40:46.38 AS.seek 1ml
@03:41:17.17 delay 1 second
@03:41:18.19 AFV.select :holdingCoil
@03:41:18.29 delay 1 second
@03:41:19.31 AS.reconfigure ASfast
@03:41:19.37 AS.seek 0ml
@03:41:46.72 AFV.select :carrierSolution
@03:41:46.83 delay 2 seconds
@03:41:48.85 AS.reconfigure ASconfig
@03:41:48.91 AS.seek 1ml
@03:42:46.69 delay 1 second
@03:42:47.71 AFV.select :holdingCoil
@03:42:47.81 delay 1 second
@03:42:48.83 AS.reconfigure ASfast
@03:42:48.89 AS.seek 0ml
@03:43:16.24 AFV.select :carrierSolution
@03:43:16.35 delay 2 seconds
@03:43:18.37 AS.reconfigure ASconfig
@03:43:18.43 AS.seek 1ml
@03:44:16.22 delay 1 second
@03:44:17.24 AFV.select :holdingCoil
@03:44:17.34 delay 1 second
@03:44:18.36 AS.reconfigure ASfast
@03:44:18.42 AS.seek 0ml
@03:44:45.75 ARV.dial between carrier and air1
@03:44:46.28 ATV.dial :air3
@03:44:47.65 AS.reconfigure ASslow1
@03:44:47.71 AS.seek 0.01ml
@03:44:50.06 delay 2 seconds
@03:44:52.08 ATV.dial :bleach
@03:44:52.75 AS.seek 0.81ml
@03:47:34.05 delay 3 seconds
@03:47:37.07 ATV.dial :air3
@03:47:37.67 delay 3 seconds
@03:47:40.74 AS.seek 0.835ml
@03:47:46.06 delay 3 seconds
@03:47:49.08 ATV.dial :bleach
@03:47:49.73 AS.seek 0.82ml
@03:47:53.02 delay 3 seconds
@03:47:56.04 ATV.dial PCR
@03:47:56.46 AS.reconfigure ASconfig
@03:47:56.52 AS.seek 0ml
@03:48:43.94 PCR.program :bleachProfile
@03:48:44.01 PCR program should complete in < 2:45
@03:48:44.02 PCR.run 10:00
@03:51:46.30 PCR.off
@03:51:46.34 AFV.select :carrierSolution
@03:51:46.45 delay 2 seconds
@03:51:48.48 AS.seek 1ml
@03:52:46.25 delay 1 second
@03:52:47.27 AFV.select :holdingCoil
@03:52:47.37 delay 1 second
@03:52:48.39 AS.reconfigure ASfast
@03:52:48.45 AS.seek 0ml
@03:53:15.80 delay 3 seconds
@03:53:18.82 AFV.select :carrierSolution
@03:53:18.92 delay 2 seconds
@03:53:20.95 AS.reconfigure ASconfig
@03:53:21.01 AS.seek 1ml
@03:54:18.80 delay 1 second
@03:54:19.82 AFV.select :holdingCoil
@03:54:19.92 delay 1 second
@03:54:20.95 AS.reconfigure ASfast
@03:54:21.00 AS.seek 0ml
@03:54:48.35 delay 3 seconds
@03:54:51.37 AFV.select :carrierSolution
@03:54:51.47 delay 2 seconds
@03:54:53.49 AS.reconfigure ASconfig
@03:54:53.55 AS.seek 1ml
@03:55:51.31 delay 1 second
@03:55:52.33 AFV.select :holdingCoil
@03:55:52.43 delay 1 second
@03:55:53.45 AS.reconfigure ASfast
@03:55:53.51 AS.seek 0ml
@03:56:20.85 delay 3 seconds
@03:56:23.87 AFV.select :carrierSolution
@03:56:23.97 delay 2 seconds
@03:56:25.99 AS.reconfigure ASconfig
@03:56:26.06 AS.seek 1ml
@03:57:23.81 delay 1 second
@03:57:24.83 AFV.select :holdingCoil
@03:57:24.93 delay 1 second
@03:57:25.95 AS.reconfigure ASfast
@03:57:26.01 AS.seek 0ml
@03:57:53.36 delay 3 seconds
@03:57:56.38 AFV.select :carrierSolution
@03:57:56.48 delay 2 seconds
@03:57:58.51 AS.reconfigure ASconfig
@03:57:58.56 AS.seek 1ml
@03:58:56.39 delay 1 second
@03:58:57.41 AFV.select :holdingCoil
@03:58:57.51 delay 1 second
@03:58:58.53 AS.reconfigure ASnorm
@03:58:58.59 AS.seek 0ml
@03:59:49.30 AFV.select :carrierSolution
@03:59:49.40 delay 2 seconds
@03:59:51.43 AS.reconfigure ASconfig
@03:59:51.48 AS.seek 1ml
@04:00:49.22 delay 1 second
@04:00:50.24 AFV.select :holdingCoil
@04:00:50.34 delay 1 second
@04:00:51.36 ATV.dial :waste
@04:00:52.67 AS.reconfigure ASnorm
@04:00:52.73 AS.seek 0.5ml
@04:01:18.30 ATV.dial :waste2
@04:01:19.64 AS.seek 0ml
@04:01:45.19 <MAIN> Cleaning PCR
@04:01:45.20 AFV.select :carrierSolution
@04:01:45.31 delay 2 seconds
@04:01:47.34 AS.reconfigure ASconfig
@04:01:47.39 AS.seek 0.5ml
@04:02:16.41 delay 1 second
@04:02:17.43 AFV.select :holdingCoil
@04:02:17.53 ASV.dial :waste1
@04:02:17.54 ATV.dial :air3
@04:02:19.58 AS.reconfigure ASslow1
@04:02:19.64 AS.seek 0.51ml
@04:02:21.92 delay 2 seconds
@04:02:23.94 ATV.dial :bleach
@04:02:24.42 AS.seek 0.81ml
@04:03:25.00 delay 3 seconds
@04:03:28.02 ATV.dial :air3
@04:03:28.61 delay 3 seconds
@04:03:31.68 AS.seek 0.835ml
@04:03:36.96 delay 3 seconds
@04:03:39.98 ATV.dial :bleach
@04:03:40.62 AS.seek 0.82ml
@04:03:44.30 delay 3 seconds
@04:03:47.32 ARV.dial :waste
@04:03:47.85 delay 3 seconds
@04:03:50.87 ATV.dial :ARV
@04:03:51.77 AS.reconfigure ASconfig
@04:03:51.83 AS.seek 0.47ml
@04:04:12.24 AFV.select :carrierSolution
@04:04:12.35 delay 2 seconds
@04:04:14.38 AS.seek 1ml
@04:04:45.13 delay 1 second
@04:04:46.15 AFV.select :holdingCoil
@04:04:46.25 delay 1 second
@04:04:47.28 AS.reconfigure ASfast
@04:04:47.33 AS.seek 0ml
@04:05:14.67 AFV.select :carrierSolution
@04:05:14.78 delay 2 seconds
@04:05:16.81 AS.reconfigure ASconfig
@04:05:16.86 AS.seek 1ml
@04:06:14.64 delay 1 second
@04:06:15.66 AFV.select :holdingCoil
@04:06:15.76 delay 1 second
@04:06:16.79 AS.reconfigure ASfast
@04:06:16.84 AS.seek 0ml
@04:06:44.17 AFV.select :carrierSolution
@04:06:44.28 delay 2 seconds
@04:06:46.30 AS.reconfigure ASconfig
@04:06:46.36 AS.seek 1ml
@04:07:44.10 delay 1 second
@04:07:45.12 AFV.select :holdingCoil
@04:07:45.22 delay 1 second
@04:07:46.25 AS.reconfigure ASfast
@04:07:46.30 AS.seek 0ml
@04:08:13.64 ARV.dial between carrier and air1
@04:08:14.16 ATV.dial :air3
@04:08:15.50 AS.reconfigure ASslow1
@04:08:15.56 AS.seek 0.01ml
@04:08:17.95 delay 2 seconds
@04:08:19.97 ATV.dial :bleach
@04:08:20.62 AS.seek 0.81ml
@04:11:01.78 delay 3 seconds
@04:11:04.80 ATV.dial :air3
@04:11:05.39 delay 3 seconds
@04:11:08.46 AS.seek 0.835ml
@04:11:13.78 delay 3 seconds
@04:11:16.80 ATV.dial :bleach
@04:11:17.45 AS.seek 0.82ml
@04:11:20.67 delay 3 seconds
@04:11:23.69 ATV.dial PCR
@04:11:24.11 AS.reconfigure ASconfig
@04:11:24.17 AS.seek 0ml
@04:12:11.58 PCR.program :bleachProfile
@04:12:11.64 PCR program should complete in < 2:45
@04:12:11.66 PCR.run 10:00
@04:15:10.49 PCR.off
@04:15:10.53 AFV.select :carrierSolution
@04:15:10.64 delay 2 seconds
@04:15:12.67 AS.seek 1ml
@04:16:10.44 delay 1 second
@04:16:11.46 AFV.select :holdingCoil
@04:16:11.56 delay 1 second
@04:16:12.59 AS.reconfigure ASfast
@04:16:12.64 AS.seek 0ml
@04:16:39.99 delay 3 seconds
@04:16:43.01 AFV.select :carrierSolution
@04:16:43.11 delay 2 seconds
@04:16:45.44 AS.reconfigure ASconfig
@04:16:45.55 AS.seek 1ml
@04:17:43.35 delay 1 second
@04:17:44.37 AFV.select :holdingCoil
@04:17:44.47 delay 1 second
@04:17:45.50 AS.reconfigure ASfast
@04:17:45.55 AS.seek 0ml
@04:18:12.89 delay 3 seconds
@04:18:15.91 AFV.select :carrierSolution
@04:18:16.01 delay 2 seconds
@04:18:18.03 AS.reconfigure ASconfig
@04:18:18.10 AS.seek 1ml
@04:19:15.85 delay 1 second
@04:19:16.87 AFV.select :holdingCoil
@04:19:16.97 delay 1 second
@04:19:18.00 AS.reconfigure ASfast
@04:19:18.05 AS.seek 0ml
@04:19:45.39 delay 3 seconds
@04:19:48.41 AFV.select :carrierSolution
@04:19:48.51 delay 2 seconds
@04:19:50.54 AS.reconfigure ASconfig
@04:19:50.59 AS.seek 1ml
@04:20:48.42 delay 1 second
@04:20:49.44 AFV.select :holdingCoil
@04:20:49.54 delay 1 second
@04:20:50.57 AS.reconfigure ASfast
@04:20:50.62 AS.seek 0ml
@04:21:17.97 delay 3 seconds
@04:21:20.99 AFV.select :carrierSolution
@04:21:21.09 delay 2 seconds
@04:21:23.11 AS.reconfigure ASconfig
@04:21:23.18 AS.seek 1ml
@04:22:20.92 delay 1 second
@04:22:21.94 AFV.select :holdingCoil
@04:22:22.04 delay 1 second
@04:22:23.07 AS.reconfigure ASnorm
@04:22:23.12 AS.seek 0ml
@04:23:13.85 delay 5 seconds
@04:23:18.92 Cleaning PCR
@04:23:18.93 AFV.select :carrierSolution
@04:23:19.05 delay 2 seconds
@04:23:21.08 AS.reconfigure ASconfig
@04:23:21.13 AS.seek 0.5ml
@04:23:50.17 delay 1 second
@04:23:51.19 AFV.select :holdingCoil
@04:23:51.29 ASV.dial :waste1
@04:23:51.30 ATV.dial :air3
@04:23:52.21 AS.reconfigure ASslow1
@04:23:52.27 AS.seek 0.51ml
@04:23:54.56 delay 2 seconds
@04:23:56.58 ATV.dial :bleach
@04:23:57.23 AS.seek 0.81ml
@04:24:57.83 delay 3 seconds
@04:25:00.85 ATV.dial :air3
@04:25:01.45 delay 3 seconds
@04:25:04.52 AS.seek 0.835ml
@04:25:09.92 delay 3 seconds
@04:25:12.94 ATV.dial :bleach
@04:25:13.59 AS.seek 0.82ml
@04:25:17.00 delay 3 seconds
@04:25:20.02 ARV.dial :waste
@04:25:20.55 delay 3 seconds
@04:25:23.57 ATV.dial :ARV
@04:25:24.49 AS.reconfigure ASconfig
@04:25:24.55 AS.seek 0.47ml
@04:25:44.97 AFV.select :carrierSolution
@04:25:45.08 delay 2 seconds
@04:25:47.11 AS.seek 1ml
@04:26:17.86 delay 1 second
@04:26:18.88 AFV.select :holdingCoil
@04:26:18.98 delay 1 second
@04:26:20.01 AS.reconfigure ASfast
@04:26:20.06 AS.seek 0ml
@04:26:47.39 AFV.select :carrierSolution
@04:26:47.50 delay 2 seconds
@04:26:49.52 AS.reconfigure ASconfig
@04:26:49.58 AS.seek 1ml
@04:27:47.31 delay 1 second
@04:27:48.33 AFV.select :holdingCoil
@04:27:48.43 delay 1 second
@04:27:49.46 AS.reconfigure ASfast
@04:27:49.51 AS.seek 0ml
@04:28:16.85 AFV.select :carrierSolution
@04:28:16.96 delay 2 seconds
@04:28:18.98 AS.reconfigure ASconfig
@04:28:19.04 AS.seek 1ml
@04:29:16.78 delay 1 second
@04:29:17.80 AFV.select :holdingCoil
@04:29:17.90 delay 1 second
@04:29:18.93 AS.reconfigure ASfast
@04:29:18.98 AS.seek 0ml
@04:29:46.31 ARV.dial between carrier and air1
@04:29:46.81 ATV.dial :air3
@04:29:48.16 AS.reconfigure ASslow1
@04:29:48.22 AS.seek 0.01ml
@04:29:50.52 delay 2 seconds
@04:29:52.54 ATV.dial :bleach
@04:29:53.20 AS.seek 0.81ml
@04:32:34.34 delay 3 seconds
@04:32:37.36 ATV.dial :air3
@04:32:37.97 delay 3 seconds
@04:32:41.04 AS.seek 0.835ml
@04:32:46.35 delay 3 seconds
@04:32:49.37 ATV.dial :bleach
@04:32:50.01 AS.seek 0.82ml
@04:32:53.31 delay 3 seconds
@04:32:56.33 ATV.dial PCR
@04:32:56.75 AS.reconfigure ASconfig
@04:32:56.81 AS.seek 0ml
@04:33:44.22 PCR.program :bleachProfile
@04:33:44.28 PCR program should complete in < 2:45
@04:33:44.29 PCR.run 10:00
@04:36:43.49 PCR.off
@04:36:43.53 AFV.select :carrierSolution
@04:36:43.64 delay 2 seconds
@04:36:45.67 AS.seek 1ml
@04:37:43.44 delay 1 second
@04:37:44.46 AFV.select :holdingCoil
@04:37:44.56 delay 1 second
@04:37:45.59 AS.reconfigure ASfast
@04:37:45.64 AS.seek 0ml
@04:38:12.97 delay 3 seconds
@04:38:15.99 AFV.select :carrierSolution
@04:38:16.09 delay 2 seconds
@04:38:18.11 AS.reconfigure ASconfig
@04:38:18.18 AS.seek 1ml
@04:39:15.92 delay 1 second
@04:39:16.94 AFV.select :holdingCoil
@04:39:17.04 delay 1 second
@04:39:18.07 AS.reconfigure ASfast
@04:39:18.12 AS.seek 0ml
@04:39:45.45 delay 3 seconds
@04:39:48.47 AFV.select :carrierSolution
@04:39:48.57 delay 2 seconds
@04:39:50.59 AS.reconfigure ASconfig
@04:39:50.65 AS.seek 1ml
@04:40:48.41 delay 1 second
@04:40:49.43 AFV.select :holdingCoil
@04:40:49.53 delay 1 second
@04:40:50.56 AS.reconfigure ASfast
@04:40:50.61 AS.seek 0ml
@04:41:17.95 delay 3 seconds
@04:41:20.97 AFV.select :carrierSolution
@04:41:21.07 delay 2 seconds
@04:41:23.09 AS.reconfigure ASconfig
@04:41:23.16 AS.seek 1ml
@04:42:20.91 delay 1 second
@04:42:21.93 AFV.select :holdingCoil
@04:42:22.03 delay 1 second
@04:42:23.06 AS.reconfigure ASfast
@04:42:23.11 AS.seek 0ml
@04:42:50.45 delay 3 seconds
@04:42:53.47 AFV.select :carrierSolution
@04:42:53.57 delay 2 seconds
@04:42:55.59 AS.reconfigure ASconfig
@04:42:55.65 AS.seek 1ml
@04:43:53.44 delay 1 second
@04:43:54.46 AFV.select :holdingCoil
@04:43:54.56 delay 1 second
@04:43:55.58 AS.reconfigure ASnorm
@04:43:55.64 AS.seek 0ml
@04:44:46.36 delay 5 seconds
@04:44:51.43 Cleaning PCR
@04:44:51.44 AFV.select :carrierSolution
@04:44:51.56 delay 2 seconds
@04:44:53.59 AS.reconfigure ASconfig
@04:44:53.64 AS.seek 0.5ml
@04:45:22.67 delay 1 second
@04:45:23.69 AFV.select :holdingCoil
@04:45:23.79 ASV.dial :waste1
@04:45:23.80 ATV.dial :air3
@04:45:24.69 AS.reconfigure ASslow1
@04:45:24.75 AS.seek 0.51ml
@04:45:27.11 delay 2 seconds
@04:45:29.13 ATV.dial :bleach
@04:45:29.78 AS.seek 0.81ml
@04:46:30.38 delay 3 seconds
@04:46:33.40 ATV.dial :air3
@04:46:34.00 delay 3 seconds
@04:46:37.07 AS.seek 0.835ml
@04:46:42.46 delay 3 seconds
@04:46:45.48 ATV.dial :bleach
@04:46:46.11 AS.seek 0.82ml
@04:46:49.41 delay 3 seconds
@04:46:52.43 ARV.dial :waste
@04:46:52.94 delay 3 seconds
@04:46:55.96 ATV.dial :ARV
@04:46:56.88 AS.reconfigure ASconfig
@04:46:56.94 AS.seek 0.47ml
@04:47:17.36 AFV.select :carrierSolution
@04:47:17.47 delay 2 seconds
@04:47:19.50 AS.seek 1ml
@04:47:50.28 delay 1 second
@04:47:51.30 AFV.select :holdingCoil
@04:47:51.40 delay 1 second
@04:47:52.42 AS.reconfigure ASfast
@04:47:52.48 AS.seek 0ml
@04:48:19.80 AFV.select :carrierSolution
@04:48:19.91 delay 2 seconds
@04:48:21.93 AS.reconfigure ASconfig
@04:48:21.99 AS.seek 1ml
@04:49:19.74 delay 1 second
@04:49:20.76 AFV.select :holdingCoil
@04:49:20.86 delay 1 second
@04:49:21.89 AS.reconfigure ASfast
@04:49:21.94 AS.seek 0ml
@04:49:49.25 AFV.select :carrierSolution
@04:49:49.36 delay 2 seconds
@04:49:51.38 AS.reconfigure ASconfig
@04:49:51.44 AS.seek 1ml
@04:50:49.53 delay 1 second
@04:50:50.61 AFV.select :holdingCoil
@04:50:50.71 delay 1 second
@04:50:51.74 AS.reconfigure ASfast
@04:50:51.79 AS.seek 0ml
@04:51:19.13 ARV.dial between carrier and air1
@04:51:19.66 ATV.dial :air3
@04:51:21.01 AS.reconfigure ASslow1
@04:51:21.06 AS.seek 0.01ml
@04:51:23.34 delay 2 seconds
@04:51:25.36 ATV.dial :bleach
@04:51:26.01 AS.seek 0.81ml
@04:54:07.28 delay 3 seconds
@04:54:10.30 ATV.dial :air3
@04:54:10.89 delay 3 seconds
@04:54:13.96 AS.seek 0.835ml
@04:54:19.28 delay 3 seconds
@04:54:22.30 ATV.dial :bleach
@04:54:22.95 AS.seek 0.82ml
@04:54:26.36 delay 3 seconds
@04:54:29.38 ATV.dial PCR
@04:54:29.80 AS.reconfigure ASconfig
@04:54:29.86 AS.seek 0ml
@04:55:17.28 PCR.program :bleachProfile
@04:55:17.35 PCR program should complete in < 2:45
@04:55:17.36 PCR.run 10:00
@04:58:16.23 PCR.off
@04:58:16.26 AFV.select :carrierSolution
@04:58:16.38 delay 2 seconds
@04:58:18.41 AS.seek 1ml
@04:59:16.16 delay 1 second
@04:59:17.18 AFV.select :holdingCoil
@04:59:17.28 delay 1 second
@04:59:18.31 AS.reconfigure ASfast
@04:59:18.36 AS.seek 0ml
@04:59:45.70 delay 3 seconds
@04:59:48.72 AFV.select :carrierSolution
@04:59:48.82 delay 2 seconds
@04:59:50.85 AS.reconfigure ASconfig
@04:59:50.91 AS.seek 1ml
@05:00:48.66 delay 1 second
@05:00:49.68 AFV.select :holdingCoil
@05:00:49.78 delay 1 second
@05:00:50.81 AS.reconfigure ASfast
@05:00:50.86 AS.seek 0ml
@05:01:18.19 delay 3 seconds
@05:01:21.21 AFV.select :carrierSolution
@05:01:21.31 delay 2 seconds
@05:01:23.33 AS.reconfigure ASconfig
@05:01:23.40 AS.seek 1ml
@05:02:21.14 delay 1 second
@05:02:22.16 AFV.select :holdingCoil
@05:02:22.26 delay 1 second
@05:02:23.29 AS.reconfigure ASfast
@05:02:23.34 AS.seek 0ml
@05:02:50.67 delay 3 seconds
@05:02:53.69 AFV.select :carrierSolution
@05:02:53.79 delay 2 seconds
@05:02:55.81 AS.reconfigure ASconfig
@05:02:55.88 AS.seek 1ml
@05:03:53.63 delay 1 second
@05:03:54.65 AFV.select :holdingCoil
@05:03:54.75 delay 1 second
@05:03:55.78 AS.reconfigure ASfast
@05:03:55.83 AS.seek 0ml
@05:04:23.16 delay 3 seconds
@05:04:26.18 AFV.select :carrierSolution
@05:04:26.28 delay 2 seconds
@05:04:28.30 AS.reconfigure ASconfig
@05:04:28.72 AS.seek 1ml
@05:05:26.48 delay 1 second
@05:05:27.50 AFV.select :holdingCoil
@05:05:27.60 delay 1 second
@05:05:28.63 AS.reconfigure ASnorm
@05:05:28.68 AS.seek 0ml
@05:06:19.38 delay 5 seconds
@05:06:24.46 <bkha> Session Ended
@09:24:37.86CEST23-Apr-21 <MAIN> ESP2 v3.30 I2C Gateway v3.06 for core bus
@09:24:40.24 
Configuration for mer 7/20/20 brent@mbari.org
/home/esp/esp2/type/shallow/mer/configure.rb
@09:24:40.42 
Configuration for MFB3 by preston@mbari.org
/home/esp/esp2/type/mfb/MFB3/configure.rb
@09:24:45.73 Polling Can every 10:00
@09:24:47.45 CS.configure :default
@09:24:47.49 CS.hold
@09:24:47.55 CS.configure :default
@09:24:47.63 PS.configure :default
@09:24:47.76 SS.configure :default
@09:24:47.83 SS.hold
@09:24:47.93 SS.configure :default
@09:24:48.09 CC.configure :default
@09:24:48.17 CC.hold
@09:24:48.27 CC.configure :default
@09:24:48.43 PC.configure :default
@09:24:48.51 PC.hold
@09:24:48.58 PC.configure :default
@09:24:48.67 Forearm.configure :loose
@09:24:48.71 Forearm.hold
@09:24:48.75 Forearm.configure :loose
@09:24:48.83 Elbow.configure :loose
@09:24:48.87 Elbow.hold
@09:24:48.91 Elbow.configure :loose
@09:24:49.01 SC.configure :loose
@09:24:49.05 SC.hold
@09:24:49.09 SC.configure :loose
@09:24:49.17 SE.configure :default
@09:24:49.21 SE.hold
@09:24:49.27 SE.configure :default
@09:24:49.30 CH.coast
@09:24:49.35 CH.configure :default
@09:24:49.38 PH.coast
@09:24:49.43 PH.configure :default
@09:24:49.53 SPE.coast
@09:24:49.58 SPE.configure SPEconfig
@09:24:49.65 AS.configure ASconfig
@09:24:49.69 AS.hold
@09:24:49.74 AS.configure ASconfig
@09:24:49.78 PCR.off
@09:24:49.82 PCR.pidtemp= 50,0.1,0,300,25,25
@09:24:49.85 PCR.caltemp= 2.5051,0.0372,0
@09:24:49.88 All dwarf controllers running firmware version 3.75
@09:24:50.11 Cleanly exited at 09:23:58CEST23-Apr-21
@09:24:50.14 Waiting for espclients...
@09:24:50.82 <bkha> Session Begun
@09:24:57.36 -> showlog 100
@09:25:08.27 -> deconEluateRecoveryLine :extSrc
@09:25:08.29 deconEluateRecoveryLine extSrc
@09:25:08.31 ATV.dial :waste
@09:25:08.42 AS.reconfigure ASnorm
@09:25:08.49 AS.seek 0ml
@09:25:08.62 ATV.dial :air3
@09:25:09.51 AS.reconfigure ASslow1
@09:25:09.57 AS.seek 0.01ml
@09:25:11.90 delay 2 seconds
@09:25:13.92 ATV.dial :bleach
@09:25:14.40 AS.seek 0.31ml
@09:26:15.06 delay 3 seconds
@09:26:18.08 ATV.dial :air3
@09:26:18.67 delay 3 seconds
@09:26:21.74 AS.seek 0.335ml
@09:26:27.13 delay 3 seconds
@09:26:30.15 ATV.dial :bleach
@09:26:30.80 AS.seek 0.32ml
@09:26:34.09 delay 3 seconds
@09:26:37.11 ATV.dial :extSrc
@09:26:37.74 AS.seek 0ml
@09:27:42.42 AFV.select :carrierSolution
@09:27:42.54 delay 2 seconds
@09:27:44.57 AS.reconfigure ASconfig
@09:27:44.62 AS.seek 0.5ml
@09:28:13.65 delay 1 second
@09:28:14.67 AFV.select :holdingCoil
@09:28:14.77 ATV.dial :extSrc
@09:28:14.79 AS.reconfigure ASslow1
@09:28:14.86 AS.seek 0ml
@09:29:55.70 AFV.select :carrierSolution
@09:29:55.82 delay 2 seconds
@09:29:57.85 AS.reconfigure ASconfig
@09:29:57.90 AS.seek 0.5ml
@09:30:26.93 delay 1 second
@09:30:27.95 AFV.select :holdingCoil
@09:30:28.05 ATV.dial :extSrc
@09:30:28.07 AS.reconfigure ASslow1
@09:30:28.14 AS.seek 0ml
@09:32:09.12 AFV.select :carrierSolution
@09:32:09.24 delay 2 seconds
@09:32:11.27 AS.reconfigure ASconfig
@09:32:11.69 AS.seek 0.5ml
@09:32:40.71 delay 1 second
@09:32:41.73 AFV.select :holdingCoil
@09:32:41.83 ATV.dial :extSrc
@09:32:41.85 AS.reconfigure ASslow1
@09:32:41.92 AS.seek 0ml
@09:34:22.76 AFV.select :carrierSolution
@09:34:22.88 delay 2 seconds
@09:34:24.91 AS.reconfigure ASconfig
@09:34:24.96 AS.seek 0.5ml
@09:34:53.99 delay 1 second
@09:34:55.01 AFV.select :holdingCoil
@09:34:55.12 ATV.dial :extSrc
@09:34:55.14 AS.reconfigure ASslow1
@09:34:55.21 AS.seek 0ml
@09:36:36.06 ATV.dial :air2
@09:36:37.44 AS.reconfigure ASslow0
@09:36:37.50 AS.seek 0.5ml
@09:37:28.15 ATV.dial :extSrc
@09:37:29.71 AS.seek 0.02ml
@09:38:18.35 delay 1 second
@09:38:19.37 ATV.dial :waste
@09:38:20.73 AS.reconfigure ASconfig
@09:38:20.79 AS.seek 0ml
@09:46:06.16 -> AARHD
@09:46:06.18 NameError in bkha -- uninitialized constant AARHD
@09:46:20.52 -> AALPP3
@09:46:34.04 -> AARPPÂ4
@09:46:34.05 SyntaxError in bkha -- compile error
bkha:5: Invalid char `\302' in expression
@09:46:39.20 -> AARPP4
@10:10:37.84 Session Ended
@10:51:26.96 <bkha> Session Begun
@10:51:32.16 -> ATV.AR
@10:51:32.19 NoMethodError in bkha -- undefined method `AR' for ATV:Shaft
@10:51:34.11 -> ATV.ARV
@10:51:34.12 ATV.dial ARV
@10:51:39.82 -> AARPP3
@10:51:39.83 NameError in bkha -- uninitialized constant AARPP3
@10:51:51.70 -> PP3
@10:51:51.71 NameError in bkha -- uninitialized constant PP3
@10:51:57.01 -> ARV.legend
@10:52:04.26 -> PP3
@10:52:04.27 NameError in bkha -- uninitialized constant PP3
@10:52:13.98 -> AARPP4
@10:52:21.75 -> AALPP3
@10:52:36.32 -> ARV.air2
@10:52:36.34 ARV.dial :air2
@10:52:39.81 -> ATV
@10:52:49.38 -> AS.pull 0.5, ASslow1
@10:52:49.46 AS.reconfigure ASslow1
@10:52:49.52 AS.seek 0.5ml
@10:52:55.56 AS.hold
@10:52:55.79 UserAbort in bkha -- Analytical Syringe seek to 0.5ml Aborted!
@10:53:17.67 -> ARV.PP3
@10:53:17.68 ARV.dial :PP3
@10:53:37.92 -> AS.pull 0.006, ASslow2
@10:53:38.00 AS.reconfigure ASslow2
@10:53:38.06 AS.seek 0.036ml
@10:54:23.34 -> ARV.air2
@10:54:23.35 ARV.dial :air2
@10:54:33.75 -> AS.pull 0.02, ASslow2
@10:54:33.83 AS.seek 0.056ml
@10:55:04.96 -> ARV.PP4
@10:55:04.97 ARV.dial :PP4
@10:55:09.18 -> AS.pull 0.006, ASslow2
@10:55:09.26 AS.seek 0.062ml
@10:55:43.91 -> ARV.air3
@10:55:43.92 ARV.dial :air3
@10:55:50.09 -> AS.pull 0.02, ASslow2
@10:55:50.17 AS.seek 0.082ml
@10:56:24.50 -> ARV.MM2
@10:56:24.51 ARV.dial :MM2
@10:56:31.17 -> AS.pull 0.006, ASslow2
@10:56:31.25 AS.seek 0.088ml
@10:56:58.24 -> ARV.air2
@10:56:58.25 ARV.dial :air2
@10:57:01.74 -> AS.pull 0.02, ASslow2
@10:57:01.82 AS.seek 0.108ml
@10:57:29.29 -> pcrDecon
@10:57:29.36 Cleaning PCR
@10:57:29.37 AFV.select :carrierSolution
@10:57:29.50 delay 2 seconds
@10:57:31.52 AS.reconfigure ASconfig
@10:57:31.58 AS.seek 0.5ml
@10:57:54.44 delay 1 second
@10:57:55.46 AFV.select :holdingCoil
@10:57:55.56 ASV.dial :waste1
@10:57:55.65 ATV.dial :air3
@10:57:57.02 AS.reconfigure ASslow1
@10:57:57.08 AS.seek 0.51ml
@10:57:59.45 delay 2 seconds
@10:58:01.47 ATV.dial :bleach
@10:58:02.11 AS.seek 0.81ml
@10:59:02.69 delay 3 seconds
@10:59:05.71 ATV.dial :air3
@10:59:06.30 delay 3 seconds
@10:59:09.37 AS.seek 0.835ml
@10:59:14.65 delay 3 seconds
@10:59:17.67 ATV.dial :bleach
@10:59:18.32 AS.seek 0.82ml
@10:59:21.63 delay 3 seconds
@10:59:24.65 ARV.dial :waste
@10:59:26.22 delay 3 seconds
@10:59:29.24 ATV.dial :ARV
@10:59:30.16 AS.reconfigure ASconfig
@10:59:30.22 AS.seek 0.47ml
@10:59:50.63 AFV.select :carrierSolution
@10:59:50.74 delay 2 seconds
@10:59:52.77 AS.seek 1ml
@11:00:23.49 delay 1 second
@11:00:24.51 AFV.select :holdingCoil
@11:00:24.61 delay 1 second
@11:00:25.64 AS.reconfigure ASfast
@11:00:25.69 AS.seek 0ml
@11:00:53.04 AFV.select :carrierSolution
@11:00:53.15 delay 2 seconds
@11:00:55.17 AS.reconfigure ASconfig
@11:00:55.23 AS.seek 1ml
@11:01:52.97 delay 1 second
@11:01:53.99 AFV.select :holdingCoil
@11:01:54.09 delay 1 second
@11:01:55.12 AS.reconfigure ASfast
@11:01:55.17 AS.seek 0ml
@11:02:22.52 AFV.select :carrierSolution
@11:02:22.63 delay 2 seconds
@11:02:24.65 AS.reconfigure ASconfig
@11:02:24.71 AS.seek 1ml
@11:03:22.49 delay 1 second
@11:03:23.51 AFV.select :holdingCoil
@11:03:23.61 delay 1 second
@11:03:24.64 AS.reconfigure ASfast
@11:03:24.69 AS.seek 0ml
@11:03:52.02 ARV.dial between carrier and air1
@11:03:52.55 ATV.dial :air3
@11:03:53.92 AS.reconfigure ASslow1
@11:03:53.98 AS.seek 0.01ml
@11:03:56.57 delay 2 seconds
@11:03:58.59 ATV.dial :bleach
@11:03:59.25 AS.seek 0.81ml
@11:06:40.49 delay 3 seconds
@11:06:43.51 ATV.dial :air3
@11:06:44.11 delay 3 seconds
@11:06:47.18 AS.seek 0.835ml
@11:06:52.48 delay 3 seconds
@11:06:55.50 ATV.dial :bleach
@11:06:56.14 AS.seek 0.82ml
@11:06:59.44 delay 3 seconds
@11:07:02.46 ATV.dial PCR
@11:07:02.90 AS.reconfigure ASconfig
@11:07:02.96 AS.seek 0ml
@11:07:50.38 PCR.program :bleachProfile
@11:07:50.44 PCR program should complete in < 2:45
@11:07:50.46 PCR.run 10:00
@11:10:49.68 PCR.off
@11:10:49.72 AFV.select :carrierSolution
@11:10:49.83 delay 2 seconds
@11:10:51.86 AS.seek 1ml
@11:11:49.60 delay 1 second
@11:11:50.62 AFV.select :holdingCoil
@11:11:50.72 delay 1 second
@11:11:51.75 AS.reconfigure ASfast
@11:11:51.80 AS.seek 0ml
@11:12:19.16 delay 3 seconds
@11:12:22.18 AFV.select :carrierSolution
@11:12:22.28 delay 2 seconds
@11:12:24.30 AS.reconfigure ASconfig
@11:12:24.37 AS.seek 1ml
@11:13:22.11 delay 1 second
@11:13:23.13 AFV.select :holdingCoil
@11:13:23.23 delay 1 second
@11:13:24.25 AS.reconfigure ASfast
@11:13:24.31 AS.seek 0ml
@11:13:51.63 delay 3 seconds
@11:13:54.65 AFV.select :carrierSolution
@11:13:54.75 delay 2 seconds
@11:13:56.78 AS.reconfigure ASconfig
@11:13:56.84 AS.seek 1ml
@11:14:54.63 delay 1 second
@11:14:55.65 AFV.select :holdingCoil
@11:14:55.75 delay 1 second
@11:14:56.78 AS.reconfigure ASfast
@11:14:56.83 AS.seek 0ml
@11:15:24.18 delay 3 seconds
@11:15:27.20 AFV.select :carrierSolution
@11:15:27.30 delay 2 seconds
@11:15:29.32 AS.reconfigure ASconfig
@11:15:29.39 AS.seek 1ml
@11:16:27.18 delay 1 second
@11:16:28.20 AFV.select :holdingCoil
@11:16:28.30 delay 1 second
@11:16:29.32 AS.reconfigure ASfast
@11:16:29.38 AS.seek 0ml
@11:16:56.72 delay 3 seconds
@11:16:59.74 AFV.select :carrierSolution
@11:16:59.84 delay 2 seconds
@11:17:01.86 AS.reconfigure ASconfig
@11:17:01.93 AS.seek 1ml
@11:17:59.68 delay 1 second
@11:18:00.70 AFV.select :holdingCoil
@11:18:00.80 delay 1 second
@11:18:01.82 AS.reconfigure ASnorm
@11:18:01.88 AS.seek 0ml
@13:11:30.14 -> ATV.extSrc
@13:11:30.16 ATV.dial :extSrc
@13:11:35.26 -> AS.pull 0.210, ASslow1
@13:11:35.35 AS.reconfigure ASslow1
@13:11:35.41 AS.seek 0.21ml
@13:12:34.58 -> ATV.air1
@13:12:34.59 ATV.dial :air1
@13:12:40.60 -> AS.pull 0.03, ASslow1
@13:12:40.68 AS.seek 0.24ml
@13:12:44.85 AS.hold
@13:12:45.08 UserAbort in bkha -- Analytical Syringe seek to 0.24ml Aborted!
@13:12:52.02 -> ATV.waste
@13:12:52.03 ATV.dial :waste
@13:12:54.34 -> AS.empty
@13:12:54.36 AS.reconfigure ASconfig
@13:12:54.43 AS.seek 0ml
@13:13:11.37 -> exit
@13:13:11.38 Session Ended
Log::Locked in trapHandler -- Another process is already writing to /var/log/esp/real.log
@13:13:30.30 <bkha> Session Begun
@13:13:40.69 -> showlog 50
@13:14:00.94 -> abort! "a"
@13:14:00.95 CmdServer::Client::Error in bkha -- Aborting Mission: a
@13:14:01.01 <Qmail> BadNews.email "a",Subject: "Mission ABORTED!"
@13:14:10.35 <bkha> Session Ended
@13:14:23.37CEST23-Apr-21 <MAIN> ESP2 v3.30 I2C Gateway v3.06 for core bus
@13:14:25.84 
Configuration for mer 7/20/20 brent@mbari.org
/home/esp/esp2/type/shallow/mer/configure.rb
@13:14:25.93 
Configuration for MFB3 by preston@mbari.org
/home/esp/esp2/type/mfb/MFB3/configure.rb
@13:14:31.88 Polling Can every 10:00
@13:14:34.00 CS.configure :default
@13:14:34.05 CS.hold
@13:14:34.12 CS.configure :default
@13:14:34.20 PS.configure :default
@13:14:34.28 SS.configure :default
@13:14:34.32 SS.hold
@13:14:34.36 SS.configure :default
@13:14:34.44 CC.configure :default
@13:14:34.48 CC.hold
@13:14:34.56 CC.configure :default
@13:14:34.70 PC.configure :default
@13:14:34.78 PC.hold
@13:14:34.88 PC.configure :default
@13:14:35.06 Forearm.configure :loose
@13:14:35.14 Forearm.hold
@13:14:35.22 Forearm.configure :loose
@13:14:35.38 Elbow.configure :loose
@13:14:35.43 Elbow.hold
@13:14:35.49 Elbow.configure :loose
@13:14:35.58 SC.configure :loose
@13:14:35.62 SC.hold
@13:14:35.67 SC.configure :loose
@13:14:35.75 SE.configure :default
@13:14:35.79 SE.hold
@13:14:35.85 SE.configure :default
@13:14:35.88 CH.coast
@13:14:35.95 CH.configure :default
@13:14:36.01 PH.coast
@13:14:36.11 PH.configure :default
@13:14:36.31 SPE.coast
@13:14:36.39 SPE.configure SPEconfig
@13:14:36.55 AS.configure ASconfig
@13:14:36.61 AS.hold
@13:14:36.71 AS.configure ASconfig
@13:14:36.76 PCR.off
@13:14:36.80 PCR.pidtemp= 50,0.1,0,300,25,25
@13:14:36.83 PCR.caltemp= 2.5051,0.0372,0
@13:14:36.86 All dwarf controllers running firmware version 3.75
@13:14:37.08 Cleanly exited at 13:14:10CEST23-Apr-21
@13:14:37.11 Loading /home/esp/esp2/mission/GENOMAP_v3.rb mission
@13:14:37.30 <MFBPCR> Extracting Nucleic Acid from extSrc
@13:14:37.35 SPE.hold 2:46:40,55C
@13:14:37.43 ASV.dial :SPEdelivery
@13:14:37.89 delay 20 seconds
@13:14:37.92 <bkha> Session Begun
@13:14:57.93 <MFBPCR> AFV.select :holdingCoil
@13:14:58.04 ATV.dial :air2
@13:14:59.51 AS.reconfigure ASslow1
@13:14:59.57 AS.seek 0.07ml
@13:15:13.98 delay 3 seconds
@13:15:17.00 ATV.dial :extSrc
@13:15:18.53 AS.seek 0.47ml
@13:16:39.25 delay 5 seconds
@13:16:44.32 Solid Phase Extraction Heater at 40.6C
@13:16:44.33 ATV.dial ASV
@13:16:45.22 ASV.dial :SPEdelivery
@13:16:45.30 AS.seek 0.042ml
@13:18:11.77 delay 10 seconds
@13:18:21.80 Rinsing external lysate line extSrc
@13:18:21.81 AFV.select :carrierSolution
@13:18:21.93 delay 2 seconds
@13:18:23.96 AS.reconfigure ASconfig
@13:18:24.01 AS.seek 1ml
@13:19:19.37 delay 1 second
@13:19:20.39 AFV.select :holdingCoil
@13:19:20.50 delay 1 second
@13:19:21.52 ATV.dial :extSrc
@13:19:22.34 AS.reconfigure ASnorm
@13:19:22.40 AS.seek 0ml
@13:20:13.09 ATV.dial :air2
@13:20:14.67 AS.reconfigure ASslow0
@13:20:14.73 AS.seek 0.05ml
@13:20:20.12 ATV.dial :extSrc
@13:20:21.70 AS.seek 0.01ml
@13:20:26.08 ATV.dial :waste
@13:20:27.52 AS.reconfigure ASconfig
@13:20:27.58 AS.seek 0ml
@13:20:28.47 ethanol wash
@13:20:28.48 ATV.dial :air2
@13:20:29.67 AS.reconfigure ASslow2
@13:20:29.72 AS.seek 0.01ml
@13:20:40.14 delay 3 seconds
@13:20:43.16 Pulling 0.4ml of Ethanol Wash
@13:20:43.17 ATV.dial :ethanol
@13:20:44.06 AS.reconfigure ASslow1
@13:20:44.11 AS.seek 0.41ml
@13:22:05.00 delay 3 seconds
@13:22:08.02 ATV.dial :air1
@13:22:08.45 delay 3 seconds
@13:22:11.52 AS.seek 0.435ml
@13:22:16.85 delay 3 seconds
@13:22:19.87 ATV.dial :ethanol
@13:22:20.51 AS.seek 0.42ml
@13:22:23.81 delay 3 seconds
@13:22:26.83 ATV.dial ASV
@13:22:28.24 AS.seek 0.093ml
@13:23:34.30 delay 3 seconds
@13:23:37.32 ATV.dial :waste2
@13:23:38.12 AS.reconfigure ASconfig
@13:23:38.18 AS.seek 0ml
@13:23:43.83 ATV.dial :air2
@13:23:44.25 AS.reconfigure ASslow1
@13:23:44.31 AS.seek 1ml
@13:27:05.70 delay 5 seconds
@13:27:10.72 ATV.dial ASV
@13:27:11.77 ASV.dial :SPEdelivery
@13:27:11.79 AS.seek 0.02ml
@13:30:29.17 delay 5 seconds
@13:30:34.19 ATV.dial :air2
@13:30:35.28 AS.seek 0.52ml
@13:32:16.16 delay 5 seconds
@13:32:21.18 ATV.dial ASV
@13:32:22.25 ASV.dial :wasteR
@13:32:22.64 AS.seek 0.3ml
@13:33:07.23 delay 20 seconds
@13:33:27.27 AS.seek 0.07ml
@13:34:13.81 delay 20 seconds
@13:34:33.83 ATV.dial :waste2
@13:34:34.64 AS.reconfigure ASconfig
@13:34:34.70 AS.seek 0ml
@13:34:39.03 Elution
@13:34:39.04 ATV.dial :air1
@13:34:40.24 AS.reconfigure ASslow1
@13:34:40.30 AS.seek 0.05ml
@13:34:50.80 delay 3 seconds
@13:34:53.82 ATV.dial :ElutionWater
@13:34:54.52 AS.seek 0.11ml
@13:35:06.92 delay 3 seconds
@13:35:09.94 ATV.dial ASV,via: :air2
@13:35:12.18 ASV.dial :SPEelution
@13:35:12.80 AFV.select :carrierSolution
@13:35:12.96 AS.reconfigure ASnorm
@13:35:13.01 AS.seek 0.76ml
@13:35:46.06 delay 3 seconds
@13:35:49.08 AFV.select :holdingCoil
@13:35:49.23 AS.reconfigure ASslow1
@13:35:49.28 AS.seek 0.475ml
@13:36:46.89 delay 5 seconds
@13:36:51.97 AS.reconfigure ASslow2
@13:36:52.03 AS.seek 0.44ml
@13:37:27.81 delay 1:00
@13:38:27.85 pushing eluate to holding coil
@13:38:27.92 AS.seek 0.28ml
@13:41:09.06 delay 30 seconds
@13:41:39.09 ATV.dial :waste,via: :air3
@13:41:40.92 AS.reconfigure ASconfig
@13:41:40.98 AS.seek 0ml
@13:41:57.39 Parking Bleach in LysateSource line and SPEcolumn
@13:41:57.40 ATV.dial :air3
@13:41:58.30 AS.reconfigure ASslow1
@13:41:58.36 AS.seek 0.03ml
@13:42:04.67 ATV.dial :ElutionWater
@13:42:05.32 AS.seek 0.06ml
@13:42:11.70 ATV.dial :air3
@13:42:12.31 AS.seek 0.07ml
@13:42:14.62 delay 2 seconds
@13:42:16.64 ATV.dial :bleach
@13:42:17.14 AS.seek 0.15ml
@13:42:33.56 delay 3 seconds
@13:42:36.58 ATV.dial :air3
@13:42:37.19 delay 3 seconds
@13:42:40.26 AS.seek 0.175ml
@13:42:45.51 delay 3 seconds
@13:42:48.54 ATV.dial :bleach
@13:42:49.20 AS.seek 0.16ml
@13:42:52.50 delay 3 seconds
@13:42:55.52 ATV.dial :extSrc
@13:42:56.17 AS.reconfigure ASconfig
@13:42:56.23 AS.seek 0.015ml
@13:43:04.84 ATV.dial :waste
@13:43:06.39 AS.seek 0ml
@13:43:07.55 ATV.dial :air3
@13:43:08.42 AS.reconfigure ASslow1
@13:43:08.48 AS.seek 0.01ml
@13:43:10.87 delay 2 seconds
@13:43:12.89 ATV.dial :bleach
@13:43:13.37 AS.seek 0.58ml
@13:45:08.28 delay 3 seconds
@13:45:11.30 ATV.dial :air3
@13:45:11.91 delay 3 seconds
@13:45:14.98 AS.seek 0.605ml
@13:45:20.31 delay 3 seconds
@13:45:23.33 ATV.dial :bleach
@13:45:23.97 AS.seek 0.59ml
@13:45:27.23 delay 3 seconds
@13:45:30.25 ATV.dial :ASV
@13:45:31.36 ASV.dial :wasteR
@13:45:31.98 AS.seek 0ml
@13:47:30.83 delay 1:00
@13:48:30.86 AFV.select :carrierSolution
@13:48:30.97 delay 2 seconds
@13:48:32.99 AS.reconfigure ASconfig
@13:48:33.05 AS.seek 1ml
@13:49:30.80 delay 1 second
@13:49:31.82 AFV.select :holdingCoil
@13:49:31.92 delay 1 second
@13:49:32.94 ATV.dial ASV
@13:49:32.95 ASV.dial :wasteR
@13:49:32.97 AS.reconfigure ASslow1
@13:49:33.04 AS.seek 0ml
@13:52:54.42 delay 20 seconds
@13:53:14.45 ATV.dial :air3
@13:53:16.09 AS.seek 0.59ml
@13:55:15.12 delay 3 seconds
@13:55:18.14 ATV.dial :ASV
@13:55:19.65 ASV.dial :wasteR
@13:55:19.73 AS.seek 0.09ml
@13:57:00.58 delay 30 seconds
@13:57:30.61 ATV.dial :extSrc
@13:57:31.44 delay 3 seconds
@13:57:34.46 ATV.dial :ElutionWater
@13:57:35.65 AS.seek 0.12ml
@13:57:42.08 ATV.dial :air3
@13:57:42.73 AS.seek 0.13ml
@13:57:45.11 delay 2 seconds
@13:57:47.13 ATV.dial :bleach
@13:57:47.64 AS.seek 0.23ml
@13:58:08.12 delay 3 seconds
@13:58:11.14 ATV.dial :air3
@13:58:11.75 delay 3 seconds
@13:58:14.82 AS.seek 0.255ml
@13:58:20.19 delay 3 seconds
@13:58:23.21 ATV.dial :bleach
@13:58:24.31 AS.seek 0.24ml
@13:58:27.56 delay 3 seconds
@13:58:30.58 ATV.dial :ASV
@13:58:31.69 ASV.dial :SPEdelivery
@13:58:32.09 AFV.select :carrierSolution
@13:58:32.20 delay 2 seconds
@13:58:34.23 AS.reconfigure ASconfig
@13:58:34.28 AS.seek 0.5ml
@13:58:49.55 delay 1 second
@13:58:50.57 AFV.select :holdingCoil
@13:58:50.72 AS.reconfigure ASslow1
@13:58:50.77 AS.seek 0.17ml
@13:59:57.45 SPE.hold 2:46:40,95C
@13:59:57.50 delay 3:00
@14:02:57.55 AFV.select :carrierSolution
@14:02:57.66 delay 2 seconds
@14:02:59.68 AS.reconfigure ASconfig
@14:02:59.74 AS.seek 1ml
@14:03:47.73 delay 1 second
@14:03:48.75 AFV.select :holdingCoil
@14:03:48.85 delay 1 second
@14:03:49.87 ASV.dial :wasteD
@14:03:50.98 AS.seek 0ml
@14:04:48.75 Priming Eluate
@14:04:48.76 AFV.select :carrierSolution
@14:04:48.88 delay 2 seconds
@14:04:50.91 AS.seek 0.5ml
@14:05:19.98 delay 1 second
@14:05:21.00 AFV.select :holdingCoil
@14:05:21.10 ATV.dial ASV
@14:05:21.12 ASV.dial :SPErecovery
@14:05:21.76 Swish #1 of 2
@14:05:21.84 AS.reconfigure ASslow0
@14:05:21.90 AS.seek 0.6ml
@14:05:32.37 AS.seek 0.5ml
@14:05:42.75 Swish #2 of 2
@14:05:42.83 AS.seek 0.6ml
@14:05:53.33 AS.seek 0.5ml
@14:06:03.69 delay 3 seconds
@14:06:06.76 AS.reconfigure ASslow1
@14:06:06.82 AS.seek 0.684ml
@14:06:44.15 delay 3 seconds
@14:06:47.17 Eluate primed
@14:06:47.18 ATV.dial :waste2
@14:06:47.86 AS.reconfigure ASnorm
@14:06:47.92 AS.seek 0ml
@14:07:22.73 SPE.coast
@14:07:22.81 ASV.dial :SPErecovery
@14:07:22.83 Setting up PCR for SYBRIPc -- writing /var/log/esp/SYBRIPc21apr2314h.pcr
@14:07:22.85 Building PCR Slug from ASV with MM1 mix, PP6 primer
@14:07:22.87 Priming with carrier
@14:07:22.89 ATV.dial ARV
@14:07:23.95 ARV.dial :carrier
@14:07:24.31 AS.seek 0.5ml
@14:07:49.87 AFV.select :carrierSolution
@14:07:49.99 delay 2 seconds
@14:07:52.02 AS.reconfigure ASconfig
@14:07:52.08 AS.seek 1ml
@14:08:21.09 delay 1 second
@14:08:22.11 AFV.select :holdingCoil
@14:08:22.21 delay 1 second
@14:08:23.23 ATV.dial :waste
@14:08:24.86 AS.reconfigure ASnorm
@14:08:24.92 AS.seek 0ml
@14:09:15.62 AFV.select :carrierSolution
@14:09:15.74 delay 2 seconds
@14:09:17.77 AS.reconfigure ASconfig
@14:09:17.82 AS.seek 0.5ml
@14:09:46.90 delay 1 second
@14:09:47.92 AFV.select :holdingCoil
@14:09:48.02 Building Slug
@14:09:48.04 ATV.dial :ARV
@14:09:49.79 delay 2 seconds
@14:09:51.81 ARV.dial :air1
@14:09:52.23 delay 2 seconds
@14:09:54.30 AS.reconfigure ASslow2
@14:09:54.37 AS.seek 0.506ml
@14:10:00.73 delay 2 seconds
@14:10:02.75 ARV.dial :MM1
@14:10:03.39 delay 2 seconds
@14:10:05.41 Adding MM1
@14:10:05.49 AS.seek 0.524ml
@14:10:23.78 delay 25 seconds
@14:10:48.80 ARV.dial :PP6
@14:10:50.14 Adding PP6
@14:10:50.15 delay 2 seconds
@14:10:52.23 AS.seek 0.53ml
@14:10:58.65 delay 10 seconds
@14:11:08.68 ARV.dial :air1
@14:11:09.56 adding ARV air
@14:11:09.57 delay 2 seconds
@14:11:11.65 AS.seek 0.536ml
@14:11:18.22 delay 5 seconds
@14:11:23.24 ARV.dial :carrier
@14:11:23.65 delay 7 seconds
@14:11:30.73 AS.seek 0.594ml
@14:12:29.39 delay 5 seconds
@14:12:34.41 ATV.dial :ASV
@14:12:34.83 delay 2 seconds
@14:12:36.90 AS.seek 0.6ml
@14:12:43.42 delay 4 seconds
@14:12:47.44 ATV.dial ARV
@14:12:48.01 delay 2 seconds
@14:12:50.03 ARV.dial :carrier
@14:12:50.04 delay 2 seconds
@14:12:52.12 AS.seek 0.65ml
@14:13:42.84 delay 4 seconds
@14:13:46.86 ATV.dial PCR
@14:13:47.51 ASV.dial :waste1
@14:13:48.34 ARV.dial :air1
@14:13:48.75 Positioning Slug by 0.324ml
@14:13:48.84 AS.seek 0.326ml
@14:19:15.39 delay 5 seconds
@14:19:20.41 ATV.dial ARV
@14:19:21.23 ASV.dial :PCRlock
@14:19:21.62 delay 5 seconds
@14:19:26.64 Running SYBRIPc Profile60CIQ1 thermocycling
@14:19:26.66 <PCR> PCR.program Profile60CIQ1
@14:19:26.77 Profile60CIQ1 should complete in < 1:56:15
@14:19:26.78 PCR.run 2:30:18.75
@15:59:09.80 PCR.off
@15:59:10.35 <MFBPCR> PCR for SYBRIPc completed -- closed /var/log/esp/SYBRIPc21apr2314h.pcr
@15:59:10.43 Cleaning PCR
@15:59:10.44 AFV.select :carrierSolution
@15:59:10.56 delay 2 seconds
@15:59:12.59 AS.reconfigure ASconfig
@15:59:12.64 AS.seek 0.5ml
@15:59:22.93 delay 1 second
@15:59:23.95 AFV.select :holdingCoil
@15:59:24.05 ASV.dial :waste1
@15:59:24.45 ATV.dial :air3
@15:59:25.81 AS.reconfigure ASslow1
@15:59:25.87 AS.seek 0.51ml
@15:59:28.27 delay 2 seconds
@15:59:30.29 ATV.dial :bleach
@15:59:30.94 AS.seek 0.81ml
@16:00:31.67 delay 3 seconds
@16:00:34.69 ATV.dial :air3
@16:00:35.28 delay 3 seconds
@16:00:38.35 AS.seek 0.835ml
@16:00:43.67 delay 3 seconds
@16:00:46.69 ATV.dial :bleach
@16:00:47.34 AS.seek 0.82ml
@16:00:50.63 delay 3 seconds
@16:00:53.65 ARV.dial :waste
@16:00:54.29 delay 3 seconds
@16:00:57.31 ATV.dial :ARV
@16:00:58.23 AS.reconfigure ASconfig
@16:00:58.29 AS.seek 0.47ml
@16:01:18.70 AFV.select :carrierSolution
@16:01:18.81 delay 2 seconds
@16:01:21.23 AS.seek 1ml
@16:01:52.02 delay 1 second
@16:01:53.04 AFV.select :holdingCoil
@16:01:53.14 delay 1 second
@16:01:54.17 AS.reconfigure ASfast
@16:01:54.22 AS.seek 0ml
@16:02:21.56 AFV.select :carrierSolution
@16:02:21.67 delay 2 seconds
@16:02:23.70 AS.reconfigure ASconfig
@16:02:23.75 AS.seek 1ml
@16:03:21.49 delay 1 second
@16:03:22.51 AFV.select :holdingCoil
@16:03:22.61 delay 1 second
@16:03:23.64 AS.reconfigure ASfast
@16:03:23.69 AS.seek 0ml
@16:03:51.02 AFV.select :carrierSolution
@16:03:51.13 delay 2 seconds
@16:03:53.16 AS.reconfigure ASconfig
@16:03:53.21 AS.seek 1ml
@16:04:51.01 delay 1 second
@16:04:52.03 AFV.select :holdingCoil
@16:04:52.13 delay 1 second
@16:04:53.16 AS.reconfigure ASfast
@16:04:53.21 AS.seek 0ml
@16:05:20.57 ARV.dial between carrier and air1
@16:05:21.09 ATV.dial :air3
@16:05:22.44 AS.reconfigure ASslow1
@16:05:22.50 AS.seek 0.01ml
@16:05:24.81 delay 2 seconds
@16:05:26.83 ATV.dial :bleach
@16:05:27.48 AS.seek 0.81ml
@16:08:08.64 delay 3 seconds
@16:08:11.67 ATV.dial :air3
@16:08:12.26 delay 3 seconds
@16:08:15.33 AS.seek 0.835ml
@16:08:20.63 delay 3 seconds
@16:08:23.65 ATV.dial :bleach
@16:08:24.29 AS.seek 0.82ml
@16:08:27.74 delay 3 seconds
@16:08:30.76 ATV.dial PCR
@16:08:31.20 AS.reconfigure ASconfig
@16:08:31.26 AS.seek 0ml
@16:09:18.68 PCR.program :bleachProfile
@16:09:18.74 PCR program should complete in < 2:45
@16:09:18.75 PCR.run 10:00
@16:12:14.54 PCR.off
@16:12:14.58 AFV.select :carrierSolution
@16:12:14.69 delay 2 seconds
@16:12:16.72 AS.seek 1ml
@16:13:14.51 delay 1 second
@16:13:15.54 AFV.select :holdingCoil
@16:13:15.68 delay 1 second
@16:13:16.71 AS.reconfigure ASfast
@16:13:16.77 AS.seek 0ml
@16:13:44.12 delay 3 seconds
@16:13:47.14 AFV.select :carrierSolution
@16:13:47.24 delay 2 seconds
@16:13:49.27 AS.reconfigure ASconfig
@16:13:49.34 AS.seek 1ml
@16:14:47.09 delay 1 second
@16:14:48.11 AFV.select :holdingCoil
@16:14:48.21 delay 1 second
@16:14:49.24 AS.reconfigure ASfast
@16:14:49.29 AS.seek 0ml
@16:15:16.63 delay 3 seconds
@16:15:19.65 AFV.select :carrierSolution
@16:15:19.75 delay 2 seconds
@16:15:21.77 AS.reconfigure ASconfig
@16:15:21.84 AS.seek 1ml
@16:16:19.59 delay 1 second
@16:16:20.61 AFV.select :holdingCoil
@16:16:20.71 delay 1 second
@16:16:21.74 AS.reconfigure ASfast
@16:16:21.79 AS.seek 0ml
@16:16:49.13 delay 3 seconds
@16:16:52.15 AFV.select :carrierSolution
@16:16:52.25 delay 2 seconds
@16:16:54.27 AS.reconfigure ASconfig
@16:16:54.34 AS.seek 1ml
@16:17:52.13 delay 1 second
@16:17:53.15 AFV.select :holdingCoil
@16:17:53.25 delay 1 second
@16:17:54.28 AS.reconfigure ASfast
@16:17:54.33 AS.seek 0ml
@16:18:21.66 delay 3 seconds
@16:18:24.68 AFV.select :carrierSolution
@16:18:24.78 delay 2 seconds
@16:18:26.80 AS.reconfigure ASconfig
@16:18:26.87 AS.seek 1ml
@16:19:24.62 delay 1 second
@16:19:25.64 AFV.select :holdingCoil
@16:19:25.74 delay 1 second
@16:19:26.76 AS.reconfigure ASnorm
@16:19:26.82 AS.seek 0ml
@16:20:17.53 Setting up PCR for ACYPP2 -- writing /var/log/esp/ACYPP221apr2316h.pcr
@16:20:17.55 Building PCR Slug from ASV with MM1 mix, PP2 primer
@16:20:17.57 Priming with carrier
@16:20:17.59 ATV.dial ARV
@16:20:18.66 ARV.dial :carrier
@16:20:19.01 AS.seek 0.5ml
@16:20:44.56 AFV.select :carrierSolution
@16:20:44.68 delay 2 seconds
@16:20:46.71 AS.reconfigure ASconfig
@16:20:46.77 AS.seek 1ml
@16:21:15.77 delay 1 second
@16:21:16.79 AFV.select :holdingCoil
@16:21:16.89 delay 1 second
@16:21:17.91 ATV.dial :waste
@16:21:19.70 AS.reconfigure ASnorm
@16:21:19.77 AS.seek 0ml
@16:22:10.45 AFV.select :carrierSolution
@16:22:10.57 delay 2 seconds
@16:22:12.60 AS.reconfigure ASconfig
@16:22:12.65 AS.seek 0.5ml
@16:22:41.71 delay 1 second
@16:22:42.73 AFV.select :holdingCoil
@16:22:42.83 Building Slug
@16:22:42.85 ATV.dial :ARV
@16:22:44.59 delay 2 seconds
@16:22:46.61 ARV.dial :air1
@16:22:47.02 delay 2 seconds
@16:22:49.09 AS.reconfigure ASslow2
@16:22:49.15 AS.seek 0.506ml
@16:22:55.37 delay 2 seconds
@16:22:57.39 ARV.dial :MM1
@16:22:58.02 delay 2 seconds
@16:23:00.04 Adding MM1
@16:23:00.12 AS.seek 0.524ml
@16:23:18.54 delay 25 seconds
@16:23:43.56 ARV.dial :PP2
@16:23:43.99 Adding PP2
@16:23:44.00 delay 2 seconds
@16:23:46.08 AS.seek 0.53ml
@16:23:52.57 delay 10 seconds
@16:24:02.60 ARV.dial :air2
@16:24:03.02 adding ARV air
@16:24:03.03 delay 2 seconds
@16:24:05.11 AS.seek 0.536ml
@16:24:12.12 delay 5 seconds
@16:24:17.14 ARV.dial :carrier
@16:24:18.49 delay 7 seconds
@16:24:25.57 AS.seek 0.594ml
@16:25:24.56 delay 5 seconds
@16:25:29.58 ATV.dial :ASV
@16:25:30.01 delay 2 seconds
@16:25:32.08 AS.seek 0.6ml
@16:25:38.34 delay 4 seconds
@16:25:42.36 ATV.dial ARV
@16:25:42.96 delay 2 seconds
@16:25:44.98 ARV.dial :carrier
@16:25:44.99 delay 2 seconds
@16:25:47.07 AS.seek 0.65ml
@16:26:37.66 delay 4 seconds
@16:26:41.68 ATV.dial PCR
@16:26:42.34 ASV.dial :waste1
@16:26:42.35 ARV.dial :air1
@16:26:42.77 Positioning Slug by 0.324ml
@16:26:42.86 AS.seek 0.326ml
@16:32:08.63 delay 5 seconds
@16:32:13.65 ATV.dial ARV
@16:32:14.46 ASV.dial :PCRlock
@16:32:14.87 delay 5 seconds
@16:32:19.89 Running ACYPP2 Profile60CIQ2 thermocycling
@16:32:19.91 <PCR> PCR.program Profile60CIQ2
@16:32:20.02 Profile60CIQ2 should complete in < 1:47:50
@16:32:20.03 PCR.run 2:19:47.5
@18:01:25.00 PCR.off
@18:01:25.63 <MFBPCR> Running ACYPP2 MeltProfile60 thermocycling
@18:01:25.64 <PCR> PCR.program MeltProfile60
@18:01:25.74 MeltProfile60 should complete in < 11:55
@18:01:25.75 PCR.run 19:53.75
@18:09:21.82 PCR.off
@18:09:22.75 <MFBPCR> PCR for ACYPP2 completed -- closed /var/log/esp/ACYPP221apr2316h.pcr
@18:09:22.82 Cleaning PCR
@18:09:22.83 AFV.select :carrierSolution
@18:09:22.95 delay 2 seconds
@18:09:24.98 AS.reconfigure ASconfig
@18:09:25.03 AS.seek 0.5ml
@18:09:35.38 delay 1 second
@18:09:36.40 AFV.select :holdingCoil
@18:09:36.50 ASV.dial :waste1
@18:09:36.90 ATV.dial :air3
@18:09:38.24 AS.reconfigure ASslow1
@18:09:38.30 AS.seek 0.51ml
@18:09:40.61 delay 2 seconds
@18:09:42.63 ATV.dial :bleach
@18:09:43.28 AS.seek 0.81ml
@18:10:43.93 delay 3 seconds
@18:10:46.95 ATV.dial :air3
@18:10:47.54 delay 3 seconds
@18:10:50.61 AS.seek 0.835ml
@18:10:56.00 delay 3 seconds
@18:10:59.02 ATV.dial :bleach
@18:10:59.68 AS.seek 0.82ml
@18:11:02.97 delay 3 seconds
@18:11:05.99 ARV.dial :waste
@18:11:06.63 delay 3 seconds
@18:11:09.65 ATV.dial :ARV
@18:11:10.57 AS.reconfigure ASconfig
@18:11:10.63 AS.seek 0.47ml
@18:11:31.04 AFV.select :carrierSolution
@18:11:31.15 delay 2 seconds
@18:11:33.18 AS.seek 1ml
@18:12:03.97 delay 1 second
@18:12:04.99 AFV.select :holdingCoil
@18:12:05.09 delay 1 second
@18:12:06.12 AS.reconfigure ASfast
@18:12:06.17 AS.seek 0ml
@18:12:33.52 AFV.select :carrierSolution
@18:12:33.63 delay 2 seconds
@18:12:35.65 AS.reconfigure ASconfig
@18:12:35.71 AS.seek 1ml
@18:13:33.51 delay 1 second
@18:13:34.53 AFV.select :holdingCoil
@18:13:34.63 delay 1 second
@18:13:35.66 AS.reconfigure ASfast
@18:13:35.71 AS.seek 0ml
@18:14:03.02 AFV.select :carrierSolution
@18:14:03.13 delay 2 seconds
@18:14:05.15 AS.reconfigure ASconfig
@18:14:05.21 AS.seek 1ml
@18:15:02.97 delay 1 second
@18:15:03.99 AFV.select :holdingCoil
@18:15:04.09 delay 1 second
@18:15:05.12 AS.reconfigure ASfast
@18:15:05.17 AS.seek 0ml
@18:15:32.51 ARV.dial between carrier and air1
@18:15:33.04 ATV.dial :air3
@18:15:34.38 AS.reconfigure ASslow1
@18:15:34.44 AS.seek 0.01ml
@18:15:36.83 delay 2 seconds
@18:15:38.85 ATV.dial :bleach
@18:15:39.50 AS.seek 0.81ml
@18:18:20.70 delay 3 seconds
@18:18:23.78 ATV.dial :air3
@18:18:24.36 delay 3 seconds
@18:18:27.43 AS.seek 0.835ml
@18:18:32.71 delay 3 seconds
@18:18:35.73 ATV.dial :bleach
@18:18:36.36 AS.seek 0.82ml
@18:18:39.65 delay 3 seconds
@18:18:42.67 ATV.dial PCR
@18:18:43.10 AS.reconfigure ASconfig
@18:18:43.16 AS.seek 0ml
@18:19:30.57 PCR.program :bleachProfile
@18:19:30.64 PCR program should complete in < 2:45
@18:19:30.65 PCR.run 10:00
@18:22:26.85 PCR.off
@18:22:26.89 AFV.select :carrierSolution
@18:22:27.00 delay 2 seconds
@18:22:29.03 AS.seek 1ml
@18:23:26.82 delay 1 second
@18:23:27.84 AFV.select :holdingCoil
@18:23:27.94 delay 1 second
@18:23:28.97 AS.reconfigure ASfast
@18:23:29.02 AS.seek 0ml
@18:23:56.38 delay 3 seconds
@18:23:59.40 AFV.select :carrierSolution
@18:23:59.50 delay 2 seconds
@18:24:01.52 AS.reconfigure ASconfig
@18:24:01.58 AS.seek 1ml
@18:24:59.32 delay 1 second
@18:25:00.34 AFV.select :holdingCoil
@18:25:00.44 delay 1 second
@18:25:01.47 AS.reconfigure ASfast
@18:25:01.52 AS.seek 0ml
@18:25:28.86 delay 3 seconds
@18:25:31.88 AFV.select :carrierSolution
@18:25:31.98 delay 2 seconds
@18:25:34.00 AS.reconfigure ASconfig
@18:25:34.07 AS.seek 1ml
@18:26:31.83 delay 1 second
@18:26:32.85 AFV.select :holdingCoil
@18:26:32.95 delay 1 second
@18:26:33.98 AS.reconfigure ASfast
@18:26:34.03 AS.seek 0ml
@18:27:01.38 delay 3 seconds
@18:27:04.40 AFV.select :carrierSolution
@18:27:04.50 delay 2 seconds
@18:27:06.52 AS.reconfigure ASconfig
@18:27:06.58 AS.seek 1ml
@18:28:04.33 delay 1 second
@18:28:05.35 AFV.select :holdingCoil
@18:28:05.45 delay 1 second
@18:28:06.48 AS.reconfigure ASfast
@18:28:06.53 AS.seek 0ml
@18:28:33.88 delay 3 seconds
@18:28:36.90 AFV.select :carrierSolution
@18:28:37.00 delay 2 seconds
@18:28:39.02 AS.reconfigure ASconfig
@18:28:39.09 AS.seek 1ml
@18:29:36.83 delay 1 second
@18:29:37.85 AFV.select :holdingCoil
@18:29:37.95 delay 1 second
@18:29:38.98 AS.reconfigure ASnorm
@18:29:39.03 AS.seek 0ml
@18:30:29.74 Setting up PCR for AOPP1 -- writing /var/log/esp/AOPP121apr2318h.pcr
@18:30:29.76 Building PCR Slug from ASV with MM1 mix, PP1 primer
@18:30:29.78 Priming with carrier
@18:30:29.80 ATV.dial ARV
@18:30:30.44 ARV.dial :carrier
@18:30:30.80 AS.seek 0.5ml
@18:30:56.33 AFV.select :carrierSolution
@18:30:56.44 delay 2 seconds
@18:30:58.47 AS.reconfigure ASconfig
@18:30:58.52 AS.seek 1ml
@18:31:27.54 delay 1 second
@18:31:28.56 AFV.select :holdingCoil
@18:31:28.66 delay 1 second
@18:31:29.68 ATV.dial :waste
@18:31:31.46 AS.reconfigure ASnorm
@18:31:31.52 AS.seek 0ml
@18:32:22.22 AFV.select :carrierSolution
@18:32:22.33 delay 2 seconds
@18:32:24.36 AS.reconfigure ASconfig
@18:32:24.41 AS.seek 0.5ml
@18:32:53.49 delay 1 second
@18:32:54.51 AFV.select :holdingCoil
@18:32:54.61 Building Slug
@18:32:54.62 ATV.dial :ARV
@18:32:56.47 delay 2 seconds
@18:32:58.49 ARV.dial :air1
@18:32:58.91 delay 2 seconds
@18:33:00.98 AS.reconfigure ASslow2
@18:33:01.04 AS.seek 0.506ml
@18:33:07.57 delay 2 seconds
@18:33:09.59 ARV.dial :MM1
@18:33:10.22 delay 2 seconds
@18:33:12.24 Adding MM1
@18:33:12.32 AS.seek 0.524ml
@18:33:30.91 delay 25 seconds
@18:33:55.93 ARV.dial :PP1
@18:33:56.33 Adding PP1
@18:33:56.34 delay 2 seconds
@18:33:58.42 AS.seek 0.53ml
@18:34:05.01 delay 10 seconds
@18:34:15.04 ARV.dial :air1
@18:34:15.46 adding ARV air
@18:34:15.47 delay 2 seconds
@18:34:17.55 AS.seek 0.536ml
@18:34:23.94 delay 5 seconds
@18:34:28.96 ARV.dial :carrier
@18:34:29.80 delay 7 seconds
@18:34:36.87 AS.seek 0.594ml
@18:35:35.74 delay 5 seconds
@18:35:40.76 ATV.dial :ASV
@18:35:41.19 delay 2 seconds
@18:35:43.26 AS.seek 0.6ml
@18:35:49.77 delay 4 seconds
@18:35:53.79 ATV.dial ARV
@18:35:54.38 delay 2 seconds
@18:35:56.40 ARV.dial :carrier
@18:35:56.41 delay 2 seconds
@18:35:58.49 AS.seek 0.65ml
@18:36:49.10 delay 4 seconds
@18:36:53.12 ATV.dial PCR
@18:36:53.77 ASV.dial :waste1
@18:36:53.78 ARV.dial :air1
@18:36:54.20 Positioning Slug by 0.324ml
@18:36:54.29 AS.seek 0.326ml
@18:42:20.66 delay 5 seconds
@18:42:25.68 ATV.dial ARV
@18:42:26.50 ASV.dial :PCRlock
@18:42:26.91 delay 5 seconds
@18:42:31.93 Running AOPP1 Profile60CIQ1 thermocycling
@18:42:31.94 <PCR> PCR.program Profile60CIQ1
@18:42:32.06 Profile60CIQ1 should complete in < 1:56:15
@18:42:32.08 PCR.run 2:30:18.75
@20:22:07.12 PCR.off
@20:22:07.68 <MFBPCR> Running AOPP1 MeltProfile60 thermocycling
@20:22:07.69 <PCR> PCR.program MeltProfile60
@20:22:07.79 MeltProfile60 should complete in < 11:55
@20:22:07.80 PCR.run 19:53.75
@20:30:04.08 PCR.off
@20:30:05.00 <MFBPCR> PCR for AOPP1 completed -- closed /var/log/esp/AOPP121apr2318h.pcr
@20:30:05.08 Cleaning PCR
@20:30:05.09 AFV.select :carrierSolution
@20:30:05.21 delay 2 seconds
@20:30:07.24 AS.reconfigure ASconfig
@20:30:07.29 AS.seek 0.5ml
@20:30:17.60 delay 1 second
@20:30:18.62 AFV.select :holdingCoil
@20:30:18.72 ASV.dial :waste1
@20:30:19.13 ATV.dial :air3
@20:30:20.47 AS.reconfigure ASslow1
@20:30:20.53 AS.seek 0.51ml
@20:30:22.82 delay 2 seconds
@20:30:24.84 ATV.dial :bleach
@20:30:25.47 AS.seek 0.81ml
@20:31:26.08 delay 3 seconds
@20:31:29.10 ATV.dial :air3
@20:31:29.69 delay 3 seconds
@20:31:32.76 AS.seek 0.835ml
@20:31:38.09 delay 3 seconds
@20:31:41.11 ATV.dial :bleach
@20:31:41.76 AS.seek 0.82ml
@20:31:45.03 delay 3 seconds
@20:31:48.05 ARV.dial :waste
@20:31:48.69 delay 3 seconds
@20:31:51.71 ATV.dial :ARV
@20:31:52.63 AS.reconfigure ASconfig
@20:31:52.69 AS.seek 0.47ml
@20:32:13.10 AFV.select :carrierSolution
@20:32:13.21 delay 2 seconds
@20:32:15.24 AS.seek 1ml
@20:32:46.02 delay 1 second
@20:32:47.04 AFV.select :holdingCoil
@20:32:47.14 delay 1 second
@20:32:48.17 AS.reconfigure ASfast
@20:32:48.22 AS.seek 0ml
@20:33:15.58 AFV.select :carrierSolution
@20:33:15.69 delay 2 seconds
@20:33:17.71 AS.reconfigure ASconfig
@20:33:17.77 AS.seek 1ml
@20:34:15.53 delay 1 second
@20:34:16.55 AFV.select :holdingCoil
@20:34:16.65 delay 1 second
@20:34:17.68 AS.reconfigure ASfast
@20:34:17.73 AS.seek 0ml
@20:34:45.08 AFV.select :carrierSolution
@20:34:45.18 delay 2 seconds
@20:34:47.20 AS.reconfigure ASconfig
@20:34:47.26 AS.seek 1ml
@20:35:45.05 delay 1 second
@20:35:46.07 AFV.select :holdingCoil
@20:35:46.17 delay 1 second
@20:35:47.20 AS.reconfigure ASfast
@20:35:47.25 AS.seek 0ml
@20:36:14.60 ARV.dial between carrier and air1
@20:36:15.13 ATV.dial :air3
@20:36:16.47 AS.reconfigure ASslow1
@20:36:16.53 AS.seek 0.01ml
@20:36:19.02 delay 2 seconds
@20:36:21.04 ATV.dial :bleach
@20:36:21.68 AS.seek 0.81ml
@20:39:02.95 delay 3 seconds
@20:39:05.97 ATV.dial :air3
@20:39:06.56 delay 3 seconds
@20:39:10.04 AS.seek 0.835ml
@20:39:15.39 delay 3 seconds
@20:39:18.41 ATV.dial :bleach
@20:39:19.06 AS.seek 0.82ml
@20:39:22.44 delay 3 seconds
@20:39:25.46 ATV.dial PCR
@20:39:25.88 AS.reconfigure ASconfig
@20:39:25.94 AS.seek 0ml
@20:40:13.39 PCR.program :bleachProfile
@20:40:13.46 PCR program should complete in < 2:45
@20:40:13.47 PCR.run 10:00
@20:43:09.26 PCR.off
@20:43:09.30 AFV.select :carrierSolution
@20:43:09.41 delay 2 seconds
@20:43:11.44 AS.seek 1ml
@20:44:09.17 delay 1 second
@20:44:10.19 AFV.select :holdingCoil
@20:44:10.29 delay 1 second
@20:44:11.32 AS.reconfigure ASfast
@20:44:11.37 AS.seek 0ml
@20:44:38.70 delay 3 seconds
@20:44:41.72 AFV.select :carrierSolution
@20:44:41.82 delay 2 seconds
@20:44:43.85 AS.reconfigure ASconfig
@20:44:43.91 AS.seek 1ml
@20:45:41.66 delay 1 second
@20:45:42.68 AFV.select :holdingCoil
@20:45:42.78 delay 1 second
@20:45:43.81 AS.reconfigure ASfast
@20:45:43.86 AS.seek 0ml
@20:46:11.20 delay 3 seconds
@20:46:14.22 AFV.select :carrierSolution
@20:46:14.32 delay 2 seconds
@20:46:16.34 AS.reconfigure ASconfig
@20:46:16.41 AS.seek 1ml
@20:47:14.16 delay 1 second
@20:47:15.18 AFV.select :holdingCoil
@20:47:15.28 delay 1 second
@20:47:16.31 AS.reconfigure ASfast
@20:47:16.36 AS.seek 0ml
@20:47:43.70 delay 3 seconds
@20:47:46.72 AFV.select :carrierSolution
@20:47:46.82 delay 2 seconds
@20:47:48.84 AS.reconfigure ASconfig
@20:47:48.91 AS.seek 1ml
@20:48:46.69 delay 1 second
@20:48:47.71 AFV.select :holdingCoil
@20:48:47.81 delay 1 second
@20:48:48.84 AS.reconfigure ASfast
@20:48:48.89 AS.seek 0ml
@20:49:16.24 delay 3 seconds
@20:49:19.26 AFV.select :carrierSolution
@20:49:19.36 delay 2 seconds
@20:49:21.38 AS.reconfigure ASconfig
@20:49:21.45 AS.seek 1ml
@20:50:19.28 delay 1 second
@20:50:20.30 AFV.select :holdingCoil
@20:50:20.40 delay 1 second
@20:50:21.43 AS.reconfigure ASnorm
@20:50:21.48 AS.seek 0ml
@20:51:12.21 Setting up PCR for AARPP4 -- writing /var/log/esp/AARPP421apr2320h.pcr
@20:51:12.23 Building PCR Slug from ASV with MM2 mix, PP4 primer
@20:51:12.25 Priming with carrier
@20:51:12.27 ATV.dial ARV
@20:51:12.94 ARV.dial :carrier
@20:51:13.30 AS.seek 0.5ml
@20:51:38.84 AFV.select :carrierSolution
@20:51:38.95 delay 2 seconds
@20:51:40.98 AS.reconfigure ASconfig
@20:51:41.44 AS.seek 1ml
@20:52:10.47 delay 1 second
@20:52:11.49 AFV.select :holdingCoil
@20:52:11.59 delay 1 second
@20:52:12.61 ATV.dial :waste
@20:52:14.39 AS.reconfigure ASnorm
@20:52:14.46 AS.seek 0ml
@20:53:05.16 AFV.select :carrierSolution
@20:53:05.27 delay 2 seconds
@20:53:07.30 AS.reconfigure ASconfig
@20:53:07.35 AS.seek 0.5ml
@20:53:36.38 delay 1 second
@20:53:37.40 AFV.select :holdingCoil
@20:53:37.50 Building Slug
@20:53:37.51 ATV.dial :ARV
@20:53:39.25 delay 2 seconds
@20:53:41.27 ARV.dial :air1
@20:53:41.69 delay 2 seconds
@20:53:43.76 AS.reconfigure ASslow2
@20:53:43.82 AS.seek 0.506ml
@20:53:50.41 delay 2 seconds
@20:53:52.43 ARV.dial :MM2
@20:53:54.02 delay 2 seconds
@20:53:56.04 Adding MM2
@20:53:56.12 AS.seek 0.524ml
@20:54:14.49 delay 25 seconds
@20:54:39.51 ARV.dial :PP4
@20:54:39.94 Adding PP4
@20:54:39.95 delay 2 seconds
@20:54:42.03 AS.seek 0.53ml
@20:54:49.17 delay 10 seconds
@20:54:59.20 ARV.dial :air3
@20:54:59.63 adding ARV air
@20:54:59.64 delay 2 seconds
@20:55:01.72 AS.seek 0.536ml
@20:55:08.08 delay 5 seconds
@20:55:13.10 ARV.dial :carrier
@20:55:14.47 delay 7 seconds
@20:55:21.55 AS.seek 0.594ml
@20:56:20.19 delay 5 seconds
@20:56:25.21 ATV.dial :ASV
@20:56:25.64 delay 2 seconds
@20:56:27.71 AS.seek 0.6ml
@20:56:34.22 delay 4 seconds
@20:56:38.24 ATV.dial ARV
@20:56:38.83 delay 2 seconds
@20:56:40.85 ARV.dial :carrier
@20:56:40.86 delay 2 seconds
@20:56:42.94 AS.seek 0.65ml
@20:57:33.61 delay 4 seconds
@20:57:37.63 ATV.dial PCR
@20:57:38.28 ASV.dial :waste1
@20:57:38.29 ARV.dial :air1
@20:57:38.71 Positioning Slug by 0.324ml
@20:57:38.80 AS.seek 0.326ml
@21:03:04.75 delay 5 seconds
@21:03:09.77 ATV.dial ARV
@21:03:10.59 ASV.dial :PCRlock
@21:03:10.99 delay 5 seconds
@21:03:16.01 Running AARPP4 Profile58CIQ2 thermocycling
@21:03:16.02 <PCR> PCR.program Profile58CIQ2
@21:03:16.14 Profile58CIQ2 should complete in < 1:36:15
@21:03:16.15 PCR.run 2:05:18.75
@22:24:24.72 PCR.off
@22:24:25.29 <MFBPCR> Running AARPP4 MeltProfile58 thermocycling
@22:24:25.30 <PCR> PCR.program MeltProfile58
@22:24:25.40 MeltProfile58 should complete in < 12:33
@22:24:25.41 PCR.run 20:41.25
@22:32:22.47 PCR.off
@22:32:23.44 <MFBPCR> PCR for AARPP4 completed -- closed /var/log/esp/AARPP421apr2320h.pcr
@22:32:23.51 Cleaning PCR
@22:32:23.52 AFV.select :carrierSolution
@22:32:23.64 delay 2 seconds
@22:32:25.67 AS.reconfigure ASconfig
@22:32:25.72 AS.seek 0.5ml
@22:32:36.06 delay 1 second
@22:32:37.08 AFV.select :holdingCoil
@22:32:37.18 ASV.dial :waste1
@22:32:37.58 ATV.dial :air3
@22:32:38.95 AS.reconfigure ASslow1
@22:32:39.00 AS.seek 0.51ml
@22:32:41.31 delay 2 seconds
@22:32:43.33 ATV.dial :bleach
@22:32:44.00 AS.seek 0.81ml
@22:33:44.59 delay 3 seconds
@22:33:47.61 ATV.dial :air3
@22:33:48.59 delay 3 seconds
@22:33:51.66 AS.seek 0.835ml
@22:33:56.96 delay 3 seconds
@22:33:59.98 ATV.dial :bleach
@22:34:00.64 AS.seek 0.82ml
@22:34:03.95 delay 3 seconds
@22:34:06.97 ARV.dial :waste
@22:34:07.61 delay 3 seconds
@22:34:10.63 ATV.dial :ARV
@22:34:11.55 AS.reconfigure ASconfig
@22:34:11.62 AS.seek 0.47ml
@22:34:32.01 AFV.select :carrierSolution
@22:34:32.12 delay 2 seconds
@22:34:34.15 AS.seek 1ml
@22:35:04.92 delay 1 second
@22:35:05.94 AFV.select :holdingCoil
@22:35:06.04 delay 1 second
@22:35:07.07 AS.reconfigure ASfast
@22:35:07.12 AS.seek 0ml
@22:35:34.48 AFV.select :carrierSolution
@22:35:34.59 delay 2 seconds
@22:35:36.62 AS.reconfigure ASconfig
@22:35:36.67 AS.seek 1ml
@22:36:34.47 delay 1 second
@22:36:35.49 AFV.select :holdingCoil
@22:36:35.59 delay 1 second
@22:36:36.62 AS.reconfigure ASfast
@22:36:36.67 AS.seek 0ml
@22:37:04.01 AFV.select :carrierSolution
@22:37:04.12 delay 2 seconds
@22:37:06.15 AS.reconfigure ASconfig
@22:37:06.20 AS.seek 1ml
@22:38:03.97 delay 1 second
@22:38:04.99 AFV.select :holdingCoil
@22:38:05.09 delay 1 second
@22:38:06.12 AS.reconfigure ASfast
@22:38:06.17 AS.seek 0ml
@22:38:33.53 ARV.dial between carrier and air1
@22:38:34.06 ATV.dial :air3
@22:38:35.41 AS.reconfigure ASslow1
@22:38:35.47 AS.seek 0.01ml
@22:38:37.81 delay 2 seconds
@22:38:39.83 ATV.dial :bleach
@22:38:40.48 AS.seek 0.81ml
@22:41:21.69 delay 3 seconds
@22:41:24.71 ATV.dial :air3
@22:41:25.30 delay 3 seconds
@22:41:28.37 AS.seek 0.835ml
@22:41:33.66 delay 3 seconds
@22:41:36.68 ATV.dial :bleach
@22:41:37.31 AS.seek 0.82ml
@22:41:40.64 delay 3 seconds
@22:41:43.66 ATV.dial PCR
@22:41:44.09 AS.reconfigure ASconfig
@22:41:44.15 AS.seek 0ml
@22:42:31.55 PCR.program :bleachProfile
@22:42:31.61 PCR program should complete in < 2:45
@22:42:31.62 PCR.run 10:00
@22:45:27.42 PCR.off
@22:45:27.87 AFV.select :carrierSolution
@22:45:27.97 delay 2 seconds
@22:45:30.00 AS.seek 1ml
@22:46:27.75 delay 1 second
@22:46:28.77 AFV.select :holdingCoil
@22:46:28.87 delay 1 second
@22:46:29.90 AS.reconfigure ASfast
@22:46:29.95 AS.seek 0ml
@22:46:57.28 delay 3 seconds
@22:47:00.30 AFV.select :carrierSolution
@22:47:00.40 delay 2 seconds
@22:47:02.43 AS.reconfigure ASconfig
@22:47:02.49 AS.seek 1ml
@22:48:00.28 delay 1 second
@22:48:01.30 AFV.select :holdingCoil
@22:48:01.40 delay 1 second
@22:48:02.43 AS.reconfigure ASfast
@22:48:02.48 AS.seek 0ml
@22:48:29.81 delay 3 seconds
@22:48:32.83 AFV.select :carrierSolution
@22:48:32.93 delay 2 seconds
@22:48:34.96 AS.reconfigure ASconfig
@22:48:35.02 AS.seek 1ml
@22:49:32.78 delay 1 second
@22:49:33.80 AFV.select :holdingCoil
@22:49:33.90 delay 1 second
@22:49:34.93 AS.reconfigure ASfast
@22:49:34.98 AS.seek 0ml
@22:50:02.34 delay 3 seconds
@22:50:05.36 AFV.select :carrierSolution
@22:50:05.46 delay 2 seconds
@22:50:07.49 AS.reconfigure ASconfig
@22:50:07.54 AS.seek 1ml
@22:51:05.33 delay 1 second
@22:51:06.35 AFV.select :holdingCoil
@22:51:06.45 delay 1 second
@22:51:07.48 AS.reconfigure ASfast
@22:51:07.53 AS.seek 0ml
@22:51:34.87 delay 3 seconds
@22:51:37.89 AFV.select :carrierSolution
@22:51:37.99 delay 2 seconds
@22:51:40.02 AS.reconfigure ASconfig
@22:51:40.08 AS.seek 1ml
@22:52:37.83 delay 1 second
@22:52:38.85 AFV.select :holdingCoil
@22:52:38.95 delay 1 second
@22:52:39.98 AS.reconfigure ASnorm
@22:52:40.03 AS.seek 0ml
@22:53:30.75 Setting up PCR for AALPP3 -- writing /var/log/esp/AALPP321apr2322h.pcr
@22:53:30.77 Building PCR Slug from ASV with MM2 mix, PP3 primer
@22:53:30.79 Priming with carrier
@22:53:30.81 ATV.dial ARV
@22:53:31.45 ARV.dial :carrier
@22:53:31.81 AS.seek 0.5ml
@22:53:57.47 AFV.select :carrierSolution
@22:53:57.59 delay 2 seconds
@22:53:59.62 AS.reconfigure ASconfig
@22:53:59.67 AS.seek 1ml
@22:54:28.69 delay 1 second
@22:54:29.71 AFV.select :holdingCoil
@22:54:29.81 delay 1 second
@22:54:30.83 ATV.dial :waste
@22:54:32.61 AS.reconfigure ASnorm
@22:54:32.67 AS.seek 0ml
@22:55:23.37 AFV.select :carrierSolution
@22:55:23.49 delay 2 seconds
@22:55:25.52 AS.reconfigure ASconfig
@22:55:25.57 AS.seek 0.5ml
@22:55:54.61 delay 1 second
@22:55:55.63 AFV.select :holdingCoil
@22:55:55.73 Building Slug
@22:55:55.74 ATV.dial :ARV
@22:55:57.50 delay 2 seconds
@22:55:59.52 ARV.dial :air1
@22:55:59.93 delay 2 seconds
@22:56:02.00 AS.reconfigure ASslow2
@22:56:02.06 AS.seek 0.506ml
@22:56:08.26 delay 2 seconds
@22:56:10.28 ARV.dial :MM2
@22:56:11.87 delay 2 seconds
@22:56:13.89 Adding MM2
@22:56:13.96 AS.seek 0.524ml
@22:56:32.19 delay 25 seconds
@22:56:57.21 ARV.dial :PP3
@22:56:57.61 Adding PP3
@22:56:57.62 delay 2 seconds
@22:56:59.70 AS.seek 0.53ml
@22:57:06.19 delay 10 seconds
@22:57:16.22 ARV.dial :air2
@22:57:16.66 adding ARV air
@22:57:16.67 delay 2 seconds
@22:57:18.75 AS.seek 0.536ml
@22:57:25.45 delay 5 seconds
@22:57:30.47 ARV.dial :carrier
@22:57:31.81 delay 7 seconds
@22:57:38.89 AS.seek 0.594ml
@22:58:37.45 delay 5 seconds
@22:58:42.47 ATV.dial :ASV
@22:58:42.87 delay 2 seconds
@22:58:44.94 AS.seek 0.6ml
@22:58:51.45 delay 4 seconds
@22:58:55.47 ATV.dial ARV
@22:58:56.06 delay 2 seconds
@22:58:58.08 ARV.dial :carrier
@22:58:58.09 delay 2 seconds
@22:59:00.17 AS.seek 0.65ml
@22:59:50.67 delay 4 seconds
@22:59:54.69 ATV.dial PCR
@22:59:55.33 ASV.dial :waste1
@22:59:55.34 ARV.dial :air1
@22:59:55.76 Positioning Slug by 0.324ml
@22:59:55.85 AS.seek 0.326ml
@23:05:22.03 delay 5 seconds
@23:05:27.05 ATV.dial ARV
@23:05:27.87 ASV.dial :PCRlock
@23:05:28.28 delay 5 seconds
@23:05:33.30 Running AALPP3 Profile58CIQ2 thermocycling
@23:05:33.32 <PCR> PCR.program Profile58CIQ2
@23:05:33.44 Profile58CIQ2 should complete in < 1:36:15
@23:05:33.45 PCR.run 2:05:18.75
@00:26:46.54CEST24-Apr-21 PCR.off
@00:26:47.09 <MFBPCR> Running AALPP3 MeltProfile58 thermocycling
@00:26:47.11 <PCR> PCR.program MeltProfile58
@00:26:47.21 MeltProfile58 should complete in < 12:33
@00:26:47.22 PCR.run 20:41.25
@00:34:47.44 PCR.off
@00:34:48.42 <MFBPCR> PCR for AALPP3 completed -- closed /var/log/esp/AALPP321apr2322h.pcr
@00:34:48.49 Cleaning PCR
@00:34:48.50 AFV.select :carrierSolution
@00:34:48.62 delay 2 seconds
@00:34:50.66 AS.reconfigure ASconfig
@00:34:50.76 AS.seek 0.5ml
@00:35:01.17 delay 1 second
@00:35:02.19 AFV.select :holdingCoil
@00:35:02.32 ASV.dial :waste1
@00:35:02.75 ATV.dial :air3
@00:35:04.20 AS.reconfigure ASslow1
@00:35:04.32 AS.seek 0.51ml
@00:35:06.64 delay 2 seconds
@00:35:08.67 ATV.dial :bleach
@00:35:09.32 AS.seek 0.81ml
@00:36:09.92 delay 3 seconds
@00:36:12.94 ATV.dial :air3
@00:36:13.53 delay 3 seconds
@00:36:16.60 AS.seek 0.835ml
@00:36:21.91 delay 3 seconds
@00:36:24.93 ATV.dial :bleach
@00:36:25.57 AS.seek 0.82ml
@00:36:28.87 delay 3 seconds
@00:36:31.89 ARV.dial :waste
@00:36:32.53 delay 3 seconds
@00:36:35.55 ATV.dial :ARV
@00:36:36.47 AS.reconfigure ASconfig
@00:36:36.53 AS.seek 0.47ml
@00:36:56.95 AFV.select :carrierSolution
@00:36:57.06 delay 2 seconds
@00:36:59.09 AS.seek 1ml
@00:37:29.86 delay 1 second
@00:37:30.88 AFV.select :holdingCoil
@00:37:30.98 delay 1 second
@00:37:32.01 AS.reconfigure ASfast
@00:37:32.06 AS.seek 0ml
@00:37:59.40 AFV.select :carrierSolution
@00:37:59.51 delay 2 seconds
@00:38:01.53 AS.reconfigure ASconfig
@00:38:01.59 AS.seek 1ml
@00:38:59.34 delay 1 second
@00:39:00.36 AFV.select :holdingCoil
@00:39:00.46 delay 1 second
@00:39:01.49 AS.reconfigure ASfast
@00:39:01.54 AS.seek 0ml
@00:39:28.89 AFV.select :carrierSolution
@00:39:29.00 delay 2 seconds
@00:39:31.02 AS.reconfigure ASconfig
@00:39:31.08 AS.seek 1ml
@00:40:28.84 delay 1 second
@00:40:29.86 AFV.select :holdingCoil
@00:40:29.96 delay 1 second
@00:40:30.99 AS.reconfigure ASfast
@00:40:31.04 AS.seek 0ml
@00:40:58.39 ARV.dial between carrier and air1
@00:40:58.92 ATV.dial :air3
@00:41:00.27 AS.reconfigure ASslow1
@00:41:00.33 AS.seek 0.01ml
@00:41:02.75 delay 2 seconds
@00:41:04.77 ATV.dial :bleach
@00:41:05.42 AS.seek 0.81ml
@00:43:46.61 delay 3 seconds
@00:43:49.63 ATV.dial :air3
@00:43:50.22 delay 3 seconds
@00:43:53.29 AS.seek 0.835ml
@00:43:58.59 delay 3 seconds
@00:44:01.61 ATV.dial :bleach
@00:44:02.25 AS.seek 0.82ml
@00:44:05.67 delay 3 seconds
@00:44:08.69 ATV.dial PCR
@00:44:09.11 AS.reconfigure ASconfig
@00:44:09.17 AS.seek 0ml
@00:44:56.61 PCR.program :bleachProfile
@00:44:56.68 PCR program should complete in < 2:45
@00:44:56.69 PCR.run 10:00
@00:47:52.90 PCR.off
@00:47:52.94 AFV.select :carrierSolution
@00:47:53.05 delay 2 seconds
@00:47:55.08 AS.seek 1ml
@00:48:52.87 delay 1 second
@00:48:53.89 AFV.select :holdingCoil
@00:48:53.99 delay 1 second
@00:48:55.02 AS.reconfigure ASfast
@00:48:55.07 AS.seek 0ml
@00:49:22.42 delay 3 seconds
@00:49:25.44 AFV.select :carrierSolution
@00:49:25.54 delay 2 seconds
@00:49:27.56 AS.reconfigure ASconfig
@00:49:27.62 AS.seek 1ml
@00:50:25.42 delay 1 second
@00:50:26.44 AFV.select :holdingCoil
@00:50:26.54 delay 1 second
@00:50:27.57 AS.reconfigure ASfast
@00:50:27.62 AS.seek 0ml
@00:50:54.95 delay 3 seconds
@00:50:57.97 AFV.select :carrierSolution
@00:50:58.07 delay 2 seconds
@00:51:00.09 AS.reconfigure ASconfig
@00:51:00.20 AS.seek 1ml
@00:51:57.95 delay 1 second
@00:51:58.97 AFV.select :holdingCoil
@00:51:59.07 delay 1 second
@00:52:00.10 AS.reconfigure ASfast
@00:52:00.15 AS.seek 0ml
@00:52:27.50 delay 3 seconds
@00:52:30.52 AFV.select :carrierSolution
@00:52:30.62 delay 2 seconds
@00:52:32.65 AS.reconfigure ASconfig
@00:52:32.71 AS.seek 1ml
@00:53:30.45 delay 1 second
@00:53:31.47 AFV.select :holdingCoil
@00:53:31.57 delay 1 second
@00:53:32.60 AS.reconfigure ASfast
@00:53:32.65 AS.seek 0ml
@00:54:00.00 delay 3 seconds
@00:54:03.02 AFV.select :carrierSolution
@00:54:03.12 delay 2 seconds
@00:54:05.14 AS.reconfigure ASconfig
@00:54:05.21 AS.seek 1ml
@00:55:02.95 delay 1 second
@00:55:03.97 AFV.select :holdingCoil
@00:55:04.07 delay 1 second
@00:55:05.10 AS.reconfigure ASnorm
@00:55:05.15 AS.seek 0ml
@00:55:55.86 Setting up PCR for ACOPP5 -- writing /var/log/esp/ACOPP521apr2400h.pcr
@00:55:55.88 Building PCR Slug from ASV with MM3 mix, PP5 primer
@00:55:55.90 Priming with carrier
@00:55:55.92 ATV.dial ARV
@00:55:56.57 ARV.dial :carrier
@00:55:56.92 AS.seek 0.5ml
@00:56:22.54 AFV.select :carrierSolution
@00:56:22.66 delay 2 seconds
@00:56:24.69 AS.reconfigure ASconfig
@00:56:24.74 AS.seek 1ml
@00:56:53.76 delay 1 second
@00:56:54.78 AFV.select :holdingCoil
@00:56:54.88 delay 1 second
@00:56:55.90 ATV.dial :waste
@00:56:57.68 AS.reconfigure ASnorm
@00:56:57.74 AS.seek 0ml
@00:57:48.43 AFV.select :carrierSolution
@00:57:48.54 delay 2 seconds
@00:57:50.57 AS.reconfigure ASconfig
@00:57:50.63 AS.seek 0.5ml
@00:58:19.72 delay 1 second
@00:58:20.74 AFV.select :holdingCoil
@00:58:20.84 Building Slug
@00:58:20.85 ATV.dial :ARV
@00:58:22.61 delay 2 seconds
@00:58:24.63 ARV.dial :air1
@00:58:25.04 delay 2 seconds
@00:58:27.11 AS.reconfigure ASslow2
@00:58:27.17 AS.seek 0.506ml
@00:58:34.42 delay 2 seconds
@00:58:36.45 ARV.dial :MM3
@00:58:37.57 delay 2 seconds
@00:58:39.59 Adding MM3
@00:58:39.67 AS.seek 0.524ml
@00:58:58.11 delay 25 seconds
@00:59:23.13 ARV.dial :PP5
@00:59:23.56 Adding PP5
@00:59:23.57 delay 2 seconds
@00:59:25.65 AS.seek 0.53ml
@00:59:32.67 delay 10 seconds
@00:59:42.70 ARV.dial :air3
@00:59:43.11 adding ARV air
@00:59:43.12 delay 2 seconds
@00:59:45.20 AS.seek 0.536ml
@00:59:51.79 delay 5 seconds
@00:59:56.81 ARV.dial :carrier
@00:59:58.15 delay 7 seconds
@01:00:05.23 AS.seek 0.594ml
@01:01:03.95 delay 5 seconds
@01:01:08.97 ATV.dial :ASV
@01:01:09.39 delay 2 seconds
@01:01:11.46 AS.seek 0.6ml
@01:01:18.06 delay 4 seconds
@01:01:22.08 ATV.dial ARV
@01:01:22.76 delay 2 seconds
@01:01:24.78 ARV.dial :carrier
@01:01:24.79 delay 2 seconds
@01:01:26.87 AS.seek 0.65ml
@01:02:17.72 delay 4 seconds
@01:02:21.74 ATV.dial PCR
@01:02:22.40 ASV.dial :waste1
@01:02:22.41 ARV.dial :air1
@01:02:22.85 Positioning Slug by 0.324ml
@01:02:22.94 AS.seek 0.326ml
@01:07:49.12 delay 5 seconds
@01:07:54.14 ATV.dial ARV
@01:07:54.95 ASV.dial :PCRlock
@01:07:55.36 delay 5 seconds
@01:08:00.38 Running ACOPP5 ProfileIPc thermocycling
@01:08:00.39 <PCR> PCR.program ProfileIPc
@01:08:00.51 ProfileIPc should complete in < 1:26:35
@01:08:00.52 PCR.run 1:53:13.75
@02:16:56.64 PCR.off
@02:16:57.20 <MFBPCR> PCR for ACOPP5 completed -- closed /var/log/esp/ACOPP521apr2400h.pcr
@02:16:57.27 Cleaning PCR
@02:16:57.28 AFV.select :carrierSolution
@02:16:57.40 delay 2 seconds
@02:16:59.43 AS.reconfigure ASconfig
@02:16:59.48 AS.seek 0.5ml
@02:17:09.78 delay 1 second
@02:17:10.80 AFV.select :holdingCoil
@02:17:10.90 ASV.dial :waste1
@02:17:11.31 ATV.dial :air3
@02:17:12.67 AS.reconfigure ASslow1
@02:17:12.73 AS.seek 0.51ml
@02:17:15.04 delay 2 seconds
@02:17:17.06 ATV.dial :bleach
@02:17:17.70 AS.seek 0.81ml
@02:18:18.28 delay 3 seconds
@02:18:21.30 ATV.dial :air3
@02:18:21.89 delay 3 seconds
@02:18:24.96 AS.seek 0.835ml
@02:18:30.26 delay 3 seconds
@02:18:33.28 ATV.dial :bleach
@02:18:33.93 AS.seek 0.82ml
@02:18:37.23 delay 3 seconds
@02:18:40.25 ARV.dial :waste
@02:18:40.89 delay 3 seconds
@02:18:43.91 ATV.dial :ARV
@02:18:44.94 AS.reconfigure ASconfig
@02:18:45.00 AS.seek 0.47ml
@02:19:05.40 AFV.select :carrierSolution
@02:19:05.51 delay 2 seconds
@02:19:07.54 AS.seek 1ml
@02:19:38.26 delay 1 second
@02:19:39.28 AFV.select :holdingCoil
@02:19:39.38 delay 1 second
@02:19:40.41 AS.reconfigure ASfast
@02:19:40.46 AS.seek 0ml
@02:20:07.79 AFV.select :carrierSolution
@02:20:07.90 delay 2 seconds
@02:20:09.92 AS.reconfigure ASconfig
@02:20:09.98 AS.seek 1ml
@02:21:07.71 delay 1 second
@02:21:08.73 AFV.select :holdingCoil
@02:21:08.83 delay 1 second
@02:21:09.86 AS.reconfigure ASfast
@02:21:09.91 AS.seek 0ml
@02:21:37.23 AFV.select :carrierSolution
@02:21:37.34 delay 2 seconds
@02:21:39.36 AS.reconfigure ASconfig
@02:21:39.42 AS.seek 1ml
@02:22:37.17 delay 1 second
@02:22:38.19 AFV.select :holdingCoil
@02:22:38.29 delay 1 second
@02:22:39.32 AS.reconfigure ASfast
@02:22:39.37 AS.seek 0ml
@02:23:06.71 ARV.dial between carrier and air1
@02:23:07.25 ATV.dial :air3
@02:23:08.59 AS.reconfigure ASslow1
@02:23:08.65 AS.seek 0.01ml
@02:23:10.92 delay 2 seconds
@02:23:12.94 ATV.dial :bleach
@02:23:13.59 AS.seek 0.81ml
@02:25:54.82 delay 3 seconds
@02:25:57.84 ATV.dial :air3
@02:25:58.43 delay 3 seconds
@02:26:01.50 AS.seek 0.835ml
@02:26:06.90 delay 3 seconds
@02:26:09.92 ATV.dial :bleach
@02:26:10.57 AS.seek 0.82ml
@02:26:13.98 delay 3 seconds
@02:26:17.41 ATV.dial PCR
@02:26:17.82 AS.reconfigure ASconfig
@02:26:17.88 AS.seek 0ml
@02:27:05.28 PCR.program :bleachProfile
@02:27:05.34 PCR program should complete in < 2:45
@02:27:05.35 PCR.run 10:00
@02:30:01.16 PCR.off
@02:30:01.20 AFV.select :carrierSolution
@02:30:01.31 delay 2 seconds
@02:30:03.34 AS.seek 1ml
@02:31:01.14 delay 1 second
@02:31:02.16 AFV.select :holdingCoil
@02:31:02.26 delay 1 second
@02:31:03.29 AS.reconfigure ASfast
@02:31:03.34 AS.seek 0ml
@02:31:30.67 delay 3 seconds
@02:31:33.69 AFV.select :carrierSolution
@02:31:33.79 delay 2 seconds
@02:31:35.81 AS.reconfigure ASconfig
@02:31:35.88 AS.seek 1ml
@02:32:33.60 delay 1 second
@02:32:34.62 AFV.select :holdingCoil
@02:32:34.72 delay 1 second
@02:32:35.75 AS.reconfigure ASfast
@02:32:35.80 AS.seek 0ml
@02:33:03.14 delay 3 seconds
@02:33:06.16 AFV.select :carrierSolution
@02:33:06.26 delay 2 seconds
@02:33:08.28 AS.reconfigure ASconfig
@02:33:08.35 AS.seek 1ml
@02:34:06.14 delay 1 second
@02:34:07.16 AFV.select :holdingCoil
@02:34:07.26 delay 1 second
@02:34:08.29 AS.reconfigure ASfast
@02:34:08.34 AS.seek 0ml
@02:34:35.68 delay 3 seconds
@02:34:38.70 AFV.select :carrierSolution
@02:34:38.80 delay 2 seconds
@02:34:40.82 AS.reconfigure ASconfig
@02:34:40.88 AS.seek 1ml
@02:35:38.65 delay 1 second
@02:35:39.67 AFV.select :holdingCoil
@02:35:39.77 delay 1 second
@02:35:40.80 AS.reconfigure ASfast
@02:35:40.85 AS.seek 0ml
@02:36:08.17 delay 3 seconds
@02:36:11.19 AFV.select :carrierSolution
@02:36:11.29 delay 2 seconds
@02:36:13.31 AS.reconfigure ASconfig
@02:36:13.38 AS.seek 1ml
@02:37:11.15 delay 1 second
@02:37:12.17 AFV.select :holdingCoil
@02:37:12.27 delay 1 second
@02:37:13.30 AS.reconfigure ASnorm
@02:37:13.35 AS.seek 0ml
@02:38:04.06 Setting up PCR for AIPc -- writing /var/log/esp/AIPc21apr2402h.pcr
@02:38:04.08 Building PCR Slug from ASV with MM3 mix, PP6 primer
@02:38:04.10 Priming with carrier
@02:38:04.12 ATV.dial ARV
@02:38:04.78 ARV.dial :carrier
@02:38:05.13 AS.seek 0.5ml
@02:38:30.68 AFV.select :carrierSolution
@02:38:30.79 delay 2 seconds
@02:38:32.82 AS.reconfigure ASconfig
@02:38:32.88 AS.seek 1ml
@02:39:01.92 delay 1 second
@02:39:02.94 AFV.select :holdingCoil
@02:39:03.04 delay 1 second
@02:39:04.06 ATV.dial :waste
@02:39:06.25 AS.reconfigure ASnorm
@02:39:06.31 AS.seek 0ml
@02:39:56.99 AFV.select :carrierSolution
@02:39:57.11 delay 2 seconds
@02:39:59.14 AS.reconfigure ASconfig
@02:39:59.19 AS.seek 0.5ml
@02:40:28.23 delay 1 second
@02:40:29.25 AFV.select :holdingCoil
@02:40:29.35 Building Slug
@02:40:29.36 ATV.dial :ARV
@02:40:31.10 delay 2 seconds
@02:40:33.12 ARV.dial :air1
@02:40:33.66 delay 2 seconds
@02:40:35.73 AS.reconfigure ASslow2
@02:40:35.79 AS.seek 0.506ml
@02:40:42.17 delay 2 seconds
@02:40:44.19 ARV.dial :MM3
@02:40:45.29 delay 2 seconds
@02:40:47.31 Adding MM3
@02:40:47.40 AS.seek 0.524ml
@02:41:06.06 delay 25 seconds
@02:41:31.08 ARV.dial :PP6
@02:41:31.48 Adding PP6
@02:41:31.49 delay 2 seconds
@02:41:33.57 AS.seek 0.53ml
@02:41:39.76 delay 10 seconds
@02:41:49.79 ARV.dial :air1
@02:41:50.67 adding ARV air
@02:41:50.68 delay 2 seconds
@02:41:52.76 AS.seek 0.536ml
@02:41:59.00 delay 5 seconds
@02:42:04.02 ARV.dial :carrier
@02:42:04.45 delay 7 seconds
@02:42:11.53 AS.seek 0.594ml
@02:43:10.32 delay 5 seconds
@02:43:15.34 ATV.dial :ASV
@02:43:15.78 delay 2 seconds
@02:43:17.85 AS.seek 0.6ml
@02:43:24.03 delay 4 seconds
@02:43:28.05 ATV.dial ARV
@02:43:28.65 delay 2 seconds
@02:43:30.67 ARV.dial :carrier
@02:43:30.68 delay 2 seconds
@02:43:32.76 AS.seek 0.65ml
@02:44:23.42 delay 4 seconds
@02:44:27.44 ATV.dial PCR
@02:44:28.09 ASV.dial :waste1
@02:44:28.10 ARV.dial :air1
@02:44:28.51 Positioning Slug by 0.324ml
@02:44:28.60 AS.seek 0.326ml
@02:49:54.76 delay 5 seconds
@02:49:59.78 ATV.dial ARV
@02:50:00.59 ASV.dial :PCRlock
@02:50:01.00 delay 5 seconds
@02:50:06.02 Running AIPc ProfileIPc thermocycling
@02:50:06.03 <PCR> PCR.program ProfileIPc
@02:50:06.15 ProfileIPc should complete in < 1:26:35
@02:50:06.16 PCR.run 1:53:13.75
@03:58:58.45 PCR.off
@03:58:59.15 <MFBPCR> PCR for AIPc completed -- closed /var/log/esp/AIPc21apr2402h.pcr
@03:58:59.17 Full MFB Decontamination
@03:58:59.20 Decon extraction column and decon extSrc
@03:58:59.25 SPE.hold 2:46:40,55C
@03:58:59.35 ASV.dial :SPEdelivery
@03:59:00.11 delay 20 seconds
@03:59:20.15 AFV.select :carrierSolution
@03:59:20.25 delay 2 seconds
@03:59:22.28 AS.reconfigure ASconfig
@03:59:22.33 AS.seek 0.5ml
@03:59:32.65 delay 1 second
@03:59:33.67 AFV.select :holdingCoil
@03:59:33.77 ATV.dial :air1
@03:59:35.83 AS.reconfigure ASslow1
@03:59:35.89 AS.seek 0.52ml
@03:59:40.23 ATV.dial :ASV
@03:59:42.18 ASV.dial :SPErecovery
@03:59:42.88 AS.seek 0.62ml
@04:00:03.28 ATV.dial :waste
@04:00:05.48 AS.reconfigure ASnorm
@04:00:05.54 AS.seek 0ml
@04:00:37.12 deconEluateRecoveryLine extSrc
@04:00:37.13 ATV.dial :waste
@04:00:37.16 AS.seek 0ml
@04:00:37.24 ATV.dial :air3
@04:00:38.02 AS.reconfigure ASslow1
@04:00:38.08 AS.seek 0.01ml
@04:00:40.35 delay 2 seconds
@04:00:42.37 ATV.dial :bleach
@04:00:42.88 AS.seek 0.31ml
@04:01:43.62 delay 3 seconds
@04:01:46.64 ATV.dial :air3
@04:01:47.26 delay 3 seconds
@04:01:50.33 AS.seek 0.335ml
@04:01:55.59 delay 3 seconds
@04:01:58.61 ATV.dial :bleach
@04:01:59.29 AS.seek 0.32ml
@04:02:02.53 delay 3 seconds
@04:02:05.55 ATV.dial :extSrc
@04:02:06.23 AS.seek 0ml
@04:03:10.87 AFV.select :carrierSolution
@04:03:10.98 delay 2 seconds
@04:03:13.01 AS.reconfigure ASconfig
@04:03:13.06 AS.seek 0.5ml
@04:03:42.09 delay 1 second
@04:03:43.11 AFV.select :holdingCoil
@04:03:43.21 ATV.dial :extSrc
@04:03:43.23 AS.reconfigure ASslow1
@04:03:43.29 AS.seek 0ml
@04:05:24.26 AFV.select :carrierSolution
@04:05:24.37 delay 2 seconds
@04:05:26.40 AS.reconfigure ASconfig
@04:05:26.87 AS.seek 0.5ml
@04:05:55.95 delay 1 second
@04:05:56.97 AFV.select :holdingCoil
@04:05:57.07 ATV.dial :extSrc
@04:05:57.09 AS.reconfigure ASslow1
@04:05:57.16 AS.seek 0ml
@04:07:37.99 AFV.select :carrierSolution
@04:07:38.10 delay 2 seconds
@04:07:40.13 AS.reconfigure ASconfig
@04:07:40.18 AS.seek 0.5ml
@04:08:09.23 delay 1 second
@04:08:10.25 AFV.select :holdingCoil
@04:08:10.35 ATV.dial :extSrc
@04:08:10.37 AS.reconfigure ASslow1
@04:08:10.44 AS.seek 0ml
@04:09:51.29 AFV.select :carrierSolution
@04:09:51.41 delay 2 seconds
@04:09:53.44 AS.reconfigure ASconfig
@04:09:53.49 AS.seek 0.5ml
@04:10:22.53 delay 1 second
@04:10:23.55 AFV.select :holdingCoil
@04:10:23.65 ATV.dial :extSrc
@04:10:23.67 AS.reconfigure ASslow1
@04:10:23.74 AS.seek 0ml
@04:12:04.57 ATV.dial :air2
@04:12:05.96 AS.reconfigure ASslow0
@04:12:06.02 AS.seek 0.5ml
@04:12:56.63 ATV.dial :extSrc
@04:12:58.18 AS.seek 0.02ml
@04:13:46.81 delay 1 second
@04:13:47.83 ATV.dial :waste
@04:13:49.20 AS.reconfigure ASconfig
@04:13:49.26 AS.seek 0ml
@04:13:50.71 AFV.select :holdingCoil
@04:13:50.72 ATV.dial :waste
@04:13:50.75 AS.seek 0ml
@04:13:50.83 AFV.select :carrierSolution
@04:13:50.94 delay 2 seconds
@04:13:52.97 AS.seek 0.5ml
@04:14:21.98 delay 1 second
@04:14:23.00 AFV.select :holdingCoil
@04:14:23.10 ATV.dial :air3
@04:14:24.00 AS.reconfigure ASslow1
@04:14:24.06 AS.seek 0.52ml
@04:14:28.46 ATV.dial :air3
@04:14:28.55 AS.seek 0.53ml
@04:14:31.04 delay 2 seconds
@04:14:33.06 ATV.dial :bleach
@04:14:33.55 AS.seek 0.71ml
@04:15:10.01 delay 3 seconds
@04:15:13.03 ATV.dial :air3
@04:15:13.62 delay 3 seconds
@04:15:16.69 AS.seek 0.735ml
@04:15:22.04 delay 3 seconds
@04:15:25.06 ATV.dial :bleach
@04:15:25.71 AS.seek 0.72ml
@04:15:29.17 delay 3 seconds
@04:15:32.19 ATV.dial ASV
@04:15:33.31 ASV.dial :SPErecovery
@04:15:33.44 AS.seek 0.365ml
@04:16:45.13 ATV.dial :waste
@04:16:46.93 AS.reconfigure ASnorm
@04:16:47.00 AS.seek 0ml
@04:17:05.71 ATV.dial :air3
@04:17:06.44 AS.reconfigure ASslow1
@04:17:06.50 AS.seek 0.01ml
@04:17:08.90 delay 2 seconds
@04:17:10.92 ATV.dial :bleach
@04:17:11.41 AS.seek 0.58ml
@04:19:06.32 delay 3 seconds
@04:19:09.34 ATV.dial :air3
@04:19:09.95 delay 3 seconds
@04:19:13.02 AS.seek 0.605ml
@04:19:18.39 delay 3 seconds
@04:19:21.41 ATV.dial :bleach
@04:19:22.05 AS.seek 0.59ml
@04:19:25.34 delay 3 seconds
@04:19:28.36 ATV.dial :ASV
@04:19:29.45 ASV.dial :wasteR
@04:19:29.85 AS.seek 0ml
@04:21:28.79 delay 1:00
@04:22:28.82 ATV.dial ASV
@04:22:28.83 AFV.select :carrierSolution
@04:22:28.95 delay 2 seconds
@04:22:30.97 AS.reconfigure ASconfig
@04:22:31.03 AS.seek 1ml
@04:23:28.79 delay 1 second
@04:23:29.81 AFV.select :holdingCoil
@04:23:29.91 delay 1 second
@04:23:30.93 ASV.dial :wasteR
@04:23:30.95 AS.reconfigure ASslow1
@04:23:31.02 AS.seek 0ml
@04:26:52.42 delay 10 seconds
@04:27:02.45 ATV.dial :air3
@04:27:04.05 AS.seek 0.01ml
@04:27:06.35 delay 2 seconds
@04:27:08.37 ATV.dial :bleach
@04:27:09.04 AS.seek 0.58ml
@04:29:03.99 delay 3 seconds
@04:29:07.01 ATV.dial :air3
@04:29:07.60 delay 3 seconds
@04:29:10.67 AS.seek 0.605ml
@04:29:16.02 delay 3 seconds
@04:29:19.04 ATV.dial :bleach
@04:29:19.69 AS.seek 0.59ml
@04:29:22.98 delay 3 seconds
@04:29:26.00 ATV.dial ASV
@04:29:27.10 ASV.dial :SPEdelivery
@04:29:27.92 AS.seek 0ml
@04:31:26.84 delay 1:00
@04:32:26.87 AFV.select :carrierSolution
@04:32:26.97 delay 2 seconds
@04:32:29.00 AS.reconfigure ASconfig
@04:32:29.05 AS.seek 1ml
@04:33:26.82 delay 1 second
@04:33:27.84 AFV.select :holdingCoil
@04:33:27.94 delay 1 second
@04:33:28.96 ATV.dial ASV
@04:33:28.97 ASV.dial :SPEdelivery
@04:33:28.99 AS.reconfigure ASslow1
@04:33:29.07 AS.seek 0ml
@04:36:50.48 delay 10 seconds
@04:37:00.51 ATV.dial :air2
@04:37:01.38 AS.reconfigure ASslow0
@04:37:01.44 AS.seek 0.8ml
@04:38:22.24 delay 3 seconds
@04:38:25.26 ATV.dial :ASV
@04:38:26.31 ASV.dial :SPEdelivery
@04:38:26.33 AS.reconfigure ASslow1
@04:38:26.40 AS.seek 0.03ml
@04:41:01.56 delay 20 seconds
@04:41:21.59 ATV.dial :waste,via: :air3
@04:41:23.43 AS.reconfigure ASconfig
@04:41:23.49 AS.seek 0ml
@04:41:25.52 SPE.coast
@04:41:25.60 Removing Bleach from Mixing Coil
@04:41:25.61 AFV.select :carrierSolution
@04:41:25.72 delay 2 seconds
@04:41:27.75 AS.seek 0.5ml
@04:41:56.81 delay 1 second
@04:41:57.83 AFV.select :holdingCoil
@04:41:57.93 ATV.dial :ASV
@04:41:59.71 ASV.dial :SPErecovery
@04:42:00.37 AS.reconfigure ASslow1
@04:42:00.43 AS.seek 0.73ml
@04:42:47.02 ASV.dial :wasteD
@04:42:47.62 AS.reconfigure ASnorm
@04:42:47.68 AS.seek 0ml
@04:43:24.81 AFV.select :carrierSolution
@04:43:24.92 delay 2 seconds
@04:43:26.95 AS.reconfigure ASconfig
@04:43:27.00 AS.seek 0.5ml
@04:43:56.02 delay 1 second
@04:43:57.04 AFV.select :holdingCoil
@04:43:57.14 ATV.dial ASV
@04:43:57.16 ASV.dial :SPErecovery
@04:43:57.82 AS.reconfigure ASslow0
@04:43:57.89 AS.seek 0.3ml
@04:44:18.35 ATV.dial :air2
@04:44:19.43 AS.reconfigure ASslow1
@04:44:19.49 AS.seek 0.32ml
@04:44:23.90 ATV.dial ASV
@04:44:24.99 AS.reconfigure ASslow0
@04:44:25.05 AS.seek 0.57ml
@04:44:50.54 ASV.dial :wasteD
@04:44:51.17 AS.reconfigure ASnorm
@04:44:51.24 AS.seek 0ml
@04:45:20.31 AFV.select :carrierSolution
@04:45:20.42 delay 2 seconds
@04:45:22.45 AS.reconfigure ASconfig
@04:45:22.50 AS.seek 0.5ml
@04:45:51.51 delay 1 second
@04:45:52.53 AFV.select :holdingCoil
@04:45:52.63 ATV.dial ASV
@04:45:52.65 ASV.dial :SPErecovery
@04:45:53.32 AS.reconfigure ASslow0
@04:45:53.39 AS.seek 0.3ml
@04:46:13.85 ATV.dial :air2
@04:46:14.92 AS.reconfigure ASslow1
@04:46:14.98 AS.seek 0.32ml
@04:46:19.43 ATV.dial ASV
@04:46:20.53 AS.reconfigure ASslow0
@04:46:20.60 AS.seek 0.57ml
@04:46:46.09 ASV.dial :wasteD
@04:46:46.71 AS.reconfigure ASnorm
@04:46:46.77 AS.seek 0ml
@04:47:15.85 ATV.dial :air2
@04:47:16.87 AS.reconfigure ASslow0
@04:47:16.93 AS.seek 0.3ml
@04:47:47.45 ATV.dial :ASV
@04:47:48.49 ASV.dial :wasteD
@04:47:48.51 AS.reconfigure ASconfig
@04:47:48.57 AS.seek 0.05ml
@04:48:03.26 ATV.dial :waste
@04:48:05.23 AS.seek 0ml
@04:48:08.41 ASV.dial :SPEdelivery
@04:48:09.50 Cleaning PCR
@04:48:09.51 AFV.select :carrierSolution
@04:48:09.63 delay 2 seconds
@04:48:11.66 AS.seek 0.5ml
@04:48:40.68 delay 1 second
@04:48:41.70 AFV.select :holdingCoil
@04:48:41.80 ASV.dial :waste1
@04:48:42.18 ATV.dial :air3
@04:48:42.90 AS.reconfigure ASslow1
@04:48:42.96 AS.seek 0.51ml
@04:48:45.35 delay 2 seconds
@04:48:47.37 ATV.dial :bleach
@04:48:47.85 AS.seek 0.81ml
@04:49:48.46 delay 3 seconds
@04:49:51.48 ATV.dial :air3
@04:49:52.09 delay 3 seconds
@04:49:55.16 AS.seek 0.835ml
@04:50:00.56 delay 3 seconds
@04:50:03.58 ATV.dial :bleach
@04:50:04.66 AS.seek 0.82ml
@04:50:08.07 delay 3 seconds
@04:50:11.09 ARV.dial :waste
@04:50:11.73 delay 3 seconds
@04:50:14.75 ATV.dial :ARV
@04:50:15.68 AS.reconfigure ASconfig
@04:50:15.74 AS.seek 0.47ml
@04:50:36.13 AFV.select :carrierSolution
@04:50:36.24 delay 2 seconds
@04:50:38.27 AS.seek 1ml
@04:51:09.01 delay 1 second
@04:51:10.03 AFV.select :holdingCoil
@04:51:10.13 delay 1 second
@04:51:11.16 AS.reconfigure ASfast
@04:51:11.21 AS.seek 0ml
@04:51:38.57 AFV.select :carrierSolution
@04:51:38.68 delay 2 seconds
@04:51:40.71 AS.reconfigure ASconfig
@04:51:40.76 AS.seek 1ml
@04:52:38.51 delay 1 second
@04:52:39.53 AFV.select :holdingCoil
@04:52:39.63 delay 1 second
@04:52:40.66 AS.reconfigure ASfast
@04:52:40.71 AS.seek 0ml
@04:53:08.07 AFV.select :carrierSolution
@04:53:08.18 delay 2 seconds
@04:53:10.21 AS.reconfigure ASconfig
@04:53:10.26 AS.seek 1ml
@04:54:08.01 delay 1 second
@04:54:09.03 AFV.select :holdingCoil
@04:54:09.13 delay 1 second
@04:54:10.16 AS.reconfigure ASfast
@04:54:10.21 AS.seek 0ml
@04:54:37.59 ARV.dial between carrier and air1
@04:54:38.12 ATV.dial :air3
@04:54:39.48 AS.reconfigure ASslow1
@04:54:39.54 AS.seek 0.01ml
@04:54:41.95 delay 2 seconds
@04:54:43.97 ATV.dial :bleach
@04:54:44.63 AS.seek 0.81ml
@04:57:25.79 delay 3 seconds
@04:57:28.81 ATV.dial :air3
@04:57:29.40 delay 3 seconds
@04:57:32.47 AS.seek 0.835ml
@04:57:37.81 delay 3 seconds
@04:57:40.83 ATV.dial :bleach
@04:57:41.48 AS.seek 0.82ml
@04:57:44.92 delay 3 seconds
@04:57:47.94 ATV.dial PCR
@04:57:48.35 AS.reconfigure ASconfig
@04:57:48.41 AS.seek 0ml
@04:58:35.84 PCR.program :bleachProfile
@04:58:35.90 PCR program should complete in < 2:45
@04:58:35.91 PCR.run 10:00
@05:01:37.77 PCR.off
@05:01:38.22 AFV.select :carrierSolution
@05:01:38.33 delay 2 seconds
@05:01:40.36 AS.seek 1ml
@05:02:38.12 delay 1 second
@05:02:39.14 AFV.select :holdingCoil
@05:02:39.24 delay 1 second
@05:02:40.27 AS.reconfigure ASfast
@05:02:40.32 AS.seek 0ml
@05:03:07.66 delay 3 seconds
@05:03:10.68 AFV.select :carrierSolution
@05:03:10.78 delay 2 seconds
@05:03:12.81 AS.reconfigure ASconfig
@05:03:12.87 AS.seek 1ml
@05:04:10.63 delay 1 second
@05:04:11.65 AFV.select :holdingCoil
@05:04:11.75 delay 1 second
@05:04:12.78 AS.reconfigure ASfast
@05:04:12.83 AS.seek 0ml
@05:04:40.19 delay 3 seconds
@05:04:43.21 AFV.select :carrierSolution
@05:04:43.31 delay 2 seconds
@05:04:45.34 AS.reconfigure ASconfig
@05:04:45.40 AS.seek 1ml
@05:05:43.20 delay 1 second
@05:05:44.22 AFV.select :holdingCoil
@05:05:44.32 delay 1 second
@05:05:45.35 AS.reconfigure ASfast
@05:05:45.40 AS.seek 0ml
@05:06:12.74 delay 3 seconds
@05:06:15.76 AFV.select :carrierSolution
@05:06:15.86 delay 2 seconds
@05:06:17.89 AS.reconfigure ASconfig
@05:06:17.95 AS.seek 1ml
@05:07:15.70 delay 1 second
@05:07:16.72 AFV.select :holdingCoil
@05:07:16.82 delay 1 second
@05:07:17.85 AS.reconfigure ASfast
@05:07:17.90 AS.seek 0ml
@05:07:45.26 delay 3 seconds
@05:07:48.28 AFV.select :carrierSolution
@05:07:48.38 delay 2 seconds
@05:07:50.40 AS.reconfigure ASconfig
@05:07:50.47 AS.seek 1ml
@05:08:48.21 delay 1 second
@05:08:49.23 AFV.select :holdingCoil
@05:08:49.33 delay 1 second
@05:08:50.36 AS.reconfigure ASnorm
@05:08:50.41 AS.seek 0ml
@05:09:41.18 AFV.select :carrierSolution
@05:09:41.28 delay 2 seconds
@05:09:43.30 AS.reconfigure ASconfig
@05:09:43.36 AS.seek 1ml
@05:10:41.12 delay 1 second
@05:10:42.14 AFV.select :holdingCoil
@05:10:42.24 delay 1 second
@05:10:43.26 ATV.dial :waste
@05:10:44.56 AS.reconfigure ASnorm
@05:10:44.62 AS.seek 0.5ml
@05:11:10.20 ATV.dial :waste2
@05:11:11.54 AS.seek 0ml
@05:11:37.15 <MAIN> Cleaning PCR
@05:11:37.16 AFV.select :carrierSolution
@05:11:37.28 delay 2 seconds
@05:11:39.31 AS.reconfigure ASconfig
@05:11:39.36 AS.seek 0.5ml
@05:12:08.40 delay 1 second
@05:12:09.42 AFV.select :holdingCoil
@05:12:09.52 ASV.dial :waste1
@05:12:09.53 ATV.dial :air3
@05:12:11.57 AS.reconfigure ASslow1
@05:12:11.63 AS.seek 0.51ml
@05:12:14.04 delay 2 seconds
@05:12:16.06 ATV.dial :bleach
@05:12:16.54 AS.seek 0.81ml
@05:13:17.15 delay 3 seconds
@05:13:20.17 ATV.dial :air3
@05:13:20.77 delay 3 seconds
@05:13:23.84 AS.seek 0.835ml
@05:13:29.28 delay 3 seconds
@05:13:32.30 ATV.dial :bleach
@05:13:32.94 AS.seek 0.82ml
@05:13:36.37 delay 3 seconds
@05:13:39.39 ARV.dial :waste
@05:13:39.92 delay 3 seconds
@05:13:42.94 ATV.dial :ARV
@05:13:43.86 AS.reconfigure ASconfig
@05:13:43.92 AS.seek 0.47ml
@05:14:04.35 AFV.select :carrierSolution
@05:14:04.46 delay 2 seconds
@05:14:06.49 AS.seek 1ml
@05:14:37.27 delay 1 second
@05:14:38.29 AFV.select :holdingCoil
@05:14:38.39 delay 1 second
@05:14:39.42 AS.reconfigure ASfast
@05:14:39.47 AS.seek 0ml
@05:15:06.81 AFV.select :carrierSolution
@05:15:06.92 delay 2 seconds
@05:15:08.94 AS.reconfigure ASconfig
@05:15:09.00 AS.seek 1ml
@05:16:06.79 delay 1 second
@05:16:07.81 AFV.select :holdingCoil
@05:16:07.91 delay 1 second
@05:16:08.93 AS.reconfigure ASfast
@05:16:08.99 AS.seek 0ml
@05:16:36.32 AFV.select :carrierSolution
@05:16:36.43 delay 2 seconds
@05:16:38.46 AS.reconfigure ASconfig
@05:16:38.51 AS.seek 1ml
@05:17:36.27 delay 1 second
@05:17:37.29 AFV.select :holdingCoil
@05:17:37.39 delay 1 second
@05:17:38.41 AS.reconfigure ASfast
@05:17:38.47 AS.seek 0ml
@05:18:05.81 ARV.dial between carrier and air1
@05:18:06.34 ATV.dial :air3
@05:18:07.68 AS.reconfigure ASslow1
@05:18:07.74 AS.seek 0.01ml
@05:18:10.12 delay 2 seconds
@05:18:12.14 ATV.dial :bleach
@05:18:12.78 AS.seek 0.81ml
@05:20:54.07 delay 3 seconds
@05:20:57.09 ATV.dial :air3
@05:20:57.68 delay 3 seconds
@05:21:00.75 AS.seek 0.835ml
@05:21:06.07 delay 3 seconds
@05:21:09.09 ATV.dial :bleach
@05:21:09.74 AS.seek 0.82ml
@05:21:13.04 delay 3 seconds
@05:21:16.06 ATV.dial PCR
@05:21:16.48 AS.reconfigure ASconfig
@05:21:16.54 AS.seek 0ml
@05:22:03.95 PCR.program :bleachProfile
@05:22:04.40 PCR program should complete in < 2:45
@05:22:04.41 PCR.run 10:00
@05:25:06.32 PCR.off
@05:25:06.36 AFV.select :carrierSolution
@05:25:06.47 delay 2 seconds
@05:25:08.50 AS.seek 1ml
@05:26:06.24 delay 1 second
@05:26:07.26 AFV.select :holdingCoil
@05:26:07.36 delay 1 second
@05:26:08.39 AS.reconfigure ASfast
@05:26:08.44 AS.seek 0ml
@05:26:35.77 delay 3 seconds
@05:26:38.79 AFV.select :carrierSolution
@05:26:38.89 delay 2 seconds
@05:26:40.91 AS.reconfigure ASconfig
@05:26:40.98 AS.seek 1ml
@05:27:38.77 delay 1 second
@05:27:39.79 AFV.select :holdingCoil
@05:27:39.89 delay 1 second
@05:27:40.92 AS.reconfigure ASfast
@05:27:40.97 AS.seek 0ml
@05:28:08.29 delay 3 seconds
@05:28:11.31 AFV.select :carrierSolution
@05:28:11.42 delay 2 seconds
@05:28:13.44 AS.reconfigure ASconfig
@05:28:13.51 AS.seek 1ml
@05:29:11.29 delay 1 second
@05:29:12.31 AFV.select :holdingCoil
@05:29:12.41 delay 1 second
@05:29:13.44 AS.reconfigure ASfast
@05:29:13.49 AS.seek 0ml
@05:29:40.84 delay 3 seconds
@05:29:43.86 AFV.select :carrierSolution
@05:29:43.96 delay 2 seconds
@05:29:45.98 AS.reconfigure ASconfig
@05:29:46.05 AS.seek 1ml
@05:30:43.81 delay 1 second
@05:30:44.83 AFV.select :holdingCoil
@05:30:44.93 delay 1 second
@05:30:45.96 AS.reconfigure ASfast
@05:30:46.01 AS.seek 0ml
@05:31:13.34 delay 3 seconds
@05:31:16.36 AFV.select :carrierSolution
@05:31:16.46 delay 2 seconds
@05:31:18.48 AS.reconfigure ASconfig
@05:31:18.54 AS.seek 1ml
@05:32:16.29 delay 1 second
@05:32:17.31 AFV.select :holdingCoil
@05:32:17.41 delay 1 second
@05:32:18.43 AS.reconfigure ASnorm
@05:32:18.49 AS.seek 0ml
@05:33:09.22 delay 5 seconds
@05:33:14.29 Cleaning PCR
@05:33:14.30 AFV.select :carrierSolution
@05:33:14.42 delay 2 seconds
@05:33:16.45 AS.reconfigure ASconfig
@05:33:16.50 AS.seek 0.5ml
@05:33:45.54 delay 1 second
@05:33:46.56 AFV.select :holdingCoil
@05:33:46.66 ASV.dial :waste1
@05:33:46.67 ATV.dial :air3
@05:33:47.56 AS.reconfigure ASslow1
@05:33:47.62 AS.seek 0.51ml
@05:33:49.90 delay 2 seconds
@05:33:51.92 ATV.dial :bleach
@05:33:52.56 AS.seek 0.81ml
@05:34:53.26 delay 3 seconds
@05:34:56.28 ATV.dial :air3
@05:34:56.87 delay 3 seconds
@05:34:59.94 AS.seek 0.835ml
@05:35:05.24 delay 3 seconds
@05:35:08.26 ATV.dial :bleach
@05:35:09.26 AS.seek 0.82ml
@05:35:12.57 delay 3 seconds
@05:35:15.59 ARV.dial :waste
@05:35:16.12 delay 3 seconds
@05:35:19.14 ATV.dial :ARV
@05:35:20.06 AS.reconfigure ASconfig
@05:35:20.12 AS.seek 0.47ml
@05:35:40.56 AFV.select :carrierSolution
@05:35:40.67 delay 2 seconds
@05:35:42.70 AS.seek 1ml
@05:36:13.45 delay 1 second
@05:36:14.47 AFV.select :holdingCoil
@05:36:14.57 delay 1 second
@05:36:15.60 AS.reconfigure ASfast
@05:36:15.65 AS.seek 0ml
@05:36:42.99 AFV.select :carrierSolution
@05:36:43.10 delay 2 seconds
@05:36:45.13 AS.reconfigure ASconfig
@05:36:45.18 AS.seek 1ml
@05:37:43.01 delay 1 second
@05:37:44.03 AFV.select :holdingCoil
@05:37:44.13 delay 1 second
@05:37:45.16 AS.reconfigure ASfast
@05:37:45.21 AS.seek 0ml
@05:38:12.56 AFV.select :carrierSolution
@05:38:12.67 delay 2 seconds
@05:38:14.69 AS.reconfigure ASconfig
@05:38:14.75 AS.seek 1ml
@05:39:12.54 delay 1 second
@05:39:13.56 AFV.select :holdingCoil
@05:39:13.66 delay 1 second
@05:39:14.69 AS.reconfigure ASfast
@05:39:14.74 AS.seek 0ml
@05:39:42.09 ARV.dial between carrier and air1
@05:39:42.62 ATV.dial :air3
@05:39:43.97 AS.reconfigure ASslow1
@05:39:44.02 AS.seek 0.01ml
@05:39:46.48 delay 2 seconds
@05:39:48.50 ATV.dial :bleach
@05:39:49.16 AS.seek 0.81ml
@05:42:30.54 delay 3 seconds
@05:42:33.56 ATV.dial :air3
@05:42:34.16 delay 3 seconds
@05:42:37.23 AS.seek 0.835ml
@05:42:42.56 delay 3 seconds
@05:42:45.58 ATV.dial :bleach
@05:42:46.22 AS.seek 0.82ml
@05:42:49.48 delay 3 seconds
@05:42:52.50 ATV.dial PCR
@05:42:52.93 AS.reconfigure ASconfig
@05:42:52.99 AS.seek 0ml
@05:43:40.40 PCR.program :bleachProfile
@05:43:40.46 PCR program should complete in < 2:45
@05:43:40.47 PCR.run 10:00
@05:46:39.33 PCR.off
@05:46:39.38 AFV.select :carrierSolution
@05:46:39.49 delay 2 seconds
@05:46:41.52 AS.seek 1ml
@05:47:39.26 delay 1 second
@05:47:40.28 AFV.select :holdingCoil
@05:47:40.38 delay 1 second
@05:47:41.40 AS.reconfigure ASfast
@05:47:41.46 AS.seek 0ml
@05:48:08.81 delay 3 seconds
@05:48:12.17 AFV.select :carrierSolution
@05:48:12.29 delay 2 seconds
@05:48:14.31 AS.reconfigure ASconfig
@05:48:14.38 AS.seek 1ml
@05:49:12.12 delay 1 second
@05:49:13.14 AFV.select :holdingCoil
@05:49:13.24 delay 1 second
@05:49:14.27 AS.reconfigure ASfast
@05:49:14.32 AS.seek 0ml
@05:49:41.65 delay 3 seconds
@05:49:44.67 AFV.select :carrierSolution
@05:49:44.77 delay 2 seconds
@05:49:46.79 AS.reconfigure ASconfig
@05:49:46.86 AS.seek 1ml
@05:50:44.62 delay 1 second
@05:50:45.64 AFV.select :holdingCoil
@05:50:45.74 delay 1 second
@05:50:46.77 AS.reconfigure ASfast
@05:50:46.82 AS.seek 0ml
@05:51:14.16 delay 3 seconds
@05:51:17.18 AFV.select :carrierSolution
@05:51:17.28 delay 2 seconds
@05:51:19.30 AS.reconfigure ASconfig
@05:51:19.37 AS.seek 1ml
@05:52:17.12 delay 1 second
@05:52:18.14 AFV.select :holdingCoil
@05:52:18.24 delay 1 second
@05:52:19.27 AS.reconfigure ASfast
@05:52:19.32 AS.seek 0ml
@05:52:46.66 delay 3 seconds
@05:52:49.68 AFV.select :carrierSolution
@05:52:49.78 delay 2 seconds
@05:52:51.80 AS.reconfigure ASconfig
@05:52:51.87 AS.seek 1ml
@05:53:49.60 delay 1 second
@05:53:50.62 AFV.select :holdingCoil
@05:53:50.72 delay 1 second
@05:53:51.74 AS.reconfigure ASnorm
@05:53:51.80 AS.seek 0ml
@05:54:42.54 delay 5 seconds
@05:54:47.65 Cleaning PCR
@05:54:47.66 AFV.select :carrierSolution
@05:54:47.78 delay 2 seconds
@05:54:49.81 AS.reconfigure ASconfig
@05:54:49.86 AS.seek 0.5ml
@05:55:18.90 delay 1 second
@05:55:19.92 AFV.select :holdingCoil
@05:55:20.02 ASV.dial :waste1
@05:55:20.03 ATV.dial :air3
@05:55:20.93 AS.reconfigure ASslow1
@05:55:20.99 AS.seek 0.51ml
@05:55:23.34 delay 2 seconds
@05:55:25.36 ATV.dial :bleach
@05:55:26.01 AS.seek 0.81ml
@05:56:26.60 delay 3 seconds
@05:56:29.62 ATV.dial :air3
@05:56:30.21 delay 3 seconds
@05:56:33.28 AS.seek 0.835ml
@05:56:38.71 delay 3 seconds
@05:56:41.73 ATV.dial :bleach
@05:56:42.37 AS.seek 0.82ml
@05:56:45.65 delay 3 seconds
@05:56:48.67 ARV.dial :waste
@05:56:49.20 delay 3 seconds
@05:56:52.22 ATV.dial :ARV
@05:56:53.14 AS.reconfigure ASconfig
@05:56:53.20 AS.seek 0.47ml
@05:57:13.62 AFV.select :carrierSolution
@05:57:13.73 delay 2 seconds
@05:57:15.76 AS.seek 1ml
@05:57:46.51 delay 1 second
@05:57:47.53 AFV.select :holdingCoil
@05:57:47.63 delay 1 second
@05:57:48.65 AS.reconfigure ASfast
@05:57:48.71 AS.seek 0ml
@05:58:16.06 AFV.select :carrierSolution
@05:58:16.17 delay 2 seconds
@05:58:18.19 AS.reconfigure ASconfig
@05:58:18.25 AS.seek 1ml
@05:59:16.02 delay 1 second
@05:59:17.04 AFV.select :holdingCoil
@05:59:17.14 delay 1 second
@05:59:18.17 AS.reconfigure ASfast
@05:59:18.22 AS.seek 0ml
@05:59:45.56 AFV.select :carrierSolution
@05:59:45.67 delay 2 seconds
@05:59:47.69 AS.reconfigure ASconfig
@05:59:47.75 AS.seek 1ml
@06:00:45.51 delay 1 second
@06:00:46.53 AFV.select :holdingCoil
@06:00:46.63 delay 1 second
@06:00:47.65 AS.reconfigure ASfast
@06:00:47.71 AS.seek 0ml
@06:01:15.04 ARV.dial between carrier and air1
@06:01:15.57 ATV.dial :air3
@06:01:16.94 AS.reconfigure ASslow1
@06:01:17.00 AS.seek 0.01ml
@06:01:19.49 delay 2 seconds
@06:01:21.51 ATV.dial :bleach
@06:01:22.16 AS.seek 0.81ml
@06:04:03.41 delay 3 seconds
@06:04:06.43 ATV.dial :air3
@06:04:07.02 delay 3 seconds
@06:04:10.09 AS.seek 0.835ml
@06:04:15.48 delay 3 seconds
@06:04:18.50 ATV.dial :bleach
@06:04:19.14 AS.seek 0.82ml
@06:04:22.54 delay 3 seconds
@06:04:25.56 ATV.dial PCR
@06:04:25.98 AS.reconfigure ASconfig
@06:04:26.04 AS.seek 0ml
@06:05:13.46 PCR.program :bleachProfile
@06:05:13.52 PCR program should complete in < 2:45
@06:05:13.53 PCR.run 10:00
@06:08:12.72 PCR.off
@06:08:12.76 AFV.select :carrierSolution
@06:08:12.87 delay 2 seconds
@06:08:14.90 AS.seek 1ml
@06:09:12.70 delay 1 second
@06:09:13.72 AFV.select :holdingCoil
@06:09:13.82 delay 1 second
@06:09:14.85 AS.reconfigure ASfast
@06:09:14.90 AS.seek 0ml
@06:09:42.23 delay 3 seconds
@06:09:45.25 AFV.select :carrierSolution
@06:09:45.35 delay 2 seconds
@06:09:47.37 AS.reconfigure ASconfig
@06:09:47.44 AS.seek 1ml
@06:10:45.18 delay 1 second
@06:10:46.20 AFV.select :holdingCoil
@06:10:46.30 delay 1 second
@06:10:47.33 AS.reconfigure ASfast
@06:10:47.38 AS.seek 0ml
@06:11:14.73 delay 3 seconds
@06:11:17.75 AFV.select :carrierSolution
@06:11:17.85 delay 2 seconds
@06:11:19.87 AS.reconfigure ASconfig
@06:11:19.94 AS.seek 1ml
@06:12:17.66 delay 1 second
@06:12:18.68 AFV.select :holdingCoil
@06:12:18.78 delay 1 second
@06:12:19.81 AS.reconfigure ASfast
@06:12:19.86 AS.seek 0ml
@06:12:47.20 delay 3 seconds
@06:12:50.22 AFV.select :carrierSolution
@06:12:50.32 delay 2 seconds
@06:12:52.34 AS.reconfigure ASconfig
@06:12:52.41 AS.seek 1ml
@06:13:50.21 delay 1 second
@06:13:51.23 AFV.select :holdingCoil
@06:13:51.33 delay 1 second
@06:13:52.36 AS.reconfigure ASfast
@06:13:52.41 AS.seek 0ml
@06:14:19.73 delay 3 seconds
@06:14:22.75 AFV.select :carrierSolution
@06:14:22.85 delay 2 seconds
@06:14:24.87 AS.reconfigure ASconfig
@06:14:24.94 AS.seek 1ml
@06:15:22.71 delay 1 second
@06:15:23.73 AFV.select :holdingCoil
@06:15:23.83 delay 1 second
@06:15:24.85 AS.reconfigure ASnorm
@06:15:24.91 AS.seek 0ml
@06:16:15.65 delay 5 seconds
@06:16:20.73 <bkha> Session Ended
@10:07:46.92CEST26-Apr-21 <MAIN> ESP2 v3.30 I2C Gateway v3.06 for core bus
@10:07:49.06 
Configuration for mer 7/20/20 brent@mbari.org
/home/esp/esp2/type/shallow/mer/configure.rb
@10:07:49.15 
Configuration for MFB3 by preston@mbari.org
/home/esp/esp2/type/mfb/MFB3/configure.rb
@10:07:53.50 Polling Can every 10:00
@10:07:55.21 CS.configure :default
@10:07:55.25 CS.hold
@10:07:55.32 CS.configure :default
@10:07:55.40 PS.configure :default
@10:07:55.48 SS.configure :default
@10:07:55.51 SS.hold
@10:07:55.57 SS.configure :default
@10:07:55.65 CC.configure :default
@10:07:55.68 CC.hold
@10:07:55.75 CC.configure :default
@10:07:55.83 PC.configure :default
@10:07:55.86 PC.hold
@10:07:55.93 PC.configure :default
@10:07:56.02 Forearm.configure :loose
@10:07:56.06 Forearm.hold
@10:07:56.12 Forearm.configure :loose
@10:07:56.19 Elbow.configure :loose
@10:07:56.23 Elbow.hold
@10:07:56.27 Elbow.configure :loose
@10:07:56.37 SC.configure :loose
@10:07:56.41 SC.hold
@10:07:56.47 SC.configure :loose
@10:07:56.54 SE.configure :default
@10:07:56.58 SE.hold
@10:07:56.64 SE.configure :default
@10:07:56.67 CH.coast
@10:07:56.72 CH.configure :default
@10:07:56.74 PH.coast
@10:07:56.79 PH.configure :default
@10:07:56.89 SPE.coast
@10:07:56.94 SPE.configure SPEconfig
@10:07:57.01 AS.configure ASconfig
@10:07:57.05 AS.hold
@10:07:57.11 AS.configure ASconfig
@10:07:57.15 PCR.off
@10:07:57.18 PCR.pidtemp= 50,0.1,0,300,25,25
@10:07:57.21 PCR.caltemp= 2.5051,0.0372,0
@10:07:57.24 All dwarf controllers running firmware version 3.75
@10:07:57.46 Cleanly exited at 10:07:08CEST26-Apr-21
@10:07:57.49 Loading /home/esp/esp2/mission/GENOMAP.rb mission
@10:07:57.64 <MFBPCR> Extracting Nucleic Acid from extSrc
@10:07:57.66 SPE.hold 2:46:40,55C
@10:07:57.70 ASV.dial :SPEdelivery
@10:07:58.16 delay 20 seconds
@10:07:58.54 <bspe> Session Begun
@10:08:18.20 <MFBPCR> AFV.select :holdingCoil
@10:08:18.32 ATV.dial :air2
@10:08:19.60 AS.reconfigure ASslow1
@10:08:19.67 AS.seek 0.07ml
@10:08:34.08 delay 3 seconds
@10:08:37.10 ATV.dial :extSrc
@10:08:38.61 AS.seek 0.47ml
@10:09:45.05 <bspe> -> exit
@10:09:45.07 Session Ended
@10:09:59.33 <MFBPCR> delay 5 seconds
@10:10:04.40 Solid Phase Extraction Heater at 39.7C
@10:10:04.41 ATV.dial ASV
@10:10:05.28 ASV.dial :SPEdelivery
@10:10:05.35 AS.seek 0.042ml
@10:11:31.88 delay 10 seconds
@10:11:41.92 Rinsing external lysate line extSrc
@10:11:41.93 AFV.select :carrierSolution
@10:11:42.03 delay 2 seconds
@10:11:44.05 AS.reconfigure ASconfig
@10:11:44.10 AS.seek 1ml
@10:11:52.23 <mwj> Session Begun
@10:12:00.07 -> showlog 10
@10:12:39.44 <MFBPCR> delay 1 second
@10:12:40.46 AFV.select :holdingCoil
@10:12:40.56 delay 1 second
@10:12:41.58 ATV.dial :extSrc
@10:12:42.42 AS.reconfigure ASnorm
@10:12:42.49 AS.seek 0ml
@10:13:33.17 ATV.dial :air2
@10:13:34.78 AS.reconfigure ASslow0
@10:13:34.84 AS.seek 0.05ml
@10:13:40.23 ATV.dial :extSrc
@10:13:41.83 AS.seek 0.01ml
@10:13:46.60 ATV.dial :waste
@10:13:47.97 AS.reconfigure ASconfig
@10:13:48.03 AS.seek 0ml
@10:13:48.91 ethanol wash
@10:13:48.92 ATV.dial :air2
@10:13:50.11 AS.reconfigure ASslow2
@10:13:50.18 AS.seek 0.01ml
@10:14:00.91 delay 3 seconds
@10:14:03.93 Pulling 0.4ml of Ethanol Wash
@10:14:03.94 ATV.dial :ethanol
@10:14:04.83 AS.reconfigure ASslow1
@10:14:04.89 AS.seek 0.41ml
@10:14:18.66 <mwj> -> exit
@10:14:18.68 Session Ended
@10:15:25.64 <MFBPCR> delay 3 seconds
@10:15:28.66 ATV.dial :air1
@10:15:29.08 delay 3 seconds
@10:15:32.15 AS.seek 0.435ml
@10:15:37.43 delay 3 seconds
@10:15:40.45 ATV.dial :ethanol
@10:15:41.11 AS.seek 0.42ml
@10:15:44.52 delay 3 seconds
@10:15:47.54 ATV.dial ASV
@10:15:48.93 AS.seek 0.093ml
@10:16:55.09 delay 3 seconds
@10:16:58.11 ATV.dial :waste2
@10:16:58.91 AS.reconfigure ASconfig
@10:16:58.95 AS.seek 0ml
@10:17:04.61 ATV.dial :air2
@10:17:05.02 AS.reconfigure ASslow1
@10:17:05.07 AS.seek 1ml
@10:20:26.44 delay 5 seconds
@10:20:31.46 ATV.dial ASV
@10:20:32.48 ASV.dial :SPEdelivery
@10:20:32.50 AS.seek 0.02ml
@10:23:49.98 delay 5 seconds
@10:23:55.00 ATV.dial :air2
@10:23:56.08 AS.seek 0.52ml
@10:25:36.91 delay 5 seconds
@10:25:41.93 ATV.dial ASV
@10:25:42.97 ASV.dial :wasteR
@10:25:43.34 AS.seek 0.3ml
@10:26:27.88 delay 20 seconds
@10:26:47.93 AS.seek 0.07ml
@10:27:34.58 delay 20 seconds
@10:27:54.60 ATV.dial :waste2
@10:27:55.39 AS.reconfigure ASconfig
@10:27:55.43 AS.seek 0ml
@10:27:59.75 Elution
@10:27:59.76 ATV.dial :air1
@10:28:00.93 AS.reconfigure ASslow1
@10:28:00.98 AS.seek 0.05ml
@10:28:11.30 delay 3 seconds
@10:28:14.32 ATV.dial :ElutionWater
@10:28:15.02 AS.seek 0.11ml
@10:28:27.41 delay 3 seconds
@10:28:30.43 ATV.dial ASV,via: :air2
@10:28:32.67 ASV.dial :SPEelution
@10:28:33.28 AFV.select :carrierSolution
@10:28:33.43 AS.reconfigure ASnorm
@10:28:33.47 AS.seek 0.76ml
@10:29:06.55 delay 3 seconds
@10:29:09.57 AFV.select :holdingCoil
@10:29:09.72 AS.reconfigure ASslow1
@10:29:09.76 AS.seek 0.475ml
@10:30:07.33 delay 5 seconds
@10:30:12.41 AS.reconfigure ASslow2
@10:30:12.45 AS.seek 0.44ml
@10:30:48.00 delay 1:00
@10:31:48.03 pushing eluate to holding coil
@10:31:48.09 AS.seek 0.28ml
@10:34:29.64 delay 30 seconds
@10:34:59.67 ATV.dial :waste,via: :air3
@10:35:01.50 AS.reconfigure ASconfig
@10:35:01.54 AS.seek 0ml
@10:35:17.94 Parking Bleach in LysateSource line and SPEcolumn
@10:35:17.95 ATV.dial :air3
@10:35:18.82 AS.reconfigure ASslow1
@10:35:18.86 AS.seek 0.03ml
@10:35:25.31 ATV.dial :ElutionWater
@10:35:25.93 AS.seek 0.06ml
@10:35:32.23 ATV.dial :air3
@10:35:32.86 AS.seek 0.07ml
@10:35:35.16 delay 2 seconds
@10:35:37.18 ATV.dial :bleach
@10:35:37.64 AS.seek 0.15ml
@10:35:54.00 delay 3 seconds
@10:35:57.02 ATV.dial :air3
@10:35:57.61 delay 3 seconds
@10:36:00.68 AS.seek 0.175ml
@10:36:06.07 delay 3 seconds
@10:36:09.09 ATV.dial :bleach
@10:36:09.72 AS.seek 0.16ml
@10:36:13.11 delay 3 seconds
@10:36:16.13 ATV.dial :extSrc
@10:36:16.77 AS.reconfigure ASconfig
@10:36:16.82 AS.seek 0.015ml
@10:36:25.45 ATV.dial :waste
@10:36:26.98 AS.seek 0ml
@10:36:28.16 ATV.dial :air3
@10:36:29.02 AS.reconfigure ASslow1
@10:36:29.07 AS.seek 0.01ml
@10:36:31.47 delay 2 seconds
@10:36:33.49 ATV.dial :bleach
@10:36:33.96 AS.seek 0.58ml
@10:38:28.87 delay 3 seconds
@10:38:31.89 ATV.dial :air3
@10:38:32.48 delay 3 seconds
@10:38:35.55 AS.seek 0.605ml
@10:38:40.93 delay 3 seconds
@10:38:43.95 ATV.dial :bleach
@10:38:44.58 AS.seek 0.59ml
@10:38:47.98 delay 3 seconds
@10:38:51.00 ATV.dial :ASV
@10:38:52.09 ASV.dial :wasteR
@10:38:52.70 AS.seek 0ml
@10:40:51.73 delay 1:00
@10:41:51.76 AFV.select :carrierSolution
@10:41:51.86 delay 2 seconds
@10:41:53.88 AS.reconfigure ASconfig
@10:41:53.92 AS.seek 1ml
@10:42:51.64 delay 1 second
@10:42:52.66 AFV.select :holdingCoil
@10:42:52.76 delay 1 second
@10:42:53.78 ATV.dial ASV
@10:42:53.79 ASV.dial :wasteR
@10:42:53.81 AS.reconfigure ASslow1
@10:42:53.85 AS.seek 0ml
@10:46:15.27 delay 20 seconds
@10:46:35.30 ATV.dial :air3
@10:46:36.88 AS.seek 0.59ml
@10:48:35.97 delay 3 seconds
@10:48:39.00 ATV.dial :ASV
@10:48:40.50 ASV.dial :wasteR
@10:48:40.57 AS.seek 0.09ml
@10:50:21.45 delay 30 seconds
@10:50:51.48 ATV.dial :extSrc
@10:50:52.30 delay 3 seconds
@10:50:55.32 ATV.dial :ElutionWater
@10:50:56.47 AS.seek 0.12ml
@10:51:02.80 ATV.dial :air3
@10:51:03.41 AS.seek 0.13ml
@10:51:05.67 delay 2 seconds
@10:51:07.69 ATV.dial :bleach
@10:51:08.18 AS.seek 0.23ml
@10:51:28.67 delay 3 seconds
@10:51:31.69 ATV.dial :air3
@10:51:32.27 delay 3 seconds
@10:51:35.34 AS.seek 0.255ml
@10:51:40.66 delay 3 seconds
@10:51:43.68 ATV.dial :bleach
@10:51:44.73 AS.seek 0.24ml
@10:51:48.12 delay 3 seconds
@10:51:51.14 ATV.dial :ASV
@10:51:52.23 ASV.dial :SPEdelivery
@10:51:52.62 AFV.select :carrierSolution
@10:51:52.72 delay 2 seconds
@10:51:54.75 AS.reconfigure ASconfig
@10:51:54.79 AS.seek 0.5ml
@10:52:10.02 delay 1 second
@10:52:11.04 AFV.select :holdingCoil
@10:52:11.19 AS.reconfigure ASslow1
@10:52:11.23 AS.seek 0.17ml
@10:53:17.87 SPE.hold 2:46:40,95C
@10:53:17.91 delay 3:00
@10:56:17.96 AFV.select :carrierSolution
@10:56:18.06 delay 2 seconds
@10:56:20.08 AS.reconfigure ASconfig
@10:56:20.13 AS.seek 1ml
@10:57:08.12 delay 1 second
@10:57:09.14 AFV.select :holdingCoil
@10:57:09.24 delay 1 second
@10:57:10.26 ASV.dial :wasteD
@10:57:11.36 AS.seek 0ml
@10:58:09.12 Priming Eluate
@10:58:09.13 AFV.select :carrierSolution
@10:58:09.23 delay 2 seconds
@10:58:11.26 AS.seek 0.5ml
@10:58:40.25 delay 1 second
@10:58:41.27 AFV.select :holdingCoil
@10:58:41.37 ATV.dial ASV
@10:58:41.38 ASV.dial :SPErecovery
@10:58:42.00 Swish #1 of 2
@10:58:42.06 AS.reconfigure ASslow0
@10:58:42.11 AS.seek 0.6ml
@10:58:52.48 AS.seek 0.5ml
@10:59:02.81 Swish #2 of 2
@10:59:02.87 AS.seek 0.6ml
@10:59:13.33 AS.seek 0.5ml
@10:59:23.67 delay 3 seconds
@10:59:26.74 AS.reconfigure ASslow1
@10:59:26.78 AS.seek 0.684ml
@11:00:04.12 delay 3 seconds
@11:00:07.14 Eluate primed
@11:00:07.15 ATV.dial :waste2
@11:00:07.80 AS.reconfigure ASnorm
@11:00:07.84 AS.seek 0ml
@11:00:42.64 SPE.coast
@11:00:42.70 ASV.dial :SPErecovery
@11:00:42.72 Setting up PCR for ACYPP2 -- writing /var/log/esp/ACYPP221apr2611h.pcr
@11:00:42.73 Building PCR Slug from ASV with MM1 mix, PP2 primer
@11:00:42.75 Priming with carrier
@11:00:42.76 ATV.dial ARV
@11:00:43.78 ARV.dial :carrier
@11:00:44.14 AS.seek 0.5ml
@11:01:09.66 AFV.select :carrierSolution
@11:01:09.76 delay 2 seconds
@11:01:11.79 AS.reconfigure ASconfig
@11:01:11.83 AS.seek 1ml
@11:01:40.83 delay 1 second
@11:01:41.85 AFV.select :holdingCoil
@11:01:41.95 delay 1 second
@11:01:42.97 ATV.dial :waste
@11:01:44.56 AS.reconfigure ASnorm
@11:01:44.61 AS.seek 0ml
@11:02:35.28 AFV.select :carrierSolution
@11:02:35.39 delay 2 seconds
@11:02:37.42 AS.reconfigure ASconfig
@11:02:37.46 AS.seek 0.5ml
@11:03:06.53 delay 1 second
@11:03:07.55 AFV.select :holdingCoil
@11:03:07.65 Building Slug
@11:03:07.66 ATV.dial :ARV
@11:03:09.39 delay 2 seconds
@11:03:11.41 ARV.dial :air1
@11:03:11.81 delay 2 seconds
@11:03:13.88 AS.reconfigure ASslow2
@11:03:13.93 AS.seek 0.506ml
@11:03:20.30 delay 2 seconds
@11:03:22.32 ARV.dial :MM1
@11:03:22.95 delay 2 seconds
@11:03:24.97 Adding MM1
@11:03:25.03 AS.seek 0.524ml
@11:03:43.48 delay 25 seconds
@11:04:08.50 ARV.dial :PP2
@11:04:08.92 Adding PP2
@11:04:08.93 delay 2 seconds
@11:04:11.00 AS.seek 0.53ml
@11:04:17.61 delay 10 seconds
@11:04:27.64 ARV.dial :air2
@11:04:28.05 adding ARV air
@11:04:28.06 delay 2 seconds
@11:04:30.13 AS.seek 0.536ml
@11:04:36.30 delay 5 seconds
@11:04:41.32 ARV.dial :carrier
@11:04:42.69 delay 7 seconds
@11:04:49.77 AS.seek 0.594ml
@11:05:48.53 delay 5 seconds
@11:05:53.55 ATV.dial :ASV
@11:05:53.97 delay 2 seconds
@11:05:56.04 AS.seek 0.6ml
@11:06:02.34 delay 4 seconds
@11:06:06.36 ATV.dial ARV
@11:06:06.94 delay 2 seconds
@11:06:08.96 ARV.dial :carrier
@11:06:08.97 delay 2 seconds
@11:06:11.04 AS.seek 0.65ml
@11:07:01.88 delay 4 seconds
@11:07:05.90 ATV.dial PCR
@11:07:06.55 ASV.dial :waste1
@11:07:07.37 ARV.dial :air1
@11:07:07.78 Positioning Slug by 0.324ml
@11:07:07.85 AS.seek 0.326ml
@11:12:34.33 delay 5 seconds
@11:12:39.35 ATV.dial ARV
@11:12:40.16 ASV.dial :PCRlock
@11:12:40.53 delay 5 seconds
@11:12:45.55 Running ACYPP2 Profile60CIQ2 thermocycling
@11:12:45.57 <PCR> PCR.program Profile60CIQ2
@11:12:45.67 Profile60CIQ2 should complete in < 1:47:50
@11:12:45.68 PCR.run 2:19:47.5
@12:41:30.68 PCR.off
@12:41:31.43 <MFBPCR> Running ACYPP2 MeltProfile60 thermocycling
@12:41:31.44 <PCR> PCR.program MeltProfile60
@12:41:31.53 MeltProfile60 should complete in < 11:55
@12:41:31.54 PCR.run 19:53.75
@12:49:15.59 PCR.off
@12:49:16.50 <MFBPCR> PCR for ACYPP2 completed -- closed /var/log/esp/ACYPP221apr2611h.pcr
@12:49:16.56 Cleaning PCR
@12:49:16.57 AFV.select :carrierSolution
@12:49:16.67 delay 2 seconds
@12:49:18.70 AS.reconfigure ASconfig
@12:49:18.74 AS.seek 0.5ml
@12:49:29.03 delay 1 second
@12:49:30.05 AFV.select :holdingCoil
@12:49:30.15 ASV.dial :waste1
@12:49:30.55 ATV.dial :air3
@12:49:31.89 AS.reconfigure ASslow1
@12:49:31.94 AS.seek 0.51ml
@12:49:34.34 delay 2 seconds
@12:49:36.36 ATV.dial :bleach
@12:49:37.01 AS.seek 0.81ml
@12:50:37.62 delay 3 seconds
@12:50:40.64 ATV.dial :air3
@12:50:41.22 delay 3 seconds
@12:50:44.29 AS.seek 0.835ml
@12:50:49.56 delay 3 seconds
@12:50:52.58 ATV.dial :bleach
@12:50:53.21 AS.seek 0.82ml
@12:50:56.48 delay 3 seconds
@12:50:59.50 ARV.dial :waste
@12:51:00.14 delay 3 seconds
@12:51:03.16 ATV.dial :ARV
@12:51:04.06 AS.reconfigure ASconfig
@12:51:04.11 AS.seek 0.47ml
@12:51:24.51 AFV.select :carrierSolution
@12:51:24.61 delay 2 seconds
@12:51:26.64 AS.seek 1ml
@12:51:57.37 delay 1 second
@12:51:58.39 AFV.select :holdingCoil
@12:51:58.49 delay 1 second
@12:51:59.52 AS.reconfigure ASfast
@12:51:59.56 AS.seek 0ml
@12:52:26.89 AFV.select :carrierSolution
@12:52:26.99 delay 2 seconds
@12:52:29.01 AS.reconfigure ASconfig
@12:52:29.05 AS.seek 1ml
@12:53:26.83 delay 1 second
@12:53:27.85 AFV.select :holdingCoil
@12:53:27.95 delay 1 second
@12:53:28.98 AS.reconfigure ASfast
@12:53:29.02 AS.seek 0ml
@12:53:56.36 AFV.select :carrierSolution
@12:53:56.46 delay 2 seconds
@12:53:58.48 AS.reconfigure ASconfig
@12:53:58.53 AS.seek 1ml
@12:54:56.31 delay 1 second
@12:54:57.33 AFV.select :holdingCoil
@12:54:57.43 delay 1 second
@12:54:58.46 AS.reconfigure ASfast
@12:54:58.50 AS.seek 0ml
@12:55:25.83 ARV.dial between carrier and air1
@12:55:26.34 ATV.dial :air3
@12:55:27.67 AS.reconfigure ASslow1
@12:55:27.72 AS.seek 0.01ml
@12:55:30.12 delay 2 seconds
@12:55:32.14 ATV.dial :bleach
@12:55:32.77 AS.seek 0.81ml
@12:58:13.95 delay 3 seconds
@12:58:16.97 ATV.dial :air3
@12:58:17.55 delay 3 seconds
@12:58:20.62 AS.seek 0.835ml
@12:58:25.90 delay 3 seconds
@12:58:28.92 ATV.dial :bleach
@12:58:29.55 AS.seek 0.82ml
@12:58:32.87 delay 3 seconds
@12:58:35.89 ATV.dial PCR
@12:58:36.30 AS.reconfigure ASconfig
@12:58:36.34 AS.seek 0ml
@12:59:23.75 PCR.program :bleachProfile
@12:59:23.81 PCR program should complete in < 2:45
@12:59:23.82 PCR.run 10:00
@13:02:20.00 PCR.off
@13:02:20.03 AFV.select :carrierSolution
@13:02:20.13 delay 2 seconds
@13:02:22.16 AS.seek 1ml
@13:03:19.90 delay 1 second
@13:03:20.92 AFV.select :holdingCoil
@13:03:21.02 delay 1 second
@13:03:22.05 AS.reconfigure ASfast
@13:03:22.09 AS.seek 0ml
@13:03:49.42 delay 3 seconds
@13:03:52.44 AFV.select :carrierSolution
@13:03:52.54 delay 2 seconds
@13:03:54.56 AS.reconfigure ASconfig
@13:03:54.61 AS.seek 1ml
@13:04:52.34 delay 1 second
@13:04:53.36 AFV.select :holdingCoil
@13:04:53.46 delay 1 second
@13:04:54.49 AS.reconfigure ASfast
@13:04:54.53 AS.seek 0ml
@13:05:21.86 delay 3 seconds
@13:05:24.88 AFV.select :carrierSolution
@13:05:24.98 delay 2 seconds
@13:05:27.00 AS.reconfigure ASconfig
@13:05:27.05 AS.seek 1ml
@13:06:24.79 delay 1 second
@13:06:25.81 AFV.select :holdingCoil
@13:06:25.91 delay 1 second
@13:06:26.94 AS.reconfigure ASfast
@13:06:26.98 AS.seek 0ml
@13:06:54.29 delay 3 seconds
@13:06:57.31 AFV.select :carrierSolution
@13:06:57.41 delay 2 seconds
@13:06:59.43 AS.reconfigure ASconfig
@13:06:59.49 AS.seek 1ml
@13:07:57.22 delay 1 second
@13:07:58.24 AFV.select :holdingCoil
@13:07:58.34 delay 1 second
@13:07:59.37 AS.reconfigure ASfast
@13:07:59.42 AS.seek 0ml
@13:08:26.75 delay 3 seconds
@13:08:29.77 AFV.select :carrierSolution
@13:08:29.87 delay 2 seconds
@13:08:31.89 AS.reconfigure ASconfig
@13:08:31.94 AS.seek 1ml
@13:09:29.67 delay 1 second
@13:09:30.69 AFV.select :holdingCoil
@13:09:30.79 delay 1 second
@13:09:31.82 AS.reconfigure ASnorm
@13:09:31.86 AS.seek 0ml
@13:10:22.55 Setting up PCR for AOPP1 -- writing /var/log/esp/AOPP121apr2613h.pcr
@13:10:22.57 Building PCR Slug from ASV with MM1 mix, PP1 primer
@13:10:22.58 Priming with carrier
@13:10:22.59 ATV.dial ARV
@13:10:23.22 ARV.dial :carrier
@13:10:23.56 AS.seek 0.5ml
@13:10:49.22 AFV.select :carrierSolution
@13:10:49.32 delay 2 seconds
@13:10:51.35 AS.reconfigure ASconfig
@13:10:51.39 AS.seek 1ml
@13:11:20.39 delay 1 second
@13:11:21.41 AFV.select :holdingCoil
@13:11:21.51 delay 1 second
@13:11:22.53 ATV.dial :waste
@13:11:24.29 AS.reconfigure ASnorm
@13:11:24.34 AS.seek 0ml
@13:12:15.03 AFV.select :carrierSolution
@13:12:15.13 delay 2 seconds
@13:12:17.16 AS.reconfigure ASconfig
@13:12:17.20 AS.seek 0.5ml
@13:12:46.25 delay 1 second
@13:12:47.27 AFV.select :holdingCoil
@13:12:47.37 Building Slug
@13:12:47.39 ATV.dial :ARV
@13:12:49.11 delay 2 seconds
@13:12:51.13 ARV.dial :air1
@13:12:51.51 delay 2 seconds
@13:12:53.58 AS.reconfigure ASslow2
@13:12:53.62 AS.seek 0.506ml
@13:13:00.00 delay 2 seconds
@13:13:02.02 ARV.dial :MM1
@13:13:02.65 delay 2 seconds
@13:13:04.67 Adding MM1
@13:13:04.73 AS.seek 0.524ml
@13:13:23.34 delay 25 seconds
@13:13:48.36 ARV.dial :PP1
@13:13:48.76 Adding PP1
@13:13:48.77 delay 2 seconds
@13:13:50.84 AS.seek 0.53ml
@13:13:57.50 delay 10 seconds
@13:14:07.53 ARV.dial :air1
@13:14:07.94 adding ARV air
@13:14:07.95 delay 2 seconds
@13:14:10.02 AS.seek 0.536ml
@13:14:16.22 delay 5 seconds
@13:14:21.24 ARV.dial :carrier
@13:14:21.65 delay 7 seconds
@13:14:28.73 AS.seek 0.594ml
@13:15:27.15 delay 5 seconds
@13:15:32.17 ATV.dial :ASV
@13:15:32.62 delay 2 seconds
@13:15:34.69 AS.seek 0.6ml
@13:15:41.33 delay 4 seconds
@13:15:45.35 ATV.dial ARV
@13:15:45.94 delay 2 seconds
@13:15:47.96 ARV.dial :carrier
@13:15:47.97 delay 2 seconds
@13:15:50.04 AS.seek 0.65ml
@13:16:40.65 delay 4 seconds
@13:16:44.67 ATV.dial PCR
@13:16:45.29 ASV.dial :waste1
@13:16:45.30 ARV.dial :air1
@13:16:45.70 Positioning Slug by 0.324ml
@13:16:46.19 AS.seek 0.326ml
@13:22:12.62 delay 5 seconds
@13:22:17.65 ATV.dial ARV
@13:22:18.44 ASV.dial :PCRlock
@13:22:18.83 delay 5 seconds
@13:22:23.85 Running AOPP1 Profile60CIQ1 thermocycling
@13:22:23.86 <PCR> PCR.program Profile60CIQ1
@13:22:23.97 Profile60CIQ1 should complete in < 1:56:15
@13:22:23.98 PCR.run 2:30:18.75
@15:01:43.85 PCR.off
@15:01:44.39 <MFBPCR> Running AOPP1 MeltProfile60 thermocycling
@15:01:44.41 <PCR> PCR.program MeltProfile60
@15:01:44.49 MeltProfile60 should complete in < 11:55
@15:01:44.50 PCR.run 19:53.75
@15:09:34.45 PCR.off
@15:09:35.37 <MFBPCR> PCR for AOPP1 completed -- closed /var/log/esp/AOPP121apr2613h.pcr
@15:09:35.42 Cleaning PCR
@15:09:35.43 AFV.select :carrierSolution
@15:09:35.53 delay 2 seconds
@15:09:37.56 AS.reconfigure ASconfig
@15:09:37.60 AS.seek 0.5ml
@15:09:47.93 delay 1 second
@15:09:48.95 AFV.select :holdingCoil
@15:09:49.05 ASV.dial :waste1
@15:09:49.45 ATV.dial :air3
@15:09:50.81 AS.reconfigure ASslow1
@15:09:50.86 AS.seek 0.51ml
@15:09:53.27 delay 2 seconds
@15:09:55.29 ATV.dial :bleach
@15:09:55.92 AS.seek 0.81ml
@15:10:56.51 delay 3 seconds
@15:10:59.53 ATV.dial :air3
@15:11:00.11 delay 3 seconds
@15:11:03.18 AS.seek 0.835ml
@15:11:08.56 delay 3 seconds
@15:11:11.58 ATV.dial :bleach
@15:11:12.20 AS.seek 0.82ml
@15:11:15.56 delay 3 seconds
@15:11:18.58 ARV.dial :waste
@15:11:19.21 delay 3 seconds
@15:11:22.23 ATV.dial :ARV
@15:11:23.25 AS.reconfigure ASconfig
@15:11:23.29 AS.seek 0.47ml
@15:11:43.71 AFV.select :carrierSolution
@15:11:43.81 delay 2 seconds
@15:11:45.84 AS.seek 1ml
@15:12:16.57 delay 1 second
@15:12:17.59 AFV.select :holdingCoil
@15:12:17.69 delay 1 second
@15:12:18.72 AS.reconfigure ASfast
@15:12:18.76 AS.seek 0ml
@15:12:46.11 AFV.select :carrierSolution
@15:12:46.21 delay 2 seconds
@15:12:48.23 AS.reconfigure ASconfig
@15:12:48.28 AS.seek 1ml
@15:13:46.04 delay 1 second
@15:13:47.06 AFV.select :holdingCoil
@15:13:47.16 delay 1 second
@15:13:48.19 AS.reconfigure ASfast
@15:13:48.23 AS.seek 0ml
@15:14:15.54 AFV.select :carrierSolution
@15:14:15.64 delay 2 seconds
@15:14:17.67 AS.reconfigure ASconfig
@15:14:17.71 AS.seek 1ml
@15:15:15.48 delay 1 second
@15:15:16.50 AFV.select :holdingCoil
@15:15:16.60 delay 1 second
@15:15:17.63 AS.reconfigure ASfast
@15:15:17.67 AS.seek 0ml
@15:15:45.00 ARV.dial between carrier and air1
@15:15:45.51 ATV.dial :air3
@15:15:46.84 AS.reconfigure ASslow1
@15:15:46.89 AS.seek 0.01ml
@15:15:49.20 delay 2 seconds
@15:15:51.22 ATV.dial :bleach
@15:15:51.84 AS.seek 0.81ml
@15:18:32.96 delay 3 seconds
@15:18:35.98 ATV.dial :air3
@15:18:36.56 delay 3 seconds
@15:18:39.63 AS.seek 0.835ml
@15:18:44.91 delay 3 seconds
@15:18:47.93 ATV.dial :bleach
@15:18:48.56 AS.seek 0.82ml
@15:18:51.86 delay 3 seconds
@15:18:54.88 ATV.dial PCR
@15:18:55.28 AS.reconfigure ASconfig
@15:18:55.33 AS.seek 0ml
@15:19:42.73 PCR.program :bleachProfile
@15:19:42.78 PCR program should complete in < 2:45
@15:19:42.79 PCR.run 10:00
@15:22:38.98 PCR.off
@15:22:39.01 AFV.select :carrierSolution
@15:22:39.11 delay 2 seconds
@15:22:41.14 AS.seek 1ml
@15:23:38.90 delay 1 second
@15:23:39.92 AFV.select :holdingCoil
@15:23:40.02 delay 1 second
@15:23:41.05 AS.reconfigure ASfast
@15:23:41.09 AS.seek 0ml
@15:24:08.42 delay 3 seconds
@15:24:11.44 AFV.select :carrierSolution
@15:24:11.54 delay 2 seconds
@15:24:13.56 AS.reconfigure ASconfig
@15:24:13.61 AS.seek 1ml
@15:25:11.37 delay 1 second
@15:25:12.39 AFV.select :holdingCoil
@15:25:12.49 delay 1 second
@15:25:13.52 AS.reconfigure ASfast
@15:25:13.56 AS.seek 0ml
@15:25:40.90 delay 3 seconds
@15:25:43.92 AFV.select :carrierSolution
@15:25:44.02 delay 2 seconds
@15:25:46.05 AS.reconfigure ASconfig
@15:25:46.10 AS.seek 1ml
@15:26:43.87 delay 1 second
@15:26:44.89 AFV.select :holdingCoil
@15:26:44.99 delay 1 second
@15:26:46.02 AS.reconfigure ASfast
@15:26:46.06 AS.seek 0ml
@15:27:13.39 delay 3 seconds
@15:27:16.41 AFV.select :carrierSolution
@15:27:16.51 delay 2 seconds
@15:27:18.53 AS.reconfigure ASconfig
@15:27:18.58 AS.seek 1ml
@15:28:16.34 delay 1 second
@15:28:17.36 AFV.select :holdingCoil
@15:28:17.46 delay 1 second
@15:28:18.49 AS.reconfigure ASfast
@15:28:18.53 AS.seek 0ml
@15:28:45.87 delay 3 seconds
@15:28:48.89 AFV.select :carrierSolution
@15:28:48.99 delay 2 seconds
@15:28:51.01 AS.reconfigure ASconfig
@15:28:51.06 AS.seek 1ml
@15:29:48.82 delay 1 second
@15:29:49.84 AFV.select :holdingCoil
@15:29:49.94 delay 1 second
@15:29:50.97 AS.reconfigure ASnorm
@15:29:51.01 AS.seek 0ml
@15:30:41.70 Setting up PCR for AARPP4 -- writing /var/log/esp/AARPP421apr2615h.pcr
@15:30:41.72 Building PCR Slug from ASV with MM2 mix, PP4 primer
@15:30:41.73 Priming with carrier
@15:30:41.74 ATV.dial ARV
@15:30:42.37 ARV.dial :carrier
@15:30:42.71 AS.seek 0.5ml
@15:31:08.25 AFV.select :carrierSolution
@15:31:08.35 delay 2 seconds
@15:31:10.38 AS.reconfigure ASconfig
@15:31:10.42 AS.seek 1ml
@15:31:39.42 delay 1 second
@15:31:40.44 AFV.select :holdingCoil
@15:31:40.54 delay 1 second
@15:31:41.56 ATV.dial :waste
@15:31:43.32 AS.reconfigure ASnorm
@15:31:43.37 AS.seek 0ml
@15:32:34.04 AFV.select :carrierSolution
@15:32:34.14 delay 2 seconds
@15:32:36.17 AS.reconfigure ASconfig
@15:32:36.21 AS.seek 0.5ml
@15:33:05.23 delay 1 second
@15:33:06.25 AFV.select :holdingCoil
@15:33:06.35 Building Slug
@15:33:06.36 ATV.dial :ARV
@15:33:08.09 delay 2 seconds
@15:33:10.11 ARV.dial :air1
@15:33:10.50 delay 2 seconds
@15:33:12.57 AS.reconfigure ASslow2
@15:33:12.61 AS.seek 0.506ml
@15:33:19.00 delay 2 seconds
@15:33:21.02 ARV.dial :MM2
@15:33:22.60 delay 2 seconds
@15:33:24.62 Adding MM2
@15:33:24.68 AS.seek 0.524ml
@15:33:43.73 delay 25 seconds
@15:34:08.76 ARV.dial :PP4
@15:34:09.18 Adding PP4
@15:34:09.19 delay 2 seconds
@15:34:11.26 AS.seek 0.53ml
@15:34:17.70 delay 10 seconds
@15:34:27.73 ARV.dial :air3
@15:34:28.12 adding ARV air
@15:34:28.13 delay 2 seconds
@15:34:30.20 AS.seek 0.536ml
@15:34:36.75 delay 5 seconds
@15:34:41.77 ARV.dial :carrier
@15:34:43.09 delay 7 seconds
@15:34:50.16 AS.seek 0.594ml
@15:35:48.76 delay 5 seconds
@15:35:53.78 ATV.dial :ASV
@15:35:54.20 delay 2 seconds
@15:35:56.27 AS.seek 0.6ml
@15:36:02.53 delay 4 seconds
@15:36:06.55 ATV.dial ARV
@15:36:07.10 delay 2 seconds
@15:36:09.12 ARV.dial :carrier
@15:36:09.13 delay 2 seconds
@15:36:11.20 AS.seek 0.65ml
@15:37:02.29 delay 4 seconds
@15:37:06.31 ATV.dial PCR
@15:37:06.95 ASV.dial :waste1
@15:37:06.96 ARV.dial :air1
@15:37:07.38 Positioning Slug by 0.324ml
@15:37:07.45 AS.seek 0.326ml
@15:42:33.79 delay 5 seconds
@15:42:38.81 ATV.dial ARV
@15:42:39.62 ASV.dial :PCRlock
@15:42:40.01 delay 5 seconds
@15:42:45.03 Running AARPP4 Profile58CIQ2 thermocycling
@15:42:45.04 <PCR> PCR.program Profile58CIQ2
@15:42:45.14 Profile58CIQ2 should complete in < 1:36:15
@15:42:45.15 PCR.run 2:05:18.75
@17:04:24.16 PCR.off
@17:04:24.71 <MFBPCR> Running AARPP4 MeltProfile58 thermocycling
@17:04:24.72 <PCR> PCR.program MeltProfile58
@17:04:24.80 MeltProfile58 should complete in < 12:33
@17:04:24.81 PCR.run 20:41.25
@17:12:24.92 PCR.off
@17:12:25.88 <MFBPCR> PCR for AARPP4 completed -- closed /var/log/esp/AARPP421apr2615h.pcr
@17:12:25.94 Cleaning PCR
@17:12:25.95 AFV.select :carrierSolution
@17:12:26.05 delay 2 seconds
@17:12:28.08 AS.reconfigure ASconfig
@17:12:28.12 AS.seek 0.5ml
@17:12:38.40 delay 1 second
@17:12:39.42 AFV.select :holdingCoil
@17:12:39.52 ASV.dial :waste1
@17:12:39.91 ATV.dial :air3
@17:12:41.29 AS.reconfigure ASslow1
@17:12:41.34 AS.seek 0.51ml
@17:12:43.63 delay 2 seconds
@17:12:45.65 ATV.dial :bleach
@17:12:46.28 AS.seek 0.81ml
@17:13:46.85 delay 3 seconds
@17:13:49.87 ATV.dial :air3
@17:13:50.45 delay 3 seconds
@17:13:53.52 AS.seek 0.835ml
@17:13:58.91 delay 3 seconds
@17:14:01.93 ATV.dial :bleach
@17:14:02.56 AS.seek 0.82ml
@17:14:05.95 delay 3 seconds
@17:14:08.97 ARV.dial :waste
@17:14:09.60 delay 3 seconds
@17:14:12.62 ATV.dial :ARV
@17:14:13.56 AS.reconfigure ASconfig
@17:14:13.60 AS.seek 0.47ml
@17:14:34.01 AFV.select :carrierSolution
@17:14:34.11 delay 2 seconds
@17:14:36.14 AS.seek 1ml
@17:15:07.29 delay 1 second
@17:15:08.32 AFV.select :holdingCoil
@17:15:08.42 delay 1 second
@17:15:09.45 AS.reconfigure ASfast
@17:15:09.49 AS.seek 0ml
@17:15:36.82 AFV.select :carrierSolution
@17:15:36.92 delay 2 seconds
@17:15:38.95 AS.reconfigure ASconfig
@17:15:38.99 AS.seek 1ml
@17:16:36.74 delay 1 second
@17:16:37.76 AFV.select :holdingCoil
@17:16:37.86 delay 1 second
@17:16:38.89 AS.reconfigure ASfast
@17:16:38.93 AS.seek 0ml
@17:17:06.24 AFV.select :carrierSolution
@17:17:06.34 delay 2 seconds
@17:17:08.36 AS.reconfigure ASconfig
@17:17:08.41 AS.seek 1ml
@17:18:06.15 delay 1 second
@17:18:07.17 AFV.select :holdingCoil
@17:18:07.27 delay 1 second
@17:18:08.30 AS.reconfigure ASfast
@17:18:08.34 AS.seek 0ml
@17:18:35.66 ARV.dial between carrier and air1
@17:18:36.18 ATV.dial :air3
@17:18:37.53 AS.reconfigure ASslow1
@17:18:37.58 AS.seek 0.01ml
@17:18:39.98 delay 2 seconds
@17:18:42.00 ATV.dial :bleach
@17:18:42.62 AS.seek 0.81ml
@17:21:23.74 delay 3 seconds
@17:21:26.76 ATV.dial :air3
@17:21:27.34 delay 3 seconds
@17:21:30.41 AS.seek 0.835ml
@17:21:35.68 delay 3 seconds
@17:21:38.70 ATV.dial :bleach
@17:21:39.32 AS.seek 0.82ml
@17:21:42.62 delay 3 seconds
@17:21:45.64 ATV.dial PCR
@17:21:46.06 AS.reconfigure ASconfig
@17:21:46.11 AS.seek 0ml
@17:22:33.52 PCR.program :bleachProfile
@17:22:33.57 PCR program should complete in < 2:45
@17:22:33.58 PCR.run 10:00
@17:25:29.35 PCR.off
@17:25:29.38 AFV.select :carrierSolution
@17:25:29.48 delay 2 seconds
@17:25:31.51 AS.seek 1ml
@17:26:29.26 delay 1 second
@17:26:30.30 AFV.select :holdingCoil
@17:26:30.40 delay 1 second
@17:26:31.43 AS.reconfigure ASfast
@17:26:31.47 AS.seek 0ml
@17:26:58.80 delay 3 seconds
@17:27:01.82 AFV.select :carrierSolution
@17:27:01.92 delay 2 seconds
@17:27:03.94 AS.reconfigure ASconfig
@17:27:03.99 AS.seek 1ml
@17:28:01.77 delay 1 second
@17:28:02.79 AFV.select :holdingCoil
@17:28:02.89 delay 1 second
@17:28:03.91 AS.reconfigure ASfast
@17:28:03.96 AS.seek 0ml
@17:28:31.27 delay 3 seconds
@17:28:34.29 AFV.select :carrierSolution
@17:28:34.39 delay 2 seconds
@17:28:36.77 AS.reconfigure ASconfig
@17:28:36.87 AS.seek 1ml
@17:29:34.60 delay 1 second
@17:29:35.62 AFV.select :holdingCoil
@17:29:35.72 delay 1 second
@17:29:36.75 AS.reconfigure ASfast
@17:29:36.79 AS.seek 0ml
@17:30:04.13 delay 3 seconds
@17:30:07.15 AFV.select :carrierSolution
@17:30:07.25 delay 2 seconds
@17:30:09.27 AS.reconfigure ASconfig
@17:30:09.33 AS.seek 1ml
@17:31:07.12 delay 1 second
@17:31:08.14 AFV.select :holdingCoil
@17:31:08.24 delay 1 second
@17:31:09.27 AS.reconfigure ASfast
@17:31:09.31 AS.seek 0ml
@17:31:36.63 delay 3 seconds
@17:31:39.65 AFV.select :carrierSolution
@17:31:39.75 delay 2 seconds
@17:31:41.77 AS.reconfigure ASconfig
@17:31:41.83 AS.seek 1ml
@17:32:39.57 delay 1 second
@17:32:40.59 AFV.select :holdingCoil
@17:32:40.69 delay 1 second
@17:32:41.72 AS.reconfigure ASnorm
@17:32:41.76 AS.seek 0ml
@17:33:32.45 Setting up PCR for AALPP3 -- writing /var/log/esp/AALPP321apr2617h.pcr
@17:33:32.47 Building PCR Slug from ASV with MM2 mix, PP3 primer
@17:33:32.48 Priming with carrier
@17:33:32.49 ATV.dial ARV
@17:33:33.13 ARV.dial :carrier
@17:33:33.47 AS.seek 0.5ml
@17:33:59.04 AFV.select :carrierSolution
@17:33:59.14 delay 2 seconds
@17:34:01.17 AS.reconfigure ASconfig
@17:34:01.21 AS.seek 1ml
@17:34:30.21 delay 1 second
@17:34:31.23 AFV.select :holdingCoil
@17:34:31.33 delay 1 second
@17:34:32.35 ATV.dial :waste
@17:34:34.12 AS.reconfigure ASnorm
@17:34:34.17 AS.seek 0ml
@17:35:24.84 AFV.select :carrierSolution
@17:35:24.94 delay 2 seconds
@17:35:26.97 AS.reconfigure ASconfig
@17:35:27.01 AS.seek 0.5ml
@17:35:56.07 delay 1 second
@17:35:57.09 AFV.select :holdingCoil
@17:35:57.19 Building Slug
@17:35:57.20 ATV.dial :ARV
@17:35:58.91 delay 2 seconds
@17:36:00.93 ARV.dial :air1
@17:36:01.33 delay 2 seconds
@17:36:03.40 AS.reconfigure ASslow2
@17:36:03.44 AS.seek 0.506ml
@17:36:09.82 delay 2 seconds
@17:36:11.84 ARV.dial :MM2
@17:36:13.43 delay 2 seconds
@17:36:15.46 Adding MM2
@17:36:15.52 AS.seek 0.524ml
@17:36:34.18 delay 25 seconds
@17:36:59.20 ARV.dial :PP3
@17:36:59.60 Adding PP3
@17:36:59.61 delay 2 seconds
@17:37:01.68 AS.seek 0.53ml
@17:37:08.07 delay 10 seconds
@17:37:18.10 ARV.dial :air2
@17:37:18.51 adding ARV air
@17:37:18.52 delay 2 seconds
@17:37:20.59 AS.seek 0.536ml
@17:37:27.16 delay 5 seconds
@17:37:32.18 ARV.dial :carrier
@17:37:33.55 delay 7 seconds
@17:37:40.62 AS.seek 0.594ml
@17:38:39.23 delay 5 seconds
@17:38:44.25 ATV.dial :ASV
@17:38:44.66 delay 2 seconds
@17:38:46.73 AS.seek 0.6ml
@17:38:53.38 delay 4 seconds
@17:38:57.40 ATV.dial ARV
@17:38:57.96 delay 2 seconds
@17:38:59.98 ARV.dial :carrier
@17:38:59.99 delay 2 seconds
@17:39:02.06 AS.seek 0.65ml
@17:39:52.51 delay 4 seconds
@17:39:56.53 ATV.dial PCR
@17:39:57.16 ASV.dial :waste1
@17:39:57.17 ARV.dial :air1
@17:39:57.58 Positioning Slug by 0.324ml
@17:39:57.65 AS.seek 0.326ml
@17:45:23.94 delay 5 seconds
@17:45:28.96 ATV.dial ARV
@17:45:29.77 ASV.dial :PCRlock
@17:45:30.16 delay 5 seconds
@17:45:35.18 Running AALPP3 Profile58CIQ2 thermocycling
@17:45:35.20 <PCR> PCR.program Profile58CIQ2
@17:45:35.30 Profile58CIQ2 should complete in < 1:36:15
@17:45:35.31 PCR.run 2:05:18.75
@19:06:58.06 PCR.off
@19:06:58.60 <MFBPCR> Running AALPP3 MeltProfile58 thermocycling
@19:06:58.62 <PCR> PCR.program MeltProfile58
@19:06:58.70 MeltProfile58 should complete in < 12:33
@19:06:58.71 PCR.run 20:41.25
@19:14:55.91 PCR.off
@19:14:56.88 <MFBPCR> PCR for AALPP3 completed -- closed /var/log/esp/AALPP321apr2617h.pcr
@19:14:56.93 Cleaning PCR
@19:14:56.94 AFV.select :carrierSolution
@19:14:57.04 delay 2 seconds
@19:14:59.07 AS.reconfigure ASconfig
@19:14:59.11 AS.seek 0.5ml
@19:15:09.41 delay 1 second
@19:15:10.43 AFV.select :holdingCoil
@19:15:10.53 ASV.dial :waste1
@19:15:10.93 ATV.dial :air3
@19:15:12.26 AS.reconfigure ASslow1
@19:15:12.31 AS.seek 0.51ml
@19:15:14.58 delay 2 seconds
@19:15:16.60 ATV.dial :bleach
@19:15:17.23 AS.seek 0.81ml
@19:16:17.96 delay 3 seconds
@19:16:20.98 ATV.dial :air3
@19:16:21.57 delay 3 seconds
@19:16:24.64 AS.seek 0.835ml
@19:16:30.01 delay 3 seconds
@19:16:33.03 ATV.dial :bleach
@19:16:33.65 AS.seek 0.82ml
@19:16:36.97 delay 3 seconds
@19:16:39.99 ARV.dial :waste
@19:16:40.63 delay 3 seconds
@19:16:43.65 ATV.dial :ARV
@19:16:44.57 AS.reconfigure ASconfig
@19:16:44.61 AS.seek 0.47ml
@19:17:05.00 AFV.select :carrierSolution
@19:17:05.10 delay 2 seconds
@19:17:07.13 AS.seek 1ml
@19:17:37.85 delay 1 second
@19:17:38.87 AFV.select :holdingCoil
@19:17:38.97 delay 1 second
@19:17:40.00 AS.reconfigure ASfast
@19:17:40.04 AS.seek 0ml
@19:18:07.36 AFV.select :carrierSolution
@19:18:07.46 delay 2 seconds
@19:18:09.48 AS.reconfigure ASconfig
@19:18:09.53 AS.seek 1ml
@19:19:07.32 delay 1 second
@19:19:08.34 AFV.select :holdingCoil
@19:19:08.44 delay 1 second
@19:19:09.47 AS.reconfigure ASfast
@19:19:09.51 AS.seek 0ml
@19:19:36.83 AFV.select :carrierSolution
@19:19:36.93 delay 2 seconds
@19:19:38.95 AS.reconfigure ASconfig
@19:19:39.00 AS.seek 1ml
@19:20:36.79 delay 1 second
@19:20:37.81 AFV.select :holdingCoil
@19:20:37.91 delay 1 second
@19:20:38.94 AS.reconfigure ASfast
@19:20:38.98 AS.seek 0ml
@19:21:06.30 ARV.dial between carrier and air1
@19:21:06.82 ATV.dial :air3
@19:21:08.15 AS.reconfigure ASslow1
@19:21:08.20 AS.seek 0.01ml
@19:21:10.61 delay 2 seconds
@19:21:12.63 ATV.dial :bleach
@19:21:13.26 AS.seek 0.81ml
@19:23:54.47 delay 3 seconds
@19:23:57.49 ATV.dial :air3
@19:23:58.07 delay 3 seconds
@19:24:01.14 AS.seek 0.835ml
@19:24:06.41 delay 3 seconds
@19:24:09.43 ATV.dial :bleach
@19:24:10.05 AS.seek 0.82ml
@19:24:13.35 delay 3 seconds
@19:24:16.37 ATV.dial PCR
@19:24:16.77 AS.reconfigure ASconfig
@19:24:16.82 AS.seek 0ml
@19:25:04.22 PCR.program :bleachProfile
@19:25:04.28 PCR program should complete in < 2:45
@19:25:04.29 PCR.run 10:00
@19:28:00.47 PCR.off
@19:28:00.50 AFV.select :carrierSolution
@19:28:00.60 delay 2 seconds
@19:28:02.63 AS.seek 1ml
@19:29:00.38 delay 1 second
@19:29:01.40 AFV.select :holdingCoil
@19:29:01.50 delay 1 second
@19:29:02.53 AS.reconfigure ASfast
@19:29:02.57 AS.seek 0ml
@19:29:29.91 delay 3 seconds
@19:29:32.93 AFV.select :carrierSolution
@19:29:33.03 delay 2 seconds
@19:29:35.05 AS.reconfigure ASconfig
@19:29:35.11 AS.seek 1ml
@19:30:32.90 delay 1 second
@19:30:33.92 AFV.select :holdingCoil
@19:30:34.02 delay 1 second
@19:30:35.05 AS.reconfigure ASfast
@19:30:35.09 AS.seek 0ml
@19:31:02.43 delay 3 seconds
@19:31:05.45 AFV.select :carrierSolution
@19:31:05.55 delay 2 seconds
@19:31:07.57 AS.reconfigure ASconfig
@19:31:07.62 AS.seek 1ml
@19:32:05.36 delay 1 second
@19:32:06.38 AFV.select :holdingCoil
@19:32:06.48 delay 1 second
@19:32:07.51 AS.reconfigure ASfast
@19:32:07.55 AS.seek 0ml
@19:32:34.91 delay 3 seconds
@19:32:37.93 AFV.select :carrierSolution
@19:32:38.03 delay 2 seconds
@19:32:40.05 AS.reconfigure ASconfig
@19:32:40.10 AS.seek 1ml
@19:33:37.85 delay 1 second
@19:33:38.87 AFV.select :holdingCoil
@19:33:38.97 delay 1 second
@19:33:39.99 AS.reconfigure ASfast
@19:33:40.04 AS.seek 0ml
@19:34:07.38 delay 3 seconds
@19:34:10.40 AFV.select :carrierSolution
@19:34:10.50 delay 2 seconds
@19:34:12.52 AS.reconfigure ASconfig
@19:34:12.57 AS.seek 1ml
@19:35:10.35 delay 1 second
@19:35:11.37 AFV.select :holdingCoil
@19:35:11.47 delay 1 second
@19:35:12.50 AS.reconfigure ASnorm
@19:35:12.54 AS.seek 0ml
@19:36:03.23 Setting up PCR for ACOPP5 -- writing /var/log/esp/ACOPP521apr2619h.pcr
@19:36:03.25 Building PCR Slug from ASV with MM3 mix, PP5 primer
@19:36:03.26 Priming with carrier
@19:36:03.27 ATV.dial ARV
@19:36:03.91 ARV.dial :carrier
@19:36:04.25 AS.seek 0.5ml
@19:36:29.75 AFV.select :carrierSolution
@19:36:29.85 delay 2 seconds
@19:36:31.88 AS.reconfigure ASconfig
@19:36:31.92 AS.seek 1ml
@19:37:00.93 delay 1 second
@19:37:01.95 AFV.select :holdingCoil
@19:37:02.05 delay 1 second
@19:37:03.07 ATV.dial :waste
@19:37:04.83 AS.reconfigure ASnorm
@19:37:04.88 AS.seek 0ml
@19:37:55.55 AFV.select :carrierSolution
@19:37:55.65 delay 2 seconds
@19:37:57.68 AS.reconfigure ASconfig
@19:37:57.72 AS.seek 0.5ml
@19:38:26.77 delay 1 second
@19:38:27.79 AFV.select :holdingCoil
@19:38:27.89 Building Slug
@19:38:27.90 ATV.dial :ARV
@19:38:29.61 delay 2 seconds
@19:38:31.63 ARV.dial :air1
@19:38:32.05 delay 2 seconds
@19:38:34.12 AS.reconfigure ASslow2
@19:38:34.16 AS.seek 0.506ml
@19:38:40.91 delay 2 seconds
@19:38:42.93 ARV.dial :MM3
@19:38:44.05 delay 2 seconds
@19:38:46.07 Adding MM3
@19:38:46.14 AS.seek 0.524ml
@19:39:05.16 delay 25 seconds
@19:39:30.18 ARV.dial :PP5
@19:39:30.58 Adding PP5
@19:39:30.59 delay 2 seconds
@19:39:32.66 AS.seek 0.53ml
@19:39:38.94 delay 10 seconds
@19:39:48.97 ARV.dial :air3
@19:39:49.38 adding ARV air
@19:39:49.39 delay 2 seconds
@19:39:51.46 AS.seek 0.536ml
@19:39:57.94 delay 5 seconds
@19:40:02.96 ARV.dial :carrier
@19:40:04.32 delay 7 seconds
@19:40:11.80 AS.seek 0.594ml
@19:41:10.61 delay 5 seconds
@19:41:15.63 ATV.dial :ASV
@19:41:16.05 delay 2 seconds
@19:41:18.12 AS.seek 0.6ml
@19:41:24.57 delay 4 seconds
@19:41:28.59 ATV.dial ARV
@19:41:29.15 delay 2 seconds
@19:41:31.17 ARV.dial :carrier
@19:41:31.18 delay 2 seconds
@19:41:33.25 AS.seek 0.65ml
@19:42:23.93 delay 4 seconds
@19:42:27.95 ATV.dial PCR
@19:42:28.58 ASV.dial :waste1
@19:42:28.59 ARV.dial :air1
@19:42:28.98 Positioning Slug by 0.324ml
@19:42:29.05 AS.seek 0.326ml
@19:47:55.14 delay 5 seconds
@19:48:00.16 ATV.dial ARV
@19:48:00.97 ASV.dial :PCRlock
@19:48:01.36 delay 5 seconds
@19:48:06.38 Running ACOPP5 ProfileIPc thermocycling
@19:48:06.39 <PCR> PCR.program ProfileIPc
@19:48:06.49 ProfileIPc should complete in < 1:26:35
@19:48:06.50 PCR.run 1:53:13.75
@20:57:11.20 PCR.off
@20:57:11.75 <MFBPCR> PCR for ACOPP5 completed -- closed /var/log/esp/ACOPP521apr2619h.pcr
@20:57:11.81 Cleaning PCR
@20:57:11.82 AFV.select :carrierSolution
@20:57:11.92 delay 2 seconds
@20:57:13.95 AS.reconfigure ASconfig
@20:57:13.99 AS.seek 0.5ml
@20:57:24.25 delay 1 second
@20:57:25.27 AFV.select :holdingCoil
@20:57:25.37 ASV.dial :waste1
@20:57:25.77 ATV.dial :air3
@20:57:27.10 AS.reconfigure ASslow1
@20:57:27.15 AS.seek 0.51ml
@20:57:29.43 delay 2 seconds
@20:57:31.44 ATV.dial :bleach
@20:57:32.07 AS.seek 0.81ml
@20:58:32.66 delay 3 seconds
@20:58:35.68 ATV.dial :air3
@20:58:36.25 delay 3 seconds
@20:58:39.32 AS.seek 0.835ml
@20:58:44.60 delay 3 seconds
@20:58:47.62 ATV.dial :bleach
@20:58:48.24 AS.seek 0.82ml
@20:58:51.63 delay 3 seconds
@20:58:54.65 ARV.dial :waste
@20:58:55.27 delay 3 seconds
@20:58:58.29 ATV.dial :ARV
@20:58:59.21 AS.reconfigure ASconfig
@20:58:59.26 AS.seek 0.47ml
@20:59:19.64 AFV.select :carrierSolution
@20:59:19.74 delay 2 seconds
@20:59:21.77 AS.seek 1ml
@20:59:52.50 delay 1 second
@20:59:53.52 AFV.select :holdingCoil
@20:59:53.62 delay 1 second
@20:59:54.65 AS.reconfigure ASfast
@20:59:54.69 AS.seek 0ml
@21:00:22.02 AFV.select :carrierSolution
@21:00:22.12 delay 2 seconds
@21:00:24.14 AS.reconfigure ASconfig
@21:00:24.19 AS.seek 1ml
@21:01:21.92 delay 1 second
@21:01:22.94 AFV.select :holdingCoil
@21:01:23.04 delay 1 second
@21:01:24.07 AS.reconfigure ASfast
@21:01:24.11 AS.seek 0ml
@21:01:51.44 AFV.select :carrierSolution
@21:01:51.54 delay 2 seconds
@21:01:53.56 AS.reconfigure ASconfig
@21:01:53.61 AS.seek 1ml
@21:02:51.38 delay 1 second
@21:02:52.40 AFV.select :holdingCoil
@21:02:52.50 delay 1 second
@21:02:53.53 AS.reconfigure ASfast
@21:02:53.57 AS.seek 0ml
@21:03:20.91 ARV.dial between carrier and air1
@21:03:21.42 ATV.dial :air3
@21:03:22.76 AS.reconfigure ASslow1
@21:03:22.80 AS.seek 0.01ml
@21:03:25.14 delay 2 seconds
@21:03:27.16 ATV.dial :bleach
@21:03:27.79 AS.seek 0.81ml
@21:06:08.94 delay 3 seconds
@21:06:11.96 ATV.dial :air3
@21:06:12.53 delay 3 seconds
@21:06:15.60 AS.seek 0.835ml
@21:06:20.86 delay 3 seconds
@21:06:23.88 ATV.dial :bleach
@21:06:24.51 AS.seek 0.82ml
@21:06:27.94 delay 3 seconds
@21:06:30.96 ATV.dial PCR
@21:06:31.36 AS.reconfigure ASconfig
@21:06:31.40 AS.seek 0ml
@21:07:18.83 PCR.program :bleachProfile
@21:07:18.88 PCR program should complete in < 2:45
@21:07:18.89 PCR.run 10:00
@21:10:15.08 PCR.off
@21:10:15.11 AFV.select :carrierSolution
@21:10:15.21 delay 2 seconds
@21:10:17.24 AS.seek 1ml
@21:11:15.02 delay 1 second
@21:11:16.04 AFV.select :holdingCoil
@21:11:16.14 delay 1 second
@21:11:17.17 AS.reconfigure ASfast
@21:11:17.21 AS.seek 0ml
@21:11:44.53 delay 3 seconds
@21:11:47.55 AFV.select :carrierSolution
@21:11:47.65 delay 2 seconds
@21:11:49.67 AS.reconfigure ASconfig
@21:11:49.72 AS.seek 1ml
@21:12:47.46 delay 1 second
@21:12:48.48 AFV.select :holdingCoil
@21:12:48.58 delay 1 second
@21:12:49.61 AS.reconfigure ASfast
@21:12:49.65 AS.seek 0ml
@21:13:17.00 delay 3 seconds
@21:13:20.02 AFV.select :carrierSolution
@21:13:20.12 delay 2 seconds
@21:13:22.15 AS.reconfigure ASconfig
@21:13:22.19 AS.seek 1ml
@21:14:19.92 delay 1 second
@21:14:20.94 AFV.select :holdingCoil
@21:14:21.04 delay 1 second
@21:14:22.06 AS.reconfigure ASfast
@21:14:22.11 AS.seek 0ml
@21:14:49.42 delay 3 seconds
@21:14:52.44 AFV.select :carrierSolution
@21:14:52.54 delay 2 seconds
@21:14:54.56 AS.reconfigure ASconfig
@21:14:54.61 AS.seek 1ml
@21:15:52.36 delay 1 second
@21:15:53.38 AFV.select :holdingCoil
@21:15:53.48 delay 1 second
@21:15:54.51 AS.reconfigure ASfast
@21:15:54.55 AS.seek 0ml
@21:16:21.88 delay 3 seconds
@21:16:24.90 AFV.select :carrierSolution
@21:16:25.00 delay 2 seconds
@21:16:27.02 AS.reconfigure ASconfig
@21:16:27.08 AS.seek 1ml
@21:17:24.86 delay 1 second
@21:17:25.88 AFV.select :holdingCoil
@21:17:25.98 delay 1 second
@21:17:27.01 AS.reconfigure ASnorm
@21:17:27.05 AS.seek 0ml
@21:18:17.76 Setting up PCR for AIPc -- writing /var/log/esp/AIPc21apr2621h.pcr
@21:18:17.78 Building PCR Slug from ASV with MM3 mix, PP6 primer
@21:18:17.79 Priming with carrier
@21:18:17.80 ATV.dial ARV
@21:18:18.44 ARV.dial :carrier
@21:18:18.78 AS.seek 0.5ml
@21:18:44.35 AFV.select :carrierSolution
@21:18:44.45 delay 2 seconds
@21:18:46.48 AS.reconfigure ASconfig
@21:18:46.52 AS.seek 1ml
@21:19:15.52 delay 1 second
@21:19:16.54 AFV.select :holdingCoil
@21:19:16.64 delay 1 second
@21:19:17.66 ATV.dial :waste
@21:19:19.41 AS.reconfigure ASnorm
@21:19:19.46 AS.seek 0ml
@21:20:10.13 AFV.select :carrierSolution
@21:20:10.23 delay 2 seconds
@21:20:12.26 AS.reconfigure ASconfig
@21:20:12.30 AS.seek 0.5ml
@21:20:41.35 delay 1 second
@21:20:42.37 AFV.select :holdingCoil
@21:20:42.47 Building Slug
@21:20:42.48 ATV.dial :ARV
@21:20:44.21 delay 2 seconds
@21:20:46.23 ARV.dial :air1
@21:20:46.61 delay 2 seconds
@21:20:48.68 AS.reconfigure ASslow2
@21:20:48.72 AS.seek 0.506ml
@21:20:55.14 delay 2 seconds
@21:20:57.16 ARV.dial :MM3
@21:20:58.25 delay 2 seconds
@21:21:00.27 Adding MM3
@21:21:00.74 AS.seek 0.524ml
@21:21:18.97 delay 25 seconds
@21:21:43.99 ARV.dial :PP6
@21:21:44.38 Adding PP6
@21:21:44.39 delay 2 seconds
@21:21:46.46 AS.seek 0.53ml
@21:21:52.64 delay 10 seconds
@21:22:02.67 ARV.dial :air1
@21:22:03.53 adding ARV air
@21:22:03.54 delay 2 seconds
@21:22:05.61 AS.seek 0.536ml
@21:22:11.97 delay 5 seconds
@21:22:16.99 ARV.dial :carrier
@21:22:17.39 delay 7 seconds
@21:22:24.53 AS.seek 0.594ml
@21:23:23.03 delay 5 seconds
@21:23:28.05 ATV.dial :ASV
@21:23:28.46 delay 2 seconds
@21:23:30.53 AS.seek 0.6ml
@21:23:36.88 delay 4 seconds
@21:23:40.90 ATV.dial ARV
@21:23:41.46 delay 2 seconds
@21:23:43.48 ARV.dial :carrier
@21:23:43.49 delay 2 seconds
@21:23:45.56 AS.seek 0.65ml
@21:24:36.06 delay 4 seconds
@21:24:40.08 ATV.dial PCR
@21:24:40.71 ASV.dial :waste1
@21:24:40.72 ARV.dial :air1
@21:24:41.12 Positioning Slug by 0.324ml
@21:24:41.19 AS.seek 0.326ml
@21:30:07.16 delay 5 seconds
@21:30:12.18 ATV.dial ARV
@21:30:13.08 ASV.dial :PCRlock
@21:30:13.47 delay 5 seconds
@21:30:18.49 Running AIPc ProfileIPc thermocycling
@21:30:18.50 <PCR> PCR.program ProfileIPc
@21:30:18.60 ProfileIPc should complete in < 1:26:35
@21:30:18.61 PCR.run 1:53:13.75
@22:39:04.13 PCR.off
@22:39:04.68 <MFBPCR> PCR for AIPc completed -- closed /var/log/esp/AIPc21apr2621h.pcr
@22:39:04.69 Full MFB Decontamination
@22:39:04.70 Decon extraction column and decon extSrc
@22:39:04.72 SPE.hold 2:46:40,55C
@22:39:04.76 ASV.dial :SPEdelivery
@22:39:05.42 delay 20 seconds
@22:39:25.45 AFV.select :carrierSolution
@22:39:25.55 delay 2 seconds
@22:39:27.58 AS.reconfigure ASconfig
@22:39:27.62 AS.seek 0.5ml
@22:39:37.94 delay 1 second
@22:39:38.96 AFV.select :holdingCoil
@22:39:39.06 ATV.dial :air1
@22:39:41.11 AS.reconfigure ASslow1
@22:39:41.16 AS.seek 0.52ml
@22:39:45.47 ATV.dial :ASV
@22:39:47.41 ASV.dial :SPErecovery
@22:39:48.09 AS.seek 0.62ml
@22:40:08.58 ATV.dial :waste
@22:40:10.78 AS.reconfigure ASnorm
@22:40:10.83 AS.seek 0ml
@22:40:42.39 deconEluateRecoveryLine extSrc
@22:40:42.40 ATV.dial :waste
@22:40:42.42 AS.seek 0ml
@22:40:42.47 ATV.dial :air3
@22:40:43.23 AS.reconfigure ASslow1
@22:40:43.27 AS.seek 0.01ml
@22:40:45.56 delay 2 seconds
@22:40:47.58 ATV.dial :bleach
@22:40:48.08 AS.seek 0.31ml
@22:41:48.66 delay 3 seconds
@22:41:51.68 ATV.dial :air3
@22:41:52.28 delay 3 seconds
@22:41:55.35 AS.seek 0.335ml
@22:42:00.55 delay 3 seconds
@22:42:03.57 ATV.dial :bleach
@22:42:04.23 AS.seek 0.32ml
@22:42:07.52 delay 3 seconds
@22:42:10.54 ATV.dial :extSrc
@22:42:11.20 AS.seek 0ml
@22:43:15.83 AFV.select :carrierSolution
@22:43:15.93 delay 2 seconds
@22:43:17.96 AS.reconfigure ASconfig
@22:43:18.00 AS.seek 0.5ml
@22:43:47.02 delay 1 second
@22:43:48.04 AFV.select :holdingCoil
@22:43:48.14 ATV.dial :extSrc
@22:43:48.16 AS.reconfigure ASslow1
@22:43:48.20 AS.seek 0ml
@22:45:29.03 AFV.select :carrierSolution
@22:45:29.13 delay 2 seconds
@22:45:31.16 AS.reconfigure ASconfig
@22:45:31.20 AS.seek 0.5ml
@22:46:00.24 delay 1 second
@22:46:01.26 AFV.select :holdingCoil
@22:46:01.36 ATV.dial :extSrc
@22:46:01.38 AS.reconfigure ASslow1
@22:46:01.42 AS.seek 0ml
@22:47:42.27 AFV.select :carrierSolution
@22:47:42.37 delay 2 seconds
@22:47:44.40 AS.reconfigure ASconfig
@22:47:44.44 AS.seek 0.5ml
@22:48:13.45 delay 1 second
@22:48:14.47 AFV.select :holdingCoil
@22:48:14.57 ATV.dial :extSrc
@22:48:14.59 AS.reconfigure ASslow1
@22:48:14.63 AS.seek 0ml
@22:49:55.59 AFV.select :carrierSolution
@22:49:55.69 delay 2 seconds
@22:49:57.72 AS.reconfigure ASconfig
@22:49:57.76 AS.seek 0.5ml
@22:50:26.77 delay 1 second
@22:50:27.79 AFV.select :holdingCoil
@22:50:27.89 ATV.dial :extSrc
@22:50:27.91 AS.reconfigure ASslow1
@22:50:27.95 AS.seek 0ml
@22:52:09.20 ATV.dial :air2
@22:52:10.58 AS.reconfigure ASslow0
@22:52:10.62 AS.seek 0.5ml
@22:53:01.25 ATV.dial :extSrc
@22:53:02.80 AS.seek 0.02ml
@22:53:51.42 delay 1 second
@22:53:52.44 ATV.dial :waste
@22:53:53.80 AS.reconfigure ASconfig
@22:53:53.85 AS.seek 0ml
@22:53:55.28 AFV.select :holdingCoil
@22:53:55.29 ATV.dial :waste
@22:53:55.31 AS.seek 0ml
@22:53:55.36 AFV.select :carrierSolution
@22:53:55.46 delay 2 seconds
@22:53:57.49 AS.seek 0.5ml
@22:54:26.49 delay 1 second
@22:54:27.51 AFV.select :holdingCoil
@22:54:27.61 ATV.dial :air3
@22:54:28.50 AS.reconfigure ASslow1
@22:54:28.54 AS.seek 0.52ml
@22:54:32.74 ATV.dial :air3
@22:54:32.81 AS.seek 0.53ml
@22:54:35.24 delay 2 seconds
@22:54:37.26 ATV.dial :bleach
@22:54:37.72 AS.seek 0.71ml
@22:55:14.19 delay 3 seconds
@22:55:17.21 ATV.dial :air3
@22:55:17.78 delay 3 seconds
@22:55:20.85 AS.seek 0.735ml
@22:55:26.29 delay 3 seconds
@22:55:29.31 ATV.dial :bleach
@22:55:29.95 AS.seek 0.72ml
@22:55:33.33 delay 3 seconds
@22:55:36.35 ATV.dial ASV
@22:55:37.44 ASV.dial :SPErecovery
@22:55:37.51 AS.seek 0.365ml
@22:56:49.28 ATV.dial :waste
@22:56:51.28 AS.reconfigure ASnorm
@22:56:51.33 AS.seek 0ml
@22:57:10.03 ATV.dial :air3
@22:57:10.89 AS.reconfigure ASslow1
@22:57:10.94 AS.seek 0.01ml
@22:57:13.27 delay 2 seconds
@22:57:15.29 ATV.dial :bleach
@22:57:15.78 AS.seek 0.58ml
@22:59:10.67 delay 3 seconds
@22:59:13.69 ATV.dial :air3
@22:59:14.27 delay 3 seconds
@22:59:17.34 AS.seek 0.605ml
@22:59:22.63 delay 3 seconds
@22:59:25.65 ATV.dial :bleach
@22:59:26.28 AS.seek 0.59ml
@22:59:29.66 delay 3 seconds
@22:59:32.68 ATV.dial :ASV
@22:59:33.77 ASV.dial :wasteR
@22:59:34.16 AS.seek 0ml
@23:01:33.20 delay 1:00
@23:02:33.23 ATV.dial ASV
@23:02:33.24 AFV.select :carrierSolution
@23:02:33.34 delay 2 seconds
@23:02:35.36 AS.reconfigure ASconfig
@23:02:35.41 AS.seek 1ml
@23:03:33.14 delay 1 second
@23:03:34.16 AFV.select :holdingCoil
@23:03:34.26 delay 1 second
@23:03:35.28 ASV.dial :wasteR
@23:03:35.30 AS.reconfigure ASslow1
@23:03:35.34 AS.seek 0ml
@23:06:56.74 delay 10 seconds
@23:07:06.77 ATV.dial :air3
@23:07:08.36 AS.seek 0.01ml
@23:07:10.66 delay 2 seconds
@23:07:12.68 ATV.dial :bleach
@23:07:13.31 AS.seek 0.58ml
@23:09:08.25 delay 3 seconds
@23:09:11.27 ATV.dial :air3
@23:09:11.84 delay 3 seconds
@23:09:14.91 AS.seek 0.605ml
@23:09:20.31 delay 3 seconds
@23:09:23.33 ATV.dial :bleach
@23:09:23.97 AS.seek 0.59ml
@23:09:27.34 delay 3 seconds
@23:09:30.36 ATV.dial ASV
@23:09:31.45 ASV.dial :SPEdelivery
@23:09:31.83 AS.seek 0ml
@23:11:30.88 delay 1:00
@23:12:30.91 AFV.select :carrierSolution
@23:12:31.01 delay 2 seconds
@23:12:33.04 AS.reconfigure ASconfig
@23:12:33.08 AS.seek 1ml
@23:13:30.81 delay 1 second
@23:13:31.83 AFV.select :holdingCoil
@23:13:31.93 delay 1 second
@23:13:32.95 ATV.dial ASV
@23:13:32.96 ASV.dial :SPEdelivery
@23:13:32.98 AS.reconfigure ASslow1
@23:13:33.02 AS.seek 0ml
@23:16:54.39 delay 10 seconds
@23:17:04.42 ATV.dial :air2
@23:17:05.30 AS.reconfigure ASslow0
@23:17:05.35 AS.seek 0.8ml
@23:18:26.16 delay 3 seconds
@23:18:29.18 ATV.dial :ASV
@23:18:30.22 ASV.dial :SPEdelivery
@23:18:30.24 AS.reconfigure ASslow1
@23:18:30.28 AS.seek 0.03ml
@23:21:05.38 delay 20 seconds
@23:21:25.41 ATV.dial :waste,via: :air3
@23:21:27.68 AS.reconfigure ASconfig
@23:21:27.72 AS.seek 0ml
@23:21:29.75 SPE.coast
@23:21:29.82 Removing Bleach from Mixing Coil
@23:21:29.83 AFV.select :carrierSolution
@23:21:29.92 delay 2 seconds
@23:21:31.95 AS.seek 0.5ml
@23:22:00.95 delay 1 second
@23:22:01.97 AFV.select :holdingCoil
@23:22:02.07 ATV.dial :ASV
@23:22:04.02 ASV.dial :SPErecovery
@23:22:04.67 AS.reconfigure ASslow1
@23:22:04.71 AS.seek 0.73ml
@23:22:51.24 ASV.dial :wasteD
@23:22:51.83 AS.reconfigure ASnorm
@23:22:51.88 AS.seek 0ml
@23:23:28.97 AFV.select :carrierSolution
@23:23:29.07 delay 2 seconds
@23:23:31.10 AS.reconfigure ASconfig
@23:23:31.14 AS.seek 0.5ml
@23:24:00.16 delay 1 second
@23:24:01.18 AFV.select :holdingCoil
@23:24:01.28 ATV.dial ASV
@23:24:01.30 ASV.dial :SPErecovery
@23:24:01.96 AS.reconfigure ASslow0
@23:24:02.01 AS.seek 0.3ml
@23:24:22.47 ATV.dial :air2
@23:24:23.38 AS.reconfigure ASslow1
@23:24:23.43 AS.seek 0.32ml
@23:24:27.82 ATV.dial ASV
@23:24:28.90 AS.reconfigure ASslow0
@23:24:28.94 AS.seek 0.57ml
@23:24:54.41 ASV.dial :wasteD
@23:24:55.02 AS.reconfigure ASnorm
@23:24:55.07 AS.seek 0ml
@23:25:24.09 AFV.select :carrierSolution
@23:25:24.19 delay 2 seconds
@23:25:26.22 AS.reconfigure ASconfig
@23:25:26.26 AS.seek 0.5ml
@23:25:55.30 delay 1 second
@23:25:56.32 AFV.select :holdingCoil
@23:25:56.42 ATV.dial ASV
@23:25:56.44 ASV.dial :SPErecovery
@23:25:57.10 AS.reconfigure ASslow0
@23:25:57.15 AS.seek 0.3ml
@23:26:17.55 ATV.dial :air2
@23:26:18.61 AS.reconfigure ASslow1
@23:26:18.66 AS.seek 0.32ml
@23:26:23.02 ATV.dial ASV
@23:26:24.11 AS.reconfigure ASslow0
@23:26:24.16 AS.seek 0.57ml
@23:26:49.66 ASV.dial :wasteD
@23:26:50.27 AS.reconfigure ASnorm
@23:26:50.32 AS.seek 0ml
@23:27:19.34 ATV.dial :air2
@23:27:20.34 AS.reconfigure ASslow0
@23:27:20.39 AS.seek 0.3ml
@23:27:50.84 ATV.dial :ASV
@23:27:51.88 ASV.dial :wasteD
@23:27:51.89 AS.reconfigure ASconfig
@23:27:51.94 AS.seek 0.05ml
@23:28:06.61 ATV.dial :waste
@23:28:08.58 AS.seek 0ml
@23:28:11.73 ASV.dial :SPEdelivery
@23:28:12.80 Cleaning PCR
@23:28:12.81 AFV.select :carrierSolution
@23:28:12.91 delay 2 seconds
@23:28:14.94 AS.seek 0.5ml
@23:28:43.95 delay 1 second
@23:28:44.97 AFV.select :holdingCoil
@23:28:45.07 ASV.dial :waste1
@23:28:45.44 ATV.dial :air3
@23:28:46.13 AS.reconfigure ASslow1
@23:28:46.18 AS.seek 0.51ml
@23:28:48.45 delay 2 seconds
@23:28:50.47 ATV.dial :bleach
@23:28:50.93 AS.seek 0.81ml
@23:29:51.52 delay 3 seconds
@23:29:54.54 ATV.dial :air3
@23:29:55.11 delay 3 seconds
@23:29:58.18 AS.seek 0.835ml
@23:30:03.45 delay 3 seconds
@23:30:06.47 ATV.dial :bleach
@23:30:07.10 AS.seek 0.82ml
@23:30:10.47 delay 3 seconds
@23:30:13.49 ARV.dial :waste
@23:30:14.11 delay 3 seconds
@23:30:17.13 ATV.dial :ARV
@23:30:18.04 AS.reconfigure ASconfig
@23:30:18.09 AS.seek 0.47ml
@23:30:38.49 AFV.select :carrierSolution
@23:30:38.59 delay 2 seconds
@23:30:40.62 AS.seek 1ml
@23:31:11.38 delay 1 second
@23:31:12.40 AFV.select :holdingCoil
@23:31:12.50 delay 1 second
@23:31:13.53 AS.reconfigure ASfast
@23:31:13.57 AS.seek 0ml
@23:31:40.91 AFV.select :carrierSolution
@23:31:41.01 delay 2 seconds
@23:31:43.03 AS.reconfigure ASconfig
@23:31:43.08 AS.seek 1ml
@23:32:40.88 delay 1 second
@23:32:41.90 AFV.select :holdingCoil
@23:32:42.00 delay 1 second
@23:32:43.03 AS.reconfigure ASfast
@23:32:43.07 AS.seek 0ml
@23:33:10.41 AFV.select :carrierSolution
@23:33:10.51 delay 2 seconds
@23:33:12.53 AS.reconfigure ASconfig
@23:33:13.01 AS.seek 1ml
@23:34:10.80 delay 1 second
@23:34:11.82 AFV.select :holdingCoil
@23:34:11.92 delay 1 second
@23:34:12.95 AS.reconfigure ASfast
@23:34:12.99 AS.seek 0ml
@23:34:40.33 ARV.dial between carrier and air1
@23:34:40.84 ATV.dial :air3
@23:34:42.19 AS.reconfigure ASslow1
@23:34:42.23 AS.seek 0.01ml
@23:34:44.61 delay 2 seconds
@23:34:46.63 ATV.dial :bleach
@23:34:47.26 AS.seek 0.81ml
@23:37:28.38 delay 3 seconds
@23:37:31.40 ATV.dial :air3
@23:37:31.97 delay 3 seconds
@23:37:35.04 AS.seek 0.835ml
@23:37:40.39 delay 3 seconds
@23:37:43.41 ATV.dial :bleach
@23:37:44.05 AS.seek 0.82ml
@23:37:47.33 delay 3 seconds
@23:37:50.35 ATV.dial PCR
@23:37:50.77 AS.reconfigure ASconfig
@23:37:50.82 AS.seek 0ml
@23:38:38.24 PCR.program :bleachProfile
@23:38:38.30 PCR program should complete in < 2:45
@23:38:38.31 PCR.run 10:00
@23:41:40.14 PCR.off
@23:41:40.17 AFV.select :carrierSolution
@23:41:40.27 delay 2 seconds
@23:41:42.30 AS.seek 1ml
@23:42:40.08 delay 1 second
@23:42:41.10 AFV.select :holdingCoil
@23:42:41.20 delay 1 second
@23:42:42.23 AS.reconfigure ASfast
@23:42:42.27 AS.seek 0ml
@23:43:09.61 delay 3 seconds
@23:43:12.63 AFV.select :carrierSolution
@23:43:12.73 delay 2 seconds
@23:43:14.75 AS.reconfigure ASconfig
@23:43:14.81 AS.seek 1ml
@23:44:12.53 delay 1 second
@23:44:13.55 AFV.select :holdingCoil
@23:44:13.65 delay 1 second
@23:44:14.68 AS.reconfigure ASfast
@23:44:14.72 AS.seek 0ml
@23:44:42.05 delay 3 seconds
@23:44:45.07 AFV.select :carrierSolution
@23:44:45.17 delay 2 seconds
@23:44:47.19 AS.reconfigure ASconfig
@23:44:47.28 AS.seek 1ml
@23:45:45.02 delay 1 second
@23:45:46.04 AFV.select :holdingCoil
@23:45:46.14 delay 1 second
@23:45:47.17 AS.reconfigure ASfast
@23:45:47.21 AS.seek 0ml
@23:46:14.55 delay 3 seconds
@23:46:17.57 AFV.select :carrierSolution
@23:46:17.67 delay 2 seconds
@23:46:19.69 AS.reconfigure ASconfig
@23:46:19.75 AS.seek 1ml
@23:47:17.89 delay 1 second
@23:47:18.91 AFV.select :holdingCoil
@23:47:19.01 delay 1 second
@23:47:20.04 AS.reconfigure ASfast
@23:47:20.08 AS.seek 0ml
@23:47:47.42 delay 3 seconds
@23:47:50.44 AFV.select :carrierSolution
@23:47:50.54 delay 2 seconds
@23:47:52.57 AS.reconfigure ASconfig
@23:47:52.61 AS.seek 1ml
@23:48:50.34 delay 1 second
@23:48:51.36 AFV.select :holdingCoil
@23:48:51.46 delay 1 second
@23:48:52.49 AS.reconfigure ASnorm
@23:48:52.53 AS.seek 0ml
@23:49:43.22 AFV.select :carrierSolution
@23:49:43.32 delay 2 seconds
@23:49:45.35 AS.reconfigure ASconfig
@23:49:45.39 AS.seek 1ml
@23:50:43.17 delay 1 second
@23:50:44.19 AFV.select :holdingCoil
@23:50:44.29 delay 1 second
@23:50:45.31 ATV.dial :waste
@23:50:46.59 AS.reconfigure ASnorm
@23:50:46.64 AS.seek 0.5ml
@23:51:12.19 ATV.dial :waste2
@23:51:13.53 AS.seek 0ml
@10:25:18.82CEST27-Apr-21 <MAIN> ESP2 v3.30 I2C Gateway v3.06 for core bus
@10:25:21.19 
Configuration for mer 7/20/20 brent@mbari.org
/home/esp/esp2/type/shallow/mer/configure.rb
@10:25:21.28 
Configuration for MFB3 by preston@mbari.org
/home/esp/esp2/type/mfb/MFB3/configure.rb
@10:25:26.59 Polling Can every 10:00
@10:25:28.36 CS.configure :default
@10:25:28.43 CS.hold
@10:25:28.55 CS.configure :default
@10:25:28.73 PS.configure :default
@10:25:28.89 SS.configure :default
@10:25:28.97 SS.hold
@10:25:29.07 SS.configure :default
@10:25:29.17 CC.configure :default
@10:25:29.21 CC.hold
@10:25:29.28 CC.configure :default
@10:25:29.36 PC.configure :default
@10:25:29.40 PC.hold
@10:25:29.45 PC.configure :default
@10:25:29.55 Forearm.configure :loose
@10:25:29.59 Forearm.hold
@10:25:29.65 Forearm.configure :loose
@10:25:29.73 Elbow.configure :loose
@10:25:29.76 Elbow.hold
@10:25:29.81 Elbow.configure :loose
@10:25:29.91 SC.configure :loose
@10:25:29.95 SC.hold
@10:25:30.00 SC.configure :loose
@10:25:30.08 SE.configure :default
@10:25:30.12 SE.hold
@10:25:30.18 SE.configure :default
@10:25:30.21 CH.coast
@10:25:30.26 CH.configure :default
@10:25:30.29 PH.coast
@10:25:30.34 PH.configure :default
@10:25:30.44 SPE.coast
@10:25:30.49 SPE.configure SPEconfig
@10:25:30.57 AS.configure ASconfig
@10:25:30.61 AS.hold
@10:25:30.65 AS.configure ASconfig
@10:25:30.69 PCR.off
@10:25:30.72 PCR.pidtemp= 50,0.1,0,300,25,25
@10:25:30.76 PCR.caltemp= 2.5051,0.0372,0
@10:25:30.79 All dwarf controllers running firmware version 3.75
@10:25:31.02 Cleanly exited at 23:51:39CEST26-Apr-21
@10:25:31.05 Loading /home/esp/esp2/mission/GENOMAP_v2.rb mission
@10:25:31.11 Cleaning PCR
@10:25:31.12 AFV.select :carrierSolution
@10:25:31.21 delay 2 seconds
@10:25:32.78 <mwj> Session Begun
@10:25:33.24 <MAIN> AS.seek 0.5ml
@10:25:36.89 <mwj> -> exit
@10:25:36.91 Session Ended
@10:26:02.30 <MAIN> delay 1 second
@10:26:03.32 AFV.select :holdingCoil
@10:26:03.42 ASV.dial :waste1
@10:26:03.50 ATV.dial :air3
@10:26:05.55 AS.reconfigure ASslow1
@10:26:05.60 AS.seek 0.51ml
@10:26:07.87 delay 2 seconds
@10:26:09.89 ATV.dial :bleach
@10:26:10.37 AS.seek 0.81ml
@10:27:10.95 delay 3 seconds
@10:27:13.97 ATV.dial :air3
@10:27:14.55 delay 3 seconds
@10:27:17.62 AS.seek 0.835ml
@10:27:22.90 delay 3 seconds
@10:27:25.92 ATV.dial :bleach
@10:27:26.56 AS.seek 0.82ml
@10:27:29.84 delay 3 seconds
@10:27:32.86 ARV.dial :waste
@10:27:33.37 delay 3 seconds
@10:27:36.39 ATV.dial :ARV
@10:27:37.30 AS.reconfigure ASconfig
@10:27:37.35 AS.seek 0.47ml
@10:27:57.73 AFV.select :carrierSolution
@10:27:57.83 delay 2 seconds
@10:27:59.86 AS.seek 1ml
@10:28:30.59 delay 1 second
@10:28:31.61 AFV.select :holdingCoil
@10:28:31.71 delay 1 second
@10:28:32.74 AS.reconfigure ASfast
@10:28:32.78 AS.seek 0ml
@10:29:00.11 AFV.select :carrierSolution
@10:29:00.21 delay 2 seconds
@10:29:02.23 AS.reconfigure ASconfig
@10:29:02.28 AS.seek 1ml
@10:30:00.00 delay 1 second
@10:30:01.02 AFV.select :holdingCoil
@10:30:01.12 delay 1 second
@10:30:02.15 AS.reconfigure ASfast
@10:30:02.19 AS.seek 0ml
@10:30:29.51 AFV.select :carrierSolution
@10:30:29.61 delay 2 seconds
@10:30:31.63 AS.reconfigure ASconfig
@10:30:31.68 AS.seek 1ml
@10:31:29.78 delay 1 second
@10:31:30.80 AFV.select :holdingCoil
@10:31:30.90 delay 1 second
@10:31:31.93 AS.reconfigure ASfast
@10:31:31.97 AS.seek 0ml
@10:31:59.31 ARV.dial between carrier and air1
@10:31:59.83 ATV.dial :air3
@10:32:01.17 AS.reconfigure ASslow1
@10:32:01.21 AS.seek 0.01ml
@10:32:03.59 delay 2 seconds
@10:32:05.61 ATV.dial :bleach
@10:32:06.25 AS.seek 0.81ml
@10:34:47.51 delay 3 seconds
@10:34:50.53 ATV.dial :air3
@10:34:51.11 delay 3 seconds
@10:34:54.18 AS.seek 0.835ml
@10:34:59.46 delay 3 seconds
@10:35:02.48 ATV.dial :bleach
@10:35:03.12 AS.seek 0.82ml
@10:35:06.51 delay 3 seconds
@10:35:09.53 ATV.dial PCR
@10:35:09.95 AS.reconfigure ASconfig
@10:35:09.99 AS.seek 0ml
@10:35:57.42 PCR.program :bleachProfile
@10:35:57.47 PCR program should complete in < 2:45
@10:35:57.48 PCR.run 10:00
@10:38:59.32 PCR.off
@10:38:59.35 AFV.select :carrierSolution
@10:38:59.45 delay 2 seconds
@10:39:01.48 AS.seek 1ml
@10:39:59.20 delay 1 second
@10:40:00.22 AFV.select :holdingCoil
@10:40:00.32 delay 1 second
@10:40:01.35 AS.reconfigure ASfast
@10:40:01.39 AS.seek 0ml
@10:40:28.72 delay 3 seconds
@10:40:31.74 AFV.select :carrierSolution
@10:40:31.84 delay 2 seconds
@10:40:33.86 AS.reconfigure ASconfig
@10:40:33.91 AS.seek 1ml
@10:41:31.64 delay 1 second
@10:41:32.66 AFV.select :holdingCoil
@10:41:32.76 delay 1 second
@10:41:33.79 AS.reconfigure ASfast
@10:41:33.83 AS.seek 0ml
@10:42:01.16 delay 3 seconds
@10:42:04.18 AFV.select :carrierSolution
@10:42:04.28 delay 2 seconds
@10:42:06.30 AS.reconfigure ASconfig
@10:42:06.35 AS.seek 1ml
@10:43:04.08 delay 1 second
@10:43:05.10 AFV.select :holdingCoil
@10:43:05.20 delay 1 second
@10:43:06.23 AS.reconfigure ASfast
@10:43:06.27 AS.seek 0ml
@10:43:33.58 delay 3 seconds
@10:43:36.60 AFV.select :carrierSolution
@10:43:36.70 delay 2 seconds
@10:43:38.72 AS.reconfigure ASconfig
@10:43:38.77 AS.seek 1ml
@10:44:36.55 delay 1 second
@10:44:37.57 AFV.select :holdingCoil
@10:44:37.67 delay 1 second
@10:44:38.70 AS.reconfigure ASfast
@10:44:38.74 AS.seek 0ml
@10:45:06.06 delay 3 seconds
@10:45:09.08 AFV.select :carrierSolution
@10:45:09.18 delay 2 seconds
@10:45:11.20 AS.reconfigure ASconfig
@10:45:11.26 AS.seek 1ml
@10:46:09.00 delay 1 second
@10:46:10.01 AFV.select :holdingCoil
@10:46:10.10 delay 1 second
@10:46:11.13 AS.reconfigure ASnorm
@10:46:11.17 AS.seek 0ml
@11:15:29.01CEST27-Apr-21 <MAIN> ESP2 v3.30 I2C Gateway v3.06 for core bus
@11:15:31.09 
Configuration for mer 7/20/20 brent@mbari.org
/home/esp/esp2/type/shallow/mer/configure.rb
@11:15:31.17 
Configuration for MFB3 by preston@mbari.org
/home/esp/esp2/type/mfb/MFB3/configure.rb
@11:15:35.49 Polling Can every 10:00
@11:15:37.20 CS.configure :default
@11:15:37.24 CS.hold
@11:15:37.31 CS.configure :default
@11:15:37.39 PS.configure :default
@11:15:37.47 SS.configure :default
@11:15:37.50 SS.hold
@11:15:37.56 SS.configure :default
@11:15:37.64 CC.configure :default
@11:15:37.67 CC.hold
@11:15:37.75 CC.configure :default
@11:15:37.83 PC.configure :default
@11:15:37.86 PC.hold
@11:15:37.94 PC.configure :default
@11:15:38.03 Forearm.configure :loose
@11:15:38.07 Forearm.hold
@11:15:38.13 Forearm.configure :loose
@11:15:38.20 Elbow.configure :loose
@11:15:38.24 Elbow.hold
@11:15:38.30 Elbow.configure :loose
@11:15:38.39 SC.configure :loose
@11:15:38.43 SC.hold
@11:15:38.48 SC.configure :loose
@11:15:38.56 SE.configure :default
@11:15:38.59 SE.hold
@11:15:38.63 SE.configure :default
@11:15:38.67 CH.coast
@11:15:38.72 CH.configure :default
@11:15:38.74 PH.coast
@11:15:38.79 PH.configure :default
@11:15:38.88 SPE.coast
@11:15:38.93 SPE.configure SPEconfig
@11:15:39.00 AS.configure ASconfig
@11:15:39.04 AS.hold
@11:15:39.08 AS.configure ASconfig
@11:15:39.13 PCR.off
@11:15:39.16 PCR.pidtemp= 50,0.1,0,300,25,25
@11:15:39.19 PCR.caltemp= 2.5051,0.0372,0
@11:15:39.22 All dwarf controllers running firmware version 3.75
@11:15:39.45 Cleanly exited at 11:14:59CEST27-Apr-21
@11:15:39.48 Loading /home/esp/esp2/mission/GENOMAP_v3.rb mission
@11:15:39.63 <MFBPCR> Extracting Nucleic Acid from extSrc
@11:15:39.65 SPE.hold 2:46:40,55C
@11:15:39.69 ASV.dial :SPEdelivery
@11:15:40.12 delay 20 seconds
@11:15:48.39 <bspe> Session Begun
@11:16:00.14 <MFBPCR> AFV.select :holdingCoil
@11:16:00.25 ATV.dial :air2
@11:16:01.51 AS.reconfigure ASslow1
@11:16:01.57 AS.seek 0.07ml
@11:16:15.95 delay 3 seconds
@11:16:18.97 ATV.dial :extSrc
@11:16:20.45 AS.seek 0.47ml
@11:17:41.34 delay 5 seconds
@11:17:46.41 Solid Phase Extraction Heater at 39C
@11:17:46.42 ATV.dial ASV
@11:17:47.30 ASV.dial :SPEdelivery
@11:17:47.38 AS.seek 0.042ml
@11:19:13.72 delay 10 seconds
@11:19:23.75 Rinsing external lysate line extSrc
@11:19:23.76 AFV.select :carrierSolution
@11:19:23.88 delay 2 seconds
@11:19:25.91 AS.reconfigure ASconfig
@11:19:25.96 AS.seek 1ml
@11:20:21.33 delay 1 second
@11:20:22.35 AFV.select :holdingCoil
@11:20:22.45 delay 1 second
@11:20:23.47 ATV.dial :extSrc
@11:20:24.31 AS.reconfigure ASnorm
@11:20:24.37 AS.seek 0ml
@11:21:15.03 ATV.dial :air2
@11:21:16.62 AS.reconfigure ASslow0
@11:21:16.68 AS.seek 0.05ml
@11:21:22.11 ATV.dial :extSrc
@11:21:23.70 AS.seek 0.01ml
@11:21:28.08 ATV.dial :waste
@11:21:29.50 AS.reconfigure ASconfig
@11:21:29.56 AS.seek 0ml
@11:21:30.44 ethanol wash
@11:21:30.45 ATV.dial :air2
@11:21:31.65 AS.reconfigure ASslow2
@11:21:31.71 AS.seek 0.01ml
@11:21:42.22 delay 3 seconds
@11:21:45.24 Pulling 0.4ml of Ethanol Wash
@11:21:45.25 ATV.dial :ethanol
@11:21:46.15 AS.reconfigure ASslow1
@11:21:46.20 AS.seek 0.41ml
@11:23:06.95 delay 3 seconds
@11:23:09.97 ATV.dial :air1
@11:23:10.41 delay 3 seconds
@11:23:13.48 AS.seek 0.435ml
@11:23:18.84 delay 3 seconds
@11:23:21.86 ATV.dial :ethanol
@11:23:22.53 AS.seek 0.42ml
@11:23:25.84 delay 3 seconds
@11:23:28.86 ATV.dial ASV
@11:23:30.26 AS.seek 0.093ml
@11:24:36.28 delay 3 seconds
@11:24:39.30 ATV.dial :waste2
@11:24:40.11 AS.reconfigure ASconfig
@11:24:40.17 AS.seek 0ml
@11:24:45.81 ATV.dial :air2
@11:24:46.22 AS.reconfigure ASslow1
@11:24:46.28 AS.seek 1ml
@11:28:07.78 delay 5 seconds
@11:28:12.80 ATV.dial ASV
@11:28:13.84 ASV.dial :SPEdelivery
@11:28:13.86 AS.seek 0.02ml
@11:31:31.22 delay 5 seconds
@11:31:36.24 ATV.dial :air2
@11:31:37.34 AS.seek 0.52ml
@11:33:18.19 delay 5 seconds
@11:33:23.21 ATV.dial ASV
@11:33:24.25 ASV.dial :wasteR
@11:33:24.64 AS.seek 0.3ml
@11:34:09.12 delay 20 seconds
@11:34:29.16 AS.seek 0.07ml
@11:35:15.83 delay 20 seconds
@11:35:35.85 ATV.dial :waste2
@11:35:36.66 AS.reconfigure ASconfig
@11:35:36.72 AS.seek 0ml
@11:35:41.03 Elution
@11:35:41.04 ATV.dial :air1
@11:35:42.25 AS.reconfigure ASslow1
@11:35:42.31 AS.seek 0.05ml
@11:35:52.81 delay 3 seconds
@11:35:55.83 ATV.dial :ElutionWater
@11:35:56.56 AS.seek 0.11ml
@11:36:08.92 delay 3 seconds
@11:36:11.94 ATV.dial ASV,via: :air2
@11:36:14.20 ASV.dial :SPEelution
@11:36:14.81 AFV.select :carrierSolution
@11:36:14.97 AS.reconfigure ASnorm
@11:36:15.02 AS.seek 0.76ml
@11:36:48.06 delay 3 seconds
@11:36:51.08 AFV.select :holdingCoil
@11:36:51.23 AS.reconfigure ASslow1
@11:36:51.28 AS.seek 0.475ml
@11:37:48.89 delay 5 seconds
@11:37:53.97 AS.reconfigure ASslow2
@11:37:54.03 AS.seek 0.44ml
@11:38:29.37 delay 1:00
@11:39:29.40 pushing eluate to holding coil
@11:39:29.47 AS.seek 0.28ml
@11:42:10.75 delay 30 seconds
@11:42:40.78 ATV.dial :waste,via: :air3
@11:42:42.62 AS.reconfigure ASconfig
@11:42:42.68 AS.seek 0ml
@11:42:59.09 Parking Bleach in LysateSource line and SPEcolumn
@11:42:59.10 ATV.dial :air3
@11:42:59.99 AS.reconfigure ASslow1
@11:43:00.05 AS.seek 0.03ml
@11:43:06.51 ATV.dial :ElutionWater
@11:43:07.15 AS.seek 0.06ml
@11:43:13.47 ATV.dial :air3
@11:43:14.11 AS.seek 0.07ml
@11:43:16.45 delay 2 seconds
@11:43:18.47 ATV.dial :bleach
@11:43:19.05 AS.seek 0.15ml
@11:43:35.47 delay 3 seconds
@11:43:38.49 ATV.dial :air3
@11:43:39.08 delay 3 seconds
@11:43:42.15 AS.seek 0.175ml
@11:43:47.61 delay 3 seconds
@11:43:50.63 ATV.dial :bleach
@11:43:51.28 AS.seek 0.16ml
@11:43:54.59 delay 3 seconds
@11:43:57.61 ATV.dial :extSrc
@11:43:58.27 AS.reconfigure ASconfig
@11:43:58.33 AS.seek 0.015ml
@11:44:06.97 ATV.dial :waste
@11:44:08.52 AS.seek 0ml
@11:44:09.67 ATV.dial :air3
@11:44:10.53 AS.reconfigure ASslow1
@11:44:10.59 AS.seek 0.01ml
@11:44:13.00 delay 2 seconds
@11:44:15.02 ATV.dial :bleach
@11:44:15.51 AS.seek 0.58ml
@11:46:10.42 delay 3 seconds
@11:46:13.44 ATV.dial :air3
@11:46:14.03 delay 3 seconds
@11:46:17.10 AS.seek 0.605ml
@11:46:22.48 delay 3 seconds
@11:46:25.50 ATV.dial :bleach
@11:46:26.14 AS.seek 0.59ml
@11:46:29.52 delay 3 seconds
@11:46:32.54 ATV.dial :ASV
@11:46:33.66 ASV.dial :wasteR
@11:46:34.28 AS.seek 0ml
@11:48:33.17 delay 1:00
@11:49:33.20 AFV.select :carrierSolution
@11:49:33.30 delay 2 seconds
@11:49:35.32 AS.reconfigure ASconfig
@11:49:35.38 AS.seek 1ml
@11:50:33.14 delay 1 second
@11:50:34.16 AFV.select :holdingCoil
@11:50:34.26 delay 1 second
@11:50:35.28 ATV.dial ASV
@11:50:35.29 ASV.dial :wasteR
@11:50:35.32 AS.reconfigure ASslow1
@11:50:35.38 AS.seek 0ml
@11:53:56.70 delay 20 seconds
@11:54:16.73 ATV.dial :air3
@11:54:18.34 AS.seek 0.59ml
@11:56:17.34 delay 3 seconds
@11:56:20.36 ATV.dial :ASV
@11:56:21.87 ASV.dial :wasteR
@11:56:21.95 AS.seek 0.09ml
@11:58:02.91 delay 30 seconds
@11:58:32.94 ATV.dial :extSrc
@11:58:33.78 delay 3 seconds
@11:58:36.80 ATV.dial :ElutionWater
@11:58:38.01 AS.seek 0.12ml
@11:58:44.42 ATV.dial :air3
@11:58:45.08 AS.seek 0.13ml
@11:58:47.39 delay 2 seconds
@11:58:49.41 ATV.dial :bleach
@11:58:49.92 AS.seek 0.23ml
@11:59:10.34 delay 3 seconds
@11:59:13.36 ATV.dial :air3
@11:59:13.95 delay 3 seconds
@11:59:17.02 AS.seek 0.255ml
@11:59:22.45 delay 3 seconds
@11:59:25.47 ATV.dial :bleach
@11:59:26.54 AS.seek 0.24ml
@11:59:29.81 delay 3 seconds
@11:59:32.83 ATV.dial :ASV
@11:59:33.95 ASV.dial :SPEdelivery
@11:59:34.34 AFV.select :carrierSolution
@11:59:34.45 delay 2 seconds
@11:59:36.48 AS.reconfigure ASconfig
@11:59:36.53 AS.seek 0.5ml
@11:59:51.78 delay 1 second
@11:59:52.80 AFV.select :holdingCoil
@11:59:52.95 AS.reconfigure ASslow1
@11:59:53.01 AS.seek 0.17ml
@12:00:59.65 SPE.hold 2:46:40,95C
@12:00:59.70 delay 3:00
@12:03:59.75 AFV.select :carrierSolution
@12:03:59.86 delay 2 seconds
@12:04:01.89 AS.reconfigure ASconfig
@12:04:01.94 AS.seek 1ml
@12:04:49.95 delay 1 second
@12:04:50.97 AFV.select :holdingCoil
@12:04:51.07 delay 1 second
@12:04:52.09 ASV.dial :wasteD
@12:04:53.22 AS.seek 0ml
@12:05:51.02 Priming Eluate
@12:05:51.03 AFV.select :carrierSolution
@12:05:51.15 delay 2 seconds
@12:05:53.18 AS.seek 0.5ml
@12:06:22.22 delay 1 second
@12:06:23.24 AFV.select :holdingCoil
@12:06:23.34 ATV.dial ASV
@12:06:23.35 ASV.dial :SPErecovery
@12:06:24.02 Swish #1 of 2
@12:06:24.10 AS.reconfigure ASslow0
@12:06:24.16 AS.seek 0.6ml
@12:06:34.63 AS.seek 0.5ml
@12:06:45.09 Swish #2 of 2
@12:06:45.17 AS.seek 0.6ml
@12:06:55.67 AS.seek 0.5ml
@12:07:06.03 delay 3 seconds
@12:07:09.10 AS.reconfigure ASslow1
@12:07:09.16 AS.seek 0.684ml
@12:07:46.45 delay 3 seconds
@12:07:49.47 Eluate primed
@12:07:49.48 ATV.dial :waste2
@12:07:50.17 AS.reconfigure ASnorm
@12:07:50.23 AS.seek 0ml
@12:08:25.03 SPE.coast
@12:08:25.11 ASV.dial :SPErecovery
@12:08:25.13 Setting up PCR for SYBRIPc -- writing /var/log/esp/SYBRIPc21apr2712h.pcr
@12:08:25.15 Building PCR Slug from ASV with MM1 mix, PP6 primer
@12:08:25.17 Priming with carrier
@12:08:25.19 ATV.dial ARV
@12:08:26.25 ARV.dial :carrier
@12:08:26.61 AS.seek 0.5ml
@12:08:52.19 AFV.select :carrierSolution
@12:08:52.31 delay 2 seconds
@12:08:54.34 AS.reconfigure ASconfig
@12:08:54.39 AS.seek 1ml
@12:09:23.41 delay 1 second
@12:09:24.43 AFV.select :holdingCoil
@12:09:24.53 delay 1 second
@12:09:25.55 ATV.dial :waste
@12:09:27.17 AS.reconfigure ASnorm
@12:09:27.23 AS.seek 0ml
@12:10:17.94 AFV.select :carrierSolution
@12:10:18.06 delay 2 seconds
@12:10:20.09 AS.reconfigure ASconfig
@12:10:20.14 AS.seek 0.5ml
@12:10:49.19 delay 1 second
@12:10:50.21 AFV.select :holdingCoil
@12:10:50.31 Building Slug
@12:10:50.33 ATV.dial :ARV
@12:10:52.09 delay 2 seconds
@12:10:54.11 ARV.dial :air1
@12:10:54.53 delay 2 seconds
@12:10:56.60 AS.reconfigure ASslow2
@12:10:56.66 AS.seek 0.506ml
@12:11:02.84 delay 2 seconds
@12:11:04.86 ARV.dial :MM1
@12:11:05.50 delay 2 seconds
@12:11:07.52 Adding MM1
@12:11:07.60 AS.seek 0.524ml
@12:11:25.86 delay 25 seconds
@12:11:50.88 ARV.dial :PP6
@12:11:52.23 Adding PP6
@12:11:52.24 delay 2 seconds
@12:11:54.32 AS.seek 0.53ml
@12:12:00.45 delay 10 seconds
@12:12:10.48 ARV.dial :air1
@12:12:11.36 adding ARV air
@12:12:11.37 delay 2 seconds
@12:12:13.45 AS.seek 0.536ml
@12:12:19.95 delay 5 seconds
@12:12:24.97 ARV.dial :carrier
@12:12:25.39 delay 7 seconds
@12:12:32.47 AS.seek 0.594ml
@12:13:31.16 delay 5 seconds
@12:13:36.18 ATV.dial :ASV
@12:13:36.61 delay 2 seconds
@12:13:38.68 AS.seek 0.6ml
@12:13:44.97 delay 4 seconds
@12:13:48.99 ATV.dial ARV
@12:13:49.58 delay 2 seconds
@12:13:51.60 ARV.dial :carrier
@12:13:51.61 delay 2 seconds
@12:13:53.69 AS.seek 0.65ml
@12:14:44.11 delay 4 seconds
@12:14:48.13 ATV.dial PCR
@12:14:48.77 ASV.dial :waste1
@12:14:49.58 ARV.dial :air1
@12:14:49.98 Positioning Slug by 0.324ml
@12:14:50.07 AS.seek 0.326ml
@12:20:16.52 delay 5 seconds
@12:20:21.54 ATV.dial ARV
@12:20:22.36 ASV.dial :PCRlock
@12:20:22.75 delay 5 seconds
@12:20:27.77 Running SYBRIPc Profile60CIQ1 thermocycling
@12:20:27.79 <PCR> PCR.program Profile60CIQ1
@12:20:27.91 Profile60CIQ1 should complete in < 1:56:15
@12:20:27.92 PCR.run 2:30:18.75
@13:58:57.19 PCR.off
@13:58:57.74 <MFBPCR> PCR for SYBRIPc completed -- closed /var/log/esp/SYBRIPc21apr2712h.pcr
@13:58:57.82 Cleaning PCR
@13:58:57.83 AFV.select :carrierSolution
@13:58:57.95 delay 2 seconds
@13:58:59.98 AS.reconfigure ASconfig
@13:59:00.03 AS.seek 0.5ml
@13:59:10.33 delay 1 second
@13:59:11.35 AFV.select :holdingCoil
@13:59:11.45 ASV.dial :waste1
@13:59:11.86 ATV.dial :air3
@13:59:13.23 AS.reconfigure ASslow1
@13:59:13.28 AS.seek 0.51ml
@13:59:15.58 delay 2 seconds
@13:59:17.60 ATV.dial :bleach
@13:59:18.26 AS.seek 0.81ml
@14:00:18.83 delay 3 seconds
@14:00:21.85 ATV.dial :air3
@14:00:22.44 delay 3 seconds
@14:00:25.51 AS.seek 0.835ml
@14:00:30.81 delay 3 seconds
@14:00:33.83 ATV.dial :bleach
@14:00:34.48 AS.seek 0.82ml
@14:00:37.77 delay 3 seconds
@14:00:40.79 ARV.dial :waste
@14:00:41.42 delay 3 seconds
@14:00:44.44 ATV.dial :ARV
@14:00:45.38 AS.reconfigure ASconfig
@14:00:45.44 AS.seek 0.47ml
@14:01:05.83 AFV.select :carrierSolution
@14:01:05.94 delay 2 seconds
@14:01:07.97 AS.seek 1ml
@14:01:38.72 delay 1 second
@14:01:39.74 AFV.select :holdingCoil
@14:01:39.84 delay 1 second
@14:01:40.87 AS.reconfigure ASfast
@14:01:40.92 AS.seek 0ml
@14:02:08.25 AFV.select :carrierSolution
@14:02:08.36 delay 2 seconds
@14:02:10.38 AS.reconfigure ASconfig
@14:02:10.44 AS.seek 1ml
@14:03:08.24 delay 1 second
@14:03:09.26 AFV.select :holdingCoil
@14:03:09.36 delay 1 second
@14:03:10.38 AS.reconfigure ASfast
@14:03:10.44 AS.seek 0ml
@14:03:37.78 AFV.select :carrierSolution
@14:03:37.89 delay 2 seconds
@14:03:39.91 AS.reconfigure ASconfig
@14:03:39.97 AS.seek 1ml
@14:04:37.72 delay 1 second
@14:04:38.74 AFV.select :holdingCoil
@14:04:38.84 delay 1 second
@14:04:39.86 AS.reconfigure ASfast
@14:04:39.92 AS.seek 0ml
@14:05:07.27 ARV.dial between carrier and air1
@14:05:07.80 ATV.dial :air3
@14:05:09.16 AS.reconfigure ASslow1
@14:05:09.22 AS.seek 0.01ml
@14:05:11.83 delay 2 seconds
@14:05:13.85 ATV.dial :bleach
@14:05:14.50 AS.seek 0.81ml
@14:07:55.89 delay 3 seconds
@14:07:58.91 ATV.dial :air3
@14:07:59.50 delay 3 seconds
@14:08:02.97 AS.seek 0.835ml
@14:08:08.28 delay 3 seconds
@14:08:11.30 ATV.dial :bleach
@14:08:11.95 AS.seek 0.82ml
@14:08:15.38 delay 3 seconds
@14:08:18.40 ATV.dial PCR
@14:08:18.83 AS.reconfigure ASconfig
@14:08:18.89 AS.seek 0ml
@14:09:06.31 PCR.program :bleachProfile
@14:09:06.37 PCR program should complete in < 2:45
@14:09:06.38 PCR.run 10:00
@14:12:05.20 PCR.off
@14:12:05.24 AFV.select :carrierSolution
@14:12:05.35 delay 2 seconds
@14:12:07.38 AS.seek 1ml
@14:13:05.17 delay 1 second
@14:13:06.19 AFV.select :holdingCoil
@14:13:06.29 delay 1 second
@14:13:07.32 AS.reconfigure ASfast
@14:13:07.37 AS.seek 0ml
@14:13:34.69 delay 3 seconds
@14:13:37.71 AFV.select :carrierSolution
@14:13:37.81 delay 2 seconds
@14:13:39.83 AS.reconfigure ASconfig
@14:13:39.90 AS.seek 1ml
@14:14:37.64 delay 1 second
@14:14:38.66 AFV.select :holdingCoil
@14:14:38.76 delay 1 second
@14:14:39.78 AS.reconfigure ASfast
@14:14:39.84 AS.seek 0ml
@14:15:07.17 delay 3 seconds
@14:15:10.19 AFV.select :carrierSolution
@14:15:10.29 delay 2 seconds
@14:15:12.31 AS.reconfigure ASconfig
@14:15:12.38 AS.seek 1ml
@14:16:10.13 delay 1 second
@14:16:11.15 AFV.select :holdingCoil
@14:16:11.25 delay 1 second
@14:16:12.28 AS.reconfigure ASfast
@14:16:12.33 AS.seek 0ml
@14:16:39.64 delay 3 seconds
@14:16:42.66 AFV.select :carrierSolution
@14:16:42.76 delay 2 seconds
@14:16:44.79 AS.reconfigure ASconfig
@14:16:44.85 AS.seek 1ml
@14:17:42.58 delay 1 second
@14:17:43.60 AFV.select :holdingCoil
@14:17:43.70 delay 1 second
@14:17:44.72 AS.reconfigure ASfast
@14:17:44.78 AS.seek 0ml
@14:18:12.11 delay 3 seconds
@14:18:15.13 AFV.select :carrierSolution
@14:18:15.23 delay 2 seconds
@14:18:17.26 AS.reconfigure ASconfig
@14:18:17.32 AS.seek 1ml
@14:19:15.05 delay 1 second
@14:19:16.07 AFV.select :holdingCoil
@14:19:16.17 delay 1 second
@14:19:17.19 AS.reconfigure ASnorm
@14:19:17.25 AS.seek 0ml
@14:20:07.94 Setting up PCR for ACYPP2 -- writing /var/log/esp/ACYPP221apr2714h.pcr
@14:20:07.96 Building PCR Slug from ASV with MM1 mix, PP2 primer
@14:20:07.98 Priming with carrier
@14:20:08.00 ATV.dial ARV
@14:20:08.75 ARV.dial :carrier
@14:20:09.11 AS.seek 0.5ml
@14:20:34.69 AFV.select :carrierSolution
@14:20:34.80 delay 2 seconds
@14:20:36.83 AS.reconfigure ASconfig
@14:20:36.88 AS.seek 1ml
@14:21:05.89 delay 1 second
@14:21:06.91 AFV.select :holdingCoil
@14:21:07.01 delay 1 second
@14:21:08.03 ATV.dial :waste
@14:21:09.81 AS.reconfigure ASnorm
@14:21:09.87 AS.seek 0ml
@14:22:00.58 AFV.select :carrierSolution
@14:22:00.69 delay 2 seconds
@14:22:02.72 AS.reconfigure ASconfig
@14:22:02.77 AS.seek 0.5ml
@14:22:31.83 delay 1 second
@14:22:32.85 AFV.select :holdingCoil
@14:22:32.95 Building Slug
@14:22:32.97 ATV.dial :ARV
@14:22:34.72 delay 2 seconds
@14:22:36.74 ARV.dial :air1
@14:22:37.17 delay 2 seconds
@14:22:39.24 AS.reconfigure ASslow2
@14:22:39.30 AS.seek 0.506ml
@14:22:45.52 delay 2 seconds
@14:22:47.54 ARV.dial :MM1
@14:22:48.17 delay 2 seconds
@14:22:50.19 Adding MM1
@14:22:50.70 AS.seek 0.524ml
@14:23:09.11 delay 25 seconds
@14:23:34.13 ARV.dial :PP2
@14:23:34.55 Adding PP2
@14:23:34.56 delay 2 seconds
@14:23:36.64 AS.seek 0.53ml
@14:23:43.28 delay 10 seconds
@14:23:53.31 ARV.dial :air2
@14:23:53.75 adding ARV air
@14:23:53.76 delay 2 seconds
@14:23:55.84 AS.seek 0.536ml
@14:24:02.64 delay 5 seconds
@14:24:07.66 ARV.dial :carrier
@14:24:09.03 delay 7 seconds
@14:24:16.11 AS.seek 0.594ml
@14:25:14.66 delay 5 seconds
@14:25:19.68 ATV.dial :ASV
@14:25:20.11 delay 2 seconds
@14:25:22.18 AS.seek 0.6ml
@14:25:28.49 delay 4 seconds
@14:25:32.51 ATV.dial ARV
@14:25:33.11 delay 2 seconds
@14:25:35.13 ARV.dial :carrier
@14:25:35.14 delay 2 seconds
@14:25:37.22 AS.seek 0.65ml
@14:26:27.99 delay 4 seconds
@14:26:32.01 ATV.dial PCR
@14:26:32.67 ASV.dial :waste1
@14:26:32.68 ARV.dial :air1
@14:26:33.12 Positioning Slug by 0.324ml
@14:26:33.21 AS.seek 0.326ml
@14:31:59.60 delay 5 seconds
@14:32:04.62 ATV.dial ARV
@14:32:05.44 ASV.dial :PCRlock
@14:32:05.85 delay 5 seconds
@14:32:10.87 Running ACYPP2 Profile60CIQ2 thermocycling
@14:32:10.89 <PCR> PCR.program Profile60CIQ2
@14:32:11.01 Profile60CIQ2 should complete in < 1:47:50
@14:32:11.02 PCR.run 2:19:47.5
@16:00:04.45 PCR.off
@16:00:05.07 <MFBPCR> Running ACYPP2 MeltProfile60 thermocycling
@16:00:05.08 <PCR> PCR.program MeltProfile60
@16:00:05.18 MeltProfile60 should complete in < 11:55
@16:00:05.19 PCR.run 19:53.75
@16:07:30.72 PCR.off
@16:07:31.65 <MFBPCR> PCR for ACYPP2 completed -- closed /var/log/esp/ACYPP221apr2714h.pcr
@16:07:31.72 Cleaning PCR
@16:07:31.73 AFV.select :carrierSolution
@16:07:31.85 delay 2 seconds
@16:07:33.88 AS.reconfigure ASconfig
@16:07:33.93 AS.seek 0.5ml
@16:07:44.24 delay 1 second
@16:07:45.26 AFV.select :holdingCoil
@16:07:45.36 ASV.dial :waste1
@16:07:45.76 ATV.dial :air3
@16:07:47.12 AS.reconfigure ASslow1
@16:07:47.18 AS.seek 0.51ml
@16:07:49.47 delay 2 seconds
@16:07:51.49 ATV.dial :bleach
@16:07:52.16 AS.seek 0.81ml
@16:08:52.76 delay 3 seconds
@16:08:55.78 ATV.dial :air3
@16:08:56.36 delay 3 seconds
@16:08:59.43 AS.seek 0.835ml
@16:09:04.74 delay 3 seconds
@16:09:07.76 ATV.dial :bleach
@16:09:08.41 AS.seek 0.82ml
@16:09:11.83 delay 3 seconds
@16:09:14.85 ARV.dial :waste
@16:09:15.51 delay 3 seconds
@16:09:18.53 ATV.dial :ARV
@16:09:19.46 AS.reconfigure ASconfig
@16:09:19.52 AS.seek 0.47ml
@16:09:39.94 AFV.select :carrierSolution
@16:09:40.04 delay 2 seconds
@16:09:42.07 AS.seek 1ml
@16:10:12.83 delay 1 second
@16:10:13.85 AFV.select :holdingCoil
@16:10:13.95 delay 1 second
@16:10:14.97 AS.reconfigure ASfast
@16:10:15.03 AS.seek 0ml
@16:10:42.36 AFV.select :carrierSolution
@16:10:42.46 delay 2 seconds
@16:10:44.48 AS.reconfigure ASconfig
@16:10:44.94 AS.seek 1ml
@16:11:42.74 delay 1 second
@16:11:43.76 AFV.select :holdingCoil
@16:11:43.86 delay 1 second
@16:11:44.89 AS.reconfigure ASfast
@16:11:44.94 AS.seek 0ml
@16:12:12.27 AFV.select :carrierSolution
@16:12:12.38 delay 2 seconds
@16:12:14.41 AS.reconfigure ASconfig
@16:12:14.46 AS.seek 1ml
@16:13:12.21 delay 1 second
@16:13:13.23 AFV.select :holdingCoil
@16:13:13.33 delay 1 second
@16:13:14.36 AS.reconfigure ASfast
@16:13:14.41 AS.seek 0ml
@16:13:41.76 ARV.dial between carrier and air1
@16:13:42.27 ATV.dial :air3
@16:13:43.64 AS.reconfigure ASslow1
@16:13:43.70 AS.seek 0.01ml
@16:13:46.05 delay 2 seconds
@16:13:48.07 ATV.dial :bleach
@16:13:48.72 AS.seek 0.81ml
@16:16:29.91 delay 3 seconds
@16:16:32.93 ATV.dial :air3
@16:16:33.52 delay 3 seconds
@16:16:36.59 AS.seek 0.835ml
@16:16:41.95 delay 3 seconds
@16:16:44.97 ATV.dial :bleach
@16:16:45.63 AS.seek 0.82ml
@16:16:48.94 delay 3 seconds
@16:16:51.96 ATV.dial PCR
@16:16:52.40 AS.reconfigure ASconfig
@16:16:52.46 AS.seek 0ml
@16:17:39.88 PCR.program :bleachProfile
@16:17:39.95 PCR program should complete in < 2:45
@16:17:39.96 PCR.run 10:00
@16:20:38.78 PCR.off
@16:20:38.82 AFV.select :carrierSolution
@16:20:38.93 delay 2 seconds
@16:20:40.96 AS.seek 1ml
@16:21:38.71 delay 1 second
@16:21:39.73 AFV.select :holdingCoil
@16:21:39.83 delay 1 second
@16:21:40.85 AS.reconfigure ASfast
@16:21:40.91 AS.seek 0ml
@16:22:08.24 delay 3 seconds
@16:22:11.26 AFV.select :carrierSolution
@16:22:11.36 delay 2 seconds
@16:22:13.38 AS.reconfigure ASconfig
@16:22:13.45 AS.seek 1ml
@16:23:11.19 delay 1 second
@16:23:12.21 AFV.select :holdingCoil
@16:23:12.31 delay 1 second
@16:23:13.33 AS.reconfigure ASfast
@16:23:13.39 AS.seek 0ml
@16:23:40.72 delay 3 seconds
@16:23:43.74 AFV.select :carrierSolution
@16:23:43.84 delay 2 seconds
@16:23:45.86 AS.reconfigure ASconfig
@16:23:45.93 AS.seek 1ml
@16:24:43.68 delay 1 second
@16:24:44.70 AFV.select :holdingCoil
@16:24:44.80 delay 1 second
@16:24:45.83 AS.reconfigure ASfast
@16:24:46.29 AS.seek 0ml
@16:25:13.63 delay 3 seconds
@16:25:16.65 AFV.select :carrierSolution
@16:25:16.75 delay 2 seconds
@16:25:18.78 AS.reconfigure ASconfig
@16:25:18.84 AS.seek 1ml
@16:26:16.62 delay 1 second
@16:26:17.64 AFV.select :holdingCoil
@16:26:17.74 delay 1 second
@16:26:18.77 AS.reconfigure ASfast
@16:26:18.82 AS.seek 0ml
@16:26:46.16 delay 3 seconds
@16:26:49.18 AFV.select :carrierSolution
@16:26:49.28 delay 2 seconds
@16:26:51.30 AS.reconfigure ASconfig
@16:26:51.37 AS.seek 1ml
@16:27:49.12 delay 1 second
@16:27:50.14 AFV.select :holdingCoil
@16:27:50.24 delay 1 second
@16:27:51.27 AS.reconfigure ASnorm
@16:27:51.32 AS.seek 0ml
@16:28:41.99 Setting up PCR for AOPP1 -- writing /var/log/esp/AOPP121apr2716h.pcr
@16:28:42.01 Building PCR Slug from ASV with MM1 mix, PP1 primer
@16:28:42.03 Priming with carrier
@16:28:42.05 ATV.dial ARV
@16:28:42.71 ARV.dial :carrier
@16:28:43.05 AS.seek 0.5ml
@16:29:08.58 AFV.select :carrierSolution
@16:29:08.69 delay 2 seconds
@16:29:10.72 AS.reconfigure ASconfig
@16:29:10.78 AS.seek 1ml
@16:29:39.80 delay 1 second
@16:29:40.82 AFV.select :holdingCoil
@16:29:40.92 delay 1 second
@16:29:41.94 ATV.dial :waste
@16:29:43.72 AS.reconfigure ASnorm
@16:29:43.79 AS.seek 0ml
@16:30:34.47 AFV.select :carrierSolution
@16:30:34.58 delay 2 seconds
@16:30:36.61 AS.reconfigure ASconfig
@16:30:36.66 AS.seek 0.5ml
@16:31:05.71 delay 1 second
@16:31:06.73 AFV.select :holdingCoil
@16:31:06.83 Building Slug
@16:31:06.84 ATV.dial :ARV
@16:31:08.60 delay 2 seconds
@16:31:10.62 ARV.dial :air1
@16:31:11.03 delay 2 seconds
@16:31:13.10 AS.reconfigure ASslow2
@16:31:13.16 AS.seek 0.506ml
@16:31:19.87 delay 2 seconds
@16:31:21.89 ARV.dial :MM1
@16:31:22.54 delay 2 seconds
@16:31:24.56 Adding MM1
@16:31:24.64 AS.seek 0.524ml
@16:31:43.22 delay 25 seconds
@16:32:08.24 ARV.dial :PP1
@16:32:08.66 Adding PP1
@16:32:08.67 delay 2 seconds
@16:32:10.75 AS.seek 0.53ml
@16:32:17.54 delay 10 seconds
@16:32:27.57 ARV.dial :air1
@16:32:27.97 adding ARV air
@16:32:27.98 delay 2 seconds
@16:32:30.06 AS.seek 0.536ml
@16:32:36.38 delay 5 seconds
@16:32:41.40 ARV.dial :carrier
@16:32:41.83 delay 7 seconds
@16:32:48.91 AS.seek 0.594ml
@16:33:47.55 delay 5 seconds
@16:33:52.57 ATV.dial :ASV
@16:33:53.01 delay 2 seconds
@16:33:55.08 AS.seek 0.6ml
@16:34:01.44 delay 4 seconds
@16:34:05.46 ATV.dial ARV
@16:34:06.05 delay 2 seconds
@16:34:08.07 ARV.dial :carrier
@16:34:08.08 delay 2 seconds
@16:34:10.16 AS.seek 0.65ml
@16:35:00.85 delay 4 seconds
@16:35:04.87 ATV.dial PCR
@16:35:05.51 ASV.dial :waste1
@16:35:05.52 ARV.dial :air1
@16:35:05.95 Positioning Slug by 0.324ml
@16:35:06.04 AS.seek 0.326ml
@16:40:32.13 delay 5 seconds
@16:40:37.15 ATV.dial ARV
@16:40:37.97 ASV.dial :PCRlock
@16:40:38.38 delay 5 seconds
@16:40:43.40 Running AOPP1 Profile60CIQ1 thermocycling
@16:40:43.42 <PCR> PCR.program Profile60CIQ1
@16:40:43.53 Profile60CIQ1 should complete in < 1:56:15
@16:40:43.54 PCR.run 2:30:18.75
@18:19:21.08 PCR.off
@18:19:21.65 <MFBPCR> Running AOPP1 MeltProfile60 thermocycling
@18:19:21.66 <PCR> PCR.program MeltProfile60
@18:19:21.76 MeltProfile60 should complete in < 11:55
@18:19:21.77 PCR.run 19:53.75
@18:26:50.22 PCR.off
@18:26:51.14 <MFBPCR> PCR for AOPP1 completed -- closed /var/log/esp/AOPP121apr2716h.pcr
@18:26:51.21 Cleaning PCR
@18:26:51.22 AFV.select :carrierSolution
@18:26:51.34 delay 2 seconds
@18:26:53.37 AS.reconfigure ASconfig
@18:26:53.42 AS.seek 0.5ml
@18:27:03.74 delay 1 second
@18:27:04.76 AFV.select :holdingCoil
@18:27:04.86 ASV.dial :waste1
@18:27:05.26 ATV.dial :air3
@18:27:06.62 AS.reconfigure ASslow1
@18:27:06.68 AS.seek 0.51ml
@18:27:08.97 delay 2 seconds
@18:27:10.99 ATV.dial :bleach
@18:27:11.65 AS.seek 0.81ml
@18:28:12.24 delay 3 seconds
@18:28:15.26 ATV.dial :air3
@18:28:15.85 delay 3 seconds
@18:28:18.92 AS.seek 0.835ml
@18:28:24.22 delay 3 seconds
@18:28:27.24 ATV.dial :bleach
@18:28:27.87 AS.seek 0.82ml
@18:28:31.16 delay 3 seconds
@18:28:34.18 ARV.dial :waste
@18:28:34.84 delay 3 seconds
@18:28:38.24 ATV.dial :ARV
@18:28:39.18 AS.reconfigure ASconfig
@18:28:39.24 AS.seek 0.47ml
@18:28:59.66 AFV.select :carrierSolution
@18:28:59.77 delay 2 seconds
@18:29:01.80 AS.seek 1ml
@18:29:32.57 delay 1 second
@18:29:33.59 AFV.select :holdingCoil
@18:29:33.69 delay 1 second
@18:29:34.72 AS.reconfigure ASfast
@18:29:34.77 AS.seek 0ml
@18:30:02.12 AFV.select :carrierSolution
@18:30:02.23 delay 2 seconds
@18:30:04.26 AS.reconfigure ASconfig
@18:30:04.31 AS.seek 1ml
@18:31:02.05 delay 1 second
@18:31:03.07 AFV.select :holdingCoil
@18:31:03.17 delay 1 second
@18:31:04.20 AS.reconfigure ASfast
@18:31:04.25 AS.seek 0ml
@18:31:31.55 AFV.select :carrierSolution
@18:31:31.66 delay 2 seconds
@18:31:33.68 AS.reconfigure ASconfig
@18:31:33.74 AS.seek 1ml
@18:32:31.49 delay 1 second
@18:32:32.51 AFV.select :holdingCoil
@18:32:32.61 delay 1 second
@18:32:33.64 AS.reconfigure ASfast
@18:32:33.69 AS.seek 0ml
@18:33:01.02 ARV.dial between carrier and air1
@18:33:01.56 ATV.dial :air3
@18:33:02.91 AS.reconfigure ASslow1
@18:33:02.97 AS.seek 0.01ml
@18:33:05.45 delay 2 seconds
@18:33:07.47 ATV.dial :bleach
@18:33:08.12 AS.seek 0.81ml
@18:35:49.31 delay 3 seconds
@18:35:52.33 ATV.dial :air3
@18:35:52.91 delay 3 seconds
@18:35:55.98 AS.seek 0.835ml
@18:36:01.27 delay 3 seconds
@18:36:04.29 ATV.dial :bleach
@18:36:04.93 AS.seek 0.82ml
@18:36:08.26 delay 3 seconds
@18:36:11.28 ATV.dial PCR
@18:36:11.71 AS.reconfigure ASconfig
@18:36:11.77 AS.seek 0ml
@18:36:59.16 PCR.program :bleachProfile
@18:36:59.23 PCR program should complete in < 2:45
@18:36:59.24 PCR.run 10:00
@18:39:55.03 PCR.off
@18:39:55.06 AFV.select :carrierSolution
@18:39:55.18 delay 2 seconds
@18:39:57.21 AS.seek 1ml
@18:40:54.93 delay 1 second
@18:40:55.95 AFV.select :holdingCoil
@18:40:56.05 delay 1 second
@18:40:57.07 AS.reconfigure ASfast
@18:40:57.13 AS.seek 0ml
@18:41:24.48 delay 3 seconds
@18:41:27.50 AFV.select :carrierSolution
@18:41:27.60 delay 2 seconds
@18:41:29.99 AS.reconfigure ASconfig
@18:41:30.11 AS.seek 1ml
@18:42:27.87 delay 1 second
@18:42:28.89 AFV.select :holdingCoil
@18:42:28.99 delay 1 second
@18:42:30.02 AS.reconfigure ASfast
@18:42:30.07 AS.seek 0ml
@18:42:57.41 delay 3 seconds
@18:43:00.43 AFV.select :carrierSolution
@18:43:00.53 delay 2 seconds
@18:43:02.56 AS.reconfigure ASconfig
@18:43:02.61 AS.seek 1ml
@18:44:00.35 delay 1 second
@18:44:01.37 AFV.select :holdingCoil
@18:44:01.47 delay 1 second
@18:44:02.50 AS.reconfigure ASfast
@18:44:02.55 AS.seek 0ml
@18:44:29.90 delay 3 seconds
@18:44:32.92 AFV.select :carrierSolution
@18:44:33.02 delay 2 seconds
@18:44:35.05 AS.reconfigure ASconfig
@18:44:35.11 AS.seek 1ml
@18:45:32.85 delay 1 second
@18:45:33.87 AFV.select :holdingCoil
@18:45:33.97 delay 1 second
@18:45:35.00 AS.reconfigure ASfast
@18:45:35.05 AS.seek 0ml
@18:46:02.40 delay 3 seconds
@18:46:05.42 AFV.select :carrierSolution
@18:46:05.52 delay 2 seconds
@18:46:07.54 AS.reconfigure ASconfig
@18:46:07.60 AS.seek 1ml
@18:47:05.37 delay 1 second
@18:47:06.39 AFV.select :holdingCoil
@18:47:06.49 delay 1 second
@18:47:07.52 AS.reconfigure ASnorm
@18:47:07.57 AS.seek 0ml
@18:47:58.34 Setting up PCR for AARPP4 -- writing /var/log/esp/AARPP421apr2718h.pcr
@18:47:58.36 Building PCR Slug from ASV with MM2 mix, PP4 primer
@18:47:58.38 Priming with carrier
@18:47:58.40 ATV.dial ARV
@18:47:59.06 ARV.dial :carrier
@18:47:59.41 AS.seek 0.5ml
@18:48:24.96 AFV.select :carrierSolution
@18:48:25.08 delay 2 seconds
@18:48:27.12 AS.reconfigure ASconfig
@18:48:27.18 AS.seek 1ml
@18:48:56.18 delay 1 second
@18:48:57.20 AFV.select :holdingCoil
@18:48:57.30 delay 1 second
@18:48:58.32 ATV.dial :waste
@18:49:00.10 AS.reconfigure ASnorm
@18:49:00.16 AS.seek 0ml
@18:49:50.87 AFV.select :carrierSolution
@18:49:50.98 delay 2 seconds
@18:49:53.01 AS.reconfigure ASconfig
@18:49:53.06 AS.seek 0.5ml
@18:50:22.13 delay 1 second
@18:50:23.15 AFV.select :holdingCoil
@18:50:23.25 Building Slug
@18:50:23.26 ATV.dial :ARV
@18:50:25.02 delay 2 seconds
@18:50:27.04 ARV.dial :air1
@18:50:27.47 delay 2 seconds
@18:50:29.54 AS.reconfigure ASslow2
@18:50:29.60 AS.seek 0.506ml
@18:50:35.99 delay 2 seconds
@18:50:38.01 ARV.dial :MM2
@18:50:39.60 delay 2 seconds
@18:50:41.62 Adding MM2
@18:50:41.70 AS.seek 0.524ml
@18:51:00.24 delay 25 seconds
@18:51:25.26 ARV.dial :PP4
@18:51:25.68 Adding PP4
@18:51:25.69 delay 2 seconds
@18:51:27.77 AS.seek 0.53ml
@18:51:34.16 delay 10 seconds
@18:51:44.19 ARV.dial :air3
@18:51:44.60 adding ARV air
@18:51:44.61 delay 2 seconds
@18:51:46.69 AS.seek 0.536ml
@18:51:53.40 delay 5 seconds
@18:51:58.42 ARV.dial :carrier
@18:51:59.77 delay 7 seconds
@18:52:06.85 AS.seek 0.594ml
@18:53:05.65 delay 5 seconds
@18:53:10.67 ATV.dial :ASV
@18:53:11.10 delay 2 seconds
@18:53:13.17 AS.seek 0.6ml
@18:53:19.60 delay 4 seconds
@18:53:23.62 ATV.dial ARV
@18:53:24.21 delay 2 seconds
@18:53:26.23 ARV.dial :carrier
@18:53:26.24 delay 2 seconds
@18:53:28.32 AS.seek 0.65ml
@18:54:19.02 delay 4 seconds
@18:54:23.04 ATV.dial PCR
@18:54:23.70 ASV.dial :waste1
@18:54:23.71 ARV.dial :air1
@18:54:24.13 Positioning Slug by 0.324ml
@18:54:24.21 AS.seek 0.326ml
@18:59:50.49 delay 5 seconds
@18:59:55.51 ATV.dial ARV
@18:59:56.32 ASV.dial :PCRlock
@18:59:56.73 delay 5 seconds
@19:00:01.75 Running AARPP4 Profile58CIQ2 thermocycling
@19:00:01.76 <PCR> PCR.program Profile58CIQ2
@19:00:01.89 Profile58CIQ2 should complete in < 1:36:15
@19:00:01.90 PCR.run 2:05:18.75
@20:20:37.90 PCR.off
@20:20:38.45 <MFBPCR> Running AARPP4 MeltProfile58 thermocycling
@20:20:38.47 <PCR> PCR.program MeltProfile58
@20:20:38.58 MeltProfile58 should complete in < 12:33
@20:20:38.59 PCR.run 20:41.25
@20:28:23.40 PCR.off
@20:28:24.55 <MFBPCR> PCR for AARPP4 completed -- closed /var/log/esp/AARPP421apr2718h.pcr
@20:28:24.63 Cleaning PCR
@20:28:24.64 AFV.select :carrierSolution
@20:28:24.76 delay 2 seconds
@20:28:26.79 AS.reconfigure ASconfig
@20:28:26.84 AS.seek 0.5ml
@20:28:37.14 delay 1 second
@20:28:38.16 AFV.select :holdingCoil
@20:28:38.26 ASV.dial :waste1
@20:28:38.65 ATV.dial :air3
@20:28:40.01 AS.reconfigure ASslow1
@20:28:40.07 AS.seek 0.51ml
@20:28:42.38 delay 2 seconds
@20:28:44.40 ATV.dial :bleach
@20:28:45.07 AS.seek 0.81ml
@20:29:45.68 delay 3 seconds
@20:29:48.70 ATV.dial :air3
@20:29:49.29 delay 3 seconds
@20:29:52.36 AS.seek 0.835ml
@20:29:57.64 delay 3 seconds
@20:30:00.66 ATV.dial :bleach
@20:30:01.31 AS.seek 0.82ml
@20:30:04.62 delay 3 seconds
@20:30:07.64 ARV.dial :waste
@20:30:08.29 delay 3 seconds
@20:30:11.31 ATV.dial :ARV
@20:30:12.25 AS.reconfigure ASconfig
@20:30:12.31 AS.seek 0.47ml
@20:30:32.71 AFV.select :carrierSolution
@20:30:32.82 delay 2 seconds
@20:30:34.85 AS.seek 1ml
@20:31:05.59 delay 1 second
@20:31:06.61 AFV.select :holdingCoil
@20:31:06.71 delay 1 second
@20:31:07.74 AS.reconfigure ASfast
@20:31:07.79 AS.seek 0ml
@20:31:35.15 AFV.select :carrierSolution
@20:31:35.26 delay 2 seconds
@20:31:37.28 AS.reconfigure ASconfig
@20:31:37.34 AS.seek 1ml
@20:32:35.09 delay 1 second
@20:32:36.11 AFV.select :holdingCoil
@20:32:36.21 delay 1 second
@20:32:37.24 AS.reconfigure ASfast
@20:32:37.29 AS.seek 0ml
@20:33:04.64 AFV.select :carrierSolution
@20:33:04.75 delay 2 seconds
@20:33:06.77 AS.reconfigure ASconfig
@20:33:06.83 AS.seek 1ml
@20:34:04.59 delay 1 second
@20:34:05.61 AFV.select :holdingCoil
@20:34:05.71 delay 1 second
@20:34:06.74 AS.reconfigure ASfast
@20:34:06.79 AS.seek 0ml
@20:34:34.14 ARV.dial between carrier and air1
@20:34:34.67 ATV.dial :air3
@20:34:36.03 AS.reconfigure ASslow1
@20:34:36.10 AS.seek 0.01ml
@20:34:38.57 delay 2 seconds
@20:34:40.59 ATV.dial :bleach
@20:34:41.23 AS.seek 0.81ml
@20:37:22.45 delay 3 seconds
@20:37:25.47 ATV.dial :air3
@20:37:26.06 delay 3 seconds
@20:37:29.13 AS.seek 0.835ml
@20:37:34.47 delay 3 seconds
@20:37:37.49 ATV.dial :bleach
@20:37:38.13 AS.seek 0.82ml
@20:37:41.64 delay 3 seconds
@20:37:44.66 ATV.dial PCR
@20:37:45.09 AS.reconfigure ASconfig
@20:37:45.15 AS.seek 0ml
@20:38:32.57 PCR.program :bleachProfile
@20:38:32.64 PCR program should complete in < 2:45
@20:38:32.65 PCR.run 10:00
@20:41:28.86 PCR.off
@20:41:28.90 AFV.select :carrierSolution
@20:41:29.01 delay 2 seconds
@20:41:31.04 AS.seek 1ml
@20:42:28.76 delay 1 second
@20:42:29.78 AFV.select :holdingCoil
@20:42:29.88 delay 1 second
@20:42:30.91 AS.reconfigure ASfast
@20:42:30.96 AS.seek 0ml
@20:42:58.28 delay 3 seconds
@20:43:01.30 AFV.select :carrierSolution
@20:43:01.40 delay 2 seconds
@20:43:03.42 AS.reconfigure ASconfig
@20:43:03.49 AS.seek 1ml
@20:44:01.28 delay 1 second
@20:44:02.30 AFV.select :holdingCoil
@20:44:02.40 delay 1 second
@20:44:03.43 AS.reconfigure ASfast
@20:44:03.48 AS.seek 0ml
@20:44:30.82 delay 3 seconds
@20:44:33.84 AFV.select :carrierSolution
@20:44:33.94 delay 2 seconds
@20:44:35.97 AS.reconfigure ASconfig
@20:44:36.03 AS.seek 1ml
@20:45:33.78 delay 1 second
@20:45:34.80 AFV.select :holdingCoil
@20:45:34.90 delay 1 second
@20:45:35.93 AS.reconfigure ASfast
@20:45:35.98 AS.seek 0ml
@20:46:03.32 delay 3 seconds
@20:46:06.34 AFV.select :carrierSolution
@20:46:06.44 delay 2 seconds
@20:46:08.46 AS.reconfigure ASconfig
@20:46:08.52 AS.seek 1ml
@20:47:06.28 delay 1 second
@20:47:07.30 AFV.select :holdingCoil
@20:47:07.40 delay 1 second
@20:47:08.43 AS.reconfigure ASfast
@20:47:08.48 AS.seek 0ml
@20:47:35.82 delay 3 seconds
@20:47:38.84 AFV.select :carrierSolution
@20:47:38.94 delay 2 seconds
@20:47:40.96 AS.reconfigure ASconfig
@20:47:41.03 AS.seek 1ml
@20:48:38.81 delay 1 second
@20:48:39.83 AFV.select :holdingCoil
@20:48:39.94 delay 1 second
@20:48:40.97 AS.reconfigure ASnorm
@20:48:41.02 AS.seek 0ml
@20:49:31.70 Setting up PCR for AALPP3 -- writing /var/log/esp/AALPP321apr2720h.pcr
@20:49:31.72 Building PCR Slug from ASV with MM2 mix, PP3 primer
@20:49:31.74 Priming with carrier
@20:49:31.76 ATV.dial ARV
@20:49:32.42 ARV.dial :carrier
@20:49:32.77 AS.seek 0.5ml
@20:49:58.29 AFV.select :carrierSolution
@20:49:58.41 delay 2 seconds
@20:50:00.44 AS.reconfigure ASconfig
@20:50:00.50 AS.seek 1ml
@20:50:29.53 delay 1 second
@20:50:30.55 AFV.select :holdingCoil
@20:50:30.65 delay 1 second
@20:50:31.67 ATV.dial :waste
@20:50:33.45 AS.reconfigure ASnorm
@20:50:33.51 AS.seek 0ml
@20:51:24.20 AFV.select :carrierSolution
@20:51:24.32 delay 2 seconds
@20:51:26.35 AS.reconfigure ASconfig
@20:51:26.40 AS.seek 0.5ml
@20:51:55.46 delay 1 second
@20:51:56.48 AFV.select :holdingCoil
@20:51:56.58 Building Slug
@20:51:56.59 ATV.dial :ARV
@20:51:58.34 delay 2 seconds
@20:52:00.36 ARV.dial :air1
@20:52:00.77 delay 2 seconds
@20:52:02.84 AS.reconfigure ASslow2
@20:52:02.90 AS.seek 0.506ml
@20:52:09.35 delay 2 seconds
@20:52:11.37 ARV.dial :MM2
@20:52:12.98 delay 2 seconds
@20:52:15.00 Adding MM2
@20:52:15.07 AS.seek 0.524ml
@20:52:33.50 delay 25 seconds
@20:52:58.52 ARV.dial :PP3
@20:52:58.93 Adding PP3
@20:52:58.94 delay 2 seconds
@20:53:01.02 AS.seek 0.53ml
@20:53:07.31 delay 10 seconds
@20:53:17.34 ARV.dial :air2
@20:53:17.76 adding ARV air
@20:53:17.77 delay 2 seconds
@20:53:19.85 AS.seek 0.536ml
@20:53:26.43 delay 5 seconds
@20:53:31.45 ARV.dial :carrier
@20:53:32.81 delay 7 seconds
@20:53:39.88 AS.seek 0.594ml
@20:54:38.76 delay 5 seconds
@20:54:43.78 ATV.dial :ASV
@20:54:44.21 delay 2 seconds
@20:54:46.28 AS.seek 0.6ml
@20:54:52.57 delay 4 seconds
@20:54:56.59 ATV.dial ARV
@20:54:57.18 delay 2 seconds
@20:54:59.20 ARV.dial :carrier
@20:54:59.62 delay 2 seconds
@20:55:01.71 AS.seek 0.65ml
@20:55:52.29 delay 4 seconds
@20:55:56.31 ATV.dial PCR
@20:55:56.95 ASV.dial :waste1
@20:55:56.96 ARV.dial :air1
@20:55:57.40 Positioning Slug by 0.324ml
@20:55:57.49 AS.seek 0.326ml
@21:01:23.28 delay 5 seconds
@21:01:28.30 ATV.dial ARV
@21:01:29.12 ASV.dial :PCRlock
@21:01:29.53 delay 5 seconds
@21:01:34.55 Running AALPP3 Profile58CIQ2 thermocycling
@21:01:34.59 <PCR> PCR.program Profile58CIQ2
@21:01:34.78 Profile58CIQ2 should complete in < 1:36:15
@21:01:34.81 PCR.run 2:05:18.75
@22:22:09.95 PCR.off
@22:22:10.89 <MFBPCR> Running AALPP3 MeltProfile58 thermocycling
@22:22:10.91 <PCR> PCR.program MeltProfile58
@22:22:11.01 MeltProfile58 should complete in < 12:33
@22:22:11.02 PCR.run 20:41.25
@22:29:53.19 PCR.off
@22:29:54.18 <MFBPCR> PCR for AALPP3 completed -- closed /var/log/esp/AALPP321apr2720h.pcr
@22:29:54.25 Cleaning PCR
@22:29:54.26 AFV.select :carrierSolution
@22:29:54.38 delay 2 seconds
@22:29:56.41 AS.reconfigure ASconfig
@22:29:56.46 AS.seek 0.5ml
@22:30:06.78 delay 1 second
@22:30:07.80 AFV.select :holdingCoil
@22:30:07.90 ASV.dial :waste1
@22:30:08.29 ATV.dial :air3
@22:30:09.64 AS.reconfigure ASslow1
@22:30:09.70 AS.seek 0.51ml
@22:30:11.99 delay 2 seconds
@22:30:14.01 ATV.dial :bleach
@22:30:14.67 AS.seek 0.81ml
@22:31:15.34 delay 3 seconds
@22:31:18.36 ATV.dial :air3
@22:31:18.95 delay 3 seconds
@22:31:22.02 AS.seek 0.835ml
@22:31:27.34 delay 3 seconds
@22:31:30.36 ATV.dial :bleach
@22:31:31.01 AS.seek 0.82ml
@22:31:34.31 delay 3 seconds
@22:31:37.33 ARV.dial :waste
@22:31:37.98 delay 3 seconds
@22:31:41.00 ATV.dial :ARV
@22:31:41.94 AS.reconfigure ASconfig
@22:31:42.00 AS.seek 0.47ml
@22:32:02.40 AFV.select :carrierSolution
@22:32:02.51 delay 2 seconds
@22:32:04.54 AS.seek 1ml
@22:32:35.33 delay 1 second
@22:32:36.35 AFV.select :holdingCoil
@22:32:36.45 delay 1 second
@22:32:37.47 AS.reconfigure ASfast
@22:32:37.53 AS.seek 0ml
@22:33:04.87 AFV.select :carrierSolution
@22:33:04.98 delay 2 seconds
@22:33:07.01 AS.reconfigure ASconfig
@22:33:07.06 AS.seek 1ml
@22:34:04.81 delay 1 second
@22:34:05.83 AFV.select :holdingCoil
@22:34:05.93 delay 1 second
@22:34:06.96 AS.reconfigure ASfast
@22:34:07.01 AS.seek 0ml
@22:34:34.33 AFV.select :carrierSolution
@22:34:34.43 delay 2 seconds
@22:34:36.46 AS.reconfigure ASconfig
@22:34:36.51 AS.seek 1ml
@22:35:34.23 delay 1 second
@22:35:35.25 AFV.select :holdingCoil
@22:35:35.35 delay 1 second
@22:35:36.37 AS.reconfigure ASfast
@22:35:36.43 AS.seek 0ml
@22:36:03.76 ARV.dial between carrier and air1
@22:36:04.28 ATV.dial :air3
@22:36:05.63 AS.reconfigure ASslow1
@22:36:05.69 AS.seek 0.01ml
@22:36:08.01 delay 2 seconds
@22:36:10.03 ATV.dial :bleach
@22:36:10.68 AS.seek 0.81ml
@22:38:51.83 delay 3 seconds
@22:38:54.85 ATV.dial :air3
@22:38:55.43 delay 3 seconds
@22:38:58.50 AS.seek 0.835ml
@22:39:03.84 delay 3 seconds
@22:39:06.86 ATV.dial :bleach
@22:39:07.50 AS.seek 0.82ml
@22:39:10.75 delay 3 seconds
@22:39:13.77 ATV.dial PCR
@22:39:14.20 AS.reconfigure ASconfig
@22:39:14.26 AS.seek 0ml
@22:40:01.67 PCR.program :bleachProfile
@22:40:01.74 PCR program should complete in < 2:45
@22:40:01.75 PCR.run 10:00
@22:42:57.63 PCR.off
@22:42:57.66 AFV.select :carrierSolution
@22:42:57.78 delay 2 seconds
@22:42:59.81 AS.seek 1ml
@22:43:57.59 delay 1 second
@22:43:58.61 AFV.select :holdingCoil
@22:43:58.71 delay 1 second
@22:43:59.74 AS.reconfigure ASfast
@22:43:59.79 AS.seek 0ml
@22:44:27.12 delay 3 seconds
@22:44:30.14 AFV.select :carrierSolution
@22:44:30.24 delay 2 seconds
@22:44:32.26 AS.reconfigure ASconfig
@22:44:32.33 AS.seek 1ml
@22:45:30.12 delay 1 second
@22:45:31.14 AFV.select :holdingCoil
@22:45:31.24 delay 1 second
@22:45:32.27 AS.reconfigure ASfast
@22:45:32.32 AS.seek 0ml
@22:45:59.65 delay 3 seconds
@22:46:02.67 AFV.select :carrierSolution
@22:46:02.77 delay 2 seconds
@22:46:04.79 AS.reconfigure ASconfig
@22:46:04.85 AS.seek 1ml
@22:47:02.64 delay 1 second
@22:47:03.66 AFV.select :holdingCoil
@22:47:03.76 delay 1 second
@22:47:04.79 AS.reconfigure ASfast
@22:47:04.84 AS.seek 0ml
@22:47:32.20 delay 3 seconds
@22:47:35.22 AFV.select :carrierSolution
@22:47:35.32 delay 2 seconds
@22:47:37.35 AS.reconfigure ASconfig
@22:47:37.41 AS.seek 1ml
@22:48:35.20 delay 1 second
@22:48:36.22 AFV.select :holdingCoil
@22:48:36.32 delay 1 second
@22:48:37.34 AS.reconfigure ASfast
@22:48:37.40 AS.seek 0ml
@22:49:04.75 delay 3 seconds
@22:49:07.77 AFV.select :carrierSolution
@22:49:07.87 delay 2 seconds
@22:49:09.89 AS.reconfigure ASconfig
@22:49:09.96 AS.seek 1ml
@22:50:07.70 delay 1 second
@22:50:08.72 AFV.select :holdingCoil
@22:50:08.82 delay 1 second
@22:50:09.84 AS.reconfigure ASnorm
@22:50:09.90 AS.seek 0ml
@22:51:00.60 Setting up PCR for ACOPP5 -- writing /var/log/esp/ACOPP521apr2722h.pcr
@22:51:00.62 Building PCR Slug from ASV with MM3 mix, PP5 primer
@22:51:00.64 Priming with carrier
@22:51:00.66 ATV.dial ARV
@22:51:01.33 ARV.dial :carrier
@22:51:01.85 AS.seek 0.5ml
@22:51:27.50 AFV.select :carrierSolution
@22:51:27.61 delay 2 seconds
@22:51:29.65 AS.reconfigure ASconfig
@22:51:29.71 AS.seek 1ml
@22:51:58.73 delay 1 second
@22:51:59.75 AFV.select :holdingCoil
@22:51:59.85 delay 1 second
@22:52:00.87 ATV.dial :waste
@22:52:02.65 AS.reconfigure ASnorm
@22:52:02.71 AS.seek 0ml
@22:52:53.40 AFV.select :carrierSolution
@22:52:53.52 delay 2 seconds
@22:52:55.55 AS.reconfigure ASconfig
@22:52:55.60 AS.seek 0.5ml
@22:53:24.68 delay 1 second
@22:53:25.70 AFV.select :holdingCoil
@22:53:25.80 Building Slug
@22:53:25.82 ATV.dial :ARV
@22:53:27.56 delay 2 seconds
@22:53:29.58 ARV.dial :air1
@22:53:30.01 delay 2 seconds
@22:53:32.48 AS.reconfigure ASslow2
@22:53:32.54 AS.seek 0.506ml
@22:53:38.89 delay 2 seconds
@22:53:40.91 ARV.dial :MM3
@22:53:42.03 delay 2 seconds
@22:53:44.05 Adding MM3
@22:53:44.14 AS.seek 0.524ml
@22:54:02.64 delay 25 seconds
@22:54:27.66 ARV.dial :PP5
@22:54:28.08 Adding PP5
@22:54:28.09 delay 2 seconds
@22:54:30.17 AS.seek 0.53ml
@22:54:36.90 delay 10 seconds
@22:54:46.93 ARV.dial :air3
@22:54:47.36 adding ARV air
@22:54:47.37 delay 2 seconds
@22:54:49.45 AS.seek 0.536ml
@22:54:55.87 delay 5 seconds
@22:55:00.89 ARV.dial :carrier
@22:55:02.23 delay 7 seconds
@22:55:09.31 AS.seek 0.594ml
@22:56:08.01 delay 5 seconds
@22:56:13.03 ATV.dial :ASV
@22:56:13.47 delay 2 seconds
@22:56:15.54 AS.seek 0.6ml
@22:56:21.81 delay 4 seconds
@22:56:25.83 ATV.dial ARV
@22:56:26.44 delay 2 seconds
@22:56:28.47 ARV.dial :carrier
@22:56:28.48 delay 2 seconds
@22:56:30.57 AS.seek 0.65ml
@22:57:21.17 delay 4 seconds
@22:57:25.19 ATV.dial PCR
@22:57:25.83 ASV.dial :waste1
@22:57:25.84 ARV.dial :air1
@22:57:26.27 Positioning Slug by 0.324ml
@22:57:26.36 AS.seek 0.326ml
@23:02:52.70 delay 5 seconds
@23:02:57.72 ATV.dial ARV
@23:02:58.54 ASV.dial :PCRlock
@23:02:58.95 delay 5 seconds
@23:03:03.97 Running ACOPP5 ProfileIPc thermocycling
@23:03:03.99 <PCR> PCR.program ProfileIPc
@23:03:04.10 ProfileIPc should complete in < 1:26:35
@23:03:04.11 PCR.run 1:53:13.75
@00:11:25.61CEST28-Apr-21 PCR.off
@00:11:26.18 <MFBPCR> PCR for ACOPP5 completed -- closed /var/log/esp/ACOPP521apr2722h.pcr
@00:11:26.25 Cleaning PCR
@00:11:26.26 AFV.select :carrierSolution
@00:11:26.38 delay 2 seconds
@00:11:28.41 AS.reconfigure ASconfig
@00:11:28.46 AS.seek 0.5ml
@00:11:38.75 delay 1 second
@00:11:39.77 AFV.select :holdingCoil
@00:11:39.87 ASV.dial :waste1
@00:11:40.28 ATV.dial :air3
@00:11:41.64 AS.reconfigure ASslow1
@00:11:41.70 AS.seek 0.51ml
@00:11:44.10 delay 2 seconds
@00:11:46.12 ATV.dial :bleach
@00:11:46.75 AS.seek 0.81ml
@00:12:47.48 delay 3 seconds
@00:12:50.50 ATV.dial :air3
@00:12:51.09 delay 3 seconds
@00:12:54.16 AS.seek 0.835ml
@00:12:59.48 delay 3 seconds
@00:13:02.50 ATV.dial :bleach
@00:13:03.15 AS.seek 0.82ml
@00:13:06.51 delay 3 seconds
@00:13:09.53 ARV.dial :waste
@00:13:10.17 delay 3 seconds
@00:13:13.19 ATV.dial :ARV
@00:13:14.13 AS.reconfigure ASconfig
@00:13:14.19 AS.seek 0.47ml
@00:13:34.62 AFV.select :carrierSolution
@00:13:34.73 delay 2 seconds
@00:13:36.76 AS.seek 1ml
@00:14:07.50 delay 1 second
@00:14:08.52 AFV.select :holdingCoil
@00:14:08.62 delay 1 second
@00:14:09.65 AS.reconfigure ASfast
@00:14:09.70 AS.seek 0ml
@00:14:37.05 AFV.select :carrierSolution
@00:14:37.16 delay 2 seconds
@00:14:39.19 AS.reconfigure ASconfig
@00:14:39.24 AS.seek 1ml
@00:15:36.98 delay 1 second
@00:15:38.00 AFV.select :holdingCoil
@00:15:38.11 delay 1 second
@00:15:39.14 AS.reconfigure ASfast
@00:15:39.19 AS.seek 0ml
@00:16:06.53 AFV.select :carrierSolution
@00:16:06.64 delay 2 seconds
@00:16:08.67 AS.reconfigure ASconfig
@00:16:08.72 AS.seek 1ml
@00:17:06.51 delay 1 second
@00:17:07.53 AFV.select :holdingCoil
@00:17:07.63 delay 1 second
@00:17:08.65 AS.reconfigure ASfast
@00:17:08.71 AS.seek 0ml
@00:17:36.05 ARV.dial between carrier and air1
@00:17:36.58 ATV.dial :air3
@00:17:37.93 AS.reconfigure ASslow1
@00:17:37.99 AS.seek 0.01ml
@00:17:40.34 delay 2 seconds
@00:17:42.36 ATV.dial :bleach
@00:17:43.01 AS.seek 0.81ml
@00:20:24.14 delay 3 seconds
@00:20:27.16 ATV.dial :air3
@00:20:27.75 delay 3 seconds
@00:20:30.82 AS.seek 0.835ml
@00:20:36.12 delay 3 seconds
@00:20:39.14 ATV.dial :bleach
@00:20:39.78 AS.seek 0.82ml
@00:20:43.09 delay 3 seconds
@00:20:46.11 ATV.dial PCR
@00:20:46.53 AS.reconfigure ASconfig
@00:20:46.59 AS.seek 0ml
@00:21:34.00 PCR.program :bleachProfile
@00:21:34.06 PCR program should complete in < 2:45
@00:21:34.07 PCR.run 10:00
@00:24:30.30 PCR.off
@00:24:30.35 AFV.select :carrierSolution
@00:24:30.46 delay 2 seconds
@00:24:32.49 AS.seek 1ml
@00:25:30.28 delay 1 second
@00:25:31.30 AFV.select :holdingCoil
@00:25:31.40 delay 1 second
@00:25:32.43 AS.reconfigure ASfast
@00:25:32.48 AS.seek 0ml
@00:25:59.83 delay 3 seconds
@00:26:02.85 AFV.select :carrierSolution
@00:26:02.95 delay 2 seconds
@00:26:04.97 AS.reconfigure ASconfig
@00:26:05.04 AS.seek 1ml
@00:27:02.81 delay 1 second
@00:27:03.83 AFV.select :holdingCoil
@00:27:03.93 delay 1 second
@00:27:04.95 AS.reconfigure ASfast
@00:27:05.01 AS.seek 0ml
@00:27:32.34 delay 3 seconds
@00:27:35.36 AFV.select :carrierSolution
@00:27:35.46 delay 2 seconds
@00:27:37.50 AS.reconfigure ASconfig
@00:27:37.56 AS.seek 1ml
@00:28:35.36 delay 1 second
@00:28:36.38 AFV.select :holdingCoil
@00:28:36.48 delay 1 second
@00:28:37.50 AS.reconfigure ASfast
@00:28:37.56 AS.seek 0ml
@00:29:04.89 delay 3 seconds
@00:29:07.91 AFV.select :carrierSolution
@00:29:08.03 delay 2 seconds
@00:29:10.05 AS.reconfigure ASconfig
@00:29:10.12 AS.seek 1ml
@00:30:07.87 delay 1 second
@00:30:08.89 AFV.select :holdingCoil
@00:30:08.99 delay 1 second
@00:30:10.02 AS.reconfigure ASfast
@00:30:10.07 AS.seek 0ml
@00:30:37.41 delay 3 seconds
@00:30:40.43 AFV.select :carrierSolution
@00:30:40.53 delay 2 seconds
@00:30:42.55 AS.reconfigure ASconfig
@00:30:42.61 AS.seek 1ml
@00:31:40.36 delay 1 second
@00:31:41.38 AFV.select :holdingCoil
@00:31:41.48 delay 1 second
@00:31:42.51 AS.reconfigure ASnorm
@00:31:42.56 AS.seek 0ml
@00:32:33.27 Setting up PCR for AIPc -- writing /var/log/esp/AIPc21apr2800h.pcr
@00:32:33.29 Building PCR Slug from ASV with MM3 mix, PP6 primer
@00:32:33.31 Priming with carrier
@00:32:33.33 ATV.dial ARV
@00:32:33.98 ARV.dial :carrier
@00:32:34.32 AS.seek 0.5ml
@00:32:59.86 AFV.select :carrierSolution
@00:32:59.98 delay 2 seconds
@00:33:02.01 AS.reconfigure ASconfig
@00:33:02.06 AS.seek 1ml
@00:33:31.09 delay 1 second
@00:33:32.11 AFV.select :holdingCoil
@00:33:32.21 delay 1 second
@00:33:33.23 ATV.dial :waste
@00:33:35.00 AS.reconfigure ASnorm
@00:33:35.06 AS.seek 0ml
@00:34:25.78 AFV.select :carrierSolution
@00:34:25.90 delay 2 seconds
@00:34:27.93 AS.reconfigure ASconfig
@00:34:27.98 AS.seek 0.5ml
@00:34:57.01 delay 1 second
@00:34:58.03 AFV.select :holdingCoil
@00:34:58.54 Building Slug
@00:34:58.55 ATV.dial :ARV
@00:35:00.30 delay 2 seconds
@00:35:02.32 ARV.dial :air1
@00:35:02.73 delay 2 seconds
@00:35:04.80 AS.reconfigure ASslow2
@00:35:04.86 AS.seek 0.506ml
@00:35:11.23 delay 2 seconds
@00:35:13.25 ARV.dial :MM3
@00:35:14.37 delay 2 seconds
@00:35:16.39 Adding MM3
@00:35:16.48 AS.seek 0.524ml
@00:35:34.83 delay 25 seconds
@00:35:59.85 ARV.dial :PP6
@00:36:00.26 Adding PP6
@00:36:00.27 delay 2 seconds
@00:36:02.35 AS.seek 0.53ml
@00:36:08.50 delay 10 seconds
@00:36:18.53 ARV.dial :air1
@00:36:19.41 adding ARV air
@00:36:19.42 delay 2 seconds
@00:36:21.50 AS.seek 0.536ml
@00:36:27.95 delay 5 seconds
@00:36:32.97 ARV.dial :carrier
@00:36:33.41 delay 7 seconds
@00:36:40.49 AS.seek 0.594ml
@00:37:38.98 delay 5 seconds
@00:37:44.00 ATV.dial :ASV
@00:37:44.42 delay 2 seconds
@00:37:46.49 AS.seek 0.6ml
@00:37:53.14 delay 4 seconds
@00:37:57.16 ATV.dial ARV
@00:37:57.75 delay 2 seconds
@00:37:59.77 ARV.dial :carrier
@00:37:59.78 delay 2 seconds
@00:38:01.86 AS.seek 0.65ml
@00:38:52.72 delay 4 seconds
@00:38:56.74 ATV.dial PCR
@00:38:57.37 ASV.dial :waste1
@00:38:57.38 ARV.dial :air1
@00:38:57.81 Positioning Slug by 0.324ml
@00:38:57.90 AS.seek 0.326ml
@00:44:24.05 delay 5 seconds
@00:44:29.07 ATV.dial ARV
@00:44:29.89 ASV.dial :PCRlock
@00:44:30.30 delay 5 seconds
@00:44:35.32 Running AIPc ProfileIPc thermocycling
@00:44:35.34 <PCR> PCR.program ProfileIPc
@00:44:35.46 ProfileIPc should complete in < 1:26:35
@00:44:35.47 PCR.run 1:53:13.75
@01:53:01.06 PCR.off
@01:53:01.63 <MFBPCR> PCR for AIPc completed -- closed /var/log/esp/AIPc21apr2800h.pcr
@01:53:01.64 Full MFB Decontamination
@01:53:01.66 Decon extraction column and decon extSrc
@01:53:01.69 SPE.hold 2:46:40,55C
@01:53:01.74 ASV.dial :SPEdelivery
@01:53:02.42 delay 20 seconds
@01:53:22.45 AFV.select :carrierSolution
@01:53:22.55 delay 2 seconds
@01:53:24.58 AS.reconfigure ASconfig
@01:53:24.63 AS.seek 0.5ml
@01:53:34.97 delay 1 second
@01:53:35.99 AFV.select :holdingCoil
@01:53:36.09 ATV.dial :air1
@01:53:38.23 AS.reconfigure ASslow1
@01:53:38.29 AS.seek 0.52ml
@01:53:42.61 ATV.dial :ASV
@01:53:44.56 ASV.dial :SPErecovery
@01:53:45.24 AS.seek 0.62ml
@01:54:05.63 ATV.dial :waste
@01:54:07.84 AS.reconfigure ASnorm
@01:54:07.90 AS.seek 0ml
@01:54:39.49 deconEluateRecoveryLine extSrc
@01:54:39.50 ATV.dial :waste
@01:54:39.53 AS.seek 0ml
@01:54:39.80 ATV.dial :air3
@01:54:40.58 AS.reconfigure ASslow1
@01:54:40.64 AS.seek 0.01ml
@01:54:43.06 delay 2 seconds
@01:54:45.08 ATV.dial :bleach
@01:54:45.59 AS.seek 0.31ml
@01:55:46.30 delay 3 seconds
@01:55:49.32 ATV.dial :air3
@01:55:49.95 delay 3 seconds
@01:55:53.02 AS.seek 0.335ml
@01:55:58.28 delay 3 seconds
@01:56:01.30 ATV.dial :bleach
@01:56:01.98 AS.seek 0.32ml
@01:56:05.28 delay 3 seconds
@01:56:08.30 ATV.dial :extSrc
@01:56:08.99 AS.seek 0ml
@01:57:13.80 AFV.select :carrierSolution
@01:57:13.91 delay 2 seconds
@01:57:15.94 AS.reconfigure ASconfig
@01:57:15.99 AS.seek 0.5ml
@01:57:45.02 delay 1 second
@01:57:46.04 AFV.select :holdingCoil
@01:57:46.14 ATV.dial :extSrc
@01:57:46.16 AS.reconfigure ASslow1
@01:57:46.22 AS.seek 0ml
@01:59:27.20 AFV.select :carrierSolution
@01:59:27.31 delay 2 seconds
@01:59:29.34 AS.reconfigure ASconfig
@01:59:29.39 AS.seek 0.5ml
@01:59:58.42 delay 1 second
@01:59:59.44 AFV.select :holdingCoil
@01:59:59.54 ATV.dial :extSrc
@01:59:59.56 AS.reconfigure ASslow1
@01:59:59.62 AS.seek 0ml
@02:01:40.50 AFV.select :carrierSolution
@02:01:40.61 delay 2 seconds
@02:01:42.64 AS.reconfigure ASconfig
@02:01:42.69 AS.seek 0.5ml
@02:02:11.72 delay 1 second
@02:02:12.74 AFV.select :holdingCoil
@02:02:12.84 ATV.dial :extSrc
@02:02:12.86 AS.reconfigure ASslow1
@02:02:12.92 AS.seek 0ml
@02:03:53.77 AFV.select :carrierSolution
@02:03:53.88 delay 2 seconds
@02:03:55.91 AS.reconfigure ASconfig
@02:03:56.38 AS.seek 0.5ml
@02:04:25.42 delay 1 second
@02:04:26.44 AFV.select :holdingCoil
@02:04:26.54 ATV.dial :extSrc
@02:04:26.56 AS.reconfigure ASslow1
@02:04:26.63 AS.seek 0ml
@02:06:07.45 ATV.dial :air2
@02:06:08.84 AS.reconfigure ASslow0
@02:06:08.90 AS.seek 0.5ml
@02:06:59.53 ATV.dial :extSrc
@02:07:01.09 AS.seek 0.02ml
@02:07:49.70 delay 1 second
@02:07:50.72 ATV.dial :waste
@02:07:52.09 AS.reconfigure ASconfig
@02:07:52.15 AS.seek 0ml
@02:07:53.61 AFV.select :holdingCoil
@02:07:53.62 ATV.dial :waste
@02:07:53.65 AS.seek 0ml
@02:07:53.73 AFV.select :carrierSolution
@02:07:53.85 delay 2 seconds
@02:07:55.88 AS.seek 0.5ml
@02:08:24.94 delay 1 second
@02:08:25.96 AFV.select :holdingCoil
@02:08:26.06 ATV.dial :air3
@02:08:26.93 AS.reconfigure ASslow1
@02:08:26.99 AS.seek 0.52ml
@02:08:31.20 ATV.dial :air3
@02:08:31.29 AS.seek 0.53ml
@02:08:33.59 delay 2 seconds
@02:08:35.61 ATV.dial :bleach
@02:08:36.11 AS.seek 0.71ml
@02:09:12.58 delay 3 seconds
@02:09:15.60 ATV.dial :air3
@02:09:16.19 delay 3 seconds
@02:09:19.26 AS.seek 0.735ml
@02:09:24.67 delay 3 seconds
@02:09:27.69 ATV.dial :bleach
@02:09:28.34 AS.seek 0.72ml
@02:09:31.64 delay 3 seconds
@02:09:34.66 ATV.dial ASV
@02:09:35.77 ASV.dial :SPErecovery
@02:09:35.86 AS.seek 0.365ml
@02:10:47.52 ATV.dial :waste
@02:10:49.53 AS.reconfigure ASnorm
@02:10:49.59 AS.seek 0ml
@02:11:08.33 ATV.dial :air3
@02:11:09.22 AS.reconfigure ASslow1
@02:11:09.28 AS.seek 0.01ml
@02:11:11.77 delay 2 seconds
@02:11:13.79 ATV.dial :bleach
@02:11:14.28 AS.seek 0.58ml
@02:13:09.20 delay 3 seconds
@02:13:12.22 ATV.dial :air3
@02:13:12.81 delay 3 seconds
@02:13:15.88 AS.seek 0.605ml
@02:13:21.18 delay 3 seconds
@02:13:24.20 ATV.dial :bleach
@02:13:24.84 AS.seek 0.59ml
@02:13:28.08 delay 3 seconds
@02:13:31.10 ATV.dial :ASV
@02:13:32.22 ASV.dial :wasteR
@02:13:32.63 AS.seek 0ml
@02:15:31.59 delay 1:00
@02:16:31.61 ATV.dial ASV
@02:16:31.62 AFV.select :carrierSolution
@02:16:31.74 delay 2 seconds
@02:16:33.76 AS.reconfigure ASconfig
@02:16:33.82 AS.seek 1ml
@02:17:31.58 delay 1 second
@02:17:32.60 AFV.select :holdingCoil
@02:17:32.70 delay 1 second
@02:17:33.72 ASV.dial :wasteR
@02:17:33.74 AS.reconfigure ASslow1
@02:17:33.81 AS.seek 0ml
@02:20:55.19 delay 10 seconds
@02:21:05.22 ATV.dial :air3
@02:21:06.83 AS.seek 0.01ml
@02:21:09.25 delay 2 seconds
@02:21:11.27 ATV.dial :bleach
@02:21:11.92 AS.seek 0.58ml
@02:23:06.80 delay 3 seconds
@02:23:09.82 ATV.dial :air3
@02:23:10.41 delay 3 seconds
@02:23:13.48 AS.seek 0.605ml
@02:23:18.86 delay 3 seconds
@02:23:21.88 ATV.dial :bleach
@02:23:22.53 AS.seek 0.59ml
@02:23:25.81 delay 3 seconds
@02:23:28.83 ATV.dial ASV
@02:23:29.94 ASV.dial :SPEdelivery
@02:23:30.33 AS.seek 0ml
@02:25:29.42 delay 1:00
@02:26:29.44 AFV.select :carrierSolution
@02:26:29.55 delay 2 seconds
@02:26:31.58 AS.reconfigure ASconfig
@02:26:31.63 AS.seek 1ml
@02:27:29.39 delay 1 second
@02:27:30.41 AFV.select :holdingCoil
@02:27:30.51 delay 1 second
@02:27:31.53 ATV.dial ASV
@02:27:31.54 ASV.dial :SPEdelivery
@02:27:31.56 AS.reconfigure ASslow1
@02:27:31.63 AS.seek 0ml
@02:30:53.05 delay 10 seconds
@02:31:03.08 ATV.dial :air2
@02:31:03.97 AS.reconfigure ASslow0
@02:31:04.03 AS.seek 0.8ml
@02:32:25.27 delay 3 seconds
@02:32:28.29 ATV.dial :ASV
@02:32:29.35 ASV.dial :SPEdelivery
@02:32:29.37 AS.reconfigure ASslow1
@02:32:29.43 AS.seek 0.03ml
@02:35:04.63 delay 20 seconds
@02:35:24.66 ATV.dial :waste,via: :air3
@02:35:26.50 AS.reconfigure ASconfig
@02:35:26.56 AS.seek 0ml
@02:35:28.60 SPE.coast
@02:35:28.68 Removing Bleach from Mixing Coil
@02:35:28.69 AFV.select :carrierSolution
@02:35:28.81 delay 2 seconds
@02:35:30.84 AS.seek 0.5ml
@02:35:59.91 delay 1 second
@02:36:00.93 AFV.select :holdingCoil
@02:36:01.03 ATV.dial :ASV
@02:36:02.99 ASV.dial :SPErecovery
@02:36:03.66 AS.reconfigure ASslow1
@02:36:03.72 AS.seek 0.73ml
@02:36:50.24 ASV.dial :wasteD
@02:36:50.85 AS.reconfigure ASnorm
@02:36:50.91 AS.seek 0ml
@02:37:28.03 AFV.select :carrierSolution
@02:37:28.15 delay 2 seconds
@02:37:30.18 AS.reconfigure ASconfig
@02:37:30.23 AS.seek 0.5ml
@02:37:59.28 delay 1 second
@02:38:00.30 AFV.select :holdingCoil
@02:38:00.40 ATV.dial ASV
@02:38:00.42 ASV.dial :SPErecovery
@02:38:01.11 AS.reconfigure ASslow0
@02:38:01.17 AS.seek 0.3ml
@02:38:21.63 ATV.dial :air2
@02:38:22.56 AS.reconfigure ASslow1
@02:38:22.61 AS.seek 0.32ml
@02:38:26.96 ATV.dial ASV
@02:38:28.06 AS.reconfigure ASslow0
@02:38:28.12 AS.seek 0.57ml
@02:38:53.52 ASV.dial :wasteD
@02:38:54.14 AS.reconfigure ASnorm
@02:38:54.20 AS.seek 0ml
@02:39:23.24 AFV.select :carrierSolution
@02:39:23.35 delay 2 seconds
@02:39:25.38 AS.reconfigure ASconfig
@02:39:25.43 AS.seek 0.5ml
@02:39:54.45 delay 1 second
@02:39:55.47 AFV.select :holdingCoil
@02:39:55.57 ATV.dial ASV
@02:39:55.59 ASV.dial :SPErecovery
@02:39:56.27 AS.reconfigure ASslow0
@02:39:56.33 AS.seek 0.3ml
@02:40:16.80 ATV.dial :air2
@02:40:17.88 AS.reconfigure ASslow1
@02:40:17.94 AS.seek 0.32ml
@02:40:22.35 ATV.dial ASV
@02:40:23.44 AS.reconfigure ASslow0
@02:40:23.50 AS.seek 0.57ml
@02:40:48.99 ASV.dial :wasteD
@02:40:49.61 AS.reconfigure ASnorm
@02:40:49.67 AS.seek 0ml
@02:41:18.74 ATV.dial :air2
@02:41:19.77 AS.reconfigure ASslow0
@02:41:19.83 AS.seek 0.3ml
@02:41:50.31 ATV.dial :ASV
@02:41:51.33 ASV.dial :wasteD
@02:41:51.35 AS.reconfigure ASconfig
@02:41:51.42 AS.seek 0.05ml
@02:42:06.09 ATV.dial :waste
@02:42:08.05 AS.seek 0ml
@02:42:11.25 ASV.dial :SPEdelivery
@02:42:12.35 Cleaning PCR
@02:42:12.36 AFV.select :carrierSolution
@02:42:12.47 delay 2 seconds
@02:42:14.50 AS.seek 0.5ml
@02:42:43.56 delay 1 second
@02:42:44.58 AFV.select :holdingCoil
@02:42:44.68 ASV.dial :waste1
@02:42:45.06 ATV.dial :air3
@02:42:45.79 AS.reconfigure ASslow1
@02:42:45.86 AS.seek 0.51ml
@02:42:48.24 delay 2 seconds
@02:42:50.26 ATV.dial :bleach
@02:42:50.73 AS.seek 0.81ml
@02:43:51.41 delay 3 seconds
@02:43:54.43 ATV.dial :air3
@02:43:55.03 delay 3 seconds
@02:43:58.10 AS.seek 0.835ml
@02:44:03.42 delay 3 seconds
@02:44:06.44 ATV.dial :bleach
@02:44:07.14 AS.seek 0.82ml
@02:44:10.60 delay 3 seconds
@02:44:13.62 ARV.dial :waste
@02:44:14.25 delay 3 seconds
@02:44:17.27 ATV.dial :ARV
@02:44:18.19 AS.reconfigure ASconfig
@02:44:18.26 AS.seek 0.47ml
@02:44:38.66 AFV.select :carrierSolution
@02:44:38.77 delay 2 seconds
@02:44:40.80 AS.seek 1ml
@02:45:11.55 delay 1 second
@02:45:12.57 AFV.select :holdingCoil
@02:45:12.67 delay 1 second
@02:45:13.70 AS.reconfigure ASfast
@02:45:13.75 AS.seek 0ml
@02:45:41.08 AFV.select :carrierSolution
@02:45:41.62 delay 2 seconds
@02:45:43.65 AS.reconfigure ASconfig
@02:45:43.70 AS.seek 1ml
@02:46:41.50 delay 1 second
@02:46:42.52 AFV.select :holdingCoil
@02:46:42.62 delay 1 second
@02:46:43.65 AS.reconfigure ASfast
@02:46:43.70 AS.seek 0ml
@02:47:11.03 AFV.select :carrierSolution
@02:47:11.14 delay 2 seconds
@02:47:13.17 AS.reconfigure ASconfig
@02:47:13.22 AS.seek 1ml
@02:48:10.97 delay 1 second
@02:48:11.99 AFV.select :holdingCoil
@02:48:12.09 delay 1 second
@02:48:13.12 AS.reconfigure ASfast
@02:48:13.17 AS.seek 0ml
@02:48:40.52 ARV.dial between carrier and air1
@02:48:41.05 ATV.dial :air3
@02:48:42.41 AS.reconfigure ASslow1
@02:48:42.47 AS.seek 0.01ml
@02:48:44.89 delay 2 seconds
@02:48:46.91 ATV.dial :bleach
@02:48:47.56 AS.seek 0.81ml
@02:51:28.81 delay 3 seconds
@02:51:31.83 ATV.dial :air3
@02:51:32.42 delay 3 seconds
@02:51:35.49 AS.seek 0.835ml
@02:51:40.83 delay 3 seconds
@02:51:43.85 ATV.dial :bleach
@02:51:44.50 AS.seek 0.82ml
@02:51:47.81 delay 3 seconds
@02:51:50.83 ATV.dial PCR
@02:51:51.25 AS.reconfigure ASconfig
@02:51:51.31 AS.seek 0ml
@02:52:38.72 PCR.program :bleachProfile
@02:52:38.79 PCR program should complete in < 2:45
@02:52:38.80 PCR.run 10:00
@02:55:40.64 PCR.off
@02:55:40.68 AFV.select :carrierSolution
@02:55:40.79 delay 2 seconds
@02:55:42.82 AS.seek 1ml
@02:56:40.58 delay 1 second
@02:56:41.60 AFV.select :holdingCoil
@02:56:41.70 delay 1 second
@02:56:42.72 AS.reconfigure ASfast
@02:56:42.78 AS.seek 0ml
@02:57:10.11 delay 3 seconds
@02:57:13.13 AFV.select :carrierSolution
@02:57:13.23 delay 2 seconds
@02:57:15.25 AS.reconfigure ASconfig
@02:57:15.32 AS.seek 1ml
@02:58:13.06 delay 1 second
@02:58:14.08 AFV.select :holdingCoil
@02:58:14.18 delay 1 second
@02:58:15.21 AS.reconfigure ASfast
@02:58:15.26 AS.seek 0ml
@02:58:42.61 delay 3 seconds
@02:58:45.63 AFV.select :carrierSolution
@02:58:46.15 delay 2 seconds
@02:58:48.18 AS.reconfigure ASconfig
@02:58:48.23 AS.seek 1ml
@02:59:45.97 delay 1 second
@02:59:46.99 AFV.select :holdingCoil
@02:59:47.09 delay 1 second
@02:59:48.12 AS.reconfigure ASfast
@02:59:48.17 AS.seek 0ml
@03:00:15.50 delay 3 seconds
@03:00:18.52 AFV.select :carrierSolution
@03:00:18.62 delay 2 seconds
@03:00:20.65 AS.reconfigure ASconfig
@03:00:20.71 AS.seek 1ml
@03:01:18.50 delay 1 second
@03:01:19.52 AFV.select :holdingCoil
@03:01:19.62 delay 1 second
@03:01:20.65 AS.reconfigure ASfast
@03:01:20.70 AS.seek 0ml
@03:01:48.05 delay 3 seconds
@03:01:51.07 AFV.select :carrierSolution
@03:01:51.17 delay 2 seconds
@03:01:53.19 AS.reconfigure ASconfig
@03:01:53.26 AS.seek 1ml
@03:02:51.05 delay 1 second
@03:02:52.07 AFV.select :holdingCoil
@03:02:52.17 delay 1 second
@03:02:53.20 AS.reconfigure ASnorm
@03:02:53.25 AS.seek 0ml
@03:03:43.96 AFV.select :carrierSolution
@03:03:44.06 delay 2 seconds
@03:03:46.09 AS.reconfigure ASconfig
@03:03:46.14 AS.seek 1ml
@03:04:43.89 delay 1 second
@03:04:44.91 AFV.select :holdingCoil
@03:04:45.01 delay 1 second
@03:04:46.03 ATV.dial :waste
@03:04:47.33 AS.reconfigure ASnorm
@03:04:47.39 AS.seek 0.5ml
@03:05:12.97 ATV.dial :waste2
@03:05:14.31 AS.seek 0ml
@03:05:39.88 <MAIN> Cleaning PCR
@03:05:39.89 AFV.select :carrierSolution
@03:05:40.01 delay 2 seconds
@03:05:42.04 AS.reconfigure ASconfig
@03:05:42.09 AS.seek 0.5ml
@03:06:11.16 delay 1 second
@03:06:12.18 AFV.select :holdingCoil
@03:06:12.28 ASV.dial :waste1
@03:06:12.29 ATV.dial :air3
@03:06:14.35 AS.reconfigure ASslow1
@03:06:14.41 AS.seek 0.51ml
@03:06:16.67 delay 2 seconds
@03:06:18.69 ATV.dial :bleach
@03:06:19.17 AS.seek 0.81ml
@03:07:19.75 delay 3 seconds
@03:07:22.77 ATV.dial :air3
@03:07:23.36 delay 3 seconds
@03:07:26.43 AS.seek 0.835ml
@03:07:31.81 delay 3 seconds
@03:07:34.83 ATV.dial :bleach
@03:07:35.48 AS.seek 0.82ml
@03:07:38.77 delay 3 seconds
@03:07:41.79 ARV.dial :waste
@03:07:42.31 delay 3 seconds
@03:07:45.33 ATV.dial :ARV
@03:07:46.25 AS.reconfigure ASconfig
@03:07:46.31 AS.seek 0.47ml
@03:08:06.72 AFV.select :carrierSolution
@03:08:06.83 delay 2 seconds
@03:08:08.86 AS.seek 1ml
@03:08:39.66 delay 1 second
@03:08:40.68 AFV.select :holdingCoil
@03:08:40.78 delay 1 second
@03:08:41.81 AS.reconfigure ASfast
@03:08:41.86 AS.seek 0ml
@03:09:09.20 AFV.select :carrierSolution
@03:09:09.31 delay 2 seconds
@03:09:11.33 AS.reconfigure ASconfig
@03:09:11.39 AS.seek 1ml
@03:10:09.13 delay 1 second
@03:10:10.15 AFV.select :holdingCoil
@03:10:10.25 delay 1 second
@03:10:11.27 AS.reconfigure ASfast
@03:10:11.33 AS.seek 0ml
@03:10:38.67 AFV.select :carrierSolution
@03:10:38.78 delay 2 seconds
@03:10:40.80 AS.reconfigure ASconfig
@03:10:40.86 AS.seek 1ml
@03:11:38.64 delay 1 second
@03:11:39.66 AFV.select :holdingCoil
@03:11:39.76 delay 1 second
@03:11:40.78 AS.reconfigure ASfast
@03:11:40.84 AS.seek 0ml
@03:12:08.17 ARV.dial between carrier and air1
@03:12:08.69 ATV.dial :air3
@03:12:10.02 AS.reconfigure ASslow1
@03:12:10.08 AS.seek 0.01ml
@03:12:12.50 delay 2 seconds
@03:12:14.52 ATV.dial :bleach
@03:12:15.16 AS.seek 0.81ml
@03:14:56.33 delay 3 seconds
@03:14:59.35 ATV.dial :air3
@03:14:59.94 delay 3 seconds
@03:15:03.01 AS.seek 0.835ml
@03:15:08.31 delay 3 seconds
@03:15:11.33 ATV.dial :bleach
@03:15:11.97 AS.seek 0.82ml
@03:15:15.27 delay 3 seconds
@03:15:18.29 ATV.dial PCR
@03:15:18.72 AS.reconfigure ASconfig
@03:15:18.78 AS.seek 0ml
@03:16:06.19 PCR.program :bleachProfile
@03:16:06.25 PCR program should complete in < 2:45
@03:16:06.26 PCR.run 10:00
@03:19:08.18 PCR.off
@03:19:08.22 AFV.select :carrierSolution
@03:19:08.33 delay 2 seconds
@03:19:10.36 AS.seek 1ml
@03:20:08.13 delay 1 second
@03:20:09.15 AFV.select :holdingCoil
@03:20:09.25 delay 1 second
@03:20:10.27 AS.reconfigure ASfast
@03:20:10.33 AS.seek 0ml
@03:20:37.67 delay 3 seconds
@03:20:40.69 AFV.select :carrierSolution
@03:20:40.79 delay 2 seconds
@03:20:42.81 AS.reconfigure ASconfig
@03:20:42.88 AS.seek 1ml
@03:21:40.67 delay 1 second
@03:21:41.69 AFV.select :holdingCoil
@03:21:41.79 delay 1 second
@03:21:42.81 AS.reconfigure ASfast
@03:21:42.87 AS.seek 0ml
@03:22:10.22 delay 3 seconds
@03:22:13.24 AFV.select :carrierSolution
@03:22:13.34 delay 2 seconds
@03:22:15.36 AS.reconfigure ASconfig
@03:22:15.43 AS.seek 1ml
@03:23:13.17 delay 1 second
@03:23:14.19 AFV.select :holdingCoil
@03:23:14.29 delay 1 second
@03:23:15.31 AS.reconfigure ASfast
@03:23:15.37 AS.seek 0ml
@03:23:42.69 delay 3 seconds
@03:23:45.71 AFV.select :carrierSolution
@03:23:45.81 delay 2 seconds
@03:23:47.83 AS.reconfigure ASconfig
@03:23:47.90 AS.seek 1ml
@03:24:45.64 delay 1 second
@03:24:46.66 AFV.select :holdingCoil
@03:24:46.76 delay 1 second
@03:24:47.79 AS.reconfigure ASfast
@03:24:47.84 AS.seek 0ml
@03:25:15.17 delay 3 seconds
@03:25:18.19 AFV.select :carrierSolution
@03:25:18.29 delay 2 seconds
@03:25:20.31 AS.reconfigure ASconfig
@03:25:20.38 AS.seek 1ml
@03:26:18.14 delay 1 second
@03:26:19.16 AFV.select :holdingCoil
@03:26:19.26 delay 1 second
@03:26:20.28 AS.reconfigure ASnorm
@03:26:20.34 AS.seek 0ml
@03:27:11.04 delay 5 seconds
@03:27:16.11 Cleaning PCR
@03:27:16.12 AFV.select :carrierSolution
@03:27:16.24 delay 2 seconds
@03:27:18.27 AS.reconfigure ASconfig
@03:27:18.32 AS.seek 0.5ml
@03:27:47.39 delay 1 second
@03:27:48.41 AFV.select :holdingCoil
@03:27:48.51 ASV.dial :waste1
@03:27:48.52 ATV.dial :air3
@03:27:49.43 AS.reconfigure ASslow1
@03:27:49.49 AS.seek 0.51ml
@03:27:51.94 delay 2 seconds
@03:27:53.96 ATV.dial :bleach
@03:27:54.62 AS.seek 0.81ml
@03:28:55.33 delay 3 seconds
@03:28:58.35 ATV.dial :air3
@03:28:58.96 delay 3 seconds
@03:29:02.03 AS.seek 0.835ml
@03:29:07.34 delay 3 seconds
@03:29:10.36 ATV.dial :bleach
@03:29:11.02 AS.seek 0.82ml
@03:29:14.44 delay 3 seconds
@03:29:17.46 ARV.dial :waste
@03:29:18.06 delay 3 seconds
@03:29:21.10 ATV.dial :ARV
@03:29:22.02 AS.reconfigure ASconfig
@03:29:22.08 AS.seek 0.47ml
@03:29:42.52 AFV.select :carrierSolution
@03:29:42.63 delay 2 seconds
@03:29:44.66 AS.seek 1ml
@03:30:15.41 delay 1 second
@03:30:16.43 AFV.select :holdingCoil
@03:30:16.53 delay 1 second
@03:30:17.55 AS.reconfigure ASfast
@03:30:17.61 AS.seek 0ml
@03:30:44.95 AFV.select :carrierSolution
@03:30:45.06 delay 2 seconds
@03:30:47.08 AS.reconfigure ASconfig
@03:30:47.49 AS.seek 1ml
@03:31:45.28 delay 1 second
@03:31:46.30 AFV.select :holdingCoil
@03:31:46.40 delay 1 second
@03:31:47.43 AS.reconfigure ASfast
@03:31:47.48 AS.seek 0ml
@03:32:14.83 AFV.select :carrierSolution
@03:32:14.94 delay 2 seconds
@03:32:16.96 AS.reconfigure ASconfig
@03:32:17.02 AS.seek 1ml
@03:33:14.80 delay 1 second
@03:33:15.82 AFV.select :holdingCoil
@03:33:15.92 delay 1 second
@03:33:16.95 AS.reconfigure ASfast
@03:33:17.00 AS.seek 0ml
@03:33:44.35 ARV.dial between carrier and air1
@03:33:44.88 ATV.dial :air3
@03:33:46.23 AS.reconfigure ASslow1
@03:33:46.28 AS.seek 0.01ml
@03:33:48.75 delay 2 seconds
@03:33:50.77 ATV.dial :bleach
@03:33:51.42 AS.seek 0.81ml
@03:36:32.75 delay 3 seconds
@03:36:35.77 ATV.dial :air3
@03:36:36.36 delay 3 seconds
@03:36:39.43 AS.seek 0.835ml
@03:36:44.74 delay 3 seconds
@03:36:47.76 ATV.dial :bleach
@03:36:48.41 AS.seek 0.82ml
@03:36:51.71 delay 3 seconds
@03:36:54.73 ATV.dial PCR
@03:36:55.14 AS.reconfigure ASconfig
@03:36:55.20 AS.seek 0ml
@03:37:42.63 PCR.program :bleachProfile
@03:37:42.69 PCR program should complete in < 2:45
@03:37:42.70 PCR.run 10:00
@03:40:44.58 PCR.off
@03:40:44.62 AFV.select :carrierSolution
@03:40:44.73 delay 2 seconds
@03:40:46.76 AS.seek 1ml
@03:41:44.52 delay 1 second
@03:41:45.54 AFV.select :holdingCoil
@03:41:45.64 delay 1 second
@03:41:46.66 AS.reconfigure ASfast
@03:41:46.72 AS.seek 0ml
@03:42:14.08 delay 3 seconds
@03:42:17.10 AFV.select :carrierSolution
@03:42:17.20 delay 2 seconds
@03:42:19.22 AS.reconfigure ASconfig
@03:42:19.29 AS.seek 1ml
@03:43:17.08 delay 1 second
@03:43:18.10 AFV.select :holdingCoil
@03:43:18.20 delay 1 second
@03:43:19.22 AS.reconfigure ASfast
@03:43:19.28 AS.seek 0ml
@03:43:46.61 delay 3 seconds
@03:43:49.63 AFV.select :carrierSolution
@03:43:49.73 delay 2 seconds
@03:43:51.75 AS.reconfigure ASconfig
@03:43:51.82 AS.seek 1ml
@03:44:49.58 delay 1 second
@03:44:50.60 AFV.select :holdingCoil
@03:44:50.70 delay 1 second
@03:44:51.72 AS.reconfigure ASfast
@03:44:51.78 AS.seek 0ml
@03:45:19.13 delay 3 seconds
@03:45:22.15 AFV.select :carrierSolution
@03:45:22.61 delay 2 seconds
@03:45:24.63 AS.reconfigure ASconfig
@03:45:24.69 AS.seek 1ml
@03:46:22.44 delay 1 second
@03:46:23.46 AFV.select :holdingCoil
@03:46:23.56 delay 1 second
@03:46:24.59 AS.reconfigure ASfast
@03:46:24.64 AS.seek 0ml
@03:46:51.99 delay 3 seconds
@03:46:55.01 AFV.select :carrierSolution
@03:46:55.11 delay 2 seconds
@03:46:57.13 AS.reconfigure ASconfig
@03:46:57.20 AS.seek 1ml
@03:47:54.94 delay 1 second
@03:47:55.96 AFV.select :holdingCoil
@03:47:56.06 delay 1 second
@03:47:57.08 AS.reconfigure ASnorm
@03:47:57.14 AS.seek 0ml
@03:48:47.87 delay 5 seconds
@03:48:52.95 Cleaning PCR
AFV.select :carrierSolution
@03:48:53.07 delay 2 seconds
@03:48:55.10 AS.reconfigure ASconfig
@03:48:55.15 AS.seek 0.5ml
@03:49:24.22 delay 1 second
@03:49:25.24 AFV.select :holdingCoil
@03:49:25.34 ASV.dial :waste1
@03:49:25.35 ATV.dial :air3
@03:49:26.22 AS.reconfigure ASslow1
@03:49:26.28 AS.seek 0.51ml
@03:49:28.66 delay 2 seconds
@03:49:30.68 ATV.dial :bleach
@03:49:31.31 AS.seek 0.81ml
@03:50:32.02 delay 3 seconds
@03:50:35.04 ATV.dial :air3
@03:50:35.63 delay 3 seconds
@03:50:38.70 AS.seek 0.835ml
@03:50:44.01 delay 3 seconds
@03:50:47.03 ATV.dial :bleach
@03:50:47.66 AS.seek 0.82ml
@03:50:50.97 delay 3 seconds
@03:50:53.99 ARV.dial :waste
@03:50:54.50 delay 3 seconds
@03:50:57.52 ATV.dial :ARV
@03:50:58.44 AS.reconfigure ASconfig
@03:50:58.50 AS.seek 0.47ml
@03:51:18.91 AFV.select :carrierSolution
@03:51:19.02 delay 2 seconds
@03:51:21.05 AS.seek 1ml
@03:51:51.83 delay 1 second
@03:51:52.85 AFV.select :holdingCoil
@03:51:52.95 delay 1 second
@03:51:53.98 AS.reconfigure ASfast
@03:51:54.03 AS.seek 0ml
@03:52:21.38 AFV.select :carrierSolution
@03:52:21.49 delay 2 seconds
@03:52:23.51 AS.reconfigure ASconfig
@03:52:23.57 AS.seek 1ml
@03:53:21.38 delay 1 second
@03:53:22.40 AFV.select :holdingCoil
@03:53:22.50 delay 1 second
@03:53:23.52 AS.reconfigure ASfast
@03:53:23.58 AS.seek 0ml
@03:53:50.92 AFV.select :carrierSolution
@03:53:51.03 delay 2 seconds
@03:53:53.05 AS.reconfigure ASconfig
@03:53:53.11 AS.seek 1ml
@03:54:50.85 delay 1 second
@03:54:51.87 AFV.select :holdingCoil
@03:54:51.97 delay 1 second
@03:54:52.99 AS.reconfigure ASfast
@03:54:53.05 AS.seek 0ml
@03:55:20.38 ARV.dial between carrier and air1
@03:55:20.91 ATV.dial :air3
@03:55:22.26 AS.reconfigure ASslow1
@03:55:22.32 AS.seek 0.01ml
@03:55:24.91 delay 2 seconds
@03:55:26.93 ATV.dial :bleach
@03:55:27.58 AS.seek 0.81ml
@03:58:08.81 delay 3 seconds
@03:58:11.83 ATV.dial :air3
@03:58:12.42 delay 3 seconds
@03:58:15.49 AS.seek 0.835ml
@03:58:20.76 delay 3 seconds
@03:58:23.78 ATV.dial :bleach
@03:58:24.42 AS.seek 0.82ml
@03:58:27.85 delay 3 seconds
@03:58:30.87 ATV.dial PCR
@03:58:31.30 AS.reconfigure ASconfig
@03:58:31.36 AS.seek 0ml
@03:59:18.78 PCR.program :bleachProfile
@03:59:18.84 PCR program should complete in < 2:45
@03:59:18.85 PCR.run 10:00
@04:02:21.07 PCR.off
@04:02:21.11 AFV.select :carrierSolution
@04:02:21.22 delay 2 seconds
@04:02:23.25 AS.seek 1ml
@04:03:21.03 delay 1 second
@04:03:22.05 AFV.select :holdingCoil
@04:03:22.15 delay 1 second
@04:03:23.18 AS.reconfigure ASfast
@04:03:23.23 AS.seek 0ml
@04:03:50.55 delay 3 seconds
@04:03:53.57 AFV.select :carrierSolution
@04:03:53.67 delay 2 seconds
@04:03:55.69 AS.reconfigure ASconfig
@04:03:55.76 AS.seek 1ml
@04:04:53.50 delay 1 second
@04:04:54.52 AFV.select :holdingCoil
@04:04:54.62 delay 1 second
@04:04:55.65 AS.reconfigure ASfast
@04:04:55.70 AS.seek 0ml
@04:05:23.03 delay 3 seconds
@04:05:26.05 AFV.select :carrierSolution
@04:05:26.15 delay 2 seconds
@04:05:28.17 AS.reconfigure ASconfig
@04:05:28.23 AS.seek 1ml
@04:06:26.00 delay 1 second
@04:06:27.02 AFV.select :holdingCoil
@04:06:27.12 delay 1 second
@04:06:28.14 AS.reconfigure ASfast
@04:06:28.20 AS.seek 0ml
@04:06:55.53 delay 3 seconds
@04:06:58.55 AFV.select :carrierSolution
@04:06:58.65 delay 2 seconds
@04:07:00.67 AS.reconfigure ASconfig
@04:07:00.74 AS.seek 1ml
@04:07:58.49 delay 1 second
@04:07:59.51 AFV.select :holdingCoil
@04:07:59.61 delay 1 second
@04:08:00.63 AS.reconfigure ASfast
@04:08:00.69 AS.seek 0ml
@04:08:28.03 delay 3 seconds
@04:08:31.05 AFV.select :carrierSolution
@04:08:31.15 delay 2 seconds
@04:08:33.17 AS.reconfigure ASconfig
@04:08:33.23 AS.seek 1ml
@04:09:30.97 delay 1 second
@04:09:31.99 AFV.select :holdingCoil
@04:09:32.09 delay 1 second
@04:09:33.11 AS.reconfigure ASnorm
@04:09:33.17 AS.seek 0ml
@04:10:23.88 delay 5 seconds
@04:10:28.96 <bspe> Session Ended
@13:36:35.87CEST28-Apr-21 <MAIN> ESP2 v3.30 I2C Gateway v3.06 for core bus
@13:36:38.85 
Configuration for mer 7/20/20 brent@mbari.org
/home/esp/esp2/type/shallow/mer/configure.rb
@13:36:38.94 
Configuration for MFB3 by preston@mbari.org
/home/esp/esp2/type/mfb/MFB3/configure.rb
@13:36:43.19 Polling Can every 10:00
@13:36:44.91 CS.configure :default
@13:36:44.95 CS.hold
@13:36:45.01 CS.configure :default
@13:36:45.09 PS.configure :default
@13:36:45.17 SS.configure :default
@13:36:45.20 SS.hold
@13:36:45.25 SS.configure :default
@13:36:45.33 CC.configure :default
@13:36:45.37 CC.hold
@13:36:45.45 CC.configure :default
@13:36:45.53 PC.configure :default
@13:36:45.56 PC.hold
@13:36:45.63 PC.configure :default
@13:36:45.73 Forearm.configure :loose
@13:36:45.76 Forearm.hold
@13:36:45.83 Forearm.configure :loose
@13:36:45.90 Elbow.configure :loose
@13:36:45.94 Elbow.hold
@13:36:46.00 Elbow.configure :loose
@13:36:46.09 SC.configure :loose
@13:36:46.13 SC.hold
@13:36:46.20 SC.configure :loose
@13:36:46.27 SE.configure :default
@13:36:46.31 SE.hold
@13:36:46.37 SE.configure :default
@13:36:46.40 CH.coast
@13:36:46.45 CH.configure :default
@13:36:46.48 PH.coast
@13:36:46.52 PH.configure :default
@13:36:46.62 SPE.coast
@13:36:46.67 SPE.configure SPEconfig
@13:36:46.74 AS.configure ASconfig
@13:36:46.78 AS.hold
@13:36:46.82 AS.configure ASconfig
@13:36:46.87 PCR.off
@13:36:46.90 PCR.pidtemp= 50,0.1,0,300,25,25
@13:36:46.93 PCR.caltemp= 2.5051,0.0372,0
@13:36:46.96 All dwarf controllers running firmware version 3.75
@13:36:47.20 Cleanly exited at 13:22:42CEST28-Apr-21
@13:36:47.23 Loading /home/esp/esp2/mission/stdMER2017.rb mission
@13:36:48.18 <bkha> Session Begun
@13:36:48.36 <MAIN> AFV.select :carrierSolution
@13:36:48.50 delay 2 seconds
@13:36:50.53 AS.seek 0.5ml
@13:37:19.55 delay 1 second
@13:37:20.57 AFV.select :holdingCoil
@13:37:20.67 ATV.dial :extSrc
@13:37:21.13 AS.reconfigure ASslow1
@13:37:21.19 AS.seek 0.53ml
@13:37:27.50 delay 5 seconds
@13:37:32.58 AS.reconfigure ASslow2
@13:37:32.63 AS.seek 0.54ml
@13:37:43.14 delay 20 seconds
@13:38:03.21 AS.reconfigure ASslow1
@13:38:03.27 AS.seek 0.57ml
@13:38:09.66 delay 5 seconds
@13:38:14.73 AS.reconfigure ASslow2
@13:38:14.79 AS.seek 0.58ml
@13:38:25.78 delay 20 seconds
@13:38:45.85 AS.reconfigure ASslow1
@13:38:45.91 AS.seek 0.61ml
@13:38:52.19 delay 5 seconds
@13:38:57.27 AS.reconfigure ASslow2
@13:38:57.32 AS.seek 0.62ml
@13:39:07.59 delay 20 seconds
@13:39:27.66 AS.reconfigure ASslow1
@13:39:27.72 AS.seek 0.65ml
@13:39:34.09 delay 5 seconds
@13:39:39.17 AS.reconfigure ASslow2
@13:39:39.22 AS.seek 0.66ml
@13:39:50.02 delay 20 seconds
@13:40:10.05 AS.reconfigure ASslow1
@13:40:10.10 AS.seek 0.705ml
@13:40:19.42 delay 10 seconds
@13:40:29.44 ATV.dial :air1
@13:40:31.63 AS.seek 0.725ml
@13:40:35.92 delay 3 seconds
@13:40:38.94 ATV.dial :waste
@13:40:39.52 AS.reconfigure ASslow0
@13:40:39.58 AS.seek 0ml
@13:41:52.97 <MFBPCR> Setting up PCR for AARPP4 -- writing /var/log/esp/AARPP421apr2813h.pcr
@13:41:53.00 Building PCR Slug from extSrc with MM2 mix, PP4 primer
@13:41:53.02 Priming with carrier
@13:41:53.04 ATV.dial ARV
@13:41:54.63 ARV.dial :carrier
@13:41:55.00 AS.reconfigure ASnorm
@13:41:55.06 AS.seek 0.5ml
@13:42:20.59 AFV.select :carrierSolution
@13:42:20.71 delay 2 seconds
@13:42:22.74 AS.reconfigure ASconfig
@13:42:22.80 AS.seek 1ml
@13:42:51.81 delay 1 second
@13:42:52.83 AFV.select :holdingCoil
@13:42:52.93 delay 1 second
@13:42:53.95 ATV.dial :waste
@13:42:55.73 AS.reconfigure ASnorm
@13:42:55.79 AS.seek 0ml
@13:43:46.48 AFV.select :carrierSolution
@13:43:46.59 delay 2 seconds
@13:43:48.62 AS.reconfigure ASconfig
@13:43:48.67 AS.seek 0.5ml
@13:44:17.73 delay 1 second
@13:44:18.75 AFV.select :holdingCoil
@13:44:18.85 Building Slug
@13:44:18.87 ATV.dial :ARV
@13:44:20.63 delay 2 seconds
@13:44:22.65 ARV.dial :air1
@13:44:23.07 delay 2 seconds
@13:44:25.14 AS.reconfigure ASslow2
@13:44:25.20 AS.seek 0.506ml
@13:44:31.62 delay 2 seconds
@13:44:33.64 ARV.dial :MM2
@13:44:35.25 delay 2 seconds
@13:44:37.27 Adding MM2
@13:44:37.36 AS.seek 0.524ml
@13:44:55.75 delay 25 seconds
@13:45:20.77 ARV.dial :PP4
@13:45:21.20 Adding PP4
@13:45:21.21 delay 2 seconds
@13:45:23.29 AS.seek 0.53ml
@13:45:29.75 delay 10 seconds
@13:45:39.78 ARV.dial :air3
@13:45:40.20 adding ARV air
@13:45:40.21 delay 2 seconds
@13:45:42.29 AS.seek 0.536ml
@13:45:48.75 delay 5 seconds
@13:45:53.77 ARV.dial :carrier
@13:45:55.11 delay 7 seconds
@13:46:02.18 AS.seek 0.594ml
@13:47:00.95 delay 5 seconds
@13:47:05.97 ATV.dial :extSrc
@13:47:06.58 delay 2 seconds
@13:47:08.66 AS.seek 0.6ml
@13:47:11.97 <bkhaa> Session Begun
@13:47:14.91 <MFBPCR> delay 4 seconds
@13:47:15.78 <bkhaa> -> showlog 100
@13:47:18.93 <MFBPCR> ATV.dial ARV
@13:47:19.55 delay 2 seconds
@13:47:21.57 ARV.dial :carrier
@13:47:21.58 delay 2 seconds
@13:47:23.68 AS.seek 0.65ml
@13:48:14.19 delay 4 seconds
@13:48:18.21 ATV.dial PCR
@13:48:19.05 ASV.dial :waste1
@13:48:19.16 ARV.dial :air1
@13:48:19.59 Positioning Slug by 0.324ml
@13:48:19.69 AS.seek 0.326ml
@13:53:46.33 delay 5 seconds
@13:53:51.35 ATV.dial ARV
@13:53:52.17 ASV.dial :PCRlock
@13:53:52.56 delay 5 seconds
@13:53:57.58 Running AARPP4 Profile58CIQ2 thermocycling
@13:53:57.60 <PCR> PCR.program Profile58CIQ2
@13:53:57.74 Profile58CIQ2 should complete in < 1:36:15
@13:53:57.75 PCR.run 2:05:18.75
@15:14:49.38 PCR.off
@15:14:49.94 <MFBPCR> Running AARPP4 MeltProfile58 thermocycling
@15:14:49.95 <PCR> PCR.program MeltProfile58
@15:14:50.07 MeltProfile58 should complete in < 12:33
@15:14:50.08 PCR.run 20:41.25
@15:22:28.94 PCR.off
@15:22:29.92 <MFBPCR> PCR for AARPP4 completed -- closed /var/log/esp/AARPP421apr2813h.pcr
@15:22:30.01 Cleaning PCR
@15:22:30.02 AFV.select :carrierSolution
@15:22:30.15 delay 2 seconds
@15:22:32.18 AS.reconfigure ASconfig
@15:22:32.24 AS.seek 0.5ml
@15:22:42.56 delay 1 second
@15:22:43.58 AFV.select :holdingCoil
@15:22:43.68 ASV.dial :waste1
@15:22:44.08 ATV.dial :air3
@15:22:45.42 AS.reconfigure ASslow1
@15:22:45.49 AS.seek 0.51ml
@15:22:47.77 delay 2 seconds
@15:22:49.79 ATV.dial :bleach
@15:22:50.44 AS.seek 0.81ml
@15:23:51.03 delay 3 seconds
@15:23:54.05 ATV.dial :air3
@15:23:54.64 delay 3 seconds
@15:23:57.71 AS.seek 0.835ml
@15:24:03.01 delay 3 seconds
@15:24:06.03 ATV.dial :bleach
@15:24:06.67 AS.seek 0.82ml
@15:24:10.00 delay 3 seconds
@15:24:13.02 ARV.dial :waste
@15:24:13.69 delay 3 seconds
@15:24:16.71 ATV.dial :ARV
@15:24:17.66 AS.reconfigure ASconfig
@15:24:17.73 AS.seek 0.47ml
@15:24:38.14 AFV.select :carrierSolution
@15:24:38.26 delay 2 seconds
@15:24:40.29 AS.seek 1ml
@15:25:11.03 delay 1 second
@15:25:12.05 AFV.select :holdingCoil
@15:25:12.15 delay 1 second
@15:25:13.18 AS.reconfigure ASfast
@15:25:13.24 AS.seek 0ml
@15:25:40.59 AFV.select :carrierSolution
@15:25:40.71 delay 2 seconds
@15:25:42.74 AS.reconfigure ASconfig
@15:25:42.80 AS.seek 1ml
@15:26:40.56 delay 1 second
@15:26:41.58 AFV.select :holdingCoil
@15:26:41.68 delay 1 second
@15:26:42.71 AS.reconfigure ASfast
@15:26:42.77 AS.seek 0ml
@15:27:10.12 AFV.select :carrierSolution
@15:27:10.24 delay 2 seconds
@15:27:12.27 AS.reconfigure ASconfig
@15:27:12.33 AS.seek 1ml
@15:28:10.12 delay 1 second
@15:28:11.14 AFV.select :holdingCoil
@15:28:11.60 delay 1 second
@15:28:12.67 AS.reconfigure ASfast
@15:28:12.73 AS.seek 0ml
@15:28:40.08 ARV.dial between carrier and air1
@15:28:40.61 ATV.dial :air3
@15:28:41.98 AS.reconfigure ASslow1
@15:28:42.05 AS.seek 0.01ml
@15:28:44.36 delay 2 seconds
@15:28:46.38 ATV.dial :bleach
@15:28:47.06 AS.seek 0.81ml
@15:31:28.31 delay 3 seconds
@15:31:31.33 ATV.dial :air3
@15:31:31.94 delay 3 seconds
@15:31:35.01 AS.seek 0.835ml
@15:31:40.31 delay 3 seconds
@15:31:43.33 ATV.dial :bleach
@15:31:44.00 AS.seek 0.82ml
@15:31:47.31 delay 3 seconds
@15:31:50.33 ATV.dial PCR
@15:31:50.78 AS.reconfigure ASconfig
@15:31:50.85 AS.seek 0ml
@15:32:38.26 PCR.program :bleachProfile
@15:32:38.34 PCR program should complete in < 2:45
@15:32:38.35 PCR.run 10:00
@15:35:34.16 PCR.off
@15:35:34.21 AFV.select :carrierSolution
@15:35:34.33 delay 2 seconds
@15:35:36.36 AS.seek 1ml
@15:36:34.15 delay 1 second
@15:36:35.17 AFV.select :holdingCoil
@15:36:35.27 delay 1 second
@15:36:36.30 AS.reconfigure ASfast
@15:36:36.36 AS.seek 0ml
@15:37:03.73 delay 3 seconds
@15:37:06.75 AFV.select :carrierSolution
@15:37:06.85 delay 2 seconds
@15:37:08.88 AS.reconfigure ASconfig
@15:37:08.95 AS.seek 1ml
@15:38:06.72 delay 1 second
@15:38:07.74 AFV.select :holdingCoil
@15:38:07.84 delay 1 second
@15:38:08.87 AS.reconfigure ASfast
@15:38:08.93 AS.seek 0ml
@15:38:36.28 delay 3 seconds
@15:38:39.30 AFV.select :carrierSolution
@15:38:39.40 delay 2 seconds
@15:38:41.43 AS.reconfigure ASconfig
@15:38:41.50 AS.seek 1ml
@15:39:39.25 delay 1 second
@15:39:40.27 AFV.select :holdingCoil
@15:39:40.37 delay 1 second
@15:39:41.40 AS.reconfigure ASfast
@15:39:41.46 AS.seek 0ml
@15:40:08.83 delay 3 seconds
@15:40:11.85 AFV.select :carrierSolution
@15:40:11.95 delay 2 seconds
@15:40:13.98 AS.reconfigure ASconfig
@15:40:14.05 AS.seek 1ml
@15:41:11.81 delay 1 second
@15:41:12.83 AFV.select :holdingCoil
@15:41:12.93 delay 1 second
@15:41:13.96 AS.reconfigure ASfast
@15:41:14.02 AS.seek 0ml
@15:41:41.37 delay 3 seconds
@15:41:44.39 AFV.select :carrierSolution
@15:41:44.49 delay 2 seconds
@15:41:46.51 AS.reconfigure ASconfig
@15:41:46.58 AS.seek 1ml
@15:42:44.33 delay 1 second
@15:42:45.35 AFV.select :holdingCoil
@15:42:45.45 delay 1 second
@15:42:46.48 AS.reconfigure ASnorm
@15:42:46.54 AS.seek 0ml
@15:43:37.28 <MAIN> AFV.select :carrierSolution
@15:43:37.40 delay 2 seconds
@15:43:39.43 AS.reconfigure ASconfig
@15:43:39.49 AS.seek 0.5ml
@15:44:08.51 delay 1 second
@15:44:09.53 AFV.select :holdingCoil
@15:44:09.63 ATV.dial :air1
@15:44:11.21 AS.reconfigure ASslow2
@15:44:11.28 AS.seek 0.506ml
@15:44:17.90 ATV.dial :extSrc
@15:44:19.69 AS.seek 0.512ml
@15:44:26.08 delay 5 seconds
@15:44:31.10 ATV.dial :air1
@15:44:32.92 AS.reconfigure ASslow1
@15:44:32.99 AS.seek 0.542ml
@15:44:39.31 ATV.dial :waste
@15:44:39.89 AS.reconfigure ASconfig
@15:44:39.96 AS.seek 0ml
@15:45:11.92 <MFBPCR> Setting up PCR for AARPP4 -- writing /var/log/esp/AARPP421apr2815h.pcr
@15:45:11.95 Building PCR Slug from extSrc with MM2 mix, PP4 primer
@15:45:11.98 Priming with carrier
@15:45:12.01 ATV.dial ARV
@15:45:13.62 ARV.dial :carrier
@15:45:13.98 AS.reconfigure ASnorm
@15:45:14.05 AS.seek 0.5ml
@15:45:39.65 AFV.select :carrierSolution
@15:45:39.78 delay 2 seconds
@15:45:41.81 AS.reconfigure ASconfig
@15:45:41.88 AS.seek 1ml
@15:46:10.94 delay 1 second
@15:46:11.96 AFV.select :holdingCoil
@15:46:12.08 delay 1 second
@15:46:13.10 ATV.dial :waste
@15:46:14.87 AS.reconfigure ASnorm
@15:46:14.95 AS.seek 0ml
@15:47:05.69 AFV.select :carrierSolution
@15:47:05.81 delay 2 seconds
@15:47:07.84 AS.reconfigure ASconfig
@15:47:07.90 AS.seek 0.5ml
@15:47:36.95 delay 1 second
@15:47:37.97 AFV.select :holdingCoil
@15:47:38.07 Building Slug
@15:47:38.08 ATV.dial :ARV
@15:47:39.87 delay 2 seconds
@15:47:41.89 ARV.dial :air1
@15:47:42.31 delay 2 seconds
@15:47:44.38 AS.reconfigure ASslow2
@15:47:44.45 AS.seek 0.506ml
@15:47:50.86 delay 2 seconds
@15:47:52.88 ARV.dial :MM2
@15:47:54.47 delay 2 seconds
@15:47:56.49 Adding MM2
@15:47:56.58 AS.seek 0.524ml
@15:48:14.94 delay 25 seconds
@15:48:39.96 ARV.dial :PP4
@15:48:40.41 Adding PP4
@15:48:40.42 delay 2 seconds
@15:48:42.52 AS.seek 0.53ml
@15:48:49.00 delay 10 seconds
@15:48:59.03 ARV.dial :air3
@15:48:59.44 adding ARV air
@15:48:59.45 delay 2 seconds
@15:49:01.54 AS.seek 0.536ml
@15:49:07.97 delay 5 seconds
@15:49:12.99 ARV.dial :carrier
@15:49:14.36 delay 7 seconds
@15:49:21.44 AS.seek 0.594ml
@15:50:19.97 delay 5 seconds
@15:50:24.99 ATV.dial :extSrc
@15:50:25.61 delay 2 seconds
@15:50:27.68 AS.seek 0.6ml
@15:50:33.97 delay 4 seconds
@15:50:37.99 ATV.dial ARV
@15:50:38.58 delay 2 seconds
@15:50:40.60 ARV.dial :carrier
@15:50:40.61 delay 2 seconds
@15:50:42.71 AS.seek 0.65ml
@15:51:33.15 delay 4 seconds
@15:51:37.17 ATV.dial PCR
@15:51:37.98 ASV.dial :waste1
@15:51:37.99 ARV.dial :air1
@15:51:38.43 Positioning Slug by 0.324ml
@15:51:38.53 AS.seek 0.326ml
@15:57:05.03 delay 5 seconds
@15:57:10.05 ATV.dial ARV
@15:57:10.87 ASV.dial :PCRlock
@15:57:11.28 delay 5 seconds
@15:57:16.30 Running AARPP4 Profile58CIQ2 thermocycling
@15:57:16.31 <PCR> PCR.program Profile58CIQ2
@15:57:16.45 Profile58CIQ2 should complete in < 1:36:15
@15:57:16.46 PCR.run 2:05:18.75
@15:58:15.21 <bkhaa> -> exit
@15:58:15.23 Session Ended
@17:18:46.96 <PCR> PCR.off
@17:18:47.52 <MFBPCR> Running AARPP4 MeltProfile58 thermocycling
@17:18:47.53 <PCR> PCR.program MeltProfile58
@17:18:47.63 MeltProfile58 should complete in < 12:33
@17:18:47.64 PCR.run 20:41.25
@17:26:50.71 PCR.off
@17:26:51.67 <MFBPCR> PCR for AARPP4 completed -- closed /var/log/esp/AARPP421apr2815h.pcr
@17:26:51.74 Cleaning PCR
@17:26:51.75 AFV.select :carrierSolution
@17:26:51.87 delay 2 seconds
@17:26:53.90 AS.reconfigure ASconfig
@17:26:53.95 AS.seek 0.5ml
@17:27:04.28 delay 1 second
@17:27:05.30 AFV.select :holdingCoil
@17:27:05.40 ASV.dial :waste1
@17:27:05.81 ATV.dial :air3
@17:27:07.16 AS.reconfigure ASslow1
@17:27:07.22 AS.seek 0.51ml
@17:27:09.50 delay 2 seconds
@17:27:11.52 ATV.dial :bleach
@17:27:12.16 AS.seek 0.81ml
@17:28:12.79 delay 3 seconds
@17:28:15.81 ATV.dial :air3
@17:28:16.40 delay 3 seconds
@17:28:19.47 AS.seek 0.835ml
@17:28:24.75 delay 3 seconds
@17:28:27.77 ATV.dial :bleach
@17:28:28.42 AS.seek 0.82ml
@17:28:31.84 delay 3 seconds
@17:28:34.86 ARV.dial :waste
@17:28:35.51 delay 3 seconds
@17:28:38.53 ATV.dial :ARV
@17:28:39.47 AS.reconfigure ASconfig
@17:28:39.90 AS.seek 0.47ml
@17:29:00.34 AFV.select :carrierSolution
@17:29:00.45 delay 2 seconds
@17:29:02.48 AS.seek 1ml
@17:29:33.23 delay 1 second
@17:29:34.25 AFV.select :holdingCoil
@17:29:34.35 delay 1 second
@17:29:35.38 AS.reconfigure ASfast
@17:29:35.43 AS.seek 0ml
@17:30:02.81 AFV.select :carrierSolution
@17:30:02.95 delay 2 seconds
@17:30:04.99 AS.reconfigure ASconfig
@17:30:05.04 AS.seek 1ml
@17:31:02.78 delay 1 second
@17:31:03.80 AFV.select :holdingCoil
@17:31:03.90 delay 1 second
@17:31:04.93 AS.reconfigure ASfast
@17:31:04.98 AS.seek 0ml
@17:31:32.32 AFV.select :carrierSolution
@17:31:32.43 delay 2 seconds
@17:31:34.46 AS.reconfigure ASconfig
@17:31:34.51 AS.seek 1ml
@17:32:32.26 delay 1 second
@17:32:33.28 AFV.select :holdingCoil
@17:32:33.38 delay 1 second
@17:32:34.41 AS.reconfigure ASfast
@17:32:34.46 AS.seek 0ml
@17:33:01.78 ARV.dial between carrier and air1
@17:33:02.31 ATV.dial :air3
@17:33:03.67 AS.reconfigure ASslow1
@17:33:03.73 AS.seek 0.01ml
@17:33:06.04 delay 2 seconds
@17:33:08.06 ATV.dial :bleach
@17:33:08.73 AS.seek 0.81ml
@17:35:50.00 delay 3 seconds
@17:35:53.02 ATV.dial :air3
@17:35:53.62 delay 3 seconds
@17:35:56.69 AS.seek 0.835ml
@17:36:02.02 delay 3 seconds
@17:36:05.04 ATV.dial :bleach
@17:36:05.71 AS.seek 0.82ml
@17:36:09.11 delay 3 seconds
@17:36:12.13 ATV.dial PCR
@17:36:12.54 AS.reconfigure ASconfig
@17:36:12.60 AS.seek 0ml
@17:37:00.03 PCR.program :bleachProfile
@17:37:00.09 PCR program should complete in < 2:45
@17:37:00.11 PCR.run 10:00
@17:39:55.90 PCR.off
@17:39:55.94 AFV.select :carrierSolution
@17:39:56.05 delay 2 seconds
@17:39:58.08 AS.seek 1ml
@17:40:55.81 delay 1 second
@17:40:56.83 AFV.select :holdingCoil
@17:40:56.93 delay 1 second
@17:40:57.96 AS.reconfigure ASfast
@17:40:58.01 AS.seek 0ml
@17:41:25.34 delay 3 seconds
@17:41:28.36 AFV.select :carrierSolution
@17:41:28.47 delay 2 seconds
@17:41:30.49 AS.reconfigure ASconfig
@17:41:30.55 AS.seek 1ml
@17:42:28.31 delay 1 second
@17:42:29.33 AFV.select :holdingCoil
@17:42:29.43 delay 1 second
@17:42:30.46 AS.reconfigure ASfast
@17:42:30.51 AS.seek 0ml
@17:42:57.86 delay 3 seconds
@17:43:00.88 AFV.select :carrierSolution
@17:43:00.98 delay 2 seconds
@17:43:03.00 AS.reconfigure ASconfig
@17:43:03.07 AS.seek 1ml
@17:44:00.81 delay 1 second
@17:44:01.83 AFV.select :holdingCoil
@17:44:01.93 delay 1 second
@17:44:02.96 AS.reconfigure ASfast
@17:44:03.01 AS.seek 0ml
@17:44:30.36 delay 3 seconds
@17:44:33.38 AFV.select :carrierSolution
@17:44:33.48 delay 2 seconds
@17:44:35.50 AS.reconfigure ASconfig
@17:44:35.56 AS.seek 1ml
@17:45:33.29 delay 1 second
@17:45:34.31 AFV.select :holdingCoil
@17:45:34.41 delay 1 second
@17:45:35.44 AS.reconfigure ASfast
@17:45:35.49 AS.seek 0ml
@17:46:02.86 delay 3 seconds
@17:46:05.88 AFV.select :carrierSolution
@17:46:05.98 delay 2 seconds
@17:46:08.00 AS.reconfigure ASconfig
@17:46:08.07 AS.seek 1ml
@17:47:05.86 delay 1 second
@17:47:06.88 AFV.select :holdingCoil
@17:47:06.98 delay 1 second
@17:47:08.01 AS.reconfigure ASnorm
@17:47:08.45 AS.seek 0ml
@17:47:59.19 <MAIN> AFV.select :carrierSolution
@17:47:59.29 delay 2 seconds
@17:48:01.32 AS.reconfigure ASconfig
@17:48:01.37 AS.seek 0.5ml
@17:48:30.45 delay 1 second
@17:48:31.47 AFV.select :holdingCoil
@17:48:31.57 ATV.dial :air1
@17:48:33.14 AS.reconfigure ASslow2
@17:48:33.20 AS.seek 0.51ml
@17:48:43.39 delay 5 seconds
@17:48:48.41 ATV.dial :extSrc
@17:48:50.20 AS.seek 0.53ml
@17:49:10.86 delay 10 seconds
@17:49:20.89 ATV.dial :air1
@17:49:22.69 AS.reconfigure ASslow1
@17:49:22.75 AS.seek 0.56ml
@17:49:29.14 ATV.dial :waste
@17:49:29.71 AS.reconfigure ASconfig
@17:49:29.77 AS.seek 0ml
@17:50:02.38 <MFBPCR> Setting up PCR for AARPP4 -- writing /var/log/esp/AARPP421apr2817h.pcr
@17:50:02.41 Building PCR Slug from extSrc with MM2 mix, PP4 primer
@17:50:02.43 Priming with carrier
@17:50:02.45 ATV.dial ARV
@17:50:04.06 ARV.dial :carrier
@17:50:04.45 AS.reconfigure ASnorm
@17:50:04.51 AS.seek 0.5ml
@17:50:30.07 AFV.select :carrierSolution
@17:50:30.19 delay 2 seconds
@17:50:32.22 AS.reconfigure ASconfig
@17:50:32.28 AS.seek 1ml
@17:51:01.31 delay 1 second
@17:51:02.33 AFV.select :holdingCoil
@17:51:02.43 delay 1 second
@17:51:03.45 ATV.dial :waste
@17:51:05.21 AS.reconfigure ASnorm
@17:51:05.27 AS.seek 0ml
@17:51:56.00 AFV.select :carrierSolution
@17:51:56.12 delay 2 seconds
@17:51:58.15 AS.reconfigure ASconfig
@17:51:58.20 AS.seek 0.5ml
@17:52:27.25 delay 1 second
@17:52:28.27 AFV.select :holdingCoil
@17:52:28.37 Building Slug
@17:52:28.39 ATV.dial :ARV
@17:52:30.14 delay 2 seconds
@17:52:32.16 ARV.dial :air1
@17:52:32.56 delay 2 seconds
@17:52:34.63 AS.reconfigure ASslow2
@17:52:34.69 AS.seek 0.506ml
@17:52:41.14 delay 2 seconds
@17:52:43.16 ARV.dial :MM2
@17:52:44.75 delay 2 seconds
@17:52:46.77 Adding MM2
@17:52:46.85 AS.seek 0.524ml
@17:53:05.50 delay 25 seconds
@17:53:30.52 ARV.dial :PP4
@17:53:30.93 Adding PP4
@17:53:30.94 delay 2 seconds
@17:53:33.02 AS.seek 0.53ml
@17:53:39.71 delay 10 seconds
@17:53:49.74 ARV.dial :air3
@17:53:50.15 adding ARV air
@17:53:50.16 delay 2 seconds
@17:53:52.24 AS.seek 0.536ml
@17:53:58.59 delay 5 seconds
@17:54:03.61 ARV.dial :carrier
@17:54:04.95 delay 7 seconds
@17:54:12.03 AS.seek 0.594ml
@17:55:10.87 delay 5 seconds
@17:55:15.89 ATV.dial :extSrc
@17:55:16.50 delay 2 seconds
@17:55:18.57 AS.seek 0.6ml
@17:55:25.26 delay 4 seconds
@17:55:29.28 ATV.dial ARV
@17:55:29.87 delay 2 seconds
@17:55:31.89 ARV.dial :carrier
@17:55:31.90 delay 2 seconds
@17:55:33.98 AS.seek 0.65ml
@17:56:24.50 delay 4 seconds
@17:56:28.52 ATV.dial PCR
@17:56:29.32 ASV.dial :waste1
@17:56:29.33 ARV.dial :air1
@17:56:29.77 Positioning Slug by 0.324ml
@17:56:29.86 AS.seek 0.326ml
@18:01:56.48 delay 5 seconds
@18:02:01.50 ATV.dial ARV
@18:02:02.32 ASV.dial :PCRlock
@18:02:02.71 delay 5 seconds
@18:02:07.73 Running AARPP4 Profile58CIQ2 thermocycling
@18:02:07.75 <PCR> PCR.program Profile58CIQ2
@18:02:07.86 Profile58CIQ2 should complete in < 1:36:15
@18:02:07.87 PCR.run 2:05:18.75
@19:23:19.79 PCR.off
@19:23:20.34 <MFBPCR> Running AARPP4 MeltProfile58 thermocycling
@19:23:20.36 <PCR> PCR.program MeltProfile58
@19:23:20.46 MeltProfile58 should complete in < 12:33
@19:23:20.47 PCR.run 20:41.25
@19:31:14.90 PCR.off
@19:31:15.88 <MFBPCR> PCR for AARPP4 completed -- closed /var/log/esp/AARPP421apr2817h.pcr
@19:31:15.95 Cleaning PCR
@19:31:15.96 AFV.select :carrierSolution
@19:31:16.08 delay 2 seconds
@19:31:18.11 AS.reconfigure ASconfig
@19:31:18.16 AS.seek 0.5ml
@19:31:28.48 delay 1 second
@19:31:29.50 AFV.select :holdingCoil
@19:31:29.60 ASV.dial :waste1
@19:31:29.99 ATV.dial :air3
@19:31:31.36 AS.reconfigure ASslow1
@19:31:31.43 AS.seek 0.51ml
@19:31:33.71 delay 2 seconds
@19:31:35.73 ATV.dial :bleach
@19:31:36.40 AS.seek 0.81ml
@19:32:36.99 delay 3 seconds
@19:32:40.01 ATV.dial :air3
@19:32:40.60 delay 3 seconds
@19:32:43.67 AS.seek 0.835ml
@19:32:48.96 delay 3 seconds
@19:32:51.98 ATV.dial :bleach
@19:32:52.62 AS.seek 0.82ml
@19:32:56.10 delay 3 seconds
@19:32:59.12 ARV.dial :waste
@19:32:59.78 delay 3 seconds
@19:33:02.80 ATV.dial :ARV
@19:33:03.72 AS.reconfigure ASconfig
@19:33:03.78 AS.seek 0.47ml
@19:33:24.20 AFV.select :carrierSolution
@19:33:24.31 delay 2 seconds
@19:33:26.34 AS.seek 1ml
@19:33:57.09 delay 1 second
@19:33:58.11 AFV.select :holdingCoil
@19:33:58.21 delay 1 second
@19:33:59.24 AS.reconfigure ASfast
@19:33:59.29 AS.seek 0ml
@19:34:26.65 AFV.select :carrierSolution
@19:34:26.76 delay 2 seconds
@19:34:28.79 AS.reconfigure ASconfig
@19:34:28.84 AS.seek 1ml
@19:35:26.60 delay 1 second
@19:35:27.62 AFV.select :holdingCoil
@19:35:27.72 delay 1 second
@19:35:28.75 AS.reconfigure ASfast
@19:35:28.80 AS.seek 0ml
@19:35:56.12 AFV.select :carrierSolution
@19:35:56.23 delay 2 seconds
@19:35:58.26 AS.reconfigure ASconfig
@19:35:58.31 AS.seek 1ml
@19:36:56.06 delay 1 second
@19:36:57.08 AFV.select :holdingCoil
@19:36:57.18 delay 1 second
@19:36:58.21 AS.reconfigure ASfast
@19:36:58.26 AS.seek 0ml
@19:37:25.59 ARV.dial between carrier and air1
@19:37:26.10 ATV.dial :air3
@19:37:27.45 AS.reconfigure ASslow1
@19:37:27.51 AS.seek 0.01ml
@19:37:29.93 delay 2 seconds
@19:37:31.95 ATV.dial :bleach
@19:37:32.61 AS.seek 0.81ml
@19:40:13.90 delay 3 seconds
@19:40:16.92 ATV.dial :air3
@19:40:17.51 delay 3 seconds
@19:40:20.58 AS.seek 0.835ml
@19:40:25.91 delay 3 seconds
@19:40:28.93 ATV.dial :bleach
@19:40:29.58 AS.seek 0.82ml
@19:40:32.90 delay 3 seconds
@19:40:35.92 ATV.dial PCR
@19:40:36.34 AS.reconfigure ASconfig
@19:40:36.40 AS.seek 0ml
@19:41:23.82 PCR.program :bleachProfile
@19:41:23.89 PCR program should complete in < 2:45
@19:41:23.90 PCR.run 10:00
@19:44:20.06 PCR.off
@19:44:20.10 AFV.select :carrierSolution
@19:44:20.21 delay 2 seconds
@19:44:22.24 AS.seek 1ml
@19:45:20.03 delay 1 second
@19:45:21.05 AFV.select :holdingCoil
@19:45:21.15 delay 1 second
@19:45:22.18 AS.reconfigure ASfast
@19:45:22.23 AS.seek 0ml
@19:45:49.57 delay 3 seconds
@19:45:52.59 AFV.select :carrierSolution
@19:45:52.69 delay 2 seconds
@19:45:54.72 AS.reconfigure ASconfig
@19:45:54.78 AS.seek 1ml
@19:46:52.57 delay 1 second
@19:46:53.59 AFV.select :holdingCoil
@19:46:53.69 delay 1 second
@19:46:54.72 AS.reconfigure ASfast
@19:46:54.77 AS.seek 0ml
@19:47:22.12 delay 3 seconds
@19:47:25.14 AFV.select :carrierSolution
@19:47:25.24 delay 2 seconds
@19:47:27.27 AS.reconfigure ASconfig
@19:47:27.33 AS.seek 1ml
@19:48:25.06 delay 1 second
@19:48:26.08 AFV.select :holdingCoil
@19:48:26.18 delay 1 second
@19:48:27.21 AS.reconfigure ASfast
@19:48:27.26 AS.seek 0ml
@19:48:54.62 delay 3 seconds
@19:48:57.64 AFV.select :carrierSolution
@19:48:57.74 delay 2 seconds
@19:48:59.77 AS.reconfigure ASconfig
@19:48:59.83 AS.seek 1ml
@19:49:57.57 delay 1 second
@19:49:58.59 AFV.select :holdingCoil
@19:49:58.69 delay 1 second
@19:49:59.72 AS.reconfigure ASfast
@19:49:59.77 AS.seek 0ml
@19:50:27.10 delay 3 seconds
@19:50:30.12 AFV.select :carrierSolution
@19:50:30.22 delay 2 seconds
@19:50:32.24 AS.reconfigure ASconfig
@19:50:32.34 AS.seek 1ml
@19:51:30.09 delay 1 second
@19:51:31.11 AFV.select :holdingCoil
@19:51:31.21 delay 1 second
@19:51:32.24 AS.reconfigure ASnorm
@19:51:32.29 AS.seek 0ml
@19:52:23.03 <MAIN> AFV.select :carrierSolution
@19:52:23.14 delay 2 seconds
@19:52:25.17 AS.reconfigure ASconfig
@19:52:25.22 AS.seek 0.5ml
@19:52:54.24 delay 1 second
@19:52:55.26 AFV.select :holdingCoil
@19:52:55.36 ATV.dial :air1
@19:52:56.93 AS.reconfigure ASslow2
@19:52:56.99 AS.seek 0.506ml
@19:53:03.57 ATV.dial :extSrc
@19:53:05.34 AS.seek 0.512ml
@19:53:11.67 delay 5 seconds
@19:53:16.69 ATV.dial :air1
@19:53:18.50 AS.reconfigure ASslow1
@19:53:18.56 AS.seek 0.542ml
@19:53:24.92 ATV.dial :waste
@19:53:25.49 AS.reconfigure ASconfig
@19:53:25.55 AS.seek 0ml
@19:53:57.17 <MFBPCR> Setting up PCR for AARPP4 -- writing /var/log/esp/AARPP421apr2819h.pcr
@19:53:57.20 Building PCR Slug from extSrc with MM2 mix, PP4 primer
@19:53:57.22 Priming with carrier
@19:53:57.24 ATV.dial ARV
@19:53:58.82 ARV.dial :carrier
@19:53:59.18 AS.reconfigure ASnorm
@19:53:59.24 AS.seek 0.5ml
@19:54:24.85 AFV.select :carrierSolution
@19:54:24.97 delay 2 seconds
@19:54:27.00 AS.reconfigure ASconfig
@19:54:27.05 AS.seek 1ml
@19:54:56.07 delay 1 second
@19:54:57.09 AFV.select :holdingCoil
@19:54:57.19 delay 1 second
@19:54:58.21 ATV.dial :waste
@19:54:59.99 AS.reconfigure ASnorm
@19:55:00.06 AS.seek 0ml
@19:55:50.73 AFV.select :carrierSolution
@19:55:50.84 delay 2 seconds
@19:55:52.87 AS.reconfigure ASconfig
@19:55:52.92 AS.seek 0.5ml
@19:56:21.99 delay 1 second
@19:56:23.01 AFV.select :holdingCoil
@19:56:23.11 Building Slug
@19:56:23.12 ATV.dial :ARV
@19:56:24.87 delay 2 seconds
@19:56:26.89 ARV.dial :air1
@19:56:27.30 delay 2 seconds
@19:56:29.42 AS.reconfigure ASslow2
@19:56:29.51 AS.seek 0.506ml
@19:56:36.70 delay 2 seconds
@19:56:38.72 ARV.dial :MM2
@19:56:40.31 delay 2 seconds
@19:56:42.33 Adding MM2
@19:56:42.45 AS.seek 0.524ml
@19:57:00.98 delay 25 seconds
@19:57:26.00 ARV.dial :PP4
@19:57:26.42 Adding PP4
@19:57:26.43 delay 2 seconds
@19:57:28.51 AS.seek 0.53ml
@19:57:35.09 delay 10 seconds
@19:57:45.12 ARV.dial :air3
@19:57:45.54 adding ARV air
@19:57:45.55 delay 2 seconds
@19:57:47.63 AS.seek 0.536ml
@19:57:54.34 delay 5 seconds
@19:57:59.36 ARV.dial :carrier
@19:58:00.70 delay 7 seconds
@19:58:07.78 AS.seek 0.594ml
@19:59:06.24 delay 5 seconds
@19:59:11.26 ATV.dial :extSrc
@19:59:11.84 delay 2 seconds
@19:59:13.91 AS.seek 0.6ml
@19:59:20.17 delay 4 seconds
@19:59:24.19 ATV.dial ARV
@19:59:24.78 delay 2 seconds
@19:59:26.80 ARV.dial :carrier
@19:59:26.81 delay 2 seconds
@19:59:28.89 AS.seek 0.65ml
@20:00:19.32 delay 4 seconds
@20:00:23.34 ATV.dial PCR
@20:00:24.15 ASV.dial :waste1
@20:00:24.16 ARV.dial :air1
@20:00:24.59 Positioning Slug by 0.324ml
@20:00:24.68 AS.seek 0.326ml
@20:05:50.73 delay 5 seconds
@20:05:55.75 ATV.dial ARV
@20:05:56.60 ASV.dial :PCRlock
@20:05:57.01 delay 5 seconds
@20:06:02.03 Running AARPP4 Profile58CIQ2 thermocycling
@20:06:02.04 <PCR> PCR.program Profile58CIQ2
@20:06:02.17 Profile58CIQ2 should complete in < 1:36:15
@20:06:02.18 PCR.run 2:05:18.75
@21:27:10.76 PCR.off
@21:27:11.33 <MFBPCR> Running AARPP4 MeltProfile58 thermocycling
@21:27:11.34 <PCR> PCR.program MeltProfile58
@21:27:11.44 MeltProfile58 should complete in < 12:33
@21:27:11.45 PCR.run 20:41.25
@21:34:59.01 PCR.off
@21:34:59.98 <MFBPCR> PCR for AARPP4 completed -- closed /var/log/esp/AARPP421apr2819h.pcr
@21:35:00.06 Cleaning PCR
@21:35:00.07 AFV.select :carrierSolution
@21:35:00.18 delay 2 seconds
@21:35:02.21 AS.reconfigure ASconfig
@21:35:02.26 AS.seek 0.5ml
@21:35:12.57 delay 1 second
@21:35:13.59 AFV.select :holdingCoil
@21:35:13.69 ASV.dial :waste1
@21:35:14.09 ATV.dial :air3
@21:35:15.47 AS.reconfigure ASslow1
@21:35:15.53 AS.seek 0.51ml
@21:35:17.92 delay 2 seconds
@21:35:19.94 ATV.dial :bleach
@21:35:20.59 AS.seek 0.81ml
@21:36:21.31 delay 3 seconds
@21:36:24.33 ATV.dial :air3
@21:36:24.92 delay 3 seconds
@21:36:27.99 AS.seek 0.835ml
@21:36:33.42 delay 3 seconds
@21:36:36.44 ATV.dial :bleach
@21:36:37.07 AS.seek 0.82ml
@21:36:40.49 delay 3 seconds
@21:36:43.51 ARV.dial :waste
@21:36:44.17 delay 3 seconds
@21:36:47.19 ATV.dial :ARV
@21:36:48.12 AS.reconfigure ASconfig
@21:36:48.18 AS.seek 0.47ml
@21:37:08.57 AFV.select :carrierSolution
@21:37:08.68 delay 2 seconds
@21:37:10.71 AS.seek 1ml
@21:37:41.45 delay 1 second
@21:37:42.47 AFV.select :holdingCoil
@21:37:42.57 delay 1 second
@21:37:43.60 AS.reconfigure ASfast
@21:37:43.65 AS.seek 0ml
@21:38:10.99 AFV.select :carrierSolution
@21:38:11.10 delay 2 seconds
@21:38:13.13 AS.reconfigure ASconfig
@21:38:13.56 AS.seek 1ml
@21:39:11.32 delay 1 second
@21:39:12.34 AFV.select :holdingCoil
@21:39:12.44 delay 1 second
@21:39:13.47 AS.reconfigure ASfast
@21:39:13.52 AS.seek 0ml
@21:39:40.87 AFV.select :carrierSolution
@21:39:40.98 delay 2 seconds
@21:39:43.01 AS.reconfigure ASconfig
@21:39:43.06 AS.seek 1ml
@21:40:40.82 delay 1 second
@21:40:41.84 AFV.select :holdingCoil
@21:40:41.94 delay 1 second
@21:40:42.97 AS.reconfigure ASfast
@21:40:43.02 AS.seek 0ml
@21:41:10.35 ARV.dial between carrier and air1
@21:41:10.87 ATV.dial :air3
@21:41:12.24 AS.reconfigure ASslow1
@21:41:12.29 AS.seek 0.01ml
@21:41:14.70 delay 2 seconds
@21:41:16.72 ATV.dial :bleach
@21:41:17.38 AS.seek 0.81ml
@21:43:58.52 delay 3 seconds
@21:44:01.54 ATV.dial :air3
@21:44:02.13 delay 3 seconds
@21:44:05.20 AS.seek 0.835ml
@21:44:10.48 delay 3 seconds
@21:44:13.50 ATV.dial :bleach
@21:44:14.15 AS.seek 0.82ml
@21:44:17.45 delay 3 seconds
@21:44:20.47 ATV.dial PCR
@21:44:20.90 AS.reconfigure ASconfig
@21:44:20.96 AS.seek 0ml
@21:45:08.37 PCR.program :bleachProfile
@21:45:08.43 PCR program should complete in < 2:45
@21:45:08.44 PCR.run 10:00
@21:48:04.23 PCR.off
@21:48:04.27 AFV.select :carrierSolution
@21:48:04.38 delay 2 seconds
@21:48:06.41 AS.seek 1ml
@21:49:04.16 delay 1 second
@21:49:05.18 AFV.select :holdingCoil
@21:49:05.28 delay 1 second
@21:49:06.31 AS.reconfigure ASfast
@21:49:06.36 AS.seek 0ml
@21:49:33.70 delay 3 seconds
@21:49:36.72 AFV.select :carrierSolution
@21:49:36.82 delay 2 seconds
@21:49:38.84 AS.reconfigure ASconfig
@21:49:38.91 AS.seek 1ml
@21:50:36.65 delay 1 second
@21:50:37.67 AFV.select :holdingCoil
@21:50:37.77 delay 1 second
@21:50:38.80 AS.reconfigure ASfast
@21:50:38.85 AS.seek 0ml
@21:51:06.21 delay 3 seconds
@21:51:09.23 AFV.select :carrierSolution
@21:51:09.33 delay 2 seconds
@21:51:11.36 AS.reconfigure ASconfig
@21:51:11.43 AS.seek 1ml
@21:52:09.16 delay 1 second
@21:52:10.18 AFV.select :holdingCoil
@21:52:10.28 delay 1 second
@21:52:11.31 AS.reconfigure ASfast
@21:52:11.36 AS.seek 0ml
@21:52:39.07 delay 3 seconds
@21:52:42.09 AFV.select :carrierSolution
@21:52:42.19 delay 2 seconds
@21:52:44.22 AS.reconfigure ASconfig
@21:52:44.28 AS.seek 1ml
@21:53:42.02 delay 1 second
@21:53:43.04 AFV.select :holdingCoil
@21:53:43.14 delay 1 second
@21:53:44.17 AS.reconfigure ASfast
@21:53:44.22 AS.seek 0ml
@21:54:11.57 delay 3 seconds
@21:54:14.59 AFV.select :carrierSolution
@21:54:14.69 delay 2 seconds
@21:54:16.72 AS.reconfigure ASconfig
@21:54:16.78 AS.seek 1ml
@21:55:14.56 delay 1 second
@21:55:15.58 AFV.select :holdingCoil
@21:55:15.68 delay 1 second
@21:55:16.71 AS.reconfigure ASnorm
@21:55:16.76 AS.seek 0ml
@21:56:07.50 <MAIN> AFV.select :carrierSolution
@21:56:07.60 delay 2 seconds
@21:56:09.63 AS.reconfigure ASconfig
@21:56:09.68 AS.seek 0.5ml
@21:56:38.73 delay 1 second
@21:56:39.75 AFV.select :holdingCoil
@21:56:39.85 ATV.dial :air1
@21:56:41.43 AS.reconfigure ASslow1
@21:56:41.49 AS.seek 0.52ml
@21:56:45.79 delay 5 seconds
@21:56:50.81 ATV.dial :extSrc
@21:56:52.59 AS.reconfigure ASslow2
@21:56:52.65 AS.seek 0.545ml
@21:57:18.32 delay 10 seconds
@21:57:28.35 ATV.dial :air1
@21:57:30.15 AS.reconfigure ASslow1
@21:57:30.21 AS.seek 0.575ml
@21:57:36.49 ATV.dial :waste
@21:57:37.06 AS.reconfigure ASconfig
@21:57:37.12 AS.seek 0ml
@21:58:10.60 <MFBPCR> Setting up PCR for AARPP4 -- writing /var/log/esp/AARPP421apr2821h.pcr
@21:58:10.62 Building PCR Slug from extSrc with MM2 mix, PP4 primer
@21:58:10.65 Priming with carrier
@21:58:10.67 ATV.dial ARV
@21:58:12.27 ARV.dial :carrier
@21:58:12.62 AS.reconfigure ASnorm
@21:58:12.68 AS.seek 0.5ml
@21:58:38.26 AFV.select :carrierSolution
@21:58:38.37 delay 2 seconds
@21:58:40.40 AS.reconfigure ASconfig
@21:58:40.45 AS.seek 1ml
@21:59:09.46 delay 1 second
@21:59:10.48 AFV.select :holdingCoil
@21:59:10.58 delay 1 second
@21:59:11.60 ATV.dial :waste
@21:59:13.38 AS.reconfigure ASnorm
@21:59:13.44 AS.seek 0ml
@22:00:04.15 AFV.select :carrierSolution
@22:00:04.26 delay 2 seconds
@22:00:06.29 AS.reconfigure ASconfig
@22:00:06.34 AS.seek 0.5ml
@22:00:35.40 delay 1 second
@22:00:36.42 AFV.select :holdingCoil
@22:00:36.52 Building Slug
@22:00:36.54 ATV.dial :ARV
@22:00:38.40 delay 2 seconds
@22:00:40.42 ARV.dial :air1
@22:00:40.84 delay 2 seconds
@22:00:42.91 AS.reconfigure ASslow2
@22:00:42.97 AS.seek 0.506ml
@22:00:49.18 delay 2 seconds
@22:00:51.20 ARV.dial :MM2
@22:00:52.79 delay 2 seconds
@22:00:54.81 Adding MM2
@22:00:54.89 AS.seek 0.524ml
@22:01:13.57 delay 25 seconds
@22:01:38.59 ARV.dial :PP4
@22:01:39.01 Adding PP4
@22:01:39.02 delay 2 seconds
@22:01:41.10 AS.seek 0.53ml
@22:01:47.36 delay 10 seconds
@22:01:57.40 ARV.dial :air3
@22:01:57.89 adding ARV air
@22:01:57.91 delay 2 seconds
@22:02:00.00 AS.seek 0.536ml
@22:02:06.35 delay 5 seconds
@22:02:11.37 ARV.dial :carrier
@22:02:12.70 delay 7 seconds
@22:02:19.78 AS.seek 0.594ml
@22:03:18.51 delay 5 seconds
@22:03:23.53 ATV.dial :extSrc
@22:03:24.13 delay 2 seconds
@22:03:26.20 AS.seek 0.6ml
@22:03:32.63 delay 4 seconds
@22:03:36.65 ATV.dial ARV
@22:03:37.23 delay 2 seconds
@22:03:39.25 ARV.dial :carrier
@22:03:39.26 delay 2 seconds
@22:03:41.34 AS.seek 0.65ml
@22:04:32.31 delay 4 seconds
@22:04:36.33 ATV.dial PCR
@22:04:37.13 ASV.dial :waste1
@22:04:37.14 ARV.dial :air1
@22:04:37.57 Positioning Slug by 0.324ml
@22:04:37.66 AS.seek 0.326ml
@22:10:04.07 delay 5 seconds
@22:10:09.09 ATV.dial ARV
@22:10:09.92 ASV.dial :PCRlock
@22:10:10.32 delay 5 seconds
@22:10:15.34 Running AARPP4 Profile58CIQ2 thermocycling
@22:10:15.36 <PCR> PCR.program Profile58CIQ2
@22:10:15.47 Profile58CIQ2 should complete in < 1:36:15
@22:10:15.48 PCR.run 2:05:18.75
@23:31:22.97 PCR.off
@23:31:23.52 <MFBPCR> Running AARPP4 MeltProfile58 thermocycling
@23:31:23.54 <PCR> PCR.program MeltProfile58
@23:31:23.64 MeltProfile58 should complete in < 12:33
@23:31:23.65 PCR.run 20:41.25
@23:39:05.58 PCR.off
@23:39:06.57 <MFBPCR> PCR for AARPP4 completed -- closed /var/log/esp/AARPP421apr2821h.pcr
@23:39:06.64 Cleaning PCR
@23:39:06.65 AFV.select :carrierSolution
@23:39:06.77 delay 2 seconds
@23:39:08.80 AS.reconfigure ASconfig
@23:39:08.85 AS.seek 0.5ml
@23:39:19.16 delay 1 second
@23:39:20.18 AFV.select :holdingCoil
@23:39:20.28 ASV.dial :waste1
@23:39:20.68 ATV.dial :air3
@23:39:22.04 AS.reconfigure ASslow1
@23:39:22.10 AS.seek 0.51ml
@23:39:24.39 delay 2 seconds
@23:39:26.41 ATV.dial :bleach
@23:39:27.08 AS.seek 0.81ml
@23:40:27.66 delay 3 seconds
@23:40:30.68 ATV.dial :air3
@23:40:31.29 delay 3 seconds
@23:40:34.36 AS.seek 0.835ml
@23:40:39.77 delay 3 seconds
@23:40:42.79 ATV.dial :bleach
@23:40:43.45 AS.seek 0.82ml
@23:40:46.76 delay 3 seconds
@23:40:49.78 ARV.dial :waste
@23:40:50.43 delay 3 seconds
@23:40:53.45 ATV.dial :ARV
@23:40:54.37 AS.reconfigure ASconfig
@23:40:54.43 AS.seek 0.47ml
@23:41:14.85 AFV.select :carrierSolution
@23:41:14.96 delay 2 seconds
@23:41:16.99 AS.seek 1ml
@23:41:47.73 delay 1 second
@23:41:48.75 AFV.select :holdingCoil
@23:41:48.85 delay 1 second
@23:41:49.88 AS.reconfigure ASfast
@23:41:49.93 AS.seek 0ml
@23:42:17.27 AFV.select :carrierSolution
@23:42:17.38 delay 2 seconds
@23:42:19.40 AS.reconfigure ASconfig
@23:42:19.46 AS.seek 1ml
@23:43:17.20 delay 1 second
@23:43:18.22 AFV.select :holdingCoil
@23:43:18.32 delay 1 second
@23:43:19.35 AS.reconfigure ASfast
@23:43:19.40 AS.seek 0ml
@23:43:46.74 AFV.select :carrierSolution
@23:43:46.85 delay 2 seconds
@23:43:48.87 AS.reconfigure ASconfig
@23:43:48.93 AS.seek 1ml
@23:44:46.71 delay 1 second
@23:44:47.73 AFV.select :holdingCoil
@23:44:47.83 delay 1 second
@23:44:48.86 AS.reconfigure ASfast
@23:44:48.91 AS.seek 0ml
@23:45:16.24 ARV.dial between carrier and air1
@23:45:16.76 ATV.dial :air3
@23:45:18.11 AS.reconfigure ASslow1
@23:45:18.17 AS.seek 0.01ml
@23:45:20.62 delay 2 seconds
@23:45:22.64 ATV.dial :bleach
@23:45:23.28 AS.seek 0.81ml
@23:48:04.43 delay 3 seconds
@23:48:07.45 ATV.dial :air3
@23:48:08.04 delay 3 seconds
@23:48:11.11 AS.seek 0.835ml
@23:48:16.51 delay 3 seconds
@23:48:19.53 ATV.dial :bleach
@23:48:20.17 AS.seek 0.82ml
@23:48:23.56 delay 3 seconds
@23:48:26.58 ATV.dial PCR
@23:48:27.01 AS.reconfigure ASconfig
@23:48:27.07 AS.seek 0ml
@23:49:14.51 PCR.program :bleachProfile
@23:49:14.58 PCR program should complete in < 2:45
@23:49:14.59 PCR.run 10:00
@23:52:10.77 PCR.off
@23:52:10.82 AFV.select :carrierSolution
@23:52:10.93 delay 2 seconds
@23:52:12.96 AS.seek 1ml
@23:53:10.74 delay 1 second
@23:53:11.76 AFV.select :holdingCoil
@23:53:11.86 delay 1 second
@23:53:12.89 AS.reconfigure ASfast
@23:53:12.94 AS.seek 0ml
@23:53:40.29 delay 3 seconds
@23:53:43.31 AFV.select :carrierSolution
@23:53:43.41 delay 2 seconds
@23:53:45.44 AS.reconfigure ASconfig
@23:53:45.50 AS.seek 1ml
@23:54:43.27 delay 1 second
@23:54:44.29 AFV.select :holdingCoil
@23:54:44.39 delay 1 second
@23:54:45.42 AS.reconfigure ASfast
@23:54:45.47 AS.seek 0ml
@23:55:12.79 delay 3 seconds
@23:55:15.81 AFV.select :carrierSolution
@23:55:15.91 delay 2 seconds
@23:55:17.94 AS.reconfigure ASconfig
@23:55:18.00 AS.seek 1ml
@23:56:15.74 delay 1 second
@23:56:16.76 AFV.select :holdingCoil
@23:56:16.86 delay 1 second
@23:56:17.89 AS.reconfigure ASfast
@23:56:17.94 AS.seek 0ml
@23:56:45.29 delay 3 seconds
@23:56:48.31 AFV.select :carrierSolution
@23:56:48.41 delay 2 seconds
@23:56:50.43 AS.reconfigure ASconfig
@23:56:50.50 AS.seek 1ml
@23:57:48.29 delay 1 second
@23:57:49.31 AFV.select :holdingCoil
@23:57:49.41 delay 1 second
@23:57:50.44 AS.reconfigure ASfast
@23:57:50.49 AS.seek 0ml
@23:58:17.84 delay 3 seconds
@23:58:20.86 AFV.select :carrierSolution
@23:58:20.96 delay 2 seconds
@23:58:22.98 AS.reconfigure ASconfig
@23:58:23.05 AS.seek 1ml
@23:59:20.79 delay 1 second
@23:59:21.81 AFV.select :holdingCoil
@23:59:21.91 delay 1 second
@23:59:22.94 AS.reconfigure ASnorm
@23:59:22.99 AS.seek 0ml
@00:00:13.73CEST29-Apr-21 <MAIN> AFV.select :carrierSolution
@00:00:13.84 delay 2 seconds
@00:00:15.87 AS.reconfigure ASconfig
@00:00:15.92 AS.seek 0.5ml
@00:00:44.96 delay 1 second
@00:00:45.98 AFV.select :holdingCoil
@00:00:46.08 ATV.dial :air1
@00:00:47.65 AS.reconfigure ASslow2
@00:00:47.71 AS.seek 0.506ml
@00:00:54.23 ATV.dial :extSrc
@00:00:56.01 AS.seek 0.512ml
@00:01:02.16 delay 5 seconds
@00:01:07.18 ATV.dial :air1
@00:01:08.98 AS.reconfigure ASslow1
@00:01:09.04 AS.seek 0.542ml
@00:01:15.35 ATV.dial :waste
@00:01:15.92 AS.reconfigure ASconfig
@00:01:15.98 AS.seek 0ml
@00:01:47.59 <MFBPCR> Setting up PCR for AARPP4 -- writing /var/log/esp/AARPP421apr2900h.pcr
@00:01:47.62 Building PCR Slug from extSrc with MM2 mix, PP4 primer
@00:01:47.64 Priming with carrier
@00:01:47.66 ATV.dial ARV
@00:01:49.26 ARV.dial :carrier
@00:01:49.62 AS.reconfigure ASnorm
@00:01:49.68 AS.seek 0.5ml
@00:02:15.24 AFV.select :carrierSolution
@00:02:15.35 delay 2 seconds
@00:02:17.38 AS.reconfigure ASconfig
@00:02:17.45 AS.seek 1ml
@00:02:46.48 delay 1 second
@00:02:47.51 AFV.select :holdingCoil
@00:02:47.61 delay 1 second
@00:02:48.64 ATV.dial :waste
@00:02:50.46 AS.reconfigure ASnorm
@00:02:50.53 AS.seek 0ml
@00:03:41.23 AFV.select :carrierSolution
@00:03:41.34 delay 2 seconds
@00:03:43.73 AS.reconfigure ASconfig
@00:03:43.80 AS.seek 0.5ml
@00:04:12.82 delay 1 second
@00:04:13.84 AFV.select :holdingCoil
@00:04:13.94 Building Slug
@00:04:13.95 ATV.dial :ARV
@00:04:15.73 delay 2 seconds
@00:04:17.75 ARV.dial :air1
@00:04:18.18 delay 2 seconds
@00:04:20.25 AS.reconfigure ASslow2
@00:04:20.31 AS.seek 0.506ml
@00:04:26.66 delay 2 seconds
@00:04:28.68 ARV.dial :MM2
@00:04:30.27 delay 2 seconds
@00:04:32.29 Adding MM2
@00:04:32.37 AS.seek 0.524ml
@00:04:50.98 delay 25 seconds
@00:05:16.00 ARV.dial :PP4
@00:05:16.42 Adding PP4
@00:05:16.43 delay 2 seconds
@00:05:18.51 AS.seek 0.53ml
@00:05:24.93 delay 10 seconds
@00:05:34.96 ARV.dial :air3
@00:05:35.38 adding ARV air
@00:05:35.39 delay 2 seconds
@00:05:37.47 AS.seek 0.536ml
@00:05:44.07 delay 5 seconds
@00:05:49.09 ARV.dial :carrier
@00:05:50.42 delay 7 seconds
@00:05:57.50 AS.seek 0.594ml
@00:06:56.13 delay 5 seconds
@00:07:01.15 ATV.dial :extSrc
@00:07:01.76 delay 2 seconds
@00:07:03.83 AS.seek 0.6ml
@00:07:10.20 delay 4 seconds
@00:07:14.22 ATV.dial ARV
@00:07:14.81 delay 2 seconds
@00:07:16.83 ARV.dial :carrier
@00:07:16.84 delay 2 seconds
@00:07:18.92 AS.seek 0.65ml
@00:08:10.27 delay 4 seconds
@00:08:14.29 ATV.dial PCR
@00:08:15.10 ASV.dial :waste1
@00:08:15.11 ARV.dial :air1
@00:08:15.52 Positioning Slug by 0.324ml
@00:08:15.61 AS.seek 0.326ml
@00:13:42.13 delay 5 seconds
@00:13:47.15 ATV.dial ARV
@00:13:47.98 ASV.dial :PCRlock
@00:13:48.38 delay 5 seconds
@00:13:53.40 Running AARPP4 Profile58CIQ2 thermocycling
@00:13:53.41 <PCR> PCR.program Profile58CIQ2
@00:13:53.53 Profile58CIQ2 should complete in < 1:36:15
@00:13:53.54 PCR.run 2:05:18.75
@01:34:49.39 PCR.off
@01:34:49.96 <MFBPCR> Running AARPP4 MeltProfile58 thermocycling
@01:34:49.97 <PCR> PCR.program MeltProfile58
@01:34:50.07 MeltProfile58 should complete in < 12:33
@01:34:50.08 PCR.run 20:41.25
@01:42:34.75 PCR.off
@01:42:35.73 <MFBPCR> PCR for AARPP4 completed -- closed /var/log/esp/AARPP421apr2900h.pcr
@01:42:35.80 Cleaning PCR
@01:42:35.81 AFV.select :carrierSolution
@01:42:35.93 delay 2 seconds
@01:42:37.96 AS.reconfigure ASconfig
@01:42:38.01 AS.seek 0.5ml
@01:42:48.34 delay 1 second
@01:42:49.36 AFV.select :holdingCoil
@01:42:49.46 ASV.dial :waste1
@01:42:49.87 ATV.dial :air3
@01:42:51.23 AS.reconfigure ASslow1
@01:42:51.29 AS.seek 0.51ml
@01:42:53.68 delay 2 seconds
@01:42:55.70 ATV.dial :bleach
@01:42:56.36 AS.seek 0.81ml
@01:43:56.99 delay 3 seconds
@01:44:00.01 ATV.dial :air3
@01:44:00.62 delay 3 seconds
@01:44:03.69 AS.seek 0.835ml
@01:44:09.10 delay 3 seconds
@01:44:12.12 ATV.dial :bleach
@01:44:12.76 AS.seek 0.82ml
@01:44:16.07 delay 3 seconds
@01:44:19.09 ARV.dial :waste
@01:44:19.73 delay 3 seconds
@01:44:22.75 ATV.dial :ARV
@01:44:23.68 AS.reconfigure ASconfig
@01:44:23.74 AS.seek 0.47ml
@01:44:44.13 AFV.select :carrierSolution
@01:44:44.24 delay 2 seconds
@01:44:46.27 AS.seek 1ml
@01:45:17.01 delay 1 second
@01:45:18.03 AFV.select :holdingCoil
@01:45:18.13 delay 1 second
@01:45:19.52 AS.reconfigure ASfast
@01:45:19.59 AS.seek 0ml
@01:45:46.92 AFV.select :carrierSolution
@01:45:47.03 delay 2 seconds
@01:45:49.06 AS.reconfigure ASconfig
@01:45:49.11 AS.seek 1ml
@01:46:46.90 delay 1 second
@01:46:47.92 AFV.select :holdingCoil
@01:46:48.02 delay 1 second
@01:46:49.05 AS.reconfigure ASfast
@01:46:49.10 AS.seek 0ml
@01:47:16.43 AFV.select :carrierSolution
@01:47:16.54 delay 2 seconds
@01:47:18.57 AS.reconfigure ASconfig
@01:47:18.62 AS.seek 1ml
@01:48:16.35 delay 1 second
@01:48:17.37 AFV.select :holdingCoil
@01:48:17.47 delay 1 second
@01:48:18.50 AS.reconfigure ASfast
@01:48:18.55 AS.seek 0ml
@01:48:45.88 ARV.dial between carrier and air1
@01:48:46.42 ATV.dial :air3
@01:48:47.78 AS.reconfigure ASslow1
@01:48:47.85 AS.seek 0.01ml
@01:48:50.12 delay 2 seconds
@01:48:52.14 ATV.dial :bleach
@01:48:52.80 AS.seek 0.81ml
@01:51:33.95 delay 3 seconds
@01:51:36.97 ATV.dial :air3
@01:51:37.56 delay 3 seconds
@01:51:40.63 AS.seek 0.835ml
@01:51:46.00 delay 3 seconds
@01:51:49.02 ATV.dial :bleach
@01:51:49.68 AS.seek 0.82ml
@01:51:52.99 delay 3 seconds
@01:51:56.01 ATV.dial PCR
@01:51:56.45 AS.reconfigure ASconfig
@01:51:56.51 AS.seek 0ml
@01:52:43.93 PCR.program :bleachProfile
@01:52:43.99 PCR program should complete in < 2:45
@01:52:44.00 PCR.run 10:00
@01:55:39.80 PCR.off
@01:55:39.85 AFV.select :carrierSolution
@01:55:39.96 delay 2 seconds
@01:55:41.99 AS.seek 1ml
@01:56:39.73 delay 1 second
@01:56:40.75 AFV.select :holdingCoil
@01:56:40.85 delay 1 second
@01:56:41.88 AS.reconfigure ASfast
@01:56:41.93 AS.seek 0ml
@01:57:09.27 delay 3 seconds
@01:57:12.29 AFV.select :carrierSolution
@01:57:12.39 delay 2 seconds
@01:57:14.41 AS.reconfigure ASconfig
@01:57:14.48 AS.seek 1ml
@01:58:12.27 delay 1 second
@01:58:13.29 AFV.select :holdingCoil
@01:58:13.39 delay 1 second
@01:58:14.42 AS.reconfigure ASfast
@01:58:14.47 AS.seek 0ml
@01:58:41.82 delay 3 seconds
@01:58:44.84 AFV.select :carrierSolution
@01:58:44.94 delay 2 seconds
@01:58:46.97 AS.reconfigure ASconfig
@01:58:47.41 AS.seek 1ml
@01:59:45.15 delay 1 second
@01:59:46.17 AFV.select :holdingCoil
@01:59:46.27 delay 1 second
@01:59:47.30 AS.reconfigure ASfast
@01:59:47.35 AS.seek 0ml
@02:00:14.70 delay 3 seconds
@02:00:17.72 AFV.select :carrierSolution
@02:00:17.82 delay 2 seconds
@02:00:19.85 AS.reconfigure ASconfig
@02:00:19.91 AS.seek 1ml
@02:01:17.71 delay 1 second
@02:01:18.73 AFV.select :holdingCoil
@02:01:18.83 delay 1 second
@02:01:19.86 AS.reconfigure ASfast
@02:01:19.91 AS.seek 0ml
@02:01:47.26 delay 3 seconds
@02:01:50.28 AFV.select :carrierSolution
@02:01:50.38 delay 2 seconds
@02:01:52.41 AS.reconfigure ASconfig
@02:01:52.47 AS.seek 1ml
@02:02:50.21 delay 1 second
@02:02:51.23 AFV.select :holdingCoil
@02:02:51.33 delay 1 second
@02:02:52.36 AS.reconfigure ASnorm
@02:02:52.41 AS.seek 0ml
@02:03:43.11 <MAIN> AFV.select :carrierSolution
@02:03:43.21 delay 2 seconds
@02:03:45.24 AS.reconfigure ASconfig
@02:03:45.29 AS.seek 0.5ml
@02:04:14.31 delay 1 second
@02:04:15.33 AFV.select :holdingCoil
@02:04:15.43 ATV.dial :air1
@02:04:17.03 AS.reconfigure ASslow1
@02:04:17.09 AS.seek 0.52ml
@02:04:21.40 delay 5 seconds
@02:04:26.42 ATV.dial :extSrc
@02:04:28.23 AS.reconfigure ASslow2
@02:04:28.29 AS.seek 0.538ml
@02:04:46.68 delay 10 seconds
@02:04:56.70 ATV.dial :air1
@02:04:58.51 AS.reconfigure ASslow1
@02:04:58.57 AS.seek 0.568ml
@02:05:04.98 ATV.dial :waste
@02:05:05.57 AS.reconfigure ASconfig
@02:05:05.63 AS.seek 0ml
@02:05:38.71 <MFBPCR> Setting up PCR for AARPP4 -- writing /var/log/esp/AARPP421apr2902h.pcr
@02:05:38.74 Building PCR Slug from extSrc with MM2 mix, PP4 primer
@02:05:38.76 Priming with carrier
@02:05:38.78 ATV.dial ARV
@02:05:40.38 ARV.dial :carrier
@02:05:40.73 AS.reconfigure ASnorm
@02:05:40.79 AS.seek 0.5ml
@02:06:06.37 AFV.select :carrierSolution
@02:06:06.49 delay 2 seconds
@02:06:08.52 AS.reconfigure ASconfig
@02:06:08.57 AS.seek 1ml
@02:06:37.59 delay 1 second
@02:06:38.61 AFV.select :holdingCoil
@02:06:38.71 delay 1 second
@02:06:39.73 ATV.dial :waste
@02:06:41.51 AS.reconfigure ASnorm
@02:06:41.57 AS.seek 0ml
@02:07:32.29 AFV.select :carrierSolution
@02:07:32.40 delay 2 seconds
@02:07:34.43 AS.reconfigure ASconfig
@02:07:34.48 AS.seek 0.5ml
@02:08:03.52 delay 1 second
@02:08:04.54 AFV.select :holdingCoil
@02:08:04.64 Building Slug
@02:08:04.66 ATV.dial :ARV
@02:08:06.43 delay 2 seconds
@02:08:08.45 ARV.dial :air1
@02:08:08.86 delay 2 seconds
@02:08:10.93 AS.reconfigure ASslow2
@02:08:10.99 AS.seek 0.506ml
@02:08:17.62 delay 2 seconds
@02:08:19.64 ARV.dial :MM2
@02:08:21.23 delay 2 seconds
@02:08:23.25 Adding MM2
@02:08:23.34 AS.seek 0.524ml
@02:08:41.98 delay 25 seconds
@02:09:07.00 ARV.dial :PP4
@02:09:07.42 Adding PP4
@02:09:07.43 delay 2 seconds
@02:09:09.51 AS.seek 0.53ml
@02:09:15.98 delay 10 seconds
@02:09:26.01 ARV.dial :air3
@02:09:26.43 adding ARV air
@02:09:26.44 delay 2 seconds
@02:09:28.52 AS.seek 0.536ml
@02:09:35.13 delay 5 seconds
@02:09:40.15 ARV.dial :carrier
@02:09:41.49 delay 7 seconds
@02:09:48.57 AS.seek 0.594ml
@02:10:47.46 delay 5 seconds
@02:10:52.48 ATV.dial :extSrc
@02:10:53.10 delay 2 seconds
@02:10:55.17 AS.seek 0.6ml
@02:11:01.59 delay 4 seconds
@02:11:05.61 ATV.dial ARV
@02:11:06.20 delay 2 seconds
@02:11:08.22 ARV.dial :carrier
@02:11:08.23 delay 2 seconds
@02:11:10.31 AS.seek 0.65ml
@02:12:01.02 delay 4 seconds
@02:12:05.04 ATV.dial PCR
@02:12:05.87 ASV.dial :waste1
@02:12:05.88 ARV.dial :air1
@02:12:06.30 Positioning Slug by 0.324ml
@02:12:06.39 AS.seek 0.326ml
@02:17:32.43 delay 5 seconds
@02:17:37.45 ATV.dial ARV
@02:17:38.28 ASV.dial :PCRlock
@02:17:38.68 delay 5 seconds
@02:17:43.70 Running AARPP4 Profile58CIQ2 thermocycling
@02:17:43.72 <PCR> PCR.program Profile58CIQ2
@02:17:44.24 Profile58CIQ2 should complete in < 1:36:15
@02:17:44.25 PCR.run 2:05:18.75
@03:38:31.13 PCR.off
@03:38:31.69 <MFBPCR> Running AARPP4 MeltProfile58 thermocycling
@03:38:31.70 <PCR> PCR.program MeltProfile58
@03:38:31.80 MeltProfile58 should complete in < 12:33
@03:38:31.81 PCR.run 20:41.25
@03:46:13.50 PCR.off
@03:46:14.48 <MFBPCR> PCR for AARPP4 completed -- closed /var/log/esp/AARPP421apr2902h.pcr
@03:46:14.55 Cleaning PCR
@03:46:14.56 AFV.select :carrierSolution
@03:46:14.68 delay 2 seconds
@03:46:16.71 AS.reconfigure ASconfig
@03:46:16.76 AS.seek 0.5ml
@03:46:27.06 delay 1 second
@03:46:28.08 AFV.select :holdingCoil
@03:46:28.18 ASV.dial :waste1
@03:46:28.57 ATV.dial :air3
@03:46:29.94 AS.reconfigure ASslow1
@03:46:30.00 AS.seek 0.51ml
@03:46:32.29 delay 2 seconds
@03:46:34.31 ATV.dial :bleach
@03:46:34.98 AS.seek 0.81ml
@03:47:35.60 delay 3 seconds
@03:47:38.62 ATV.dial :air3
@03:47:39.21 delay 3 seconds
@03:47:42.28 AS.seek 0.835ml
@03:47:47.57 delay 3 seconds
@03:47:50.59 ATV.dial :bleach
@03:47:51.23 AS.seek 0.82ml
@03:47:54.71 delay 3 seconds
@03:47:57.73 ARV.dial :waste
@03:47:58.37 delay 3 seconds
@03:48:01.39 ATV.dial :ARV
@03:48:02.33 AS.reconfigure ASconfig
@03:48:02.39 AS.seek 0.47ml
@03:48:22.81 AFV.select :carrierSolution
@03:48:22.92 delay 2 seconds
@03:48:24.95 AS.seek 1ml
@03:48:55.68 delay 1 second
@03:48:56.70 AFV.select :holdingCoil
@03:48:56.80 delay 1 second
@03:48:57.83 AS.reconfigure ASfast
@03:48:57.88 AS.seek 0ml
@03:49:25.21 AFV.select :carrierSolution
@03:49:25.32 delay 2 seconds
@03:49:27.34 AS.reconfigure ASconfig
@03:49:27.40 AS.seek 1ml
@03:50:25.18 delay 1 second
@03:50:26.20 AFV.select :holdingCoil
@03:50:26.30 delay 1 second
@03:50:27.33 AS.reconfigure ASfast
@03:50:27.38 AS.seek 0ml
@03:50:54.71 AFV.select :carrierSolution
@03:50:54.81 delay 2 seconds
@03:50:56.84 AS.reconfigure ASconfig
@03:50:56.89 AS.seek 1ml
@03:51:54.64 delay 1 second
@03:51:55.66 AFV.select :holdingCoil
@03:51:55.76 delay 1 second
@03:51:56.79 AS.reconfigure ASfast
@03:51:56.84 AS.seek 0ml
@03:52:24.17 ARV.dial between carrier and air1
@03:52:24.70 ATV.dial :air3
@03:52:26.07 AS.reconfigure ASslow1
@03:52:26.12 AS.seek 0.01ml
@03:52:28.65 delay 2 seconds
@03:52:30.67 ATV.dial :bleach
@03:52:31.31 AS.seek 0.81ml
@03:55:12.43 delay 3 seconds
@03:55:15.45 ATV.dial :air3
@03:55:16.04 delay 3 seconds
@03:55:19.11 AS.seek 0.835ml
@03:55:24.47 delay 3 seconds
@03:55:27.49 ATV.dial :bleach
@03:55:28.12 AS.seek 0.82ml
@03:55:31.42 delay 3 seconds
@03:55:34.44 ATV.dial PCR
@03:55:34.87 AS.reconfigure ASconfig
@03:55:34.93 AS.seek 0ml
@03:56:22.37 PCR.program :bleachProfile
@03:56:22.44 PCR program should complete in < 2:45
@03:56:22.45 PCR.run 10:00
@03:59:18.65 PCR.off
@03:59:18.69 AFV.select :carrierSolution
@03:59:18.80 delay 2 seconds
@03:59:20.83 AS.seek 1ml
@04:00:18.62 delay 1 second
@04:00:19.64 AFV.select :holdingCoil
@04:00:19.74 delay 1 second
@04:00:20.77 AS.reconfigure ASfast
@04:00:20.82 AS.seek 0ml
@04:00:48.17 delay 3 seconds
@04:00:51.19 AFV.select :carrierSolution
@04:00:51.29 delay 2 seconds
@04:00:53.32 AS.reconfigure ASconfig
@04:00:53.38 AS.seek 1ml
@04:01:51.15 delay 1 second
@04:01:52.17 AFV.select :holdingCoil
@04:01:52.27 delay 1 second
@04:01:53.30 AS.reconfigure ASfast
@04:01:53.35 AS.seek 0ml
@04:02:20.70 delay 3 seconds
@04:02:23.72 AFV.select :carrierSolution
@04:02:23.82 delay 2 seconds
@04:02:25.85 AS.reconfigure ASconfig
@04:02:25.91 AS.seek 1ml
@04:03:23.65 delay 1 second
@04:03:24.67 AFV.select :holdingCoil
@04:03:24.77 delay 1 second
@04:03:25.80 AS.reconfigure ASfast
@04:03:25.85 AS.seek 0ml
@04:03:53.18 delay 3 seconds
@04:03:56.20 AFV.select :carrierSolution
@04:03:56.30 delay 2 seconds
@04:03:58.33 AS.reconfigure ASconfig
@04:03:58.39 AS.seek 1ml
@04:04:56.15 delay 1 second
@04:04:57.17 AFV.select :holdingCoil
@04:04:57.27 delay 1 second
@04:04:58.30 AS.reconfigure ASfast
@04:04:58.35 AS.seek 0ml
@04:05:25.70 delay 3 seconds
@04:05:28.72 AFV.select :carrierSolution
@04:05:28.82 delay 2 seconds
@04:05:30.84 AS.reconfigure ASconfig
@04:05:30.91 AS.seek 1ml
@04:06:28.67 delay 1 second
@04:06:29.69 AFV.select :holdingCoil
@04:06:29.79 delay 1 second
@04:06:30.82 AS.reconfigure ASnorm
@04:06:30.87 AS.seek 0ml
@04:07:21.58 <MAIN> AFV.select :carrierSolution
@04:07:21.69 delay 2 seconds
@04:07:23.72 AS.reconfigure ASconfig
@04:07:23.77 AS.seek 0.5ml
@04:07:52.81 delay 1 second
@04:07:53.83 AFV.select :holdingCoil
@04:07:53.93 ATV.dial :air1
@04:07:55.53 AS.reconfigure ASslow2
@04:07:55.59 AS.seek 0.506ml
@04:08:01.87 ATV.dial :extSrc
@04:08:03.69 AS.seek 0.512ml
@04:08:09.85 delay 5 seconds
@04:08:14.87 ATV.dial :air1
@04:08:16.69 AS.reconfigure ASslow1
@04:08:16.75 AS.seek 0.542ml
@04:08:23.04 ATV.dial :waste
@04:08:23.62 AS.reconfigure ASconfig
@04:08:23.68 AS.seek 0ml
@04:08:55.29 <MFBPCR> Setting up PCR for AARPP4 -- writing /var/log/esp/AARPP421apr2904h.pcr
@04:08:55.31 Building PCR Slug from extSrc with MM2 mix, PP4 primer
@04:08:55.33 Priming with carrier
@04:08:55.35 ATV.dial ARV
@04:08:56.95 ARV.dial :carrier
@04:08:57.30 AS.reconfigure ASnorm
@04:08:57.36 AS.seek 0.5ml
@04:09:22.99 AFV.select :carrierSolution
@04:09:23.11 delay 2 seconds
@04:09:25.14 AS.reconfigure ASconfig
@04:09:25.19 AS.seek 1ml
@04:09:54.20 delay 1 second
@04:09:55.22 AFV.select :holdingCoil
@04:09:55.32 delay 1 second
@04:09:56.34 ATV.dial :waste
@04:09:58.12 AS.reconfigure ASnorm
@04:09:58.18 AS.seek 0ml
@04:10:48.87 AFV.select :carrierSolution
@04:10:48.98 delay 2 seconds
@04:10:51.01 AS.reconfigure ASconfig
@04:10:51.43 AS.seek 0.5ml
@04:11:20.51 delay 1 second
@04:11:21.53 AFV.select :holdingCoil
@04:11:21.63 Building Slug
@04:11:21.64 ATV.dial :ARV
@04:11:23.40 delay 2 seconds
@04:11:25.42 ARV.dial :air1
@04:11:25.83 delay 2 seconds
@04:11:27.90 AS.reconfigure ASslow2
@04:11:27.96 AS.seek 0.506ml
@04:11:34.20 delay 2 seconds
@04:11:36.22 ARV.dial :MM2
@04:11:37.81 delay 2 seconds
@04:11:39.83 Adding MM2
@04:11:39.91 AS.seek 0.524ml
@04:11:58.51 delay 25 seconds
@04:12:23.53 ARV.dial :PP4
@04:12:23.95 Adding PP4
@04:12:23.96 delay 2 seconds
@04:12:26.04 AS.seek 0.53ml
@04:12:32.60 delay 10 seconds
@04:12:42.63 ARV.dial :air3
@04:12:43.06 adding ARV air
@04:12:43.07 delay 2 seconds
@04:12:45.15 AS.seek 0.536ml
@04:12:51.32 delay 5 seconds
@04:12:56.34 ARV.dial :carrier
@04:12:57.68 delay 7 seconds
@04:13:04.76 AS.seek 0.594ml
@04:14:03.35 delay 5 seconds
@04:14:08.37 ATV.dial :extSrc
@04:14:08.98 delay 2 seconds
@04:14:11.05 AS.seek 0.6ml
@04:14:17.35 delay 4 seconds
@04:14:21.37 ATV.dial ARV
@04:14:21.96 delay 2 seconds
@04:14:23.98 ARV.dial :carrier
@04:14:23.99 delay 2 seconds
@04:14:26.07 AS.seek 0.65ml
@04:15:16.71 delay 4 seconds
@04:15:20.73 ATV.dial PCR
@04:15:21.56 ASV.dial :waste1
@04:15:21.57 ARV.dial :air1
@04:15:21.99 Positioning Slug by 0.324ml
@04:15:22.08 AS.seek 0.326ml
@04:20:48.37 delay 5 seconds
@04:20:53.39 ATV.dial ARV
@04:20:54.21 ASV.dial :PCRlock
@04:20:54.62 delay 5 seconds
@04:20:59.64 Running AARPP4 Profile58CIQ2 thermocycling
@04:20:59.66 <PCR> PCR.program Profile58CIQ2
@04:20:59.77 Profile58CIQ2 should complete in < 1:36:15
@04:20:59.78 PCR.run 2:05:18.75
@05:41:46.65 PCR.off
@05:41:47.22 <MFBPCR> Running AARPP4 MeltProfile58 thermocycling
@05:41:47.23 <PCR> PCR.program MeltProfile58
@05:41:47.33 MeltProfile58 should complete in < 12:33
@05:41:47.34 PCR.run 20:41.25
@05:49:31.93 PCR.off
@05:49:32.90 <MFBPCR> PCR for AARPP4 completed -- closed /var/log/esp/AARPP421apr2904h.pcr
@05:49:32.98 Cleaning PCR
@05:49:32.99 AFV.select :carrierSolution
@05:49:33.10 delay 2 seconds
@05:49:35.13 AS.reconfigure ASconfig
@05:49:35.18 AS.seek 0.5ml
@05:49:45.51 delay 1 second
@05:49:46.53 AFV.select :holdingCoil
@05:49:46.63 ASV.dial :waste1
@05:49:47.03 ATV.dial :air3
@05:49:48.37 AS.reconfigure ASslow1
@05:49:48.43 AS.seek 0.51ml
@05:49:50.71 delay 2 seconds
@05:49:52.73 ATV.dial :bleach
@05:49:53.37 AS.seek 0.81ml
@05:50:53.93 delay 3 seconds
@05:50:56.95 ATV.dial :air3
@05:50:57.54 delay 3 seconds
@05:51:00.61 AS.seek 0.835ml
@05:51:06.02 delay 3 seconds
@05:51:09.04 ATV.dial :bleach
@05:51:09.67 AS.seek 0.82ml
@05:51:12.96 delay 3 seconds
@05:51:15.98 ARV.dial :waste
@05:51:16.62 delay 3 seconds
@05:51:19.64 ATV.dial :ARV
@05:51:20.58 AS.reconfigure ASconfig
@05:51:20.64 AS.seek 0.47ml
@05:51:41.04 AFV.select :carrierSolution
@05:51:41.15 delay 2 seconds
@05:51:43.18 AS.seek 1ml
@05:52:13.95 delay 1 second
@05:52:14.97 AFV.select :holdingCoil
@05:52:15.07 delay 1 second
@05:52:16.10 AS.reconfigure ASfast
@05:52:16.15 AS.seek 0ml
@05:52:43.50 AFV.select :carrierSolution
@05:52:43.99 delay 2 seconds
@05:52:46.02 AS.reconfigure ASconfig
@05:52:46.07 AS.seek 1ml
@05:53:43.79 delay 1 second
@05:53:44.81 AFV.select :holdingCoil
@05:53:44.91 delay 1 second
@05:53:45.94 AS.reconfigure ASfast
@05:53:45.99 AS.seek 0ml
@05:54:13.34 AFV.select :carrierSolution
@05:54:13.45 delay 2 seconds
@05:54:15.48 AS.reconfigure ASconfig
@05:54:15.53 AS.seek 1ml
@05:55:13.37 delay 1 second
@05:55:14.39 AFV.select :holdingCoil
@05:55:14.49 delay 1 second
@05:55:15.52 AS.reconfigure ASfast
@05:55:15.57 AS.seek 0ml
@05:55:42.90 ARV.dial between carrier and air1
@05:55:43.42 ATV.dial :air3
@05:55:44.79 AS.reconfigure ASslow1
@05:55:44.85 AS.seek 0.01ml
@05:55:47.26 delay 2 seconds
@05:55:49.28 ATV.dial :bleach
@05:55:49.95 AS.seek 0.81ml
@05:58:31.17 delay 3 seconds
@05:58:34.19 ATV.dial :air3
@05:58:34.79 delay 3 seconds
@05:58:37.86 AS.seek 0.835ml
@05:58:43.15 delay 3 seconds
@05:58:46.17 ATV.dial :bleach
@05:58:46.82 AS.seek 0.82ml
@05:58:50.23 delay 3 seconds
@05:58:53.25 ATV.dial PCR
@05:58:53.68 AS.reconfigure ASconfig
@05:58:53.74 AS.seek 0ml
@05:59:41.15 PCR.program :bleachProfile
@05:59:41.22 PCR program should complete in < 2:45
@05:59:41.23 PCR.run 10:00
@06:02:37.01 PCR.off
@06:02:37.06 AFV.select :carrierSolution
@06:02:37.17 delay 2 seconds
@06:02:39.20 AS.seek 1ml
@06:03:36.96 delay 1 second
@06:03:37.98 AFV.select :holdingCoil
@06:03:38.08 delay 1 second
@06:03:39.11 AS.reconfigure ASfast
@06:03:39.16 AS.seek 0ml
@06:04:06.50 delay 3 seconds
@06:04:09.52 AFV.select :carrierSolution
@06:04:09.62 delay 2 seconds
@06:04:11.64 AS.reconfigure ASconfig
@06:04:11.71 AS.seek 1ml
@06:05:09.46 delay 1 second
@06:05:10.48 AFV.select :holdingCoil
@06:05:10.58 delay 1 second
@06:05:11.61 AS.reconfigure ASfast
@06:05:11.66 AS.seek 0ml
@06:05:39.00 delay 3 seconds
@06:05:42.02 AFV.select :carrierSolution
@06:05:42.12 delay 2 seconds
@06:05:44.14 AS.reconfigure ASconfig
@06:05:44.21 AS.seek 1ml
@06:06:41.95 delay 1 second
@06:06:42.97 AFV.select :holdingCoil
@06:06:43.07 delay 1 second
@06:06:44.49 AS.reconfigure ASfast
@06:06:44.54 AS.seek 0ml
@06:07:11.89 delay 3 seconds
@06:07:14.91 AFV.select :carrierSolution
@06:07:15.01 delay 2 seconds
@06:07:17.04 AS.reconfigure ASconfig
@06:07:17.10 AS.seek 1ml
@06:08:14.84 delay 1 second
@06:08:15.86 AFV.select :holdingCoil
@06:08:15.96 delay 1 second
@06:08:16.99 AS.reconfigure ASfast
@06:08:17.04 AS.seek 0ml
@06:08:44.37 delay 3 seconds
@06:08:47.39 AFV.select :carrierSolution
@06:08:47.49 delay 2 seconds
@06:08:49.52 AS.reconfigure ASconfig
@06:08:49.57 AS.seek 1ml
@06:09:47.32 delay 1 second
@06:09:48.34 AFV.select :holdingCoil
@06:09:48.44 delay 1 second
@06:09:49.47 AS.reconfigure ASnorm
@06:09:49.52 AS.seek 0ml
@06:10:40.25 <MAIN> deconEluateRecoveryLine extSrc
@06:10:40.26 ATV.dial :waste
@06:10:41.57 AS.seek 0ml
@06:10:41.65 ATV.dial :air3
@06:10:42.53 AS.reconfigure ASslow1
@06:10:42.58 AS.seek 0.01ml
@06:10:45.06 delay 2 seconds
@06:10:47.08 ATV.dial :bleach
@06:10:47.56 AS.seek 0.31ml
@06:11:48.15 delay 3 seconds
@06:11:51.17 ATV.dial :air3
@06:11:51.76 delay 3 seconds
@06:11:54.83 AS.seek 0.335ml
@06:12:00.16 delay 3 seconds
@06:12:03.18 ATV.dial :bleach
@06:12:03.82 AS.seek 0.32ml
@06:12:07.06 delay 3 seconds
@06:12:10.08 ATV.dial :extSrc
@06:12:10.73 AS.seek 0ml
@06:13:15.37 AFV.select :carrierSolution
@06:13:15.48 delay 2 seconds
@06:13:17.51 AS.reconfigure ASconfig
@06:13:17.56 AS.seek 0.5ml
@06:13:46.61 delay 1 second
@06:13:47.63 AFV.select :holdingCoil
@06:13:47.73 ATV.dial :extSrc
@06:13:47.75 AS.reconfigure ASslow1
@06:13:47.82 AS.seek 0ml
@06:15:28.81 AFV.select :carrierSolution
@06:15:28.92 delay 2 seconds
@06:15:30.95 AS.reconfigure ASconfig
@06:15:31.00 AS.seek 0.5ml
@06:16:00.01 delay 1 second
@06:16:01.03 AFV.select :holdingCoil
@06:16:01.13 ATV.dial :extSrc
@06:16:01.15 AS.reconfigure ASslow1
@06:16:01.22 AS.seek 0ml
@06:17:42.04 AFV.select :carrierSolution
@06:17:42.15 delay 2 seconds
@06:17:44.18 AS.reconfigure ASconfig
@06:17:44.23 AS.seek 0.5ml
@06:18:13.26 delay 1 second
@06:18:14.28 AFV.select :holdingCoil
@06:18:14.38 ATV.dial :extSrc
@06:18:14.40 AS.reconfigure ASslow1
@06:18:14.46 AS.seek 0ml
@06:19:55.43 AFV.select :carrierSolution
@06:19:55.54 delay 2 seconds
@06:19:57.57 AS.reconfigure ASconfig
@06:19:57.62 AS.seek 0.5ml
@06:20:26.68 delay 1 second
@06:20:27.70 AFV.select :holdingCoil
@06:20:27.80 ATV.dial :extSrc
@06:20:27.82 AS.reconfigure ASslow1
@06:20:27.88 AS.seek 0ml
@06:22:08.73 ATV.dial :air2
@06:22:10.14 AS.reconfigure ASslow0
@06:22:10.20 AS.seek 0.5ml
@06:23:00.81 ATV.dial :extSrc
@06:23:02.38 AS.seek 0.02ml
@06:23:50.98 delay 1 second
@06:23:52.00 ATV.dial :waste
@06:23:53.37 AS.reconfigure ASconfig
@06:23:53.43 AS.seek 0ml
@06:23:54.87 deconEluateRecoveryLine extSrc
@06:23:54.88 ATV.dial :waste
@06:23:54.91 AS.reconfigure ASnorm
@06:23:54.97 AS.seek 0ml
@06:23:55.11 ATV.dial :air3
@06:23:56.01 AS.reconfigure ASslow1
@06:23:56.07 AS.seek 0.01ml
@06:23:58.67 delay 2 seconds
@06:24:00.69 ATV.dial :bleach
@06:24:01.19 AS.seek 0.31ml
@06:25:01.86 delay 3 seconds
@06:25:04.88 ATV.dial :air3
@06:25:05.48 delay 3 seconds
@06:25:08.55 AS.seek 0.335ml
@06:25:13.88 delay 3 seconds
@06:25:16.90 ATV.dial :bleach
@06:25:17.53 AS.seek 0.32ml
@06:25:20.95 delay 3 seconds
@06:25:23.97 ATV.dial :extSrc
@06:25:24.64 AS.seek 0ml
@06:26:29.39 AFV.select :carrierSolution
@06:26:29.50 delay 2 seconds
@06:26:31.53 AS.reconfigure ASconfig
@06:26:31.58 AS.seek 0.5ml
@06:27:00.61 delay 1 second
@06:27:01.63 AFV.select :holdingCoil
@06:27:01.73 ATV.dial :extSrc
@06:27:01.75 AS.reconfigure ASslow1
@06:27:01.81 AS.seek 0ml
@06:28:42.75 AFV.select :carrierSolution
@06:28:42.86 delay 2 seconds
@06:28:44.89 AS.reconfigure ASconfig
@06:28:44.94 AS.seek 0.5ml
@06:29:13.97 delay 1 second
@06:29:14.99 AFV.select :holdingCoil
@06:29:15.09 ATV.dial :extSrc
@06:29:15.11 AS.reconfigure ASslow1
@06:29:15.17 AS.seek 0ml
@06:30:56.00 AFV.select :carrierSolution
@06:30:56.11 delay 2 seconds
@06:30:58.14 AS.reconfigure ASconfig
@06:30:58.19 AS.seek 0.5ml
@06:31:27.20 delay 1 second
@06:31:28.22 AFV.select :holdingCoil
@06:31:28.32 ATV.dial :extSrc
@06:31:28.74 AS.reconfigure ASslow1
@06:31:28.82 AS.seek 0ml
@06:33:09.67 AFV.select :carrierSolution
@06:33:09.78 delay 2 seconds
@06:33:11.81 AS.reconfigure ASconfig
@06:33:11.86 AS.seek 0.5ml
@06:33:40.90 delay 1 second
@06:33:41.92 AFV.select :holdingCoil
@06:33:42.02 ATV.dial :extSrc
@06:33:42.04 AS.reconfigure ASslow1
@06:33:42.11 AS.seek 0ml
@06:35:22.95 ATV.dial :air2
@06:35:24.34 AS.reconfigure ASslow0
@06:35:24.40 AS.seek 0.5ml
@06:36:15.04 ATV.dial :extSrc
@06:36:16.60 AS.seek 0.02ml
@06:37:05.20 delay 1 second
@06:37:06.22 ATV.dial :waste
@06:37:07.57 AS.reconfigure ASconfig
@06:37:07.63 AS.seek 0ml
@06:37:09.07 deconEluateRecoveryLine extSrc
@06:37:09.08 ATV.dial :waste
@06:37:09.11 AS.reconfigure ASnorm
@06:37:09.17 AS.seek 0ml
@06:37:09.31 ATV.dial :air3
@06:37:10.20 AS.reconfigure ASslow1
@06:37:10.26 AS.seek 0.01ml
@06:37:12.68 delay 2 seconds
@06:37:14.70 ATV.dial :bleach
@06:37:15.20 AS.seek 0.31ml
@06:38:15.82 delay 3 seconds
@06:38:18.84 ATV.dial :air3
@06:38:19.45 delay 3 seconds
@06:38:22.52 AS.seek 0.335ml
@06:38:27.74 delay 3 seconds
@06:38:30.76 ATV.dial :bleach
@06:38:31.41 AS.seek 0.32ml
@06:38:34.70 delay 3 seconds
@06:38:37.72 ATV.dial :extSrc
@06:38:38.37 AS.seek 0ml
@06:39:43.01 AFV.select :carrierSolution
@06:39:43.12 delay 2 seconds
@06:39:45.15 AS.reconfigure ASconfig
@06:39:45.20 AS.seek 0.5ml
@06:40:14.25 delay 1 second
@06:40:15.27 AFV.select :holdingCoil
@06:40:15.37 ATV.dial :extSrc
@06:40:15.39 AS.reconfigure ASslow1
@06:40:15.45 AS.seek 0ml
@06:41:56.45 AFV.select :carrierSolution
@06:41:56.56 delay 2 seconds
@06:41:58.59 AS.reconfigure ASconfig
@06:41:58.64 AS.seek 0.5ml
@06:42:27.67 delay 1 second
@06:42:28.69 AFV.select :holdingCoil
@06:42:28.79 ATV.dial :extSrc
@06:42:28.81 AS.reconfigure ASslow1
@06:42:28.87 AS.seek 0ml
@06:44:09.72 AFV.select :carrierSolution
@06:44:09.83 delay 2 seconds
@06:44:11.86 AS.reconfigure ASconfig
@06:44:11.91 AS.seek 0.5ml
@06:44:40.93 delay 1 second
@06:44:41.95 AFV.select :holdingCoil
@06:44:42.05 ATV.dial :extSrc
@06:44:42.07 AS.reconfigure ASslow1
@06:44:42.13 AS.seek 0ml
@06:46:22.98 AFV.select :carrierSolution
@06:46:23.09 delay 2 seconds
@06:46:25.12 AS.reconfigure ASconfig
@06:46:25.17 AS.seek 0.5ml
@06:46:54.22 delay 1 second
@06:46:55.24 AFV.select :holdingCoil
@06:46:55.34 ATV.dial :extSrc
@06:46:55.36 AS.reconfigure ASslow1
@06:46:55.42 AS.seek 0ml
@06:48:36.25 ATV.dial :air2
@06:48:37.66 AS.reconfigure ASslow0
@06:48:37.72 AS.seek 0.5ml
@06:49:28.37 ATV.dial :extSrc
@06:49:29.93 AS.seek 0.02ml
@06:50:18.53 delay 1 second
@06:50:19.55 ATV.dial :waste
@06:50:20.92 AS.reconfigure ASconfig
@06:50:20.98 AS.seek 0ml
@06:50:22.46 <bkha> Session Ended
@13:03:42.51CEST04-May-21 <MAIN> ESP2 v3.30 I2C Gateway v3.06 for core bus
@13:03:44.64 
Configuration for mer 7/20/20 brent@mbari.org
/home/esp/esp2/type/shallow/mer/configure.rb
@13:03:44.73 
Configuration for MFB3 by preston@mbari.org
/home/esp/esp2/type/mfb/MFB3/configure.rb
@13:03:50.02 Polling Can every 10:00
@13:03:51.73 CS.configure :default
@13:03:51.77 CS.hold
@13:03:51.81 CS.configure :default
@13:03:51.89 PS.configure :default
@13:03:51.97 SS.configure :default
@13:03:52.01 SS.hold
@13:03:52.07 SS.configure :default
@13:03:52.15 CC.configure :default
@13:03:52.18 CC.hold
@13:03:52.26 CC.configure :default
@13:03:52.34 PC.configure :default
@13:03:52.37 PC.hold
@13:03:52.42 PC.configure :default
@13:03:52.52 Forearm.configure :loose
@13:03:52.55 Forearm.hold
@13:03:52.61 Forearm.configure :loose
@13:03:52.68 Elbow.configure :loose
@13:03:52.72 Elbow.hold
@13:03:52.76 Elbow.configure :loose
@13:03:52.86 SC.configure :loose
@13:03:52.90 SC.hold
@13:03:52.95 SC.configure :loose
@13:03:53.02 SE.configure :default
@13:03:53.06 SE.hold
@13:03:53.11 SE.configure :default
@13:03:53.14 CH.coast
@13:03:53.19 CH.configure :default
@13:03:53.21 PH.coast
@13:03:53.26 PH.configure :default
@13:03:53.36 SPE.coast
@13:03:53.41 SPE.configure SPEconfig
@13:03:53.48 AS.configure ASconfig
@13:03:53.52 AS.hold
@13:03:53.58 AS.configure ASconfig
@13:03:53.62 PCR.off
@13:03:53.65 PCR.pidtemp= 50,0.1,0,300,25,25
@13:03:53.68 PCR.caltemp= 2.5051,0.0372,0
@13:03:53.71 All dwarf controllers running firmware version 3.75
@13:03:53.94 Cleanly exited at 06:50:22CEST29-Apr-21
@13:03:53.97 Loading /home/esp/esp2/mission/stdMER2017.rb mission
@13:03:54.94 <mwj> Session Begun
@13:03:55.13 <MAIN> AFV.select :carrierSolution
@13:03:55.26 delay 2 seconds
@13:03:57.30 AS.seek 0.5ml
@13:04:26.33 delay 1 second
@13:04:27.34 AFV.select :holdingCoil
@13:04:27.44 ATV.dial :extSrc
@13:04:29.05 AS.reconfigure ASslow1
@13:04:29.11 AS.seek 0.53ml
@13:04:35.45 delay 5 seconds
@13:04:40.52 AS.reconfigure ASslow2
@13:04:40.58 AS.seek 0.54ml
@13:04:51.02 delay 20 seconds
@13:05:11.09 AS.reconfigure ASslow1
@13:05:11.15 AS.seek 0.57ml
@13:05:17.44 delay 5 seconds
@13:05:22.56 AS.reconfigure ASslow2
@13:05:22.67 AS.seek 0.58ml
@13:05:33.31 delay 20 seconds
@13:05:53.38 AS.reconfigure ASslow1
@13:05:53.44 AS.seek 0.61ml
@13:05:59.74 delay 5 seconds
@13:06:04.82 AS.reconfigure ASslow2
@13:06:04.87 AS.seek 0.62ml
@13:06:15.16 delay 20 seconds
@13:06:35.23 AS.reconfigure ASslow1
@13:06:35.28 AS.seek 0.65ml
@13:06:41.66 delay 5 seconds
@13:06:46.73 AS.reconfigure ASslow2
@13:06:46.79 AS.seek 0.66ml
@13:06:57.36 delay 20 seconds
@13:07:17.39 AS.reconfigure ASslow1
@13:07:17.44 AS.seek 0.705ml
@13:07:26.77 delay 10 seconds
@13:07:36.79 ATV.dial :air1
@13:07:38.99 AS.seek 0.725ml
@13:07:43.28 delay 3 seconds
@13:07:46.30 ATV.dial :waste
@13:07:46.89 AS.reconfigure ASslow0
@13:07:46.95 AS.seek 0ml
@13:09:00.27 <MFBPCR> Setting up PCR for AALPP3 -- writing /var/log/esp/AALPP321may0413h.pcr
@13:09:00.29 Building PCR Slug from extSrc with MM2 mix, PP3 primer
@13:09:00.32 Priming with carrier
@13:09:00.33 ATV.dial ARV
@13:09:01.94 ARV.dial :carrier
@13:09:02.31 AS.reconfigure ASnorm
@13:09:02.37 AS.seek 0.5ml
@13:09:27.89 AFV.select :carrierSolution
@13:09:28.00 delay 2 seconds
@13:09:30.03 AS.reconfigure ASconfig
@13:09:30.09 AS.seek 1ml
@13:09:59.13 delay 1 second
@13:10:00.15 AFV.select :holdingCoil
@13:10:00.27 delay 1 second
@13:10:01.29 ATV.dial :waste
@13:10:03.08 AS.reconfigure ASnorm
@13:10:03.14 AS.seek 0ml
@13:10:53.83 AFV.select :carrierSolution
@13:10:53.95 delay 2 seconds
@13:10:55.98 AS.reconfigure ASconfig
@13:10:56.03 AS.seek 0.5ml
@13:11:25.06 delay 1 second
@13:11:26.08 AFV.select :holdingCoil
@13:11:26.18 Building Slug
@13:11:26.20 ATV.dial :ARV
@13:11:27.97 delay 2 seconds
@13:11:29.99 ARV.dial :air1
@13:11:30.42 delay 2 seconds
@13:11:32.49 AS.reconfigure ASslow2
@13:11:32.55 AS.seek 0.506ml
@13:11:39.03 delay 2 seconds
@13:11:41.05 ARV.dial :MM2
@13:11:42.72 delay 2 seconds
@13:11:44.74 Adding MM2
@13:11:44.82 AS.seek 0.524ml
@13:12:03.25 delay 25 seconds
@13:12:28.27 ARV.dial :PP3
@13:12:28.70 Adding PP3
@13:12:28.71 delay 2 seconds
@13:12:30.79 AS.seek 0.53ml
@13:12:37.19 delay 10 seconds
@13:12:47.22 ARV.dial :air2
@13:12:47.66 adding ARV air
@13:12:47.67 delay 2 seconds
@13:12:49.75 AS.seek 0.536ml
@13:12:56.52 delay 5 seconds
@13:13:01.54 ARV.dial :carrier
@13:13:02.95 delay 7 seconds
@13:13:10.02 AS.seek 0.594ml
@13:14:08.80 delay 5 seconds
@13:14:13.82 ATV.dial :extSrc
@13:14:14.42 delay 2 seconds
@13:14:16.49 AS.seek 0.6ml
@13:14:23.02 delay 4 seconds
@13:14:27.04 ATV.dial ARV
@13:14:27.63 delay 2 seconds
@13:14:29.65 ARV.dial :carrier
@13:14:29.66 delay 2 seconds
@13:14:31.74 AS.seek 0.65ml
@13:15:22.20 delay 4 seconds
@13:15:26.22 ATV.dial PCR
@13:15:27.05 ASV.dial :waste1
@13:15:27.14 ARV.dial :air1
@13:15:27.55 Positioning Slug by 0.324ml
@13:15:27.64 AS.seek 0.326ml
@13:20:53.95 delay 5 seconds
@13:20:58.97 ATV.dial ARV
@13:20:59.78 ASV.dial :PCRlock
@13:21:00.19 delay 5 seconds
@13:21:05.21 Running AALPP3 Profile58CIQ2 thermocycling
@13:21:05.23 <PCR> PCR.program Profile58CIQ2
@13:21:05.34 Profile58CIQ2 should complete in < 1:36:15
@13:21:05.35 PCR.run 2:05:18.75
@14:42:24.94 PCR.off
@14:42:25.52 <MFBPCR> Running AALPP3 MeltProfile58 thermocycling
@14:42:25.53 <PCR> PCR.program MeltProfile58
@14:42:25.63 MeltProfile58 should complete in < 12:33
@14:42:25.64 PCR.run 20:41.25
@14:50:10.37 PCR.off
@14:50:11.36 <MFBPCR> PCR for AALPP3 completed -- closed /var/log/esp/AALPP321may0413h.pcr
@14:50:11.43 Cleaning PCR
@14:50:11.44 AFV.select :carrierSolution
@14:50:11.56 delay 2 seconds
@14:50:13.59 AS.reconfigure ASconfig
@14:50:13.64 AS.seek 0.5ml
@14:50:23.92 delay 1 second
@14:50:24.94 AFV.select :holdingCoil
@14:50:25.04 ASV.dial :waste1
@14:50:25.44 ATV.dial :air3
@14:50:26.80 AS.reconfigure ASslow1
@14:50:26.86 AS.seek 0.51ml
@14:50:29.15 delay 2 seconds
@14:50:31.17 ATV.dial :bleach
@14:50:31.83 AS.seek 0.81ml
@14:51:32.42 delay 3 seconds
@14:51:35.44 ATV.dial :air3
@14:51:36.03 delay 3 seconds
@14:51:39.10 AS.seek 0.835ml
@14:51:44.40 delay 3 seconds
@14:51:47.42 ATV.dial :bleach
@14:51:48.07 AS.seek 0.82ml
@14:51:51.37 delay 3 seconds
@14:51:54.39 ARV.dial :waste
@14:51:55.05 delay 3 seconds
@14:51:58.07 ATV.dial :ARV
@14:51:59.00 AS.reconfigure ASconfig
@14:51:59.06 AS.seek 0.47ml
@14:52:19.48 AFV.select :carrierSolution
@14:52:19.59 delay 2 seconds
@14:52:21.62 AS.seek 1ml
@14:52:52.39 delay 1 second
@14:52:53.77 AFV.select :holdingCoil
@14:52:53.87 delay 1 second
@14:52:54.90 AS.reconfigure ASfast
@14:52:54.95 AS.seek 0ml
@14:53:22.28 AFV.select :carrierSolution
@14:53:22.39 delay 2 seconds
@14:53:24.42 AS.reconfigure ASconfig
@14:53:24.47 AS.seek 1ml
@14:54:22.20 delay 1 second
@14:54:23.22 AFV.select :holdingCoil
@14:54:23.32 delay 1 second
@14:54:24.35 AS.reconfigure ASfast
@14:54:24.40 AS.seek 0ml
@14:54:51.75 AFV.select :carrierSolution
@14:54:51.86 delay 2 seconds
@14:54:53.88 AS.reconfigure ASconfig
@14:54:53.94 AS.seek 1ml
@14:55:51.67 delay 1 second
@14:55:52.69 AFV.select :holdingCoil
@14:55:52.79 delay 1 second
@14:55:53.82 AS.reconfigure ASfast
@14:55:53.87 AS.seek 0ml
@14:56:21.23 ARV.dial between carrier and air1
@14:56:21.77 ATV.dial :air3
@14:56:23.11 AS.reconfigure ASslow1
@14:56:23.17 AS.seek 0.01ml
@14:56:25.45 delay 2 seconds
@14:56:27.47 ATV.dial :bleach
@14:56:28.11 AS.seek 0.81ml
@14:59:09.23 delay 3 seconds
@14:59:12.25 ATV.dial :air3
@14:59:12.84 delay 3 seconds
@14:59:15.91 AS.seek 0.835ml
@14:59:21.23 delay 3 seconds
@14:59:24.25 ATV.dial :bleach
@14:59:24.89 AS.seek 0.82ml
@14:59:28.31 delay 3 seconds
@14:59:31.33 ATV.dial PCR
@14:59:31.77 AS.reconfigure ASconfig
@14:59:31.83 AS.seek 0ml
@15:00:19.23 PCR.program :bleachProfile
@15:00:19.30 PCR program should complete in < 2:45
@15:00:19.31 PCR.run 10:00
@15:03:15.09 PCR.off
@15:03:15.14 AFV.select :carrierSolution
@15:03:15.24 delay 2 seconds
@15:03:17.27 AS.seek 1ml
@15:04:15.02 delay 1 second
@15:04:16.04 AFV.select :holdingCoil
@15:04:16.14 delay 1 second
@15:04:17.17 AS.reconfigure ASfast
@15:04:17.22 AS.seek 0ml
@15:04:44.55 delay 3 seconds
@15:04:47.57 AFV.select :carrierSolution
@15:04:47.67 delay 2 seconds
@15:04:49.69 AS.reconfigure ASconfig
@15:04:49.76 AS.seek 1ml
@15:05:47.55 delay 1 second
@15:05:48.57 AFV.select :holdingCoil
@15:05:48.67 delay 1 second
@15:05:49.70 AS.reconfigure ASfast
@15:05:49.75 AS.seek 0ml
@15:06:17.09 delay 3 seconds
@15:06:20.11 AFV.select :carrierSolution
@15:06:20.21 delay 2 seconds
@15:06:22.23 AS.reconfigure ASconfig
@15:06:22.30 AS.seek 1ml
@15:07:20.09 delay 1 second
@15:07:21.11 AFV.select :holdingCoil
@15:07:21.21 delay 1 second
@15:07:22.24 AS.reconfigure ASfast
@15:07:22.29 AS.seek 0ml
@15:07:49.64 delay 3 seconds
@15:07:52.66 AFV.select :carrierSolution
@15:07:52.76 delay 2 seconds
@15:07:54.78 AS.reconfigure ASconfig
@15:07:54.85 AS.seek 1ml
@15:08:52.64 delay 1 second
@15:08:53.66 AFV.select :holdingCoil
@15:08:53.76 delay 1 second
@15:08:54.79 AS.reconfigure ASfast
@15:08:54.84 AS.seek 0ml
@15:09:22.17 delay 3 seconds
@15:09:25.19 AFV.select :carrierSolution
@15:09:25.29 delay 2 seconds
@15:09:27.31 AS.reconfigure ASconfig
@15:09:27.38 AS.seek 1ml
@15:10:25.12 delay 1 second
@15:10:26.14 AFV.select :holdingCoil
@15:10:26.24 delay 1 second
@15:10:27.27 AS.reconfigure ASnorm
@15:10:27.32 AS.seek 0ml
@15:11:18.05 <MAIN> AFV.select :carrierSolution
@15:11:18.15 delay 2 seconds
@15:11:20.18 AS.reconfigure ASconfig
@15:11:20.23 AS.seek 0.5ml
@15:11:49.30 delay 1 second
@15:11:50.32 AFV.select :holdingCoil
@15:11:50.42 ATV.dial :air1
@15:11:52.00 AS.reconfigure ASslow2
@15:11:52.06 AS.seek 0.506ml
@15:11:59.08 ATV.dial :extSrc
@15:12:00.88 AS.seek 0.512ml
@15:12:07.19 delay 5 seconds
@15:12:12.21 ATV.dial :air1
@15:12:14.03 AS.reconfigure ASslow1
@15:12:14.09 AS.seek 0.542ml
@15:12:20.37 ATV.dial :waste
@15:12:20.95 AS.reconfigure ASconfig
@15:12:21.01 AS.seek 0ml
@15:12:52.61 <MFBPCR> Setting up PCR for AALPP3 -- writing /var/log/esp/AALPP321may0415h.pcr
@15:12:52.63 Building PCR Slug from extSrc with MM2 mix, PP3 primer
@15:12:52.66 Priming with carrier
@15:12:52.67 ATV.dial ARV
@15:12:54.27 ARV.dial :carrier
@15:12:54.62 AS.reconfigure ASnorm
@15:12:54.68 AS.seek 0.5ml
@15:13:20.28 AFV.select :carrierSolution
@15:13:20.40 delay 2 seconds
@15:13:22.43 AS.reconfigure ASconfig
@15:13:22.49 AS.seek 1ml
@15:13:51.53 delay 1 second
@15:13:52.55 AFV.select :holdingCoil
@15:13:52.65 delay 1 second
@15:13:53.67 ATV.dial :waste
@15:13:55.47 AS.reconfigure ASnorm
@15:13:55.53 AS.seek 0ml
@15:14:46.25 AFV.select :carrierSolution
@15:14:46.36 delay 2 seconds
@15:14:48.39 AS.reconfigure ASconfig
@15:14:48.44 AS.seek 0.5ml
@15:15:17.51 delay 1 second
@15:15:18.53 AFV.select :holdingCoil
@15:15:18.63 Building Slug
@15:15:18.64 ATV.dial :ARV
@15:15:20.41 delay 2 seconds
@15:15:22.43 ARV.dial :air1
@15:15:22.84 delay 2 seconds
@15:15:24.91 AS.reconfigure ASslow2
@15:15:24.97 AS.seek 0.506ml
@15:15:31.44 delay 2 seconds
@15:15:33.46 ARV.dial :MM2
@15:15:35.09 delay 2 seconds
@15:15:37.11 Adding MM2
@15:15:37.18 AS.seek 0.524ml
@15:15:55.86 delay 25 seconds
@15:16:20.88 ARV.dial :PP3
@15:16:21.30 Adding PP3
@15:16:21.31 delay 2 seconds
@15:16:23.39 AS.seek 0.53ml
@15:16:30.01 delay 10 seconds
@15:16:40.04 ARV.dial :air2
@15:16:40.47 adding ARV air
@15:16:40.48 delay 2 seconds
@15:16:42.56 AS.seek 0.536ml
@15:16:48.89 delay 5 seconds
@15:16:53.91 ARV.dial :carrier
@15:16:55.31 delay 7 seconds
@15:17:02.38 AS.seek 0.594ml
@15:18:01.33 delay 5 seconds
@15:18:06.35 ATV.dial :extSrc
@15:18:06.95 delay 2 seconds
@15:18:09.02 AS.seek 0.6ml
@15:18:15.30 delay 4 seconds
@15:18:19.32 ATV.dial ARV
@15:18:19.91 delay 2 seconds
@15:18:21.93 ARV.dial :carrier
@15:18:21.94 delay 2 seconds
@15:18:24.02 AS.seek 0.65ml
@15:19:14.56 delay 4 seconds
@15:19:18.58 ATV.dial PCR
@15:19:19.41 ASV.dial :waste1
@15:19:19.42 ARV.dial :air1
@15:19:19.88 Positioning Slug by 0.324ml
@15:19:19.96 AS.seek 0.326ml
@15:24:46.37 delay 5 seconds
@15:24:51.39 ATV.dial ARV
@15:24:52.20 ASV.dial :PCRlock
@15:24:52.59 delay 5 seconds
@15:24:57.61 Running AALPP3 Profile58CIQ2 thermocycling
@15:24:57.62 <PCR> PCR.program Profile58CIQ2
@15:24:57.74 Profile58CIQ2 should complete in < 1:36:15
@15:24:57.75 PCR.run 2:05:18.75
@16:46:15.17 PCR.off
@16:46:15.74 <MFBPCR> Running AALPP3 MeltProfile58 thermocycling
@16:46:15.75 <PCR> PCR.program MeltProfile58
@16:46:15.85 MeltProfile58 should complete in < 12:33
@16:46:15.86 PCR.run 20:41.25
@16:54:06.64 PCR.off
@16:54:07.63 <MFBPCR> PCR for AALPP3 completed -- closed /var/log/esp/AALPP321may0415h.pcr
@16:54:07.70 Cleaning PCR
@16:54:07.71 AFV.select :carrierSolution
@16:54:07.83 delay 2 seconds
@16:54:09.86 AS.reconfigure ASconfig
@16:54:09.91 AS.seek 0.5ml
@16:54:20.20 delay 1 second
@16:54:21.22 AFV.select :holdingCoil
@16:54:21.32 ASV.dial :waste1
@16:54:21.72 ATV.dial :air3
@16:54:23.08 AS.reconfigure ASslow1
@16:54:23.14 AS.seek 0.51ml
@16:54:25.48 delay 2 seconds
@16:54:27.50 ATV.dial :bleach
@16:54:28.14 AS.seek 0.81ml
@16:55:28.75 delay 3 seconds
@16:55:31.77 ATV.dial :air3
@16:55:32.36 delay 3 seconds
@16:55:35.43 AS.seek 0.835ml
@16:55:40.70 delay 3 seconds
@16:55:43.72 ATV.dial :bleach
@16:55:44.36 AS.seek 0.82ml
@16:55:47.78 delay 3 seconds
@16:55:50.80 ARV.dial :waste
@16:55:51.45 delay 3 seconds
@16:55:54.47 ATV.dial :ARV
@16:55:55.77 AS.reconfigure ASconfig
@16:55:55.83 AS.seek 0.47ml
@16:56:16.26 AFV.select :carrierSolution
@16:56:16.36 delay 2 seconds
@16:56:18.39 AS.seek 1ml
@16:56:49.14 delay 1 second
@16:56:50.16 AFV.select :holdingCoil
@16:56:50.26 delay 1 second
@16:56:51.29 AS.reconfigure ASfast
@16:56:51.34 AS.seek 0ml
@16:57:18.70 AFV.select :carrierSolution
@16:57:18.81 delay 2 seconds
@16:57:20.84 AS.reconfigure ASconfig
@16:57:20.89 AS.seek 1ml
@16:58:18.62 delay 1 second
@16:58:19.64 AFV.select :holdingCoil
@16:58:19.74 delay 1 second
@16:58:20.77 AS.reconfigure ASfast
@16:58:20.82 AS.seek 0ml
@16:58:48.17 AFV.select :carrierSolution
@16:58:48.28 delay 2 seconds
@16:58:50.31 AS.reconfigure ASconfig
@16:58:50.36 AS.seek 1ml
@16:59:48.19 delay 1 second
@16:59:49.21 AFV.select :holdingCoil
@16:59:49.31 delay 1 second
@16:59:50.34 AS.reconfigure ASfast
@16:59:50.39 AS.seek 0ml
@17:00:17.75 ARV.dial between carrier and air1
@17:00:18.28 ATV.dial :air3
@17:00:19.64 AS.reconfigure ASslow1
@17:00:19.70 AS.seek 0.01ml
@17:00:22.20 delay 2 seconds
@17:00:24.22 ATV.dial :bleach
@17:00:24.88 AS.seek 0.81ml
@17:03:06.05 delay 3 seconds
@17:03:09.07 ATV.dial :air3
@17:03:09.65 delay 3 seconds
@17:03:12.72 AS.seek 0.835ml
@17:03:18.04 delay 3 seconds
@17:03:21.06 ATV.dial :bleach
@17:03:21.70 AS.seek 0.82ml
@17:03:25.01 delay 3 seconds
@17:03:28.03 ATV.dial PCR
@17:03:28.47 AS.reconfigure ASconfig
@17:03:28.53 AS.seek 0ml
@17:04:15.94 PCR.program :bleachProfile
@17:04:16.00 PCR program should complete in < 2:45
@17:04:16.01 PCR.run 10:00
@17:07:11.81 PCR.off
@17:07:11.85 AFV.select :carrierSolution
@17:07:11.96 delay 2 seconds
@17:07:13.99 AS.seek 1ml
@17:08:11.78 delay 1 second
@17:08:12.80 AFV.select :holdingCoil
@17:08:12.90 delay 1 second
@17:08:13.93 AS.reconfigure ASfast
@17:08:13.98 AS.seek 0ml
@17:08:41.31 delay 3 seconds
@17:08:44.33 AFV.select :carrierSolution
@17:08:44.43 delay 2 seconds
@17:08:46.45 AS.reconfigure ASconfig
@17:08:46.52 AS.seek 1ml
@17:09:44.26 delay 1 second
@17:09:45.28 AFV.select :holdingCoil
@17:09:45.38 delay 1 second
@17:09:46.41 AS.reconfigure ASfast
@17:09:46.46 AS.seek 0ml
@17:10:13.81 delay 3 seconds
@17:10:16.83 AFV.select :carrierSolution
@17:10:16.93 delay 2 seconds
@17:10:18.95 AS.reconfigure ASconfig
@17:10:19.02 AS.seek 1ml
@17:11:16.81 delay 1 second
@17:11:17.83 AFV.select :holdingCoil
@17:11:17.93 delay 1 second
@17:11:18.96 AS.reconfigure ASfast
@17:11:19.01 AS.seek 0ml
@17:11:46.33 delay 3 seconds
@17:11:49.35 AFV.select :carrierSolution
@17:11:49.45 delay 2 seconds
@17:11:51.47 AS.reconfigure ASconfig
@17:11:51.53 AS.seek 1ml
@17:12:49.30 delay 1 second
@17:12:50.32 AFV.select :holdingCoil
@17:12:50.42 delay 1 second
@17:12:51.45 AS.reconfigure ASfast
@17:12:51.50 AS.seek 0ml
@17:13:18.84 delay 3 seconds
@17:13:21.86 AFV.select :carrierSolution
@17:13:21.96 delay 2 seconds
@17:13:23.98 AS.reconfigure ASconfig
@17:13:24.05 AS.seek 1ml
@17:14:21.84 delay 1 second
@17:14:22.86 AFV.select :holdingCoil
@17:14:22.96 delay 1 second
@17:14:23.98 AS.reconfigure ASnorm
@17:14:24.04 AS.seek 0ml
@17:15:15.11 <MAIN> AFV.select :carrierSolution
@17:15:15.22 delay 2 seconds
@17:15:17.25 AS.reconfigure ASconfig
@17:15:17.30 AS.seek 0.5ml
@17:15:46.33 delay 1 second
@17:15:47.35 AFV.select :holdingCoil
@17:15:47.45 ATV.dial :air1
@17:15:49.03 AS.reconfigure ASslow2
@17:15:49.09 AS.seek 0.51ml
@17:15:59.69 delay 5 seconds
@17:16:04.71 ATV.dial :extSrc
@17:16:06.52 AS.seek 0.53ml
@17:16:27.04 delay 10 seconds
@17:16:37.06 ATV.dial :air1
@17:16:38.88 AS.reconfigure ASslow1
@17:16:38.94 AS.seek 0.56ml
@17:16:45.26 ATV.dial :waste
@17:16:45.83 AS.reconfigure ASconfig
@17:16:45.89 AS.seek 0ml
@17:17:18.52 <MFBPCR> Setting up PCR for AALPP3 -- writing /var/log/esp/AALPP321may0417h.pcr
@17:17:18.55 Building PCR Slug from extSrc with MM2 mix, PP3 primer
@17:17:18.57 Priming with carrier
@17:17:18.59 ATV.dial ARV
@17:17:20.17 ARV.dial :carrier
@17:17:20.53 AS.reconfigure ASnorm
@17:17:20.59 AS.seek 0.5ml
@17:17:46.17 AFV.select :carrierSolution
@17:17:46.28 delay 2 seconds
@17:17:48.31 AS.reconfigure ASconfig
@17:17:48.37 AS.seek 1ml
@17:18:17.40 delay 1 second
@17:18:18.42 AFV.select :holdingCoil
@17:18:18.52 delay 1 second
@17:18:19.54 ATV.dial :waste
@17:18:21.33 AS.reconfigure ASnorm
@17:18:21.39 AS.seek 0ml
@17:19:12.09 AFV.select :carrierSolution
@17:19:12.20 delay 2 seconds
@17:19:14.23 AS.reconfigure ASconfig
@17:19:14.28 AS.seek 0.5ml
@17:19:43.36 delay 1 second
@17:19:44.38 AFV.select :holdingCoil
@17:19:44.48 Building Slug
@17:19:44.50 ATV.dial :ARV
@17:19:46.36 delay 2 seconds
@17:19:48.38 ARV.dial :air1
@17:19:48.81 delay 2 seconds
@17:19:50.88 AS.reconfigure ASslow2
@17:19:50.94 AS.seek 0.506ml
@17:19:57.29 delay 2 seconds
@17:19:59.31 ARV.dial :MM2
@17:20:00.94 delay 2 seconds
@17:20:02.96 Adding MM2
@17:20:03.04 AS.seek 0.524ml
@17:20:21.59 delay 25 seconds
@17:20:46.61 ARV.dial :PP3
@17:20:47.03 Adding PP3
@17:20:47.04 delay 2 seconds
@17:20:49.12 AS.seek 0.53ml
@17:20:55.61 delay 10 seconds
@17:21:05.65 ARV.dial :air2
@17:21:06.08 adding ARV air
@17:21:06.09 delay 2 seconds
@17:21:08.17 AS.seek 0.536ml
@17:21:14.59 delay 5 seconds
@17:21:19.61 ARV.dial :carrier
@17:21:21.00 delay 7 seconds
@17:21:28.07 AS.seek 0.594ml
@17:22:26.65 delay 5 seconds
@17:22:31.67 ATV.dial :extSrc
@17:22:32.26 delay 2 seconds
@17:22:34.33 AS.seek 0.6ml
@17:22:40.64 delay 4 seconds
@17:22:44.66 ATV.dial ARV
@17:22:45.23 delay 2 seconds
@17:22:47.25 ARV.dial :carrier
@17:22:47.26 delay 2 seconds
@17:22:49.34 AS.seek 0.65ml
@17:23:40.08 delay 4 seconds
@17:23:44.10 ATV.dial PCR
@17:23:44.92 ASV.dial :waste1
@17:23:44.93 ARV.dial :air1
@17:23:45.37 Positioning Slug by 0.324ml
@17:23:45.46 AS.seek 0.326ml
@17:29:11.84 delay 5 seconds
@17:29:16.86 ATV.dial ARV
@17:29:17.69 ASV.dial :PCRlock
@17:29:18.09 delay 5 seconds
@17:29:23.11 Running AALPP3 Profile58CIQ2 thermocycling
@17:29:23.13 <PCR> PCR.program Profile58CIQ2
@17:29:23.24 Profile58CIQ2 should complete in < 1:36:15
@17:29:23.25 PCR.run 2:05:18.75
@18:50:34.47 PCR.off
@18:50:35.04 <MFBPCR> Running AALPP3 MeltProfile58 thermocycling
@18:50:35.06 <PCR> PCR.program MeltProfile58
@18:50:35.16 MeltProfile58 should complete in < 12:33
@18:50:35.17 PCR.run 20:41.25
@18:58:19.53 PCR.off
@18:58:20.88 <MFBPCR> PCR for AALPP3 completed -- closed /var/log/esp/AALPP321may0417h.pcr
@18:58:20.95 Cleaning PCR
@18:58:20.96 AFV.select :carrierSolution
@18:58:21.08 delay 2 seconds
@18:58:23.11 AS.reconfigure ASconfig
@18:58:23.16 AS.seek 0.5ml
@18:58:33.45 delay 1 second
@18:58:34.47 AFV.select :holdingCoil
@18:58:34.57 ASV.dial :waste1
@18:58:34.97 ATV.dial :air3
@18:58:36.31 AS.reconfigure ASslow1
@18:58:36.37 AS.seek 0.51ml
@18:58:38.65 delay 2 seconds
@18:58:40.67 ATV.dial :bleach
@18:58:41.31 AS.seek 0.81ml
@18:59:41.92 delay 3 seconds
@18:59:44.94 ATV.dial :air3
@18:59:45.53 delay 3 seconds
@18:59:48.60 AS.seek 0.835ml
@18:59:53.95 delay 3 seconds
@18:59:56.97 ATV.dial :bleach
@18:59:57.61 AS.seek 0.82ml
@19:00:00.92 delay 3 seconds
@19:00:03.94 ARV.dial :waste
@19:00:04.61 delay 3 seconds
@19:00:07.63 ATV.dial :ARV
@19:00:08.56 AS.reconfigure ASconfig
@19:00:08.62 AS.seek 0.47ml
@19:00:29.01 AFV.select :carrierSolution
@19:00:29.12 delay 2 seconds
@19:00:31.15 AS.seek 1ml
@19:01:01.94 delay 1 second
@19:01:02.96 AFV.select :holdingCoil
@19:01:03.06 delay 1 second
@19:01:04.09 AS.reconfigure ASfast
@19:01:04.14 AS.seek 0ml
@19:01:31.48 AFV.select :carrierSolution
@19:01:31.59 delay 2 seconds
@19:01:33.62 AS.reconfigure ASconfig
@19:01:33.67 AS.seek 1ml
@19:02:31.40 delay 1 second
@19:02:32.42 AFV.select :holdingCoil
@19:02:32.52 delay 1 second
@19:02:33.55 AS.reconfigure ASfast
@19:02:33.60 AS.seek 0ml
@19:03:00.95 AFV.select :carrierSolution
@19:03:01.06 delay 2 seconds
@19:03:03.09 AS.reconfigure ASconfig
@19:03:03.14 AS.seek 1ml
@19:04:00.87 delay 1 second
@19:04:01.89 AFV.select :holdingCoil
@19:04:01.99 delay 1 second
@19:04:03.02 AS.reconfigure ASfast
@19:04:03.07 AS.seek 0ml
@19:04:30.42 ARV.dial between carrier and air1
@19:04:30.95 ATV.dial :air3
@19:04:32.31 AS.reconfigure ASslow1
@19:04:32.37 AS.seek 0.01ml
@19:04:34.65 delay 2 seconds
@19:04:36.67 ATV.dial :bleach
@19:04:37.33 AS.seek 0.81ml
@19:07:18.47 delay 3 seconds
@19:07:21.49 ATV.dial :air3
@19:07:22.09 delay 3 seconds
@19:07:25.16 AS.seek 0.835ml
@19:07:30.44 delay 3 seconds
@19:07:33.46 ATV.dial :bleach
@19:07:34.09 AS.seek 0.82ml
@19:07:37.39 delay 3 seconds
@19:07:40.41 ATV.dial PCR
@19:07:40.84 AS.reconfigure ASconfig
@19:07:40.90 AS.seek 0ml
@19:08:28.31 PCR.program :bleachProfile
@19:08:28.37 PCR program should complete in < 2:45
@19:08:28.38 PCR.run 10:00
@19:11:24.55 PCR.off
@19:11:24.60 AFV.select :carrierSolution
@19:11:24.71 delay 2 seconds
@19:11:26.74 AS.seek 1ml
@19:12:24.47 delay 1 second
@19:12:25.49 AFV.select :holdingCoil
@19:12:25.59 delay 1 second
@19:12:26.62 AS.reconfigure ASfast
@19:12:26.67 AS.seek 0ml
@19:12:54.00 delay 3 seconds
@19:12:57.02 AFV.select :carrierSolution
@19:12:57.12 delay 2 seconds
@19:12:59.15 AS.reconfigure ASconfig
@19:12:59.21 AS.seek 1ml
@19:13:56.98 delay 1 second
@19:13:58.00 AFV.select :holdingCoil
@19:13:58.10 delay 1 second
@19:13:59.13 AS.reconfigure ASfast
@19:13:59.18 AS.seek 0ml
@19:14:26.51 delay 3 seconds
@19:14:29.53 AFV.select :carrierSolution
@19:14:29.63 delay 2 seconds
@19:14:31.66 AS.reconfigure ASconfig
@19:14:31.72 AS.seek 1ml
@19:15:29.47 delay 1 second
@19:15:30.49 AFV.select :holdingCoil
@19:15:30.59 delay 1 second
@19:15:31.62 AS.reconfigure ASfast
@19:15:31.67 AS.seek 0ml
@19:15:59.01 delay 3 seconds
@19:16:02.03 AFV.select :carrierSolution
@19:16:02.13 delay 2 seconds
@19:16:04.16 AS.reconfigure ASconfig
@19:16:04.22 AS.seek 1ml
@19:17:01.97 delay 1 second
@19:17:02.99 AFV.select :holdingCoil
@19:17:03.09 delay 1 second
@19:17:04.12 AS.reconfigure ASfast
@19:17:04.17 AS.seek 0ml
@19:17:31.50 delay 3 seconds
@19:17:34.52 AFV.select :carrierSolution
@19:17:34.62 delay 2 seconds
@19:17:36.64 AS.reconfigure ASconfig
@19:17:36.71 AS.seek 1ml
@19:18:34.45 delay 1 second
@19:18:35.47 AFV.select :holdingCoil
@19:18:35.57 delay 1 second
@19:18:36.60 AS.reconfigure ASnorm
@19:18:36.65 AS.seek 0ml
@19:19:27.35 <MAIN> AFV.select :carrierSolution
@19:19:27.46 delay 2 seconds
@19:19:29.49 AS.reconfigure ASconfig
@19:19:29.54 AS.seek 0.5ml
@19:19:58.61 delay 1 second
@19:19:59.63 AFV.select :holdingCoil
@19:19:59.73 ATV.dial :air1
@19:20:01.33 AS.reconfigure ASslow2
@19:20:01.39 AS.seek 0.506ml
@19:20:07.93 ATV.dial :extSrc
@19:20:09.72 AS.seek 0.512ml
@19:20:16.04 delay 5 seconds
@19:20:21.06 ATV.dial :air1
@19:20:22.89 AS.reconfigure ASslow1
@19:20:22.95 AS.seek 0.542ml
@19:20:29.25 ATV.dial :waste
@19:20:29.83 AS.reconfigure ASconfig
@19:20:29.89 AS.seek 0ml
@19:21:01.48 <MFBPCR> Setting up PCR for AALPP3 -- writing /var/log/esp/AALPP321may0419h.pcr
@19:21:01.50 Building PCR Slug from extSrc with MM2 mix, PP3 primer
@19:21:01.53 Priming with carrier
@19:21:01.55 ATV.dial ARV
@19:21:03.12 ARV.dial :carrier
@19:21:03.46 AS.reconfigure ASnorm
@19:21:03.52 AS.seek 0.5ml
@19:21:29.14 AFV.select :carrierSolution
@19:21:29.26 delay 2 seconds
@19:21:31.29 AS.reconfigure ASconfig
@19:21:31.34 AS.seek 1ml
@19:22:00.36 delay 1 second
@19:22:01.38 AFV.select :holdingCoil
@19:22:01.48 delay 1 second
@19:22:02.50 ATV.dial :waste
@19:22:04.28 AS.reconfigure ASnorm
@19:22:04.34 AS.seek 0ml
@19:22:55.04 AFV.select :carrierSolution
@19:22:55.15 delay 2 seconds
@19:22:57.18 AS.reconfigure ASconfig
@19:22:57.23 AS.seek 0.5ml
@19:23:26.26 delay 1 second
@19:23:27.28 AFV.select :holdingCoil
@19:23:27.38 Building Slug
@19:23:27.39 ATV.dial :ARV
@19:23:29.17 delay 2 seconds
@19:23:31.19 ARV.dial :air1
@19:23:31.59 delay 2 seconds
@19:23:33.66 AS.reconfigure ASslow2
@19:23:33.72 AS.seek 0.506ml
@19:23:40.26 delay 2 seconds
@19:23:42.28 ARV.dial :MM2
@19:23:44.28 delay 2 seconds
@19:23:46.30 Adding MM2
@19:23:46.38 AS.seek 0.524ml
@19:24:05.12 delay 25 seconds
@19:24:30.14 ARV.dial :PP3
@19:24:30.56 Adding PP3
@19:24:30.57 delay 2 seconds
@19:24:32.65 AS.seek 0.53ml
@19:24:39.45 delay 10 seconds
@19:24:49.48 ARV.dial :air2
@19:24:49.90 adding ARV air
@19:24:49.91 delay 2 seconds
@19:24:51.99 AS.seek 0.536ml
@19:24:59.11 delay 5 seconds
@19:25:04.13 ARV.dial :carrier
@19:25:05.51 delay 7 seconds
@19:25:12.59 AS.seek 0.594ml
@19:26:11.26 delay 5 seconds
@19:26:16.28 ATV.dial :extSrc
@19:26:16.87 delay 2 seconds
@19:26:18.94 AS.seek 0.6ml
@19:26:25.17 delay 4 seconds
@19:26:29.19 ATV.dial ARV
@19:26:29.76 delay 2 seconds
@19:26:31.78 ARV.dial :carrier
@19:26:31.79 delay 2 seconds
@19:26:33.87 AS.seek 0.65ml
@19:27:24.40 delay 4 seconds
@19:27:28.42 ATV.dial PCR
@19:27:29.23 ASV.dial :waste1
@19:27:29.24 ARV.dial :air1
@19:27:29.67 Positioning Slug by 0.324ml
@19:27:29.76 AS.seek 0.326ml
@19:32:56.14 delay 5 seconds
@19:33:01.16 ATV.dial ARV
@19:33:01.98 ASV.dial :PCRlock
@19:33:02.39 delay 5 seconds
@19:33:07.41 Running AALPP3 Profile58CIQ2 thermocycling
@19:33:07.42 <PCR> PCR.program Profile58CIQ2
@19:33:07.54 Profile58CIQ2 should complete in < 1:36:15
@19:33:07.55 PCR.run 2:05:18.75
@20:54:14.84 PCR.off
@20:54:15.41 <MFBPCR> Running AALPP3 MeltProfile58 thermocycling
@20:54:15.42 <PCR> PCR.program MeltProfile58
@20:54:15.52 MeltProfile58 should complete in < 12:33
@20:54:15.53 PCR.run 20:41.25
@21:02:06.48 PCR.off
@21:02:07.47 <MFBPCR> PCR for AALPP3 completed -- closed /var/log/esp/AALPP321may0419h.pcr
@21:02:07.54 Cleaning PCR
@21:02:07.55 AFV.select :carrierSolution
@21:02:07.67 delay 2 seconds
@21:02:09.70 AS.reconfigure ASconfig
@21:02:09.75 AS.seek 0.5ml
@21:02:20.09 delay 1 second
@21:02:21.11 AFV.select :holdingCoil
@21:02:21.21 ASV.dial :waste1
@21:02:21.61 ATV.dial :air3
@21:02:22.97 AS.reconfigure ASslow1
@21:02:23.03 AS.seek 0.51ml
@21:02:25.34 delay 2 seconds
@21:02:27.36 ATV.dial :bleach
@21:02:28.02 AS.seek 0.81ml
@21:03:28.61 delay 3 seconds
@21:03:31.63 ATV.dial :air3
@21:03:32.23 delay 3 seconds
@21:03:35.30 AS.seek 0.835ml
@21:03:40.68 delay 3 seconds
@21:03:43.70 ATV.dial :bleach
@21:03:44.34 AS.seek 0.82ml
@21:03:47.56 delay 3 seconds
@21:03:50.58 ARV.dial :waste
@21:03:51.25 delay 3 seconds
@21:03:54.27 ATV.dial :ARV
@21:03:55.20 AS.reconfigure ASconfig
@21:03:55.26 AS.seek 0.47ml
@21:04:15.67 AFV.select :carrierSolution
@21:04:15.78 delay 2 seconds
@21:04:17.81 AS.seek 1ml
@21:04:48.54 delay 1 second
@21:04:49.56 AFV.select :holdingCoil
@21:04:49.66 delay 1 second
@21:04:50.69 AS.reconfigure ASfast
@21:04:50.74 AS.seek 0ml
@21:05:18.08 AFV.select :carrierSolution
@21:05:18.19 delay 2 seconds
@21:05:20.21 AS.reconfigure ASconfig
@21:05:20.27 AS.seek 1ml
@21:06:18.42 delay 1 second
@21:06:19.44 AFV.select :holdingCoil
@21:06:19.54 delay 1 second
@21:06:20.57 AS.reconfigure ASfast
@21:06:20.62 AS.seek 0ml
@21:06:47.97 AFV.select :carrierSolution
@21:06:48.08 delay 2 seconds
@21:06:50.11 AS.reconfigure ASconfig
@21:06:50.16 AS.seek 1ml
@21:07:47.95 delay 1 second
@21:07:48.97 AFV.select :holdingCoil
@21:07:49.07 delay 1 second
@21:07:50.10 AS.reconfigure ASfast
@21:07:50.15 AS.seek 0ml
@21:08:17.48 ARV.dial between carrier and air1
@21:08:18.01 ATV.dial :air3
@21:08:19.38 AS.reconfigure ASslow1
@21:08:19.44 AS.seek 0.01ml
@21:08:21.87 delay 2 seconds
@21:08:23.89 ATV.dial :bleach
@21:08:24.56 AS.seek 0.81ml
@21:11:05.72 delay 3 seconds
@21:11:08.74 ATV.dial :air3
@21:11:09.33 delay 3 seconds
@21:11:12.40 AS.seek 0.835ml
@21:11:17.68 delay 3 seconds
@21:11:20.70 ATV.dial :bleach
@21:11:21.34 AS.seek 0.82ml
@21:11:24.65 delay 3 seconds
@21:11:27.67 ATV.dial PCR
@21:11:28.09 AS.reconfigure ASconfig
@21:11:28.15 AS.seek 0ml
@21:12:15.56 PCR.program :bleachProfile
@21:12:15.62 PCR program should complete in < 2:45
@21:12:15.63 PCR.run 10:00
@21:15:11.55 PCR.off
@21:15:11.59 AFV.select :carrierSolution
@21:15:11.70 delay 2 seconds
@21:15:13.73 AS.seek 1ml
@21:16:11.53 delay 1 second
@21:16:12.55 AFV.select :holdingCoil
@21:16:12.68 delay 1 second
@21:16:13.71 AS.reconfigure ASfast
@21:16:13.76 AS.seek 0ml
@21:16:41.09 delay 3 seconds
@21:16:44.11 AFV.select :carrierSolution
@21:16:44.21 delay 2 seconds
@21:16:46.24 AS.reconfigure ASconfig
@21:16:46.30 AS.seek 1ml
@21:17:44.04 delay 1 second
@21:17:45.06 AFV.select :holdingCoil
@21:17:45.16 delay 1 second
@21:17:46.19 AS.reconfigure ASfast
@21:17:46.24 AS.seek 0ml
@21:18:13.58 delay 3 seconds
@21:18:16.60 AFV.select :carrierSolution
@21:18:16.70 delay 2 seconds
@21:18:18.72 AS.reconfigure ASconfig
@21:18:18.79 AS.seek 1ml
@21:19:16.53 delay 1 second
@21:19:17.55 AFV.select :holdingCoil
@21:19:17.65 delay 1 second
@21:19:18.68 AS.reconfigure ASfast
@21:19:18.73 AS.seek 0ml
@21:19:46.45 delay 3 seconds
@21:19:49.47 AFV.select :carrierSolution
@21:19:49.57 delay 2 seconds
@21:19:51.60 AS.reconfigure ASconfig
@21:19:51.65 AS.seek 1ml
@21:20:49.39 delay 1 second
@21:20:50.41 AFV.select :holdingCoil
@21:20:50.51 delay 1 second
@21:20:51.54 AS.reconfigure ASfast
@21:20:51.59 AS.seek 0ml
@21:21:18.93 delay 3 seconds
@21:21:21.95 AFV.select :carrierSolution
@21:21:22.05 delay 2 seconds
@21:21:24.08 AS.reconfigure ASconfig
@21:21:24.14 AS.seek 1ml
@21:22:21.89 delay 1 second
@21:22:22.91 AFV.select :holdingCoil
@21:22:23.01 delay 1 second
@21:22:24.04 AS.reconfigure ASnorm
@21:22:24.09 AS.seek 0ml
@21:23:14.81 <MAIN> AFV.select :carrierSolution
@21:23:14.92 delay 2 seconds
@21:23:16.95 AS.reconfigure ASconfig
@21:23:17.00 AS.seek 0.5ml
@21:23:46.08 delay 1 second
@21:23:47.10 AFV.select :holdingCoil
@21:23:47.20 ATV.dial :air1
@21:23:48.78 AS.reconfigure ASslow1
@21:23:48.84 AS.seek 0.52ml
@21:23:53.15 delay 5 seconds
@21:23:58.17 ATV.dial :extSrc
@21:23:59.97 AS.reconfigure ASslow2
@21:24:00.03 AS.seek 0.545ml
@21:24:25.45 delay 10 seconds
@21:24:35.47 ATV.dial :air1
@21:24:37.28 AS.reconfigure ASslow1
@21:24:37.34 AS.seek 0.575ml
@21:24:43.62 ATV.dial :waste
@21:24:44.20 AS.reconfigure ASconfig
@21:24:44.26 AS.seek 0ml
@21:25:17.76 <MFBPCR> Setting up PCR for AALPP3 -- writing /var/log/esp/AALPP321may0421h.pcr
@21:25:17.78 Building PCR Slug from extSrc with MM2 mix, PP3 primer
@21:25:17.80 Priming with carrier
@21:25:17.82 ATV.dial ARV
@21:25:19.40 ARV.dial :carrier
@21:25:19.74 AS.reconfigure ASnorm
@21:25:19.80 AS.seek 0.5ml
@21:25:45.39 AFV.select :carrierSolution
@21:25:45.51 delay 2 seconds
@21:25:47.54 AS.reconfigure ASconfig
@21:25:47.60 AS.seek 1ml
@21:26:16.68 delay 1 second
@21:26:17.70 AFV.select :holdingCoil
@21:26:17.80 delay 1 second
@21:26:18.82 ATV.dial :waste
@21:26:20.61 AS.reconfigure ASnorm
@21:26:20.67 AS.seek 0ml
@21:27:11.37 AFV.select :carrierSolution
@21:27:11.48 delay 2 seconds
@21:27:13.51 AS.reconfigure ASconfig
@21:27:13.56 AS.seek 0.5ml
@21:27:42.64 delay 1 second
@21:27:43.66 AFV.select :holdingCoil
@21:27:43.76 Building Slug
@21:27:43.78 ATV.dial :ARV
@21:27:45.54 delay 2 seconds
@21:27:47.56 ARV.dial :air1
@21:27:47.98 delay 2 seconds
@21:27:50.05 AS.reconfigure ASslow2
@21:27:50.11 AS.seek 0.506ml
@21:27:56.31 delay 2 seconds
@21:27:58.33 ARV.dial :MM2
@21:27:59.95 delay 2 seconds
@21:28:01.97 Adding MM2
@21:28:02.05 AS.seek 0.524ml
@21:28:20.81 delay 25 seconds
@21:28:45.83 ARV.dial :PP3
@21:28:46.25 Adding PP3
@21:28:46.26 delay 2 seconds
@21:28:48.34 AS.seek 0.53ml
@21:28:55.01 delay 10 seconds
@21:29:05.04 ARV.dial :air2
@21:29:05.48 adding ARV air
@21:29:05.49 delay 2 seconds
@21:29:07.57 AS.seek 0.536ml
@21:29:14.08 delay 5 seconds
@21:29:19.10 ARV.dial :carrier
@21:29:20.48 delay 7 seconds
@21:29:27.55 AS.seek 0.594ml
@21:30:26.48 delay 5 seconds
@21:30:31.50 ATV.dial :extSrc
@21:30:32.11 delay 2 seconds
@21:30:34.18 AS.seek 0.6ml
@21:30:40.50 delay 4 seconds
@21:30:44.52 ATV.dial ARV
@21:30:45.09 delay 2 seconds
@21:30:47.11 ARV.dial :carrier
@21:30:47.12 delay 2 seconds
@21:30:49.20 AS.seek 0.65ml
@21:31:39.81 delay 4 seconds
@21:31:43.83 ATV.dial PCR
@21:31:44.64 ASV.dial :waste1
@21:31:44.65 ARV.dial :air1
@21:31:45.09 Positioning Slug by 0.324ml
@21:31:45.18 AS.seek 0.326ml
@21:37:11.51 delay 5 seconds
@21:37:16.53 ATV.dial ARV
@21:37:17.34 ASV.dial :PCRlock
@21:37:17.75 delay 5 seconds
@21:37:22.77 Running AALPP3 Profile58CIQ2 thermocycling
@21:37:22.79 <PCR> PCR.program Profile58CIQ2
@21:37:22.91 Profile58CIQ2 should complete in < 1:36:15
@21:37:22.92 PCR.run 2:05:18.75
@22:58:31.26 PCR.off
@22:58:31.83 <MFBPCR> Running AALPP3 MeltProfile58 thermocycling
@22:58:31.85 <PCR> PCR.program MeltProfile58
@22:58:31.95 MeltProfile58 should complete in < 12:33
@22:58:31.96 PCR.run 20:41.25
@23:06:22.73 PCR.off
@23:06:23.74 <MFBPCR> PCR for AALPP3 completed -- closed /var/log/esp/AALPP321may0421h.pcr
@23:06:23.81 Cleaning PCR
AFV.select :carrierSolution
@23:06:23.93 delay 2 seconds
@23:06:25.96 AS.reconfigure ASconfig
@23:06:26.01 AS.seek 0.5ml
@23:06:36.29 delay 1 second
@23:06:37.31 AFV.select :holdingCoil
@23:06:37.41 ASV.dial :waste1
@23:06:37.81 ATV.dial :air3
@23:06:39.16 AS.reconfigure ASslow1
@23:06:39.22 AS.seek 0.51ml
@23:06:41.53 delay 2 seconds
@23:06:43.55 ATV.dial :bleach
@23:06:44.19 AS.seek 0.81ml
@23:07:44.79 delay 3 seconds
@23:07:47.81 ATV.dial :air3
@23:07:48.40 delay 3 seconds
@23:07:51.47 AS.seek 0.835ml
@23:07:56.76 delay 3 seconds
@23:07:59.78 ATV.dial :bleach
@23:08:00.42 AS.seek 0.82ml
@23:08:03.75 delay 3 seconds
@23:08:06.77 ARV.dial :waste
@23:08:07.42 delay 3 seconds
@23:08:10.44 ATV.dial :ARV
@23:08:11.38 AS.reconfigure ASconfig
@23:08:11.44 AS.seek 0.47ml
@23:08:31.84 AFV.select :carrierSolution
@23:08:31.95 delay 2 seconds
@23:08:33.98 AS.seek 1ml
@23:09:04.76 delay 1 second
@23:09:05.78 AFV.select :holdingCoil
@23:09:05.88 delay 1 second
@23:09:06.91 AS.reconfigure ASfast
@23:09:06.96 AS.seek 0ml
@23:09:34.31 AFV.select :carrierSolution
@23:09:34.42 delay 2 seconds
@23:09:36.45 AS.reconfigure ASconfig
@23:09:36.50 AS.seek 1ml
@23:10:34.29 delay 1 second
@23:10:35.31 AFV.select :holdingCoil
@23:10:35.41 delay 1 second
@23:10:36.44 AS.reconfigure ASfast
@23:10:36.49 AS.seek 0ml
@23:11:03.81 AFV.select :carrierSolution
@23:11:03.91 delay 2 seconds
@23:11:05.94 AS.reconfigure ASconfig
@23:11:05.99 AS.seek 1ml
@23:12:03.73 delay 1 second
@23:12:04.75 AFV.select :holdingCoil
@23:12:04.85 delay 1 second
@23:12:05.88 AS.reconfigure ASfast
@23:12:05.93 AS.seek 0ml
@23:12:33.26 ARV.dial between carrier and air1
@23:12:33.78 ATV.dial :air3
@23:12:35.13 AS.reconfigure ASslow1
@23:12:35.19 AS.seek 0.01ml
@23:12:37.62 delay 2 seconds
@23:12:39.64 ATV.dial :bleach
@23:12:40.28 AS.seek 0.81ml
@23:15:21.54 delay 3 seconds
@23:15:24.56 ATV.dial :air3
@23:15:25.15 delay 3 seconds
@23:15:28.22 AS.seek 0.835ml
@23:15:33.59 delay 3 seconds
@23:15:36.61 ATV.dial :bleach
@23:15:37.25 AS.seek 0.82ml
@23:15:40.58 delay 3 seconds
@23:15:43.60 ATV.dial PCR
@23:15:44.01 AS.reconfigure ASconfig
@23:15:44.07 AS.seek 0ml
@23:16:31.48 PCR.program :bleachProfile
@23:16:31.54 PCR program should complete in < 2:45
@23:16:31.55 PCR.run 10:00
@23:19:27.73 PCR.off
@23:19:27.77 AFV.select :carrierSolution
@23:19:27.88 delay 2 seconds
@23:19:29.91 AS.seek 1ml
@23:20:27.65 delay 1 second
@23:20:28.67 AFV.select :holdingCoil
@23:20:28.77 delay 1 second
@23:20:29.80 AS.reconfigure ASfast
@23:20:29.85 AS.seek 0ml
@23:20:57.17 delay 3 seconds
@23:21:00.19 AFV.select :carrierSolution
@23:21:00.29 delay 2 seconds
@23:21:02.32 AS.reconfigure ASconfig
@23:21:02.38 AS.seek 1ml
@23:22:00.22 delay 1 second
@23:22:01.24 AFV.select :holdingCoil
@23:22:01.34 delay 1 second
@23:22:02.37 AS.reconfigure ASfast
@23:22:02.42 AS.seek 0ml
@23:22:29.78 delay 3 seconds
@23:22:32.80 AFV.select :carrierSolution
@23:22:32.90 delay 2 seconds
@23:22:34.92 AS.reconfigure ASconfig
@23:22:34.99 AS.seek 1ml
@23:23:32.73 delay 1 second
@23:23:33.75 AFV.select :holdingCoil
@23:23:33.85 delay 1 second
@23:23:34.88 AS.reconfigure ASfast
@23:23:34.93 AS.seek 0ml
@23:24:02.29 delay 3 seconds
@23:24:05.31 AFV.select :carrierSolution
@23:24:05.41 delay 2 seconds
@23:24:07.44 AS.reconfigure ASconfig
@23:24:07.50 AS.seek 1ml
@23:25:05.29 delay 1 second
@23:25:06.31 AFV.select :holdingCoil
@23:25:06.41 delay 1 second
@23:25:07.44 AS.reconfigure ASfast
@23:25:07.49 AS.seek 0ml
@23:25:34.83 delay 3 seconds
@23:25:37.85 AFV.select :carrierSolution
@23:25:37.95 delay 2 seconds
@23:25:39.97 AS.reconfigure ASconfig
@23:25:40.04 AS.seek 1ml
@23:26:37.83 delay 1 second
@23:26:38.85 AFV.select :holdingCoil
@23:26:38.95 delay 1 second
@23:26:39.98 AS.reconfigure ASnorm
@23:26:40.03 AS.seek 0ml
@23:27:30.72 <MAIN> AFV.select :carrierSolution
@23:27:30.83 delay 2 seconds
@23:27:32.86 AS.reconfigure ASconfig
@23:27:32.91 AS.seek 0.5ml
@23:28:01.97 delay 1 second
@23:28:02.99 AFV.select :holdingCoil
@23:28:03.09 ATV.dial :air1
@23:28:04.67 AS.reconfigure ASslow2
@23:28:04.73 AS.seek 0.506ml
@23:28:10.90 ATV.dial :extSrc
@23:28:12.67 AS.seek 0.512ml
@23:28:19.39 delay 5 seconds
@23:28:24.41 ATV.dial :air1
@23:28:26.22 AS.reconfigure ASslow1
@23:28:26.28 AS.seek 0.542ml
@23:28:32.59 ATV.dial :waste
@23:28:33.15 AS.reconfigure ASconfig
@23:28:33.21 AS.seek 0ml
@23:29:04.82 <MFBPCR> Setting up PCR for AALPP3 -- writing /var/log/esp/AALPP321may0423h.pcr
@23:29:04.84 Building PCR Slug from extSrc with MM2 mix, PP3 primer
@23:29:04.87 Priming with carrier
@23:29:04.89 ATV.dial ARV
@23:29:06.47 ARV.dial :carrier
@23:29:06.81 AS.reconfigure ASnorm
@23:29:06.87 AS.seek 0.5ml
@23:29:32.48 AFV.select :carrierSolution
@23:29:32.60 delay 2 seconds
@23:29:34.63 AS.reconfigure ASconfig
@23:29:34.69 AS.seek 1ml
@23:30:03.73 delay 1 second
@23:30:04.75 AFV.select :holdingCoil
@23:30:04.85 delay 1 second
@23:30:05.87 ATV.dial :waste
@23:30:08.02 AS.reconfigure ASnorm
@23:30:08.08 AS.seek 0ml
@23:30:58.76 AFV.select :carrierSolution
@23:30:58.88 delay 2 seconds
@23:31:00.91 AS.reconfigure ASconfig
@23:31:00.96 AS.seek 0.5ml
@23:31:30.03 delay 1 second
@23:31:31.05 AFV.select :holdingCoil
@23:31:31.15 Building Slug
@23:31:31.16 ATV.dial :ARV
@23:31:32.92 delay 2 seconds
@23:31:34.94 ARV.dial :air1
@23:31:35.35 delay 2 seconds
@23:31:37.42 AS.reconfigure ASslow2
@23:31:37.48 AS.seek 0.506ml
@23:31:43.90 delay 2 seconds
@23:31:45.92 ARV.dial :MM2
@23:31:47.53 delay 2 seconds
@23:31:49.55 Adding MM2
@23:31:49.63 AS.seek 0.524ml
@23:32:08.06 delay 25 seconds
@23:32:33.08 ARV.dial :PP3
@23:32:33.48 Adding PP3
@23:32:33.49 delay 2 seconds
@23:32:35.57 AS.seek 0.53ml
@23:32:42.09 delay 10 seconds
@23:32:52.12 ARV.dial :air2
@23:32:52.56 adding ARV air
@23:32:52.57 delay 2 seconds
@23:32:54.65 AS.seek 0.536ml
@23:33:01.36 delay 5 seconds
@23:33:06.38 ARV.dial :carrier
@23:33:07.76 delay 7 seconds
@23:33:14.84 AS.seek 0.594ml
@23:34:13.39 delay 5 seconds
@23:34:18.41 ATV.dial :extSrc
@23:34:19.01 delay 2 seconds
@23:34:21.08 AS.seek 0.6ml
@23:34:27.26 delay 4 seconds
@23:34:31.28 ATV.dial ARV
@23:34:31.87 delay 2 seconds
@23:34:33.89 ARV.dial :carrier
@23:34:33.90 delay 2 seconds
@23:34:35.98 AS.seek 0.65ml
@23:35:26.47 delay 4 seconds
@23:35:30.49 ATV.dial PCR
@23:35:31.31 ASV.dial :waste1
@23:35:31.32 ARV.dial :air1
@23:35:31.76 Positioning Slug by 0.324ml
@23:35:31.85 AS.seek 0.326ml
@23:40:58.28 delay 5 seconds
@23:41:03.30 ATV.dial ARV
@23:41:04.12 ASV.dial :PCRlock
@23:41:04.51 delay 5 seconds
@23:41:09.53 Running AALPP3 Profile58CIQ2 thermocycling
@23:41:09.54 <PCR> PCR.program Profile58CIQ2
@23:41:09.66 Profile58CIQ2 should complete in < 1:36:15
@23:41:09.67 PCR.run 2:05:18.75
@01:02:15.45CEST05-May-21 PCR.off
@01:02:16.03 <MFBPCR> Running AALPP3 MeltProfile58 thermocycling
@01:02:16.04 <PCR> PCR.program MeltProfile58
@01:02:16.15 MeltProfile58 should complete in < 12:33
@01:02:16.16 PCR.run 20:41.25
@01:09:54.88 PCR.off
@01:09:55.87 <MFBPCR> PCR for AALPP3 completed -- closed /var/log/esp/AALPP321may0423h.pcr
@01:09:55.94 Cleaning PCR
@01:09:55.96 AFV.select :carrierSolution
@01:09:56.07 delay 2 seconds
@01:09:58.10 AS.reconfigure ASconfig
@01:09:58.15 AS.seek 0.5ml
@01:10:08.43 delay 1 second
@01:10:09.45 AFV.select :holdingCoil
@01:10:09.55 ASV.dial :waste1
@01:10:09.95 ATV.dial :air3
@01:10:11.30 AS.reconfigure ASslow1
@01:10:11.36 AS.seek 0.51ml
@01:10:13.68 delay 2 seconds
@01:10:15.70 ATV.dial :bleach
@01:10:16.34 AS.seek 0.81ml
@01:11:17.04 delay 3 seconds
@01:11:20.06 ATV.dial :air3
@01:11:20.65 delay 3 seconds
@01:11:23.72 AS.seek 0.835ml
@01:11:29.18 delay 3 seconds
@01:11:32.20 ATV.dial :bleach
@01:11:32.84 AS.seek 0.82ml
@01:11:36.15 delay 3 seconds
@01:11:39.17 ARV.dial :waste
@01:11:39.83 delay 3 seconds
@01:11:42.85 ATV.dial :ARV
@01:11:43.88 AS.reconfigure ASconfig
@01:11:44.31 AS.seek 0.47ml
@01:12:04.72 AFV.select :carrierSolution
@01:12:04.83 delay 2 seconds
@01:12:06.86 AS.seek 1ml
@01:12:37.59 delay 1 second
@01:12:38.61 AFV.select :holdingCoil
@01:12:38.71 delay 1 second
@01:12:39.74 AS.reconfigure ASfast
@01:12:39.79 AS.seek 0ml
@01:13:07.15 AFV.select :carrierSolution
@01:13:07.26 delay 2 seconds
@01:13:09.29 AS.reconfigure ASconfig
@01:13:09.34 AS.seek 1ml
@01:14:07.08 delay 1 second
@01:14:08.10 AFV.select :holdingCoil
@01:14:08.20 delay 1 second
@01:14:09.23 AS.reconfigure ASfast
@01:14:09.28 AS.seek 0ml
@01:14:36.62 AFV.select :carrierSolution
@01:14:36.73 delay 2 seconds
@01:14:38.76 AS.reconfigure ASconfig
@01:14:38.81 AS.seek 1ml
@01:15:36.54 delay 1 second
@01:15:37.56 AFV.select :holdingCoil
@01:15:37.66 delay 1 second
@01:15:38.69 AS.reconfigure ASfast
@01:15:38.74 AS.seek 0ml
@01:16:06.09 ARV.dial between carrier and air1
@01:16:06.62 ATV.dial :air3
@01:16:07.97 AS.reconfigure ASslow1
@01:16:08.03 AS.seek 0.01ml
@01:16:10.43 delay 2 seconds
@01:16:12.45 ATV.dial :bleach
@01:16:13.09 AS.seek 0.81ml
@01:18:54.37 delay 3 seconds
@01:18:57.39 ATV.dial :air3
@01:18:57.98 delay 3 seconds
@01:19:01.05 AS.seek 0.835ml
@01:19:06.37 delay 3 seconds
@01:19:09.39 ATV.dial :bleach
@01:19:10.03 AS.seek 0.82ml
@01:19:13.34 delay 3 seconds
@01:19:16.36 ATV.dial PCR
@01:19:16.79 AS.reconfigure ASconfig
@01:19:16.85 AS.seek 0ml
@01:20:04.26 PCR.program :bleachProfile
@01:20:04.33 PCR program should complete in < 2:45
@01:20:04.34 PCR.run 10:00
@01:23:00.13 PCR.off
@01:23:00.17 AFV.select :carrierSolution
@01:23:00.28 delay 2 seconds
@01:23:02.31 AS.seek 1ml
@01:24:00.09 delay 1 second
@01:24:01.11 AFV.select :holdingCoil
@01:24:01.21 delay 1 second
@01:24:02.23 AS.reconfigure ASfast
@01:24:02.29 AS.seek 0ml
@01:24:29.64 delay 3 seconds
@01:24:32.66 AFV.select :carrierSolution
@01:24:32.76 delay 2 seconds
@01:24:34.78 AS.reconfigure ASconfig
@01:24:34.89 AS.seek 1ml
@01:25:32.65 delay 1 second
@01:25:33.67 AFV.select :holdingCoil
@01:25:34.16 delay 1 second
@01:25:35.19 AS.reconfigure ASfast
@01:25:35.24 AS.seek 0ml
@01:26:02.59 delay 3 seconds
@01:26:05.61 AFV.select :carrierSolution
@01:26:05.71 delay 2 seconds
@01:26:07.74 AS.reconfigure ASconfig
@01:26:07.80 AS.seek 1ml
@01:27:05.59 delay 1 second
@01:27:06.61 AFV.select :holdingCoil
@01:27:06.71 delay 1 second
@01:27:07.74 AS.reconfigure ASfast
@01:27:07.79 AS.seek 0ml
@01:27:35.12 delay 3 seconds
@01:27:38.15 AFV.select :carrierSolution
@01:27:38.25 delay 2 seconds
@01:27:40.28 AS.reconfigure ASconfig
@01:27:40.34 AS.seek 1ml
@01:28:38.12 delay 1 second
@01:28:39.14 AFV.select :holdingCoil
@01:28:39.24 delay 1 second
@01:28:40.27 AS.reconfigure ASfast
@01:28:40.32 AS.seek 0ml
@01:29:07.67 delay 3 seconds
@01:29:10.69 AFV.select :carrierSolution
@01:29:10.79 delay 2 seconds
@01:29:12.81 AS.reconfigure ASconfig
@01:29:12.88 AS.seek 1ml
@01:30:10.68 delay 1 second
@01:30:11.70 AFV.select :holdingCoil
@01:30:11.80 delay 1 second
@01:30:12.83 AS.reconfigure ASnorm
@01:30:12.88 AS.seek 0ml
@01:31:03.56 <MAIN> AFV.select :carrierSolution
@01:31:03.66 delay 2 seconds
@01:31:05.69 AS.reconfigure ASconfig
@01:31:05.74 AS.seek 0.5ml
@01:31:34.79 delay 1 second
@01:31:35.81 AFV.select :holdingCoil
@01:31:35.94 ATV.dial :air1
@01:31:37.55 AS.reconfigure ASslow1
@01:31:37.61 AS.seek 0.52ml
@01:31:41.92 delay 5 seconds
@01:31:46.94 ATV.dial :extSrc
@01:31:48.85 AS.reconfigure ASslow2
@01:31:48.96 AS.seek 0.538ml
@01:32:07.25 delay 10 seconds
@01:32:17.28 ATV.dial :air1
@01:32:19.09 AS.reconfigure ASslow1
@01:32:19.15 AS.seek 0.568ml
@01:32:25.43 ATV.dial :waste
@01:32:26.00 AS.reconfigure ASconfig
@01:32:26.06 AS.seek 0ml
@01:32:59.15 <MFBPCR> Setting up PCR for AALPP3 -- writing /var/log/esp/AALPP321may0501h.pcr
@01:32:59.18 Building PCR Slug from extSrc with MM2 mix, PP3 primer
@01:32:59.20 Priming with carrier
@01:32:59.22 ATV.dial ARV
@01:33:00.79 ARV.dial :carrier
@01:33:01.13 AS.reconfigure ASnorm
@01:33:01.20 AS.seek 0.5ml
@01:33:26.75 AFV.select :carrierSolution
@01:33:26.86 delay 2 seconds
@01:33:28.89 AS.reconfigure ASconfig
@01:33:28.94 AS.seek 1ml
@01:33:57.95 delay 1 second
@01:33:58.97 AFV.select :holdingCoil
@01:33:59.07 delay 1 second
@01:34:00.09 ATV.dial :waste
@01:34:01.87 AS.reconfigure ASnorm
@01:34:01.93 AS.seek 0ml
@01:34:52.64 AFV.select :carrierSolution
@01:34:52.75 delay 2 seconds
@01:34:54.78 AS.reconfigure ASconfig
@01:34:54.83 AS.seek 0.5ml
@01:35:23.90 delay 1 second
@01:35:24.92 AFV.select :holdingCoil
@01:35:25.02 Building Slug
@01:35:25.04 ATV.dial :ARV
@01:35:26.79 delay 2 seconds
@01:35:28.81 ARV.dial :air1
@01:35:29.23 delay 2 seconds
@01:35:31.30 AS.reconfigure ASslow2
@01:35:31.36 AS.seek 0.506ml
@01:35:38.01 delay 2 seconds
@01:35:40.03 ARV.dial :MM2
@01:35:41.65 delay 2 seconds
@01:35:43.67 Adding MM2
@01:35:43.74 AS.seek 0.524ml
@01:36:01.92 delay 25 seconds
@01:36:26.94 ARV.dial :PP3
@01:36:27.36 Adding PP3
@01:36:27.37 delay 2 seconds
@01:36:29.45 AS.seek 0.53ml
@01:36:35.72 delay 10 seconds
@01:36:45.75 ARV.dial :air2
@01:36:46.19 adding ARV air
@01:36:46.20 delay 2 seconds
@01:36:48.28 AS.seek 0.536ml
@01:36:54.45 delay 5 seconds
@01:36:59.47 ARV.dial :carrier
@01:37:00.83 delay 7 seconds
@01:37:07.91 AS.seek 0.594ml
@01:38:06.36 delay 5 seconds
@01:38:11.38 ATV.dial :extSrc
@01:38:11.98 delay 2 seconds
@01:38:14.05 AS.seek 0.6ml
@01:38:20.69 delay 4 seconds
@01:38:24.71 ATV.dial ARV
@01:38:25.29 delay 2 seconds
@01:38:27.31 ARV.dial :carrier
@01:38:27.32 delay 2 seconds
@01:38:29.40 AS.seek 0.65ml
@01:39:20.45 delay 4 seconds
@01:39:24.47 ATV.dial PCR
@01:39:25.29 ASV.dial :waste1
@01:39:25.30 ARV.dial :air1
@01:39:25.73 Positioning Slug by 0.324ml
@01:39:25.82 AS.seek 0.326ml
@01:44:52.17 delay 5 seconds
@01:44:57.20 ATV.dial ARV
@01:44:58.42 ASV.dial :PCRlock
@01:44:58.83 delay 5 seconds
@01:45:03.85 Running AALPP3 Profile58CIQ2 thermocycling
@01:45:03.86 <PCR> PCR.program Profile58CIQ2
@01:45:03.98 Profile58CIQ2 should complete in < 1:36:15
@01:45:03.99 PCR.run 2:05:18.75
@03:06:16.69 PCR.off
@03:06:17.55 <MFBPCR> Running AALPP3 MeltProfile58 thermocycling
@03:06:17.56 <PCR> PCR.program MeltProfile58
@03:06:17.67 MeltProfile58 should complete in < 12:33
@03:06:17.68 PCR.run 20:41.25
@03:14:05.46 PCR.off
@03:14:06.46 <MFBPCR> PCR for AALPP3 completed -- closed /var/log/esp/AALPP321may0501h.pcr
@03:14:06.53 Cleaning PCR
@03:14:06.54 AFV.select :carrierSolution
@03:14:06.66 delay 2 seconds
@03:14:08.69 AS.reconfigure ASconfig
@03:14:08.74 AS.seek 0.5ml
@03:14:19.03 delay 1 second
@03:14:20.05 AFV.select :holdingCoil
@03:14:20.15 ASV.dial :waste1
@03:14:20.56 ATV.dial :air3
@03:14:21.91 AS.reconfigure ASslow1
@03:14:21.97 AS.seek 0.51ml
@03:14:24.29 delay 2 seconds
@03:14:26.31 ATV.dial :bleach
@03:14:26.95 AS.seek 0.81ml
@03:15:27.54 delay 3 seconds
@03:15:30.56 ATV.dial :air3
@03:15:31.15 delay 3 seconds
@03:15:34.22 AS.seek 0.835ml
@03:15:39.53 delay 3 seconds
@03:15:42.55 ATV.dial :bleach
@03:15:43.19 AS.seek 0.82ml
@03:15:46.48 delay 3 seconds
@03:15:49.50 ARV.dial :waste
@03:15:50.15 delay 3 seconds
@03:15:53.17 ATV.dial :ARV
@03:15:54.11 AS.reconfigure ASconfig
@03:15:54.17 AS.seek 0.47ml
@03:16:14.59 AFV.select :carrierSolution
@03:16:14.70 delay 2 seconds
@03:16:16.73 AS.seek 1ml
@03:16:47.51 delay 1 second
@03:16:48.53 AFV.select :holdingCoil
@03:16:48.63 delay 1 second
@03:16:49.66 AS.reconfigure ASfast
@03:16:49.71 AS.seek 0ml
@03:17:17.06 AFV.select :carrierSolution
@03:17:17.17 delay 2 seconds
@03:17:19.19 AS.reconfigure ASconfig
@03:17:19.25 AS.seek 1ml
@03:18:17.00 delay 1 second
@03:18:18.02 AFV.select :holdingCoil
@03:18:18.12 delay 1 second
@03:18:19.15 AS.reconfigure ASfast
@03:18:19.20 AS.seek 0ml
@03:18:46.53 AFV.select :carrierSolution
@03:18:46.64 delay 2 seconds
@03:18:48.67 AS.reconfigure ASconfig
@03:18:48.72 AS.seek 1ml
@03:19:46.47 delay 1 second
@03:19:47.49 AFV.select :holdingCoil
@03:19:47.59 delay 1 second
@03:19:48.62 AS.reconfigure ASfast
@03:19:48.67 AS.seek 0ml
@03:20:16.01 ARV.dial between carrier and air1
@03:20:16.51 ATV.dial :air3
@03:20:17.88 AS.reconfigure ASslow1
@03:20:17.94 AS.seek 0.01ml
@03:20:20.50 delay 2 seconds
@03:20:22.52 ATV.dial :bleach
@03:20:23.17 AS.seek 0.81ml
@03:23:04.31 delay 3 seconds
@03:23:07.33 ATV.dial :air3
@03:23:07.92 delay 3 seconds
@03:23:10.99 AS.seek 0.835ml
@03:23:16.30 delay 3 seconds
@03:23:19.32 ATV.dial :bleach
@03:23:19.95 AS.seek 0.82ml
@03:23:23.40 delay 3 seconds
@03:23:26.42 ATV.dial PCR
@03:23:26.84 AS.reconfigure ASconfig
@03:23:26.90 AS.seek 0ml
@03:24:14.31 PCR.program :bleachProfile
@03:24:14.38 PCR program should complete in < 2:45
@03:24:14.39 PCR.run 10:00
@03:27:10.60 PCR.off
@03:27:10.64 AFV.select :carrierSolution
@03:27:10.75 delay 2 seconds
@03:27:12.78 AS.seek 1ml
@03:28:10.53 delay 1 second
@03:28:11.55 AFV.select :holdingCoil
@03:28:11.65 delay 1 second
@03:28:12.68 AS.reconfigure ASfast
@03:28:12.73 AS.seek 0ml
@03:28:40.06 delay 3 seconds
@03:28:43.08 AFV.select :carrierSolution
@03:28:43.18 delay 2 seconds
@03:28:45.21 AS.reconfigure ASconfig
@03:28:45.27 AS.seek 1ml
@03:29:43.01 delay 1 second
@03:29:44.03 AFV.select :holdingCoil
@03:29:44.13 delay 1 second
@03:29:45.16 AS.reconfigure ASfast
@03:29:45.21 AS.seek 0ml
@03:30:12.67 delay 3 seconds
@03:30:15.69 AFV.select :carrierSolution
@03:30:15.79 delay 2 seconds
@03:30:17.82 AS.reconfigure ASconfig
@03:30:17.88 AS.seek 1ml
@03:31:15.65 delay 1 second
@03:31:16.67 AFV.select :holdingCoil
@03:31:16.77 delay 1 second
@03:31:17.80 AS.reconfigure ASfast
@03:31:17.85 AS.seek 0ml
@03:31:45.19 delay 3 seconds
@03:31:48.21 AFV.select :carrierSolution
@03:31:48.31 delay 2 seconds
@03:31:50.33 AS.reconfigure ASconfig
@03:31:50.40 AS.seek 1ml
@03:32:48.15 delay 1 second
@03:32:49.17 AFV.select :holdingCoil
@03:32:49.27 delay 1 second
@03:32:50.30 AS.reconfigure ASfast
@03:32:50.35 AS.seek 0ml
@03:33:17.70 delay 3 seconds
@03:33:20.72 AFV.select :carrierSolution
@03:33:20.82 delay 2 seconds
@03:33:22.84 AS.reconfigure ASconfig
@03:33:22.91 AS.seek 1ml
@03:34:20.65 delay 1 second
@03:34:21.67 AFV.select :holdingCoil
@03:34:21.77 delay 1 second
@03:34:22.80 AS.reconfigure ASnorm
@03:34:22.85 AS.seek 0ml
@03:35:13.56 <MAIN> AFV.select :carrierSolution
@03:35:13.66 delay 2 seconds
@03:35:15.69 AS.reconfigure ASconfig
@03:35:15.74 AS.seek 0.5ml
@03:35:44.81 delay 1 second
@03:35:45.83 AFV.select :holdingCoil
@03:35:45.94 ATV.dial :air1
@03:35:47.52 AS.reconfigure ASslow2
@03:35:47.58 AS.seek 0.506ml
@03:35:54.12 ATV.dial :extSrc
@03:35:55.91 AS.seek 0.512ml
@03:36:02.26 delay 5 seconds
@03:36:07.28 ATV.dial :air1
@03:36:09.15 AS.reconfigure ASslow1
@03:36:09.24 AS.seek 0.542ml
@03:36:15.59 ATV.dial :waste
@03:36:16.17 AS.reconfigure ASconfig
@03:36:16.23 AS.seek 0ml
@03:36:47.86 <MFBPCR> Setting up PCR for AALPP3 -- writing /var/log/esp/AALPP321may0503h.pcr
@03:36:47.89 Building PCR Slug from extSrc with MM2 mix, PP3 primer
@03:36:47.91 Priming with carrier
@03:36:47.93 ATV.dial ARV
@03:36:50.62 ARV.dial :carrier
@03:36:51.07 AS.reconfigure ASnorm
@03:36:51.18 AS.seek 0.5ml
@03:37:16.79 AFV.select :carrierSolution
@03:37:16.91 delay 2 seconds
@03:37:18.94 AS.reconfigure ASconfig
@03:37:19.00 AS.seek 1ml
@03:37:48.01 delay 1 second
@03:37:49.03 AFV.select :holdingCoil
@03:37:49.13 delay 1 second
@03:37:50.15 ATV.dial :waste
@03:37:51.90 AS.reconfigure ASnorm
@03:37:51.96 AS.seek 0ml
@03:38:42.70 AFV.select :carrierSolution
@03:38:42.82 delay 2 seconds
@03:38:44.85 AS.reconfigure ASconfig
@03:38:44.90 AS.seek 0.5ml
@03:39:13.94 delay 1 second
@03:39:14.96 AFV.select :holdingCoil
@03:39:15.06 Building Slug
@03:39:15.07 ATV.dial :ARV
@03:39:16.83 delay 2 seconds
@03:39:18.85 ARV.dial :air1
@03:39:19.27 delay 2 seconds
@03:39:21.34 AS.reconfigure ASslow2
@03:39:21.40 AS.seek 0.506ml
@03:39:27.92 delay 2 seconds
@03:39:29.94 ARV.dial :MM2
@03:39:31.54 delay 2 seconds
@03:39:33.56 Adding MM2
@03:39:33.63 AS.seek 0.524ml
@03:39:52.26 delay 25 seconds
@03:40:17.28 ARV.dial :PP3
@03:40:17.70 Adding PP3
@03:40:17.71 delay 2 seconds
@03:40:19.79 AS.seek 0.53ml
@03:40:26.58 delay 10 seconds
@03:40:36.61 ARV.dial :air2
@03:40:37.04 adding ARV air
@03:40:37.05 delay 2 seconds
@03:40:39.13 AS.seek 0.536ml
@03:40:45.72 delay 5 seconds
@03:40:50.74 ARV.dial :carrier
@03:40:52.11 delay 7 seconds
@03:40:59.19 AS.seek 0.594ml
@03:41:58.11 delay 5 seconds
@03:42:03.13 ATV.dial :extSrc
@03:42:03.73 delay 2 seconds
@03:42:05.80 AS.seek 0.6ml
@03:42:12.08 delay 4 seconds
@03:42:16.10 ATV.dial ARV
@03:42:16.69 delay 2 seconds
@03:42:18.71 ARV.dial :carrier
@03:42:18.72 delay 2 seconds
@03:42:20.80 AS.seek 0.65ml
@03:43:11.23 delay 4 seconds
@03:43:15.25 ATV.dial PCR
@03:43:16.08 ASV.dial :waste1
@03:43:16.09 ARV.dial :air1
@03:43:16.51 Positioning Slug by 0.324ml
@03:43:16.60 AS.seek 0.326ml
@03:48:42.39 delay 5 seconds
@03:48:47.41 ATV.dial ARV
@03:48:48.23 ASV.dial :PCRlock
@03:48:48.64 delay 5 seconds
@03:48:53.66 Running AALPP3 Profile58CIQ2 thermocycling
@03:48:53.67 <PCR> PCR.program Profile58CIQ2
@03:48:53.79 Profile58CIQ2 should complete in < 1:36:15
@03:48:53.80 PCR.run 2:05:18.75
@05:09:59.42 PCR.off
@05:10:00.00 <MFBPCR> Running AALPP3 MeltProfile58 thermocycling
@05:10:00.01 <PCR> PCR.program MeltProfile58
@05:10:00.11 MeltProfile58 should complete in < 12:33
@05:10:00.12 PCR.run 20:41.25
@05:17:38.82 PCR.off
@05:17:39.81 <MFBPCR> PCR for AALPP3 completed -- closed /var/log/esp/AALPP321may0503h.pcr
@05:17:39.88 Cleaning PCR
@05:17:39.89 AFV.select :carrierSolution
@05:17:40.01 delay 2 seconds
@05:17:42.04 AS.reconfigure ASconfig
@05:17:42.09 AS.seek 0.5ml
@05:17:52.39 delay 1 second
@05:17:53.41 AFV.select :holdingCoil
@05:17:53.51 ASV.dial :waste1
@05:17:53.92 ATV.dial :air3
@05:17:55.25 AS.reconfigure ASslow1
@05:17:55.31 AS.seek 0.51ml
@05:17:57.61 delay 2 seconds
@05:17:59.63 ATV.dial :bleach
@05:18:00.27 AS.seek 0.81ml
@05:19:00.87 delay 3 seconds
@05:19:03.89 ATV.dial :air3
@05:19:04.53 delay 3 seconds
@05:19:07.60 AS.seek 0.835ml
@05:19:12.99 delay 3 seconds
@05:19:16.01 ATV.dial :bleach
@05:19:16.65 AS.seek 0.82ml
@05:19:20.05 delay 3 seconds
@05:19:23.07 ARV.dial :waste
@05:19:23.70 delay 3 seconds
@05:19:26.72 ATV.dial :ARV
@05:19:27.66 AS.reconfigure ASconfig
@05:19:27.72 AS.seek 0.47ml
@05:19:48.14 AFV.select :carrierSolution
@05:19:48.25 delay 2 seconds
@05:19:50.28 AS.seek 1ml
@05:20:21.01 delay 1 second
@05:20:22.03 AFV.select :holdingCoil
@05:20:22.13 delay 1 second
@05:20:23.16 AS.reconfigure ASfast
@05:20:23.21 AS.seek 0ml
@05:20:50.55 AFV.select :carrierSolution
@05:20:50.66 delay 2 seconds
@05:20:52.69 AS.reconfigure ASconfig
@05:20:52.74 AS.seek 1ml
@05:21:50.48 delay 1 second
@05:21:51.50 AFV.select :holdingCoil
@05:21:51.60 delay 1 second
@05:21:52.63 AS.reconfigure ASfast
@05:21:52.68 AS.seek 0ml
@05:22:20.00 AFV.select :carrierSolution
@05:22:20.11 delay 2 seconds
@05:22:22.14 AS.reconfigure ASconfig
@05:22:22.19 AS.seek 1ml
@05:23:19.95 delay 1 second
@05:23:20.97 AFV.select :holdingCoil
@05:23:21.07 delay 1 second
@05:23:22.10 AS.reconfigure ASfast
@05:23:22.15 AS.seek 0ml
@05:23:49.48 ARV.dial between carrier and air1
@05:23:50.00 ATV.dial :air3
@05:23:51.36 AS.reconfigure ASslow1
@05:23:51.42 AS.seek 0.01ml
@05:23:53.98 delay 2 seconds
@05:23:56.00 ATV.dial :bleach
@05:23:56.66 AS.seek 0.81ml
@05:26:37.81 delay 3 seconds
@05:26:40.83 ATV.dial :air3
@05:26:41.42 delay 3 seconds
@05:26:44.49 AS.seek 0.835ml
@05:26:49.77 delay 3 seconds
@05:26:52.79 ATV.dial :bleach
@05:26:53.42 AS.seek 0.82ml
@05:26:56.75 delay 3 seconds
@05:26:59.77 ATV.dial PCR
@05:27:00.19 AS.reconfigure ASconfig
@05:27:00.25 AS.seek 0ml
@05:27:47.67 PCR.program :bleachProfile
@05:27:47.73 PCR program should complete in < 2:45
@05:27:47.74 PCR.run 10:00
@05:30:43.86 PCR.off
@05:30:43.96 AFV.select :carrierSolution
@05:30:44.07 delay 2 seconds
@05:30:46.10 AS.seek 1ml
@05:31:43.84 delay 1 second
@05:31:44.86 AFV.select :holdingCoil
@05:31:44.96 delay 1 second
@05:31:45.99 AS.reconfigure ASfast
@05:31:46.04 AS.seek 0ml
@05:32:13.39 delay 3 seconds
@05:32:16.41 AFV.select :carrierSolution
@05:32:16.51 delay 2 seconds
@05:32:18.54 AS.reconfigure ASconfig
@05:32:18.59 AS.seek 1ml
@05:33:16.37 delay 1 second
@05:33:17.39 AFV.select :holdingCoil
@05:33:17.49 delay 1 second
@05:33:18.52 AS.reconfigure ASfast
@05:33:18.57 AS.seek 0ml
@05:33:45.94 delay 3 seconds
@05:33:48.96 AFV.select :carrierSolution
@05:33:49.06 delay 2 seconds
@05:33:51.09 AS.reconfigure ASconfig
@05:33:51.14 AS.seek 1ml
@05:34:48.89 delay 1 second
@05:34:49.91 AFV.select :holdingCoil
@05:34:50.01 delay 1 second
@05:34:51.04 AS.reconfigure ASfast
@05:34:51.09 AS.seek 0ml
@05:35:18.44 delay 3 seconds
@05:35:21.46 AFV.select :carrierSolution
@05:35:21.56 delay 2 seconds
@05:35:23.59 AS.reconfigure ASconfig
@05:35:23.64 AS.seek 1ml
@05:36:21.39 delay 1 second
@05:36:22.41 AFV.select :holdingCoil
@05:36:22.51 delay 1 second
@05:36:23.54 AS.reconfigure ASfast
@05:36:23.59 AS.seek 0ml
@05:36:50.95 delay 3 seconds
@05:36:53.97 AFV.select :carrierSolution
@05:36:54.07 delay 2 seconds
@05:36:56.10 AS.reconfigure ASconfig
@05:36:56.16 AS.seek 1ml
@05:37:53.90 delay 1 second
@05:37:54.92 AFV.select :holdingCoil
@05:37:55.02 delay 1 second
@05:37:56.05 AS.reconfigure ASnorm
@05:37:56.10 AS.seek 0ml
@05:38:46.82 <MAIN> deconEluateRecoveryLine extSrc
@05:38:46.83 ATV.dial :waste
@05:38:48.14 AS.seek 0ml
@05:38:48.22 ATV.dial :air3
@05:38:49.10 AS.reconfigure ASslow1
@05:38:49.16 AS.seek 0.01ml
@05:38:51.78 delay 2 seconds
@05:38:53.80 ATV.dial :bleach
@05:38:54.27 AS.seek 0.31ml
@05:39:54.90 delay 3 seconds
@05:39:57.92 ATV.dial :air3
@05:39:58.51 delay 3 seconds
@05:40:01.58 AS.seek 0.335ml
@05:40:06.95 delay 3 seconds
@05:40:09.97 ATV.dial :bleach
@05:40:10.61 AS.seek 0.32ml
@05:40:14.01 delay 3 seconds
@05:40:17.03 ATV.dial :extSrc
@05:40:17.69 AS.seek 0ml
@05:41:22.37 AFV.select :carrierSolution
@05:41:22.48 delay 2 seconds
@05:41:24.51 AS.reconfigure ASconfig
@05:41:24.56 AS.seek 0.5ml
@05:41:53.61 delay 1 second
@05:41:54.63 AFV.select :holdingCoil
@05:41:54.73 ATV.dial :extSrc
@05:41:54.75 AS.reconfigure ASslow1
@05:41:54.81 AS.seek 0ml
@05:43:35.76 AFV.select :carrierSolution
@05:43:35.87 delay 2 seconds
@05:43:37.90 AS.reconfigure ASconfig
@05:43:37.95 AS.seek 0.5ml
@05:44:06.98 delay 1 second
@05:44:08.00 AFV.select :holdingCoil
@05:44:08.10 ATV.dial :extSrc
@05:44:08.12 AS.reconfigure ASslow1
@05:44:08.18 AS.seek 0ml
@05:45:49.01 AFV.select :carrierSolution
@05:45:49.12 delay 2 seconds
@05:45:51.15 AS.reconfigure ASconfig
@05:45:51.20 AS.seek 0.5ml
@05:46:20.25 delay 1 second
@05:46:21.27 AFV.select :holdingCoil
@05:46:21.37 ATV.dial :extSrc
@05:46:21.39 AS.reconfigure ASslow1
@05:46:21.46 AS.seek 0ml
@05:48:02.42 AFV.select :carrierSolution
@05:48:02.53 delay 2 seconds
@05:48:04.56 AS.reconfigure ASconfig
@05:48:04.61 AS.seek 0.5ml
@05:48:33.64 delay 1 second
@05:48:34.66 AFV.select :holdingCoil
@05:48:34.76 ATV.dial :extSrc
@05:48:34.78 AS.reconfigure ASslow1
@05:48:34.84 AS.seek 0ml
@05:50:15.67 ATV.dial :air2
@05:50:17.06 AS.reconfigure ASslow0
@05:50:17.12 AS.seek 0.5ml
@05:51:07.72 ATV.dial :extSrc
@05:51:09.29 AS.seek 0.02ml
@05:51:57.90 delay 1 second
@05:51:58.92 ATV.dial :waste
@05:52:00.28 AS.reconfigure ASconfig
@05:52:00.34 AS.seek 0ml
@05:52:01.78 deconEluateRecoveryLine extSrc
@05:52:01.79 ATV.dial :waste
@05:52:01.82 AS.reconfigure ASnorm
@05:52:01.88 AS.seek 0ml
@05:52:02.00 ATV.dial :air3
@05:52:02.88 AS.reconfigure ASslow1
@05:52:02.95 AS.seek 0.01ml
@05:52:05.36 delay 2 seconds
@05:52:07.38 ATV.dial :bleach
@05:52:07.85 AS.seek 0.31ml
@05:53:08.45 delay 3 seconds
@05:53:11.47 ATV.dial :air3
@05:53:12.06 delay 3 seconds
@05:53:15.13 AS.seek 0.335ml
@05:53:20.48 delay 3 seconds
@05:53:23.50 ATV.dial :bleach
@05:53:24.14 AS.seek 0.32ml
@05:53:27.47 delay 3 seconds
@05:53:30.49 ATV.dial :extSrc
@05:53:31.56 AS.seek 0ml
@05:54:36.19 AFV.select :carrierSolution
@05:54:36.30 delay 2 seconds
@05:54:38.33 AS.reconfigure ASconfig
@05:54:38.38 AS.seek 0.5ml
@05:55:07.42 delay 1 second
@05:55:08.44 AFV.select :holdingCoil
@05:55:08.54 ATV.dial :extSrc
@05:55:08.56 AS.reconfigure ASslow1
@05:55:08.63 AS.seek 0ml
@05:56:49.48 AFV.select :carrierSolution
@05:56:49.59 delay 2 seconds
@05:56:51.62 AS.reconfigure ASconfig
@05:56:51.67 AS.seek 0.5ml
@05:57:20.72 delay 1 second
@05:57:21.74 AFV.select :holdingCoil
@05:57:21.84 ATV.dial :extSrc
@05:57:21.86 AS.reconfigure ASslow1
@05:57:21.92 AS.seek 0ml
@05:59:02.76 AFV.select :carrierSolution
@05:59:02.87 delay 2 seconds
@05:59:04.90 AS.reconfigure ASconfig
@05:59:04.95 AS.seek 0.5ml
@05:59:34.00 delay 1 second
@05:59:35.02 AFV.select :holdingCoil
@05:59:35.12 ATV.dial :extSrc
@05:59:35.14 AS.reconfigure ASslow1
@05:59:35.20 AS.seek 0ml
@06:01:16.05 AFV.select :carrierSolution
@06:01:16.16 delay 2 seconds
@06:01:18.19 AS.reconfigure ASconfig
@06:01:18.24 AS.seek 0.5ml
@06:01:47.28 delay 1 second
@06:01:48.30 AFV.select :holdingCoil
@06:01:48.40 ATV.dial :extSrc
@06:01:48.42 AS.reconfigure ASslow1
@06:01:48.49 AS.seek 0ml
@06:03:29.33 ATV.dial :air2
@06:03:30.72 AS.reconfigure ASslow0
@06:03:30.78 AS.seek 0.5ml
@06:04:21.42 ATV.dial :extSrc
@06:04:23.00 AS.seek 0.02ml
@06:05:11.61 delay 1 second
@06:05:12.63 ATV.dial :waste
@06:05:13.98 AS.reconfigure ASconfig
@06:05:14.04 AS.seek 0ml
@06:05:15.50 deconEluateRecoveryLine extSrc
@06:05:15.51 ATV.dial :waste
@06:05:15.54 AS.reconfigure ASnorm
@06:05:15.60 AS.seek 0ml
@06:05:15.70 ATV.dial :air3
@06:05:16.58 AS.reconfigure ASslow1
@06:05:16.65 AS.seek 0.01ml
@06:05:19.08 delay 2 seconds
@06:05:21.10 ATV.dial :bleach
@06:05:21.56 AS.seek 0.31ml
@06:06:22.17 delay 3 seconds
@06:06:25.19 ATV.dial :air3
@06:06:25.78 delay 3 seconds
@06:06:28.85 AS.seek 0.335ml
@06:06:34.24 delay 3 seconds
@06:06:37.26 ATV.dial :bleach
@06:06:37.89 AS.seek 0.32ml
@06:06:41.15 delay 3 seconds
@06:06:44.17 ATV.dial :extSrc
@06:06:44.83 AS.seek 0ml
@06:07:49.47 AFV.select :carrierSolution
@06:07:49.58 delay 2 seconds
@06:07:51.61 AS.reconfigure ASconfig
@06:07:51.66 AS.seek 0.5ml
@06:08:20.70 delay 1 second
@06:08:21.72 AFV.select :holdingCoil
@06:08:21.82 ATV.dial :extSrc
@06:08:21.84 AS.reconfigure ASslow1
@06:08:21.90 AS.seek 0ml
@06:10:02.75 AFV.select :carrierSolution
@06:10:02.86 delay 2 seconds
@06:10:04.89 AS.reconfigure ASconfig
@06:10:04.94 AS.seek 0.5ml
@06:10:33.97 delay 1 second
@06:10:34.98 AFV.select :holdingCoil
@06:10:35.08 ATV.dial :extSrc
@06:10:35.10 AS.reconfigure ASslow1
@06:10:35.16 AS.seek 0ml
@06:12:16.01 AFV.select :carrierSolution
@06:12:16.12 delay 2 seconds
@06:12:18.15 AS.reconfigure ASconfig
@06:12:18.20 AS.seek 0.5ml
@06:12:47.25 delay 1 second
@06:12:48.27 AFV.select :holdingCoil
@06:12:48.37 ATV.dial :extSrc
@06:12:48.39 AS.reconfigure ASslow1
@06:12:48.45 AS.seek 0ml
@06:14:29.28 AFV.select :carrierSolution
@06:14:29.39 delay 2 seconds
@06:14:31.42 AS.reconfigure ASconfig
@06:14:31.47 AS.seek 0.5ml
@06:15:00.50 delay 1 second
@06:15:01.52 AFV.select :holdingCoil
@06:15:01.62 ATV.dial :extSrc
@06:15:01.64 AS.reconfigure ASslow1
@06:15:01.70 AS.seek 0ml
@06:16:42.70 ATV.dial :air2
@06:16:44.11 AS.reconfigure ASslow0
@06:16:44.17 AS.seek 0.5ml
@06:17:34.78 ATV.dial :extSrc
@06:17:36.35 AS.seek 0.02ml
@06:18:24.94 delay 1 second
@06:18:25.96 ATV.dial :waste
@06:18:27.31 AS.reconfigure ASconfig
@06:18:27.37 AS.seek 0ml
@06:18:28.85 <mwj> Session Ended
@13:33:48.53CEST05-May-21 <MAIN> >RESET< ESP2 v3.30 I2C Gateway v3.06 for core bus
@13:33:51.09 
Configuration for mer 7/20/20 brent@mbari.org
/home/esp/esp2/type/shallow/mer/configure.rb
@13:33:51.18 
Configuration for MFB3 by preston@mbari.org
/home/esp/esp2/type/mfb/MFB3/configure.rb
@13:33:56.89 Polling Can every 10:00
@13:33:57.03 Cleanly exited at 06:18:28CEST05-May-21
@13:33:57.06 Loading /home/esp/esp2/mission/stdMER2017.rb mission
@13:33:57.10 Powering core bus
@13:33:57.95 <mwj> Session Begun
@13:34:02.19 <MAIN> Gate.power :raw,:ON
@13:34:02.70 CS.configure :default
@13:34:02.80 PS.configure :default
@13:34:02.89 SS.configure :default
@13:34:02.99 CC.configure :default
@13:34:03.08 PC.configure :default
@13:34:03.20 Forearm.configure :loose
@13:34:03.29 Elbow.configure :loose
@13:34:03.40 SC.configure :loose
@13:34:03.49 SE.configure :default
@13:34:03.54 CH.coast
@13:34:03.60 CH.configure :default
@13:34:03.64 PH.coast
@13:34:03.70 PH.configure :default
@13:34:03.81 SPE.coast
@13:34:03.86 SPE.configure SPEconfig
@13:34:03.95 AS.configure ASconfig
@13:34:04.01 PCR.off
@13:34:04.05 PCR.pidtemp= 50,0.1,0,300,25,25
@13:34:04.09 PCR.caltemp= 2.5051,0.0372,0
@13:34:04.13 All dwarf controllers running firmware version 3.75
@13:34:04.35 <rdySampler> Intake.select :close
@13:34:04.39 Exhaust.select :close
@13:34:04.41 CTV.dial :air
@13:34:04.51 <rdyProcessing> PSV.dial :air
@13:34:04.65 PS.reconfigure :default
@13:34:04.67 <rdySampler> CSV.dial :waste1
@13:34:04.70 <MAIN> Forearm.reconfigure :loose
@13:34:04.86 <rdyProcessing> PS.home.seek 0ml
@13:34:04.89 <MAIN> Forearm.home.seek :retract
@13:34:04.97 <rdySampler> SSV.dial :spit,avoiding: :exhaust
@13:34:05.11 <readyCS> CS.reconfigure :fast
@13:34:05.15 <rdySampler> SS.reconfigure :default
@13:34:05.23 <readyCS> CS.home.seek 0ml
@13:34:05.27 <rdySampler> SS.home.seek 0ml
@13:34:05.69 <closeHand> Hand.close
@13:34:05.72 <MAIN> Elbow.jog 1500
@13:34:06.33 Elbow.reconfigure :loose
@13:34:06.39 Elbow.home.seek :clear
@13:34:11.05 SE.reconfigure :default
@13:34:11.10 SE.home.seek :down
@13:34:11.75 SC.reconfigure :sloppy
@13:34:11.80 SC.home.seek :initial
@13:34:14.21 <rdyProcessing> PS.coast
@13:34:24.95 <MAIN> Forearm.seek :retract
@13:34:25.02 Elbow.seek :hammer
@13:34:26.24 Forearm.reconfigure :tight
@13:34:26.31 Forearm.seek retracted
@13:34:26.54 <openPC> PC.reconfigure :default
@13:34:26.57 <MAIN> CC.reconfigure :default
@13:34:26.65 <openPC> PC.home.seek :open
@13:34:26.68 <MAIN> CC.home.seek :open
@13:34:29.67 Readying the MFB
@13:34:29.69 Homing MFB Syringe
@13:34:29.70 AFV.select :holdingCoil
@13:34:29.86 ATV.dial :air2
@13:34:30.97 AS.reconfigure ASconfig
@13:34:31.02 AS.home.seek :home
@13:34:36.52 AFV.select :carrierSolution
@13:34:37.01 delay 2 seconds
@13:34:39.03 AS.seek 1ml
@13:35:31.91 delay 1 second
@13:35:32.94 AFV.select :holdingCoil
@13:35:33.04 delay 1 second
@13:35:34.06 ATV.dial :waste
@13:35:35.36 AS.reconfigure ASnorm
@13:35:35.41 AS.home.seek 0ml
@13:36:27.92 ATV.dial :waste
@13:36:27.94 ARV.dial between carrier and air1
@13:36:28.04 ASV.dial :waste1
@13:36:28.11 AFV.select :carrierSolution
@13:36:28.23 delay 2 seconds
@13:36:30.25 AS.reconfigure ASconfig
@13:36:30.31 AS.seek 0.5ml
@13:36:59.35 delay 1 second
@13:37:00.38 AFV.select :holdingCoil
@13:37:00.49 ATV.dial :extSrc
@13:37:01.89 AS.reconfigure ASslow1
@13:37:01.95 AS.seek 0.53ml
@13:37:08.25 delay 5 seconds
@13:37:13.33 AS.reconfigure ASslow2
@13:37:13.39 AS.seek 0.54ml
@13:37:23.97 delay 20 seconds
@13:37:44.04 AS.reconfigure ASslow1
@13:37:44.10 AS.seek 0.57ml
@13:37:50.39 delay 5 seconds
@13:37:55.47 AS.reconfigure ASslow2
@13:37:55.53 AS.seek 0.58ml
@13:38:05.86 delay 20 seconds
@13:38:25.93 AS.reconfigure ASslow1
@13:38:25.99 AS.seek 0.61ml
@13:38:32.33 delay 5 seconds
@13:38:37.41 AS.reconfigure ASslow2
@13:38:37.47 AS.seek 0.62ml
@13:38:48.00 delay 20 seconds
@13:39:08.07 AS.reconfigure ASslow1
@13:39:08.13 AS.seek 0.65ml
@13:39:14.44 delay 5 seconds
@13:39:19.52 AS.reconfigure ASslow2
@13:39:19.58 AS.seek 0.66ml
@13:39:29.99 delay 20 seconds
@13:39:50.02 AS.reconfigure ASslow1
@13:39:50.07 AS.seek 0.705ml
@13:39:59.39 delay 10 seconds
@13:40:09.42 ATV.dial :air1
@13:40:11.23 AS.seek 0.725ml
@13:40:15.53 delay 3 seconds
@13:40:18.56 ATV.dial :waste
@13:40:19.15 AS.reconfigure ASslow0
@13:40:19.20 AS.seek 0ml
@13:41:32.58 <MFBPCR> Setting up PCR for ACOPP5 -- writing /var/log/esp/ACOPP521may0513h.pcr
@13:41:32.61 Building PCR Slug from extSrc with MM3 mix, PP5 primer
@13:41:32.63 Priming with carrier
@13:41:32.65 ATV.dial ARV
@13:41:34.22 ARV.dial :carrier
@13:41:34.58 AS.reconfigure ASnorm
@13:41:34.64 AS.seek 0.5ml
@13:42:00.21 AFV.select :carrierSolution
@13:42:00.34 delay 2 seconds
@13:42:02.36 AS.reconfigure ASconfig
@13:42:02.43 AS.seek 1ml
@13:42:31.44 delay 1 second
@13:42:32.47 AFV.select :holdingCoil
@13:42:32.57 delay 1 second
@13:42:33.59 ATV.dial :waste
@13:42:35.37 AS.reconfigure ASnorm
@13:42:35.43 AS.seek 0ml
@13:43:26.11 AFV.select :carrierSolution
@13:43:26.23 delay 2 seconds
@13:43:28.25 AS.reconfigure ASconfig
@13:43:28.31 AS.seek 0.5ml
@13:43:57.36 delay 1 second
@13:43:58.39 AFV.select :holdingCoil
@13:43:58.49 Building Slug
@13:43:58.50 ATV.dial :ARV
@13:44:00.26 delay 2 seconds
@13:44:02.28 ARV.dial :air1
@13:44:02.70 delay 2 seconds
@13:44:04.76 AS.reconfigure ASslow2
@13:44:04.83 AS.seek 0.506ml
@13:44:11.11 delay 2 seconds
@13:44:13.14 ARV.dial :MM3
@13:44:14.29 delay 2 seconds
@13:44:16.31 Adding MM3
@13:44:16.40 AS.seek 0.524ml
@13:44:34.83 delay 25 seconds
@13:44:59.86 ARV.dial :PP5
@13:45:00.28 Adding PP5
@13:45:00.29 delay 2 seconds
@13:45:02.37 AS.seek 0.53ml
@13:45:08.63 delay 10 seconds
@13:45:18.67 ARV.dial :air3
@13:45:19.09 adding ARV air
@13:45:19.10 delay 2 seconds
@13:45:21.18 AS.seek 0.536ml
@13:45:27.46 delay 5 seconds
@13:45:32.49 ARV.dial :carrier
@13:45:33.86 delay 7 seconds
@13:45:40.94 AS.seek 0.594ml
@13:46:39.78 delay 5 seconds
@13:46:44.81 ATV.dial :extSrc
@13:46:45.40 delay 2 seconds
@13:46:47.47 AS.seek 0.6ml
@13:46:53.85 delay 4 seconds
@13:46:57.88 ATV.dial ARV
@13:46:58.48 delay 2 seconds
@13:47:00.50 ARV.dial :carrier
@13:47:00.51 delay 2 seconds
@13:47:02.59 AS.seek 0.65ml
@13:47:53.44 delay 4 seconds
@13:47:57.47 ATV.dial PCR
@13:47:58.28 ASV.dial :waste1
@13:47:58.30 ARV.dial :air1
@13:47:58.74 Positioning Slug by 0.324ml
@13:47:58.82 AS.seek 0.326ml
@13:53:25.05 delay 5 seconds
@13:53:30.08 ATV.dial ARV
@13:53:30.89 ASV.dial :PCRlock
@13:53:31.30 delay 5 seconds
@13:53:36.33 Running ACOPP5 ProfileIPc thermocycling
@13:53:36.34 <PCR> PCR.program ProfileIPc
@13:53:36.46 ProfileIPc should complete in < 1:26:35
@13:53:36.47 PCR.run 1:53:13.75
@15:02:18.03 PCR.off
@15:02:18.57 <MFBPCR> PCR for ACOPP5 completed -- closed /var/log/esp/ACOPP521may0513h.pcr
@15:02:18.64 Cleaning PCR
@15:02:18.65 AFV.select :carrierSolution
@15:02:18.78 delay 2 seconds
@15:02:20.80 AS.reconfigure ASconfig
@15:02:20.86 AS.seek 0.5ml
@15:02:31.14 delay 1 second
@15:02:32.17 AFV.select :holdingCoil
@15:02:32.27 ASV.dial :waste1
@15:02:32.66 ATV.dial :air3
@15:02:34.02 AS.reconfigure ASslow1
@15:02:34.08 AS.seek 0.51ml
@15:02:36.34 delay 2 seconds
@15:02:38.36 ATV.dial :bleach
@15:02:39.00 AS.seek 0.81ml
@15:03:39.58 delay 3 seconds
@15:03:42.61 ATV.dial :air3
@15:03:43.21 delay 3 seconds
@15:03:46.28 AS.seek 0.835ml
@15:03:51.71 delay 3 seconds
@15:03:54.74 ATV.dial :bleach
@15:03:55.37 AS.seek 0.82ml
@15:03:58.66 delay 3 seconds
@15:04:01.69 ARV.dial :waste
@15:04:02.33 delay 3 seconds
@15:04:05.36 ATV.dial :ARV
@15:04:06.39 AS.reconfigure ASconfig
@15:04:06.45 AS.seek 0.47ml
@15:04:26.85 AFV.select :carrierSolution
@15:04:26.97 delay 2 seconds
@15:04:28.99 AS.seek 1ml
@15:04:59.74 delay 1 second
@15:05:00.77 AFV.select :holdingCoil
@15:05:00.87 delay 1 second
@15:05:01.89 AS.reconfigure ASfast
@15:05:01.95 AS.seek 0ml
@15:05:29.27 AFV.select :carrierSolution
@15:05:29.39 delay 2 seconds
@15:05:31.41 AS.reconfigure ASconfig
@15:05:31.47 AS.seek 1ml
@15:06:29.21 delay 1 second
@15:06:30.24 AFV.select :holdingCoil
@15:06:30.34 delay 1 second
@15:06:31.36 AS.reconfigure ASfast
@15:06:31.42 AS.seek 0ml
@15:06:58.75 AFV.select :carrierSolution
@15:06:58.86 delay 2 seconds
@15:07:00.88 AS.reconfigure ASconfig
@15:07:00.94 AS.seek 1ml
@15:07:59.05 delay 1 second
@15:08:00.08 AFV.select :holdingCoil
@15:08:00.18 delay 1 second
@15:08:01.20 AS.reconfigure ASfast
@15:08:01.26 AS.seek 0ml
@15:08:28.58 ARV.dial between carrier and air1
@15:08:29.12 ATV.dial :air3
@15:08:30.47 AS.reconfigure ASslow1
@15:08:30.54 AS.seek 0.01ml
@15:08:32.83 delay 2 seconds
@15:08:34.86 ATV.dial :bleach
@15:08:35.50 AS.seek 0.81ml
@15:11:16.64 delay 3 seconds
@15:11:19.67 ATV.dial :air3
@15:11:20.25 delay 3 seconds
@15:11:23.33 AS.seek 0.835ml
@15:11:28.60 delay 3 seconds
@15:11:31.63 ATV.dial :bleach
@15:11:32.27 AS.seek 0.82ml
@15:11:35.58 delay 3 seconds
@15:11:38.61 ATV.dial PCR
@15:11:39.03 AS.reconfigure ASconfig
@15:11:39.08 AS.seek 0ml
@15:12:26.46 PCR.program :bleachProfile
@15:12:26.53 PCR program should complete in < 2:45
@15:12:26.54 PCR.run 10:00
@15:15:22.34 PCR.off
@15:15:22.40 AFV.select :carrierSolution
@15:15:22.51 delay 2 seconds
@15:15:24.53 AS.seek 1ml
@15:16:22.27 delay 1 second
@15:16:23.30 AFV.select :holdingCoil
@15:16:23.40 delay 1 second
@15:16:24.42 AS.reconfigure ASfast
@15:16:24.48 AS.seek 0ml
@15:16:51.81 delay 3 seconds
@15:16:54.84 AFV.select :carrierSolution
@15:16:54.94 delay 2 seconds
@15:16:56.96 AS.reconfigure ASconfig
@15:16:57.03 AS.seek 1ml
@15:17:54.78 delay 1 second
@15:17:55.81 AFV.select :holdingCoil
@15:17:55.91 delay 1 second
@15:17:56.93 AS.reconfigure ASfast
@15:17:56.99 AS.seek 0ml
@15:18:24.33 delay 3 seconds
@15:18:27.36 AFV.select :carrierSolution
@15:18:27.46 delay 2 seconds
@15:18:29.48 AS.reconfigure ASconfig
@15:18:29.54 AS.seek 1ml
@15:19:27.35 delay 1 second
@15:19:28.38 AFV.select :holdingCoil
@15:19:28.48 delay 1 second
@15:19:29.50 AS.reconfigure ASfast
@15:19:29.56 AS.seek 0ml
@15:19:56.86 delay 3 seconds
@15:19:59.89 AFV.select :carrierSolution
@15:19:59.99 delay 2 seconds
@15:20:02.01 AS.reconfigure ASconfig
@15:20:02.08 AS.seek 1ml
@15:20:59.83 delay 1 second
@15:21:00.86 AFV.select :holdingCoil
@15:21:00.96 delay 1 second
@15:21:01.98 AS.reconfigure ASfast
@15:21:02.04 AS.seek 0ml
@15:21:29.38 delay 3 seconds
@15:21:32.41 AFV.select :carrierSolution
@15:21:32.51 delay 2 seconds
@15:21:34.53 AS.reconfigure ASconfig
@15:21:34.59 AS.seek 1ml
@15:22:32.38 delay 1 second
@15:22:33.41 AFV.select :holdingCoil
@15:22:33.51 delay 1 second
@15:22:34.53 AS.reconfigure ASnorm
@15:22:34.59 AS.seek 0ml
@15:23:25.30 <MAIN> AFV.select :carrierSolution
@15:23:25.42 delay 2 seconds
@15:23:27.44 AS.reconfigure ASconfig
@15:23:27.49 AS.seek 0.5ml
@15:23:56.53 delay 1 second
@15:23:57.55 AFV.select :holdingCoil
@15:23:57.65 ATV.dial :air1
@15:23:59.23 AS.reconfigure ASslow2
@15:23:59.28 AS.seek 0.506ml
@15:24:05.74 ATV.dial :extSrc
@15:24:07.55 AS.seek 0.512ml
@15:24:14.21 delay 5 seconds
@15:24:19.24 ATV.dial :air1
@15:24:21.05 AS.reconfigure ASslow1
@15:24:21.10 AS.seek 0.542ml
@15:24:27.52 ATV.dial :waste
@15:24:28.11 AS.reconfigure ASconfig
@15:24:28.16 AS.seek 0ml
@15:25:00.12 <MFBPCR> Setting up PCR for ACOPP5 -- writing /var/log/esp/ACOPP521may0515h.pcr
@15:25:00.14 Building PCR Slug from extSrc with MM3 mix, PP5 primer
@15:25:00.17 Priming with carrier
@15:25:00.19 ATV.dial ARV
@15:25:01.75 ARV.dial :carrier
@15:25:02.11 AS.reconfigure ASnorm
@15:25:02.18 AS.seek 0.5ml
@15:25:27.80 AFV.select :carrierSolution
@15:25:27.93 delay 2 seconds
@15:25:29.95 AS.reconfigure ASconfig
@15:25:30.02 AS.seek 1ml
@15:25:59.03 delay 1 second
@15:26:00.06 AFV.select :holdingCoil
@15:26:00.16 delay 1 second
@15:26:01.18 ATV.dial :waste
@15:26:02.95 AS.reconfigure ASnorm
@15:26:03.01 AS.seek 0ml
@15:26:53.69 AFV.select :carrierSolution
@15:26:53.81 delay 2 seconds
@15:26:55.83 AS.reconfigure ASconfig
@15:26:55.89 AS.seek 0.5ml
@15:27:24.94 delay 1 second
@15:27:25.97 AFV.select :holdingCoil
@15:27:26.07 Building Slug
@15:27:26.08 ATV.dial :ARV
@15:27:27.93 delay 2 seconds
@15:27:29.96 ARV.dial :air1
@15:27:30.37 delay 2 seconds
@15:27:32.43 AS.reconfigure ASslow2
@15:27:32.49 AS.seek 0.506ml
@15:27:38.75 delay 2 seconds
@15:27:40.78 ARV.dial :MM3
@15:27:41.90 delay 2 seconds
@15:27:43.92 Adding MM3
@15:27:44.01 AS.seek 0.524ml
@15:28:02.42 delay 25 seconds
@15:28:27.45 ARV.dial :PP5
@15:28:27.89 Adding PP5
@15:28:27.90 delay 2 seconds
@15:28:29.98 AS.seek 0.53ml
@15:28:36.42 delay 10 seconds
@15:28:46.46 ARV.dial :air3
@15:28:46.87 adding ARV air
@15:28:46.88 delay 2 seconds
@15:28:48.96 AS.seek 0.536ml
@15:28:55.28 delay 5 seconds
@15:29:00.31 ARV.dial :carrier
@15:29:01.67 delay 7 seconds
@15:29:08.75 AS.seek 0.594ml
@15:30:07.19 delay 5 seconds
@15:30:12.22 ATV.dial :extSrc
@15:30:12.83 delay 2 seconds
@15:30:14.90 AS.seek 0.6ml
@15:30:21.53 delay 4 seconds
@15:30:25.56 ATV.dial ARV
@15:30:26.14 delay 2 seconds
@15:30:28.16 ARV.dial :carrier
@15:30:28.17 delay 2 seconds
@15:30:30.25 AS.seek 0.65ml
@15:31:21.17 delay 4 seconds
@15:31:25.20 ATV.dial PCR
@15:31:26.00 ASV.dial :waste1
@15:31:26.02 ARV.dial :air1
@15:31:26.45 Positioning Slug by 0.324ml
@15:31:26.53 AS.seek 0.326ml
@15:36:52.44 delay 5 seconds
@15:36:57.47 ATV.dial ARV
@15:36:58.28 ASV.dial :PCRlock
@15:36:58.68 delay 5 seconds
@15:37:03.70 Running ACOPP5 ProfileIPc thermocycling
@15:37:03.71 <PCR> PCR.program ProfileIPc
@15:37:03.83 ProfileIPc should complete in < 1:26:35
@15:37:03.84 PCR.run 1:53:13.75
@16:45:56.40 PCR.off
@16:45:56.95 <MFBPCR> PCR for ACOPP5 completed -- closed /var/log/esp/ACOPP521may0515h.pcr
@16:45:57.03 Cleaning PCR
AFV.select :carrierSolution
@16:45:57.16 delay 2 seconds
@16:45:59.18 AS.reconfigure ASconfig
@16:45:59.24 AS.seek 0.5ml
@16:46:09.50 delay 1 second
@16:46:10.53 AFV.select :holdingCoil
@16:46:10.63 ASV.dial :waste1
@16:46:11.02 ATV.dial :air3
@16:46:12.38 AS.reconfigure ASslow1
@16:46:12.44 AS.seek 0.51ml
@16:46:14.73 delay 2 seconds
@16:46:16.75 ATV.dial :bleach
@16:46:17.41 AS.seek 0.81ml
@16:47:18.00 delay 3 seconds
@16:47:21.03 ATV.dial :air3
@16:47:21.63 delay 3 seconds
@16:47:24.71 AS.seek 0.835ml
@16:47:30.01 delay 3 seconds
@16:47:33.04 ATV.dial :bleach
@16:47:33.69 AS.seek 0.82ml
@16:47:37.03 delay 3 seconds
@16:47:40.06 ARV.dial :waste
@16:47:40.71 delay 3 seconds
@16:47:43.74 ATV.dial :ARV
@16:47:44.66 AS.reconfigure ASconfig
@16:47:44.71 AS.seek 0.47ml
@16:48:05.13 AFV.select :carrierSolution
@16:48:05.25 delay 2 seconds
@16:48:07.27 AS.seek 1ml
@16:48:38.07 delay 1 second
@16:48:39.10 AFV.select :holdingCoil
@16:48:39.23 delay 1 second
@16:48:40.26 AS.reconfigure ASfast
@16:48:40.32 AS.seek 0ml
@16:49:07.63 AFV.select :carrierSolution
@16:49:07.75 delay 2 seconds
@16:49:09.77 AS.reconfigure ASconfig
@16:49:09.83 AS.seek 1ml
@16:50:07.57 delay 1 second
@16:50:08.60 AFV.select :holdingCoil
@16:50:08.70 delay 1 second
@16:50:09.72 AS.reconfigure ASfast
@16:50:09.78 AS.seek 0ml
@16:50:37.08 AFV.select :carrierSolution
@16:50:37.20 delay 2 seconds
@16:50:39.22 AS.reconfigure ASconfig
@16:50:39.28 AS.seek 1ml
@16:51:37.03 delay 1 second
@16:51:38.06 AFV.select :holdingCoil
@16:51:38.16 delay 1 second
@16:51:39.18 AS.reconfigure ASfast
@16:51:39.24 AS.seek 0ml
@16:52:06.58 ARV.dial between carrier and air1
@16:52:07.12 ATV.dial :air3
@16:52:08.47 AS.reconfigure ASslow1
@16:52:08.53 AS.seek 0.01ml
@16:52:10.82 delay 2 seconds
@16:52:12.85 ATV.dial :bleach
@16:52:13.48 AS.seek 0.81ml
@16:54:54.61 delay 3 seconds
@16:54:57.64 ATV.dial :air3
@16:54:58.22 delay 3 seconds
@16:55:01.29 AS.seek 0.835ml
@16:55:06.58 delay 3 seconds
@16:55:09.61 ATV.dial :bleach
@16:55:10.25 AS.seek 0.82ml
@16:55:13.53 delay 3 seconds
@16:55:16.56 ATV.dial PCR
@16:55:16.98 AS.reconfigure ASconfig
@16:55:17.03 AS.seek 0ml
@16:56:04.44 PCR.program :bleachProfile
@16:56:04.51 PCR program should complete in < 2:45
@16:56:04.52 PCR.run 10:00
@16:59:00.69 PCR.off
@16:59:00.74 AFV.select :carrierSolution
@16:59:00.85 delay 2 seconds
@16:59:02.87 AS.seek 1ml
@17:00:00.60 delay 1 second
@17:00:01.63 AFV.select :holdingCoil
@17:00:01.73 delay 1 second
@17:00:02.75 AS.reconfigure ASfast
@17:00:02.81 AS.seek 0ml
@17:00:30.16 delay 3 seconds
@17:00:33.19 AFV.select :carrierSolution
@17:00:33.29 delay 2 seconds
@17:00:35.31 AS.reconfigure ASconfig
@17:00:35.38 AS.seek 1ml
@17:01:33.11 delay 1 second
@17:01:34.14 AFV.select :holdingCoil
@17:01:34.24 delay 1 second
@17:01:35.26 AS.reconfigure ASfast
@17:01:35.32 AS.seek 0ml
@17:02:02.64 delay 3 seconds
@17:02:05.67 AFV.select :carrierSolution
@17:02:05.77 delay 2 seconds
@17:02:07.79 AS.reconfigure ASconfig
@17:02:07.86 AS.seek 1ml
@17:03:05.61 delay 1 second
@17:03:06.64 AFV.select :holdingCoil
@17:03:06.74 delay 1 second
@17:03:07.76 AS.reconfigure ASfast
@17:03:07.82 AS.seek 0ml
@17:03:35.16 delay 3 seconds
@17:03:38.19 AFV.select :carrierSolution
@17:03:38.29 delay 2 seconds
@17:03:40.31 AS.reconfigure ASconfig
@17:03:40.38 AS.seek 1ml
@17:04:38.13 delay 1 second
@17:04:39.16 AFV.select :holdingCoil
@17:04:39.26 delay 1 second
@17:04:40.28 AS.reconfigure ASfast
@17:04:40.34 AS.seek 0ml
@17:05:07.67 delay 3 seconds
@17:05:10.70 AFV.select :carrierSolution
@17:05:10.80 delay 2 seconds
@17:05:12.82 AS.reconfigure ASconfig
@17:05:12.88 AS.seek 1ml
@17:06:10.64 delay 1 second
@17:06:11.67 AFV.select :holdingCoil
@17:06:11.77 delay 1 second
@17:06:12.79 AS.reconfigure ASnorm
@17:06:12.85 AS.seek 0ml
@17:07:03.57 <MAIN> AFV.select :carrierSolution
@17:07:03.68 delay 2 seconds
@17:07:05.70 AS.reconfigure ASconfig
@17:07:05.76 AS.seek 0.5ml
@17:07:34.82 delay 1 second
@17:07:35.85 AFV.select :holdingCoil
@17:07:35.95 ATV.dial :air1
@17:07:37.52 AS.reconfigure ASslow2
@17:07:37.57 AS.seek 0.51ml
@17:07:48.08 delay 5 seconds
@17:07:53.11 ATV.dial :extSrc
@17:07:54.88 AS.seek 0.53ml
@17:08:15.21 delay 10 seconds
@17:08:25.24 ATV.dial :air1
@17:08:27.03 AS.reconfigure ASslow1
@17:08:27.08 AS.seek 0.56ml
@17:08:33.44 ATV.dial :waste
@17:08:34.04 AS.reconfigure ASconfig
@17:08:34.09 AS.seek 0ml
@17:09:06.73 <MFBPCR> Setting up PCR for ACOPP5 -- writing /var/log/esp/ACOPP521may0517h.pcr
@17:09:06.75 Building PCR Slug from extSrc with MM3 mix, PP5 primer
@17:09:06.77 Priming with carrier
@17:09:06.79 ATV.dial ARV
@17:09:08.35 ARV.dial :carrier
@17:09:08.70 AS.reconfigure ASnorm
@17:09:08.77 AS.seek 0.5ml
@17:09:34.30 AFV.select :carrierSolution
@17:09:34.43 delay 2 seconds
@17:09:36.45 AS.reconfigure ASconfig
@17:09:36.51 AS.seek 1ml
@17:10:05.53 delay 1 second
@17:10:06.56 AFV.select :holdingCoil
@17:10:06.66 delay 1 second
@17:10:08.03 ATV.dial :waste
@17:10:09.81 AS.reconfigure ASnorm
@17:10:09.87 AS.seek 0ml
@17:11:00.55 AFV.select :carrierSolution
@17:11:00.68 delay 2 seconds
@17:11:02.70 AS.reconfigure ASconfig
@17:11:02.76 AS.seek 0.5ml
@17:11:31.82 delay 1 second
@17:11:32.85 AFV.select :holdingCoil
@17:11:32.95 Building Slug
@17:11:32.96 ATV.dial :ARV
@17:11:34.72 delay 2 seconds
@17:11:36.74 ARV.dial :air1
@17:11:37.16 delay 2 seconds
@17:11:39.22 AS.reconfigure ASslow2
@17:11:39.28 AS.seek 0.506ml
@17:11:45.72 delay 2 seconds
@17:11:47.75 ARV.dial :MM3
@17:11:48.87 delay 2 seconds
@17:11:50.89 Adding MM3
@17:11:50.98 AS.seek 0.524ml
@17:12:09.28 delay 25 seconds
@17:12:34.31 ARV.dial :PP5
@17:12:34.73 Adding PP5
@17:12:34.74 delay 2 seconds
@17:12:36.82 AS.seek 0.53ml
@17:12:43.22 delay 10 seconds
@17:12:53.26 ARV.dial :air3
@17:12:53.68 adding ARV air
@17:12:53.69 delay 2 seconds
@17:12:55.77 AS.seek 0.536ml
@17:13:02.17 delay 5 seconds
@17:13:07.20 ARV.dial :carrier
@17:13:08.54 delay 7 seconds
@17:13:15.62 AS.seek 0.594ml
@17:14:14.36 delay 5 seconds
@17:14:19.39 ATV.dial :extSrc
@17:14:19.98 delay 2 seconds
@17:14:22.05 AS.seek 0.6ml
@17:14:28.38 delay 4 seconds
@17:14:32.41 ATV.dial ARV
@17:14:32.98 delay 2 seconds
@17:14:35.00 ARV.dial :carrier
@17:14:35.01 delay 2 seconds
@17:14:37.09 AS.seek 0.65ml
@17:15:27.74 delay 4 seconds
@17:15:31.77 ATV.dial PCR
@17:15:32.57 ASV.dial :waste1
@17:15:32.59 ARV.dial :air1
@17:15:33.02 Positioning Slug by 0.324ml
@17:15:33.11 AS.seek 0.326ml
@17:20:59.21 delay 5 seconds
@17:21:04.24 ATV.dial ARV
@17:21:05.05 ASV.dial :PCRlock
@17:21:05.47 delay 5 seconds
@17:21:10.49 Running ACOPP5 ProfileIPc thermocycling
@17:21:10.50 <PCR> PCR.program ProfileIPc
@17:21:10.62 ProfileIPc should complete in < 1:26:35
@17:21:10.63 PCR.run 1:53:13.75
@18:30:03.69 PCR.off
@18:30:04.24 <MFBPCR> PCR for ACOPP5 completed -- closed /var/log/esp/ACOPP521may0517h.pcr
@18:30:04.32 Cleaning PCR
@18:30:04.33 AFV.select :carrierSolution
@18:30:04.45 delay 2 seconds
@18:30:06.47 AS.reconfigure ASconfig
@18:30:06.53 AS.seek 0.5ml
@18:30:16.80 delay 1 second
@18:30:17.83 AFV.select :holdingCoil
@18:30:17.93 ASV.dial :waste1
@18:30:18.33 ATV.dial :air3
@18:30:19.69 AS.reconfigure ASslow1
@18:30:19.75 AS.seek 0.51ml
@18:30:22.03 delay 2 seconds
@18:30:24.06 ATV.dial :bleach
@18:30:24.70 AS.seek 0.81ml
@18:31:25.36 delay 3 seconds
@18:31:28.39 ATV.dial :air3
@18:31:28.97 delay 3 seconds
@18:31:32.04 AS.seek 0.835ml
@18:31:37.36 delay 3 seconds
@18:31:40.39 ATV.dial :bleach
@18:31:41.03 AS.seek 0.82ml
@18:31:44.35 delay 3 seconds
@18:31:47.38 ARV.dial :waste
@18:31:48.00 delay 3 seconds
@18:31:51.03 ATV.dial :ARV
@18:31:51.95 AS.reconfigure ASconfig
@18:31:52.01 AS.seek 0.47ml
@18:32:12.41 AFV.select :carrierSolution
@18:32:12.53 delay 2 seconds
@18:32:14.55 AS.seek 1ml
@18:32:45.33 delay 1 second
@18:32:46.36 AFV.select :holdingCoil
@18:32:46.46 delay 1 second
@18:32:47.48 AS.reconfigure ASfast
@18:32:47.54 AS.seek 0ml
@18:33:14.85 AFV.select :carrierSolution
@18:33:14.97 delay 2 seconds
@18:33:16.99 AS.reconfigure ASconfig
@18:33:17.05 AS.seek 1ml
@18:34:14.80 delay 1 second
@18:34:15.83 AFV.select :holdingCoil
@18:34:15.93 delay 1 second
@18:34:16.95 AS.reconfigure ASfast
@18:34:17.01 AS.seek 0ml
@18:34:44.35 AFV.select :carrierSolution
@18:34:44.47 delay 2 seconds
@18:34:46.49 AS.reconfigure ASconfig
@18:34:46.55 AS.seek 1ml
@18:35:44.30 delay 1 second
@18:35:45.33 AFV.select :holdingCoil
@18:35:45.43 delay 1 second
@18:35:46.45 AS.reconfigure ASfast
@18:35:46.51 AS.seek 0ml
@18:36:13.86 ARV.dial between carrier and air1
@18:36:14.76 ATV.dial :air3
@18:36:16.11 AS.reconfigure ASslow1
@18:36:16.17 AS.seek 0.01ml
@18:36:18.49 delay 2 seconds
@18:36:20.52 ATV.dial :bleach
@18:36:21.17 AS.seek 0.81ml
@18:39:02.30 delay 3 seconds
@18:39:05.33 ATV.dial :air3
@18:39:05.93 delay 3 seconds
@18:39:09.00 AS.seek 0.835ml
@18:39:14.39 delay 3 seconds
@18:39:17.42 ATV.dial :bleach
@18:39:18.06 AS.seek 0.82ml
@18:39:21.35 delay 3 seconds
@18:39:24.38 ATV.dial PCR
@18:39:24.79 AS.reconfigure ASconfig
@18:39:24.85 AS.seek 0ml
@18:40:12.24 PCR.program :bleachProfile
@18:40:12.31 PCR program should complete in < 2:45
@18:40:12.32 PCR.run 10:00
@18:43:08.10 PCR.off
@18:43:08.14 AFV.select :carrierSolution
@18:43:08.26 delay 2 seconds
@18:43:10.28 AS.seek 1ml
@18:44:08.03 delay 1 second
@18:44:09.06 AFV.select :holdingCoil
@18:44:09.16 delay 1 second
@18:44:10.18 AS.reconfigure ASfast
@18:44:10.24 AS.seek 0ml
@18:44:37.58 delay 3 seconds
@18:44:40.61 AFV.select :carrierSolution
@18:44:40.71 delay 2 seconds
@18:44:42.73 AS.reconfigure ASconfig
@18:44:42.80 AS.seek 1ml
@18:45:40.55 delay 1 second
@18:45:41.58 AFV.select :holdingCoil
@18:45:41.68 delay 1 second
@18:45:42.70 AS.reconfigure ASfast
@18:45:42.76 AS.seek 0ml
@18:46:10.10 delay 3 seconds
@18:46:13.13 AFV.select :carrierSolution
@18:46:13.23 delay 2 seconds
@18:46:15.25 AS.reconfigure ASconfig
@18:46:15.33 AS.seek 1ml
@18:47:13.07 delay 1 second
@18:47:14.10 AFV.select :holdingCoil
@18:47:14.20 delay 1 second
@18:47:15.22 AS.reconfigure ASfast
@18:47:15.28 AS.seek 0ml
@18:47:42.61 delay 3 seconds
@18:47:45.64 AFV.select :carrierSolution
@18:47:45.74 delay 2 seconds
@18:47:47.76 AS.reconfigure ASconfig
@18:47:47.83 AS.seek 1ml
@18:48:45.59 delay 1 second
@18:48:46.62 AFV.select :holdingCoil
@18:48:46.72 delay 1 second
@18:48:47.74 AS.reconfigure ASfast
@18:48:47.80 AS.seek 0ml
@18:49:15.13 delay 3 seconds
@18:49:18.16 AFV.select :carrierSolution
@18:49:18.26 delay 2 seconds
@18:49:20.28 AS.reconfigure ASconfig
@18:49:20.35 AS.seek 1ml
@18:50:18.13 delay 1 second
@18:50:19.16 AFV.select :holdingCoil
@18:50:19.26 delay 1 second
@18:50:20.61 AS.reconfigure ASnorm
@18:50:20.72 AS.seek 0ml
@18:51:11.44 <MAIN> AFV.select :carrierSolution
@18:51:11.54 delay 2 seconds
@18:51:13.56 AS.reconfigure ASconfig
@18:51:13.61 AS.seek 0.5ml
@18:51:42.64 delay 1 second
@18:51:43.67 AFV.select :holdingCoil
@18:51:43.77 ATV.dial :air1
@18:51:45.33 AS.reconfigure ASslow2
@18:51:45.38 AS.seek 0.506ml
@18:51:51.71 ATV.dial :extSrc
@18:51:53.49 AS.seek 0.512ml
@18:52:00.27 delay 5 seconds
@18:52:05.30 ATV.dial :air1
@18:52:07.11 AS.reconfigure ASslow1
@18:52:07.16 AS.seek 0.542ml
@18:52:13.47 ATV.dial :waste
@18:52:14.04 AS.reconfigure ASconfig
@18:52:14.10 AS.seek 0ml
@18:52:45.71 <MFBPCR> Setting up PCR for ACOPP5 -- writing /var/log/esp/ACOPP521may0518h.pcr
@18:52:45.73 Building PCR Slug from extSrc with MM3 mix, PP5 primer
@18:52:45.76 Priming with carrier
@18:52:45.78 ATV.dial ARV
@18:52:47.33 ARV.dial :carrier
@18:52:47.69 AS.reconfigure ASnorm
@18:52:47.76 AS.seek 0.5ml
@18:53:13.35 AFV.select :carrierSolution
@18:53:13.48 delay 2 seconds
@18:53:15.50 AS.reconfigure ASconfig
@18:53:15.57 AS.seek 1ml
@18:53:44.60 delay 1 second
@18:53:45.63 AFV.select :holdingCoil
@18:53:45.73 delay 1 second
@18:53:46.77 ATV.dial :waste
@18:53:48.53 AS.reconfigure ASnorm
@18:53:48.59 AS.seek 0ml
@18:54:39.32 AFV.select :carrierSolution
@18:54:39.44 delay 2 seconds
@18:54:41.46 AS.reconfigure ASconfig
@18:54:41.52 AS.seek 0.5ml
@18:55:10.57 delay 1 second
@18:55:11.60 AFV.select :holdingCoil
@18:55:11.70 Building Slug
@18:55:11.71 ATV.dial :ARV
@18:55:13.47 delay 2 seconds
@18:55:15.49 ARV.dial :air1
@18:55:15.91 delay 2 seconds
@18:55:17.97 AS.reconfigure ASslow2
@18:55:18.03 AS.seek 0.506ml
@18:55:24.36 delay 2 seconds
@18:55:26.39 ARV.dial :MM3
@18:55:27.50 delay 2 seconds
@18:55:29.52 Adding MM3
@18:55:29.59 AS.seek 0.524ml
@18:55:48.16 delay 25 seconds
@18:56:13.19 ARV.dial :PP5
@18:56:13.61 Adding PP5
@18:56:13.62 delay 2 seconds
@18:56:15.70 AS.seek 0.53ml
@18:56:21.88 delay 10 seconds
@18:56:31.92 ARV.dial :air3
@18:56:32.34 adding ARV air
@18:56:32.35 delay 2 seconds
@18:56:34.43 AS.seek 0.536ml
@18:56:40.83 delay 5 seconds
@18:56:45.86 ARV.dial :carrier
@18:56:47.22 delay 7 seconds
@18:56:54.30 AS.seek 0.594ml
@18:57:52.91 delay 5 seconds
@18:57:57.94 ATV.dial :extSrc
@18:57:58.53 delay 2 seconds
@18:58:00.61 AS.seek 0.6ml
@18:58:06.91 delay 4 seconds
@18:58:10.94 ATV.dial ARV
@18:58:11.50 delay 2 seconds
@18:58:13.53 ARV.dial :carrier
@18:58:13.54 delay 2 seconds
@18:58:15.62 AS.seek 0.65ml
@18:59:06.46 delay 4 seconds
@18:59:10.49 ATV.dial PCR
@18:59:11.30 ASV.dial :waste1
@18:59:11.32 ARV.dial :air1
@18:59:11.76 Positioning Slug by 0.324ml
@18:59:11.85 AS.seek 0.326ml
@19:04:38.09 delay 5 seconds
@19:04:43.11 ATV.dial ARV
@19:04:43.93 ASV.dial :PCRlock
@19:04:44.34 delay 5 seconds
@19:04:49.36 Running ACOPP5 ProfileIPc thermocycling
@19:04:49.38 <PCR> PCR.program ProfileIPc
@19:04:49.50 ProfileIPc should complete in < 1:26:35
@19:04:49.51 PCR.run 1:53:13.75
@20:13:42.94 PCR.off
@20:13:43.50 <MFBPCR> PCR for ACOPP5 completed -- closed /var/log/esp/ACOPP521may0518h.pcr
@20:13:43.57 Cleaning PCR
@20:13:43.58 AFV.select :carrierSolution
@20:13:43.70 delay 2 seconds
@20:13:45.72 AS.reconfigure ASconfig
@20:13:45.78 AS.seek 0.5ml
@20:13:56.11 delay 1 second
@20:13:57.14 AFV.select :holdingCoil
@20:13:57.24 ASV.dial :waste1
@20:13:57.63 ATV.dial :air3
@20:13:58.99 AS.reconfigure ASslow1
@20:13:59.05 AS.seek 0.51ml
@20:14:01.32 delay 2 seconds
@20:14:03.35 ATV.dial :bleach
@20:14:04.00 AS.seek 0.81ml
@20:15:04.57 delay 3 seconds
@20:15:07.60 ATV.dial :air3
@20:15:08.55 delay 3 seconds
@20:15:11.63 AS.seek 0.835ml
@20:15:17.03 delay 3 seconds
@20:15:20.06 ATV.dial :bleach
@20:15:20.70 AS.seek 0.82ml
@20:15:23.97 delay 3 seconds
@20:15:27.00 ARV.dial :waste
@20:15:27.64 delay 3 seconds
@20:15:30.67 ATV.dial :ARV
@20:15:31.59 AS.reconfigure ASconfig
@20:15:31.65 AS.seek 0.47ml
@20:15:52.07 AFV.select :carrierSolution
@20:15:52.19 delay 2 seconds
@20:15:54.21 AS.seek 1ml
@20:16:24.97 delay 1 second
@20:16:26.00 AFV.select :holdingCoil
@20:16:26.10 delay 1 second
@20:16:27.12 AS.reconfigure ASfast
@20:16:27.18 AS.seek 0ml
@20:16:54.52 AFV.select :carrierSolution
@20:16:54.64 delay 2 seconds
@20:16:56.66 AS.reconfigure ASconfig
@20:16:56.72 AS.seek 1ml
@20:17:54.47 delay 1 second
@20:17:55.50 AFV.select :holdingCoil
@20:17:55.60 delay 1 second
@20:17:56.62 AS.reconfigure ASfast
@20:17:56.68 AS.seek 0ml
@20:18:24.02 AFV.select :carrierSolution
@20:18:24.14 delay 2 seconds
@20:18:26.16 AS.reconfigure ASconfig
@20:18:26.22 AS.seek 1ml
@20:19:24.02 delay 1 second
@20:19:25.05 AFV.select :holdingCoil
@20:19:25.15 delay 1 second
@20:19:26.17 AS.reconfigure ASfast
@20:19:26.23 AS.seek 0ml
@20:19:53.57 ARV.dial between carrier and air1
@20:19:54.11 ATV.dial :air3
@20:19:55.45 AS.reconfigure ASslow1
@20:19:55.51 AS.seek 0.01ml
@20:19:57.86 delay 2 seconds
@20:19:59.89 ATV.dial :bleach
@20:20:00.55 AS.seek 0.81ml
@20:22:41.72 delay 3 seconds
@20:22:44.75 ATV.dial :air3
@20:22:45.33 delay 3 seconds
@20:22:48.41 AS.seek 0.835ml
@20:22:53.78 delay 3 seconds
@20:22:56.81 ATV.dial :bleach
@20:22:57.45 AS.seek 0.82ml
@20:23:00.88 delay 3 seconds
@20:23:03.91 ATV.dial PCR
@20:23:04.33 AS.reconfigure ASconfig
@20:23:04.38 AS.seek 0ml
@20:23:51.82 PCR.program :bleachProfile
@20:23:51.90 PCR program should complete in < 2:45
@20:23:51.91 PCR.run 10:00
@20:26:47.69 PCR.off
@20:26:48.10 AFV.select :carrierSolution
@20:26:48.23 delay 2 seconds
@20:26:50.25 AS.seek 1ml
@20:27:48.00 delay 1 second
@20:27:49.03 AFV.select :holdingCoil
@20:27:49.13 delay 1 second
@20:27:50.15 AS.reconfigure ASfast
@20:27:50.21 AS.seek 0ml
@20:28:17.57 delay 3 seconds
@20:28:20.60 AFV.select :carrierSolution
@20:28:20.70 delay 2 seconds
@20:28:22.72 AS.reconfigure ASconfig
@20:28:22.79 AS.seek 1ml
@20:29:20.53 delay 1 second
@20:29:21.56 AFV.select :holdingCoil
@20:29:21.66 delay 1 second
@20:29:22.68 AS.reconfigure ASfast
@20:29:22.74 AS.seek 0ml
@20:29:50.10 delay 3 seconds
@20:29:53.13 AFV.select :carrierSolution
@20:29:53.24 delay 2 seconds
@20:29:55.26 AS.reconfigure ASconfig
@20:29:55.33 AS.seek 1ml
@20:30:53.08 delay 1 second
@20:30:54.11 AFV.select :holdingCoil
@20:30:54.21 delay 1 second
@20:30:55.23 AS.reconfigure ASfast
@20:30:55.29 AS.seek 0ml
@20:31:22.66 delay 3 seconds
@20:31:25.69 AFV.select :carrierSolution
@20:31:25.79 delay 2 seconds
@20:31:27.81 AS.reconfigure ASconfig
@20:31:27.88 AS.seek 1ml
@20:32:25.66 delay 1 second
@20:32:26.69 AFV.select :holdingCoil
@20:32:26.79 delay 1 second
@20:32:27.81 AS.reconfigure ASfast
@20:32:27.87 AS.seek 0ml
@20:32:55.21 delay 3 seconds
@20:32:58.24 AFV.select :carrierSolution
@20:32:58.34 delay 2 seconds
@20:33:00.36 AS.reconfigure ASconfig
@20:33:00.42 AS.seek 1ml
@20:33:58.16 delay 1 second
@20:33:59.19 AFV.select :holdingCoil
@20:33:59.29 delay 1 second
@20:34:00.31 AS.reconfigure ASnorm
@20:34:00.37 AS.seek 0ml
@20:34:51.08 <MAIN> AFV.select :carrierSolution
@20:34:51.18 delay 2 seconds
@20:34:53.20 AS.reconfigure ASconfig
@20:34:53.25 AS.seek 0.5ml
@20:35:22.27 delay 1 second
@20:35:23.30 AFV.select :holdingCoil
@20:35:23.40 ATV.dial :air1
@20:35:24.95 AS.reconfigure ASslow1
@20:35:25.00 AS.seek 0.52ml
@20:35:29.28 delay 5 seconds
@20:35:34.31 ATV.dial :extSrc
@20:35:36.08 AS.reconfigure ASslow2
@20:35:36.13 AS.seek 0.545ml
@20:36:01.77 delay 10 seconds
@20:36:11.80 ATV.dial :air1
@20:36:13.58 AS.reconfigure ASslow1
@20:36:13.63 AS.seek 0.575ml
@20:36:20.00 ATV.dial :waste
@20:36:20.58 AS.reconfigure ASconfig
@20:36:20.64 AS.seek 0ml
@20:36:54.11 <MFBPCR> Setting up PCR for ACOPP5 -- writing /var/log/esp/ACOPP521may0520h.pcr
@20:36:54.13 Building PCR Slug from extSrc with MM3 mix, PP5 primer
@20:36:54.16 Priming with carrier
@20:36:54.18 ATV.dial ARV
@20:36:55.74 ARV.dial :carrier
@20:36:56.09 AS.reconfigure ASnorm
@20:36:56.16 AS.seek 0.5ml
@20:37:21.71 AFV.select :carrierSolution
@20:37:21.84 delay 2 seconds
@20:37:23.86 AS.reconfigure ASconfig
@20:37:23.92 AS.seek 1ml
@20:37:52.94 delay 1 second
@20:37:53.97 AFV.select :holdingCoil
@20:37:54.07 delay 1 second
@20:37:55.09 ATV.dial :waste
@20:37:56.86 AS.reconfigure ASnorm
@20:37:56.92 AS.seek 0ml
@20:38:47.58 AFV.select :carrierSolution
@20:38:47.71 delay 2 seconds
@20:38:49.73 AS.reconfigure ASconfig
@20:38:49.79 AS.seek 0.5ml
@20:39:18.80 delay 1 second
@20:39:19.83 AFV.select :holdingCoil
@20:39:19.93 Building Slug
@20:39:19.94 ATV.dial :ARV
@20:39:21.69 delay 2 seconds
@20:39:23.71 ARV.dial :air1
@20:39:24.12 delay 2 seconds
@20:39:26.18 AS.reconfigure ASslow2
@20:39:26.25 AS.seek 0.506ml
@20:39:32.43 delay 2 seconds
@20:39:34.46 ARV.dial :MM3
@20:39:35.58 delay 2 seconds
@20:39:37.60 Adding MM3
@20:39:37.68 AS.seek 0.524ml
@20:39:55.93 delay 25 seconds
@20:40:20.96 ARV.dial :PP5
@20:40:21.39 Adding PP5
@20:40:21.40 delay 2 seconds
@20:40:23.48 AS.seek 0.53ml
@20:40:29.80 delay 10 seconds
@20:40:39.84 ARV.dial :air3
@20:40:40.25 adding ARV air
@20:40:40.26 delay 2 seconds
@20:40:42.34 AS.seek 0.536ml
@20:40:48.52 delay 5 seconds
@20:40:53.55 ARV.dial :carrier
@20:40:54.88 delay 7 seconds
@20:41:01.96 AS.seek 0.594ml
@20:42:00.43 delay 5 seconds
@20:42:05.46 ATV.dial :extSrc
@20:42:06.04 delay 2 seconds
@20:42:08.11 AS.seek 0.6ml
@20:42:14.46 delay 4 seconds
@20:42:18.49 ATV.dial ARV
@20:42:19.08 delay 2 seconds
@20:42:21.10 ARV.dial :carrier
@20:42:21.11 delay 2 seconds
@20:42:23.58 AS.seek 0.65ml
@20:43:14.30 delay 4 seconds
@20:43:18.33 ATV.dial PCR
@20:43:19.13 ASV.dial :waste1
@20:43:19.15 ARV.dial :air1
@20:43:19.59 Positioning Slug by 0.324ml
@20:43:19.68 AS.seek 0.326ml
@20:48:45.80 delay 5 seconds
@20:48:50.83 ATV.dial ARV
@20:48:51.64 ASV.dial :PCRlock
@20:48:52.06 delay 5 seconds
@20:48:57.08 Running ACOPP5 ProfileIPc thermocycling
@20:48:57.09 <PCR> PCR.program ProfileIPc
@20:48:57.21 ProfileIPc should complete in < 1:26:35
@20:48:57.22 PCR.run 1:53:13.75
@21:57:41.36 PCR.off
@21:57:41.91 <MFBPCR> PCR for ACOPP5 completed -- closed /var/log/esp/ACOPP521may0520h.pcr
@21:57:41.99 Cleaning PCR
@21:57:42.00 AFV.select :carrierSolution
@21:57:42.12 delay 2 seconds
@21:57:44.14 AS.reconfigure ASconfig
@21:57:44.20 AS.seek 0.5ml
@21:57:54.54 delay 1 second
@21:57:55.57 AFV.select :holdingCoil
@21:57:55.67 ASV.dial :waste1
@21:57:56.07 ATV.dial :air3
@21:57:57.41 AS.reconfigure ASslow1
@21:57:57.47 AS.seek 0.51ml
@21:57:59.75 delay 2 seconds
@21:58:01.78 ATV.dial :bleach
@21:58:02.42 AS.seek 0.81ml
@21:59:03.02 delay 3 seconds
@21:59:06.05 ATV.dial :air3
@21:59:06.63 delay 3 seconds
@21:59:09.71 AS.seek 0.835ml
@21:59:15.01 delay 3 seconds
@21:59:18.04 ATV.dial :bleach
@21:59:18.67 AS.seek 0.82ml
@21:59:21.97 delay 3 seconds
@21:59:25.00 ARV.dial :waste
@21:59:25.63 delay 3 seconds
@21:59:28.66 ATV.dial :ARV
@21:59:29.60 AS.reconfigure ASconfig
@21:59:29.66 AS.seek 0.47ml
@21:59:50.07 AFV.select :carrierSolution
@21:59:50.19 delay 2 seconds
@21:59:52.21 AS.seek 1ml
@22:00:22.99 delay 1 second
@22:00:24.02 AFV.select :holdingCoil
@22:00:24.12 delay 1 second
@22:00:25.14 AS.reconfigure ASfast
@22:00:25.20 AS.seek 0ml
@22:00:52.54 AFV.select :carrierSolution
@22:00:52.66 delay 2 seconds
@22:00:54.68 AS.reconfigure ASconfig
@22:00:54.74 AS.seek 1ml
@22:01:52.47 delay 1 second
@22:01:53.50 AFV.select :holdingCoil
@22:01:53.60 delay 1 second
@22:01:54.62 AS.reconfigure ASfast
@22:01:54.68 AS.seek 0ml
@22:02:22.00 AFV.select :carrierSolution
@22:02:22.12 delay 2 seconds
@22:02:24.14 AS.reconfigure ASconfig
@22:02:24.20 AS.seek 1ml
@22:03:21.94 delay 1 second
@22:03:22.97 AFV.select :holdingCoil
@22:03:23.07 delay 1 second
@22:03:24.09 AS.reconfigure ASfast
@22:03:24.15 AS.seek 0ml
@22:03:51.44 ARV.dial between carrier and air1
@22:03:51.97 ATV.dial :air3
@22:03:53.31 AS.reconfigure ASslow1
@22:03:53.37 AS.seek 0.01ml
@22:03:55.64 delay 2 seconds
@22:03:57.67 ATV.dial :bleach
@22:03:58.31 AS.seek 0.81ml
@22:06:39.43 delay 3 seconds
@22:06:42.46 ATV.dial :air3
@22:06:43.04 delay 3 seconds
@22:06:46.12 AS.seek 0.835ml
@22:06:51.39 delay 3 seconds
@22:06:54.42 ATV.dial :bleach
@22:06:55.06 AS.seek 0.82ml
@22:06:58.38 delay 3 seconds
@22:07:01.41 ATV.dial PCR
@22:07:01.83 AS.reconfigure ASconfig
@22:07:01.88 AS.seek 0ml
@22:07:49.30 PCR.program :bleachProfile
@22:07:49.37 PCR program should complete in < 2:45
@22:07:49.38 PCR.run 10:00
@22:10:45.55 PCR.off
@22:10:45.60 AFV.select :carrierSolution
@22:10:45.71 delay 2 seconds
@22:10:47.73 AS.seek 1ml
@22:11:45.54 delay 1 second
@22:11:46.57 AFV.select :holdingCoil
@22:11:46.67 delay 1 second
@22:11:47.69 AS.reconfigure ASfast
@22:11:47.75 AS.seek 0ml
@22:12:15.10 delay 3 seconds
@22:12:18.13 AFV.select :carrierSolution
@22:12:18.23 delay 2 seconds
@22:12:20.25 AS.reconfigure ASconfig
@22:12:20.32 AS.seek 1ml
@22:13:18.10 delay 1 second
@22:13:19.13 AFV.select :holdingCoil
@22:13:19.23 delay 1 second
@22:13:20.25 AS.reconfigure ASfast
@22:13:20.31 AS.seek 0ml
@22:13:47.64 delay 3 seconds
@22:13:50.67 AFV.select :carrierSolution
@22:13:50.77 delay 2 seconds
@22:13:52.79 AS.reconfigure ASconfig
@22:13:52.86 AS.seek 1ml
@22:14:50.60 delay 1 second
@22:14:51.63 AFV.select :holdingCoil
@22:14:51.73 delay 1 second
@22:14:52.75 AS.reconfigure ASfast
@22:14:52.81 AS.seek 0ml
@22:15:20.15 delay 3 seconds
@22:15:23.18 AFV.select :carrierSolution
@22:15:23.28 delay 2 seconds
@22:15:25.30 AS.reconfigure ASconfig
@22:15:25.37 AS.seek 1ml
@22:16:23.14 delay 1 second
@22:16:24.17 AFV.select :holdingCoil
@22:16:24.27 delay 1 second
@22:16:25.29 AS.reconfigure ASfast
@22:16:25.35 AS.seek 0ml
@22:16:52.66 delay 3 seconds
@22:16:55.69 AFV.select :carrierSolution
@22:16:55.79 delay 2 seconds
@22:16:57.81 AS.reconfigure ASconfig
@22:16:57.88 AS.seek 1ml
@22:17:55.61 delay 1 second
@22:17:56.64 AFV.select :holdingCoil
@22:17:56.74 delay 1 second
@22:17:57.76 AS.reconfigure ASnorm
@22:17:57.82 AS.seek 0ml
@22:18:48.51 <MAIN> AFV.select :carrierSolution
@22:18:48.63 delay 2 seconds
@22:18:50.65 AS.reconfigure ASconfig
@22:18:50.70 AS.seek 0.5ml
@22:19:19.72 delay 1 second
@22:19:20.75 AFV.select :holdingCoil
@22:19:20.85 ATV.dial :air1
@22:19:22.41 AS.reconfigure ASslow2
@22:19:22.46 AS.seek 0.506ml
@22:19:29.18 ATV.dial :extSrc
@22:19:30.96 AS.seek 0.512ml
@22:19:37.25 delay 5 seconds
@22:19:42.28 ATV.dial :air1
@22:19:44.10 AS.reconfigure ASslow1
@22:19:44.15 AS.seek 0.542ml
@22:19:50.44 ATV.dial :waste
@22:19:51.03 AS.reconfigure ASconfig
@22:19:51.08 AS.seek 0ml
@22:20:22.69 <MFBPCR> Setting up PCR for ACOPP5 -- writing /var/log/esp/ACOPP521may0522h.pcr
@22:20:22.71 Building PCR Slug from extSrc with MM3 mix, PP5 primer
@22:20:22.74 Priming with carrier
@22:20:22.75 ATV.dial ARV
@22:20:24.32 ARV.dial :carrier
@22:20:24.67 AS.reconfigure ASnorm
@22:20:24.73 AS.seek 0.5ml
@22:20:50.27 AFV.select :carrierSolution
@22:20:50.40 delay 2 seconds
@22:20:52.42 AS.reconfigure ASconfig
@22:20:52.49 AS.seek 1ml
@22:21:21.52 delay 1 second
@22:21:22.55 AFV.select :holdingCoil
@22:21:22.65 delay 1 second
@22:21:23.67 ATV.dial :waste
@22:21:25.44 AS.reconfigure ASnorm
@22:21:25.50 AS.seek 0ml
@22:22:16.19 AFV.select :carrierSolution
@22:22:16.32 delay 2 seconds
@22:22:18.34 AS.reconfigure ASconfig
@22:22:18.40 AS.seek 0.5ml
@22:22:47.43 delay 1 second
@22:22:48.46 AFV.select :holdingCoil
@22:22:48.56 Building Slug
@22:22:48.57 ATV.dial :ARV
@22:22:50.31 delay 2 seconds
@22:22:52.33 ARV.dial :air1
@22:22:53.12 delay 2 seconds
@22:22:55.18 AS.reconfigure ASslow2
@22:22:55.25 AS.seek 0.506ml
@22:23:01.94 delay 2 seconds
@22:23:03.97 ARV.dial :MM3
@22:23:05.08 delay 2 seconds
@22:23:07.10 Adding MM3
@22:23:07.19 AS.seek 0.524ml
@22:23:25.79 delay 25 seconds
@22:23:50.82 ARV.dial :PP5
@22:23:51.23 Adding PP5
@22:23:51.24 delay 2 seconds
@22:23:53.32 AS.seek 0.53ml
@22:23:59.77 delay 10 seconds
@22:24:09.81 ARV.dial :air3
@22:24:10.23 adding ARV air
@22:24:10.24 delay 2 seconds
@22:24:12.32 AS.seek 0.536ml
@22:24:18.65 delay 5 seconds
@22:24:23.68 ARV.dial :carrier
@22:24:25.03 delay 7 seconds
@22:24:32.11 AS.seek 0.594ml
@22:25:30.99 delay 5 seconds
@22:25:36.02 ATV.dial :extSrc
@22:25:36.61 delay 2 seconds
@22:25:38.68 AS.seek 0.6ml
@22:25:44.82 delay 4 seconds
@22:25:48.85 ATV.dial ARV
@22:25:49.44 delay 2 seconds
@22:25:51.46 ARV.dial :carrier
@22:25:51.47 delay 2 seconds
@22:25:53.55 AS.seek 0.65ml
@22:26:44.29 delay 4 seconds
@22:26:48.32 ATV.dial PCR
@22:26:49.13 ASV.dial :waste1
@22:26:49.15 ARV.dial :air1
@22:26:49.56 Positioning Slug by 0.324ml
@22:26:49.65 AS.seek 0.326ml
@22:32:16.43 delay 5 seconds
@22:32:21.46 ATV.dial ARV
@22:32:22.25 ASV.dial :PCRlock
@22:32:22.67 delay 5 seconds
@22:32:27.69 Running ACOPP5 ProfileIPc thermocycling
@22:32:27.70 <PCR> PCR.program ProfileIPc
@22:32:27.83 ProfileIPc should complete in < 1:26:35
@22:32:27.84 PCR.run 1:53:13.75
@23:41:20.86 PCR.off
@23:41:21.42 <MFBPCR> PCR for ACOPP5 completed -- closed /var/log/esp/ACOPP521may0522h.pcr
@23:41:21.50 Cleaning PCR
@23:41:21.51 AFV.select :carrierSolution
@23:41:21.63 delay 2 seconds
@23:41:23.65 AS.reconfigure ASconfig
@23:41:23.71 AS.seek 0.5ml
@23:41:33.99 delay 1 second
@23:41:35.02 AFV.select :holdingCoil
@23:41:35.12 ASV.dial :waste1
@23:41:35.52 ATV.dial :air3
@23:41:36.86 AS.reconfigure ASslow1
@23:41:36.92 AS.seek 0.51ml
@23:41:39.19 delay 2 seconds
@23:41:41.22 ATV.dial :bleach
@23:41:41.86 AS.seek 0.81ml
@23:42:42.43 delay 3 seconds
@23:42:45.46 ATV.dial :air3
@23:42:46.06 delay 3 seconds
@23:42:49.12 AS.seek 0.835ml
@23:42:54.43 delay 3 seconds
@23:42:57.46 ATV.dial :bleach
@23:42:58.11 AS.seek 0.82ml
@23:43:01.41 delay 3 seconds
@23:43:04.44 ARV.dial :waste
@23:43:05.07 delay 3 seconds
@23:43:08.10 ATV.dial :ARV
@23:43:09.02 AS.reconfigure ASconfig
@23:43:09.08 AS.seek 0.47ml
@23:43:29.49 AFV.select :carrierSolution
@23:43:29.61 delay 2 seconds
@23:43:31.63 AS.seek 1ml
@23:44:02.38 delay 1 second
@23:44:03.41 AFV.select :holdingCoil
@23:44:03.51 delay 1 second
@23:44:04.53 AS.reconfigure ASfast
@23:44:04.59 AS.seek 0ml
@23:44:31.91 AFV.select :carrierSolution
@23:44:32.03 delay 2 seconds
@23:44:34.05 AS.reconfigure ASconfig
@23:44:34.11 AS.seek 1ml
@23:45:31.86 delay 1 second
@23:45:32.89 AFV.select :holdingCoil
@23:45:32.99 delay 1 second
@23:45:34.01 AS.reconfigure ASfast
@23:45:34.07 AS.seek 0ml
@23:46:01.41 AFV.select :carrierSolution
@23:46:01.53 delay 2 seconds
@23:46:03.55 AS.reconfigure ASconfig
@23:46:03.61 AS.seek 1ml
@23:47:01.36 delay 1 second
@23:47:02.39 AFV.select :holdingCoil
@23:47:02.49 delay 1 second
@23:47:03.51 AS.reconfigure ASfast
@23:47:03.57 AS.seek 0ml
@23:47:30.90 ARV.dial between carrier and air1
@23:47:31.44 ATV.dial :air3
@23:47:32.80 AS.reconfigure ASslow1
@23:47:32.86 AS.seek 0.01ml
@23:47:35.15 delay 2 seconds
@23:47:37.18 ATV.dial :bleach
@23:47:37.83 AS.seek 0.81ml
@23:50:18.96 delay 3 seconds
@23:50:21.99 ATV.dial :air3
@23:50:22.58 delay 3 seconds
@23:50:25.66 AS.seek 0.835ml
@23:50:30.94 delay 3 seconds
@23:50:34.29 ATV.dial :bleach
@23:50:35.00 AS.seek 0.82ml
@23:50:38.29 delay 3 seconds
@23:50:41.32 ATV.dial PCR
@23:50:41.73 AS.reconfigure ASconfig
@23:50:41.79 AS.seek 0ml
@23:51:29.18 PCR.program :bleachProfile
@23:51:29.25 PCR program should complete in < 2:45
@23:51:29.27 PCR.run 10:00
@23:54:25.05 PCR.off
@23:54:25.10 AFV.select :carrierSolution
@23:54:25.21 delay 2 seconds
@23:54:27.23 AS.seek 1ml
@23:55:24.97 delay 1 second
@23:55:26.00 AFV.select :holdingCoil
@23:55:26.10 delay 1 second
@23:55:27.12 AS.reconfigure ASfast
@23:55:27.18 AS.seek 0ml
@23:55:54.52 delay 3 seconds
@23:55:57.55 AFV.select :carrierSolution
@23:55:57.65 delay 2 seconds
@23:55:59.67 AS.reconfigure ASconfig
@23:55:59.74 AS.seek 1ml
@23:56:57.47 delay 1 second
@23:56:58.50 AFV.select :holdingCoil
@23:56:58.60 delay 1 second
@23:56:59.62 AS.reconfigure ASfast
@23:56:59.68 AS.seek 0ml
@23:57:27.01 delay 3 seconds
@23:57:30.04 AFV.select :carrierSolution
@23:57:30.14 delay 2 seconds
@23:57:32.16 AS.reconfigure ASconfig
@23:57:32.23 AS.seek 1ml
@23:58:29.97 delay 1 second
@23:58:31.00 AFV.select :holdingCoil
@23:58:31.10 delay 1 second
@23:58:32.12 AS.reconfigure ASfast
@23:58:32.18 AS.seek 0ml
@23:58:59.49 delay 3 seconds
@23:59:02.52 AFV.select :carrierSolution
@23:59:02.62 delay 2 seconds
@23:59:04.64 AS.reconfigure ASconfig
@23:59:04.70 AS.seek 1ml
@00:00:02.46CEST06-May-21 delay 1 second
@00:00:03.49 AFV.select :holdingCoil
@00:00:03.59 delay 1 second
@00:00:04.61 AS.reconfigure ASfast
@00:00:04.67 AS.seek 0ml
@00:00:32.02 delay 3 seconds
@00:00:35.05 AFV.select :carrierSolution
@00:00:35.15 delay 2 seconds
@00:00:37.17 AS.reconfigure ASconfig
@00:00:37.24 AS.seek 1ml
@00:01:34.99 delay 1 second
@00:01:36.02 AFV.select :holdingCoil
@00:01:36.12 delay 1 second
@00:01:37.14 AS.reconfigure ASnorm
@00:01:37.20 AS.seek 0ml
@00:02:27.91 <MAIN> AFV.select :carrierSolution
@00:02:28.01 delay 2 seconds
@00:02:30.03 AS.reconfigure ASconfig
@00:02:30.09 AS.seek 0.5ml
@00:02:59.16 delay 1 second
@00:03:00.19 AFV.select :holdingCoil
@00:03:00.29 ATV.dial :air1
@00:03:01.86 AS.reconfigure ASslow1
@00:03:01.91 AS.seek 0.52ml
@00:03:06.27 delay 5 seconds
@00:03:11.30 ATV.dial :extSrc
@00:03:13.08 AS.reconfigure ASslow2
@00:03:13.50 AS.seek 0.538ml
@00:03:32.02 delay 10 seconds
@00:03:42.05 ATV.dial :air1
@00:03:43.83 AS.reconfigure ASslow1
@00:03:43.88 AS.seek 0.568ml
@00:03:50.22 ATV.dial :waste
@00:03:50.80 AS.reconfigure ASconfig
@00:03:50.85 AS.seek 0ml
@00:04:23.94 <MFBPCR> Setting up PCR for ACOPP5 -- writing /var/log/esp/ACOPP521may0600h.pcr
@00:04:23.96 Building PCR Slug from extSrc with MM3 mix, PP5 primer
@00:04:23.99 Priming with carrier
@00:04:24.01 ATV.dial ARV
@00:04:25.57 ARV.dial :carrier
@00:04:25.92 AS.reconfigure ASnorm
@00:04:25.98 AS.seek 0.5ml
@00:04:51.47 AFV.select :carrierSolution
@00:04:51.60 delay 2 seconds
@00:04:53.62 AS.reconfigure ASconfig
@00:04:53.68 AS.seek 1ml
@00:05:22.66 delay 1 second
@00:05:23.69 AFV.select :holdingCoil
@00:05:23.79 delay 1 second
@00:05:24.81 ATV.dial :waste
@00:05:26.58 AS.reconfigure ASnorm
@00:05:26.64 AS.seek 0ml
@00:06:17.33 AFV.select :carrierSolution
@00:06:17.46 delay 2 seconds
@00:06:19.48 AS.reconfigure ASconfig
@00:06:19.54 AS.seek 0.5ml
@00:06:48.61 delay 1 second
@00:06:49.64 AFV.select :holdingCoil
@00:06:49.74 Building Slug
@00:06:49.75 ATV.dial :ARV
@00:06:51.50 delay 2 seconds
@00:06:53.52 ARV.dial :air1
@00:06:53.92 delay 2 seconds
@00:06:55.99 AS.reconfigure ASslow2
@00:06:56.05 AS.seek 0.506ml
@00:07:02.63 delay 2 seconds
@00:07:04.66 ARV.dial :MM3
@00:07:05.77 delay 2 seconds
@00:07:07.79 Adding MM3
@00:07:07.88 AS.seek 0.524ml
@00:07:26.33 delay 25 seconds
@00:07:51.36 ARV.dial :PP5
@00:07:51.78 Adding PP5
@00:07:51.79 delay 2 seconds
@00:07:53.87 AS.seek 0.53ml
@00:08:00.46 delay 10 seconds
@00:08:10.50 ARV.dial :air3
@00:08:10.92 adding ARV air
@00:08:10.93 delay 2 seconds
@00:08:13.01 AS.seek 0.536ml
@00:08:19.29 delay 5 seconds
@00:08:24.32 ARV.dial :carrier
@00:08:25.67 delay 7 seconds
@00:08:32.75 AS.seek 0.594ml
@00:09:31.47 delay 5 seconds
@00:09:36.50 ATV.dial :extSrc
@00:09:37.09 delay 2 seconds
@00:09:39.16 AS.seek 0.6ml
@00:09:45.43 delay 4 seconds
@00:09:49.46 ATV.dial ARV
@00:09:50.05 delay 2 seconds
@00:09:52.07 ARV.dial :carrier
@00:09:52.08 delay 2 seconds
@00:09:54.16 AS.seek 0.65ml
@00:10:44.91 delay 4 seconds
@00:10:48.94 ATV.dial PCR
@00:10:49.76 ASV.dial :waste1
@00:10:49.78 ARV.dial :air1
@00:10:50.20 Positioning Slug by 0.324ml
@00:10:50.29 AS.seek 0.326ml
@00:16:16.18 delay 5 seconds
@00:16:21.21 ATV.dial ARV
@00:16:22.02 ASV.dial :PCRlock
@00:16:22.44 delay 5 seconds
@00:16:27.46 Running ACOPP5 ProfileIPc thermocycling
@00:16:27.47 <PCR> PCR.program ProfileIPc
@00:16:27.59 ProfileIPc should complete in < 1:26:35
@00:16:27.60 PCR.run 1:53:13.75
@01:25:09.66 PCR.off
@01:25:10.21 <MFBPCR> PCR for ACOPP5 completed -- closed /var/log/esp/ACOPP521may0600h.pcr
@01:25:10.28 Cleaning PCR
@01:25:10.29 AFV.select :carrierSolution
@01:25:10.41 delay 2 seconds
@01:25:12.43 AS.reconfigure ASconfig
@01:25:12.49 AS.seek 0.5ml
@01:25:22.76 delay 1 second
@01:25:23.79 AFV.select :holdingCoil
@01:25:23.89 ASV.dial :waste1
@01:25:24.29 ATV.dial :air3
@01:25:25.64 AS.reconfigure ASslow1
@01:25:25.70 AS.seek 0.51ml
@01:25:28.11 delay 2 seconds
@01:25:30.14 ATV.dial :bleach
@01:25:30.78 AS.seek 0.81ml
@01:26:31.41 delay 3 seconds
@01:26:34.44 ATV.dial :air3
@01:26:35.02 delay 3 seconds
@01:26:38.10 AS.seek 0.835ml
@01:26:43.39 delay 3 seconds
@01:26:46.42 ATV.dial :bleach
@01:26:47.07 AS.seek 0.82ml
@01:26:50.38 delay 3 seconds
@01:26:53.41 ARV.dial :waste
@01:26:54.04 delay 3 seconds
@01:26:57.07 ATV.dial :ARV
@01:26:57.99 AS.reconfigure ASconfig
@01:26:58.05 AS.seek 0.47ml
@01:27:18.46 AFV.select :carrierSolution
@01:27:18.58 delay 2 seconds
@01:27:20.60 AS.seek 1ml
@01:27:51.75 delay 1 second
@01:27:52.77 AFV.select :holdingCoil
@01:27:52.87 delay 1 second
@01:27:53.89 AS.reconfigure ASfast
@01:27:53.95 AS.seek 0ml
@01:28:21.29 AFV.select :carrierSolution
@01:28:21.41 delay 2 seconds
@01:28:23.43 AS.reconfigure ASconfig
@01:28:23.49 AS.seek 1ml
@01:29:21.22 delay 1 second
@01:29:22.25 AFV.select :holdingCoil
@01:29:22.35 delay 1 second
@01:29:23.37 AS.reconfigure ASfast
@01:29:23.43 AS.seek 0ml
@01:29:50.77 AFV.select :carrierSolution
@01:29:50.89 delay 2 seconds
@01:29:52.91 AS.reconfigure ASconfig
@01:29:52.98 AS.seek 1ml
@01:30:50.72 delay 1 second
@01:30:51.75 AFV.select :holdingCoil
@01:30:51.85 delay 1 second
@01:30:52.87 AS.reconfigure ASfast
@01:30:52.93 AS.seek 0ml
@01:31:20.26 ARV.dial between carrier and air1
@01:31:20.80 ATV.dial :air3
@01:31:22.13 AS.reconfigure ASslow1
@01:31:22.19 AS.seek 0.01ml
@01:31:24.46 delay 2 seconds
@01:31:26.49 ATV.dial :bleach
@01:31:27.13 AS.seek 0.81ml
@01:34:08.26 delay 3 seconds
@01:34:11.29 ATV.dial :air3
@01:34:11.87 delay 3 seconds
@01:34:14.95 AS.seek 0.835ml
@01:34:20.33 delay 3 seconds
@01:34:23.36 ATV.dial :bleach
@01:34:24.00 AS.seek 0.82ml
@01:34:27.40 delay 3 seconds
@01:34:30.43 ATV.dial PCR
@01:34:30.84 AS.reconfigure ASconfig
@01:34:30.90 AS.seek 0ml
@01:35:18.32 PCR.program :bleachProfile
@01:35:18.39 PCR program should complete in < 2:45
@01:35:18.41 PCR.run 10:00
@01:38:14.19 PCR.off
@01:38:14.23 AFV.select :carrierSolution
@01:38:14.35 delay 2 seconds
@01:38:16.37 AS.seek 1ml
@01:39:14.12 delay 1 second
@01:39:15.15 AFV.select :holdingCoil
@01:39:15.25 delay 1 second
@01:39:16.27 AS.reconfigure ASfast
@01:39:16.33 AS.seek 0ml
@01:39:43.66 delay 3 seconds
@01:39:46.69 AFV.select :carrierSolution
@01:39:46.79 delay 2 seconds
@01:39:48.81 AS.reconfigure ASconfig
@01:39:48.87 AS.seek 1ml
@01:40:46.62 delay 1 second
@01:40:47.65 AFV.select :holdingCoil
@01:40:47.75 delay 1 second
@01:40:48.77 AS.reconfigure ASfast
@01:40:48.83 AS.seek 0ml
@01:41:16.16 delay 3 seconds
@01:41:19.19 AFV.select :carrierSolution
@01:41:19.68 delay 2 seconds
@01:41:21.70 AS.reconfigure ASconfig
@01:41:21.76 AS.seek 1ml
@01:42:19.55 delay 1 second
@01:42:20.58 AFV.select :holdingCoil
@01:42:20.68 delay 1 second
@01:42:21.70 AS.reconfigure ASfast
@01:42:21.76 AS.seek 0ml
@01:42:49.10 delay 3 seconds
@01:42:52.13 AFV.select :carrierSolution
@01:42:52.23 delay 2 seconds
@01:42:54.25 AS.reconfigure ASconfig
@01:42:54.31 AS.seek 1ml
@01:43:52.10 delay 1 second
@01:43:53.13 AFV.select :holdingCoil
@01:43:53.23 delay 1 second
@01:43:54.25 AS.reconfigure ASfast
@01:43:54.31 AS.seek 0ml
@01:44:21.63 delay 3 seconds
@01:44:24.66 AFV.select :carrierSolution
@01:44:24.76 delay 2 seconds
@01:44:26.78 AS.reconfigure ASconfig
@01:44:26.85 AS.seek 1ml
@01:45:24.58 delay 1 second
@01:45:25.61 AFV.select :holdingCoil
@01:45:25.71 delay 1 second
@01:45:26.73 AS.reconfigure ASnorm
@01:45:26.79 AS.seek 0ml
@01:46:17.49 <MAIN> AFV.select :carrierSolution
@01:46:17.59 delay 2 seconds
@01:46:19.61 AS.reconfigure ASconfig
@01:46:19.66 AS.seek 0.5ml
@01:46:48.68 delay 1 second
@01:46:49.71 AFV.select :holdingCoil
@01:46:49.81 ATV.dial :air1
@01:46:51.38 AS.reconfigure ASslow2
@01:46:51.43 AS.seek 0.506ml
@01:46:57.90 ATV.dial :extSrc
@01:46:59.68 AS.seek 0.512ml
@01:47:06.18 delay 5 seconds
@01:47:11.21 ATV.dial :air1
@01:47:13.18 AS.reconfigure ASslow1
@01:47:13.23 AS.seek 0.542ml
@01:47:19.54 ATV.dial :waste
@01:47:20.14 AS.reconfigure ASconfig
@01:47:20.19 AS.seek 0ml
@01:47:51.79 <MFBPCR> Setting up PCR for ACOPP5 -- writing /var/log/esp/ACOPP521may0601h.pcr
@01:47:51.82 Building PCR Slug from extSrc with MM3 mix, PP5 primer
@01:47:51.84 Priming with carrier
@01:47:51.85 ATV.dial ARV
@01:47:53.41 ARV.dial :carrier
@01:47:53.77 AS.reconfigure ASnorm
@01:47:53.83 AS.seek 0.5ml
@01:48:19.43 AFV.select :carrierSolution
@01:48:19.56 delay 2 seconds
@01:48:21.58 AS.reconfigure ASconfig
@01:48:21.64 AS.seek 1ml
@01:48:50.65 delay 1 second
@01:48:51.68 AFV.select :holdingCoil
@01:48:51.78 delay 1 second
@01:48:52.80 ATV.dial :waste
@01:48:54.56 AS.reconfigure ASnorm
@01:48:54.62 AS.seek 0ml
@01:49:45.30 AFV.select :carrierSolution
@01:49:45.42 delay 2 seconds
@01:49:47.44 AS.reconfigure ASconfig
@01:49:47.50 AS.seek 0.5ml
@01:50:16.57 delay 1 second
@01:50:17.60 AFV.select :holdingCoil
@01:50:17.70 Building Slug
@01:50:17.71 ATV.dial :ARV
@01:50:19.45 delay 2 seconds
@01:50:21.47 ARV.dial :air1
@01:50:21.88 delay 2 seconds
@01:50:23.95 AS.reconfigure ASslow2
@01:50:24.01 AS.seek 0.506ml
@01:50:30.35 delay 2 seconds
@01:50:32.38 ARV.dial :MM3
@01:50:33.48 delay 2 seconds
@01:50:35.51 Adding MM3
@01:50:35.58 AS.seek 0.524ml
@01:50:53.80 delay 25 seconds
@01:51:18.83 ARV.dial :PP5
@01:51:19.25 Adding PP5
@01:51:19.26 delay 2 seconds
@01:51:21.34 AS.seek 0.53ml
@01:51:27.52 delay 10 seconds
@01:51:37.56 ARV.dial :air3
@01:51:37.97 adding ARV air
@01:51:37.98 delay 2 seconds
@01:51:40.06 AS.seek 0.536ml
@01:51:46.83 delay 5 seconds
@01:51:51.86 ARV.dial :carrier
@01:51:53.20 delay 7 seconds
@01:52:00.28 AS.seek 0.594ml
@01:52:59.37 delay 5 seconds
@01:53:04.40 ATV.dial :extSrc
@01:53:05.00 delay 2 seconds
@01:53:07.07 AS.seek 0.6ml
@01:53:13.32 delay 4 seconds
@01:53:17.35 ATV.dial ARV
@01:53:18.03 delay 2 seconds
@01:53:20.05 ARV.dial :carrier
@01:53:20.06 delay 2 seconds
@01:53:22.14 AS.seek 0.65ml
@01:54:12.58 delay 4 seconds
@01:54:16.61 ATV.dial PCR
@01:54:17.41 ASV.dial :waste1
@01:54:17.43 ARV.dial :air1
@01:54:17.87 Positioning Slug by 0.324ml
@01:54:17.96 AS.seek 0.326ml
@01:59:44.18 delay 5 seconds
@01:59:49.21 ATV.dial ARV
@01:59:50.02 ASV.dial :PCRlock
@01:59:50.44 delay 5 seconds
@01:59:55.46 Running ACOPP5 ProfileIPc thermocycling
@01:59:55.48 <PCR> PCR.program ProfileIPc
@01:59:55.99 ProfileIPc should complete in < 1:26:35
@01:59:56.00 PCR.run 1:53:13.75
@03:08:38.76 PCR.off
@03:08:39.32 <MFBPCR> PCR for ACOPP5 completed -- closed /var/log/esp/ACOPP521may0601h.pcr
@03:08:39.74 Cleaning PCR
@03:08:39.75 AFV.select :carrierSolution
@03:08:39.88 delay 2 seconds
@03:08:41.90 AS.reconfigure ASconfig
@03:08:41.96 AS.seek 0.5ml
@03:08:52.24 delay 1 second
@03:08:53.27 AFV.select :holdingCoil
@03:08:53.37 ASV.dial :waste1
@03:08:53.76 ATV.dial :air3
@03:08:55.11 AS.reconfigure ASslow1
@03:08:55.17 AS.seek 0.51ml
@03:08:57.44 delay 2 seconds
@03:08:59.47 ATV.dial :bleach
@03:09:00.11 AS.seek 0.81ml
@03:10:00.69 delay 3 seconds
@03:10:03.72 ATV.dial :air3
@03:10:04.31 delay 3 seconds
@03:10:07.38 AS.seek 0.835ml
@03:10:12.66 delay 3 seconds
@03:10:15.69 ATV.dial :bleach
@03:10:16.33 AS.seek 0.82ml
@03:10:19.62 delay 3 seconds
@03:10:22.65 ARV.dial :waste
@03:10:23.29 delay 3 seconds
@03:10:26.32 ATV.dial :ARV
@03:10:27.24 AS.reconfigure ASconfig
@03:10:27.30 AS.seek 0.47ml
@03:10:47.69 AFV.select :carrierSolution
@03:10:47.81 delay 2 seconds
@03:10:49.83 AS.seek 1ml
@03:11:20.62 delay 1 second
@03:11:21.65 AFV.select :holdingCoil
@03:11:21.75 delay 1 second
@03:11:22.77 AS.reconfigure ASfast
@03:11:22.84 AS.seek 0ml
@03:11:50.18 AFV.select :carrierSolution
@03:11:50.30 delay 2 seconds
@03:11:52.32 AS.reconfigure ASconfig
@03:11:52.38 AS.seek 1ml
@03:12:50.12 delay 1 second
@03:12:51.15 AFV.select :holdingCoil
@03:12:51.25 delay 1 second
@03:12:52.27 AS.reconfigure ASfast
@03:12:52.33 AS.seek 0ml
@03:13:19.66 AFV.select :carrierSolution
@03:13:19.78 delay 2 seconds
@03:13:21.80 AS.reconfigure ASconfig
@03:13:21.87 AS.seek 1ml
@03:14:19.62 delay 1 second
@03:14:20.65 AFV.select :holdingCoil
@03:14:20.75 delay 1 second
@03:14:21.77 AS.reconfigure ASfast
@03:14:21.83 AS.seek 0ml
@03:14:49.16 ARV.dial between carrier and air1
@03:14:49.69 ATV.dial :air3
@03:14:51.04 AS.reconfigure ASslow1
@03:14:51.10 AS.seek 0.01ml
@03:14:53.52 delay 2 seconds
@03:14:55.55 ATV.dial :bleach
@03:14:56.19 AS.seek 0.81ml
@03:17:37.34 delay 3 seconds
@03:17:40.37 ATV.dial :air3
@03:17:40.96 delay 3 seconds
@03:17:44.03 AS.seek 0.835ml
@03:17:49.44 delay 3 seconds
@03:17:52.47 ATV.dial :bleach
@03:17:53.11 AS.seek 0.82ml
@03:17:56.37 delay 3 seconds
@03:17:59.40 ATV.dial PCR
@03:17:59.81 AS.reconfigure ASconfig
@03:17:59.86 AS.seek 0ml
@03:18:47.27 PCR.program :bleachProfile
@03:18:47.34 PCR program should complete in < 2:45
@03:18:47.36 PCR.run 10:00
@03:21:43.51 PCR.off
@03:21:43.56 AFV.select :carrierSolution
@03:21:43.67 delay 2 seconds
@03:21:45.69 AS.seek 1ml
@03:22:43.44 delay 1 second
@03:22:44.47 AFV.select :holdingCoil
@03:22:44.57 delay 1 second
@03:22:45.59 AS.reconfigure ASfast
@03:22:45.65 AS.seek 0ml
@03:23:12.98 delay 3 seconds
@03:23:16.01 AFV.select :carrierSolution
@03:23:16.11 delay 2 seconds
@03:23:18.13 AS.reconfigure ASconfig
@03:23:18.20 AS.seek 1ml
@03:24:15.94 delay 1 second
@03:24:16.97 AFV.select :holdingCoil
@03:24:17.07 delay 1 second
@03:24:18.09 AS.reconfigure ASfast
@03:24:18.15 AS.seek 0ml
@03:24:45.49 delay 3 seconds
@03:24:48.52 AFV.select :carrierSolution
@03:24:48.62 delay 2 seconds
@03:24:50.64 AS.reconfigure ASconfig
@03:24:50.71 AS.seek 1ml
@03:25:48.46 delay 1 second
@03:25:49.49 AFV.select :holdingCoil
@03:25:49.59 delay 1 second
@03:25:50.61 AS.reconfigure ASfast
@03:25:50.67 AS.seek 0ml
@03:26:18.01 delay 3 seconds
@03:26:21.04 AFV.select :carrierSolution
@03:26:21.14 delay 2 seconds
@03:26:23.16 AS.reconfigure ASconfig
@03:26:23.23 AS.seek 1ml
@03:27:21.02 delay 1 second
@03:27:22.05 AFV.select :holdingCoil
@03:27:22.15 delay 1 second
@03:27:23.17 AS.reconfigure ASfast
@03:27:23.23 AS.seek 0ml
@03:27:50.57 delay 3 seconds
@03:27:53.60 AFV.select :carrierSolution
@03:27:53.70 delay 2 seconds
@03:27:55.72 AS.reconfigure ASconfig
@03:27:55.79 AS.seek 1ml
@03:28:53.54 delay 1 second
@03:28:54.57 AFV.select :holdingCoil
@03:28:54.67 delay 1 second
@03:28:55.69 AS.reconfigure ASnorm
@03:28:55.75 AS.seek 0ml
@03:29:46.47 <MAIN> deconEluateRecoveryLine extSrc
@03:29:46.48 ATV.dial :waste
@03:29:47.78 AS.seek 0ml
@03:29:47.85 ATV.dial :air3
@03:29:48.72 AS.reconfigure ASslow1
@03:29:48.77 AS.seek 0.01ml
@03:29:51.23 delay 2 seconds
@03:29:53.26 ATV.dial :bleach
@03:29:53.72 AS.seek 0.31ml
@03:30:54.34 delay 3 seconds
@03:30:57.37 ATV.dial :air3
@03:30:57.94 delay 3 seconds
@03:31:01.02 AS.seek 0.335ml
@03:31:06.35 delay 3 seconds
@03:31:09.38 ATV.dial :bleach
@03:31:10.00 AS.seek 0.32ml
@03:31:13.32 delay 3 seconds
@03:31:16.35 ATV.dial :extSrc
@03:31:16.99 AS.seek 0ml
@03:32:21.62 AFV.select :carrierSolution
@03:32:21.74 delay 2 seconds
@03:32:23.76 AS.reconfigure ASconfig
@03:32:23.81 AS.seek 0.5ml
@03:32:52.85 delay 1 second
@03:32:53.88 AFV.select :holdingCoil
@03:32:53.98 ATV.dial :extSrc
@03:32:54.00 AS.reconfigure ASslow1
@03:32:54.06 AS.seek 0ml
@03:34:34.99 AFV.select :carrierSolution
@03:34:35.11 delay 2 seconds
@03:34:37.13 AS.reconfigure ASconfig
@03:34:37.18 AS.seek 0.5ml
@03:35:06.19 delay 1 second
@03:35:07.22 AFV.select :holdingCoil
@03:35:07.32 ATV.dial :extSrc
@03:35:07.34 AS.reconfigure ASslow1
@03:35:07.40 AS.seek 0ml
@03:36:48.24 AFV.select :carrierSolution
@03:36:48.36 delay 2 seconds
@03:36:50.38 AS.reconfigure ASconfig
@03:36:50.43 AS.seek 0.5ml
@03:37:19.48 delay 1 second
@03:37:20.51 AFV.select :holdingCoil
@03:37:20.61 ATV.dial :extSrc
@03:37:20.63 AS.reconfigure ASslow1
@03:37:20.69 AS.seek 0ml
@03:39:01.52 AFV.select :carrierSolution
@03:39:01.64 delay 2 seconds
@03:39:03.66 AS.reconfigure ASconfig
@03:39:03.71 AS.seek 0.5ml
@03:39:32.76 delay 1 second
@03:39:33.79 AFV.select :holdingCoil
@03:39:33.89 ATV.dial :extSrc
@03:39:33.91 AS.reconfigure ASslow1
@03:39:33.97 AS.seek 0ml
@03:41:14.79 ATV.dial :air2
@03:41:16.18 AS.reconfigure ASslow0
@03:41:16.23 AS.seek 0.5ml
@03:42:06.87 ATV.dial :extSrc
@03:42:08.43 AS.seek 0.02ml
@03:42:57.04 delay 1 second
@03:42:58.07 ATV.dial :waste
@03:42:59.41 AS.reconfigure ASconfig
@03:42:59.46 AS.seek 0ml
@03:43:00.90 deconEluateRecoveryLine extSrc
@03:43:00.91 ATV.dial :waste
@03:43:00.94 AS.reconfigure ASnorm
@03:43:01.00 AS.seek 0ml
@03:43:01.48 ATV.dial :air3
@03:43:02.36 AS.reconfigure ASslow1
@03:43:02.41 AS.seek 0.01ml
@03:43:04.82 delay 2 seconds
@03:43:06.85 ATV.dial :bleach
@03:43:07.33 AS.seek 0.31ml
@03:44:08.08 delay 3 seconds
@03:44:11.11 ATV.dial :air3
@03:44:11.70 delay 3 seconds
@03:44:14.77 AS.seek 0.335ml
@03:44:20.08 delay 3 seconds
@03:44:23.11 ATV.dial :bleach
@03:44:23.79 AS.seek 0.32ml
@03:44:27.10 delay 3 seconds
@03:44:30.13 ATV.dial :extSrc
@03:44:30.77 AS.seek 0ml
@03:45:35.40 AFV.select :carrierSolution
@03:45:35.52 delay 2 seconds
@03:45:37.54 AS.reconfigure ASconfig
@03:45:37.60 AS.seek 0.5ml
@03:46:06.63 delay 1 second
@03:46:07.66 AFV.select :holdingCoil
@03:46:07.76 ATV.dial :extSrc
@03:46:07.78 AS.reconfigure ASslow1
@03:46:07.84 AS.seek 0ml
@03:47:48.79 AFV.select :carrierSolution
@03:47:48.91 delay 2 seconds
@03:47:50.94 AS.reconfigure ASconfig
@03:47:50.99 AS.seek 0.5ml
@03:48:20.01 delay 1 second
@03:48:21.04 AFV.select :holdingCoil
@03:48:21.14 ATV.dial :extSrc
@03:48:21.16 AS.reconfigure ASslow1
@03:48:21.23 AS.seek 0ml
@03:50:02.16 AFV.select :carrierSolution
@03:50:02.28 delay 2 seconds
@03:50:04.30 AS.reconfigure ASconfig
@03:50:04.35 AS.seek 0.5ml
@03:50:33.38 delay 1 second
@03:50:34.41 AFV.select :holdingCoil
@03:50:34.51 ATV.dial :extSrc
@03:50:34.53 AS.reconfigure ASslow1
@03:50:34.59 AS.seek 0ml
@03:52:15.41 AFV.select :carrierSolution
@03:52:15.53 delay 2 seconds
@03:52:17.55 AS.reconfigure ASconfig
@03:52:17.61 AS.seek 0.5ml
@03:52:46.65 delay 1 second
@03:52:47.68 AFV.select :holdingCoil
@03:52:47.78 ATV.dial :extSrc
@03:52:47.80 AS.reconfigure ASslow1
@03:52:47.86 AS.seek 0ml
@03:54:28.68 ATV.dial :air2
@03:54:30.08 AS.reconfigure ASslow0
@03:54:30.13 AS.seek 0.5ml
@03:55:20.77 ATV.dial :extSrc
@03:55:22.32 AS.seek 0.02ml
@03:56:10.95 delay 1 second
@03:56:11.98 ATV.dial :waste
@03:56:13.31 AS.reconfigure ASconfig
@03:56:13.37 AS.seek 0ml
@03:56:14.79 deconEluateRecoveryLine extSrc
@03:56:14.81 ATV.dial :waste
@03:56:14.83 AS.reconfigure ASnorm
@03:56:14.89 AS.seek 0ml
@03:56:15.02 ATV.dial :air3
@03:56:15.90 AS.reconfigure ASslow1
@03:56:15.95 AS.seek 0.01ml
@03:56:18.27 delay 2 seconds
@03:56:20.30 ATV.dial :bleach
@03:56:20.77 AS.seek 0.31ml
@03:57:21.45 delay 3 seconds
@03:57:24.48 ATV.dial :air3
@03:57:25.05 delay 3 seconds
@03:57:28.12 AS.seek 0.335ml
@03:57:33.49 delay 3 seconds
@03:57:36.52 ATV.dial :bleach
@03:57:37.16 AS.seek 0.32ml
@03:57:40.44 delay 3 seconds
@03:57:43.47 ATV.dial :extSrc
@03:57:44.10 AS.seek 0ml
@03:58:48.85 AFV.select :carrierSolution
@03:58:48.97 delay 2 seconds
@03:58:50.99 AS.reconfigure ASconfig
@03:58:51.04 AS.seek 0.5ml
@03:59:20.05 delay 1 second
@03:59:21.08 AFV.select :holdingCoil
@03:59:21.18 ATV.dial :extSrc
@03:59:21.20 AS.reconfigure ASslow1
@03:59:21.26 AS.seek 0ml
@04:01:02.19 AFV.select :carrierSolution
@04:01:02.31 delay 2 seconds
@04:01:04.33 AS.reconfigure ASconfig
@04:01:04.38 AS.seek 0.5ml
@04:01:33.40 delay 1 second
@04:01:34.43 AFV.select :holdingCoil
@04:01:34.53 ATV.dial :extSrc
@04:01:34.55 AS.reconfigure ASslow1
@04:01:34.62 AS.seek 0ml
@04:03:15.45 AFV.select :carrierSolution
@04:03:15.57 delay 2 seconds
@04:03:17.59 AS.reconfigure ASconfig
@04:03:17.64 AS.seek 0.5ml
@04:03:46.68 delay 1 second
@04:03:47.71 AFV.select :holdingCoil
@04:03:47.81 ATV.dial :extSrc
@04:03:47.83 AS.reconfigure ASslow1
@04:03:47.90 AS.seek 0ml
@04:05:28.71 AFV.select :carrierSolution
@04:05:28.83 delay 2 seconds
@04:05:30.85 AS.reconfigure ASconfig
@04:05:30.90 AS.seek 0.5ml
@04:05:59.93 delay 1 second
@04:06:00.96 AFV.select :holdingCoil
@04:06:01.06 ATV.dial :extSrc
@04:06:01.08 AS.reconfigure ASslow1
@04:06:01.14 AS.seek 0ml
@04:07:42.09 ATV.dial :air2
@04:07:43.49 AS.reconfigure ASslow0
@04:07:43.54 AS.seek 0.5ml
@04:08:34.16 ATV.dial :extSrc
@04:08:35.71 AS.seek 0.02ml
@04:09:24.29 delay 1 second
@04:09:25.32 ATV.dial :waste
@04:09:26.67 AS.reconfigure ASconfig
@04:09:26.72 AS.seek 0ml
@04:09:28.21 <mwj> Session Ended
@12:58:20.39CEST06-May-21 <MAIN> ESP2 v3.30 I2C Gateway v3.06 for core bus
@12:58:22.52 
Configuration for mer 7/20/20 brent@mbari.org
/home/esp/esp2/type/shallow/mer/configure.rb
@12:58:22.61 
Configuration for MFB3 by preston@mbari.org
/home/esp/esp2/type/mfb/MFB3/configure.rb
@12:58:27.82 Polling Can every 10:00
@12:58:29.59 CS.configure :default
@12:58:29.66 CS.hold
@12:58:29.77 CS.configure :default
@12:58:29.94 PS.configure :default
@12:58:30.10 SS.configure :default
@12:58:30.18 SS.hold
@12:58:30.26 SS.configure :default
@12:58:30.38 CC.configure :default
@12:58:30.41 CC.hold
@12:58:30.46 CC.configure :default
@12:58:30.53 PC.configure :default
@12:58:30.57 PC.hold
@12:58:30.62 PC.configure :default
@12:58:30.72 Forearm.configure :loose
@12:58:30.75 Forearm.hold
@12:58:30.79 Forearm.configure :loose
@12:58:30.87 Elbow.configure :loose
@12:58:30.91 Elbow.hold
@12:58:30.95 Elbow.configure :loose
@12:58:31.05 SC.configure :loose
@12:58:31.09 SC.hold
@12:58:31.15 SC.configure :loose
@12:58:31.23 SE.configure :default
@12:58:31.27 SE.hold
@12:58:31.32 SE.configure :default
@12:58:31.36 CH.coast
@12:58:31.40 CH.configure :default
@12:58:31.43 PH.coast
@12:58:31.48 PH.configure :default
@12:58:31.58 SPE.coast
@12:58:31.66 SPE.configure SPEconfig
@12:58:31.80 AS.configure ASconfig
@12:58:31.88 AS.hold
@12:58:31.97 AS.configure ASconfig
@12:58:32.06 PCR.off
@12:58:32.11 PCR.pidtemp= 50,0.1,0,300,25,25
@12:58:32.16 PCR.caltemp= 2.5051,0.0372,0
@12:58:32.20 All dwarf controllers running firmware version 3.75
@12:58:32.49 Cleanly exited at 04:09:28CEST06-May-21
@12:58:32.52 Loading /home/esp/esp2/mission/stdMER2017.rb mission
@12:58:33.06 AFV.select :carrierSolution
@12:58:33.16 delay 2 seconds
@12:58:34.30 <mwj> Session Begun
@12:58:35.18 <MAIN> AS.seek 0.5ml
@12:59:04.20 delay 1 second
@12:59:05.23 AFV.select :holdingCoil
@12:59:05.33 ATV.dial :extSrc
@12:59:06.88 AS.reconfigure ASslow1
@12:59:06.93 AS.seek 0.53ml
@12:59:13.23 delay 5 seconds
@12:59:18.31 AS.reconfigure ASslow2
@12:59:18.37 AS.seek 0.54ml
@12:59:28.86 delay 20 seconds
@12:59:48.93 AS.reconfigure ASslow1
@12:59:48.99 AS.seek 0.57ml
@12:59:55.29 delay 5 seconds
@13:00:00.37 AS.reconfigure ASslow2
@13:00:00.43 AS.seek 0.58ml
@13:00:10.68 delay 20 seconds
@13:00:30.75 AS.reconfigure ASslow1
@13:00:30.81 AS.seek 0.61ml
@13:00:37.20 delay 5 seconds
@13:00:42.28 AS.reconfigure ASslow2
@13:00:42.33 AS.seek 0.62ml
@13:00:53.09 delay 20 seconds
@13:01:13.16 AS.reconfigure ASslow1
@13:01:13.22 AS.seek 0.65ml
@13:01:19.57 delay 5 seconds
@13:01:24.65 AS.reconfigure ASslow2
@13:01:24.70 AS.seek 0.66ml
@13:01:35.11 delay 20 seconds
@13:01:55.14 AS.reconfigure ASslow1
@13:01:55.19 AS.seek 0.705ml
@13:02:04.50 delay 10 seconds
@13:02:14.53 ATV.dial :air1
@13:02:16.70 AS.seek 0.725ml
@13:02:21.04 delay 3 seconds
@13:02:24.07 ATV.dial :waste
@13:02:24.66 AS.reconfigure ASslow0
@13:02:24.71 AS.seek 0ml
@13:03:38.10 <MFBPCR> Setting up PCR for AOPP1 -- writing /var/log/esp/AOPP121may0613h.pcr
@13:03:38.12 Building PCR Slug from extSrc with MM1 mix, PP1 primer
@13:03:38.15 Priming with carrier
@13:03:38.16 ATV.dial ARV
@13:03:39.72 ARV.dial :carrier
@13:03:40.09 AS.reconfigure ASnorm
@13:03:40.15 AS.seek 0.5ml
@13:04:05.67 AFV.select :carrierSolution
@13:04:05.79 delay 2 seconds
@13:04:07.81 AS.reconfigure ASconfig
@13:04:07.88 AS.seek 1ml
@13:04:36.89 delay 1 second
@13:04:37.92 AFV.select :holdingCoil
@13:04:38.02 delay 1 second
@13:04:39.04 ATV.dial :waste
@13:04:40.79 AS.reconfigure ASnorm
@13:04:40.85 AS.seek 0ml
@13:05:31.54 AFV.select :carrierSolution
@13:05:31.66 delay 2 seconds
@13:05:33.68 AS.reconfigure ASconfig
@13:05:33.74 AS.seek 0.5ml
@13:06:02.78 delay 1 second
@13:06:03.81 AFV.select :holdingCoil
@13:06:03.91 Building Slug
@13:06:03.92 ATV.dial :ARV
@13:06:05.66 delay 2 seconds
@13:06:07.68 ARV.dial :air1
@13:06:08.10 delay 2 seconds
@13:06:10.17 AS.reconfigure ASslow2
@13:06:10.24 AS.seek 0.506ml
@13:06:16.73 delay 2 seconds
@13:06:18.76 ARV.dial :MM1
@13:06:19.39 delay 2 seconds
@13:06:21.42 Adding MM1
@13:06:21.50 AS.seek 0.524ml
@13:06:39.81 delay 25 seconds
@13:07:04.84 ARV.dial :PP1
@13:07:05.26 Adding PP1
@13:07:05.27 delay 2 seconds
@13:07:07.34 AS.seek 0.53ml
@13:07:13.54 delay 10 seconds
@13:07:23.57 ARV.dial :air1
@13:07:23.99 adding ARV air
@13:07:24.00 delay 2 seconds
@13:07:26.07 AS.seek 0.536ml
@13:07:32.39 delay 5 seconds
@13:07:37.42 ARV.dial :carrier
@13:07:37.85 delay 7 seconds
@13:07:44.91 AS.seek 0.594ml
@13:08:43.42 delay 5 seconds
@13:08:48.45 ATV.dial :extSrc
@13:08:49.04 delay 2 seconds
@13:08:51.12 AS.seek 0.6ml
@13:08:57.31 delay 4 seconds
@13:09:01.34 ATV.dial ARV
@13:09:01.90 delay 2 seconds
@13:09:03.93 ARV.dial :carrier
@13:09:03.94 delay 2 seconds
@13:09:06.01 AS.seek 0.65ml
@13:09:56.45 delay 4 seconds
@13:10:00.48 ATV.dial PCR
@13:10:01.28 ASV.dial :waste1
@13:10:01.37 ARV.dial :air1
@13:10:01.82 Positioning Slug by 0.324ml
@13:10:01.91 AS.seek 0.326ml
@13:15:28.32 delay 5 seconds
@13:15:33.35 ATV.dial ARV
@13:15:34.15 ASV.dial :PCRlock
@13:15:34.54 delay 5 seconds
@13:15:39.57 Running AOPP1 Profile60CIQ1 thermocycling
@13:15:39.58 <PCR> PCR.program Profile60CIQ1
@13:15:39.71 Profile60CIQ1 should complete in < 1:56:15
@13:15:39.72 PCR.run 2:30:18.75
@14:54:53.70 PCR.off
@14:54:54.26 <MFBPCR> Running AOPP1 MeltProfile60 thermocycling
@14:54:54.27 <PCR> PCR.program MeltProfile60
@14:54:54.38 MeltProfile60 should complete in < 11:55
@14:54:54.39 PCR.run 19:53.75
@15:02:26.33 PCR.off
@15:02:27.23 <MFBPCR> PCR for AOPP1 completed -- closed /var/log/esp/AOPP121may0613h.pcr
@15:02:27.31 Cleaning PCR
@15:02:27.32 AFV.select :carrierSolution
@15:02:27.44 delay 2 seconds
@15:02:29.46 AS.reconfigure ASconfig
@15:02:29.51 AS.seek 0.5ml
@15:02:39.82 delay 1 second
@15:02:40.85 AFV.select :holdingCoil
@15:02:40.95 ASV.dial :waste1
@15:02:41.34 ATV.dial :air3
@15:02:42.70 AS.reconfigure ASslow1
@15:02:42.76 AS.seek 0.51ml
@15:02:45.11 delay 2 seconds
@15:02:47.14 ATV.dial :bleach
@15:02:47.79 AS.seek 0.81ml
@15:03:48.39 delay 3 seconds
@15:03:51.42 ATV.dial :air3
@15:03:52.00 delay 3 seconds
@15:03:55.07 AS.seek 0.835ml
@15:04:00.36 delay 3 seconds
@15:04:03.39 ATV.dial :bleach
@15:04:04.02 AS.seek 0.82ml
@15:04:07.35 delay 3 seconds
@15:04:10.38 ARV.dial :waste
@15:04:11.03 delay 3 seconds
@15:04:14.06 ATV.dial :ARV
@15:04:15.09 AS.reconfigure ASconfig
@15:04:15.14 AS.seek 0.47ml
@15:04:35.56 AFV.select :carrierSolution
@15:04:35.68 delay 2 seconds
@15:04:37.70 AS.seek 1ml
@15:05:08.48 delay 1 second
@15:05:09.51 AFV.select :holdingCoil
@15:05:09.61 delay 1 second
@15:05:10.63 AS.reconfigure ASfast
@15:05:10.68 AS.seek 0ml
@15:05:38.01 AFV.select :carrierSolution
@15:05:38.13 delay 2 seconds
@15:05:40.15 AS.reconfigure ASconfig
@15:05:40.20 AS.seek 1ml
@15:06:37.96 delay 1 second
@15:06:38.99 AFV.select :holdingCoil
@15:06:39.09 delay 1 second
@15:06:40.11 AS.reconfigure ASfast
@15:06:40.16 AS.seek 0ml
@15:07:07.48 AFV.select :carrierSolution
@15:07:07.59 delay 2 seconds
@15:07:09.61 AS.reconfigure ASconfig
@15:07:09.66 AS.seek 1ml
@15:08:07.42 delay 1 second
@15:08:08.45 AFV.select :holdingCoil
@15:08:08.55 delay 1 second
@15:08:09.57 AS.reconfigure ASfast
@15:08:09.62 AS.seek 0ml
@15:08:36.92 ARV.dial between carrier and air1
@15:08:37.46 ATV.dial :air3
@15:08:38.81 AS.reconfigure ASslow1
@15:08:38.87 AS.seek 0.01ml
@15:08:41.17 delay 2 seconds
@15:08:43.20 ATV.dial :bleach
@15:08:43.85 AS.seek 0.81ml
@15:11:25.03 delay 3 seconds
@15:11:28.06 ATV.dial :air3
@15:11:28.65 delay 3 seconds
@15:11:31.71 AS.seek 0.835ml
@15:11:37.08 delay 3 seconds
@15:11:40.11 ATV.dial :bleach
@15:11:40.74 AS.seek 0.82ml
@15:11:44.14 delay 3 seconds
@15:11:47.55 ATV.dial PCR
@15:11:47.97 AS.reconfigure ASconfig
@15:11:48.02 AS.seek 0ml
@15:12:35.45 PCR.program :bleachProfile
@15:12:35.53 PCR program should complete in < 2:45
@15:12:35.54 PCR.run 10:00
@15:15:31.33 PCR.off
@15:15:31.37 AFV.select :carrierSolution
@15:15:31.48 delay 2 seconds
@15:15:33.50 AS.seek 1ml
@15:16:31.23 delay 1 second
@15:16:32.26 AFV.select :holdingCoil
@15:16:32.36 delay 1 second
@15:16:33.38 AS.reconfigure ASfast
@15:16:33.43 AS.seek 0ml
@15:17:00.76 delay 3 seconds
@15:17:03.79 AFV.select :carrierSolution
@15:17:03.89 delay 2 seconds
@15:17:05.91 AS.reconfigure ASconfig
@15:17:05.97 AS.seek 1ml
@15:18:03.73 delay 1 second
@15:18:04.76 AFV.select :holdingCoil
@15:18:04.86 delay 1 second
@15:18:05.88 AS.reconfigure ASfast
@15:18:05.93 AS.seek 0ml
@15:18:33.27 delay 3 seconds
@15:18:36.30 AFV.select :carrierSolution
@15:18:36.40 delay 2 seconds
@15:18:38.42 AS.reconfigure ASconfig
@15:18:38.47 AS.seek 1ml
@15:19:36.23 delay 1 second
@15:19:37.26 AFV.select :holdingCoil
@15:19:37.36 delay 1 second
@15:19:38.38 AS.reconfigure ASfast
@15:19:38.43 AS.seek 0ml
@15:20:05.76 delay 3 seconds
@15:20:08.79 AFV.select :carrierSolution
@15:20:08.89 delay 2 seconds
@15:20:10.91 AS.reconfigure ASconfig
@15:20:10.98 AS.seek 1ml
@15:21:08.71 delay 1 second
@15:21:09.74 AFV.select :holdingCoil
@15:21:09.84 delay 1 second
@15:21:10.86 AS.reconfigure ASfast
@15:21:10.91 AS.seek 0ml
@15:21:38.23 delay 3 seconds
@15:21:41.26 AFV.select :carrierSolution
@15:21:41.36 delay 2 seconds
@15:21:43.38 AS.reconfigure ASconfig
@15:21:43.46 AS.seek 1ml
@15:22:41.21 delay 1 second
@15:22:42.24 AFV.select :holdingCoil
@15:22:42.34 delay 1 second
@15:22:43.36 AS.reconfigure ASnorm
@15:22:43.41 AS.seek 0ml
@15:23:34.16 <MAIN> AFV.select :carrierSolution
@15:23:34.27 delay 2 seconds
@15:23:36.29 AS.reconfigure ASconfig
@15:23:36.35 AS.seek 0.5ml
@15:24:05.39 delay 1 second
@15:24:06.41 AFV.select :holdingCoil
@15:24:06.51 ATV.dial :air1
@15:24:08.07 AS.reconfigure ASslow2
@15:24:08.12 AS.seek 0.506ml
@15:24:14.56 ATV.dial :extSrc
@15:24:16.34 AS.seek 0.512ml
@15:24:22.76 delay 5 seconds
@15:24:27.79 ATV.dial :air1
@15:24:29.57 AS.reconfigure ASslow1
@15:24:29.62 AS.seek 0.542ml
@15:24:35.96 ATV.dial :waste
@15:24:36.54 AS.reconfigure ASconfig
@15:24:36.59 AS.seek 0ml
@15:25:08.19 <MFBPCR> Setting up PCR for AOPP1 -- writing /var/log/esp/AOPP121may0615h.pcr
@15:25:08.21 Building PCR Slug from extSrc with MM1 mix, PP1 primer
@15:25:08.23 Priming with carrier
@15:25:08.25 ATV.dial ARV
@15:25:09.81 ARV.dial :carrier
@15:25:10.16 AS.reconfigure ASnorm
@15:25:10.22 AS.seek 0.5ml
@15:25:35.76 AFV.select :carrierSolution
@15:25:35.88 delay 2 seconds
@15:25:37.90 AS.reconfigure ASconfig
@15:25:37.96 AS.seek 1ml
@15:26:06.98 delay 1 second
@15:26:08.01 AFV.select :holdingCoil
@15:26:08.11 delay 1 second
@15:26:09.13 ATV.dial :waste
@15:26:10.90 AS.reconfigure ASnorm
@15:26:10.96 AS.seek 0ml
@15:27:01.64 AFV.select :carrierSolution
@15:27:01.76 delay 2 seconds
@15:27:03.78 AS.reconfigure ASconfig
@15:27:03.84 AS.seek 0.5ml
@15:27:32.85 delay 1 second
@15:27:33.88 AFV.select :holdingCoil
@15:27:33.98 Building Slug
@15:27:33.99 ATV.dial :ARV
@15:27:36.13 delay 2 seconds
@15:27:38.15 ARV.dial :air1
@15:27:38.58 delay 2 seconds
@15:27:40.64 AS.reconfigure ASslow2
@15:27:40.71 AS.seek 0.506ml
@15:27:47.04 delay 2 seconds
@15:27:49.07 ARV.dial :MM1
@15:27:49.70 delay 2 seconds
@15:27:51.73 Adding MM1
@15:27:51.80 AS.seek 0.524ml
@15:28:10.23 delay 25 seconds
@15:28:35.26 ARV.dial :PP1
@15:28:35.66 Adding PP1
@15:28:35.67 delay 2 seconds
@15:28:37.75 AS.seek 0.53ml
@15:28:43.93 delay 10 seconds
@15:28:53.97 ARV.dial :air1
@15:28:54.40 adding ARV air
@15:28:54.41 delay 2 seconds
@15:28:56.49 AS.seek 0.536ml
@15:29:02.93 delay 5 seconds
@15:29:07.96 ARV.dial :carrier
@15:29:08.39 delay 7 seconds
@15:29:15.46 AS.seek 0.594ml
@15:30:14.28 delay 5 seconds
@15:30:19.31 ATV.dial :extSrc
@15:30:19.90 delay 2 seconds
@15:30:21.98 AS.seek 0.6ml
@15:30:28.17 delay 4 seconds
@15:30:32.20 ATV.dial ARV
@15:30:32.76 delay 2 seconds
@15:30:34.79 ARV.dial :carrier
@15:30:34.80 delay 2 seconds
@15:30:36.88 AS.seek 0.65ml
@15:31:27.32 delay 4 seconds
@15:31:31.35 ATV.dial PCR
@15:31:32.15 ASV.dial :waste1
@15:31:32.17 ARV.dial :air1
@15:31:32.60 Positioning Slug by 0.324ml
@15:31:32.69 AS.seek 0.326ml
@15:36:59.09 delay 5 seconds
@15:37:04.12 ATV.dial ARV
@15:37:04.93 ASV.dial :PCRlock
@15:37:05.32 delay 5 seconds
@15:37:10.35 Running AOPP1 Profile60CIQ1 thermocycling
@15:37:10.36 <PCR> PCR.program Profile60CIQ1
@15:37:10.49 Profile60CIQ1 should complete in < 1:56:15
@15:37:10.50 PCR.run 2:30:18.75
@17:16:05.24 PCR.off
@17:16:05.78 <MFBPCR> Running AOPP1 MeltProfile60 thermocycling
@17:16:05.80 <PCR> PCR.program MeltProfile60
@17:16:05.89 MeltProfile60 should complete in < 11:55
@17:16:05.91 PCR.run 19:53.75
@17:23:34.76 PCR.off
@17:23:35.65 <MFBPCR> PCR for AOPP1 completed -- closed /var/log/esp/AOPP121may0615h.pcr
@17:23:35.73 Cleaning PCR
@17:23:35.74 AFV.select :carrierSolution
@17:23:35.86 delay 2 seconds
@17:23:37.88 AS.reconfigure ASconfig
@17:23:37.93 AS.seek 0.5ml
@17:23:48.24 delay 1 second
@17:23:49.27 AFV.select :holdingCoil
@17:23:49.37 ASV.dial :waste1
@17:23:49.76 ATV.dial :air3
@17:23:51.12 AS.reconfigure ASslow1
@17:23:51.17 AS.seek 0.51ml
@17:23:53.43 delay 2 seconds
@17:23:55.46 ATV.dial :bleach
@17:23:56.12 AS.seek 0.81ml
@17:24:56.85 delay 3 seconds
@17:24:59.88 ATV.dial :air3
@17:25:00.48 delay 3 seconds
@17:25:03.55 AS.seek 0.835ml
@17:25:08.93 delay 3 seconds
@17:25:11.96 ATV.dial :bleach
@17:25:12.60 AS.seek 0.82ml
@17:25:16.04 delay 3 seconds
@17:25:19.07 ARV.dial :waste
@17:25:19.73 delay 3 seconds
@17:25:22.76 ATV.dial :ARV
@17:25:23.68 AS.reconfigure ASconfig
@17:25:23.73 AS.seek 0.47ml
@17:25:44.17 AFV.select :carrierSolution
@17:25:44.28 delay 2 seconds
@17:25:46.30 AS.seek 1ml
@17:26:17.04 delay 1 second
@17:26:18.07 AFV.select :holdingCoil
@17:26:18.17 delay 1 second
@17:26:19.19 AS.reconfigure ASfast
@17:26:19.24 AS.seek 0ml
@17:26:46.56 AFV.select :carrierSolution
@17:26:46.68 delay 2 seconds
@17:26:48.70 AS.reconfigure ASconfig
@17:26:48.75 AS.seek 1ml
@17:27:46.51 delay 1 second
@17:27:47.54 AFV.select :holdingCoil
@17:27:47.64 delay 1 second
@17:27:48.66 AS.reconfigure ASfast
@17:27:48.71 AS.seek 0ml
@17:28:16.03 AFV.select :carrierSolution
@17:28:16.15 delay 2 seconds
@17:28:18.17 AS.reconfigure ASconfig
@17:28:18.22 AS.seek 1ml
@17:29:15.96 delay 1 second
@17:29:16.99 AFV.select :holdingCoil
@17:29:17.09 delay 1 second
@17:29:18.11 AS.reconfigure ASfast
@17:29:18.16 AS.seek 0ml
@17:29:45.49 ARV.dial between carrier and air1
@17:29:46.02 ATV.dial :air3
@17:29:47.37 AS.reconfigure ASslow1
@17:29:47.42 AS.seek 0.01ml
@17:29:49.84 delay 2 seconds
@17:29:51.87 ATV.dial :bleach
@17:29:52.51 AS.seek 0.81ml
@17:32:33.70 delay 3 seconds
@17:32:36.73 ATV.dial :air3
@17:32:37.31 delay 3 seconds
@17:32:40.38 AS.seek 0.835ml
@17:32:45.74 delay 3 seconds
@17:32:48.77 ATV.dial :bleach
@17:32:49.41 AS.seek 0.82ml
@17:32:52.84 delay 3 seconds
@17:32:55.87 ATV.dial PCR
@17:32:56.29 AS.reconfigure ASconfig
@17:32:56.34 AS.seek 0ml
@17:33:43.78 PCR.program :bleachProfile
@17:33:43.85 PCR program should complete in < 2:45
@17:33:43.87 PCR.run 10:00
@17:36:40.04 PCR.off
@17:36:40.09 AFV.select :carrierSolution
@17:36:40.20 delay 2 seconds
@17:36:42.22 AS.seek 1ml
@17:37:39.96 delay 1 second
@17:37:40.99 AFV.select :holdingCoil
@17:37:41.09 delay 1 second
@17:37:42.11 AS.reconfigure ASfast
@17:37:42.16 AS.seek 0ml
@17:38:09.50 delay 3 seconds
@17:38:12.53 AFV.select :carrierSolution
@17:38:12.63 delay 2 seconds
@17:38:14.65 AS.reconfigure ASconfig
@17:38:14.72 AS.seek 1ml
@17:39:12.48 delay 1 second
@17:39:13.51 AFV.select :holdingCoil
@17:39:13.61 delay 1 second
@17:39:14.63 AS.reconfigure ASfast
@17:39:14.68 AS.seek 0ml
@17:39:41.99 delay 3 seconds
@17:39:45.02 AFV.select :carrierSolution
@17:39:45.12 delay 2 seconds
@17:39:47.14 AS.reconfigure ASconfig
@17:39:47.20 AS.seek 1ml
@17:40:44.95 delay 1 second
@17:40:45.98 AFV.select :holdingCoil
@17:40:46.08 delay 1 second
@17:40:47.10 AS.reconfigure ASfast
@17:40:47.15 AS.seek 0ml
@17:41:14.49 delay 3 seconds
@17:41:17.52 AFV.select :carrierSolution
@17:41:17.62 delay 2 seconds
@17:41:19.64 AS.reconfigure ASconfig
@17:41:19.71 AS.seek 1ml
@17:42:17.46 delay 1 second
@17:42:18.49 AFV.select :holdingCoil
@17:42:18.59 delay 1 second
@17:42:19.61 AS.reconfigure ASfast
@17:42:19.67 AS.seek 0ml
@17:42:46.99 delay 3 seconds
@17:42:50.02 AFV.select :carrierSolution
@17:42:50.12 delay 2 seconds
@17:42:52.14 AS.reconfigure ASconfig
@17:42:52.20 AS.seek 1ml
@17:43:49.96 delay 1 second
@17:43:50.99 AFV.select :holdingCoil
@17:43:51.09 delay 1 second
@17:43:52.11 AS.reconfigure ASnorm
@17:43:52.16 AS.seek 0ml
@17:44:42.89 <MAIN> AFV.select :carrierSolution
@17:44:43.00 delay 2 seconds
@17:44:45.02 AS.reconfigure ASconfig
@17:44:45.07 AS.seek 0.5ml
@17:45:14.10 delay 1 second
@17:45:15.13 AFV.select :holdingCoil
@17:45:15.23 ATV.dial :air1
@17:45:16.81 AS.reconfigure ASslow2
@17:45:16.86 AS.seek 0.51ml
@17:45:27.07 delay 5 seconds
@17:45:32.10 ATV.dial :extSrc
@17:45:33.88 AS.seek 0.53ml
@17:45:54.34 delay 10 seconds
@17:46:04.37 ATV.dial :air1
@17:46:06.15 AS.reconfigure ASslow1
@17:46:06.20 AS.seek 0.56ml
@17:46:12.54 ATV.dial :waste
@17:46:13.11 AS.reconfigure ASconfig
@17:46:13.17 AS.seek 0ml
@17:46:45.79 <MFBPCR> Setting up PCR for AOPP1 -- writing /var/log/esp/AOPP121may0617h.pcr
@17:46:45.81 Building PCR Slug from extSrc with MM1 mix, PP1 primer
@17:46:45.83 Priming with carrier
@17:46:45.85 ATV.dial ARV
@17:46:47.43 ARV.dial :carrier
@17:46:47.77 AS.reconfigure ASnorm
@17:46:47.83 AS.seek 0.5ml
@17:47:13.40 AFV.select :carrierSolution
@17:47:13.52 delay 2 seconds
@17:47:15.54 AS.reconfigure ASconfig
@17:47:15.60 AS.seek 1ml
@17:47:44.62 delay 1 second
@17:47:45.65 AFV.select :holdingCoil
@17:47:45.75 delay 1 second
@17:47:46.77 ATV.dial :waste
@17:47:48.54 AS.reconfigure ASnorm
@17:47:48.60 AS.seek 0ml
@17:48:39.32 AFV.select :carrierSolution
@17:48:39.44 delay 2 seconds
@17:48:41.46 AS.reconfigure ASconfig
@17:48:41.52 AS.seek 0.5ml
@17:49:10.56 delay 1 second
@17:49:11.59 AFV.select :holdingCoil
@17:49:11.69 Building Slug
@17:49:11.70 ATV.dial :ARV
@17:49:13.44 delay 2 seconds
@17:49:15.46 ARV.dial :air1
@17:49:15.86 delay 2 seconds
@17:49:17.93 AS.reconfigure ASslow2
@17:49:17.99 AS.seek 0.506ml
@17:49:24.67 delay 2 seconds
@17:49:26.70 ARV.dial :MM1
@17:49:27.34 delay 2 seconds
@17:49:29.37 Adding MM1
@17:49:29.44 AS.seek 0.524ml
@17:49:47.99 delay 25 seconds
@17:50:13.02 ARV.dial :PP1
@17:50:13.44 Adding PP1
@17:50:13.81 delay 2 seconds
@17:50:15.90 AS.seek 0.53ml
@17:50:22.32 delay 10 seconds
@17:50:32.36 ARV.dial :air1
@17:50:32.79 adding ARV air
@17:50:32.80 delay 2 seconds
@17:50:34.88 AS.seek 0.536ml
@17:50:41.18 delay 5 seconds
@17:50:46.21 ARV.dial :carrier
@17:50:46.63 delay 7 seconds
@17:50:53.71 AS.seek 0.594ml
@17:51:52.23 delay 5 seconds
@17:51:57.26 ATV.dial :extSrc
@17:51:57.86 delay 2 seconds
@17:51:59.93 AS.seek 0.6ml
@17:52:06.14 delay 4 seconds
@17:52:10.17 ATV.dial ARV
@17:52:10.85 delay 2 seconds
@17:52:12.87 ARV.dial :carrier
@17:52:12.88 delay 2 seconds
@17:52:14.96 AS.seek 0.65ml
@17:53:06.09 delay 4 seconds
@17:53:10.12 ATV.dial PCR
@17:53:10.93 ASV.dial :waste1
@17:53:10.95 ARV.dial :air1
@17:53:11.37 Positioning Slug by 0.324ml
@17:53:11.46 AS.seek 0.326ml
@17:58:37.79 delay 5 seconds
@17:58:42.82 ATV.dial ARV
@17:58:43.64 ASV.dial :PCRlock
@17:58:44.05 delay 5 seconds
@17:58:49.07 Running AOPP1 Profile60CIQ1 thermocycling
@17:58:49.08 <PCR> PCR.program Profile60CIQ1
@17:58:49.20 Profile60CIQ1 should complete in < 1:56:15
@17:58:49.21 PCR.run 2:30:18.75
@19:37:28.59 PCR.off
@19:37:29.14 <MFBPCR> Running AOPP1 MeltProfile60 thermocycling
@19:37:29.15 <PCR> PCR.program MeltProfile60
@19:37:29.26 MeltProfile60 should complete in < 11:55
@19:37:29.27 PCR.run 19:53.75
@19:44:58.00 PCR.off
@19:44:58.92 <MFBPCR> PCR for AOPP1 completed -- closed /var/log/esp/AOPP121may0617h.pcr
@19:44:58.99 Cleaning PCR
@19:44:59.00 AFV.select :carrierSolution
@19:44:59.12 delay 2 seconds
@19:45:01.14 AS.reconfigure ASconfig
@19:45:01.19 AS.seek 0.5ml
@19:45:11.50 delay 1 second
@19:45:12.53 AFV.select :holdingCoil
@19:45:12.63 ASV.dial :waste1
@19:45:13.03 ATV.dial :air3
@19:45:14.38 AS.reconfigure ASslow1
@19:45:14.43 AS.seek 0.51ml
@19:45:16.69 delay 2 seconds
@19:45:18.72 ATV.dial :bleach
@19:45:19.37 AS.seek 0.81ml
@19:46:19.95 delay 3 seconds
@19:46:22.98 ATV.dial :air3
@19:46:23.57 delay 3 seconds
@19:46:26.64 AS.seek 0.835ml
@19:46:31.94 delay 3 seconds
@19:46:34.97 ATV.dial :bleach
@19:46:35.60 AS.seek 0.82ml
@19:46:38.90 delay 3 seconds
@19:46:41.93 ARV.dial :waste
@19:46:42.57 delay 3 seconds
@19:46:45.60 ATV.dial :ARV
@19:46:46.53 AS.reconfigure ASconfig
@19:46:46.58 AS.seek 0.47ml
@19:47:07.00 AFV.select :carrierSolution
@19:47:07.12 delay 2 seconds
@19:47:09.14 AS.seek 1ml
@19:47:39.89 delay 1 second
@19:47:40.92 AFV.select :holdingCoil
@19:47:41.02 delay 1 second
@19:47:42.04 AS.reconfigure ASfast
@19:47:42.09 AS.seek 0ml
@19:48:09.42 AFV.select :carrierSolution
@19:48:09.54 delay 2 seconds
@19:48:11.56 AS.reconfigure ASconfig
@19:48:11.61 AS.seek 1ml
@19:49:09.37 delay 1 second
@19:49:10.40 AFV.select :holdingCoil
@19:49:10.50 delay 1 second
@19:49:11.52 AS.reconfigure ASfast
@19:49:11.57 AS.seek 0ml
@19:49:38.90 AFV.select :carrierSolution
@19:49:39.02 delay 2 seconds
@19:49:41.04 AS.reconfigure ASconfig
@19:49:41.09 AS.seek 1ml
@19:50:38.89 delay 1 second
@19:50:39.92 AFV.select :holdingCoil
@19:50:40.02 delay 1 second
@19:50:41.04 AS.reconfigure ASfast
@19:50:41.09 AS.seek 0ml
@19:51:08.42 ARV.dial between carrier and air1
@19:51:08.96 ATV.dial :air3
@19:51:10.31 AS.reconfigure ASslow1
@19:51:10.37 AS.seek 0.01ml
@19:51:12.67 delay 2 seconds
@19:51:14.70 ATV.dial :bleach
@19:51:15.35 AS.seek 0.81ml
@19:53:56.54 delay 3 seconds
@19:53:59.57 ATV.dial :air3
@19:54:00.17 delay 3 seconds
@19:54:03.24 AS.seek 0.835ml
@19:54:08.54 delay 3 seconds
@19:54:11.57 ATV.dial :bleach
@19:54:12.23 AS.seek 0.82ml
@19:54:15.53 delay 3 seconds
@19:54:18.56 ATV.dial PCR
@19:54:18.98 AS.reconfigure ASconfig
@19:54:19.03 AS.seek 0ml
@19:55:06.45 PCR.program :bleachProfile
@19:55:06.52 PCR program should complete in < 2:45
@19:55:06.53 PCR.run 10:00
@19:58:02.72 PCR.off
@19:58:02.76 AFV.select :carrierSolution
@19:58:02.87 delay 2 seconds
@19:58:04.89 AS.seek 1ml
@19:59:02.65 delay 1 second
@19:59:03.68 AFV.select :holdingCoil
@19:59:03.78 delay 1 second
@19:59:04.80 AS.reconfigure ASfast
@19:59:04.85 AS.seek 0ml
@19:59:32.20 delay 3 seconds
@19:59:35.23 AFV.select :carrierSolution
@19:59:35.33 delay 2 seconds
@19:59:37.35 AS.reconfigure ASconfig
@19:59:37.41 AS.seek 1ml
@20:00:35.17 delay 1 second
@20:00:36.20 AFV.select :holdingCoil
@20:00:36.30 delay 1 second
@20:00:37.32 AS.reconfigure ASfast
@20:00:37.37 AS.seek 0ml
@20:01:04.68 delay 3 seconds
@20:01:07.71 AFV.select :carrierSolution
@20:01:07.81 delay 2 seconds
@20:01:09.83 AS.reconfigure ASconfig
@20:01:09.90 AS.seek 1ml
@20:02:07.65 delay 1 second
@20:02:08.68 AFV.select :holdingCoil
@20:02:08.78 delay 1 second
@20:02:09.80 AS.reconfigure ASfast
@20:02:09.85 AS.seek 0ml
@20:02:37.15 delay 3 seconds
@20:02:40.18 AFV.select :carrierSolution
@20:02:40.28 delay 2 seconds
@20:02:42.30 AS.reconfigure ASconfig
@20:02:42.36 AS.seek 1ml
@20:03:40.11 delay 1 second
@20:03:41.14 AFV.select :holdingCoil
@20:03:41.24 delay 1 second
@20:03:42.26 AS.reconfigure ASfast
@20:03:42.32 AS.seek 0ml
@20:04:09.65 delay 3 seconds
@20:04:12.68 AFV.select :carrierSolution
@20:04:12.78 delay 2 seconds
@20:04:14.80 AS.reconfigure ASconfig
@20:04:14.86 AS.seek 1ml
@20:05:12.61 delay 1 second
@20:05:13.64 AFV.select :holdingCoil
@20:05:13.74 delay 1 second
@20:05:14.76 AS.reconfigure ASnorm
@20:05:14.82 AS.seek 0ml
@20:06:05.52 <MAIN> AFV.select :carrierSolution
@20:06:05.63 delay 2 seconds
@20:06:07.65 AS.reconfigure ASconfig
@20:06:07.70 AS.seek 0.5ml
@20:06:36.72 delay 1 second
@20:06:37.75 AFV.select :holdingCoil
@20:06:37.85 ATV.dial :air1
@20:06:39.42 AS.reconfigure ASslow2
@20:06:39.47 AS.seek 0.506ml
@20:06:45.84 ATV.dial :extSrc
@20:06:47.62 AS.seek 0.512ml
@20:06:53.97 delay 5 seconds
@20:06:59.00 ATV.dial :air1
@20:07:00.81 AS.reconfigure ASslow1
@20:07:00.86 AS.seek 0.542ml
@20:07:07.23 ATV.dial :waste
@20:07:07.83 AS.reconfigure ASconfig
@20:07:07.88 AS.seek 0ml
@20:07:39.47 <MFBPCR> Setting up PCR for AOPP1 -- writing /var/log/esp/AOPP121may0620h.pcr
@20:07:39.49 Building PCR Slug from extSrc with MM1 mix, PP1 primer
@20:07:39.51 Priming with carrier
@20:07:39.53 ATV.dial ARV
@20:07:41.09 ARV.dial :carrier
@20:07:41.80 AS.reconfigure ASnorm
@20:07:41.87 AS.seek 0.5ml
@20:08:07.43 AFV.select :carrierSolution
@20:08:07.56 delay 2 seconds
@20:08:09.58 AS.reconfigure ASconfig
@20:08:09.65 AS.seek 1ml
@20:08:38.65 delay 1 second
@20:08:39.68 AFV.select :holdingCoil
@20:08:39.78 delay 1 second
@20:08:40.80 ATV.dial :waste
@20:08:42.57 AS.reconfigure ASnorm
@20:08:42.63 AS.seek 0ml
@20:09:33.32 AFV.select :carrierSolution
@20:09:33.44 delay 2 seconds
@20:09:35.46 AS.reconfigure ASconfig
@20:09:35.52 AS.seek 0.5ml
@20:10:04.57 delay 1 second
@20:10:05.60 AFV.select :holdingCoil
@20:10:05.70 Building Slug
@20:10:05.71 ATV.dial :ARV
@20:10:07.46 delay 2 seconds
@20:10:09.48 ARV.dial :air1
@20:10:09.88 delay 2 seconds
@20:10:11.95 AS.reconfigure ASslow2
@20:10:12.02 AS.seek 0.506ml
@20:10:19.15 delay 2 seconds
@20:10:21.18 ARV.dial :MM1
@20:10:21.83 delay 2 seconds
@20:10:23.85 Adding MM1
@20:10:23.93 AS.seek 0.524ml
@20:10:42.29 delay 25 seconds
@20:11:07.32 ARV.dial :PP1
@20:11:07.74 Adding PP1
@20:11:07.75 delay 2 seconds
@20:11:09.83 AS.seek 0.53ml
@20:11:16.39 delay 10 seconds
@20:11:26.43 ARV.dial :air1
@20:11:26.85 adding ARV air
@20:11:26.86 delay 2 seconds
@20:11:28.94 AS.seek 0.536ml
@20:11:35.29 delay 5 seconds
@20:11:40.32 ARV.dial :carrier
@20:11:40.75 delay 7 seconds
@20:11:47.82 AS.seek 0.594ml
@20:12:46.61 delay 5 seconds
@20:12:51.64 ATV.dial :extSrc
@20:12:52.23 delay 2 seconds
@20:12:54.31 AS.seek 0.6ml
@20:13:00.65 delay 4 seconds
@20:13:04.68 ATV.dial ARV
@20:13:05.36 delay 2 seconds
@20:13:07.39 ARV.dial :carrier
@20:13:07.40 delay 2 seconds
@20:13:09.48 AS.seek 0.65ml
@20:14:00.54 delay 4 seconds
@20:14:04.57 ATV.dial PCR
@20:14:05.37 ASV.dial :waste1
@20:14:05.39 ARV.dial :air1
@20:14:05.81 Positioning Slug by 0.324ml
@20:14:05.89 AS.seek 0.326ml
@20:19:32.17 delay 5 seconds
@20:19:37.20 ATV.dial ARV
@20:19:38.01 ASV.dial :PCRlock
@20:19:38.43 delay 5 seconds
@20:19:43.45 Running AOPP1 Profile60CIQ1 thermocycling
@20:19:43.46 <PCR> PCR.program Profile60CIQ1
@20:19:43.58 Profile60CIQ1 should complete in < 1:56:15
@20:19:43.59 PCR.run 2:30:18.75
@21:58:15.81 PCR.off
@21:58:16.36 <MFBPCR> Running AOPP1 MeltProfile60 thermocycling
@21:58:16.38 <PCR> PCR.program MeltProfile60
@21:58:16.48 MeltProfile60 should complete in < 11:55
@21:58:16.49 PCR.run 19:53.75
@22:05:48.29 PCR.off
@22:05:49.21 <MFBPCR> PCR for AOPP1 completed -- closed /var/log/esp/AOPP121may0620h.pcr
@22:05:49.28 Cleaning PCR
@22:05:49.29 AFV.select :carrierSolution
@22:05:49.40 delay 2 seconds
@22:05:51.42 AS.reconfigure ASconfig
@22:05:51.47 AS.seek 0.5ml
@22:06:01.80 delay 1 second
@22:06:02.83 AFV.select :holdingCoil
@22:06:02.93 ASV.dial :waste1
@22:06:03.33 ATV.dial :air3
@22:06:04.68 AS.reconfigure ASslow1
@22:06:04.74 AS.seek 0.51ml
@22:06:07.04 delay 2 seconds
@22:06:09.07 ATV.dial :bleach
@22:06:09.71 AS.seek 0.81ml
@22:07:10.30 delay 3 seconds
@22:07:13.33 ATV.dial :air3
@22:07:13.91 delay 3 seconds
@22:07:16.98 AS.seek 0.835ml
@22:07:22.27 delay 3 seconds
@22:07:25.30 ATV.dial :bleach
@22:07:25.93 AS.seek 0.82ml
@22:07:29.36 delay 3 seconds
@22:07:32.39 ARV.dial :waste
@22:07:33.01 delay 3 seconds
@22:07:36.04 ATV.dial :ARV
@22:07:36.97 AS.reconfigure ASconfig
@22:07:37.02 AS.seek 0.47ml
@22:07:57.45 AFV.select :carrierSolution
@22:07:57.57 delay 2 seconds
@22:07:59.59 AS.seek 1ml
@22:08:30.33 delay 1 second
@22:08:31.36 AFV.select :holdingCoil
@22:08:31.46 delay 1 second
@22:08:32.48 AS.reconfigure ASfast
@22:08:32.53 AS.seek 0ml
@22:08:59.87 AFV.select :carrierSolution
@22:08:59.99 delay 2 seconds
@22:09:02.01 AS.reconfigure ASconfig
@22:09:02.06 AS.seek 1ml
@22:09:59.84 delay 1 second
@22:10:00.87 AFV.select :holdingCoil
@22:10:00.97 delay 1 second
@22:10:01.99 AS.reconfigure ASfast
@22:10:02.04 AS.seek 0ml
@22:10:29.36 AFV.select :carrierSolution
@22:10:29.48 delay 2 seconds
@22:10:31.50 AS.reconfigure ASconfig
@22:10:31.55 AS.seek 1ml
@22:11:29.31 delay 1 second
@22:11:30.34 AFV.select :holdingCoil
@22:11:30.44 delay 1 second
@22:11:31.46 AS.reconfigure ASfast
@22:11:31.51 AS.seek 0ml
@22:11:59.23 ARV.dial between carrier and air1
@22:11:59.74 ATV.dial :air3
@22:12:01.09 AS.reconfigure ASslow1
@22:12:01.15 AS.seek 0.01ml
@22:12:03.47 delay 2 seconds
@22:12:05.50 ATV.dial :bleach
@22:12:06.15 AS.seek 0.81ml
@22:14:47.33 delay 3 seconds
@22:14:50.36 ATV.dial :air3
@22:14:50.94 delay 3 seconds
@22:14:54.02 AS.seek 0.835ml
@22:14:59.37 delay 3 seconds
@22:15:02.40 ATV.dial :bleach
@22:15:03.04 AS.seek 0.82ml
@22:15:06.33 delay 3 seconds
@22:15:09.36 ATV.dial PCR
@22:15:09.77 AS.reconfigure ASconfig
@22:15:09.82 AS.seek 0ml
@22:15:57.23 PCR.program :bleachProfile
@22:15:57.30 PCR program should complete in < 2:45
@22:15:57.31 PCR.run 10:00
@22:18:53.10 PCR.off
@22:18:53.15 AFV.select :carrierSolution
@22:18:53.26 delay 2 seconds
@22:18:55.28 AS.seek 1ml
@22:19:53.03 delay 1 second
@22:19:54.06 AFV.select :holdingCoil
@22:19:54.16 delay 1 second
@22:19:55.18 AS.reconfigure ASfast
@22:19:55.23 AS.seek 0ml
@22:20:22.56 delay 3 seconds
@22:20:25.59 AFV.select :carrierSolution
@22:20:25.69 delay 2 seconds
@22:20:27.71 AS.reconfigure ASconfig
@22:20:27.77 AS.seek 1ml
@22:21:25.51 delay 1 second
@22:21:26.54 AFV.select :holdingCoil
@22:21:26.64 delay 1 second
@22:21:27.66 AS.reconfigure ASfast
@22:21:27.72 AS.seek 0ml
@22:21:55.05 delay 3 seconds
@22:21:58.08 AFV.select :carrierSolution
@22:21:58.18 delay 2 seconds
@22:22:00.20 AS.reconfigure ASconfig
@22:22:00.25 AS.seek 1ml
@22:22:58.01 delay 1 second
@22:22:59.04 AFV.select :holdingCoil
@22:22:59.14 delay 1 second
@22:23:00.16 AS.reconfigure ASfast
@22:23:00.21 AS.seek 0ml
@22:23:27.53 delay 3 seconds
@22:23:30.56 AFV.select :carrierSolution
@22:23:30.66 delay 2 seconds
@22:23:32.69 AS.reconfigure ASconfig
@22:23:32.75 AS.seek 1ml
@22:24:30.50 delay 1 second
@22:24:31.53 AFV.select :holdingCoil
@22:24:31.63 delay 1 second
@22:24:32.65 AS.reconfigure ASfast
@22:24:32.70 AS.seek 0ml
@22:25:00.01 delay 3 seconds
@22:25:03.04 AFV.select :carrierSolution
@22:25:03.14 delay 2 seconds
@22:25:05.16 AS.reconfigure ASconfig
@22:25:05.23 AS.seek 1ml
@22:26:03.37 delay 1 second
@22:26:04.40 AFV.select :holdingCoil
@22:26:04.50 delay 1 second
@22:26:05.52 AS.reconfigure ASnorm
@22:26:05.57 AS.seek 0ml
@22:26:56.24 <MAIN> AFV.select :carrierSolution
@22:26:56.36 delay 2 seconds
@22:26:58.38 AS.reconfigure ASconfig
@22:26:58.44 AS.seek 0.5ml
@22:27:27.50 delay 1 second
@22:27:28.53 AFV.select :holdingCoil
@22:27:28.63 ATV.dial :air1
@22:27:30.20 AS.reconfigure ASslow1
@22:27:30.25 AS.seek 0.52ml
@22:27:34.58 delay 5 seconds
@22:27:39.61 ATV.dial :extSrc
@22:27:41.40 AS.reconfigure ASslow2
@22:27:41.45 AS.seek 0.545ml
@22:28:06.76 delay 10 seconds
@22:28:16.79 ATV.dial :air1
@22:28:18.59 AS.reconfigure ASslow1
@22:28:18.64 AS.seek 0.575ml
@22:28:24.92 ATV.dial :waste
@22:28:25.52 AS.reconfigure ASconfig
@22:28:25.57 AS.seek 0ml
@22:28:59.08 <MFBPCR> Setting up PCR for AOPP1 -- writing /var/log/esp/AOPP121may0622h.pcr
@22:28:59.11 Building PCR Slug from extSrc with MM1 mix, PP1 primer
@22:28:59.13 Priming with carrier
@22:28:59.14 ATV.dial ARV
@22:29:00.72 ARV.dial :carrier
@22:29:01.06 AS.reconfigure ASnorm
@22:29:01.13 AS.seek 0.5ml
@22:29:26.64 AFV.select :carrierSolution
@22:29:26.77 delay 2 seconds
@22:29:28.79 AS.reconfigure ASconfig
@22:29:28.85 AS.seek 1ml
@22:29:57.86 delay 1 second
@22:29:58.89 AFV.select :holdingCoil
@22:29:58.99 delay 1 second
@22:30:00.01 ATV.dial :waste
@22:30:01.77 AS.reconfigure ASnorm
@22:30:01.83 AS.seek 0ml
@22:30:52.53 AFV.select :carrierSolution
@22:30:52.65 delay 2 seconds
@22:30:54.67 AS.reconfigure ASconfig
@22:30:54.73 AS.seek 0.5ml
@22:31:23.75 delay 1 second
@22:31:24.78 AFV.select :holdingCoil
@22:31:24.88 Building Slug
@22:31:24.89 ATV.dial :ARV
@22:31:26.63 delay 2 seconds
@22:31:28.65 ARV.dial :air1
@22:31:29.07 delay 2 seconds
@22:31:31.14 AS.reconfigure ASslow2
@22:31:31.20 AS.seek 0.506ml
@22:31:38.00 delay 2 seconds
@22:31:40.03 ARV.dial :MM1
@22:31:40.66 delay 2 seconds
@22:31:42.69 Adding MM1
@22:31:42.77 AS.seek 0.524ml
@22:32:00.98 delay 25 seconds
@22:32:26.01 ARV.dial :PP1
@22:32:26.43 Adding PP1
@22:32:26.44 delay 2 seconds
@22:32:28.51 AS.seek 0.53ml
@22:32:35.01 delay 10 seconds
@22:32:45.05 ARV.dial :air1
@22:32:45.46 adding ARV air
@22:32:45.47 delay 2 seconds
@22:32:47.55 AS.seek 0.536ml
@22:32:54.11 delay 5 seconds
@22:32:59.14 ARV.dial :carrier
@22:32:59.56 delay 7 seconds
@22:33:06.63 AS.seek 0.594ml
@22:34:05.51 delay 5 seconds
@22:34:10.54 ATV.dial :extSrc
@22:34:11.12 delay 2 seconds
@22:34:13.20 AS.seek 0.6ml
@22:34:19.72 delay 4 seconds
@22:34:23.75 ATV.dial ARV
@22:34:24.33 delay 2 seconds
@22:34:26.36 ARV.dial :carrier
@22:34:26.37 delay 2 seconds
@22:34:28.44 AS.seek 0.65ml
@22:35:19.20 delay 4 seconds
@22:35:23.23 ATV.dial PCR
@22:35:24.05 ASV.dial :waste1
@22:35:24.07 ARV.dial :air1
@22:35:24.48 Positioning Slug by 0.324ml
@22:35:24.56 AS.seek 0.326ml
@22:40:51.01 delay 5 seconds
@22:40:56.04 ATV.dial ARV
@22:40:56.84 ASV.dial :PCRlock
@22:40:57.26 delay 5 seconds
@22:41:02.28 Running AOPP1 Profile60CIQ1 thermocycling
@22:41:02.30 <PCR> PCR.program Profile60CIQ1
@22:41:02.41 Profile60CIQ1 should complete in < 1:56:15
@22:41:02.42 PCR.run 2:30:18.75
@00:20:01.30CEST07-May-21 PCR.off
@00:20:01.87 <MFBPCR> Running AOPP1 MeltProfile60 thermocycling
@00:20:01.88 <PCR> PCR.program MeltProfile60
@00:20:01.99 MeltProfile60 should complete in < 11:55
@00:20:02.00 PCR.run 19:53.75
@00:27:39.43 PCR.off
@00:27:40.34 <MFBPCR> PCR for AOPP1 completed -- closed /var/log/esp/AOPP121may0622h.pcr
@00:27:40.41 Cleaning PCR
@00:27:40.42 AFV.select :carrierSolution
@00:27:40.54 delay 2 seconds
@00:27:42.56 AS.reconfigure ASconfig
@00:27:42.62 AS.seek 0.5ml
@00:27:52.91 delay 1 second
@00:27:53.94 AFV.select :holdingCoil
@00:27:54.04 ASV.dial :waste1
@00:27:54.44 ATV.dial :air3
@00:27:56.15 AS.reconfigure ASslow1
@00:27:56.21 AS.seek 0.51ml
@00:27:58.45 delay 2 seconds
@00:28:00.48 ATV.dial :bleach
@00:28:01.14 AS.seek 0.81ml
@00:29:01.81 delay 3 seconds
@00:29:04.84 ATV.dial :air3
@00:29:05.42 delay 3 seconds
@00:29:08.49 AS.seek 0.835ml
@00:29:13.85 delay 3 seconds
@00:29:16.87 ATV.dial :bleach
@00:29:17.51 AS.seek 0.82ml
@00:29:20.81 delay 3 seconds
@00:29:23.84 ARV.dial :waste
@00:29:24.48 delay 3 seconds
@00:29:27.51 ATV.dial :ARV
@00:29:28.44 AS.reconfigure ASconfig
@00:29:28.49 AS.seek 0.47ml
@00:29:48.89 AFV.select :carrierSolution
@00:29:49.01 delay 2 seconds
@00:29:51.03 AS.seek 1ml
@00:30:21.78 delay 1 second
@00:30:22.81 AFV.select :holdingCoil
@00:30:22.91 delay 1 second
@00:30:23.93 AS.reconfigure ASfast
@00:30:23.98 AS.seek 0ml
@00:30:51.31 AFV.select :carrierSolution
@00:30:51.43 delay 2 seconds
@00:30:53.45 AS.reconfigure ASconfig
@00:30:53.50 AS.seek 1ml
@00:31:51.25 delay 1 second
@00:31:52.28 AFV.select :holdingCoil
@00:31:52.38 delay 1 second
@00:31:53.40 AS.reconfigure ASfast
@00:31:53.45 AS.seek 0ml
@00:32:20.78 AFV.select :carrierSolution
@00:32:20.89 delay 2 seconds
@00:32:22.91 AS.reconfigure ASconfig
@00:32:22.96 AS.seek 1ml
@00:33:20.72 delay 1 second
@00:33:21.75 AFV.select :holdingCoil
@00:33:21.85 delay 1 second
@00:33:22.87 AS.reconfigure ASfast
@00:33:22.92 AS.seek 0ml
@00:33:50.25 ARV.dial between carrier and air1
@00:33:50.79 ATV.dial :air3
@00:33:52.14 AS.reconfigure ASslow1
@00:33:52.19 AS.seek 0.01ml
@00:33:54.59 delay 2 seconds
@00:33:56.62 ATV.dial :bleach
@00:33:57.26 AS.seek 0.81ml
@00:36:38.41 delay 3 seconds
@00:36:41.44 ATV.dial :air3
@00:36:42.03 delay 3 seconds
@00:36:45.11 AS.seek 0.835ml
@00:36:50.47 delay 3 seconds
@00:36:53.50 ATV.dial :bleach
@00:36:54.14 AS.seek 0.82ml
@00:36:57.53 delay 3 seconds
@00:37:00.56 ATV.dial PCR
@00:37:00.99 AS.reconfigure ASconfig
@00:37:01.05 AS.seek 0ml
@00:37:48.47 PCR.program :bleachProfile
@00:37:48.54 PCR program should complete in < 2:45
@00:37:48.55 PCR.run 10:00
@00:40:44.74 PCR.off
@00:40:44.77 AFV.select :carrierSolution
@00:40:44.89 delay 2 seconds
@00:40:46.91 AS.seek 1ml
@00:41:44.69 delay 1 second
@00:41:45.72 AFV.select :holdingCoil
@00:41:45.82 delay 1 second
@00:41:46.84 AS.reconfigure ASfast
@00:41:46.89 AS.seek 0ml
@00:42:14.25 delay 3 seconds
@00:42:17.28 AFV.select :carrierSolution
@00:42:17.38 delay 2 seconds
@00:42:19.40 AS.reconfigure ASconfig
@00:42:19.45 AS.seek 1ml
@00:43:17.20 delay 1 second
@00:43:18.23 AFV.select :holdingCoil
@00:43:18.33 delay 1 second
@00:43:19.35 AS.reconfigure ASfast
@00:43:19.41 AS.seek 0ml
@00:43:46.75 delay 3 seconds
@00:43:49.78 AFV.select :carrierSolution
@00:43:49.88 delay 2 seconds
@00:43:51.90 AS.reconfigure ASconfig
@00:43:51.96 AS.seek 1ml
@00:44:49.72 delay 1 second
@00:44:50.75 AFV.select :holdingCoil
@00:44:50.85 delay 1 second
@00:44:51.87 AS.reconfigure ASfast
@00:44:51.92 AS.seek 0ml
@00:45:19.25 delay 3 seconds
@00:45:22.28 AFV.select :carrierSolution
@00:45:22.38 delay 2 seconds
@00:45:24.40 AS.reconfigure ASconfig
@00:45:24.45 AS.seek 1ml
@00:46:22.19 delay 1 second
@00:46:23.22 AFV.select :holdingCoil
@00:46:23.32 delay 1 second
@00:46:24.34 AS.reconfigure ASfast
@00:46:24.40 AS.seek 0ml
@00:46:51.73 delay 3 seconds
@00:46:54.76 AFV.select :carrierSolution
@00:46:54.86 delay 2 seconds
@00:46:56.88 AS.reconfigure ASconfig
@00:46:56.94 AS.seek 1ml
@00:47:54.72 delay 1 second
@00:47:55.75 AFV.select :holdingCoil
@00:47:55.85 delay 1 second
@00:47:56.87 AS.reconfigure ASnorm
@00:47:56.92 AS.seek 0ml
@00:48:47.61 <MAIN> AFV.select :carrierSolution
@00:48:47.72 delay 2 seconds
@00:48:49.74 AS.reconfigure ASconfig
@00:48:49.80 AS.seek 0.5ml
@00:49:18.86 delay 1 second
@00:49:19.89 AFV.select :holdingCoil
@00:49:19.99 ATV.dial :air1
@00:49:21.58 AS.reconfigure ASslow2
@00:49:21.63 AS.seek 0.506ml
@00:49:28.25 ATV.dial :extSrc
@00:49:30.04 AS.seek 0.512ml
@00:49:36.36 delay 5 seconds
@00:49:41.39 ATV.dial :air1
@00:49:43.19 AS.reconfigure ASslow1
@00:49:43.24 AS.seek 0.542ml
@00:49:49.59 ATV.dial :waste
@00:49:50.18 AS.reconfigure ASconfig
@00:49:50.23 AS.seek 0ml
@00:50:21.84 <MFBPCR> Setting up PCR for AOPP1 -- writing /var/log/esp/AOPP121may0700h.pcr
@00:50:21.86 Building PCR Slug from extSrc with MM1 mix, PP1 primer
@00:50:21.89 Priming with carrier
@00:50:21.90 ATV.dial ARV
@00:50:23.47 ARV.dial :carrier
@00:50:23.83 AS.reconfigure ASnorm
@00:50:23.89 AS.seek 0.5ml
@00:50:49.42 AFV.select :carrierSolution
@00:50:49.54 delay 2 seconds
@00:50:51.56 AS.reconfigure ASconfig
@00:50:51.62 AS.seek 1ml
@00:51:20.63 delay 1 second
@00:51:21.66 AFV.select :holdingCoil
@00:51:21.76 delay 1 second
@00:51:22.78 ATV.dial :waste
@00:51:24.56 AS.reconfigure ASnorm
@00:51:24.62 AS.seek 0ml
@00:52:15.31 AFV.select :carrierSolution
@00:52:15.43 delay 2 seconds
@00:52:17.45 AS.reconfigure ASconfig
@00:52:17.51 AS.seek 0.5ml
@00:52:46.53 delay 1 second
@00:52:47.56 AFV.select :holdingCoil
@00:52:47.66 Building Slug
@00:52:47.67 ATV.dial :ARV
@00:52:49.43 delay 2 seconds
@00:52:51.45 ARV.dial :air1
@00:52:51.85 delay 2 seconds
@00:52:53.92 AS.reconfigure ASslow2
@00:52:53.98 AS.seek 0.506ml
@00:53:00.28 delay 2 seconds
@00:53:02.31 ARV.dial :MM1
@00:53:02.95 delay 2 seconds
@00:53:04.98 Adding MM1
@00:53:05.05 AS.seek 0.524ml
@00:53:23.25 delay 25 seconds
@00:53:48.28 ARV.dial :PP1
@00:53:48.70 Adding PP1
@00:53:48.71 delay 2 seconds
@00:53:50.78 AS.seek 0.53ml
@00:53:57.42 delay 10 seconds
@00:54:07.46 ARV.dial :air1
@00:54:07.89 adding ARV air
@00:54:07.90 delay 2 seconds
@00:54:09.97 AS.seek 0.536ml
@00:54:16.59 delay 5 seconds
@00:54:21.62 ARV.dial :carrier
@00:54:22.03 delay 7 seconds
@00:54:29.10 AS.seek 0.594ml
@00:55:27.53 delay 5 seconds
@00:55:32.56 ATV.dial :extSrc
@00:55:33.55 delay 2 seconds
@00:55:35.63 AS.seek 0.6ml
@00:55:42.33 delay 4 seconds
@00:55:46.36 ATV.dial ARV
@00:55:46.93 delay 2 seconds
@00:55:48.95 ARV.dial :carrier
@00:55:48.96 delay 2 seconds
@00:55:51.04 AS.seek 0.65ml
@00:56:41.56 delay 4 seconds
@00:56:45.59 ATV.dial PCR
@00:56:46.39 ASV.dial :waste1
@00:56:46.41 ARV.dial :air1
@00:56:46.83 Positioning Slug by 0.324ml
@00:56:46.92 AS.seek 0.326ml
@01:02:12.91 delay 5 seconds
@01:02:17.94 ATV.dial ARV
@01:02:18.75 ASV.dial :PCRlock
@01:02:19.17 delay 5 seconds
@01:02:24.19 Running AOPP1 Profile60CIQ1 thermocycling
@01:02:24.20 <PCR> PCR.program Profile60CIQ1
@01:02:24.32 Profile60CIQ1 should complete in < 1:56:15
@01:02:24.33 PCR.run 2:30:18.75
@02:41:20.35 PCR.off
@02:41:20.91 <MFBPCR> Running AOPP1 MeltProfile60 thermocycling
@02:41:20.92 <PCR> PCR.program MeltProfile60
@02:41:21.38 MeltProfile60 should complete in < 11:55
@02:41:21.39 PCR.run 19:53.75
@02:48:56.25 PCR.off
@02:48:57.17 <MFBPCR> PCR for AOPP1 completed -- closed /var/log/esp/AOPP121may0700h.pcr
@02:48:57.24 Cleaning PCR
@02:48:57.25 AFV.select :carrierSolution
@02:48:57.38 delay 2 seconds
@02:48:59.40 AS.reconfigure ASconfig
@02:48:59.45 AS.seek 0.5ml
@02:49:09.74 delay 1 second
@02:49:10.77 AFV.select :holdingCoil
@02:49:10.87 ASV.dial :waste1
@02:49:11.27 ATV.dial :air3
@02:49:12.62 AS.reconfigure ASslow1
@02:49:12.68 AS.seek 0.51ml
@02:49:14.95 delay 2 seconds
@02:49:16.98 ATV.dial :bleach
@02:49:17.62 AS.seek 0.81ml
@02:50:18.20 delay 3 seconds
@02:50:21.23 ATV.dial :air3
@02:50:21.81 delay 3 seconds
@02:50:24.88 AS.seek 0.835ml
@02:50:30.26 delay 3 seconds
@02:50:33.29 ATV.dial :bleach
@02:50:33.94 AS.seek 0.82ml
@02:50:37.25 delay 3 seconds
@02:50:40.28 ARV.dial :waste
@02:50:40.91 delay 3 seconds
@02:50:43.94 ATV.dial :ARV
@02:50:44.86 AS.reconfigure ASconfig
@02:50:44.91 AS.seek 0.47ml
@02:51:05.31 AFV.select :carrierSolution
@02:51:05.42 delay 2 seconds
@02:51:07.44 AS.seek 1ml
@02:51:38.17 delay 1 second
@02:51:39.20 AFV.select :holdingCoil
@02:51:39.30 delay 1 second
@02:51:40.32 AS.reconfigure ASfast
@02:51:40.37 AS.seek 0ml
@02:52:07.69 AFV.select :carrierSolution
@02:52:07.81 delay 2 seconds
@02:52:09.83 AS.reconfigure ASconfig
@02:52:09.88 AS.seek 1ml
@02:53:07.64 delay 1 second
@02:53:08.67 AFV.select :holdingCoil
@02:53:08.77 delay 1 second
@02:53:09.79 AS.reconfigure ASfast
@02:53:09.84 AS.seek 0ml
@02:53:37.17 AFV.select :carrierSolution
@02:53:37.29 delay 2 seconds
@02:53:39.31 AS.reconfigure ASconfig
@02:53:39.36 AS.seek 1ml
@02:54:37.10 delay 1 second
@02:54:38.13 AFV.select :holdingCoil
@02:54:38.23 delay 1 second
@02:54:39.25 AS.reconfigure ASfast
@02:54:39.30 AS.seek 0ml
@02:55:06.63 ARV.dial between carrier and air1
@02:55:07.17 ATV.dial :air3
@02:55:08.51 AS.reconfigure ASslow1
@02:55:08.57 AS.seek 0.01ml
@02:55:10.89 delay 2 seconds
@02:55:12.92 ATV.dial :bleach
@02:55:13.56 AS.seek 0.81ml
@02:57:54.67 delay 3 seconds
@02:57:57.70 ATV.dial :air3
@02:57:58.28 delay 3 seconds
@02:58:01.35 AS.seek 0.835ml
@02:58:06.75 delay 3 seconds
@02:58:09.78 ATV.dial :bleach
@02:58:10.42 AS.seek 0.82ml
@02:58:13.72 delay 3 seconds
@02:58:16.75 ATV.dial PCR
@02:58:17.17 AS.reconfigure ASconfig
@02:58:17.22 AS.seek 0ml
@02:59:04.63 PCR.program :bleachProfile
@02:59:04.70 PCR program should complete in < 2:45
@02:59:04.72 PCR.run 10:00
@03:02:00.89 PCR.off
@03:02:00.93 AFV.select :carrierSolution
@03:02:01.04 delay 2 seconds
@03:02:03.06 AS.seek 1ml
@03:03:00.81 delay 1 second
@03:03:01.84 AFV.select :holdingCoil
@03:03:01.94 delay 1 second
@03:03:02.96 AS.reconfigure ASfast
@03:03:03.02 AS.seek 0ml
@03:03:30.35 delay 3 seconds
@03:03:33.38 AFV.select :carrierSolution
@03:03:33.48 delay 2 seconds
@03:03:35.50 AS.reconfigure ASconfig
@03:03:35.55 AS.seek 1ml
@03:04:33.28 delay 1 second
@03:04:34.31 AFV.select :holdingCoil
@03:04:34.41 delay 1 second
@03:04:35.43 AS.reconfigure ASfast
@03:04:35.49 AS.seek 0ml
@03:05:02.83 delay 3 seconds
@03:05:05.86 AFV.select :carrierSolution
@03:05:05.96 delay 2 seconds
@03:05:07.98 AS.reconfigure ASconfig
@03:05:08.04 AS.seek 1ml
@03:06:05.78 delay 1 second
@03:06:06.81 AFV.select :holdingCoil
@03:06:06.91 delay 1 second
@03:06:07.93 AS.reconfigure ASfast
@03:06:07.98 AS.seek 0ml
@03:06:35.28 delay 3 seconds
@03:06:38.31 AFV.select :carrierSolution
@03:06:38.41 delay 2 seconds
@03:06:40.43 AS.reconfigure ASconfig
@03:06:40.48 AS.seek 1ml
@03:07:38.24 delay 1 second
@03:07:39.27 AFV.select :holdingCoil
@03:07:39.37 delay 1 second
@03:07:40.39 AS.reconfigure ASfast
@03:07:40.44 AS.seek 0ml
@03:08:07.77 delay 3 seconds
@03:08:10.80 AFV.select :carrierSolution
@03:08:10.90 delay 2 seconds
@03:08:12.92 AS.reconfigure ASconfig
@03:08:12.98 AS.seek 1ml
@03:09:10.72 delay 1 second
@03:09:11.75 AFV.select :holdingCoil
@03:09:11.85 delay 1 second
@03:09:12.87 AS.reconfigure ASnorm
@03:09:12.93 AS.seek 0ml
@03:10:03.63 <MAIN> AFV.select :carrierSolution
@03:10:03.74 delay 2 seconds
@03:10:05.76 AS.reconfigure ASconfig
@03:10:05.81 AS.seek 0.5ml
@03:10:34.83 delay 1 second
@03:10:35.86 AFV.select :holdingCoil
@03:10:35.96 ATV.dial :air1
@03:10:37.53 AS.reconfigure ASslow1
@03:10:37.58 AS.seek 0.52ml
@03:10:41.89 delay 5 seconds
@03:10:46.92 ATV.dial :extSrc
@03:10:48.70 AS.reconfigure ASslow2
@03:10:48.75 AS.seek 0.538ml
@03:11:07.35 delay 10 seconds
@03:11:17.38 ATV.dial :air1
@03:11:19.16 AS.reconfigure ASslow1
@03:11:19.21 AS.seek 0.568ml
@03:11:25.52 ATV.dial :waste
@03:11:26.11 AS.reconfigure ASconfig
@03:11:26.16 AS.seek 0ml
@03:11:59.26 <MFBPCR> Setting up PCR for AOPP1 -- writing /var/log/esp/AOPP121may0703h.pcr
@03:11:59.29 Building PCR Slug from extSrc with MM1 mix, PP1 primer
@03:11:59.31 Priming with carrier
@03:11:59.33 ATV.dial ARV
@03:12:00.91 ARV.dial :carrier
@03:12:01.28 AS.reconfigure ASnorm
@03:12:01.34 AS.seek 0.5ml
@03:12:26.97 AFV.select :carrierSolution
@03:12:27.09 delay 2 seconds
@03:12:29.11 AS.reconfigure ASconfig
@03:12:29.17 AS.seek 1ml
@03:12:58.22 delay 1 second
@03:12:59.25 AFV.select :holdingCoil
@03:12:59.73 delay 1 second
@03:13:00.75 ATV.dial :waste
@03:13:02.53 AS.reconfigure ASnorm
@03:13:02.59 AS.seek 0ml
@03:13:53.28 AFV.select :carrierSolution
@03:13:53.41 delay 2 seconds
@03:13:55.43 AS.reconfigure ASconfig
@03:13:55.49 AS.seek 0.5ml
@03:14:24.50 delay 1 second
@03:14:25.53 AFV.select :holdingCoil
@03:14:25.63 Building Slug
@03:14:25.64 ATV.dial :ARV
@03:14:27.40 delay 2 seconds
@03:14:29.42 ARV.dial :air1
@03:14:29.82 delay 2 seconds
@03:14:31.89 AS.reconfigure ASslow2
@03:14:31.95 AS.seek 0.506ml
@03:14:38.64 delay 2 seconds
@03:14:40.67 ARV.dial :MM1
@03:14:41.32 delay 2 seconds
@03:14:43.34 Adding MM1
@03:14:43.41 AS.seek 0.524ml
@03:15:01.97 delay 25 seconds
@03:15:27.00 ARV.dial :PP1
@03:15:27.42 Adding PP1
@03:15:27.43 delay 2 seconds
@03:15:29.51 AS.seek 0.53ml
@03:15:35.93 delay 10 seconds
@03:15:45.96 ARV.dial :air1
@03:15:46.39 adding ARV air
@03:15:46.40 delay 2 seconds
@03:15:48.47 AS.seek 0.536ml
@03:15:55.05 delay 5 seconds
@03:16:00.08 ARV.dial :carrier
@03:16:00.51 delay 7 seconds
@03:16:07.59 AS.seek 0.594ml
@03:17:06.49 delay 5 seconds
@03:17:11.52 ATV.dial :extSrc
@03:17:12.11 delay 2 seconds
@03:17:14.19 AS.seek 0.6ml
@03:17:20.97 delay 4 seconds
@03:17:25.00 ATV.dial ARV
@03:17:25.56 delay 2 seconds
@03:17:27.59 ARV.dial :carrier
@03:17:27.60 delay 2 seconds
@03:17:29.68 AS.seek 0.65ml
@03:18:20.47 delay 4 seconds
@03:18:24.50 ATV.dial PCR
@03:18:25.30 ASV.dial :waste1
@03:18:25.32 ARV.dial :air1
@03:18:25.74 Positioning Slug by 0.324ml
@03:18:25.82 AS.seek 0.326ml
@03:23:52.08 delay 5 seconds
@03:23:57.11 ATV.dial ARV
@03:23:57.92 ASV.dial :PCRlock
@03:23:58.32 delay 5 seconds
@03:24:03.34 Running AOPP1 Profile60CIQ1 thermocycling
@03:24:03.35 <PCR> PCR.program Profile60CIQ1
@03:24:03.47 Profile60CIQ1 should complete in < 1:56:15
@03:24:03.48 PCR.run 2:30:18.75
@05:02:58.79 PCR.off
@05:02:59.34 <MFBPCR> Running AOPP1 MeltProfile60 thermocycling
@05:02:59.36 <PCR> PCR.program MeltProfile60
@05:02:59.47 MeltProfile60 should complete in < 11:55
@05:02:59.48 PCR.run 19:53.75
@05:10:31.43 PCR.off
@05:10:32.34 <MFBPCR> PCR for AOPP1 completed -- closed /var/log/esp/AOPP121may0703h.pcr
@05:10:32.42 Cleaning PCR
@05:10:32.43 AFV.select :carrierSolution
@05:10:32.55 delay 2 seconds
@05:10:34.57 AS.reconfigure ASconfig
@05:10:34.62 AS.seek 0.5ml
@05:10:44.92 delay 1 second
@05:10:45.95 AFV.select :holdingCoil
@05:10:46.05 ASV.dial :waste1
@05:10:46.44 ATV.dial :air3
@05:10:47.80 AS.reconfigure ASslow1
@05:10:47.85 AS.seek 0.51ml
@05:10:50.17 delay 2 seconds
@05:10:52.20 ATV.dial :bleach
@05:10:52.88 AS.seek 0.81ml
@05:11:53.47 delay 3 seconds
@05:11:56.50 ATV.dial :air3
@05:11:57.10 delay 3 seconds
@05:12:00.18 AS.seek 0.835ml
@05:12:05.46 delay 3 seconds
@05:12:08.49 ATV.dial :bleach
@05:12:09.13 AS.seek 0.82ml
@05:12:12.44 delay 3 seconds
@05:12:15.47 ARV.dial :waste
@05:12:16.10 delay 3 seconds
@05:12:19.13 ATV.dial :ARV
@05:12:20.06 AS.reconfigure ASconfig
@05:12:20.11 AS.seek 0.47ml
@05:12:40.49 AFV.select :carrierSolution
@05:12:40.61 delay 2 seconds
@05:12:42.63 AS.seek 1ml
@05:13:13.41 delay 1 second
@05:13:14.44 AFV.select :holdingCoil
@05:13:14.54 delay 1 second
@05:13:15.56 AS.reconfigure ASfast
@05:13:15.62 AS.seek 0ml
@05:13:42.96 AFV.select :carrierSolution
@05:13:43.08 delay 2 seconds
@05:13:45.10 AS.reconfigure ASconfig
@05:13:45.15 AS.seek 1ml
@05:14:42.88 delay 1 second
@05:14:43.91 AFV.select :holdingCoil
@05:14:44.01 delay 1 second
@05:14:45.03 AS.reconfigure ASfast
@05:14:45.08 AS.seek 0ml
@05:15:12.42 AFV.select :carrierSolution
@05:15:12.54 delay 2 seconds
@05:15:14.56 AS.reconfigure ASconfig
@05:15:14.61 AS.seek 1ml
@05:16:12.36 delay 1 second
@05:16:13.39 AFV.select :holdingCoil
@05:16:13.49 delay 1 second
@05:16:14.51 AS.reconfigure ASfast
@05:16:14.57 AS.seek 0ml
@05:16:42.27 ARV.dial between carrier and air1
@05:16:42.81 ATV.dial :air3
@05:16:44.16 AS.reconfigure ASslow1
@05:16:44.22 AS.seek 0.01ml
@05:16:46.55 delay 2 seconds
@05:16:48.58 ATV.dial :bleach
@05:16:49.22 AS.seek 0.81ml
@05:19:30.46 delay 3 seconds
@05:19:33.49 ATV.dial :air3
@05:19:34.07 delay 3 seconds
@05:19:37.14 AS.seek 0.835ml
@05:19:42.46 delay 3 seconds
@05:19:45.49 ATV.dial :bleach
@05:19:46.13 AS.seek 0.82ml
@05:19:49.42 delay 3 seconds
@05:19:52.45 ATV.dial PCR
@05:19:52.87 AS.reconfigure ASconfig
@05:19:52.92 AS.seek 0ml
@05:20:40.33 PCR.program :bleachProfile
@05:20:40.40 PCR program should complete in < 2:45
@05:20:40.42 PCR.run 10:00
@05:23:36.21 PCR.off
@05:23:36.25 AFV.select :carrierSolution
@05:23:36.36 delay 2 seconds
@05:23:38.38 AS.seek 1ml
@05:24:36.14 delay 1 second
@05:24:37.17 AFV.select :holdingCoil
@05:24:37.27 delay 1 second
@05:24:38.29 AS.reconfigure ASfast
@05:24:38.35 AS.seek 0ml
@05:25:05.69 delay 3 seconds
@05:25:08.72 AFV.select :carrierSolution
@05:25:08.82 delay 2 seconds
@05:25:10.84 AS.reconfigure ASconfig
@05:25:10.91 AS.seek 1ml
@05:26:08.64 delay 1 second
@05:26:09.67 AFV.select :holdingCoil
@05:26:09.77 delay 1 second
@05:26:10.79 AS.reconfigure ASfast
@05:26:10.84 AS.seek 0ml
@05:26:38.16 delay 3 seconds
@05:26:41.19 AFV.select :carrierSolution
@05:26:41.29 delay 2 seconds
@05:26:43.31 AS.reconfigure ASconfig
@05:26:43.37 AS.seek 1ml
@05:27:41.13 delay 1 second
@05:27:42.16 AFV.select :holdingCoil
@05:27:42.26 delay 1 second
@05:27:43.28 AS.reconfigure ASfast
@05:27:43.33 AS.seek 0ml
@05:28:10.64 delay 3 seconds
@05:28:13.67 AFV.select :carrierSolution
@05:28:13.77 delay 2 seconds
@05:28:15.79 AS.reconfigure ASconfig
@05:28:15.86 AS.seek 1ml
@05:29:13.61 delay 1 second
@05:29:14.64 AFV.select :holdingCoil
@05:29:14.74 delay 1 second
@05:29:15.76 AS.reconfigure ASfast
@05:29:15.81 AS.seek 0ml
@05:29:43.14 delay 3 seconds
@05:29:46.17 AFV.select :carrierSolution
@05:29:46.27 delay 2 seconds
@05:29:48.29 AS.reconfigure ASconfig
@05:29:48.35 AS.seek 1ml
@05:30:46.52 delay 1 second
@05:30:47.55 AFV.select :holdingCoil
@05:30:47.65 delay 1 second
@05:30:48.67 AS.reconfigure ASnorm
@05:30:48.72 AS.seek 0ml
@05:31:39.46 <MAIN> AFV.select :carrierSolution
@05:31:39.58 delay 2 seconds
@05:31:41.60 AS.reconfigure ASconfig
@05:31:41.66 AS.seek 0.5ml
@05:32:10.69 delay 1 second
@05:32:11.72 AFV.select :holdingCoil
@05:32:11.82 ATV.dial :air1
@05:32:13.38 AS.reconfigure ASslow2
@05:32:13.43 AS.seek 0.506ml
@05:32:19.69 ATV.dial :extSrc
@05:32:21.47 AS.seek 0.512ml
@05:32:27.80 delay 5 seconds
@05:32:32.83 ATV.dial :air1
@05:32:34.64 AS.reconfigure ASslow1
@05:32:34.69 AS.seek 0.542ml
@05:32:41.08 ATV.dial :waste
@05:32:41.68 AS.reconfigure ASconfig
@05:32:41.74 AS.seek 0ml
@05:33:13.32 <MFBPCR> Setting up PCR for AOPP1 -- writing /var/log/esp/AOPP121may0705h.pcr
@05:33:13.34 Building PCR Slug from extSrc with MM1 mix, PP1 primer
@05:33:13.36 Priming with carrier
@05:33:13.38 ATV.dial ARV
@05:33:14.94 ARV.dial :carrier
@05:33:15.30 AS.reconfigure ASnorm
@05:33:15.37 AS.seek 0.5ml
@05:33:40.94 AFV.select :carrierSolution
@05:33:41.07 delay 2 seconds
@05:33:43.09 AS.reconfigure ASconfig
@05:33:43.15 AS.seek 1ml
@05:34:12.14 delay 1 second
@05:34:13.17 AFV.select :holdingCoil
@05:34:13.27 delay 1 second
@05:34:14.29 ATV.dial :waste
@05:34:16.06 AS.reconfigure ASnorm
@05:34:16.12 AS.seek 0ml
@05:35:06.83 AFV.select :carrierSolution
@05:35:06.96 delay 2 seconds
@05:35:08.98 AS.reconfigure ASconfig
@05:35:09.04 AS.seek 0.5ml
@05:35:38.07 delay 1 second
@05:35:39.10 AFV.select :holdingCoil
@05:35:39.20 Building Slug
@05:35:39.21 ATV.dial :ARV
@05:35:40.97 delay 2 seconds
@05:35:42.99 ARV.dial :air1
@05:35:43.40 delay 2 seconds
@05:35:45.46 AS.reconfigure ASslow2
@05:35:45.52 AS.seek 0.506ml
@05:35:52.16 delay 2 seconds
@05:35:54.19 ARV.dial :MM1
@05:35:54.82 delay 2 seconds
@05:35:56.85 Adding MM1
@05:35:56.92 AS.seek 0.524ml
@05:36:15.38 delay 25 seconds
@05:36:40.41 ARV.dial :PP1
@05:36:40.81 Adding PP1
@05:36:40.82 delay 2 seconds
@05:36:42.90 AS.seek 0.53ml
@05:36:49.38 delay 10 seconds
@05:36:59.42 ARV.dial :air1
@05:36:59.84 adding ARV air
@05:36:59.85 delay 2 seconds
@05:37:01.92 AS.seek 0.536ml
@05:37:08.22 delay 5 seconds
@05:37:13.25 ARV.dial :carrier
@05:37:13.67 delay 7 seconds
@05:37:20.75 AS.seek 0.594ml
@05:38:19.19 delay 5 seconds
@05:38:24.22 ATV.dial :extSrc
@05:38:24.82 delay 2 seconds
@05:38:26.90 AS.seek 0.6ml
@05:38:33.28 delay 4 seconds
@05:38:37.31 ATV.dial ARV
@05:38:37.89 delay 2 seconds
@05:38:39.92 ARV.dial :carrier
@05:38:39.93 delay 2 seconds
@05:38:42.01 AS.seek 0.65ml
@05:39:32.91 delay 4 seconds
@05:39:36.94 ATV.dial PCR
@05:39:37.75 ASV.dial :waste1
@05:39:37.77 ARV.dial :air1
@05:39:38.19 Positioning Slug by 0.324ml
@05:39:38.27 AS.seek 0.326ml
@05:45:04.94 delay 5 seconds
@05:45:09.97 ATV.dial ARV
@05:45:10.78 ASV.dial :PCRlock
@05:45:11.17 delay 5 seconds
@05:45:16.20 Running AOPP1 Profile60CIQ1 thermocycling
@05:45:16.22 <PCR> PCR.program Profile60CIQ1
@05:45:16.33 Profile60CIQ1 should complete in < 1:56:15
@05:45:16.34 PCR.run 2:30:18.75
@07:24:15.80 PCR.off
@07:24:16.37 <MFBPCR> Running AOPP1 MeltProfile60 thermocycling
@07:24:16.38 <PCR> PCR.program MeltProfile60
@07:24:16.49 MeltProfile60 should complete in < 11:55
@07:24:16.50 PCR.run 19:53.75
@07:31:47.94 PCR.off
@07:31:48.86 <MFBPCR> PCR for AOPP1 completed -- closed /var/log/esp/AOPP121may0705h.pcr
@07:31:48.93 Cleaning PCR
@07:31:48.94 AFV.select :carrierSolution
@07:31:49.06 delay 2 seconds
@07:31:51.08 AS.reconfigure ASconfig
@07:31:51.18 AS.seek 0.5ml
@07:32:01.53 delay 1 second
@07:32:02.55 AFV.select :holdingCoil
@07:32:02.68 ASV.dial :waste1
@07:32:03.12 ATV.dial :air3
@07:32:04.57 AS.reconfigure ASslow1
@07:32:04.67 AS.seek 0.51ml
@07:32:07.86 delay 2 seconds
@07:32:09.89 ATV.dial :bleach
@07:32:10.54 AS.seek 0.81ml
@07:33:11.10 delay 3 seconds
@07:33:14.13 ATV.dial :air3
@07:33:14.71 delay 3 seconds
@07:33:17.79 AS.seek 0.835ml
@07:33:23.09 delay 3 seconds
@07:33:26.12 ATV.dial :bleach
@07:33:26.77 AS.seek 0.82ml
@07:33:30.19 delay 3 seconds
@07:33:33.22 ARV.dial :waste
@07:33:33.85 delay 3 seconds
@07:33:36.88 ATV.dial :ARV
@07:33:37.81 AS.reconfigure ASconfig
@07:33:37.86 AS.seek 0.47ml
@07:33:58.29 AFV.select :carrierSolution
@07:33:58.41 delay 2 seconds
@07:34:00.43 AS.seek 1ml
@07:34:31.21 delay 1 second
@07:34:32.24 AFV.select :holdingCoil
@07:34:32.34 delay 1 second
@07:34:33.36 AS.reconfigure ASfast
@07:34:33.42 AS.seek 0ml
@07:35:00.74 AFV.select :carrierSolution
@07:35:00.85 delay 2 seconds
@07:35:02.87 AS.reconfigure ASconfig
@07:35:02.92 AS.seek 1ml
@07:36:00.66 delay 1 second
@07:36:01.69 AFV.select :holdingCoil
@07:36:01.79 delay 1 second
@07:36:02.81 AS.reconfigure ASfast
@07:36:02.87 AS.seek 0ml
@07:36:30.18 AFV.select :carrierSolution
@07:36:30.30 delay 2 seconds
@07:36:32.32 AS.reconfigure ASconfig
@07:36:32.37 AS.seek 1ml
@07:37:30.13 delay 1 second
@07:37:31.16 AFV.select :holdingCoil
@07:37:31.26 delay 1 second
@07:37:32.28 AS.reconfigure ASfast
@07:37:32.33 AS.seek 0ml
@07:37:59.64 ARV.dial between carrier and air1
@07:38:00.17 ATV.dial :air3
@07:38:01.52 AS.reconfigure ASslow1
@07:38:01.58 AS.seek 0.01ml
@07:38:03.88 delay 2 seconds
@07:38:05.91 ATV.dial :bleach
@07:38:06.56 AS.seek 0.81ml
@07:40:47.69 delay 3 seconds
@07:40:50.72 ATV.dial :air3
@07:40:51.30 delay 3 seconds
@07:40:54.37 AS.seek 0.835ml
@07:40:59.64 delay 3 seconds
@07:41:02.67 ATV.dial :bleach
@07:41:03.31 AS.seek 0.82ml
@07:41:06.61 delay 3 seconds
@07:41:09.64 ATV.dial PCR
@07:41:10.06 AS.reconfigure ASconfig
@07:41:10.11 AS.seek 0ml
@07:41:57.52 PCR.program :bleachProfile
@07:41:57.59 PCR program should complete in < 2:45
@07:41:57.60 PCR.run 10:00
@07:44:53.78 PCR.off
@07:44:53.82 AFV.select :carrierSolution
@07:44:53.93 delay 2 seconds
@07:44:55.95 AS.seek 1ml
@07:45:53.74 delay 1 second
@07:45:54.77 AFV.select :holdingCoil
@07:45:54.87 delay 1 second
@07:45:55.89 AS.reconfigure ASfast
@07:45:55.94 AS.seek 0ml
@07:46:23.27 delay 3 seconds
@07:46:26.30 AFV.select :carrierSolution
@07:46:26.40 delay 2 seconds
@07:46:28.42 AS.reconfigure ASconfig
@07:46:28.47 AS.seek 1ml
@07:47:26.21 delay 1 second
@07:47:27.24 AFV.select :holdingCoil
@07:47:27.34 delay 1 second
@07:47:28.36 AS.reconfigure ASfast
@07:47:28.42 AS.seek 0ml
@07:47:55.75 delay 3 seconds
@07:47:58.78 AFV.select :carrierSolution
@07:47:58.88 delay 2 seconds
@07:48:00.90 AS.reconfigure ASconfig
@07:48:00.97 AS.seek 1ml
@07:48:58.72 delay 1 second
@07:48:59.75 AFV.select :holdingCoil
@07:48:59.85 delay 1 second
@07:49:00.87 AS.reconfigure ASfast
@07:49:00.92 AS.seek 0ml
@07:49:28.25 delay 3 seconds
@07:49:31.28 AFV.select :carrierSolution
@07:49:31.38 delay 2 seconds
@07:49:33.40 AS.reconfigure ASconfig
@07:49:33.45 AS.seek 1ml
@07:50:31.22 delay 1 second
@07:50:32.25 AFV.select :holdingCoil
@07:50:32.35 delay 1 second
@07:50:33.37 AS.reconfigure ASfast
@07:50:33.43 AS.seek 0ml
@07:51:00.77 delay 3 seconds
@07:51:03.80 AFV.select :carrierSolution
@07:51:03.90 delay 2 seconds
@07:51:05.92 AS.reconfigure ASconfig
@07:51:05.99 AS.seek 1ml
@07:52:03.72 delay 1 second
@07:52:04.75 AFV.select :holdingCoil
@07:52:04.85 delay 1 second
@07:52:05.87 AS.reconfigure ASnorm
@07:52:05.92 AS.seek 0ml
@07:52:56.69 <MAIN> deconEluateRecoveryLine extSrc
ATV.dial :waste
@07:52:58.00 AS.seek 0ml
@07:52:58.07 ATV.dial :air3
@07:52:58.95 AS.reconfigure ASslow1
@07:52:59.00 AS.seek 0.01ml
@07:53:01.32 delay 2 seconds
@07:53:03.35 ATV.dial :bleach
@07:53:03.81 AS.seek 0.31ml
@07:54:04.43 delay 3 seconds
@07:54:07.46 ATV.dial :air3
@07:54:08.04 delay 3 seconds
@07:54:11.11 AS.seek 0.335ml
@07:54:16.39 delay 3 seconds
@07:54:19.42 ATV.dial :bleach
@07:54:20.06 AS.seek 0.32ml
@07:54:23.47 delay 3 seconds
@07:54:26.50 ATV.dial :extSrc
@07:54:27.15 AS.seek 0ml
@07:55:31.89 AFV.select :carrierSolution
@07:55:32.01 delay 2 seconds
@07:55:34.03 AS.reconfigure ASconfig
@07:55:34.08 AS.seek 0.5ml
@07:56:03.08 delay 1 second
@07:56:04.11 AFV.select :holdingCoil
@07:56:04.21 ATV.dial :extSrc
@07:56:04.23 AS.reconfigure ASslow1
@07:56:04.29 AS.seek 0ml
@07:57:45.22 AFV.select :carrierSolution
@07:57:45.34 delay 2 seconds
@07:57:47.36 AS.reconfigure ASconfig
@07:57:47.41 AS.seek 0.5ml
@07:58:16.43 delay 1 second
@07:58:17.46 AFV.select :holdingCoil
@07:58:17.56 ATV.dial :extSrc
@07:58:17.58 AS.reconfigure ASslow1
@07:58:17.64 AS.seek 0ml
@07:59:58.55 AFV.select :carrierSolution
@07:59:58.67 delay 2 seconds
@08:00:00.69 AS.reconfigure ASconfig
@08:00:00.74 AS.seek 0.5ml
@08:00:29.75 delay 1 second
@08:00:30.78 AFV.select :holdingCoil
@08:00:30.88 ATV.dial :extSrc
@08:00:30.90 AS.reconfigure ASslow1
@08:00:30.96 AS.seek 0ml
@08:02:11.75 AFV.select :carrierSolution
@08:02:11.87 delay 2 seconds
@08:02:13.89 AS.reconfigure ASconfig
@08:02:13.94 AS.seek 0.5ml
@08:02:42.97 delay 1 second
@08:02:43.99 AFV.select :holdingCoil
@08:02:44.09 ATV.dial :extSrc
@08:02:44.11 AS.reconfigure ASslow1
@08:02:44.17 AS.seek 0ml
@08:04:25.00 ATV.dial :air2
@08:04:26.39 AS.reconfigure ASslow0
@08:04:26.44 AS.seek 0.5ml
@08:05:17.08 ATV.dial :extSrc
@08:05:18.63 AS.seek 0.02ml
@08:06:07.19 delay 1 second
@08:06:08.22 ATV.dial :waste
@08:06:09.58 AS.reconfigure ASconfig
@08:06:09.63 AS.seek 0ml
@08:06:11.07 deconEluateRecoveryLine extSrc
@08:06:11.08 ATV.dial :waste
@08:06:11.11 AS.reconfigure ASnorm
@08:06:11.17 AS.seek 0ml
@08:06:11.30 ATV.dial :air3
@08:06:12.17 AS.reconfigure ASslow1
@08:06:12.23 AS.seek 0.01ml
@08:06:14.55 delay 2 seconds
@08:06:16.58 ATV.dial :bleach
@08:06:17.05 AS.seek 0.31ml
@08:07:17.75 delay 3 seconds
@08:07:20.78 ATV.dial :air3
@08:07:21.36 delay 3 seconds
@08:07:24.43 AS.seek 0.335ml
@08:07:30.14 delay 3 seconds
@08:07:33.17 ATV.dial :bleach
@08:07:33.81 AS.seek 0.32ml
@08:07:37.11 delay 3 seconds
@08:07:40.14 ATV.dial :extSrc
@08:07:40.78 AS.seek 0ml
@08:08:45.50 AFV.select :carrierSolution
@08:08:45.62 delay 2 seconds
@08:08:47.64 AS.reconfigure ASconfig
@08:08:47.70 AS.seek 0.5ml
@08:09:16.72 delay 1 second
@08:09:17.75 AFV.select :holdingCoil
@08:09:17.85 ATV.dial :extSrc
@08:09:17.87 AS.reconfigure ASslow1
@08:09:17.94 AS.seek 0ml
@08:10:58.75 AFV.select :carrierSolution
@08:10:58.87 delay 2 seconds
@08:11:00.89 AS.reconfigure ASconfig
@08:11:00.94 AS.seek 0.5ml
@08:11:29.96 delay 1 second
@08:11:30.99 AFV.select :holdingCoil
@08:11:31.09 ATV.dial :extSrc
@08:11:31.11 AS.reconfigure ASslow1
@08:11:31.18 AS.seek 0ml
@08:13:12.00 AFV.select :carrierSolution
@08:13:12.12 delay 2 seconds
@08:13:14.14 AS.reconfigure ASconfig
@08:13:14.19 AS.seek 0.5ml
@08:13:43.22 delay 1 second
@08:13:44.25 AFV.select :holdingCoil
@08:13:44.35 ATV.dial :extSrc
@08:13:44.37 AS.reconfigure ASslow1
@08:13:44.43 AS.seek 0ml
@08:15:25.25 AFV.select :carrierSolution
@08:15:25.37 delay 2 seconds
@08:15:27.39 AS.reconfigure ASconfig
@08:15:27.44 AS.seek 0.5ml
@08:15:56.49 delay 1 second
@08:15:57.52 AFV.select :holdingCoil
@08:15:57.62 ATV.dial :extSrc
@08:15:57.64 AS.reconfigure ASslow1
@08:15:57.70 AS.seek 0ml
@08:17:38.58 ATV.dial :air2
@08:17:39.97 AS.reconfigure ASslow0
@08:17:40.03 AS.seek 0.5ml
@08:18:30.66 ATV.dial :extSrc
@08:18:32.24 AS.seek 0.02ml
@08:19:20.86 delay 1 second
@08:19:21.89 ATV.dial :waste
@08:19:23.25 AS.reconfigure ASconfig
@08:19:23.30 AS.seek 0ml
@08:19:24.72 deconEluateRecoveryLine extSrc
@08:19:24.74 ATV.dial :waste
@08:19:24.76 AS.reconfigure ASnorm
@08:19:24.82 AS.seek 0ml
@08:19:24.89 ATV.dial :air3
@08:19:25.77 AS.reconfigure ASslow1
@08:19:25.82 AS.seek 0.01ml
@08:19:28.25 delay 2 seconds
@08:19:30.28 ATV.dial :bleach
@08:19:30.75 AS.seek 0.31ml
@08:20:31.36 delay 3 seconds
@08:20:34.39 ATV.dial :air3
@08:20:34.97 delay 3 seconds
@08:20:38.04 AS.seek 0.335ml
@08:20:43.26 delay 3 seconds
@08:20:46.29 ATV.dial :bleach
@08:20:46.92 AS.seek 0.32ml
@08:20:50.21 delay 3 seconds
@08:20:53.24 ATV.dial :extSrc
@08:20:53.89 AS.seek 0ml
@08:21:58.50 AFV.select :carrierSolution
@08:21:58.62 delay 2 seconds
@08:22:00.64 AS.reconfigure ASconfig
@08:22:00.69 AS.seek 0.5ml
@08:22:29.72 delay 1 second
@08:22:30.75 AFV.select :holdingCoil
@08:22:30.85 ATV.dial :extSrc
@08:22:30.87 AS.reconfigure ASslow1
@08:22:30.93 AS.seek 0ml
@08:24:11.74 AFV.select :carrierSolution
@08:24:11.86 delay 2 seconds
@08:24:13.88 AS.reconfigure ASconfig
@08:24:13.93 AS.seek 0.5ml
@08:24:42.97 delay 1 second
@08:24:43.99 AFV.select :holdingCoil
@08:24:44.09 ATV.dial :extSrc
@08:24:44.11 AS.reconfigure ASslow1
@08:24:44.17 AS.seek 0ml
@08:26:25.13 AFV.select :carrierSolution
@08:26:25.25 delay 2 seconds
@08:26:27.27 AS.reconfigure ASconfig
@08:26:27.32 AS.seek 0.5ml
@08:26:56.33 delay 1 second
@08:26:57.36 AFV.select :holdingCoil
@08:26:57.46 ATV.dial :extSrc
@08:26:57.48 AS.reconfigure ASslow1
@08:26:57.54 AS.seek 0ml
@08:28:38.47 AFV.select :carrierSolution
@08:28:38.59 delay 2 seconds
@08:28:40.61 AS.reconfigure ASconfig
@08:28:40.66 AS.seek 0.5ml
@08:29:09.69 delay 1 second
@08:29:10.72 AFV.select :holdingCoil
@08:29:10.82 ATV.dial :extSrc
@08:29:10.84 AS.reconfigure ASslow1
@08:29:10.90 AS.seek 0ml
@08:30:51.72 ATV.dial :air2
@08:30:53.11 AS.reconfigure ASslow0
@08:30:53.16 AS.seek 0.5ml
@08:31:43.79 ATV.dial :extSrc
@08:31:45.36 AS.seek 0.02ml
@08:32:33.97 delay 1 second
@08:32:35.00 ATV.dial :waste
@08:32:36.36 AS.reconfigure ASconfig
@08:32:36.42 AS.seek 0ml
@08:32:37.88 <mwj> Session Ended
@15:58:40.73CEST07-May-21 <MAIN> ESP2 v3.30 I2C Gateway v3.06 for core bus
@15:58:43.26 
Configuration for mer 7/20/20 brent@mbari.org
/home/esp/esp2/type/shallow/mer/configure.rb
@15:58:43.35 
Configuration for MFB3 by preston@mbari.org
/home/esp/esp2/type/mfb/MFB3/configure.rb
@15:58:47.69 Polling Can every 10:00
@15:58:49.40 CS.configure :default
@15:58:49.44 CS.hold
@15:58:49.51 CS.configure :default
@15:58:49.59 PS.configure :default
@15:58:49.67 SS.configure :default
@15:58:49.71 SS.hold
@15:58:49.76 SS.configure :default
@15:58:49.84 CC.configure :default
@15:58:49.87 CC.hold
@15:58:49.94 CC.configure :default
@15:58:50.02 PC.configure :default
@15:58:50.06 PC.hold
@15:58:50.12 PC.configure :default
@15:58:50.22 Forearm.configure :loose
@15:58:50.26 Forearm.hold
@15:58:50.31 Forearm.configure :loose
@15:58:50.39 Elbow.configure :loose
@15:58:50.43 Elbow.hold
@15:58:50.48 Elbow.configure :loose
@15:58:50.58 SC.configure :loose
@15:58:50.62 SC.hold
@15:58:50.66 SC.configure :loose
@15:58:50.74 SE.configure :default
@15:58:50.78 SE.hold
@15:58:50.82 SE.configure :default
@15:58:50.86 CH.coast
@15:58:50.90 CH.configure :default
@15:58:50.93 PH.coast
@15:58:50.98 PH.configure :default
@15:58:51.07 SPE.coast
@15:58:51.12 SPE.configure SPEconfig
@15:58:51.19 AS.configure ASconfig
@15:58:51.23 AS.hold
@15:58:51.27 AS.configure ASconfig
@15:58:51.32 PCR.off
@15:58:51.35 PCR.pidtemp= 50,0.1,0,300,25,25
@15:58:51.38 PCR.caltemp= 2.5051,0.0372,0
@15:58:51.41 All dwarf controllers running firmware version 3.75
@15:58:51.65 Cleanly exited at 15:58:06CEST07-May-21
@15:58:51.68 Loading /home/esp/esp2/mission/stdMER2017.rb mission
@15:58:52.18 AFV.select :carrierSolution
@15:58:52.28 delay 2 seconds
@15:58:54.30 AS.seek 0.5ml
@15:59:23.30 delay 1 second
@15:59:24.32 AFV.select :holdingCoil
@15:59:24.41 ATV.dial :extSrc
@15:59:25.80 AS.reconfigure ASslow1
@15:59:25.84 AS.seek 0.53ml
@15:59:32.16 delay 5 seconds
@15:59:32.72 <mwj> Session Begun
@15:59:37.24 <MAIN> AS.reconfigure ASslow2
@15:59:37.29 AS.seek 0.54ml
@15:59:47.70 delay 20 seconds
@16:00:07.76 AS.reconfigure ASslow1
@16:00:07.82 AS.seek 0.57ml
@16:00:14.17 delay 5 seconds
@16:00:19.24 AS.reconfigure ASslow2
@16:00:19.30 AS.seek 0.58ml
@16:00:29.83 delay 20 seconds
@16:00:49.89 AS.reconfigure ASslow1
@16:00:49.95 AS.seek 0.61ml
@16:00:56.34 delay 5 seconds
@16:01:01.41 AS.reconfigure ASslow2
@16:01:01.46 AS.seek 0.62ml
@16:01:11.98 delay 20 seconds
@16:01:32.04 AS.reconfigure ASslow1
@16:01:32.11 AS.seek 0.65ml
@16:01:38.45 delay 5 seconds
@16:01:43.52 AS.reconfigure ASslow2
@16:01:43.58 AS.seek 0.66ml
@16:01:54.14 delay 20 seconds
@16:02:14.16 AS.reconfigure ASslow1
@16:02:14.22 AS.seek 0.705ml
@16:02:23.56 delay 10 seconds
@16:02:33.58 ATV.dial :air1
@16:02:35.75 AS.seek 0.725ml
@16:02:40.14 delay 3 seconds
@16:02:43.16 ATV.dial :waste
@16:02:43.75 AS.reconfigure ASslow0
@16:02:43.80 AS.seek 0ml
@16:03:57.11 <MFBPCR> Setting up PCR for ACYPP2 -- writing /var/log/esp/ACYPP221may0716h.pcr
@16:03:57.13 Building PCR Slug from extSrc with MM1 mix, PP2 primer
@16:03:57.16 Priming with carrier
@16:03:57.18 ATV.dial ARV
@16:03:58.77 ARV.dial :carrier
@16:03:59.13 AS.reconfigure ASnorm
@16:03:59.20 AS.seek 0.5ml
@16:04:24.71 AFV.select :carrierSolution
@16:04:24.84 delay 2 seconds
@16:04:26.86 AS.reconfigure ASconfig
@16:04:26.93 AS.seek 1ml
@16:04:55.97 delay 1 second
@16:04:56.99 AFV.select :holdingCoil
@16:04:57.09 delay 1 second
@16:04:58.11 ATV.dial :waste
@16:04:59.89 AS.reconfigure ASnorm
@16:04:59.96 AS.seek 0ml
@16:05:50.65 AFV.select :carrierSolution
@16:05:50.77 delay 2 seconds
@16:05:52.79 AS.reconfigure ASconfig
@16:05:52.85 AS.seek 0.5ml
@16:06:21.86 delay 1 second
@16:06:22.88 AFV.select :holdingCoil
@16:06:22.98 Building Slug
@16:06:22.99 ATV.dial :ARV
@16:06:24.77 delay 2 seconds
@16:06:26.79 ARV.dial :air1
@16:06:27.20 delay 2 seconds
@16:06:29.27 AS.reconfigure ASslow2
@16:06:29.32 AS.seek 0.506ml
@16:06:35.86 delay 2 seconds
@16:06:37.88 ARV.dial :MM1
@16:06:38.53 delay 2 seconds
@16:06:40.55 Adding MM1
@16:06:40.62 AS.seek 0.524ml
@16:06:59.08 delay 25 seconds
@16:07:24.10 ARV.dial :PP2
@16:07:24.52 Adding PP2
@16:07:24.53 delay 2 seconds
@16:07:26.61 AS.seek 0.53ml
@16:07:32.97 delay 10 seconds
@16:07:43.01 ARV.dial :air2
@16:07:43.44 adding ARV air
@16:07:43.45 delay 2 seconds
@16:07:45.53 AS.seek 0.536ml
@16:07:52.15 delay 5 seconds
@16:07:57.18 ARV.dial :carrier
@16:07:58.55 delay 7 seconds
@16:08:05.62 AS.seek 0.594ml
@16:09:04.27 delay 5 seconds
@16:09:09.29 ATV.dial :extSrc
@16:09:09.89 delay 2 seconds
@16:09:11.96 AS.seek 0.6ml
@16:09:18.33 delay 4 seconds
@16:09:22.36 ATV.dial ARV
@16:09:22.94 delay 2 seconds
@16:09:24.96 ARV.dial :carrier
@16:09:24.97 delay 2 seconds
@16:09:27.05 AS.seek 0.65ml
@16:10:17.78 delay 4 seconds
@16:10:21.80 ATV.dial PCR
@16:10:22.60 ASV.dial :waste1
@16:10:22.70 ARV.dial :air1
@16:10:23.11 Positioning Slug by 0.324ml
@16:10:23.20 AS.seek 0.326ml
@16:15:49.31 delay 5 seconds
@16:15:54.33 ATV.dial ARV
@16:15:55.15 ASV.dial :PCRlock
@16:15:55.56 delay 5 seconds
@16:16:00.58 Running ACYPP2 Profile60CIQ2 thermocycling
@16:16:00.60 <PCR> PCR.program Profile60CIQ2
@16:16:00.71 Profile60CIQ2 should complete in < 1:47:50
@16:16:00.72 PCR.run 2:19:47.5
@17:44:41.66 PCR.off
@17:44:42.26 <MFBPCR> Running ACYPP2 MeltProfile60 thermocycling
@17:44:42.28 <PCR> PCR.program MeltProfile60
@17:44:42.38 MeltProfile60 should complete in < 11:55
@17:44:42.39 PCR.run 19:53.75
@17:52:17.02 PCR.off
@17:52:17.90 <MFBPCR> PCR for ACYPP2 completed -- closed /var/log/esp/ACYPP221may0716h.pcr
@17:52:17.98 Cleaning PCR
@17:52:17.99 AFV.select :carrierSolution
@17:52:18.11 delay 2 seconds
@17:52:20.13 AS.reconfigure ASconfig
@17:52:20.19 AS.seek 0.5ml
@17:52:30.52 delay 1 second
@17:52:31.55 AFV.select :holdingCoil
@17:52:31.65 ASV.dial :waste1
@17:52:32.05 ATV.dial :air3
@17:52:33.41 AS.reconfigure ASslow1
@17:52:33.47 AS.seek 0.51ml
@17:52:35.78 delay 2 seconds
@17:52:37.80 ATV.dial :bleach
@17:52:38.80 AS.seek 0.81ml
@17:53:39.55 delay 3 seconds
@17:53:42.57 ATV.dial :air3
@17:53:43.16 delay 3 seconds
@17:53:46.23 AS.seek 0.835ml
@17:53:51.55 delay 3 seconds
@17:53:54.58 ATV.dial :bleach
@17:53:55.22 AS.seek 0.82ml
@17:53:58.51 delay 3 seconds
@17:54:01.53 ARV.dial :waste
@17:54:02.19 delay 3 seconds
@17:54:05.21 ATV.dial :ARV
@17:54:06.14 AS.reconfigure ASconfig
@17:54:06.20 AS.seek 0.47ml
@17:54:26.60 AFV.select :carrierSolution
@17:54:26.72 delay 2 seconds
@17:54:28.74 AS.seek 1ml
@17:54:59.47 delay 1 second
@17:55:00.50 AFV.select :holdingCoil
@17:55:00.61 delay 1 second
@17:55:01.63 AS.reconfigure ASfast
@17:55:01.69 AS.seek 0ml
@17:55:29.00 AFV.select :carrierSolution
@17:55:29.12 delay 2 seconds
@17:55:31.14 AS.reconfigure ASconfig
@17:55:31.20 AS.seek 1ml
@17:56:28.98 delay 1 second
@17:56:30.00 AFV.select :holdingCoil
@17:56:30.10 delay 1 second
@17:56:31.12 AS.reconfigure ASfast
@17:56:31.18 AS.seek 0ml
@17:56:58.50 AFV.select :carrierSolution
@17:56:58.62 delay 2 seconds
@17:57:00.64 AS.reconfigure ASconfig
@17:57:00.70 AS.seek 1ml
@17:57:58.44 delay 1 second
@17:57:59.46 AFV.select :holdingCoil
@17:57:59.56 delay 1 second
@17:58:00.58 AS.reconfigure ASfast
@17:58:00.64 AS.seek 0ml
@17:58:27.94 ARV.dial between carrier and air1
@17:58:28.47 ATV.dial :air3
@17:58:29.81 AS.reconfigure ASslow1
@17:58:29.87 AS.seek 0.01ml
@17:58:32.18 delay 2 seconds
@17:58:34.21 ATV.dial :bleach
@17:58:34.85 AS.seek 0.81ml
@18:01:16.09 delay 3 seconds
@18:01:19.11 ATV.dial :air3
@18:01:19.70 delay 3 seconds
@18:01:22.77 AS.seek 0.835ml
@18:01:28.16 delay 3 seconds
@18:01:31.19 ATV.dial :bleach
@18:01:31.83 AS.seek 0.82ml
@18:01:35.24 delay 3 seconds
@18:01:38.27 ATV.dial PCR
@18:01:38.69 AS.reconfigure ASconfig
@18:01:38.74 AS.seek 0ml
@18:02:26.18 PCR.program :bleachProfile
@18:02:26.25 PCR program should complete in < 2:45
@18:02:26.27 PCR.run 10:00
@18:05:22.05 PCR.off
@18:05:22.09 AFV.select :carrierSolution
@18:05:22.20 delay 2 seconds
@18:05:24.22 AS.seek 1ml
@18:06:21.97 delay 1 second
@18:06:23.00 AFV.select :holdingCoil
@18:06:23.10 delay 1 second
@18:06:24.12 AS.reconfigure ASfast
@18:06:24.18 AS.seek 0ml
@18:06:51.51 delay 3 seconds
@18:06:54.53 AFV.select :carrierSolution
@18:06:54.63 delay 2 seconds
@18:06:56.65 AS.reconfigure ASconfig
@18:06:56.72 AS.seek 1ml
@18:07:54.51 delay 1 second
@18:07:55.53 AFV.select :holdingCoil
@18:07:55.63 delay 1 second
@18:07:56.65 AS.reconfigure ASfast
@18:07:56.71 AS.seek 0ml
@18:08:24.05 delay 3 seconds
@18:08:27.07 AFV.select :carrierSolution
@18:08:27.17 delay 2 seconds
@18:08:29.19 AS.reconfigure ASconfig
@18:08:29.25 AS.seek 1ml
@18:09:27.02 delay 1 second
@18:09:28.05 AFV.select :holdingCoil
@18:09:28.15 delay 1 second
@18:09:29.17 AS.reconfigure ASfast
@18:09:29.23 AS.seek 0ml
@18:09:56.97 delay 3 seconds
@18:09:59.99 AFV.select :carrierSolution
@18:10:00.09 delay 2 seconds
@18:10:02.11 AS.reconfigure ASconfig
@18:10:02.17 AS.seek 1ml
@18:10:59.97 delay 1 second
@18:11:00.99 AFV.select :holdingCoil
@18:11:01.09 delay 1 second
@18:11:02.11 AS.reconfigure ASfast
@18:11:02.17 AS.seek 0ml
@18:11:29.51 delay 3 seconds
@18:11:32.53 AFV.select :carrierSolution
@18:11:32.63 delay 2 seconds
@18:11:34.65 AS.reconfigure ASconfig
@18:11:34.71 AS.seek 1ml
@18:12:32.50 delay 1 second
@18:12:33.53 AFV.select :holdingCoil
@18:12:33.63 delay 1 second
@18:12:34.65 AS.reconfigure ASnorm
@18:12:34.70 AS.seek 0ml
@18:13:25.42 <MAIN> AFV.select :carrierSolution
@18:13:25.53 delay 2 seconds
@18:13:27.55 AS.reconfigure ASconfig
@18:13:27.61 AS.seek 0.5ml
@18:13:56.67 delay 1 second
@18:13:57.69 AFV.select :holdingCoil
@18:13:57.79 ATV.dial :air1
@18:13:59.36 AS.reconfigure ASslow2
@18:13:59.41 AS.seek 0.506ml
@18:14:05.98 ATV.dial :extSrc
@18:14:07.75 AS.seek 0.512ml
@18:14:13.92 delay 5 seconds
@18:14:18.94 ATV.dial :air1
@18:14:20.75 AS.reconfigure ASslow1
@18:14:20.80 AS.seek 0.542ml
@18:14:27.20 ATV.dial :waste
@18:14:27.80 AS.reconfigure ASconfig
@18:14:27.85 AS.seek 0ml
@18:14:59.43 <MFBPCR> Setting up PCR for ACYPP2 -- writing /var/log/esp/ACYPP221may0718h.pcr
@18:14:59.46 Building PCR Slug from extSrc with MM1 mix, PP2 primer
@18:14:59.48 Priming with carrier
@18:14:59.50 ATV.dial ARV
@18:15:01.08 ARV.dial :carrier
@18:15:01.42 AS.reconfigure ASnorm
@18:15:01.49 AS.seek 0.5ml
@18:15:26.99 AFV.select :carrierSolution
@18:15:27.11 delay 2 seconds
@18:15:29.13 AS.reconfigure ASconfig
@18:15:29.20 AS.seek 1ml
@18:15:58.23 delay 1 second
@18:15:59.25 AFV.select :holdingCoil
@18:15:59.35 delay 1 second
@18:16:00.37 ATV.dial :waste
@18:16:02.15 AS.reconfigure ASnorm
@18:16:02.21 AS.seek 0ml
@18:16:52.93 AFV.select :carrierSolution
@18:16:53.05 delay 2 seconds
@18:16:55.07 AS.reconfigure ASconfig
@18:16:55.13 AS.seek 0.5ml
@18:17:24.17 delay 1 second
@18:17:25.19 AFV.select :holdingCoil
@18:17:25.29 Building Slug
@18:17:25.30 ATV.dial :ARV
@18:17:27.06 delay 2 seconds
@18:17:29.08 ARV.dial :air1
@18:17:29.49 delay 2 seconds
@18:17:31.55 AS.reconfigure ASslow2
@18:17:31.62 AS.seek 0.506ml
@18:17:37.90 delay 2 seconds
@18:17:39.92 ARV.dial :MM1
@18:17:40.58 delay 2 seconds
@18:17:42.60 Adding MM1
@18:17:42.67 AS.seek 0.524ml
@18:18:01.20 delay 25 seconds
@18:18:26.22 ARV.dial :PP2
@18:18:26.64 Adding PP2
@18:18:26.65 delay 2 seconds
@18:18:28.73 AS.seek 0.53ml
@18:18:34.88 delay 10 seconds
@18:18:44.92 ARV.dial :air2
@18:18:45.34 adding ARV air
@18:18:45.35 delay 2 seconds
@18:18:47.43 AS.seek 0.536ml
@18:18:54.07 delay 5 seconds
@18:18:59.11 ARV.dial :carrier
@18:19:00.48 delay 7 seconds
@18:19:07.69 AS.seek 0.594ml
@18:20:06.28 delay 5 seconds
@18:20:11.31 ATV.dial :extSrc
@18:20:11.92 delay 2 seconds
@18:20:13.99 AS.seek 0.6ml
@18:20:20.91 delay 4 seconds
@18:20:24.94 ATV.dial ARV
@18:20:25.53 delay 2 seconds
@18:20:27.55 ARV.dial :carrier
@18:20:27.56 delay 2 seconds
@18:20:29.64 AS.seek 0.65ml
@18:21:20.43 delay 4 seconds
@18:21:24.46 ATV.dial PCR
@18:21:25.28 ASV.dial :waste1
@18:21:25.29 ARV.dial :air1
@18:21:25.73 Positioning Slug by 0.324ml
@18:21:25.82 AS.seek 0.326ml
@18:26:52.00 delay 5 seconds
@18:26:57.02 ATV.dial ARV
@18:26:57.84 ASV.dial :PCRlock
@18:26:58.25 delay 5 seconds
@18:27:03.27 Running ACYPP2 Profile60CIQ2 thermocycling
@18:27:03.29 <PCR> PCR.program Profile60CIQ2
@18:27:03.41 Profile60CIQ2 should complete in < 1:47:50
@18:27:03.42 PCR.run 2:19:47.5
@19:55:46.74 PCR.off
@19:55:47.36 <MFBPCR> Running ACYPP2 MeltProfile60 thermocycling
@19:55:47.37 <PCR> PCR.program MeltProfile60
@19:55:47.48 MeltProfile60 should complete in < 11:55
@19:55:47.49 PCR.run 19:53.75
@20:03:22.35 PCR.off
@20:03:23.28 <MFBPCR> PCR for ACYPP2 completed -- closed /var/log/esp/ACYPP221may0718h.pcr
@20:03:23.35 Cleaning PCR
@20:03:23.36 AFV.select :carrierSolution
@20:03:23.48 delay 2 seconds
@20:03:25.50 AS.reconfigure ASconfig
@20:03:25.56 AS.seek 0.5ml
@20:03:35.87 delay 1 second
@20:03:36.89 AFV.select :holdingCoil
@20:03:36.99 ASV.dial :waste1
@20:03:37.39 ATV.dial :air3
@20:03:38.74 AS.reconfigure ASslow1
@20:03:38.80 AS.seek 0.51ml
@20:03:41.09 delay 2 seconds
@20:03:43.11 ATV.dial :bleach
@20:03:43.75 AS.seek 0.81ml
@20:04:44.45 delay 3 seconds
@20:04:47.47 ATV.dial :air3
@20:04:48.06 delay 3 seconds
@20:04:51.13 AS.seek 0.835ml
@20:04:56.54 delay 3 seconds
@20:04:59.57 ATV.dial :bleach
@20:05:00.20 AS.seek 0.82ml
@20:05:03.60 delay 3 seconds
@20:05:06.63 ARV.dial :waste
@20:05:07.28 delay 3 seconds
@20:05:10.30 ATV.dial :ARV
@20:05:11.22 AS.reconfigure ASconfig
@20:05:11.27 AS.seek 0.47ml
@20:05:31.71 AFV.select :carrierSolution
@20:05:31.83 delay 2 seconds
@20:05:33.85 AS.seek 1ml
@20:06:04.63 delay 1 second
@20:06:05.66 AFV.select :holdingCoil
@20:06:05.77 delay 1 second
@20:06:06.79 AS.reconfigure ASfast
@20:06:06.85 AS.seek 0ml
@20:06:34.21 AFV.select :carrierSolution
@20:06:34.33 delay 2 seconds
@20:06:36.35 AS.reconfigure ASconfig
@20:06:36.41 AS.seek 1ml
@20:07:34.14 delay 1 second
@20:07:35.17 AFV.select :holdingCoil
@20:07:35.27 delay 1 second
@20:07:36.29 AS.reconfigure ASfast
@20:07:36.34 AS.seek 0ml
@20:08:03.68 AFV.select :carrierSolution
@20:08:03.80 delay 2 seconds
@20:08:05.82 AS.reconfigure ASconfig
@20:08:05.87 AS.seek 1ml
@20:09:03.66 delay 1 second
@20:09:04.69 AFV.select :holdingCoil
@20:09:04.79 delay 1 second
@20:09:05.81 AS.reconfigure ASfast
@20:09:05.87 AS.seek 0ml
@20:09:33.20 ARV.dial between carrier and air1
@20:09:33.73 ATV.dial :air3
@20:09:35.09 AS.reconfigure ASslow1
@20:09:35.15 AS.seek 0.01ml
@20:09:37.49 delay 2 seconds
@20:09:39.52 ATV.dial :bleach
@20:09:40.17 AS.seek 0.81ml
@20:12:21.29 delay 3 seconds
@20:12:24.31 ATV.dial :air3
@20:12:24.90 delay 3 seconds
@20:12:27.97 AS.seek 0.835ml
@20:12:33.25 delay 3 seconds
@20:12:36.28 ATV.dial :bleach
@20:12:36.92 AS.seek 0.82ml
@20:12:40.22 delay 3 seconds
@20:12:43.25 ATV.dial PCR
@20:12:43.67 AS.reconfigure ASconfig
@20:12:43.72 AS.seek 0ml
@20:13:31.14 PCR.program :bleachProfile
@20:13:31.21 PCR program should complete in < 2:45
@20:13:31.22 PCR.run 10:00
@20:16:27.41 PCR.off
@20:16:27.45 AFV.select :carrierSolution
@20:16:27.56 delay 2 seconds
@20:16:29.58 AS.seek 1ml
@20:17:27.36 delay 1 second
@20:17:28.38 AFV.select :holdingCoil
@20:17:28.48 delay 1 second
@20:17:29.50 AS.reconfigure ASfast
@20:17:29.56 AS.seek 0ml
@20:17:56.90 delay 3 seconds
@20:17:59.92 AFV.select :carrierSolution
@20:18:00.02 delay 2 seconds
@20:18:02.04 AS.reconfigure ASconfig
@20:18:02.10 AS.seek 1ml
@20:18:59.85 delay 1 second
@20:19:00.88 AFV.select :holdingCoil
@20:19:00.98 delay 1 second
@20:19:02.00 AS.reconfigure ASfast
@20:19:02.06 AS.seek 0ml
@20:19:29.40 delay 3 seconds
@20:19:32.42 AFV.select :carrierSolution
@20:19:32.52 delay 2 seconds
@20:19:34.54 AS.reconfigure ASconfig
@20:19:34.60 AS.seek 1ml
@20:20:32.40 delay 1 second
@20:20:33.42 AFV.select :holdingCoil
@20:20:33.52 delay 1 second
@20:20:34.54 AS.reconfigure ASfast
@20:20:34.60 AS.seek 0ml
@20:21:01.95 delay 3 seconds
@20:21:04.97 AFV.select :carrierSolution
@20:21:05.07 delay 2 seconds
@20:21:07.09 AS.reconfigure ASconfig
@20:21:07.15 AS.seek 1ml
@20:22:04.93 delay 1 second
@20:22:05.95 AFV.select :holdingCoil
@20:22:06.05 delay 1 second
@20:22:07.07 AS.reconfigure ASfast
@20:22:07.13 AS.seek 0ml
@20:22:34.48 delay 3 seconds
@20:22:37.50 AFV.select :carrierSolution
@20:22:37.60 delay 2 seconds
@20:22:39.62 AS.reconfigure ASconfig
@20:22:39.68 AS.seek 1ml
@20:23:37.50 delay 1 second
@20:23:38.52 AFV.select :holdingCoil
@20:23:38.62 delay 1 second
@20:23:39.64 AS.reconfigure ASnorm
@20:23:39.70 AS.seek 0ml
@20:24:30.42 <MAIN> AFV.select :carrierSolution
@20:24:30.52 delay 2 seconds
@20:24:32.54 AS.reconfigure ASconfig
@20:24:32.59 AS.seek 0.5ml
@20:25:01.61 delay 1 second
@20:25:02.63 AFV.select :holdingCoil
@20:25:02.73 ATV.dial :air1
@20:25:04.31 AS.reconfigure ASslow2
@20:25:04.36 AS.seek 0.51ml
@20:25:14.81 delay 5 seconds
@20:25:19.83 ATV.dial :extSrc
@20:25:21.63 AS.seek 0.53ml
@20:25:42.45 delay 10 seconds
@20:25:52.47 ATV.dial :air1
@20:25:54.27 AS.reconfigure ASslow1
@20:25:54.32 AS.seek 0.56ml
@20:26:00.61 ATV.dial :waste
@20:26:01.19 AS.reconfigure ASconfig
@20:26:01.25 AS.seek 0ml
@20:26:33.87 <MFBPCR> Setting up PCR for ACYPP2 -- writing /var/log/esp/ACYPP221may0720h.pcr
@20:26:33.89 Building PCR Slug from extSrc with MM1 mix, PP2 primer
@20:26:33.91 Priming with carrier
@20:26:33.93 ATV.dial ARV
@20:26:35.51 ARV.dial :carrier
@20:26:35.84 AS.reconfigure ASnorm
@20:26:35.90 AS.seek 0.5ml
@20:27:01.42 AFV.select :carrierSolution
@20:27:01.55 delay 2 seconds
@20:27:03.57 AS.reconfigure ASconfig
@20:27:03.63 AS.seek 1ml
@20:27:32.65 delay 1 second
@20:27:33.67 AFV.select :holdingCoil
@20:27:33.77 delay 1 second
@20:27:34.79 ATV.dial :waste
@20:27:36.58 AS.reconfigure ASnorm
@20:27:36.64 AS.seek 0ml
@20:28:27.33 AFV.select :carrierSolution
@20:28:27.45 delay 2 seconds
@20:28:29.47 AS.reconfigure ASconfig
@20:28:29.53 AS.seek 0.5ml
@20:28:58.58 delay 1 second
@20:28:59.61 AFV.select :holdingCoil
@20:28:59.71 Building Slug
@20:28:59.72 ATV.dial :ARV
@20:29:01.48 delay 2 seconds
@20:29:03.50 ARV.dial :air1
@20:29:03.91 delay 2 seconds
@20:29:05.98 AS.reconfigure ASslow2
@20:29:06.03 AS.seek 0.506ml
@20:29:12.53 delay 2 seconds
@20:29:14.56 ARV.dial :MM1
@20:29:15.20 delay 2 seconds
@20:29:17.22 Adding MM1
@20:29:17.30 AS.seek 0.524ml
@20:29:36.01 delay 25 seconds
@20:30:01.03 ARV.dial :PP2
@20:30:01.47 Adding PP2
@20:30:01.48 delay 2 seconds
@20:30:03.56 AS.seek 0.53ml
@20:30:09.92 delay 10 seconds
@20:30:19.96 ARV.dial :air2
@20:30:20.37 adding ARV air
@20:30:20.38 delay 2 seconds
@20:30:22.46 AS.seek 0.536ml
@20:30:28.78 delay 5 seconds
@20:30:33.81 ARV.dial :carrier
@20:30:35.17 delay 7 seconds
@20:30:42.24 AS.seek 0.594ml
@20:31:41.12 delay 5 seconds
@20:31:46.14 ATV.dial :extSrc
@20:31:46.73 delay 2 seconds
@20:31:48.80 AS.seek 0.6ml
@20:31:55.16 delay 4 seconds
@20:31:59.19 ATV.dial ARV
@20:31:59.87 delay 2 seconds
@20:32:01.89 ARV.dial :carrier
@20:32:01.90 delay 2 seconds
@20:32:03.98 AS.seek 0.65ml
@20:32:54.53 delay 4 seconds
@20:32:58.55 ATV.dial PCR
@20:32:59.35 ASV.dial :waste1
@20:32:59.37 ARV.dial :air1
@20:33:00.20 Positioning Slug by 0.324ml
@20:33:00.29 AS.seek 0.326ml
@20:38:27.01 delay 5 seconds
@20:38:32.03 ATV.dial ARV
@20:38:32.86 ASV.dial :PCRlock
@20:38:33.26 delay 5 seconds
@20:38:38.28 Running ACYPP2 Profile60CIQ2 thermocycling
@20:38:38.29 <PCR> PCR.program Profile60CIQ2
@20:38:38.42 Profile60CIQ2 should complete in < 1:47:50
@20:38:38.43 PCR.run 2:19:47.5
@22:07:07.64 PCR.off
@22:07:08.25 <MFBPCR> Running ACYPP2 MeltProfile60 thermocycling
@22:07:08.26 <PCR> PCR.program MeltProfile60
@22:07:08.37 MeltProfile60 should complete in < 11:55
@22:07:08.38 PCR.run 19:53.75
@22:14:45.91 PCR.off
@22:14:46.81 <MFBPCR> PCR for ACYPP2 completed -- closed /var/log/esp/ACYPP221may0720h.pcr
@22:14:46.88 Cleaning PCR
@22:14:46.89 AFV.select :carrierSolution
@22:14:47.01 delay 2 seconds
@22:14:49.03 AS.reconfigure ASconfig
@22:14:49.09 AS.seek 0.5ml
@22:14:59.39 delay 1 second
@22:15:00.42 AFV.select :holdingCoil
@22:15:00.52 ASV.dial :waste1
@22:15:00.92 ATV.dial :air3
@22:15:02.28 AS.reconfigure ASslow1
@22:15:02.34 AS.seek 0.51ml
@22:15:04.62 delay 2 seconds
@22:15:06.65 ATV.dial :bleach
@22:15:07.30 AS.seek 0.81ml
@22:16:07.91 delay 3 seconds
@22:16:10.94 ATV.dial :air3
@22:16:11.53 delay 3 seconds
@22:16:14.59 AS.seek 0.835ml
@22:16:19.90 delay 3 seconds
@22:16:22.93 ATV.dial :bleach
@22:16:24.26 AS.seek 0.82ml
@22:16:27.60 delay 3 seconds
@22:16:30.63 ARV.dial :waste
@22:16:31.28 delay 3 seconds
@22:16:34.30 ATV.dial :ARV
@22:16:35.22 AS.reconfigure ASconfig
@22:16:35.27 AS.seek 0.47ml
@22:16:55.67 AFV.select :carrierSolution
@22:16:55.79 delay 2 seconds
@22:16:57.81 AS.seek 1ml
@22:17:28.57 delay 1 second
@22:17:29.60 AFV.select :holdingCoil
@22:17:29.70 delay 1 second
@22:17:30.72 AS.reconfigure ASfast
@22:17:30.78 AS.seek 0ml
@22:17:58.11 AFV.select :carrierSolution
@22:17:58.23 delay 2 seconds
@22:18:00.25 AS.reconfigure ASconfig
@22:18:00.31 AS.seek 1ml
@22:18:58.06 delay 1 second
@22:18:59.09 AFV.select :holdingCoil
@22:18:59.19 delay 1 second
@22:19:00.21 AS.reconfigure ASfast
@22:19:00.27 AS.seek 0ml
@22:19:27.60 AFV.select :carrierSolution
@22:19:27.72 delay 2 seconds
@22:19:29.74 AS.reconfigure ASconfig
@22:19:29.80 AS.seek 1ml
@22:20:27.55 delay 1 second
@22:20:28.58 AFV.select :holdingCoil
@22:20:28.68 delay 1 second
@22:20:29.70 AS.reconfigure ASfast
@22:20:29.76 AS.seek 0ml
@22:20:57.10 ARV.dial between carrier and air1
@22:20:57.64 ATV.dial :air3
@22:20:58.98 AS.reconfigure ASslow1
@22:20:59.04 AS.seek 0.01ml
@22:21:01.35 delay 2 seconds
@22:21:03.38 ATV.dial :bleach
@22:21:04.41 AS.seek 0.81ml
@22:23:45.67 delay 3 seconds
@22:23:48.69 ATV.dial :air3
@22:23:49.28 delay 3 seconds
@22:23:52.35 AS.seek 0.835ml
@22:23:57.76 delay 3 seconds
@22:24:00.78 ATV.dial :bleach
@22:24:01.42 AS.seek 0.82ml
@22:24:04.81 delay 3 seconds
@22:24:07.83 ATV.dial PCR
@22:24:08.25 AS.reconfigure ASconfig
@22:24:08.30 AS.seek 0ml
@22:24:55.74 PCR.program :bleachProfile
@22:24:55.81 PCR program should complete in < 2:45
@22:24:55.83 PCR.run 10:00
@22:27:51.62 PCR.off
@22:27:51.67 AFV.select :carrierSolution
@22:27:51.78 delay 2 seconds
@22:27:53.80 AS.seek 1ml
@22:28:51.60 delay 1 second
@22:28:52.63 AFV.select :holdingCoil
@22:28:52.73 delay 1 second
@22:28:53.75 AS.reconfigure ASfast
@22:28:53.81 AS.seek 0ml
@22:29:21.15 delay 3 seconds
@22:29:24.17 AFV.select :carrierSolution
@22:29:24.27 delay 2 seconds
@22:29:26.29 AS.reconfigure ASconfig
@22:29:26.35 AS.seek 1ml
@22:30:24.12 delay 1 second
@22:30:25.14 AFV.select :holdingCoil
@22:30:25.24 delay 1 second
@22:30:26.26 AS.reconfigure ASfast
@22:30:26.32 AS.seek 0ml
@22:30:53.67 delay 3 seconds
@22:30:56.69 AFV.select :carrierSolution
@22:30:56.79 delay 2 seconds
@22:30:58.81 AS.reconfigure ASconfig
@22:30:58.87 AS.seek 1ml
@22:31:56.64 delay 1 second
@22:31:57.66 AFV.select :holdingCoil
@22:31:57.76 delay 1 second
@22:31:58.78 AS.reconfigure ASfast
@22:31:58.84 AS.seek 0ml
@22:32:26.18 delay 3 seconds
@22:32:29.20 AFV.select :carrierSolution
@22:32:29.30 delay 2 seconds
@22:32:31.32 AS.reconfigure ASconfig
@22:32:31.39 AS.seek 1ml
@22:33:29.14 delay 1 second
@22:33:30.16 AFV.select :holdingCoil
@22:33:30.26 delay 1 second
@22:33:31.28 AS.reconfigure ASfast
@22:33:31.34 AS.seek 0ml
@22:33:58.67 delay 3 seconds
@22:34:01.69 AFV.select :carrierSolution
@22:34:01.79 delay 2 seconds
@22:34:03.81 AS.reconfigure ASconfig
@22:34:03.88 AS.seek 1ml
@22:35:01.63 delay 1 second
@22:35:02.66 AFV.select :holdingCoil
@22:35:02.76 delay 1 second
@22:35:03.78 AS.reconfigure ASnorm
@22:35:03.83 AS.seek 0ml
@22:35:54.53 <MAIN> AFV.select :carrierSolution
@22:35:54.63 delay 2 seconds
@22:35:56.65 AS.reconfigure ASconfig
@22:35:56.70 AS.seek 0.5ml
@22:36:25.72 delay 1 second
@22:36:26.74 AFV.select :holdingCoil
@22:36:27.14 ATV.dial :air1
@22:36:28.77 AS.reconfigure ASslow2
@22:36:28.83 AS.seek 0.506ml
@22:36:35.23 ATV.dial :extSrc
@22:36:37.02 AS.seek 0.512ml
@22:36:43.32 delay 5 seconds
@22:36:48.34 ATV.dial :air1
@22:36:50.14 AS.reconfigure ASslow1
@22:36:50.19 AS.seek 0.542ml
@22:36:56.57 ATV.dial :waste
@22:36:57.15 AS.reconfigure ASconfig
@22:36:57.21 AS.seek 0ml
@22:37:28.78 <MFBPCR> Setting up PCR for ACYPP2 -- writing /var/log/esp/ACYPP221may0722h.pcr
@22:37:28.80 Building PCR Slug from extSrc with MM1 mix, PP2 primer
@22:37:28.83 Priming with carrier
@22:37:28.84 ATV.dial ARV
@22:37:30.42 ARV.dial :carrier
@22:37:30.77 AS.reconfigure ASnorm
@22:37:30.83 AS.seek 0.5ml
@22:37:56.36 AFV.select :carrierSolution
@22:37:56.49 delay 2 seconds
@22:37:58.51 AS.reconfigure ASconfig
@22:37:58.58 AS.seek 1ml
@22:38:27.61 delay 1 second
@22:38:28.63 AFV.select :holdingCoil
@22:38:28.73 delay 1 second
@22:38:29.75 ATV.dial :waste
@22:38:31.53 AS.reconfigure ASnorm
@22:38:31.60 AS.seek 0ml
@22:39:22.28 AFV.select :carrierSolution
@22:39:22.40 delay 2 seconds
@22:39:24.42 AS.reconfigure ASconfig
@22:39:24.48 AS.seek 0.5ml
@22:39:53.51 delay 1 second
@22:39:54.53 AFV.select :holdingCoil
@22:39:54.63 Building Slug
@22:39:54.64 ATV.dial :ARV
@22:39:56.39 delay 2 seconds
@22:39:58.41 ARV.dial :air1
@22:39:58.83 delay 2 seconds
@22:40:00.90 AS.reconfigure ASslow2
@22:40:00.96 AS.seek 0.506ml
@22:40:07.58 delay 2 seconds
@22:40:09.61 ARV.dial :MM1
@22:40:10.25 delay 2 seconds
@22:40:12.27 Adding MM1
@22:40:12.34 AS.seek 0.524ml
@22:40:31.01 delay 25 seconds
@22:40:56.03 ARV.dial :PP2
@22:40:56.45 Adding PP2
@22:40:56.46 delay 2 seconds
@22:40:58.53 AS.seek 0.53ml
@22:41:04.89 delay 10 seconds
@22:41:14.93 ARV.dial :air2
@22:41:15.36 adding ARV air
@22:41:15.37 delay 2 seconds
@22:41:17.44 AS.seek 0.536ml
@22:41:24.11 delay 5 seconds
@22:41:29.13 ARV.dial :carrier
@22:41:30.48 delay 7 seconds
@22:41:37.55 AS.seek 0.594ml
@22:42:36.40 delay 5 seconds
@22:42:41.42 ATV.dial :extSrc
@22:42:42.03 delay 2 seconds
@22:42:44.10 AS.seek 0.6ml
@22:42:50.71 delay 4 seconds
@22:42:54.74 ATV.dial ARV
@22:42:55.33 delay 2 seconds
@22:42:57.35 ARV.dial :carrier
@22:42:57.36 delay 2 seconds
@22:42:59.44 AS.seek 0.65ml
@22:43:50.18 delay 4 seconds
@22:43:54.20 ATV.dial PCR
@22:43:55.03 ASV.dial :waste1
@22:43:55.04 ARV.dial :air1
@22:43:55.46 Positioning Slug by 0.324ml
@22:43:55.55 AS.seek 0.326ml
@22:49:21.90 delay 5 seconds
@22:49:26.92 ATV.dial ARV
@22:49:27.74 ASV.dial :PCRlock
@22:49:28.15 delay 5 seconds
@22:49:33.17 Running ACYPP2 Profile60CIQ2 thermocycling
@22:49:33.18 <PCR> PCR.program Profile60CIQ2
@22:49:33.30 Profile60CIQ2 should complete in < 1:47:50
@22:49:33.31 PCR.run 2:19:47.5
@00:18:00.77CEST08-May-21 PCR.off
@00:18:01.39 <MFBPCR> Running ACYPP2 MeltProfile60 thermocycling
@00:18:01.41 <PCR> PCR.program MeltProfile60
@00:18:01.50 MeltProfile60 should complete in < 11:55
@00:18:01.52 PCR.run 19:53.75
@00:25:27.04 PCR.off
@00:25:27.94 <MFBPCR> PCR for ACYPP2 completed -- closed /var/log/esp/ACYPP221may0722h.pcr
@00:25:28.01 Cleaning PCR
@00:25:28.03 AFV.select :carrierSolution
@00:25:28.15 delay 2 seconds
@00:25:30.17 AS.reconfigure ASconfig
@00:25:30.23 AS.seek 0.5ml
@00:25:40.54 delay 1 second
@00:25:41.56 AFV.select :holdingCoil
@00:25:41.66 ASV.dial :waste1
@00:25:42.06 ATV.dial :air3
@00:25:43.41 AS.reconfigure ASslow1
@00:25:43.47 AS.seek 0.51ml
@00:25:45.73 delay 2 seconds
@00:25:47.76 ATV.dial :bleach
@00:25:48.39 AS.seek 0.81ml
@00:26:49.00 delay 3 seconds
@00:26:52.02 ATV.dial :air3
@00:26:52.61 delay 3 seconds
@00:26:55.68 AS.seek 0.835ml
@00:27:00.97 delay 3 seconds
@00:27:04.00 ATV.dial :bleach
@00:27:04.66 AS.seek 0.82ml
@00:27:08.11 delay 3 seconds
@00:27:11.13 ARV.dial :waste
@00:27:11.76 delay 3 seconds
@00:27:14.78 ATV.dial :ARV
@00:27:15.70 AS.reconfigure ASconfig
@00:27:15.75 AS.seek 0.47ml
@00:27:36.13 AFV.select :carrierSolution
@00:27:36.25 delay 2 seconds
@00:27:38.27 AS.seek 1ml
@00:28:09.01 delay 1 second
@00:28:10.03 AFV.select :holdingCoil
@00:28:10.13 delay 1 second
@00:28:11.15 AS.reconfigure ASfast
@00:28:11.21 AS.seek 0ml
@00:28:38.55 AFV.select :carrierSolution
@00:28:38.67 delay 2 seconds
@00:28:40.69 AS.reconfigure ASconfig
@00:28:40.74 AS.seek 1ml
@00:29:38.50 delay 1 second
@00:29:39.52 AFV.select :holdingCoil
@00:29:39.62 delay 1 second
@00:29:40.64 AS.reconfigure ASfast
@00:29:40.70 AS.seek 0ml
@00:30:08.02 AFV.select :carrierSolution
@00:30:08.14 delay 2 seconds
@00:30:10.16 AS.reconfigure ASconfig
@00:30:10.22 AS.seek 1ml
@00:31:07.97 delay 1 second
@00:31:09.00 AFV.select :holdingCoil
@00:31:09.10 delay 1 second
@00:31:10.12 AS.reconfigure ASfast
@00:31:10.18 AS.seek 0ml
@00:31:37.52 ARV.dial between carrier and air1
@00:31:38.06 ATV.dial :air3
@00:31:39.41 AS.reconfigure ASslow1
@00:31:39.47 AS.seek 0.01ml
@00:31:41.77 delay 2 seconds
@00:31:43.80 ATV.dial :bleach
@00:31:44.44 AS.seek 0.81ml
@00:34:25.57 delay 3 seconds
@00:34:28.60 ATV.dial :air3
@00:34:29.18 delay 3 seconds
@00:34:32.25 AS.seek 0.835ml
@00:34:37.61 delay 3 seconds
@00:34:40.64 ATV.dial :bleach
@00:34:41.28 AS.seek 0.82ml
@00:34:44.56 delay 3 seconds
@00:34:47.58 ATV.dial PCR
@00:34:48.01 AS.reconfigure ASconfig
@00:34:48.06 AS.seek 0ml
@00:35:35.47 PCR.program :bleachProfile
@00:35:35.54 PCR program should complete in < 2:45
@00:35:35.56 PCR.run 10:00
@00:38:31.73 PCR.off
@00:38:31.77 AFV.select :carrierSolution
@00:38:31.88 delay 2 seconds
@00:38:33.90 AS.seek 1ml
@00:39:31.64 delay 1 second
@00:39:32.66 AFV.select :holdingCoil
@00:39:32.76 delay 1 second
@00:39:33.78 AS.reconfigure ASfast
@00:39:33.84 AS.seek 0ml
@00:40:01.18 delay 3 seconds
@00:40:04.20 AFV.select :carrierSolution
@00:40:04.30 delay 2 seconds
@00:40:06.32 AS.reconfigure ASconfig
@00:40:06.38 AS.seek 1ml
@00:41:04.14 delay 1 second
@00:41:05.16 AFV.select :holdingCoil
@00:41:05.26 delay 1 second
@00:41:06.28 AS.reconfigure ASfast
@00:41:06.34 AS.seek 0ml
@00:41:33.68 delay 3 seconds
@00:41:36.70 AFV.select :carrierSolution
@00:41:36.80 delay 2 seconds
@00:41:38.82 AS.reconfigure ASconfig
@00:41:38.88 AS.seek 1ml
@00:42:36.64 delay 1 second
@00:42:37.66 AFV.select :holdingCoil
@00:42:37.76 delay 1 second
@00:42:38.78 AS.reconfigure ASfast
@00:42:38.84 AS.seek 0ml
@00:43:06.18 delay 3 seconds
@00:43:09.20 AFV.select :carrierSolution
@00:43:09.30 delay 2 seconds
@00:43:11.32 AS.reconfigure ASconfig
@00:43:11.38 AS.seek 1ml
@00:44:09.14 delay 1 second
@00:44:10.16 AFV.select :holdingCoil
@00:44:10.26 delay 1 second
@00:44:11.28 AS.reconfigure ASfast
@00:44:11.34 AS.seek 0ml
@00:44:38.68 delay 3 seconds
@00:44:41.70 AFV.select :carrierSolution
@00:44:41.80 delay 2 seconds
@00:44:43.82 AS.reconfigure ASconfig
@00:44:43.88 AS.seek 1ml
@00:45:41.65 delay 1 second
@00:45:42.67 AFV.select :holdingCoil
@00:45:42.77 delay 1 second
@00:45:43.79 AS.reconfigure ASnorm
@00:45:43.84 AS.seek 0ml
@00:46:34.54 <MAIN> AFV.select :carrierSolution
@00:46:34.65 delay 2 seconds
@00:46:36.67 AS.reconfigure ASconfig
@00:46:36.72 AS.seek 0.5ml
@00:47:05.77 delay 1 second
@00:47:06.80 AFV.select :holdingCoil
@00:47:06.90 ATV.dial :air1
@00:47:08.47 AS.reconfigure ASslow1
@00:47:08.53 AS.seek 0.52ml
@00:47:12.87 delay 5 seconds
@00:47:17.89 ATV.dial :extSrc
@00:47:19.67 AS.reconfigure ASslow2
@00:47:19.72 AS.seek 0.545ml
@00:47:45.22 delay 10 seconds
@00:47:55.24 ATV.dial :air1
@00:47:57.03 AS.reconfigure ASslow1
@00:47:57.08 AS.seek 0.575ml
@00:48:03.48 ATV.dial :waste
@00:48:04.08 AS.reconfigure ASconfig
@00:48:04.13 AS.seek 0ml
@00:48:37.61 <MFBPCR> Setting up PCR for ACYPP2 -- writing /var/log/esp/ACYPP221may0800h.pcr
@00:48:37.63 Building PCR Slug from extSrc with MM1 mix, PP2 primer
@00:48:37.66 Priming with carrier
@00:48:37.68 ATV.dial ARV
@00:48:39.26 ARV.dial :carrier
@00:48:39.59 AS.reconfigure ASnorm
@00:48:39.66 AS.seek 0.5ml
@00:49:05.19 AFV.select :carrierSolution
@00:49:05.32 delay 2 seconds
@00:49:07.34 AS.reconfigure ASconfig
@00:49:07.41 AS.seek 1ml
@00:49:36.43 delay 1 second
@00:49:37.45 AFV.select :holdingCoil
@00:49:37.55 delay 1 second
@00:49:38.57 ATV.dial :waste
@00:49:40.34 AS.reconfigure ASnorm
@00:49:40.40 AS.seek 0ml
@00:50:31.10 AFV.select :carrierSolution
@00:50:31.22 delay 2 seconds
@00:50:33.24 AS.reconfigure ASconfig
@00:50:33.30 AS.seek 0.5ml
@00:51:02.38 delay 1 second
@00:51:03.41 AFV.select :holdingCoil
@00:51:03.51 Building Slug
@00:51:03.52 ATV.dial :ARV
@00:51:05.28 delay 2 seconds
@00:51:07.66 ARV.dial :air1
@00:51:08.09 delay 2 seconds
@00:51:10.16 AS.reconfigure ASslow2
@00:51:10.22 AS.seek 0.506ml
@00:51:16.75 delay 2 seconds
@00:51:18.77 ARV.dial :MM1
@00:51:19.40 delay 2 seconds
@00:51:21.42 Adding MM1
@00:51:21.50 AS.seek 0.524ml
@00:51:40.09 delay 25 seconds
@00:52:05.11 ARV.dial :PP2
@00:52:05.53 Adding PP2
@00:52:05.54 delay 2 seconds
@00:52:07.62 AS.seek 0.53ml
@00:52:13.97 delay 10 seconds
@00:52:24.00 ARV.dial :air2
@00:52:24.42 adding ARV air
@00:52:24.43 delay 2 seconds
@00:52:26.51 AS.seek 0.536ml
@00:52:33.03 delay 5 seconds
@00:52:38.05 ARV.dial :carrier
@00:52:39.40 delay 7 seconds
@00:52:46.47 AS.seek 0.594ml
@00:53:45.08 delay 5 seconds
@00:53:50.10 ATV.dial :extSrc
@00:53:50.70 delay 2 seconds
@00:53:52.77 AS.seek 0.6ml
@00:53:59.17 delay 4 seconds
@00:54:03.19 ATV.dial ARV
@00:54:03.78 delay 2 seconds
@00:54:05.80 ARV.dial :carrier
@00:54:05.81 delay 2 seconds
@00:54:07.89 AS.seek 0.65ml
@00:54:58.68 delay 4 seconds
@00:55:02.70 ATV.dial PCR
@00:55:03.53 ASV.dial :waste1
@00:55:03.54 ARV.dial :air1
@00:55:03.96 Positioning Slug by 0.324ml
@00:55:04.05 AS.seek 0.326ml
@01:00:30.34 delay 5 seconds
@01:00:35.36 ATV.dial ARV
@01:00:36.18 ASV.dial :PCRlock
@01:00:36.58 delay 5 seconds
@01:00:41.60 Running ACYPP2 Profile60CIQ2 thermocycling
@01:00:41.61 <PCR> PCR.program Profile60CIQ2
@01:00:41.73 Profile60CIQ2 should complete in < 1:47:50
@01:00:41.74 PCR.run 2:19:47.5
@02:29:04.90 PCR.off
@02:29:05.50 <MFBPCR> Running ACYPP2 MeltProfile60 thermocycling
@02:29:05.52 <PCR> PCR.program MeltProfile60
@02:29:05.62 MeltProfile60 should complete in < 11:55
@02:29:05.63 PCR.run 19:53.75
@02:36:43.55 PCR.off
@02:36:44.64 <MFBPCR> PCR for ACYPP2 completed -- closed /var/log/esp/ACYPP221may0800h.pcr
@02:36:44.77 Cleaning PCR
@02:36:44.79 AFV.select :carrierSolution
@02:36:44.96 delay 2 seconds
@02:36:46.99 AS.reconfigure ASconfig
@02:36:47.10 AS.seek 0.5ml
@02:36:57.41 delay 1 second
@02:36:58.44 AFV.select :holdingCoil
@02:36:58.56 ASV.dial :waste1
@02:36:58.97 ATV.dial :air3
@02:37:00.31 AS.reconfigure ASslow1
@02:37:00.37 AS.seek 0.51ml
@02:37:02.66 delay 2 seconds
@02:37:04.69 ATV.dial :bleach
@02:37:05.33 AS.seek 0.81ml
@02:38:05.89 delay 3 seconds
@02:38:08.92 ATV.dial :air3
@02:38:09.51 delay 3 seconds
@02:38:12.58 AS.seek 0.835ml
@02:38:17.89 delay 3 seconds
@02:38:21.26 ATV.dial :bleach
@02:38:21.92 AS.seek 0.82ml
@02:38:25.25 delay 3 seconds
@02:38:28.27 ARV.dial :waste
@02:38:28.90 delay 3 seconds
@02:38:31.92 ATV.dial :ARV
@02:38:32.84 AS.reconfigure ASconfig
@02:38:32.90 AS.seek 0.47ml
@02:38:53.28 AFV.select :carrierSolution
@02:38:53.40 delay 2 seconds
@02:38:55.42 AS.seek 1ml
@02:39:26.17 delay 1 second
@02:39:27.19 AFV.select :holdingCoil
@02:39:27.29 delay 1 second
@02:39:28.31 AS.reconfigure ASfast
@02:39:28.37 AS.seek 0ml
@02:39:55.69 AFV.select :carrierSolution
@02:39:55.81 delay 2 seconds
@02:39:57.83 AS.reconfigure ASconfig
@02:39:57.89 AS.seek 1ml
@02:40:55.64 delay 1 second
@02:40:56.66 AFV.select :holdingCoil
@02:40:56.76 delay 1 second
@02:40:57.78 AS.reconfigure ASfast
@02:40:57.84 AS.seek 0ml
@02:41:25.18 AFV.select :carrierSolution
@02:41:25.30 delay 2 seconds
@02:41:27.32 AS.reconfigure ASconfig
@02:41:27.38 AS.seek 1ml
@02:42:25.12 delay 1 second
@02:42:26.14 AFV.select :holdingCoil
@02:42:26.24 delay 1 second
@02:42:27.26 AS.reconfigure ASfast
@02:42:27.32 AS.seek 0ml
@02:42:54.66 ARV.dial between carrier and air1
@02:42:55.20 ATV.dial :air3
@02:42:56.55 AS.reconfigure ASslow1
@02:42:56.61 AS.seek 0.01ml
@02:42:59.02 delay 2 seconds
@02:43:01.05 ATV.dial :bleach
@02:43:01.70 AS.seek 0.81ml
@02:45:43.00 delay 3 seconds
@02:45:46.02 ATV.dial :air3
@02:45:46.61 delay 3 seconds
@02:45:49.68 AS.seek 0.835ml
@02:45:55.07 delay 3 seconds
@02:45:58.10 ATV.dial :bleach
@02:45:58.74 AS.seek 0.82ml
@02:46:02.16 delay 3 seconds
@02:46:05.19 ATV.dial PCR
@02:46:05.61 AS.reconfigure ASconfig
@02:46:05.66 AS.seek 0ml
@02:46:53.10 PCR.program :bleachProfile
@02:46:53.17 PCR program should complete in < 2:45
@02:46:53.19 PCR.run 10:00
@02:49:48.98 PCR.off
@02:49:49.03 AFV.select :carrierSolution
@02:49:49.14 delay 2 seconds
@02:49:51.16 AS.seek 1ml
@02:50:49.34 delay 1 second
@02:50:50.36 AFV.select :holdingCoil
@02:50:50.46 delay 1 second
@02:50:51.48 AS.reconfigure ASfast
@02:50:51.54 AS.seek 0ml
@02:51:18.87 delay 3 seconds
@02:51:21.89 AFV.select :carrierSolution
@02:51:21.99 delay 2 seconds
@02:51:24.01 AS.reconfigure ASconfig
@02:51:24.07 AS.seek 1ml
@02:52:21.83 delay 1 second
@02:52:22.85 AFV.select :holdingCoil
@02:52:22.95 delay 1 second
@02:52:23.97 AS.reconfigure ASfast
@02:52:24.03 AS.seek 0ml
@02:52:51.37 delay 3 seconds
@02:52:54.39 AFV.select :carrierSolution
@02:52:54.49 delay 2 seconds
@02:52:56.51 AS.reconfigure ASconfig
@02:52:56.57 AS.seek 1ml
@02:53:54.34 delay 1 second
@02:53:55.36 AFV.select :holdingCoil
@02:53:55.46 delay 1 second
@02:53:56.48 AS.reconfigure ASfast
@02:53:56.54 AS.seek 0ml
@02:54:23.89 delay 3 seconds
@02:54:26.91 AFV.select :carrierSolution
@02:54:27.01 delay 2 seconds
@02:54:29.03 AS.reconfigure ASconfig
@02:54:29.09 AS.seek 1ml
@02:55:26.84 delay 1 second
@02:55:27.86 AFV.select :holdingCoil
@02:55:27.96 delay 1 second
@02:55:28.98 AS.reconfigure ASfast
@02:55:29.04 AS.seek 0ml
@02:55:56.37 delay 3 seconds
@02:55:59.39 AFV.select :carrierSolution
@02:55:59.49 delay 2 seconds
@02:56:01.51 AS.reconfigure ASconfig
@02:56:01.57 AS.seek 1ml
@02:56:59.33 delay 1 second
@02:57:00.35 AFV.select :holdingCoil
@02:57:00.45 delay 1 second
@02:57:01.47 AS.reconfigure ASnorm
@02:57:01.52 AS.seek 0ml
@02:57:52.22 <MAIN> AFV.select :carrierSolution
@02:57:52.33 delay 2 seconds
@02:57:54.35 AS.reconfigure ASconfig
@02:57:54.41 AS.seek 0.5ml
@02:58:23.47 delay 1 second
@02:58:24.50 AFV.select :holdingCoil
@02:58:24.60 ATV.dial :air1
@02:58:26.17 AS.reconfigure ASslow2
@02:58:26.22 AS.seek 0.506ml
@02:58:32.67 ATV.dial :extSrc
@02:58:34.45 AS.seek 0.512ml
@02:58:40.69 delay 5 seconds
@02:58:45.71 ATV.dial :air1
@02:58:47.52 AS.reconfigure ASslow1
@02:58:47.58 AS.seek 0.542ml
@02:58:53.89 ATV.dial :waste
@02:58:54.47 AS.reconfigure ASconfig
@02:58:54.53 AS.seek 0ml
@02:59:26.12 <MFBPCR> Setting up PCR for ACYPP2 -- writing /var/log/esp/ACYPP221may0802h.pcr
@02:59:26.14 Building PCR Slug from extSrc with MM1 mix, PP2 primer
@02:59:26.17 Priming with carrier
@02:59:26.18 ATV.dial ARV
@02:59:27.76 ARV.dial :carrier
@02:59:28.09 AS.reconfigure ASnorm
@02:59:28.16 AS.seek 0.5ml
@02:59:53.68 AFV.select :carrierSolution
@02:59:53.81 delay 2 seconds
@02:59:55.83 AS.reconfigure ASconfig
@02:59:55.89 AS.seek 1ml
@03:00:24.95 delay 1 second
@03:00:25.97 AFV.select :holdingCoil
@03:00:26.07 delay 1 second
@03:00:27.09 ATV.dial :waste
@03:00:28.87 AS.reconfigure ASnorm
@03:00:28.93 AS.seek 0ml
@03:01:19.61 AFV.select :carrierSolution
@03:01:19.73 delay 2 seconds
@03:01:21.75 AS.reconfigure ASconfig
@03:01:21.81 AS.seek 0.5ml
@03:01:50.88 delay 1 second
@03:01:51.91 AFV.select :holdingCoil
@03:01:52.01 Building Slug
@03:01:52.02 ATV.dial :ARV
@03:01:53.78 delay 2 seconds
@03:01:55.80 ARV.dial :air1
@03:01:56.20 delay 2 seconds
@03:01:58.26 AS.reconfigure ASslow2
@03:01:58.32 AS.seek 0.506ml
@03:02:04.59 delay 2 seconds
@03:02:06.61 ARV.dial :MM1
@03:02:07.25 delay 2 seconds
@03:02:09.27 Adding MM1
@03:02:09.34 AS.seek 0.524ml
@03:02:27.90 delay 25 seconds
@03:02:52.92 ARV.dial :PP2
@03:02:53.34 Adding PP2
@03:02:53.35 delay 2 seconds
@03:02:55.43 AS.seek 0.53ml
@03:03:01.79 delay 10 seconds
@03:03:11.82 ARV.dial :air2
@03:03:12.25 adding ARV air
@03:03:12.26 delay 2 seconds
@03:03:14.34 AS.seek 0.536ml
@03:03:20.93 delay 5 seconds
@03:03:25.96 ARV.dial :carrier
@03:03:27.31 delay 7 seconds
@03:03:34.39 AS.seek 0.594ml
@03:04:32.90 delay 5 seconds
@03:04:37.92 ATV.dial :extSrc
@03:04:38.53 delay 2 seconds
@03:04:40.60 AS.seek 0.6ml
@03:04:47.29 delay 4 seconds
@03:04:51.31 ATV.dial ARV
@03:04:51.89 delay 2 seconds
@03:04:53.91 ARV.dial :carrier
@03:04:53.92 delay 2 seconds
@03:04:56.00 AS.seek 0.65ml
@03:05:46.89 delay 4 seconds
@03:05:50.91 ATV.dial PCR
@03:05:52.11 ASV.dial :waste1
@03:05:52.12 ARV.dial :air1
@03:05:52.54 Positioning Slug by 0.324ml
@03:05:52.63 AS.seek 0.326ml
@03:11:19.19 delay 5 seconds
@03:11:24.21 ATV.dial ARV
@03:11:25.03 ASV.dial :PCRlock
@03:11:25.44 delay 5 seconds
@03:11:30.46 Running ACYPP2 Profile60CIQ2 thermocycling
@03:11:30.47 <PCR> PCR.program Profile60CIQ2
@03:11:30.60 Profile60CIQ2 should complete in < 1:47:50
@03:11:30.61 PCR.run 2:19:47.5
@04:39:50.15 PCR.off
@04:39:50.76 <MFBPCR> Running ACYPP2 MeltProfile60 thermocycling
@04:39:50.77 <PCR> PCR.program MeltProfile60
@04:39:50.88 MeltProfile60 should complete in < 11:55
@04:39:50.89 PCR.run 19:53.75
@04:47:19.76 PCR.off
@04:47:20.66 <MFBPCR> PCR for ACYPP2 completed -- closed /var/log/esp/ACYPP221may0802h.pcr
@04:47:20.73 Cleaning PCR
@04:47:20.74 AFV.select :carrierSolution
@04:47:20.86 delay 2 seconds
@04:47:22.88 AS.reconfigure ASconfig
@04:47:22.94 AS.seek 0.5ml
@04:47:33.22 delay 1 second
@04:47:34.25 AFV.select :holdingCoil
@04:47:34.35 ASV.dial :waste1
@04:47:34.75 ATV.dial :air3
@04:47:36.10 AS.reconfigure ASslow1
@04:47:36.16 AS.seek 0.51ml
@04:47:38.44 delay 2 seconds
@04:47:40.47 ATV.dial :bleach
@04:47:41.11 AS.seek 0.81ml
@04:48:41.69 delay 3 seconds
@04:48:44.72 ATV.dial :air3
@04:48:45.31 delay 3 seconds
@04:48:48.38 AS.seek 0.835ml
@04:48:53.65 delay 3 seconds
@04:48:56.68 ATV.dial :bleach
@04:48:57.31 AS.seek 0.82ml
@04:49:00.61 delay 3 seconds
@04:49:03.64 ARV.dial :waste
@04:49:04.30 delay 3 seconds
@04:49:07.32 ATV.dial :ARV
@04:49:08.25 AS.reconfigure ASconfig
@04:49:08.30 AS.seek 0.47ml
@04:49:28.72 AFV.select :carrierSolution
@04:49:28.84 delay 2 seconds
@04:49:30.86 AS.seek 1ml
@04:50:01.61 delay 1 second
@04:50:02.64 AFV.select :holdingCoil
@04:50:02.74 delay 1 second
@04:50:03.76 AS.reconfigure ASfast
@04:50:03.82 AS.seek 0ml
@04:50:31.14 AFV.select :carrierSolution
@04:50:31.26 delay 2 seconds
@04:50:33.28 AS.reconfigure ASconfig
@04:50:33.34 AS.seek 1ml
@04:51:31.09 delay 1 second
@04:51:32.11 AFV.select :holdingCoil
@04:51:32.21 delay 1 second
@04:51:33.23 AS.reconfigure ASfast
@04:51:33.28 AS.seek 0ml
@04:52:00.60 AFV.select :carrierSolution
@04:52:00.72 delay 2 seconds
@04:52:02.74 AS.reconfigure ASconfig
@04:52:02.80 AS.seek 1ml
@04:53:00.55 delay 1 second
@04:53:01.58 AFV.select :holdingCoil
@04:53:01.68 delay 1 second
@04:53:02.70 AS.reconfigure ASfast
@04:53:02.76 AS.seek 0ml
@04:53:30.10 ARV.dial between carrier and air1
@04:53:30.64 ATV.dial :air3
@04:53:32.00 AS.reconfigure ASslow1
@04:53:32.06 AS.seek 0.01ml
@04:53:34.36 delay 2 seconds
@04:53:36.39 ATV.dial :bleach
@04:53:37.05 AS.seek 0.81ml
@04:56:18.19 delay 3 seconds
@04:56:21.22 ATV.dial :air3
@04:56:22.20 delay 3 seconds
@04:56:25.28 AS.seek 0.835ml
@04:56:30.56 delay 3 seconds
@04:56:33.59 ATV.dial :bleach
@04:56:34.24 AS.seek 0.82ml
@04:56:37.67 delay 3 seconds
@04:56:40.69 ATV.dial PCR
@04:56:41.12 AS.reconfigure ASconfig
@04:56:41.17 AS.seek 0ml
@04:57:28.55 PCR.program :bleachProfile
@04:57:28.63 PCR program should complete in < 2:45
@04:57:28.64 PCR.run 10:00
@05:00:24.43 PCR.off
@05:00:24.47 AFV.select :carrierSolution
@05:00:24.58 delay 2 seconds
@05:00:26.60 AS.seek 1ml
@05:01:24.41 delay 1 second
@05:01:25.44 AFV.select :holdingCoil
@05:01:25.54 delay 1 second
@05:01:26.56 AS.reconfigure ASfast
@05:01:26.62 AS.seek 0ml
@05:01:53.97 delay 3 seconds
@05:01:56.99 AFV.select :carrierSolution
@05:01:57.09 delay 2 seconds
@05:01:59.11 AS.reconfigure ASconfig
@05:01:59.18 AS.seek 1ml
@05:02:56.93 delay 1 second
@05:02:57.96 AFV.select :holdingCoil
@05:02:58.06 delay 1 second
@05:02:59.08 AS.reconfigure ASfast
@05:02:59.14 AS.seek 0ml
@05:03:26.48 delay 3 seconds
@05:03:29.50 AFV.select :carrierSolution
@05:03:29.60 delay 2 seconds
@05:03:31.62 AS.reconfigure ASconfig
@05:03:31.69 AS.seek 1ml
@05:04:29.44 delay 1 second
@05:04:30.47 AFV.select :holdingCoil
@05:04:30.57 delay 1 second
@05:04:31.59 AS.reconfigure ASfast
@05:04:31.65 AS.seek 0ml
@05:04:58.98 delay 3 seconds
@05:05:02.00 AFV.select :carrierSolution
@05:05:02.10 delay 2 seconds
@05:05:04.12 AS.reconfigure ASconfig
@05:05:04.19 AS.seek 1ml
@05:06:01.93 delay 1 second
@05:06:02.96 AFV.select :holdingCoil
@05:06:03.06 delay 1 second
@05:06:04.08 AS.reconfigure ASfast
@05:06:04.14 AS.seek 0ml
@05:06:31.48 delay 3 seconds
@05:06:34.50 AFV.select :carrierSolution
@05:06:34.60 delay 2 seconds
@05:06:36.62 AS.reconfigure ASconfig
@05:06:36.69 AS.seek 1ml
@05:07:34.49 delay 1 second
@05:07:35.52 AFV.select :holdingCoil
@05:07:35.62 delay 1 second
@05:07:36.64 AS.reconfigure ASnorm
@05:07:36.69 AS.seek 0ml
@05:08:27.41 <MAIN> AFV.select :carrierSolution
@05:08:27.51 delay 2 seconds
@05:08:29.53 AS.reconfigure ASconfig
@05:08:29.58 AS.seek 0.5ml
@05:08:58.63 delay 1 second
@05:08:59.65 AFV.select :holdingCoil
@05:08:59.75 ATV.dial :air1
@05:09:01.33 AS.reconfigure ASslow1
@05:09:01.39 AS.seek 0.52ml
@05:09:06.14 delay 5 seconds
@05:09:11.16 ATV.dial :extSrc
@05:09:12.95 AS.reconfigure ASslow2
@05:09:13.00 AS.seek 0.538ml
@05:09:31.55 delay 10 seconds
@05:09:41.57 ATV.dial :air1
@05:09:43.38 AS.reconfigure ASslow1
@05:09:43.43 AS.seek 0.568ml
@05:09:49.89 ATV.dial :waste
@05:09:50.47 AS.reconfigure ASconfig
@05:09:50.53 AS.seek 0ml
@05:10:23.60 <MFBPCR> Setting up PCR for ACYPP2 -- writing /var/log/esp/ACYPP221may0805h.pcr
@05:10:23.62 Building PCR Slug from extSrc with MM1 mix, PP2 primer
@05:10:23.65 Priming with carrier
@05:10:23.67 ATV.dial ARV
@05:10:25.26 ARV.dial :carrier
@05:10:25.61 AS.reconfigure ASnorm
@05:10:25.68 AS.seek 0.5ml
@05:10:51.27 AFV.select :carrierSolution
@05:10:51.40 delay 2 seconds
@05:10:53.42 AS.reconfigure ASconfig
@05:10:53.49 AS.seek 1ml
@05:11:22.53 delay 1 second
@05:11:23.55 AFV.select :holdingCoil
@05:11:23.65 delay 1 second
@05:11:24.67 ATV.dial :waste
@05:11:26.45 AS.reconfigure ASnorm
@05:11:26.51 AS.seek 0ml
@05:12:17.22 AFV.select :carrierSolution
@05:12:17.35 delay 2 seconds
@05:12:19.37 AS.reconfigure ASconfig
@05:12:19.43 AS.seek 0.5ml
@05:12:48.48 delay 1 second
@05:12:49.50 AFV.select :holdingCoil
@05:12:49.60 Building Slug
@05:12:49.61 ATV.dial :ARV
@05:12:51.37 delay 2 seconds
@05:12:53.39 ARV.dial :air1
@05:12:53.84 delay 2 seconds
@05:12:55.92 AS.reconfigure ASslow2
@05:12:55.98 AS.seek 0.506ml
@05:13:02.59 delay 2 seconds
@05:13:04.61 ARV.dial :MM1
@05:13:05.25 delay 2 seconds
@05:13:07.27 Adding MM1
@05:13:07.35 AS.seek 0.524ml
@05:13:25.76 delay 25 seconds
@05:13:50.78 ARV.dial :PP2
@05:13:51.20 Adding PP2
@05:13:51.21 delay 2 seconds
@05:13:53.29 AS.seek 0.53ml
@05:13:59.51 delay 10 seconds
@05:14:09.54 ARV.dial :air2
@05:14:09.95 adding ARV air
@05:14:09.96 delay 2 seconds
@05:14:12.04 AS.seek 0.536ml
@05:14:18.50 delay 5 seconds
@05:14:23.52 ARV.dial :carrier
@05:14:24.87 delay 7 seconds
@05:14:31.95 AS.seek 0.594ml
@05:15:30.98 delay 5 seconds
@05:15:36.00 ATV.dial :extSrc
@05:15:36.59 delay 2 seconds
@05:15:38.66 AS.seek 0.6ml
@05:15:45.37 delay 4 seconds
@05:15:49.39 ATV.dial ARV
@05:15:50.08 delay 2 seconds
@05:15:52.10 ARV.dial :carrier
@05:15:52.11 delay 2 seconds
@05:15:54.19 AS.seek 0.65ml
@05:16:45.22 delay 4 seconds
@05:16:49.24 ATV.dial PCR
@05:16:50.06 ASV.dial :waste1
@05:16:50.07 ARV.dial :air1
@05:16:50.51 Positioning Slug by 0.324ml
@05:16:50.59 AS.seek 0.326ml
@05:22:16.92 delay 5 seconds
@05:22:21.94 ATV.dial ARV
@05:22:22.78 ASV.dial :PCRlock
@05:22:23.19 delay 5 seconds
@05:22:28.21 Running ACYPP2 Profile60CIQ2 thermocycling
@05:22:28.22 <PCR> PCR.program Profile60CIQ2
@05:22:28.34 Profile60CIQ2 should complete in < 1:47:50
@05:22:28.35 PCR.run 2:19:47.5
@06:50:47.70 PCR.off
@06:50:48.30 <MFBPCR> Running ACYPP2 MeltProfile60 thermocycling
@06:50:48.32 <PCR> PCR.program MeltProfile60
@06:50:48.41 MeltProfile60 should complete in < 11:55
@06:50:48.42 PCR.run 19:53.75
@06:58:17.38 PCR.off
@06:58:18.28 <MFBPCR> PCR for ACYPP2 completed -- closed /var/log/esp/ACYPP221may0805h.pcr
@06:58:18.35 Cleaning PCR
@06:58:18.37 AFV.select :carrierSolution
@06:58:18.49 delay 2 seconds
@06:58:20.51 AS.reconfigure ASconfig
@06:58:20.57 AS.seek 0.5ml
@06:58:30.89 delay 1 second
@06:58:31.91 AFV.select :holdingCoil
@06:58:32.01 ASV.dial :waste1
@06:58:32.41 ATV.dial :air3
@06:58:33.75 AS.reconfigure ASslow1
@06:58:33.81 AS.seek 0.51ml
@06:58:36.11 delay 2 seconds
@06:58:38.13 ATV.dial :bleach
@06:58:38.78 AS.seek 0.81ml
@06:59:39.36 delay 3 seconds
@06:59:42.38 ATV.dial :air3
@06:59:42.98 delay 3 seconds
@06:59:46.05 AS.seek 0.835ml
@06:59:51.45 delay 3 seconds
@06:59:54.47 ATV.dial :bleach
@06:59:55.13 AS.seek 0.82ml
@06:59:58.44 delay 3 seconds
@07:00:01.46 ARV.dial :waste
@07:00:02.11 delay 3 seconds
@07:00:05.13 ATV.dial :ARV
@07:00:06.05 AS.reconfigure ASconfig
@07:00:06.11 AS.seek 0.47ml
@07:00:26.50 AFV.select :carrierSolution
@07:00:26.62 delay 2 seconds
@07:00:28.64 AS.seek 1ml
@07:00:59.37 delay 1 second
@07:01:00.39 AFV.select :holdingCoil
@07:01:00.49 delay 1 second
@07:01:01.51 AS.reconfigure ASfast
@07:01:01.57 AS.seek 0ml
@07:01:28.91 AFV.select :carrierSolution
@07:01:29.03 delay 2 seconds
@07:01:31.05 AS.reconfigure ASconfig
@07:01:31.10 AS.seek 1ml
@07:02:28.84 delay 1 second
@07:02:29.86 AFV.select :holdingCoil
@07:02:29.96 delay 1 second
@07:02:30.98 AS.reconfigure ASfast
@07:02:31.04 AS.seek 0ml
@07:02:58.35 AFV.select :carrierSolution
@07:02:58.47 delay 2 seconds
@07:03:00.49 AS.reconfigure ASconfig
@07:03:00.54 AS.seek 1ml
@07:03:58.31 delay 1 second
@07:03:59.33 AFV.select :holdingCoil
@07:03:59.43 delay 1 second
@07:04:00.45 AS.reconfigure ASfast
@07:04:00.51 AS.seek 0ml
@07:04:27.80 ARV.dial between carrier and air1
@07:04:28.33 ATV.dial :air3
@07:04:29.67 AS.reconfigure ASslow1
@07:04:29.73 AS.seek 0.01ml
@07:04:32.03 delay 2 seconds
@07:04:34.05 ATV.dial :bleach
@07:04:34.69 AS.seek 0.81ml
@07:07:15.93 delay 3 seconds
@07:07:18.96 ATV.dial :air3
@07:07:19.55 delay 3 seconds
@07:07:22.61 AS.seek 0.835ml
@07:07:27.96 delay 3 seconds
@07:07:30.98 ATV.dial :bleach
@07:07:31.61 AS.seek 0.82ml
@07:07:34.99 delay 3 seconds
@07:07:38.02 ATV.dial PCR
@07:07:38.45 AS.reconfigure ASconfig
@07:07:38.51 AS.seek 0ml
@07:08:25.91 PCR.program :bleachProfile
@07:08:25.98 PCR program should complete in < 2:45
@07:08:26.00 PCR.run 10:00
@07:11:22.15 PCR.off
@07:11:22.19 AFV.select :carrierSolution
@07:11:22.30 delay 2 seconds
@07:11:24.32 AS.seek 1ml
@07:12:22.09 delay 1 second
@07:12:23.11 AFV.select :holdingCoil
@07:12:23.21 delay 1 second
@07:12:24.23 AS.reconfigure ASfast
@07:12:24.29 AS.seek 0ml
@07:12:51.64 delay 3 seconds
@07:12:54.66 AFV.select :carrierSolution
@07:12:54.76 delay 2 seconds
@07:12:56.78 AS.reconfigure ASconfig
@07:12:56.85 AS.seek 1ml
@07:13:54.61 delay 1 second
@07:13:55.63 AFV.select :holdingCoil
@07:13:55.73 delay 1 second
@07:13:56.75 AS.reconfigure ASfast
@07:13:56.81 AS.seek 0ml
@07:14:24.16 delay 3 seconds
@07:14:27.18 AFV.select :carrierSolution
@07:14:27.28 delay 2 seconds
@07:14:29.30 AS.reconfigure ASconfig
@07:14:29.37 AS.seek 1ml
@07:15:27.12 delay 1 second
@07:15:28.14 AFV.select :holdingCoil
@07:15:28.24 delay 1 second
@07:15:29.26 AS.reconfigure ASfast
@07:15:29.32 AS.seek 0ml
@07:15:56.67 delay 3 seconds
@07:15:59.69 AFV.select :carrierSolution
@07:15:59.79 delay 2 seconds
@07:16:01.81 AS.reconfigure ASconfig
@07:16:01.88 AS.seek 1ml
@07:16:59.64 delay 1 second
@07:17:00.66 AFV.select :holdingCoil
@07:17:00.76 delay 1 second
@07:17:01.78 AS.reconfigure ASfast
@07:17:01.84 AS.seek 0ml
@07:17:29.19 delay 3 seconds
@07:17:32.21 AFV.select :carrierSolution
@07:17:32.31 delay 2 seconds
@07:17:34.33 AS.reconfigure ASconfig
@07:17:34.40 AS.seek 1ml
@07:18:32.16 delay 1 second
@07:18:33.19 AFV.select :holdingCoil
@07:18:33.29 delay 1 second
@07:18:34.31 AS.reconfigure ASnorm
@07:18:34.36 AS.seek 0ml
@07:19:25.10 <MAIN> AFV.select :carrierSolution
@07:19:25.20 delay 2 seconds
@07:19:27.22 AS.reconfigure ASconfig
@07:19:27.27 AS.seek 0.5ml
@07:19:56.30 delay 1 second
@07:19:57.33 AFV.select :holdingCoil
@07:19:57.43 ATV.dial :air1
@07:19:59.00 AS.reconfigure ASslow2
@07:19:59.06 AS.seek 0.506ml
@07:20:05.89 ATV.dial :extSrc
@07:20:07.69 AS.seek 0.512ml
@07:20:14.19 delay 5 seconds
@07:20:19.21 ATV.dial :air1
@07:20:21.03 AS.reconfigure ASslow1
@07:20:21.08 AS.seek 0.542ml
@07:20:27.50 ATV.dial :waste
@07:20:28.09 AS.reconfigure ASconfig
@07:20:28.14 AS.seek 0ml
@07:20:59.75 <MFBPCR> Setting up PCR for ACYPP2 -- writing /var/log/esp/ACYPP221may0807h.pcr
@07:20:59.77 Building PCR Slug from extSrc with MM1 mix, PP2 primer
@07:20:59.80 Priming with carrier
@07:20:59.82 ATV.dial ARV
@07:21:01.41 ARV.dial :carrier
@07:21:01.75 AS.reconfigure ASnorm
@07:21:01.82 AS.seek 0.5ml
@07:21:27.33 AFV.select :carrierSolution
@07:21:27.46 delay 2 seconds
@07:21:29.48 AS.reconfigure ASconfig
@07:21:29.55 AS.seek 1ml
@07:21:58.56 delay 1 second
@07:21:59.58 AFV.select :holdingCoil
@07:21:59.68 delay 1 second
@07:22:00.70 ATV.dial :waste
@07:22:02.48 AS.reconfigure ASnorm
@07:22:02.54 AS.seek 0ml
@07:22:53.22 AFV.select :carrierSolution
@07:22:53.34 delay 2 seconds
@07:22:55.36 AS.reconfigure ASconfig
@07:22:55.42 AS.seek 0.5ml
@07:23:24.44 delay 1 second
@07:23:25.47 AFV.select :holdingCoil
@07:23:25.57 Building Slug
@07:23:25.59 ATV.dial :ARV
@07:23:27.34 delay 2 seconds
@07:23:29.36 ARV.dial :air1
@07:23:29.77 delay 2 seconds
@07:23:31.83 AS.reconfigure ASslow2
@07:23:31.90 AS.seek 0.506ml
@07:23:38.31 delay 2 seconds
@07:23:40.69 ARV.dial :MM1
@07:23:41.33 delay 2 seconds
@07:23:43.35 Adding MM1
@07:23:43.43 AS.seek 0.524ml
@07:24:01.87 delay 25 seconds
@07:24:26.89 ARV.dial :PP2
@07:24:27.31 Adding PP2
@07:24:27.32 delay 2 seconds
@07:24:29.40 AS.seek 0.53ml
@07:24:35.92 delay 10 seconds
@07:24:45.95 ARV.dial :air2
@07:24:46.37 adding ARV air
@07:24:46.38 delay 2 seconds
@07:24:48.46 AS.seek 0.536ml
@07:24:55.20 delay 5 seconds
@07:25:00.22 ARV.dial :carrier
@07:25:01.56 delay 7 seconds
@07:25:08.64 AS.seek 0.594ml
@07:26:07.50 delay 5 seconds
@07:26:12.52 ATV.dial :extSrc
@07:26:13.12 delay 2 seconds
@07:26:15.19 AS.seek 0.6ml
@07:26:21.67 delay 4 seconds
@07:26:25.69 ATV.dial ARV
@07:26:26.28 delay 2 seconds
@07:26:28.30 ARV.dial :carrier
@07:26:28.31 delay 2 seconds
@07:26:30.39 AS.seek 0.65ml
@07:27:21.11 delay 4 seconds
@07:27:25.13 ATV.dial PCR
@07:27:25.95 ASV.dial :waste1
@07:27:25.96 ARV.dial :air1
@07:27:26.40 Positioning Slug by 0.324ml
@07:27:26.48 AS.seek 0.326ml
@07:32:52.84 delay 5 seconds
@07:32:57.86 ATV.dial ARV
@07:32:58.69 ASV.dial :PCRlock
@07:32:59.09 delay 5 seconds
@07:33:04.11 Running ACYPP2 Profile60CIQ2 thermocycling
@07:33:04.12 <PCR> PCR.program Profile60CIQ2
@07:33:04.24 Profile60CIQ2 should complete in < 1:47:50
@07:33:04.25 PCR.run 2:19:47.5
@09:02:26.80 PCR.off
@09:02:27.40 <MFBPCR> Running ACYPP2 MeltProfile60 thermocycling
@09:02:27.42 <PCR> PCR.program MeltProfile60
@09:02:27.52 MeltProfile60 should complete in < 11:55
@09:02:27.53 PCR.run 19:53.75
@09:10:29.26 PCR.off
@09:10:30.51 <MFBPCR> PCR for ACYPP2 completed -- closed /var/log/esp/ACYPP221may0807h.pcr
@09:10:30.58 Cleaning PCR
@09:10:30.59 AFV.select :carrierSolution
@09:10:30.71 delay 2 seconds
@09:10:32.73 AS.reconfigure ASconfig
@09:10:32.79 AS.seek 0.5ml
@09:10:43.11 delay 1 second
@09:10:44.13 AFV.select :holdingCoil
@09:10:44.23 ASV.dial :waste1
@09:10:44.64 ATV.dial :air3
@09:10:46.00 AS.reconfigure ASslow1
@09:10:46.06 AS.seek 0.51ml
@09:10:48.34 delay 2 seconds
@09:10:50.37 ATV.dial :bleach
@09:10:51.03 AS.seek 0.81ml
@09:11:51.62 delay 3 seconds
@09:11:54.64 ATV.dial :air3
@09:11:55.23 delay 3 seconds
@09:11:58.30 AS.seek 0.835ml
@09:12:03.58 delay 3 seconds
@09:12:06.60 ATV.dial :bleach
@09:12:07.25 AS.seek 0.82ml
@09:12:10.66 delay 3 seconds
@09:12:13.69 ARV.dial :waste
@09:12:14.34 delay 3 seconds
@09:12:17.36 ATV.dial :ARV
@09:12:18.28 AS.reconfigure ASconfig
@09:12:18.33 AS.seek 0.47ml
@09:12:38.77 AFV.select :carrierSolution
@09:12:38.89 delay 2 seconds
@09:12:40.91 AS.seek 1ml
@09:13:11.70 delay 1 second
@09:13:12.72 AFV.select :holdingCoil
@09:13:12.82 delay 1 second
@09:13:13.84 AS.reconfigure ASfast
@09:13:13.90 AS.seek 0ml
@09:13:41.22 AFV.select :carrierSolution
@09:13:41.34 delay 2 seconds
@09:13:43.36 AS.reconfigure ASconfig
@09:13:43.41 AS.seek 1ml
@09:14:41.15 delay 1 second
@09:14:42.17 AFV.select :holdingCoil
@09:14:42.27 delay 1 second
@09:14:43.29 AS.reconfigure ASfast
@09:14:43.35 AS.seek 0ml
@09:15:10.69 AFV.select :carrierSolution
@09:15:10.81 delay 2 seconds
@09:15:12.83 AS.reconfigure ASconfig
@09:15:12.89 AS.seek 1ml
@09:16:10.62 delay 1 second
@09:16:11.64 AFV.select :holdingCoil
@09:16:11.74 delay 1 second
@09:16:12.76 AS.reconfigure ASfast
@09:16:12.82 AS.seek 0ml
@09:16:40.16 ARV.dial between carrier and air1
@09:16:40.70 ATV.dial :air3
@09:16:42.05 AS.reconfigure ASslow1
@09:16:42.11 AS.seek 0.01ml
@09:16:44.53 delay 2 seconds
@09:16:46.56 ATV.dial :bleach
@09:16:47.20 AS.seek 0.81ml
@09:19:28.39 delay 3 seconds
@09:19:31.41 ATV.dial :air3
@09:19:32.00 delay 3 seconds
@09:19:35.07 AS.seek 0.835ml
@09:19:40.35 delay 3 seconds
@09:19:43.37 ATV.dial :bleach
@09:19:44.01 AS.seek 0.82ml
@09:19:47.44 delay 3 seconds
@09:19:50.47 ATV.dial PCR
@09:19:50.90 AS.reconfigure ASconfig
@09:19:50.95 AS.seek 0ml
@09:20:38.35 PCR.program :bleachProfile
@09:20:38.42 PCR program should complete in < 2:45
@09:20:38.44 PCR.run 10:00
@09:23:34.61 PCR.off
@09:23:34.65 AFV.select :carrierSolution
@09:23:34.76 delay 2 seconds
@09:23:36.78 AS.seek 1ml
@09:24:34.53 delay 1 second
@09:24:35.55 AFV.select :holdingCoil
@09:24:35.65 delay 1 second
@09:24:36.67 AS.reconfigure ASfast
@09:24:36.73 AS.seek 0ml
@09:25:04.07 delay 3 seconds
@09:25:07.09 AFV.select :carrierSolution
@09:25:07.19 delay 2 seconds
@09:25:09.21 AS.reconfigure ASconfig
@09:25:09.28 AS.seek 1ml
@09:26:07.07 delay 1 second
@09:26:08.09 AFV.select :holdingCoil
@09:26:08.19 delay 1 second
@09:26:09.21 AS.reconfigure ASfast
@09:26:09.27 AS.seek 0ml
@09:26:36.62 delay 3 seconds
@09:26:39.64 AFV.select :carrierSolution
@09:26:39.74 delay 2 seconds
@09:26:41.76 AS.reconfigure ASconfig
@09:26:41.82 AS.seek 1ml
@09:27:39.61 delay 1 second
@09:27:40.63 AFV.select :holdingCoil
@09:27:40.73 delay 1 second
@09:27:41.75 AS.reconfigure ASfast
@09:27:41.81 AS.seek 0ml
@09:28:09.17 delay 3 seconds
@09:28:12.19 AFV.select :carrierSolution
@09:28:12.29 delay 2 seconds
@09:28:14.31 AS.reconfigure ASconfig
@09:28:14.38 AS.seek 1ml
@09:29:12.15 delay 1 second
@09:29:13.17 AFV.select :holdingCoil
@09:29:13.27 delay 1 second
@09:29:14.29 AS.reconfigure ASfast
@09:29:14.35 AS.seek 0ml
@09:29:41.70 delay 3 seconds
@09:29:44.72 AFV.select :carrierSolution
@09:29:44.82 delay 2 seconds
@09:29:46.84 AS.reconfigure ASconfig
@09:29:46.90 AS.seek 1ml
@09:30:44.69 delay 1 second
@09:30:45.72 AFV.select :holdingCoil
@09:30:45.82 delay 1 second
@09:30:46.84 AS.reconfigure ASnorm
@09:30:46.89 AS.seek 0ml
@09:31:37.59 <MAIN> deconEluateRecoveryLine extSrc
@09:31:37.60 ATV.dial :waste
@09:31:38.92 AS.seek 0ml
@09:31:38.99 ATV.dial :air3
@09:31:39.87 AS.reconfigure ASslow1
@09:31:39.92 AS.seek 0.01ml
@09:31:42.27 delay 2 seconds
@09:31:44.30 ATV.dial :bleach
@09:31:44.76 AS.seek 0.31ml
@09:32:45.42 delay 3 seconds
@09:32:48.45 ATV.dial :air3
@09:32:49.06 delay 3 seconds
@09:32:52.13 AS.seek 0.335ml
@09:32:57.51 delay 3 seconds
@09:33:00.53 ATV.dial :bleach
@09:33:01.17 AS.seek 0.32ml
@09:33:04.48 delay 3 seconds
@09:33:07.50 ATV.dial :extSrc
@09:33:08.15 AS.seek 0ml
@09:34:12.88 AFV.select :carrierSolution
@09:34:13.00 delay 2 seconds
@09:34:15.02 AS.reconfigure ASconfig
@09:34:15.08 AS.seek 0.5ml
@09:34:44.06 delay 1 second
@09:34:45.09 AFV.select :holdingCoil
@09:34:45.19 ATV.dial :extSrc
@09:34:45.21 AS.reconfigure ASslow1
@09:34:45.27 AS.seek 0ml
@09:36:26.20 AFV.select :carrierSolution
@09:36:26.32 delay 2 seconds
@09:36:28.34 AS.reconfigure ASconfig
@09:36:28.40 AS.seek 0.5ml
@09:36:57.42 delay 1 second
@09:36:58.44 AFV.select :holdingCoil
@09:36:58.54 ATV.dial :extSrc
@09:36:58.56 AS.reconfigure ASslow1
@09:36:58.62 AS.seek 0ml
@09:38:39.56 AFV.select :carrierSolution
@09:38:39.68 delay 2 seconds
@09:38:41.70 AS.reconfigure ASconfig
@09:38:41.76 AS.seek 0.5ml
@09:39:10.75 delay 1 second
@09:39:11.77 AFV.select :holdingCoil
@09:39:11.87 ATV.dial :extSrc
@09:39:11.89 AS.reconfigure ASslow1
@09:39:11.95 AS.seek 0ml
@09:40:52.89 AFV.select :carrierSolution
@09:40:53.01 delay 2 seconds
@09:40:55.03 AS.reconfigure ASconfig
@09:40:55.09 AS.seek 0.5ml
@09:41:24.10 delay 1 second
@09:41:25.12 AFV.select :holdingCoil
@09:41:25.22 ATV.dial :extSrc
@09:41:25.24 AS.reconfigure ASslow1
@09:41:25.30 AS.seek 0ml
@09:43:06.22 ATV.dial :air2
@09:43:07.62 AS.reconfigure ASslow0
@09:43:07.67 AS.seek 0.5ml
@09:43:58.28 ATV.dial :extSrc
@09:43:59.84 AS.seek 0.02ml
@09:44:48.45 delay 1 second
@09:44:49.47 ATV.dial :waste
@09:44:50.84 AS.reconfigure ASconfig
@09:44:50.89 AS.seek 0ml
@09:44:52.34 deconEluateRecoveryLine extSrc
@09:44:52.35 ATV.dial :waste
@09:44:52.38 AS.reconfigure ASnorm
@09:44:52.44 AS.seek 0ml
@09:44:52.88 ATV.dial :air3
@09:44:53.77 AS.reconfigure ASslow1
@09:44:53.83 AS.seek 0.01ml
@09:44:56.17 delay 2 seconds
@09:44:58.19 ATV.dial :bleach
@09:44:58.65 AS.seek 0.31ml
@09:45:59.35 delay 3 seconds
@09:46:02.37 ATV.dial :air3
@09:46:02.98 delay 3 seconds
@09:46:06.05 AS.seek 0.335ml
@09:46:11.47 delay 3 seconds
@09:46:14.49 ATV.dial :bleach
@09:46:15.12 AS.seek 0.32ml
@09:46:18.42 delay 3 seconds
@09:46:21.44 ATV.dial :extSrc
@09:46:22.09 AS.seek 0ml
@09:47:26.72 AFV.select :carrierSolution
@09:47:26.89 delay 2 seconds
@09:47:28.94 AS.reconfigure ASconfig
@09:47:29.00 AS.seek 0.5ml
@09:47:58.04 delay 1 second
@09:47:59.06 AFV.select :holdingCoil
@09:47:59.16 ATV.dial :extSrc
@09:47:59.18 AS.reconfigure ASslow1
@09:47:59.24 AS.seek 0ml
@09:49:40.14 AFV.select :carrierSolution
@09:49:40.26 delay 2 seconds
@09:49:42.28 AS.reconfigure ASconfig
@09:49:42.34 AS.seek 0.5ml
@09:50:11.37 delay 1 second
@09:50:12.39 AFV.select :holdingCoil
@09:50:12.49 ATV.dial :extSrc
@09:50:12.51 AS.reconfigure ASslow1
@09:50:12.57 AS.seek 0ml
@09:51:53.41 AFV.select :carrierSolution
@09:51:53.53 delay 2 seconds
@09:51:55.55 AS.reconfigure ASconfig
@09:51:55.61 AS.seek 0.5ml
@09:52:24.65 delay 1 second
@09:52:25.67 AFV.select :holdingCoil
@09:52:25.77 ATV.dial :extSrc
@09:52:25.79 AS.reconfigure ASslow1
@09:52:25.85 AS.seek 0ml
@09:54:06.81 AFV.select :carrierSolution
@09:54:06.93 delay 2 seconds
@09:54:08.95 AS.reconfigure ASconfig
@09:54:09.01 AS.seek 0.5ml
@09:54:38.07 delay 1 second
@09:54:39.09 AFV.select :holdingCoil
@09:54:39.19 ATV.dial :extSrc
@09:54:39.21 AS.reconfigure ASslow1
@09:54:39.27 AS.seek 0ml
@09:56:20.22 ATV.dial :air2
@09:56:21.62 AS.reconfigure ASslow0
@09:56:21.67 AS.seek 0.5ml
@09:57:12.28 ATV.dial :extSrc
@09:57:13.84 AS.seek 0.02ml
@09:58:02.45 delay 1 second
@09:58:03.47 ATV.dial :waste
@09:58:04.82 AS.reconfigure ASconfig
@09:58:04.87 AS.seek 0ml
@09:58:06.32 deconEluateRecoveryLine extSrc
@09:58:06.33 ATV.dial :waste
@09:58:06.36 AS.reconfigure ASnorm
@09:58:06.42 AS.seek 0ml
@09:58:06.55 ATV.dial :air3
@09:58:07.44 AS.reconfigure ASslow1
@09:58:07.51 AS.seek 0.01ml
@09:58:09.81 delay 2 seconds
@09:58:11.84 ATV.dial :bleach
@09:58:12.31 AS.seek 0.31ml
@09:59:13.03 delay 3 seconds
@09:59:16.05 ATV.dial :air3
@09:59:16.64 delay 3 seconds
@09:59:19.71 AS.seek 0.335ml
@09:59:25.00 delay 3 seconds
@09:59:28.03 ATV.dial :bleach
@09:59:28.67 AS.seek 0.32ml
@09:59:32.05 delay 3 seconds
@09:59:35.08 ATV.dial :extSrc
@09:59:35.73 AS.seek 0ml
@10:00:40.49 AFV.select :carrierSolution
@10:00:40.61 delay 2 seconds
@10:00:42.63 AS.reconfigure ASconfig
@10:00:42.69 AS.seek 0.5ml
@10:01:11.68 delay 1 second
@10:01:12.70 AFV.select :holdingCoil
@10:01:12.80 ATV.dial :extSrc
@10:01:12.82 AS.reconfigure ASslow1
@10:01:12.88 AS.seek 0ml
@10:02:53.81 AFV.select :carrierSolution
@10:02:53.93 delay 2 seconds
@10:02:55.95 AS.reconfigure ASconfig
@10:02:56.01 AS.seek 0.5ml
@10:03:25.09 delay 1 second
@10:03:26.11 AFV.select :holdingCoil
@10:03:26.21 ATV.dial :extSrc
@10:03:26.23 AS.reconfigure ASslow1
@10:03:26.29 AS.seek 0ml
@10:05:07.20 AFV.select :carrierSolution
@10:05:07.32 delay 2 seconds
@10:05:09.34 AS.reconfigure ASconfig
@10:05:09.40 AS.seek 0.5ml
@10:05:38.41 delay 1 second
@10:05:39.43 AFV.select :holdingCoil
@10:05:39.53 ATV.dial :extSrc
@10:05:39.55 AS.reconfigure ASslow1
@10:05:39.61 AS.seek 0ml
@10:07:20.42 AFV.select :carrierSolution
@10:07:20.54 delay 2 seconds
@10:07:22.56 AS.reconfigure ASconfig
@10:07:22.62 AS.seek 0.5ml
@10:07:51.66 delay 1 second
@10:07:52.68 AFV.select :holdingCoil
@10:07:52.78 ATV.dial :extSrc
@10:07:52.80 AS.reconfigure ASslow1
@10:07:52.86 AS.seek 0ml
@10:09:33.70 ATV.dial :air2
@10:09:35.09 AS.reconfigure ASslow0
@10:09:35.15 AS.seek 0.5ml
@10:10:25.78 ATV.dial :extSrc
@10:10:27.34 AS.seek 0.02ml
@10:11:15.92 delay 1 second
@10:11:16.94 ATV.dial :waste
@10:11:18.31 AS.reconfigure ASconfig
@10:11:18.36 AS.seek 0ml
@10:11:19.86 <mwj> Session Ended