@11:17:52.64PST22-Jan-14 ESP2 v3.20 I2C Gateway v3.01 @11:17:55.88
Configuration for neo by maro@mbari.org /home/esp/esp2/shallow/neo/configure.rb @11:17:55.97 Configuration for MFB10 by preston@mbari.org /home/esp/esp2/mfb/MFB10/configure.rb @11:17:59.21 Sleepy.power :raw,:ON @11:17:59.92 SPE.coast @11:17:59.96 SPE.configure SPEconfig @11:17:59.99 AS.stop @11:18:00.02 AS.configure ASconfig @11:18:00.05 CC.stop @11:18:00.07 CC.configure CCconfig @11:18:00.10 CS.stop @11:18:00.12 CS.configure CSconfig @11:18:00.15 PC.stop @11:18:00.17 PC.configure PCconfig @11:18:00.20 PS.stop @11:18:00.22 PS.configure PSconfig @11:18:00.28 Forearm.stop @11:18:00.30 Forearm.configure LooseForearm @11:18:00.33 Elbow.stop @11:18:00.35 Elbow.configure LooseElbow @11:18:00.41 SC.stop @11:18:00.43 SC.configure SCloose @11:18:00.46 SE.stop @11:18:00.48 SE.configure SEconfig @11:18:00.51 SS.stop @11:18:00.53 SS.configure SSconfig @11:18:00.56 CH.coast @11:18:00.60 CH.configure CHconfig @11:18:00.63 PH.coast @11:18:00.67 PH.configure PHconfig @11:18:00.71 PCR.off @11:18:00.73 PCR.pidtemp= 50,0.1,0,300,25,25 @11:18:00.76 PCR.caltemp= 6.6661,0.0344,0.0 @11:18:00.79 All dwarves are running firmware version 3.74 @11:18:01.43 CTD.latitude = 36.8044 Degrees North in seawater @11:18:01.44 Initializing Sensors @11:18:01.47 GoodNews.email "Starting w/pucks in tube 2\n Ending 13:00PST22-Feb-14",Subject => "14jan21SCCWRP begun" @11:18:02.59
Asleep til 11:25 AM 1/22/14 @11:18:02.64 Sleeping until 11:25 @11:18:02.66 Sleepy.power :raw,:OFF @11:18:02.67 Sleepy.power :dwarf,:OFF @11:18:02.68 Shutdown @11:18:14.06 GoodNews.email "Then, in tube 2: wqPCR\n/home/esp/esp2/mission/14jan21SCCWRP.rb:49",Subject => "Asleep until 11:25" @11:18:16.51 Net::SMTPServerBusy in Qmail -- 421 4.4.5 Server busy, try again later. (mx.google.com) z10sm51357115pas.6 - gsmtp @11:18:24.05 Session Begun @11:18:26.48 Instrument::ReadTimeout in pollContext -- Could not get prompt from CTD on ctd @11:18:26.50 BadNews.email "Could not get prompt from CTD on ctd",Subject => "Instrument::ReadTimeout in pollContext" @11:18:45.53 Session Begun @11:18:48.52 -> showlog @11:22:41.10 -> showlog @11:25:00.33
Powering up Dwarves @11:25:05.31 Sleepy.power :raw,:ON @11:25:05.79 SPE.configure SPEconfig @11:25:05.83 AS.configure ASconfig @11:25:05.88 CC.configure CCconfig @11:25:05.93 CS.configure CSconfig @11:25:05.98 PC.configure PCconfig @11:25:06.03 PS.configure PSconfig @11:25:06.08 Forearm.configure LooseForearm @11:25:06.13 Elbow.configure LooseElbow @11:25:06.18 SC.configure SCloose @11:25:06.23 SE.configure SEconfig @11:25:06.28 SS.configure SSconfig @11:25:06.33 CH.configure CHconfig @11:25:06.36 PH.configure PHconfig @11:25:06.41 Valve.reset @11:25:06.82 PCR.off @11:25:06.86 PCR.pidtemp= 50,0.1,0,300,25,25 @11:25:06.90 PCR.caltemp= 6.6661,0.0344,0.0 @11:25:07.03 Intake.select :close @11:25:07.04 Exhaust.select :close @11:25:07.05 CTV.dial :air @11:25:07.13 PSV.dial :air @11:25:07.17
PC.reconfigure PCconfig @11:25:07.25 PC.home.seek open @11:25:07.27 CSV.dial :waste1 @11:25:07.39 PS.reconfigure PSconfig @11:25:07.44 PS.home.seek 0.000ml @11:25:07.82 SSV.dial :spit,avoiding => exhaust @11:25:07.95 CS.reconfigure CSfast @11:25:07.98 SS.reconfigure SSconfig @11:25:08.09 CS.home.seek 0.000ml @11:25:08.12 SS.home.seek 0.00ml @11:25:08.22 CC.reconfigure CCconfig @11:25:08.27 CC.home.seek open @11:25:17.75 PS.coast @11:25:17.85
CC.seek open @11:25:17.90 PC.seek open @11:25:18.06
Forearm.reconfigure LooseForearm @11:25:18.11 Forearm.home.seek retract @11:25:18.84 Hand.grip @11:25:18.87
Elbow.jog -1500 @11:25:19.39 Elbow.reconfigure LooseElbow @11:25:19.44 Elbow.home.seek clear @11:25:24.15 SE.reconfigure SEconfig @11:25:24.20 SE.home.seek down @11:25:24.80 SC.reconfigure SCsloppy @11:25:24.85 SC.home.seek initial @11:25:28.58 Forearm.seek retract @11:25:28.67 Elbow.seek hammer @11:25:29.89 Forearm.reconfigure TightForearm @11:25:29.96 Forearm.seek 2 @11:25:31.13 Forearm.seek retract @11:25:32.27 Forearm.reconfigure LooseForearm @11:25:32.72PST22-Jan-14 ===> STARTED wqPCR (phase #1) <=== @11:25:32.82 Starting mfb @11:25:32.83 pcrBAC sampling at most 250ml, wcr at most 1000ml @11:25:32.86 BACfans.email "Can@11:25:32, 21.8C, 46% humidity, 14.4psia, 13.729V, 0.665A, 0.665A avg, 9.13W\nCTD data unavailable\nISUS data unavailable",Subject => "pcrBAC sampling at most 250ml, wcr at most 1000ml" @11:25:34.19
Loading sh1 puck from tube 2 into Collection Clamp @11:25:34.29 Readying the MFB @11:25:34.32 Homing MFB Syringe AFV.select :holdingCoil @11:25:34.40
Hand.release @11:25:34.49 ATV.dial :air2 @11:25:35.38
SC.reconfigure SCloose @11:25:35.50 AS.reconfigure ASconfig @11:25:35.55 AS.home.seek home @11:25:35.66
Elbow.reconfigure TightElbow @11:25:35.72 Elbow.seek 2 @11:25:35.98 Forearm.reconfigure TightForearm @11:25:36.04 Forearm.seek 2 + 800 counts @11:25:37.09 SE.reconfigure SEpick @11:25:37.16 SE.seek beamBreak @11:25:39.27 SE.configure SEconfig @11:25:39.42 Elbow.seek 2 @11:25:39.66 Forearm.seek 2 + 300 counts @11:25:39.88 Forearm.reconfigure LooseForearm @11:25:39.93 Hand.grip @11:25:40.73 SE.seek down @11:25:40.89
Forearm.seek retract @11:25:41.90 Elbow.seek CC @11:25:42.74 SE.coast @11:25:43.22 AFV.select :carrierSolution @11:25:43.32 delay 2 seconds @11:25:43.49
Elbow.reconfigure LooseElbow @11:25:43.59 Forearm.reconfigure TightForearm @11:25:43.64 Forearm.seek CC @11:25:44.79 CC.seek closed @11:25:45.34 AS.seek 1.000ml @11:25:48.65
Collection Clamp onSpring/offSpring for 4/21 samples, on/off torque 1.99 @11:25:48.66 Hand.release @11:25:49.50 Forearm.reconfigure LooseForearm @11:25:49.55 Forearm.seek retract @11:25:50.52 SC.coast @11:25:50.57 collectSample 250ml w/bubble point of 55psi @11:25:50.58 Priming 7ml @11:25:50.60 Intake.select :close @11:25:50.62 Exhaust.select :close @11:25:50.65 SS.hold @11:25:50.83 Calibrating pressure and temperature sensors @11:25:50.84 CTV.dial :air @11:25:50.86 SSV.dial :spit,avoiding => exhaust @11:25:50.90 SS.reconfigure SSfast @11:25:50.96 SS.seek 5.00ml @11:25:53.80 SSV.dial CBV @11:25:53.83
CBV.dial SSV @11:25:55.17 delay 3.8 seconds @11:25:59.06 SSV.dial :spit @11:26:00.37 SS.seek 0.00ml @11:26:02.91 SSV.dial CBV @11:26:04.22 CTV.dial :intake @11:26:04.98 SSV.dial CBV @11:26:04.99
CBV.dial SSV @11:26:05.00 Allowing 55s for 7.0ml of remaining 7.0ml @11:26:05.04 Intake.select :open @11:26:07.22 Intake @41.9psia, vacuum from 20.0 to 24.0psi @11:26:07.23 SS.reconfigure forSampling @11:26:07.31 SS.seek 7.00ml @11:26:10.34 Vacuum too high -- waiting up to 40s @11:26:10.60 Intake @36.2psia, vacuum from 20.0 to 24.0psi @11:26:10.61 SS.reconfigure forSampling @11:26:10.69 SS.seek 7.00ml @11:26:14.09 Stroke completed -- waiting for pressure to equalize ... @11:26:14.72 Intake.select :close @11:26:14.87 SSV.dial :exhaust @11:26:15.64 Exhaust.select :open @11:26:15.75 SS.reconfigure SSexhale @11:26:15.80 SS.seek 0.00ml @11:26:19.46 Exhaust.select :close @11:26:19.57 Sampling 250.0ml @11:26:19.58 Intake.select :close @11:26:19.59 Exhaust.select :close @11:26:19.61 SS.reconfigure SSconfig @11:26:19.68 SS.hold @11:26:19.85 CTV.dial :intake @11:26:19.86 SSV.dial CBV @11:26:19.90
CBV.dial SSV @11:26:20.70 Allowing 185s for 25.0ml of remaining 250.0ml @11:26:20.71 Intake.select :open @11:26:22.87 Intake @42.0psia, vacuum from 20.0 to 24.0psi @11:26:22.88 SS.reconfigure forSampling @11:26:22.96 SS.seek 25.00ml @11:26:37.11 delay 1 second @11:26:38.12 AFV.select :holdingCoil @11:26:38.22
Stroke completed -- waiting for pressure to equalize ... @11:26:38.25 delay 1 second @11:26:38.86
Intake.select :close @11:26:39.01 SSV.dial :exhaust @11:26:39.26 ATV.dial :waste @11:26:39.78
Exhaust.select :open @11:26:40.42 SS.reconfigure SSexhale @11:26:40.44 AS.reconfigure ASnorm @11:26:40.53
SS.seek 0.00ml @11:26:40.55 AS.home.seek empty @11:26:49.34
Exhaust.select :close @11:26:49.45 SSV.dial CBV @11:26:49.47
CBV.dial SSV @11:26:50.27 Allowing 185s for 25.0ml of remaining 225.0ml @11:26:50.29 Intake.select :open @11:26:52.45 Intake @41.6psia, vacuum from 20.0 to 24.0psi @11:26:52.46 SS.reconfigure forSampling @11:26:52.54 SS.seek 25.00ml @11:27:07.78 Stroke completed -- waiting for pressure to equalize ... @11:27:08.42 Intake.select :close @11:27:08.57 SSV.dial :exhaust @11:27:09.34 Exhaust.select :open @11:27:09.45 SS.reconfigure SSexhale @11:27:09.50 SS.seek 0.00ml @11:27:18.20 Exhaust.select :close @11:27:18.31 SSV.dial CBV @11:27:18.33
CBV.dial SSV @11:27:19.12 Allowing 185s for 25.0ml of remaining 200.0ml @11:27:19.13 Intake.select :open @11:27:21.29 Intake @41.1psia, vacuum from 20.0 to 24.0psi @11:27:21.30 SS.reconfigure forSampling @11:27:21.38 SS.seek 25.00ml @11:27:33.89 ATV.dial :waste @11:27:33.90 ARV.dial between carrier and air1 @11:27:34.00 ASV.dial :waste1 @11:27:36.64
Stroke completed -- waiting for pressure to equalize ... @11:27:37.28 Intake.select :close @11:27:37.43 SSV.dial :exhaust @11:27:38.20 Exhaust.select :open @11:27:38.31 SS.reconfigure SSexhale @11:27:38.36 SS.seek 0.00ml @11:27:47.22 Exhaust.select :close @11:27:47.33 SSV.dial CBV @11:27:47.35
CBV.dial SSV @11:27:48.15 Allowing 185s for 25.0ml of remaining 175.0ml @11:27:48.16 Intake.select :open @11:27:50.32 Intake @40.7psia, vacuum from 20.0 to 24.0psi @11:27:50.33 SS.reconfigure forSampling @11:27:50.41 SS.seek 25.00ml @11:28:05.68 Stroke completed -- waiting for pressure to equalize ... @11:28:06.32 Intake.select :close @11:28:06.47 SSV.dial :exhaust @11:28:07.25 Exhaust.select :open @11:28:07.36 SS.reconfigure SSexhale @11:28:07.41 SS.seek 0.00ml @11:28:16.26 Exhaust.select :close @11:28:16.37 SSV.dial CBV @11:28:16.39
CBV.dial SSV @11:28:17.19 Allowing 185s for 25.0ml of remaining 150.0ml @11:28:17.20 Intake.select :open @11:28:19.42 Intake @40.5psia, vacuum from 20.0 to 24.0psi @11:28:19.43 SS.reconfigure forSampling @11:28:19.49 SS.seek 25.00ml @11:28:34.85 Stroke completed -- waiting for pressure to equalize ... @11:28:35.49 Intake.select :close @11:28:35.64 SSV.dial :exhaust @11:28:36.42 Exhaust.select :open @11:28:36.53 SS.reconfigure SSexhale @11:28:36.58 SS.seek 0.00ml @11:28:45.35 Exhaust.select :close @11:28:45.46 SSV.dial CBV @11:28:45.48
CBV.dial SSV @11:28:46.28 Allowing 185s for 25.0ml of remaining 125.0ml @11:28:46.30 Intake.select :open @11:28:48.46 Intake @40.1psia, vacuum from 20.0 to 24.0psi @11:28:48.47 SS.reconfigure forSampling @11:28:48.54 SS.seek 25.00ml @11:29:03.91 Stroke completed -- waiting for pressure to equalize ... @11:29:04.55 Intake.select :close @11:29:04.69 SSV.dial :exhaust @11:29:05.46 Exhaust.select :open @11:29:05.57 SS.reconfigure SSexhale @11:29:05.62 SS.seek 0.00ml @11:29:14.62 Exhaust.select :close @11:29:14.73 SSV.dial CBV @11:29:14.75
CBV.dial SSV @11:29:15.54 Allowing 185s for 25.0ml of remaining 100.0ml @11:29:15.56 Intake.select :open @11:29:17.72 Intake @39.6psia, vacuum from 20.0 to 24.0psi @11:29:17.73 SS.reconfigure forSampling @11:29:17.81 SS.seek 25.00ml @11:29:33.12 Stroke completed -- waiting for pressure to equalize ... @11:29:33.76 Intake.select :close @11:29:33.91 SSV.dial :exhaust @11:29:34.69 Exhaust.select :open @11:29:34.80 SS.reconfigure SSexhale @11:29:34.86 SS.seek 0.00ml @11:29:43.69 Exhaust.select :close @11:29:43.80 SSV.dial CBV @11:29:43.82
CBV.dial SSV @11:29:44.60 Allowing 185s for 25.0ml of remaining 75.0ml @11:29:44.62 Intake.select :open @11:29:46.78 Intake @39.3psia, vacuum from 20.0 to 24.0psi @11:29:46.79 SS.reconfigure forSampling @11:29:46.87 SS.seek 25.00ml @11:30:02.30 Stroke completed -- waiting for pressure to equalize ... @11:30:02.94 Intake.select :close @11:30:03.09 SSV.dial :exhaust @11:30:03.86 Exhaust.select :open @11:30:03.97 SS.reconfigure SSexhale @11:30:04.02 SS.seek 0.00ml @11:30:13.18 Exhaust.select :close @11:30:13.29 SSV.dial CBV @11:30:13.31
CBV.dial SSV @11:30:14.09 Allowing 185s for 25.0ml of remaining 50.0ml @11:30:14.10 Intake.select :open @11:30:16.26 Intake @38.9psia, vacuum from 20.0 to 24.0psi @11:30:16.27 SS.reconfigure forSampling @11:30:16.35 SS.seek 25.00ml @11:30:31.63 Stroke completed -- waiting for pressure to equalize ... @11:30:32.27 Intake.select :close @11:30:32.42 SSV.dial :exhaust @11:30:33.19 Exhaust.select :open @11:30:33.30 SS.reconfigure SSexhale @11:30:33.36 SS.seek 0.00ml @11:30:42.16 Exhaust.select :close @11:30:42.27 SSV.dial CBV @11:30:42.29
CBV.dial SSV @11:30:43.08 Allowing 185s for 25.0ml of remaining 25.0ml @11:30:43.10 Intake.select :open @11:30:45.26 Intake @38.5psia, vacuum from 20.0 to 24.0psi @11:30:45.27 SS.reconfigure forSampling @11:30:45.35 SS.seek 24.99ml @11:31:00.85 Stroke completed -- waiting for pressure to equalize ... @11:31:01.49 Intake.select :close @11:31:01.65 SSV.dial :exhaust @11:31:02.42 Exhaust.select :open @11:31:02.54 SS.reconfigure SSexhale @11:31:02.59 SS.seek 0.00ml @11:31:11.95 Exhaust.select :close @11:31:12.06 Sampled 250.0ml @11:31:12.07 collectSample: Complete @11:31:12.08 Evacuating Puck (bubblePt=55.0psi, delay=15.0s) @11:31:12.10 Intake.select :close @11:31:12.18 CSV.dial :waste @11:31:12.23 CS.seek 0.000ml @11:31:12.32
CBV.dial SSV @11:31:12.34 SSV.dial CBV,avoiding => exhaust @11:31:12.41 CSV.dial :air @11:31:13.14 CS.seek 10.000ml @11:31:14.20
CTV.dial :air @11:31:14.97 delay 15 seconds @11:31:29.97 SSV.dial :spit,avoiding => exhaust @11:31:31.29 CTV.dial CSV,avoiding => intake @11:31:31.31
SS.seek 0.00ml @11:31:31.42 SSV.dial CBV,avoiding => exhaust @11:31:36.48 CBV.dial between CSV and waste2b @11:31:37.44 CTV.dial CSV,avoiding => air @11:31:37.46
CSV.dial CTV @11:31:38.55 CS.reconfigure CSpressurize @11:31:38.61 CS.seek 0.000ml @11:32:03.19 BubblePoint: Intake @70.1psia, Outlet @15.2psia @11:32:03.20 SS.reconfigure SSconfig @11:32:03.27 SS.seek 10.00ml @11:32:09.08 delay 15 seconds @11:32:24.18 Evacuated: Intake @68.9psia, Outlet @-0.4psia @11:32:24.19 SSV.dial :spit,avoiding => exhaust @11:32:25.52 SS.reconfigure SSexhale @11:32:25.57 SS.seek 0.00ml @11:32:31.11 SS.reconfigure SSfast @11:32:31.17 SS.seek 5.00ml @11:32:34.34 SSV.dial CBV,avoiding => exhaust @11:32:34.36
CBV.dial SSV @11:32:35.71 CTV.dial :air,avoiding => intake @11:32:37.04 CSV.dial CTV @11:32:37.06
SS.reconfigure SSconfig @11:32:37.13 SS.seek 10.00ml @11:32:38.05 CSV.dial :air @11:32:38.59 CS.reconfigure CSfast @11:32:38.64 CS.seek 0.000ml @11:32:40.32
SSV.dial :spit,avoiding => exhaust @11:32:40.67 CS.coast @11:32:41.66
SS.reconfigure SSexhale @11:32:41.71 SS.seek 0.00ml @11:32:46.78 SS.reconfigure SSfast @11:32:46.84 SS.seek 5.00ml @11:32:49.94 SSV.dial CBV,avoiding => exhaust @11:32:49.96
CBV.dial SSV @11:32:52.28 SSV.dial :spit,avoiding => exhaust @11:32:53.38 Session Ended @11:32:53.60
SS.reconfigure SSconfig @11:32:53.64 SS.seek 0.00ml @11:32:56.98 SS.coast @11:32:57.02 Evacuating Puck (bubblePt=55.0psi, delay=15.0s) @11:32:57.03 Intake.select :close @11:32:57.11 CSV.dial :waste @11:32:57.21
SSV.dial :exhaust @11:32:57.85 CS.seek 0.000ml @11:32:57.96
Exhaust.select :open @11:32:58.06 SS.reconfigure SSexhale @11:32:58.11 SS.seek 0.00ml @11:32:58.30 CSV.dial :air @11:32:58.81
Exhaust.select :close @11:32:58.93 SSV.dial CBV,avoiding => exhaust @11:32:58.95
CBV.dial SSV @11:32:59.32 CS.seek 10.000ml @11:33:00.82
CTV.dial :air @11:33:00.83 delay 15 seconds @11:33:15.84 SSV.dial :spit,avoiding => exhaust @11:33:17.16 CTV.dial CSV,avoiding => intake @11:33:17.18
SS.seek 0.00ml @11:33:17.29 SSV.dial CBV,avoiding => exhaust @11:33:22.75 CBV.dial between CSV and waste2b @11:33:23.70 CSV.dial between air and CTV @11:33:24.10 CS.seek 8.137ml @11:33:29.15 CTV.dial CSV,avoiding => air @11:33:29.16
CSV.dial CTV @11:33:29.48 CS.reconfigure CSpressurize @11:33:29.53 CS.seek 0.000ml @11:33:50.73 CSV.dial :air @11:33:51.26 CS.reconfigure CSfast @11:33:51.30 CS.seek 10.000ml @11:34:14.49 CSV.dial between air and CTV @11:34:15.10 CS.seek 2.953ml @11:34:31.71 CTV.dial CSV,avoiding => air @11:34:31.72
CSV.dial CTV @11:34:32.13 CS.reconfigure CSpressurize @11:34:32.17 CS.seek 0.000ml @11:34:38.31 BubblePoint: Intake @68.8psia, Outlet @14.1psia @11:34:38.32 SS.reconfigure SSconfig @11:34:38.37 SS.seek 10.00ml @11:34:44.41 delay 15 seconds @11:34:59.51 Evacuated: Intake @68.0psia, Outlet @-0.2psia @11:34:59.52 SSV.dial :spit,avoiding => exhaust @11:35:00.86 SS.reconfigure SSexhale @11:35:00.90 SS.seek 0.00ml @11:35:05.94 SS.reconfigure SSfast @11:35:05.99 SS.seek 5.00ml @11:35:09.09 SSV.dial CBV,avoiding => exhaust @11:35:09.11
CBV.dial SSV @11:35:10.45 CTV.dial :air,avoiding => intake @11:35:11.77 CSV.dial CTV @11:35:11.79
SS.reconfigure SSconfig @11:35:11.85 SS.seek 10.00ml @11:35:12.79 CSV.dial :air @11:35:13.33 CS.reconfigure CSfast @11:35:13.37 CS.seek 0.000ml @11:35:15.09
SSV.dial :spit,avoiding => exhaust @11:35:15.86 CS.coast @11:35:16.41
SS.reconfigure SSexhale @11:35:16.46 SS.seek 0.00ml @11:35:21.73 SS.reconfigure SSfast @11:35:21.78 SS.seek 5.00ml @11:35:24.84 SSV.dial CBV,avoiding => exhaust @11:35:24.86
CBV.dial SSV @11:35:27.17 SSV.dial :spit,avoiding => exhaust @11:35:28.48 SS.reconfigure SSconfig @11:35:28.52 SS.seek 0.00ml @11:35:31.84 SS.coast @11:35:31.88 Lysing: Adding 1.5ml lysis @11:35:31.89 CBV.dial between CSV and waste1,avoiding => DBV @11:35:33.39 CTV.dial CSV @11:35:34.67 CSV.dial CSR @11:35:35.47 CSR.select :lysis @11:35:35.57 CS.reconfigure CSconfig @11:35:35.62 CS.seek 1.000ml @11:35:38.92 delay 5 seconds @11:35:43.93 CSR.select :air @11:35:44.09 CS.seek 6.000ml @11:35:57.55 delay 2 seconds @11:35:59.56 CSV.dial :waste1 @11:36:00.84 CS.reconfigure CSfast @11:36:00.88 CS.seek empty @11:36:14.98 CSV.dial CSR @11:36:16.24 CSR.select :lysis @11:36:16.34 CS.reconfigure CSconfig @11:36:16.39 CS.seek 1.500ml @11:36:21.11 delay 5 seconds @11:36:26.12 CSR.select :air @11:36:26.28 CS.seek 4.500ml @11:36:34.70 delay 2 seconds @11:36:36.71 CSV.dial CTV @11:36:37.23 CS.seek 1.275ml @11:36:46.24 Heating sh1 puck from tube 2 in Collection Clamp to 85.0C for 5:00 @11:36:46.26 CH.seek 85.0C,8:00 @11:38:36.17 CH.hold 5:00,85.0C @11:43:36.29 Collection Puck Heater at 84.9C after lysing @11:43:36.30 CS.seek 0.000ml @11:43:40.41 CTV.dial between spare and air,avoiding => intake @11:43:41.42 CSV.dial :air @11:43:41.86 CS.seek 5.000ml @11:43:55.46 CSV.dial CTV @11:43:55.96 delay 2 seconds @11:43:57.98 CSV.dial :air @11:43:58.49 delay 2 seconds @11:44:00.51 CSV.dial CTV @11:44:01.03 delay 2 seconds @11:44:03.04 CSV.dial :air @11:44:03.56 delay 2 seconds @11:44:05.57 CS.seek 0.000ml @11:44:19.09 Recovering Lysate into Collection syringe @11:44:19.10 CSV.dial CBV @11:44:20.10 CBV.dial CSV @11:44:20.46 CS.seek 5.000ml @11:44:33.94 delay 5 seconds @11:44:39.09 CTV.dial :air @11:44:39.59 CS.seek 6.999ml @11:44:45.43 delay 5 seconds @11:44:50.44 CSV.dial :air2 @11:44:50.88 CS.seek 10.000ml @11:44:59.27 CSV.dial between air and CTV,avoiding => CBV @11:45:00.41 Lysing Complete @11:45:00.48 Storing sh1 puck in Collection Clamp to tube 1 @11:45:00.62 SC.reconfigure SCtight @11:45:00.67 SC.seek 1 @11:45:02.96 SC.reconfigure SCloose @11:45:03.14 Elbow.reconfigure TightElbow @11:45:03.19 Elbow.seek 1 @11:45:04.81 Forearm.reconfigure TightForearm @11:45:04.86 Forearm.seek 1 + 800 counts @11:45:05.94 SE.reconfigure SEpick @11:45:06.01 SE.seek beamBreak @11:45:08.83 SE.configure SEconfig @11:45:08.88 SE.seek place @11:45:09.35 Forearm.reconfigure LooseForearm @11:45:09.40 Forearm.seek retract @11:45:10.32 Elbow.seek CC @11:45:12.55 Elbow.reconfigure LooseElbow @11:45:12.65 Forearm.seek CC @11:45:13.62 CC.seek sealed @11:45:14.55 Hand.grip @11:45:15.29 CC.seek open @11:45:18.66 Forearm.seek retract @11:45:19.62 Elbow.reconfigure TightElbow @11:45:19.66 Elbow.seek 1 @11:45:21.26 Elbow.reconfigure LimpElbow @11:45:21.32 Forearm.seek 1 @11:45:22.37 Elbow.hold @11:45:22.43 Elbow.reconfigure TightElbow @11:45:22.48 Hand.release @11:45:23.25 Forearm.coast @11:45:23.30 Forearm.seek 1 + 326 counts @11:45:23.54 Forearm.reconfigure TightForearm @11:45:23.59 Forearm.seek 1 + 800 counts @11:45:23.91 SE.seek down @11:45:25.01
Forearm.reconfigure LooseForearm @11:45:25.06 Forearm.seek retract @11:45:26.60 SE.coast @11:45:26.64
SC.coast @11:45:26.67 Tranferring lysate to PRV @11:45:26.68 CSV.dial :air @11:45:26.98 CS.seek 10.000ml @11:45:27.05 CSV.dial between air and CTV @11:45:27.44 AIV.dial :bypass @11:45:27.52 Beginning samplePassC @11:45:27.53 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @11:45:27.70 Elbow.seek FlushPuck::Garage @11:45:29.41 Elbow.reconfigure LooseElbow @11:45:29.50 Forearm.seek garage @11:45:30.55 Hand.grip @11:45:31.38 Forearm.seek retract @11:45:32.42 Elbow.reconfigure TightElbow @11:45:32.47 Elbow.seek CC @11:45:35.30 Elbow.reconfigure LooseElbow @11:45:35.39 Forearm.reconfigure TightForearm @11:45:35.44 Forearm.seek CC @11:45:36.52 CC.seek closed @11:45:40.29 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.93 @11:45:40.36 Hand.release @11:45:41.21 Forearm.reconfigure LooseForearm @11:45:41.26 Forearm.seek retract @11:45:42.25 AWV.select :air @11:45:42.26 AIV.dial :bypass @11:45:42.27 PSV.dial PSR @11:45:43.60 PSR.select :flush @11:45:43.72 PS.seek 1.500ml @11:45:59.84 delay 5 seconds @11:46:04.85 PSV.dial :waste1 @11:46:05.31 PS.seek 0.000ml @11:46:21.30 PSV.dial PSR @11:46:21.77 PSR.select :air @11:46:21.88 PS.seek 2.500ml @11:46:48.22 CBV.dial PRV @11:46:48.23 PRV.dial CBV @11:46:49.02
PSV.dial :mixloop @11:46:50.04 CTV.dial :air,avoiding => intake @11:46:50.05 PTV.dial :PRVmixing @11:46:51.34 PS.seek 0.000ml @11:47:17.55 PSV.dial :air @11:47:18.01 PS.seek 2.500ml @11:47:44.27 PSV.dial :mixloop @11:47:44.76 PS.seek 0.000ml @11:48:10.99 PSV.dial :air @11:48:11.47 PS.seek 2.500ml @11:48:37.77 PSV.dial :mixloop @11:48:38.25 PS.seek 0.000ml @11:49:05.23 Garaging FlushPuck in Collection Clamp @11:49:05.31 Elbow.reconfigure TightElbow @11:49:05.36 Elbow.seek CC @11:49:05.47 Elbow.reconfigure LooseElbow @11:49:05.57 Forearm.seek CC @11:49:06.55 CC.seek sealed @11:49:07.51 Hand.grip @11:49:08.28 CC.seek open @11:49:11.60 Forearm.seek retract @11:49:12.55 Elbow.reconfigure TightElbow @11:49:12.60 Elbow.seek garage @11:49:15.37 Elbow.reconfigure LooseElbow @11:49:15.46 Forearm.reconfigure TightForearm @11:49:15.51 Forearm.seek garage @11:49:16.68 Hand.release @11:49:17.45 Forearm.reconfigure LooseForearm @11:49:17.50 Forearm.seek retract @11:49:18.55 Connecting PS to CS to transfer sample @11:49:18.56 CBV.dial AIV @11:49:18.57 AIV.dial :bypass @11:49:18.60 PSV.dial :mixloop @11:49:18.62 PTV.dial :PRVmixing @11:49:18.63 PRV.dial AIV @11:49:18.65 CTV.dial :air,avoiding => intake @11:49:18.68 Loading lyfil puck from tube 2 into Collection Clamp @11:49:18.88 SC.reconfigure SCtight @11:49:18.93 SC.seek 2 @11:49:21.17 SC.reconfigure SCloose @11:49:21.36 Elbow.reconfigure TightElbow @11:49:21.41 Elbow.seek 2 @11:49:23.25 Forearm.reconfigure TightForearm @11:49:23.30 Forearm.seek 2 + 800 counts @11:49:24.45 SE.reconfigure SEpick @11:49:24.52 SE.seek beamBreak @11:49:26.80 SE.configure SEconfig @11:49:26.94 Elbow.seek 2 @11:49:27.10 Forearm.seek 2 + 300 counts @11:49:27.32 Forearm.reconfigure LooseForearm @11:49:27.37 Hand.grip @11:49:28.17 SE.seek down @11:49:28.33
Forearm.seek retract @11:49:29.30 Elbow.seek CC @11:49:30.25 SE.coast @11:49:30.85
Elbow.reconfigure LooseElbow @11:49:30.94 Forearm.reconfigure TightForearm @11:49:30.98 Forearm.seek CC @11:49:32.11 CC.seek closed @11:49:35.87 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.99 @11:49:35.88 Hand.release @11:49:36.70 Forearm.reconfigure LooseForearm @11:49:36.75 Forearm.seek retract @11:49:37.71 SC.coast @11:49:37.75 CTV.dial CSV,avoiding => intake @11:49:39.07 CSV.dial CTV @11:49:39.60 CS.seek 5.000ml @11:49:53.10 delay 1:20 @11:51:13.11 CS.seek 2.500ml @11:51:20.32 delay 40 seconds @11:52:00.33 CS.seek 0.000ml @11:52:07.63 delay 1:00 @11:53:07.65 PSV.dial :air @11:53:08.12 PS.seek 2.500ml @11:53:34.45 PSV.dial :mixloop @11:53:35.03 delay 3 seconds @11:53:38.05 PSV.dial :air @11:53:38.50 delay 2 seconds @11:53:40.51 PSV.dial :mixloop @11:53:40.97 delay 3 seconds @11:53:43.99 PSV.dial :waste1 @11:53:45.29 PS.seek empty @11:54:11.58 CBV.dial :waste1 @11:54:12.07 delay 5 seconds @11:54:17.08 CBV.dial AIV @11:54:17.55 delay 10 seconds @11:54:27.57 CBV.dial :waste1 @11:54:28.04 delay 10 seconds @11:54:38.05 CBV.dial AIV @11:54:38.51 delay 10 seconds @11:54:48.52 CTV.dial CSV,avoiding => intake @11:54:48.53 CBV.dial between AIV and waste1 @11:54:48.88 CSV.dial :air @11:54:49.33 CS.seek 10.000ml @11:55:16.07 CSV.dial CTV @11:55:16.59 CS.seek 0.000ml @11:55:42.72 CBV.dial :waste1 @11:55:43.03 delay 10 seconds @11:55:53.04 CTV.dial :air,avoiding => intake @11:55:54.40 Storing lyfil puck in Collection Clamp to tube 1 @11:55:54.55 SC.reconfigure SCtight @11:55:54.60 SC.seek 1 @11:55:56.78 SC.reconfigure SCloose @11:55:56.97 Elbow.reconfigure TightElbow @11:55:57.02 Elbow.seek 1 @11:55:58.63 Forearm.reconfigure TightForearm @11:55:58.68 Forearm.seek 1 + 800 counts @11:55:59.84 SE.reconfigure SEpick @11:55:59.91 SE.seek beamBreak @11:56:02.82 SE.configure SEconfig @11:56:02.95 Elbow.reconfigure LimpElbow @11:56:03.00 Forearm.reconfigure LooseForearm @11:56:03.05 Forearm.seek 1 @11:56:03.31 Elbow.hold @11:56:03.37 Elbow.reconfigure TightElbow @11:56:03.46 SE.seek 0.04 Pucks below Top Plate @11:56:03.76 Forearm.coast @11:56:03.79 Forearm.seek 1 + 347 counts @11:56:04.10 Forearm.reconfigure TightForearm @11:56:04.15 Forearm.seek 1 + 800 counts @11:56:04.34 SE.seek 1.04 Pucks below Top Plate @11:56:04.93 Forearm.reconfigure LooseForearm @11:56:04.98 Forearm.seek retract @11:56:05.91 Elbow.seek CC @11:56:07.49 Elbow.reconfigure LooseElbow @11:56:07.59 Forearm.seek CC @11:56:08.57 CC.seek sealed @11:56:09.50 Hand.grip @11:56:10.27 CC.seek open @11:56:13.64 Forearm.seek retract @11:56:14.58 Elbow.reconfigure TightElbow @11:56:14.63 Elbow.seek 1 @11:56:16.21 Elbow.reconfigure LimpElbow @11:56:16.27 Forearm.seek 1 @11:56:17.31 Elbow.hold @11:56:17.37 Elbow.reconfigure TightElbow @11:56:17.42 Hand.release @11:56:18.23 Forearm.coast @11:56:18.27 Forearm.seek 1 + 332 counts @11:56:18.52 Forearm.reconfigure TightForearm @11:56:18.57 Forearm.seek 1 + 800 counts @11:56:18.86 SE.seek down @11:56:19.96
Forearm.reconfigure LooseForearm @11:56:20.01 Forearm.seek retract @11:56:21.53 SE.coast @11:56:21.57
SC.coast @11:56:21.61 PSV.dial :air @11:56:22.68 PS.seek 0.499ml @11:56:28.66 delay 3 seconds @11:56:31.67 PSV.dial :mixloop @11:56:32.12 PRV.dial :air @11:56:32.58 delay 3 seconds @11:56:35.59 PRV.dial CBV @11:56:36.05 CBV.dial AIV @11:56:36.52 PRV.dial between CBV and air @11:56:36.85 PRV.dial :air @11:56:37.21 PS.reconfigure PSslow @11:56:37.26 PS.seek 0.599ml @11:56:39.76 PRV.dial :waste2 @11:56:40.50 PS.reconfigure PSconfig @11:56:40.54 PS.seek empty @11:56:47.47 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @11:56:47.64 Elbow.seek FlushPuck::Garage @11:56:49.43 Elbow.reconfigure LooseElbow @11:56:49.53 Forearm.seek garage @11:56:50.59 Hand.grip @11:56:51.49 Forearm.seek retract @11:56:53.12 Elbow.reconfigure TightElbow @11:56:53.17 Elbow.seek CC @11:56:55.95 Elbow.reconfigure LooseElbow @11:56:56.04 Forearm.reconfigure TightForearm @11:56:56.09 Forearm.seek CC @11:56:57.25 CC.seek closed @11:57:01.06 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.96 @11:57:01.13 Hand.release @11:57:01.94 Forearm.reconfigure LooseForearm @11:57:01.99 Forearm.seek retract @11:57:03.03 Clearing PML for modified lysate @11:57:03.04 CTV.dial :air,avoiding => intake @11:57:03.05 PRV.dial :waste2 @11:57:03.07 PTV.dial :PRV,avoiding => puck @11:57:03.81 delay 2 seconds @11:57:05.81 PTV.dial :PRVmixing,avoiding => puck @11:57:06.55 delay 2 seconds @11:57:08.56 PSV.dial :PML @11:57:08.57 delay 2 seconds @11:57:10.58 PRV.dial :air @11:57:11.33 delay 2 seconds @11:57:13.39 PS.seek 1.499ml @11:57:29.44 delay 5 seconds @11:57:34.45 PS.seek 2.500ml @11:57:45.50 PSV.dial :air @11:57:45.97 PS.seek 0.000ml @11:58:12.16 mixing lysate with speDil @11:58:12.17 PSV.dial :PML @11:58:12.75 CBV.dial :PRV @11:58:12.76 PRV.dial :air @11:58:12.77 delay 2 seconds @11:58:14.78 PRV.dial :speDil @11:58:15.24 delay 2 seconds @11:58:17.30 PS.reconfigure PSslow1 @11:58:17.35 PS.seek 0.225ml @11:58:40.69 delay 5 seconds @11:58:45.70 PRV.dial CBV @11:58:46.45 delay 2 seconds @11:58:48.51 PS.seek 0.475ml @11:59:14.28 delay 5 seconds @11:59:19.29 PRV.dial :air @11:59:19.77 delay 2 seconds @11:59:21.78 PS.seek 1.000ml @12:00:15.44 delay 10 seconds @12:00:25.45 PRV.dial CBV @12:00:25.92 CTV.dial :air,avoiding => intake @12:00:25.93 PSV.dial :air @12:00:26.51 PS.reconfigure PSconfig @12:00:26.55 PS.seek 2.500ml @12:00:42.78 delay 3 seconds @12:00:45.79 PSV.dial PTV @12:00:46.53 PTV.dial :PRV,avoiding => puck @12:00:47.29 PS.seek 0.000ml @12:01:13.59 PSV.dial :air @12:01:14.41 PS.seek 2.500ml @12:01:40.74 delay 3 seconds @12:01:43.75 PSV.dial PTV @12:01:44.60 PTV.dial :PRV,avoiding => puck @12:01:44.62 PS.seek 0.000ml @12:02:10.93 Pushing modified lysate into AIV Injection Loop @12:02:10.94 CBV.dial AIV @12:02:10.96 AIV.dial :bypass @12:02:10.98 PRV.dial CBV @12:02:10.99 PSV.dial :air @12:02:11.75 PS.seek 2.500ml @12:02:37.99 delay 3 seconds @12:02:41.01 PSV.dial :PML @12:02:41.48 PTV.dial :PRVmixing,avoiding => puck @12:02:42.28 PS.reconfigure PSslow1 @12:02:42.33 PS.seek 1.740ml @12:03:59.89 delay 5 seconds @12:04:04.90 AIV.dial :sample @12:04:05.45 AWV.select :waste @12:04:05.61 PS.reconfigure PSslow2 @12:04:05.65 PS.seek 1.220ml @12:05:51.58 delay 15 seconds @12:06:06.59 AIV.dial :bypass @12:06:07.15 PS.reconfigure PSconfig @12:06:07.19 PS.seek 0.000ml @12:06:20.48 PSV.dial :air @12:06:20.96 PS.seek 2.500ml @12:06:47.17 PSV.dial :PML @12:06:47.76 PS.seek 0.000ml @12:07:14.01 AIV.dial :coilToMFB @12:07:14.02 AWV.select :air @12:07:14.18 Extracting Nucleic Acid from AIV @12:07:14.20
Beginning samplePassD @12:07:14.23 SPE.hold 2:46:40,55.0C @12:07:14.26 ASV.dial :SPEdelivery @12:07:14.38
AWV.select :air @12:07:14.39 AIV.dial :bypass @12:07:14.41 PSV.dial PSR @12:07:14.65 delay 20 seconds @12:07:16.14
PSR.select :killMFB @12:07:16.26 PS.seek 1.500ml @12:07:32.39 delay 5 seconds @12:07:34.66 primeLysateINT @12:07:34.67 AWV.select :air @12:07:34.68 AIV.dial :coilToMFB @12:07:34.69 ATV.dial :AIV @12:07:36.03 AS.reconfigure ASslow1 @12:07:36.08 AS.seek 0.241ml @12:07:37.40
PSV.dial :waste1 @12:07:37.90 PS.seek 0.000ml @12:07:54.01 PSV.dial PSR @12:07:54.49 PSR.select :air @12:07:54.61 PS.seek 2.500ml @12:08:20.88 PRV.dial CBV @12:08:20.90
CBV.dial PRV @12:08:20.92 PSV.dial :mixloop @12:08:22.12 CTV.dial :air,avoiding => intake @12:08:22.13 PTV.dial :PRVmixing @12:08:22.16 PS.seek 0.000ml @12:08:25.70 delay 10 seconds @12:08:35.71 ATV.dial :air2 @12:08:36.29 AS.seek 0.271ml @12:08:42.67 ATV.dial :waste2 @12:08:43.14 AS.reconfigure ASnorm @12:08:43.19 AS.seek empty @12:08:48.38
PSV.dial PSR @12:08:49.45 PSR.select :flush @12:08:49.58 PS.seek 1.500ml @12:08:57.49 Lysate primed! @12:08:57.50 AFV.select :holdingCoil @12:08:57.51 ATV.dial :air2 @12:08:58.03 AS.reconfigure ASslow1 @12:08:58.08 AS.seek 0.069ml @12:09:05.63
delay 5 seconds @12:09:10.64 PSV.dial :waste1 @12:09:11.14 PS.seek 0.000ml @12:09:12.68 delay 3 seconds @12:09:15.68 ATV.dial :AIV @12:09:16.26 AS.seek 0.469ml @12:09:27.20
PSV.dial PSR @12:09:27.67 PSR.select :air @12:09:27.79 PS.seek 2.500ml @12:09:54.11 PRV.dial CBV @12:09:54.13
CBV.dial PRV @12:09:54.14 PSV.dial :mixloop @12:09:55.33 CTV.dial :air,avoiding => intake @12:09:55.34 PTV.dial :PRVmixing @12:09:55.37 PS.seek 0.000ml @12:10:21.83 PSV.dial PSR @12:10:22.86 PSR.select :flush @12:10:22.97 PS.seek 1.500ml @12:10:38.09 delay 5 seconds @12:10:39.01
delay 5 seconds @12:10:43.14 Solid Phase Extraction Heater at 52.4C @12:10:43.15 ATV.dial ASV @12:10:43.50 ASV.dial :SPEdelivery @12:10:43.58 AS.seek 0.041ml @12:10:44.02
PSV.dial :waste1 @12:10:44.48 PS.seek 0.000ml @12:11:00.48 PSV.dial PSR @12:11:00.95 PSR.select :air @12:11:01.07 PS.seek 2.500ml @12:11:27.39 PRV.dial CBV @12:11:27.41
CBV.dial PRV @12:11:27.43 PSV.dial :mixloop @12:11:28.48 CTV.dial :air,avoiding => intake @12:11:28.49 PTV.dial :PRVmixing @12:11:28.52 PS.seek 0.000ml @12:11:54.82 PSV.dial PSR @12:11:55.98 PSR.select :flush @12:11:56.09 PS.seek 1.500ml @12:12:11.14 delay 10 seconds @12:12:12.23
delay 5 seconds @12:12:17.24 PSV.dial :waste1 @12:12:17.85 PS.seek 0.000ml @12:12:21.15 rinseLysateLineINT @12:12:21.16 AFV.select :carrierSolution @12:12:21.27 delay 2 seconds @12:12:23.28 AS.reconfigure ASconfig @12:12:23.33 AS.seek 1.000ml @12:12:33.98
PSV.dial PSR @12:12:34.43 PSR.select :air @12:12:34.55 PS.seek 2.500ml @12:13:00.79 PRV.dial CBV @12:13:00.81
CBV.dial PRV @12:13:00.83 PSV.dial :mixloop @12:13:01.98 CTV.dial :air,avoiding => intake @12:13:01.99 PTV.dial :PRVmixing @12:13:02.02 PS.reconfigure PSslow @12:13:02.07 PS.seek 1.200ml @12:13:19.53 delay 1 second @12:13:20.54 AFV.select :holdingCoil @12:13:20.64 delay 1 second @12:13:21.65 ATV.dial :AIV @12:13:21.99 AIV.dial :coilToMFB @12:13:22.00 AWV.select :waste @12:13:22.11 AS.reconfigure ASslow0 @12:13:22.16 AS.seek 0.000ml @12:13:28.98
PSV.dial :waste1 @12:13:30.28 PS.reconfigure PSconfig @12:13:30.32 PS.seek 0.000ml @12:13:43.32 PSV.dial :air @12:13:44.43 PS.seek 2.500ml @12:14:10.75 PSV.dial :mixloop @12:14:11.20 PS.reconfigure PSslow @12:14:11.24 PS.seek 0.000ml @12:15:02.63 PSV.dial :air @12:15:03.09 PS.reconfigure PSconfig @12:15:03.13 PS.seek 2.500ml @12:15:04.49 ATV.dial :air2 @12:15:05.65 AS.reconfigure ASslow1 @12:15:05.70 AS.seek 0.050ml @12:15:16.19 ATV.dial :AIV @12:15:16.76 AS.seek 0.010ml @12:15:25.19 ATV.dial :waste @12:15:26.48 AS.seek empty @12:15:28.91 ethanol wash @12:15:28.92 ATV.dial :air2 @12:15:29.41
PSV.dial :mixloop @12:15:29.87 PS.reconfigure PSslow @12:15:29.93 PS.seek 0.000ml @12:15:30.03 AS.reconfigure ASslow2 @12:15:30.08 AS.seek 0.009ml @12:15:40.58 delay 3 seconds @12:15:43.59 Pulling 0.4ml of Ethanol Wash @12:15:43.60 ATV.dial :ethanol @12:15:44.18 AS.reconfigure ASslow1 @12:15:44.23 AS.seek 0.409ml @12:16:21.25
Cleaning Collection Syringe @12:16:21.26 CSV.dial CSR @12:16:21.70 CSR.select :flush @12:16:21.81 CS.seek 4.000ml @12:16:32.83 delay 5 seconds @12:16:37.84 CSR.select :air @12:16:37.94 CS.seek 10.000ml @12:16:54.02 CSV.dial CTV @12:16:54.06
CTV.dial CSV @12:16:55.37 CBV.dial :waste1 @12:16:55.87 CS.seek 0.000ml @12:17:06.10 delay 3 seconds @12:17:09.11 ATV.dial :air1 @12:17:09.46 delay 3 seconds @12:17:12.52 AS.seek 0.434ml @12:17:17.88 delay 3 seconds @12:17:20.89 ATV.dial :ethanol @12:17:21.27 AS.seek 0.419ml @12:17:21.98
CSV.dial CSR @12:17:22.50 CSR.select :flush @12:17:22.61 CS.seek 4.000ml @12:17:24.58 delay 3 seconds @12:17:27.59 ATV.dial ASV @12:17:28.82 AS.seek 0.092ml @12:17:33.54
delay 5 seconds @12:17:38.55 CSR.select :air @12:17:38.65 CS.seek 10.000ml @12:17:54.73 CSV.dial CTV @12:17:54.75
CTV.dial CSV @12:17:55.29 CBV.dial :waste1 @12:17:55.30 CS.seek 0.000ml @12:18:21.50 Garaging FlushPuck in Collection Clamp @12:18:21.56 Elbow.reconfigure TightElbow @12:18:21.61 Elbow.seek CC @12:18:21.73 Elbow.reconfigure LooseElbow @12:18:21.82 Forearm.seek CC @12:18:22.78 CC.seek sealed @12:18:23.73 Hand.grip @12:18:24.47 CC.seek open @12:18:27.80 Forearm.seek retract @12:18:28.76 Elbow.reconfigure TightElbow @12:18:28.81 Elbow.seek garage @12:18:31.62 Elbow.reconfigure LooseElbow @12:18:31.71 Forearm.reconfigure TightForearm @12:18:31.76 Forearm.seek garage @12:18:32.95 Hand.release @12:18:33.76 Forearm.reconfigure LooseForearm @12:18:33.81 Forearm.seek retract @12:18:34.86 Whole Cell Archive sampling 1000ml @12:18:34.87 Loading wcr puck from tube 2 into Collection Clamp @12:18:35.07 SC.reconfigure SCtight @12:18:35.12 SC.seek 2 @12:18:35.77 delay 3 seconds @12:18:37.39 SC.reconfigure SCloose @12:18:37.58 Elbow.reconfigure TightElbow @12:18:37.63 Elbow.seek 2 @12:18:38.78 ATV.dial :waste2 @12:18:39.44 AS.reconfigure ASconfig @12:18:39.50 AS.seek empty @12:18:39.60 Forearm.reconfigure TightForearm @12:18:39.65 Forearm.seek 2 + 800 counts @12:18:40.68 SE.reconfigure SEpick @12:18:40.75 SE.seek beamBreak @12:18:43.12 SE.configure SEconfig @12:18:43.26 Elbow.seek 2 @12:18:43.42 Forearm.seek 2 + 300 counts @12:18:44.27 Forearm.reconfigure LooseForearm @12:18:44.32 Hand.grip @12:18:45.12 SE.seek down @12:18:45.23 ATV.dial :air2 @12:18:45.33 Forearm.seek retract @12:18:45.62 AS.reconfigure ASslow1 @12:18:45.66 AS.seek 1.000ml @12:18:46.35 Elbow.seek CC @12:18:47.42 SE.coast @12:18:47.85 Elbow.reconfigure LooseElbow @12:18:47.95 Forearm.reconfigure TightForearm @12:18:48.00 Forearm.seek CC @12:18:49.15 CC.seek closed @12:18:53.00 Collection Clamp onSpring/offSpring for 3/21 samples, on/off torque 1.99 @12:18:53.01 Hand.release @12:18:53.76 Forearm.reconfigure LooseForearm @12:18:53.81 Forearm.seek retract @12:18:54.79 SC.coast @12:18:54.83 collectSample 1000ml w/bubble point of 55psi @12:18:54.85 Priming 7ml @12:18:54.87 Intake.select :close @12:18:54.88 Exhaust.select :close @12:18:54.90 SS.hold @12:18:55.08 Calibrating pressure sensors @12:18:55.09 CTV.dial :air @12:18:56.44 SSV.dial :spit,avoiding => exhaust @12:18:56.45 SS.reconfigure SSfast @12:18:56.52 SS.seek 5.00ml @12:18:59.21 SSV.dial CBV @12:18:59.23 CBV.dial SSV @12:19:00.61 delay 3.8 seconds @12:19:04.50 SSV.dial :spit @12:19:05.81 SS.seek 0.00ml @12:19:08.64 SSV.dial CBV @12:19:09.97 CTV.dial :intake @12:19:10.75 SSV.dial CBV @12:19:10.76 CBV.dial SSV @12:19:10.78 Allowing 55s for 7.0ml of remaining 7.0ml @12:19:10.80 Intake.select :open @12:19:12.96 Intake @37.9psia, vacuum from 20.0 to 24.0psi @12:19:12.98 SS.reconfigure forSampling @12:19:13.04 SS.seek 7.00ml @12:19:16.51 Vacuum too high -- waiting up to 40s @12:19:17.21 Intake @34.9psia, vacuum from 20.0 to 24.0psi @12:19:17.22 SS.reconfigure forSampling @12:19:17.28 SS.seek 7.00ml @12:19:20.83 Stroke completed -- waiting for pressure to equalize ... @12:19:23.82 Intake.select :close @12:19:23.98 SSV.dial :exhaust @12:19:24.75 Exhaust.select :open @12:19:24.86 SS.reconfigure SSexhale @12:19:24.91 SS.seek 0.00ml @12:19:28.56 Exhaust.select :close @12:19:28.66 Sampling 1000.0ml @12:19:28.67 Intake.select :close @12:19:28.68 Exhaust.select :close @12:19:28.69 SS.reconfigure SSconfig @12:19:28.75 SS.hold @12:19:28.92 CTV.dial :intake @12:19:28.93 SSV.dial CBV @12:19:28.97 CBV.dial SSV @12:19:29.74 Allowing 185s for 25.0ml of remaining 1000.0ml @12:19:29.75 Intake.select :open @12:19:31.91 Intake @37.8psia, vacuum from 20.0 to 24.0psi @12:19:31.93 SS.reconfigure forSampling @12:19:31.99 SS.seek 25.00ml @12:19:34.79 Vacuum too high -- waiting up to 40s @12:19:35.07 Intake @33.9psia, vacuum from 20.0 to 24.0psi @12:19:35.08 SS.reconfigure forSampling @12:19:35.14 SS.seek 25.00ml @12:19:37.85 Vacuum too high -- waiting up to 40s @12:19:38.34 Intake @34.2psia, vacuum from 20.0 to 24.0psi @12:19:38.35 SS.reconfigure forSampling @12:19:38.42 SS.seek 25.00ml @12:19:51.13 Vacuum too high -- waiting up to 40s @12:19:52.18 Intake @37.4psia, vacuum from 20.0 to 24.0psi @12:19:52.19 SS.reconfigure forSampling @12:19:52.26 SS.seek 25.00ml @12:19:54.53 Stroke completed -- waiting for pressure to equalize ... @12:19:55.17 Intake.select :close @12:19:55.33 SSV.dial :exhaust @12:19:56.12 Exhaust.select :open @12:19:56.24 SS.reconfigure SSexhale @12:19:56.30 SS.seek 0.00ml @12:20:05.23 Exhaust.select :close @12:20:05.33 SSV.dial CBV @12:20:05.35 CBV.dial SSV @12:20:06.12 Allowing 185s for 25.0ml of remaining 975.0ml @12:20:06.14 Intake.select :open @12:20:08.29 Intake @37.5psia, vacuum from 20.0 to 24.0psi @12:20:08.31 SS.reconfigure forSampling @12:20:08.37 SS.seek 25.00ml @12:20:29.39 Stroke completed -- waiting for pressure to equalize ... @12:20:30.03 Intake.select :close @12:20:30.17 SSV.dial :exhaust @12:20:30.95 Exhaust.select :open @12:20:31.07 SS.reconfigure SSexhale @12:20:31.12 SS.seek 0.00ml @12:20:40.39 Exhaust.select :close @12:20:40.49 SSV.dial CBV @12:20:40.51 CBV.dial SSV @12:20:41.28 Allowing 185s for 25.0ml of remaining 950.0ml @12:20:41.30 Intake.select :open @12:20:43.45 Intake @37.1psia, vacuum from 20.0 to 24.0psi @12:20:43.47 SS.reconfigure forSampling @12:20:43.53 SS.seek 25.00ml @12:21:04.98 Stroke completed -- waiting for pressure to equalize ... @12:21:05.62 Intake.select :close @12:21:05.77 SSV.dial :exhaust @12:21:06.54 Exhaust.select :open @12:21:06.66 SS.reconfigure SSexhale @12:21:06.71 SS.seek 0.00ml @12:21:15.45 Exhaust.select :close @12:21:15.55 SSV.dial CBV @12:21:15.57 CBV.dial SSV @12:21:16.35 Allowing 185s for 25.0ml of remaining 925.0ml @12:21:16.37 Intake.select :open @12:21:18.52 Intake @36.8psia, vacuum from 20.0 to 24.0psi @12:21:18.54 SS.reconfigure forSampling @12:21:18.60 SS.seek 25.00ml @12:21:39.59 Stroke completed -- waiting for pressure to equalize ... @12:21:40.23 Intake.select :close @12:21:40.37 SSV.dial :exhaust @12:21:41.15 Exhaust.select :open @12:21:41.26 SS.reconfigure SSexhale @12:21:41.31 SS.seek 0.00ml @12:21:50.45 Exhaust.select :close @12:21:50.56 SSV.dial CBV @12:21:50.58 CBV.dial SSV @12:21:51.35 Allowing 185s for 25.0ml of remaining 900.0ml @12:21:51.37 Intake.select :open @12:21:53.53 Intake @36.4psia, vacuum from 20.0 to 24.0psi @12:21:53.55 SS.reconfigure forSampling @12:21:53.61 SS.seek 25.00ml @12:22:10.09 delay 5 seconds @12:22:15.10 ATV.dial ASV @12:22:15.44 Stroke completed -- waiting for pressure to equalize ... @12:22:15.82 ASV.dial :SPEdelivery @12:22:15.84 AS.seek 0.020ml @12:22:16.08 Intake.select :close @12:22:16.23 SSV.dial :exhaust @12:22:17.01 Exhaust.select :open @12:22:17.13 SS.reconfigure SSexhale @12:22:17.18 SS.seek 0.00ml @12:22:25.93 Exhaust.select :close @12:22:26.04 SSV.dial CBV @12:22:26.06 CBV.dial SSV @12:22:26.85 Allowing 185s for 25.0ml of remaining 875.0ml @12:22:26.87 Intake.select :open @12:22:29.02 Intake @36.1psia, vacuum from 20.0 to 24.0psi @12:22:29.04 SS.reconfigure forSampling @12:22:29.10 SS.seek 25.00ml @12:22:50.97 Stroke completed -- waiting for pressure to equalize ... @12:22:51.61 Intake.select :close @12:22:51.75 SSV.dial :exhaust @12:22:52.57 Exhaust.select :open @12:22:52.69 SS.reconfigure SSexhale @12:22:52.74 SS.seek 0.00ml @12:23:01.43 Exhaust.select :close @12:23:01.54 SSV.dial CBV @12:23:01.56 CBV.dial SSV @12:23:02.33 Allowing 185s for 25.0ml of remaining 850.0ml @12:23:02.35 Intake.select :open @12:23:04.50 Intake @36.1psia, vacuum from 20.0 to 24.0psi @12:23:04.52 SS.reconfigure forSampling @12:23:04.58 SS.seek 25.00ml @12:23:18.86 Vacuum too high -- waiting up to 40s @12:23:19.13 Intake @31.9psia, vacuum from 20.0 to 24.0psi @12:23:19.15 SS.reconfigure forSampling @12:23:19.21 SS.seek 25.00ml @12:23:27.57 Stroke completed -- waiting for pressure to equalize ... @12:23:28.21 Intake.select :close @12:23:28.35 SSV.dial :exhaust @12:23:29.13 Exhaust.select :open @12:23:29.25 SS.reconfigure SSexhale @12:23:29.82 SS.seek 0.00ml @12:23:38.67 Exhaust.select :close @12:23:38.77 SSV.dial CBV @12:23:38.79 CBV.dial SSV @12:23:39.57 Allowing 185s for 25.0ml of remaining 825.0ml @12:23:39.58 Intake.select :open @12:23:41.73 Intake @35.6psia, vacuum from 20.0 to 24.0psi @12:23:41.75 SS.reconfigure forSampling @12:23:41.81 SS.seek 25.00ml @12:24:06.63 Stroke completed -- waiting for pressure to equalize ... @12:24:07.27 Intake.select :close @12:24:07.41 SSV.dial :exhaust @12:24:08.20 Exhaust.select :open @12:24:08.31 SS.reconfigure SSexhale @12:24:08.36 SS.seek 0.00ml @12:24:17.67 Exhaust.select :close @12:24:17.77 SSV.dial CBV @12:24:17.79 CBV.dial SSV @12:24:18.56 Allowing 185s for 25.0ml of remaining 800.0ml @12:24:18.58 Intake.select :open @12:24:20.72 Intake @35.4psia, vacuum from 20.0 to 24.0psi @12:24:20.74 SS.reconfigure forSampling @12:24:20.80 SS.seek 25.00ml @12:24:45.81 Stroke completed -- waiting for pressure to equalize ... @12:24:46.45 Intake.select :close @12:24:46.59 SSV.dial :exhaust @12:24:47.36 Exhaust.select :open @12:24:47.47 SS.reconfigure SSexhale @12:24:47.52 SS.seek 0.00ml @12:24:56.40 Exhaust.select :close @12:24:56.50 SSV.dial CBV @12:24:56.52 CBV.dial SSV @12:24:57.29 Allowing 185s for 25.0ml of remaining 775.0ml @12:24:57.31 Intake.select :open @12:24:59.46 Intake @35.1psia, vacuum from 20.0 to 24.0psi @12:24:59.48 SS.reconfigure forSampling @12:24:59.54 SS.seek 25.00ml @12:25:24.36 Stroke completed -- waiting for pressure to equalize ... @12:25:25.00 Intake.select :close @12:25:25.15 SSV.dial :exhaust @12:25:25.92 Exhaust.select :open @12:25:26.03 SS.reconfigure SSexhale @12:25:26.08 SS.seek 0.00ml @12:25:34.90 Exhaust.select :close @12:25:35.01 SSV.dial CBV @12:25:35.03 CBV.dial SSV @12:25:35.82 Allowing 185s for 25.0ml of remaining 750.0ml @12:25:35.84 Intake.select :open @12:25:36.05 delay 5 seconds @12:25:37.99 Intake @34.8psia, vacuum from 20.0 to 24.0psi @12:25:38.01 SS.reconfigure forSampling @12:25:38.07 SS.seek 25.00ml @12:25:41.06 ATV.dial :air2 @12:25:41.83 AS.seek 0.520ml @12:26:03.51 Stroke completed -- waiting for pressure to equalize ... @12:26:04.15 Intake.select :close @12:26:04.31 SSV.dial :exhaust @12:26:05.09 Exhaust.select :open @12:26:05.21 SS.reconfigure SSexhale @12:26:05.26 SS.seek 0.00ml @12:26:14.49 Exhaust.select :close @12:26:14.59 SSV.dial CBV @12:26:14.61 CBV.dial SSV @12:26:15.38 Allowing 185s for 25.0ml of remaining 725.0ml @12:26:15.40 Intake.select :open @12:26:17.55 Intake @34.6psia, vacuum from 20.0 to 24.0psi @12:26:17.57 SS.reconfigure forSampling @12:26:17.63 SS.seek 25.00ml @12:26:42.27 Stroke completed -- waiting for pressure to equalize ... @12:26:42.91 Intake.select :close @12:26:43.05 SSV.dial :exhaust @12:26:43.81 Exhaust.select :open @12:26:43.91 SS.reconfigure SSexhale @12:26:43.96 SS.seek 0.00ml @12:26:52.67 Exhaust.select :close @12:26:52.77 SSV.dial CBV @12:26:52.79 CBV.dial SSV @12:26:53.57 Allowing 185s for 25.0ml of remaining 700.0ml @12:26:53.58 Intake.select :open @12:26:55.72 Intake @34.5psia, vacuum from 20.0 to 24.0psi @12:26:55.74 SS.reconfigure forSampling @12:26:55.80 SS.seek 25.00ml @12:27:20.56 Stroke completed -- waiting for pressure to equalize ... @12:27:21.20 Intake.select :close @12:27:21.34 SSV.dial :exhaust @12:27:22.13 Exhaust.select :open @12:27:22.23 SS.reconfigure SSexhale @12:27:22.28 SS.seek 0.00ml @12:27:24.10 delay 5 seconds @12:27:29.11 ATV.dial ASV @12:27:29.83 ASV.dial :wasteR @12:27:30.17 AS.seek 0.300ml @12:27:30.87 Exhaust.select :close @12:27:30.97 SSV.dial CBV @12:27:30.99 CBV.dial SSV @12:27:31.78 Allowing 185s for 25.0ml of remaining 675.0ml @12:27:31.79 Intake.select :open @12:27:33.93 Intake @34.2psia, vacuum from 20.0 to 24.0psi @12:27:33.95 SS.reconfigure forSampling @12:27:34.01 SS.seek 25.00ml @12:27:58.86 Stroke completed -- waiting for pressure to equalize ... @12:28:00.01 Intake.select :close @12:28:00.15 SSV.dial :exhaust @12:28:00.92 Exhaust.select :open @12:28:01.03 SS.reconfigure SSexhale @12:28:01.09 SS.seek 0.00ml @12:28:10.19 Exhaust.select :close @12:28:10.29 SSV.dial CBV @12:28:10.31 CBV.dial SSV @12:28:11.08 Allowing 185s for 25.0ml of remaining 650.0ml @12:28:11.10 Intake.select :open @12:28:13.26 Intake @33.9psia, vacuum from 20.0 to 24.0psi @12:28:13.28 SS.reconfigure forSampling @12:28:13.34 SS.seek 25.00ml @12:28:15.30 delay 20 seconds @12:28:35.31 AS.seek 0.070ml @12:28:38.04 Stroke completed -- waiting for pressure to equalize ... @12:28:38.68 Intake.select :close @12:28:38.83 SSV.dial :exhaust @12:28:39.62 Exhaust.select :open @12:28:39.74 SS.reconfigure SSexhale @12:28:39.79 SS.seek 0.00ml @12:28:48.43 Exhaust.select :close @12:28:48.54 SSV.dial CBV @12:28:48.56 CBV.dial SSV @12:28:49.58 Allowing 185s for 25.0ml of remaining 625.0ml @12:28:49.59 Intake.select :open @12:28:51.74 Intake @33.7psia, vacuum from 20.0 to 24.0psi @12:28:51.76 SS.reconfigure forSampling @12:28:51.82 SS.seek 25.00ml @12:29:16.67 Stroke completed -- waiting for pressure to equalize ... @12:29:17.31 Intake.select :close @12:29:17.45 SSV.dial :exhaust @12:29:18.23 Exhaust.select :open @12:29:18.35 SS.reconfigure SSexhale @12:29:18.40 SS.seek 0.00ml @12:29:22.50 delay 20 seconds @12:29:27.06 Exhaust.select :close @12:29:27.16 SSV.dial CBV @12:29:27.18 CBV.dial SSV @12:29:27.95 Allowing 185s for 25.0ml of remaining 600.0ml @12:29:27.97 Intake.select :open @12:29:30.12 Intake @33.5psia, vacuum from 20.0 to 24.0psi @12:29:30.14 SS.reconfigure forSampling @12:29:30.20 SS.seek 25.00ml @12:29:42.51 ATV.dial :waste2 @12:29:43.15 AS.reconfigure ASconfig @12:29:43.19 AS.seek empty @12:29:47.63 Elution @12:29:47.64 ATV.dial :air1 @12:29:48.71 AS.reconfigure ASslow1 @12:29:48.76 AS.seek 0.049ml @12:29:55.05 Stroke completed -- waiting for pressure to equalize ... @12:29:55.69 Intake.select :close @12:29:55.83 SSV.dial :exhaust @12:29:56.62 Exhaust.select :open @12:29:56.72 SS.reconfigure SSexhale @12:29:56.77 SS.seek 0.00ml @12:29:59.28 delay 3 seconds @12:30:02.29 ATV.dial :ElutionWater @12:30:02.96 AS.seek 0.109ml @12:30:05.37 Exhaust.select :close @12:30:05.48 SSV.dial CBV @12:30:05.50 CBV.dial SSV @12:30:06.29 Allowing 185s for 25.0ml of remaining 575.0ml @12:30:06.31 Intake.select :open @12:30:08.46 Intake @33.2psia, vacuum from 20.0 to 24.0psi @12:30:08.48 SS.reconfigure forSampling @12:30:08.54 SS.seek 25.00ml @12:30:15.49 delay 3 seconds @12:30:18.50 ATV.dial ASV,via => air2 @12:30:20.14 ASV.dial :SPEelution @12:30:20.92 AFV.select :carrierSolution @12:30:21.07 AS.reconfigure ASnorm @12:30:21.11 AS.seek 0.759ml @12:30:33.44 Stroke completed -- waiting for pressure to equalize ... @12:30:34.09 Intake.select :close @12:30:34.24 SSV.dial :exhaust @12:30:35.01 Exhaust.select :open @12:30:35.13 SS.reconfigure SSexhale @12:30:35.18 SS.seek 0.00ml @12:30:43.79 Exhaust.select :close @12:30:43.91 SSV.dial CBV @12:30:43.93 CBV.dial SSV @12:30:44.71 Allowing 185s for 25.0ml of remaining 550.0ml @12:30:44.73 Intake.select :open @12:30:46.89 Intake @33.0psia, vacuum from 20.0 to 24.0psi @12:30:46.91 SS.reconfigure forSampling @12:30:46.97 SS.seek 25.00ml @12:30:54.66 delay 3 seconds @12:30:57.67 AFV.select :holdingCoil @12:30:57.83 AS.reconfigure ASslow1 @12:30:57.87 AS.seek 0.489ml @12:31:11.83 Stroke completed -- waiting for pressure to equalize ... @12:31:12.47 Intake.select :close @12:31:12.62 SSV.dial :exhaust @12:31:13.39 Exhaust.select :open @12:31:13.49 SS.reconfigure SSexhale @12:31:13.54 SS.seek 0.00ml @12:31:22.19 Exhaust.select :close @12:31:22.29 SSV.dial CBV @12:31:22.31 CBV.dial SSV @12:31:23.08 Allowing 185s for 25.0ml of remaining 525.0ml @12:31:23.10 Intake.select :open @12:31:25.25 Intake @32.7psia, vacuum from 20.0 to 24.0psi @12:31:25.27 SS.reconfigure forSampling @12:31:25.33 SS.seek 25.00ml @12:31:50.50 Stroke completed -- waiting for pressure to equalize ... @12:31:51.73 Intake.select :close @12:31:51.88 SSV.dial :exhaust @12:31:52.67 Exhaust.select :open @12:31:52.79 SS.reconfigure SSexhale @12:31:52.84 SS.seek 0.00ml @12:31:53.18 delay 5 seconds @12:31:58.24 AS.reconfigure ASslow2 @12:31:58.29 AS.seek 0.454ml @12:32:01.58 Exhaust.select :close @12:32:01.68 SSV.dial CBV @12:32:01.70 CBV.dial SSV @12:32:02.47 Allowing 185s for 25.0ml of remaining 500.0ml @12:32:02.49 Intake.select :open @12:32:04.65 Intake @32.5psia, vacuum from 20.0 to 24.0psi @12:32:04.67 SS.reconfigure forSampling @12:32:04.73 SS.seek 25.00ml @12:32:29.59 Stroke completed -- waiting for pressure to equalize ... @12:32:30.82 Intake.select :close @12:32:30.96 SSV.dial :exhaust @12:32:31.73 Exhaust.select :open @12:32:31.84 SS.reconfigure SSexhale @12:32:31.89 SS.seek 0.00ml @12:32:34.34 delay 1:00 @12:32:40.48 Exhaust.select :close @12:32:40.58 SSV.dial CBV @12:32:40.60 CBV.dial SSV @12:32:41.37 Allowing 185s for 25.0ml of remaining 475.0ml @12:32:41.39 Intake.select :open @12:32:43.55 Intake @32.5psia, vacuum from 20.0 to 24.0psi @12:32:43.57 SS.reconfigure forSampling @12:32:43.63 SS.seek 25.00ml @12:33:08.45 Stroke completed -- waiting for pressure to equalize ... @12:33:09.68 Intake.select :close @12:33:09.83 SSV.dial :exhaust @12:33:10.62 Exhaust.select :open @12:33:10.74 SS.reconfigure SSexhale @12:33:10.79 SS.seek 0.00ml @12:33:19.39 Exhaust.select :close @12:33:19.49 SSV.dial CBV @12:33:19.51 CBV.dial SSV @12:33:20.28 Allowing 185s for 25.0ml of remaining 450.0ml @12:33:20.30 Intake.select :open @12:33:22.45 Intake @32.2psia, vacuum from 20.0 to 24.0psi @12:33:22.47 SS.reconfigure forSampling @12:33:22.53 SS.seek 25.00ml @12:33:34.35 pushing eluate to holding coil @12:33:34.41 AS.seek 0.294ml @12:33:47.34 Stroke completed -- waiting for pressure to equalize ... @12:33:48.57 Intake.select :close @12:33:48.72 SSV.dial :exhaust @12:33:49.49 Exhaust.select :open @12:33:49.61 SS.reconfigure SSexhale @12:33:49.66 SS.seek 0.00ml @12:33:58.26 Exhaust.select :close @12:33:58.37 SSV.dial CBV @12:33:58.39 CBV.dial SSV @12:33:59.17 Allowing 185s for 25.0ml of remaining 425.0ml @12:33:59.19 Intake.select :open @12:34:01.34 Intake @32.0psia, vacuum from 20.0 to 24.0psi @12:34:01.36 SS.reconfigure forSampling @12:34:01.42 SS.seek 25.00ml @12:34:26.20 Stroke completed -- waiting for pressure to equalize ... @12:34:27.43 Intake.select :close @12:34:27.58 SSV.dial :exhaust @12:34:28.37 Exhaust.select :open @12:34:28.47 SS.reconfigure SSexhale @12:34:28.52 SS.seek 0.00ml @12:34:37.15 Exhaust.select :close @12:34:37.26 SSV.dial CBV @12:34:37.28 CBV.dial SSV @12:34:38.07 Allowing 185s for 25.0ml of remaining 400.0ml @12:34:38.09 Intake.select :open @12:34:40.24 Intake @31.6psia, vacuum from 20.0 to 24.0psi @12:34:40.26 SS.reconfigure forSampling @12:34:40.32 SS.seek 25.00ml @12:35:05.13 Stroke completed -- waiting for pressure to equalize ... @12:35:06.36 Intake.select :close @12:35:06.50 SSV.dial :exhaust @12:35:07.27 Exhaust.select :open @12:35:07.37 SS.reconfigure SSexhale @12:35:07.42 SS.seek 0.00ml @12:35:16.04 Exhaust.select :close @12:35:16.15 SSV.dial CBV @12:35:16.17 CBV.dial SSV @12:35:16.96 Allowing 185s for 25.0ml of remaining 375.0ml @12:35:16.98 Intake.select :open @12:35:19.14 Intake @31.4psia, vacuum from 20.0 to 24.0psi @12:35:19.15 SS.reconfigure forSampling @12:35:19.21 SS.seek 25.00ml @12:35:44.12 Stroke completed -- waiting for pressure to equalize ... @12:35:45.33 Intake.select :close @12:35:45.49 SSV.dial :exhaust @12:35:46.80 Exhaust.select :open @12:35:46.93 SS.reconfigure SSexhale @12:35:46.98 SS.seek 0.00ml @12:35:55.74 Exhaust.select :close @12:35:55.84 SSV.dial CBV @12:35:55.86 CBV.dial SSV @12:35:56.64 Allowing 185s for 25.0ml of remaining 350.0ml @12:35:56.66 Intake.select :open @12:35:58.80 Intake @31.2psia, vacuum from 20.0 to 24.0psi @12:35:58.82 SS.reconfigure forSampling @12:35:58.88 SS.seek 25.00ml @12:36:17.77 delay 30 seconds @12:36:23.72 Stroke completed -- waiting for pressure to equalize ... @12:36:24.95 Intake.select :close @12:36:25.09 SSV.dial :exhaust @12:36:25.86 Exhaust.select :open @12:36:25.98 SS.reconfigure SSexhale @12:36:26.03 SS.seek 0.00ml @12:36:34.74 Exhaust.select :close @12:36:34.85 SSV.dial CBV @12:36:34.87 CBV.dial SSV @12:36:35.64 Allowing 185s for 25.0ml of remaining 325.0ml @12:36:35.66 Intake.select :open @12:36:37.82 Intake @31.0psia, vacuum from 20.0 to 24.0psi @12:36:37.84 SS.reconfigure forSampling @12:36:37.90 SS.seek 25.00ml @12:36:47.78 ATV.dial :waste,via => air3 @12:36:49.31 AS.reconfigure ASconfig @12:36:49.36 AS.seek empty @12:37:02.73 Stroke completed -- waiting for pressure to equalize ... @12:37:03.96 Intake.select :close @12:37:04.11 SSV.dial :exhaust @12:37:04.89 Exhaust.select :open @12:37:05.01 SS.reconfigure SSexhale @12:37:05.06 SS.seek 0.00ml @12:37:06.86 Parking Bleach in LysateSource line and SPEcolumn @12:37:06.87 ATV.dial :air3 @12:37:07.52 AS.reconfigure ASslow1 @12:37:07.57 AS.seek 0.029ml @12:37:14.00 ATV.dial :ElutionWater @12:37:14.16 Exhaust.select :close @12:37:14.26 SSV.dial CBV @12:37:14.28 CBV.dial SSV @12:37:14.40 AS.seek 0.059ml @12:37:15.06 Allowing 185s for 25.0ml of remaining 300.0ml @12:37:15.07 Intake.select :open @12:37:17.22 Intake @30.9psia, vacuum from 20.0 to 24.0psi @12:37:17.24 SS.reconfigure forSampling @12:37:17.30 SS.seek 25.00ml @12:37:20.77 ATV.dial :air3 @12:37:21.16 AS.seek 0.069ml @12:37:23.47 delay 2 seconds @12:37:25.48 ATV.dial :bleach @12:37:25.86 AS.seek 0.711ml @12:37:42.03 Stroke completed -- waiting for pressure to equalize ... @12:37:43.26 Intake.select :close @12:37:43.41 SSV.dial :exhaust @12:37:44.20 Exhaust.select :open @12:37:44.32 SS.reconfigure SSexhale @12:37:44.37 SS.seek 0.00ml @12:37:53.03 Exhaust.select :close @12:37:53.14 SSV.dial CBV @12:37:53.16 CBV.dial SSV @12:37:53.94 Allowing 185s for 25.0ml of remaining 275.0ml @12:37:53.95 Intake.select :open @12:37:56.10 Intake @30.5psia, vacuum from 20.0 to 24.0psi @12:37:56.12 SS.reconfigure forSampling @12:37:56.18 SS.seek 25.00ml @12:38:21.00 Stroke completed -- waiting for pressure to equalize ... @12:38:22.23 Intake.select :close @12:38:22.38 SSV.dial :exhaust @12:38:23.15 Exhaust.select :open @12:38:23.27 SS.reconfigure SSexhale @12:38:23.32 SS.seek 0.00ml @12:38:31.98 Exhaust.select :close @12:38:32.09 SSV.dial CBV @12:38:32.11 CBV.dial SSV @12:38:32.90 Allowing 185s for 25.0ml of remaining 250.0ml @12:38:32.91 Intake.select :open @12:38:35.07 Intake @30.3psia, vacuum from 20.0 to 24.0psi @12:38:35.09 SS.reconfigure forSampling @12:38:35.15 SS.seek 25.00ml @12:39:00.02 Stroke completed -- waiting for pressure to equalize ... @12:39:01.25 Intake.select :close @12:39:01.39 SSV.dial :exhaust @12:39:02.18 Exhaust.select :open @12:39:02.30 SS.reconfigure SSexhale @12:39:02.35 SS.seek 0.00ml @12:39:11.04 Exhaust.select :close @12:39:11.15 SSV.dial CBV @12:39:11.17 CBV.dial SSV @12:39:11.97 Allowing 185s for 25.0ml of remaining 225.0ml @12:39:11.98 Intake.select :open @12:39:14.15 Intake @30.3psia, vacuum from 20.0 to 24.0psi @12:39:14.16 SS.reconfigure forSampling @12:39:14.22 SS.seek 25.00ml @12:39:37.08 delay 3 seconds @12:39:39.51 Stroke completed -- waiting for pressure to equalize ... @12:39:40.15 Intake.select :close @12:39:40.30 SSV.dial :exhaust @12:39:40.61 ATV.dial :air3 @12:39:40.97 delay 3 seconds @12:39:41.07 Exhaust.select :open @12:39:41.18 SS.reconfigure SSexhale @12:39:41.23 SS.seek 0.00ml @12:39:44.03 AS.seek 0.736ml @12:39:49.37 delay 3 seconds @12:39:50.04 Exhaust.select :close @12:39:50.14 SSV.dial CBV @12:39:50.16 CBV.dial SSV @12:39:50.94 Allowing 185s for 25.0ml of remaining 200.0ml @12:39:50.95 Intake.select :open @12:39:52.38 ATV.dial :bleach @12:39:52.78 AS.seek 0.721ml @12:39:53.11 Intake @30.1psia, vacuum from 20.0 to 24.0psi @12:39:53.13 SS.reconfigure forSampling @12:39:53.19 SS.seek 25.00ml @12:39:56.11 delay 3 seconds @12:39:59.12 ATV.dial :AIV @12:40:00.21 AS.reconfigure ASconfig @12:40:00.25 AS.seek 0.015ml @12:40:18.05 Stroke completed -- waiting for pressure to equalize ... @12:40:19.29 Intake.select :close @12:40:19.44 SSV.dial :exhaust @12:40:20.21 Exhaust.select :open @12:40:20.33 SS.reconfigure SSexhale @12:40:20.38 SS.seek 0.00ml @12:40:29.10 Exhaust.select :close @12:40:29.20 SSV.dial CBV @12:40:29.22 CBV.dial SSV @12:40:29.99 Allowing 185s for 25.0ml of remaining 175.0ml @12:40:30.01 Intake.select :open @12:40:32.16 Intake @29.8psia, vacuum from 20.0 to 24.0psi @12:40:32.18 SS.reconfigure forSampling @12:40:32.24 SS.seek 25.00ml @12:40:41.70 ATV.dial :waste @12:40:42.97 AS.seek empty @12:40:44.15 ATV.dial :air3 @12:40:44.81 AS.reconfigure ASslow1 @12:40:44.86 AS.seek 0.009ml @12:40:47.18 delay 2 seconds @12:40:49.19 ATV.dial :bleach @12:40:49.58 AS.seek 0.579ml @12:40:57.10 Stroke completed -- waiting for pressure to equalize ... @12:40:58.33 Intake.select :close @12:40:58.48 SSV.dial :exhaust @12:40:59.25 Exhaust.select :open @12:40:59.36 SS.reconfigure SSexhale @12:40:59.41 SS.seek 0.00ml @12:41:08.08 Exhaust.select :close @12:41:08.18 SSV.dial CBV @12:41:08.20 CBV.dial SSV @12:41:08.99 Allowing 185s for 25.0ml of remaining 150.0ml @12:41:09.01 Intake.select :open @12:41:11.17 Intake @29.6psia, vacuum from 20.0 to 24.0psi @12:41:11.19 SS.reconfigure forSampling @12:41:11.25 SS.seek 25.00ml @12:41:35.94 Stroke completed -- waiting for pressure to equalize ... @12:41:37.17 Intake.select :close @12:41:37.31 SSV.dial :exhaust @12:41:38.09 Exhaust.select :open @12:41:38.20 SS.reconfigure SSexhale @12:41:38.25 SS.seek 0.00ml @12:41:46.94 Exhaust.select :close @12:41:47.05 SSV.dial CBV @12:41:47.07 CBV.dial SSV @12:41:47.85 Allowing 185s for 25.0ml of remaining 125.0ml @12:41:47.86 Intake.select :open @12:41:50.02 Intake @29.4psia, vacuum from 20.0 to 24.0psi @12:41:50.04 SS.reconfigure forSampling @12:41:50.10 SS.seek 25.00ml @12:42:14.98 Stroke completed -- waiting for pressure to equalize ... @12:42:16.21 Intake.select :close @12:42:16.35 SSV.dial :exhaust @12:42:17.12 Exhaust.select :open @12:42:17.23 SS.reconfigure SSexhale @12:42:17.28 SS.seek 0.00ml @12:42:25.89 Exhaust.select :close @12:42:26.00 SSV.dial CBV @12:42:26.02 CBV.dial SSV @12:42:26.80 Allowing 185s for 25.0ml of remaining 100.0ml @12:42:26.82 Intake.select :open @12:42:28.98 Intake @29.2psia, vacuum from 20.0 to 24.0psi @12:42:28.99 SS.reconfigure forSampling @12:42:29.05 SS.seek 25.00ml @12:42:46.12 delay 3 seconds @12:42:49.13 ATV.dial :air3 @12:42:49.46 delay 3 seconds @12:42:52.52 AS.seek 0.604ml @12:42:53.92 Stroke completed -- waiting for pressure to equalize ... @12:42:55.15 Intake.select :close @12:42:55.29 SSV.dial :exhaust @12:42:56.07 Exhaust.select :open @12:42:56.69 SS.reconfigure SSexhale @12:42:56.74 SS.seek 0.00ml @12:42:57.93 delay 3 seconds @12:43:00.94 ATV.dial :bleach @12:43:01.32 AS.seek 0.589ml @12:43:04.63 delay 3 seconds @12:43:05.87 Exhaust.select :close @12:43:05.97 SSV.dial CBV @12:43:05.99 CBV.dial SSV @12:43:06.77 Allowing 185s for 25.0ml of remaining 75.0ml @12:43:06.78 Intake.select :open @12:43:07.64 ATV.dial :ASV @12:43:08.63 ASV.dial :wasteR @12:43:08.93 Intake @29.0psia, vacuum from 20.0 to 24.0psi @12:43:08.95 SS.reconfigure forSampling @12:43:09.02 SS.seek 25.00ml @12:43:09.28 AS.seek 0.000ml @12:43:33.75 Stroke completed -- waiting for pressure to equalize ... @12:43:35.57 Intake.select :close @12:43:35.72 SSV.dial :exhaust @12:43:36.49 Exhaust.select :open @12:43:36.60 SS.reconfigure SSexhale @12:43:36.65 SS.seek 0.00ml @12:43:45.25 Exhaust.select :close @12:43:45.35 SSV.dial CBV @12:43:45.37 CBV.dial SSV @12:43:46.14 Allowing 185s for 25.0ml of remaining 50.0ml @12:43:46.16 Intake.select :open @12:43:48.30 Intake @28.9psia, vacuum from 20.0 to 24.0psi @12:43:48.32 SS.reconfigure forSampling @12:43:48.38 SS.seek 25.00ml @12:44:13.27 Stroke completed -- waiting for pressure to equalize ... @12:44:15.09 Intake.select :close @12:44:15.23 SSV.dial :exhaust @12:44:16.00 Exhaust.select :open @12:44:16.10 SS.reconfigure SSexhale @12:44:16.15 SS.seek 0.00ml @12:44:25.25 Exhaust.select :close @12:44:25.35 SSV.dial CBV @12:44:25.37 CBV.dial SSV @12:44:26.16 Allowing 185s for 25.0ml of remaining 25.0ml @12:44:26.18 Intake.select :open @12:44:28.34 Intake @28.7psia, vacuum from 20.0 to 24.0psi @12:44:28.36 SS.reconfigure forSampling @12:44:28.42 SS.seek 24.96ml @12:44:53.11 Stroke completed -- waiting for pressure to equalize ... @12:44:54.40 Intake.select :close @12:44:54.55 SSV.dial :exhaust @12:44:55.34 Exhaust.select :open @12:44:55.46 SS.reconfigure SSexhale @12:44:55.51 SS.seek 0.00ml @12:45:04.61 Exhaust.select :close @12:45:04.72 Sampled 1000.0ml @12:45:04.73 collectSample: Complete @12:45:04.75 Drying puck before fixing for 04:00.00 @12:45:04.76 CBV.dial :waste2b @12:45:05.53 delay 1:00 @12:45:09.75 delay 1:00 @12:46:05.53 CSV.dial :air @12:46:05.99 CS.reconfigure CSfast @12:46:06.04 CS.seek 10.000ml @12:46:09.76 AFV.select :carrierSolution @12:46:09.86 delay 2 seconds @12:46:11.87 AS.reconfigure ASconfig @12:46:11.92 AS.seek 1.000ml @12:46:29.20 CBV.dial between CSV and waste2b @12:46:29.54 Pumping wcr puck from tube 2 up to 55psi... @12:46:29.60 CBV.dial SSV @12:46:31.04 CBV.dial between CSV and waste2b @12:46:31.99 CSV.dial between air and CTV @12:46:32.40 CS.seek 6.849ml @12:46:40.31 CTV.dial CSV,avoiding => air @12:46:40.33 CSV.dial CTV @12:46:41.09 CS.reconfigure CSpressurize @12:46:41.14 CS.seek 0.000ml @12:46:57.13 CBV.dial :waste2b @12:46:57.46 CS.reconfigure CSconfig @12:46:57.51 CS.seek empty @12:47:00.01 delay 1:00 @12:47:10.54 delay 1 second @12:47:11.55 AFV.select :holdingCoil @12:47:11.65 delay 1 second @12:47:12.66 ATV.dial :ASV @12:47:12.67 ASV.dial :wasteR @12:47:12.69 AS.reconfigure ASslow1 @12:47:12.74 AS.seek 0.000ml @12:48:00.02 CTV.dial :air,avoiding => intake @12:48:01.32 Fix #1 of 2 (with rnal) for 02:00.00 @12:48:01.33 CS Pulling 2ml of rnal @12:48:01.35 CSV.dial CSR @12:48:01.79 CSR.select :rnal @12:48:01.92 CS.seek 2.000ml @12:48:07.88 delay 5 seconds @12:48:12.89 CSR.select :air @12:48:12.99 CS.reconfigure CSfast @12:48:13.04 CS.seek 10.000ml @12:48:31.70 CBV.dial between CSV and waste2b @12:48:32.57 Pumping wcr puck from tube 2 up to 55psi... @12:48:32.63 CBV.dial SSV @12:48:34.09 CBV.dial between CSV and waste2b @12:48:35.04 CSV.dial between air and CTV @12:48:35.72 CS.seek 6.873ml @12:48:43.55 CTV.dial CSV,avoiding => air @12:48:43.57 CSV.dial CTV @12:48:44.91 CS.reconfigure CSpressurize @12:48:44.96 CS.seek 0.000ml @12:49:02.28 delay 2:00 @12:50:37.09 delay 20 seconds @12:50:57.10 ATV.dial :air3 @12:50:58.21 AS.seek 0.590ml @12:51:02.29 CBV.dial :waste2b @12:51:02.62 CS.reconfigure CSconfig @12:51:02.67 CS.seek empty @12:51:04.13 delay 1:00 @12:52:04.15 CTV.dial :air,avoiding => intake @12:52:05.45 Fix #2 of 2 (with rnal) for 02:00.00 @12:52:05.46 CS Pulling 2ml of rnal @12:52:05.48 CSV.dial CSR @12:52:05.92 CSR.select :rnal @12:52:06.04 CS.seek 2.000ml @12:52:11.94 delay 5 seconds @12:52:16.95 CSR.select :air @12:52:17.05 CS.reconfigure CSfast @12:52:17.10 CS.seek 10.000ml @12:52:35.81 CBV.dial between CSV and waste2b @12:52:36.16 Pumping wcr puck from tube 2 up to 55psi... @12:52:36.22 CBV.dial SSV @12:52:37.69 CBV.dial between CSV and waste2b @12:52:38.64 CSV.dial between air and CTV @12:52:39.30 CS.seek 6.776ml @12:52:47.35 CTV.dial CSV,avoiding => air @12:52:47.37 CSV.dial CTV @12:52:48.68 CS.reconfigure CSpressurize @12:52:48.73 CS.seek 0.000ml @12:52:58.85 delay 3 seconds @12:53:01.86 ATV.dial :ASV @12:53:02.93 ASV.dial :wasteR @12:53:03.00 AS.seek 0.090ml @12:53:05.45 delay 2:00 @12:54:45.21 delay 30 seconds @12:55:05.46 CBV.dial :waste2b @12:55:05.80 CS.reconfigure CSconfig @12:55:05.85 CS.seek empty @12:55:07.46 delay 1:00 @12:55:15.22 ATV.dial :extSrc @12:55:15.74 delay 3 seconds @12:55:18.75 ATV.dial :ElutionWater @12:55:19.68 AS.seek 0.120ml @12:55:26.04 ATV.dial :air3 @12:55:26.52 AS.seek 0.130ml @12:55:28.85 delay 2 seconds @12:55:30.86 ATV.dial :bleach @12:55:31.24 AS.seek 0.230ml @12:55:51.90 delay 3 seconds @12:55:54.91 ATV.dial :air3 @12:55:55.25 delay 3 seconds @12:55:58.31 AS.seek 0.255ml @12:56:03.66 delay 3 seconds @12:56:06.67 ATV.dial :bleach @12:56:07.07 AS.seek 0.240ml @12:56:07.47 CTV.dial :air,avoiding => intake @12:56:08.80 Storing wcr puck in Collection Clamp to tube 1 @12:56:08.96 SC.reconfigure SCtight @12:56:09.01 SC.seek 1 @12:56:10.39 delay 3 seconds @12:56:11.27 SC.reconfigure SCloose @12:56:11.46 Elbow.reconfigure TightElbow @12:56:11.51 Elbow.seek 1 @12:56:13.12 Forearm.reconfigure TightForearm @12:56:13.17 Forearm.seek 1 + 800 counts @12:56:13.40 ATV.dial :ASV @12:56:14.31 ASV.dial :SPEdelivery @12:56:14.36 SE.reconfigure SEpick @12:56:14.44 SE.seek beamBreak @12:56:14.79 AFV.select :carrierSolution @12:56:14.89 delay 2 seconds @12:56:16.90 AS.reconfigure ASconfig @12:56:16.95 AS.seek 0.500ml @12:56:17.42 SE.configure SEconfig @12:56:17.56 Elbow.reconfigure LimpElbow @12:56:17.61 Forearm.reconfigure LooseForearm @12:56:17.66 Forearm.seek 1 @12:56:18.46 Elbow.hold @12:56:18.52 Elbow.reconfigure TightElbow @12:56:18.61 SE.seek 1.05 Pucks below Top Plate @12:56:18.98 Forearm.coast @12:56:19.03 Forearm.seek 1 + 347 counts @12:56:19.34 Forearm.reconfigure TightForearm @12:56:19.39 Forearm.seek 1 + 800 counts @12:56:19.57 SE.seek 2.05 Pucks below Top Plate @12:56:20.14 Forearm.reconfigure LooseForearm @12:56:20.19 Forearm.seek retract @12:56:21.15 Elbow.seek CC @12:56:22.75 Elbow.reconfigure LooseElbow @12:56:22.85 Forearm.seek CC @12:56:23.81 CC.seek sealed @12:56:24.77 Hand.grip @12:56:25.51 CC.seek open @12:56:28.91 Forearm.seek retract @12:56:29.87 Elbow.reconfigure TightElbow @12:56:29.92 Elbow.seek 1 @12:56:31.50 Elbow.reconfigure LimpElbow @12:56:31.56 Forearm.seek 1 @12:56:32.39 delay 1 second @12:56:32.58 Elbow.hold @12:56:32.66 Elbow.reconfigure TightElbow @12:56:32.71 Hand.release @12:56:33.40 AFV.select :holdingCoil @12:56:33.63 AS.reconfigure ASslow1 @12:56:33.69 AS.seek 0.170ml @12:56:33.71 Forearm.coast @12:56:33.79 Forearm.seek 1 + 324 counts @12:56:34.04 Forearm.reconfigure TightForearm @12:56:34.09 Forearm.seek 1 + 800 counts @12:56:34.52 SE.seek down @12:56:35.63 Forearm.reconfigure LooseForearm @12:56:35.68 Forearm.seek retract @12:56:37.04 SE.coast @12:56:37.08 SC.coast @12:56:37.13 Starting finalPurge @12:56:37.14 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:56:37.31 Elbow.seek FlushPuck::Garage @12:56:39.11 Elbow.reconfigure LooseElbow @12:56:39.21 Forearm.seek garage @12:56:40.27 Hand.grip @12:56:41.10 Forearm.seek retract @12:56:42.15 Elbow.reconfigure TightElbow @12:56:42.19 Elbow.seek CC @12:56:45.00 Elbow.reconfigure LooseElbow @12:56:45.09 Forearm.reconfigure TightForearm @12:56:45.13 Forearm.seek CC @12:56:46.30 CC.seek closed @12:56:50.11 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.94 @12:56:50.18 Hand.release @12:56:51.48 Forearm.reconfigure LooseForearm @12:56:51.53 Forearm.seek retract @12:56:52.47 Flushing the Collection Valve::Manifold @12:56:52.70 CS Pulling 5ml of flush @12:56:52.71 CSV.dial CSR @12:56:53.16 CSR.select :flush @12:56:53.27 CS.seek 5.000ml @12:57:06.80 delay 5 seconds @12:57:11.81 CSR.select :air @12:57:11.97 CS.reconfigure CSfast @12:57:12.01 CS.seek 7.500ml @12:57:18.39 CSV.dial :waste @12:57:19.67 CS.reconfigure CSconfig @12:57:19.71 CS.seek 0.000ml @12:57:39.51 Flushing lower manifold's waste1 port @12:57:39.63 CSV.dial :flush @12:57:40.10 CS.reconfigure CSfast @12:57:40.14 CS.seek 5.000ml @12:57:41.30 SPE.hold 2:46:40,95.0C @12:57:41.32 delay 3:00 @12:57:52.64
delay 5 seconds @12:57:57.65 CSV.dial :air @12:57:58.16 CS.seek 7.000ml @12:58:03.73 CSV.dial CTV @12:58:03.77
CTV.dial CSV @12:58:05.15 CBV.dial :waste1 @12:58:05.96 CS.seek 0.000ml @12:58:23.29 Flushing lower manifold's waste2b port @12:58:23.40 CSV.dial :flush @12:58:24.26 CS.seek 5.000ml @12:58:36.89 delay 5 seconds @12:58:41.90 CSV.dial :air @12:58:42.70 CS.seek 7.000ml @12:58:48.26 CSV.dial CTV @12:58:48.30
CTV.dial CSV @12:58:48.85 CBV.dial :waste2b @12:58:49.65 CS.seek 0.000ml @12:59:06.92 Flushing upper manifold @12:59:07.04 CSV.dial :flush @12:59:07.90 CS.seek 5.000ml @12:59:20.63 delay 5 seconds @12:59:25.64 CSV.dial :air @12:59:26.23 CS.seek 7.000ml @12:59:31.85 CSV.dial CBV @12:59:31.89
CBV.dial CSV @12:59:33.07 CTV.dial :air @12:59:34.44 CS.seek 0.000ml @12:59:51.91 delay 7 seconds @12:59:58.92 CS.seek 3.000ml @13:00:06.85 CSV.dial :waste @13:00:07.39 CS.seek 0.000ml @13:00:15.44 CSV.dial :flush @13:00:15.89 CS.seek 3.000ml @13:00:23.92 delay 5 seconds @13:00:28.93 CSV.dial :air @13:00:29.51 CS.seek 4.999ml @13:00:35.05 CSV.dial :air @13:00:35.07 CS.seek 0.000ml @13:00:41.34 AFV.select :carrierSolution @13:00:41.44 delay 2 seconds @13:00:43.45 AS.reconfigure ASconfig @13:00:43.50 AS.seek 1.000ml @13:00:47.67
delay 7 seconds @13:00:54.68 CS.seek 3.000ml @13:01:02.48 CSV.dial :waste @13:01:03.31 CS.seek 0.000ml @13:01:11.14 Disinfecting external Intake with mfbkill @13:01:11.26 CSV.dial CSR @13:01:12.65 CSR.select :mfbkill @13:01:12.77 CS.seek 8.000ml @13:01:32.19 delay 1 second @13:01:32.23
delay 5 seconds @13:01:33.20 AFV.select :holdingCoil @13:01:33.30 delay 1 second @13:01:34.31 ASV.dial :wasteD @13:01:35.33 AS.seek 0.000ml @13:01:37.24
CSR.select :air @13:01:37.35 CS.seek 10.000ml @13:01:43.01 CSV.dial CBV @13:01:43.03
CBV.dial CSV @13:01:44.05 CTV.dial :intake @13:01:44.79 Intake.select :open @13:01:44.90 CS.seek 2.000ml @13:02:04.64 Intake.select :close @13:02:04.75 CTV.dial :air @13:02:05.54 CS.reconfigure CSconfig @13:02:05.58 CS.seek 0.000ml @13:02:11.54 Flushing lower manifold's waste1 port @13:02:11.56 CS Pulling 4ml of mfbkill @13:02:11.57 CSV.dial CSR @13:02:12.68 CSR.select :mfbkill @13:02:12.79 CS.seek 4.000ml @13:02:24.39 delay 5 seconds @13:02:29.40 CSR.select :air @13:02:29.53 CS.reconfigure CSfast @13:02:29.58 CS.seek 10.000ml @13:02:33.92 Priming Eluate @13:02:33.93 AFV.select :carrierSolution @13:02:34.04 delay 2 seconds @13:02:36.05 AS.seek 0.500ml @13:02:44.15 CSV.dial CTV @13:02:44.17
CTV.dial CSV @13:02:45.52 CBV.dial :waste1 @13:02:46.00 CS.seek 0.000ml @13:03:05.48 delay 1 second @13:03:06.49 AFV.select :holdingCoil @13:03:06.59 ATV.dial ASV @13:03:06.60 ASV.dial :SPErecovery @13:03:07.27 Swish #1 of 2 @13:03:07.34 AS.reconfigure ASslow0 @13:03:07.39 AS.seek 0.600ml @13:03:09.58
Flushing lower manifold's waste2b port CS Pulling 4ml of mfbkill @13:03:09.60 CSV.dial CSR @13:03:10.15 CSR.select :mfbkill @13:03:10.26 CS.reconfigure CSconfig @13:03:10.31 CS.seek 4.000ml @13:03:17.94 AS.seek 0.500ml @13:03:21.32
delay 5 seconds @13:03:26.33 CSR.select :air @13:03:26.44 CS.reconfigure CSfast @13:03:26.49 CS.seek 10.000ml @13:03:28.44 Swish #2 of 2 @13:03:28.52 AS.seek 0.600ml @13:03:39.03 AS.seek 0.500ml @13:03:40.95 CSV.dial CTV @13:03:40.97
CTV.dial CSV @13:03:41.52 CBV.dial :waste2b @13:03:42.30 CS.seek 0.000ml @13:03:49.50 delay 3 seconds @13:03:52.56 AS.reconfigure ASslow1 @13:03:52.61 AS.seek 0.676ml @13:04:05.60
Killing upper manifold @13:04:05.61 CS Pulling 4ml of mfbkill @13:04:05.62 CSV.dial CSR @13:04:06.18 CSR.select :mfbkill @13:04:06.29 CS.reconfigure CSconfig @13:04:06.34 CS.seek 4.000ml @13:04:17.35 delay 5 seconds @13:04:22.36 CSR.select :air @13:04:22.46 CS.reconfigure CSfast @13:04:22.51 CS.seek 10.000ml @13:04:28.76 delay 3 seconds @13:04:31.77 Eluate primed @13:04:31.78 ATV.dial :waste2 @13:04:32.32 AS.reconfigure ASnorm @13:04:32.36 AS.seek 0.000ml @13:04:36.88 CSV.dial CBV @13:04:36.92
CBV.dial CSV @13:04:37.96 CTV.dial :air @13:04:39.30 CS.seek 0.000ml @13:05:02.60 delay 7 seconds @13:05:07.21 SPE.coast @13:05:07.30 ASV.dial :SPErecovery @13:05:07.32 Setting up PCR for HF183em -- writing /var/log/esp/HF183em14jan2213h250ml.pcr @13:05:07.33 Building PCR Slug from ASV with MM2 mix, PP4 primer @13:05:07.35 Priming with carrier @13:05:07.37 ATV.dial ARV @13:05:08.07 ARV.dial :carrier @13:05:08.37 AS.seek 0.500ml @13:05:09.61
CS.seek 3.000ml @13:05:17.13 CSV.dial :waste @13:05:17.58 CS.seek 0.000ml @13:05:25.16 Flushing upper manifold @13:05:25.28 CSV.dial :flush @13:05:25.93 CS.seek 5.000ml @13:05:34.31 AFV.select :carrierSolution @13:05:34.41 delay 2 seconds @13:05:36.42 AS.reconfigure ASconfig @13:05:36.47 AS.seek 1.000ml @13:05:37.91
delay 5 seconds @13:05:42.92 CSV.dial :air @13:05:43.50 CS.seek 7.000ml @13:05:48.78 CSV.dial CBV @13:05:48.80
CBV.dial CSV @13:05:49.89 CTV.dial :air @13:05:49.91 CS.seek 0.000ml @13:06:05.92 delay 1 second @13:06:06.25
delay 7 seconds @13:06:06.94 AFV.select :holdingCoil @13:06:07.04 delay 1 second @13:06:08.05 ATV.dial :waste @13:06:09.32 AS.reconfigure ASnorm @13:06:09.37 AS.seek empty @13:06:13.26
CS.seek 3.000ml @13:06:20.78 CSV.dial :waste @13:06:21.31 CS.seek 0.000ml @13:06:28.89 CSV.dial :flush @13:06:29.34 CS.seek 3.000ml @13:06:36.91 delay 5 seconds @13:06:41.92 CSV.dial :air @13:06:42.41 CS.seek 5.000ml @13:06:47.75 CSV.dial :air @13:06:47.77 CS.seek 0.000ml @13:06:59.69 delay 7 seconds @13:07:00.85 AFV.select :carrierSolution @13:07:00.95 delay 2 seconds @13:07:02.96 AS.reconfigure ASconfig @13:07:03.01 AS.seek 0.500ml @13:07:07.22
CS.seek 3.000ml @13:07:14.72 CSV.dial :waste @13:07:15.52 CS.seek 0.000ml @13:07:22.97 Flushing lower manifold's waste1 port @13:07:23.09 CSV.dial :flush @13:07:23.62 CS.seek 5.000ml @13:07:32.51 delay 1 second @13:07:33.52 AFV.select :holdingCoil @13:07:33.62 Building Slug @13:07:33.63 ATV.dial :ARV @13:07:34.90 delay 2 seconds @13:07:35.50
delay 5 seconds @13:07:36.91 ARV.dial :air1 @13:07:37.27 delay 2 seconds @13:07:39.32 AS.reconfigure ASslow2 @13:07:39.37 AS.seek 0.506ml @13:07:40.51
CSV.dial :air @13:07:41.01 CS.seek 7.000ml @13:07:45.80 delay 2 seconds @13:07:46.40 CSV.dial CTV @13:07:46.43
CTV.dial CSV @13:07:47.78 CBV.dial :waste1 @13:07:47.83 ARV.dial :MM2 @13:07:48.26
CS.seek 0.000ml @13:07:49.08 delay 2 seconds @13:07:51.08 Adding MM2 @13:07:51.14 AS.seek 0.524ml @13:08:04.78
Flushing lower manifold's waste2b port @13:08:04.89 CSV.dial :flush @13:08:05.69 CS.seek 5.000ml @13:08:09.85 delay 25 seconds @13:08:17.59
delay 5 seconds @13:08:22.60 CSV.dial :air @13:08:23.17 CS.seek 6.999ml @13:08:28.59 CSV.dial CTV @13:08:28.63
CTV.dial CSV @13:08:29.06 CBV.dial :waste2b @13:08:29.83 CS.seek 0.000ml @13:08:34.86 ARV.dial :PP4 @13:08:35.22 Adding PP4 @13:08:35.23 delay 2 seconds @13:08:37.29 AS.seek 0.530ml @13:08:43.75 delay 10 seconds @13:08:46.12
Disinfecting external Intake with kill @13:08:46.23 CSV.dial CSR @13:08:46.66 CSR.select :kill @13:08:46.77 CS.seek 8.000ml @13:08:53.76 ARV.dial :air3 @13:08:54.20 adding ARV air @13:08:54.21 delay 2 seconds @13:08:56.27 AS.seek 0.536ml @13:09:02.75 delay 5 seconds @13:09:05.30
delay 5 seconds @13:09:07.76 ARV.dial :carrier @13:09:08.83 delay 7 seconds @13:09:10.31
CSR.select :air @13:09:10.41 CS.seek 10.000ml @13:09:15.71 CSV.dial CBV @13:09:15.73
CBV.dial CSV @13:09:15.88 AS.seek 0.594ml @13:09:16.77
CTV.dial :intake @13:09:17.51 Intake.select :open @13:09:17.61 CS.seek 2.000ml @13:09:36.71 Intake.select :close @13:09:36.81 CTV.dial :air @13:09:37.55 CS.reconfigure CSconfig @13:09:37.59 CS.seek 0.000ml @13:09:43.55 CSV.dial CSR @13:09:44.62 CSR.select :flush @13:09:44.73 CS.seek 5.000ml @13:09:58.33 delay 5 seconds @13:10:03.35 CSR.select :air @13:10:03.46 CS.seek 10.000ml @13:10:15.41 delay 5 seconds @13:10:16.96
CSV.dial CBV @13:10:18.03 CS.seek 0.000ml @13:10:20.42 ATV.dial :ASV @13:10:20.76 delay 2 seconds @13:10:22.81 AS.seek 0.600ml @13:10:29.11 delay 4 seconds @13:10:33.12 ATV.dial ARV @13:10:33.47 delay 2 seconds @13:10:35.48 ARV.dial :carrier @13:10:35.49 delay 2 seconds @13:10:37.56 AS.seek 0.650ml @13:10:44.21
Garaging FlushPuck in Collection Clamp @13:10:44.29 Elbow.reconfigure TightElbow @13:10:44.34 Elbow.seek CC @13:10:44.45 Elbow.reconfigure LooseElbow @13:10:44.55 Forearm.seek CC @13:10:45.53 CC.seek sealed @13:10:46.99 Hand.grip @13:10:47.73 CC.seek open @13:10:51.05 Forearm.seek retract @13:10:52.04 Elbow.reconfigure TightElbow @13:10:52.08 Elbow.seek garage @13:10:55.02 Elbow.reconfigure LooseElbow @13:10:55.11 Forearm.reconfigure TightForearm @13:10:55.16 Forearm.seek garage @13:10:56.35 Hand.release @13:10:57.15 Forearm.reconfigure LooseForearm @13:10:57.20 Forearm.seek retract @13:11:28.73 delay 4 seconds @13:11:32.74 ATV.dial PCR @13:11:33.25 ASV.dial :waste1 @13:11:33.94 ARV.dial :air1 @13:11:34.29 Positioning Slug by 0.324ml @13:11:34.36 AS.seek 0.326ml @13:17:04.86 delay 5 seconds @13:17:09.87 ATV.dial ARV @13:17:10.41 ASV.dial :PCRlock @13:17:10.75 delay 5 seconds @13:17:15.76 Running HF183em ProfileEM60C2 thermocycling @13:17:15.77 PCR.program ProfileEM60C2 @13:17:15.96 ProfileEM60C2 should complete in < 2:11:38 @13:17:15.97 PCR.run 2:49:32.5 @15:14:27.44 PCR.off @15:14:27.97 PCR for HF183em completed -- closed /var/log/esp/HF183em14jan2213h250ml.pcr @15:14:28.03 Cleaning PCR @15:14:28.04 AFV.select :carrierSolution @15:14:28.15 delay 2 seconds @15:14:30.16 AS.reconfigure ASconfig @15:14:30.21 AS.seek 0.500ml @15:14:40.66 delay 1 second @15:14:41.67 AFV.select :holdingCoil @15:14:41.77 ASV.dial :waste1 @15:14:42.25 ATV.dial :air3 @15:14:43.73 AS.reconfigure ASslow1 @15:14:43.78 AS.seek 0.510ml @15:14:46.10 delay 2 seconds @15:14:48.11 ATV.dial :bleach @15:14:48.60 AS.seek 0.810ml @15:15:50.03 delay 3 seconds @15:15:53.04 ATV.dial :air3 @15:15:53.38 delay 3 seconds @15:15:56.44 AS.seek 0.835ml @15:16:01.78 delay 3 seconds @15:16:04.79 ATV.dial :bleach @15:16:05.19 AS.seek 0.820ml @15:16:08.50 delay 3 seconds @15:16:11.51 ARV.dial :waste @15:16:12.02 delay 3 seconds @15:16:15.03 ATV.dial :ARV @15:16:15.77 AS.reconfigure ASconfig @15:16:15.82 AS.seek 0.470ml @15:16:36.53 AFV.select :carrierSolution @15:16:36.64 delay 2 seconds @15:16:38.65 AS.seek 1.000ml @15:17:09.87 delay 1 second @15:17:10.88 AFV.select :holdingCoil @15:17:10.98 delay 1 second @15:17:11.99 AS.reconfigure ASfast @15:17:12.04 AS.seek 0.000ml @15:17:39.76 AFV.select :carrierSolution @15:17:39.87 delay 2 seconds @15:17:41.88 AS.reconfigure ASconfig @15:17:41.93 AS.seek 1.000ml @15:18:40.55 delay 1 second @15:18:41.56 AFV.select :holdingCoil @15:18:41.67 delay 1 second @15:18:42.68 AS.reconfigure ASfast @15:18:42.73 AS.seek 0.000ml @15:19:10.49 AFV.select :carrierSolution @15:19:10.60 delay 2 seconds @15:19:12.61 AS.reconfigure ASconfig @15:19:12.66 AS.seek 1.000ml @15:20:11.28 delay 1 second @15:20:12.29 AFV.select :holdingCoil @15:20:12.39 delay 1 second @15:20:13.40 AS.reconfigure ASfast @15:20:13.45 AS.seek 0.000ml @15:20:41.19 ARV.dial between carrier and air1 @15:20:41.61 ATV.dial :air3 @15:20:42.57 AS.reconfigure ASslow1 @15:20:42.62 AS.seek 0.010ml @15:20:44.92 delay 2 seconds @15:20:46.93 ATV.dial :bleach @15:20:47.33 AS.seek 0.810ml @15:23:30.73 delay 3 seconds @15:23:33.74 ATV.dial :air3 @15:23:34.09 delay 3 seconds @15:23:37.15 AS.seek 0.835ml @15:23:42.51 delay 3 seconds @15:23:45.52 ATV.dial :bleach @15:23:46.01 AS.seek 0.820ml @15:23:49.31 delay 3 seconds @15:23:52.32 ATV.dial PCR @15:23:52.66 AS.reconfigure ASconfig @15:23:52.70 AS.seek empty @15:24:40.82 PCR.program :bleachProfile @15:24:40.89 PCR program should complete in < 2:45 @15:24:40.90 PCR.run 10:00 @15:27:39.11 PCR.off @15:27:39.14 AFV.select :carrierSolution @15:27:39.24 delay 2 seconds @15:27:41.25 AS.seek 1.000ml @15:28:39.91 delay 1 second @15:28:40.92 AFV.select :holdingCoil @15:28:41.03 delay 1 second @15:28:42.04 AS.reconfigure ASfast @15:28:42.08 AS.seek 0.000ml @15:29:09.84 delay 3 seconds @15:29:12.85 AFV.select :carrierSolution @15:29:12.95 delay 2 seconds @15:29:14.96 AS.reconfigure ASconfig @15:29:15.01 AS.seek 1.000ml @15:30:13.64 delay 1 second @15:30:14.65 AFV.select :holdingCoil @15:30:14.75 delay 1 second @15:30:15.76 AS.reconfigure ASfast @15:30:15.81 AS.seek 0.000ml @15:30:43.53 delay 3 seconds @15:30:46.54 AFV.select :carrierSolution @15:30:46.64 delay 2 seconds @15:30:48.65 AS.reconfigure ASconfig @15:30:48.70 AS.seek 1.000ml @15:31:47.31 delay 1 second @15:31:48.32 AFV.select :holdingCoil @15:31:48.42 delay 1 second @15:31:49.43 AS.reconfigure ASfast @15:31:49.48 AS.seek 0.000ml @15:32:17.21 delay 3 seconds @15:32:20.22 AFV.select :carrierSolution @15:32:20.32 delay 2 seconds @15:32:22.33 AS.reconfigure ASconfig @15:32:22.38 AS.seek 1.000ml @15:33:20.99 delay 1 second @15:33:22.00 AFV.select :holdingCoil @15:33:22.10 delay 1 second @15:33:23.11 AS.reconfigure ASfast @15:33:23.16 AS.seek 0.000ml @15:33:50.94 delay 3 seconds @15:33:53.95 AFV.select :carrierSolution @15:33:54.06 delay 2 seconds @15:33:56.07 AS.reconfigure ASconfig @15:33:56.11 AS.seek 1.000ml @15:34:54.72 delay 1 second @15:34:55.73 AFV.select :holdingCoil @15:34:55.83 delay 1 second @15:34:56.84 AS.reconfigure ASnorm @15:34:56.89 AS.seek 0.000ml @15:35:48.37 Setting up PCR for ENTap -- writing /var/log/esp/ENTap14jan2215h250ml.pcr @15:35:48.38 Building PCR Slug from ASV with MM1 mix, PP1 primer @15:35:48.39 Priming with carrier @15:35:48.41 ATV.dial ARV @15:35:49.03 ARV.dial :carrier @15:35:49.33 AS.seek 0.500ml @15:36:15.26 AFV.select :carrierSolution @15:36:15.37 delay 2 seconds @15:36:17.38 AS.reconfigure ASconfig @15:36:17.43 AS.seek 1.000ml @15:36:46.90 delay 1 second @15:36:47.91 AFV.select :holdingCoil @15:36:48.01 delay 1 second @15:36:49.52 ATV.dial :waste @15:36:50.88 AS.reconfigure ASnorm @15:36:50.93 AS.seek empty @15:37:42.41 AFV.select :carrierSolution @15:37:42.51 delay 2 seconds @15:37:44.52 AS.reconfigure ASconfig @15:37:44.58 AS.seek 0.500ml @15:38:14.13 delay 1 second @15:38:15.14 AFV.select :holdingCoil @15:38:15.24 Building Slug @15:38:15.25 ATV.dial :ARV @15:38:16.51 delay 2 seconds @15:38:18.52 ARV.dial :air1 @15:38:18.87 delay 2 seconds @15:38:20.93 AS.reconfigure ASslow2 @15:38:20.98 AS.seek 0.506ml @15:38:27.30 delay 2 seconds @15:38:29.31 ARV.dial :MM1 @15:38:29.82 delay 2 seconds @15:38:31.83 Adding MM1 @15:38:31.90 AS.seek 0.524ml @15:38:50.49 delay 25 seconds @15:39:15.50 ARV.dial :PP1 @15:39:15.86 Adding PP1 @15:39:15.87 delay 2 seconds @15:39:17.93 AS.seek 0.530ml @15:39:24.41 delay 10 seconds @15:39:34.42 ARV.dial :air1 @15:39:34.76 adding ARV air @15:39:34.77 delay 2 seconds @15:39:36.84 AS.seek 0.536ml @15:39:43.32 delay 5 seconds @15:39:48.33 ARV.dial :carrier @15:39:48.66 delay 7 seconds @15:39:55.72 AS.seek 0.594ml @15:40:55.11 delay 5 seconds @15:41:00.12 ATV.dial :ASV @15:41:00.47 delay 2 seconds @15:41:02.53 AS.seek 0.600ml @15:41:08.84 delay 4 seconds @15:41:12.85 ATV.dial ARV @15:41:13.19 delay 2 seconds @15:41:15.20 ARV.dial :carrier @15:41:15.21 delay 2 seconds @15:41:17.27 AS.seek 0.650ml @15:42:08.36 delay 4 seconds @15:42:12.37 ATV.dial PCR @15:42:12.89 ASV.dial :waste1 @15:42:12.90 ARV.dial :air1 @15:42:13.26 Positioning Slug by 0.324ml @15:42:13.33 AS.seek 0.326ml @15:47:44.02 delay 5 seconds @15:47:49.03 ATV.dial ARV @15:47:49.57 ASV.dial :PCRlock @15:47:50.08 delay 5 seconds @15:47:55.09 Running ENTap Profile60C thermocycling @15:47:55.10 PCR.program Profile60C @15:47:55.22 Profile60C should complete in < 1:39:08 @15:47:55.23 PCR.run 2:08:55 @17:12:05.50 PCR.off @17:12:06.04 PCR for ENTap completed -- closed /var/log/esp/ENTap14jan2215h250ml.pcr @17:12:06.10 Cleaning PCR @17:12:06.11 AFV.select :carrierSolution @17:12:06.21 delay 2 seconds @17:12:08.22 AS.reconfigure ASconfig @17:12:08.27 AS.seek 0.500ml @17:12:18.71 delay 1 second @17:12:19.72 AFV.select :holdingCoil @17:12:19.82 ASV.dial :waste1 @17:12:20.58 ATV.dial :air3 @17:12:21.54 AS.reconfigure ASslow1 @17:12:21.59 AS.seek 0.510ml @17:12:23.91 delay 2 seconds @17:12:25.92 ATV.dial :bleach @17:12:26.31 AS.seek 0.810ml @17:13:27.74 delay 3 seconds @17:13:30.75 ATV.dial :air3 @17:13:31.08 delay 3 seconds @17:13:34.14 AS.seek 0.835ml @17:13:39.49 delay 3 seconds @17:13:42.50 ATV.dial :bleach @17:13:42.88 AS.seek 0.820ml @17:13:46.21 delay 3 seconds @17:13:49.22 ARV.dial :waste @17:13:49.82 delay 3 seconds @17:13:52.83 ATV.dial :ARV @17:13:53.57 AS.reconfigure ASconfig @17:13:53.62 AS.seek 0.470ml @17:14:14.33 AFV.select :carrierSolution @17:14:14.44 delay 2 seconds @17:14:16.45 AS.seek 1.000ml @17:14:47.70 delay 1 second @17:14:48.71 AFV.select :holdingCoil @17:14:48.82 delay 1 second @17:14:49.83 AS.reconfigure ASfast @17:14:49.88 AS.seek 0.000ml @17:15:17.61 AFV.select :carrierSolution @17:15:17.72 delay 2 seconds @17:15:19.73 AS.reconfigure ASconfig @17:15:19.78 AS.seek 1.000ml @17:16:18.40 delay 1 second @17:16:19.41 AFV.select :holdingCoil @17:16:19.51 delay 1 second @17:16:20.52 AS.reconfigure ASfast @17:16:20.57 AS.seek 0.000ml @17:16:48.29 AFV.select :carrierSolution @17:16:48.40 delay 2 seconds @17:16:50.41 AS.reconfigure ASconfig @17:16:50.46 AS.seek 1.000ml @17:17:49.07 delay 1 second @17:17:50.08 AFV.select :holdingCoil @17:17:50.19 delay 1 second @17:17:51.20 AS.reconfigure ASfast @17:17:51.25 AS.seek 0.000ml @17:18:18.97 ARV.dial between carrier and air1 @17:18:19.39 ATV.dial :air3 @17:18:20.33 AS.reconfigure ASslow1 @17:18:20.38 AS.seek 0.010ml @17:18:22.78 delay 2 seconds @17:18:24.79 ATV.dial :bleach @17:18:25.18 AS.seek 0.810ml @17:21:08.64 delay 3 seconds @17:21:11.65 ATV.dial :air3 @17:21:12.08 delay 3 seconds @17:21:15.14 AS.seek 0.835ml @17:21:20.50 delay 3 seconds @17:21:23.51 ATV.dial :bleach @17:21:24.00 AS.seek 0.820ml @17:21:27.31 delay 3 seconds @17:21:30.32 ATV.dial PCR @17:21:30.66 AS.reconfigure ASconfig @17:21:30.70 AS.seek empty @17:22:18.84 PCR.program :bleachProfile @17:22:18.90 PCR program should complete in < 2:45 @17:22:18.91 PCR.run 10:00 @17:25:17.11 PCR.off @17:25:17.15 AFV.select :carrierSolution @17:25:17.25 delay 2 seconds @17:25:19.26 AS.seek 1.000ml @17:26:17.94 delay 1 second @17:26:18.95 AFV.select :holdingCoil @17:26:19.06 delay 1 second @17:26:20.07 AS.reconfigure ASfast @17:26:20.12 AS.seek 0.000ml @17:26:47.88 delay 3 seconds @17:26:50.89 AFV.select :carrierSolution @17:26:50.99 delay 2 seconds @17:26:53.00 AS.reconfigure ASconfig @17:26:53.05 AS.seek 1.000ml @17:27:51.64 delay 1 second @17:27:52.65 AFV.select :holdingCoil @17:27:52.76 delay 1 second @17:27:53.77 AS.reconfigure ASfast @17:27:53.82 AS.seek 0.000ml @17:28:21.56 delay 3 seconds @17:28:24.57 AFV.select :carrierSolution @17:28:24.67 delay 2 seconds @17:28:26.68 AS.reconfigure ASconfig @17:28:26.73 AS.seek 1.000ml @17:29:25.32 delay 1 second @17:29:26.33 AFV.select :holdingCoil @17:29:26.43 delay 1 second @17:29:27.44 AS.reconfigure ASfast @17:29:27.49 AS.seek 0.000ml @17:29:55.21 delay 3 seconds @17:29:58.22 AFV.select :carrierSolution @17:29:58.32 delay 2 seconds @17:30:00.33 AS.reconfigure ASconfig @17:30:00.41 AS.seek 1.000ml @17:30:59.58 delay 1 second @17:31:00.59 AFV.select :holdingCoil @17:31:00.70 delay 1 second @17:31:01.71 AS.reconfigure ASfast @17:31:01.76 AS.seek 0.000ml @17:31:29.48 delay 3 seconds @17:31:32.49 AFV.select :carrierSolution @17:31:32.59 delay 2 seconds @17:31:34.60 AS.reconfigure ASconfig @17:31:34.65 AS.seek 1.000ml @17:32:33.26 delay 1 second @17:32:34.27 AFV.select :holdingCoil @17:32:34.37 delay 1 second @17:32:35.38 AS.reconfigure ASnorm @17:32:35.43 AS.seek 0.000ml @17:33:26.90 Setting up PCR for ENTem -- writing /var/log/esp/ENTem14jan2217h250ml.pcr @17:33:26.91 Building PCR Slug from ASV with MM2 mix, PP3 primer @17:33:26.92 Priming with carrier @17:33:26.94 ATV.dial ARV @17:33:27.45 ARV.dial :carrier @17:33:27.75 AS.seek 0.500ml @17:33:53.67 AFV.select :carrierSolution @17:33:53.77 delay 2 seconds @17:33:55.78 AS.reconfigure ASconfig @17:33:55.83 AS.seek 1.000ml @17:34:25.28 delay 1 second @17:34:26.29 AFV.select :holdingCoil @17:34:26.41 delay 1 second @17:34:27.42 ATV.dial :waste @17:34:28.68 AS.reconfigure ASnorm @17:34:28.73 AS.seek empty @17:35:20.23 AFV.select :carrierSolution @17:35:20.34 delay 2 seconds @17:35:22.35 AS.reconfigure ASconfig @17:35:22.40 AS.seek 0.500ml @17:35:51.88 delay 1 second @17:35:52.89 AFV.select :holdingCoil @17:35:52.99 Building Slug @17:35:53.00 ATV.dial :ARV @17:35:54.35 delay 2 seconds @17:35:56.36 ARV.dial :air1 @17:35:56.70 delay 2 seconds @17:35:58.76 AS.reconfigure ASslow2 @17:35:58.81 AS.seek 0.506ml @17:36:05.16 delay 2 seconds @17:36:07.17 ARV.dial :MM2 @17:36:08.40 delay 2 seconds @17:36:10.41 Adding MM2 @17:36:10.47 AS.seek 0.524ml @17:36:28.98 delay 25 seconds @17:36:53.99 ARV.dial :PP3 @17:36:54.34 Adding PP3 @17:36:54.35 delay 2 seconds @17:36:56.41 AS.seek 0.530ml @17:37:02.72 delay 10 seconds @17:37:12.73 ARV.dial :air2 @17:37:13.06 adding ARV air @17:37:13.07 delay 2 seconds @17:37:15.13 AS.seek 0.536ml @17:37:21.40 delay 5 seconds @17:37:26.41 ARV.dial :carrier @17:37:27.46 delay 7 seconds @17:37:34.52 AS.seek 0.594ml @17:38:33.99 delay 5 seconds @17:38:39.00 ATV.dial :ASV @17:38:39.35 delay 2 seconds @17:38:41.41 AS.seek 0.600ml @17:38:47.96 delay 4 seconds @17:38:51.97 ATV.dial ARV @17:38:52.32 delay 2 seconds @17:38:54.33 ARV.dial :carrier @17:38:54.34 delay 2 seconds @17:38:56.40 AS.seek 0.650ml @17:39:47.77 delay 4 seconds @17:39:51.78 ATV.dial PCR @17:39:52.30 ASV.dial :waste1 @17:39:52.31 ARV.dial :air1 @17:39:52.68 Positioning Slug by 0.324ml @17:39:52.75 AS.seek 0.326ml @17:45:23.43 delay 5 seconds @17:45:28.44 ATV.dial ARV @17:45:28.97 ASV.dial :PCRlock @17:45:29.43 delay 5 seconds @17:45:34.44 Running ENTem ProfileEM60C thermocycling @17:45:34.45 PCR.program ProfileEM60C @17:45:34.58 ProfileEM60C should complete in < 2:11:38 @17:45:34.59 PCR.run 2:49:32.5 @19:42:46.03 PCR.off @19:42:46.57 PCR for ENTem completed -- closed /var/log/esp/ENTem14jan2217h250ml.pcr @19:42:46.63 Cleaning PCR @19:42:46.64 AFV.select :carrierSolution @19:42:46.74 delay 2 seconds @19:42:48.75 AS.reconfigure ASconfig @19:42:48.80 AS.seek 0.500ml @19:42:59.26 delay 1 second @19:43:00.27 AFV.select :holdingCoil @19:43:00.37 ASV.dial :waste1 @19:43:00.83 ATV.dial :air3 @19:43:01.77 AS.reconfigure ASslow1 @19:43:01.82 AS.seek 0.510ml @19:43:04.13 delay 2 seconds @19:43:06.14 ATV.dial :bleach @19:43:06.52 AS.seek 0.810ml @19:44:07.96 delay 3 seconds @19:44:10.97 ATV.dial :air3 @19:44:11.30 delay 3 seconds @19:44:14.36 AS.seek 0.835ml @19:44:19.71 delay 3 seconds @19:44:22.72 ATV.dial :bleach @19:44:23.21 AS.seek 0.820ml @19:44:26.52 delay 3 seconds @19:44:29.53 ARV.dial :waste @19:44:30.03 delay 3 seconds @19:44:33.04 ATV.dial :ARV @19:44:33.87 AS.reconfigure ASconfig @19:44:33.92 AS.seek 0.470ml @19:44:54.63 AFV.select :carrierSolution @19:44:54.74 delay 2 seconds @19:44:56.75 AS.seek 1.000ml @19:45:27.97 delay 1 second @19:45:28.98 AFV.select :holdingCoil @19:45:29.09 delay 1 second @19:45:30.10 AS.reconfigure ASfast @19:45:30.15 AS.seek 0.000ml @19:45:57.87 AFV.select :carrierSolution @19:45:57.98 delay 2 seconds @19:45:59.99 AS.reconfigure ASconfig @19:46:00.06 AS.seek 1.000ml @19:46:58.67 delay 1 second @19:46:59.68 AFV.select :holdingCoil @19:46:59.78 delay 1 second @19:47:00.79 AS.reconfigure ASfast @19:47:00.84 AS.seek 0.000ml @19:47:28.56 AFV.select :carrierSolution @19:47:28.67 delay 2 seconds @19:47:30.68 AS.reconfigure ASconfig @19:47:30.73 AS.seek 1.000ml @19:48:29.35 delay 1 second @19:48:30.36 AFV.select :holdingCoil @19:48:30.47 delay 1 second @19:48:31.48 AS.reconfigure ASfast @19:48:31.53 AS.seek 0.000ml @19:48:59.26 ARV.dial between carrier and air1 @19:48:59.68 ATV.dial :air3 @19:49:00.62 AS.reconfigure ASslow1 @19:49:00.67 AS.seek 0.010ml @19:49:03.02 delay 2 seconds @19:49:05.03 ATV.dial :bleach @19:49:05.42 AS.seek 0.810ml @19:51:48.90 delay 3 seconds @19:51:51.91 ATV.dial :air3 @19:51:52.25 delay 3 seconds @19:51:55.31 AS.seek 0.835ml @19:52:00.67 delay 3 seconds @19:52:03.68 ATV.dial :bleach @19:52:04.06 AS.seek 0.820ml @19:52:07.37 delay 3 seconds @19:52:10.38 ATV.dial PCR @19:52:10.73 AS.reconfigure ASconfig @19:52:10.77 AS.seek empty @19:52:58.87 PCR.program :bleachProfile @19:52:58.94 PCR program should complete in < 2:45 @19:52:58.95 PCR.run 10:00 @19:55:57.16 PCR.off @19:55:57.20 AFV.select :carrierSolution @19:55:57.86 delay 2 seconds @19:55:59.87 AS.seek 1.000ml @19:56:58.52 delay 1 second @19:56:59.53 AFV.select :holdingCoil @19:56:59.79 delay 1 second @19:57:00.80 AS.reconfigure ASfast @19:57:00.85 AS.seek 0.000ml @19:57:28.60 delay 3 seconds @19:57:31.61 AFV.select :carrierSolution @19:57:31.71 delay 2 seconds @19:57:33.72 AS.reconfigure ASconfig @19:57:33.77 AS.seek 1.000ml @19:58:32.39 delay 1 second @19:58:33.40 AFV.select :holdingCoil @19:58:33.50 delay 1 second @19:58:34.51 AS.reconfigure ASfast @19:58:34.56 AS.seek 0.000ml @19:59:02.26 delay 3 seconds @19:59:05.27 AFV.select :carrierSolution @19:59:05.37 delay 2 seconds @19:59:07.38 AS.reconfigure ASconfig @19:59:07.43 AS.seek 1.000ml @20:00:06.04 delay 1 second @20:00:07.05 AFV.select :holdingCoil @20:00:07.16 delay 1 second @20:00:08.17 AS.reconfigure ASfast @20:00:08.22 AS.seek 0.000ml @20:00:35.93 delay 3 seconds @20:00:38.94 AFV.select :carrierSolution @20:00:39.04 delay 2 seconds @20:00:41.05 AS.reconfigure ASconfig @20:00:41.10 AS.seek 1.000ml @20:01:39.71 delay 1 second @20:01:40.72 AFV.select :holdingCoil @20:01:40.82 delay 1 second @20:01:41.83 AS.reconfigure ASfast @20:01:41.88 AS.seek 0.000ml @20:02:09.62 delay 3 seconds @20:02:12.63 AFV.select :carrierSolution @20:02:12.74 delay 2 seconds @20:02:14.75 AS.reconfigure ASconfig @20:02:14.80 AS.seek 1.000ml @20:03:13.41 delay 1 second @20:03:14.42 AFV.select :holdingCoil @20:03:14.52 delay 1 second @20:03:15.53 AS.reconfigure ASnorm @20:03:15.58 AS.seek 0.000ml @20:04:07.08 Setting up PCR for IPCap -- writing /var/log/esp/IPCap14jan2220h250ml.pcr @20:04:07.09 Building PCR Slug from ASV with MM1 mix, PP2 primer @20:04:07.11 Priming with carrier @20:04:07.12 ATV.dial ARV @20:04:07.64 ARV.dial :carrier @20:04:07.94 AS.seek 0.500ml @20:04:33.88 AFV.select :carrierSolution @20:04:33.98 delay 2 seconds @20:04:35.99 AS.reconfigure ASconfig @20:04:36.04 AS.seek 1.000ml @20:05:05.49 delay 1 second @20:05:06.50 AFV.select :holdingCoil @20:05:06.60 delay 1 second @20:05:07.61 ATV.dial :waste @20:05:08.98 AS.reconfigure ASnorm @20:05:09.03 AS.seek empty @20:06:00.53 AFV.select :carrierSolution @20:06:00.63 delay 2 seconds @20:06:02.64 AS.reconfigure ASconfig @20:06:02.68 AS.seek 0.500ml @20:06:32.19 delay 1 second @20:06:33.20 AFV.select :holdingCoil @20:06:33.30 Building Slug @20:06:33.31 ATV.dial :ARV @20:06:34.57 delay 2 seconds @20:06:36.58 ARV.dial :air1 @20:06:36.92 delay 2 seconds @20:06:38.98 AS.reconfigure ASslow2 @20:06:39.03 AS.seek 0.506ml @20:06:45.38 delay 2 seconds @20:06:47.39 ARV.dial :MM1 @20:06:47.88 delay 2 seconds @20:06:49.89 Adding MM1 @20:06:49.96 AS.seek 0.524ml @20:07:08.51 delay 25 seconds @20:07:33.52 ARV.dial :PP2 @20:07:33.88 Adding PP2 @20:07:33.89 delay 2 seconds @20:07:35.95 AS.seek 0.530ml @20:07:42.30 delay 10 seconds @20:07:52.31 ARV.dial :air2 @20:07:52.74 adding ARV air @20:07:52.75 delay 2 seconds @20:07:54.81 AS.seek 0.536ml @20:08:01.29 delay 5 seconds @20:08:06.30 ARV.dial :carrier @20:08:07.33 delay 7 seconds @20:08:14.39 AS.seek 0.594ml @20:09:13.69 delay 5 seconds @20:09:18.70 ATV.dial :ASV @20:09:19.02 delay 2 seconds @20:09:21.08 AS.seek 0.600ml @20:09:27.41 delay 4 seconds @20:09:31.42 ATV.dial ARV @20:09:31.77 delay 2 seconds @20:09:33.78 ARV.dial :carrier @20:09:33.79 delay 2 seconds @20:09:35.86 AS.seek 0.650ml @20:10:27.17 delay 4 seconds @20:10:31.18 ATV.dial PCR @20:10:31.69 ASV.dial :waste1 @20:10:31.70 ARV.dial :air1 @20:10:32.06 Positioning Slug by 0.324ml @20:10:32.13 AS.seek 0.326ml @20:16:02.83 delay 5 seconds @20:16:07.84 ATV.dial ARV @20:16:08.36 ASV.dial :PCRlock @20:16:08.86 delay 5 seconds @20:16:13.87 Running IPCap Profile59C thermocycling @20:16:13.88 PCR.program Profile59C @20:16:14.00 Profile59C should complete in < 1:39:08 @20:16:14.01 PCR.run 2:08:55 @21:41:15.10 PCR.off @21:41:15.64 PCR for IPCap completed -- closed /var/log/esp/IPCap14jan2220h250ml.pcr @21:41:15.70 Cleaning PCR @21:41:15.71 AFV.select :carrierSolution @21:41:15.82 delay 2 seconds @21:41:17.83 AS.reconfigure ASconfig @21:41:17.88 AS.seek 0.500ml @21:41:28.32 delay 1 second @21:41:29.33 AFV.select :holdingCoil @21:41:29.43 ASV.dial :waste1 @21:41:29.93 ATV.dial :air3 @21:41:30.87 AS.reconfigure ASslow1 @21:41:30.92 AS.seek 0.510ml @21:41:33.24 delay 2 seconds @21:41:35.25 ATV.dial :bleach @21:41:35.63 AS.seek 0.810ml @21:42:37.06 delay 3 seconds @21:42:40.07 ATV.dial :air3 @21:42:40.41 delay 3 seconds @21:42:43.47 AS.seek 0.835ml @21:42:48.83 delay 3 seconds @21:42:51.84 ATV.dial :bleach @21:42:52.22 AS.seek 0.820ml @21:42:55.52 delay 3 seconds @21:42:58.53 ARV.dial :waste @21:42:59.03 delay 3 seconds @21:43:02.04 ATV.dial :ARV @21:43:02.79 AS.reconfigure ASconfig @21:43:02.83 AS.seek 0.470ml @21:43:23.52 AFV.select :carrierSolution @21:43:23.63 delay 2 seconds @21:43:25.64 AS.seek 1.000ml @21:43:56.89 delay 1 second @21:43:57.90 AFV.select :holdingCoil @21:43:58.00 delay 1 second @21:43:59.01 AS.reconfigure ASfast @21:43:59.06 AS.seek 0.000ml @21:44:26.80 AFV.select :carrierSolution @21:44:26.91 delay 2 seconds @21:44:28.92 AS.reconfigure ASconfig @21:44:28.97 AS.seek 1.000ml @21:45:27.59 delay 1 second @21:45:28.60 AFV.select :holdingCoil @21:45:28.70 delay 1 second @21:45:29.71 AS.reconfigure ASfast @21:45:29.78 AS.seek 0.000ml @21:45:57.53 AFV.select :carrierSolution @21:45:57.64 delay 2 seconds @21:45:59.65 AS.reconfigure ASconfig @21:45:59.70 AS.seek 1.000ml @21:46:58.26 delay 1 second @21:46:59.27 AFV.select :holdingCoil @21:46:59.38 delay 1 second @21:47:00.39 AS.reconfigure ASfast @21:47:00.44 AS.seek 0.000ml @21:47:28.16 ARV.dial between carrier and air1 @21:47:28.59 ATV.dial :air3 @21:47:29.53 AS.reconfigure ASslow1 @21:47:29.58 AS.seek 0.010ml @21:47:31.95 delay 2 seconds @21:47:33.96 ATV.dial :bleach @21:47:34.34 AS.seek 0.810ml @21:50:18.32 delay 3 seconds @21:50:21.33 ATV.dial :air3 @21:50:21.67 delay 3 seconds @21:50:24.73 AS.seek 0.835ml @21:50:30.07 delay 3 seconds @21:50:33.08 ATV.dial :bleach @21:50:33.47 AS.seek 0.820ml @21:50:36.78 delay 3 seconds @21:50:39.79 ATV.dial PCR @21:50:40.13 AS.reconfigure ASconfig @21:50:40.19 AS.seek empty @21:51:28.37 PCR.program :bleachProfile @21:51:28.44 PCR program should complete in < 2:45 @21:51:28.45 PCR.run 10:00 @21:54:26.65 PCR.off @21:54:26.68 AFV.select :carrierSolution @21:54:26.78 delay 2 seconds @21:54:28.79 AS.seek 1.000ml @21:55:27.42 delay 1 second @21:55:28.43 AFV.select :holdingCoil @21:55:28.53 delay 1 second @21:55:29.54 AS.reconfigure ASfast @21:55:29.59 AS.seek 0.000ml @21:55:57.33 delay 3 seconds @21:56:00.34 AFV.select :carrierSolution @21:56:00.44 delay 2 seconds @21:56:02.45 AS.reconfigure ASconfig @21:56:02.49 AS.seek 1.000ml @21:57:01.11 delay 1 second @21:57:02.12 AFV.select :holdingCoil @21:57:02.22 delay 1 second @21:57:03.23 AS.reconfigure ASfast @21:57:03.27 AS.seek 0.000ml @21:57:31.00 delay 3 seconds @21:57:34.01 AFV.select :carrierSolution @21:57:34.11 delay 2 seconds @21:57:36.12 AS.reconfigure ASconfig @21:57:36.17 AS.seek 1.000ml @21:58:34.76 delay 1 second @21:58:35.77 AFV.select :holdingCoil @21:58:35.88 delay 1 second @21:58:36.93 AS.reconfigure ASfast @21:58:37.02 AS.seek 0.000ml @21:59:04.79 delay 3 seconds @21:59:07.80 AFV.select :carrierSolution @21:59:07.90 delay 2 seconds @21:59:09.91 AS.reconfigure ASconfig @21:59:09.96 AS.seek 1.000ml @22:00:08.57 delay 1 second @22:00:09.58 AFV.select :holdingCoil @22:00:09.68 delay 1 second @22:00:10.69 AS.reconfigure ASfast @22:00:10.74 AS.seek 0.000ml @22:00:38.49 delay 3 seconds @22:00:41.50 AFV.select :carrierSolution @22:00:41.61 delay 2 seconds @22:00:43.62 AS.reconfigure ASconfig @22:00:43.67 AS.seek 1.000ml @22:01:42.29 delay 1 second @22:01:43.30 AFV.select :holdingCoil @22:01:43.40 delay 1 second @22:01:44.41 AS.reconfigure ASnorm @22:01:44.46 AS.seek 0.000ml @22:02:35.91 Setting up PCR for IPCem -- writing /var/log/esp/IPCem14jan2222h250ml.pcr @22:02:35.92 Building PCR Slug from ASV with MM3 mix, PP5 primer @22:02:35.94 Priming with carrier @22:02:35.96 ATV.dial ARV @22:02:36.49 ARV.dial :carrier @22:02:36.79 AS.seek 0.500ml @22:03:02.72 AFV.select :carrierSolution @22:03:02.83 delay 2 seconds @22:03:04.84 AS.reconfigure ASconfig @22:03:04.89 AS.seek 1.000ml @22:03:34.34 delay 1 second @22:03:35.35 AFV.select :holdingCoil @22:03:35.45 delay 1 second @22:03:36.46 ATV.dial :waste @22:03:37.71 AS.reconfigure ASnorm @22:03:37.76 AS.seek empty @22:04:29.25 AFV.select :carrierSolution @22:04:29.36 delay 2 seconds @22:04:31.37 AS.reconfigure ASconfig @22:04:31.41 AS.seek 0.500ml @22:05:00.90 delay 1 second @22:05:01.91 AFV.select :holdingCoil @22:05:02.01 Building Slug @22:05:02.02 ATV.dial :ARV @22:05:03.26 delay 2 seconds @22:05:05.27 ARV.dial :air1 @22:05:05.61 delay 2 seconds @22:05:07.67 AS.reconfigure ASslow2 @22:05:07.72 AS.seek 0.506ml @22:05:14.11 delay 2 seconds @22:05:16.12 ARV.dial :MM3 @22:05:17.00 delay 2 seconds @22:05:19.01 Adding MM3 @22:05:19.08 AS.seek 0.524ml @22:05:37.76 delay 25 seconds @22:06:02.78 ARV.dial :PP5 @22:06:03.22 Adding PP5 @22:06:03.24 delay 2 seconds @22:06:05.29 AS.seek 0.530ml @22:06:11.60 delay 10 seconds @22:06:21.61 ARV.dial :air3 @22:06:21.96 adding ARV air @22:06:21.97 delay 2 seconds @22:06:24.03 AS.seek 0.536ml @22:06:30.30 delay 5 seconds @22:06:35.31 ARV.dial :carrier @22:06:36.35 delay 7 seconds @22:06:43.41 AS.seek 0.594ml @22:07:42.71 delay 5 seconds @22:07:47.72 ATV.dial :ASV @22:07:48.16 delay 2 seconds @22:07:50.22 AS.seek 0.600ml @22:07:56.47 delay 4 seconds @22:08:00.48 ATV.dial ARV @22:08:00.81 delay 2 seconds @22:08:02.82 ARV.dial :carrier @22:08:02.83 delay 2 seconds @22:08:04.89 AS.seek 0.650ml @22:08:56.08 delay 4 seconds @22:09:00.60 ATV.dial PCR @22:09:01.11 ASV.dial :waste1 @22:09:01.12 ARV.dial :air1 @22:09:01.48 Positioning Slug by 0.324ml @22:09:01.55 AS.seek 0.326ml @22:14:32.25 delay 5 seconds @22:14:37.26 ATV.dial ARV @22:14:37.79 ASV.dial :PCRlock @22:14:38.31 delay 5 seconds @22:14:43.31 Running IPCem ProfileEM59C thermocycling @22:14:43.32 PCR.program ProfileEM59C @22:14:43.45 ProfileEM59C should complete in < 2:11:38 @22:14:43.46 PCR.run 2:49:32.5 @00:12:54.93PST23-Jan-14 PCR.off @00:12:55.46 PCR for IPCem completed -- closed /var/log/esp/IPCem14jan2222h250ml.pcr @00:12:55.48 Full MFB Decontamination @00:12:55.49 Decon extraction column and decon AIV @00:12:55.52 SPE.hold 2:46:40,55.0C @00:12:55.55 ASV.dial :SPEdelivery @00:12:56.52 delay 20 seconds @00:13:16.53 AFV.select :carrierSolution @00:13:16.63 delay 2 seconds @00:13:18.64 AS.reconfigure ASconfig @00:13:18.69 AS.seek 0.500ml @00:13:29.16 delay 1 second @00:13:30.17 AFV.select :holdingCoil @00:13:30.27 ATV.dial :air1 @00:13:31.80 AS.reconfigure ASslow1 @00:13:31.85 AS.seek 0.520ml @00:13:36.22 ATV.dial :ASV @00:13:37.52 ASV.dial :SPErecovery @00:13:38.52 AS.seek 0.620ml @00:13:59.19 ATV.dial :waste @00:14:00.78 AS.reconfigure ASnorm @00:14:00.83 AS.seek empty @00:14:32.87 AFV.select :holdingCoil @00:14:32.88 ATV.dial :waste @00:14:32.90 AS.reconfigure ASconfig @00:14:32.96 AS.seek 0.000ml @00:14:33.27 AFV.select :carrierSolution @00:14:33.38 delay 2 seconds @00:14:35.39 AS.seek 0.500ml @00:15:04.84 delay 1 second @00:15:05.85 AFV.select :holdingCoil @00:15:05.95 ATV.dial :air3 @00:15:07.08 AS.reconfigure ASslow1 @00:15:07.13 AS.seek 0.520ml @00:15:11.48 ATV.dial :air3 @00:15:11.56 AS.seek 0.530ml @00:15:13.87 delay 2 seconds @00:15:15.88 ATV.dial :bleach @00:15:16.27 AS.seek 0.710ml @00:15:53.23 delay 3 seconds @00:15:56.24 ATV.dial :air3 @00:15:56.68 delay 3 seconds @00:15:59.74 AS.seek 0.735ml @00:16:05.12 delay 3 seconds @00:16:08.13 ATV.dial :bleach @00:16:08.52 AS.seek 0.720ml @00:16:11.84 delay 3 seconds @00:16:14.85 ATV.dial ASV @00:16:15.73 ASV.dial :SPErecovery @00:16:15.81 AS.seek 0.365ml @00:17:28.46 ATV.dial :waste @00:17:29.93 AS.reconfigure ASnorm @00:17:29.98 AS.seek empty @00:17:49.04 ATV.dial :air3 @00:17:49.62 AS.reconfigure ASslow1 @00:17:49.67 AS.seek 0.009ml @00:17:52.04 delay 2 seconds @00:17:54.05 ATV.dial :bleach @00:17:54.42 AS.seek 0.579ml @00:19:50.97 delay 3 seconds @00:19:53.98 ATV.dial :air3 @00:19:54.32 delay 3 seconds @00:19:57.38 AS.seek 0.604ml @00:20:02.74 delay 3 seconds @00:20:05.75 ATV.dial :bleach @00:20:06.13 AS.seek 0.589ml @00:20:09.44 delay 3 seconds @00:20:12.45 ATV.dial :ASV @00:20:13.33 ASV.dial :wasteR @00:20:13.67 AS.seek 0.000ml @00:22:14.16 delay 1:00 @00:23:14.18 ATV.dial ASV @00:23:14.19 AFV.select :carrierSolution @00:23:14.30 delay 2 seconds @00:23:16.31 AS.reconfigure ASconfig @00:23:16.36 AS.seek 1.000ml @00:24:14.96 delay 1 second @00:24:15.97 AFV.select :holdingCoil @00:24:16.07 delay 1 second @00:24:17.08 ASV.dial :wasteR @00:24:17.09 AS.reconfigure ASslow1 @00:24:17.14 AS.seek 0.000ml @00:27:41.37 delay 10 seconds @00:27:51.38 ATV.dial :air3 @00:27:52.50 AS.seek 0.010ml @00:27:54.75 delay 2 seconds @00:27:56.76 ATV.dial :bleach @00:27:57.14 AS.seek 0.580ml @00:29:53.65 delay 3 seconds @00:29:56.66 ATV.dial :air3 @00:29:57.09 delay 3 seconds @00:30:00.15 AS.seek 0.605ml @00:30:05.51 delay 3 seconds @00:30:08.52 ATV.dial :bleach @00:30:09.01 AS.seek 0.590ml @00:30:12.31 delay 3 seconds @00:30:15.32 ATV.dial ASV @00:30:16.21 ASV.dial :SPEdelivery @00:30:16.55 AS.seek 0.000ml @00:32:17.10 delay 1:00 @00:33:17.11 AFV.select :carrierSolution @00:33:17.21 delay 2 seconds @00:33:19.22 AS.reconfigure ASconfig @00:33:19.27 AS.seek 1.000ml @00:34:17.88 delay 1 second @00:34:18.89 AFV.select :holdingCoil @00:34:18.99 delay 1 second @00:34:20.00 ATV.dial ASV @00:34:20.01 ASV.dial :SPEdelivery @00:34:20.03 AS.reconfigure ASslow1 @00:34:20.08 AS.seek 0.000ml @00:37:44.34 delay 10 seconds @00:37:54.35 ATV.dial :air2 @00:37:55.08 AS.reconfigure ASslow0 @00:37:55.13 AS.seek 0.800ml @00:39:17.05 delay 3 seconds @00:39:20.06 ATV.dial :ASV @00:39:20.77 ASV.dial :SPEdelivery @00:39:20.79 AS.reconfigure ASslow1 @00:39:20.84 AS.seek 0.030ml @00:41:58.14 delay 20 seconds @00:42:18.15 ATV.dial :waste,via => air3 @00:42:19.58 AS.reconfigure ASconfig @00:42:19.62 AS.seek empty @00:42:21.70 SPE.coast @00:42:21.78 Removing Bleach from Mixing Coil @00:42:21.79 AFV.select :carrierSolution @00:42:21.89 delay 2 seconds @00:42:23.90 AS.seek 0.500ml @00:42:53.41 delay 1 second @00:42:54.42 AFV.select :holdingCoil @00:42:54.52 ATV.dial :ASV @00:42:56.06 ASV.dial :SPErecovery @00:42:56.78 AS.reconfigure ASslow1 @00:42:56.87 AS.seek 0.730ml @00:43:44.10 ASV.dial :wasteD @00:43:44.63 AS.reconfigure ASnorm @00:43:44.68 AS.seek 0.000ml @00:44:22.29 AFV.select :carrierSolution @00:44:22.40 delay 2 seconds @00:44:24.41 AS.reconfigure ASconfig @00:44:24.46 AS.seek 0.500ml @00:44:53.91 delay 1 second @00:44:54.92 AFV.select :holdingCoil @00:44:55.03 ATV.dial ASV @00:44:55.04 ASV.dial :SPErecovery @00:44:56.02 AS.reconfigure ASslow0 @00:44:56.07 AS.seek 0.300ml @00:45:16.75 ATV.dial :air2 @00:45:17.48 AS.reconfigure ASslow1 @00:45:17.53 AS.seek 0.320ml @00:45:21.92 ATV.dial ASV @00:45:23.32 AS.reconfigure ASslow0 @00:45:23.37 AS.seek 0.570ml @00:45:49.11 ASV.dial :wasteD @00:45:49.62 AS.reconfigure ASnorm @00:45:49.67 AS.seek 0.000ml @00:46:19.14 AFV.select :carrierSolution @00:46:19.25 delay 2 seconds @00:46:21.26 AS.reconfigure ASconfig @00:46:21.31 AS.seek 0.500ml @00:46:50.77 delay 1 second @00:46:51.78 AFV.select :holdingCoil @00:46:51.89 ATV.dial ASV @00:46:51.90 ASV.dial :SPErecovery @00:46:52.61 AS.reconfigure ASslow0 @00:46:52.66 AS.seek 0.300ml @00:47:13.36 ATV.dial :air2 @00:47:14.11 AS.reconfigure ASslow1 @00:47:14.16 AS.seek 0.320ml @00:47:18.51 ATV.dial ASV @00:47:19.38 AS.reconfigure ASslow0 @00:47:19.43 AS.seek 0.570ml @00:47:45.22 ASV.dial :wasteD @00:47:45.88 AS.reconfigure ASnorm @00:47:45.93 AS.seek 0.000ml @00:48:15.36 ATV.dial :air2 @00:48:16.07 AS.reconfigure ASslow0 @00:48:16.12 AS.seek 0.300ml @00:48:47.03 ATV.dial :ASV @00:48:47.84 ASV.dial :wasteD @00:48:47.86 AS.reconfigure ASconfig @00:48:47.91 AS.seek 0.050ml @00:49:02.89 ATV.dial :waste @00:49:04.33 AS.seek 0.000ml @00:49:07.50 ASV.dial :SPEdelivery @00:49:08.51 deconLysateINT @00:49:08.52 ATV.dial :air3 @00:49:09.19 AS.reconfigure ASslow1 @00:49:09.24 AS.seek 0.010ml @00:49:11.60 delay 2 seconds @00:49:13.61 ATV.dial :bleach @00:49:14.00 AS.seek 0.960ml @00:52:28.00 delay 3 seconds @00:52:31.01 ATV.dial :air3 @00:52:31.35 delay 3 seconds @00:52:34.41 AS.seek 0.985ml @00:52:39.78 delay 3 seconds @00:52:42.79 ATV.dial :bleach @00:52:43.18 AS.seek 0.970ml @00:52:46.49 delay 3 seconds @00:52:49.50 ATV.dial :AIV @00:52:50.58 AIV.dial :coilToMFB @00:52:50.59 AWV.select :waste @00:52:50.61 AFV.select :carrierSolution @00:52:50.72 delay 2 seconds @00:52:52.73 AS.reconfigure ASconfig @00:52:52.78 AS.seek 1.000ml @00:52:54.86 delay 1 second @00:52:55.87 AFV.select :holdingCoil @00:52:55.97 delay 1 second @00:52:56.98 AS.reconfigure ASslow0 @00:52:57.03 AS.seek empty @00:54:39.44 AFV.select :carrierSolution @00:54:39.55 delay 2 seconds @00:54:41.56 AS.reconfigure ASconfig @00:54:41.61 AS.seek 1.000ml @00:55:40.26 delay 1 second @00:55:41.27 AFV.select :holdingCoil @00:55:41.37 delay 1 second @00:55:42.38 AS.reconfigure ASslow0 @00:55:42.43 AS.seek empty @00:57:24.82 AFV.select :carrierSolution @00:57:24.93 delay 2 seconds @00:57:26.94 AS.reconfigure ASconfig @00:57:26.99 AS.seek 1.000ml @00:58:25.64 delay 1 second @00:58:26.65 AFV.select :holdingCoil @00:58:26.75 delay 1 second @00:58:27.76 AS.reconfigure ASslow0 @00:58:27.81 AS.seek empty @01:00:10.25 ATV.dial :air2 @01:00:10.92 AS.reconfigure ASslow1 @01:00:10.97 AS.seek 0.009ml @01:00:13.31 AS.reconfigure ASslow0 @01:00:13.36 AS.seek 1.000ml @01:01:54.70 ATV.dial AIV @01:01:55.24 AS.seek 0.020ml @01:03:35.54 ATV.dial :waste @01:03:36.91 AS.seek 0.000ml @01:03:39.21 AWV.select :air @01:03:39.36 Cleaning PCR @01:03:39.37 AFV.select :carrierSolution @01:03:39.48 delay 2 seconds @01:03:41.49 AS.reconfigure ASconfig @01:03:41.54 AS.seek 0.500ml @01:04:10.97 delay 1 second @01:04:11.98 AFV.select :holdingCoil @01:04:12.08 ASV.dial :waste1 @01:04:12.55 ATV.dial :air3 @01:04:13.21 AS.reconfigure ASslow1 @01:04:13.25 AS.seek 0.510ml @01:04:15.58 delay 2 seconds @01:04:17.59 ATV.dial :bleach @01:04:17.98 AS.seek 0.810ml @01:05:19.35 delay 3 seconds @01:05:22.36 ATV.dial :air3 @01:05:22.69 delay 3 seconds @01:05:25.75 AS.seek 0.835ml @01:05:31.14 delay 3 seconds @01:05:34.15 ATV.dial :bleach @01:05:34.54 AS.seek 0.820ml @01:05:37.86 delay 3 seconds @01:05:40.87 ARV.dial :waste @01:05:41.49 delay 3 seconds @01:05:44.50 ATV.dial :ARV @01:05:45.26 AS.reconfigure ASconfig @01:05:45.31 AS.seek 0.470ml @01:06:06.00 AFV.select :carrierSolution @01:06:06.11 delay 2 seconds @01:06:08.12 AS.seek 1.000ml @01:06:39.89 delay 1 second @01:06:40.90 AFV.select :holdingCoil @01:06:41.01 delay 1 second @01:06:42.02 AS.reconfigure ASfast @01:06:42.07 AS.seek 0.000ml @01:07:09.81 AFV.select :carrierSolution @01:07:09.92 delay 2 seconds @01:07:11.93 AS.reconfigure ASconfig @01:07:11.98 AS.seek 1.000ml @01:08:10.59 delay 1 second @01:08:11.60 AFV.select :holdingCoil @01:08:11.70 delay 1 second @01:08:12.71 AS.reconfigure ASfast @01:08:12.76 AS.seek 0.000ml @01:08:40.51 AFV.select :carrierSolution @01:08:40.62 delay 2 seconds @01:08:42.63 AS.reconfigure ASconfig @01:08:42.68 AS.seek 1.000ml @01:09:41.29 delay 1 second @01:09:42.30 AFV.select :holdingCoil @01:09:42.40 delay 1 second @01:09:43.41 AS.reconfigure ASfast @01:09:43.46 AS.seek 0.000ml @01:10:11.20 ARV.dial between carrier and air1 @01:10:11.73 ATV.dial :air3 @01:10:12.68 AS.reconfigure ASslow1 @01:10:12.73 AS.seek 0.010ml @01:10:15.09 delay 2 seconds @01:10:17.10 ATV.dial :bleach @01:10:17.48 AS.seek 0.810ml @01:13:00.89 delay 3 seconds @01:13:03.90 ATV.dial :air3 @01:13:04.24 delay 3 seconds @01:13:07.30 AS.seek 0.835ml @01:13:12.66 delay 3 seconds @01:13:15.67 ATV.dial :bleach @01:13:16.05 AS.seek 0.820ml @01:13:19.38 delay 3 seconds @01:13:22.39 ATV.dial PCR @01:13:22.73 AS.reconfigure ASconfig @01:13:22.78 AS.seek empty @01:14:10.90 PCR.program :bleachProfile @01:14:10.97 PCR program should complete in < 2:45 @01:14:10.98 PCR.run 10:00 @01:17:12.21 PCR.off @01:17:12.24 AFV.select :carrierSolution @01:17:12.34 delay 2 seconds @01:17:14.35 AS.seek 1.000ml @01:18:13.00 delay 1 second @01:18:14.01 AFV.select :holdingCoil @01:18:14.11 delay 1 second @01:18:15.12 AS.reconfigure ASfast @01:18:15.17 AS.seek 0.000ml @01:18:42.94 delay 3 seconds @01:18:45.95 AFV.select :carrierSolution @01:18:46.05 delay 2 seconds @01:18:48.06 AS.reconfigure ASconfig @01:18:48.11 AS.seek 1.000ml @01:19:46.73 delay 1 second @01:19:47.74 AFV.select :holdingCoil @01:19:47.84 delay 1 second @01:19:48.85 AS.reconfigure ASfast @01:19:48.89 AS.seek 0.000ml @01:20:16.59 delay 3 seconds @01:20:19.60 AFV.select :carrierSolution @01:20:19.70 delay 2 seconds @01:20:21.71 AS.reconfigure ASconfig @01:20:21.76 AS.seek 1.000ml @01:21:20.37 delay 1 second @01:21:21.38 AFV.select :holdingCoil @01:21:21.48 delay 1 second @01:21:22.49 AS.reconfigure ASfast @01:21:22.54 AS.seek 0.000ml @01:21:50.29 delay 3 seconds @01:21:53.30 AFV.select :carrierSolution @01:21:53.40 delay 2 seconds @01:21:55.41 AS.reconfigure ASconfig @01:21:55.46 AS.seek 1.000ml @01:22:54.09 delay 1 second @01:22:55.10 AFV.select :holdingCoil @01:22:55.20 delay 1 second @01:22:56.21 AS.reconfigure ASfast @01:22:56.26 AS.seek 0.000ml @01:23:23.98 delay 3 seconds @01:23:26.99 AFV.select :carrierSolution @01:23:27.10 delay 2 seconds @01:23:29.11 AS.reconfigure ASconfig @01:23:29.16 AS.seek 1.000ml @01:24:27.75 delay 1 second @01:24:28.76 AFV.select :holdingCoil @01:24:28.86 delay 1 second @01:24:29.87 AS.reconfigure ASnorm @01:24:29.92 AS.seek 0.000ml @01:25:21.40 AFV.select :carrierSolution @01:25:21.50 delay 2 seconds @01:25:23.51 AS.reconfigure ASconfig @01:25:23.56 AS.seek 1.000ml @01:26:22.19 delay 1 second @01:26:23.20 AFV.select :holdingCoil @01:26:23.30 delay 1 second @01:26:24.31 ATV.dial :waste @01:26:25.29 AS.reconfigure ASnorm @01:26:25.34 AS.seek 0.500ml @01:26:51.28 ATV.dial :waste2 @01:26:52.37 AS.seek 0.000ml @01:27:18.31
Cleaning PCR AFV.select :carrierSolution @01:27:18.42 delay 2 seconds @01:27:20.43 AS.reconfigure ASconfig @01:27:20.47 AS.seek 0.500ml @01:27:49.92 delay 1 second @01:27:50.93 AFV.select :holdingCoil @01:27:51.03 ASV.dial :waste1 @01:27:51.04 ATV.dial :air3 @01:27:52.61 AS.reconfigure ASslow1 @01:27:52.65 AS.seek 0.510ml @01:27:55.00 delay 2 seconds @01:27:57.01 ATV.dial :bleach @01:27:57.39 AS.seek 0.810ml @01:28:58.90 delay 3 seconds @01:29:01.91 ATV.dial :air3 @01:29:02.35 delay 3 seconds @01:29:05.41 AS.seek 0.835ml @01:29:10.74 delay 3 seconds @01:29:13.75 ATV.dial :bleach @01:29:14.23 AS.seek 0.820ml @01:29:17.56 delay 3 seconds @01:29:21.05 ARV.dial :waste @01:29:21.45 delay 3 seconds @01:29:24.46 ATV.dial :ARV @01:29:25.33 AS.reconfigure ASconfig @01:29:25.37 AS.seek 0.470ml @01:29:46.17 AFV.select :carrierSolution @01:29:46.28 delay 2 seconds @01:29:48.30 AS.seek 1.000ml @01:30:19.55 delay 1 second @01:30:20.56 AFV.select :holdingCoil @01:30:20.66 delay 1 second @01:30:21.68 AS.reconfigure ASfast @01:30:21.73 AS.seek 0.000ml @01:30:49.45 AFV.select :carrierSolution @01:30:49.56 delay 2 seconds @01:30:51.58 AS.reconfigure ASconfig @01:30:51.63 AS.seek 1.000ml @01:31:50.23 delay 1 second @01:31:51.24 AFV.select :holdingCoil @01:31:51.34 delay 1 second @01:31:52.36 AS.reconfigure ASfast @01:31:52.41 AS.seek 0.000ml @01:32:20.12 AFV.select :carrierSolution @01:32:20.22 delay 2 seconds @01:32:22.23 AS.reconfigure ASconfig @01:32:22.28 AS.seek 1.000ml @01:33:20.82 delay 1 second @01:33:21.83 AFV.select :holdingCoil @01:33:21.93 delay 1 second @01:33:22.94 AS.reconfigure ASfast @01:33:22.99 AS.seek 0.000ml @01:33:50.71 ARV.dial between carrier and air1 @01:33:51.14 ATV.dial :air3 @01:33:52.08 AS.reconfigure ASslow1 @01:33:52.12 AS.seek 0.010ml @01:33:54.46 delay 2 seconds @01:33:56.47 ATV.dial :bleach @01:33:56.86 AS.seek 0.810ml @01:36:40.27 delay 3 seconds @01:36:43.28 ATV.dial :air3 @01:36:43.61 delay 3 seconds @01:36:46.68 AS.seek 0.835ml @01:36:52.05 delay 3 seconds @01:36:55.06 ATV.dial :bleach @01:36:55.45 AS.seek 0.820ml @01:36:58.75 delay 3 seconds @01:37:01.76 ATV.dial PCR @01:37:02.11 AS.reconfigure ASconfig @01:37:02.15 AS.seek empty @01:37:50.25 PCR.program :bleachProfile @01:37:50.31 PCR program should complete in < 2:45 @01:37:50.32 PCR.run 10:00 @01:40:51.54 PCR.off @01:40:51.57 AFV.select :carrierSolution @01:40:51.67 delay 2 seconds @01:40:53.69 AS.seek 1.000ml @01:41:52.36 delay 1 second @01:41:53.37 AFV.select :holdingCoil @01:41:53.47 delay 1 second @01:41:54.48 AS.reconfigure ASfast @01:41:54.53 AS.seek 0.000ml @01:42:22.24 delay 3 seconds @01:42:25.25 AFV.select :carrierSolution @01:42:25.35 delay 2 seconds @01:42:27.37 AS.reconfigure ASconfig @01:42:27.42 AS.seek 1.000ml @01:43:26.02 delay 1 second @01:43:27.03 AFV.select :holdingCoil @01:43:27.13 delay 1 second @01:43:28.15 AS.reconfigure ASfast @01:43:28.20 AS.seek 0.000ml @01:43:55.90 delay 3 seconds @01:43:58.91 AFV.select :carrierSolution @01:43:59.01 delay 2 seconds @01:44:01.03 AS.reconfigure ASconfig @01:44:01.08 AS.seek 1.000ml @01:44:59.68 delay 1 second @01:45:00.69 AFV.select :holdingCoil @01:45:00.79 delay 1 second @01:45:01.81 AS.reconfigure ASfast @01:45:01.86 AS.seek 0.000ml @01:45:29.58 delay 3 seconds @01:45:32.59 AFV.select :carrierSolution @01:45:32.69 delay 2 seconds @01:45:34.71 AS.reconfigure ASconfig @01:45:34.75 AS.seek 1.000ml @01:46:33.36 delay 1 second @01:46:34.37 AFV.select :holdingCoil @01:46:34.47 delay 1 second @01:46:35.48 AS.reconfigure ASfast @01:46:35.53 AS.seek 0.000ml @01:47:03.25 delay 3 seconds @01:47:06.26 AFV.select :carrierSolution @01:47:06.36 delay 2 seconds @01:47:08.37 AS.reconfigure ASconfig @01:47:08.42 AS.seek 1.000ml @01:48:07.04 delay 1 second @01:48:08.05 AFV.select :holdingCoil @01:48:08.15 delay 1 second @01:48:09.17 AS.reconfigure ASnorm @01:48:09.22 AS.seek 0.000ml @01:49:00.71 Completed {wqPCR} begun 11:25:32 @01:49:00.75 Asleep til +0:10 @01:49:00.76 AS.stop @01:49:00.79
Sleeping until 01:49 @01:49:00.82 CC.stop @01:49:00.85 GoodNews.email "Then, in tube 2: wqPCR\n/home/esp/esp2/mission/14jan21SCCWRP.rb:53",Subject => "Asleep until 01:49" @01:49:02.54 CS.stop @01:49:02.58 PC.stop @01:49:02.62 PS.stop @01:49:02.66 Forearm.stop @01:49:02.70 Elbow.stop @01:49:02.74 SS.stop @01:49:02.82 Sleepy.power :raw,:OFF @01:49:02.83 Sleepy.power :dwarf,:OFF @01:49:02.84 Shutdown @01:49:11.04
Powering up Dwarves @01:49:15.84 Sleepy.power :raw,:ON @01:49:16.31 SPE.configure SPEconfig @01:49:16.34 AS.configure ASconfig @01:49:16.38 CC.configure CCconfig @01:49:16.42 CS.configure CSconfig @01:49:16.46 PC.configure PCconfig @01:49:16.50 PS.configure PSconfig @01:49:16.55 Forearm.configure LooseForearm @01:49:16.59 Elbow.configure LooseElbow @01:49:16.63 SC.configure SCloose @01:49:16.67 SE.configure SEconfig @01:49:16.71 SS.configure SSconfig @01:49:16.75 CH.configure CHconfig @01:49:16.78 PH.configure PHconfig @01:49:16.82 Valve.reset @01:49:17.34 PCR.off @01:49:17.38 PCR.pidtemp= 50,0.1,0,300,25,25 @01:49:17.42 PCR.caltemp= 6.6661,0.0344,0.0 @01:49:17.54 Intake.select :close @01:49:17.55 Exhaust.select :close @01:49:17.56 CTV.dial :air @01:49:17.63 PSV.dial :air @01:49:17.66
PC.reconfigure PCconfig @01:49:17.73 PC.home.seek open @01:49:17.75 CSV.dial :waste1 @01:49:18.14 PS.reconfigure PSconfig @01:49:18.18 PS.home.seek 0.000ml @01:49:18.24 SSV.dial :spit,avoiding => exhaust @01:49:19.02 CS.reconfigure CSfast @01:49:19.05 SS.reconfigure SSconfig @01:49:19.16 SS.home.seek 0.00ml @01:49:19.18 CS.home.seek 0.000ml @01:49:19.27 CC.reconfigure CCconfig @01:49:19.32 CC.home.seek open @01:49:28.34 PS.coast @01:49:28.43
CC.seek open @01:49:28.46 PC.seek open @01:49:28.63
Forearm.reconfigure LooseForearm @01:49:28.67 Forearm.home.seek retract @01:49:29.40 Hand.grip @01:49:29.43
Elbow.jog -1500 @01:49:29.96 Elbow.reconfigure LooseElbow @01:49:30.01 Elbow.home.seek clear @01:49:34.70 SE.reconfigure SEconfig @01:49:34.75 SE.home.seek down @01:49:35.34 SC.reconfigure SCsloppy @01:49:35.39 SC.home.seek initial @01:49:37.63 Forearm.seek retract @01:49:37.70 Elbow.seek hammer @01:49:38.91 Forearm.reconfigure TightForearm @01:49:38.97 Forearm.seek 2 @01:49:40.10 Forearm.seek retract @01:49:41.28 Forearm.reconfigure LooseForearm @01:49:41.71PST23-Jan-14 ===> STARTED wqPCR (phase #2) <=== @01:49:41.81 Starting mfb pcrBAC sampling at most 250ml, wcr at most 1000ml @01:49:41.83 BACfans.email "Can@01:49:41, 22.2C, 45% humidity, 14.4psia, 13.724V, 0.701A, 0.714A avg, 9.62W\nCTD data unavailable\nISUS data unavailable",Subject => "pcrBAC sampling at most 250ml, wcr at most 1000ml" @01:49:41.87
Loading sh1 puck from tube 2 into Collection Clamp @01:49:44.03 Readying the MFB @01:49:44.04 Homing MFB Syringe @01:49:44.05 AFV.select :holdingCoil @01:49:44.11
Hand.release @01:49:44.20 ATV.dial :air2 @01:49:44.96
SC.reconfigure SCloose @01:49:45.11 Elbow.reconfigure TightElbow @01:49:45.15 Elbow.seek 2 @01:49:45.33 Forearm.reconfigure TightForearm @01:49:45.38 Forearm.seek 2 + 800 counts @01:49:45.46 AS.reconfigure ASconfig @01:49:45.50 AS.home.seek home @01:49:46.48
SE.reconfigure SEpick @01:49:46.55 SE.seek beamBreak @01:49:49.03 SE.configure SEconfig @01:49:49.26 Elbow.seek 2 @01:49:49.43 Forearm.seek 2 + 300 counts @01:49:49.65 Forearm.reconfigure LooseForearm @01:49:49.70 Hand.grip @01:49:50.44 SE.seek down @01:49:50.60
Forearm.seek retract @01:49:51.58 Elbow.seek CC @01:49:52.75 SE.coast @01:49:53.13 AFV.select :carrierSolution @01:49:53.23
Elbow.reconfigure LooseElbow @01:49:53.29 delay 2 seconds @01:49:53.35
Forearm.reconfigure TightForearm @01:49:53.40 Forearm.seek CC @01:49:54.53 CC.seek closed @01:49:55.30 AS.seek 1.000ml @01:49:58.30
Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.92 @01:49:58.31 Hand.release @01:49:59.09 Forearm.reconfigure LooseForearm @01:49:59.13 Forearm.seek retract @01:50:00.12 SC.coast @01:50:00.16 collectSample 250ml w/bubble point of 55psi @01:50:00.17 Priming 7ml @01:50:00.18 Intake.select :close @01:50:00.20 Exhaust.select :close @01:50:00.22 SS.hold @01:50:00.40 Calibrating pressure and temperature sensors @01:50:00.41 CTV.dial :air @01:50:00.44 SSV.dial :spit,avoiding => exhaust @01:50:00.46 SS.reconfigure SSfast @01:50:00.51 SS.seek 5.00ml @01:50:03.59 SSV.dial CBV @01:50:03.61
CBV.dial SSV @01:50:04.94 delay 3.8 seconds @01:50:08.82 SSV.dial :spit @01:50:10.13 SS.seek 0.00ml @01:50:12.68 SSV.dial CBV @01:50:13.98 CTV.dial :intake @01:50:14.76 SSV.dial CBV @01:50:14.77
CBV.dial SSV @01:50:14.79 Allowing 55s for 7.0ml of remaining 7.0ml @01:50:14.80 Intake.select :open @01:50:16.95 Intake @27.6psia, vacuum from 20.0 to 24.0psi @01:50:16.96 SS.reconfigure forSampling @01:50:17.02 SS.seek 7.00ml @01:50:21.72 Vacuum too high -- waiting up to 40s @01:50:23.33 Intake @24.6psia, vacuum from 18.1 to 22.1psi @01:50:23.34 SS.reconfigure forSampling @01:50:23.40 SS.seek 7.00ml @01:50:24.71 Stroke completed -- waiting for pressure to equalize ... @01:50:28.29 Intake.select :close @01:50:28.44 SSV.dial :exhaust @01:50:29.20 Exhaust.select :open @01:50:29.31 SS.reconfigure SSexhale @01:50:29.36 SS.seek 0.00ml @01:50:32.87 Exhaust.select :close @01:50:32.97 Sampling 250.0ml @01:50:32.98 Intake.select :close @01:50:32.99 Exhaust.select :close @01:50:33.01 SS.reconfigure SSconfig @01:50:33.06 SS.hold @01:50:33.22 CTV.dial :intake @01:50:33.23 SSV.dial CBV @01:50:33.26
CBV.dial SSV @01:50:34.03 Allowing 185s for 25.0ml of remaining 250.0ml @01:50:34.04 Intake.select :open @01:50:36.18 Intake @27.7psia, vacuum from 20.0 to 24.0psi @01:50:36.19 SS.reconfigure forSampling @01:50:36.24 SS.seek 25.00ml @01:50:39.05 Vacuum too high -- waiting up to 40s @01:50:39.52 Intake @24.9psia, vacuum from 18.4 to 22.4psi @01:50:39.53 SS.reconfigure forSampling @01:50:39.60 SS.seek 25.00ml @01:50:42.01 Vacuum too high -- waiting up to 40s @01:50:43.13 Intake @25.8psia, vacuum from 19.3 to 23.3psi @01:50:43.14 SS.reconfigure forSampling @01:50:43.20 SS.seek 25.00ml @01:50:45.83 Vacuum too high -- waiting up to 40s @01:50:46.97 Intake @25.7psia, vacuum from 19.2 to 23.2psi @01:50:46.98 SS.reconfigure forSampling @01:50:47.05 SS.seek 25.00ml @01:50:47.07 delay 1 second @01:50:48.08 AFV.select :holdingCoil @01:50:48.18 delay 1 second @01:50:49.19 ATV.dial :waste @01:50:50.01
Vacuum too high -- waiting up to 40s @01:50:50.08 AS.reconfigure ASnorm @01:50:50.12 AS.home.seek empty @01:50:51.16
Intake @25.6psia, vacuum from 19.1 to 23.1psi @01:50:51.17 SS.reconfigure forSampling @01:50:51.22 SS.seek 25.00ml @01:50:54.85 Vacuum too high -- waiting up to 40s @01:50:55.99 Intake @25.6psia, vacuum from 19.1 to 23.1psi @01:50:56.00 SS.reconfigure forSampling @01:50:56.05 SS.seek 25.00ml @01:51:09.83 Stroke completed -- waiting for pressure to equalize ... @01:51:13.42 Intake.select :close @01:51:13.57 SSV.dial :exhaust @01:51:14.32 Exhaust.select :open @01:51:14.44 SS.reconfigure SSexhale @01:51:14.48 SS.seek 0.00ml @01:51:23.10 Exhaust.select :close @01:51:23.21 SSV.dial CBV @01:51:23.23
CBV.dial SSV @01:51:23.99 Allowing 185s for 25.0ml of remaining 225.0ml @01:51:24.00 Intake.select :open @01:51:26.15 Intake @27.4psia, vacuum from 20.0 to 24.0psi @01:51:26.16 SS.reconfigure forSampling @01:51:26.22 SS.seek 25.00ml @01:51:43.38 ATV.dial :waste @01:51:43.39 ARV.dial between carrier and air1 @01:51:43.47 ASV.dial :waste1 @01:51:46.50
Vacuum too high -- waiting up to 40s @01:51:47.18 Intake @25.1psia, vacuum from 18.6 to 22.6psi @01:51:47.19 SS.reconfigure forSampling @01:51:47.25 SS.seek 25.00ml @01:52:00.91 Stroke completed -- waiting for pressure to equalize ... @01:52:02.70 Intake.select :close @01:52:02.84 SSV.dial :exhaust @01:52:03.58 Exhaust.select :open @01:52:03.69 SS.reconfigure SSexhale @01:52:04.32 SS.seek 0.00ml @01:52:13.02 Exhaust.select :close @01:52:13.13 SSV.dial CBV @01:52:13.15
CBV.dial SSV @01:52:13.92 Allowing 185s for 25.0ml of remaining 200.0ml @01:52:13.93 Intake.select :open @01:52:16.08 Intake @27.1psia, vacuum from 20.0 to 24.0psi @01:52:16.09 SS.reconfigure forSampling @01:52:16.15 SS.seek 25.00ml @01:52:56.17 Stroke completed -- waiting for pressure to equalize ... @01:52:57.98 Intake.select :close @01:52:58.13 SSV.dial :exhaust @01:52:58.88 Exhaust.select :open @01:52:58.99 SS.reconfigure SSexhale @01:52:59.04 SS.seek 0.00ml @01:53:07.70 Exhaust.select :close @01:53:07.81 SSV.dial CBV @01:53:07.83
CBV.dial SSV @01:53:08.59 Allowing 185s for 25.0ml of remaining 175.0ml @01:53:08.61 Intake.select :open @01:53:10.75 Intake @27.0psia, vacuum from 20.0 to 24.0psi @01:53:10.76 SS.reconfigure forSampling @01:53:10.82 SS.seek 25.00ml @01:53:50.92 Stroke completed -- waiting for pressure to equalize ... @01:53:52.72 Intake.select :close @01:53:52.86 SSV.dial :exhaust @01:53:53.61 Exhaust.select :open @01:53:53.72 SS.reconfigure SSexhale @01:53:53.77 SS.seek 0.00ml @01:54:02.40 Exhaust.select :close @01:54:02.51 SSV.dial CBV @01:54:02.53
CBV.dial SSV @01:54:03.29 Allowing 185s for 25.0ml of remaining 150.0ml @01:54:03.30 Intake.select :open @01:54:05.46 Intake @26.8psia, vacuum from 20.0 to 24.0psi @01:54:05.47 SS.reconfigure forSampling @01:54:05.53 SS.seek 25.00ml @01:54:45.64 Stroke completed -- waiting for pressure to equalize ... @01:54:47.45 Intake.select :close @01:54:47.60 SSV.dial :exhaust @01:54:48.37 Exhaust.select :open @01:54:48.48 SS.reconfigure SSexhale @01:54:48.53 SS.seek 0.00ml @01:54:57.17 Exhaust.select :close @01:54:57.28 SSV.dial CBV @01:54:57.30
CBV.dial SSV @01:54:58.07 Allowing 185s for 25.0ml of remaining 125.0ml @01:54:58.08 Intake.select :open @01:55:00.23 Intake @26.7psia, vacuum from 20.0 to 24.0psi @01:55:00.24 SS.reconfigure forSampling @01:55:00.30 SS.seek 25.00ml @01:55:40.44 Stroke completed -- waiting for pressure to equalize ... @01:55:42.82 Intake.select :close @01:55:42.96 SSV.dial :exhaust @01:55:43.73 Exhaust.select :open @01:55:43.84 SS.reconfigure SSexhale @01:55:43.89 SS.seek 0.00ml @01:55:52.49 Exhaust.select :close @01:55:52.59 SSV.dial CBV @01:55:52.61
CBV.dial SSV @01:55:53.40 Allowing 185s for 25.0ml of remaining 100.0ml @01:55:53.41 Intake.select :open @01:55:55.56 Intake @26.5psia, vacuum from 20.0 to 24.0psi @01:55:55.57 SS.reconfigure forSampling @01:55:55.63 SS.seek 25.00ml @01:56:35.71 Stroke completed -- waiting for pressure to equalize ... @01:56:38.70 Intake.select :close @01:56:38.84 SSV.dial :exhaust @01:56:39.60 Exhaust.select :open @01:56:39.71 SS.reconfigure SSexhale @01:56:39.76 SS.seek 0.00ml @01:56:48.39 Exhaust.select :close @01:56:48.49 SSV.dial CBV @01:56:48.51
CBV.dial SSV @01:56:49.30 Allowing 185s for 25.0ml of remaining 75.0ml @01:56:49.31 Intake.select :open @01:56:51.46 Intake @26.3psia, vacuum from 19.8 to 23.8psi @01:56:51.47 SS.reconfigure forSampling @01:56:51.53 SS.seek 25.00ml @01:57:32.00 Stroke completed -- waiting for pressure to equalize ... @01:57:35.01 Intake.select :close @01:57:35.16 SSV.dial :exhaust @01:57:35.92 Exhaust.select :open @01:57:36.03 SS.reconfigure SSexhale @01:57:36.08 SS.seek 0.00ml @01:57:44.83 Exhaust.select :close @01:57:44.94 SSV.dial CBV @01:57:44.96
CBV.dial SSV @01:57:45.75 Allowing 185s for 25.0ml of remaining 50.0ml @01:57:45.76 Intake.select :open @01:57:47.92 Intake @26.2psia, vacuum from 19.7 to 23.7psi @01:57:47.93 SS.reconfigure forSampling @01:57:47.99 SS.seek 25.00ml @01:58:28.20 Stroke completed -- waiting for pressure to equalize ... @01:58:31.69 Intake.select :close @01:58:31.84 SSV.dial :exhaust @01:58:32.61 Exhaust.select :open @01:58:32.72 SS.reconfigure SSexhale @01:58:32.77 SS.seek 0.00ml @01:58:41.34 Exhaust.select :close @01:58:41.45 SSV.dial CBV @01:58:41.47
CBV.dial SSV @01:58:42.25 Allowing 185s for 25.0ml of remaining 25.0ml @01:58:42.26 Intake.select :open @01:58:44.42 Intake @26.0psia, vacuum from 19.5 to 23.5psi @01:58:44.44 SS.reconfigure forSampling @01:58:44.50 SS.seek 24.99ml @01:59:24.67 Stroke completed -- waiting for pressure to equalize ... @01:59:28.84 Intake.select :close @01:59:28.99 SSV.dial :exhaust @01:59:29.89 Exhaust.select :open @01:59:29.99 SS.reconfigure SSexhale @01:59:30.04 SS.seek 0.00ml @01:59:38.93 Exhaust.select :close @01:59:39.05 Sampled 250.0ml @01:59:39.06 collectSample: Complete @01:59:39.07 Evacuating Puck (bubblePt=55.0psi, delay=15.0s) @01:59:39.09 Intake.select :close @01:59:39.17 CSV.dial :waste @01:59:39.20 CS.seek 0.000ml @01:59:39.32 SSV.dial CBV,avoiding => exhaust @01:59:39.34
CBV.dial SSV @01:59:39.39 CSV.dial :air @01:59:40.34 CS.seek 10.000ml @01:59:41.21
CTV.dial :air @01:59:41.98 delay 15 seconds @01:59:56.98 SSV.dial :spit,avoiding => exhaust @01:59:58.30 CTV.dial CSV,avoiding => intake @01:59:58.32
SS.seek 0.00ml @01:59:58.43 SSV.dial CBV,avoiding => exhaust @02:00:03.89 CBV.dial between CSV and waste2b @02:00:04.85 CTV.dial CSV,avoiding => air @02:00:04.87
CSV.dial CTV @02:00:05.42 CS.reconfigure CSpressurize @02:00:05.47 CS.seek 0.000ml @02:00:31.31 CSV.dial :air @02:00:32.05 CS.reconfigure CSfast @02:00:32.09 CS.seek 10.000ml @02:00:55.16 CSV.dial between air and CTV @02:00:55.48 CS.seek 2.192ml @02:01:13.85 CTV.dial CSV,avoiding => air @02:01:13.86
CSV.dial CTV @02:01:14.18 CS.reconfigure CSpressurize @02:01:14.22 CS.seek 0.000ml @02:01:15.48 BubblePoint: Intake @69.8psia, Outlet @15.0psia @02:01:15.49 SS.reconfigure SSconfig @02:01:15.55 SS.seek 10.00ml @02:01:21.39 delay 15 seconds @02:01:36.49 Evacuated: Intake @69.2psia, Outlet @-0.2psia SSV.dial :spit,avoiding => exhaust @02:01:37.90 SS.reconfigure SSexhale @02:01:37.94 SS.seek 0.00ml @02:01:42.88 SS.reconfigure SSfast @02:01:42.93 SS.seek 5.00ml @02:01:46.18 SSV.dial CBV,avoiding => exhaust @02:01:46.20
CBV.dial SSV @02:01:47.51 CTV.dial :air,avoiding => intake @02:01:48.81 CSV.dial CTV @02:01:48.83
SS.reconfigure SSconfig @02:01:48.89 SS.seek 10.00ml @02:01:49.83 CSV.dial :air @02:01:50.35 CS.reconfigure CSfast @02:01:50.40 CS.seek 0.000ml @02:01:52.03
SSV.dial :spit,avoiding => exhaust @02:01:53.34 SS.reconfigure SSexhale @02:01:53.39 SS.seek 0.00ml @02:01:55.43 CS.coast @02:01:59.03
SS.reconfigure SSfast @02:01:59.08 SS.seek 5.00ml @02:02:01.92 SSV.dial CBV,avoiding => exhaust @02:02:01.94
CBV.dial SSV @02:02:04.24 SSV.dial :spit,avoiding => exhaust @02:02:05.54 SS.reconfigure SSconfig @02:02:05.58 SS.seek 0.00ml @02:02:08.83 SS.coast @02:02:08.87 Evacuating Puck (bubblePt=55.0psi, delay=15.0s) @02:02:08.88 Intake.select :close @02:02:08.96 CSV.dial :waste @02:02:09.06
SSV.dial :exhaust @02:02:09.71 CS.seek 0.000ml @02:02:09.80
Exhaust.select :open @02:02:09.90 SS.reconfigure SSexhale @02:02:09.95 SS.seek 0.00ml @02:02:10.00 CSV.dial :air @02:02:10.41
Exhaust.select :close @02:02:10.52 SSV.dial CBV,avoiding => exhaust @02:02:11.03
CBV.dial SSV @02:02:11.06 CS.seek 10.000ml @02:02:12.90
CTV.dial :air @02:02:12.91 delay 15 seconds @02:02:27.92 SSV.dial :spit,avoiding => exhaust @02:02:29.23 CTV.dial CSV,avoiding => intake @02:02:29.25
SS.seek 0.00ml @02:02:29.35 SSV.dial CBV,avoiding => exhaust @02:02:34.42 CBV.dial between CSV and waste2b @02:02:35.39 CSV.dial between air and CTV @02:02:35.70 CS.seek 7.876ml @02:02:41.31 CTV.dial CSV,avoiding => air @02:02:41.32
CSV.dial CTV @02:02:41.63 CS.reconfigure CSpressurize @02:02:41.68 CS.seek 0.000ml @02:03:02.18 CSV.dial :air @02:03:02.70 CS.reconfigure CSfast @02:03:02.74 CS.seek 10.000ml @02:03:25.81 CSV.dial between air and CTV @02:03:26.42 CS.seek 3.028ml @02:03:42.72 CTV.dial CSV,avoiding => air @02:03:42.73
CSV.dial CTV @02:03:43.14 CS.reconfigure CSpressurize @02:03:43.18 CS.seek 0.000ml @02:03:50.35 BubblePoint: Intake @71.2psia, Outlet @15.9psia @02:03:50.36 SS.reconfigure SSconfig @02:03:50.42 SS.seek 10.00ml @02:03:56.28 delay 15 seconds @02:04:11.38 Evacuated: Intake @69.9psia, Outlet @0.5psia @02:04:11.39 SSV.dial :spit,avoiding => exhaust @02:04:12.71 SS.reconfigure SSexhale @02:04:12.75 SS.seek 0.00ml @02:04:17.70 SS.reconfigure SSfast @02:04:17.75 SS.seek 5.00ml @02:04:20.68 SSV.dial CBV,avoiding => exhaust @02:04:20.70
CBV.dial SSV @02:04:22.02 CTV.dial :air,avoiding => intake @02:04:23.33 CSV.dial CTV @02:04:23.35
SS.reconfigure SSconfig @02:04:23.41 SS.seek 10.00ml @02:04:24.35 CSV.dial :air @02:04:24.89 CS.reconfigure CSfast @02:04:24.94 CS.seek 0.000ml @02:04:26.68
SSV.dial :spit,avoiding => exhaust @02:04:26.77 CS.coast @02:04:27.99
SS.reconfigure SSexhale @02:04:28.04 SS.seek 0.00ml @02:04:33.21 SS.reconfigure SSfast @02:04:33.25 SS.seek 5.00ml @02:04:36.19 SSV.dial CBV,avoiding => exhaust @02:04:36.21
CBV.dial SSV @02:04:38.51 SSV.dial :spit,avoiding => exhaust @02:04:39.83 SS.reconfigure SSconfig @02:04:39.87 SS.seek 0.00ml @02:04:43.11 SS.coast @02:04:43.15 Lysing: Adding 1.5ml lysis @02:04:43.16 CBV.dial between CSV and waste1,avoiding => DBV @02:04:44.65 CTV.dial CSV @02:04:45.93 CSV.dial CSR @02:04:46.73 CSR.select :lysis @02:04:46.84 CS.reconfigure CSconfig @02:04:46.89 CS.seek 1.000ml @02:04:50.21 delay 5 seconds @02:04:55.22 CSR.select :air @02:04:55.38 CS.seek 6.000ml @02:05:08.81 delay 2 seconds @02:05:10.82 CSV.dial :waste1 @02:05:12.10 CS.reconfigure CSfast @02:05:12.14 CS.seek empty @02:05:26.49 CSV.dial CSR @02:05:27.74 CSR.select :lysis @02:05:27.85 CS.reconfigure CSconfig @02:05:27.90 CS.seek 1.500ml @02:05:32.65 delay 5 seconds @02:05:37.66 CSR.select :air @02:05:37.82 CS.seek 4.499ml @02:05:46.21 delay 2 seconds @02:05:48.22 CSV.dial CTV @02:05:48.74 CS.seek 1.275ml @02:05:57.78 Heating sh1 puck from tube 2 in Collection Clamp to 85.0C for 5:00 @02:05:57.79 CH.seek 85.0C,8:00 @02:08:13.63 CH.hold 5:00,85.0C @02:13:13.75 Collection Puck Heater at 85.3C after lysing @02:13:13.76 CS.seek 0.000ml @02:13:17.86 CTV.dial between spare and air,avoiding => intake @02:13:18.86 CSV.dial :air @02:13:19.53 CS.seek 5.000ml @02:13:33.03 CSV.dial CTV @02:13:33.47 delay 2 seconds @02:13:35.48 CSV.dial :air @02:13:36.00 delay 2 seconds @02:13:38.02 CSV.dial CTV @02:13:38.53 delay 2 seconds @02:13:40.55 CSV.dial :air @02:13:41.06 delay 2 seconds @02:13:43.07 CS.seek 0.000ml @02:13:56.53 Recovering Lysate into Collection syringe @02:13:56.54 CSV.dial CBV @02:13:57.55 CBV.dial CSV @02:13:57.91 CS.seek 5.000ml @02:14:11.44 delay 5 seconds @02:14:16.45 CTV.dial :air @02:14:16.94 CS.seek 7.000ml @02:14:22.81 delay 5 seconds @02:14:27.82 CSV.dial :air2 @02:14:28.26 CS.seek 10.000ml @02:14:36.75 CSV.dial between air and CTV,avoiding => CBV @02:14:37.90 Lysing Complete @02:14:37.96 Storing sh1 puck in Collection Clamp to tube 1 @02:14:38.10 SC.reconfigure SCtight @02:14:38.15 SC.seek 1 @02:14:40.44 SC.reconfigure SCloose @02:14:40.62 Elbow.reconfigure TightElbow @02:14:40.67 Elbow.seek 1 @02:14:42.30 Forearm.reconfigure TightForearm @02:14:42.35 Forearm.seek 1 + 800 counts @02:14:43.47 SE.reconfigure SEpick @02:14:43.54 SE.seek beamBreak @02:14:46.90 SE.configure SEconfig @02:14:47.04 Elbow.reconfigure LimpElbow @02:14:47.09 Forearm.reconfigure LooseForearm @02:14:47.14 Forearm.seek 1 @02:14:47.38 Elbow.hold @02:14:47.45 Elbow.reconfigure TightElbow @02:14:47.54 SE.seek 2.04 Pucks below Top Plate @02:14:47.93 Forearm.coast @02:14:47.97 Forearm.seek 1 + 337 counts @02:14:48.27 Forearm.reconfigure TightForearm @02:14:48.32 Forearm.seek 1 + 800 counts @02:14:48.60 SE.seek 3.04 Pucks below Top Plate @02:14:49.13 Forearm.reconfigure LooseForearm @02:14:49.18 Forearm.seek retract @02:14:50.16 Elbow.seek CC @02:14:51.74 Elbow.reconfigure LooseElbow @02:14:51.84 Forearm.seek CC @02:14:52.83 CC.seek sealed @02:14:53.78 Hand.grip @02:14:54.49 CC.seek open @02:14:57.86 Forearm.seek retract @02:14:58.82 Elbow.reconfigure TightElbow @02:14:58.87 Elbow.seek 1 @02:15:00.49 Elbow.reconfigure LimpElbow @02:15:00.55 Forearm.seek 1 @02:15:01.56 Elbow.hold @02:15:01.63 Elbow.reconfigure TightElbow @02:15:01.68 Hand.release @02:15:02.57 Forearm.coast @02:15:02.61 Forearm.seek 1 + 330 counts @02:15:02.86 Forearm.reconfigure TightForearm @02:15:02.91 Forearm.seek 1 + 800 counts @02:15:03.14 SE.seek down @02:15:04.24
Forearm.reconfigure LooseForearm @02:15:04.29 Forearm.seek retract @02:15:05.58 SE.coast @02:15:05.62
SC.coast @02:15:05.66 Tranferring lysate to PRV @02:15:05.67 CSV.dial :air @02:15:05.98 CS.seek 10.000ml @02:15:06.06 CSV.dial between air and CTV @02:15:06.45 AIV.dial :bypass @02:15:06.54 Beginning samplePassC @02:15:06.55 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @02:15:06.72 Elbow.seek FlushPuck::Garage @02:15:08.50 Elbow.reconfigure LooseElbow @02:15:08.59 Forearm.seek garage @02:15:09.65 Hand.grip @02:15:10.45 Forearm.seek retract @02:15:11.51 Elbow.reconfigure TightElbow @02:15:11.56 Elbow.seek CC @02:15:14.38 Elbow.reconfigure LooseElbow @02:15:14.48 Forearm.reconfigure TightForearm @02:15:14.52 Forearm.seek CC @02:15:15.65 CC.seek closed @02:15:19.42 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.95 @02:15:19.49 Hand.release @02:15:20.28 Forearm.reconfigure LooseForearm @02:15:20.33 Forearm.seek retract @02:15:21.29 AWV.select :air @02:15:21.30 AIV.dial :bypass @02:15:21.31 PSV.dial PSR @02:15:22.64 PSR.select :flush @02:15:22.75 PS.seek 1.500ml @02:15:38.75 delay 5 seconds @02:15:43.76 PSV.dial :waste1 @02:15:44.21 PS.seek 0.000ml @02:16:00.75 PSV.dial PSR @02:16:01.20 PSR.select :air @02:16:01.31 PS.seek 2.500ml @02:16:27.57 PRV.dial CBV @02:16:27.59
CBV.dial PRV @02:16:28.37 PSV.dial :mixloop @02:16:29.39 CTV.dial :air,avoiding => intake @02:16:29.40 PTV.dial :PRVmixing @02:16:29.51 PS.seek 0.000ml @02:16:55.75 PSV.dial :air @02:16:56.33 PS.seek 2.500ml @02:17:22.56 PSV.dial :mixloop @02:17:23.02 PS.seek 0.000ml @02:17:49.34 PSV.dial :air @02:17:49.92 PS.seek 2.500ml @02:18:16.21 PSV.dial :mixloop @02:18:16.66 PS.seek 0.000ml @02:18:42.96 Garaging FlushPuck in Collection Clamp @02:18:43.04 Elbow.reconfigure TightElbow @02:18:43.08 Elbow.seek CC @02:18:43.19 Elbow.reconfigure LooseElbow @02:18:43.28 Forearm.seek CC @02:18:44.24 CC.seek sealed @02:18:45.17 Hand.grip @02:18:45.98 CC.seek open @02:18:49.30 Forearm.seek retract @02:18:50.25 Elbow.reconfigure TightElbow @02:18:50.30 Elbow.seek garage @02:18:53.07 Elbow.reconfigure LooseElbow @02:18:53.17 Forearm.reconfigure TightForearm @02:18:53.22 Forearm.seek garage @02:18:54.40 Hand.release @02:18:55.14 Forearm.reconfigure LooseForearm @02:18:55.19 Forearm.seek retract @02:18:56.22 Connecting PS to CS to transfer sample @02:18:56.23 CBV.dial AIV @02:18:56.24 AIV.dial :bypass @02:18:56.27 PSV.dial :mixloop @02:18:56.28 PTV.dial :PRVmixing @02:18:56.30 PRV.dial AIV @02:18:56.31 CTV.dial :air,avoiding => intake @02:18:56.34 Loading lyfil puck from tube 2 into Collection Clamp @02:18:56.54 SC.reconfigure SCtight @02:18:56.59 SC.seek 2 @02:18:58.89 SC.reconfigure SCloose @02:18:59.07 Elbow.reconfigure TightElbow @02:18:59.12 Elbow.seek 2 @02:19:01.09 Forearm.reconfigure TightForearm @02:19:01.14 Forearm.seek 2 + 800 counts @02:19:02.14 SE.reconfigure SEpick @02:19:02.20 SE.seek beamBreak @02:19:04.79 SE.configure SEconfig @02:19:04.92 Elbow.seek 2 @02:19:05.07 Forearm.seek 2 + 300 counts @02:19:05.33 Forearm.reconfigure LooseForearm @02:19:05.38 Hand.grip @02:19:06.10 SE.seek down @02:19:06.26
Forearm.seek retract @02:19:07.24 Elbow.seek CC @02:19:08.49 SE.coast @02:19:08.84
Elbow.reconfigure LooseElbow @02:19:08.93 Forearm.reconfigure TightForearm @02:19:08.97 Forearm.seek CC @02:19:10.00 CC.seek closed @02:19:13.77 Collection Clamp onSpring/offSpring for 4/21 samples, on/off torque 2.04 @02:19:13.78 Hand.release @02:19:14.52 Forearm.reconfigure LooseForearm @02:19:14.56 Forearm.seek retract @02:19:15.51 SC.coast @02:19:15.55 CTV.dial CSV,avoiding => intake @02:19:16.86 CSV.dial CTV @02:19:17.17 CS.seek 5.000ml @02:19:30.70 delay 1:20 @02:20:50.71 CS.seek 2.500ml @02:20:57.86 delay 40 seconds @02:21:37.87 CS.seek 0.000ml @02:21:45.74 delay 1:00 @02:22:45.75 PSV.dial :air @02:22:46.20 PS.seek 2.500ml @02:23:12.46 PSV.dial :mixloop @02:23:12.91 delay 3 seconds @02:23:15.92 PSV.dial :air @02:23:16.38 delay 2 seconds @02:23:18.39 PSV.dial :mixloop @02:23:18.85 delay 3 seconds @02:23:21.87 PSV.dial :waste1 @02:23:23.17 PS.seek empty @02:23:49.43 CBV.dial :waste1 @02:23:49.92 delay 5 seconds @02:23:54.94 CBV.dial AIV @02:23:55.42 delay 10 seconds @02:24:05.44 CBV.dial :waste1 @02:24:05.90 delay 10 seconds @02:24:15.91 CBV.dial AIV @02:24:16.37 delay 10 seconds @02:24:26.38 CTV.dial CSV,avoiding => intake @02:24:26.39 CBV.dial between AIV and waste1 @02:24:26.75 CSV.dial :air @02:24:27.27 CS.seek 10.000ml @02:24:53.45 CSV.dial CTV @02:24:53.97 CS.seek 0.000ml @02:25:20.07 CBV.dial :waste1 @02:25:20.39 delay 10 seconds @02:25:30.40 CTV.dial :air,avoiding => intake @02:25:31.76 Storing lyfil puck in Collection Clamp to tube 1 @02:25:31.90 SC.reconfigure SCtight @02:25:31.94 SC.seek 1 @02:25:34.20 SC.reconfigure SCloose @02:25:34.39 Elbow.reconfigure TightElbow @02:25:34.43 Elbow.seek 1 @02:25:36.06 Forearm.reconfigure TightForearm @02:25:36.11 Forearm.seek 1 + 800 counts @02:25:37.23 SE.reconfigure SEpick @02:25:37.30 SE.seek beamBreak @02:25:40.05 SE.configure SEconfig @02:25:40.18 Elbow.reconfigure LimpElbow @02:25:40.22 Forearm.reconfigure LooseForearm @02:25:40.27 Forearm.seek 1 @02:25:40.54 Elbow.hold @02:25:40.60 Elbow.reconfigure TightElbow @02:25:40.69 SE.seek 3.04 Pucks below Top Plate @02:25:41.01 Forearm.coast @02:25:41.05 Forearm.seek 1 + 339 counts @02:25:41.28 Forearm.reconfigure TightForearm @02:25:41.33 Forearm.seek 1 + 800 counts @02:25:41.59 SE.seek 4.04 Pucks below Top Plate @02:25:42.12 Forearm.reconfigure LooseForearm @02:25:42.17 Forearm.seek retract @02:25:43.12 Elbow.seek CC @02:25:44.68 Elbow.reconfigure LooseElbow @02:25:44.78 Forearm.seek CC @02:25:45.73 CC.seek sealed @02:25:46.67 Hand.grip @02:25:47.47 CC.seek open @02:25:50.83 Forearm.seek retract @02:25:51.79 Elbow.reconfigure TightElbow @02:25:51.84 Elbow.seek 1 @02:25:53.44 Elbow.reconfigure LimpElbow @02:25:53.49 Forearm.seek 1 @02:25:54.52 Elbow.hold @02:25:54.58 Elbow.reconfigure TightElbow @02:25:54.63 Hand.release @02:25:55.42 Forearm.coast @02:25:55.46 Forearm.seek 1 + 335 counts @02:25:55.76 Forearm.reconfigure TightForearm @02:25:55.80 Forearm.seek 1 + 800 counts @02:25:56.02 SE.seek down @02:25:57.13
Forearm.reconfigure LooseForearm @02:25:57.18 Forearm.seek retract @02:25:58.35 SE.coast @02:25:58.40
SC.coast @02:25:58.43 PSV.dial :air @02:25:59.51 PS.seek 0.499ml @02:26:05.52 delay 3 seconds @02:26:08.53 PSV.dial :mixloop @02:26:08.97 PRV.dial :air @02:26:09.42 delay 3 seconds @02:26:12.43 PRV.dial CBV @02:26:12.87 CBV.dial AIV @02:26:13.89 PRV.dial between CBV and air @02:26:14.20 PRV.dial :air @02:26:14.56 PS.reconfigure PSslow @02:26:14.60 PS.seek 0.599ml @02:26:17.14 PRV.dial :waste2 @02:26:17.88 PS.reconfigure PSconfig @02:26:17.92 PS.seek empty @02:26:24.89 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @02:26:25.05 Elbow.seek FlushPuck::Garage @02:26:26.78 Elbow.reconfigure LooseElbow @02:26:26.88 Forearm.seek garage @02:26:27.92 Hand.grip @02:26:28.81 Forearm.seek retract @02:26:29.83 Elbow.reconfigure TightElbow @02:26:29.88 Elbow.seek CC @02:26:32.61 Elbow.reconfigure LooseElbow @02:26:32.70 Forearm.reconfigure TightForearm @02:26:32.74 Forearm.seek CC @02:26:33.85 CC.seek closed @02:26:37.62 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.97 @02:26:37.68 Hand.release @02:26:38.51 Forearm.reconfigure LooseForearm @02:26:38.55 Forearm.seek retract @02:26:39.57 Clearing PML for modified lysate @02:26:39.58 CTV.dial :air,avoiding => intake @02:26:39.60 PRV.dial :waste2 @02:26:39.61 PTV.dial :PRV,avoiding => puck @02:26:40.35 delay 2 seconds @02:26:42.35 PTV.dial :PRVmixing,avoiding => puck @02:26:43.06 delay 2 seconds @02:26:45.08 PSV.dial :PML @02:26:45.09 delay 2 seconds @02:26:47.11 PRV.dial :air @02:26:47.86 delay 2 seconds @02:26:49.92 PS.seek 1.499ml @02:27:05.95 delay 5 seconds @02:27:10.96 PS.seek 2.500ml @02:27:21.92 PSV.dial :air @02:27:22.51 PS.seek 0.000ml @02:27:48.76 mixing lysate with speDil @02:27:48.77 PSV.dial :PML @02:27:49.21 CBV.dial :PRV @02:27:49.22 PRV.dial :air @02:27:49.24 delay 2 seconds @02:27:51.25 PRV.dial :speDil @02:27:51.70 delay 2 seconds @02:27:53.77 PS.reconfigure PSslow1 @02:27:53.81 PS.seek 0.225ml @02:28:17.51 delay 5 seconds @02:28:22.52 PRV.dial CBV @02:28:23.27 delay 2 seconds @02:28:25.34 PS.seek 0.475ml @02:28:51.10 delay 5 seconds @02:28:56.11 PRV.dial :air @02:28:56.55 delay 2 seconds @02:28:58.57 PS.seek 1.000ml @02:29:52.60 delay 10 seconds @02:30:02.61 PRV.dial CBV @02:30:03.08 CTV.dial :air,avoiding => intake @02:30:03.09 PSV.dial :air @02:30:03.56 PS.reconfigure PSconfig @02:30:03.60 PS.seek 2.500ml @02:30:19.63 delay 3 seconds @02:30:22.65 PSV.dial PTV @02:30:23.38 PTV.dial :PRV,avoiding => puck @02:30:24.12 PS.seek 0.000ml @02:30:50.38 PSV.dial :air @02:30:51.25 PS.seek 2.500ml @02:31:17.50 delay 3 seconds @02:31:20.51 PSV.dial PTV @02:31:21.35 PTV.dial :PRV,avoiding => puck @02:31:21.37 PS.seek 0.000ml @02:31:47.56 Pushing modified lysate into AIV Injection Loop @02:31:47.57 CBV.dial AIV @02:31:47.58 AIV.dial :bypass @02:31:47.61 PRV.dial CBV @02:31:47.63 PSV.dial :air @02:31:48.35 PS.seek 2.500ml @02:32:14.56 delay 3 seconds @02:32:17.57 PSV.dial :PML @02:32:18.01 PTV.dial :PRVmixing,avoiding => puck @02:32:18.80 PS.reconfigure PSslow1 @02:32:18.84 PS.seek 1.740ml @02:33:36.43 delay 5 seconds @02:33:41.45 AIV.dial :sample @02:33:42.00 AWV.select :waste @02:33:42.70 PS.reconfigure PSslow2 @02:33:42.75 PS.seek 1.220ml @02:35:28.74 delay 15 seconds @02:35:43.75 AIV.dial :bypass @02:35:44.30 PS.reconfigure PSconfig @02:35:44.34 PS.seek 0.000ml @02:35:57.58 PSV.dial :air @02:35:58.03 PS.seek 2.500ml @02:36:24.24 PSV.dial :PML @02:36:24.70 PS.seek 0.000ml @02:36:51.08 AIV.dial :coilToMFB @02:36:51.09 AWV.select :air @02:36:51.25 Extracting Nucleic Acid from AIV @02:36:51.27 SPE.hold 2:46:40,55.0C @02:36:51.29
Beginning samplePassD @02:36:51.32 ASV.dial :SPEdelivery @02:36:51.45
AWV.select :air @02:36:51.46 AIV.dial :bypass @02:36:51.48 PSV.dial PSR @02:36:52.09 delay 20 seconds @02:36:52.53
PSR.select :killMFB @02:36:52.64 PS.seek 1.500ml @02:37:08.71 delay 5 seconds @02:37:12.10 primeLysateINT @02:37:12.11 AWV.select :air @02:37:12.12 AIV.dial :coilToMFB @02:37:12.14 ATV.dial :AIV @02:37:13.48 AS.reconfigure ASslow1 @02:37:13.53 AS.seek 0.241ml @02:37:13.72
PSV.dial :waste1 @02:37:14.20 PS.seek 0.000ml @02:37:30.27 PSV.dial PSR @02:37:30.75 PSR.select :air @02:37:30.86 PS.seek 2.500ml @02:37:57.11 PRV.dial CBV @02:37:57.13
CBV.dial PRV @02:37:57.15 PSV.dial :mixloop @02:37:58.24 CTV.dial :air,avoiding => intake @02:37:58.25 PTV.dial :PRVmixing @02:37:58.28 PS.seek 0.000ml @02:38:03.19 delay 10 seconds @02:38:13.20 ATV.dial :air2 @02:38:13.79 AS.seek 0.271ml @02:38:20.16 ATV.dial :waste2 @02:38:20.53 AS.reconfigure ASnorm @02:38:20.57 AS.seek empty @02:38:24.58
PSV.dial PSR @02:38:25.66 PSR.select :flush @02:38:25.77 PS.seek 1.500ml @02:38:34.86 Lysate primed! @02:38:34.87 AFV.select :holdingCoil @02:38:34.88 ATV.dial :air2 @02:38:35.30 AS.reconfigure ASslow1 @02:38:35.35 AS.seek 0.069ml @02:38:41.80
delay 5 seconds @02:38:46.82 PSV.dial :waste1 @02:38:47.30 PS.seek 0.000ml @02:38:49.95 delay 3 seconds @02:38:52.96 ATV.dial :AIV @02:38:53.53 AS.seek 0.469ml @02:39:03.40
PSV.dial PSR @02:39:03.88 PSR.select :air @02:39:03.99 PS.seek 2.500ml @02:39:30.21 PRV.dial CBV @02:39:30.23
CBV.dial PRV @02:39:30.25 PSV.dial :mixloop @02:39:31.30 CTV.dial :air,avoiding => intake @02:39:31.31 PTV.dial :PRVmixing @02:39:31.34 PS.seek 0.000ml @02:39:57.58 PSV.dial PSR @02:39:58.62 PSR.select :flush @02:39:58.74 PS.seek 1.500ml @02:40:14.83 delay 5 seconds @02:40:15.39 delay 5 seconds @02:40:19.84
PSV.dial :waste1 @02:40:20.39 PS.seek 0.000ml @02:40:20.48 Solid Phase Extraction Heater at 53.8C @02:40:20.49 ATV.dial ASV @02:40:20.84 ASV.dial :SPEdelivery @02:40:20.92 AS.seek 0.041ml @02:40:36.52
PSV.dial PSR @02:40:36.99 PSR.select :air @02:40:37.10 PS.seek 2.500ml @02:41:03.43 PRV.dial CBV @02:41:03.45
CBV.dial PRV @02:41:03.47 PSV.dial :mixloop @02:41:04.50 CTV.dial :air,avoiding => intake @02:41:04.51 PTV.dial :PRVmixing @02:41:04.54 PS.seek 0.000ml @02:41:30.77 PSV.dial PSR @02:41:31.80 PSR.select :flush @02:41:31.91 PS.seek 1.500ml @02:41:47.92 delay 5 seconds @02:41:48.46 delay 10 seconds @02:41:52.93
PSV.dial :waste1 @02:41:53.39 PS.seek 0.000ml @02:41:58.47 rinseLysateLineINT @02:41:58.48 AFV.select :carrierSolution @02:41:58.58 delay 2 seconds @02:42:00.59 AS.reconfigure ASconfig @02:42:00.64 AS.seek 1.000ml @02:42:09.55
PSV.dial PSR @02:42:10.02 PSR.select :air @02:42:10.13 PS.seek 2.500ml @02:42:36.36 PRV.dial CBV @02:42:36.38
CBV.dial PRV @02:42:36.40 PSV.dial :mixloop @02:42:37.42 CTV.dial :air,avoiding => intake @02:42:37.94 PTV.dial :PRVmixing @02:42:37.96 PS.reconfigure PSslow @02:42:38.01 PS.seek 1.200ml @02:42:56.82 delay 1 second @02:42:57.83 AFV.select :holdingCoil @02:42:57.93 delay 1 second @02:42:58.94 ATV.dial :AIV @02:42:59.27 AIV.dial :coilToMFB @02:42:59.28 AWV.select :waste @02:42:59.39 AS.reconfigure ASslow0 @02:42:59.44 AS.seek 0.000ml @02:43:04.95
PSV.dial :waste1 @02:43:06.29 PS.reconfigure PSconfig @02:43:06.33 PS.seek 0.000ml @02:43:19.36 PSV.dial :air @02:43:20.41 PS.seek 2.500ml @02:43:46.70 PSV.dial :mixloop @02:43:47.16 PS.reconfigure PSslow @02:43:47.21 PS.seek 0.000ml @02:44:38.48 PSV.dial :air @02:44:38.96 PS.reconfigure PSconfig @02:44:39.00 PS.seek 2.500ml @02:44:41.77 ATV.dial :air2 @02:44:42.35 AS.reconfigure ASslow1 @02:44:42.40 AS.seek 0.050ml @02:44:52.92 ATV.dial :AIV @02:44:53.49 AS.seek 0.010ml @02:45:01.89 ATV.dial :waste @02:45:03.17 AS.seek empty @02:45:05.29
PSV.dial :mixloop @02:45:05.64 ethanol wash @02:45:05.65 ATV.dial :air2 @02:45:05.75
PS.reconfigure PSslow @02:45:05.80 PS.seek 0.000ml @02:45:06.60 AS.reconfigure ASslow2 @02:45:06.65 AS.seek 0.009ml @02:45:17.30 delay 3 seconds @02:45:20.31 Pulling 0.4ml of Ethanol Wash @02:45:20.32 ATV.dial :ethanol @02:45:20.91 AS.reconfigure ASslow1 @02:45:20.96 AS.seek 0.409ml @02:45:57.10
Cleaning Collection Syringe @02:45:57.11 CSV.dial CSR @02:45:57.56 CSR.select :flush @02:45:57.67 CS.seek 4.000ml @02:46:08.62 delay 5 seconds @02:46:13.64 CSR.select :air @02:46:13.75 CS.seek 10.000ml @02:46:29.80 CSV.dial CTV @02:46:29.84
CTV.dial CSV @02:46:31.21 CBV.dial :waste1 @02:46:31.70 CS.seek 0.000ml @02:46:42.81 delay 3 seconds @02:46:45.82 ATV.dial :air1 @02:46:46.15 delay 3 seconds @02:46:49.21 AS.seek 0.434ml @02:46:54.61 delay 3 seconds @02:46:57.62 ATV.dial :ethanol @02:46:57.87
CSV.dial CSR @02:46:58.00 AS.seek 0.419ml @02:46:58.30
CSR.select :flush @02:46:58.41 CS.seek 4.000ml @02:47:01.33 delay 3 seconds @02:47:04.34 ATV.dial ASV @02:47:05.45 AS.seek 0.092ml @02:47:09.33
delay 5 seconds @02:47:14.34 CSR.select :air @02:47:14.45 CS.seek 10.000ml @02:47:30.39 CSV.dial CTV @02:47:30.41
CTV.dial CSV @02:47:30.95 CBV.dial :waste1 @02:47:30.96 CS.seek 0.000ml @02:47:57.16 Garaging FlushPuck in Collection Clamp @02:47:57.23 Elbow.reconfigure TightElbow @02:47:57.27 Elbow.seek CC @02:47:57.38 Elbow.reconfigure LooseElbow @02:47:57.47 Forearm.seek CC @02:47:58.46 CC.seek sealed @02:47:59.39 Hand.grip @02:48:00.17 CC.seek open @02:48:03.48 Forearm.seek retract @02:48:04.48 Elbow.reconfigure TightElbow @02:48:04.53 Elbow.seek garage @02:48:07.35 Elbow.reconfigure LooseElbow @02:48:07.44 Forearm.reconfigure TightForearm @02:48:07.48 Forearm.seek garage @02:48:08.69 Hand.release @02:48:09.46 Forearm.reconfigure LooseForearm @02:48:09.51 Forearm.seek retract @02:48:10.56 Whole Cell Archive sampling 1000ml @02:48:10.58 Loading wcr puck from tube 2 into Collection Clamp @02:48:10.78 SC.reconfigure SCtight @02:48:10.83 SC.seek 2 @02:48:12.39 delay 3 seconds @02:48:13.02 SC.reconfigure SCloose @02:48:13.72 Elbow.reconfigure TightElbow @02:48:13.77 Elbow.seek 2 @02:48:15.40 ATV.dial :waste2 @02:48:15.66 Forearm.reconfigure TightForearm @02:48:15.71 Forearm.seek 2 + 800 counts @02:48:15.93 AS.reconfigure ASconfig @02:48:15.98 AS.seek empty @02:48:16.83 SE.reconfigure SEpick @02:48:16.90 SE.seek beamBreak @02:48:19.56 SE.configure SEconfig @02:48:19.71 Elbow.seek 2 @02:48:19.97 Forearm.seek 2 + 300 counts @02:48:20.31 Forearm.reconfigure LooseForearm @02:48:20.36 Hand.grip @02:48:21.06 SE.seek down @02:48:21.20 Forearm.seek retract @02:48:21.68 ATV.dial :air2 @02:48:22.04 AS.reconfigure ASslow1 @02:48:22.09 AS.seek 1.000ml @02:48:22.18 Elbow.seek CC @02:48:23.52 SE.coast @02:48:23.72 Elbow.reconfigure LooseElbow @02:48:23.82 Forearm.reconfigure TightForearm @02:48:23.87 Forearm.seek CC @02:48:25.02 CC.seek closed @02:48:28.79 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.97 @02:48:28.80 Hand.release @02:48:29.58 Forearm.reconfigure LooseForearm @02:48:29.63 Forearm.seek retract @02:48:30.62 SC.coast @02:48:30.67 collectSample 1000ml w/bubble point of 55psi @02:48:30.68 Priming 7ml @02:48:30.69 Intake.select :close @02:48:30.70 Exhaust.select :close @02:48:30.71 SS.hold @02:48:30.90 Calibrating pressure sensors @02:48:30.91 CTV.dial :air @02:48:32.21 SSV.dial :spit,avoiding => exhaust @02:48:32.22 SS.reconfigure SSfast @02:48:32.28 SS.seek 5.00ml @02:48:35.20 SSV.dial CBV @02:48:35.22 CBV.dial SSV @02:48:36.60 delay 3.8 seconds @02:48:40.50 SSV.dial :spit @02:48:41.83 SS.seek 0.00ml @02:48:44.42 SSV.dial CBV @02:48:45.76 CTV.dial :intake @02:48:46.51 SSV.dial CBV @02:48:46.52 CBV.dial SSV @02:48:46.54 Allowing 55s for 7.0ml of remaining 7.0ml @02:48:46.56 Intake.select :open @02:48:48.72 Intake @25.8psia, vacuum from 19.3 to 23.3psi @02:48:48.74 SS.reconfigure forSampling @02:48:48.80 SS.seek 7.00ml @02:48:54.21 Stroke completed -- waiting for pressure to equalize ... @02:49:29.58 Intake.select :close @02:49:29.73 SSV.dial :exhaust @02:49:30.50 Exhaust.select :open @02:49:30.60 SS.reconfigure SSexhale @02:49:30.65 SS.seek 0.00ml @02:49:34.36 Exhaust.select :close @02:49:34.47 Sampling 1000.0ml Intake.select :close @02:49:34.48 Exhaust.select :close @02:49:34.51 SS.reconfigure SSconfig @02:49:34.56 SS.hold @02:49:34.75 CTV.dial :intake @02:49:34.76 SSV.dial CBV @02:49:34.79 CBV.dial SSV @02:49:35.58 Allowing 185s for 25.0ml of remaining 1000.0ml @02:49:35.59 Intake.select :open @02:49:37.74 Intake @25.8psia, vacuum from 19.3 to 23.3psi @02:49:37.76 SS.reconfigure forSampling @02:49:37.82 SS.seek 25.00ml @02:49:41.25 Vacuum too high -- waiting up to 40s @02:49:43.27 Intake @24.0psia, vacuum from 17.5 to 21.5psi @02:49:43.28 SS.reconfigure forSampling @02:49:43.33 SS.seek 25.00ml @02:49:45.54 Vacuum too high -- waiting up to 40s @02:49:47.96 Intake @24.4psia, vacuum from 17.9 to 21.9psi @02:49:47.98 SS.reconfigure forSampling @02:49:48.04 SS.seek 25.00ml @02:49:50.73 Vacuum too high -- waiting up to 40s @02:49:53.14 Intake @24.2psia, vacuum from 17.7 to 21.7psi @02:49:53.15 SS.reconfigure forSampling @02:49:53.20 SS.seek 25.00ml @02:49:55.98 Vacuum too high -- waiting up to 40s @02:49:58.68 Intake @24.3psia, vacuum from 17.8 to 21.8psi @02:49:58.69 SS.reconfigure forSampling @02:49:58.75 SS.seek 25.00ml @02:50:02.69 Vacuum too high -- waiting up to 40s @02:50:05.83 SS.reconfigure forSampling @02:50:05.88 SS.seek 25.00ml @02:50:12.10 Vacuum too high -- waiting up to 40s @02:50:15.86 Intake @24.2psia, vacuum from 17.7 to 21.7psi @02:50:15.87 SS.reconfigure forSampling @02:50:15.92 SS.seek 25.00ml @02:50:18.03 Stroke completed -- waiting for pressure to equalize ... @02:50:22.19 Intake.select :close @02:50:22.34 SSV.dial :exhaust @02:50:23.11 Exhaust.select :open @02:50:23.21 SS.reconfigure SSexhale @02:50:23.26 SS.seek 0.00ml @02:50:32.36 Exhaust.select :close @02:50:32.46 SSV.dial CBV @02:50:32.48 CBV.dial SSV @02:50:33.25 Allowing 185s for 25.0ml of remaining 975.0ml @02:50:33.27 Intake.select :open @02:50:35.42 Intake @25.6psia, vacuum from 19.1 to 23.1psi @02:50:35.44 SS.reconfigure forSampling @02:50:35.50 SS.seek 25.00ml @02:50:41.65 Vacuum too high -- waiting up to 40s @02:50:42.98 Intake @24.0psia, vacuum from 17.5 to 21.5psi @02:50:42.99 SS.reconfigure forSampling @02:50:43.04 SS.seek 25.00ml @02:51:15.54 Stroke completed -- waiting for pressure to equalize ... @02:51:22.02 Intake.select :close @02:51:22.16 SSV.dial :exhaust @02:51:22.93 Exhaust.select :open @02:51:23.03 SS.reconfigure SSexhale @02:51:23.08 SS.seek 0.00ml @02:51:31.79 Exhaust.select :close @02:51:31.89 SSV.dial CBV @02:51:31.91 CBV.dial SSV @02:51:32.70 Allowing 185s for 25.0ml of remaining 950.0ml @02:51:32.72 Intake.select :open @02:51:34.87 Intake @25.4psia, vacuum from 18.9 to 22.9psi @02:51:34.89 SS.reconfigure forSampling @02:51:34.95 SS.seek 25.00ml @02:51:46.66 delay 5 seconds @02:51:51.67 ATV.dial ASV @02:51:52.38 ASV.dial :SPEdelivery @02:51:52.40 AS.seek 0.020ml @02:52:15.10 Stroke completed -- waiting for pressure to equalize ... @02:52:22.82 Intake.select :close @02:52:22.97 SSV.dial :exhaust @02:52:23.74 Exhaust.select :open @02:52:23.84 SS.reconfigure SSexhale @02:52:23.89 SS.seek 0.00ml @02:52:32.79 Exhaust.select :close @02:52:32.89 SSV.dial CBV @02:52:32.91 CBV.dial SSV @02:52:33.70 Allowing 185s for 25.0ml of remaining 925.0ml @02:52:33.72 Intake.select :open @02:52:35.87 Intake @25.3psia, vacuum from 18.8 to 22.8psi @02:52:35.89 SS.reconfigure forSampling @02:52:35.95 SS.seek 25.00ml @02:53:16.13 Stroke completed -- waiting for pressure to equalize ... @02:53:25.05 Intake.select :close @02:53:25.20 SSV.dial :exhaust @02:53:25.99 Exhaust.select :open @02:53:26.09 SS.reconfigure SSexhale @02:53:26.14 SS.seek 0.00ml @02:53:34.80 Exhaust.select :close @02:53:34.90 SSV.dial CBV @02:53:34.92 CBV.dial SSV @02:53:35.69 Allowing 185s for 25.0ml of remaining 900.0ml @02:53:35.71 Intake.select :open @02:53:37.86 Intake @25.3psia, vacuum from 18.8 to 22.8psi @02:53:37.88 SS.reconfigure forSampling @02:53:37.94 SS.seek 25.00ml @02:54:10.67 Vacuum too high -- waiting up to 40s @02:54:13.96 Intake @24.0psia, vacuum from 17.5 to 21.5psi @02:54:13.98 SS.reconfigure forSampling @02:54:14.04 SS.seek 25.00ml @02:54:25.22 Stroke completed -- waiting for pressure to equalize ... @02:54:29.38 Intake.select :close @02:54:29.52 SSV.dial :exhaust @02:54:30.28 Exhaust.select :open @02:54:30.39 SS.reconfigure SSexhale @02:54:30.44 SS.seek 0.00ml @02:54:39.16 Exhaust.select :close @02:54:39.26 SSV.dial CBV @02:54:39.28 CBV.dial SSV @02:54:40.67 Allowing 185s for 25.0ml of remaining 875.0ml @02:54:40.68 Intake.select :open @02:54:42.82 Intake @25.3psia, vacuum from 18.8 to 22.8psi @02:54:42.84 SS.reconfigure forSampling @02:54:42.90 SS.seek 25.00ml @02:55:12.54 delay 5 seconds @02:55:17.55 ATV.dial :air2 @02:55:18.31 AS.seek 0.520ml @02:55:43.11 Stroke completed -- waiting for pressure to equalize ... @02:55:46.70 Intake.select :close @02:55:46.84 SSV.dial :exhaust @02:55:47.62 Exhaust.select :open @02:55:47.72 SS.reconfigure SSexhale @02:55:47.77 SS.seek 0.00ml @02:55:56.54 Exhaust.select :close @02:55:56.64 SSV.dial CBV @02:55:56.66 CBV.dial SSV @02:55:57.43 Allowing 185s for 25.0ml of remaining 850.0ml @02:55:57.45 Intake.select :open @02:55:59.60 Intake @25.2psia, vacuum from 18.7 to 22.7psi @02:55:59.62 SS.reconfigure forSampling @02:55:59.68 SS.seek 25.00ml @02:56:59.86 Stroke completed -- waiting for pressure to equalize ... @02:57:00.60 delay 5 seconds @02:57:04.04 Intake.select :close @02:57:04.19 SSV.dial :exhaust @02:57:04.96 Exhaust.select :open @02:57:05.07 SS.reconfigure SSexhale @02:57:05.12 SS.seek 0.00ml @02:57:05.61 ATV.dial ASV @02:57:06.31 ASV.dial :wasteR @02:57:06.81 AS.seek 0.300ml @02:57:13.89 Exhaust.select :close @02:57:13.99 SSV.dial CBV @02:57:14.01 CBV.dial SSV @02:57:14.80 Allowing 185s for 25.0ml of remaining 825.0ml @02:57:14.82 Intake.select :open @02:57:16.97 Intake @25.0psia, vacuum from 18.5 to 22.5psi @02:57:16.99 SS.reconfigure forSampling @02:57:17.05 SS.seek 25.00ml @02:57:51.94 delay 20 seconds @02:58:11.95 AS.seek 0.070ml @02:58:16.84 Stroke completed -- waiting for pressure to equalize ... @02:58:22.17 Intake.select :close @02:58:22.32 SSV.dial :exhaust @02:58:23.09 Exhaust.select :open @02:58:23.19 SS.reconfigure SSexhale @02:58:23.24 SS.seek 0.00ml @02:58:32.08 Exhaust.select :close @02:58:32.18 SSV.dial CBV @02:58:32.20 CBV.dial SSV @02:58:32.98 Allowing 185s for 25.0ml of remaining 800.0ml @02:58:33.00 Intake.select :open @02:58:35.15 Intake @25.0psia, vacuum from 18.5 to 22.5psi @02:58:35.17 SS.reconfigure forSampling @02:58:35.23 SS.seek 25.00ml @02:58:59.12 delay 20 seconds @02:59:19.13 ATV.dial :waste2 @02:59:19.66 AS.reconfigure ASconfig @02:59:19.70 AS.seek empty @02:59:24.07 Elution @02:59:24.08 ATV.dial :air1 @02:59:25.03 AS.reconfigure ASslow1 @02:59:25.08 AS.seek 0.049ml @02:59:35.31 Stroke completed -- waiting for pressure to equalize ... @02:59:35.59 delay 3 seconds @02:59:38.60 ATV.dial :ElutionWater @02:59:39.17 AS.seek 0.109ml @02:59:40.63 Intake.select :close @02:59:40.78 SSV.dial :exhaust @02:59:41.54 Exhaust.select :open @02:59:41.65 SS.reconfigure SSexhale @02:59:41.70 SS.seek 0.00ml @02:59:50.45 Exhaust.select :close @02:59:50.55 SSV.dial CBV @02:59:51.14 CBV.dial SSV @02:59:51.67 delay 3 seconds @02:59:51.94 Allowing 185s for 25.0ml of remaining 775.0ml @02:59:51.95 Intake.select :open @02:59:54.10 Intake @24.9psia, vacuum from 18.4 to 22.4psi @02:59:54.12 SS.reconfigure forSampling @02:59:54.18 SS.seek 25.00ml @02:59:54.68 ATV.dial ASV,via => air2 @02:59:56.42 ASV.dial :SPEelution @02:59:57.11 AFV.select :carrierSolution @02:59:57.27 AS.reconfigure ASnorm @02:59:57.31 AS.seek 0.759ml @03:00:30.79 delay 3 seconds @03:00:33.80 AFV.select :holdingCoil @03:00:33.95 AS.reconfigure ASslow1 @03:00:33.99 AS.seek 0.489ml @03:00:53.99 Stroke completed -- waiting for pressure to equalize ... @03:01:00.52 Intake.select :close @03:01:00.67 SSV.dial :exhaust @03:01:01.44 Exhaust.select :open @03:01:01.54 SS.reconfigure SSexhale @03:01:01.59 SS.seek 0.00ml @03:01:10.48 Exhaust.select :close @03:01:10.58 SSV.dial CBV @03:01:10.60 CBV.dial SSV @03:01:11.38 Allowing 185s for 25.0ml of remaining 750.0ml @03:01:11.40 Intake.select :open @03:01:13.55 Intake @25.0psia, vacuum from 18.5 to 22.5psi @03:01:13.57 SS.reconfigure forSampling @03:01:13.63 SS.seek 25.00ml @03:01:29.32 delay 5 seconds @03:01:34.38 AS.reconfigure ASslow2 @03:01:34.43 AS.seek 0.454ml @03:02:10.49 delay 1:00 @03:02:13.33 Stroke completed -- waiting for pressure to equalize ... @03:02:20.43 Intake.select :close @03:02:20.64 SSV.dial :exhaust @03:02:21.42 Exhaust.select :open @03:02:21.52 SS.reconfigure SSexhale @03:02:21.57 SS.seek 0.00ml @03:02:30.44 Exhaust.select :close @03:02:30.54 SSV.dial CBV @03:02:30.56 CBV.dial SSV @03:02:31.33 Allowing 185s for 25.0ml of remaining 725.0ml @03:02:31.35 Intake.select :open @03:02:33.50 Intake @24.9psia, vacuum from 18.4 to 22.4psi @03:02:33.52 SS.reconfigure forSampling @03:02:33.58 SS.seek 25.00ml @03:03:10.50 pushing eluate to holding coil @03:03:10.56 AS.seek 0.294ml @03:03:33.34 Stroke completed -- waiting for pressure to equalize ... @03:03:40.40 Intake.select :close @03:03:40.54 SSV.dial :exhaust @03:03:41.31 Exhaust.select :open @03:03:41.42 SS.reconfigure SSexhale @03:03:41.47 SS.seek 0.00ml @03:03:50.37 Exhaust.select :close @03:03:50.47 SSV.dial CBV @03:03:50.49 CBV.dial SSV @03:03:51.25 Allowing 185s for 25.0ml of remaining 700.0ml @03:03:51.27 Intake.select :open @03:03:53.41 Intake @24.8psia, vacuum from 18.3 to 22.3psi @03:03:53.43 SS.reconfigure forSampling @03:03:53.48 SS.seek 25.00ml @03:04:53.73 Stroke completed -- waiting for pressure to equalize ... @03:05:01.51 Intake.select :close @03:05:01.65 SSV.dial :exhaust @03:05:02.42 Exhaust.select :open @03:05:02.53 SS.reconfigure SSexhale @03:05:02.58 SS.seek 0.00ml @03:05:11.42 Exhaust.select :close @03:05:11.52 SSV.dial CBV @03:05:11.54 CBV.dial SSV @03:05:12.31 Allowing 185s for 25.0ml of remaining 675.0ml @03:05:12.33 Intake.select :open @03:05:14.49 Intake @24.8psia, vacuum from 18.3 to 22.3psi @03:05:14.50 SS.reconfigure forSampling @03:05:14.55 SS.seek 25.00ml @03:05:54.03 delay 30 seconds @03:06:14.48 Stroke completed -- waiting for pressure to equalize ... @03:06:22.77 Intake.select :close @03:06:22.92 SSV.dial :exhaust @03:06:23.69 Exhaust.select :open @03:06:23.80 SS.reconfigure SSexhale @03:06:23.85 SS.seek 0.00ml @03:06:24.04 ATV.dial :waste,via => air3 @03:06:25.49 AS.reconfigure ASconfig @03:06:25.53 AS.seek empty @03:06:32.63 Exhaust.select :close @03:06:32.73 SSV.dial CBV @03:06:32.75 CBV.dial SSV @03:06:33.54 Allowing 185s for 25.0ml of remaining 650.0ml @03:06:33.56 Intake.select :open @03:06:35.71 Intake @24.8psia, vacuum from 18.3 to 22.3psi @03:06:35.73 SS.reconfigure forSampling @03:06:35.79 SS.seek 25.00ml @03:06:43.01 Parking Bleach in LysateSource line and SPEcolumn @03:06:43.02 ATV.dial :air3 @03:06:43.69 AS.reconfigure ASslow1 @03:06:43.74 AS.seek 0.029ml @03:06:50.18 ATV.dial :ElutionWater @03:06:50.56 AS.seek 0.059ml @03:06:56.97 ATV.dial :air3 @03:06:57.36 AS.seek 0.069ml @03:06:59.67 delay 2 seconds @03:07:01.68 ATV.dial :bleach @03:07:02.16 AS.seek 0.711ml @03:07:35.64 Stroke completed -- waiting for pressure to equalize ... @03:07:45.07 Intake.select :close @03:07:45.23 SSV.dial :exhaust @03:07:46.00 Exhaust.select :open @03:07:46.10 SS.reconfigure SSexhale @03:07:46.15 SS.seek 0.00ml @03:07:55.02 Exhaust.select :close @03:07:55.12 SSV.dial CBV @03:07:55.14 CBV.dial SSV @03:07:55.92 Allowing 185s for 25.0ml of remaining 625.0ml @03:07:55.94 Intake.select :open @03:07:58.08 Intake @24.6psia, vacuum from 18.1 to 22.1psi @03:07:58.10 SS.reconfigure forSampling @03:07:58.15 SS.seek 25.00ml @03:08:58.17 Stroke completed -- waiting for pressure to equalize ... @03:09:08.30 Intake.select :close @03:09:08.50 SSV.dial :exhaust @03:09:09.26 Exhaust.select :open @03:09:09.37 SS.reconfigure SSexhale @03:09:09.42 SS.seek 0.00ml @03:09:13.37 delay 3 seconds @03:09:16.38 ATV.dial :air3 @03:09:16.79 delay 3 seconds @03:09:18.21 Exhaust.select :close @03:09:18.31 SSV.dial CBV @03:09:18.33 CBV.dial SSV @03:09:19.13 Allowing 185s for 25.0ml of remaining 600.0ml @03:09:19.14 Intake.select :open @03:09:19.85 AS.seek 0.736ml @03:09:21.30 Intake @24.6psia, vacuum from 18.1 to 22.1psi @03:09:21.31 SS.reconfigure forSampling @03:09:21.37 SS.seek 25.00ml @03:09:25.21 delay 3 seconds @03:09:28.22 ATV.dial :bleach @03:09:28.60 AS.seek 0.721ml @03:09:31.91 delay 3 seconds @03:09:34.92 ATV.dial :AIV @03:09:36.27 AS.reconfigure ASconfig @03:09:36.31 AS.seek 0.015ml @03:10:17.76 ATV.dial :waste @03:10:19.14 AS.seek empty @03:10:20.35 ATV.dial :air3 @03:10:20.93 AS.reconfigure ASslow1 @03:10:20.98 AS.seek 0.009ml @03:10:21.35 Stroke completed -- waiting for pressure to equalize ... @03:10:23.35 delay 2 seconds @03:10:25.36 ATV.dial :bleach @03:10:25.74 AS.seek 0.579ml @03:10:31.40 Intake.select :close @03:10:31.55 SSV.dial :exhaust @03:10:32.32 Exhaust.select :open @03:10:32.42 SS.reconfigure SSexhale @03:10:32.47 SS.seek 0.00ml @03:10:41.35 Exhaust.select :close @03:10:41.45 SSV.dial CBV @03:10:41.47 CBV.dial SSV @03:10:42.24 Allowing 185s for 25.0ml of remaining 575.0ml @03:10:42.26 Intake.select :open @03:10:44.41 Intake @24.4psia, vacuum from 17.9 to 21.9psi @03:10:44.43 SS.reconfigure forSampling @03:10:44.49 SS.seek 25.00ml @03:11:44.47 Stroke completed -- waiting for pressure to equalize ... @03:11:56.22 Intake.select :close @03:11:56.36 SSV.dial :exhaust @03:11:57.13 Exhaust.select :open @03:11:57.24 SS.reconfigure SSexhale @03:11:57.29 SS.seek 0.00ml @03:12:06.27 Exhaust.select :close @03:12:06.37 SSV.dial CBV @03:12:06.39 CBV.dial SSV @03:12:07.17 Allowing 185s for 25.0ml of remaining 550.0ml @03:12:07.19 Intake.select :open @03:12:09.95 Intake @24.4psia, vacuum from 17.9 to 21.9psi @03:12:09.96 SS.reconfigure forSampling @03:12:10.02 SS.seek 25.00ml @03:12:22.27 delay 3 seconds @03:12:25.28 ATV.dial :air3 @03:12:25.64 delay 3 seconds @03:12:28.69 AS.seek 0.604ml @03:12:34.05 delay 3 seconds @03:12:37.06 ATV.dial :bleach @03:12:37.46 AS.seek 0.589ml @03:12:40.78 delay 3 seconds @03:12:43.79 ATV.dial :ASV @03:12:44.77 ASV.dial :wasteR @03:12:45.45 AS.seek 0.000ml @03:13:10.01 Stroke completed -- waiting for pressure to equalize ... @03:13:22.45 Intake.select :close @03:13:22.60 SSV.dial :exhaust @03:13:23.37 Exhaust.select :open @03:13:23.47 SS.reconfigure SSexhale @03:13:23.52 SS.seek 0.00ml @03:13:32.37 Exhaust.select :close @03:13:32.47 SSV.dial CBV @03:13:32.49 CBV.dial SSV @03:13:33.26 Allowing 185s for 25.0ml of remaining 525.0ml @03:13:33.28 Intake.select :open @03:13:35.43 Intake @24.3psia, vacuum from 17.8 to 21.8psi @03:13:35.45 SS.reconfigure forSampling @03:13:35.51 SS.seek 25.00ml @03:14:35.66 Stroke completed -- waiting for pressure to equalize ... @03:14:45.90 delay 1:00 @03:14:48.66 Intake.select :close @03:14:48.80 SSV.dial :exhaust @03:14:49.57 Exhaust.select :open @03:14:49.67 SS.reconfigure SSexhale @03:14:49.72 SS.seek 0.00ml @03:14:58.63 Exhaust.select :close @03:14:58.73 SSV.dial CBV @03:14:58.75 CBV.dial SSV @03:14:59.53 Allowing 185s for 25.0ml of remaining 500.0ml @03:14:59.55 Intake.select :open @03:15:01.70 Intake @24.2psia, vacuum from 17.7 to 21.7psi @03:15:01.72 SS.reconfigure forSampling @03:15:01.78 SS.seek 25.00ml @03:15:45.91 AFV.select :carrierSolution @03:15:46.01 delay 2 seconds @03:15:48.02 AS.reconfigure ASconfig @03:15:48.07 AS.seek 1.000ml @03:16:01.75 Stroke completed -- waiting for pressure to equalize ... @03:16:15.94 Intake.select :close @03:16:16.09 SSV.dial :exhaust @03:16:16.86 Exhaust.select :open @03:16:16.97 SS.reconfigure SSexhale @03:16:17.02 SS.seek 0.00ml @03:16:25.86 Exhaust.select :close @03:16:25.96 SSV.dial CBV @03:16:25.98 CBV.dial SSV @03:16:26.76 Allowing 185s for 25.0ml of remaining 475.0ml @03:16:26.78 Intake.select :open @03:16:28.93 Intake @24.1psia, vacuum from 17.6 to 21.6psi @03:16:28.95 SS.reconfigure forSampling @03:16:29.01 SS.seek 25.00ml @03:16:46.71 delay 1 second @03:16:47.72 AFV.select :holdingCoil @03:16:47.82 delay 1 second @03:16:48.83 ATV.dial :ASV @03:16:48.84 ASV.dial :wasteR @03:16:48.85 AS.reconfigure ASslow1 @03:16:48.90 AS.seek 0.000ml @03:17:29.07 Stroke completed -- waiting for pressure to equalize ... @03:17:44.39 Intake.select :close @03:17:44.54 SSV.dial :exhaust @03:17:45.31 Exhaust.select :open @03:17:45.41 SS.reconfigure SSexhale @03:17:45.46 SS.seek 0.00ml @03:17:54.46 Exhaust.select :close @03:17:54.56 SSV.dial CBV @03:17:54.58 CBV.dial SSV @03:17:55.37 Allowing 185s for 25.0ml of remaining 450.0ml @03:17:55.39 Intake.select :open @03:17:57.54 Intake @24.0psia, vacuum from 17.5 to 21.5psi @03:17:57.56 SS.reconfigure forSampling @03:17:57.62 SS.seek 25.00ml @03:18:35.02 Vacuum too high -- waiting up to 40s @03:18:39.61 Intake @23.3psia, vacuum from 16.8 to 20.8psi @03:18:39.62 SS.reconfigure forSampling @03:18:39.68 SS.seek 25.00ml @03:19:21.47 Stroke completed -- waiting for pressure to equalize ... @03:19:26.88 Intake.select :close @03:19:27.02 SSV.dial :exhaust @03:19:27.79 Exhaust.select :open @03:19:27.90 SS.reconfigure SSexhale @03:19:27.95 SS.seek 0.00ml @03:19:36.79 Exhaust.select :close @03:19:36.90 SSV.dial CBV @03:19:36.92 CBV.dial SSV @03:19:37.71 Allowing 185s for 25.0ml of remaining 425.0ml @03:19:37.73 Intake.select :open @03:19:39.98 Intake @23.7psia, vacuum from 17.2 to 21.2psi @03:19:40.00 SS.reconfigure forSampling @03:19:40.06 SS.seek 25.00ml @03:20:13.17 delay 20 seconds @03:20:33.18 ATV.dial :air3 @03:20:34.28 AS.seek 0.590ml @03:21:30.50 Stroke completed -- waiting for pressure to equalize ... @03:21:35.86 Intake.select :close @03:21:36.00 SSV.dial :exhaust @03:21:36.76 Exhaust.select :open @03:21:36.87 SS.reconfigure SSexhale @03:21:36.97 SS.seek 0.00ml @03:21:45.75 Exhaust.select :close @03:21:45.85 SSV.dial CBV @03:21:45.87 CBV.dial SSV @03:21:46.65 Allowing 185s for 25.0ml of remaining 400.0ml @03:21:46.67 Intake.select :open @03:21:48.82 Intake @23.7psia, vacuum from 17.2 to 21.2psi @03:21:48.84 SS.reconfigure forSampling @03:21:48.90 SS.seek 25.00ml @03:22:34.90 delay 3 seconds @03:22:37.91 ATV.dial :ASV @03:22:39.07 ASV.dial :wasteR @03:22:39.14 AS.seek 0.090ml @03:23:39.35 Stroke completed -- waiting for pressure to equalize ... @03:23:45.34 Intake.select :close @03:23:45.49 SSV.dial :exhaust @03:23:46.26 Exhaust.select :open @03:23:46.36 SS.reconfigure SSexhale @03:23:46.41 SS.seek 0.00ml @03:23:55.24 Exhaust.select :close @03:23:55.34 SSV.dial CBV @03:23:55.36 CBV.dial SSV @03:23:56.15 Allowing 185s for 25.0ml of remaining 375.0ml @03:23:56.17 Intake.select :open @03:23:58.32 Intake @23.5psia, vacuum from 17.0 to 21.0psi @03:23:58.34 SS.reconfigure forSampling @03:23:58.40 SS.seek 25.00ml @03:24:21.38 delay 30 seconds @03:24:51.39 ATV.dial :extSrc @03:24:51.89 delay 3 seconds @03:24:54.90 ATV.dial :ElutionWater @03:24:55.82 AS.seek 0.120ml @03:25:02.22 ATV.dial :air3 @03:25:02.61 AS.seek 0.130ml @03:25:04.83 delay 2 seconds @03:25:06.84 ATV.dial :bleach @03:25:07.21 AS.seek 0.230ml @03:25:27.86 delay 3 seconds @03:25:30.87 ATV.dial :air3 @03:25:31.20 delay 3 seconds @03:25:34.26 AS.seek 0.255ml @03:25:39.64 delay 3 seconds @03:25:42.65 ATV.dial :bleach @03:25:43.03 AS.seek 0.240ml @03:25:46.34 delay 3 seconds @03:25:48.83 Stroke completed -- waiting for pressure to equalize ... @03:25:49.35 ATV.dial :ASV @03:25:50.27 ASV.dial :SPEdelivery @03:25:50.75 AFV.select :carrierSolution @03:25:50.85 delay 2 seconds @03:25:52.86 AS.reconfigure ASconfig @03:25:52.92 AS.seek 0.500ml @03:25:55.45 Intake.select :close @03:25:55.60 SSV.dial :exhaust @03:25:56.94 Exhaust.select :open @03:25:57.05 SS.reconfigure SSexhale @03:25:57.10 SS.seek 0.00ml @03:26:06.25 Exhaust.select :close @03:26:06.35 SSV.dial CBV @03:26:06.37 CBV.dial SSV @03:26:07.15 Allowing 185s for 25.0ml of remaining 350.0ml @03:26:07.17 Intake.select :open @03:26:08.45 delay 1 second @03:26:09.32 Intake @23.3psia, vacuum from 16.8 to 20.8psi @03:26:09.34 SS.reconfigure forSampling @03:26:09.40 SS.seek 25.00ml @03:26:09.46 AFV.select :holdingCoil @03:26:09.61 AS.reconfigure ASslow1 @03:26:09.66 AS.seek 0.170ml @03:27:17.23 SPE.hold 2:46:40,95.0C @03:27:17.25 delay 3:00 @03:27:59.73 Stroke completed -- waiting for pressure to equalize ... @03:28:06.27 Intake.select :close @03:28:06.41 SSV.dial :exhaust @03:28:07.21 Exhaust.select :open @03:28:07.32 SS.reconfigure SSexhale @03:28:07.37 SS.seek 0.00ml @03:28:16.26 Exhaust.select :close @03:28:16.36 SSV.dial CBV @03:28:16.38 CBV.dial SSV @03:28:17.19 Allowing 185s for 25.0ml of remaining 325.0ml @03:28:17.20 Intake.select :open @03:28:19.35 Intake @23.3psia, vacuum from 16.8 to 20.8psi @03:28:19.37 SS.reconfigure forSampling @03:28:19.43 SS.seek 25.00ml @03:30:10.37 Stroke completed -- waiting for pressure to equalize ... @03:30:16.27 Intake.select :close @03:30:16.43 SSV.dial :exhaust @03:30:17.25 Exhaust.select :open @03:30:17.30 AFV.select :carrierSolution @03:30:17.36 SS.reconfigure SSexhale @03:30:17.41 SS.seek 0.00ml @03:30:17.46 delay 2 seconds @03:30:19.47 AS.reconfigure ASconfig @03:30:19.52 AS.seek 1.000ml @03:30:26.28 Exhaust.select :close @03:30:26.38 SSV.dial CBV @03:30:26.40 CBV.dial SSV @03:30:27.20 Allowing 185s for 25.0ml of remaining 300.0ml @03:30:27.22 Intake.select :open @03:30:29.37 Intake @23.2psia, vacuum from 16.7 to 20.7psi @03:30:29.39 SS.reconfigure forSampling @03:30:29.45 SS.seek 25.00ml @03:31:08.20 delay 1 second @03:31:09.21 AFV.select :holdingCoil @03:31:09.31 delay 1 second @03:31:10.32 ASV.dial :wasteD @03:31:11.63 AS.seek 0.000ml @03:32:10.26 Priming Eluate @03:32:10.27 AFV.select :carrierSolution @03:32:10.38 delay 2 seconds @03:32:12.39 AS.seek 0.500ml @03:32:19.89 Stroke completed -- waiting for pressure to equalize ... @03:32:26.96 Intake.select :close @03:32:27.10 SSV.dial :exhaust @03:32:27.86 Exhaust.select :open @03:32:27.96 SS.reconfigure SSexhale @03:32:28.01 SS.seek 0.00ml @03:32:36.96 Exhaust.select :close @03:32:37.06 SSV.dial CBV @03:32:37.08 CBV.dial SSV @03:32:37.86 Allowing 185s for 25.0ml of remaining 275.0ml @03:32:37.88 Intake.select :open @03:32:40.03 Intake @23.1psia, vacuum from 16.6 to 20.6psi @03:32:40.05 SS.reconfigure forSampling @03:32:40.11 SS.seek 25.00ml @03:32:41.84 delay 1 second @03:32:42.85 AFV.select :holdingCoil @03:32:42.95 ATV.dial ASV @03:32:42.96 ASV.dial :SPErecovery @03:32:43.74 Swish #1 of 2 @03:32:44.34 AS.reconfigure ASslow0 @03:32:44.39 AS.seek 0.600ml @03:32:54.94 AS.seek 0.500ml @03:33:05.45 Swish #2 of 2 @03:33:05.52 AS.seek 0.600ml @03:33:16.07 AS.seek 0.500ml @03:33:26.57 delay 3 seconds @03:33:29.63 AS.reconfigure ASslow1 @03:33:29.68 AS.seek 0.676ml @03:34:05.87 delay 3 seconds @03:34:08.88 Eluate primed @03:34:08.89 ATV.dial :waste2 @03:34:09.42 AS.reconfigure ASnorm @03:34:09.46 AS.seek 0.000ml @03:34:30.58 Stroke completed -- waiting for pressure to equalize ... @03:34:37.71 Intake.select :close @03:34:37.85 SSV.dial :exhaust @03:34:38.62 Exhaust.select :open @03:34:38.73 SS.reconfigure SSexhale @03:34:38.78 SS.seek 0.00ml @03:34:44.32 SPE.coast @03:34:44.41 ASV.dial :SPErecovery @03:34:44.42 Setting up PCR for HF183em -- writing /var/log/esp/HF183em14jan2303h250ml.pcr @03:34:44.44 Building PCR Slug from ASV with MM2 mix, PP4 primer @03:34:44.46 Priming with carrier @03:34:44.48 ATV.dial ARV @03:34:45.19 ARV.dial :carrier @03:34:45.49 AS.seek 0.500ml @03:34:47.64 Exhaust.select :close @03:34:47.74 SSV.dial CBV @03:34:47.76 CBV.dial SSV @03:34:48.53 Allowing 185s for 25.0ml of remaining 250.0ml @03:34:48.55 Intake.select :open @03:34:50.70 Intake @23.0psia, vacuum from 16.5 to 20.5psi @03:34:50.72 SS.reconfigure forSampling @03:34:50.78 SS.seek 25.00ml @03:35:11.41 AFV.select :carrierSolution @03:35:11.52 delay 2 seconds @03:35:13.53 AS.reconfigure ASconfig @03:35:13.58 AS.seek 1.000ml @03:35:43.05 delay 1 second @03:35:44.06 AFV.select :holdingCoil @03:35:44.16 delay 1 second @03:35:45.17 ATV.dial :waste @03:35:46.42 AS.reconfigure ASnorm @03:35:46.47 AS.seek empty @03:36:37.97 AFV.select :carrierSolution @03:36:38.08 delay 2 seconds @03:36:40.09 AS.reconfigure ASconfig @03:36:40.14 AS.seek 0.500ml @03:36:41.22 Stroke completed -- waiting for pressure to equalize ... @03:36:48.87 Intake.select :close @03:36:49.01 SSV.dial :exhaust @03:36:49.79 Exhaust.select :open @03:36:49.90 SS.reconfigure SSexhale @03:36:49.95 SS.seek 0.00ml @03:36:58.82 Exhaust.select :close @03:36:58.92 SSV.dial CBV @03:36:58.94 CBV.dial SSV @03:36:59.71 Allowing 185s for 25.0ml of remaining 225.0ml @03:36:59.73 Intake.select :open @03:37:01.88 Intake @22.8psia, vacuum from 16.3 to 20.3psi @03:37:01.90 SS.reconfigure forSampling @03:37:01.96 SS.seek 25.00ml @03:37:09.64 delay 1 second @03:37:10.65 AFV.select :holdingCoil @03:37:10.75 Building Slug @03:37:10.76 ATV.dial :ARV @03:37:12.00 delay 2 seconds @03:37:14.01 ARV.dial :air1 @03:37:14.34 delay 2 seconds @03:37:16.40 AS.reconfigure ASslow2 @03:37:16.45 AS.seek 0.506ml @03:37:22.93 delay 2 seconds @03:37:24.93 ARV.dial :MM2 @03:37:26.16 delay 2 seconds @03:37:28.17 Adding MM2 @03:37:28.24 AS.seek 0.524ml @03:37:47.00 delay 25 seconds @03:38:12.01 ARV.dial :PP4 @03:38:12.36 Adding PP4 @03:38:12.38 delay 2 seconds @03:38:14.43 AS.seek 0.530ml @03:38:20.87 delay 10 seconds @03:38:30.88 ARV.dial :air3 @03:38:31.20 adding ARV air @03:38:31.77 delay 2 seconds @03:38:33.83 AS.seek 0.536ml @03:38:40.28 delay 5 seconds @03:38:45.29 ARV.dial :carrier @03:38:46.34 delay 7 seconds @03:38:52.72 Stroke completed -- waiting for pressure to equalize ... @03:38:53.40 AS.seek 0.594ml @03:39:00.45 Intake.select :close @03:39:00.60 SSV.dial :exhaust @03:39:01.37 Exhaust.select :open @03:39:01.47 SS.reconfigure SSexhale @03:39:01.52 SS.seek 0.00ml @03:39:10.37 Exhaust.select :close @03:39:10.47 SSV.dial CBV @03:39:10.49 CBV.dial SSV @03:39:11.26 Allowing 185s for 25.0ml of remaining 200.0ml @03:39:11.28 Intake.select :open @03:39:13.43 Intake @22.7psia, vacuum from 16.2 to 20.2psi @03:39:13.45 SS.reconfigure forSampling @03:39:13.51 SS.seek 25.00ml @03:39:52.90 delay 5 seconds @03:39:57.91 ATV.dial :ASV @03:39:58.23 delay 2 seconds @03:40:00.29 AS.seek 0.600ml @03:40:06.93 delay 4 seconds @03:40:10.94 ATV.dial ARV @03:40:11.28 delay 2 seconds @03:40:13.29 ARV.dial :carrier @03:40:13.30 delay 2 seconds @03:40:15.37 AS.seek 0.650ml @03:41:04.34 Stroke completed -- waiting for pressure to equalize ... @03:41:06.79 delay 4 seconds @03:41:10.84 ATV.dial PCR @03:41:11.35 ASV.dial :waste1 @03:41:12.12 Intake.select :close @03:41:12.20 ARV.dial :air1 @03:41:12.29 SSV.dial :exhaust @03:41:12.55 Positioning Slug by 0.324ml @03:41:12.62 AS.seek 0.326ml @03:41:13.05 Exhaust.select :open @03:41:13.16 SS.reconfigure SSexhale @03:41:13.21 SS.seek 0.00ml @03:41:22.22 Exhaust.select :close @03:41:22.32 SSV.dial CBV @03:41:22.34 CBV.dial SSV @03:41:23.12 Allowing 185s for 25.0ml of remaining 175.0ml @03:41:23.14 Intake.select :open @03:41:25.29 Intake @22.5psia, vacuum from 16.0 to 20.0psi @03:41:25.31 SS.reconfigure forSampling @03:41:25.37 SS.seek 25.00ml @03:43:15.86 Stroke completed -- waiting for pressure to equalize ... @03:43:24.73 Intake.select :close @03:43:24.87 SSV.dial :exhaust @03:43:25.64 Exhaust.select :open @03:43:25.74 SS.reconfigure SSexhale @03:43:25.79 SS.seek 0.00ml @03:43:34.62 Exhaust.select :close @03:43:34.72 SSV.dial CBV @03:43:34.74 CBV.dial SSV @03:43:35.50 Allowing 185s for 25.0ml of remaining 150.0ml @03:43:36.12 Intake.select :open @03:43:38.28 Intake @22.7psia, vacuum from 16.2 to 20.2psi @03:43:38.30 SS.reconfigure forSampling @03:43:38.36 SS.seek 25.00ml @03:45:28.77 Stroke completed -- waiting for pressure to equalize ... @03:45:37.74 Intake.select :close @03:45:37.89 SSV.dial :exhaust @03:45:38.65 Exhaust.select :open @03:45:38.75 SS.reconfigure SSexhale @03:45:38.80 SS.seek 0.00ml @03:45:47.73 Exhaust.select :close @03:45:47.83 SSV.dial CBV @03:45:47.85 CBV.dial SSV @03:45:48.62 Allowing 185s for 25.0ml of remaining 125.0ml @03:45:48.64 Intake.select :open @03:45:50.79 Intake @22.4psia, vacuum from 15.9 to 19.9psi @03:45:50.81 SS.reconfigure forSampling @03:45:50.87 SS.seek 25.00ml @03:46:43.28 delay 5 seconds @03:46:48.29 ATV.dial ARV @03:46:48.90 ASV.dial :PCRlock @03:46:49.25 delay 5 seconds @03:46:54.26 Running HF183em ProfileEM60C2 thermocycling @03:46:54.27 PCR.program ProfileEM60C2 @03:46:54.40 ProfileEM60C2 should complete in < 2:11:38 @03:46:54.41 PCR.run 2:49:32.5 @03:47:41.31 Stroke completed -- waiting for pressure to equalize ... @03:47:50.72 Intake.select :close @03:47:50.87 SSV.dial :exhaust @03:47:51.62 Exhaust.select :open @03:47:51.73 SS.reconfigure SSexhale @03:47:51.78 SS.seek 0.00ml @03:48:00.78 Exhaust.select :close @03:48:00.88 SSV.dial CBV @03:48:00.90 CBV.dial SSV @03:48:01.68 Allowing 185s for 25.0ml of remaining 100.0ml @03:48:01.69 Intake.select :open @03:48:03.83 Intake @22.4psia, vacuum from 15.9 to 19.9psi @03:48:03.85 SS.reconfigure forSampling @03:48:03.91 SS.seek 25.00ml @03:49:54.39 Stroke completed -- waiting for pressure to equalize ... @03:50:03.88 Intake.select :close @03:50:04.02 SSV.dial :exhaust @03:50:04.78 Exhaust.select :open @03:50:04.88 SS.reconfigure SSexhale @03:50:05.56 SS.seek 0.00ml @03:50:14.55 Exhaust.select :close @03:50:14.65 SSV.dial CBV @03:50:14.67 CBV.dial SSV @03:50:15.45 Allowing 185s for 25.0ml of remaining 75.0ml @03:50:15.46 Intake.select :open @03:50:17.61 Intake @22.4psia, vacuum from 15.9 to 19.9psi @03:50:17.63 SS.reconfigure forSampling @03:50:17.69 SS.seek 25.00ml @03:52:08.12 Stroke completed -- waiting for pressure to equalize ... @03:52:18.31 Intake.select :close @03:52:18.46 SSV.dial :exhaust @03:52:19.23 Exhaust.select :open @03:52:19.34 SS.reconfigure SSexhale @03:52:19.39 SS.seek 0.00ml @03:52:28.31 Exhaust.select :close @03:52:28.41 SSV.dial CBV @03:52:28.43 CBV.dial SSV @03:52:29.21 Allowing 185s for 25.0ml of remaining 50.0ml @03:52:29.22 Intake.select :open @03:52:31.37 Intake @22.1psia, vacuum from 15.6 to 19.6psi @03:52:31.39 SS.reconfigure forSampling @03:52:31.45 SS.seek 25.00ml @03:54:21.88 Stroke completed -- waiting for pressure to equalize ... @03:54:33.63 Intake.select :close @03:54:33.78 SSV.dial :exhaust @03:54:34.55 Exhaust.select :open @03:54:34.67 SS.reconfigure SSexhale @03:54:34.72 SS.seek 0.00ml @03:54:43.61 Exhaust.select :close @03:54:43.71 SSV.dial CBV @03:54:43.73 CBV.dial SSV @03:54:44.50 Allowing 185s for 25.0ml of remaining 25.0ml @03:54:44.51 Intake.select :open @03:54:46.66 Intake @22.1psia, vacuum from 15.6 to 19.6psi @03:54:46.68 SS.reconfigure forSampling @03:54:46.74 SS.seek 24.97ml @03:56:37.45 Stroke completed -- waiting for pressure to equalize ... @03:56:49.34 Intake.select :close @03:56:49.49 SSV.dial :exhaust @03:56:50.26 Exhaust.select :open @03:56:50.37 SS.reconfigure SSexhale @03:56:50.42 SS.seek 0.00ml @03:56:59.25 Exhaust.select :close @03:56:59.35 Sampled 1000.0ml @03:56:59.36 collectSample: Complete @03:56:59.38 Drying puck before fixing for 04:00.00 @03:56:59.39 CBV.dial :waste2b @03:57:00.17 delay 1:00 @03:58:00.17 CSV.dial :air @03:58:00.64 CS.reconfigure CSfast @03:58:00.69 CS.seek 10.000ml @03:58:23.95 CBV.dial between CSV and waste2b @03:58:24.28 Pumping wcr puck from tube 2 up to 55psi... @03:58:24.34 CBV.dial SSV @03:58:25.79 CBV.dial between CSV and waste2b @03:58:26.75 CSV.dial between air and CTV @03:58:27.15 CS.seek 6.635ml @03:58:35.59 CTV.dial CSV,avoiding => air @03:58:35.61 CSV.dial CTV @03:58:36.35 CS.reconfigure CSpressurize @03:58:36.40 CS.seek 0.000ml @03:58:51.50 CBV.dial :waste2b @03:58:51.84 CS.reconfigure CSconfig @03:58:51.89 CS.seek empty @03:58:54.98 delay 1:00 @03:59:54.99 CTV.dial :air,avoiding => intake @03:59:56.29 Fix #1 of 2 (with rnal) for 02:00.00 @03:59:56.30 CS Pulling 2ml of rnal @03:59:56.32 CSV.dial CSR @03:59:56.76 CSR.select :rnal @03:59:56.88 CS.seek 2.000ml @04:00:02.73 delay 5 seconds @04:00:07.74 CSR.select :air @04:00:07.85 CS.reconfigure CSfast @04:00:07.90 CS.seek 10.000ml @04:00:26.64 CBV.dial between CSV and waste2b @04:00:26.99 Pumping wcr puck from tube 2 up to 55psi... @04:00:27.05 CBV.dial SSV @04:00:28.49 CBV.dial between CSV and waste2b @04:00:29.45 CSV.dial between air and CTV @04:00:30.13 CS.seek 6.846ml @04:00:38.14 CTV.dial CSV,avoiding => air @04:00:38.16 CSV.dial CTV @04:00:39.47 CS.reconfigure CSpressurize @04:00:39.52 CS.seek 0.000ml @04:00:56.62 delay 2:00 @04:02:56.63 CBV.dial :waste2b @04:02:56.97 CS.reconfigure CSconfig @04:02:57.02 CS.seek empty @04:02:58.51 delay 1:00 @04:03:58.51 CTV.dial :air,avoiding => intake @04:04:00.39 Fix #2 of 2 (with rnal) for 02:00.00 @04:04:00.40 CS Pulling 2ml of rnal @04:04:00.42 CSV.dial CSR @04:04:00.87 CSR.select :rnal @04:04:00.99 CS.seek 2.000ml @04:04:06.88 delay 5 seconds @04:04:11.89 CSR.select :air @04:04:12.00 CS.reconfigure CSfast @04:04:12.05 CS.seek 10.000ml @04:04:30.95 CBV.dial between CSV and waste2b @04:04:31.30 Pumping wcr puck from tube 2 up to 55psi... @04:04:31.36 CBV.dial SSV @04:04:32.80 CBV.dial between CSV and waste2b @04:04:33.76 CSV.dial between air and CTV @04:04:34.43 CS.seek 6.846ml @04:04:42.42 CTV.dial CSV,avoiding => air @04:04:42.44 CSV.dial CTV @04:04:43.80 CS.reconfigure CSpressurize @04:04:43.85 CS.seek 0.000ml @04:05:00.40 delay 2:00 @04:07:00.41 CBV.dial :waste2b @04:07:00.73 CS.reconfigure CSconfig @04:07:00.78 CS.seek empty @04:07:02.78 delay 1:00 @04:08:02.79 CTV.dial :air,avoiding => intake @04:08:04.13 Storing wcr puck in Collection Clamp to tube 1 @04:08:04.27 SC.reconfigure SCtight @04:08:04.34 SC.seek 1 @04:08:06.60 SC.reconfigure SCloose @04:08:06.79 Elbow.reconfigure TightElbow @04:08:06.84 Elbow.seek 1 @04:08:08.44 Forearm.reconfigure TightForearm @04:08:08.49 Forearm.seek 1 + 800 counts @04:08:09.69 SE.reconfigure SEpick @04:08:09.76 SE.seek beamBreak @04:08:12.31 SE.configure SEconfig @04:08:12.45 Elbow.reconfigure LimpElbow @04:08:12.50 Forearm.reconfigure LooseForearm @04:08:12.55 Forearm.seek 1 @04:08:12.83 Elbow.hold @04:08:12.91 Elbow.reconfigure TightElbow @04:08:13.01 SE.seek 4.04 Pucks below Top Plate @04:08:13.41 Forearm.coast @04:08:13.45 Forearm.seek 1 + 340 counts @04:08:13.69 Forearm.reconfigure TightForearm @04:08:13.74 Forearm.seek 1 + 800 counts @04:08:14.01 SE.seek 5.04 Pucks below Top Plate @04:08:14.60 Forearm.reconfigure LooseForearm @04:08:14.65 Forearm.seek retract @04:08:15.63 Elbow.seek CC @04:08:17.24 Elbow.reconfigure LooseElbow @04:08:17.34 Forearm.seek CC @04:08:18.34 CC.seek sealed @04:08:19.28 Hand.grip @04:08:19.99 CC.seek open @04:08:23.36 Forearm.seek retract @04:08:24.94 Elbow.reconfigure TightElbow @04:08:24.99 Elbow.seek 1 @04:08:26.60 Elbow.reconfigure LimpElbow @04:08:26.66 Forearm.seek 1 @04:08:27.67 Elbow.hold @04:08:27.75 Elbow.reconfigure TightElbow @04:08:27.80 Hand.release @04:08:28.58 Forearm.coast @04:08:28.62 Forearm.seek 1 + 329 counts @04:08:28.88 Forearm.reconfigure TightForearm @04:08:28.93 Forearm.seek 1 + 800 counts @04:08:29.44 SE.seek down @04:08:30.55 Forearm.reconfigure LooseForearm @04:08:30.60 Forearm.seek retract @04:08:31.74 SE.coast @04:08:31.78 SC.coast @04:08:31.82 Starting finalPurge @04:08:31.83 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @04:08:32.01 Elbow.seek FlushPuck::Garage @04:08:33.78 Elbow.reconfigure LooseElbow @04:08:33.87 Forearm.seek garage @04:08:34.92 Hand.grip @04:08:35.71 Forearm.seek retract @04:08:36.73 Elbow.reconfigure TightElbow @04:08:36.78 Elbow.seek CC @04:08:39.60 Elbow.reconfigure LooseElbow @04:08:39.69 Forearm.reconfigure TightForearm @04:08:39.74 Forearm.seek CC @04:08:40.86 CC.seek closed @04:08:44.64 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.99 @04:08:44.71 Hand.release @04:08:45.53 Forearm.reconfigure LooseForearm @04:08:45.58 Forearm.seek retract @04:08:46.53 Flushing the Collection Valve::Manifold @04:08:46.65 CS Pulling 5ml of flush @04:08:46.66 CSV.dial CSR @04:08:47.17 CSR.select :flush @04:08:47.28 CS.seek 5.000ml @04:09:00.81 delay 5 seconds @04:09:05.82 CSR.select :air @04:09:05.98 CS.reconfigure CSfast @04:09:06.02 CS.seek 7.500ml @04:09:12.63 CSV.dial :waste @04:09:13.96 CS.reconfigure CSconfig @04:09:14.00 CS.seek 0.000ml @04:09:33.81 Flushing lower manifold's waste1 port @04:09:33.92 CSV.dial :flush @04:09:34.36 CS.reconfigure CSfast @04:09:34.40 CS.seek 5.000ml @04:09:46.40 delay 5 seconds @04:09:51.41 CSV.dial :air @04:09:52.12 CS.seek 7.000ml @04:09:57.43 CSV.dial CTV @04:09:57.45
CTV.dial CSV @04:09:58.79 CBV.dial :waste1 @04:09:59.57 CS.seek 0.000ml @04:10:16.02 Flushing lower manifold's waste2b port @04:10:16.70 CSV.dial :flush @04:10:17.52 CS.seek 5.000ml @04:10:29.49 delay 5 seconds @04:10:34.50 CSV.dial :air @04:10:35.08 CS.seek 7.000ml @04:10:40.40 CSV.dial CTV @04:10:40.43
CTV.dial CSV @04:10:40.88 CBV.dial :waste2b @04:10:41.66 CS.seek 0.000ml @04:10:57.99 Flushing upper manifold @04:10:58.10 CSV.dial :flush @04:10:58.91 CS.seek 5.000ml @04:11:10.93 delay 5 seconds @04:11:15.94 CSV.dial :air @04:11:16.51 CS.seek 7.000ml @04:11:21.97 CSV.dial CBV @04:11:21.99
CBV.dial CSV @04:11:23.12 CTV.dial :air @04:11:24.42 CS.seek 0.000ml @04:11:40.93 delay 7 seconds @04:11:47.94 CS.seek 3.000ml @04:11:55.52 CSV.dial :waste @04:11:56.04 CS.seek 0.000ml @04:12:03.67 CSV.dial :flush @04:12:04.11 CS.seek 3.000ml @04:12:11.67 delay 5 seconds @04:12:16.68 CSV.dial :air @04:12:17.16 CS.seek 5.000ml @04:12:22.52 CSV.dial :air @04:12:22.54 CS.seek 0.000ml @04:12:34.55 delay 7 seconds @04:12:41.56 CS.seek 3.000ml @04:12:49.08 CSV.dial :waste @04:12:49.89 CS.seek 0.000ml @04:12:57.45 Disinfecting external Intake with mfbkill @04:12:57.56 CSV.dial CSR @04:12:59.31 CSR.select :mfbkill @04:12:59.42 CS.seek 8.000ml @04:13:18.14 delay 5 seconds @04:13:23.15 CSR.select :air @04:13:23.25 CS.seek 10.000ml @04:13:28.55 CSV.dial CBV @04:13:28.58
CBV.dial CSV @04:13:29.79 CTV.dial :intake @04:13:30.50 Intake.select :open @04:13:30.61 CS.seek 2.000ml @04:13:49.67 Intake.select :close @04:13:49.78 CTV.dial :air @04:13:50.54 CS.reconfigure CSconfig @04:13:50.58 CS.seek 0.000ml @04:13:56.48 Flushing lower manifold's waste1 port @04:13:56.49 CS Pulling 4ml of mfbkill @04:13:56.51 CSV.dial CSR @04:13:57.51 CSR.select :mfbkill @04:13:57.63 CS.seek 4.000ml @04:14:08.51 delay 5 seconds @04:14:13.52 CSR.select :air @04:14:13.62 CS.reconfigure CSfast @04:14:13.67 CS.seek 10.000ml @04:14:27.90 CSV.dial CTV @04:14:27.92
CTV.dial CSV @04:14:29.27 CBV.dial :waste1 @04:14:29.72 CS.seek 0.000ml @04:14:52.82 Flushing lower manifold's waste2b port @04:14:52.83 CS Pulling 4ml of mfbkill @04:14:52.87 CSV.dial CSR @04:14:53.40 CSR.select :mfbkill @04:14:53.51 CS.reconfigure CSconfig @04:14:53.56 CS.seek 4.000ml @04:15:04.64 delay 5 seconds @04:15:09.65 CSR.select :air @04:15:09.76 CS.reconfigure CSfast @04:15:09.81 CS.seek 10.000ml @04:15:24.05 CSV.dial CTV @04:15:24.07
CTV.dial CSV @04:15:24.60 CBV.dial :waste2b @04:15:25.93 CS.seek 0.000ml @04:15:48.85 Killing upper manifold @04:15:48.86 CS Pulling 4ml of mfbkill @04:15:48.87 CSV.dial CSR @04:15:49.40 CSR.select :mfbkill @04:15:49.53 CS.reconfigure CSconfig @04:15:49.57 CS.seek 4.000ml @04:16:00.54 delay 5 seconds @04:16:05.55 CSR.select :air @04:16:05.66 CS.reconfigure CSfast @04:16:05.71 CS.seek 10.000ml @04:16:20.01 CSV.dial CBV @04:16:20.04
CBV.dial CSV @04:16:21.28 CTV.dial :air @04:16:22.58 CS.seek 0.000ml @04:16:45.91 delay 7 seconds @04:16:52.92 CS.seek 3.000ml @04:17:00.47 CSV.dial :waste @04:17:00.98 CS.seek 0.000ml @04:17:08.58 Flushing upper manifold @04:17:08.78 CSV.dial :flush @04:17:09.44 CS.seek 5.000ml @04:17:21.38 delay 5 seconds @04:17:26.39 CSV.dial :air @04:17:26.97 CS.seek 7.000ml @04:17:32.28 CSV.dial CBV @04:17:32.31
CBV.dial CSV @04:17:33.39 CTV.dial :air @04:17:33.40 CS.seek 0.000ml @04:17:49.88 delay 7 seconds @04:17:56.89 CS.seek 3.000ml @04:18:04.38 CSV.dial :waste @04:18:04.90 CS.seek 0.000ml @04:18:12.53 CSV.dial :flush @04:18:12.96 CS.seek 3.000ml @04:18:20.59 delay 5 seconds @04:18:25.60 CSV.dial :air @04:18:26.08 CS.seek 5.000ml @04:18:31.50 CSV.dial :air @04:18:31.52 CS.seek 0.000ml @04:18:43.56 delay 7 seconds @04:18:50.57 CS.seek 3.000ml @04:18:58.12 CSV.dial :waste @04:18:58.92 CS.seek 0.000ml @04:19:06.50 Flushing lower manifold's waste1 port @04:19:06.62 CSV.dial :flush @04:19:07.15 CS.seek 5.000ml @04:19:19.12 delay 5 seconds @04:19:24.13 CSV.dial :air @04:19:24.61 CS.seek 7.000ml @04:19:30.03 CSV.dial CTV @04:19:30.05
CTV.dial CSV @04:19:31.40 CBV.dial :waste1 @04:19:31.88 CS.seek 0.000ml @04:19:48.18 Flushing lower manifold's waste2b port @04:19:48.30 CSV.dial :flush @04:19:49.10 CS.seek 5.000ml @04:20:01.25 delay 5 seconds @04:20:06.26 CSV.dial :air @04:20:06.83 CS.seek 7.000ml @04:20:12.15 CSV.dial CTV @04:20:12.18
CTV.dial CSV @04:20:12.63 CBV.dial :waste2b @04:20:13.38 CS.seek 0.000ml @04:20:29.78 Disinfecting external Intake with kill @04:20:29.89 CSV.dial CSR @04:20:30.32 CSR.select :kill @04:20:30.43 CS.seek 8.000ml @04:20:49.03 delay 5 seconds @04:20:54.04 CSR.select :air @04:20:54.15 CS.seek 10.000ml @04:20:59.59 CSV.dial CBV @04:20:59.62
CBV.dial CSV @04:21:00.66 CTV.dial :intake @04:21:01.38 Intake.select :open @04:21:01.48 CS.seek 2.000ml @04:21:20.60 Intake.select :close @04:21:20.71 CTV.dial :air @04:21:21.45 CS.reconfigure CSconfig @04:21:21.49 CS.seek 0.000ml @04:21:27.47 CSV.dial CSR @04:21:28.55 CSR.select :flush @04:21:28.66 CS.seek 5.000ml @04:21:42.19 delay 5 seconds @04:21:47.20 CSR.select :air @04:21:47.31 CS.seek 10.000ml @04:22:00.78 CSV.dial CBV @04:22:01.85 CS.seek 0.000ml @04:22:27.97 Garaging FlushPuck in Collection Clamp @04:22:28.05 Elbow.reconfigure TightElbow @04:22:28.10 Elbow.seek CC @04:22:28.27 Elbow.reconfigure LooseElbow @04:22:28.36 Forearm.seek CC @04:22:29.37 CC.seek sealed @04:22:30.28 Hand.grip @04:22:31.09 CC.seek open @04:22:34.41 Forearm.seek retract @04:22:35.37 Elbow.reconfigure TightElbow @04:22:35.41 Elbow.seek garage @04:22:38.19 Elbow.reconfigure LooseElbow @04:22:38.28 Forearm.reconfigure TightForearm @04:22:38.33 Forearm.seek garage @04:22:39.53 Hand.release @04:22:40.38 Forearm.reconfigure LooseForearm @04:22:40.43 Forearm.seek retract @05:44:25.23 PCR.off @05:44:25.76 PCR for HF183em completed -- closed /var/log/esp/HF183em14jan2303h250ml.pcr @05:44:25.82 Cleaning PCR @05:44:25.83 AFV.select :carrierSolution @05:44:25.93 delay 2 seconds @05:44:27.94 AS.reconfigure ASconfig @05:44:27.99 AS.seek 0.500ml @05:44:38.45 delay 1 second @05:44:39.46 AFV.select :holdingCoil @05:44:39.56 ASV.dial :waste1 @05:44:40.32 ATV.dial :air3 @05:44:41.26 AS.reconfigure ASslow1 @05:44:41.31 AS.seek 0.510ml @05:44:43.60 delay 2 seconds @05:44:45.61 ATV.dial :bleach @05:44:46.00 AS.seek 0.810ml @05:45:47.46 delay 3 seconds @05:45:50.47 ATV.dial :air3 @05:45:50.80 delay 3 seconds @05:45:53.86 AS.seek 0.835ml @05:45:59.21 delay 3 seconds @05:46:02.22 ATV.dial :bleach @05:46:02.60 AS.seek 0.820ml @05:46:05.93 delay 3 seconds @05:46:08.94 ARV.dial :waste @05:46:09.44 delay 3 seconds @05:46:12.45 ATV.dial :ARV @05:46:13.20 AS.reconfigure ASconfig @05:46:13.25 AS.seek 0.470ml @05:46:33.95 AFV.select :carrierSolution @05:46:34.06 delay 2 seconds @05:46:36.07 AS.seek 1.000ml @05:47:07.30 delay 1 second @05:47:08.31 AFV.select :holdingCoil @05:47:08.41 delay 1 second @05:47:09.42 AS.reconfigure ASfast @05:47:09.47 AS.seek 0.000ml @05:47:37.20 AFV.select :carrierSolution @05:47:37.31 delay 2 seconds @05:47:39.32 AS.reconfigure ASconfig @05:47:39.37 AS.seek 1.000ml @05:48:37.98 delay 1 second @05:48:38.99 AFV.select :holdingCoil @05:48:39.09 delay 1 second @05:48:40.10 AS.reconfigure ASfast @05:48:40.15 AS.seek 0.000ml @05:49:07.90 AFV.select :carrierSolution @05:49:08.02 delay 2 seconds @05:49:10.03 AS.reconfigure ASconfig @05:49:10.08 AS.seek 1.000ml @05:50:08.68 delay 1 second @05:50:09.69 AFV.select :holdingCoil @05:50:09.79 delay 1 second @05:50:10.80 AS.reconfigure ASfast @05:50:10.85 AS.seek 0.000ml @05:50:38.59 ARV.dial between carrier and air1 @05:50:39.01 ATV.dial :air3 @05:50:39.95 AS.reconfigure ASslow1 @05:50:40.00 AS.seek 0.010ml @05:50:42.35 delay 2 seconds @05:50:44.36 ATV.dial :bleach @05:50:44.75 AS.seek 0.810ml @05:53:28.17 delay 3 seconds @05:53:31.18 ATV.dial :air3 @05:53:31.61 delay 3 seconds @05:53:34.67 AS.seek 0.835ml @05:53:40.03 delay 3 seconds @05:53:43.04 ATV.dial :bleach @05:53:43.54 AS.seek 0.820ml @05:53:46.88 delay 3 seconds @05:53:49.89 ATV.dial PCR @05:53:50.23 AS.reconfigure ASconfig @05:53:50.27 AS.seek empty @05:54:38.41 PCR.program :bleachProfile @05:54:38.47 PCR program should complete in < 2:45 @05:54:38.48 PCR.run 10:00 @05:57:36.68 PCR.off @05:57:36.72 AFV.select :carrierSolution @05:57:36.82 delay 2 seconds @05:57:38.83 AS.seek 1.000ml @05:58:37.51 delay 1 second @05:58:38.52 AFV.select :holdingCoil @05:58:38.62 delay 1 second @05:58:39.63 AS.reconfigure ASfast @05:58:39.68 AS.seek 0.000ml @05:59:07.40 delay 3 seconds @05:59:10.41 AFV.select :carrierSolution @05:59:10.51 delay 2 seconds @05:59:12.52 AS.reconfigure ASconfig @05:59:12.57 AS.seek 1.000ml @06:00:11.18 delay 1 second @06:00:12.19 AFV.select :holdingCoil @06:00:12.29 delay 1 second @06:00:13.30 AS.reconfigure ASfast @06:00:13.35 AS.seek 0.000ml @06:00:41.08 delay 3 seconds @06:00:44.09 AFV.select :carrierSolution @06:00:44.19 delay 2 seconds @06:00:46.20 AS.reconfigure ASconfig @06:00:46.25 AS.seek 1.000ml @06:01:44.86 delay 1 second @06:01:45.87 AFV.select :holdingCoil @06:01:45.97 delay 1 second @06:01:46.98 AS.reconfigure ASfast @06:01:47.02 AS.seek 0.000ml @06:02:14.73 delay 3 seconds @06:02:17.74 AFV.select :carrierSolution @06:02:17.84 delay 2 seconds @06:02:19.85 AS.reconfigure ASconfig @06:02:19.90 AS.seek 1.000ml @06:03:18.53 delay 1 second @06:03:19.54 AFV.select :holdingCoil @06:03:19.64 delay 1 second @06:03:20.65 AS.reconfigure ASfast @06:03:20.70 AS.seek 0.000ml @06:03:48.46 delay 3 seconds @06:03:51.47 AFV.select :carrierSolution @06:03:51.57 delay 2 seconds @06:03:53.58 AS.reconfigure ASconfig @06:03:53.63 AS.seek 1.000ml @06:04:52.27 delay 1 second @06:04:53.28 AFV.select :holdingCoil @06:04:53.38 delay 1 second @06:04:54.39 AS.reconfigure ASnorm @06:04:54.44 AS.seek 0.000ml @06:05:46.52 Setting up PCR for ENTap -- writing /var/log/esp/ENTap14jan2306h250ml.pcr @06:05:46.53 Building PCR Slug from ASV with MM1 mix, PP1 primer @06:05:46.54 Priming with carrier @06:05:46.56 ATV.dial ARV @06:05:47.19 ARV.dial :carrier @06:05:47.49 AS.seek 0.500ml @06:06:13.38 AFV.select :carrierSolution @06:06:13.49 delay 2 seconds @06:06:15.50 AS.reconfigure ASconfig @06:06:15.55 AS.seek 1.000ml @06:06:45.00 delay 1 second @06:06:46.01 AFV.select :holdingCoil @06:06:46.12 delay 1 second @06:06:47.13 ATV.dial :waste @06:06:48.48 AS.reconfigure ASnorm @06:06:48.53 AS.seek empty @06:07:40.01 AFV.select :carrierSolution @06:07:40.12 delay 2 seconds @06:07:42.13 AS.reconfigure ASconfig @06:07:42.18 AS.seek 0.500ml @06:08:11.69 delay 1 second @06:08:12.70 AFV.select :holdingCoil @06:08:12.82 Building Slug @06:08:12.83 ATV.dial :ARV @06:08:14.09 delay 2 seconds @06:08:16.10 ARV.dial :air1 @06:08:16.44 delay 2 seconds @06:08:18.50 AS.reconfigure ASslow2 @06:08:18.55 AS.seek 0.506ml @06:08:24.84 delay 2 seconds @06:08:26.85 ARV.dial :MM1 @06:08:27.36 delay 2 seconds @06:08:29.37 Adding MM1 @06:08:29.43 AS.seek 0.524ml @06:08:47.97 delay 25 seconds @06:09:12.98 ARV.dial :PP1 @06:09:13.33 Adding PP1 @06:09:13.34 delay 2 seconds @06:09:15.40 AS.seek 0.530ml @06:09:21.71 delay 10 seconds @06:09:31.72 ARV.dial :air1 @06:09:32.05 adding ARV air @06:09:32.06 delay 2 seconds @06:09:34.12 AS.seek 0.536ml @06:09:40.49 delay 5 seconds @06:09:45.50 ARV.dial :carrier @06:09:45.82 delay 7 seconds @06:09:52.88 AS.seek 0.594ml @06:10:52.51 delay 5 seconds @06:10:57.52 ATV.dial :ASV @06:10:57.87 delay 2 seconds @06:10:59.93 AS.seek 0.600ml @06:11:06.32 delay 4 seconds @06:11:10.33 ATV.dial ARV @06:11:10.68 delay 2 seconds @06:11:12.69 ARV.dial :carrier @06:11:12.70 delay 2 seconds @06:11:14.79 AS.seek 0.650ml @06:12:06.16 delay 4 seconds @06:12:10.17 ATV.dial PCR @06:12:10.68 ASV.dial :waste1 @06:12:10.69 ARV.dial :air1 @06:12:11.05 Positioning Slug by 0.324ml @06:12:11.12 AS.seek 0.326ml @06:17:41.79 delay 5 seconds @06:17:46.80 ATV.dial ARV @06:17:47.34 ASV.dial :PCRlock @06:17:47.85 delay 5 seconds @06:17:52.86 Running ENTap Profile60C thermocycling @06:17:52.87 PCR.program Profile60C @06:17:52.99 Profile60C should complete in < 1:39:08 @06:17:53.00 PCR.run 2:08:55 @07:42:27.29 PCR.off @07:42:27.82 PCR for ENTap completed -- closed /var/log/esp/ENTap14jan2306h250ml.pcr @07:42:27.88 Cleaning PCR @07:42:27.89 AFV.select :carrierSolution @07:42:27.99 delay 2 seconds @07:42:30.00 AS.reconfigure ASconfig @07:42:30.05 AS.seek 0.500ml @07:42:40.51 delay 1 second @07:42:41.52 AFV.select :holdingCoil @07:42:41.62 ASV.dial :waste1 @07:42:42.02 ATV.dial :air3 @07:42:43.06 AS.reconfigure ASslow1 @07:42:43.11 AS.seek 0.510ml @07:42:45.43 delay 2 seconds @07:42:47.44 ATV.dial :bleach @07:42:47.82 AS.seek 0.810ml @07:43:49.24 delay 3 seconds @07:43:52.25 ATV.dial :air3 @07:43:52.58 delay 3 seconds @07:43:55.64 AS.seek 0.835ml @07:44:01.01 delay 3 seconds @07:44:04.02 ATV.dial :bleach @07:44:04.40 AS.seek 0.820ml @07:44:07.74 delay 3 seconds @07:44:10.75 ARV.dial :waste @07:44:11.26 delay 3 seconds @07:44:14.27 ATV.dial :ARV @07:44:15.01 AS.reconfigure ASconfig @07:44:15.06 AS.seek 0.470ml @07:44:35.86 AFV.select :carrierSolution @07:44:35.97 delay 2 seconds @07:44:37.98 AS.seek 1.000ml @07:45:09.19 delay 1 second @07:45:10.20 AFV.select :holdingCoil @07:45:10.30 delay 1 second @07:45:11.31 AS.reconfigure ASfast @07:45:11.36 AS.seek 0.000ml @07:45:39.12 AFV.select :carrierSolution @07:45:39.23 delay 2 seconds @07:45:41.24 AS.reconfigure ASconfig @07:45:41.29 AS.seek 1.000ml @07:46:39.90 delay 1 second @07:46:40.91 AFV.select :holdingCoil @07:46:41.02 delay 1 second @07:46:42.03 AS.reconfigure ASfast @07:46:42.08 AS.seek 0.000ml @07:47:09.81 AFV.select :carrierSolution @07:47:09.92 delay 2 seconds @07:47:11.93 AS.reconfigure ASconfig @07:47:11.98 AS.seek 1.000ml @07:48:10.55 delay 1 second @07:48:11.56 AFV.select :holdingCoil @07:48:11.66 delay 1 second @07:48:12.67 AS.reconfigure ASfast @07:48:12.72 AS.seek 0.000ml @07:48:40.48 ARV.dial between carrier and air1 @07:48:40.91 ATV.dial :air3 @07:48:41.84 AS.reconfigure ASslow1 @07:48:41.89 AS.seek 0.010ml @07:48:44.24 delay 2 seconds @07:48:46.25 ATV.dial :bleach @07:48:46.63 AS.seek 0.810ml @07:51:30.05 delay 3 seconds @07:51:33.06 ATV.dial :air3 @07:51:33.48 delay 3 seconds @07:51:36.54 AS.seek 0.835ml @07:51:41.91 delay 3 seconds @07:51:44.92 ATV.dial :bleach @07:51:45.41 AS.seek 0.820ml @07:51:48.73 delay 3 seconds @07:51:51.74 ATV.dial PCR @07:51:52.09 AS.reconfigure ASconfig @07:51:52.13 AS.seek empty @07:52:40.27 PCR.program :bleachProfile @07:52:40.32 PCR program should complete in < 2:45 @07:52:40.33 PCR.run 10:00 @07:55:38.53 PCR.off @07:55:38.57 AFV.select :carrierSolution @07:55:38.67 delay 2 seconds @07:55:40.68 AS.seek 1.000ml @07:56:39.33 delay 1 second @07:56:40.34 AFV.select :holdingCoil @07:56:40.44 delay 1 second @07:56:41.45 AS.reconfigure ASfast @07:56:41.50 AS.seek 0.000ml @07:57:09.24 delay 3 seconds @07:57:12.25 AFV.select :carrierSolution @07:57:12.35 delay 2 seconds @07:57:14.36 AS.reconfigure ASconfig @07:57:14.40 AS.seek 1.000ml @07:58:13.00 delay 1 second @07:58:14.55 AFV.select :holdingCoil @07:58:14.65 delay 1 second @07:58:15.66 AS.reconfigure ASfast @07:58:15.71 AS.seek 0.000ml @07:58:43.44 delay 3 seconds @07:58:46.45 AFV.select :carrierSolution @07:58:46.55 delay 2 seconds @07:58:48.56 AS.reconfigure ASconfig @07:58:48.61 AS.seek 1.000ml @07:59:47.20 delay 1 second @07:59:48.21 AFV.select :holdingCoil @07:59:48.31 delay 1 second @07:59:49.32 AS.reconfigure ASfast @07:59:49.37 AS.seek 0.000ml @08:00:17.11 delay 3 seconds @08:00:20.12 AFV.select :carrierSolution @08:00:20.22 delay 2 seconds @08:00:22.23 AS.reconfigure ASconfig @08:00:22.28 AS.seek 1.000ml @08:01:20.89 delay 1 second @08:01:21.90 AFV.select :holdingCoil @08:01:22.00 delay 1 second @08:01:23.01 AS.reconfigure ASfast @08:01:23.06 AS.seek 0.000ml @08:01:50.78 delay 3 seconds @08:01:53.79 AFV.select :carrierSolution @08:01:53.89 delay 2 seconds @08:01:55.90 AS.reconfigure ASconfig @08:01:55.94 AS.seek 1.000ml @08:02:54.56 delay 1 second @08:02:55.57 AFV.select :holdingCoil @08:02:55.67 delay 1 second @08:02:56.68 AS.reconfigure ASnorm @08:02:56.73 AS.seek 0.000ml @08:03:48.20 Setting up PCR for ENTem -- writing /var/log/esp/ENTem14jan2308h250ml.pcr @08:03:48.21 Building PCR Slug from ASV with MM2 mix, PP3 primer @08:03:48.22 Priming with carrier @08:03:48.24 ATV.dial ARV @08:03:48.75 ARV.dial :carrier @08:03:49.07 AS.seek 0.500ml @08:04:14.95 AFV.select :carrierSolution @08:04:15.06 delay 2 seconds @08:04:17.07 AS.reconfigure ASconfig @08:04:17.12 AS.seek 1.000ml @08:04:46.58 delay 1 second @08:04:47.59 AFV.select :holdingCoil @08:04:47.69 delay 1 second @08:04:48.70 ATV.dial :waste @08:04:49.96 AS.reconfigure ASnorm @08:04:50.01 AS.seek empty @08:05:41.50 AFV.select :carrierSolution @08:05:41.61 delay 2 seconds @08:05:43.62 AS.reconfigure ASconfig @08:05:43.66 AS.seek 0.500ml @08:06:13.17 delay 1 second @08:06:14.18 AFV.select :holdingCoil @08:06:14.29 Building Slug @08:06:14.30 ATV.dial :ARV @08:06:15.55 delay 2 seconds @08:06:17.55 ARV.dial :air1 @08:06:17.89 delay 2 seconds @08:06:19.95 AS.reconfigure ASslow2 @08:06:20.00 AS.seek 0.506ml @08:06:26.26 delay 2 seconds @08:06:28.27 ARV.dial :MM2 @08:06:29.57 delay 2 seconds @08:06:31.58 Adding MM2 @08:06:31.64 AS.seek 0.524ml @08:06:50.29 delay 25 seconds @08:07:15.30 ARV.dial :PP3 @08:07:15.75 Adding PP3 @08:07:15.76 delay 2 seconds @08:07:17.82 AS.seek 0.530ml @08:07:24.29 delay 10 seconds @08:07:34.30 ARV.dial :air2 @08:07:34.66 adding ARV air @08:07:34.67 delay 2 seconds @08:07:36.73 AS.seek 0.536ml @08:07:43.13 delay 5 seconds @08:07:48.14 ARV.dial :carrier @08:07:49.18 delay 7 seconds @08:07:56.24 AS.seek 0.594ml @08:08:55.61 delay 5 seconds @08:09:00.62 ATV.dial :ASV @08:09:00.94 delay 2 seconds @08:09:03.00 AS.seek 0.600ml @08:09:09.30 delay 4 seconds @08:09:13.31 ATV.dial ARV @08:09:13.65 delay 2 seconds @08:09:15.66 ARV.dial :carrier @08:09:15.67 delay 2 seconds @08:09:17.74 AS.seek 0.650ml @08:10:08.91 delay 4 seconds @08:10:12.92 ATV.dial PCR @08:10:13.41 ASV.dial :waste1 @08:10:13.42 ARV.dial :air1 @08:10:13.78 Positioning Slug by 0.324ml @08:10:13.85 AS.seek 0.326ml @08:15:44.79 delay 5 seconds @08:15:49.80 ATV.dial ARV @08:15:50.43 ASV.dial :PCRlock @08:15:50.93 delay 5 seconds @08:15:55.94 Running ENTem ProfileEM60C thermocycling @08:15:55.95 PCR.program ProfileEM60C @08:15:56.08 ProfileEM60C should complete in < 2:11:38 @08:15:56.09 PCR.run 2:49:32.5 @10:13:41.08 PCR.off @10:13:41.62 PCR for ENTem completed -- closed /var/log/esp/ENTem14jan2308h250ml.pcr @10:13:41.68 Cleaning PCR @10:13:41.69 AFV.select :carrierSolution @10:13:41.79 delay 2 seconds @10:13:43.80 AS.reconfigure ASconfig @10:13:43.85 AS.seek 0.500ml @10:13:54.30 delay 1 second @10:13:55.31 AFV.select :holdingCoil @10:13:55.41 ASV.dial :waste1 @10:13:56.16 ATV.dial :air3 @10:13:57.09 AS.reconfigure ASslow1 @10:13:57.14 AS.seek 0.510ml @10:13:59.47 delay 2 seconds @10:14:01.48 ATV.dial :bleach @10:14:01.87 AS.seek 0.810ml @10:15:03.35 delay 3 seconds @10:15:06.36 ATV.dial :air3 @10:15:06.69 delay 3 seconds @10:15:09.75 AS.seek 0.835ml @10:15:15.10 delay 3 seconds @10:15:18.11 ATV.dial :bleach @10:15:18.49 AS.seek 0.820ml @10:15:21.81 delay 3 seconds @10:15:24.82 ARV.dial :waste @10:15:25.33 delay 3 seconds @10:15:28.34 ATV.dial :ARV @10:15:29.07 AS.reconfigure ASconfig @10:15:29.12 AS.seek 0.470ml @10:15:49.83 AFV.select :carrierSolution @10:15:49.94 delay 2 seconds @10:15:51.95 AS.seek 1.000ml @10:16:23.20 delay 1 second @10:16:24.21 AFV.select :holdingCoil @10:16:24.31 delay 1 second @10:16:25.34 AS.reconfigure ASfast @10:16:25.41 AS.seek 0.000ml @10:16:53.17 AFV.select :carrierSolution @10:16:53.28 delay 2 seconds @10:16:55.29 AS.reconfigure ASconfig @10:16:55.34 AS.seek 1.000ml @10:17:53.95 delay 1 second @10:17:54.96 AFV.select :holdingCoil @10:17:55.06 delay 1 second @10:17:56.07 AS.reconfigure ASfast @10:17:56.11 AS.seek 0.000ml @10:18:23.87 AFV.select :carrierSolution @10:18:23.99 delay 2 seconds @10:18:26.00 AS.reconfigure ASconfig @10:18:26.05 AS.seek 1.000ml @10:19:24.68 delay 1 second @10:19:25.69 AFV.select :holdingCoil @10:19:25.79 delay 1 second @10:19:26.80 AS.reconfigure ASfast @10:19:26.85 AS.seek 0.000ml @10:19:54.58 ARV.dial between carrier and air1 @10:19:55.00 ATV.dial :air3 @10:19:55.94 AS.reconfigure ASslow1 @10:19:55.99 AS.seek 0.010ml @10:19:58.33 delay 2 seconds @10:20:00.34 ATV.dial :bleach @10:20:00.72 AS.seek 0.810ml @10:22:44.06 delay 3 seconds @10:22:47.07 ATV.dial :air3 @10:22:47.40 delay 3 seconds @10:22:50.46 AS.seek 0.835ml @10:22:55.84 delay 3 seconds @10:22:58.85 ATV.dial :bleach @10:22:59.23 AS.seek 0.820ml @10:23:02.53 delay 3 seconds @10:23:05.54 ATV.dial PCR @10:23:06.54 AS.reconfigure ASconfig @10:23:06.58 AS.seek empty @10:23:54.71 PCR.program :bleachProfile @10:23:54.77 PCR program should complete in < 2:45 @10:23:54.78 PCR.run 10:00 @10:26:52.98 PCR.off @10:26:53.01 AFV.select :carrierSolution @10:26:53.11 delay 2 seconds @10:26:55.12 AS.seek 1.000ml @10:27:53.86 delay 1 second @10:27:54.87 AFV.select :holdingCoil @10:27:54.97 delay 1 second @10:27:55.98 AS.reconfigure ASfast @10:27:56.03 AS.seek 0.000ml @10:28:23.77 delay 3 seconds @10:28:26.78 AFV.select :carrierSolution @10:28:26.88 delay 2 seconds @10:28:28.89 AS.reconfigure ASconfig @10:28:28.93 AS.seek 1.000ml @10:29:27.56 delay 1 second @10:29:28.57 AFV.select :holdingCoil @10:29:28.67 delay 1 second @10:29:29.68 AS.reconfigure ASfast @10:29:29.73 AS.seek 0.000ml @10:29:57.45 delay 3 seconds @10:30:00.46 AFV.select :carrierSolution @10:30:00.56 delay 2 seconds @10:30:02.57 AS.reconfigure ASconfig @10:30:02.62 AS.seek 1.000ml @10:31:01.23 delay 1 second @10:31:02.24 AFV.select :holdingCoil @10:31:02.34 delay 1 second @10:31:03.35 AS.reconfigure ASfast @10:31:03.40 AS.seek 0.000ml @10:31:31.15 delay 3 seconds @10:31:34.16 AFV.select :carrierSolution @10:31:34.26 delay 2 seconds @10:31:36.27 AS.reconfigure ASconfig @10:31:36.31 AS.seek 1.000ml @10:32:34.93 delay 1 second @10:32:35.94 AFV.select :holdingCoil @10:32:36.04 delay 1 second @10:32:37.05 AS.reconfigure ASfast @10:32:37.10 AS.seek 0.000ml @10:33:04.82 delay 3 seconds @10:33:07.83 AFV.select :carrierSolution @10:33:07.93 delay 2 seconds @10:33:09.94 AS.reconfigure ASconfig @10:33:09.99 AS.seek 1.000ml @10:34:08.60 delay 1 second @10:34:09.61 AFV.select :holdingCoil @10:34:09.71 delay 1 second @10:34:10.72 AS.reconfigure ASnorm @10:34:10.77 AS.seek 0.000ml @10:35:02.25 Setting up PCR for IPCap -- writing /var/log/esp/IPCap14jan2310h250ml.pcr @10:35:02.26 Building PCR Slug from ASV with MM1 mix, PP2 primer @10:35:02.28 Priming with carrier @10:35:02.29 ATV.dial ARV @10:35:02.83 ARV.dial :carrier @10:35:03.13 AS.seek 0.500ml @10:35:29.03 AFV.select :carrierSolution @10:35:29.14 delay 2 seconds @10:35:31.15 AS.reconfigure ASconfig @10:35:31.20 AS.seek 1.000ml @10:36:00.66 delay 1 second @10:36:01.67 AFV.select :holdingCoil @10:36:01.77 delay 1 second @10:36:02.78 ATV.dial :waste @10:36:04.04 AS.reconfigure ASnorm @10:36:04.09 AS.seek empty @10:36:55.59 AFV.select :carrierSolution @10:36:55.70 delay 2 seconds @10:36:57.71 AS.reconfigure ASconfig @10:36:57.76 AS.seek 0.500ml @10:37:27.27 delay 1 second @10:37:28.28 AFV.select :holdingCoil @10:37:28.38 Building Slug @10:37:28.39 ATV.dial :ARV @10:37:29.65 delay 2 seconds @10:37:31.66 ARV.dial :air1 @10:37:31.99 delay 2 seconds @10:37:34.05 AS.reconfigure ASslow2 @10:37:34.10 AS.seek 0.506ml @10:37:40.37 delay 2 seconds @10:37:42.38 ARV.dial :MM1 @10:37:42.98 delay 2 seconds @10:37:44.99 Adding MM1 @10:37:45.06 AS.seek 0.524ml @10:38:03.62 delay 25 seconds @10:38:28.63 ARV.dial :PP2 @10:38:28.96 Adding PP2 @10:38:28.98 delay 2 seconds @10:38:31.04 AS.seek 0.530ml @10:38:37.36 delay 10 seconds @10:38:47.37 ARV.dial :air2 @10:38:47.80 adding ARV air @10:38:47.82 delay 2 seconds @10:38:49.87 AS.seek 0.536ml @10:38:56.36 delay 5 seconds @10:39:01.37 ARV.dial :carrier @10:39:02.40 delay 7 seconds @10:39:09.46 AS.seek 0.594ml @10:40:08.78 delay 5 seconds @10:40:13.79 ATV.dial :ASV @10:40:14.22 delay 2 seconds @10:40:16.28 AS.seek 0.600ml @10:40:22.58 delay 4 seconds @10:40:26.59 ATV.dial ARV @10:40:26.91 delay 2 seconds @10:40:28.92 ARV.dial :carrier @10:40:28.93 delay 2 seconds @10:40:31.00 AS.seek 0.650ml @10:41:22.19 delay 4 seconds @10:41:26.20 ATV.dial PCR @10:41:26.70 ASV.dial :waste1 @10:41:26.71 ARV.dial :air1 @10:41:27.07 Positioning Slug by 0.324ml @10:41:27.14 AS.seek 0.326ml @10:46:57.86 delay 5 seconds @10:47:02.87 ATV.dial ARV @10:47:03.47 ASV.dial :PCRlock @10:47:04.60 delay 5 seconds @10:47:09.61 Running IPCap Profile59C thermocycling @10:47:09.62 PCR.program Profile59C @10:47:09.74 Profile59C should complete in < 1:39:08 @10:47:09.76 PCR.run 2:08:55 @12:12:28.32 PCR.off @12:12:28.85 PCR for IPCap completed -- closed /var/log/esp/IPCap14jan2310h250ml.pcr @12:12:28.92 Cleaning PCR AFV.select :carrierSolution @12:12:29.03 delay 2 seconds @12:12:31.04 AS.reconfigure ASconfig @12:12:31.09 AS.seek 0.500ml @12:12:41.52 delay 1 second @12:12:42.53 AFV.select :holdingCoil @12:12:42.63 ASV.dial :waste1 @12:12:43.24 ATV.dial :air3 @12:12:44.27 AS.reconfigure ASslow1 @12:12:44.32 AS.seek 0.510ml @12:12:46.58 delay 2 seconds @12:12:48.59 ATV.dial :bleach @12:12:48.97 AS.seek 0.810ml @12:13:50.44 delay 3 seconds @12:13:53.45 ATV.dial :air3 @12:13:53.78 delay 3 seconds @12:13:56.84 AS.seek 0.835ml @12:14:02.20 delay 3 seconds @12:14:05.21 ATV.dial :bleach @12:14:05.60 AS.seek 0.820ml @12:14:08.91 delay 3 seconds @12:14:11.92 ARV.dial :waste @12:14:12.42 delay 3 seconds @12:14:15.43 ATV.dial :ARV @12:14:16.17 AS.reconfigure ASconfig @12:14:16.21 AS.seek 0.470ml @12:14:36.89 AFV.select :carrierSolution @12:14:36.99 delay 2 seconds @12:14:39.00 AS.seek 1.000ml @12:15:10.22 delay 1 second @12:15:11.23 AFV.select :holdingCoil @12:15:11.33 delay 1 second @12:15:12.34 AS.reconfigure ASfast @12:15:12.39 AS.seek 0.000ml @12:15:40.12 AFV.select :carrierSolution @12:15:40.24 delay 2 seconds @12:15:42.25 AS.reconfigure ASconfig @12:15:42.30 AS.seek 1.000ml @12:16:40.89 delay 1 second @12:16:41.90 AFV.select :holdingCoil @12:16:42.00 delay 1 second @12:16:43.57 AS.reconfigure ASfast @12:16:43.62 AS.seek 0.000ml @12:17:11.37 AFV.select :carrierSolution @12:17:11.48 delay 2 seconds @12:17:13.49 AS.reconfigure ASconfig @12:17:13.54 AS.seek 1.000ml @12:18:12.18 delay 1 second @12:18:13.19 AFV.select :holdingCoil @12:18:13.29 delay 1 second @12:18:14.30 AS.reconfigure ASfast @12:18:14.35 AS.seek 0.000ml @12:18:42.08 ARV.dial between carrier and air1 @12:18:42.50 ATV.dial :air3 @12:18:43.44 AS.reconfigure ASslow1 @12:18:43.49 AS.seek 0.010ml @12:18:45.78 delay 2 seconds @12:18:47.79 ATV.dial :bleach @12:18:48.18 AS.seek 0.810ml @12:21:31.54 delay 3 seconds @12:21:34.55 ATV.dial :air3 @12:21:34.98 delay 3 seconds @12:21:38.04 AS.seek 0.835ml @12:21:43.38 delay 3 seconds @12:21:46.39 ATV.dial :bleach @12:21:46.89 AS.seek 0.820ml @12:21:50.20 delay 3 seconds @12:21:53.21 ATV.dial PCR @12:21:53.55 AS.reconfigure ASconfig @12:21:53.59 AS.seek empty @12:22:41.70 PCR.program :bleachProfile @12:22:41.76 PCR program should complete in < 2:45 @12:22:41.77 PCR.run 10:00 @12:25:39.97 PCR.off @12:25:40.01 AFV.select :carrierSolution @12:25:40.11 delay 2 seconds @12:25:42.12 AS.seek 1.000ml @12:26:40.77 delay 1 second @12:26:41.78 AFV.select :holdingCoil @12:26:41.88 delay 1 second @12:26:42.89 AS.reconfigure ASfast @12:26:42.94 AS.seek 0.000ml @12:27:10.67 delay 3 seconds @12:27:13.68 AFV.select :carrierSolution @12:27:13.78 delay 2 seconds @12:27:15.79 AS.reconfigure ASconfig @12:27:15.83 AS.seek 1.000ml @12:28:14.43 delay 1 second @12:28:15.44 AFV.select :holdingCoil @12:28:15.54 delay 1 second @12:28:16.55 AS.reconfigure ASfast @12:28:16.60 AS.seek 0.000ml @12:28:44.32 delay 3 seconds @12:28:47.33 AFV.select :carrierSolution @12:28:47.43 delay 2 seconds @12:28:49.44 AS.reconfigure ASconfig @12:28:49.49 AS.seek 1.000ml @12:29:48.12 delay 1 second @12:29:49.13 AFV.select :holdingCoil @12:29:49.23 delay 1 second @12:29:50.24 AS.reconfigure ASfast @12:29:50.32 AS.seek 0.000ml @12:30:18.07 delay 3 seconds @12:30:21.08 AFV.select :carrierSolution @12:30:21.18 delay 2 seconds @12:30:23.19 AS.reconfigure ASconfig @12:30:23.24 AS.seek 1.000ml @12:31:21.83 delay 1 second @12:31:22.84 AFV.select :holdingCoil @12:31:22.94 delay 1 second @12:31:23.95 AS.reconfigure ASfast @12:31:24.00 AS.seek 0.000ml @12:31:51.74 delay 3 seconds @12:31:54.75 AFV.select :carrierSolution @12:31:54.85 delay 2 seconds @12:31:56.86 AS.reconfigure ASconfig @12:31:56.90 AS.seek 1.000ml @12:32:55.50 delay 1 second @12:32:56.51 AFV.select :holdingCoil @12:32:56.61 delay 1 second @12:32:57.62 AS.reconfigure ASnorm @12:32:57.66 AS.seek 0.000ml @12:33:49.14 Setting up PCR for IPCem -- writing /var/log/esp/IPCem14jan2312h250ml.pcr @12:33:49.15 Building PCR Slug from ASV with MM3 mix, PP5 primer @12:33:49.17 Priming with carrier @12:33:49.18 ATV.dial ARV @12:33:49.70 ARV.dial :carrier @12:33:50.00 AS.seek 0.500ml @12:34:15.91 AFV.select :carrierSolution @12:34:16.02 delay 2 seconds @12:34:18.03 AS.reconfigure ASconfig @12:34:18.08 AS.seek 1.000ml @12:34:47.55 delay 1 second @12:34:48.56 AFV.select :holdingCoil @12:34:48.66 delay 1 second @12:34:49.67 ATV.dial :waste @12:34:50.92 AS.reconfigure ASnorm @12:34:50.97 AS.seek empty @12:35:42.46 AFV.select :carrierSolution @12:35:42.57 delay 2 seconds @12:35:44.58 AS.reconfigure ASconfig @12:35:44.63 AS.seek 0.500ml @12:36:14.13 delay 1 second @12:36:15.14 AFV.select :holdingCoil @12:36:15.25 Building Slug @12:36:15.26 ATV.dial :ARV @12:36:16.51 delay 2 seconds @12:36:18.52 ARV.dial :air1 @12:36:18.85 delay 2 seconds @12:36:20.91 AS.reconfigure ASslow2 @12:36:20.96 AS.seek 0.506ml @12:36:27.26 delay 2 seconds @12:36:29.27 ARV.dial :MM3 @12:36:30.13 delay 2 seconds @12:36:32.14 Adding MM3 @12:36:32.21 AS.seek 0.524ml @12:36:50.71 delay 25 seconds @12:37:15.72 ARV.dial :PP5 @12:37:16.16 Adding PP5 @12:37:16.18 delay 2 seconds @12:37:18.24 AS.seek 0.530ml @12:37:24.52 delay 10 seconds @12:37:34.53 ARV.dial :air3 @12:37:34.86 adding ARV air @12:37:34.87 delay 2 seconds @12:37:36.93 AS.seek 0.536ml @12:37:43.77 delay 5 seconds @12:37:48.78 ARV.dial :carrier @12:37:49.84 delay 7 seconds @12:37:56.89 AS.seek 0.594ml @12:38:56.47 delay 5 seconds @12:39:01.48 ATV.dial :ASV @12:39:01.81 delay 2 seconds @12:39:03.87 AS.seek 0.600ml @12:39:10.39 delay 4 seconds @12:39:14.40 ATV.dial ARV @12:39:14.75 delay 2 seconds @12:39:16.76 ARV.dial :carrier @12:39:16.77 delay 2 seconds @12:39:18.84 AS.seek 0.650ml @12:40:10.14 delay 4 seconds @12:40:14.15 ATV.dial PCR @12:40:14.65 ASV.dial :waste1 @12:40:14.66 ARV.dial :air1 @12:40:15.02 Positioning Slug by 0.324ml @12:40:15.09 AS.seek 0.326ml @12:45:45.75 delay 5 seconds @12:45:50.76 ATV.dial ARV @12:45:51.36 ASV.dial :PCRlock @12:45:51.71 delay 5 seconds @12:45:56.72 Running IPCem ProfileEM59C thermocycling @12:45:56.73 PCR.program ProfileEM59C @12:45:56.86 ProfileEM59C should complete in < 2:11:38 @12:45:56.87 PCR.run 2:49:32.5 @14:43:53.46 PCR.off @14:43:53.99 PCR for IPCem completed -- closed /var/log/esp/IPCem14jan2312h250ml.pcr @14:43:54.01 Full MFB Decontamination @14:43:54.02 Decon extraction column and decon AIV @14:43:54.05 SPE.hold 2:46:40,55.0C @14:43:54.07 ASV.dial :SPEdelivery @14:43:54.78 delay 20 seconds @14:44:14.79 AFV.select :carrierSolution @14:44:14.89 delay 2 seconds @14:44:16.90 AS.reconfigure ASconfig @14:44:16.95 AS.seek 0.500ml @14:44:27.41 delay 1 second @14:44:28.42 AFV.select :holdingCoil @14:44:28.52 ATV.dial :air1 @14:44:30.07 AS.reconfigure ASslow1 @14:44:30.12 AS.seek 0.520ml @14:44:35.02 ATV.dial :ASV @14:44:36.42 ASV.dial :SPErecovery @14:44:36.99 AS.seek 0.620ml @14:44:57.64 ATV.dial :waste @14:44:59.14 AS.reconfigure ASnorm @14:44:59.19 AS.seek empty @14:45:31.25 AFV.select :holdingCoil @14:45:31.26 ATV.dial :waste @14:45:31.28 AS.reconfigure ASconfig @14:45:31.34 AS.seek 0.000ml @14:45:31.64 AFV.select :carrierSolution @14:45:31.75 delay 2 seconds @14:45:33.76 AS.seek 0.500ml @14:46:03.19 delay 1 second @14:46:04.20 AFV.select :holdingCoil @14:46:04.30 ATV.dial :air3 @14:46:04.95 AS.reconfigure ASslow1 @14:46:05.00 AS.seek 0.520ml @14:46:09.36 ATV.dial :air3 @14:46:09.50 AS.seek 0.530ml @14:46:11.78 delay 2 seconds @14:46:13.79 ATV.dial :bleach @14:46:14.17 AS.seek 0.710ml @14:46:51.17 delay 3 seconds @14:46:54.18 ATV.dial :air3 @14:46:54.51 delay 3 seconds @14:46:57.57 AS.seek 0.735ml @14:47:02.95 delay 3 seconds @14:47:05.96 ATV.dial :bleach @14:47:06.34 AS.seek 0.720ml @14:47:09.62 delay 3 seconds @14:47:12.63 ATV.dial ASV @14:47:13.51 ASV.dial :SPErecovery @14:47:13.59 AS.seek 0.365ml @14:48:26.24 ATV.dial :waste @14:48:27.69 AS.reconfigure ASnorm @14:48:27.74 AS.seek empty @14:48:46.79 ATV.dial :air3 @14:48:47.37 AS.reconfigure ASslow1 @14:48:47.42 AS.seek 0.009ml @14:48:49.79 delay 2 seconds @14:48:51.80 ATV.dial :bleach @14:48:52.17 AS.seek 0.579ml @14:50:48.74 delay 3 seconds @14:50:51.75 ATV.dial :air3 @14:50:52.08 delay 3 seconds @14:50:55.14 AS.seek 0.604ml @14:51:00.54 delay 3 seconds @14:51:03.55 ATV.dial :bleach @14:51:03.93 AS.seek 0.589ml @14:51:07.22 delay 3 seconds @14:51:10.23 ATV.dial :ASV @14:51:11.11 ASV.dial :wasteR @14:51:11.61 AS.seek 0.000ml @14:53:12.06 delay 1:00 @14:54:12.07 ATV.dial ASV @14:54:12.08 AFV.select :carrierSolution @14:54:12.18 delay 2 seconds @14:54:14.19 AS.reconfigure ASconfig @14:54:14.23 AS.seek 1.000ml @14:55:12.85 delay 1 second @14:55:13.86 AFV.select :holdingCoil @14:55:13.96 delay 1 second @14:55:14.97 ASV.dial :wasteR @14:55:14.98 AS.reconfigure ASslow1 @14:55:15.03 AS.seek 0.000ml @14:58:39.29 delay 10 seconds @14:58:49.30 ATV.dial :air3 @14:58:50.42 AS.seek 0.010ml @14:58:52.78 delay 2 seconds @14:58:54.79 ATV.dial :bleach @14:58:55.17 AS.seek 0.580ml @15:00:51.70 delay 3 seconds @15:00:54.71 ATV.dial :air3 @15:00:55.15 delay 3 seconds @15:00:58.21 AS.seek 0.605ml @15:01:03.57 delay 3 seconds @15:01:06.58 ATV.dial :bleach @15:01:06.97 AS.seek 0.590ml @15:01:10.31 delay 3 seconds @15:01:13.32 ATV.dial ASV @15:01:14.20 ASV.dial :SPEdelivery @15:01:14.82 AS.seek 0.000ml @15:03:15.36 delay 1:00 @15:04:15.37 AFV.select :carrierSolution @15:04:15.47 delay 2 seconds @15:04:17.48 AS.reconfigure ASconfig @15:04:17.53 AS.seek 1.000ml @15:05:16.16 delay 1 second @15:05:17.17 AFV.select :holdingCoil @15:05:17.28 delay 1 second @15:05:18.29 ATV.dial ASV @15:05:18.30 ASV.dial :SPEdelivery @15:05:18.32 AS.reconfigure ASslow1 @15:05:18.37 AS.seek 0.000ml @15:08:42.60 delay 10 seconds @15:08:52.61 ATV.dial :air2 @15:08:53.33 AS.reconfigure ASslow0 @15:08:53.38 AS.seek 0.800ml @15:10:15.33 delay 3 seconds @15:10:18.34 ATV.dial :ASV @15:10:19.05 ASV.dial :SPEdelivery @15:10:19.07 AS.reconfigure ASslow1 @15:10:19.12 AS.seek 0.030ml @15:12:56.43 delay 20 seconds @15:13:16.44 ATV.dial :waste,via => air3 @15:13:17.88 AS.reconfigure ASconfig @15:13:17.92 AS.seek empty @15:13:19.99 SPE.coast @15:13:20.07 Removing Bleach from Mixing Coil @15:13:20.08 AFV.select :carrierSolution @15:13:20.19 delay 2 seconds @15:13:22.20 AS.seek 0.500ml @15:13:51.71 delay 1 second @15:13:52.72 AFV.select :holdingCoil @15:13:52.82 ATV.dial :ASV @15:13:54.35 ASV.dial :SPErecovery @15:13:55.18 AS.reconfigure ASslow1 @15:13:55.23 AS.seek 0.730ml @15:14:42.40 ASV.dial :wasteD @15:14:43.63 AS.reconfigure ASnorm @15:14:43.68 AS.seek 0.000ml @15:15:21.30 AFV.select :carrierSolution @15:15:21.41 delay 2 seconds @15:15:23.42 AS.reconfigure ASconfig @15:15:23.47 AS.seek 0.500ml @15:15:52.91 delay 1 second @15:15:53.92 AFV.select :holdingCoil @15:15:54.02 ATV.dial ASV @15:15:54.03 ASV.dial :SPErecovery @15:15:54.76 AS.reconfigure ASslow0 @15:15:54.81 AS.seek 0.300ml @15:16:15.52 ATV.dial :air2 @15:16:16.27 AS.reconfigure ASslow1 @15:16:16.32 AS.seek 0.320ml @15:16:20.68 ATV.dial ASV @15:16:21.46 AS.reconfigure ASslow0 @15:16:21.51 AS.seek 0.570ml @15:16:47.27 ASV.dial :wasteD @15:16:47.95 AS.reconfigure ASnorm @15:16:48.00 AS.seek 0.000ml @15:17:17.44 AFV.select :carrierSolution @15:17:17.55 delay 2 seconds @15:17:19.56 AS.reconfigure ASconfig @15:17:19.61 AS.seek 0.500ml @15:17:49.06 delay 1 second @15:17:50.07 AFV.select :holdingCoil @15:17:50.18 ATV.dial ASV @15:17:50.19 ASV.dial :SPErecovery @15:17:50.91 AS.reconfigure ASslow0 @15:17:50.96 AS.seek 0.300ml @15:18:11.67 ATV.dial :air2 @15:18:12.44 AS.reconfigure ASslow1 @15:18:12.49 AS.seek 0.320ml @15:18:16.86 ATV.dial ASV @15:18:17.72 AS.reconfigure ASslow0 @15:18:17.76 AS.seek 0.570ml @15:18:43.59 ASV.dial :wasteD @15:18:44.54 AS.reconfigure ASnorm @15:18:44.59 AS.seek 0.000ml @15:19:14.04 ATV.dial :air2 @15:19:14.74 AS.reconfigure ASslow0 @15:19:14.78 AS.seek 0.300ml @15:19:45.73 ATV.dial :ASV @15:19:46.45 ASV.dial :wasteD @15:19:46.47 AS.reconfigure ASconfig @15:19:46.52 AS.seek 0.050ml @15:20:01.42 ATV.dial :waste @15:20:02.85 AS.seek 0.000ml @15:20:06.03 ASV.dial :SPEdelivery @15:20:06.89 deconLysateINT @15:20:06.90 ATV.dial :air3 @15:20:07.47 AS.reconfigure ASslow1 @15:20:07.52 AS.seek 0.010ml @15:20:09.87 delay 2 seconds @15:20:11.88 ATV.dial :bleach @15:20:12.26 AS.seek 0.960ml @15:23:26.27 delay 3 seconds @15:23:29.28 ATV.dial :air3 @15:23:29.63 delay 3 seconds @15:23:32.69 AS.seek 0.985ml @15:23:38.07 delay 3 seconds @15:23:41.08 ATV.dial :bleach @15:23:41.57 AS.seek 0.970ml @15:23:44.89 delay 3 seconds @15:23:47.90 ATV.dial :AIV @15:23:48.97 AIV.dial :coilToMFB @15:23:48.98 AWV.select :waste @15:23:49.00 AFV.select :carrierSolution @15:23:49.11 delay 2 seconds @15:23:51.12 AS.reconfigure ASconfig @15:23:51.17 AS.seek 1.000ml @15:23:53.27 delay 1 second @15:23:54.28 AFV.select :holdingCoil @15:23:54.39 delay 1 second @15:23:55.40 AS.reconfigure ASslow0 @15:23:55.45 AS.seek empty @15:25:37.89 AFV.select :carrierSolution @15:25:38.00 delay 2 seconds @15:25:40.01 AS.reconfigure ASconfig @15:25:40.06 AS.seek 1.000ml @15:26:38.73 delay 1 second @15:26:39.74 AFV.select :holdingCoil @15:26:39.84 delay 1 second @15:26:40.85 AS.reconfigure ASslow0 @15:26:40.90 AS.seek empty @15:28:23.32 AFV.select :carrierSolution @15:28:23.43 delay 2 seconds @15:28:25.44 AS.reconfigure ASconfig @15:28:25.49 AS.seek 1.000ml @15:29:24.17 delay 1 second @15:29:25.18 AFV.select :holdingCoil @15:29:25.29 delay 1 second @15:29:26.30 AS.reconfigure ASslow0 @15:29:26.35 AS.seek empty @15:31:08.76 ATV.dial :air2 @15:31:09.33 AS.reconfigure ASslow1 @15:31:09.38 AS.seek 0.009ml @15:31:11.72 AS.reconfigure ASslow0 @15:31:11.77 AS.seek 1.000ml @15:32:53.10 ATV.dial AIV @15:32:53.63 AS.seek 0.020ml @15:34:33.93 ATV.dial :waste @15:34:35.20 AS.seek 0.000ml @15:34:37.52 AWV.select :air @15:34:37.67 Cleaning PCR @15:34:37.68 AFV.select :carrierSolution @15:34:37.78 delay 2 seconds @15:34:39.79 AS.reconfigure ASconfig @15:34:39.84 AS.seek 0.500ml @15:35:09.30 delay 1 second @15:35:10.31 AFV.select :holdingCoil @15:35:10.41 ASV.dial :waste1 @15:35:10.74 ATV.dial :air3 @15:35:11.31 AS.reconfigure ASslow1 @15:35:11.35 AS.seek 0.510ml @15:35:13.66 delay 2 seconds @15:35:15.67 ATV.dial :bleach @15:35:16.06 AS.seek 0.810ml @15:36:18.07 delay 3 seconds @15:36:21.08 ATV.dial :air3 @15:36:21.41 delay 3 seconds @15:36:24.47 AS.seek 0.835ml @15:36:29.83 delay 3 seconds @15:36:32.84 ATV.dial :bleach @15:36:33.23 AS.seek 0.820ml @15:36:36.55 delay 3 seconds @15:36:39.56 ARV.dial :waste @15:36:40.07 delay 3 seconds @15:36:43.08 ATV.dial :ARV @15:36:43.91 AS.reconfigure ASconfig @15:36:43.96 AS.seek 0.470ml @15:37:04.66 AFV.select :carrierSolution @15:37:04.77 delay 2 seconds @15:37:06.78 AS.seek 1.000ml @15:37:38.02 delay 1 second @15:37:39.03 AFV.select :holdingCoil @15:37:39.13 delay 1 second @15:37:40.14 AS.reconfigure ASfast @15:37:40.19 AS.seek 0.000ml @15:38:07.92 AFV.select :carrierSolution @15:38:08.03 delay 2 seconds @15:38:10.04 AS.reconfigure ASconfig @15:38:10.09 AS.seek 1.000ml @15:39:08.67 delay 1 second @15:39:09.68 AFV.select :holdingCoil @15:39:09.78 delay 1 second @15:39:10.79 AS.reconfigure ASfast @15:39:10.84 AS.seek 0.000ml @15:39:38.58 AFV.select :carrierSolution @15:39:38.69 delay 2 seconds @15:39:40.70 AS.reconfigure ASconfig @15:39:40.75 AS.seek 1.000ml @15:40:39.35 delay 1 second @15:40:40.36 AFV.select :holdingCoil @15:40:40.47 delay 1 second @15:40:41.48 AS.reconfigure ASfast @15:40:41.53 AS.seek 0.000ml @15:41:09.28 ARV.dial between carrier and air1 @15:41:09.71 ATV.dial :air3 @15:41:10.65 AS.reconfigure ASslow1 @15:41:10.70 AS.seek 0.010ml @15:41:13.06 delay 2 seconds @15:41:15.07 ATV.dial :bleach @15:41:15.47 AS.seek 0.810ml @15:43:58.89 delay 3 seconds @15:44:01.90 ATV.dial :air3 @15:44:02.23 delay 3 seconds @15:44:05.29 AS.seek 0.835ml @15:44:10.65 delay 3 seconds @15:44:13.66 ATV.dial :bleach @15:44:14.05 AS.seek 0.820ml @15:44:17.36 delay 3 seconds @15:44:20.37 ATV.dial PCR @15:44:20.71 AS.reconfigure ASconfig @15:44:20.75 AS.seek empty @15:45:08.86 PCR.program :bleachProfile @15:45:08.92 PCR program should complete in < 2:45 @15:45:08.93 PCR.run 10:00 @15:48:10.15 PCR.off @15:48:10.18 AFV.select :carrierSolution @15:48:10.28 delay 2 seconds @15:48:12.29 AS.seek 1.000ml @15:49:10.94 delay 1 second @15:49:11.95 AFV.select :holdingCoil @15:49:12.05 delay 1 second @15:49:13.06 AS.reconfigure ASfast @15:49:13.11 AS.seek 0.000ml @15:49:40.83 delay 3 seconds @15:49:43.84 AFV.select :carrierSolution @15:49:43.94 delay 2 seconds @15:49:45.95 AS.reconfigure ASconfig @15:49:46.00 AS.seek 1.000ml @15:50:44.61 delay 1 second @15:50:45.62 AFV.select :holdingCoil @15:50:45.76 delay 1 second @15:50:46.77 AS.reconfigure ASfast @15:50:46.81 AS.seek 0.000ml @15:51:14.55 delay 3 seconds @15:51:17.56 AFV.select :carrierSolution @15:51:17.67 delay 2 seconds @15:51:19.68 AS.reconfigure ASconfig @15:51:19.73 AS.seek 1.000ml @15:52:18.36 delay 1 second @15:52:19.37 AFV.select :holdingCoil @15:52:19.47 delay 1 second @15:52:20.48 AS.reconfigure ASfast @15:52:20.53 AS.seek 0.000ml @15:52:48.26 delay 3 seconds @15:52:51.27 AFV.select :carrierSolution @15:52:51.37 delay 2 seconds @15:52:53.38 AS.reconfigure ASconfig @15:52:53.42 AS.seek 1.000ml @15:53:52.02 delay 1 second @15:53:53.03 AFV.select :holdingCoil @15:53:53.13 delay 1 second @15:53:54.14 AS.reconfigure ASfast @15:53:54.19 AS.seek 0.000ml @15:54:21.93 delay 3 seconds @15:54:24.94 AFV.select :carrierSolution @15:54:25.04 delay 2 seconds @15:54:27.05 AS.reconfigure ASconfig @15:54:27.10 AS.seek 1.000ml @15:55:25.72 delay 1 second @15:55:26.73 AFV.select :holdingCoil @15:55:26.83 delay 1 second @15:55:27.84 AS.reconfigure ASnorm @15:55:27.89 AS.seek 0.000ml @15:56:19.38 AFV.select :carrierSolution @15:56:19.48 delay 2 seconds @15:56:21.49 AS.reconfigure ASconfig @15:56:21.54 AS.seek 1.000ml @15:57:20.16 delay 1 second @15:57:21.17 AFV.select :holdingCoil @15:57:21.27 delay 1 second @15:57:22.28 ATV.dial :waste @15:57:23.18 AS.reconfigure ASnorm @15:57:23.22 AS.seek 0.500ml @15:57:49.16 ATV.dial :waste2 @15:57:50.25 AS.seek 0.000ml @15:58:16.14
Cleaning PCR AFV.select :carrierSolution @15:58:16.24 delay 2 seconds @15:58:18.25 AS.reconfigure ASconfig @15:58:18.30 AS.seek 0.500ml @15:58:47.75 delay 1 second @15:58:48.76 AFV.select :holdingCoil @15:58:48.86 ASV.dial :waste1 @15:58:48.87 ATV.dial :air3 @15:58:50.81 AS.reconfigure ASslow1 @15:58:50.85 AS.seek 0.510ml @15:58:53.16 delay 2 seconds @15:58:55.17 ATV.dial :bleach @15:58:55.56 AS.seek 0.810ml @15:59:57.03 delay 3 seconds @16:00:00.04 ATV.dial :air3 @16:00:00.48 delay 3 seconds @16:00:03.55 AS.seek 0.835ml @16:00:08.93 delay 3 seconds @16:00:11.94 ATV.dial :bleach @16:00:12.34 AS.seek 0.820ml @16:00:15.65 delay 3 seconds @16:00:18.66 ARV.dial :waste @16:00:19.07 delay 3 seconds @16:00:22.08 ATV.dial :ARV @16:00:22.82 AS.reconfigure ASconfig @16:00:22.86 AS.seek 0.470ml @16:00:43.55 AFV.select :carrierSolution @16:00:43.65 delay 2 seconds @16:00:45.67 AS.seek 1.000ml @16:01:16.93 delay 1 second @16:01:17.94 AFV.select :holdingCoil @16:01:18.04 delay 1 second @16:01:19.06 AS.reconfigure ASfast @16:01:19.11 AS.seek 0.000ml @16:01:46.86 AFV.select :carrierSolution @16:01:46.96 delay 2 seconds @16:01:48.98 AS.reconfigure ASconfig @16:01:49.03 AS.seek 1.000ml @16:02:47.66 delay 1 second @16:02:48.67 AFV.select :holdingCoil @16:02:48.77 delay 1 second @16:02:49.79 AS.reconfigure ASfast @16:02:49.84 AS.seek 0.000ml @16:03:17.55 AFV.select :carrierSolution @16:03:17.65 delay 2 seconds @16:03:19.67 AS.reconfigure ASconfig @16:03:19.72 AS.seek 1.000ml @16:04:18.33 delay 1 second @16:04:19.34 AFV.select :holdingCoil @16:04:19.44 delay 1 second @16:04:20.46 AS.reconfigure ASfast @16:04:20.51 AS.seek 0.000ml @16:04:48.23 ARV.dial between carrier and air1 @16:04:48.65 ATV.dial :air3 @16:04:49.59 AS.reconfigure ASslow1 @16:04:49.63 AS.seek 0.010ml @16:04:51.98 delay 2 seconds @16:04:53.99 ATV.dial :bleach @16:04:54.37 AS.seek 0.810ml @16:07:37.79 delay 3 seconds @16:07:40.80 ATV.dial :air3 @16:07:41.23 delay 3 seconds @16:07:44.29 AS.seek 0.835ml @16:07:49.63 delay 3 seconds @16:07:52.64 ATV.dial :bleach @16:07:53.03 AS.seek 0.820ml @16:07:56.35 delay 3 seconds @16:07:59.36 ATV.dial PCR @16:07:59.70 AS.reconfigure ASconfig @16:07:59.74 AS.seek empty @16:08:47.85 PCR.program :bleachProfile @16:08:47.91 PCR program should complete in < 2:45 @16:08:47.92 PCR.run 10:00 @16:11:49.14 PCR.off @16:11:49.17 AFV.select :carrierSolution @16:11:49.27 delay 2 seconds @16:11:51.29 AS.seek 1.000ml @16:12:49.98 delay 1 second @16:12:50.99 AFV.select :holdingCoil @16:12:51.09 delay 1 second @16:12:52.11 AS.reconfigure ASfast @16:12:52.16 AS.seek 0.000ml @16:13:19.87 delay 3 seconds @16:13:22.88 AFV.select :carrierSolution @16:13:22.98 delay 2 seconds @16:13:25.00 AS.reconfigure ASconfig @16:13:25.09 AS.seek 1.000ml @16:14:23.71 delay 1 second @16:14:24.72 AFV.select :holdingCoil @16:14:24.82 delay 1 second @16:14:25.83 AS.reconfigure ASfast @16:14:25.88 AS.seek 0.000ml @16:14:53.62 delay 3 seconds @16:14:56.63 AFV.select :carrierSolution @16:14:56.73 delay 2 seconds @16:14:58.74 AS.reconfigure ASconfig @16:14:58.79 AS.seek 1.000ml @16:15:57.40 delay 1 second @16:15:58.41 AFV.select :holdingCoil @16:15:58.51 delay 1 second @16:15:59.53 AS.reconfigure ASfast @16:15:59.58 AS.seek 0.000ml @16:16:27.33 delay 3 seconds @16:16:30.34 AFV.select :carrierSolution @16:16:30.44 delay 2 seconds @16:16:32.45 AS.reconfigure ASconfig @16:16:32.50 AS.seek 1.000ml @16:17:31.12 delay 1 second @16:17:32.13 AFV.select :holdingCoil @16:17:32.23 delay 1 second @16:17:33.25 AS.reconfigure ASfast @16:17:33.30 AS.seek 0.000ml @16:18:01.00 delay 3 seconds @16:18:04.01 AFV.select :carrierSolution @16:18:04.11 delay 2 seconds @16:18:06.12 AS.reconfigure ASconfig @16:18:06.17 AS.seek 1.000ml @16:19:04.84 delay 1 second @16:19:05.84 AFV.select :holdingCoil @16:19:05.94 delay 1 second @16:19:06.95 AS.reconfigure ASnorm @16:19:07.00 AS.seek 0.000ml @16:19:58.43 Completed {wqPCR} begun 01:49:41 @16:19:58.47 Asleep til +0:10 @16:19:58.48 AS.stop @16:19:58.51
Sleeping until 16:20 @16:19:58.53 GoodNews.email "Then, in tube 2: coreNegNTCphase\n/home/esp/esp2/mission/14jan21SCCWRP.rb:57",Subject => "Asleep until 16:20" @16:20:00.59 CC.stop @16:20:00.63 CS.stop @16:20:00.67 PC.stop @16:20:00.71 PS.stop @16:20:00.74 Forearm.stop @16:20:01.26 Elbow.stop @16:20:01.30 SS.stop @16:20:01.37 Sleepy.power :raw,:OFF @16:20:01.38 Sleepy.power :dwarf,:OFF @16:20:01.39 Shutdown @16:20:08.76
Powering up Dwarves @16:20:13.64 Sleepy.power :raw,:ON @16:20:14.10 SPE.configure SPEconfig @16:20:14.13 AS.configure ASconfig @16:20:14.18 CC.configure CCconfig @16:20:14.22 CS.configure CSconfig @16:20:14.26 PC.configure PCconfig @16:20:14.30 PS.configure PSconfig @16:20:14.35 Forearm.configure LooseForearm @16:20:14.39 Elbow.configure LooseElbow @16:20:14.43 SC.configure SCloose @16:20:14.47 SE.configure SEconfig @16:20:14.51 SS.configure SSconfig @16:20:14.55 CH.configure CHconfig @16:20:14.58 PH.configure PHconfig @16:20:14.62 Valve.reset @16:20:15.14 PCR.off @16:20:15.18 PCR.pidtemp= 50,0.1,0,300,25,25 @16:20:15.21 PCR.caltemp= 6.6661,0.0344,0.0 @16:20:15.33 Intake.select :close @16:20:15.34 Exhaust.select :close @16:20:15.35 CTV.dial :air @16:20:15.41 PSV.dial :air @16:20:15.45
PC.reconfigure PCconfig @16:20:15.52 CSV.dial :waste1 @16:20:15.54
PC.home.seek open @16:20:15.93 PS.reconfigure PSconfig @16:20:15.98 PS.home.seek 0.000ml @16:20:16.00 SSV.dial :spit,avoiding => exhaust @16:20:16.81 CS.reconfigure CSfast @16:20:16.84 SS.reconfigure SSconfig @16:20:16.95 SS.home.seek 0.00ml @16:20:16.97 CS.home.seek 0.000ml @16:20:17.06 CC.reconfigure CCconfig @16:20:17.11 CC.home.seek open @16:20:26.28 PS.coast @16:20:26.37
CC.seek open @16:20:26.40 PC.seek open @16:20:26.55
Forearm.reconfigure LooseForearm @16:20:26.60 Forearm.home.seek retract @16:20:27.35 Hand.grip @16:20:27.38
Elbow.jog -1500 @16:20:27.88 Elbow.reconfigure LooseElbow @16:20:27.93 Elbow.home.seek clear @16:20:32.61 SE.reconfigure SEconfig @16:20:32.65 SE.home.seek down @16:20:33.19 SC.reconfigure SCsloppy @16:20:33.24 SC.home.seek initial @16:20:35.45 Forearm.seek retract @16:20:35.52 Elbow.seek hammer @16:20:36.73 Forearm.reconfigure TightForearm @16:20:36.79 Forearm.seek 2 @16:20:37.94 Forearm.coast @16:20:37.99 Arm::Error in MAIN -- Holding position 2 requires -44mA @16:20:38.00 Retry #1 of 5 @16:20:38.01 Hammering Forearm against both stops to recenter bearing races @16:20:38.07 Forearm.force 100 @16:20:38.65 Forearm.coast @16:20:38.69 Forearm.force -100 @16:20:39.26 Forearm.coast @16:20:39.61 Forearm.seek 2 @16:20:39.89 Forearm.seek retract @16:20:40.90 Forearm.reconfigure LooseForearm @16:20:41.37PST23-Jan-14 ===> STARTED coreNegNTCWCR (phase #3) <=== @16:20:41.47 Starting mfb @16:20:41.48 pcrBAC wcr sampling at most 100ml @16:20:41.49 BACfans.email "Can@16:20:41, 21.5C, 52% humidity, 14.4psia, 13.724V, 0.707A, 0.695A avg, 9.71W\nCTD data unavailable\nISUS data unavailable",Subject => "pcrBAC wcr sampling at most 100ml" @16:20:43.35
Loading sh1 puck from tube 2 into Collection Clamp @16:20:43.46 Readying the MFB @16:20:43.47 Homing MFB Syringe @16:20:43.48 AFV.select :holdingCoil @16:20:43.53
Hand.release @16:20:43.66 ATV.dial :air2 @16:20:44.43
SC.reconfigure SCloose @16:20:44.58 Elbow.reconfigure TightElbow @16:20:44.64 Elbow.seek 2 @16:20:44.89 Forearm.reconfigure TightForearm @16:20:44.96 Forearm.seek 2 + 800 counts @16:20:44.98 AS.reconfigure ASconfig @16:20:45.06 AS.home.seek home @16:20:46.11
SE.reconfigure SEpick @16:20:46.18 SE.seek beamBreak @16:20:48.93 SE.configure SEconfig @16:20:49.09 Elbow.seek 2 @16:20:49.25 Forearm.seek 2 + 300 counts @16:20:49.49 Forearm.reconfigure LooseForearm @16:20:49.54 Hand.grip @16:20:50.28 SE.seek down @16:20:50.45
Forearm.seek retract @16:20:51.47 Elbow.seek CC @16:20:52.56 AFV.select :carrierSolution @16:20:52.66 delay 2 seconds @16:20:52.92 SE.coast @16:20:53.03
Elbow.reconfigure LooseElbow @16:20:53.12 Forearm.reconfigure TightForearm @16:20:53.17 Forearm.seek CC @16:20:54.33 CC.seek closed @16:20:54.67 AS.seek 1.000ml @16:20:58.11
Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.94 @16:20:58.12 Hand.release @16:20:58.93 Forearm.reconfigure LooseForearm @16:20:58.98 Forearm.seek retract @16:20:59.94 SC.coast @16:20:59.99 skipping prime and sample collection @16:21:00.00 Evacuating Puck (bubblePt=55.0psi, delay=15.0s) @16:21:00.01 Intake.select :close @16:21:00.09 CSV.dial :waste @16:21:00.13 CS.seek 0.000ml @16:21:00.26
Calibrating pressure and temperature sensors @16:21:00.28 CSV.dial :air @16:21:00.30
CTV.dial :air @16:21:00.33 SSV.dial :spit,avoiding => exhaust @16:21:00.36 SS.reconfigure SSfast @16:21:00.41 SS.seek 5.00ml @16:21:01.02 CS.seek 10.000ml @16:21:03.31 SSV.dial CBV @16:21:03.34
CBV.dial SSV @16:21:04.66 delay 3.8 seconds @16:21:08.54 SSV.dial :spit @16:21:09.85 SS.seek 0.00ml @16:21:12.57 SSV.dial CBV @16:21:13.88 SSV.dial CBV,avoiding => exhaust @16:21:13.89
CBV.dial SSV @16:21:13.90 CTV.dial :air @16:21:13.93 delay 15 seconds @16:21:28.94 SSV.dial :spit,avoiding => exhaust @16:21:30.25 CTV.dial CSV,avoiding => intake @16:21:30.27
SS.reconfigure SSexhale @16:21:30.35 SS.seek 0.00ml @16:21:30.46 SSV.dial CBV,avoiding => exhaust @16:21:32.12 CBV.dial between CSV and waste2b @16:21:33.08 CTV.dial CSV,avoiding => air @16:21:33.10
CSV.dial CTV @16:21:33.55 CS.reconfigure CSpressurize @16:21:33.60 CS.seek 0.000ml @16:21:46.45 delay 1 second @16:21:47.46 AFV.select :holdingCoil @16:21:47.56 delay 1 second @16:21:48.57 ATV.dial :waste @16:21:49.46 AS.reconfigure ASnorm @16:21:49.50 AS.home.seek empty @16:21:59.46
CSV.dial :air @16:21:59.99 CS.reconfigure CSfast @16:22:00.03 CS.seek 10.000ml @16:22:23.10 CSV.dial between air and CTV @16:22:23.71 CS.seek 3.257ml @16:22:39.69 CTV.dial CSV,avoiding => air @16:22:39.70
CSV.dial CTV @16:22:40.11 CS.reconfigure CSpressurize @16:22:40.16 CS.seek 0.000ml @16:22:42.79 ATV.dial :waste @16:22:42.80 ARV.dial between carrier and air1 @16:22:42.89 ASV.dial :waste1 @16:22:48.48
BubblePoint: Intake @69.5psia, Outlet @14.7psia @16:22:48.49 SS.reconfigure SSconfig @16:22:48.54 SS.seek 10.00ml @16:22:54.41 delay 15 seconds @16:23:09.51 Evacuated: Intake @68.5psia, Outlet @-0.3psia @16:23:09.52 SSV.dial :spit,avoiding => exhaust @16:23:10.84 SS.reconfigure SSexhale @16:23:10.88 SS.seek 0.00ml @16:23:15.74 SS.reconfigure SSfast @16:23:15.79 SS.seek 5.00ml @16:23:18.67 SSV.dial CBV,avoiding => exhaust @16:23:18.69
CBV.dial SSV @16:23:20.01 CTV.dial :air,avoiding => intake @16:23:21.30 CSV.dial CTV @16:23:21.32
SS.reconfigure SSconfig @16:23:21.38 SS.seek 10.00ml @16:23:22.32 CSV.dial :air @16:23:22.84 CS.reconfigure CSfast @16:23:22.89 CS.seek 0.000ml @16:23:24.15 CS.coast @16:23:24.41
SSV.dial :spit,avoiding => exhaust @16:23:25.72 SS.reconfigure SSexhale @16:23:25.77 SS.seek 0.00ml @16:23:30.94 SS.reconfigure SSfast @16:23:30.99 SS.seek 5.00ml @16:23:34.12 SSV.dial CBV,avoiding => exhaust @16:23:34.14
CBV.dial SSV @16:23:36.46 SSV.dial :spit,avoiding => exhaust @16:23:37.76 SS.reconfigure SSconfig @16:23:37.80 SS.seek 0.00ml @16:23:40.78 SS.coast @16:23:40.82 Evacuating Puck (bubblePt=55.0psi, delay=15.0s) @16:23:40.83 Intake.select :close @16:23:40.91 CSV.dial :waste @16:23:41.00
SSV.dial :exhaust @16:23:41.66 CS.seek 0.000ml @16:23:41.75
Exhaust.select :open @16:23:41.85 SS.reconfigure SSexhale @16:23:41.90 SS.seek 0.00ml @16:23:41.96 CSV.dial :air @16:23:42.38
Exhaust.select :close @16:23:42.48 SSV.dial CBV,avoiding => exhaust @16:23:42.50
CBV.dial SSV @16:23:43.17 CS.seek 10.000ml @16:23:44.36
CTV.dial :air @16:23:44.37 delay 15 seconds @16:23:59.38 SSV.dial :spit,avoiding => exhaust @16:24:00.70 CTV.dial CSV,avoiding => intake @16:24:00.72
SS.seek 0.00ml @16:24:00.83 SSV.dial CBV,avoiding => exhaust @16:24:06.51 CBV.dial between CSV and waste2b @16:24:07.44 CTV.dial CSV,avoiding => air @16:24:07.46
CSV.dial CTV @16:24:07.92 CS.reconfigure CSpressurize @16:24:07.96 CS.seek 0.000ml @16:24:33.83 CSV.dial :air @16:24:34.36 CS.reconfigure CSfast @16:24:34.40 CS.seek 10.000ml @16:24:57.59 CSV.dial between air and CTV @16:24:57.98 CS.seek 3.249ml @16:25:14.06 CTV.dial CSV,avoiding => air @16:25:14.07
CSV.dial CTV @16:25:14.39 CS.reconfigure CSpressurize @16:25:14.43 CS.seek 0.000ml @16:25:22.72 BubblePoint: Intake @69.6psia, Outlet @14.4psia @16:25:22.73 SS.reconfigure SSconfig @16:25:22.78 SS.seek 10.00ml @16:25:28.67 delay 15 seconds @16:25:43.77 Evacuated: Intake @68.8psia, Outlet @0.1psia SSV.dial :spit,avoiding => exhaust @16:25:45.08 SS.reconfigure SSexhale @16:25:45.13 SS.seek 0.00ml @16:25:50.64 SS.reconfigure SSfast @16:25:50.69 SS.seek 5.00ml @16:25:53.63 SSV.dial CBV,avoiding => exhaust @16:25:53.65
CBV.dial SSV @16:25:54.97 CTV.dial :air,avoiding => intake @16:25:56.27 CSV.dial CTV @16:25:56.29
SS.reconfigure SSconfig @16:25:56.34 SS.seek 10.00ml @16:25:57.28 CSV.dial :air @16:25:57.79 CS.reconfigure CSfast @16:25:57.84 CS.seek 0.000ml @16:25:59.11 CS.coast @16:25:59.54
SSV.dial :spit,avoiding => exhaust @16:26:00.85 SS.reconfigure SSexhale @16:26:00.90 SS.seek 0.00ml @16:26:06.03 SS.reconfigure SSfast @16:26:06.08 SS.seek 5.00ml @16:26:09.07 SSV.dial CBV,avoiding => exhaust @16:26:09.09
CBV.dial SSV @16:26:11.39 SSV.dial :spit,avoiding => exhaust @16:26:12.69 SS.reconfigure SSconfig @16:26:12.73 SS.seek 0.00ml @16:26:16.01 SS.coast @16:26:16.05 Lysing: Adding 1.5ml lysis @16:26:16.06 CBV.dial between CSV and waste1,avoiding => DBV @16:26:17.57 CTV.dial CSV @16:26:18.84 CSV.dial CSR @16:26:19.64 CSR.select :lysis @16:26:19.75 CS.reconfigure CSconfig @16:26:19.80 CS.seek 1.000ml @16:26:23.14 delay 5 seconds @16:26:28.15 CSR.select :air @16:26:28.31 CS.seek 6.000ml @16:26:41.79 delay 2 seconds @16:26:43.80 CSV.dial :waste1 @16:26:45.07 CS.reconfigure CSfast @16:26:45.11 CS.seek empty @16:26:59.29 CSV.dial CSR @16:27:00.56 CSR.select :lysis @16:27:00.67 CS.reconfigure CSconfig @16:27:00.72 CS.seek 1.500ml @16:27:05.35 delay 5 seconds @16:27:10.36 CSR.select :air @16:27:10.52 CS.seek 4.501ml @16:27:18.92 delay 2 seconds @16:27:20.93 CSV.dial CTV @16:27:21.46 CS.seek 1.275ml @16:27:30.43 Heating sh1 puck from tube 2 in Collection Clamp to 85.0C for 5:00 @16:27:30.44 CH.seek 85.0C,8:00 @16:29:44.21 CH.hold 5:00,85.0C @16:34:44.34 Collection Puck Heater at 85.0C after lysing @16:34:44.35 CS.seek 0.000ml @16:34:48.38 CTV.dial between spare and air,avoiding => intake @16:34:49.37 CSV.dial :air @16:34:49.82 CS.seek 5.000ml @16:35:03.34 CSV.dial CTV @16:35:03.86 delay 2 seconds @16:35:05.88 CSV.dial :air @16:35:06.39 delay 2 seconds @16:35:08.41 CSV.dial CTV @16:35:08.92 delay 2 seconds @16:35:10.94 CSV.dial :air @16:35:11.45 delay 2 seconds @16:35:13.46 CS.seek 0.000ml @16:35:26.87 Recovering Lysate into Collection syringe @16:35:26.88 CSV.dial CBV @16:35:27.89 CBV.dial CSV @16:35:28.25 CS.seek 5.001ml @16:35:41.72 delay 5 seconds @16:35:46.73 CTV.dial :air @16:35:47.22 CS.seek 7.001ml @16:35:53.15 delay 5 seconds @16:35:58.16 CSV.dial :air2 @16:35:58.60 CS.seek 10.000ml @16:36:07.09 CSV.dial between air and CTV,avoiding => CBV @16:36:08.22 Lysing Complete @16:36:08.28 Storing sh1 puck in Collection Clamp to tube 1 @16:36:08.42 SC.reconfigure SCtight @16:36:08.47 SC.seek 1 @16:36:10.75 SC.reconfigure SCloose @16:36:10.93 Elbow.reconfigure TightElbow @16:36:10.98 Elbow.seek 1 @16:36:12.56 Forearm.reconfigure TightForearm @16:36:12.61 Forearm.seek 1 + 800 counts @16:36:13.62 SE.reconfigure SEpick @16:36:13.69 SE.seek beamBreak @16:36:16.14 SE.configure SEconfig @16:36:16.27 Elbow.reconfigure LimpElbow @16:36:16.31 Forearm.reconfigure LooseForearm @16:36:16.36 Forearm.seek 1 @16:36:16.58 Elbow.hold @16:36:16.65 Elbow.reconfigure TightElbow @16:36:16.74 SE.seek 5.04 Pucks below Top Plate @16:36:17.10 Forearm.coast @16:36:17.13 Forearm.seek 1 + 337 counts @16:36:17.39 Forearm.reconfigure TightForearm @16:36:17.43 Forearm.seek 1 + 800 counts @16:36:17.72 SE.seek 6.04 Pucks below Top Plate @16:36:18.25 Forearm.reconfigure LooseForearm @16:36:18.30 Forearm.seek retract @16:36:19.23 Elbow.seek CC @16:36:20.76 Elbow.reconfigure LooseElbow @16:36:20.86 Forearm.seek CC @16:36:21.78 CC.seek sealed @16:36:22.71 Hand.grip @16:36:23.48 CC.seek open @16:36:26.84 Forearm.seek retract @16:36:27.82 Elbow.reconfigure TightElbow @16:36:27.87 Elbow.seek 1 @16:36:29.45 Elbow.reconfigure LimpElbow @16:36:29.51 Forearm.seek 1 @16:36:30.54 Elbow.hold @16:36:30.61 Elbow.reconfigure TightElbow @16:36:30.66 Hand.release @16:36:32.06 Forearm.coast @16:36:32.11 Forearm.seek 1 + 338 counts @16:36:32.36 Forearm.reconfigure TightForearm @16:36:32.41 Forearm.seek 1 + 800 counts @16:36:32.70 SE.seek down @16:36:33.80
Forearm.reconfigure LooseForearm @16:36:33.85 Forearm.seek retract @16:36:34.87 SE.coast @16:36:34.91
SC.coast @16:36:34.95 Tranferring lysate to PRV @16:36:34.96 CSV.dial :air @16:36:35.49 CS.seek 10.000ml @16:36:35.56 CSV.dial between air and CTV @16:36:35.86 AIV.dial :bypass @16:36:35.94 Beginning samplePassC @16:36:35.95 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:36:36.11 Elbow.seek FlushPuck::Garage @16:36:37.87 Elbow.reconfigure LooseElbow @16:36:37.97 Forearm.seek garage @16:36:38.96 Hand.grip @16:36:39.82 Forearm.seek retract @16:36:40.87 Elbow.reconfigure TightElbow @16:36:40.91 Elbow.seek CC @16:36:43.70 Elbow.reconfigure LooseElbow @16:36:43.79 Forearm.reconfigure TightForearm @16:36:43.83 Forearm.seek CC @16:36:44.93 CC.seek closed @16:36:48.70 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.92 @16:36:48.76 Hand.release @16:36:49.52 Forearm.reconfigure LooseForearm @16:36:49.57 Forearm.seek retract @16:36:50.55 AWV.select :air @16:36:50.56 AIV.dial :bypass @16:36:50.58 PSV.dial PSR @16:36:51.90 PSR.select :flush @16:36:52.01 PS.seek 1.500ml @16:37:08.06 delay 5 seconds @16:37:13.07 PSV.dial :waste1 @16:37:13.55 PS.seek 0.000ml @16:37:29.65 PSV.dial PSR @16:37:30.10 PSR.select :air @16:37:30.22 PS.seek 2.500ml @16:37:56.50 CBV.dial PRV @16:37:56.51 PRV.dial CBV @16:37:57.27
PSV.dial :mixloop @16:37:58.29 CTV.dial :air,avoiding => intake @16:37:58.30 PTV.dial :PRVmixing @16:37:58.39 PS.seek 0.000ml @16:38:24.55 PSV.dial :air @16:38:25.02 PS.seek 2.500ml @16:38:51.30 PSV.dial :mixloop @16:38:51.76 PS.seek 0.000ml @16:39:17.93 PSV.dial :air @16:39:18.39 PS.seek 2.500ml @16:39:44.67 PSV.dial :mixloop @16:39:45.14 PS.seek 0.000ml @16:40:11.39 Garaging FlushPuck in Collection Clamp @16:40:11.46 Elbow.reconfigure TightElbow @16:40:11.51 Elbow.seek CC @16:40:11.61 Elbow.reconfigure LooseElbow @16:40:11.71 Forearm.seek CC @16:40:12.69 CC.seek sealed @16:40:13.61 Hand.grip @16:40:14.35 CC.seek open @16:40:17.67 Forearm.seek retract @16:40:18.67 Elbow.reconfigure TightElbow @16:40:18.72 Elbow.seek garage @16:40:21.51 Elbow.reconfigure LooseElbow @16:40:21.60 Forearm.reconfigure TightForearm @16:40:22.21 Forearm.seek garage @16:40:23.40 Hand.release @16:40:24.14 Forearm.reconfigure LooseForearm @16:40:24.19 Forearm.seek retract @16:40:25.31 Connecting PS to CS to transfer sample @16:40:25.32 CBV.dial AIV @16:40:25.33 AIV.dial :bypass @16:40:25.34 PSV.dial :mixloop @16:40:25.35 PTV.dial :PRVmixing @16:40:25.37 PRV.dial AIV @16:40:25.38 CTV.dial :air,avoiding => intake @16:40:25.40 Loading lyfil puck from tube 2 into Collection Clamp @16:40:25.63 SC.reconfigure SCtight @16:40:25.68 SC.seek 2 @16:40:27.95 SC.reconfigure SCloose @16:40:28.13 Elbow.reconfigure TightElbow @16:40:28.18 Elbow.seek 2 @16:40:30.00 Forearm.reconfigure TightForearm @16:40:30.14 Forearm.seek 2 + 800 counts @16:40:31.23 SE.reconfigure SEpick @16:40:31.30 SE.seek beamBreak @16:40:34.19 SE.configure SEconfig @16:40:34.32 Elbow.seek 2 @16:40:34.47 Forearm.seek 2 + 300 counts @16:40:34.70 Forearm.reconfigure LooseForearm @16:40:34.75 Hand.grip @16:40:35.48 SE.seek down @16:40:35.63
Forearm.seek retract @16:40:36.61 Elbow.seek CC @16:40:38.15 Elbow.reconfigure LooseElbow @16:40:38.21 SE.coast @16:40:38.30
Forearm.reconfigure TightForearm @16:40:38.34 Forearm.seek CC @16:40:39.42 CC.seek closed @16:40:43.19 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.96 @16:40:43.20 Hand.release @16:40:44.01 Forearm.reconfigure LooseForearm @16:40:44.06 Forearm.seek retract @16:40:45.01 SC.coast @16:40:45.05 CTV.dial CSV,avoiding => intake @16:40:46.36 CSV.dial CTV @16:40:46.66 CS.seek 5.000ml @16:41:00.11 delay 1:20 @16:42:20.12 CS.seek 2.500ml @16:42:27.29 delay 40 seconds @16:43:07.30 CS.seek 0.000ml @16:43:14.51 delay 1:00 @16:44:14.52 PSV.dial :air @16:44:14.97 PS.seek 2.500ml @16:44:41.29 PSV.dial :mixloop @16:44:41.74 delay 3 seconds @16:44:44.75 PSV.dial :air @16:44:45.32 delay 2 seconds @16:44:47.33 PSV.dial :mixloop @16:44:47.76 delay 3 seconds @16:44:50.77 PSV.dial :waste1 @16:44:52.06 PS.seek empty @16:45:18.32 CBV.dial :waste1 @16:45:18.78 delay 5 seconds @16:45:23.79 CBV.dial AIV @16:45:24.25 delay 10 seconds @16:45:34.26 CBV.dial :waste1 @16:45:34.73 delay 10 seconds @16:45:44.74 CBV.dial AIV @16:45:45.92 delay 10 seconds @16:45:55.93 CTV.dial CSV,avoiding => intake @16:45:55.94 CBV.dial between AIV and waste1 @16:45:56.30 CSV.dial :air @16:45:56.82 CS.seek 10.000ml @16:46:23.00 CSV.dial CTV @16:46:23.52 CS.seek 0.000ml @16:46:49.62 CBV.dial :waste1 @16:46:49.93 delay 10 seconds @16:46:59.94 CTV.dial :air,avoiding => intake @16:47:01.28 Storing lyfil puck in Collection Clamp to tube 1 @16:47:01.42 SC.reconfigure SCtight @16:47:01.46 SC.seek 1 @16:47:03.75 SC.reconfigure SCloose @16:47:03.93 Elbow.reconfigure TightElbow @16:47:03.97 Elbow.seek 1 @16:47:05.61 Forearm.reconfigure TightForearm @16:47:05.66 Forearm.seek 1 + 800 counts @16:47:06.84 SE.reconfigure SEpick @16:47:06.90 SE.seek beamBreak @16:47:09.82 SE.configure SEconfig @16:47:09.96 Elbow.reconfigure LimpElbow @16:47:10.01 Forearm.reconfigure LooseForearm @16:47:10.06 Forearm.seek 1 @16:47:10.29 Elbow.hold @16:47:10.35 Elbow.reconfigure TightElbow @16:47:10.44 SE.seek 6.04 Pucks below Top Plate @16:47:10.79 Forearm.coast @16:47:10.82 Forearm.seek 1 + 349 counts @16:47:11.08 Forearm.reconfigure TightForearm @16:47:11.12 Forearm.seek 1 + 800 counts @16:47:11.41 SE.seek 7.04 Pucks below Top Plate @16:47:11.96 Forearm.reconfigure LooseForearm @16:47:12.01 Forearm.seek retract @16:47:12.94 Elbow.seek CC @16:47:14.52 Elbow.reconfigure LooseElbow @16:47:14.62 Forearm.seek CC @16:47:15.58 CC.seek sealed @16:47:16.50 Hand.grip @16:47:17.20 CC.seek open @16:47:20.59 Forearm.seek retract @16:47:21.55 Elbow.reconfigure TightElbow @16:47:21.61 Elbow.seek 1 @16:47:23.18 Elbow.reconfigure LimpElbow @16:47:23.23 Forearm.seek 1 @16:47:24.29 Elbow.hold @16:47:24.35 Elbow.reconfigure TightElbow @16:47:24.40 Hand.release @16:47:25.28 Forearm.coast @16:47:25.33 Forearm.seek 1 + 325 counts @16:47:25.58 Forearm.reconfigure TightForearm @16:47:25.63 Forearm.seek 1 + 800 counts @16:47:25.93 SE.seek down @16:47:27.04
Forearm.reconfigure LooseForearm @16:47:27.09 Forearm.seek retract @16:47:28.00 SE.coast @16:47:28.06
SC.coast @16:47:28.09 PSV.dial :air @16:47:29.16 PS.seek 0.499ml @16:47:35.03 delay 3 seconds @16:47:38.04 PSV.dial :mixloop @16:47:38.48 PRV.dial :air @16:47:38.92 delay 3 seconds @16:47:41.93 PRV.dial CBV @16:47:42.37 CBV.dial AIV @16:47:42.84 PRV.dial between CBV and air @16:47:43.16 PRV.dial :air @16:47:43.52 PS.reconfigure PSslow @16:47:43.56 PS.seek 0.599ml @16:47:46.09 PRV.dial :waste2 @16:47:46.82 PS.reconfigure PSconfig @16:47:46.86 PS.seek empty @16:47:53.81 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:47:53.97 Elbow.seek FlushPuck::Garage @16:47:55.75 Elbow.reconfigure LooseElbow @16:47:55.85 Forearm.seek garage @16:47:56.85 Hand.grip @16:47:57.66 Forearm.seek retract @16:47:58.71 Elbow.reconfigure TightElbow @16:47:58.76 Elbow.seek CC @16:48:01.53 Elbow.reconfigure LooseElbow @16:48:01.63 Forearm.reconfigure TightForearm @16:48:01.67 Forearm.seek CC @16:48:02.74 CC.seek closed @16:48:06.52 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 2.00 @16:48:06.59 Hand.release @16:48:07.37 Forearm.reconfigure LooseForearm @16:48:07.95 Forearm.seek retract @16:48:09.05 Clearing PML for modified lysate @16:48:09.06 CTV.dial :air,avoiding => intake @16:48:09.08 PRV.dial :waste2 @16:48:09.10 PTV.dial :PRV,avoiding => puck @16:48:09.84 delay 2 seconds @16:48:11.84 PTV.dial :PRVmixing,avoiding => puck @16:48:12.58 delay 2 seconds @16:48:14.59 PSV.dial :PML @16:48:14.60 delay 2 seconds @16:48:16.62 PRV.dial :air @16:48:17.37 delay 2 seconds @16:48:19.43 PS.seek 1.499ml @16:48:35.50 delay 5 seconds @16:48:40.51 PS.seek 2.500ml @16:48:51.46 PSV.dial :air @16:48:51.93 PS.seek 0.000ml @16:49:18.09 mixing lysate with speDil @16:49:18.10 PSV.dial :PML @16:49:18.57 CBV.dial :PRV @16:49:18.58 PRV.dial :air @16:49:18.60 delay 2 seconds @16:49:20.60 PRV.dial :speDil @16:49:21.06 delay 2 seconds @16:49:23.13 PS.reconfigure PSslow1 @16:49:23.18 PS.seek 0.225ml @16:49:46.40 delay 5 seconds @16:49:51.41 PRV.dial CBV @16:49:52.17 delay 2 seconds @16:49:54.23 PS.seek 0.475ml @16:50:19.93 delay 5 seconds @16:50:24.94 PRV.dial :air @16:50:25.40 delay 2 seconds @16:50:27.41 PS.seek 1.000ml @16:51:21.09 delay 10 seconds @16:51:31.10 PRV.dial CBV @16:51:31.56 CTV.dial :air,avoiding => intake @16:51:31.57 PSV.dial :air @16:51:32.06 PS.reconfigure PSconfig @16:51:32.10 PS.seek 2.500ml @16:51:48.09 delay 3 seconds @16:51:51.11 PSV.dial PTV @16:51:51.86 PTV.dial :PRV,avoiding => puck @16:51:52.60 PS.seek 0.000ml @16:52:18.87 PSV.dial :air @16:52:19.63 PS.seek 2.500ml @16:52:45.93 delay 3 seconds @16:52:48.94 PSV.dial PTV @16:52:49.79 PTV.dial :PRV,avoiding => puck @16:52:49.81 PS.seek 0.000ml @16:53:16.06 Pushing modified lysate into AIV Injection Loop @16:53:16.07 CBV.dial AIV @16:53:16.08 AIV.dial :bypass @16:53:16.10 PRV.dial CBV @16:53:16.12 PSV.dial :air @16:53:16.97 PS.seek 2.500ml @16:53:43.15 delay 3 seconds @16:53:46.16 PSV.dial :PML @16:53:46.60 PTV.dial :PRVmixing,avoiding => puck @16:53:47.39 PS.reconfigure PSslow1 @16:53:47.43 PS.seek 1.740ml @16:55:05.05 delay 5 seconds @16:55:10.06 AIV.dial :sample @16:55:10.61 AWV.select :waste @16:55:10.77 PS.reconfigure PSslow2 @16:55:10.81 PS.seek 1.220ml @16:56:56.92 delay 15 seconds @16:57:11.93 AIV.dial :bypass @16:57:12.50 PS.reconfigure PSconfig @16:57:12.54 PS.seek 0.000ml @16:57:25.70 PSV.dial :air @16:57:26.17 PS.seek 2.500ml @16:57:52.48 PSV.dial :PML @16:57:52.95 PS.seek 0.000ml @16:58:19.23 AIV.dial :coilToMFB @16:58:19.24 AWV.select :air @16:58:19.40 Extracting Nucleic Acid from AIV @16:58:19.42
Beginning samplePassD @16:58:19.45 SPE.hold 2:46:40,55.0C @16:58:19.48 ASV.dial :SPEdelivery @16:58:19.60
AWV.select :air @16:58:19.61 AIV.dial :bypass @16:58:19.62 PSV.dial PSR @16:58:20.33 delay 20 seconds @16:58:20.69
PSR.select :killMFB @16:58:20.80 PS.seek 1.500ml @16:58:37.01 delay 5 seconds @16:58:40.34 primeLysateINT @16:58:40.44 AWV.select :air @16:58:40.45 AIV.dial :coilToMFB @16:58:40.47 ATV.dial :AIV @16:58:41.81 AS.reconfigure ASslow1 @16:58:41.86 AS.seek 0.241ml @16:58:42.02
PSV.dial :waste1 @16:58:42.49 PS.seek 0.000ml @16:58:58.61 PSV.dial PSR @16:58:59.09 PSR.select :air @16:58:59.21 PS.seek 2.500ml @16:59:25.45 PRV.dial CBV @16:59:25.47
CBV.dial PRV @16:59:25.49 PSV.dial :mixloop @16:59:26.57 CTV.dial :air,avoiding => intake @16:59:26.58 PTV.dial :PRVmixing @16:59:26.60 PS.seek 0.000ml @16:59:31.50 delay 10 seconds @16:59:41.51 ATV.dial :air2 @16:59:42.60 AS.seek 0.271ml @16:59:48.98 ATV.dial :waste2 @16:59:49.35 AS.reconfigure ASnorm @16:59:49.40 AS.seek empty @16:59:52.92
PSV.dial PSR @16:59:53.99 PSR.select :flush @16:59:54.10 PS.seek 1.500ml @17:00:03.67 Lysate primed! @17:00:03.68 AFV.select :holdingCoil @17:00:03.69 ATV.dial :air2 @17:00:04.11 AS.reconfigure ASslow1 @17:00:04.16 AS.seek 0.069ml @17:00:10.23
delay 5 seconds @17:00:15.24 PSV.dial :waste1 @17:00:15.72 PS.seek 0.000ml @17:00:18.76 delay 3 seconds @17:00:21.77 ATV.dial :AIV @17:00:22.34 AS.seek 0.469ml @17:00:31.98
PSV.dial PSR @17:00:32.46 PSR.select :air @17:00:32.57 PS.seek 2.500ml @17:00:58.79 PRV.dial CBV @17:00:58.81
CBV.dial PRV @17:00:58.83 PSV.dial :mixloop @17:00:59.90 CTV.dial :air,avoiding => intake @17:00:59.91 PTV.dial :PRVmixing @17:00:59.94 PS.seek 0.000ml @17:01:26.16 PSV.dial PSR @17:01:27.21 PSR.select :flush @17:01:27.32 PS.seek 1.500ml @17:01:43.41 delay 5 seconds @17:01:44.15 delay 5 seconds @17:01:48.42
PSV.dial :waste1 @17:01:48.89 PS.seek 0.000ml @17:01:49.20 Solid Phase Extraction Heater at 53.6C @17:01:49.21 ATV.dial ASV @17:01:49.55 ASV.dial :SPEdelivery @17:01:49.63 AS.seek 0.041ml @17:02:04.94
PSV.dial PSR @17:02:05.41 PSR.select :air @17:02:05.52 PS.seek 2.500ml @17:02:31.79 PRV.dial CBV @17:02:31.81
CBV.dial PRV @17:02:31.83 PSV.dial :mixloop @17:02:32.88 CTV.dial :air,avoiding => intake @17:02:32.89 PTV.dial :PRVmixing @17:02:32.92 PS.seek 0.000ml @17:02:59.19 PSV.dial PSR @17:03:00.22 PSR.select :flush @17:03:00.33 PS.seek 1.500ml @17:03:16.44 delay 5 seconds @17:03:17.19 delay 10 seconds @17:03:21.45
PSV.dial :waste1 @17:03:21.92 PS.seek 0.000ml @17:03:27.20 rinseLysateLineINT @17:03:27.21 AFV.select :carrierSolution @17:03:27.31 delay 2 seconds @17:03:29.32 AS.reconfigure ASconfig @17:03:29.37 AS.seek 1.000ml @17:03:38.00
PSV.dial PSR @17:03:38.47 PSR.select :air @17:03:38.58 PS.seek 2.500ml @17:04:04.91 PRV.dial CBV @17:04:04.93
CBV.dial PRV @17:04:04.95 PSV.dial :mixloop @17:04:05.97 CTV.dial :air,avoiding => intake @17:04:05.98 PTV.dial :PRVmixing @17:04:06.00 PS.reconfigure PSslow @17:04:06.06 PS.seek 1.200ml @17:04:25.59 delay 1 second @17:04:26.60 AFV.select :holdingCoil @17:04:26.70 delay 1 second @17:04:27.71 ATV.dial :AIV @17:04:28.16 AIV.dial :coilToMFB @17:04:28.17 AWV.select :waste @17:04:28.27 AS.reconfigure ASslow0 @17:04:28.32 AS.seek 0.000ml @17:04:32.94
PSV.dial :waste1 @17:04:34.27 PS.reconfigure PSconfig @17:04:34.31 PS.seek 0.000ml @17:04:47.53 PSV.dial :air @17:04:48.59 PS.seek 2.500ml @17:05:14.81 PSV.dial :mixloop @17:05:15.27 PS.reconfigure PSslow @17:05:15.31 PS.seek 0.000ml @17:06:06.65 PSV.dial :air @17:06:07.13 PS.reconfigure PSconfig @17:06:07.17 PS.seek 2.500ml @17:06:10.64 ATV.dial :air2 @17:06:11.22 AS.reconfigure ASslow1 @17:06:11.27 AS.seek 0.050ml @17:06:21.76 ATV.dial :AIV @17:06:22.33 AS.seek 0.010ml @17:06:30.72 ATV.dial :waste @17:06:31.99 AS.seek empty @17:06:33.40
PSV.dial :mixloop @17:06:33.86 PS.reconfigure PSslow @17:06:33.91 PS.seek 0.000ml @17:06:34.42 ethanol wash @17:06:34.43 ATV.dial :air2 @17:06:35.38 AS.reconfigure ASslow2 @17:06:35.43 AS.seek 0.009ml @17:06:45.95 delay 3 seconds @17:06:48.96 Pulling 0.4ml of Ethanol Wash @17:06:48.97 ATV.dial :ethanol @17:06:49.55 AS.reconfigure ASslow1 @17:06:49.60 AS.seek 0.409ml @17:07:25.27
Cleaning Collection Syringe @17:07:25.28 CSV.dial CSR @17:07:25.73 CSR.select :flush @17:07:26.35 CS.seek 4.000ml @17:07:37.26 delay 5 seconds @17:07:42.27 CSR.select :air @17:07:42.38 CS.seek 10.000ml @17:07:58.38 CSV.dial CTV @17:07:58.42
CTV.dial CSV @17:07:59.74 CBV.dial :waste1 @17:08:00.24 CS.seek 0.000ml @17:08:11.45 delay 3 seconds @17:08:14.46 ATV.dial :air1 @17:08:14.79 delay 3 seconds @17:08:17.85 AS.seek 0.434ml @17:08:23.24 delay 3 seconds @17:08:26.25 ATV.dial :ethanol @17:08:26.36
CSV.dial CSR @17:08:26.63 AS.seek 0.419ml @17:08:26.88
CSR.select :flush @17:08:26.99 CS.seek 4.000ml @17:08:29.94 delay 3 seconds @17:08:32.95 ATV.dial ASV @17:08:34.07 AS.seek 0.092ml @17:08:37.92
delay 5 seconds @17:08:42.93 CSR.select :air @17:08:43.04 CS.seek 10.000ml @17:08:59.14 CSV.dial CTV @17:08:59.16
CTV.dial CSV @17:08:59.70 CBV.dial :waste1 @17:08:59.71 CS.seek 0.000ml @17:09:25.91 Garaging FlushPuck in Collection Clamp @17:09:25.98 Elbow.reconfigure TightElbow @17:09:26.02 Elbow.seek CC @17:09:26.14 Elbow.reconfigure LooseElbow @17:09:26.23 Forearm.seek CC @17:09:27.18 CC.seek sealed @17:09:28.14 Hand.grip @17:09:28.91 CC.seek open @17:09:32.23 Forearm.seek retract @17:09:33.17 Elbow.reconfigure TightElbow @17:09:33.22 Elbow.seek garage @17:09:36.03 Elbow.reconfigure LooseElbow @17:09:36.12 Forearm.reconfigure TightForearm @17:09:36.16 Forearm.seek garage @17:09:37.32 Hand.release @17:09:38.08 Forearm.reconfigure LooseForearm @17:09:38.13 Forearm.seek retract @17:09:39.17 Whole Cell Archive sampling 100ml @17:09:39.19 Loading wcr puck from tube 2 into Collection Clamp @17:09:39.39 SC.reconfigure SCtight @17:09:39.44 SC.seek 2 @17:09:40.99 delay 3 seconds @17:09:41.80 SC.reconfigure SCloose @17:09:41.99 Elbow.reconfigure TightElbow @17:09:42.04 Elbow.seek 2 @17:09:43.94 Forearm.reconfigure TightForearm @17:09:43.99 Forearm.seek 2 + 800 counts @17:09:44.03 ATV.dial :waste2 @17:09:44.57 AS.reconfigure ASconfig @17:09:44.61 AS.seek empty @17:09:45.12 SE.reconfigure SEpick @17:09:45.19 SE.seek beamBreak @17:09:48.12 SE.configure SEconfig @17:09:48.26 Elbow.seek 2 @17:09:48.58 Forearm.seek 2 + 300 counts @17:09:48.79 Forearm.reconfigure LooseForearm @17:09:48.84 Hand.grip @17:09:49.55 SE.seek down @17:09:49.70 Forearm.seek retract @17:09:50.31 ATV.dial :air2 @17:09:50.67 AS.reconfigure ASslow1 @17:09:50.72 AS.seek 1.000ml @17:09:50.79 Elbow.seek CC @17:09:52.34 SE.coast @17:09:52.39 Elbow.reconfigure LooseElbow @17:09:52.49 Forearm.reconfigure TightForearm @17:09:52.54 Forearm.seek CC @17:09:53.67 CC.seek closed @17:09:58.11 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.97 @17:09:58.12 Hand.release @17:09:58.95 Forearm.reconfigure LooseForearm @17:09:59.00 Forearm.seek retract @17:09:59.95 SC.coast @17:09:59.99 collectSample 100ml w/bubble point of 55psi @17:10:00.01 Priming 7ml @17:10:00.02 Intake.select :close @17:10:00.04 Exhaust.select :close @17:10:00.06 SS.hold @17:10:00.25 Calibrating pressure sensors @17:10:00.26 CTV.dial :air @17:10:01.56 SSV.dial :spit,avoiding => exhaust @17:10:01.57 SS.reconfigure SSfast @17:10:01.63 SS.seek 5.00ml @17:10:04.37 SSV.dial CBV @17:10:04.40 CBV.dial SSV @17:10:05.96 delay 3.8 seconds @17:10:09.86 SSV.dial :spit @17:10:11.17 SS.seek 0.00ml @17:10:13.90 SSV.dial CBV @17:10:15.23 CTV.dial :intake @17:10:15.98 SSV.dial CBV @17:10:15.99 CBV.dial SSV @17:10:16.01 Allowing 55s for 7.0ml of remaining 7.0ml @17:10:16.03 Intake.select :open @17:10:18.19 Intake @19.7psia, vacuum from 13.2 to 17.2psi @17:10:18.21 SS.reconfigure forSampling @17:10:18.27 SS.seek 7.00ml @17:10:24.05 Stroke completed -- waiting for pressure to equalize ... @17:11:04.62 SS.reconfigure SSconfig @17:11:04.67 SS.hold @17:11:04.78 Intake.select :close @17:11:04.94 ShallowSampler::Clogged in WCR -- Intake @20psia & Outlet @7psia won't equalize after 7.0ml @17:11:04.95 SSV.dial :exhaust @17:11:05.72 Exhaust.select :open @17:11:05.83 SS.reconfigure SSexhale @17:11:05.88 SS.seek 0.00ml @17:11:09.54 Exhaust.select :close @17:11:09.64 Sampling 100.0ml @17:11:09.65 Intake.select :close @17:11:09.66 Exhaust.select :close @17:11:09.68 SS.reconfigure SSconfig @17:11:09.73 SS.hold @17:11:09.90 CTV.dial :intake @17:11:09.92 SSV.dial CBV @17:11:09.94 CBV.dial SSV @17:11:10.73 Allowing 185s for 25.0ml of remaining 100.0ml @17:11:10.74 Intake.select :open @17:11:12.89 Intake @20.3psia, vacuum from 13.8 to 17.8psi @17:11:12.91 SS.reconfigure forSampling @17:11:13.03 SS.seek 25.00ml @17:11:16.56 Vacuum too high -- waiting up to 40s @17:11:33.48 Intake @20.2psia, vacuum from 13.7 to 17.7psi @17:11:33.50 SS.reconfigure forSampling @17:11:33.56 SS.seek 25.00ml @17:11:36.35 Vacuum too high -- waiting up to 40s @17:11:54.96 SS.reconfigure forSampling @17:11:55.01 SS.seek 25.00ml @17:11:58.16 Vacuum too high -- waiting up to 40s @17:12:16.98 SS.reconfigure forSampling @17:12:17.03 SS.seek 25.00ml @17:12:20.40 Vacuum too high -- waiting up to 40s @17:12:41.14 Intake @20.2psia, vacuum from 13.7 to 17.7psi @17:12:41.15 SS.reconfigure forSampling @17:12:41.20 SS.seek 25.00ml @17:12:45.29 Vacuum too high -- waiting up to 40s @17:13:06.82 Intake @20.3psia, vacuum from 13.8 to 17.8psi @17:13:07.62 SS.reconfigure forSampling @17:13:07.68 SS.seek 25.00ml @17:13:12.04 Stroke completed -- waiting for pressure to equalize ... @17:13:15.17 delay 5 seconds @17:13:20.18 ATV.dial ASV @17:13:20.91 ASV.dial :SPEdelivery @17:13:20.93 AS.seek 0.020ml @17:13:49.67 Intake.select :close @17:13:49.81 SSV.dial :exhaust @17:13:50.57 Exhaust.select :open @17:13:50.67 SS.reconfigure SSexhale @17:13:50.72 SS.seek 0.00ml @17:13:59.51 Exhaust.select :close @17:13:59.62 SSV.dial CBV @17:13:59.64 CBV.dial SSV @17:14:00.42 Allowing 185s for 25.0ml of remaining 75.0ml @17:14:00.44 Intake.select :open @17:14:02.59 Intake @20.9psia, vacuum from 14.4 to 18.4psi @17:14:02.61 SS.reconfigure forSampling @17:14:02.67 SS.seek 25.00ml @17:14:05.31 Vacuum too high -- waiting up to 40s @17:14:09.30 Intake @20.3psia, vacuum from 13.8 to 17.8psi @17:14:09.32 SS.reconfigure forSampling @17:14:09.37 SS.seek 25.00ml @17:14:11.09 Vacuum too high -- waiting up to 40s @17:14:15.77 Intake @20.4psia, vacuum from 13.9 to 17.9psi @17:14:15.79 SS.reconfigure forSampling @17:14:15.85 SS.seek 25.00ml @17:14:18.25 Vacuum too high -- waiting up to 40s @17:14:22.83 Intake @20.3psia, vacuum from 13.8 to 17.8psi @17:14:22.84 SS.reconfigure forSampling @17:14:22.89 SS.seek 25.00ml @17:14:27.46 Vacuum too high -- waiting up to 40s @17:14:32.62 Intake @20.4psia, vacuum from 13.9 to 17.9psi @17:14:32.63 SS.reconfigure forSampling @17:14:32.69 SS.seek 25.00ml @17:15:25.92 Vacuum too high -- waiting up to 40s @17:15:34.34 SS.seek 25.00ml @17:16:05.15 Stroke completed -- waiting for pressure to equalize ... @17:16:23.98 Intake.select :close @17:16:24.12 SSV.dial :exhaust @17:16:24.89 Exhaust.select :open @17:16:24.99 SS.reconfigure SSexhale @17:16:25.04 SS.seek 0.00ml @17:16:34.00 Exhaust.select :close @17:16:34.10 SSV.dial CBV @17:16:34.12 CBV.dial SSV @17:16:34.89 Allowing 185s for 25.0ml of remaining 50.0ml @17:16:34.91 Intake.select :open @17:16:37.06 Intake @20.7psia, vacuum from 14.2 to 18.2psi @17:16:37.08 SS.reconfigure forSampling @17:16:37.14 SS.seek 25.00ml @17:16:41.14 delay 5 seconds @17:16:46.15 ATV.dial :air2 @17:16:47.01 AS.seek 0.520ml @17:17:28.45 Vacuum too high -- waiting up to 40s @17:17:35.93 Intake @20.4psia, vacuum from 13.9 to 17.9psi @17:17:35.95 SS.seek 25.00ml @17:18:29.25 delay 5 seconds @17:18:34.26 ATV.dial ASV @17:18:34.95 ASV.dial :wasteR @17:18:35.20 Stroke completed -- waiting for pressure to equalize ... @17:18:35.45 AS.seek 0.300ml @17:18:59.27 Intake.select :close @17:18:59.42 SSV.dial :exhaust @17:19:00.20 Exhaust.select :open @17:19:00.31 SS.reconfigure SSexhale @17:19:00.36 SS.seek 0.00ml @17:19:09.07 Exhaust.select :close @17:19:09.18 SSV.dial CBV @17:19:09.20 CBV.dial SSV @17:19:10.04 Allowing 185s for 25.0ml of remaining 25.0ml @17:19:10.06 Intake.select :open @17:19:12.21 Intake @20.9psia, vacuum from 14.4 to 18.4psi @17:19:12.23 SS.reconfigure forSampling @17:19:12.29 SS.seek 25.00ml @17:19:20.58 delay 20 seconds @17:19:40.59 AS.seek 0.070ml @17:19:43.08 Vacuum too high -- waiting up to 40s @17:19:48.06 Intake @20.5psia, vacuum from 14.0 to 18.0psi @17:19:48.07 SS.seek 25.00ml @17:20:27.76 delay 20 seconds @17:20:34.94 Vacuum too high -- waiting up to 40s @17:20:45.06 Intake @20.6psia, vacuum from 14.1 to 18.1psi @17:20:45.08 SS.seek 25.00ml @17:20:47.77 ATV.dial :waste2 @17:20:48.30 AS.reconfigure ASconfig @17:20:48.34 AS.seek empty @17:20:52.76 Elution @17:20:52.77 ATV.dial :air1 @17:20:53.72 AS.reconfigure ASslow1 @17:20:53.77 AS.seek 0.049ml @17:21:04.27 delay 3 seconds @17:21:07.28 ATV.dial :ElutionWater @17:21:07.84 AS.seek 0.109ml @17:21:18.63 Stroke completed -- waiting for pressure to equalize ... @17:21:20.42 delay 3 seconds @17:21:23.43 ATV.dial ASV,via => air2 @17:21:25.18 ASV.dial :SPEelution @17:21:25.85 AFV.select :carrierSolution @17:21:26.01 AS.reconfigure ASnorm @17:21:26.05 AS.seek 0.759ml @17:21:39.25 Intake.select :close @17:21:39.40 SSV.dial :exhaust @17:21:40.17 Exhaust.select :open @17:21:40.99 SS.reconfigure SSexhale @17:21:41.04 SS.seek 0.00ml @17:21:49.95 Exhaust.select :close @17:21:50.06 Sampled 100.0ml @17:21:50.07 collectSample: Complete @17:21:50.09 Drying puck before fixing for 04:00.00 @17:21:50.11 CBV.dial :waste2b @17:21:50.88 delay 1:00 @17:21:59.59 delay 3 seconds @17:22:02.60 AFV.select :holdingCoil @17:22:02.75 AS.reconfigure ASslow1 @17:22:02.79 AS.seek 0.489ml @17:22:50.88 CSV.dial :air @17:22:51.33 CS.reconfigure CSfast @17:22:51.38 CS.seek 10.000ml @17:22:58.13 delay 5 seconds @17:23:03.19 AS.reconfigure ASslow2 @17:23:03.24 AS.seek 0.454ml @17:23:14.63 CBV.dial between CSV and waste2b @17:23:14.96 Pumping wcr puck from tube 2 up to 55psi... @17:23:15.02 CBV.dial SSV @17:23:16.47 CBV.dial between CSV and waste2b @17:23:17.43 CSV.dial between air and CTV @17:23:18.63 CS.seek 6.860ml @17:23:26.64 CTV.dial CSV,avoiding => air @17:23:26.66 CSV.dial CTV @17:23:27.47 CS.reconfigure CSpressurize @17:23:27.52 CS.seek 0.000ml @17:23:39.13 delay 1:00 @17:23:41.53 CBV.dial :waste2b @17:23:41.89 CS.reconfigure CSconfig @17:23:41.94 CS.seek empty @17:23:46.44 delay 1:00 @17:24:39.14 pushing eluate to holding coil @17:24:39.20 AS.seek 0.294ml @17:24:46.45 CTV.dial :air,avoiding => intake @17:24:47.75 Fix #1 of 2 (with rnal) for 02:00.00 @17:24:47.76 CS Pulling 2ml of rnal @17:24:47.78 CSV.dial CSR @17:24:48.71 CSR.select :rnal @17:24:48.82 CS.seek 2.000ml @17:24:54.72 delay 5 seconds @17:24:59.73 CSR.select :air @17:24:59.84 CS.reconfigure CSfast @17:24:59.89 CS.seek 10.000ml @17:25:18.78 CBV.dial between CSV and waste2b @17:25:19.12 Pumping wcr puck from tube 2 up to 55psi... @17:25:19.18 CBV.dial SSV @17:25:20.62 CBV.dial between CSV and waste2b @17:25:21.59 CSV.dial between air and CTV @17:25:22.26 CS.seek 6.946ml @17:25:30.02 CTV.dial CSV,avoiding => air @17:25:30.04 CSV.dial CTV @17:25:31.38 CS.reconfigure CSpressurize @17:25:31.43 CS.seek 0.000ml @17:25:48.97 delay 2:00 @17:27:22.54 delay 30 seconds @17:27:48.98 CBV.dial :waste2b @17:27:49.31 CS.reconfigure CSconfig @17:27:49.36 CS.seek empty @17:27:50.82 delay 1:00 @17:27:52.55 ATV.dial :waste,via => air3 @17:27:54.09 AS.reconfigure ASconfig @17:27:54.14 AS.seek empty @17:28:11.65 Parking Bleach in LysateSource line and SPEcolumn @17:28:11.66 ATV.dial :air3 @17:28:12.23 AS.reconfigure ASslow1 @17:28:12.28 AS.seek 0.029ml @17:28:18.74 ATV.dial :ElutionWater @17:28:19.13 AS.seek 0.059ml @17:28:25.54 ATV.dial :air3 @17:28:25.93 AS.seek 0.069ml @17:28:28.24 delay 2 seconds @17:28:30.25 ATV.dial :bleach @17:28:30.73 AS.seek 0.711ml @17:28:50.83 CTV.dial :air,avoiding => intake @17:28:52.11 Fix #2 of 2 (with rnal) for 02:00.00 @17:28:52.12 CS Pulling 2ml of rnal @17:28:52.13 CSV.dial CSR @17:28:52.58 CSR.select :rnal @17:28:52.69 CS.seek 2.000ml @17:28:58.69 delay 5 seconds @17:29:03.70 CSR.select :air @17:29:03.81 CS.reconfigure CSfast @17:29:03.86 CS.seek 10.000ml @17:29:22.75 CBV.dial between CSV and waste2b @17:29:23.10 Pumping wcr puck from tube 2 up to 55psi... @17:29:23.16 CBV.dial SSV @17:29:24.60 CBV.dial between CSV and waste2b @17:29:25.57 CSV.dial between air and CTV @17:29:26.24 CS.seek 6.852ml @17:29:34.82 CTV.dial CSV,avoiding => air @17:29:34.84 CSV.dial CTV @17:29:36.28 CS.reconfigure CSpressurize @17:29:36.33 CS.seek 0.000ml @17:29:52.96 delay 2:00 @17:30:41.95 delay 3 seconds @17:30:44.96 ATV.dial :air3 @17:30:45.46 delay 3 seconds @17:30:48.51 AS.seek 0.736ml @17:30:53.86 delay 3 seconds @17:30:56.87 ATV.dial :bleach @17:30:57.25 AS.seek 0.721ml @17:31:00.56 delay 3 seconds @17:31:03.57 ATV.dial :AIV @17:31:04.63 AS.reconfigure ASconfig @17:31:04.67 AS.seek 0.015ml @17:31:46.14 ATV.dial :waste @17:31:47.41 AS.seek empty @17:31:48.62 ATV.dial :air3 @17:31:49.19 AS.reconfigure ASslow1 @17:31:49.23 AS.seek 0.009ml @17:31:51.59 delay 2 seconds @17:31:52.97 CBV.dial :waste2b @17:31:53.30 CS.reconfigure CSconfig @17:31:53.35 CS.seek empty @17:31:53.60 ATV.dial :bleach @17:31:53.98 AS.seek 0.579ml @17:31:55.24 delay 1:00 @17:32:55.25 CTV.dial :air,avoiding => intake @17:32:56.59 Storing wcr puck in Collection Clamp to tube 1 @17:32:56.73 SC.reconfigure SCtight @17:32:56.78 SC.seek 1 @17:32:58.99 SC.reconfigure SCloose @17:32:59.18 Elbow.reconfigure TightElbow @17:32:59.23 Elbow.seek 1 @17:33:00.88 Forearm.reconfigure TightForearm @17:33:00.93 Forearm.seek 1 + 800 counts @17:33:02.02 SE.reconfigure SEpick @17:33:02.09 SE.seek beamBreak @17:33:04.33 SE.configure SEconfig @17:33:04.47 Elbow.reconfigure LimpElbow @17:33:04.52 Forearm.reconfigure LooseForearm @17:33:04.57 Forearm.seek 1 @17:33:04.82 Elbow.hold @17:33:04.90 Elbow.reconfigure TightElbow @17:33:04.99 SE.seek 7.04 Pucks below Top Plate @17:33:05.32 Forearm.coast @17:33:05.37 Forearm.seek 1 + 346 counts @17:33:05.62 Forearm.reconfigure TightForearm @17:33:05.67 Forearm.seek 1 + 800 counts @17:33:05.97 SE.seek 8.04 Pucks below Top Plate @17:33:06.56 Forearm.reconfigure LooseForearm @17:33:06.61 Forearm.seek retract @17:33:07.55 Elbow.seek CC @17:33:09.13 Elbow.reconfigure LooseElbow @17:33:09.23 Forearm.seek CC @17:33:10.15 CC.seek sealed @17:33:11.11 Hand.grip @17:33:11.86 CC.seek open @17:33:15.22 Forearm.seek retract @17:33:16.20 Elbow.reconfigure TightElbow @17:33:16.25 Elbow.seek 1 @17:33:17.89 Elbow.reconfigure LimpElbow @17:33:17.95 Forearm.seek 1 @17:33:18.94 Elbow.hold @17:33:19.02 Elbow.reconfigure TightElbow @17:33:19.07 Hand.release @17:33:19.94 Forearm.coast @17:33:19.99 Forearm.seek 1 + 330 counts @17:33:20.87 Forearm.reconfigure TightForearm @17:33:20.92 Forearm.seek 1 + 800 counts @17:33:21.15 SE.seek down @17:33:22.26 Forearm.reconfigure LooseForearm @17:33:22.31 Forearm.seek retract @17:33:23.13 SE.coast @17:33:23.24 SC.coast @17:33:23.29 Starting finalPurge @17:33:23.30 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:33:23.47 Elbow.seek FlushPuck::Garage @17:33:25.21 Elbow.reconfigure LooseElbow @17:33:25.31 Forearm.seek garage @17:33:26.36 Hand.grip @17:33:27.20 Forearm.seek retract @17:33:28.23 Elbow.reconfigure TightElbow @17:33:28.27 Elbow.seek CC @17:33:31.05 Elbow.reconfigure LooseElbow @17:33:31.14 Forearm.reconfigure TightForearm @17:33:31.19 Forearm.seek CC @17:33:32.31 CC.seek closed @17:33:36.08 Collection Clamp onSpring/offSpring for 4/21 samples, on/off torque 2.00 @17:33:36.15 Hand.release @17:33:36.90 Forearm.reconfigure LooseForearm @17:33:36.95 Forearm.seek retract @17:33:37.94 Flushing the Collection Valve::Manifold @17:33:38.06 CS Pulling 5ml of flush @17:33:38.07 CSV.dial CSR @17:33:38.50 CSR.select :flush @17:33:38.62 CS.seek 5.000ml @17:33:50.49 delay 3 seconds @17:33:52.24
delay 5 seconds @17:33:53.50 ATV.dial :air3 @17:33:53.94 delay 3 seconds @17:33:56.99 AS.seek 0.604ml @17:33:57.26
CSR.select :air @17:33:57.42 CS.reconfigure CSfast @17:33:57.46 CS.seek 7.501ml @17:34:02.35 delay 3 seconds @17:34:03.99
CSV.dial :waste @17:34:05.26 CS.reconfigure CSconfig @17:34:05.30 CS.seek 0.000ml @17:34:05.36 ATV.dial :bleach @17:34:05.75 AS.seek 0.589ml @17:34:09.04 delay 3 seconds @17:34:12.05 ATV.dial :ASV @17:34:12.93 ASV.dial :wasteR @17:34:13.61 AS.seek 0.000ml @17:34:25.21
Flushing lower manifold's waste1 port @17:34:25.32 CSV.dial :flush @17:34:25.78 CS.reconfigure CSfast @17:34:25.82 CS.seek 5.000ml @17:34:37.95 delay 5 seconds @17:34:42.96 CSV.dial :air @17:34:43.66 CS.seek 7.000ml @17:34:49.14 CSV.dial CTV @17:34:49.18
CTV.dial CSV @17:34:50.50 CBV.dial :waste1 @17:34:51.28 CS.seek 0.000ml @17:35:07.70 Flushing lower manifold's waste2b port @17:35:07.82 CSV.dial :flush @17:35:08.62 CS.seek 5.000ml @17:35:20.77 delay 5 seconds @17:35:25.78 CSV.dial :air @17:35:26.35 CS.seek 7.000ml @17:35:31.86 CSV.dial CTV @17:35:31.90
CTV.dial CSV @17:35:32.34 CBV.dial :waste2b @17:35:33.10 CS.seek 0.000ml @17:35:50.42 Flushing upper manifold @17:35:50.54 CSV.dial :flush @17:35:51.35 CS.seek 5.000ml @17:36:03.39 delay 5 seconds @17:36:08.40 CSV.dial :air @17:36:08.97 CS.seek 7.000ml @17:36:14.02 delay 1:00 @17:36:14.36 CSV.dial CBV @17:36:14.40
CBV.dial CSV @17:36:15.51 CTV.dial :air @17:36:16.85 CS.seek 0.000ml @17:36:33.39 delay 7 seconds @17:36:40.40 CS.seek 3.000ml @17:36:48.01 CSV.dial :waste @17:36:48.54 CS.seek 0.000ml @17:36:56.41 CSV.dial :flush @17:36:56.85 CS.seek 3.000ml @17:37:04.45 delay 5 seconds @17:37:09.46 CSV.dial :air @17:37:09.95 CS.seek 5.000ml @17:37:14.03 AFV.select :carrierSolution @17:37:14.13 delay 2 seconds @17:37:15.39
CSV.dial :air @17:37:15.41 CS.seek 0.000ml @17:37:16.14 AS.reconfigure ASconfig @17:37:16.19 AS.seek 1.000ml @17:37:27.45
delay 7 seconds @17:37:34.46 CS.seek 3.000ml @17:37:42.10 CSV.dial :waste @17:37:42.91 CS.seek 0.000ml @17:37:50.54 Disinfecting external Intake with mfbkill @17:37:50.65 CSV.dial CSR @17:37:51.91 CSR.select :mfbkill @17:37:52.02 CS.seek 8.000ml @17:38:10.94 delay 5 seconds @17:38:14.81 delay 1 second @17:38:15.82 AFV.select :holdingCoil @17:38:15.92 delay 1 second @17:38:15.95
CSR.select :air @17:38:16.06 CS.seek 10.000ml @17:38:16.93 ATV.dial :ASV @17:38:16.94 ASV.dial :wasteR @17:38:16.95 AS.reconfigure ASslow1 @17:38:17.01 AS.seek 0.000ml @17:38:21.44 CSV.dial CBV @17:38:21.47
CBV.dial CSV @17:38:22.52 CTV.dial :intake @17:38:23.27 Intake.select :open @17:38:23.38 CS.seek 2.000ml @17:38:42.63 Intake.select :close @17:38:42.74 CTV.dial :air @17:38:43.50 CS.reconfigure CSconfig @17:38:43.55 CS.seek 0.000ml @17:38:49.60 Flushing lower manifold's waste1 port @17:38:49.61 CS Pulling 4ml of mfbkill @17:38:49.63 CSV.dial CSR @17:38:51.11 CSR.select :mfbkill @17:38:51.23 CS.seek 4.000ml @17:39:02.16 delay 5 seconds @17:39:07.17 CSR.select :air @17:39:07.27 CS.reconfigure CSfast @17:39:07.32 CS.seek 10.000ml @17:39:21.64 CSV.dial CTV @17:39:21.66
CTV.dial CSV @17:39:23.00 CBV.dial :waste1 @17:39:23.47 CS.seek 0.000ml @17:39:46.53 Flushing lower manifold's waste2b port @17:39:46.54 CS Pulling 4ml of mfbkill @17:39:46.56 CSV.dial CSR @17:39:47.08 CSR.select :mfbkill @17:39:47.19 CS.reconfigure CSconfig @17:39:47.24 CS.seek 4.000ml @17:39:58.16 delay 5 seconds @17:40:03.17 CSR.select :air @17:40:03.27 CS.reconfigure CSfast @17:40:03.32 CS.seek 10.000ml @17:40:17.63 CSV.dial CTV @17:40:17.66
CTV.dial CSV @17:40:18.18 CBV.dial :waste2b @17:40:18.95 CS.seek 0.000ml @17:40:42.03 Killing upper manifold @17:40:42.04 CS Pulling 4ml of mfbkill @17:40:42.05 CSV.dial CSR @17:40:42.58 CSR.select :mfbkill @17:40:42.69 CS.reconfigure CSconfig @17:40:42.74 CS.seek 4.000ml @17:40:53.69 delay 5 seconds @17:40:58.70 CSR.select :air @17:40:58.81 CS.reconfigure CSfast @17:40:58.86 CS.seek 10.000ml @17:41:13.19 CSV.dial CBV @17:41:13.21
CBV.dial CSV @17:41:14.25 CTV.dial :air @17:41:15.55 CS.seek 0.000ml @17:41:38.78 delay 7 seconds @17:41:41.23 delay 20 seconds @17:41:45.79
CS.seek 3.000ml @17:41:53.47 CSV.dial :waste @17:41:53.91 CS.seek 0.000ml @17:42:01.24 ATV.dial :air3 @17:42:01.50
Flushing upper manifold @17:42:01.62 CSV.dial :flush @17:42:02.27 CS.seek 5.000ml @17:42:02.39 AS.seek 0.590ml @17:42:14.37
delay 5 seconds @17:42:19.38 CSV.dial :air @17:42:19.97 CS.seek 7.000ml @17:42:25.38 CSV.dial CBV @17:42:25.42
CBV.dial CSV @17:42:26.50 CTV.dial :air @17:42:26.51 CS.seek 0.000ml @17:42:43.25 delay 7 seconds @17:42:50.26 CS.seek 3.000ml @17:42:57.97 CSV.dial :waste @17:42:58.50 CS.seek 0.000ml @17:43:06.31 CSV.dial :flush @17:43:06.75 CS.seek 3.000ml @17:43:14.32 delay 5 seconds @17:43:19.33 CSV.dial :air @17:43:19.82 CS.seek 4.999ml @17:43:25.19 CSV.dial :air @17:43:25.21 CS.seek 0.000ml @17:43:37.31 delay 7 seconds @17:43:44.32 CS.seek 3.000ml @17:43:51.97 CSV.dial :waste @17:43:52.78 CS.seek 0.000ml @17:44:00.41 Flushing lower manifold's waste1 port @17:44:00.52 CSV.dial :flush @17:44:01.04 CS.seek 5.000ml @17:44:03.01 delay 3 seconds @17:44:06.02 ATV.dial :ASV @17:44:07.09 ASV.dial :wasteR @17:44:07.16 AS.seek 0.090ml @17:44:13.16
delay 5 seconds @17:44:18.17 CSV.dial :air @17:44:18.66 CS.seek 7.000ml @17:44:24.09 CSV.dial CTV @17:44:24.13
CTV.dial CSV @17:44:25.47 CBV.dial :waste1 @17:44:25.93 CS.seek 0.000ml @17:44:42.41 Flushing lower manifold's waste2b port @17:44:42.53 CSV.dial :flush @17:44:43.34 CS.seek 5.000ml @17:44:55.34 delay 5 seconds @17:45:00.35 CSV.dial :air @17:45:01.02 CS.seek 7.000ml @17:45:06.37 CSV.dial CTV @17:45:06.41
CTV.dial CSV @17:45:06.85 CBV.dial :waste2b @17:45:07.62 CS.seek 0.000ml @17:45:24.06 Disinfecting external Intake with kill @17:45:24.17 CSV.dial CSR @17:45:24.61 CSR.select :kill @17:45:24.72 CS.seek 8.000ml @17:45:43.53 delay 5 seconds @17:45:48.54 CSR.select :air @17:45:48.65 CS.seek 10.000ml @17:45:49.42 delay 30 seconds @17:45:54.03 CSV.dial CBV @17:45:54.05
CBV.dial CSV @17:45:55.10 CTV.dial :intake @17:45:55.82 Intake.select :open @17:45:55.93 CS.seek 2.000ml @17:46:15.18 Intake.select :close @17:46:15.29 CTV.dial :air @17:46:16.02 CS.reconfigure CSconfig @17:46:16.06 CS.seek 0.000ml @17:46:19.43 ATV.dial :extSrc @17:46:19.95 delay 3 seconds @17:46:21.96
CSV.dial CSR @17:46:22.96 ATV.dial :ElutionWater @17:46:23.04
CSR.select :flush @17:46:23.15 CS.seek 5.000ml @17:46:23.87 AS.seek 0.120ml @17:46:30.24 ATV.dial :air3 @17:46:30.64 AS.seek 0.130ml @17:46:32.85 delay 2 seconds @17:46:34.86 ATV.dial :bleach @17:46:35.23 AS.seek 0.230ml @17:46:36.59
delay 5 seconds @17:46:41.60 CSR.select :air @17:46:41.71 CS.seek 10.000ml @17:46:55.16 CSV.dial CBV @17:46:55.90 delay 3 seconds @17:46:56.46
CS.seek 0.000ml @17:46:58.91 ATV.dial :air3 @17:46:59.25 delay 3 seconds @17:47:02.30 AS.seek 0.255ml @17:47:07.65 delay 3 seconds @17:47:10.66 ATV.dial :bleach @17:47:11.55 AS.seek 0.240ml @17:47:14.88 delay 3 seconds @17:47:17.89 ATV.dial :ASV @17:47:18.87 ASV.dial :SPEdelivery @17:47:19.35 AFV.select :carrierSolution @17:47:19.45 delay 2 seconds @17:47:21.46 AS.reconfigure ASconfig @17:47:21.51 AS.seek 0.500ml @17:47:22.55
Garaging FlushPuck in Collection Clamp @17:47:22.63 Elbow.reconfigure TightElbow @17:47:22.67 Elbow.seek CC @17:47:22.77 Elbow.reconfigure LooseElbow @17:47:22.87 Forearm.seek CC @17:47:23.83 CC.seek sealed @17:47:24.77 Hand.grip @17:47:25.58 CC.seek open @17:47:28.90 Forearm.seek retract @17:47:29.88 Elbow.reconfigure TightElbow @17:47:29.92 Elbow.seek garage @17:47:32.79 Elbow.reconfigure LooseElbow @17:47:32.88 Forearm.reconfigure TightForearm @17:47:32.93 Forearm.seek garage @17:47:34.12 Hand.release @17:47:34.87 Forearm.reconfigure LooseForearm @17:47:34.92 Forearm.seek retract @17:47:35.96 Completed {coreNegNTCphase} begun 16:20:41 @17:47:35.97 deferred until MFBPCR finish @17:47:35.99
Waiting for MFBPCR after completing 14jan21SCCWRP assays @17:47:36.01 GoodNews.email "",Subject => "Waiting for MFBPCR after completing 14jan21SCCWRP assays" @17:47:37.55 delay 1 second @17:47:38.56 AFV.select :holdingCoil @17:47:38.72 AS.reconfigure ASslow1 @17:47:38.77 AS.seek 0.170ml @17:48:46.35 SPE.hold 2:46:40,95.0C @17:48:46.37 delay 3:00 @17:51:46.39 AFV.select :carrierSolution @17:51:46.49 delay 2 seconds @17:51:48.50 AS.reconfigure ASconfig @17:51:48.55 AS.seek 1.000ml @17:52:37.23 delay 1 second @17:52:38.24 AFV.select :holdingCoil @17:52:38.35 delay 1 second @17:52:39.36 ASV.dial :wasteD @17:52:40.47 AS.seek 0.000ml @17:53:39.07 Priming Eluate @17:53:39.08 AFV.select :carrierSolution @17:53:39.19 delay 2 seconds @17:53:41.20 AS.seek 0.500ml @17:54:10.60 delay 1 second @17:54:11.61 AFV.select :holdingCoil @17:54:11.71 ATV.dial ASV @17:54:11.72 ASV.dial :SPErecovery @17:54:12.38 Swish #1 of 2 @17:54:12.45 AS.reconfigure ASslow0 @17:54:12.50 AS.seek 0.600ml @17:54:23.00 AS.seek 0.500ml @17:54:33.51 Swish #2 of 2 @17:54:33.58 AS.seek 0.600ml @17:54:44.14 AS.seek 0.500ml @17:54:54.63 delay 3 seconds @17:54:57.69 AS.reconfigure ASslow1 @17:54:57.73 AS.seek 0.676ml @17:55:33.88 delay 3 seconds @17:55:36.89 Eluate primed @17:55:36.90 ATV.dial :waste2 @17:55:37.44 AS.reconfigure ASnorm @17:55:37.48 AS.seek 0.000ml @17:56:12.35 SPE.coast @17:56:12.43 ASV.dial :SPErecovery @17:56:12.45 Setting up PCR for HF183em -- writing /var/log/esp/HF183em14jan2317h.pcr @17:56:12.46 Building PCR Slug from ASV with MM2 mix, PP4 primer @17:56:12.48 Priming with carrier @17:56:12.49 ATV.dial ARV @17:56:13.21 ARV.dial :carrier @17:56:13.51 AS.seek 0.500ml @17:56:39.42 AFV.select :carrierSolution @17:56:39.53 delay 2 seconds @17:56:41.54 AS.reconfigure ASconfig @17:56:41.59 AS.seek 1.000ml @17:57:11.05 delay 1 second @17:57:12.06 AFV.select :holdingCoil @17:57:12.16 delay 1 second @17:57:13.17 ATV.dial :waste @17:57:14.43 AS.reconfigure ASnorm @17:57:14.48 AS.seek empty @17:58:05.98 AFV.select :carrierSolution @17:58:06.09 delay 2 seconds @17:58:08.10 AS.reconfigure ASconfig @17:58:08.15 AS.seek 0.500ml @17:58:38.11 delay 1 second @17:58:39.12 AFV.select :holdingCoil @17:58:39.22 Building Slug @17:58:39.23 ATV.dial :ARV @17:58:40.48 delay 2 seconds @17:58:42.49 ARV.dial :air1 @17:58:42.83 delay 2 seconds @17:58:44.89 AS.reconfigure ASslow2 @17:58:44.94 AS.seek 0.506ml @17:58:51.34 delay 2 seconds @17:58:53.35 ARV.dial :MM2 @17:58:54.69 delay 2 seconds @17:58:56.70 Adding MM2 @17:58:56.77 AS.seek 0.524ml @17:59:15.34 delay 25 seconds @17:59:40.36 ARV.dial :PP4 @17:59:40.83 Adding PP4 @17:59:40.84 delay 2 seconds @17:59:42.90 AS.seek 0.530ml @17:59:49.27 delay 10 seconds @17:59:59.28 ARV.dial :air3 @17:59:59.62 adding ARV air @17:59:59.63 delay 2 seconds @18:00:01.69 AS.seek 0.536ml @18:00:08.02 delay 5 seconds @18:00:13.03 ARV.dial :carrier @18:00:14.08 delay 7 seconds @18:00:21.14 AS.seek 0.594ml @18:01:20.61 delay 5 seconds @18:01:25.62 ATV.dial :ASV @18:01:26.03 delay 2 seconds @18:01:28.09 AS.seek 0.600ml @18:01:34.36 delay 4 seconds @18:01:38.37 ATV.dial ARV @18:01:38.71 delay 2 seconds @18:01:40.72 ARV.dial :carrier @18:01:40.73 delay 2 seconds @18:01:42.79 AS.seek 0.650ml @18:02:34.02 delay 4 seconds @18:02:38.03 ATV.dial PCR @18:02:38.53 ASV.dial :waste1 @18:02:39.22 ARV.dial :air1 @18:02:39.56 Positioning Slug by 0.324ml @18:02:39.63 AS.seek 0.326ml @18:08:10.15 delay 5 seconds @18:08:15.16 ATV.dial ARV @18:08:15.79 ASV.dial :PCRlock @18:08:16.15 delay 5 seconds @18:08:21.16 Running HF183em ProfileEM60C2 thermocycling @18:08:21.17 PCR.program ProfileEM60C2 @18:08:21.30 ProfileEM60C2 should complete in < 2:11:38 @18:08:21.31 PCR.run 2:49:32.5 @20:05:39.29 PCR.off @20:05:39.82 PCR for HF183em completed -- closed /var/log/esp/HF183em14jan2317h.pcr @20:05:39.88 Cleaning PCR @20:05:39.89 AFV.select :carrierSolution @20:05:39.99 delay 2 seconds @20:05:42.00 AS.reconfigure ASconfig @20:05:42.05 AS.seek 0.500ml @20:05:52.51 delay 1 second @20:05:53.52 AFV.select :holdingCoil @20:05:53.62 ASV.dial :waste1 @20:05:54.38 ATV.dial :air3 @20:05:55.32 AS.reconfigure ASslow1 @20:05:55.37 AS.seek 0.510ml @20:05:57.65 delay 2 seconds @20:05:59.66 ATV.dial :bleach @20:06:00.15 AS.seek 0.810ml @20:07:01.60 delay 3 seconds @20:07:04.61 ATV.dial :air3 @20:07:04.94 delay 3 seconds @20:07:08.00 AS.seek 0.835ml @20:07:13.35 delay 3 seconds @20:07:16.36 ATV.dial :bleach @20:07:16.86 AS.seek 0.820ml @20:07:20.19 delay 3 seconds @20:07:23.20 ARV.dial :waste @20:07:23.71 delay 3 seconds @20:07:26.72 ATV.dial :ARV @20:07:27.56 AS.reconfigure ASconfig @20:07:27.61 AS.seek 0.470ml @20:07:48.31 AFV.select :carrierSolution @20:07:48.42 delay 2 seconds @20:07:50.43 AS.seek 1.000ml @20:08:21.73 delay 1 second @20:08:22.74 AFV.select :holdingCoil @20:08:22.84 delay 1 second @20:08:23.85 AS.reconfigure ASfast @20:08:23.90 AS.seek 0.000ml @20:08:51.62 AFV.select :carrierSolution @20:08:51.73 delay 2 seconds @20:08:53.74 AS.reconfigure ASconfig @20:08:53.79 AS.seek 1.000ml @20:09:52.40 delay 1 second @20:09:53.41 AFV.select :holdingCoil @20:09:53.51 delay 1 second @20:09:54.52 AS.reconfigure ASfast @20:09:54.57 AS.seek 0.000ml @20:10:22.32 AFV.select :carrierSolution @20:10:22.43 delay 2 seconds @20:10:24.44 AS.reconfigure ASconfig @20:10:24.49 AS.seek 1.000ml @20:11:23.12 delay 1 second @20:11:24.13 AFV.select :holdingCoil @20:11:24.23 delay 1 second @20:11:25.28 AS.reconfigure ASfast @20:11:25.40 AS.seek 0.000ml @20:11:53.13 ARV.dial between carrier and air1 @20:11:53.56 ATV.dial :air3 @20:11:54.59 AS.reconfigure ASslow1 @20:11:54.64 AS.seek 0.010ml @20:11:57.01 delay 2 seconds @20:11:59.02 ATV.dial :bleach @20:11:59.40 AS.seek 0.810ml @20:14:42.72 delay 3 seconds @20:14:45.73 ATV.dial :air3 @20:14:46.07 delay 3 seconds @20:14:49.13 AS.seek 0.835ml @20:14:54.50 delay 3 seconds @20:14:57.51 ATV.dial :bleach @20:14:57.90 AS.seek 0.820ml @20:15:01.21 delay 3 seconds @20:15:04.22 ATV.dial PCR @20:15:04.56 AS.reconfigure ASconfig @20:15:04.60 AS.seek empty @20:15:52.71 PCR.program :bleachProfile @20:15:52.77 PCR program should complete in < 2:45 @20:15:52.78 PCR.run 10:00 @20:18:50.98 PCR.off @20:18:51.01 AFV.select :carrierSolution @20:18:51.11 delay 2 seconds @20:18:53.12 AS.seek 1.000ml @20:19:51.78 delay 1 second @20:19:52.79 AFV.select :holdingCoil @20:19:52.89 delay 1 second @20:19:53.90 AS.reconfigure ASfast @20:19:53.95 AS.seek 0.000ml @20:20:21.70 delay 3 seconds @20:20:24.71 AFV.select :carrierSolution @20:20:24.81 delay 2 seconds @20:20:26.82 AS.reconfigure ASconfig @20:20:26.87 AS.seek 1.000ml @20:21:25.50 delay 1 second @20:21:26.51 AFV.select :holdingCoil @20:21:26.61 delay 1 second @20:21:27.62 AS.reconfigure ASfast @20:21:27.67 AS.seek 0.000ml @20:21:55.42 delay 3 seconds @20:21:58.43 AFV.select :carrierSolution @20:21:58.54 delay 2 seconds @20:22:00.55 AS.reconfigure ASconfig @20:22:00.60 AS.seek 1.000ml @20:22:59.23 delay 1 second @20:23:00.24 AFV.select :holdingCoil @20:23:00.35 delay 1 second @20:23:01.36 AS.reconfigure ASfast @20:23:01.41 AS.seek 0.000ml @20:23:29.14 delay 3 seconds @20:23:32.15 AFV.select :carrierSolution @20:23:32.26 delay 2 seconds @20:23:34.27 AS.reconfigure ASconfig @20:23:34.32 AS.seek 1.000ml @20:24:32.91 delay 1 second @20:24:33.92 AFV.select :holdingCoil @20:24:34.02 delay 1 second @20:24:35.03 AS.reconfigure ASfast @20:24:35.07 AS.seek 0.000ml @20:25:02.80 delay 3 seconds @20:25:05.81 AFV.select :carrierSolution @20:25:06.36 delay 2 seconds @20:25:08.36 AS.reconfigure ASconfig @20:25:08.41 AS.seek 1.000ml @20:26:07.04 delay 1 second @20:26:08.05 AFV.select :holdingCoil @20:26:08.15 delay 1 second @20:26:09.18 AS.reconfigure ASnorm @20:26:09.27 AS.seek 0.000ml @20:27:00.80 Setting up PCR for ENTap -- writing /var/log/esp/ENTap14jan2320h.pcr @20:27:00.81 Building PCR Slug from ASV with MM1 mix, PP1 primer @20:27:00.82 Priming with carrier @20:27:00.84 ATV.dial ARV @20:27:01.36 ARV.dial :carrier @20:27:01.66 AS.seek 0.500ml @20:27:27.55 AFV.select :carrierSolution @20:27:27.66 delay 2 seconds @20:27:29.67 AS.reconfigure ASconfig @20:27:29.72 AS.seek 1.000ml @20:27:59.17 delay 1 second @20:28:00.18 AFV.select :holdingCoil @20:28:00.28 delay 1 second @20:28:01.29 ATV.dial :waste @20:28:02.54 AS.reconfigure ASnorm @20:28:02.59 AS.seek empty @20:28:54.08 AFV.select :carrierSolution @20:28:54.19 delay 2 seconds @20:28:56.20 AS.reconfigure ASconfig @20:28:56.25 AS.seek 0.500ml @20:29:25.75 delay 1 second @20:29:26.76 AFV.select :holdingCoil @20:29:26.86 Building Slug @20:29:26.87 ATV.dial :ARV @20:29:28.12 delay 2 seconds @20:29:30.13 ARV.dial :air1 @20:29:30.47 delay 2 seconds @20:29:32.53 AS.reconfigure ASslow2 @20:29:32.58 AS.seek 0.506ml @20:29:39.09 delay 2 seconds @20:29:41.10 ARV.dial :MM1 @20:29:41.61 delay 2 seconds @20:29:43.62 Adding MM1 @20:29:43.68 AS.seek 0.524ml @20:30:02.36 delay 25 seconds @20:30:27.37 ARV.dial :PP1 @20:30:27.72 Adding PP1 @20:30:27.73 delay 2 seconds @20:30:29.80 AS.seek 0.530ml @20:30:36.28 delay 10 seconds @20:30:46.29 ARV.dial :air1 @20:30:46.63 adding ARV air @20:30:46.64 delay 2 seconds @20:30:48.70 AS.seek 0.536ml @20:30:55.00 delay 5 seconds @20:31:00.01 ARV.dial :carrier @20:31:00.34 delay 7 seconds @20:31:07.40 AS.seek 0.594ml @20:32:06.91 delay 5 seconds @20:32:11.92 ATV.dial :ASV @20:32:12.24 delay 2 seconds @20:32:14.30 AS.seek 0.600ml @20:32:20.65 delay 4 seconds @20:32:24.66 ATV.dial ARV @20:32:25.01 delay 2 seconds @20:32:27.02 ARV.dial :carrier @20:32:27.03 delay 2 seconds @20:32:29.09 AS.seek 0.650ml @20:33:20.19 delay 4 seconds @20:33:24.20 ATV.dial PCR @20:33:24.70 ASV.dial :waste1 @20:33:24.71 ARV.dial :air1 @20:33:25.07 Positioning Slug by 0.324ml @20:33:25.14 AS.seek 0.326ml @20:38:55.79 delay 5 seconds @20:39:00.80 ATV.dial ARV @20:39:01.41 ASV.dial :PCRlock @20:39:01.94 delay 5 seconds @20:39:06.95 Running ENTap Profile60C thermocycling @20:39:06.96 PCR.program Profile60C @20:39:07.08 Profile60C should complete in < 1:39:08 @20:39:07.09 PCR.run 2:08:55 @22:03:17.53 PCR.off @22:03:18.06 PCR for ENTap completed -- closed /var/log/esp/ENTap14jan2320h.pcr @22:03:18.12 Cleaning PCR @22:03:18.13 AFV.select :carrierSolution @22:03:18.23 delay 2 seconds @22:03:20.24 AS.reconfigure ASconfig @22:03:20.29 AS.seek 0.500ml @22:03:30.77 delay 1 second @22:03:31.78 AFV.select :holdingCoil @22:03:31.89 ASV.dial :waste1 @22:03:32.29 ATV.dial :air3 @22:03:33.32 AS.reconfigure ASslow1 @22:03:33.37 AS.seek 0.510ml @22:03:35.69 delay 2 seconds @22:03:37.70 ATV.dial :bleach @22:03:38.09 AS.seek 0.810ml @22:04:39.55 delay 3 seconds @22:04:42.56 ATV.dial :air3 @22:04:42.89 delay 3 seconds @22:04:45.95 AS.seek 0.835ml @22:04:51.30 delay 3 seconds @22:04:54.31 ATV.dial :bleach @22:04:54.69 AS.seek 0.820ml @22:04:58.00 delay 3 seconds @22:05:01.01 ARV.dial :waste @22:05:01.52 delay 3 seconds @22:05:04.53 ATV.dial :ARV @22:05:05.27 AS.reconfigure ASconfig @22:05:05.32 AS.seek 0.470ml @22:05:26.00 AFV.select :carrierSolution @22:05:26.11 delay 2 seconds @22:05:28.12 AS.seek 1.000ml @22:05:59.33 delay 1 second @22:06:00.34 AFV.select :holdingCoil @22:06:00.44 delay 1 second @22:06:01.45 AS.reconfigure ASfast @22:06:01.50 AS.seek 0.000ml @22:06:29.22 AFV.select :carrierSolution @22:06:29.33 delay 2 seconds @22:06:31.34 AS.reconfigure ASconfig @22:06:31.39 AS.seek 1.000ml @22:07:29.98 delay 1 second @22:07:30.99 AFV.select :holdingCoil @22:07:31.10 delay 1 second @22:07:32.11 AS.reconfigure ASfast @22:07:32.16 AS.seek 0.000ml @22:07:59.87 AFV.select :carrierSolution @22:07:59.98 delay 2 seconds @22:08:01.99 AS.reconfigure ASconfig @22:08:02.04 AS.seek 1.000ml @22:09:00.67 delay 1 second @22:09:01.68 AFV.select :holdingCoil @22:09:01.79 delay 1 second @22:09:02.80 AS.reconfigure ASfast @22:09:02.85 AS.seek 0.000ml @22:09:30.57 ARV.dial between carrier and air1 @22:09:30.99 ATV.dial :air3 @22:09:31.92 AS.reconfigure ASslow1 @22:09:31.97 AS.seek 0.010ml @22:09:34.32 delay 2 seconds @22:09:36.33 ATV.dial :bleach @22:09:36.72 AS.seek 0.810ml @22:12:20.11 delay 3 seconds @22:12:23.12 ATV.dial :air3 @22:12:23.46 delay 3 seconds @22:12:26.52 AS.seek 0.835ml @22:12:31.89 delay 3 seconds @22:12:34.90 ATV.dial :bleach @22:12:35.29 AS.seek 0.820ml @22:12:38.60 delay 3 seconds @22:12:41.61 ATV.dial PCR @22:12:42.04 AS.reconfigure ASconfig @22:12:42.09 AS.seek empty @22:13:30.25 PCR.program :bleachProfile @22:13:30.31 PCR program should complete in < 2:45 @22:13:30.32 PCR.run 10:00 @22:16:28.53 PCR.off @22:16:28.57 AFV.select :carrierSolution @22:16:28.67 delay 2 seconds @22:16:30.68 AS.seek 1.000ml @22:17:29.33 delay 1 second @22:17:30.34 AFV.select :holdingCoil @22:17:30.44 delay 1 second @22:17:31.45 AS.reconfigure ASfast @22:17:31.50 AS.seek 0.000ml @22:17:59.24 delay 3 seconds @22:18:02.79 AFV.select :carrierSolution @22:18:02.89 delay 2 seconds @22:18:04.90 AS.reconfigure ASconfig @22:18:04.94 AS.seek 1.000ml @22:19:03.52 delay 1 second @22:19:04.53 AFV.select :holdingCoil @22:19:04.64 delay 1 second @22:19:05.65 AS.reconfigure ASfast @22:19:05.70 AS.seek 0.000ml @22:19:33.45 delay 3 seconds @22:19:36.46 AFV.select :carrierSolution @22:19:36.56 delay 2 seconds @22:19:38.57 AS.reconfigure ASconfig @22:19:38.62 AS.seek 1.000ml @22:20:37.27 delay 1 second @22:20:38.27 AFV.select :holdingCoil @22:20:38.37 delay 1 second @22:20:39.38 AS.reconfigure ASfast @22:20:39.43 AS.seek 0.000ml @22:21:07.18 delay 3 seconds @22:21:10.19 AFV.select :carrierSolution @22:21:10.29 delay 2 seconds @22:21:12.30 AS.reconfigure ASconfig @22:21:12.35 AS.seek 1.000ml @22:22:10.99 delay 1 second @22:22:12.00 AFV.select :holdingCoil @22:22:12.10 delay 1 second @22:22:13.11 AS.reconfigure ASfast @22:22:13.16 AS.seek 0.000ml @22:22:40.90 delay 3 seconds @22:22:43.91 AFV.select :carrierSolution @22:22:44.01 delay 2 seconds @22:22:46.02 AS.reconfigure ASconfig @22:22:46.07 AS.seek 1.000ml @22:23:44.72 delay 1 second @22:23:45.73 AFV.select :holdingCoil @22:23:45.83 delay 1 second @22:23:46.84 AS.reconfigure ASnorm @22:23:46.89 AS.seek 0.000ml @22:24:38.36 Setting up PCR for ENTem -- writing /var/log/esp/ENTem14jan2322h.pcr @22:24:38.37 Building PCR Slug from ASV with MM2 mix, PP3 primer @22:24:38.38 Priming with carrier @22:24:38.40 ATV.dial ARV @22:24:38.93 ARV.dial :carrier @22:24:39.24 AS.seek 0.500ml @22:25:05.17 AFV.select :carrierSolution @22:25:05.28 delay 2 seconds @22:25:07.29 AS.reconfigure ASconfig @22:25:07.34 AS.seek 1.000ml @22:25:36.80 delay 1 second @22:25:37.81 AFV.select :holdingCoil @22:25:37.91 delay 1 second @22:25:38.92 ATV.dial :waste @22:25:40.18 AS.reconfigure ASnorm @22:25:40.23 AS.seek empty @22:26:31.70 AFV.select :carrierSolution @22:26:31.81 delay 2 seconds @22:26:33.82 AS.reconfigure ASconfig @22:26:33.87 AS.seek 0.500ml @22:27:03.37 delay 1 second @22:27:04.38 AFV.select :holdingCoil @22:27:04.48 Building Slug @22:27:04.49 ATV.dial :ARV @22:27:05.73 delay 2 seconds @22:27:07.74 ARV.dial :air1 @22:27:08.08 delay 2 seconds @22:27:10.14 AS.reconfigure ASslow2 @22:27:10.19 AS.seek 0.506ml @22:27:16.50 delay 2 seconds @22:27:18.51 ARV.dial :MM2 @22:27:19.75 delay 2 seconds @22:27:21.76 Adding MM2 @22:27:21.82 AS.seek 0.524ml @22:27:40.50 delay 25 seconds @22:28:05.51 ARV.dial :PP3 @22:28:05.87 Adding PP3 @22:28:05.88 delay 2 seconds @22:28:07.94 AS.seek 0.530ml @22:28:14.29 delay 10 seconds @22:28:24.30 ARV.dial :air2 @22:28:24.74 adding ARV air @22:28:24.75 delay 2 seconds @22:28:26.81 AS.seek 0.536ml @22:28:33.07 delay 5 seconds @22:28:38.08 ARV.dial :carrier @22:28:39.12 delay 7 seconds @22:28:46.18 AS.seek 0.594ml @22:29:45.51 delay 5 seconds @22:29:50.52 ATV.dial :ASV @22:29:51.04 delay 2 seconds @22:29:53.10 AS.seek 0.600ml @22:29:59.56 delay 4 seconds @22:30:03.57 ATV.dial ARV @22:30:03.88 delay 2 seconds @22:30:05.89 ARV.dial :carrier @22:30:05.90 delay 2 seconds @22:30:07.97 AS.seek 0.650ml @22:30:59.30 delay 4 seconds @22:31:03.31 ATV.dial PCR @22:31:03.82 ASV.dial :waste1 @22:31:03.83 ARV.dial :air1 @22:31:04.19 Positioning Slug by 0.324ml @22:31:04.26 AS.seek 0.326ml @22:36:34.91 delay 5 seconds @22:36:39.92 ATV.dial ARV @22:36:40.55 ASV.dial :PCRlock @22:36:41.05 delay 5 seconds @22:36:46.06 Running ENTem ProfileEM60C thermocycling @22:36:46.07 PCR.program ProfileEM60C @22:36:46.20 ProfileEM60C should complete in < 2:11:38 @22:36:46.21 PCR.run 2:49:32.5 @00:34:01.22PST24-Jan-14 PCR.off @00:34:01.75 PCR for ENTem completed -- closed /var/log/esp/ENTem14jan2322h.pcr @00:34:01.82 Cleaning PCR @00:34:01.83 AFV.select :carrierSolution @00:34:01.94 delay 2 seconds @00:34:03.95 AS.reconfigure ASconfig @00:34:04.00 AS.seek 0.500ml @00:34:14.46 delay 1 second @00:34:15.47 AFV.select :holdingCoil @00:34:15.58 ASV.dial :waste1 @00:34:16.33 ATV.dial :air3 @00:34:17.27 AS.reconfigure ASslow1 @00:34:17.32 AS.seek 0.510ml @00:34:19.54 delay 2 seconds @00:34:21.55 ATV.dial :bleach @00:34:22.04 AS.seek 0.810ml @00:35:23.49 delay 3 seconds @00:35:26.50 ATV.dial :air3 @00:35:26.91 delay 3 seconds @00:35:29.97 AS.seek 0.835ml @00:35:35.35 delay 3 seconds @00:35:38.36 ATV.dial :bleach @00:35:38.74 AS.seek 0.820ml @00:35:42.05 delay 3 seconds @00:35:45.06 ARV.dial :waste @00:35:45.66 delay 3 seconds @00:35:48.67 ATV.dial :ARV @00:35:49.41 AS.reconfigure ASconfig @00:35:49.46 AS.seek 0.470ml @00:36:10.14 AFV.select :carrierSolution @00:36:10.25 delay 2 seconds @00:36:12.26 AS.seek 1.000ml @00:36:43.50 delay 1 second @00:36:44.51 AFV.select :holdingCoil @00:36:44.61 delay 1 second @00:36:45.62 AS.reconfigure ASfast @00:36:45.67 AS.seek 0.000ml @00:37:13.41 AFV.select :carrierSolution @00:37:13.52 delay 2 seconds @00:37:15.53 AS.reconfigure ASconfig @00:37:15.58 AS.seek 1.000ml @00:38:14.17 delay 1 second @00:38:15.18 AFV.select :holdingCoil @00:38:15.28 delay 1 second @00:38:16.29 AS.reconfigure ASfast @00:38:16.34 AS.seek 0.000ml @00:38:44.07 AFV.select :carrierSolution @00:38:44.18 delay 2 seconds @00:38:46.19 AS.reconfigure ASconfig @00:38:46.24 AS.seek 1.000ml @00:39:44.85 delay 1 second @00:39:45.86 AFV.select :holdingCoil @00:39:45.96 delay 1 second @00:39:46.97 AS.reconfigure ASfast @00:39:47.02 AS.seek 0.000ml @00:40:14.73 ARV.dial between carrier and air1 @00:40:15.15 ATV.dial :air3 @00:40:16.09 AS.reconfigure ASslow1 @00:40:16.14 AS.seek 0.010ml @00:40:18.49 delay 2 seconds @00:40:20.50 ATV.dial :bleach @00:40:20.89 AS.seek 0.810ml @00:43:04.30 delay 3 seconds @00:43:07.31 ATV.dial :air3 @00:43:07.64 delay 3 seconds @00:43:10.70 AS.seek 0.835ml @00:43:15.99 delay 3 seconds @00:43:19.00 ATV.dial :bleach @00:43:19.38 AS.seek 0.820ml @00:43:23.27 delay 3 seconds @00:43:26.28 ATV.dial PCR @00:43:26.64 AS.reconfigure ASconfig @00:43:26.69 AS.seek empty @00:44:14.82 PCR.program :bleachProfile @00:44:14.87 PCR program should complete in < 2:45 @00:44:14.88 PCR.run 10:00 @00:47:13.08 PCR.off @00:47:13.12 AFV.select :carrierSolution @00:47:13.22 delay 2 seconds @00:47:15.23 AS.seek 1.000ml @00:48:13.91 delay 1 second @00:48:14.92 AFV.select :holdingCoil @00:48:15.03 delay 1 second @00:48:16.04 AS.reconfigure ASfast @00:48:16.09 AS.seek 0.000ml @00:48:43.84 delay 3 seconds @00:48:46.85 AFV.select :carrierSolution @00:48:46.95 delay 2 seconds @00:48:48.96 AS.reconfigure ASconfig @00:48:49.01 AS.seek 1.000ml @00:49:47.64 delay 1 second @00:49:48.65 AFV.select :holdingCoil @00:49:48.76 delay 1 second @00:49:49.77 AS.reconfigure ASfast @00:49:49.82 AS.seek 0.000ml @00:50:17.56 delay 3 seconds @00:50:20.57 AFV.select :carrierSolution @00:50:20.67 delay 2 seconds @00:50:22.68 AS.reconfigure ASconfig @00:50:22.73 AS.seek 1.000ml @00:51:21.35 delay 1 second @00:51:22.36 AFV.select :holdingCoil @00:51:22.47 delay 1 second @00:51:23.48 AS.reconfigure ASfast @00:51:23.52 AS.seek 0.000ml @00:51:51.26 delay 3 seconds @00:51:54.27 AFV.select :carrierSolution @00:51:54.37 delay 2 seconds @00:51:56.38 AS.reconfigure ASconfig @00:51:56.43 AS.seek 1.000ml @00:52:55.04 delay 1 second @00:52:56.05 AFV.select :holdingCoil @00:52:56.16 delay 1 second @00:52:57.17 AS.reconfigure ASfast @00:52:57.22 AS.seek 0.000ml @00:53:24.94 delay 3 seconds @00:53:27.95 AFV.select :carrierSolution @00:53:28.06 delay 2 seconds @00:53:30.07 AS.reconfigure ASconfig @00:53:30.12 AS.seek 1.000ml @00:54:28.72 delay 1 second @00:54:29.73 AFV.select :holdingCoil @00:54:29.83 delay 1 second @00:54:30.84 AS.reconfigure ASnorm @00:54:30.89 AS.seek 0.000ml @00:55:22.33 Setting up PCR for IPCap -- writing /var/log/esp/IPCap14jan2400h.pcr @00:55:22.34 Building PCR Slug from ASV with MM1 mix, PP2 primer @00:55:22.35 Priming with carrier @00:55:22.37 ATV.dial ARV @00:55:22.88 ARV.dial :carrier @00:55:23.18 AS.seek 0.500ml @00:55:49.08 AFV.select :carrierSolution @00:55:49.19 delay 2 seconds @00:55:51.20 AS.reconfigure ASconfig @00:55:51.25 AS.seek 1.000ml @00:56:20.70 delay 1 second @00:56:21.71 AFV.select :holdingCoil @00:56:21.81 delay 1 second @00:56:22.82 ATV.dial :waste @00:56:24.07 AS.reconfigure ASnorm @00:56:24.12 AS.seek empty @00:57:15.61 AFV.select :carrierSolution @00:57:15.72 delay 2 seconds @00:57:17.73 AS.reconfigure ASconfig @00:57:17.78 AS.seek 0.500ml @00:57:47.28 delay 1 second @00:57:48.29 AFV.select :holdingCoil @00:57:48.39 Building Slug @00:57:48.40 ATV.dial :ARV @00:57:49.65 delay 2 seconds @00:57:51.66 ARV.dial :air1 @00:57:52.00 delay 2 seconds @00:57:54.06 AS.reconfigure ASslow2 @00:57:54.11 AS.seek 0.506ml @00:58:00.51 delay 2 seconds @00:58:02.52 ARV.dial :MM1 @00:58:03.12 delay 2 seconds @00:58:05.13 Adding MM1 @00:58:05.20 AS.seek 0.524ml @00:58:24.03 delay 25 seconds @00:58:49.04 ARV.dial :PP2 @00:58:49.39 Adding PP2 @00:58:49.41 delay 2 seconds @00:58:51.46 AS.seek 0.530ml @00:58:58.11 delay 10 seconds @00:59:08.12 ARV.dial :air2 @00:59:08.54 adding ARV air @00:59:08.56 delay 2 seconds @00:59:10.61 AS.seek 0.536ml @00:59:17.21 delay 5 seconds @00:59:22.21 ARV.dial :carrier @00:59:23.26 delay 7 seconds @00:59:30.32 AS.seek 0.594ml @01:00:29.79 delay 5 seconds @01:00:34.80 ATV.dial :ASV @01:00:35.13 delay 2 seconds @01:00:37.19 AS.seek 0.600ml @01:00:43.63 delay 4 seconds @01:00:47.64 ATV.dial ARV @01:00:47.98 delay 2 seconds @01:00:49.99 ARV.dial :carrier @01:00:50.00 delay 2 seconds @01:00:52.07 AS.seek 0.650ml @01:01:43.24 delay 4 seconds @01:01:47.25 ATV.dial PCR @01:01:47.77 ASV.dial :waste1 @01:01:47.78 ARV.dial :air1 @01:01:48.13 Positioning Slug by 0.324ml @01:01:48.21 AS.seek 0.326ml @01:07:18.89 delay 5 seconds @01:07:23.90 ATV.dial ARV @01:07:24.96 ASV.dial :PCRlock @01:07:25.54 delay 5 seconds @01:07:30.55 Running IPCap Profile59C thermocycling @01:07:30.56 PCR.program Profile59C @01:07:30.68 Profile59C should complete in < 1:39:08 @01:07:30.69 PCR.run 2:08:55 @02:33:10.54 PCR.off @02:33:11.07 PCR for IPCap completed -- closed /var/log/esp/IPCap14jan2400h.pcr @02:33:11.14 Cleaning PCR @02:33:11.15 AFV.select :carrierSolution @02:33:11.25 delay 2 seconds @02:33:13.26 AS.reconfigure ASconfig @02:33:13.31 AS.seek 0.500ml @02:33:23.75 delay 1 second @02:33:24.76 AFV.select :holdingCoil @02:33:24.86 ASV.dial :waste1 @02:33:25.22 ATV.dial :air3 @02:33:26.16 AS.reconfigure ASslow1 @02:33:26.21 AS.seek 0.510ml @02:33:28.50 delay 2 seconds @02:33:30.51 ATV.dial :bleach @02:33:30.90 AS.seek 0.810ml @02:34:32.31 delay 3 seconds @02:34:35.32 ATV.dial :air3 @02:34:35.75 delay 3 seconds @02:34:38.81 AS.seek 0.835ml @02:34:44.17 delay 3 seconds @02:34:47.18 ATV.dial :bleach @02:34:47.57 AS.seek 0.820ml @02:34:50.89 delay 3 seconds @02:34:53.90 ARV.dial :waste @02:34:54.41 delay 3 seconds @02:34:57.42 ATV.dial :ARV @02:34:58.15 AS.reconfigure ASconfig @02:34:58.20 AS.seek 0.470ml @02:35:19.00 AFV.select :carrierSolution @02:35:19.10 delay 2 seconds @02:35:21.11 AS.seek 1.000ml @02:35:52.36 delay 1 second @02:35:53.37 AFV.select :holdingCoil @02:35:53.47 delay 1 second @02:35:54.48 AS.reconfigure ASfast @02:35:54.53 AS.seek 0.000ml @02:36:22.25 AFV.select :carrierSolution @02:36:22.36 delay 2 seconds @02:36:24.92 AS.reconfigure ASconfig @02:36:24.97 AS.seek 1.000ml @02:37:23.57 delay 1 second @02:37:24.58 AFV.select :holdingCoil @02:37:24.68 delay 1 second @02:37:25.69 AS.reconfigure ASfast @02:37:25.82 AS.seek 0.000ml @02:37:53.54 AFV.select :carrierSolution @02:37:53.65 delay 2 seconds @02:37:55.66 AS.reconfigure ASconfig @02:37:55.71 AS.seek 1.000ml @02:38:54.33 delay 1 second @02:38:55.34 AFV.select :holdingCoil @02:38:55.45 delay 1 second @02:38:56.46 AS.reconfigure ASfast @02:38:56.51 AS.seek 0.000ml @02:39:24.24 ARV.dial between carrier and air1 @02:39:24.66 ATV.dial :air3 @02:39:25.60 AS.reconfigure ASslow1 @02:39:25.65 AS.seek 0.010ml @02:39:27.93 delay 2 seconds @02:39:29.94 ATV.dial :bleach @02:39:30.32 AS.seek 0.810ml @02:42:13.78 delay 3 seconds @02:42:16.79 ATV.dial :air3 @02:42:17.12 delay 3 seconds @02:42:20.18 AS.seek 0.835ml @02:42:25.59 delay 3 seconds @02:42:28.60 ATV.dial :bleach @02:42:28.99 AS.seek 0.820ml @02:42:32.30 delay 3 seconds @02:42:35.31 ATV.dial PCR @02:42:35.65 AS.reconfigure ASconfig @02:42:35.69 AS.seek empty @02:43:23.79 PCR.program :bleachProfile @02:43:23.85 PCR program should complete in < 2:45 @02:43:23.86 PCR.run 10:00 @02:46:22.06 PCR.off @02:46:22.09 AFV.select :carrierSolution @02:46:22.19 delay 2 seconds @02:46:24.20 AS.seek 1.000ml @02:47:22.86 delay 1 second @02:47:23.87 AFV.select :holdingCoil @02:47:23.97 delay 1 second @02:47:24.98 AS.reconfigure ASfast @02:47:25.03 AS.seek 0.000ml @02:47:52.77 delay 3 seconds @02:47:55.78 AFV.select :carrierSolution @02:47:55.88 delay 2 seconds @02:47:57.89 AS.reconfigure ASconfig @02:47:57.94 AS.seek 1.000ml @02:48:56.56 delay 1 second @02:48:57.57 AFV.select :holdingCoil @02:48:57.67 delay 1 second @02:48:58.68 AS.reconfigure ASfast @02:48:58.73 AS.seek 0.000ml @02:49:26.45 delay 3 seconds @02:49:29.46 AFV.select :carrierSolution @02:49:29.56 delay 2 seconds @02:49:31.57 AS.reconfigure ASconfig @02:49:31.62 AS.seek 1.000ml @02:50:30.24 delay 1 second @02:50:31.25 AFV.select :holdingCoil @02:50:31.36 delay 1 second @02:50:32.37 AS.reconfigure ASfast @02:50:32.42 AS.seek 0.000ml @02:51:00.13 delay 3 seconds @02:51:03.14 AFV.select :carrierSolution @02:51:03.24 delay 2 seconds @02:51:05.25 AS.reconfigure ASconfig @02:51:05.30 AS.seek 1.000ml @02:52:03.93 delay 1 second @02:52:04.94 AFV.select :holdingCoil @02:52:05.04 delay 1 second @02:52:06.05 AS.reconfigure ASfast @02:52:06.10 AS.seek 0.000ml @02:52:33.83 delay 3 seconds @02:52:36.84 AFV.select :carrierSolution @02:52:36.95 delay 2 seconds @02:52:38.96 AS.reconfigure ASconfig @02:52:39.01 AS.seek 1.000ml @02:53:37.60 delay 1 second @02:53:38.61 AFV.select :holdingCoil @02:53:38.71 delay 1 second @02:53:39.72 AS.reconfigure ASnorm @02:53:39.77 AS.seek 0.000ml @02:54:31.25 Setting up PCR for IPCem -- writing /var/log/esp/IPCem14jan2402h.pcr @02:54:31.26 Building PCR Slug from ASV with MM3 mix, PP5 primer @02:54:31.28 Priming with carrier @02:54:31.29 ATV.dial ARV @02:54:31.81 ARV.dial :carrier @02:54:32.11 AS.seek 0.500ml @02:54:57.98 AFV.select :carrierSolution @02:54:58.09 delay 2 seconds @02:55:00.10 AS.reconfigure ASconfig @02:55:00.15 AS.seek 1.000ml @02:55:29.62 delay 1 second @02:55:30.63 AFV.select :holdingCoil @02:55:30.88 delay 1 second @02:55:31.89 ATV.dial :waste @02:55:33.15 AS.reconfigure ASnorm @02:55:33.20 AS.seek empty @02:56:24.70 AFV.select :carrierSolution @02:56:24.81 delay 2 seconds @02:56:26.82 AS.reconfigure ASconfig @02:56:26.87 AS.seek 0.500ml @02:56:56.37 delay 1 second @02:56:57.38 AFV.select :holdingCoil @02:56:57.48 Building Slug @02:56:57.49 ATV.dial :ARV @02:56:58.75 delay 2 seconds @02:57:00.76 ARV.dial :air1 @02:57:01.10 delay 2 seconds @02:57:03.16 AS.reconfigure ASslow2 @02:57:03.21 AS.seek 0.506ml @02:57:09.67 delay 2 seconds @02:57:11.74 ARV.dial :MM3 @02:57:12.62 delay 2 seconds @02:57:14.63 Adding MM3 @02:57:14.70 AS.seek 0.524ml @02:57:33.29 delay 25 seconds @02:57:58.30 ARV.dial :PP5 @02:57:58.64 Adding PP5 @02:57:58.65 delay 2 seconds @02:58:00.71 AS.seek 0.530ml @02:58:06.96 delay 10 seconds @02:58:16.97 ARV.dial :air3 @02:58:18.02 adding ARV air @02:58:18.04 delay 2 seconds @02:58:20.09 AS.seek 0.536ml @02:58:26.32 delay 5 seconds @02:58:31.33 ARV.dial :carrier @02:58:32.37 delay 7 seconds @02:58:39.43 AS.seek 0.594ml @02:59:38.82 delay 5 seconds @02:59:43.83 ATV.dial :ASV @02:59:44.16 delay 2 seconds @02:59:46.22 AS.seek 0.600ml @02:59:52.47 delay 4 seconds @02:59:56.48 ATV.dial ARV @02:59:56.82 delay 2 seconds @02:59:58.83 ARV.dial :carrier @02:59:58.84 delay 2 seconds @03:00:00.91 AS.seek 0.650ml @03:00:52.02 delay 4 seconds @03:00:56.03 ATV.dial PCR @03:00:56.53 ASV.dial :waste1 @03:00:56.54 ARV.dial :air1 @03:00:56.90 Positioning Slug by 0.324ml @03:00:56.97 AS.seek 0.326ml @03:06:27.63 delay 5 seconds @03:06:32.64 ATV.dial ARV @03:06:33.27 ASV.dial :PCRlock @03:06:34.05 delay 5 seconds @03:06:39.06 Running IPCem ProfileEM59C thermocycling @03:06:39.07 PCR.program ProfileEM59C @03:06:39.20 ProfileEM59C should complete in < 2:11:38 @03:06:39.21 PCR.run 2:49:32.5 @05:05:03.90 PCR.off @05:05:04.44 PCR for IPCem completed -- closed /var/log/esp/IPCem14jan2402h.pcr @05:05:04.45 Full MFB Decontamination @05:05:04.46 Decon extraction column and decon AIV @05:05:04.48 SPE.hold 2:46:40,55.0C @05:05:04.50 ASV.dial :SPEdelivery @05:05:05.06 delay 20 seconds @05:05:25.07 AFV.select :carrierSolution @05:05:25.17 delay 2 seconds @05:05:27.18 AS.reconfigure ASconfig @05:05:27.22 AS.seek 0.500ml @05:05:38.19 delay 1 second @05:05:39.20 AFV.select :holdingCoil @05:05:39.30 ATV.dial :air1 @05:05:40.84 AS.reconfigure ASslow1 @05:05:40.89 AS.seek 0.520ml @05:05:45.25 ATV.dial :ASV @05:05:46.54 ASV.dial :SPErecovery @05:05:47.39 AS.seek 0.620ml @05:06:08.06 ATV.dial :waste @05:06:09.54 AS.reconfigure ASnorm @05:06:09.59 AS.seek empty @05:06:41.62 AFV.select :holdingCoil @05:06:41.63 ATV.dial :waste @05:06:41.65 AS.reconfigure ASconfig @05:06:41.71 AS.seek 0.000ml @05:06:42.04 AFV.select :carrierSolution @05:06:42.15 delay 2 seconds @05:06:44.16 AS.seek 0.500ml @05:07:13.59 delay 1 second @05:07:14.60 AFV.select :holdingCoil @05:07:14.70 ATV.dial :air3 @05:07:15.28 AS.reconfigure ASslow1 @05:07:15.33 AS.seek 0.520ml @05:07:19.67 ATV.dial :air3 @05:07:19.75 AS.seek 0.530ml @05:07:22.06 delay 2 seconds @05:07:24.07 ATV.dial :bleach @05:07:24.47 AS.seek 0.710ml @05:08:01.45 delay 3 seconds @05:08:04.46 ATV.dial :air3 @05:08:04.90 delay 3 seconds @05:08:07.96 AS.seek 0.735ml @05:08:13.34 delay 3 seconds @05:08:16.35 ATV.dial :bleach @05:08:16.75 AS.seek 0.720ml @05:08:20.07 delay 3 seconds @05:08:23.08 ATV.dial ASV @05:08:24.07 ASV.dial :SPErecovery @05:08:24.15 AS.seek 0.365ml @05:09:36.80 ATV.dial :waste @05:09:38.27 AS.reconfigure ASnorm @05:09:38.32 AS.seek empty @05:09:57.33 ATV.dial :air3 @05:09:57.99 AS.reconfigure ASslow1 @05:09:58.04 AS.seek 0.009ml @05:10:00.42 delay 2 seconds @05:10:02.42 ATV.dial :bleach @05:10:02.80 AS.seek 0.579ml @05:11:59.28 delay 3 seconds @05:12:02.29 ATV.dial :air3 @05:12:02.73 delay 3 seconds @05:12:05.79 AS.seek 0.604ml @05:12:11.17 delay 3 seconds @05:12:14.18 ATV.dial :bleach @05:12:14.66 AS.seek 0.589ml @05:12:17.98 delay 3 seconds @05:12:20.99 ATV.dial :ASV @05:12:21.87 ASV.dial :wasteR @05:12:22.49 AS.seek 0.000ml @05:14:22.87 delay 1:00 @05:15:22.88 ATV.dial ASV @05:15:22.89 AFV.select :carrierSolution @05:15:23.00 delay 2 seconds @05:15:25.01 AS.reconfigure ASconfig @05:15:25.06 AS.seek 1.000ml @05:16:23.66 delay 1 second @05:16:24.67 AFV.select :holdingCoil @05:16:24.77 delay 1 second @05:16:25.78 ASV.dial :wasteR @05:16:25.87 AS.reconfigure ASslow1 @05:16:25.92 AS.seek 0.000ml @05:19:50.19 delay 10 seconds @05:20:00.20 ATV.dial :air3 @05:20:01.41 AS.seek 0.010ml @05:20:03.75 delay 2 seconds @05:20:05.76 ATV.dial :bleach @05:20:06.14 AS.seek 0.580ml @05:22:02.65 delay 3 seconds @05:22:05.66 ATV.dial :air3 @05:22:06.08 delay 3 seconds @05:22:09.14 AS.seek 0.605ml @05:22:14.52 delay 3 seconds @05:22:17.53 ATV.dial :bleach @05:22:17.91 AS.seek 0.590ml @05:22:21.21 delay 3 seconds @05:22:24.22 ATV.dial ASV @05:22:25.10 ASV.dial :SPEdelivery @05:22:25.59 AS.seek 0.000ml @05:24:26.19 delay 1:00 @05:25:26.20 AFV.select :carrierSolution @05:25:26.30 delay 2 seconds @05:25:28.31 AS.reconfigure ASconfig @05:25:28.36 AS.seek 1.000ml @05:26:26.96 delay 1 second @05:26:27.97 AFV.select :holdingCoil @05:26:28.07 delay 1 second @05:26:29.08 ATV.dial ASV @05:26:29.09 ASV.dial :SPEdelivery @05:26:29.11 AS.reconfigure ASslow1 @05:26:29.16 AS.seek 0.000ml @05:29:53.60 delay 10 seconds @05:30:03.61 ATV.dial :air2 @05:30:04.33 AS.reconfigure ASslow0 @05:30:04.38 AS.seek 0.800ml @05:31:26.27 delay 3 seconds @05:31:29.28 ATV.dial :ASV @05:31:29.99 ASV.dial :SPEdelivery @05:31:30.01 AS.reconfigure ASslow1 @05:31:30.06 AS.seek 0.030ml @05:34:07.36 delay 20 seconds @05:34:27.37 ATV.dial :waste,via => air3 @05:34:28.79 AS.reconfigure ASconfig @05:34:28.84 AS.seek empty @05:34:30.92 SPE.coast @05:34:31.01 Removing Bleach from Mixing Coil @05:34:31.02 AFV.select :carrierSolution @05:34:31.13 delay 2 seconds @05:34:33.14 AS.seek 0.500ml @05:35:02.62 delay 1 second @05:35:03.63 AFV.select :holdingCoil @05:35:03.73 ATV.dial :ASV @05:35:05.88 ASV.dial :SPErecovery @05:35:06.58 AS.reconfigure ASslow1 @05:35:06.63 AS.seek 0.730ml @05:35:53.82 ASV.dial :wasteD @05:35:54.35 AS.reconfigure ASnorm @05:35:54.40 AS.seek 0.000ml @05:36:32.04 AFV.select :carrierSolution @05:36:32.15 delay 2 seconds @05:36:34.16 AS.reconfigure ASconfig @05:36:34.21 AS.seek 0.500ml @05:37:03.66 delay 1 second @05:37:04.67 AFV.select :holdingCoil @05:37:04.78 ATV.dial ASV @05:37:04.79 ASV.dial :SPErecovery @05:37:05.51 AS.reconfigure ASslow0 @05:37:05.56 AS.seek 0.300ml @05:37:26.27 ATV.dial :air2 @05:37:27.02 AS.reconfigure ASslow1 @05:37:27.07 AS.seek 0.320ml @05:37:31.43 ATV.dial ASV @05:37:32.29 AS.reconfigure ASslow0 @05:37:32.34 AS.seek 0.570ml @05:37:58.13 ASV.dial :wasteD @05:37:59.08 AS.reconfigure ASnorm @05:37:59.13 AS.seek 0.000ml @05:38:28.61 AFV.select :carrierSolution @05:38:28.72 delay 2 seconds @05:38:30.74 AS.reconfigure ASconfig @05:38:30.79 AS.seek 0.500ml @05:39:00.25 delay 1 second @05:39:01.26 AFV.select :holdingCoil @05:39:01.36 ATV.dial ASV @05:39:01.37 ASV.dial :SPErecovery @05:39:01.92 AS.reconfigure ASslow0 @05:39:01.97 AS.seek 0.300ml @05:39:22.68 ATV.dial :air2 @05:39:23.44 AS.reconfigure ASslow1 @05:39:23.49 AS.seek 0.320ml @05:39:27.87 ATV.dial ASV @05:39:28.64 AS.reconfigure ASslow0 @05:39:28.69 AS.seek 0.570ml @05:39:54.54 ASV.dial :wasteD @05:39:55.21 AS.reconfigure ASnorm @05:39:55.26 AS.seek 0.000ml @05:40:24.74 ATV.dial :air2 @05:40:25.46 AS.reconfigure ASslow0 @05:40:25.51 AS.seek 0.300ml @05:40:56.48 ATV.dial :ASV @05:40:57.20 ASV.dial :wasteD @05:40:57.22 AS.reconfigure ASconfig @05:40:57.27 AS.seek 0.050ml @05:41:12.27 ATV.dial :waste @05:41:13.69 AS.seek 0.000ml @05:41:16.90 ASV.dial :SPEdelivery @05:41:17.91 deconLysateINT @05:41:17.92 ATV.dial :air3 @05:41:18.49 AS.reconfigure ASslow1 @05:41:18.54 AS.seek 0.010ml @05:41:20.90 delay 2 seconds @05:41:22.91 ATV.dial :bleach @05:41:23.31 AS.seek 0.960ml @05:44:37.31 delay 3 seconds @05:44:40.32 ATV.dial :air3 @05:44:40.75 delay 3 seconds @05:44:43.81 AS.seek 0.985ml @05:44:49.16 delay 3 seconds @05:44:52.17 ATV.dial :bleach @05:44:52.55 AS.seek 0.970ml @05:44:55.86 delay 3 seconds @05:44:58.87 ATV.dial :AIV @05:44:59.94 AIV.dial :coilToMFB @05:44:59.95 AWV.select :waste @05:44:59.97 AFV.select :carrierSolution @05:45:00.07 delay 2 seconds @05:45:02.08 AS.reconfigure ASconfig @05:45:02.13 AS.seek 1.000ml @05:45:04.23 delay 1 second @05:45:05.24 AFV.select :holdingCoil @05:45:05.34 delay 1 second @05:45:06.35 AS.reconfigure ASslow0 @05:45:06.40 AS.seek empty @05:46:48.85 AFV.select :carrierSolution @05:46:48.96 delay 2 seconds @05:46:50.97 AS.reconfigure ASconfig @05:46:51.02 AS.seek 1.000ml @05:47:49.63 delay 1 second @05:47:50.64 AFV.select :holdingCoil @05:47:50.74 delay 1 second @05:47:51.75 AS.reconfigure ASslow0 @05:47:51.80 AS.seek empty @05:49:34.24 AFV.select :carrierSolution @05:49:34.35 delay 2 seconds @05:49:36.36 AS.reconfigure ASconfig @05:49:36.41 AS.seek 1.000ml @05:50:35.10 delay 1 second @05:50:36.11 AFV.select :holdingCoil @05:50:36.21 delay 1 second @05:50:37.22 AS.reconfigure ASslow0 @05:50:37.27 AS.seek empty @05:52:19.69 ATV.dial :air2 @05:52:20.27 AS.reconfigure ASslow1 @05:52:20.32 AS.seek 0.009ml @05:52:22.67 AS.reconfigure ASslow0 @05:52:22.72 AS.seek 1.000ml @05:54:04.06 ATV.dial AIV @05:54:04.60 AS.seek 0.020ml @05:55:44.91 ATV.dial :waste @05:55:46.18 AS.seek 0.000ml @05:55:48.50 AWV.select :air @05:55:48.65 Cleaning PCR @05:55:48.66 AFV.select :carrierSolution @05:55:48.76 delay 2 seconds @05:55:50.77 AS.reconfigure ASconfig @05:55:50.82 AS.seek 0.500ml @05:56:20.25 delay 1 second @05:56:21.26 AFV.select :holdingCoil @05:56:21.36 ASV.dial :waste1 @05:56:21.95 ATV.dial :air3 @05:56:22.51 AS.reconfigure ASslow1 @05:56:23.08 AS.seek 0.510ml @05:56:25.39 delay 2 seconds @05:56:27.40 ATV.dial :bleach @05:56:27.79 AS.seek 0.810ml @05:57:29.16 delay 3 seconds @05:57:32.17 ATV.dial :air3 @05:57:32.51 delay 3 seconds @05:57:35.57 AS.seek 0.835ml @05:57:40.97 delay 3 seconds @05:57:43.98 ATV.dial :bleach @05:57:44.36 AS.seek 0.820ml @05:57:47.69 delay 3 seconds @05:57:50.70 ARV.dial :waste @05:57:51.20 delay 3 seconds @05:57:54.21 ATV.dial :ARV @05:57:54.95 AS.reconfigure ASconfig @05:57:55.00 AS.seek 0.470ml @05:58:15.68 AFV.select :carrierSolution @05:58:15.79 delay 2 seconds @05:58:17.80 AS.seek 1.000ml @05:58:49.01 delay 1 second @05:58:50.02 AFV.select :holdingCoil @05:58:50.13 delay 1 second @05:58:51.14 AS.reconfigure ASfast @05:58:51.19 AS.seek 0.000ml @05:59:18.92 AFV.select :carrierSolution @05:59:19.03 delay 2 seconds @05:59:21.04 AS.reconfigure ASconfig @05:59:21.09 AS.seek 1.000ml @06:00:19.71 delay 1 second @06:00:20.72 AFV.select :holdingCoil @06:00:20.85 delay 1 second @06:00:21.86 AS.reconfigure ASfast @06:00:21.91 AS.seek 0.000ml @06:00:49.65 AFV.select :carrierSolution @06:00:49.76 delay 2 seconds @06:00:51.77 AS.reconfigure ASconfig @06:00:51.82 AS.seek 1.000ml @06:01:50.41 delay 1 second @06:01:51.42 AFV.select :holdingCoil @06:01:51.52 delay 1 second @06:01:52.53 AS.reconfigure ASfast @06:01:52.58 AS.seek 0.000ml @06:02:20.30 ARV.dial between carrier and air1 @06:02:20.72 ATV.dial :air3 @06:02:21.66 AS.reconfigure ASslow1 @06:02:21.71 AS.seek 0.010ml @06:02:24.05 delay 2 seconds @06:02:26.06 ATV.dial :bleach @06:02:26.45 AS.seek 0.810ml @06:05:09.86 delay 3 seconds @06:05:12.87 ATV.dial :air3 @06:05:13.30 delay 3 seconds @06:05:16.36 AS.seek 0.835ml @06:05:21.70 delay 3 seconds @06:05:24.71 ATV.dial :bleach @06:05:25.10 AS.seek 0.820ml @06:05:28.42 delay 3 seconds @06:05:31.43 ATV.dial PCR @06:05:31.77 AS.reconfigure ASconfig @06:05:31.81 AS.seek empty @06:06:19.95 PCR.program :bleachProfile @06:06:20.01 PCR program should complete in < 2:45 @06:06:20.02 PCR.run 10:00 @06:09:21.24 PCR.off @06:09:21.27 AFV.select :carrierSolution @06:09:21.37 delay 2 seconds @06:09:23.38 AS.seek 1.000ml @06:10:22.05 delay 1 second @06:10:23.06 AFV.select :holdingCoil @06:10:23.16 delay 1 second @06:10:24.17 AS.reconfigure ASfast @06:10:24.22 AS.seek 0.000ml @06:10:51.96 delay 3 seconds @06:10:54.97 AFV.select :carrierSolution @06:10:55.08 delay 2 seconds @06:10:57.09 AS.reconfigure ASconfig @06:10:57.14 AS.seek 1.000ml @06:11:55.75 delay 1 second @06:11:56.76 AFV.select :holdingCoil @06:11:56.87 delay 1 second @06:11:57.88 AS.reconfigure ASfast @06:11:57.93 AS.seek 0.000ml @06:12:25.69 delay 3 seconds @06:12:28.70 AFV.select :carrierSolution @06:12:28.80 delay 2 seconds @06:12:30.81 AS.reconfigure ASconfig @06:12:30.86 AS.seek 1.000ml @06:13:29.49 delay 1 second @06:13:30.50 AFV.select :holdingCoil @06:13:30.61 delay 1 second @06:13:31.62 AS.reconfigure ASfast @06:13:31.67 AS.seek 0.000ml @06:13:59.40 delay 3 seconds @06:14:02.41 AFV.select :carrierSolution @06:14:02.51 delay 2 seconds @06:14:04.52 AS.reconfigure ASconfig @06:14:04.57 AS.seek 1.000ml @06:15:03.18 delay 1 second @06:15:04.19 AFV.select :holdingCoil @06:15:04.30 delay 1 second @06:15:05.31 AS.reconfigure ASfast @06:15:05.35 AS.seek 0.000ml @06:15:33.07 delay 3 seconds @06:15:36.08 AFV.select :carrierSolution @06:15:36.18 delay 2 seconds @06:15:38.19 AS.reconfigure ASconfig @06:15:38.24 AS.seek 1.000ml @06:16:36.84 delay 1 second @06:16:37.85 AFV.select :holdingCoil @06:16:38.00 delay 1 second @06:16:39.01 AS.reconfigure ASnorm @06:16:39.06 AS.seek 0.000ml @06:17:30.53 AFV.select :carrierSolution @06:17:30.63 delay 2 seconds @06:17:32.64 AS.reconfigure ASconfig @06:17:32.69 AS.seek 1.000ml @06:18:31.29 delay 1 second @06:18:32.30 AFV.select :holdingCoil @06:18:32.40 delay 1 second @06:18:33.41 ATV.dial :waste @06:18:34.41 AS.reconfigure ASnorm @06:18:34.46 AS.seek 0.500ml @06:19:00.40 ATV.dial :waste2 @06:19:01.47 AS.seek 0.000ml @06:19:27.35 Setting up PCR for HF183em -- writing /var/log/esp/HF183em14jan2406h.pcr @06:19:27.36 Building PCR Slug from elutionWater with MM2 mix, PP4 primer @06:19:27.88 Priming with carrier @06:19:27.91 ATV.dial ARV @06:19:28.62 ARV.dial :carrier @06:19:28.92 AS.seek 0.500ml @06:19:54.84 AFV.select :carrierSolution @06:19:54.94 delay 2 seconds @06:19:56.95 AS.reconfigure ASconfig @06:19:57.00 AS.seek 1.000ml @06:20:26.43 delay 1 second @06:20:27.44 AFV.select :holdingCoil @06:20:27.54 delay 1 second @06:20:28.55 ATV.dial :waste @06:20:29.82 AS.reconfigure ASnorm @06:20:29.87 AS.seek empty @06:21:21.37 AFV.select :carrierSolution @06:21:21.48 delay 2 seconds @06:21:23.49 AS.reconfigure ASconfig @06:21:23.54 AS.seek 0.500ml @06:21:53.04 delay 1 second @06:21:54.05 AFV.select :holdingCoil @06:21:54.15 Building Slug @06:21:54.16 ATV.dial :ARV @06:21:55.41 delay 2 seconds @06:21:57.42 ARV.dial :air1 @06:21:57.76 delay 2 seconds @06:21:59.82 AS.reconfigure ASslow2 @06:21:59.87 AS.seek 0.506ml @06:22:06.18 delay 2 seconds @06:22:08.19 ARV.dial :MM2 @06:22:09.43 delay 2 seconds @06:22:11.44 Adding MM2 @06:22:11.50 AS.seek 0.524ml @06:22:30.22 delay 25 seconds @06:22:55.23 ARV.dial :PP4 @06:22:55.67 Adding PP4 @06:22:55.68 delay 2 seconds @06:22:57.74 AS.seek 0.530ml @06:23:04.10 delay 10 seconds @06:23:14.11 ARV.dial :air3 @06:23:14.47 adding ARV air @06:23:14.48 delay 2 seconds @06:23:16.54 AS.seek 0.536ml @06:23:23.03 delay 5 seconds @06:23:28.04 ARV.dial :carrier @06:23:29.08 delay 7 seconds @06:23:36.14 AS.seek 0.594ml @06:24:35.53 delay 5 seconds @06:24:40.54 ATV.dial :elutionWater @06:24:41.61 delay 2 seconds @06:24:43.67 AS.seek 0.600ml @06:24:49.92 delay 4 seconds @06:24:53.93 ATV.dial ARV @06:24:54.98 delay 2 seconds @06:24:56.99 ARV.dial :carrier @06:24:57.00 delay 2 seconds @06:24:59.06 AS.seek 0.650ml @06:25:50.28 delay 4 seconds @06:25:54.29 ATV.dial PCR @06:25:54.81 ASV.dial :waste1 @06:25:54.82 ARV.dial :air1 @06:25:55.16 Positioning Slug by 0.324ml @06:25:55.23 AS.seek 0.326ml @06:31:25.95 delay 5 seconds @06:31:30.96 ATV.dial ARV @06:31:31.59 ASV.dial :PCRlock @06:31:32.09 delay 5 seconds @06:31:37.10 Running HF183em ProfileEM60C2 thermocycling @06:31:37.11 PCR.program ProfileEM60C2 @06:31:37.24 ProfileEM60C2 should complete in < 2:11:38 @06:31:37.25 PCR.run 2:49:32.5 @08:29:05.38 PCR.off @08:29:05.92 PCR for HF183em completed -- closed /var/log/esp/HF183em14jan2406h.pcr @08:29:05.98 Cleaning PCR @08:29:05.99 AFV.select :carrierSolution @08:29:06.10 delay 2 seconds @08:29:08.11 AS.reconfigure ASconfig @08:29:08.16 AS.seek 0.500ml @08:29:18.59 delay 1 second @08:29:19.60 AFV.select :holdingCoil @08:29:19.70 ASV.dial :waste1 @08:29:20.45 ATV.dial :air3 @08:29:21.37 AS.reconfigure ASslow1 @08:29:21.42 AS.seek 0.510ml @08:29:23.70 delay 2 seconds @08:29:25.71 ATV.dial :bleach @08:29:26.09 AS.seek 0.810ml @08:30:27.51 delay 3 seconds @08:30:30.52 ATV.dial :air3 @08:30:30.95 delay 3 seconds @08:30:34.01 AS.seek 0.835ml @08:30:39.35 delay 3 seconds @08:30:42.36 ATV.dial :bleach @08:30:42.74 AS.seek 0.820ml @08:30:46.07 delay 3 seconds @08:30:49.08 ARV.dial :waste @08:30:49.59 delay 3 seconds @08:30:52.60 ATV.dial :ARV @08:30:53.34 AS.reconfigure ASconfig @08:30:53.39 AS.seek 0.470ml @08:31:14.22 AFV.select :carrierSolution @08:31:14.33 delay 2 seconds @08:31:16.34 AS.seek 1.000ml @08:31:47.52 delay 1 second @08:31:48.53 AFV.select :holdingCoil @08:31:48.63 delay 1 second @08:31:49.64 AS.reconfigure ASfast @08:31:49.69 AS.seek 0.000ml @08:32:17.41 AFV.select :carrierSolution @08:32:17.52 delay 2 seconds @08:32:19.53 AS.reconfigure ASconfig @08:32:19.58 AS.seek 1.000ml @08:33:18.19 delay 1 second @08:33:19.20 AFV.select :holdingCoil @08:33:19.30 delay 1 second @08:33:20.31 AS.reconfigure ASfast @08:33:20.36 AS.seek 0.000ml @08:33:48.08 AFV.select :carrierSolution @08:33:48.19 delay 2 seconds @08:33:50.20 AS.reconfigure ASconfig @08:33:50.24 AS.seek 1.000ml @08:34:48.86 delay 1 second @08:34:49.87 AFV.select :holdingCoil @08:34:49.98 delay 1 second @08:34:50.99 AS.reconfigure ASfast @08:34:51.04 AS.seek 0.000ml @08:35:18.76 ARV.dial between carrier and air1 @08:35:19.19 ATV.dial :air3 @08:35:20.14 AS.reconfigure ASslow1 @08:35:20.19 AS.seek 0.010ml @08:35:22.51 delay 2 seconds @08:35:24.52 ATV.dial :bleach @08:35:24.91 AS.seek 0.810ml @08:38:08.32 delay 3 seconds @08:38:11.33 ATV.dial :air3 @08:38:11.66 delay 3 seconds @08:38:14.72 AS.seek 0.835ml @08:38:20.09 delay 3 seconds @08:38:23.10 ATV.dial :bleach @08:38:23.48 AS.seek 0.820ml @08:38:26.80 delay 3 seconds @08:38:29.81 ATV.dial PCR @08:38:30.16 AS.reconfigure ASconfig @08:38:30.20 AS.seek empty @08:39:18.33 PCR.program :bleachProfile @08:39:18.39 PCR program should complete in < 2:45 @08:39:18.40 PCR.run 10:00 @08:42:16.60 PCR.off @08:42:16.64 AFV.select :carrierSolution @08:42:16.74 delay 2 seconds @08:42:18.75 AS.seek 1.000ml @08:43:17.40 delay 1 second @08:43:18.41 AFV.select :holdingCoil @08:43:18.51 delay 1 second @08:43:19.52 AS.reconfigure ASfast @08:43:19.57 AS.seek 0.000ml @08:43:47.29 delay 3 seconds @08:43:50.30 AFV.select :carrierSolution @08:43:50.41 delay 2 seconds @08:43:52.42 AS.reconfigure ASconfig @08:43:52.47 AS.seek 1.000ml @08:44:51.04 delay 1 second @08:44:52.05 AFV.select :holdingCoil @08:44:52.15 delay 1 second @08:44:53.71 AS.reconfigure ASfast @08:44:53.75 AS.seek 0.000ml @08:45:21.49 delay 3 seconds @08:45:24.50 AFV.select :carrierSolution @08:45:24.60 delay 2 seconds @08:45:26.61 AS.reconfigure ASconfig @08:45:26.66 AS.seek 1.000ml @08:46:25.28 delay 1 second @08:46:26.29 AFV.select :holdingCoil @08:46:26.39 delay 1 second @08:46:27.40 AS.reconfigure ASfast @08:46:27.45 AS.seek 0.000ml @08:46:55.17 delay 3 seconds @08:46:58.18 AFV.select :carrierSolution @08:46:58.28 delay 2 seconds @08:47:00.29 AS.reconfigure ASconfig @08:47:00.34 AS.seek 1.000ml @08:47:58.95 delay 1 second @08:47:59.96 AFV.select :holdingCoil @08:48:00.07 delay 1 second @08:48:01.08 AS.reconfigure ASfast @08:48:01.13 AS.seek 0.000ml @08:48:28.86 delay 3 seconds @08:48:31.87 AFV.select :carrierSolution @08:48:31.98 delay 2 seconds @08:48:33.99 AS.reconfigure ASconfig @08:48:34.03 AS.seek 1.000ml @08:49:32.65 delay 1 second @08:49:33.66 AFV.select :holdingCoil @08:49:33.77 delay 1 second @08:49:34.78 AS.reconfigure ASnorm @08:49:34.83 AS.seek 0.000ml @08:50:26.32 Setting up PCR for ENTap -- writing /var/log/esp/ENTap14jan2408h.pcr @08:50:26.33 Building PCR Slug from elutionWater with MM1 mix, PP1 primer @08:50:26.34 Priming with carrier @08:50:26.37 ATV.dial ARV @08:50:26.88 ARV.dial :carrier @08:50:27.18 AS.seek 0.500ml @08:50:53.07 AFV.select :carrierSolution @08:50:53.18 delay 2 seconds @08:50:55.19 AS.reconfigure ASconfig @08:50:55.24 AS.seek 1.000ml @08:51:24.69 delay 1 second @08:51:25.70 AFV.select :holdingCoil @08:51:25.80 delay 1 second @08:51:26.81 ATV.dial :waste @08:51:28.06 AS.reconfigure ASnorm @08:51:28.11 AS.seek empty @08:52:19.60 AFV.select :carrierSolution @08:52:19.71 delay 2 seconds @08:52:21.72 AS.reconfigure ASconfig @08:52:21.77 AS.seek 0.500ml @08:52:51.27 delay 1 second @08:52:52.28 AFV.select :holdingCoil @08:52:52.39 Building Slug @08:52:52.40 ATV.dial :ARV @08:52:53.64 delay 2 seconds @08:52:55.65 ARV.dial :air1 @08:52:55.99 delay 2 seconds @08:52:58.05 AS.reconfigure ASslow2 @08:52:58.10 AS.seek 0.506ml @08:53:04.61 delay 2 seconds @08:53:06.62 ARV.dial :MM1 @08:53:07.13 delay 2 seconds @08:53:09.14 Adding MM1 @08:53:09.21 AS.seek 0.524ml @08:53:27.97 delay 25 seconds @08:53:52.98 ARV.dial :PP1 @08:53:53.34 Adding PP1 @08:53:53.35 delay 2 seconds @08:53:55.41 AS.seek 0.530ml @08:54:01.91 delay 10 seconds @08:54:11.92 ARV.dial :air1 @08:54:12.25 adding ARV air @08:54:12.27 delay 2 seconds @08:54:14.32 AS.seek 0.536ml @08:54:20.84 delay 5 seconds @08:54:25.85 ARV.dial :carrier @08:54:26.17 delay 7 seconds @08:54:33.23 AS.seek 0.594ml @08:55:32.64 delay 5 seconds @08:55:37.65 ATV.dial :elutionWater @08:55:38.83 delay 2 seconds @08:55:40.89 AS.seek 0.600ml @08:55:47.54 delay 4 seconds @08:55:51.55 ATV.dial ARV @08:55:52.61 delay 2 seconds @08:55:54.62 ARV.dial :carrier @08:55:54.63 delay 2 seconds @08:55:56.70 AS.seek 0.650ml @08:56:47.93 delay 4 seconds @08:56:51.94 ATV.dial PCR @08:56:52.46 ASV.dial :waste1 @08:56:52.47 ARV.dial :air1 @08:56:52.83 Positioning Slug by 0.324ml @08:56:52.90 AS.seek 0.326ml @09:02:23.36 delay 5 seconds @09:02:28.37 ATV.dial ARV @09:02:28.91 ASV.dial :PCRlock @09:02:29.41 delay 5 seconds @09:02:34.42 Running ENTap Profile60C thermocycling @09:02:34.43 PCR.program Profile60C @09:02:34.55 Profile60C should complete in < 1:39:08 @09:02:34.56 PCR.run 2:08:55 @10:26:53.65 PCR.off @10:26:54.18 PCR for ENTap completed -- closed /var/log/esp/ENTap14jan2408h.pcr @10:26:54.24 Cleaning PCR @10:26:54.25 AFV.select :carrierSolution @10:26:54.36 delay 2 seconds @10:26:56.37 AS.reconfigure ASconfig @10:26:56.42 AS.seek 0.500ml @10:27:06.86 delay 1 second @10:27:07.87 AFV.select :holdingCoil @10:27:07.97 ASV.dial :waste1 @10:27:08.67 ATV.dial :air3 @10:27:09.71 AS.reconfigure ASslow1 @10:27:09.76 AS.seek 0.510ml @10:27:12.09 delay 2 seconds @10:27:14.10 ATV.dial :bleach @10:27:14.49 AS.seek 0.810ml @10:28:16.00 delay 3 seconds @10:28:19.01 ATV.dial :air3 @10:28:19.34 delay 3 seconds @10:28:22.41 AS.seek 0.835ml @10:28:27.74 delay 3 seconds @10:28:30.75 ATV.dial :bleach @10:28:31.14 AS.seek 0.820ml @10:28:34.46 delay 3 seconds @10:28:37.47 ARV.dial :waste @10:28:38.07 delay 3 seconds @10:28:41.08 ATV.dial :ARV @10:28:41.81 AS.reconfigure ASconfig @10:28:41.86 AS.seek 0.470ml @10:29:02.55 AFV.select :carrierSolution @10:29:02.66 delay 2 seconds @10:29:04.67 AS.seek 1.000ml @10:29:35.91 delay 1 second @10:29:36.92 AFV.select :holdingCoil @10:29:37.02 delay 1 second @10:29:38.03 AS.reconfigure ASfast @10:29:38.08 AS.seek 0.000ml @10:30:05.78 AFV.select :carrierSolution @10:30:05.89 delay 2 seconds @10:30:07.90 AS.reconfigure ASconfig @10:30:07.94 AS.seek 1.000ml @10:31:06.53 delay 1 second @10:31:07.54 AFV.select :holdingCoil @10:31:07.64 delay 1 second @10:31:08.65 AS.reconfigure ASfast @10:31:08.70 AS.seek 0.000ml @10:31:36.47 AFV.select :carrierSolution @10:31:36.58 delay 2 seconds @10:31:38.59 AS.reconfigure ASconfig @10:31:38.63 AS.seek 1.000ml @10:32:37.23 delay 1 second @10:32:38.24 AFV.select :holdingCoil @10:32:38.35 delay 1 second @10:32:39.36 AS.reconfigure ASfast @10:32:39.41 AS.seek 0.000ml @10:33:07.12 ARV.dial between carrier and air1 @10:33:07.54 ATV.dial :air3 @10:33:08.49 AS.reconfigure ASslow1 @10:33:08.54 AS.seek 0.010ml @10:33:10.95 delay 2 seconds @10:33:12.96 ATV.dial :bleach @10:33:13.34 AS.seek 0.810ml @10:35:56.76 delay 3 seconds @10:35:59.77 ATV.dial :air3 @10:36:00.19 delay 3 seconds @10:36:03.25 AS.seek 0.835ml @10:36:08.62 delay 3 seconds @10:36:11.63 ATV.dial :bleach @10:36:12.01 AS.seek 0.820ml @10:36:15.33 delay 3 seconds @10:36:18.34 ATV.dial PCR @10:36:18.69 AS.reconfigure ASconfig @10:36:18.73 AS.seek empty @10:37:06.83 PCR.program :bleachProfile @10:37:06.90 PCR program should complete in < 2:45 @10:37:06.91 PCR.run 10:00 @10:40:05.69 PCR.off @10:40:05.72 AFV.select :carrierSolution @10:40:05.82 delay 2 seconds @10:40:07.83 AS.seek 1.000ml @10:41:06.50 delay 1 second @10:41:07.51 AFV.select :holdingCoil @10:41:07.62 delay 1 second @10:41:08.63 AS.reconfigure ASfast @10:41:08.68 AS.seek 0.000ml @10:41:36.40 delay 3 seconds @10:41:39.41 AFV.select :carrierSolution @10:41:39.51 delay 2 seconds @10:41:41.52 AS.reconfigure ASconfig @10:41:41.57 AS.seek 1.000ml @10:42:40.24 delay 1 second @10:42:41.25 AFV.select :holdingCoil @10:42:41.36 delay 1 second @10:42:42.37 AS.reconfigure ASfast @10:42:42.42 AS.seek 0.000ml @10:43:10.20 delay 3 seconds @10:43:13.21 AFV.select :carrierSolution @10:43:13.31 delay 2 seconds @10:43:15.32 AS.reconfigure ASconfig @10:43:15.37 AS.seek 1.000ml @10:44:13.97 delay 1 second @10:44:14.98 AFV.select :holdingCoil @10:44:15.08 delay 1 second @10:44:16.09 AS.reconfigure ASfast @10:44:16.14 AS.seek 0.000ml @10:44:43.90 delay 3 seconds @10:44:46.91 AFV.select :carrierSolution @10:44:47.01 delay 2 seconds @10:44:49.02 AS.reconfigure ASconfig @10:44:49.07 AS.seek 1.000ml @10:45:47.70 delay 1 second @10:45:48.71 AFV.select :holdingCoil @10:45:48.82 delay 1 second @10:45:49.83 AS.reconfigure ASfast @10:45:49.88 AS.seek 0.000ml @10:46:17.62 delay 3 seconds @10:46:20.63 AFV.select :carrierSolution @10:46:20.74 delay 2 seconds @10:46:22.75 AS.reconfigure ASconfig @10:46:22.80 AS.seek 1.000ml @10:47:21.42 delay 1 second @10:47:22.43 AFV.select :holdingCoil @10:47:22.53 delay 1 second @10:47:23.54 AS.reconfigure ASnorm @10:47:23.59 AS.seek 0.000ml @10:48:15.04 Setting up PCR for ENTem -- writing /var/log/esp/ENTem14jan2410h.pcr @10:48:15.05 Building PCR Slug from elutionWater with MM2 mix, PP3 primer @10:48:15.06 Priming with carrier @10:48:15.08 ATV.dial ARV @10:48:15.59 ARV.dial :carrier @10:48:15.90 AS.seek 0.500ml @10:48:41.79 AFV.select :carrierSolution @10:48:41.90 delay 2 seconds @10:48:43.91 AS.reconfigure ASconfig @10:48:43.96 AS.seek 1.000ml @10:49:13.40 delay 1 second @10:49:14.41 AFV.select :holdingCoil @10:49:14.51 delay 1 second @10:49:15.52 ATV.dial :waste @10:49:16.78 AS.reconfigure ASnorm @10:49:16.83 AS.seek empty @10:50:08.32 AFV.select :carrierSolution @10:50:08.43 delay 2 seconds @10:50:10.44 AS.reconfigure ASconfig @10:50:10.48 AS.seek 0.500ml @10:50:39.97 delay 1 second @10:50:40.98 AFV.select :holdingCoil @10:50:41.08 Building Slug @10:50:41.09 ATV.dial :ARV @10:50:42.35 delay 2 seconds @10:50:44.36 ARV.dial :air1 @10:50:44.71 delay 2 seconds @10:50:46.77 AS.reconfigure ASslow2 @10:50:46.82 AS.seek 0.506ml @10:50:53.10 delay 2 seconds @10:50:55.11 ARV.dial :MM2 @10:50:56.35 delay 2 seconds @10:50:58.36 Adding MM2 @10:50:58.43 AS.seek 0.524ml @10:51:16.95 delay 25 seconds @10:51:41.96 ARV.dial :PP3 @10:51:42.38 Adding PP3 @10:51:42.40 delay 2 seconds @10:51:44.45 AS.seek 0.530ml @10:51:50.80 delay 10 seconds @10:52:00.81 ARV.dial :air2 @10:52:01.25 adding ARV air @10:52:01.26 delay 2 seconds @10:52:03.32 AS.seek 0.536ml @10:52:09.95 delay 5 seconds @10:52:14.96 ARV.dial :carrier @10:52:16.09 delay 7 seconds @10:52:23.15 AS.seek 0.594ml @10:53:22.56 delay 5 seconds @10:53:27.57 ATV.dial :elutionWater @10:53:28.73 delay 2 seconds @10:53:30.79 AS.seek 0.600ml @10:53:37.09 delay 4 seconds @10:53:41.10 ATV.dial ARV @10:53:42.26 delay 2 seconds @10:53:44.27 ARV.dial :carrier @10:53:44.28 delay 2 seconds @10:53:46.35 AS.seek 0.650ml @10:54:37.68 delay 4 seconds @10:54:41.69 ATV.dial PCR @10:54:42.20 ASV.dial :waste1 @10:54:42.21 ARV.dial :air1 @10:54:42.57 Positioning Slug by 0.324ml @10:54:42.64 AS.seek 0.326ml @11:00:13.17 delay 5 seconds @11:00:18.18 ATV.dial ARV @11:00:18.81 ASV.dial :PCRlock @11:00:19.42 delay 5 seconds @11:00:24.43 Running ENTem ProfileEM60C thermocycling @11:00:24.44 PCR.program ProfileEM60C @11:00:24.57 ProfileEM60C should complete in < 2:11:38 @11:00:24.58 PCR.run 2:49:32.5 @12:57:25.59 PCR.off @12:57:26.13 PCR for ENTem completed -- closed /var/log/esp/ENTem14jan2410h.pcr @12:57:26.19 Cleaning PCR @12:57:26.20 AFV.select :carrierSolution @12:57:26.30 delay 2 seconds @12:57:28.31 AS.reconfigure ASconfig @12:57:28.36 AS.seek 0.500ml @12:57:38.78 delay 1 second @12:57:39.79 AFV.select :holdingCoil @12:57:39.89 ASV.dial :waste1 @12:57:40.49 ATV.dial :air3 @12:57:41.44 AS.reconfigure ASslow1 @12:57:41.49 AS.seek 0.510ml @12:57:43.80 delay 2 seconds @12:57:45.81 ATV.dial :bleach @12:57:46.30 AS.seek 0.810ml @12:58:47.75 delay 3 seconds @12:58:50.76 ATV.dial :air3 @12:58:51.09 delay 3 seconds @12:58:54.15 AS.seek 0.835ml @12:58:59.51 delay 3 seconds @12:59:02.52 ATV.dial :bleach @12:59:02.92 AS.seek 0.820ml @12:59:06.25 delay 3 seconds @12:59:09.26 ARV.dial :waste @12:59:09.76 delay 3 seconds @12:59:12.77 ATV.dial :ARV @12:59:13.61 AS.reconfigure ASconfig @12:59:13.66 AS.seek 0.470ml @12:59:34.47 AFV.select :carrierSolution @12:59:34.58 delay 2 seconds @12:59:36.59 AS.seek 1.000ml @13:00:07.84 delay 1 second @13:00:08.85 AFV.select :holdingCoil @13:00:08.95 delay 1 second @13:00:09.96 AS.reconfigure ASfast @13:00:10.01 AS.seek 0.000ml @13:00:37.76 AFV.select :carrierSolution @13:00:37.87 delay 2 seconds @13:00:39.88 AS.reconfigure ASconfig @13:00:39.93 AS.seek 1.000ml @13:01:38.54 delay 1 second @13:01:39.55 AFV.select :holdingCoil @13:01:39.65 delay 1 second @13:01:40.66 AS.reconfigure ASfast @13:01:40.71 AS.seek 0.000ml @13:02:08.42 AFV.select :carrierSolution @13:02:08.53 delay 2 seconds @13:02:10.54 AS.reconfigure ASconfig @13:02:10.59 AS.seek 1.000ml @13:03:09.17 delay 1 second @13:03:10.18 AFV.select :holdingCoil @13:03:10.28 delay 1 second @13:03:11.29 AS.reconfigure ASfast @13:03:11.34 AS.seek 0.000ml @13:03:39.65 ARV.dial between carrier and air1 @13:03:40.08 ATV.dial :air3 @13:03:41.03 AS.reconfigure ASslow1 @13:03:41.07 AS.seek 0.010ml @13:03:43.44 delay 2 seconds @13:03:45.45 ATV.dial :bleach @13:03:45.94 AS.seek 0.810ml @13:06:29.27 delay 3 seconds @13:06:32.28 ATV.dial :air3 @13:06:32.70 delay 3 seconds @13:06:35.76 AS.seek 0.835ml @13:06:41.13 delay 3 seconds @13:06:44.14 ATV.dial :bleach @13:06:44.53 AS.seek 0.820ml @13:06:47.82 delay 3 seconds @13:06:50.83 ATV.dial PCR @13:06:51.19 AS.reconfigure ASconfig @13:06:51.24 AS.seek empty @13:07:39.35 PCR.program :bleachProfile @13:07:39.41 PCR program should complete in < 2:45 @13:07:39.42 PCR.run 10:00 @13:10:37.62 PCR.off @13:10:37.65 AFV.select :carrierSolution @13:10:37.75 delay 2 seconds @13:10:39.76 AS.seek 1.000ml @13:11:38.44 delay 1 second @13:11:39.45 AFV.select :holdingCoil @13:11:39.56 delay 1 second @13:11:40.57 AS.reconfigure ASfast @13:11:40.62 AS.seek 0.000ml @13:12:08.39 delay 3 seconds @13:12:11.40 AFV.select :carrierSolution @13:12:11.50 delay 2 seconds @13:12:13.51 AS.reconfigure ASconfig @13:12:13.56 AS.seek 1.000ml @13:13:12.18 delay 1 second @13:13:13.19 AFV.select :holdingCoil @13:13:13.29 delay 1 second @13:13:14.30 AS.reconfigure ASfast @13:13:14.35 AS.seek 0.000ml @13:13:42.07 delay 3 seconds @13:13:45.08 AFV.select :carrierSolution @13:13:45.18 delay 2 seconds @13:13:47.19 AS.reconfigure ASconfig @13:13:47.24 AS.seek 1.000ml @13:14:45.85 delay 1 second @13:14:46.86 AFV.select :holdingCoil @13:14:46.97 delay 1 second @13:14:47.98 AS.reconfigure ASfast @13:14:48.03 AS.seek 0.000ml @13:15:15.76 delay 3 seconds @13:15:18.77 AFV.select :carrierSolution @13:15:18.88 delay 2 seconds @13:15:20.89 AS.reconfigure ASconfig @13:15:20.93 AS.seek 1.000ml @13:16:19.52 delay 1 second @13:16:20.53 AFV.select :holdingCoil @13:16:20.63 delay 1 second @13:16:21.64 AS.reconfigure ASfast @13:16:21.69 AS.seek 0.000ml @13:16:49.41 delay 3 seconds @13:16:52.42 AFV.select :carrierSolution @13:16:52.52 delay 2 seconds @13:16:54.53 AS.reconfigure ASconfig @13:16:54.58 AS.seek 1.000ml @13:17:53.13 delay 1 second @13:17:54.14 AFV.select :holdingCoil @13:17:54.24 delay 1 second @13:17:55.25 AS.reconfigure ASnorm @13:17:55.30 AS.seek 0.000ml @13:18:46.78 Setting up PCR for IPCap -- writing /var/log/esp/IPCap14jan2413h.pcr @13:18:46.79 Building PCR Slug from elutionWater with MM1 mix, PP2 primer @13:18:46.80 Priming with carrier @13:18:46.82 ATV.dial ARV @13:18:47.34 ARV.dial :carrier @13:18:47.64 AS.seek 0.500ml @13:19:13.52 AFV.select :carrierSolution @13:19:13.63 delay 2 seconds @13:19:15.64 AS.reconfigure ASconfig @13:19:15.69 AS.seek 1.000ml @13:19:45.15 delay 1 second @13:19:46.16 AFV.select :holdingCoil @13:19:46.26 delay 1 second @13:19:47.27 ATV.dial :waste @13:19:48.63 AS.reconfigure ASnorm @13:19:48.68 AS.seek empty @13:20:40.16 AFV.select :carrierSolution @13:20:40.27 delay 2 seconds @13:20:42.28 AS.reconfigure ASconfig @13:20:42.33 AS.seek 0.500ml @13:21:11.83 delay 1 second @13:21:12.84 AFV.select :holdingCoil @13:21:12.95 Building Slug @13:21:12.96 ATV.dial :ARV @13:21:14.29 delay 2 seconds @13:21:16.30 ARV.dial :air1 @13:21:16.63 delay 2 seconds @13:21:18.69 AS.reconfigure ASslow2 @13:21:18.74 AS.seek 0.506ml @13:21:25.17 delay 2 seconds @13:21:27.18 ARV.dial :MM1 @13:21:27.78 delay 2 seconds @13:21:29.79 Adding MM1 @13:21:29.86 AS.seek 0.524ml @13:21:48.47 delay 25 seconds @13:22:13.48 ARV.dial :PP2 @13:22:13.91 Adding PP2 @13:22:13.93 delay 2 seconds @13:22:15.99 AS.seek 0.530ml @13:22:22.33 delay 10 seconds @13:22:32.34 ARV.dial :air2 @13:22:32.75 adding ARV air @13:22:32.76 delay 2 seconds @13:22:34.82 AS.seek 0.536ml @13:22:41.44 delay 5 seconds @13:22:46.45 ARV.dial :carrier @13:22:47.48 delay 7 seconds @13:22:54.54 AS.seek 0.594ml @13:23:54.22 delay 5 seconds @13:23:59.23 ATV.dial :elutionWater @13:24:00.37 delay 2 seconds @13:24:02.96 AS.seek 0.600ml @13:24:09.58 delay 4 seconds @13:24:13.59 ATV.dial ARV @13:24:14.65 delay 2 seconds @13:24:16.66 ARV.dial :carrier @13:24:16.67 delay 2 seconds @13:24:18.74 AS.seek 0.650ml @13:25:10.10 delay 4 seconds @13:25:14.11 ATV.dial PCR @13:25:14.73 ASV.dial :waste1 @13:25:14.74 ARV.dial :air1 @13:25:15.10 Positioning Slug by 0.324ml @13:25:15.17 AS.seek 0.326ml @13:30:45.84 delay 5 seconds @13:30:50.85 ATV.dial ARV @13:30:51.36 ASV.dial :PCRlock @13:30:51.70 delay 5 seconds @13:30:52.40 Session Ended @13:30:56.71 Running IPCap Profile59C thermocycling @13:30:56.72 PCR.program Profile59C @13:30:56.82 Profile59C should complete in < 1:39:08 @13:30:56.83 PCR.run 2:08:55 @14:55:33.01 PCR.off @14:55:33.54 PCR for IPCap completed -- closed /var/log/esp/IPCap14jan2413h.pcr @14:55:33.59 Cleaning PCR @14:55:33.60 AFV.select :carrierSolution @14:55:33.69 delay 2 seconds @14:55:35.70 AS.reconfigure ASconfig @14:55:35.73 AS.seek 0.500ml @14:55:46.19 delay 1 second @14:55:47.20 AFV.select :holdingCoil @14:55:47.29 ASV.dial :waste1 @14:55:47.77 ATV.dial :air3 @14:55:48.78 AS.reconfigure ASslow1 @14:55:48.82 AS.seek 0.510ml @14:55:51.10 delay 2 seconds @14:55:53.11 ATV.dial :bleach @14:55:53.49 AS.seek 0.810ml @14:56:54.92 delay 3 seconds @14:56:57.93 ATV.dial :air3 @14:56:58.25 delay 3 seconds @14:57:01.31 AS.seek 0.835ml @14:57:06.66 delay 3 seconds @14:57:09.67 ATV.dial :bleach @14:57:10.05 AS.seek 0.820ml @14:57:13.34 delay 3 seconds @14:57:16.35 ARV.dial :waste @14:57:16.94 delay 3 seconds @14:57:19.95 ATV.dial :ARV @14:57:20.68 AS.reconfigure ASconfig @14:57:20.72 AS.seek 0.470ml @14:57:41.40 AFV.select :carrierSolution @14:57:41.50 delay 2 seconds @14:57:43.51 AS.seek 1.000ml @14:58:14.75 delay 1 second @14:58:15.76 AFV.select :holdingCoil @14:58:15.85 delay 1 second @14:58:16.86 AS.reconfigure ASfast @14:58:16.89 AS.seek 0.000ml @14:58:44.56 AFV.select :carrierSolution @14:58:44.66 delay 2 seconds @14:58:46.67 AS.reconfigure ASconfig @14:58:46.70 AS.seek 1.000ml @14:59:45.29 delay 1 second @14:59:46.30 AFV.select :holdingCoil @14:59:46.39 delay 1 second @14:59:47.40 AS.reconfigure ASfast @14:59:47.43 AS.seek 0.000ml @15:00:15.13 AFV.select :carrierSolution @15:00:15.23 delay 2 seconds @15:00:17.24 AS.reconfigure ASconfig @15:00:17.27 AS.seek 1.000ml @15:01:15.89 delay 1 second @15:01:16.90 AFV.select :holdingCoil @15:01:16.99 delay 1 second @15:01:18.00 AS.reconfigure ASfast @15:01:18.03 AS.seek 0.000ml @15:01:45.76 ARV.dial between carrier and air1 @15:01:46.17 ATV.dial :air3 @15:01:47.10 AS.reconfigure ASslow1 @15:01:47.13 AS.seek 0.010ml @15:01:49.47 delay 2 seconds @15:01:51.48 ATV.dial :bleach @15:01:51.86 AS.seek 0.810ml @15:04:35.26 delay 3 seconds @15:04:38.27 ATV.dial :air3 @15:04:38.60 delay 3 seconds @15:04:41.66 AS.seek 0.835ml @15:04:47.01 delay 3 seconds @15:04:50.02 ATV.dial :bleach @15:04:50.40 AS.seek 0.820ml @15:04:53.70 delay 3 seconds @15:04:56.71 ATV.dial PCR @15:04:57.05 AS.reconfigure ASconfig @15:04:57.08 AS.seek empty @15:05:45.21 PCR.program :bleachProfile @15:05:45.26 PCR program should complete in < 2:45 @15:05:45.27 PCR.run 10:00 @15:08:43.52 PCR.off @15:08:43.55 AFV.select :carrierSolution @15:08:43.65 delay 2 seconds @15:08:45.66 AS.seek 1.000ml @15:09:44.31 delay 1 second @15:09:45.32 AFV.select :holdingCoil @15:09:45.41 delay 1 second @15:09:46.42 AS.reconfigure ASfast @15:09:46.46 AS.seek 0.000ml @15:10:14.17 delay 3 seconds @15:10:17.18 AFV.select :carrierSolution @15:10:17.27 delay 2 seconds @15:10:19.28 AS.reconfigure ASconfig @15:10:19.31 AS.seek 1.000ml @15:11:17.90 delay 1 second @15:11:18.91 AFV.select :holdingCoil @15:11:19.00 delay 1 second @15:11:20.01 AS.reconfigure ASfast @15:11:20.04 AS.seek 0.000ml @15:11:47.76 delay 3 seconds @15:11:50.77 AFV.select :carrierSolution @15:11:50.86 delay 2 seconds @15:11:52.87 AS.reconfigure ASconfig @15:11:52.90 AS.seek 1.000ml @15:12:51.49 delay 1 second @15:12:52.50 AFV.select :holdingCoil @15:12:52.59 delay 1 second @15:12:53.60 AS.reconfigure ASfast @15:12:53.63 AS.seek 0.000ml @15:13:21.37 delay 3 seconds @15:13:24.38 AFV.select :carrierSolution @15:13:24.47 delay 2 seconds @15:13:26.48 AS.reconfigure ASconfig @15:13:26.51 AS.seek 1.000ml @15:14:25.10 delay 1 second @15:14:26.11 AFV.select :holdingCoil @15:14:26.30 delay 1 second @15:14:27.31 AS.reconfigure ASfast @15:14:27.34 AS.seek 0.000ml @15:14:55.08 delay 3 seconds @15:14:58.11 AFV.select :carrierSolution @15:14:58.20 delay 2 seconds @15:15:00.21 AS.reconfigure ASconfig @15:15:00.24 AS.seek 1.000ml @15:15:58.85 delay 1 second @15:15:59.86 AFV.select :holdingCoil @15:15:59.95 delay 1 second @15:16:00.96 AS.reconfigure ASnorm @15:16:00.99 AS.seek 0.000ml @15:16:52.44 Setting up PCR for IPCem -- writing /var/log/esp/IPCem14jan2415h.pcr @15:16:52.45 Building PCR Slug from elutionWater with MM3 mix, PP5 primer @15:16:52.46 Priming with carrier ATV.dial ARV @15:16:52.97 ARV.dial :carrier @15:16:53.25 AS.seek 0.500ml @15:17:19.12 AFV.select :carrierSolution @15:17:19.21 delay 2 seconds @15:17:21.22 AS.reconfigure ASconfig @15:17:21.25 AS.seek 1.000ml @15:17:50.70 delay 1 second @15:17:51.71 AFV.select :holdingCoil @15:17:51.80 delay 1 second @15:17:52.81 ATV.dial :waste @15:17:54.05 AS.reconfigure ASnorm @15:17:54.08 AS.seek empty @15:18:45.55 AFV.select :carrierSolution @15:18:45.65 delay 2 seconds @15:18:47.66 AS.reconfigure ASconfig @15:18:47.69 AS.seek 0.500ml @15:19:17.16 delay 1 second @15:19:18.17 AFV.select :holdingCoil @15:19:18.26 Building Slug @15:19:18.27 ATV.dial :ARV @15:19:19.61 delay 2 seconds @15:19:21.62 ARV.dial :air1 @15:19:21.94 delay 2 seconds @15:19:24.00 AS.reconfigure ASslow2 @15:19:24.03 AS.seek 0.506ml @15:19:30.97 delay 2 seconds @15:19:32.98 ARV.dial :MM3 @15:19:33.83 delay 2 seconds @15:19:35.84 Adding MM3 @15:19:35.90 AS.seek 0.524ml @15:19:54.55 delay 25 seconds @15:20:19.56 ARV.dial :PP5 @15:20:19.88 Adding PP5 @15:20:19.89 delay 2 seconds @15:20:21.95 AS.seek 0.530ml @15:20:28.42 delay 10 seconds @15:20:38.43 ARV.dial :air3 @15:20:38.88 adding ARV air @15:20:38.89 delay 2 seconds @15:20:40.95 AS.seek 0.536ml @15:20:47.21 delay 5 seconds @15:20:52.22 ARV.dial :carrier @15:20:53.24 delay 7 seconds @15:21:00.30 AS.seek 0.594ml @15:21:59.72 delay 5 seconds @15:22:04.73 ATV.dial :elutionWater @15:22:05.78 delay 2 seconds @15:22:07.84 AS.seek 0.600ml @15:22:14.44 delay 4 seconds @15:22:18.45 ATV.dial ARV @15:22:19.61 delay 2 seconds @15:22:21.62 ARV.dial :carrier @15:22:21.63 delay 2 seconds @15:22:23.68 AS.seek 0.650ml @15:23:14.97 delay 4 seconds @15:23:18.98 ATV.dial PCR @15:23:19.48 ASV.dial :waste1 @15:23:19.49 ARV.dial :air1 @15:23:19.84 Positioning Slug by 0.324ml @15:23:19.89 AS.seek 0.326ml @15:28:50.53 delay 5 seconds @15:28:55.54 ATV.dial ARV @15:28:56.05 ASV.dial :PCRlock @15:28:56.53 delay 5 seconds @15:29:01.54 Running IPCem ProfileEM59C thermocycling @15:29:01.55 PCR.program ProfileEM59C @15:29:01.66 ProfileEM59C should complete in < 2:11:38 @15:29:01.67 PCR.run 2:49:32.5 @17:26:43.66 PCR.off @17:26:44.73 PCR for IPCem completed -- closed /var/log/esp/IPCem14jan2415h.pcr @17:26:44.78 Cleaning PCR @17:26:44.79 AFV.select :carrierSolution @17:26:44.88 delay 2 seconds @17:26:46.89 AS.reconfigure ASconfig @17:26:46.93 AS.seek 0.500ml @17:26:57.36 delay 1 second @17:26:58.37 AFV.select :holdingCoil @17:26:58.46 ASV.dial :waste1 @17:26:59.21 ATV.dial :air3 @17:27:00.16 AS.reconfigure ASslow1 @17:27:00.19 AS.seek 0.510ml @17:27:02.47 delay 2 seconds @17:27:04.48 ATV.dial :bleach @17:27:04.96 AS.seek 0.810ml @17:28:06.41 delay 3 seconds @17:28:09.42 ATV.dial :air3 @17:28:09.75 delay 3 seconds @17:28:12.81 AS.seek 0.835ml @17:28:18.14 delay 3 seconds @17:28:21.15 ATV.dial :bleach @17:28:21.55 AS.seek 0.820ml @17:28:24.86 delay 3 seconds @17:28:27.87 ARV.dial :waste @17:28:28.37 delay 3 seconds @17:28:31.38 ATV.dial :ARV @17:28:32.10 AS.reconfigure ASconfig @17:28:32.14 AS.seek 0.470ml @17:28:52.83 AFV.select :carrierSolution @17:28:52.92 delay 2 seconds @17:28:54.93 AS.seek 1.000ml @17:29:26.15 delay 1 second @17:29:27.16 AFV.select :holdingCoil @17:29:27.25 delay 1 second @17:29:28.26 AS.reconfigure ASfast @17:29:28.29 AS.seek 0.000ml @17:29:56.00 AFV.select :carrierSolution @17:29:56.10 delay 2 seconds @17:29:58.11 AS.reconfigure ASconfig @17:29:58.14 AS.seek 1.000ml @17:30:56.71 delay 1 second @17:30:57.72 AFV.select :holdingCoil @17:30:57.81 delay 1 second @17:30:58.82 AS.reconfigure ASfast @17:30:58.85 AS.seek 0.000ml @17:31:26.56 AFV.select :carrierSolution @17:31:26.66 delay 2 seconds @17:31:28.67 AS.reconfigure ASconfig @17:31:28.70 AS.seek 1.000ml @17:32:27.27 delay 1 second @17:32:28.28 AFV.select :holdingCoil @17:32:28.37 delay 1 second @17:32:29.38 AS.reconfigure ASfast @17:32:29.41 AS.seek 0.000ml @17:32:57.13 ARV.dial between carrier and air1 @17:32:57.54 ATV.dial :air3 @17:32:58.57 AS.reconfigure ASslow1 @17:32:58.60 AS.seek 0.010ml @17:33:00.94 delay 2 seconds @17:33:02.95 ATV.dial :bleach @17:33:03.32 AS.seek 0.810ml @17:35:46.79 delay 3 seconds @17:35:49.80 ATV.dial :air3 @17:35:50.12 delay 3 seconds @17:35:53.18 AS.seek 0.835ml @17:35:58.54 delay 3 seconds @17:36:01.55 ATV.dial :bleach @17:36:01.92 AS.seek 0.820ml @17:36:05.21 delay 3 seconds @17:36:08.22 ATV.dial PCR @17:36:08.55 AS.reconfigure ASconfig @17:36:08.58 AS.seek empty @17:36:56.71 PCR.program :bleachProfile @17:36:56.77 PCR program should complete in < 2:45 @17:36:56.78 PCR.run 10:00 @17:39:54.97 PCR.off @17:39:55.00 AFV.select :carrierSolution @17:39:55.09 delay 2 seconds @17:39:57.10 AS.seek 1.000ml @17:40:55.73 delay 1 second @17:40:56.74 AFV.select :holdingCoil @17:40:56.83 delay 1 second @17:40:57.84 AS.reconfigure ASfast @17:40:57.87 AS.seek 0.000ml @17:41:25.59 delay 3 seconds @17:41:28.60 AFV.select :carrierSolution @17:41:28.69 delay 2 seconds @17:41:30.70 AS.reconfigure ASconfig @17:41:30.73 AS.seek 1.000ml @17:42:29.32 delay 1 second @17:42:30.33 AFV.select :holdingCoil @17:42:30.42 delay 1 second @17:42:31.43 AS.reconfigure ASfast @17:42:31.46 AS.seek 0.000ml @17:42:59.20 delay 3 seconds @17:43:02.21 AFV.select :carrierSolution @17:43:02.30 delay 2 seconds @17:43:04.31 AS.reconfigure ASconfig @17:43:04.34 AS.seek 1.000ml @17:44:02.93 delay 1 second @17:44:03.94 AFV.select :holdingCoil @17:44:04.03 delay 1 second @17:44:05.04 AS.reconfigure ASfast @17:44:05.07 AS.seek 0.000ml @17:44:32.79 delay 3 seconds @17:44:35.80 AFV.select :carrierSolution @17:44:35.89 delay 2 seconds @17:44:37.90 AS.reconfigure ASconfig @17:44:37.93 AS.seek 1.000ml @17:45:36.55 delay 1 second @17:45:37.56 AFV.select :holdingCoil @17:45:37.65 delay 1 second @17:45:38.66 AS.reconfigure ASfast @17:45:38.69 AS.seek 0.000ml @17:46:06.41 delay 3 seconds @17:46:09.42 AFV.select :carrierSolution @17:46:09.51 delay 2 seconds @17:46:11.52 AS.reconfigure ASconfig @17:46:11.55 AS.seek 1.000ml @17:47:10.17 delay 1 second @17:47:11.18 AFV.select :holdingCoil @17:47:11.39 delay 1 second @17:47:12.40 AS.reconfigure ASnorm @17:47:12.43 AS.seek 0.000ml @17:48:03.86 AS.stop @17:48:03.88 CC.stop @17:48:03.91 CS.stop @17:48:03.93 PC.stop @17:48:03.96 PS.stop @17:48:03.98 Forearm.stop @17:48:04.46 Elbow.stop @17:48:04.49 SS.stop @17:48:04.55 Sleepy.power :raw,:OFF @17:48:04.57 Sleepy.power :dwarf,:OFF Shutdown @17:48:04.58 GoodNews.email "MFBPCR completed",Subject => "Shutdown" @17:48:06.78
delayUntil "13:00:00.00PST22-Feb-14" @13:19:57.84PST31-Jan-14 Session Begun @13:20:05.32 -> ps @13:20:05.33 NameError in esp -- undefined local variable or method `ps' for # @13:21:11.41 Session Begun @13:21:19.97 -> ps @13:21:19.98 NameError in kevan -- undefined local variable or method `ps' for # @13:22:00.18 -> MainThread.abort @13:22:00.20 Thread::Aborted in MAIN -- Aborted by kevan @13:22:00.23
Waiting to Resume from Checkpoint "Garaging FlushPuck in Collection Clamp" @13:22:00.25 BadNews.email "Aborted by kevan",Subject => "Thread::Aborted in MAIN" @13:22:38.54 -> exit @13:22:38.56 Session Ended @13:22:59.06 -> exit @13:22:59.07 Session Ended @13:25:58.49 Session Begun @13:26:45.28 -> abort! "end of 3 peat" CmdServer::Client::Error in kevan -- Aborting Mission: end of 3 peat @13:26:45.31 BadNews.email "end of 3 peat",Subject => "14jan21SCCWRP ABORTED!" @13:26:58.91 Session Ended @17:33:29.34PST11-Feb-14 ESP2 v3.20 I2C Gateway v3.01 @17:33:32.43
Configuration for neo by maro@mbari.org /home/esp/esp2/shallow/neo/configure.rb @17:33:32.52 Configuration for MFB10 by preston@mbari.org /home/esp/esp2/mfb/MFB10/configure.rb @17:33:35.94 Sleepy.power :raw,:ON @17:33:36.64 SPE.coast @17:33:36.68 SPE.configure SPEconfig @17:33:36.71 AS.stop @17:33:36.74 AS.configure ASconfig @17:33:36.77 CC.stop @17:33:36.79 CC.configure CCconfig @17:33:36.82 CS.stop @17:33:36.84 CS.configure CSconfig @17:33:36.87 PC.stop @17:33:36.89 PC.configure PCconfig @17:33:36.92 PS.stop @17:33:36.94 PS.configure PSconfig @17:33:37.00 Forearm.stop @17:33:37.02 Forearm.configure LooseForearm @17:33:37.05 Elbow.stop @17:33:37.07 Elbow.configure LooseElbow @17:33:37.13 SC.stop @17:33:37.15 SC.configure SCloose @17:33:37.18 SE.stop @17:33:37.21 SE.configure SEconfig @17:33:37.24 SS.stop @17:33:37.26 SS.configure SSconfig @17:33:37.29 CH.coast @17:33:37.34 CH.configure CHconfig @17:33:37.36 PH.coast @17:33:37.40 PH.configure PHconfig @17:33:37.44 PCR.off @17:33:37.46 PCR.pidtemp= 50,0.1,0,300,25,25 @17:33:37.49 PCR.caltemp= 6.6661,0.0344,0.0 @17:33:37.52 All dwarves are running firmware version 3.74 @17:33:38.08 CTD.latitude = 36.8044 Degrees North in seawater @17:33:38.09 Initializing Sensors @17:33:38.12 GoodNews.email "Starting w/pucks in tube 2\n Ending 09:00PST12-Feb-14",Subject => "14feb11SCCWRP begun" @17:33:39.70
Asleep til 5:40PM 2/11/14 @17:33:39.75 Sleeping until 17:40 @17:33:39.77 Sleepy.power :raw,:OFF @17:33:39.78 Sleepy.power :dwarf,:OFF Shutdown @17:33:51.18 GoodNews.email "Then, in tube 2: wqPCR\n/home/esp/esp2/mission/14feb11SCCWRP.rb:48",Subject => "Asleep until 17:40" @17:34:03.11 Instrument::ReadTimeout in pollContext -- Could not get prompt from CTD on ctd @17:34:03.27 BadNews.email "Could not get prompt from CTD on ctd",Subject => "Instrument::ReadTimeout in pollContext" @17:40:00.34
Powering up Dwarves @17:40:05.27 Sleepy.power :raw,:ON @17:40:05.72 SPE.configure SPEconfig @17:40:05.74 AS.configure ASconfig @17:40:05.78 CC.configure CCconfig @17:40:05.81 CS.configure CSconfig @17:40:05.84 PC.configure PCconfig @17:40:05.87 PS.configure PSconfig @17:40:05.90 Forearm.configure LooseForearm @17:40:05.93 Elbow.configure LooseElbow @17:40:05.96 SC.configure SCloose @17:40:05.99 SE.configure SEconfig @17:40:06.02 SS.configure SSconfig @17:40:06.06 CH.configure CHconfig @17:40:06.08 PH.configure PHconfig @17:40:06.11 Valve.reset @17:40:06.77 PCR.off @17:40:06.79 PCR.pidtemp= 50,0.1,0,300,25,25 @17:40:06.82 PCR.caltemp= 6.6661,0.0344,0.0 @17:40:06.92 Intake.select :close @17:40:06.95 Exhaust.select :close CTV.dial :air @17:40:06.99 PSV.dial :air @17:40:07.03
PC.reconfigure PCconfig @17:40:07.09 PC.home.seek open @17:40:07.12 CSV.dial :waste1 @17:40:07.18 PS.reconfigure PSconfig @17:40:07.22 PS.home.seek 0.000ml @17:40:07.64 SSV.dial :spit,avoiding => exhaust @17:40:07.76 CS.reconfigure CSfast @17:40:07.79 SS.reconfigure SSconfig @17:40:07.88 SS.home.seek 0.00ml @17:40:07.90 CS.home.seek 0.000ml @17:40:07.98 CC.reconfigure CCconfig @17:40:08.01 CC.home.seek open @17:40:17.42 PS.coast @17:40:17.51
CC.seek open @17:40:17.52 PC.seek open @17:40:17.68
Forearm.reconfigure LooseForearm @17:40:17.71 Forearm.home.seek retract @17:40:18.46 Hand.grip @17:40:18.48
Elbow.jog -1500 @17:40:19.00 Elbow.reconfigure LooseElbow @17:40:19.03 Elbow.home.seek clear @17:40:24.01 SE.reconfigure SEconfig @17:40:24.04 SE.home.seek down @17:40:24.61 SC.reconfigure SCsloppy @17:40:24.65 SC.home.seek initial @17:40:28.40 Forearm.seek retract @17:40:28.46 Elbow.seek hammer @17:40:29.65 Forearm.reconfigure TightForearm @17:40:29.70 Forearm.seek 2 @17:40:30.84 Forearm.seek retract @17:40:31.99 Forearm.reconfigure LooseForearm @17:40:32.29PST11-Feb-14 ===> STARTED wqPCR (phase #1) <=== @17:40:32.38 Starting mfb @17:40:32.39 pcrBAC sampling at most 1000ml, wcr at most 1000ml @17:40:32.42 Loading sh1 puck from tube 2 into Collection Clamp @17:40:32.48 BACfans.email "Can@17:40:32, 23.3C, 39% humidity, 14.5psia, 13.674V, 0.707A, 0.701A avg, 9.67W\nCTD data unavailable\nISUS data unavailable",Subject => "pcrBAC sampling at most 1000ml, wcr at most 1000ml" @17:40:33.50 Readying the MFB @17:40:33.53 Homing MFB Syringe AFV.select :holdingCoil @17:40:33.57
Hand.release @17:40:33.66 ATV.dial :air2 @17:40:34.52
SC.reconfigure SCloose @17:40:34.65 Elbow.reconfigure TightElbow @17:40:34.69 Elbow.seek 2 @17:40:34.71 AS.reconfigure ASconfig @17:40:34.80 AS.home.seek home @17:40:34.94
Forearm.reconfigure TightForearm @17:40:34.97 Forearm.seek 2 + 800 counts @17:40:35.97 SE.reconfigure SEpick @17:40:36.03 SE.seek beamBreak @17:40:36.88 SE.configure SEconfig @17:40:37.01 Elbow.seek 2 @17:40:37.18 Forearm.seek 2 + 300 counts @17:40:37.38 Forearm.reconfigure LooseForearm @17:40:37.42 Hand.grip @17:40:38.12 SE.seek down @17:40:38.26
Forearm.seek retract @17:40:38.84 SE.coast @17:40:39.22
Elbow.seek CC @17:40:40.82 Elbow.reconfigure LooseElbow @17:40:40.90 Forearm.reconfigure TightForearm @17:40:40.93 Forearm.seek CC @17:40:41.95 CC.seek closed @17:40:42.35 AFV.select :carrierSolution @17:40:42.44 delay 2 seconds @17:40:44.48 AS.seek 1.000ml @17:40:45.72
Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.99 @17:40:45.73 Hand.release @17:40:46.52 Forearm.reconfigure LooseForearm @17:40:46.55 Forearm.seek retract @17:40:47.49 SC.coast @17:40:47.52 collectSample 1000ml w/bubble point of 55psi @17:40:47.53 Priming 7ml Intake.select :close @17:40:47.54 Exhaust.select :close @17:40:47.55 SS.hold @17:40:47.71 Calibrating pressure and temperature sensors @17:40:47.73 CTV.dial :air @17:40:47.74 SSV.dial :spit,avoiding => exhaust @17:40:47.75 SS.reconfigure SSfast @17:40:47.78 SS.seek 5.00ml @17:40:50.67 SSV.dial CBV @17:40:50.69
CBV.dial SSV @17:40:52.01 delay 3.8 seconds @17:40:55.89 SSV.dial :spit @17:40:57.18 SS.seek 0.00ml @17:40:59.93 SSV.dial CBV @17:41:01.23 CTV.dial :intake @17:41:01.99 SSV.dial CBV @17:41:02.00
CBV.dial SSV @17:41:02.01 Allowing 55s for 7.0ml of remaining 7.0ml @17:41:02.02 Intake.select :open @17:41:04.16 Intake @34.4psia, vacuum from 20.0 to 24.0psi @17:41:04.17 SS.reconfigure forSampling @17:41:04.21 SS.seek 7.00ml @17:41:08.22 Vacuum too high -- waiting up to 40s @17:41:08.48 Intake @27.4psia, vacuum from 20.0 to 24.0psi @17:41:08.49 SS.reconfigure forSampling @17:41:08.52 SS.seek 7.00ml @17:41:10.70 Stroke completed -- waiting for pressure to equalize ... @17:41:11.34 Intake.select :close @17:41:11.47 SSV.dial :exhaust @17:41:12.23 Exhaust.select :open @17:41:12.32 SS.reconfigure SSexhale @17:41:12.36 SS.seek 0.00ml @17:41:15.89 Exhaust.select :close @17:41:15.98 Sampling 1000.0ml @17:41:15.99 Intake.select :close @17:41:16.00 Exhaust.select :close @17:41:16.01 SS.reconfigure SSconfig @17:41:16.04 SS.hold @17:41:16.19 CTV.dial :intake @17:41:16.20 SSV.dial CBV @17:41:16.22
CBV.dial SSV @17:41:16.97 Allowing 185s for 25.0ml of remaining 1000.0ml Intake.select :open @17:41:19.10 Intake @34.2psia, vacuum from 20.0 to 24.0psi @17:41:19.11 SS.reconfigure forSampling @17:41:19.15 SS.seek 25.00ml @17:41:22.18 Vacuum too high -- waiting up to 40s @17:41:22.46 Intake @29.3psia, vacuum from 20.0 to 24.0psi SS.reconfigure forSampling @17:41:22.50 SS.seek 25.00ml @17:41:25.65 Vacuum too high -- waiting up to 40s @17:41:26.14 Intake @30.0psia, vacuum from 20.0 to 24.0psi @17:41:26.15 SS.reconfigure forSampling @17:41:26.18 SS.seek 25.00ml @17:41:30.40 Vacuum too high -- waiting up to 40s @17:41:30.89 Intake @30.8psia, vacuum from 20.0 to 24.0psi @17:41:30.90 SS.reconfigure forSampling @17:41:30.94 SS.seek 25.00ml @17:41:36.18 Vacuum too high -- waiting up to 40s @17:41:36.21 delay 1 second @17:41:36.67
Intake @31.5psia, vacuum from 20.0 to 24.0psi @17:41:36.68 SS.reconfigure forSampling @17:41:36.71 SS.seek 25.00ml @17:41:37.22 AFV.select :holdingCoil @17:41:37.31 delay 1 second @17:41:38.32 ATV.dial :waste @17:41:39.22 AS.reconfigure ASnorm @17:41:39.25 AS.home.seek empty @17:41:43.92
Stroke completed -- waiting for pressure to equalize ... @17:41:44.56 Intake.select :close @17:41:44.69 SSV.dial :exhaust @17:41:45.43 Exhaust.select :open @17:41:45.54 SS.reconfigure SSexhale @17:41:45.58 SS.seek 0.00ml @17:41:54.31 Exhaust.select :close @17:41:54.40 SSV.dial CBV @17:41:54.42
CBV.dial SSV @17:41:55.17 Allowing 185s for 25.0ml of remaining 975.0ml @17:41:55.18 Intake.select :open @17:41:57.31 Intake @34.2psia, vacuum from 20.0 to 24.0psi @17:41:57.32 SS.reconfigure forSampling @17:41:57.36 SS.seek 25.00ml @17:42:00.34 Vacuum too high -- waiting up to 40s @17:42:00.60 Intake @31.5psia, vacuum from 20.0 to 24.0psi @17:42:00.61 SS.reconfigure forSampling @17:42:00.65 SS.seek 25.00ml @17:42:27.99 Stroke completed -- waiting for pressure to equalize ... @17:42:28.63 Intake.select :close @17:42:28.76 SSV.dial :exhaust @17:42:29.49 Exhaust.select :open @17:42:29.59 SS.reconfigure SSexhale @17:42:29.63 SS.seek 0.00ml @17:42:32.52 ATV.dial :waste @17:42:32.53 ARV.dial between carrier and air1 @17:42:32.60 ASV.dial :waste1 @17:42:38.73
Exhaust.select :close @17:42:38.82 SSV.dial CBV @17:42:38.84
CBV.dial SSV @17:42:39.60 Allowing 185s for 25.0ml of remaining 950.0ml Intake.select :open @17:42:41.73 Intake @34.2psia, vacuum from 20.0 to 24.0psi @17:42:41.74 SS.reconfigure forSampling @17:42:41.78 SS.seek 25.00ml @17:42:56.44 Session Begun @17:43:11.99
Stroke completed -- waiting for pressure to equalize ... @17:43:12.63 Intake.select :close @17:43:12.77 SSV.dial :exhaust @17:43:13.54 Exhaust.select :open @17:43:13.65 SS.reconfigure SSexhale @17:43:13.70 SS.seek 0.00ml @17:43:22.36 Exhaust.select :close @17:43:22.46 SSV.dial CBV @17:43:22.48
CBV.dial SSV @17:43:23.27 Allowing 185s for 25.0ml of remaining 925.0ml @17:43:23.29 Intake.select :open @17:43:25.44 Intake @34.2psia, vacuum from 20.0 to 24.0psi @17:43:25.45 SS.reconfigure forSampling @17:43:25.51 SS.seek 25.00ml @17:43:41.65 -> extSamplerReady=Power.on @17:43:53.17
Vacuum too high -- waiting up to 40s @17:43:53.44 Intake @32.0psia, vacuum from 20.0 to 24.0psi @17:43:53.45 SS.reconfigure forSampling @17:43:53.50 SS.seek 25.00ml @17:43:57.07 Stroke completed -- waiting for pressure to equalize ... @17:43:57.77 Intake.select :close @17:43:57.98 SSV.dial :exhaust @17:43:58.75 Exhaust.select :open @17:43:58.86 SS.reconfigure SSexhale @17:43:58.91 SS.seek 0.00ml @17:44:07.53 Exhaust.select :close @17:44:07.63 SSV.dial CBV @17:44:07.65
CBV.dial SSV @17:44:08.44 Allowing 185s for 25.0ml of remaining 900.0ml @17:44:08.46 Intake.select :open @17:44:10.61 Intake @34.2psia, vacuum from 20.0 to 24.0psi @17:44:10.62 SS.reconfigure forSampling @17:44:10.68 SS.seek 25.00ml @17:44:21.72 -> extSamplerReady=Power.off @17:44:50.67
Stroke completed -- waiting for pressure to equalize ... @17:44:51.31 Intake.select :close @17:44:51.45 SSV.dial :exhaust @17:44:52.20 Exhaust.select :open @17:44:52.31 SS.reconfigure SSexhale @17:44:52.37 SS.seek 0.00ml @17:45:01.05 Exhaust.select :close @17:45:01.15 SSV.dial CBV @17:45:01.17
CBV.dial SSV @17:45:01.94 Allowing 185s for 25.0ml of remaining 875.0ml @17:45:01.95 Intake.select :open @17:45:04.12 Intake @34.2psia, vacuum from 20.0 to 24.0psi @17:45:04.13 SS.reconfigure forSampling @17:45:04.18 SS.seek 25.00ml @17:45:17.53 -> S @17:45:17.54 NameError in kevan -- uninitialized constant S @17:45:44.18
Stroke completed -- waiting for pressure to equalize ... @17:45:44.82 Intake.select :close @17:45:44.96 SSV.dial :exhaust @17:45:45.72 Exhaust.select :open @17:45:45.83 SS.reconfigure SSexhale @17:45:45.88 SS.seek 0.00ml @17:45:51.61 -> Sleepy.power.on @17:45:51.70 NoMethodError in kevan -- undefined method `on' for # @17:45:54.56
Exhaust.select :close @17:45:54.66 SSV.dial CBV @17:45:54.68
CBV.dial SSV @17:45:55.45 Allowing 185s for 25.0ml of remaining 850.0ml @17:45:55.46 Intake.select :open @17:45:57.62 Intake @34.3psia, vacuum from 20.0 to 24.0psi @17:45:57.63 SS.reconfigure forSampling @17:45:57.69 SS.seek 25.00ml @17:46:38.29 Stroke completed -- waiting for pressure to equalize ... @17:46:38.93 Intake.select :close @17:46:39.07 SSV.dial :exhaust @17:46:39.82 Exhaust.select :open @17:46:39.94 SS.reconfigure SSexhale @17:46:40.00 SS.seek 0.00ml @17:46:48.49 Exhaust.select :close @17:46:48.59 SSV.dial CBV @17:46:48.61
CBV.dial SSV @17:46:49.38 Allowing 185s for 25.0ml of remaining 825.0ml @17:46:49.39 Intake.select :open @17:46:51.55 Intake @34.5psia, vacuum from 20.0 to 24.0psi @17:46:51.56 SS.reconfigure forSampling @17:46:51.62 SS.seek 25.00ml @17:47:31.65 Stroke completed -- waiting for pressure to equalize ... @17:47:32.29 Intake.select :close @17:47:32.43 SSV.dial :exhaust @17:47:33.18 Exhaust.select :open @17:47:33.30 SS.reconfigure SSexhale @17:47:33.35 SS.seek 0.00ml @17:47:41.94 Exhaust.select :close @17:47:42.06 SSV.dial CBV @17:47:42.08
CBV.dial SSV @17:47:42.85 Allowing 185s for 25.0ml of remaining 800.0ml @17:47:42.86 Intake.select :open @17:47:45.03 Intake @34.5psia, vacuum from 20.0 to 24.0psi @17:47:45.04 SS.reconfigure forSampling @17:47:45.10 SS.seek 25.00ml @17:48:25.10 Stroke completed -- waiting for pressure to equalize ... @17:48:25.74 Intake.select :close @17:48:25.88 SSV.dial :exhaust @17:48:26.63 Exhaust.select :open @17:48:26.74 SS.reconfigure SSexhale @17:48:26.79 SS.seek 0.00ml @17:48:35.21 Exhaust.select :close @17:48:35.32 SSV.dial CBV @17:48:35.34
CBV.dial SSV @17:48:36.12 Allowing 185s for 25.0ml of remaining 775.0ml @17:48:36.13 Intake.select :open @17:48:38.28 Intake @34.5psia, vacuum from 20.0 to 24.0psi @17:48:38.29 SS.reconfigure forSampling @17:48:38.35 SS.seek 25.00ml @17:49:18.28 Stroke completed -- waiting for pressure to equalize ... @17:49:18.92 Intake.select :close @17:49:19.07 SSV.dial :exhaust @17:49:19.83 Exhaust.select :open @17:49:19.94 SS.reconfigure SSexhale @17:49:19.99 SS.seek 0.00ml @17:49:28.59 Exhaust.select :close @17:49:28.70 SSV.dial CBV @17:49:28.72
CBV.dial SSV @17:49:29.50 Allowing 185s for 25.0ml of remaining 750.0ml @17:49:29.51 Intake.select :open @17:49:31.66 Intake @34.5psia, vacuum from 20.0 to 24.0psi @17:49:31.67 SS.reconfigure forSampling @17:49:31.73 SS.seek 25.00ml @17:50:11.69 Stroke completed -- waiting for pressure to equalize ... @17:50:12.91 Intake.select :close @17:50:13.05 SSV.dial :exhaust @17:50:13.83 Exhaust.select :open @17:50:13.94 SS.reconfigure SSexhale @17:50:13.99 SS.seek 0.00ml @17:50:22.58 Exhaust.select :close @17:50:22.69 SSV.dial CBV @17:50:22.71
CBV.dial SSV @17:50:23.48 Allowing 185s for 25.0ml of remaining 725.0ml @17:50:23.49 Intake.select :open @17:50:25.64 Intake @34.6psia, vacuum from 20.0 to 24.0psi @17:50:25.65 SS.reconfigure forSampling @17:50:25.71 SS.seek 25.00ml @17:51:05.60 Stroke completed -- waiting for pressure to equalize ... @17:51:06.66 Intake.select :close @17:51:06.81 SSV.dial :exhaust @17:51:07.57 Exhaust.select :open @17:51:07.68 SS.reconfigure SSexhale @17:51:07.73 SS.seek 0.00ml @17:51:16.24 Exhaust.select :close @17:51:16.34 SSV.dial CBV @17:51:16.36
CBV.dial SSV @17:51:17.15 Allowing 185s for 25.0ml of remaining 700.0ml @17:51:17.16 Intake.select :open @17:51:19.31 Intake @34.6psia, vacuum from 20.0 to 24.0psi @17:51:19.32 SS.reconfigure forSampling @17:51:19.38 SS.seek 25.00ml @17:51:59.41 Stroke completed -- waiting for pressure to equalize ... @17:52:00.64 Intake.select :close @17:52:00.78 SSV.dial :exhaust @17:52:01.55 Exhaust.select :open @17:52:01.66 SS.reconfigure SSexhale @17:52:01.71 SS.seek 0.00ml @17:52:10.27 Exhaust.select :close @17:52:10.38 SSV.dial CBV @17:52:10.40
CBV.dial SSV @17:52:11.18 Allowing 185s for 25.0ml of remaining 675.0ml @17:52:11.19 Intake.select :open @17:52:13.34 Intake @34.6psia, vacuum from 20.0 to 24.0psi @17:52:13.35 SS.reconfigure forSampling @17:52:13.41 SS.seek 25.00ml @17:52:53.35 Stroke completed -- waiting for pressure to equalize ... @17:52:54.57 Intake.select :close @17:52:54.71 SSV.dial :exhaust @17:52:55.47 Exhaust.select :open @17:52:55.58 SS.reconfigure SSexhale @17:52:55.63 SS.seek 0.00ml @17:53:04.19 Exhaust.select :close @17:53:04.31 SSV.dial CBV @17:53:04.33
CBV.dial SSV @17:53:05.11 Allowing 185s for 25.0ml of remaining 650.0ml @17:53:05.12 Intake.select :open @17:53:07.27 Intake @34.7psia, vacuum from 20.0 to 24.0psi @17:53:07.28 SS.reconfigure forSampling @17:53:07.35 SS.seek 25.00ml @17:53:47.33 Stroke completed -- waiting for pressure to equalize ... @17:53:48.56 Intake.select :close @17:53:48.70 SSV.dial :exhaust @17:53:49.45 Exhaust.select :open @17:53:49.65 SS.reconfigure SSexhale @17:53:49.70 SS.seek 0.00ml @17:53:58.38 Exhaust.select :close @17:53:58.49 SSV.dial CBV @17:53:58.51
CBV.dial SSV @17:53:59.31 Allowing 185s for 25.0ml of remaining 625.0ml @17:53:59.32 Intake.select :open @17:54:01.47 Intake @34.8psia, vacuum from 20.0 to 24.0psi @17:54:01.48 SS.reconfigure forSampling @17:54:01.54 SS.seek 25.00ml @17:54:11.32 -> Sleepy.power :extSampler; :on @17:54:11.33 I2C::Sleepy::Error in kevan -- Unknown powered device: extSampler @17:54:20.74 -> Sleepy.power :extSampler :on @17:54:20.75 SyntaxError in kevan -- compile error kevan:6: syntax error, unexpected ':', expecting $end Sleepy.power :extSampler :on ^ @17:54:41.53
Stroke completed -- waiting for pressure to equalize ... @17:54:42.75 Intake.select :close @17:54:42.90 SSV.dial :exhaust @17:54:43.65 Exhaust.select :open @17:54:43.76 SS.reconfigure SSexhale @17:54:43.81 SS.seek 0.00ml @17:54:52.39 Exhaust.select :close @17:54:52.49 SSV.dial CBV @17:54:52.51
CBV.dial SSV @17:54:53.29 Allowing 185s for 25.0ml of remaining 600.0ml @17:54:53.30 Intake.select :open @17:54:55.45 Intake @34.8psia, vacuum from 20.0 to 24.0psi @17:54:55.46 SS.reconfigure forSampling @17:54:55.52 SS.seek 25.00ml @17:55:04.48 -> Sleepy.power :extSampler, :on @17:55:04.49 I2C::Sleepy::Error in kevan -- Unknown powered device: extSampler @17:55:21.88 -> Sleepy.power :Sampler, :on @17:55:21.89 I2C::Sleepy::Error in kevan -- Unknown powered device: Sampler @17:55:36.32
Stroke completed -- waiting for pressure to equalize ... @17:55:36.96 Intake.select :close @17:55:37.11 SSV.dial :exhaust @17:55:37.86 Exhaust.select :open @17:55:37.97 SS.reconfigure SSexhale @17:55:38.02 SS.seek 0.00ml @17:55:46.61 Exhaust.select :close @17:55:46.72 SSV.dial CBV @17:55:46.74
CBV.dial SSV @17:55:47.52 Allowing 185s for 25.0ml of remaining 575.0ml @17:55:47.53 Intake.select :open @17:55:49.68 Intake @34.9psia, vacuum from 20.0 to 24.0psi @17:55:49.69 SS.reconfigure forSampling @17:55:49.75 SS.seek 25.00ml @17:56:29.72 Stroke completed -- waiting for pressure to equalize ... @17:56:30.94 Intake.select :close @17:56:31.08 SSV.dial :exhaust @17:56:31.86 Exhaust.select :open @17:56:31.99 SS.reconfigure SSexhale @17:56:32.04 SS.seek 0.00ml @17:56:40.61 Exhaust.select :close @17:56:40.72 SSV.dial CBV @17:56:40.74
CBV.dial SSV @17:56:41.52 Allowing 185s for 25.0ml of remaining 550.0ml @17:56:41.53 Intake.select :open @17:56:43.86 Intake @34.8psia, vacuum from 20.0 to 24.0psi @17:56:43.87 SS.reconfigure forSampling @17:56:43.93 SS.seek 25.00ml @17:56:55.63 -> Sleepy.power :sampler, :on @17:56:55.64 Sleepy.power :sampler,:ON @17:57:19.54 -> Sleepy.power :sampler, :off @17:57:19.55 Sleepy.power :sampler,:OFF @17:57:23.86
Stroke completed -- waiting for pressure to equalize ... @17:57:24.62 -> exit @17:57:24.63 Session Ended @17:57:25.08
Intake.select :close @17:57:25.21 SSV.dial :exhaust @17:57:25.97 Exhaust.select :open @17:57:26.07 SS.reconfigure SSexhale @17:57:26.11 SS.seek 0.00ml @17:57:34.69 Exhaust.select :close @17:57:34.78 SSV.dial CBV @17:57:34.80
CBV.dial SSV @17:57:35.56 Allowing 185s for 25.0ml of remaining 525.0ml @17:57:35.57 Intake.select :open @17:57:37.70 Intake @34.8psia, vacuum from 20.0 to 24.0psi @17:57:37.71 SS.reconfigure forSampling @17:57:37.75 SS.seek 25.00ml @17:58:17.73 Stroke completed -- waiting for pressure to equalize ... @17:58:18.96 Intake.select :close @17:58:19.09 SSV.dial :exhaust @17:58:19.84 Exhaust.select :open @17:58:19.93 SS.reconfigure SSexhale @17:58:19.97 SS.seek 0.00ml @17:58:28.37 Exhaust.select :close @17:58:28.46 SSV.dial CBV @17:58:28.48
CBV.dial SSV @17:58:29.25 Allowing 185s for 25.0ml of remaining 500.0ml @17:58:29.26 Intake.select :open @17:58:31.39 Intake @34.9psia, vacuum from 20.0 to 24.0psi @17:58:31.40 SS.reconfigure forSampling @17:58:31.44 SS.seek 25.00ml @17:58:52.79 Vacuum too high -- waiting up to 40s @17:58:53.05 Intake @33.4psia, vacuum from 20.0 to 24.0psi @17:58:53.06 SS.reconfigure forSampling @17:58:53.10 SS.seek 25.00ml @17:59:21.01 Stroke completed -- waiting for pressure to equalize ... @17:59:22.23 Intake.select :close @17:59:22.37 SSV.dial :exhaust @17:59:23.12 Exhaust.select :open @17:59:23.21 SS.reconfigure SSexhale @17:59:23.25 SS.seek 0.00ml @17:59:31.84 Exhaust.select :close @17:59:31.93 SSV.dial CBV @17:59:31.95
CBV.dial SSV @17:59:32.71 Allowing 185s for 25.0ml of remaining 475.0ml @17:59:32.72 Intake.select :open @17:59:34.85 Intake @34.9psia, vacuum from 20.0 to 24.0psi @17:59:34.86 SS.reconfigure forSampling @17:59:34.90 SS.seek 25.00ml @18:00:34.27 Stroke completed -- waiting for pressure to equalize ... @18:00:35.48 Intake.select :close @18:00:35.62 SSV.dial :exhaust @18:00:36.38 Exhaust.select :open @18:00:36.47 SS.reconfigure SSexhale @18:00:36.51 SS.seek 0.00ml @18:00:45.02 Exhaust.select :close @18:00:45.11 SSV.dial CBV @18:00:45.13
CBV.dial SSV @18:00:45.89 Allowing 185s for 25.0ml of remaining 450.0ml @18:00:45.90 Intake.select :open @18:00:48.03 Intake @34.8psia, vacuum from 20.0 to 24.0psi @18:00:48.04 SS.reconfigure forSampling @18:00:48.08 SS.seek 25.00ml @18:01:47.47 Stroke completed -- waiting for pressure to equalize ... @18:01:48.69 Intake.select :close @18:01:48.82 SSV.dial :exhaust @18:01:49.58 Exhaust.select :open @18:01:49.67 SS.reconfigure SSexhale @18:01:49.71 SS.seek 0.00ml @18:01:58.45 Exhaust.select :close @18:01:58.54 SSV.dial CBV @18:01:58.56
CBV.dial SSV @18:01:59.33 Allowing 185s for 25.0ml of remaining 425.0ml @18:01:59.34 Intake.select :open @18:02:01.47 Intake @35.0psia, vacuum from 20.0 to 24.0psi @18:02:01.48 SS.reconfigure forSampling @18:02:01.52 SS.seek 25.00ml @18:03:01.45 Stroke completed -- waiting for pressure to equalize ... @18:03:02.09 Intake.select :close @18:03:02.22 SSV.dial :exhaust @18:03:02.97 Exhaust.select :open @18:03:03.06 SS.reconfigure SSexhale @18:03:03.10 SS.seek 0.00ml @18:03:11.73 Exhaust.select :close @18:03:11.82 SSV.dial CBV @18:03:11.84
CBV.dial SSV @18:03:12.61 Allowing 185s for 25.0ml of remaining 400.0ml @18:03:12.62 Intake.select :open @18:03:14.86 Intake @34.9psia, vacuum from 20.0 to 24.0psi @18:03:14.87 SS.reconfigure forSampling @18:03:15.43 SS.seek 25.00ml @18:04:14.85 Stroke completed -- waiting for pressure to equalize ... @18:04:16.08 Intake.select :close @18:04:16.22 SSV.dial :exhaust @18:04:16.98 Exhaust.select :open @18:04:17.08 SS.reconfigure SSexhale @18:04:17.12 SS.seek 0.00ml @18:04:25.68 Exhaust.select :close @18:04:25.77 SSV.dial CBV @18:04:25.79
CBV.dial SSV @18:04:26.55 Allowing 185s for 25.0ml of remaining 375.0ml @18:04:26.56 Intake.select :open @18:04:28.69 Intake @34.8psia, vacuum from 20.0 to 24.0psi @18:04:28.70 SS.reconfigure forSampling @18:04:28.74 SS.seek 25.00ml @18:05:28.13 Stroke completed -- waiting for pressure to equalize ... @18:05:29.36 Intake.select :close @18:05:29.49 SSV.dial :exhaust @18:05:30.24 Exhaust.select :open @18:05:30.35 SS.reconfigure SSexhale @18:05:30.43 SS.seek 0.00ml @18:05:39.00 Exhaust.select :close @18:05:39.09 SSV.dial CBV @18:05:39.11
CBV.dial SSV @18:05:39.88 Allowing 185s for 25.0ml of remaining 350.0ml @18:05:39.89 Intake.select :open @18:05:42.02 Intake @34.9psia, vacuum from 20.0 to 24.0psi @18:05:42.03 SS.reconfigure forSampling @18:05:42.07 SS.seek 25.00ml @18:06:41.45 Stroke completed -- waiting for pressure to equalize ... @18:06:42.68 Intake.select :close @18:06:42.82 SSV.dial :exhaust @18:06:43.58 Exhaust.select :open @18:06:43.67 SS.reconfigure SSexhale @18:06:43.71 SS.seek 0.00ml @18:06:52.30 Exhaust.select :close @18:06:52.39 SSV.dial CBV @18:06:52.41
CBV.dial SSV @18:06:53.17 Allowing 185s for 25.0ml of remaining 325.0ml @18:06:53.18 Intake.select :open @18:06:55.33 Intake @34.9psia, vacuum from 20.0 to 24.0psi @18:06:55.34 SS.reconfigure forSampling @18:06:55.38 SS.seek 25.00ml @18:07:55.43 Stroke completed -- waiting for pressure to equalize ... @18:07:56.07 Intake.select :close @18:07:56.20 SSV.dial :exhaust @18:07:56.95 Exhaust.select :open @18:07:57.05 SS.reconfigure SSexhale @18:07:57.09 SS.seek 0.00ml @18:08:05.50 Exhaust.select :close @18:08:05.59 SSV.dial CBV @18:08:05.61
CBV.dial SSV @18:08:06.37 Allowing 185s for 25.0ml of remaining 300.0ml @18:08:06.38 Intake.select :open @18:08:08.53 Intake @34.8psia, vacuum from 20.0 to 24.0psi @18:08:08.54 SS.reconfigure forSampling @18:08:08.58 SS.seek 25.00ml @18:09:08.71 Stroke completed -- waiting for pressure to equalize ... @18:09:09.35 Intake.select :close @18:09:09.48 SSV.dial :exhaust @18:09:10.23 Exhaust.select :open @18:09:10.34 SS.reconfigure SSexhale @18:09:10.37 SS.seek 0.00ml @18:09:18.91 Exhaust.select :close @18:09:19.00 SSV.dial CBV @18:09:19.02
CBV.dial SSV @18:09:19.79 Allowing 185s for 25.0ml of remaining 275.0ml @18:09:19.80 Intake.select :open @18:09:21.93 Intake @34.8psia, vacuum from 20.0 to 24.0psi @18:09:21.94 SS.reconfigure forSampling @18:09:21.98 SS.seek 25.00ml @18:10:21.19 Stroke completed -- waiting for pressure to equalize ... @18:10:22.41 Intake.select :close @18:10:22.54 SSV.dial :exhaust @18:10:23.29 Exhaust.select :open @18:10:23.39 SS.reconfigure SSexhale @18:10:23.43 SS.seek 0.00ml @18:10:31.85 Exhaust.select :close @18:10:31.94 SSV.dial CBV @18:10:31.96
CBV.dial SSV @18:10:32.72 Allowing 185s for 25.0ml of remaining 250.0ml @18:10:32.73 Intake.select :open @18:10:34.86 Intake @34.8psia, vacuum from 20.0 to 24.0psi @18:10:34.87 SS.reconfigure forSampling @18:10:34.91 SS.seek 25.00ml @18:11:34.28 Stroke completed -- waiting for pressure to equalize ... @18:11:35.49 Intake.select :close @18:11:35.63 SSV.dial :exhaust @18:11:36.38 Exhaust.select :open @18:11:36.48 SS.reconfigure SSexhale @18:11:36.52 SS.seek 0.00ml @18:11:45.02 Exhaust.select :close @18:11:45.11 SSV.dial CBV @18:11:45.13
CBV.dial SSV @18:11:45.91 Allowing 185s for 25.0ml of remaining 225.0ml @18:11:45.92 Intake.select :open @18:11:48.05 Intake @34.8psia, vacuum from 20.0 to 24.0psi @18:11:48.06 SS.reconfigure forSampling @18:11:48.10 SS.seek 25.00ml @18:12:48.19 Stroke completed -- waiting for pressure to equalize ... @18:12:48.83 Intake.select :close @18:12:48.96 SSV.dial :exhaust @18:12:49.71 Exhaust.select :open @18:12:49.80 SS.reconfigure SSexhale @18:12:49.84 SS.seek 0.00ml @18:12:58.38 Exhaust.select :close @18:12:58.47 SSV.dial CBV @18:12:58.49
CBV.dial SSV @18:12:59.26 Allowing 185s for 25.0ml of remaining 200.0ml @18:12:59.27 Intake.select :open @18:13:01.82 Intake @34.9psia, vacuum from 20.0 to 24.0psi @18:13:01.83 SS.reconfigure forSampling @18:13:01.86 SS.seek 25.00ml @18:14:01.24 Stroke completed -- waiting for pressure to equalize ... @18:14:02.46 Intake.select :close @18:14:02.60 SSV.dial :exhaust @18:14:03.36 Exhaust.select :open @18:14:03.46 SS.reconfigure SSexhale @18:14:03.50 SS.seek 0.00ml @18:14:11.94 Exhaust.select :close @18:14:12.03 SSV.dial CBV @18:14:12.05
CBV.dial SSV @18:14:12.81 Allowing 185s for 25.0ml of remaining 175.0ml @18:14:12.82 Intake.select :open @18:14:14.95 Intake @34.8psia, vacuum from 20.0 to 24.0psi @18:14:14.96 SS.reconfigure forSampling @18:14:15.00 SS.seek 25.00ml @18:15:14.42 Stroke completed -- waiting for pressure to equalize ... @18:15:15.65 Intake.select :close @18:15:15.80 SSV.dial :exhaust @18:15:16.55 Exhaust.select :open @18:15:16.64 SS.reconfigure SSexhale @18:15:16.68 SS.seek 0.00ml @18:15:25.36 Exhaust.select :close @18:15:25.45 SSV.dial CBV @18:15:25.47
CBV.dial SSV @18:15:26.23 Allowing 185s for 25.0ml of remaining 150.0ml @18:15:26.24 Intake.select :open @18:15:28.37 Intake @34.8psia, vacuum from 20.0 to 24.0psi @18:15:28.38 SS.reconfigure forSampling @18:15:28.42 SS.seek 25.00ml @18:16:27.74 Stroke completed -- waiting for pressure to equalize ... @18:16:28.96 Intake.select :close @18:16:29.10 SSV.dial :exhaust @18:16:29.85 Exhaust.select :open @18:16:29.95 SS.reconfigure SSexhale @18:16:29.99 SS.seek 0.00ml @18:16:38.73 Exhaust.select :close @18:16:38.82 SSV.dial CBV @18:16:38.84
CBV.dial SSV @18:16:39.61 Allowing 185s for 25.0ml of remaining 125.0ml Intake.select :open @18:16:41.74 Intake @34.8psia, vacuum from 20.0 to 24.0psi @18:16:41.75 SS.reconfigure forSampling @18:16:41.79 SS.seek 25.00ml @18:17:41.04 Stroke completed -- waiting for pressure to equalize ... @18:17:42.26 Intake.select :close @18:17:42.40 SSV.dial :exhaust @18:17:43.15 Exhaust.select :open @18:17:43.24 SS.reconfigure SSexhale @18:17:43.28 SS.seek 0.00ml @18:17:51.80 Exhaust.select :close @18:17:51.90 SSV.dial CBV @18:17:51.92
CBV.dial SSV @18:17:52.69 Allowing 185s for 25.0ml of remaining 100.0ml @18:17:52.70 Intake.select :open @18:17:54.83 Intake @34.8psia, vacuum from 20.0 to 24.0psi @18:17:54.84 SS.reconfigure forSampling @18:17:54.88 SS.seek 25.00ml @18:18:54.24 Stroke completed -- waiting for pressure to equalize ... @18:18:55.45 Intake.select :close @18:18:55.59 SSV.dial :exhaust @18:18:56.35 Exhaust.select :open @18:18:56.44 SS.reconfigure SSexhale @18:18:56.48 SS.seek 0.00ml @18:19:04.01 Session Begun @18:19:05.06
Exhaust.select :close @18:19:05.16 SSV.dial CBV @18:19:05.18
CBV.dial SSV @18:19:05.97 Allowing 185s for 25.0ml of remaining 75.0ml @18:19:05.98 Intake.select :open @18:19:08.22 Intake @34.8psia, vacuum from 20.0 to 24.0psi @18:19:08.23 SS.reconfigure forSampling @18:19:08.30 SS.seek 25.00ml @18:20:07.75 Stroke completed -- waiting for pressure to equalize ... @18:20:08.98 Intake.select :close @18:20:09.13 SSV.dial :exhaust @18:20:09.89 Exhaust.select :open @18:20:10.00 SS.reconfigure SSexhale @18:20:10.05 SS.seek 0.00ml @18:20:18.40 Exhaust.select :close @18:20:18.51 SSV.dial CBV @18:20:18.53
CBV.dial SSV @18:20:19.32 Allowing 185s for 25.0ml of remaining 50.0ml @18:20:19.33 Intake.select :open @18:20:21.48 Intake @34.8psia, vacuum from 20.0 to 24.0psi @18:20:21.49 SS.reconfigure forSampling @18:20:21.55 SS.seek 25.00ml @18:21:20.96 Stroke completed -- waiting for pressure to equalize ... @18:21:22.29 Intake.select :close @18:21:22.44 SSV.dial :exhaust @18:21:23.20 Exhaust.select :open @18:21:23.31 SS.reconfigure SSexhale @18:21:23.36 SS.seek 0.00ml @18:21:31.93 Exhaust.select :close @18:21:32.05 SSV.dial CBV @18:21:32.07
CBV.dial SSV @18:21:32.85 Allowing 185s for 25.0ml of remaining 25.0ml @18:21:32.86 Intake.select :open @18:21:35.01 Intake @34.9psia, vacuum from 20.0 to 24.0psi @18:21:35.02 SS.reconfigure forSampling @18:21:35.08 SS.seek 24.97ml @18:22:34.26 Stroke completed -- waiting for pressure to equalize ... @18:22:35.48 Intake.select :close @18:22:35.62 SSV.dial :exhaust @18:22:36.38 Exhaust.select :open @18:22:36.92 SS.reconfigure SSexhale @18:22:36.97 SS.seek 0.00ml @18:22:45.68 Exhaust.select :close @18:22:45.79 Sampled 1000.0ml @18:22:45.80 collectSample: Complete @18:22:45.81 Evacuating Puck (bubblePt=55.0psi, delay=15.0s) @18:22:45.82 Intake.select :close @18:22:45.90 CSV.dial :waste @18:22:45.93 CS.seek 0.000ml @18:22:46.02 SSV.dial CBV,avoiding => exhaust @18:22:46.05
CBV.dial SSV @18:22:46.11 CSV.dial :air @18:22:46.83 CS.seek 10.000ml @18:22:47.89
CTV.dial :air @18:22:48.64 delay 15 seconds @18:23:03.64 SSV.dial :spit,avoiding => exhaust @18:23:04.97 CTV.dial CSV,avoiding => intake @18:23:04.99
SS.seek 0.00ml @18:23:05.10 SSV.dial CBV,avoiding => exhaust @18:23:10.31 CBV.dial between CSV and waste2b @18:23:11.26 CSV.dial between air and CTV @18:23:11.58 CS.seek 8.443ml @18:23:16.05 CTV.dial CSV,avoiding => air @18:23:16.06
CSV.dial CTV @18:23:16.38 CS.reconfigure CSpressurize @18:23:16.42 CS.seek 0.000ml @18:23:36.93 BubblePoint: Intake @70.1psia, Outlet @15.0psia @18:23:36.94 SS.reconfigure SSconfig @18:23:37.00 SS.seek 10.00ml @18:23:42.97 delay 15 seconds @18:23:58.07 Evacuated: Intake @69.0psia, Outlet @-0.1psia @18:23:58.08 SSV.dial :spit,avoiding => exhaust @18:23:59.39 SS.reconfigure SSexhale @18:23:59.43 SS.seek 0.00ml @18:24:04.72 SS.reconfigure SSfast @18:24:04.77 SS.seek 5.00ml @18:24:07.62 SSV.dial CBV,avoiding => exhaust @18:24:07.64
CBV.dial SSV @18:24:09.00 CTV.dial :air,avoiding => intake @18:24:10.31 CSV.dial CTV @18:24:10.32
SS.reconfigure SSconfig @18:24:10.38 SS.seek 10.00ml @18:24:11.32 CSV.dial :air @18:24:11.84 CS.reconfigure CSfast @18:24:11.89 CS.seek 0.000ml @18:24:13.50
SSV.dial :spit,avoiding => exhaust @18:24:14.13 CS.coast @18:24:14.82
SS.reconfigure SSexhale @18:24:14.87 SS.seek 0.00ml @18:24:20.09 SS.reconfigure SSfast @18:24:20.13 SS.seek 5.00ml @18:24:23.12 SSV.dial CBV,avoiding => exhaust @18:24:23.14
CBV.dial SSV @18:24:25.44 SSV.dial :spit,avoiding => exhaust @18:24:26.74 SS.reconfigure SSconfig @18:24:26.78 SS.seek 0.00ml @18:24:30.06 SS.coast @18:24:30.10 Evacuating Puck (bubblePt=55.0psi, delay=15.0s) @18:24:30.11 Intake.select :close @18:24:30.18 CSV.dial :waste @18:24:30.27
SSV.dial :exhaust @18:24:30.94 CS.seek 0.000ml @18:24:31.00 CSV.dial :air @18:24:31.06
Exhaust.select :open @18:24:31.17 SS.reconfigure SSexhale @18:24:31.22 SS.seek 0.00ml @18:24:31.66 Exhaust.select :close @18:24:31.76 SSV.dial CBV,avoiding => exhaust @18:24:31.78
CBV.dial SSV @18:24:31.84 CS.seek 10.000ml @18:24:33.65
CTV.dial :air @18:24:33.66 delay 15 seconds @18:24:48.67 SSV.dial :spit,avoiding => exhaust @18:24:49.99 CTV.dial CSV,avoiding => intake @18:24:50.01
SS.seek 0.00ml @18:24:50.12 SSV.dial CBV,avoiding => exhaust @18:24:55.24 CBV.dial between CSV and waste2b @18:24:56.19 CSV.dial between air and CTV @18:24:56.50 CS.seek 7.447ml @18:25:03.11 CTV.dial CSV,avoiding => air @18:25:03.12
CSV.dial CTV @18:25:03.65 CS.reconfigure CSpressurize @18:25:03.69 CS.seek 0.000ml @18:25:23.10 CSV.dial :air @18:25:23.54 CS.reconfigure CSfast @18:25:23.58 CS.seek 10.000ml @18:25:46.77 CSV.dial between air and CTV @18:25:47.17 CS.seek 2.855ml @18:25:58.11 Session Begun @18:26:04.17 CTV.dial CSV,avoiding => air @18:26:04.19
CSV.dial CTV @18:26:04.51 CS.reconfigure CSpressurize @18:26:04.56 CS.seek 0.000ml @18:26:10.36 BubblePoint: Intake @70.6psia, Outlet @15.3psia @18:26:10.37 SS.reconfigure SSconfig @18:26:10.44 SS.seek 10.00ml @18:26:16.43 delay 15 seconds @18:26:31.53 Evacuated: Intake @69.5psia, Outlet @-0.1psia @18:26:31.54 SSV.dial :spit,avoiding => exhaust @18:26:32.88 SS.reconfigure SSexhale @18:26:32.93 SS.seek 0.00ml @18:26:37.90 SS.reconfigure SSfast @18:26:37.96 SS.seek 5.00ml @18:26:41.14 SSV.dial CBV,avoiding => exhaust @18:26:41.16
CBV.dial SSV @18:26:42.52 CTV.dial :air,avoiding => intake @18:26:43.85 CSV.dial CTV @18:26:43.87
SS.reconfigure SSconfig @18:26:43.94 SS.seek 10.00ml @18:26:44.86 CSV.dial :air @18:26:45.40 CS.reconfigure CSfast @18:26:45.45 CS.seek 0.000ml @18:26:47.10
SSV.dial :spit,avoiding => exhaust @18:26:48.06 CS.coast @18:26:48.44
SS.reconfigure SSexhale @18:26:48.49 SS.seek 0.00ml @18:26:53.71 SS.reconfigure SSfast @18:26:53.77 SS.seek 5.00ml @18:26:56.77 SSV.dial CBV,avoiding => exhaust @18:26:56.79
CBV.dial SSV @18:26:59.09 SSV.dial :spit,avoiding => exhaust @18:27:00.41 SS.reconfigure SSconfig @18:27:00.46 SS.seek 0.00ml @18:27:03.77 SS.coast @18:27:03.82 Lysing: Adding 1.5ml lysis @18:27:03.83 CBV.dial between CSV and waste1,avoiding => DBV @18:27:05.33 CTV.dial CSV @18:27:06.59 CSV.dial CSR @18:27:07.40 CSR.select :lysis @18:27:07.52 CS.reconfigure CSconfig @18:27:07.57 CS.seek 1.000ml @18:27:10.90 delay 5 seconds @18:27:15.91 CSR.select :air @18:27:16.07 CS.seek 5.999ml @18:27:29.32 -> exit @18:27:29.33 Session Ended @18:27:29.53
delay 2 seconds @18:27:31.54 CSV.dial :waste1 @18:27:32.82 CS.reconfigure CSfast @18:27:32.86 CS.seek empty @18:27:47.18 CSV.dial CSR @18:27:48.46 CSR.select :lysis @18:27:48.57 CS.reconfigure CSconfig @18:27:48.62 CS.seek 1.500ml @18:27:53.24 delay 5 seconds @18:27:58.26 CSR.select :air @18:27:58.42 CS.seek 4.500ml @18:28:01.72 -> exit @18:28:01.73 Session Ended @18:28:06.90
delay 2 seconds @18:28:08.91 CSV.dial CTV @18:28:09.43 CS.seek 1.275ml @18:28:18.44 Heating sh1 puck from tube 2 in Collection Clamp to 85.0C for 5:00 @18:28:18.45 CH.seek 85.0C,8:00 @18:30:12.35 CH.hold 5:00,85.0C @18:35:12.46 Collection Puck Heater at 85.0C after lysing @18:35:12.47 CS.seek 0.000ml @18:35:16.53 CTV.dial between spare and air,avoiding => intake @18:35:17.52 CSV.dial :air @18:35:17.98 CS.seek 5.000ml @18:35:31.55 CSV.dial CTV @18:35:32.06 delay 2 seconds @18:35:34.07 CSV.dial :air @18:35:34.59 delay 2 seconds @18:35:36.60 CSV.dial CTV @18:35:37.31 delay 2 seconds @18:35:39.32 CSV.dial :air @18:35:39.75 delay 2 seconds @18:35:41.76 CS.seek 0.000ml @18:35:55.45 Recovering Lysate into Collection syringe @18:35:55.46 CSV.dial CBV @18:35:56.45 CBV.dial CSV @18:35:56.80 CS.seek 5.000ml @18:36:10.23 delay 5 seconds @18:36:15.24 CTV.dial :air @18:36:15.70 CS.seek 7.000ml @18:36:21.64 delay 5 seconds @18:36:26.65 CSV.dial :air2 @18:36:27.08 CS.seek 10.000ml @18:36:35.54 CSV.dial between air and CTV,avoiding => CBV @18:36:36.88 Lysing Complete @18:36:36.93 Storing sh1 puck in Collection Clamp to tube 1 @18:36:37.05 SC.reconfigure SCtight @18:36:37.08 SC.seek 1 @18:36:39.36 SC.reconfigure SCloose @18:36:39.52 Elbow.reconfigure TightElbow @18:36:39.56 Elbow.seek 1 @18:36:41.19 Forearm.reconfigure TightForearm @18:36:41.22 Forearm.seek 1 + 800 counts @18:36:42.29 SE.reconfigure SEpick @18:36:42.34 SE.seek beamBreak @18:36:45.13 SE.configure SEconfig @18:36:45.17 SE.seek place @18:36:45.65 Forearm.reconfigure LooseForearm @18:36:45.68 Forearm.seek retract @18:36:46.60 Elbow.seek CC @18:36:48.17 Elbow.reconfigure LooseElbow @18:36:48.25 Forearm.seek CC @18:36:49.19 CC.seek sealed @18:36:50.10 Hand.grip @18:36:50.84 CC.seek open @18:36:54.17 Forearm.seek retract @18:36:55.68 Elbow.reconfigure TightElbow @18:36:55.71 Elbow.seek 1 @18:36:57.28 Elbow.reconfigure LimpElbow @18:36:57.33 Forearm.seek 1 @18:36:58.38 Elbow.hold @18:36:58.43 Elbow.reconfigure TightElbow @18:36:58.47 Hand.release @18:36:59.30 Forearm.coast @18:36:59.33 Forearm.seek 1 + 318 counts @18:36:59.56 Forearm.reconfigure TightForearm @18:36:59.59 Forearm.seek 1 + 800 counts @18:36:59.92 SE.seek down @18:37:01.02
Forearm.reconfigure LooseForearm @18:37:01.06 Forearm.seek retract @18:37:02.62 SE.coast @18:37:02.65
SC.coast @18:37:02.68 Tranferring lysate to PRV CSV.dial :air @18:37:03.07 CS.seek 10.000ml @18:37:03.13 CSV.dial between air and CTV @18:37:04.13 AIV.dial :bypass @18:37:04.19 Beginning samplePassC @18:37:04.20 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @18:37:04.34 Elbow.seek FlushPuck::Garage @18:37:06.10 Elbow.reconfigure LooseElbow @18:37:06.18 Forearm.seek garage @18:37:07.19 Hand.grip @18:37:08.06 Forearm.seek retract @18:37:09.04 Elbow.reconfigure TightElbow @18:37:09.08 Elbow.seek CC @18:37:11.89 Elbow.reconfigure LooseElbow @18:37:11.98 Forearm.reconfigure TightForearm @18:37:12.01 Forearm.seek CC @18:37:13.36 CC.seek closed @18:37:17.12 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 2.01 @18:37:17.17 Hand.release @18:37:18.01 Forearm.reconfigure LooseForearm @18:37:18.04 Forearm.seek retract @18:37:19.02 AWV.select :air @18:37:19.03 AIV.dial :bypass @18:37:19.04 PSV.dial PSR @18:37:20.32 PSR.select :flush @18:37:20.42 PS.seek 1.500ml @18:37:36.55 delay 5 seconds @18:37:41.57 PSV.dial :waste1 @18:37:42.02 PS.seek 0.000ml @18:37:58.08 PSV.dial PSR @18:37:58.54 PSR.select :air @18:37:58.64 PS.seek 2.500ml @18:38:24.94 CBV.dial PRV @18:38:24.95 PRV.dial CBV @18:38:25.73
PSV.dial :mixloop @18:38:26.72 CTV.dial :air,avoiding => intake @18:38:26.73 PTV.dial :PRVmixing @18:38:26.81 PS.seek 0.000ml @18:38:52.99 PSV.dial :air @18:38:53.43 PS.seek 2.500ml @18:39:19.64 PSV.dial :mixloop @18:39:20.09 PS.seek 0.000ml @18:39:46.39 PSV.dial :air @18:39:46.84 PS.seek 2.500ml @18:40:13.05 PSV.dial :mixloop @18:40:13.49 PS.seek 0.000ml @18:40:39.73 Garaging FlushPuck in Collection Clamp @18:40:39.79 Elbow.reconfigure TightElbow @18:40:39.82 Elbow.seek CC @18:40:40.02 Elbow.reconfigure LooseElbow @18:40:40.11 Forearm.seek CC @18:40:41.07 CC.seek sealed @18:40:42.00 Hand.grip @18:40:42.71 CC.seek open @18:40:46.52 Forearm.seek retract @18:40:47.46 Elbow.reconfigure TightElbow @18:40:47.50 Elbow.seek garage @18:40:50.30 Elbow.reconfigure LooseElbow @18:40:50.38 Forearm.reconfigure TightForearm @18:40:50.41 Forearm.seek garage @18:40:51.57 Hand.release @18:40:52.37 Forearm.reconfigure LooseForearm @18:40:52.41 Forearm.seek retract @18:40:53.45 Connecting PS to CS to transfer sample @18:40:53.46 CBV.dial AIV @18:40:53.47 AIV.dial :bypass @18:40:53.48 PSV.dial :mixloop @18:40:53.49 PTV.dial :PRVmixing @18:40:53.50 PRV.dial AIV @18:40:53.51 CTV.dial :air,avoiding => intake @18:40:53.52 Loading lyfil puck from tube 2 into Collection Clamp @18:40:53.69 SC.reconfigure SCtight @18:40:53.72 SC.seek 2 @18:40:56.00 SC.reconfigure SCloose @18:40:56.17 Elbow.reconfigure TightElbow @18:40:56.21 Elbow.seek 2 @18:40:58.02 Forearm.reconfigure TightForearm @18:40:58.05 Forearm.seek 2 + 800 counts @18:40:59.14 SE.reconfigure SEpick @18:40:59.19 SE.seek beamBreak @18:41:00.13 SE.configure SEconfig @18:41:00.25 Elbow.seek 2 @18:41:00.39 Forearm.seek 2 + 300 counts @18:41:00.67 Forearm.reconfigure LooseForearm @18:41:00.71 Hand.grip @18:41:01.47 SE.seek down @18:41:01.61
Forearm.seek retract @18:41:02.28 SE.coast @18:41:02.55
Elbow.seek CC @18:41:04.17 Elbow.reconfigure LooseElbow @18:41:04.25 Forearm.reconfigure TightForearm @18:41:04.28 Forearm.seek CC @18:41:05.44 CC.seek closed @18:41:09.20 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.99 @18:41:09.21 Hand.release @18:41:10.00 Forearm.reconfigure LooseForearm @18:41:10.03 Forearm.seek retract @18:41:10.95 SC.coast @18:41:10.97 CTV.dial CSV,avoiding => intake @18:41:12.26 CSV.dial CTV @18:41:12.65 CS.seek 5.000ml @18:41:26.06 delay 1:20 @18:42:46.07 CS.seek 2.500ml @18:42:53.24 delay 40 seconds @18:43:33.25 CS.seek 0.000ml @18:43:40.37 delay 1:00 @18:44:40.38 PSV.dial :air @18:44:40.84 PS.seek 2.500ml @18:45:07.13 PSV.dial :mixloop @18:45:07.57 delay 3 seconds @18:45:10.58 PSV.dial :air @18:45:11.02 delay 2 seconds @18:45:13.03 PSV.dial :mixloop @18:45:13.47 delay 3 seconds @18:45:16.48 PSV.dial :waste1 @18:45:17.76 PS.seek empty @18:45:43.97 CBV.dial :waste1 @18:45:44.42 delay 5 seconds @18:45:49.43 CBV.dial AIV @18:45:49.88 delay 10 seconds @18:45:59.89 CBV.dial :waste1 @18:46:00.36 delay 10 seconds @18:46:10.37 CBV.dial AIV @18:46:10.83 delay 10 seconds @18:46:20.84 CTV.dial CSV,avoiding => intake @18:46:20.85 CBV.dial between AIV and waste1 @18:46:21.17 CSV.dial :air @18:46:21.70 CS.seek 10.000ml @18:46:47.80 CSV.dial CTV @18:46:48.33 CS.seek 0.000ml @18:47:14.46 CBV.dial :waste1 @18:47:14.76 delay 10 seconds @18:47:24.77 CTV.dial :air,avoiding => intake @18:47:26.12 Storing lyfil puck in Collection Clamp to tube 1 @18:47:26.24 SC.reconfigure SCtight @18:47:26.27 SC.seek 1 @18:47:28.59 SC.reconfigure SCloose @18:47:28.75 Elbow.reconfigure TightElbow @18:47:28.79 Elbow.seek 1 @18:47:30.87 Forearm.reconfigure TightForearm @18:47:30.90 Forearm.seek 1 + 800 counts @18:47:32.01 SE.reconfigure SEpick @18:47:32.06 SE.seek beamBreak @18:47:34.97 SE.configure SEconfig @18:47:35.09 Elbow.reconfigure LimpElbow @18:47:35.12 Forearm.reconfigure LooseForearm @18:47:35.17 Forearm.seek 1 @18:47:35.42 Elbow.hold @18:47:35.47 Elbow.reconfigure TightElbow @18:47:35.56 SE.seek 0.04 Pucks below Top Plate @18:47:35.85 Forearm.coast @18:47:35.88 Forearm.seek 1 + 337 counts @18:47:36.12 Forearm.reconfigure TightForearm @18:47:36.15 Forearm.seek 1 + 800 counts @18:47:36.42 SE.seek 1.04 Pucks below Top Plate @18:47:36.97 Forearm.reconfigure LooseForearm @18:47:37.00 Forearm.seek retract @18:47:37.95 Elbow.seek CC @18:47:39.59 Elbow.reconfigure LooseElbow @18:47:39.68 Forearm.seek CC @18:47:40.62 CC.seek sealed @18:47:41.55 Hand.grip @18:47:42.32 CC.seek open @18:47:42.70 Session Begun @18:47:45.66
Forearm.seek retract @18:47:46.61 Elbow.reconfigure TightElbow @18:47:46.66 Elbow.seek 1 @18:47:48.26 Elbow.reconfigure LimpElbow @18:47:48.32 Forearm.seek 1 @18:47:49.36 Elbow.hold @18:47:49.42 Elbow.reconfigure TightElbow @18:47:49.47 Hand.release @18:47:50.28 Forearm.coast @18:47:50.32 Forearm.seek 1 + 319 counts @18:47:50.57 Forearm.reconfigure TightForearm @18:47:50.62 Forearm.seek 1 + 800 counts @18:47:50.93 SE.seek down @18:47:52.03
Forearm.reconfigure LooseForearm @18:47:52.08 Forearm.seek retract @18:47:53.54 SE.coast @18:47:53.58
SC.coast @18:47:53.62 PSV.dial :air @18:47:54.68 PS.seek 0.499ml @18:48:00.72 delay 3 seconds @18:48:03.73 PSV.dial :mixloop @18:48:04.18 PRV.dial :air @18:48:04.63 delay 3 seconds @18:48:07.64 PRV.dial CBV @18:48:08.11 CBV.dial AIV @18:48:08.57 PRV.dial between CBV and air @18:48:08.90 PRV.dial :air @18:48:09.26 PS.reconfigure PSslow @18:48:09.30 PS.seek 0.599ml @18:48:11.82 PRV.dial :waste2 @18:48:12.55 PS.reconfigure PSconfig @18:48:12.59 PS.seek empty @18:48:19.50 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @18:48:19.66 Elbow.seek FlushPuck::Garage @18:48:21.43 Elbow.reconfigure LooseElbow @18:48:21.53 Forearm.seek garage @18:48:22.59 Hand.grip @18:48:23.41 Forearm.seek retract @18:48:24.44 Elbow.reconfigure TightElbow @18:48:24.49 Elbow.seek CC @18:48:27.21 Elbow.reconfigure LooseElbow @18:48:27.31 Forearm.reconfigure TightForearm @18:48:27.35 Forearm.seek CC @18:48:28.48 CC.seek closed @18:48:32.26 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 2.06 @18:48:32.33 Hand.release @18:48:33.12 Forearm.reconfigure LooseForearm @18:48:33.16 Forearm.seek retract @18:48:34.21 Clearing PML for modified lysate @18:48:34.22 CTV.dial :air,avoiding => intake @18:48:34.24 PRV.dial :waste2 @18:48:34.25 PTV.dial :PRV,avoiding => puck @18:48:35.00 delay 2 seconds @18:48:37.00 PTV.dial :PRVmixing,avoiding => puck @18:48:37.74 delay 2 seconds @18:48:39.75 PSV.dial :PML @18:48:39.76 delay 2 seconds @18:48:41.78 PRV.dial :air @18:48:42.52 delay 2 seconds @18:48:44.58 PS.seek 1.499ml @18:49:00.69 delay 5 seconds @18:49:05.70 PS.seek 2.500ml @18:49:16.72 PSV.dial :air @18:49:17.18 PS.seek 0.000ml @18:49:43.44 mixing lysate with speDil @18:49:43.45 PSV.dial :PML @18:49:43.91 CBV.dial :PRV @18:49:43.92 PRV.dial :air @18:49:43.93 delay 2 seconds @18:49:45.94 PRV.dial :speDil @18:49:46.41 delay 2 seconds @18:49:48.47 PS.reconfigure PSslow1 @18:49:48.52 PS.seek 0.225ml @18:50:12.03 delay 5 seconds @18:50:17.04 PRV.dial CBV @18:50:17.80 delay 2 seconds @18:50:19.86 PS.seek 0.475ml @18:50:45.87 delay 5 seconds @18:50:50.88 PRV.dial :air @18:50:51.33 delay 2 seconds @18:50:53.34 PS.seek 1.000ml @18:51:47.50 delay 10 seconds @18:51:57.51 PRV.dial CBV @18:51:57.96 CTV.dial :air,avoiding => intake @18:51:57.97 PSV.dial :air @18:51:58.44 PS.reconfigure PSconfig @18:51:58.48 PS.seek 2.500ml @18:52:14.59 delay 3 seconds @18:52:17.60 PSV.dial PTV @18:52:18.34 PTV.dial :PRV,avoiding => puck @18:52:19.08 PS.seek 0.000ml @18:52:45.37 PSV.dial :air @18:52:46.11 PS.seek 2.500ml @18:53:12.37 delay 3 seconds @18:53:15.38 PSV.dial PTV @18:53:16.12 PTV.dial :PRV,avoiding => puck @18:53:16.14 PS.seek 0.000ml @18:53:26.83 Session Ended @18:53:42.40
Pushing modified lysate into AIV Injection Loop @18:53:42.41 CBV.dial AIV @18:53:42.42 AIV.dial :bypass @18:53:42.43 PRV.dial CBV @18:53:42.44 PSV.dial :air @18:53:43.17 PS.seek 2.500ml @18:54:09.36 delay 3 seconds @18:54:12.38 PSV.dial :PML @18:54:12.82 PTV.dial :PRVmixing,avoiding => puck @18:54:13.59 PS.reconfigure PSslow1 @18:54:13.62 PS.seek 1.740ml @18:55:31.27 delay 5 seconds @18:55:36.28 AIV.dial :sample @18:55:36.83 AWV.select :waste @18:55:36.99 PS.reconfigure PSslow2 @18:55:37.02 PS.seek 1.220ml @18:57:23.01 delay 15 seconds @18:57:38.02 AIV.dial :bypass @18:57:38.59 PS.reconfigure PSconfig @18:57:38.68 PS.seek 0.000ml @18:57:51.95 PSV.dial :air @18:57:52.41 PS.seek 2.500ml @18:58:18.60 PSV.dial :PML @18:58:19.05 PS.seek 0.000ml @18:58:45.29 AIV.dial :coilToMFB @18:58:45.30 AWV.select :air @18:58:45.44 Extracting Nucleic Acid from AIV @18:58:45.46 SPE.hold 2:46:40,55.0C @18:58:45.48
Beginning samplePassD @18:58:45.50 ASV.dial :SPEdelivery @18:58:45.61
AWV.select :air @18:58:45.62 AIV.dial :bypass @18:58:45.63 PSV.dial PSR @18:58:46.06 delay 20 seconds @18:58:46.70
PSR.select :killMFB @18:58:46.81 PS.seek 1.500ml @18:59:02.95 delay 5 seconds @18:59:06.07 primeLysateINT @18:59:06.08 AWV.select :air @18:59:06.09 AIV.dial :coilToMFB @18:59:06.10 ATV.dial :AIV @18:59:07.45 AS.reconfigure ASslow1 @18:59:07.48 AS.seek 0.241ml @18:59:07.96
PSV.dial :waste1 @18:59:08.41 PS.seek 0.000ml @18:59:24.57 PSV.dial PSR @18:59:25.02 PSR.select :air @18:59:25.12 PS.seek 2.500ml @18:59:51.35 PRV.dial CBV @18:59:51.37
CBV.dial PRV @18:59:51.85 PSV.dial :mixloop @18:59:52.90 CTV.dial :air,avoiding => intake @18:59:52.91 PTV.dial :PRVmixing @18:59:52.93 PS.seek 0.000ml @18:59:57.04 delay 10 seconds @19:00:07.05 ATV.dial :air2 @19:00:07.64 AS.seek 0.271ml @19:00:14.02 ATV.dial :waste2 @19:00:14.38 AS.reconfigure ASnorm @19:00:14.41 AS.seek empty @19:00:19.19
PSV.dial PSR @19:00:20.24 PSR.select :flush @19:00:20.34 PS.seek 1.500ml @19:00:28.68 Lysate primed! @19:00:28.69 AFV.select :holdingCoil @19:00:28.70 ATV.dial :air2 @19:00:29.09 AS.reconfigure ASslow1 @19:00:29.12 AS.seek 0.069ml @19:00:36.38
delay 5 seconds @19:00:41.39 PSV.dial :waste1 @19:00:41.84 PS.seek 0.000ml @19:00:43.66 delay 3 seconds @19:00:46.67 ATV.dial :AIV @19:00:47.34 AS.seek 0.469ml @19:00:57.94
PSV.dial PSR @19:00:58.39 PSR.select :air @19:00:58.49 PS.seek 2.500ml @19:01:24.69 PRV.dial CBV @19:01:24.71
CBV.dial PRV @19:01:24.72 PSV.dial :mixloop @19:01:25.74 CTV.dial :air,avoiding => intake @19:01:25.75 PTV.dial :PRVmixing @19:01:25.77 PS.seek 0.000ml @19:01:52.00 PSV.dial PSR @19:01:53.14 PSR.select :flush @19:01:53.24 PS.seek 1.500ml @19:02:09.18 delay 5 seconds @19:02:09.31
delay 5 seconds @19:02:14.23 Solid Phase Extraction Heater at 54.0C @19:02:14.24 ATV.dial ASV @19:02:14.32
PSV.dial :waste1 @19:02:14.58 ASV.dial :SPEdelivery @19:02:14.64 AS.seek 0.041ml @19:02:14.78
PS.seek 0.000ml @19:02:31.03 PSV.dial PSR @19:02:31.59 PSR.select :air @19:02:31.69 PS.seek 2.500ml @19:02:57.91 PRV.dial CBV @19:02:57.93
CBV.dial PRV @19:02:57.94 PSV.dial :mixloop @19:02:58.97 CTV.dial :air,avoiding => intake @19:02:58.98 PTV.dial :PRVmixing @19:02:59.00 PS.seek 0.000ml @19:03:25.34 PSV.dial PSR @19:03:26.36 PSR.select :flush @19:03:26.46 PS.seek 1.500ml @19:03:42.17 delay 10 seconds @19:03:42.60
delay 5 seconds @19:03:47.61 PSV.dial :waste1 @19:03:48.06 PS.seek 0.000ml @19:03:52.18 rinseLysateLineINT @19:03:52.19 AFV.select :carrierSolution @19:03:52.28 delay 2 seconds @19:03:54.29 AS.reconfigure ASconfig @19:03:54.32 AS.seek 1.000ml @19:04:04.16
PSV.dial PSR @19:04:04.62 PSR.select :air @19:04:04.72 PS.seek 2.500ml @19:04:30.97 PRV.dial CBV @19:04:30.99
CBV.dial PRV @19:04:31.00 PSV.dial :mixloop @19:04:32.01 CTV.dial :air,avoiding => intake @19:04:32.02 PTV.dial :PRVmixing @19:04:32.03 PS.reconfigure PSslow @19:04:32.07 PS.seek 1.200ml @19:04:50.51 delay 1 second @19:04:51.52 AFV.select :holdingCoil @19:04:51.61 delay 1 second @19:04:52.62 ATV.dial :AIV @19:04:52.96 AIV.dial :coilToMFB @19:04:52.97 AWV.select :waste @19:04:53.06 AS.reconfigure ASslow0 @19:04:53.10 AS.seek 0.000ml @19:04:59.07
PSV.dial :waste1 @19:05:00.37 PS.reconfigure PSconfig @19:05:00.40 PS.seek 0.000ml @19:05:13.47 PSV.dial :air @19:05:14.50 PS.seek 2.500ml @19:05:40.81 PSV.dial :mixloop @19:05:41.26 PS.reconfigure PSslow @19:05:41.29 PS.seek 0.000ml @19:06:32.66 PSV.dial :air @19:06:33.12 PS.reconfigure PSconfig @19:06:33.15 PS.seek 2.500ml @19:06:35.43 ATV.dial :air2 @19:06:36.09 AS.reconfigure ASslow1 @19:06:36.12 AS.seek 0.050ml @19:06:46.61 ATV.dial :AIV @19:06:47.18 AS.seek 0.010ml @19:06:55.60 ATV.dial :waste @19:06:56.87 AS.seek empty @19:06:59.28 ethanol wash @19:06:59.29 ATV.dial :air2 @19:06:59.34
PSV.dial :mixloop @19:06:59.81 PS.reconfigure PSslow @19:06:59.84 PS.seek 0.000ml @19:07:00.32 AS.reconfigure ASslow2 @19:07:00.35 AS.seek 0.009ml @19:07:10.82 delay 3 seconds @19:07:13.83 Pulling 0.4ml of Ethanol Wash @19:07:13.84 ATV.dial :ethanol @19:07:14.88 AS.reconfigure ASslow1 @19:07:14.91 AS.seek 0.409ml @19:07:51.22
Cleaning Collection Syringe @19:07:51.23 CSV.dial CSR @19:07:51.66 CSR.select :flush @19:07:51.76 CS.seek 4.000ml @19:08:02.66 delay 5 seconds @19:08:07.67 CSR.select :air @19:08:07.76 CS.seek 10.000ml @19:08:23.75 CSV.dial CTV @19:08:23.77
CTV.dial CSV @19:08:25.11 CBV.dial :waste1 @19:08:25.59 CS.seek 0.000ml @19:08:36.75 delay 3 seconds @19:08:39.76 ATV.dial :air1 @19:08:40.09 delay 3 seconds @19:08:43.15 AS.seek 0.434ml @19:08:48.52 delay 3 seconds @19:08:51.53 ATV.dial :ethanol @19:08:51.82
CSV.dial CSR @19:08:51.92 AS.seek 0.419ml @19:08:52.33
CSR.select :flush @19:08:52.43 CS.seek 4.000ml @19:08:55.23 delay 3 seconds @19:08:58.24 ATV.dial ASV @19:08:59.36 AS.seek 0.092ml @19:09:03.44
delay 5 seconds @19:09:08.45 CSR.select :air @19:09:08.54 CS.seek 10.000ml @19:09:24.47 CSV.dial CTV @19:09:24.49
CTV.dial CSV @19:09:25.01 CBV.dial :waste1 @19:09:25.02 CS.seek 0.000ml @19:09:51.11 Garaging FlushPuck in Collection Clamp @19:09:51.16 Elbow.reconfigure TightElbow @19:09:51.20 Elbow.seek CC @19:09:51.30 Elbow.reconfigure LooseElbow @19:09:51.38 Forearm.seek CC @19:09:52.32 CC.seek sealed @19:09:53.22 Hand.grip @19:09:53.90 CC.seek open @19:09:57.21 Forearm.seek retract @19:09:58.17 Elbow.reconfigure TightElbow @19:09:58.21 Elbow.seek garage @19:10:00.95 Elbow.reconfigure LooseElbow @19:10:01.03 Forearm.reconfigure TightForearm @19:10:01.06 Forearm.seek garage @19:10:02.25 Hand.release @19:10:03.06 Forearm.reconfigure LooseForearm @19:10:03.09 Forearm.seek retract @19:10:04.13 Whole Cell Archive sampling 1000ml @19:10:04.14 Loading wcr puck from tube 2 into Collection Clamp @19:10:04.32 SC.reconfigure SCtight @19:10:04.35 SC.seek 2 @19:10:06.28 delay 3 seconds @19:10:06.59 SC.reconfigure SCloose @19:10:06.80 Elbow.reconfigure TightElbow @19:10:06.91 Elbow.seek 2 @19:10:08.77 Forearm.reconfigure TightForearm @19:10:08.80 Forearm.seek 2 + 800 counts @19:10:09.29 ATV.dial :waste2 @19:10:09.89 SE.reconfigure SEpick @19:10:09.95 AS.reconfigure ASconfig @19:10:09.98 SE.seek beamBreak @19:10:10.01 AS.seek empty @19:10:11.12 SE.configure SEconfig @19:10:11.25 Elbow.seek 2 @19:10:11.39 Forearm.seek 2 + 300 counts @19:10:11.65 Forearm.reconfigure LooseForearm @19:10:11.69 Hand.grip @19:10:12.41 SE.seek down @19:10:12.56 Forearm.seek retract @19:10:13.30 SE.coast @19:10:13.52 Elbow.seek CC @19:10:15.05 Elbow.reconfigure LooseElbow @19:10:15.13 Forearm.reconfigure TightForearm @19:10:15.16 Forearm.seek CC @19:10:15.73 ATV.dial :air2 @19:10:16.08 AS.reconfigure ASslow1 @19:10:16.11 AS.seek 1.000ml @19:10:16.77 CC.seek closed @19:10:20.55 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.97 @19:10:20.56 Hand.release @19:10:21.31 Forearm.reconfigure LooseForearm @19:10:21.35 Forearm.seek retract @19:10:22.31 SC.coast @19:10:22.35 collectSample 1000ml w/bubble point of 55psi @19:10:22.36 Priming 7ml Intake.select :close @19:10:22.37 Exhaust.select :close @19:10:22.38 SS.hold @19:10:22.54 Calibrating pressure sensors @19:10:22.55 CTV.dial :air @19:10:23.82 SSV.dial :spit,avoiding => exhaust @19:10:23.83 SS.reconfigure SSfast @19:10:23.86 SS.seek 5.00ml @19:10:26.54 SSV.dial CBV @19:10:26.56 CBV.dial SSV @19:10:27.91 delay 3.8 seconds @19:10:31.80 SSV.dial :spit @19:10:33.11 SS.seek 0.00ml @19:10:35.83 SSV.dial CBV @19:10:37.13 CTV.dial :intake @19:10:37.87 SSV.dial CBV @19:10:37.88 CBV.dial SSV @19:10:37.89 Allowing 55s for 7.0ml of remaining 7.0ml @19:10:37.90 Intake.select :open @19:10:40.03 Intake @33.6psia, vacuum from 20.0 to 24.0psi @19:10:40.05 SS.reconfigure forSampling @19:10:40.08 SS.seek 7.00ml @19:10:43.79 Vacuum too high -- waiting up to 40s @19:10:45.14 Intake @31.1psia, vacuum from 20.0 to 24.0psi @19:10:45.16 SS.reconfigure forSampling @19:10:45.19 SS.seek 7.00ml @19:10:47.71 Stroke completed -- waiting for pressure to equalize ... @19:10:52.49 Intake.select :close @19:10:52.62 SSV.dial :exhaust @19:10:53.38 Exhaust.select :open @19:10:53.48 SS.reconfigure SSexhale @19:10:53.52 SS.seek 0.00ml @19:10:56.99 Exhaust.select :close @19:10:57.09 Sampling 1000.0ml @19:10:57.10 Intake.select :close @19:10:57.11 Exhaust.select :close @19:10:57.12 SS.reconfigure SSconfig @19:10:57.15 SS.hold @19:10:57.30 CTV.dial :intake @19:10:57.31 SSV.dial CBV @19:10:57.33 CBV.dial SSV @19:10:58.11 Allowing 185s for 25.0ml of remaining 1000.0ml @19:10:58.12 Intake.select :open @19:11:00.25 Intake @33.5psia, vacuum from 20.0 to 24.0psi @19:11:00.27 SS.reconfigure forSampling @19:11:00.30 SS.seek 25.00ml @19:11:02.98 Vacuum too high -- waiting up to 40s @19:11:03.47 Intake @31.0psia, vacuum from 20.0 to 24.0psi @19:11:03.48 SS.reconfigure forSampling @19:11:03.52 SS.seek 25.00ml @19:11:06.04 Vacuum too high -- waiting up to 40s @19:11:06.74 Intake @31.3psia, vacuum from 20.0 to 24.0psi @19:11:06.75 SS.reconfigure forSampling @19:11:06.79 SS.seek 25.00ml @19:11:09.51 Vacuum too high -- waiting up to 40s @19:11:10.00 Intake @30.3psia, vacuum from 20.0 to 24.0psi @19:11:10.02 SS.reconfigure forSampling @19:11:10.05 SS.seek 25.00ml @19:11:18.70 Vacuum too high -- waiting up to 40s @19:11:19.18 Intake @30.1psia, vacuum from 20.0 to 24.0psi @19:11:19.19 SS.reconfigure forSampling @19:11:19.22 SS.seek 25.00ml @19:11:27.16 Stroke completed -- waiting for pressure to equalize ... @19:11:27.80 Intake.select :close @19:11:27.94 SSV.dial :exhaust @19:11:28.69 Exhaust.select :open @19:11:28.79 SS.reconfigure SSexhale @19:11:28.83 SS.seek 0.00ml @19:11:37.66 Exhaust.select :close @19:11:37.76 SSV.dial CBV @19:11:37.78 CBV.dial SSV @19:11:38.55 Allowing 185s for 25.0ml of remaining 975.0ml @19:11:38.57 Intake.select :open @19:11:40.70 Intake @33.5psia, vacuum from 20.0 to 24.0psi @19:11:40.72 SS.reconfigure forSampling @19:11:40.75 SS.seek 25.00ml @19:12:05.44 Stroke completed -- waiting for pressure to equalize ... @19:12:06.08 Intake.select :close @19:12:06.22 SSV.dial :exhaust @19:12:06.98 Exhaust.select :open @19:12:07.08 SS.reconfigure SSexhale @19:12:07.12 SS.seek 0.00ml @19:12:15.97 Exhaust.select :close @19:12:16.07 SSV.dial CBV @19:12:16.09 CBV.dial SSV @19:12:16.84 Allowing 185s for 25.0ml of remaining 950.0ml @19:12:16.85 Intake.select :open @19:12:18.99 Intake @33.7psia, vacuum from 20.0 to 24.0psi @19:12:19.01 SS.reconfigure forSampling @19:12:19.04 SS.seek 25.00ml @19:12:44.23 Stroke completed -- waiting for pressure to equalize ... @19:12:44.87 Intake.select :close @19:12:45.02 SSV.dial :exhaust @19:12:45.78 Exhaust.select :open @19:12:45.88 SS.reconfigure SSexhale @19:12:45.92 SS.seek 0.00ml @19:12:54.61 Exhaust.select :close @19:12:54.71 SSV.dial CBV @19:12:54.73 CBV.dial SSV @19:12:55.50 Allowing 185s for 25.0ml of remaining 925.0ml @19:12:55.51 Intake.select :open @19:12:57.65 Intake @33.9psia, vacuum from 20.0 to 24.0psi @19:12:57.67 SS.reconfigure forSampling @19:12:57.70 SS.seek 25.00ml @19:13:23.15 Stroke completed -- waiting for pressure to equalize ... @19:13:23.79 Intake.select :close @19:13:23.93 SSV.dial :exhaust @19:13:24.68 Exhaust.select :open @19:13:24.78 SS.reconfigure SSexhale @19:13:24.82 SS.seek 0.00ml @19:13:33.31 Exhaust.select :close @19:13:33.42 SSV.dial CBV @19:13:33.44 CBV.dial SSV @19:13:34.21 Allowing 185s for 25.0ml of remaining 900.0ml @19:13:34.22 Intake.select :open @19:13:36.36 Intake @33.8psia, vacuum from 20.0 to 24.0psi @19:13:36.38 SS.reconfigure forSampling @19:13:36.41 SS.seek 25.00ml @19:13:41.10 delay 5 seconds @19:13:46.11 ATV.dial ASV @19:13:46.83 ASV.dial :SPEdelivery @19:13:46.85 AS.seek 0.020ml @19:14:01.65 Stroke completed -- waiting for pressure to equalize ... @19:14:02.29 Intake.select :close @19:14:02.43 SSV.dial :exhaust @19:14:03.18 Exhaust.select :open @19:14:03.28 SS.reconfigure SSexhale @19:14:03.32 SS.seek 0.00ml @19:14:11.90 Exhaust.select :close @19:14:12.01 SSV.dial CBV @19:14:12.03 CBV.dial SSV @19:14:12.79 Allowing 185s for 25.0ml of remaining 875.0ml @19:14:12.80 Intake.select :open @19:14:14.94 Intake @33.7psia, vacuum from 20.0 to 24.0psi @19:14:14.96 SS.reconfigure forSampling @19:14:14.99 SS.seek 25.00ml @19:14:39.70 Stroke completed -- waiting for pressure to equalize ... @19:14:40.34 Intake.select :close @19:14:40.47 SSV.dial :exhaust @19:14:41.23 Exhaust.select :open @19:14:41.34 SS.reconfigure SSexhale @19:14:41.38 SS.seek 0.00ml @19:14:49.85 Exhaust.select :close @19:14:49.96 SSV.dial CBV @19:14:49.98 CBV.dial SSV @19:14:50.74 Allowing 185s for 25.0ml of remaining 850.0ml @19:14:50.75 Intake.select :open @19:14:52.89 Intake @33.7psia, vacuum from 20.0 to 24.0psi @19:14:52.91 SS.reconfigure forSampling @19:14:52.94 SS.seek 25.00ml @19:15:17.57 Stroke completed -- waiting for pressure to equalize ... @19:15:18.21 Intake.select :close @19:15:18.34 SSV.dial :exhaust @19:15:19.10 Exhaust.select :open @19:15:19.21 SS.reconfigure SSexhale @19:15:19.25 SS.seek 0.00ml @19:15:27.83 Exhaust.select :close @19:15:27.94 SSV.dial CBV @19:15:27.96 CBV.dial SSV @19:15:28.72 Allowing 185s for 25.0ml of remaining 825.0ml @19:15:28.73 Intake.select :open @19:15:30.88 Intake @33.9psia, vacuum from 20.0 to 24.0psi @19:15:30.90 SS.reconfigure forSampling @19:15:30.93 SS.seek 25.00ml @19:15:55.66 Stroke completed -- waiting for pressure to equalize ... @19:15:56.81 Intake.select :close @19:15:56.96 SSV.dial :exhaust @19:15:57.72 Exhaust.select :open @19:15:57.82 SS.reconfigure SSexhale @19:15:57.86 SS.seek 0.00ml @19:16:06.28 Exhaust.select :close @19:16:06.38 SSV.dial CBV @19:16:06.40 CBV.dial SSV @19:16:07.16 Allowing 185s for 25.0ml of remaining 800.0ml @19:16:07.17 Intake.select :open @19:16:09.31 Intake @33.9psia, vacuum from 20.0 to 24.0psi @19:16:09.33 SS.reconfigure forSampling @19:16:09.36 SS.seek 25.00ml @19:16:34.02 Stroke completed -- waiting for pressure to equalize ... @19:16:34.66 Intake.select :close @19:16:34.80 SSV.dial :exhaust @19:16:35.56 Exhaust.select :open @19:16:35.66 SS.reconfigure SSexhale @19:16:35.70 SS.seek 0.00ml @19:16:44.25 Exhaust.select :close @19:16:44.35 SSV.dial CBV @19:16:44.37 CBV.dial SSV @19:16:45.13 Allowing 185s for 25.0ml of remaining 775.0ml @19:16:45.14 Intake.select :open @19:16:47.28 Intake @33.9psia, vacuum from 20.0 to 24.0psi @19:16:47.30 SS.reconfigure forSampling @19:16:47.33 SS.seek 25.00ml @19:17:07.01 delay 5 seconds @19:17:12.02 ATV.dial :air2 @19:17:12.66 Stroke completed -- waiting for pressure to equalize ... @19:17:12.88 AS.seek 0.520ml @19:17:13.30 Intake.select :close @19:17:13.44 SSV.dial :exhaust @19:17:14.20 Exhaust.select :open @19:17:14.30 SS.reconfigure SSexhale @19:17:14.34 SS.seek 0.00ml @19:17:22.92 Exhaust.select :close @19:17:23.03 SSV.dial CBV @19:17:23.05 CBV.dial SSV @19:17:23.81 Allowing 185s for 25.0ml of remaining 750.0ml @19:17:23.82 Intake.select :open @19:17:25.96 Intake @33.9psia, vacuum from 20.0 to 24.0psi @19:17:25.98 SS.reconfigure forSampling @19:17:26.01 SS.seek 25.00ml @19:17:50.75 Stroke completed -- waiting for pressure to equalize ... @19:17:51.39 Intake.select :close @19:17:51.53 SSV.dial :exhaust @19:17:52.29 Exhaust.select :open @19:17:52.40 SS.reconfigure SSexhale @19:17:52.43 SS.seek 0.00ml @19:18:01.04 Exhaust.select :close @19:18:01.15 SSV.dial CBV @19:18:01.17 CBV.dial SSV @19:18:01.92 Allowing 185s for 25.0ml of remaining 725.0ml @19:18:01.93 Intake.select :open @19:18:04.07 Intake @33.9psia, vacuum from 20.0 to 24.0psi @19:18:04.09 SS.reconfigure forSampling @19:18:04.12 SS.seek 25.00ml @19:18:28.79 Stroke completed -- waiting for pressure to equalize ... @19:18:29.43 Intake.select :close @19:18:29.58 SSV.dial :exhaust @19:18:30.34 Exhaust.select :open @19:18:30.45 SS.reconfigure SSexhale @19:18:30.49 SS.seek 0.00ml @19:18:39.18 Exhaust.select :close @19:18:39.29 SSV.dial CBV @19:18:39.31 CBV.dial SSV @19:18:40.07 Allowing 185s for 25.0ml of remaining 700.0ml @19:18:40.08 Intake.select :open @19:18:42.22 Intake @34.0psia, vacuum from 20.0 to 24.0psi @19:18:42.24 SS.reconfigure forSampling @19:18:42.27 SS.seek 25.00ml @19:18:55.12 delay 5 seconds @19:19:00.13 ATV.dial ASV @19:19:00.84 ASV.dial :wasteR @19:19:01.44 AS.seek 0.300ml @19:19:07.60 Stroke completed -- waiting for pressure to equalize ... @19:19:08.24 Intake.select :close @19:19:08.37 SSV.dial :exhaust @19:19:09.13 Exhaust.select :open @19:19:09.24 SS.reconfigure SSexhale @19:19:09.28 SS.seek 0.00ml @19:19:17.84 Exhaust.select :close @19:19:17.95 SSV.dial CBV @19:19:17.97 CBV.dial SSV @19:19:18.73 Allowing 185s for 25.0ml of remaining 675.0ml @19:19:18.74 Intake.select :open @19:19:20.88 Intake @34.0psia, vacuum from 20.0 to 24.0psi @19:19:20.90 SS.reconfigure forSampling @19:19:20.93 SS.seek 25.00ml @19:19:45.60 Stroke completed -- waiting for pressure to equalize ... @19:19:46.24 Intake.select :close @19:19:46.39 SSV.dial :exhaust @19:19:46.56 delay 20 seconds @19:19:47.15 Exhaust.select :open @19:19:47.25 SS.reconfigure SSexhale @19:19:47.29 SS.seek 0.00ml @19:19:55.74 Exhaust.select :close @19:19:55.85 SSV.dial CBV @19:19:55.87 CBV.dial SSV @19:19:56.63 Allowing 185s for 25.0ml of remaining 650.0ml @19:19:56.64 Intake.select :open @19:19:59.31 Intake @34.0psia, vacuum from 20.0 to 24.0psi @19:19:59.32 SS.reconfigure forSampling @19:19:59.35 SS.seek 25.00ml @19:20:06.57 AS.seek 0.070ml @19:20:24.85 Stroke completed -- waiting for pressure to equalize ... @19:20:25.49 Intake.select :close @19:20:25.64 SSV.dial :exhaust @19:20:26.40 Exhaust.select :open @19:20:26.50 SS.reconfigure SSexhale @19:20:26.54 SS.seek 0.00ml @19:20:35.20 Exhaust.select :close @19:20:35.31 SSV.dial CBV @19:20:35.33 CBV.dial SSV @19:20:36.09 Allowing 185s for 25.0ml of remaining 625.0ml @19:20:36.10 Intake.select :open @19:20:38.24 Intake @34.2psia, vacuum from 20.0 to 24.0psi @19:20:38.26 SS.reconfigure forSampling @19:20:38.29 SS.seek 25.00ml @19:20:53.73 delay 20 seconds @19:21:03.00 Stroke completed -- waiting for pressure to equalize ... @19:21:03.64 Intake.select :close @19:21:03.78 SSV.dial :exhaust @19:21:04.54 Exhaust.select :open @19:21:04.64 SS.reconfigure SSexhale @19:21:04.68 SS.seek 0.00ml @19:21:13.18 Exhaust.select :close @19:21:13.28 SSV.dial CBV @19:21:13.30 CBV.dial SSV @19:21:13.74 ATV.dial :waste2 @19:21:14.06 Allowing 185s for 25.0ml of remaining 600.0ml @19:21:14.07 Intake.select :open @19:21:14.37 AS.reconfigure ASconfig @19:21:14.40 AS.seek empty @19:21:16.21 Intake @34.1psia, vacuum from 20.0 to 24.0psi @19:21:16.23 SS.reconfigure forSampling @19:21:16.26 SS.seek 25.00ml @19:21:18.78 Elution @19:21:18.79 ATV.dial :air1 @19:21:19.83 AS.reconfigure ASslow1 @19:21:19.86 AS.seek 0.049ml @19:21:30.36 delay 3 seconds @19:21:33.37 ATV.dial :ElutionWater @19:21:33.94 AS.seek 0.109ml @19:21:40.89 Stroke completed -- waiting for pressure to equalize ... @19:21:42.11 Intake.select :close @19:21:42.24 SSV.dial :exhaust @19:21:42.99 Exhaust.select :open @19:21:43.09 SS.reconfigure SSexhale @19:21:43.13 SS.seek 0.00ml @19:21:46.44 delay 3 seconds @19:21:49.45 ATV.dial ASV,via => air2 @19:21:51.08 ASV.dial :SPEelution @19:21:51.70 Exhaust.select :close @19:21:51.80 SSV.dial CBV @19:21:51.82 CBV.dial SSV @19:21:51.87 AFV.select :carrierSolution @19:21:52.01 AS.reconfigure ASnorm @19:21:52.04 AS.seek 0.759ml @19:21:52.58 Allowing 185s for 25.0ml of remaining 575.0ml @19:21:52.59 Intake.select :open @19:21:54.72 Intake @34.2psia, vacuum from 20.0 to 24.0psi @19:21:54.74 SS.reconfigure forSampling @19:21:54.77 SS.seek 25.00ml @19:22:19.43 Stroke completed -- waiting for pressure to equalize ... @19:22:20.65 Intake.select :close @19:22:20.80 SSV.dial :exhaust @19:22:21.56 Exhaust.select :open @19:22:21.67 SS.reconfigure SSexhale @19:22:21.71 SS.seek 0.00ml @19:22:25.53 delay 3 seconds @19:22:28.54 AFV.select :holdingCoil @19:22:28.68 AS.reconfigure ASslow1 @19:22:28.71 AS.seek 0.489ml @19:22:30.29 Exhaust.select :close @19:22:30.39 SSV.dial CBV @19:22:30.41 CBV.dial SSV @19:22:31.19 Allowing 185s for 25.0ml of remaining 550.0ml @19:22:31.20 Intake.select :open @19:22:33.33 Intake @34.1psia, vacuum from 20.0 to 24.0psi @19:22:33.35 SS.reconfigure forSampling @19:22:33.38 SS.seek 25.00ml @19:22:58.87 Stroke completed -- waiting for pressure to equalize ... @19:22:59.51 Intake.select :close @19:22:59.64 SSV.dial :exhaust @19:23:00.40 Exhaust.select :open @19:23:00.50 SS.reconfigure SSexhale @19:23:00.54 SS.seek 0.00ml @19:23:09.02 Exhaust.select :close @19:23:09.13 SSV.dial CBV @19:23:09.15 CBV.dial SSV @19:23:09.91 Allowing 185s for 25.0ml of remaining 525.0ml @19:23:09.92 Intake.select :open @19:23:12.06 Intake @34.1psia, vacuum from 20.0 to 24.0psi @19:23:12.08 SS.reconfigure forSampling @19:23:12.11 SS.seek 25.00ml @19:23:24.06 delay 5 seconds @19:23:29.12 AS.reconfigure ASslow2 @19:23:29.15 AS.seek 0.454ml @19:23:36.79 Stroke completed -- waiting for pressure to equalize ... @19:23:38.02 Intake.select :close @19:23:38.16 SSV.dial :exhaust @19:23:38.91 Exhaust.select :open @19:23:39.53 SS.reconfigure SSexhale @19:23:39.57 SS.seek 0.00ml @19:23:48.39 Exhaust.select :close @19:23:48.49 SSV.dial CBV @19:23:48.51 CBV.dial SSV @19:23:49.28 Allowing 185s for 25.0ml of remaining 500.0ml @19:23:49.30 Intake.select :open @19:23:51.43 Intake @34.2psia, vacuum from 20.0 to 24.0psi @19:23:51.45 SS.reconfigure forSampling @19:23:51.48 SS.seek 25.00ml @19:24:05.06 delay 1:00 @19:24:16.05 Stroke completed -- waiting for pressure to equalize ... @19:24:17.80 Intake.select :close @19:24:17.94 SSV.dial :exhaust @19:24:18.69 Exhaust.select :open @19:24:18.79 SS.reconfigure SSexhale @19:24:18.83 SS.seek 0.00ml @19:24:27.33 Exhaust.select :close @19:24:27.43 SSV.dial CBV @19:24:27.45 CBV.dial SSV @19:24:28.22 Allowing 185s for 25.0ml of remaining 475.0ml @19:24:28.24 Intake.select :open @19:24:30.37 Intake @34.2psia, vacuum from 20.0 to 24.0psi @19:24:30.39 SS.reconfigure forSampling @19:24:30.42 SS.seek 25.00ml @19:24:55.17 Stroke completed -- waiting for pressure to equalize ... @19:24:55.81 Intake.select :close @19:24:55.95 SSV.dial :exhaust @19:24:56.70 Exhaust.select :open @19:24:56.81 SS.reconfigure SSexhale @19:24:56.85 SS.seek 0.00ml @19:25:05.07 pushing eluate to holding coil @19:25:05.13 AS.seek 0.294ml @19:25:05.70 Exhaust.select :close @19:25:05.80 SSV.dial CBV @19:25:05.82 CBV.dial SSV @19:25:06.60 Allowing 185s for 25.0ml of remaining 450.0ml @19:25:06.61 Intake.select :open @19:25:08.74 Intake @34.3psia, vacuum from 20.0 to 24.0psi @19:25:08.76 SS.reconfigure forSampling @19:25:08.79 SS.seek 25.00ml @19:25:33.36 Stroke completed -- waiting for pressure to equalize ... @19:25:34.59 Intake.select :close @19:25:34.73 SSV.dial :exhaust @19:25:35.48 Exhaust.select :open @19:25:35.59 SS.reconfigure SSexhale @19:25:35.63 SS.seek 0.00ml @19:25:44.28 Exhaust.select :close @19:25:44.39 SSV.dial CBV @19:25:44.41 CBV.dial SSV @19:25:45.17 Allowing 185s for 25.0ml of remaining 425.0ml @19:25:45.18 Intake.select :open @19:25:47.32 Intake @34.3psia, vacuum from 20.0 to 24.0psi @19:25:47.34 SS.reconfigure forSampling @19:25:47.37 SS.seek 25.00ml @19:26:12.12 Stroke completed -- waiting for pressure to equalize ... @19:26:13.35 Intake.select :close @19:26:13.48 SSV.dial :exhaust @19:26:14.23 Exhaust.select :open @19:26:14.33 SS.reconfigure SSexhale @19:26:14.37 SS.seek 0.00ml @19:26:22.90 Exhaust.select :close @19:26:23.01 SSV.dial CBV @19:26:23.03 CBV.dial SSV @19:26:23.79 Allowing 185s for 25.0ml of remaining 400.0ml @19:26:23.80 Intake.select :open @19:26:25.94 Intake @34.3psia, vacuum from 20.0 to 24.0psi @19:26:25.96 SS.reconfigure forSampling @19:26:25.99 SS.seek 25.00ml @19:26:50.60 Stroke completed -- waiting for pressure to equalize ... @19:26:51.82 Intake.select :close @19:26:51.95 SSV.dial :exhaust @19:26:52.71 Exhaust.select :open @19:26:52.82 SS.reconfigure SSexhale @19:26:52.86 SS.seek 0.00ml @19:27:01.43 Exhaust.select :close @19:27:01.54 SSV.dial CBV @19:27:01.56 CBV.dial SSV @19:27:02.32 Allowing 185s for 25.0ml of remaining 375.0ml @19:27:02.33 Intake.select :open @19:27:04.47 Intake @34.3psia, vacuum from 20.0 to 24.0psi @19:27:04.49 SS.reconfigure forSampling @19:27:04.52 SS.seek 25.00ml @19:27:29.21 Stroke completed -- waiting for pressure to equalize ... @19:27:30.44 Intake.select :close @19:27:30.57 SSV.dial :exhaust @19:27:31.32 Exhaust.select :open @19:27:31.43 SS.reconfigure SSexhale @19:27:31.47 SS.seek 0.00ml @19:27:40.10 Exhaust.select :close @19:27:40.21 SSV.dial CBV @19:27:40.23 CBV.dial SSV @19:27:40.99 Allowing 185s for 25.0ml of remaining 350.0ml @19:27:41.01 Intake.select :open @19:27:43.15 Intake @34.3psia, vacuum from 20.0 to 24.0psi @19:27:43.17 SS.reconfigure forSampling @19:27:43.20 SS.seek 25.00ml @19:27:48.44 delay 30 seconds @19:28:07.88 Stroke completed -- waiting for pressure to equalize ... @19:28:09.11 Intake.select :close @19:28:09.26 SSV.dial :exhaust @19:28:10.02 Exhaust.select :open @19:28:10.12 SS.reconfigure SSexhale @19:28:10.16 SS.seek 0.00ml @19:28:18.77 Exhaust.select :close @19:28:18.81 ATV.dial :waste,via => air3 @19:28:18.87 SSV.dial CBV @19:28:18.89 CBV.dial SSV @19:28:19.65 Allowing 185s for 25.0ml of remaining 325.0ml @19:28:19.66 Intake.select :open @19:28:20.24 AS.reconfigure ASconfig @19:28:20.27 AS.seek empty @19:28:21.80 Intake @34.2psia, vacuum from 20.0 to 24.0psi @19:28:21.82 SS.reconfigure forSampling @19:28:21.85 SS.seek 25.00ml @19:28:37.73 Parking Bleach in LysateSource line and SPEcolumn @19:28:37.74 ATV.dial :air3 @19:28:38.32 AS.reconfigure ASslow1 @19:28:38.35 AS.seek 0.029ml @19:28:44.81 ATV.dial :ElutionWater @19:28:45.30 AS.seek 0.059ml @19:28:46.57 Stroke completed -- waiting for pressure to equalize ... @19:28:47.80 Intake.select :close @19:28:47.94 SSV.dial :exhaust @19:28:48.69 Exhaust.select :open @19:28:48.80 SS.reconfigure SSexhale @19:28:48.84 SS.seek 0.00ml @19:28:51.67 ATV.dial :air3 @19:28:52.03 AS.seek 0.069ml @19:28:54.33 delay 2 seconds @19:28:56.34 ATV.dial :bleach @19:28:56.72 AS.seek 0.711ml @19:28:57.42 Exhaust.select :close @19:28:57.51 SSV.dial CBV @19:28:57.53 CBV.dial SSV @19:28:58.29 Allowing 185s for 25.0ml of remaining 300.0ml @19:28:58.30 Intake.select :open @19:29:00.43 Intake @34.3psia, vacuum from 20.0 to 24.0psi @19:29:00.45 SS.reconfigure forSampling @19:29:00.48 SS.seek 25.00ml @19:29:25.22 Stroke completed -- waiting for pressure to equalize ... @19:29:26.45 Intake.select :close @19:29:26.58 SSV.dial :exhaust @19:29:27.33 Exhaust.select :open @19:29:27.43 SS.reconfigure SSexhale @19:29:27.47 SS.seek 0.00ml @19:29:36.05 Exhaust.select :close @19:29:36.16 SSV.dial CBV @19:29:36.18 CBV.dial SSV @19:29:36.95 Allowing 185s for 25.0ml of remaining 275.0ml @19:29:36.96 Intake.select :open @19:29:39.10 Intake @34.4psia, vacuum from 20.0 to 24.0psi @19:29:39.12 SS.reconfigure forSampling @19:29:39.15 SS.seek 25.00ml @19:30:03.89 Stroke completed -- waiting for pressure to equalize ... @19:30:05.11 Intake.select :close @19:30:05.24 SSV.dial :exhaust @19:30:06.00 Exhaust.select :open @19:30:06.10 SS.reconfigure SSexhale @19:30:06.14 SS.seek 0.00ml @19:30:14.78 Exhaust.select :close @19:30:14.89 SSV.dial CBV @19:30:14.91 CBV.dial SSV @19:30:15.67 Allowing 185s for 25.0ml of remaining 250.0ml @19:30:15.68 Intake.select :open @19:30:17.82 Intake @34.3psia, vacuum from 20.0 to 24.0psi @19:30:17.84 SS.reconfigure forSampling @19:30:17.87 SS.seek 25.00ml @19:30:42.72 Stroke completed -- waiting for pressure to equalize ... @19:30:43.94 Intake.select :close @19:30:44.07 SSV.dial :exhaust @19:30:44.82 Exhaust.select :open @19:30:44.92 SS.reconfigure SSexhale @19:30:44.96 SS.seek 0.00ml @19:30:53.59 Exhaust.select :close @19:30:53.69 SSV.dial CBV @19:30:53.71 CBV.dial SSV @19:30:54.47 Allowing 185s for 25.0ml of remaining 225.0ml @19:30:54.48 Intake.select :open @19:30:56.62 Intake @34.5psia, vacuum from 20.0 to 24.0psi @19:30:56.64 SS.reconfigure forSampling @19:30:56.67 SS.seek 25.00ml @19:31:07.88 delay 3 seconds @19:31:10.89 ATV.dial :air3 @19:31:11.32 delay 3 seconds @19:31:14.38 AS.seek 0.736ml @19:31:19.73 delay 3 seconds @19:31:21.42 Stroke completed -- waiting for pressure to equalize ... @19:31:22.64 Intake.select :close @19:31:22.74 ATV.dial :bleach @19:31:22.79 SSV.dial :exhaust @19:31:23.18 AS.seek 0.721ml @19:31:23.57 Exhaust.select :open @19:31:24.21 SS.reconfigure SSexhale @19:31:24.25 SS.seek 0.00ml @19:31:26.49 delay 3 seconds @19:31:29.50 ATV.dial :AIV @19:31:30.55 AS.reconfigure ASconfig @19:31:30.58 AS.seek 0.015ml @19:31:32.87 Exhaust.select :close @19:31:32.97 SSV.dial CBV @19:31:32.99 CBV.dial SSV @19:31:33.76 Allowing 185s for 25.0ml of remaining 200.0ml @19:31:33.77 Intake.select :open @19:31:35.91 Intake @34.4psia, vacuum from 20.0 to 24.0psi @19:31:35.93 SS.reconfigure forSampling @19:31:35.96 SS.seek 25.00ml @19:32:00.66 Stroke completed -- waiting for pressure to equalize ... @19:32:01.89 Intake.select :close @19:32:02.04 SSV.dial :exhaust @19:32:02.79 Exhaust.select :open @19:32:02.89 SS.reconfigure SSexhale @19:32:02.93 SS.seek 0.00ml @19:32:11.49 Exhaust.select :close @19:32:11.59 SSV.dial CBV @19:32:11.61 CBV.dial SSV @19:32:12.02 ATV.dial :waste @19:32:12.38 Allowing 185s for 25.0ml of remaining 175.0ml @19:32:12.39 Intake.select :open @19:32:13.28 AS.seek empty @19:32:14.48 ATV.dial :air3 @19:32:14.56 Intake @34.3psia, vacuum from 20.0 to 24.0psi @19:32:14.57 SS.reconfigure forSampling @19:32:14.61 SS.seek 25.00ml @19:32:15.06 AS.reconfigure ASslow1 @19:32:15.10 AS.seek 0.009ml @19:32:17.43 delay 2 seconds @19:32:19.44 ATV.dial :bleach @19:32:19.81 AS.seek 0.579ml @19:32:39.37 Stroke completed -- waiting for pressure to equalize ... @19:32:40.59 Intake.select :close @19:32:40.74 SSV.dial :exhaust @19:32:41.49 Exhaust.select :open @19:32:41.60 SS.reconfigure SSexhale @19:32:41.64 SS.seek 0.00ml @19:32:50.21 Exhaust.select :close @19:32:50.32 SSV.dial CBV @19:32:50.34 CBV.dial SSV @19:32:51.13 Allowing 185s for 25.0ml of remaining 150.0ml @19:32:51.14 Intake.select :open @19:32:53.27 Intake @34.3psia, vacuum from 20.0 to 24.0psi @19:32:53.29 SS.reconfigure forSampling @19:32:53.32 SS.seek 25.00ml @19:33:18.02 Stroke completed -- waiting for pressure to equalize ... @19:33:19.25 Intake.select :close @19:33:19.39 SSV.dial :exhaust @19:33:20.14 Exhaust.select :open @19:33:20.24 SS.reconfigure SSexhale @19:33:20.28 SS.seek 0.00ml @19:33:28.89 Exhaust.select :close @19:33:29.00 SSV.dial CBV @19:33:29.02 CBV.dial SSV @19:33:29.77 Allowing 185s for 25.0ml of remaining 125.0ml @19:33:29.78 Intake.select :open @19:33:31.92 Intake @34.4psia, vacuum from 20.0 to 24.0psi @19:33:31.94 SS.reconfigure forSampling @19:33:31.97 SS.seek 25.00ml @19:33:52.54 Vacuum too high -- waiting up to 40s @19:33:52.80 Intake @31.3psia, vacuum from 20.0 to 24.0psi @19:33:52.82 SS.reconfigure forSampling @19:33:52.85 SS.seek 25.00ml @19:33:58.10 Stroke completed -- waiting for pressure to equalize ... @19:33:58.74 Intake.select :close @19:33:58.89 SSV.dial :exhaust @19:33:59.64 Exhaust.select :open @19:33:59.74 SS.reconfigure SSexhale @19:33:59.78 SS.seek 0.00ml @19:34:08.36 Exhaust.select :close @19:34:08.47 SSV.dial CBV @19:34:08.49 CBV.dial SSV @19:34:09.25 Allowing 185s for 25.0ml of remaining 100.0ml @19:34:09.26 Intake.select :open @19:34:11.40 Intake @34.3psia, vacuum from 20.0 to 24.0psi @19:34:11.42 SS.reconfigure forSampling @19:34:11.45 SS.seek 25.00ml @19:34:16.33 delay 3 seconds @19:34:19.34 ATV.dial :air3 @19:34:19.66 delay 3 seconds @19:34:22.72 AS.seek 0.604ml @19:34:28.05 delay 3 seconds @19:34:31.06 ATV.dial :bleach @19:34:31.44 AS.seek 0.589ml @19:34:34.75 delay 3 seconds @19:34:37.76 ATV.dial :ASV @19:34:38.63 ASV.dial :wasteR @19:34:39.12 AS.seek 0.000ml @19:34:41.84 Stroke completed -- waiting for pressure to equalize ... @19:34:42.49 Intake.select :close @19:34:42.64 SSV.dial :exhaust @19:34:43.39 Exhaust.select :open @19:34:43.50 SS.reconfigure SSexhale @19:34:43.54 SS.seek 0.00ml @19:34:52.48 Exhaust.select :close @19:34:53.09 CBV.dial SSV @19:34:53.10 SSV.dial CBV @19:34:53.88 Allowing 185s for 25.0ml of remaining 75.0ml @19:34:53.89 Intake.select :open @19:34:56.02 Intake @34.5psia, vacuum from 20.0 to 24.0psi @19:34:56.04 SS.reconfigure forSampling @19:34:56.07 SS.seek 25.00ml @19:35:26.39 Stroke completed -- waiting for pressure to equalize ... @19:35:27.03 Intake.select :close @19:35:27.18 SSV.dial :exhaust @19:35:27.93 Exhaust.select :open @19:35:28.03 SS.reconfigure SSexhale @19:35:28.07 SS.seek 0.00ml @19:35:36.62 Exhaust.select :close @19:35:36.72 SSV.dial CBV @19:35:36.74 CBV.dial SSV @19:35:37.50 Allowing 185s for 25.0ml of remaining 50.0ml @19:35:37.52 Intake.select :open @19:35:39.65 Intake @34.5psia, vacuum from 20.0 to 24.0psi @19:35:39.67 SS.reconfigure forSampling @19:35:39.70 SS.seek 25.00ml @19:36:10.06 Stroke completed -- waiting for pressure to equalize ... @19:36:10.70 Intake.select :close @19:36:10.85 SSV.dial :exhaust @19:36:11.60 Exhaust.select :open @19:36:11.70 SS.reconfigure SSexhale @19:36:11.74 SS.seek 0.00ml @19:36:20.17 Exhaust.select :close @19:36:20.27 SSV.dial CBV @19:36:20.29 CBV.dial SSV @19:36:21.04 Allowing 185s for 25.0ml of remaining 25.0ml @19:36:21.06 Intake.select :open @19:36:23.19 Intake @34.4psia, vacuum from 20.0 to 24.0psi @19:36:23.21 SS.reconfigure forSampling @19:36:23.24 SS.seek 24.96ml @19:36:39.53 delay 1:00 @19:36:53.56 Stroke completed -- waiting for pressure to equalize ... @19:36:54.20 Intake.select :close @19:36:54.34 SSV.dial :exhaust @19:36:55.10 Exhaust.select :open @19:36:55.21 SS.reconfigure SSexhale @19:36:55.25 SS.seek 0.00ml @19:37:03.77 Exhaust.select :close @19:37:03.87 Sampled 1000.0ml @19:37:03.88 collectSample: Complete @19:37:03.89 Drying puck before fixing for 04:00.00 @19:37:03.90 CBV.dial :waste2b @19:37:04.64 delay 1:00 @19:37:39.55 AFV.select :carrierSolution @19:37:39.64 delay 2 seconds @19:37:41.65 AS.reconfigure ASconfig @19:37:41.68 AS.seek 1.000ml @19:38:04.66 CSV.dial :air @19:38:05.11 CS.reconfigure CSfast @19:38:05.14 CS.seek 10.000ml @19:38:28.38 CBV.dial between CSV and waste2b @19:38:28.71 Pumping wcr puck from tube 2 up to 55psi... @19:38:28.76 CBV.dial SSV @19:38:30.18 CBV.dial between CSV and waste2b @19:38:31.13 CSV.dial between air and CTV @19:38:31.53 CS.seek 6.963ml @19:38:39.23 CTV.dial CSV,avoiding => air @19:38:39.25 CSV.dial CTV @19:38:39.99 CS.reconfigure CSpressurize @19:38:40.02 CS.seek 0.000ml @19:38:40.27 delay 1 second @19:38:41.28 AFV.select :holdingCoil @19:38:41.37 delay 1 second @19:38:42.38 ATV.dial :ASV @19:38:42.39 ASV.dial :wasteR @19:38:42.40 AS.reconfigure ASslow1 @19:38:42.44 AS.seek 0.000ml @19:38:57.93 CBV.dial :waste2b @19:38:58.26 CS.reconfigure CSconfig @19:38:58.29 CS.seek empty @19:38:58.97 delay 1:00 @19:39:58.98 CTV.dial :air,avoiding => intake @19:40:00.26 Fix #1 of 2 (with rnal) for 02:00.00 @19:40:00.27 CS Pulling 2ml of rnal @19:40:00.28 CSV.dial CSR @19:40:00.70 CSR.select :rnal @19:40:00.80 CS.seek 2.000ml @19:40:06.75 delay 5 seconds @19:40:11.76 CSR.select :air @19:40:11.85 CS.reconfigure CSfast @19:40:11.89 CS.seek 10.000ml @19:40:30.68 CBV.dial between CSV and waste2b @19:40:31.01 Pumping wcr puck from tube 2 up to 55psi... @19:40:31.06 CBV.dial SSV @19:40:32.49 CBV.dial between CSV and waste2b @19:40:33.44 CSV.dial between air and CTV @19:40:34.10 CS.seek 6.878ml @19:40:41.97 CTV.dial CSV,avoiding => air @19:40:41.99 CSV.dial CTV @19:40:43.30 CS.reconfigure CSpressurize @19:40:43.33 CS.seek 0.000ml @19:41:00.61 delay 2:00 @19:42:06.68 delay 20 seconds @19:42:26.69 ATV.dial :air3 @19:42:28.31 AS.seek 0.590ml @19:43:00.62 CBV.dial :waste2b @19:43:00.95 CS.reconfigure CSconfig @19:43:00.98 CS.seek empty @19:43:02.39 delay 1:00 @19:44:02.40 CTV.dial :air,avoiding => intake @19:44:03.68 Fix #2 of 2 (with rnal) for 02:00.00 @19:44:03.69 CS Pulling 2ml of rnal CSV.dial CSR @19:44:04.11 CSR.select :rnal @19:44:04.21 CS.seek 2.000ml @19:44:10.14 delay 5 seconds @19:44:15.15 CSR.select :air @19:44:15.24 CS.reconfigure CSfast @19:44:15.28 CS.seek 10.000ml @19:44:28.93 delay 3 seconds @19:44:31.94 ATV.dial :ASV @19:44:33.01 ASV.dial :wasteR @19:44:33.07 AS.seek 0.090ml @19:44:34.02 CBV.dial between CSV and waste2b @19:44:34.35 Pumping wcr puck from tube 2 up to 55psi... @19:44:34.40 CBV.dial SSV @19:44:35.84 CBV.dial between CSV and waste2b @19:44:36.78 CSV.dial between air and CTV @19:44:38.26 CS.seek 6.783ml @19:44:46.34 CTV.dial CSV,avoiding => air @19:44:46.36 CSV.dial CTV @19:44:47.66 CS.reconfigure CSpressurize @19:44:47.69 CS.seek 0.000ml @19:45:04.72 delay 2:00 @19:46:15.29 delay 30 seconds @19:46:45.30 ATV.dial :extSrc @19:46:45.82 delay 3 seconds @19:46:48.83 ATV.dial :ElutionWater @19:46:49.85 AS.seek 0.120ml @19:46:56.22 ATV.dial :air3 @19:46:56.58 AS.seek 0.130ml @19:46:58.88 delay 2 seconds @19:47:00.89 ATV.dial :bleach @19:47:01.27 AS.seek 0.230ml @19:47:04.73 CBV.dial :waste2b @19:47:05.05 CS.reconfigure CSconfig @19:47:05.08 CS.seek empty @19:47:06.54 delay 1:00 @19:47:21.91 delay 3 seconds @19:47:24.92 ATV.dial :air3 @19:47:25.35 delay 3 seconds @19:47:28.41 AS.seek 0.255ml @19:47:33.74 delay 3 seconds @19:47:36.75 ATV.dial :bleach @19:47:37.13 AS.seek 0.240ml @19:47:40.44 delay 3 seconds @19:47:43.45 ATV.dial :ASV @19:47:44.33 ASV.dial :SPEdelivery @19:47:44.80 AFV.select :carrierSolution @19:47:44.90 delay 2 seconds @19:47:46.91 AS.reconfigure ASconfig @19:47:46.94 AS.seek 0.500ml @19:48:02.36 delay 1 second @19:48:03.37 AFV.select :holdingCoil @19:48:03.51 AS.reconfigure ASslow1 @19:48:03.54 AS.seek 0.170ml @19:48:06.55 CTV.dial :air,avoiding => intake @19:48:07.87 Storing wcr puck in Collection Clamp to tube 1 @19:48:08.00 SC.reconfigure SCtight @19:48:08.03 SC.seek 1 @19:48:10.26 SC.reconfigure SCloose @19:48:10.44 Elbow.reconfigure TightElbow @19:48:10.47 Elbow.seek 1 @19:48:12.15 Forearm.reconfigure TightForearm @19:48:12.18 Forearm.seek 1 + 800 counts @19:48:13.29 SE.reconfigure SEpick @19:48:13.34 SE.seek beamBreak @19:48:16.33 SE.configure SEconfig @19:48:16.45 Elbow.reconfigure LimpElbow @19:48:16.49 Forearm.reconfigure LooseForearm @19:48:16.53 Forearm.seek 1 @19:48:16.77 Elbow.hold @19:48:16.82 Elbow.reconfigure TightElbow @19:48:16.90 SE.seek 1.04 Pucks below Top Plate @19:48:17.21 Forearm.coast @19:48:17.24 Forearm.seek 1 + 346 counts @19:48:17.48 Forearm.reconfigure TightForearm @19:48:17.52 Forearm.seek 1 + 800 counts @19:48:17.81 SE.seek 2.04 Pucks below Top Plate @19:48:18.91 Forearm.reconfigure LooseForearm @19:48:18.95 Forearm.seek retract @19:48:19.84 Elbow.seek CC @19:48:21.35 Elbow.reconfigure LooseElbow @19:48:21.43 Forearm.seek CC @19:48:22.42 CC.seek sealed @19:48:23.32 Hand.grip @19:48:24.06 CC.seek open @19:48:27.44 Forearm.seek retract @19:48:28.39 Elbow.reconfigure TightElbow @19:48:28.42 Elbow.seek 1 @19:48:30.01 Elbow.reconfigure LimpElbow @19:48:30.05 Forearm.seek 1 @19:48:31.08 Elbow.hold @19:48:31.13 Elbow.reconfigure TightElbow @19:48:31.17 Hand.release @19:48:32.02 Forearm.coast @19:48:32.06 Forearm.seek 1 + 315 counts @19:48:32.29 Forearm.reconfigure TightForearm @19:48:32.33 Forearm.seek 1 + 800 counts @19:48:32.54 SE.seek down @19:48:33.65 Forearm.reconfigure LooseForearm @19:48:33.68 Forearm.seek retract @19:48:35.10 SE.coast @19:48:35.13 SC.coast @19:48:35.16 Starting finalPurge @19:48:35.17 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @19:48:35.31 Elbow.seek FlushPuck::Garage @19:48:37.05 Elbow.reconfigure LooseElbow @19:48:37.13 Forearm.seek garage @19:48:38.16 Hand.grip @19:48:39.03 Forearm.seek retract @19:48:40.03 Elbow.reconfigure TightElbow @19:48:40.06 Elbow.seek CC @19:48:42.79 Elbow.reconfigure LooseElbow @19:48:42.87 Forearm.reconfigure TightForearm @19:48:42.90 Forearm.seek CC @19:48:43.99 CC.seek closed @19:48:47.78 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 2.04 @19:48:47.83 Hand.release @19:48:48.60 Forearm.reconfigure LooseForearm @19:48:48.63 Forearm.seek retract @19:48:49.61 Flushing the Collection Valve::Manifold @19:48:49.71 CS Pulling 5ml of flush CSV.dial CSR @19:48:50.13 CSR.select :flush @19:48:50.23 CS.seek 5.000ml @19:49:03.81 delay 5 seconds @19:49:08.82 CSR.select :air @19:49:08.96 CS.reconfigure CSfast @19:49:08.99 CS.seek 7.500ml @19:49:11.11 SPE.hold 2:46:40,95.0C @19:49:11.13 delay 3:00 @19:49:15.63
CSV.dial :waste @19:49:16.95 CS.reconfigure CSconfig @19:49:16.98 CS.seek 0.000ml @19:49:36.81 Flushing lower manifold's waste1 port @19:49:37.51 CSV.dial :flush @19:49:37.96 CS.reconfigure CSfast @19:49:37.99 CS.seek 5.000ml @19:49:50.66 delay 5 seconds @19:49:55.67 CSV.dial :air @19:49:56.16 CS.seek 7.000ml @19:50:01.69 CSV.dial CTV @19:50:01.71
CTV.dial CSV @19:50:03.09 CBV.dial :waste1 @19:50:03.87 CS.seek 0.000ml @19:50:21.19 Flushing lower manifold's waste2b port @19:50:21.28 CSV.dial :flush @19:50:22.12 CS.seek 5.000ml @19:50:34.81 delay 5 seconds @19:50:39.82 CSV.dial :air @19:50:40.39 CS.seek 7.000ml @19:50:45.90 CSV.dial CTV @19:50:45.92
CTV.dial CSV @19:50:46.60 CBV.dial :waste2b @19:50:47.37 CS.seek 0.000ml @19:51:04.65 Flushing upper manifold @19:51:04.74 CSV.dial :flush @19:51:05.49 CS.seek 5.000ml @19:51:18.06 delay 5 seconds @19:51:23.07 CSV.dial :air @19:51:23.65 CS.seek 7.000ml @19:51:29.18 CSV.dial CBV @19:51:29.20
CBV.dial CSV @19:51:30.37 CTV.dial :air @19:51:31.71 CS.seek 0.000ml @19:51:49.03 delay 7 seconds @19:51:56.04 CS.seek 3.000ml @19:52:03.93 CSV.dial :waste @19:52:04.46 CS.seek 0.000ml @19:52:11.14 AFV.select :carrierSolution @19:52:11.23 delay 2 seconds @19:52:12.46
CSV.dial :flush @19:52:12.91 CS.seek 3.000ml @19:52:13.24 AS.reconfigure ASconfig @19:52:13.28 AS.seek 1.000ml @19:52:20.77
delay 5 seconds @19:52:25.78 CSV.dial :air @19:52:26.26 CS.seek 5.000ml @19:52:31.77 CSV.dial :air @19:52:31.79 CS.seek 0.000ml @19:52:44.21 delay 7 seconds @19:52:51.22 CS.seek 3.000ml @19:52:59.05 CSV.dial :waste @19:52:59.86 CS.seek 0.000ml @19:53:01.96 delay 1 second @19:53:02.97 AFV.select :holdingCoil @19:53:03.09 delay 1 second @19:53:04.10 ASV.dial :wasteD @19:53:05.11 AS.seek 0.000ml @19:53:07.65
Disinfecting external Intake with mfbkill @19:53:07.74 CSV.dial CSR @19:53:09.12 CSR.select :mfbkill @19:53:09.22 CS.seek 8.000ml @19:53:28.55 delay 5 seconds @19:53:33.56 CSR.select :air @19:53:33.65 CS.seek 10.000ml @19:53:39.18 CSV.dial CBV @19:53:39.20
CBV.dial CSV @19:53:40.21 CTV.dial :intake @19:53:40.93 Intake.select :open @19:53:41.04 CS.seek 2.000ml @19:54:00.83 Intake.select :close @19:54:00.92 CTV.dial :air @19:54:01.69 CS.reconfigure CSconfig @19:54:01.73 CS.seek 0.000ml @19:54:03.67 Priming Eluate @19:54:03.68 AFV.select :carrierSolution @19:54:03.77 delay 2 seconds @19:54:05.78 AS.seek 0.500ml @19:54:07.58
Flushing lower manifold's waste1 port @19:54:07.59 CS Pulling 4ml of mfbkill @19:54:07.60 CSV.dial CSR @19:54:08.68 CSR.select :mfbkill @19:54:08.78 CS.seek 4.000ml @19:54:19.74 delay 5 seconds @19:54:24.75 CSR.select :air @19:54:24.84 CS.reconfigure CSfast @19:54:24.88 CS.seek 10.000ml @19:54:35.19 delay 1 second @19:54:36.21 AFV.select :holdingCoil @19:54:36.30 ATV.dial ASV @19:54:36.31 ASV.dial :SPErecovery @19:54:37.23 Swish #1 of 2 @19:54:37.29 AS.reconfigure ASslow0 @19:54:37.32 AS.seek 0.600ml @19:54:39.41 CSV.dial CTV @19:54:39.43
CTV.dial CSV @19:54:40.78 CBV.dial :waste1 @19:54:41.24 CS.seek 0.000ml @19:54:47.86 AS.seek 0.500ml @19:54:58.85 Swish #2 of 2 @19:54:58.91 AS.seek 0.600ml @19:55:04.65
Flushing lower manifold's waste2b port @19:55:04.66 CS Pulling 4ml of mfbkill @19:55:04.67 CSV.dial CSR @19:55:05.18 CSR.select :mfbkill @19:55:05.28 CS.reconfigure CSconfig @19:55:05.32 CS.seek 4.000ml @19:55:09.48 AS.seek 0.500ml @19:55:16.25
delay 5 seconds @19:55:19.96 delay 3 seconds @19:55:21.26
CSR.select :air @19:55:21.35 CS.reconfigure CSfast @19:55:21.39 CS.seek 10.000ml @19:55:23.02 AS.reconfigure ASslow1 @19:55:23.05 AS.seek 0.676ml @19:55:35.79 CSV.dial CTV @19:55:35.81
CTV.dial CSV @19:55:36.33 CBV.dial :waste2b @19:55:37.08 CS.seek 0.000ml @19:55:59.21 delay 3 seconds @19:56:00.46
Killing upper manifold @19:56:00.47 CS Pulling 4ml of mfbkill @19:56:00.48 CSV.dial CSR @19:56:00.99 CSR.select :mfbkill @19:56:01.09 CS.reconfigure CSconfig @19:56:01.13 CS.seek 4.000ml @19:56:02.22 Eluate primed @19:56:02.23 ATV.dial :waste2 @19:56:02.85 AS.reconfigure ASnorm @19:56:02.88 AS.seek 0.000ml @19:56:12.12
delay 5 seconds @19:56:17.13 CSR.select :air @19:56:17.22 CS.reconfigure CSfast @19:56:17.26 CS.seek 10.000ml @19:56:31.68 CSV.dial CBV @19:56:31.70
CBV.dial CSV @19:56:33.13 CTV.dial :air @19:56:34.44 CS.seek 0.000ml @19:56:37.73 SPE.coast @19:56:37.80 ASV.dial :SPErecovery @19:56:37.82 Setting up PCR for HF183em -- writing /var/log/esp/HF183em14feb1119h1000ml.pcr @19:56:37.83 Building PCR Slug from ASV with MM2 mix, PP4 primer @19:56:37.84 Priming with carrier @19:56:37.85 ATV.dial ARV @19:56:38.63 ARV.dial :carrier @19:56:38.92 AS.seek 0.500ml @19:56:57.71
delay 7 seconds @19:57:04.72 CS.seek 3.000ml @19:57:04.85 AFV.select :carrierSolution @19:57:04.95 delay 2 seconds @19:57:06.96 AS.reconfigure ASconfig @19:57:06.99 AS.seek 1.000ml @19:57:12.33
CSV.dial :waste @19:57:12.98 CS.seek 0.000ml @19:57:20.49 Flushing upper manifold @19:57:20.58 CSV.dial :flush @19:57:21.11 CS.seek 5.000ml @19:57:33.11 delay 5 seconds @19:57:36.44 delay 1 second @19:57:37.45 AFV.select :holdingCoil @19:57:37.55 delay 1 second @19:57:38.12
CSV.dial :air @19:57:38.58 CS.seek 7.000ml @19:57:38.60 ATV.dial :waste @19:57:39.87 AS.reconfigure ASnorm @19:57:39.90 AS.seek empty @19:57:43.96 CSV.dial CBV @19:57:43.98
CBV.dial CSV @19:57:45.05 CTV.dial :air @19:57:45.07 CS.seek 0.000ml @19:58:01.67 delay 7 seconds @19:58:08.68 CS.seek 3.000ml @19:58:16.24 CSV.dial :waste @19:58:16.75 CS.seek 0.000ml @19:58:24.35 CSV.dial :flush @19:58:24.79 CS.seek 3.000ml @19:58:31.38 AFV.select :carrierSolution @19:58:31.49 delay 2 seconds @19:58:32.33
delay 5 seconds @19:58:33.50 AS.reconfigure ASconfig @19:58:33.54 AS.seek 0.500ml @19:58:37.34
CSV.dial :air @19:58:37.82 CS.seek 4.999ml @19:58:43.20 CSV.dial :air @19:58:43.22 CS.seek 0.000ml @19:58:55.17 delay 7 seconds @19:59:02.18 CS.seek 3.000ml @19:59:03.02 delay 1 second @19:59:04.03 AFV.select :holdingCoil @19:59:04.13 Building Slug @19:59:04.14 ATV.dial :ARV @19:59:05.47 delay 2 seconds @19:59:07.48 ARV.dial :air1 @19:59:07.80 delay 2 seconds @19:59:09.73
CSV.dial :waste @19:59:09.86 AS.reconfigure ASslow2 @19:59:09.89 AS.seek 0.506ml @19:59:10.53
CS.seek 0.000ml @19:59:16.39 delay 2 seconds @19:59:18.18
Flushing lower manifold's waste1 port @19:59:18.26 CSV.dial :flush @19:59:18.40 ARV.dial :MM2 @19:59:18.78
CS.seek 5.000ml @19:59:20.16 delay 2 seconds @19:59:22.17 Adding MM2 @19:59:22.23 AS.seek 0.524ml @19:59:30.73
delay 5 seconds @19:59:35.74 CSV.dial :air @19:59:36.20 CS.seek 7.000ml @19:59:41.02 delay 25 seconds @19:59:41.61 CSV.dial CTV @19:59:41.63
CTV.dial CSV @19:59:43.01 CBV.dial :waste1 @19:59:43.46 CS.seek 0.000ml @19:59:59.92 Flushing lower manifold's waste2b port @20:00:00.01 CSV.dial :flush @20:00:00.73 CS.seek 5.000ml @20:00:06.03 ARV.dial :PP4 @20:00:06.36 Adding PP4 @20:00:06.37 delay 2 seconds @20:00:08.43 AS.seek 0.530ml @20:00:12.79
delay 5 seconds @20:00:14.86 delay 10 seconds @20:00:17.80
CSV.dial :air @20:00:18.34 CS.seek 7.001ml @20:00:23.64 CSV.dial CTV @20:00:23.66
CTV.dial CSV @20:00:24.32 CBV.dial :waste2b @20:00:24.87 ARV.dial :air3 @20:00:25.07
CS.seek 0.000ml @20:00:25.20 adding ARV air @20:00:25.21 delay 2 seconds @20:00:27.26 AS.seek 0.536ml @20:00:33.51 delay 5 seconds @20:00:38.52 ARV.dial :carrier @20:00:39.55 delay 7 seconds @20:00:41.48
Disinfecting external Intake with kill @20:00:41.57 CSV.dial CSR @20:00:42.09 CSR.select :kill @20:00:42.20 CS.seek 8.000ml @20:00:46.61 AS.seek 0.594ml @20:01:00.98
delay 5 seconds @20:01:05.99 CSR.select :air @20:01:06.08 CS.seek 10.000ml @20:01:11.48 CSV.dial CBV @20:01:11.50
CBV.dial CSV @20:01:12.52 CTV.dial :intake @20:01:13.23 Intake.select :open @20:01:13.32 CS.seek 2.000ml @20:01:32.79 Intake.select :close @20:01:32.88 CTV.dial :air @20:01:33.60 CS.reconfigure CSconfig @20:01:33.64 CS.seek 0.000ml @20:01:39.73 CSV.dial CSR @20:01:40.77 CSR.select :flush @20:01:40.87 CS.seek 5.000ml @20:01:45.90 delay 5 seconds @20:01:50.91 ATV.dial :ASV @20:01:51.23 delay 2 seconds @20:01:53.29 AS.seek 0.600ml @20:01:54.26
delay 5 seconds @20:01:59.27 CSR.select :air @20:01:59.36 CS.seek 10.000ml @20:01:59.53 delay 4 seconds @20:02:03.54 ATV.dial ARV @20:02:03.87 delay 2 seconds @20:02:05.88 ARV.dial :carrier @20:02:05.89 delay 2 seconds @20:02:07.94 AS.seek 0.650ml @20:02:12.79
CSV.dial CBV @20:02:13.84 CS.seek 0.000ml @20:02:39.95 Garaging FlushPuck in Collection Clamp @20:02:40.01 Elbow.reconfigure TightElbow @20:02:40.04 Elbow.seek CC @20:02:40.13 Elbow.reconfigure LooseElbow @20:02:40.22 Forearm.seek CC @20:02:41.17 CC.seek sealed @20:02:42.07 Hand.grip @20:02:42.78 CC.seek open @20:02:46.10 Forearm.seek retract @20:02:47.05 Elbow.reconfigure TightElbow @20:02:47.08 Elbow.seek garage @20:02:49.87 Elbow.reconfigure LooseElbow @20:02:49.95 Forearm.reconfigure TightForearm @20:02:49.99 Forearm.seek garage @20:02:51.16 Hand.release @20:02:51.95 Forearm.reconfigure LooseForearm @20:02:51.98 Forearm.seek retract @20:02:59.26 delay 4 seconds @20:03:03.27 ATV.dial PCR @20:03:03.78 ASV.dial :waste1 @20:03:05.17 ARV.dial :air1 @20:03:05.60 Positioning Slug by 0.324ml @20:03:05.66 AS.seek 0.326ml @20:08:36.33 delay 5 seconds @20:08:41.34 ATV.dial ARV @20:08:41.86 ASV.dial :PCRlock @20:08:42.64 delay 5 seconds @20:08:47.65 Running HF183em ProfileEM60C2 thermocycling @20:08:47.66 PCR.program ProfileEM60C2 @20:08:47.77 ProfileEM60C2 should complete in < 2:11:38 @20:08:47.78 PCR.run 2:49:32.5 @20:35:57.94 Session Begun @20:36:11.19 -> showlog @20:37:03.27 -> exit @20:37:03.28 Session Ended @22:08:12.40 PCR.off @22:08:12.93 PCR for HF183em completed -- closed /var/log/esp/HF183em14feb1119h1000ml.pcr @22:08:12.98 Cleaning PCR AFV.select :carrierSolution @22:08:13.08 delay 2 seconds @22:08:15.09 AS.reconfigure ASconfig @22:08:15.12 AS.seek 0.500ml @22:08:25.54 delay 1 second @22:08:26.55 AFV.select :holdingCoil @22:08:26.64 ASV.dial :waste1 @22:08:27.32 ATV.dial :air3 @22:08:28.26 AS.reconfigure ASslow1 @22:08:28.29 AS.seek 0.510ml @22:08:30.58 delay 2 seconds @22:08:32.59 ATV.dial :bleach @22:08:32.96 AS.seek 0.810ml @22:09:34.38 delay 3 seconds @22:09:37.39 ATV.dial :air3 @22:09:37.78 delay 3 seconds @22:09:40.84 AS.seek 0.835ml @22:09:46.16 delay 3 seconds @22:09:49.17 ATV.dial :bleach @22:09:49.55 AS.seek 0.820ml @22:09:52.88 delay 3 seconds @22:09:55.89 ARV.dial :waste @22:09:56.94 delay 3 seconds @22:09:59.95 ATV.dial :ARV @22:10:00.69 AS.reconfigure ASconfig @22:10:00.72 AS.seek 0.470ml @22:10:21.39 AFV.select :carrierSolution @22:10:21.49 delay 2 seconds @22:10:23.50 AS.seek 1.000ml @22:10:54.73 delay 1 second @22:10:55.74 AFV.select :holdingCoil @22:10:55.83 delay 1 second @22:10:56.84 AS.reconfigure ASfast @22:10:56.88 AS.seek 0.000ml @22:11:24.65 AFV.select :carrierSolution @22:11:24.75 delay 2 seconds @22:11:26.76 AS.reconfigure ASconfig @22:11:26.79 AS.seek 1.000ml @22:12:25.40 delay 1 second @22:12:26.41 AFV.select :holdingCoil @22:12:26.50 delay 1 second @22:12:27.51 AS.reconfigure ASfast @22:12:27.54 AS.seek 0.000ml @22:12:55.29 AFV.select :carrierSolution @22:12:55.39 delay 2 seconds @22:12:57.40 AS.reconfigure ASconfig @22:12:57.43 AS.seek 1.000ml @22:13:56.02 delay 1 second @22:13:57.03 AFV.select :holdingCoil @22:13:57.12 delay 1 second @22:13:58.13 AS.reconfigure ASfast @22:13:58.16 AS.seek 0.000ml @22:14:25.89 ARV.dial between carrier and air1 @22:14:26.30 ATV.dial :air3 @22:14:27.22 AS.reconfigure ASslow1 @22:14:27.25 AS.seek 0.010ml @22:14:29.60 delay 2 seconds @22:14:31.61 ATV.dial :bleach @22:14:31.99 AS.seek 0.810ml @22:17:15.39 delay 3 seconds @22:17:18.40 ATV.dial :air3 @22:17:18.82 delay 3 seconds @22:17:21.88 AS.seek 0.835ml @22:17:27.23 delay 3 seconds @22:17:30.24 ATV.dial :bleach @22:17:30.61 AS.seek 0.820ml @22:17:33.92 delay 3 seconds @22:17:36.93 ATV.dial PCR @22:17:37.27 AS.reconfigure ASconfig @22:17:37.30 AS.seek empty @22:18:25.42 PCR.program :bleachProfile @22:18:25.48 PCR program should complete in < 2:45 @22:18:25.49 PCR.run 10:00 @22:21:20.66 PCR.off @22:21:20.69 AFV.select :carrierSolution @22:21:20.78 delay 2 seconds @22:21:22.79 AS.seek 1.000ml @22:22:21.45 delay 1 second @22:22:22.46 AFV.select :holdingCoil @22:22:22.55 delay 1 second @22:22:23.56 AS.reconfigure ASfast @22:22:23.59 AS.seek 0.000ml @22:22:31.75 Session Begun @22:22:42.51 -> showlog @22:22:51.33 delay 3 seconds @22:22:54.34 AFV.select :carrierSolution @22:22:54.44 delay 2 seconds @22:22:56.45 AS.reconfigure ASconfig @22:22:56.50 AS.seek 1.000ml @22:23:29.19 -> exit @22:23:29.20 Session Ended @22:23:55.12 delay 1 second @22:23:56.13 AFV.select :holdingCoil @22:23:56.22 delay 1 second @22:23:57.23 AS.reconfigure ASfast @22:23:57.26 AS.seek 0.000ml @22:24:25.01 delay 3 seconds @22:24:28.02 AFV.select :carrierSolution @22:24:28.11 delay 2 seconds @22:24:30.12 AS.reconfigure ASconfig @22:24:30.15 AS.seek 1.000ml @22:25:28.75 delay 1 second @22:25:29.76 AFV.select :holdingCoil @22:25:29.85 delay 1 second @22:25:30.86 AS.reconfigure ASfast @22:25:30.89 AS.seek 0.000ml @22:25:58.63 delay 3 seconds @22:26:01.64 AFV.select :carrierSolution @22:26:01.73 delay 2 seconds @22:26:03.74 AS.reconfigure ASconfig @22:26:03.77 AS.seek 1.000ml @22:27:02.40 delay 1 second @22:27:03.41 AFV.select :holdingCoil @22:27:03.50 delay 1 second @22:27:04.51 AS.reconfigure ASfast @22:27:04.54 AS.seek 0.000ml @22:27:32.27 delay 3 seconds @22:27:35.28 AFV.select :carrierSolution @22:27:35.37 delay 2 seconds @22:27:37.38 AS.reconfigure ASconfig @22:27:37.41 AS.seek 1.000ml @22:28:36.03 delay 1 second @22:28:37.04 AFV.select :holdingCoil @22:28:37.13 delay 1 second @22:28:38.14 AS.reconfigure ASnorm @22:28:38.17 AS.seek 0.000ml @22:29:29.60 Setting up PCR for ENTap -- writing /var/log/esp/ENTap14feb1122h1000ml.pcr @22:29:29.61 Building PCR Slug from ASV with MM1 mix, PP1 primer @22:29:29.62 Priming with carrier @22:29:29.63 ATV.dial ARV @22:29:30.12 ARV.dial :carrier @22:29:30.41 AS.seek 0.500ml @22:29:56.28 AFV.select :carrierSolution @22:29:56.37 delay 2 seconds @22:29:58.38 AS.reconfigure ASconfig @22:29:58.41 AS.seek 1.000ml @22:30:27.89 delay 1 second @22:30:28.90 AFV.select :holdingCoil @22:30:28.99 delay 1 second @22:30:30.00 ATV.dial :waste @22:30:31.35 AS.reconfigure ASnorm @22:30:31.38 AS.seek empty @22:31:22.85 AFV.select :carrierSolution @22:31:22.95 delay 2 seconds @22:31:24.96 AS.reconfigure ASconfig @22:31:24.99 AS.seek 0.500ml @22:31:54.48 delay 1 second @22:31:55.49 AFV.select :holdingCoil @22:31:55.58 Building Slug @22:31:55.59 ATV.dial :ARV @22:31:56.90 delay 2 seconds @22:31:58.91 ARV.dial :air1 @22:31:59.24 delay 2 seconds @22:32:01.30 AS.reconfigure ASslow2 @22:32:01.33 AS.seek 0.506ml @22:32:08.30 delay 2 seconds @22:32:10.31 ARV.dial :MM1 @22:32:10.88 delay 2 seconds @22:32:12.89 Adding MM1 @22:32:12.95 AS.seek 0.524ml @22:32:31.41 delay 25 seconds @22:32:56.42 ARV.dial :PP1 @22:32:56.72 Adding PP1 @22:32:56.74 delay 2 seconds @22:32:58.79 AS.seek 0.530ml @22:33:05.50 delay 10 seconds @22:33:15.51 ARV.dial :air1 @22:33:15.82 adding ARV air @22:33:15.83 delay 2 seconds @22:33:17.89 AS.seek 0.536ml @22:33:24.21 delay 5 seconds @22:33:29.22 ARV.dial :carrier @22:33:29.54 delay 7 seconds @22:33:36.60 AS.seek 0.594ml @22:34:36.31 delay 5 seconds @22:34:41.32 ATV.dial :ASV @22:34:41.72 delay 2 seconds @22:34:43.78 AS.seek 0.600ml @22:34:50.06 delay 4 seconds @22:34:54.07 ATV.dial ARV @22:34:54.39 delay 2 seconds @22:34:56.40 ARV.dial :carrier @22:34:56.41 delay 2 seconds @22:34:58.46 AS.seek 0.650ml @22:35:49.56 delay 4 seconds @22:35:53.57 ATV.dial PCR @22:35:54.06 ASV.dial :waste1 @22:35:54.07 ARV.dial :air1 @22:35:54.42 Positioning Slug by 0.324ml @22:35:54.47 AS.seek 0.326ml @22:41:25.27 delay 5 seconds @22:41:30.28 ATV.dial ARV @22:41:30.80 ASV.dial :PCRlock @22:41:31.32 delay 5 seconds @22:41:36.33 Running ENTap Profile60C thermocycling @22:41:36.34 PCR.program Profile60C @22:41:36.44 Profile60C should complete in < 1:39:08 @22:41:36.45 PCR.run 2:08:55 @23:06:47.29 Session Begun @23:06:56.60 -> showlog @23:09:36.61 -> showlog 50 @23:09:52.37 -> exit @23:09:52.38 Session Ended @00:08:10.24PST12-Feb-14 PCR.off @00:08:10.76 PCR for ENTap completed -- closed /var/log/esp/ENTap14feb1122h1000ml.pcr @00:08:10.81 Cleaning PCR @00:08:10.82 AFV.select :carrierSolution @00:08:10.91 delay 2 seconds @00:08:12.92 AS.reconfigure ASconfig @00:08:12.96 AS.seek 0.500ml @00:08:23.37 delay 1 second @00:08:24.38 AFV.select :holdingCoil @00:08:24.47 ASV.dial :waste1 @00:08:25.12 ATV.dial :air3 @00:08:26.05 AS.reconfigure ASslow1 @00:08:26.08 AS.seek 0.510ml @00:08:28.37 delay 2 seconds @00:08:30.38 ATV.dial :bleach @00:08:30.75 AS.seek 0.810ml @00:09:32.18 delay 3 seconds @00:09:35.19 ATV.dial :air3 @00:09:35.60 delay 3 seconds @00:09:38.66 AS.seek 0.835ml @00:09:44.01 delay 3 seconds @00:09:47.02 ATV.dial :bleach @00:09:47.40 AS.seek 0.820ml @00:09:50.71 delay 3 seconds @00:09:53.72 ARV.dial :waste @00:09:54.31 delay 3 seconds @00:09:57.32 ATV.dial :ARV @00:09:58.06 AS.reconfigure ASconfig @00:09:58.09 AS.seek 0.470ml @00:10:18.76 AFV.select :carrierSolution @00:10:18.86 delay 2 seconds @00:10:20.87 AS.seek 1.000ml @00:10:52.05 delay 1 second @00:10:53.06 AFV.select :holdingCoil @00:10:53.15 delay 1 second @00:10:54.16 AS.reconfigure ASfast @00:10:54.19 AS.seek 0.000ml @00:11:21.89 AFV.select :carrierSolution @00:11:21.99 delay 2 seconds @00:11:24.00 AS.reconfigure ASconfig @00:11:24.03 AS.seek 1.000ml @00:12:22.65 delay 1 second @00:12:23.66 AFV.select :holdingCoil @00:12:23.75 delay 1 second @00:12:24.76 AS.reconfigure ASfast @00:12:24.79 AS.seek 0.000ml @00:12:52.50 AFV.select :carrierSolution @00:12:52.60 delay 2 seconds @00:12:54.61 AS.reconfigure ASconfig @00:12:54.64 AS.seek 1.000ml @00:13:53.23 delay 1 second @00:13:54.24 AFV.select :holdingCoil @00:13:54.33 delay 1 second @00:13:55.34 AS.reconfigure ASfast @00:13:55.37 AS.seek 0.000ml @00:14:23.04 ARV.dial between carrier and air1 @00:14:23.45 ATV.dial :air3 @00:14:24.39 AS.reconfigure ASslow1 @00:14:24.42 AS.seek 0.010ml @00:14:26.74 delay 2 seconds @00:14:28.75 ATV.dial :bleach @00:14:29.12 AS.seek 0.810ml @00:17:12.50 delay 3 seconds @00:17:15.51 ATV.dial :air3 @00:17:15.83 delay 3 seconds @00:17:18.89 AS.seek 0.835ml @00:17:24.24 delay 3 seconds @00:17:27.25 ATV.dial :bleach @00:17:27.64 AS.seek 0.820ml @00:17:30.96 delay 3 seconds @00:17:33.97 ATV.dial PCR @00:17:34.31 AS.reconfigure ASconfig @00:17:34.34 AS.seek empty @00:18:22.47 PCR.program :bleachProfile @00:18:22.53 PCR program should complete in < 2:45 @00:18:22.55 PCR.run 10:00 @00:21:17.74 PCR.off @00:21:17.77 AFV.select :carrierSolution @00:21:17.86 delay 2 seconds @00:21:19.87 AS.seek 1.000ml @00:22:18.51 delay 1 second @00:22:19.52 AFV.select :holdingCoil @00:22:19.61 delay 1 second @00:22:20.62 AS.reconfigure ASfast @00:22:20.65 AS.seek 0.000ml @00:22:48.38 delay 3 seconds @00:22:51.39 AFV.select :carrierSolution @00:22:51.48 delay 2 seconds @00:22:53.49 AS.reconfigure ASconfig @00:22:53.52 AS.seek 1.000ml @00:23:52.13 delay 1 second @00:23:53.14 AFV.select :holdingCoil @00:23:53.23 delay 1 second @00:23:54.24 AS.reconfigure ASfast @00:23:54.27 AS.seek 0.000ml @00:24:21.97 delay 3 seconds @00:24:24.98 AFV.select :carrierSolution @00:24:25.07 delay 2 seconds @00:24:27.08 AS.reconfigure ASconfig @00:24:27.11 AS.seek 1.000ml @00:25:25.72 delay 1 second @00:25:26.73 AFV.select :holdingCoil @00:25:26.82 delay 1 second @00:25:27.83 AS.reconfigure ASfast @00:25:27.91 AS.seek 0.000ml @00:25:55.64 delay 3 seconds @00:25:58.65 AFV.select :carrierSolution @00:25:58.74 delay 2 seconds @00:26:00.75 AS.reconfigure ASconfig @00:26:00.78 AS.seek 1.000ml @00:26:59.41 delay 1 second @00:27:00.42 AFV.select :holdingCoil @00:27:00.51 delay 1 second @00:27:01.52 AS.reconfigure ASfast @00:27:01.55 AS.seek 0.000ml @00:27:29.29 delay 3 seconds @00:27:32.30 AFV.select :carrierSolution @00:27:32.39 delay 2 seconds @00:27:34.40 AS.reconfigure ASconfig @00:27:34.93 AS.seek 1.000ml @00:28:33.49 delay 1 second @00:28:34.50 AFV.select :holdingCoil @00:28:34.59 delay 1 second @00:28:35.60 AS.reconfigure ASnorm @00:28:35.64 AS.seek 0.000ml @00:29:27.08 Setting up PCR for ENTem -- writing /var/log/esp/ENTem14feb1200h1000ml.pcr @00:29:27.09 Building PCR Slug from ASV with MM2 mix, PP3 primer @00:29:27.10 Priming with carrier @00:29:27.11 ATV.dial ARV @00:29:27.60 ARV.dial :carrier @00:29:27.90 AS.seek 0.500ml @00:29:53.77 AFV.select :carrierSolution @00:29:53.87 delay 2 seconds @00:29:55.88 AS.reconfigure ASconfig @00:29:55.92 AS.seek 1.000ml @00:30:25.38 delay 1 second @00:30:26.39 AFV.select :holdingCoil @00:30:26.48 delay 1 second @00:30:27.49 ATV.dial :waste @00:30:28.73 AS.reconfigure ASnorm @00:30:28.76 AS.seek empty @00:31:20.22 AFV.select :carrierSolution @00:31:20.32 delay 2 seconds @00:31:22.33 AS.reconfigure ASconfig @00:31:22.36 AS.seek 0.500ml @00:31:51.83 delay 1 second @00:31:52.84 AFV.select :holdingCoil @00:31:52.93 Building Slug @00:31:52.94 ATV.dial :ARV @00:31:54.17 delay 2 seconds @00:31:56.18 ARV.dial :air1 @00:31:56.51 delay 2 seconds @00:31:58.57 AS.reconfigure ASslow2 @00:31:58.60 AS.seek 0.506ml @00:32:04.86 delay 2 seconds @00:32:06.87 ARV.dial :MM2 @00:32:08.09 delay 2 seconds @00:32:10.10 Adding MM2 @00:32:10.16 AS.seek 0.524ml @00:32:28.83 delay 25 seconds @00:32:53.84 ARV.dial :PP3 @00:32:54.18 Adding PP3 @00:32:54.19 delay 2 seconds @00:32:56.24 AS.seek 0.530ml @00:33:02.90 delay 10 seconds @00:33:12.91 ARV.dial :air2 @00:33:13.33 adding ARV air @00:33:13.34 delay 2 seconds @00:33:15.39 AS.seek 0.536ml @00:33:21.78 delay 5 seconds @00:33:26.79 ARV.dial :carrier @00:33:27.82 delay 7 seconds @00:33:34.88 AS.seek 0.594ml @00:34:34.15 delay 5 seconds @00:34:39.16 ATV.dial :ASV @00:34:39.48 delay 2 seconds @00:34:41.54 AS.seek 0.600ml @00:34:47.82 delay 4 seconds @00:34:51.83 ATV.dial ARV @00:34:52.15 delay 2 seconds @00:34:54.16 ARV.dial :carrier @00:34:54.17 delay 2 seconds @00:34:56.22 AS.seek 0.650ml @00:35:47.46 delay 4 seconds @00:35:51.47 ATV.dial PCR @00:35:51.96 ASV.dial :waste1 @00:35:51.97 ARV.dial :air1 @00:35:52.31 Positioning Slug by 0.324ml @00:35:52.36 AS.seek 0.326ml @00:41:23.35 delay 5 seconds @00:41:28.36 ATV.dial ARV @00:41:28.95 ASV.dial :PCRlock @00:41:29.26 delay 5 seconds @00:41:34.27 Running ENTem ProfileEM60C thermocycling @00:41:34.28 PCR.program ProfileEM60C @00:41:34.39 ProfileEM60C should complete in < 2:11:38 @00:41:34.40 PCR.run 2:49:32.5 @02:41:13.20 PCR.off @02:41:13.74 PCR for ENTem completed -- closed /var/log/esp/ENTem14feb1200h1000ml.pcr @02:41:13.79 Cleaning PCR @02:41:13.80 AFV.select :carrierSolution @02:41:13.89 delay 2 seconds @02:41:15.90 AS.reconfigure ASconfig @02:41:15.93 AS.seek 0.500ml @02:41:26.36 delay 1 second @02:41:27.37 AFV.select :holdingCoil @02:41:27.46 ASV.dial :waste1 @02:41:27.92 ATV.dial :air3 @02:41:28.94 AS.reconfigure ASslow1 @02:41:28.97 AS.seek 0.510ml @02:41:31.28 delay 2 seconds @02:41:33.29 ATV.dial :bleach @02:41:33.66 AS.seek 0.810ml @02:42:35.11 delay 3 seconds @02:42:38.12 ATV.dial :air3 @02:42:38.45 delay 3 seconds @02:42:41.51 AS.seek 0.835ml @02:42:46.84 delay 3 seconds @02:42:49.85 ATV.dial :bleach @02:42:50.22 AS.seek 0.820ml @02:42:53.55 delay 3 seconds @02:42:56.56 ARV.dial :waste @02:42:57.15 delay 3 seconds @02:43:00.16 ATV.dial :ARV @02:43:00.89 AS.reconfigure ASconfig @02:43:00.92 AS.seek 0.470ml @02:43:21.61 AFV.select :carrierSolution @02:43:21.71 delay 2 seconds @02:43:23.72 AS.seek 1.000ml @02:43:54.87 delay 1 second @02:43:55.88 AFV.select :holdingCoil @02:43:55.98 delay 1 second @02:43:56.99 AS.reconfigure ASfast @02:43:57.02 AS.seek 0.000ml @02:44:24.76 AFV.select :carrierSolution @02:44:24.86 delay 2 seconds @02:44:26.87 AS.reconfigure ASconfig @02:44:26.90 AS.seek 1.000ml @02:45:25.50 delay 1 second @02:45:26.51 AFV.select :holdingCoil @02:45:26.60 delay 1 second @02:45:27.61 AS.reconfigure ASfast @02:45:27.64 AS.seek 0.000ml @02:45:55.33 AFV.select :carrierSolution @02:45:55.43 delay 2 seconds @02:45:57.44 AS.reconfigure ASconfig @02:45:57.47 AS.seek 1.000ml @02:46:56.06 delay 1 second @02:46:57.07 AFV.select :holdingCoil @02:46:57.16 delay 1 second @02:46:58.17 AS.reconfigure ASfast @02:46:58.20 AS.seek 0.000ml @02:47:25.94 ARV.dial between carrier and air1 @02:47:26.36 ATV.dial :air3 @02:47:27.27 AS.reconfigure ASslow1 @02:47:27.31 AS.seek 0.010ml @02:47:29.67 delay 2 seconds @02:47:31.68 ATV.dial :bleach @02:47:32.05 AS.seek 0.810ml @02:50:15.51 delay 3 seconds @02:50:18.52 ATV.dial :air3 @02:50:18.85 delay 3 seconds @02:50:21.91 AS.seek 0.835ml @02:50:27.26 delay 3 seconds @02:50:30.27 ATV.dial :bleach @02:50:30.65 AS.seek 0.820ml @02:50:33.98 delay 3 seconds @02:50:36.99 ATV.dial PCR @02:50:37.42 AS.reconfigure ASconfig @02:50:37.45 AS.seek empty @02:51:25.54 PCR.program :bleachProfile @02:51:25.60 PCR program should complete in < 2:45 @02:51:25.61 PCR.run 10:00 @02:54:21.31 PCR.off @02:54:21.34 AFV.select :carrierSolution @02:54:21.43 delay 2 seconds @02:54:23.44 AS.seek 1.000ml @02:55:22.08 delay 1 second @02:55:23.09 AFV.select :holdingCoil @02:55:23.18 delay 1 second @02:55:24.19 AS.reconfigure ASfast @02:55:24.23 AS.seek 0.000ml @02:55:51.97 delay 3 seconds @02:55:54.98 AFV.select :carrierSolution @02:55:55.07 delay 2 seconds @02:55:57.08 AS.reconfigure ASconfig @02:55:57.11 AS.seek 1.000ml @02:56:55.73 delay 1 second @02:56:56.74 AFV.select :holdingCoil @02:56:56.83 delay 1 second @02:56:57.84 AS.reconfigure ASfast @02:56:57.88 AS.seek 0.000ml @02:57:25.57 delay 3 seconds @02:57:28.58 AFV.select :carrierSolution @02:57:28.67 delay 2 seconds @02:57:30.68 AS.reconfigure ASconfig @02:57:30.71 AS.seek 1.000ml @02:58:29.29 delay 1 second @02:58:30.30 AFV.select :holdingCoil @02:58:30.39 delay 1 second @02:58:31.40 AS.reconfigure ASfast @02:58:31.43 AS.seek 0.000ml @02:58:59.18 delay 3 seconds @02:59:02.19 AFV.select :carrierSolution @02:59:02.28 delay 2 seconds @02:59:04.29 AS.reconfigure ASconfig @02:59:04.32 AS.seek 1.000ml @03:00:02.93 delay 1 second @03:00:03.94 AFV.select :holdingCoil @03:00:04.03 delay 1 second @03:00:05.04 AS.reconfigure ASfast @03:00:05.07 AS.seek 0.000ml @03:00:32.80 delay 3 seconds @03:00:35.81 AFV.select :carrierSolution @03:00:35.90 delay 2 seconds @03:00:37.91 AS.reconfigure ASconfig @03:00:37.94 AS.seek 1.000ml @03:01:36.56 delay 1 second @03:01:37.57 AFV.select :holdingCoil @03:01:37.66 delay 1 second @03:01:38.67 AS.reconfigure ASnorm @03:01:38.70 AS.seek 0.000ml @03:02:30.16 Setting up PCR for IPCap -- writing /var/log/esp/IPCap14feb1203h1000ml.pcr @03:02:30.17 Building PCR Slug from ASV with MM1 mix, PP2 primer @03:02:30.18 Priming with carrier @03:02:30.19 ATV.dial ARV @03:02:30.70 ARV.dial :carrier @03:02:31.00 AS.seek 0.500ml @03:02:56.88 AFV.select :carrierSolution @03:02:56.98 delay 2 seconds @03:02:58.99 AS.reconfigure ASconfig @03:02:59.03 AS.seek 1.000ml @03:03:28.46 delay 1 second @03:03:29.47 AFV.select :holdingCoil @03:03:29.56 delay 1 second @03:03:30.57 ATV.dial :waste @03:03:31.81 AS.reconfigure ASnorm @03:03:31.84 AS.seek empty @03:04:23.32 AFV.select :carrierSolution @03:04:23.42 delay 2 seconds @03:04:25.43 AS.reconfigure ASconfig @03:04:25.47 AS.seek 0.500ml @03:04:54.96 delay 1 second @03:04:55.97 AFV.select :holdingCoil @03:04:56.06 Building Slug @03:04:56.07 ATV.dial :ARV @03:04:57.30 delay 2 seconds @03:04:59.31 ARV.dial :air1 @03:04:59.64 delay 2 seconds @03:05:01.70 AS.reconfigure ASslow2 @03:05:01.73 AS.seek 0.506ml @03:05:08.00 delay 2 seconds @03:05:10.01 ARV.dial :MM1 @03:05:10.49 delay 2 seconds @03:05:12.50 Adding MM1 @03:05:12.55 AS.seek 0.524ml @03:05:31.46 delay 25 seconds @03:05:56.47 ARV.dial :PP2 @03:05:56.80 Adding PP2 @03:05:56.81 delay 2 seconds @03:05:58.87 AS.seek 0.530ml @03:06:05.31 delay 10 seconds @03:06:15.32 ARV.dial :air2 @03:06:15.74 adding ARV air @03:06:15.75 delay 2 seconds @03:06:17.81 AS.seek 0.536ml @03:06:24.31 delay 5 seconds @03:06:29.32 ARV.dial :carrier @03:06:30.33 delay 7 seconds @03:06:37.39 AS.seek 0.594ml @03:07:36.86 delay 5 seconds @03:07:41.87 ATV.dial :ASV @03:07:42.19 delay 2 seconds @03:07:44.25 AS.seek 0.600ml @03:07:50.81 delay 4 seconds @03:07:54.82 ATV.dial ARV @03:07:55.15 delay 2 seconds @03:07:57.16 ARV.dial :carrier @03:07:57.17 delay 2 seconds @03:07:59.22 AS.seek 0.650ml @03:08:50.39 delay 4 seconds @03:08:54.40 ATV.dial PCR @03:08:54.89 ASV.dial :waste1 @03:08:54.90 ARV.dial :air1 @03:08:55.24 Positioning Slug by 0.324ml @03:08:55.29 AS.seek 0.326ml @03:14:26.19 delay 5 seconds @03:14:31.20 ATV.dial ARV @03:14:31.80 ASV.dial :PCRlock @03:14:32.55 delay 5 seconds @03:14:37.56 Running IPCap Profile59C thermocycling @03:14:37.57 PCR.program Profile59C @03:14:37.68 Profile59C should complete in < 1:39:08 @03:14:37.69 PCR.run 2:08:55 @04:42:09.79 PCR.off @04:42:10.33 PCR for IPCap completed -- closed /var/log/esp/IPCap14feb1203h1000ml.pcr @04:42:10.38 Cleaning PCR @04:42:10.39 AFV.select :carrierSolution @04:42:10.48 delay 2 seconds @04:42:12.49 AS.reconfigure ASconfig @04:42:12.52 AS.seek 0.500ml @04:42:22.96 delay 1 second @04:42:23.97 AFV.select :holdingCoil @04:42:24.06 ASV.dial :waste1 @04:42:24.38 ATV.dial :air3 @04:42:25.30 AS.reconfigure ASslow1 @04:42:25.34 AS.seek 0.510ml @04:42:27.63 delay 2 seconds @04:42:29.64 ATV.dial :bleach @04:42:30.01 AS.seek 0.810ml @04:43:31.47 delay 3 seconds @04:43:34.48 ATV.dial :air3 @04:43:34.91 delay 3 seconds @04:43:37.98 AS.seek 0.835ml @04:43:43.33 delay 3 seconds @04:43:46.34 ATV.dial :bleach @04:43:46.72 AS.seek 0.820ml @04:43:50.03 delay 3 seconds @04:43:53.04 ARV.dial :waste @04:43:53.55 delay 3 seconds @04:43:56.56 ATV.dial :ARV @04:43:57.29 AS.reconfigure ASconfig @04:43:57.33 AS.seek 0.470ml @04:44:18.00 AFV.select :carrierSolution @04:44:18.09 delay 2 seconds @04:44:20.10 AS.seek 1.000ml @04:44:51.29 delay 1 second @04:44:52.30 AFV.select :holdingCoil @04:44:52.39 delay 1 second @04:44:53.40 AS.reconfigure ASfast @04:44:53.43 AS.seek 0.000ml @04:45:21.17 AFV.select :carrierSolution @04:45:21.27 delay 2 seconds @04:45:23.28 AS.reconfigure ASconfig @04:45:23.31 AS.seek 1.000ml @04:46:21.93 delay 1 second @04:46:22.94 AFV.select :holdingCoil @04:46:23.03 delay 1 second @04:46:24.04 AS.reconfigure ASfast @04:46:24.07 AS.seek 0.000ml @04:46:51.82 AFV.select :carrierSolution @04:46:51.92 delay 2 seconds @04:46:53.93 AS.reconfigure ASconfig @04:46:53.96 AS.seek 1.000ml @04:47:52.57 delay 1 second @04:47:53.58 AFV.select :holdingCoil @04:47:53.67 delay 1 second @04:47:54.68 AS.reconfigure ASfast @04:47:54.71 AS.seek 0.000ml @04:48:23.00 ARV.dial between carrier and air1 @04:48:23.41 ATV.dial :air3 @04:48:24.34 AS.reconfigure ASslow1 @04:48:24.37 AS.seek 0.010ml @04:48:26.71 delay 2 seconds @04:48:28.72 ATV.dial :bleach @04:48:29.10 AS.seek 0.810ml @04:51:12.56 delay 3 seconds @04:51:15.57 ATV.dial :air3 @04:51:15.89 delay 3 seconds @04:51:18.95 AS.seek 0.835ml @04:51:24.29 delay 3 seconds @04:51:27.30 ATV.dial :bleach @04:51:27.67 AS.seek 0.820ml @04:51:31.00 delay 3 seconds @04:51:34.01 ATV.dial PCR @04:51:34.35 AS.reconfigure ASconfig @04:51:34.38 AS.seek empty @04:52:22.51 PCR.program :bleachProfile @04:52:22.57 PCR program should complete in < 2:45 @04:52:22.58 PCR.run 10:00 @04:55:17.75 PCR.off @04:55:17.78 AFV.select :carrierSolution @04:55:17.87 delay 2 seconds @04:55:19.88 AS.seek 1.000ml @04:56:18.53 delay 1 second @04:56:19.54 AFV.select :holdingCoil @04:56:19.63 delay 1 second @04:56:20.64 AS.reconfigure ASfast @04:56:20.67 AS.seek 0.000ml @04:56:48.41 delay 3 seconds @04:56:51.42 AFV.select :carrierSolution @04:56:51.51 delay 2 seconds @04:56:53.52 AS.reconfigure ASconfig @04:56:53.55 AS.seek 1.000ml @04:57:52.14 delay 1 second @04:57:53.15 AFV.select :holdingCoil @04:57:53.24 delay 1 second @04:57:54.25 AS.reconfigure ASfast @04:57:54.28 AS.seek 0.000ml @04:58:22.01 delay 3 seconds @04:58:25.02 AFV.select :carrierSolution @04:58:25.11 delay 2 seconds @04:58:27.12 AS.reconfigure ASconfig @04:58:27.15 AS.seek 1.000ml @04:59:25.75 delay 1 second @04:59:26.76 AFV.select :holdingCoil @04:59:26.85 delay 1 second @04:59:27.86 AS.reconfigure ASfast @04:59:27.90 AS.seek 0.000ml @04:59:55.64 delay 3 seconds @04:59:58.65 AFV.select :carrierSolution @04:59:58.74 delay 2 seconds @05:00:00.75 AS.reconfigure ASconfig @05:00:00.78 AS.seek 1.000ml @05:00:59.39 delay 1 second @05:01:00.40 AFV.select :holdingCoil @05:01:00.49 delay 1 second @05:01:01.50 AS.reconfigure ASfast @05:01:01.53 AS.seek 0.000ml @05:01:29.27 delay 3 seconds @05:01:32.28 AFV.select :carrierSolution @05:01:32.37 delay 2 seconds @05:01:34.38 AS.reconfigure ASconfig @05:01:34.41 AS.seek 1.000ml @05:02:33.00 delay 1 second @05:02:34.01 AFV.select :holdingCoil @05:02:34.10 delay 1 second @05:02:35.11 AS.reconfigure ASnorm @05:02:35.14 AS.seek 0.000ml @05:03:26.59 Setting up PCR for IPCem -- writing /var/log/esp/IPCem14feb1205h1000ml.pcr @05:03:26.60 Building PCR Slug from ASV with MM3 mix, PP5 primer @05:03:26.61 Priming with carrier ATV.dial ARV @05:03:27.11 ARV.dial :carrier @05:03:27.39 AS.seek 0.500ml @05:03:53.26 AFV.select :carrierSolution @05:03:53.36 delay 2 seconds @05:03:55.37 AS.reconfigure ASconfig @05:03:55.40 AS.seek 1.000ml @05:04:24.84 delay 1 second @05:04:25.85 AFV.select :holdingCoil @05:04:25.94 delay 1 second @05:04:26.95 ATV.dial :waste @05:04:28.19 AS.reconfigure ASnorm @05:04:28.22 AS.seek empty @05:05:19.70 AFV.select :carrierSolution @05:05:19.80 delay 2 seconds @05:05:21.81 AS.reconfigure ASconfig @05:05:21.84 AS.seek 0.500ml @05:05:51.32 delay 1 second @05:05:52.33 AFV.select :holdingCoil @05:05:52.42 Building Slug @05:05:52.43 ATV.dial :ARV @05:05:53.67 delay 2 seconds @05:05:55.68 ARV.dial :air1 @05:05:56.01 delay 2 seconds @05:05:58.07 AS.reconfigure ASslow2 @05:05:58.10 AS.seek 0.506ml @05:06:04.38 delay 2 seconds @05:06:06.39 ARV.dial :MM3 @05:06:07.24 delay 2 seconds @05:06:09.25 Adding MM3 @05:06:09.30 AS.seek 0.524ml @05:06:27.95 delay 25 seconds @05:06:52.96 ARV.dial :PP5 @05:06:53.27 Adding PP5 @05:06:53.28 delay 2 seconds @05:06:55.34 AS.seek 0.530ml @05:07:01.98 delay 10 seconds @05:07:11.99 ARV.dial :air3 @05:07:12.41 adding ARV air @05:07:12.42 delay 2 seconds @05:07:14.48 AS.seek 0.536ml @05:07:20.77 delay 5 seconds @05:07:25.78 ARV.dial :carrier @05:07:26.79 delay 7 seconds @05:07:33.85 AS.seek 0.594ml @05:08:33.72 delay 5 seconds @05:08:38.73 ATV.dial :ASV @05:08:39.03 delay 2 seconds @05:08:41.09 AS.seek 0.600ml @05:08:48.19 delay 4 seconds @05:08:52.20 ATV.dial ARV @05:08:52.53 delay 2 seconds @05:08:54.54 ARV.dial :carrier @05:08:54.55 delay 2 seconds @05:08:56.60 AS.seek 0.650ml @05:09:47.97 delay 4 seconds @05:09:51.98 ATV.dial PCR @05:09:52.47 ASV.dial :waste1 @05:09:52.48 ARV.dial :air1 @05:09:52.90 Positioning Slug by 0.324ml @05:09:52.96 AS.seek 0.326ml @05:15:23.83 delay 5 seconds @05:15:28.84 ATV.dial ARV @05:15:29.33 ASV.dial :PCRlock @05:15:30.04 delay 5 seconds @05:15:35.05 Running IPCem ProfileEM59C thermocycling @05:15:35.06 PCR.program ProfileEM59C @05:15:35.17 ProfileEM59C should complete in < 2:11:38 @05:15:35.18 PCR.run 2:49:32.5 @07:16:04.05 PCR.off @07:16:04.58 PCR for IPCem completed -- closed /var/log/esp/IPCem14feb1205h1000ml.pcr @07:16:04.59 Full MFB Decontamination @07:16:04.60 Decon extraction column and decon AIV @07:16:04.62 SPE.hold 2:46:40,55.0C @07:16:04.63 ASV.dial :SPEdelivery @07:16:05.20 delay 20 seconds @07:16:25.21 AFV.select :carrierSolution @07:16:25.30 delay 2 seconds @07:16:27.31 AS.reconfigure ASconfig @07:16:27.34 AS.seek 0.500ml @07:16:37.75 delay 1 second @07:16:38.76 AFV.select :holdingCoil @07:16:38.85 ATV.dial :air1 @07:16:40.48 AS.reconfigure ASslow1 @07:16:40.51 AS.seek 0.520ml @07:16:44.85 ATV.dial :ASV @07:16:46.75 ASV.dial :SPErecovery @07:16:47.30 AS.seek 0.620ml @07:17:07.94 ATV.dial :waste @07:17:09.50 AS.reconfigure ASnorm @07:17:09.53 AS.seek empty @07:17:41.60 AFV.select :holdingCoil @07:17:41.61 ATV.dial :waste @07:17:41.62 AS.reconfigure ASconfig @07:17:41.66 AS.seek 0.000ml @07:17:41.95 AFV.select :carrierSolution @07:17:42.05 delay 2 seconds @07:17:44.06 AS.seek 0.500ml @07:18:13.47 delay 1 second @07:18:14.48 AFV.select :holdingCoil @07:18:14.57 ATV.dial :air3 @07:18:15.14 AS.reconfigure ASslow1 @07:18:15.17 AS.seek 0.520ml @07:18:19.53 ATV.dial :air3 @07:18:19.59 AS.seek 0.530ml @07:18:21.88 delay 2 seconds @07:18:23.89 ATV.dial :bleach @07:18:24.25 AS.seek 0.710ml @07:19:01.22 delay 3 seconds @07:19:04.23 ATV.dial :air3 @07:19:04.56 delay 3 seconds @07:19:07.62 AS.seek 0.735ml @07:19:12.98 delay 3 seconds @07:19:15.99 ATV.dial :bleach @07:19:16.36 AS.seek 0.720ml @07:19:19.67 delay 3 seconds @07:19:22.68 ATV.dial ASV @07:19:23.56 ASV.dial :SPErecovery @07:19:23.62 AS.seek 0.365ml @07:20:36.28 ATV.dial :waste @07:20:37.71 AS.reconfigure ASnorm @07:20:37.74 AS.seek empty @07:20:56.78 ATV.dial :air3 @07:20:57.44 AS.reconfigure ASslow1 @07:20:57.47 AS.seek 0.009ml @07:20:59.82 delay 2 seconds @07:21:01.83 ATV.dial :bleach @07:21:02.20 AS.seek 0.579ml @07:22:58.68 delay 3 seconds @07:23:01.69 ATV.dial :air3 @07:23:02.02 delay 3 seconds @07:23:05.08 AS.seek 0.604ml @07:23:10.44 delay 3 seconds @07:23:13.45 ATV.dial :bleach @07:23:13.82 AS.seek 0.589ml @07:23:17.15 delay 3 seconds @07:23:20.16 ATV.dial :ASV @07:23:21.04 ASV.dial :wasteR @07:23:21.53 AS.seek 0.000ml @07:25:22.06 delay 1:00 @07:26:22.07 ATV.dial ASV @07:26:22.08 AFV.select :carrierSolution @07:26:22.17 delay 2 seconds @07:26:24.18 AS.reconfigure ASconfig @07:26:24.21 AS.seek 1.000ml @07:27:22.78 delay 1 second @07:27:23.79 AFV.select :holdingCoil @07:27:23.88 delay 1 second @07:27:24.89 ASV.dial :wasteR @07:27:24.90 AS.reconfigure ASslow1 @07:27:24.93 AS.seek 0.000ml @07:30:49.32 delay 10 seconds @07:30:59.33 ATV.dial :air3 @07:31:00.45 AS.seek 0.010ml @07:31:02.74 delay 2 seconds @07:31:04.75 ATV.dial :bleach @07:31:05.13 AS.seek 0.580ml @07:33:01.66 delay 3 seconds @07:33:04.67 ATV.dial :air3 @07:33:05.00 delay 3 seconds @07:33:08.06 AS.seek 0.605ml @07:33:13.39 delay 3 seconds @07:33:16.40 ATV.dial :bleach @07:33:16.77 AS.seek 0.590ml @07:33:20.09 delay 3 seconds @07:33:23.10 ATV.dial ASV @07:33:24.06 ASV.dial :SPEdelivery @07:33:24.39 AS.seek 0.000ml @07:35:24.96 delay 1:00 @07:36:24.97 AFV.select :carrierSolution @07:36:25.06 delay 2 seconds @07:36:27.07 AS.reconfigure ASconfig @07:36:27.10 AS.seek 1.000ml @07:37:25.70 delay 1 second @07:37:26.71 AFV.select :holdingCoil @07:37:26.80 delay 1 second @07:37:27.81 ATV.dial ASV @07:37:27.82 ASV.dial :SPEdelivery @07:37:27.83 AS.reconfigure ASslow1 @07:37:27.86 AS.seek 0.000ml @07:40:52.09 delay 10 seconds @07:41:02.10 ATV.dial :air2 @07:41:02.93 AS.reconfigure ASslow0 @07:41:02.96 AS.seek 0.800ml @07:42:24.90 delay 3 seconds @07:42:27.91 ATV.dial :ASV @07:42:29.48 ASV.dial :SPEdelivery @07:42:29.50 AS.reconfigure ASslow1 @07:42:29.53 AS.seek 0.030ml @07:45:06.84 delay 20 seconds @07:45:26.85 ATV.dial :waste,via => air3 @07:45:28.28 AS.reconfigure ASconfig @07:45:28.31 AS.seek empty @07:45:30.41 SPE.coast @07:45:30.49 Removing Bleach from Mixing Coil @07:45:30.50 AFV.select :carrierSolution @07:45:30.59 delay 2 seconds @07:45:32.60 AS.seek 0.500ml @07:46:02.09 delay 1 second @07:46:03.10 AFV.select :holdingCoil @07:46:03.19 ATV.dial :ASV @07:46:04.61 ASV.dial :SPErecovery @07:46:05.29 AS.reconfigure ASslow1 @07:46:05.33 AS.seek 0.730ml @07:46:52.50 ASV.dial :wasteD @07:46:53.01 AS.reconfigure ASnorm @07:46:53.04 AS.seek 0.000ml @07:47:31.20 AFV.select :carrierSolution @07:47:31.30 delay 2 seconds @07:47:33.31 AS.reconfigure ASconfig @07:47:33.34 AS.seek 0.500ml @07:48:02.78 delay 1 second @07:48:03.79 AFV.select :holdingCoil @07:48:03.88 ATV.dial ASV @07:48:03.89 ASV.dial :SPErecovery @07:48:04.86 AS.reconfigure ASslow0 @07:48:04.89 AS.seek 0.300ml @07:48:25.56 ATV.dial :air2 @07:48:26.42 AS.reconfigure ASslow1 @07:48:26.45 AS.seek 0.320ml @07:48:30.78 ATV.dial ASV @07:48:31.53 AS.reconfigure ASslow0 @07:48:31.56 AS.seek 0.570ml @07:48:57.37 ASV.dial :wasteD @07:48:57.86 AS.reconfigure ASnorm @07:48:57.89 AS.seek 0.000ml @07:49:27.35 AFV.select :carrierSolution @07:49:27.45 delay 2 seconds @07:49:29.46 AS.reconfigure ASconfig @07:49:29.49 AS.seek 0.500ml @07:49:58.90 delay 1 second @07:49:59.91 AFV.select :holdingCoil @07:50:00.00 ATV.dial ASV @07:50:00.01 ASV.dial :SPErecovery @07:50:00.98 AS.reconfigure ASslow0 @07:50:01.01 AS.seek 0.300ml @07:50:21.71 ATV.dial :air2 @07:50:22.46 AS.reconfigure ASslow1 @07:50:22.49 AS.seek 0.320ml @07:50:26.85 ATV.dial ASV @07:50:27.60 AS.reconfigure ASslow0 @07:50:27.63 AS.seek 0.570ml @07:50:53.42 ASV.dial :wasteD @07:50:53.92 AS.reconfigure ASnorm @07:50:53.95 AS.seek 0.000ml @07:51:23.40 ATV.dial :air2 @07:51:24.12 AS.reconfigure ASslow0 @07:51:24.15 AS.seek 0.300ml @07:51:55.06 ATV.dial :ASV @07:51:55.76 ASV.dial :wasteD @07:51:55.78 AS.reconfigure ASconfig @07:51:55.81 AS.seek 0.050ml @07:52:10.65 ATV.dial :waste @07:52:12.07 AS.seek 0.000ml @07:52:15.26 ASV.dial :SPEdelivery @07:52:16.40 deconLysateINT @07:52:16.41 ATV.dial :air3 @07:52:17.05 AS.reconfigure ASslow1 @07:52:17.09 AS.seek 0.010ml @07:52:19.42 delay 2 seconds @07:52:21.43 ATV.dial :bleach @07:52:21.81 AS.seek 0.960ml @07:55:35.79 delay 3 seconds @07:55:38.80 ATV.dial :air3 @07:55:39.15 delay 3 seconds @07:55:42.21 AS.seek 0.985ml @07:55:47.55 delay 3 seconds @07:55:50.56 ATV.dial :bleach @07:55:51.04 AS.seek 0.970ml @07:55:54.38 delay 3 seconds @07:55:57.39 ATV.dial :AIV @07:55:58.46 AIV.dial :coilToMFB @07:55:58.47 AWV.select :waste @07:55:58.48 AFV.select :carrierSolution @07:55:58.57 delay 2 seconds @07:56:00.58 AS.reconfigure ASconfig @07:56:00.61 AS.seek 1.000ml @07:56:02.69 delay 1 second @07:56:03.70 AFV.select :holdingCoil @07:56:03.79 delay 1 second @07:56:04.80 AS.reconfigure ASslow0 @07:56:04.83 AS.seek empty @07:57:47.23 AFV.select :carrierSolution @07:57:47.33 delay 2 seconds @07:57:49.34 AS.reconfigure ASconfig @07:57:49.37 AS.seek 1.000ml @07:58:48.03 delay 1 second @07:58:49.04 AFV.select :holdingCoil @07:58:49.13 delay 1 second @07:58:50.14 AS.reconfigure ASslow0 @07:58:50.17 AS.seek empty @08:00:32.57 AFV.select :carrierSolution @08:00:32.67 delay 2 seconds @08:00:34.68 AS.reconfigure ASconfig @08:00:34.71 AS.seek 1.000ml @08:01:33.35 delay 1 second @08:01:34.36 AFV.select :holdingCoil @08:01:34.45 delay 1 second @08:01:35.46 AS.reconfigure ASslow0 @08:01:35.49 AS.seek empty @08:03:17.90 ATV.dial :air2 @08:03:18.48 AS.reconfigure ASslow1 @08:03:18.51 AS.seek 0.009ml @08:03:20.88 AS.reconfigure ASslow0 @08:03:20.92 AS.seek 1.000ml @08:05:02.20 ATV.dial AIV @08:05:02.73 AS.seek 0.020ml @08:06:43.04 ATV.dial :waste @08:06:44.38 AS.seek 0.000ml @08:06:46.68 AWV.select :air @08:06:46.83 Cleaning PCR @08:06:46.84 AFV.select :carrierSolution @08:06:46.93 delay 2 seconds @08:06:48.94 AS.reconfigure ASconfig @08:06:48.97 AS.seek 0.500ml @08:07:18.39 delay 1 second @08:07:19.40 AFV.select :holdingCoil @08:07:19.49 ASV.dial :waste1 @08:07:19.87 ATV.dial :air3 @08:07:20.51 AS.reconfigure ASslow1 @08:07:20.54 AS.seek 0.510ml @08:07:22.86 delay 2 seconds @08:07:24.87 ATV.dial :bleach @08:07:25.25 AS.seek 0.810ml @08:08:26.71 delay 3 seconds @08:08:29.72 ATV.dial :air3 @08:08:30.05 delay 3 seconds @08:08:33.11 AS.seek 0.835ml @08:08:38.97 delay 3 seconds @08:08:41.98 ATV.dial :bleach @08:08:42.35 AS.seek 0.820ml @08:08:45.67 delay 3 seconds @08:08:48.68 ARV.dial :waste @08:08:49.17 delay 3 seconds @08:08:52.18 ATV.dial :ARV @08:08:52.92 AS.reconfigure ASconfig @08:08:52.95 AS.seek 0.470ml @08:09:13.64 AFV.select :carrierSolution @08:09:13.74 delay 2 seconds @08:09:15.75 AS.seek 1.000ml @08:09:46.99 delay 1 second @08:09:48.00 AFV.select :holdingCoil @08:09:48.09 delay 1 second @08:09:49.10 AS.reconfigure ASfast @08:09:49.13 AS.seek 0.000ml @08:10:16.87 AFV.select :carrierSolution @08:10:16.97 delay 2 seconds @08:10:18.98 AS.reconfigure ASconfig @08:10:19.01 AS.seek 1.000ml @08:11:17.56 delay 1 second @08:11:18.57 AFV.select :holdingCoil @08:11:18.66 delay 1 second @08:11:19.67 AS.reconfigure ASfast @08:11:19.70 AS.seek 0.000ml @08:11:47.43 AFV.select :carrierSolution @08:11:47.53 delay 2 seconds @08:11:49.54 AS.reconfigure ASconfig @08:11:49.57 AS.seek 1.000ml @08:12:48.18 delay 1 second @08:12:49.19 AFV.select :holdingCoil @08:12:49.28 delay 1 second @08:12:50.29 AS.reconfigure ASfast @08:12:50.32 AS.seek 0.000ml @08:13:18.07 ARV.dial between carrier and air1 @08:13:18.48 ATV.dial :air3 @08:13:19.42 AS.reconfigure ASslow1 @08:13:19.45 AS.seek 0.010ml @08:13:21.79 delay 2 seconds @08:13:23.80 ATV.dial :bleach @08:13:24.18 AS.seek 0.810ml @08:16:07.53 delay 3 seconds @08:16:10.54 ATV.dial :air3 @08:16:10.97 delay 3 seconds @08:16:14.03 AS.seek 0.835ml @08:16:19.36 delay 3 seconds @08:16:22.37 ATV.dial :bleach @08:16:22.83 AS.seek 0.820ml @08:16:26.19 delay 3 seconds @08:16:29.20 ATV.dial PCR @08:16:29.54 AS.reconfigure ASconfig @08:16:29.57 AS.seek empty @08:17:17.69 PCR.program :bleachProfile @08:17:17.75 PCR program should complete in < 2:45 @08:17:17.76 PCR.run 10:00 @08:20:18.97 PCR.off @08:20:19.00 AFV.select :carrierSolution @08:20:19.09 delay 2 seconds @08:20:21.10 AS.seek 1.000ml @08:21:19.76 delay 1 second @08:21:20.77 AFV.select :holdingCoil @08:21:20.86 delay 1 second @08:21:21.87 AS.reconfigure ASfast @08:21:21.90 AS.seek 0.000ml @08:21:49.59 delay 3 seconds @08:21:52.60 AFV.select :carrierSolution @08:21:52.69 delay 2 seconds @08:21:54.70 AS.reconfigure ASconfig @08:21:54.73 AS.seek 1.000ml @08:22:53.32 delay 1 second @08:22:54.33 AFV.select :holdingCoil @08:22:54.42 delay 1 second @08:22:55.43 AS.reconfigure ASfast @08:22:55.46 AS.seek 0.000ml @08:23:23.19 delay 3 seconds @08:23:26.20 AFV.select :carrierSolution @08:23:26.29 delay 2 seconds @08:23:28.30 AS.reconfigure ASconfig @08:23:28.33 AS.seek 1.000ml @08:24:26.94 delay 1 second @08:24:27.95 AFV.select :holdingCoil @08:24:28.04 delay 1 second @08:24:29.05 AS.reconfigure ASfast @08:24:29.08 AS.seek 0.000ml @08:24:56.82 delay 3 seconds @08:24:59.83 AFV.select :carrierSolution @08:24:59.92 delay 2 seconds @08:25:01.93 AS.reconfigure ASconfig @08:25:01.96 AS.seek 1.000ml @08:26:00.56 delay 1 second @08:26:01.57 AFV.select :holdingCoil @08:26:01.66 delay 1 second @08:26:02.67 AS.reconfigure ASfast @08:26:02.70 AS.seek 0.000ml @08:26:30.44 delay 3 seconds @08:26:33.45 AFV.select :carrierSolution @08:26:33.54 delay 2 seconds @08:26:35.55 AS.reconfigure ASconfig @08:26:35.59 AS.seek 1.000ml @08:27:34.19 delay 1 second @08:27:35.20 AFV.select :holdingCoil @08:27:35.29 delay 1 second @08:27:36.30 AS.reconfigure ASnorm @08:27:36.33 AS.seek 0.000ml @08:28:27.77 AFV.select :carrierSolution @08:28:27.86 delay 2 seconds @08:28:29.87 AS.reconfigure ASconfig @08:28:29.90 AS.seek 1.000ml @08:29:28.50 delay 1 second @08:29:29.51 AFV.select :holdingCoil @08:29:29.60 delay 1 second @08:29:30.61 ATV.dial :waste @08:29:31.50 AS.reconfigure ASnorm @08:29:31.53 AS.seek 0.500ml @08:29:57.45 ATV.dial :waste2 @08:29:58.53 AS.seek 0.000ml @08:30:24.44
Cleaning PCR AFV.select :carrierSolution @08:30:24.53 delay 2 seconds @08:30:26.54 AS.reconfigure ASconfig @08:30:26.57 AS.seek 0.500ml @08:30:56.01 delay 1 second @08:30:57.02 AFV.select :holdingCoil @08:30:57.11 ASV.dial :waste1 @08:30:57.12 ATV.dial :air3 @08:30:58.57 AS.reconfigure ASslow1 @08:30:58.61 AS.seek 0.510ml @08:31:00.92 delay 2 seconds @08:31:02.93 ATV.dial :bleach @08:31:03.77 AS.seek 0.810ml @08:32:05.21 delay 3 seconds @08:32:08.22 ATV.dial :air3 @08:32:08.54 delay 3 seconds @08:32:11.60 AS.seek 0.835ml @08:32:16.95 delay 3 seconds @08:32:19.96 ATV.dial :bleach @08:32:20.34 AS.seek 0.820ml @08:32:23.67 delay 3 seconds @08:32:26.68 ARV.dial :waste @08:32:27.09 delay 3 seconds @08:32:30.10 ATV.dial :ARV @08:32:30.94 AS.reconfigure ASconfig @08:32:30.97 AS.seek 0.470ml @08:32:51.67 AFV.select :carrierSolution @08:32:51.76 delay 2 seconds @08:32:53.77 AS.seek 1.000ml @08:33:25.01 delay 1 second @08:33:26.03 AFV.select :holdingCoil @08:33:26.12 delay 1 second @08:33:27.13 AS.reconfigure ASfast @08:33:27.16 AS.seek 0.000ml @08:33:54.91 AFV.select :carrierSolution @08:33:55.00 delay 2 seconds @08:33:57.01 AS.reconfigure ASconfig @08:33:57.04 AS.seek 1.000ml @08:34:55.63 delay 1 second @08:34:56.65 AFV.select :holdingCoil @08:34:56.74 delay 1 second @08:34:57.75 AS.reconfigure ASfast @08:34:57.78 AS.seek 0.000ml @08:35:25.52 AFV.select :carrierSolution @08:35:25.61 delay 2 seconds @08:35:27.62 AS.reconfigure ASconfig @08:35:27.65 AS.seek 1.000ml @08:36:26.27 delay 1 second @08:36:27.28 AFV.select :holdingCoil @08:36:27.37 delay 1 second @08:36:28.38 AS.reconfigure ASfast @08:36:28.42 AS.seek 0.000ml @08:36:56.16 ARV.dial between carrier and air1 @08:36:56.56 ATV.dial :air3 @08:36:57.47 AS.reconfigure ASslow1 @08:36:57.50 AS.seek 0.010ml @08:36:59.84 delay 2 seconds @08:37:01.85 ATV.dial :bleach @08:37:02.22 AS.seek 0.810ml @08:39:45.67 delay 3 seconds @08:39:48.68 ATV.dial :air3 @08:39:49.01 delay 3 seconds @08:39:52.07 AS.seek 0.835ml @08:39:57.46 delay 3 seconds @08:40:00.47 ATV.dial :bleach @08:40:00.84 AS.seek 0.820ml @08:40:04.15 delay 3 seconds @08:40:07.16 ATV.dial PCR @08:40:07.49 AS.reconfigure ASconfig @08:40:07.52 AS.seek empty @08:40:55.65 PCR.program :bleachProfile @08:40:55.71 PCR program should complete in < 2:45 @08:40:55.72 PCR.run 10:00 @08:43:53.92 PCR.off @08:43:53.95 AFV.select :carrierSolution @08:43:54.04 delay 2 seconds @08:43:56.05 AS.seek 1.000ml @08:44:54.73 delay 1 second @08:44:55.74 AFV.select :holdingCoil @08:44:55.83 delay 1 second @08:44:56.84 AS.reconfigure ASfast @08:44:56.87 AS.seek 0.000ml @08:45:24.60 delay 3 seconds @08:45:27.61 AFV.select :carrierSolution @08:45:27.70 delay 2 seconds @08:45:29.71 AS.reconfigure ASconfig @08:45:29.74 AS.seek 1.000ml @08:46:28.33 delay 1 second @08:46:29.34 AFV.select :holdingCoil @08:46:29.43 delay 1 second @08:46:30.44 AS.reconfigure ASfast @08:46:30.47 AS.seek 0.000ml @08:46:58.21 delay 3 seconds @08:47:01.23 AFV.select :carrierSolution @08:47:01.32 delay 2 seconds @08:47:03.33 AS.reconfigure ASconfig @08:47:03.36 AS.seek 1.000ml @08:48:01.97 delay 1 second @08:48:02.98 AFV.select :holdingCoil @08:48:03.07 delay 1 second @08:48:04.08 AS.reconfigure ASfast @08:48:04.11 AS.seek 0.000ml @08:48:31.80 delay 3 seconds @08:48:34.82 AFV.select :carrierSolution @08:48:34.91 delay 2 seconds @08:48:36.92 AS.reconfigure ASconfig @08:48:36.95 AS.seek 1.000ml @08:49:35.53 delay 1 second @08:49:36.54 AFV.select :holdingCoil @08:49:36.63 delay 1 second @08:49:37.64 AS.reconfigure ASfast @08:49:37.67 AS.seek 0.000ml @08:50:05.42 delay 3 seconds @08:50:08.44 AFV.select :carrierSolution @08:50:08.53 delay 2 seconds @08:50:10.54 AS.reconfigure ASconfig @08:50:10.58 AS.seek 1.000ml @08:51:09.17 delay 1 second @08:51:10.18 AFV.select :holdingCoil @08:51:10.27 delay 1 second @08:51:11.28 AS.reconfigure ASnorm @08:51:11.31 AS.seek 0.000ml @08:52:02.75 Completed {wqPCR} begun 17:40:32 @08:52:02.76 AS.stop @08:52:02.78
Asleep after completing 14feb11SCCWRP assays @08:52:02.79 GoodNews.email "",Subject => "Asleep after completing 14feb11SCCWRP assays" @08:52:02.82 CC.stop @08:52:05.11 CS.stop @08:52:05.14 PC.stop @08:52:05.16 PS.stop @08:52:05.19 Forearm.stop @08:52:05.21 Elbow.stop @08:52:05.24 SS.stop @08:52:05.29 Sleepy.power :raw,:OFF @08:52:05.30 Sleepy.power :dwarf,:OFF @08:52:05.31 Shutdown @08:52:05.32
delayUntil "09:00:00.00" @09:00:00.00 GoodNews.email "neo completed 14feb11SCCWRP mission",Subject => "Ready to Recover" @12:57:54.32PST14-Feb-14 ESP2 v3.20 I2C Gateway v3.01 @12:57:57.47
Configuration for neo by maro@mbari.org /home/esp/esp2/shallow/neo/configure.rb @12:57:57.56 Configuration for MFB10 by preston@mbari.org /home/esp/esp2/mfb/MFB10/configure.rb @12:58:00.76 Sleepy.power :raw,:ON @12:58:01.45 SPE.coast @12:58:01.49 SPE.configure SPEconfig @12:58:01.52 AS.stop @12:58:01.55 AS.configure ASconfig @12:58:01.58 CC.stop @12:58:01.60 CC.configure CCconfig @12:58:01.63 CS.stop @12:58:01.65 CS.configure CSconfig @12:58:01.68 PC.stop @12:58:01.70 PC.configure PCconfig @12:58:01.74 PS.stop @12:58:01.76 PS.configure PSconfig @12:58:01.82 Forearm.stop @12:58:01.84 Forearm.configure LooseForearm @12:58:01.87 Elbow.stop @12:58:01.89 Elbow.configure LooseElbow @12:58:01.95 SC.stop @12:58:01.97 SC.configure SCloose @12:58:02.00 SE.stop @12:58:02.02 SE.configure SEconfig @12:58:02.05 SS.stop @12:58:02.07 SS.configure SSconfig @12:58:02.10 CH.coast @12:58:02.14 CH.configure CHconfig @12:58:02.17 PH.coast @12:58:02.21 PH.configure PHconfig @12:58:02.25 PCR.off @12:58:02.27 PCR.pidtemp= 50,0.1,0,300,25,25 @12:58:02.30 PCR.caltemp= 6.6661,0.0344,0.0 @12:58:02.33 All dwarves are running firmware version 3.74 @12:58:02.89 CTD.latitude = 36.8044 Degrees North in seawater @12:58:02.90 Initializing Sensors @12:58:02.94 GoodNews.email "Starting w/pucks in tube 2\n Ending 13:00PDT26-Mar-14",Subject => "14feb14sccwrp begun" @12:58:03.81
Asleep til +0:10 @12:58:03.86 Sleeping until 12:58 @12:58:03.87 Sleepy.power :raw,:OFF @12:58:03.88 Sleepy.power :dwarf,:OFF @12:58:03.89 Shutdown @12:58:12.68
Powering up Dwarves @12:58:14.75 GoodNews.email "Then, in tube 2: wqPCR\n/home/esp/esp2local/mission/14feb14sccwrp.rb:54",Subject => "Asleep until 12:58" @12:58:17.61
Sleepy.power :raw,:ON @12:58:18.08 SPE.configure SPEconfig @12:58:18.10 AS.configure ASconfig @12:58:18.14 CC.configure CCconfig @12:58:18.17 CS.configure CSconfig @12:58:18.22 PC.configure PCconfig @12:58:18.25 PS.configure PSconfig @12:58:18.28 Forearm.configure LooseForearm @12:58:18.31 Elbow.configure LooseElbow @12:58:18.34 SC.configure SCloose @12:58:18.37 SE.configure SEconfig @12:58:18.40 SS.configure SSconfig @12:58:18.43 CH.configure CHconfig @12:58:18.45 PH.configure PHconfig @12:58:18.48 Valve.reset @12:58:19.12 PCR.off @12:58:19.14 PCR.pidtemp= 50,0.1,0,300,25,25 @12:58:19.17 PCR.caltemp= 6.6661,0.0344,0.0 @12:58:19.28 Intake.select :close @12:58:19.29 Exhaust.select :close @12:58:19.30 CTV.dial :air @12:58:19.34 PSV.dial :air @12:58:19.37
PC.reconfigure PCconfig @12:58:19.45 PC.home.seek open @12:58:19.47 CSV.dial :waste1 @12:58:19.52 PS.reconfigure PSconfig @12:58:19.57 PS.home.seek 0.000ml @12:58:19.59 SSV.dial :spit,avoiding => exhaust @12:58:19.73 CS.reconfigure CSfast @12:58:19.76 SS.reconfigure SSconfig @12:58:19.86 CS.home.seek 0.000ml @12:58:19.89 SS.home.seek 0.00ml @12:58:19.98 CC.reconfigure CCconfig @12:58:20.01 CC.home.seek open @12:58:27.95 Instrument::ReadTimeout in pollContext -- Could not get prompt from CTD on ctd @12:58:27.97 BadNews.email "Could not get prompt from CTD on ctd",Subject => "Instrument::ReadTimeout in pollContext" @12:58:29.88 PS.coast @12:58:29.98
CC.seek open @12:58:30.02 PC.seek open @12:58:30.15
Forearm.reconfigure LooseForearm @12:58:30.18 Forearm.home.seek retract @12:58:30.94 Elbow.jog -1500 @12:58:30.97 Hand.grip @12:58:31.43
Elbow.reconfigure LooseElbow @12:58:31.47 Elbow.home.seek clear @12:58:34.94 SE.reconfigure SEconfig @12:58:34.97 SE.home.seek down @12:58:35.54 SC.reconfigure SCsloppy @12:58:35.58 SC.home.seek initial @12:58:39.24 Forearm.seek retract @12:58:39.30 Elbow.seek hammer @12:58:40.52 Forearm.reconfigure TightForearm @12:58:40.57 Forearm.seek 2 @12:58:41.63 Forearm.seek retract @12:58:42.67 Forearm.reconfigure LooseForearm @12:58:42.97PST14-Feb-14 ===> STARTED wqPCR (phase #1) <=== @12:58:42.98 Starting initialPurge @12:58:42.99 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:58:43.04 Hand.release @12:58:43.89 Elbow.reconfigure TightElbow @12:58:43.92 Elbow.seek FlushPuck::Garage @12:58:45.70 Elbow.reconfigure LooseElbow @12:58:45.79 Forearm.seek garage @12:58:46.82 Hand.grip @12:58:47.64 Forearm.seek retract @12:58:48.66 Elbow.reconfigure TightElbow @12:58:48.70 Elbow.seek CC @12:58:51.55 Elbow.reconfigure LooseElbow @12:58:51.64 Forearm.reconfigure TightForearm @12:58:51.67 Forearm.seek CC @12:58:52.88 CC.seek closed @12:58:56.60 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.96 @12:58:56.65 Hand.release @12:58:57.48 Forearm.reconfigure LooseForearm @12:58:57.51 Forearm.seek retract @12:58:58.46 Flushing the Collection Valve::Manifold @12:58:58.56 CS Pulling 5ml of flush @12:58:58.57 CSV.dial CSR @12:58:59.82 CSR.select :flush @12:58:59.92 CS.reconfigure CSconfig @12:58:59.96 CS.seek 5.000ml @12:59:13.48 delay 5 seconds @12:59:18.49 CSR.select :air @12:59:18.63 CS.reconfigure CSfast @12:59:18.66 CS.seek 7.500ml @12:59:25.17 CSV.dial :waste @12:59:26.64 CS.reconfigure CSconfig @12:59:26.68 CS.seek 0.000ml @12:59:46.42 Purging external Intake @12:59:46.51 CSV.dial :flush @12:59:47.03 CS.reconfigure CSfast @12:59:47.06 CS.seek 10.000ml @13:00:10.45 delay 5 seconds @13:00:15.46 CSV.dial CBV @13:00:15.48
CBV.dial CSV @13:00:16.27 CTV.dial :intake @13:00:16.98 Intake.select :open @13:00:17.07 CS.seek 0.000ml @13:00:40.79 Intake.select :close @13:00:40.88 CTV.dial :air @13:00:41.62 Flushing upper manifold @13:00:41.71 CSV.dial :flush @13:00:42.50 CS.seek 5.000ml @13:00:54.66 delay 5 seconds @13:00:59.67 CSV.dial :air @13:01:00.13 CS.seek 7.000ml @13:01:05.60 CSV.dial CBV @13:01:05.62
CBV.dial CSV @13:01:06.69 CTV.dial :air @13:01:06.71 CS.seek 0.000ml @13:01:23.32 delay 7 seconds @13:01:30.33 CS.seek 3.000ml @13:01:37.91 CSV.dial :waste @13:01:38.42 CS.seek 0.000ml @13:01:46.08 CSV.dial :flush @13:01:46.51 CS.seek 3.000ml @13:01:54.14 delay 5 seconds @13:01:59.14 CSV.dial :air @13:01:59.63 CS.seek 4.999ml @13:02:05.03 CSV.dial :air @13:02:05.04 CS.seek 0.000ml @13:02:17.16 delay 7 seconds @13:02:24.17 CS.seek 3.000ml @13:02:31.72 CSV.dial :waste @13:02:32.53 CS.seek 0.000ml @13:02:40.19 Garaging FlushPuck in Collection Clamp @13:02:40.25 Elbow.reconfigure TightElbow @13:02:40.28 Elbow.seek CC @13:02:40.51 Elbow.reconfigure LooseElbow @13:02:40.59 Forearm.seek CC @13:02:41.54 CC.seek sealed @13:02:42.47 Hand.grip @13:02:43.18 CC.seek open @13:02:46.47 Forearm.seek retract @13:02:47.40 Elbow.reconfigure TightElbow @13:02:47.45 Elbow.seek garage @13:02:50.24 Elbow.reconfigure LooseElbow @13:02:50.32 Forearm.reconfigure TightForearm @13:02:50.36 Forearm.seek garage @13:02:51.54 Hand.release @13:02:52.34 Forearm.reconfigure LooseForearm @13:02:52.37 Forearm.seek retract @13:02:53.50 Starting mfb @13:02:53.51 pcrBAC sampling at most 1000ml, wcr at most 1000ml @13:02:53.53 BACfans.email "Can@12:58:42, 23.6C, 46% humidity, 14.5psia, 13.644V, 0.677A, 0.677A avg, 9.24W\nCTD data unavailable\nISUS data unavailable",Subject => "pcrBAC sampling at most 1000ml, wcr at most 1000ml" @13:02:56.45
Loading sh1 puck from tube 2 into Collection Clamp @13:02:56.56 Readying the MFB @13:02:56.57 Homing MFB Syringe @13:02:56.58 AFV.select :holdingCoil @13:02:56.83
SC.reconfigure SCloose @13:02:56.89 ATV.dial :air2 @13:02:57.05
Elbow.reconfigure TightElbow @13:02:57.08 Elbow.seek 2 @13:02:57.81 AS.reconfigure ASconfig @13:02:57.84 AS.home.seek home @13:02:58.95
Forearm.reconfigure TightForearm @13:02:58.98 Forearm.seek 2 + 800 counts @13:03:00.59 SE.reconfigure SEpick @13:03:00.66 SE.seek beamBreak @13:03:01.77 SE.configure SEconfig @13:03:01.91 Elbow.seek 2 @13:03:02.08 Forearm.seek 2 + 300 counts @13:03:02.29 Forearm.reconfigure LooseForearm @13:03:02.33 Hand.grip @13:03:03.07 SE.seek down @13:03:03.21
Forearm.seek retract @13:03:04.19 Elbow.seek CC @13:03:04.23 SE.coast @13:03:05.37 AFV.select :carrierSolution @13:03:05.48 delay 2 seconds @13:03:05.65
Elbow.reconfigure LooseElbow @13:03:05.74 Forearm.reconfigure TightForearm @13:03:05.77 Forearm.seek CC @13:03:06.86 CC.seek closed @13:03:07.49 AS.seek 1.000ml @13:03:10.61
Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 2.03 Hand.release @13:03:11.34 Forearm.reconfigure LooseForearm @13:03:11.37 Forearm.seek retract @13:03:12.34 SC.coast @13:03:12.37 collectSample 1000ml w/bubble point of 55psi @13:03:12.38 Priming 7ml Sleepy.power :sampler,:ON @13:03:12.40 Intake.select :close @13:03:12.41 Exhaust.select :close @13:03:12.42 SS.hold @13:03:12.58 Calibrating pressure and temperature sensors @13:03:12.60 CTV.dial :air @13:03:12.61 SSV.dial :spit,avoiding => exhaust @13:03:12.62 SS.reconfigure SSfast @13:03:12.65 SS.seek 5.00ml @13:03:15.62 SSV.dial CBV @13:03:15.64
CBV.dial SSV @13:03:16.99 delay 3.8 seconds @13:03:20.87 SSV.dial :spit @13:03:22.21 SS.seek 0.00ml @13:03:24.64 SSV.dial CBV @13:03:25.96 CTV.dial :intake @13:03:26.72 Waiting for external sampler to finish priming... @13:03:26.73 delayUntil "13:13:12.38" @13:03:59.23 delay 1 second @13:04:00.24 AFV.select :holdingCoil @13:04:00.33 delay 1 second @13:04:01.34 ATV.dial :waste @13:04:02.24 AS.reconfigure ASnorm @13:04:02.27 AS.home.seek empty @13:04:55.43 ATV.dial :waste @13:04:55.44 ARV.dial between carrier and air1 @13:04:55.51 ASV.dial :waste1 @13:11:58.14 Session Begun @13:12:21.74 -> showlog @13:13:12.39 SSV.dial CBV @13:13:12.41
CBV.dial SSV @13:13:12.43 Allowing 55s for 7.0ml of remaining 7.0ml @13:13:12.44 Intake.select :open @13:13:14.59 Intake @37.5psia, vacuum from 20.0 to 24.0psi @13:13:14.61 SS.reconfigure forSampling @13:13:14.66 SS.seek 7.00ml @13:13:18.37 Vacuum too high -- waiting up to 40s @13:13:18.63 Intake @30.1psia, vacuum from 20.0 to 24.0psi SS.reconfigure forSampling @13:13:18.69 SS.seek 7.00ml @13:13:21.38 Stroke completed -- waiting for pressure to equalize ... @13:13:22.02 Intake.select :close @13:13:22.17 SSV.dial :exhaust @13:13:22.94 Exhaust.select :open @13:13:23.05 SS.reconfigure SSexhale @13:13:23.10 SS.seek 0.00ml @13:13:26.63 Exhaust.select :close @13:13:26.75 Sampling 1000.0ml @13:13:26.77 Intake.select :close @13:13:26.78 Exhaust.select :close @13:13:26.79 SS.reconfigure SSconfig @13:13:26.85 SS.hold @13:13:27.01 CTV.dial :intake @13:13:27.02 SSV.dial CBV @13:13:27.05
CBV.dial SSV @13:13:27.84 Allowing 185s for 25.0ml of remaining 1000.0ml @13:13:27.85 Intake.select :open @13:13:30.00 Intake @36.8psia, vacuum from 20.0 to 24.0psi @13:13:30.01 SS.reconfigure forSampling @13:13:30.07 SS.seek 25.00ml @13:13:35.92 Vacuum too high -- waiting up to 40s @13:13:36.19 Intake @30.6psia, vacuum from 20.0 to 24.0psi @13:13:36.20 SS.reconfigure forSampling @13:13:36.26 SS.seek 25.00ml @13:13:47.00 Stroke completed -- waiting for pressure to equalize ... @13:13:47.64 Intake.select :close @13:13:47.78 SSV.dial :exhaust @13:13:48.56 Exhaust.select :open @13:13:48.67 SS.reconfigure SSexhale @13:13:48.72 SS.seek 0.00ml @13:13:57.97 Exhaust.select :close @13:13:58.08 SSV.dial CBV @13:13:58.10
CBV.dial SSV @13:13:58.89 Allowing 185s for 25.0ml of remaining 975.0ml @13:13:58.90 Intake.select :open @13:14:01.06 Intake @36.7psia, vacuum from 20.0 to 24.0psi @13:14:01.07 SS.reconfigure forSampling @13:14:01.13 SS.seek 25.00ml @13:14:17.80 Stroke completed -- waiting for pressure to equalize ... @13:14:18.44 Intake.select :close @13:14:18.59 SSV.dial :exhaust @13:14:19.36 Exhaust.select :open @13:14:19.47 SS.reconfigure SSexhale @13:14:19.52 SS.seek 0.00ml @13:14:28.16 Exhaust.select :close @13:14:28.27 SSV.dial CBV @13:14:28.29
CBV.dial SSV @13:14:29.08 Allowing 185s for 25.0ml of remaining 950.0ml @13:14:29.09 Intake.select :open @13:14:31.24 Intake @36.1psia, vacuum from 20.0 to 24.0psi @13:14:31.81 SS.reconfigure forSampling @13:14:31.87 SS.seek 25.00ml @13:14:48.09 Stroke completed -- waiting for pressure to equalize ... @13:14:48.73 Intake.select :close @13:14:48.87 SSV.dial :exhaust @13:14:49.66 Exhaust.select :open @13:14:49.77 SS.reconfigure SSexhale @13:14:49.82 SS.seek 0.00ml @13:14:58.97 Exhaust.select :close @13:14:59.08 SSV.dial CBV @13:14:59.10
CBV.dial SSV @13:14:59.87 Allowing 185s for 25.0ml of remaining 925.0ml @13:14:59.89 Intake.select :open @13:15:02.05 Intake @35.9psia, vacuum from 20.0 to 24.0psi @13:15:02.06 SS.reconfigure forSampling @13:15:02.12 SS.seek 25.00ml @13:15:14.10 Vacuum too high -- waiting up to 40s @13:15:14.37 Intake @30.3psia, vacuum from 20.0 to 24.0psi @13:15:14.38 SS.reconfigure forSampling @13:15:14.44 SS.seek 25.00ml @13:15:19.95 Stroke completed -- waiting for pressure to equalize ... @13:15:20.59 Intake.select :close @13:15:20.73 SSV.dial :exhaust @13:15:21.51 Exhaust.select :open @13:15:21.62 SS.reconfigure SSexhale @13:15:21.67 SS.seek 0.00ml @13:15:30.89 Exhaust.select :close @13:15:31.00 SSV.dial CBV @13:15:31.02
CBV.dial SSV @13:15:31.81 Allowing 185s for 25.0ml of remaining 900.0ml @13:15:31.82 Intake.select :open @13:15:33.97 Intake @35.8psia, vacuum from 20.0 to 24.0psi @13:15:33.98 SS.reconfigure forSampling @13:15:34.04 SS.seek 25.00ml @13:15:52.62 Stroke completed -- waiting for pressure to equalize ... @13:15:53.26 Intake.select :close @13:15:53.40 SSV.dial :exhaust @13:15:54.18 Exhaust.select :open @13:15:54.29 SS.reconfigure SSexhale @13:15:54.34 SS.seek 0.00ml @13:16:03.34 Exhaust.select :close @13:16:03.45 SSV.dial CBV @13:16:03.47
CBV.dial SSV @13:16:04.26 Allowing 185s for 25.0ml of remaining 875.0ml @13:16:04.27 Intake.select :open @13:16:06.45 Intake @35.9psia, vacuum from 20.0 to 24.0psi @13:16:06.46 SS.reconfigure forSampling @13:16:06.52 SS.seek 25.00ml @13:16:25.01 Stroke completed -- waiting for pressure to equalize ... @13:16:25.65 Intake.select :close @13:16:25.80 SSV.dial :exhaust @13:16:26.57 Exhaust.select :open @13:16:26.68 SS.reconfigure SSexhale @13:16:26.73 SS.seek 0.00ml @13:16:35.52 Exhaust.select :close @13:16:35.63 SSV.dial CBV @13:16:35.65
CBV.dial SSV @13:16:36.45 Allowing 185s for 25.0ml of remaining 850.0ml @13:16:36.46 Intake.select :open @13:16:38.61 Intake @36.2psia, vacuum from 20.0 to 24.0psi @13:16:38.62 SS.reconfigure forSampling @13:16:38.68 SS.seek 25.00ml @13:16:47.16 Vacuum too high -- waiting up to 40s @13:16:47.43 Intake @31.0psia, vacuum from 20.0 to 24.0psi @13:16:47.44 SS.reconfigure forSampling @13:16:47.50 SS.seek 25.00ml @13:16:59.59 Stroke completed -- waiting for pressure to equalize ... @13:17:00.23 Intake.select :close @13:17:00.38 SSV.dial :exhaust @13:17:01.16 Exhaust.select :open @13:17:01.27 SS.reconfigure SSexhale @13:17:01.32 SS.seek 0.00ml @13:17:10.59 Exhaust.select :close @13:17:10.70 SSV.dial CBV @13:17:10.72
CBV.dial SSV @13:17:11.52 Allowing 185s for 25.0ml of remaining 825.0ml @13:17:11.53 Intake.select :open @13:17:13.68 Intake @36.5psia, vacuum from 20.0 to 24.0psi @13:17:13.69 SS.reconfigure forSampling @13:17:13.75 SS.seek 25.00ml @13:17:32.10 Vacuum too high -- waiting up to 40s @13:17:32.37 Intake @32.2psia, vacuum from 20.0 to 24.0psi @13:17:32.38 SS.reconfigure forSampling @13:17:32.44 SS.seek 25.00ml @13:17:36.35 Stroke completed -- waiting for pressure to equalize ... @13:17:36.98 Intake.select :close @13:17:37.13 SSV.dial :exhaust @13:17:37.91 Exhaust.select :open @13:17:38.02 SS.reconfigure SSexhale @13:17:38.07 SS.seek 0.00ml @13:17:46.71 Exhaust.select :close @13:17:46.82 SSV.dial CBV @13:17:46.84
CBV.dial SSV @13:17:47.61 Allowing 185s for 25.0ml of remaining 800.0ml @13:17:47.62 Intake.select :open @13:17:49.77 Intake @36.0psia, vacuum from 20.0 to 24.0psi @13:17:49.78 SS.reconfigure forSampling @13:17:49.84 SS.seek 25.00ml @13:18:07.81 Session Ended @13:18:14.80
Stroke completed -- waiting for pressure to equalize ... @13:18:15.44 Intake.select :close @13:18:15.57 SSV.dial :exhaust @13:18:16.33 Exhaust.select :open @13:18:16.42 SS.reconfigure SSexhale @13:18:16.46 SS.seek 0.00ml @13:18:25.36 Exhaust.select :close @13:18:25.45 SSV.dial CBV @13:18:25.47
CBV.dial SSV @13:18:26.25 Allowing 185s for 25.0ml of remaining 775.0ml @13:18:26.26 Intake.select :open @13:18:28.39 Intake @36.1psia, vacuum from 20.0 to 24.0psi @13:18:28.40 SS.reconfigure forSampling @13:18:28.44 SS.seek 25.00ml @13:18:52.40 Vacuum too high -- waiting up to 40s @13:18:52.67 Intake @33.0psia, vacuum from 20.0 to 24.0psi @13:18:52.68 SS.reconfigure forSampling @13:18:52.71 SS.seek 25.00ml @13:18:53.44 Stroke completed -- waiting for pressure to equalize ... @13:18:54.08 Intake.select :close @13:18:54.21 SSV.dial :exhaust @13:18:54.99 Exhaust.select :open @13:18:55.10 SS.reconfigure SSexhale @13:18:55.14 SS.seek 0.00ml @13:19:04.02 Exhaust.select :close @13:19:04.11 SSV.dial CBV @13:19:04.13
CBV.dial SSV @13:19:04.92 Allowing 185s for 25.0ml of remaining 750.0ml @13:19:04.93 Intake.select :open @13:19:07.06 Intake @36.2psia, vacuum from 20.0 to 24.0psi @13:19:07.07 SS.reconfigure forSampling @13:19:07.11 SS.seek 25.00ml @13:19:37.30 Stroke completed -- waiting for pressure to equalize ... @13:19:37.94 Intake.select :close @13:19:38.07 SSV.dial :exhaust @13:19:38.84 Exhaust.select :open @13:19:38.93 SS.reconfigure SSexhale @13:19:38.97 SS.seek 0.00ml @13:19:48.11 Exhaust.select :close @13:19:48.20 SSV.dial CBV @13:19:48.22
CBV.dial SSV @13:19:49.00 Allowing 185s for 25.0ml of remaining 725.0ml @13:19:49.01 Intake.select :open @13:19:51.14 Intake @35.6psia, vacuum from 20.0 to 24.0psi @13:19:51.15 SS.reconfigure forSampling @13:19:51.19 SS.seek 25.00ml @13:20:21.29 Stroke completed -- waiting for pressure to equalize ... @13:20:21.92 Intake.select :close @13:20:22.05 SSV.dial :exhaust @13:20:22.82 Exhaust.select :open @13:20:22.91 SS.reconfigure SSexhale @13:20:22.95 SS.seek 0.00ml @13:20:31.75 Exhaust.select :close @13:20:31.84 SSV.dial CBV @13:20:31.86
CBV.dial SSV @13:20:32.64 Allowing 185s for 25.0ml of remaining 700.0ml @13:20:32.65 Intake.select :open @13:20:34.78 Intake @35.5psia, vacuum from 20.0 to 24.0psi @13:20:34.79 SS.reconfigure forSampling @13:20:34.83 SS.seek 25.00ml @13:20:49.02 Vacuum too high -- waiting up to 40s @13:20:49.94 Intake @36.4psia, vacuum from 20.0 to 24.0psi @13:20:49.95 SS.reconfigure forSampling @13:20:49.99 SS.seek 25.00ml @13:21:11.85 Stroke completed -- waiting for pressure to equalize ... @13:21:12.49 Intake.select :close @13:21:12.62 SSV.dial :exhaust @13:21:13.38 Exhaust.select :open @13:21:13.47 SS.reconfigure SSexhale @13:21:13.51 SS.seek 0.00ml @13:21:22.32 Exhaust.select :close @13:21:22.41 SSV.dial CBV @13:21:22.43
CBV.dial SSV @13:21:23.21 Allowing 185s for 25.0ml of remaining 675.0ml @13:21:23.22 Intake.select :open @13:21:25.35 Intake @36.1psia, vacuum from 20.0 to 24.0psi @13:21:25.36 SS.reconfigure forSampling @13:21:25.40 SS.seek 25.00ml @13:22:05.96 Stroke completed -- waiting for pressure to equalize ... @13:22:06.60 Intake.select :close @13:22:06.73 SSV.dial :exhaust @13:22:07.51 Exhaust.select :open @13:22:07.60 SS.reconfigure SSexhale @13:22:07.64 SS.seek 0.00ml @13:22:16.47 Exhaust.select :close @13:22:16.56 SSV.dial CBV @13:22:16.58
CBV.dial SSV @13:22:17.35 Allowing 185s for 25.0ml of remaining 650.0ml @13:22:17.36 Intake.select :open @13:22:19.49 Intake @35.9psia, vacuum from 20.0 to 24.0psi @13:22:19.50 SS.reconfigure forSampling @13:22:19.54 SS.seek 25.00ml @13:22:43.25 Vacuum too high -- waiting up to 40s @13:22:43.52 Intake @34.7psia, vacuum from 20.0 to 24.0psi @13:22:43.53 SS.reconfigure forSampling @13:22:43.56 SS.seek 25.00ml @13:23:07.68 Stroke completed -- waiting for pressure to equalize ... @13:23:08.32 Intake.select :close @13:23:08.45 SSV.dial :exhaust @13:23:09.23 Exhaust.select :open @13:23:09.33 SS.reconfigure SSexhale @13:23:09.37 SS.seek 0.00ml @13:23:18.15 Exhaust.select :close @13:23:18.24 SSV.dial CBV @13:23:18.26
CBV.dial SSV @13:23:19.04 Allowing 185s for 25.0ml of remaining 625.0ml @13:23:19.05 Intake.select :open @13:23:21.18 Intake @36.4psia, vacuum from 20.0 to 24.0psi @13:23:21.19 SS.reconfigure forSampling @13:23:21.23 SS.seek 25.00ml @13:24:20.46 Stroke completed -- waiting for pressure to equalize ... @13:24:21.10 Intake.select :close @13:24:21.23 SSV.dial :exhaust @13:24:21.99 Exhaust.select :open @13:24:22.08 SS.reconfigure SSexhale @13:24:22.12 SS.seek 0.00ml @13:24:31.01 Exhaust.select :close @13:24:31.10 SSV.dial CBV @13:24:31.12
CBV.dial SSV @13:24:31.90 Allowing 185s for 25.0ml of remaining 600.0ml @13:24:31.91 Intake.select :open @13:24:34.04 Intake @36.8psia, vacuum from 20.0 to 24.0psi @13:24:34.05 SS.reconfigure forSampling @13:24:34.09 SS.seek 25.00ml @13:25:34.24 Stroke completed -- waiting for pressure to equalize ... @13:25:34.87 Intake.select :close @13:25:35.01 SSV.dial :exhaust @13:25:35.77 Exhaust.select :open @13:25:35.87 SS.reconfigure SSexhale @13:25:35.91 SS.seek 0.00ml @13:25:44.71 Exhaust.select :close @13:25:44.80 SSV.dial CBV @13:25:44.82
CBV.dial SSV @13:25:45.60 Allowing 185s for 25.0ml of remaining 575.0ml @13:25:45.61 Intake.select :open @13:25:47.76 Intake @36.9psia, vacuum from 20.0 to 24.0psi @13:25:47.77 SS.reconfigure forSampling @13:25:47.81 SS.seek 25.00ml @13:26:28.64 Vacuum too high -- waiting up to 40s @13:26:28.91 Intake @35.7psia, vacuum from 20.0 to 24.0psi @13:26:28.92 SS.reconfigure forSampling @13:26:28.95 SS.seek 25.00ml @13:27:03.42 Stroke completed -- waiting for pressure to equalize ... @13:27:04.65 Intake.select :close @13:27:04.78 SSV.dial :exhaust @13:27:05.56 Exhaust.select :open @13:27:05.66 SS.reconfigure SSexhale @13:27:05.70 SS.seek 0.00ml @13:27:14.27 Exhaust.select :close @13:27:14.36 SSV.dial CBV @13:27:14.38
CBV.dial SSV @13:27:15.17 Allowing 185s for 25.0ml of remaining 550.0ml @13:27:15.18 Intake.select :open @13:27:17.31 Intake @37.5psia, vacuum from 20.0 to 24.0psi @13:27:17.32 SS.reconfigure forSampling @13:27:17.36 SS.seek 25.00ml @13:29:07.65 Stroke completed -- waiting for pressure to equalize ... @13:29:08.86 Intake.select :close @13:29:09.00 SSV.dial :exhaust @13:29:09.77 Exhaust.select :open @13:29:09.87 SS.reconfigure SSexhale @13:29:09.91 SS.seek 0.00ml @13:29:18.73 Exhaust.select :close @13:29:18.82 SSV.dial CBV @13:29:18.84
CBV.dial SSV @13:29:19.62 Allowing 185s for 25.0ml of remaining 525.0ml @13:29:19.63 Intake.select :open @13:29:21.76 Intake @37.8psia, vacuum from 20.0 to 24.0psi @13:29:21.77 SS.reconfigure forSampling @13:29:21.81 SS.seek 25.00ml @13:31:12.10 Stroke completed -- waiting for pressure to equalize ... @13:31:13.31 Intake.select :close @13:31:13.45 SSV.dial :exhaust @13:31:14.22 Exhaust.select :open @13:31:14.32 SS.reconfigure SSexhale @13:31:14.36 SS.seek 0.00ml @13:31:23.11 Exhaust.select :close @13:31:23.20 SSV.dial CBV @13:31:23.22
CBV.dial SSV @13:31:24.00 Allowing 185s for 25.0ml of remaining 500.0ml @13:31:24.01 Intake.select :open @13:31:26.61 Intake @37.6psia, vacuum from 20.0 to 24.0psi @13:31:26.62 SS.reconfigure forSampling @13:31:26.66 SS.seek 25.00ml @13:33:17.03 Stroke completed -- waiting for pressure to equalize ... @13:33:18.26 Intake.select :close @13:33:18.40 SSV.dial :exhaust @13:33:19.17 Exhaust.select :open @13:33:19.28 SS.reconfigure SSexhale @13:33:19.32 SS.seek 0.00ml @13:33:28.20 Exhaust.select :close @13:33:28.29 SSV.dial CBV @13:33:28.31
CBV.dial SSV @13:33:29.09 Allowing 185s for 25.0ml of remaining 475.0ml @13:33:29.10 Intake.select :open @13:33:31.23 Intake @37.9psia, vacuum from 20.0 to 24.0psi @13:33:31.24 SS.reconfigure forSampling @13:33:31.28 SS.seek 25.00ml @13:35:22.41 Stroke completed -- waiting for pressure to equalize ... @13:35:23.05 Intake.select :close @13:35:23.18 SSV.dial :exhaust @13:35:23.94 Exhaust.select :open @13:35:24.03 SS.reconfigure SSexhale @13:35:24.07 SS.seek 0.00ml @13:35:32.69 Exhaust.select :close @13:35:32.78 SSV.dial CBV @13:35:32.80
CBV.dial SSV @13:35:33.58 Allowing 185s for 25.0ml of remaining 450.0ml @13:35:33.59 Intake.select :open @13:35:35.72 Intake @37.0psia, vacuum from 20.0 to 24.0psi @13:35:35.73 SS.reconfigure forSampling @13:35:35.77 SS.seek 25.00ml @13:37:26.17 Stroke completed -- waiting for pressure to equalize ... @13:37:27.99 Intake.select :close @13:37:28.12 SSV.dial :exhaust @13:37:28.89 Exhaust.select :open @13:37:28.98 SS.reconfigure SSexhale @13:37:29.02 SS.seek 0.00ml @13:37:37.81 Exhaust.select :close @13:37:37.90 SSV.dial CBV @13:37:37.92
CBV.dial SSV @13:37:38.70 Allowing 185s for 25.0ml of remaining 425.0ml @13:37:38.71 Intake.select :open @13:37:40.84 Intake @37.4psia, vacuum from 20.0 to 24.0psi @13:37:40.85 SS.reconfigure forSampling @13:37:40.89 SS.seek 25.00ml @13:38:57.69 Vacuum too high -- waiting up to 40s @13:38:57.96 Intake @36.6psia, vacuum from 20.0 to 24.0psi @13:38:57.97 SS.reconfigure forSampling @13:38:58.00 SS.seek 25.00ml @13:39:30.17 Vacuum too high -- waiting up to 40s @13:39:30.43 Intake @36.7psia, vacuum from 20.0 to 24.0psi @13:39:30.44 SS.reconfigure forSampling @13:39:30.48 SS.seek 25.00ml @13:39:32.25 Stroke completed -- waiting for pressure to equalize ... @13:39:34.70 Intake.select :close @13:39:34.83 SSV.dial :exhaust @13:39:35.61 Exhaust.select :open @13:39:35.70 SS.reconfigure SSexhale @13:39:35.74 SS.seek 0.00ml @13:39:44.42 Exhaust.select :close @13:39:44.51 SSV.dial CBV @13:39:44.53
CBV.dial SSV @13:39:45.31 Allowing 185s for 25.0ml of remaining 400.0ml @13:39:45.32 Intake.select :open @13:39:47.45 Intake @36.7psia, vacuum from 20.0 to 24.0psi @13:39:47.46 SS.reconfigure forSampling @13:39:47.50 SS.seek 25.00ml @13:40:30.39 Vacuum too high -- waiting up to 40s @13:40:30.66 Intake @36.9psia, vacuum from 20.0 to 24.0psi @13:40:30.67 SS.reconfigure forSampling @13:40:30.70 SS.seek 25.00ml @13:40:36.85 Vacuum too high -- waiting up to 40s @13:40:37.13 SS.seek 25.00ml @13:40:42.73 Vacuum too high -- waiting up to 40s @13:40:42.99 Intake @36.8psia, vacuum from 20.0 to 24.0psi @13:40:43.00 SS.reconfigure forSampling @13:40:43.04 SS.seek 25.00ml @13:40:46.07 Vacuum too high -- waiting up to 40s @13:40:46.34 Intake @36.8psia, vacuum from 20.0 to 24.0psi @13:40:46.35 SS.reconfigure forSampling @13:40:46.38 SS.seek 25.00ml @13:40:52.01 Vacuum too high -- waiting up to 40s @13:40:52.28 Intake @36.8psia, vacuum from 20.0 to 24.0psi @13:40:52.29 SS.reconfigure forSampling @13:40:52.32 SS.seek 25.00ml @13:40:54.55 Vacuum too high -- waiting up to 40s @13:40:54.81 Intake @36.8psia, vacuum from 20.0 to 24.0psi @13:40:54.82 SS.reconfigure forSampling @13:40:54.85 SS.seek 25.00ml @13:40:57.95 Vacuum too high -- waiting up to 40s @13:40:58.21 Intake @37.0psia, vacuum from 20.0 to 24.0psi @13:40:58.22 SS.reconfigure forSampling @13:40:58.26 SS.seek 25.00ml @13:41:02.79 Vacuum too high -- waiting up to 40s @13:41:03.05 Intake @36.9psia, vacuum from 20.0 to 24.0psi @13:41:03.06 SS.reconfigure forSampling @13:41:03.10 SS.seek 25.00ml @13:41:05.30 Vacuum too high -- waiting up to 40s @13:41:05.56 Intake @37.0psia, vacuum from 20.0 to 24.0psi @13:41:05.57 SS.reconfigure forSampling @13:41:05.60 SS.seek 25.00ml @13:41:08.63 Vacuum too high -- waiting up to 40s @13:41:08.90 Intake @37.0psia, vacuum from 20.0 to 24.0psi @13:41:08.91 SS.reconfigure forSampling @13:41:08.95 SS.seek 25.00ml @13:41:11.08 Vacuum too high -- waiting up to 40s @13:41:11.34 Intake @37.1psia, vacuum from 20.0 to 24.0psi @13:41:11.35 SS.reconfigure forSampling @13:41:11.39 SS.seek 25.00ml @13:41:13.45 Vacuum too high -- waiting up to 40s @13:41:13.72 SS.seek 25.00ml @13:41:15.74 Vacuum too high -- waiting up to 40s @13:41:16.00 Intake @37.2psia, vacuum from 20.0 to 24.0psi @13:41:16.01 SS.reconfigure forSampling @13:41:16.04 SS.seek 25.00ml @13:41:17.77 Vacuum too high -- waiting up to 40s @13:41:18.03 Intake @37.0psia, vacuum from 20.0 to 24.0psi @13:41:18.04 SS.reconfigure forSampling @13:41:18.07 SS.seek 25.00ml @13:41:20.17 Vacuum too high -- waiting up to 40s @13:41:20.43 Intake @36.9psia, vacuum from 20.0 to 24.0psi @13:41:20.44 SS.reconfigure forSampling @13:41:20.48 SS.seek 25.00ml @13:41:22.24 Vacuum too high -- waiting up to 40s @13:41:22.50 Intake @37.0psia, vacuum from 20.0 to 24.0psi @13:41:22.51 SS.reconfigure forSampling @13:41:22.54 SS.seek 25.00ml @13:41:24.39 Vacuum too high -- waiting up to 40s @13:41:24.65 Intake @36.9psia, vacuum from 20.0 to 24.0psi @13:41:24.66 SS.reconfigure forSampling @13:41:24.70 SS.seek 25.00ml @13:41:26.61 Vacuum too high -- waiting up to 40s @13:41:26.87 Intake @37.4psia, vacuum from 20.0 to 24.0psi @13:41:26.88 SS.reconfigure forSampling @13:41:26.91 SS.seek 25.00ml @13:41:28.86 Vacuum too high -- waiting up to 40s @13:41:29.12 Intake @37.0psia, vacuum from 20.0 to 24.0psi @13:41:29.13 SS.reconfigure forSampling @13:41:29.16 SS.seek 25.00ml @13:41:31.30 Vacuum too high -- waiting up to 40s @13:41:31.56 Intake @37.1psia, vacuum from 20.0 to 24.0psi @13:41:31.57 SS.reconfigure forSampling @13:41:31.60 SS.seek 25.00ml @13:41:33.36 Vacuum too high -- waiting up to 40s @13:41:33.62 Intake @36.7psia, vacuum from 20.0 to 24.0psi @13:41:33.63 SS.reconfigure forSampling @13:41:33.66 SS.seek 25.00ml @13:41:35.64 Vacuum too high -- waiting up to 40s @13:41:35.90 Intake @36.8psia, vacuum from 20.0 to 24.0psi @13:41:35.91 SS.reconfigure forSampling @13:41:35.94 SS.seek 25.00ml @13:41:37.80 Vacuum too high -- waiting up to 40s @13:41:38.06 Intake @37.2psia, vacuum from 20.0 to 24.0psi @13:41:38.07 SS.reconfigure forSampling @13:41:38.10 SS.seek 25.00ml @13:41:39.89 Vacuum too high -- waiting up to 40s @13:41:40.15 Intake @37.1psia, vacuum from 20.0 to 24.0psi @13:41:40.16 SS.reconfigure forSampling @13:41:40.19 SS.seek 25.00ml @13:41:41.89 Vacuum too high -- waiting up to 40s @13:41:42.15 Intake @36.8psia, vacuum from 20.0 to 24.0psi @13:41:42.16 SS.reconfigure forSampling @13:41:42.19 SS.seek 25.00ml @13:41:44.08 Vacuum too high -- waiting up to 40s @13:41:44.77 Intake @37.1psia, vacuum from 20.0 to 24.0psi @13:41:44.78 SS.reconfigure forSampling @13:41:44.82 SS.seek 25.00ml @13:41:47.55 Vacuum too high -- waiting up to 40s @13:41:47.81 Intake @37.3psia, vacuum from 20.0 to 24.0psi @13:41:47.82 SS.reconfigure forSampling @13:41:47.86 SS.seek 25.00ml @13:41:49.64 Vacuum too high -- waiting up to 40s @13:41:50.00 Intake @36.9psia, vacuum from 20.0 to 24.0psi @13:41:50.01 SS.reconfigure forSampling @13:41:50.04 SS.seek 25.00ml @13:41:51.98 Vacuum too high -- waiting up to 40s @13:41:52.24 Intake @36.7psia, vacuum from 20.0 to 24.0psi @13:41:52.25 SS.reconfigure forSampling @13:41:52.28 SS.seek 25.00ml @13:41:53.76 Stroke completed -- waiting for pressure to equalize ... @13:41:55.57 Intake.select :close @13:41:55.70 SSV.dial :exhaust @13:41:56.46 Exhaust.select :open @13:41:56.55 SS.reconfigure SSexhale @13:41:56.59 SS.seek 0.00ml @13:42:04.99 Exhaust.select :close @13:42:05.08 SSV.dial CBV @13:42:05.10
CBV.dial SSV @13:42:05.88 Allowing 185s for 25.0ml of remaining 375.0ml @13:42:05.89 Intake.select :open @13:42:08.02 Intake @36.8psia, vacuum from 20.0 to 24.0psi @13:42:08.03 SS.reconfigure forSampling @13:42:08.07 SS.seek 25.00ml @13:42:11.20 Vacuum too high -- waiting up to 40s @13:42:11.46 Intake @36.5psia, vacuum from 20.0 to 24.0psi @13:42:11.47 SS.reconfigure forSampling @13:42:11.50 SS.seek 25.00ml @13:42:15.82 Vacuum too high -- waiting up to 40s @13:42:16.60 Intake @37.2psia, vacuum from 20.0 to 24.0psi @13:42:16.61 SS.reconfigure forSampling @13:42:16.64 SS.seek 25.00ml @13:42:19.20 Vacuum too high -- waiting up to 40s @13:42:19.47 Intake @36.7psia, vacuum from 20.0 to 24.0psi @13:42:19.48 SS.reconfigure forSampling @13:42:19.52 SS.seek 25.00ml @13:42:22.11 Vacuum too high -- waiting up to 40s @13:42:22.37 Intake @37.2psia, vacuum from 20.0 to 24.0psi @13:42:22.38 SS.reconfigure forSampling @13:42:22.42 SS.seek 25.00ml @13:42:24.54 Vacuum too high -- waiting up to 40s @13:42:24.81 Intake @36.8psia, vacuum from 20.0 to 24.0psi @13:42:24.82 SS.reconfigure forSampling @13:42:24.86 SS.seek 25.00ml @13:42:28.97 Vacuum too high -- waiting up to 40s @13:42:29.25 Intake @37.2psia, vacuum from 20.0 to 24.0psi @13:42:29.26 SS.reconfigure forSampling @13:42:29.29 SS.seek 25.00ml @13:42:31.61 Vacuum too high -- waiting up to 40s @13:42:31.87 Intake @37.4psia, vacuum from 20.0 to 24.0psi @13:42:31.88 SS.reconfigure forSampling @13:42:31.92 SS.seek 25.00ml @13:42:34.08 Vacuum too high -- waiting up to 40s @13:42:34.34 Intake @36.7psia, vacuum from 20.0 to 24.0psi @13:42:34.35 SS.reconfigure forSampling @13:42:34.39 SS.seek 25.00ml @13:42:36.64 Vacuum too high -- waiting up to 40s @13:42:36.91 SS.seek 25.00ml @13:42:39.01 Vacuum too high -- waiting up to 40s @13:42:39.27 Intake @36.6psia, vacuum from 20.0 to 24.0psi @13:42:39.28 SS.reconfigure forSampling @13:42:39.32 SS.seek 25.00ml @13:42:42.17 Vacuum too high -- waiting up to 40s @13:42:42.43 Intake @37.5psia, vacuum from 20.0 to 24.0psi @13:42:42.44 SS.reconfigure forSampling @13:42:42.48 SS.seek 25.00ml @13:42:44.23 Vacuum too high -- waiting up to 40s @13:42:44.49 Intake @36.5psia, vacuum from 20.0 to 24.0psi @13:42:44.50 SS.reconfigure forSampling @13:42:44.53 SS.seek 25.00ml @13:42:46.82 Vacuum too high -- waiting up to 40s @13:42:47.08 Intake @37.2psia, vacuum from 20.0 to 24.0psi @13:42:47.09 SS.reconfigure forSampling @13:42:47.13 SS.seek 25.00ml @13:42:48.95 Vacuum too high -- waiting up to 40s @13:42:49.22 Intake @37.0psia, vacuum from 20.0 to 24.0psi @13:42:49.23 SS.reconfigure forSampling @13:42:49.27 SS.seek 25.00ml @13:42:51.32 Vacuum too high -- waiting up to 40s @13:42:51.58 Intake @37.2psia, vacuum from 20.0 to 24.0psi @13:42:51.59 SS.reconfigure forSampling @13:42:51.62 SS.seek 25.00ml @13:42:53.48 Vacuum too high -- waiting up to 40s @13:42:53.74 Intake @37.1psia, vacuum from 20.0 to 24.0psi @13:42:53.75 SS.reconfigure forSampling @13:42:53.78 SS.seek 25.00ml @13:42:55.57 Vacuum too high -- waiting up to 40s @13:42:55.83 Intake @36.8psia, vacuum from 20.0 to 24.0psi @13:42:55.84 SS.reconfigure forSampling @13:42:55.88 SS.seek 25.00ml @13:42:57.82 Vacuum too high -- waiting up to 40s @13:42:58.17 Intake @36.8psia, vacuum from 20.0 to 24.0psi @13:42:58.18 SS.reconfigure forSampling @13:42:58.22 SS.seek 25.00ml @13:43:00.70 Vacuum too high -- waiting up to 40s @13:43:00.96 Intake @37.5psia, vacuum from 20.0 to 24.0psi @13:43:00.97 SS.reconfigure forSampling @13:43:01.00 SS.seek 25.00ml @13:43:02.35 Vacuum too high -- waiting up to 40s @13:43:02.62 Intake @36.8psia, vacuum from 20.0 to 24.0psi @13:43:02.63 SS.reconfigure forSampling @13:43:02.66 SS.seek 25.00ml @13:43:04.35 Vacuum too high -- waiting up to 40s @13:43:04.61 Intake @37.0psia, vacuum from 20.0 to 24.0psi @13:43:04.62 SS.reconfigure forSampling @13:43:04.65 SS.seek 25.00ml @13:43:06.07 Vacuum too high -- waiting up to 40s @13:43:06.33 Intake @36.7psia, vacuum from 20.0 to 24.0psi @13:43:06.34 SS.reconfigure forSampling @13:43:06.37 SS.seek 25.00ml @13:43:07.92 Vacuum too high -- waiting up to 40s @13:43:08.18 Intake @37.0psia, vacuum from 20.0 to 24.0psi @13:43:08.19 SS.reconfigure forSampling @13:43:08.22 SS.seek 25.00ml @13:43:09.76 Vacuum too high -- waiting up to 40s @13:43:10.02 Intake @37.0psia, vacuum from 20.0 to 24.0psi @13:43:10.03 SS.reconfigure forSampling @13:43:10.06 SS.seek 25.00ml @13:43:11.79 Vacuum too high -- waiting up to 40s @13:43:12.05 Intake @37.2psia, vacuum from 20.0 to 24.0psi @13:43:12.06 SS.reconfigure forSampling @13:43:12.09 SS.seek 25.00ml @13:43:13.51 Vacuum too high -- waiting up to 40s @13:43:13.77 Intake @36.5psia, vacuum from 20.0 to 24.0psi @13:43:13.78 SS.reconfigure forSampling @13:43:13.81 SS.seek 25.00ml @13:43:15.39 Vacuum too high -- waiting up to 40s @13:43:16.16 Intake @37.0psia, vacuum from 20.0 to 24.0psi @13:43:16.17 SS.reconfigure forSampling @13:43:16.20 SS.seek 25.00ml @13:43:18.54 Vacuum too high -- waiting up to 40s @13:43:18.80 Intake @37.1psia, vacuum from 20.0 to 24.0psi @13:43:18.81 SS.reconfigure forSampling @13:43:18.85 SS.seek 25.00ml @13:43:20.35 Vacuum too high -- waiting up to 40s @13:43:20.61 Intake @36.8psia, vacuum from 20.0 to 24.0psi @13:43:20.62 SS.reconfigure forSampling @13:43:20.66 SS.seek 25.00ml @13:43:22.13 Vacuum too high -- waiting up to 40s @13:43:22.39 Intake @36.7psia, vacuum from 20.0 to 24.0psi @13:43:22.40 SS.reconfigure forSampling @13:43:22.43 SS.seek 25.00ml @13:43:23.95 Vacuum too high -- waiting up to 40s @13:43:24.21 Intake @37.2psia, vacuum from 20.0 to 24.0psi @13:43:24.22 SS.reconfigure forSampling @13:43:24.26 SS.seek 25.00ml @13:43:25.79 Vacuum too high -- waiting up to 40s @13:43:26.05 Intake @37.1psia, vacuum from 20.0 to 24.0psi @13:43:26.06 SS.reconfigure forSampling @13:43:26.10 SS.seek 25.00ml @13:43:27.38 Vacuum too high -- waiting up to 40s @13:43:27.64 Intake @36.9psia, vacuum from 20.0 to 24.0psi @13:43:27.65 SS.reconfigure forSampling @13:43:27.68 SS.seek 25.00ml @13:43:29.20 Vacuum too high -- waiting up to 40s @13:43:29.47 Intake @37.1psia, vacuum from 20.0 to 24.0psi @13:43:29.48 SS.reconfigure forSampling @13:43:29.51 SS.seek 25.00ml @13:43:31.04 Vacuum too high -- waiting up to 40s @13:43:31.30 Intake @37.3psia, vacuum from 20.0 to 24.0psi @13:43:31.31 SS.reconfigure forSampling @13:43:31.34 SS.seek 25.00ml @13:43:32.73 Vacuum too high -- waiting up to 40s @13:43:32.99 Intake @36.9psia, vacuum from 20.0 to 24.0psi @13:43:33.00 SS.reconfigure forSampling @13:43:33.03 SS.seek 25.00ml @13:43:34.54 Vacuum too high -- waiting up to 40s @13:43:34.80 Intake @36.7psia, vacuum from 20.0 to 24.0psi @13:43:34.81 SS.reconfigure forSampling @13:43:34.84 SS.seek 25.00ml @13:43:36.26 Vacuum too high -- waiting up to 40s @13:43:36.52 Intake @36.8psia, vacuum from 20.0 to 24.0psi @13:43:36.53 SS.reconfigure forSampling @13:43:36.56 SS.seek 25.00ml @13:43:37.98 Vacuum too high -- waiting up to 40s @13:43:38.24 Intake @36.9psia, vacuum from 20.0 to 24.0psi @13:43:38.25 SS.reconfigure forSampling @13:43:38.28 SS.seek 25.00ml @13:43:39.76 Vacuum too high -- waiting up to 40s @13:43:40.02 Intake @37.0psia, vacuum from 20.0 to 24.0psi @13:43:40.03 SS.reconfigure forSampling @13:43:40.06 SS.seek 25.00ml @13:43:41.31 Vacuum too high -- waiting up to 40s @13:43:41.58 Intake @37.2psia, vacuum from 20.0 to 24.0psi @13:43:41.59 SS.reconfigure forSampling @13:43:41.62 SS.seek 25.00ml @13:43:42.92 Vacuum too high -- waiting up to 40s @13:43:43.19 Intake @37.3psia, vacuum from 20.0 to 24.0psi @13:43:43.20 SS.reconfigure forSampling @13:43:43.23 SS.seek 25.00ml @13:43:44.54 Vacuum too high -- waiting up to 40s @13:43:44.80 Intake @37.1psia, vacuum from 20.0 to 24.0psi @13:43:44.81 SS.reconfigure forSampling @13:43:44.85 SS.seek 25.00ml @13:43:46.16 Vacuum too high -- waiting up to 40s @13:43:46.42 Intake @36.8psia, vacuum from 20.0 to 24.0psi @13:43:46.43 SS.reconfigure forSampling @13:43:46.46 SS.seek 25.00ml @13:43:47.76 Vacuum too high -- waiting up to 40s @13:43:48.02 Intake @36.9psia, vacuum from 20.0 to 24.0psi @13:43:48.03 SS.reconfigure forSampling @13:43:48.06 SS.seek 25.00ml @13:43:49.41 Vacuum too high -- waiting up to 40s @13:43:49.67 Intake @36.7psia, vacuum from 20.0 to 24.0psi @13:43:49.68 SS.reconfigure forSampling @13:43:49.71 SS.seek 25.00ml @13:43:50.95 Vacuum too high -- waiting up to 40s @13:43:51.21 Intake @36.7psia, vacuum from 20.0 to 24.0psi @13:43:51.22 SS.reconfigure forSampling @13:43:51.25 SS.seek 25.00ml @13:43:52.48 Vacuum too high -- waiting up to 40s @13:43:52.74 Intake @36.9psia, vacuum from 20.0 to 24.0psi @13:43:52.75 SS.reconfigure forSampling @13:43:52.78 SS.seek 25.00ml @13:43:54.32 Vacuum too high -- waiting up to 40s @13:43:54.58 Intake @37.7psia, vacuum from 20.0 to 24.0psi @13:43:54.59 SS.reconfigure forSampling @13:43:54.62 SS.seek 25.00ml @13:43:55.88 Vacuum too high -- waiting up to 40s @13:43:56.14 Intake @37.2psia, vacuum from 20.0 to 24.0psi @13:43:56.15 SS.reconfigure forSampling @13:43:56.18 SS.seek 25.00ml @13:43:57.41 Vacuum too high -- waiting up to 40s @13:43:57.67 Intake @36.9psia, vacuum from 20.0 to 24.0psi @13:43:57.68 SS.reconfigure forSampling @13:43:57.71 SS.seek 25.00ml @13:43:59.52 Vacuum too high -- waiting up to 40s @13:43:59.79 Intake @36.8psia, vacuum from 20.0 to 24.0psi @13:43:59.80 SS.reconfigure forSampling @13:43:59.84 SS.seek 25.00ml @13:44:01.82 Vacuum too high -- waiting up to 40s @13:44:02.08 Intake @36.8psia, vacuum from 20.0 to 24.0psi @13:44:02.09 SS.reconfigure forSampling @13:44:02.13 SS.seek 25.00ml @13:44:03.48 Vacuum too high -- waiting up to 40s @13:44:03.74 Intake @36.9psia, vacuum from 20.0 to 24.0psi @13:44:03.75 SS.reconfigure forSampling @13:44:03.79 SS.seek 25.00ml @13:44:05.23 Vacuum too high -- waiting up to 40s @13:44:05.49 Intake @37.3psia, vacuum from 20.0 to 24.0psi @13:44:05.50 SS.reconfigure forSampling @13:44:05.54 SS.seek 25.00ml @13:44:06.91 Vacuum too high -- waiting up to 40s @13:44:07.17 Intake @37.6psia, vacuum from 20.0 to 24.0psi @13:44:07.18 SS.reconfigure forSampling @13:44:07.21 SS.seek 25.00ml @13:44:08.35 Vacuum too high -- waiting up to 40s @13:44:08.62 Intake @36.8psia, vacuum from 20.0 to 24.0psi @13:44:08.63 SS.reconfigure forSampling @13:44:08.66 SS.seek 25.00ml @13:44:10.07 Vacuum too high -- waiting up to 40s @13:44:10.33 Intake @37.0psia, vacuum from 20.0 to 24.0psi @13:44:10.34 SS.reconfigure forSampling @13:44:10.38 SS.seek 25.00ml @13:44:11.60 Vacuum too high -- waiting up to 40s @13:44:11.86 Intake @37.1psia, vacuum from 20.0 to 24.0psi @13:44:11.87 SS.reconfigure forSampling @13:44:11.91 SS.seek 25.00ml @13:44:13.16 Vacuum too high -- waiting up to 40s @13:44:13.43 Intake @37.0psia, vacuum from 20.0 to 24.0psi @13:44:13.44 SS.reconfigure forSampling @13:44:13.47 SS.seek 25.00ml @13:44:14.66 Vacuum too high -- waiting up to 40s @13:44:14.92 Intake @36.7psia, vacuum from 20.0 to 24.0psi @13:44:14.93 SS.reconfigure forSampling @13:44:14.97 SS.seek 25.00ml @13:44:16.54 Vacuum too high -- waiting up to 40s @13:44:16.80 Intake @37.4psia, vacuum from 20.0 to 24.0psi @13:44:16.81 SS.reconfigure forSampling @13:44:16.84 SS.seek 25.00ml @13:44:18.04 Vacuum too high -- waiting up to 40s @13:44:18.30 Intake @37.4psia, vacuum from 20.0 to 24.0psi @13:44:18.31 SS.reconfigure forSampling @13:44:18.34 SS.seek 25.00ml @13:44:19.48 Vacuum too high -- waiting up to 40s @13:44:19.74 Intake @36.5psia, vacuum from 20.0 to 24.0psi @13:44:19.75 SS.reconfigure forSampling @13:44:19.79 SS.seek 25.00ml @13:44:21.48 Vacuum too high -- waiting up to 40s @13:44:21.74 Intake @37.0psia, vacuum from 20.0 to 24.0psi @13:44:21.75 SS.reconfigure forSampling @13:44:21.79 SS.seek 25.00ml @13:44:23.07 Vacuum too high -- waiting up to 40s @13:44:23.33 Intake @36.8psia, vacuum from 20.0 to 24.0psi @13:44:23.34 SS.reconfigure forSampling @13:44:23.38 SS.seek 25.00ml @13:44:24.69 Vacuum too high -- waiting up to 40s @13:44:24.95 Intake @37.1psia, vacuum from 20.0 to 24.0psi @13:44:24.96 SS.reconfigure forSampling @13:44:25.00 SS.seek 25.00ml @13:44:26.26 Vacuum too high -- waiting up to 40s @13:44:26.52 Intake @37.3psia, vacuum from 20.0 to 24.0psi @13:44:26.53 SS.reconfigure forSampling @13:44:26.56 SS.seek 25.00ml @13:44:27.88 Vacuum too high -- waiting up to 40s @13:44:28.14 SS.seek 25.00ml @13:44:29.26 Vacuum too high -- waiting up to 40s @13:44:29.52 Intake @37.0psia, vacuum from 20.0 to 24.0psi @13:44:29.53 SS.reconfigure forSampling @13:44:29.56 SS.seek 25.00ml @13:44:30.91 Vacuum too high -- waiting up to 40s @13:44:31.18 SS.seek 25.00ml @13:44:32.19 Vacuum too high -- waiting up to 40s @13:44:32.45 Intake @37.0psia, vacuum from 20.0 to 24.0psi @13:44:32.46 SS.reconfigure forSampling @13:44:32.50 SS.seek 25.00ml @13:44:33.63 Vacuum too high -- waiting up to 40s @13:44:33.89 Intake @36.6psia, vacuum from 20.0 to 24.0psi @13:44:33.90 SS.reconfigure forSampling @13:44:33.93 SS.seek 25.00ml @13:44:35.19 Vacuum too high -- waiting up to 40s @13:44:35.45 Intake @36.8psia, vacuum from 20.0 to 24.0psi @13:44:35.46 SS.reconfigure forSampling @13:44:35.49 SS.seek 25.00ml @13:44:36.55 Stroke completed -- waiting for pressure to equalize ... @13:44:38.96 Intake.select :close @13:44:39.09 SSV.dial :exhaust @13:44:39.88 Exhaust.select :open @13:44:39.97 SS.reconfigure SSexhale @13:44:40.50 SS.seek 0.00ml @13:44:49.30 Exhaust.select :close @13:44:49.39 SSV.dial CBV @13:44:49.41
CBV.dial SSV @13:44:50.19 Allowing 185s for 25.0ml of remaining 350.0ml @13:44:50.20 Intake.select :open @13:44:52.33 Intake @36.8psia, vacuum from 20.0 to 24.0psi @13:44:52.34 SS.reconfigure forSampling @13:44:52.38 SS.seek 25.00ml @13:44:54.97 Vacuum too high -- waiting up to 40s @13:44:55.23 Intake @37.0psia, vacuum from 20.0 to 24.0psi @13:44:55.24 SS.reconfigure forSampling @13:44:55.28 SS.seek 25.00ml @13:44:56.72 Vacuum too high -- waiting up to 40s @13:44:56.99 SS.seek 25.00ml @13:44:58.47 Vacuum too high -- waiting up to 40s @13:44:58.73 Intake @37.1psia, vacuum from 20.0 to 24.0psi @13:44:58.74 SS.reconfigure forSampling @13:44:58.77 SS.seek 25.00ml @13:45:00.22 Vacuum too high -- waiting up to 40s @13:45:00.48 Intake @37.3psia, vacuum from 20.0 to 24.0psi @13:45:00.49 SS.reconfigure forSampling @13:45:00.52 SS.seek 25.00ml @13:45:01.88 Vacuum too high -- waiting up to 40s @13:45:02.14 Intake @37.5psia, vacuum from 20.0 to 24.0psi @13:45:02.15 SS.reconfigure forSampling @13:45:02.19 SS.seek 25.00ml @13:45:03.60 Vacuum too high -- waiting up to 40s @13:45:03.86 Intake @37.4psia, vacuum from 20.0 to 24.0psi @13:45:03.87 SS.reconfigure forSampling @13:45:03.90 SS.seek 25.00ml @13:45:05.19 Vacuum too high -- waiting up to 40s @13:45:05.45 Intake @36.7psia, vacuum from 20.0 to 24.0psi @13:45:05.46 SS.reconfigure forSampling @13:45:05.50 SS.seek 25.00ml @13:45:06.88 Vacuum too high -- waiting up to 40s @13:45:07.14 Intake @37.0psia, vacuum from 20.0 to 24.0psi @13:45:07.15 SS.reconfigure forSampling @13:45:07.18 SS.seek 25.00ml @13:45:08.66 Vacuum too high -- waiting up to 40s @13:45:08.92 Intake @37.0psia, vacuum from 20.0 to 24.0psi @13:45:08.93 SS.reconfigure forSampling @13:45:08.96 SS.seek 25.00ml @13:45:10.32 Vacuum too high -- waiting up to 40s @13:45:10.58 Intake @37.2psia, vacuum from 20.0 to 24.0psi @13:45:10.59 SS.reconfigure forSampling @13:45:10.62 SS.seek 25.00ml @13:45:12.10 Vacuum too high -- waiting up to 40s @13:45:12.37 Intake @37.2psia, vacuum from 20.0 to 24.0psi @13:45:12.38 SS.reconfigure forSampling @13:45:12.41 SS.seek 25.00ml @13:45:13.75 Vacuum too high -- waiting up to 40s @13:45:14.01 Intake @37.0psia, vacuum from 20.0 to 24.0psi @13:45:14.02 SS.reconfigure forSampling @13:45:14.05 SS.seek 25.00ml @13:45:15.47 Vacuum too high -- waiting up to 40s @13:45:15.73 Intake @37.2psia, vacuum from 20.0 to 24.0psi @13:45:15.74 SS.reconfigure forSampling @13:45:15.77 SS.seek 25.00ml @13:45:17.16 Vacuum too high -- waiting up to 40s @13:45:17.42 Intake @37.2psia, vacuum from 20.0 to 24.0psi @13:45:17.43 SS.reconfigure forSampling @13:45:17.47 SS.seek 25.00ml @13:45:18.78 Vacuum too high -- waiting up to 40s @13:45:19.04 Intake @37.0psia, vacuum from 20.0 to 24.0psi @13:45:19.05 SS.reconfigure forSampling @13:45:19.08 SS.seek 25.00ml @13:45:20.47 Vacuum too high -- waiting up to 40s @13:45:20.74 SS.seek 25.00ml @13:45:21.97 Vacuum too high -- waiting up to 40s @13:45:22.23 Intake @36.8psia, vacuum from 20.0 to 24.0psi @13:45:22.24 SS.reconfigure forSampling @13:45:22.27 SS.seek 25.00ml @13:45:23.72 Vacuum too high -- waiting up to 40s @13:45:23.98 Intake @37.5psia, vacuum from 20.0 to 24.0psi @13:45:23.99 SS.reconfigure forSampling @13:45:24.02 SS.seek 25.00ml @13:45:25.38 Vacuum too high -- waiting up to 40s @13:45:25.64 Intake @37.0psia, vacuum from 20.0 to 24.0psi @13:45:25.65 SS.reconfigure forSampling @13:45:25.68 SS.seek 25.00ml @13:45:27.03 Vacuum too high -- waiting up to 40s @13:45:27.29 Intake @36.8psia, vacuum from 20.0 to 24.0psi @13:45:27.30 SS.reconfigure forSampling @13:45:27.33 SS.seek 25.00ml @13:45:28.66 Vacuum too high -- waiting up to 40s @13:45:28.92 Intake @36.9psia, vacuum from 20.0 to 24.0psi @13:45:28.93 SS.reconfigure forSampling @13:45:28.96 SS.seek 25.00ml @13:45:30.44 Vacuum too high -- waiting up to 40s @13:45:30.70 Intake @36.8psia, vacuum from 20.0 to 24.0psi @13:45:30.71 SS.reconfigure forSampling @13:45:30.74 SS.seek 25.00ml @13:45:31.94 Vacuum too high -- waiting up to 40s @13:45:32.20 SS.seek 25.00ml @13:45:33.41 Vacuum too high -- waiting up to 40s @13:45:33.67 Intake @36.9psia, vacuum from 20.0 to 24.0psi @13:45:34.13 SS.reconfigure forSampling @13:45:34.17 SS.seek 25.00ml @13:45:36.10 Vacuum too high -- waiting up to 40s @13:45:36.36 Intake @37.3psia, vacuum from 20.0 to 24.0psi @13:45:36.37 SS.reconfigure forSampling @13:45:36.41 SS.seek 25.00ml @13:45:37.53 Vacuum too high -- waiting up to 40s @13:45:37.79 Intake @36.5psia, vacuum from 20.0 to 24.0psi @13:45:37.80 SS.reconfigure forSampling @13:45:37.84 SS.seek 25.00ml @13:45:39.16 Vacuum too high -- waiting up to 40s @13:45:39.42 Intake @36.8psia, vacuum from 20.0 to 24.0psi @13:45:39.43 SS.reconfigure forSampling @13:45:39.47 SS.seek 25.00ml @13:45:40.94 Vacuum too high -- waiting up to 40s @13:45:41.20 Intake @37.0psia, vacuum from 20.0 to 24.0psi @13:45:41.21 SS.reconfigure forSampling @13:45:41.25 SS.seek 25.00ml @13:45:42.44 Vacuum too high -- waiting up to 40s @13:45:42.70 Intake @37.3psia, vacuum from 20.0 to 24.0psi @13:45:42.71 SS.reconfigure forSampling @13:45:42.75 SS.seek 25.00ml @13:45:43.97 Vacuum too high -- waiting up to 40s @13:45:44.24 Intake @36.9psia, vacuum from 20.0 to 24.0psi @13:45:44.25 SS.reconfigure forSampling @13:45:44.28 SS.seek 25.00ml @13:45:45.53 Vacuum too high -- waiting up to 40s @13:45:45.79 Intake @37.1psia, vacuum from 20.0 to 24.0psi @13:45:45.80 SS.reconfigure forSampling @13:45:45.84 SS.seek 25.00ml @13:45:46.97 Vacuum too high -- waiting up to 40s @13:45:47.23 Intake @37.4psia, vacuum from 20.0 to 24.0psi @13:45:47.24 SS.reconfigure forSampling @13:45:47.28 SS.seek 25.00ml @13:45:48.53 Vacuum too high -- waiting up to 40s @13:45:48.79 Intake @37.2psia, vacuum from 20.0 to 24.0psi @13:45:48.80 SS.reconfigure forSampling @13:45:48.83 SS.seek 25.00ml @13:45:49.88 Vacuum too high -- waiting up to 40s @13:45:50.14 Intake @36.7psia, vacuum from 20.0 to 24.0psi @13:45:50.15 SS.reconfigure forSampling @13:45:50.19 SS.seek 25.00ml @13:45:51.60 Vacuum too high -- waiting up to 40s @13:45:51.86 Intake @36.8psia, vacuum from 20.0 to 24.0psi @13:45:51.87 SS.reconfigure forSampling @13:45:51.91 SS.seek 25.00ml @13:45:53.06 Vacuum too high -- waiting up to 40s @13:45:53.32 Intake @37.2psia, vacuum from 20.0 to 24.0psi @13:45:53.33 SS.reconfigure forSampling @13:45:53.36 SS.seek 25.00ml @13:45:54.47 Vacuum too high -- waiting up to 40s @13:45:54.73 Intake @37.2psia, vacuum from 20.0 to 24.0psi @13:45:54.74 SS.reconfigure forSampling @13:45:54.77 SS.seek 25.00ml @13:45:56.00 Vacuum too high -- waiting up to 40s @13:45:56.26 Intake @37.1psia, vacuum from 20.0 to 24.0psi @13:45:56.27 SS.reconfigure forSampling @13:45:56.30 SS.seek 25.00ml @13:45:57.53 Vacuum too high -- waiting up to 40s @13:45:57.79 Intake @37.0psia, vacuum from 20.0 to 24.0psi @13:45:57.80 SS.reconfigure forSampling @13:45:57.83 SS.seek 25.00ml @13:45:59.03 Vacuum too high -- waiting up to 40s @13:45:59.29 Intake @37.2psia, vacuum from 20.0 to 24.0psi @13:45:59.30 SS.reconfigure forSampling @13:45:59.34 SS.seek 25.00ml @13:46:00.44 Vacuum too high -- waiting up to 40s @13:46:00.70 Intake @37.2psia, vacuum from 20.0 to 24.0psi @13:46:00.71 SS.reconfigure forSampling @13:46:00.74 SS.seek 25.00ml @13:46:01.88 Vacuum too high -- waiting up to 40s @13:46:02.14 Intake @37.0psia, vacuum from 20.0 to 24.0psi @13:46:02.15 SS.reconfigure forSampling @13:46:02.18 SS.seek 25.00ml @13:46:03.38 Vacuum too high -- waiting up to 40s @13:46:03.64 Intake @37.1psia, vacuum from 20.0 to 24.0psi @13:46:03.65 SS.reconfigure forSampling @13:46:03.68 SS.seek 25.00ml @13:46:04.91 Vacuum too high -- waiting up to 40s @13:46:05.17 Intake @37.3psia, vacuum from 20.0 to 24.0psi @13:46:05.18 SS.reconfigure forSampling @13:46:05.21 SS.seek 25.00ml @13:46:06.34 Vacuum too high -- waiting up to 40s @13:46:06.60 Intake @37.2psia, vacuum from 20.0 to 24.0psi @13:46:06.61 SS.reconfigure forSampling @13:46:06.65 SS.seek 25.00ml @13:46:07.88 Vacuum too high -- waiting up to 40s @13:46:08.14 Intake @37.1psia, vacuum from 20.0 to 24.0psi @13:46:08.15 SS.reconfigure forSampling @13:46:08.18 SS.seek 25.00ml @13:46:09.25 Vacuum too high -- waiting up to 40s @13:46:09.51 Intake @36.8psia, vacuum from 20.0 to 24.0psi @13:46:09.52 SS.reconfigure forSampling @13:46:09.55 SS.seek 25.00ml @13:46:10.63 Vacuum too high -- waiting up to 40s @13:46:10.89 Intake @37.0psia, vacuum from 20.0 to 24.0psi @13:46:10.90 SS.reconfigure forSampling @13:46:10.93 SS.seek 25.00ml @13:46:12.06 Vacuum too high -- waiting up to 40s @13:46:12.81 Intake @37.5psia, vacuum from 20.0 to 24.0psi @13:46:12.83 SS.reconfigure forSampling @13:46:12.86 SS.seek 25.00ml @13:46:14.56 Vacuum too high -- waiting up to 40s @13:46:14.82 Intake @37.2psia, vacuum from 20.0 to 24.0psi @13:46:14.83 SS.reconfigure forSampling @13:46:14.87 SS.seek 25.00ml @13:46:15.94 Vacuum too high -- waiting up to 40s @13:46:16.20 Intake @37.1psia, vacuum from 20.0 to 24.0psi @13:46:16.21 SS.reconfigure forSampling @13:46:16.25 SS.seek 25.00ml @13:46:17.31 Vacuum too high -- waiting up to 40s @13:46:17.57 Intake @37.0psia, vacuum from 20.0 to 24.0psi @13:46:17.58 SS.reconfigure forSampling @13:46:17.62 SS.seek 25.00ml @13:46:18.72 Vacuum too high -- waiting up to 40s @13:46:18.98 Intake @36.6psia, vacuum from 20.0 to 24.0psi @13:46:18.99 SS.reconfigure forSampling @13:46:19.03 SS.seek 25.00ml @13:46:20.13 Vacuum too high -- waiting up to 40s @13:46:20.39 Intake @37.1psia, vacuum from 20.0 to 24.0psi @13:46:20.40 SS.reconfigure forSampling @13:46:20.44 SS.seek 25.00ml @13:46:21.59 Vacuum too high -- waiting up to 40s @13:46:21.85 Intake @37.4psia, vacuum from 20.0 to 24.0psi @13:46:21.86 SS.reconfigure forSampling @13:46:21.90 SS.seek 25.00ml @13:46:22.88 Vacuum too high -- waiting up to 40s @13:46:23.14 Intake @37.0psia, vacuum from 20.0 to 24.0psi @13:46:23.15 SS.reconfigure forSampling @13:46:23.19 SS.seek 25.00ml @13:46:24.25 Vacuum too high -- waiting up to 40s @13:46:24.51 Intake @36.7psia, vacuum from 20.0 to 24.0psi @13:46:24.52 SS.reconfigure forSampling @13:46:24.55 SS.seek 25.00ml @13:46:25.66 Vacuum too high -- waiting up to 40s @13:46:25.92 Intake @37.0psia, vacuum from 20.0 to 24.0psi @13:46:25.93 SS.reconfigure forSampling @13:46:25.96 SS.seek 25.00ml @13:46:26.97 Vacuum too high -- waiting up to 40s @13:46:27.23 Intake @36.8psia, vacuum from 20.0 to 24.0psi @13:46:27.24 SS.reconfigure forSampling @13:46:27.28 SS.seek 25.00ml @13:46:28.47 Vacuum too high -- waiting up to 40s @13:46:28.73 Intake @37.2psia, vacuum from 20.0 to 24.0psi @13:46:28.74 SS.reconfigure forSampling @13:46:28.77 SS.seek 25.00ml @13:46:29.87 Vacuum too high -- waiting up to 40s @13:46:30.13 Intake @37.4psia, vacuum from 20.0 to 24.0psi @13:46:30.14 SS.reconfigure forSampling @13:46:30.17 SS.seek 25.00ml @13:46:31.12 Vacuum too high -- waiting up to 40s @13:46:31.38 Intake @37.0psia, vacuum from 20.0 to 24.0psi @13:46:31.39 SS.reconfigure forSampling @13:46:31.42 SS.seek 25.00ml @13:46:32.41 Vacuum too high -- waiting up to 40s @13:46:32.67 Intake @36.9psia, vacuum from 20.0 to 24.0psi @13:46:32.68 SS.reconfigure forSampling @13:46:32.71 SS.seek 25.00ml @13:46:33.75 Vacuum too high -- waiting up to 40s @13:46:34.01 Intake @37.1psia, vacuum from 20.0 to 24.0psi @13:46:34.02 SS.reconfigure forSampling @13:46:34.05 SS.seek 25.00ml @13:46:35.09 Vacuum too high -- waiting up to 40s @13:46:35.35 Intake @37.0psia, vacuum from 20.0 to 24.0psi @13:46:35.36 SS.reconfigure forSampling @13:46:35.39 SS.seek 25.00ml @13:46:36.47 Vacuum too high -- waiting up to 40s @13:46:36.73 Intake @37.1psia, vacuum from 20.0 to 24.0psi @13:46:36.74 SS.reconfigure forSampling @13:46:36.77 SS.seek 25.00ml @13:46:37.72 Vacuum too high -- waiting up to 40s @13:46:37.98 Intake @37.0psia, vacuum from 20.0 to 24.0psi @13:46:37.99 SS.reconfigure forSampling @13:46:38.02 SS.seek 25.00ml @13:46:39.06 Vacuum too high -- waiting up to 40s @13:46:39.32 Intake @37.0psia, vacuum from 20.0 to 24.0psi @13:46:39.33 SS.reconfigure forSampling @13:46:39.36 SS.seek 25.00ml @13:46:40.44 Vacuum too high -- waiting up to 40s @13:46:40.70 Intake @37.1psia, vacuum from 20.0 to 24.0psi @13:46:40.71 SS.reconfigure forSampling @13:46:40.74 SS.seek 25.00ml @13:46:41.81 Vacuum too high -- waiting up to 40s @13:46:42.07 Intake @36.9psia, vacuum from 20.0 to 24.0psi @13:46:42.08 SS.reconfigure forSampling @13:46:42.11 SS.seek 25.00ml @13:46:43.19 Vacuum too high -- waiting up to 40s @13:46:43.46 SS.seek 25.00ml @13:46:44.50 Vacuum too high -- waiting up to 40s @13:46:44.76 Intake @37.0psia, vacuum from 20.0 to 24.0psi @13:46:44.77 SS.reconfigure forSampling @13:46:44.80 SS.seek 25.00ml @13:46:45.81 Vacuum too high -- waiting up to 40s @13:46:46.07 Intake @36.9psia, vacuum from 20.0 to 24.0psi @13:46:46.08 SS.reconfigure forSampling @13:46:46.11 SS.seek 25.00ml @13:46:47.73 Vacuum too high -- waiting up to 40s @13:46:47.99 Intake @37.4psia, vacuum from 20.0 to 24.0psi @13:46:48.00 SS.reconfigure forSampling @13:46:48.04 SS.seek 25.00ml @13:46:49.72 Vacuum too high -- waiting up to 40s @13:46:49.98 Intake @37.4psia, vacuum from 20.0 to 24.0psi @13:46:49.99 SS.reconfigure forSampling @13:46:50.03 SS.seek 25.00ml @13:46:51.06 Vacuum too high -- waiting up to 40s @13:46:51.32 Intake @37.2psia, vacuum from 20.0 to 24.0psi @13:46:51.34 SS.reconfigure forSampling @13:46:51.37 SS.seek 25.00ml @13:46:52.44 Vacuum too high -- waiting up to 40s @13:46:52.70 Intake @37.0psia, vacuum from 20.0 to 24.0psi @13:46:52.71 SS.reconfigure forSampling @13:46:52.75 SS.seek 25.00ml @13:46:53.87 Vacuum too high -- waiting up to 40s @13:46:54.36 Intake @36.9psia, vacuum from 20.0 to 24.0psi @13:46:54.38 SS.reconfigure forSampling @13:46:54.41 SS.seek 25.00ml @13:46:55.69 Vacuum too high -- waiting up to 40s @13:46:55.95 Intake @36.8psia, vacuum from 20.0 to 24.0psi @13:46:55.96 SS.reconfigure forSampling @13:46:56.00 SS.seek 25.00ml @13:46:57.12 Vacuum too high -- waiting up to 40s @13:46:57.38 Intake @37.1psia, vacuum from 20.0 to 24.0psi @13:46:57.39 SS.reconfigure forSampling @13:46:57.43 SS.seek 25.00ml @13:46:58.59 Vacuum too high -- waiting up to 40s @13:46:58.85 Intake @37.4psia, vacuum from 20.0 to 24.0psi @13:46:58.86 SS.reconfigure forSampling @13:46:58.90 SS.seek 25.00ml @13:46:59.87 Vacuum too high -- waiting up to 40s @13:47:00.13 Intake @37.1psia, vacuum from 20.0 to 24.0psi @13:47:00.14 SS.reconfigure forSampling @13:47:00.18 SS.seek 25.00ml @13:47:01.19 Vacuum too high -- waiting up to 40s @13:47:01.45 Intake @37.0psia, vacuum from 20.0 to 24.0psi @13:47:01.46 SS.reconfigure forSampling @13:47:01.49 SS.seek 25.00ml @13:47:02.56 Vacuum too high -- waiting up to 40s @13:47:02.82 Intake @37.2psia, vacuum from 20.0 to 24.0psi @13:47:02.83 SS.reconfigure forSampling @13:47:02.86 SS.seek 25.00ml @13:47:03.90 Vacuum too high -- waiting up to 40s @13:47:04.17 Intake @37.1psia, vacuum from 20.0 to 24.0psi @13:47:04.18 SS.reconfigure forSampling @13:47:04.21 SS.seek 25.00ml @13:47:05.19 Vacuum too high -- waiting up to 40s @13:47:05.45 Intake @37.2psia, vacuum from 20.0 to 24.0psi @13:47:05.46 SS.reconfigure forSampling @13:47:05.49 SS.seek 25.00ml @13:47:06.44 Vacuum too high -- waiting up to 40s @13:47:06.70 Intake @37.2psia, vacuum from 20.0 to 24.0psi @13:47:06.71 SS.reconfigure forSampling @13:47:06.75 SS.seek 25.00ml @13:47:07.75 Vacuum too high -- waiting up to 40s @13:47:08.01 Intake @37.1psia, vacuum from 20.0 to 24.0psi @13:47:08.02 SS.reconfigure forSampling @13:47:08.06 SS.seek 25.00ml @13:47:09.12 Vacuum too high -- waiting up to 40s @13:47:09.38 Intake @37.0psia, vacuum from 20.0 to 24.0psi @13:47:09.39 SS.reconfigure forSampling @13:47:09.42 SS.seek 25.00ml @13:47:10.56 Vacuum too high -- waiting up to 40s @13:47:10.82 Intake @37.4psia, vacuum from 20.0 to 24.0psi @13:47:10.83 SS.reconfigure forSampling @13:47:10.86 SS.seek 25.00ml @13:47:11.94 Vacuum too high -- waiting up to 40s @13:47:12.20 Intake @37.0psia, vacuum from 20.0 to 24.0psi @13:47:12.21 SS.reconfigure forSampling @13:47:12.24 SS.seek 25.00ml @13:47:13.25 Vacuum too high -- waiting up to 40s @13:47:13.51 Intake @36.8psia, vacuum from 20.0 to 24.0psi @13:47:13.52 SS.reconfigure forSampling @13:47:13.55 SS.seek 25.00ml @13:47:14.56 Vacuum too high -- waiting up to 40s @13:47:14.82 Intake @36.8psia, vacuum from 20.0 to 24.0psi @13:47:14.83 SS.reconfigure forSampling @13:47:14.86 SS.seek 25.00ml @13:47:15.97 Vacuum too high -- waiting up to 40s @13:47:16.23 Intake @37.7psia, vacuum from 20.0 to 24.0psi @13:47:16.24 SS.reconfigure forSampling @13:47:16.27 SS.seek 25.00ml @13:47:17.28 Vacuum too high -- waiting up to 40s @13:47:17.54 Intake @37.3psia, vacuum from 20.0 to 24.0psi @13:47:17.55 SS.reconfigure forSampling @13:47:17.58 SS.seek 25.00ml @13:47:18.53 Vacuum too high -- waiting up to 40s @13:47:18.80 Intake @36.6psia, vacuum from 20.0 to 24.0psi @13:47:18.81 SS.reconfigure forSampling @13:47:18.84 SS.seek 25.00ml @13:47:19.90 Vacuum too high -- waiting up to 40s @13:47:20.16 Intake @36.8psia, vacuum from 20.0 to 24.0psi @13:47:20.17 SS.reconfigure forSampling @13:47:20.20 SS.seek 25.00ml @13:47:21.25 Vacuum too high -- waiting up to 40s @13:47:21.98 Intake @37.1psia, vacuum from 20.0 to 24.0psi @13:47:21.99 SS.reconfigure forSampling @13:47:22.02 SS.seek 25.00ml @13:47:23.53 Vacuum too high -- waiting up to 40s @13:47:23.79 Intake @37.0psia, vacuum from 20.0 to 24.0psi @13:47:23.80 SS.reconfigure forSampling @13:47:23.84 SS.seek 25.00ml @13:47:24.84 Vacuum too high -- waiting up to 40s @13:47:25.10 Intake @36.8psia, vacuum from 20.0 to 24.0psi @13:47:25.12 SS.reconfigure forSampling @13:47:25.15 SS.seek 25.00ml @13:47:26.25 Vacuum too high -- waiting up to 40s @13:47:26.51 Intake @37.5psia, vacuum from 20.0 to 24.0psi @13:47:26.53 SS.reconfigure forSampling @13:47:26.56 SS.seek 25.00ml @13:47:27.68 Vacuum too high -- waiting up to 40s @13:47:27.94 Intake @37.4psia, vacuum from 20.0 to 24.0psi @13:47:27.95 SS.reconfigure forSampling @13:47:27.99 SS.seek 25.00ml @13:47:28.87 Vacuum too high -- waiting up to 40s @13:47:29.13 Intake @36.8psia, vacuum from 20.0 to 24.0psi @13:47:29.14 SS.reconfigure forSampling @13:47:29.18 SS.seek 25.00ml @13:47:30.25 Vacuum too high -- waiting up to 40s @13:47:30.62 Intake @37.3psia, vacuum from 20.0 to 24.0psi @13:47:30.63 SS.reconfigure forSampling @13:47:30.66 SS.seek 25.00ml @13:47:31.84 Vacuum too high -- waiting up to 40s @13:47:32.10 Intake @37.2psia, vacuum from 20.0 to 24.0psi @13:47:32.11 SS.reconfigure forSampling @13:47:32.15 SS.seek 25.00ml @13:47:33.15 Vacuum too high -- waiting up to 40s @13:47:33.41 Intake @37.1psia, vacuum from 20.0 to 24.0psi @13:47:33.42 SS.reconfigure forSampling @13:47:33.46 SS.seek 25.00ml @13:47:34.43 Vacuum too high -- waiting up to 40s @13:47:34.69 Intake @37.0psia, vacuum from 20.0 to 24.0psi @13:47:34.70 SS.reconfigure forSampling @13:47:34.73 SS.seek 25.00ml @13:47:35.68 Vacuum too high -- waiting up to 40s @13:47:35.94 Intake @37.2psia, vacuum from 20.0 to 24.0psi @13:47:35.95 SS.reconfigure forSampling @13:47:35.98 SS.seek 25.00ml @13:47:37.06 Vacuum too high -- waiting up to 40s @13:47:37.33 SS.seek 25.00ml @13:47:38.19 Stroke completed -- waiting for pressure to equalize ... @13:47:40.58 Intake.select :close @13:47:40.72 SSV.dial :exhaust @13:47:41.51 Exhaust.select :open @13:47:41.60 SS.reconfigure SSexhale @13:47:41.64 SS.seek 0.00ml @13:47:50.47 Exhaust.select :close @13:47:50.56 SSV.dial CBV @13:47:50.58
CBV.dial SSV @13:47:51.37 Allowing 185s for 25.0ml of remaining 325.0ml @13:47:51.38 Intake.select :open @13:47:53.51 Intake @37.9psia, vacuum from 20.0 to 24.0psi @13:47:53.52 SS.reconfigure forSampling @13:47:53.56 SS.seek 25.00ml @13:47:55.68 Vacuum too high -- waiting up to 40s @13:47:55.94 Intake @37.2psia, vacuum from 20.0 to 24.0psi @13:47:55.95 SS.reconfigure forSampling @13:47:55.98 SS.seek 25.00ml @13:47:57.18 Vacuum too high -- waiting up to 40s @13:47:57.44 Intake @36.9psia, vacuum from 20.0 to 24.0psi @13:47:57.45 SS.reconfigure forSampling @13:47:57.48 SS.seek 25.00ml @13:47:58.53 Vacuum too high -- waiting up to 40s @13:47:58.79 Intake @37.0psia, vacuum from 20.0 to 24.0psi @13:47:58.80 SS.reconfigure forSampling @13:47:58.83 SS.seek 25.00ml @13:48:00.00 Vacuum too high -- waiting up to 40s @13:48:00.26 Intake @37.1psia, vacuum from 20.0 to 24.0psi @13:48:00.27 SS.reconfigure forSampling @13:48:00.30 SS.seek 25.00ml @13:48:01.56 Vacuum too high -- waiting up to 40s @13:48:01.82 Intake @37.4psia, vacuum from 20.0 to 24.0psi @13:48:01.83 SS.reconfigure forSampling @13:48:01.87 SS.seek 25.00ml @13:48:03.06 Vacuum too high -- waiting up to 40s @13:48:03.33 Intake @37.6psia, vacuum from 20.0 to 24.0psi @13:48:03.34 SS.reconfigure forSampling @13:48:03.37 SS.seek 25.00ml @13:48:04.53 Vacuum too high -- waiting up to 40s @13:48:04.79 Intake @37.4psia, vacuum from 20.0 to 24.0psi @13:48:04.80 SS.reconfigure forSampling @13:48:04.83 SS.seek 25.00ml @13:48:05.93 Vacuum too high -- waiting up to 40s @13:48:06.19 Intake @37.5psia, vacuum from 20.0 to 24.0psi @13:48:06.20 SS.reconfigure forSampling @13:48:06.23 SS.seek 25.00ml @13:48:07.31 Vacuum too high -- waiting up to 40s @13:48:07.57 Intake @37.4psia, vacuum from 20.0 to 24.0psi @13:48:07.58 SS.reconfigure forSampling @13:48:07.62 SS.seek 25.00ml @13:48:08.78 Vacuum too high -- waiting up to 40s @13:48:09.56 Intake @37.1psia, vacuum from 20.0 to 24.0psi @13:48:09.57 SS.reconfigure forSampling @13:48:09.61 SS.seek 25.00ml @13:48:11.34 Vacuum too high -- waiting up to 40s @13:48:11.60 Intake @37.1psia, vacuum from 20.0 to 24.0psi @13:48:11.61 SS.reconfigure forSampling @13:48:11.65 SS.seek 25.00ml @13:48:12.68 Vacuum too high -- waiting up to 40s @13:48:12.94 Intake @36.9psia, vacuum from 20.0 to 24.0psi @13:48:12.95 SS.reconfigure forSampling @13:48:12.99 SS.seek 25.00ml @13:48:14.06 Vacuum too high -- waiting up to 40s @13:48:14.32 Intake @37.3psia, vacuum from 20.0 to 24.0psi @13:48:14.33 SS.reconfigure forSampling @13:48:14.37 SS.seek 25.00ml @13:48:15.59 Vacuum too high -- waiting up to 40s @13:48:15.85 Intake @38.0psia, vacuum from 20.0 to 24.0psi @13:48:15.86 SS.reconfigure forSampling @13:48:15.90 SS.seek 25.00ml @13:48:16.90 Vacuum too high -- waiting up to 40s @13:48:17.16 Intake @37.0psia, vacuum from 20.0 to 24.0psi @13:48:17.17 SS.reconfigure forSampling @13:48:17.21 SS.seek 25.00ml @13:48:18.40 Vacuum too high -- waiting up to 40s @13:48:18.66 Intake @37.0psia, vacuum from 20.0 to 24.0psi @13:48:18.67 SS.reconfigure forSampling @13:48:18.71 SS.seek 25.00ml @13:48:19.81 Vacuum too high -- waiting up to 40s @13:48:20.08 SS.seek 25.00ml @13:48:21.09 Vacuum too high -- waiting up to 40s @13:48:21.35 Intake @36.5psia, vacuum from 20.0 to 24.0psi @13:48:21.36 SS.reconfigure forSampling @13:48:21.40 SS.seek 25.00ml @13:48:22.53 Vacuum too high -- waiting up to 40s @13:48:22.79 Intake @36.7psia, vacuum from 20.0 to 24.0psi @13:48:22.80 SS.reconfigure forSampling @13:48:22.84 SS.seek 25.00ml @13:48:24.21 Vacuum too high -- waiting up to 40s @13:48:24.69 Intake @37.7psia, vacuum from 20.0 to 24.0psi @13:48:24.70 SS.reconfigure forSampling @13:48:24.73 SS.seek 25.00ml @13:48:26.06 Vacuum too high -- waiting up to 40s @13:48:26.32 Intake @36.9psia, vacuum from 20.0 to 24.0psi @13:48:26.33 SS.reconfigure forSampling @13:48:26.36 SS.seek 25.00ml @13:48:27.40 Vacuum too high -- waiting up to 40s @13:48:27.66 Intake @36.6psia, vacuum from 20.0 to 24.0psi @13:48:27.67 SS.reconfigure forSampling @13:48:27.71 SS.seek 25.00ml @13:48:28.81 Vacuum too high -- waiting up to 40s @13:48:29.30 Intake @37.0psia, vacuum from 20.0 to 24.0psi @13:48:29.31 SS.reconfigure forSampling @13:48:29.34 SS.seek 25.00ml @13:48:30.77 Vacuum too high -- waiting up to 40s @13:48:31.04 Intake @37.2psia, vacuum from 20.0 to 24.0psi @13:48:31.05 SS.reconfigure forSampling @13:48:31.08 SS.seek 25.00ml @13:48:32.12 Vacuum too high -- waiting up to 40s @13:48:32.38 Intake @37.2psia, vacuum from 20.0 to 24.0psi @13:48:32.39 SS.reconfigure forSampling @13:48:32.42 SS.seek 25.00ml @13:48:33.46 Vacuum too high -- waiting up to 40s @13:48:33.72 Intake @37.1psia, vacuum from 20.0 to 24.0psi @13:48:33.73 SS.reconfigure forSampling @13:48:33.76 SS.seek 25.00ml @13:48:34.87 Vacuum too high -- waiting up to 40s @13:48:35.13 Intake @37.4psia, vacuum from 20.0 to 24.0psi @13:48:35.14 SS.reconfigure forSampling @13:48:35.17 SS.seek 25.00ml @13:48:36.15 Vacuum too high -- waiting up to 40s @13:48:36.41 Intake @37.1psia, vacuum from 20.0 to 24.0psi @13:48:36.42 SS.reconfigure forSampling @13:48:36.45 SS.seek 25.00ml @13:48:37.49 Vacuum too high -- waiting up to 40s @13:48:37.75 Intake @37.0psia, vacuum from 20.0 to 24.0psi @13:48:37.76 SS.reconfigure forSampling @13:48:37.79 SS.seek 25.00ml @13:48:38.81 Vacuum too high -- waiting up to 40s @13:48:39.07 Intake @36.9psia, vacuum from 20.0 to 24.0psi @13:48:39.08 SS.reconfigure forSampling @13:48:39.11 SS.seek 25.00ml @13:48:40.15 Vacuum too high -- waiting up to 40s @13:48:40.41 Intake @36.7psia, vacuum from 20.0 to 24.0psi @13:48:40.42 SS.reconfigure forSampling @13:48:40.45 SS.seek 25.00ml @13:48:41.71 Vacuum too high -- waiting up to 40s @13:48:41.97 Intake @36.9psia, vacuum from 20.0 to 24.0psi @13:48:41.98 SS.reconfigure forSampling @13:48:42.01 SS.seek 25.00ml @13:48:43.09 Vacuum too high -- waiting up to 40s @13:48:43.35 Intake @37.2psia, vacuum from 20.0 to 24.0psi @13:48:43.36 SS.reconfigure forSampling @13:48:43.39 SS.seek 25.00ml @13:48:44.35 Vacuum too high -- waiting up to 40s @13:48:44.61 Intake @37.3psia, vacuum from 20.0 to 24.0psi @13:48:44.62 SS.reconfigure forSampling @13:48:44.65 SS.seek 25.00ml @13:48:46.71 Vacuum too high -- waiting up to 40s @13:48:46.97 Intake @37.7psia, vacuum from 20.0 to 24.0psi @13:48:46.98 SS.reconfigure forSampling @13:48:47.02 SS.seek 25.00ml @13:48:48.05 Vacuum too high -- waiting up to 40s @13:48:48.31 Intake @37.2psia, vacuum from 20.0 to 24.0psi @13:48:48.33 SS.reconfigure forSampling @13:48:48.36 SS.seek 25.00ml @13:48:49.24 Vacuum too high -- waiting up to 40s @13:48:49.50 Intake @37.1psia, vacuum from 20.0 to 24.0psi @13:48:49.51 SS.reconfigure forSampling @13:48:49.55 SS.seek 25.00ml @13:48:50.55 Vacuum too high -- waiting up to 40s @13:48:50.82 SS.seek 25.00ml @13:48:51.68 Vacuum too high -- waiting up to 40s @13:48:51.94 Intake @36.8psia, vacuum from 20.0 to 24.0psi @13:48:51.95 SS.reconfigure forSampling @13:48:51.99 SS.seek 25.00ml @13:48:53.05 Vacuum too high -- waiting up to 40s @13:48:53.31 Intake @37.0psia, vacuum from 20.0 to 24.0psi @13:48:53.32 SS.reconfigure forSampling @13:48:53.36 SS.seek 25.00ml @13:48:54.43 Vacuum too high -- waiting up to 40s @13:48:54.69 Intake @37.1psia, vacuum from 20.0 to 24.0psi @13:48:54.70 SS.reconfigure forSampling @13:48:54.74 SS.seek 25.00ml @13:48:55.74 Vacuum too high -- waiting up to 40s @13:48:56.00 Intake @37.4psia, vacuum from 20.0 to 24.0psi @13:48:56.01 SS.reconfigure forSampling @13:48:56.05 SS.seek 25.00ml @13:48:56.96 Vacuum too high -- waiting up to 40s @13:48:57.22 Intake @36.7psia, vacuum from 20.0 to 24.0psi @13:48:57.23 SS.reconfigure forSampling @13:48:57.27 SS.seek 25.00ml @13:48:58.30 Vacuum too high -- waiting up to 40s @13:48:58.57 SS.seek 25.00ml @13:48:59.55 Vacuum too high -- waiting up to 40s @13:48:59.81 Intake @37.1psia, vacuum from 20.0 to 24.0psi @13:48:59.82 SS.reconfigure forSampling @13:48:59.86 SS.seek 25.00ml @13:49:00.87 Vacuum too high -- waiting up to 40s @13:49:01.13 Intake @37.5psia, vacuum from 20.0 to 24.0psi @13:49:01.14 SS.reconfigure forSampling @13:49:01.17 SS.seek 25.00ml @13:49:02.12 Vacuum too high -- waiting up to 40s @13:49:02.38 Intake @37.4psia, vacuum from 20.0 to 24.0psi @13:49:02.39 SS.reconfigure forSampling @13:49:02.42 SS.seek 25.00ml @13:49:03.37 Vacuum too high -- waiting up to 40s @13:49:03.63 Intake @36.8psia, vacuum from 20.0 to 24.0psi @13:49:03.64 SS.reconfigure forSampling @13:49:03.68 SS.seek 25.00ml @13:49:04.74 Vacuum too high -- waiting up to 40s @13:49:05.00 Intake @36.8psia, vacuum from 20.0 to 24.0psi @13:49:05.01 SS.reconfigure forSampling @13:49:05.04 SS.seek 25.00ml @13:49:05.90 Vacuum too high -- waiting up to 40s @13:49:06.16 Intake @37.0psia, vacuum from 20.0 to 24.0psi @13:49:06.17 SS.reconfigure forSampling @13:49:06.20 SS.seek 25.00ml @13:49:07.18 Vacuum too high -- waiting up to 40s @13:49:07.44 Intake @36.7psia, vacuum from 20.0 to 24.0psi @13:49:07.45 SS.reconfigure forSampling @13:49:07.48 SS.seek 25.00ml @13:49:08.43 Vacuum too high -- waiting up to 40s @13:49:08.69 Intake @36.8psia, vacuum from 20.0 to 24.0psi @13:49:08.70 SS.reconfigure forSampling @13:49:08.73 SS.seek 25.00ml @13:49:09.65 Vacuum too high -- waiting up to 40s @13:49:09.91 Intake @37.1psia, vacuum from 20.0 to 24.0psi @13:49:09.92 SS.reconfigure forSampling @13:49:09.95 SS.seek 25.00ml @13:49:10.90 Vacuum too high -- waiting up to 40s @13:49:11.16 Intake @37.1psia, vacuum from 20.0 to 24.0psi @13:49:11.17 SS.reconfigure forSampling @13:49:11.21 SS.seek 25.00ml @13:49:12.15 Vacuum too high -- waiting up to 40s @13:49:12.42 SS.seek 25.00ml @13:49:13.24 Vacuum too high -- waiting up to 40s @13:49:13.50 Intake @37.0psia, vacuum from 20.0 to 24.0psi @13:49:13.51 SS.reconfigure forSampling @13:49:13.54 SS.seek 25.00ml @13:49:14.49 Vacuum too high -- waiting up to 40s @13:49:14.75 Intake @36.8psia, vacuum from 20.0 to 24.0psi @13:49:14.76 SS.reconfigure forSampling @13:49:14.79 SS.seek 25.00ml @13:49:15.93 Vacuum too high -- waiting up to 40s @13:49:16.19 Intake @37.4psia, vacuum from 20.0 to 24.0psi @13:49:16.20 SS.reconfigure forSampling @13:49:16.23 SS.seek 25.00ml @13:49:17.08 Vacuum too high -- waiting up to 40s @13:49:17.34 Intake @37.7psia, vacuum from 20.0 to 24.0psi @13:49:17.35 SS.reconfigure forSampling @13:49:17.38 SS.seek 25.00ml @13:49:19.18 Vacuum too high -- waiting up to 40s @13:49:19.44 Intake @37.1psia, vacuum from 20.0 to 24.0psi @13:49:19.45 SS.reconfigure forSampling @13:49:19.49 SS.seek 25.00ml @13:49:20.40 Vacuum too high -- waiting up to 40s @13:49:20.66 Intake @37.0psia, vacuum from 20.0 to 24.0psi @13:49:20.67 SS.reconfigure forSampling @13:49:20.71 SS.seek 25.00ml @13:49:21.68 Vacuum too high -- waiting up to 40s @13:49:21.94 Intake @36.8psia, vacuum from 20.0 to 24.0psi @13:49:21.95 SS.reconfigure forSampling @13:49:21.99 SS.seek 25.00ml @13:49:22.90 Vacuum too high -- waiting up to 40s @13:49:23.16 Intake @36.8psia, vacuum from 20.0 to 24.0psi @13:49:23.17 SS.reconfigure forSampling @13:49:23.21 SS.seek 25.00ml @13:49:24.12 Vacuum too high -- waiting up to 40s @13:49:24.38 Intake @36.7psia, vacuum from 20.0 to 24.0psi @13:49:24.40 SS.reconfigure forSampling @13:49:24.43 SS.seek 25.00ml @13:49:25.46 Vacuum too high -- waiting up to 40s @13:49:25.72 Intake @37.1psia, vacuum from 20.0 to 24.0psi @13:49:25.73 SS.reconfigure forSampling @13:49:25.77 SS.seek 25.00ml @13:49:26.62 Vacuum too high -- waiting up to 40s @13:49:26.88 Intake @36.8psia, vacuum from 20.0 to 24.0psi @13:49:26.89 SS.reconfigure forSampling @13:49:26.93 SS.seek 25.00ml @13:49:27.99 Vacuum too high -- waiting up to 40s @13:49:28.25 Intake @37.0psia, vacuum from 20.0 to 24.0psi @13:49:28.26 SS.reconfigure forSampling @13:49:28.30 SS.seek 25.00ml @13:49:29.21 Vacuum too high -- waiting up to 40s @13:49:29.47 Intake @37.3psia, vacuum from 20.0 to 24.0psi @13:49:29.48 SS.reconfigure forSampling @13:49:29.52 SS.seek 25.00ml @13:49:30.37 Vacuum too high -- waiting up to 40s @13:49:30.64 SS.seek 25.00ml @13:49:31.52 Vacuum too high -- waiting up to 40s @13:49:31.78 Intake @37.3psia, vacuum from 20.0 to 24.0psi @13:49:31.79 SS.reconfigure forSampling @13:49:31.83 SS.seek 25.00ml @13:49:32.71 Vacuum too high -- waiting up to 40s @13:49:32.98 SS.seek 25.00ml @13:49:33.96 Vacuum too high -- waiting up to 40s @13:49:34.22 Intake @37.1psia, vacuum from 20.0 to 24.0psi @13:49:34.23 SS.reconfigure forSampling @13:49:34.27 SS.seek 25.00ml @13:49:35.18 Vacuum too high -- waiting up to 40s @13:49:35.44 Intake @36.9psia, vacuum from 20.0 to 24.0psi @13:49:35.45 SS.reconfigure forSampling @13:49:35.49 SS.seek 25.00ml @13:49:36.43 Vacuum too high -- waiting up to 40s @13:49:36.69 Intake @36.8psia, vacuum from 20.0 to 24.0psi @13:49:36.70 SS.reconfigure forSampling @13:49:36.73 SS.seek 25.00ml @13:49:37.71 Vacuum too high -- waiting up to 40s @13:49:37.97 Intake @37.0psia, vacuum from 20.0 to 24.0psi @13:49:37.98 SS.reconfigure forSampling @13:49:38.02 SS.seek 25.00ml @13:49:38.96 Vacuum too high -- waiting up to 40s @13:49:39.22 Intake @37.0psia, vacuum from 20.0 to 24.0psi @13:49:39.23 SS.reconfigure forSampling @13:49:39.27 SS.seek 25.00ml @13:49:40.11 Vacuum too high -- waiting up to 40s @13:49:40.37 Intake @36.9psia, vacuum from 20.0 to 24.0psi @13:49:40.38 SS.reconfigure forSampling @13:49:40.41 SS.seek 25.00ml @13:49:41.33 Vacuum too high -- waiting up to 40s @13:49:41.59 Intake @37.1psia, vacuum from 20.0 to 24.0psi @13:49:41.60 SS.reconfigure forSampling @13:49:41.64 SS.seek 25.00ml @13:49:42.49 Vacuum too high -- waiting up to 40s @13:49:42.75 Intake @37.6psia, vacuum from 20.0 to 24.0psi @13:49:42.76 SS.reconfigure forSampling @13:49:42.80 SS.seek 25.00ml @13:49:43.68 Vacuum too high -- waiting up to 40s @13:49:43.94 Intake @37.6psia, vacuum from 20.0 to 24.0psi @13:49:43.95 SS.reconfigure forSampling @13:49:43.98 SS.seek 25.00ml @13:49:44.96 Vacuum too high -- waiting up to 40s @13:49:45.22 Intake @37.2psia, vacuum from 20.0 to 24.0psi @13:49:45.23 SS.reconfigure forSampling @13:49:45.26 SS.seek 25.00ml @13:49:46.18 Vacuum too high -- waiting up to 40s @13:49:46.44 Intake @36.8psia, vacuum from 20.0 to 24.0psi @13:49:46.45 SS.reconfigure forSampling @13:49:46.48 SS.seek 25.00ml @13:49:47.40 Vacuum too high -- waiting up to 40s @13:49:47.87 Intake @37.0psia, vacuum from 20.0 to 24.0psi @13:49:47.88 SS.reconfigure forSampling @13:49:47.91 SS.seek 25.00ml @13:49:48.99 Vacuum too high -- waiting up to 40s @13:49:49.25 Intake @36.8psia, vacuum from 20.0 to 24.0psi @13:49:49.73 SS.reconfigure forSampling @13:49:49.76 SS.seek 25.00ml @13:49:51.21 Vacuum too high -- waiting up to 40s @13:49:51.48 SS.seek 25.00ml @13:49:52.33 Vacuum too high -- waiting up to 40s @13:49:52.59 Intake @37.2psia, vacuum from 20.0 to 24.0psi @13:49:52.60 SS.reconfigure forSampling @13:49:52.64 SS.seek 25.00ml @13:49:53.46 Vacuum too high -- waiting up to 40s @13:49:53.72 Intake @37.5psia, vacuum from 20.0 to 24.0psi @13:49:53.73 SS.reconfigure forSampling @13:49:53.77 SS.seek 25.00ml @13:49:54.61 Vacuum too high -- waiting up to 40s @13:49:54.87 Intake @37.1psia, vacuum from 20.0 to 24.0psi @13:49:54.88 SS.reconfigure forSampling @13:49:54.92 SS.seek 25.00ml @13:49:55.83 Vacuum too high -- waiting up to 40s @13:49:56.09 Intake @37.0psia, vacuum from 20.0 to 24.0psi @13:49:56.10 SS.reconfigure forSampling @13:49:56.14 SS.seek 25.00ml @13:49:57.05 Vacuum too high -- waiting up to 40s @13:49:57.53 Intake @37.2psia, vacuum from 20.0 to 24.0psi @13:49:57.54 SS.reconfigure forSampling @13:49:57.58 SS.seek 25.00ml @13:49:58.71 Vacuum too high -- waiting up to 40s @13:49:58.97 Intake @36.9psia, vacuum from 20.0 to 24.0psi @13:49:58.98 SS.reconfigure forSampling @13:49:59.01 SS.seek 25.00ml @13:49:59.93 Vacuum too high -- waiting up to 40s @13:50:00.19 Intake @36.8psia, vacuum from 20.0 to 24.0psi @13:50:00.20 SS.reconfigure forSampling @13:50:00.24 SS.seek 25.00ml @13:50:01.08 Vacuum too high -- waiting up to 40s @13:50:01.34 Intake @36.8psia, vacuum from 20.0 to 24.0psi @13:50:01.35 SS.reconfigure forSampling @13:50:01.39 SS.seek 25.00ml @13:50:02.27 Vacuum too high -- waiting up to 40s @13:50:02.53 Intake @37.0psia, vacuum from 20.0 to 24.0psi @13:50:02.54 SS.reconfigure forSampling @13:50:02.57 SS.seek 25.00ml @13:50:03.58 Vacuum too high -- waiting up to 40s @13:50:04.06 Intake @38.0psia, vacuum from 20.0 to 24.0psi @13:50:04.07 SS.reconfigure forSampling @13:50:04.10 SS.seek 25.00ml @13:50:05.33 Vacuum too high -- waiting up to 40s @13:50:05.59 Intake @37.0psia, vacuum from 20.0 to 24.0psi @13:50:05.60 SS.reconfigure forSampling @13:50:05.63 SS.seek 25.00ml @13:50:06.58 Vacuum too high -- waiting up to 40s @13:50:06.85 SS.seek 25.00ml @13:50:07.64 Vacuum too high -- waiting up to 40s @13:50:08.12 Intake @37.0psia, vacuum from 20.0 to 24.0psi @13:50:08.13 SS.reconfigure forSampling @13:50:08.16 SS.seek 25.00ml @13:50:09.27 Vacuum too high -- waiting up to 40s @13:50:09.53 Intake @36.8psia, vacuum from 20.0 to 24.0psi @13:50:09.54 SS.reconfigure forSampling @13:50:09.57 SS.seek 25.00ml @13:50:10.43 Vacuum too high -- waiting up to 40s @13:50:10.69 Intake @36.5psia, vacuum from 20.0 to 24.0psi @13:50:10.70 SS.reconfigure forSampling @13:50:10.73 SS.seek 25.00ml @13:50:11.74 Vacuum too high -- waiting up to 40s @13:50:12.21 Intake @36.8psia, vacuum from 20.0 to 24.0psi @13:50:12.22 SS.reconfigure forSampling @13:50:12.25 SS.seek 25.00ml @13:50:13.30 Vacuum too high -- waiting up to 40s @13:50:13.56 Intake @37.2psia, vacuum from 20.0 to 24.0psi @13:50:13.57 SS.reconfigure forSampling @13:50:13.60 SS.seek 25.00ml @13:50:14.49 Vacuum too high -- waiting up to 40s @13:50:14.75 Intake @37.4psia, vacuum from 20.0 to 24.0psi @13:50:14.76 SS.reconfigure forSampling @13:50:14.79 SS.seek 25.00ml @13:50:15.71 Vacuum too high -- waiting up to 40s @13:50:15.97 Intake @36.8psia, vacuum from 20.0 to 24.0psi @13:50:15.98 SS.reconfigure forSampling @13:50:16.01 SS.seek 25.00ml @13:50:16.96 Vacuum too high -- waiting up to 40s @13:50:17.43 Intake @36.6psia, vacuum from 20.0 to 24.0psi @13:50:17.44 SS.reconfigure forSampling @13:50:17.48 SS.seek 25.00ml @13:50:18.58 Vacuum too high -- waiting up to 40s @13:50:19.05 Intake @37.1psia, vacuum from 20.0 to 24.0psi @13:50:19.06 SS.reconfigure forSampling @13:50:19.09 SS.seek 25.00ml @13:50:20.11 Vacuum too high -- waiting up to 40s @13:50:20.37 Intake @36.9psia, vacuum from 20.0 to 24.0psi @13:50:20.38 SS.reconfigure forSampling @13:50:20.41 SS.seek 25.00ml @13:50:21.33 Vacuum too high -- waiting up to 40s @13:50:22.11 Intake @37.0psia, vacuum from 20.0 to 24.0psi @13:50:22.12 SS.reconfigure forSampling @13:50:22.16 SS.seek 25.00ml @13:50:23.61 Vacuum too high -- waiting up to 40s @13:50:23.87 Intake @36.7psia, vacuum from 20.0 to 24.0psi @13:50:23.89 SS.reconfigure forSampling @13:50:23.92 SS.seek 25.00ml @13:50:24.89 Vacuum too high -- waiting up to 40s @13:50:25.15 Intake @37.6psia, vacuum from 20.0 to 24.0psi @13:50:25.16 SS.reconfigure forSampling @13:50:25.20 SS.seek 25.00ml @13:50:26.05 Vacuum too high -- waiting up to 40s @13:50:26.31 Intake @37.2psia, vacuum from 20.0 to 24.0psi @13:50:26.32 SS.reconfigure forSampling @13:50:26.36 SS.seek 25.00ml @13:50:27.24 Vacuum too high -- waiting up to 40s @13:50:27.50 Intake @36.8psia, vacuum from 20.0 to 24.0psi @13:50:27.51 SS.reconfigure forSampling @13:50:27.55 SS.seek 25.00ml @13:50:28.46 Vacuum too high -- waiting up to 40s @13:50:28.73 SS.seek 25.00ml @13:50:29.64 Vacuum too high -- waiting up to 40s @13:50:29.90 Intake @36.8psia, vacuum from 20.0 to 24.0psi @13:50:29.91 SS.reconfigure forSampling @13:50:29.95 SS.seek 25.00ml @13:50:30.86 Vacuum too high -- waiting up to 40s @13:50:31.34 Intake @37.0psia, vacuum from 20.0 to 24.0psi @13:50:31.35 SS.reconfigure forSampling @13:50:31.39 SS.seek 25.00ml @13:50:32.49 Vacuum too high -- waiting up to 40s @13:50:32.75 Intake @37.1psia, vacuum from 20.0 to 24.0psi @13:50:32.76 SS.reconfigure forSampling @13:50:32.80 SS.seek 25.00ml @13:50:33.67 Vacuum too high -- waiting up to 40s @13:50:33.93 Intake @37.3psia, vacuum from 20.0 to 24.0psi @13:50:33.94 SS.reconfigure forSampling @13:50:33.98 SS.seek 25.00ml @13:50:34.77 Vacuum too high -- waiting up to 40s @13:50:35.05 SS.seek 25.00ml @13:50:35.92 Vacuum too high -- waiting up to 40s @13:50:36.18 Intake @37.2psia, vacuum from 20.0 to 24.0psi @13:50:36.19 SS.reconfigure forSampling @13:50:36.22 SS.seek 25.00ml @13:50:37.08 Vacuum too high -- waiting up to 40s @13:50:37.34 Intake @37.2psia, vacuum from 20.0 to 24.0psi @13:50:37.35 SS.reconfigure forSampling @13:50:37.38 SS.seek 25.00ml @13:50:38.24 Vacuum too high -- waiting up to 40s @13:50:38.50 Intake @37.1psia, vacuum from 20.0 to 24.0psi @13:50:38.51 SS.reconfigure forSampling @13:50:38.54 SS.seek 25.00ml @13:50:39.46 Vacuum too high -- waiting up to 40s @13:50:39.93 Intake @37.2psia, vacuum from 20.0 to 24.0psi @13:50:39.94 SS.reconfigure forSampling @13:50:39.97 SS.seek 25.00ml @13:50:41.08 Vacuum too high -- waiting up to 40s @13:50:41.55 Intake @37.5psia, vacuum from 20.0 to 24.0psi @13:50:41.56 SS.reconfigure forSampling @13:50:41.59 SS.seek 25.00ml @13:50:42.74 Vacuum too high -- waiting up to 40s @13:50:43.21 Intake @37.4psia, vacuum from 20.0 to 24.0psi @13:50:43.22 SS.reconfigure forSampling @13:50:43.25 SS.seek 25.00ml @13:50:44.33 Vacuum too high -- waiting up to 40s @13:50:44.59 Intake @36.8psia, vacuum from 20.0 to 24.0psi @13:50:44.60 SS.reconfigure forSampling @13:50:44.63 SS.seek 25.00ml @13:50:45.55 Vacuum too high -- waiting up to 40s @13:50:46.02 Intake @36.8psia, vacuum from 20.0 to 24.0psi @13:50:46.03 SS.reconfigure forSampling @13:50:46.06 SS.seek 25.00ml @13:50:47.14 Vacuum too high -- waiting up to 40s @13:50:47.40 Intake @36.9psia, vacuum from 20.0 to 24.0psi @13:50:47.41 SS.reconfigure forSampling @13:50:47.44 SS.seek 25.00ml @13:50:48.27 Vacuum too high -- waiting up to 40s @13:50:48.53 Intake @36.7psia, vacuum from 20.0 to 24.0psi @13:50:48.54 SS.reconfigure forSampling @13:50:48.57 SS.seek 25.00ml @13:50:49.55 Vacuum too high -- waiting up to 40s @13:50:50.02 Intake @37.0psia, vacuum from 20.0 to 24.0psi @13:50:50.03 SS.reconfigure forSampling @13:50:50.06 SS.seek 25.00ml @13:50:51.11 Vacuum too high -- waiting up to 40s @13:50:51.59 Intake @37.6psia, vacuum from 20.0 to 24.0psi @13:50:51.60 SS.reconfigure forSampling @13:50:51.63 SS.seek 25.00ml @13:50:52.77 Vacuum too high -- waiting up to 40s @13:50:53.78 Intake @37.2psia, vacuum from 20.0 to 24.0psi @13:50:53.81 SS.reconfigure forSampling @13:50:53.84 SS.seek 25.00ml @13:50:56.01 Stroke completed -- waiting for pressure to equalize ... @13:50:56.38 SS.reconfigure SSconfig @13:50:56.42 SS.hold @13:50:56.50 Intake.select :close @13:50:56.66 ShallowSampler::Clogged in MAIN -- progress too slow after 700.0ml SSV.dial :exhaust @13:50:57.43 Exhaust.select :open @13:50:57.54 SS.reconfigure SSexhale @13:50:57.58 SS.seek 0.00ml @13:51:06.18 Exhaust.select :close @13:51:06.28 Sampled 700.0ml @13:51:06.29 collectSample: Complete Evacuating Puck (bubblePt=55.0psi, delay=15.0s) @13:51:06.30 Intake.select :close @13:51:06.35 CSV.dial :waste @13:51:06.38 CS.seek 0.000ml @13:51:06.46 SSV.dial CBV,avoiding => exhaust @13:51:06.48
CBV.dial SSV @13:51:06.52 CSV.dial :air @13:51:07.34 CS.seek 10.000ml @13:51:08.40
CTV.dial :air @13:51:09.15 delay 15 seconds @13:51:09.27 Sleepy.power :sampler,:OFF @13:51:24.15
SSV.dial :spit,avoiding => exhaust @13:51:25.46 CTV.dial CSV,avoiding => intake @13:51:25.48
SS.seek 0.00ml @13:51:25.57 SSV.dial CBV,avoiding => exhaust @13:51:31.63 CBV.dial between CSV and waste2b @13:51:32.57 CTV.dial CSV,avoiding => air @13:51:32.59
CSV.dial CTV @13:51:33.25 CS.reconfigure CSpressurize @13:51:33.28 CS.seek 0.000ml @13:51:57.66 BubblePoint: Intake @70.3psia, Outlet @16.1psia @13:51:57.67 SS.reconfigure SSconfig @13:51:57.70 SS.seek 10.00ml @13:52:03.60 delay 15 seconds @13:52:18.70 Evacuated: Intake @69.3psia, Outlet @0.2psia SSV.dial :spit,avoiding => exhaust @13:52:20.02 SS.reconfigure SSexhale @13:52:20.05 SS.seek 0.00ml @13:52:25.33 SS.reconfigure SSfast @13:52:25.37 SS.seek 5.00ml @13:52:28.22 SSV.dial CBV,avoiding => exhaust @13:52:28.24
CBV.dial SSV @13:52:29.56 CTV.dial :air,avoiding => intake @13:52:30.85 CSV.dial CTV @13:52:30.86
SS.reconfigure SSconfig @13:52:30.90 SS.seek 10.00ml @13:52:31.86 CSV.dial :air @13:52:32.29 CS.reconfigure CSfast @13:52:32.32 CS.seek 0.000ml @13:52:33.87
SSV.dial :spit,avoiding => exhaust @13:52:34.48 CS.coast @13:52:35.17
SS.reconfigure SSexhale @13:52:35.20 SS.seek 0.00ml @13:52:40.22 SS.reconfigure SSfast @13:52:40.25 SS.seek 5.00ml @13:52:43.23 SSV.dial CBV,avoiding => exhaust @13:52:43.25
CBV.dial SSV @13:52:45.54 SSV.dial :spit,avoiding => exhaust @13:52:46.84 SS.reconfigure SSconfig @13:52:46.87 SS.seek 0.00ml @13:52:50.10 SS.coast @13:52:50.13 Evacuating Puck (bubblePt=55.0psi, delay=15.0s) Intake.select :close @13:52:50.19 CSV.dial :waste @13:52:50.28
SSV.dial :exhaust @13:52:50.93 CS.seek 0.000ml @13:52:51.01
Exhaust.select :open @13:52:51.11 SS.reconfigure SSexhale @13:52:51.15 SS.seek 0.00ml @13:52:51.19 CSV.dial :air @13:52:51.61
Exhaust.select :close @13:52:51.70 SSV.dial CBV,avoiding => exhaust @13:52:51.72
CBV.dial SSV @13:52:52.01 CS.seek 10.000ml @13:52:53.58
CTV.dial :air @13:52:53.59 delay 15 seconds @13:53:08.59 SSV.dial :spit,avoiding => exhaust @13:53:09.92 CTV.dial CSV,avoiding => intake @13:53:09.94
SS.seek 0.00ml @13:53:10.03 SSV.dial CBV,avoiding => exhaust @13:53:15.59 CBV.dial between CSV and waste2b @13:53:16.53 CSV.dial between air and CTV @13:53:16.82 CS.seek 7.062ml @13:53:24.37 CTV.dial CSV,avoiding => air @13:53:24.38
CSV.dial CTV @13:53:24.69 CS.reconfigure CSpressurize @13:53:24.72 CS.seek 0.000ml @13:53:43.14 CSV.dial :air @13:53:43.66 CS.reconfigure CSfast @13:53:43.69 CS.seek 10.000ml @13:54:06.99 CSV.dial between air and CTV @13:54:07.38 CS.seek 2.243ml @13:54:26.23 CTV.dial CSV,avoiding => air @13:54:26.25
CSV.dial CTV @13:54:26.55 CS.reconfigure CSpressurize @13:54:26.58 CS.seek 0.000ml @13:54:28.81 BubblePoint: Intake @74.0psia, Outlet @18.6psia @13:54:28.82 SS.reconfigure SSconfig @13:54:28.85 SS.seek 10.00ml @13:54:34.73 delay 15 seconds @13:54:49.83 Evacuated: Intake @73.2psia, Outlet @0.2psia @13:54:49.84 SSV.dial :spit,avoiding => exhaust @13:54:51.17 SS.reconfigure SSexhale @13:54:51.20 SS.seek 0.00ml @13:54:56.09 SS.reconfigure SSfast @13:54:56.12 SS.seek 5.00ml @13:54:59.01 SSV.dial CBV,avoiding => exhaust @13:54:59.03
CBV.dial SSV @13:55:00.35 CTV.dial :air,avoiding => intake @13:55:01.65 CSV.dial CTV @13:55:01.67
SS.reconfigure SSconfig @13:55:01.71 SS.seek 10.00ml @13:55:02.67 CSV.dial :air @13:55:03.19 CS.reconfigure CSfast @13:55:03.22 CS.seek 0.000ml @13:55:04.97
SSV.dial :spit,avoiding => exhaust @13:55:06.30 SS.reconfigure SSexhale @13:55:06.33 SS.seek 0.00ml @13:55:07.57 CS.coast @13:55:11.59
SS.reconfigure SSfast @13:55:11.63 SS.seek 5.00ml @13:55:14.60 SSV.dial CBV,avoiding => exhaust @13:55:14.62
CBV.dial SSV @13:55:16.93 SSV.dial :spit,avoiding => exhaust @13:55:18.26 SS.reconfigure SSconfig @13:55:18.29 SS.seek 0.00ml @13:55:21.59 SS.coast @13:55:21.62 Lysing: Adding 1.5ml lysis CBV.dial between CSV and waste1,avoiding => DBV @13:55:23.11 CTV.dial CSV @13:55:24.35 CSV.dial CSR @13:55:25.13 CSR.select :lysis @13:55:25.23 CS.reconfigure CSconfig @13:55:25.27 CS.seek 1.000ml @13:55:28.63 delay 5 seconds @13:55:33.65 CSR.select :air @13:55:33.79 CS.seek 6.000ml @13:55:47.29 delay 2 seconds @13:55:49.30 CSV.dial :waste1 @13:55:50.56 CS.reconfigure CSfast @13:55:50.59 CS.seek empty @13:56:04.88 CSV.dial CSR @13:56:06.55 CSR.select :lysis @13:56:06.65 CS.reconfigure CSconfig @13:56:06.69 CS.seek 1.500ml @13:56:11.29 delay 5 seconds @13:56:16.30 CSR.select :air @13:56:16.44 CS.seek 4.500ml @13:56:24.78 delay 2 seconds @13:56:26.80 CSV.dial CTV @13:56:27.31 CS.seek 1.275ml @13:56:36.30 Heating sh1 puck from tube 2 in Collection Clamp to 85.0C for 5:00 @13:56:36.31 CH.seek 85.0C,8:00 @13:59:26.41 CH.hold 5:00,85.0C @14:04:26.51 Collection Puck Heater at 85.0C after lysing @14:04:26.52 CS.seek 0.000ml @14:04:30.63 CTV.dial between spare and air,avoiding => intake @14:04:31.62 CSV.dial :air @14:04:32.06 CS.seek 5.000ml @14:04:45.55 CSV.dial CTV @14:04:46.05 delay 2 seconds @14:04:48.06 CSV.dial :air @14:04:48.55 delay 2 seconds @14:04:50.56 CSV.dial CTV @14:04:51.05 delay 2 seconds @14:04:53.06 CSV.dial :air @14:04:53.55 delay 2 seconds @14:04:55.56 CS.seek 0.000ml @14:05:09.08 Recovering Lysate into Collection syringe @14:05:09.09 CSV.dial CBV @14:05:10.08 CBV.dial CSV @14:05:10.43 CS.seek 5.000ml @14:05:23.93 delay 5 seconds @14:05:28.94 CTV.dial :air @14:05:29.43 CS.seek 7.001ml @14:05:35.33 delay 5 seconds @14:05:40.34 CSV.dial :air2 @14:05:40.76 CS.seek 10.000ml @14:05:49.18 CSV.dial between air and CTV,avoiding => CBV @14:05:50.31 Lysing Complete @14:05:50.36 Storing sh1 puck in Collection Clamp to tube 1 @14:05:50.48 SC.reconfigure SCtight @14:05:50.52 SC.seek 1 @14:05:52.86 SC.reconfigure SCloose @14:05:53.02 Elbow.reconfigure TightElbow @14:05:53.06 Elbow.seek 1 @14:05:54.65 Forearm.reconfigure TightForearm @14:05:54.68 Forearm.seek 1 + 800 counts @14:05:55.69 SE.reconfigure SEpick @14:05:55.74 SE.seek beamBreak @14:05:58.48 SE.configure SEconfig @14:05:58.60 Elbow.reconfigure LimpElbow @14:05:58.63 Forearm.reconfigure LooseForearm @14:05:58.67 Forearm.seek 1 @14:05:58.89 Elbow.hold @14:05:58.94 Elbow.reconfigure TightElbow @14:05:59.02 SE.seek 2.04 Pucks below Top Plate @14:05:59.32 Forearm.coast @14:05:59.35 Forearm.seek 1 + 308 counts @14:05:59.58 Forearm.reconfigure TightForearm @14:05:59.61 Forearm.seek 1 + 800 counts @14:06:00.47 SE.seek 3.04 Pucks below Top Plate @14:06:01.03 Forearm.reconfigure LooseForearm @14:06:01.06 Forearm.seek retract @14:06:02.03 Elbow.seek CC @14:06:03.60 Elbow.reconfigure LooseElbow @14:06:03.68 Forearm.seek CC @14:06:04.67 CC.seek sealed @14:06:05.61 Hand.grip @14:06:06.29 CC.seek open @14:06:09.63 Forearm.seek retract @14:06:10.56 Elbow.reconfigure TightElbow @14:06:10.60 Elbow.seek 1 @14:06:12.25 Elbow.reconfigure LimpElbow @14:06:12.30 Forearm.seek 1 @14:06:13.30 Elbow.hold @14:06:13.36 Elbow.reconfigure TightElbow @14:06:13.40 Hand.release @14:06:14.25 Forearm.coast @14:06:14.29 Forearm.seek 1 + 318 counts @14:06:14.52 Forearm.reconfigure TightForearm @14:06:14.55 Forearm.seek 1 + 800 counts @14:06:14.83 SE.seek down @14:06:15.94
Forearm.reconfigure LooseForearm @14:06:15.97 Forearm.seek retract @14:06:17.25 SE.coast @14:06:17.28
SC.coast @14:06:17.30 Tranferring lysate to PRV @14:06:17.31 CSV.dial :air @14:06:17.60 CS.seek 10.000ml @14:06:17.64 CSV.dial between air and CTV @14:06:18.21 AIV.dial :bypass @14:06:18.37 Beginning samplePassC @14:06:18.38 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:06:18.52 Elbow.seek FlushPuck::Garage @14:06:20.25 Elbow.reconfigure LooseElbow @14:06:20.33 Forearm.seek garage @14:06:21.34 Hand.grip @14:06:22.13 Forearm.seek retract @14:06:23.14 Elbow.reconfigure TightElbow @14:06:23.18 Elbow.seek CC @14:06:25.99 Elbow.reconfigure LooseElbow @14:06:26.08 Forearm.reconfigure TightForearm @14:06:26.11 Forearm.seek CC @14:06:27.19 CC.seek closed @14:06:30.98 Collection Clamp onSpring/offSpring for 5/22 samples, on/off torque 2.04 @14:06:31.03 Hand.release @14:06:31.83 Forearm.reconfigure LooseForearm @14:06:31.86 Forearm.seek retract @14:06:32.81 AWV.select :air @14:06:32.82 AIV.dial :bypass @14:06:32.83 PSV.dial PSR @14:06:34.11 PSR.select :flush @14:06:34.21 PS.seek 1.500ml @14:06:50.25 delay 5 seconds @14:06:55.27 PSV.dial :waste1 @14:06:55.71 PS.seek 0.000ml @14:07:11.78 PSV.dial PSR @14:07:12.24 PSR.select :air @14:07:12.34 PS.seek 2.500ml @14:07:38.60 CBV.dial PRV @14:07:38.61 PRV.dial CBV @14:07:39.39
PSV.dial :mixloop @14:07:40.50 CTV.dial :air,avoiding => intake @14:07:40.51 PTV.dial :PRVmixing @14:07:40.59 PS.seek 0.000ml @14:08:07.03 PSV.dial :air @14:08:07.49 PS.seek 2.500ml @14:08:33.75 PSV.dial :mixloop @14:08:34.20 PS.seek 0.000ml @14:09:00.47 PSV.dial :air @14:09:01.62 PS.seek 2.500ml @14:09:27.93 PSV.dial :mixloop @14:09:28.36 PS.seek 0.000ml @14:09:54.56 Garaging FlushPuck in Collection Clamp @14:09:54.62 Elbow.reconfigure TightElbow @14:09:54.65 Elbow.seek CC @14:09:54.81 Elbow.reconfigure LooseElbow @14:09:54.90 Forearm.seek CC @14:09:55.87 CC.seek sealed @14:09:56.82 Hand.grip @14:09:57.53 CC.seek open @14:10:00.81 Forearm.seek retract @14:10:01.80 Elbow.reconfigure TightElbow @14:10:01.84 Elbow.seek garage @14:10:04.62 Elbow.reconfigure LooseElbow @14:10:04.70 Forearm.reconfigure TightForearm @14:10:04.73 Forearm.seek garage @14:10:05.94 Hand.release @14:10:06.69 Forearm.reconfigure LooseForearm @14:10:06.72 Forearm.seek retract @14:10:07.86 Connecting PS to CS to transfer sample @14:10:07.87 CBV.dial AIV @14:10:07.88 AIV.dial :bypass @14:10:07.89 PSV.dial :mixloop @14:10:07.90 PTV.dial :PRVmixing @14:10:07.91 PRV.dial AIV CTV.dial :air,avoiding => intake @14:10:07.92 Loading lyfil puck from tube 2 into Collection Clamp @14:10:08.09 SC.reconfigure SCtight @14:10:08.13 SC.seek 2 @14:10:10.41 SC.reconfigure SCloose @14:10:10.62 Elbow.reconfigure TightElbow @14:10:10.68 Elbow.seek 2 @14:10:12.53 Forearm.reconfigure TightForearm @14:10:12.56 Forearm.seek 2 + 800 counts @14:10:13.63 SE.reconfigure SEpick @14:10:13.68 SE.seek beamBreak @14:10:14.95 SE.configure SEconfig @14:10:15.07 Elbow.seek 2 @14:10:15.36 Forearm.seek 2 + 300 counts @14:10:15.58 Forearm.reconfigure LooseForearm @14:10:15.62 Hand.grip @14:10:16.41 SE.seek down @14:10:16.55
Forearm.seek retract @14:10:17.48 SE.coast @14:10:17.55
Elbow.seek CC @14:10:19.12 Elbow.reconfigure LooseElbow @14:10:19.20 Forearm.reconfigure TightForearm @14:10:19.24 Forearm.seek CC @14:10:20.35 CC.seek closed @14:10:24.12 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 2.05 @14:10:24.13 Hand.release @14:10:24.94 Forearm.reconfigure LooseForearm @14:10:24.97 Forearm.seek retract @14:10:25.91 SC.coast @14:10:25.94 CTV.dial CSV,avoiding => intake @14:10:27.24 CSV.dial CTV @14:10:27.61 CS.seek 5.000ml @14:10:41.16 delay 1:20 @14:12:01.17 CS.seek 2.500ml @14:12:08.38 delay 40 seconds @14:12:48.39 CS.seek 0.000ml @14:12:55.47 delay 1:00 @14:13:55.49 PSV.dial :air @14:13:55.94 PS.seek 2.500ml @14:14:22.21 PSV.dial :mixloop @14:14:22.65 delay 3 seconds @14:14:25.66 PSV.dial :air @14:14:26.10 delay 2 seconds @14:14:28.11 PSV.dial :mixloop @14:14:28.54 delay 3 seconds @14:14:31.55 PSV.dial :waste1 @14:14:32.85 PS.seek empty @14:14:59.05 CBV.dial :waste1 @14:14:59.52 delay 5 seconds @14:15:04.53 CBV.dial AIV @14:15:04.99 delay 10 seconds @14:15:15.00 CBV.dial :waste1 @14:15:15.47 delay 10 seconds @14:15:25.48 CBV.dial AIV @14:15:25.95 delay 10 seconds @14:15:35.96 CTV.dial CSV,avoiding => intake @14:15:35.97 CBV.dial between AIV and waste1 @14:15:36.30 CSV.dial :air @14:15:37.35 CS.seek 10.000ml @14:16:03.44 CSV.dial CTV @14:16:03.95 CS.seek 0.000ml @14:16:30.00 CBV.dial :waste1 @14:16:30.29 delay 10 seconds @14:16:40.30 CTV.dial :air,avoiding => intake @14:16:41.64 Storing lyfil puck in Collection Clamp to tube 1 @14:16:41.77 SC.reconfigure SCtight @14:16:41.80 SC.seek 1 @14:16:44.03 SC.reconfigure SCloose @14:16:44.21 Elbow.reconfigure TightElbow @14:16:44.25 Elbow.seek 1 @14:16:45.84 Forearm.reconfigure TightForearm @14:16:45.88 Forearm.seek 1 + 800 counts @14:16:47.06 SE.reconfigure SEpick @14:16:47.11 SE.seek beamBreak @14:16:49.85 SE.configure SEconfig @14:16:49.97 Elbow.reconfigure LimpElbow @14:16:50.00 Forearm.reconfigure LooseForearm @14:16:50.04 Forearm.seek 1 @14:16:50.27 Elbow.hold @14:16:50.31 Elbow.reconfigure TightElbow @14:16:50.38 SE.seek 3.04 Pucks below Top Plate @14:16:50.70 Forearm.coast @14:16:50.73 Forearm.seek 1 + 336 counts @14:16:51.02 Forearm.reconfigure TightForearm @14:16:51.05 Forearm.seek 1 + 800 counts @14:16:51.33 SE.seek 4.04 Pucks below Top Plate @14:16:52.05 Forearm.reconfigure LooseForearm @14:16:52.08 Forearm.seek retract @14:16:53.02 Elbow.seek CC @14:16:54.55 Elbow.reconfigure LooseElbow @14:16:54.64 Forearm.seek CC @14:16:55.60 CC.seek sealed @14:16:56.50 Hand.grip @14:16:57.27 CC.seek open @14:17:00.60 Forearm.seek retract @14:17:01.54 Elbow.reconfigure TightElbow @14:17:01.58 Elbow.seek 1 @14:17:03.16 Elbow.reconfigure LimpElbow @14:17:03.20 Forearm.seek 1 @14:17:04.21 Elbow.hold @14:17:04.26 Elbow.reconfigure TightElbow @14:17:04.30 Hand.release @14:17:05.10 Forearm.coast @14:17:05.13 Forearm.seek 1 + 321 counts @14:17:05.36 Forearm.reconfigure TightForearm @14:17:05.39 Forearm.seek 1 + 800 counts @14:17:05.70 SE.seek down @14:17:06.80
Forearm.reconfigure LooseForearm @14:17:06.84 Forearm.seek retract @14:17:08.14 SE.coast @14:17:08.17
SC.coast @14:17:08.19 PSV.dial :air @14:17:09.25 PS.seek 0.499ml @14:17:15.11 delay 3 seconds @14:17:18.12 PSV.dial :mixloop @14:17:18.55 PRV.dial :air @14:17:18.99 delay 3 seconds @14:17:22.00 PRV.dial CBV @14:17:22.44 CBV.dial AIV @14:17:22.90 PRV.dial between CBV and air @14:17:23.21 PRV.dial :air @14:17:23.56 PS.reconfigure PSslow @14:17:23.60 PS.seek 0.599ml @14:17:26.14 PRV.dial :waste2 @14:17:26.87 PS.reconfigure PSconfig @14:17:26.90 PS.seek empty @14:17:33.80 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:17:33.95 Elbow.seek FlushPuck::Garage @14:17:35.72 Elbow.reconfigure LooseElbow @14:17:35.81 Forearm.seek garage @14:17:36.85 Hand.grip @14:17:37.72 Forearm.seek retract @14:17:38.73 Elbow.reconfigure TightElbow @14:17:38.77 Elbow.seek CC @14:17:42.13 Elbow.reconfigure LooseElbow @14:17:42.22 Forearm.reconfigure TightForearm @14:17:42.25 Forearm.seek CC @14:17:43.44 CC.seek closed @14:17:47.20 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 2.08 @14:17:47.25 Hand.release @14:17:48.06 Forearm.reconfigure LooseForearm @14:17:48.09 Forearm.seek retract @14:17:49.11 Clearing PML for modified lysate CTV.dial :air,avoiding => intake @14:17:49.12 PRV.dial :waste2 @14:17:49.13 PTV.dial :PRV,avoiding => puck @14:17:49.85 delay 2 seconds @14:17:51.85 PTV.dial :PRVmixing,avoiding => puck @14:17:52.56 delay 2 seconds @14:17:54.57 PSV.dial :PML @14:17:54.58 delay 2 seconds @14:17:56.58 PRV.dial :air @14:17:57.31 delay 2 seconds @14:17:59.37 PS.seek 1.499ml @14:18:15.48 delay 5 seconds @14:18:20.50 PS.seek 2.500ml @14:18:31.51 PSV.dial :air @14:18:31.98 PS.seek 0.000ml @14:18:58.42 mixing lysate with speDil @14:18:58.43 PSV.dial :PML @14:18:58.87 CBV.dial :PRV @14:18:58.88 PRV.dial :air @14:18:58.89 delay 2 seconds @14:19:00.90 PRV.dial :speDil @14:19:01.34 delay 2 seconds @14:19:03.40 PS.reconfigure PSslow1 @14:19:03.43 PS.seek 0.225ml @14:19:26.61 delay 5 seconds @14:19:31.62 PRV.dial CBV @14:19:32.34 delay 2 seconds @14:19:34.40 PS.seek 0.475ml @14:20:00.29 delay 5 seconds @14:20:05.30 PRV.dial :air @14:20:05.73 delay 2 seconds @14:20:07.74 PS.seek 1.000ml @14:21:01.41 delay 10 seconds @14:21:11.42 PRV.dial CBV @14:21:11.87 CTV.dial :air,avoiding => intake @14:21:11.88 PSV.dial :air @14:21:12.33 PS.reconfigure PSconfig @14:21:12.36 PS.seek 2.500ml @14:21:28.41 delay 3 seconds @14:21:31.43 PSV.dial PTV @14:21:32.15 PTV.dial :PRV,avoiding => puck @14:21:32.88 PS.seek 0.000ml @14:21:59.16 PSV.dial :air @14:21:59.91 PS.seek 2.500ml @14:22:26.44 delay 3 seconds @14:22:29.45 PSV.dial PTV @14:22:30.29 PTV.dial :PRV,avoiding => puck @14:22:30.31 PS.seek 0.000ml @14:22:56.53 Pushing modified lysate into AIV Injection Loop @14:22:56.54 CBV.dial AIV @14:22:56.55 AIV.dial :bypass @14:22:56.56 PRV.dial CBV @14:22:56.57 PSV.dial :air @14:22:57.28 PS.seek 2.500ml @14:23:23.57 delay 3 seconds @14:23:26.59 PSV.dial :PML @14:23:27.03 PTV.dial :PRVmixing,avoiding => puck @14:23:27.80 PS.reconfigure PSslow1 @14:23:27.83 PS.seek 1.740ml @14:24:45.47 delay 5 seconds @14:24:50.48 AIV.dial :sample @14:24:51.01 AWV.select :waste @14:24:51.16 PS.reconfigure PSslow2 @14:24:51.19 PS.seek 1.220ml @14:26:37.09 delay 15 seconds @14:26:52.10 AIV.dial :bypass @14:26:52.64 PS.reconfigure PSconfig @14:26:52.67 PS.seek 0.000ml @14:27:05.87 PSV.dial :air @14:27:06.44 PS.seek 2.500ml @14:27:32.68 PSV.dial :PML @14:27:33.11 PS.seek 0.000ml @14:27:59.34 AIV.dial :coilToMFB @14:27:59.35 AWV.select :air @14:27:59.49 Extracting Nucleic Acid from AIV @14:27:59.51
Beginning samplePassD @14:27:59.52 SPE.hold 2:46:40,55.0C @14:27:59.54 ASV.dial :SPEdelivery @14:27:59.66
AWV.select :air @14:27:59.67 AIV.dial :bypass @14:27:59.68 PSV.dial PSR @14:28:00.09 delay 20 seconds @14:28:00.71
PSR.select :killMFB @14:28:00.81 PS.seek 1.500ml @14:28:16.83 delay 5 seconds @14:28:20.10 primeLysateINT @14:28:20.11 AWV.select :air @14:28:20.12 AIV.dial :coilToMFB @14:28:20.67 ATV.dial :AIV @14:28:21.84
PSV.dial :waste1 @14:28:22.00 AS.reconfigure ASslow1 @14:28:22.03 AS.seek 0.241ml @14:28:22.28
PS.seek 0.000ml @14:28:38.33 PSV.dial PSR @14:28:38.79 PSR.select :air @14:28:38.89 PS.seek 2.500ml @14:29:05.18 PRV.dial CBV @14:29:05.20
CBV.dial PRV @14:29:05.21 PSV.dial :mixloop @14:29:06.24 CTV.dial :air,avoiding => intake @14:29:06.25 PTV.dial :PRVmixing @14:29:06.27 PS.seek 0.000ml @14:29:11.67 delay 10 seconds @14:29:21.68 ATV.dial :air2 @14:29:22.26 AS.seek 0.271ml @14:29:28.54 ATV.dial :waste2 @14:29:28.90 AS.reconfigure ASnorm @14:29:28.93 AS.seek empty @14:29:32.46
PSV.dial PSR @14:29:33.49 PSR.select :flush @14:29:33.59 PS.seek 1.500ml @14:29:43.18 Lysate primed! @14:29:43.19 AFV.select :holdingCoil @14:29:43.20 ATV.dial :air2 @14:29:43.59 AS.reconfigure ASslow1 @14:29:43.62 AS.seek 0.069ml @14:29:49.64
delay 5 seconds @14:29:54.65 PSV.dial :waste1 @14:29:55.09 PS.seek 0.000ml @14:29:58.18 delay 3 seconds @14:30:01.19 ATV.dial :AIV @14:30:01.76 AS.seek 0.469ml @14:30:11.17
PSV.dial PSR @14:30:11.63 PSR.select :air @14:30:11.73 PS.seek 2.500ml @14:30:38.06 PRV.dial CBV @14:30:38.08
CBV.dial PRV @14:30:38.09 PSV.dial :mixloop @14:30:39.10 CTV.dial :air,avoiding => intake @14:30:39.11 PTV.dial :PRVmixing @14:30:39.13 PS.seek 0.000ml @14:31:05.34 PSV.dial PSR @14:31:06.35 PSR.select :flush @14:31:06.45 PS.seek 1.500ml @14:31:22.49 delay 5 seconds @14:31:23.61 delay 5 seconds @14:31:27.50
PSV.dial :waste1 @14:31:27.94 PS.seek 0.000ml @14:31:28.66 Solid Phase Extraction Heater at 53.3C @14:31:28.67 ATV.dial ASV @14:31:29.00 ASV.dial :SPEdelivery @14:31:29.06 AS.seek 0.041ml @14:31:44.02
PSV.dial PSR @14:31:44.48 PSR.select :air @14:31:44.58 PS.seek 2.500ml @14:32:10.87 PRV.dial CBV @14:32:10.89
CBV.dial PRV @14:32:10.90 PSV.dial :mixloop @14:32:12.02 CTV.dial :air,avoiding => intake @14:32:12.03 PTV.dial :PRVmixing @14:32:12.05 PS.seek 0.000ml @14:32:38.30 PSV.dial PSR @14:32:39.30 PSR.select :flush @14:32:39.40 PS.seek 1.500ml @14:32:55.55 delay 5 seconds @14:32:56.61 delay 10 seconds @14:33:00.56
PSV.dial :waste1 @14:33:01.00 PS.seek 0.000ml @14:33:06.62 rinseLysateLineINT @14:33:06.63 AFV.select :carrierSolution @14:33:06.72 delay 2 seconds @14:33:08.73 AS.reconfigure ASconfig @14:33:08.76 AS.seek 1.000ml @14:33:17.11
PSV.dial PSR @14:33:17.55 PSR.select :air @14:33:17.65 PS.seek 2.500ml @14:33:43.83 PRV.dial CBV @14:33:43.85
CBV.dial PRV @14:33:43.86 PSV.dial :mixloop @14:33:44.86 CTV.dial :air,avoiding => intake @14:33:44.87 PTV.dial :PRVmixing @14:33:44.88 PS.reconfigure PSslow @14:33:44.92 PS.seek 1.200ml @14:34:04.95 delay 1 second @14:34:05.96 AFV.select :holdingCoil @14:34:06.05 delay 1 second @14:34:07.06 ATV.dial :AIV @14:34:07.40 AIV.dial :coilToMFB @14:34:07.41 AWV.select :waste @14:34:07.51 AS.reconfigure ASslow0 @14:34:07.55 AS.seek 0.000ml @14:34:11.89
PSV.dial :waste1 @14:34:13.20 PS.reconfigure PSconfig @14:34:13.23 PS.seek 0.000ml @14:34:26.27 PSV.dial :air @14:34:27.29 PS.seek 2.500ml @14:34:53.55 PSV.dial :mixloop @14:34:53.99 PS.reconfigure PSslow @14:34:54.02 PS.seek 0.000ml @14:35:45.33 PSV.dial :air @14:35:45.77 PS.reconfigure PSconfig @14:35:45.80 PS.seek 2.500ml @14:35:49.90 ATV.dial :air2 @14:35:50.46 AS.reconfigure ASslow1 @14:35:50.49 AS.seek 0.050ml @14:36:00.98 ATV.dial :AIV @14:36:01.65 AS.seek 0.010ml @14:36:10.09 ATV.dial :waste @14:36:11.83 AS.seek empty @14:36:11.98
PSV.dial :mixloop @14:36:12.43 PS.reconfigure PSslow @14:36:12.46 PS.seek 0.000ml @14:36:14.24 ethanol wash @14:36:14.25 ATV.dial :air2 @14:36:15.18 AS.reconfigure ASslow2 @14:36:15.21 AS.seek 0.009ml @14:36:25.77 delay 3 seconds @14:36:28.78 Pulling 0.4ml of Ethanol Wash @14:36:28.79 ATV.dial :ethanol @14:36:29.35 AS.reconfigure ASslow1 @14:36:29.38 AS.seek 0.409ml @14:37:03.73
Cleaning Collection Syringe @14:37:03.74 CSV.dial CSR @14:37:04.17 CSR.select :flush @14:37:04.27 CS.seek 4.000ml @14:37:15.26 delay 5 seconds @14:37:20.27 CSR.select :air @14:37:20.36 CS.seek 10.000ml @14:37:36.39 CSV.dial CTV @14:37:36.41
CTV.dial CSV @14:37:37.71 CBV.dial :waste1 @14:37:38.19 CS.seek 0.000ml @14:37:51.22 delay 3 seconds @14:37:54.23 ATV.dial :air1 @14:37:54.55 delay 3 seconds @14:37:57.61 AS.seek 0.434ml @14:38:02.97 delay 3 seconds @14:38:04.35
CSV.dial CSR @14:38:04.85 CSR.select :flush @14:38:04.95 CS.seek 4.000ml @14:38:05.98 ATV.dial :ethanol @14:38:06.37 AS.seek 0.419ml @14:38:09.67 delay 3 seconds @14:38:12.68 ATV.dial ASV @14:38:13.79 AS.seek 0.092ml @14:38:15.92
delay 5 seconds @14:38:20.93 CSR.select :air @14:38:21.02 CS.seek 10.000ml @14:38:37.10 CSV.dial CTV @14:38:37.12
CTV.dial CSV @14:38:37.63 CBV.dial :waste1 @14:38:37.65 CS.seek 0.000ml @14:39:03.81 Garaging FlushPuck in Collection Clamp @14:39:03.86 Elbow.reconfigure TightElbow @14:39:03.89 Elbow.seek CC @14:39:03.99 Elbow.reconfigure LooseElbow @14:39:04.07 Forearm.seek CC @14:39:05.05 CC.seek sealed @14:39:05.98 Hand.grip @14:39:06.72 CC.seek open @14:39:10.03 Forearm.seek retract @14:39:10.98 Elbow.reconfigure TightElbow @14:39:11.02 Elbow.seek garage @14:39:13.84 Elbow.reconfigure LooseElbow @14:39:13.92 Forearm.reconfigure TightForearm @14:39:13.95 Forearm.seek garage @14:39:15.13 Hand.release @14:39:15.88 Forearm.reconfigure LooseForearm @14:39:15.91 Forearm.seek retract @14:39:16.94 Whole Cell Archive sampling 1000ml @14:39:16.95 Loading wcr puck from tube 2 into Collection Clamp @14:39:17.13 SC.reconfigure SCtight @14:39:17.16 SC.seek 2 @14:39:19.42 SC.reconfigure SCloose @14:39:19.60 Elbow.reconfigure TightElbow @14:39:19.63 Elbow.seek 2 @14:39:20.74 delay 3 seconds @14:39:21.47 Forearm.reconfigure TightForearm @14:39:21.50 Forearm.seek 2 + 800 counts @14:39:22.48 SE.reconfigure SEpick @14:39:22.53 SE.seek beamBreak @14:39:23.75 ATV.dial :waste2 @14:39:23.88 SE.configure SEconfig @14:39:24.00 Elbow.seek 2 @14:39:24.14 Forearm.seek 2 + 300 counts @14:39:24.28 AS.reconfigure ASconfig @14:39:24.31 AS.seek empty @14:39:24.38 Forearm.reconfigure LooseForearm @14:39:24.42 Hand.grip @14:39:25.24 SE.seek down @14:39:25.36 Forearm.seek retract @14:39:26.38 Elbow.seek CC @14:39:27.00 SE.coast @14:39:28.56 Elbow.reconfigure LooseElbow @14:39:28.64 Forearm.reconfigure TightForearm @14:39:28.67 Forearm.seek CC @14:39:29.76 CC.seek closed @14:39:30.04 ATV.dial :air2 @14:39:30.40 AS.reconfigure ASslow1 @14:39:30.45 AS.seek 1.000ml @14:39:33.57 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 2.03 @14:39:33.58 Hand.release @14:39:34.38 Forearm.reconfigure LooseForearm @14:39:34.41 Forearm.seek retract @14:39:35.38 SC.coast @14:39:35.41 collectSample 1000ml w/bubble point of 55psi @14:39:35.43 Priming 7ml Sleepy.power :sampler,:ON @14:39:35.44 Intake.select :close @14:39:35.45 Exhaust.select :close @14:39:35.46 SS.hold @14:39:35.60 Calibrating pressure sensors @14:39:35.61 CTV.dial :air @14:39:36.95 SSV.dial :spit,avoiding => exhaust @14:39:36.96 SS.reconfigure SSfast @14:39:36.99 SS.seek 5.00ml @14:39:39.80 SSV.dial CBV @14:39:39.81 CBV.dial SSV @14:39:41.21 delay 3.8 seconds @14:39:45.10 SSV.dial :spit @14:39:46.44 SS.seek 0.00ml @14:39:49.13 SSV.dial CBV @14:39:50.46 CTV.dial :intake @14:39:51.24 Waiting for external sampler to finish priming... @14:39:51.25 delayUntil "14:49:35.43" @14:42:54.84 delay 5 seconds @14:42:59.85 ATV.dial ASV @14:43:00.67 ASV.dial :SPEdelivery @14:43:00.69 AS.seek 0.020ml @14:46:20.86 delay 5 seconds @14:46:25.89 ATV.dial :air2 @14:46:26.74 AS.seek 0.520ml @14:48:08.99 delay 5 seconds @14:48:14.00 ATV.dial ASV @14:48:14.79 ASV.dial :wasteR @14:48:15.11 AS.seek 0.300ml @14:49:00.23 delay 20 seconds @14:49:20.24 AS.seek 0.070ml @14:49:35.44 SSV.dial CBV @14:49:35.46 CBV.dial SSV @14:49:35.47 Allowing 55s for 7.0ml of remaining 7.0ml @14:49:35.48 Intake.select :open @14:49:37.61 Intake @36.6psia, vacuum from 20.0 to 24.0psi @14:49:37.63 SS.reconfigure forSampling @14:49:37.66 SS.seek 7.00ml @14:49:40.09 Vacuum too high -- waiting up to 40s @14:49:41.01 Intake @34.6psia, vacuum from 20.0 to 24.0psi @14:49:41.03 SS.reconfigure forSampling @14:49:41.06 SS.seek 7.00ml @14:49:43.46 Vacuum too high -- waiting up to 40s @14:49:44.83 Intake @34.4psia, vacuum from 20.0 to 24.0psi @14:49:44.85 SS.reconfigure forSampling @14:49:44.88 SS.seek 7.00ml @14:49:47.60 Stroke completed -- waiting for pressure to equalize ... @14:49:51.15 Intake.select :close @14:49:51.28 SSV.dial :exhaust @14:49:52.05 Exhaust.select :open @14:49:52.15 SS.reconfigure SSexhale @14:49:52.19 SS.seek 0.00ml @14:49:55.60 Exhaust.select :close @14:49:55.71 Sampling 1000.0ml @14:49:55.73 Intake.select :close Exhaust.select :close @14:49:55.74 SS.reconfigure SSconfig @14:49:55.77 SS.hold @14:49:55.92 CTV.dial :intake @14:49:55.94 SSV.dial CBV @14:49:55.96 CBV.dial SSV @14:49:56.73 Allowing 185s for 25.0ml of remaining 1000.0ml @14:49:56.74 Intake.select :open @14:49:58.87 Intake @35.7psia, vacuum from 20.0 to 24.0psi @14:49:58.89 SS.reconfigure forSampling @14:49:58.92 SS.seek 25.00ml @14:50:01.87 Vacuum too high -- waiting up to 40s @14:50:02.36 Intake @34.4psia, vacuum from 20.0 to 24.0psi @14:50:02.38 SS.reconfigure forSampling @14:50:02.41 SS.seek 25.00ml @14:50:04.52 Vacuum too high -- waiting up to 40s @14:50:05.01 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:50:05.03 SS.reconfigure forSampling @14:50:05.06 SS.seek 25.00ml @14:50:07.05 Vacuum too high -- waiting up to 40s @14:50:07.36 delay 20 seconds @14:50:07.54 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:50:07.56 SS.reconfigure forSampling @14:50:07.59 SS.seek 25.00ml @14:50:09.84 Vacuum too high -- waiting up to 40s @14:50:10.33 Intake @34.1psia, vacuum from 20.0 to 24.0psi @14:50:10.34 SS.reconfigure forSampling @14:50:10.38 SS.seek 25.00ml @14:50:18.87 Vacuum too high -- waiting up to 40s @14:50:19.35 Intake @34.7psia, vacuum from 20.0 to 24.0psi @14:50:19.37 SS.reconfigure forSampling @14:50:19.40 SS.seek 25.00ml @14:50:27.37 ATV.dial :waste2 @14:50:27.98 AS.reconfigure ASconfig @14:50:28.01 AS.seek empty @14:50:32.44 Elution @14:50:32.45 ATV.dial :air1 @14:50:33.41 AS.reconfigure ASslow1 @14:50:33.44 AS.seek 0.049ml @14:50:37.36 Stroke completed -- waiting for pressure to equalize ... @14:50:38.59 Intake.select :close @14:50:38.72 SSV.dial :exhaust @14:50:39.48 Exhaust.select :open @14:50:39.58 SS.reconfigure SSexhale @14:50:39.62 SS.seek 0.00ml @14:50:43.96 delay 3 seconds @14:50:46.97 ATV.dial :ElutionWater @14:50:47.54 AS.seek 0.109ml @14:50:48.47 Exhaust.select :close @14:50:48.57 SSV.dial CBV @14:50:48.59 CBV.dial SSV @14:50:49.38 Allowing 185s for 25.0ml of remaining 975.0ml @14:50:49.39 Intake.select :open @14:50:51.52 Intake @36.4psia, vacuum from 20.0 to 24.0psi @14:50:51.54 SS.reconfigure forSampling @14:50:51.57 SS.seek 25.00ml @14:51:00.05 delay 3 seconds @14:51:03.06 ATV.dial ASV,via => air2 @14:51:04.71 ASV.dial :SPEelution @14:51:05.38 AFV.select :carrierSolution @14:51:05.53 AS.reconfigure ASnorm @14:51:05.56 AS.seek 0.759ml @14:51:24.49 Vacuum too high -- waiting up to 40s @14:51:24.75 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:51:24.76 SS.reconfigure forSampling @14:51:24.80 SS.seek 25.00ml @14:51:35.18 Stroke completed -- waiting for pressure to equalize ... @14:51:35.82 Intake.select :close @14:51:35.96 SSV.dial :exhaust @14:51:36.73 Exhaust.select :open @14:51:36.83 SS.reconfigure SSexhale @14:51:36.87 SS.seek 0.00ml @14:51:39.10 delay 3 seconds @14:51:42.11 AFV.select :holdingCoil @14:51:42.25 AS.reconfigure ASslow1 @14:51:42.28 AS.seek 0.489ml @14:51:45.39 Exhaust.select :close @14:51:45.49 SSV.dial CBV @14:51:45.51 CBV.dial SSV @14:51:46.29 Allowing 185s for 25.0ml of remaining 950.0ml @14:51:46.31 Intake.select :open @14:51:48.44 Intake @35.4psia, vacuum from 20.0 to 24.0psi @14:51:48.46 SS.reconfigure forSampling @14:51:48.49 SS.seek 25.00ml @14:52:37.66 delay 5 seconds @14:52:42.72 AS.reconfigure ASslow2 @14:52:42.75 AS.seek 0.454ml @14:52:47.63 Stroke completed -- waiting for pressure to equalize ... @14:52:48.27 Intake.select :close @14:52:48.40 SSV.dial :exhaust @14:52:49.17 Exhaust.select :open @14:52:49.27 SS.reconfigure SSexhale @14:52:49.31 SS.seek 0.00ml @14:52:57.77 Exhaust.select :close @14:52:57.87 SSV.dial CBV @14:52:57.89 CBV.dial SSV @14:52:58.68 Allowing 185s for 25.0ml of remaining 925.0ml @14:52:58.69 Intake.select :open @14:53:00.83 Intake @36.3psia, vacuum from 20.0 to 24.0psi @14:53:00.85 SS.reconfigure forSampling @14:53:00.88 SS.seek 25.00ml @14:53:18.73 delay 1:00 @14:54:00.00 Stroke completed -- waiting for pressure to equalize ... @14:54:00.64 Intake.select :close @14:54:00.78 SSV.dial :exhaust @14:54:01.55 Exhaust.select :open @14:54:02.14 SS.reconfigure SSexhale @14:54:02.17 SS.seek 0.00ml @14:54:11.29 Exhaust.select :close @14:54:11.39 SSV.dial CBV @14:54:11.41 CBV.dial SSV @14:54:12.20 Allowing 185s for 25.0ml of remaining 900.0ml @14:54:12.21 Intake.select :open @14:54:14.34 Intake @36.0psia, vacuum from 20.0 to 24.0psi @14:54:14.36 SS.reconfigure forSampling @14:54:14.39 SS.seek 25.00ml @14:54:18.74 pushing eluate to holding coil @14:54:18.80 AS.seek 0.294ml @14:55:13.28 Stroke completed -- waiting for pressure to equalize ... @14:55:13.92 Intake.select :close @14:55:14.06 SSV.dial :exhaust @14:55:14.83 Exhaust.select :open @14:55:14.93 SS.reconfigure SSexhale @14:55:14.97 SS.seek 0.00ml @14:55:23.67 Exhaust.select :close @14:55:23.77 SSV.dial CBV @14:55:23.79 CBV.dial SSV @14:55:24.59 Allowing 185s for 25.0ml of remaining 875.0ml @14:55:24.60 Intake.select :open @14:55:26.74 Intake @35.8psia, vacuum from 20.0 to 24.0psi @14:55:26.75 SS.reconfigure forSampling @14:55:26.78 SS.seek 25.00ml @14:56:25.87 Stroke completed -- waiting for pressure to equalize ... @14:56:26.51 Intake.select :close @14:56:26.65 SSV.dial :exhaust @14:56:27.42 Exhaust.select :open @14:56:27.52 SS.reconfigure SSexhale @14:56:27.56 SS.seek 0.00ml @14:56:36.25 Exhaust.select :close @14:56:36.35 SSV.dial CBV @14:56:36.37 CBV.dial SSV @14:56:37.16 Allowing 185s for 25.0ml of remaining 850.0ml @14:56:37.17 Intake.select :open @14:56:39.30 Intake @36.0psia, vacuum from 20.0 to 24.0psi @14:56:39.32 SS.reconfigure forSampling @14:56:39.35 SS.seek 25.00ml @14:57:02.44 delay 30 seconds @14:57:32.45 ATV.dial :waste,via => air3 @14:57:33.91 AS.reconfigure ASconfig @14:57:33.94 AS.seek empty @14:57:38.45 Stroke completed -- waiting for pressure to equalize ... @14:57:39.09 Intake.select :close @14:57:39.23 SSV.dial :exhaust @14:57:39.99 Exhaust.select :open @14:57:40.09 SS.reconfigure SSexhale @14:57:40.13 SS.seek 0.00ml @14:57:49.16 Exhaust.select :close @14:57:49.26 SSV.dial CBV @14:57:49.28 CBV.dial SSV @14:57:50.08 Allowing 185s for 25.0ml of remaining 825.0ml @14:57:50.09 Intake.select :open @14:57:51.43 Parking Bleach in LysateSource line and SPEcolumn @14:57:51.44 ATV.dial :air3 @14:57:52.01 AS.reconfigure ASslow1 @14:57:52.04 AS.seek 0.029ml @14:57:52.22 Intake @36.1psia, vacuum from 20.0 to 24.0psi @14:57:52.24 SS.reconfigure forSampling @14:57:52.27 SS.seek 25.00ml @14:57:58.46 ATV.dial :ElutionWater @14:57:58.94 AS.seek 0.059ml @14:58:05.30 ATV.dial :air3 @14:58:05.68 AS.seek 0.069ml @14:58:07.97 delay 2 seconds @14:58:09.98 ATV.dial :bleach @14:58:10.44 AS.seek 0.711ml @14:58:51.41 Stroke completed -- waiting for pressure to equalize ... @14:58:52.05 Intake.select :close @14:58:52.18 SSV.dial :exhaust @14:58:52.96 Exhaust.select :open @14:58:53.06 SS.reconfigure SSexhale @14:58:53.10 SS.seek 0.00ml @14:59:02.30 Exhaust.select :close @14:59:02.40 SSV.dial CBV @14:59:02.42 CBV.dial SSV @14:59:03.22 Allowing 185s for 25.0ml of remaining 800.0ml @14:59:03.23 Intake.select :open @14:59:05.36 Intake @35.5psia, vacuum from 20.0 to 24.0psi @14:59:05.38 SS.reconfigure forSampling @14:59:05.41 SS.seek 25.00ml @15:00:04.56 Stroke completed -- waiting for pressure to equalize ... @15:00:05.20 Intake.select :close @15:00:05.33 SSV.dial :exhaust @15:00:06.09 Exhaust.select :open @15:00:06.19 SS.reconfigure SSexhale @15:00:06.23 SS.seek 0.00ml @15:00:15.33 Exhaust.select :close @15:00:15.43 SSV.dial CBV @15:00:15.45 CBV.dial SSV @15:00:16.23 Allowing 185s for 25.0ml of remaining 775.0ml @15:00:16.24 Intake.select :open @15:00:18.38 Intake @35.3psia, vacuum from 20.0 to 24.0psi @15:00:18.40 SS.reconfigure forSampling @15:00:18.43 SS.seek 25.00ml @15:00:21.67 delay 3 seconds @15:00:24.68 ATV.dial :air3 @15:00:25.00 delay 3 seconds @15:00:28.06 AS.seek 0.736ml @15:00:33.41 delay 3 seconds @15:00:36.42 ATV.dial :bleach @15:00:36.81 AS.seek 0.721ml @15:00:40.09 delay 3 seconds @15:00:43.10 ATV.dial :AIV @15:00:44.75 AS.reconfigure ASconfig @15:00:44.78 AS.seek 0.015ml @15:01:17.61 Stroke completed -- waiting for pressure to equalize ... @15:01:18.25 Intake.select :close @15:01:18.39 SSV.dial :exhaust @15:01:19.17 Exhaust.select :open @15:01:19.27 SS.reconfigure SSexhale @15:01:19.31 SS.seek 0.00ml @15:01:26.23 ATV.dial :waste @15:01:27.62 AS.seek empty @15:01:28.04 Exhaust.select :close @15:01:28.14 SSV.dial CBV @15:01:28.16 CBV.dial SSV @15:01:28.83 ATV.dial :air3 @15:01:28.96 Allowing 185s for 25.0ml of remaining 750.0ml @15:01:28.97 Intake.select :open @15:01:29.41 AS.reconfigure ASslow1 @15:01:29.45 AS.seek 0.009ml @15:01:31.10 Intake @36.0psia, vacuum from 20.0 to 24.0psi @15:01:31.12 SS.reconfigure forSampling @15:01:31.15 SS.seek 25.00ml @15:01:31.79 delay 2 seconds @15:01:33.80 ATV.dial :bleach @15:01:34.17 AS.seek 0.579ml @15:02:30.18 Stroke completed -- waiting for pressure to equalize ... @15:02:30.82 Intake.select :close @15:02:30.96 SSV.dial :exhaust @15:02:31.73 Exhaust.select :open @15:02:31.83 SS.reconfigure SSexhale @15:02:31.87 SS.seek 0.00ml @15:02:40.62 Exhaust.select :close @15:02:40.72 SSV.dial CBV @15:02:40.74 CBV.dial SSV @15:02:41.52 Allowing 185s for 25.0ml of remaining 725.0ml @15:02:41.53 Intake.select :open @15:02:43.67 Intake @35.7psia, vacuum from 20.0 to 24.0psi @15:02:43.69 SS.reconfigure forSampling @15:02:43.72 SS.seek 25.00ml @15:03:30.68 delay 3 seconds @15:03:33.69 ATV.dial :air3 @15:03:34.04 delay 3 seconds @15:03:37.10 AS.seek 0.604ml @15:03:42.51 delay 3 seconds @15:03:42.82 Stroke completed -- waiting for pressure to equalize ... @15:03:43.46 Intake.select :close @15:03:43.59 SSV.dial :exhaust @15:03:44.36 Exhaust.select :open @15:03:44.46 SS.reconfigure SSexhale @15:03:44.50 SS.seek 0.00ml @15:03:45.52 ATV.dial :bleach @15:03:45.92 AS.seek 0.589ml @15:03:49.23 delay 3 seconds @15:03:52.24 ATV.dial :ASV @15:03:53.13 ASV.dial :wasteR @15:03:53.80 AS.seek 0.000ml @15:03:53.99 Exhaust.select :close @15:03:54.09 SSV.dial CBV @15:03:54.11 CBV.dial SSV @15:03:54.90 Allowing 185s for 25.0ml of remaining 700.0ml @15:03:54.91 Intake.select :open @15:03:57.05 Intake @35.8psia, vacuum from 20.0 to 24.0psi @15:03:57.07 SS.reconfigure forSampling @15:03:57.10 SS.seek 25.00ml @15:04:56.12 Stroke completed -- waiting for pressure to equalize ... @15:04:56.76 Intake.select :close @15:04:56.89 SSV.dial :exhaust @15:04:57.67 Exhaust.select :open @15:04:57.77 SS.reconfigure SSexhale @15:04:57.81 SS.seek 0.00ml @15:05:06.57 Exhaust.select :close @15:05:06.67 SSV.dial CBV @15:05:06.69 CBV.dial SSV @15:05:07.48 Allowing 185s for 25.0ml of remaining 675.0ml @15:05:07.49 Intake.select :open @15:05:09.63 Intake @35.6psia, vacuum from 20.0 to 24.0psi @15:05:09.65 SS.reconfigure forSampling @15:05:09.68 SS.seek 25.00ml @15:05:54.19 delay 1:00 @15:06:08.77 Stroke completed -- waiting for pressure to equalize ... @15:06:09.41 Intake.select :close @15:06:09.54 SSV.dial :exhaust @15:06:10.30 Exhaust.select :open @15:06:10.40 SS.reconfigure SSexhale @15:06:10.44 SS.seek 0.00ml @15:06:19.27 Exhaust.select :close @15:06:19.37 SSV.dial CBV @15:06:19.39 CBV.dial SSV @15:06:20.18 Allowing 185s for 25.0ml of remaining 650.0ml @15:06:20.19 Intake.select :open @15:06:22.33 Intake @36.1psia, vacuum from 20.0 to 24.0psi @15:06:22.35 SS.reconfigure forSampling @15:06:22.38 SS.seek 25.00ml @15:06:54.20 AFV.select :carrierSolution @15:06:54.30 delay 2 seconds @15:06:56.31 AS.reconfigure ASconfig @15:06:56.34 AS.seek 1.000ml @15:07:22.25 Stroke completed -- waiting for pressure to equalize ... @15:07:22.89 Intake.select :close @15:07:23.02 SSV.dial :exhaust @15:07:23.78 Exhaust.select :open @15:07:23.88 SS.reconfigure SSexhale @15:07:23.92 SS.seek 0.00ml @15:07:32.89 Exhaust.select :close @15:07:32.99 SSV.dial CBV @15:07:33.01 CBV.dial SSV @15:07:33.80 Allowing 185s for 25.0ml of remaining 625.0ml @15:07:33.81 Intake.select :open @15:07:36.47 Intake @35.6psia, vacuum from 20.0 to 24.0psi @15:07:36.49 SS.reconfigure forSampling @15:07:36.52 SS.seek 25.00ml @15:07:54.95 delay 1 second @15:07:55.96 AFV.select :holdingCoil @15:07:56.06 delay 1 second @15:07:57.07 ATV.dial :ASV @15:07:57.08 ASV.dial :wasteR @15:07:57.09 AS.reconfigure ASslow1 @15:07:57.12 AS.seek 0.000ml @15:08:09.85 Vacuum too high -- waiting up to 40s @15:08:10.11 Intake @34.4psia, vacuum from 20.0 to 24.0psi @15:08:10.12 SS.reconfigure forSampling @15:08:10.16 SS.seek 25.00ml @15:08:58.53 Stroke completed -- waiting for pressure to equalize ... @15:08:59.17 Intake.select :close @15:08:59.31 SSV.dial :exhaust @15:09:00.08 Exhaust.select :open @15:09:00.18 SS.reconfigure SSexhale @15:09:00.22 SS.seek 0.00ml @15:09:09.18 Exhaust.select :close @15:09:09.28 SSV.dial CBV @15:09:09.30 CBV.dial SSV @15:09:10.09 Allowing 185s for 25.0ml of remaining 600.0ml @15:09:10.10 Intake.select :open @15:09:12.24 Intake @36.5psia, vacuum from 20.0 to 24.0psi @15:09:12.26 SS.reconfigure forSampling @15:09:12.29 SS.seek 25.00ml @15:11:02.46 Stroke completed -- waiting for pressure to equalize ... @15:11:03.69 Intake.select :close @15:11:03.82 SSV.dial :exhaust @15:11:04.59 Exhaust.select :open @15:11:04.69 SS.reconfigure SSexhale @15:11:04.73 SS.seek 0.00ml @15:11:13.32 Exhaust.select :close @15:11:13.42 SSV.dial CBV @15:11:13.44 CBV.dial SSV @15:11:14.24 Allowing 185s for 25.0ml of remaining 575.0ml @15:11:14.25 Intake.select :open @15:11:16.38 Intake @36.0psia, vacuum from 20.0 to 24.0psi @15:11:16.40 SS.reconfigure forSampling @15:11:16.43 SS.seek 25.00ml @15:11:21.39 delay 20 seconds @15:11:41.40 ATV.dial :air3 @15:11:42.52 AS.seek 0.590ml @15:13:07.50 Stroke completed -- waiting for pressure to equalize ... @15:13:08.14 Intake.select :close @15:13:08.28 SSV.dial :exhaust @15:13:09.05 Exhaust.select :open @15:13:09.15 SS.reconfigure SSexhale @15:13:09.19 SS.seek 0.00ml @15:13:18.02 Exhaust.select :close @15:13:18.12 SSV.dial CBV @15:13:18.14 CBV.dial SSV @15:13:18.92 Allowing 185s for 25.0ml of remaining 550.0ml @15:13:18.93 Intake.select :open @15:13:21.07 Intake @36.4psia, vacuum from 20.0 to 24.0psi @15:13:21.09 SS.reconfigure forSampling @15:13:21.12 SS.seek 25.00ml @15:13:43.17 delay 3 seconds @15:13:46.18 ATV.dial :ASV @15:13:47.36 ASV.dial :wasteR @15:13:47.42 AS.seek 0.090ml @15:15:11.53 Stroke completed -- waiting for pressure to equalize ... @15:15:12.75 Intake.select :close @15:15:12.89 SSV.dial :exhaust @15:15:13.67 Exhaust.select :open @15:15:13.77 SS.reconfigure SSexhale @15:15:13.81 SS.seek 0.00ml @15:15:22.87 Exhaust.select :close @15:15:22.97 SSV.dial CBV @15:15:22.99 CBV.dial SSV @15:15:23.77 Allowing 185s for 25.0ml of remaining 525.0ml @15:15:23.78 Intake.select :open @15:15:25.92 Intake @36.0psia, vacuum from 20.0 to 24.0psi @15:15:25.94 SS.reconfigure forSampling @15:15:25.97 SS.seek 25.00ml @15:15:29.63 delay 30 seconds @15:15:59.64 ATV.dial :extSrc @15:16:00.24 delay 3 seconds @15:16:03.25 ATV.dial :ElutionWater @15:16:04.18 AS.seek 0.120ml @15:16:10.57 ATV.dial :air3 @15:16:10.95 AS.seek 0.130ml @15:16:13.24 delay 2 seconds @15:16:15.25 ATV.dial :bleach @15:16:15.62 AS.seek 0.230ml @15:16:36.26 delay 3 seconds @15:16:39.27 ATV.dial :air3 @15:16:39.62 delay 3 seconds @15:16:42.68 AS.seek 0.255ml @15:16:48.02 delay 3 seconds @15:16:51.03 ATV.dial :bleach @15:16:51.42 AS.seek 0.240ml @15:16:54.73 delay 3 seconds @15:16:57.74 ATV.dial :ASV @15:16:58.62 ASV.dial :SPEdelivery @15:16:59.10 AFV.select :carrierSolution @15:16:59.20 delay 2 seconds @15:17:01.21 AS.reconfigure ASconfig @15:17:01.24 AS.seek 0.500ml @15:17:16.19 Stroke completed -- waiting for pressure to equalize ... @15:17:16.69 delay 1 second @15:17:17.70 AFV.select :holdingCoil @15:17:17.85 AS.reconfigure ASslow1 @15:17:17.89 AS.seek 0.170ml @15:17:18.60 Intake.select :close @15:17:18.73 SSV.dial :exhaust @15:17:19.50 Exhaust.select :open @15:17:19.60 SS.reconfigure SSexhale @15:17:19.64 SS.seek 0.00ml @15:17:28.17 Exhaust.select :close @15:17:28.27 SSV.dial CBV @15:17:28.29 CBV.dial SSV @15:17:29.08 Allowing 185s for 25.0ml of remaining 500.0ml @15:17:29.10 Intake.select :open @15:17:31.23 Intake @35.6psia, vacuum from 20.0 to 24.0psi @15:17:31.25 SS.reconfigure forSampling @15:17:31.28 SS.seek 25.00ml @15:18:25.49 SPE.hold 2:46:40,95.0C @15:18:25.51 delay 3:00 @15:18:34.10 Vacuum too high -- waiting up to 40s @15:18:34.36 Intake @33.8psia, vacuum from 20.0 to 24.0psi @15:18:34.38 SS.reconfigure forSampling @15:18:34.41 SS.seek 25.00ml @15:19:12.97 Vacuum too high -- waiting up to 40s @15:19:13.23 Intake @33.5psia, vacuum from 20.0 to 24.0psi @15:19:13.25 SS.reconfigure forSampling @15:19:13.28 SS.seek 25.00ml @15:19:16.91 Vacuum too high -- waiting up to 40s @15:19:17.17 Intake @33.3psia, vacuum from 20.0 to 24.0psi @15:19:17.18 SS.reconfigure forSampling @15:19:17.22 SS.seek 25.00ml @15:19:23.17 Stroke completed -- waiting for pressure to equalize ... @15:19:24.97 Intake.select :close @15:19:25.10 SSV.dial :exhaust @15:19:25.90 Exhaust.select :open @15:19:26.00 SS.reconfigure SSexhale @15:19:26.04 SS.seek 0.00ml @15:19:34.84 Exhaust.select :close @15:19:34.94 SSV.dial CBV @15:19:34.96 CBV.dial SSV @15:19:35.78 Allowing 185s for 25.0ml of remaining 475.0ml @15:19:35.79 Intake.select :open @15:19:37.93 Intake @33.6psia, vacuum from 20.0 to 24.0psi @15:19:37.95 SS.reconfigure forSampling @15:19:37.98 SS.seek 25.00ml @15:19:47.84 Vacuum too high -- waiting up to 40s @15:19:48.11 Intake @33.3psia, vacuum from 20.0 to 24.0psi @15:19:48.12 SS.reconfigure forSampling @15:19:48.16 SS.seek 25.00ml @15:19:52.53 Vacuum too high -- waiting up to 40s @15:19:52.79 Intake @33.4psia, vacuum from 20.0 to 24.0psi @15:19:52.80 SS.reconfigure forSampling @15:19:52.84 SS.seek 25.00ml @15:20:01.25 Vacuum too high -- waiting up to 40s @15:20:01.51 Intake @33.0psia, vacuum from 20.0 to 24.0psi @15:20:01.52 SS.reconfigure forSampling @15:20:01.56 SS.seek 25.00ml @15:20:12.03 Vacuum too high -- waiting up to 40s @15:20:12.29 Intake @33.0psia, vacuum from 20.0 to 24.0psi @15:20:12.30 SS.reconfigure forSampling @15:20:12.34 SS.seek 25.00ml @15:20:15.56 Vacuum too high -- waiting up to 40s @15:20:15.83 SS.seek 25.00ml @15:20:19.53 Vacuum too high -- waiting up to 40s @15:20:19.79 Intake @32.8psia, vacuum from 20.0 to 24.0psi @15:20:19.81 SS.reconfigure forSampling @15:20:19.84 SS.seek 25.00ml @15:20:24.65 Vacuum too high -- waiting up to 40s @15:20:24.93 SS.seek 25.00ml @15:20:27.65 Vacuum too high -- waiting up to 40s @15:20:27.92 Intake @32.7psia, vacuum from 20.0 to 24.0psi @15:20:27.94 SS.reconfigure forSampling @15:20:27.97 SS.seek 25.00ml @15:20:30.44 Vacuum too high -- waiting up to 40s @15:20:30.71 Intake @32.8psia, vacuum from 20.0 to 24.0psi @15:20:30.73 SS.reconfigure forSampling @15:20:30.76 SS.seek 25.00ml @15:20:32.78 Vacuum too high -- waiting up to 40s @15:20:33.05 Intake @32.6psia, vacuum from 20.0 to 24.0psi @15:20:33.07 SS.reconfigure forSampling @15:20:33.10 SS.seek 25.00ml @15:20:35.94 Vacuum too high -- waiting up to 40s @15:20:36.21 Intake @32.5psia, vacuum from 20.0 to 24.0psi @15:20:36.23 SS.reconfigure forSampling @15:20:36.26 SS.seek 25.00ml @15:20:39.30 Vacuum too high -- waiting up to 40s @15:20:39.57 Intake @32.9psia, vacuum from 20.0 to 24.0psi @15:20:39.58 SS.reconfigure forSampling @15:20:39.62 SS.seek 25.00ml @15:20:41.50 Vacuum too high -- waiting up to 40s @15:20:41.77 Intake @32.8psia, vacuum from 20.0 to 24.0psi @15:20:41.78 SS.reconfigure forSampling @15:20:41.82 SS.seek 25.00ml @15:20:45.86 Vacuum too high -- waiting up to 40s @15:20:46.14 SS.seek 25.00ml @15:20:48.28 Vacuum too high -- waiting up to 40s @15:20:48.55 Intake @32.8psia, vacuum from 20.0 to 24.0psi @15:20:48.57 SS.reconfigure forSampling @15:20:48.60 SS.seek 25.00ml @15:20:50.56 Vacuum too high -- waiting up to 40s @15:20:50.83 Intake @32.7psia, vacuum from 20.0 to 24.0psi @15:20:50.85 SS.reconfigure forSampling @15:20:50.88 SS.seek 25.00ml @15:20:53.81 Vacuum too high -- waiting up to 40s @15:20:54.08 Intake @33.6psia, vacuum from 20.0 to 24.0psi @15:20:54.10 SS.reconfigure forSampling @15:20:54.13 SS.seek 25.00ml @15:20:55.78 Vacuum too high -- waiting up to 40s @15:20:56.05 Intake @32.9psia, vacuum from 20.0 to 24.0psi @15:20:56.07 SS.reconfigure forSampling @15:20:56.10 SS.seek 25.00ml @15:20:58.87 Vacuum too high -- waiting up to 40s @15:20:59.14 Intake @32.9psia, vacuum from 20.0 to 24.0psi @15:20:59.16 SS.reconfigure forSampling @15:20:59.19 SS.seek 25.00ml @15:21:01.40 Vacuum too high -- waiting up to 40s @15:21:01.67 Intake @33.0psia, vacuum from 20.0 to 24.0psi @15:21:01.69 SS.reconfigure forSampling @15:21:01.72 SS.seek 25.00ml @15:21:03.90 Vacuum too high -- waiting up to 40s @15:21:04.17 Intake @32.8psia, vacuum from 20.0 to 24.0psi @15:21:04.19 SS.reconfigure forSampling @15:21:04.23 SS.seek 25.00ml @15:21:06.43 Vacuum too high -- waiting up to 40s @15:21:06.69 Intake @33.0psia, vacuum from 20.0 to 24.0psi @15:21:06.70 SS.reconfigure forSampling @15:21:06.74 SS.seek 25.00ml @15:21:08.71 Vacuum too high -- waiting up to 40s @15:21:08.98 Intake @33.0psia, vacuum from 20.0 to 24.0psi @15:21:09.00 SS.reconfigure forSampling @15:21:09.03 SS.seek 25.00ml @15:21:11.06 Vacuum too high -- waiting up to 40s @15:21:11.33 Intake @33.2psia, vacuum from 20.0 to 24.0psi @15:21:11.34 SS.reconfigure forSampling @15:21:11.38 SS.seek 25.00ml @15:21:13.21 Vacuum too high -- waiting up to 40s @15:21:13.48 Intake @33.0psia, vacuum from 20.0 to 24.0psi @15:21:13.50 SS.reconfigure forSampling @15:21:13.53 SS.seek 25.00ml @15:21:15.34 Vacuum too high -- waiting up to 40s @15:21:15.61 Intake @33.1psia, vacuum from 20.0 to 24.0psi @15:21:15.63 SS.reconfigure forSampling @15:21:15.66 SS.seek 25.00ml @15:21:17.53 Vacuum too high -- waiting up to 40s @15:21:17.80 Intake @32.7psia, vacuum from 20.0 to 24.0psi @15:21:17.81 SS.reconfigure forSampling @15:21:17.85 SS.seek 25.00ml @15:21:19.84 Vacuum too high -- waiting up to 40s @15:21:20.11 Intake @32.8psia, vacuum from 20.0 to 24.0psi @15:21:20.13 SS.reconfigure forSampling @15:21:20.16 SS.seek 25.00ml @15:21:22.43 Vacuum too high -- waiting up to 40s @15:21:22.70 Intake @33.0psia, vacuum from 20.0 to 24.0psi @15:21:22.71 SS.reconfigure forSampling @15:21:22.75 SS.seek 25.00ml @15:21:24.71 Vacuum too high -- waiting up to 40s @15:21:25.20 Intake @33.3psia, vacuum from 20.0 to 24.0psi @15:21:25.22 SS.reconfigure forSampling @15:21:25.25 SS.seek 25.00ml @15:21:25.52 AFV.select :carrierSolution @15:21:25.61 delay 2 seconds @15:21:27.43 Vacuum too high -- waiting up to 40s @15:21:27.67 AS.reconfigure ASconfig @15:21:27.70 AS.seek 1.000ml @15:21:27.92 Intake @33.2psia, vacuum from 20.0 to 24.0psi @15:21:27.94 SS.reconfigure forSampling @15:21:27.97 SS.seek 25.00ml @15:21:29.96 Vacuum too high -- waiting up to 40s @15:21:30.23 Intake @32.7psia, vacuum from 20.0 to 24.0psi @15:21:30.24 SS.reconfigure forSampling @15:21:30.28 SS.seek 25.00ml @15:21:32.28 Vacuum too high -- waiting up to 40s @15:21:32.55 Intake @32.8psia, vacuum from 20.0 to 24.0psi @15:21:32.56 SS.reconfigure forSampling @15:21:32.60 SS.seek 25.00ml @15:21:34.56 Vacuum too high -- waiting up to 40s @15:21:34.83 Intake @33.0psia, vacuum from 20.0 to 24.0psi @15:21:34.84 SS.reconfigure forSampling @15:21:34.88 SS.seek 25.00ml @15:21:36.74 Vacuum too high -- waiting up to 40s @15:21:37.59 Intake @33.5psia, vacuum from 20.0 to 24.0psi @15:21:37.60 SS.reconfigure forSampling @15:21:37.64 SS.seek 25.00ml @15:21:39.84 Vacuum too high -- waiting up to 40s @15:21:40.11 Intake @33.0psia, vacuum from 20.0 to 24.0psi @15:21:40.13 SS.reconfigure forSampling @15:21:40.16 SS.seek 25.00ml @15:21:42.03 Vacuum too high -- waiting up to 40s @15:21:42.30 Intake @32.9psia, vacuum from 20.0 to 24.0psi @15:21:42.31 SS.reconfigure forSampling @15:21:42.35 SS.seek 25.00ml @15:21:44.54 Vacuum too high -- waiting up to 40s @15:21:44.81 Intake @33.0psia, vacuum from 20.0 to 24.0psi @15:21:44.83 SS.reconfigure forSampling @15:21:44.86 SS.seek 25.00ml @15:21:46.82 Vacuum too high -- waiting up to 40s @15:21:47.09 Intake @33.0psia, vacuum from 20.0 to 24.0psi @15:21:47.11 SS.reconfigure forSampling @15:21:47.14 SS.seek 25.00ml @15:21:48.94 Vacuum too high -- waiting up to 40s @15:21:49.43 Intake @33.1psia, vacuum from 20.0 to 24.0psi @15:21:49.45 SS.reconfigure forSampling @15:21:49.48 SS.seek 25.00ml @15:21:50.31 Stroke completed -- waiting for pressure to equalize ... @15:21:52.72 Intake.select :close @15:21:52.85 SSV.dial :exhaust @15:21:53.65 Exhaust.select :open @15:21:53.75 SS.reconfigure SSexhale @15:21:53.79 SS.seek 0.00ml @15:22:02.73 Exhaust.select :close @15:22:02.83 SSV.dial CBV @15:22:02.85 CBV.dial SSV @15:22:03.68 Allowing 185s for 25.0ml of remaining 450.0ml @15:22:03.69 Intake.select :open @15:22:05.82 Intake @33.4psia, vacuum from 20.0 to 24.0psi @15:22:05.84 SS.reconfigure forSampling @15:22:05.87 SS.seek 25.00ml @15:22:08.63 Vacuum too high -- waiting up to 40s @15:22:08.90 Intake @33.0psia, vacuum from 20.0 to 24.0psi @15:22:08.92 SS.reconfigure forSampling @15:22:08.95 SS.seek 25.00ml @15:22:10.85 Vacuum too high -- waiting up to 40s @15:22:11.13 SS.seek 25.00ml @15:22:13.28 Vacuum too high -- waiting up to 40s @15:22:13.77 Intake @33.4psia, vacuum from 20.0 to 24.0psi @15:22:13.78 SS.reconfigure forSampling @15:22:13.82 SS.seek 25.00ml @15:22:15.88 Vacuum too high -- waiting up to 40s @15:22:16.15 Intake @33.5psia, vacuum from 20.0 to 24.0psi @15:22:16.16 SS.reconfigure forSampling @15:22:16.20 SS.seek 25.00ml @15:22:16.35 delay 1 second @15:22:17.36 AFV.select :holdingCoil @15:22:17.45 delay 1 second @15:22:17.88 Vacuum too high -- waiting up to 40s @15:22:18.15 Intake @33.3psia, vacuum from 20.0 to 24.0psi @15:22:18.17 SS.reconfigure forSampling @15:22:18.20 SS.seek 25.00ml @15:22:18.46 ASV.dial :wasteD @15:22:19.55 AS.seek 0.000ml @15:22:20.06 Vacuum too high -- waiting up to 40s @15:22:20.33 Intake @33.1psia, vacuum from 20.0 to 24.0psi @15:22:20.34 SS.reconfigure forSampling @15:22:20.38 SS.seek 25.00ml @15:22:22.22 Vacuum too high -- waiting up to 40s @15:22:22.49 Intake @33.0psia, vacuum from 20.0 to 24.0psi @15:22:22.51 SS.reconfigure forSampling @15:22:22.54 SS.seek 25.00ml @15:22:24.19 Vacuum too high -- waiting up to 40s @15:22:24.67 Intake @33.4psia, vacuum from 20.0 to 24.0psi @15:22:24.69 SS.reconfigure forSampling @15:22:24.72 SS.seek 25.00ml @15:22:27.00 Vacuum too high -- waiting up to 40s @15:22:27.27 Intake @33.3psia, vacuum from 20.0 to 24.0psi @15:22:27.28 SS.reconfigure forSampling @15:22:27.32 SS.seek 25.00ml @15:22:29.26 Vacuum too high -- waiting up to 40s @15:22:29.53 Intake @32.9psia, vacuum from 20.0 to 24.0psi @15:22:29.55 SS.reconfigure forSampling @15:22:29.58 SS.seek 25.00ml @15:22:31.94 Vacuum too high -- waiting up to 40s @15:22:32.43 Intake @33.2psia, vacuum from 20.0 to 24.0psi @15:22:32.44 SS.reconfigure forSampling @15:22:32.48 SS.seek 25.00ml @15:22:34.75 Vacuum too high -- waiting up to 40s @15:22:35.56 Intake @33.5psia, vacuum from 20.0 to 24.0psi @15:22:35.58 SS.reconfigure forSampling @15:22:35.61 SS.seek 25.00ml @15:22:37.91 Vacuum too high -- waiting up to 40s @15:22:38.19 Intake @33.1psia, vacuum from 20.0 to 24.0psi @15:22:38.20 SS.reconfigure forSampling @15:22:38.23 SS.seek 25.00ml @15:22:40.09 Vacuum too high -- waiting up to 40s @15:22:40.58 Intake @33.5psia, vacuum from 20.0 to 24.0psi @15:22:40.60 SS.reconfigure forSampling @15:22:40.63 SS.seek 25.00ml @15:22:42.44 Vacuum too high -- waiting up to 40s @15:22:42.71 Intake @32.7psia, vacuum from 20.0 to 24.0psi @15:22:42.73 SS.reconfigure forSampling @15:22:42.76 SS.seek 25.00ml @15:22:44.75 Vacuum too high -- waiting up to 40s @15:22:45.24 Intake @32.8psia, vacuum from 20.0 to 24.0psi @15:22:45.26 SS.reconfigure forSampling @15:22:45.29 SS.seek 25.00ml @15:22:47.50 Vacuum too high -- waiting up to 40s @15:22:47.99 Intake @32.8psia, vacuum from 20.0 to 24.0psi @15:22:48.01 SS.reconfigure forSampling @15:22:48.04 SS.seek 25.00ml @15:22:50.22 Vacuum too high -- waiting up to 40s @15:22:50.71 Intake @32.9psia, vacuum from 20.0 to 24.0psi @15:22:50.73 SS.reconfigure forSampling @15:22:50.76 SS.seek 25.00ml @15:22:52.81 Vacuum too high -- waiting up to 40s @15:22:53.29 Intake @32.5psia, vacuum from 20.0 to 24.0psi @15:22:53.31 SS.reconfigure forSampling @15:22:53.34 SS.seek 25.00ml @15:22:55.38 Vacuum too high -- waiting up to 40s @15:22:55.65 Intake @31.9psia, vacuum from 20.0 to 24.0psi @15:22:55.66 SS.reconfigure forSampling @15:22:55.70 SS.seek 25.00ml @15:22:57.97 Vacuum too high -- waiting up to 40s @15:22:58.46 Intake @32.7psia, vacuum from 20.0 to 24.0psi @15:22:58.47 SS.reconfigure forSampling @15:22:58.51 SS.seek 25.00ml @15:23:00.59 Vacuum too high -- waiting up to 40s @15:23:01.08 Intake @32.9psia, vacuum from 20.0 to 24.0psi @15:23:01.09 SS.reconfigure forSampling @15:23:01.13 SS.seek 25.00ml @15:23:03.06 Vacuum too high -- waiting up to 40s @15:23:03.55 Intake @32.8psia, vacuum from 20.0 to 24.0psi @15:23:03.56 SS.reconfigure forSampling @15:23:03.60 SS.seek 25.00ml @15:23:05.59 Vacuum too high -- waiting up to 40s @15:23:06.08 SS.seek 25.00ml @15:23:08.06 Vacuum too high -- waiting up to 40s @15:23:08.56 SS.seek 25.00ml @15:23:10.41 Vacuum too high -- waiting up to 40s @15:23:10.89 Intake @32.5psia, vacuum from 20.0 to 24.0psi @15:23:10.91 SS.reconfigure forSampling @15:23:10.94 SS.seek 25.00ml @15:23:13.12 Vacuum too high -- waiting up to 40s @15:23:13.61 Intake @32.9psia, vacuum from 20.0 to 24.0psi @15:23:13.62 SS.reconfigure forSampling @15:23:13.66 SS.seek 25.00ml @15:23:15.62 Vacuum too high -- waiting up to 40s @15:23:16.11 Intake @32.5psia, vacuum from 20.0 to 24.0psi @15:23:16.12 SS.reconfigure forSampling @15:23:16.16 SS.seek 25.00ml @15:23:18.14 Priming Eluate @15:23:18.15 AFV.select :carrierSolution @15:23:18.24 delay 2 seconds @15:23:18.47 Vacuum too high -- waiting up to 40s @15:23:18.95 Intake @32.8psia, vacuum from 20.0 to 24.0psi @15:23:18.96 SS.reconfigure forSampling @15:23:19.00 SS.seek 25.00ml @15:23:20.25 AS.seek 0.500ml @15:23:20.84 Vacuum too high -- waiting up to 40s @15:23:21.33 Intake @32.5psia, vacuum from 20.0 to 24.0psi @15:23:21.35 SS.reconfigure forSampling @15:23:21.38 SS.seek 25.00ml @15:23:24.53 Vacuum too high -- waiting up to 40s @15:23:25.03 Intake @32.5psia, vacuum from 20.0 to 24.0psi @15:23:25.04 SS.reconfigure forSampling @15:23:25.07 SS.seek 25.00ml @15:23:27.40 Vacuum too high -- waiting up to 40s @15:23:27.89 Intake @33.2psia, vacuum from 20.0 to 24.0psi @15:23:27.91 SS.reconfigure forSampling @15:23:27.94 SS.seek 25.00ml @15:23:29.78 Vacuum too high -- waiting up to 40s @15:23:30.27 Intake @32.5psia, vacuum from 20.0 to 24.0psi @15:23:30.29 SS.reconfigure forSampling @15:23:30.32 SS.seek 25.00ml @15:23:32.53 Vacuum too high -- waiting up to 40s @15:23:33.02 Intake @32.6psia, vacuum from 20.0 to 24.0psi @15:23:33.04 SS.reconfigure forSampling @15:23:33.07 SS.seek 25.00ml @15:23:35.15 Vacuum too high -- waiting up to 40s @15:23:35.63 Intake @32.7psia, vacuum from 20.0 to 24.0psi @15:23:35.65 SS.reconfigure forSampling @15:23:35.68 SS.seek 25.00ml @15:23:37.97 Vacuum too high -- waiting up to 40s @15:23:38.46 Intake @32.7psia, vacuum from 20.0 to 24.0psi @15:23:38.47 SS.reconfigure forSampling @15:23:38.51 SS.seek 25.00ml @15:23:40.53 Vacuum too high -- waiting up to 40s @15:23:41.02 Intake @32.8psia, vacuum from 20.0 to 24.0psi @15:23:41.03 SS.reconfigure forSampling @15:23:41.07 SS.seek 25.00ml @15:23:43.22 Vacuum too high -- waiting up to 40s @15:23:43.71 Intake @32.9psia, vacuum from 20.0 to 24.0psi @15:23:43.72 SS.reconfigure forSampling @15:23:43.76 SS.seek 25.00ml @15:23:45.68 Vacuum too high -- waiting up to 40s @15:23:46.17 Intake @32.6psia, vacuum from 20.0 to 24.0psi @15:23:46.18 SS.reconfigure forSampling @15:23:46.22 SS.seek 25.00ml @15:23:48.47 Vacuum too high -- waiting up to 40s @15:23:48.96 Intake @33.1psia, vacuum from 20.0 to 24.0psi @15:23:48.98 SS.reconfigure forSampling @15:23:49.01 SS.seek 25.00ml @15:23:49.69 delay 1 second @15:23:50.72 Vacuum too high -- waiting up to 40s @15:23:50.73 AFV.select :holdingCoil @15:23:50.83 ATV.dial ASV @15:23:50.84 ASV.dial :SPErecovery @15:23:51.21 Intake @32.8psia, vacuum from 20.0 to 24.0psi @15:23:51.23 SS.reconfigure forSampling @15:23:51.26 SS.seek 25.00ml @15:23:51.52 Swish #1 of 2 @15:23:51.58 AS.reconfigure ASslow0 @15:23:51.61 AS.seek 0.600ml @15:23:53.31 Vacuum too high -- waiting up to 40s @15:23:53.80 Intake @32.5psia, vacuum from 20.0 to 24.0psi @15:23:53.82 SS.reconfigure forSampling @15:23:53.85 SS.seek 25.00ml @15:23:56.03 Vacuum too high -- waiting up to 40s @15:23:56.51 Intake @32.6psia, vacuum from 20.0 to 24.0psi @15:23:56.53 SS.reconfigure forSampling @15:23:56.56 SS.seek 25.00ml @15:23:58.81 Vacuum too high -- waiting up to 40s @15:23:59.31 Intake @33.0psia, vacuum from 20.0 to 24.0psi @15:23:59.32 SS.reconfigure forSampling @15:23:59.36 SS.seek 25.00ml @15:24:01.25 Vacuum too high -- waiting up to 40s @15:24:01.73 Intake @32.7psia, vacuum from 20.0 to 24.0psi @15:24:01.74 SS.reconfigure forSampling @15:24:01.77 SS.seek 25.00ml @15:24:02.19 AS.seek 0.500ml @15:24:04.00 Vacuum too high -- waiting up to 40s @15:24:04.50 Intake @32.8psia, vacuum from 20.0 to 24.0psi @15:24:04.52 SS.reconfigure forSampling @15:24:04.55 SS.seek 25.00ml @15:24:06.40 Vacuum too high -- waiting up to 40s @15:24:06.88 Intake @33.0psia, vacuum from 20.0 to 24.0psi @15:24:07.50 SS.reconfigure forSampling @15:24:07.54 SS.seek 25.00ml @15:24:10.00 Vacuum too high -- waiting up to 40s @15:24:10.49 Intake @32.7psia, vacuum from 20.0 to 24.0psi @15:24:10.51 SS.reconfigure forSampling @15:24:10.54 SS.seek 25.00ml @15:24:12.53 Vacuum too high -- waiting up to 40s @15:24:12.67 Swish #2 of 2 @15:24:12.77 AS.seek 0.600ml @15:24:13.11 Intake @33.1psia, vacuum from 20.0 to 24.0psi @15:24:13.13 SS.reconfigure forSampling @15:24:13.16 SS.seek 25.00ml @15:24:15.34 Vacuum too high -- waiting up to 40s @15:24:15.84 Intake @32.8psia, vacuum from 20.0 to 24.0psi @15:24:15.85 SS.reconfigure forSampling @15:24:15.88 SS.seek 25.00ml @15:24:17.96 Vacuum too high -- waiting up to 40s @15:24:18.45 Intake @32.6psia, vacuum from 20.0 to 24.0psi @15:24:18.47 SS.reconfigure forSampling @15:24:18.50 SS.seek 25.00ml @15:24:20.56 Vacuum too high -- waiting up to 40s @15:24:21.05 Intake @32.6psia, vacuum from 20.0 to 24.0psi @15:24:21.07 SS.reconfigure forSampling @15:24:21.10 SS.seek 25.00ml @15:24:23.18 Vacuum too high -- waiting up to 40s @15:24:23.35 AS.seek 0.500ml @15:24:23.74 Intake @32.7psia, vacuum from 20.0 to 24.0psi @15:24:23.76 SS.reconfigure forSampling @15:24:23.79 SS.seek 25.00ml @15:24:25.93 Vacuum too high -- waiting up to 40s @15:24:26.42 Intake @33.1psia, vacuum from 20.0 to 24.0psi @15:24:26.44 SS.reconfigure forSampling @15:24:26.47 SS.seek 25.00ml @15:24:28.28 Vacuum too high -- waiting up to 40s @15:24:28.78 SS.seek 25.00ml @15:24:30.68 Vacuum too high -- waiting up to 40s @15:24:31.17 Intake @32.8psia, vacuum from 20.0 to 24.0psi @15:24:31.18 SS.reconfigure forSampling @15:24:31.22 SS.seek 25.00ml @15:24:33.21 Vacuum too high -- waiting up to 40s @15:24:33.69 Intake @32.4psia, vacuum from 20.0 to 24.0psi @15:24:33.70 SS.reconfigure forSampling @15:24:33.74 SS.seek 25.00ml @15:24:33.83 delay 3 seconds @15:24:35.93 Vacuum too high -- waiting up to 40s @15:24:36.63 Intake @33.2psia, vacuum from 20.0 to 24.0psi @15:24:36.65 SS.reconfigure forSampling @15:24:36.68 SS.seek 25.00ml @15:24:36.89 AS.reconfigure ASslow1 @15:24:36.92 AS.seek 0.676ml @15:24:38.69 Stroke completed -- waiting for pressure to equalize ... @15:24:42.27 Intake.select :close @15:24:42.40 SSV.dial :exhaust @15:24:43.19 Exhaust.select :open @15:24:43.29 SS.reconfigure SSexhale @15:24:43.33 SS.seek 0.00ml @15:24:52.44 Exhaust.select :close @15:24:52.54 SSV.dial CBV @15:24:52.56 CBV.dial SSV @15:24:53.37 Allowing 185s for 25.0ml of remaining 425.0ml @15:24:53.38 Intake.select :open @15:24:55.51 Intake @32.8psia, vacuum from 20.0 to 24.0psi @15:24:55.53 SS.reconfigure forSampling @15:24:55.56 SS.seek 25.00ml @15:24:58.80 Vacuum too high -- waiting up to 40s @15:24:59.28 Intake @33.1psia, vacuum from 20.0 to 24.0psi @15:24:59.29 SS.reconfigure forSampling @15:24:59.33 SS.seek 25.00ml @15:25:01.24 Vacuum too high -- waiting up to 40s @15:25:01.73 Intake @32.8psia, vacuum from 20.0 to 24.0psi @15:25:01.74 SS.reconfigure forSampling @15:25:01.78 SS.seek 25.00ml @15:25:04.12 Vacuum too high -- waiting up to 40s @15:25:04.96 Intake @33.7psia, vacuum from 20.0 to 24.0psi @15:25:04.97 SS.reconfigure forSampling @15:25:05.00 SS.seek 25.00ml @15:25:07.12 Vacuum too high -- waiting up to 40s @15:25:07.61 Intake @33.1psia, vacuum from 20.0 to 24.0psi @15:25:07.63 SS.reconfigure forSampling @15:25:07.66 SS.seek 25.00ml @15:25:09.81 Vacuum too high -- waiting up to 40s @15:25:10.30 Intake @32.8psia, vacuum from 20.0 to 24.0psi @15:25:10.32 SS.reconfigure forSampling @15:25:10.35 SS.seek 25.00ml @15:25:12.49 Vacuum too high -- waiting up to 40s @15:25:13.18 delay 3 seconds @15:25:13.24 Intake @33.3psia, vacuum from 20.0 to 24.0psi @15:25:13.25 SS.reconfigure forSampling @15:25:13.29 SS.seek 25.00ml @15:25:15.40 Vacuum too high -- waiting up to 40s @15:25:15.89 Intake @33.1psia, vacuum from 20.0 to 24.0psi @15:25:15.91 SS.reconfigure forSampling @15:25:15.94 SS.seek 25.00ml @15:25:16.19 Eluate primed @15:25:16.20 ATV.dial :waste2 @15:25:16.74 AS.reconfigure ASnorm @15:25:16.77 AS.seek 0.000ml @15:25:17.93 Vacuum too high -- waiting up to 40s @15:25:18.42 Intake @33.1psia, vacuum from 20.0 to 24.0psi @15:25:18.44 SS.reconfigure forSampling @15:25:18.47 SS.seek 25.00ml @15:25:20.65 Vacuum too high -- waiting up to 40s @15:25:21.14 Intake @33.0psia, vacuum from 20.0 to 24.0psi @15:25:21.15 SS.reconfigure forSampling @15:25:21.19 SS.seek 25.00ml @15:25:23.34 Vacuum too high -- waiting up to 40s @15:25:23.83 Intake @33.1psia, vacuum from 20.0 to 24.0psi @15:25:23.85 SS.reconfigure forSampling @15:25:23.88 SS.seek 25.00ml @15:25:25.80 Vacuum too high -- waiting up to 40s @15:25:26.29 Intake @32.9psia, vacuum from 20.0 to 24.0psi @15:25:26.30 SS.reconfigure forSampling @15:25:26.34 SS.seek 25.00ml @15:25:28.52 Vacuum too high -- waiting up to 40s @15:25:29.22 Intake @33.3psia, vacuum from 20.0 to 24.0psi @15:25:29.24 SS.reconfigure forSampling @15:25:29.27 SS.seek 25.00ml @15:25:31.46 Vacuum too high -- waiting up to 40s @15:25:32.16 Intake @33.4psia, vacuum from 20.0 to 24.0psi @15:25:32.18 SS.reconfigure forSampling @15:25:32.21 SS.seek 25.00ml @15:25:34.43 Vacuum too high -- waiting up to 40s @15:25:34.91 Intake @32.8psia, vacuum from 20.0 to 24.0psi @15:25:34.92 SS.reconfigure forSampling @15:25:34.96 SS.seek 25.00ml @15:25:37.12 Vacuum too high -- waiting up to 40s @15:25:37.82 Intake @33.5psia, vacuum from 20.0 to 24.0psi @15:25:37.84 SS.reconfigure forSampling @15:25:37.87 SS.seek 25.00ml @15:25:39.90 Vacuum too high -- waiting up to 40s @15:25:40.39 Intake @33.0psia, vacuum from 20.0 to 24.0psi @15:25:40.40 SS.reconfigure forSampling @15:25:40.44 SS.seek 25.00ml @15:25:42.55 Vacuum too high -- waiting up to 40s @15:25:43.24 Intake @33.4psia, vacuum from 20.0 to 24.0psi @15:25:43.25 SS.reconfigure forSampling @15:25:43.29 SS.seek 25.00ml @15:25:45.40 Vacuum too high -- waiting up to 40s @15:25:46.09 Intake @33.2psia, vacuum from 20.0 to 24.0psi @15:25:46.10 SS.reconfigure forSampling @15:25:46.14 SS.seek 25.00ml @15:25:48.40 Vacuum too high -- waiting up to 40s @15:25:49.67 Intake @33.4psia, vacuum from 20.0 to 24.0psi @15:25:49.68 SS.reconfigure forSampling @15:25:49.71 SS.seek 25.00ml @15:25:51.66 SPE.coast @15:25:51.74 ASV.dial :SPErecovery @15:25:51.76 Setting up PCR for HF183em -- writing /var/log/esp/HF183em14feb1415h700ml.pcr @15:25:51.77 Building PCR Slug from ASV with MM2 mix, PP4 primer @15:25:51.78 Priming with carrier ATV.dial ARV @15:25:52.48 ARV.dial :carrier @15:25:52.55 Vacuum too high -- waiting up to 40s @15:25:52.84 AS.seek 0.500ml @15:25:53.26 Intake @33.8psia, vacuum from 20.0 to 24.0psi @15:25:53.28 SS.reconfigure forSampling @15:25:53.31 SS.seek 25.00ml @15:25:55.37 Vacuum too high -- waiting up to 40s @15:25:55.86 Intake @33.1psia, vacuum from 20.0 to 24.0psi @15:25:55.88 SS.reconfigure forSampling @15:25:55.91 SS.seek 25.00ml @15:25:58.02 Vacuum too high -- waiting up to 40s @15:25:58.73 Intake @33.4psia, vacuum from 20.0 to 24.0psi @15:25:58.75 SS.reconfigure forSampling @15:25:58.78 SS.seek 25.00ml @15:26:00.87 Vacuum too high -- waiting up to 40s @15:26:01.58 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:26:01.60 SS.reconfigure forSampling @15:26:01.63 SS.seek 25.00ml @15:26:03.68 Vacuum too high -- waiting up to 40s @15:26:04.17 Intake @33.1psia, vacuum from 20.0 to 24.0psi @15:26:04.18 SS.reconfigure forSampling @15:26:04.22 SS.seek 25.00ml @15:26:06.33 Vacuum too high -- waiting up to 40s @15:26:07.06 Intake @33.2psia, vacuum from 20.0 to 24.0psi @15:26:07.08 SS.reconfigure forSampling @15:26:07.11 SS.seek 25.00ml @15:26:09.24 Vacuum too high -- waiting up to 40s @15:26:09.95 Intake @33.1psia, vacuum from 20.0 to 24.0psi @15:26:09.96 SS.reconfigure forSampling @15:26:10.00 SS.seek 25.00ml @15:26:12.49 Vacuum too high -- waiting up to 40s @15:26:13.20 Intake @32.9psia, vacuum from 20.0 to 24.0psi @15:26:13.21 SS.reconfigure forSampling @15:26:13.25 SS.seek 25.00ml @15:26:15.65 Vacuum too high -- waiting up to 40s @15:26:16.34 Intake @33.1psia, vacuum from 20.0 to 24.0psi @15:26:16.35 SS.reconfigure forSampling @15:26:16.39 SS.seek 25.00ml @15:26:18.40 Vacuum too high -- waiting up to 40s @15:26:18.75 AFV.select :carrierSolution @15:26:18.90 delay 2 seconds @15:26:19.15 Intake @33.4psia, vacuum from 20.0 to 24.0psi @15:26:19.17 SS.reconfigure forSampling @15:26:19.20 SS.seek 25.00ml @15:26:20.91 AS.reconfigure ASconfig @15:26:20.95 AS.seek 1.000ml @15:26:21.36 Vacuum too high -- waiting up to 40s @15:26:22.06 Intake @33.8psia, vacuum from 20.0 to 24.0psi @15:26:22.08 SS.reconfigure forSampling @15:26:22.11 SS.seek 25.00ml @15:26:24.02 Vacuum too high -- waiting up to 40s @15:26:24.72 Intake @33.5psia, vacuum from 20.0 to 24.0psi @15:26:24.74 SS.reconfigure forSampling @15:26:24.77 SS.seek 25.00ml @15:26:26.96 Vacuum too high -- waiting up to 40s @15:26:27.66 Intake @33.4psia, vacuum from 20.0 to 24.0psi @15:26:27.67 SS.reconfigure forSampling @15:26:27.71 SS.seek 25.00ml @15:26:30.02 Vacuum too high -- waiting up to 40s @15:26:31.32 Intake @33.9psia, vacuum from 20.0 to 24.0psi @15:26:31.33 SS.reconfigure forSampling @15:26:31.36 SS.seek 25.00ml @15:26:33.74 Vacuum too high -- waiting up to 40s @15:26:34.50 Intake @33.4psia, vacuum from 20.0 to 24.0psi @15:26:34.52 SS.reconfigure forSampling @15:26:34.55 SS.seek 25.00ml @15:26:36.83 Vacuum too high -- waiting up to 40s @15:26:37.53 Intake @33.4psia, vacuum from 20.0 to 24.0psi @15:26:37.54 SS.reconfigure forSampling @15:26:37.58 SS.seek 25.00ml @15:26:39.77 Vacuum too high -- waiting up to 40s @15:26:40.48 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:26:40.50 SS.reconfigure forSampling @15:26:40.53 SS.seek 25.00ml @15:26:42.30 Vacuum too high -- waiting up to 40s @15:26:43.00 Intake @33.2psia, vacuum from 20.0 to 24.0psi @15:26:43.02 SS.reconfigure forSampling @15:26:43.05 SS.seek 25.00ml @15:26:45.68 Vacuum too high -- waiting up to 40s @15:26:46.38 Intake @33.7psia, vacuum from 20.0 to 24.0psi @15:26:46.39 SS.reconfigure forSampling @15:26:46.43 SS.seek 25.00ml @15:26:48.49 Vacuum too high -- waiting up to 40s @15:26:49.20 Intake @33.3psia, vacuum from 20.0 to 24.0psi @15:26:49.22 SS.reconfigure forSampling @15:26:49.25 SS.seek 25.00ml @15:26:50.36 delay 1 second @15:26:51.37 AFV.select :holdingCoil @15:26:51.46 Vacuum too high -- waiting up to 40s @15:26:51.48 delay 1 second @15:26:52.16 Intake @33.9psia, vacuum from 20.0 to 24.0psi @15:26:52.18 SS.reconfigure forSampling @15:26:52.21 SS.seek 25.00ml @15:26:52.49 ATV.dial :waste @15:26:53.77 AS.reconfigure ASnorm @15:26:53.80 AS.seek empty @15:26:53.86 Vacuum too high -- waiting up to 40s @15:26:54.58 Intake @33.9psia, vacuum from 20.0 to 24.0psi @15:26:54.59 SS.reconfigure forSampling @15:26:54.63 SS.seek 25.00ml @15:26:56.55 Vacuum too high -- waiting up to 40s @15:26:57.26 Intake @33.5psia, vacuum from 20.0 to 24.0psi @15:26:57.27 SS.reconfigure forSampling @15:26:57.31 SS.seek 25.00ml @15:26:59.36 Vacuum too high -- waiting up to 40s @15:27:00.06 Intake @33.4psia, vacuum from 20.0 to 24.0psi @15:27:00.08 SS.reconfigure forSampling @15:27:00.11 SS.seek 25.00ml @15:27:02.39 Vacuum too high -- waiting up to 40s @15:27:03.08 Intake @33.1psia, vacuum from 20.0 to 24.0psi @15:27:03.09 SS.reconfigure forSampling @15:27:03.13 SS.seek 25.00ml @15:27:05.30 Vacuum too high -- waiting up to 40s @15:27:06.22 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:27:06.23 SS.reconfigure forSampling @15:27:06.27 SS.seek 25.00ml @15:27:08.42 Vacuum too high -- waiting up to 40s @15:27:09.12 Intake @33.0psia, vacuum from 20.0 to 24.0psi @15:27:09.14 SS.reconfigure forSampling @15:27:09.17 SS.seek 25.00ml @15:27:11.52 Vacuum too high -- waiting up to 40s @15:27:12.23 Intake @33.0psia, vacuum from 20.0 to 24.0psi @15:27:12.84 SS.reconfigure forSampling @15:27:12.88 SS.seek 25.00ml @15:27:15.67 Vacuum too high -- waiting up to 40s @15:27:16.60 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:27:16.62 SS.reconfigure forSampling @15:27:16.65 SS.seek 25.00ml @15:27:18.74 Vacuum too high -- waiting up to 40s @15:27:19.45 Intake @33.3psia, vacuum from 20.0 to 24.0psi @15:27:19.47 SS.reconfigure forSampling @15:27:19.50 SS.seek 25.00ml @15:27:21.77 Vacuum too high -- waiting up to 40s @15:27:22.48 Intake @33.1psia, vacuum from 20.0 to 24.0psi @15:27:22.50 SS.reconfigure forSampling @15:27:22.53 SS.seek 25.00ml @15:27:24.80 Vacuum too high -- waiting up to 40s @15:27:25.74 SS.seek 25.00ml @15:27:28.20 Vacuum too high -- waiting up to 40s @15:27:29.12 Intake @33.5psia, vacuum from 20.0 to 24.0psi @15:27:29.13 SS.reconfigure forSampling @15:27:29.17 SS.seek 25.00ml @15:27:31.36 Vacuum too high -- waiting up to 40s @15:27:32.07 Intake @33.2psia, vacuum from 20.0 to 24.0psi @15:27:32.09 SS.reconfigure forSampling @15:27:32.12 SS.seek 25.00ml @15:27:34.11 Vacuum too high -- waiting up to 40s @15:27:34.82 Intake @33.0psia, vacuum from 20.0 to 24.0psi @15:27:34.84 SS.reconfigure forSampling @15:27:34.87 SS.seek 25.00ml @15:27:37.27 Vacuum too high -- waiting up to 40s @15:27:38.20 Intake @33.7psia, vacuum from 20.0 to 24.0psi @15:27:38.21 SS.reconfigure forSampling @15:27:38.25 SS.seek 25.00ml @15:27:40.36 Vacuum too high -- waiting up to 40s @15:27:41.07 Intake @33.5psia, vacuum from 20.0 to 24.0psi @15:27:41.09 SS.reconfigure forSampling @15:27:41.12 SS.seek 25.00ml @15:27:41.46 Stroke completed -- waiting for pressure to equalize ... @15:27:45.29 AFV.select :carrierSolution @15:27:45.40 delay 2 seconds @15:27:45.63 Intake.select :close @15:27:45.76 SSV.dial :exhaust @15:27:46.53 Exhaust.select :open @15:27:46.64 SS.reconfigure SSexhale @15:27:46.68 SS.seek 0.00ml @15:27:47.41 AS.reconfigure ASconfig @15:27:47.45 AS.seek 0.500ml @15:27:55.34 Exhaust.select :close @15:27:55.45 SSV.dial CBV @15:27:55.47 CBV.dial SSV @15:27:56.26 Allowing 185s for 25.0ml of remaining 400.0ml @15:27:56.28 Intake.select :open @15:27:58.41 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:27:58.43 SS.reconfigure forSampling @15:27:58.46 SS.seek 25.00ml @15:28:01.86 Vacuum too high -- waiting up to 40s @15:28:02.55 Intake @33.1psia, vacuum from 20.0 to 24.0psi @15:28:02.57 SS.reconfigure forSampling @15:28:02.60 SS.seek 25.00ml @15:28:05.00 Vacuum too high -- waiting up to 40s @15:28:05.71 Intake @33.3psia, vacuum from 20.0 to 24.0psi @15:28:05.73 SS.reconfigure forSampling @15:28:05.76 SS.seek 25.00ml @15:28:08.70 Vacuum too high -- waiting up to 40s @15:28:08.97 Intake @33.6psia, vacuum from 20.0 to 24.0psi @15:28:08.99 SS.reconfigure forSampling @15:28:09.02 SS.seek 25.00ml @15:28:11.23 Vacuum too high -- waiting up to 40s @15:28:12.16 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:28:12.18 SS.reconfigure forSampling @15:28:12.21 SS.seek 25.00ml @15:28:14.39 Vacuum too high -- waiting up to 40s @15:28:15.10 Intake @33.6psia, vacuum from 20.0 to 24.0psi @15:28:15.12 SS.reconfigure forSampling @15:28:15.15 SS.seek 25.00ml @15:28:16.92 delay 1 second @15:28:17.36 Vacuum too high -- waiting up to 40s @15:28:17.93 AFV.select :holdingCoil @15:28:18.09 Building Slug ATV.dial :ARV @15:28:18.13 Intake @33.5psia, vacuum from 20.0 to 24.0psi @15:28:18.15 SS.reconfigure forSampling @15:28:18.18 SS.seek 25.00ml @15:28:19.44 delay 2 seconds @15:28:20.30 Vacuum too high -- waiting up to 40s @15:28:21.23 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:28:21.24 SS.reconfigure forSampling @15:28:21.28 SS.seek 25.00ml @15:28:21.44 ARV.dial :air1 @15:28:21.89 delay 2 seconds @15:28:23.61 Vacuum too high -- waiting up to 40s @15:28:23.94 AS.reconfigure ASslow2 @15:28:23.97 AS.seek 0.506ml @15:28:24.36 Intake @33.3psia, vacuum from 20.0 to 24.0psi @15:28:24.38 SS.reconfigure forSampling @15:28:24.41 SS.seek 25.00ml @15:28:26.70 Vacuum too high -- waiting up to 40s @15:28:27.63 Intake @33.5psia, vacuum from 20.0 to 24.0psi @15:28:27.64 SS.reconfigure forSampling @15:28:27.68 SS.seek 25.00ml @15:28:30.08 Vacuum too high -- waiting up to 40s @15:28:30.33 delay 2 seconds @15:28:31.01 Intake @33.9psia, vacuum from 20.0 to 24.0psi @15:28:31.02 SS.reconfigure forSampling @15:28:31.06 SS.seek 25.00ml @15:28:32.34 ARV.dial :MM2 @15:28:33.54 Vacuum too high -- waiting up to 40s @15:28:33.68 delay 2 seconds @15:28:34.26 Intake @33.5psia, vacuum from 20.0 to 24.0psi @15:28:34.27 SS.reconfigure forSampling @15:28:34.30 SS.seek 25.00ml @15:28:35.68 Adding MM2 @15:28:35.74 AS.seek 0.524ml @15:28:36.48 Vacuum too high -- waiting up to 40s @15:28:37.41 Intake @33.4psia, vacuum from 20.0 to 24.0psi @15:28:37.42 SS.reconfigure forSampling @15:28:37.45 SS.seek 25.00ml @15:28:39.79 Vacuum too high -- waiting up to 40s @15:28:40.70 Intake @33.3psia, vacuum from 20.0 to 24.0psi @15:28:40.71 SS.reconfigure forSampling @15:28:40.74 SS.seek 25.00ml @15:28:43.42 Vacuum too high -- waiting up to 40s @15:28:44.33 Intake @34.1psia, vacuum from 20.0 to 24.0psi @15:28:44.34 SS.reconfigure forSampling @15:28:44.38 SS.seek 25.00ml @15:28:46.23 Vacuum too high -- waiting up to 40s @15:28:47.53 Intake @33.6psia, vacuum from 20.0 to 24.0psi @15:28:47.55 SS.reconfigure forSampling @15:28:47.58 SS.seek 25.00ml @15:28:50.36 Vacuum too high -- waiting up to 40s @15:28:51.29 Intake @33.8psia, vacuum from 20.0 to 24.0psi @15:28:51.31 SS.reconfigure forSampling @15:28:51.34 SS.seek 25.00ml @15:28:53.57 Vacuum too high -- waiting up to 40s @15:28:54.32 delay 25 seconds @15:28:54.51 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:28:54.53 SS.reconfigure forSampling @15:28:54.56 SS.seek 25.00ml @15:28:56.67 Vacuum too high -- waiting up to 40s @15:28:57.60 Intake @33.9psia, vacuum from 20.0 to 24.0psi @15:28:57.62 SS.reconfigure forSampling @15:28:57.65 SS.seek 25.00ml @15:28:59.95 Vacuum too high -- waiting up to 40s @15:29:00.66 Intake @33.5psia, vacuum from 20.0 to 24.0psi @15:29:00.68 SS.reconfigure forSampling @15:29:00.71 SS.seek 25.00ml @15:29:02.89 Vacuum too high -- waiting up to 40s @15:29:03.82 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:29:03.83 SS.reconfigure forSampling @15:29:03.86 SS.seek 25.00ml @15:29:05.79 Vacuum too high -- waiting up to 40s @15:29:06.48 Intake @33.3psia, vacuum from 20.0 to 24.0psi @15:29:06.50 SS.reconfigure forSampling @15:29:06.53 SS.seek 25.00ml @15:29:09.01 Vacuum too high -- waiting up to 40s @15:29:09.94 Intake @33.8psia, vacuum from 20.0 to 24.0psi @15:29:09.95 SS.reconfigure forSampling @15:29:09.99 SS.seek 25.00ml @15:29:12.17 Vacuum too high -- waiting up to 40s @15:29:13.09 Intake @33.1psia, vacuum from 20.0 to 24.0psi @15:29:13.11 SS.reconfigure forSampling @15:29:13.14 SS.seek 25.00ml @15:29:15.48 Vacuum too high -- waiting up to 40s @15:29:16.42 Intake @33.7psia, vacuum from 20.0 to 24.0psi @15:29:16.43 SS.reconfigure forSampling @15:29:16.46 SS.seek 25.00ml @15:29:18.54 Vacuum too high -- waiting up to 40s @15:29:19.33 ARV.dial :PP4 @15:29:19.50 Intake @33.8psia, vacuum from 20.0 to 24.0psi @15:29:19.51 SS.reconfigure forSampling @15:29:19.55 SS.seek 25.00ml @15:29:19.67 Adding PP4 @15:29:19.68 delay 2 seconds @15:29:21.73 AS.seek 0.530ml @15:29:21.89 Vacuum too high -- waiting up to 40s @15:29:22.83 SS.seek 25.00ml @15:29:24.79 Vacuum too high -- waiting up to 40s @15:29:25.71 Intake @33.4psia, vacuum from 20.0 to 24.0psi @15:29:25.72 SS.reconfigure forSampling @15:29:25.76 SS.seek 25.00ml @15:29:28.14 Vacuum too high -- waiting up to 40s @15:29:28.24 delay 10 seconds @15:29:29.73 Intake @33.9psia, vacuum from 20.0 to 24.0psi @15:29:29.75 SS.reconfigure forSampling @15:29:29.78 SS.seek 25.00ml @15:29:32.42 Vacuum too high -- waiting up to 40s @15:29:33.35 Intake @33.5psia, vacuum from 20.0 to 24.0psi @15:29:33.37 SS.reconfigure forSampling @15:29:33.40 SS.seek 25.00ml @15:29:35.79 Vacuum too high -- waiting up to 40s @15:29:36.72 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:29:36.74 SS.reconfigure forSampling @15:29:36.77 SS.seek 25.00ml @15:29:38.25 ARV.dial :air3 @15:29:38.63 Vacuum too high -- waiting up to 40s @15:29:38.67 adding ARV air @15:29:38.68 delay 2 seconds @15:29:39.35 Intake @33.5psia, vacuum from 20.0 to 24.0psi @15:29:39.36 SS.reconfigure forSampling @15:29:39.40 SS.seek 25.00ml @15:29:40.73 AS.seek 0.536ml @15:29:41.57 Vacuum too high -- waiting up to 40s @15:29:42.50 Intake @33.6psia, vacuum from 20.0 to 24.0psi @15:29:42.51 SS.reconfigure forSampling @15:29:42.55 SS.seek 25.00ml @15:29:44.88 Vacuum too high -- waiting up to 40s @15:29:45.80 Intake @33.7psia, vacuum from 20.0 to 24.0psi @15:29:45.81 SS.reconfigure forSampling @15:29:45.84 SS.seek 25.00ml @15:29:46.98 delay 5 seconds @15:29:47.92 Vacuum too high -- waiting up to 40s @15:29:48.85 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:29:48.87 SS.reconfigure forSampling @15:29:48.90 SS.seek 25.00ml @15:29:50.92 Vacuum too high -- waiting up to 40s @15:29:51.84 Intake @33.7psia, vacuum from 20.0 to 24.0psi @15:29:51.85 SS.reconfigure forSampling @15:29:51.89 SS.seek 25.00ml @15:29:51.99 ARV.dial :carrier @15:29:53.03 delay 7 seconds @15:29:53.95 Vacuum too high -- waiting up to 40s @15:29:54.87 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:29:54.88 SS.reconfigure forSampling @15:29:54.92 SS.seek 25.00ml @15:29:57.10 Vacuum too high -- waiting up to 40s @15:29:58.02 Intake @33.5psia, vacuum from 20.0 to 24.0psi @15:29:58.04 SS.reconfigure forSampling @15:29:58.07 SS.seek 25.00ml @15:30:00.08 AS.seek 0.594ml @15:30:00.48 Vacuum too high -- waiting up to 40s @15:30:01.62 Intake @34.1psia, vacuum from 20.0 to 24.0psi @15:30:01.63 SS.reconfigure forSampling @15:30:01.67 SS.seek 25.00ml @15:30:03.79 Vacuum too high -- waiting up to 40s @15:30:04.70 Intake @33.5psia, vacuum from 20.0 to 24.0psi @15:30:04.71 SS.reconfigure forSampling @15:30:04.74 SS.seek 25.00ml @15:30:07.23 Vacuum too high -- waiting up to 40s @15:30:08.80 Intake @33.4psia, vacuum from 20.0 to 24.0psi @15:30:08.82 SS.reconfigure forSampling @15:30:08.85 SS.seek 25.00ml @15:30:11.63 Vacuum too high -- waiting up to 40s @15:30:12.78 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:30:12.80 SS.reconfigure forSampling @15:30:12.83 SS.seek 25.00ml @15:30:15.13 Vacuum too high -- waiting up to 40s @15:30:16.06 Intake @33.6psia, vacuum from 20.0 to 24.0psi @15:30:16.08 SS.reconfigure forSampling @15:30:16.11 SS.seek 25.00ml @15:30:18.54 Vacuum too high -- waiting up to 40s @15:30:19.68 Intake @33.5psia, vacuum from 20.0 to 24.0psi @15:30:19.69 SS.reconfigure forSampling @15:30:19.73 SS.seek 25.00ml @15:30:22.32 Vacuum too high -- waiting up to 40s @15:30:23.45 Intake @33.9psia, vacuum from 20.0 to 24.0psi @15:30:23.46 SS.reconfigure forSampling @15:30:23.50 SS.seek 25.00ml @15:30:25.66 Vacuum too high -- waiting up to 40s @15:30:26.57 Intake @33.8psia, vacuum from 20.0 to 24.0psi @15:30:26.58 SS.reconfigure forSampling @15:30:26.62 SS.seek 25.00ml @15:30:28.60 Vacuum too high -- waiting up to 40s @15:30:29.53 Intake @33.2psia, vacuum from 20.0 to 24.0psi @15:30:29.54 SS.reconfigure forSampling @15:30:29.58 SS.seek 25.00ml @15:30:31.85 Vacuum too high -- waiting up to 40s @15:30:33.21 Intake @33.8psia, vacuum from 20.0 to 24.0psi @15:30:33.22 SS.reconfigure forSampling @15:30:33.26 SS.seek 25.00ml @15:30:35.82 Vacuum too high -- waiting up to 40s @15:30:36.72 Intake @33.3psia, vacuum from 20.0 to 24.0psi @15:30:36.73 SS.reconfigure forSampling @15:30:36.77 SS.seek 25.00ml @15:30:39.01 Vacuum too high -- waiting up to 40s @15:30:40.15 Intake @33.2psia, vacuum from 20.0 to 24.0psi @15:30:40.16 SS.reconfigure forSampling @15:30:40.20 SS.seek 25.00ml @15:30:42.85 Vacuum too high -- waiting up to 40s @15:30:44.18 Intake @34.1psia, vacuum from 20.0 to 24.0psi @15:30:44.19 SS.reconfigure forSampling @15:30:44.23 SS.seek 25.00ml @15:30:46.57 Vacuum too high -- waiting up to 40s @15:30:47.68 Intake @33.5psia, vacuum from 20.0 to 24.0psi @15:30:47.69 SS.reconfigure forSampling @15:30:47.73 SS.seek 25.00ml @15:30:50.44 Vacuum too high -- waiting up to 40s @15:30:51.81 Intake @33.7psia, vacuum from 20.0 to 24.0psi @15:30:51.82 SS.reconfigure forSampling @15:30:51.85 SS.seek 25.00ml @15:30:54.38 Vacuum too high -- waiting up to 40s @15:30:55.53 Intake @33.7psia, vacuum from 20.0 to 24.0psi @15:30:55.55 SS.reconfigure forSampling @15:30:55.58 SS.seek 25.00ml @15:30:56.98 Stroke completed -- waiting for pressure to equalize ... @15:30:59.54 delay 5 seconds @15:31:01.27 SS.reconfigure SSconfig @15:31:01.31 SS.hold @15:31:01.40 Intake.select :close @15:31:01.55 ShallowSampler::Clogged in WCR -- progress too slow after 625.0ml @15:31:01.56 SSV.dial :exhaust @15:31:02.33 Exhaust.select :open @15:31:02.43 SS.reconfigure SSexhale @15:31:02.47 SS.seek 0.00ml @15:31:04.55 ATV.dial :ASV @15:31:04.89 delay 2 seconds @15:31:06.94 AS.seek 0.600ml @15:31:10.94 Exhaust.select :close @15:31:11.06 Sampled 625.0ml @15:31:11.07 collectSample: Complete @15:31:11.08 Drying puck before fixing for 04:00.00 @15:31:11.09 CBV.dial :waste2b @15:31:11.85 delay 1:00 @15:31:13.58 delay 4 seconds @15:31:14.05 Sleepy.power :sampler,:OFF @15:31:17.59 ATV.dial ARV @15:31:17.93 delay 2 seconds @15:31:19.94 ARV.dial :carrier @15:31:19.95 delay 2 seconds @15:31:22.00 AS.seek 0.650ml @15:32:11.85 CSV.dial :air @15:32:12.31 CS.reconfigure CSfast @15:32:12.34 CS.seek 10.000ml @15:32:13.36 delay 4 seconds @15:32:17.37 ATV.dial PCR @15:32:17.88 ASV.dial :waste1 @15:32:18.56 ARV.dial :air1 @15:32:18.90 Positioning Slug by 0.324ml @15:32:18.95 AS.seek 0.326ml @15:32:35.86 CBV.dial between CSV and waste2b @15:32:36.19 Pumping wcr puck from tube 2 up to 55psi... @15:32:36.23 CBV.dial SSV @15:32:37.66 CBV.dial between CSV and waste2b @15:32:38.59 CSV.dial between air and CTV @15:32:38.98 CS.seek 6.563ml @15:32:47.62 CTV.dial CSV,avoiding => air @15:32:47.64 CSV.dial CTV @15:32:48.40 CS.reconfigure CSpressurize @15:32:48.43 CS.seek 0.000ml @15:33:05.57 CSV.dial :air @15:33:06.08 CS.reconfigure CSfast @15:33:06.11 CS.seek 10.000ml @15:33:29.39 CSV.dial between air and CTV @15:33:30.38 CS.seek 2.139ml @15:33:48.96 CTV.dial CSV,avoiding => air @15:33:48.97 CSV.dial CTV @15:33:49.35 CS.reconfigure CSpressurize @15:33:49.38 CS.seek 0.000ml @15:33:50.50 CBV.dial :waste2b @15:33:50.82 CS.reconfigure CSconfig @15:33:50.85 CS.seek empty @15:33:56.29 delay 1:00 @15:34:56.30 CTV.dial :air,avoiding => intake @15:34:57.58 Fix #1 of 2 (with rnal) for 02:00.00 @15:34:57.59 CS Pulling 2ml of rnal CSV.dial CSR @15:34:58.02 CSR.select :rnal @15:34:58.12 CS.seek 2.000ml @15:35:04.10 delay 5 seconds @15:35:09.11 CSR.select :air @15:35:09.20 CS.reconfigure CSfast @15:35:09.24 CS.seek 10.000ml @15:35:28.10 CBV.dial between CSV and waste2b @15:35:28.44 Pumping wcr puck from tube 2 up to 55psi... @15:35:28.48 CBV.dial SSV @15:35:29.90 CBV.dial between CSV and waste2b @15:35:30.84 CSV.dial between air and CTV @15:35:32.10 CS.seek 6.497ml @15:35:40.83 CTV.dial CSV,avoiding => air @15:35:40.85 CSV.dial CTV @15:35:42.20 CS.reconfigure CSpressurize @15:35:42.23 CS.seek 0.000ml @15:35:58.59 delay 2:00 @15:37:49.57 delay 5 seconds @15:37:54.58 ATV.dial ARV @15:37:55.10 ASV.dial :PCRlock @15:37:55.87 delay 5 seconds @15:37:58.60 CBV.dial :waste2b @15:37:58.93 CS.reconfigure CSconfig @15:37:58.96 CS.seek empty @15:38:00.25 delay 1:00 @15:38:00.87 Running HF183em ProfileEM60C2 thermocycling @15:38:00.88 PCR.program ProfileEM60C2 @15:38:00.99 ProfileEM60C2 should complete in < 2:11:38 @15:38:01.00 PCR.run 2:49:32.5 @15:39:00.27 CTV.dial :air,avoiding => intake @15:39:01.55 Fix #2 of 2 (with rnal) for 02:00.00 @15:39:01.56 CS Pulling 2ml of rnal CSV.dial CSR @15:39:02.08 CSR.select :rnal @15:39:02.18 CS.seek 2.000ml @15:39:08.10 delay 5 seconds @15:39:13.11 CSR.select :air @15:39:13.20 CS.reconfigure CSfast @15:39:13.24 CS.seek 10.000ml @15:39:32.22 CBV.dial between CSV and waste2b @15:39:32.54 Pumping wcr puck from tube 2 up to 55psi... @15:39:32.59 CBV.dial SSV @15:39:34.01 CBV.dial between CSV and waste2b @15:39:34.94 CSV.dial between air and CTV @15:39:35.59 CS.seek 6.475ml @15:39:44.45 CTV.dial CSV,avoiding => air @15:39:44.47 CSV.dial CTV @15:39:45.76 CS.reconfigure CSpressurize @15:39:45.79 CS.seek 0.000ml @15:40:02.15 delay 2:00 @15:42:02.16 CBV.dial :waste2b @15:42:02.48 CS.reconfigure CSconfig @15:42:02.51 CS.seek empty @15:42:03.65 delay 1:00 @15:43:03.66 CTV.dial :air,avoiding => intake @15:43:04.97 Storing wcr puck in Collection Clamp to tube 1 @15:43:05.10 SC.reconfigure SCtight @15:43:05.14 SC.seek 1 @15:43:07.44 SC.reconfigure SCloose @15:43:07.62 Elbow.reconfigure TightElbow @15:43:07.65 Elbow.seek 1 @15:43:09.21 Forearm.reconfigure TightForearm @15:43:09.25 Forearm.seek 1 + 800 counts @15:43:10.26 SE.reconfigure SEpick @15:43:10.31 SE.seek beamBreak @15:43:12.85 SE.configure SEconfig @15:43:12.97 Elbow.reconfigure LimpElbow @15:43:13.00 Forearm.reconfigure LooseForearm @15:43:13.04 Forearm.seek 1 @15:43:13.25 Elbow.hold @15:43:13.31 Elbow.reconfigure TightElbow @15:43:13.39 SE.seek 4.04 Pucks below Top Plate @15:43:13.73 Forearm.coast @15:43:13.77 Forearm.seek 1 + 316 counts @15:43:14.00 Forearm.reconfigure TightForearm @15:43:14.03 Forearm.seek 1 + 800 counts @15:43:14.21 SE.seek 5.04 Pucks below Top Plate @15:43:15.38 Forearm.reconfigure LooseForearm @15:43:15.42 Forearm.seek retract @15:43:16.37 Elbow.seek CC @15:43:17.91 Elbow.reconfigure LooseElbow @15:43:17.99 Forearm.seek CC @15:43:18.94 CC.seek sealed @15:43:19.87 Hand.grip @15:43:20.58 CC.seek open @15:43:23.94 Forearm.seek retract @15:43:24.90 Elbow.reconfigure TightElbow @15:43:24.93 Elbow.seek 1 @15:43:26.55 Elbow.reconfigure LimpElbow @15:43:26.59 Forearm.seek 1 @15:43:27.60 Elbow.hold @15:43:27.65 Elbow.reconfigure TightElbow @15:43:27.69 Hand.release @15:43:28.54 Forearm.coast @15:43:28.58 Forearm.seek 1 + 336 counts @15:43:28.81 Forearm.reconfigure TightForearm @15:43:28.84 Forearm.seek 1 + 800 counts @15:43:29.19 SE.seek down @15:43:30.29 Forearm.reconfigure LooseForearm @15:43:30.33 Forearm.seek retract @15:43:31.42 SE.coast @15:43:31.45 SC.coast @15:43:31.48 Starting finalPurge @15:43:31.49 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:43:31.64 Elbow.seek FlushPuck::Garage @15:43:33.42 Elbow.reconfigure LooseElbow @15:43:33.50 Forearm.seek garage @15:43:34.53 Hand.grip @15:43:35.30 Forearm.seek retract @15:43:36.40 Elbow.reconfigure TightElbow @15:43:36.44 Elbow.seek CC @15:43:39.29 Elbow.reconfigure LooseElbow @15:43:39.37 Forearm.reconfigure TightForearm @15:43:39.40 Forearm.seek CC @15:43:40.52 CC.seek closed @15:43:44.33 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 2.02 @15:43:44.38 Hand.release @15:43:45.12 Forearm.reconfigure LooseForearm @15:43:45.15 Forearm.seek retract @15:43:46.10 Flushing the Collection Valve::Manifold @15:43:46.20 CS Pulling 5ml of flush CSV.dial CSR @15:43:46.63 CSR.select :flush @15:43:46.73 CS.seek 5.000ml @15:44:00.24 delay 5 seconds @15:44:05.25 CSR.select :air @15:44:05.39 CS.reconfigure CSfast @15:44:05.42 CS.seek 7.500ml @15:44:11.96 CSV.dial :waste @15:44:13.23 CS.reconfigure CSconfig @15:44:13.26 CS.seek 0.000ml @15:44:33.08 Flushing lower manifold's waste1 port @15:44:33.17 CSV.dial :flush @15:44:33.61 CS.reconfigure CSfast @15:44:33.64 CS.seek 5.000ml @15:44:45.77 delay 5 seconds @15:44:50.78 CSV.dial :air @15:44:51.27 CS.seek 7.000ml @15:44:56.74 CSV.dial CTV @15:44:56.76
CTV.dial CSV @15:44:58.08 CBV.dial :waste1 @15:44:58.84 CS.seek 0.000ml @15:45:15.46 Flushing lower manifold's waste2b port @15:45:15.56 CSV.dial :flush @15:45:16.37 CS.seek 5.000ml @15:45:28.46 delay 5 seconds @15:45:33.47 CSV.dial :air @15:45:34.04 CS.seek 7.000ml @15:45:39.45 CSV.dial CTV @15:45:39.47
CTV.dial CSV @15:45:39.92 CBV.dial :waste2b @15:45:40.68 CS.seek 0.000ml @15:45:57.11 Flushing upper manifold @15:45:57.20 CSV.dial :flush @15:45:58.01 CS.seek 5.000ml @15:46:10.11 delay 5 seconds @15:46:15.12 CSV.dial :air @15:46:15.69 CS.seek 7.000ml @15:46:21.11 CSV.dial CBV @15:46:21.13
CBV.dial CSV @15:46:22.26 CTV.dial :air @15:46:23.56 CS.seek 0.000ml @15:46:40.11 delay 7 seconds @15:46:47.12 CS.seek 3.000ml @15:46:54.73 CSV.dial :waste @15:46:55.24 CS.seek 0.000ml @15:47:02.92 CSV.dial :flush @15:47:03.35 CS.seek 3.000ml @15:47:10.95 delay 5 seconds @15:47:15.96 CSV.dial :air @15:47:16.45 CS.seek 5.000ml @15:47:21.92 CSV.dial :air @15:47:21.94 CS.seek 0.000ml @15:47:33.95 delay 7 seconds @15:47:40.96 CS.seek 3.000ml @15:47:48.54 CSV.dial :waste @15:47:49.35 CS.seek 0.000ml @15:47:57.01 Disinfecting external Intake with mfbkill @15:47:57.11 CSV.dial CSR @15:47:58.35 CSR.select :mfbkill @15:47:58.45 CS.seek 8.000ml @15:48:17.42 delay 5 seconds @15:48:22.43 CSR.select :air @15:48:22.52 CS.seek 10.000ml @15:48:27.88 CSV.dial CBV @15:48:27.90
CBV.dial CSV @15:48:28.97 CTV.dial :intake @15:48:29.70 Intake.select :open @15:48:29.80 CS.seek 2.000ml @15:48:48.88 Intake.select :close @15:48:48.97 CTV.dial :air @15:48:49.72 CS.reconfigure CSconfig @15:48:49.75 CS.seek 0.000ml @15:48:55.66 Flushing lower manifold's waste1 port @15:48:55.67 CS Pulling 4ml of mfbkill @15:48:55.68 CSV.dial CSR @15:48:56.73 CSR.select :mfbkill @15:48:56.83 CS.seek 4.000ml @15:49:07.79 delay 5 seconds @15:49:12.80 CSR.select :air @15:49:12.89 CS.reconfigure CSfast @15:49:12.93 CS.seek 10.000ml @15:49:27.27 CSV.dial CTV @15:49:27.29
CTV.dial CSV @15:49:28.64 CBV.dial :waste1 @15:49:29.63 CS.seek 0.000ml @15:49:52.97 Flushing lower manifold's waste2b port @15:49:52.98 CS Pulling 4ml of mfbkill @15:49:52.99 CSV.dial CSR @15:49:53.90 CSR.select :mfbkill @15:49:54.00 CS.reconfigure CSconfig @15:49:54.04 CS.seek 4.000ml @15:50:05.00 delay 5 seconds @15:50:10.01 CSR.select :air @15:50:10.10 CS.reconfigure CSfast @15:50:10.14 CS.seek 10.000ml @15:50:24.42 CSV.dial CTV @15:50:24.44
CTV.dial CSV @15:50:24.95 CBV.dial :waste2b @15:50:25.69 CS.seek 0.000ml @15:50:48.82 Killing upper manifold @15:50:48.83 CS Pulling 4ml of mfbkill @15:50:48.84 CSV.dial CSR @15:50:49.35 CSR.select :mfbkill @15:50:49.45 CS.reconfigure CSconfig @15:50:49.49 CS.seek 4.000ml @15:51:00.50 delay 5 seconds @15:51:05.51 CSR.select :air @15:51:05.60 CS.reconfigure CSfast @15:51:05.64 CS.seek 10.000ml @15:51:19.81 CSV.dial CBV @15:51:19.83
CBV.dial CSV @15:51:21.26 CTV.dial :air @15:51:22.53 CS.seek 0.000ml @15:51:45.63 delay 7 seconds @15:51:52.64 CS.seek 3.000ml @15:52:00.19 CSV.dial :waste @15:52:00.84 CS.seek 0.000ml @15:52:08.44 Flushing upper manifold @15:52:08.53 CSV.dial :flush @15:52:09.06 CS.seek 5.000ml @15:52:21.15 delay 5 seconds @15:52:26.16 CSV.dial :air @15:52:26.63 CS.seek 7.000ml @15:52:31.97 CSV.dial CBV @15:52:31.99
CBV.dial CSV @15:52:33.07 CTV.dial :air @15:52:33.08 CS.seek 0.000ml @15:52:49.47 delay 7 seconds @15:52:56.48 CS.seek 3.000ml @15:53:04.06 CSV.dial :waste @15:53:04.57 CS.seek 0.000ml @15:53:12.58 CSV.dial :flush @15:53:13.02 CS.seek 3.000ml @15:53:20.62 delay 5 seconds @15:53:25.63 CSV.dial :air @15:53:26.12 CS.seek 5.000ml @15:53:31.53 CSV.dial :air @15:53:31.55 CS.seek 0.000ml @15:53:43.46 delay 7 seconds @15:53:50.47 CS.seek 3.000ml @15:53:58.15 CSV.dial :waste @15:53:58.96 CS.seek 0.000ml @15:54:06.56 Flushing lower manifold's waste1 port @15:54:06.66 CSV.dial :flush @15:54:07.16 CS.seek 5.000ml @15:54:19.24 delay 5 seconds @15:54:24.25 CSV.dial :air @15:54:24.71 CS.seek 7.000ml @15:54:30.06 CSV.dial CTV @15:54:30.08
CTV.dial CSV @15:54:31.40 CBV.dial :waste1 @15:54:31.85 CS.seek 0.000ml @15:54:48.31 Flushing lower manifold's waste2b port @15:54:48.40 CSV.dial :flush @15:54:49.19 CS.seek 5.000ml @15:55:01.27 delay 5 seconds @15:55:06.28 CSV.dial :air @15:55:06.82 CS.seek 7.000ml @15:55:12.24 CSV.dial CTV @15:55:12.82
CTV.dial CSV @15:55:13.48 CBV.dial :waste2b @15:55:14.22 CS.seek 0.000ml @15:55:30.68 Disinfecting external Intake with kill @15:55:30.77 CSV.dial CSR @15:55:31.29 CSR.select :kill @15:55:31.39 CS.seek 8.000ml @15:55:50.31 delay 5 seconds @15:55:55.32 CSR.select :air @15:55:55.41 CS.seek 10.000ml @15:56:00.78 CSV.dial CBV @15:56:00.80
CBV.dial CSV @15:56:01.82 CTV.dial :intake @15:56:02.54 Intake.select :open @15:56:02.65 CS.seek 2.000ml @15:56:21.56 Intake.select :close @15:56:21.65 CTV.dial :air @15:56:22.38 CS.reconfigure CSconfig @15:56:22.41 CS.seek 0.000ml @15:56:28.31 CSV.dial CSR @15:56:29.36 CSR.select :flush @15:56:29.46 CS.seek 5.000ml @15:56:42.97 delay 5 seconds @15:56:47.98 CSR.select :air @15:56:48.07 CS.seek 10.000ml @15:57:01.62 CSV.dial CBV @15:57:02.68 CS.seek 0.000ml @15:57:28.78 Garaging FlushPuck in Collection Clamp @15:57:28.84 Elbow.reconfigure TightElbow @15:57:28.87 Elbow.seek CC @15:57:29.06 Elbow.reconfigure LooseElbow @15:57:29.14 Forearm.seek CC @15:57:30.07 CC.seek sealed @15:57:30.99 Hand.grip @15:57:31.68 CC.seek open @15:57:35.00 Forearm.seek retract @15:57:35.94 Elbow.reconfigure TightElbow @15:57:35.98 Elbow.seek garage @15:57:38.77 Elbow.reconfigure LooseElbow @15:57:38.85 Forearm.reconfigure TightForearm @15:57:38.88 Forearm.seek garage @15:57:40.03 Hand.release @15:57:40.84 Forearm.reconfigure LooseForearm @15:57:40.87 Forearm.seek retract @15:57:41.89 Completed {wqPCR} begun 12:58:42 @15:57:41.91 deferred until MFBPCR finish @15:57:41.93
Waiting for MFBPCR til 8AM 2/15/14 @15:57:41.95 Sleeping until 08:00PST15-Feb-14 @15:57:41.96 GoodNews.email "Then, in tube 2: wqPCR\n/home/esp/esp2local/mission/14feb14sccwrp.rb:58",Subject => "Waiting until 08:00PST15-Feb-14" @16:04:13.25 Session Begun @16:04:32.60 -> showlog 50 @16:06:46.40 Session Ended @17:34:22.89 PCR.off @17:34:23.95 PCR for HF183em completed -- closed /var/log/esp/HF183em14feb1415h700ml.pcr @17:34:24.00 Cleaning PCR @17:34:24.01 AFV.select :carrierSolution @17:34:24.10 delay 2 seconds @17:34:26.11 AS.reconfigure ASconfig @17:34:26.15 AS.seek 0.500ml @17:34:36.58 delay 1 second @17:34:37.59 AFV.select :holdingCoil @17:34:37.68 ASV.dial :waste1 @17:34:38.36 ATV.dial :air3 @17:34:39.31 AS.reconfigure ASslow1 @17:34:39.35 AS.seek 0.510ml @17:34:41.65 delay 2 seconds @17:34:43.66 ATV.dial :bleach @17:34:44.03 AS.seek 0.810ml @17:35:45.48 delay 3 seconds @17:35:48.49 ATV.dial :air3 @17:35:48.92 delay 3 seconds @17:35:51.98 AS.seek 0.835ml @17:35:57.32 delay 3 seconds @17:36:00.33 ATV.dial :bleach @17:36:00.70 AS.seek 0.820ml @17:36:04.03 delay 3 seconds @17:36:07.04 ARV.dial :waste @17:36:07.54 delay 3 seconds @17:36:10.55 ATV.dial :ARV @17:36:11.37 AS.reconfigure ASconfig @17:36:11.40 AS.seek 0.470ml @17:36:32.10 AFV.select :carrierSolution @17:36:32.19 delay 2 seconds @17:36:34.20 AS.seek 1.000ml @17:37:05.38 delay 1 second @17:37:06.39 AFV.select :holdingCoil @17:37:06.48 delay 1 second @17:37:07.49 AS.reconfigure ASfast @17:37:07.52 AS.seek 0.000ml @17:37:35.24 AFV.select :carrierSolution @17:37:35.34 delay 2 seconds @17:37:37.35 AS.reconfigure ASconfig @17:37:37.38 AS.seek 1.000ml @17:38:36.01 delay 1 second @17:38:37.02 AFV.select :holdingCoil @17:38:37.11 delay 1 second @17:38:38.12 AS.reconfigure ASfast @17:38:38.15 AS.seek 0.000ml @17:39:05.89 AFV.select :carrierSolution @17:39:05.99 delay 2 seconds @17:39:08.00 AS.reconfigure ASconfig @17:39:08.03 AS.seek 1.000ml @17:40:06.62 delay 1 second @17:40:07.63 AFV.select :holdingCoil @17:40:07.72 delay 1 second @17:40:08.73 AS.reconfigure ASfast @17:40:08.76 AS.seek 0.000ml @17:40:36.48 ARV.dial between carrier and air1 @17:40:36.89 ATV.dial :air3 @17:40:37.83 AS.reconfigure ASslow1 @17:40:37.86 AS.seek 0.010ml @17:40:40.23 delay 2 seconds @17:40:42.24 ATV.dial :bleach @17:40:42.62 AS.seek 0.810ml @17:43:26.02 delay 3 seconds @17:43:29.03 ATV.dial :air3 @17:43:29.36 delay 3 seconds @17:43:32.42 AS.seek 0.835ml @17:43:37.77 delay 3 seconds @17:43:40.78 ATV.dial :bleach @17:43:41.18 AS.seek 0.820ml @17:43:44.49 delay 3 seconds @17:43:47.50 ATV.dial PCR @17:43:47.83 AS.reconfigure ASconfig @17:43:47.86 AS.seek empty @17:44:35.99 PCR.program :bleachProfile @17:44:36.05 PCR program should complete in < 2:45 @17:44:36.06 PCR.run 10:00 @17:47:34.25 PCR.off @17:47:34.28 AFV.select :carrierSolution @17:47:34.37 delay 2 seconds @17:47:36.38 AS.seek 1.000ml @17:48:35.06 delay 1 second @17:48:36.07 AFV.select :holdingCoil @17:48:36.16 delay 1 second @17:48:37.17 AS.reconfigure ASfast @17:48:37.20 AS.seek 0.000ml @17:49:04.93 delay 3 seconds @17:49:07.94 AFV.select :carrierSolution @17:49:08.03 delay 2 seconds @17:49:10.04 AS.reconfigure ASconfig @17:49:10.07 AS.seek 1.000ml @17:50:08.66 delay 1 second @17:50:09.67 AFV.select :holdingCoil @17:50:09.76 delay 1 second @17:50:10.77 AS.reconfigure ASfast @17:50:10.80 AS.seek 0.000ml @17:50:38.54 delay 3 seconds @17:50:41.55 AFV.select :carrierSolution @17:50:41.64 delay 2 seconds @17:50:43.65 AS.reconfigure ASconfig @17:50:43.68 AS.seek 1.000ml @17:51:42.29 delay 1 second @17:51:43.30 AFV.select :holdingCoil @17:51:43.39 delay 1 second @17:51:44.40 AS.reconfigure ASfast @17:51:44.43 AS.seek 0.000ml @17:52:12.16 delay 3 seconds @17:52:15.17 AFV.select :carrierSolution @17:52:15.26 delay 2 seconds @17:52:17.27 AS.reconfigure ASconfig @17:52:17.30 AS.seek 1.000ml @17:53:15.91 delay 1 second @17:53:16.92 AFV.select :holdingCoil @17:53:17.01 delay 1 second @17:53:18.02 AS.reconfigure ASfast @17:53:18.05 AS.seek 0.000ml @17:53:45.78 delay 3 seconds @17:53:48.79 AFV.select :carrierSolution @17:53:48.88 delay 2 seconds @17:53:50.89 AS.reconfigure ASconfig @17:53:50.92 AS.seek 1.000ml @17:54:49.53 delay 1 second @17:54:50.54 AFV.select :holdingCoil @17:54:50.63 delay 1 second @17:54:51.64 AS.reconfigure ASnorm @17:54:51.67 AS.seek 0.000ml @17:55:43.13 Setting up PCR for ENTap -- writing /var/log/esp/ENTap14feb1417h700ml.pcr @17:55:43.14 Building PCR Slug from ASV with MM1 mix, PP1 primer @17:55:43.15 Priming with carrier @17:55:43.16 ATV.dial ARV @17:55:43.68 ARV.dial :carrier @17:55:44.49 AS.seek 0.500ml @17:56:10.38 AFV.select :carrierSolution @17:56:10.49 delay 2 seconds @17:56:12.50 AS.reconfigure ASconfig @17:56:12.54 AS.seek 1.000ml @17:56:41.99 delay 1 second @17:56:43.00 AFV.select :holdingCoil @17:56:43.10 delay 1 second @17:56:44.11 ATV.dial :waste @17:56:45.37 AS.reconfigure ASnorm @17:56:45.40 AS.seek empty @17:57:36.89 AFV.select :carrierSolution @17:57:37.00 delay 2 seconds @17:57:39.01 AS.reconfigure ASconfig @17:57:39.04 AS.seek 0.500ml @17:58:08.53 delay 1 second @17:58:09.54 AFV.select :holdingCoil @17:58:09.64 Building Slug @17:58:09.65 ATV.dial :ARV @17:58:10.89 delay 2 seconds @17:58:12.90 ARV.dial :air1 @17:58:13.22 delay 2 seconds @17:58:15.28 AS.reconfigure ASslow2 @17:58:15.31 AS.seek 0.506ml @17:58:21.91 delay 2 seconds @17:58:23.92 ARV.dial :MM1 @17:58:24.42 delay 2 seconds @17:58:26.43 Adding MM1 @17:58:26.49 AS.seek 0.524ml @17:58:45.27 delay 25 seconds @17:59:10.28 ARV.dial :PP1 @17:59:10.62 Adding PP1 @17:59:10.63 delay 2 seconds @17:59:12.68 AS.seek 0.530ml @17:59:19.17 delay 10 seconds @17:59:29.18 ARV.dial :air1 @17:59:29.49 adding ARV air @17:59:29.50 delay 2 seconds @17:59:31.55 AS.seek 0.536ml @17:59:37.80 delay 5 seconds @17:59:42.81 ARV.dial :carrier @17:59:43.12 delay 7 seconds @17:59:50.18 AS.seek 0.594ml @18:00:49.53 delay 5 seconds @18:00:54.54 ATV.dial :ASV @18:00:54.87 delay 2 seconds @18:00:56.93 AS.seek 0.600ml @18:01:03.22 delay 4 seconds @18:01:07.23 ATV.dial ARV @18:01:07.56 delay 2 seconds @18:01:09.57 ARV.dial :carrier @18:01:09.58 delay 2 seconds @18:01:11.63 AS.seek 0.650ml @18:02:02.84 delay 4 seconds @18:02:06.85 ATV.dial PCR @18:02:07.35 ASV.dial :waste1 @18:02:07.36 ARV.dial :air1 @18:02:07.71 Positioning Slug by 0.324ml @18:02:07.76 AS.seek 0.326ml @18:07:38.25 delay 5 seconds @18:07:43.26 ATV.dial ARV @18:07:43.86 ASV.dial :PCRlock @18:07:44.32 delay 5 seconds @18:07:49.34 Running ENTap Profile60C thermocycling @18:07:49.35 PCR.program Profile60C @18:07:49.45 Profile60C should complete in < 1:39:08 @18:07:49.46 PCR.run 2:08:55 @19:30:23.38 PCR.off @19:30:23.90 PCR for ENTap completed -- closed /var/log/esp/ENTap14feb1417h700ml.pcr @19:30:23.95 Cleaning PCR @19:30:23.96 AFV.select :carrierSolution @19:30:24.05 delay 2 seconds @19:30:26.06 AS.reconfigure ASconfig @19:30:26.09 AS.seek 0.500ml @19:30:36.53 delay 1 second @19:30:37.54 AFV.select :holdingCoil @19:30:37.63 ASV.dial :waste1 @19:30:38.06 ATV.dial :air3 @19:30:39.00 AS.reconfigure ASslow1 @19:30:39.03 AS.seek 0.510ml @19:30:41.23 delay 2 seconds @19:30:43.24 ATV.dial :bleach @19:30:43.61 AS.seek 0.810ml @19:31:45.04 delay 3 seconds @19:31:48.05 ATV.dial :air3 @19:31:48.39 delay 3 seconds @19:31:51.45 AS.seek 0.835ml @19:31:56.81 delay 3 seconds @19:31:59.82 ATV.dial :bleach @19:32:00.22 AS.seek 0.820ml @19:32:03.53 delay 3 seconds @19:32:06.54 ARV.dial :waste @19:32:07.06 delay 3 seconds @19:32:10.07 ATV.dial :ARV @19:32:10.80 AS.reconfigure ASconfig @19:32:10.84 AS.seek 0.470ml @19:32:31.62 AFV.select :carrierSolution @19:32:31.71 delay 2 seconds @19:32:33.72 AS.seek 1.000ml @19:33:04.93 delay 1 second @19:33:05.94 AFV.select :holdingCoil @19:33:06.03 delay 1 second @19:33:07.04 AS.reconfigure ASfast @19:33:07.07 AS.seek 0.000ml @19:33:34.79 AFV.select :carrierSolution @19:33:34.89 delay 2 seconds @19:33:36.90 AS.reconfigure ASconfig @19:33:36.93 AS.seek 1.000ml @19:34:35.52 delay 1 second @19:34:36.53 AFV.select :holdingCoil @19:34:36.62 delay 1 second @19:34:37.63 AS.reconfigure ASfast @19:34:37.66 AS.seek 0.000ml @19:35:05.40 AFV.select :carrierSolution @19:35:05.50 delay 2 seconds @19:35:07.51 AS.reconfigure ASconfig @19:35:07.54 AS.seek 1.000ml @19:36:06.14 delay 1 second @19:36:07.15 AFV.select :holdingCoil @19:36:07.24 delay 1 second @19:36:08.25 AS.reconfigure ASfast @19:36:08.28 AS.seek 0.000ml @19:36:36.00 ARV.dial between carrier and air1 @19:36:36.43 ATV.dial :air3 @19:36:37.46 AS.reconfigure ASslow1 @19:36:37.49 AS.seek 0.010ml @19:36:39.86 delay 2 seconds @19:36:41.87 ATV.dial :bleach @19:36:42.35 AS.seek 0.810ml @19:39:25.82 delay 3 seconds @19:39:28.83 ATV.dial :air3 @19:39:29.16 delay 3 seconds @19:39:32.22 AS.seek 0.835ml @19:39:37.55 delay 3 seconds @19:39:40.56 ATV.dial :bleach @19:39:40.95 AS.seek 0.820ml @19:39:44.26 delay 3 seconds @19:39:47.27 ATV.dial PCR @19:39:47.60 AS.reconfigure ASconfig @19:39:47.63 AS.seek empty @19:40:35.69 PCR.program :bleachProfile @19:40:35.75 PCR program should complete in < 2:45 @19:40:35.76 PCR.run 10:00 @19:43:36.97 PCR.off @19:43:37.00 AFV.select :carrierSolution @19:43:37.09 delay 2 seconds @19:43:39.10 AS.seek 1.000ml @19:44:37.69 delay 1 second @19:44:38.70 AFV.select :holdingCoil @19:44:38.79 delay 1 second @19:44:39.80 AS.reconfigure ASfast @19:44:39.83 AS.seek 0.000ml @19:45:07.56 delay 3 seconds @19:45:10.57 AFV.select :carrierSolution @19:45:10.66 delay 2 seconds @19:45:12.67 AS.reconfigure ASconfig @19:45:13.25 AS.seek 1.000ml @19:46:11.84 delay 1 second @19:46:12.85 AFV.select :holdingCoil @19:46:12.94 delay 1 second @19:46:13.95 AS.reconfigure ASfast @19:46:13.98 AS.seek 0.000ml @19:46:41.71 delay 3 seconds @19:46:44.72 AFV.select :carrierSolution @19:46:44.81 delay 2 seconds @19:46:46.82 AS.reconfigure ASconfig @19:46:46.85 AS.seek 1.000ml @19:47:45.46 delay 1 second @19:47:46.47 AFV.select :holdingCoil @19:47:46.56 delay 1 second @19:47:47.57 AS.reconfigure ASfast @19:47:47.60 AS.seek 0.000ml @19:48:15.33 delay 3 seconds @19:48:18.34 AFV.select :carrierSolution @19:48:18.43 delay 2 seconds @19:48:20.44 AS.reconfigure ASconfig @19:48:20.47 AS.seek 1.000ml @19:49:19.08 delay 1 second @19:49:20.09 AFV.select :holdingCoil @19:49:20.18 delay 1 second @19:49:21.19 AS.reconfigure ASfast @19:49:21.22 AS.seek 0.000ml @19:49:48.94 delay 3 seconds @19:49:51.95 AFV.select :carrierSolution @19:49:52.04 delay 2 seconds @19:49:54.05 AS.reconfigure ASconfig @19:49:54.08 AS.seek 1.000ml @19:50:52.64 delay 1 second @19:50:53.65 AFV.select :holdingCoil @19:50:53.74 delay 1 second @19:50:54.75 AS.reconfigure ASnorm @19:50:54.78 AS.seek 0.000ml @19:51:46.26 Setting up PCR for ENTem -- writing /var/log/esp/ENTem14feb1419h700ml.pcr @19:51:46.27 Building PCR Slug from ASV with MM2 mix, PP3 primer @19:51:46.28 Priming with carrier ATV.dial ARV @19:51:46.80 ARV.dial :carrier @19:51:47.10 AS.seek 0.500ml @19:52:12.98 AFV.select :carrierSolution @19:52:13.09 delay 2 seconds @19:52:15.10 AS.reconfigure ASconfig @19:52:15.14 AS.seek 1.000ml @19:52:44.59 delay 1 second @19:52:45.60 AFV.select :holdingCoil @19:52:45.70 delay 1 second @19:52:46.71 ATV.dial :waste @19:52:48.06 AS.reconfigure ASnorm @19:52:48.09 AS.seek empty @19:53:39.54 AFV.select :carrierSolution @19:53:39.65 delay 2 seconds @19:53:41.66 AS.reconfigure ASconfig @19:53:41.69 AS.seek 0.500ml @19:54:11.17 delay 1 second @19:54:12.18 AFV.select :holdingCoil @19:54:12.27 Building Slug @19:54:12.28 ATV.dial :ARV @19:54:13.53 delay 2 seconds @19:54:15.54 ARV.dial :air1 @19:54:15.87 delay 2 seconds @19:54:17.93 AS.reconfigure ASslow2 @19:54:17.96 AS.seek 0.506ml @19:54:24.48 delay 2 seconds @19:54:26.49 ARV.dial :MM2 @19:54:27.82 delay 2 seconds @19:54:29.83 Adding MM2 @19:54:29.89 AS.seek 0.524ml @19:54:48.55 delay 25 seconds @19:55:13.56 ARV.dial :PP3 @19:55:13.88 Adding PP3 @19:55:13.89 delay 2 seconds @19:55:15.95 AS.seek 0.530ml @19:55:22.25 delay 10 seconds @19:55:32.26 ARV.dial :air2 @19:55:32.58 adding ARV air @19:55:32.59 delay 2 seconds @19:55:34.65 AS.seek 0.536ml @19:55:36.87 Session Begun @19:55:41.07 delay 5 seconds @19:55:46.08 ARV.dial :carrier @19:55:47.13 delay 7 seconds @19:55:54.19 AS.seek 0.594ml @19:56:09.61 -> exit @19:56:09.62 Session Ended @19:56:53.53 delay 5 seconds @19:56:58.54 ATV.dial :ASV @19:56:58.88 delay 2 seconds @19:57:00.93 AS.seek 0.600ml @19:57:07.31 delay 4 seconds @19:57:11.32 ATV.dial ARV @19:57:11.64 delay 2 seconds @19:57:13.65 ARV.dial :carrier @19:57:13.66 delay 2 seconds @19:57:15.71 AS.seek 0.650ml @19:58:07.23 delay 4 seconds @19:58:11.24 ATV.dial PCR @19:58:11.73 ASV.dial :waste1 @19:58:11.74 ARV.dial :air1 @19:58:12.09 Positioning Slug by 0.324ml @19:58:12.15 AS.seek 0.326ml @20:03:42.64 delay 5 seconds @20:03:47.65 ATV.dial ARV @20:03:48.16 ASV.dial :PCRlock @20:03:48.64 delay 5 seconds @20:03:53.65 Running ENTem ProfileEM60C thermocycling @20:03:53.66 PCR.program ProfileEM60C @20:03:53.77 ProfileEM60C should complete in < 2:11:38 @20:03:53.78 PCR.run 2:49:32.5 @21:58:24.55 PCR.off @21:58:25.09 PCR for ENTem completed -- closed /var/log/esp/ENTem14feb1419h700ml.pcr @21:58:25.14 Cleaning PCR @21:58:25.15 AFV.select :carrierSolution @21:58:25.24 delay 2 seconds @21:58:27.25 AS.reconfigure ASconfig @21:58:27.28 AS.seek 0.500ml @21:58:37.75 delay 1 second @21:58:38.76 AFV.select :holdingCoil @21:58:38.85 ASV.dial :waste1 @21:58:39.33 ATV.dial :air3 @21:58:40.36 AS.reconfigure ASslow1 @21:58:40.39 AS.seek 0.510ml @21:58:42.59 delay 2 seconds @21:58:44.60 ATV.dial :bleach @21:58:44.97 AS.seek 0.810ml @21:59:46.44 delay 3 seconds @21:59:49.45 ATV.dial :air3 @21:59:49.78 delay 3 seconds @21:59:52.84 AS.seek 0.835ml @21:59:58.18 delay 3 seconds @22:00:01.19 ATV.dial :bleach @22:00:01.56 AS.seek 0.820ml @22:00:04.86 delay 3 seconds @22:00:07.87 ARV.dial :waste @22:00:08.37 delay 3 seconds @22:00:11.38 ATV.dial :ARV @22:00:12.21 AS.reconfigure ASconfig @22:00:12.25 AS.seek 0.470ml @22:00:32.93 AFV.select :carrierSolution @22:00:33.02 delay 2 seconds @22:00:35.03 AS.seek 1.000ml @22:01:06.25 delay 1 second @22:01:07.26 AFV.select :holdingCoil @22:01:07.35 delay 1 second @22:01:08.36 AS.reconfigure ASfast @22:01:08.39 AS.seek 0.000ml @22:01:36.13 AFV.select :carrierSolution @22:01:36.23 delay 2 seconds @22:01:38.24 AS.reconfigure ASconfig @22:01:38.27 AS.seek 1.000ml @22:02:36.87 delay 1 second @22:02:37.88 AFV.select :holdingCoil @22:02:37.97 delay 1 second @22:02:38.98 AS.reconfigure ASfast @22:02:39.02 AS.seek 0.000ml @22:03:06.71 AFV.select :carrierSolution @22:03:06.81 delay 2 seconds @22:03:08.82 AS.reconfigure ASconfig @22:03:08.85 AS.seek 1.000ml @22:04:07.44 delay 1 second @22:04:08.45 AFV.select :holdingCoil @22:04:08.54 delay 1 second @22:04:09.55 AS.reconfigure ASfast @22:04:09.58 AS.seek 0.000ml @22:04:37.30 ARV.dial between carrier and air1 @22:04:37.82 ATV.dial :air3 @22:04:38.76 AS.reconfigure ASslow1 @22:04:38.79 AS.seek 0.010ml @22:04:41.14 delay 2 seconds @22:04:43.15 ATV.dial :bleach @22:04:44.19 AS.seek 0.810ml @22:07:27.53 delay 3 seconds @22:07:30.54 ATV.dial :air3 @22:07:30.87 delay 3 seconds @22:07:33.93 AS.seek 0.835ml @22:07:39.33 delay 3 seconds @22:07:42.34 ATV.dial :bleach @22:07:42.74 AS.seek 0.820ml @22:07:46.08 delay 3 seconds @22:07:49.09 ATV.dial PCR @22:07:49.41 AS.reconfigure ASconfig @22:07:49.44 AS.seek empty @22:08:37.54 PCR.program :bleachProfile @22:08:37.60 PCR program should complete in < 2:45 @22:08:37.61 PCR.run 10:00 @22:11:38.83 PCR.off @22:11:38.86 AFV.select :carrierSolution @22:11:38.95 delay 2 seconds @22:11:40.96 AS.seek 1.000ml @22:12:39.62 delay 1 second @22:12:40.63 AFV.select :holdingCoil @22:12:40.72 delay 1 second @22:12:41.73 AS.reconfigure ASfast @22:12:41.76 AS.seek 0.000ml @22:13:09.50 delay 3 seconds @22:13:12.51 AFV.select :carrierSolution @22:13:12.60 delay 2 seconds @22:13:14.61 AS.reconfigure ASconfig @22:13:14.65 AS.seek 1.000ml @22:14:13.23 delay 1 second @22:14:14.24 AFV.select :holdingCoil @22:14:14.33 delay 1 second @22:14:15.34 AS.reconfigure ASfast @22:14:15.37 AS.seek 0.000ml @22:14:43.09 delay 3 seconds @22:14:46.10 AFV.select :carrierSolution @22:14:46.19 delay 2 seconds @22:14:48.20 AS.reconfigure ASconfig @22:14:48.23 AS.seek 1.000ml @22:15:46.84 delay 1 second @22:15:47.85 AFV.select :holdingCoil @22:15:47.94 delay 1 second @22:15:48.95 AS.reconfigure ASfast @22:15:48.98 AS.seek 0.000ml @22:16:16.71 delay 3 seconds @22:16:19.72 AFV.select :carrierSolution @22:16:19.81 delay 2 seconds @22:16:21.82 AS.reconfigure ASconfig @22:16:21.85 AS.seek 1.000ml @22:17:20.46 delay 1 second @22:17:21.47 AFV.select :holdingCoil @22:17:21.56 delay 1 second @22:17:22.57 AS.reconfigure ASfast @22:17:22.60 AS.seek 0.000ml @22:17:50.33 delay 3 seconds @22:17:53.34 AFV.select :carrierSolution @22:17:53.43 delay 2 seconds @22:17:55.44 AS.reconfigure ASconfig @22:17:55.47 AS.seek 1.000ml @22:18:54.05 delay 1 second @22:18:55.06 AFV.select :holdingCoil @22:18:55.15 delay 1 second @22:18:56.16 AS.reconfigure ASnorm @22:18:56.19 AS.seek 0.000ml @22:19:47.64 Setting up PCR for IPCap -- writing /var/log/esp/IPCap14feb1422h700ml.pcr @22:19:47.65 Building PCR Slug from ASV with MM1 mix, PP2 primer @22:19:47.66 Priming with carrier ATV.dial ARV @22:19:48.17 ARV.dial :carrier @22:19:48.44 AS.seek 0.500ml @22:20:14.33 AFV.select :carrierSolution @22:20:14.44 delay 2 seconds @22:20:16.45 AS.reconfigure ASconfig @22:20:16.49 AS.seek 1.000ml @22:20:45.94 delay 1 second @22:20:46.95 AFV.select :holdingCoil @22:20:47.05 delay 1 second @22:20:48.06 ATV.dial :waste @22:20:49.34 AS.reconfigure ASnorm @22:20:49.37 AS.seek empty @22:21:40.84 AFV.select :carrierSolution @22:21:40.95 delay 2 seconds @22:21:42.96 AS.reconfigure ASconfig @22:21:42.99 AS.seek 0.500ml @22:22:12.50 delay 1 second @22:22:13.51 AFV.select :holdingCoil @22:22:13.61 Building Slug @22:22:13.62 ATV.dial :ARV @22:22:14.97 delay 2 seconds @22:22:16.98 ARV.dial :air1 @22:22:17.31 delay 2 seconds @22:22:19.37 AS.reconfigure ASslow2 @22:22:19.40 AS.seek 0.506ml @22:22:25.90 delay 2 seconds @22:22:27.91 ARV.dial :MM1 @22:22:28.40 delay 2 seconds @22:22:30.41 Adding MM1 @22:22:30.47 AS.seek 0.524ml @22:22:49.22 delay 25 seconds @22:23:14.23 ARV.dial :PP2 @22:23:14.64 Adding PP2 @22:23:14.65 delay 2 seconds @22:23:16.71 AS.seek 0.530ml @22:23:23.14 delay 10 seconds @22:23:33.15 ARV.dial :air2 @22:23:33.48 adding ARV air @22:23:33.49 delay 2 seconds @22:23:35.55 AS.seek 0.536ml @22:23:42.03 delay 5 seconds @22:23:47.04 ARV.dial :carrier @22:23:48.09 delay 7 seconds @22:23:55.15 AS.seek 0.594ml @22:24:54.71 delay 5 seconds @22:24:59.72 ATV.dial :ASV @22:25:00.04 delay 2 seconds @22:25:02.10 AS.seek 0.600ml @22:25:08.62 delay 4 seconds @22:25:12.63 ATV.dial ARV @22:25:12.96 delay 2 seconds @22:25:14.97 ARV.dial :carrier @22:25:14.98 delay 2 seconds @22:25:17.03 AS.seek 0.650ml @22:26:08.27 delay 4 seconds @22:26:12.81 ATV.dial PCR @22:26:13.32 ASV.dial :waste1 @22:26:13.33 ARV.dial :air1 @22:26:13.67 Positioning Slug by 0.324ml @22:26:13.72 AS.seek 0.326ml @22:31:44.41 delay 5 seconds @22:31:49.42 ATV.dial ARV @22:31:50.02 ASV.dial :PCRlock @22:31:50.51 delay 5 seconds @22:31:55.52 Running IPCap Profile59C thermocycling @22:31:55.53 PCR.program Profile59C @22:31:55.63 Profile59C should complete in < 1:39:08 @22:31:55.64 PCR.run 2:08:55 @22:55:01.74 Session Begun @22:55:12.00 -> showlog @22:56:08.56 -> exit Session Ended @23:53:55.09 PCR.off @23:53:55.62 PCR for IPCap completed -- closed /var/log/esp/IPCap14feb1422h700ml.pcr @23:53:55.67 Cleaning PCR @23:53:55.68 AFV.select :carrierSolution @23:53:55.77 delay 2 seconds @23:53:57.78 AS.reconfigure ASconfig @23:53:57.81 AS.seek 0.500ml @23:54:08.23 delay 1 second @23:54:09.24 AFV.select :holdingCoil @23:54:09.33 ASV.dial :waste1 @23:54:09.81 ATV.dial :air3 @23:54:10.75 AS.reconfigure ASslow1 @23:54:10.78 AS.seek 0.510ml @23:54:13.08 delay 2 seconds @23:54:15.09 ATV.dial :bleach @23:54:15.58 AS.seek 0.810ml @23:55:17.01 delay 3 seconds @23:55:20.02 ATV.dial :air3 @23:55:20.34 delay 3 seconds @23:55:23.40 AS.seek 0.835ml @23:55:28.67 delay 3 seconds @23:55:31.68 ATV.dial :bleach @23:55:32.08 AS.seek 0.820ml @23:55:35.35 delay 3 seconds @23:55:38.36 ARV.dial :waste @23:55:38.86 delay 3 seconds @23:55:42.42 ATV.dial :ARV @23:55:43.15 AS.reconfigure ASconfig @23:55:43.18 AS.seek 0.470ml @23:56:03.87 AFV.select :carrierSolution @23:56:03.97 delay 2 seconds @23:56:05.98 AS.seek 1.000ml @23:56:37.20 delay 1 second @23:56:38.21 AFV.select :holdingCoil @23:56:38.30 delay 1 second @23:56:39.31 AS.reconfigure ASfast @23:56:39.34 AS.seek 0.000ml @23:57:07.06 AFV.select :carrierSolution @23:57:07.16 delay 2 seconds @23:57:09.17 AS.reconfigure ASconfig @23:57:09.20 AS.seek 1.000ml @23:58:07.80 delay 1 second @23:58:08.81 AFV.select :holdingCoil @23:58:08.90 delay 1 second @23:58:09.91 AS.reconfigure ASfast @23:58:09.94 AS.seek 0.000ml @23:58:37.68 AFV.select :carrierSolution @23:58:37.79 delay 2 seconds @23:58:39.80 AS.reconfigure ASconfig @23:58:39.83 AS.seek 1.000ml @23:59:38.41 delay 1 second @23:59:39.42 AFV.select :holdingCoil @23:59:39.51 delay 1 second @23:59:40.52 AS.reconfigure ASfast @23:59:40.55 AS.seek 0.000ml @00:00:08.28PST15-Feb-14 ARV.dial between carrier and air1 @00:00:08.69 ATV.dial :air3 @00:00:09.63 AS.reconfigure ASslow1 @00:00:09.66 AS.seek 0.010ml @00:00:11.99 delay 2 seconds @00:00:14.00 ATV.dial :bleach @00:00:14.40 AS.seek 0.810ml @00:02:57.81 delay 3 seconds @00:03:00.82 ATV.dial :air3 @00:03:01.17 delay 3 seconds @00:03:04.23 AS.seek 0.835ml @00:03:09.58 delay 3 seconds @00:03:12.59 ATV.dial :bleach @00:03:13.08 AS.seek 0.820ml @00:03:16.42 delay 3 seconds @00:03:19.43 ATV.dial PCR @00:03:19.88 AS.reconfigure ASconfig @00:03:19.91 AS.seek empty @00:04:08.07 PCR.program :bleachProfile @00:04:08.12 PCR program should complete in < 2:45 @00:04:08.13 PCR.run 10:00 @00:07:09.34 PCR.off @00:07:09.37 AFV.select :carrierSolution @00:07:09.46 delay 2 seconds @00:07:11.47 AS.seek 1.000ml @00:08:10.13 delay 1 second @00:08:11.14 AFV.select :holdingCoil @00:08:11.23 delay 1 second @00:08:12.24 AS.reconfigure ASfast @00:08:12.27 AS.seek 0.000ml @00:08:39.96 delay 3 seconds @00:08:42.97 AFV.select :carrierSolution @00:08:43.06 delay 2 seconds @00:08:45.07 AS.reconfigure ASconfig @00:08:45.10 AS.seek 1.000ml @00:09:43.70 delay 1 second @00:09:44.71 AFV.select :holdingCoil @00:09:44.80 delay 1 second @00:09:45.81 AS.reconfigure ASfast @00:09:45.84 AS.seek 0.000ml @00:10:13.56 delay 3 seconds @00:10:16.57 AFV.select :carrierSolution @00:10:16.66 delay 2 seconds @00:10:18.68 AS.reconfigure ASconfig @00:10:18.71 AS.seek 1.000ml @00:11:17.32 delay 1 second @00:11:18.33 AFV.select :holdingCoil @00:11:18.42 delay 1 second @00:11:19.43 AS.reconfigure ASfast @00:11:19.46 AS.seek 0.000ml @00:11:47.19 delay 3 seconds @00:11:50.20 AFV.select :carrierSolution @00:11:50.29 delay 2 seconds @00:11:52.30 AS.reconfigure ASconfig @00:11:52.33 AS.seek 1.000ml @00:12:50.96 delay 1 second @00:12:51.97 AFV.select :holdingCoil @00:12:52.06 delay 1 second @00:12:53.07 AS.reconfigure ASfast @00:12:53.10 AS.seek 0.000ml @00:13:20.79 delay 3 seconds @00:13:23.80 AFV.select :carrierSolution @00:13:23.89 delay 2 seconds @00:13:25.90 AS.reconfigure ASconfig @00:13:25.93 AS.seek 1.000ml @00:14:24.53 delay 1 second @00:14:25.54 AFV.select :holdingCoil @00:14:25.63 delay 1 second @00:14:26.64 AS.reconfigure ASnorm @00:14:26.67 AS.seek 0.000ml @00:15:18.12 Setting up PCR for IPCem -- writing /var/log/esp/IPCem14feb1500h700ml.pcr @00:15:18.13 Building PCR Slug from ASV with MM3 mix, PP5 primer @00:15:18.14 Priming with carrier ATV.dial ARV @00:15:18.67 ARV.dial :carrier @00:15:18.96 AS.seek 0.500ml @00:15:44.84 AFV.select :carrierSolution @00:15:44.93 delay 2 seconds @00:15:46.94 AS.reconfigure ASconfig @00:15:46.97 AS.seek 1.000ml @00:16:16.44 delay 1 second @00:16:17.45 AFV.select :holdingCoil @00:16:17.55 delay 1 second @00:16:18.56 ATV.dial :waste @00:16:19.85 AS.reconfigure ASnorm @00:16:19.88 AS.seek empty @00:17:11.37 AFV.select :carrierSolution @00:17:11.47 delay 2 seconds @00:17:13.48 AS.reconfigure ASconfig @00:17:13.51 AS.seek 0.500ml @00:17:43.00 delay 1 second @00:17:44.01 AFV.select :holdingCoil @00:17:44.10 Building Slug @00:17:44.11 ATV.dial :ARV @00:17:45.37 delay 2 seconds @00:17:47.38 ARV.dial :air1 @00:17:47.70 delay 2 seconds @00:17:49.76 AS.reconfigure ASslow2 @00:17:49.79 AS.seek 0.506ml @00:17:56.47 delay 2 seconds @00:17:58.48 ARV.dial :MM3 @00:17:59.84 delay 2 seconds @00:18:01.85 Adding MM3 @00:18:01.91 AS.seek 0.524ml @00:18:20.62 delay 25 seconds @00:18:45.63 ARV.dial :PP5 @00:18:45.97 Adding PP5 @00:18:45.98 delay 2 seconds @00:18:48.03 AS.seek 0.530ml @00:18:54.59 delay 10 seconds @00:19:04.60 ARV.dial :air3 @00:19:04.92 adding ARV air @00:19:04.93 delay 2 seconds @00:19:06.99 AS.seek 0.536ml @00:19:13.65 delay 5 seconds @00:19:18.66 ARV.dial :carrier @00:19:19.70 delay 7 seconds @00:19:26.76 AS.seek 0.594ml @00:20:26.34 delay 5 seconds @00:20:31.35 ATV.dial :ASV @00:20:31.68 delay 2 seconds @00:20:33.74 AS.seek 0.600ml @00:20:40.02 delay 4 seconds @00:20:44.03 ATV.dial ARV @00:20:44.46 delay 2 seconds @00:20:46.47 ARV.dial :carrier @00:20:46.48 delay 2 seconds @00:20:48.53 AS.seek 0.650ml @00:21:39.65 delay 4 seconds @00:21:43.66 ATV.dial PCR @00:21:44.18 ASV.dial :waste1 @00:21:44.19 ARV.dial :air1 @00:21:44.53 Positioning Slug by 0.324ml @00:21:44.58 AS.seek 0.326ml @00:27:15.28 delay 5 seconds @00:27:20.29 ATV.dial ARV @00:27:20.82 ASV.dial :PCRlock @00:27:21.30 delay 5 seconds @00:27:26.31 Running IPCem ProfileEM59C thermocycling @00:27:26.32 PCR.program ProfileEM59C @00:27:26.43 ProfileEM59C should complete in < 2:11:38 @00:27:26.44 PCR.run 2:49:32.5 @02:21:37.15 PCR.off @02:21:37.69 PCR for IPCem completed -- closed /var/log/esp/IPCem14feb1500h700ml.pcr @02:21:37.70 Full MFB Decontamination @02:21:37.71 Decon extraction column and decon AIV @02:21:37.73 SPE.hold 2:46:40,55.0C @02:21:37.74 ASV.dial :SPEdelivery @02:21:38.70 delay 20 seconds @02:21:58.71 AFV.select :carrierSolution @02:21:58.81 delay 2 seconds @02:22:00.82 AS.reconfigure ASconfig @02:22:00.85 AS.seek 0.500ml @02:22:11.30 delay 1 second @02:22:12.31 AFV.select :holdingCoil @02:22:12.41 ATV.dial :air1 @02:22:13.92 AS.reconfigure ASslow1 @02:22:13.95 AS.seek 0.520ml @02:22:18.28 ATV.dial :ASV @02:22:19.56 ASV.dial :SPErecovery @02:22:20.27 AS.seek 0.620ml @02:22:40.90 ATV.dial :waste @02:22:42.45 AS.reconfigure ASnorm @02:22:42.48 AS.seek empty @02:23:14.53 AFV.select :holdingCoil @02:23:14.54 ATV.dial :waste @02:23:14.55 AS.reconfigure ASconfig @02:23:14.58 AS.seek 0.000ml @02:23:14.89 AFV.select :carrierSolution @02:23:15.00 delay 2 seconds @02:23:17.01 AS.seek 0.500ml @02:23:46.42 delay 1 second @02:23:47.43 AFV.select :holdingCoil @02:23:47.53 ATV.dial :air3 @02:23:48.09 AS.reconfigure ASslow1 @02:23:48.12 AS.seek 0.520ml @02:23:52.47 ATV.dial :air3 @02:23:52.53 AS.seek 0.530ml @02:23:54.79 delay 2 seconds @02:23:56.80 ATV.dial :bleach @02:23:57.17 AS.seek 0.710ml @02:24:34.10 delay 3 seconds @02:24:37.11 ATV.dial :air3 @02:24:37.53 delay 3 seconds @02:24:40.59 AS.seek 0.735ml @02:24:45.93 delay 3 seconds @02:24:48.94 ATV.dial :bleach @02:24:49.31 AS.seek 0.720ml @02:24:52.62 delay 3 seconds @02:24:55.63 ATV.dial ASV @02:24:56.53 ASV.dial :SPErecovery @02:24:56.59 AS.seek 0.365ml @02:26:09.24 ATV.dial :waste @02:26:10.71 AS.reconfigure ASnorm @02:26:10.74 AS.seek empty @02:26:29.76 ATV.dial :air3 @02:26:30.32 AS.reconfigure ASslow1 @02:26:30.35 AS.seek 0.009ml @02:26:32.65 delay 2 seconds @02:26:34.66 ATV.dial :bleach @02:26:35.04 AS.seek 0.579ml @02:28:31.55 delay 3 seconds @02:28:34.56 ATV.dial :air3 @02:28:34.98 delay 3 seconds @02:28:38.04 AS.seek 0.604ml @02:28:43.39 delay 3 seconds @02:28:46.40 ATV.dial :bleach @02:28:46.78 AS.seek 0.589ml @02:28:50.11 delay 3 seconds @02:28:53.12 ATV.dial :ASV @02:28:54.01 ASV.dial :wasteR @02:28:54.77 AS.seek 0.000ml @02:30:55.20 delay 1:00 @02:31:55.21 ATV.dial ASV @02:31:55.22 AFV.select :carrierSolution @02:31:55.32 delay 2 seconds @02:31:57.33 AS.reconfigure ASconfig @02:31:57.36 AS.seek 1.000ml @02:32:55.97 delay 1 second @02:32:56.98 AFV.select :holdingCoil @02:32:57.07 delay 1 second @02:32:58.08 ASV.dial :wasteR @02:32:58.09 AS.reconfigure ASslow1 @02:32:58.12 AS.seek 0.000ml @02:36:22.48 delay 10 seconds @02:36:32.49 ATV.dial :air3 @02:36:33.61 AS.seek 0.010ml @02:36:35.89 delay 2 seconds @02:36:37.90 ATV.dial :bleach @02:36:38.39 AS.seek 0.580ml @02:38:34.81 delay 3 seconds @02:38:37.82 ATV.dial :air3 @02:38:38.15 delay 3 seconds @02:38:41.21 AS.seek 0.605ml @02:38:46.60 delay 3 seconds @02:38:49.61 ATV.dial :bleach @02:38:50.01 AS.seek 0.590ml @02:38:53.34 delay 3 seconds @02:38:56.35 ATV.dial ASV @02:38:57.23 ASV.dial :SPEdelivery @02:38:57.96 AS.seek 0.000ml @02:40:58.65 delay 1:00 @02:41:58.66 AFV.select :carrierSolution @02:41:58.76 delay 2 seconds @02:42:00.77 AS.reconfigure ASconfig @02:42:00.80 AS.seek 1.000ml @02:42:59.35 delay 1 second @02:43:00.36 AFV.select :holdingCoil @02:43:00.46 delay 1 second @02:43:01.47 ATV.dial ASV @02:43:01.48 ASV.dial :SPEdelivery @02:43:01.49 AS.reconfigure ASslow1 @02:43:01.52 AS.seek 0.000ml @02:46:25.77 delay 10 seconds @02:46:35.78 ATV.dial :air2 @02:46:36.50 AS.reconfigure ASslow0 @02:46:36.53 AS.seek 0.800ml @02:47:58.47 delay 3 seconds @02:48:02.02 ATV.dial :ASV @02:48:02.74 ASV.dial :SPEdelivery @02:48:02.76 AS.reconfigure ASslow1 @02:48:02.79 AS.seek 0.030ml @02:50:40.09 delay 20 seconds @02:51:00.10 ATV.dial :waste,via => air3 @02:51:01.56 AS.reconfigure ASconfig @02:51:01.59 AS.seek empty @02:51:03.69 SPE.coast @02:51:03.77 Removing Bleach from Mixing Coil @02:51:03.78 AFV.select :carrierSolution @02:51:03.87 delay 2 seconds @02:51:05.88 AS.seek 0.500ml @02:51:35.36 delay 1 second @02:51:36.37 AFV.select :holdingCoil @02:51:36.46 ATV.dial :ASV @02:51:37.90 ASV.dial :SPErecovery @02:51:38.47 AS.reconfigure ASslow1 @02:51:38.50 AS.seek 0.730ml @02:52:25.67 ASV.dial :wasteD @02:52:26.18 AS.reconfigure ASnorm @02:52:26.21 AS.seek 0.000ml @02:53:03.84 AFV.select :carrierSolution @02:53:03.94 delay 2 seconds @02:53:05.95 AS.reconfigure ASconfig @02:53:05.98 AS.seek 0.500ml @02:53:35.42 delay 1 second @02:53:36.43 AFV.select :holdingCoil @02:53:36.52 ATV.dial ASV @02:53:36.53 ASV.dial :SPErecovery @02:53:37.23 AS.reconfigure ASslow0 @02:53:37.26 AS.seek 0.300ml @02:53:57.96 ATV.dial :air2 @02:53:58.73 AS.reconfigure ASslow1 @02:53:58.76 AS.seek 0.320ml @02:54:03.15 ATV.dial ASV @02:54:03.92 AS.reconfigure ASslow0 @02:54:03.95 AS.seek 0.570ml @02:54:29.82 ASV.dial :wasteD @02:54:30.49 AS.reconfigure ASnorm @02:54:30.52 AS.seek 0.000ml @02:54:59.98 AFV.select :carrierSolution @02:55:00.08 delay 2 seconds @02:55:02.09 AS.reconfigure ASconfig @02:55:02.12 AS.seek 0.500ml @02:55:31.54 delay 1 second @02:55:32.55 AFV.select :holdingCoil @02:55:32.64 ATV.dial ASV @02:55:32.65 ASV.dial :SPErecovery @02:55:33.35 AS.reconfigure ASslow0 @02:55:33.38 AS.seek 0.300ml @02:55:54.07 ATV.dial :air2 @02:55:54.84 AS.reconfigure ASslow1 @02:55:54.87 AS.seek 0.320ml @02:55:59.24 ATV.dial ASV @02:56:00.01 AS.reconfigure ASslow0 @02:56:00.04 AS.seek 0.570ml @02:56:25.77 ASV.dial :wasteD @02:56:26.44 AS.reconfigure ASnorm @02:56:26.47 AS.seek 0.000ml @02:56:55.91 ATV.dial :air2 @02:56:56.64 AS.reconfigure ASslow0 @02:56:56.67 AS.seek 0.300ml @02:57:27.61 ATV.dial :ASV @02:57:28.33 ASV.dial :wasteD @02:57:28.35 AS.reconfigure ASconfig @02:57:28.38 AS.seek 0.050ml @02:57:43.25 ATV.dial :waste @02:57:44.70 AS.seek 0.000ml @02:57:47.89 ASV.dial :SPEdelivery @02:57:48.91 deconLysateINT @02:57:48.92 ATV.dial :air3 @02:57:49.49 AS.reconfigure ASslow1 @02:57:49.52 AS.seek 0.010ml @02:57:51.86 delay 2 seconds @02:57:53.87 ATV.dial :bleach @02:57:54.24 AS.seek 0.960ml @03:01:08.22 delay 3 seconds @03:01:11.23 ATV.dial :air3 @03:01:11.57 delay 3 seconds @03:01:14.63 AS.seek 0.985ml @03:01:19.96 delay 3 seconds @03:01:22.97 ATV.dial :bleach @03:01:23.37 AS.seek 0.970ml @03:01:26.77 delay 3 seconds @03:01:29.78 ATV.dial :AIV @03:01:30.94 AIV.dial :coilToMFB @03:01:30.95 AWV.select :waste @03:01:30.96 AFV.select :carrierSolution @03:01:31.05 delay 2 seconds @03:01:33.06 AS.reconfigure ASconfig @03:01:33.09 AS.seek 1.000ml @03:01:35.10 delay 1 second @03:01:36.11 AFV.select :holdingCoil @03:01:36.21 delay 1 second @03:01:37.22 AS.reconfigure ASslow0 @03:01:37.25 AS.seek empty @03:03:19.64 AFV.select :carrierSolution @03:03:19.73 delay 2 seconds @03:03:21.74 AS.reconfigure ASconfig @03:03:21.77 AS.seek 1.000ml @03:04:20.44 delay 1 second @03:04:21.45 AFV.select :holdingCoil @03:04:21.55 delay 1 second @03:04:22.56 AS.reconfigure ASslow0 @03:04:22.59 AS.seek empty @03:06:05.00 AFV.select :carrierSolution @03:06:05.10 delay 2 seconds @03:06:07.11 AS.reconfigure ASconfig @03:06:07.14 AS.seek 1.000ml @03:07:05.79 delay 1 second @03:07:06.80 AFV.select :holdingCoil @03:07:06.90 delay 1 second @03:07:07.91 AS.reconfigure ASslow0 @03:07:07.94 AS.seek empty @03:08:50.35 ATV.dial :air2 @03:08:50.93 AS.reconfigure ASslow1 @03:08:50.96 AS.seek 0.009ml @03:08:53.29 AS.reconfigure ASslow0 @03:08:53.33 AS.seek 1.000ml @03:10:34.66 ATV.dial AIV @03:10:35.30 AS.seek 0.020ml @03:12:16.11 ATV.dial :waste @03:12:17.39 AS.seek 0.000ml @03:12:19.74 AWV.select :air @03:12:19.89 Cleaning PCR AFV.select :carrierSolution @03:12:19.98 delay 2 seconds @03:12:21.99 AS.reconfigure ASconfig @03:12:22.02 AS.seek 0.500ml @03:12:51.47 delay 1 second @03:12:52.48 AFV.select :holdingCoil @03:12:52.57 ASV.dial :waste1 @03:12:52.91 ATV.dial :air3 @03:12:53.49 AS.reconfigure ASslow1 @03:12:53.52 AS.seek 0.510ml @03:12:55.84 delay 2 seconds @03:12:57.85 ATV.dial :bleach @03:12:58.22 AS.seek 0.810ml @03:13:59.67 delay 3 seconds @03:14:02.68 ATV.dial :air3 @03:14:03.03 delay 3 seconds @03:14:06.09 AS.seek 0.835ml @03:14:11.44 delay 3 seconds @03:14:14.45 ATV.dial :bleach @03:14:14.85 AS.seek 0.820ml @03:14:18.19 delay 3 seconds @03:14:21.20 ARV.dial :waste @03:14:21.70 delay 3 seconds @03:14:24.71 ATV.dial :ARV @03:14:25.44 AS.reconfigure ASconfig @03:14:25.48 AS.seek 0.470ml @03:14:46.17 AFV.select :carrierSolution @03:14:46.27 delay 2 seconds @03:14:48.28 AS.seek 1.000ml @03:15:19.50 delay 1 second @03:15:20.51 AFV.select :holdingCoil @03:15:20.60 delay 1 second @03:15:21.61 AS.reconfigure ASfast @03:15:21.64 AS.seek 0.000ml @03:15:49.37 AFV.select :carrierSolution @03:15:49.47 delay 2 seconds @03:15:51.48 AS.reconfigure ASconfig @03:15:51.51 AS.seek 1.000ml @03:16:50.09 delay 1 second @03:16:51.10 AFV.select :holdingCoil @03:16:51.19 delay 1 second @03:16:52.20 AS.reconfigure ASfast @03:16:52.23 AS.seek 0.000ml @03:17:19.95 AFV.select :carrierSolution @03:17:20.05 delay 2 seconds @03:17:22.06 AS.reconfigure ASconfig @03:17:22.09 AS.seek 1.000ml @03:18:20.72 delay 1 second @03:18:21.73 AFV.select :holdingCoil @03:18:21.82 delay 1 second @03:18:22.83 AS.reconfigure ASfast @03:18:22.86 AS.seek 0.000ml @03:18:50.56 ARV.dial between carrier and air1 @03:18:51.08 ATV.dial :air3 @03:18:52.13 AS.reconfigure ASslow1 @03:18:52.16 AS.seek 0.010ml @03:18:54.48 delay 2 seconds @03:18:56.49 ATV.dial :bleach @03:18:56.86 AS.seek 0.810ml @03:21:40.26 delay 3 seconds @03:21:43.27 ATV.dial :air3 @03:21:43.60 delay 3 seconds @03:21:46.66 AS.seek 0.835ml @03:21:52.04 delay 3 seconds @03:21:55.05 ATV.dial :bleach @03:21:55.42 AS.seek 0.820ml @03:21:58.76 delay 3 seconds @03:22:01.77 ATV.dial PCR @03:22:02.11 AS.reconfigure ASconfig @03:22:02.14 AS.seek empty @03:22:50.26 PCR.program :bleachProfile @03:22:50.32 PCR program should complete in < 2:45 @03:22:50.33 PCR.run 10:00 @03:25:57.58 PCR.off @03:25:57.61 AFV.select :carrierSolution @03:25:57.70 delay 2 seconds @03:25:59.71 AS.seek 1.000ml @03:26:58.36 delay 1 second @03:26:59.37 AFV.select :holdingCoil @03:26:59.46 delay 1 second @03:27:00.47 AS.reconfigure ASfast @03:27:00.50 AS.seek 0.000ml @03:27:28.23 delay 3 seconds @03:27:31.24 AFV.select :carrierSolution @03:27:31.33 delay 2 seconds @03:27:33.34 AS.reconfigure ASconfig @03:27:33.37 AS.seek 1.000ml @03:28:31.98 delay 1 second @03:28:32.99 AFV.select :holdingCoil @03:28:33.08 delay 1 second @03:28:34.09 AS.reconfigure ASfast @03:28:34.12 AS.seek 0.000ml @03:29:01.86 delay 3 seconds @03:29:04.87 AFV.select :carrierSolution @03:29:04.96 delay 2 seconds @03:29:06.97 AS.reconfigure ASconfig @03:29:07.00 AS.seek 1.000ml @03:30:05.60 delay 1 second @03:30:06.61 AFV.select :holdingCoil @03:30:06.70 delay 1 second @03:30:07.71 AS.reconfigure ASfast @03:30:07.74 AS.seek 0.000ml @03:30:35.48 delay 3 seconds @03:30:38.49 AFV.select :carrierSolution @03:30:38.58 delay 2 seconds @03:30:40.59 AS.reconfigure ASconfig @03:30:40.62 AS.seek 1.000ml @03:31:39.21 delay 1 second @03:31:40.22 AFV.select :holdingCoil @03:31:40.31 delay 1 second @03:31:41.32 AS.reconfigure ASfast @03:31:41.35 AS.seek 0.000ml @03:32:09.09 delay 3 seconds @03:32:12.10 AFV.select :carrierSolution @03:32:12.19 delay 2 seconds @03:32:14.20 AS.reconfigure ASconfig @03:32:14.23 AS.seek 1.000ml @03:33:12.86 delay 1 second @03:33:13.87 AFV.select :holdingCoil @03:33:13.96 delay 1 second @03:33:14.97 AS.reconfigure ASnorm @03:33:15.52 AS.seek 0.000ml @03:34:06.96 AFV.select :carrierSolution @03:34:07.05 delay 2 seconds @03:34:09.06 AS.reconfigure ASconfig @03:34:09.09 AS.seek 1.000ml @03:35:07.71 delay 1 second @03:35:08.72 AFV.select :holdingCoil @03:35:08.81 delay 1 second @03:35:09.82 ATV.dial :waste @03:35:10.72 AS.reconfigure ASnorm @03:35:10.75 AS.seek 0.500ml @03:35:36.68 ATV.dial :waste2 @03:35:37.79 AS.seek 0.000ml @03:36:03.66 AS.stop @03:36:03.68 CC.stop @03:36:03.71 CS.stop @03:36:03.74 PC.stop @03:36:03.77 PS.stop @03:36:03.80 Forearm.stop @03:36:03.83 Elbow.stop @03:36:03.86 SS.stop @03:36:03.93 Sleepy.power :raw,:OFF @03:36:03.94 Sleepy.power :dwarf,:OFF Shutdown @03:36:03.95 GoodNews.email "MFBPCR completed",Subject => "Shutdown" @08:00:00.33
Powering up Dwarves @08:00:05.34 Sleepy.power :raw,:ON @08:00:05.80 SPE.configure SPEconfig @08:00:05.82 AS.configure ASconfig @08:00:05.85 CC.configure CCconfig @08:00:05.88 CS.configure CSconfig @08:00:05.91 PC.configure PCconfig @08:00:05.94 PS.configure PSconfig @08:00:05.97 Forearm.configure LooseForearm @08:00:06.00 Elbow.configure LooseElbow @08:00:06.03 SC.configure SCloose @08:00:06.06 SE.configure SEconfig @08:00:06.09 SS.configure SSconfig @08:00:06.12 CH.configure CHconfig @08:00:06.14 PH.configure PHconfig @08:00:06.17 Valve.reset @08:00:06.84 PCR.off @08:00:06.87 PCR.pidtemp= 50,0.1,0,300,25,25 @08:00:06.90 PCR.caltemp= 6.6661,0.0344,0.0 @08:00:06.99 Intake.select :close @08:00:07.02 Exhaust.select :close @08:00:07.03 CTV.dial :air @08:00:07.08 PSV.dial :air @08:00:07.10
PC.reconfigure PCconfig @08:00:07.16 PC.home.seek open @08:00:07.18 CSV.dial :waste1 @08:00:07.58 PS.reconfigure PSconfig @08:00:07.61 PS.home.seek 0.000ml @08:00:07.66 SSV.dial :spit,avoiding => exhaust @08:00:08.44 CS.reconfigure CSfast @08:00:08.47 SS.reconfigure SSconfig @08:00:08.56 SS.home.seek 0.00ml @08:00:08.58 CS.home.seek 0.000ml @08:00:08.66 CC.reconfigure CCconfig @08:00:08.69 CC.home.seek open @08:00:17.85 PS.coast @08:00:17.95
CC.seek open @08:00:17.97 PC.seek open @08:00:18.11
Forearm.reconfigure LooseForearm @08:00:18.14 Forearm.home.seek retract @08:00:18.89 Hand.grip @08:00:18.91
Elbow.jog -1500 @08:00:19.42 Elbow.reconfigure LooseElbow @08:00:19.46 Elbow.home.seek clear @08:00:24.17 SE.reconfigure SEconfig @08:00:24.20 SE.home.seek down @08:00:24.77 SC.reconfigure SCsloppy @08:00:24.81 SC.home.seek initial @08:00:27.08 Forearm.seek retract @08:00:27.14 Elbow.seek hammer @08:00:28.33 Forearm.reconfigure TightForearm @08:00:28.38 Forearm.seek 2 @08:00:29.49 Forearm.seek retract @08:00:31.05 Forearm.reconfigure LooseForearm @08:00:31.38PST15-Feb-14 ===> STARTED wqPCR (phase #2) <=== @08:00:31.39 Starting initialPurge @08:00:31.40 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:00:31.45 Hand.release @08:00:32.29 Elbow.reconfigure TightElbow @08:00:32.33 Elbow.seek FlushPuck::Garage @08:00:34.10 Elbow.reconfigure LooseElbow @08:00:34.19 Forearm.seek garage @08:00:35.22 Hand.grip @08:00:36.04 Forearm.seek retract @08:00:37.05 Elbow.reconfigure TightElbow @08:00:37.09 Elbow.seek CC @08:00:39.90 Elbow.reconfigure LooseElbow @08:00:39.99 Forearm.reconfigure TightForearm @08:00:40.02 Forearm.seek CC @08:00:41.14 CC.seek closed @08:00:44.88 Collection Clamp onSpring/offSpring for 5/22 samples, on/off torque 1.91 @08:00:44.93 Hand.release @08:00:45.75 Forearm.reconfigure LooseForearm @08:00:45.78 Forearm.seek retract @08:00:46.75 Flushing the Collection Valve::Manifold @08:00:46.84 CS Pulling 5ml of flush @08:00:46.85 CSV.dial CSR @08:00:48.11 CSR.select :flush @08:00:48.21 CS.reconfigure CSconfig @08:00:48.25 CS.seek 5.000ml @08:01:01.69 delay 5 seconds @08:01:06.70 CSR.select :air @08:01:06.84 CS.reconfigure CSfast @08:01:06.87 CS.seek 7.500ml @08:01:13.53 CSV.dial :waste @08:01:14.81 CS.reconfigure CSconfig @08:01:14.84 CS.seek 0.000ml @08:01:34.66 Purging external Intake @08:01:34.75 CSV.dial :flush @08:01:35.18 CS.reconfigure CSfast @08:01:35.21 CS.seek 10.000ml @08:01:58.81 delay 5 seconds @08:02:03.82 CSV.dial CBV @08:02:03.84
CBV.dial CSV @08:02:04.63 CTV.dial :intake @08:02:05.38 Intake.select :open @08:02:05.47 CS.seek 0.000ml @08:02:29.56 Intake.select :close @08:02:29.65 CTV.dial :air @08:02:30.39 Flushing upper manifold @08:02:30.48 CSV.dial :flush @08:02:31.28 CS.seek 5.000ml @08:02:43.59 delay 5 seconds @08:02:48.60 CSV.dial :air @08:02:49.06 CS.seek 7.000ml @08:02:54.47 CSV.dial CBV @08:02:54.49
CBV.dial CSV @08:02:55.56 CTV.dial :air @08:02:55.58 CS.seek 0.000ml @08:03:12.25 delay 7 seconds @08:03:19.27 CS.seek 3.000ml @08:03:26.97 CSV.dial :waste @08:03:27.48 CS.seek 0.000ml @08:03:35.23 CSV.dial :flush @08:03:35.66 CS.seek 3.000ml @08:03:43.31 delay 5 seconds @08:03:48.33 CSV.dial :air @08:03:48.81 CS.seek 5.000ml @08:03:54.28 CSV.dial :air @08:03:54.29 CS.seek 0.000ml @08:04:06.46 delay 7 seconds @08:04:13.48 CS.seek 3.000ml @08:04:21.12 CSV.dial :waste @08:04:21.93 CS.seek 0.000ml @08:04:29.59 Garaging FlushPuck in Collection Clamp @08:04:29.65 Elbow.reconfigure TightElbow @08:04:29.68 Elbow.seek CC @08:04:29.88 Elbow.reconfigure LooseElbow @08:04:29.97 Forearm.seek CC @08:04:30.92 CC.seek sealed @08:04:31.84 Hand.grip @08:04:32.58 CC.seek open @08:04:35.87 Forearm.seek retract @08:04:36.80 Elbow.reconfigure TightElbow @08:04:36.84 Elbow.seek garage @08:04:39.59 Elbow.reconfigure LooseElbow @08:04:39.68 Forearm.reconfigure TightForearm @08:04:40.26 Forearm.seek garage @08:04:41.41 Hand.release @08:04:42.24 Forearm.reconfigure LooseForearm @08:04:42.27 Forearm.seek retract @08:04:43.38 Starting mfb @08:04:43.39 pcrBAC sampling at most 1000ml, wcr at most 1000ml @08:04:43.40 BACfans.email "Can@08:00:31, 12.9C, 62% humidity, 14.0psia, 13.739V, 0.598A, 0.591A avg, 8.21W\nCTD data unavailable\nISUS data unavailable",Subject => "pcrBAC sampling at most 1000ml, wcr at most 1000ml" @08:04:43.43
Loading sh1 puck from tube 2 into Collection Clamp @08:04:50.53 Readying the MFB @08:04:50.56 Homing MFB Syringe AFV.select :holdingCoil @08:04:50.76
SC.reconfigure SCloose @08:04:50.81 ATV.dial :air2 @08:04:50.99
Elbow.reconfigure TightElbow @08:04:51.02 Elbow.seek 2 @08:04:51.21 AS.reconfigure ASconfig @08:04:51.24 AS.home.seek home @08:04:52.89
Forearm.reconfigure TightForearm @08:04:52.92 Forearm.seek 2 + 800 counts @08:04:53.99 SE.reconfigure SEpick @08:04:54.04 SE.seek beamBreak @08:04:55.53 SE.configure SEconfig @08:04:55.68 Elbow.seek 2 @08:04:55.81 Forearm.seek 2 + 300 counts @08:04:56.02 Forearm.reconfigure LooseForearm @08:04:56.06 Hand.grip @08:04:56.85 SE.seek down @08:04:57.00
Forearm.seek retract @08:04:57.97 Elbow.seek CC @08:04:58.10 SE.coast @08:04:58.73 AFV.select :carrierSolution @08:04:58.83 delay 2 seconds @08:04:59.51
Elbow.reconfigure LooseElbow @08:04:59.59 Forearm.reconfigure TightForearm @08:04:59.62 Forearm.seek CC @08:05:00.81 CC.seek closed @08:05:00.85 AS.seek 1.000ml @08:05:04.62
Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.93 @08:05:04.63 Hand.release @08:05:05.37 Forearm.reconfigure LooseForearm @08:05:05.40 Forearm.seek retract @08:05:06.33 SC.coast @08:05:06.36 collectSample 1000ml w/bubble point of 55psi Priming 7ml @08:05:06.37 Sleepy.power :sampler,:ON @08:05:06.38 Intake.select :close Exhaust.select :close @08:05:06.39 SS.hold @08:05:06.55 Calibrating pressure and temperature sensors @08:05:06.57 CTV.dial :air @08:05:06.58 SSV.dial :spit,avoiding => exhaust @08:05:06.59 SS.reconfigure SSfast @08:05:06.62 SS.seek 5.00ml @08:05:09.38 SSV.dial CBV @08:05:09.40
CBV.dial SSV @08:05:10.75 delay 3.8 seconds @08:05:14.63 SSV.dial :spit @08:05:15.95 SS.seek 0.00ml @08:05:18.57 SSV.dial CBV @08:05:19.90 CTV.dial :intake @08:05:20.65 Waiting for external sampler to finish priming... @08:05:20.66 delayUntil "08:15:06.37" @08:05:52.59 delay 1 second @08:05:53.60 AFV.select :holdingCoil @08:05:53.69 delay 1 second @08:05:54.70 ATV.dial :waste @08:05:55.69 AS.reconfigure ASnorm @08:05:55.72 AS.home.seek empty @08:06:48.88 ATV.dial :waste @08:06:48.89 ARV.dial between carrier and air1 @08:06:48.96 ASV.dial :waste1 @08:15:06.38 SSV.dial CBV @08:15:06.40
CBV.dial SSV @08:15:06.41 Allowing 55s for 7.0ml of remaining 7.0ml Intake.select :open @08:15:08.54 Intake @45.9psia, vacuum from 20.0 to 24.0psi @08:15:08.55 SS.reconfigure forSampling @08:15:08.59 SS.seek 7.00ml @08:15:11.46 Vacuum too high -- waiting up to 40s @08:15:11.72 Intake @40.0psia, vacuum from 20.0 to 24.0psi @08:15:11.73 SS.reconfigure forSampling @08:15:11.76 SS.seek 7.00ml @08:15:15.13 Stroke completed -- waiting for pressure to equalize ... @08:15:15.78 Intake.select :close @08:15:15.91 SSV.dial :exhaust @08:15:16.66 Exhaust.select :open @08:15:16.76 SS.reconfigure SSexhale @08:15:16.80 SS.seek 0.00ml @08:15:20.75 Exhaust.select :close @08:15:20.85 Sampling 1000.0ml @08:15:20.86 Intake.select :close @08:15:20.87 Exhaust.select :close @08:15:20.88 SS.reconfigure SSconfig @08:15:20.91 SS.hold @08:15:21.06 CTV.dial :intake @08:15:21.07 SSV.dial CBV @08:15:21.09
CBV.dial SSV @08:15:21.87 Allowing 185s for 25.0ml of remaining 1000.0ml Intake.select :open @08:15:24.00 Intake @46.8psia, vacuum from 20.0 to 24.0psi @08:15:24.01 SS.reconfigure forSampling @08:15:24.05 SS.seek 25.00ml @08:15:28.08 Vacuum too high -- waiting up to 40s @08:15:28.35 Intake @39.7psia, vacuum from 20.0 to 24.0psi @08:15:28.36 SS.reconfigure forSampling @08:15:28.39 SS.seek 25.00ml @08:15:42.11 Stroke completed -- waiting for pressure to equalize ... @08:15:42.75 Intake.select :close @08:15:42.88 SSV.dial :exhaust @08:15:43.63 Exhaust.select :open @08:15:43.72 SS.reconfigure SSexhale @08:15:43.76 SS.seek 0.00ml @08:15:52.73 Exhaust.select :close @08:15:52.82 SSV.dial CBV @08:15:52.84
CBV.dial SSV @08:15:53.61 Allowing 185s for 25.0ml of remaining 975.0ml @08:15:53.62 Intake.select :open @08:15:55.77 Intake @47.1psia, vacuum from 20.0 to 24.0psi @08:15:55.78 SS.reconfigure forSampling @08:15:55.82 SS.seek 25.00ml @08:16:07.20 Vacuum too high -- waiting up to 40s @08:16:07.47 Intake @41.3psia, vacuum from 20.0 to 24.0psi @08:16:07.48 SS.reconfigure forSampling @08:16:07.51 SS.seek 25.00ml @08:16:14.41 Stroke completed -- waiting for pressure to equalize ... @08:16:15.05 Intake.select :close @08:16:15.18 SSV.dial :exhaust @08:16:15.95 Exhaust.select :open @08:16:16.05 SS.reconfigure SSexhale @08:16:16.09 SS.seek 0.00ml @08:16:25.37 Exhaust.select :close @08:16:25.46 SSV.dial CBV @08:16:25.48
CBV.dial SSV @08:16:26.25 Allowing 185s for 25.0ml of remaining 950.0ml @08:16:26.26 Intake.select :open @08:16:28.39 Intake @47.0psia, vacuum from 20.0 to 24.0psi @08:16:28.40 SS.reconfigure forSampling @08:16:28.44 SS.seek 25.00ml @08:16:47.59 Stroke completed -- waiting for pressure to equalize ... @08:16:48.22 Intake.select :close @08:16:48.36 SSV.dial :exhaust @08:16:49.11 Exhaust.select :open @08:16:49.20 SS.reconfigure SSexhale @08:16:49.24 SS.seek 0.00ml @08:16:58.31 Exhaust.select :close @08:16:58.40 SSV.dial CBV @08:16:58.42
CBV.dial SSV @08:16:59.18 Allowing 185s for 25.0ml of remaining 925.0ml @08:16:59.19 Intake.select :open @08:17:01.32 Intake @46.7psia, vacuum from 20.0 to 24.0psi @08:17:01.33 SS.reconfigure forSampling @08:17:01.37 SS.seek 25.00ml @08:17:09.26 Vacuum too high -- waiting up to 40s @08:17:09.53 Intake @42.3psia, vacuum from 20.0 to 24.0psi @08:17:09.54 SS.reconfigure forSampling @08:17:09.57 SS.seek 25.00ml @08:17:23.25 Stroke completed -- waiting for pressure to equalize ... @08:17:23.89 Intake.select :close @08:17:24.02 SSV.dial :exhaust @08:17:24.78 Exhaust.select :open @08:17:24.88 SS.reconfigure SSexhale @08:17:24.92 SS.seek 0.00ml @08:17:34.03 Exhaust.select :close @08:17:34.12 SSV.dial CBV @08:17:34.14
CBV.dial SSV @08:17:34.90 Allowing 185s for 25.0ml of remaining 900.0ml @08:17:34.91 Intake.select :open @08:17:37.04 Intake @46.9psia, vacuum from 20.0 to 24.0psi @08:17:37.05 SS.reconfigure forSampling @08:17:37.09 SS.seek 25.00ml @08:17:47.85 Vacuum too high -- waiting up to 40s @08:17:48.12 Intake @44.1psia, vacuum from 20.0 to 24.0psi @08:17:48.13 SS.reconfigure forSampling @08:17:48.16 SS.seek 25.00ml @08:18:01.84 Stroke completed -- waiting for pressure to equalize ... @08:18:02.48 Intake.select :close @08:18:02.61 SSV.dial :exhaust @08:18:03.38 Exhaust.select :open @08:18:03.47 SS.reconfigure SSexhale @08:18:03.51 SS.seek 0.00ml @08:18:12.52 Exhaust.select :close @08:18:12.61 SSV.dial CBV @08:18:12.63
CBV.dial SSV @08:18:13.40 Allowing 185s for 25.0ml of remaining 875.0ml @08:18:13.41 Intake.select :open @08:18:15.54 Intake @46.9psia, vacuum from 20.0 to 24.0psi @08:18:16.03 SS.reconfigure forSampling @08:18:16.06 SS.seek 25.00ml @08:18:27.76 Vacuum too high -- waiting up to 40s @08:18:28.03 Intake @44.3psia, vacuum from 20.0 to 24.0psi @08:18:28.04 SS.reconfigure forSampling @08:18:28.08 SS.seek 25.00ml @08:18:45.46 Stroke completed -- waiting for pressure to equalize ... @08:18:46.10 Intake.select :close @08:18:46.24 SSV.dial :exhaust @08:18:46.99 Exhaust.select :open @08:18:47.09 SS.reconfigure SSexhale @08:18:47.13 SS.seek 0.00ml @08:18:56.19 Exhaust.select :close @08:18:56.28 SSV.dial CBV @08:18:56.30
CBV.dial SSV @08:18:57.07 Allowing 185s for 25.0ml of remaining 850.0ml @08:18:57.08 Intake.select :open @08:18:59.21 Intake @46.6psia, vacuum from 20.0 to 24.0psi @08:18:59.22 SS.reconfigure forSampling @08:18:59.26 SS.seek 25.00ml @08:19:17.28 Vacuum too high -- waiting up to 40s @08:19:17.55 Intake @45.2psia, vacuum from 20.0 to 24.0psi @08:19:17.56 SS.reconfigure forSampling @08:19:17.59 SS.seek 25.00ml @08:19:35.18 Stroke completed -- waiting for pressure to equalize ... @08:19:35.82 Intake.select :close @08:19:35.95 SSV.dial :exhaust @08:19:36.72 Exhaust.select :open @08:19:36.82 SS.reconfigure SSexhale @08:19:36.86 SS.seek 0.00ml @08:19:45.99 Exhaust.select :close @08:19:46.08 SSV.dial CBV @08:19:46.10
CBV.dial SSV @08:19:46.88 Allowing 185s for 25.0ml of remaining 825.0ml @08:19:46.89 Intake.select :open @08:19:49.02 Intake @46.6psia, vacuum from 20.0 to 24.0psi @08:19:49.03 SS.reconfigure forSampling @08:19:49.07 SS.seek 25.00ml @08:20:17.47 Vacuum too high -- waiting up to 40s @08:20:17.74 Intake @45.5psia, vacuum from 20.0 to 24.0psi @08:20:17.75 SS.reconfigure forSampling @08:20:17.78 SS.seek 25.00ml @08:20:35.14 Stroke completed -- waiting for pressure to equalize ... @08:20:35.78 Intake.select :close @08:20:35.91 SSV.dial :exhaust @08:20:36.67 Exhaust.select :open @08:20:36.77 SS.reconfigure SSexhale @08:20:36.81 SS.seek 0.00ml @08:20:45.72 Exhaust.select :close @08:20:45.81 SSV.dial CBV @08:20:45.83
CBV.dial SSV @08:20:46.60 Allowing 185s for 25.0ml of remaining 800.0ml @08:20:46.61 Intake.select :open @08:20:48.74 Intake @46.8psia, vacuum from 20.0 to 24.0psi @08:20:48.75 SS.reconfigure forSampling @08:20:48.79 SS.seek 25.00ml @08:21:48.12 Stroke completed -- waiting for pressure to equalize ... @08:21:48.76 Intake.select :close @08:21:48.89 SSV.dial :exhaust @08:21:49.64 Exhaust.select :open @08:21:49.73 SS.reconfigure SSexhale @08:21:49.77 SS.seek 0.00ml @08:21:58.90 Exhaust.select :close @08:21:58.99 SSV.dial CBV @08:21:59.01
CBV.dial SSV @08:21:59.78 Allowing 185s for 25.0ml of remaining 775.0ml @08:21:59.79 Intake.select :open @08:22:01.93 Intake @47.4psia, vacuum from 20.0 to 24.0psi @08:22:01.94 SS.reconfigure forSampling @08:22:01.98 SS.seek 25.00ml @08:22:24.09 Vacuum too high -- waiting up to 40s @08:22:24.36 Intake @46.0psia, vacuum from 20.0 to 24.0psi @08:22:24.37 SS.reconfigure forSampling @08:22:24.40 SS.seek 25.00ml @08:23:34.33 Stroke completed -- waiting for pressure to equalize ... @08:23:34.97 Intake.select :close @08:23:35.10 SSV.dial :exhaust @08:23:35.87 Exhaust.select :open @08:23:35.96 SS.reconfigure SSexhale @08:23:36.00 SS.seek 0.00ml @08:23:44.99 Exhaust.select :close @08:23:45.08 SSV.dial CBV @08:23:45.10
CBV.dial SSV @08:23:45.87 Allowing 185s for 25.0ml of remaining 750.0ml @08:23:45.88 Intake.select :open @08:23:48.01 Intake @46.7psia, vacuum from 20.0 to 24.0psi @08:23:48.02 SS.reconfigure forSampling @08:23:48.06 SS.seek 25.00ml @08:25:38.40 Stroke completed -- waiting for pressure to equalize ... @08:25:39.62 Intake.select :close @08:25:39.76 SSV.dial :exhaust @08:25:40.51 Exhaust.select :open @08:25:40.60 SS.reconfigure SSexhale @08:25:40.64 SS.seek 0.00ml @08:25:49.72 Exhaust.select :close @08:25:49.81 SSV.dial CBV @08:25:49.84
CBV.dial SSV @08:25:50.61 Allowing 185s for 25.0ml of remaining 725.0ml @08:25:50.62 Intake.select :open @08:25:52.75 Intake @46.0psia, vacuum from 20.0 to 24.0psi @08:25:52.76 SS.reconfigure forSampling @08:25:52.80 SS.seek 25.00ml @08:27:43.15 Stroke completed -- waiting for pressure to equalize ... @08:27:44.95 Intake.select :close @08:27:45.09 SSV.dial :exhaust @08:27:45.87 Exhaust.select :open @08:27:45.96 SS.reconfigure SSexhale @08:27:46.00 SS.seek 0.00ml @08:27:55.44 Exhaust.select :close @08:27:55.53 SSV.dial CBV @08:27:55.55
CBV.dial SSV @08:27:56.82 Allowing 185s for 25.0ml of remaining 700.0ml @08:27:56.83 Intake.select :open @08:27:58.97 Intake @46.4psia, vacuum from 20.0 to 24.0psi @08:27:58.98 SS.reconfigure forSampling @08:27:59.02 SS.seek 25.00ml @08:28:47.20 Vacuum too high -- waiting up to 40s @08:28:47.47 Intake @45.8psia, vacuum from 20.0 to 24.0psi @08:28:47.48 SS.reconfigure forSampling @08:28:47.52 SS.seek 25.00ml @08:28:58.65 Vacuum too high -- waiting up to 40s @08:28:58.91 Intake @45.2psia, vacuum from 20.0 to 24.0psi @08:28:58.92 SS.reconfigure forSampling @08:28:58.96 SS.seek 25.00ml @08:29:13.80 Vacuum too high -- waiting up to 40s @08:29:14.07 Intake @45.3psia, vacuum from 20.0 to 24.0psi @08:29:14.08 SS.reconfigure forSampling @08:29:14.12 SS.seek 25.00ml @08:29:18.08 Vacuum too high -- waiting up to 40s @08:29:18.35 Intake @45.2psia, vacuum from 20.0 to 24.0psi @08:29:18.36 SS.reconfigure forSampling @08:29:18.39 SS.seek 25.00ml @08:29:24.98 Vacuum too high -- waiting up to 40s @08:29:25.25 Intake @44.9psia, vacuum from 20.0 to 24.0psi @08:29:25.26 SS.reconfigure forSampling @08:29:25.29 SS.seek 25.00ml @08:29:36.98 Vacuum too high -- waiting up to 40s @08:29:37.25 Intake @45.1psia, vacuum from 20.0 to 24.0psi @08:29:37.26 SS.reconfigure forSampling @08:29:37.29 SS.seek 25.00ml @08:29:44.51 Vacuum too high -- waiting up to 40s @08:29:44.78 Intake @45.3psia, vacuum from 20.0 to 24.0psi @08:29:44.79 SS.reconfigure forSampling @08:29:44.83 SS.seek 25.00ml @08:29:48.61 Vacuum too high -- waiting up to 40s @08:29:48.88 Intake @45.3psia, vacuum from 20.0 to 24.0psi @08:29:48.89 SS.reconfigure forSampling @08:29:48.92 SS.seek 25.00ml @08:29:51.24 Vacuum too high -- waiting up to 40s @08:29:51.50 Intake @45.1psia, vacuum from 20.0 to 24.0psi @08:29:51.51 SS.reconfigure forSampling @08:29:51.54 SS.seek 25.00ml @08:29:53.30 Vacuum too high -- waiting up to 40s @08:29:53.56 Intake @45.2psia, vacuum from 20.0 to 24.0psi @08:29:53.57 SS.reconfigure forSampling @08:29:53.60 SS.seek 25.00ml @08:29:54.37 Stroke completed -- waiting for pressure to equalize ... @08:29:55.59 Intake.select :close @08:29:55.74 SSV.dial :exhaust @08:29:56.50 Exhaust.select :open @08:29:56.61 SS.reconfigure SSexhale @08:29:56.65 SS.seek 0.00ml @08:30:05.76 Exhaust.select :close @08:30:05.85 SSV.dial CBV @08:30:05.87
CBV.dial SSV @08:30:06.64 Allowing 185s for 25.0ml of remaining 675.0ml @08:30:06.65 Intake.select :open @08:30:08.78 Intake @45.9psia, vacuum from 20.0 to 24.0psi @08:30:08.79 SS.reconfigure forSampling @08:30:08.83 SS.seek 25.00ml @08:30:11.02 Vacuum too high -- waiting up to 40s @08:30:11.28 Intake @45.0psia, vacuum from 20.0 to 24.0psi @08:30:11.29 SS.reconfigure forSampling @08:30:11.32 SS.seek 25.00ml @08:30:13.33 Vacuum too high -- waiting up to 40s @08:30:13.59 Intake @44.4psia, vacuum from 20.0 to 24.0psi @08:30:13.60 SS.reconfigure forSampling @08:30:13.63 SS.seek 25.00ml @08:30:22.60 Vacuum too high -- waiting up to 40s @08:30:22.86 Intake @44.9psia, vacuum from 20.0 to 24.0psi @08:30:22.87 SS.reconfigure forSampling @08:30:22.91 SS.seek 25.00ml @08:30:24.71 Vacuum too high -- waiting up to 40s @08:30:24.97 Intake @44.7psia, vacuum from 20.0 to 24.0psi @08:30:24.98 SS.reconfigure forSampling @08:30:25.01 SS.seek 25.00ml @08:30:26.61 Vacuum too high -- waiting up to 40s @08:30:26.87 Intake @44.8psia, vacuum from 20.0 to 24.0psi @08:30:26.88 SS.reconfigure forSampling @08:30:26.91 SS.seek 25.00ml @08:30:30.70 Vacuum too high -- waiting up to 40s @08:30:30.97 Intake @45.0psia, vacuum from 20.0 to 24.0psi @08:30:30.98 SS.reconfigure forSampling @08:30:31.01 SS.seek 25.00ml @08:30:32.43 Vacuum too high -- waiting up to 40s @08:30:32.69 Intake @44.1psia, vacuum from 20.0 to 24.0psi @08:30:32.70 SS.reconfigure forSampling @08:30:32.74 SS.seek 25.00ml @08:30:34.49 Vacuum too high -- waiting up to 40s @08:30:34.75 Intake @44.2psia, vacuum from 20.0 to 24.0psi @08:30:34.76 SS.reconfigure forSampling @08:30:34.79 SS.seek 25.00ml @08:30:36.58 Vacuum too high -- waiting up to 40s @08:30:36.84 Intake @44.1psia, vacuum from 20.0 to 24.0psi @08:30:36.85 SS.reconfigure forSampling @08:30:36.88 SS.seek 25.00ml @08:30:38.61 Vacuum too high -- waiting up to 40s @08:30:38.87 Intake @44.8psia, vacuum from 20.0 to 24.0psi @08:30:38.88 SS.reconfigure forSampling @08:30:38.91 SS.seek 25.00ml @08:30:42.05 Vacuum too high -- waiting up to 40s @08:30:42.31 Intake @44.6psia, vacuum from 20.0 to 24.0psi @08:30:42.84 SS.reconfigure forSampling @08:30:42.88 SS.seek 25.00ml @08:30:44.61 Vacuum too high -- waiting up to 40s @08:30:44.87 Intake @44.1psia, vacuum from 20.0 to 24.0psi @08:30:44.89 SS.reconfigure forSampling @08:30:44.92 SS.seek 25.00ml @08:30:46.58 Vacuum too high -- waiting up to 40s @08:30:46.84 Intake @44.0psia, vacuum from 20.0 to 24.0psi @08:30:46.85 SS.reconfigure forSampling @08:30:46.89 SS.seek 25.00ml @08:30:48.27 Vacuum too high -- waiting up to 40s @08:30:48.53 Intake @44.2psia, vacuum from 20.0 to 24.0psi @08:30:48.54 SS.reconfigure forSampling @08:30:48.58 SS.seek 25.00ml @08:30:50.05 Vacuum too high -- waiting up to 40s @08:30:50.32 SS.seek 25.00ml @08:30:52.27 Vacuum too high -- waiting up to 40s @08:30:52.53 Intake @44.9psia, vacuum from 20.0 to 24.0psi @08:30:52.54 SS.reconfigure forSampling @08:30:52.58 SS.seek 25.00ml @08:30:53.80 Vacuum too high -- waiting up to 40s @08:30:54.06 Intake @45.0psia, vacuum from 20.0 to 24.0psi @08:30:54.07 SS.reconfigure forSampling @08:30:54.11 SS.seek 25.00ml @08:30:55.27 Vacuum too high -- waiting up to 40s @08:30:55.53 Intake @44.1psia, vacuum from 20.0 to 24.0psi @08:30:55.55 SS.reconfigure forSampling @08:30:55.58 SS.seek 25.00ml @08:30:57.11 Vacuum too high -- waiting up to 40s @08:30:57.37 Intake @44.7psia, vacuum from 20.0 to 24.0psi @08:30:57.38 SS.reconfigure forSampling @08:30:57.42 SS.seek 25.00ml @08:30:58.61 Vacuum too high -- waiting up to 40s @08:30:58.87 Intake @44.4psia, vacuum from 20.0 to 24.0psi @08:30:58.88 SS.reconfigure forSampling @08:30:58.92 SS.seek 25.00ml @08:31:00.21 Vacuum too high -- waiting up to 40s @08:31:00.47 Intake @44.2psia, vacuum from 20.0 to 24.0psi @08:31:00.48 SS.reconfigure forSampling @08:31:00.52 SS.seek 25.00ml @08:31:02.02 Vacuum too high -- waiting up to 40s @08:31:02.28 Intake @44.8psia, vacuum from 20.0 to 24.0psi @08:31:02.29 SS.reconfigure forSampling @08:31:02.32 SS.seek 25.00ml @08:31:03.55 Vacuum too high -- waiting up to 40s @08:31:03.81 Intake @45.1psia, vacuum from 20.0 to 24.0psi @08:31:03.82 SS.reconfigure forSampling @08:31:03.85 SS.seek 25.00ml @08:31:05.27 Vacuum too high -- waiting up to 40s @08:31:05.54 SS.seek 25.00ml @08:31:06.74 Vacuum too high -- waiting up to 40s @08:31:07.00 Intake @44.6psia, vacuum from 20.0 to 24.0psi @08:31:07.01 SS.reconfigure forSampling @08:31:07.04 SS.seek 25.00ml @08:31:08.24 Vacuum too high -- waiting up to 40s @08:31:08.50 Intake @44.2psia, vacuum from 20.0 to 24.0psi @08:31:08.51 SS.reconfigure forSampling @08:31:08.55 SS.seek 25.00ml @08:31:09.89 Vacuum too high -- waiting up to 40s @08:31:10.15 Intake @44.4psia, vacuum from 20.0 to 24.0psi @08:31:10.16 SS.reconfigure forSampling @08:31:10.20 SS.seek 25.00ml @08:31:11.33 Vacuum too high -- waiting up to 40s @08:31:11.59 Intake @44.0psia, vacuum from 20.0 to 24.0psi @08:31:11.60 SS.reconfigure forSampling @08:31:11.64 SS.seek 25.00ml @08:31:12.80 Vacuum too high -- waiting up to 40s @08:31:13.06 Intake @44.0psia, vacuum from 20.0 to 24.0psi @08:31:13.07 SS.reconfigure forSampling @08:31:13.10 SS.seek 25.00ml @08:31:14.42 Vacuum too high -- waiting up to 40s @08:31:14.68 Intake @44.6psia, vacuum from 20.0 to 24.0psi @08:31:14.69 SS.reconfigure forSampling @08:31:14.72 SS.seek 25.00ml @08:31:15.89 Vacuum too high -- waiting up to 40s @08:31:16.15 Intake @44.5psia, vacuum from 20.0 to 24.0psi @08:31:16.16 SS.reconfigure forSampling @08:31:16.19 SS.seek 25.00ml @08:31:17.27 Vacuum too high -- waiting up to 40s @08:31:17.53 Intake @44.0psia, vacuum from 20.0 to 24.0psi @08:31:17.54 SS.reconfigure forSampling @08:31:17.57 SS.seek 25.00ml @08:31:18.74 Vacuum too high -- waiting up to 40s @08:31:19.00 Intake @44.2psia, vacuum from 20.0 to 24.0psi @08:31:19.01 SS.reconfigure forSampling @08:31:19.04 SS.seek 25.00ml @08:31:20.33 Vacuum too high -- waiting up to 40s @08:31:20.59 Intake @44.6psia, vacuum from 20.0 to 24.0psi @08:31:20.60 SS.reconfigure forSampling @08:31:20.64 SS.seek 25.00ml @08:31:21.83 Vacuum too high -- waiting up to 40s @08:31:22.09 Intake @44.6psia, vacuum from 20.0 to 24.0psi @08:31:22.10 SS.reconfigure forSampling @08:31:22.13 SS.seek 25.00ml @08:31:23.17 Vacuum too high -- waiting up to 40s @08:31:23.43 Intake @44.4psia, vacuum from 20.0 to 24.0psi @08:31:23.99 SS.reconfigure forSampling @08:31:24.02 SS.seek 25.00ml @08:31:25.86 Vacuum too high -- waiting up to 40s @08:31:26.12 Intake @44.7psia, vacuum from 20.0 to 24.0psi @08:31:26.13 SS.reconfigure forSampling @08:31:26.17 SS.seek 25.00ml @08:31:27.36 Vacuum too high -- waiting up to 40s @08:31:27.63 Intake @44.8psia, vacuum from 20.0 to 24.0psi @08:31:27.64 SS.reconfigure forSampling @08:31:27.67 SS.seek 25.00ml @08:31:28.70 Vacuum too high -- waiting up to 40s @08:31:28.97 Intake @44.4psia, vacuum from 20.0 to 24.0psi @08:31:28.98 SS.reconfigure forSampling @08:31:29.01 SS.seek 25.00ml @08:31:30.05 Vacuum too high -- waiting up to 40s @08:31:30.31 Intake @44.2psia, vacuum from 20.0 to 24.0psi @08:31:30.32 SS.reconfigure forSampling @08:31:30.36 SS.seek 25.00ml @08:31:31.55 Vacuum too high -- waiting up to 40s @08:31:31.82 SS.seek 25.00ml @08:31:33.02 Vacuum too high -- waiting up to 40s @08:31:33.28 Intake @44.6psia, vacuum from 20.0 to 24.0psi @08:31:33.29 SS.reconfigure forSampling @08:31:33.33 SS.seek 25.00ml @08:31:34.48 Vacuum too high -- waiting up to 40s @08:31:34.74 Intake @44.8psia, vacuum from 20.0 to 24.0psi @08:31:34.75 SS.reconfigure forSampling @08:31:34.79 SS.seek 25.00ml @08:31:35.86 Vacuum too high -- waiting up to 40s @08:31:36.12 Intake @44.9psia, vacuum from 20.0 to 24.0psi @08:31:36.13 SS.reconfigure forSampling @08:31:36.17 SS.seek 25.00ml @08:31:37.23 Vacuum too high -- waiting up to 40s @08:31:37.49 Intake @44.5psia, vacuum from 20.0 to 24.0psi @08:31:37.50 SS.reconfigure forSampling @08:31:37.53 SS.seek 25.00ml @08:31:38.58 Vacuum too high -- waiting up to 40s @08:31:38.84 Intake @44.5psia, vacuum from 20.0 to 24.0psi @08:31:38.85 SS.reconfigure forSampling @08:31:38.89 SS.seek 25.00ml @08:31:39.98 Vacuum too high -- waiting up to 40s @08:31:40.25 SS.seek 25.00ml @08:31:41.23 Vacuum too high -- waiting up to 40s @08:31:41.49 Intake @44.3psia, vacuum from 20.0 to 24.0psi @08:31:41.50 SS.reconfigure forSampling @08:31:41.54 SS.seek 25.00ml @08:31:42.64 Vacuum too high -- waiting up to 40s @08:31:42.90 Intake @44.1psia, vacuum from 20.0 to 24.0psi @08:31:42.91 SS.reconfigure forSampling @08:31:42.95 SS.seek 25.00ml @08:31:44.05 Vacuum too high -- waiting up to 40s @08:31:44.31 Intake @44.2psia, vacuum from 20.0 to 24.0psi @08:31:44.32 SS.reconfigure forSampling @08:31:44.35 SS.seek 25.00ml @08:31:45.30 Vacuum too high -- waiting up to 40s @08:31:45.56 Intake @44.9psia, vacuum from 20.0 to 24.0psi @08:31:45.57 SS.reconfigure forSampling @08:31:45.60 SS.seek 25.00ml @08:31:46.64 Vacuum too high -- waiting up to 40s @08:31:46.90 Intake @45.0psia, vacuum from 20.0 to 24.0psi @08:31:46.91 SS.reconfigure forSampling @08:31:46.95 SS.seek 25.00ml @08:31:48.08 Vacuum too high -- waiting up to 40s @08:31:48.34 Intake @44.6psia, vacuum from 20.0 to 24.0psi @08:31:48.35 SS.reconfigure forSampling @08:31:48.38 SS.seek 25.00ml @08:31:49.39 Vacuum too high -- waiting up to 40s @08:31:49.65 Intake @44.5psia, vacuum from 20.0 to 24.0psi @08:31:49.66 SS.reconfigure forSampling @08:31:49.69 SS.seek 25.00ml @08:31:50.64 Vacuum too high -- waiting up to 40s @08:31:50.90 Intake @44.6psia, vacuum from 20.0 to 24.0psi @08:31:50.91 SS.reconfigure forSampling @08:31:50.94 SS.seek 25.00ml @08:31:51.98 Vacuum too high -- waiting up to 40s @08:31:52.24 Intake @44.4psia, vacuum from 20.0 to 24.0psi @08:31:52.25 SS.reconfigure forSampling @08:31:52.28 SS.seek 25.00ml @08:31:53.36 Vacuum too high -- waiting up to 40s @08:31:53.62 Intake @44.6psia, vacuum from 20.0 to 24.0psi @08:31:53.63 SS.reconfigure forSampling @08:31:53.66 SS.seek 25.00ml @08:31:54.64 Vacuum too high -- waiting up to 40s @08:31:54.91 Intake @44.5psia, vacuum from 20.0 to 24.0psi @08:31:54.92 SS.reconfigure forSampling @08:31:54.96 SS.seek 25.00ml @08:31:55.89 Vacuum too high -- waiting up to 40s @08:31:56.15 Intake @44.5psia, vacuum from 20.0 to 24.0psi @08:31:56.16 SS.reconfigure forSampling @08:31:56.19 SS.seek 25.00ml @08:31:57.30 Vacuum too high -- waiting up to 40s @08:31:57.56 Intake @45.0psia, vacuum from 20.0 to 24.0psi @08:31:57.57 SS.reconfigure forSampling @08:31:57.60 SS.seek 25.00ml @08:31:58.64 Vacuum too high -- waiting up to 40s @08:31:58.90 Intake @44.9psia, vacuum from 20.0 to 24.0psi @08:31:59.46 SS.reconfigure forSampling @08:31:59.50 SS.seek 25.00ml @08:32:00.80 Vacuum too high -- waiting up to 40s @08:32:01.06 Intake @43.8psia, vacuum from 20.0 to 24.0psi @08:32:01.07 SS.reconfigure forSampling @08:32:01.11 SS.seek 25.00ml @08:32:02.08 Vacuum too high -- waiting up to 40s @08:32:02.35 SS.seek 25.00ml @08:32:03.39 Vacuum too high -- waiting up to 40s @08:32:03.87 Intake @43.9psia, vacuum from 20.0 to 24.0psi @08:32:03.88 SS.reconfigure forSampling @08:32:03.92 SS.seek 25.00ml @08:32:05.26 Vacuum too high -- waiting up to 40s @08:32:05.52 Intake @44.3psia, vacuum from 20.0 to 24.0psi @08:32:05.53 SS.reconfigure forSampling @08:32:05.57 SS.seek 25.00ml @08:32:06.58 Vacuum too high -- waiting up to 40s @08:32:07.06 Intake @44.6psia, vacuum from 20.0 to 24.0psi @08:32:07.07 SS.reconfigure forSampling @08:32:07.11 SS.seek 25.00ml @08:32:08.20 Vacuum too high -- waiting up to 40s @08:32:08.46 Intake @43.9psia, vacuum from 20.0 to 24.0psi @08:32:08.47 SS.reconfigure forSampling @08:32:08.51 SS.seek 25.00ml @08:32:09.61 Vacuum too high -- waiting up to 40s @08:32:09.88 Intake @43.8psia, vacuum from 20.0 to 24.0psi @08:32:09.89 SS.reconfigure forSampling @08:32:09.93 SS.seek 25.00ml @08:32:10.98 Vacuum too high -- waiting up to 40s @08:32:11.46 Intake @44.0psia, vacuum from 20.0 to 24.0psi @08:32:11.47 SS.reconfigure forSampling @08:32:11.50 SS.seek 25.00ml @08:32:12.51 Vacuum too high -- waiting up to 40s @08:32:12.99 Intake @44.1psia, vacuum from 20.0 to 24.0psi @08:32:13.00 SS.reconfigure forSampling @08:32:13.04 SS.seek 25.00ml @08:32:14.20 Vacuum too high -- waiting up to 40s @08:32:14.46 Intake @43.9psia, vacuum from 20.0 to 24.0psi @08:32:14.47 SS.reconfigure forSampling @08:32:14.50 SS.seek 25.00ml @08:32:15.58 Vacuum too high -- waiting up to 40s @08:32:15.84 Intake @43.5psia, vacuum from 20.0 to 24.0psi @08:32:15.85 SS.reconfigure forSampling @08:32:15.88 SS.seek 25.00ml @08:32:16.89 Vacuum too high -- waiting up to 40s @08:32:17.36 Intake @43.4psia, vacuum from 20.0 to 24.0psi @08:32:17.37 SS.reconfigure forSampling @08:32:17.41 SS.seek 25.00ml @08:32:18.58 Vacuum too high -- waiting up to 40s @08:32:19.06 Intake @43.7psia, vacuum from 20.0 to 24.0psi @08:32:19.07 SS.reconfigure forSampling @08:32:19.10 SS.seek 25.00ml @08:32:20.51 Vacuum too high -- waiting up to 40s @08:32:20.98 Intake @44.8psia, vacuum from 20.0 to 24.0psi @08:32:20.99 SS.reconfigure forSampling @08:32:21.02 SS.seek 25.00ml @08:32:22.13 Vacuum too high -- waiting up to 40s @08:32:22.40 Intake @44.2psia, vacuum from 20.0 to 24.0psi @08:32:22.41 SS.reconfigure forSampling @08:32:22.45 SS.seek 25.00ml @08:32:23.33 Vacuum too high -- waiting up to 40s @08:32:23.59 Intake @43.7psia, vacuum from 20.0 to 24.0psi @08:32:23.60 SS.reconfigure forSampling @08:32:23.63 SS.seek 25.00ml @08:32:24.54 Vacuum too high -- waiting up to 40s @08:32:24.80 Intake @43.3psia, vacuum from 20.0 to 24.0psi @08:32:24.81 SS.reconfigure forSampling @08:32:24.85 SS.seek 25.00ml @08:32:25.76 Vacuum too high -- waiting up to 40s @08:32:26.02 Intake @43.5psia, vacuum from 20.0 to 24.0psi @08:32:26.03 SS.reconfigure forSampling @08:32:26.06 SS.seek 25.00ml @08:32:27.14 Vacuum too high -- waiting up to 40s @08:32:27.61 Intake @43.8psia, vacuum from 20.0 to 24.0psi @08:32:27.62 SS.reconfigure forSampling @08:32:27.65 SS.seek 25.00ml @08:32:28.89 Vacuum too high -- waiting up to 40s @08:32:29.15 Intake @44.0psia, vacuum from 20.0 to 24.0psi @08:32:29.16 SS.reconfigure forSampling @08:32:29.19 SS.seek 25.00ml @08:32:30.11 Vacuum too high -- waiting up to 40s @08:32:30.58 Intake @44.3psia, vacuum from 20.0 to 24.0psi @08:32:30.59 SS.reconfigure forSampling @08:32:30.62 SS.seek 25.00ml @08:32:31.67 Vacuum too high -- waiting up to 40s @08:32:31.93 Intake @44.1psia, vacuum from 20.0 to 24.0psi @08:32:31.94 SS.reconfigure forSampling @08:32:31.97 SS.seek 25.00ml @08:32:33.01 Vacuum too high -- waiting up to 40s @08:32:33.84 Intake @44.0psia, vacuum from 20.0 to 24.0psi @08:32:33.85 SS.reconfigure forSampling @08:32:33.89 SS.seek 25.00ml @08:32:35.20 Vacuum too high -- waiting up to 40s @08:32:35.46 Intake @43.6psia, vacuum from 20.0 to 24.0psi @08:32:35.47 SS.reconfigure forSampling @08:32:35.51 SS.seek 25.00ml @08:32:36.39 Vacuum too high -- waiting up to 40s @08:32:36.87 Intake @43.8psia, vacuum from 20.0 to 24.0psi @08:32:36.88 SS.reconfigure forSampling @08:32:36.92 SS.seek 25.00ml @08:32:38.33 Vacuum too high -- waiting up to 40s @08:32:38.81 Intake @44.1psia, vacuum from 20.0 to 24.0psi @08:32:38.82 SS.reconfigure forSampling @08:32:38.86 SS.seek 25.00ml @08:32:40.20 Vacuum too high -- waiting up to 40s @08:32:40.67 Intake @44.1psia, vacuum from 20.0 to 24.0psi @08:32:40.68 SS.reconfigure forSampling @08:32:40.72 SS.seek 25.00ml @08:32:41.76 Stroke completed -- waiting for pressure to equalize ... @08:32:44.15 Intake.select :close @08:32:44.28 SSV.dial :exhaust @08:32:45.05 Exhaust.select :open @08:32:45.15 SS.reconfigure SSexhale @08:32:45.19 SS.seek 0.00ml @08:32:54.10 Exhaust.select :close @08:32:54.19 SSV.dial CBV @08:32:54.21
CBV.dial SSV @08:32:55.00 Allowing 185s for 25.0ml of remaining 650.0ml @08:32:55.01 Intake.select :open @08:32:57.14 Intake @44.3psia, vacuum from 20.0 to 24.0psi @08:32:57.15 SS.reconfigure forSampling @08:32:57.19 SS.seek 25.00ml @08:32:58.82 Vacuum too high -- waiting up to 40s @08:32:59.08 Intake @44.0psia, vacuum from 20.0 to 24.0psi @08:32:59.09 SS.reconfigure forSampling @08:32:59.12 SS.seek 25.00ml @08:32:59.98 Vacuum too high -- waiting up to 40s @08:33:00.24 Intake @44.0psia, vacuum from 20.0 to 24.0psi @08:33:00.25 SS.reconfigure forSampling @08:33:00.29 SS.seek 25.00ml @08:33:01.26 Vacuum too high -- waiting up to 40s @08:33:01.52 Intake @43.9psia, vacuum from 20.0 to 24.0psi @08:33:01.53 SS.reconfigure forSampling @08:33:01.56 SS.seek 25.00ml @08:33:02.45 Vacuum too high -- waiting up to 40s @08:33:02.71 Intake @43.8psia, vacuum from 20.0 to 24.0psi @08:33:02.72 SS.reconfigure forSampling @08:33:02.76 SS.seek 25.00ml @08:33:03.67 Vacuum too high -- waiting up to 40s @08:33:03.93 Intake @43.7psia, vacuum from 20.0 to 24.0psi @08:33:03.94 SS.reconfigure forSampling @08:33:03.98 SS.seek 25.00ml @08:33:04.89 Vacuum too high -- waiting up to 40s @08:33:05.15 Intake @43.4psia, vacuum from 20.0 to 24.0psi @08:33:05.16 SS.reconfigure forSampling @08:33:05.19 SS.seek 25.00ml @08:33:06.20 Vacuum too high -- waiting up to 40s @08:33:06.67 Intake @43.9psia, vacuum from 20.0 to 24.0psi @08:33:06.68 SS.reconfigure forSampling @08:33:06.72 SS.seek 25.00ml @08:33:07.70 Vacuum too high -- waiting up to 40s @08:33:07.96 Intake @43.6psia, vacuum from 20.0 to 24.0psi @08:33:07.97 SS.reconfigure forSampling @08:33:08.00 SS.seek 25.00ml @08:33:08.82 Vacuum too high -- waiting up to 40s @08:33:09.08 Intake @43.2psia, vacuum from 20.0 to 24.0psi @08:33:09.09 SS.reconfigure forSampling @08:33:09.12 SS.seek 25.00ml @08:33:09.92 Vacuum too high -- waiting up to 40s @08:33:10.39 Intake @43.2psia, vacuum from 20.0 to 24.0psi @08:33:10.40 SS.reconfigure forSampling @08:33:10.43 SS.seek 25.00ml @08:33:11.67 Vacuum too high -- waiting up to 40s @08:33:12.14 Intake @44.1psia, vacuum from 20.0 to 24.0psi @08:33:12.15 SS.reconfigure forSampling @08:33:12.18 SS.seek 25.00ml @08:33:13.17 Vacuum too high -- waiting up to 40s @08:33:13.43 Intake @43.9psia, vacuum from 20.0 to 24.0psi @08:33:13.44 SS.reconfigure forSampling @08:33:13.47 SS.seek 25.00ml @08:33:14.39 Vacuum too high -- waiting up to 40s @08:33:14.65 Intake @43.5psia, vacuum from 20.0 to 24.0psi @08:33:14.66 SS.reconfigure forSampling @08:33:14.69 SS.seek 25.00ml @08:33:15.60 Vacuum too high -- waiting up to 40s @08:33:16.08 Intake @43.9psia, vacuum from 20.0 to 24.0psi @08:33:16.09 SS.reconfigure forSampling @08:33:16.12 SS.seek 25.00ml @08:33:17.07 Vacuum too high -- waiting up to 40s @08:33:17.90 Intake @43.6psia, vacuum from 20.0 to 24.0psi @08:33:17.91 SS.reconfigure forSampling @08:33:17.95 SS.seek 25.00ml @08:33:19.35 Vacuum too high -- waiting up to 40s @08:33:19.62 SS.seek 25.00ml @08:33:20.42 Vacuum too high -- waiting up to 40s @08:33:20.90 Intake @44.2psia, vacuum from 20.0 to 24.0psi @08:33:20.91 SS.reconfigure forSampling @08:33:20.95 SS.seek 25.00ml @08:33:22.07 Vacuum too high -- waiting up to 40s @08:33:22.33 Intake @44.5psia, vacuum from 20.0 to 24.0psi @08:33:22.34 SS.reconfigure forSampling @08:33:22.38 SS.seek 25.00ml @08:33:23.26 Vacuum too high -- waiting up to 40s @08:33:23.52 Intake @44.2psia, vacuum from 20.0 to 24.0psi @08:33:23.53 SS.reconfigure forSampling @08:33:23.57 SS.seek 25.00ml @08:33:24.42 Vacuum too high -- waiting up to 40s @08:33:24.68 Intake @43.5psia, vacuum from 20.0 to 24.0psi @08:33:24.69 SS.reconfigure forSampling @08:33:24.73 SS.seek 25.00ml @08:33:25.70 Vacuum too high -- waiting up to 40s @08:33:25.96 Intake @43.9psia, vacuum from 20.0 to 24.0psi @08:33:25.97 SS.reconfigure forSampling @08:33:26.01 SS.seek 25.00ml @08:33:26.89 Vacuum too high -- waiting up to 40s @08:33:27.16 SS.seek 25.00ml @08:33:27.95 Vacuum too high -- waiting up to 40s @08:33:28.21 Intake @43.8psia, vacuum from 20.0 to 24.0psi @08:33:28.22 SS.reconfigure forSampling @08:33:28.26 SS.seek 25.00ml @08:33:29.07 Vacuum too high -- waiting up to 40s @08:33:29.33 Intake @43.6psia, vacuum from 20.0 to 24.0psi @08:33:29.34 SS.reconfigure forSampling @08:33:29.38 SS.seek 25.00ml @08:33:30.32 Vacuum too high -- waiting up to 40s @08:33:30.58 Intake @44.2psia, vacuum from 20.0 to 24.0psi @08:33:30.59 SS.reconfigure forSampling @08:33:30.63 SS.seek 25.00ml @08:33:31.51 Vacuum too high -- waiting up to 40s @08:33:31.98 Intake @44.4psia, vacuum from 20.0 to 24.0psi @08:33:31.99 SS.reconfigure forSampling @08:33:32.03 SS.seek 25.00ml @08:33:33.10 Vacuum too high -- waiting up to 40s @08:33:33.36 Intake @44.0psia, vacuum from 20.0 to 24.0psi @08:33:33.37 SS.reconfigure forSampling @08:33:33.40 SS.seek 25.00ml @08:33:34.23 Vacuum too high -- waiting up to 40s @08:33:34.49 Intake @43.6psia, vacuum from 20.0 to 24.0psi @08:33:34.50 SS.reconfigure forSampling @08:33:34.53 SS.seek 25.00ml @08:33:35.45 Vacuum too high -- waiting up to 40s @08:33:35.71 Intake @43.3psia, vacuum from 20.0 to 24.0psi @08:33:35.72 SS.reconfigure forSampling @08:33:35.75 SS.seek 25.00ml @08:33:36.67 Vacuum too high -- waiting up to 40s @08:33:37.14 Intake @43.6psia, vacuum from 20.0 to 24.0psi @08:33:37.15 SS.reconfigure forSampling @08:33:37.19 SS.seek 25.00ml @08:33:38.13 Vacuum too high -- waiting up to 40s @08:33:38.60 Intake @44.2psia, vacuum from 20.0 to 24.0psi @08:33:38.61 SS.reconfigure forSampling @08:33:38.64 SS.seek 25.00ml @08:33:39.79 Vacuum too high -- waiting up to 40s @08:33:40.26 Intake @44.7psia, vacuum from 20.0 to 24.0psi @08:33:40.27 SS.reconfigure forSampling @08:33:40.31 SS.seek 25.00ml @08:33:41.38 Vacuum too high -- waiting up to 40s @08:33:41.64 Intake @43.8psia, vacuum from 20.0 to 24.0psi @08:33:41.65 SS.reconfigure forSampling @08:33:41.69 SS.seek 25.00ml @08:33:42.54 Vacuum too high -- waiting up to 40s @08:33:42.80 Intake @43.5psia, vacuum from 20.0 to 24.0psi @08:33:42.81 SS.reconfigure forSampling @08:33:42.84 SS.seek 25.00ml @08:33:43.70 Vacuum too high -- waiting up to 40s @08:33:43.97 SS.seek 25.00ml @08:33:44.82 Vacuum too high -- waiting up to 40s @08:33:45.30 Intake @43.7psia, vacuum from 20.0 to 24.0psi @08:33:45.31 SS.reconfigure forSampling @08:33:45.34 SS.seek 25.00ml @08:33:46.35 Vacuum too high -- waiting up to 40s @08:33:46.61 Intake @43.5psia, vacuum from 20.0 to 24.0psi @08:33:46.62 SS.reconfigure forSampling @08:33:46.65 SS.seek 25.00ml @08:33:47.48 Vacuum too high -- waiting up to 40s @08:33:47.74 Intake @43.8psia, vacuum from 20.0 to 24.0psi @08:33:47.75 SS.reconfigure forSampling @08:33:47.78 SS.seek 25.00ml @08:33:49.27 Vacuum too high -- waiting up to 40s @08:33:49.54 Intake @44.3psia, vacuum from 20.0 to 24.0psi @08:33:49.55 SS.reconfigure forSampling @08:33:49.59 SS.seek 25.00ml @08:33:50.92 Vacuum too high -- waiting up to 40s @08:33:51.19 Intake @43.1psia, vacuum from 20.0 to 24.0psi @08:33:51.20 SS.reconfigure forSampling @08:33:51.23 SS.seek 25.00ml @08:33:52.13 Vacuum too high -- waiting up to 40s @08:33:52.62 Intake @43.3psia, vacuum from 20.0 to 24.0psi @08:33:52.63 SS.reconfigure forSampling @08:33:52.67 SS.seek 25.00ml @08:33:53.76 Vacuum too high -- waiting up to 40s @08:33:54.24 Intake @44.5psia, vacuum from 20.0 to 24.0psi @08:33:54.25 SS.reconfigure forSampling @08:33:54.29 SS.seek 25.00ml @08:33:55.13 Vacuum too high -- waiting up to 40s @08:33:55.39 Intake @42.7psia, vacuum from 20.0 to 24.0psi @08:33:55.40 SS.reconfigure forSampling @08:33:55.44 SS.seek 25.00ml @08:33:56.35 Vacuum too high -- waiting up to 40s @08:33:56.83 Intake @42.9psia, vacuum from 20.0 to 24.0psi @08:33:56.84 SS.reconfigure forSampling @08:33:56.88 SS.seek 25.00ml @08:33:57.92 Vacuum too high -- waiting up to 40s @08:33:58.40 Intake @43.1psia, vacuum from 20.0 to 24.0psi @08:33:58.41 SS.reconfigure forSampling @08:33:58.44 SS.seek 25.00ml @08:33:59.51 Vacuum too high -- waiting up to 40s @08:33:59.99 Intake @43.5psia, vacuum from 20.0 to 24.0psi @08:34:00.00 SS.reconfigure forSampling @08:34:00.04 SS.seek 25.00ml @08:34:01.10 Vacuum too high -- waiting up to 40s @08:34:01.58 Intake @43.3psia, vacuum from 20.0 to 24.0psi @08:34:01.59 SS.reconfigure forSampling @08:34:01.62 SS.seek 25.00ml @08:34:02.70 Vacuum too high -- waiting up to 40s @08:34:03.18 Intake @43.0psia, vacuum from 20.0 to 24.0psi @08:34:03.19 SS.reconfigure forSampling @08:34:03.22 SS.seek 25.00ml @08:34:04.29 Vacuum too high -- waiting up to 40s @08:34:04.77 Intake @42.3psia, vacuum from 20.0 to 24.0psi @08:34:04.78 SS.reconfigure forSampling @08:34:04.81 SS.seek 25.00ml @08:34:06.01 Vacuum too high -- waiting up to 40s @08:34:06.49 Intake @42.7psia, vacuum from 20.0 to 24.0psi @08:34:06.50 SS.reconfigure forSampling @08:34:06.53 SS.seek 25.00ml @08:34:07.63 Vacuum too high -- waiting up to 40s @08:34:08.10 Intake @42.8psia, vacuum from 20.0 to 24.0psi @08:34:08.11 SS.reconfigure forSampling @08:34:08.14 SS.seek 25.00ml @08:34:09.20 Vacuum too high -- waiting up to 40s @08:34:09.68 Intake @42.6psia, vacuum from 20.0 to 24.0psi @08:34:09.69 SS.reconfigure forSampling @08:34:09.72 SS.seek 25.00ml @08:34:10.79 Vacuum too high -- waiting up to 40s @08:34:11.27 Intake @43.2psia, vacuum from 20.0 to 24.0psi @08:34:11.28 SS.reconfigure forSampling @08:34:11.31 SS.seek 25.00ml @08:34:12.32 Vacuum too high -- waiting up to 40s @08:34:12.79 Intake @43.0psia, vacuum from 20.0 to 24.0psi @08:34:12.80 SS.reconfigure forSampling @08:34:12.83 SS.seek 25.00ml @08:34:13.91 Vacuum too high -- waiting up to 40s @08:34:14.39 Intake @42.5psia, vacuum from 20.0 to 24.0psi @08:34:14.40 SS.reconfigure forSampling @08:34:14.43 SS.seek 25.00ml @08:34:15.54 Vacuum too high -- waiting up to 40s @08:34:16.02 Intake @42.4psia, vacuum from 20.0 to 24.0psi @08:34:16.03 SS.reconfigure forSampling @08:34:16.06 SS.seek 25.00ml @08:34:17.13 Vacuum too high -- waiting up to 40s @08:34:17.61 Intake @42.3psia, vacuum from 20.0 to 24.0psi @08:34:17.62 SS.reconfigure forSampling @08:34:17.65 SS.seek 25.00ml @08:34:18.70 Vacuum too high -- waiting up to 40s @08:34:19.17 Intake @42.1psia, vacuum from 20.0 to 24.0psi @08:34:19.18 SS.reconfigure forSampling @08:34:19.22 SS.seek 25.00ml @08:34:20.20 Vacuum too high -- waiting up to 40s @08:34:21.26 Intake @42.6psia, vacuum from 20.0 to 24.0psi @08:34:21.27 SS.reconfigure forSampling @08:34:21.30 SS.seek 25.00ml @08:34:22.76 Vacuum too high -- waiting up to 40s @08:34:23.24 Intake @42.2psia, vacuum from 20.0 to 24.0psi @08:34:23.25 SS.reconfigure forSampling @08:34:23.29 SS.seek 25.00ml @08:34:24.41 Vacuum too high -- waiting up to 40s @08:34:24.90 SS.seek 25.00ml @08:34:25.88 Vacuum too high -- waiting up to 40s @08:34:26.36 Intake @42.6psia, vacuum from 20.0 to 24.0psi @08:34:26.37 SS.reconfigure forSampling @08:34:26.41 SS.seek 25.00ml @08:34:27.41 Vacuum too high -- waiting up to 40s @08:34:27.89 Intake @42.8psia, vacuum from 20.0 to 24.0psi @08:34:27.90 SS.reconfigure forSampling @08:34:27.93 SS.seek 25.00ml @08:34:28.82 Vacuum too high -- waiting up to 40s @08:34:29.30 Intake @41.8psia, vacuum from 20.0 to 24.0psi @08:34:29.31 SS.reconfigure forSampling @08:34:29.35 SS.seek 25.00ml @08:34:30.41 Vacuum too high -- waiting up to 40s @08:34:30.89 Intake @41.7psia, vacuum from 20.0 to 24.0psi @08:34:30.90 SS.reconfigure forSampling @08:34:30.93 SS.seek 25.00ml @08:34:32.07 Vacuum too high -- waiting up to 40s @08:34:32.55 Intake @41.8psia, vacuum from 20.0 to 24.0psi @08:34:32.56 SS.reconfigure forSampling @08:34:32.59 SS.seek 25.00ml @08:34:33.91 Vacuum too high -- waiting up to 40s @08:34:34.38 Intake @42.6psia, vacuum from 20.0 to 24.0psi @08:34:34.39 SS.reconfigure forSampling @08:34:34.42 SS.seek 25.00ml @08:34:35.23 Vacuum too high -- waiting up to 40s @08:34:35.70 Intake @42.7psia, vacuum from 20.0 to 24.0psi @08:34:35.71 SS.reconfigure forSampling @08:34:35.75 SS.seek 25.00ml @08:34:36.66 Vacuum too high -- waiting up to 40s @08:34:37.13 Intake @42.6psia, vacuum from 20.0 to 24.0psi @08:34:37.14 SS.reconfigure forSampling @08:34:37.18 SS.seek 25.00ml @08:34:38.19 Vacuum too high -- waiting up to 40s @08:34:38.67 Intake @42.3psia, vacuum from 20.0 to 24.0psi @08:34:38.68 SS.reconfigure forSampling @08:34:38.71 SS.seek 25.00ml @08:34:39.76 Vacuum too high -- waiting up to 40s @08:34:40.23 Intake @43.1psia, vacuum from 20.0 to 24.0psi @08:34:40.24 SS.reconfigure forSampling @08:34:40.27 SS.seek 25.00ml @08:34:41.10 Vacuum too high -- waiting up to 40s @08:34:41.57 Intake @43.3psia, vacuum from 20.0 to 24.0psi @08:34:41.58 SS.reconfigure forSampling @08:34:41.61 SS.seek 25.00ml @08:34:42.48 Vacuum too high -- waiting up to 40s @08:34:42.96 Intake @42.8psia, vacuum from 20.0 to 24.0psi @08:34:42.97 SS.reconfigure forSampling @08:34:43.00 SS.seek 25.00ml @08:34:43.94 Vacuum too high -- waiting up to 40s @08:34:44.41 Intake @42.0psia, vacuum from 20.0 to 24.0psi @08:34:44.42 SS.reconfigure forSampling @08:34:44.45 SS.seek 25.00ml @08:34:45.54 Vacuum too high -- waiting up to 40s @08:34:46.01 Intake @42.4psia, vacuum from 20.0 to 24.0psi @08:34:46.02 SS.reconfigure forSampling @08:34:46.06 SS.seek 25.00ml @08:34:47.10 Vacuum too high -- waiting up to 40s @08:34:47.58 Intake @42.5psia, vacuum from 20.0 to 24.0psi @08:34:47.59 SS.reconfigure forSampling @08:34:47.62 SS.seek 25.00ml @08:34:48.63 Vacuum too high -- waiting up to 40s @08:34:49.10 Intake @43.0psia, vacuum from 20.0 to 24.0psi @08:34:49.11 SS.reconfigure forSampling @08:34:49.14 SS.seek 25.00ml @08:34:50.01 Vacuum too high -- waiting up to 40s @08:34:50.48 Intake @43.0psia, vacuum from 20.0 to 24.0psi @08:34:50.49 SS.reconfigure forSampling @08:34:50.52 SS.seek 25.00ml @08:34:51.51 Vacuum too high -- waiting up to 40s @08:34:52.58 Intake @42.3psia, vacuum from 20.0 to 24.0psi @08:34:52.59 SS.reconfigure forSampling @08:34:52.63 SS.seek 25.00ml @08:34:54.10 Vacuum too high -- waiting up to 40s @08:34:54.58 Intake @42.2psia, vacuum from 20.0 to 24.0psi @08:34:54.59 SS.reconfigure forSampling @08:34:54.63 SS.seek 25.00ml @08:34:55.63 Vacuum too high -- waiting up to 40s @08:34:56.11 Intake @42.5psia, vacuum from 20.0 to 24.0psi @08:34:56.13 SS.reconfigure forSampling @08:34:56.16 SS.seek 25.00ml @08:34:57.10 Vacuum too high -- waiting up to 40s @08:34:57.58 Intake @42.8psia, vacuum from 20.0 to 24.0psi @08:34:57.59 SS.reconfigure forSampling @08:34:57.63 SS.seek 25.00ml @08:34:58.60 Vacuum too high -- waiting up to 40s @08:34:59.08 Intake @42.9psia, vacuum from 20.0 to 24.0psi @08:34:59.09 SS.reconfigure forSampling @08:34:59.13 SS.seek 25.00ml @08:35:00.19 Vacuum too high -- waiting up to 40s @08:35:00.67 Intake @42.5psia, vacuum from 20.0 to 24.0psi @08:35:00.68 SS.reconfigure forSampling @08:35:00.72 SS.seek 25.00ml @08:35:01.63 Vacuum too high -- waiting up to 40s @08:35:02.11 Intake @42.2psia, vacuum from 20.0 to 24.0psi @08:35:02.12 SS.reconfigure forSampling @08:35:02.16 SS.seek 25.00ml @08:35:03.10 Vacuum too high -- waiting up to 40s @08:35:03.58 Intake @41.9psia, vacuum from 20.0 to 24.0psi @08:35:03.59 SS.reconfigure forSampling @08:35:03.63 SS.seek 25.00ml @08:35:05.47 Vacuum too high -- waiting up to 40s @08:35:06.15 Intake @42.3psia, vacuum from 20.0 to 24.0psi @08:35:06.16 SS.reconfigure forSampling @08:35:06.19 SS.seek 25.00ml @08:35:07.13 Vacuum too high -- waiting up to 40s @08:35:07.61 Intake @42.5psia, vacuum from 20.0 to 24.0psi @08:35:07.62 SS.reconfigure forSampling @08:35:07.66 SS.seek 25.00ml @08:35:08.54 Vacuum too high -- waiting up to 40s @08:35:09.02 Intake @42.6psia, vacuum from 20.0 to 24.0psi @08:35:09.03 SS.reconfigure forSampling @08:35:09.06 SS.seek 25.00ml @08:35:10.07 Vacuum too high -- waiting up to 40s @08:35:10.66 Intake @42.6psia, vacuum from 20.0 to 24.0psi @08:35:10.67 SS.reconfigure forSampling @08:35:10.70 SS.seek 25.00ml @08:35:11.76 Vacuum too high -- waiting up to 40s @08:35:12.23 Intake @42.2psia, vacuum from 20.0 to 24.0psi @08:35:12.24 SS.reconfigure forSampling @08:35:12.28 SS.seek 25.00ml @08:35:13.60 Vacuum too high -- waiting up to 40s @08:35:14.08 Intake @41.9psia, vacuum from 20.0 to 24.0psi @08:35:14.09 SS.reconfigure forSampling @08:35:14.12 SS.seek 25.00ml @08:35:15.29 Vacuum too high -- waiting up to 40s @08:35:15.97 Intake @42.3psia, vacuum from 20.0 to 24.0psi @08:35:15.98 SS.reconfigure forSampling @08:35:16.01 SS.seek 25.00ml @08:35:17.01 Vacuum too high -- waiting up to 40s @08:35:17.48 Intake @42.4psia, vacuum from 20.0 to 24.0psi @08:35:17.49 SS.reconfigure forSampling @08:35:17.52 SS.seek 25.00ml @08:35:18.47 Vacuum too high -- waiting up to 40s @08:35:18.94 Intake @42.6psia, vacuum from 20.0 to 24.0psi @08:35:18.95 SS.reconfigure forSampling @08:35:18.98 SS.seek 25.00ml @08:35:20.00 Vacuum too high -- waiting up to 40s @08:35:20.47 Intake @42.2psia, vacuum from 20.0 to 24.0psi @08:35:20.48 SS.reconfigure forSampling @08:35:20.51 SS.seek 25.00ml @08:35:21.66 Vacuum too high -- waiting up to 40s @08:35:22.14 Intake @41.9psia, vacuum from 20.0 to 24.0psi @08:35:22.15 SS.reconfigure forSampling @08:35:22.18 SS.seek 25.00ml @08:35:23.50 Vacuum too high -- waiting up to 40s @08:35:24.78 Intake @42.3psia, vacuum from 20.0 to 24.0psi @08:35:24.79 SS.reconfigure forSampling @08:35:24.83 SS.seek 25.00ml @08:35:26.50 Vacuum too high -- waiting up to 40s @08:35:26.98 Intake @41.8psia, vacuum from 20.0 to 24.0psi @08:35:26.99 SS.reconfigure forSampling @08:35:27.03 SS.seek 25.00ml @08:35:28.25 Vacuum too high -- waiting up to 40s @08:35:28.96 Intake @42.1psia, vacuum from 20.0 to 24.0psi @08:35:28.97 SS.reconfigure forSampling @08:35:29.00 SS.seek 25.00ml @08:35:30.35 Vacuum too high -- waiting up to 40s @08:35:31.05 Intake @41.9psia, vacuum from 20.0 to 24.0psi @08:35:31.06 SS.reconfigure forSampling @08:35:31.10 SS.seek 25.00ml @08:35:32.72 Vacuum too high -- waiting up to 40s @08:35:33.42 SS.seek 25.00ml @08:35:34.72 Vacuum too high -- waiting up to 40s @08:35:35.40 Intake @41.4psia, vacuum from 20.0 to 24.0psi @08:35:35.41 SS.reconfigure forSampling @08:35:35.44 SS.seek 25.00ml @08:35:37.16 Vacuum too high -- waiting up to 40s @08:35:37.85 Intake @42.0psia, vacuum from 20.0 to 24.0psi @08:35:37.86 SS.reconfigure forSampling @08:35:37.89 SS.seek 25.00ml @08:35:39.19 Vacuum too high -- waiting up to 40s @08:35:39.66 Intake @42.2psia, vacuum from 20.0 to 24.0psi @08:35:39.67 SS.reconfigure forSampling @08:35:39.70 SS.seek 25.00ml @08:35:40.75 Vacuum too high -- waiting up to 40s @08:35:41.23 Intake @42.1psia, vacuum from 20.0 to 24.0psi @08:35:41.24 SS.reconfigure forSampling @08:35:41.27 SS.seek 25.00ml @08:35:42.44 Vacuum too high -- waiting up to 40s @08:35:43.12 Intake @42.0psia, vacuum from 20.0 to 24.0psi @08:35:43.13 SS.reconfigure forSampling @08:35:43.16 SS.seek 25.00ml @08:35:44.47 Vacuum too high -- waiting up to 40s @08:35:44.94 Intake @41.1psia, vacuum from 20.0 to 24.0psi @08:35:44.95 SS.reconfigure forSampling @08:35:44.99 SS.seek 25.00ml @08:35:46.00 Vacuum too high -- waiting up to 40s @08:35:46.47 Intake @40.9psia, vacuum from 20.0 to 24.0psi @08:35:46.48 SS.reconfigure forSampling @08:35:46.51 SS.seek 25.00ml @08:35:47.69 Vacuum too high -- waiting up to 40s @08:35:48.38 Intake @41.2psia, vacuum from 20.0 to 24.0psi @08:35:48.39 SS.reconfigure forSampling @08:35:48.42 SS.seek 25.00ml @08:35:49.82 Vacuum too high -- waiting up to 40s @08:35:50.51 Intake @41.7psia, vacuum from 20.0 to 24.0psi @08:35:50.52 SS.reconfigure forSampling @08:35:50.55 SS.seek 25.00ml @08:35:51.91 Vacuum too high -- waiting up to 40s @08:35:52.39 Intake @41.7psia, vacuum from 20.0 to 24.0psi @08:35:52.40 SS.reconfigure forSampling @08:35:52.44 SS.seek 25.00ml @08:35:53.60 Vacuum too high -- waiting up to 40s @08:35:54.08 Intake @40.9psia, vacuum from 20.0 to 24.0psi @08:35:54.09 SS.reconfigure forSampling @08:35:54.12 SS.seek 25.00ml @08:35:55.32 Vacuum too high -- waiting up to 40s @08:35:56.01 Intake @41.2psia, vacuum from 20.0 to 24.0psi @08:35:56.02 SS.reconfigure forSampling @08:35:56.05 SS.seek 25.00ml @08:35:57.47 Vacuum too high -- waiting up to 40s @08:35:58.17 Intake @41.9psia, vacuum from 20.0 to 24.0psi @08:35:58.18 SS.reconfigure forSampling @08:35:58.21 SS.seek 25.00ml @08:35:59.50 Vacuum too high -- waiting up to 40s @08:36:00.57 Intake @42.1psia, vacuum from 20.0 to 24.0psi @08:36:00.58 SS.reconfigure forSampling @08:36:00.61 SS.reconfigure SSconfig @08:36:00.65 SS.hold @08:36:00.74 Intake.select :close @08:36:00.89 ShallowSampler::Clogged in MAIN -- progress too slow after 374.0ml SSV.dial :exhaust @08:36:01.65 Exhaust.select :open @08:36:01.75 SS.reconfigure SSexhale @08:36:01.79 SS.seek 0.00ml @08:36:10.17 Exhaust.select :close @08:36:10.27 Sampled 374.0ml @08:36:10.28 collectSample: Complete Evacuating Puck (bubblePt=55.0psi, delay=15.0s) @08:36:10.29 Intake.select :close @08:36:10.35 CSV.dial :waste @08:36:10.37 CS.seek 0.000ml @08:36:10.48 SSV.dial CBV,avoiding => exhaust @08:36:10.50
CBV.dial SSV @08:36:10.54 CSV.dial :air @08:36:11.35 CS.seek 10.000ml @08:36:12.40
CTV.dial :air @08:36:13.18 delay 15 seconds @08:36:13.26 Sleepy.power :sampler,:OFF @08:36:28.18
SSV.dial :spit,avoiding => exhaust @08:36:29.52 CTV.dial CSV,avoiding => intake @08:36:29.54
SS.seek 0.00ml @08:36:29.64 SSV.dial CBV,avoiding => exhaust @08:36:35.48 CBV.dial between CSV and waste2b @08:36:36.42 CTV.dial CSV,avoiding => air @08:36:36.44
CSV.dial CTV @08:36:36.90 CS.reconfigure CSpressurize @08:36:36.93 CS.seek 0.000ml @08:37:01.17 BubblePoint: Intake @70.0psia, Outlet @14.7psia @08:37:01.18 SS.reconfigure SSconfig @08:37:01.21 SS.seek 10.00ml @08:37:06.96 delay 15 seconds @08:37:22.06 Evacuated: Intake @68.9psia, Outlet @-0.2psia @08:37:22.07 SSV.dial :spit,avoiding => exhaust @08:37:23.39 SS.reconfigure SSexhale @08:37:23.42 SS.seek 0.00ml @08:37:28.12 SS.reconfigure SSfast @08:37:28.15 SS.seek 5.00ml @08:37:30.94 SSV.dial CBV,avoiding => exhaust @08:37:30.96
CBV.dial SSV @08:37:32.28 CTV.dial :air,avoiding => intake @08:37:33.59 CSV.dial CTV @08:37:33.61
SS.reconfigure SSconfig @08:37:33.65 SS.seek 10.00ml @08:37:34.61 CSV.dial :air @08:37:35.13 CS.reconfigure CSfast @08:37:35.16 CS.seek 0.000ml @08:37:36.59
SSV.dial :spit,avoiding => exhaust @08:37:37.46 CS.coast @08:37:37.92
SS.reconfigure SSexhale @08:37:37.95 SS.seek 0.00ml @08:37:42.87 SS.reconfigure SSfast @08:37:42.90 SS.seek 5.00ml @08:37:45.74 SSV.dial CBV,avoiding => exhaust @08:37:45.76
CBV.dial SSV @08:37:48.07 SSV.dial :spit,avoiding => exhaust @08:37:49.38 SS.reconfigure SSconfig @08:37:49.41 SS.seek 0.00ml @08:37:52.52 SS.coast @08:37:52.55 Evacuating Puck (bubblePt=55.0psi, delay=15.0s) Intake.select :close @08:37:52.61 CSV.dial :waste @08:37:52.70 SSV.dial CBV,avoiding => exhaust @08:37:52.72
CBV.dial SSV @08:37:53.38 CS.seek 0.000ml @08:37:53.43 CSV.dial :air @08:37:54.05
CTV.dial :air @08:37:54.06 delay 15 seconds @08:37:54.63 CS.seek 10.000ml @08:38:09.06
SSV.dial :spit,avoiding => exhaust @08:38:10.39 CTV.dial CSV,avoiding => intake @08:38:10.41
SS.reconfigure SSexhale @08:38:10.48 SS.seek 0.00ml @08:38:10.83 SSV.dial CBV,avoiding => exhaust @08:38:18.26 CBV.dial between CSV and waste2b @08:38:19.20 CSV.dial between air and CTV @08:38:19.50 CS.seek 7.132ml @08:38:26.88 CTV.dial CSV,avoiding => air @08:38:26.89
CSV.dial CTV @08:38:27.41 CS.reconfigure CSpressurize @08:38:27.44 CS.seek 0.000ml @08:38:46.05 CSV.dial :air @08:38:46.48 CS.reconfigure CSfast @08:38:46.51 CS.seek 10.000ml @08:39:10.06 CSV.dial between air and CTV @08:39:10.45 CS.seek 2.131ml @08:39:29.09 CTV.dial CSV,avoiding => air @08:39:29.10
CSV.dial CTV @08:39:29.39 CS.reconfigure CSpressurize @08:39:29.42 CS.seek 0.000ml @08:39:31.19 BubblePoint: Intake @73.5psia, Outlet @17.9psia @08:39:31.71 SS.reconfigure SSconfig @08:39:31.74 SS.seek 10.00ml @08:39:37.58 delay 15 seconds @08:39:52.68 Evacuated: Intake @72.7psia, Outlet @-0.1psia SSV.dial :spit,avoiding => exhaust @08:39:54.00 SS.reconfigure SSexhale @08:39:54.03 SS.seek 0.00ml @08:39:59.44 SS.reconfigure SSfast @08:39:59.48 SS.seek 5.00ml @08:40:02.03 SSV.dial CBV,avoiding => exhaust @08:40:02.05
CBV.dial SSV @08:40:03.39 CTV.dial :air,avoiding => intake @08:40:04.70 CSV.dial CTV @08:40:04.71
SS.reconfigure SSconfig @08:40:04.75 SS.seek 10.00ml @08:40:05.71 CSV.dial :air @08:40:06.23 CS.reconfigure CSfast @08:40:06.26 CS.seek 0.000ml @08:40:07.91
SSV.dial :spit,avoiding => exhaust @08:40:09.25 SS.reconfigure SSexhale @08:40:09.28 SS.seek 0.00ml @08:40:10.86 CS.coast @08:40:14.14
SS.reconfigure SSfast @08:40:14.18 SS.seek 5.00ml @08:40:16.85 SSV.dial CBV,avoiding => exhaust @08:40:16.87
CBV.dial SSV @08:40:19.19 SSV.dial :spit,avoiding => exhaust @08:40:20.51 SS.reconfigure SSconfig @08:40:20.54 SS.seek 0.00ml @08:40:23.69 SS.coast @08:40:23.72 Lysing: Adding 1.5ml lysis CBV.dial between CSV and waste1,avoiding => DBV @08:40:25.20 CTV.dial CSV @08:40:26.47 CSV.dial CSR @08:40:27.25 CSR.select :lysis @08:40:27.35 CS.reconfigure CSconfig @08:40:27.39 CS.seek 1.000ml @08:40:30.71 delay 5 seconds @08:40:35.73 CSR.select :air @08:40:35.87 CS.seek 6.000ml @08:40:49.30 delay 2 seconds @08:40:51.32 CSV.dial :waste1 @08:40:52.59 CS.reconfigure CSfast @08:40:52.62 CS.seek empty @08:41:07.05 CSV.dial CSR @08:41:08.31 CSR.select :lysis @08:41:08.41 CS.reconfigure CSconfig @08:41:08.45 CS.seek 1.500ml @08:41:13.14 delay 5 seconds @08:41:18.16 CSR.select :air @08:41:18.30 CS.seek 4.500ml @08:41:26.70 delay 2 seconds @08:41:28.72 CSV.dial CTV @08:41:29.23 CS.seek 1.275ml @08:41:38.31 Heating sh1 puck from tube 2 in Collection Clamp to 85.0C for 5:00 @08:41:38.32 CH.seek 85.0C,8:00 @08:44:26.45 CH.hold 5:00,85.0C @08:49:26.56 Collection Puck Heater at 85.0C after lysing @08:49:26.57 CS.seek 0.000ml @08:49:30.61 CTV.dial between spare and air,avoiding => intake @08:49:31.60 CSV.dial :air @08:49:32.05 CS.seek 5.000ml @08:49:45.50 CSV.dial CTV @08:49:46.02 delay 2 seconds @08:49:48.03 CSV.dial :air @08:49:48.55 delay 2 seconds @08:49:50.56 CSV.dial CTV @08:49:51.08 delay 2 seconds @08:49:53.09 CSV.dial :air @08:49:53.60 delay 2 seconds @08:49:55.61 CS.seek 0.000ml @08:50:09.07 Recovering Lysate into Collection syringe @08:50:09.08 CSV.dial CBV @08:50:10.07 CBV.dial CSV @08:50:10.43 CS.seek 5.000ml @08:50:23.85 delay 5 seconds @08:50:28.87 CTV.dial :air @08:50:29.35 CS.seek 7.000ml @08:50:35.28 delay 5 seconds @08:50:40.30 CSV.dial :air2 @08:50:40.73 CS.seek 10.000ml @08:50:49.13 CSV.dial between air and CTV,avoiding => CBV @08:50:50.28 Lysing Complete @08:50:50.33 Storing sh1 puck in Collection Clamp to tube 1 @08:50:50.45 SC.reconfigure SCtight @08:50:50.48 SC.seek 1 @08:50:52.69 SC.reconfigure SCloose @08:50:52.86 Elbow.reconfigure TightElbow @08:50:52.89 Elbow.seek 1 @08:50:54.51 Forearm.reconfigure TightForearm @08:50:54.54 Forearm.seek 1 + 800 counts @08:50:55.67 SE.reconfigure SEpick @08:50:55.72 SE.seek beamBreak @08:50:58.19 SE.configure SEconfig @08:50:58.31 Elbow.reconfigure LimpElbow @08:50:58.34 Forearm.reconfigure LooseForearm @08:50:58.38 Forearm.seek 1 @08:50:58.61 Elbow.hold @08:50:58.66 Elbow.reconfigure TightElbow @08:50:58.74 SE.seek 5.04 Pucks below Top Plate @08:50:59.05 Forearm.coast @08:50:59.08 Forearm.seek 1 + 326 counts @08:50:59.32 Forearm.reconfigure TightForearm @08:50:59.35 Forearm.seek 1 + 800 counts @08:50:59.54 SE.seek 6.04 Pucks below Top Plate @08:51:00.10 Forearm.reconfigure LooseForearm @08:51:00.14 Forearm.seek retract @08:51:01.06 Elbow.seek CC @08:51:03.36 Elbow.reconfigure LooseElbow @08:51:03.44 Forearm.seek CC @08:51:04.35 CC.seek sealed @08:51:05.28 Hand.grip @08:51:05.99 CC.seek open @08:51:09.33 Forearm.seek retract @08:51:10.27 Elbow.reconfigure TightElbow @08:51:10.30 Elbow.seek 1 @08:51:11.92 Elbow.reconfigure LimpElbow @08:51:11.97 Forearm.seek 1 @08:51:13.00 Elbow.hold @08:51:13.05 Elbow.reconfigure TightElbow @08:51:13.09 Hand.release @08:51:13.95 Forearm.coast @08:51:13.98 Forearm.seek 1 + 308 counts @08:51:14.21 Forearm.reconfigure TightForearm @08:51:14.24 Forearm.seek 1 + 800 counts @08:51:14.61 SE.seek down @08:51:15.71
Forearm.reconfigure LooseForearm @08:51:15.75 Forearm.seek retract @08:51:16.72 SE.coast @08:51:16.75
SC.coast @08:51:16.78 Tranferring lysate to PRV CSV.dial :air @08:51:17.07 CS.seek 10.000ml @08:51:17.13 CSV.dial between air and CTV @08:51:18.53 AIV.dial :bypass @08:51:18.60 Beginning samplePassC @08:51:18.61 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:51:18.75 Elbow.seek FlushPuck::Garage @08:51:20.48 Elbow.reconfigure LooseElbow @08:51:20.57 Forearm.seek garage @08:51:21.56 Hand.grip @08:51:22.33 Forearm.seek retract @08:51:23.33 Elbow.reconfigure TightElbow @08:51:23.37 Elbow.seek CC @08:51:26.13 Elbow.reconfigure LooseElbow @08:51:26.22 Forearm.reconfigure TightForearm @08:51:26.25 Forearm.seek CC @08:51:27.30 CC.seek closed @08:51:31.05 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.95 @08:51:31.10 Hand.release @08:51:31.91 Forearm.reconfigure LooseForearm @08:51:31.94 Forearm.seek retract @08:51:32.87 AWV.select :air @08:51:32.88 AIV.dial :bypass @08:51:32.89 PSV.dial PSR @08:51:34.19 PSR.select :flush @08:51:34.29 PS.seek 1.500ml @08:51:50.33 delay 5 seconds @08:51:55.35 PSV.dial :waste1 @08:51:55.79 PS.seek 0.000ml @08:52:11.80 PSV.dial PSR @08:52:12.25 PSR.select :air @08:52:12.35 PS.seek 2.500ml @08:52:38.65 CBV.dial PRV @08:52:38.66 PRV.dial CBV @08:52:39.42
PSV.dial :mixloop @08:52:40.42 CTV.dial :air,avoiding => intake @08:52:40.43 PTV.dial :PRVmixing @08:52:40.51 PS.seek 0.000ml @08:53:06.70 PSV.dial :air @08:53:07.15 PS.seek 2.500ml @08:53:33.45 PSV.dial :mixloop @08:53:33.91 PS.seek 0.000ml @08:54:00.14 PSV.dial :air @08:54:00.60 PS.seek 2.500ml @08:54:26.83 PSV.dial :mixloop @08:54:27.27 PS.seek 0.000ml @08:54:53.51 Garaging FlushPuck in Collection Clamp @08:54:53.57 Elbow.reconfigure TightElbow @08:54:53.60 Elbow.seek CC @08:54:54.21 Elbow.reconfigure LooseElbow @08:54:54.29 Forearm.seek CC @08:54:55.27 CC.seek sealed @08:54:56.21 Hand.grip @08:54:56.98 CC.seek open @08:55:00.27 Forearm.seek retract @08:55:01.21 Elbow.reconfigure TightElbow @08:55:01.25 Elbow.seek garage @08:55:04.07 Elbow.reconfigure LooseElbow @08:55:04.15 Forearm.reconfigure TightForearm @08:55:04.18 Forearm.seek garage @08:55:05.33 Hand.release @08:55:06.15 Forearm.reconfigure LooseForearm @08:55:06.18 Forearm.seek retract @08:55:07.19 Connecting PS to CS to transfer sample @08:55:07.20 CBV.dial AIV @08:55:07.21 AIV.dial :bypass @08:55:07.22 PSV.dial :mixloop @08:55:07.23 PTV.dial :PRVmixing @08:55:07.24 PRV.dial AIV CTV.dial :air,avoiding => intake @08:55:07.25 Loading lyfil puck from tube 2 into Collection Clamp @08:55:07.42 SC.reconfigure SCtight @08:55:07.45 SC.seek 2 @08:55:09.71 SC.reconfigure SCloose @08:55:09.89 Elbow.reconfigure TightElbow @08:55:09.92 Elbow.seek 2 @08:55:11.77 Forearm.reconfigure TightForearm @08:55:11.80 Forearm.seek 2 + 800 counts @08:55:12.84 SE.reconfigure SEpick @08:55:12.89 SE.seek beamBreak @08:55:14.44 SE.configure SEconfig @08:55:14.56 Elbow.seek 2 @08:55:14.70 Forearm.seek 2 + 300 counts @08:55:15.07 Forearm.reconfigure LooseForearm @08:55:15.11 Hand.grip @08:55:15.86 SE.seek down @08:55:16.01
Forearm.seek retract @08:55:16.97 Elbow.seek CC @08:55:17.24 SE.coast @08:55:18.49
Elbow.reconfigure LooseElbow @08:55:18.57 Forearm.reconfigure TightForearm @08:55:18.60 Forearm.seek CC @08:55:19.68 CC.seek closed @08:55:23.49 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.96 @08:55:23.50 Hand.release @08:55:24.27 Forearm.reconfigure LooseForearm @08:55:24.30 Forearm.seek retract @08:55:25.27 SC.coast @08:55:25.30 CTV.dial CSV,avoiding => intake @08:55:26.60 CSV.dial CTV @08:55:26.97 CS.seek 5.000ml @08:55:40.46 delay 1:20 @08:57:00.47 CS.seek 2.500ml @08:57:07.64 delay 40 seconds @08:57:47.65 CS.seek 0.000ml @08:57:54.80 delay 1:00 @08:58:54.82 PSV.dial :air @08:58:55.27 PS.seek 2.500ml @08:59:21.48 PSV.dial :mixloop @08:59:21.92 delay 3 seconds @08:59:24.93 PSV.dial :air @08:59:25.37 delay 2 seconds @08:59:27.38 PSV.dial :mixloop @08:59:27.81 delay 3 seconds @08:59:30.82 PSV.dial :waste1 @08:59:32.12 PS.seek empty @08:59:58.32 CBV.dial :waste1 @08:59:58.78 delay 5 seconds @09:00:03.79 CBV.dial AIV @09:00:04.25 delay 10 seconds @09:00:14.26 CBV.dial :waste1 @09:00:14.71 delay 10 seconds @09:00:24.72 CBV.dial AIV @09:00:25.18 delay 10 seconds @09:00:35.19 CTV.dial CSV,avoiding => intake @09:00:35.20 CBV.dial between AIV and waste1 @09:00:35.53 CSV.dial :air @09:00:36.26 CS.seek 10.000ml @09:01:02.30 CSV.dial CTV @09:01:02.73 CS.seek 0.000ml @09:01:28.80 CBV.dial :waste1 @09:01:29.09 delay 10 seconds @09:01:39.10 CTV.dial :air,avoiding => intake @09:01:41.02 Storing lyfil puck in Collection Clamp to tube 1 @09:01:41.15 SC.reconfigure SCtight @09:01:41.18 SC.seek 1 @09:01:43.46 SC.reconfigure SCloose @09:01:43.64 Elbow.reconfigure TightElbow @09:01:43.67 Elbow.seek 1 @09:01:45.33 Forearm.reconfigure TightForearm @09:01:45.36 Forearm.seek 1 + 800 counts @09:01:46.50 SE.reconfigure SEpick @09:01:46.55 SE.seek beamBreak @09:01:48.92 SE.configure SEconfig @09:01:49.04 Elbow.reconfigure LimpElbow @09:01:49.07 Forearm.reconfigure LooseForearm @09:01:49.11 Forearm.seek 1 @09:01:49.33 Elbow.hold @09:01:49.38 Elbow.reconfigure TightElbow @09:01:49.45 SE.seek 6.05 Pucks below Top Plate @09:01:49.76 Forearm.coast @09:01:49.80 Forearm.seek 1 + 333 counts @09:01:50.04 Forearm.reconfigure TightForearm @09:01:50.07 Forearm.seek 1 + 800 counts @09:01:50.32 SE.seek 7.05 Pucks below Top Plate @09:01:50.87 Forearm.reconfigure LooseForearm @09:01:50.90 Forearm.seek retract @09:01:51.82 Elbow.seek CC @09:01:53.36 Elbow.reconfigure LooseElbow @09:01:53.44 Forearm.seek CC @09:01:54.40 CC.seek sealed @09:01:55.33 Hand.grip @09:01:56.10 CC.seek open @09:01:59.43 Forearm.seek retract @09:02:00.37 Elbow.reconfigure TightElbow @09:02:00.41 Elbow.seek 1 @09:02:02.01 Elbow.reconfigure LimpElbow @09:02:02.06 Forearm.seek 1 @09:02:03.01 Elbow.hold @09:02:03.06 Elbow.reconfigure TightElbow @09:02:03.10 Hand.release @09:02:03.93 Forearm.coast @09:02:03.96 Forearm.seek 1 + 334 counts @09:02:04.19 Forearm.reconfigure TightForearm @09:02:04.22 Forearm.seek 1 + 800 counts @09:02:04.48 SE.seek down @09:02:05.67
Forearm.reconfigure LooseForearm @09:02:05.71 Forearm.seek retract @09:02:06.50 SE.coast @09:02:06.62
SC.coast @09:02:06.65 PSV.dial :air @09:02:07.70 PS.seek 0.499ml @09:02:13.63 delay 3 seconds @09:02:16.64 PSV.dial :mixloop @09:02:17.07 PRV.dial :air @09:02:17.52 delay 3 seconds @09:02:20.53 PRV.dial CBV @09:02:20.97 CBV.dial AIV @09:02:21.42 PRV.dial between CBV and air @09:02:21.74 PRV.dial :air @09:02:22.09 PS.reconfigure PSslow @09:02:22.12 PS.seek 0.599ml @09:02:24.66 PRV.dial :waste2 @09:02:25.39 PS.reconfigure PSconfig @09:02:25.42 PS.seek empty @09:02:32.35 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @09:02:32.49 Elbow.seek FlushPuck::Garage @09:02:34.26 Elbow.reconfigure LooseElbow @09:02:34.35 Forearm.seek garage @09:02:35.34 Hand.grip @09:02:36.18 Forearm.seek retract @09:02:37.18 Elbow.reconfigure TightElbow @09:02:37.22 Elbow.seek CC @09:02:39.97 Elbow.reconfigure LooseElbow @09:02:40.06 Forearm.reconfigure TightForearm @09:02:40.09 Forearm.seek CC @09:02:41.81 CC.seek closed @09:02:45.64 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 2.02 @09:02:45.69 Hand.release @09:02:46.51 Forearm.reconfigure LooseForearm @09:02:46.54 Forearm.seek retract @09:02:47.53 Clearing PML for modified lysate @09:02:47.54 CTV.dial :air,avoiding => intake @09:02:47.55 PRV.dial :waste2 @09:02:47.56 PTV.dial :PRV,avoiding => puck @09:02:48.31 delay 2 seconds @09:02:50.31 PTV.dial :PRVmixing,avoiding => puck @09:02:51.05 delay 2 seconds @09:02:53.06 PSV.dial :PML @09:02:53.07 delay 2 seconds @09:02:55.07 PRV.dial :air @09:02:55.80 delay 2 seconds @09:02:57.86 PS.seek 1.499ml @09:03:13.94 delay 5 seconds @09:03:18.95 PS.seek 2.500ml @09:03:30.03 PSV.dial :air @09:03:30.51 PS.seek 0.000ml @09:03:56.78 mixing lysate with speDil @09:03:56.79 PSV.dial :PML @09:03:57.24 CBV.dial :PRV @09:03:57.25 PRV.dial :air @09:03:57.26 delay 2 seconds @09:03:59.27 PRV.dial :speDil @09:03:59.71 delay 2 seconds @09:04:01.77 PS.reconfigure PSslow1 @09:04:01.80 PS.seek 0.225ml @09:04:25.35 delay 5 seconds @09:04:30.36 PRV.dial CBV @09:04:31.08 delay 2 seconds @09:04:33.14 PS.seek 0.475ml @09:04:58.94 delay 5 seconds @09:05:03.95 PRV.dial :air @09:05:04.41 delay 2 seconds @09:05:06.42 PS.seek 1.000ml @09:06:00.09 delay 10 seconds @09:06:10.10 PRV.dial CBV @09:06:10.54 CTV.dial :air,avoiding => intake @09:06:10.55 PSV.dial :air @09:06:11.02 PS.reconfigure PSconfig @09:06:11.05 PS.seek 2.500ml @09:06:27.03 delay 3 seconds @09:06:30.04 PSV.dial PTV @09:06:30.78 PTV.dial :PRV,avoiding => puck @09:06:31.52 PS.seek 0.000ml @09:06:57.71 PSV.dial :air @09:06:58.46 PS.seek 2.500ml @09:07:24.68 delay 3 seconds @09:07:27.69 PSV.dial PTV @09:07:28.40 PTV.dial :PRV,avoiding => puck @09:07:28.42 PS.seek 0.000ml @09:07:54.71 Pushing modified lysate into AIV Injection Loop @09:07:54.72 CBV.dial AIV @09:07:54.73 AIV.dial :bypass @09:07:54.74 PRV.dial CBV @09:07:54.75 PSV.dial :air @09:07:55.47 PS.seek 2.500ml @09:08:21.81 delay 3 seconds @09:08:24.83 PSV.dial :PML @09:08:25.27 PTV.dial :PRVmixing,avoiding => puck @09:08:26.06 PS.reconfigure PSslow1 @09:08:26.09 PS.seek 1.740ml @09:09:43.71 delay 5 seconds @09:09:48.72 AIV.dial :sample @09:09:49.24 AWV.select :waste @09:09:49.40 PS.reconfigure PSslow2 @09:09:49.43 PS.seek 1.220ml @09:11:35.30 delay 15 seconds @09:11:50.31 AIV.dial :bypass @09:11:50.86 PS.reconfigure PSconfig @09:11:50.89 PS.seek 0.000ml @09:12:04.17 PSV.dial :air @09:12:04.62 PS.seek 2.500ml @09:12:30.83 PSV.dial :PML @09:12:31.28 PS.seek 0.000ml @09:12:57.51 AIV.dial :coilToMFB @09:12:57.52 AWV.select :air @09:12:57.66 Extracting Nucleic Acid from AIV @09:12:57.68
Beginning samplePassD @09:12:57.69 SPE.hold 2:46:40,55.0C @09:12:57.71 ASV.dial :SPEdelivery @09:12:57.84
AWV.select :air @09:12:57.85 AIV.dial :bypass PSV.dial PSR @09:12:58.54 delay 20 seconds @09:12:58.89
PSR.select :killMFB @09:12:58.99 PS.seek 1.500ml @09:13:15.11 delay 5 seconds @09:13:18.55 primeLysateINT @09:13:18.56 AWV.select :air @09:13:18.57 AIV.dial :coilToMFB ATV.dial :AIV @09:13:19.92 AS.reconfigure ASslow1 @09:13:19.95 AS.seek 0.241ml @09:13:20.12
PSV.dial :waste1 @09:13:20.57 PS.seek 0.000ml @09:13:36.70 PSV.dial PSR @09:13:37.72 PSR.select :air @09:13:37.82 PS.seek 2.500ml @09:14:04.11 PRV.dial CBV @09:14:04.13
CBV.dial PRV @09:14:04.14 PSV.dial :mixloop @09:14:05.17 CTV.dial :air,avoiding => intake @09:14:05.18 PTV.dial :PRVmixing @09:14:05.20 PS.seek 0.000ml @09:14:09.62 delay 10 seconds @09:14:19.63 ATV.dial :air2 @09:14:20.23 AS.seek 0.271ml @09:14:26.60 ATV.dial :waste2 @09:14:27.07 AS.reconfigure ASnorm @09:14:27.10 AS.seek empty @09:14:31.51
PSV.dial PSR @09:14:32.56 PSR.select :flush @09:14:32.66 PS.seek 1.500ml @09:14:41.37 Lysate primed! @09:14:41.38 AFV.select :holdingCoil @09:14:41.39 ATV.dial :air2 @09:14:41.78 AS.reconfigure ASslow1 @09:14:41.81 AS.seek 0.069ml @09:14:48.85
delay 5 seconds @09:14:53.86 PSV.dial :waste1 @09:14:54.30 PS.seek 0.000ml @09:14:56.43 delay 3 seconds @09:14:59.44 ATV.dial :AIV @09:15:00.04 AS.seek 0.469ml @09:15:10.38
PSV.dial PSR @09:15:10.84 PSR.select :air @09:15:10.94 PS.seek 2.500ml @09:15:37.38 PRV.dial CBV @09:15:37.40
CBV.dial PRV @09:15:37.41 PSV.dial :mixloop @09:15:38.54 CTV.dial :air,avoiding => intake @09:15:38.55 PTV.dial :PRVmixing @09:15:38.57 PS.seek 0.000ml @09:16:04.86 PSV.dial PSR @09:16:05.88 PSR.select :flush @09:16:05.98 PS.seek 1.500ml @09:16:21.89 delay 5 seconds @09:16:22.05
delay 5 seconds @09:16:26.94 Solid Phase Extraction Heater at 51.4C @09:16:26.95 ATV.dial ASV @09:16:27.06
PSV.dial :waste1 @09:16:27.28 ASV.dial :SPEdelivery @09:16:27.34 AS.seek 0.041ml @09:16:27.51
PS.seek 0.000ml @09:16:43.74 PSV.dial PSR @09:16:44.19 PSR.select :air @09:16:44.29 PS.seek 2.500ml @09:17:10.55 PRV.dial CBV @09:17:10.57
CBV.dial PRV @09:17:10.58 PSV.dial :mixloop @09:17:11.70 CTV.dial :air,avoiding => intake @09:17:11.71 PTV.dial :PRVmixing @09:17:11.73 PS.seek 0.000ml @09:17:38.17 PSV.dial PSR @09:17:39.17 PSR.select :flush @09:17:39.27 PS.seek 1.500ml @09:17:54.87 delay 10 seconds @09:17:55.39
delay 5 seconds @09:18:00.40 PSV.dial :waste1 @09:18:00.85 PS.seek 0.000ml @09:18:04.88 rinseLysateLineINT @09:18:04.89 AFV.select :carrierSolution @09:18:04.98 delay 2 seconds @09:18:06.99 AS.reconfigure ASconfig @09:18:07.03 AS.seek 1.000ml @09:18:16.89
PSV.dial PSR @09:18:17.34 PSR.select :air @09:18:17.44 PS.seek 2.500ml @09:18:43.70 PRV.dial CBV @09:18:43.72
CBV.dial PRV @09:18:43.73 PSV.dial :mixloop @09:18:44.73 CTV.dial :air,avoiding => intake @09:18:44.74 PTV.dial :PRVmixing @09:18:44.75 PS.reconfigure PSslow @09:18:44.79 PS.seek 1.200ml @09:19:03.20 delay 1 second @09:19:04.21 AFV.select :holdingCoil @09:19:04.30 delay 1 second @09:19:05.31 ATV.dial :AIV @09:19:05.65 AIV.dial :coilToMFB @09:19:05.66 AWV.select :waste @09:19:05.75 AS.reconfigure ASslow0 @09:19:05.79 AS.seek 0.000ml @09:19:11.76
PSV.dial :waste1 @09:19:13.07 PS.reconfigure PSconfig @09:19:13.10 PS.seek 0.000ml @09:19:26.20 PSV.dial :air @09:19:27.22 PS.seek 2.500ml @09:19:53.48 PSV.dial :mixloop @09:19:53.93 PS.reconfigure PSslow @09:19:53.96 PS.seek 0.000ml @09:20:45.29 PSV.dial :air @09:20:45.74 PS.reconfigure PSconfig @09:20:45.77 PS.seek 2.500ml @09:20:48.13 ATV.dial :air2 @09:20:48.70 AS.reconfigure ASslow1 @09:20:48.73 AS.seek 0.050ml @09:20:59.23 ATV.dial :AIV @09:20:59.81 AS.seek 0.010ml @09:21:08.23 ATV.dial :waste @09:21:09.61 AS.seek empty @09:21:11.94
PSV.dial :mixloop @09:21:12.02 ethanol wash @09:21:12.03 ATV.dial :air2 @09:21:12.39
PS.reconfigure PSslow @09:21:12.42 PS.seek 0.000ml @09:21:13.05 AS.reconfigure ASslow2 @09:21:13.08 AS.seek 0.009ml @09:21:23.76 delay 3 seconds @09:21:26.77 Pulling 0.4ml of Ethanol Wash @09:21:26.78 ATV.dial :ethanol @09:21:27.35 AS.reconfigure ASslow1 @09:21:27.38 AS.seek 0.409ml @09:22:03.69
Cleaning Collection Syringe @09:22:03.70 CSV.dial CSR @09:22:04.13 CSR.select :flush @09:22:04.23 CS.seek 4.000ml @09:22:15.25 delay 5 seconds @09:22:20.26 CSR.select :air @09:22:20.35 CS.seek 10.000ml @09:22:36.28 CSV.dial CTV @09:22:36.30
CTV.dial CSV @09:22:37.64 CBV.dial :waste1 @09:22:38.12 CS.seek 0.000ml @09:22:49.22 delay 3 seconds @09:22:52.23 ATV.dial :air1 @09:22:52.55 delay 3 seconds @09:22:55.61 AS.seek 0.434ml @09:23:00.91 delay 3 seconds @09:23:03.92 ATV.dial :ethanol @09:23:04.22
CSV.dial CSR @09:23:04.32 AS.seek 0.419ml @09:23:04.74
CSR.select :flush @09:23:04.84 CS.seek 4.000ml @09:23:07.63 delay 3 seconds @09:23:10.64 ATV.dial ASV @09:23:11.79 AS.seek 0.092ml @09:23:15.75
delay 5 seconds @09:23:20.76 CSR.select :air @09:23:20.85 CS.seek 10.000ml @09:23:36.84 CSV.dial CTV @09:23:36.86
CTV.dial CSV @09:23:37.39 CBV.dial :waste1 @09:23:37.41 CS.seek 0.000ml @09:24:03.58 Garaging FlushPuck in Collection Clamp @09:24:03.63 Elbow.reconfigure TightElbow @09:24:03.66 Elbow.seek CC @09:24:03.75 Elbow.reconfigure LooseElbow @09:24:03.83 Forearm.seek CC @09:24:04.79 CC.seek sealed @09:24:05.72 Hand.grip @09:24:06.43 CC.seek open @09:24:09.71 Forearm.seek retract @09:24:10.66 Elbow.reconfigure TightElbow @09:24:10.70 Elbow.seek garage @09:24:13.53 Elbow.reconfigure LooseElbow @09:24:13.61 Forearm.reconfigure TightForearm @09:24:13.64 Forearm.seek garage @09:24:14.81 Hand.release @09:24:15.60 Forearm.reconfigure LooseForearm @09:24:15.63 Forearm.seek retract @09:24:16.64 Whole Cell Archive sampling 1000ml @09:24:16.66 Loading wcr puck from tube 2 into Collection Clamp @09:24:16.83 SC.reconfigure SCtight @09:24:16.87 SC.seek 2 @09:24:18.70 delay 3 seconds @09:24:19.07 SC.reconfigure SCloose @09:24:19.25 Elbow.reconfigure TightElbow @09:24:19.28 Elbow.seek 2 @09:24:21.11 Forearm.reconfigure TightForearm @09:24:21.14 Forearm.seek 2 + 800 counts @09:24:21.71 ATV.dial :waste2 @09:24:22.22 SE.reconfigure SEpick @09:24:22.27 AS.reconfigure ASconfig @09:24:22.30 SE.seek beamBreak @09:24:22.33 AS.seek empty @09:24:24.14 SE.configure SEconfig @09:24:24.26 Elbow.seek 2 @09:24:24.41 Forearm.seek 2 + 300 counts @09:24:24.67 Forearm.reconfigure LooseForearm @09:24:24.71 Hand.grip @09:24:25.45 SE.seek down @09:24:25.57 Forearm.seek retract @09:24:26.57 Elbow.seek CC @09:24:26.93 SE.coast @09:24:28.05 ATV.dial :air2 @09:24:28.15 Elbow.reconfigure LooseElbow @09:24:28.23 Forearm.reconfigure TightForearm @09:24:28.85 Forearm.seek CC @09:24:28.90 AS.reconfigure ASslow1 @09:24:28.94 AS.seek 1.000ml @09:24:29.99 CC.seek closed @09:24:33.81 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.98 @09:24:33.82 Hand.release @09:24:34.59 Forearm.reconfigure LooseForearm @09:24:34.62 Forearm.seek retract @09:24:35.58 SC.coast @09:24:35.61 collectSample 1000ml w/bubble point of 55psi @09:24:35.63 Priming 7ml Sleepy.power :sampler,:ON @09:24:35.64 Intake.select :close @09:24:35.65 Exhaust.select :close @09:24:35.66 SS.hold @09:24:35.81 Calibrating pressure sensors @09:24:35.82 CTV.dial :air @09:24:37.13 SSV.dial :spit,avoiding => exhaust @09:24:37.14 SS.reconfigure SSfast @09:24:37.17 SS.seek 5.00ml @09:24:39.78 SSV.dial CBV @09:24:39.79 CBV.dial SSV @09:24:41.18 delay 3.8 seconds @09:24:45.07 SSV.dial :spit @09:24:46.41 SS.seek 0.00ml @09:24:49.01 SSV.dial CBV @09:24:50.32 CTV.dial :intake @09:24:51.04 Waiting for external sampler to finish priming... @09:24:51.05 delayUntil "09:34:35.63" @09:27:53.32 delay 5 seconds @09:27:58.33 ATV.dial ASV @09:27:59.04 ASV.dial :SPEdelivery @09:27:59.06 AS.seek 0.020ml @09:31:19.21 delay 5 seconds @09:31:24.22 ATV.dial :air2 @09:31:24.99 AS.seek 0.520ml @09:33:07.25 delay 5 seconds @09:33:12.26 ATV.dial ASV @09:33:12.98 ASV.dial :wasteR @09:33:13.74 AS.seek 0.300ml @09:33:58.87 delay 20 seconds @09:34:18.88 AS.seek 0.070ml @09:34:35.64 SSV.dial CBV @09:34:35.66 CBV.dial SSV @09:34:35.67 Allowing 55s for 7.0ml of remaining 7.0ml @09:34:35.68 Intake.select :open @09:34:37.81 Intake @34.8psia, vacuum from 20.0 to 24.0psi @09:34:37.83 SS.reconfigure forSampling @09:34:37.86 SS.seek 7.00ml @09:34:40.06 Vacuum too high -- waiting up to 40s @09:34:41.64 Intake @33.4psia, vacuum from 20.0 to 24.0psi @09:34:41.66 SS.reconfigure forSampling @09:34:41.69 SS.seek 7.00ml @09:34:44.15 Vacuum too high -- waiting up to 40s @09:34:45.74 Intake @32.8psia, vacuum from 20.0 to 24.0psi @09:34:45.75 SS.reconfigure forSampling @09:34:45.79 SS.seek 7.00ml @09:34:48.24 Stroke completed -- waiting for pressure to equalize ... @09:34:52.41 Intake.select :close @09:34:52.55 SSV.dial :exhaust @09:34:53.32 Exhaust.select :open @09:34:53.42 SS.reconfigure SSexhale @09:34:53.46 SS.seek 0.00ml @09:34:57.25 Exhaust.select :close @09:34:57.36 Sampling 1000.0ml @09:34:57.37 Intake.select :close @09:34:57.38 Exhaust.select :close @09:34:57.39 SS.reconfigure SSconfig @09:34:57.42 SS.hold @09:34:57.58 CTV.dial :intake @09:34:57.59 SSV.dial CBV @09:34:57.61 CBV.dial SSV @09:34:58.40 Allowing 185s for 25.0ml of remaining 1000.0ml @09:34:58.41 Intake.select :open @09:35:00.54 Intake @35.7psia, vacuum from 20.0 to 24.0psi @09:35:00.56 SS.reconfigure forSampling @09:35:00.59 SS.seek 25.00ml @09:35:03.46 Vacuum too high -- waiting up to 40s @09:35:03.95 Intake @32.5psia, vacuum from 20.0 to 24.0psi @09:35:03.97 SS.reconfigure forSampling @09:35:04.00 SS.seek 25.00ml @09:35:06.04 delay 20 seconds @09:35:06.15 Vacuum too high -- waiting up to 40s @09:35:06.64 Intake @31.9psia, vacuum from 20.0 to 24.0psi @09:35:06.66 SS.reconfigure forSampling @09:35:06.69 SS.seek 25.00ml @09:35:08.77 Vacuum too high -- waiting up to 40s @09:35:09.48 Intake @33.2psia, vacuum from 20.0 to 24.0psi @09:35:09.50 SS.reconfigure forSampling @09:35:09.53 SS.seek 25.00ml @09:35:12.27 Vacuum too high -- waiting up to 40s @09:35:12.76 Intake @32.7psia, vacuum from 20.0 to 24.0psi @09:35:12.78 SS.reconfigure forSampling @09:35:12.81 SS.seek 25.00ml @09:35:22.40 Vacuum too high -- waiting up to 40s @09:35:22.89 Intake @32.8psia, vacuum from 20.0 to 24.0psi @09:35:22.90 SS.reconfigure forSampling @09:35:22.94 SS.seek 25.00ml @09:35:26.05 ATV.dial :waste2 @09:35:26.69 AS.reconfigure ASconfig @09:35:26.72 AS.seek empty @09:35:31.15 Elution @09:35:31.16 ATV.dial :air1 @09:35:32.23 AS.reconfigure ASslow1 @09:35:32.26 AS.seek 0.049ml @09:35:37.80 Stroke completed -- waiting for pressure to equalize ... @09:35:39.03 Intake.select :close @09:35:39.16 SSV.dial :exhaust @09:35:39.94 Exhaust.select :open @09:35:40.04 SS.reconfigure SSexhale @09:35:40.08 SS.seek 0.00ml @09:35:42.74 delay 3 seconds @09:35:45.75 ATV.dial :ElutionWater @09:35:46.32 AS.seek 0.109ml @09:35:48.91 Exhaust.select :close @09:35:49.01 SSV.dial CBV @09:35:49.03 CBV.dial SSV @09:35:49.80 Allowing 185s for 25.0ml of remaining 975.0ml @09:35:49.81 Intake.select :open @09:35:51.95 Intake @35.2psia, vacuum from 20.0 to 24.0psi @09:35:51.97 SS.reconfigure forSampling @09:35:52.00 SS.seek 25.00ml @09:35:58.80 delay 3 seconds @09:36:01.81 ATV.dial ASV,via => air2 @09:36:03.46 ASV.dial :SPEelution @09:36:03.96 AFV.select :carrierSolution @09:36:04.11 AS.reconfigure ASnorm @09:36:04.14 AS.seek 0.759ml @09:36:28.29 Vacuum too high -- waiting up to 40s @09:36:28.56 Intake @33.6psia, vacuum from 20.0 to 24.0psi @09:36:28.58 SS.reconfigure forSampling @09:36:28.61 SS.seek 25.00ml @09:36:33.90 Stroke completed -- waiting for pressure to equalize ... @09:36:34.54 Intake.select :close @09:36:34.68 SSV.dial :exhaust @09:36:35.44 Exhaust.select :open @09:36:35.54 SS.reconfigure SSexhale @09:36:35.58 SS.seek 0.00ml @09:36:37.63 delay 3 seconds @09:36:40.64 AFV.select :holdingCoil @09:36:40.78 AS.reconfigure ASslow1 @09:36:40.81 AS.seek 0.489ml @09:36:44.20 Exhaust.select :close @09:36:44.30 SSV.dial CBV @09:36:44.32 CBV.dial SSV @09:36:45.09 Allowing 185s for 25.0ml of remaining 950.0ml @09:36:45.10 Intake.select :open @09:36:47.24 Intake @35.6psia, vacuum from 20.0 to 24.0psi @09:36:47.26 SS.reconfigure forSampling @09:36:47.29 SS.seek 25.00ml @09:37:36.11 delay 5 seconds @09:37:41.17 AS.reconfigure ASslow2 @09:37:41.20 AS.seek 0.454ml @09:37:46.46 Stroke completed -- waiting for pressure to equalize ... @09:37:47.69 Intake.select :close @09:37:47.82 SSV.dial :exhaust @09:37:48.57 Exhaust.select :open @09:37:48.67 SS.reconfigure SSexhale @09:37:48.71 SS.seek 0.00ml @09:37:57.53 Exhaust.select :close @09:37:57.63 SSV.dial CBV @09:37:57.65 CBV.dial SSV @09:37:58.42 Allowing 185s for 25.0ml of remaining 925.0ml @09:37:58.43 Intake.select :open @09:38:00.57 Intake @36.5psia, vacuum from 20.0 to 24.0psi @09:38:00.59 SS.reconfigure forSampling @09:38:00.62 SS.seek 25.00ml @09:38:17.33 delay 1:00 @09:38:59.76 Stroke completed -- waiting for pressure to equalize ... @09:39:01.56 Intake.select :close @09:39:01.69 SSV.dial :exhaust @09:39:02.46 Exhaust.select :open @09:39:02.56 SS.reconfigure SSexhale @09:39:02.60 SS.seek 0.00ml @09:39:11.37 Exhaust.select :close @09:39:11.47 SSV.dial CBV @09:39:11.49 CBV.dial SSV @09:39:12.27 Allowing 185s for 25.0ml of remaining 900.0ml @09:39:12.29 Intake.select :open @09:39:14.42 Intake @36.2psia, vacuum from 20.0 to 24.0psi @09:39:14.44 SS.reconfigure forSampling @09:39:14.47 SS.seek 25.00ml @09:39:17.34 pushing eluate to holding coil @09:39:17.40 AS.seek 0.294ml @09:40:13.69 Stroke completed -- waiting for pressure to equalize ... @09:40:14.93 Intake.select :close @09:40:15.06 SSV.dial :exhaust @09:40:15.83 Exhaust.select :open @09:40:15.93 SS.reconfigure SSexhale @09:40:15.97 SS.seek 0.00ml @09:40:25.05 Exhaust.select :close @09:40:25.15 SSV.dial CBV @09:40:25.17 CBV.dial SSV @09:40:25.97 Allowing 185s for 25.0ml of remaining 875.0ml @09:40:25.98 Intake.select :open @09:40:28.11 Intake @36.0psia, vacuum from 20.0 to 24.0psi @09:40:28.13 SS.reconfigure forSampling @09:40:28.16 SS.seek 25.00ml @09:41:03.63 Vacuum too high -- waiting up to 40s @09:41:03.89 Intake @34.5psia, vacuum from 20.0 to 24.0psi @09:41:03.90 SS.reconfigure forSampling @09:41:03.94 SS.seek 25.00ml @09:41:48.15 Stroke completed -- waiting for pressure to equalize ... @09:41:49.38 Intake.select :close @09:41:49.51 SSV.dial :exhaust @09:41:50.28 Exhaust.select :open @09:41:50.38 SS.reconfigure SSexhale @09:41:50.42 SS.seek 0.00ml @09:41:59.04 Exhaust.select :close @09:41:59.14 SSV.dial CBV @09:41:59.16 CBV.dial SSV @09:41:59.96 Allowing 185s for 25.0ml of remaining 850.0ml @09:41:59.97 Intake.select :open @09:42:00.76 delay 30 seconds @09:42:02.10 Intake @35.7psia, vacuum from 20.0 to 24.0psi @09:42:02.12 SS.reconfigure forSampling @09:42:02.15 SS.seek 25.00ml @09:42:30.77 ATV.dial :waste,via => air3 @09:42:32.22 AS.reconfigure ASconfig @09:42:32.25 AS.seek empty @09:42:49.74 Parking Bleach in LysateSource line and SPEcolumn @09:42:49.75 ATV.dial :air3 @09:42:50.31 AS.reconfigure ASslow1 @09:42:50.34 AS.seek 0.029ml @09:42:56.73 ATV.dial :ElutionWater @09:42:57.11 AS.seek 0.059ml @09:43:03.46 ATV.dial :air3 @09:43:03.84 AS.seek 0.069ml @09:43:06.13 delay 2 seconds @09:43:08.14 ATV.dial :bleach @09:43:08.51 AS.seek 0.711ml @09:43:52.43 Stroke completed -- waiting for pressure to equalize ... @09:43:53.66 Intake.select :close @09:43:53.80 SSV.dial :exhaust @09:43:54.57 Exhaust.select :open @09:43:54.67 SS.reconfigure SSexhale @09:43:54.71 SS.seek 0.00ml @09:44:03.57 Exhaust.select :close @09:44:03.67 SSV.dial CBV @09:44:03.69 CBV.dial SSV @09:44:04.46 Allowing 185s for 25.0ml of remaining 825.0ml @09:44:04.47 Intake.select :open @09:44:06.61 Intake @35.9psia, vacuum from 20.0 to 24.0psi @09:44:06.63 SS.reconfigure forSampling @09:44:06.66 SS.seek 25.00ml @09:45:19.68 delay 3 seconds @09:45:22.69 ATV.dial :air3 @09:45:23.11 delay 3 seconds @09:45:26.17 AS.seek 0.736ml @09:45:31.52 delay 3 seconds @09:45:34.53 ATV.dial :bleach @09:45:34.91 AS.seek 0.721ml @09:45:38.22 delay 3 seconds @09:45:41.23 ATV.dial :AIV @09:45:42.31 AS.reconfigure ASconfig @09:45:42.34 AS.seek 0.015ml @09:45:56.95 Stroke completed -- waiting for pressure to equalize ... @09:45:58.17 Intake.select :close @09:45:58.30 SSV.dial :exhaust @09:45:59.06 Exhaust.select :open @09:45:59.16 SS.reconfigure SSexhale @09:45:59.20 SS.seek 0.00ml @09:46:08.08 Exhaust.select :close @09:46:08.18 SSV.dial CBV @09:46:08.20 CBV.dial SSV @09:46:08.97 Allowing 185s for 25.0ml of remaining 800.0ml @09:46:08.98 Intake.select :open @09:46:11.12 Intake @36.8psia, vacuum from 20.0 to 24.0psi @09:46:11.14 SS.reconfigure forSampling @09:46:11.17 SS.seek 25.00ml @09:46:23.89 ATV.dial :waste @09:46:25.70 AS.seek empty @09:46:26.91 ATV.dial :air3 @09:46:27.48 AS.reconfigure ASslow1 @09:46:27.52 AS.seek 0.009ml @09:46:29.87 delay 2 seconds @09:46:31.88 ATV.dial :bleach @09:46:32.26 AS.seek 0.579ml @09:48:01.46 Stroke completed -- waiting for pressure to equalize ... @09:48:02.10 Intake.select :close @09:48:02.24 SSV.dial :exhaust @09:48:03.01 Exhaust.select :open @09:48:03.11 SS.reconfigure SSexhale @09:48:03.15 SS.seek 0.00ml @09:48:11.89 Exhaust.select :close @09:48:11.99 SSV.dial CBV @09:48:12.01 CBV.dial SSV @09:48:12.79 Allowing 185s for 25.0ml of remaining 775.0ml @09:48:12.81 Intake.select :open @09:48:14.94 Intake @36.6psia, vacuum from 20.0 to 24.0psi @09:48:14.96 SS.reconfigure forSampling @09:48:14.99 SS.seek 25.00ml @09:48:28.73 delay 3 seconds @09:48:31.74 ATV.dial :air3 @09:48:32.08 delay 3 seconds @09:48:35.14 AS.seek 0.604ml @09:48:40.63 delay 3 seconds @09:48:43.65 ATV.dial :bleach @09:48:44.05 AS.seek 0.589ml @09:48:47.36 delay 3 seconds @09:48:50.37 ATV.dial :ASV @09:48:51.26 ASV.dial :wasteR @09:48:51.93 AS.seek 0.000ml @09:50:05.92 Stroke completed -- waiting for pressure to equalize ... @09:50:06.56 Intake.select :close @09:50:06.70 SSV.dial :exhaust @09:50:07.47 Exhaust.select :open @09:50:07.57 SS.reconfigure SSexhale @09:50:07.61 SS.seek 0.00ml @09:50:16.53 Exhaust.select :close @09:50:16.63 SSV.dial CBV @09:50:16.65 CBV.dial SSV @09:50:17.44 Allowing 185s for 25.0ml of remaining 750.0ml @09:50:17.45 Intake.select :open @09:50:19.59 Intake @37.3psia, vacuum from 20.0 to 24.0psi @09:50:19.61 SS.reconfigure forSampling @09:50:19.64 SS.seek 25.00ml @09:50:52.32 delay 1:00 @09:51:52.33 AFV.select :carrierSolution @09:51:52.42 delay 2 seconds @09:51:54.43 AS.reconfigure ASconfig @09:51:54.46 AS.seek 1.000ml @09:52:09.90 Stroke completed -- waiting for pressure to equalize ... @09:52:11.70 Intake.select :close @09:52:11.83 SSV.dial :exhaust @09:52:12.60 Exhaust.select :open @09:52:12.70 SS.reconfigure SSexhale @09:52:12.74 SS.seek 0.00ml @09:52:21.54 Exhaust.select :close @09:52:21.64 SSV.dial CBV @09:52:21.66 CBV.dial SSV @09:52:22.45 Allowing 185s for 25.0ml of remaining 725.0ml @09:52:22.46 Intake.select :open @09:52:24.60 Intake @36.7psia, vacuum from 20.0 to 24.0psi @09:52:24.62 SS.reconfigure forSampling @09:52:24.65 SS.seek 25.00ml @09:52:53.08 delay 1 second @09:52:54.09 AFV.select :holdingCoil @09:52:54.18 delay 1 second @09:52:55.19 ATV.dial :ASV @09:52:55.20 ASV.dial :wasteR @09:52:55.21 AS.reconfigure ASslow1 @09:52:55.24 AS.seek 0.000ml @09:54:06.04 Vacuum too high -- waiting up to 40s @09:54:06.30 Intake @36.8psia, vacuum from 20.0 to 24.0psi @09:54:06.31 SS.reconfigure forSampling @09:54:06.35 SS.seek 25.00ml @09:54:10.41 Vacuum too high -- waiting up to 40s @09:54:10.69 Intake @37.3psia, vacuum from 20.0 to 24.0psi @09:54:10.71 SS.reconfigure forSampling @09:54:10.74 SS.seek 25.00ml @09:54:13.76 Vacuum too high -- waiting up to 40s @09:54:14.02 Intake @37.2psia, vacuum from 20.0 to 24.0psi @09:54:14.04 SS.reconfigure forSampling @09:54:14.07 SS.seek 25.00ml @09:54:16.58 Stroke completed -- waiting for pressure to equalize ... @09:54:18.38 Intake.select :close @09:54:18.51 SSV.dial :exhaust @09:54:19.28 Exhaust.select :open @09:54:19.38 SS.reconfigure SSexhale @09:54:19.42 SS.seek 0.00ml @09:54:28.20 Exhaust.select :close @09:54:28.30 SSV.dial CBV @09:54:28.32 CBV.dial SSV @09:54:29.11 Allowing 185s for 25.0ml of remaining 700.0ml @09:54:29.12 Intake.select :open @09:54:31.26 Intake @36.7psia, vacuum from 20.0 to 24.0psi @09:54:31.28 SS.reconfigure forSampling @09:54:31.31 SS.seek 25.00ml @09:54:35.41 Vacuum too high -- waiting up to 40s @09:54:35.67 Intake @36.7psia, vacuum from 20.0 to 24.0psi @09:54:35.68 SS.reconfigure forSampling @09:54:35.72 SS.seek 25.00ml @09:54:40.16 Vacuum too high -- waiting up to 40s @09:54:40.43 Intake @36.6psia, vacuum from 20.0 to 24.0psi @09:54:40.45 SS.reconfigure forSampling @09:54:40.48 SS.seek 25.00ml @09:54:43.76 Vacuum too high -- waiting up to 40s @09:54:44.03 Intake @36.5psia, vacuum from 20.0 to 24.0psi @09:54:44.04 SS.reconfigure forSampling @09:54:44.07 SS.seek 25.00ml @09:54:46.79 Vacuum too high -- waiting up to 40s @09:54:47.07 Intake @36.4psia, vacuum from 20.0 to 24.0psi @09:54:47.08 SS.reconfigure forSampling @09:54:47.11 SS.seek 25.00ml @09:54:50.97 Vacuum too high -- waiting up to 40s @09:54:51.25 Intake @36.5psia, vacuum from 20.0 to 24.0psi @09:54:51.27 SS.reconfigure forSampling @09:54:51.30 SS.seek 25.00ml @09:54:54.78 Vacuum too high -- waiting up to 40s @09:54:55.05 Intake @37.0psia, vacuum from 20.0 to 24.0psi @09:54:55.07 SS.reconfigure forSampling @09:54:55.10 SS.seek 25.00ml @09:54:57.51 Vacuum too high -- waiting up to 40s @09:54:57.78 Intake @36.8psia, vacuum from 20.0 to 24.0psi @09:54:57.80 SS.reconfigure forSampling @09:54:57.83 SS.seek 25.00ml @09:55:00.32 Vacuum too high -- waiting up to 40s @09:55:00.59 Intake @36.3psia, vacuum from 20.0 to 24.0psi @09:55:00.61 SS.reconfigure forSampling @09:55:00.64 SS.seek 25.00ml @09:55:03.22 Vacuum too high -- waiting up to 40s @09:55:03.50 Intake @36.4psia, vacuum from 20.0 to 24.0psi @09:55:03.51 SS.reconfigure forSampling @09:55:03.54 SS.seek 25.00ml @09:55:06.07 Vacuum too high -- waiting up to 40s @09:55:06.34 Intake @37.0psia, vacuum from 20.0 to 24.0psi @09:55:06.36 SS.reconfigure forSampling @09:55:06.39 SS.seek 25.00ml @09:55:08.50 Vacuum too high -- waiting up to 40s @09:55:08.77 Intake @36.2psia, vacuum from 20.0 to 24.0psi @09:55:08.79 SS.reconfigure forSampling @09:55:08.82 SS.seek 25.00ml @09:55:11.19 Vacuum too high -- waiting up to 40s @09:55:11.46 Intake @36.7psia, vacuum from 20.0 to 24.0psi @09:55:11.48 SS.reconfigure forSampling @09:55:11.51 SS.seek 25.00ml @09:55:13.72 Vacuum too high -- waiting up to 40s @09:55:13.99 Intake @36.2psia, vacuum from 20.0 to 24.0psi @09:55:14.01 SS.reconfigure forSampling @09:55:14.04 SS.seek 25.00ml @09:55:16.60 Vacuum too high -- waiting up to 40s @09:55:16.87 Intake @36.9psia, vacuum from 20.0 to 24.0psi @09:55:16.89 SS.reconfigure forSampling @09:55:16.92 SS.seek 25.00ml @09:55:18.66 Vacuum too high -- waiting up to 40s @09:55:18.93 Intake @36.3psia, vacuum from 20.0 to 24.0psi @09:55:18.94 SS.reconfigure forSampling @09:55:18.98 SS.seek 25.00ml @09:55:21.22 Vacuum too high -- waiting up to 40s @09:55:21.49 Intake @36.5psia, vacuum from 20.0 to 24.0psi @09:55:21.50 SS.reconfigure forSampling @09:55:21.54 SS.seek 25.00ml @09:55:23.47 Vacuum too high -- waiting up to 40s @09:55:23.74 Intake @36.5psia, vacuum from 20.0 to 24.0psi @09:55:23.76 SS.reconfigure forSampling @09:55:23.79 SS.seek 25.00ml @09:55:25.72 Vacuum too high -- waiting up to 40s @09:55:25.99 Intake @36.3psia, vacuum from 20.0 to 24.0psi @09:55:26.00 SS.reconfigure forSampling @09:55:26.04 SS.seek 25.00ml @09:55:28.03 Vacuum too high -- waiting up to 40s @09:55:28.30 Intake @36.7psia, vacuum from 20.0 to 24.0psi @09:55:28.31 SS.reconfigure forSampling @09:55:28.35 SS.seek 25.00ml @09:55:30.16 Vacuum too high -- waiting up to 40s @09:55:30.43 Intake @36.6psia, vacuum from 20.0 to 24.0psi @09:55:30.44 SS.reconfigure forSampling @09:55:30.48 SS.seek 25.00ml @09:55:32.22 Vacuum too high -- waiting up to 40s @09:55:32.49 Intake @36.2psia, vacuum from 20.0 to 24.0psi @09:55:32.50 SS.reconfigure forSampling @09:55:32.54 SS.seek 25.00ml @09:55:34.25 Vacuum too high -- waiting up to 40s @09:55:34.52 Intake @36.1psia, vacuum from 20.0 to 24.0psi @09:55:34.54 SS.reconfigure forSampling @09:55:34.57 SS.seek 25.00ml @09:55:36.47 Vacuum too high -- waiting up to 40s @09:55:36.74 Intake @36.0psia, vacuum from 20.0 to 24.0psi @09:55:36.75 SS.reconfigure forSampling @09:55:36.79 SS.seek 25.00ml @09:55:39.18 Vacuum too high -- waiting up to 40s @09:55:39.46 Intake @36.8psia, vacuum from 20.0 to 24.0psi @09:55:39.47 SS.reconfigure forSampling @09:55:39.50 SS.seek 25.00ml @09:55:42.00 Vacuum too high -- waiting up to 40s @09:55:42.27 Intake @36.5psia, vacuum from 20.0 to 24.0psi @09:55:42.29 SS.reconfigure forSampling @09:55:42.32 SS.seek 25.00ml @09:55:43.97 Vacuum too high -- waiting up to 40s @09:55:44.24 Intake @36.0psia, vacuum from 20.0 to 24.0psi @09:55:44.26 SS.reconfigure forSampling @09:55:44.29 SS.seek 25.00ml @09:55:45.91 Vacuum too high -- waiting up to 40s @09:55:46.19 Intake @36.3psia, vacuum from 20.0 to 24.0psi @09:55:46.20 SS.reconfigure forSampling @09:55:46.23 SS.seek 25.00ml @09:55:47.82 Vacuum too high -- waiting up to 40s @09:55:48.10 SS.seek 25.00ml @09:55:49.85 Vacuum too high -- waiting up to 40s @09:55:50.12 Intake @36.9psia, vacuum from 20.0 to 24.0psi @09:55:50.14 SS.reconfigure forSampling @09:55:50.17 SS.seek 25.00ml @09:55:51.91 Vacuum too high -- waiting up to 40s @09:55:52.18 Intake @37.0psia, vacuum from 20.0 to 24.0psi @09:55:52.20 SS.reconfigure forSampling @09:55:52.23 SS.seek 25.00ml @09:55:53.72 Vacuum too high -- waiting up to 40s @09:55:53.99 Intake @36.9psia, vacuum from 20.0 to 24.0psi @09:55:54.01 SS.reconfigure forSampling @09:55:54.04 SS.seek 25.00ml @09:55:55.64 Vacuum too high -- waiting up to 40s @09:55:55.91 Intake @36.8psia, vacuum from 20.0 to 24.0psi @09:55:55.93 SS.reconfigure forSampling @09:55:55.96 SS.seek 25.00ml @09:55:57.44 Vacuum too high -- waiting up to 40s @09:55:57.71 Intake @36.2psia, vacuum from 20.0 to 24.0psi @09:55:57.72 SS.reconfigure forSampling @09:55:57.76 SS.seek 25.00ml @09:55:59.31 Vacuum too high -- waiting up to 40s @09:55:59.58 Intake @36.3psia, vacuum from 20.0 to 24.0psi @09:55:59.59 SS.reconfigure forSampling @09:55:59.63 SS.seek 25.00ml @09:56:01.38 Vacuum too high -- waiting up to 40s @09:56:01.65 Intake @36.5psia, vacuum from 20.0 to 24.0psi @09:56:01.66 SS.reconfigure forSampling @09:56:01.70 SS.seek 25.00ml @09:56:03.35 Vacuum too high -- waiting up to 40s @09:56:03.62 Intake @36.9psia, vacuum from 20.0 to 24.0psi @09:56:03.64 SS.reconfigure forSampling @09:56:03.67 SS.seek 25.00ml @09:56:05.25 Vacuum too high -- waiting up to 40s @09:56:05.52 Intake @37.1psia, vacuum from 20.0 to 24.0psi @09:56:05.53 SS.reconfigure forSampling @09:56:05.57 SS.seek 25.00ml @09:56:07.03 Vacuum too high -- waiting up to 40s @09:56:07.30 Intake @37.0psia, vacuum from 20.0 to 24.0psi @09:56:07.31 SS.reconfigure forSampling @09:56:07.35 SS.seek 25.00ml @09:56:08.72 Vacuum too high -- waiting up to 40s @09:56:08.99 Intake @37.1psia, vacuum from 20.0 to 24.0psi @09:56:09.00 SS.reconfigure forSampling @09:56:09.04 SS.seek 25.00ml @09:56:10.38 Vacuum too high -- waiting up to 40s @09:56:10.65 Intake @36.9psia, vacuum from 20.0 to 24.0psi @09:56:10.66 SS.reconfigure forSampling @09:56:10.70 SS.seek 25.00ml @09:56:12.28 Vacuum too high -- waiting up to 40s @09:56:12.55 Intake @36.3psia, vacuum from 20.0 to 24.0psi @09:56:12.56 SS.reconfigure forSampling @09:56:12.60 SS.seek 25.00ml @09:56:14.25 Vacuum too high -- waiting up to 40s @09:56:14.52 Intake @36.8psia, vacuum from 20.0 to 24.0psi @09:56:14.53 SS.reconfigure forSampling @09:56:14.57 SS.seek 25.00ml @09:56:16.19 Vacuum too high -- waiting up to 40s @09:56:16.46 Intake @37.3psia, vacuum from 20.0 to 24.0psi @09:56:16.47 SS.reconfigure forSampling @09:56:16.51 SS.seek 25.00ml @09:56:17.81 Vacuum too high -- waiting up to 40s @09:56:18.08 Intake @36.7psia, vacuum from 20.0 to 24.0psi @09:56:18.09 SS.reconfigure forSampling @09:56:18.13 SS.seek 25.00ml @09:56:19.44 Vacuum too high -- waiting up to 40s @09:56:19.51 delay 20 seconds @09:56:19.70 Intake @36.4psia, vacuum from 20.0 to 24.0psi @09:56:19.71 SS.reconfigure forSampling @09:56:19.75 SS.seek 25.00ml @09:56:21.28 Vacuum too high -- waiting up to 40s @09:56:22.15 Intake @36.6psia, vacuum from 20.0 to 24.0psi @09:56:22.17 SS.reconfigure forSampling @09:56:22.20 SS.seek 25.00ml @09:56:24.28 Vacuum too high -- waiting up to 40s @09:56:24.56 Intake @36.9psia, vacuum from 20.0 to 24.0psi @09:56:24.57 SS.reconfigure forSampling @09:56:24.60 SS.seek 25.00ml @09:56:26.03 Vacuum too high -- waiting up to 40s @09:56:26.30 Intake @36.6psia, vacuum from 20.0 to 24.0psi @09:56:26.31 SS.reconfigure forSampling @09:56:26.35 SS.seek 25.00ml @09:56:27.84 Vacuum too high -- waiting up to 40s @09:56:28.12 SS.seek 25.00ml @09:56:29.38 Vacuum too high -- waiting up to 40s @09:56:29.64 Intake @36.3psia, vacuum from 20.0 to 24.0psi @09:56:29.66 SS.reconfigure forSampling @09:56:29.69 SS.seek 25.00ml @09:56:31.15 Vacuum too high -- waiting up to 40s @09:56:31.64 Intake @36.7psia, vacuum from 20.0 to 24.0psi @09:56:31.66 SS.reconfigure forSampling @09:56:31.69 SS.seek 25.00ml @09:56:33.53 Vacuum too high -- waiting up to 40s @09:56:34.02 Intake @37.4psia, vacuum from 20.0 to 24.0psi @09:56:34.04 SS.reconfigure forSampling @09:56:34.07 SS.seek 25.00ml @09:56:35.79 Vacuum too high -- waiting up to 40s @09:56:36.06 Intake @36.9psia, vacuum from 20.0 to 24.0psi @09:56:36.08 SS.reconfigure forSampling @09:56:36.11 SS.seek 25.00ml @09:56:37.69 Vacuum too high -- waiting up to 40s @09:56:37.96 Intake @37.0psia, vacuum from 20.0 to 24.0psi @09:56:37.98 SS.reconfigure forSampling @09:56:38.01 SS.seek 25.00ml @09:56:39.31 Vacuum too high -- waiting up to 40s @09:56:39.56 ATV.dial :air3 @09:56:39.61 Intake @37.1psia, vacuum from 20.0 to 24.0psi @09:56:39.63 SS.reconfigure forSampling @09:56:39.66 SS.seek 25.00ml @09:56:40.72 AS.seek 0.590ml @09:56:40.91 Vacuum too high -- waiting up to 40s @09:56:41.18 Intake @37.1psia, vacuum from 20.0 to 24.0psi @09:56:41.20 SS.reconfigure forSampling @09:56:41.23 SS.seek 25.00ml @09:56:42.62 Vacuum too high -- waiting up to 40s @09:56:42.89 Intake @37.3psia, vacuum from 20.0 to 24.0psi @09:56:42.91 SS.reconfigure forSampling @09:56:42.94 SS.seek 25.00ml @09:56:44.22 Vacuum too high -- waiting up to 40s @09:56:44.49 Intake @37.0psia, vacuum from 20.0 to 24.0psi @09:56:44.50 SS.reconfigure forSampling @09:56:44.54 SS.seek 25.00ml @09:56:45.84 Vacuum too high -- waiting up to 40s @09:56:46.12 SS.seek 25.00ml @09:56:47.44 Vacuum too high -- waiting up to 40s @09:56:47.71 Intake @37.2psia, vacuum from 20.0 to 24.0psi @09:56:47.73 SS.reconfigure forSampling @09:56:47.76 SS.seek 25.00ml @09:56:49.09 Vacuum too high -- waiting up to 40s @09:56:49.36 Intake @37.6psia, vacuum from 20.0 to 24.0psi @09:56:49.38 SS.reconfigure forSampling @09:56:49.41 SS.seek 25.00ml @09:56:50.66 Vacuum too high -- waiting up to 40s @09:56:50.93 Intake @37.3psia, vacuum from 20.0 to 24.0psi @09:56:50.95 SS.reconfigure forSampling @09:56:50.98 SS.seek 25.00ml @09:56:52.28 Vacuum too high -- waiting up to 40s @09:56:52.55 Intake @37.0psia, vacuum from 20.0 to 24.0psi @09:56:52.57 SS.reconfigure forSampling @09:56:52.60 SS.seek 25.00ml @09:56:53.94 Vacuum too high -- waiting up to 40s @09:56:54.21 Intake @37.0psia, vacuum from 20.0 to 24.0psi @09:56:54.22 SS.reconfigure forSampling @09:56:54.26 SS.seek 25.00ml @09:56:55.56 Vacuum too high -- waiting up to 40s @09:56:56.05 Intake @37.0psia, vacuum from 20.0 to 24.0psi @09:56:56.06 SS.reconfigure forSampling @09:56:56.10 SS.seek 25.00ml @09:56:57.70 Stroke completed -- waiting for pressure to equalize ... @09:57:00.09 Intake.select :close @09:57:00.22 SSV.dial :exhaust @09:57:01.57 Exhaust.select :open @09:57:01.67 SS.reconfigure SSexhale @09:57:01.71 SS.seek 0.00ml @09:57:10.34 Exhaust.select :close @09:57:10.44 SSV.dial CBV @09:57:10.46 CBV.dial SSV @09:57:11.24 Allowing 185s for 25.0ml of remaining 675.0ml @09:57:11.26 Intake.select :open @09:57:13.39 Intake @36.7psia, vacuum from 20.0 to 24.0psi @09:57:13.41 SS.reconfigure forSampling @09:57:13.44 SS.seek 25.00ml @09:57:15.80 Vacuum too high -- waiting up to 40s @09:57:16.07 Intake @36.5psia, vacuum from 20.0 to 24.0psi @09:57:16.08 SS.reconfigure forSampling @09:57:16.11 SS.seek 25.00ml @09:57:17.62 Vacuum too high -- waiting up to 40s @09:57:17.90 Intake @36.7psia, vacuum from 20.0 to 24.0psi @09:57:17.91 SS.reconfigure forSampling @09:57:17.94 SS.seek 25.00ml @09:57:19.31 Vacuum too high -- waiting up to 40s @09:57:19.59 Intake @36.8psia, vacuum from 20.0 to 24.0psi @09:57:19.60 SS.reconfigure forSampling @09:57:19.63 SS.seek 25.00ml @09:57:21.03 Vacuum too high -- waiting up to 40s @09:57:21.31 Intake @36.8psia, vacuum from 20.0 to 24.0psi @09:57:21.32 SS.reconfigure forSampling @09:57:21.35 SS.seek 25.00ml @09:57:22.65 Vacuum too high -- waiting up to 40s @09:57:22.93 Intake @36.4psia, vacuum from 20.0 to 24.0psi @09:57:22.95 SS.reconfigure forSampling @09:57:22.98 SS.seek 25.00ml @09:57:24.40 Vacuum too high -- waiting up to 40s @09:57:24.67 Intake @36.5psia, vacuum from 20.0 to 24.0psi @09:57:24.69 SS.reconfigure forSampling @09:57:24.72 SS.seek 25.00ml @09:57:26.09 Vacuum too high -- waiting up to 40s @09:57:26.58 Intake @37.0psia, vacuum from 20.0 to 24.0psi @09:57:26.60 SS.reconfigure forSampling @09:57:26.63 SS.seek 25.00ml @09:57:28.40 Vacuum too high -- waiting up to 40s @09:57:28.89 Intake @37.4psia, vacuum from 20.0 to 24.0psi @09:57:28.90 SS.reconfigure forSampling @09:57:28.94 SS.seek 25.00ml @09:57:30.56 Vacuum too high -- waiting up to 40s @09:57:30.83 Intake @36.8psia, vacuum from 20.0 to 24.0psi @09:57:30.84 SS.reconfigure forSampling @09:57:30.88 SS.seek 25.00ml @09:57:32.22 Vacuum too high -- waiting up to 40s @09:57:32.49 Intake @36.6psia, vacuum from 20.0 to 24.0psi @09:57:32.50 SS.reconfigure forSampling @09:57:32.54 SS.seek 25.00ml @09:57:33.93 Vacuum too high -- waiting up to 40s @09:57:34.20 Intake @36.7psia, vacuum from 20.0 to 24.0psi @09:57:34.21 SS.reconfigure forSampling @09:57:34.25 SS.seek 25.00ml @09:57:35.56 Vacuum too high -- waiting up to 40s @09:57:35.83 Intake @36.8psia, vacuum from 20.0 to 24.0psi @09:57:35.84 SS.reconfigure forSampling @09:57:35.88 SS.seek 25.00ml @09:57:37.28 Vacuum too high -- waiting up to 40s @09:57:37.76 Intake @37.6psia, vacuum from 20.0 to 24.0psi @09:57:37.77 SS.reconfigure forSampling @09:57:37.81 SS.seek 25.00ml @09:57:39.50 Vacuum too high -- waiting up to 40s @09:57:39.77 Intake @37.4psia, vacuum from 20.0 to 24.0psi @09:57:39.79 SS.reconfigure forSampling @09:57:39.82 SS.seek 25.00ml @09:57:41.12 Vacuum too high -- waiting up to 40s @09:57:41.39 Intake @37.2psia, vacuum from 20.0 to 24.0psi @09:57:41.40 SS.reconfigure forSampling @09:57:41.44 SS.seek 25.00ml @09:57:42.75 Vacuum too high -- waiting up to 40s @09:57:43.02 Intake @36.8psia, vacuum from 20.0 to 24.0psi @09:57:43.03 SS.reconfigure forSampling @09:57:43.07 SS.seek 25.00ml @09:57:44.18 Vacuum too high -- waiting up to 40s @09:57:44.45 Intake @36.8psia, vacuum from 20.0 to 24.0psi @09:57:44.47 SS.reconfigure forSampling @09:57:44.50 SS.seek 25.00ml @09:57:45.75 Vacuum too high -- waiting up to 40s @09:57:46.02 Intake @37.1psia, vacuum from 20.0 to 24.0psi @09:57:46.04 SS.reconfigure forSampling @09:57:46.07 SS.seek 25.00ml @09:57:47.34 Vacuum too high -- waiting up to 40s @09:57:47.83 Intake @37.3psia, vacuum from 20.0 to 24.0psi @09:57:47.84 SS.reconfigure forSampling @09:57:47.88 SS.seek 25.00ml @09:57:50.47 Vacuum too high -- waiting up to 40s @09:57:50.75 Intake @37.3psia, vacuum from 20.0 to 24.0psi @09:57:50.76 SS.reconfigure forSampling @09:57:50.79 SS.seek 25.00ml @09:57:52.09 Vacuum too high -- waiting up to 40s @09:57:52.36 Intake @37.1psia, vacuum from 20.0 to 24.0psi @09:57:52.38 SS.reconfigure forSampling @09:57:52.41 SS.seek 25.00ml @09:57:53.59 Vacuum too high -- waiting up to 40s @09:57:53.87 Intake @37.0psia, vacuum from 20.0 to 24.0psi @09:57:53.88 SS.reconfigure forSampling @09:57:53.91 SS.seek 25.00ml @09:57:55.25 Vacuum too high -- waiting up to 40s @09:57:55.53 Intake @37.2psia, vacuum from 20.0 to 24.0psi @09:57:55.54 SS.reconfigure forSampling @09:57:55.58 SS.seek 25.00ml @09:57:56.78 Vacuum too high -- waiting up to 40s @09:57:57.06 SS.seek 25.00ml @09:57:58.15 Vacuum too high -- waiting up to 40s @09:57:58.42 Intake @37.0psia, vacuum from 20.0 to 24.0psi @09:57:58.44 SS.reconfigure forSampling @09:57:58.47 SS.seek 25.00ml @09:57:59.68 Vacuum too high -- waiting up to 40s @09:58:00.11 Intake @37.4psia, vacuum from 20.0 to 24.0psi @09:58:00.12 SS.reconfigure forSampling @09:58:00.16 SS.seek 25.00ml @09:58:01.53 Vacuum too high -- waiting up to 40s @09:58:01.80 Intake @37.3psia, vacuum from 20.0 to 24.0psi @09:58:01.82 SS.reconfigure forSampling @09:58:01.85 SS.seek 25.00ml @09:58:02.96 Vacuum too high -- waiting up to 40s @09:58:03.24 Intake @37.4psia, vacuum from 20.0 to 24.0psi @09:58:03.25 SS.reconfigure forSampling @09:58:03.28 SS.seek 25.00ml @09:58:04.43 Vacuum too high -- waiting up to 40s @09:58:04.70 Intake @37.0psia, vacuum from 20.0 to 24.0psi @09:58:04.72 SS.reconfigure forSampling @09:58:04.75 SS.seek 25.00ml @09:58:05.96 Vacuum too high -- waiting up to 40s @09:58:06.23 Intake @37.2psia, vacuum from 20.0 to 24.0psi @09:58:06.25 SS.reconfigure forSampling @09:58:06.28 SS.seek 25.00ml @09:58:07.37 Vacuum too high -- waiting up to 40s @09:58:07.64 Intake @37.0psia, vacuum from 20.0 to 24.0psi @09:58:07.66 SS.reconfigure forSampling @09:58:07.69 SS.seek 25.00ml @09:58:08.78 Vacuum too high -- waiting up to 40s @09:58:09.05 Intake @36.8psia, vacuum from 20.0 to 24.0psi @09:58:09.07 SS.reconfigure forSampling @09:58:09.10 SS.seek 25.00ml @09:58:10.21 Vacuum too high -- waiting up to 40s @09:58:10.48 Intake @37.2psia, vacuum from 20.0 to 24.0psi @09:58:10.50 SS.reconfigure forSampling @09:58:10.53 SS.seek 25.00ml @09:58:11.65 Vacuum too high -- waiting up to 40s @09:58:11.92 Intake @37.5psia, vacuum from 20.0 to 24.0psi @09:58:11.93 SS.reconfigure forSampling @09:58:11.97 SS.seek 25.00ml @09:58:13.03 Vacuum too high -- waiting up to 40s @09:58:13.30 Intake @37.3psia, vacuum from 20.0 to 24.0psi @09:58:13.32 SS.reconfigure forSampling @09:58:13.35 SS.seek 25.00ml @09:58:14.50 Vacuum too high -- waiting up to 40s @09:58:14.77 Intake @36.8psia, vacuum from 20.0 to 24.0psi @09:58:14.79 SS.reconfigure forSampling @09:58:14.82 SS.seek 25.00ml @09:58:16.00 Vacuum too high -- waiting up to 40s @09:58:16.27 Intake @36.9psia, vacuum from 20.0 to 24.0psi @09:58:16.29 SS.reconfigure forSampling @09:58:16.32 SS.seek 25.00ml @09:58:17.43 Vacuum too high -- waiting up to 40s @09:58:17.92 Intake @37.0psia, vacuum from 20.0 to 24.0psi @09:58:17.93 SS.reconfigure forSampling @09:58:17.97 SS.seek 25.00ml @09:58:19.28 Vacuum too high -- waiting up to 40s @09:58:19.55 Intake @36.8psia, vacuum from 20.0 to 24.0psi @09:58:19.56 SS.reconfigure forSampling @09:58:19.60 SS.seek 25.00ml @09:58:20.78 Vacuum too high -- waiting up to 40s @09:58:21.05 Intake @37.1psia, vacuum from 20.0 to 24.0psi @09:58:21.07 SS.reconfigure forSampling @09:58:21.10 SS.seek 25.00ml @09:58:22.09 Vacuum too high -- waiting up to 40s @09:58:22.36 Intake @37.7psia, vacuum from 20.0 to 24.0psi @09:58:22.38 SS.reconfigure forSampling @09:58:22.41 SS.seek 25.00ml @09:58:24.81 Vacuum too high -- waiting up to 40s @09:58:25.08 Intake @36.8psia, vacuum from 20.0 to 24.0psi @09:58:25.10 SS.reconfigure forSampling @09:58:25.13 SS.seek 25.00ml @09:58:26.18 Vacuum too high -- waiting up to 40s @09:58:26.67 Intake @36.7psia, vacuum from 20.0 to 24.0psi @09:58:26.69 SS.reconfigure forSampling @09:58:26.72 SS.seek 25.00ml @09:58:28.18 Vacuum too high -- waiting up to 40s @09:58:28.68 Intake @36.7psia, vacuum from 20.0 to 24.0psi @09:58:28.70 SS.reconfigure forSampling @09:58:28.73 SS.seek 25.00ml @09:58:30.21 Vacuum too high -- waiting up to 40s @09:58:30.71 Intake @37.0psia, vacuum from 20.0 to 24.0psi @09:58:30.72 SS.reconfigure forSampling @09:58:30.75 SS.seek 25.00ml @09:58:32.18 Vacuum too high -- waiting up to 40s @09:58:32.67 Intake @37.5psia, vacuum from 20.0 to 24.0psi @09:58:32.69 SS.reconfigure forSampling @09:58:32.72 SS.seek 25.00ml @09:58:34.03 Vacuum too high -- waiting up to 40s @09:58:34.31 Intake @37.5psia, vacuum from 20.0 to 24.0psi @09:58:34.32 SS.reconfigure forSampling @09:58:34.35 SS.seek 25.00ml @09:58:35.40 Vacuum too high -- waiting up to 40s @09:58:35.89 Intake @37.2psia, vacuum from 20.0 to 24.0psi @09:58:35.90 SS.reconfigure forSampling @09:58:35.94 SS.seek 25.00ml @09:58:37.31 Vacuum too high -- waiting up to 40s @09:58:37.58 Intake @37.0psia, vacuum from 20.0 to 24.0psi @09:58:37.60 SS.reconfigure forSampling @09:58:37.63 SS.seek 25.00ml @09:58:38.68 Vacuum too high -- waiting up to 40s @09:58:38.95 Intake @36.9psia, vacuum from 20.0 to 24.0psi @09:58:38.97 SS.reconfigure forSampling @09:58:39.00 SS.seek 25.00ml @09:58:39.99 Vacuum too high -- waiting up to 40s @09:58:40.26 Intake @36.7psia, vacuum from 20.0 to 24.0psi @09:58:40.28 SS.reconfigure forSampling @09:58:40.31 SS.seek 25.00ml @09:58:41.38 Vacuum too high -- waiting up to 40s @09:58:41.40 delay 3 seconds @09:58:41.86 Intake @37.0psia, vacuum from 20.0 to 24.0psi @09:58:41.88 SS.reconfigure forSampling @09:58:41.91 SS.seek 25.00ml @09:58:43.21 Vacuum too high -- waiting up to 40s @09:58:43.70 Intake @37.3psia, vacuum from 20.0 to 24.0psi @09:58:43.72 SS.reconfigure forSampling @09:58:43.75 SS.seek 25.00ml @09:58:44.41 ATV.dial :ASV @09:58:45.12 Vacuum too high -- waiting up to 40s @09:58:45.40 SS.seek 25.00ml @09:58:45.60 ASV.dial :wasteR @09:58:45.66 AS.seek 0.090ml @09:58:46.40 Vacuum too high -- waiting up to 40s @09:58:46.67 Intake @36.7psia, vacuum from 20.0 to 24.0psi @09:58:46.69 SS.reconfigure forSampling @09:58:46.72 SS.seek 25.00ml @09:58:47.71 Vacuum too high -- waiting up to 40s @09:58:48.20 Intake @36.8psia, vacuum from 20.0 to 24.0psi @09:58:48.22 SS.reconfigure forSampling @09:58:48.25 SS.seek 25.00ml @09:58:49.59 Vacuum too high -- waiting up to 40s @09:58:49.86 Intake @37.2psia, vacuum from 20.0 to 24.0psi @09:58:49.89 SS.reconfigure forSampling @09:58:49.92 SS.seek 25.00ml @09:58:50.99 Vacuum too high -- waiting up to 40s @09:58:51.48 Intake @37.5psia, vacuum from 20.0 to 24.0psi @09:58:51.49 SS.reconfigure forSampling @09:58:51.53 SS.seek 25.00ml @09:58:52.77 Vacuum too high -- waiting up to 40s @09:58:53.04 Intake @37.0psia, vacuum from 20.0 to 24.0psi @09:58:53.05 SS.reconfigure forSampling @09:58:53.09 SS.seek 25.00ml @09:58:54.09 Vacuum too high -- waiting up to 40s @09:58:54.36 Intake @37.2psia, vacuum from 20.0 to 24.0psi @09:58:54.38 SS.reconfigure forSampling @09:58:54.41 SS.seek 25.00ml @09:58:55.40 Vacuum too high -- waiting up to 40s @09:58:56.28 Intake @37.5psia, vacuum from 20.0 to 24.0psi @09:58:56.30 SS.reconfigure forSampling @09:58:56.33 SS.seek 25.00ml @09:58:58.02 Vacuum too high -- waiting up to 40s @09:58:58.51 Intake @37.3psia, vacuum from 20.0 to 24.0psi @09:58:58.53 SS.reconfigure forSampling @09:58:58.56 SS.seek 25.00ml @09:58:59.77 Vacuum too high -- waiting up to 40s @09:59:00.05 Intake @37.2psia, vacuum from 20.0 to 24.0psi @09:59:00.06 SS.reconfigure forSampling @09:59:00.09 SS.seek 25.00ml @09:59:01.15 Vacuum too high -- waiting up to 40s @09:59:01.64 Intake @37.2psia, vacuum from 20.0 to 24.0psi @09:59:01.66 SS.reconfigure forSampling @09:59:01.69 SS.seek 25.00ml @09:59:02.99 Vacuum too high -- waiting up to 40s @09:59:03.26 Intake @36.8psia, vacuum from 20.0 to 24.0psi @09:59:03.28 SS.reconfigure forSampling @09:59:03.35 SS.seek 25.00ml @09:59:04.46 Vacuum too high -- waiting up to 40s @09:59:04.96 Intake @37.1psia, vacuum from 20.0 to 24.0psi @09:59:04.98 SS.reconfigure forSampling @09:59:05.01 SS.seek 25.00ml @09:59:06.21 Vacuum too high -- waiting up to 40s @09:59:06.70 Intake @37.5psia, vacuum from 20.0 to 24.0psi @09:59:06.72 SS.reconfigure forSampling @09:59:06.75 SS.seek 25.00ml @09:59:07.96 Vacuum too high -- waiting up to 40s @09:59:08.45 Intake @37.6psia, vacuum from 20.0 to 24.0psi @09:59:08.47 SS.reconfigure forSampling @09:59:08.50 SS.seek 25.00ml @09:59:09.74 Vacuum too high -- waiting up to 40s @09:59:10.01 Intake @37.4psia, vacuum from 20.0 to 24.0psi @09:59:10.03 SS.reconfigure forSampling @09:59:10.06 SS.seek 25.00ml @09:59:11.09 Vacuum too high -- waiting up to 40s @09:59:11.36 Intake @37.4psia, vacuum from 20.0 to 24.0psi @09:59:11.38 SS.reconfigure forSampling @09:59:11.41 SS.seek 25.00ml @09:59:12.30 Vacuum too high -- waiting up to 40s @09:59:12.57 Intake @37.2psia, vacuum from 20.0 to 24.0psi @09:59:12.59 SS.reconfigure forSampling @09:59:12.62 SS.seek 25.00ml @09:59:13.62 Vacuum too high -- waiting up to 40s @09:59:13.90 Intake @36.9psia, vacuum from 20.0 to 24.0psi @09:59:13.91 SS.reconfigure forSampling @09:59:13.94 SS.seek 25.00ml @09:59:14.93 Vacuum too high -- waiting up to 40s @09:59:15.42 Intake @37.2psia, vacuum from 20.0 to 24.0psi @09:59:15.44 SS.reconfigure forSampling @09:59:15.47 SS.seek 25.00ml @09:59:16.65 Vacuum too high -- waiting up to 40s @09:59:16.92 Intake @37.1psia, vacuum from 20.0 to 24.0psi @09:59:16.93 SS.reconfigure forSampling @09:59:16.97 SS.seek 25.00ml @09:59:17.87 Vacuum too high -- waiting up to 40s @09:59:18.14 Intake @36.9psia, vacuum from 20.0 to 24.0psi @09:59:18.15 SS.reconfigure forSampling @09:59:18.19 SS.seek 25.00ml @09:59:19.15 Vacuum too high -- waiting up to 40s @09:59:19.64 Intake @37.4psia, vacuum from 20.0 to 24.0psi @09:59:19.66 SS.reconfigure forSampling @09:59:19.69 SS.seek 25.00ml @09:59:20.87 Vacuum too high -- waiting up to 40s @09:59:21.14 Intake @37.4psia, vacuum from 20.0 to 24.0psi @09:59:21.16 SS.reconfigure forSampling @09:59:21.19 SS.seek 25.00ml @09:59:22.12 Vacuum too high -- waiting up to 40s @09:59:22.39 Intake @36.9psia, vacuum from 20.0 to 24.0psi @09:59:22.41 SS.reconfigure forSampling @09:59:22.44 SS.seek 25.00ml @09:59:23.34 Vacuum too high -- waiting up to 40s @09:59:23.61 Intake @37.1psia, vacuum from 20.0 to 24.0psi @09:59:23.63 SS.reconfigure forSampling @09:59:23.66 SS.seek 25.00ml @09:59:24.59 Vacuum too high -- waiting up to 40s @09:59:24.86 Intake @36.8psia, vacuum from 20.0 to 24.0psi @09:59:24.88 SS.reconfigure forSampling @09:59:24.91 SS.seek 25.00ml @09:59:25.93 Vacuum too high -- waiting up to 40s @09:59:27.05 Intake @37.2psia, vacuum from 20.0 to 24.0psi @09:59:27.06 SS.reconfigure forSampling @09:59:27.09 SS.seek 25.00ml @09:59:28.77 Vacuum too high -- waiting up to 40s @09:59:29.04 Intake @36.4psia, vacuum from 20.0 to 24.0psi @09:59:29.06 SS.reconfigure forSampling @09:59:29.09 SS.seek 25.00ml @09:59:30.09 Vacuum too high -- waiting up to 40s @09:59:30.37 Intake @36.6psia, vacuum from 20.0 to 24.0psi @09:59:30.39 SS.reconfigure forSampling @09:59:30.42 SS.seek 25.00ml @09:59:31.43 Vacuum too high -- waiting up to 40s @09:59:31.92 Intake @37.1psia, vacuum from 20.0 to 24.0psi @09:59:31.94 SS.reconfigure forSampling @09:59:31.97 SS.seek 25.00ml @09:59:33.30 Vacuum too high -- waiting up to 40s @09:59:33.80 Intake @37.1psia, vacuum from 20.0 to 24.0psi @09:59:33.82 SS.reconfigure forSampling @09:59:33.85 SS.seek 25.00ml @09:59:35.08 Vacuum too high -- waiting up to 40s @09:59:35.35 Intake @36.6psia, vacuum from 20.0 to 24.0psi @09:59:35.36 SS.reconfigure forSampling @09:59:35.40 SS.seek 25.00ml @09:59:36.33 Vacuum too high -- waiting up to 40s @09:59:36.62 Intake @36.0psia, vacuum from 20.0 to 24.0psi @09:59:36.63 SS.reconfigure forSampling @09:59:36.66 SS.seek 25.00ml @09:59:37.65 Vacuum too high -- waiting up to 40s @09:59:37.92 Intake @36.4psia, vacuum from 20.0 to 24.0psi @09:59:37.94 SS.reconfigure forSampling @09:59:37.97 SS.seek 25.00ml @09:59:38.83 Vacuum too high -- waiting up to 40s @09:59:39.32 Intake @36.6psia, vacuum from 20.0 to 24.0psi @09:59:39.34 SS.reconfigure forSampling @09:59:39.37 SS.seek 25.00ml @09:59:40.62 Vacuum too high -- waiting up to 40s @09:59:40.89 Intake @36.7psia, vacuum from 20.0 to 24.0psi @09:59:40.91 SS.reconfigure forSampling @09:59:40.94 SS.seek 25.00ml @09:59:41.93 Vacuum too high -- waiting up to 40s @09:59:42.20 Intake @36.8psia, vacuum from 20.0 to 24.0psi @09:59:42.22 SS.reconfigure forSampling @09:59:42.25 SS.seek 25.00ml @09:59:43.15 Vacuum too high -- waiting up to 40s @09:59:43.42 Intake @36.7psia, vacuum from 20.0 to 24.0psi @09:59:43.44 SS.reconfigure forSampling @09:59:43.47 SS.seek 25.00ml @09:59:44.43 Vacuum too high -- waiting up to 40s @09:59:44.70 Intake @36.6psia, vacuum from 20.0 to 24.0psi @09:59:44.72 SS.reconfigure forSampling @09:59:44.75 SS.seek 25.00ml @09:59:45.68 Vacuum too high -- waiting up to 40s @09:59:46.18 SS.seek 25.00ml @09:59:47.40 Vacuum too high -- waiting up to 40s @09:59:47.67 Intake @36.4psia, vacuum from 20.0 to 24.0psi @09:59:47.69 SS.reconfigure forSampling @09:59:47.72 SS.seek 25.00ml @09:59:48.55 Vacuum too high -- waiting up to 40s @09:59:49.05 Intake @36.3psia, vacuum from 20.0 to 24.0psi @09:59:49.06 SS.reconfigure forSampling @09:59:49.09 SS.seek 25.00ml @09:59:50.30 Vacuum too high -- waiting up to 40s @09:59:50.80 Intake @36.1psia, vacuum from 20.0 to 24.0psi @09:59:50.81 SS.reconfigure forSampling @09:59:50.84 SS.seek 25.00ml @09:59:52.18 Vacuum too high -- waiting up to 40s @09:59:52.67 Intake @36.3psia, vacuum from 20.0 to 24.0psi @09:59:52.68 SS.reconfigure forSampling @09:59:52.72 SS.seek 25.00ml @09:59:54.02 Vacuum too high -- waiting up to 40s @09:59:54.51 Intake @36.8psia, vacuum from 20.0 to 24.0psi @09:59:54.52 SS.reconfigure forSampling @09:59:54.56 SS.seek 25.00ml @09:59:55.71 Vacuum too high -- waiting up to 40s @09:59:55.98 Intake @36.4psia, vacuum from 20.0 to 24.0psi @09:59:55.99 SS.reconfigure forSampling @09:59:56.03 SS.seek 25.00ml @09:59:56.96 Vacuum too high -- waiting up to 40s @09:59:58.03 Intake @35.8psia, vacuum from 20.0 to 24.0psi @09:59:58.04 SS.reconfigure forSampling @09:59:58.08 SS.seek 25.00ml @09:59:59.80 Vacuum too high -- waiting up to 40s @10:00:00.29 Intake @36.3psia, vacuum from 20.0 to 24.0psi @10:00:00.31 SS.reconfigure forSampling @10:00:00.34 SS.seek 25.00ml @10:00:01.49 Vacuum too high -- waiting up to 40s @10:00:01.98 Intake @36.3psia, vacuum from 20.0 to 24.0psi @10:00:02.00 SS.reconfigure forSampling @10:00:02.03 SS.seek 25.00ml @10:00:03.15 Vacuum too high -- waiting up to 40s @10:00:03.42 Intake @36.1psia, vacuum from 20.0 to 24.0psi @10:00:03.44 SS.reconfigure forSampling @10:00:03.47 SS.seek 25.00ml @10:00:04.43 Vacuum too high -- waiting up to 40s @10:00:04.95 Intake @36.5psia, vacuum from 20.0 to 24.0psi @10:00:04.96 SS.reconfigure forSampling @10:00:05.00 SS.seek 25.00ml @10:00:06.21 Vacuum too high -- waiting up to 40s @10:00:06.70 Intake @37.3psia, vacuum from 20.0 to 24.0psi @10:00:06.72 SS.reconfigure forSampling @10:00:06.75 SS.seek 25.00ml @10:00:07.96 Vacuum too high -- waiting up to 40s @10:00:08.45 Intake @36.6psia, vacuum from 20.0 to 24.0psi @10:00:08.47 SS.reconfigure forSampling @10:00:08.50 SS.seek 25.00ml @10:00:09.74 Vacuum too high -- waiting up to 40s @10:00:10.23 Intake @36.5psia, vacuum from 20.0 to 24.0psi @10:00:10.25 SS.reconfigure forSampling @10:00:10.28 SS.seek 25.00ml @10:00:11.52 Vacuum too high -- waiting up to 40s @10:00:12.01 Intake @36.8psia, vacuum from 20.0 to 24.0psi @10:00:12.03 SS.reconfigure forSampling @10:00:12.06 SS.seek 25.00ml @10:00:12.31 Stroke completed -- waiting for pressure to equalize ... @10:00:14.72 Intake.select :close @10:00:14.86 SSV.dial :exhaust @10:00:15.64 Exhaust.select :open @10:00:15.74 SS.reconfigure SSexhale @10:00:15.78 SS.seek 0.00ml @10:00:24.39 Exhaust.select :close @10:00:24.49 SSV.dial CBV @10:00:24.51 CBV.dial SSV @10:00:25.31 Allowing 185s for 25.0ml of remaining 650.0ml @10:00:25.32 Intake.select :open @10:00:27.46 Intake @37.4psia, vacuum from 20.0 to 24.0psi @10:00:27.48 SS.reconfigure forSampling @10:00:27.51 SS.seek 25.00ml @10:00:27.88 delay 30 seconds @10:00:29.39 Vacuum too high -- waiting up to 40s @10:00:29.66 Intake @36.4psia, vacuum from 20.0 to 24.0psi @10:00:29.68 SS.reconfigure forSampling @10:00:29.71 SS.seek 25.00ml @10:00:30.64 Vacuum too high -- waiting up to 40s @10:00:31.13 Intake @36.1psia, vacuum from 20.0 to 24.0psi @10:00:31.15 SS.reconfigure forSampling @10:00:31.18 SS.seek 25.00ml @10:00:32.43 Vacuum too high -- waiting up to 40s @10:00:32.93 SS.seek 25.00ml @10:00:34.11 Vacuum too high -- waiting up to 40s @10:00:34.60 Intake @36.7psia, vacuum from 20.0 to 24.0psi @10:00:34.62 SS.reconfigure forSampling @10:00:34.65 SS.seek 25.00ml @10:00:35.99 Vacuum too high -- waiting up to 40s @10:00:36.26 Intake @36.8psia, vacuum from 20.0 to 24.0psi @10:00:36.28 SS.reconfigure forSampling @10:00:36.31 SS.seek 25.00ml @10:00:37.21 Vacuum too high -- waiting up to 40s @10:00:37.49 Intake @36.7psia, vacuum from 20.0 to 24.0psi @10:00:37.50 SS.reconfigure forSampling @10:00:37.53 SS.seek 25.00ml @10:00:38.46 Vacuum too high -- waiting up to 40s @10:00:38.95 Intake @37.0psia, vacuum from 20.0 to 24.0psi @10:00:38.97 SS.reconfigure forSampling @10:00:39.00 SS.seek 25.00ml @10:00:40.21 Vacuum too high -- waiting up to 40s @10:00:41.07 SS.seek 25.00ml @10:00:42.55 Vacuum too high -- waiting up to 40s @10:00:43.05 Intake @36.5psia, vacuum from 20.0 to 24.0psi @10:00:43.06 SS.reconfigure forSampling @10:00:43.09 SS.seek 25.00ml @10:00:44.24 Vacuum too high -- waiting up to 40s @10:00:44.74 Intake @36.6psia, vacuum from 20.0 to 24.0psi @10:00:44.76 SS.reconfigure forSampling @10:00:44.79 SS.seek 25.00ml @10:00:46.14 Vacuum too high -- waiting up to 40s @10:00:46.63 Intake @37.0psia, vacuum from 20.0 to 24.0psi @10:00:46.65 SS.reconfigure forSampling @10:00:46.68 SS.seek 25.00ml @10:00:47.92 Vacuum too high -- waiting up to 40s @10:00:48.41 Intake @36.9psia, vacuum from 20.0 to 24.0psi @10:00:48.43 SS.reconfigure forSampling @10:00:48.46 SS.seek 25.00ml @10:00:49.64 Vacuum too high -- waiting up to 40s @10:00:50.14 Intake @36.5psia, vacuum from 20.0 to 24.0psi @10:00:50.16 SS.reconfigure forSampling @10:00:50.19 SS.seek 25.00ml @10:00:51.39 Vacuum too high -- waiting up to 40s @10:00:51.88 Intake @36.9psia, vacuum from 20.0 to 24.0psi @10:00:51.90 SS.reconfigure forSampling @10:00:51.93 SS.seek 25.00ml @10:00:53.11 Vacuum too high -- waiting up to 40s @10:00:53.60 Intake @36.5psia, vacuum from 20.0 to 24.0psi @10:00:53.62 SS.reconfigure forSampling @10:00:53.65 SS.seek 25.00ml @10:00:54.83 Vacuum too high -- waiting up to 40s @10:00:55.32 Intake @36.2psia, vacuum from 20.0 to 24.0psi @10:00:55.34 SS.reconfigure forSampling @10:00:55.37 SS.seek 25.00ml @10:00:56.64 Vacuum too high -- waiting up to 40s @10:00:57.13 Intake @36.8psia, vacuum from 20.0 to 24.0psi @10:00:57.15 SS.reconfigure forSampling @10:00:57.18 SS.seek 25.00ml @10:00:57.89 ATV.dial :extSrc @10:00:58.33 Vacuum too high -- waiting up to 40s @10:00:58.42 delay 3 seconds @10:00:58.82 Intake @37.1psia, vacuum from 20.0 to 24.0psi @10:00:58.84 SS.reconfigure forSampling @10:00:58.87 SS.seek 25.00ml @10:00:59.99 Vacuum too high -- waiting up to 40s @10:01:00.27 Intake @36.7psia, vacuum from 20.0 to 24.0psi @10:01:00.28 SS.reconfigure forSampling @10:01:00.31 SS.seek 25.00ml @10:01:01.24 Vacuum too high -- waiting up to 40s @10:01:01.46 ATV.dial :ElutionWater @10:01:01.55 Intake @36.4psia, vacuum from 20.0 to 24.0psi @10:01:01.56 SS.reconfigure forSampling @10:01:01.59 SS.seek 25.00ml @10:01:02.47 AS.seek 0.120ml @10:01:02.55 Vacuum too high -- waiting up to 40s @10:01:03.05 SS.seek 25.00ml @10:01:04.14 Vacuum too high -- waiting up to 40s @10:01:04.64 Intake @36.7psia, vacuum from 20.0 to 24.0psi @10:01:04.65 SS.reconfigure forSampling @10:01:04.68 SS.seek 25.00ml @10:01:05.86 Vacuum too high -- waiting up to 40s @10:01:06.35 Intake @37.0psia, vacuum from 20.0 to 24.0psi @10:01:06.37 SS.reconfigure forSampling @10:01:06.40 SS.seek 25.00ml @10:01:07.52 Vacuum too high -- waiting up to 40s @10:01:07.79 Intake @36.6psia, vacuum from 20.0 to 24.0psi @10:01:07.81 SS.reconfigure forSampling @10:01:07.84 SS.seek 25.00ml @10:01:08.81 Vacuum too high -- waiting up to 40s @10:01:08.83 ATV.dial :air3 @10:01:09.21 AS.seek 0.130ml @10:01:09.38 Intake @36.4psia, vacuum from 20.0 to 24.0psi @10:01:09.40 SS.reconfigure forSampling @10:01:09.43 SS.seek 25.00ml @10:01:10.70 Vacuum too high -- waiting up to 40s @10:01:11.56 delay 2 seconds @10:01:11.59 Intake @36.9psia, vacuum from 20.0 to 24.0psi @10:01:11.60 SS.reconfigure forSampling @10:01:11.63 SS.seek 25.00ml @10:01:13.08 Vacuum too high -- waiting up to 40s @10:01:13.57 Intake @37.3psia, vacuum from 20.0 to 24.0psi @10:01:13.59 SS.reconfigure forSampling @10:01:13.61 ATV.dial :bleach @10:01:13.64 SS.seek 25.00ml @10:01:14.02 AS.seek 0.230ml @10:01:14.83 Vacuum too high -- waiting up to 40s @10:01:15.32 Intake @36.8psia, vacuum from 20.0 to 24.0psi @10:01:15.34 SS.reconfigure forSampling @10:01:15.37 SS.seek 25.00ml @10:01:16.58 Vacuum too high -- waiting up to 40s @10:01:17.08 SS.seek 25.00ml @10:01:18.17 Vacuum too high -- waiting up to 40s @10:01:18.78 Intake @36.5psia, vacuum from 20.0 to 24.0psi @10:01:18.79 SS.reconfigure forSampling @10:01:18.82 SS.seek 25.00ml @10:01:20.02 Vacuum too high -- waiting up to 40s @10:01:20.51 Intake @36.6psia, vacuum from 20.0 to 24.0psi @10:01:20.53 SS.reconfigure forSampling @10:01:20.56 SS.seek 25.00ml @10:01:21.67 Vacuum too high -- waiting up to 40s @10:01:22.16 Intake @36.8psia, vacuum from 20.0 to 24.0psi @10:01:22.17 SS.reconfigure forSampling @10:01:22.21 SS.seek 25.00ml @10:01:23.45 Vacuum too high -- waiting up to 40s @10:01:23.95 SS.seek 25.00ml @10:01:25.05 Vacuum too high -- waiting up to 40s @10:01:25.54 Intake @36.8psia, vacuum from 20.0 to 24.0psi @10:01:25.56 SS.reconfigure forSampling @10:01:25.59 SS.seek 25.00ml @10:01:26.67 Vacuum too high -- waiting up to 40s @10:01:27.16 Intake @36.5psia, vacuum from 20.0 to 24.0psi @10:01:27.17 SS.reconfigure forSampling @10:01:27.21 SS.seek 25.00ml @10:01:28.30 Vacuum too high -- waiting up to 40s @10:01:28.57 Intake @36.6psia, vacuum from 20.0 to 24.0psi @10:01:28.59 SS.reconfigure forSampling @10:01:28.62 SS.seek 25.00ml @10:01:29.52 Vacuum too high -- waiting up to 40s @10:01:30.01 Intake @36.9psia, vacuum from 20.0 to 24.0psi @10:01:30.03 SS.reconfigure forSampling @10:01:30.06 SS.seek 25.00ml @10:01:31.11 Vacuum too high -- waiting up to 40s @10:01:31.60 Intake @36.8psia, vacuum from 20.0 to 24.0psi @10:01:31.62 SS.reconfigure forSampling @10:01:31.65 SS.seek 25.00ml @10:01:32.80 Vacuum too high -- waiting up to 40s @10:01:33.29 Intake @36.7psia, vacuum from 20.0 to 24.0psi @10:01:33.30 SS.reconfigure forSampling @10:01:33.34 SS.seek 25.00ml @10:01:34.42 Vacuum too high -- waiting up to 40s @10:01:34.74 delay 3 seconds @10:01:34.93 Intake @36.5psia, vacuum from 20.0 to 24.0psi @10:01:34.94 SS.reconfigure forSampling @10:01:34.97 SS.seek 25.00ml @10:01:36.05 Vacuum too high -- waiting up to 40s @10:01:36.54 Intake @37.0psia, vacuum from 20.0 to 24.0psi @10:01:36.55 SS.reconfigure forSampling @10:01:36.59 SS.seek 25.00ml @10:01:37.77 Vacuum too high -- waiting up to 40s @10:01:37.78 ATV.dial :air3 @10:01:38.24 delay 3 seconds @10:01:38.28 Intake @36.9psia, vacuum from 20.0 to 24.0psi @10:01:38.30 SS.reconfigure forSampling @10:01:38.33 SS.seek 25.00ml @10:01:39.42 Vacuum too high -- waiting up to 40s @10:01:39.91 Intake @36.7psia, vacuum from 20.0 to 24.0psi @10:01:39.93 SS.reconfigure forSampling @10:01:39.96 SS.seek 25.00ml @10:01:41.31 AS.seek 0.255ml @10:01:42.20 Vacuum too high -- waiting up to 40s @10:01:42.70 Intake @36.9psia, vacuum from 20.0 to 24.0psi @10:01:42.71 SS.reconfigure forSampling @10:01:42.74 SS.seek 25.00ml @10:01:43.98 Vacuum too high -- waiting up to 40s @10:01:44.47 Intake @37.0psia, vacuum from 20.0 to 24.0psi @10:01:44.49 SS.reconfigure forSampling @10:01:44.52 SS.seek 25.00ml @10:01:45.52 Vacuum too high -- waiting up to 40s @10:01:45.79 Intake @36.7psia, vacuum from 20.0 to 24.0psi @10:01:45.81 SS.reconfigure forSampling @10:01:45.84 SS.seek 25.00ml @10:01:46.68 Vacuum too high -- waiting up to 40s @10:01:46.70 delay 3 seconds @10:01:46.95 Intake @36.5psia, vacuum from 20.0 to 24.0psi @10:01:46.97 SS.reconfigure forSampling @10:01:47.00 SS.seek 25.00ml @10:01:47.92 Vacuum too high -- waiting up to 40s @10:01:48.41 Intake @36.9psia, vacuum from 20.0 to 24.0psi @10:01:48.43 SS.reconfigure forSampling @10:01:48.46 SS.seek 25.00ml @10:01:49.52 Vacuum too high -- waiting up to 40s @10:01:49.76 ATV.dial :bleach @10:01:50.01 Intake @37.0psia, vacuum from 20.0 to 24.0psi @10:01:50.03 SS.reconfigure forSampling @10:01:50.06 SS.seek 25.00ml @10:01:50.24 AS.seek 0.240ml @10:01:51.17 Vacuum too high -- waiting up to 40s @10:01:51.66 Intake @36.7psia, vacuum from 20.0 to 24.0psi @10:01:51.68 SS.reconfigure forSampling @10:01:51.71 SS.seek 25.00ml @10:01:52.70 Vacuum too high -- waiting up to 40s @10:01:53.20 SS.seek 25.00ml @10:01:53.52 delay 3 seconds @10:01:54.17 Vacuum too high -- waiting up to 40s @10:01:54.67 Intake @36.7psia, vacuum from 20.0 to 24.0psi @10:01:54.68 SS.reconfigure forSampling @10:01:54.71 SS.seek 25.00ml @10:01:55.86 Vacuum too high -- waiting up to 40s @10:01:56.35 Intake @36.8psia, vacuum from 20.0 to 24.0psi @10:01:56.36 SS.reconfigure forSampling @10:01:56.40 SS.seek 25.00ml @10:01:56.53 ATV.dial :ASV @10:01:57.44 ASV.dial :SPEdelivery @10:01:57.50 Vacuum too high -- waiting up to 40s @10:01:58.00 Intake @36.7psia, vacuum from 20.0 to 24.0psi @10:01:58.01 SS.reconfigure forSampling @10:01:58.05 SS.seek 25.00ml @10:01:58.20 AFV.select :carrierSolution @10:01:58.30 delay 2 seconds @10:01:59.14 Vacuum too high -- waiting up to 40s @10:01:59.63 Intake @36.8psia, vacuum from 20.0 to 24.0psi @10:01:59.64 SS.reconfigure forSampling @10:01:59.68 SS.seek 25.00ml @10:02:00.31 AS.reconfigure ASconfig @10:02:00.35 AS.seek 0.500ml @10:02:00.70 Vacuum too high -- waiting up to 40s @10:02:01.19 Intake @36.7psia, vacuum from 20.0 to 24.0psi @10:02:01.21 SS.reconfigure forSampling @10:02:01.24 SS.seek 25.00ml @10:02:02.36 Vacuum too high -- waiting up to 40s @10:02:02.85 Intake @36.3psia, vacuum from 20.0 to 24.0psi @10:02:02.87 SS.reconfigure forSampling @10:02:02.90 SS.seek 25.00ml @10:02:04.01 Vacuum too high -- waiting up to 40s @10:02:04.50 Intake @36.9psia, vacuum from 20.0 to 24.0psi @10:02:04.52 SS.reconfigure forSampling @10:02:04.55 SS.seek 25.00ml @10:02:05.55 Vacuum too high -- waiting up to 40s @10:02:06.04 Intake @37.1psia, vacuum from 20.0 to 24.0psi @10:02:06.06 SS.reconfigure forSampling @10:02:06.09 SS.seek 25.00ml @10:02:07.11 Vacuum too high -- waiting up to 40s @10:02:08.22 Intake @36.9psia, vacuum from 20.0 to 24.0psi @10:02:08.24 SS.reconfigure forSampling @10:02:08.27 SS.seek 25.00ml @10:02:09.73 Vacuum too high -- waiting up to 40s @10:02:10.01 Intake @36.5psia, vacuum from 20.0 to 24.0psi @10:02:10.03 SS.reconfigure forSampling @10:02:10.06 SS.seek 25.00ml @10:02:10.92 Vacuum too high -- waiting up to 40s @10:02:11.41 Intake @36.4psia, vacuum from 20.0 to 24.0psi @10:02:11.43 SS.reconfigure forSampling @10:02:11.46 SS.seek 25.00ml @10:02:12.51 Vacuum too high -- waiting up to 40s @10:02:13.02 Intake @36.4psia, vacuum from 20.0 to 24.0psi @10:02:13.03 SS.reconfigure forSampling @10:02:13.06 SS.seek 25.00ml @10:02:14.08 Vacuum too high -- waiting up to 40s @10:02:14.57 Intake @36.7psia, vacuum from 20.0 to 24.0psi @10:02:14.59 SS.reconfigure forSampling @10:02:14.62 SS.seek 25.00ml @10:02:15.61 Vacuum too high -- waiting up to 40s @10:02:15.86 delay 1 second @10:02:16.12 Intake @36.9psia, vacuum from 20.0 to 24.0psi @10:02:16.13 SS.reconfigure forSampling @10:02:16.16 SS.seek 25.00ml @10:02:16.87 AFV.select :holdingCoil @10:02:17.02 AS.reconfigure ASslow1 @10:02:17.05 AS.seek 0.170ml @10:02:17.20 Vacuum too high -- waiting up to 40s @10:02:17.70 Intake @37.2psia, vacuum from 20.0 to 24.0psi @10:02:17.71 SS.reconfigure forSampling @10:02:17.74 SS.seek 25.00ml @10:02:18.76 Vacuum too high -- waiting up to 40s @10:02:19.27 Intake @36.9psia, vacuum from 20.0 to 24.0psi @10:02:19.28 SS.reconfigure forSampling @10:02:19.31 SS.seek 25.00ml @10:02:20.33 Vacuum too high -- waiting up to 40s @10:02:20.83 SS.seek 25.00ml @10:02:21.76 Vacuum too high -- waiting up to 40s @10:02:22.25 Intake @36.5psia, vacuum from 20.0 to 24.0psi @10:02:22.27 SS.reconfigure forSampling @10:02:22.30 SS.seek 25.00ml @10:02:23.39 Vacuum too high -- waiting up to 40s @10:02:23.88 Intake @36.6psia, vacuum from 20.0 to 24.0psi @10:02:23.89 SS.reconfigure forSampling @10:02:23.93 SS.seek 25.00ml @10:02:24.95 Vacuum too high -- waiting up to 40s @10:02:25.44 Intake @36.9psia, vacuum from 20.0 to 24.0psi @10:02:25.46 SS.reconfigure forSampling @10:02:25.49 SS.seek 25.00ml @10:02:26.51 Vacuum too high -- waiting up to 40s @10:02:27.01 Intake @37.0psia, vacuum from 20.0 to 24.0psi @10:02:27.02 SS.reconfigure forSampling @10:02:27.05 SS.seek 25.00ml @10:02:28.01 Vacuum too high -- waiting up to 40s @10:02:28.50 Intake @36.7psia, vacuum from 20.0 to 24.0psi @10:02:28.51 SS.reconfigure forSampling @10:02:28.55 SS.seek 25.00ml @10:02:29.64 Vacuum too high -- waiting up to 40s @10:02:30.15 Intake @37.1psia, vacuum from 20.0 to 24.0psi @10:02:30.16 SS.reconfigure forSampling @10:02:30.19 SS.seek 25.00ml @10:02:31.20 Vacuum too high -- waiting up to 40s @10:02:31.69 Intake @37.1psia, vacuum from 20.0 to 24.0psi @10:02:31.70 SS.reconfigure forSampling @10:02:31.74 SS.seek 25.00ml @10:02:32.79 Vacuum too high -- waiting up to 40s @10:02:33.29 Intake @36.7psia, vacuum from 20.0 to 24.0psi @10:02:33.30 SS.reconfigure forSampling @10:02:33.33 SS.seek 25.00ml @10:02:34.29 Vacuum too high -- waiting up to 40s @10:02:34.78 Intake @36.4psia, vacuum from 20.0 to 24.0psi @10:02:34.80 SS.reconfigure forSampling @10:02:34.83 SS.seek 25.00ml @10:02:35.92 Vacuum too high -- waiting up to 40s @10:02:36.79 Intake @36.5psia, vacuum from 20.0 to 24.0psi @10:02:36.81 SS.reconfigure forSampling @10:02:36.84 SS.seek 25.00ml @10:02:38.26 Vacuum too high -- waiting up to 40s @10:02:38.76 Intake @36.9psia, vacuum from 20.0 to 24.0psi @10:02:38.77 SS.reconfigure forSampling @10:02:38.80 SS.seek 25.00ml @10:02:39.76 Vacuum too high -- waiting up to 40s @10:02:40.16 Intake @37.1psia, vacuum from 20.0 to 24.0psi @10:02:40.17 SS.reconfigure forSampling @10:02:40.21 SS.seek 25.00ml @10:02:41.11 Vacuum too high -- waiting up to 40s @10:02:41.39 Intake @36.6psia, vacuum from 20.0 to 24.0psi @10:02:41.40 SS.reconfigure forSampling @10:02:41.43 SS.seek 25.00ml @10:02:42.20 Vacuum too high -- waiting up to 40s @10:02:42.71 Intake @36.8psia, vacuum from 20.0 to 24.0psi @10:02:42.72 SS.reconfigure forSampling @10:02:42.76 SS.seek 25.00ml @10:02:43.79 Vacuum too high -- waiting up to 40s @10:02:44.28 Intake @36.4psia, vacuum from 20.0 to 24.0psi @10:02:44.30 SS.reconfigure forSampling @10:02:44.33 SS.seek 25.00ml @10:02:45.36 Vacuum too high -- waiting up to 40s @10:02:45.86 Intake @36.7psia, vacuum from 20.0 to 24.0psi @10:02:45.87 SS.reconfigure forSampling @10:02:45.90 SS.seek 25.00ml @10:02:46.92 Vacuum too high -- waiting up to 40s @10:02:47.43 Intake @36.6psia, vacuum from 20.0 to 24.0psi @10:02:47.44 SS.reconfigure forSampling @10:02:47.47 SS.seek 25.00ml @10:02:48.61 Vacuum too high -- waiting up to 40s @10:02:49.13 SS.seek 25.00ml @10:02:50.11 Vacuum too high -- waiting up to 40s @10:02:50.61 Intake @36.8psia, vacuum from 20.0 to 24.0psi @10:02:50.62 SS.reconfigure forSampling @10:02:50.65 SS.seek 25.00ml @10:02:51.61 Vacuum too high -- waiting up to 40s @10:02:52.12 Intake @36.8psia, vacuum from 20.0 to 24.0psi @10:02:52.13 SS.reconfigure forSampling @10:02:52.16 SS.seek 25.00ml @10:02:53.17 Vacuum too high -- waiting up to 40s @10:02:53.46 Intake @36.0psia, vacuum from 20.0 to 24.0psi @10:02:53.47 SS.reconfigure forSampling @10:02:53.51 SS.seek 25.00ml @10:02:54.39 Vacuum too high -- waiting up to 40s @10:02:54.90 Intake @36.2psia, vacuum from 20.0 to 24.0psi @10:02:54.91 SS.reconfigure forSampling @10:02:54.94 SS.seek 25.00ml @10:02:55.92 Vacuum too high -- waiting up to 40s @10:02:56.44 Intake @36.4psia, vacuum from 20.0 to 24.0psi @10:02:56.45 SS.reconfigure forSampling @10:02:56.48 SS.seek 25.00ml @10:02:57.51 Vacuum too high -- waiting up to 40s @10:02:58.02 Intake @37.1psia, vacuum from 20.0 to 24.0psi @10:02:58.04 SS.reconfigure forSampling @10:02:58.07 SS.seek 25.00ml @10:02:59.07 Vacuum too high -- waiting up to 40s @10:02:59.58 Intake @37.2psia, vacuum from 20.0 to 24.0psi @10:02:59.59 SS.reconfigure forSampling @10:02:59.63 SS.seek 25.00ml @10:03:00.73 Vacuum too high -- waiting up to 40s @10:03:01.23 Intake @36.5psia, vacuum from 20.0 to 24.0psi @10:03:01.24 SS.reconfigure forSampling @10:03:01.28 SS.seek 25.00ml @10:03:02.29 Vacuum too high -- waiting up to 40s @10:03:02.78 Intake @37.0psia, vacuum from 20.0 to 24.0psi @10:03:02.79 SS.reconfigure forSampling @10:03:02.83 SS.seek 25.00ml @10:03:03.82 Vacuum too high -- waiting up to 40s @10:03:04.31 Intake @37.2psia, vacuum from 20.0 to 24.0psi @10:03:04.33 SS.reconfigure forSampling @10:03:04.36 SS.seek 25.00ml @10:03:05.96 Vacuum too high -- waiting up to 40s @10:03:06.23 Intake @36.8psia, vacuum from 20.0 to 24.0psi @10:03:06.24 SS.reconfigure forSampling @10:03:06.27 SS.seek 25.00ml @10:03:07.64 Vacuum too high -- waiting up to 40s @10:03:08.17 Intake @36.5psia, vacuum from 20.0 to 24.0psi @10:03:08.19 SS.reconfigure forSampling @10:03:08.22 SS.seek 25.00ml @10:03:09.26 Vacuum too high -- waiting up to 40s @10:03:09.77 Intake @36.7psia, vacuum from 20.0 to 24.0psi @10:03:09.78 SS.reconfigure forSampling @10:03:09.81 SS.seek 25.00ml @10:03:10.70 Vacuum too high -- waiting up to 40s @10:03:11.22 Intake @36.9psia, vacuum from 20.0 to 24.0psi @10:03:11.23 SS.reconfigure forSampling @10:03:11.27 SS.seek 25.00ml @10:03:12.27 Vacuum too high -- waiting up to 40s @10:03:12.78 Intake @37.0psia, vacuum from 20.0 to 24.0psi @10:03:12.79 SS.reconfigure forSampling @10:03:12.82 SS.seek 25.00ml @10:03:13.76 Vacuum too high -- waiting up to 40s @10:03:14.26 Intake @36.9psia, vacuum from 20.0 to 24.0psi @10:03:14.28 SS.reconfigure forSampling @10:03:14.31 SS.seek 25.00ml @10:03:15.32 Vacuum too high -- waiting up to 40s @10:03:15.81 Intake @37.1psia, vacuum from 20.0 to 24.0psi @10:03:15.83 SS.reconfigure forSampling @10:03:15.86 SS.seek 25.00ml @10:03:16.95 Vacuum too high -- waiting up to 40s @10:03:17.44 Intake @36.8psia, vacuum from 20.0 to 24.0psi @10:03:17.46 SS.reconfigure forSampling @10:03:17.49 SS.seek 25.00ml @10:03:18.39 Vacuum too high -- waiting up to 40s @10:03:18.89 Intake @36.4psia, vacuum from 20.0 to 24.0psi @10:03:18.90 SS.reconfigure forSampling @10:03:18.93 SS.seek 25.00ml @10:03:19.98 Vacuum too high -- waiting up to 40s @10:03:20.47 Intake @36.6psia, vacuum from 20.0 to 24.0psi @10:03:20.49 SS.reconfigure forSampling @10:03:20.52 SS.seek 25.00ml @10:03:21.48 Vacuum too high -- waiting up to 40s @10:03:21.97 Intake @36.6psia, vacuum from 20.0 to 24.0psi @10:03:21.99 SS.reconfigure forSampling @10:03:22.03 SS.seek 25.00ml @10:03:23.01 Vacuum too high -- waiting up to 40s @10:03:23.56 SS.seek 25.00ml @10:03:24.57 Vacuum too high -- waiting up to 40s @10:03:24.63 SPE.hold 2:46:40,95.0C @10:03:24.65 delay 3:00 @10:03:25.06 Intake @36.4psia, vacuum from 20.0 to 24.0psi @10:03:25.08 SS.reconfigure forSampling @10:03:25.11 SS.seek 25.00ml @10:03:26.07 Vacuum too high -- waiting up to 40s @10:03:26.57 Intake @36.3psia, vacuum from 20.0 to 24.0psi @10:03:26.59 SS.reconfigure forSampling @10:03:26.62 SS.seek 25.00ml @10:03:27.60 Vacuum too high -- waiting up to 40s @10:03:28.09 Intake @36.1psia, vacuum from 20.0 to 24.0psi @10:03:28.11 SS.reconfigure forSampling @10:03:28.14 SS.seek 25.00ml @10:03:29.14 Vacuum too high -- waiting up to 40s @10:03:29.63 Intake @35.8psia, vacuum from 20.0 to 24.0psi @10:03:29.65 SS.reconfigure forSampling @10:03:29.68 SS.seek 25.00ml @10:03:30.32 SS.reconfigure SSconfig @10:03:30.35 SS.hold @10:03:30.48 Intake.select :close @10:03:30.64 ShallowSampler::Clogged in WCR -- progress too slow after 371.9ml @10:03:30.65 SSV.dial :exhaust @10:03:31.43 Exhaust.select :open @10:03:31.54 SS.reconfigure SSexhale @10:03:31.58 SS.seek 0.00ml @10:03:39.86 Exhaust.select :close @10:03:39.97 Sampled 371.9ml @10:03:39.98 collectSample: Complete @10:03:40.57 Drying puck before fixing for 04:00.00 CBV.dial :waste2b @10:03:41.36 delay 1:00 @10:03:42.96 Sleepy.power :sampler,:OFF @10:04:41.36 CSV.dial :air @10:04:41.81 CS.reconfigure CSfast @10:04:41.84 CS.seek 10.000ml @10:05:06.35 CBV.dial between CSV and waste2b @10:05:06.67 Pumping wcr puck from tube 2 up to 55psi... @10:05:06.72 CBV.dial SSV @10:05:08.17 CBV.dial between CSV and waste2b @10:05:09.13 CSV.dial between air and CTV @10:05:09.52 CS.seek 6.464ml @10:05:18.64 CTV.dial CSV,avoiding => air @10:05:18.66 CSV.dial CTV @10:05:19.45 CS.reconfigure CSpressurize @10:05:19.48 CS.seek 0.000ml @10:05:36.44 CSV.dial :air @10:05:36.98 CS.reconfigure CSfast @10:05:37.01 CS.seek 10.000ml @10:06:01.26 CSV.dial between air and CTV @10:06:01.65 CS.seek 2.020ml @10:06:21.26 CTV.dial CSV,avoiding => air @10:06:21.27 CSV.dial CTV @10:06:21.58 CS.reconfigure CSpressurize @10:06:21.61 CS.seek 0.000ml @10:06:22.18 CBV.dial :waste2b @10:06:22.50 CS.reconfigure CSconfig @10:06:22.53 CS.seek empty @10:06:24.66 AFV.select :carrierSolution @10:06:24.75 delay 2 seconds @10:06:26.76 AS.reconfigure ASconfig @10:06:26.79 AS.seek 1.000ml @10:06:28.35 delay 1:00 @10:07:15.42 delay 1 second @10:07:16.43 AFV.select :holdingCoil @10:07:16.52 delay 1 second @10:07:17.53 ASV.dial :wasteD @10:07:18.40 AS.seek 0.000ml @10:07:28.37 CTV.dial :air,avoiding => intake @10:07:29.68 Fix #1 of 2 (with rnal) for 02:00.00 @10:07:29.69 CS Pulling 2ml of rnal CSV.dial CSR @10:07:30.34 CSR.select :rnal @10:07:30.44 CS.seek 2.000ml @10:07:36.29 delay 5 seconds @10:07:41.30 CSR.select :air @10:07:41.39 CS.reconfigure CSfast @10:07:41.43 CS.seek 10.000ml @10:08:00.70 CBV.dial between CSV and waste2b @10:08:01.03 Pumping wcr puck from tube 2 up to 55psi... @10:08:01.07 CBV.dial SSV @10:08:02.50 CBV.dial between CSV and waste2b @10:08:03.45 CSV.dial between air and CTV @10:08:04.03 CS.seek 6.432ml @10:08:13.08 CTV.dial CSV,avoiding => air @10:08:13.10 CSV.dial CTV @10:08:14.42 CS.reconfigure CSpressurize @10:08:14.45 CS.seek 0.000ml @10:08:17.00 Priming Eluate @10:08:17.01 AFV.select :carrierSolution @10:08:17.10 delay 2 seconds @10:08:19.11 AS.seek 0.500ml @10:08:30.69 delay 2:00 @10:08:48.55 delay 1 second @10:08:49.56 AFV.select :holdingCoil @10:08:49.65 ATV.dial ASV @10:08:49.66 ASV.dial :SPErecovery @10:08:50.31 Swish #1 of 2 @10:08:50.36 AS.reconfigure ASslow0 @10:08:50.40 AS.seek 0.600ml @10:09:00.92 AS.seek 0.500ml @10:09:11.42 Swish #2 of 2 @10:09:11.48 AS.seek 0.600ml @10:09:22.03 AS.seek 0.500ml @10:09:32.54 delay 3 seconds @10:09:35.60 AS.reconfigure ASslow1 @10:09:35.63 AS.seek 0.676ml @10:10:11.79 delay 3 seconds @10:10:14.80 Eluate primed @10:10:14.81 ATV.dial :waste2 @10:10:15.33 AS.reconfigure ASnorm @10:10:15.36 AS.seek 0.000ml @10:10:30.70 CBV.dial :waste2b @10:10:31.03 CS.reconfigure CSconfig @10:10:31.06 CS.seek empty @10:10:32.22 delay 1:00 @10:10:50.21 SPE.coast @10:10:50.29 ASV.dial :SPErecovery @10:10:50.30 Setting up PCR for HF183em -- writing /var/log/esp/HF183em14feb1510h374ml.pcr @10:10:50.31 Building PCR Slug from ASV with MM2 mix, PP4 primer @10:10:50.32 Priming with carrier @10:10:50.33 ATV.dial ARV @10:10:51.03 ARV.dial :carrier @10:10:51.33 AS.seek 0.500ml @10:11:17.21 AFV.select :carrierSolution @10:11:17.30 delay 2 seconds @10:11:19.31 AS.reconfigure ASconfig @10:11:19.34 AS.seek 1.000ml @10:11:32.23 CTV.dial :air,avoiding => intake @10:11:34.03 Fix #2 of 2 (with rnal) for 02:00.00 @10:11:34.04 CS Pulling 2ml of rnal CSV.dial CSR @10:11:34.47 CSR.select :rnal @10:11:34.57 CS.seek 2.000ml @10:11:40.47 delay 5 seconds @10:11:45.48 CSR.select :air @10:11:45.57 CS.reconfigure CSfast @10:11:45.61 CS.seek 10.000ml @10:11:48.79 delay 1 second @10:11:49.80 AFV.select :holdingCoil @10:11:49.90 delay 1 second @10:11:50.91 ATV.dial :waste @10:11:52.19 AS.reconfigure ASnorm @10:11:52.22 AS.seek empty @10:12:04.56 CBV.dial between CSV and waste2b @10:12:04.88 Pumping wcr puck from tube 2 up to 55psi... @10:12:04.93 CBV.dial SSV @10:12:06.37 CBV.dial between CSV and waste2b @10:12:07.31 CSV.dial between air and CTV @10:12:07.96 CS.seek 6.388ml @10:12:16.98 CTV.dial CSV,avoiding => air @10:12:17.00 CSV.dial CTV @10:12:18.30 CS.reconfigure CSpressurize @10:12:18.33 CS.seek 0.000ml @10:12:34.46 delay 2:00 @10:12:43.69 AFV.select :carrierSolution @10:12:43.80 delay 2 seconds @10:12:45.81 AS.reconfigure ASconfig @10:12:45.85 AS.seek 0.500ml @10:13:15.35 delay 1 second @10:13:16.36 AFV.select :holdingCoil @10:13:16.45 Building Slug @10:13:16.46 ATV.dial :ARV @10:13:17.72 delay 2 seconds @10:13:19.73 ARV.dial :air1 @10:13:20.07 delay 2 seconds @10:13:22.13 AS.reconfigure ASslow2 @10:13:22.16 AS.seek 0.506ml @10:13:28.61 delay 2 seconds @10:13:30.62 ARV.dial :MM2 @10:13:31.93 delay 2 seconds @10:13:33.94 Adding MM2 @10:13:34.00 AS.seek 0.524ml @10:13:52.77 delay 25 seconds @10:14:17.78 ARV.dial :PP4 @10:14:18.11 Adding PP4 @10:14:18.12 delay 2 seconds @10:14:20.18 AS.seek 0.530ml @10:14:26.63 delay 10 seconds @10:14:34.47 CBV.dial :waste2b @10:14:34.80 CS.reconfigure CSconfig @10:14:34.83 CS.seek empty @10:14:35.99 delay 1:00 @10:14:36.64 ARV.dial :air3 @10:14:36.98 adding ARV air @10:14:36.99 delay 2 seconds @10:14:39.04 AS.seek 0.536ml @10:14:45.53 delay 5 seconds @10:14:50.54 ARV.dial :carrier @10:14:51.57 delay 7 seconds @10:14:58.63 AS.seek 0.594ml @10:15:36.00 CTV.dial :air,avoiding => intake @10:15:37.32 Storing wcr puck in Collection Clamp to tube 1 @10:15:37.45 SC.reconfigure SCtight @10:15:37.48 SC.seek 1 @10:15:39.69 SC.reconfigure SCloose @10:15:39.87 Elbow.reconfigure TightElbow @10:15:39.91 Elbow.seek 1 @10:15:41.53 Forearm.reconfigure TightForearm @10:15:41.56 Forearm.seek 1 + 800 counts @10:15:42.67 SE.reconfigure SEpick @10:15:42.72 SE.seek beamBreak @10:15:44.99 SE.configure SEconfig @10:15:45.11 Elbow.reconfigure LimpElbow @10:15:45.15 Forearm.reconfigure LooseForearm @10:15:45.19 Forearm.seek 1 @10:15:45.43 Elbow.hold @10:15:45.49 Elbow.reconfigure TightElbow @10:15:45.57 SE.seek 7.05 Pucks below Top Plate @10:15:45.88 Forearm.coast @10:15:45.91 Forearm.seek 1 + 338 counts @10:15:46.14 Forearm.reconfigure TightForearm @10:15:46.18 Forearm.seek 1 + 800 counts @10:15:46.42 SE.seek 8.05 Pucks below Top Plate @10:15:46.96 Forearm.reconfigure LooseForearm @10:15:46.99 Forearm.seek retract @10:15:48.49 Elbow.seek CC @10:15:50.04 Elbow.reconfigure LooseElbow @10:15:50.12 Forearm.seek CC @10:15:51.09 CC.seek sealed @10:15:52.02 Hand.grip @10:15:52.70 CC.seek open @10:15:56.07 Forearm.seek retract @10:15:57.02 Elbow.reconfigure TightElbow @10:15:57.05 Elbow.seek 1 @10:15:58.14 delay 5 seconds @10:15:58.64 Elbow.reconfigure LimpElbow @10:15:58.69 Forearm.seek 1 @10:15:59.68 Elbow.hold @10:15:59.74 Elbow.reconfigure TightElbow @10:15:59.78 Hand.release @10:16:00.67 Forearm.coast @10:16:00.70 Forearm.seek 1 + 339 counts @10:16:00.93 Forearm.reconfigure TightForearm @10:16:00.96 Forearm.seek 1 + 800 counts @10:16:01.23 SE.seek down @10:16:02.34 Forearm.reconfigure LooseForearm @10:16:02.37 Forearm.seek retract @10:16:03.15 ATV.dial :ASV @10:16:03.19 SE.coast @10:16:03.29 SC.coast @10:16:03.32 Starting finalPurge @10:16:03.33 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @10:16:03.48 Elbow.seek FlushPuck::Garage @10:16:03.54 delay 2 seconds @10:16:05.26
Elbow.reconfigure LooseElbow @10:16:05.34 Forearm.seek garage @10:16:05.59 AS.seek 0.600ml @10:16:06.36
Hand.grip @10:16:07.17 Forearm.seek retract @10:16:08.19 Elbow.reconfigure TightElbow @10:16:08.23 Elbow.seek CC @10:16:10.99 Elbow.reconfigure LooseElbow @10:16:11.07 Forearm.reconfigure TightForearm @10:16:11.10 Forearm.seek CC @10:16:11.86 delay 4 seconds @10:16:12.23
CC.seek closed @10:16:15.88 ATV.dial ARV @10:16:16.05
Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 2.01 @10:16:16.10 Hand.release @10:16:16.32 delay 2 seconds @10:16:16.87
Forearm.reconfigure LooseForearm @10:16:16.90 Forearm.seek retract @10:16:17.82 Flushing the Collection Valve::Manifold @10:16:17.92 CS Pulling 5ml of flush CSV.dial CSR @10:16:18.32 ARV.dial :carrier @10:16:18.33 delay 2 seconds @10:16:18.36
CSR.select :flush @10:16:18.45 CS.seek 5.000ml @10:16:20.39 AS.seek 0.650ml @10:16:31.92
delay 5 seconds @10:16:36.93 CSR.select :air @10:16:37.07 CS.reconfigure CSfast @10:16:37.10 CS.seek 7.500ml @10:16:43.61 CSV.dial :waste @10:16:45.73 CS.reconfigure CSconfig @10:16:45.76 CS.seek 0.000ml @10:17:05.58 Flushing lower manifold's waste1 port @10:17:05.67 CSV.dial :flush @10:17:06.20 CS.reconfigure CSfast @10:17:06.23 CS.seek 5.000ml @10:17:12.17 delay 4 seconds @10:17:16.18 ATV.dial PCR @10:17:16.70 ASV.dial :waste1 @10:17:17.39 ARV.dial :air1 @10:17:17.73 Positioning Slug by 0.324ml @10:17:17.78 AS.seek 0.326ml @10:17:18.30
delay 5 seconds @10:17:23.31 CSV.dial :air @10:17:23.78 CS.seek 7.000ml @10:17:29.23 CSV.dial CTV @10:17:29.25
CTV.dial CSV @10:17:30.59 CBV.dial :waste1 @10:17:31.35 CS.seek 0.000ml @10:17:47.89 Flushing lower manifold's waste2b port @10:17:47.99 CSV.dial :flush @10:17:48.79 CS.seek 5.000ml @10:18:00.95 delay 5 seconds @10:18:05.96 CSV.dial :air @10:18:06.53 CS.seek 7.000ml @10:18:11.86 CSV.dial CTV @10:18:11.88
CTV.dial CSV @10:18:12.31 CBV.dial :waste2b @10:18:13.05 CS.seek 0.000ml @10:18:29.61 Flushing upper manifold @10:18:29.70 CSV.dial :flush @10:18:30.49 CS.seek 5.000ml @10:18:42.54 delay 5 seconds @10:18:47.55 CSV.dial :air @10:18:48.09 CS.seek 6.999ml @10:18:53.42 CSV.dial CBV @10:18:53.44
CBV.dial CSV @10:18:54.56 CTV.dial :air @10:18:55.82 CS.seek 0.000ml @10:19:12.42 delay 7 seconds @10:19:19.43 CS.seek 3.000ml @10:19:27.01 CSV.dial :waste @10:19:27.54 CS.seek 0.000ml @10:19:35.46 CSV.dial :flush @10:19:35.89 CS.seek 3.000ml @10:19:43.45 delay 5 seconds @10:19:48.46 CSV.dial :air @10:19:48.95 CS.seek 5.000ml @10:19:54.38 CSV.dial :air @10:19:54.40 CS.seek 0.000ml @10:20:06.51 delay 7 seconds @10:20:13.52 CS.seek 3.000ml @10:20:21.13 CSV.dial :waste @10:20:21.94 CS.seek 0.000ml @10:20:29.73 Disinfecting external Intake with mfbkill @10:20:29.82 CSV.dial CSR @10:20:31.07 CSR.select :mfbkill @10:20:31.17 CS.seek 8.000ml @10:20:49.91 delay 5 seconds @10:20:54.92 CSR.select :air @10:20:55.01 CS.seek 10.000ml @10:21:00.44 CSV.dial CBV @10:21:00.47
CBV.dial CSV @10:21:01.52 CTV.dial :intake @10:21:02.24 Intake.select :open @10:21:02.33 CS.seek 2.000ml @10:21:21.41 Intake.select :close @10:21:21.50 CTV.dial :air @10:21:22.26 CS.reconfigure CSconfig @10:21:22.30 CS.seek 0.000ml @10:21:28.19 Flushing lower manifold's waste1 port @10:21:28.20 CS Pulling 4ml of mfbkill @10:21:28.21 CSV.dial CSR @10:21:29.26 CSR.select :mfbkill @10:21:29.36 CS.seek 4.000ml @10:21:40.47 delay 5 seconds @10:21:45.48 CSR.select :air @10:21:45.57 CS.reconfigure CSfast @10:21:45.61 CS.seek 10.000ml @10:21:59.89 CSV.dial CTV @10:21:59.91
CTV.dial CSV @10:22:01.24 CBV.dial :waste1 @10:22:01.68 CS.seek 0.000ml @10:22:24.72 Flushing lower manifold's waste2b port @10:22:24.73 CS Pulling 4ml of mfbkill @10:22:24.74 CSV.dial CSR @10:22:25.24 CSR.select :mfbkill @10:22:25.34 CS.reconfigure CSconfig @10:22:25.38 CS.seek 4.000ml @10:22:36.28 delay 5 seconds @10:22:41.29 CSR.select :air @10:22:41.38 CS.reconfigure CSfast @10:22:42.01 CS.seek 10.000ml @10:22:48.49 delay 5 seconds @10:22:53.50 ATV.dial ARV @10:22:54.02 ASV.dial :PCRlock @10:22:54.35 delay 5 seconds @10:22:56.39 CSV.dial CTV @10:22:56.41
CTV.dial CSV @10:22:56.92 CBV.dial :waste2b @10:22:57.67 CS.seek 0.000ml @10:22:59.36 Running HF183em ProfileEM60C2 thermocycling @10:22:59.38 PCR.program ProfileEM60C2 @10:22:59.48 ProfileEM60C2 should complete in < 2:11:38 @10:22:59.49 PCR.run 2:49:32.5 @10:23:20.91
Killing upper manifold @10:23:20.92 CS Pulling 4ml of mfbkill @10:23:20.93 CSV.dial CSR @10:23:21.44 CSR.select :mfbkill @10:23:21.54 CS.reconfigure CSconfig @10:23:21.58 CS.seek 4.000ml @10:23:32.56 delay 5 seconds @10:23:37.57 CSR.select :air @10:23:37.66 CS.reconfigure CSfast @10:23:37.70 CS.seek 10.000ml @10:23:52.00 CSV.dial CBV @10:23:52.03
CBV.dial CSV @10:23:53.05 CTV.dial :air @10:23:54.34 CS.seek 0.000ml @10:24:17.44 delay 7 seconds @10:24:24.45 CS.seek 3.000ml @10:24:32.01 CSV.dial :waste @10:24:32.44 CS.seek 0.000ml @10:24:40.01 Flushing upper manifold @10:24:40.10 CSV.dial :flush @10:24:40.75 CS.seek 5.000ml @10:24:52.79 delay 5 seconds @10:24:57.80 CSV.dial :air @10:24:58.37 CS.seek 7.000ml @10:25:03.69 CSV.dial CBV @10:25:03.71
CBV.dial CSV @10:25:04.78 CTV.dial :air @10:25:04.79 CS.seek 0.000ml @10:25:21.22 delay 7 seconds @10:25:28.23 CS.seek 3.000ml @10:25:35.82 CSV.dial :waste @10:25:36.33 CS.seek 0.000ml @10:25:44.03 CSV.dial :flush @10:25:44.45 CS.seek 3.000ml @10:25:52.10 delay 5 seconds @10:25:57.11 CSV.dial :air @10:25:57.60 CS.seek 5.000ml @10:26:02.94 CSV.dial :air @10:26:02.96 CS.seek 0.000ml @10:26:15.13 delay 7 seconds @10:26:22.14 CS.seek 3.000ml @10:26:29.81 CSV.dial :waste @10:26:30.62 CS.seek 0.000ml @10:26:38.22 Flushing lower manifold's waste1 port @10:26:38.31 CSV.dial :flush @10:26:38.82 CS.seek 5.000ml @10:26:50.88 delay 5 seconds @10:26:55.89 CSV.dial :air @10:26:56.38 CS.seek 7.000ml @10:27:01.81 CSV.dial CTV @10:27:01.83
CTV.dial CSV @10:27:03.16 CBV.dial :waste1 @10:27:03.62 CS.seek 0.000ml @10:27:20.22 Flushing lower manifold's waste2b port @10:27:20.31 CSV.dial :flush @10:27:21.10 CS.seek 5.000ml @10:27:33.12 delay 5 seconds @10:27:38.13 CSV.dial :air @10:27:38.67 CS.seek 7.000ml @10:27:44.00 CSV.dial CTV @10:27:44.02
CTV.dial CSV @10:27:44.68 CBV.dial :waste2b @10:27:45.41 CS.seek 0.000ml @10:28:01.87 Disinfecting external Intake with kill @10:28:01.96 CSV.dial CSR @10:28:02.48 CSR.select :kill @10:28:02.58 CS.seek 8.000ml @10:28:21.31 delay 5 seconds @10:28:26.32 CSR.select :air @10:28:26.41 CS.seek 10.000ml @10:28:31.78 CSV.dial CBV @10:28:31.80
CBV.dial CSV @10:28:32.83 CTV.dial :intake @10:28:33.54 Intake.select :open @10:28:33.65 CS.seek 2.000ml @10:28:52.59 Intake.select :close @10:28:52.68 CTV.dial :air @10:28:53.41 CS.reconfigure CSconfig @10:28:53.44 CS.seek 0.000ml @10:28:59.40 CSV.dial CSR @10:29:00.46 CSR.select :flush @10:29:00.56 CS.seek 5.000ml @10:29:14.06 delay 5 seconds @10:29:19.07 CSR.select :air @10:29:19.16 CS.seek 10.000ml @10:29:32.62 CSV.dial CBV @10:29:33.67 CS.seek 0.000ml @10:29:59.84 Garaging FlushPuck in Collection Clamp @10:29:59.91 Elbow.reconfigure TightElbow @10:29:59.94 Elbow.seek CC @10:30:00.04 Elbow.reconfigure LooseElbow @10:30:00.12 Forearm.seek CC @10:30:01.08 CC.seek sealed @10:30:02.02 Hand.grip @10:30:02.80 CC.seek open @10:30:06.11 Forearm.seek retract @10:30:07.07 Elbow.reconfigure TightElbow @10:30:07.10 Elbow.seek garage @10:30:09.92 Elbow.reconfigure LooseElbow @10:30:10.00 Forearm.reconfigure TightForearm @10:30:10.03 Forearm.seek garage @10:30:11.21 Hand.release @10:30:11.97 Forearm.reconfigure LooseForearm @10:30:12.00 Forearm.seek retract @10:30:13.04 Completed {wqPCR} begun 08:00:31 @10:30:13.07 Waiting for MFBPCR til 08:00PST16-Feb-14 @10:30:13.08 deferred until MFBPCR finish @10:30:13.09
Sleeping until 08:00PST16-Feb-14 @10:30:13.10 GoodNews.email "Then, in tube 2: wqPCR\n/home/esp/esp2local/mission/14feb14sccwrp.rb:63",Subject => "Waiting until 08:00PST16-Feb-14" @12:16:57.99 PCR.off @12:16:58.52 PCR for HF183em completed -- closed /var/log/esp/HF183em14feb1510h374ml.pcr @12:16:58.57 Cleaning PCR @12:16:58.58 AFV.select :carrierSolution @12:16:58.67 delay 2 seconds @12:17:00.68 AS.reconfigure ASconfig @12:17:00.71 AS.seek 0.500ml @12:17:11.14 delay 1 second @12:17:12.15 AFV.select :holdingCoil @12:17:12.24 ASV.dial :waste1 @12:17:12.72 ATV.dial :air3 @12:17:13.67 AS.reconfigure ASslow1 @12:17:13.70 AS.seek 0.510ml @12:17:15.92 delay 2 seconds @12:17:17.93 ATV.dial :bleach @12:17:18.31 AS.seek 0.810ml @12:18:19.75 delay 3 seconds @12:18:22.76 ATV.dial :air3 @12:18:23.11 delay 3 seconds @12:18:26.17 AS.seek 0.835ml @12:18:31.51 delay 3 seconds @12:18:34.52 ATV.dial :bleach @12:18:34.91 AS.seek 0.820ml @12:18:38.20 delay 3 seconds @12:18:41.21 ARV.dial :waste @12:18:41.81 delay 3 seconds @12:18:44.82 ATV.dial :ARV @12:18:45.58 AS.reconfigure ASconfig @12:18:45.61 AS.seek 0.470ml @12:19:06.29 AFV.select :carrierSolution @12:19:06.38 delay 2 seconds @12:19:08.39 AS.seek 1.000ml @12:19:39.57 delay 1 second @12:19:40.58 AFV.select :holdingCoil @12:19:40.67 delay 1 second @12:19:41.68 AS.reconfigure ASfast @12:19:41.71 AS.seek 0.000ml @12:20:09.45 AFV.select :carrierSolution @12:20:09.55 delay 2 seconds @12:20:11.56 AS.reconfigure ASconfig @12:20:11.59 AS.seek 1.000ml @12:21:10.21 delay 1 second @12:21:11.22 AFV.select :holdingCoil @12:21:11.31 delay 1 second @12:21:12.32 AS.reconfigure ASfast @12:21:12.35 AS.seek 0.000ml @12:21:40.08 AFV.select :carrierSolution @12:21:40.18 delay 2 seconds @12:21:42.19 AS.reconfigure ASconfig @12:21:42.22 AS.seek 1.000ml @12:22:40.82 delay 1 second @12:22:41.83 AFV.select :holdingCoil @12:22:41.92 delay 1 second @12:22:42.93 AS.reconfigure ASfast @12:22:42.96 AS.seek 0.000ml @12:23:10.71 ARV.dial between carrier and air1 @12:23:11.24 ATV.dial :air3 @12:23:12.21 AS.reconfigure ASslow1 @12:23:12.24 AS.seek 0.010ml @12:23:14.58 delay 2 seconds @12:23:16.59 ATV.dial :bleach @12:23:16.98 AS.seek 0.810ml @12:26:00.43 delay 3 seconds @12:26:03.44 ATV.dial :air3 @12:26:03.79 delay 3 seconds @12:26:06.85 AS.seek 0.835ml @12:26:12.20 delay 3 seconds @12:26:15.21 ATV.dial :bleach @12:26:15.60 AS.seek 0.820ml @12:26:18.91 delay 3 seconds @12:26:21.92 ATV.dial PCR @12:26:22.25 AS.reconfigure ASconfig @12:26:22.28 AS.seek empty @12:27:10.42 PCR.program :bleachProfile @12:27:10.47 PCR program should complete in < 2:45 @12:27:10.48 PCR.run 10:00 @12:30:11.69 PCR.off @12:30:11.72 AFV.select :carrierSolution @12:30:11.81 delay 2 seconds @12:30:13.82 AS.seek 1.000ml @12:31:12.48 delay 1 second @12:31:13.49 AFV.select :holdingCoil @12:31:13.58 delay 1 second @12:31:14.59 AS.reconfigure ASfast @12:31:14.62 AS.seek 0.000ml @12:31:42.36 delay 3 seconds @12:31:45.37 AFV.select :carrierSolution @12:31:45.46 delay 2 seconds @12:31:48.03 AS.reconfigure ASconfig @12:31:48.07 AS.seek 1.000ml @12:32:46.70 delay 1 second @12:32:47.71 AFV.select :holdingCoil @12:32:47.80 delay 1 second @12:32:48.81 AS.reconfigure ASfast @12:32:48.85 AS.seek 0.000ml @12:33:16.54 delay 3 seconds @12:33:19.55 AFV.select :carrierSolution @12:33:19.64 delay 2 seconds @12:33:21.65 AS.reconfigure ASconfig @12:33:21.68 AS.seek 1.000ml @12:34:20.29 delay 1 second @12:34:21.30 AFV.select :holdingCoil @12:34:21.39 delay 1 second @12:34:22.40 AS.reconfigure ASfast @12:34:22.43 AS.seek 0.000ml @12:34:50.15 delay 3 seconds @12:34:53.16 AFV.select :carrierSolution @12:34:53.25 delay 2 seconds @12:34:55.26 AS.reconfigure ASconfig @12:34:55.29 AS.seek 1.000ml @12:35:53.88 delay 1 second @12:35:54.89 AFV.select :holdingCoil @12:35:54.98 delay 1 second @12:35:55.99 AS.reconfigure ASfast @12:35:56.02 AS.seek 0.000ml @12:36:23.75 delay 3 seconds @12:36:26.76 AFV.select :carrierSolution @12:36:26.85 delay 2 seconds @12:36:28.86 AS.reconfigure ASconfig @12:36:28.89 AS.seek 1.000ml @12:37:27.52 delay 1 second @12:37:28.53 AFV.select :holdingCoil @12:37:28.62 delay 1 second @12:37:29.63 AS.reconfigure ASnorm @12:37:29.66 AS.seek 0.000ml @12:38:21.11 Setting up PCR for ENTap -- writing /var/log/esp/ENTap14feb1512h374ml.pcr @12:38:21.12 Building PCR Slug from ASV with MM1 mix, PP1 primer @12:38:21.13 Priming with carrier @12:38:21.14 ATV.dial ARV @12:38:21.66 ARV.dial :carrier @12:38:21.94 AS.seek 0.500ml @12:38:47.81 AFV.select :carrierSolution @12:38:47.92 delay 2 seconds @12:38:49.93 AS.reconfigure ASconfig @12:38:49.96 AS.seek 1.000ml @12:39:19.40 delay 1 second @12:39:20.41 AFV.select :holdingCoil @12:39:20.51 delay 1 second @12:39:21.52 ATV.dial :waste @12:39:22.79 AS.reconfigure ASnorm @12:39:22.82 AS.seek empty @12:40:14.29 AFV.select :carrierSolution @12:40:14.40 delay 2 seconds @12:40:16.41 AS.reconfigure ASconfig @12:40:16.44 AS.seek 0.500ml @12:40:45.94 delay 1 second @12:40:46.95 AFV.select :holdingCoil @12:40:47.05 Building Slug @12:40:47.06 ATV.dial :ARV @12:40:48.41 delay 2 seconds @12:40:50.42 ARV.dial :air1 @12:40:50.74 delay 2 seconds @12:40:52.80 AS.reconfigure ASslow2 @12:40:52.83 AS.seek 0.506ml @12:40:59.30 delay 2 seconds @12:41:01.31 ARV.dial :MM1 @12:41:01.80 delay 2 seconds @12:41:03.81 Adding MM1 @12:41:03.87 AS.seek 0.524ml @12:41:22.63 delay 25 seconds @12:41:47.64 ARV.dial :PP1 @12:41:47.98 Adding PP1 @12:41:47.99 delay 2 seconds @12:41:50.04 AS.seek 0.530ml @12:41:56.36 delay 10 seconds @12:42:06.37 ARV.dial :air1 @12:42:06.69 adding ARV air @12:42:06.70 delay 2 seconds @12:42:08.75 AS.seek 0.536ml @12:42:15.38 delay 5 seconds @12:42:20.39 ARV.dial :carrier @12:42:20.71 delay 7 seconds @12:42:27.77 AS.seek 0.594ml @12:43:27.36 delay 5 seconds @12:43:32.37 ATV.dial :ASV @12:43:32.72 delay 2 seconds @12:43:34.78 AS.seek 0.600ml @12:43:41.14 delay 4 seconds @12:43:45.15 ATV.dial ARV @12:43:45.48 delay 2 seconds @12:43:47.49 ARV.dial :carrier @12:43:47.50 delay 2 seconds @12:43:49.55 AS.seek 0.650ml @12:44:40.69 delay 4 seconds @12:44:44.70 ATV.dial PCR @12:44:45.29 ASV.dial :waste1 @12:44:45.30 ARV.dial :air1 @12:44:45.63 Positioning Slug by 0.324ml @12:44:45.68 AS.seek 0.326ml @12:50:16.33 delay 5 seconds @12:50:21.34 ATV.dial ARV @12:50:21.86 ASV.dial :PCRlock @12:50:22.35 delay 5 seconds @12:50:27.36 Running ENTap Profile60C thermocycling @12:50:27.37 PCR.program Profile60C @12:50:27.47 Profile60C should complete in < 1:39:08 @12:50:27.48 PCR.run 2:08:55 @14:12:12.19 PCR.off @14:12:12.71 PCR for ENTap completed -- closed /var/log/esp/ENTap14feb1512h374ml.pcr @14:12:12.76 Cleaning PCR @14:12:12.77 AFV.select :carrierSolution @14:12:12.86 delay 2 seconds @14:12:14.87 AS.reconfigure ASconfig @14:12:14.90 AS.seek 0.500ml @14:12:25.35 delay 1 second @14:12:26.36 AFV.select :holdingCoil @14:12:26.45 ASV.dial :waste1 @14:12:26.93 ATV.dial :air3 @14:12:27.88 AS.reconfigure ASslow1 @14:12:27.91 AS.seek 0.510ml @14:12:30.18 delay 2 seconds @14:12:32.19 ATV.dial :bleach @14:12:32.59 AS.seek 0.810ml @14:13:33.99 delay 3 seconds @14:13:37.00 ATV.dial :air3 @14:13:37.35 delay 3 seconds @14:13:40.41 AS.seek 0.835ml @14:13:45.75 delay 3 seconds @14:13:48.76 ATV.dial :bleach @14:13:49.15 AS.seek 0.820ml @14:13:52.46 delay 3 seconds @14:13:55.47 ARV.dial :waste @14:13:56.07 delay 3 seconds @14:13:59.08 ATV.dial :ARV @14:13:59.82 AS.reconfigure ASconfig @14:13:59.85 AS.seek 0.470ml @14:14:20.53 AFV.select :carrierSolution @14:14:20.62 delay 2 seconds @14:14:22.63 AS.seek 1.000ml @14:14:53.86 delay 1 second @14:14:54.87 AFV.select :holdingCoil @14:14:54.96 delay 1 second @14:14:55.97 AS.reconfigure ASfast @14:14:56.00 AS.seek 0.000ml @14:15:23.69 AFV.select :carrierSolution @14:15:23.79 delay 2 seconds @14:15:25.80 AS.reconfigure ASconfig @14:15:25.83 AS.seek 1.000ml @14:16:24.44 delay 1 second @14:16:25.45 AFV.select :holdingCoil @14:16:25.54 delay 1 second @14:16:26.55 AS.reconfigure ASfast @14:16:26.58 AS.seek 0.000ml @14:16:54.26 AFV.select :carrierSolution @14:16:54.36 delay 2 seconds @14:16:56.37 AS.reconfigure ASconfig @14:16:56.40 AS.seek 1.000ml @14:17:55.00 delay 1 second @14:17:56.01 AFV.select :holdingCoil @14:17:56.10 delay 1 second @14:17:57.11 AS.reconfigure ASfast @14:17:57.14 AS.seek 0.000ml @14:18:24.85 ARV.dial between carrier and air1 @14:18:25.26 ATV.dial :air3 @14:18:26.20 AS.reconfigure ASslow1 @14:18:26.23 AS.seek 0.010ml @14:18:28.59 delay 2 seconds @14:18:30.60 ATV.dial :bleach @14:18:31.00 AS.seek 0.810ml @14:21:14.41 delay 3 seconds @14:21:17.42 ATV.dial :air3 @14:21:17.75 delay 3 seconds @14:21:20.81 AS.seek 0.835ml @14:21:26.18 delay 3 seconds @14:21:29.19 ATV.dial :bleach @14:21:30.16 AS.seek 0.820ml @14:21:33.46 delay 3 seconds @14:21:36.47 ATV.dial PCR @14:21:36.80 AS.reconfigure ASconfig @14:21:36.83 AS.seek empty @14:22:24.94 PCR.program :bleachProfile @14:22:25.00 PCR program should complete in < 2:45 @14:22:25.01 PCR.run 10:00 @14:25:26.25 PCR.off @14:25:26.28 AFV.select :carrierSolution @14:25:26.37 delay 2 seconds @14:25:28.38 AS.seek 1.000ml @14:26:27.04 delay 1 second @14:26:28.05 AFV.select :holdingCoil @14:26:28.14 delay 1 second @14:26:29.15 AS.reconfigure ASfast @14:26:29.18 AS.seek 0.000ml @14:26:56.93 delay 3 seconds @14:26:59.94 AFV.select :carrierSolution @14:27:00.03 delay 2 seconds @14:27:02.04 AS.reconfigure ASconfig @14:27:02.07 AS.seek 1.000ml @14:28:00.69 delay 1 second @14:28:01.70 AFV.select :holdingCoil @14:28:01.79 delay 1 second @14:28:02.80 AS.reconfigure ASfast @14:28:02.83 AS.seek 0.000ml @14:28:30.56 delay 3 seconds @14:28:33.57 AFV.select :carrierSolution @14:28:33.66 delay 2 seconds @14:28:35.67 AS.reconfigure ASconfig @14:28:35.70 AS.seek 1.000ml @14:29:34.30 delay 1 second @14:29:35.31 AFV.select :holdingCoil @14:29:35.40 delay 1 second @14:29:36.41 AS.reconfigure ASfast @14:29:36.44 AS.seek 0.000ml @14:30:04.19 delay 3 seconds @14:30:07.20 AFV.select :carrierSolution @14:30:07.29 delay 2 seconds @14:30:09.30 AS.reconfigure ASconfig @14:30:09.33 AS.seek 1.000ml @14:31:07.93 delay 1 second @14:31:08.94 AFV.select :holdingCoil @14:31:09.03 delay 1 second @14:31:10.04 AS.reconfigure ASfast @14:31:10.22 AS.seek 0.000ml @14:31:37.98 delay 3 seconds @14:31:40.99 AFV.select :carrierSolution @14:31:41.08 delay 2 seconds @14:31:43.09 AS.reconfigure ASconfig @14:31:43.12 AS.seek 1.000ml @14:32:41.74 delay 1 second @14:32:42.75 AFV.select :holdingCoil @14:32:42.84 delay 1 second @14:32:43.85 AS.reconfigure ASnorm @14:32:43.88 AS.seek 0.000ml @14:33:35.41 Setting up PCR for ENTem -- writing /var/log/esp/ENTem14feb1514h374ml.pcr @14:33:35.42 Building PCR Slug from ASV with MM2 mix, PP3 primer @14:33:35.43 Priming with carrier ATV.dial ARV @14:33:35.94 ARV.dial :carrier @14:33:36.23 AS.seek 0.500ml @14:34:02.07 AFV.select :carrierSolution @14:34:02.16 delay 2 seconds @14:34:04.17 AS.reconfigure ASconfig @14:34:04.20 AS.seek 1.000ml @14:34:33.66 delay 1 second @14:34:34.67 AFV.select :holdingCoil @14:34:34.77 delay 1 second @14:34:35.78 ATV.dial :waste @14:34:37.14 AS.reconfigure ASnorm @14:34:37.17 AS.seek empty @14:35:28.64 AFV.select :carrierSolution @14:35:28.75 delay 2 seconds @14:35:30.76 AS.reconfigure ASconfig @14:35:30.79 AS.seek 0.500ml @14:36:00.27 delay 1 second @14:36:01.28 AFV.select :holdingCoil @14:36:01.38 Building Slug @14:36:01.39 ATV.dial :ARV @14:36:02.74 delay 2 seconds @14:36:04.75 ARV.dial :air1 @14:36:05.08 delay 2 seconds @14:36:07.14 AS.reconfigure ASslow2 @14:36:07.17 AS.seek 0.506ml @14:36:13.61 delay 2 seconds @14:36:15.62 ARV.dial :MM2 @14:36:16.94 delay 2 seconds @14:36:18.95 Adding MM2 @14:36:19.01 AS.seek 0.524ml @14:36:37.81 delay 25 seconds @14:37:02.82 ARV.dial :PP3 @14:37:03.14 Adding PP3 @14:37:03.15 delay 2 seconds @14:37:05.20 AS.seek 0.530ml @14:37:11.95 delay 10 seconds @14:37:21.96 ARV.dial :air2 @14:37:22.38 adding ARV air @14:37:22.39 delay 2 seconds @14:37:24.44 AS.seek 0.536ml @14:37:30.87 delay 5 seconds @14:37:35.88 ARV.dial :carrier @14:37:36.92 delay 7 seconds @14:37:43.98 AS.seek 0.594ml @14:38:43.45 delay 5 seconds @14:38:48.46 ATV.dial :ASV @14:38:48.79 delay 2 seconds @14:38:50.85 AS.seek 0.600ml @14:38:57.21 delay 4 seconds @14:39:01.22 ATV.dial ARV @14:39:01.54 delay 2 seconds @14:39:03.55 ARV.dial :carrier @14:39:03.56 delay 2 seconds @14:39:05.61 AS.seek 0.650ml @14:39:56.88 delay 4 seconds @14:40:00.89 ATV.dial PCR @14:40:01.50 ASV.dial :waste1 @14:40:01.51 ARV.dial :air1 @14:40:01.97 Positioning Slug by 0.324ml @14:40:02.02 AS.seek 0.326ml @14:45:33.29 delay 5 seconds @14:45:38.30 ATV.dial ARV @14:45:38.81 ASV.dial :PCRlock @14:45:39.31 delay 5 seconds @14:45:44.32 Running ENTem ProfileEM60C thermocycling @14:45:44.33 PCR.program ProfileEM60C @14:45:44.44 ProfileEM60C should complete in < 2:11:38 @14:45:44.45 PCR.run 2:49:32.5 @16:40:56.31 PCR.off @16:40:56.84 PCR for ENTem completed -- closed /var/log/esp/ENTem14feb1514h374ml.pcr @16:40:56.89 Cleaning PCR @16:40:56.90 AFV.select :carrierSolution @16:40:56.99 delay 2 seconds @16:40:59.00 AS.reconfigure ASconfig @16:40:59.03 AS.seek 0.500ml @16:41:09.46 delay 1 second @16:41:10.47 AFV.select :holdingCoil @16:41:10.56 ASV.dial :waste1 @16:41:11.31 ATV.dial :air3 @16:41:12.25 AS.reconfigure ASslow1 @16:41:12.28 AS.seek 0.510ml @16:41:14.62 delay 2 seconds @16:41:16.63 ATV.dial :bleach @16:41:17.11 AS.seek 0.810ml @16:42:18.54 delay 3 seconds @16:42:21.55 ATV.dial :air3 @16:42:21.87 delay 3 seconds @16:42:24.93 AS.seek 0.835ml @16:42:30.26 delay 3 seconds @16:42:33.27 ATV.dial :bleach @16:42:33.67 AS.seek 0.820ml @16:42:36.98 delay 3 seconds @16:42:39.99 ARV.dial :waste @16:42:40.49 delay 3 seconds @16:42:43.50 ATV.dial :ARV @16:42:44.79 AS.reconfigure ASconfig @16:42:44.83 AS.seek 0.470ml @16:43:05.49 AFV.select :carrierSolution @16:43:05.59 delay 2 seconds @16:43:07.60 AS.seek 1.000ml @16:43:38.79 delay 1 second @16:43:39.80 AFV.select :holdingCoil @16:43:39.89 delay 1 second @16:43:40.90 AS.reconfigure ASfast @16:43:40.94 AS.seek 0.000ml @16:44:08.66 AFV.select :carrierSolution @16:44:08.76 delay 2 seconds @16:44:10.77 AS.reconfigure ASconfig @16:44:10.80 AS.seek 1.000ml @16:45:09.39 delay 1 second @16:45:10.40 AFV.select :holdingCoil @16:45:10.49 delay 1 second @16:45:11.50 AS.reconfigure ASfast @16:45:11.53 AS.seek 0.000ml @16:45:39.27 AFV.select :carrierSolution @16:45:39.37 delay 2 seconds @16:45:41.38 AS.reconfigure ASconfig @16:45:41.41 AS.seek 1.000ml @16:46:40.02 delay 1 second @16:46:41.03 AFV.select :holdingCoil @16:46:41.12 delay 1 second @16:46:42.13 AS.reconfigure ASfast @16:46:42.16 AS.seek 0.000ml @16:47:09.87 ARV.dial between carrier and air1 @16:47:10.39 ATV.dial :air3 @16:47:11.33 AS.reconfigure ASslow1 @16:47:11.36 AS.seek 0.010ml @16:47:13.72 delay 2 seconds @16:47:15.73 ATV.dial :bleach @16:47:16.13 AS.seek 0.810ml @16:49:59.53 delay 3 seconds @16:50:02.54 ATV.dial :air3 @16:50:02.89 delay 3 seconds @16:50:05.95 AS.seek 0.835ml @16:50:11.28 delay 3 seconds @16:50:14.29 ATV.dial :bleach @16:50:14.68 AS.seek 0.820ml @16:50:17.99 delay 3 seconds @16:50:21.00 ATV.dial PCR @16:50:21.32 AS.reconfigure ASconfig @16:50:21.35 AS.seek empty @16:51:09.41 PCR.program :bleachProfile @16:51:09.47 PCR program should complete in < 2:45 @16:51:09.48 PCR.run 10:00 @16:54:10.69 PCR.off @16:54:10.72 AFV.select :carrierSolution @16:54:10.81 delay 2 seconds @16:54:12.82 AS.seek 1.000ml @16:55:11.41 delay 1 second @16:55:12.42 AFV.select :holdingCoil @16:55:12.51 delay 1 second @16:55:13.52 AS.reconfigure ASfast @16:55:13.55 AS.seek 0.000ml @16:55:41.28 delay 3 seconds @16:55:44.29 AFV.select :carrierSolution @16:55:44.38 delay 2 seconds @16:55:46.39 AS.reconfigure ASconfig @16:55:46.42 AS.seek 1.000ml @16:56:45.05 delay 1 second @16:56:46.06 AFV.select :holdingCoil @16:56:46.15 delay 1 second @16:56:47.16 AS.reconfigure ASfast @16:56:47.19 AS.seek 0.000ml @16:57:14.94 delay 3 seconds @16:57:17.95 AFV.select :carrierSolution @16:57:18.04 delay 2 seconds @16:57:20.05 AS.reconfigure ASconfig @16:57:20.08 AS.seek 1.000ml @16:58:18.71 delay 1 second @16:58:19.72 AFV.select :holdingCoil @16:58:19.81 delay 1 second @16:58:20.82 AS.reconfigure ASfast @16:58:20.85 AS.seek 0.000ml @16:58:48.58 delay 3 seconds @16:58:51.59 AFV.select :carrierSolution @16:58:51.68 delay 2 seconds @16:58:53.69 AS.reconfigure ASconfig @16:58:53.72 AS.seek 1.000ml @16:59:52.31 delay 1 second @16:59:53.32 AFV.select :holdingCoil @16:59:53.41 delay 1 second @16:59:54.42 AS.reconfigure ASfast @16:59:54.45 AS.seek 0.000ml @17:00:22.17 delay 3 seconds @17:00:25.18 AFV.select :carrierSolution @17:00:25.27 delay 2 seconds @17:00:27.28 AS.reconfigure ASconfig @17:00:27.31 AS.seek 1.000ml @17:01:25.94 delay 1 second @17:01:26.95 AFV.select :holdingCoil @17:01:27.04 delay 1 second @17:01:28.05 AS.reconfigure ASnorm @17:01:28.08 AS.seek 0.000ml @17:02:19.53 Setting up PCR for IPCap -- writing /var/log/esp/IPCap14feb1517h374ml.pcr @17:02:19.54 Building PCR Slug from ASV with MM1 mix, PP2 primer @17:02:19.55 Priming with carrier @17:02:19.56 ATV.dial ARV @17:02:20.07 ARV.dial :carrier @17:02:20.34 AS.seek 0.500ml @17:02:46.26 AFV.select :carrierSolution @17:02:46.37 delay 2 seconds @17:02:48.38 AS.reconfigure ASconfig @17:02:48.41 AS.seek 1.000ml @17:03:17.85 delay 1 second @17:03:18.86 AFV.select :holdingCoil @17:03:18.96 delay 1 second @17:03:19.97 ATV.dial :waste @17:03:21.32 AS.reconfigure ASnorm @17:03:21.35 AS.seek empty @17:04:12.82 AFV.select :carrierSolution @17:04:12.93 delay 2 seconds @17:04:14.94 AS.reconfigure ASconfig @17:04:14.97 AS.seek 0.500ml @17:04:44.46 delay 1 second @17:04:45.47 AFV.select :holdingCoil @17:04:45.57 Building Slug @17:04:45.58 ATV.dial :ARV @17:04:46.84 delay 2 seconds @17:04:48.85 ARV.dial :air1 @17:04:49.18 delay 2 seconds @17:04:51.24 AS.reconfigure ASslow2 @17:04:51.27 AS.seek 0.506ml @17:04:58.02 delay 2 seconds @17:05:00.03 ARV.dial :MM1 @17:05:00.62 delay 2 seconds @17:05:03.18 Adding MM1 @17:05:03.24 AS.seek 0.524ml @17:05:21.97 delay 25 seconds @17:05:46.98 ARV.dial :PP2 @17:05:47.42 Adding PP2 @17:05:47.43 delay 2 seconds @17:05:49.48 AS.seek 0.530ml @17:05:55.91 delay 10 seconds @17:06:05.92 ARV.dial :air2 @17:06:06.25 adding ARV air @17:06:06.26 delay 2 seconds @17:06:08.32 AS.seek 0.536ml @17:06:14.88 delay 5 seconds @17:06:19.89 ARV.dial :carrier @17:06:20.93 delay 7 seconds @17:06:27.99 AS.seek 0.594ml @17:07:27.45 delay 5 seconds @17:07:32.46 ATV.dial :ASV @17:07:32.78 delay 2 seconds @17:07:34.84 AS.seek 0.600ml @17:07:41.30 delay 4 seconds @17:07:45.31 ATV.dial ARV @17:07:45.64 delay 2 seconds @17:07:47.65 ARV.dial :carrier @17:07:47.66 delay 2 seconds @17:07:49.71 AS.seek 0.650ml @17:08:41.06 delay 4 seconds @17:08:45.07 ATV.dial PCR @17:08:45.56 ASV.dial :waste1 @17:08:45.57 ARV.dial :air1 @17:08:45.92 Positioning Slug by 0.324ml @17:08:45.97 AS.seek 0.326ml @17:14:16.62 delay 5 seconds @17:14:21.63 ATV.dial ARV @17:14:22.22 ASV.dial :PCRlock @17:14:22.56 delay 5 seconds @17:14:27.57 Running IPCap Profile59C thermocycling @17:14:27.58 PCR.program Profile59C @17:14:27.68 Profile59C should complete in < 1:39:08 @17:14:27.69 PCR.run 2:08:55 @18:37:29.21 PCR.off @18:37:29.75 PCR for IPCap completed -- closed /var/log/esp/IPCap14feb1517h374ml.pcr @18:37:29.80 Cleaning PCR @18:37:29.81 AFV.select :carrierSolution @18:37:29.90 delay 2 seconds @18:37:31.91 AS.reconfigure ASconfig @18:37:31.94 AS.seek 0.500ml @18:37:42.36 delay 1 second @18:37:43.37 AFV.select :holdingCoil @18:37:43.46 ASV.dial :waste1 @18:37:43.94 ATV.dial :air3 @18:37:44.88 AS.reconfigure ASslow1 @18:37:44.91 AS.seek 0.510ml @18:37:47.22 delay 2 seconds @18:37:49.23 ATV.dial :bleach @18:37:49.60 AS.seek 0.810ml @18:38:51.06 delay 3 seconds @18:38:54.07 ATV.dial :air3 @18:38:54.42 delay 3 seconds @18:38:57.48 AS.seek 0.835ml @18:39:02.81 delay 3 seconds @18:39:05.82 ATV.dial :bleach @18:39:06.22 AS.seek 0.820ml @18:39:09.54 delay 3 seconds @18:39:12.55 ARV.dial :waste @18:39:13.14 delay 3 seconds @18:39:16.15 ATV.dial :ARV @18:39:16.89 AS.reconfigure ASconfig @18:39:16.92 AS.seek 0.470ml @18:39:37.60 AFV.select :carrierSolution @18:39:37.70 delay 2 seconds @18:39:39.71 AS.seek 1.000ml @18:40:10.93 delay 1 second @18:40:11.94 AFV.select :holdingCoil @18:40:12.03 delay 1 second @18:40:13.04 AS.reconfigure ASfast @18:40:13.07 AS.seek 0.000ml @18:40:40.80 AFV.select :carrierSolution @18:40:40.90 delay 2 seconds @18:40:42.91 AS.reconfigure ASconfig @18:40:42.94 AS.seek 1.000ml @18:41:41.54 delay 1 second @18:41:42.55 AFV.select :holdingCoil @18:41:42.64 delay 1 second @18:41:43.65 AS.reconfigure ASfast @18:41:43.68 AS.seek 0.000ml @18:42:11.41 AFV.select :carrierSolution @18:42:11.51 delay 2 seconds @18:42:13.52 AS.reconfigure ASconfig @18:42:13.55 AS.seek 1.000ml @18:43:12.13 delay 1 second @18:43:13.14 AFV.select :holdingCoil @18:43:13.23 delay 1 second @18:43:14.24 AS.reconfigure ASfast @18:43:14.27 AS.seek 0.000ml @18:43:42.00 ARV.dial between carrier and air1 @18:43:42.52 ATV.dial :air3 @18:43:43.55 AS.reconfigure ASslow1 @18:43:43.58 AS.seek 0.010ml @18:43:45.96 delay 2 seconds @18:43:47.96 ATV.dial :bleach @18:43:48.44 AS.seek 0.810ml @18:46:31.82 delay 3 seconds @18:46:34.83 ATV.dial :air3 @18:46:35.15 delay 3 seconds @18:46:38.21 AS.seek 0.835ml @18:46:43.59 delay 3 seconds @18:46:46.60 ATV.dial :bleach @18:46:47.00 AS.seek 0.820ml @18:46:50.29 delay 3 seconds @18:46:53.30 ATV.dial PCR @18:46:53.62 AS.reconfigure ASconfig @18:46:53.65 AS.seek empty @18:47:41.76 PCR.program :bleachProfile @18:47:41.82 PCR program should complete in < 2:45 @18:47:41.83 PCR.run 10:00 @18:50:43.04 PCR.off @18:50:43.07 AFV.select :carrierSolution @18:50:43.16 delay 2 seconds @18:50:45.17 AS.seek 1.000ml @18:51:43.77 delay 1 second @18:51:44.78 AFV.select :holdingCoil @18:51:44.87 delay 1 second @18:51:45.88 AS.reconfigure ASfast @18:51:45.91 AS.seek 0.000ml @18:52:13.64 delay 3 seconds @18:52:16.65 AFV.select :carrierSolution @18:52:16.74 delay 2 seconds @18:52:18.75 AS.reconfigure ASconfig @18:52:18.78 AS.seek 1.000ml @18:53:17.37 delay 1 second @18:53:18.38 AFV.select :holdingCoil @18:53:18.47 delay 1 second @18:53:19.48 AS.reconfigure ASfast @18:53:19.51 AS.seek 0.000ml @18:53:47.25 delay 3 seconds @18:53:50.26 AFV.select :carrierSolution @18:53:50.35 delay 2 seconds @18:53:52.36 AS.reconfigure ASconfig @18:53:52.39 AS.seek 1.000ml @18:54:51.00 delay 1 second @18:54:52.01 AFV.select :holdingCoil @18:54:52.10 delay 1 second @18:54:53.11 AS.reconfigure ASfast @18:54:53.14 AS.seek 0.000ml @18:55:20.87 delay 3 seconds @18:55:23.88 AFV.select :carrierSolution @18:55:23.97 delay 2 seconds @18:55:25.98 AS.reconfigure ASconfig @18:55:26.01 AS.seek 1.000ml @18:56:24.58 delay 1 second @18:56:25.59 AFV.select :holdingCoil @18:56:25.68 delay 1 second @18:56:26.69 AS.reconfigure ASfast @18:56:26.72 AS.seek 0.000ml @18:56:54.46 delay 3 seconds @18:56:57.47 AFV.select :carrierSolution @18:56:57.56 delay 2 seconds @18:56:59.57 AS.reconfigure ASconfig @18:56:59.60 AS.seek 1.000ml @18:57:58.20 delay 1 second @18:57:59.21 AFV.select :holdingCoil @18:57:59.30 delay 1 second @18:58:00.84 AS.reconfigure ASnorm @18:58:00.88 AS.seek 0.000ml @18:58:52.33 Setting up PCR for IPCem -- writing /var/log/esp/IPCem14feb1518h374ml.pcr @18:58:52.34 Building PCR Slug from ASV with MM3 mix, PP5 primer @18:58:52.35 Priming with carrier @18:58:52.36 ATV.dial ARV @18:58:52.87 ARV.dial :carrier @18:58:53.14 AS.seek 0.500ml @18:59:19.01 AFV.select :carrierSolution @18:59:19.11 delay 2 seconds @18:59:21.12 AS.reconfigure ASconfig @18:59:21.16 AS.seek 1.000ml @18:59:50.61 delay 1 second @18:59:51.62 AFV.select :holdingCoil @18:59:51.71 delay 1 second @18:59:52.72 ATV.dial :waste @18:59:54.08 AS.reconfigure ASnorm @18:59:54.11 AS.seek empty @19:00:45.51 AFV.select :carrierSolution @19:00:45.62 delay 2 seconds @19:00:47.63 AS.reconfigure ASconfig @19:00:47.67 AS.seek 0.500ml @19:01:17.15 delay 1 second @19:01:18.16 AFV.select :holdingCoil @19:01:18.26 Building Slug @19:01:18.27 ATV.dial :ARV @19:01:19.60 delay 2 seconds @19:01:21.61 ARV.dial :air1 @19:01:21.93 delay 2 seconds @19:01:23.99 AS.reconfigure ASslow2 @19:01:24.02 AS.seek 0.506ml @19:01:30.40 delay 2 seconds @19:01:32.41 ARV.dial :MM3 @19:01:33.38 delay 2 seconds @19:01:35.39 Adding MM3 @19:01:35.45 AS.seek 0.524ml @19:01:54.26 delay 25 seconds @19:02:19.27 ARV.dial :PP5 @19:02:19.60 Adding PP5 @19:02:19.61 delay 2 seconds @19:02:21.67 AS.seek 0.530ml @19:02:28.18 delay 10 seconds @19:02:38.19 ARV.dial :air3 @19:02:38.62 adding ARV air @19:02:38.63 delay 2 seconds @19:02:40.69 AS.seek 0.536ml @19:02:47.07 delay 5 seconds @19:02:52.08 ARV.dial :carrier @19:02:53.10 delay 7 seconds @19:03:00.16 AS.seek 0.594ml @19:03:59.61 delay 5 seconds @19:04:04.62 ATV.dial :ASV @19:04:04.94 delay 2 seconds @19:04:07.00 AS.seek 0.600ml @19:04:13.47 delay 4 seconds @19:04:17.48 ATV.dial ARV @19:04:17.80 delay 2 seconds @19:04:19.81 ARV.dial :carrier @19:04:19.82 delay 2 seconds @19:04:21.87 AS.seek 0.650ml @19:05:12.99 delay 4 seconds @19:05:17.00 ATV.dial PCR @19:05:17.50 ASV.dial :waste1 @19:05:17.51 ARV.dial :air1 @19:05:17.86 Positioning Slug by 0.324ml @19:05:17.91 AS.seek 0.326ml @19:10:48.60 delay 5 seconds @19:10:53.61 ATV.dial ARV @19:10:54.13 ASV.dial :PCRlock @19:10:54.62 delay 5 seconds @19:10:59.63 Running IPCem ProfileEM59C thermocycling @19:10:59.64 PCR.program ProfileEM59C @19:10:59.80 ProfileEM59C should complete in < 2:11:38 @19:10:59.81 PCR.run 2:49:32.5 @21:06:28.39 PCR.off @21:06:28.93 PCR for IPCem completed -- closed /var/log/esp/IPCem14feb1518h374ml.pcr @21:06:28.94 Full MFB Decontamination @21:06:28.95 Decon extraction column and decon AIV @21:06:28.96 SPE.hold 2:46:40,55.0C @21:06:28.98 ASV.dial :SPEdelivery @21:06:29.94 delay 20 seconds @21:06:49.95 AFV.select :carrierSolution @21:06:50.04 delay 2 seconds @21:06:52.05 AS.reconfigure ASconfig @21:06:52.08 AS.seek 0.500ml @21:07:02.51 delay 1 second @21:07:03.52 AFV.select :holdingCoil @21:07:03.61 ATV.dial :air1 @21:07:05.20 AS.reconfigure ASslow1 @21:07:05.23 AS.seek 0.520ml @21:07:09.58 ATV.dial :ASV @21:07:10.88 ASV.dial :SPErecovery @21:07:11.59 AS.seek 0.620ml @21:07:32.23 ATV.dial :waste @21:07:33.68 AS.reconfigure ASnorm @21:07:33.71 AS.seek empty @21:08:05.70 AFV.select :holdingCoil @21:08:05.71 ATV.dial :waste @21:08:05.72 AS.reconfigure ASconfig @21:08:05.75 AS.seek 0.000ml @21:08:06.06 AFV.select :carrierSolution @21:08:06.17 delay 2 seconds @21:08:08.18 AS.seek 0.500ml @21:08:37.59 delay 1 second @21:08:38.60 AFV.select :holdingCoil @21:08:38.69 ATV.dial :air3 @21:08:39.26 AS.reconfigure ASslow1 @21:08:39.29 AS.seek 0.520ml @21:08:43.60 ATV.dial :air3 @21:08:43.66 AS.seek 0.530ml @21:08:45.96 delay 2 seconds @21:08:47.97 ATV.dial :bleach @21:08:48.34 AS.seek 0.710ml @21:09:25.21 delay 3 seconds @21:09:28.22 ATV.dial :air3 @21:09:28.56 delay 3 seconds @21:09:31.62 AS.seek 0.735ml @21:09:36.96 delay 3 seconds @21:09:39.97 ATV.dial :bleach @21:09:40.36 AS.seek 0.720ml @21:09:43.68 delay 3 seconds @21:09:46.69 ATV.dial ASV @21:09:47.57 ASV.dial :SPErecovery @21:09:47.63 AS.seek 0.365ml @21:11:00.29 ATV.dial :waste @21:11:01.75 AS.reconfigure ASnorm @21:11:01.78 AS.seek empty @21:11:20.80 ATV.dial :air3 @21:11:21.37 AS.reconfigure ASslow1 @21:11:21.40 AS.seek 0.009ml @21:11:23.72 delay 2 seconds @21:11:25.73 ATV.dial :bleach @21:11:26.10 AS.seek 0.579ml @21:13:22.60 delay 3 seconds @21:13:25.61 ATV.dial :air3 @21:13:25.95 delay 3 seconds @21:13:29.01 AS.seek 0.604ml @21:13:34.39 delay 3 seconds @21:13:37.40 ATV.dial :bleach @21:13:37.80 AS.seek 0.589ml @21:13:41.09 delay 3 seconds @21:13:44.10 ATV.dial :ASV @21:13:44.98 ASV.dial :wasteR @21:13:45.74 AS.seek 0.000ml @21:15:46.16 delay 1:00 @21:16:46.17 ATV.dial ASV @21:16:46.18 AFV.select :carrierSolution @21:16:46.27 delay 2 seconds @21:16:48.28 AS.reconfigure ASconfig @21:16:48.31 AS.seek 1.000ml @21:17:46.86 delay 1 second @21:17:48.45 AFV.select :holdingCoil @21:17:48.55 delay 1 second @21:17:49.56 ASV.dial :wasteR @21:17:49.57 AS.reconfigure ASslow1 @21:17:49.60 AS.seek 0.000ml @21:21:13.79 delay 10 seconds @21:21:23.80 ATV.dial :air3 @21:21:25.00 AS.seek 0.010ml @21:21:27.32 delay 2 seconds @21:21:29.33 ATV.dial :bleach @21:21:29.81 AS.seek 0.580ml @21:23:26.30 delay 3 seconds @21:23:29.31 ATV.dial :air3 @21:23:29.63 delay 3 seconds @21:23:32.69 AS.seek 0.605ml @21:23:37.96 delay 3 seconds @21:23:40.97 ATV.dial :bleach @21:23:41.37 AS.seek 0.590ml @21:23:44.69 delay 3 seconds @21:23:47.70 ATV.dial ASV @21:23:48.57 ASV.dial :SPEdelivery @21:23:49.05 AS.seek 0.000ml @21:25:49.64 delay 1:00 @21:26:49.65 AFV.select :carrierSolution @21:26:49.75 delay 2 seconds @21:26:51.76 AS.reconfigure ASconfig @21:26:51.79 AS.seek 1.000ml @21:27:50.37 delay 1 second @21:27:51.38 AFV.select :holdingCoil @21:27:51.48 delay 1 second @21:27:52.49 ATV.dial ASV @21:27:52.50 ASV.dial :SPEdelivery @21:27:52.51 AS.reconfigure ASslow1 @21:27:52.54 AS.seek 0.000ml @21:31:16.91 delay 10 seconds @21:31:26.92 ATV.dial :air2 @21:31:27.64 AS.reconfigure ASslow0 @21:31:27.67 AS.seek 0.800ml @21:32:49.47 delay 3 seconds @21:32:52.48 ATV.dial :ASV @21:32:53.30 ASV.dial :SPEdelivery @21:32:53.32 AS.reconfigure ASslow1 @21:32:53.35 AS.seek 0.030ml @21:35:30.67 delay 20 seconds @21:35:50.68 ATV.dial :waste,via => air3 @21:35:52.14 AS.reconfigure ASconfig @21:35:52.17 AS.seek empty @21:35:54.26 SPE.coast @21:35:54.34 Removing Bleach from Mixing Coil @21:35:54.35 AFV.select :carrierSolution @21:35:54.44 delay 2 seconds @21:35:56.45 AS.seek 0.500ml @21:36:25.93 delay 1 second @21:36:26.94 AFV.select :holdingCoil @21:36:27.03 ATV.dial :ASV @21:36:28.48 ASV.dial :SPErecovery @21:36:29.45 AS.reconfigure ASslow1 @21:36:29.48 AS.seek 0.730ml @21:37:16.63 ASV.dial :wasteD @21:37:17.30 AS.reconfigure ASnorm @21:37:17.33 AS.seek 0.000ml @21:37:54.94 AFV.select :carrierSolution @21:37:55.04 delay 2 seconds @21:37:57.05 AS.reconfigure ASconfig @21:37:57.08 AS.seek 0.500ml @21:38:26.52 delay 1 second @21:38:27.53 AFV.select :holdingCoil @21:38:27.62 ATV.dial ASV @21:38:27.63 ASV.dial :SPErecovery @21:38:28.34 AS.reconfigure ASslow0 @21:38:28.37 AS.seek 0.300ml @21:38:49.07 ATV.dial :air2 @21:38:49.93 AS.reconfigure ASslow1 @21:38:49.96 AS.seek 0.320ml @21:38:54.32 ATV.dial ASV @21:38:55.16 AS.reconfigure ASslow0 @21:38:55.19 AS.seek 0.570ml @21:39:20.93 ASV.dial :wasteD @21:39:21.61 AS.reconfigure ASnorm @21:39:21.64 AS.seek 0.000ml @21:39:51.08 AFV.select :carrierSolution @21:39:51.18 delay 2 seconds @21:39:53.19 AS.reconfigure ASconfig @21:39:53.22 AS.seek 0.500ml @21:40:22.67 delay 1 second @21:40:23.68 AFV.select :holdingCoil @21:40:23.77 ATV.dial ASV @21:40:23.78 ASV.dial :SPErecovery @21:40:24.49 AS.reconfigure ASslow0 @21:40:24.52 AS.seek 0.300ml @21:40:45.22 ATV.dial :air2 @21:40:46.07 AS.reconfigure ASslow1 @21:40:46.10 AS.seek 0.320ml @21:40:50.47 ATV.dial ASV @21:40:51.32 AS.reconfigure ASslow0 @21:40:51.35 AS.seek 0.570ml @21:41:17.16 ASV.dial :wasteD @21:41:18.09 AS.reconfigure ASnorm @21:41:18.12 AS.seek 0.000ml @21:41:47.58 ATV.dial :air2 @21:41:48.37 AS.reconfigure ASslow0 @21:41:48.40 AS.seek 0.300ml @21:42:19.34 ATV.dial :ASV @21:42:20.04 ASV.dial :wasteD @21:42:20.06 AS.reconfigure ASconfig @21:42:20.09 AS.seek 0.050ml @21:42:34.97 ATV.dial :waste @21:42:36.43 AS.seek 0.000ml @21:42:39.61 ASV.dial :SPEdelivery @21:42:40.45 deconLysateINT @21:42:40.46 ATV.dial :air3 @21:42:41.11 AS.reconfigure ASslow1 @21:42:41.14 AS.seek 0.010ml @21:42:43.48 delay 2 seconds @21:42:45.49 ATV.dial :bleach @21:42:45.86 AS.seek 0.960ml @21:45:59.87 delay 3 seconds @21:46:02.88 ATV.dial :air3 @21:46:03.21 delay 3 seconds @21:46:06.27 AS.seek 0.985ml @21:46:12.16 delay 3 seconds @21:46:15.17 ATV.dial :bleach @21:46:15.57 AS.seek 0.970ml @21:46:18.91 delay 3 seconds @21:46:21.92 ATV.dial :AIV @21:46:22.98 AIV.dial :coilToMFB @21:46:22.99 AWV.select :waste @21:46:23.00 AFV.select :carrierSolution @21:46:23.09 delay 2 seconds @21:46:25.10 AS.reconfigure ASconfig @21:46:25.14 AS.seek 1.000ml @21:46:27.25 delay 1 second @21:46:28.26 AFV.select :holdingCoil @21:46:28.36 delay 1 second @21:46:29.37 AS.reconfigure ASslow0 @21:46:29.40 AS.seek empty @21:48:11.79 AFV.select :carrierSolution @21:48:11.90 delay 2 seconds @21:48:13.91 AS.reconfigure ASconfig @21:48:13.94 AS.seek 1.000ml @21:49:12.57 delay 1 second @21:49:13.58 AFV.select :holdingCoil @21:49:13.67 delay 1 second @21:49:14.68 AS.reconfigure ASslow0 @21:49:14.71 AS.seek empty @21:50:57.10 AFV.select :carrierSolution @21:50:57.20 delay 2 seconds @21:50:59.21 AS.reconfigure ASconfig @21:50:59.24 AS.seek 1.000ml @21:51:57.88 delay 1 second @21:51:58.89 AFV.select :holdingCoil @21:51:58.98 delay 1 second @21:51:59.99 AS.reconfigure ASslow0 @21:52:00.02 AS.seek empty @21:53:42.42 ATV.dial :air2 @21:53:43.00 AS.reconfigure ASslow1 @21:53:43.03 AS.seek 0.009ml @21:53:45.39 AS.reconfigure ASslow0 @21:53:45.43 AS.seek 1.000ml @21:55:26.79 ATV.dial AIV @21:55:27.34 AS.seek 0.020ml @21:57:07.64 ATV.dial :waste @21:57:09.02 AS.seek 0.000ml @21:57:11.39 AWV.select :air @21:57:11.54 Cleaning PCR @21:57:11.55 AFV.select :carrierSolution @21:57:11.64 delay 2 seconds @21:57:13.65 AS.reconfigure ASconfig @21:57:13.68 AS.seek 0.500ml @21:57:43.12 delay 1 second @21:57:44.13 AFV.select :holdingCoil @21:57:44.22 ASV.dial :waste1 @21:57:44.56 ATV.dial :air3 @21:57:45.14 AS.reconfigure ASslow1 @21:57:45.17 AS.seek 0.510ml @21:57:47.46 delay 2 seconds @21:57:49.47 ATV.dial :bleach @21:57:49.84 AS.seek 0.810ml @21:58:51.26 delay 3 seconds @21:58:54.27 ATV.dial :air3 @21:58:54.62 delay 3 seconds @21:58:57.68 AS.seek 0.835ml @21:59:03.05 delay 3 seconds @21:59:06.06 ATV.dial :bleach @21:59:06.45 AS.seek 0.820ml @21:59:09.77 delay 3 seconds @21:59:12.78 ARV.dial :waste @21:59:13.27 delay 3 seconds @21:59:16.28 ATV.dial :ARV @21:59:17.03 AS.reconfigure ASconfig @21:59:17.06 AS.seek 0.470ml @21:59:37.74 AFV.select :carrierSolution @21:59:37.84 delay 2 seconds @21:59:39.85 AS.seek 1.000ml @22:00:11.04 delay 1 second @22:00:12.05 AFV.select :holdingCoil @22:00:12.14 delay 1 second @22:00:13.15 AS.reconfigure ASfast @22:00:13.18 AS.seek 0.000ml @22:00:40.91 AFV.select :carrierSolution @22:00:41.01 delay 2 seconds @22:00:43.02 AS.reconfigure ASconfig @22:00:43.05 AS.seek 1.000ml @22:01:41.64 delay 1 second @22:01:42.65 AFV.select :holdingCoil @22:01:42.74 delay 1 second @22:01:43.75 AS.reconfigure ASfast @22:01:43.78 AS.seek 0.000ml @22:02:11.52 AFV.select :carrierSolution @22:02:11.62 delay 2 seconds @22:02:13.63 AS.reconfigure ASconfig @22:02:13.66 AS.seek 1.000ml @22:03:12.26 delay 1 second @22:03:13.27 AFV.select :holdingCoil @22:03:13.36 delay 1 second @22:03:14.37 AS.reconfigure ASfast @22:03:14.40 AS.seek 0.000ml @22:03:42.12 ARV.dial between carrier and air1 @22:03:42.52 ATV.dial :air3 @22:03:43.46 AS.reconfigure ASslow1 @22:03:43.49 AS.seek 0.010ml @22:03:45.85 delay 2 seconds @22:03:47.86 ATV.dial :bleach @22:03:48.34 AS.seek 0.810ml @22:06:31.80 delay 3 seconds @22:06:34.81 ATV.dial :air3 @22:06:35.14 delay 3 seconds @22:06:38.20 AS.seek 0.835ml @22:06:43.55 delay 3 seconds @22:06:46.56 ATV.dial :bleach @22:06:46.96 AS.seek 0.820ml @22:06:50.28 delay 3 seconds @22:06:53.29 ATV.dial PCR @22:06:53.61 AS.reconfigure ASconfig @22:06:53.64 AS.seek empty @22:07:41.77 PCR.program :bleachProfile @22:07:41.83 PCR program should complete in < 2:45 @22:07:41.84 PCR.run 10:00 @22:10:46.09 PCR.off @22:10:46.12 AFV.select :carrierSolution @22:10:46.22 delay 2 seconds @22:10:48.23 AS.seek 1.000ml @22:11:46.88 delay 1 second @22:11:47.89 AFV.select :holdingCoil @22:11:47.98 delay 1 second @22:11:48.99 AS.reconfigure ASfast @22:11:49.02 AS.seek 0.000ml @22:12:16.77 delay 3 seconds @22:12:19.78 AFV.select :carrierSolution @22:12:19.88 delay 2 seconds @22:12:21.89 AS.reconfigure ASconfig @22:12:21.92 AS.seek 1.000ml @22:13:20.52 delay 1 second @22:13:21.53 AFV.select :holdingCoil @22:13:21.62 delay 1 second @22:13:22.63 AS.reconfigure ASfast @22:13:22.66 AS.seek 0.000ml @22:13:50.41 delay 3 seconds @22:13:53.42 AFV.select :carrierSolution @22:13:53.51 delay 2 seconds @22:13:55.52 AS.reconfigure ASconfig @22:13:55.55 AS.seek 1.000ml @22:14:54.14 delay 1 second @22:14:55.15 AFV.select :holdingCoil @22:14:55.24 delay 1 second @22:14:56.25 AS.reconfigure ASfast @22:14:56.28 AS.seek 0.000ml @22:15:24.02 delay 3 seconds @22:15:27.03 AFV.select :carrierSolution @22:15:27.12 delay 2 seconds @22:15:29.13 AS.reconfigure ASconfig @22:15:29.16 AS.seek 1.000ml @22:16:27.78 delay 1 second @22:16:28.79 AFV.select :holdingCoil @22:16:28.88 delay 1 second @22:16:29.89 AS.reconfigure ASfast @22:16:29.92 AS.seek 0.000ml @22:16:57.65 delay 3 seconds @22:17:00.66 AFV.select :carrierSolution @22:17:00.75 delay 2 seconds @22:17:02.76 AS.reconfigure ASconfig @22:17:02.79 AS.seek 1.000ml @22:18:01.40 delay 1 second @22:18:02.41 AFV.select :holdingCoil @22:18:02.50 delay 1 second @22:18:03.51 AS.reconfigure ASnorm @22:18:03.54 AS.seek 0.000ml @22:18:55.00 AFV.select :carrierSolution @22:18:55.09 delay 2 seconds @22:18:57.10 AS.reconfigure ASconfig @22:18:57.13 AS.seek 1.000ml @22:19:55.73 delay 1 second @22:19:56.74 AFV.select :holdingCoil @22:19:56.83 delay 1 second @22:19:57.84 ATV.dial :waste @22:19:58.74 AS.reconfigure ASnorm @22:19:58.77 AS.seek 0.500ml @22:20:24.71 ATV.dial :waste2 @22:20:25.92 AS.seek 0.000ml @22:20:51.79 AS.stop @22:20:51.81 CC.stop @22:20:51.84 CS.stop @22:20:51.86 PC.stop @22:20:51.89 PS.stop @22:20:51.92 Forearm.stop @22:20:51.95 Elbow.stop @22:20:51.98 SS.stop @22:20:52.05 Sleepy.power :raw,:OFF @22:20:52.06 Sleepy.power :dwarf,:OFF Shutdown @22:20:52.07 GoodNews.email "MFBPCR completed",Subject => "Shutdown" @08:00:31.73PST16-Feb-14
Powering up Dwarves @08:00:36.95 Sleepy.power :raw,:ON @08:00:37.42 SPE.configure SPEconfig @08:00:37.44 AS.configure ASconfig @08:00:37.47 CC.configure CCconfig @08:00:37.50 CS.configure CSconfig @08:00:37.53 PC.configure PCconfig @08:00:37.56 PS.configure PSconfig @08:00:37.59 Forearm.configure LooseForearm @08:00:37.62 Elbow.configure LooseElbow @08:00:37.65 SC.configure SCloose @08:00:37.68 SE.configure SEconfig @08:00:37.71 SS.configure SSconfig @08:00:37.74 CH.configure CHconfig @08:00:37.76 PH.configure PHconfig @08:00:37.79 Valve.reset @08:00:38.45 PCR.off @08:00:38.47 PCR.pidtemp= 50,0.1,0,300,25,25 @08:00:38.50 PCR.caltemp= 6.6661,0.0344,0.0 @08:00:39.07 Intake.select :close @08:00:39.11 Exhaust.select :close @08:00:39.12 CTV.dial :air @08:00:39.16 PSV.dial :air @08:00:39.19
PC.reconfigure PCconfig @08:00:39.24 PC.home.seek open @08:00:39.26 CSV.dial :waste1 @08:00:39.72 SSV.dial :spit,avoiding => exhaust @08:00:39.78 PS.reconfigure PSconfig @08:00:39.85 PS.home.seek 0.000ml @08:00:40.56 CS.reconfigure CSfast @08:00:40.59 SS.reconfigure SSconfig @08:00:40.69 SS.home.seek 0.00ml @08:00:40.70 CS.home.seek 0.000ml @08:00:40.78 CC.reconfigure CCconfig @08:00:40.82 CC.home.seek open @08:00:50.09 PS.coast @08:00:50.17
CC.seek open @08:00:50.19 PC.seek open @08:00:50.35
Forearm.reconfigure LooseForearm @08:00:50.38 Forearm.home.seek retract @08:00:51.12 Hand.grip @08:00:51.14
Elbow.jog -1500 @08:00:51.66 Elbow.reconfigure LooseElbow @08:00:51.69 Elbow.home.seek clear @08:00:56.43 SE.reconfigure SEconfig @08:00:56.46 SE.home.seek down @08:00:57.00 SC.reconfigure SCsloppy @08:00:57.04 SC.home.seek initial @08:00:59.23 Forearm.seek retract @08:00:59.29 Elbow.seek hammer @08:01:00.45 Forearm.reconfigure TightForearm @08:01:00.50 Forearm.seek 2 @08:01:01.55 Forearm.seek retract @08:01:02.63 Forearm.reconfigure LooseForearm @08:01:03.05PST16-Feb-14 ===> STARTED wqPCR (phase #3) <=== @08:01:03.06 Starting initialPurge @08:01:03.07 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:01:03.12 Hand.release @08:01:03.95 Elbow.reconfigure TightElbow @08:01:03.98 Elbow.seek FlushPuck::Garage @08:01:05.75 Elbow.reconfigure LooseElbow @08:01:05.83 Forearm.seek garage @08:01:06.84 Hand.grip @08:01:07.70 Forearm.seek retract @08:01:08.73 Elbow.reconfigure TightElbow @08:01:08.77 Elbow.seek CC @08:01:11.54 Elbow.reconfigure LooseElbow @08:01:11.62 Forearm.reconfigure TightForearm @08:01:11.65 Forearm.seek CC @08:01:12.73 CC.seek closed @08:01:16.49 Collection Clamp onSpring/offSpring for 5/22 samples, on/off torque 1.91 @08:01:16.55 Hand.release @08:01:17.28 Forearm.reconfigure LooseForearm @08:01:17.31 Forearm.seek retract @08:01:18.24 Flushing the Collection Valve::Manifold @08:01:18.33 CS Pulling 5ml of flush @08:01:18.34 CSV.dial CSR @08:01:19.61 CSR.select :flush @08:01:19.71 CS.reconfigure CSconfig @08:01:19.75 CS.seek 5.000ml @08:01:33.15 delay 5 seconds @08:01:38.17 CSR.select :air @08:01:38.31 CS.reconfigure CSfast @08:01:38.34 CS.seek 7.500ml @08:01:44.90 CSV.dial :waste @08:01:46.15 CS.reconfigure CSconfig @08:01:46.19 CS.seek 0.000ml @08:02:05.96 Purging external Intake @08:02:06.05 CSV.dial :flush @08:02:06.71 CS.reconfigure CSfast @08:02:06.74 CS.seek 10.000ml @08:02:30.40 delay 5 seconds @08:02:35.41 CSV.dial CBV @08:02:35.43
CBV.dial CSV @08:02:36.14 CTV.dial :intake @08:02:36.89 Intake.select :open @08:02:36.99 CS.seek 0.000ml @08:03:01.21 Intake.select :close @08:03:01.30 CTV.dial :air @08:03:02.04 Flushing upper manifold @08:03:02.13 CSV.dial :flush @08:03:02.93 CS.seek 5.000ml @08:03:15.24 delay 5 seconds @08:03:20.25 CSV.dial :air @08:03:20.73 CS.seek 7.000ml @08:03:26.12 CSV.dial CBV @08:03:26.14
CBV.dial CSV @08:03:27.21 CTV.dial :air @08:03:27.23 CS.seek 0.000ml @08:03:44.05 delay 7 seconds @08:03:51.07 CS.seek 3.000ml @08:03:58.80 CSV.dial :waste @08:03:59.31 CS.seek 0.000ml @08:04:07.32 CSV.dial :flush @08:04:07.75 CS.seek 3.000ml @08:04:15.52 delay 5 seconds @08:04:20.53 CSV.dial :air @08:04:20.99 CS.seek 5.001ml @08:04:26.43 CSV.dial :air @08:04:26.44 CS.seek 0.000ml @08:04:38.86 delay 7 seconds @08:04:45.88 CS.seek 3.000ml @08:04:53.55 CSV.dial :waste @08:04:54.34 CS.seek 0.000ml @08:05:02.14 Garaging FlushPuck in Collection Clamp @08:05:02.20 Elbow.reconfigure TightElbow @08:05:02.23 Elbow.seek CC @08:05:02.45 Elbow.reconfigure LooseElbow @08:05:02.54 Forearm.seek CC @08:05:03.48 CC.seek sealed @08:05:04.43 Hand.grip @08:05:05.10 CC.seek open @08:05:08.39 Forearm.seek retract @08:05:09.33 Elbow.reconfigure TightElbow @08:05:09.37 Elbow.seek garage @08:05:12.71 Elbow.reconfigure LooseElbow @08:05:12.80 Forearm.reconfigure TightForearm @08:05:12.83 Forearm.seek garage @08:05:14.00 Hand.release @08:05:14.77 Forearm.reconfigure LooseForearm @08:05:14.80 Forearm.seek retract @08:05:15.91 Starting mfb @08:05:15.92 pcrBAC sampling at most 1000ml, wcr at most 1000ml @08:05:15.94 Loading sh1 puck from tube 2 into Collection Clamp @08:05:15.97 BACfans.email "Can@08:01:03, 14.6C, 61% humidity, 14.1psia, 13.734V, 0.616A, 0.610A avg, 8.46W\nCTD data unavailable\nISUS data unavailable",Subject => "pcrBAC sampling at most 1000ml, wcr at most 1000ml" @08:05:17.95 Readying the MFB @08:05:17.98 Homing MFB Syringe AFV.select :holdingCoil @08:05:18.19
SC.reconfigure SCloose @08:05:18.21 ATV.dial :air2 @08:05:18.41
Elbow.reconfigure TightElbow @08:05:18.45 Elbow.seek 2 @08:05:18.59 AS.reconfigure ASconfig @08:05:18.62 AS.home.seek home @08:05:20.27
Forearm.reconfigure TightForearm @08:05:20.30 Forearm.seek 2 + 800 counts @08:05:21.32 SE.reconfigure SEpick @08:05:21.37 SE.seek beamBreak @08:05:23.19 SE.configure SEconfig @08:05:23.33 Elbow.seek 2 @08:05:23.47 Forearm.seek 2 + 300 counts @08:05:23.69 Forearm.reconfigure LooseForearm @08:05:23.73 Hand.grip @08:05:24.50 SE.seek down @08:05:24.62
Forearm.seek retract @08:05:25.60 Elbow.seek CC @08:05:26.04 SE.coast @08:05:26.13 AFV.select :carrierSolution @08:05:26.24 delay 2 seconds @08:05:27.17
Elbow.reconfigure LooseElbow @08:05:27.25 Forearm.reconfigure TightForearm @08:05:27.28 Forearm.seek CC @08:05:28.31 CC.seek closed @08:05:28.33 AS.seek 1.000ml @08:05:32.14
Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 2.00 @08:05:32.15 Hand.release @08:05:32.89 Forearm.reconfigure LooseForearm @08:05:32.92 Forearm.seek retract @08:05:33.87 SC.coast @08:05:33.90 collectSample 1000ml w/bubble point of 55psi @08:05:33.91 Priming 7ml @08:05:33.92 Sleepy.power :sampler,:ON Intake.select :close @08:05:33.93 Exhaust.select :close @08:05:33.94 SS.hold @08:05:34.09 Calibrating pressure and temperature sensors @08:05:34.11 CTV.dial :air @08:05:34.12 SSV.dial :spit,avoiding => exhaust @08:05:34.13 SS.reconfigure SSfast @08:05:34.16 SS.seek 5.00ml @08:05:37.27 SSV.dial CBV @08:05:37.29
CBV.dial SSV @08:05:38.64 delay 3.8 seconds @08:05:42.52 SSV.dial :spit @08:05:43.85 SS.seek 0.00ml @08:05:46.50 SSV.dial CBV @08:05:47.83 CTV.dial :intake @08:05:48.58 Waiting for external sampler to finish priming... @08:05:48.59 delayUntil "08:15:33.91" @08:06:20.06 delay 1 second @08:06:21.07 AFV.select :holdingCoil @08:06:21.16 delay 1 second @08:06:22.17 ATV.dial :waste @08:06:23.56 AS.reconfigure ASnorm @08:06:23.59 AS.home.seek empty @08:07:16.73 ATV.dial :waste @08:07:16.74 ARV.dial between carrier and air1 @08:07:16.80 ASV.dial :waste1 @08:15:33.91 SSV.dial CBV @08:15:33.93
CBV.dial SSV @08:15:33.94 Allowing 55s for 7.0ml of remaining 7.0ml @08:15:33.95 Intake.select :open @08:15:36.08 Intake @26.5psia, vacuum from 20.0 to 24.0psi @08:15:36.09 SS.reconfigure forSampling @08:15:36.13 SS.seek 7.00ml @08:15:41.66 Stroke completed -- waiting for pressure to equalize ... @08:15:42.89 Intake.select :close @08:15:43.03 SSV.dial :exhaust @08:15:43.80 Exhaust.select :open @08:15:43.89 SS.reconfigure SSexhale @08:15:43.93 SS.seek 0.00ml @08:15:47.86 Exhaust.select :close @08:15:47.96 Sampling 1000.0ml @08:15:47.97 Intake.select :close @08:15:47.98 Exhaust.select :close @08:15:47.99 SS.reconfigure SSconfig @08:15:48.02 SS.hold @08:15:48.17 CTV.dial :intake @08:15:48.18 SSV.dial CBV @08:15:48.20
CBV.dial SSV @08:15:48.98 Allowing 185s for 25.0ml of remaining 1000.0ml Intake.select :open @08:15:51.11 Intake @25.9psia, vacuum from 19.4 to 23.4psi @08:15:51.12 SS.reconfigure forSampling @08:15:51.16 SS.seek 25.00ml @08:15:55.53 Vacuum too high -- waiting up to 40s @08:15:55.80 Intake @17.3psia, vacuum from 10.8 to 14.8psi @08:15:55.81 SS.reconfigure forSampling @08:15:55.84 SS.seek 25.00ml @08:15:59.09 Vacuum too high -- waiting up to 40s @08:15:59.78 Intake @17.4psia, vacuum from 10.9 to 14.9psi @08:15:59.79 SS.reconfigure forSampling @08:15:59.82 SS.seek 25.00ml @08:16:09.25 Stroke completed -- waiting for pressure to equalize ... @08:16:09.89 Intake.select :close @08:16:10.02 SSV.dial :exhaust @08:16:10.78 Exhaust.select :open @08:16:10.87 SS.reconfigure SSexhale @08:16:10.91 SS.seek 0.00ml @08:16:19.70 Exhaust.select :close @08:16:19.79 SSV.dial CBV @08:16:19.81
CBV.dial SSV @08:16:20.58 Allowing 185s for 25.0ml of remaining 975.0ml @08:16:20.59 Intake.select :open @08:16:22.72 Intake @23.7psia, vacuum from 17.2 to 21.2psi @08:16:22.73 SS.reconfigure forSampling @08:16:22.77 SS.seek 25.00ml @08:16:28.28 Vacuum too high -- waiting up to 40s @08:16:28.54 Intake @15.6psia, vacuum from 9.1 to 13.1psi @08:16:28.55 SS.reconfigure forSampling @08:16:28.59 SS.seek 25.00ml @08:16:41.53 Stroke completed -- waiting for pressure to equalize ... @08:16:42.17 Intake.select :close @08:16:42.30 SSV.dial :exhaust @08:16:43.06 Exhaust.select :open @08:16:43.15 SS.reconfigure SSexhale @08:16:43.19 SS.seek 0.00ml @08:16:51.72 Exhaust.select :close @08:16:51.81 SSV.dial CBV @08:16:51.83
CBV.dial SSV @08:16:52.59 Allowing 185s for 25.0ml of remaining 950.0ml @08:16:52.60 Intake.select :open @08:16:54.73 Intake @22.0psia, vacuum from 15.5 to 19.5psi @08:16:54.74 SS.reconfigure forSampling @08:16:54.78 SS.seek 25.00ml @08:17:00.35 Vacuum too high -- waiting up to 40s @08:17:00.62 Intake @15.4psia, vacuum from 8.9 to 12.9psi @08:17:00.63 SS.reconfigure forSampling @08:17:00.66 SS.seek 25.00ml @08:17:11.44 Vacuum too high -- waiting up to 40s @08:17:12.13 Intake @15.8psia, vacuum from 9.3 to 13.3psi @08:17:12.14 SS.reconfigure forSampling @08:17:12.18 SS.seek 25.00ml @08:17:17.54 Stroke completed -- waiting for pressure to equalize ... @08:17:18.18 Intake.select :close @08:17:18.31 SSV.dial :exhaust @08:17:19.07 Exhaust.select :open @08:17:19.16 SS.reconfigure SSexhale @08:17:19.20 SS.seek 0.00ml @08:17:27.82 Exhaust.select :close @08:17:27.91 SSV.dial CBV @08:17:27.93
CBV.dial SSV @08:17:28.68 Allowing 185s for 25.0ml of remaining 925.0ml @08:17:28.69 Intake.select :open @08:17:30.82 Intake @20.4psia, vacuum from 13.9 to 17.9psi @08:17:30.83 SS.reconfigure forSampling @08:17:30.87 SS.seek 25.00ml @08:17:45.34 Vacuum too high -- waiting up to 40s @08:17:45.61 Intake @15.4psia, vacuum from 8.9 to 12.9psi @08:17:45.62 SS.reconfigure forSampling @08:17:45.65 SS.seek 25.00ml @08:17:58.60 Stroke completed -- waiting for pressure to equalize ... @08:17:59.23 Intake.select :close @08:17:59.37 SSV.dial :exhaust @08:18:00.13 Exhaust.select :open @08:18:00.22 SS.reconfigure SSexhale @08:18:00.26 SS.seek 0.00ml @08:18:09.17 Exhaust.select :close @08:18:09.78 CBV.dial SSV @08:18:09.79 SSV.dial CBV @08:18:10.57
Allowing 185s for 25.0ml of remaining 900.0ml @08:18:10.58 Intake.select :open @08:18:12.72 Intake @19.0psia, vacuum from 12.5 to 16.5psi @08:18:12.73 SS.reconfigure forSampling @08:18:12.77 SS.seek 25.00ml @08:18:29.09 Vacuum too high -- waiting up to 40s @08:18:29.58 Intake @15.5psia, vacuum from 9.0 to 13.0psi @08:18:29.59 SS.reconfigure forSampling @08:18:29.63 SS.seek 25.00ml @08:18:48.39 Stroke completed -- waiting for pressure to equalize ... @08:18:49.03 Intake.select :close @08:18:49.18 SSV.dial :exhaust @08:18:49.94 Exhaust.select :open @08:18:50.05 SS.reconfigure SSexhale @08:18:50.09 SS.seek 0.00ml @08:18:59.17 Exhaust.select :close @08:18:59.26 SSV.dial CBV @08:18:59.28
CBV.dial SSV @08:19:00.05 Allowing 185s for 25.0ml of remaining 875.0ml @08:19:00.06 Intake.select :open @08:19:02.19 Intake @18.1psia, vacuum from 11.6 to 15.6psi @08:19:02.20 SS.reconfigure forSampling @08:19:02.24 SS.seek 25.00ml @08:19:28.56 Vacuum too high -- waiting up to 40s @08:19:29.25 Intake @15.5psia, vacuum from 9.0 to 13.0psi @08:19:29.26 SS.reconfigure forSampling @08:19:29.30 SS.seek 25.00ml @08:19:49.90 Stroke completed -- waiting for pressure to equalize ... @08:19:51.13 Intake.select :close @08:19:51.28 SSV.dial :exhaust @08:19:52.03 Exhaust.select :open @08:19:52.13 SS.reconfigure SSexhale @08:19:52.17 SS.seek 0.00ml @08:20:00.90 Exhaust.select :close @08:20:00.99 SSV.dial CBV @08:20:01.01
CBV.dial SSV @08:20:01.78 Allowing 185s for 25.0ml of remaining 850.0ml @08:20:01.79 Intake.select :open @08:20:03.92 Intake @17.1psia, vacuum from 10.6 to 14.6psi @08:20:03.93 SS.reconfigure forSampling @08:20:03.97 SS.seek 25.00ml @08:20:43.90 Vacuum too high -- waiting up to 40s @08:20:45.24 Intake @15.6psia, vacuum from 9.1 to 13.1psi @08:20:45.25 SS.reconfigure forSampling @08:20:45.28 SS.seek 25.00ml @08:21:22.56 Stroke completed -- waiting for pressure to equalize ... @08:21:23.78 Intake.select :close @08:21:23.91 SSV.dial :exhaust @08:21:24.67 Exhaust.select :open @08:21:24.76 SS.reconfigure SSexhale @08:21:24.80 SS.seek 0.00ml @08:21:33.63 Exhaust.select :close @08:21:33.72 SSV.dial CBV @08:21:33.74
CBV.dial SSV @08:21:34.51 Allowing 185s for 25.0ml of remaining 825.0ml @08:21:34.52 Intake.select :open @08:21:36.65 Intake @16.4psia, vacuum from 9.9 to 13.9psi @08:21:36.66 SS.reconfigure forSampling @08:21:36.70 SS.seek 25.00ml @08:23:27.41 Stroke completed -- waiting for pressure to equalize ... @08:23:32.70 Intake.select :close @08:23:32.84 SSV.dial :exhaust @08:23:33.61 Exhaust.select :open @08:23:33.70 SS.reconfigure SSexhale @08:23:33.74 SS.seek 0.00ml @08:23:42.53 Exhaust.select :close @08:23:42.62 SSV.dial CBV @08:23:42.64
CBV.dial SSV @08:23:43.42 Allowing 185s for 25.0ml of remaining 800.0ml @08:23:43.43 Intake.select :open @08:23:45.56 Intake @15.8psia, vacuum from 9.3 to 13.3psi @08:23:45.57 SS.reconfigure forSampling @08:23:45.61 SS.seek 25.00ml @08:23:57.05 Vacuum too high -- waiting up to 40s @08:23:59.00 Intake @15.4psia, vacuum from 8.9 to 12.9psi @08:23:59.01 SS.seek 25.00ml @08:24:09.45 Vacuum too high -- waiting up to 40s @08:24:12.25 Intake @15.3psia, vacuum from 8.8 to 12.8psi @08:24:12.27 SS.seek 25.00ml @08:24:24.40 Vacuum too high -- waiting up to 40s @08:24:28.32 Intake @15.4psia, vacuum from 8.9 to 12.9psi @08:24:28.33 SS.seek 25.00ml @08:24:41.95 Vacuum too high -- waiting up to 40s @08:24:47.22 Intake @15.2psia, vacuum from 8.7 to 12.7psi @08:24:47.23 SS.seek 25.00ml @08:25:03.70 Vacuum too high -- waiting up to 40s @08:25:11.25 Intake @15.3psia, vacuum from 8.8 to 12.8psi @08:25:11.26 SS.seek 25.00ml @08:25:32.45 Vacuum too high -- waiting up to 40s @08:25:42.93 Intake @15.2psia, vacuum from 8.7 to 12.7psi @08:25:42.94 SS.seek 25.00ml @08:26:06.07 Vacuum too high -- waiting up to 40s @08:26:20.11 Intake @15.1psia, vacuum from 8.6 to 12.6psi @08:26:20.12 SS.seek 25.00ml @08:26:23.59 Stroke completed -- waiting for pressure to equalize ... @08:26:35.35 Intake.select :close @08:26:35.48 SSV.dial :exhaust @08:26:36.24 Exhaust.select :open @08:26:36.34 SS.reconfigure SSexhale @08:26:36.38 SS.seek 0.00ml @08:26:45.41 Exhaust.select :close @08:26:45.50 SSV.dial CBV @08:26:45.52
CBV.dial SSV @08:26:46.31 Allowing 185s for 25.0ml of remaining 775.0ml @08:26:46.32 Intake.select :open @08:26:48.45 Intake @15.4psia, vacuum from 8.9 to 12.9psi @08:26:48.46 SS.reconfigure forSampling @08:26:48.50 SS.seek 25.00ml @08:26:53.60 Vacuum too high -- waiting up to 40s @08:26:56.02 Intake @15.1psia, vacuum from 8.6 to 12.6psi @08:26:56.03 SS.seek 25.00ml @08:27:00.47 Vacuum too high -- waiting up to 40s @08:27:03.30 Intake @15.2psia, vacuum from 8.7 to 12.7psi @08:27:03.32 SS.seek 25.00ml @08:27:07.50 Vacuum too high -- waiting up to 40s @08:27:10.32 Intake @15.1psia, vacuum from 8.6 to 12.6psi @08:27:10.33 SS.seek 25.00ml @08:27:15.19 Vacuum too high -- waiting up to 40s @08:27:19.08 Intake @15.2psia, vacuum from 8.7 to 12.7psi @08:27:19.10 SS.seek 25.00ml @08:27:25.19 Vacuum too high -- waiting up to 40s @08:27:29.01 Intake @15.2psia, vacuum from 8.7 to 12.7psi @08:27:29.02 SS.seek 25.00ml @08:27:33.85 Vacuum too high -- waiting up to 40s @08:27:37.36 Intake @15.2psia, vacuum from 8.7 to 12.7psi @08:27:37.37 SS.seek 25.00ml @08:27:42.56 Vacuum too high -- waiting up to 40s @08:27:46.66 Intake @15.2psia, vacuum from 8.7 to 12.7psi @08:27:46.68 SS.seek 25.00ml @08:27:53.31 Vacuum too high -- waiting up to 40s @08:27:58.50 Intake @15.1psia, vacuum from 8.6 to 12.6psi @08:27:58.51 SS.seek 25.00ml @08:28:05.69 Vacuum too high -- waiting up to 40s @08:28:11.65 SS.seek 25.00ml @08:28:18.84 Vacuum too high -- waiting up to 40s @08:28:25.21 Intake @15.1psia, vacuum from 8.6 to 12.6psi @08:28:25.22 SS.seek 25.00ml @08:28:32.25 Vacuum too high -- waiting up to 40s @08:28:38.50 Intake @15.2psia, vacuum from 8.7 to 12.7psi @08:28:38.51 SS.seek 25.00ml @08:28:45.91 Vacuum too high -- waiting up to 40s @08:28:52.90 Intake @15.1psia, vacuum from 8.6 to 12.6psi @08:28:52.92 SS.seek 25.00ml @08:29:00.31 Vacuum too high -- waiting up to 40s @08:29:07.98 Intake @15.2psia, vacuum from 8.7 to 12.7psi @08:29:07.99 SS.seek 25.00ml @08:29:15.22 Vacuum too high -- waiting up to 40s @08:29:22.33 Intake @15.1psia, vacuum from 8.6 to 12.6psi @08:29:22.34 SS.seek 25.00ml @08:29:30.81 Vacuum too high -- waiting up to 40s @08:29:39.71 Intake @15.1psia, vacuum from 8.6 to 12.6psi @08:29:39.72 SS.seek 25.00ml @08:29:48.60 Vacuum too high -- waiting up to 40s @08:29:51.32 SS.reconfigure SSconfig @08:29:51.36 SS.hold @08:29:51.45 Intake.select :close @08:29:51.59 ShallowSampler::Clogged in MAIN -- progress too slow after 247.6ml SSV.dial :exhaust @08:29:52.36 Exhaust.select :open @08:29:52.47 SS.reconfigure SSexhale @08:29:52.51 SS.seek 0.00ml @08:30:00.94 Exhaust.select :close @08:30:01.04 Sampled 247.6ml @08:30:01.05 collectSample: Complete Evacuating Puck (bubblePt=55.0psi, delay=15.0s) @08:30:01.06 Intake.select :close @08:30:01.12 CSV.dial :waste @08:30:01.15 CS.seek 0.000ml @08:30:01.24 SSV.dial CBV,avoiding => exhaust @08:30:01.26
CBV.dial SSV @08:30:01.31 CSV.dial :air @08:30:02.11 CS.seek 10.000ml @08:30:03.16
CTV.dial :air @08:30:03.93 delay 15 seconds @08:30:04.03 Sleepy.power :sampler,:OFF @08:30:18.93
SSV.dial :spit,avoiding => exhaust @08:30:20.26 CTV.dial CSV,avoiding => intake @08:30:20.28
SS.seek 0.00ml @08:30:20.38 SSV.dial CBV,avoiding => exhaust @08:30:26.23 CBV.dial between CSV and waste2b @08:30:27.17 CTV.dial CSV,avoiding => air @08:30:27.19
CSV.dial CTV @08:30:27.64 CS.reconfigure CSpressurize @08:30:27.67 CS.seek 0.000ml @08:30:53.52 CSV.dial :air @08:30:54.05 CS.reconfigure CSfast @08:30:54.08 CS.seek 10.000ml @08:31:17.53 CSV.dial between air and CTV @08:31:17.92 CS.seek 2.817ml @08:31:35.13 CTV.dial CSV,avoiding => air @08:31:35.14
CSV.dial CTV @08:31:35.44 CS.reconfigure CSpressurize @08:31:35.47 CS.seek 0.000ml @08:31:40.98 BubblePoint: Intake @69.9psia, Outlet @14.5psia SS.reconfigure SSconfig @08:31:41.02 SS.seek 10.00ml @08:31:46.76 delay 15 seconds @08:32:01.86 Evacuated: Intake @69.2psia, Outlet @-0.2psia SSV.dial :spit,avoiding => exhaust @08:32:03.19 SS.reconfigure SSexhale @08:32:03.22 SS.seek 0.00ml @08:32:08.05 SS.reconfigure SSfast @08:32:08.08 SS.seek 5.00ml @08:32:10.92 SSV.dial CBV,avoiding => exhaust @08:32:10.94
CBV.dial SSV @08:32:12.27 CTV.dial :air,avoiding => intake @08:32:13.58 CSV.dial CTV @08:32:13.60
SS.reconfigure SSconfig @08:32:13.64 SS.seek 10.00ml @08:32:14.60 CSV.dial :air @08:32:15.70 CS.reconfigure CSfast @08:32:15.74 CS.seek 0.000ml @08:32:16.87
SSV.dial :spit,avoiding => exhaust @08:32:18.19 SS.reconfigure SSexhale @08:32:18.22 SS.seek 0.00ml @08:32:18.46 CS.coast @08:32:23.66
SS.reconfigure SSfast @08:32:23.70 SS.seek 5.00ml @08:32:26.69 SSV.dial CBV,avoiding => exhaust @08:32:26.71
CBV.dial SSV @08:32:29.01 SSV.dial :spit,avoiding => exhaust @08:32:30.31 SS.reconfigure SSconfig @08:32:30.34 SS.seek 0.00ml @08:32:33.41 SS.coast @08:32:33.44 Evacuating Puck (bubblePt=55.0psi, delay=15.0s) Intake.select :close @08:32:33.50 CSV.dial :waste @08:32:33.60 SSV.dial CBV,avoiding => exhaust @08:32:33.62
CBV.dial SSV @08:32:34.25 CS.seek 0.000ml @08:32:34.30 CSV.dial :air @08:32:34.92
CTV.dial :air @08:32:34.93 delay 15 seconds @08:32:35.09 CS.seek 10.000ml @08:32:49.93
SSV.dial :spit,avoiding => exhaust @08:32:51.23 CTV.dial CSV,avoiding => intake @08:32:51.25
SS.reconfigure SSexhale @08:32:51.33 SS.seek 0.00ml @08:32:51.71 SSV.dial CBV,avoiding => exhaust @08:32:58.85 CBV.dial between CSV and waste2b @08:32:59.79 CSV.dial between air and CTV @08:33:00.09 CS.seek 8.417ml @08:33:04.59 CTV.dial CSV,avoiding => air @08:33:04.60
CSV.dial CTV @08:33:04.89 CS.reconfigure CSpressurize @08:33:04.92 CS.seek 0.000ml @08:33:26.80 CSV.dial :air @08:33:27.31 CS.reconfigure CSfast @08:33:27.34 CS.seek 10.000ml @08:33:50.90 CSV.dial between air and CTV @08:33:51.29 CS.seek 3.032ml @08:34:07.97 CTV.dial CSV,avoiding => air @08:34:07.98
CSV.dial CTV @08:34:08.49 CS.reconfigure CSpressurize @08:34:08.52 CS.seek 0.000ml @08:34:15.89 BubblePoint: Intake @71.6psia, Outlet @16.3psia @08:34:15.90 SS.reconfigure SSconfig @08:34:15.93 SS.seek 10.00ml @08:34:21.75 delay 15 seconds @08:34:36.85 Evacuated: Intake @70.3psia, Outlet @0.2psia SSV.dial :spit,avoiding => exhaust @08:34:38.18 SS.reconfigure SSexhale @08:34:38.21 SS.seek 0.00ml @08:34:43.15 SS.reconfigure SSfast @08:34:43.18 SS.seek 5.00ml @08:34:46.27 SSV.dial CBV,avoiding => exhaust @08:34:46.29
CBV.dial SSV @08:34:47.63 CTV.dial :air,avoiding => intake @08:34:48.93 CSV.dial CTV @08:34:48.95
SS.reconfigure SSconfig @08:34:48.99 SS.seek 10.00ml @08:34:49.95 CSV.dial :air @08:34:50.38 CS.reconfigure CSfast @08:34:50.41 CS.seek 0.000ml @08:34:52.02 CS.coast @08:34:52.13
SSV.dial :spit,avoiding => exhaust @08:34:53.44 SS.reconfigure SSexhale @08:34:53.47 SS.seek 0.00ml @08:34:58.65 SS.reconfigure SSfast @08:34:58.68 SS.seek 5.00ml @08:35:01.80 SSV.dial CBV,avoiding => exhaust @08:35:01.82
CBV.dial SSV @08:35:04.12 SSV.dial :spit,avoiding => exhaust @08:35:05.42 SS.reconfigure SSconfig @08:35:05.45 SS.seek 0.00ml @08:35:08.52 SS.coast @08:35:08.55 Lysing: Adding 1.5ml lysis CBV.dial between CSV and waste1,avoiding => DBV @08:35:10.03 CTV.dial CSV @08:35:11.31 CSV.dial CSR @08:35:12.09 CSR.select :lysis @08:35:12.19 CS.reconfigure CSconfig @08:35:12.23 CS.seek 1.000ml @08:35:15.52 delay 5 seconds @08:35:20.53 CSR.select :air @08:35:20.67 CS.seek 6.000ml @08:35:34.15 delay 2 seconds @08:35:36.16 CSV.dial :waste1 @08:35:37.42 CS.reconfigure CSfast @08:35:37.45 CS.seek empty @08:35:51.83 CSV.dial CSR @08:35:53.12 CSR.select :lysis @08:35:53.22 CS.reconfigure CSconfig @08:35:53.26 CS.seek 1.500ml @08:35:57.93 delay 5 seconds @08:36:03.43 CSR.select :air @08:36:03.57 CS.seek 4.500ml @08:36:12.02 delay 2 seconds @08:36:14.03 CSV.dial CTV @08:36:14.55 CS.seek 1.275ml @08:36:23.62 Heating sh1 puck from tube 2 in Collection Clamp to 85.0C for 5:00 @08:36:23.63 CH.seek 85.0C,8:00 @08:39:23.76 CH.hold 5:00,85.0C @08:44:23.85 Collection Puck Heater at 85.0C after lysing @08:44:23.86 CS.seek 0.000ml @08:44:28.60 CTV.dial between spare and air,avoiding => intake @08:44:29.59 CSV.dial :air @08:44:30.04 CS.seek 5.000ml @08:44:43.50 CSV.dial CTV @08:44:44.01 delay 2 seconds @08:44:46.02 CSV.dial :air @08:44:46.53 delay 2 seconds @08:44:48.54 CSV.dial CTV @08:44:49.26 delay 2 seconds @08:44:51.27 CSV.dial :air @08:44:51.70 delay 2 seconds @08:44:53.71 CS.seek 0.000ml @08:45:07.19 Recovering Lysate into Collection syringe @08:45:07.20 CSV.dial CBV @08:45:08.19 CBV.dial CSV @08:45:08.55 CS.seek 5.000ml @08:45:22.03 delay 5 seconds @08:45:27.04 CTV.dial :air @08:45:27.53 CS.seek 7.000ml @08:45:33.43 delay 5 seconds @08:45:38.44 CSV.dial :air2 @08:45:38.87 CS.seek 10.000ml @08:45:47.25 CSV.dial between air and CTV,avoiding => CBV @08:45:48.40 Lysing Complete @08:45:48.45 Storing sh1 puck in Collection Clamp to tube 1 @08:45:48.58 SC.reconfigure SCtight @08:45:48.61 SC.seek 1 @08:45:50.84 SC.reconfigure SCloose @08:45:51.02 Elbow.reconfigure TightElbow @08:45:51.05 Elbow.seek 1 @08:45:52.67 Forearm.reconfigure TightForearm @08:45:52.70 Forearm.seek 1 + 800 counts @08:45:53.79 SE.reconfigure SEpick @08:45:53.84 SE.seek beamBreak @08:45:56.02 SE.configure SEconfig @08:45:56.14 Elbow.reconfigure LimpElbow @08:45:56.17 Forearm.reconfigure LooseForearm @08:45:56.21 Forearm.seek 1 @08:45:56.43 Elbow.hold @08:45:56.48 Elbow.reconfigure TightElbow @08:45:56.56 SE.seek 8.04 Pucks below Top Plate @08:45:56.90 Forearm.coast @08:45:56.93 Forearm.seek 1 + 328 counts @08:45:57.17 Forearm.reconfigure TightForearm @08:45:57.20 Forearm.seek 1 + 800 counts @08:45:57.43 SE.seek 9.04 Pucks below Top Plate @08:45:57.98 Forearm.reconfigure LooseForearm @08:45:58.01 Forearm.seek retract @08:45:58.93 Elbow.seek CC @08:46:00.48 Elbow.reconfigure LooseElbow @08:46:00.56 Forearm.seek CC @08:46:01.50 CC.seek sealed @08:46:02.43 Hand.grip @08:46:03.14 CC.seek open @08:46:06.48 Forearm.seek retract @08:46:07.42 Elbow.reconfigure TightElbow @08:46:07.46 Elbow.seek 1 @08:46:09.05 Elbow.reconfigure LimpElbow @08:46:09.09 Forearm.seek 1 @08:46:10.02 Elbow.hold @08:46:10.07 Elbow.reconfigure TightElbow @08:46:10.11 Hand.release @08:46:10.97 Forearm.coast @08:46:11.00 Forearm.seek 1 + 347 counts @08:46:11.23 Forearm.reconfigure TightForearm @08:46:11.26 Forearm.seek 1 + 800 counts @08:46:11.57 SE.seek down @08:46:12.68
Forearm.reconfigure LooseForearm @08:46:12.71 Forearm.seek retract @08:46:13.41 SE.coast @08:46:13.62
SC.coast @08:46:13.65 Tranferring lysate to PRV @08:46:13.66 CSV.dial :air @08:46:13.94 CS.seek 10.000ml @08:46:14.00 CSV.dial between air and CTV @08:46:14.93 AIV.dial :bypass @08:46:15.01 Beginning samplePassC @08:46:15.02 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:46:15.16 Elbow.seek FlushPuck::Garage @08:46:16.93 Elbow.reconfigure LooseElbow @08:46:17.01 Forearm.seek garage @08:46:17.99 Hand.grip @08:46:18.86 Forearm.seek retract @08:46:19.87 Elbow.reconfigure TightElbow @08:46:19.91 Elbow.seek CC @08:46:22.68 Elbow.reconfigure LooseElbow @08:46:22.76 Forearm.reconfigure TightForearm @08:46:22.79 Forearm.seek CC @08:46:23.92 CC.seek closed @08:46:27.65 Collection Clamp onSpring/offSpring for 5/22 samples, on/off torque 2.08 @08:46:27.70 Hand.release @08:46:28.43 Forearm.reconfigure LooseForearm @08:46:28.46 Forearm.seek retract @08:46:29.43 AWV.select :air @08:46:29.44 AIV.dial :bypass @08:46:29.45 PSV.dial PSR @08:46:30.73 PSR.select :flush @08:46:30.83 PS.seek 1.500ml @08:46:46.88 delay 5 seconds @08:46:51.90 PSV.dial :waste1 @08:46:52.35 PS.seek 0.000ml @08:47:08.63 PSV.dial PSR @08:47:09.09 PSR.select :air @08:47:09.19 PS.seek 2.500ml @08:47:35.39 CBV.dial PRV @08:47:35.40 PRV.dial CBV @08:47:36.16
PSV.dial :mixloop @08:47:37.16 CTV.dial :air,avoiding => intake @08:47:37.17 PTV.dial :PRVmixing @08:47:37.25 PS.seek 0.000ml @08:48:03.44 PSV.dial :air @08:48:03.89 PS.seek 2.500ml @08:48:30.10 PSV.dial :mixloop @08:48:30.55 PS.seek 0.000ml @08:48:56.79 PSV.dial :air @08:48:57.23 PS.seek 2.500ml @08:49:23.41 PSV.dial :mixloop @08:49:23.86 PS.seek 0.000ml @08:49:50.07 Garaging FlushPuck in Collection Clamp @08:49:50.13 Elbow.reconfigure TightElbow @08:49:50.16 Elbow.seek CC @08:49:50.25 Elbow.reconfigure LooseElbow @08:49:50.33 Forearm.seek CC @08:49:51.24 CC.seek sealed @08:49:52.17 Hand.grip @08:49:52.88 CC.seek open @08:49:56.17 Forearm.seek retract @08:49:57.16 Elbow.reconfigure TightElbow @08:49:57.20 Elbow.seek garage @08:49:59.91 Elbow.reconfigure LooseElbow @08:50:00.00 Forearm.reconfigure TightForearm @08:50:00.03 Forearm.seek garage @08:50:01.20 Hand.release @08:50:01.92 Forearm.reconfigure LooseForearm @08:50:01.95 Forearm.seek retract @08:50:02.97 Connecting PS to CS to transfer sample @08:50:02.98 CBV.dial AIV @08:50:02.99 AIV.dial :bypass @08:50:03.00 PSV.dial :mixloop @08:50:03.01 PTV.dial :PRVmixing PRV.dial AIV @08:50:03.02 CTV.dial :air,avoiding => intake @08:50:03.03 Loading lyfil puck from tube 2 into Collection Clamp @08:50:03.21 SC.reconfigure SCtight @08:50:03.24 SC.seek 2 @08:50:05.43 SC.reconfigure SCloose @08:50:05.59 Elbow.reconfigure TightElbow @08:50:05.63 Elbow.seek 2 @08:50:08.03 Forearm.reconfigure TightForearm @08:50:08.07 Forearm.seek 2 + 800 counts @08:50:09.21 SE.reconfigure SEpick @08:50:09.26 SE.seek beamBreak @08:50:11.15 SE.configure SEconfig @08:50:11.27 Elbow.seek 2 @08:50:11.43 Forearm.seek 2 + 300 counts @08:50:11.64 Forearm.reconfigure LooseForearm @08:50:11.68 Hand.grip @08:50:12.39 SE.seek down @08:50:12.53
Forearm.seek retract @08:50:13.49 Elbow.seek CC @08:50:14.08 SE.coast @08:50:15.05
Elbow.reconfigure LooseElbow @08:50:15.13 Forearm.reconfigure TightForearm @08:50:15.16 Forearm.seek CC @08:50:16.24 CC.seek closed @08:50:19.99 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 2.03 @08:50:20.00 Hand.release @08:50:20.80 Forearm.reconfigure LooseForearm @08:50:20.83 Forearm.seek retract @08:50:21.81 SC.coast @08:50:21.84 CTV.dial CSV,avoiding => intake @08:50:23.14 CSV.dial CTV @08:50:23.43 CS.seek 5.000ml @08:50:36.95 delay 1:20 @08:51:56.97 CS.seek 2.500ml @08:52:04.08 delay 40 seconds @08:52:44.09 CS.seek 0.000ml @08:52:51.24 delay 1:00 @08:53:51.25 PSV.dial :air @08:53:51.70 PS.seek 2.500ml @08:54:17.97 PSV.dial :mixloop @08:54:18.41 delay 3 seconds @08:54:21.42 PSV.dial :air @08:54:21.86 delay 2 seconds @08:54:23.87 PSV.dial :mixloop @08:54:24.30 delay 3 seconds @08:54:27.31 PSV.dial :waste1 @08:54:28.61 PS.seek empty @08:54:54.85 CBV.dial :waste1 @08:54:55.31 delay 5 seconds @08:55:00.32 CBV.dial AIV @08:55:00.78 delay 10 seconds @08:55:10.79 CBV.dial :waste1 @08:55:11.26 delay 10 seconds @08:55:21.27 CBV.dial AIV @08:55:21.73 delay 10 seconds @08:55:31.74 CTV.dial CSV,avoiding => intake @08:55:31.75 CBV.dial between AIV and waste1 @08:55:32.07 CSV.dial :air @08:55:32.58 CS.seek 10.000ml @08:55:58.70 CSV.dial CTV @08:55:59.21 CS.seek 0.000ml @08:56:25.29 CBV.dial :waste1 @08:56:25.60 delay 10 seconds @08:56:35.61 CTV.dial :air,avoiding => intake @08:56:36.97 Storing lyfil puck in Collection Clamp to tube 1 @08:56:37.10 SC.reconfigure SCtight @08:56:37.13 SC.seek 1 @08:56:39.30 SC.reconfigure SCloose @08:56:39.47 Elbow.reconfigure TightElbow @08:56:39.51 Elbow.seek 1 @08:56:41.11 Forearm.reconfigure TightForearm @08:56:41.14 Forearm.seek 1 + 800 counts @08:56:42.25 SE.reconfigure SEpick @08:56:42.30 SE.seek beamBreak @08:56:44.35 SE.configure SEconfig @08:56:44.47 Elbow.reconfigure LimpElbow @08:56:44.50 Forearm.reconfigure LooseForearm @08:56:44.54 Forearm.seek 1 @08:56:44.77 Elbow.hold @08:56:44.82 Elbow.reconfigure TightElbow @08:56:44.90 SE.seek 9.05 Pucks below Top Plate @08:56:45.24 Forearm.coast @08:56:45.27 Forearm.seek 1 + 343 counts @08:56:45.51 Forearm.reconfigure TightForearm @08:56:45.54 Forearm.seek 1 + 800 counts @08:56:45.81 SE.seek 10.05 Pucks below Top Plate @08:56:46.43 Forearm.reconfigure LooseForearm @08:56:46.46 Forearm.seek retract @08:56:47.90 Elbow.seek CC @08:56:49.50 Elbow.reconfigure LooseElbow @08:56:49.58 Forearm.seek CC @08:56:50.55 CC.seek sealed @08:56:51.48 Hand.grip @08:56:52.25 CC.seek open @08:56:55.59 Forearm.seek retract @08:56:56.53 Elbow.reconfigure TightElbow @08:56:56.56 Elbow.seek 1 @08:56:58.16 Elbow.reconfigure LimpElbow @08:56:58.21 Forearm.seek 1 @08:56:59.15 Elbow.hold @08:56:59.20 Elbow.reconfigure TightElbow @08:56:59.23 Hand.release @08:57:00.08 Forearm.coast @08:57:00.11 Forearm.seek 1 + 350 counts @08:57:00.34 Forearm.reconfigure TightForearm @08:57:00.37 Forearm.seek 1 + 800 counts @08:57:00.57 SE.seek down @08:57:01.68
Forearm.reconfigure LooseForearm @08:57:01.71 Forearm.seek retract @08:57:02.28 SE.coast @08:57:02.62
SC.coast @08:57:02.65 PSV.dial :air @08:57:03.70 PS.seek 0.499ml @08:57:09.62 delay 3 seconds @08:57:12.63 PSV.dial :mixloop @08:57:13.06 PRV.dial :air @08:57:13.50 delay 3 seconds @08:57:16.51 PRV.dial CBV @08:57:16.95 CBV.dial AIV @08:57:17.41 PRV.dial between CBV and air @08:57:17.73 PRV.dial :air @08:57:18.08 PS.reconfigure PSslow @08:57:18.11 PS.seek 0.599ml @08:57:20.68 PRV.dial :waste2 @08:57:21.41 PS.reconfigure PSconfig @08:57:21.44 PS.seek empty @08:57:28.50 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:57:28.65 Elbow.seek FlushPuck::Garage @08:57:30.40 Elbow.reconfigure LooseElbow @08:57:30.48 Forearm.seek garage @08:57:31.51 Hand.grip @08:57:32.34 Forearm.seek retract @08:57:33.33 Elbow.reconfigure TightElbow @08:57:33.37 Elbow.seek CC @08:57:36.20 Elbow.reconfigure LooseElbow @08:57:36.29 Forearm.reconfigure TightForearm @08:57:36.33 Forearm.seek CC @08:57:37.43 CC.seek closed @08:57:41.16 Collection Clamp onSpring/offSpring for 5/22 samples, on/off torque 2.07 @08:57:41.21 Hand.release @08:57:42.11 Forearm.reconfigure LooseForearm @08:57:42.14 Forearm.seek retract @08:57:43.18 Clearing PML for modified lysate @08:57:43.19 CTV.dial :air,avoiding => intake PRV.dial :waste2 @08:57:43.20 PTV.dial :PRV,avoiding => puck @08:57:43.93 delay 2 seconds @08:57:45.93 PTV.dial :PRVmixing,avoiding => puck @08:57:46.65 delay 2 seconds @08:57:48.66 PSV.dial :PML @08:57:48.67 delay 2 seconds @08:57:50.67 PRV.dial :air @08:57:51.40 delay 2 seconds @08:57:53.46 PS.seek 1.499ml @08:58:09.59 delay 5 seconds @08:58:14.61 PS.seek 2.500ml @08:58:25.56 PSV.dial :air @08:58:26.02 PS.seek 0.000ml @08:58:52.27 mixing lysate with speDil @08:58:52.28 PSV.dial :PML @08:58:53.31 CBV.dial :PRV @08:58:53.32 PRV.dial :air @08:58:53.33 delay 2 seconds @08:58:55.34 PRV.dial :speDil @08:58:55.77 delay 2 seconds @08:58:57.83 PS.reconfigure PSslow1 @08:58:57.86 PS.seek 0.225ml @08:59:21.15 delay 5 seconds @08:59:26.16 PRV.dial CBV @08:59:26.90 delay 2 seconds @08:59:28.96 PS.seek 0.475ml @08:59:54.71 delay 5 seconds @08:59:59.72 PRV.dial :air @09:00:00.18 delay 2 seconds @09:00:02.19 PS.seek 1.000ml @09:00:55.80 delay 10 seconds @09:01:05.81 PRV.dial CBV @09:01:06.27 CTV.dial :air,avoiding => intake @09:01:06.28 PSV.dial :air @09:01:06.73 PS.reconfigure PSconfig @09:01:06.76 PS.seek 2.500ml @09:01:22.89 delay 3 seconds @09:01:25.90 PSV.dial PTV @09:01:26.61 PTV.dial :PRV,avoiding => puck @09:01:27.34 PS.seek 0.000ml @09:01:53.58 PSV.dial :air @09:01:54.31 PS.seek 2.500ml @09:02:20.55 delay 3 seconds @09:02:23.56 PSV.dial PTV @09:02:24.28 PTV.dial :PRV,avoiding => puck @09:02:24.30 PS.seek 0.000ml @09:02:50.52 Pushing modified lysate into AIV Injection Loop @09:02:50.53 CBV.dial AIV @09:02:50.54 AIV.dial :bypass @09:02:50.55 PRV.dial CBV @09:02:50.56 PSV.dial :air @09:02:51.30 PS.seek 2.500ml @09:03:17.58 delay 3 seconds @09:03:20.59 PSV.dial :PML @09:03:21.03 PTV.dial :PRVmixing,avoiding => puck @09:03:21.81 PS.reconfigure PSslow1 @09:03:21.84 PS.seek 1.740ml @09:04:39.42 delay 5 seconds @09:04:44.43 AIV.dial :sample @09:04:44.96 AWV.select :waste @09:04:45.12 PS.reconfigure PSslow2 @09:04:45.15 PS.seek 1.220ml @09:06:31.07 delay 15 seconds @09:06:46.08 AIV.dial :bypass @09:06:46.63 PS.reconfigure PSconfig @09:06:46.66 PS.seek 0.000ml @09:06:59.98 PSV.dial :air @09:07:00.55 PS.seek 2.500ml @09:07:26.79 PSV.dial :PML @09:07:27.22 PS.seek 0.000ml @09:07:53.54 AIV.dial :coilToMFB @09:07:53.55 AWV.select :air @09:07:53.69 Extracting Nucleic Acid from AIV @09:07:53.71
Beginning samplePassD @09:07:53.72 SPE.hold 2:46:40,55.0C @09:07:53.74 ASV.dial :SPEdelivery @09:07:53.87
AWV.select :air @09:07:53.88 AIV.dial :bypass @09:07:53.89 PSV.dial PSR @09:07:54.28 delay 20 seconds @09:07:54.91
PSR.select :killMFB @09:07:55.01 PS.seek 1.500ml @09:08:11.14 delay 5 seconds @09:08:14.30 primeLysateINT @09:08:14.31 AWV.select :air @09:08:14.32 AIV.dial :coilToMFB @09:08:14.33 ATV.dial :AIV @09:08:15.67 AS.reconfigure ASslow1 @09:08:15.70 AS.seek 0.241ml @09:08:16.15
PSV.dial :waste1 @09:08:16.60 PS.seek 0.000ml @09:08:32.67 PSV.dial PSR @09:08:33.12 PSR.select :air @09:08:33.22 PS.seek 2.500ml @09:08:59.51 PRV.dial CBV @09:08:59.53
CBV.dial PRV @09:08:59.54 PSV.dial :mixloop @09:09:00.57 CTV.dial :air,avoiding => intake @09:09:00.58 PTV.dial :PRVmixing @09:09:00.60 PS.seek 0.000ml @09:09:05.35 delay 10 seconds @09:09:15.36 ATV.dial :air2 @09:09:15.93 AS.seek 0.271ml @09:09:22.29 ATV.dial :waste2 @09:09:22.64 AS.reconfigure ASnorm @09:09:22.67 AS.seek empty @09:09:26.86
PSV.dial PSR @09:09:27.90 PSR.select :flush @09:09:28.00 PS.seek 1.500ml @09:09:36.94 Lysate primed! @09:09:36.95 AFV.select :holdingCoil @09:09:36.96 ATV.dial :air2 @09:09:37.35 AS.reconfigure ASslow1 @09:09:37.38 AS.seek 0.069ml @09:09:44.04
delay 5 seconds @09:09:49.05 PSV.dial :waste1 @09:09:49.49 PS.seek 0.000ml @09:09:51.96 delay 3 seconds @09:09:54.97 ATV.dial :AIV @09:09:55.56 AS.seek 0.469ml @09:10:05.57
PSV.dial PSR @09:10:06.03 PSR.select :air @09:10:06.62 PS.seek 2.500ml @09:10:32.88 PRV.dial CBV @09:10:32.90
CBV.dial PRV @09:10:32.91 PSV.dial :mixloop @09:10:33.93 CTV.dial :air,avoiding => intake @09:10:33.94 PTV.dial :PRVmixing @09:10:33.96 PS.seek 0.000ml @09:11:00.20 PSV.dial PSR @09:11:01.24 PSR.select :flush @09:11:01.34 PS.seek 1.500ml @09:11:17.41 delay 5 seconds @09:11:17.44 delay 5 seconds @09:11:22.43
PSV.dial :waste1 @09:11:22.50 Solid Phase Extraction Heater at 51.6C @09:11:22.51 ATV.dial ASV @09:11:22.83 ASV.dial :SPEdelivery @09:11:22.90
PS.seek 0.000ml @09:11:22.94 AS.seek 0.041ml @09:11:38.94
PSV.dial PSR @09:11:39.40 PSR.select :air @09:11:39.50 PS.seek 2.500ml @09:12:05.72 PRV.dial CBV @09:12:05.74
CBV.dial PRV @09:12:05.75 PSV.dial :mixloop @09:12:06.76 CTV.dial :air,avoiding => intake @09:12:06.77 PTV.dial :PRVmixing @09:12:06.79 PS.seek 0.000ml @09:12:33.00 PSV.dial PSR @09:12:34.02 PSR.select :flush @09:12:34.12 PS.seek 1.500ml @09:12:50.22 delay 5 seconds @09:12:50.49 delay 10 seconds @09:12:55.23
PSV.dial :waste1 @09:12:55.69 PS.seek 0.000ml @09:13:00.50 rinseLysateLineINT @09:13:00.51 AFV.select :carrierSolution @09:13:00.60 delay 2 seconds @09:13:02.61 AS.reconfigure ASconfig @09:13:02.64 AS.seek 1.000ml @09:13:11.75
PSV.dial PSR @09:13:12.21 PSR.select :air @09:13:12.31 PS.seek 2.500ml @09:13:38.50 PRV.dial CBV @09:13:38.52
CBV.dial PRV @09:13:38.53 PSV.dial :mixloop @09:13:39.53 CTV.dial :air,avoiding => intake @09:13:39.54 PTV.dial :PRVmixing @09:13:39.55 PS.reconfigure PSslow @09:13:39.59 PS.seek 1.200ml @09:13:58.81 delay 1 second @09:13:59.82 AFV.select :holdingCoil @09:13:59.91 delay 1 second @09:14:00.92 ATV.dial :AIV @09:14:01.27 AIV.dial :coilToMFB @09:14:01.28 AWV.select :waste @09:14:01.38 AS.reconfigure ASslow0 @09:14:01.42 AS.seek 0.000ml @09:14:06.56
PSV.dial :waste1 @09:14:07.87 PS.reconfigure PSconfig @09:14:07.90 PS.seek 0.000ml @09:14:21.09 PSV.dial :air @09:14:22.12 PS.seek 2.500ml @09:14:48.31 PSV.dial :mixloop @09:14:48.76 PS.reconfigure PSslow @09:14:48.79 PS.seek 0.000ml @09:15:40.12 PSV.dial :air @09:15:40.57 PS.reconfigure PSconfig @09:15:40.60 PS.seek 2.500ml @09:15:43.73 ATV.dial :air2 @09:15:44.30 AS.reconfigure ASslow1 @09:15:44.33 AS.seek 0.050ml @09:15:54.84 ATV.dial :AIV @09:15:55.42 AS.seek 0.010ml @09:16:03.82 ATV.dial :waste @09:16:05.21 AS.seek empty @09:16:06.87
PSV.dial :mixloop @09:16:07.32 PS.reconfigure PSslow @09:16:07.35 PS.seek 0.000ml @09:16:07.67 ethanol wash @09:16:07.68 ATV.dial :air2 @09:16:08.72 AS.reconfigure ASslow2 @09:16:08.75 AS.seek 0.009ml @09:16:19.37 delay 3 seconds @09:16:22.38 Pulling 0.4ml of Ethanol Wash @09:16:22.39 ATV.dial :ethanol @09:16:22.95 AS.reconfigure ASslow1 @09:16:22.98 AS.seek 0.409ml @09:16:58.71
Cleaning Collection Syringe @09:16:58.72 CSV.dial CSR @09:16:59.14 CSR.select :flush @09:16:59.24 CS.seek 4.000ml @09:17:10.18 delay 5 seconds @09:17:15.19 CSR.select :air @09:17:15.28 CS.seek 10.000ml @09:17:31.31 CSV.dial CTV @09:17:31.33
CTV.dial CSV @09:17:32.64 CBV.dial :waste1 @09:17:33.11 CS.seek 0.000ml @09:17:44.79 delay 3 seconds @09:17:47.80 ATV.dial :air1 @09:17:48.15 delay 3 seconds @09:17:51.21 AS.seek 0.434ml @09:17:56.54 delay 3 seconds @09:17:59.21
CSV.dial CSR @09:17:59.55 ATV.dial :ethanol @09:17:59.71
CSR.select :flush @09:17:59.81 CS.seek 4.000ml @09:17:59.95 AS.seek 0.419ml @09:18:03.26 delay 3 seconds @09:18:06.78 ATV.dial ASV @09:18:07.92 AS.seek 0.092ml @09:18:10.71
delay 5 seconds @09:18:15.72 CSR.select :air @09:18:15.81 CS.seek 10.000ml @09:18:31.74 CSV.dial CTV @09:18:31.76
CTV.dial CSV @09:18:32.28 CBV.dial :waste1 @09:18:32.29 CS.seek 0.000ml @09:18:58.48 Garaging FlushPuck in Collection Clamp @09:18:58.53 Elbow.reconfigure TightElbow @09:18:58.57 Elbow.seek CC @09:18:58.81 Elbow.reconfigure LooseElbow @09:18:58.89 Forearm.seek CC @09:18:59.85 CC.seek sealed @09:19:00.77 Hand.grip @09:19:01.46 CC.seek open @09:19:04.78 Forearm.seek retract @09:19:05.72 Elbow.reconfigure TightElbow @09:19:05.75 Elbow.seek garage @09:19:08.54 Elbow.reconfigure LooseElbow @09:19:08.62 Forearm.reconfigure TightForearm @09:19:08.65 Forearm.seek garage @09:19:09.80 Hand.release @09:19:10.62 Forearm.reconfigure LooseForearm @09:19:10.65 Forearm.seek retract @09:19:11.66 Whole Cell Archive sampling 1000ml @09:19:11.68 Loading wcr puck from tube 2 into Collection Clamp @09:19:11.86 SC.reconfigure SCtight @09:19:11.89 SC.seek 2 @09:19:14.12 SC.reconfigure SCloose @09:19:14.30 Elbow.reconfigure TightElbow @09:19:14.34 Elbow.seek 2 @09:19:14.85 delay 3 seconds @09:19:16.20 Forearm.reconfigure TightForearm @09:19:16.23 Forearm.seek 2 + 800 counts @09:19:17.32 SE.reconfigure SEpick @09:19:17.38 SE.seek beamBreak @09:19:17.86 ATV.dial :waste2 @09:19:18.41 AS.reconfigure ASconfig @09:19:18.44 AS.seek empty @09:19:19.30 SE.configure SEconfig @09:19:19.43 Elbow.seek 2 @09:19:19.58 Forearm.seek 2 + 300 counts @09:19:19.93 Forearm.reconfigure LooseForearm @09:19:19.97 Hand.grip @09:19:20.72 SE.seek down @09:19:20.85 Forearm.seek retract @09:19:21.80 Elbow.seek CC @09:19:22.51 SE.coast @09:19:23.32 Elbow.reconfigure LooseElbow @09:19:23.40 Forearm.reconfigure TightForearm @09:19:23.43 Forearm.seek CC @09:19:24.13 ATV.dial :air2 @09:19:24.47 AS.reconfigure ASslow1 @09:19:24.52 AS.seek 1.000ml @09:19:24.66 CC.seek closed @09:19:28.42 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 2.04 @09:19:28.44 Hand.release @09:19:29.24 Forearm.reconfigure LooseForearm @09:19:29.27 Forearm.seek retract @09:19:30.19 SC.coast @09:19:30.22 collectSample 1000ml w/bubble point of 55psi @09:19:30.24 Priming 7ml Sleepy.power :sampler,:ON @09:19:30.25 Intake.select :close @09:19:30.26 Exhaust.select :close @09:19:30.27 SS.hold @09:19:30.44 Calibrating pressure sensors @09:19:31.01 CTV.dial :air @09:19:32.34 SSV.dial :spit,avoiding => exhaust @09:19:32.35 SS.reconfigure SSfast @09:19:32.38 SS.seek 5.00ml @09:19:35.14 SSV.dial CBV @09:19:35.15 CBV.dial SSV @09:19:36.53 delay 3.8 seconds @09:19:40.42 SSV.dial :spit @09:19:41.76 SS.seek 0.00ml @09:19:44.43 SSV.dial CBV @09:19:45.75 CTV.dial :intake @09:19:46.51 Waiting for external sampler to finish priming... @09:19:46.52 delayUntil "09:29:30.24" @09:22:49.08 delay 5 seconds @09:22:54.09 ATV.dial ASV @09:22:54.81 ASV.dial :SPEdelivery @09:22:54.83 AS.seek 0.020ml @09:26:14.97 delay 5 seconds @09:26:19.98 ATV.dial :air2 @09:26:20.74 AS.seek 0.520ml @09:28:03.02 delay 5 seconds @09:28:08.03 ATV.dial ASV @09:28:08.75 ASV.dial :wasteR @09:28:09.35 AS.seek 0.300ml @09:28:54.47 delay 20 seconds @09:29:14.48 AS.seek 0.070ml @09:29:30.25 SSV.dial CBV @09:29:30.27 CBV.dial SSV @09:29:30.28 Allowing 55s for 7.0ml of remaining 7.0ml @09:29:30.29 Intake.select :open @09:29:32.42 Intake @27.4psia, vacuum from 20.0 to 24.0psi @09:29:32.44 SS.reconfigure forSampling @09:29:32.47 SS.seek 7.00ml @09:29:37.72 Stroke completed -- waiting for pressure to equalize ... @09:29:56.77 Intake.select :close @09:29:56.91 SSV.dial :exhaust @09:29:57.69 Exhaust.select :open @09:29:57.79 SS.reconfigure SSexhale @09:29:57.83 SS.seek 0.00ml @09:30:01.64 delay 20 seconds @09:30:01.73 Exhaust.select :close @09:30:01.84 Sampling 1000.0ml @09:30:01.85 Intake.select :close @09:30:01.86 Exhaust.select :close @09:30:01.87 SS.reconfigure SSconfig @09:30:01.90 SS.hold @09:30:02.06 CTV.dial :intake @09:30:02.08 SSV.dial CBV @09:30:02.10 CBV.dial SSV @09:30:02.87 Allowing 185s for 25.0ml of remaining 1000.0ml @09:30:02.88 Intake.select :open @09:30:05.01 Intake @26.6psia, vacuum from 20.0 to 24.0psi @09:30:05.03 SS.reconfigure forSampling @09:30:05.06 SS.seek 25.00ml @09:30:07.68 Vacuum too high -- waiting up to 40s @09:30:09.02 Intake @24.5psia, vacuum from 18.0 to 22.0psi @09:30:09.03 SS.reconfigure forSampling @09:30:09.06 SS.seek 25.00ml @09:30:10.80 Vacuum too high -- waiting up to 40s @09:30:12.80 Intake @25.0psia, vacuum from 18.5 to 22.5psi @09:30:12.82 SS.reconfigure forSampling @09:30:12.85 SS.seek 25.00ml @09:30:14.96 Vacuum too high -- waiting up to 40s @09:30:16.55 Intake @24.9psia, vacuum from 18.4 to 22.4psi @09:30:16.57 SS.reconfigure forSampling @09:30:16.60 SS.seek 25.00ml @09:30:18.36 Vacuum too high -- waiting up to 40s @09:30:19.97 SS.reconfigure forSampling @09:30:20.00 SS.seek 25.00ml @09:30:21.67 Vacuum too high -- waiting up to 40s @09:30:21.68 ATV.dial :waste2 @09:30:22.35 AS.reconfigure ASconfig @09:30:22.40 AS.seek empty @09:30:23.31 Intake @24.8psia, vacuum from 18.3 to 22.3psi @09:30:23.33 SS.reconfigure forSampling @09:30:23.36 SS.seek 25.00ml @09:30:25.46 Vacuum too high -- waiting up to 40s @09:30:26.81 Elution @09:30:26.82 ATV.dial :air1 @09:30:27.26 Intake @25.1psia, vacuum from 18.6 to 22.6psi @09:30:27.28 SS.reconfigure forSampling @09:30:27.31 SS.seek 25.00ml @09:30:27.80 AS.reconfigure ASslow1 @09:30:27.83 AS.seek 0.049ml @09:30:30.17 Vacuum too high -- waiting up to 40s @09:30:31.75 Intake @24.4psia, vacuum from 17.9 to 21.9psi @09:30:31.76 SS.reconfigure forSampling @09:30:31.80 SS.seek 25.00ml @09:30:37.01 Vacuum too high -- waiting up to 40s @09:30:38.34 delay 3 seconds @09:30:38.77 Intake @24.8psia, vacuum from 18.3 to 22.3psi @09:30:38.78 SS.reconfigure forSampling @09:30:38.82 SS.seek 25.00ml @09:30:41.35 ATV.dial :ElutionWater @09:30:41.92 AS.seek 0.109ml @09:30:54.38 delay 3 seconds @09:30:57.39 ATV.dial ASV,via => air2 @09:30:59.04 ASV.dial :SPEelution @09:30:59.15 Stroke completed -- waiting for pressure to equalize ... @09:30:59.54 AFV.select :carrierSolution @09:30:59.68 AS.reconfigure ASnorm @09:30:59.73 AS.seek 0.759ml @09:31:01.65 Intake.select :close @09:31:01.79 SSV.dial :exhaust @09:31:02.54 Exhaust.select :open @09:31:02.64 SS.reconfigure SSexhale @09:31:02.68 SS.seek 0.00ml @09:31:11.34 Exhaust.select :close @09:31:11.44 SSV.dial CBV @09:31:11.46 CBV.dial SSV @09:31:12.23 Allowing 185s for 25.0ml of remaining 975.0ml @09:31:12.24 Intake.select :open @09:31:15.04 Intake @28.0psia, vacuum from 20.0 to 24.0psi @09:31:15.05 SS.reconfigure forSampling @09:31:15.08 SS.seek 25.00ml @09:31:33.27 delay 3 seconds @09:31:36.28 AFV.select :holdingCoil @09:31:36.43 AS.reconfigure ASslow1 @09:31:36.46 AS.seek 0.489ml @09:32:02.48 Vacuum too high -- waiting up to 40s @09:32:02.75 Intake @24.3psia, vacuum from 17.8 to 21.8psi @09:32:02.76 SS.reconfigure forSampling @09:32:02.79 SS.seek 25.00ml @09:32:25.08 Stroke completed -- waiting for pressure to equalize ... @09:32:27.49 Intake.select :close @09:32:27.63 SSV.dial :exhaust @09:32:28.38 Exhaust.select :open @09:32:28.48 SS.reconfigure SSexhale @09:32:28.52 SS.seek 0.00ml @09:32:31.77 delay 5 seconds @09:32:36.83 AS.reconfigure ASslow2 @09:32:36.86 AS.seek 0.454ml @09:32:37.10 Exhaust.select :close @09:32:37.20 SSV.dial CBV @09:32:37.22 CBV.dial SSV @09:32:38.00 Allowing 185s for 25.0ml of remaining 950.0ml @09:32:38.01 Intake.select :open @09:32:40.14 Intake @28.1psia, vacuum from 20.0 to 24.0psi @09:32:40.16 SS.reconfigure forSampling @09:32:40.19 SS.seek 25.00ml @09:33:13.10 delay 1:00 @09:34:13.11 pushing eluate to holding coil @09:34:13.16 AS.seek 0.294ml @09:34:30.53 Stroke completed -- waiting for pressure to equalize ... @09:34:32.35 Intake.select :close @09:34:32.49 SSV.dial :exhaust @09:34:33.25 Exhaust.select :open @09:34:33.35 SS.reconfigure SSexhale @09:34:33.39 SS.seek 0.00ml @09:34:41.98 Exhaust.select :close @09:34:42.08 SSV.dial CBV @09:34:42.10 CBV.dial SSV @09:34:42.87 Allowing 185s for 25.0ml of remaining 925.0ml @09:34:42.88 Intake.select :open @09:34:45.02 Intake @27.4psia, vacuum from 20.0 to 24.0psi @09:34:45.04 SS.reconfigure forSampling @09:34:45.07 SS.seek 25.00ml @09:36:35.46 Stroke completed -- waiting for pressure to equalize ... @09:36:37.92 Intake.select :close @09:36:38.06 SSV.dial :exhaust @09:36:38.93 Exhaust.select :open @09:36:39.03 SS.reconfigure SSexhale @09:36:39.07 SS.seek 0.00ml @09:36:47.76 Exhaust.select :close @09:36:47.86 SSV.dial CBV @09:36:47.88 CBV.dial SSV @09:36:48.65 Allowing 185s for 25.0ml of remaining 900.0ml @09:36:48.66 Intake.select :open @09:36:50.80 Intake @26.7psia, vacuum from 20.0 to 24.0psi @09:36:50.82 SS.reconfigure forSampling @09:36:50.85 SS.seek 25.00ml @09:36:56.47 delay 30 seconds @09:37:26.48 ATV.dial :waste,via => air3 @09:37:28.02 AS.reconfigure ASconfig @09:37:28.05 AS.seek empty @09:37:45.53 Parking Bleach in LysateSource line and SPEcolumn @09:37:45.54 ATV.dial :air3 @09:37:46.11 AS.reconfigure ASslow1 @09:37:46.14 AS.seek 0.029ml @09:37:52.58 ATV.dial :ElutionWater @09:37:53.08 AS.seek 0.059ml @09:37:59.43 ATV.dial :air3 @09:37:59.89 AS.seek 0.069ml @09:38:02.18 delay 2 seconds @09:38:04.19 ATV.dial :bleach @09:38:04.56 AS.seek 0.711ml @09:38:41.18 Stroke completed -- waiting for pressure to equalize ... @09:38:44.15 Intake.select :close @09:38:44.28 SSV.dial :exhaust @09:38:45.03 Exhaust.select :open @09:38:45.13 SS.reconfigure SSexhale @09:38:45.17 SS.seek 0.00ml @09:38:53.69 Exhaust.select :close @09:38:53.79 SSV.dial CBV @09:38:53.81 CBV.dial SSV @09:38:54.58 Allowing 185s for 25.0ml of remaining 875.0ml @09:38:54.59 Intake.select :open @09:38:56.73 Intake @27.3psia, vacuum from 20.0 to 24.0psi @09:38:56.75 SS.reconfigure forSampling @09:38:56.78 SS.seek 25.00ml @09:40:15.74 delay 3 seconds @09:40:18.75 ATV.dial :air3 @09:40:19.16 delay 3 seconds @09:40:22.22 AS.seek 0.736ml @09:40:28.15 delay 3 seconds @09:40:31.16 ATV.dial :bleach @09:40:31.65 AS.seek 0.721ml @09:40:34.95 delay 3 seconds @09:40:37.96 ATV.dial :AIV @09:40:39.06 AS.reconfigure ASconfig @09:40:39.09 AS.seek 0.015ml @09:40:47.14 Stroke completed -- waiting for pressure to equalize ... @09:40:51.91 Intake.select :close @09:40:52.05 SSV.dial :exhaust @09:40:52.81 Exhaust.select :open @09:40:52.91 SS.reconfigure SSexhale @09:40:52.95 SS.seek 0.00ml @09:41:01.57 Exhaust.select :close @09:41:01.67 SSV.dial CBV @09:41:01.69 CBV.dial SSV @09:41:02.47 Allowing 185s for 25.0ml of remaining 850.0ml @09:41:02.48 Intake.select :open @09:41:04.61 Intake @27.3psia, vacuum from 20.0 to 24.0psi @09:41:04.63 SS.reconfigure forSampling @09:41:04.66 SS.seek 25.00ml @09:41:20.54 ATV.dial :waste @09:41:21.91 AS.seek empty @09:41:23.07 ATV.dial :air3 @09:41:23.63 AS.reconfigure ASslow1 @09:41:23.66 AS.seek 0.009ml @09:41:25.99 delay 2 seconds @09:41:28.00 ATV.dial :bleach @09:41:28.37 AS.seek 0.579ml @09:42:55.03 Stroke completed -- waiting for pressure to equalize ... @09:42:59.79 Intake.select :close @09:42:59.93 SSV.dial :exhaust @09:43:00.69 Exhaust.select :open @09:43:00.79 SS.reconfigure SSexhale @09:43:00.83 SS.seek 0.00ml @09:43:09.61 Exhaust.select :close @09:43:09.71 SSV.dial CBV @09:43:09.73 CBV.dial SSV @09:43:10.51 Allowing 185s for 25.0ml of remaining 825.0ml @09:43:10.52 Intake.select :open @09:43:12.65 Intake @28.0psia, vacuum from 20.0 to 24.0psi @09:43:12.67 SS.reconfigure forSampling @09:43:12.70 SS.seek 25.00ml @09:43:24.79 delay 3 seconds @09:43:27.80 ATV.dial :air3 @09:43:28.22 delay 3 seconds @09:43:31.28 AS.seek 0.604ml @09:43:36.67 delay 3 seconds @09:43:39.68 ATV.dial :bleach @09:43:40.17 AS.seek 0.589ml @09:43:43.47 delay 3 seconds @09:43:46.48 ATV.dial :ASV @09:43:47.39 ASV.dial :wasteR @09:43:48.05 AS.seek 0.000ml @09:45:03.40 Stroke completed -- waiting for pressure to equalize ... @09:45:08.76 Intake.select :close @09:45:08.90 SSV.dial :exhaust @09:45:09.65 Exhaust.select :open @09:45:09.75 SS.reconfigure SSexhale @09:45:09.79 SS.seek 0.00ml @09:45:18.38 Exhaust.select :close @09:45:18.48 SSV.dial CBV @09:45:18.50 CBV.dial SSV @09:45:19.28 Allowing 185s for 25.0ml of remaining 800.0ml @09:45:19.29 Intake.select :open @09:45:21.42 Intake @35.1psia, vacuum from 20.0 to 24.0psi @09:45:21.44 SS.reconfigure forSampling @09:45:21.47 SS.seek 25.00ml @09:45:48.45 delay 1:00 @09:46:48.46 AFV.select :carrierSolution @09:46:48.55 delay 2 seconds @09:46:50.56 AS.reconfigure ASconfig @09:46:50.59 AS.seek 1.000ml @09:47:11.82 Stroke completed -- waiting for pressure to equalize ... @09:47:15.39 Intake.select :close @09:47:15.53 SSV.dial :exhaust @09:47:16.92 Exhaust.select :open @09:47:17.02 SS.reconfigure SSexhale @09:47:17.06 SS.seek 0.00ml @09:47:25.66 Exhaust.select :close @09:47:25.76 SSV.dial CBV @09:47:25.78 CBV.dial SSV @09:47:26.55 Allowing 185s for 25.0ml of remaining 775.0ml @09:47:26.57 Intake.select :open @09:47:28.71 Intake @36.3psia, vacuum from 20.0 to 24.0psi @09:47:28.72 SS.reconfigure forSampling @09:47:28.75 SS.seek 25.00ml @09:47:49.19 delay 1 second @09:47:50.20 AFV.select :holdingCoil @09:47:50.30 delay 1 second @09:47:51.31 ATV.dial :ASV @09:47:51.32 ASV.dial :wasteR @09:47:51.33 AS.reconfigure ASslow1 @09:47:51.37 AS.seek 0.000ml @09:48:26.47 Vacuum too high -- waiting up to 40s @09:48:26.95 Intake @35.0psia, vacuum from 20.0 to 24.0psi @09:48:26.97 SS.reconfigure forSampling @09:48:27.00 SS.seek 25.00ml @09:48:50.60 Vacuum too high -- waiting up to 40s @09:48:51.52 Intake @35.4psia, vacuum from 20.0 to 24.0psi @09:48:51.54 SS.reconfigure forSampling @09:48:51.57 SS.seek 25.00ml @09:49:02.13 Vacuum too high -- waiting up to 40s @09:49:02.83 Intake @35.1psia, vacuum from 20.0 to 24.0psi @09:49:02.85 SS.reconfigure forSampling @09:49:02.88 SS.seek 25.00ml @09:49:11.10 Vacuum too high -- waiting up to 40s @09:49:12.01 Intake @35.2psia, vacuum from 20.0 to 24.0psi @09:49:12.03 SS.reconfigure forSampling @09:49:12.06 SS.seek 25.00ml @09:49:18.15 Vacuum too high -- waiting up to 40s @09:49:18.86 Intake @34.4psia, vacuum from 20.0 to 24.0psi @09:49:18.88 SS.reconfigure forSampling @09:49:18.91 SS.seek 25.00ml @09:49:24.06 Stroke completed -- waiting for pressure to equalize ... @09:49:29.41 Intake.select :close @09:49:29.55 SSV.dial :exhaust @09:49:30.31 Exhaust.select :open @09:49:30.41 SS.reconfigure SSexhale @09:49:30.45 SS.seek 0.00ml @09:49:39.07 Exhaust.select :close @09:49:39.17 SSV.dial CBV @09:49:39.19 CBV.dial SSV @09:49:39.96 Allowing 185s for 25.0ml of remaining 750.0ml @09:49:39.97 Intake.select :open @09:49:42.11 Intake @35.1psia, vacuum from 20.0 to 24.0psi @09:49:42.13 SS.reconfigure forSampling @09:49:42.16 SS.seek 25.00ml @09:49:52.08 Vacuum too high -- waiting up to 40s @09:49:52.35 Intake @31.4psia, vacuum from 20.0 to 24.0psi @09:49:52.37 SS.reconfigure forSampling @09:49:52.40 SS.seek 25.00ml @09:51:15.61 delay 20 seconds @09:51:32.94 Stroke completed -- waiting for pressure to equalize ... @09:51:35.62 ATV.dial :air3 @09:51:36.77 AS.seek 0.590ml @09:51:40.08 Intake.select :close @09:51:40.21 SSV.dial :exhaust @09:51:40.97 Exhaust.select :open @09:51:41.07 SS.reconfigure SSexhale @09:51:41.11 SS.seek 0.00ml @09:51:49.68 Exhaust.select :close @09:51:49.78 SSV.dial CBV @09:51:49.80 CBV.dial SSV @09:51:51.19 Allowing 185s for 25.0ml of remaining 725.0ml @09:51:51.20 Intake.select :open @09:51:53.33 Intake @34.9psia, vacuum from 20.0 to 24.0psi @09:51:53.35 SS.reconfigure forSampling @09:51:53.38 SS.seek 25.00ml @09:52:03.77 Vacuum too high -- waiting up to 40s @09:52:04.49 Intake @34.4psia, vacuum from 20.0 to 24.0psi @09:52:04.50 SS.reconfigure forSampling @09:52:04.53 SS.seek 25.00ml @09:52:14.52 Vacuum too high -- waiting up to 40s @09:52:15.45 Intake @34.1psia, vacuum from 20.0 to 24.0psi @09:52:15.47 SS.reconfigure forSampling @09:52:15.50 SS.seek 25.00ml @09:52:25.21 Vacuum too high -- waiting up to 40s @09:52:26.13 Intake @33.8psia, vacuum from 20.0 to 24.0psi @09:52:26.15 SS.reconfigure forSampling @09:52:26.18 SS.seek 25.00ml @09:52:35.52 Vacuum too high -- waiting up to 40s @09:52:36.66 Intake @33.8psia, vacuum from 20.0 to 24.0psi @09:52:36.68 SS.reconfigure forSampling @09:52:36.71 SS.seek 25.00ml @09:52:44.95 Vacuum too high -- waiting up to 40s @09:52:46.09 Intake @33.6psia, vacuum from 20.0 to 24.0psi @09:52:46.10 SS.reconfigure forSampling @09:52:46.14 SS.seek 25.00ml @09:52:52.89 Vacuum too high -- waiting up to 40s @09:52:54.03 Intake @33.1psia, vacuum from 20.0 to 24.0psi @09:52:54.05 SS.reconfigure forSampling @09:52:54.08 SS.seek 25.00ml @09:53:02.33 Vacuum too high -- waiting up to 40s @09:53:03.68 SS.seek 25.00ml @09:53:11.86 Vacuum too high -- waiting up to 40s @09:53:13.19 Intake @33.2psia, vacuum from 20.0 to 24.0psi @09:53:13.21 SS.reconfigure forSampling @09:53:13.24 SS.seek 25.00ml @09:53:20.11 Vacuum too high -- waiting up to 40s @09:53:21.68 Intake @33.9psia, vacuum from 20.0 to 24.0psi @09:53:21.70 SS.reconfigure forSampling @09:53:21.73 SS.seek 25.00ml @09:53:27.07 Vacuum too high -- waiting up to 40s @09:53:28.19 Intake @33.1psia, vacuum from 20.0 to 24.0psi @09:53:28.20 SS.reconfigure forSampling @09:53:28.24 SS.seek 25.00ml @09:53:36.51 Vacuum too high -- waiting up to 40s @09:53:37.42 delay 3 seconds @09:53:38.75 Intake @34.3psia, vacuum from 20.0 to 24.0psi @09:53:38.76 SS.reconfigure forSampling @09:53:38.80 SS.seek 25.00ml @09:53:40.43 ATV.dial :ASV @09:53:41.62 ASV.dial :wasteR @09:53:41.69 AS.seek 0.090ml @09:53:45.74 Vacuum too high -- waiting up to 40s @09:53:47.10 Intake @35.1psia, vacuum from 20.0 to 24.0psi @09:53:47.12 SS.reconfigure forSampling @09:53:47.15 SS.seek 25.00ml @09:53:52.08 Vacuum too high -- waiting up to 40s @09:53:53.22 Intake @34.4psia, vacuum from 20.0 to 24.0psi @09:53:53.24 SS.reconfigure forSampling @09:53:53.27 SS.seek 25.00ml @09:53:58.46 Vacuum too high -- waiting up to 40s @09:53:59.82 Intake @35.0psia, vacuum from 20.0 to 24.0psi @09:53:59.84 SS.reconfigure forSampling @09:53:59.87 SS.seek 25.00ml @09:54:04.61 Vacuum too high -- waiting up to 40s @09:54:05.74 Intake @34.4psia, vacuum from 20.0 to 24.0psi @09:54:05.76 SS.reconfigure forSampling @09:54:05.79 SS.seek 25.00ml @09:54:05.84 Stroke completed -- waiting for pressure to equalize ... @09:54:11.78 Intake.select :close @09:54:11.92 SSV.dial :exhaust @09:54:12.67 Exhaust.select :open @09:54:12.77 SS.reconfigure SSexhale @09:54:12.81 SS.seek 0.00ml @09:54:21.54 Exhaust.select :close @09:54:21.64 SSV.dial CBV @09:54:21.66 CBV.dial SSV @09:54:22.43 Allowing 185s for 25.0ml of remaining 700.0ml @09:54:22.44 Intake.select :open @09:54:24.58 Intake @35.1psia, vacuum from 20.0 to 24.0psi @09:54:24.60 SS.reconfigure forSampling @09:54:24.63 SS.seek 25.00ml @09:54:31.24 Vacuum too high -- waiting up to 40s @09:54:32.15 Intake @34.6psia, vacuum from 20.0 to 24.0psi @09:54:32.16 SS.reconfigure forSampling @09:54:32.20 SS.seek 25.00ml @09:54:37.20 Vacuum too high -- waiting up to 40s @09:54:38.11 Intake @33.6psia, vacuum from 20.0 to 24.0psi @09:54:38.12 SS.reconfigure forSampling @09:54:38.16 SS.seek 25.00ml @09:54:43.13 Vacuum too high -- waiting up to 40s @09:54:44.03 Intake @32.0psia, vacuum from 20.0 to 24.0psi @09:54:44.04 SS.reconfigure forSampling @09:54:44.08 SS.seek 25.00ml @09:54:50.23 Vacuum too high -- waiting up to 40s @09:54:51.57 Intake @32.4psia, vacuum from 20.0 to 24.0psi @09:54:51.58 SS.reconfigure forSampling @09:54:51.62 SS.seek 25.00ml @09:54:58.64 Vacuum too high -- waiting up to 40s @09:54:59.88 Intake @30.9psia, vacuum from 20.0 to 24.0psi @09:54:59.90 SS.reconfigure forSampling @09:54:59.93 SS.seek 25.00ml @09:55:07.55 Vacuum too high -- waiting up to 40s @09:55:09.35 Intake @30.7psia, vacuum from 20.0 to 24.0psi @09:55:09.37 SS.reconfigure forSampling @09:55:09.40 SS.seek 25.00ml @09:55:16.70 Vacuum too high -- waiting up to 40s @09:55:18.50 Intake @29.6psia, vacuum from 20.0 to 24.0psi @09:55:18.51 SS.reconfigure forSampling @09:55:18.55 SS.seek 25.00ml @09:55:23.91 delay 30 seconds @09:55:26.67 Vacuum too high -- waiting up to 40s @09:55:29.76 Intake @31.7psia, vacuum from 20.0 to 24.0psi @09:55:29.77 SS.reconfigure forSampling @09:55:29.81 SS.seek 25.00ml @09:55:35.19 Vacuum too high -- waiting up to 40s @09:55:36.30 Intake @29.3psia, vacuum from 20.0 to 24.0psi @09:55:36.31 SS.reconfigure forSampling @09:55:36.35 SS.seek 25.00ml @09:55:46.04 Vacuum too high -- waiting up to 40s @09:55:48.89 Intake @28.7psia, vacuum from 20.0 to 24.0psi @09:55:48.91 SS.reconfigure forSampling @09:55:48.94 SS.seek 25.00ml @09:55:53.92 ATV.dial :extSrc @09:55:54.43 delay 3 seconds @09:55:57.44 ATV.dial :ElutionWater @09:55:58.38 AS.seek 0.120ml @09:56:01.23 Vacuum too high -- waiting up to 40s @09:56:04.68 ATV.dial :air3 @09:56:05.88 AS.seek 0.130ml @09:56:06.06 Intake @29.9psia, vacuum from 20.0 to 24.0psi @09:56:06.07 SS.reconfigure forSampling @09:56:06.10 SS.seek 25.00ml @09:56:08.21 delay 2 seconds @09:56:10.22 ATV.dial :bleach @09:56:10.60 AS.seek 0.230ml @09:56:15.48 Vacuum too high -- waiting up to 40s @09:56:18.38 Intake @28.7psia, vacuum from 20.0 to 24.0psi @09:56:18.40 SS.reconfigure forSampling @09:56:18.43 SS.seek 25.00ml @09:56:30.82 Vacuum too high -- waiting up to 40s @09:56:31.27 delay 3 seconds @09:56:34.31 ATV.dial :air3 @09:56:34.67 delay 3 seconds @09:56:35.47 Intake @28.2psia, vacuum from 20.0 to 24.0psi @09:56:35.49 SS.reconfigure forSampling @09:56:35.52 SS.seek 25.00ml @09:56:37.72 AS.seek 0.255ml @09:56:43.07 delay 3 seconds @09:56:46.08 ATV.dial :bleach @09:56:46.48 AS.seek 0.240ml @09:56:47.32 Stroke completed -- waiting for pressure to equalize ... @09:56:49.80 delay 3 seconds @09:56:52.81 ATV.dial :ASV @09:56:53.79 ASV.dial :SPEdelivery @09:56:54.27 AFV.select :carrierSolution @09:56:54.41 delay 2 seconds @09:56:56.42 AS.reconfigure ASconfig @09:56:56.46 AS.seek 0.500ml @09:57:04.96 Intake.select :close @09:57:05.10 SSV.dial :exhaust @09:57:06.58 Exhaust.select :open @09:57:06.68 SS.reconfigure SSexhale @09:57:06.72 SS.seek 0.00ml @09:57:11.89 delay 1 second @09:57:12.90 AFV.select :holdingCoil @09:57:13.04 AS.reconfigure ASslow1 @09:57:13.07 AS.seek 0.170ml @09:57:15.66 Exhaust.select :close @09:57:15.76 SSV.dial CBV @09:57:15.78 CBV.dial SSV @09:57:16.56 Allowing 185s for 25.0ml of remaining 675.0ml @09:57:16.57 Intake.select :open @09:57:18.71 Intake @25.3psia, vacuum from 18.8 to 22.8psi @09:57:18.72 SS.reconfigure forSampling @09:57:18.75 SS.seek 25.00ml @09:57:37.98 Vacuum too high -- waiting up to 40s @09:57:42.18 Intake @24.8psia, vacuum from 18.3 to 22.3psi @09:57:42.20 SS.seek 25.00ml @09:57:53.79 Vacuum too high -- waiting up to 40s @09:57:58.38 Intake @22.8psia, vacuum from 16.3 to 20.3psi @09:57:58.40 SS.seek 25.00ml @09:58:07.00 Vacuum too high -- waiting up to 40s @09:58:15.78 Intake @22.5psia, vacuum from 16.0 to 20.0psi @09:58:15.80 SS.seek 25.00ml @09:58:20.66 SPE.hold 2:46:40,95.0C @09:58:20.68 delay 3:00 @09:58:28.51 Vacuum too high -- waiting up to 40s @09:58:36.88 Intake @21.2psia, vacuum from 14.7 to 18.7psi @09:58:36.89 SS.seek 25.00ml @09:58:46.00 Vacuum too high -- waiting up to 40s @09:58:57.31 Intake @20.4psia, vacuum from 13.9 to 17.9psi @09:58:57.33 SS.seek 25.00ml @09:59:09.72 Vacuum too high -- waiting up to 40s @09:59:23.11 Intake @19.5psia, vacuum from 13.0 to 17.0psi @09:59:23.13 SS.seek 25.00ml @09:59:35.82 Vacuum too high -- waiting up to 40s @09:59:53.02 Intake @19.1psia, vacuum from 12.6 to 16.6psi @09:59:53.03 SS.seek 25.00ml @10:00:07.66 Vacuum too high -- waiting up to 40s @10:00:21.57 SS.reconfigure SSconfig @10:00:21.61 SS.hold @10:00:21.70 Intake.select :close @10:00:21.86 ShallowSampler::Clogged in WCR -- progress too slow after 347.7ml @10:00:21.87 SSV.dial :exhaust @10:00:22.68 Exhaust.select :open @10:00:22.78 SS.reconfigure SSexhale @10:00:22.82 SS.seek 0.00ml @10:00:31.35 Exhaust.select :close @10:00:31.46 Sampled 347.7ml @10:00:31.47 collectSample: Complete @10:00:31.48 Drying puck before fixing for 04:00.00 @10:00:31.49 CBV.dial :waste2b @10:00:32.30 delay 1:00 @10:00:34.45 Sleepy.power :sampler,:OFF @10:01:20.70 AFV.select :carrierSolution @10:01:20.79 delay 2 seconds @10:01:22.80 AS.reconfigure ASconfig @10:01:22.83 AS.seek 1.000ml @10:01:32.30 CSV.dial :air @10:01:32.75 CS.reconfigure CSfast @10:01:32.78 CS.seek 10.000ml @10:01:57.38 CBV.dial between CSV and waste2b @10:01:57.72 Pumping wcr puck from tube 2 up to 55psi... @10:01:57.77 CBV.dial SSV @10:01:59.21 CBV.dial between CSV and waste2b @10:02:00.18 CSV.dial between air and CTV @10:02:00.58 CS.seek 6.350ml @10:02:09.92 CTV.dial CSV,avoiding => air @10:02:09.94 CSV.dial CTV @10:02:10.72 CS.reconfigure CSpressurize @10:02:10.75 CS.seek 0.000ml @10:02:11.47 delay 1 second @10:02:12.48 AFV.select :holdingCoil @10:02:12.57 delay 1 second @10:02:13.58 ASV.dial :wasteD @10:02:14.87 AS.seek 0.000ml @10:02:27.38 CSV.dial :air @10:02:27.92 CS.reconfigure CSfast @10:02:27.95 CS.seek 10.000ml @10:02:51.82 CSV.dial between air and CTV @10:02:52.21 CS.seek 1.974ml @10:03:11.64 CTV.dial CSV,avoiding => air @10:03:11.65 CSV.dial CTV @10:03:11.95 CS.reconfigure CSpressurize @10:03:11.98 CS.seek 0.000ml @10:03:12.03 CBV.dial :waste2b @10:03:12.35 CS.reconfigure CSconfig @10:03:12.38 CS.seek empty @10:03:13.47 Priming Eluate @10:03:13.48 AFV.select :carrierSolution @10:03:13.57 delay 2 seconds @10:03:15.58 AS.seek 0.500ml @10:03:18.25 delay 1:00 @10:03:45.02 delay 1 second @10:03:46.03 AFV.select :holdingCoil @10:03:46.12 ATV.dial ASV @10:03:46.13 ASV.dial :SPErecovery @10:03:46.63 Swish #1 of 2 @10:03:46.69 AS.reconfigure ASslow0 @10:03:46.72 AS.seek 0.600ml @10:03:57.24 AS.seek 0.500ml @10:04:07.72 Swish #2 of 2 @10:04:07.78 AS.seek 0.600ml @10:04:18.26 CTV.dial :air,avoiding => intake @10:04:18.36 AS.seek 0.500ml @10:04:19.54 Fix #1 of 2 (with rnal) for 02:00.00 @10:04:19.55 CS Pulling 2ml of rnal @10:04:19.56 CSV.dial CSR @10:04:20.20 CSR.select :rnal @10:04:20.30 CS.seek 2.000ml @10:04:26.25 delay 5 seconds @10:04:28.86 delay 3 seconds @10:04:31.26 CSR.select :air @10:04:31.36 CS.reconfigure CSfast @10:04:31.40 CS.seek 10.000ml @10:04:31.92 AS.reconfigure ASslow1 @10:04:31.95 AS.seek 0.676ml @10:04:50.65 CBV.dial between CSV and waste2b @10:04:50.97 Pumping wcr puck from tube 2 up to 55psi... @10:04:51.01 CBV.dial SSV @10:04:53.04 CBV.dial between CSV and waste2b @10:04:53.97 CSV.dial between air and CTV @10:04:54.55 CS.seek 6.490ml @10:05:03.51 CTV.dial CSV,avoiding => air @10:05:03.53 CSV.dial CTV @10:05:04.86 CS.reconfigure CSpressurize @10:05:04.89 CS.seek 0.000ml @10:05:08.11 delay 3 seconds @10:05:11.12 Eluate primed @10:05:11.13 ATV.dial :waste2 @10:05:11.71 AS.reconfigure ASnorm @10:05:11.79 AS.seek 0.000ml @10:05:21.45 delay 2:00 @10:05:46.64 SPE.coast @10:05:46.72 ASV.dial :SPErecovery @10:05:46.73 Setting up PCR for HF183em -- writing /var/log/esp/HF183em14feb1610h248ml.pcr @10:05:46.74 Building PCR Slug from ASV with MM2 mix, PP4 primer @10:05:46.75 Priming with carrier @10:05:46.76 ATV.dial ARV @10:05:47.47 ARV.dial :carrier @10:05:47.77 AS.seek 0.500ml @10:06:13.65 AFV.select :carrierSolution @10:06:13.75 delay 2 seconds @10:06:15.76 AS.reconfigure ASconfig @10:06:15.80 AS.seek 1.000ml @10:06:45.21 delay 1 second @10:06:46.22 AFV.select :holdingCoil @10:06:46.32 delay 1 second @10:06:47.33 ATV.dial :waste @10:06:48.60 AS.reconfigure ASnorm @10:06:48.63 AS.seek empty @10:07:21.46 CBV.dial :waste2b @10:07:21.78 CS.reconfigure CSconfig @10:07:21.81 CS.seek empty @10:07:22.80 delay 1:00 @10:07:40.10 AFV.select :carrierSolution @10:07:40.21 delay 2 seconds @10:07:42.22 AS.reconfigure ASconfig @10:07:42.25 AS.seek 0.500ml @10:08:11.73 delay 1 second @10:08:12.74 AFV.select :holdingCoil @10:08:12.84 Building Slug @10:08:12.85 ATV.dial :ARV @10:08:14.12 delay 2 seconds @10:08:16.13 ARV.dial :air1 @10:08:16.46 delay 2 seconds @10:08:18.52 AS.reconfigure ASslow2 @10:08:18.55 AS.seek 0.506ml @10:08:22.81 CTV.dial :air,avoiding => intake @10:08:24.09 Fix #2 of 2 (with rnal) for 02:00.00 @10:08:24.10 CS Pulling 2ml of rnal CSV.dial CSR @10:08:24.52 CSR.select :rnal @10:08:24.62 CS.seek 2.000ml @10:08:24.87 delay 2 seconds @10:08:26.88 ARV.dial :MM2 @10:08:28.12 delay 2 seconds @10:08:30.13 Adding MM2 @10:08:30.18 AS.seek 0.524ml @10:08:30.52 delay 5 seconds @10:08:35.53 CSR.select :air @10:08:35.62 CS.reconfigure CSfast @10:08:35.66 CS.seek 10.000ml @10:08:48.91 delay 25 seconds @10:08:54.55 CBV.dial between CSV and waste2b @10:08:54.89 Pumping wcr puck from tube 2 up to 55psi... @10:08:54.93 CBV.dial SSV @10:08:56.36 CBV.dial between CSV and waste2b @10:08:57.29 CSV.dial between air and CTV @10:08:58.35 CS.seek 6.392ml @10:09:07.37 CTV.dial CSV,avoiding => air @10:09:07.39 CSV.dial CTV @10:09:08.70 CS.reconfigure CSpressurize @10:09:08.73 CS.seek 0.000ml @10:09:13.92 ARV.dial :PP4 @10:09:14.26 Adding PP4 @10:09:14.27 delay 2 seconds @10:09:16.33 AS.seek 0.530ml @10:09:22.63 delay 10 seconds @10:09:24.91 delay 2:00 @10:09:32.64 ARV.dial :air3 @10:09:33.07 adding ARV air @10:09:33.08 delay 2 seconds @10:09:35.14 AS.seek 0.536ml @10:09:41.38 delay 5 seconds @10:09:46.39 ARV.dial :carrier @10:09:47.44 delay 7 seconds @10:09:54.50 AS.seek 0.594ml @10:10:53.86 delay 5 seconds @10:10:58.87 ATV.dial :ASV @10:10:59.28 delay 2 seconds @10:11:01.34 AS.seek 0.600ml @10:11:07.77 delay 4 seconds @10:11:11.78 ATV.dial ARV @10:11:12.21 delay 2 seconds @10:11:14.22 ARV.dial :carrier @10:11:14.23 delay 2 seconds @10:11:16.28 AS.seek 0.650ml @10:11:24.92 CBV.dial :waste2b @10:11:25.24 CS.reconfigure CSconfig @10:11:25.27 CS.seek empty @10:11:26.45 delay 1:00 @10:12:07.42 delay 4 seconds @10:12:11.98 ATV.dial PCR @10:12:12.50 ASV.dial :waste1 @10:12:13.41 ARV.dial :air1 @10:12:13.76 Positioning Slug by 0.324ml @10:12:13.81 AS.seek 0.326ml @10:12:26.46 CTV.dial :air,avoiding => intake @10:12:27.76 Storing wcr puck in Collection Clamp to tube 1 @10:12:27.90 SC.reconfigure SCtight @10:12:27.93 SC.seek 1 @10:12:30.17 SC.reconfigure SCloose @10:12:30.35 Elbow.reconfigure TightElbow @10:12:30.38 Elbow.seek 1 @10:12:32.06 Forearm.reconfigure TightForearm @10:12:32.09 Forearm.seek 1 + 800 counts @10:12:33.23 SE.reconfigure SEpick @10:12:33.28 SE.seek beamBreak @10:12:35.21 SE.configure SEconfig @10:12:35.33 Elbow.reconfigure LimpElbow @10:12:35.37 Forearm.reconfigure LooseForearm @10:12:35.41 Forearm.seek 1 @10:12:35.62 Elbow.hold @10:12:35.68 Elbow.reconfigure TightElbow @10:12:35.76 SE.seek 10.05 Pucks below Top Plate @10:12:36.11 Forearm.coast @10:12:36.15 Forearm.seek 1 + 339 counts @10:12:36.39 Forearm.reconfigure TightForearm @10:12:36.43 Forearm.seek 1 + 800 counts @10:12:36.71 SE.seek 11.05 Pucks below Top Plate @10:12:37.24 Forearm.reconfigure LooseForearm @10:12:37.27 Forearm.seek retract @10:12:38.19 Elbow.seek CC @10:12:39.75 Elbow.reconfigure LooseElbow @10:12:39.83 Forearm.seek CC @10:12:40.75 CC.seek sealed @10:12:41.69 Hand.grip @10:12:42.47 CC.seek open @10:12:45.83 Forearm.seek retract @10:12:46.78 Elbow.reconfigure TightElbow @10:12:46.81 Elbow.seek 1 @10:12:48.41 Elbow.reconfigure LimpElbow @10:12:48.46 Forearm.seek 1 @10:12:49.46 Elbow.hold @10:12:49.51 Elbow.reconfigure TightElbow @10:12:49.55 Hand.release @10:12:50.43 Forearm.coast @10:12:50.47 Forearm.seek 1 + 314 counts @10:12:50.72 Forearm.reconfigure TightForearm @10:12:50.75 Forearm.seek 1 + 800 counts @10:12:50.96 SE.seek down @10:12:52.07 Forearm.reconfigure LooseForearm @10:12:52.10 Forearm.seek retract @10:12:52.72 SE.coast @10:12:53.01 SC.coast @10:12:53.04 Starting finalPurge @10:12:53.05 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @10:12:53.20 Elbow.seek FlushPuck::Garage @10:12:55.03 Elbow.reconfigure LooseElbow @10:12:55.76 Forearm.seek garage @10:12:56.74 Hand.grip @10:12:57.56 Forearm.seek retract @10:12:58.60 Elbow.reconfigure TightElbow @10:12:58.63 Elbow.seek CC @10:13:01.37 Elbow.reconfigure LooseElbow @10:13:01.46 Forearm.reconfigure TightForearm @10:13:01.50 Forearm.seek CC @10:13:02.64 CC.seek closed @10:13:06.44 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.99 @10:13:06.49 Hand.release @10:13:07.26 Forearm.reconfigure LooseForearm @10:13:07.29 Forearm.seek retract @10:13:08.27 Flushing the Collection Valve::Manifold @10:13:08.37 CS Pulling 5ml of flush CSV.dial CSR @10:13:08.80 CSR.select :flush @10:13:08.90 CS.seek 5.000ml @10:13:22.47 delay 5 seconds @10:13:27.48 CSR.select :air @10:13:27.62 CS.reconfigure CSfast @10:13:27.65 CS.seek 7.500ml @10:13:34.19 CSV.dial :waste @10:13:35.45 CS.reconfigure CSconfig @10:13:35.48 CS.seek 0.000ml @10:13:55.25 Flushing lower manifold's waste1 port @10:13:55.34 CSV.dial :flush @10:13:55.79 CS.reconfigure CSfast @10:13:55.82 CS.seek 5.000ml @10:14:07.88 delay 5 seconds @10:14:12.89 CSV.dial :air @10:14:13.36 CS.seek 7.000ml @10:14:18.72 CSV.dial CTV @10:14:18.74
CTV.dial CSV @10:14:20.08 CBV.dial :waste1 @10:14:20.82 CS.seek 0.000ml @10:14:37.38 Flushing lower manifold's waste2b port @10:14:37.47 CSV.dial :flush @10:14:38.28 CS.seek 5.000ml @10:14:50.38 delay 5 seconds @10:14:55.39 CSV.dial :air @10:14:55.94 CS.seek 7.000ml @10:15:01.31 CSV.dial CTV @10:15:01.34
CTV.dial CSV @10:15:01.98 CBV.dial :waste2b @10:15:02.73 CS.seek 0.000ml @10:15:19.31 Flushing upper manifold @10:15:19.40 CSV.dial :flush @10:15:20.12 CS.seek 5.000ml @10:15:32.28 delay 5 seconds @10:15:37.29 CSV.dial :air @10:15:37.84 CS.seek 7.000ml @10:15:43.31 CSV.dial CBV @10:15:43.33
CBV.dial CSV @10:15:44.45 CTV.dial :air @10:15:45.73 CS.seek 0.000ml @10:16:02.31 delay 7 seconds @10:16:09.32 CS.seek 3.000ml @10:16:16.90 CSV.dial :waste @10:16:17.41 CS.seek 0.000ml @10:16:25.09 CSV.dial :flush @10:16:25.53 CS.seek 3.000ml @10:16:33.13 delay 5 seconds @10:16:38.14 CSV.dial :air @10:16:38.63 CS.seek 5.000ml @10:16:44.07 CSV.dial :air @10:16:44.09 CS.seek 0.000ml @10:16:56.16 delay 7 seconds @10:17:03.17 CS.seek 3.000ml @10:17:10.79 CSV.dial :waste @10:17:12.22 CS.seek 0.000ml @10:17:19.85 Disinfecting external Intake with mfbkill @10:17:19.94 CSV.dial CSR @10:17:21.29 CSR.select :mfbkill @10:17:21.39 CS.seek 8.000ml @10:17:40.16 delay 5 seconds @10:17:44.55 delay 5 seconds @10:17:45.17
CSR.select :air @10:17:45.26 CS.seek 10.000ml @10:17:49.56 ATV.dial ARV @10:17:50.08 ASV.dial :PCRlock @10:17:50.60 CSV.dial CBV @10:17:50.62
CBV.dial CSV @10:17:50.80 delay 5 seconds @10:17:51.62
CTV.dial :intake @10:17:52.32 Intake.select :open @10:17:52.42 CS.seek 2.000ml @10:17:55.81 Running HF183em ProfileEM60C2 thermocycling @10:17:55.82 PCR.program ProfileEM60C2 @10:17:55.93 ProfileEM60C2 should complete in < 2:11:38 @10:17:55.94 PCR.run 2:49:32.5 @10:18:11.66
Intake.select :close @10:18:11.75 CTV.dial :air @10:18:12.51 CS.reconfigure CSconfig @10:18:12.54 CS.seek 0.000ml @10:18:18.41 Flushing lower manifold's waste1 port @10:18:18.42 CS Pulling 4ml of mfbkill @10:18:18.43 CSV.dial CSR @10:18:19.49 CSR.select :mfbkill @10:18:19.59 CS.seek 4.000ml @10:18:30.50 delay 5 seconds @10:18:35.51 CSR.select :air @10:18:35.60 CS.reconfigure CSfast @10:18:35.64 CS.seek 10.000ml @10:18:49.95 CSV.dial CTV @10:18:49.97
CTV.dial CSV @10:18:51.31 CBV.dial :waste1 @10:18:51.78 CS.seek 0.000ml @10:19:15.25 Flushing lower manifold's waste2b port @10:19:15.26 CS Pulling 4ml of mfbkill @10:19:15.27 CSV.dial CSR @10:19:15.76 CSR.select :mfbkill @10:19:15.86 CS.reconfigure CSconfig @10:19:15.90 CS.seek 4.000ml @10:19:26.84 delay 5 seconds @10:19:31.85 CSR.select :air @10:19:31.94 CS.reconfigure CSfast @10:19:31.98 CS.seek 10.000ml @10:19:46.32 CSV.dial CTV @10:19:46.34
CTV.dial CSV @10:19:46.85 CBV.dial :waste2b @10:19:47.59 CS.seek 0.000ml @10:20:10.90 Killing upper manifold @10:20:10.91 CS Pulling 4ml of mfbkill @10:20:10.92 CSV.dial CSR @10:20:11.42 CSR.select :mfbkill @10:20:11.52 CS.reconfigure CSconfig @10:20:11.56 CS.seek 4.000ml @10:20:22.56 delay 5 seconds @10:20:27.57 CSR.select :air @10:20:27.66 CS.reconfigure CSfast @10:20:27.70 CS.seek 10.000ml @10:20:42.12 CSV.dial CBV @10:20:42.14
CBV.dial CSV @10:20:43.18 CTV.dial :air @10:20:44.47 CS.seek 0.000ml @10:21:07.74 delay 7 seconds @10:21:14.75 CS.seek 3.000ml @10:21:22.31 CSV.dial :waste @10:21:22.74 CS.seek 0.000ml @10:21:30.31 Flushing upper manifold @10:21:30.40 CSV.dial :flush @10:21:30.83 CS.seek 5.000ml @10:21:42.99 delay 5 seconds @10:21:48.00 CSV.dial :air @10:21:48.48 CS.seek 7.000ml @10:21:53.84 CSV.dial CBV @10:21:53.86
CBV.dial CSV @10:21:54.95 CTV.dial :air @10:21:54.96 CS.seek 0.000ml @10:22:11.62 delay 7 seconds @10:22:18.63 CS.seek 3.000ml @10:22:26.21 CSV.dial :waste @10:22:26.73 CS.seek 0.000ml @10:22:34.56 CSV.dial :flush @10:22:35.00 CS.seek 3.000ml @10:22:42.65 delay 5 seconds @10:22:47.66 CSV.dial :air @10:22:48.16 CS.seek 5.000ml @10:22:53.52 CSV.dial :air @10:22:53.54 CS.seek 0.000ml @10:23:05.61 delay 7 seconds @10:23:12.62 CS.seek 3.000ml @10:23:20.21 CSV.dial :waste @10:23:21.01 CS.seek 0.000ml @10:23:28.55 Flushing lower manifold's waste1 port @10:23:28.65 CSV.dial :flush @10:23:29.15 CS.seek 5.000ml @10:23:41.21 delay 5 seconds @10:23:46.22 CSV.dial :air @10:23:46.71 CS.seek 7.000ml @10:23:51.99 CSV.dial CTV @10:23:52.01
CTV.dial CSV @10:23:53.35 CBV.dial :waste1 @10:23:53.81 CS.seek 0.000ml @10:24:10.27 Flushing lower manifold's waste2b port @10:24:10.36 CSV.dial :flush @10:24:11.15 CS.seek 5.000ml @10:24:23.24 delay 5 seconds @10:24:28.25 CSV.dial :air @10:24:28.80 CS.seek 6.999ml @10:24:34.24 CSV.dial CTV @10:24:34.26
CTV.dial CSV @10:24:34.70 CBV.dial :waste2b @10:24:35.45 CS.seek 0.000ml @10:24:52.23 Disinfecting external Intake with kill @10:24:52.32 CSV.dial CSR @10:24:52.75 CSR.select :kill @10:24:52.85 CS.seek 8.000ml @10:25:11.67 delay 5 seconds @10:25:16.68 CSR.select :air @10:25:16.77 CS.seek 10.000ml @10:25:22.11 CSV.dial CBV @10:25:22.13
CBV.dial CSV @10:25:23.16 CTV.dial :intake @10:25:23.87 Intake.select :open @10:25:23.97 CS.seek 2.000ml @10:25:43.08 Intake.select :close @10:25:43.17 CTV.dial :air @10:25:43.90 CS.reconfigure CSconfig @10:25:43.93 CS.seek 0.000ml @10:25:49.79 CSV.dial CSR @10:25:50.84 CSR.select :flush @10:25:50.94 CS.seek 5.000ml @10:26:04.45 delay 5 seconds @10:26:09.46 CSR.select :air @10:26:09.55 CS.seek 10.000ml @10:26:23.01 CSV.dial CBV @10:26:24.07 CS.seek 0.000ml @10:26:50.23 Garaging FlushPuck in Collection Clamp @10:26:50.29 Elbow.reconfigure TightElbow @10:26:50.32 Elbow.seek CC @10:26:50.42 Elbow.reconfigure LooseElbow @10:26:50.50 Forearm.seek CC @10:26:51.44 CC.seek sealed @10:26:52.39 Hand.grip @10:26:53.07 CC.seek open @10:26:56.39 Forearm.seek retract @10:26:57.33 Elbow.reconfigure TightElbow @10:26:57.37 Elbow.seek garage @10:27:00.17 Elbow.reconfigure LooseElbow @10:27:00.84 Forearm.reconfigure TightForearm @10:27:00.88 Forearm.seek garage @10:27:02.03 Hand.release @10:27:02.86 Forearm.reconfigure LooseForearm @10:27:02.90 Forearm.seek retract @10:27:03.93 Completed {wqPCR} begun 08:01:03 @10:27:03.96 deferred until MFBPCR finish @10:27:03.97
Waiting for MFBPCR til 08:01PST17-Feb-14 @10:27:03.98 Sleeping until 08:01PST17-Feb-14 @10:27:03.99 GoodNews.email "Then, in tube 2: wqPCR\n/home/esp/esp2local/mission/14feb14sccwrp.rb:63",Subject => "Waiting until 08:01PST17-Feb-14" @12:11:42.73 PCR.off @12:11:43.25 PCR for HF183em completed -- closed /var/log/esp/HF183em14feb1610h248ml.pcr @12:11:43.30 Cleaning PCR @12:11:43.31 AFV.select :carrierSolution @12:11:43.40 delay 2 seconds @12:11:45.41 AS.reconfigure ASconfig @12:11:45.44 AS.seek 0.500ml @12:11:55.86 delay 1 second @12:11:56.87 AFV.select :holdingCoil @12:11:56.96 ASV.dial :waste1 @12:11:57.29 ATV.dial :air3 @12:11:58.26 AS.reconfigure ASslow1 @12:11:58.29 AS.seek 0.510ml @12:12:00.50 delay 2 seconds @12:12:02.51 ATV.dial :bleach @12:12:02.90 AS.seek 0.810ml @12:13:04.35 delay 3 seconds @12:13:07.36 ATV.dial :air3 @12:13:07.70 delay 3 seconds @12:13:10.76 AS.seek 0.835ml @12:13:16.08 delay 3 seconds @12:13:19.11 ATV.dial :bleach @12:13:19.50 AS.seek 0.820ml @12:13:22.83 delay 3 seconds @12:13:25.84 ARV.dial :waste @12:13:26.45 delay 3 seconds @12:13:29.46 ATV.dial :ARV @12:13:30.29 AS.reconfigure ASconfig @12:13:30.32 AS.seek 0.470ml @12:13:51.02 AFV.select :carrierSolution @12:13:51.12 delay 2 seconds @12:13:53.13 AS.seek 1.000ml @12:14:24.34 delay 1 second @12:14:25.35 AFV.select :holdingCoil @12:14:25.44 delay 1 second @12:14:26.45 AS.reconfigure ASfast @12:14:26.48 AS.seek 0.000ml @12:14:54.22 AFV.select :carrierSolution @12:14:54.32 delay 2 seconds @12:14:56.33 AS.reconfigure ASconfig @12:14:56.36 AS.seek 1.000ml @12:15:54.98 delay 1 second @12:15:55.99 AFV.select :holdingCoil @12:15:56.08 delay 1 second @12:15:57.68 AS.reconfigure ASfast @12:15:57.71 AS.seek 0.000ml @12:16:25.43 AFV.select :carrierSolution @12:16:25.53 delay 2 seconds @12:16:27.54 AS.reconfigure ASconfig @12:16:27.57 AS.seek 1.000ml @12:17:26.16 delay 1 second @12:17:27.17 AFV.select :holdingCoil @12:17:27.26 delay 1 second @12:17:28.27 AS.reconfigure ASfast @12:17:28.30 AS.seek 0.000ml @12:17:56.03 ARV.dial between carrier and air1 @12:17:56.44 ATV.dial :air3 @12:17:57.38 AS.reconfigure ASslow1 @12:17:57.41 AS.seek 0.010ml @12:17:59.74 delay 2 seconds @12:18:01.75 ATV.dial :bleach @12:18:02.13 AS.seek 0.810ml @12:20:45.54 delay 3 seconds @12:20:48.55 ATV.dial :air3 @12:20:48.97 delay 3 seconds @12:20:52.03 AS.seek 0.835ml @12:20:57.36 delay 3 seconds @12:21:00.37 ATV.dial :bleach @12:21:00.86 AS.seek 0.820ml @12:21:04.18 delay 3 seconds @12:21:07.19 ATV.dial PCR @12:21:07.54 AS.reconfigure ASconfig @12:21:07.57 AS.seek empty @12:21:55.70 PCR.program :bleachProfile @12:21:55.76 PCR program should complete in < 2:45 @12:21:55.77 PCR.run 10:00 @12:24:56.99 PCR.off @12:24:57.02 AFV.select :carrierSolution @12:24:57.12 delay 2 seconds @12:24:59.13 AS.seek 1.000ml @12:25:57.77 delay 1 second @12:25:58.78 AFV.select :holdingCoil @12:25:58.87 delay 1 second @12:25:59.88 AS.reconfigure ASfast @12:25:59.91 AS.seek 0.000ml @12:26:27.64 delay 3 seconds @12:26:30.65 AFV.select :carrierSolution @12:26:30.74 delay 2 seconds @12:26:32.75 AS.reconfigure ASconfig @12:26:32.78 AS.seek 1.000ml @12:27:31.36 delay 1 second @12:27:32.37 AFV.select :holdingCoil @12:27:32.46 delay 1 second @12:27:33.47 AS.reconfigure ASfast @12:27:33.50 AS.seek 0.000ml @12:28:01.23 delay 3 seconds @12:28:04.24 AFV.select :carrierSolution @12:28:04.33 delay 2 seconds @12:28:06.34 AS.reconfigure ASconfig @12:28:06.37 AS.seek 1.000ml @12:29:04.95 delay 1 second @12:29:05.96 AFV.select :holdingCoil @12:29:06.05 delay 1 second @12:29:07.06 AS.reconfigure ASfast @12:29:07.09 AS.seek 0.000ml @12:29:34.78 delay 3 seconds @12:29:37.79 AFV.select :carrierSolution @12:29:37.88 delay 2 seconds @12:29:39.89 AS.reconfigure ASconfig @12:29:39.92 AS.seek 1.000ml @12:30:38.45 delay 1 second @12:30:39.46 AFV.select :holdingCoil @12:30:39.55 delay 1 second @12:30:40.56 AS.reconfigure ASfast @12:30:40.59 AS.seek 0.000ml @12:31:08.29 delay 3 seconds @12:31:11.30 AFV.select :carrierSolution @12:31:11.39 delay 2 seconds @12:31:13.40 AS.reconfigure ASconfig @12:31:13.43 AS.seek 1.000ml @12:32:11.99 delay 1 second @12:32:13.00 AFV.select :holdingCoil @12:32:13.09 delay 1 second @12:32:14.10 AS.reconfigure ASnorm @12:32:14.13 AS.seek 0.000ml @12:33:05.62 Setting up PCR for ENTap -- writing /var/log/esp/ENTap14feb1612h248ml.pcr @12:33:05.63 Building PCR Slug from ASV with MM1 mix, PP1 primer @12:33:05.64 Priming with carrier @12:33:05.65 ATV.dial ARV @12:33:06.16 ARV.dial :carrier @12:33:06.57 AS.seek 0.500ml @12:33:32.48 AFV.select :carrierSolution @12:33:32.59 delay 2 seconds @12:33:34.60 AS.reconfigure ASconfig @12:33:34.63 AS.seek 1.000ml @12:34:04.09 delay 1 second @12:34:05.10 AFV.select :holdingCoil @12:34:05.20 delay 1 second @12:34:06.21 ATV.dial :waste @12:34:07.48 AS.reconfigure ASnorm @12:34:07.51 AS.seek empty @12:34:58.98 AFV.select :carrierSolution @12:34:59.09 delay 2 seconds @12:35:01.10 AS.reconfigure ASconfig @12:35:01.13 AS.seek 0.500ml @12:35:30.60 delay 1 second @12:35:31.61 AFV.select :holdingCoil @12:35:31.71 Building Slug @12:35:31.72 ATV.dial :ARV @12:35:32.98 delay 2 seconds @12:35:34.99 ARV.dial :air1 @12:35:35.31 delay 2 seconds @12:35:37.37 AS.reconfigure ASslow2 @12:35:37.40 AS.seek 0.506ml @12:35:43.92 delay 2 seconds @12:35:45.93 ARV.dial :MM1 @12:35:46.44 delay 2 seconds @12:35:48.44 Adding MM1 @12:35:48.50 AS.seek 0.524ml @12:36:07.38 delay 25 seconds @12:36:32.39 ARV.dial :PP1 @12:36:32.82 Adding PP1 @12:36:32.83 delay 2 seconds @12:36:34.88 AS.seek 0.530ml @12:36:41.23 delay 10 seconds @12:36:51.24 ARV.dial :air1 @12:36:51.58 adding ARV air @12:36:51.59 delay 2 seconds @12:36:53.64 AS.seek 0.536ml @12:37:00.51 delay 5 seconds @12:37:05.52 ARV.dial :carrier @12:37:05.84 delay 7 seconds @12:37:12.90 AS.seek 0.594ml @12:38:12.43 delay 5 seconds @12:38:17.44 ATV.dial :ASV @12:38:17.77 delay 2 seconds @12:38:19.83 AS.seek 0.600ml @12:38:26.30 delay 4 seconds @12:38:30.31 ATV.dial ARV @12:38:30.63 delay 2 seconds @12:38:32.64 ARV.dial :carrier @12:38:32.65 delay 2 seconds @12:38:34.70 AS.seek 0.650ml @12:39:26.00 delay 4 seconds @12:39:30.01 ATV.dial PCR @12:39:30.50 ASV.dial :waste1 @12:39:30.51 ARV.dial :air1 @12:39:30.86 Positioning Slug by 0.324ml @12:39:30.91 AS.seek 0.326ml @12:45:01.57 delay 5 seconds @12:45:06.58 ATV.dial ARV @12:45:07.10 ASV.dial :PCRlock @12:45:07.87 delay 5 seconds @12:45:12.88 Running ENTap Profile60C thermocycling @12:45:12.89 PCR.program Profile60C @12:45:12.99 Profile60C should complete in < 1:39:08 @12:45:13.00 PCR.run 2:08:55 @14:06:47.62 PCR.off @14:06:48.13 PCR for ENTap completed -- closed /var/log/esp/ENTap14feb1612h248ml.pcr @14:06:48.18 Cleaning PCR @14:06:48.19 AFV.select :carrierSolution @14:06:48.28 delay 2 seconds @14:06:50.29 AS.reconfigure ASconfig @14:06:50.32 AS.seek 0.500ml @14:07:00.75 delay 1 second @14:07:01.76 AFV.select :holdingCoil @14:07:01.85 ASV.dial :waste1 @14:07:02.19 ATV.dial :air3 @14:07:03.16 AS.reconfigure ASslow1 @14:07:03.19 AS.seek 0.510ml @14:07:05.47 delay 2 seconds @14:07:07.48 ATV.dial :bleach @14:07:08.44 AS.seek 0.810ml @14:08:09.86 delay 3 seconds @14:08:12.87 ATV.dial :air3 @14:08:13.22 delay 3 seconds @14:08:16.28 AS.seek 0.835ml @14:08:21.59 delay 3 seconds @14:08:24.60 ATV.dial :bleach @14:08:25.00 AS.seek 0.820ml @14:08:28.32 delay 3 seconds @14:08:31.33 ARV.dial :waste @14:08:31.82 delay 3 seconds @14:08:34.83 ATV.dial :ARV @14:08:35.67 AS.reconfigure ASconfig @14:08:35.70 AS.seek 0.470ml @14:08:56.35 AFV.select :carrierSolution @14:08:56.46 delay 2 seconds @14:08:58.47 AS.seek 1.000ml @14:09:29.67 delay 1 second @14:09:30.68 AFV.select :holdingCoil @14:09:30.78 delay 1 second @14:09:31.79 AS.reconfigure ASfast @14:09:31.82 AS.seek 0.000ml @14:09:59.56 AFV.select :carrierSolution @14:09:59.72 delay 2 seconds @14:10:01.73 AS.reconfigure ASconfig @14:10:01.76 AS.seek 1.000ml @14:11:00.35 delay 1 second @14:11:01.36 AFV.select :holdingCoil @14:11:01.45 delay 1 second @14:11:02.46 AS.reconfigure ASfast @14:11:02.49 AS.seek 0.000ml @14:11:30.22 AFV.select :carrierSolution @14:11:30.32 delay 2 seconds @14:11:32.33 AS.reconfigure ASconfig @14:11:32.36 AS.seek 1.000ml @14:12:30.91 delay 1 second @14:12:31.92 AFV.select :holdingCoil @14:12:32.02 delay 1 second @14:12:33.03 AS.reconfigure ASfast @14:12:33.06 AS.seek 0.000ml @14:13:00.78 ARV.dial between carrier and air1 @14:13:01.19 ATV.dial :air3 @14:13:02.12 AS.reconfigure ASslow1 @14:13:02.15 AS.seek 0.010ml @14:13:04.52 delay 2 seconds @14:13:06.53 ATV.dial :bleach @14:13:06.91 AS.seek 0.810ml @14:15:50.35 delay 3 seconds @14:15:53.36 ATV.dial :air3 @14:15:53.70 delay 3 seconds @14:15:56.76 AS.seek 0.835ml @14:16:02.13 delay 3 seconds @14:16:05.14 ATV.dial :bleach @14:16:05.53 AS.seek 0.820ml @14:16:08.85 delay 3 seconds @14:16:11.86 ATV.dial PCR @14:16:12.20 AS.reconfigure ASconfig @14:16:12.23 AS.seek empty @14:17:00.38 PCR.program :bleachProfile @14:17:00.44 PCR program should complete in < 2:45 @14:17:00.45 PCR.run 10:00 @14:20:01.66 PCR.off @14:20:01.69 AFV.select :carrierSolution @14:20:01.78 delay 2 seconds @14:20:03.79 AS.seek 1.000ml @14:21:02.40 delay 1 second @14:21:03.41 AFV.select :holdingCoil @14:21:03.50 delay 1 second @14:21:04.51 AS.reconfigure ASfast @14:21:04.54 AS.seek 0.000ml @14:21:32.23 delay 3 seconds @14:21:35.24 AFV.select :carrierSolution @14:21:35.33 delay 2 seconds @14:21:37.34 AS.reconfigure ASconfig @14:21:37.37 AS.seek 1.000ml @14:22:35.96 delay 1 second @14:22:36.97 AFV.select :holdingCoil @14:22:37.06 delay 1 second @14:22:38.07 AS.reconfigure ASfast @14:22:38.10 AS.seek 0.000ml @14:23:05.79 delay 3 seconds @14:23:08.80 AFV.select :carrierSolution @14:23:08.89 delay 2 seconds @14:23:10.90 AS.reconfigure ASconfig @14:23:10.93 AS.seek 1.000ml @14:24:09.54 delay 1 second @14:24:10.55 AFV.select :holdingCoil @14:24:10.64 delay 1 second @14:24:11.65 AS.reconfigure ASfast @14:24:11.68 AS.seek 0.000ml @14:24:39.43 delay 3 seconds @14:24:42.44 AFV.select :carrierSolution @14:24:42.53 delay 2 seconds @14:24:44.54 AS.reconfigure ASconfig @14:24:44.57 AS.seek 1.000ml @14:25:43.18 delay 1 second @14:25:44.19 AFV.select :holdingCoil @14:25:44.28 delay 1 second @14:25:45.29 AS.reconfigure ASfast @14:25:45.32 AS.seek 0.000ml @14:26:13.05 delay 3 seconds @14:26:16.06 AFV.select :carrierSolution @14:26:16.15 delay 2 seconds @14:26:18.16 AS.reconfigure ASconfig @14:26:18.19 AS.seek 1.000ml @14:27:16.78 delay 1 second @14:27:17.79 AFV.select :holdingCoil @14:27:17.88 delay 1 second @14:27:18.89 AS.reconfigure ASnorm @14:27:18.92 AS.seek 0.000ml @14:28:10.37 Setting up PCR for ENTem -- writing /var/log/esp/ENTem14feb1614h248ml.pcr @14:28:10.38 Building PCR Slug from ASV with MM2 mix, PP3 primer @14:28:10.39 Priming with carrier @14:28:10.40 ATV.dial ARV @14:28:10.91 ARV.dial :carrier @14:28:11.21 AS.seek 0.500ml @14:28:37.08 AFV.select :carrierSolution @14:28:37.19 delay 2 seconds @14:28:39.20 AS.reconfigure ASconfig @14:28:39.23 AS.seek 1.000ml @14:29:08.64 delay 1 second @14:29:09.65 AFV.select :holdingCoil @14:29:09.75 delay 1 second @14:29:10.76 ATV.dial :waste @14:29:12.04 AS.reconfigure ASnorm @14:29:12.07 AS.seek empty @14:30:03.56 AFV.select :carrierSolution @14:30:04.20 delay 2 seconds @14:30:06.21 AS.reconfigure ASconfig @14:30:06.24 AS.seek 0.500ml @14:30:35.73 delay 1 second @14:30:36.74 AFV.select :holdingCoil @14:30:36.84 Building Slug @14:30:36.85 ATV.dial :ARV @14:30:38.12 delay 2 seconds @14:30:40.13 ARV.dial :air1 @14:30:40.46 delay 2 seconds @14:30:42.52 AS.reconfigure ASslow2 @14:30:42.55 AS.seek 0.506ml @14:30:49.21 delay 2 seconds @14:30:51.22 ARV.dial :MM2 @14:30:52.53 delay 2 seconds @14:30:54.54 Adding MM2 @14:30:54.59 AS.seek 0.524ml @14:31:13.34 delay 25 seconds @14:31:38.35 ARV.dial :PP3 @14:31:38.69 Adding PP3 @14:31:38.70 delay 2 seconds @14:31:40.75 AS.seek 0.530ml @14:31:47.12 delay 10 seconds @14:31:57.13 ARV.dial :air2 @14:31:57.56 adding ARV air @14:31:57.57 delay 2 seconds @14:31:59.63 AS.seek 0.536ml @14:32:06.00 delay 5 seconds @14:32:11.01 ARV.dial :carrier @14:32:12.05 delay 7 seconds @14:32:19.11 AS.seek 0.594ml @14:33:18.36 delay 5 seconds @14:33:23.37 ATV.dial :ASV @14:33:23.78 delay 2 seconds @14:33:25.84 AS.seek 0.600ml @14:33:32.08 delay 4 seconds @14:33:36.09 ATV.dial ARV @14:33:36.42 delay 2 seconds @14:33:38.43 ARV.dial :carrier @14:33:38.44 delay 2 seconds @14:33:40.49 AS.seek 0.650ml @14:34:31.69 delay 4 seconds @14:34:35.70 ATV.dial PCR @14:34:36.29 ASV.dial :waste1 @14:34:36.30 ARV.dial :air1 @14:34:36.65 Positioning Slug by 0.324ml @14:34:36.71 AS.seek 0.326ml @14:40:07.33 delay 5 seconds @14:40:12.34 ATV.dial ARV @14:40:12.86 ASV.dial :PCRlock @14:40:13.35 delay 5 seconds @14:40:18.36 Running ENTem ProfileEM60C thermocycling @14:40:18.37 PCR.program ProfileEM60C @14:40:18.48 ProfileEM60C should complete in < 2:11:38 @14:40:18.49 PCR.run 2:49:32.5 @16:35:17.59 PCR.off @16:35:18.13 PCR for ENTem completed -- closed /var/log/esp/ENTem14feb1614h248ml.pcr @16:35:18.18 Cleaning PCR AFV.select :carrierSolution @16:35:18.27 delay 2 seconds @16:35:20.28 AS.reconfigure ASconfig @16:35:20.31 AS.seek 0.500ml @16:35:30.77 delay 1 second @16:35:31.78 AFV.select :holdingCoil @16:35:31.87 ASV.dial :waste1 @16:35:32.63 ATV.dial :air3 @16:35:33.57 AS.reconfigure ASslow1 @16:35:33.60 AS.seek 0.510ml @16:35:35.90 delay 2 seconds @16:35:37.91 ATV.dial :bleach @16:35:38.40 AS.seek 0.810ml @16:36:39.82 delay 3 seconds @16:36:42.83 ATV.dial :air3 @16:36:43.16 delay 3 seconds @16:36:46.22 AS.seek 0.835ml @16:36:51.55 delay 3 seconds @16:36:54.57 ATV.dial :bleach @16:36:54.96 AS.seek 0.820ml @16:36:58.27 delay 3 seconds @16:37:01.28 ARV.dial :waste @16:37:01.88 delay 3 seconds @16:37:04.89 ATV.dial :ARV @16:37:05.63 AS.reconfigure ASconfig @16:37:05.66 AS.seek 0.470ml @16:37:26.35 AFV.select :carrierSolution @16:37:26.44 delay 2 seconds @16:37:28.45 AS.seek 1.000ml @16:37:59.69 delay 1 second @16:38:00.70 AFV.select :holdingCoil @16:38:00.79 delay 1 second @16:38:01.80 AS.reconfigure ASfast @16:38:01.83 AS.seek 0.000ml @16:38:29.55 AFV.select :carrierSolution @16:38:29.65 delay 2 seconds @16:38:31.66 AS.reconfigure ASconfig @16:38:31.69 AS.seek 1.000ml @16:39:30.31 delay 1 second @16:39:31.32 AFV.select :holdingCoil @16:39:31.41 delay 1 second @16:39:32.42 AS.reconfigure ASfast @16:39:32.45 AS.seek 0.000ml @16:40:00.19 AFV.select :carrierSolution @16:40:00.29 delay 2 seconds @16:40:02.30 AS.reconfigure ASconfig @16:40:02.33 AS.seek 1.000ml @16:41:00.89 delay 1 second @16:41:01.90 AFV.select :holdingCoil @16:41:02.11 delay 1 second @16:41:03.12 AS.reconfigure ASfast @16:41:03.15 AS.seek 0.000ml @16:41:30.87 ARV.dial between carrier and air1 @16:41:31.28 ATV.dial :air3 @16:41:32.23 AS.reconfigure ASslow1 @16:41:32.26 AS.seek 0.010ml @16:41:34.60 delay 2 seconds @16:41:36.61 ATV.dial :bleach @16:41:37.00 AS.seek 0.810ml @16:44:20.40 delay 3 seconds @16:44:23.41 ATV.dial :air3 @16:44:23.76 delay 3 seconds @16:44:26.82 AS.seek 0.835ml @16:44:32.18 delay 3 seconds @16:44:35.19 ATV.dial :bleach @16:44:35.68 AS.seek 0.820ml @16:44:39.02 delay 3 seconds @16:44:42.03 ATV.dial PCR @16:44:42.48 AS.reconfigure ASconfig @16:44:42.51 AS.seek empty @16:45:30.65 PCR.program :bleachProfile @16:45:30.70 PCR program should complete in < 2:45 @16:45:30.71 PCR.run 10:00 @16:48:31.93 PCR.off @16:48:31.96 AFV.select :carrierSolution @16:48:32.05 delay 2 seconds @16:48:34.06 AS.seek 1.000ml @16:49:32.68 delay 1 second @16:49:33.69 AFV.select :holdingCoil @16:49:33.78 delay 1 second @16:49:34.79 AS.reconfigure ASfast @16:49:34.82 AS.seek 0.000ml @16:50:02.54 delay 3 seconds @16:50:05.55 AFV.select :carrierSolution @16:50:05.64 delay 2 seconds @16:50:07.65 AS.reconfigure ASconfig @16:50:07.68 AS.seek 1.000ml @16:51:06.28 delay 1 second @16:51:07.29 AFV.select :holdingCoil @16:51:07.38 delay 1 second @16:51:08.39 AS.reconfigure ASfast @16:51:08.42 AS.seek 0.000ml @16:51:36.14 delay 3 seconds @16:51:39.15 AFV.select :carrierSolution @16:51:39.24 delay 2 seconds @16:51:41.25 AS.reconfigure ASconfig @16:51:41.28 AS.seek 1.000ml @16:52:40.48 delay 1 second @16:52:41.49 AFV.select :holdingCoil @16:52:41.58 delay 1 second @16:52:42.59 AS.reconfigure ASfast @16:52:42.62 AS.seek 0.000ml @16:53:10.36 delay 3 seconds @16:53:13.37 AFV.select :carrierSolution @16:53:13.46 delay 2 seconds @16:53:15.47 AS.reconfigure ASconfig @16:53:15.50 AS.seek 1.000ml @16:54:14.09 delay 1 second @16:54:15.10 AFV.select :holdingCoil @16:54:15.19 delay 1 second @16:54:16.20 AS.reconfigure ASfast @16:54:16.23 AS.seek 0.000ml @16:54:43.95 delay 3 seconds @16:54:46.96 AFV.select :carrierSolution @16:54:47.05 delay 2 seconds @16:54:49.06 AS.reconfigure ASconfig @16:54:49.09 AS.seek 1.000ml @16:55:47.70 delay 1 second @16:55:48.71 AFV.select :holdingCoil @16:55:48.80 delay 1 second @16:55:49.81 AS.reconfigure ASnorm @16:55:49.84 AS.seek 0.000ml @16:56:41.30 Setting up PCR for IPCap -- writing /var/log/esp/IPCap14feb1616h248ml.pcr @16:56:41.31 Building PCR Slug from ASV with MM1 mix, PP2 primer @16:56:41.32 Priming with carrier ATV.dial ARV @16:56:42.04 ARV.dial :carrier @16:56:42.32 AS.seek 0.500ml @16:57:08.22 AFV.select :carrierSolution @16:57:08.33 delay 2 seconds @16:57:10.34 AS.reconfigure ASconfig @16:57:10.37 AS.seek 1.000ml @16:57:39.85 delay 1 second @16:57:40.86 AFV.select :holdingCoil @16:57:40.96 delay 1 second @16:57:41.97 ATV.dial :waste @16:57:43.28 AS.reconfigure ASnorm @16:57:43.31 AS.seek empty @16:58:34.80 AFV.select :carrierSolution @16:58:34.91 delay 2 seconds @16:58:36.92 AS.reconfigure ASconfig @16:58:36.95 AS.seek 0.500ml @16:59:06.41 delay 1 second @16:59:07.42 AFV.select :holdingCoil @16:59:07.52 Building Slug @16:59:07.53 ATV.dial :ARV @16:59:08.78 delay 2 seconds @16:59:10.79 ARV.dial :air1 @16:59:11.11 delay 2 seconds @16:59:13.17 AS.reconfigure ASslow2 @16:59:13.20 AS.seek 0.506ml @16:59:19.52 delay 2 seconds @16:59:21.53 ARV.dial :MM1 @16:59:22.04 delay 2 seconds @16:59:24.04 Adding MM1 @16:59:24.09 AS.seek 0.524ml @16:59:42.61 delay 25 seconds @17:00:07.62 ARV.dial :PP2 @17:00:07.94 Adding PP2 @17:00:07.96 delay 2 seconds @17:00:10.01 AS.seek 0.530ml @17:00:16.22 delay 10 seconds @17:00:26.23 ARV.dial :air2 @17:00:26.64 adding ARV air @17:00:26.65 delay 2 seconds @17:00:28.71 AS.seek 0.536ml @17:00:34.95 delay 5 seconds @17:00:39.96 ARV.dial :carrier @17:00:40.98 delay 7 seconds @17:00:48.04 AS.seek 0.594ml @17:01:47.40 delay 5 seconds @17:01:52.41 ATV.dial :ASV @17:01:52.72 delay 2 seconds @17:01:54.78 AS.seek 0.600ml @17:02:01.18 delay 4 seconds @17:02:05.19 ATV.dial ARV @17:02:05.51 delay 2 seconds @17:02:07.52 ARV.dial :carrier @17:02:07.53 delay 2 seconds @17:02:09.58 AS.seek 0.650ml @17:03:00.87 delay 4 seconds @17:03:04.88 ATV.dial PCR @17:03:05.39 ASV.dial :waste1 @17:03:05.40 ARV.dial :air1 @17:03:05.74 Positioning Slug by 0.324ml @17:03:05.80 AS.seek 0.326ml @17:08:36.23 delay 5 seconds @17:08:41.24 ATV.dial ARV @17:08:41.76 ASV.dial :PCRlock @17:08:42.23 delay 5 seconds @17:08:47.24 Running IPCap Profile59C thermocycling @17:08:47.25 PCR.program Profile59C @17:08:47.35 Profile59C should complete in < 1:39:08 @17:08:47.36 PCR.run 2:08:55 @18:31:53.49 PCR.off @18:31:54.02 PCR for IPCap completed -- closed /var/log/esp/IPCap14feb1616h248ml.pcr @18:31:54.07 Cleaning PCR @18:31:54.08 AFV.select :carrierSolution @18:31:54.17 delay 2 seconds @18:31:56.18 AS.reconfigure ASconfig @18:31:56.21 AS.seek 0.500ml @18:32:06.66 delay 1 second @18:32:07.67 AFV.select :holdingCoil @18:32:07.76 ASV.dial :waste1 @18:32:08.18 ATV.dial :air3 @18:32:09.12 AS.reconfigure ASslow1 @18:32:09.15 AS.seek 0.510ml @18:32:11.44 delay 2 seconds @18:32:13.45 ATV.dial :bleach @18:32:13.93 AS.seek 0.810ml @18:33:15.38 delay 3 seconds @18:33:18.39 ATV.dial :air3 @18:33:18.72 delay 3 seconds @18:33:21.78 AS.seek 0.835ml @18:33:27.11 delay 3 seconds @18:33:30.12 ATV.dial :bleach @18:33:30.51 AS.seek 0.820ml @18:33:33.82 delay 3 seconds @18:33:36.83 ARV.dial :waste @18:33:37.33 delay 3 seconds @18:33:40.34 ATV.dial :ARV @18:33:41.07 AS.reconfigure ASconfig @18:33:41.10 AS.seek 0.470ml @18:34:01.78 AFV.select :carrierSolution @18:34:01.87 delay 2 seconds @18:34:03.88 AS.seek 1.000ml @18:34:35.10 delay 1 second @18:34:36.11 AFV.select :holdingCoil @18:34:36.20 delay 1 second @18:34:37.21 AS.reconfigure ASfast @18:34:37.24 AS.seek 0.000ml @18:35:04.98 AFV.select :carrierSolution @18:35:05.08 delay 2 seconds @18:35:07.09 AS.reconfigure ASconfig @18:35:07.12 AS.seek 1.000ml @18:36:05.73 delay 1 second @18:36:06.74 AFV.select :holdingCoil @18:36:06.83 delay 1 second @18:36:07.84 AS.reconfigure ASfast @18:36:07.87 AS.seek 0.000ml @18:36:35.61 AFV.select :carrierSolution @18:36:35.71 delay 2 seconds @18:36:37.72 AS.reconfigure ASconfig @18:36:37.75 AS.seek 1.000ml @18:37:36.34 delay 1 second @18:37:37.35 AFV.select :holdingCoil @18:37:37.44 delay 1 second @18:37:38.45 AS.reconfigure ASfast @18:37:38.48 AS.seek 0.000ml @18:38:06.23 ARV.dial between carrier and air1 @18:38:06.65 ATV.dial :air3 @18:38:07.59 AS.reconfigure ASslow1 @18:38:07.62 AS.seek 0.010ml @18:38:09.93 delay 2 seconds @18:38:11.94 ATV.dial :bleach @18:38:12.34 AS.seek 0.810ml @18:40:55.75 delay 3 seconds @18:40:58.76 ATV.dial :air3 @18:40:59.10 delay 3 seconds @18:41:02.16 AS.seek 0.835ml @18:41:07.50 delay 3 seconds @18:41:10.51 ATV.dial :bleach @18:41:10.99 AS.seek 0.820ml @18:41:14.33 delay 3 seconds @18:41:17.34 ATV.dial PCR @18:41:18.37 AS.reconfigure ASconfig @18:41:18.40 AS.seek empty @18:42:06.52 PCR.program :bleachProfile @18:42:06.58 PCR program should complete in < 2:45 @18:42:06.59 PCR.run 10:00 @18:45:07.80 PCR.off @18:45:07.83 AFV.select :carrierSolution @18:45:07.92 delay 2 seconds @18:45:09.93 AS.seek 1.000ml @18:46:08.53 delay 1 second @18:46:09.54 AFV.select :holdingCoil @18:46:09.63 delay 1 second @18:46:10.64 AS.reconfigure ASfast @18:46:10.67 AS.seek 0.000ml @18:46:38.40 delay 3 seconds @18:46:41.41 AFV.select :carrierSolution @18:46:41.50 delay 2 seconds @18:46:43.51 AS.reconfigure ASconfig @18:46:43.54 AS.seek 1.000ml @18:47:42.15 delay 1 second @18:47:43.16 AFV.select :holdingCoil @18:47:43.25 delay 1 second @18:47:44.26 AS.reconfigure ASfast @18:47:44.29 AS.seek 0.000ml @18:48:12.03 delay 3 seconds @18:48:15.04 AFV.select :carrierSolution @18:48:15.13 delay 2 seconds @18:48:17.14 AS.reconfigure ASconfig @18:48:17.17 AS.seek 1.000ml @18:49:15.76 delay 1 second @18:49:16.77 AFV.select :holdingCoil @18:49:16.86 delay 1 second @18:49:17.87 AS.reconfigure ASfast @18:49:17.90 AS.seek 0.000ml @18:49:45.65 delay 3 seconds @18:49:48.66 AFV.select :carrierSolution @18:49:48.75 delay 2 seconds @18:49:50.76 AS.reconfigure ASconfig @18:49:50.79 AS.seek 1.000ml @18:50:49.36 delay 1 second @18:50:50.37 AFV.select :holdingCoil @18:50:50.46 delay 1 second @18:50:51.47 AS.reconfigure ASfast @18:50:51.50 AS.seek 0.000ml @18:51:19.24 delay 3 seconds @18:51:22.25 AFV.select :carrierSolution @18:51:22.34 delay 2 seconds @18:51:24.35 AS.reconfigure ASconfig @18:51:24.38 AS.seek 1.000ml @18:52:22.95 delay 1 second @18:52:23.96 AFV.select :holdingCoil @18:52:24.05 delay 1 second @18:52:25.06 AS.reconfigure ASnorm @18:52:25.09 AS.seek 0.000ml @18:53:16.54 Setting up PCR for IPCem -- writing /var/log/esp/IPCem14feb1618h248ml.pcr @18:53:16.55 Building PCR Slug from ASV with MM3 mix, PP5 primer @18:53:16.56 Priming with carrier @18:53:16.57 ATV.dial ARV @18:53:17.18 ARV.dial :carrier @18:53:17.46 AS.seek 0.500ml @18:53:43.37 AFV.select :carrierSolution @18:53:43.48 delay 2 seconds @18:53:45.49 AS.reconfigure ASconfig @18:53:45.52 AS.seek 1.000ml @18:54:14.97 delay 1 second @18:54:15.98 AFV.select :holdingCoil @18:54:16.08 delay 1 second @18:54:17.09 ATV.dial :waste @18:54:18.36 AS.reconfigure ASnorm @18:54:18.39 AS.seek empty @18:55:09.86 AFV.select :carrierSolution @18:55:09.97 delay 2 seconds @18:55:11.98 AS.reconfigure ASconfig @18:55:12.13 AS.seek 0.500ml @18:55:41.61 delay 1 second @18:55:42.62 AFV.select :holdingCoil @18:55:42.72 Building Slug @18:55:42.73 ATV.dial :ARV @18:55:43.99 delay 2 seconds @18:55:46.00 ARV.dial :air1 @18:55:46.33 delay 2 seconds @18:55:48.39 AS.reconfigure ASslow2 @18:55:48.42 AS.seek 0.506ml @18:55:54.75 delay 2 seconds @18:55:56.76 ARV.dial :MM3 @18:55:57.61 delay 2 seconds @18:55:59.62 Adding MM3 @18:55:59.67 AS.seek 0.524ml @18:56:18.33 delay 25 seconds @18:56:43.34 ARV.dial :PP5 @18:56:43.67 Adding PP5 @18:56:43.68 delay 2 seconds @18:56:45.74 AS.seek 0.530ml @18:56:52.16 delay 10 seconds @18:57:02.17 ARV.dial :air3 @18:57:02.48 adding ARV air @18:57:02.49 delay 2 seconds @18:57:04.55 AS.seek 0.536ml @18:57:11.11 delay 5 seconds @18:57:16.12 ARV.dial :carrier @18:57:17.16 delay 7 seconds @18:57:24.22 AS.seek 0.594ml @18:58:23.67 delay 5 seconds @18:58:28.68 ATV.dial :ASV @18:58:29.11 delay 2 seconds @18:58:31.17 AS.seek 0.600ml @18:58:37.51 delay 4 seconds @18:58:41.52 ATV.dial ARV @18:58:41.82 delay 2 seconds @18:58:43.83 ARV.dial :carrier @18:58:43.84 delay 2 seconds @18:58:45.89 AS.seek 0.650ml @18:59:37.03 delay 4 seconds @18:59:41.04 ATV.dial PCR @18:59:41.65 ASV.dial :waste1 @18:59:41.66 ARV.dial :air1 @18:59:42.00 Positioning Slug by 0.324ml @18:59:42.05 AS.seek 0.326ml @19:05:12.76 delay 5 seconds @19:05:17.77 ATV.dial ARV @19:05:18.28 ASV.dial :PCRlock @19:05:19.31 delay 5 seconds @19:05:24.32 Running IPCem ProfileEM59C thermocycling @19:05:24.33 PCR.program ProfileEM59C @19:05:24.44 ProfileEM59C should complete in < 2:11:38 @19:05:24.45 PCR.run 2:49:32.5 @21:00:56.35 PCR.off @21:00:56.89 PCR for IPCem completed -- closed /var/log/esp/IPCem14feb1618h248ml.pcr @21:00:56.90 Full MFB Decontamination @21:00:56.91 Decon extraction column and decon AIV @21:00:56.92 SPE.hold 2:46:40,55.0C @21:00:56.94 ASV.dial :SPEdelivery @21:00:57.63 delay 20 seconds @21:01:17.64 AFV.select :carrierSolution @21:01:17.73 delay 2 seconds @21:01:19.74 AS.reconfigure ASconfig @21:01:19.77 AS.seek 0.500ml @21:01:30.21 delay 1 second @21:01:31.22 AFV.select :holdingCoil @21:01:31.31 ATV.dial :air1 @21:01:32.84 AS.reconfigure ASslow1 @21:01:32.87 AS.seek 0.520ml @21:01:37.34 ATV.dial :ASV @21:01:38.61 ASV.dial :SPErecovery @21:01:39.16 AS.seek 0.620ml @21:01:59.80 ATV.dial :waste @21:02:01.25 AS.reconfigure ASnorm @21:02:01.28 AS.seek empty @21:02:33.33 AFV.select :holdingCoil @21:02:33.36 ATV.dial :waste @21:02:33.37 AS.reconfigure ASconfig @21:02:33.40 AS.seek 0.000ml @21:02:33.69 AFV.select :carrierSolution @21:02:33.79 delay 2 seconds @21:02:35.80 AS.seek 0.500ml @21:03:05.24 delay 1 second @21:03:06.25 AFV.select :holdingCoil @21:03:06.34 ATV.dial :air3 @21:03:07.47 AS.reconfigure ASslow1 @21:03:07.50 AS.seek 0.520ml @21:03:11.83 ATV.dial :air3 @21:03:11.89 AS.seek 0.530ml @21:03:14.20 delay 2 seconds @21:03:16.21 ATV.dial :bleach @21:03:16.58 AS.seek 0.710ml @21:03:53.53 delay 3 seconds @21:03:56.54 ATV.dial :air3 @21:03:56.88 delay 3 seconds @21:03:59.94 AS.seek 0.735ml @21:04:05.30 delay 3 seconds @21:04:08.31 ATV.dial :bleach @21:04:08.71 AS.seek 0.720ml @21:04:12.02 delay 3 seconds @21:04:15.04 ATV.dial ASV @21:04:15.92 ASV.dial :SPErecovery @21:04:15.98 AS.seek 0.365ml @21:05:28.62 ATV.dial :waste @21:05:30.08 AS.reconfigure ASnorm @21:05:30.11 AS.seek empty @21:05:49.13 ATV.dial :air3 @21:05:49.70 AS.reconfigure ASslow1 @21:05:49.73 AS.seek 0.009ml @21:05:52.07 delay 2 seconds @21:05:54.08 ATV.dial :bleach @21:05:54.46 AS.seek 0.579ml @21:07:50.97 delay 3 seconds @21:07:53.98 ATV.dial :air3 @21:07:54.32 delay 3 seconds @21:07:57.38 AS.seek 0.604ml @21:08:02.72 delay 3 seconds @21:08:05.73 ATV.dial :bleach @21:08:06.12 AS.seek 0.589ml @21:08:09.46 delay 3 seconds @21:08:12.47 ATV.dial :ASV @21:08:13.46 ASV.dial :wasteR @21:08:13.97 AS.seek 0.000ml @21:10:14.37 delay 1:00 @21:11:14.38 ATV.dial ASV @21:11:14.39 AFV.select :carrierSolution @21:11:14.48 delay 2 seconds @21:11:16.49 AS.reconfigure ASconfig @21:11:16.52 AS.seek 1.000ml @21:12:15.07 delay 1 second @21:12:16.08 AFV.select :holdingCoil @21:12:16.17 delay 1 second @21:12:17.18 ASV.dial :wasteR @21:12:17.19 AS.reconfigure ASslow1 @21:12:17.22 AS.seek 0.000ml @21:15:41.49 delay 10 seconds @21:15:51.50 ATV.dial :air3 @21:15:52.62 AS.seek 0.010ml @21:15:54.96 delay 2 seconds @21:15:56.97 ATV.dial :bleach @21:15:57.35 AS.seek 0.580ml @21:17:53.83 delay 3 seconds @21:17:56.84 ATV.dial :air3 @21:17:57.20 delay 3 seconds @21:18:00.25 AS.seek 0.605ml @21:18:05.62 delay 3 seconds @21:18:08.63 ATV.dial :bleach @21:18:09.02 AS.seek 0.590ml @21:18:12.33 delay 3 seconds @21:18:15.34 ATV.dial ASV @21:18:16.22 ASV.dial :SPEdelivery @21:18:16.54 AS.seek 0.000ml @21:20:17.12 delay 1:00 @21:21:17.14 AFV.select :carrierSolution @21:21:17.24 delay 2 seconds @21:21:19.25 AS.reconfigure ASconfig @21:21:19.28 AS.seek 1.000ml @21:22:17.83 delay 1 second @21:22:18.84 AFV.select :holdingCoil @21:22:18.94 delay 1 second @21:22:19.95 ATV.dial ASV @21:22:19.96 ASV.dial :SPEdelivery @21:22:19.97 AS.reconfigure ASslow1 @21:22:20.00 AS.seek 0.000ml @21:25:44.24 delay 10 seconds @21:25:54.25 ATV.dial :air2 @21:25:54.97 AS.reconfigure ASslow0 @21:25:55.00 AS.seek 0.800ml @21:27:16.93 delay 3 seconds @21:27:19.94 ATV.dial :ASV @21:27:20.66 ASV.dial :SPEdelivery @21:27:20.68 AS.reconfigure ASslow1 @21:27:20.71 AS.seek 0.030ml @21:29:58.00 delay 20 seconds @21:30:18.01 ATV.dial :waste,via => air3 @21:30:19.46 AS.reconfigure ASconfig @21:30:19.49 AS.seek empty @21:30:21.58 SPE.coast @21:30:21.66 Removing Bleach from Mixing Coil @21:30:21.67 AFV.select :carrierSolution @21:30:21.76 delay 2 seconds @21:30:23.77 AS.seek 0.500ml @21:30:53.25 delay 1 second @21:30:54.26 AFV.select :holdingCoil @21:30:54.35 ATV.dial :ASV @21:30:55.80 ASV.dial :SPErecovery @21:30:56.50 AS.reconfigure ASslow1 @21:30:56.53 AS.seek 0.730ml @21:31:43.70 ASV.dial :wasteD @21:31:44.21 AS.reconfigure ASnorm @21:31:44.24 AS.seek 0.000ml @21:32:21.89 AFV.select :carrierSolution @21:32:21.98 delay 2 seconds @21:32:23.99 AS.reconfigure ASconfig @21:32:24.02 AS.seek 0.500ml @21:32:53.48 delay 1 second @21:32:54.49 AFV.select :holdingCoil @21:32:54.58 ATV.dial ASV @21:32:54.59 ASV.dial :SPErecovery @21:32:55.30 AS.reconfigure ASslow0 @21:32:55.33 AS.seek 0.300ml @21:33:16.03 ATV.dial :air2 @21:33:16.80 AS.reconfigure ASslow1 @21:33:16.83 AS.seek 0.320ml @21:33:21.17 ATV.dial ASV @21:33:22.58 AS.reconfigure ASslow0 @21:33:22.61 AS.seek 0.570ml @21:33:48.43 ASV.dial :wasteD @21:33:49.36 AS.reconfigure ASnorm @21:33:49.39 AS.seek 0.000ml @21:34:18.85 AFV.select :carrierSolution @21:34:18.96 delay 2 seconds @21:34:20.97 AS.reconfigure ASconfig @21:34:21.00 AS.seek 0.500ml @21:34:50.46 delay 1 second @21:34:51.47 AFV.select :holdingCoil @21:34:51.56 ATV.dial ASV @21:34:51.57 ASV.dial :SPErecovery @21:34:52.12 AS.reconfigure ASslow0 @21:34:52.15 AS.seek 0.300ml @21:35:12.85 ATV.dial :air2 @21:35:13.68 AS.reconfigure ASslow1 @21:35:13.71 AS.seek 0.320ml @21:35:18.09 ATV.dial ASV @21:35:18.85 AS.reconfigure ASslow0 @21:35:18.88 AS.seek 0.570ml @21:35:44.71 ASV.dial :wasteD @21:35:45.38 AS.reconfigure ASnorm @21:35:45.41 AS.seek 0.000ml @21:36:14.87 ATV.dial :air2 @21:36:15.59 AS.reconfigure ASslow0 @21:36:15.62 AS.seek 0.300ml @21:36:46.54 ATV.dial :ASV @21:36:47.24 ASV.dial :wasteD @21:36:47.26 AS.reconfigure ASconfig @21:36:47.29 AS.seek 0.050ml @21:37:02.15 ATV.dial :waste @21:37:03.62 AS.seek 0.000ml @21:37:06.79 ASV.dial :SPEdelivery @21:37:08.07 deconLysateINT @21:37:08.08 ATV.dial :air3 @21:37:08.65 AS.reconfigure ASslow1 @21:37:08.68 AS.seek 0.010ml @21:37:11.04 delay 2 seconds @21:37:13.05 ATV.dial :bleach @21:37:13.43 AS.seek 0.960ml @21:40:27.54 delay 3 seconds @21:40:30.55 ATV.dial :air3 @21:40:30.90 delay 3 seconds @21:40:33.96 AS.seek 0.985ml @21:40:39.37 delay 3 seconds @21:40:42.38 ATV.dial :bleach @21:40:42.87 AS.seek 0.970ml @21:40:46.27 delay 3 seconds @21:40:49.28 ATV.dial :AIV @21:40:50.46 AIV.dial :coilToMFB @21:40:50.47 AWV.select :waste @21:40:50.48 AFV.select :carrierSolution @21:40:50.57 delay 2 seconds @21:40:52.58 AS.reconfigure ASconfig @21:40:52.61 AS.seek 1.000ml @21:40:54.68 delay 1 second @21:40:55.69 AFV.select :holdingCoil @21:40:55.79 delay 1 second @21:40:56.80 AS.reconfigure ASslow0 @21:40:56.83 AS.seek empty @21:42:39.38 AFV.select :carrierSolution @21:42:39.49 delay 2 seconds @21:42:41.50 AS.reconfigure ASconfig @21:42:41.53 AS.seek 1.000ml @21:43:40.17 delay 1 second @21:43:41.18 AFV.select :holdingCoil @21:43:41.28 delay 1 second @21:43:42.29 AS.reconfigure ASslow0 @21:43:42.32 AS.seek empty @21:45:24.73 AFV.select :carrierSolution @21:45:24.84 delay 2 seconds @21:45:26.85 AS.reconfigure ASconfig @21:45:26.88 AS.seek 1.000ml @21:46:25.54 delay 1 second @21:46:26.55 AFV.select :holdingCoil @21:46:26.65 delay 1 second @21:46:27.66 AS.reconfigure ASslow0 @21:46:27.69 AS.seek empty @21:48:10.10 ATV.dial :air2 @21:48:10.68 AS.reconfigure ASslow1 @21:48:10.71 AS.seek 0.009ml @21:48:13.06 AS.reconfigure ASslow0 @21:48:13.10 AS.seek 1.000ml @21:49:54.41 ATV.dial AIV @21:49:54.94 AS.seek 0.020ml @21:51:35.25 ATV.dial :waste @21:51:36.53 AS.seek 0.000ml @21:51:38.85 AWV.select :air @21:51:38.99 Cleaning PCR @21:51:39.00 AFV.select :carrierSolution @21:51:39.09 delay 2 seconds @21:51:41.10 AS.reconfigure ASconfig @21:51:41.13 AS.seek 0.500ml @21:52:10.53 delay 1 second @21:52:11.54 AFV.select :holdingCoil @21:52:11.63 ASV.dial :waste1 @21:52:12.11 ATV.dial :air3 @21:52:12.68 AS.reconfigure ASslow1 @21:52:12.71 AS.seek 0.510ml @21:52:15.03 delay 2 seconds @21:52:17.04 ATV.dial :bleach @21:52:17.41 AS.seek 0.810ml @21:53:18.89 delay 3 seconds @21:53:21.90 ATV.dial :air3 @21:53:22.33 delay 3 seconds @21:53:25.39 AS.seek 0.835ml @21:53:30.73 delay 3 seconds @21:53:33.74 ATV.dial :bleach @21:53:34.11 AS.seek 0.820ml @21:53:37.42 delay 3 seconds @21:53:40.43 ARV.dial :waste @21:53:41.05 delay 3 seconds @21:53:44.06 ATV.dial :ARV @21:53:44.81 AS.reconfigure ASconfig @21:53:44.84 AS.seek 0.470ml @21:54:05.53 AFV.select :carrierSolution @21:54:05.62 delay 2 seconds @21:54:07.63 AS.seek 1.000ml @21:54:38.82 delay 1 second @21:54:39.83 AFV.select :holdingCoil @21:54:40.58 delay 1 second @21:54:41.59 AS.reconfigure ASfast @21:54:41.62 AS.seek 0.000ml @21:55:09.35 AFV.select :carrierSolution @21:55:09.45 delay 2 seconds @21:55:11.46 AS.reconfigure ASconfig @21:55:11.49 AS.seek 1.000ml @21:56:10.13 delay 1 second @21:56:11.14 AFV.select :holdingCoil @21:56:11.23 delay 1 second @21:56:12.24 AS.reconfigure ASfast @21:56:12.28 AS.seek 0.000ml @21:56:40.03 AFV.select :carrierSolution @21:56:40.13 delay 2 seconds @21:56:42.14 AS.reconfigure ASconfig @21:56:42.17 AS.seek 1.000ml @21:57:40.78 delay 1 second @21:57:41.79 AFV.select :holdingCoil @21:57:41.88 delay 1 second @21:57:42.89 AS.reconfigure ASfast @21:57:42.92 AS.seek 0.000ml @21:58:10.62 ARV.dial between carrier and air1 @21:58:11.11 ATV.dial :air3 @21:58:12.05 AS.reconfigure ASslow1 @21:58:12.08 AS.seek 0.010ml @21:58:14.42 delay 2 seconds @21:58:16.43 ATV.dial :bleach @21:58:16.91 AS.seek 0.810ml @22:01:00.31 delay 3 seconds @22:01:03.32 ATV.dial :air3 @22:01:03.65 delay 3 seconds @22:01:06.71 AS.seek 0.835ml @22:01:12.06 delay 3 seconds @22:01:15.07 ATV.dial :bleach @22:01:15.47 AS.seek 0.820ml @22:01:18.81 delay 3 seconds @22:01:21.82 ATV.dial PCR @22:01:22.14 AS.reconfigure ASconfig @22:01:22.17 AS.seek empty @22:02:10.29 PCR.program :bleachProfile @22:02:10.35 PCR program should complete in < 2:45 @22:02:10.36 PCR.run 10:00 @22:05:14.59 PCR.off @22:05:14.62 AFV.select :carrierSolution @22:05:14.71 delay 2 seconds @22:05:16.72 AS.seek 1.000ml @22:06:15.31 delay 1 second @22:06:16.32 AFV.select :holdingCoil @22:06:16.41 delay 1 second @22:06:17.42 AS.reconfigure ASfast @22:06:17.45 AS.seek 0.000ml @22:06:45.16 delay 3 seconds @22:06:48.17 AFV.select :carrierSolution @22:06:48.26 delay 2 seconds @22:06:50.27 AS.reconfigure ASconfig @22:06:50.30 AS.seek 1.000ml @22:07:48.87 delay 1 second @22:07:49.88 AFV.select :holdingCoil @22:07:49.97 delay 1 second @22:07:50.98 AS.reconfigure ASfast @22:07:51.01 AS.seek 0.000ml @22:08:18.71 delay 3 seconds @22:08:21.72 AFV.select :carrierSolution @22:08:21.81 delay 2 seconds @22:08:23.82 AS.reconfigure ASconfig @22:08:23.85 AS.seek 1.000ml @22:09:22.46 delay 1 second @22:09:23.47 AFV.select :holdingCoil @22:09:23.56 delay 1 second @22:09:24.57 AS.reconfigure ASfast @22:09:24.60 AS.seek 0.000ml @22:09:52.31 delay 3 seconds @22:09:55.32 AFV.select :carrierSolution @22:09:55.41 delay 2 seconds @22:09:57.42 AS.reconfigure ASconfig @22:09:57.45 AS.seek 1.000ml @22:10:56.07 delay 1 second @22:10:57.08 AFV.select :holdingCoil @22:10:57.30 delay 1 second @22:10:58.31 AS.reconfigure ASfast @22:10:58.34 AS.seek 0.000ml @22:11:26.08 delay 3 seconds @22:11:29.09 AFV.select :carrierSolution @22:11:29.18 delay 2 seconds @22:11:31.19 AS.reconfigure ASconfig @22:11:31.22 AS.seek 1.000ml @22:12:29.83 delay 1 second @22:12:30.84 AFV.select :holdingCoil @22:12:30.93 delay 1 second @22:12:31.94 AS.reconfigure ASnorm @22:12:31.97 AS.seek 0.000ml @22:13:23.41 AFV.select :carrierSolution @22:13:23.50 delay 2 seconds @22:13:25.51 AS.reconfigure ASconfig @22:13:25.54 AS.seek 1.000ml @22:14:24.15 delay 1 second @22:14:25.16 AFV.select :holdingCoil @22:14:25.25 delay 1 second @22:14:26.26 ATV.dial :waste @22:14:27.23 AS.reconfigure ASnorm @22:14:27.27 AS.seek 0.500ml @22:14:53.21 ATV.dial :waste2 @22:14:54.30 AS.seek 0.000ml @22:15:20.16 AS.stop @22:15:20.18 CC.stop @22:15:20.21 CS.stop @22:15:20.24 PC.stop @22:15:20.26 PS.stop @22:15:20.29 Forearm.stop @22:15:20.31 Elbow.stop @22:15:20.34 SS.stop @22:15:20.41 Sleepy.power :raw,:OFF @22:15:20.42 Sleepy.power :dwarf,:OFF Shutdown @22:15:20.43 GoodNews.email "MFBPCR completed",Subject => "Shutdown" @02:42:11.54PST17-Feb-14 Session Begun @02:42:11.56 -> Sleepy.cycleModemPower! 9 @02:42:11.58 Session Ended @08:01:03.39
Powering up Dwarves @08:01:08.35 Sleepy.power :raw,:ON @08:01:08.81 SPE.configure SPEconfig @08:01:08.83 AS.configure ASconfig @08:01:08.86 CC.configure CCconfig @08:01:08.89 CS.configure CSconfig @08:01:08.92 PC.configure PCconfig @08:01:08.95 PS.configure PSconfig @08:01:08.98 Forearm.configure LooseForearm @08:01:09.01 Elbow.configure LooseElbow @08:01:09.04 SC.configure SCloose @08:01:09.07 SE.configure SEconfig @08:01:09.10 SS.configure SSconfig @08:01:09.13 CH.configure CHconfig @08:01:09.15 PH.configure PHconfig @08:01:09.18 Valve.reset @08:01:09.86 PCR.off @08:01:09.89 PCR.pidtemp= 50,0.1,0,300,25,25 @08:01:09.92 PCR.caltemp= 6.6661,0.0344,0.0 @08:01:10.02 Intake.select :close @08:01:10.05 Exhaust.select :close CTV.dial :air @08:01:10.09 PSV.dial :air @08:01:10.13
PC.reconfigure PCconfig @08:01:10.18 PC.home.seek open @08:01:10.20 CSV.dial :waste1 @08:01:10.60 PS.reconfigure PSconfig @08:01:10.63 PS.home.seek 0.000ml @08:01:10.67 SSV.dial :spit,avoiding => exhaust @08:01:11.48 CS.reconfigure CSfast @08:01:11.51 SS.reconfigure SSconfig @08:01:11.60 SS.home.seek 0.00ml @08:01:11.62 CS.home.seek 0.000ml @08:01:11.69 CC.reconfigure CCconfig @08:01:11.73 CC.home.seek open @08:01:20.97 PS.coast @08:01:21.05
CC.seek open @08:01:21.07 PC.seek open @08:01:21.21
Forearm.reconfigure LooseForearm @08:01:21.24 Forearm.home.seek retract @08:01:22.04 Hand.grip @08:01:22.06
Elbow.jog -1500 @08:01:22.58 Elbow.reconfigure LooseElbow @08:01:22.62 Elbow.home.seek clear @08:01:27.35 SE.reconfigure SEconfig @08:01:27.38 SE.home.seek down @08:01:27.93 SC.reconfigure SCsloppy @08:01:27.97 SC.home.seek initial @08:01:30.17 Forearm.seek retract @08:01:30.23 Elbow.seek hammer @08:01:31.42 Forearm.reconfigure TightForearm @08:01:31.47 Forearm.seek 2 @08:01:32.60 Forearm.seek retract @08:01:33.69 Forearm.reconfigure LooseForearm @08:01:34.22PST17-Feb-14 ===> STARTED wqPCR (phase #4) <=== @08:01:34.23 Starting initialPurge @08:01:34.24 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:01:34.29 Hand.release @08:01:35.11 Elbow.reconfigure TightElbow @08:01:35.14 Elbow.seek FlushPuck::Garage @08:01:36.92 Elbow.reconfigure LooseElbow @08:01:37.00 Forearm.seek garage @08:01:37.95 Hand.grip @08:01:38.74 Forearm.seek retract @08:01:39.76 Elbow.reconfigure TightElbow @08:01:39.80 Elbow.seek CC @08:01:42.59 Elbow.reconfigure LooseElbow @08:01:42.67 Forearm.reconfigure TightForearm @08:01:42.70 Forearm.seek CC @08:01:43.82 CC.seek closed @08:01:47.57 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.95 @08:01:47.64 Hand.release @08:01:48.44 Forearm.reconfigure LooseForearm @08:01:48.47 Forearm.seek retract @08:01:49.44 Flushing the Collection Valve::Manifold @08:01:49.53 CS Pulling 5ml of flush @08:01:49.54 CSV.dial CSR @08:01:51.02 CSR.select :flush @08:01:51.12 CS.reconfigure CSconfig @08:01:51.16 CS.seek 5.000ml @08:02:04.66 delay 5 seconds @08:02:09.67 CSR.select :air @08:02:09.81 CS.reconfigure CSfast @08:02:09.84 CS.seek 7.500ml @08:02:16.53 CSV.dial :waste @08:02:17.80 CS.reconfigure CSconfig @08:02:17.84 CS.seek 0.000ml @08:02:37.66 Purging external Intake @08:02:37.75 CSV.dial :flush @08:02:38.26 CS.reconfigure CSfast @08:02:38.29 CS.seek 10.000ml @08:03:02.00 delay 5 seconds @08:03:07.01 CSV.dial CBV @08:03:07.03
CBV.dial CSV @08:03:07.83 CTV.dial :intake @08:03:08.56 Intake.select :open @08:03:08.65 CS.seek 0.000ml @08:03:33.19 Intake.select :close @08:03:33.28 CTV.dial :air @08:03:34.01 Flushing upper manifold @08:03:34.10 CSV.dial :flush @08:03:34.90 CS.seek 5.000ml @08:03:47.25 delay 5 seconds @08:03:52.26 CSV.dial :air @08:03:52.75 CS.seek 7.000ml @08:03:58.22 CSV.dial CBV @08:03:58.24
CBV.dial CSV @08:03:59.31 CTV.dial :air @08:03:59.33 CS.seek 0.000ml @08:04:16.25 delay 7 seconds @08:04:23.26 CS.seek 3.000ml @08:04:30.96 CSV.dial :waste @08:04:31.48 CS.seek 0.000ml @08:04:39.29 CSV.dial :flush @08:04:39.72 CS.seek 3.000ml @08:04:47.43 delay 5 seconds @08:04:52.45 CSV.dial :air @08:04:52.92 CS.seek 5.000ml @08:04:58.31 CSV.dial :air @08:04:58.32 CS.seek 0.000ml @08:05:10.74 delay 7 seconds @08:05:17.75 CS.seek 3.000ml @08:05:25.59 CSV.dial :waste @08:05:26.38 CS.seek 0.000ml @08:05:34.09 Garaging FlushPuck in Collection Clamp @08:05:34.15 Elbow.reconfigure TightElbow @08:05:34.18 Elbow.seek CC @08:05:34.37 Elbow.reconfigure LooseElbow @08:05:34.46 Forearm.seek CC @08:05:35.40 CC.seek sealed @08:05:36.34 Hand.grip @08:05:37.01 CC.seek open @08:05:40.30 Forearm.seek retract @08:05:41.25 Elbow.reconfigure TightElbow @08:05:41.29 Elbow.seek garage @08:05:44.58 Elbow.reconfigure LooseElbow @08:05:44.66 Forearm.reconfigure TightForearm @08:05:44.69 Forearm.seek garage @08:05:45.86 Hand.release @08:05:46.68 Forearm.reconfigure LooseForearm @08:05:46.71 Forearm.seek retract @08:05:47.82 Starting mfb @08:05:47.83 pcrBAC sampling at most 1000ml, wcr at most 1000ml @08:05:47.85 Loading sh1 puck from tube 2 into Collection Clamp @08:05:47.88 BACfans.email "Can@08:01:34, 13.0C, 64% humidity, 14.0psia, 13.734V, 0.616A, 0.616A avg, 8.46W\nCTD data unavailable\nISUS data unavailable",Subject => "pcrBAC sampling at most 1000ml, wcr at most 1000ml" @08:05:49.76 Readying the MFB @08:05:49.79 Homing MFB Syringe @08:05:49.80 AFV.select :holdingCoil @08:05:50.02
SC.reconfigure SCloose @08:05:50.04 ATV.dial :air2 @08:05:50.26
Elbow.reconfigure TightElbow @08:05:50.29 Elbow.seek 2 @08:05:50.42 AS.reconfigure ASconfig @08:05:50.45 AS.home.seek home @08:05:52.15
Forearm.reconfigure TightForearm @08:05:52.18 Forearm.seek 2 + 800 counts @08:05:53.21 SE.reconfigure SEpick @08:05:53.26 SE.seek beamBreak @08:05:55.34 SE.configure SEconfig @08:05:55.47 Elbow.seek 2 @08:05:55.62 Forearm.seek 2 + 300 counts @08:05:55.85 Forearm.reconfigure LooseForearm @08:05:55.89 Hand.grip @08:05:56.66 SE.seek down @08:05:56.79
Forearm.seek retract @08:05:57.78 Elbow.seek CC @08:05:57.96 AFV.select :carrierSolution @08:05:58.06 delay 2 seconds @08:05:58.54 SE.coast @08:05:59.38
Elbow.reconfigure LooseElbow @08:05:59.46 Forearm.reconfigure TightForearm @08:05:59.49 Forearm.seek CC @08:06:00.07 AS.seek 1.000ml @08:06:00.64
CC.seek closed @08:06:04.43 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.95 @08:06:04.44 Hand.release @08:06:05.18 Forearm.reconfigure LooseForearm @08:06:05.21 Forearm.seek retract @08:06:06.21 SC.coast @08:06:06.24 collectSample 1000ml w/bubble point of 55psi Priming 7ml @08:06:06.25 Sleepy.power :sampler,:ON @08:06:06.26 Intake.select :close @08:06:06.27 Exhaust.select :close SS.hold @08:06:06.43 Calibrating pressure and temperature sensors @08:06:06.44 CTV.dial :air @08:06:06.45 SSV.dial :spit,avoiding => exhaust @08:06:06.47 SS.reconfigure SSfast @08:06:06.50 SS.seek 5.00ml @08:06:09.23 SSV.dial CBV @08:06:09.24
CBV.dial SSV @08:06:10.58 delay 3.8 seconds @08:06:14.46 SSV.dial :spit @08:06:15.78 SS.seek 0.00ml @08:06:18.36 SSV.dial CBV @08:06:19.69 CTV.dial :intake @08:06:20.44 Waiting for external sampler to finish priming... @08:06:20.45 delayUntil "08:16:06.25" @08:06:51.80 delay 1 second @08:06:52.81 AFV.select :holdingCoil @08:06:52.90 delay 1 second @08:06:53.91 ATV.dial :waste @08:06:55.33 AS.reconfigure ASnorm @08:06:55.36 AS.home.seek empty @08:07:48.52 ATV.dial :waste @08:07:48.53 ARV.dial between carrier and air1 @08:07:48.60 ASV.dial :waste1 @08:16:06.26 SSV.dial CBV @08:16:06.28
CBV.dial SSV @08:16:06.29 Allowing 55s for 7.0ml of remaining 7.0ml @08:16:06.30 Intake.select :open @08:16:08.43 Intake @15.3psia, vacuum from 8.8 to 12.8psi @08:16:08.44 SS.reconfigure forSampling @08:16:08.48 SS.seek 7.00ml @08:16:13.69 Stroke completed -- waiting for pressure to equalize ... @08:16:54.12 SS.reconfigure SSconfig @08:16:54.15 SS.hold @08:16:54.24 Intake.select :close @08:16:54.38 ShallowSampler::Clogged in MAIN -- Intake @15psia & Outlet @4psia won't equalize after 7.0ml SSV.dial :exhaust @08:16:55.15 Exhaust.select :open @08:16:55.26 SS.reconfigure SSexhale @08:16:55.30 SS.seek 0.00ml @08:16:59.11 Exhaust.select :close @08:16:59.21 Sampling 1000.0ml @08:16:59.22 Intake.select :close @08:16:59.23 Exhaust.select :close @08:16:59.24 SS.reconfigure SSconfig @08:16:59.27 SS.hold @08:16:59.42 CTV.dial :intake @08:16:59.43 SSV.dial CBV @08:16:59.45
CBV.dial SSV @08:17:00.23 Allowing 185s for 25.0ml of remaining 1000.0ml Intake.select :open @08:17:02.36 Intake @15.2psia, vacuum from 8.7 to 12.7psi @08:17:02.37 SS.reconfigure forSampling @08:17:02.41 SS.seek 25.00ml @08:17:04.36 Vacuum too high -- waiting up to 40s @08:17:44.38 SS.reconfigure SSconfig @08:17:44.41 SS.hold @08:17:44.50 Intake.select :close @08:17:44.64 ShallowSampler::Clogged in MAIN -- Intake @15.1psia, & Outlet @2.2psia -- no progress after 1.3ml SSV.dial :exhaust @08:17:45.39 Exhaust.select :open @08:17:45.50 SS.reconfigure SSexhale @08:17:45.54 SS.seek 0.00ml @08:17:47.31 Exhaust.select :close @08:17:47.41 Sampled 1.3ml @08:17:47.42 collectSample: Complete @08:17:47.43 Evacuating Puck (bubblePt=55.0psi, delay=15.0s) Intake.select :close @08:17:47.49 CSV.dial :waste @08:17:47.52 CS.seek 0.000ml @08:17:47.61 SSV.dial CBV,avoiding => exhaust @08:17:47.64
CBV.dial SSV @08:17:47.68 CSV.dial :air @08:17:48.48 CS.seek 10.000ml @08:17:49.54
CTV.dial :air @08:17:50.30 delay 15 seconds @08:17:50.40 Sleepy.power :sampler,:OFF @08:18:05.30
SSV.dial :spit,avoiding => exhaust @08:18:07.31 CTV.dial CSV,avoiding => intake @08:18:07.33
SS.seek 0.00ml @08:18:07.43 SSV.dial CBV,avoiding => exhaust @08:18:12.58 CBV.dial between CSV and waste2b @08:18:13.52 CTV.dial CSV,avoiding => air @08:18:13.54
CSV.dial CTV @08:18:13.98 CS.reconfigure CSpressurize @08:18:14.01 CS.seek 0.000ml @08:18:39.24 BubblePoint: Intake @59.5psia, Outlet @4.0psia @08:18:39.25 SS.reconfigure SSconfig @08:18:39.28 SS.seek 10.00ml @08:18:44.81 delay 15 seconds @08:18:59.91 Evacuated: Intake @58.4psia, Outlet @-0.2psia @08:18:59.92 SSV.dial :spit,avoiding => exhaust @08:19:01.24 SS.reconfigure SSexhale @08:19:01.27 SS.seek 0.00ml @08:19:06.46 SS.reconfigure SSfast @08:19:06.50 SS.seek 5.00ml @08:19:09.02 SSV.dial CBV,avoiding => exhaust @08:19:09.04
CBV.dial SSV @08:19:10.39 CTV.dial :air,avoiding => intake @08:19:11.70 CSV.dial CTV @08:19:11.71
SS.reconfigure SSconfig @08:19:11.75 SS.seek 10.00ml @08:19:12.71 CSV.dial :air @08:19:13.43 CS.reconfigure CSfast @08:19:13.46 CS.seek 0.000ml @08:19:14.84 CS.coast @08:19:15.24
SSV.dial :spit,avoiding => exhaust @08:19:16.56 SS.reconfigure SSexhale @08:19:16.59 SS.seek 0.00ml @08:19:21.69 SS.reconfigure SSfast @08:19:21.73 SS.seek 5.00ml @08:19:24.25 SSV.dial CBV,avoiding => exhaust @08:19:24.27
CBV.dial SSV @08:19:26.58 SSV.dial :spit,avoiding => exhaust @08:19:27.91 SS.reconfigure SSconfig @08:19:27.94 SS.seek 0.00ml @08:19:31.29 SS.coast @08:19:31.32 Evacuating Puck (bubblePt=55.0psi, delay=15.0s) Intake.select :close @08:19:31.38 CSV.dial :waste @08:19:31.48 SSV.dial CBV,avoiding => exhaust @08:19:31.50
CBV.dial SSV @08:19:32.21 CS.seek 0.000ml @08:19:32.25 CSV.dial :air @08:19:32.81
CTV.dial :air @08:19:32.82 delay 15 seconds @08:19:33.04 CS.seek 10.000ml @08:19:47.82
SSV.dial :spit,avoiding => exhaust @08:19:49.12 CTV.dial CSV,avoiding => intake @08:19:49.14
SS.reconfigure SSexhale @08:19:49.20 SS.seek 0.00ml @08:19:49.26 SSV.dial CBV,avoiding => exhaust @08:19:56.83 CBV.dial between CSV and waste2b @08:19:57.77 CSV.dial between air and CTV @08:19:58.09 CS.seek 8.239ml @08:20:02.95 CTV.dial CSV,avoiding => air @08:20:02.96
CSV.dial CTV @08:20:03.26 CS.reconfigure CSpressurize @08:20:03.29 CS.seek 0.000ml @08:20:24.72 CSV.dial :air @08:20:25.24 CS.reconfigure CSfast @08:20:25.27 CS.seek 10.000ml @08:20:48.75 CSV.dial between air and CTV @08:20:49.15 CS.seek 3.075ml @08:21:05.76 CTV.dial CSV,avoiding => air @08:21:05.77
CSV.dial CTV @08:21:06.07 CS.reconfigure CSpressurize @08:21:06.10 CS.seek 0.000ml @08:21:13.42 BubblePoint: Intake @69.5psia, Outlet @14.1psia @08:21:13.43 SS.reconfigure SSconfig @08:21:13.46 SS.seek 10.00ml @08:21:19.25 delay 15 seconds @08:21:34.35 Evacuated: Intake @67.8psia, Outlet @0.1psia @08:21:34.36 SSV.dial :spit,avoiding => exhaust @08:21:35.68 SS.reconfigure SSexhale @08:21:35.71 SS.seek 0.00ml @08:21:40.64 SS.reconfigure SSfast @08:21:40.68 SS.seek 5.00ml @08:21:43.57 SSV.dial CBV,avoiding => exhaust @08:21:43.59
CBV.dial SSV @08:21:44.92 CTV.dial :air,avoiding => intake @08:21:46.21 CSV.dial CTV @08:21:46.23
SS.reconfigure SSconfig @08:21:46.27 SS.seek 10.00ml @08:21:47.23 CSV.dial :air @08:21:47.74 CS.reconfigure CSfast @08:21:47.77 CS.seek 0.000ml @08:21:49.41
SSV.dial :spit,avoiding => exhaust @08:21:49.46 CS.coast @08:21:50.73
SS.reconfigure SSexhale @08:21:50.76 SS.seek 0.00ml @08:21:56.12 SS.reconfigure SSfast @08:21:56.16 SS.seek 5.00ml @08:21:58.77 SSV.dial CBV,avoiding => exhaust @08:21:58.79
CBV.dial SSV @08:22:01.11 SSV.dial :spit,avoiding => exhaust @08:22:02.43 SS.reconfigure SSconfig @08:22:02.46 SS.seek 0.00ml @08:22:05.62 SS.coast @08:22:05.65 Lysing: Adding 1.5ml lysis @08:22:05.66 CBV.dial between CSV and waste1,avoiding => DBV @08:22:07.14 CTV.dial CSV @08:22:08.40 CSV.dial CSR @08:22:09.18 CSR.select :lysis @08:22:09.28 CS.reconfigure CSconfig @08:22:09.32 CS.seek 1.000ml @08:22:12.71 delay 5 seconds @08:22:17.72 CSR.select :air @08:22:17.86 CS.seek 6.000ml @08:22:31.30 delay 2 seconds @08:22:33.31 CSV.dial :waste1 @08:22:34.56 CS.reconfigure CSfast @08:22:34.59 CS.seek empty @08:22:49.02 CSV.dial CSR @08:22:50.30 CSR.select :lysis @08:22:50.40 CS.reconfigure CSconfig @08:22:50.44 CS.seek 1.500ml @08:22:55.15 delay 5 seconds @08:23:00.16 CSR.select :air @08:23:00.30 CS.seek 4.500ml @08:23:08.74 delay 2 seconds @08:23:10.75 CSV.dial CTV @08:23:11.27 CS.seek 1.275ml @08:23:20.31 Heating sh1 puck from tube 2 in Collection Clamp to 85.0C for 5:00 @08:23:20.33 CH.seek 85.0C,8:00 @08:25:39.42 CH.hold 5:00,85.0C @08:30:39.54 Collection Puck Heater at 85.0C after lysing @08:30:39.56 CS.seek 0.000ml @08:30:43.65 CTV.dial between spare and air,avoiding => intake @08:30:44.66 CSV.dial :air @08:30:45.10 CS.seek 5.000ml @08:30:58.54 CSV.dial CTV @08:30:59.06 delay 2 seconds @08:31:01.07 CSV.dial :air @08:31:01.59 delay 2 seconds @08:31:03.60 CSV.dial CTV @08:31:04.32 delay 2 seconds @08:31:06.33 CSV.dial :air @08:31:06.75 delay 2 seconds @08:31:08.76 CS.seek 0.000ml @08:31:22.23 Recovering Lysate into Collection syringe @08:31:22.24 CSV.dial CBV @08:31:23.23 CBV.dial CSV @08:31:23.59 CS.seek 5.000ml @08:31:37.10 delay 5 seconds @08:31:42.11 CTV.dial :air @08:31:42.57 CS.seek 7.000ml @08:31:48.48 delay 5 seconds @08:31:53.49 CSV.dial :air2 @08:31:53.93 CS.seek 10.000ml @08:32:02.35 CSV.dial between air and CTV,avoiding => CBV @08:32:03.49 Lysing Complete @08:32:03.54 Storing sh1 puck in Collection Clamp to tube 1 @08:32:03.67 SC.reconfigure SCtight @08:32:03.70 SC.seek 1 @08:32:05.95 SC.reconfigure SCloose @08:32:06.13 Elbow.reconfigure TightElbow @08:32:06.16 Elbow.seek 1 @08:32:07.80 Forearm.reconfigure TightForearm @08:32:07.83 Forearm.seek 1 + 800 counts @08:32:08.91 SE.reconfigure SEpick @08:32:08.96 SE.seek beamBreak @08:32:10.82 SE.configure SEconfig @08:32:10.94 Elbow.reconfigure LimpElbow @08:32:10.97 Forearm.reconfigure LooseForearm @08:32:11.01 Forearm.seek 1 @08:32:11.22 Elbow.hold @08:32:11.27 Elbow.reconfigure TightElbow @08:32:11.35 SE.seek 11.05 Pucks below Top Plate @08:32:11.67 Forearm.coast @08:32:11.71 Forearm.seek 1 + 326 counts @08:32:11.95 Forearm.reconfigure TightForearm @08:32:11.98 Forearm.seek 1 + 800 counts @08:32:12.25 SE.seek 12.05 Pucks below Top Plate @08:32:12.83 Forearm.reconfigure LooseForearm @08:32:12.86 Forearm.seek retract @08:32:13.81 Elbow.seek CC @08:32:15.35 Elbow.reconfigure LooseElbow @08:32:15.43 Forearm.seek CC @08:32:16.39 CC.seek sealed @08:32:17.32 Hand.grip @08:32:18.09 CC.seek open @08:32:21.43 Forearm.seek retract @08:32:22.38 Elbow.reconfigure TightElbow @08:32:22.42 Elbow.seek 1 @08:32:24.02 Elbow.reconfigure LimpElbow @08:32:24.07 Forearm.seek 1 @08:32:25.07 Elbow.hold @08:32:25.13 Elbow.reconfigure TightElbow @08:32:25.17 Hand.release @08:32:26.05 Forearm.coast @08:32:26.08 Forearm.seek 1 + 320 counts @08:32:26.32 Forearm.reconfigure TightForearm @08:32:26.35 Forearm.seek 1 + 800 counts @08:32:26.76 SE.seek down @08:32:28.34 SE.coast @08:32:28.47
Forearm.reconfigure LooseForearm @08:32:28.51 Forearm.seek retract @08:32:29.43 SC.coast @08:32:29.46 Tranferring lysate to PRV @08:32:29.47 CSV.dial :air @08:32:29.75 CS.seek 10.000ml @08:32:29.82 CSV.dial between air and CTV @08:32:30.19 AIV.dial :bypass @08:32:30.35 Beginning samplePassC @08:32:30.36 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:32:30.52 Elbow.seek FlushPuck::Garage @08:32:32.27 Elbow.reconfigure LooseElbow @08:32:32.35 Forearm.seek garage @08:32:33.36 Hand.grip @08:32:34.18 Forearm.seek retract @08:32:35.20 Elbow.reconfigure TightElbow @08:32:35.23 Elbow.seek CC @08:32:38.07 Elbow.reconfigure LooseElbow @08:32:38.15 Forearm.reconfigure TightForearm @08:32:38.18 Forearm.seek CC @08:32:39.28 CC.seek closed @08:32:43.05 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 2.01 @08:32:43.12 Hand.release @08:32:43.88 Forearm.reconfigure LooseForearm @08:32:43.91 Forearm.seek retract @08:32:44.88 AWV.select :air @08:32:44.89 AIV.dial :bypass @08:32:44.90 PSV.dial PSR @08:32:46.20 PSR.select :flush @08:32:46.30 PS.seek 1.500ml @08:33:02.33 delay 5 seconds @08:33:07.34 PSV.dial :waste1 @08:33:07.79 PS.seek 0.000ml @08:33:23.92 PSV.dial PSR @08:33:24.37 PSR.select :air @08:33:24.47 PS.seek 2.500ml @08:33:50.74 CBV.dial PRV @08:33:50.75 PRV.dial CBV @08:33:51.52
PSV.dial :mixloop @08:33:52.65 CTV.dial :air,avoiding => intake @08:33:52.66 PTV.dial :PRVmixing @08:33:52.73 PS.seek 0.000ml @08:34:18.98 PSV.dial :air @08:34:19.44 PS.seek 2.500ml @08:34:45.61 PSV.dial :mixloop @08:34:46.05 PS.seek 0.000ml @08:35:12.29 PSV.dial :air @08:35:12.76 PS.seek 2.500ml @08:35:39.04 PSV.dial :mixloop @08:35:39.61 PS.seek 0.000ml @08:36:05.85 Garaging FlushPuck in Collection Clamp @08:36:05.91 Elbow.reconfigure TightElbow @08:36:05.94 Elbow.seek CC @08:36:06.20 Elbow.reconfigure LooseElbow @08:36:06.28 Forearm.seek CC @08:36:07.22 CC.seek sealed @08:36:08.15 Hand.grip @08:36:08.83 CC.seek open @08:36:12.12 Forearm.seek retract @08:36:13.06 Elbow.reconfigure TightElbow @08:36:13.10 Elbow.seek garage @08:36:15.92 Elbow.reconfigure LooseElbow @08:36:16.00 Forearm.reconfigure TightForearm @08:36:16.03 Forearm.seek garage @08:36:17.17 Hand.release @08:36:18.00 Forearm.reconfigure LooseForearm @08:36:18.03 Forearm.seek retract @08:36:19.03 Connecting PS to CS to transfer sample @08:36:19.04 CBV.dial AIV @08:36:19.05 AIV.dial :bypass @08:36:19.06 PSV.dial :mixloop @08:36:19.07 PTV.dial :PRVmixing PRV.dial AIV @08:36:19.08 CTV.dial :air,avoiding => intake @08:36:19.09 Loading lyfil puck from tube 2 into Collection Clamp @08:36:19.87 SC.reconfigure SCtight @08:36:19.90 SC.seek 2 @08:36:22.12 SC.reconfigure SCloose @08:36:22.30 Elbow.reconfigure TightElbow @08:36:22.33 Elbow.seek 2 @08:36:24.19 Forearm.reconfigure TightForearm @08:36:24.22 Forearm.seek 2 + 800 counts @08:36:25.29 SE.reconfigure SEpick @08:36:25.34 SE.seek beamBreak @08:36:27.48 SE.configure SEconfig @08:36:27.60 Elbow.seek 2 @08:36:27.74 Forearm.seek 2 + 300 counts @08:36:28.04 Forearm.reconfigure LooseForearm @08:36:28.08 Hand.grip @08:36:28.84 SE.seek down @08:36:28.98
Forearm.seek retract @08:36:29.97 Elbow.seek CC @08:36:30.83 SE.coast @08:36:31.54
Elbow.reconfigure LooseElbow @08:36:31.62 Forearm.reconfigure TightForearm @08:36:31.65 Forearm.seek CC @08:36:32.77 CC.seek closed @08:36:36.52 Collection Clamp onSpring/offSpring for 5/22 samples, on/off torque 2.00 @08:36:36.53 Hand.release @08:36:37.37 Forearm.reconfigure LooseForearm @08:36:37.40 Forearm.seek retract @08:36:38.34 SC.coast @08:36:38.37 CTV.dial CSV,avoiding => intake @08:36:39.68 CSV.dial CTV @08:36:39.99 CS.seek 5.000ml @08:36:53.53 delay 1:20 @08:38:13.54 CS.seek 2.500ml @08:38:20.62 delay 40 seconds @08:39:00.63 CS.seek 0.000ml @08:39:07.96 delay 1:00 @08:40:07.97 PSV.dial :air @08:40:08.40 PS.seek 2.500ml @08:40:34.59 PSV.dial :mixloop @08:40:35.03 delay 3 seconds @08:40:38.04 PSV.dial :air @08:40:38.48 delay 2 seconds @08:40:40.49 PSV.dial :mixloop @08:40:40.92 delay 3 seconds @08:40:43.93 PSV.dial :waste1 @08:40:45.23 PS.seek empty @08:41:11.50 CBV.dial :waste1 @08:41:11.97 delay 5 seconds @08:41:16.98 CBV.dial AIV @08:41:17.44 delay 10 seconds @08:41:27.45 CBV.dial :waste1 @08:41:27.92 delay 10 seconds @08:41:37.93 CBV.dial AIV @08:41:38.39 delay 10 seconds @08:41:48.40 CTV.dial CSV,avoiding => intake @08:41:48.41 CBV.dial between AIV and waste1 @08:41:48.73 CSV.dial :air @08:41:49.26 CS.seek 10.000ml @08:42:15.33 CSV.dial CTV @08:42:15.85 CS.seek 0.000ml @08:42:41.95 CBV.dial :waste1 @08:42:42.26 delay 10 seconds @08:42:52.27 CTV.dial :air,avoiding => intake @08:42:53.63 Storing lyfil puck in Collection Clamp to tube 1 @08:42:53.76 SC.reconfigure SCtight @08:42:53.79 SC.seek 1 @08:42:56.02 SC.reconfigure SCloose @08:42:56.20 Elbow.reconfigure TightElbow @08:42:56.23 Elbow.seek 1 @08:42:57.88 Forearm.reconfigure TightForearm @08:42:57.91 Forearm.seek 1 + 800 counts @08:42:58.89 SE.reconfigure SEpick @08:42:58.94 SE.seek beamBreak @08:43:00.73 SE.configure SEconfig @08:43:00.85 Elbow.reconfigure LimpElbow @08:43:00.88 Forearm.reconfigure LooseForearm @08:43:00.92 Forearm.seek 1 @08:43:01.15 Elbow.hold @08:43:01.20 Elbow.reconfigure TightElbow @08:43:01.28 SE.seek 12.05 Pucks below Top Plate @08:43:01.62 Forearm.coast @08:43:02.23 Forearm.seek 1 + 326 counts @08:43:02.47 Forearm.reconfigure TightForearm @08:43:02.50 Forearm.seek 1 + 800 counts @08:43:02.82 SE.seek 13.05 Pucks below Top Plate @08:43:03.33 Forearm.reconfigure LooseForearm @08:43:03.36 Forearm.seek retract @08:43:04.28 Elbow.seek CC @08:43:05.82 Elbow.reconfigure LooseElbow @08:43:05.90 Forearm.seek CC @08:43:06.86 CC.seek sealed @08:43:07.79 Hand.grip @08:43:08.57 CC.seek open @08:43:11.93 Forearm.seek retract @08:43:12.88 Elbow.reconfigure TightElbow @08:43:12.92 Elbow.seek 1 @08:43:14.54 Elbow.reconfigure LimpElbow @08:43:14.59 Forearm.seek 1 @08:43:15.54 Elbow.hold @08:43:15.59 Elbow.reconfigure TightElbow @08:43:15.63 Hand.release @08:43:16.40 Forearm.coast @08:43:16.43 Forearm.seek 1 + 341 counts @08:43:16.68 Forearm.reconfigure TightForearm @08:43:16.71 Forearm.seek 1 + 800 counts @08:43:16.99 SE.seek down @08:43:18.09
Forearm.reconfigure LooseForearm @08:43:18.13 Forearm.seek retract @08:43:18.40 SE.coast @08:43:19.03
SC.coast @08:43:19.06 PSV.dial :air @08:43:20.13 PS.seek 0.499ml @08:43:26.04 delay 3 seconds @08:43:29.06 PSV.dial :mixloop @08:43:29.50 PRV.dial :air @08:43:29.94 delay 3 seconds @08:43:32.95 PRV.dial CBV @08:43:33.38 CBV.dial AIV @08:43:33.83 PRV.dial between CBV and air @08:43:34.13 PRV.dial :air @08:43:34.48 PS.reconfigure PSslow @08:43:34.51 PS.seek 0.599ml @08:43:37.10 PRV.dial :waste2 @08:43:37.83 PS.reconfigure PSconfig @08:43:37.86 PS.seek empty @08:43:44.78 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:43:44.93 Elbow.seek FlushPuck::Garage @08:43:46.65 Elbow.reconfigure LooseElbow @08:43:46.74 Forearm.seek garage @08:43:47.77 Hand.grip @08:43:48.54 Forearm.seek retract @08:43:49.55 Elbow.reconfigure TightElbow @08:43:49.59 Elbow.seek CC @08:43:52.35 Elbow.reconfigure LooseElbow @08:43:52.43 Forearm.reconfigure TightForearm @08:43:52.46 Forearm.seek CC @08:43:53.52 CC.seek closed @08:43:57.33 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 2.03 @08:43:57.38 Hand.release @08:43:58.11 Forearm.reconfigure LooseForearm @08:43:58.14 Forearm.seek retract @08:43:59.13 Clearing PML for modified lysate @08:43:59.14 CTV.dial :air,avoiding => intake @08:43:59.15 PRV.dial :waste2 PTV.dial :PRV,avoiding => puck @08:43:59.89 delay 2 seconds @08:44:01.89 PTV.dial :PRVmixing,avoiding => puck @08:44:02.61 delay 2 seconds @08:44:04.62 PSV.dial :PML @08:44:04.63 delay 2 seconds @08:44:07.18 PRV.dial :air @08:44:07.89 delay 2 seconds @08:44:09.95 PS.seek 1.499ml @08:44:26.06 delay 5 seconds @08:44:31.07 PS.seek 2.500ml @08:44:42.03 PSV.dial :air @08:44:42.48 PS.seek 0.000ml @08:45:08.69 mixing lysate with speDil @08:45:08.70 PSV.dial :PML @08:45:09.14 CBV.dial :PRV @08:45:09.15 PRV.dial :air @08:45:09.16 delay 2 seconds @08:45:11.18 PRV.dial :speDil @08:45:11.63 delay 2 seconds @08:45:13.69 PS.reconfigure PSslow1 @08:45:13.72 PS.seek 0.225ml @08:45:37.09 delay 5 seconds @08:45:42.10 PRV.dial CBV @08:45:42.83 delay 2 seconds @08:45:44.89 PS.seek 0.475ml @08:46:10.65 delay 5 seconds @08:46:15.66 PRV.dial :air @08:46:16.09 delay 2 seconds @08:46:18.10 PS.seek 1.000ml @08:47:11.72 delay 10 seconds @08:47:21.73 PRV.dial CBV @08:47:22.18 CTV.dial :air,avoiding => intake @08:47:22.19 PSV.dial :air @08:47:22.77 PS.reconfigure PSconfig @08:47:22.80 PS.seek 2.500ml @08:47:38.93 delay 3 seconds @08:47:41.94 PSV.dial PTV @08:47:42.65 PTV.dial :PRV,avoiding => puck @08:47:43.38 PS.seek 0.000ml @08:48:09.65 PSV.dial :air @08:48:10.39 PS.seek 2.500ml @08:48:36.68 delay 3 seconds @08:48:39.69 PSV.dial PTV @08:48:40.41 PTV.dial :PRV,avoiding => puck @08:48:40.43 PS.seek 0.000ml @08:49:06.90 Pushing modified lysate into AIV Injection Loop @08:49:06.91 CBV.dial AIV @08:49:06.92 AIV.dial :bypass @08:49:06.93 PRV.dial CBV @08:49:06.94 PSV.dial :air @08:49:07.67 PS.seek 2.500ml @08:49:33.90 delay 3 seconds @08:49:36.91 PSV.dial :PML @08:49:37.35 PTV.dial :PRVmixing,avoiding => puck @08:49:38.14 PS.reconfigure PSslow1 @08:49:38.17 PS.seek 1.740ml @08:50:55.83 delay 5 seconds @08:51:00.84 AIV.dial :sample @08:51:01.38 AWV.select :waste @08:51:01.53 PS.reconfigure PSslow2 @08:51:01.56 PS.seek 1.220ml @08:52:47.73 delay 15 seconds @08:53:02.74 AIV.dial :bypass @08:53:03.30 PS.reconfigure PSconfig @08:53:03.33 PS.seek 0.000ml @08:53:16.51 PSV.dial :air @08:53:17.09 PS.seek 2.500ml @08:53:43.39 PSV.dial :PML @08:53:43.82 PS.seek 0.000ml @08:54:10.04 AIV.dial :coilToMFB @08:54:10.05 AWV.select :air @08:54:10.19 Extracting Nucleic Acid from AIV @08:54:10.21
Beginning samplePassD @08:54:10.22 SPE.hold 2:46:40,55.0C @08:54:10.24 ASV.dial :SPEdelivery @08:54:10.36
AWV.select :air @08:54:10.37 AIV.dial :bypass @08:54:10.38 PSV.dial PSR @08:54:10.80 delay 20 seconds @08:54:11.40
PSR.select :killMFB @08:54:11.50 PS.seek 1.500ml @08:54:27.64 delay 5 seconds @08:54:30.81 primeLysateINT @08:54:30.82 AWV.select :air @08:54:30.83 AIV.dial :coilToMFB ATV.dial :AIV @08:54:32.18 AS.reconfigure ASslow1 @08:54:32.21 AS.seek 0.241ml @08:54:32.65
PSV.dial :waste1 @08:54:33.10 PS.seek 0.000ml @08:54:49.20 PSV.dial PSR @08:54:49.65 PSR.select :air @08:54:49.75 PS.seek 2.500ml @08:55:15.95 PRV.dial CBV @08:55:15.97
CBV.dial PRV @08:55:15.98 PSV.dial :mixloop @08:55:17.01 CTV.dial :air,avoiding => intake @08:55:17.02 PTV.dial :PRVmixing @08:55:17.04 PS.seek 0.000ml @08:55:21.88 delay 10 seconds @08:55:31.89 ATV.dial :air2 @08:55:32.47 AS.seek 0.271ml @08:55:38.83 ATV.dial :waste2 @08:55:39.18 AS.reconfigure ASnorm @08:55:39.21 AS.seek empty @08:55:43.32
PSV.dial PSR @08:55:44.37 PSR.select :flush @08:55:44.47 PS.seek 1.500ml @08:55:53.50 Lysate primed! @08:55:53.51 AFV.select :holdingCoil @08:55:53.52 ATV.dial :air2 @08:55:53.91 AS.reconfigure ASslow1 @08:55:54.47 AS.seek 0.069ml @08:56:00.51
delay 5 seconds @08:56:05.52 PSV.dial :waste1 @08:56:05.97 PS.seek 0.000ml @08:56:09.03 delay 3 seconds @08:56:12.04 ATV.dial :AIV @08:56:12.63 AS.seek 0.469ml @08:56:22.07
PSV.dial PSR @08:56:22.52 PSR.select :air @08:56:22.62 PS.seek 2.500ml @08:56:48.91 PRV.dial CBV @08:56:48.93
CBV.dial PRV @08:56:48.94 PSV.dial :mixloop @08:56:50.07 CTV.dial :air,avoiding => intake @08:56:50.08 PTV.dial :PRVmixing @08:56:50.10 PS.seek 0.000ml @08:57:16.54 PSV.dial PSR @08:57:17.55 PSR.select :flush @08:57:17.65 PS.seek 1.500ml @08:57:33.76 delay 5 seconds @08:57:34.47 delay 5 seconds @08:57:38.77
PSV.dial :waste1 @08:57:39.22 PS.seek 0.000ml @08:57:39.52 Solid Phase Extraction Heater at 51.4C @08:57:39.53 ATV.dial ASV @08:57:39.87 ASV.dial :SPEdelivery @08:57:39.93 AS.seek 0.041ml @08:57:55.29
PSV.dial PSR @08:57:55.75 PSR.select :air @08:57:55.84 PS.seek 2.500ml @08:58:22.07 PRV.dial CBV @08:58:22.09
CBV.dial PRV @08:58:22.10 PSV.dial :mixloop @08:58:23.10 CTV.dial :air,avoiding => intake @08:58:23.11 PTV.dial :PRVmixing @08:58:23.13 PS.seek 0.000ml @08:58:49.38 PSV.dial PSR @08:58:50.39 PSR.select :flush @08:58:50.49 PS.seek 1.500ml @08:59:06.66 delay 5 seconds @08:59:07.49 delay 10 seconds @08:59:11.67
PSV.dial :waste1 @08:59:12.12 PS.seek 0.000ml @08:59:17.50 rinseLysateLineINT @08:59:17.51 AFV.select :carrierSolution @08:59:17.60 delay 2 seconds @08:59:19.61 AS.reconfigure ASconfig @08:59:19.64 AS.seek 1.000ml @08:59:28.19
PSV.dial PSR @08:59:28.64 PSR.select :air @08:59:28.74 PS.seek 2.500ml @08:59:55.00 PRV.dial CBV @08:59:55.02
CBV.dial PRV @08:59:55.03 PSV.dial :mixloop @08:59:56.03 CTV.dial :air,avoiding => intake @08:59:56.04 PTV.dial :PRVmixing @08:59:56.06 PS.reconfigure PSslow @08:59:56.09 PS.seek 1.200ml @09:00:15.80 delay 1 second @09:00:16.81 AFV.select :holdingCoil @09:00:16.90 delay 1 second @09:00:17.91 ATV.dial :AIV @09:00:18.37 AIV.dial :coilToMFB @09:00:18.38 AWV.select :waste @09:00:18.48 AS.reconfigure ASslow0 @09:00:18.52 AS.seek 0.000ml @09:00:23.07
PSV.dial :waste1 @09:00:24.38 PS.reconfigure PSconfig @09:00:24.41 PS.seek 0.000ml @09:00:37.45 PSV.dial :air @09:00:38.47 PS.seek 2.500ml @09:01:04.63 PSV.dial :mixloop @09:01:05.08 PS.reconfigure PSslow @09:01:05.11 PS.seek 0.000ml @09:01:56.44 PSV.dial :air @09:01:56.91 PS.reconfigure PSconfig @09:01:56.94 PS.seek 2.500ml @09:02:00.86 ATV.dial :air2 @09:02:01.44 AS.reconfigure ASslow1 @09:02:01.47 AS.seek 0.050ml @09:02:11.95 ATV.dial :AIV @09:02:12.53 AS.seek 0.010ml @09:02:20.94 ATV.dial :waste @09:02:22.32 AS.seek empty @09:02:23.16
PSV.dial :mixloop @09:02:23.61 PS.reconfigure PSslow @09:02:23.64 PS.seek 0.000ml @09:02:24.73 ethanol wash @09:02:24.74 ATV.dial :air2 @09:02:25.70 AS.reconfigure ASslow2 @09:02:25.73 AS.seek 0.009ml @09:02:36.31 delay 3 seconds @09:02:39.32 Pulling 0.4ml of Ethanol Wash @09:02:39.33 ATV.dial :ethanol @09:02:39.91 AS.reconfigure ASslow1 @09:02:39.94 AS.seek 0.409ml @09:03:14.91
Cleaning Collection Syringe @09:03:14.92 CSV.dial CSR @09:03:15.35 CSR.select :flush @09:03:15.45 CS.seek 4.000ml @09:03:26.39 delay 5 seconds @09:03:31.40 CSR.select :air @09:03:31.49 CS.seek 10.000ml @09:03:47.48 CSV.dial CTV @09:03:47.50
CTV.dial CSV @09:03:48.84 CBV.dial :waste1 @09:03:49.32 CS.seek 0.000ml @09:04:01.78 delay 3 seconds @09:04:04.79 ATV.dial :air1 @09:04:05.62 delay 3 seconds @09:04:08.68 AS.seek 0.434ml @09:04:14.03 delay 3 seconds @09:04:15.42
CSV.dial CSR @09:04:16.34 CSR.select :flush @09:04:16.44 CS.seek 4.000ml @09:04:17.04 ATV.dial :ethanol @09:04:17.51 AS.seek 0.419ml @09:04:20.81 delay 3 seconds @09:04:23.82 ATV.dial ASV @09:04:24.97 AS.seek 0.092ml @09:04:27.45
delay 5 seconds @09:04:32.46 CSR.select :air @09:04:32.55 CS.seek 10.000ml @09:04:48.54 CSV.dial CTV @09:04:48.56
CTV.dial CSV @09:04:49.07 CBV.dial :waste1 @09:04:49.09 CS.seek 0.000ml @09:05:15.46 Garaging FlushPuck in Collection Clamp @09:05:15.51 Elbow.reconfigure TightElbow @09:05:15.55 Elbow.seek CC @09:05:15.65 Elbow.reconfigure LooseElbow @09:05:15.73 Forearm.seek CC @09:05:16.70 CC.seek sealed @09:05:17.63 Hand.grip @09:05:18.41 CC.seek open @09:05:21.72 Forearm.seek retract @09:05:22.67 Elbow.reconfigure TightElbow @09:05:22.70 Elbow.seek garage @09:05:25.51 Elbow.reconfigure LooseElbow @09:05:25.59 Forearm.reconfigure TightForearm @09:05:25.62 Forearm.seek garage @09:05:26.80 Hand.release @09:05:27.57 Forearm.reconfigure LooseForearm @09:05:27.60 Forearm.seek retract @09:05:28.63 Whole Cell Archive sampling 1000ml @09:05:28.65 Loading wcr puck from tube 2 into Collection Clamp @09:05:28.83 SC.reconfigure SCtight @09:05:28.86 SC.seek 2 @09:05:31.14 SC.reconfigure SCloose @09:05:31.32 Elbow.reconfigure TightElbow @09:05:31.35 Elbow.seek 2 @09:05:31.88 delay 3 seconds @09:05:33.18 Forearm.reconfigure TightForearm @09:05:33.21 Forearm.seek 2 + 800 counts @09:05:34.40 SE.reconfigure SEpick @09:05:34.45 SE.seek beamBreak @09:05:34.89 ATV.dial :waste2 @09:05:35.42 AS.reconfigure ASconfig @09:05:35.45 AS.seek empty @09:05:36.68 SE.configure SEconfig @09:05:36.81 Elbow.seek 2 @09:05:37.07 Forearm.seek 2 + 300 counts @09:05:37.32 Forearm.reconfigure LooseForearm @09:05:37.36 Hand.grip @09:05:38.17 SE.seek down @09:05:38.30 Forearm.seek retract @09:05:39.26 Elbow.seek CC @09:05:40.21 SE.coast @09:05:40.74 Elbow.reconfigure LooseElbow @09:05:40.82 Forearm.reconfigure TightForearm @09:05:40.85 Forearm.seek CC @09:05:41.15 ATV.dial :air2 @09:05:41.49 AS.reconfigure ASslow1 @09:05:41.52 AS.seek 1.000ml @09:05:41.90 CC.seek closed @09:05:45.66 Collection Clamp onSpring/offSpring for 4/21 samples, on/off torque 2.01 @09:05:45.67 Hand.release @09:05:47.05 Forearm.reconfigure LooseForearm @09:05:47.08 Forearm.seek retract @09:05:48.02 SC.coast @09:05:48.06 collectSample 1000ml w/bubble point of 55psi @09:05:48.07 Priming 7ml Sleepy.power :sampler,:ON @09:05:48.08 Intake.select :close @09:05:48.09 Exhaust.select :close @09:05:48.10 SS.hold @09:05:48.24 Calibrating pressure sensors @09:05:48.25 CTV.dial :air @09:05:49.56 SSV.dial :spit,avoiding => exhaust @09:05:49.57 SS.reconfigure SSfast @09:05:49.60 SS.seek 5.00ml @09:05:52.44 SSV.dial CBV @09:05:52.46 CBV.dial SSV @09:05:53.85 delay 3.8 seconds @09:05:57.75 SSV.dial :spit @09:05:59.10 SS.seek 0.00ml @09:06:01.54 SSV.dial CBV @09:06:02.85 CTV.dial :intake @09:06:03.57 Waiting for external sampler to finish priming... @09:06:03.58 delayUntil "09:15:48.07" @09:09:05.91 delay 5 seconds @09:09:10.92 ATV.dial ASV @09:09:11.64 ASV.dial :SPEdelivery @09:09:11.66 AS.seek 0.020ml @09:12:31.81 delay 5 seconds @09:12:36.82 ATV.dial :air2 @09:12:37.59 AS.seek 0.520ml @09:14:19.74 delay 5 seconds @09:14:24.75 ATV.dial ASV @09:14:25.46 ASV.dial :wasteR @09:14:26.00 AS.seek 0.300ml @09:15:11.09 delay 20 seconds @09:15:31.10 AS.seek 0.070ml @09:15:48.07 SSV.dial CBV @09:15:48.09 CBV.dial SSV @09:15:48.10 Allowing 55s for 7.0ml of remaining 7.0ml @09:15:48.11 Intake.select :open @09:15:50.24 Intake @33.3psia, vacuum from 20.0 to 24.0psi @09:15:50.26 SS.reconfigure forSampling @09:15:50.29 SS.seek 7.00ml @09:15:54.19 Vacuum too high -- waiting up to 40s @09:15:54.46 Intake @25.5psia, vacuum from 19.0 to 23.0psi @09:15:54.47 SS.reconfigure forSampling @09:15:54.50 SS.seek 7.00ml @09:15:55.93 Stroke completed -- waiting for pressure to equalize ... @09:15:58.34 Intake.select :close @09:15:58.48 SSV.dial :exhaust @09:15:59.24 Exhaust.select :open @09:15:59.34 SS.reconfigure SSexhale @09:15:59.38 SS.seek 0.00ml @09:16:03.28 Exhaust.select :close @09:16:03.39 Sampling 1000.0ml @09:16:03.40 Intake.select :close @09:16:03.41 Exhaust.select :close @09:16:03.42 SS.reconfigure SSconfig @09:16:03.45 SS.hold @09:16:03.60 CTV.dial :intake @09:16:03.61 SSV.dial CBV @09:16:03.63 CBV.dial SSV @09:16:04.43 Allowing 185s for 25.0ml of remaining 1000.0ml @09:16:04.44 Intake.select :open @09:16:06.57 Intake @34.7psia, vacuum from 20.0 to 24.0psi @09:16:06.59 SS.reconfigure forSampling @09:16:06.62 SS.seek 25.00ml @09:16:10.21 Vacuum too high -- waiting up to 40s @09:16:10.48 Intake @24.6psia, vacuum from 18.1 to 22.1psi @09:16:10.49 SS.reconfigure forSampling @09:16:10.53 SS.seek 25.00ml @09:16:18.19 delay 20 seconds @09:16:24.32 Stroke completed -- waiting for pressure to equalize ... @09:16:24.96 Intake.select :close @09:16:25.10 SSV.dial :exhaust @09:16:25.87 Exhaust.select :open @09:16:25.97 SS.reconfigure SSexhale @09:16:26.01 SS.seek 0.00ml @09:16:34.56 Exhaust.select :close @09:16:34.66 SSV.dial CBV @09:16:34.68 CBV.dial SSV @09:16:35.46 Allowing 185s for 25.0ml of remaining 975.0ml @09:16:35.47 Intake.select :open @09:16:37.60 Intake @34.6psia, vacuum from 20.0 to 24.0psi @09:16:37.62 SS.reconfigure forSampling @09:16:37.65 SS.seek 25.00ml @09:16:38.20 ATV.dial :waste2 @09:16:38.83 AS.reconfigure ASconfig @09:16:38.86 AS.seek empty @09:16:41.15 Vacuum too high -- waiting up to 40s @09:16:41.42 Intake @25.3psia, vacuum from 18.8 to 22.8psi @09:16:41.44 SS.reconfigure forSampling @09:16:41.47 SS.seek 25.00ml @09:16:43.24 Elution @09:16:43.25 ATV.dial :air1 @09:16:44.23 AS.reconfigure ASslow1 @09:16:44.26 AS.seek 0.049ml @09:16:54.75 delay 3 seconds @09:16:56.40 Stroke completed -- waiting for pressure to equalize ... @09:16:57.16 Intake.select :close @09:16:57.30 SSV.dial :exhaust @09:16:57.90 ATV.dial :ElutionWater @09:16:58.57 AS.seek 0.109ml @09:16:58.63 Exhaust.select :open @09:16:58.73 SS.reconfigure SSexhale @09:16:58.77 SS.seek 0.00ml @09:17:07.68 Exhaust.select :close @09:17:07.78 SSV.dial CBV @09:17:07.80 CBV.dial SSV @09:17:08.59 Allowing 185s for 25.0ml of remaining 950.0ml @09:17:08.61 Intake.select :open @09:17:10.74 Intake @35.3psia, vacuum from 20.0 to 24.0psi @09:17:10.76 SS.reconfigure forSampling @09:17:10.79 SS.seek 25.00ml @09:17:11.05 delay 3 seconds @09:17:14.00 Vacuum too high -- waiting up to 40s @09:17:14.06 ATV.dial ASV,via => air2 @09:17:14.27 Intake @27.0psia, vacuum from 20.0 to 24.0psi @09:17:14.29 SS.reconfigure forSampling @09:17:14.32 SS.seek 25.00ml @09:17:15.72 ASV.dial :SPEelution @09:17:16.28 AFV.select :carrierSolution @09:17:16.43 AS.reconfigure ASnorm @09:17:16.46 AS.seek 0.759ml @09:17:32.95 Stroke completed -- waiting for pressure to equalize ... @09:17:33.59 Intake.select :close @09:17:33.73 SSV.dial :exhaust @09:17:34.50 Exhaust.select :open @09:17:34.60 SS.reconfigure SSexhale @09:17:34.64 SS.seek 0.00ml @09:17:43.41 Exhaust.select :close @09:17:43.51 SSV.dial CBV @09:17:43.53 CBV.dial SSV @09:17:44.31 Allowing 185s for 25.0ml of remaining 925.0ml @09:17:44.32 Intake.select :open @09:17:46.46 Intake @35.3psia, vacuum from 20.0 to 24.0psi @09:17:46.48 SS.reconfigure forSampling @09:17:46.51 SS.seek 25.00ml @09:17:49.41 Vacuum too high -- waiting up to 40s @09:17:49.68 Intake @28.6psia, vacuum from 20.0 to 24.0psi @09:17:49.70 SS.reconfigure forSampling @09:17:49.73 SS.seek 25.00ml @09:17:49.95 delay 3 seconds @09:17:52.96 AFV.select :holdingCoil @09:17:53.11 AS.reconfigure ASslow1 @09:17:53.14 AS.seek 0.489ml @09:18:11.95 Stroke completed -- waiting for pressure to equalize ... @09:18:12.59 Intake.select :close @09:18:12.73 SSV.dial :exhaust @09:18:13.50 Exhaust.select :open @09:18:13.60 SS.reconfigure SSexhale @09:18:13.64 SS.seek 0.00ml @09:18:22.20 Exhaust.select :close @09:18:22.30 SSV.dial CBV @09:18:22.32 CBV.dial SSV @09:18:23.09 Allowing 185s for 25.0ml of remaining 900.0ml @09:18:23.11 Intake.select :open @09:18:25.24 Intake @34.9psia, vacuum from 20.0 to 24.0psi @09:18:25.26 SS.reconfigure forSampling @09:18:25.29 SS.seek 25.00ml @09:18:27.85 Vacuum too high -- waiting up to 40s @09:18:28.12 Intake @29.6psia, vacuum from 20.0 to 24.0psi @09:18:28.14 SS.reconfigure forSampling @09:18:28.17 SS.seek 25.00ml @09:18:45.19 Vacuum too high -- waiting up to 40s @09:18:45.67 Intake @28.3psia, vacuum from 20.0 to 24.0psi @09:18:45.68 SS.reconfigure forSampling @09:18:45.72 SS.seek 25.00ml @09:18:48.43 delay 5 seconds @09:18:53.49 AS.reconfigure ASslow2 @09:18:53.52 AS.seek 0.454ml @09:19:00.71 Stroke completed -- waiting for pressure to equalize ... @09:19:01.35 Intake.select :close @09:19:01.49 SSV.dial :exhaust @09:19:02.25 Exhaust.select :open @09:19:02.35 SS.reconfigure SSexhale @09:19:02.39 SS.seek 0.00ml @09:19:10.92 Exhaust.select :close @09:19:11.02 SSV.dial CBV @09:19:11.04 CBV.dial SSV @09:19:11.81 Allowing 185s for 25.0ml of remaining 875.0ml @09:19:11.83 Intake.select :open @09:19:13.96 Intake @33.5psia, vacuum from 20.0 to 24.0psi @09:19:13.98 SS.reconfigure forSampling @09:19:14.01 SS.seek 25.00ml @09:19:19.22 Vacuum too high -- waiting up to 40s @09:19:19.48 Intake @28.7psia, vacuum from 20.0 to 24.0psi @09:19:19.49 SS.reconfigure forSampling @09:19:19.53 SS.seek 25.00ml @09:19:29.49 delay 1:00 @09:20:11.60 Stroke completed -- waiting for pressure to equalize ... @09:20:13.41 Intake.select :close @09:20:13.55 SSV.dial :exhaust @09:20:14.32 Exhaust.select :open @09:20:14.42 SS.reconfigure SSexhale @09:20:14.46 SS.seek 0.00ml @09:20:23.01 Exhaust.select :close @09:20:23.11 SSV.dial CBV @09:20:23.67 CBV.dial SSV @09:20:24.47 Allowing 185s for 25.0ml of remaining 850.0ml @09:20:24.48 Intake.select :open @09:20:26.62 Intake @33.7psia, vacuum from 20.0 to 24.0psi @09:20:26.63 SS.reconfigure forSampling @09:20:26.66 SS.seek 25.00ml @09:20:29.50 pushing eluate to holding coil @09:20:29.56 AS.seek 0.294ml @09:20:44.62 Vacuum too high -- waiting up to 40s @09:20:44.88 Intake @30.0psia, vacuum from 20.0 to 24.0psi @09:20:44.90 SS.reconfigure forSampling @09:20:44.93 SS.seek 25.00ml @09:22:02.21 Stroke completed -- waiting for pressure to equalize ... @09:22:04.03 Intake.select :close @09:22:04.17 SSV.dial :exhaust @09:22:04.94 Exhaust.select :open @09:22:05.04 SS.reconfigure SSexhale @09:22:05.08 SS.seek 0.00ml @09:22:13.72 Exhaust.select :close @09:22:13.82 SSV.dial CBV @09:22:13.84 CBV.dial SSV @09:22:14.63 Allowing 185s for 25.0ml of remaining 825.0ml @09:22:14.64 Intake.select :open @09:22:16.78 Intake @31.2psia, vacuum from 20.0 to 24.0psi @09:22:16.80 SS.reconfigure forSampling @09:22:16.83 SS.seek 25.00ml @09:23:13.11 delay 30 seconds @09:23:43.12 ATV.dial :waste,via => air3 @09:23:44.59 AS.reconfigure ASconfig @09:23:44.62 AS.seek empty @09:24:02.08 Parking Bleach in LysateSource line and SPEcolumn @09:24:02.09 ATV.dial :air3 @09:24:02.68 AS.reconfigure ASslow1 @09:24:02.71 AS.seek 0.029ml @09:24:07.78 Stroke completed -- waiting for pressure to equalize ... @09:24:09.05 ATV.dial :ElutionWater @09:24:09.44 AS.seek 0.059ml @09:24:10.19 Intake.select :close @09:24:10.33 SSV.dial :exhaust @09:24:11.11 Exhaust.select :open @09:24:11.21 SS.reconfigure SSexhale @09:24:11.25 SS.seek 0.00ml @09:24:15.80 ATV.dial :air3 @09:24:16.27 AS.seek 0.069ml @09:24:18.47 delay 2 seconds @09:24:19.96 Exhaust.select :close @09:24:20.06 SSV.dial CBV @09:24:20.08 CBV.dial SSV @09:24:20.48 ATV.dial :bleach @09:24:20.87 AS.seek 0.711ml @09:24:20.91 Allowing 185s for 25.0ml of remaining 800.0ml @09:24:20.92 Intake.select :open @09:24:23.05 Intake @30.0psia, vacuum from 20.0 to 24.0psi @09:24:23.07 SS.reconfigure forSampling @09:24:23.10 SS.seek 25.00ml @09:26:10.10 Vacuum too high -- waiting up to 40s @09:26:11.02 Intake @27.3psia, vacuum from 20.0 to 24.0psi @09:26:11.04 SS.reconfigure forSampling @09:26:11.07 SS.seek 25.00ml @09:26:14.60 Stroke completed -- waiting for pressure to equalize ... @09:26:22.30 Intake.select :close @09:26:22.44 SSV.dial :exhaust @09:26:23.21 Exhaust.select :open @09:26:23.31 SS.reconfigure SSexhale @09:26:23.35 SS.seek 0.00ml @09:26:31.99 Exhaust.select :close @09:26:32.04 delay 3 seconds @09:26:32.09 SSV.dial CBV @09:26:32.11 CBV.dial SSV @09:26:32.91 Allowing 185s for 25.0ml of remaining 775.0ml @09:26:32.92 Intake.select :open @09:26:35.05 Intake @29.1psia, vacuum from 20.0 to 24.0psi @09:26:35.07 SS.reconfigure forSampling @09:26:35.09 ATV.dial :air3 @09:26:35.11 SS.seek 25.00ml @09:26:35.49 delay 3 seconds @09:26:38.54 AS.seek 0.736ml @09:26:43.90 delay 3 seconds @09:26:46.91 ATV.dial :bleach @09:26:47.30 AS.seek 0.721ml @09:26:51.22 delay 3 seconds @09:26:54.22 ATV.dial :AIV @09:26:55.41 AS.reconfigure ASconfig @09:26:55.44 AS.seek 0.015ml @09:26:58.54 Vacuum too high -- waiting up to 40s @09:26:59.68 Intake @26.6psia, vacuum from 20.0 to 24.0psi @09:26:59.70 SS.reconfigure forSampling @09:26:59.73 SS.seek 25.00ml @09:27:36.90 ATV.dial :waste @09:27:38.21 AS.seek empty @09:27:39.40 ATV.dial :air3 @09:27:39.97 AS.reconfigure ASslow1 @09:27:40.01 AS.seek 0.009ml @09:27:42.26 delay 2 seconds @09:27:44.27 ATV.dial :bleach @09:27:44.64 AS.seek 0.579ml @09:28:26.83 Stroke completed -- waiting for pressure to equalize ... @09:28:49.81 Intake.select :close @09:28:49.94 SSV.dial :exhaust @09:28:50.72 Exhaust.select :open @09:28:50.82 SS.reconfigure SSexhale @09:28:50.86 SS.seek 0.00ml @09:28:59.56 Exhaust.select :close @09:28:59.66 SSV.dial CBV @09:28:59.68 CBV.dial SSV @09:29:00.47 Allowing 185s for 25.0ml of remaining 750.0ml @09:29:00.48 Intake.select :open @09:29:02.62 Intake @29.1psia, vacuum from 20.0 to 24.0psi @09:29:02.64 SS.reconfigure forSampling @09:29:02.67 SS.seek 25.00ml @09:29:10.52 Vacuum too high -- waiting up to 40s @09:29:11.65 Intake @27.6psia, vacuum from 20.0 to 24.0psi @09:29:11.66 SS.reconfigure forSampling @09:29:11.70 SS.seek 25.00ml @09:29:21.12 Vacuum too high -- waiting up to 40s @09:29:23.30 Intake @26.2psia, vacuum from 19.7 to 23.7psi @09:29:23.31 SS.reconfigure forSampling @09:29:23.35 SS.seek 25.00ml @09:29:40.62 Vacuum too high -- waiting up to 40s @09:29:41.16 delay 3 seconds @09:29:44.88 ATV.dial :air3 @09:29:45.11 Intake @24.9psia, vacuum from 18.4 to 22.4psi @09:29:45.13 SS.seek 25.00ml @09:29:45.24 delay 3 seconds @09:29:48.29 AS.seek 0.604ml @09:29:53.69 delay 3 seconds @09:29:56.70 ATV.dial :bleach @09:29:57.09 AS.seek 0.589ml @09:29:58.56 Vacuum too high -- waiting up to 40s @09:30:00.40 delay 3 seconds @09:30:03.41 ATV.dial :ASV @09:30:04.30 ASV.dial :wasteR @09:30:05.15 Intake @25.0psia, vacuum from 18.5 to 22.5psi @09:30:05.17 SS.seek 25.00ml @09:30:05.25 AS.seek 0.000ml @09:30:23.18 Vacuum too high -- waiting up to 40s @09:30:31.70 Intake @25.2psia, vacuum from 18.7 to 22.7psi @09:30:31.72 SS.seek 25.00ml @09:30:51.62 Vacuum too high -- waiting up to 40s @09:31:00.49 Intake @24.6psia, vacuum from 18.1 to 22.1psi @09:31:00.51 SS.seek 25.00ml @09:31:19.49 Vacuum too high -- waiting up to 40s @09:31:31.30 SS.seek 25.00ml @09:31:37.71 Stroke completed -- waiting for pressure to equalize ... @09:31:57.89 Intake.select :close @09:31:58.03 SSV.dial :exhaust @09:31:58.80 Exhaust.select :open @09:31:58.90 SS.reconfigure SSexhale @09:31:58.94 SS.seek 0.00ml @09:32:05.67 delay 1:00 @09:32:07.75 Exhaust.select :close @09:32:07.85 SSV.dial CBV @09:32:07.87 CBV.dial SSV @09:32:08.66 Allowing 185s for 25.0ml of remaining 725.0ml @09:32:08.67 Intake.select :open @09:32:10.81 Intake @26.1psia, vacuum from 19.6 to 23.6psi @09:32:10.83 SS.reconfigure forSampling @09:32:10.86 SS.seek 25.00ml @09:32:22.84 Vacuum too high -- waiting up to 40s @09:32:26.58 Intake @24.6psia, vacuum from 18.1 to 22.1psi @09:32:26.59 SS.seek 25.00ml @09:32:34.26 Vacuum too high -- waiting up to 40s @09:32:40.66 Intake @24.4psia, vacuum from 17.9 to 21.9psi @09:32:40.68 SS.seek 25.00ml @09:32:51.96 Vacuum too high -- waiting up to 40s @09:32:58.82 Intake @23.9psia, vacuum from 17.4 to 21.4psi @09:32:58.84 SS.seek 25.00ml @09:33:05.68 AFV.select :carrierSolution @09:33:05.77 delay 2 seconds @09:33:07.78 AS.reconfigure ASconfig @09:33:07.81 AS.seek 1.000ml @09:33:09.43 Vacuum too high -- waiting up to 40s @09:33:17.33 Intake @23.2psia, vacuum from 16.7 to 20.7psi @09:33:17.34 SS.seek 25.00ml @09:33:27.45 Vacuum too high -- waiting up to 40s @09:33:36.81 Intake @23.3psia, vacuum from 16.8 to 20.8psi @09:33:36.83 SS.seek 25.00ml @09:33:48.30 Vacuum too high -- waiting up to 40s @09:33:58.51 Intake @23.0psia, vacuum from 16.5 to 20.5psi @09:33:58.53 SS.seek 25.00ml @09:34:06.36 delay 1 second @09:34:07.37 AFV.select :holdingCoil @09:34:07.46 delay 1 second @09:34:08.47 ATV.dial :ASV @09:34:08.48 ASV.dial :wasteR @09:34:08.49 AS.reconfigure ASslow1 @09:34:08.52 AS.seek 0.000ml @09:34:10.83 Vacuum too high -- waiting up to 40s @09:34:24.06 Intake @24.8psia, vacuum from 18.3 to 22.3psi @09:34:24.08 SS.seek 25.00ml @09:34:40.89 Vacuum too high -- waiting up to 40s @09:34:53.10 Intake @24.9psia, vacuum from 18.4 to 22.4psi @09:34:53.11 SS.seek 25.00ml @09:35:08.62 Vacuum too high -- waiting up to 40s @09:35:13.70 SS.reconfigure SSconfig @09:35:13.74 SS.hold @09:35:13.83 Intake.select :close @09:35:13.99 ShallowSampler::Clogged in WCR -- progress too slow after 299.2ml @09:35:14.00 SSV.dial :exhaust @09:35:14.77 Exhaust.select :open @09:35:14.88 SS.reconfigure SSexhale @09:35:14.92 SS.seek 0.00ml @09:35:23.85 Exhaust.select :close @09:35:23.96 Sampled 299.2ml @09:35:23.97 collectSample: Complete @09:35:23.98 Drying puck before fixing for 04:00.00 @09:35:23.99 CBV.dial :waste2b @09:35:24.76 delay 1:00 @09:35:26.95 Sleepy.power :sampler,:OFF @09:36:24.76 CSV.dial :air @09:36:25.21 CS.reconfigure CSfast @09:36:25.24 CS.seek 10.000ml @09:36:49.10 CBV.dial between CSV and waste2b @09:36:49.43 Pumping wcr puck from tube 2 up to 55psi... @09:36:49.48 CBV.dial SSV @09:36:50.92 CBV.dial between CSV and waste2b @09:36:51.87 CSV.dial between air and CTV @09:36:52.27 CS.seek 6.505ml @09:37:01.17 CTV.dial CSV,avoiding => air @09:37:01.19 CSV.dial CTV @09:37:01.94 CS.reconfigure CSpressurize @09:37:01.97 CS.seek 0.000ml @09:37:19.04 CSV.dial :air @09:37:19.56 CS.reconfigure CSfast @09:37:19.59 CS.seek 10.000ml @09:37:32.78 delay 20 seconds @09:37:43.36 CSV.dial between air and CTV @09:37:43.75 CS.seek 2.132ml @09:37:52.79 ATV.dial :air3 @09:37:53.93 AS.seek 0.590ml @09:38:02.55 CTV.dial CSV,avoiding => air @09:38:02.56 CSV.dial CTV @09:38:02.86 CS.reconfigure CSpressurize @09:38:02.89 CS.seek 0.000ml @09:38:04.28 CBV.dial :waste2b @09:38:04.61 CS.reconfigure CSconfig @09:38:04.64 CS.seek empty @09:38:09.85 delay 1:00 @09:39:09.86 CTV.dial :air,avoiding => intake @09:39:11.14 Fix #1 of 2 (with rnal) for 02:00.00 @09:39:11.15 CS Pulling 2ml of rnal CSV.dial CSR @09:39:11.58 CSR.select :rnal @09:39:11.68 CS.seek 2.000ml @09:39:17.63 delay 5 seconds @09:39:22.64 CSR.select :air @09:39:22.73 CS.reconfigure CSfast @09:39:22.77 CS.seek 10.000ml @09:39:41.88 CBV.dial between CSV and waste2b @09:39:42.21 Pumping wcr puck from tube 2 up to 55psi... @09:39:42.25 CBV.dial SSV @09:39:43.68 CBV.dial between CSV and waste2b @09:39:44.63 CSV.dial between air and CTV @09:39:45.92 CS.seek 6.592ml @09:39:54.53 delay 3 seconds @09:39:54.57 CTV.dial CSV,avoiding => air @09:39:54.59 CSV.dial CTV @09:39:55.96 CS.reconfigure CSpressurize @09:39:56.00 CS.seek 0.000ml @09:39:57.54 ATV.dial :ASV @09:39:58.64 ASV.dial :wasteR @09:39:58.70 AS.seek 0.090ml @09:40:12.93 delay 2:00 @09:41:40.93 delay 30 seconds @09:42:10.94 ATV.dial :extSrc @09:42:11.56 delay 3 seconds @09:42:12.94 CBV.dial :waste2b @09:42:13.27 CS.reconfigure CSconfig @09:42:13.30 CS.seek empty @09:42:14.03 delay 1:00 @09:42:14.57 ATV.dial :ElutionWater @09:42:15.53 AS.seek 0.120ml @09:42:21.92 ATV.dial :air3 @09:42:22.39 AS.seek 0.130ml @09:42:24.68 delay 2 seconds @09:42:26.69 ATV.dial :bleach @09:42:27.06 AS.seek 0.230ml @09:42:47.73 delay 3 seconds @09:42:50.74 ATV.dial :air3 @09:42:51.09 delay 3 seconds @09:42:54.15 AS.seek 0.255ml @09:42:59.57 delay 3 seconds @09:43:02.58 ATV.dial :bleach @09:43:02.97 AS.seek 0.240ml @09:43:06.27 delay 3 seconds @09:43:09.28 ATV.dial :ASV @09:43:10.16 ASV.dial :SPEdelivery @09:43:10.48 AFV.select :carrierSolution @09:43:10.58 delay 2 seconds @09:43:12.59 AS.reconfigure ASconfig @09:43:12.62 AS.seek 0.500ml @09:43:14.04 CTV.dial :air,avoiding => intake @09:43:15.32 Fix #2 of 2 (with rnal) for 02:00.00 @09:43:15.33 CS Pulling 2ml of rnal @09:43:15.34 CSV.dial CSR @09:43:15.85 CSR.select :rnal @09:43:15.95 CS.seek 2.000ml @09:43:21.93 delay 5 seconds @09:43:26.94 CSR.select :air @09:43:27.03 CS.reconfigure CSfast @09:43:27.07 CS.seek 10.000ml @09:43:28.04 delay 1 second @09:43:29.05 AFV.select :holdingCoil @09:43:29.19 AS.reconfigure ASslow1 @09:43:29.22 AS.seek 0.170ml @09:43:46.27 CBV.dial between CSV and waste2b @09:43:46.59 Pumping wcr puck from tube 2 up to 55psi... @09:43:46.64 CBV.dial SSV @09:43:48.06 CBV.dial between CSV and waste2b @09:43:49.01 CSV.dial between air and CTV @09:43:49.69 CS.seek 6.513ml @09:43:58.50 CTV.dial CSV,avoiding => air @09:43:58.52 CSV.dial CTV @09:43:59.84 CS.reconfigure CSpressurize @09:43:59.87 CS.seek 0.000ml @09:44:16.51 delay 2:00 @09:44:36.78 SPE.hold 2:46:40,95.0C @09:44:36.80 delay 3:00 @09:46:16.52 CBV.dial :waste2b @09:46:16.85 CS.reconfigure CSconfig @09:46:16.88 CS.seek empty @09:46:17.83 delay 1:00 @09:47:17.84 CTV.dial :air,avoiding => intake @09:47:19.20 Storing wcr puck in Collection Clamp to tube 1 @09:47:19.34 SC.reconfigure SCtight @09:47:19.37 SC.seek 1 @09:47:21.68 SC.reconfigure SCloose @09:47:21.85 Elbow.reconfigure TightElbow @09:47:21.89 Elbow.seek 1 @09:47:23.50 Forearm.reconfigure TightForearm @09:47:23.53 Forearm.seek 1 + 800 counts @09:47:24.59 SE.reconfigure SEpick @09:47:24.64 SE.seek beamBreak @09:47:26.33 SE.configure SEconfig @09:47:26.45 Elbow.reconfigure LimpElbow @09:47:26.49 Forearm.reconfigure LooseForearm @09:47:26.53 Forearm.seek 1 @09:47:26.78 Elbow.hold @09:47:26.84 Elbow.reconfigure TightElbow @09:47:26.92 SE.seek 13.06 Pucks below Top Plate @09:47:27.27 Forearm.coast @09:47:27.88 Forearm.seek 1 + 348 counts @09:47:28.13 Forearm.reconfigure TightForearm @09:47:28.17 Forearm.seek 1 + 800 counts @09:47:28.44 SE.seek 14.06 Pucks below Top Plate @09:47:29.00 Forearm.reconfigure LooseForearm @09:47:29.04 Forearm.seek retract @09:47:29.99 Elbow.seek CC @09:47:31.66 Elbow.reconfigure LooseElbow @09:47:31.74 Forearm.seek CC @09:47:32.69 CC.seek sealed @09:47:33.64 Hand.grip @09:47:34.32 CC.seek open @09:47:36.81 AFV.select :carrierSolution @09:47:36.90 delay 2 seconds @09:47:37.72 Forearm.seek retract @09:47:38.71 Elbow.reconfigure TightElbow @09:47:38.74 Elbow.seek 1 @09:47:38.91 AS.reconfigure ASconfig @09:47:38.95 AS.seek 1.000ml @09:47:40.34 Elbow.reconfigure LimpElbow @09:47:40.40 Forearm.seek 1 @09:47:41.46 Elbow.hold @09:47:41.51 Elbow.reconfigure TightElbow @09:47:41.55 Hand.release @09:47:42.40 Forearm.coast @09:47:42.44 Forearm.seek 1 + 317 counts @09:47:42.67 Forearm.reconfigure TightForearm @09:47:42.71 Forearm.seek 1 + 800 counts @09:47:43.06 SE.seek down @09:47:44.17 Forearm.reconfigure LooseForearm @09:47:44.20 Forearm.seek retract @09:47:44.43 SE.coast @09:47:45.11 SC.coast @09:47:45.15 Starting finalPurge Loading FlushPuck into Collection Clamp from FlushPuck::Garage @09:47:45.31 Elbow.seek FlushPuck::Garage @09:47:47.00 Elbow.reconfigure LooseElbow @09:47:47.08 Forearm.seek garage @09:47:48.12 Hand.grip @09:47:48.91 Forearm.seek retract @09:47:49.93 Elbow.reconfigure TightElbow @09:47:49.96 Elbow.seek CC @09:47:52.72 Elbow.reconfigure LooseElbow @09:47:52.80 Forearm.reconfigure TightForearm @09:47:52.83 Forearm.seek CC @09:47:53.97 CC.seek closed @09:47:57.67 Collection Clamp onSpring/offSpring for 5/22 samples, on/off torque 2.15 @09:47:57.72 Hand.release @09:47:58.48 Forearm.reconfigure LooseForearm @09:47:58.51 Forearm.seek retract @09:47:59.43 Flushing the Collection Valve::Manifold @09:47:59.53 CS Pulling 5ml of flush CSV.dial CSR @09:48:00.62 CSR.select :flush @09:48:00.73 CS.seek 5.000ml @09:48:14.29 delay 5 seconds @09:48:19.30 CSR.select :air @09:48:19.44 CS.reconfigure CSfast @09:48:19.47 CS.seek 7.500ml @09:48:26.14 CSV.dial :waste @09:48:27.44 CS.reconfigure CSconfig @09:48:27.47 CS.seek 0.000ml @09:48:27.61 delay 1 second @09:48:28.62 AFV.select :holdingCoil @09:48:28.71 delay 1 second @09:48:29.72 ASV.dial :wasteD @09:48:30.74 AS.seek 0.000ml @09:48:47.29
Flushing lower manifold's waste1 port @09:48:47.39 CSV.dial :flush @09:48:47.85 CS.reconfigure CSfast @09:48:47.88 CS.seek 5.000ml @09:49:00.35 delay 5 seconds @09:49:05.36 CSV.dial :air @09:49:05.86 CS.seek 7.000ml @09:49:11.42 CSV.dial CTV @09:49:11.43
CTV.dial CSV @09:49:12.79 CBV.dial :waste1 @09:49:13.55 CS.seek 0.000ml @09:49:29.31 Priming Eluate @09:49:29.32 AFV.select :carrierSolution @09:49:29.41 delay 2 seconds @09:49:30.35
Flushing lower manifold's waste2b port @09:49:30.46 CSV.dial :flush @09:49:31.27 CS.seek 5.000ml @09:49:31.42 AS.seek 0.500ml @09:49:43.54
delay 5 seconds @09:49:48.55 CSV.dial :air @09:49:49.13 CS.seek 7.000ml @09:49:54.63 CSV.dial CTV @09:49:54.65
CTV.dial CSV @09:49:55.31 CBV.dial :waste2b @09:49:56.06 CS.seek 0.000ml @09:50:00.84 delay 1 second @09:50:01.85 AFV.select :holdingCoil @09:50:01.94 ATV.dial ASV @09:50:01.95 ASV.dial :SPErecovery @09:50:02.89 Swish #1 of 2 @09:50:02.94 AS.reconfigure ASslow0 @09:50:02.98 AS.seek 0.600ml @09:50:12.91
Flushing upper manifold @09:50:13.00 CSV.dial :flush @09:50:13.47 AS.seek 0.500ml @09:50:13.73
CS.seek 5.000ml @09:50:23.93 Swish #2 of 2 @09:50:23.99 AS.seek 0.600ml @09:50:25.97
delay 5 seconds @09:50:30.98 CSV.dial :air @09:50:31.54 CS.seek 7.000ml @09:50:34.53 AS.seek 0.500ml @09:50:37.08 CSV.dial CBV @09:50:37.09
CBV.dial CSV @09:50:38.25 CTV.dial :air @09:50:39.53 CS.seek 0.000ml @09:50:45.03 delay 3 seconds @09:50:48.09 AS.reconfigure ASslow1 @09:50:48.12 AS.seek 0.676ml @09:50:56.38
delay 7 seconds @09:51:03.39 CS.seek 3.000ml @09:51:11.13 CSV.dial :waste @09:51:11.65 CS.seek 0.000ml @09:51:19.44 CSV.dial :flush @09:51:19.87 CS.seek 3.000ml @09:51:24.29 delay 3 seconds @09:51:27.30 Eluate primed @09:51:27.31 ATV.dial :waste2 @09:51:27.66
delay 5 seconds @09:51:27.83 AS.reconfigure ASnorm @09:51:27.86 AS.seek 0.000ml @09:51:32.68
CSV.dial :air @09:51:33.17 CS.seek 5.000ml @09:51:38.54 CSV.dial :air @09:51:38.56 CS.seek 0.000ml @09:51:51.07 delay 7 seconds @09:51:58.08 CS.seek 3.000ml @09:52:02.75 SPE.coast @09:52:02.83 ASV.dial :SPErecovery @09:52:02.84 Setting up PCR for HF183em -- writing /var/log/esp/HF183em14feb1709h1ml.pcr @09:52:02.85 Building PCR Slug from ASV with MM2 mix, PP4 primer @09:52:02.86 Priming with carrier @09:52:02.87 ATV.dial ARV @09:52:03.57 ARV.dial :carrier @09:52:03.87 AS.seek 0.500ml @09:52:05.79
CSV.dial :waste @09:52:06.60 CS.seek 0.000ml @09:52:14.20 Disinfecting external Intake with mfbkill @09:52:14.29 CSV.dial CSR @09:52:15.54 CSR.select :mfbkill @09:52:15.64 CS.seek 8.000ml @09:52:29.74 AFV.select :carrierSolution @09:52:29.84 delay 2 seconds @09:52:31.85 AS.reconfigure ASconfig @09:52:31.89 AS.seek 1.000ml @09:52:35.13
delay 5 seconds @09:52:40.14 CSR.select :air @09:52:40.23 CS.seek 10.000ml @09:52:45.57 CSV.dial CBV @09:52:45.59
CBV.dial CSV @09:52:46.65 CTV.dial :intake @09:52:47.35 Intake.select :open @09:52:47.45 CS.seek 2.000ml @09:53:01.36 delay 1 second @09:53:02.37 AFV.select :holdingCoil @09:53:02.47 delay 1 second @09:53:03.48 ATV.dial :waste @09:53:04.76 AS.reconfigure ASnorm @09:53:04.79 AS.seek empty @09:53:06.51
Intake.select :close @09:53:06.60 CTV.dial :air @09:53:07.36 CS.reconfigure CSconfig @09:53:07.39 CS.seek 0.000ml @09:53:13.29 Flushing lower manifold's waste1 port @09:53:13.30 CS Pulling 4ml of mfbkill @09:53:13.31 CSV.dial CSR @09:53:14.35 CSR.select :mfbkill @09:53:14.45 CS.seek 4.000ml @09:53:25.32 delay 5 seconds @09:53:30.33 CSR.select :air @09:53:30.42 CS.reconfigure CSfast @09:53:30.46 CS.seek 10.000ml @09:53:44.92 CSV.dial CTV @09:53:44.94
CTV.dial CSV @09:53:46.27 CBV.dial :waste1 @09:53:46.72 CS.seek 0.000ml @09:53:56.25 AFV.select :carrierSolution @09:53:56.36 delay 2 seconds @09:53:58.37 AS.reconfigure ASconfig @09:53:58.40 AS.seek 0.500ml @09:54:10.07
Flushing lower manifold's waste2b port @09:54:10.08 CS Pulling 4ml of mfbkill @09:54:10.09 CSV.dial CSR @09:54:10.58 CSR.select :mfbkill @09:54:10.68 CS.reconfigure CSconfig @09:54:10.72 CS.seek 4.000ml @09:54:21.63 delay 5 seconds @09:54:26.64 CSR.select :air @09:54:26.73 CS.reconfigure CSfast @09:54:26.77 CS.seek 10.000ml @09:54:27.87 delay 1 second @09:54:28.88 AFV.select :holdingCoil @09:54:28.98 Building Slug @09:54:28.99 ATV.dial :ARV @09:54:30.25 delay 2 seconds @09:54:32.26 ARV.dial :air1 @09:54:32.59 delay 2 seconds @09:54:34.65 AS.reconfigure ASslow2 @09:54:34.68 AS.seek 0.506ml @09:54:41.05 CSV.dial CTV @09:54:41.07
CTV.dial CSV @09:54:41.14 delay 2 seconds @09:54:41.58
CBV.dial :waste2b @09:54:42.33 CS.seek 0.000ml @09:54:43.15 ARV.dial :MM2 @09:54:44.40 delay 2 seconds @09:54:46.40 Adding MM2 @09:54:46.46 AS.seek 0.524ml @09:55:05.23 delay 25 seconds @09:55:05.63
Killing upper manifold @09:55:05.64 CS Pulling 4ml of mfbkill @09:55:05.65 CSV.dial CSR @09:55:06.14 CSR.select :mfbkill @09:55:06.24 CS.reconfigure CSconfig @09:55:06.28 CS.seek 4.000ml @09:55:17.25 delay 5 seconds @09:55:22.26 CSR.select :air @09:55:22.35 CS.reconfigure CSfast @09:55:22.39 CS.seek 10.000ml @09:55:30.24 ARV.dial :PP4 @09:55:30.58 Adding PP4 @09:55:30.59 delay 2 seconds @09:55:32.64 AS.seek 0.530ml @09:55:36.72 CSV.dial CBV @09:55:36.74
CBV.dial CSV @09:55:38.16 CTV.dial :air @09:55:39.14 delay 10 seconds @09:55:39.43
CS.seek 0.000ml @09:55:49.15 ARV.dial :air3 @09:55:49.48 adding ARV air @09:55:49.49 delay 2 seconds @09:55:51.55 AS.seek 0.536ml @09:55:58.01 delay 5 seconds @09:56:02.72
delay 7 seconds @09:56:03.02 ARV.dial :carrier @09:56:04.15 delay 7 seconds @09:56:09.73
CS.seek 3.000ml @09:56:11.21 AS.seek 0.594ml @09:56:17.41
CSV.dial :waste @09:56:17.85 CS.seek 0.000ml @09:56:25.53 Flushing upper manifold @09:56:25.62 CSV.dial :flush @09:56:26.06 CS.seek 5.000ml @09:56:38.16 delay 5 seconds @09:56:43.17 CSV.dial :air @09:56:43.64 CS.seek 6.999ml @09:56:48.97 CSV.dial CBV @09:56:48.99
CBV.dial CSV @09:56:50.62 CTV.dial :air @09:56:50.63 CS.seek 0.000ml @09:57:07.06 delay 7 seconds @09:57:10.74 delay 5 seconds @09:57:14.07
CS.seek 3.000ml @09:57:15.75 ATV.dial :ASV @09:57:16.10 delay 2 seconds @09:57:18.15 AS.seek 0.600ml @09:57:21.62
CSV.dial :waste @09:57:22.13 CS.seek 0.000ml @09:57:24.67 delay 4 seconds @09:57:28.68 ATV.dial ARV @09:57:29.12 delay 2 seconds @09:57:29.84
CSV.dial :flush @09:57:30.27 CS.seek 3.000ml @09:57:31.13 ARV.dial :carrier @09:57:31.14 delay 2 seconds @09:57:33.20 AS.seek 0.650ml @09:57:37.90
delay 5 seconds @09:57:42.91 CSV.dial :air @09:57:43.37 CS.seek 5.000ml @09:57:48.71 CSV.dial :air @09:57:48.73 CS.seek 0.000ml @09:58:00.65 delay 7 seconds @09:58:07.66 CS.seek 3.000ml @09:58:15.21 CSV.dial :waste @09:58:16.01 CS.seek 0.000ml @09:58:23.53 Flushing lower manifold's waste1 port @09:58:23.62 CSV.dial :flush @09:58:24.13 CS.seek 5.000ml @09:58:24.49 delay 4 seconds @09:58:28.50 ATV.dial PCR @09:58:29.02 ASV.dial :waste1 @09:58:29.87 ARV.dial :air1 @09:58:30.22 Positioning Slug by 0.324ml @09:58:30.27 AS.seek 0.326ml @09:58:36.15
delay 5 seconds @09:58:41.16 CSV.dial :air @09:58:41.64 CS.seek 7.000ml @09:58:46.96 CSV.dial CTV @09:58:46.98
CTV.dial CSV @09:58:48.34 CBV.dial :waste1 @09:58:48.78 CS.seek 0.000ml @09:59:05.40 Flushing lower manifold's waste2b port @09:59:05.49 CSV.dial :flush @09:59:06.30 CS.seek 5.000ml @09:59:18.30 delay 5 seconds @09:59:23.31 CSV.dial :air @09:59:23.87 CS.seek 7.000ml @09:59:29.21 CSV.dial CTV @09:59:29.23
CTV.dial CSV @09:59:29.66 CBV.dial :waste2b @09:59:30.39 CS.seek 0.000ml @09:59:46.77 Disinfecting external Intake with kill @09:59:46.86 CSV.dial CSR @09:59:47.29 CSR.select :kill @09:59:47.39 CS.seek 8.000ml @10:00:06.15 delay 5 seconds @10:00:11.16 CSR.select :air @10:00:11.25 CS.seek 10.000ml @10:00:16.58 CSV.dial CBV @10:00:16.60
CBV.dial CSV @10:00:17.63 CTV.dial :intake @10:00:18.36 Intake.select :open @10:00:18.45 CS.seek 2.000ml @10:00:37.40 Intake.select :close @10:00:37.49 CTV.dial :air @10:00:38.21 CS.reconfigure CSconfig @10:00:38.25 CS.seek 0.000ml @10:00:44.11 CSV.dial CSR @10:00:45.38 CSR.select :flush @10:00:45.48 CS.seek 5.000ml @10:00:58.93 delay 5 seconds @10:01:03.94 CSR.select :air @10:01:04.03 CS.seek 10.000ml @10:01:17.55 CSV.dial CBV @10:01:18.53 CS.seek 0.000ml @10:01:44.64 Garaging FlushPuck in Collection Clamp @10:01:44.70 Elbow.reconfigure TightElbow @10:01:44.73 Elbow.seek CC @10:01:44.83 Elbow.reconfigure LooseElbow @10:01:44.91 Forearm.seek CC @10:01:45.87 CC.seek sealed @10:01:46.80 Hand.grip @10:01:47.54 CC.seek open @10:01:50.84 Forearm.seek retract @10:01:51.79 Elbow.reconfigure TightElbow @10:01:51.82 Elbow.seek garage @10:01:54.63 Elbow.reconfigure LooseElbow @10:01:54.71 Forearm.reconfigure TightForearm @10:01:54.74 Forearm.seek garage @10:01:55.89 Hand.release @10:01:56.71 Forearm.reconfigure LooseForearm @10:01:56.74 Forearm.seek retract @10:01:57.77 Completed {wqPCR} begun 08:01:34 @10:01:57.80 deferred until MFBPCR finish @10:01:57.81
Waiting for MFBPCR til 08:01PST18-Feb-14 @10:01:57.82 Sleeping until 08:01PST18-Feb-14 @10:01:57.83 GoodNews.email "Then, in tube 2: wqPCR\n/home/esp/esp2local/mission/14feb14sccwrp.rb:63",Subject => "Waiting until 08:01PST18-Feb-14" @10:04:00.90 delay 5 seconds @10:04:05.91 ATV.dial ARV @10:04:06.42 ASV.dial :PCRlock @10:04:06.75 delay 5 seconds @10:04:11.76 Running HF183em ProfileEM60C2 thermocycling @10:04:11.77 PCR.program ProfileEM60C2 @10:04:11.88 ProfileEM60C2 should complete in < 2:11:38 @10:04:11.89 PCR.run 2:49:32.5 @11:57:30.42 PCR.off @11:57:30.95 PCR for HF183em completed -- closed /var/log/esp/HF183em14feb1709h1ml.pcr @11:57:31.00 Cleaning PCR @11:57:31.01 AFV.select :carrierSolution @11:57:31.10 delay 2 seconds @11:57:33.11 AS.reconfigure ASconfig @11:57:33.14 AS.seek 0.500ml @11:57:43.56 delay 1 second @11:57:44.57 AFV.select :holdingCoil @11:57:44.66 ASV.dial :waste1 @11:57:45.14 ATV.dial :air3 @11:57:46.10 AS.reconfigure ASslow1 @11:57:46.13 AS.seek 0.510ml @11:57:48.41 delay 2 seconds @11:57:50.42 ATV.dial :bleach @11:57:50.82 AS.seek 0.810ml @11:58:52.25 delay 3 seconds @11:58:55.26 ATV.dial :air3 @11:58:55.61 delay 3 seconds @11:58:58.67 AS.seek 0.835ml @11:59:04.00 delay 3 seconds @11:59:07.01 ATV.dial :bleach @11:59:07.41 AS.seek 0.820ml @11:59:10.72 delay 3 seconds @11:59:13.73 ARV.dial :waste @11:59:14.33 delay 3 seconds @11:59:17.34 ATV.dial :ARV @11:59:18.17 AS.reconfigure ASconfig @11:59:18.20 AS.seek 0.470ml @11:59:38.89 AFV.select :carrierSolution @11:59:38.99 delay 2 seconds @11:59:41.00 AS.seek 1.000ml @12:00:12.18 delay 1 second @12:00:13.19 AFV.select :holdingCoil @12:00:13.28 delay 1 second @12:00:14.29 AS.reconfigure ASfast @12:00:14.32 AS.seek 0.000ml @12:00:42.06 AFV.select :carrierSolution @12:00:42.16 delay 2 seconds @12:00:44.17 AS.reconfigure ASconfig @12:00:44.20 AS.seek 1.000ml @12:01:42.79 delay 1 second @12:01:43.80 AFV.select :holdingCoil @12:01:43.89 delay 1 second @12:01:44.90 AS.reconfigure ASfast @12:01:44.93 AS.seek 0.000ml @12:02:12.65 AFV.select :carrierSolution @12:02:12.75 delay 2 seconds @12:02:14.76 AS.reconfigure ASconfig @12:02:14.80 AS.seek 1.000ml @12:03:13.43 delay 1 second @12:03:14.44 AFV.select :holdingCoil @12:03:14.53 delay 1 second @12:03:15.54 AS.reconfigure ASfast @12:03:15.57 AS.seek 0.000ml @12:03:43.30 ARV.dial between carrier and air1 @12:03:43.82 ATV.dial :air3 @12:03:44.77 AS.reconfigure ASslow1 @12:03:44.80 AS.seek 0.010ml @12:03:47.11 delay 2 seconds @12:03:49.12 ATV.dial :bleach @12:03:49.49 AS.seek 0.810ml @12:06:33.05 delay 3 seconds @12:06:36.06 ATV.dial :air3 @12:06:36.48 delay 3 seconds @12:06:39.54 AS.seek 0.835ml @12:06:44.89 delay 3 seconds @12:06:47.90 ATV.dial :bleach @12:06:48.39 AS.seek 0.820ml @12:06:51.72 delay 3 seconds @12:06:54.73 ATV.dial PCR @12:06:55.09 AS.reconfigure ASconfig @12:06:55.12 AS.seek empty @12:07:43.25 PCR.program :bleachProfile @12:07:43.31 PCR program should complete in < 2:45 @12:07:43.32 PCR.run 10:00 @12:10:44.59 PCR.off @12:10:44.62 AFV.select :carrierSolution @12:10:44.71 delay 2 seconds @12:10:46.72 AS.seek 1.000ml @12:11:45.39 delay 1 second @12:11:46.40 AFV.select :holdingCoil @12:11:46.49 delay 1 second @12:11:47.50 AS.reconfigure ASfast @12:11:47.53 AS.seek 0.000ml @12:12:15.27 delay 3 seconds @12:12:18.28 AFV.select :carrierSolution @12:12:18.37 delay 2 seconds @12:12:20.38 AS.reconfigure ASconfig @12:12:20.41 AS.seek 1.000ml @12:13:19.01 delay 1 second @12:13:20.02 AFV.select :holdingCoil @12:13:20.11 delay 1 second @12:13:21.12 AS.reconfigure ASfast @12:13:21.15 AS.seek 0.000ml @12:13:48.87 delay 3 seconds @12:13:51.88 AFV.select :carrierSolution @12:13:51.97 delay 2 seconds @12:13:53.98 AS.reconfigure ASconfig @12:13:54.01 AS.seek 1.000ml @12:14:52.60 delay 1 second @12:14:53.61 AFV.select :holdingCoil @12:14:53.70 delay 1 second @12:14:54.71 AS.reconfigure ASfast @12:14:54.74 AS.seek 0.000ml @12:15:22.46 delay 3 seconds @12:15:25.47 AFV.select :carrierSolution @12:15:25.56 delay 2 seconds @12:15:27.57 AS.reconfigure ASconfig @12:15:27.60 AS.seek 1.000ml @12:16:26.18 delay 1 second @12:16:27.19 AFV.select :holdingCoil @12:16:27.28 delay 1 second @12:16:28.29 AS.reconfigure ASfast @12:16:28.85 AS.seek 0.000ml @12:16:56.59 delay 3 seconds @12:16:59.60 AFV.select :carrierSolution @12:16:59.69 delay 2 seconds @12:17:01.70 AS.reconfigure ASconfig @12:17:01.73 AS.seek 1.000ml @12:18:00.35 delay 1 second @12:18:01.36 AFV.select :holdingCoil @12:18:01.46 delay 1 second @12:18:02.47 AS.reconfigure ASnorm @12:18:02.51 AS.seek 0.000ml @12:18:53.99 Setting up PCR for ENTap -- writing /var/log/esp/ENTap14feb1712h1ml.pcr @12:18:54.00 Building PCR Slug from ASV with MM1 mix, PP1 primer @12:18:54.01 Priming with carrier @12:18:54.02 ATV.dial ARV @12:18:54.53 ARV.dial :carrier @12:18:54.80 AS.seek 0.500ml @12:19:20.67 AFV.select :carrierSolution @12:19:20.77 delay 2 seconds @12:19:22.78 AS.reconfigure ASconfig @12:19:22.82 AS.seek 1.000ml @12:19:52.25 delay 1 second @12:19:53.26 AFV.select :holdingCoil @12:19:53.36 delay 1 second @12:19:54.37 ATV.dial :waste @12:19:55.77 AS.reconfigure ASnorm @12:19:55.81 AS.seek empty @12:20:47.25 AFV.select :carrierSolution @12:20:47.35 delay 2 seconds @12:20:49.36 AS.reconfigure ASconfig @12:20:49.39 AS.seek 0.500ml @12:21:18.91 delay 1 second @12:21:19.92 AFV.select :holdingCoil @12:21:20.02 Building Slug @12:21:20.03 ATV.dial :ARV @12:21:21.30 delay 2 seconds @12:21:23.31 ARV.dial :air1 @12:21:23.64 delay 2 seconds @12:21:25.70 AS.reconfigure ASslow2 @12:21:25.73 AS.seek 0.506ml @12:21:32.05 delay 2 seconds @12:21:34.06 ARV.dial :MM1 @12:21:34.56 delay 2 seconds @12:21:36.57 Adding MM1 @12:21:36.63 AS.seek 0.524ml @12:21:55.20 delay 25 seconds @12:22:20.21 ARV.dial :PP1 @12:22:20.55 Adding PP1 @12:22:20.56 delay 2 seconds @12:22:22.61 AS.seek 0.530ml @12:22:29.12 delay 10 seconds @12:22:39.13 ARV.dial :air1 @12:22:39.44 adding ARV air @12:22:39.45 delay 2 seconds @12:22:41.50 AS.seek 0.536ml @12:22:48.11 delay 5 seconds @12:22:53.12 ARV.dial :carrier @12:22:53.43 delay 7 seconds @12:23:00.49 AS.seek 0.594ml @12:23:59.90 delay 5 seconds @12:24:04.91 ATV.dial :ASV @12:24:05.32 delay 2 seconds @12:24:07.38 AS.seek 0.600ml @12:24:13.81 delay 4 seconds @12:24:17.82 ATV.dial ARV @12:24:18.15 delay 2 seconds @12:24:20.16 ARV.dial :carrier @12:24:20.17 delay 2 seconds @12:24:22.22 AS.seek 0.650ml @12:25:13.55 delay 4 seconds @12:25:17.56 ATV.dial PCR @12:25:18.16 ASV.dial :waste1 @12:25:18.17 ARV.dial :air1 @12:25:18.51 Positioning Slug by 0.324ml @12:25:18.57 AS.seek 0.326ml @12:30:49.40 delay 5 seconds @12:30:54.41 ATV.dial ARV @12:30:54.93 ASV.dial :PCRlock @12:30:55.41 delay 5 seconds @12:31:00.42 Running ENTap Profile60C thermocycling @12:31:00.43 PCR.program Profile60C @12:31:00.54 Profile60C should complete in < 1:39:08 @12:31:00.55 PCR.run 2:08:55 @13:52:15.02 PCR.off @13:52:15.54 PCR for ENTap completed -- closed /var/log/esp/ENTap14feb1712h1ml.pcr @13:52:15.59 Cleaning PCR @13:52:15.60 AFV.select :carrierSolution @13:52:15.69 delay 2 seconds @13:52:17.70 AS.reconfigure ASconfig @13:52:17.73 AS.seek 0.500ml @13:52:28.16 delay 1 second @13:52:29.17 AFV.select :holdingCoil @13:52:29.26 ASV.dial :waste1 @13:52:29.75 ATV.dial :air3 @13:52:30.78 AS.reconfigure ASslow1 @13:52:30.81 AS.seek 0.510ml @13:52:33.09 delay 2 seconds @13:52:35.10 ATV.dial :bleach @13:52:35.47 AS.seek 0.810ml @13:53:36.92 delay 3 seconds @13:53:39.93 ATV.dial :air3 @13:53:40.26 delay 3 seconds @13:53:43.32 AS.seek 0.835ml @13:53:48.67 delay 3 seconds @13:53:51.68 ATV.dial :bleach @13:53:52.06 AS.seek 0.820ml @13:53:55.37 delay 3 seconds @13:53:58.38 ARV.dial :waste @13:53:59.00 delay 3 seconds @13:54:02.01 ATV.dial :ARV @13:54:02.77 AS.reconfigure ASconfig @13:54:02.80 AS.seek 0.470ml @13:54:23.50 AFV.select :carrierSolution @13:54:23.59 delay 2 seconds @13:54:25.60 AS.seek 1.000ml @13:54:56.81 delay 1 second @13:54:57.82 AFV.select :holdingCoil @13:54:57.91 delay 1 second @13:54:58.92 AS.reconfigure ASfast @13:54:58.95 AS.seek 0.000ml @13:55:26.68 AFV.select :carrierSolution @13:55:26.78 delay 2 seconds @13:55:28.79 AS.reconfigure ASconfig @13:55:28.82 AS.seek 1.000ml @13:56:27.37 delay 1 second @13:56:28.38 AFV.select :holdingCoil @13:56:28.47 delay 1 second @13:56:29.48 AS.reconfigure ASfast @13:56:29.51 AS.seek 0.000ml @13:56:57.22 AFV.select :carrierSolution @13:56:57.32 delay 2 seconds @13:56:59.33 AS.reconfigure ASconfig @13:56:59.36 AS.seek 1.000ml @13:57:57.97 delay 1 second @13:57:58.98 AFV.select :holdingCoil @13:57:59.07 delay 1 second @13:58:00.08 AS.reconfigure ASfast @13:58:00.11 AS.seek 0.000ml @13:58:27.85 ARV.dial between carrier and air1 @13:58:28.35 ATV.dial :air3 @13:58:29.40 AS.reconfigure ASslow1 @13:58:29.43 AS.seek 0.010ml @13:58:31.75 delay 2 seconds @13:58:33.76 ATV.dial :bleach @13:58:34.13 AS.seek 0.810ml @14:01:17.54 delay 3 seconds @14:01:20.55 ATV.dial :air3 @14:01:20.87 delay 3 seconds @14:01:23.93 AS.seek 0.835ml @14:01:29.28 delay 3 seconds @14:01:32.29 ATV.dial :bleach @14:01:32.67 AS.seek 0.820ml @14:01:35.98 delay 3 seconds @14:01:38.99 ATV.dial PCR @14:01:39.32 AS.reconfigure ASconfig @14:01:39.35 AS.seek empty @14:02:27.48 PCR.program :bleachProfile @14:02:27.54 PCR program should complete in < 2:45 @14:02:27.55 PCR.run 10:00 @14:05:28.76 PCR.off @14:05:28.79 AFV.select :carrierSolution @14:05:28.88 delay 2 seconds @14:05:31.45 AS.seek 1.000ml @14:06:30.11 delay 1 second @14:06:31.12 AFV.select :holdingCoil @14:06:31.21 delay 1 second @14:06:32.22 AS.reconfigure ASfast @14:06:32.26 AS.seek 0.000ml @14:07:00.00 delay 3 seconds @14:07:03.01 AFV.select :carrierSolution @14:07:03.10 delay 2 seconds @14:07:05.11 AS.reconfigure ASconfig @14:07:05.15 AS.seek 1.000ml @14:08:03.75 delay 1 second @14:08:04.76 AFV.select :holdingCoil @14:08:04.85 delay 1 second @14:08:05.86 AS.reconfigure ASfast @14:08:05.90 AS.seek 0.000ml @14:08:33.60 delay 3 seconds @14:08:36.61 AFV.select :carrierSolution @14:08:36.70 delay 2 seconds @14:08:38.71 AS.reconfigure ASconfig @14:08:38.74 AS.seek 1.000ml @14:09:37.27 delay 1 second @14:09:38.28 AFV.select :holdingCoil @14:09:38.37 delay 1 second @14:09:39.38 AS.reconfigure ASfast @14:09:39.41 AS.seek 0.000ml @14:10:07.12 delay 3 seconds @14:10:10.13 AFV.select :carrierSolution @14:10:10.22 delay 2 seconds @14:10:12.23 AS.reconfigure ASconfig @14:10:12.26 AS.seek 1.000ml @14:11:10.88 delay 1 second @14:11:11.89 AFV.select :holdingCoil @14:11:11.98 delay 1 second @14:11:12.99 AS.reconfigure ASfast @14:11:13.02 AS.seek 0.000ml @14:11:40.75 delay 3 seconds @14:11:43.76 AFV.select :carrierSolution @14:11:43.85 delay 2 seconds @14:11:45.86 AS.reconfigure ASconfig @14:11:45.89 AS.seek 1.000ml @14:12:44.52 delay 1 second @14:12:45.53 AFV.select :holdingCoil @14:12:45.62 delay 1 second @14:12:46.63 AS.reconfigure ASnorm @14:12:46.66 AS.seek 0.000ml @14:13:38.11 Setting up PCR for ENTem -- writing /var/log/esp/ENTem14feb1714h1ml.pcr @14:13:38.12 Building PCR Slug from ASV with MM2 mix, PP3 primer @14:13:38.13 Priming with carrier @14:13:38.14 ATV.dial ARV @14:13:38.66 ARV.dial :carrier @14:13:38.96 AS.seek 0.500ml @14:14:04.84 AFV.select :carrierSolution @14:14:04.95 delay 2 seconds @14:14:06.96 AS.reconfigure ASconfig @14:14:07.00 AS.seek 1.000ml @14:14:36.40 delay 1 second @14:14:37.41 AFV.select :holdingCoil @14:14:37.51 delay 1 second @14:14:38.52 ATV.dial :waste @14:14:39.79 AS.reconfigure ASnorm @14:14:39.82 AS.seek empty @14:15:31.29 AFV.select :carrierSolution @14:15:31.39 delay 2 seconds @14:15:33.40 AS.reconfigure ASconfig @14:15:33.44 AS.seek 0.500ml @14:16:02.96 delay 1 second @14:16:03.97 AFV.select :holdingCoil @14:16:04.07 Building Slug @14:16:04.08 ATV.dial :ARV @14:16:05.43 delay 2 seconds @14:16:07.44 ARV.dial :air1 @14:16:07.76 delay 2 seconds @14:16:09.82 AS.reconfigure ASslow2 @14:16:09.85 AS.seek 0.506ml @14:16:16.31 delay 2 seconds @14:16:18.32 ARV.dial :MM2 @14:16:19.65 delay 2 seconds @14:16:21.66 Adding MM2 @14:16:21.72 AS.seek 0.524ml @14:16:40.39 delay 25 seconds @14:17:05.40 ARV.dial :PP3 @14:17:05.72 Adding PP3 @14:17:05.73 delay 2 seconds @14:17:07.78 AS.seek 0.530ml @14:17:14.01 delay 10 seconds @14:17:24.02 ARV.dial :air2 @14:17:24.36 adding ARV air @14:17:24.37 delay 2 seconds @14:17:26.42 AS.seek 0.536ml @14:17:32.73 delay 5 seconds @14:17:37.74 ARV.dial :carrier @14:17:38.77 delay 7 seconds @14:17:45.83 AS.seek 0.594ml @14:18:45.12 delay 5 seconds @14:18:50.13 ATV.dial :ASV @14:18:50.47 delay 2 seconds @14:18:52.53 AS.seek 0.600ml @14:18:58.96 delay 4 seconds @14:19:02.97 ATV.dial ARV @14:19:03.41 delay 2 seconds @14:19:05.42 ARV.dial :carrier @14:19:05.43 delay 2 seconds @14:19:07.48 AS.seek 0.650ml @14:19:58.80 delay 4 seconds @14:20:02.81 ATV.dial PCR @14:20:03.33 ASV.dial :waste1 @14:20:03.34 ARV.dial :air1 @14:20:03.69 Positioning Slug by 0.324ml @14:20:03.74 AS.seek 0.326ml @14:25:34.32 delay 5 seconds @14:25:39.33 ATV.dial ARV @14:25:39.85 ASV.dial :PCRlock @14:25:40.35 delay 5 seconds @14:25:45.36 Running ENTem ProfileEM60C thermocycling @14:25:45.37 PCR.program ProfileEM60C @14:25:45.48 ProfileEM60C should complete in < 2:11:38 @14:25:45.49 PCR.run 2:49:32.5 @16:19:53.62 PCR.off @16:19:54.15 PCR for ENTem completed -- closed /var/log/esp/ENTem14feb1714h1ml.pcr @16:19:54.21 Cleaning PCR AFV.select :carrierSolution @16:19:54.31 delay 2 seconds @16:19:56.32 AS.reconfigure ASconfig @16:19:56.35 AS.seek 0.500ml @16:20:06.77 delay 1 second @16:20:07.78 AFV.select :holdingCoil @16:20:07.87 ASV.dial :waste1 @16:20:08.35 ATV.dial :air3 @16:20:09.38 AS.reconfigure ASslow1 @16:20:09.41 AS.seek 0.510ml @16:20:11.69 delay 2 seconds @16:20:13.70 ATV.dial :bleach @16:20:14.07 AS.seek 0.810ml @16:21:15.50 delay 3 seconds @16:21:18.51 ATV.dial :air3 @16:21:18.83 delay 3 seconds @16:21:21.89 AS.seek 0.835ml @16:21:27.22 delay 3 seconds @16:21:30.23 ATV.dial :bleach @16:21:30.62 AS.seek 0.820ml @16:21:33.97 delay 3 seconds @16:21:36.98 ARV.dial :waste @16:21:37.58 delay 3 seconds @16:21:40.59 ATV.dial :ARV @16:21:41.33 AS.reconfigure ASconfig @16:21:41.36 AS.seek 0.470ml @16:22:02.05 AFV.select :carrierSolution @16:22:02.14 delay 2 seconds @16:22:04.15 AS.seek 1.000ml @16:22:35.39 delay 1 second @16:22:36.40 AFV.select :holdingCoil @16:22:36.49 delay 1 second @16:22:37.50 AS.reconfigure ASfast @16:22:37.53 AS.seek 0.000ml @16:23:05.25 AFV.select :carrierSolution @16:23:05.40 delay 2 seconds @16:23:07.40 AS.reconfigure ASconfig @16:23:07.43 AS.seek 1.000ml @16:24:06.07 delay 1 second @16:24:07.08 AFV.select :holdingCoil @16:24:07.17 delay 1 second @16:24:08.18 AS.reconfigure ASfast @16:24:08.21 AS.seek 0.000ml @16:24:35.94 AFV.select :carrierSolution @16:24:36.04 delay 2 seconds @16:24:38.05 AS.reconfigure ASconfig @16:24:38.08 AS.seek 1.000ml @16:25:36.63 delay 1 second @16:25:37.64 AFV.select :holdingCoil @16:25:37.73 delay 1 second @16:25:38.74 AS.reconfigure ASfast @16:25:38.77 AS.seek 0.000ml @16:26:06.47 ARV.dial between carrier and air1 @16:26:06.96 ATV.dial :air3 @16:26:08.49 AS.reconfigure ASslow1 @16:26:08.53 AS.seek 0.010ml @16:26:10.88 delay 2 seconds @16:26:12.89 ATV.dial :bleach @16:26:13.29 AS.seek 0.810ml @16:28:56.69 delay 3 seconds @16:28:59.70 ATV.dial :air3 @16:29:00.05 delay 3 seconds @16:29:03.11 AS.seek 0.835ml @16:29:08.45 delay 3 seconds @16:29:11.46 ATV.dial :bleach @16:29:11.85 AS.seek 0.820ml @16:29:15.17 delay 3 seconds @16:29:18.18 ATV.dial PCR @16:29:18.51 AS.reconfigure ASconfig @16:29:18.54 AS.seek empty @16:30:06.66 PCR.program :bleachProfile @16:30:06.71 PCR program should complete in < 2:45 @16:30:06.72 PCR.run 10:00 @16:33:07.94 PCR.off @16:33:07.97 AFV.select :carrierSolution @16:33:08.06 delay 2 seconds @16:33:10.07 AS.seek 1.000ml @16:34:08.74 delay 1 second @16:34:09.75 AFV.select :holdingCoil @16:34:09.84 delay 1 second @16:34:10.85 AS.reconfigure ASfast @16:34:10.88 AS.seek 0.000ml @16:34:38.63 delay 3 seconds @16:34:41.64 AFV.select :carrierSolution @16:34:41.73 delay 2 seconds @16:34:43.74 AS.reconfigure ASconfig @16:34:43.77 AS.seek 1.000ml @16:35:42.35 delay 1 second @16:35:43.36 AFV.select :holdingCoil @16:35:43.45 delay 1 second @16:35:44.46 AS.reconfigure ASfast @16:35:44.49 AS.seek 0.000ml @16:36:12.20 delay 3 seconds @16:36:15.21 AFV.select :carrierSolution @16:36:15.30 delay 2 seconds @16:36:17.31 AS.reconfigure ASconfig @16:36:17.34 AS.seek 1.000ml @16:37:15.95 delay 1 second @16:37:16.96 AFV.select :holdingCoil @16:37:17.05 delay 1 second @16:37:18.06 AS.reconfigure ASfast @16:37:18.09 AS.seek 0.000ml @16:37:45.83 delay 3 seconds @16:37:48.84 AFV.select :carrierSolution @16:37:48.93 delay 2 seconds @16:37:50.94 AS.reconfigure ASconfig @16:37:50.97 AS.seek 1.000ml @16:38:49.59 delay 1 second @16:38:50.60 AFV.select :holdingCoil @16:38:50.69 delay 1 second @16:38:51.70 AS.reconfigure ASfast @16:38:51.73 AS.seek 0.000ml @16:39:19.46 delay 3 seconds @16:39:22.47 AFV.select :carrierSolution @16:39:22.56 delay 2 seconds @16:39:24.57 AS.reconfigure ASconfig @16:39:24.60 AS.seek 1.000ml @16:40:23.18 delay 1 second @16:40:24.19 AFV.select :holdingCoil @16:40:24.28 delay 1 second @16:40:25.29 AS.reconfigure ASnorm @16:40:25.32 AS.seek 0.000ml @16:41:16.78 Setting up PCR for IPCap -- writing /var/log/esp/IPCap14feb1716h1ml.pcr @16:41:16.79 Building PCR Slug from ASV with MM1 mix, PP2 primer @16:41:16.80 Priming with carrier ATV.dial ARV @16:41:17.31 ARV.dial :carrier @16:41:17.60 AS.seek 0.500ml @16:41:43.47 AFV.select :carrierSolution @16:41:43.58 delay 2 seconds @16:41:45.59 AS.reconfigure ASconfig @16:41:45.63 AS.seek 1.000ml @16:42:15.06 delay 1 second @16:42:16.07 AFV.select :holdingCoil @16:42:16.17 delay 1 second @16:42:17.18 ATV.dial :waste @16:42:18.45 AS.reconfigure ASnorm @16:42:18.48 AS.seek empty @16:43:09.95 AFV.select :carrierSolution @16:43:10.06 delay 2 seconds @16:43:12.07 AS.reconfigure ASconfig @16:43:12.10 AS.seek 0.500ml @16:43:41.59 delay 1 second @16:43:42.60 AFV.select :holdingCoil @16:43:42.69 Building Slug @16:43:42.70 ATV.dial :ARV @16:43:43.97 delay 2 seconds @16:43:45.98 ARV.dial :air1 @16:43:46.31 delay 2 seconds @16:43:48.37 AS.reconfigure ASslow2 @16:43:48.40 AS.seek 0.506ml @16:43:54.87 delay 2 seconds @16:43:56.88 ARV.dial :MM1 @16:43:57.37 delay 2 seconds @16:43:59.38 Adding MM1 @16:43:59.44 AS.seek 0.524ml @16:44:18.06 delay 25 seconds @16:44:43.07 ARV.dial :PP2 @16:44:43.48 Adding PP2 @16:44:43.50 delay 2 seconds @16:44:45.55 AS.seek 0.530ml @16:44:51.95 delay 10 seconds @16:45:01.96 ARV.dial :air2 @16:45:02.37 adding ARV air @16:45:02.38 delay 2 seconds @16:45:04.44 AS.seek 0.536ml @16:45:10.71 delay 5 seconds @16:45:15.72 ARV.dial :carrier @16:45:16.74 delay 7 seconds @16:45:23.80 AS.seek 0.594ml @16:46:23.34 delay 5 seconds @16:46:28.35 ATV.dial :ASV @16:46:28.68 delay 2 seconds @16:46:30.74 AS.seek 0.600ml @16:46:37.10 delay 4 seconds @16:46:41.11 ATV.dial ARV @16:46:41.44 delay 2 seconds @16:46:44.00 ARV.dial :carrier @16:46:44.01 delay 2 seconds @16:46:46.06 AS.seek 0.650ml @16:47:37.21 delay 4 seconds @16:47:41.22 ATV.dial PCR @16:47:41.72 ASV.dial :waste1 @16:47:41.73 ARV.dial :air1 @16:47:42.08 Positioning Slug by 0.324ml @16:47:42.14 AS.seek 0.326ml @16:53:12.84 delay 5 seconds @16:53:17.85 ATV.dial ARV @16:53:18.37 ASV.dial :PCRlock @16:53:18.85 delay 5 seconds @16:53:23.86 Running IPCap Profile59C thermocycling @16:53:23.87 PCR.program Profile59C @16:53:23.97 Profile59C should complete in < 1:39:08 @16:53:23.98 PCR.run 2:08:55 @18:15:14.66 PCR.off @18:15:15.19 PCR for IPCap completed -- closed /var/log/esp/IPCap14feb1716h1ml.pcr @18:15:15.24 Cleaning PCR @18:15:15.25 AFV.select :carrierSolution @18:15:15.34 delay 2 seconds @18:15:17.35 AS.reconfigure ASconfig @18:15:17.38 AS.seek 0.500ml @18:15:27.81 delay 1 second @18:15:28.82 AFV.select :holdingCoil @18:15:28.91 ASV.dial :waste1 @18:15:29.39 ATV.dial :air3 @18:15:30.33 AS.reconfigure ASslow1 @18:15:30.36 AS.seek 0.510ml @18:15:32.64 delay 2 seconds @18:15:34.65 ATV.dial :bleach @18:15:35.13 AS.seek 0.810ml @18:16:36.55 delay 3 seconds @18:16:39.56 ATV.dial :air3 @18:16:39.89 delay 3 seconds @18:16:42.95 AS.seek 0.835ml @18:16:48.28 delay 3 seconds @18:16:51.29 ATV.dial :bleach @18:16:51.67 AS.seek 0.820ml @18:16:54.98 delay 3 seconds @18:16:57.99 ARV.dial :waste @18:16:59.08 delay 3 seconds @18:17:02.09 ATV.dial :ARV @18:17:02.83 AS.reconfigure ASconfig @18:17:02.86 AS.seek 0.470ml @18:17:23.55 AFV.select :carrierSolution @18:17:23.65 delay 2 seconds @18:17:25.67 AS.seek 1.000ml @18:17:56.90 delay 1 second @18:17:57.91 AFV.select :holdingCoil @18:17:58.00 delay 1 second @18:17:59.01 AS.reconfigure ASfast @18:17:59.04 AS.seek 0.000ml @18:18:26.73 AFV.select :carrierSolution @18:18:26.83 delay 2 seconds @18:18:28.84 AS.reconfigure ASconfig @18:18:28.87 AS.seek 1.000ml @18:19:27.46 delay 1 second @18:19:28.47 AFV.select :holdingCoil @18:19:28.56 delay 1 second @18:19:29.57 AS.reconfigure ASfast @18:19:29.61 AS.seek 0.000ml @18:19:57.32 AFV.select :carrierSolution @18:19:57.43 delay 2 seconds @18:19:59.44 AS.reconfigure ASconfig @18:19:59.47 AS.seek 1.000ml @18:20:58.04 delay 1 second @18:20:59.05 AFV.select :holdingCoil @18:20:59.14 delay 1 second @18:21:00.15 AS.reconfigure ASfast @18:21:00.18 AS.seek 0.000ml @18:21:27.91 ARV.dial between carrier and air1 @18:21:28.32 ATV.dial :air3 @18:21:29.26 AS.reconfigure ASslow1 @18:21:29.29 AS.seek 0.010ml @18:21:31.60 delay 2 seconds @18:21:33.61 ATV.dial :bleach @18:21:34.01 AS.seek 0.810ml @18:24:17.42 delay 3 seconds @18:24:20.43 ATV.dial :air3 @18:24:20.78 delay 3 seconds @18:24:23.84 AS.seek 0.835ml @18:24:29.18 delay 3 seconds @18:24:32.19 ATV.dial :bleach @18:24:32.58 AS.seek 0.820ml @18:24:35.92 delay 3 seconds @18:24:38.93 ATV.dial PCR @18:24:39.25 AS.reconfigure ASconfig @18:24:39.28 AS.seek empty @18:25:27.40 PCR.program :bleachProfile @18:25:27.46 PCR program should complete in < 2:45 @18:25:27.47 PCR.run 10:00 @18:28:28.68 PCR.off @18:28:28.71 AFV.select :carrierSolution @18:28:28.80 delay 2 seconds @18:28:30.81 AS.seek 1.000ml @18:29:29.44 delay 1 second @18:29:30.45 AFV.select :holdingCoil @18:29:30.54 delay 1 second @18:29:31.55 AS.reconfigure ASfast @18:29:31.58 AS.seek 0.000ml @18:29:59.31 delay 3 seconds @18:30:02.32 AFV.select :carrierSolution @18:30:02.41 delay 2 seconds @18:30:04.42 AS.reconfigure ASconfig @18:30:04.45 AS.seek 1.000ml @18:31:03.05 delay 1 second @18:31:04.06 AFV.select :holdingCoil @18:31:04.15 delay 1 second @18:31:05.16 AS.reconfigure ASfast @18:31:05.19 AS.seek 0.000ml @18:31:32.95 delay 3 seconds @18:31:35.96 AFV.select :carrierSolution @18:31:36.05 delay 2 seconds @18:31:38.06 AS.reconfigure ASconfig @18:31:38.09 AS.seek 1.000ml @18:32:36.64 delay 1 second @18:32:37.65 AFV.select :holdingCoil @18:32:37.74 delay 1 second @18:32:38.75 AS.reconfigure ASfast @18:32:38.78 AS.seek 0.000ml @18:33:06.49 delay 3 seconds @18:33:09.50 AFV.select :carrierSolution @18:33:09.59 delay 2 seconds @18:33:11.60 AS.reconfigure ASconfig @18:33:11.63 AS.seek 1.000ml @18:34:10.24 delay 1 second @18:34:11.25 AFV.select :holdingCoil @18:34:11.34 delay 1 second @18:34:12.35 AS.reconfigure ASfast @18:34:12.38 AS.seek 0.000ml @18:34:40.10 delay 3 seconds @18:34:43.11 AFV.select :carrierSolution @18:34:43.20 delay 2 seconds @18:34:45.21 AS.reconfigure ASconfig @18:34:45.24 AS.seek 1.000ml @18:35:43.82 delay 1 second @18:35:44.83 AFV.select :holdingCoil @18:35:44.92 delay 1 second @18:35:45.93 AS.reconfigure ASnorm @18:35:45.96 AS.seek 0.000ml @18:36:37.39 Setting up PCR for IPCem -- writing /var/log/esp/IPCem14feb1718h1ml.pcr @18:36:37.40 Building PCR Slug from ASV with MM3 mix, PP5 primer @18:36:37.41 Priming with carrier @18:36:37.42 ATV.dial ARV @18:36:37.93 ARV.dial :carrier @18:36:38.23 AS.seek 0.500ml @18:37:04.11 AFV.select :carrierSolution @18:37:04.22 delay 2 seconds @18:37:06.23 AS.reconfigure ASconfig @18:37:06.26 AS.seek 1.000ml @18:37:35.71 delay 1 second @18:37:36.72 AFV.select :holdingCoil @18:37:36.82 delay 1 second @18:37:37.83 ATV.dial :waste @18:37:39.11 AS.reconfigure ASnorm @18:37:39.14 AS.seek empty @18:38:30.61 AFV.select :carrierSolution @18:38:30.71 delay 2 seconds @18:38:32.72 AS.reconfigure ASconfig @18:38:32.75 AS.seek 0.500ml @18:39:02.23 delay 1 second @18:39:03.24 AFV.select :holdingCoil @18:39:03.33 Building Slug @18:39:03.34 ATV.dial :ARV @18:39:04.61 delay 2 seconds @18:39:06.62 ARV.dial :air1 @18:39:06.95 delay 2 seconds @18:39:09.01 AS.reconfigure ASslow2 @18:39:09.04 AS.seek 0.506ml @18:39:16.05 delay 2 seconds @18:39:18.06 ARV.dial :MM3 @18:39:18.92 delay 2 seconds @18:39:20.93 Adding MM3 @18:39:20.99 AS.seek 0.524ml @18:39:39.89 delay 25 seconds @18:40:04.91 ARV.dial :PP5 @18:40:05.24 Adding PP5 @18:40:05.26 delay 2 seconds @18:40:07.31 AS.seek 0.530ml @18:40:13.59 delay 10 seconds @18:40:23.60 ARV.dial :air3 @18:40:23.91 adding ARV air @18:40:23.92 delay 2 seconds @18:40:25.98 AS.seek 0.536ml @18:40:32.26 delay 5 seconds @18:40:37.27 ARV.dial :carrier @18:40:38.30 delay 7 seconds @18:40:45.36 AS.seek 0.594ml @18:41:44.75 delay 5 seconds @18:41:49.76 ATV.dial :ASV @18:41:50.08 delay 2 seconds @18:41:52.14 AS.seek 0.600ml @18:41:58.57 delay 4 seconds @18:42:02.58 ATV.dial ARV @18:42:03.02 delay 2 seconds @18:42:05.03 ARV.dial :carrier @18:42:05.04 delay 2 seconds @18:42:07.09 AS.seek 0.650ml @18:42:58.25 delay 4 seconds @18:43:02.26 ATV.dial PCR @18:43:02.77 ASV.dial :waste1 @18:43:02.78 ARV.dial :air1 @18:43:03.12 Positioning Slug by 0.324ml @18:43:03.17 AS.seek 0.326ml @18:48:33.77 delay 5 seconds @18:48:38.78 ATV.dial ARV @18:48:39.29 ASV.dial :PCRlock @18:48:40.05 delay 5 seconds @18:48:45.06 Running IPCem ProfileEM59C thermocycling @18:48:45.07 PCR.program ProfileEM59C @18:48:45.18 ProfileEM59C should complete in < 2:11:38 @18:48:45.19 PCR.run 2:49:32.5 @20:43:11.89 PCR.off @20:43:12.43 PCR for IPCem completed -- closed /var/log/esp/IPCem14feb1718h1ml.pcr @20:43:12.44 Full MFB Decontamination @20:43:12.45 Decon extraction column and decon AIV @20:43:12.46 SPE.hold 2:46:40,55.0C @20:43:12.48 ASV.dial :SPEdelivery @20:43:13.29 delay 20 seconds @20:43:33.30 AFV.select :carrierSolution @20:43:33.40 delay 2 seconds @20:43:35.41 AS.reconfigure ASconfig @20:43:35.44 AS.seek 0.500ml @20:43:45.86 delay 1 second @20:43:46.87 AFV.select :holdingCoil @20:43:46.97 ATV.dial :air1 @20:43:48.49 AS.reconfigure ASslow1 @20:43:48.52 AS.seek 0.520ml @20:43:52.86 ATV.dial :ASV @20:43:54.15 ASV.dial :SPErecovery @20:43:55.15 AS.seek 0.620ml @20:44:15.77 ATV.dial :waste @20:44:17.23 AS.reconfigure ASnorm @20:44:17.26 AS.seek empty @20:44:49.32 AFV.select :holdingCoil @20:44:49.33 ATV.dial :waste @20:44:49.34 AS.reconfigure ASconfig @20:44:49.38 AS.seek 0.000ml @20:44:49.66 AFV.select :carrierSolution @20:44:49.77 delay 2 seconds @20:44:51.78 AS.seek 0.500ml @20:45:21.19 delay 1 second @20:45:22.20 AFV.select :holdingCoil @20:45:22.30 ATV.dial :air3 @20:45:22.88 AS.reconfigure ASslow1 @20:45:22.91 AS.seek 0.520ml @20:45:27.25 ATV.dial :air3 @20:45:27.31 AS.seek 0.530ml @20:45:29.60 delay 2 seconds @20:45:31.61 ATV.dial :bleach @20:45:32.08 AS.seek 0.710ml @20:46:09.06 delay 3 seconds @20:46:12.07 ATV.dial :air3 @20:46:12.39 delay 3 seconds @20:46:15.45 AS.seek 0.735ml @20:46:20.78 delay 3 seconds @20:46:23.79 ATV.dial :bleach @20:46:24.18 AS.seek 0.720ml @20:46:27.47 delay 3 seconds @20:46:30.48 ATV.dial ASV @20:46:31.47 ASV.dial :SPErecovery @20:46:31.53 AS.seek 0.365ml @20:47:44.17 ATV.dial :waste @20:47:45.65 AS.reconfigure ASnorm @20:47:45.68 AS.seek empty @20:48:04.70 ATV.dial :air3 @20:48:05.27 AS.reconfigure ASslow1 @20:48:05.30 AS.seek 0.009ml @20:48:07.65 delay 2 seconds @20:48:09.66 ATV.dial :bleach @20:48:10.03 AS.seek 0.579ml @20:50:06.56 delay 3 seconds @20:50:09.57 ATV.dial :air3 @20:50:09.92 delay 3 seconds @20:50:12.98 AS.seek 0.604ml @20:50:18.34 delay 3 seconds @20:50:21.35 ATV.dial :bleach @20:50:21.75 AS.seek 0.589ml @20:50:25.09 delay 3 seconds @20:50:28.10 ATV.dial :ASV @20:50:29.09 ASV.dial :wasteR @20:50:29.42 AS.seek 0.000ml @20:52:29.85 delay 1:00 @20:53:29.86 ATV.dial ASV @20:53:29.87 AFV.select :carrierSolution @20:53:29.96 delay 2 seconds @20:53:31.97 AS.reconfigure ASconfig @20:53:32.00 AS.seek 1.000ml @20:54:30.58 delay 1 second @20:54:31.59 AFV.select :holdingCoil @20:54:31.69 delay 1 second @20:54:32.70 ASV.dial :wasteR @20:54:32.71 AS.reconfigure ASslow1 @20:54:32.74 AS.seek 0.000ml @20:57:57.10 delay 10 seconds @20:58:07.11 ATV.dial :air3 @20:58:08.23 AS.seek 0.010ml @20:58:10.50 delay 2 seconds @20:58:12.51 ATV.dial :bleach @20:58:12.99 AS.seek 0.580ml @21:00:09.48 delay 3 seconds @21:00:12.49 ATV.dial :air3 @21:00:12.81 delay 3 seconds @21:00:15.87 AS.seek 0.605ml @21:00:21.22 delay 3 seconds @21:00:24.23 ATV.dial :bleach @21:00:24.63 AS.seek 0.590ml @21:00:27.92 delay 3 seconds @21:00:30.93 ATV.dial ASV @21:00:31.80 ASV.dial :SPEdelivery @21:00:32.12 AS.seek 0.000ml @21:02:32.71 delay 1:00 @21:03:32.72 AFV.select :carrierSolution @21:03:32.82 delay 2 seconds @21:03:34.83 AS.reconfigure ASconfig @21:03:34.86 AS.seek 1.000ml @21:04:33.46 delay 1 second @21:04:34.47 AFV.select :holdingCoil @21:04:34.57 delay 1 second @21:04:35.58 ATV.dial ASV @21:04:35.59 ASV.dial :SPEdelivery @21:04:35.60 AS.reconfigure ASslow1 @21:04:35.63 AS.seek 0.000ml @21:07:59.89 delay 10 seconds @21:08:09.90 ATV.dial :air2 @21:08:11.20 AS.reconfigure ASslow0 @21:08:11.23 AS.seek 0.800ml @21:09:33.17 delay 3 seconds @21:09:36.18 ATV.dial :ASV @21:09:36.90 ASV.dial :SPEdelivery @21:09:36.92 AS.reconfigure ASslow1 @21:09:36.95 AS.seek 0.030ml @21:12:14.23 delay 20 seconds @21:12:34.24 ATV.dial :waste,via => air3 @21:12:35.69 AS.reconfigure ASconfig @21:12:35.72 AS.seek empty @21:12:37.81 SPE.coast @21:12:37.89 Removing Bleach from Mixing Coil @21:12:37.90 AFV.select :carrierSolution @21:12:37.99 delay 2 seconds @21:12:40.00 AS.seek 0.500ml @21:13:09.48 delay 1 second @21:13:10.49 AFV.select :holdingCoil @21:13:10.58 ATV.dial :ASV @21:13:12.02 ASV.dial :SPErecovery @21:13:12.73 AS.reconfigure ASslow1 @21:13:12.76 AS.seek 0.730ml @21:13:59.94 ASV.dial :wasteD @21:14:00.45 AS.reconfigure ASnorm @21:14:00.48 AS.seek 0.000ml @21:14:38.08 AFV.select :carrierSolution @21:14:38.18 delay 2 seconds @21:14:40.19 AS.reconfigure ASconfig @21:14:40.22 AS.seek 0.500ml @21:15:09.65 delay 1 second @21:15:10.66 AFV.select :holdingCoil @21:15:10.75 ATV.dial ASV @21:15:10.76 ASV.dial :SPErecovery @21:15:11.75 AS.reconfigure ASslow0 @21:15:11.78 AS.seek 0.300ml @21:15:32.43 ATV.dial :air2 @21:15:33.30 AS.reconfigure ASslow1 @21:15:33.33 AS.seek 0.320ml @21:15:37.68 ATV.dial ASV @21:15:38.44 AS.reconfigure ASslow0 @21:15:38.47 AS.seek 0.570ml @21:16:04.26 ASV.dial :wasteD @21:16:04.77 AS.reconfigure ASnorm @21:16:04.80 AS.seek 0.000ml @21:16:34.32 AFV.select :carrierSolution @21:16:34.42 delay 2 seconds @21:16:36.43 AS.reconfigure ASconfig @21:16:36.46 AS.seek 0.500ml @21:17:05.87 delay 1 second @21:17:06.88 AFV.select :holdingCoil @21:17:06.97 ATV.dial ASV @21:17:06.98 ASV.dial :SPErecovery @21:17:07.69 AS.reconfigure ASslow0 @21:17:07.72 AS.seek 0.300ml @21:17:28.40 ATV.dial :air2 @21:17:29.17 AS.reconfigure ASslow1 @21:17:29.20 AS.seek 0.320ml @21:17:33.54 ATV.dial ASV @21:17:34.31 AS.reconfigure ASslow0 @21:17:34.34 AS.seek 0.570ml @21:18:00.10 ASV.dial :wasteD @21:18:01.03 AS.reconfigure ASnorm @21:18:01.06 AS.seek 0.000ml @21:18:30.54 ATV.dial :air2 @21:18:31.27 AS.reconfigure ASslow0 @21:18:31.30 AS.seek 0.300ml @21:19:02.26 ATV.dial :ASV @21:19:02.98 ASV.dial :wasteD @21:19:03.00 AS.reconfigure ASconfig @21:19:03.03 AS.seek 0.050ml @21:19:17.90 ATV.dial :waste @21:19:19.34 AS.seek 0.000ml @21:19:22.52 ASV.dial :SPEdelivery @21:19:23.65 deconLysateINT @21:19:23.66 ATV.dial :air3 @21:19:24.23 AS.reconfigure ASslow1 @21:19:24.26 AS.seek 0.010ml @21:19:26.63 delay 2 seconds @21:19:28.64 ATV.dial :bleach @21:19:29.02 AS.seek 0.960ml @21:22:43.16 delay 3 seconds @21:22:46.17 ATV.dial :air3 @21:22:46.52 delay 3 seconds @21:22:49.58 AS.seek 0.985ml @21:22:54.94 delay 3 seconds @21:22:57.95 ATV.dial :bleach @21:22:58.35 AS.seek 0.970ml @21:23:01.64 delay 3 seconds @21:23:04.65 ATV.dial :AIV @21:23:05.83 AIV.dial :coilToMFB @21:23:05.84 AWV.select :waste @21:23:05.85 AFV.select :carrierSolution @21:23:05.94 delay 2 seconds @21:23:07.95 AS.reconfigure ASconfig @21:23:07.98 AS.seek 1.000ml @21:23:10.06 delay 1 second @21:23:11.07 AFV.select :holdingCoil @21:23:11.17 delay 1 second @21:23:12.18 AS.reconfigure ASslow0 @21:23:12.21 AS.seek empty @21:24:54.62 AFV.select :carrierSolution @21:24:54.71 delay 2 seconds @21:24:56.72 AS.reconfigure ASconfig @21:24:56.75 AS.seek 1.000ml @21:25:55.42 delay 1 second @21:25:56.43 AFV.select :holdingCoil @21:25:56.53 delay 1 second @21:25:57.54 AS.reconfigure ASslow0 @21:25:57.57 AS.seek empty @21:27:39.97 AFV.select :carrierSolution @21:27:40.06 delay 2 seconds @21:27:42.07 AS.reconfigure ASconfig @21:27:42.10 AS.seek 1.000ml @21:28:40.69 delay 1 second @21:28:41.70 AFV.select :holdingCoil @21:28:41.80 delay 1 second @21:28:42.81 AS.reconfigure ASslow0 @21:28:42.84 AS.seek empty @21:30:25.24 ATV.dial :air2 @21:30:25.83 AS.reconfigure ASslow1 @21:30:25.86 AS.seek 0.009ml @21:30:28.17 AS.reconfigure ASslow0 @21:30:28.21 AS.seek 1.000ml @21:32:10.04 ATV.dial AIV @21:32:10.67 AS.seek 0.020ml @21:33:50.91 ATV.dial :waste @21:33:52.19 AS.seek 0.000ml @21:33:54.52 AWV.select :air @21:33:54.67 Cleaning PCR AFV.select :carrierSolution @21:33:54.76 delay 2 seconds @21:33:56.77 AS.reconfigure ASconfig @21:33:56.80 AS.seek 0.500ml @21:34:26.24 delay 1 second @21:34:27.25 AFV.select :holdingCoil @21:34:27.34 ASV.dial :waste1 @21:34:27.83 ATV.dial :air3 @21:34:28.40 AS.reconfigure ASslow1 @21:34:28.43 AS.seek 0.510ml @21:34:30.72 delay 2 seconds @21:34:32.73 ATV.dial :bleach @21:34:33.10 AS.seek 0.810ml @21:35:34.55 delay 3 seconds @21:35:37.56 ATV.dial :air3 @21:35:37.91 delay 3 seconds @21:35:40.97 AS.seek 0.835ml @21:35:46.34 delay 3 seconds @21:35:49.35 ATV.dial :bleach @21:35:49.74 AS.seek 0.820ml @21:35:53.02 delay 3 seconds @21:35:56.03 ARV.dial :waste @21:35:56.53 delay 3 seconds @21:35:59.54 ATV.dial :ARV @21:36:00.28 AS.reconfigure ASconfig @21:36:00.31 AS.seek 0.470ml @21:36:20.98 AFV.select :carrierSolution @21:36:21.08 delay 2 seconds @21:36:23.09 AS.seek 1.000ml @21:36:54.33 delay 1 second @21:36:55.34 AFV.select :holdingCoil @21:36:55.43 delay 1 second @21:36:56.44 AS.reconfigure ASfast @21:36:56.47 AS.seek 0.000ml @21:37:24.20 AFV.select :carrierSolution @21:37:24.30 delay 2 seconds @21:37:26.31 AS.reconfigure ASconfig @21:37:26.34 AS.seek 1.000ml @21:38:24.95 delay 1 second @21:38:25.96 AFV.select :holdingCoil @21:38:26.05 delay 1 second @21:38:27.06 AS.reconfigure ASfast @21:38:27.09 AS.seek 0.000ml @21:38:54.81 AFV.select :carrierSolution @21:38:54.91 delay 2 seconds @21:38:56.92 AS.reconfigure ASconfig @21:38:56.95 AS.seek 1.000ml @21:39:55.52 delay 1 second @21:39:56.53 AFV.select :holdingCoil @21:39:56.62 delay 1 second @21:39:57.63 AS.reconfigure ASfast @21:39:57.66 AS.seek 0.000ml @21:40:25.40 ARV.dial between carrier and air1 @21:40:25.91 ATV.dial :air3 @21:40:26.87 AS.reconfigure ASslow1 @21:40:26.90 AS.seek 0.010ml @21:40:29.24 delay 2 seconds @21:40:31.25 ATV.dial :bleach @21:40:31.64 AS.seek 0.810ml @21:43:15.05 delay 3 seconds @21:43:18.06 ATV.dial :air3 @21:43:18.41 delay 3 seconds @21:43:21.47 AS.seek 0.835ml @21:43:26.80 delay 3 seconds @21:43:29.81 ATV.dial :bleach @21:43:30.21 AS.seek 0.820ml @21:43:33.52 delay 3 seconds @21:43:36.53 ATV.dial PCR @21:43:36.87 AS.reconfigure ASconfig @21:43:36.90 AS.seek empty @21:44:25.03 PCR.program :bleachProfile @21:44:25.09 PCR program should complete in < 2:45 @21:44:25.10 PCR.run 10:00 @21:47:32.35 PCR.off @21:47:32.38 AFV.select :carrierSolution @21:47:32.47 delay 2 seconds @21:47:34.48 AS.seek 1.000ml @21:48:33.11 delay 1 second @21:48:34.12 AFV.select :holdingCoil @21:48:34.21 delay 1 second @21:48:35.22 AS.reconfigure ASfast @21:48:35.25 AS.seek 0.000ml @21:49:02.97 delay 3 seconds @21:49:05.98 AFV.select :carrierSolution @21:49:06.07 delay 2 seconds @21:49:08.08 AS.reconfigure ASconfig @21:49:08.11 AS.seek 1.000ml @21:50:06.70 delay 1 second @21:50:07.71 AFV.select :holdingCoil @21:50:07.80 delay 1 second @21:50:08.81 AS.reconfigure ASfast @21:50:08.84 AS.seek 0.000ml @21:50:36.58 delay 3 seconds @21:50:39.59 AFV.select :carrierSolution @21:50:39.68 delay 2 seconds @21:50:41.69 AS.reconfigure ASconfig @21:50:41.72 AS.seek 1.000ml @21:51:40.32 delay 1 second @21:51:41.33 AFV.select :holdingCoil @21:51:41.42 delay 1 second @21:51:42.43 AS.reconfigure ASfast @21:51:42.46 AS.seek 0.000ml @21:52:10.20 delay 3 seconds @21:52:13.21 AFV.select :carrierSolution @21:52:13.30 delay 2 seconds @21:52:15.31 AS.reconfigure ASconfig @21:52:15.34 AS.seek 1.000ml @21:53:13.95 delay 1 second @21:53:14.96 AFV.select :holdingCoil @21:53:15.05 delay 1 second @21:53:16.06 AS.reconfigure ASfast @21:53:16.09 AS.seek 0.000ml @21:53:43.81 delay 3 seconds @21:53:46.82 AFV.select :carrierSolution @21:53:46.91 delay 2 seconds @21:53:48.92 AS.reconfigure ASconfig @21:53:48.95 AS.seek 1.000ml @21:54:48.09 delay 1 second @21:54:49.10 AFV.select :holdingCoil @21:54:49.19 delay 1 second @21:54:50.20 AS.reconfigure ASnorm @21:54:50.23 AS.seek 0.000ml @21:55:41.70 AFV.select :carrierSolution @21:55:41.79 delay 2 seconds @21:55:43.80 AS.reconfigure ASconfig @21:55:43.83 AS.seek 1.000ml @21:56:42.42 delay 1 second @21:56:43.43 AFV.select :holdingCoil @21:56:43.52 delay 1 second @21:56:44.53 ATV.dial :waste @21:56:45.45 AS.reconfigure ASnorm @21:56:45.48 AS.seek 0.500ml @21:57:11.42 ATV.dial :waste2 @21:57:12.51 AS.seek 0.000ml @21:57:38.37 AS.stop @21:57:38.39 CC.stop @21:57:38.41 CS.stop @21:57:38.44 PC.stop @21:57:38.47 PS.stop @21:57:38.50 Forearm.stop @21:57:38.53 Elbow.stop @21:57:38.56 SS.stop @21:57:38.63 Sleepy.power :raw,:OFF @21:57:38.64 Sleepy.power :dwarf,:OFF Shutdown @21:57:38.65 GoodNews.email "MFBPCR completed",Subject => "Shutdown" @07:56:52.61PST18-Feb-14 Session Begun @07:57:10.56 -> showlog @08:00:28.39 -> exit @08:00:28.41 Session Ended @08:01:34.57
Powering up Dwarves @08:01:39.76 Sleepy.power :raw,:ON @08:01:40.24 SPE.configure SPEconfig @08:01:40.26 AS.configure ASconfig @08:01:40.30 CC.configure CCconfig @08:01:40.33 CS.configure CSconfig @08:01:40.36 PC.configure PCconfig @08:01:40.39 PS.configure PSconfig @08:01:40.42 Forearm.configure LooseForearm @08:01:40.45 Elbow.configure LooseElbow @08:01:40.48 SC.configure SCloose @08:01:40.51 SE.configure SEconfig @08:01:40.54 SS.configure SSconfig @08:01:40.57 CH.configure CHconfig @08:01:40.59 PH.configure PHconfig @08:01:40.62 Valve.reset @08:01:41.26 PCR.off @08:01:41.29 PCR.pidtemp= 50,0.1,0,300,25,25 @08:01:41.32 PCR.caltemp= 6.6661,0.0344,0.0 @08:01:41.44 Intake.select :close @08:01:41.45 Exhaust.select :close @08:01:41.46 CTV.dial :air @08:01:41.50 PSV.dial :air @08:01:41.53
PC.reconfigure PCconfig @08:01:41.59 PC.home.seek open @08:01:41.61 CSV.dial :waste1 @08:01:42.02 PS.reconfigure PSconfig @08:01:42.05 PS.home.seek 0.000ml @08:01:42.10 SSV.dial :spit,avoiding => exhaust @08:01:42.91 CS.reconfigure CSfast @08:01:42.94 SS.reconfigure SSconfig @08:01:43.04 SS.home.seek 0.00ml @08:01:43.05 CS.home.seek 0.000ml @08:01:43.12 CC.reconfigure CCconfig @08:01:43.16 CC.home.seek open @08:01:52.28 PS.coast @08:01:52.65
CC.seek open @08:01:52.68 PC.seek open @08:01:52.83
Forearm.reconfigure LooseForearm @08:01:52.86 Forearm.home.seek retract @08:01:53.62 Hand.grip @08:01:54.15
Elbow.jog -1500 @08:01:54.66 Elbow.reconfigure LooseElbow @08:01:54.70 Elbow.home.seek clear @08:01:59.49 SE.reconfigure SEconfig @08:01:59.52 SE.home.seek down @08:02:00.08 SC.reconfigure SCsloppy @08:02:00.12 SC.home.seek initial @08:02:02.38 Forearm.seek retract @08:02:02.44 Elbow.seek hammer @08:02:03.63 Forearm.reconfigure TightForearm @08:02:03.68 Forearm.seek 2 @08:02:04.73 Forearm.seek retract @08:02:05.84 Forearm.reconfigure LooseForearm @08:02:06.25PST18-Feb-14 ===> STARTED wqPCR (phase #5) <=== @08:02:06.26 Starting initialPurge @08:02:06.27 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:02:06.32 Hand.release @08:02:07.17 Elbow.reconfigure TightElbow @08:02:07.20 Elbow.seek FlushPuck::Garage @08:02:08.96 Elbow.reconfigure LooseElbow @08:02:09.05 Forearm.seek garage @08:02:10.07 Hand.grip @08:02:10.92 Forearm.seek retract @08:02:11.95 Elbow.reconfigure TightElbow @08:02:11.98 Elbow.seek CC @08:02:14.74 Elbow.reconfigure LooseElbow @08:02:14.82 Forearm.reconfigure TightForearm @08:02:14.85 Forearm.seek CC @08:02:15.87 CC.seek closed @08:02:19.64 Collection Clamp onSpring/offSpring for 4/21 samples, on/off torque 2.13 @08:02:19.69 Hand.release @08:02:20.49 Forearm.reconfigure LooseForearm @08:02:20.52 Forearm.seek retract @08:02:21.51 Flushing the Collection Valve::Manifold @08:02:21.60 CS Pulling 5ml of flush @08:02:21.61 CSV.dial CSR @08:02:22.95 CSR.select :flush @08:02:23.05 CS.reconfigure CSconfig @08:02:23.09 CS.seek 5.000ml @08:02:36.65 delay 5 seconds @08:02:41.66 CSR.select :air @08:02:41.80 CS.reconfigure CSfast @08:02:41.83 CS.seek 7.500ml @08:02:48.84 CSV.dial :waste @08:02:50.18 CS.reconfigure CSconfig @08:02:50.22 CS.seek 0.000ml @08:03:10.21 Purging external Intake @08:03:10.30 CSV.dial :flush @08:03:10.96 CS.reconfigure CSfast @08:03:11.00 CS.seek 10.000ml @08:03:36.55 delay 5 seconds @08:03:41.56 CSV.dial CBV @08:03:41.58
CBV.dial CSV @08:03:42.34 CTV.dial :intake @08:03:43.13 Intake.select :open @08:03:43.22 CS.seek 0.000ml @08:04:09.55 Intake.select :close @08:04:09.64 CTV.dial :air @08:04:10.43 Flushing upper manifold @08:04:10.52 CSV.dial :flush @08:04:11.38 CS.seek 5.000ml @08:04:24.52 delay 5 seconds @08:04:29.53 CSV.dial :air @08:04:30.24 CS.seek 7.000ml @08:04:35.99 CSV.dial CBV @08:04:36.01
CBV.dial CSV @08:04:37.07 CTV.dial :air @08:04:37.09 CS.seek 0.000ml @08:04:55.43 delay 7 seconds @08:05:02.45 CS.seek 3.000ml @08:05:10.68 CSV.dial :waste @08:05:11.22 CS.seek 0.000ml @08:05:12.69 Session Begun @08:05:19.54
CSV.dial :flush @08:05:20.03 CS.seek 3.000ml @08:05:28.30 delay 5 seconds @08:05:33.31 CSV.dial :air @08:05:33.83 CS.seek 5.000ml @08:05:39.64 CSV.dial :air @08:05:39.66 CS.seek 0.000ml @08:05:52.92 delay 7 seconds @08:05:53.82 -> showlog @08:05:59.93
CS.seek 3.000ml @08:06:08.24 CSV.dial :waste @08:06:09.10 CS.seek 0.000ml @08:06:17.30 Garaging FlushPuck in Collection Clamp @08:06:17.38 Elbow.reconfigure TightElbow @08:06:17.42 Elbow.seek CC @08:06:17.65 Elbow.reconfigure LooseElbow @08:06:17.75 Forearm.seek CC @08:06:18.72 CC.seek sealed @08:06:19.67 Hand.grip @08:06:20.44 CC.seek open @08:06:23.73 Forearm.seek retract @08:06:25.27 Elbow.reconfigure TightElbow @08:06:25.31 Elbow.seek garage @08:06:28.12 Elbow.reconfigure LooseElbow @08:06:28.21 Forearm.reconfigure TightForearm @08:06:28.26 Forearm.seek garage @08:06:29.45 Hand.release @08:06:30.23 Forearm.reconfigure LooseForearm @08:06:30.28 Forearm.seek retract @08:06:31.48 Starting mfb @08:06:31.49 pcrBAC sampling at most 1000ml, wcr at most 1000ml @08:06:31.54 Loading sh1 puck from tube 2 into Collection Clamp @08:06:31.60 BACfans.email "Can@08:02:06, 14.6C, 62% humidity, 14.1psia, 12.828V, 0.750A, 0.756A avg, 9.62W\nCTD data unavailable\nISUS data unavailable",Subject => "pcrBAC sampling at most 1000ml, wcr at most 1000ml" @08:06:39.27 Readying the MFB @08:06:39.28 Homing MFB Syringe @08:06:39.29 AFV.select :holdingCoil @08:06:39.34 I2C::Request::Timeout in MAIN -- No Response to I2C::Servo::Status3Request[2B:->27] @08:06:39.35
Retry #1 of 3 @08:06:39.41 I2C::UnexpectedReply in core -- I2C::Servo::Status3Reply[2B:27->20] @08:06:39.55 ATV.dial :air2 @08:06:39.73
SC.reconfigure SCloose @08:06:39.96 Elbow.reconfigure TightElbow @08:06:39.97 AS.reconfigure ASconfig @08:06:40.03
Elbow.seek 2 @08:06:40.06 AS.home.seek home @08:06:41.91
Forearm.reconfigure TightForearm @08:06:41.96 Forearm.seek 2 + 800 counts @08:06:43.12 SE.reconfigure SEpick @08:06:43.19 SE.seek beamBreak @08:06:45.66 SE.configure SEconfig @08:06:45.80 Elbow.seek 2 @08:06:45.95 Forearm.seek 2 + 300 counts @08:06:46.26 Forearm.reconfigure LooseForearm @08:06:46.31 Hand.grip @08:06:47.08 SE.seek down @08:06:47.24
Forearm.seek retract @08:06:47.59 AFV.select :carrierSolution @08:06:47.69 delay 2 seconds @08:06:48.22
Elbow.seek CC @08:06:49.27 SE.coast @08:06:49.76 AS.seek 1.000ml @08:06:49.82
Elbow.reconfigure LooseElbow @08:06:49.92 Forearm.reconfigure TightForearm @08:06:49.97 Forearm.seek CC @08:06:51.17 CC.seek closed @08:06:54.95 Collection Clamp onSpring/offSpring for 4/21 samples, on/off torque 2.28 @08:06:54.96 Hand.release @08:06:55.74 Forearm.reconfigure LooseForearm @08:06:55.78 Forearm.seek retract @08:06:56.73 SC.coast @08:06:56.77 collectSample 1000ml w/bubble point of 55psi @08:06:56.78 Priming 7ml @08:06:56.79 Sleepy.power :sampler,:ON @08:06:56.81 Intake.select :close @08:06:56.82 Exhaust.select :close @08:06:56.84 SS.hold @08:06:57.03 Calibrating pressure and temperature sensors @08:06:57.05 CTV.dial :air @08:06:57.07 SSV.dial :spit,avoiding => exhaust @08:06:57.09 SS.reconfigure SSfast @08:06:57.15 SS.seek 5.00ml @08:07:00.32 SSV.dial CBV @08:07:00.34
CBV.dial SSV @08:07:01.82 delay 3.8 seconds @08:07:05.71 SSV.dial :spit @08:07:07.16 SS.seek 0.00ml @08:07:09.93 SSV.dial CBV @08:07:11.90 CTV.dial :intake @08:07:12.73 Waiting for external sampler to finish priming... @08:07:12.74 delayUntil "08:16:56.79" @08:07:41.53 delay 1 second @08:07:42.54 AFV.select :holdingCoil @08:07:42.67 delay 1 second @08:07:43.68 ATV.dial :waste @08:07:44.63 AS.reconfigure ASnorm @08:07:44.68 AS.home.seek empty @08:08:37.89 ATV.dial :waste @08:08:37.90 ARV.dial between carrier and air1 @08:08:37.98 ASV.dial :waste1 @08:14:55.08 Session Ended @08:16:56.79 SSV.dial CBV @08:16:56.81
CBV.dial SSV @08:16:56.82 Allowing 55s for 7.0ml of remaining 7.0ml Intake.select :open @08:16:58.95 Intake @39.5psia, vacuum from 20.0 to 24.0psi @08:16:58.96 SS.reconfigure forSampling @08:16:59.00 SS.seek 7.00ml @08:17:01.81 Vacuum too high -- waiting up to 40s @08:17:02.09 Intake @32.4psia, vacuum from 20.0 to 24.0psi @08:17:02.10 SS.reconfigure forSampling @08:17:02.13 SS.seek 7.00ml @08:17:05.29 Stroke completed -- waiting for pressure to equalize ... @08:17:05.93 Intake.select :close @08:17:06.06 SSV.dial :exhaust @08:17:06.86 Exhaust.select :open @08:17:06.97 SS.reconfigure SSexhale @08:17:07.01 SS.seek 0.00ml @08:17:11.09 Exhaust.select :close @08:17:11.19 Sampling 1000.0ml @08:17:11.20 Intake.select :close @08:17:11.21 Exhaust.select :close @08:17:11.22 SS.reconfigure SSconfig @08:17:11.25 SS.hold @08:17:11.40 CTV.dial :intake @08:17:11.41 SSV.dial CBV @08:17:11.43
CBV.dial SSV @08:17:12.26 Allowing 185s for 25.0ml of remaining 1000.0ml Intake.select :open @08:17:14.40 Intake @39.7psia, vacuum from 20.0 to 24.0psi @08:17:14.41 SS.reconfigure forSampling @08:17:14.44 SS.seek 25.00ml @08:17:22.59 Vacuum too high -- waiting up to 40s @08:17:22.85 Intake @32.5psia, vacuum from 20.0 to 24.0psi @08:17:22.86 SS.reconfigure forSampling @08:17:22.89 SS.seek 25.00ml @08:17:31.12 Stroke completed -- waiting for pressure to equalize ... @08:17:31.76 Intake.select :close @08:17:31.89 SSV.dial :exhaust @08:17:32.68 Exhaust.select :open @08:17:32.77 SS.reconfigure SSexhale @08:17:32.81 SS.seek 0.00ml @08:17:41.78 Exhaust.select :close @08:17:41.87 SSV.dial CBV @08:17:41.89
CBV.dial SSV @08:17:42.70 Allowing 185s for 25.0ml of remaining 975.0ml @08:17:42.71 Intake.select :open @08:17:44.84 Intake @40.7psia, vacuum from 20.0 to 24.0psi @08:17:44.85 SS.reconfigure forSampling @08:17:44.89 SS.seek 25.00ml @08:17:50.90 Vacuum too high -- waiting up to 40s @08:17:51.18 Intake @33.3psia, vacuum from 20.0 to 24.0psi @08:17:51.19 SS.reconfigure forSampling @08:17:51.22 SS.seek 25.00ml @08:18:03.71 Stroke completed -- waiting for pressure to equalize ... @08:18:04.35 Intake.select :close @08:18:04.48 SSV.dial :exhaust @08:18:05.28 Exhaust.select :open @08:18:05.37 SS.reconfigure SSexhale @08:18:05.41 SS.seek 0.00ml @08:18:14.38 Exhaust.select :close @08:18:14.47 SSV.dial CBV @08:18:14.49
CBV.dial SSV @08:18:15.31 Allowing 185s for 25.0ml of remaining 950.0ml @08:18:15.32 Intake.select :open @08:18:17.45 Intake @39.3psia, vacuum from 20.0 to 24.0psi @08:18:17.46 SS.reconfigure forSampling @08:18:17.50 SS.seek 25.00ml @08:18:31.53 Vacuum too high -- waiting up to 40s @08:18:31.79 Intake @34.5psia, vacuum from 20.0 to 24.0psi @08:18:31.81 SS.reconfigure forSampling @08:18:31.84 SS.seek 25.00ml @08:18:37.83 Stroke completed -- waiting for pressure to equalize ... @08:18:38.47 Intake.select :close @08:18:38.60 SSV.dial :exhaust @08:18:39.39 Exhaust.select :open @08:18:39.48 SS.reconfigure SSexhale @08:18:39.52 SS.seek 0.00ml @08:18:48.30 Exhaust.select :close @08:18:48.39 SSV.dial CBV @08:18:48.41
CBV.dial SSV @08:18:49.22 Allowing 185s for 25.0ml of remaining 925.0ml @08:18:49.23 Intake.select :open @08:18:51.36 Intake @39.8psia, vacuum from 20.0 to 24.0psi @08:18:51.37 SS.reconfigure forSampling @08:18:51.41 SS.seek 25.00ml @08:19:05.90 Vacuum too high -- waiting up to 40s @08:19:06.17 Intake @35.9psia, vacuum from 20.0 to 24.0psi @08:19:06.18 SS.reconfigure forSampling @08:19:06.21 SS.seek 25.00ml @08:19:14.81 Stroke completed -- waiting for pressure to equalize ... @08:19:15.45 Intake.select :close @08:19:15.58 SSV.dial :exhaust @08:19:16.38 Exhaust.select :open @08:19:16.47 SS.reconfigure SSexhale @08:19:16.51 SS.seek 0.00ml @08:19:25.50 Exhaust.select :close @08:19:25.59 SSV.dial CBV @08:19:25.61
CBV.dial SSV @08:19:26.42 Allowing 185s for 25.0ml of remaining 900.0ml @08:19:26.43 Intake.select :open @08:19:28.56 Intake @41.1psia, vacuum from 20.0 to 24.0psi @08:19:28.57 SS.reconfigure forSampling @08:19:28.61 SS.seek 25.00ml @08:19:33.43 Vacuum too high -- waiting up to 40s @08:19:33.70 Intake @36.4psia, vacuum from 20.0 to 24.0psi @08:19:33.71 SS.reconfigure forSampling @08:19:33.75 SS.seek 25.00ml @08:19:59.37 Stroke completed -- waiting for pressure to equalize ... @08:20:00.01 Intake.select :close @08:20:00.14 SSV.dial :exhaust @08:20:00.94 Exhaust.select :open @08:20:01.04 SS.reconfigure SSexhale @08:20:01.08 SS.seek 0.00ml @08:20:10.17 Exhaust.select :close @08:20:10.26 SSV.dial CBV @08:20:10.28
CBV.dial SSV @08:20:11.09 Allowing 185s for 25.0ml of remaining 875.0ml @08:20:11.10 Intake.select :open @08:20:13.23 Intake @40.3psia, vacuum from 20.0 to 24.0psi @08:20:13.24 SS.reconfigure forSampling @08:20:13.28 SS.seek 25.00ml @08:20:18.27 Vacuum too high -- waiting up to 40s @08:20:18.54 Intake @37.9psia, vacuum from 20.0 to 24.0psi @08:20:18.55 SS.reconfigure forSampling @08:20:18.58 SS.seek 25.00ml @08:20:53.01 Stroke completed -- waiting for pressure to equalize ... @08:20:53.65 Intake.select :close @08:20:53.78 SSV.dial :exhaust @08:20:54.57 Exhaust.select :open @08:20:54.68 SS.reconfigure SSexhale @08:20:54.72 SS.seek 0.00ml @08:21:03.56 Exhaust.select :close @08:21:03.65 SSV.dial CBV @08:21:03.67
CBV.dial SSV @08:21:04.48 Allowing 185s for 25.0ml of remaining 850.0ml @08:21:04.49 Intake.select :open @08:21:06.62 Intake @39.8psia, vacuum from 20.0 to 24.0psi @08:21:06.63 SS.reconfigure forSampling @08:21:06.67 SS.seek 25.00ml @08:21:17.52 Vacuum too high -- waiting up to 40s @08:21:17.79 Intake @38.7psia, vacuum from 20.0 to 24.0psi @08:21:17.80 SS.reconfigure forSampling @08:21:17.83 SS.seek 25.00ml @08:22:01.27 Stroke completed -- waiting for pressure to equalize ... @08:22:01.91 Intake.select :close @08:22:02.04 SSV.dial :exhaust @08:22:02.84 Exhaust.select :open @08:22:02.93 SS.reconfigure SSexhale @08:22:02.97 SS.seek 0.00ml @08:22:11.68 Exhaust.select :close @08:22:11.77 SSV.dial CBV @08:22:11.79
CBV.dial SSV @08:22:12.60 Allowing 185s for 25.0ml of remaining 825.0ml @08:22:12.61 Intake.select :open @08:22:14.74 Intake @40.4psia, vacuum from 20.0 to 24.0psi @08:22:14.75 SS.reconfigure forSampling @08:22:14.79 SS.seek 25.00ml @08:22:37.39 Vacuum too high -- waiting up to 40s @08:22:37.66 Intake @39.1psia, vacuum from 20.0 to 24.0psi @08:22:37.67 SS.reconfigure forSampling @08:22:37.70 SS.seek 25.00ml @08:23:46.19 Stroke completed -- waiting for pressure to equalize ... @08:23:47.41 Intake.select :close @08:23:47.54 SSV.dial :exhaust @08:23:48.36 Exhaust.select :open @08:23:48.46 SS.reconfigure SSexhale @08:23:48.50 SS.seek 0.00ml @08:23:57.39 Exhaust.select :close @08:23:57.48 SSV.dial CBV @08:23:57.50
CBV.dial SSV @08:23:58.33 Allowing 185s for 25.0ml of remaining 800.0ml @08:23:58.34 Intake.select :open @08:24:00.47 Intake @40.8psia, vacuum from 20.0 to 24.0psi @08:24:00.48 SS.reconfigure forSampling @08:24:00.52 SS.seek 25.00ml @08:25:51.73 Stroke completed -- waiting for pressure to equalize ... @08:25:52.37 Intake.select :close @08:25:52.50 SSV.dial :exhaust @08:25:53.31 Exhaust.select :open @08:25:53.41 SS.reconfigure SSexhale @08:25:53.45 SS.seek 0.00ml @08:26:02.54 Exhaust.select :close @08:26:02.63 SSV.dial CBV @08:26:02.65
CBV.dial SSV @08:26:03.48 Allowing 185s for 25.0ml of remaining 775.0ml @08:26:03.49 Intake.select :open @08:26:05.62 Intake @41.0psia, vacuum from 20.0 to 24.0psi @08:26:05.63 SS.reconfigure forSampling @08:26:05.67 SS.seek 25.00ml @08:27:26.53 Vacuum too high -- waiting up to 40s @08:27:27.02 Intake @39.2psia, vacuum from 20.0 to 24.0psi @08:27:27.03 SS.reconfigure forSampling @08:27:27.06 SS.seek 25.00ml @08:27:56.80 Stroke completed -- waiting for pressure to equalize ... @08:27:58.61 Intake.select :close @08:27:58.74 SSV.dial :exhaust @08:27:59.55 Exhaust.select :open @08:27:59.65 SS.reconfigure SSexhale @08:27:59.69 SS.seek 0.00ml @08:28:08.52 Exhaust.select :close @08:28:08.61 SSV.dial CBV @08:28:08.63
CBV.dial SSV @08:28:09.46 Allowing 185s for 25.0ml of remaining 750.0ml @08:28:09.47 Intake.select :open @08:28:11.60 Intake @39.7psia, vacuum from 20.0 to 24.0psi @08:28:11.61 SS.reconfigure forSampling @08:28:11.65 SS.seek 25.00ml @08:28:51.00 Vacuum too high -- waiting up to 40s @08:28:52.02 Intake @39.5psia, vacuum from 20.0 to 24.0psi @08:28:52.04 SS.reconfigure forSampling @08:28:52.07 SS.seek 25.00ml @08:29:06.25 Vacuum too high -- waiting up to 40s @08:29:06.73 Intake @38.7psia, vacuum from 20.0 to 24.0psi @08:29:06.74 SS.reconfigure forSampling @08:29:06.77 SS.seek 25.00ml @08:29:29.40 Vacuum too high -- waiting up to 40s @08:29:29.67 Intake @38.3psia, vacuum from 20.0 to 24.0psi @08:29:29.68 SS.reconfigure forSampling @08:29:29.71 SS.seek 25.00ml @08:29:37.84 Vacuum too high -- waiting up to 40s @08:29:38.32 Intake @40.3psia, vacuum from 20.0 to 24.0psi @08:29:38.33 SS.reconfigure forSampling @08:29:38.37 SS.seek 25.00ml @08:29:40.72 Vacuum too high -- waiting up to 40s @08:29:41.20 Intake @40.2psia, vacuum from 20.0 to 24.0psi @08:29:41.21 SS.reconfigure forSampling @08:29:41.25 SS.seek 25.00ml @08:29:43.54 Vacuum too high -- waiting up to 40s @08:29:44.03 Intake @39.5psia, vacuum from 20.0 to 24.0psi @08:29:44.04 SS.reconfigure forSampling @08:29:44.07 SS.seek 25.00ml @08:29:46.44 Vacuum too high -- waiting up to 40s @08:29:46.92 SS.seek 25.00ml @08:29:48.97 Vacuum too high -- waiting up to 40s @08:29:49.45 Intake @39.6psia, vacuum from 20.0 to 24.0psi @08:29:49.46 SS.reconfigure forSampling @08:29:49.50 SS.seek 25.00ml @08:29:52.13 Vacuum too high -- waiting up to 40s @08:29:52.60 Intake @40.7psia, vacuum from 20.0 to 24.0psi @08:29:52.61 SS.reconfigure forSampling @08:29:52.65 SS.seek 25.00ml @08:29:54.50 Vacuum too high -- waiting up to 40s @08:29:54.97 Intake @39.6psia, vacuum from 20.0 to 24.0psi @08:29:54.98 SS.reconfigure forSampling @08:29:55.01 SS.seek 25.00ml @08:29:57.10 Vacuum too high -- waiting up to 40s @08:29:57.58 Intake @39.5psia, vacuum from 20.0 to 24.0psi @08:29:57.59 SS.reconfigure forSampling @08:29:57.63 SS.seek 25.00ml @08:29:59.57 Vacuum too high -- waiting up to 40s @08:30:00.05 Intake @38.5psia, vacuum from 20.0 to 24.0psi @08:30:00.06 SS.reconfigure forSampling @08:30:00.09 SS.seek 25.00ml @08:30:04.90 Vacuum too high -- waiting up to 40s @08:30:05.39 Intake @39.5psia, vacuum from 20.0 to 24.0psi @08:30:05.40 SS.reconfigure forSampling @08:30:05.43 SS.seek 25.00ml @08:30:07.38 Vacuum too high -- waiting up to 40s @08:30:07.85 Intake @39.3psia, vacuum from 20.0 to 24.0psi @08:30:07.86 SS.reconfigure forSampling @08:30:07.89 SS.seek 25.00ml @08:30:09.91 Vacuum too high -- waiting up to 40s @08:30:10.17 Intake @38.8psia, vacuum from 20.0 to 24.0psi @08:30:10.18 SS.reconfigure forSampling @08:30:10.21 SS.seek 25.00ml @08:30:12.03 Vacuum too high -- waiting up to 40s @08:30:12.51 Intake @39.3psia, vacuum from 20.0 to 24.0psi @08:30:12.52 SS.reconfigure forSampling @08:30:12.56 SS.seek 25.00ml @08:30:14.97 Stroke completed -- waiting for pressure to equalize ... @08:30:16.20 Intake.select :close @08:30:16.33 SSV.dial :exhaust @08:30:17.14 Exhaust.select :open @08:30:17.24 SS.reconfigure SSexhale @08:30:17.28 SS.seek 0.00ml @08:30:25.86 Exhaust.select :close @08:30:25.95 SSV.dial CBV @08:30:25.97
CBV.dial SSV @08:30:26.79 Allowing 185s for 25.0ml of remaining 725.0ml @08:30:26.80 Intake.select :open @08:30:28.93 Intake @40.3psia, vacuum from 20.0 to 24.0psi @08:30:28.94 SS.reconfigure forSampling @08:30:28.98 SS.seek 25.00ml @08:30:31.47 Vacuum too high -- waiting up to 40s @08:30:31.95 Intake @38.8psia, vacuum from 20.0 to 24.0psi @08:30:31.96 SS.reconfigure forSampling @08:30:31.99 SS.seek 25.00ml @08:30:34.44 Vacuum too high -- waiting up to 40s @08:30:34.92 Intake @38.6psia, vacuum from 20.0 to 24.0psi @08:30:34.93 SS.reconfigure forSampling @08:30:34.96 SS.seek 25.00ml @08:30:37.25 Vacuum too high -- waiting up to 40s @08:30:37.72 Intake @39.1psia, vacuum from 20.0 to 24.0psi @08:30:37.73 SS.reconfigure forSampling @08:30:37.77 SS.seek 25.00ml @08:30:40.53 Vacuum too high -- waiting up to 40s @08:30:41.01 Intake @37.2psia, vacuum from 20.0 to 24.0psi @08:30:41.02 SS.reconfigure forSampling @08:30:41.05 SS.seek 25.00ml @08:30:58.59 Vacuum too high -- waiting up to 40s @08:30:59.28 Intake @39.2psia, vacuum from 20.0 to 24.0psi @08:30:59.29 SS.reconfigure forSampling @08:30:59.32 SS.seek 25.00ml @08:31:01.63 Vacuum too high -- waiting up to 40s @08:31:02.10 Intake @39.5psia, vacuum from 20.0 to 24.0psi @08:31:02.11 SS.reconfigure forSampling @08:31:02.15 SS.seek 25.00ml @08:31:04.13 Vacuum too high -- waiting up to 40s @08:31:04.60 Intake @39.6psia, vacuum from 20.0 to 24.0psi @08:31:04.61 SS.reconfigure forSampling @08:31:04.64 SS.seek 25.00ml @08:31:06.66 Vacuum too high -- waiting up to 40s @08:31:07.13 Intake @39.4psia, vacuum from 20.0 to 24.0psi @08:31:07.14 SS.reconfigure forSampling @08:31:07.18 SS.seek 25.00ml @08:31:09.81 Vacuum too high -- waiting up to 40s @08:31:10.50 Intake @41.1psia, vacuum from 20.0 to 24.0psi @08:31:10.51 SS.reconfigure forSampling @08:31:10.54 SS.seek 25.00ml @08:31:12.38 Vacuum too high -- waiting up to 40s @08:31:12.86 Intake @40.4psia, vacuum from 20.0 to 24.0psi @08:31:12.87 SS.reconfigure forSampling @08:31:12.91 SS.seek 25.00ml @08:31:14.81 Vacuum too high -- waiting up to 40s @08:31:15.29 Intake @39.8psia, vacuum from 20.0 to 24.0psi SS.reconfigure forSampling @08:31:15.33 SS.seek 25.00ml @08:31:17.25 Vacuum too high -- waiting up to 40s @08:31:17.73 Intake @39.9psia, vacuum from 20.0 to 24.0psi @08:31:17.74 SS.reconfigure forSampling @08:31:17.77 SS.seek 25.00ml @08:31:19.53 Vacuum too high -- waiting up to 40s @08:31:20.00 Intake @39.5psia, vacuum from 20.0 to 24.0psi @08:31:20.01 SS.reconfigure forSampling @08:31:20.04 SS.seek 25.00ml @08:31:21.78 Vacuum too high -- waiting up to 40s @08:31:22.48 Intake @40.2psia, vacuum from 20.0 to 24.0psi @08:31:22.49 SS.reconfigure forSampling @08:31:22.53 SS.seek 25.00ml @08:31:24.72 Vacuum too high -- waiting up to 40s @08:31:25.20 Intake @38.7psia, vacuum from 20.0 to 24.0psi @08:31:25.21 SS.reconfigure forSampling @08:31:25.24 SS.seek 25.00ml @08:31:27.34 Vacuum too high -- waiting up to 40s @08:31:28.04 Intake @40.0psia, vacuum from 20.0 to 24.0psi @08:31:28.05 SS.reconfigure forSampling @08:31:28.08 SS.seek 25.00ml @08:31:29.91 Vacuum too high -- waiting up to 40s @08:31:30.39 Intake @39.1psia, vacuum from 20.0 to 24.0psi @08:31:30.40 SS.reconfigure forSampling @08:31:30.43 SS.seek 25.00ml @08:31:32.31 Vacuum too high -- waiting up to 40s @08:31:32.79 Intake @39.2psia, vacuum from 20.0 to 24.0psi @08:31:32.80 SS.reconfigure forSampling @08:31:32.83 SS.seek 25.00ml @08:31:34.50 Vacuum too high -- waiting up to 40s @08:31:34.98 Intake @39.1psia, vacuum from 20.0 to 24.0psi @08:31:34.99 SS.reconfigure forSampling @08:31:35.02 SS.seek 25.00ml @08:31:36.78 Vacuum too high -- waiting up to 40s @08:31:37.47 Intake @40.6psia, vacuum from 20.0 to 24.0psi @08:31:37.48 SS.reconfigure forSampling @08:31:37.51 SS.seek 25.00ml @08:31:39.31 Vacuum too high -- waiting up to 40s @08:31:40.40 Intake @39.5psia, vacuum from 20.0 to 24.0psi @08:31:40.41 SS.reconfigure forSampling @08:31:40.45 SS.seek 25.00ml @08:31:42.59 Vacuum too high -- waiting up to 40s @08:31:43.07 Intake @39.3psia, vacuum from 20.0 to 24.0psi @08:31:43.08 SS.reconfigure forSampling @08:31:43.12 SS.seek 25.00ml @08:31:44.84 Vacuum too high -- waiting up to 40s @08:31:45.33 Intake @39.5psia, vacuum from 20.0 to 24.0psi @08:31:45.34 SS.reconfigure forSampling @08:31:45.37 SS.seek 25.00ml @08:31:47.00 Vacuum too high -- waiting up to 40s @08:31:47.47 Intake @40.1psia, vacuum from 20.0 to 24.0psi @08:31:47.48 SS.reconfigure forSampling @08:31:47.51 SS.seek 25.00ml @08:31:49.22 Vacuum too high -- waiting up to 40s @08:31:49.69 Intake @39.8psia, vacuum from 20.0 to 24.0psi @08:31:49.70 SS.reconfigure forSampling @08:31:49.73 SS.seek 25.00ml @08:31:51.50 Vacuum too high -- waiting up to 40s @08:31:51.99 Intake @39.5psia, vacuum from 20.0 to 24.0psi @08:31:52.00 SS.reconfigure forSampling @08:31:52.04 SS.seek 25.00ml @08:31:53.53 Vacuum too high -- waiting up to 40s @08:31:54.00 Intake @38.7psia, vacuum from 20.0 to 24.0psi @08:31:54.01 SS.reconfigure forSampling @08:31:54.05 SS.seek 25.00ml @08:31:55.75 Vacuum too high -- waiting up to 40s @08:31:56.22 Intake @39.3psia, vacuum from 20.0 to 24.0psi @08:31:56.23 SS.reconfigure forSampling @08:31:56.27 SS.seek 25.00ml @08:31:57.94 Vacuum too high -- waiting up to 40s @08:31:58.62 Intake @40.3psia, vacuum from 20.0 to 24.0psi @08:31:58.63 SS.reconfigure forSampling @08:31:58.66 SS.seek 25.00ml @08:32:00.59 Vacuum too high -- waiting up to 40s @08:32:01.28 Intake @33.4psia, vacuum from 20.0 to 24.0psi @08:32:01.29 SS.reconfigure forSampling @08:32:01.33 SS.seek 25.00ml @08:32:45.04 Stroke completed -- waiting for pressure to equalize ... @08:32:47.44 Intake.select :close @08:32:47.57 SSV.dial :exhaust @08:32:48.38 Exhaust.select :open @08:32:48.48 SS.reconfigure SSexhale @08:32:48.52 SS.seek 0.00ml @08:32:57.24 Exhaust.select :close @08:32:57.33 SSV.dial CBV @08:32:57.35
CBV.dial SSV @08:32:58.18 Allowing 185s for 25.0ml of remaining 700.0ml @08:32:58.19 Intake.select :open @08:33:00.32 Intake @40.8psia, vacuum from 20.0 to 24.0psi @08:33:00.33 SS.reconfigure forSampling @08:33:00.37 SS.seek 25.00ml @08:33:02.34 Vacuum too high -- waiting up to 40s @08:33:02.60 Intake @38.2psia, vacuum from 20.0 to 24.0psi @08:33:02.61 SS.reconfigure forSampling @08:33:02.64 SS.seek 25.00ml @08:33:03.74 Vacuum too high -- waiting up to 40s @08:33:04.22 Intake @37.9psia, vacuum from 20.0 to 24.0psi @08:33:04.23 SS.reconfigure forSampling @08:33:04.27 SS.seek 25.00ml @08:33:05.62 Vacuum too high -- waiting up to 40s @08:33:06.10 Intake @37.7psia, vacuum from 20.0 to 24.0psi @08:33:06.11 SS.reconfigure forSampling @08:33:06.14 SS.seek 25.00ml @08:33:07.65 Vacuum too high -- waiting up to 40s @08:33:08.13 Intake @38.0psia, vacuum from 20.0 to 24.0psi @08:33:08.14 SS.reconfigure forSampling @08:33:08.17 SS.seek 25.00ml @08:33:09.62 Vacuum too high -- waiting up to 40s @08:33:10.31 Intake @38.5psia, vacuum from 20.0 to 24.0psi @08:33:10.32 SS.reconfigure forSampling @08:33:10.36 SS.seek 25.00ml @08:33:12.09 Vacuum too high -- waiting up to 40s @08:33:12.56 Intake @38.4psia, vacuum from 20.0 to 24.0psi @08:33:12.57 SS.reconfigure forSampling @08:33:12.60 SS.seek 25.00ml @08:33:14.06 Vacuum too high -- waiting up to 40s @08:33:15.34 Intake @38.6psia, vacuum from 20.0 to 24.0psi @08:33:15.35 SS.reconfigure forSampling @08:33:15.38 SS.seek 25.00ml @08:33:17.62 Vacuum too high -- waiting up to 40s @08:33:18.31 Intake @39.0psia, vacuum from 20.0 to 24.0psi @08:33:18.32 SS.reconfigure forSampling @08:33:18.35 SS.seek 25.00ml @08:33:20.03 Vacuum too high -- waiting up to 40s @08:33:20.73 Intake @39.6psia, vacuum from 20.0 to 24.0psi @08:33:20.74 SS.reconfigure forSampling @08:33:20.77 SS.seek 25.00ml @08:33:22.49 Vacuum too high -- waiting up to 40s @08:33:23.19 Intake @38.0psia, vacuum from 20.0 to 24.0psi @08:33:23.20 SS.reconfigure forSampling @08:33:23.23 SS.seek 25.00ml @08:33:24.96 Vacuum too high -- waiting up to 40s @08:33:25.64 Intake @38.0psia, vacuum from 20.0 to 24.0psi @08:33:25.65 SS.reconfigure forSampling @08:33:25.68 SS.seek 25.00ml @08:33:27.27 Vacuum too high -- waiting up to 40s @08:33:27.95 Intake @38.2psia, vacuum from 20.0 to 24.0psi @08:33:27.96 SS.reconfigure forSampling @08:33:27.99 SS.seek 25.00ml @08:33:29.52 Vacuum too high -- waiting up to 40s @08:33:30.20 Intake @39.0psia, vacuum from 20.0 to 24.0psi @08:33:30.21 SS.reconfigure forSampling @08:33:30.24 SS.seek 25.00ml @08:33:31.81 Vacuum too high -- waiting up to 40s @08:33:32.30 Intake @38.5psia, vacuum from 20.0 to 24.0psi @08:33:32.31 SS.reconfigure forSampling @08:33:32.34 SS.seek 25.00ml @08:33:33.74 Vacuum too high -- waiting up to 40s @08:33:34.42 Intake @38.4psia, vacuum from 20.0 to 24.0psi @08:33:34.43 SS.reconfigure forSampling @08:33:34.47 SS.seek 25.00ml @08:33:36.19 Vacuum too high -- waiting up to 40s @08:33:36.67 Intake @38.1psia, vacuum from 20.0 to 24.0psi @08:33:36.68 SS.reconfigure forSampling @08:33:36.71 SS.seek 25.00ml @08:33:38.18 Vacuum too high -- waiting up to 40s @08:33:38.87 Intake @38.2psia, vacuum from 20.0 to 24.0psi @08:33:38.88 SS.reconfigure forSampling @08:33:38.91 SS.seek 25.00ml @08:33:40.40 Vacuum too high -- waiting up to 40s @08:33:40.87 Intake @37.7psia, vacuum from 20.0 to 24.0psi @08:33:40.88 SS.reconfigure forSampling @08:33:40.91 SS.seek 25.00ml @08:33:42.31 Vacuum too high -- waiting up to 40s @08:33:43.00 Intake @38.5psia, vacuum from 20.0 to 24.0psi @08:33:43.01 SS.reconfigure forSampling @08:33:43.04 SS.seek 25.00ml @08:33:44.56 Vacuum too high -- waiting up to 40s @08:33:45.25 Intake @38.9psia, vacuum from 20.0 to 24.0psi @08:33:45.26 SS.reconfigure forSampling @08:33:45.29 SS.seek 25.00ml @08:33:46.81 Vacuum too high -- waiting up to 40s @08:33:47.28 Intake @37.5psia, vacuum from 20.0 to 24.0psi @08:33:47.29 SS.reconfigure forSampling @08:33:47.32 SS.seek 25.00ml @08:33:48.84 Vacuum too high -- waiting up to 40s @08:33:49.32 Intake @38.1psia, vacuum from 20.0 to 24.0psi @08:33:49.33 SS.reconfigure forSampling @08:33:49.36 SS.seek 25.00ml @08:33:50.52 Vacuum too high -- waiting up to 40s @08:33:51.00 Intake @38.0psia, vacuum from 20.0 to 24.0psi @08:33:51.01 SS.reconfigure forSampling @08:33:51.04 SS.seek 25.00ml @08:33:52.43 Vacuum too high -- waiting up to 40s @08:33:53.11 Intake @39.6psia, vacuum from 20.0 to 24.0psi @08:33:53.12 SS.reconfigure forSampling @08:33:53.15 SS.seek 25.00ml @08:33:55.34 Vacuum too high -- waiting up to 40s @08:33:55.60 Intake @37.2psia, vacuum from 20.0 to 24.0psi @08:33:55.61 SS.reconfigure forSampling @08:33:55.64 SS.seek 25.00ml @08:33:56.77 Vacuum too high -- waiting up to 40s @08:33:57.25 Intake @38.0psia, vacuum from 20.0 to 24.0psi @08:33:57.27 SS.reconfigure forSampling @08:33:57.30 SS.seek 25.00ml @08:33:58.43 Vacuum too high -- waiting up to 40s @08:33:58.91 Intake @37.4psia, vacuum from 20.0 to 24.0psi @08:33:58.92 SS.reconfigure forSampling @08:33:58.96 SS.seek 25.00ml @08:34:00.37 Vacuum too high -- waiting up to 40s @08:34:00.85 Intake @38.1psia, vacuum from 20.0 to 24.0psi @08:34:00.86 SS.reconfigure forSampling @08:34:00.89 SS.seek 25.00ml @08:34:02.12 Vacuum too high -- waiting up to 40s @08:34:02.59 Intake @37.7psia, vacuum from 20.0 to 24.0psi @08:34:02.60 SS.reconfigure forSampling @08:34:02.64 SS.seek 25.00ml @08:34:03.90 Vacuum too high -- waiting up to 40s @08:34:04.59 Intake @39.2psia, vacuum from 20.0 to 24.0psi @08:34:04.60 SS.reconfigure forSampling @08:34:04.64 SS.seek 25.00ml @08:34:06.18 Vacuum too high -- waiting up to 40s @08:34:06.66 Intake @38.5psia, vacuum from 20.0 to 24.0psi @08:34:06.67 SS.reconfigure forSampling @08:34:06.70 SS.seek 25.00ml @08:34:07.80 Vacuum too high -- waiting up to 40s @08:34:08.49 Intake @38.8psia, vacuum from 20.0 to 24.0psi @08:34:08.50 SS.reconfigure forSampling @08:34:08.53 SS.seek 25.00ml @08:34:09.96 Vacuum too high -- waiting up to 40s @08:34:10.66 Intake @39.3psia, vacuum from 20.0 to 24.0psi @08:34:10.67 SS.reconfigure forSampling @08:34:10.70 SS.seek 25.00ml @08:34:12.18 Vacuum too high -- waiting up to 40s @08:34:12.86 Intake @39.6psia, vacuum from 20.0 to 24.0psi @08:34:12.87 SS.reconfigure forSampling @08:34:12.90 SS.seek 25.00ml @08:34:14.30 Vacuum too high -- waiting up to 40s @08:34:14.78 Intake @38.7psia, vacuum from 20.0 to 24.0psi @08:34:14.79 SS.reconfigure forSampling @08:34:14.82 SS.seek 25.00ml @08:34:16.05 Vacuum too high -- waiting up to 40s @08:34:16.53 Intake @38.3psia, vacuum from 20.0 to 24.0psi @08:34:16.54 SS.reconfigure forSampling @08:34:16.57 SS.seek 25.00ml @08:34:17.77 Vacuum too high -- waiting up to 40s @08:34:18.47 Intake @39.1psia, vacuum from 20.0 to 24.0psi @08:34:18.48 SS.reconfigure forSampling @08:34:18.51 SS.seek 25.00ml @08:34:20.02 Vacuum too high -- waiting up to 40s @08:34:20.72 Intake @39.2psia, vacuum from 20.0 to 24.0psi @08:34:20.73 SS.reconfigure forSampling @08:34:20.76 SS.seek 25.00ml @08:34:22.24 Vacuum too high -- waiting up to 40s @08:34:22.72 Intake @37.9psia, vacuum from 20.0 to 24.0psi @08:34:22.73 SS.reconfigure forSampling @08:34:22.76 SS.seek 25.00ml @08:34:24.02 Vacuum too high -- waiting up to 40s @08:34:24.71 Intake @39.0psia, vacuum from 20.0 to 24.0psi @08:34:24.72 SS.reconfigure forSampling @08:34:24.75 SS.seek 25.00ml @08:34:26.05 Vacuum too high -- waiting up to 40s @08:34:26.53 Intake @38.3psia, vacuum from 20.0 to 24.0psi @08:34:26.54 SS.reconfigure forSampling @08:34:26.57 SS.seek 25.00ml @08:34:27.87 Vacuum too high -- waiting up to 40s @08:34:28.35 Intake @38.5psia, vacuum from 20.0 to 24.0psi @08:34:28.36 SS.reconfigure forSampling @08:34:28.39 SS.seek 25.00ml @08:34:29.46 Vacuum too high -- waiting up to 40s @08:34:30.34 Intake @38.8psia, vacuum from 20.0 to 24.0psi @08:34:30.35 SS.reconfigure forSampling @08:34:30.39 SS.seek 25.00ml @08:34:31.96 Vacuum too high -- waiting up to 40s @08:34:32.65 Intake @39.8psia, vacuum from 20.0 to 24.0psi @08:34:32.66 SS.reconfigure forSampling @08:34:32.70 SS.seek 25.00ml @08:34:34.24 Vacuum too high -- waiting up to 40s @08:34:34.94 Intake @39.0psia, vacuum from 20.0 to 24.0psi @08:34:34.95 SS.reconfigure forSampling @08:34:34.98 SS.seek 25.00ml @08:34:36.52 Vacuum too high -- waiting up to 40s @08:34:36.99 Intake @38.8psia, vacuum from 20.0 to 24.0psi @08:34:37.00 SS.reconfigure forSampling @08:34:37.04 SS.seek 25.00ml @08:34:38.15 Vacuum too high -- waiting up to 40s @08:34:38.62 Intake @38.8psia, vacuum from 20.0 to 24.0psi @08:34:38.63 SS.reconfigure forSampling @08:34:38.67 SS.seek 25.00ml @08:34:39.83 Vacuum too high -- waiting up to 40s @08:34:40.31 Intake @37.6psia, vacuum from 20.0 to 24.0psi @08:34:40.32 SS.reconfigure forSampling @08:34:40.35 SS.seek 25.00ml @08:34:41.68 Vacuum too high -- waiting up to 40s @08:34:42.37 Intake @38.1psia, vacuum from 20.0 to 24.0psi @08:34:42.38 SS.reconfigure forSampling @08:34:42.41 SS.seek 25.00ml @08:34:43.71 Vacuum too high -- waiting up to 40s @08:34:44.19 Intake @38.1psia, vacuum from 20.0 to 24.0psi @08:34:44.20 SS.reconfigure forSampling @08:34:44.23 SS.seek 25.00ml @08:34:45.46 Vacuum too high -- waiting up to 40s @08:34:46.14 Intake @38.5psia, vacuum from 20.0 to 24.0psi @08:34:46.15 SS.reconfigure forSampling @08:34:46.19 SS.seek 25.00ml @08:34:47.55 Vacuum too high -- waiting up to 40s @08:34:48.03 Intake @38.0psia, vacuum from 20.0 to 24.0psi @08:34:48.04 SS.reconfigure forSampling @08:34:48.08 SS.seek 25.00ml @08:34:49.24 Vacuum too high -- waiting up to 40s @08:34:49.92 Intake @38.2psia, vacuum from 20.0 to 24.0psi @08:34:49.93 SS.reconfigure forSampling @08:34:49.96 SS.seek 25.00ml @08:34:51.33 Vacuum too high -- waiting up to 40s @08:34:51.81 SS.seek 25.00ml @08:34:52.83 Vacuum too high -- waiting up to 40s @08:34:53.31 Intake @38.0psia, vacuum from 20.0 to 24.0psi @08:34:53.32 SS.reconfigure forSampling @08:34:53.35 SS.seek 25.00ml @08:34:54.46 Vacuum too high -- waiting up to 40s @08:34:54.93 Intake @38.3psia, vacuum from 20.0 to 24.0psi @08:34:54.94 SS.reconfigure forSampling @08:34:54.97 SS.seek 25.00ml @08:34:56.11 Vacuum too high -- waiting up to 40s @08:34:56.80 Intake @38.6psia, vacuum from 20.0 to 24.0psi @08:34:56.81 SS.reconfigure forSampling @08:34:56.84 SS.seek 25.00ml @08:34:58.18 Vacuum too high -- waiting up to 40s @08:34:58.66 Intake @38.2psia, vacuum from 20.0 to 24.0psi @08:34:58.67 SS.reconfigure forSampling @08:34:58.70 SS.seek 25.00ml @08:34:59.83 Vacuum too high -- waiting up to 40s @08:35:00.31 Intake @38.5psia, vacuum from 20.0 to 24.0psi @08:35:00.32 SS.reconfigure forSampling @08:35:00.35 SS.seek 25.00ml @08:35:01.33 Vacuum too high -- waiting up to 40s @08:35:01.80 Intake @38.5psia, vacuum from 20.0 to 24.0psi @08:35:01.81 SS.reconfigure forSampling @08:35:01.85 SS.seek 25.00ml @08:35:02.90 Vacuum too high -- waiting up to 40s @08:35:03.38 Intake @38.0psia, vacuum from 20.0 to 24.0psi @08:35:03.39 SS.reconfigure forSampling @08:35:03.42 SS.seek 25.00ml @08:35:05.74 Vacuum too high -- waiting up to 40s @08:35:06.45 Intake @39.3psia, vacuum from 20.0 to 24.0psi @08:35:06.46 SS.reconfigure forSampling @08:35:06.50 SS.seek 25.00ml @08:35:07.77 Vacuum too high -- waiting up to 40s @08:35:08.25 Intake @38.6psia, vacuum from 20.0 to 24.0psi @08:35:08.26 SS.reconfigure forSampling @08:35:08.30 SS.seek 25.00ml @08:35:09.36 Vacuum too high -- waiting up to 40s @08:35:09.84 Intake @38.3psia, vacuum from 20.0 to 24.0psi @08:35:09.85 SS.reconfigure forSampling @08:35:09.88 SS.seek 25.00ml @08:35:10.93 Vacuum too high -- waiting up to 40s @08:35:11.42 Intake @37.5psia, vacuum from 20.0 to 24.0psi @08:35:11.43 SS.reconfigure forSampling @08:35:11.46 SS.seek 25.00ml @08:35:12.55 Vacuum too high -- waiting up to 40s @08:35:13.03 Intake @38.6psia, vacuum from 20.0 to 24.0psi @08:35:13.04 SS.reconfigure forSampling @08:35:13.07 SS.seek 25.00ml @08:35:13.99 Vacuum too high -- waiting up to 40s @08:35:14.47 Intake @37.4psia, vacuum from 20.0 to 24.0psi @08:35:14.48 SS.reconfigure forSampling @08:35:14.52 SS.seek 25.00ml @08:35:15.64 Vacuum too high -- waiting up to 40s @08:35:16.12 Intake @38.3psia, vacuum from 20.0 to 24.0psi @08:35:16.13 SS.reconfigure forSampling @08:35:16.17 SS.seek 25.00ml @08:35:17.30 Vacuum too high -- waiting up to 40s @08:35:17.99 Intake @38.3psia, vacuum from 20.0 to 24.0psi @08:35:18.00 SS.reconfigure forSampling @08:35:18.03 SS.seek 25.00ml @08:35:19.30 Vacuum too high -- waiting up to 40s @08:35:19.77 Intake @38.1psia, vacuum from 20.0 to 24.0psi @08:35:19.78 SS.reconfigure forSampling @08:35:19.81 SS.seek 25.00ml @08:35:20.83 Vacuum too high -- waiting up to 40s @08:35:21.31 Intake @39.1psia, vacuum from 20.0 to 24.0psi @08:35:21.32 SS.reconfigure forSampling @08:35:21.36 SS.seek 25.00ml @08:35:22.43 Vacuum too high -- waiting up to 40s @08:35:22.90 Intake @39.0psia, vacuum from 20.0 to 24.0psi @08:35:22.91 SS.reconfigure forSampling @08:35:22.94 SS.seek 25.00ml @08:35:23.99 Vacuum too high -- waiting up to 40s @08:35:24.46 Intake @38.3psia, vacuum from 20.0 to 24.0psi @08:35:24.47 SS.reconfigure forSampling @08:35:24.51 SS.seek 25.00ml @08:35:25.49 Vacuum too high -- waiting up to 40s @08:35:25.96 Intake @38.4psia, vacuum from 20.0 to 24.0psi @08:35:25.97 SS.reconfigure forSampling @08:35:26.00 SS.seek 25.00ml @08:35:26.93 Vacuum too high -- waiting up to 40s @08:35:27.41 Intake @38.9psia, vacuum from 20.0 to 24.0psi @08:35:27.42 SS.reconfigure forSampling @08:35:27.45 SS.seek 25.00ml @08:35:28.52 Vacuum too high -- waiting up to 40s @08:35:29.27 Intake @39.5psia, vacuum from 20.0 to 24.0psi @08:35:29.28 SS.reconfigure forSampling @08:35:29.32 SS.seek 25.00ml @08:35:30.72 Vacuum too high -- waiting up to 40s @08:35:31.41 Intake @41.8psia, vacuum from 20.0 to 24.0psi @08:35:31.42 SS.reconfigure forSampling @08:35:31.45 SS.seek 25.00ml @08:35:32.47 Vacuum too high -- waiting up to 40s @08:35:32.73 Intake @38.7psia, vacuum from 20.0 to 24.0psi @08:35:32.74 SS.reconfigure forSampling @08:35:32.77 SS.seek 25.00ml @08:35:33.81 Vacuum too high -- waiting up to 40s @08:35:34.28 Intake @38.8psia, vacuum from 20.0 to 24.0psi @08:35:34.29 SS.reconfigure forSampling @08:35:34.32 SS.seek 25.00ml @08:35:35.40 Vacuum too high -- waiting up to 40s @08:35:35.87 Intake @38.8psia, vacuum from 20.0 to 24.0psi @08:35:35.88 SS.reconfigure forSampling @08:35:36.52 SS.seek 25.00ml @08:35:38.00 Vacuum too high -- waiting up to 40s @08:35:38.48 Intake @38.0psia, vacuum from 20.0 to 24.0psi @08:35:38.49 SS.reconfigure forSampling @08:35:38.53 SS.seek 25.00ml @08:35:39.65 Vacuum too high -- waiting up to 40s @08:35:40.13 Intake @38.5psia, vacuum from 20.0 to 24.0psi @08:35:40.14 SS.reconfigure forSampling @08:35:40.18 SS.seek 25.00ml @08:35:41.15 Vacuum too high -- waiting up to 40s @08:35:41.63 Intake @37.4psia, vacuum from 20.0 to 24.0psi @08:35:41.64 SS.reconfigure forSampling @08:35:41.68 SS.seek 25.00ml @08:35:42.84 Vacuum too high -- waiting up to 40s @08:35:43.32 Intake @38.6psia, vacuum from 20.0 to 24.0psi @08:35:43.33 SS.reconfigure forSampling @08:35:43.37 SS.seek 25.00ml @08:35:44.34 Vacuum too high -- waiting up to 40s @08:35:44.82 Intake @38.9psia, vacuum from 20.0 to 24.0psi @08:35:44.83 SS.reconfigure forSampling @08:35:44.87 SS.seek 25.00ml @08:35:45.84 Vacuum too high -- waiting up to 40s @08:35:46.32 Intake @38.3psia, vacuum from 20.0 to 24.0psi @08:35:46.33 SS.reconfigure forSampling @08:35:46.37 SS.seek 25.00ml @08:35:47.34 Vacuum too high -- waiting up to 40s @08:35:47.82 Intake @37.7psia, vacuum from 20.0 to 24.0psi @08:35:47.83 SS.reconfigure forSampling @08:35:47.87 SS.seek 25.00ml @08:35:48.84 Vacuum too high -- waiting up to 40s @08:35:49.32 Intake @37.6psia, vacuum from 20.0 to 24.0psi @08:35:49.33 SS.reconfigure forSampling @08:35:49.37 SS.seek 25.00ml @08:35:50.34 Vacuum too high -- waiting up to 40s @08:35:50.81 Intake @37.8psia, vacuum from 20.0 to 24.0psi @08:35:50.82 SS.reconfigure forSampling @08:35:50.85 SS.seek 25.00ml @08:35:51.90 Vacuum too high -- waiting up to 40s @08:35:52.37 Intake @38.0psia, vacuum from 20.0 to 24.0psi @08:35:52.38 SS.reconfigure forSampling @08:35:52.41 SS.seek 25.00ml @08:35:53.31 Vacuum too high -- waiting up to 40s @08:35:53.78 Intake @37.8psia, vacuum from 20.0 to 24.0psi @08:35:53.79 SS.reconfigure forSampling @08:35:53.83 SS.seek 25.00ml @08:35:54.84 Vacuum too high -- waiting up to 40s @08:35:55.31 Intake @37.4psia, vacuum from 20.0 to 24.0psi @08:35:55.32 SS.reconfigure forSampling @08:35:55.35 SS.seek 25.00ml @08:35:56.31 Vacuum too high -- waiting up to 40s @08:35:56.78 Intake @37.8psia, vacuum from 20.0 to 24.0psi @08:35:56.79 SS.reconfigure forSampling @08:35:56.83 SS.seek 25.00ml @08:35:57.81 Vacuum too high -- waiting up to 40s @08:35:58.28 Intake @38.7psia, vacuum from 20.0 to 24.0psi @08:35:58.29 SS.reconfigure forSampling @08:35:58.32 SS.seek 25.00ml @08:35:59.25 Vacuum too high -- waiting up to 40s @08:35:59.72 Intake @38.8psia, vacuum from 20.0 to 24.0psi @08:35:59.73 SS.reconfigure forSampling @08:35:59.76 SS.seek 25.00ml @08:36:00.78 Vacuum too high -- waiting up to 40s @08:36:01.27 Intake @38.7psia, vacuum from 20.0 to 24.0psi @08:36:01.28 SS.reconfigure forSampling @08:36:01.31 SS.seek 25.00ml @08:36:02.34 Vacuum too high -- waiting up to 40s @08:36:03.02 Intake @39.8psia, vacuum from 20.0 to 24.0psi @08:36:03.03 SS.reconfigure forSampling @08:36:03.06 SS.seek 25.00ml @08:36:03.21 SS.reconfigure SSconfig @08:36:03.24 SS.hold @08:36:03.36 Intake.select :close @08:36:03.50 ShallowSampler::Clogged in MAIN -- progress too slow after 322.8ml SSV.dial :exhaust @08:36:04.32 Exhaust.select :open @08:36:04.43 SS.reconfigure SSexhale @08:36:04.47 SS.seek 0.00ml @08:36:12.82 Exhaust.select :close @08:36:12.92 Sampled 322.8ml @08:36:12.93 collectSample: Complete Evacuating Puck (bubblePt=55.0psi, delay=15.0s) @08:36:12.94 Intake.select :close @08:36:13.60 CSV.dial :waste @08:36:13.64 CS.seek 0.000ml @08:36:13.72 SSV.dial CBV,avoiding => exhaust @08:36:13.74
CBV.dial SSV @08:36:13.78 CSV.dial :air @08:36:14.66 CS.seek 10.000ml @08:36:15.77
CTV.dial :air @08:36:15.91 Sleepy.power :sampler,:OFF @08:36:16.58
delay 15 seconds @08:36:31.58 SSV.dial :spit,avoiding => exhaust @08:36:32.98 CTV.dial CSV,avoiding => intake @08:36:33.00
SS.seek 0.00ml @08:36:33.10 SSV.dial CBV,avoiding => exhaust @08:36:40.69 CBV.dial between CSV and waste2b @08:36:41.68 CTV.dial CSV,avoiding => air @08:36:41.70
CSV.dial CTV @08:36:42.17 CS.reconfigure CSpressurize @08:36:42.20 CS.seek 0.000ml @08:37:06.57 BubblePoint: Intake @70.0psia, Outlet @14.9psia @08:37:06.58 SS.reconfigure SSconfig @08:37:06.61 SS.seek 10.00ml @08:37:12.58 delay 15 seconds @08:37:27.68 Evacuated: Intake @69.2psia, Outlet @-0.0psia @08:37:27.69 SSV.dial :spit,avoiding => exhaust @08:37:29.09 SS.reconfigure SSexhale @08:37:29.12 SS.seek 0.00ml @08:37:34.30 SS.reconfigure SSfast @08:37:34.34 SS.seek 5.00ml @08:37:37.56 SSV.dial CBV,avoiding => exhaust @08:37:37.58
CBV.dial SSV @08:37:39.01 CTV.dial :air,avoiding => intake @08:37:40.40 CSV.dial CTV @08:37:40.41
SS.reconfigure SSconfig @08:37:40.45 SS.seek 10.00ml @08:37:41.41 CSV.dial :air @08:37:41.95 CS.reconfigure CSfast @08:37:41.99 CS.seek 0.000ml @08:37:43.61
SSV.dial :spit,avoiding => exhaust @08:37:44.20 CS.coast @08:37:45.01
SS.reconfigure SSexhale @08:37:45.04 SS.seek 0.00ml @08:37:49.81 SS.reconfigure SSfast @08:37:49.84 SS.seek 5.00ml @08:37:52.74 SSV.dial CBV,avoiding => exhaust @08:37:52.76
CBV.dial SSV @08:37:55.14 SSV.dial :spit,avoiding => exhaust @08:37:56.54 SS.reconfigure SSconfig @08:37:56.57 SS.seek 0.00ml @08:37:59.78 SS.coast @08:37:59.81 Evacuating Puck (bubblePt=55.0psi, delay=15.0s) Intake.select :close @08:37:59.87 CSV.dial :waste @08:37:59.96
SSV.dial :exhaust @08:38:00.67 CS.seek 0.000ml @08:38:00.75
Exhaust.select :open @08:38:00.85 SS.reconfigure SSexhale @08:38:00.89 SS.seek 0.00ml @08:38:00.93 CSV.dial :air @08:38:01.30
Exhaust.select :close @08:38:01.40 SSV.dial CBV,avoiding => exhaust @08:38:01.42
CBV.dial SSV @08:38:02.01 CS.seek 10.000ml @08:38:03.43
CTV.dial :air @08:38:03.44 delay 15 seconds @08:38:07.44 Session Begun @08:38:18.44
SSV.dial :spit,avoiding => exhaust @08:38:19.85 CTV.dial CSV,avoiding => intake @08:38:19.87
SS.seek 0.00ml @08:38:19.98 SSV.dial CBV,avoiding => exhaust @08:38:27.41 CBV.dial between CSV and waste2b @08:38:28.41 CSV.dial between air and CTV @08:38:28.82 CS.seek 7.168ml @08:38:36.52 CTV.dial CSV,avoiding => air @08:38:36.53
CSV.dial CTV @08:38:36.87 CS.reconfigure CSpressurize @08:38:36.91 CS.seek 0.000ml @08:38:55.61 CSV.dial :air @08:38:56.16 CS.reconfigure CSfast @08:38:56.21 CS.seek 10.000ml @08:39:21.43 CSV.dial between air and CTV @08:39:21.84 CS.seek 2.179ml @08:39:41.90 CTV.dial CSV,avoiding => air @08:39:41.91
CSV.dial CTV @08:39:42.24 CS.reconfigure CSpressurize @08:39:42.29 CS.seek 0.000ml @08:39:44.34 BubblePoint: Intake @73.7psia, Outlet @17.8psia @08:39:44.35 SS.reconfigure SSconfig @08:39:44.41 SS.seek 10.00ml @08:39:50.26 delay 15 seconds @08:40:05.36 Evacuated: Intake @72.9psia, Outlet @-0.2psia SSV.dial :spit,avoiding => exhaust @08:40:07.33 SS.reconfigure SSexhale @08:40:07.38 SS.seek 0.00ml @08:40:12.59 SS.reconfigure SSfast @08:40:12.64 SS.seek 5.00ml @08:40:15.36 SSV.dial CBV,avoiding => exhaust @08:40:15.38
CBV.dial SSV @08:40:16.82 CTV.dial :air,avoiding => intake @08:40:18.22 CSV.dial CTV @08:40:18.24
SS.reconfigure SSconfig @08:40:18.29 SS.seek 10.00ml @08:40:19.23 CSV.dial :air @08:40:19.77 CS.reconfigure CSfast @08:40:19.82 CS.seek 0.000ml @08:40:21.46
SSV.dial :spit,avoiding => exhaust @08:40:22.91 SS.reconfigure SSexhale @08:40:22.95 SS.seek 0.00ml @08:40:24.42 CS.coast @08:40:27.98
SS.reconfigure SSfast @08:40:28.03 SS.seek 5.00ml @08:40:31.21 SSV.dial CBV,avoiding => exhaust @08:40:31.23
CBV.dial SSV @08:40:33.65 SSV.dial :spit,avoiding => exhaust @08:40:35.06 SS.reconfigure SSconfig @08:40:35.10 SS.seek 0.00ml @08:40:38.15 SS.coast @08:40:38.19 Lysing: Adding 1.5ml lysis @08:40:38.20 CBV.dial between CSV and waste1,avoiding => DBV @08:40:39.80 CTV.dial CSV @08:40:41.19 CSV.dial CSR @08:40:42.04 CSR.select :lysis @08:40:42.16 CS.reconfigure CSconfig @08:40:42.21 CS.seek 1.000ml @08:40:45.54 delay 5 seconds @08:40:50.55 CSR.select :air @08:40:50.71 CS.seek 5.999ml @08:41:04.16 delay 2 seconds @08:41:06.17 CSV.dial :waste1 @08:41:07.53 CS.reconfigure CSfast @08:41:07.57 CS.seek empty @08:41:23.04 CSV.dial CSR @08:41:24.40 CSR.select :lysis @08:41:24.51 CS.reconfigure CSconfig @08:41:24.56 CS.seek 1.500ml @08:41:29.29 delay 5 seconds @08:41:34.30 CSR.select :air @08:41:34.46 CS.seek 4.500ml @08:41:42.85 delay 2 seconds @08:41:44.86 CSV.dial CTV @08:41:45.41 CS.seek 1.275ml @08:41:54.36 Heating sh1 puck from tube 2 in Collection Clamp to 85.0C for 5:00 @08:41:54.37 CH.seek 85.0C,8:00 @08:43:43.69 Session Ended @08:44:44.68
CH.hold 5:00,85.0C @08:49:44.79 Collection Puck Heater at 84.9C after lysing @08:49:44.81 CS.seek 0.000ml @08:49:49.06 CTV.dial between spare and air,avoiding => intake @08:49:50.05 CSV.dial :air @08:49:50.50 CS.seek 5.000ml @08:50:03.92 CSV.dial CTV @08:50:04.44 delay 2 seconds @08:50:06.45 CSV.dial :air @08:50:06.97 delay 2 seconds @08:50:08.98 CSV.dial CTV @08:50:09.50 delay 2 seconds @08:50:11.51 CSV.dial :air @08:50:12.04 delay 2 seconds @08:50:14.04 CS.seek 0.000ml @08:50:27.55 Recovering Lysate into Collection syringe @08:50:27.56 CSV.dial CBV @08:50:28.55 CBV.dial CSV @08:50:28.91 CS.seek 5.000ml @08:50:42.39 delay 5 seconds @08:50:47.41 CTV.dial :air @08:50:47.89 CS.seek 7.001ml @08:50:53.80 delay 5 seconds @08:50:58.81 CSV.dial :air2 @08:50:59.24 CS.seek 10.000ml @08:51:07.67 CSV.dial between air and CTV,avoiding => CBV @08:51:08.80 Lysing Complete @08:51:08.85 Storing sh1 puck in Collection Clamp to tube 1 @08:51:08.97 SC.reconfigure SCtight @08:51:09.00 SC.seek 1 @08:51:11.24 SC.reconfigure SCloose @08:51:11.41 Elbow.reconfigure TightElbow @08:51:11.44 Elbow.seek 1 @08:51:13.09 Forearm.reconfigure TightForearm @08:51:13.12 Forearm.seek 1 + 800 counts @08:51:14.12 SE.reconfigure SEpick @08:51:14.17 SE.seek beamBreak @08:51:15.73 SE.configure SEconfig @08:51:15.85 Elbow.reconfigure LimpElbow @08:51:15.88 Forearm.reconfigure LooseForearm @08:51:15.92 Forearm.seek 1 @08:51:16.12 Elbow.hold @08:51:16.18 Elbow.reconfigure TightElbow @08:51:16.25 SE.seek 14.05 Pucks below Top Plate @08:51:16.67 Forearm.coast @08:51:16.70 Forearm.seek 1 + 321 counts @08:51:16.94 Forearm.reconfigure TightForearm @08:51:16.97 Forearm.seek 1 + 800 counts @08:51:17.24 SE.seek 15.05 Pucks below Top Plate @08:51:17.79 Forearm.reconfigure LooseForearm @08:51:17.82 Forearm.seek retract @08:51:19.38 Elbow.seek CC @08:51:20.97 Elbow.reconfigure LooseElbow @08:51:21.06 Forearm.seek CC @08:51:22.02 CC.seek sealed @08:51:22.95 Hand.grip @08:51:23.72 CC.seek open @08:51:27.06 Forearm.seek retract @08:51:27.99 Elbow.reconfigure TightElbow @08:51:28.03 Elbow.seek 1 @08:51:29.63 Elbow.reconfigure LimpElbow @08:51:29.68 Forearm.seek 1 @08:51:30.68 Elbow.hold @08:51:30.73 Elbow.reconfigure TightElbow @08:51:30.77 Hand.release @08:51:31.55 Forearm.coast @08:51:31.58 Forearm.seek 1 + 341 counts @08:51:31.86 Forearm.reconfigure TightForearm @08:51:31.89 Forearm.seek 1 + 800 counts @08:51:32.22 SE.seek down @08:51:33.33
Forearm.reconfigure LooseForearm @08:51:33.36 Forearm.seek retract @08:51:33.50 SE.coast @08:51:34.28
SC.coast @08:51:34.31 Tranferring lysate to PRV @08:51:34.32 CSV.dial :air @08:51:34.63 CS.seek 10.000ml @08:51:34.70 CSV.dial between air and CTV @08:51:35.08 AIV.dial :bypass @08:51:35.24 Beginning samplePassC @08:51:35.25 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:51:35.39 Elbow.seek FlushPuck::Garage @08:51:37.14 Elbow.reconfigure LooseElbow @08:51:37.22 Forearm.seek garage @08:51:38.21 Hand.grip @08:51:39.06 Forearm.seek retract @08:51:40.06 Elbow.reconfigure TightElbow @08:51:40.10 Elbow.seek CC @08:51:42.84 Elbow.reconfigure LooseElbow @08:51:42.92 Forearm.reconfigure TightForearm @08:51:42.95 Forearm.seek CC @08:51:44.07 CC.seek closed @08:51:47.91 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 2.10 @08:51:47.97 Hand.release @08:51:48.76 Forearm.reconfigure LooseForearm @08:51:48.79 Forearm.seek retract @08:51:49.76 AWV.select :air @08:51:49.77 AIV.dial :bypass @08:51:49.78 PSV.dial PSR @08:51:51.08 PSR.select :flush @08:51:51.19 PS.seek 1.500ml @08:52:07.25 delay 5 seconds @08:52:12.26 PSV.dial :waste1 @08:52:12.84 PS.seek 0.000ml @08:52:28.93 PSV.dial PSR @08:52:29.36 PSR.select :air @08:52:29.46 PS.seek 2.500ml @08:52:55.67 CBV.dial PRV @08:52:55.68 PRV.dial CBV @08:52:56.46
PSV.dial :mixloop @08:52:57.57 CTV.dial :air,avoiding => intake @08:52:57.58 PTV.dial :PRVmixing @08:52:57.66 PS.seek 0.000ml @08:53:23.94 PSV.dial :air @08:53:24.41 PS.seek 2.500ml @08:53:50.72 PSV.dial :mixloop @08:53:51.19 PS.seek 0.000ml @08:54:17.47 PSV.dial :air @08:54:17.93 PS.seek 2.500ml @08:54:44.19 PSV.dial :mixloop @08:54:44.65 PS.seek 0.000ml @08:55:10.85 Garaging FlushPuck in Collection Clamp @08:55:10.91 Elbow.reconfigure TightElbow @08:55:10.94 Elbow.seek CC @08:55:11.04 Elbow.reconfigure LooseElbow @08:55:11.13 Forearm.seek CC @08:55:12.11 CC.seek sealed @08:55:13.01 Hand.grip @08:55:13.72 CC.seek open @08:55:17.05 Forearm.seek retract @08:55:17.99 Elbow.reconfigure TightElbow @08:55:18.03 Elbow.seek garage @08:55:20.80 Elbow.reconfigure LooseElbow @08:55:20.89 Forearm.reconfigure TightForearm @08:55:20.92 Forearm.seek garage @08:55:22.09 Hand.release @08:55:22.89 Forearm.reconfigure LooseForearm @08:55:22.92 Forearm.seek retract @08:55:23.95 Connecting PS to CS to transfer sample @08:55:23.96 CBV.dial AIV @08:55:23.97 AIV.dial :bypass @08:55:23.98 PSV.dial :mixloop @08:55:23.99 PTV.dial :PRVmixing @08:55:24.00 PRV.dial AIV @08:55:24.01 CTV.dial :air,avoiding => intake @08:55:24.02 Loading lyfil puck from tube 2 into Collection Clamp @08:55:24.19 SC.reconfigure SCtight @08:55:24.22 SC.seek 2 @08:55:26.45 SC.reconfigure SCloose @08:55:26.62 Elbow.reconfigure TightElbow @08:55:26.66 Elbow.seek 2 @08:55:28.50 Forearm.reconfigure TightForearm @08:55:28.53 Forearm.seek 2 + 800 counts @08:55:29.62 SE.reconfigure SEpick @08:55:29.67 SE.seek beamBreak @08:55:32.34 SE.configure SEconfig @08:55:32.46 Elbow.seek 2 @08:55:32.60 Forearm.seek 2 + 300 counts @08:55:32.87 Forearm.reconfigure LooseForearm @08:55:32.91 Hand.grip @08:55:33.61 SE.seek down @08:55:33.75
Forearm.seek retract @08:55:34.71 Elbow.seek CC @08:55:35.88 SE.coast @08:55:36.23
Elbow.reconfigure LooseElbow @08:55:36.31 Forearm.reconfigure TightForearm @08:55:36.34 Forearm.seek CC @08:55:37.46 CC.seek closed @08:55:41.21 Collection Clamp onSpring/offSpring for 5/22 samples, on/off torque 2.00 @08:55:41.22 Hand.release @08:55:42.01 Forearm.reconfigure LooseForearm @08:55:42.04 Forearm.seek retract @08:55:42.96 SC.coast @08:55:42.99 CTV.dial CSV,avoiding => intake @08:55:44.28 CSV.dial CTV @08:55:44.57 CS.seek 5.000ml @08:55:58.04 delay 1:20 @08:57:18.05 CS.seek 2.500ml @08:57:25.19 delay 40 seconds @08:58:05.20 CS.seek 0.000ml @08:58:12.32 delay 1:00 @08:59:12.33 PSV.dial :air @08:59:12.80 PS.seek 2.500ml @08:59:39.12 PSV.dial :mixloop @08:59:39.57 delay 3 seconds @08:59:42.58 PSV.dial :air @08:59:43.04 delay 2 seconds @08:59:45.05 PSV.dial :mixloop @08:59:45.51 delay 3 seconds @08:59:48.52 PSV.dial :waste1 @08:59:49.81 PS.seek empty @09:00:16.12 CBV.dial :waste1 @09:00:16.58 delay 5 seconds @09:00:21.59 CBV.dial AIV @09:00:22.05 delay 10 seconds @09:00:32.06 CBV.dial :waste1 @09:00:32.53 delay 10 seconds @09:00:42.54 CBV.dial AIV @09:00:43.01 delay 10 seconds @09:00:53.02 CTV.dial CSV,avoiding => intake @09:00:53.03 CBV.dial between AIV and waste1 @09:00:53.89 CSV.dial :air @09:00:54.40 CS.seek 10.000ml @09:01:20.47 CSV.dial CTV @09:01:20.98 CS.seek 0.000ml @09:01:47.21 CBV.dial :waste1 @09:01:47.51 delay 10 seconds @09:01:57.52 CTV.dial :air,avoiding => intake @09:01:58.87 Storing lyfil puck in Collection Clamp to tube 1 @09:01:59.00 SC.reconfigure SCtight @09:01:59.03 SC.seek 1 @09:02:01.22 SC.reconfigure SCloose @09:02:01.40 Elbow.reconfigure TightElbow @09:02:01.43 Elbow.seek 1 @09:02:03.03 Forearm.reconfigure TightForearm @09:02:03.06 Forearm.seek 1 + 800 counts @09:02:04.12 SE.reconfigure SEpick @09:02:04.17 SE.seek beamBreak @09:02:05.65 SE.configure SEconfig @09:02:05.77 Elbow.reconfigure LimpElbow @09:02:05.81 Forearm.reconfigure LooseForearm @09:02:05.85 Forearm.seek 1 @09:02:06.06 Elbow.hold @09:02:06.12 Elbow.reconfigure TightElbow @09:02:06.21 SE.seek 15.05 Pucks below Top Plate @09:02:06.61 Forearm.coast @09:02:06.64 Forearm.seek 1 + 338 counts @09:02:06.87 Forearm.reconfigure TightForearm @09:02:06.90 Forearm.seek 1 + 800 counts @09:02:07.13 SE.seek 16.05 Pucks below Top Plate @09:02:07.66 Forearm.reconfigure LooseForearm @09:02:07.69 Forearm.seek retract @09:02:08.61 Elbow.seek CC @09:02:10.15 Elbow.reconfigure LooseElbow @09:02:10.23 Forearm.seek CC @09:02:11.21 CC.seek sealed @09:02:12.15 Hand.grip @09:02:12.83 CC.seek open @09:02:16.17 Forearm.seek retract @09:02:17.12 Elbow.reconfigure TightElbow @09:02:17.16 Elbow.seek 1 @09:02:18.78 Elbow.reconfigure LimpElbow @09:02:18.82 Forearm.seek 1 @09:02:19.85 Elbow.hold @09:02:19.90 Elbow.reconfigure TightElbow @09:02:19.94 Hand.release @09:02:20.81 Forearm.coast @09:02:20.84 Forearm.seek 1 + 330 counts @09:02:21.08 Forearm.reconfigure TightForearm @09:02:21.11 Forearm.seek 1 + 800 counts @09:02:21.33 SE.seek down @09:02:22.42
Forearm.reconfigure LooseForearm @09:02:22.47 Forearm.seek retract @09:02:22.58 SE.coast @09:02:23.38
SC.coast @09:02:23.41 PSV.dial :air @09:02:24.48 PS.seek 0.499ml @09:02:30.45 delay 3 seconds @09:02:33.46 PSV.dial :mixloop @09:02:33.91 PRV.dial :air @09:02:34.37 delay 3 seconds @09:02:37.38 PRV.dial CBV @09:02:37.83 CBV.dial AIV @09:02:38.29 PRV.dial between CBV and air @09:02:38.61 PRV.dial :air @09:02:38.96 PS.reconfigure PSslow @09:02:38.99 PS.seek 0.599ml @09:02:41.45 PRV.dial :waste2 @09:02:42.23 PS.reconfigure PSconfig @09:02:42.27 PS.seek empty @09:02:49.33 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @09:02:49.48 Elbow.seek FlushPuck::Garage @09:02:51.23 Elbow.reconfigure LooseElbow @09:02:51.32 Forearm.seek garage @09:02:52.37 Hand.grip @09:02:53.72 Forearm.seek retract @09:02:54.75 Elbow.reconfigure TightElbow @09:02:54.78 Elbow.seek CC @09:02:57.56 Elbow.reconfigure LooseElbow @09:02:57.64 Forearm.reconfigure TightForearm @09:02:57.67 Forearm.seek CC @09:02:58.84 CC.seek closed @09:03:02.65 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 2.00 @09:03:02.70 Hand.release @09:03:03.49 Forearm.reconfigure LooseForearm @09:03:03.52 Forearm.seek retract @09:03:04.54 Clearing PML for modified lysate @09:03:04.55 CTV.dial :air,avoiding => intake @09:03:04.56 PRV.dial :waste2 @09:03:04.57 PTV.dial :PRV,avoiding => puck @09:03:05.29 delay 2 seconds @09:03:07.29 PTV.dial :PRVmixing,avoiding => puck @09:03:08.03 delay 2 seconds @09:03:10.04 PSV.dial :PML @09:03:10.05 delay 2 seconds @09:03:12.05 PRV.dial :air @09:03:12.78 delay 2 seconds @09:03:14.84 PS.seek 1.499ml @09:03:30.98 delay 5 seconds @09:03:35.99 PS.seek 2.500ml @09:03:46.95 PSV.dial :air @09:03:47.40 PS.seek 0.000ml @09:04:13.64 mixing lysate with speDil @09:04:13.65 PSV.dial :PML @09:04:14.09 CBV.dial :PRV @09:04:14.10 PRV.dial :air @09:04:14.11 delay 2 seconds @09:04:16.12 PRV.dial :speDil @09:04:16.56 delay 2 seconds @09:04:18.62 PS.reconfigure PSslow1 @09:04:18.65 PS.seek 0.225ml @09:04:35.59 Session Begun @09:04:42.17
delay 5 seconds @09:04:45.10 -> showlog @09:04:47.18
PRV.dial CBV @09:04:47.92 delay 2 seconds @09:04:49.98 PS.seek 0.475ml @09:05:15.79 delay 5 seconds @09:05:20.80 PRV.dial :air @09:05:21.28 delay 2 seconds @09:05:23.29 PS.seek 1.000ml @09:06:17.38 delay 10 seconds @09:06:27.39 PRV.dial CBV @09:06:27.85 CTV.dial :air,avoiding => intake @09:06:27.86 PSV.dial :air @09:06:28.35 PS.reconfigure PSconfig @09:06:28.39 PS.seek 2.500ml @09:06:44.51 delay 3 seconds @09:06:47.52 PSV.dial PTV @09:06:48.27 PTV.dial :PRV,avoiding => puck @09:06:49.03 PS.seek 0.000ml @09:07:15.29 PSV.dial :air @09:07:16.05 PS.seek 2.500ml @09:07:42.35 delay 3 seconds @09:07:45.36 PSV.dial PTV @09:07:46.22 PTV.dial :PRV,avoiding => puck @09:07:46.24 PS.seek 0.000ml @09:08:12.54 Pushing modified lysate into AIV Injection Loop @09:08:12.55 CBV.dial AIV @09:08:12.57 AIV.dial :bypass @09:08:12.59 PRV.dial CBV @09:08:12.61 PSV.dial :air @09:08:13.34 PS.seek 2.500ml @09:08:39.66 delay 3 seconds @09:08:42.67 PSV.dial :PML @09:08:43.13 PTV.dial :PRVmixing,avoiding => puck @09:08:43.93 PS.reconfigure PSslow1 @09:08:43.98 PS.seek 1.740ml @09:08:48.32 Session Ended @09:10:01.67
delay 5 seconds @09:10:06.68 AIV.dial :sample @09:10:07.20 AWV.select :waste @09:10:07.35 PS.reconfigure PSslow2 @09:10:07.38 PS.seek 1.220ml @09:11:53.38 delay 15 seconds @09:12:08.39 AIV.dial :bypass @09:12:08.94 PS.reconfigure PSconfig @09:12:08.97 PS.seek 0.000ml @09:12:22.19 PSV.dial :air @09:12:22.64 PS.seek 2.500ml @09:12:48.88 PSV.dial :PML @09:12:49.33 PS.seek 0.000ml @09:13:15.60 AIV.dial :coilToMFB @09:13:15.61 AWV.select :air @09:13:15.75 Extracting Nucleic Acid from AIV @09:13:15.77
Beginning samplePassD @09:13:16.37 SPE.hold 2:46:40,55.0C @09:13:16.39 ASV.dial :SPEdelivery @09:13:16.52
AWV.select :air @09:13:16.53 AIV.dial :bypass @09:13:16.54 PSV.dial PSR @09:13:16.94 delay 20 seconds @09:13:17.57
PSR.select :killMFB @09:13:17.67 PS.seek 1.500ml @09:13:33.72 delay 5 seconds @09:13:36.95 primeLysateINT @09:13:36.96 AWV.select :air @09:13:36.97 AIV.dial :coilToMFB @09:13:36.98 ATV.dial :AIV @09:13:38.33 AS.reconfigure ASslow1 @09:13:38.37 AS.seek 0.241ml @09:13:38.73
PSV.dial :waste1 @09:13:39.18 PS.seek 0.000ml @09:13:55.22 PSV.dial PSR @09:13:55.67 PSR.select :air @09:13:55.77 PS.seek 2.500ml @09:14:22.09 PRV.dial CBV @09:14:22.11
CBV.dial PRV @09:14:22.12 PSV.dial :mixloop @09:14:23.16 CTV.dial :air,avoiding => intake @09:14:23.17 PTV.dial :PRVmixing @09:14:23.19 PS.seek 0.000ml @09:14:28.01 delay 10 seconds @09:14:38.02 ATV.dial :air2 @09:14:38.61 AS.seek 0.271ml @09:14:44.97 ATV.dial :waste2 @09:14:45.35 AS.reconfigure ASnorm @09:14:45.38 AS.seek empty @09:14:49.41
PSV.dial PSR @09:14:50.56 PSR.select :flush @09:14:50.66 PS.seek 1.500ml @09:14:59.67 Lysate primed! @09:14:59.68 AFV.select :holdingCoil @09:14:59.69 ATV.dial :air2 @09:15:00.09 AS.reconfigure ASslow1 @09:15:00.12 AS.seek 0.069ml @09:15:06.81
delay 5 seconds @09:15:11.82 PSV.dial :waste1 @09:15:12.29 PS.seek 0.000ml @09:15:14.68 delay 3 seconds @09:15:17.69 ATV.dial :AIV @09:15:18.26 AS.seek 0.469ml @09:15:28.44
PSV.dial PSR @09:15:28.90 PSR.select :air @09:15:29.00 PS.seek 2.500ml @09:15:55.18 PRV.dial CBV @09:15:55.20
CBV.dial PRV @09:15:55.21 PSV.dial :mixloop @09:15:56.23 CTV.dial :air,avoiding => intake @09:15:56.24 PTV.dial :PRVmixing @09:15:56.26 PS.seek 0.000ml @09:16:22.50 PSV.dial PSR @09:16:23.64 PSR.select :flush @09:16:23.74 PS.seek 1.500ml @09:16:39.84 delay 5 seconds @09:16:40.07 delay 5 seconds @09:16:44.85
PSV.dial :waste1 @09:16:45.12 Solid Phase Extraction Heater at 51.8C @09:16:45.13 ATV.dial ASV @09:16:45.32
PS.seek 0.000ml @09:16:45.48 ASV.dial :SPEdelivery @09:16:45.54 AS.seek 0.041ml @09:17:01.40
PSV.dial PSR @09:17:01.85 PSR.select :air @09:17:01.95 PS.seek 2.500ml @09:17:28.24 PRV.dial CBV @09:17:28.26
CBV.dial PRV @09:17:28.27 PSV.dial :mixloop @09:17:29.28 CTV.dial :air,avoiding => intake @09:17:29.29 PTV.dial :PRVmixing @09:17:29.31 PS.seek 0.000ml @09:17:55.56 PSV.dial PSR @09:17:56.57 PSR.select :flush @09:17:56.67 PS.seek 1.500ml @09:18:12.71 delay 5 seconds @09:18:13.07 delay 10 seconds @09:18:17.72
PSV.dial :waste1 @09:18:18.17 PS.seek 0.000ml @09:18:23.08 rinseLysateLineINT @09:18:23.09 AFV.select :carrierSolution @09:18:23.18 delay 2 seconds @09:18:25.19 AS.reconfigure ASconfig @09:18:25.22 AS.seek 1.000ml @09:18:34.21
PSV.dial PSR @09:18:34.66 PSR.select :air @09:18:34.76 PS.seek 2.500ml @09:19:01.08 PRV.dial CBV @09:19:01.10
CBV.dial PRV @09:19:01.11 PSV.dial :mixloop @09:19:02.12 CTV.dial :air,avoiding => intake @09:19:02.13 PTV.dial :PRVmixing @09:19:02.14 PS.reconfigure PSslow @09:19:02.18 PS.seek 1.200ml @09:19:21.38 delay 1 second @09:19:22.39 AFV.select :holdingCoil @09:19:22.48 delay 1 second @09:19:23.49 ATV.dial :AIV @09:19:23.83 AIV.dial :coilToMFB @09:19:23.84 AWV.select :waste @09:19:23.93 AS.reconfigure ASslow0 @09:19:23.97 AS.seek 0.000ml @09:19:29.08
PSV.dial :waste1 @09:19:30.39 PS.reconfigure PSconfig @09:19:30.42 PS.seek 0.000ml @09:19:43.49 PSV.dial :air @09:19:44.51 PS.seek 2.500ml @09:20:10.80 PSV.dial :mixloop @09:20:11.25 PS.reconfigure PSslow @09:20:11.82 PS.seek 0.000ml @09:21:03.14 PSV.dial :air @09:21:03.71 PS.reconfigure PSconfig @09:21:03.74 PS.seek 2.500ml @09:21:06.31 ATV.dial :air2 @09:21:06.99 AS.reconfigure ASslow1 @09:21:07.02 AS.seek 0.050ml @09:21:17.53 ATV.dial :AIV @09:21:18.10 AS.seek 0.010ml @09:21:26.50 ATV.dial :waste @09:21:27.79 AS.seek empty @09:21:29.98
PSV.dial :mixloop @09:21:30.27 ethanol wash @09:21:30.28 ATV.dial :air2 @09:21:30.42
PS.reconfigure PSslow @09:21:30.45 PS.seek 0.000ml @09:21:31.23 AS.reconfigure ASslow2 @09:21:31.26 AS.seek 0.009ml @09:21:41.84 delay 3 seconds @09:21:44.85 Pulling 0.4ml of Ethanol Wash @09:21:44.86 ATV.dial :ethanol @09:21:45.52 AS.reconfigure ASslow1 @09:21:45.55 AS.seek 0.409ml @09:22:21.73
Cleaning Collection Syringe @09:22:21.74 CSV.dial CSR @09:22:22.17 CSR.select :flush @09:22:22.27 CS.seek 4.000ml @09:22:33.20 delay 5 seconds @09:22:38.21 CSR.select :air @09:22:38.30 CS.seek 10.000ml @09:22:54.26 CSV.dial CTV @09:22:54.28
CTV.dial CSV @09:22:55.62 CBV.dial :waste1 @09:22:56.10 CS.seek 0.000ml @09:23:07.45 delay 3 seconds @09:23:10.46 ATV.dial :air1 @09:23:10.79 delay 3 seconds @09:23:13.85 AS.seek 0.434ml @09:23:19.20 delay 3 seconds @09:23:22.21 ATV.dial :ethanol @09:23:22.26
CSV.dial CSR @09:23:22.59 AS.seek 0.419ml @09:23:22.96
CSR.select :flush @09:23:23.06 CS.seek 4.000ml @09:23:25.90 delay 3 seconds @09:23:28.91 ATV.dial ASV @09:23:30.05 AS.seek 0.092ml @09:23:34.04
delay 5 seconds @09:23:39.05 CSR.select :air @09:23:39.14 CS.seek 10.000ml @09:23:55.20 CSV.dial CTV @09:23:55.22
CTV.dial CSV @09:23:55.65 CBV.dial :waste1 @09:23:55.67 CS.seek 0.000ml @09:24:21.84 Garaging FlushPuck in Collection Clamp @09:24:21.89 Elbow.reconfigure TightElbow @09:24:21.93 Elbow.seek CC @09:24:22.03 Elbow.reconfigure LooseElbow @09:24:22.11 Forearm.seek CC @09:24:23.10 CC.seek sealed @09:24:24.01 Hand.grip @09:24:24.78 CC.seek open @09:24:28.10 Forearm.seek retract @09:24:29.04 Elbow.reconfigure TightElbow @09:24:29.08 Elbow.seek garage @09:24:31.90 Elbow.reconfigure LooseElbow @09:24:31.98 Forearm.reconfigure TightForearm @09:24:32.01 Forearm.seek garage @09:24:33.16 Hand.release @09:24:33.95 Forearm.reconfigure LooseForearm @09:24:33.98 Forearm.seek retract @09:24:34.98 Whole Cell Archive sampling 1000ml @09:24:35.00 Loading wcr puck from tube 2 into Collection Clamp @09:24:35.18 SC.reconfigure SCtight @09:24:35.22 SC.seek 2 @09:24:36.95 delay 3 seconds @09:24:37.39 SC.reconfigure SCloose @09:24:37.57 Elbow.reconfigure TightElbow @09:24:37.60 Elbow.seek 2 @09:24:39.47 Forearm.reconfigure TightForearm @09:24:39.50 Forearm.seek 2 + 800 counts @09:24:39.96 ATV.dial :waste2 @09:24:40.50 AS.reconfigure ASconfig @09:24:40.53 AS.seek empty @09:24:40.65 SE.reconfigure SEpick @09:24:40.71 SE.seek beamBreak @09:24:43.30 SE.configure SEconfig @09:24:44.02 Elbow.seek 2 @09:24:44.19 Forearm.seek 2 + 300 counts @09:24:44.46 Forearm.reconfigure LooseForearm @09:24:44.50 Hand.grip @09:24:45.30 SE.seek down @09:24:45.45 Forearm.seek retract @09:24:46.23 ATV.dial :air2 @09:24:46.45 Elbow.seek CC @09:24:46.57 AS.reconfigure ASslow1 @09:24:46.60 AS.seek 1.000ml @09:24:47.67 SE.coast @09:24:48.07 Elbow.reconfigure LooseElbow @09:24:48.16 Forearm.reconfigure TightForearm @09:24:48.19 Forearm.seek CC @09:24:49.31 CC.seek closed @09:24:53.13 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.98 @09:24:53.14 Hand.release @09:24:53.95 Forearm.reconfigure LooseForearm @09:24:53.98 Forearm.seek retract @09:24:54.96 SC.coast @09:24:54.99 collectSample 1000ml w/bubble point of 55psi @09:24:55.01 Priming 7ml Sleepy.power :sampler,:ON @09:24:55.02 Intake.select :close @09:24:55.03 Exhaust.select :close @09:24:55.04 SS.hold @09:24:55.19 Calibrating pressure sensors @09:24:55.20 CTV.dial :air @09:24:56.50 SSV.dial :spit,avoiding => exhaust @09:24:56.51 SS.reconfigure SSfast @09:24:56.54 SS.seek 5.00ml @09:24:59.25 SSV.dial CBV @09:24:59.26 CBV.dial SSV @09:25:00.66 delay 3.8 seconds @09:25:04.55 SSV.dial :spit @09:25:05.89 SS.seek 0.00ml @09:25:08.55 SSV.dial CBV @09:25:09.88 CTV.dial :intake @09:25:10.60 Waiting for external sampler to finish priming... @09:25:10.61 delayUntil "09:34:55.01" @09:28:11.01 delay 5 seconds @09:28:16.02 ATV.dial ASV @09:28:16.73 ASV.dial :SPEdelivery @09:28:16.75 AS.seek 0.020ml @09:31:36.91 delay 5 seconds @09:31:41.92 ATV.dial :air2 @09:31:42.68 AS.seek 0.520ml @09:33:24.91 delay 5 seconds @09:33:29.92 ATV.dial ASV @09:33:30.64 ASV.dial :wasteR @09:33:31.25 AS.seek 0.300ml @09:34:16.38 delay 20 seconds @09:34:36.39 AS.seek 0.070ml @09:34:55.02 SSV.dial CBV @09:34:55.04 CBV.dial SSV @09:34:55.05 Allowing 55s for 7.0ml of remaining 7.0ml @09:34:55.06 Intake.select :open @09:34:57.19 Intake @42.7psia, vacuum from 20.0 to 24.0psi @09:34:57.21 SS.reconfigure forSampling @09:34:57.24 SS.seek 7.00ml @09:34:59.82 Vacuum too high -- waiting up to 40s @09:35:00.75 Intake @42.7psia, vacuum from 20.0 to 24.0psi @09:35:00.76 SS.reconfigure forSampling @09:35:00.80 SS.seek 7.00ml @09:35:03.22 Vacuum too high -- waiting up to 40s @09:35:04.15 Intake @39.9psia, vacuum from 20.0 to 24.0psi @09:35:04.17 SS.reconfigure forSampling @09:35:04.20 SS.seek 7.00ml @09:35:06.33 Stroke completed -- waiting for pressure to equalize ... @09:35:09.90 Intake.select :close @09:35:10.03 SSV.dial :exhaust @09:35:10.81 Exhaust.select :open @09:35:10.91 SS.reconfigure SSexhale @09:35:11.57 SS.seek 0.00ml @09:35:15.12 Exhaust.select :close @09:35:15.23 Sampling 1000.0ml @09:35:15.24 Intake.select :close @09:35:15.25 Exhaust.select :close @09:35:15.26 SS.reconfigure SSconfig @09:35:15.30 SS.hold @09:35:15.47 CTV.dial :intake @09:35:15.49 SSV.dial CBV @09:35:15.51 CBV.dial SSV @09:35:16.30 Allowing 185s for 25.0ml of remaining 1000.0ml @09:35:16.31 Intake.select :open @09:35:18.45 Intake @43.4psia, vacuum from 20.0 to 24.0psi @09:35:18.46 SS.reconfigure forSampling @09:35:18.49 SS.seek 25.00ml @09:35:20.66 Vacuum too high -- waiting up to 40s @09:35:20.93 Intake @39.7psia, vacuum from 20.0 to 24.0psi @09:35:20.95 SS.reconfigure forSampling @09:35:20.98 SS.seek 25.00ml @09:35:23.19 Vacuum too high -- waiting up to 40s @09:35:23.53 delay 20 seconds @09:35:23.90 Intake @41.5psia, vacuum from 20.0 to 24.0psi @09:35:23.92 SS.reconfigure forSampling @09:35:23.95 SS.seek 25.00ml @09:35:26.16 Vacuum too high -- waiting up to 40s @09:35:26.65 Intake @43.2psia, vacuum from 20.0 to 24.0psi @09:35:26.67 SS.reconfigure forSampling @09:35:26.70 SS.seek 25.00ml @09:35:29.97 Vacuum too high -- waiting up to 40s @09:35:30.23 Intake @39.9psia, vacuum from 20.0 to 24.0psi @09:35:30.24 SS.reconfigure forSampling @09:35:30.28 SS.seek 25.00ml @09:35:43.54 ATV.dial :waste2 @09:35:44.18 AS.reconfigure ASconfig @09:35:44.21 AS.seek empty @09:35:48.62 Elution @09:35:48.63 ATV.dial :air1 @09:35:49.60 AS.reconfigure ASslow1 @09:35:49.63 AS.seek 0.049ml @09:35:49.95 Stroke completed -- waiting for pressure to equalize ... @09:35:50.59 Intake.select :close @09:35:50.73 SSV.dial :exhaust @09:35:51.51 Exhaust.select :open @09:35:51.61 SS.reconfigure SSexhale @09:35:51.65 SS.seek 0.00ml @09:36:00.14 delay 3 seconds @09:36:00.40 Exhaust.select :close @09:36:00.50 SSV.dial CBV @09:36:00.52 CBV.dial SSV @09:36:01.30 Allowing 185s for 25.0ml of remaining 975.0ml @09:36:01.31 Intake.select :open @09:36:03.15 ATV.dial :ElutionWater @09:36:03.44 Intake @43.4psia, vacuum from 20.0 to 24.0psi @09:36:03.46 SS.reconfigure forSampling @09:36:03.49 SS.seek 25.00ml @09:36:03.72 AS.seek 0.109ml @09:36:16.22 Vacuum too high -- waiting up to 40s @09:36:16.25 delay 3 seconds @09:36:16.49 Intake @40.6psia, vacuum from 20.0 to 24.0psi @09:36:16.51 SS.reconfigure forSampling @09:36:16.54 SS.seek 25.00ml @09:36:19.26 ATV.dial ASV,via => air2 @09:36:20.92 ASV.dial :SPEelution @09:36:21.70 AFV.select :carrierSolution @09:36:21.85 AS.reconfigure ASnorm @09:36:21.88 AS.seek 0.759ml @09:36:40.48 Stroke completed -- waiting for pressure to equalize ... @09:36:41.12 Intake.select :close @09:36:41.26 SSV.dial :exhaust @09:36:42.02 Exhaust.select :open @09:36:42.12 SS.reconfigure SSexhale @09:36:42.16 SS.seek 0.00ml @09:36:51.34 Exhaust.select :close @09:36:51.44 SSV.dial CBV @09:36:51.46 CBV.dial SSV @09:36:52.24 Allowing 185s for 25.0ml of remaining 950.0ml @09:36:52.26 Intake.select :open @09:36:54.39 Intake @44.5psia, vacuum from 20.0 to 24.0psi @09:36:54.41 SS.reconfigure forSampling @09:36:54.44 SS.seek 25.00ml @09:36:55.42 delay 3 seconds @09:36:58.43 AFV.select :holdingCoil @09:36:58.57 AS.reconfigure ASslow1 @09:36:58.60 AS.seek 0.489ml @09:37:10.78 Vacuum too high -- waiting up to 40s @09:37:11.04 Intake @43.4psia, vacuum from 20.0 to 24.0psi @09:37:11.05 SS.reconfigure forSampling @09:37:11.09 SS.seek 25.00ml @09:37:46.96 Stroke completed -- waiting for pressure to equalize ... @09:37:47.60 Intake.select :close @09:37:47.74 SSV.dial :exhaust @09:37:48.52 Exhaust.select :open @09:37:48.62 SS.reconfigure SSexhale @09:37:48.66 SS.seek 0.00ml @09:37:53.92 delay 5 seconds @09:37:57.79 Exhaust.select :close @09:37:57.89 SSV.dial CBV @09:37:57.91 CBV.dial SSV @09:37:58.69 Allowing 185s for 25.0ml of remaining 925.0ml @09:37:59.26 Intake.select :open @09:37:59.34 AS.reconfigure ASslow2 @09:37:59.38 AS.seek 0.454ml @09:38:01.42 Intake @43.9psia, vacuum from 20.0 to 24.0psi @09:38:01.43 SS.reconfigure forSampling @09:38:01.46 SS.seek 25.00ml @09:38:35.28 delay 1:00 @09:39:00.94 Stroke completed -- waiting for pressure to equalize ... @09:39:01.58 Intake.select :close @09:39:01.72 SSV.dial :exhaust @09:39:02.49 Exhaust.select :open @09:39:02.59 SS.reconfigure SSexhale @09:39:02.63 SS.seek 0.00ml @09:39:11.89 Exhaust.select :close @09:39:11.99 SSV.dial CBV @09:39:12.01 CBV.dial SSV @09:39:12.78 Allowing 185s for 25.0ml of remaining 900.0ml @09:39:12.80 Intake.select :open @09:39:14.94 Intake @42.4psia, vacuum from 20.0 to 24.0psi @09:39:14.95 SS.reconfigure forSampling @09:39:14.98 SS.seek 25.00ml @09:39:35.29 pushing eluate to holding coil @09:39:35.35 AS.seek 0.294ml @09:40:14.78 Stroke completed -- waiting for pressure to equalize ... @09:40:15.42 Intake.select :close @09:40:15.56 SSV.dial :exhaust @09:40:16.33 Exhaust.select :open @09:40:16.43 SS.reconfigure SSexhale @09:40:16.47 SS.seek 0.00ml @09:40:25.26 Exhaust.select :close @09:40:25.36 SSV.dial CBV @09:40:25.38 CBV.dial SSV @09:40:26.17 Allowing 185s for 25.0ml of remaining 875.0ml @09:40:26.18 Intake.select :open @09:40:28.32 Intake @43.7psia, vacuum from 20.0 to 24.0psi @09:40:28.34 SS.reconfigure forSampling @09:40:28.37 SS.seek 25.00ml @09:41:27.51 Stroke completed -- waiting for pressure to equalize ... @09:41:28.15 Intake.select :close @09:41:28.29 SSV.dial :exhaust @09:41:29.06 Exhaust.select :open @09:41:29.16 SS.reconfigure SSexhale @09:41:29.20 SS.seek 0.00ml @09:41:38.01 Exhaust.select :close @09:41:38.11 SSV.dial CBV @09:41:38.13 CBV.dial SSV @09:41:38.90 Allowing 185s for 25.0ml of remaining 850.0ml @09:41:38.91 Intake.select :open @09:41:41.05 Intake @42.1psia, vacuum from 20.0 to 24.0psi @09:41:41.07 SS.reconfigure forSampling @09:41:41.10 SS.seek 25.00ml @09:42:18.71 delay 30 seconds @09:42:40.21 Stroke completed -- waiting for pressure to equalize ... @09:42:40.85 Intake.select :close @09:42:40.99 SSV.dial :exhaust @09:42:41.77 Exhaust.select :open @09:42:41.87 SS.reconfigure SSexhale @09:42:41.91 SS.seek 0.00ml @09:42:48.72 ATV.dial :waste,via => air3 @09:42:50.18 AS.reconfigure ASconfig @09:42:50.21 AS.seek empty @09:42:51.22 Exhaust.select :close @09:42:51.32 SSV.dial CBV @09:42:51.34 CBV.dial SSV @09:42:52.14 Allowing 185s for 25.0ml of remaining 825.0ml @09:42:52.15 Intake.select :open @09:42:54.28 Intake @43.4psia, vacuum from 20.0 to 24.0psi @09:42:54.30 SS.reconfigure forSampling @09:42:54.33 SS.seek 25.00ml @09:43:07.64 Parking Bleach in LysateSource line and SPEcolumn @09:43:07.65 ATV.dial :air3 @09:43:08.32 AS.reconfigure ASslow1 @09:43:08.35 AS.seek 0.029ml @09:43:14.77 ATV.dial :ElutionWater @09:43:15.16 AS.seek 0.059ml @09:43:21.53 ATV.dial :air3 @09:43:22.00 AS.seek 0.069ml @09:43:24.20 delay 2 seconds @09:43:26.21 ATV.dial :bleach @09:43:26.58 AS.seek 0.711ml @09:43:53.54 Stroke completed -- waiting for pressure to equalize ... @09:43:54.18 Intake.select :close @09:43:54.32 SSV.dial :exhaust @09:43:55.09 Exhaust.select :open @09:43:55.19 SS.reconfigure SSexhale @09:43:55.23 SS.seek 0.00ml @09:44:04.50 Exhaust.select :close @09:44:04.60 SSV.dial CBV @09:44:04.62 CBV.dial SSV @09:44:05.40 Allowing 185s for 25.0ml of remaining 800.0ml @09:44:05.41 Intake.select :open @09:44:07.55 Intake @43.6psia, vacuum from 20.0 to 24.0psi @09:44:07.57 SS.reconfigure forSampling @09:44:07.60 SS.seek 25.00ml @09:44:43.48 Vacuum too high -- waiting up to 40s @09:44:43.76 Intake @43.4psia, vacuum from 20.0 to 24.0psi @09:44:43.77 SS.reconfigure forSampling @09:44:43.81 SS.seek 25.00ml @09:45:27.55 Stroke completed -- waiting for pressure to equalize ... @09:45:28.19 Intake.select :close @09:45:28.33 SSV.dial :exhaust @09:45:29.65 Exhaust.select :open @09:45:29.75 SS.reconfigure SSexhale @09:45:29.79 SS.seek 0.00ml @09:45:37.79 delay 3 seconds @09:45:38.74 Exhaust.select :close @09:45:38.84 SSV.dial CBV @09:45:38.86 CBV.dial SSV @09:45:39.64 Allowing 185s for 25.0ml of remaining 775.0ml @09:45:39.65 Intake.select :open @09:45:40.80 ATV.dial :air3 @09:45:41.24 delay 3 seconds @09:45:41.79 Intake @45.0psia, vacuum from 20.0 to 24.0psi @09:45:41.80 SS.reconfigure forSampling @09:45:41.83 SS.seek 25.00ml @09:45:44.29 AS.seek 0.736ml @09:45:49.65 delay 3 seconds @09:45:52.66 ATV.dial :bleach @09:45:53.15 AS.seek 0.721ml @09:45:56.46 delay 3 seconds @09:45:59.47 ATV.dial :AIV @09:46:00.58 AS.reconfigure ASconfig @09:46:00.61 AS.seek 0.015ml @09:46:42.05 ATV.dial :waste @09:46:43.34 AS.seek empty @09:46:44.55 ATV.dial :air3 @09:46:45.12 AS.reconfigure ASslow1 @09:46:45.16 AS.seek 0.009ml @09:46:47.46 delay 2 seconds @09:46:49.47 ATV.dial :bleach @09:46:49.85 AS.seek 0.579ml @09:47:32.14 Stroke completed -- waiting for pressure to equalize ... @09:47:33.37 Intake.select :close @09:47:33.51 SSV.dial :exhaust @09:47:34.28 Exhaust.select :open @09:47:34.38 SS.reconfigure SSexhale @09:47:34.42 SS.seek 0.00ml @09:47:43.64 Exhaust.select :close @09:47:43.74 SSV.dial CBV @09:47:43.76 CBV.dial SSV @09:47:44.55 Allowing 185s for 25.0ml of remaining 750.0ml @09:47:44.56 Intake.select :open @09:47:46.70 Intake @46.1psia, vacuum from 20.0 to 24.0psi @09:47:46.72 SS.reconfigure forSampling @09:47:46.75 SS.seek 25.00ml @09:48:46.36 delay 3 seconds @09:48:49.37 ATV.dial :air3 @09:48:49.70 delay 3 seconds @09:48:52.76 AS.seek 0.604ml @09:48:57.13 Vacuum too high -- waiting up to 40s @09:48:57.61 Intake @39.8psia, vacuum from 20.0 to 24.0psi @09:48:57.62 SS.reconfigure forSampling @09:48:57.66 SS.seek 25.00ml @09:48:58.08 delay 3 seconds @09:49:01.09 ATV.dial :bleach @09:49:01.49 AS.seek 0.589ml @09:49:04.81 delay 3 seconds @09:49:07.82 ATV.dial :ASV @09:49:08.70 ASV.dial :wasteR @09:49:09.21 AS.seek 0.000ml @09:49:37.82 Stroke completed -- waiting for pressure to equalize ... @09:49:39.64 Intake.select :close @09:49:39.78 SSV.dial :exhaust @09:49:40.57 Exhaust.select :open @09:49:40.67 SS.reconfigure SSexhale @09:49:40.71 SS.seek 0.00ml @09:49:49.99 Exhaust.select :close @09:49:50.09 SSV.dial CBV @09:49:50.11 CBV.dial SSV @09:49:50.90 Allowing 185s for 25.0ml of remaining 725.0ml @09:49:50.91 Intake.select :open @09:49:53.05 Intake @44.9psia, vacuum from 20.0 to 24.0psi @09:49:53.07 SS.reconfigure forSampling @09:49:53.10 SS.seek 25.00ml @09:50:41.93 Vacuum too high -- waiting up to 40s @09:50:42.21 Intake @44.4psia, vacuum from 20.0 to 24.0psi @09:50:42.22 SS.reconfigure forSampling @09:50:42.26 SS.seek 25.00ml @09:51:09.64 delay 1:00 @09:51:11.34 Vacuum too high -- waiting up to 40s @09:51:11.60 Intake @44.7psia, vacuum from 20.0 to 24.0psi @09:51:11.62 SS.reconfigure forSampling @09:51:11.65 SS.seek 25.00ml @09:51:15.33 Vacuum too high -- waiting up to 40s @09:51:15.61 SS.seek 25.00ml @09:51:18.37 Vacuum too high -- waiting up to 40s @09:51:18.64 Intake @44.4psia, vacuum from 20.0 to 24.0psi @09:51:18.66 SS.reconfigure forSampling @09:51:18.69 SS.seek 25.00ml @09:51:24.31 Vacuum too high -- waiting up to 40s @09:51:24.78 Intake @42.2psia, vacuum from 20.0 to 24.0psi @09:51:24.80 SS.reconfigure forSampling @09:51:24.83 SS.seek 25.00ml @09:51:45.89 Stroke completed -- waiting for pressure to equalize ... @09:51:48.29 Intake.select :close @09:51:48.43 SSV.dial :exhaust @09:51:49.21 Exhaust.select :open @09:51:49.31 SS.reconfigure SSexhale @09:51:49.35 SS.seek 0.00ml @09:51:58.46 Exhaust.select :close @09:51:58.56 SSV.dial CBV @09:51:58.58 CBV.dial SSV @09:51:59.35 Allowing 185s for 25.0ml of remaining 700.0ml @09:51:59.37 Intake.select :open @09:52:01.51 Intake @45.1psia, vacuum from 20.0 to 24.0psi @09:52:01.52 SS.reconfigure forSampling @09:52:01.55 SS.seek 25.00ml @09:52:03.49 Vacuum too high -- waiting up to 40s @09:52:03.76 Intake @44.8psia, vacuum from 20.0 to 24.0psi @09:52:03.78 SS.reconfigure forSampling @09:52:03.81 SS.seek 25.00ml @09:52:05.15 Vacuum too high -- waiting up to 40s @09:52:05.42 Intake @44.6psia, vacuum from 20.0 to 24.0psi @09:52:05.44 SS.reconfigure forSampling @09:52:05.47 SS.seek 25.00ml @09:52:09.21 Vacuum too high -- waiting up to 40s @09:52:09.69 AFV.select :carrierSolution @09:52:09.74 Intake @44.0psia, vacuum from 20.0 to 24.0psi @09:52:09.75 SS.reconfigure forSampling @09:52:09.78 SS.seek 25.00ml @09:52:09.82 delay 2 seconds @09:52:11.82 AS.reconfigure ASconfig @09:52:11.85 AS.seek 1.000ml @09:52:12.56 Vacuum too high -- waiting up to 40s @09:52:12.84 Intake @45.1psia, vacuum from 20.0 to 24.0psi @09:52:12.85 SS.reconfigure forSampling @09:52:12.88 SS.seek 25.00ml @09:52:14.21 Vacuum too high -- waiting up to 40s @09:52:14.48 Intake @45.1psia, vacuum from 20.0 to 24.0psi @09:52:14.50 SS.reconfigure forSampling @09:52:14.53 SS.seek 25.00ml @09:52:15.96 Vacuum too high -- waiting up to 40s @09:52:16.24 Intake @45.1psia, vacuum from 20.0 to 24.0psi @09:52:16.25 SS.reconfigure forSampling @09:52:16.28 SS.seek 25.00ml @09:52:17.65 Vacuum too high -- waiting up to 40s @09:52:17.92 Intake @45.5psia, vacuum from 20.0 to 24.0psi @09:52:17.94 SS.reconfigure forSampling @09:52:17.97 SS.seek 25.00ml @09:52:19.37 Vacuum too high -- waiting up to 40s @09:52:19.64 Intake @47.0psia, vacuum from 20.0 to 24.0psi @09:52:19.66 SS.reconfigure forSampling @09:52:19.69 SS.seek 25.00ml @09:52:20.62 Vacuum too high -- waiting up to 40s @09:52:20.89 Intake @48.4psia, vacuum from 20.0 to 24.0psi @09:52:20.91 SS.reconfigure forSampling @09:52:20.94 SS.seek 25.00ml @09:52:21.65 Vacuum too high -- waiting up to 40s @09:52:21.92 Intake @45.1psia, vacuum from 20.0 to 24.0psi @09:52:21.94 SS.reconfigure forSampling @09:52:21.97 SS.seek 25.00ml @09:52:23.18 Vacuum too high -- waiting up to 40s @09:52:23.45 Intake @44.9psia, vacuum from 20.0 to 24.0psi @09:52:23.47 SS.reconfigure forSampling @09:52:23.50 SS.seek 25.00ml @09:52:24.84 Vacuum too high -- waiting up to 40s @09:52:25.11 Intake @42.1psia, vacuum from 20.0 to 24.0psi @09:52:25.13 SS.reconfigure forSampling @09:52:25.16 SS.seek 25.00ml @09:52:45.68 Vacuum too high -- waiting up to 40s @09:52:46.49 Intake @44.3psia, vacuum from 20.0 to 24.0psi @09:52:46.51 SS.reconfigure forSampling @09:52:46.54 SS.seek 25.00ml @09:52:48.30 Vacuum too high -- waiting up to 40s @09:52:48.57 Intake @45.6psia, vacuum from 20.0 to 24.0psi @09:52:48.59 SS.reconfigure forSampling @09:52:48.62 SS.seek 25.00ml @09:52:49.71 Vacuum too high -- waiting up to 40s @09:52:49.98 Intake @46.3psia, vacuum from 20.0 to 24.0psi @09:52:50.00 SS.reconfigure forSampling @09:52:50.03 SS.seek 25.00ml @09:52:51.05 Vacuum too high -- waiting up to 40s @09:52:51.33 Intake @44.7psia, vacuum from 20.0 to 24.0psi @09:52:51.34 SS.reconfigure forSampling @09:52:51.38 SS.seek 25.00ml @09:52:52.65 Vacuum too high -- waiting up to 40s @09:52:52.92 Intake @44.1psia, vacuum from 20.0 to 24.0psi @09:52:52.94 SS.reconfigure forSampling @09:52:52.97 SS.seek 25.00ml @09:52:54.24 Vacuum too high -- waiting up to 40s @09:52:54.51 Intake @44.1psia, vacuum from 20.0 to 24.0psi @09:52:54.52 SS.reconfigure forSampling @09:52:55.16 SS.seek 25.00ml @09:52:57.02 Vacuum too high -- waiting up to 40s @09:52:57.30 Intake @44.6psia, vacuum from 20.0 to 24.0psi @09:52:57.32 SS.reconfigure forSampling @09:52:57.35 SS.seek 25.00ml @09:52:58.55 Vacuum too high -- waiting up to 40s @09:52:58.82 Intake @45.1psia, vacuum from 20.0 to 24.0psi @09:52:58.84 SS.reconfigure forSampling @09:52:58.87 SS.seek 25.00ml @09:52:59.90 Vacuum too high -- waiting up to 40s @09:53:00.17 Intake @44.6psia, vacuum from 20.0 to 24.0psi @09:53:00.19 SS.reconfigure forSampling @09:53:00.22 SS.seek 25.00ml @09:53:01.52 Vacuum too high -- waiting up to 40s @09:53:01.80 Intake @44.9psia, vacuum from 20.0 to 24.0psi @09:53:01.81 SS.reconfigure forSampling @09:53:01.84 SS.seek 25.00ml @09:53:02.96 Vacuum too high -- waiting up to 40s @09:53:03.24 SS.seek 25.00ml @09:53:04.37 Vacuum too high -- waiting up to 40s @09:53:04.64 Intake @45.0psia, vacuum from 20.0 to 24.0psi @09:53:04.66 SS.reconfigure forSampling @09:53:04.69 SS.seek 25.00ml @09:53:06.05 Vacuum too high -- waiting up to 40s @09:53:06.33 Intake @45.1psia, vacuum from 20.0 to 24.0psi @09:53:06.35 SS.reconfigure forSampling @09:53:06.38 SS.seek 25.00ml @09:53:07.40 Vacuum too high -- waiting up to 40s @09:53:07.67 Intake @43.3psia, vacuum from 20.0 to 24.0psi @09:53:07.69 SS.reconfigure forSampling @09:53:07.72 SS.seek 25.00ml @09:53:10.11 Vacuum too high -- waiting up to 40s @09:53:10.43 delay 1 second @09:53:10.60 Intake @44.5psia, vacuum from 20.0 to 24.0psi @09:53:10.62 SS.reconfigure forSampling @09:53:10.65 SS.seek 25.00ml @09:53:11.44 AFV.select :holdingCoil @09:53:11.53 delay 1 second @09:53:11.80 Vacuum too high -- waiting up to 40s @09:53:12.08 Intake @44.2psia, vacuum from 20.0 to 24.0psi @09:53:12.09 SS.reconfigure forSampling @09:53:12.12 SS.seek 25.00ml @09:53:12.54 ATV.dial :ASV @09:53:12.55 ASV.dial :wasteR @09:53:12.56 AS.reconfigure ASslow1 @09:53:12.59 AS.seek 0.000ml @09:53:13.55 Vacuum too high -- waiting up to 40s @09:53:14.04 Intake @45.4psia, vacuum from 20.0 to 24.0psi @09:53:14.06 SS.reconfigure forSampling @09:53:14.09 SS.seek 25.00ml @09:53:15.40 Vacuum too high -- waiting up to 40s @09:53:15.68 Intake @45.2psia, vacuum from 20.0 to 24.0psi @09:53:15.69 SS.reconfigure forSampling @09:53:15.72 SS.seek 25.00ml @09:53:16.77 Vacuum too high -- waiting up to 40s @09:53:17.04 Intake @44.8psia, vacuum from 20.0 to 24.0psi @09:53:17.06 SS.reconfigure forSampling @09:53:17.09 SS.seek 25.00ml @09:53:18.27 Vacuum too high -- waiting up to 40s @09:53:18.54 Intake @45.0psia, vacuum from 20.0 to 24.0psi @09:53:18.56 SS.reconfigure forSampling @09:53:18.59 SS.seek 25.00ml @09:53:19.83 Vacuum too high -- waiting up to 40s @09:53:20.10 Intake @45.5psia, vacuum from 20.0 to 24.0psi @09:53:20.12 SS.reconfigure forSampling @09:53:20.15 SS.seek 25.00ml @09:53:21.40 Vacuum too high -- waiting up to 40s @09:53:21.68 Intake @46.3psia, vacuum from 20.0 to 24.0psi @09:53:21.69 SS.reconfigure forSampling @09:53:21.72 SS.seek 25.00ml @09:53:22.77 Vacuum too high -- waiting up to 40s @09:53:23.04 Intake @46.1psia, vacuum from 20.0 to 24.0psi @09:53:23.06 SS.reconfigure forSampling @09:53:23.09 SS.seek 25.00ml @09:53:24.02 Vacuum too high -- waiting up to 40s @09:53:24.29 Intake @44.5psia, vacuum from 20.0 to 24.0psi @09:53:24.31 SS.reconfigure forSampling @09:53:24.34 SS.seek 25.00ml @09:53:25.61 Vacuum too high -- waiting up to 40s @09:53:25.88 Intake @44.8psia, vacuum from 20.0 to 24.0psi @09:53:25.89 SS.reconfigure forSampling @09:53:25.93 SS.seek 25.00ml @09:53:27.11 Vacuum too high -- waiting up to 40s @09:53:27.38 Intake @44.9psia, vacuum from 20.0 to 24.0psi @09:53:27.39 SS.reconfigure forSampling @09:53:27.43 SS.seek 25.00ml @09:53:28.52 Vacuum too high -- waiting up to 40s @09:53:29.40 Intake @44.7psia, vacuum from 20.0 to 24.0psi @09:53:29.42 SS.reconfigure forSampling @09:53:29.45 SS.seek 25.00ml @09:53:31.02 Vacuum too high -- waiting up to 40s @09:53:31.30 Intake @44.3psia, vacuum from 20.0 to 24.0psi @09:53:31.31 SS.reconfigure forSampling @09:53:31.35 SS.seek 25.00ml @09:53:32.89 Vacuum too high -- waiting up to 40s @09:53:33.40 Intake @46.0psia, vacuum from 20.0 to 24.0psi @09:53:33.41 SS.reconfigure forSampling @09:53:33.44 SS.seek 25.00ml @09:53:34.55 Vacuum too high -- waiting up to 40s @09:53:34.82 Intake @46.3psia, vacuum from 20.0 to 24.0psi @09:53:34.84 SS.reconfigure forSampling @09:53:34.87 SS.seek 25.00ml @09:53:35.71 Vacuum too high -- waiting up to 40s @09:53:36.21 Intake @42.7psia, vacuum from 20.0 to 24.0psi @09:53:36.23 SS.reconfigure forSampling @09:53:36.26 SS.seek 25.00ml @09:53:39.67 Vacuum too high -- waiting up to 40s @09:53:40.38 Intake @44.3psia, vacuum from 20.0 to 24.0psi @09:53:40.40 SS.reconfigure forSampling @09:53:40.43 SS.seek 25.00ml @09:53:42.08 Vacuum too high -- waiting up to 40s @09:53:42.57 Intake @44.4psia, vacuum from 20.0 to 24.0psi @09:53:42.59 SS.reconfigure forSampling @09:53:42.62 SS.seek 25.00ml @09:53:43.93 Vacuum too high -- waiting up to 40s @09:53:44.20 Intake @45.1psia, vacuum from 20.0 to 24.0psi @09:53:44.22 SS.reconfigure forSampling @09:53:44.25 SS.seek 25.00ml @09:53:45.21 Vacuum too high -- waiting up to 40s @09:53:45.48 Intake @45.6psia, vacuum from 20.0 to 24.0psi @09:53:45.50 SS.reconfigure forSampling @09:53:45.53 SS.seek 25.00ml @09:53:46.49 Vacuum too high -- waiting up to 40s @09:53:46.76 Intake @46.4psia, vacuum from 20.0 to 24.0psi @09:53:46.78 SS.reconfigure forSampling @09:53:46.81 SS.seek 25.00ml @09:53:47.80 Vacuum too high -- waiting up to 40s @09:53:48.07 Intake @46.2psia, vacuum from 20.0 to 24.0psi @09:53:48.09 SS.reconfigure forSampling @09:53:48.12 SS.seek 25.00ml @09:53:49.24 Vacuum too high -- waiting up to 40s @09:53:49.51 Intake @45.9psia, vacuum from 20.0 to 24.0psi @09:53:49.53 SS.reconfigure forSampling @09:53:49.56 SS.seek 25.00ml @09:53:50.58 Vacuum too high -- waiting up to 40s @09:53:50.85 Intake @45.1psia, vacuum from 20.0 to 24.0psi @09:53:50.87 SS.reconfigure forSampling @09:53:50.90 SS.seek 25.00ml @09:53:52.11 Vacuum too high -- waiting up to 40s @09:53:52.38 Intake @45.2psia, vacuum from 20.0 to 24.0psi @09:53:52.40 SS.reconfigure forSampling @09:53:52.43 SS.seek 25.00ml @09:53:53.55 Vacuum too high -- waiting up to 40s @09:53:53.82 Intake @44.6psia, vacuum from 20.0 to 24.0psi @09:53:53.84 SS.reconfigure forSampling @09:53:53.87 SS.seek 25.00ml @09:53:55.08 Vacuum too high -- waiting up to 40s @09:53:55.35 Intake @42.8psia, vacuum from 20.0 to 24.0psi @09:53:55.37 SS.reconfigure forSampling @09:53:55.40 SS.seek 25.00ml @09:53:57.67 Vacuum too high -- waiting up to 40s @09:53:58.16 Intake @44.2psia, vacuum from 20.0 to 24.0psi @09:53:58.18 SS.reconfigure forSampling @09:53:58.21 SS.seek 25.00ml @09:53:59.92 Vacuum too high -- waiting up to 40s @09:54:00.41 Intake @36.1psia, vacuum from 20.0 to 24.0psi @09:54:00.43 SS.reconfigure forSampling @09:54:00.46 SS.seek 25.00ml @09:54:23.50 Stroke completed -- waiting for pressure to equalize ... @09:54:25.31 Intake.select :close @09:54:25.45 SSV.dial :exhaust @09:54:26.22 Exhaust.select :open @09:54:26.31 SS.reconfigure SSexhale @09:54:26.35 SS.seek 0.00ml @09:54:35.01 Exhaust.select :close @09:54:35.74 SSV.dial CBV @09:54:35.76 CBV.dial SSV @09:54:36.54 Allowing 185s for 25.0ml of remaining 675.0ml @09:54:36.55 Intake.select :open @09:54:38.69 Intake @44.8psia, vacuum from 20.0 to 24.0psi @09:54:38.71 SS.reconfigure forSampling @09:54:38.74 SS.seek 25.00ml @09:54:40.11 Vacuum too high -- waiting up to 40s @09:54:40.39 Intake @44.2psia, vacuum from 20.0 to 24.0psi @09:54:40.40 SS.reconfigure forSampling @09:54:40.43 SS.seek 25.00ml @09:54:41.33 Vacuum too high -- waiting up to 40s @09:54:41.61 Intake @44.5psia, vacuum from 20.0 to 24.0psi @09:54:41.62 SS.reconfigure forSampling @09:54:41.65 SS.seek 25.00ml @09:54:42.58 Vacuum too high -- waiting up to 40s @09:54:42.86 Intake @45.0psia, vacuum from 20.0 to 24.0psi @09:54:42.87 SS.reconfigure forSampling @09:54:42.90 SS.seek 25.00ml @09:54:43.80 Vacuum too high -- waiting up to 40s @09:54:44.09 Intake @45.3psia, vacuum from 20.0 to 24.0psi @09:54:44.10 SS.reconfigure forSampling @09:54:44.13 SS.seek 25.00ml @09:54:44.83 Vacuum too high -- waiting up to 40s @09:54:45.33 Intake @41.2psia, vacuum from 20.0 to 24.0psi @09:54:45.34 SS.reconfigure forSampling @09:54:45.37 SS.seek 25.00ml @09:54:48.77 Vacuum too high -- waiting up to 40s @09:54:49.53 Intake @45.1psia, vacuum from 20.0 to 24.0psi @09:54:49.55 SS.reconfigure forSampling @09:54:49.58 SS.seek 25.00ml @09:54:50.67 Vacuum too high -- waiting up to 40s @09:54:50.94 Intake @41.2psia, vacuum from 20.0 to 24.0psi @09:54:50.96 SS.reconfigure forSampling @09:54:50.99 SS.seek 25.00ml @09:54:53.27 Vacuum too high -- waiting up to 40s @09:54:53.98 Intake @43.5psia, vacuum from 20.0 to 24.0psi @09:54:54.00 SS.reconfigure forSampling @09:54:54.03 SS.seek 25.00ml @09:54:55.23 Vacuum too high -- waiting up to 40s @09:54:55.72 Intake @44.8psia, vacuum from 20.0 to 24.0psi @09:54:55.74 SS.reconfigure forSampling @09:54:55.77 SS.seek 25.00ml @09:54:56.98 Vacuum too high -- waiting up to 40s @09:54:57.25 Intake @47.0psia, vacuum from 20.0 to 24.0psi @09:54:57.27 SS.reconfigure forSampling @09:54:57.30 SS.seek 25.00ml @09:54:57.86 Vacuum too high -- waiting up to 40s @09:54:58.13 Intake @48.0psia, vacuum from 20.0 to 24.0psi @09:54:58.15 SS.reconfigure forSampling @09:54:58.18 SS.seek 25.00ml @09:54:58.67 Vacuum too high -- waiting up to 40s @09:54:59.16 Intake @41.8psia, vacuum from 20.0 to 24.0psi @09:54:59.18 SS.reconfigure forSampling @09:54:59.21 SS.seek 25.00ml @09:55:01.58 Vacuum too high -- waiting up to 40s @09:55:02.07 Intake @44.4psia, vacuum from 20.0 to 24.0psi @09:55:02.09 SS.reconfigure forSampling @09:55:02.12 SS.seek 25.00ml @09:55:03.05 Vacuum too high -- waiting up to 40s @09:55:03.32 Intake @44.0psia, vacuum from 20.0 to 24.0psi @09:55:03.34 SS.reconfigure forSampling @09:55:03.37 SS.seek 25.00ml @09:55:04.27 Vacuum too high -- waiting up to 40s @09:55:04.54 Intake @43.9psia, vacuum from 20.0 to 24.0psi @09:55:04.56 SS.reconfigure forSampling @09:55:04.59 SS.seek 25.00ml @09:55:05.52 Vacuum too high -- waiting up to 40s @09:55:05.79 Intake @44.1psia, vacuum from 20.0 to 24.0psi @09:55:05.81 SS.reconfigure forSampling @09:55:05.84 SS.seek 25.00ml @09:55:06.67 Vacuum too high -- waiting up to 40s @09:55:06.94 Intake @43.6psia, vacuum from 20.0 to 24.0psi @09:55:06.96 SS.reconfigure forSampling @09:55:06.99 SS.seek 25.00ml @09:55:08.05 Vacuum too high -- waiting up to 40s @09:55:08.33 Intake @43.0psia, vacuum from 20.0 to 24.0psi @09:55:08.34 SS.reconfigure forSampling @09:55:08.37 SS.seek 25.00ml @09:55:09.83 Vacuum too high -- waiting up to 40s @09:55:10.95 Intake @45.5psia, vacuum from 20.0 to 24.0psi @09:55:10.96 SS.reconfigure forSampling @09:55:10.99 SS.seek 25.00ml @09:55:12.36 Vacuum too high -- waiting up to 40s @09:55:12.64 Intake @50.7psia, vacuum from 20.0 to 24.0psi @09:55:12.65 SS.reconfigure forSampling @09:55:12.68 SS.seek 25.00ml @09:55:13.01 Vacuum too high -- waiting up to 40s @09:55:13.28 Intake @44.9psia, vacuum from 20.0 to 24.0psi @09:55:13.30 SS.reconfigure forSampling @09:55:13.33 SS.seek 25.00ml @09:55:14.33 Vacuum too high -- waiting up to 40s @09:55:14.60 Intake @44.8psia, vacuum from 20.0 to 24.0psi @09:55:14.62 SS.reconfigure forSampling @09:55:14.65 SS.seek 25.00ml @09:55:15.55 Vacuum too high -- waiting up to 40s @09:55:15.82 Intake @45.3psia, vacuum from 20.0 to 24.0psi @09:55:15.84 SS.reconfigure forSampling @09:55:15.87 SS.seek 25.00ml @09:55:16.64 Vacuum too high -- waiting up to 40s @09:55:16.92 Intake @45.1psia, vacuum from 20.0 to 24.0psi @09:55:16.93 SS.reconfigure forSampling @09:55:16.97 SS.seek 25.00ml @09:55:17.80 Vacuum too high -- waiting up to 40s @09:55:18.07 Intake @45.0psia, vacuum from 20.0 to 24.0psi @09:55:18.09 SS.reconfigure forSampling @09:55:18.12 SS.seek 25.00ml @09:55:18.98 Vacuum too high -- waiting up to 40s @09:55:19.25 Intake @42.3psia, vacuum from 20.0 to 24.0psi @09:55:19.27 SS.reconfigure forSampling @09:55:19.30 SS.seek 25.00ml @09:55:21.39 Vacuum too high -- waiting up to 40s @09:55:21.88 Intake @44.6psia, vacuum from 20.0 to 24.0psi @09:55:21.90 SS.reconfigure forSampling @09:55:21.93 SS.seek 25.00ml @09:55:22.76 Vacuum too high -- waiting up to 40s @09:55:23.03 Intake @45.6psia, vacuum from 20.0 to 24.0psi @09:55:23.05 SS.reconfigure forSampling @09:55:23.08 SS.seek 25.00ml @09:55:23.92 Vacuum too high -- waiting up to 40s @09:55:24.19 Intake @45.9psia, vacuum from 20.0 to 24.0psi @09:55:24.21 SS.reconfigure forSampling @09:55:24.24 SS.seek 25.00ml @09:55:25.01 Vacuum too high -- waiting up to 40s @09:55:25.28 Intake @44.1psia, vacuum from 20.0 to 24.0psi @09:55:25.30 SS.reconfigure forSampling @09:55:25.33 SS.seek 25.00ml @09:55:26.39 Vacuum too high -- waiting up to 40s @09:55:26.89 Intake @44.5psia, vacuum from 20.0 to 24.0psi @09:55:26.91 SS.reconfigure forSampling @09:55:26.94 SS.seek 25.00ml @09:55:27.95 Vacuum too high -- waiting up to 40s @09:55:28.22 Intake @44.7psia, vacuum from 20.0 to 24.0psi @09:55:28.24 SS.reconfigure forSampling @09:55:28.27 SS.seek 25.00ml @09:55:29.17 Vacuum too high -- waiting up to 40s @09:55:29.45 SS.seek 25.00ml @09:55:30.26 Vacuum too high -- waiting up to 40s @09:55:30.53 Intake @44.4psia, vacuum from 20.0 to 24.0psi @09:55:30.55 SS.reconfigure forSampling @09:55:30.58 SS.seek 25.00ml @09:55:31.39 Vacuum too high -- waiting up to 40s @09:55:31.67 SS.seek 25.00ml @09:55:32.58 Vacuum too high -- waiting up to 40s @09:55:33.08 Intake @45.6psia, vacuum from 20.0 to 24.0psi @09:55:33.09 SS.reconfigure forSampling @09:55:33.12 SS.seek 25.00ml @09:55:34.11 Vacuum too high -- waiting up to 40s @09:55:34.38 Intake @46.7psia, vacuum from 20.0 to 24.0psi @09:55:34.40 SS.reconfigure forSampling @09:55:34.43 SS.seek 25.00ml @09:55:35.11 Vacuum too high -- waiting up to 40s @09:55:35.38 Intake @50.4psia, vacuum from 20.0 to 24.0psi @09:55:35.40 SS.reconfigure forSampling @09:55:35.43 SS.seek 25.00ml @09:55:35.83 Vacuum too high -- waiting up to 40s @09:55:36.10 Intake @51.6psia, vacuum from 20.0 to 24.0psi @09:55:36.12 SS.reconfigure forSampling @09:55:36.15 SS.seek 25.00ml @09:55:36.48 Vacuum too high -- waiting up to 40s @09:55:36.75 Intake @44.5psia, vacuum from 20.0 to 24.0psi @09:55:37.38 SS.reconfigure forSampling @09:55:37.41 SS.seek 25.00ml @09:55:38.73 Vacuum too high -- waiting up to 40s @09:55:39.23 Intake @44.7psia, vacuum from 20.0 to 24.0psi @09:55:39.24 SS.reconfigure forSampling @09:55:39.27 SS.seek 25.00ml @09:55:40.26 Vacuum too high -- waiting up to 40s @09:55:40.53 Intake @44.8psia, vacuum from 20.0 to 24.0psi @09:55:40.55 SS.reconfigure forSampling @09:55:40.58 SS.seek 25.00ml @09:55:41.39 Vacuum too high -- waiting up to 40s @09:55:41.66 Intake @44.9psia, vacuum from 20.0 to 24.0psi @09:55:41.68 SS.reconfigure forSampling @09:55:41.71 SS.seek 25.00ml @09:55:42.58 Vacuum too high -- waiting up to 40s @09:55:42.85 Intake @45.1psia, vacuum from 20.0 to 24.0psi @09:55:42.87 SS.reconfigure forSampling @09:55:42.90 SS.seek 25.00ml @09:55:43.76 Vacuum too high -- waiting up to 40s @09:55:44.03 Intake @44.8psia, vacuum from 20.0 to 24.0psi @09:55:44.05 SS.reconfigure forSampling @09:55:44.08 SS.seek 25.00ml @09:55:44.98 Vacuum too high -- waiting up to 40s @09:55:45.25 Intake @45.0psia, vacuum from 20.0 to 24.0psi @09:55:45.27 SS.reconfigure forSampling @09:55:45.30 SS.seek 25.00ml @09:55:46.26 Vacuum too high -- waiting up to 40s @09:55:46.75 Intake @46.2psia, vacuum from 20.0 to 24.0psi @09:55:46.77 SS.reconfigure forSampling @09:55:46.80 SS.seek 25.00ml @09:55:47.64 Vacuum too high -- waiting up to 40s @09:55:47.92 Intake @46.1psia, vacuum from 20.0 to 24.0psi @09:55:47.93 SS.reconfigure forSampling @09:55:47.96 SS.seek 25.00ml @09:55:48.79 Vacuum too high -- waiting up to 40s @09:55:49.28 Intake @43.7psia, vacuum from 20.0 to 24.0psi @09:55:49.30 SS.reconfigure forSampling @09:55:49.33 SS.seek 25.00ml @09:55:51.04 Vacuum too high -- waiting up to 40s @09:55:51.75 Intake @45.4psia, vacuum from 20.0 to 24.0psi @09:55:51.76 SS.reconfigure forSampling @09:55:51.80 SS.seek 25.00ml @09:55:52.89 Vacuum too high -- waiting up to 40s @09:55:53.17 SS.seek 25.00ml @09:55:53.95 Vacuum too high -- waiting up to 40s @09:55:54.22 Intake @45.1psia, vacuum from 20.0 to 24.0psi @09:55:54.24 SS.reconfigure forSampling @09:55:54.27 SS.seek 25.00ml @09:55:55.14 Vacuum too high -- waiting up to 40s @09:55:55.42 Intake @44.9psia, vacuum from 20.0 to 24.0psi @09:55:55.43 SS.reconfigure forSampling @09:55:55.46 SS.seek 25.00ml @09:55:56.36 Vacuum too high -- waiting up to 40s @09:55:56.63 Intake @45.1psia, vacuum from 20.0 to 24.0psi @09:55:56.65 SS.reconfigure forSampling @09:55:56.68 SS.seek 25.00ml @09:55:57.54 Vacuum too high -- waiting up to 40s @09:55:58.03 Intake @45.0psia, vacuum from 20.0 to 24.0psi @09:55:58.05 SS.reconfigure forSampling @09:55:58.08 SS.seek 25.00ml @09:55:59.07 Vacuum too high -- waiting up to 40s @09:55:59.34 Intake @45.0psia, vacuum from 20.0 to 24.0psi @09:55:59.36 SS.reconfigure forSampling @09:55:59.39 SS.seek 25.00ml @09:56:00.20 Vacuum too high -- waiting up to 40s @09:56:00.69 Intake @45.9psia, vacuum from 20.0 to 24.0psi @09:56:00.71 SS.reconfigure forSampling @09:56:00.74 SS.seek 25.00ml @09:56:01.70 Vacuum too high -- waiting up to 40s @09:56:01.97 Intake @45.6psia, vacuum from 20.0 to 24.0psi @09:56:01.99 SS.reconfigure forSampling @09:56:02.02 SS.seek 25.00ml @09:56:02.82 Vacuum too high -- waiting up to 40s @09:56:03.09 Intake @45.2psia, vacuum from 20.0 to 24.0psi @09:56:03.11 SS.reconfigure forSampling @09:56:03.14 SS.seek 25.00ml @09:56:03.95 Vacuum too high -- waiting up to 40s @09:56:04.22 Intake @44.7psia, vacuum from 20.0 to 24.0psi @09:56:04.24 SS.reconfigure forSampling @09:56:04.27 SS.seek 25.00ml @09:56:05.07 Vacuum too high -- waiting up to 40s @09:56:05.93 Intake @45.5psia, vacuum from 20.0 to 24.0psi @09:56:05.95 SS.reconfigure forSampling @09:56:05.98 SS.seek 25.00ml @09:56:07.20 Vacuum too high -- waiting up to 40s @09:56:07.70 Intake @46.7psia, vacuum from 20.0 to 24.0psi @09:56:07.71 SS.reconfigure forSampling @09:56:07.74 SS.seek 25.00ml @09:56:08.70 Vacuum too high -- waiting up to 40s @09:56:08.98 Intake @46.5psia, vacuum from 20.0 to 24.0psi @09:56:08.99 SS.reconfigure forSampling @09:56:09.02 SS.seek 25.00ml @09:56:09.82 Vacuum too high -- waiting up to 40s @09:56:10.09 Intake @46.0psia, vacuum from 20.0 to 24.0psi @09:56:10.11 SS.reconfigure forSampling @09:56:10.14 SS.seek 25.00ml @09:56:10.86 Vacuum too high -- waiting up to 40s @09:56:11.13 Intake @46.0psia, vacuum from 20.0 to 24.0psi @09:56:11.15 SS.reconfigure forSampling @09:56:11.18 SS.seek 25.00ml @09:56:11.92 Vacuum too high -- waiting up to 40s @09:56:12.20 Intake @44.2psia, vacuum from 20.0 to 24.0psi @09:56:12.22 SS.reconfigure forSampling @09:56:12.25 SS.seek 25.00ml @09:56:13.32 Vacuum too high -- waiting up to 40s @09:56:13.81 Intake @44.2psia, vacuum from 20.0 to 24.0psi @09:56:13.83 SS.reconfigure forSampling @09:56:13.86 SS.seek 25.00ml @09:56:15.04 Vacuum too high -- waiting up to 40s @09:56:15.53 Intake @43.9psia, vacuum from 20.0 to 24.0psi @09:56:15.55 SS.reconfigure forSampling @09:56:15.58 SS.seek 25.00ml @09:56:16.86 Vacuum too high -- waiting up to 40s @09:56:17.36 Intake @45.1psia, vacuum from 20.0 to 24.0psi @09:56:17.38 SS.reconfigure forSampling @09:56:17.41 SS.seek 25.00ml @09:56:18.29 Vacuum too high -- waiting up to 40s @09:56:18.78 Intake @42.3psia, vacuum from 20.0 to 24.0psi @09:56:18.80 SS.reconfigure forSampling @09:56:18.83 SS.seek 25.00ml @09:56:20.92 Vacuum too high -- waiting up to 40s @09:56:21.63 Intake @44.5psia, vacuum from 20.0 to 24.0psi @09:56:21.65 SS.reconfigure forSampling @09:56:21.68 SS.seek 25.00ml @09:56:22.92 Vacuum too high -- waiting up to 40s @09:56:23.41 Intake @44.5psia, vacuum from 20.0 to 24.0psi @09:56:23.43 SS.reconfigure forSampling @09:56:23.46 SS.seek 25.00ml @09:56:24.48 Vacuum too high -- waiting up to 40s @09:56:24.97 Intake @44.2psia, vacuum from 20.0 to 24.0psi @09:56:24.99 SS.reconfigure forSampling @09:56:25.02 SS.seek 25.00ml @09:56:26.39 Vacuum too high -- waiting up to 40s @09:56:26.88 Intake @44.2psia, vacuum from 20.0 to 24.0psi @09:56:26.90 SS.reconfigure forSampling @09:56:26.93 SS.seek 25.00ml @09:56:28.17 Vacuum too high -- waiting up to 40s @09:56:28.66 Intake @44.8psia, vacuum from 20.0 to 24.0psi @09:56:28.68 SS.reconfigure forSampling @09:56:28.71 SS.seek 25.00ml @09:56:29.70 Vacuum too high -- waiting up to 40s @09:56:30.19 Intake @45.3psia, vacuum from 20.0 to 24.0psi @09:56:30.20 SS.reconfigure forSampling @09:56:30.24 SS.seek 25.00ml @09:56:31.17 Vacuum too high -- waiting up to 40s @09:56:31.44 Intake @46.1psia, vacuum from 20.0 to 24.0psi @09:56:31.46 SS.reconfigure forSampling @09:56:31.49 SS.seek 25.00ml @09:56:32.23 Vacuum too high -- waiting up to 40s @09:56:32.73 SS.seek 25.00ml @09:56:33.64 Vacuum too high -- waiting up to 40s @09:56:33.92 Intake @47.4psia, vacuum from 20.0 to 24.0psi @09:56:33.93 SS.reconfigure forSampling @09:56:33.96 SS.seek 25.00ml @09:56:35.64 Vacuum too high -- waiting up to 40s @09:56:35.92 Intake @45.0psia, vacuum from 20.0 to 24.0psi @09:56:35.93 SS.reconfigure forSampling @09:56:35.96 SS.seek 25.00ml @09:56:36.85 delay 20 seconds @09:56:36.92 Vacuum too high -- waiting up to 40s @09:56:37.41 Intake @44.9psia, vacuum from 20.0 to 24.0psi @09:56:37.43 SS.reconfigure forSampling @09:56:37.46 SS.seek 25.00ml @09:56:38.48 Vacuum too high -- waiting up to 40s @09:56:38.97 Intake @45.1psia, vacuum from 20.0 to 24.0psi @09:56:38.99 SS.reconfigure forSampling @09:56:39.02 SS.seek 25.00ml @09:56:40.07 Vacuum too high -- waiting up to 40s @09:56:40.57 Intake @44.8psia, vacuum from 20.0 to 24.0psi @09:56:40.58 SS.reconfigure forSampling @09:56:40.61 SS.seek 25.00ml @09:56:41.82 Vacuum too high -- waiting up to 40s @09:56:42.31 Intake @44.3psia, vacuum from 20.0 to 24.0psi @09:56:42.33 SS.reconfigure forSampling @09:56:42.36 SS.seek 25.00ml @09:56:43.51 Vacuum too high -- waiting up to 40s @09:56:44.00 Intake @44.7psia, vacuum from 20.0 to 24.0psi @09:56:44.02 SS.reconfigure forSampling @09:56:44.05 SS.seek 25.00ml @09:56:45.10 Vacuum too high -- waiting up to 40s @09:56:45.38 Intake @45.4psia, vacuum from 20.0 to 24.0psi @09:56:45.39 SS.reconfigure forSampling @09:56:45.42 SS.seek 25.00ml @09:56:46.13 Vacuum too high -- waiting up to 40s @09:56:46.40 Intake @44.3psia, vacuum from 20.0 to 24.0psi @09:56:46.42 SS.reconfigure forSampling @09:56:46.45 SS.seek 25.00ml @09:56:47.57 Vacuum too high -- waiting up to 40s @09:56:48.06 Intake @43.5psia, vacuum from 20.0 to 24.0psi @09:56:48.08 SS.reconfigure forSampling @09:56:48.11 SS.seek 25.00ml @09:56:49.35 Vacuum too high -- waiting up to 40s @09:56:49.84 Intake @42.7psia, vacuum from 20.0 to 24.0psi @09:56:49.86 SS.reconfigure forSampling @09:56:49.89 SS.seek 25.00ml @09:56:51.29 Vacuum too high -- waiting up to 40s @09:56:52.00 Intake @42.4psia, vacuum from 20.0 to 24.0psi @09:56:52.02 SS.reconfigure forSampling @09:56:52.05 SS.seek 25.00ml @09:56:53.63 Vacuum too high -- waiting up to 40s @09:56:54.12 Intake @42.8psia, vacuum from 20.0 to 24.0psi @09:56:54.14 SS.reconfigure forSampling @09:56:54.17 SS.seek 25.00ml @09:56:55.51 Vacuum too high -- waiting up to 40s @09:56:56.24 Intake @42.7psia, vacuum from 20.0 to 24.0psi @09:56:56.25 SS.reconfigure forSampling @09:56:56.28 SS.seek 25.00ml @09:56:56.86 ATV.dial :air3 @09:56:57.96 Vacuum too high -- waiting up to 40s @09:56:58.03 AS.seek 0.590ml @09:56:58.66 Intake @45.0psia, vacuum from 20.0 to 24.0psi @09:56:58.68 SS.reconfigure forSampling @09:56:58.71 SS.seek 25.00ml @09:56:59.70 Vacuum too high -- waiting up to 40s @09:56:59.97 Intake @44.7psia, vacuum from 20.0 to 24.0psi @09:56:59.99 SS.reconfigure forSampling @09:57:00.02 SS.seek 25.00ml @09:57:00.85 Vacuum too high -- waiting up to 40s @09:57:01.35 Intake @42.7psia, vacuum from 20.0 to 24.0psi @09:57:01.37 SS.reconfigure forSampling @09:57:01.40 SS.seek 25.00ml @09:57:02.82 Vacuum too high -- waiting up to 40s @09:57:03.52 Intake @43.3psia, vacuum from 20.0 to 24.0psi @09:57:03.54 SS.reconfigure forSampling @09:57:03.57 SS.seek 25.00ml @09:57:06.10 Vacuum too high -- waiting up to 40s @09:57:06.81 Intake @44.0psia, vacuum from 20.0 to 24.0psi @09:57:06.83 SS.reconfigure forSampling @09:57:06.86 SS.seek 25.00ml @09:57:08.32 Vacuum too high -- waiting up to 40s @09:57:08.82 Intake @42.5psia, vacuum from 20.0 to 24.0psi @09:57:08.83 SS.reconfigure forSampling @09:57:08.86 SS.seek 25.00ml @09:57:10.17 Vacuum too high -- waiting up to 40s @09:57:10.67 Intake @43.2psia, vacuum from 20.0 to 24.0psi @09:57:10.68 SS.reconfigure forSampling @09:57:10.71 SS.seek 25.00ml @09:57:12.26 Vacuum too high -- waiting up to 40s @09:57:12.97 Intake @45.1psia, vacuum from 20.0 to 24.0psi @09:57:12.99 SS.reconfigure forSampling @09:57:13.02 SS.seek 25.00ml @09:57:14.13 Vacuum too high -- waiting up to 40s @09:57:14.84 Intake @43.4psia, vacuum from 20.0 to 24.0psi @09:57:14.86 SS.reconfigure forSampling @09:57:14.89 SS.seek 25.00ml @09:57:16.23 Vacuum too high -- waiting up to 40s @09:57:16.72 Intake @42.1psia, vacuum from 20.0 to 24.0psi @09:57:16.74 SS.reconfigure forSampling @09:57:16.77 SS.seek 25.00ml @09:57:18.41 Vacuum too high -- waiting up to 40s @09:57:19.12 Intake @43.9psia, vacuum from 20.0 to 24.0psi @09:57:19.14 SS.reconfigure forSampling @09:57:19.17 SS.seek 25.00ml @09:57:20.54 Vacuum too high -- waiting up to 40s @09:57:21.03 Intake @43.5psia, vacuum from 20.0 to 24.0psi @09:57:21.05 SS.reconfigure forSampling @09:57:21.08 SS.seek 25.00ml @09:57:22.29 Vacuum too high -- waiting up to 40s @09:57:22.78 Intake @43.4psia, vacuum from 20.0 to 24.0psi @09:57:22.80 SS.reconfigure forSampling @09:57:22.83 SS.seek 25.00ml @09:57:24.29 Vacuum too high -- waiting up to 40s @09:57:25.00 Intake @43.9psia, vacuum from 20.0 to 24.0psi @09:57:25.02 SS.reconfigure forSampling @09:57:25.05 SS.seek 25.00ml @09:57:26.57 Vacuum too high -- waiting up to 40s @09:57:27.28 Intake @45.3psia, vacuum from 20.0 to 24.0psi @09:57:27.30 SS.reconfigure forSampling @09:57:27.33 SS.seek 25.00ml @09:57:28.45 Vacuum too high -- waiting up to 40s @09:57:28.94 Intake @45.7psia, vacuum from 20.0 to 24.0psi @09:57:28.96 SS.reconfigure forSampling @09:57:28.99 SS.seek 25.00ml @09:57:29.79 Vacuum too high -- waiting up to 40s @09:57:30.29 Intake @41.7psia, vacuum from 20.0 to 24.0psi @09:57:30.30 SS.reconfigure forSampling @09:57:30.33 SS.seek 25.00ml @09:57:32.16 Vacuum too high -- waiting up to 40s @09:57:32.87 Intake @44.6psia, vacuum from 20.0 to 24.0psi @09:57:32.88 SS.reconfigure forSampling @09:57:32.92 SS.seek 25.00ml @09:57:34.16 Vacuum too high -- waiting up to 40s @09:57:34.65 Intake @44.7psia, vacuum from 20.0 to 24.0psi @09:57:34.66 SS.reconfigure forSampling @09:57:34.70 SS.seek 25.00ml @09:57:35.73 Vacuum too high -- waiting up to 40s @09:57:36.23 Intake @44.5psia, vacuum from 20.0 to 24.0psi @09:57:36.24 SS.reconfigure forSampling @09:57:36.27 SS.seek 25.00ml @09:57:37.54 Vacuum too high -- waiting up to 40s @09:57:38.47 Intake @44.6psia, vacuum from 20.0 to 24.0psi @09:57:38.49 SS.reconfigure forSampling @09:57:38.52 SS.seek 25.00ml @09:57:40.01 Vacuum too high -- waiting up to 40s @09:57:40.50 Intake @44.7psia, vacuum from 20.0 to 24.0psi @09:57:40.52 SS.reconfigure forSampling @09:57:40.55 SS.seek 25.00ml @09:57:41.55 SS.reconfigure SSconfig @09:57:41.61 SS.hold @09:57:41.70 Intake.select :close @09:57:41.85 ShallowSampler::Clogged in WCR -- progress too slow after 348.9ml @09:57:41.86 SSV.dial :exhaust @09:57:42.64 Exhaust.select :open @09:57:42.74 SS.reconfigure SSexhale @09:57:42.78 SS.seek 0.00ml @09:57:51.24 Exhaust.select :close @09:57:51.35 Sampled 348.9ml @09:57:51.36 collectSample: Complete @09:57:51.37 Drying puck before fixing for 04:00.00 @09:57:51.38 CBV.dial :waste2b @09:57:52.15 delay 1:00 @09:57:54.34 Sleepy.power :sampler,:OFF @09:58:52.15 CSV.dial :air @09:58:52.61 CS.reconfigure CSfast @09:58:52.64 CS.seek 10.000ml @09:58:58.65 delay 3 seconds @09:59:01.66 ATV.dial :ASV @09:59:02.83 ASV.dial :wasteR @09:59:02.89 AS.seek 0.090ml @09:59:16.38 CBV.dial between CSV and waste2b @09:59:16.71 Pumping wcr puck from tube 2 up to 55psi... @09:59:16.76 CBV.dial SSV @09:59:18.18 CBV.dial between CSV and waste2b @09:59:19.11 CSV.dial between air and CTV @09:59:19.49 CS.seek 6.594ml @09:59:28.13 CTV.dial CSV,avoiding => air @09:59:28.15 CSV.dial CTV @09:59:28.90 CS.reconfigure CSpressurize @09:59:28.93 CS.seek 0.000ml @09:59:42.46 CBV.dial :waste2b @09:59:42.77 CS.reconfigure CSconfig @09:59:42.80 CS.seek empty @09:59:47.16 delay 1:00 @10:00:45.13 delay 30 seconds @10:00:47.18 CTV.dial :air,avoiding => intake @10:00:48.45 Fix #1 of 2 (with rnal) for 02:00.00 @10:00:48.46 CS Pulling 2ml of rnal CSV.dial CSR @10:00:48.88 CSR.select :rnal @10:00:48.98 CS.seek 2.000ml @10:00:54.94 delay 5 seconds @10:00:59.95 CSR.select :air @10:01:00.04 CS.reconfigure CSfast @10:01:00.08 CS.seek 10.000ml @10:01:15.14 ATV.dial :extSrc @10:01:15.66 delay 3 seconds @10:01:18.67 ATV.dial :ElutionWater @10:01:19.19 CBV.dial between CSV and waste2b @10:01:19.51 Pumping wcr puck from tube 2 up to 55psi... @10:01:19.57 CBV.dial SSV @10:01:19.65 AS.seek 0.120ml @10:01:21.00 CBV.dial between CSV and waste2b @10:01:21.94 CSV.dial between air and CTV @10:01:22.59 CS.seek 6.538ml @10:01:26.07 ATV.dial :air3 @10:01:26.46 AS.seek 0.130ml @10:01:28.82 delay 2 seconds @10:01:30.83 ATV.dial :bleach @10:01:31.21 AS.seek 0.230ml @10:01:31.26 CTV.dial CSV,avoiding => air @10:01:31.28 CSV.dial CTV @10:01:32.59 CS.reconfigure CSpressurize @10:01:32.62 CS.seek 0.000ml @10:01:48.90 delay 2:00 @10:01:51.89 delay 3 seconds @10:01:54.90 ATV.dial :air3 @10:01:55.24 delay 3 seconds @10:01:58.30 AS.seek 0.255ml @10:02:03.66 delay 3 seconds @10:02:06.67 ATV.dial :bleach @10:02:07.07 AS.seek 0.240ml @10:02:10.39 delay 3 seconds @10:02:13.40 ATV.dial :ASV @10:02:14.38 ASV.dial :SPEdelivery @10:02:14.85 AFV.select :carrierSolution @10:02:14.95 delay 2 seconds @10:02:16.96 AS.reconfigure ASconfig @10:02:16.99 AS.seek 0.500ml @10:02:32.42 delay 1 second @10:02:33.43 AFV.select :holdingCoil @10:02:33.57 AS.reconfigure ASslow1 @10:02:33.60 AS.seek 0.170ml @10:03:41.21 SPE.hold 2:46:40,95.0C @10:03:41.24 delay 3:00 @10:03:48.91 CBV.dial :waste2b @10:03:49.78 CS.reconfigure CSconfig @10:03:49.82 CS.seek empty @10:03:51.31 delay 1:00 @10:04:51.32 CTV.dial :air,avoiding => intake @10:04:52.65 Fix #2 of 2 (with rnal) for 02:00.00 @10:04:52.66 CS Pulling 2ml of rnal CSV.dial CSR @10:04:53.10 CSR.select :rnal @10:04:53.20 CS.seek 2.000ml @10:04:59.06 delay 5 seconds @10:05:04.07 CSR.select :air @10:05:04.16 CS.reconfigure CSfast @10:05:04.20 CS.seek 10.000ml @10:05:23.96 CBV.dial between CSV and waste2b @10:05:24.30 Pumping wcr puck from tube 2 up to 55psi... @10:05:24.35 CBV.dial SSV @10:05:25.80 CBV.dial between CSV and waste2b @10:05:26.76 CSV.dial between air and CTV @10:05:27.44 CS.seek 6.471ml @10:05:36.60 CTV.dial CSV,avoiding => air @10:05:36.62 CSV.dial CTV @10:05:37.97 CS.reconfigure CSpressurize @10:05:38.00 CS.seek 0.000ml @10:05:53.86 delay 2:00 @10:06:41.24 AFV.select :carrierSolution @10:06:41.33 delay 2 seconds @10:06:43.34 AS.reconfigure ASconfig @10:06:43.37 AS.seek 1.000ml @10:07:32.04 delay 1 second @10:07:33.05 AFV.select :holdingCoil @10:07:33.14 delay 1 second @10:07:34.15 ASV.dial :wasteD @10:07:35.44 AS.seek 0.000ml @10:07:53.87 CBV.dial :waste2b @10:07:54.22 CS.reconfigure CSconfig @10:07:54.25 CS.seek empty @10:07:55.96 delay 1:00 @10:08:34.04 Priming Eluate @10:08:34.05 AFV.select :carrierSolution @10:08:34.14 delay 2 seconds @10:08:36.15 AS.seek 0.500ml @10:08:55.97 CTV.dial :air,avoiding => intake @10:08:57.30 Storing wcr puck in Collection Clamp to tube 1 @10:08:57.44 SC.reconfigure SCtight @10:08:57.48 SC.seek 1 @10:08:59.65 SC.reconfigure SCloose @10:08:59.83 Elbow.reconfigure TightElbow @10:08:59.86 Elbow.seek 1 @10:09:01.51 Forearm.reconfigure TightForearm @10:09:01.54 Forearm.seek 1 + 800 counts @10:09:02.55 SE.reconfigure SEpick @10:09:02.60 SE.seek beamBreak @10:09:04.03 SE.configure SEconfig @10:09:04.15 Elbow.reconfigure LimpElbow @10:09:04.19 Forearm.reconfigure LooseForearm @10:09:04.23 Forearm.seek 1 @10:09:04.47 Elbow.hold @10:09:04.53 Elbow.reconfigure TightElbow @10:09:04.60 SE.seek 16.05 Pucks below Top Plate @10:09:04.97 Forearm.coast @10:09:05.01 Forearm.seek 1 + 330 counts @10:09:05.26 Forearm.reconfigure TightForearm @10:09:05.29 Forearm.seek 1 + 800 counts @10:09:05.58 delay 1 second @10:09:05.61 SE.seek 17.05 Pucks below Top Plate @10:09:06.32 Forearm.reconfigure LooseForearm @10:09:06.35 Forearm.seek retract @10:09:06.59 AFV.select :holdingCoil @10:09:06.68 ATV.dial ASV @10:09:06.69 ASV.dial :SPErecovery @10:09:07.19 Swish #1 of 2 @10:09:07.25 AS.reconfigure ASslow0 @10:09:07.30 AS.seek 0.600ml @10:09:07.32 Elbow.seek CC @10:09:08.99 Elbow.reconfigure LooseElbow @10:09:09.07 Forearm.seek CC @10:09:10.04 CC.seek sealed @10:09:10.98 Hand.grip @10:09:11.70 CC.seek open @10:09:15.67 Forearm.seek retract @10:09:16.62 Elbow.reconfigure TightElbow @10:09:16.65 Elbow.seek 1 @10:09:17.84 AS.seek 0.500ml @10:09:18.27 Elbow.reconfigure LimpElbow @10:09:18.32 Forearm.seek 1 @10:09:19.31 Elbow.hold @10:09:19.36 Elbow.reconfigure TightElbow @10:09:19.40 Hand.release @10:09:20.29 Forearm.coast @10:09:20.32 Forearm.seek 1 + 327 counts @10:09:20.57 Forearm.reconfigure TightForearm @10:09:20.61 Forearm.seek 1 + 800 counts @10:09:20.94 SE.seek down @10:09:22.05 Forearm.reconfigure LooseForearm @10:09:22.09 SE.coast @10:09:22.10 Forearm.seek retract @10:09:23.05 SC.coast @10:09:23.09 Starting finalPurge @10:09:23.10 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @10:09:23.25 Elbow.seek FlushPuck::Garage @10:09:25.00 Elbow.reconfigure LooseElbow @10:09:25.08 Forearm.seek garage @10:09:26.06 Hand.grip @10:09:26.91 Forearm.seek retract @10:09:27.92 Elbow.reconfigure TightElbow @10:09:27.95 Elbow.seek CC @10:09:28.35 Swish #2 of 2 @10:09:28.41 AS.seek 0.600ml @10:09:30.72
Elbow.reconfigure LooseElbow @10:09:30.80 Forearm.reconfigure TightForearm @10:09:30.83 Forearm.seek CC @10:09:31.97 CC.seek closed @10:09:35.70 Collection Clamp onSpring/offSpring for 5/22 samples, on/off torque 2.01 @10:09:35.75 Hand.release @10:09:36.49 Forearm.reconfigure LooseForearm @10:09:36.52 Forearm.seek retract @10:09:37.44 Flushing the Collection Valve::Manifold @10:09:37.54 CS Pulling 5ml of flush @10:09:37.55 CSV.dial CSR @10:09:37.97 CSR.select :flush @10:09:38.07 CS.seek 5.000ml @10:09:38.96 AS.seek 0.500ml @10:09:49.44 delay 3 seconds @10:09:51.58
delay 5 seconds @10:09:52.50 AS.reconfigure ASslow1 @10:09:52.53 AS.seek 0.676ml @10:09:56.59
CSR.select :air @10:09:56.73 CS.reconfigure CSfast @10:09:56.76 CS.seek 7.500ml @10:10:03.36 CSV.dial :waste @10:10:04.63 CS.reconfigure CSconfig @10:10:04.66 CS.seek 0.000ml @10:10:24.42 Flushing lower manifold's waste1 port @10:10:24.51 CSV.dial :flush @10:10:25.16 CS.reconfigure CSfast @10:10:25.19 CS.seek 5.000ml @10:10:28.72 delay 3 seconds @10:10:31.73 Eluate primed @10:10:31.74 ATV.dial :waste2 @10:10:32.28 AS.reconfigure ASnorm @10:10:32.31 AS.seek 0.000ml @10:10:37.54
delay 5 seconds @10:10:43.21 CSV.dial :air @10:10:43.78 CS.seek 7.000ml @10:10:49.29 CSV.dial CTV @10:10:49.32
CTV.dial CSV @10:10:50.64 CBV.dial :waste1 @10:10:51.41 CS.seek 0.000ml @10:11:07.19 SPE.coast @10:11:07.27 ASV.dial :SPErecovery @10:11:07.28 Setting up PCR for HF183em -- writing /var/log/esp/HF183em14feb1810h323ml.pcr @10:11:07.29 Building PCR Slug from ASV with MM2 mix, PP4 primer @10:11:07.30 Priming with carrier @10:11:07.31 ATV.dial ARV @10:11:08.02 ARV.dial :carrier @10:11:08.10
Flushing lower manifold's waste2b port @10:11:08.19 CSV.dial :flush @10:11:08.30 AS.seek 0.500ml @10:11:08.99
CS.seek 5.000ml @10:11:21.04 delay 5 seconds @10:11:26.05 CSV.dial :air @10:11:26.61 CS.seek 6.999ml @10:11:32.01 CSV.dial CTV @10:11:32.03
CTV.dial CSV @10:11:32.47 CBV.dial :waste2b @10:11:33.21 CS.seek 0.000ml @10:11:34.17 AFV.select :carrierSolution @10:11:34.27 delay 2 seconds @10:11:36.28 AS.reconfigure ASconfig @10:11:36.32 AS.seek 1.000ml @10:11:49.79
Flushing upper manifold @10:11:49.99 CSV.dial :flush @10:11:50.78 CS.seek 5.000ml @10:12:02.79 delay 5 seconds @10:12:05.78 delay 1 second @10:12:06.79 AFV.select :holdingCoil @10:12:06.88 delay 1 second @10:12:07.80
CSV.dial :air @10:12:07.89 ATV.dial :waste @10:12:08.35
CS.seek 7.000ml @10:12:09.17 AS.reconfigure ASnorm @10:12:09.20 AS.seek empty @10:12:13.73 CSV.dial CBV @10:12:13.75
CBV.dial CSV @10:12:14.87 CTV.dial :air @10:12:16.16 CS.seek 0.000ml @10:12:32.85 delay 7 seconds @10:12:39.86 CS.seek 3.000ml @10:12:47.47 CSV.dial :waste @10:12:47.98 CS.seek 0.000ml @10:12:55.72 CSV.dial :flush @10:12:56.16 CS.seek 3.000ml @10:13:00.67 AFV.select :carrierSolution @10:13:00.78 delay 2 seconds @10:13:02.79 AS.reconfigure ASconfig @10:13:02.82 AS.seek 0.500ml @10:13:03.82
delay 5 seconds @10:13:08.83 CSV.dial :air @10:13:09.30 CS.seek 5.000ml @10:13:14.69 CSV.dial :air @10:13:14.71 CS.seek 0.000ml @10:13:26.82 delay 7 seconds @10:13:32.31 delay 1 second @10:13:33.32 AFV.select :holdingCoil @10:13:33.41 Building Slug @10:13:33.42 ATV.dial :ARV @10:13:33.83
CS.seek 3.000ml @10:13:34.69 delay 2 seconds @10:13:36.69 ARV.dial :air1 @10:13:37.04 delay 2 seconds @10:13:39.09 AS.reconfigure ASslow2 @10:13:39.12 AS.seek 0.506ml @10:13:41.47
CSV.dial :waste @10:13:42.26 CS.seek 0.000ml @10:13:45.57 delay 2 seconds @10:13:47.58 ARV.dial :MM2 @10:13:48.89 delay 2 seconds @10:13:50.07
Disinfecting external Intake with mfbkill @10:13:50.16 CSV.dial CSR @10:13:50.90 Adding MM2 @10:13:50.96 AS.seek 0.524ml @10:13:51.89
CSR.select :mfbkill @10:13:51.99 CS.seek 8.000ml @10:14:09.59 delay 25 seconds @10:14:10.85
delay 5 seconds @10:14:15.86 CSR.select :air @10:14:15.95 CS.seek 10.000ml @10:14:21.31 CSV.dial CBV @10:14:21.33
CBV.dial CSV @10:14:22.33 CTV.dial :intake @10:14:23.03 Intake.select :open @10:14:23.12 CS.seek 2.000ml @10:14:34.60 ARV.dial :PP4 @10:14:34.93 Adding PP4 @10:14:34.95 delay 2 seconds @10:14:37.00 AS.seek 0.530ml @10:14:42.19
Intake.select :close @10:14:42.28 CTV.dial :air @10:14:43.03 CS.reconfigure CSconfig @10:14:43.66 delay 10 seconds @10:14:43.68
CS.seek 0.000ml @10:14:49.56 Flushing lower manifold's waste1 port @10:14:49.57 CS Pulling 4ml of mfbkill @10:14:49.58 CSV.dial CSR @10:14:50.63 CSR.select :mfbkill @10:14:50.73 CS.seek 4.000ml @10:14:53.67 ARV.dial :air3 @10:14:54.07 adding ARV air @10:14:54.08 delay 2 seconds @10:14:56.14 AS.seek 0.536ml @10:15:01.66
delay 5 seconds @10:15:02.67 delay 5 seconds @10:15:06.67
CSR.select :air @10:15:06.76 CS.reconfigure CSfast @10:15:06.80 CS.seek 10.000ml @10:15:07.68 ARV.dial :carrier @10:15:08.72 delay 7 seconds @10:15:15.77 AS.seek 0.594ml @10:15:21.23 CSV.dial CTV @10:15:21.25
CTV.dial CSV @10:15:22.58 CBV.dial :waste1 @10:15:23.04 CS.seek 0.000ml @10:15:46.47 Flushing lower manifold's waste2b port @10:15:46.48 CS Pulling 4ml of mfbkill @10:15:46.49 CSV.dial CSR @10:15:47.00 CSR.select :mfbkill @10:15:47.10 CS.reconfigure CSconfig @10:15:47.14 CS.seek 4.000ml @10:15:58.06 delay 5 seconds @10:16:03.07 CSR.select :air @10:16:03.16 CS.reconfigure CSfast @10:16:03.20 CS.seek 10.000ml @10:16:14.99 delay 5 seconds @10:16:17.54 CSV.dial CTV @10:16:17.56
CTV.dial CSV @10:16:18.07 CBV.dial :waste2b @10:16:18.82 CS.seek 0.000ml @10:16:20.00 ATV.dial :ASV @10:16:20.42 delay 2 seconds @10:16:22.47 AS.seek 0.600ml @10:16:28.83 delay 4 seconds @10:16:32.84 ATV.dial ARV @10:16:33.16 delay 2 seconds @10:16:35.17 ARV.dial :carrier @10:16:35.18 delay 2 seconds @10:16:37.23 AS.seek 0.650ml @10:16:42.12
Killing upper manifold @10:16:42.13 CS Pulling 4ml of mfbkill @10:16:42.14 CSV.dial CSR @10:16:42.64 CSR.select :mfbkill @10:16:42.74 CS.reconfigure CSconfig @10:16:42.78 CS.seek 4.000ml @10:16:53.74 delay 5 seconds @10:16:58.75 CSR.select :air @10:16:58.84 CS.reconfigure CSfast @10:16:58.88 CS.seek 10.000ml @10:17:13.12 CSV.dial CBV @10:17:13.14
CBV.dial CSV @10:17:14.17 CTV.dial :air @10:17:15.46 CS.seek 0.000ml @10:17:28.32 delay 4 seconds @10:17:32.33 ATV.dial PCR @10:17:32.85 ASV.dial :waste1 @10:17:33.77 ARV.dial :air1 @10:17:34.11 Positioning Slug by 0.324ml @10:17:34.16 AS.seek 0.326ml @10:17:38.59
delay 7 seconds @10:17:45.60 CS.seek 3.000ml @10:17:53.19 CSV.dial :waste @10:17:53.62 CS.seek 0.000ml @10:18:01.13 Flushing upper manifold @10:18:01.22 CSV.dial :flush @10:18:01.67 CS.seek 5.000ml @10:18:13.75 delay 5 seconds @10:18:18.76 CSV.dial :air @10:18:19.25 CS.seek 7.000ml @10:18:24.59 CSV.dial CBV @10:18:24.61
CBV.dial CSV @10:18:25.69 CTV.dial :air @10:18:25.71 CS.seek 0.000ml @10:18:42.16 delay 7 seconds @10:18:49.17 CS.seek 3.000ml @10:18:56.72 CSV.dial :waste @10:18:57.23 CS.seek 0.000ml @10:19:05.02 CSV.dial :flush @10:19:05.46 CS.seek 3.000ml @10:19:13.06 delay 5 seconds @10:19:18.07 CSV.dial :air @10:19:18.55 CS.seek 5.000ml @10:19:24.00 CSV.dial :air @10:19:24.02 CS.seek 0.000ml @10:19:36.00 delay 7 seconds @10:19:43.01 CS.seek 3.000ml @10:19:50.62 CSV.dial :waste @10:19:51.41 CS.seek 0.000ml @10:19:58.90 Flushing lower manifold's waste1 port @10:19:58.99 CSV.dial :flush @10:19:59.51 CS.seek 5.000ml @10:20:11.53 delay 5 seconds @10:20:16.54 CSV.dial :air @10:20:17.01 CS.seek 7.000ml @10:20:22.40 CSV.dial CTV @10:20:22.42
CTV.dial CSV @10:20:23.76 CBV.dial :waste1 @10:20:24.19 CS.seek 0.000ml @10:20:40.68 Flushing lower manifold's waste2b port @10:20:40.77 CSV.dial :flush @10:20:41.58 CS.seek 5.000ml @10:20:53.56 delay 5 seconds @10:20:58.57 CSV.dial :air @10:20:59.12 CS.seek 7.000ml @10:21:04.46 CSV.dial CTV @10:21:04.48
CTV.dial CSV @10:21:05.13 CBV.dial :waste2b @10:21:05.88 CS.seek 0.000ml @10:21:22.34 Disinfecting external Intake with kill @10:21:22.43 CSV.dial CSR @10:21:22.95 CSR.select :kill @10:21:23.05 CS.seek 8.000ml @10:21:41.87 delay 5 seconds @10:21:46.88 CSR.select :air @10:21:46.97 CS.seek 10.000ml @10:21:52.40 CSV.dial CBV @10:21:52.42
CBV.dial CSV @10:21:53.43 CTV.dial :intake @10:21:54.13 Intake.select :open @10:21:54.22 CS.seek 2.000ml @10:22:13.40 Intake.select :close @10:22:13.49 CTV.dial :air @10:22:14.22 CS.reconfigure CSconfig @10:22:14.25 CS.seek 0.000ml @10:22:20.15 CSV.dial CSR @10:22:21.19 CSR.select :flush @10:22:21.29 CS.seek 5.000ml @10:22:34.74 delay 5 seconds @10:22:39.75 CSR.select :air @10:22:39.84 CS.seek 10.000ml @10:22:53.36 CSV.dial CBV @10:22:54.42 CS.seek 0.000ml @10:23:04.82 delay 5 seconds @10:23:09.83 ATV.dial ARV @10:23:10.46 ASV.dial :PCRlock @10:23:11.19 delay 5 seconds @10:23:16.20 Running HF183em ProfileEM60C2 thermocycling @10:23:16.21 PCR.program ProfileEM60C2 @10:23:16.33 ProfileEM60C2 should complete in < 2:11:38 @10:23:16.34 PCR.run 2:49:32.5 @10:23:20.71
Garaging FlushPuck in Collection Clamp @10:23:20.77 Elbow.reconfigure TightElbow @10:23:20.80 Elbow.seek CC @10:23:20.90 Elbow.reconfigure LooseElbow @10:23:20.98 Forearm.seek CC @10:23:21.95 CC.seek sealed @10:23:22.89 Hand.grip @10:23:23.67 CC.seek open @10:23:26.99 Forearm.seek retract @10:23:27.93 Elbow.reconfigure TightElbow @10:23:27.97 Elbow.seek garage @10:23:30.77 Elbow.reconfigure LooseElbow @10:23:30.85 Forearm.reconfigure TightForearm @10:23:30.88 Forearm.seek garage @10:23:32.05 Hand.release @10:23:32.84 Forearm.reconfigure LooseForearm @10:23:32.87 Forearm.seek retract @10:23:33.88 Completed {wqPCR} begun 08:02:06 @10:23:33.91 deferred until MFBPCR finish @10:23:33.92
Waiting for MFBPCR til 08:02PST19-Feb-14 @10:23:33.93 Sleeping until 08:02PST19-Feb-14 @10:23:33.94 GoodNews.email "Then, in tube 2: wqPCR\n/home/esp/esp2local/mission/14feb14sccwrp.rb:63",Subject => "Waiting until 08:02PST19-Feb-14" @12:17:16.04 PCR.off @12:17:16.56 PCR for HF183em completed -- closed /var/log/esp/HF183em14feb1810h323ml.pcr @12:17:16.61 Cleaning PCR @12:17:16.62 AFV.select :carrierSolution @12:17:16.71 delay 2 seconds @12:17:18.72 AS.reconfigure ASconfig @12:17:18.75 AS.seek 0.500ml @12:17:29.16 delay 1 second @12:17:30.17 AFV.select :holdingCoil @12:17:30.26 ASV.dial :waste1 @12:17:30.60 ATV.dial :air3 @12:17:31.55 AS.reconfigure ASslow1 @12:17:31.58 AS.seek 0.510ml @12:17:33.88 delay 2 seconds @12:17:35.89 ATV.dial :bleach @12:17:36.29 AS.seek 0.810ml @12:18:37.72 delay 3 seconds @12:18:40.73 ATV.dial :air3 @12:18:41.08 delay 3 seconds @12:18:44.14 AS.seek 0.835ml @12:18:49.48 delay 3 seconds @12:18:52.49 ATV.dial :bleach @12:18:52.88 AS.seek 0.820ml @12:18:56.17 delay 3 seconds @12:18:59.18 ARV.dial :waste @12:18:59.67 delay 3 seconds @12:19:02.68 ATV.dial :ARV @12:19:03.50 AS.reconfigure ASconfig @12:19:03.53 AS.seek 0.470ml @12:19:24.22 AFV.select :carrierSolution @12:19:24.31 delay 2 seconds @12:19:26.92 AS.seek 1.000ml @12:19:58.15 delay 1 second @12:19:59.16 AFV.select :holdingCoil @12:19:59.25 delay 1 second @12:20:00.26 AS.reconfigure ASfast @12:20:00.29 AS.seek 0.000ml @12:20:28.03 AFV.select :carrierSolution @12:20:28.13 delay 2 seconds @12:20:30.14 AS.reconfigure ASconfig @12:20:30.17 AS.seek 1.000ml @12:21:28.79 delay 1 second @12:21:29.80 AFV.select :holdingCoil @12:21:29.89 delay 1 second @12:21:30.90 AS.reconfigure ASfast @12:21:30.93 AS.seek 0.000ml @12:21:58.68 AFV.select :carrierSolution @12:21:58.78 delay 2 seconds @12:22:00.79 AS.reconfigure ASconfig @12:22:00.82 AS.seek 1.000ml @12:22:59.37 delay 1 second @12:23:00.38 AFV.select :holdingCoil @12:23:00.47 delay 1 second @12:23:01.48 AS.reconfigure ASfast @12:23:01.52 AS.seek 0.000ml @12:23:29.24 ARV.dial between carrier and air1 @12:23:29.65 ATV.dial :air3 @12:23:30.60 AS.reconfigure ASslow1 @12:23:30.63 AS.seek 0.010ml @12:23:32.97 delay 2 seconds @12:23:34.98 ATV.dial :bleach @12:23:35.35 AS.seek 0.810ml @12:26:18.77 delay 3 seconds @12:26:21.78 ATV.dial :air3 @12:26:22.11 delay 3 seconds @12:26:25.17 AS.seek 0.835ml @12:26:30.50 delay 3 seconds @12:26:33.51 ATV.dial :bleach @12:26:33.89 AS.seek 0.820ml @12:26:37.22 delay 3 seconds @12:26:40.23 ATV.dial PCR @12:26:40.57 AS.reconfigure ASconfig @12:26:40.60 AS.seek empty @12:27:28.73 PCR.program :bleachProfile @12:27:28.79 PCR program should complete in < 2:45 @12:27:28.80 PCR.run 10:00 @12:30:30.01 PCR.off @12:30:30.04 AFV.select :carrierSolution @12:30:30.13 delay 2 seconds @12:30:32.14 AS.seek 1.000ml @12:31:30.76 delay 1 second @12:31:31.77 AFV.select :holdingCoil @12:31:31.86 delay 1 second @12:31:32.87 AS.reconfigure ASfast @12:31:32.90 AS.seek 0.000ml @12:32:00.58 delay 3 seconds @12:32:03.59 AFV.select :carrierSolution @12:32:03.68 delay 2 seconds @12:32:05.69 AS.reconfigure ASconfig @12:32:05.72 AS.seek 1.000ml @12:33:04.30 delay 1 second @12:33:05.31 AFV.select :holdingCoil @12:33:05.40 delay 1 second @12:33:06.41 AS.reconfigure ASfast @12:33:06.44 AS.seek 0.000ml @12:33:34.19 delay 3 seconds @12:33:37.20 AFV.select :carrierSolution @12:33:37.29 delay 2 seconds @12:33:39.30 AS.reconfigure ASconfig @12:33:39.33 AS.seek 1.000ml @12:34:37.96 delay 1 second @12:34:38.97 AFV.select :holdingCoil @12:34:39.06 delay 1 second @12:34:40.07 AS.reconfigure ASfast @12:34:40.10 AS.seek 0.000ml @12:35:07.82 delay 3 seconds @12:35:10.83 AFV.select :carrierSolution @12:35:10.92 delay 2 seconds @12:35:12.93 AS.reconfigure ASconfig @12:35:12.96 AS.seek 1.000ml @12:36:11.52 delay 1 second @12:36:12.53 AFV.select :holdingCoil @12:36:12.62 delay 1 second @12:36:13.63 AS.reconfigure ASfast @12:36:13.66 AS.seek 0.000ml @12:36:41.38 delay 3 seconds @12:36:44.39 AFV.select :carrierSolution @12:36:44.48 delay 2 seconds @12:36:46.49 AS.reconfigure ASconfig @12:36:46.52 AS.seek 1.000ml @12:37:45.11 delay 1 second @12:37:46.12 AFV.select :holdingCoil @12:37:46.21 delay 1 second @12:37:47.22 AS.reconfigure ASnorm @12:37:47.25 AS.seek 0.000ml @12:38:38.67 Setting up PCR for ENTap -- writing /var/log/esp/ENTap14feb1812h323ml.pcr @12:38:38.68 Building PCR Slug from ASV with MM1 mix, PP1 primer @12:38:38.69 Priming with carrier ATV.dial ARV @12:38:39.21 ARV.dial :carrier @12:38:39.49 AS.seek 0.500ml @12:39:05.35 AFV.select :carrierSolution @12:39:05.46 delay 2 seconds @12:39:07.47 AS.reconfigure ASconfig @12:39:07.50 AS.seek 1.000ml @12:39:36.92 delay 1 second @12:39:37.93 AFV.select :holdingCoil @12:39:38.03 delay 1 second @12:39:39.04 ATV.dial :waste @12:39:40.31 AS.reconfigure ASnorm @12:39:40.34 AS.seek empty @12:40:31.81 AFV.select :carrierSolution @12:40:31.92 delay 2 seconds @12:40:33.93 AS.reconfigure ASconfig @12:40:33.96 AS.seek 0.500ml @12:41:03.45 delay 1 second @12:41:04.46 AFV.select :holdingCoil @12:41:04.56 Building Slug @12:41:04.57 ATV.dial :ARV @12:41:05.91 delay 2 seconds @12:41:07.92 ARV.dial :air1 @12:41:08.25 delay 2 seconds @12:41:10.31 AS.reconfigure ASslow2 @12:41:10.34 AS.seek 0.506ml @12:41:16.81 delay 2 seconds @12:41:18.82 ARV.dial :MM1 @12:41:19.31 delay 2 seconds @12:41:21.32 Adding MM1 @12:41:21.38 AS.seek 0.524ml @12:41:40.56 delay 25 seconds @12:42:05.57 ARV.dial :PP1 @12:42:05.91 Adding PP1 @12:42:05.92 delay 2 seconds @12:42:07.97 AS.seek 0.530ml @12:42:14.20 delay 10 seconds @12:42:24.21 ARV.dial :air1 @12:42:24.54 adding ARV air @12:42:24.55 delay 2 seconds @12:42:26.60 AS.seek 0.536ml @12:42:32.90 delay 5 seconds @12:42:37.91 ARV.dial :carrier @12:42:38.22 delay 7 seconds @12:42:45.28 AS.seek 0.594ml @12:43:44.71 delay 5 seconds @12:43:49.72 ATV.dial :ASV @12:43:50.04 delay 2 seconds @12:43:52.10 AS.seek 0.600ml @12:43:58.49 delay 4 seconds @12:44:02.50 ATV.dial ARV @12:44:02.82 delay 2 seconds @12:44:04.83 ARV.dial :carrier @12:44:04.84 delay 2 seconds @12:44:06.89 AS.seek 0.650ml @12:44:58.09 delay 4 seconds @12:45:02.10 ATV.dial PCR @12:45:02.60 ASV.dial :waste1 @12:45:02.61 ARV.dial :air1 @12:45:02.96 Positioning Slug by 0.324ml @12:45:03.02 AS.seek 0.326ml @12:50:33.64 delay 5 seconds @12:50:38.65 ATV.dial ARV @12:50:39.17 ASV.dial :PCRlock @12:50:39.65 delay 5 seconds @12:50:44.66 Running ENTap Profile60C thermocycling @12:50:44.67 PCR.program Profile60C @12:50:44.77 Profile60C should complete in < 1:39:08 @12:50:44.78 PCR.run 2:08:55 @14:12:04.95 PCR.off @14:12:05.57 PCR for ENTap completed -- closed /var/log/esp/ENTap14feb1812h323ml.pcr @14:12:05.62 Cleaning PCR @14:12:05.63 AFV.select :carrierSolution @14:12:05.72 delay 2 seconds @14:12:08.31 AS.reconfigure ASconfig @14:12:08.34 AS.seek 0.500ml @14:12:18.79 delay 1 second @14:12:19.80 AFV.select :holdingCoil @14:12:19.89 ASV.dial :waste1 @14:12:20.65 ATV.dial :air3 @14:12:21.62 AS.reconfigure ASslow1 @14:12:21.65 AS.seek 0.510ml @14:12:23.93 delay 2 seconds @14:12:25.94 ATV.dial :bleach @14:12:26.34 AS.seek 0.810ml @14:13:27.78 delay 3 seconds @14:13:30.79 ATV.dial :air3 @14:13:31.13 delay 3 seconds @14:13:34.19 AS.seek 0.835ml @14:13:39.53 delay 3 seconds @14:13:42.54 ATV.dial :bleach @14:13:42.93 AS.seek 0.820ml @14:13:46.22 delay 3 seconds @14:13:49.23 ARV.dial :waste @14:13:49.72 delay 3 seconds @14:13:52.73 ATV.dial :ARV @14:13:53.55 AS.reconfigure ASconfig @14:13:53.58 AS.seek 0.470ml @14:14:14.27 AFV.select :carrierSolution @14:14:14.37 delay 2 seconds @14:14:16.38 AS.seek 1.000ml @14:14:47.61 delay 1 second @14:14:48.62 AFV.select :holdingCoil @14:14:48.71 delay 1 second @14:14:49.72 AS.reconfigure ASfast @14:14:49.75 AS.seek 0.000ml @14:15:17.50 AFV.select :carrierSolution @14:15:17.60 delay 2 seconds @14:15:19.61 AS.reconfigure ASconfig @14:15:19.64 AS.seek 1.000ml @14:16:18.23 delay 1 second @14:16:19.24 AFV.select :holdingCoil @14:16:19.33 delay 1 second @14:16:20.34 AS.reconfigure ASfast @14:16:20.37 AS.seek 0.000ml @14:16:48.10 AFV.select :carrierSolution @14:16:48.20 delay 2 seconds @14:16:50.21 AS.reconfigure ASconfig @14:16:50.24 AS.seek 1.000ml @14:17:48.80 delay 1 second @14:17:49.81 AFV.select :holdingCoil @14:17:49.90 delay 1 second @14:17:50.91 AS.reconfigure ASfast @14:17:50.94 AS.seek 0.000ml @14:18:18.64 ARV.dial between carrier and air1 @14:18:19.16 ATV.dial :air3 @14:18:20.10 AS.reconfigure ASslow1 @14:18:20.13 AS.seek 0.010ml @14:18:22.44 delay 2 seconds @14:18:24.45 ATV.dial :bleach @14:18:24.93 AS.seek 0.810ml @14:21:08.30 delay 3 seconds @14:21:11.31 ATV.dial :air3 @14:21:11.64 delay 3 seconds @14:21:14.70 AS.seek 0.835ml @14:21:20.03 delay 3 seconds @14:21:23.04 ATV.dial :bleach @14:21:23.44 AS.seek 0.820ml @14:21:26.76 delay 3 seconds @14:21:29.77 ATV.dial PCR @14:21:30.09 AS.reconfigure ASconfig @14:21:30.12 AS.seek empty @14:22:18.25 PCR.program :bleachProfile @14:22:18.31 PCR program should complete in < 2:45 @14:22:18.32 PCR.run 10:00 @14:25:19.53 PCR.off @14:25:19.56 AFV.select :carrierSolution @14:25:19.65 delay 2 seconds @14:25:21.66 AS.seek 1.000ml @14:26:20.30 delay 1 second @14:26:21.31 AFV.select :holdingCoil @14:26:21.40 delay 1 second @14:26:22.41 AS.reconfigure ASfast @14:26:22.44 AS.seek 0.000ml @14:26:50.14 delay 3 seconds @14:26:53.15 AFV.select :carrierSolution @14:26:53.24 delay 2 seconds @14:26:55.25 AS.reconfigure ASconfig @14:26:55.28 AS.seek 1.000ml @14:27:53.88 delay 1 second @14:27:54.89 AFV.select :holdingCoil @14:27:54.98 delay 1 second @14:27:55.99 AS.reconfigure ASfast @14:27:56.02 AS.seek 0.000ml @14:28:23.70 delay 3 seconds @14:28:26.71 AFV.select :carrierSolution @14:28:26.80 delay 2 seconds @14:28:28.81 AS.reconfigure ASconfig @14:28:28.84 AS.seek 1.000ml @14:29:27.42 delay 1 second @14:29:28.43 AFV.select :holdingCoil @14:29:28.52 delay 1 second @14:29:29.53 AS.reconfigure ASfast @14:29:29.56 AS.seek 0.000ml @14:29:57.25 delay 3 seconds @14:30:00.26 AFV.select :carrierSolution @14:30:00.35 delay 2 seconds @14:30:02.36 AS.reconfigure ASconfig @14:30:02.39 AS.seek 1.000ml @14:31:00.95 delay 1 second @14:31:01.96 AFV.select :holdingCoil @14:31:02.05 delay 1 second @14:31:03.06 AS.reconfigure ASfast @14:31:03.09 AS.seek 0.000ml @14:31:30.82 delay 3 seconds @14:31:33.83 AFV.select :carrierSolution @14:31:33.92 delay 2 seconds @14:31:35.93 AS.reconfigure ASconfig @14:31:35.96 AS.seek 1.000ml @14:32:34.51 delay 1 second @14:32:35.52 AFV.select :holdingCoil @14:32:35.61 delay 1 second @14:32:36.62 AS.reconfigure ASnorm @14:32:36.65 AS.seek 0.000ml @14:33:28.12 Setting up PCR for ENTem -- writing /var/log/esp/ENTem14feb1814h323ml.pcr @14:33:28.13 Building PCR Slug from ASV with MM2 mix, PP3 primer @14:33:28.14 Priming with carrier @14:33:28.15 ATV.dial ARV @14:33:28.66 ARV.dial :carrier @14:33:28.93 AS.seek 0.500ml @14:33:55.28 AFV.select :carrierSolution @14:33:55.48 delay 2 seconds @14:33:57.49 AS.reconfigure ASconfig @14:33:57.53 AS.seek 1.000ml @14:34:26.97 delay 1 second @14:34:27.98 AFV.select :holdingCoil @14:34:28.07 delay 1 second @14:34:29.08 ATV.dial :waste @14:34:30.35 AS.reconfigure ASnorm @14:34:30.38 AS.seek empty @14:35:21.86 AFV.select :carrierSolution @14:35:21.97 delay 2 seconds @14:35:23.98 AS.reconfigure ASconfig @14:35:24.01 AS.seek 0.500ml @14:35:53.48 delay 1 second @14:35:54.49 AFV.select :holdingCoil @14:35:54.59 Building Slug @14:35:54.60 ATV.dial :ARV @14:35:55.87 delay 2 seconds @14:35:57.88 ARV.dial :air1 @14:35:58.20 delay 2 seconds @14:36:00.26 AS.reconfigure ASslow2 @14:36:00.29 AS.seek 0.506ml @14:36:06.72 delay 2 seconds @14:36:08.73 ARV.dial :MM2 @14:36:09.95 delay 2 seconds @14:36:11.96 Adding MM2 @14:36:12.02 AS.seek 0.524ml @14:36:30.56 delay 25 seconds @14:36:55.57 ARV.dial :PP3 @14:36:55.99 Adding PP3 @14:36:56.00 delay 2 seconds @14:36:58.05 AS.seek 0.530ml @14:37:04.29 delay 10 seconds @14:37:14.30 ARV.dial :air2 @14:37:14.63 adding ARV air @14:37:14.64 delay 2 seconds @14:37:16.69 AS.seek 0.536ml @14:37:23.04 delay 5 seconds @14:37:28.05 ARV.dial :carrier @14:37:29.09 delay 7 seconds @14:37:36.15 AS.seek 0.594ml @14:38:35.77 delay 5 seconds @14:38:40.78 ATV.dial :ASV @14:38:41.10 delay 2 seconds @14:38:43.16 AS.seek 0.600ml @14:38:49.46 delay 4 seconds @14:38:53.47 ATV.dial ARV @14:38:53.91 delay 2 seconds @14:38:55.92 ARV.dial :carrier @14:38:55.93 delay 2 seconds @14:38:57.98 AS.seek 0.650ml @14:39:49.08 delay 4 seconds @14:39:53.09 ATV.dial PCR @14:39:53.60 ASV.dial :waste1 @14:39:53.61 ARV.dial :air1 @14:39:54.05 Positioning Slug by 0.324ml @14:39:54.11 AS.seek 0.326ml @14:45:24.90 delay 5 seconds @14:45:29.91 ATV.dial ARV @14:45:30.43 ASV.dial :PCRlock @14:45:30.76 delay 5 seconds @14:45:35.77 Running ENTem ProfileEM60C thermocycling @14:45:35.78 PCR.program ProfileEM60C @14:45:35.89 ProfileEM60C should complete in < 2:11:38 @14:45:35.90 PCR.run 2:49:32.5 @16:39:53.26 PCR.off @16:39:53.80 PCR for ENTem completed -- closed /var/log/esp/ENTem14feb1814h323ml.pcr @16:39:53.85 Cleaning PCR AFV.select :carrierSolution @16:39:53.94 delay 2 seconds @16:39:55.95 AS.reconfigure ASconfig @16:39:55.98 AS.seek 0.500ml @16:40:06.40 delay 1 second @16:40:07.41 AFV.select :holdingCoil @16:40:07.50 ASV.dial :waste1 @16:40:07.99 ATV.dial :air3 @16:40:08.93 AS.reconfigure ASslow1 @16:40:08.96 AS.seek 0.510ml @16:40:11.24 delay 2 seconds @16:40:13.25 ATV.dial :bleach @16:40:13.74 AS.seek 0.810ml @16:41:15.16 delay 3 seconds @16:41:18.17 ATV.dial :air3 @16:41:18.50 delay 3 seconds @16:41:21.56 AS.seek 0.835ml @16:41:26.91 delay 3 seconds @16:41:29.92 ATV.dial :bleach @16:41:30.32 AS.seek 0.820ml @16:41:33.63 delay 3 seconds @16:41:36.64 ARV.dial :waste @16:41:37.14 delay 3 seconds @16:41:40.15 ATV.dial :ARV @16:41:40.88 AS.reconfigure ASconfig @16:41:40.91 AS.seek 0.470ml @16:42:01.58 AFV.select :carrierSolution @16:42:01.68 delay 2 seconds @16:42:03.69 AS.seek 1.000ml @16:42:34.88 delay 1 second @16:42:35.89 AFV.select :holdingCoil @16:42:35.98 delay 1 second @16:42:36.99 AS.reconfigure ASfast @16:42:37.02 AS.seek 0.000ml @16:43:04.72 AFV.select :carrierSolution @16:43:04.82 delay 2 seconds @16:43:06.83 AS.reconfigure ASconfig @16:43:06.86 AS.seek 1.000ml @16:44:05.47 delay 1 second @16:44:06.48 AFV.select :holdingCoil @16:44:06.57 delay 1 second @16:44:07.58 AS.reconfigure ASfast @16:44:07.61 AS.seek 0.000ml @16:44:35.34 AFV.select :carrierSolution @16:44:35.44 delay 2 seconds @16:44:37.45 AS.reconfigure ASconfig @16:44:37.48 AS.seek 1.000ml @16:45:36.09 delay 1 second @16:45:37.10 AFV.select :holdingCoil @16:45:37.19 delay 1 second @16:45:38.20 AS.reconfigure ASfast @16:45:38.23 AS.seek 0.000ml @16:46:05.96 ARV.dial between carrier and air1 @16:46:06.48 ATV.dial :air3 @16:46:07.42 AS.reconfigure ASslow1 @16:46:07.45 AS.seek 0.010ml @16:46:09.78 delay 2 seconds @16:46:11.79 ATV.dial :bleach @16:46:12.19 AS.seek 0.810ml @16:48:55.57 delay 3 seconds @16:48:58.58 ATV.dial :air3 @16:48:58.93 delay 3 seconds @16:49:01.99 AS.seek 0.835ml @16:49:07.33 delay 3 seconds @16:49:10.34 ATV.dial :bleach @16:49:10.73 AS.seek 0.820ml @16:49:14.02 delay 3 seconds @16:49:17.03 ATV.dial PCR @16:49:17.36 AS.reconfigure ASconfig @16:49:17.39 AS.seek empty @16:50:05.51 PCR.program :bleachProfile @16:50:05.57 PCR program should complete in < 2:45 @16:50:05.58 PCR.run 10:00 @16:53:06.79 PCR.off @16:53:06.82 AFV.select :carrierSolution @16:53:06.91 delay 2 seconds @16:53:08.92 AS.seek 1.000ml @16:54:07.52 delay 1 second @16:54:08.53 AFV.select :holdingCoil @16:54:08.62 delay 1 second @16:54:09.63 AS.reconfigure ASfast @16:54:09.66 AS.seek 0.000ml @16:54:37.36 delay 3 seconds @16:54:40.37 AFV.select :carrierSolution @16:54:40.46 delay 2 seconds @16:54:42.47 AS.reconfigure ASconfig @16:54:42.50 AS.seek 1.000ml @16:55:41.10 delay 1 second @16:55:42.11 AFV.select :holdingCoil @16:55:42.20 delay 1 second @16:55:43.21 AS.reconfigure ASfast @16:55:43.24 AS.seek 0.000ml @16:56:10.97 delay 3 seconds @16:56:13.98 AFV.select :carrierSolution @16:56:14.07 delay 2 seconds @16:56:16.08 AS.reconfigure ASconfig @16:56:16.11 AS.seek 1.000ml @16:57:14.70 delay 1 second @16:57:15.71 AFV.select :holdingCoil @16:57:15.80 delay 1 second @16:57:16.81 AS.reconfigure ASfast @16:57:16.85 AS.seek 0.000ml @16:57:44.56 delay 3 seconds @16:57:47.57 AFV.select :carrierSolution @16:57:47.66 delay 2 seconds @16:57:49.67 AS.reconfigure ASconfig @16:57:49.70 AS.seek 1.000ml @16:58:48.29 delay 1 second @16:58:49.30 AFV.select :holdingCoil @16:58:49.39 delay 1 second @16:58:50.40 AS.reconfigure ASfast @16:58:50.43 AS.seek 0.000ml @16:59:18.14 delay 3 seconds @16:59:21.15 AFV.select :carrierSolution @16:59:21.24 delay 2 seconds @16:59:23.25 AS.reconfigure ASconfig @16:59:23.28 AS.seek 1.000ml @17:00:21.85 delay 1 second @17:00:22.86 AFV.select :holdingCoil @17:00:22.95 delay 1 second @17:00:23.96 AS.reconfigure ASnorm @17:00:23.99 AS.seek 0.000ml @17:01:15.46 Setting up PCR for IPCap -- writing /var/log/esp/IPCap14feb1817h323ml.pcr @17:01:15.47 Building PCR Slug from ASV with MM1 mix, PP2 primer @17:01:15.48 Priming with carrier @17:01:15.49 ATV.dial ARV @17:01:16.01 ARV.dial :carrier @17:01:16.28 AS.seek 0.500ml @17:01:42.18 AFV.select :carrierSolution @17:01:42.29 delay 2 seconds @17:01:44.30 AS.reconfigure ASconfig @17:01:44.33 AS.seek 1.000ml @17:02:13.77 delay 1 second @17:02:14.78 AFV.select :holdingCoil @17:02:14.87 delay 1 second @17:02:15.88 ATV.dial :waste @17:02:17.16 AS.reconfigure ASnorm @17:02:17.19 AS.seek empty @17:03:08.63 AFV.select :carrierSolution @17:03:08.74 delay 2 seconds @17:03:10.75 AS.reconfigure ASconfig @17:03:10.78 AS.seek 0.500ml @17:03:40.27 delay 1 second @17:03:41.28 AFV.select :holdingCoil @17:03:41.38 Building Slug @17:03:41.39 ATV.dial :ARV @17:03:42.66 delay 2 seconds @17:03:44.67 ARV.dial :air1 @17:03:45.00 delay 2 seconds @17:03:47.06 AS.reconfigure ASslow2 @17:03:47.09 AS.seek 0.506ml @17:03:53.55 delay 2 seconds @17:03:55.56 ARV.dial :MM1 @17:03:56.14 delay 2 seconds @17:03:58.15 Adding MM1 @17:03:58.21 AS.seek 0.524ml @17:04:16.72 delay 25 seconds @17:04:41.73 ARV.dial :PP2 @17:04:42.06 Adding PP2 @17:04:42.08 delay 2 seconds @17:04:44.13 AS.seek 0.530ml @17:04:50.52 delay 10 seconds @17:05:00.53 ARV.dial :air2 @17:05:00.94 adding ARV air @17:05:00.95 delay 2 seconds @17:05:03.01 AS.seek 0.536ml @17:05:09.24 delay 5 seconds @17:05:14.25 ARV.dial :carrier @17:05:15.28 delay 7 seconds @17:05:22.34 AS.seek 0.594ml @17:06:21.67 delay 5 seconds @17:06:26.68 ATV.dial :ASV @17:06:27.01 delay 2 seconds @17:06:29.07 AS.seek 0.600ml @17:06:35.69 delay 4 seconds @17:06:39.70 ATV.dial ARV @17:06:40.14 delay 2 seconds @17:06:42.15 ARV.dial :carrier @17:06:42.16 delay 2 seconds @17:06:44.21 AS.seek 0.650ml @17:07:35.62 delay 4 seconds @17:07:39.63 ATV.dial PCR @17:07:40.15 ASV.dial :waste1 @17:07:40.16 ARV.dial :air1 @17:07:40.50 Positioning Slug by 0.324ml @17:07:40.56 AS.seek 0.326ml @17:13:11.11 delay 5 seconds @17:13:16.12 ATV.dial ARV @17:13:16.64 ASV.dial :PCRlock @17:13:17.14 delay 5 seconds @17:13:22.15 Running IPCap Profile59C thermocycling @17:13:22.16 PCR.program Profile59C @17:13:22.26 Profile59C should complete in < 1:39:08 @17:13:22.27 PCR.run 2:08:55 @18:35:07.18 PCR.off @18:35:07.72 PCR for IPCap completed -- closed /var/log/esp/IPCap14feb1817h323ml.pcr @18:35:07.76 Cleaning PCR @18:35:07.77 AFV.select :carrierSolution @18:35:07.86 delay 2 seconds @18:35:09.87 AS.reconfigure ASconfig @18:35:09.90 AS.seek 0.500ml @18:35:20.36 delay 1 second @18:35:21.37 AFV.select :holdingCoil @18:35:21.46 ASV.dial :waste1 @18:35:21.94 ATV.dial :air3 @18:35:22.98 AS.reconfigure ASslow1 @18:35:23.01 AS.seek 0.510ml @18:35:25.30 delay 2 seconds @18:35:27.31 ATV.dial :bleach @18:35:27.68 AS.seek 0.810ml @18:36:29.11 delay 3 seconds @18:36:32.12 ATV.dial :air3 @18:36:32.46 delay 3 seconds @18:36:35.52 AS.seek 0.835ml @18:36:40.86 delay 3 seconds @18:36:43.87 ATV.dial :bleach @18:36:44.25 AS.seek 0.820ml @18:36:47.58 delay 3 seconds @18:36:50.59 ARV.dial :waste @18:36:51.19 delay 3 seconds @18:36:54.20 ATV.dial :ARV @18:36:54.96 AS.reconfigure ASconfig @18:36:54.99 AS.seek 0.470ml @18:37:15.69 AFV.select :carrierSolution @18:37:15.78 delay 2 seconds @18:37:17.79 AS.seek 1.000ml @18:37:48.98 delay 1 second @18:37:49.99 AFV.select :holdingCoil @18:37:50.08 delay 1 second @18:37:51.09 AS.reconfigure ASfast @18:37:51.12 AS.seek 0.000ml @18:38:18.86 AFV.select :carrierSolution @18:38:18.96 delay 2 seconds @18:38:20.97 AS.reconfigure ASconfig @18:38:21.00 AS.seek 1.000ml @18:39:19.61 delay 1 second @18:39:20.62 AFV.select :holdingCoil @18:39:20.71 delay 1 second @18:39:21.72 AS.reconfigure ASfast @18:39:21.75 AS.seek 0.000ml @18:39:49.48 AFV.select :carrierSolution @18:39:49.58 delay 2 seconds @18:39:51.59 AS.reconfigure ASconfig @18:39:51.62 AS.seek 1.000ml @18:40:50.23 delay 1 second @18:40:51.24 AFV.select :holdingCoil @18:40:51.33 delay 1 second @18:40:52.34 AS.reconfigure ASfast @18:40:52.37 AS.seek 0.000ml @18:41:20.09 ARV.dial between carrier and air1 @18:41:20.51 ATV.dial :air3 @18:41:21.54 AS.reconfigure ASslow1 @18:41:21.58 AS.seek 0.010ml @18:41:23.95 delay 2 seconds @18:41:25.96 ATV.dial :bleach @18:41:26.34 AS.seek 0.810ml @18:44:09.74 delay 3 seconds @18:44:12.75 ATV.dial :air3 @18:44:13.08 delay 3 seconds @18:44:16.14 AS.seek 0.835ml @18:44:21.47 delay 3 seconds @18:44:24.48 ATV.dial :bleach @18:44:24.85 AS.seek 0.820ml @18:44:28.80 delay 3 seconds @18:44:31.81 ATV.dial PCR @18:44:32.15 AS.reconfigure ASconfig @18:44:32.18 AS.seek empty @18:45:20.31 PCR.program :bleachProfile @18:45:20.37 PCR program should complete in < 2:45 @18:45:20.38 PCR.run 10:00 @18:48:21.59 PCR.off @18:48:21.62 AFV.select :carrierSolution @18:48:21.71 delay 2 seconds @18:48:23.72 AS.seek 1.000ml @18:49:22.36 delay 1 second @18:49:23.37 AFV.select :holdingCoil @18:49:23.46 delay 1 second @18:49:24.47 AS.reconfigure ASfast @18:49:24.50 AS.seek 0.000ml @18:49:52.22 delay 3 seconds @18:49:55.23 AFV.select :carrierSolution @18:49:55.32 delay 2 seconds @18:49:57.33 AS.reconfigure ASconfig @18:49:57.36 AS.seek 1.000ml @18:50:55.94 delay 1 second @18:50:56.95 AFV.select :holdingCoil @18:50:57.08 delay 1 second @18:50:58.09 AS.reconfigure ASfast @18:50:58.12 AS.seek 0.000ml @18:51:25.83 delay 3 seconds @18:51:28.84 AFV.select :carrierSolution @18:51:28.93 delay 2 seconds @18:51:30.94 AS.reconfigure ASconfig @18:51:30.97 AS.seek 1.000ml @18:52:29.54 delay 1 second @18:52:30.55 AFV.select :holdingCoil @18:52:30.64 delay 1 second @18:52:31.65 AS.reconfigure ASfast @18:52:31.68 AS.seek 0.000ml @18:52:59.40 delay 3 seconds @18:53:02.41 AFV.select :carrierSolution @18:53:02.50 delay 2 seconds @18:53:04.51 AS.reconfigure ASconfig @18:53:04.54 AS.seek 1.000ml @18:54:03.14 delay 1 second @18:54:04.15 AFV.select :holdingCoil @18:54:04.24 delay 1 second @18:54:05.25 AS.reconfigure ASfast @18:54:05.28 AS.seek 0.000ml @18:54:33.01 delay 3 seconds @18:54:36.02 AFV.select :carrierSolution @18:54:36.11 delay 2 seconds @18:54:38.12 AS.reconfigure ASconfig @18:54:38.15 AS.seek 1.000ml @18:55:36.74 delay 1 second @18:55:37.75 AFV.select :holdingCoil @18:55:37.84 delay 1 second @18:55:38.85 AS.reconfigure ASnorm @18:55:38.88 AS.seek 0.000ml @18:56:30.33 Setting up PCR for IPCem -- writing /var/log/esp/IPCem14feb1818h323ml.pcr @18:56:30.34 Building PCR Slug from ASV with MM3 mix, PP5 primer @18:56:30.35 Priming with carrier ATV.dial ARV @18:56:30.87 ARV.dial :carrier @18:56:31.17 AS.seek 0.500ml @18:56:57.05 AFV.select :carrierSolution @18:56:57.14 delay 2 seconds @18:56:59.15 AS.reconfigure ASconfig @18:56:59.18 AS.seek 1.000ml @18:57:28.61 delay 1 second @18:57:29.62 AFV.select :holdingCoil @18:57:29.72 delay 1 second @18:57:30.73 ATV.dial :waste @18:57:32.00 AS.reconfigure ASnorm @18:57:32.03 AS.seek empty @18:58:23.50 AFV.select :carrierSolution @18:58:23.61 delay 2 seconds @18:58:25.62 AS.reconfigure ASconfig @18:58:25.65 AS.seek 0.500ml @18:58:55.14 delay 1 second @18:58:56.15 AFV.select :holdingCoil @18:58:56.25 Building Slug @18:58:56.26 ATV.dial :ARV @18:58:57.60 delay 2 seconds @18:58:59.61 ARV.dial :air1 @18:58:59.94 delay 2 seconds @18:59:02.00 AS.reconfigure ASslow2 @18:59:02.03 AS.seek 0.506ml @18:59:08.39 delay 2 seconds @18:59:10.40 ARV.dial :MM3 @18:59:11.25 delay 2 seconds @18:59:13.26 Adding MM3 @18:59:13.32 AS.seek 0.524ml @18:59:32.06 delay 25 seconds @18:59:57.07 ARV.dial :PP5 @18:59:57.39 Adding PP5 @18:59:57.41 delay 2 seconds @18:59:59.46 AS.seek 0.530ml @19:00:05.91 delay 10 seconds @19:00:15.92 ARV.dial :air3 @19:00:16.23 adding ARV air @19:00:16.24 delay 2 seconds @19:00:18.30 AS.seek 0.536ml @19:00:24.58 delay 5 seconds @19:00:29.59 ARV.dial :carrier @19:00:30.62 delay 7 seconds @19:00:37.68 AS.seek 0.594ml @19:01:37.01 delay 5 seconds @19:01:42.02 ATV.dial :ASV @19:01:42.34 delay 2 seconds @19:01:44.40 AS.seek 0.600ml @19:01:50.67 delay 4 seconds @19:01:54.68 ATV.dial ARV @19:01:55.01 delay 2 seconds @19:01:57.02 ARV.dial :carrier @19:01:57.03 delay 2 seconds @19:01:59.08 AS.seek 0.650ml @19:02:50.39 delay 4 seconds @19:02:54.40 ATV.dial PCR @19:02:55.01 ASV.dial :waste1 @19:02:55.02 ARV.dial :air1 @19:02:55.36 Positioning Slug by 0.324ml @19:02:55.41 AS.seek 0.326ml @19:08:26.14 delay 5 seconds @19:08:31.15 ATV.dial ARV @19:08:32.32 ASV.dial :PCRlock @19:08:32.81 delay 5 seconds @19:08:37.82 Running IPCem ProfileEM59C thermocycling @19:08:37.83 PCR.program ProfileEM59C @19:08:37.94 ProfileEM59C should complete in < 2:11:38 @19:08:37.95 PCR.run 2:49:32.5 @21:03:04.08 PCR.off @21:03:04.61 PCR for IPCem completed -- closed /var/log/esp/IPCem14feb1818h323ml.pcr @21:03:04.63 Full MFB Decontamination Decon extraction column and decon AIV @21:03:04.65 SPE.hold 2:46:40,55.0C @21:03:04.66 ASV.dial :SPEdelivery @21:03:05.61 delay 20 seconds @21:03:25.62 AFV.select :carrierSolution @21:03:25.71 delay 2 seconds @21:03:27.72 AS.reconfigure ASconfig @21:03:27.75 AS.seek 0.500ml @21:03:38.19 delay 1 second @21:03:39.20 AFV.select :holdingCoil @21:03:39.29 ATV.dial :air1 @21:03:40.82 AS.reconfigure ASslow1 @21:03:40.85 AS.seek 0.520ml @21:03:45.20 ATV.dial :ASV @21:03:46.48 ASV.dial :SPErecovery @21:03:47.19 AS.seek 0.620ml @21:04:07.81 ATV.dial :waste @21:04:09.27 AS.reconfigure ASnorm @21:04:09.30 AS.seek empty @21:04:41.34 AFV.select :holdingCoil @21:04:41.35 ATV.dial :waste @21:04:41.36 AS.reconfigure ASconfig @21:04:41.39 AS.seek 0.000ml @21:04:41.69 AFV.select :carrierSolution @21:04:41.80 delay 2 seconds @21:04:43.81 AS.seek 0.500ml @21:05:13.22 delay 1 second @21:05:14.23 AFV.select :holdingCoil @21:05:14.32 ATV.dial :air3 @21:05:14.89 AS.reconfigure ASslow1 @21:05:14.92 AS.seek 0.520ml @21:05:19.77 ATV.dial :air3 @21:05:19.83 AS.seek 0.530ml @21:05:22.12 delay 2 seconds @21:05:24.13 ATV.dial :bleach @21:05:24.50 AS.seek 0.710ml @21:06:01.46 delay 3 seconds @21:06:04.47 ATV.dial :air3 @21:06:04.80 delay 3 seconds @21:06:07.86 AS.seek 0.735ml @21:06:13.19 delay 3 seconds @21:06:16.20 ATV.dial :bleach @21:06:16.69 AS.seek 0.720ml @21:06:20.02 delay 3 seconds @21:06:23.03 ATV.dial ASV @21:06:24.04 ASV.dial :SPErecovery @21:06:24.10 AS.seek 0.365ml @21:07:36.72 ATV.dial :waste @21:07:38.20 AS.reconfigure ASnorm @21:07:38.23 AS.seek empty @21:07:57.24 ATV.dial :air3 @21:07:57.82 AS.reconfigure ASslow1 @21:07:57.85 AS.seek 0.009ml @21:08:00.19 delay 2 seconds @21:08:02.20 ATV.dial :bleach @21:08:02.58 AS.seek 0.579ml @21:09:59.06 delay 3 seconds @21:10:02.07 ATV.dial :air3 @21:10:02.42 delay 3 seconds @21:10:05.48 AS.seek 0.604ml @21:10:10.86 delay 3 seconds @21:10:13.87 ATV.dial :bleach @21:10:14.27 AS.seek 0.589ml @21:10:17.56 delay 3 seconds @21:10:20.57 ATV.dial :ASV @21:10:21.47 ASV.dial :wasteR @21:10:22.24 AS.seek 0.000ml @21:12:22.62 delay 1:00 @21:13:22.63 ATV.dial ASV @21:13:22.64 AFV.select :carrierSolution @21:13:22.74 delay 2 seconds @21:13:24.75 AS.reconfigure ASconfig @21:13:24.78 AS.seek 1.000ml @21:14:23.38 delay 1 second @21:14:24.39 AFV.select :holdingCoil @21:14:24.48 delay 1 second @21:14:25.49 ASV.dial :wasteR @21:14:25.50 AS.reconfigure ASslow1 @21:14:25.53 AS.seek 0.000ml @21:17:49.80 delay 10 seconds @21:17:59.81 ATV.dial :air3 @21:18:00.94 AS.seek 0.010ml @21:18:03.26 delay 2 seconds @21:18:05.27 ATV.dial :bleach @21:18:05.64 AS.seek 0.580ml @21:20:02.17 delay 3 seconds @21:20:05.18 ATV.dial :air3 @21:20:05.51 delay 3 seconds @21:20:08.57 AS.seek 0.605ml @21:20:13.93 delay 3 seconds @21:20:16.94 ATV.dial :bleach @21:20:17.31 AS.seek 0.590ml @21:20:20.62 delay 3 seconds @21:20:23.63 ATV.dial ASV @21:20:24.62 ASV.dial :SPEdelivery @21:20:25.10 AS.seek 0.000ml @21:22:25.68 delay 1:00 @21:23:25.70 AFV.select :carrierSolution @21:23:25.80 delay 2 seconds @21:23:27.81 AS.reconfigure ASconfig @21:23:27.84 AS.seek 1.000ml @21:24:26.39 delay 1 second @21:24:27.40 AFV.select :holdingCoil @21:24:27.50 delay 1 second @21:24:28.51 ATV.dial ASV @21:24:28.52 ASV.dial :SPEdelivery @21:24:28.53 AS.reconfigure ASslow1 @21:24:28.56 AS.seek 0.000ml @21:27:52.80 delay 10 seconds @21:28:02.81 ATV.dial :air2 @21:28:03.64 AS.reconfigure ASslow0 @21:28:03.67 AS.seek 0.800ml @21:29:25.55 delay 3 seconds @21:29:28.56 ATV.dial :ASV @21:29:29.27 ASV.dial :SPEdelivery @21:29:29.29 AS.reconfigure ASslow1 @21:29:29.32 AS.seek 0.030ml @21:32:06.64 delay 20 seconds @21:32:26.65 ATV.dial :waste,via => air3 @21:32:28.10 AS.reconfigure ASconfig @21:32:28.13 AS.seek empty @21:32:30.22 SPE.coast @21:32:30.30 Removing Bleach from Mixing Coil @21:32:30.31 AFV.select :carrierSolution @21:32:30.40 delay 2 seconds @21:32:32.41 AS.seek 0.500ml @21:33:01.89 delay 1 second @21:33:02.90 AFV.select :holdingCoil @21:33:02.99 ATV.dial :ASV @21:33:04.45 ASV.dial :SPErecovery @21:33:05.41 AS.reconfigure ASslow1 @21:33:05.45 AS.seek 0.730ml @21:33:52.60 ASV.dial :wasteD @21:33:53.27 AS.reconfigure ASnorm @21:33:53.30 AS.seek 0.000ml @21:34:30.90 AFV.select :carrierSolution @21:34:30.99 delay 2 seconds @21:34:33.00 AS.reconfigure ASconfig @21:34:33.03 AS.seek 0.500ml @21:35:02.46 delay 1 second @21:35:03.47 AFV.select :holdingCoil @21:35:03.56 ATV.dial ASV @21:35:03.57 ASV.dial :SPErecovery @21:35:04.28 AS.reconfigure ASslow0 @21:35:04.31 AS.seek 0.300ml @21:35:24.98 ATV.dial :air2 @21:35:25.75 AS.reconfigure ASslow1 @21:35:25.78 AS.seek 0.320ml @21:35:30.16 ATV.dial ASV @21:35:30.93 AS.reconfigure ASslow0 @21:35:30.96 AS.seek 0.570ml @21:35:56.77 ASV.dial :wasteD @21:35:58.02 AS.reconfigure ASnorm @21:35:58.05 AS.seek 0.000ml @21:36:27.49 AFV.select :carrierSolution @21:36:27.60 delay 2 seconds @21:36:29.61 AS.reconfigure ASconfig @21:36:29.64 AS.seek 0.500ml @21:36:59.07 delay 1 second @21:37:00.08 AFV.select :holdingCoil @21:37:00.18 ATV.dial ASV @21:37:00.19 ASV.dial :SPErecovery @21:37:00.90 AS.reconfigure ASslow0 @21:37:00.93 AS.seek 0.300ml @21:37:21.63 ATV.dial :air2 @21:37:22.40 AS.reconfigure ASslow1 @21:37:22.43 AS.seek 0.320ml @21:37:26.77 ATV.dial ASV @21:37:27.63 AS.reconfigure ASslow0 @21:37:27.66 AS.seek 0.570ml @21:37:53.44 ASV.dial :wasteD @21:37:54.11 AS.reconfigure ASnorm @21:37:54.14 AS.seek 0.000ml @21:38:23.61 ATV.dial :air2 @21:38:24.42 AS.reconfigure ASslow0 @21:38:24.45 AS.seek 0.300ml @21:38:55.33 ATV.dial :ASV @21:38:56.13 ASV.dial :wasteD @21:38:56.15 AS.reconfigure ASconfig @21:38:56.18 AS.seek 0.050ml @21:39:11.03 ATV.dial :waste @21:39:12.59 AS.seek 0.000ml @21:39:15.78 ASV.dial :SPEdelivery @21:39:16.80 deconLysateINT @21:39:16.81 ATV.dial :air3 @21:39:17.39 AS.reconfigure ASslow1 @21:39:17.42 AS.seek 0.010ml @21:39:19.75 delay 2 seconds @21:39:21.76 ATV.dial :bleach @21:39:22.14 AS.seek 0.960ml @21:42:36.15 delay 3 seconds @21:42:39.16 ATV.dial :air3 @21:42:39.50 delay 3 seconds @21:42:42.56 AS.seek 0.985ml @21:42:47.89 delay 3 seconds @21:42:50.90 ATV.dial :bleach @21:42:51.38 AS.seek 0.970ml @21:42:54.71 delay 3 seconds @21:42:57.72 ATV.dial :AIV @21:42:58.82 AIV.dial :coilToMFB @21:42:58.83 AWV.select :waste @21:42:58.84 AFV.select :carrierSolution @21:42:58.93 delay 2 seconds @21:43:00.94 AS.reconfigure ASconfig @21:43:00.97 AS.seek 1.000ml @21:43:03.03 delay 1 second @21:43:04.04 AFV.select :holdingCoil @21:43:04.13 delay 1 second @21:43:05.14 AS.reconfigure ASslow0 @21:43:05.17 AS.seek empty @21:44:47.58 AFV.select :carrierSolution @21:44:47.68 delay 2 seconds @21:44:49.69 AS.reconfigure ASconfig @21:44:49.72 AS.seek 1.000ml @21:45:48.33 delay 1 second @21:45:49.34 AFV.select :holdingCoil @21:45:49.43 delay 1 second @21:45:50.44 AS.reconfigure ASslow0 @21:45:50.47 AS.seek empty @21:47:32.89 AFV.select :carrierSolution @21:47:32.99 delay 2 seconds @21:47:35.00 AS.reconfigure ASconfig @21:47:35.03 AS.seek 1.000ml @21:48:33.65 delay 1 second @21:48:34.66 AFV.select :holdingCoil @21:48:34.76 delay 1 second @21:48:35.77 AS.reconfigure ASslow0 @21:48:35.80 AS.seek empty @21:50:18.24 ATV.dial :air2 @21:50:18.81 AS.reconfigure ASslow1 @21:50:18.84 AS.seek 0.009ml @21:50:21.16 AS.reconfigure ASslow0 @21:50:21.20 AS.seek 1.000ml @21:52:02.53 ATV.dial AIV @21:52:03.08 AS.seek 0.020ml @21:53:43.36 ATV.dial :waste @21:53:44.65 AS.seek 0.000ml @21:53:46.97 AWV.select :air @21:53:47.12 Cleaning PCR @21:53:47.13 AFV.select :carrierSolution @21:53:47.22 delay 2 seconds @21:53:49.23 AS.reconfigure ASconfig @21:53:49.26 AS.seek 0.500ml @21:54:18.70 delay 1 second @21:54:19.71 AFV.select :holdingCoil @21:54:19.80 ASV.dial :waste1 @21:54:20.12 ATV.dial :air3 @21:54:20.69 AS.reconfigure ASslow1 @21:54:20.72 AS.seek 0.510ml @21:54:23.00 delay 2 seconds @21:54:25.01 ATV.dial :bleach @21:54:25.39 AS.seek 0.810ml @21:55:26.81 delay 3 seconds @21:55:29.82 ATV.dial :air3 @21:55:30.17 delay 3 seconds @21:55:33.23 AS.seek 0.835ml @21:55:38.56 delay 3 seconds @21:55:41.57 ATV.dial :bleach @21:55:41.97 AS.seek 0.820ml @21:55:45.25 delay 3 seconds @21:55:48.26 ARV.dial :waste @21:55:48.76 delay 3 seconds @21:55:51.77 ATV.dial :ARV @21:55:52.59 AS.reconfigure ASconfig @21:55:52.62 AS.seek 0.470ml @21:56:13.29 AFV.select :carrierSolution @21:56:13.38 delay 2 seconds @21:56:15.39 AS.seek 1.000ml @21:56:46.59 delay 1 second @21:56:47.60 AFV.select :holdingCoil @21:56:47.69 delay 1 second @21:56:48.70 AS.reconfigure ASfast @21:56:48.73 AS.seek 0.000ml @21:57:16.47 AFV.select :carrierSolution @21:57:16.57 delay 2 seconds @21:57:19.16 AS.reconfigure ASconfig @21:57:19.19 AS.seek 1.000ml @21:58:17.74 delay 1 second @21:58:18.75 AFV.select :holdingCoil @21:58:18.84 delay 1 second @21:58:19.85 AS.reconfigure ASfast @21:58:19.88 AS.seek 0.000ml @21:58:47.63 AFV.select :carrierSolution @21:58:47.74 delay 2 seconds @21:58:49.75 AS.reconfigure ASconfig @21:58:49.78 AS.seek 1.000ml @21:59:48.34 delay 1 second @21:59:49.35 AFV.select :holdingCoil @21:59:49.45 delay 1 second @21:59:50.46 AS.reconfigure ASfast @21:59:50.49 AS.seek 0.000ml @22:00:18.23 ARV.dial between carrier and air1 @22:00:18.75 ATV.dial :air3 @22:00:19.70 AS.reconfigure ASslow1 @22:00:19.73 AS.seek 0.010ml @22:00:22.09 delay 2 seconds @22:00:24.10 ATV.dial :bleach @22:00:24.47 AS.seek 0.810ml @22:03:07.91 delay 3 seconds @22:03:10.92 ATV.dial :air3 @22:03:11.24 delay 3 seconds @22:03:14.30 AS.seek 0.835ml @22:03:19.65 delay 3 seconds @22:03:22.66 ATV.dial :bleach @22:03:23.04 AS.seek 0.820ml @22:03:26.38 delay 3 seconds @22:03:29.39 ATV.dial PCR @22:03:29.72 AS.reconfigure ASconfig @22:03:29.75 AS.seek empty @22:04:17.84 PCR.program :bleachProfile @22:04:17.89 PCR program should complete in < 2:45 @22:04:17.90 PCR.run 10:00 @22:07:25.15 PCR.off @22:07:25.18 AFV.select :carrierSolution @22:07:25.27 delay 2 seconds @22:07:27.28 AS.seek 1.000ml @22:08:25.95 delay 1 second @22:08:26.96 AFV.select :holdingCoil @22:08:27.05 delay 1 second @22:08:28.06 AS.reconfigure ASfast @22:08:28.09 AS.seek 0.000ml @22:08:55.83 delay 3 seconds @22:08:58.84 AFV.select :carrierSolution @22:08:58.93 delay 2 seconds @22:09:00.94 AS.reconfigure ASconfig @22:09:00.97 AS.seek 1.000ml @22:09:59.60 delay 1 second @22:10:00.61 AFV.select :holdingCoil @22:10:00.70 delay 1 second @22:10:01.71 AS.reconfigure ASfast @22:10:01.74 AS.seek 0.000ml @22:10:29.48 delay 3 seconds @22:10:32.49 AFV.select :carrierSolution @22:10:32.58 delay 2 seconds @22:10:34.59 AS.reconfigure ASconfig @22:10:34.62 AS.seek 1.000ml @22:11:33.21 delay 1 second @22:11:34.22 AFV.select :holdingCoil @22:11:34.31 delay 1 second @22:11:35.32 AS.reconfigure ASfast @22:11:35.35 AS.seek 0.000ml @22:12:03.05 delay 3 seconds @22:12:06.06 AFV.select :carrierSolution @22:12:06.15 delay 2 seconds @22:12:08.16 AS.reconfigure ASconfig @22:12:08.19 AS.seek 1.000ml @22:13:06.80 delay 1 second @22:13:07.81 AFV.select :holdingCoil @22:13:07.90 delay 1 second @22:13:08.91 AS.reconfigure ASfast @22:13:08.94 AS.seek 0.000ml @22:13:36.66 delay 3 seconds @22:13:39.67 AFV.select :carrierSolution @22:13:39.76 delay 2 seconds @22:13:41.77 AS.reconfigure ASconfig @22:13:41.81 AS.seek 1.000ml @22:14:40.39 delay 1 second @22:14:41.40 AFV.select :holdingCoil @22:14:41.49 delay 1 second @22:14:42.50 AS.reconfigure ASnorm @22:14:42.53 AS.seek 0.000ml @22:15:33.97 AFV.select :carrierSolution @22:15:34.06 delay 2 seconds @22:15:36.07 AS.reconfigure ASconfig @22:15:36.10 AS.seek 1.000ml @22:16:34.68 delay 1 second @22:16:35.69 AFV.select :holdingCoil @22:16:35.78 delay 1 second @22:16:36.79 ATV.dial :waste @22:16:37.69 AS.reconfigure ASnorm @22:16:37.72 AS.seek 0.500ml @22:17:03.66 ATV.dial :waste2 @22:17:04.77 AS.seek 0.000ml @22:17:30.66 AS.stop @22:17:30.68 CC.stop @22:17:30.71 CS.stop @22:17:30.73 PC.stop @22:17:30.76 PS.stop @22:17:30.79 Forearm.stop @22:17:30.81 Elbow.stop @22:17:30.84 SS.stop @22:17:30.91 Sleepy.power :raw,:OFF @22:17:30.92 Sleepy.power :dwarf,:OFF Shutdown @22:17:30.93 GoodNews.email "MFBPCR completed",Subject => "Shutdown" @22:32:40.45 Session Begun @22:32:53.44 -> showlog @22:40:37.79 -> abort! "Neo is running hydrophobic filter, need to recover and reload" @22:40:37.81 CmdServer::Client::Error in kevan -- Aborting Mission: Neo is running hydrophobic filter, need to recover and reload @22:40:37.83 BadNews.email "Neo is running hydrophobic filter, need to recover and reload",Subject => "14feb14sccwrp ABORTED!" @22:40:57.93 Session Ended hostname: ESPneo: Host name lookup failure @13:34:46.02PST26-Feb-14 ESP2 v3.20 I2C Gateway v3.01 @13:34:46.35 Powering up Dwarves @13:34:52.66
Configuration for neo by maro@mbari.org /home/esp/esp2/shallow/neo/configure.rb @13:34:52.75 Configuration for MFB10 by preston@mbari.org /home/esp/esp2/mfb/MFB10/configure.rb @13:34:55.92 Sleepy.power :raw,:ON @13:34:56.61 SPE.coast @13:34:56.65 SPE.configure SPEconfig @13:34:56.68 AS.stop @13:34:56.71 AS.configure ASconfig @13:34:56.74 CC.stop @13:34:56.76 CC.configure CCconfig @13:34:56.79 CS.stop @13:34:56.81 CS.configure CSconfig @13:34:56.84 PC.stop @13:34:56.86 PC.configure PCconfig @13:34:56.89 PS.stop @13:34:56.91 PS.configure PSconfig @13:34:56.97 Forearm.stop @13:34:56.99 Forearm.configure LooseForearm @13:34:57.02 Elbow.stop @13:34:57.04 Elbow.configure LooseElbow @13:34:57.10 SC.stop @13:34:57.12 SC.configure SCloose @13:34:57.15 SE.stop @13:34:57.17 SE.configure SEconfig @13:34:57.20 SS.stop @13:34:57.22 SS.configure SSconfig @13:34:57.25 CH.coast @13:34:57.29 CH.configure CHconfig @13:34:57.32 PH.coast @13:34:57.36 PH.configure PHconfig @13:34:57.42 PCR.off @13:34:57.45 PCR.pidtemp= 50,0.1,0,300,25,25 @13:34:57.48 PCR.caltemp= 6.6661,0.0344,0.0 @13:34:57.51 All dwarves are running firmware version 3.74 @13:34:58.05 GoodNews.email "Starting w/pucks in tube 2\n ",Subject => "14feb25sccwrp begun" @13:34:58.12 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @13:34:58.15
Asleep til +0:10 @13:34:58.17 Retry #1 of 10 after 5:00 @13:34:58.18
Sleeping until 13:35 @13:34:58.21 Sleepy.power :raw,:OFF @13:34:58.22 Sleepy.power :dwarf,:OFF Shutdown @13:35:07.85
Powering up Dwarves /home/esp/esp2/lib/log.rb:166:in `initialize': Another process is already writing to /var/log/esp/real.log (Log::Locked) from /home/esp/esp2/lib/log.rb:553:in `initialize' from /home/esp/esp2/mode/real.rb:12:in `new' from /home/esp/esp2/mode/real.rb:12 from /home/esp/esp2/lib/framework.rb:26:in `load' from /home/esp/esp2/lib/framework.rb:26:in `ld' from /home/esp/esp2/lib/framework.rb:44:in `ldMode' from /home/esp/esp2/lib/esp.rb:317 @13:35:13.00 Sleepy.power :raw,:ON @13:35:13.46 SPE.configure SPEconfig @13:35:13.48 AS.configure ASconfig @13:35:13.51 CC.configure CCconfig @13:35:13.54 CS.configure CSconfig @13:35:13.57 PC.configure PCconfig @13:35:13.60 PS.configure PSconfig @13:35:13.63 Forearm.configure LooseForearm @13:35:13.66 Elbow.configure LooseElbow @13:35:13.69 SC.configure SCloose @13:35:13.72 SE.configure SEconfig @13:35:13.75 SS.configure SSconfig @13:35:13.78 CH.configure CHconfig @13:35:13.80 PH.configure PHconfig @13:35:13.83 Valve.reset @13:35:14.50 PCR.off @13:35:14.52 PCR.pidtemp= 50,0.1,0,300,25,25 @13:35:14.55 PCR.caltemp= 6.6661,0.0344,0.0 @13:35:14.67 Intake.select :close @13:35:14.68 Exhaust.select :close CTV.dial :air @13:35:14.72 PSV.dial :air @13:35:14.76
PC.reconfigure PCconfig @13:35:14.81 PC.home.seek open @13:35:14.85 CSV.dial :waste1 @13:35:14.90 PS.reconfigure PSconfig @13:35:14.95 SSV.dial :spit,avoiding => exhaust @13:35:14.97 PS.home.seek 0.000ml @13:35:15.12 SS.reconfigure SSconfig @13:35:15.29 CS.reconfigure CSfast @13:35:15.37 SS.home.seek 0.00ml @13:35:15.39 CS.home.seek 0.000ml @13:35:15.46 CC.reconfigure CCconfig @13:35:15.49 CC.home.seek open @13:35:25.24 PS.coast @13:35:25.32
CC.seek open @13:35:25.35 PC.seek open @13:35:25.48
Forearm.reconfigure LooseForearm @13:35:25.51 Forearm.home.seek retract @13:35:26.24 Hand.grip @13:35:26.26
Elbow.jog -1500 @13:35:26.77 Elbow.reconfigure LooseElbow @13:35:26.81 Elbow.home.seek clear @13:35:30.25 SE.reconfigure SEconfig @13:35:30.28 SE.home.seek down @13:35:30.82 SC.reconfigure SCsloppy @13:35:30.86 SC.home.seek initial @13:35:41.29 Forearm.seek retract @13:35:41.34 Elbow.seek hammer @13:35:42.54 Forearm.reconfigure TightForearm @13:35:42.59 Forearm.seek 2 @13:35:43.59 Forearm.seek retract @13:35:44.70 Forearm.reconfigure LooseForearm @13:35:45.07PST26-Feb-14 ===> STARTED wqPCR (phase #1) <=== @13:35:45.15 Starting mfb @13:35:45.16 pcrBAC sampling at most 1000ml, wcr at most 1000ml @13:35:45.18 Loading sh1 puck from tube 2 into Collection Clamp @13:35:45.27 Readying the MFB @13:35:45.28 Homing MFB Syringe @13:35:45.29 AFV.select :holdingCoil @13:35:45.33
Hand.release @13:35:45.42 ATV.dial :air2 @13:35:46.27
SC.reconfigure SCloose @13:35:46.35 AS.reconfigure ASconfig @13:35:46.42 AS.home.seek home @13:35:46.51
Elbow.reconfigure TightElbow @13:35:46.54 Elbow.seek 2 @13:35:46.68 Forearm.reconfigure TightForearm @13:35:46.72 Forearm.seek 2 + 800 counts @13:35:47.73 SE.reconfigure SEpick @13:35:47.78 SE.seek beamBreak @13:35:50.50 SE.configure SEconfig @13:35:50.62 Elbow.seek 2 @13:35:50.78 Forearm.seek 2 + 300 counts @13:35:51.02 Forearm.reconfigure LooseForearm @13:35:51.06 Hand.grip @13:35:51.74 SE.seek down @13:35:51.88
Forearm.seek retract @13:35:52.84 Elbow.seek CC @13:35:54.36 Elbow.reconfigure LooseElbow @13:35:54.40 SE.coast @13:35:54.50 AFV.select :carrierSolution @13:35:54.51
Forearm.reconfigure TightForearm @13:35:54.57 Forearm.seek CC @13:35:54.62 delay 2 seconds @13:35:55.67
CC.seek closed @13:35:56.63 AS.seek 1.000ml @13:35:59.42
Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.99 @13:35:59.43 Hand.release @13:36:00.15 Forearm.reconfigure LooseForearm @13:36:00.18 Forearm.seek retract @13:36:01.16 SC.coast @13:36:01.19 collectSample 1000ml w/bubble point of 55psi @13:36:01.20 Priming 7ml Intake.select :close @13:36:01.21 Exhaust.select :close @13:36:01.22 SS.hold @13:36:01.38 Calibrating pressure and temperature sensors @13:36:01.40 CTV.dial :air @13:36:01.41 SSV.dial :spit,avoiding => exhaust @13:36:01.42 SS.reconfigure SSfast @13:36:01.45 SS.seek 5.00ml @13:36:04.40 SSV.dial CBV @13:36:04.42
CBV.dial SSV @13:36:05.75 delay 3.8 seconds @13:36:09.64 SSV.dial :spit @13:36:10.92 SS.seek 0.00ml @13:36:13.60 SSV.dial CBV @13:36:14.90 CTV.dial :intake @13:36:15.61 SSV.dial CBV @13:36:15.62
CBV.dial SSV @13:36:15.63 Allowing 55s for 7.0ml of remaining 7.0ml @13:36:15.64 Intake.select :open @13:36:17.77 Intake @33.0psia, vacuum from 20.0 to 24.0psi @13:36:17.78 SS.reconfigure forSampling @13:36:17.82 SS.seek 7.00ml @13:36:23.05 Stroke completed -- waiting for pressure to equalize ... @13:36:23.69 Intake.select :close @13:36:23.83 SSV.dial :exhaust @13:36:24.57 Exhaust.select :open @13:36:24.66 SS.reconfigure SSexhale @13:36:24.70 SS.seek 0.00ml @13:36:28.19 Exhaust.select :close @13:36:28.28 Sampling 1000.0ml @13:36:28.29 Intake.select :close @13:36:28.30 Exhaust.select :close @13:36:28.31 SS.reconfigure SSconfig @13:36:28.34 SS.hold @13:36:28.49 CTV.dial :intake @13:36:28.50 SSV.dial CBV @13:36:28.52
CBV.dial SSV @13:36:29.28 Allowing 185s for 25.0ml of remaining 1000.0ml Intake.select :open @13:36:31.41 Intake @32.9psia, vacuum from 20.0 to 24.0psi @13:36:31.42 SS.reconfigure forSampling @13:36:31.46 SS.seek 25.00ml @13:36:45.60 Stroke completed -- waiting for pressure to equalize ... @13:36:46.24 Intake.select :close @13:36:46.37 SSV.dial :exhaust @13:36:47.12 Exhaust.select :open @13:36:47.21 SS.reconfigure SSexhale @13:36:47.25 SS.seek 0.00ml @13:36:48.41 delay 1 second @13:36:49.42 AFV.select :holdingCoil @13:36:49.51 delay 1 second @13:36:50.52 ATV.dial :waste @13:36:51.43 AS.reconfigure ASnorm @13:36:51.46 AS.home.seek empty @13:36:56.02
Exhaust.select :close @13:36:56.12 SSV.dial CBV @13:36:56.14
CBV.dial SSV @13:36:56.90 Allowing 185s for 25.0ml of remaining 975.0ml @13:36:56.91 Intake.select :open @13:36:59.04 Intake @33.3psia, vacuum from 20.0 to 24.0psi @13:36:59.05 SS.reconfigure forSampling @13:36:59.09 SS.seek 25.00ml @13:37:13.80 Stroke completed -- waiting for pressure to equalize ... @13:37:14.44 Intake.select :close @13:37:14.57 SSV.dial :exhaust @13:37:15.32 Exhaust.select :open @13:37:15.43 SS.reconfigure SSexhale @13:37:15.47 SS.seek 0.00ml @13:37:24.21 Exhaust.select :close @13:37:24.30 SSV.dial CBV @13:37:24.32
CBV.dial SSV @13:37:25.08 Allowing 185s for 25.0ml of remaining 950.0ml @13:37:25.09 Intake.select :open @13:37:27.22 Intake @33.3psia, vacuum from 20.0 to 24.0psi @13:37:27.23 SS.reconfigure forSampling @13:37:27.27 SS.seek 25.00ml @13:37:41.06 Stroke completed -- waiting for pressure to equalize ... @13:37:41.70 Intake.select :close @13:37:41.83 SSV.dial :exhaust @13:37:42.57 Exhaust.select :open @13:37:42.67 SS.reconfigure SSexhale @13:37:42.71 SS.seek 0.00ml @13:37:44.75 ATV.dial :waste @13:37:44.76 ARV.dial between carrier and air1 @13:37:44.82 ASV.dial :waste1 @13:37:51.31
Exhaust.select :close @13:37:51.40 SSV.dial CBV @13:37:51.42
CBV.dial SSV @13:37:52.17 Allowing 185s for 25.0ml of remaining 925.0ml @13:37:52.18 Intake.select :open @13:37:54.31 Intake @33.2psia, vacuum from 20.0 to 24.0psi @13:37:54.32 SS.reconfigure forSampling @13:37:54.36 SS.seek 25.00ml @13:38:08.45 Stroke completed -- waiting for pressure to equalize ... @13:38:09.66 Intake.select :close @13:38:09.80 SSV.dial :exhaust @13:38:10.53 Exhaust.select :open @13:38:10.64 SS.reconfigure SSexhale @13:38:10.68 SS.seek 0.00ml @13:38:19.21 Exhaust.select :close @13:38:19.30 SSV.dial CBV @13:38:19.32
CBV.dial SSV @13:38:20.07 Allowing 185s for 25.0ml of remaining 900.0ml @13:38:20.08 Intake.select :open @13:38:22.21 Intake @33.2psia, vacuum from 20.0 to 24.0psi @13:38:22.22 SS.reconfigure forSampling @13:38:22.26 SS.seek 25.00ml @13:38:36.34 Stroke completed -- waiting for pressure to equalize ... @13:38:36.98 Intake.select :close @13:38:37.12 SSV.dial :exhaust @13:38:37.85 Exhaust.select :open @13:38:37.95 SS.reconfigure SSexhale @13:38:37.99 SS.seek 0.00ml @13:38:46.66 Exhaust.select :close @13:38:46.75 SSV.dial CBV @13:38:46.77
CBV.dial SSV @13:38:47.52 Allowing 185s for 25.0ml of remaining 875.0ml @13:38:47.53 Intake.select :open @13:38:49.66 Intake @33.3psia, vacuum from 20.0 to 24.0psi @13:38:49.67 SS.reconfigure forSampling @13:38:49.71 SS.seek 25.00ml @13:39:03.79 Stroke completed -- waiting for pressure to equalize ... @13:39:04.44 Intake.select :close @13:39:04.57 SSV.dial :exhaust @13:39:05.30 Exhaust.select :open @13:39:05.40 SS.reconfigure SSexhale @13:39:05.44 SS.seek 0.00ml @13:39:15.35 Exhaust.select :close @13:39:15.44 SSV.dial CBV @13:39:15.46
CBV.dial SSV @13:39:16.21 Allowing 185s for 25.0ml of remaining 850.0ml @13:39:16.22 Intake.select :open @13:39:18.35 Intake @33.2psia, vacuum from 20.0 to 24.0psi @13:39:18.36 SS.reconfigure forSampling @13:39:18.40 SS.seek 25.00ml @13:39:29.06 Session Begun @13:39:29.09 -> Sleepy.cycleModemPower! 9 @13:39:29.11 Session Ended @13:39:32.55
Stroke completed -- waiting for pressure to equalize ... @13:39:33.28 Intake.select :close @13:39:33.52 SSV.dial :exhaust @13:39:34.26 Exhaust.select :open @13:39:34.36 SS.reconfigure SSexhale @13:39:34.40 SS.seek 0.00ml @13:39:43.13 Exhaust.select :close @13:39:43.22 SSV.dial CBV @13:39:43.24
CBV.dial SSV @13:39:44.01 Allowing 185s for 25.0ml of remaining 825.0ml @13:39:44.02 Intake.select :open @13:39:46.15 Intake @33.2psia, vacuum from 20.0 to 24.0psi @13:39:46.16 SS.reconfigure forSampling @13:39:46.20 SS.seek 25.00ml @13:39:58.19 GoodNews.email "Starting w/pucks in tube 2\n ",Subject => "14feb25sccwrp begun" @13:39:58.21 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @13:39:58.22 Retry #2 of 10 after 5:00 @13:40:00.40
Stroke completed -- waiting for pressure to equalize ... @13:40:01.04 Intake.select :close @13:40:01.17 SSV.dial :exhaust @13:40:01.93 Exhaust.select :open @13:40:02.02 SS.reconfigure SSexhale @13:40:02.06 SS.seek 0.00ml @13:40:10.86 Exhaust.select :close @13:40:10.95 SSV.dial CBV @13:40:10.97
CBV.dial SSV @13:40:11.74 Allowing 185s for 25.0ml of remaining 800.0ml @13:40:11.75 Intake.select :open @13:40:13.88 Intake @33.3psia, vacuum from 20.0 to 24.0psi @13:40:13.89 SS.reconfigure forSampling @13:40:13.93 SS.seek 25.00ml @13:40:27.71 Stroke completed -- waiting for pressure to equalize ... @13:40:28.35 Intake.select :close @13:40:28.48 SSV.dial :exhaust @13:40:29.24 Exhaust.select :open @13:40:29.34 SS.reconfigure SSexhale @13:40:29.38 SS.seek 0.00ml @13:40:38.16 Exhaust.select :close @13:40:38.25 SSV.dial CBV @13:40:38.27
CBV.dial SSV @13:40:39.03 Allowing 185s for 25.0ml of remaining 775.0ml @13:40:39.04 Intake.select :open @13:40:41.17 Intake @33.2psia, vacuum from 20.0 to 24.0psi @13:40:41.18 SS.reconfigure forSampling @13:40:41.22 SS.seek 25.00ml @13:40:55.41 Stroke completed -- waiting for pressure to equalize ... @13:40:56.05 Intake.select :close @13:40:56.18 SSV.dial :exhaust @13:40:56.94 Exhaust.select :open @13:40:57.03 SS.reconfigure SSexhale @13:40:57.07 SS.seek 0.00ml @13:41:05.50 Exhaust.select :close @13:41:05.59 SSV.dial CBV @13:41:05.61
CBV.dial SSV @13:41:06.38 Allowing 185s for 25.0ml of remaining 750.0ml @13:41:06.39 Intake.select :open @13:41:08.52 Intake @33.2psia, vacuum from 20.0 to 24.0psi @13:41:08.53 SS.reconfigure forSampling @13:41:08.57 SS.seek 25.00ml @13:41:22.73 Stroke completed -- waiting for pressure to equalize ... @13:41:23.37 Intake.select :close @13:41:23.50 SSV.dial :exhaust @13:41:24.25 Exhaust.select :open @13:41:24.34 SS.reconfigure SSexhale @13:41:24.38 SS.seek 0.00ml @13:41:33.36 Exhaust.select :close @13:41:33.45 SSV.dial CBV @13:41:33.47
CBV.dial SSV @13:41:34.22 Allowing 185s for 25.0ml of remaining 725.0ml @13:41:34.23 Intake.select :open @13:41:36.36 Intake @33.0psia, vacuum from 20.0 to 24.0psi @13:41:36.37 SS.reconfigure forSampling @13:41:36.41 SS.seek 25.00ml @13:41:50.39 Stroke completed -- waiting for pressure to equalize ... @13:41:51.03 Intake.select :close @13:41:51.16 SSV.dial :exhaust @13:41:51.92 Exhaust.select :open @13:41:52.02 SS.reconfigure SSexhale @13:41:52.06 SS.seek 0.00ml @13:42:00.64 Exhaust.select :close @13:42:00.73 SSV.dial CBV @13:42:00.75
CBV.dial SSV @13:42:01.51 Allowing 185s for 25.0ml of remaining 700.0ml @13:42:01.52 Intake.select :open @13:42:03.65 Intake @33.2psia, vacuum from 20.0 to 24.0psi @13:42:03.66 SS.reconfigure forSampling @13:42:03.70 SS.seek 25.00ml @13:42:17.78 Stroke completed -- waiting for pressure to equalize ... @13:42:18.42 Intake.select :close @13:42:18.55 SSV.dial :exhaust @13:42:19.29 Exhaust.select :open @13:42:19.38 SS.reconfigure SSexhale @13:42:19.42 SS.seek 0.00ml @13:42:28.09 Exhaust.select :close @13:42:28.18 SSV.dial CBV @13:42:28.20
CBV.dial SSV @13:42:28.96 Allowing 185s for 25.0ml of remaining 675.0ml @13:42:28.97 Intake.select :open @13:42:31.11 Intake @33.1psia, vacuum from 20.0 to 24.0psi @13:42:31.12 SS.reconfigure forSampling @13:42:31.16 SS.seek 25.00ml @13:42:44.93 Stroke completed -- waiting for pressure to equalize ... @13:42:45.57 Intake.select :close @13:42:45.70 SSV.dial :exhaust @13:42:46.43 Exhaust.select :open @13:42:46.52 SS.reconfigure SSexhale @13:42:46.56 SS.seek 0.00ml @13:42:55.06 Exhaust.select :close @13:42:55.15 SSV.dial CBV @13:42:55.17
CBV.dial SSV @13:42:55.92 Allowing 185s for 25.0ml of remaining 650.0ml @13:42:55.93 Intake.select :open @13:42:58.06 Intake @33.1psia, vacuum from 20.0 to 24.0psi @13:42:58.08 SS.reconfigure forSampling @13:42:58.11 SS.seek 25.00ml @13:43:12.40 Stroke completed -- waiting for pressure to equalize ... @13:43:13.04 Intake.select :close @13:43:13.17 SSV.dial :exhaust @13:43:13.90 Exhaust.select :open @13:43:14.00 SS.reconfigure SSexhale @13:43:14.04 SS.seek 0.00ml @13:43:22.56 Exhaust.select :close @13:43:23.26 SSV.dial CBV @13:43:23.28
CBV.dial SSV @13:43:24.02 Allowing 185s for 25.0ml of remaining 625.0ml @13:43:24.03 Intake.select :open @13:43:26.16 Intake @33.2psia, vacuum from 20.0 to 24.0psi @13:43:26.17 SS.reconfigure forSampling @13:43:26.21 SS.seek 25.00ml @13:43:40.40 Stroke completed -- waiting for pressure to equalize ... @13:43:41.04 Intake.select :close @13:43:41.19 SSV.dial :exhaust @13:43:41.91 Exhaust.select :open @13:43:42.00 SS.reconfigure SSexhale @13:43:42.04 SS.seek 0.00ml @13:43:50.65 Exhaust.select :close @13:43:50.74 SSV.dial CBV @13:43:50.76
CBV.dial SSV @13:43:51.51 Allowing 185s for 25.0ml of remaining 600.0ml @13:43:51.52 Intake.select :open @13:43:53.65 Intake @33.2psia, vacuum from 20.0 to 24.0psi @13:43:53.66 SS.reconfigure forSampling @13:43:53.70 SS.seek 25.00ml @13:44:07.87 Stroke completed -- waiting for pressure to equalize ... @13:44:08.52 Intake.select :close @13:44:08.65 SSV.dial :exhaust @13:44:09.38 Exhaust.select :open @13:44:09.47 SS.reconfigure SSexhale @13:44:09.51 SS.seek 0.00ml @13:44:18.21 Exhaust.select :close @13:44:18.30 SSV.dial CBV @13:44:18.32
CBV.dial SSV @13:44:19.07 Allowing 185s for 25.0ml of remaining 575.0ml @13:44:19.08 Intake.select :open @13:44:21.21 Intake @33.1psia, vacuum from 20.0 to 24.0psi @13:44:21.22 SS.reconfigure forSampling @13:44:21.26 SS.seek 25.00ml @13:44:34.17 Session Begun @13:44:35.01
Stroke completed -- waiting for pressure to equalize ... @13:44:35.65 Intake.select :close @13:44:35.81 SSV.dial :exhaust @13:44:36.55 Exhaust.select :open @13:44:36.66 SS.reconfigure SSexhale @13:44:36.71 SS.seek 0.00ml @13:44:40.92 -> progress @13:44:45.50
Exhaust.select :close @13:44:45.61 SSV.dial CBV @13:44:45.63
CBV.dial SSV @13:44:46.40 Allowing 185s for 25.0ml of remaining 550.0ml @13:44:46.41 Intake.select :open @13:44:48.57 Intake @33.1psia, vacuum from 20.0 to 24.0psi @13:44:48.58 SS.reconfigure forSampling @13:44:48.64 SS.seek 25.00ml @13:44:58.22 GoodNews.email "Starting w/pucks in tube 2\n ",Subject => "14feb25sccwrp begun" @13:45:02.44
Stroke completed -- waiting for pressure to equalize ... @13:45:03.09 Intake.select :close @13:45:03.24 SSV.dial :exhaust @13:45:04.00 Exhaust.select :open @13:45:04.09 SS.reconfigure SSexhale @13:45:04.14 SS.seek 0.00ml @13:45:12.41 GoodNews.email "Then, in tube 2: wqPCR\n/home/esp/esp2/mission/14feb25sccwrp.rb:48",Subject => "Asleep until 13:35" @13:45:13.95
Exhaust.select :close @13:45:14.08 SSV.dial CBV @13:45:14.10
CBV.dial SSV @13:45:14.86 Allowing 185s for 25.0ml of remaining 525.0ml @13:45:14.88 Intake.select :open @13:45:17.04 Intake @33.1psia, vacuum from 20.0 to 24.0psi @13:45:17.05 SS.reconfigure forSampling @13:45:17.11 SS.seek 25.00ml @13:45:23.06 -> exit @13:45:23.07 Session Ended @13:45:25.92 BACfans.email "Can@13:45:00, 23.8C, 44% humidity, 14.4psia, 13.525V, 1.672A, 1.672A avg, 22.61W\nCTD data unavailable\nISUS data unavailable",Subject => "pcrBAC sampling at most 1000ml, wcr at most 1000ml" @13:45:31.22
Stroke completed -- waiting for pressure to equalize ... @13:45:31.86 Intake.select :close @13:45:31.99 SSV.dial :exhaust @13:45:32.72 Exhaust.select :open @13:45:32.82 SS.reconfigure SSexhale @13:45:32.86 SS.seek 0.00ml @13:45:41.63 Exhaust.select :close @13:45:41.72 SSV.dial CBV @13:45:41.74
CBV.dial SSV @13:45:42.50 Allowing 185s for 25.0ml of remaining 500.0ml Intake.select :open @13:45:44.63 Intake @33.1psia, vacuum from 20.0 to 24.0psi @13:45:44.64 SS.reconfigure forSampling @13:45:44.68 SS.seek 25.00ml @13:45:58.83 Stroke completed -- waiting for pressure to equalize ... @13:45:59.47 Intake.select :close @13:45:59.60 SSV.dial :exhaust @13:46:00.34 Exhaust.select :open @13:46:00.43 SS.reconfigure SSexhale @13:46:00.47 SS.seek 0.00ml @13:46:09.11 Exhaust.select :close @13:46:09.20 SSV.dial CBV @13:46:09.22
CBV.dial SSV @13:46:09.97 Allowing 185s for 25.0ml of remaining 475.0ml @13:46:09.98 Intake.select :open @13:46:12.11 Intake @33.0psia, vacuum from 20.0 to 24.0psi @13:46:12.12 SS.reconfigure forSampling @13:46:12.16 SS.seek 25.00ml @13:46:26.72 Stroke completed -- waiting for pressure to equalize ... @13:46:27.36 Intake.select :close @13:46:27.49 SSV.dial :exhaust @13:46:28.23 Exhaust.select :open @13:46:28.32 SS.reconfigure SSexhale @13:46:28.36 SS.seek 0.00ml @13:46:37.01 Exhaust.select :close @13:46:37.10 SSV.dial CBV @13:46:37.12
CBV.dial SSV @13:46:37.87 Allowing 185s for 25.0ml of remaining 450.0ml @13:46:37.88 Intake.select :open @13:46:40.01 Intake @33.0psia, vacuum from 20.0 to 24.0psi @13:46:40.02 SS.reconfigure forSampling @13:46:40.06 SS.seek 25.00ml @13:46:54.29 Stroke completed -- waiting for pressure to equalize ... @13:46:54.93 Intake.select :close @13:46:55.06 SSV.dial :exhaust @13:46:55.79 Exhaust.select :open @13:46:55.88 SS.reconfigure SSexhale @13:46:55.92 SS.seek 0.00ml @13:47:04.56 Exhaust.select :close @13:47:04.65 SSV.dial CBV @13:47:04.67
CBV.dial SSV @13:47:05.42 Allowing 185s for 25.0ml of remaining 425.0ml @13:47:05.43 Intake.select :open @13:47:07.56 Intake @33.1psia, vacuum from 20.0 to 24.0psi @13:47:07.57 SS.reconfigure forSampling @13:47:07.61 SS.seek 25.00ml @13:47:21.78 Stroke completed -- waiting for pressure to equalize ... @13:47:22.42 Intake.select :close @13:47:22.55 SSV.dial :exhaust @13:47:23.29 Exhaust.select :open @13:47:23.38 SS.reconfigure SSexhale @13:47:23.42 SS.seek 0.00ml @13:47:32.07 Exhaust.select :close @13:47:32.16 SSV.dial CBV @13:47:32.18
CBV.dial SSV @13:47:32.93 Allowing 185s for 25.0ml of remaining 400.0ml @13:47:32.94 Intake.select :open @13:47:35.07 Intake @33.1psia, vacuum from 20.0 to 24.0psi @13:47:35.08 SS.reconfigure forSampling @13:47:35.12 SS.seek 25.00ml @13:47:49.34 Stroke completed -- waiting for pressure to equalize ... @13:47:49.98 Intake.select :close @13:47:50.11 SSV.dial :exhaust @13:47:50.85 Exhaust.select :open @13:47:50.94 SS.reconfigure SSexhale @13:47:50.98 SS.seek 0.00ml @13:47:59.73 Exhaust.select :close @13:47:59.82 SSV.dial CBV @13:47:59.84
CBV.dial SSV @13:48:00.59 Allowing 185s for 25.0ml of remaining 375.0ml @13:48:00.60 Intake.select :open @13:48:02.73 Intake @33.0psia, vacuum from 20.0 to 24.0psi @13:48:02.74 SS.reconfigure forSampling @13:48:02.78 SS.seek 25.00ml @13:48:17.02 Stroke completed -- waiting for pressure to equalize ... @13:48:17.66 Intake.select :close @13:48:17.79 SSV.dial :exhaust @13:48:18.52 Exhaust.select :open @13:48:18.61 SS.reconfigure SSexhale @13:48:18.65 SS.seek 0.00ml @13:48:27.10 Exhaust.select :close @13:48:27.19 SSV.dial CBV @13:48:27.21
CBV.dial SSV @13:48:27.96 Allowing 185s for 25.0ml of remaining 350.0ml @13:48:27.97 Intake.select :open @13:48:30.10 Intake @32.9psia, vacuum from 20.0 to 24.0psi @13:48:30.11 SS.reconfigure forSampling @13:48:30.15 SS.seek 25.00ml @13:48:43.94 Stroke completed -- waiting for pressure to equalize ... @13:48:44.57 Intake.select :close @13:48:44.71 SSV.dial :exhaust @13:48:45.44 Exhaust.select :open @13:48:45.53 SS.reconfigure SSexhale @13:48:45.57 SS.seek 0.00ml @13:48:54.15 Exhaust.select :close @13:48:54.24 SSV.dial CBV @13:48:54.26
CBV.dial SSV @13:48:55.01 Allowing 185s for 25.0ml of remaining 325.0ml @13:48:55.02 Intake.select :open @13:48:57.15 Intake @32.8psia, vacuum from 20.0 to 24.0psi @13:48:57.16 SS.reconfigure forSampling @13:48:57.20 SS.seek 25.00ml @13:49:11.01 Stroke completed -- waiting for pressure to equalize ... @13:49:11.64 Intake.select :close @13:49:11.78 SSV.dial :exhaust @13:49:12.52 Exhaust.select :open @13:49:12.61 SS.reconfigure SSexhale @13:49:12.65 SS.seek 0.00ml @13:49:21.79 Exhaust.select :close @13:49:21.88 SSV.dial CBV @13:49:21.90
CBV.dial SSV @13:49:22.65 Allowing 185s for 25.0ml of remaining 300.0ml @13:49:22.66 Intake.select :open @13:49:24.79 Intake @32.9psia, vacuum from 20.0 to 24.0psi @13:49:24.80 SS.reconfigure forSampling @13:49:24.84 SS.seek 25.00ml @13:49:39.16 Stroke completed -- waiting for pressure to equalize ... @13:49:40.26 Intake.select :close @13:49:40.39 SSV.dial :exhaust @13:49:41.13 Exhaust.select :open @13:49:41.22 SS.reconfigure SSexhale @13:49:41.26 SS.seek 0.00ml @13:49:50.00 Exhaust.select :close @13:49:50.09 SSV.dial CBV @13:49:50.11
CBV.dial SSV @13:49:50.86 Allowing 185s for 25.0ml of remaining 275.0ml @13:49:50.87 Intake.select :open @13:49:53.00 Intake @33.0psia, vacuum from 20.0 to 24.0psi @13:49:53.01 SS.reconfigure forSampling @13:49:53.05 SS.seek 25.00ml @13:50:07.21 Stroke completed -- waiting for pressure to equalize ... @13:50:07.85 Intake.select :close @13:50:08.00 SSV.dial :exhaust @13:50:08.74 Exhaust.select :open @13:50:08.83 SS.reconfigure SSexhale @13:50:08.87 SS.seek 0.00ml @13:50:17.81 Exhaust.select :close @13:50:17.90 SSV.dial CBV @13:50:17.92
CBV.dial SSV @13:50:18.67 Allowing 185s for 25.0ml of remaining 250.0ml @13:50:18.68 Intake.select :open @13:50:20.81 Intake @32.9psia, vacuum from 20.0 to 24.0psi @13:50:20.82 SS.reconfigure forSampling @13:50:20.86 SS.seek 25.00ml @13:50:34.95 Stroke completed -- waiting for pressure to equalize ... @13:50:35.59 Intake.select :close @13:50:35.72 SSV.dial :exhaust @13:50:36.45 Exhaust.select :open @13:50:36.54 SS.reconfigure SSexhale @13:50:36.58 SS.seek 0.00ml @13:50:45.33 Exhaust.select :close @13:50:45.42 SSV.dial CBV @13:50:45.44
CBV.dial SSV @13:50:46.19 Allowing 185s for 25.0ml of remaining 225.0ml @13:50:46.20 Intake.select :open @13:50:48.33 Intake @32.8psia, vacuum from 20.0 to 24.0psi @13:50:48.34 SS.reconfigure forSampling @13:50:48.38 SS.seek 25.00ml @13:51:02.56 Stroke completed -- waiting for pressure to equalize ... @13:51:03.20 Intake.select :close @13:51:03.33 SSV.dial :exhaust @13:51:04.06 Exhaust.select :open @13:51:04.15 SS.reconfigure SSexhale @13:51:04.19 SS.seek 0.00ml @13:51:12.84 Exhaust.select :close @13:51:12.93 SSV.dial CBV @13:51:12.95
CBV.dial SSV @13:51:13.70 Allowing 185s for 25.0ml of remaining 200.0ml @13:51:13.71 Intake.select :open @13:51:15.84 Intake @32.8psia, vacuum from 20.0 to 24.0psi @13:51:15.85 SS.reconfigure forSampling @13:51:15.89 SS.seek 25.00ml @13:51:29.73 Stroke completed -- waiting for pressure to equalize ... @13:51:30.36 Intake.select :close @13:51:30.50 SSV.dial :exhaust @13:51:31.23 Exhaust.select :open @13:51:31.32 SS.reconfigure SSexhale @13:51:31.36 SS.seek 0.00ml @13:51:40.17 Exhaust.select :close @13:51:40.26 SSV.dial CBV @13:51:40.28
CBV.dial SSV @13:51:41.03 Allowing 185s for 25.0ml of remaining 175.0ml @13:51:41.04 Intake.select :open @13:51:43.17 Intake @32.9psia, vacuum from 20.0 to 24.0psi @13:51:43.18 SS.reconfigure forSampling @13:51:43.22 SS.seek 25.00ml @13:51:57.53 Stroke completed -- waiting for pressure to equalize ... @13:51:58.17 Intake.select :close @13:51:58.30 SSV.dial :exhaust @13:51:59.04 Exhaust.select :open @13:51:59.13 SS.reconfigure SSexhale @13:51:59.17 SS.seek 0.00ml @13:52:07.82 Exhaust.select :close @13:52:07.91 SSV.dial CBV @13:52:07.93
CBV.dial SSV @13:52:08.68 Allowing 185s for 25.0ml of remaining 150.0ml @13:52:08.69 Intake.select :open @13:52:10.82 Intake @32.8psia, vacuum from 20.0 to 24.0psi @13:52:10.83 SS.reconfigure forSampling @13:52:10.87 SS.seek 25.00ml @13:52:25.09 Stroke completed -- waiting for pressure to equalize ... @13:52:25.72 Intake.select :close @13:52:25.86 SSV.dial :exhaust @13:52:26.60 Exhaust.select :open @13:52:26.69 SS.reconfigure SSexhale @13:52:26.73 SS.seek 0.00ml @13:52:35.49 Exhaust.select :close @13:52:35.58 SSV.dial CBV @13:52:35.60
CBV.dial SSV @13:52:36.35 Allowing 185s for 25.0ml of remaining 125.0ml @13:52:36.36 Intake.select :open @13:52:38.49 Intake @32.8psia, vacuum from 20.0 to 24.0psi @13:52:38.50 SS.reconfigure forSampling @13:52:38.54 SS.seek 25.00ml @13:52:52.63 Stroke completed -- waiting for pressure to equalize ... @13:52:53.26 Intake.select :close @13:52:53.40 SSV.dial :exhaust @13:52:54.13 Exhaust.select :open @13:52:54.22 SS.reconfigure SSexhale @13:52:54.26 SS.seek 0.00ml @13:53:02.88 Exhaust.select :close @13:53:02.97 SSV.dial CBV @13:53:02.99
CBV.dial SSV @13:53:03.76 Allowing 185s for 25.0ml of remaining 100.0ml @13:53:03.77 Intake.select :open @13:53:05.90 Intake @32.8psia, vacuum from 20.0 to 24.0psi @13:53:05.91 SS.reconfigure forSampling @13:53:05.95 SS.seek 25.00ml @13:53:20.10 Stroke completed -- waiting for pressure to equalize ... @13:53:20.73 Intake.select :close @13:53:20.87 SSV.dial :exhaust @13:53:21.62 Exhaust.select :open @13:53:21.71 SS.reconfigure SSexhale @13:53:21.75 SS.seek 0.00ml @13:53:30.90 Exhaust.select :close @13:53:30.99 SSV.dial CBV @13:53:31.01
CBV.dial SSV @13:53:31.77 Allowing 185s for 25.0ml of remaining 75.0ml @13:53:31.78 Intake.select :open @13:53:33.91 Intake @32.8psia, vacuum from 20.0 to 24.0psi @13:53:33.92 SS.reconfigure forSampling @13:53:33.96 SS.seek 25.00ml @13:53:48.12 Stroke completed -- waiting for pressure to equalize ... @13:53:48.76 Intake.select :close @13:53:48.90 SSV.dial :exhaust @13:53:49.63 Exhaust.select :open @13:53:49.72 SS.reconfigure SSexhale @13:53:49.76 SS.seek 0.00ml @13:53:58.27 Exhaust.select :close @13:53:58.36 SSV.dial CBV @13:53:58.38
CBV.dial SSV @13:53:59.13 Allowing 185s for 25.0ml of remaining 50.0ml @13:53:59.14 Intake.select :open @13:54:01.27 Intake @32.8psia, vacuum from 20.0 to 24.0psi @13:54:01.28 SS.reconfigure forSampling @13:54:01.32 SS.seek 25.00ml @13:54:15.10 Stroke completed -- waiting for pressure to equalize ... @13:54:15.75 Intake.select :close @13:54:15.91 SSV.dial :exhaust @13:54:16.65 Exhaust.select :open @13:54:16.75 SS.reconfigure SSexhale @13:54:16.80 SS.seek 0.00ml @13:54:25.36 Exhaust.select :close @13:54:25.47 SSV.dial CBV @13:54:25.49
CBV.dial SSV @13:54:26.24 Allowing 185s for 25.0ml of remaining 25.0ml @13:54:26.25 Intake.select :open @13:54:28.40 Intake @32.9psia, vacuum from 20.0 to 24.0psi @13:54:28.41 SS.reconfigure forSampling @13:54:28.44 SS.seek 24.98ml @13:54:42.50 Stroke completed -- waiting for pressure to equalize ... @13:54:43.14 Intake.select :close @13:54:43.27 SSV.dial :exhaust @13:54:44.00 Exhaust.select :open @13:54:44.11 SS.reconfigure SSexhale @13:54:44.15 SS.seek 0.00ml @13:54:52.75 Exhaust.select :close @13:54:52.84 Sampled 1000.0ml @13:54:52.85 collectSample: Complete @13:54:52.86 Evacuating Puck (bubblePt=55.0psi, delay=15.0s) @13:54:52.87 Intake.select :close @13:54:52.92 CSV.dial :waste @13:54:52.94 CS.seek 0.000ml @13:54:53.04 SSV.dial CBV,avoiding => exhaust @13:54:53.07
CBV.dial SSV @13:54:53.11 CSV.dial :air @13:54:53.91 CS.seek 10.000ml @13:54:54.91
CTV.dial :air @13:54:55.67 delay 15 seconds @13:55:10.67 SSV.dial :spit,avoiding => exhaust @13:55:11.97 CTV.dial CSV,avoiding => intake @13:55:11.99
SS.seek 0.00ml @13:55:12.09 SSV.dial CBV,avoiding => exhaust @13:55:17.45 CBV.dial between CSV and waste2b @13:55:18.42 CTV.dial CSV,avoiding => air @13:55:18.43
CSV.dial CTV @13:55:18.86 CS.reconfigure CSpressurize @13:55:18.89 CS.seek 0.000ml @13:55:43.85 BubblePoint: Intake @70.2psia, Outlet @17.3psia @13:55:43.86 SS.reconfigure SSconfig @13:55:43.89 SS.seek 10.00ml @13:55:49.82 delay 15 seconds @13:56:04.92 Evacuated: Intake @68.6psia, Outlet @0.0psia SSV.dial :spit,avoiding => exhaust @13:56:06.24 SS.reconfigure SSexhale @13:56:06.27 SS.seek 0.00ml @13:56:11.16 SS.reconfigure SSfast @13:56:11.20 SS.seek 5.00ml @13:56:14.10 SSV.dial CBV,avoiding => exhaust @13:56:14.12
CBV.dial SSV @13:56:15.42 CTV.dial :air,avoiding => intake @13:56:16.73 SS.reconfigure SSconfig @13:56:16.75 CSV.dial CTV @13:56:16.79
SS.seek 10.00ml @13:56:17.76 CSV.dial :air @13:56:18.26 CS.reconfigure CSfast @13:56:18.29 CS.seek 0.000ml @13:56:19.97 CS.coast @13:56:20.16
SSV.dial :spit,avoiding => exhaust @13:56:21.47 SS.reconfigure SSexhale @13:56:21.50 SS.seek 0.00ml @13:56:26.62 SS.reconfigure SSfast @13:56:26.65 SS.seek 5.00ml @13:56:29.55 SSV.dial CBV,avoiding => exhaust @13:56:29.57
CBV.dial SSV @13:56:31.87 SSV.dial :spit,avoiding => exhaust @13:56:33.18 SS.reconfigure SSconfig @13:56:33.21 SS.seek 0.00ml @13:56:36.43 SS.coast @13:56:36.46 Evacuating Puck (bubblePt=55.0psi, delay=15.0s) Intake.select :close @13:56:36.52 CSV.dial :waste @13:56:36.61
SSV.dial :exhaust @13:56:37.28 CS.seek 0.000ml @13:56:37.37
Exhaust.select :open @13:56:37.48 SS.reconfigure SSexhale @13:56:37.52 SS.seek 0.00ml @13:56:37.56 CSV.dial :air @13:56:38.04
Exhaust.select :close @13:56:38.13 SSV.dial CBV,avoiding => exhaust @13:56:38.61
CBV.dial SSV @13:56:38.64 CS.seek 10.000ml @13:56:40.49
CTV.dial :air @13:56:40.50 delay 15 seconds @13:56:55.50 SSV.dial :spit,avoiding => exhaust @13:56:56.81 CTV.dial CSV,avoiding => intake @13:56:56.83
SS.seek 0.00ml @13:56:56.93 SSV.dial CBV,avoiding => exhaust @13:57:02.10 CBV.dial between CSV and waste2b @13:57:03.03 CSV.dial between air and CTV @13:57:03.95 CS.seek 8.290ml @13:57:08.65 CTV.dial CSV,avoiding => air @13:57:08.66
CSV.dial CTV @13:57:09.04 CS.reconfigure CSpressurize @13:57:09.07 CS.seek 0.000ml @13:57:30.64 CSV.dial :air @13:57:31.15 CS.reconfigure CSfast @13:57:31.18 CS.seek 10.000ml @13:57:54.37 CSV.dial between air and CTV @13:57:54.74 CS.seek 2.934ml @13:58:11.46 CTV.dial CSV,avoiding => air @13:58:11.47
CSV.dial CTV @13:58:11.76 CS.reconfigure CSpressurize @13:58:11.79 CS.seek 0.000ml @13:58:18.00 BubblePoint: Intake @70.0psia, Outlet @14.9psia @13:58:18.01 SS.reconfigure SSconfig @13:58:18.04 SS.seek 10.00ml @13:58:24.03 delay 15 seconds @13:58:39.13 Evacuated: Intake @69.0psia, Outlet @0.1psia @13:58:39.14 SSV.dial :spit,avoiding => exhaust @13:58:40.45 SS.reconfigure SSexhale @13:58:40.48 SS.seek 0.00ml @13:58:45.53 SS.reconfigure SSfast @13:58:45.57 SS.seek 5.00ml @13:58:48.51 SSV.dial CBV,avoiding => exhaust @13:58:48.53
CBV.dial SSV @13:58:49.84 CTV.dial :air,avoiding => intake @13:58:51.14 CSV.dial CTV @13:58:51.16
SS.reconfigure SSconfig @13:58:51.20 SS.seek 10.00ml @13:58:52.16 CSV.dial :air @13:58:52.68 CS.reconfigure CSfast @13:58:52.71 CS.seek 0.000ml @13:58:54.50
SSV.dial :spit,avoiding => exhaust @13:58:55.07 CS.coast @13:58:55.81
SS.reconfigure SSexhale @13:58:55.84 SS.seek 0.00ml @13:59:01.08 SS.reconfigure SSfast @13:59:01.11 SS.seek 5.00ml @13:59:04.06 SSV.dial CBV,avoiding => exhaust @13:59:04.07
CBV.dial SSV @13:59:06.37 SSV.dial :spit,avoiding => exhaust @13:59:07.68 SS.reconfigure SSconfig @13:59:07.71 SS.seek 0.00ml @13:59:11.00 SS.coast @13:59:11.03 Lysing: Adding 1.5ml lysis CBV.dial between CSV and waste1,avoiding => DBV @13:59:12.50 CTV.dial CSV @13:59:13.74 CSV.dial CSR @13:59:14.52 CSR.select :lysis @13:59:14.62 CS.reconfigure CSconfig @13:59:14.66 CS.seek 1.000ml @13:59:17.95 delay 5 seconds @13:59:22.96 CSR.select :air @13:59:23.10 CS.seek 6.000ml @13:59:36.54 delay 2 seconds @13:59:38.55 CSV.dial :waste1 @13:59:39.81 CS.reconfigure CSfast @13:59:39.84 CS.seek empty @13:59:54.04 CSV.dial CSR @13:59:55.30 CSR.select :lysis @13:59:55.40 CS.reconfigure CSconfig @13:59:55.44 CS.seek 1.500ml @14:00:00.16 delay 5 seconds @14:00:05.18 CSR.select :air @14:00:05.32 CS.seek 4.500ml @14:00:13.73 delay 2 seconds @14:00:15.75 CSV.dial CTV @14:00:16.27 CS.seek 1.275ml @14:00:25.27 Heating sh1 puck from tube 2 in Collection Clamp to 85.0C for 5:00 @14:00:25.28 CH.seek 85.0C,8:00 @14:03:00.83 CH.hold 5:00,85.0C @14:08:00.93 Collection Puck Heater at 85.0C after lysing @14:08:00.94 CS.seek 0.000ml @14:08:04.93 CTV.dial between spare and air,avoiding => intake @14:08:05.92 CSV.dial :air @14:08:06.35 CS.seek 5.000ml @14:08:19.80 CSV.dial CTV @14:08:20.30 delay 2 seconds @14:08:22.31 CSV.dial :air @14:08:22.81 delay 2 seconds @14:08:24.82 CSV.dial CTV @14:08:25.31 delay 2 seconds @14:08:27.32 CSV.dial :air @14:08:27.81 delay 2 seconds @14:08:29.82 CS.seek 0.000ml @14:08:43.33 Recovering Lysate into Collection syringe @14:08:43.34 CSV.dial CBV @14:08:44.33 CBV.dial CSV @14:08:44.67 CS.seek 5.000ml @14:08:58.14 delay 5 seconds @14:09:03.15 CTV.dial :air @14:09:03.64 CS.seek 7.000ml @14:09:09.48 delay 5 seconds @14:09:14.49 CSV.dial :air2 @14:09:14.91 CS.seek 10.000ml @14:09:23.26 CSV.dial between air and CTV,avoiding => CBV @14:09:24.38 Lysing Complete @14:09:24.43 Storing sh1 puck in Collection Clamp to tube 1 @14:09:24.56 SC.reconfigure SCtight @14:09:24.59 SC.seek 1 @14:09:26.86 SC.reconfigure SCloose @14:09:27.63 Elbow.reconfigure TightElbow @14:09:27.66 Elbow.seek 1 @14:09:29.29 Forearm.reconfigure TightForearm @14:09:29.32 Forearm.seek 1 + 800 counts @14:09:30.48 SE.reconfigure SEpick @14:09:30.53 SE.seek beamBreak @14:09:33.32 SE.configure SEconfig @14:09:33.36 SE.seek place @14:09:33.79 Forearm.reconfigure LooseForearm @14:09:33.83 Forearm.seek retract @14:09:34.78 Elbow.seek CC @14:09:36.34 Elbow.reconfigure LooseElbow @14:09:36.43 Forearm.seek CC @14:09:37.38 CC.seek sealed @14:09:38.29 Hand.grip @14:09:39.03 CC.seek open @14:09:42.39 Forearm.seek retract @14:09:43.35 Elbow.reconfigure TightElbow @14:09:43.39 Elbow.seek 1 @14:09:44.96 Elbow.reconfigure LimpElbow @14:09:45.00 Forearm.seek 1 @14:09:46.02 Elbow.hold @14:09:46.07 Elbow.reconfigure TightElbow @14:09:46.11 Hand.release @14:09:46.86 Forearm.coast @14:09:46.89 Forearm.seek 1 + 331 counts @14:09:47.12 Forearm.reconfigure TightForearm @14:09:47.15 Forearm.seek 1 + 800 counts @14:09:47.35 SE.seek down @14:09:48.45
Forearm.reconfigure LooseForearm @14:09:48.49 Forearm.seek retract @14:09:50.07 SE.coast @14:09:50.10
SC.coast @14:09:50.12 Tranferring lysate to PRV @14:09:50.13 CSV.dial :air @14:09:50.44 CS.seek 10.000ml @14:09:50.48 CSV.dial between air and CTV @14:09:50.86 AIV.dial :bypass @14:09:51.02 Beginning samplePassC @14:09:51.03 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:09:51.17 Elbow.seek FlushPuck::Garage @14:09:52.95 Elbow.reconfigure LooseElbow @14:09:53.03 Forearm.seek garage @14:09:54.08 Hand.grip @14:09:54.86 Forearm.seek retract @14:09:55.87 Elbow.reconfigure TightElbow @14:09:55.91 Elbow.seek CC @14:09:58.66 Elbow.reconfigure LooseElbow @14:09:58.75 Forearm.reconfigure TightForearm @14:09:58.78 Forearm.seek CC @14:09:59.90 CC.seek closed @14:10:03.64 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 2.04 @14:10:03.70 Hand.release @14:10:04.45 Forearm.reconfigure LooseForearm @14:10:04.48 Forearm.seek retract @14:10:05.41 AWV.select :air @14:10:05.42 AIV.dial :bypass @14:10:05.43 PSV.dial PSR @14:10:06.72 PSR.select :flush @14:10:06.82 PS.seek 1.500ml @14:10:22.92 delay 5 seconds @14:10:27.94 PSV.dial :waste1 @14:10:28.38 PS.seek 0.000ml @14:10:44.39 PSV.dial PSR @14:10:44.84 PSR.select :air @14:10:44.94 PS.seek 2.500ml @14:11:11.12 CBV.dial PRV @14:11:11.13 PRV.dial CBV @14:11:12.44
PSV.dial :mixloop @14:11:13.44 CTV.dial :air,avoiding => intake @14:11:13.45 PTV.dial :PRVmixing @14:11:13.52 PS.seek 0.000ml @14:11:39.73 PSV.dial :air @14:11:40.18 PS.seek 2.500ml @14:12:06.40 PSV.dial :mixloop @14:12:06.84 PS.seek 0.000ml @14:12:33.15 PSV.dial :air @14:12:33.59 PS.seek 2.500ml @14:12:59.86 PSV.dial :mixloop @14:13:00.31 PS.seek 0.000ml @14:13:26.49 Garaging FlushPuck in Collection Clamp @14:13:26.55 Elbow.reconfigure TightElbow @14:13:26.58 Elbow.seek CC @14:13:26.67 Elbow.reconfigure LooseElbow @14:13:26.76 Forearm.seek CC @14:13:27.74 CC.seek sealed @14:13:28.67 Hand.grip @14:13:29.41 CC.seek open @14:13:32.69 Forearm.seek retract @14:13:33.63 Elbow.reconfigure TightElbow @14:13:33.67 Elbow.seek garage @14:13:36.45 Elbow.reconfigure LooseElbow @14:13:36.53 Forearm.reconfigure TightForearm @14:13:36.56 Forearm.seek garage @14:13:37.76 Hand.release @14:13:38.57 Forearm.reconfigure LooseForearm @14:13:38.60 Forearm.seek retract @14:13:39.65 Connecting PS to CS to transfer sample @14:13:39.66 CBV.dial AIV @14:13:39.67 AIV.dial :bypass @14:13:39.68 PSV.dial :mixloop @14:13:39.69 PTV.dial :PRVmixing @14:13:39.70 PRV.dial AIV @14:13:39.71 CTV.dial :air,avoiding => intake @14:13:39.72 Loading lyfil puck from tube 2 into Collection Clamp @14:13:39.89 SC.reconfigure SCtight @14:13:39.92 SC.seek 2 @14:13:42.23 SC.reconfigure SCloose @14:13:42.40 Elbow.reconfigure TightElbow @14:13:42.43 Elbow.seek 2 @14:13:44.21 Forearm.reconfigure TightForearm @14:13:44.24 Forearm.seek 2 + 800 counts @14:13:45.29 SE.reconfigure SEpick @14:13:45.34 SE.seek beamBreak @14:13:48.18 SE.configure SEconfig @14:13:48.30 Elbow.seek 2 @14:13:48.44 Forearm.seek 2 + 300 counts @14:13:48.74 Forearm.reconfigure LooseForearm @14:13:48.78 Hand.grip @14:13:49.54 SE.seek down @14:13:49.68
Forearm.seek retract @14:13:50.64 Elbow.seek CC @14:13:52.16 Elbow.reconfigure LooseElbow @14:13:52.22 SE.coast @14:13:52.29
Forearm.reconfigure TightForearm @14:13:52.32 Forearm.seek CC @14:13:53.41 CC.seek closed @14:13:57.18 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 2.04 @14:13:57.19 Hand.release @14:13:57.95 Forearm.reconfigure LooseForearm @14:13:57.98 Forearm.seek retract @14:13:58.91 SC.coast @14:13:58.94 CTV.dial CSV,avoiding => intake @14:14:00.21 CSV.dial CTV @14:14:00.50 CS.seek 5.000ml @14:14:13.91 delay 1:20 @14:15:33.92 CS.seek 2.500ml @14:15:41.07 delay 40 seconds @14:16:21.08 CS.seek 0.000ml @14:16:28.16 delay 1:00 @14:17:28.17 PSV.dial :air @14:17:28.63 PS.seek 2.500ml @14:17:54.85 PSV.dial :mixloop @14:17:55.41 delay 3 seconds @14:17:58.42 PSV.dial :air @14:17:58.96 delay 2 seconds @14:18:00.97 PSV.dial :mixloop @14:18:01.93 delay 3 seconds @14:18:04.94 PSV.dial :waste1 @14:18:06.24 PS.seek empty @14:18:32.51 CBV.dial :waste1 @14:18:32.98 delay 5 seconds @14:18:37.99 CBV.dial AIV @14:18:38.45 delay 10 seconds @14:18:48.46 CBV.dial :waste1 @14:18:48.92 delay 10 seconds @14:18:58.93 CBV.dial AIV @14:18:59.38 delay 10 seconds @14:19:09.39 CTV.dial CSV,avoiding => intake @14:19:09.40 CBV.dial between AIV and waste1 @14:19:09.73 CSV.dial :air @14:19:10.24 CS.seek 10.000ml @14:19:36.34 CSV.dial CTV @14:19:36.85 CS.seek 0.000ml @14:20:02.96 CBV.dial :waste1 @14:20:03.26 delay 10 seconds @14:20:13.27 CTV.dial :air,avoiding => intake @14:20:14.60 Storing lyfil puck in Collection Clamp to tube 1 @14:20:14.72 SC.reconfigure SCtight @14:20:14.75 SC.seek 1 @14:20:16.99 SC.reconfigure SCloose @14:20:17.15 Elbow.reconfigure TightElbow @14:20:17.18 Elbow.seek 1 @14:20:18.75 Forearm.reconfigure TightForearm @14:20:18.78 Forearm.seek 1 + 800 counts @14:20:19.85 SE.reconfigure SEpick @14:20:19.90 SE.seek beamBreak @14:20:22.81 SE.configure SEconfig @14:20:22.94 Elbow.reconfigure LimpElbow @14:20:22.97 Forearm.reconfigure LooseForearm @14:20:23.01 Forearm.seek 1 @14:20:23.25 Elbow.hold @14:20:23.30 Elbow.reconfigure TightElbow @14:20:23.38 SE.seek 0.04 Pucks below Top Plate @14:20:23.68 Forearm.coast @14:20:23.71 Forearm.seek 1 + 346 counts @14:20:23.94 Forearm.reconfigure TightForearm @14:20:23.98 Forearm.seek 1 + 800 counts @14:20:24.28 SE.seek 1.04 Pucks below Top Plate @14:20:24.83 Forearm.reconfigure LooseForearm @14:20:24.86 Forearm.seek retract @14:20:25.78 Elbow.seek CC @14:20:27.40 Elbow.reconfigure LooseElbow @14:20:27.49 Forearm.seek CC @14:20:28.45 CC.seek sealed @14:20:29.40 Hand.grip @14:20:30.14 CC.seek open @14:20:33.46 Forearm.seek retract @14:20:34.44 Elbow.reconfigure TightElbow @14:20:34.48 Elbow.seek 1 @14:20:36.11 Elbow.reconfigure LimpElbow @14:20:36.15 Forearm.seek 1 @14:20:37.17 Elbow.hold @14:20:37.22 Elbow.reconfigure TightElbow @14:20:37.26 Hand.release @14:20:38.08 Forearm.coast @14:20:38.12 Forearm.seek 1 + 327 counts @14:20:38.35 Forearm.reconfigure TightForearm @14:20:38.38 Forearm.seek 1 + 800 counts @14:20:38.69 SE.seek down @14:20:39.80
Forearm.reconfigure LooseForearm @14:20:39.84 Forearm.seek retract @14:20:41.34 SE.coast @14:20:41.37
SC.coast @14:20:41.39 PSV.dial :air @14:20:42.46 PS.seek 0.499ml @14:20:48.44 delay 3 seconds @14:20:51.45 PSV.dial :mixloop @14:20:51.89 PRV.dial :air @14:20:52.33 delay 3 seconds @14:20:55.34 PRV.dial CBV @14:20:55.78 CBV.dial AIV @14:20:56.24 PRV.dial between CBV and air @14:20:56.55 PRV.dial :air @14:20:56.88 PS.reconfigure PSslow @14:20:56.92 PS.seek 0.599ml @14:20:59.47 PRV.dial :waste2 @14:21:00.20 PS.reconfigure PSconfig @14:21:00.23 PS.seek empty @14:21:07.66 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:21:07.79 Elbow.seek FlushPuck::Garage @14:21:09.57 Elbow.reconfigure LooseElbow @14:21:09.66 Forearm.seek garage @14:21:10.67 Hand.grip @14:21:11.48 Forearm.seek retract @14:21:12.50 Elbow.reconfigure TightElbow @14:21:12.54 Elbow.seek CC @14:21:15.30 Elbow.reconfigure LooseElbow @14:21:15.38 Forearm.reconfigure TightForearm @14:21:15.41 Forearm.seek CC @14:21:16.44 CC.seek closed @14:21:20.24 Collection Clamp onSpring/offSpring for 5/22 samples, on/off torque 2.04 @14:21:20.29 Hand.release @14:21:21.05 Forearm.reconfigure LooseForearm @14:21:21.08 Forearm.seek retract @14:21:22.07 Clearing PML for modified lysate CTV.dial :air,avoiding => intake @14:21:22.08 PRV.dial :waste2 @14:21:22.09 PTV.dial :PRV,avoiding => puck @14:21:22.82 delay 2 seconds @14:21:24.82 PTV.dial :PRVmixing,avoiding => puck @14:21:25.54 delay 2 seconds @14:21:27.55 PSV.dial :PML @14:21:27.56 delay 2 seconds @14:21:29.56 PRV.dial :air @14:21:30.29 delay 2 seconds @14:21:32.35 PS.seek 1.499ml @14:21:48.43 delay 5 seconds @14:21:53.45 PS.seek 2.500ml @14:22:04.62 PSV.dial :air @14:22:05.07 PS.seek 0.000ml @14:22:31.34 mixing lysate with speDil @14:22:31.35 PSV.dial :PML @14:22:31.79 CBV.dial :PRV @14:22:31.80 PRV.dial :air @14:22:31.81 delay 2 seconds @14:22:33.82 PRV.dial :speDil @14:22:34.26 delay 2 seconds @14:22:36.32 PS.reconfigure PSslow1 @14:22:36.35 PS.seek 0.225ml @14:22:59.65 delay 5 seconds @14:23:04.66 PRV.dial CBV @14:23:05.40 delay 2 seconds @14:23:07.46 PS.seek 0.475ml @14:23:33.56 delay 5 seconds @14:23:38.57 PRV.dial :air @14:23:39.01 delay 2 seconds @14:23:41.02 PS.seek 0.925ml @14:24:27.08 delay 10 seconds @14:24:37.09 PRV.dial CBV @14:24:37.54 CTV.dial :air,avoiding => intake @14:24:37.55 PSV.dial :air @14:24:38.02 PS.reconfigure PSconfig @14:24:38.05 PS.seek 2.500ml @14:24:54.90 delay 3 seconds @14:24:57.92 PSV.dial PTV @14:24:58.65 PTV.dial :PRV,avoiding => puck @14:24:59.39 PS.seek 0.000ml @14:25:25.68 PSV.dial :air @14:25:26.42 PS.seek 2.500ml @14:25:52.71 delay 3 seconds @14:25:55.73 PSV.dial PTV @14:25:56.46 PTV.dial :PRV,avoiding => puck @14:25:56.48 PS.seek 0.000ml @14:26:22.74 Pushing modified lysate into AIV Injection Loop @14:26:22.75 CBV.dial AIV @14:26:22.76 AIV.dial :bypass @14:26:22.77 PRV.dial CBV @14:26:22.78 PSV.dial :air @14:26:23.53 PS.seek 2.500ml @14:26:49.70 delay 3 seconds @14:26:52.71 PSV.dial :PML @14:26:53.16 PTV.dial :PRVmixing,avoiding => puck @14:26:53.94 PS.reconfigure PSslow1 @14:26:53.98 PS.seek 1.850ml @14:28:00.64 delay 5 seconds @14:28:05.65 AIV.dial :sample @14:28:06.17 AWV.select :waste @14:28:06.31 PS.reconfigure PSslow2 @14:28:06.34 PS.seek 1.310ml @14:29:56.61 delay 15 seconds @14:30:11.62 AIV.dial :bypass @14:30:12.28 PS.reconfigure PSconfig @14:30:12.31 PS.seek 0.000ml @14:30:26.45 PSV.dial :air @14:30:26.92 PS.seek 2.500ml @14:30:53.17 PSV.dial :PML @14:30:54.17 PS.seek 0.000ml @14:31:02.34 Session Begun @14:31:12.65 -> showlog @14:31:20.61
AIV.dial :coilToMFB @14:31:20.62 AWV.select :air @14:31:20.77 Extracting Nucleic Acid from AIV @14:31:20.79 SPE.hold 2:46:40,55.0C @14:31:20.81
Beginning samplePassD @14:31:20.84 ASV.dial :SPEdelivery @14:31:20.97
AWV.select :air @14:31:20.98 AIV.dial :bypass @14:31:21.00 PSV.dial PSR @14:31:21.40 delay 20 seconds @14:31:22.06
PSR.select :killMFB @14:31:22.17 PS.seek 1.500ml @14:31:38.23 delay 5 seconds @14:31:41.41 primeLysateINT @14:31:41.42 AWV.select :air @14:31:41.43 AIV.dial :coilToMFB @14:31:41.45 ATV.dial :AIV @14:31:42.81 AS.reconfigure ASslow1 @14:31:42.86 AS.seek 0.239ml @14:31:43.24
PSV.dial :waste1 @14:31:43.71 PS.seek 0.000ml @14:31:59.83 PSV.dial PSR @14:32:00.31 PSR.select :air @14:32:00.42 PS.seek 2.500ml @14:32:26.64 PRV.dial CBV @14:32:26.66
CBV.dial PRV @14:32:26.67 PSV.dial :mixloop @14:32:27.75 CTV.dial :air,avoiding => intake @14:32:27.76 PTV.dial :PRVmixing @14:32:27.79 PS.seek 0.000ml @14:32:32.13 delay 10 seconds @14:32:42.14 ATV.dial :air2 @14:32:42.73 AS.seek 0.269ml @14:32:49.08 ATV.dial :waste2 @14:32:49.45 AS.reconfigure ASnorm @14:32:49.49 AS.seek empty @14:32:54.04
PSV.dial PSR @14:32:55.12 PSR.select :flush @14:32:55.24 PS.seek 1.500ml @14:33:03.66 Lysate primed! @14:33:03.67 AFV.select :holdingCoil @14:33:03.68 ATV.dial :air2 @14:33:04.10 AS.reconfigure ASslow1 @14:33:04.15 AS.seek 0.069ml @14:33:11.32
delay 5 seconds @14:33:16.33 PSV.dial :waste1 @14:33:16.80 PS.seek 0.000ml @14:33:18.76 delay 3 seconds @14:33:21.76 ATV.dial :AIV @14:33:22.43 AS.seek 0.469ml @14:33:32.88
PSV.dial PSR @14:33:33.34 PSR.select :air @14:33:33.45 PS.seek 2.500ml @14:33:59.73 PRV.dial CBV @14:33:59.75
CBV.dial PRV @14:33:59.76 PSV.dial :mixloop @14:34:00.82 CTV.dial :air,avoiding => intake @14:34:00.83 PTV.dial :PRVmixing @14:34:00.85 PS.seek 0.000ml @14:34:27.13 PSV.dial PSR @14:34:28.18 PSR.select :flush @14:34:28.29 PS.seek 1.500ml @14:34:44.31 delay 5 seconds @14:34:44.36
delay 5 seconds @14:34:49.36 Solid Phase Extraction Heater at 54.0C @14:34:49.37 ATV.dial ASV @14:34:49.43
PSV.dial :waste1 @14:34:49.83 ASV.dial :SPEdelivery @14:34:49.91 AS.seek 0.041ml @14:34:49.94
PS.seek 0.000ml @14:35:06.10 PSV.dial PSR @14:35:06.57 PSR.select :air @14:35:06.68 PS.seek 2.500ml @14:35:32.88 PRV.dial CBV @14:35:32.90
CBV.dial PRV @14:35:32.93 PSV.dial :mixloop @14:35:33.96 CTV.dial :air,avoiding => intake @14:35:33.97 PTV.dial :PRVmixing @14:35:34.01 PS.seek 0.000ml @14:36:00.44 PSV.dial PSR @14:36:01.47 PSR.select :flush @14:36:01.58 PS.seek 1.500ml @14:36:17.62 delay 10 seconds @14:36:17.78
delay 5 seconds @14:36:22.79 PSV.dial :waste1 @14:36:23.25 PS.seek 0.000ml @14:36:27.63 rinseLysateLineINT @14:36:27.64 AFV.select :carrierSolution @14:36:27.72 -> exit @14:36:27.73 Session Ended @14:36:27.79 delay 2 seconds @14:36:29.80 AS.reconfigure ASconfig @14:36:29.83 AS.seek 1.000ml @14:36:39.28
PSV.dial PSR @14:36:39.72 PSR.select :air @14:36:39.82 PS.seek 2.500ml @14:37:06.13 PRV.dial CBV @14:37:06.15
CBV.dial PRV @14:37:06.16 PSV.dial :mixloop @14:37:07.17 CTV.dial :air,avoiding => intake @14:37:07.18 PTV.dial :PRVmixing @14:37:07.19 PS.reconfigure PSslow @14:37:07.23 PS.seek 1.200ml @14:37:25.99 delay 1 second @14:37:27.00 AFV.select :holdingCoil @14:37:27.09 delay 1 second @14:37:28.10 ATV.dial :AIV @14:37:28.43 AIV.dial :coilToMFB @14:37:28.44 AWV.select :waste @14:37:28.53 AS.reconfigure ASslow0 @14:37:28.57 AS.seek 0.000ml @14:37:34.09
PSV.dial :waste1 @14:37:35.42 PS.reconfigure PSconfig @14:37:35.45 PS.seek 0.000ml @14:37:48.50 PSV.dial :air @14:37:49.54 PS.seek 2.500ml @14:38:15.75 PSV.dial :mixloop @14:38:16.20 PS.reconfigure PSslow @14:38:16.23 PS.seek 0.000ml @14:39:07.56 PSV.dial :air @14:39:08.02 PS.reconfigure PSconfig @14:39:08.05 PS.seek 2.500ml @14:39:11.36 ATV.dial :air2 @14:39:11.93 AS.reconfigure ASslow1 @14:39:11.96 AS.seek 0.050ml @14:39:22.47 ATV.dial :AIV @14:39:23.04 AS.seek 0.010ml @14:39:31.57 ATV.dial :waste @14:39:32.84 AS.seek empty @14:39:34.34
PSV.dial :mixloop @14:39:34.80 PS.reconfigure PSslow @14:39:34.83 PS.seek 0.000ml @14:39:35.41 ethanol wash ATV.dial :air2 @14:39:36.35 AS.reconfigure ASslow2 @14:39:36.38 AS.seek 0.009ml @14:39:47.10 delay 3 seconds @14:39:50.11 Pulling 0.4ml of Ethanol Wash @14:39:50.12 ATV.dial :ethanol @14:39:50.77 AS.reconfigure ASslow1 @14:39:50.80 AS.seek 0.409ml @14:40:26.12
Cleaning Collection Syringe @14:40:26.13 CSV.dial CSR @14:40:26.55 CSR.select :flush @14:40:26.65 CS.seek 4.000ml @14:40:37.66 delay 5 seconds @14:40:42.67 CSR.select :air @14:40:42.76 CS.seek 10.000ml @14:40:58.69 CSV.dial CTV @14:40:58.71
CTV.dial CSV @14:41:00.04 CBV.dial :waste1 @14:41:00.53 CS.seek 0.000ml @14:41:12.63 delay 3 seconds @14:41:15.64 ATV.dial :air1 @14:41:15.97 delay 3 seconds @14:41:19.03 AS.seek 0.434ml @14:41:24.38 delay 3 seconds @14:41:26.69
CSV.dial CSR @14:41:27.19 CSR.select :flush @14:41:27.29 CS.seek 4.000ml @14:41:27.39 ATV.dial :ethanol @14:41:27.77 AS.seek 0.419ml @14:41:31.06 delay 3 seconds @14:41:34.07 ATV.dial ASV @14:41:35.19 AS.seek 0.092ml @14:41:38.28
delay 5 seconds @14:41:43.29 CSR.select :air @14:41:43.38 CS.seek 10.000ml @14:41:59.35 CSV.dial CTV @14:41:59.37
CTV.dial CSV @14:41:59.88 CBV.dial :waste1 @14:41:59.90 CS.seek 0.000ml @14:42:26.18 Garaging FlushPuck in Collection Clamp @14:42:26.23 Elbow.reconfigure TightElbow @14:42:26.26 Elbow.seek CC @14:42:26.36 Elbow.reconfigure LooseElbow @14:42:26.44 Forearm.seek CC @14:42:27.41 CC.seek sealed @14:42:28.34 Hand.grip @14:42:29.09 CC.seek open @14:42:32.40 Forearm.seek retract @14:42:33.37 Elbow.reconfigure TightElbow @14:42:33.41 Elbow.seek garage @14:42:36.17 Elbow.reconfigure LooseElbow @14:42:36.25 Forearm.reconfigure TightForearm @14:42:36.28 Forearm.seek garage @14:42:37.45 Hand.release @14:42:38.25 Forearm.reconfigure LooseForearm @14:42:38.29 Forearm.seek retract @14:42:39.35 Whole Cell Archive sampling 1000ml @14:42:39.36 Loading wcr puck from tube 2 into Collection Clamp @14:42:39.53 SC.reconfigure SCtight @14:42:39.56 SC.seek 2 @14:42:41.81 SC.reconfigure SCloose @14:42:41.98 Elbow.reconfigure TightElbow @14:42:42.02 Elbow.seek 2 @14:42:42.15 delay 3 seconds @14:42:43.86 Forearm.reconfigure TightForearm @14:42:43.89 Forearm.seek 2 + 800 counts @14:42:44.95 SE.reconfigure SEpick @14:42:45.54 ATV.dial :waste2 @14:42:45.56 SE.seek beamBreak @14:42:46.18 AS.reconfigure ASconfig @14:42:46.21 AS.seek empty @14:42:48.48 SE.configure SEconfig @14:42:48.61 Elbow.seek 2 @14:42:48.75 Forearm.seek 2 + 300 counts @14:42:48.95 Forearm.reconfigure LooseForearm @14:42:48.99 Hand.grip @14:42:49.73 SE.seek down @14:42:49.85 Forearm.seek retract @14:42:50.82 Elbow.seek CC @14:42:51.94 ATV.dial :air2 @14:42:52.36 AS.reconfigure ASslow1 @14:42:52.40 Elbow.reconfigure LooseElbow @14:42:52.43 AS.seek 1.000ml @14:42:52.55 SE.coast @14:42:52.57 Forearm.reconfigure TightForearm @14:42:52.63 Forearm.seek CC @14:42:53.74 CC.seek closed @14:42:57.52 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.98 @14:42:57.53 Hand.release @14:42:58.25 Forearm.reconfigure LooseForearm @14:42:58.28 Forearm.seek retract @14:42:59.23 SC.coast @14:42:59.26 collectSample 1000ml w/bubble point of 55psi @14:42:59.28 Priming 7ml Intake.select :close @14:42:59.29 Exhaust.select :close @14:42:59.30 SS.hold @14:42:59.46 Calibrating pressure sensors @14:42:59.47 CTV.dial :air @14:43:00.73 SSV.dial :spit,avoiding => exhaust @14:43:00.74 SS.reconfigure SSfast @14:43:00.77 SS.seek 5.00ml @14:43:03.41 SSV.dial CBV @14:43:03.43 CBV.dial SSV @14:43:04.93 delay 3.8 seconds @14:43:08.83 SSV.dial :spit @14:43:10.14 SS.seek 0.00ml @14:43:12.86 SSV.dial CBV @14:43:14.14 CTV.dial :intake @14:43:14.87 SSV.dial CBV @14:43:14.88 CBV.dial SSV @14:43:14.89 Allowing 55s for 7.0ml of remaining 7.0ml @14:43:14.90 Intake.select :open @14:43:17.03 Intake @33.0psia, vacuum from 20.0 to 24.0psi @14:43:17.05 SS.reconfigure forSampling @14:43:17.08 SS.seek 7.00ml @14:43:22.75 Stroke completed -- waiting for pressure to equalize ... @14:43:23.39 Intake.select :close @14:43:23.53 SSV.dial :exhaust @14:43:24.28 Exhaust.select :open @14:43:24.38 SS.reconfigure SSexhale @14:43:24.42 SS.seek 0.00ml @14:43:27.91 Exhaust.select :close @14:43:28.01 Sampling 1000.0ml @14:43:28.02 Intake.select :close @14:43:28.03 Exhaust.select :close @14:43:28.04 SS.reconfigure SSconfig @14:43:28.07 SS.hold @14:43:28.23 CTV.dial :intake @14:43:28.24 SSV.dial CBV @14:43:28.26 CBV.dial SSV @14:43:29.04 Allowing 185s for 25.0ml of remaining 1000.0ml @14:43:29.05 Intake.select :open @14:43:31.18 Intake @33.1psia, vacuum from 20.0 to 24.0psi @14:43:31.20 SS.reconfigure forSampling @14:43:31.23 SS.seek 25.00ml @14:43:45.34 Stroke completed -- waiting for pressure to equalize ... @14:43:45.98 Intake.select :close @14:43:46.13 SSV.dial :exhaust @14:43:46.89 Exhaust.select :open @14:43:46.99 SS.reconfigure SSexhale @14:43:47.03 SS.seek 0.00ml @14:43:55.78 Exhaust.select :close @14:43:55.88 SSV.dial CBV @14:43:55.90 CBV.dial SSV @14:43:56.67 Allowing 185s for 25.0ml of remaining 975.0ml @14:43:56.68 Intake.select :open @14:43:58.82 Intake @32.8psia, vacuum from 20.0 to 24.0psi @14:43:58.84 SS.reconfigure forSampling @14:43:58.87 SS.seek 25.00ml @14:44:13.48 Stroke completed -- waiting for pressure to equalize ... @14:44:14.12 Intake.select :close @14:44:14.26 SSV.dial :exhaust @14:44:15.01 Exhaust.select :open @14:44:15.11 SS.reconfigure SSexhale @14:44:15.15 SS.seek 0.00ml @14:44:23.93 Exhaust.select :close @14:44:24.03 SSV.dial CBV @14:44:24.05 CBV.dial SSV @14:44:24.83 Allowing 185s for 25.0ml of remaining 950.0ml @14:44:24.84 Intake.select :open @14:44:26.98 Intake @33.2psia, vacuum from 20.0 to 24.0psi @14:44:27.00 SS.reconfigure forSampling @14:44:27.03 SS.seek 25.00ml @14:44:41.09 Stroke completed -- waiting for pressure to equalize ... @14:44:41.73 Intake.select :close @14:44:41.88 SSV.dial :exhaust @14:44:42.64 Exhaust.select :open @14:44:42.75 SS.reconfigure SSexhale @14:44:42.79 SS.seek 0.00ml @14:44:51.50 Exhaust.select :close @14:44:51.60 SSV.dial CBV @14:44:51.62 CBV.dial SSV @14:44:52.39 Allowing 185s for 25.0ml of remaining 925.0ml @14:44:52.40 Intake.select :open @14:44:54.54 Intake @33.1psia, vacuum from 20.0 to 24.0psi @14:44:54.56 SS.reconfigure forSampling @14:44:54.59 SS.seek 25.00ml @14:45:08.43 Stroke completed -- waiting for pressure to equalize ... @14:45:09.07 Intake.select :close @14:45:09.22 SSV.dial :exhaust @14:45:09.98 Exhaust.select :open @14:45:10.08 SS.reconfigure SSexhale @14:45:10.12 SS.seek 0.00ml @14:45:18.65 Exhaust.select :close @14:45:18.75 SSV.dial CBV @14:45:18.77 CBV.dial SSV @14:45:19.54 Allowing 185s for 25.0ml of remaining 900.0ml @14:45:19.55 Intake.select :open @14:45:21.69 Intake @33.2psia, vacuum from 20.0 to 24.0psi @14:45:21.71 SS.reconfigure forSampling @14:45:21.74 SS.seek 25.00ml @14:45:35.96 Stroke completed -- waiting for pressure to equalize ... @14:45:36.60 Intake.select :close @14:45:36.74 SSV.dial :exhaust @14:45:37.49 Exhaust.select :open @14:45:37.59 SS.reconfigure SSexhale @14:45:37.63 SS.seek 0.00ml @14:45:46.18 Exhaust.select :close @14:45:46.28 SSV.dial CBV @14:45:46.30 CBV.dial SSV @14:45:47.07 Allowing 185s for 25.0ml of remaining 875.0ml @14:45:47.09 Intake.select :open @14:45:49.22 Intake @33.1psia, vacuum from 20.0 to 24.0psi @14:45:49.24 SS.reconfigure forSampling @14:45:49.27 SS.seek 25.00ml @14:46:03.52 Stroke completed -- waiting for pressure to equalize ... @14:46:04.16 Intake.select :close @14:46:04.29 SSV.dial :exhaust @14:46:05.05 Exhaust.select :open @14:46:05.15 SS.reconfigure SSexhale @14:46:05.19 SS.seek 0.00ml @14:46:13.84 Exhaust.select :close @14:46:13.94 SSV.dial CBV @14:46:13.96 CBV.dial SSV @14:46:14.74 Allowing 185s for 25.0ml of remaining 850.0ml @14:46:14.75 Intake.select :open @14:46:16.81 delay 5 seconds @14:46:16.89 Intake @33.2psia, vacuum from 20.0 to 24.0psi @14:46:16.91 SS.reconfigure forSampling @14:46:16.94 SS.seek 25.00ml @14:46:21.82 ATV.dial ASV @14:46:22.53 ASV.dial :SPEdelivery @14:46:22.55 AS.seek 0.020ml @14:46:30.77 Stroke completed -- waiting for pressure to equalize ... @14:46:31.41 Intake.select :close @14:46:31.55 SSV.dial :exhaust @14:46:32.30 Exhaust.select :open @14:46:32.41 SS.reconfigure SSexhale @14:46:32.45 SS.seek 0.00ml @14:46:40.97 Exhaust.select :close @14:46:41.08 SSV.dial CBV @14:46:41.10 CBV.dial SSV @14:46:41.87 Allowing 185s for 25.0ml of remaining 825.0ml @14:46:41.88 Intake.select :open @14:46:44.02 Intake @33.2psia, vacuum from 20.0 to 24.0psi @14:46:44.04 SS.reconfigure forSampling @14:46:44.07 SS.seek 25.00ml @14:46:58.33 Stroke completed -- waiting for pressure to equalize ... @14:46:58.97 Intake.select :close @14:46:59.11 SSV.dial :exhaust @14:46:59.87 Exhaust.select :open @14:46:59.98 SS.reconfigure SSexhale @14:47:00.02 SS.seek 0.00ml @14:47:08.95 Exhaust.select :close @14:47:09.06 SSV.dial CBV @14:47:09.08 CBV.dial SSV @14:47:09.84 Allowing 185s for 25.0ml of remaining 800.0ml @14:47:09.85 Intake.select :open @14:47:12.00 Intake @33.0psia, vacuum from 20.0 to 24.0psi @14:47:12.02 SS.reconfigure forSampling @14:47:12.05 SS.seek 25.00ml @14:47:26.14 Stroke completed -- waiting for pressure to equalize ... @14:47:27.28 Intake.select :close @14:47:27.42 SSV.dial :exhaust @14:47:28.18 Exhaust.select :open @14:47:28.28 SS.reconfigure SSexhale @14:47:28.32 SS.seek 0.00ml @14:47:37.14 Exhaust.select :close @14:47:37.24 SSV.dial CBV @14:47:37.26 CBV.dial SSV @14:47:38.03 Allowing 185s for 25.0ml of remaining 775.0ml @14:47:38.04 Intake.select :open @14:47:40.18 Intake @33.1psia, vacuum from 20.0 to 24.0psi @14:47:40.20 SS.reconfigure forSampling @14:47:40.23 SS.seek 25.00ml @14:47:54.46 Stroke completed -- waiting for pressure to equalize ... @14:47:55.10 Intake.select :close @14:47:55.25 SSV.dial :exhaust @14:47:56.01 Exhaust.select :open @14:47:56.12 SS.reconfigure SSexhale @14:47:56.16 SS.seek 0.00ml @14:48:04.81 Exhaust.select :close @14:48:04.91 SSV.dial CBV @14:48:04.93 CBV.dial SSV @14:48:05.70 Allowing 185s for 25.0ml of remaining 750.0ml @14:48:05.71 Intake.select :open @14:48:07.85 Intake @33.1psia, vacuum from 20.0 to 24.0psi @14:48:07.87 SS.reconfigure forSampling @14:48:07.90 SS.seek 25.00ml @14:48:22.12 Stroke completed -- waiting for pressure to equalize ... @14:48:22.76 Intake.select :close @14:48:22.90 SSV.dial :exhaust @14:48:23.66 Exhaust.select :open @14:48:23.75 SS.reconfigure SSexhale @14:48:23.79 SS.seek 0.00ml @14:48:32.49 Exhaust.select :close @14:48:32.60 SSV.dial CBV @14:48:32.62 CBV.dial SSV @14:48:33.38 Allowing 185s for 25.0ml of remaining 725.0ml @14:48:33.39 Intake.select :open @14:48:35.53 Intake @33.1psia, vacuum from 20.0 to 24.0psi @14:48:35.55 SS.reconfigure forSampling @14:48:35.58 SS.seek 25.00ml @14:48:49.40 Stroke completed -- waiting for pressure to equalize ... @14:48:50.04 Intake.select :close @14:48:50.19 SSV.dial :exhaust @14:48:50.95 Exhaust.select :open @14:48:51.06 SS.reconfigure SSexhale @14:48:51.10 SS.seek 0.00ml @14:48:59.76 Exhaust.select :close @14:48:59.87 SSV.dial CBV @14:48:59.89 CBV.dial SSV @14:49:00.66 Allowing 185s for 25.0ml of remaining 700.0ml @14:49:00.67 Intake.select :open @14:49:02.81 Intake @33.1psia, vacuum from 20.0 to 24.0psi @14:49:02.83 SS.reconfigure forSampling @14:49:02.86 SS.seek 25.00ml @14:49:16.62 Stroke completed -- waiting for pressure to equalize ... @14:49:17.26 Intake.select :close @14:49:17.41 SSV.dial :exhaust @14:49:18.16 Exhaust.select :open @14:49:18.26 SS.reconfigure SSexhale @14:49:18.30 SS.seek 0.00ml @14:49:26.94 Exhaust.select :close @14:49:27.04 SSV.dial CBV @14:49:27.06 CBV.dial SSV @14:49:27.84 Allowing 185s for 25.0ml of remaining 675.0ml @14:49:27.85 Intake.select :open @14:49:29.98 Intake @33.1psia, vacuum from 20.0 to 24.0psi @14:49:30.00 SS.reconfigure forSampling @14:49:30.03 SS.seek 25.00ml @14:49:42.70 delay 5 seconds @14:49:44.21 Stroke completed -- waiting for pressure to equalize ... @14:49:44.85 Intake.select :close @14:49:45.00 SSV.dial :exhaust @14:49:45.76 Exhaust.select :open @14:49:45.86 SS.reconfigure SSexhale @14:49:45.90 SS.seek 0.00ml @14:49:47.71 ATV.dial :air2 @14:49:48.48 AS.seek 0.520ml @14:49:54.83 Exhaust.select :close @14:49:54.94 SSV.dial CBV @14:49:54.96 CBV.dial SSV @14:49:55.72 Allowing 185s for 25.0ml of remaining 650.0ml @14:49:55.74 Intake.select :open @14:49:57.87 Intake @33.1psia, vacuum from 20.0 to 24.0psi @14:49:57.89 SS.reconfigure forSampling @14:49:57.92 SS.seek 25.00ml @14:50:11.99 Stroke completed -- waiting for pressure to equalize ... @14:50:12.63 Intake.select :close @14:50:12.76 SSV.dial :exhaust @14:50:13.52 Exhaust.select :open @14:50:13.63 SS.reconfigure SSexhale @14:50:13.67 SS.seek 0.00ml @14:50:22.47 Exhaust.select :close @14:50:22.58 SSV.dial CBV @14:50:22.60 CBV.dial SSV @14:50:23.36 Allowing 185s for 25.0ml of remaining 625.0ml @14:50:23.37 Intake.select :open @14:50:25.51 Intake @32.9psia, vacuum from 20.0 to 24.0psi @14:50:25.53 SS.reconfigure forSampling @14:50:25.56 SS.seek 25.00ml @14:50:39.61 Stroke completed -- waiting for pressure to equalize ... @14:50:40.73 Intake.select :close @14:50:40.88 SSV.dial :exhaust @14:50:41.64 Exhaust.select :open @14:50:41.74 SS.reconfigure SSexhale @14:50:41.78 SS.seek 0.00ml @14:50:50.38 Exhaust.select :close @14:50:50.48 SSV.dial CBV @14:50:50.50 CBV.dial SSV @14:50:51.27 Allowing 185s for 25.0ml of remaining 600.0ml @14:50:51.29 Intake.select :open @14:50:53.42 Intake @33.2psia, vacuum from 20.0 to 24.0psi @14:50:53.44 SS.reconfigure forSampling @14:50:53.47 SS.seek 25.00ml @14:51:07.59 Stroke completed -- waiting for pressure to equalize ... @14:51:08.23 Intake.select :close @14:51:08.37 SSV.dial :exhaust @14:51:09.13 Exhaust.select :open @14:51:09.23 SS.reconfigure SSexhale @14:51:09.27 SS.seek 0.00ml @14:51:17.97 Exhaust.select :close @14:51:18.07 SSV.dial CBV @14:51:18.09 CBV.dial SSV @14:51:18.86 Allowing 185s for 25.0ml of remaining 575.0ml @14:51:18.87 Intake.select :open @14:51:21.01 Intake @33.1psia, vacuum from 20.0 to 24.0psi @14:51:21.03 SS.reconfigure forSampling @14:51:21.06 SS.seek 25.00ml @14:51:30.71 delay 5 seconds @14:51:34.81 Stroke completed -- waiting for pressure to equalize ... @14:51:35.45 Intake.select :close @14:51:35.59 SSV.dial :exhaust @14:51:35.72 ATV.dial ASV @14:51:36.34 Exhaust.select :open @14:51:36.44 ASV.dial :wasteR @14:51:36.48 SS.reconfigure SSexhale @14:51:36.51 SS.seek 0.00ml @14:51:36.77 AS.seek 0.300ml @14:51:45.47 Exhaust.select :close @14:51:45.57 SSV.dial CBV @14:51:45.59 CBV.dial SSV @14:51:46.34 Allowing 185s for 25.0ml of remaining 550.0ml @14:51:46.36 Intake.select :open @14:51:48.49 Intake @33.2psia, vacuum from 20.0 to 24.0psi @14:51:48.51 SS.reconfigure forSampling @14:51:48.54 SS.seek 25.00ml @14:52:02.79 Stroke completed -- waiting for pressure to equalize ... @14:52:03.43 Intake.select :close @14:52:03.57 SSV.dial :exhaust @14:52:04.31 Exhaust.select :open @14:52:04.41 SS.reconfigure SSexhale @14:52:04.45 SS.seek 0.00ml @14:52:13.20 Exhaust.select :close @14:52:13.30 SSV.dial CBV @14:52:13.32 CBV.dial SSV @14:52:14.08 Allowing 185s for 25.0ml of remaining 525.0ml @14:52:14.09 Intake.select :open @14:52:16.23 Intake @33.2psia, vacuum from 20.0 to 24.0psi @14:52:16.25 SS.reconfigure forSampling @14:52:16.28 SS.seek 25.00ml @14:52:21.91 delay 20 seconds @14:52:30.40 Stroke completed -- waiting for pressure to equalize ... @14:52:31.04 Intake.select :close @14:52:31.17 SSV.dial :exhaust @14:52:31.92 Exhaust.select :open @14:52:32.03 SS.reconfigure SSexhale @14:52:32.07 SS.seek 0.00ml @14:52:40.64 Exhaust.select :close @14:52:40.74 SSV.dial CBV @14:52:40.76 CBV.dial SSV @14:52:41.52 Allowing 185s for 25.0ml of remaining 500.0ml @14:52:41.53 Intake.select :open @14:52:41.92 AS.seek 0.070ml @14:52:43.66 Intake @33.2psia, vacuum from 20.0 to 24.0psi @14:52:43.68 SS.reconfigure forSampling @14:52:43.71 SS.seek 25.00ml @14:52:57.93 Stroke completed -- waiting for pressure to equalize ... @14:52:58.57 Intake.select :close @14:52:58.70 SSV.dial :exhaust @14:52:59.45 Exhaust.select :open @14:52:59.55 SS.reconfigure SSexhale @14:52:59.59 SS.seek 0.00ml @14:53:08.18 Exhaust.select :close @14:53:08.29 SSV.dial CBV @14:53:08.31 CBV.dial SSV @14:53:09.06 Allowing 185s for 25.0ml of remaining 475.0ml @14:53:09.07 Intake.select :open @14:53:11.21 Intake @33.1psia, vacuum from 20.0 to 24.0psi @14:53:11.23 SS.reconfigure forSampling @14:53:11.26 SS.seek 25.00ml @14:53:25.38 Stroke completed -- waiting for pressure to equalize ... @14:53:26.02 Intake.select :close @14:53:26.17 SSV.dial :exhaust @14:53:26.92 Exhaust.select :open @14:53:27.03 SS.reconfigure SSexhale @14:53:27.07 SS.seek 0.00ml @14:53:29.08 delay 20 seconds @14:53:36.12 Exhaust.select :close @14:53:36.22 SSV.dial CBV @14:53:36.24 CBV.dial SSV @14:53:36.99 Allowing 185s for 25.0ml of remaining 450.0ml @14:53:37.00 Intake.select :open @14:53:39.14 Intake @33.2psia, vacuum from 20.0 to 24.0psi @14:53:39.16 SS.reconfigure forSampling @14:53:39.19 SS.seek 25.00ml @14:53:49.09 ATV.dial :waste2 @14:53:49.73 AS.reconfigure ASconfig @14:53:49.76 AS.seek empty @14:53:53.37 Stroke completed -- waiting for pressure to equalize ... @14:53:54.02 Intake.select :close @14:53:54.17 SSV.dial :exhaust @14:53:54.22 Elution ATV.dial :air1 @14:53:54.94 Exhaust.select :open @14:53:55.03 SS.reconfigure SSexhale @14:53:55.07 SS.seek 0.00ml @14:53:55.18 AS.reconfigure ASslow1 @14:53:55.21 AS.seek 0.049ml @14:54:03.65 Exhaust.select :close @14:54:03.76 SSV.dial CBV @14:54:03.78 CBV.dial SSV @14:54:04.55 Allowing 185s for 25.0ml of remaining 425.0ml @14:54:04.57 Intake.select :open @14:54:05.70 delay 3 seconds @14:54:06.70 Intake @33.2psia, vacuum from 20.0 to 24.0psi @14:54:06.72 SS.reconfigure forSampling @14:54:06.75 SS.seek 25.00ml @14:54:08.71 ATV.dial :ElutionWater @14:54:09.35 AS.seek 0.109ml @14:54:20.90 Stroke completed -- waiting for pressure to equalize ... @14:54:21.54 Intake.select :close @14:54:21.69 SSV.dial :exhaust @14:54:21.86 delay 3 seconds @14:54:22.44 Exhaust.select :open @14:54:22.54 SS.reconfigure SSexhale @14:54:22.58 SS.seek 0.00ml @14:54:24.87 ATV.dial ASV,via => air2 @14:54:26.50 ASV.dial :SPEelution @14:54:27.36 AFV.select :carrierSolution @14:54:27.51 AS.reconfigure ASnorm @14:54:27.54 AS.seek 0.759ml @14:54:31.22 Exhaust.select :close @14:54:31.33 SSV.dial CBV @14:54:31.35 CBV.dial SSV @14:54:32.11 Allowing 185s for 25.0ml of remaining 400.0ml @14:54:32.13 Intake.select :open @14:54:34.26 Intake @33.2psia, vacuum from 20.0 to 24.0psi @14:54:34.28 SS.reconfigure forSampling @14:54:34.31 SS.seek 25.00ml @14:54:48.46 Stroke completed -- waiting for pressure to equalize ... @14:54:49.10 Intake.select :close @14:54:49.25 SSV.dial :exhaust @14:54:50.00 Exhaust.select :open @14:54:50.11 SS.reconfigure SSexhale @14:54:50.15 SS.seek 0.00ml @14:54:58.94 Exhaust.select :close @14:54:59.04 SSV.dial CBV @14:54:59.06 CBV.dial SSV @14:54:59.83 Allowing 185s for 25.0ml of remaining 375.0ml @14:54:59.84 Intake.select :open @14:55:01.03 delay 3 seconds @14:55:01.98 Intake @33.2psia, vacuum from 20.0 to 24.0psi @14:55:02.00 SS.reconfigure forSampling @14:55:02.03 SS.seek 25.00ml @14:55:04.04 AFV.select :holdingCoil @14:55:04.19 AS.reconfigure ASslow1 @14:55:04.22 AS.seek 0.489ml @14:55:16.32 Stroke completed -- waiting for pressure to equalize ... @14:55:16.96 Intake.select :close @14:55:17.09 SSV.dial :exhaust @14:55:17.85 Exhaust.select :open @14:55:17.95 SS.reconfigure SSexhale @14:55:17.99 SS.seek 0.00ml @14:55:26.66 Exhaust.select :close @14:55:26.76 SSV.dial CBV @14:55:26.78 CBV.dial SSV @14:55:27.55 Allowing 185s for 25.0ml of remaining 350.0ml @14:55:27.56 Intake.select :open @14:55:29.70 Intake @33.1psia, vacuum from 20.0 to 24.0psi @14:55:29.72 SS.reconfigure forSampling @14:55:29.75 SS.seek 25.00ml @14:55:44.00 Stroke completed -- waiting for pressure to equalize ... @14:55:44.63 Intake.select :close @14:55:44.78 SSV.dial :exhaust @14:55:45.53 Exhaust.select :open @14:55:45.63 SS.reconfigure SSexhale @14:55:45.67 SS.seek 0.00ml @14:55:54.36 Exhaust.select :close @14:55:54.47 SSV.dial CBV @14:55:54.49 CBV.dial SSV @14:55:55.25 Allowing 185s for 25.0ml of remaining 325.0ml @14:55:55.26 Intake.select :open @14:55:57.40 Intake @33.3psia, vacuum from 20.0 to 24.0psi @14:55:57.42 SS.reconfigure forSampling @14:55:57.45 SS.seek 25.00ml @14:55:59.53 delay 5 seconds @14:56:04.59 AS.reconfigure ASslow2 @14:56:04.62 AS.seek 0.454ml @14:56:11.23 Stroke completed -- waiting for pressure to equalize ... @14:56:11.87 Intake.select :close @14:56:12.00 SSV.dial :exhaust @14:56:12.75 Exhaust.select :open @14:56:12.85 SS.reconfigure SSexhale @14:56:12.89 SS.seek 0.00ml @14:56:21.80 Exhaust.select :close @14:56:21.91 SSV.dial CBV @14:56:21.93 CBV.dial SSV @14:56:22.70 Allowing 185s for 25.0ml of remaining 300.0ml @14:56:22.71 Intake.select :open @14:56:25.28 Intake @33.2psia, vacuum from 20.0 to 24.0psi @14:56:25.30 SS.reconfigure forSampling @14:56:25.33 SS.seek 25.00ml @14:56:39.34 Stroke completed -- waiting for pressure to equalize ... @14:56:39.98 Intake.select :close @14:56:40.12 SSV.dial :exhaust @14:56:40.74 delay 1:00 @14:56:40.87 Exhaust.select :open @14:56:40.97 SS.reconfigure SSexhale @14:56:41.01 SS.seek 0.00ml @14:56:49.48 Exhaust.select :close @14:56:49.58 SSV.dial CBV @14:56:49.60 CBV.dial SSV @14:56:50.36 Allowing 185s for 25.0ml of remaining 275.0ml @14:56:50.37 Intake.select :open @14:56:52.51 Intake @33.2psia, vacuum from 20.0 to 24.0psi @14:56:52.53 SS.reconfigure forSampling @14:56:52.56 SS.seek 25.00ml @14:57:06.34 Stroke completed -- waiting for pressure to equalize ... @14:57:06.98 Intake.select :close @14:57:07.12 SSV.dial :exhaust @14:57:07.86 Exhaust.select :open @14:57:07.96 SS.reconfigure SSexhale @14:57:08.00 SS.seek 0.00ml @14:57:16.75 Exhaust.select :close @14:57:16.85 SSV.dial CBV @14:57:16.87 CBV.dial SSV @14:57:17.62 Allowing 185s for 25.0ml of remaining 250.0ml @14:57:17.63 Intake.select :open @14:57:19.77 Intake @33.3psia, vacuum from 20.0 to 24.0psi @14:57:19.79 SS.reconfigure forSampling @14:57:19.82 SS.seek 25.00ml @14:57:34.07 Stroke completed -- waiting for pressure to equalize ... @14:57:34.71 Intake.select :close @14:57:34.85 SSV.dial :exhaust @14:57:35.59 Exhaust.select :open @14:57:35.69 SS.reconfigure SSexhale @14:57:35.73 SS.seek 0.00ml @14:57:40.75 pushing eluate to holding coil @14:57:40.81 AS.seek 0.294ml @14:57:44.53 Exhaust.select :close @14:57:44.63 SSV.dial CBV @14:57:44.65 CBV.dial SSV @14:57:45.40 Allowing 185s for 25.0ml of remaining 225.0ml @14:57:45.41 Intake.select :open @14:57:47.60 Intake @33.2psia, vacuum from 20.0 to 24.0psi @14:57:47.61 SS.reconfigure forSampling @14:57:47.69 SS.seek 25.00ml @14:57:48.02 Session Begun @14:57:57.43 -> showlog @14:58:01.49 Stroke completed -- waiting for pressure to equalize ... @14:58:02.13 Intake.select :close @14:58:02.28 SSV.dial :exhaust @14:58:03.04 Exhaust.select :open @14:58:03.15 SS.reconfigure SSexhale @14:58:03.20 SS.seek 0.00ml @14:58:11.77 Exhaust.select :close @14:58:11.87 SSV.dial CBV @14:58:11.89 CBV.dial SSV @14:58:12.67 Allowing 185s for 25.0ml of remaining 200.0ml @14:58:12.69 Intake.select :open @14:58:14.83 Intake @33.4psia, vacuum from 20.0 to 24.0psi @14:58:14.85 SS.reconfigure forSampling @14:58:14.91 SS.seek 25.00ml @14:58:29.35 Stroke completed -- waiting for pressure to equalize ... @14:58:29.99 Intake.select :close @14:58:30.14 SSV.dial :exhaust @14:58:30.90 Exhaust.select :open @14:58:31.00 SS.reconfigure SSexhale @14:58:31.05 SS.seek 0.00ml @14:58:39.79 Exhaust.select :close @14:58:39.89 SSV.dial CBV @14:58:39.91 CBV.dial SSV @14:58:40.68 Allowing 185s for 25.0ml of remaining 175.0ml @14:58:40.70 Intake.select :open @14:58:42.86 Intake @33.3psia, vacuum from 20.0 to 24.0psi @14:58:42.88 SS.reconfigure forSampling @14:58:42.94 SS.seek 25.00ml @14:58:57.05 Stroke completed -- waiting for pressure to equalize ... @14:58:57.69 Intake.select :close @14:58:57.83 SSV.dial :exhaust @14:58:58.58 Exhaust.select :open @14:58:58.69 SS.reconfigure SSexhale @14:58:58.74 SS.seek 0.00ml @14:59:07.43 Exhaust.select :close @14:59:07.53 SSV.dial CBV @14:59:07.55 CBV.dial SSV @14:59:08.32 Allowing 185s for 25.0ml of remaining 150.0ml @14:59:08.34 Intake.select :open @14:59:10.49 Intake @33.2psia, vacuum from 20.0 to 24.0psi @14:59:10.51 SS.reconfigure forSampling @14:59:10.57 SS.seek 25.00ml @14:59:24.69 Stroke completed -- waiting for pressure to equalize ... @14:59:25.33 Intake.select :close @14:59:25.47 SSV.dial :exhaust @14:59:26.22 Exhaust.select :open @14:59:26.33 SS.reconfigure SSexhale @14:59:26.38 SS.seek 0.00ml @14:59:34.89 Exhaust.select :close @14:59:34.99 SSV.dial CBV @14:59:35.01 CBV.dial SSV @14:59:35.77 Allowing 185s for 25.0ml of remaining 125.0ml @14:59:36.30 Intake.select :open @14:59:38.46 Intake @33.5psia, vacuum from 20.0 to 24.0psi @14:59:38.48 SS.reconfigure forSampling @14:59:38.54 SS.seek 25.00ml @14:59:53.00 Stroke completed -- waiting for pressure to equalize ... @14:59:53.64 Intake.select :close @14:59:53.78 SSV.dial :exhaust @14:59:54.53 Exhaust.select :open @14:59:54.64 SS.reconfigure SSexhale @14:59:54.69 SS.seek 0.00ml @15:00:03.42 Exhaust.select :close @15:00:03.52 SSV.dial CBV @15:00:03.54 CBV.dial SSV @15:00:04.32 Allowing 185s for 25.0ml of remaining 100.0ml @15:00:04.34 Intake.select :open @15:00:06.49 Intake @33.3psia, vacuum from 20.0 to 24.0psi @15:00:06.51 SS.reconfigure forSampling @15:00:06.57 SS.seek 25.00ml @15:00:20.77 Stroke completed -- waiting for pressure to equalize ... @15:00:21.41 Intake.select :close @15:00:21.55 SSV.dial :exhaust @15:00:22.31 Exhaust.select :open @15:00:22.42 SS.reconfigure SSexhale @15:00:22.47 SS.seek 0.00ml @15:00:24.24 delay 30 seconds @15:00:31.11 Exhaust.select :close @15:00:31.22 SSV.dial CBV @15:00:31.24 CBV.dial SSV @15:00:32.00 Allowing 185s for 25.0ml of remaining 75.0ml @15:00:32.02 Intake.select :open @15:00:34.17 Intake @33.4psia, vacuum from 20.0 to 24.0psi @15:00:34.19 SS.reconfigure forSampling @15:00:34.25 SS.seek 25.00ml @15:00:48.36 Stroke completed -- waiting for pressure to equalize ... @15:00:49.01 Intake.select :close @15:00:49.17 SSV.dial :exhaust @15:00:49.94 Exhaust.select :open @15:00:50.05 SS.reconfigure SSexhale @15:00:50.10 SS.seek 0.00ml @15:00:54.25 ATV.dial :waste,via => air3 @15:00:55.70 AS.reconfigure ASconfig @15:00:55.74 AS.seek empty @15:00:58.59 Exhaust.select :close @15:00:58.69 SSV.dial CBV @15:00:58.71 CBV.dial SSV @15:00:59.50 Allowing 185s for 25.0ml of remaining 50.0ml @15:00:59.52 Intake.select :open @15:01:01.66 Intake @33.4psia, vacuum from 20.0 to 24.0psi @15:01:01.68 SS.reconfigure forSampling @15:01:01.74 SS.seek 25.00ml @15:01:13.24 Parking Bleach in LysateSource line and SPEcolumn @15:01:13.25 ATV.dial :air3 @15:01:13.91 AS.reconfigure ASslow1 @15:01:13.95 AS.seek 0.029ml @15:01:15.98 Stroke completed -- waiting for pressure to equalize ... @15:01:16.62 Intake.select :close @15:01:16.77 SSV.dial :exhaust @15:01:17.55 Exhaust.select :open @15:01:17.66 SS.reconfigure SSexhale @15:01:17.71 SS.seek 0.00ml @15:01:20.39 ATV.dial :ElutionWater @15:01:20.78 AS.seek 0.059ml @15:01:26.62 Exhaust.select :close @15:01:26.72 SSV.dial CBV @15:01:26.74 CBV.dial SSV @15:01:27.17 ATV.dial :air3 @15:01:27.58 Allowing 185s for 25.0ml of remaining 25.0ml @15:01:27.59 Intake.select :open @15:01:27.65 AS.seek 0.069ml @15:01:29.74 Intake @33.3psia, vacuum from 20.0 to 24.0psi @15:01:29.76 SS.reconfigure forSampling @15:01:29.82 SS.seek 24.98ml @15:01:29.95 delay 2 seconds @15:01:31.96 ATV.dial :bleach @15:01:32.35 AS.seek 0.709ml @15:01:43.92 Stroke completed -- waiting for pressure to equalize ... @15:01:44.56 Intake.select :close @15:01:44.70 SSV.dial :exhaust @15:01:45.47 Exhaust.select :open @15:01:45.59 SS.reconfigure SSexhale @15:01:45.64 SS.seek 0.00ml @15:01:54.14 Exhaust.select :close @15:01:54.24 Sampled 1000.0ml @15:01:54.25 collectSample: Complete @15:01:54.27 Drying puck before fixing for 04:00.00 @15:01:54.29 CBV.dial :waste2b @15:01:55.04 delay 1:00 @15:02:55.04 CSV.dial :air @15:02:55.49 CS.reconfigure CSfast @15:02:55.54 CS.seek 10.000ml @15:03:19.06 CBV.dial between CSV and waste2b @15:03:19.39 Pumping wcr puck from tube 2 up to 55psi... @15:03:19.45 CBV.dial SSV @15:03:20.88 CBV.dial between CSV and waste2b @15:03:21.82 CSV.dial between air and CTV @15:03:22.22 CS.seek 7.188ml @15:03:29.38 CTV.dial CSV,avoiding => air @15:03:29.90 CSV.dial CTV @15:03:30.66 CS.reconfigure CSpressurize @15:03:30.71 CS.seek 0.000ml @15:03:43.15 delay 3 seconds @15:03:46.16 ATV.dial :air3 @15:03:46.51 delay 3 seconds @15:03:49.46 CSV.dial :air @15:03:49.57 AS.seek 0.734ml @15:03:49.99 CS.reconfigure CSfast @15:03:50.04 CS.seek 10.000ml @15:03:54.93 delay 3 seconds @15:03:57.94 ATV.dial :bleach @15:03:58.43 AS.seek 0.719ml @15:04:01.75 delay 3 seconds @15:04:04.76 ATV.dial :AIV @15:04:05.86 AS.reconfigure ASconfig @15:04:05.90 AS.seek 0.015ml @15:04:13.50 CSV.dial between air and CTV @15:04:14.52 CS.seek 2.365ml @15:04:32.62 CTV.dial CSV,avoiding => air @15:04:32.63 CSV.dial CTV @15:04:33.05 CS.reconfigure CSpressurize @15:04:33.10 CS.seek 0.000ml @15:04:35.73 CBV.dial :waste2b @15:04:36.06 CS.reconfigure CSconfig @15:04:36.11 CS.seek empty @15:04:40.68 delay 1:00 @15:04:47.24 ATV.dial :waste @15:04:48.51 AS.seek empty @15:04:49.74 ATV.dial :air3 @15:04:50.40 AS.reconfigure ASslow1 @15:04:50.45 AS.seek 0.009ml @15:04:52.80 delay 2 seconds @15:04:54.81 ATV.dial :bleach @15:04:55.19 AS.seek 0.579ml @15:05:40.69 CTV.dial :air,avoiding => intake @15:05:42.00 Fix #1 of 2 (with rnal) for 02:00.00 @15:05:42.01 CS Pulling 2ml of rnal @15:05:42.02 CSV.dial CSR @15:05:42.47 CSR.select :rnal @15:05:42.59 CS.seek 2.000ml @15:05:48.52 delay 5 seconds @15:05:53.53 CSR.select :air @15:05:53.63 CS.reconfigure CSfast @15:05:53.68 CS.seek 10.000ml @15:06:12.52 CBV.dial between CSV and waste2b @15:06:12.87 Pumping wcr puck from tube 2 up to 55psi... @15:06:12.93 CBV.dial SSV @15:06:14.35 CBV.dial between CSV and waste2b @15:06:15.30 CSV.dial between air and CTV @15:06:15.98 CS.seek 6.994ml @15:06:23.62 CTV.dial CSV,avoiding => air @15:06:23.64 CSV.dial CTV @15:06:24.96 CS.reconfigure CSpressurize @15:06:25.01 CS.seek 0.000ml @15:06:42.94 delay 2:00 @15:06:51.69 delay 3 seconds @15:06:54.70 ATV.dial :air3 @15:06:55.08 delay 3 seconds @15:06:58.14 AS.seek 0.604ml @15:07:03.50 delay 3 seconds @15:07:06.51 ATV.dial :bleach @15:07:06.89 AS.seek 0.589ml @15:07:10.22 delay 3 seconds @15:07:13.23 ATV.dial :ASV @15:07:14.22 ASV.dial :wasteR @15:07:14.79 AS.seek 0.000ml @15:08:42.95 CBV.dial :waste2b @15:08:43.28 CS.reconfigure CSconfig @15:08:43.32 CS.seek empty @15:08:44.13 delay 1:00 @15:09:15.24 delay 1:00 @15:09:44.14 CTV.dial :air,avoiding => intake @15:09:45.44 Fix #2 of 2 (with rnal) for 02:00.00 @15:09:45.45 CS Pulling 2ml of rnal @15:09:45.46 CSV.dial CSR @15:09:45.92 CSR.select :rnal @15:09:46.02 CS.seek 2.000ml @15:09:51.94 delay 5 seconds @15:09:56.95 CSR.select :air @15:09:57.06 CS.reconfigure CSfast @15:09:57.11 CS.seek 10.000ml @15:10:15.25 AFV.select :carrierSolution @15:10:15.35 delay 2 seconds @15:10:15.97 CBV.dial between CSV and waste2b @15:10:16.31 Pumping wcr puck from tube 2 up to 55psi... @15:10:16.37 CBV.dial SSV @15:10:17.36 AS.reconfigure ASconfig @15:10:17.41 AS.seek 1.000ml @15:10:17.79 CBV.dial between CSV and waste2b @15:10:18.74 CSV.dial between air and CTV @15:10:19.40 CS.seek 6.858ml @15:10:27.45 CTV.dial CSV,avoiding => air @15:10:27.95 CSV.dial CTV @15:10:29.33 CS.reconfigure CSpressurize @15:10:29.38 CS.seek 0.000ml @15:10:46.87 delay 2:00 @15:11:16.05 delay 1 second @15:11:17.07 AFV.select :holdingCoil @15:11:17.17 delay 1 second @15:11:18.18 ATV.dial :ASV @15:11:18.19 ASV.dial :wasteR @15:11:18.21 AS.reconfigure ASslow1 @15:11:18.26 AS.seek 0.000ml @15:12:46.88 CBV.dial :waste2b @15:12:47.21 CS.reconfigure CSconfig @15:12:47.26 CS.seek empty @15:12:48.40 delay 1:00 @15:13:48.41 CTV.dial :air,avoiding => intake @15:13:49.73 Storing wcr puck in Collection Clamp to tube 1 @15:13:49.88 SC.reconfigure SCtight @15:13:49.93 SC.seek 1 @15:13:52.18 SC.reconfigure SCloose @15:13:52.37 Elbow.reconfigure TightElbow @15:13:52.42 Elbow.seek 1 @15:13:54.02 Forearm.reconfigure TightForearm @15:13:54.07 Forearm.seek 1 + 800 counts @15:13:55.24 SE.reconfigure SEpick @15:13:55.31 SE.seek beamBreak @15:13:58.21 SE.configure SEconfig @15:13:58.35 Elbow.reconfigure LimpElbow @15:13:58.40 Forearm.reconfigure LooseForearm @15:13:58.45 Forearm.seek 1 @15:13:58.71 Elbow.hold @15:13:58.77 Elbow.reconfigure TightElbow @15:13:58.87 SE.seek 1.05 Pucks below Top Plate @15:13:59.20 Forearm.coast @15:13:59.25 Forearm.seek 1 + 348 counts @15:13:59.53 Forearm.reconfigure TightForearm @15:13:59.58 Forearm.seek 1 + 800 counts @15:13:59.86 SE.seek 2.05 Pucks below Top Plate @15:14:00.43 Forearm.reconfigure LooseForearm @15:14:00.48 Forearm.seek retract @15:14:01.46 Elbow.seek CC @15:14:02.98 Elbow.reconfigure LooseElbow @15:14:03.07 Forearm.seek CC @15:14:04.03 CC.seek sealed @15:14:04.99 Hand.grip @15:14:05.77 CC.seek open @15:14:09.14 Forearm.seek retract @15:14:10.11 Elbow.reconfigure TightElbow @15:14:10.16 Elbow.seek 1 @15:14:11.75 Elbow.reconfigure LimpElbow @15:14:11.81 Forearm.seek 1 @15:14:12.84 Elbow.hold @15:14:12.91 Elbow.reconfigure TightElbow @15:14:12.96 Hand.release @15:14:13.72 Forearm.coast @15:14:13.77 Forearm.seek 1 + 329 counts @15:14:14.04 Forearm.reconfigure TightForearm @15:14:14.09 Forearm.seek 1 + 800 counts @15:14:14.43 SE.seek down @15:14:15.53 Forearm.reconfigure LooseForearm @15:14:15.58 Forearm.seek retract @15:14:16.95 SE.coast @15:14:16.99 SC.coast @15:14:17.03 Starting finalPurge @15:14:17.04 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:14:17.79 Elbow.seek FlushPuck::Garage @15:14:19.60 Elbow.reconfigure LooseElbow @15:14:19.69 Forearm.seek garage @15:14:20.75 Hand.grip @15:14:21.61 Forearm.seek retract @15:14:22.66 Elbow.reconfigure TightElbow @15:14:22.70 Elbow.seek CC @15:14:25.48 Elbow.reconfigure LooseElbow @15:14:25.57 Forearm.reconfigure TightForearm @15:14:25.62 Forearm.seek CC @15:14:26.73 CC.seek closed @15:14:30.51 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 2.07 @15:14:30.58 Hand.release @15:14:31.31 Forearm.reconfigure LooseForearm @15:14:31.36 Forearm.seek retract @15:14:32.30 Flushing the Collection Valve::Manifold @15:14:32.43 CS Pulling 5ml of flush CSV.dial CSR @15:14:33.35 CSR.select :flush @15:14:33.47 CS.seek 5.000ml @15:14:42.48 delay 20 seconds @15:14:46.96
delay 5 seconds @15:14:51.97 CSR.select :air @15:14:52.13 CS.reconfigure CSfast @15:14:52.17 CS.seek 7.500ml @15:14:58.71 CSV.dial :waste @15:14:59.98 CS.reconfigure CSconfig @15:15:00.02 CS.seek 0.000ml @15:15:02.49 ATV.dial :air3 @15:15:03.61 AS.seek 0.590ml @15:15:19.83
Flushing lower manifold's waste1 port @15:15:19.94 CSV.dial :flush @15:15:20.41 CS.reconfigure CSfast @15:15:20.45 CS.seek 5.000ml @15:15:32.52 delay 5 seconds @15:15:37.53 CSV.dial :air @15:15:38.02 CS.seek 6.999ml @15:15:43.40 CSV.dial CTV @15:15:43.43
CTV.dial CSV @15:15:44.75 CBV.dial :waste1 @15:15:45.51 CS.seek 0.000ml @15:16:02.02 Flushing lower manifold's waste2b port @15:16:02.14 CSV.dial :flush @15:16:02.95 CS.seek 5.000ml @15:16:15.05 delay 5 seconds @15:16:20.06 CSV.dial :air @15:16:20.63 CS.seek 7.000ml @15:16:26.14 CSV.dial CTV @15:16:26.18
CTV.dial CSV @15:16:26.64 CBV.dial :waste2b @15:16:27.39 CS.seek 0.000ml @15:16:43.95 Flushing upper manifold @15:16:44.07 CSV.dial :flush @15:16:44.89 CS.seek 5.000ml @15:16:57.02 delay 5 seconds @15:17:02.03 CSV.dial :air @15:17:03.00 CS.seek 7.000ml @15:17:04.17 delay 3 seconds @15:17:07.17 ATV.dial :ASV @15:17:08.26 ASV.dial :wasteR @15:17:08.34 AS.seek 0.090ml @15:17:08.45 CSV.dial CBV @15:17:08.48
CBV.dial CSV @15:17:10.01 CTV.dial :air @15:17:11.32 CS.seek 0.000ml @15:17:27.95 delay 7 seconds @15:17:34.96 CS.seek 3.000ml @15:17:42.58 CSV.dial :waste @15:17:43.10 CS.seek 0.000ml @15:17:51.23 CSV.dial :flush @15:17:51.68 CS.seek 3.000ml @15:17:59.26 delay 5 seconds @15:18:04.27 CSV.dial :air @15:18:04.75 CS.seek 4.999ml @15:18:10.11 CSV.dial :air @15:18:10.13 CS.seek 0.000ml @15:18:22.11 delay 7 seconds @15:18:29.12 CS.seek 3.000ml @15:18:36.76 CSV.dial :waste @15:18:37.57 CS.seek 0.000ml @15:18:45.20 Disinfecting external Intake with mfbkill @15:18:45.32 CSV.dial CSR @15:18:46.67 CSR.select :mfbkill @15:18:46.78 CS.seek 8.000ml @15:18:50.58 delay 30 seconds @15:19:05.60
delay 5 seconds @15:19:10.61 CSR.select :air @15:19:10.72 CS.seek 10.000ml @15:19:16.08 CSV.dial CBV @15:19:16.10
CBV.dial CSV @15:19:17.08 CTV.dial :intake @15:19:17.82 Intake.select :open @15:19:17.93 CS.seek 2.000ml @15:19:20.59 ATV.dial :extSrc @15:19:21.11 delay 3 seconds @15:19:24.12 ATV.dial :ElutionWater @15:19:25.05 AS.seek 0.120ml @15:19:31.43 ATV.dial :air3 @15:19:31.82 AS.seek 0.130ml @15:19:34.13 delay 2 seconds @15:19:36.14 ATV.dial :bleach @15:19:36.52 AS.seek 0.230ml @15:19:37.22
Intake.select :close @15:19:37.37 CTV.dial :air @15:19:38.11 CS.reconfigure CSconfig @15:19:38.16 CS.seek 0.000ml @15:19:44.23 Flushing lower manifold's waste1 port @15:19:44.24 CS Pulling 4ml of mfbkill @15:19:44.26 CSV.dial CSR @15:19:45.32 CSR.select :mfbkill @15:19:45.43 CS.seek 4.000ml @15:19:56.41 delay 5 seconds @15:19:57.10 delay 3 seconds @15:20:00.11 ATV.dial :air3 @15:20:00.54 delay 3 seconds @15:20:01.43
CSR.select :air @15:20:01.54 CS.reconfigure CSfast @15:20:01.59 CS.seek 10.000ml @15:20:03.60 AS.seek 0.255ml @15:20:09.00 delay 3 seconds @15:20:12.01 ATV.dial :bleach @15:20:12.50 AS.seek 0.240ml @15:20:15.82 delay 3 seconds @15:20:16.00 CSV.dial CTV @15:20:16.02
CTV.dial CSV @15:20:17.37 CBV.dial :waste1 @15:20:17.82 CS.seek 0.000ml @15:20:18.83 ATV.dial :ASV @15:20:19.82 ASV.dial :SPEdelivery @15:20:20.31 AFV.select :carrierSolution @15:20:20.41 delay 2 seconds @15:20:22.42 AS.reconfigure ASconfig @15:20:22.47 AS.seek 0.500ml @15:20:37.92 delay 1 second @15:20:38.93 AFV.select :holdingCoil @15:20:39.08 AS.reconfigure ASslow1 @15:20:39.12 AS.seek 0.170ml @15:20:41.17
Flushing lower manifold's waste2b port @15:20:41.19 CS Pulling 4ml of mfbkill @15:20:41.20 CSV.dial CSR @15:20:41.72 CSR.select :mfbkill @15:20:41.83 CS.reconfigure CSconfig @15:20:41.88 CS.seek 4.000ml @15:20:52.83 delay 5 seconds @15:20:57.84 CSR.select :air @15:20:57.95 CS.reconfigure CSfast @15:20:58.00 CS.seek 10.000ml @15:21:12.31 CSV.dial CTV @15:21:12.33
CTV.dial CSV @15:21:12.85 CBV.dial :waste2b @15:21:13.60 CS.seek 0.000ml @15:21:36.70 Killing upper manifold @15:21:36.71 CS Pulling 4ml of mfbkill @15:21:36.72 CSV.dial CSR @15:21:37.25 CSR.select :mfbkill @15:21:37.36 CS.reconfigure CSconfig @15:21:37.41 CS.seek 4.000ml @15:21:46.69 SPE.hold 2:46:40,95.0C @15:21:46.71 delay 3:00 @15:21:48.39
delay 5 seconds @15:21:53.40 CSR.select :air @15:21:53.58 CS.reconfigure CSfast @15:21:53.67 CS.seek 10.000ml @15:22:08.54 CSV.dial CBV @15:22:08.56
CBV.dial CSV @15:22:09.64 CTV.dial :air @15:22:10.97 CS.seek 0.000ml @15:22:35.07 delay 7 seconds @15:22:42.08 CS.seek 3.000ml @15:22:49.95 CSV.dial :waste @15:22:50.41 CS.seek 0.000ml @15:22:58.29 Flushing upper manifold @15:22:58.92 CSV.dial :flush @15:22:59.38 CS.seek 5.000ml @15:23:11.95 delay 5 seconds @15:23:16.96 CSV.dial :air @15:23:17.47 CS.seek 6.999ml @15:23:23.10 CSV.dial CBV @15:23:23.13
CBV.dial CSV @15:23:24.25 CTV.dial :air @15:23:24.26 CS.seek 0.000ml @15:23:41.66 delay 7 seconds @15:23:48.67 CS.seek 3.000ml @15:23:56.66 CSV.dial :waste @15:23:57.20 CS.seek 0.000ml @15:24:05.13 CSV.dial :flush @15:24:05.59 CS.seek 3.000ml @15:24:13.44 delay 5 seconds @15:24:18.45 CSV.dial :air @15:24:18.95 CS.seek 5.000ml @15:24:24.44 CSV.dial :air @15:24:24.46 CS.seek 0.000ml @15:24:26.52 Session Begun @15:24:37.20
delay 7 seconds @15:24:44.20 CS.seek 3.000ml @15:24:46.72 AFV.select :carrierSolution @15:24:46.82 delay 2 seconds @15:24:48.83 AS.reconfigure ASconfig @15:24:48.88 AS.seek 1.000ml @15:24:52.04
CSV.dial :waste @15:24:52.87 CS.seek 0.000ml @15:25:00.69 Flushing lower manifold's waste1 port @15:25:00.82 CSV.dial :flush @15:25:01.37 CS.seek 5.000ml @15:25:13.72 delay 5 seconds @15:25:18.73 CSV.dial :air @15:25:19.44 CS.seek 7.000ml @15:25:24.91 CSV.dial CTV @15:25:24.94
CTV.dial CSV @15:25:26.30 CBV.dial :waste1 @15:25:26.79 CS.seek 0.000ml @15:25:37.55 delay 1 second @15:25:38.56 AFV.select :holdingCoil @15:25:38.68 delay 1 second @15:25:39.69 ASV.dial :wasteD @15:25:40.71 AS.seek 0.000ml @15:25:43.70
Flushing lower manifold's waste2b port @15:25:43.82 CSV.dial :flush @15:25:44.65 CS.seek 5.000ml @15:25:56.94 delay 5 seconds @15:26:01.95 CSV.dial :air @15:26:02.53 CS.seek 7.000ml @15:26:07.97 CSV.dial CTV @15:26:08.00
CTV.dial CSV @15:26:08.45 CBV.dial :waste2b @15:26:09.23 CS.seek 0.000ml @15:26:26.12 Disinfecting external Intake with kill @15:26:26.24 CSV.dial CSR @15:26:26.70 CSR.select :kill @15:26:26.81 CS.seek 8.000ml @15:26:39.33 Priming Eluate @15:26:39.34 AFV.select :carrierSolution @15:26:39.46 delay 2 seconds @15:26:41.47 AS.seek 0.500ml @15:26:45.91
delay 5 seconds @15:26:50.92 CSR.select :air @15:26:51.03 CS.seek 10.000ml @15:26:56.40 CSV.dial CBV @15:26:56.43
CBV.dial CSV @15:26:57.47 CTV.dial :intake @15:26:58.22 Intake.select :open @15:26:58.33 CS.seek 2.000ml @15:27:10.90 delay 1 second @15:27:11.91 AFV.select :holdingCoil @15:27:12.01 ATV.dial ASV @15:27:12.02 ASV.dial :SPErecovery @15:27:12.70 Swish #1 of 2 @15:27:12.78 AS.reconfigure ASslow0 @15:27:12.83 AS.seek 0.600ml @15:27:17.97
Intake.select :close @15:27:18.08 CTV.dial :air @15:27:18.82 CS.reconfigure CSconfig @15:27:18.86 CS.seek 0.000ml @15:27:23.38 AS.seek 0.500ml @15:27:25.09
CSV.dial CSR @15:27:26.18 CSR.select :flush @15:27:26.30 CS.seek 5.000ml @15:27:33.92 Swish #2 of 2 @15:27:34.00 AS.seek 0.600ml @15:27:39.81
delay 5 seconds @15:27:44.56 AS.seek 0.500ml @15:27:44.82
CSR.select :air @15:27:44.93 CS.seek 10.000ml @15:27:55.09 delay 3 seconds @15:27:58.15 AS.reconfigure ASslow1 @15:27:58.20 AS.seek 0.676ml @15:27:58.46
CSV.dial CBV @15:27:59.55 CS.seek 0.000ml @15:28:26.28 Garaging FlushPuck in Collection Clamp @15:28:26.37 Elbow.reconfigure TightElbow @15:28:26.42 Elbow.seek CC @15:28:26.54 Elbow.reconfigure LooseElbow @15:28:26.64 Forearm.seek CC @15:28:27.63 CC.seek sealed @15:28:28.59 Hand.grip @15:28:29.36 CC.seek open @15:28:32.69 Forearm.seek retract @15:28:33.66 Elbow.reconfigure TightElbow @15:28:33.71 Elbow.seek garage @15:28:34.40 delay 3 seconds @15:28:36.53
Elbow.reconfigure LooseElbow @15:28:36.64 Forearm.reconfigure TightForearm @15:28:36.69 Forearm.seek garage @15:28:37.41 Eluate primed @15:28:37.42 ATV.dial :waste2 @15:28:37.85
Hand.release @15:28:38.07 AS.reconfigure ASnorm @15:28:38.12 AS.seek 0.000ml @15:28:38.65
Forearm.reconfigure LooseForearm @15:28:38.70 Forearm.seek retract @15:29:12.98 SPE.coast @15:29:13.08 ASV.dial :SPErecovery @15:29:13.10 Setting up PCR for IPCem -- writing /var/log/esp/IPCem14feb2615h1000ml.pcr @15:29:13.12 Building PCR Slug from ASV with MM3 mix, PP5 primer @15:29:13.13 Priming with carrier @15:29:13.15 ATV.dial ARV @15:29:13.87 ARV.dial :carrier @15:29:14.18 AS.seek 0.500ml @15:29:40.09 AFV.select :carrierSolution @15:29:40.20 delay 2 seconds @15:29:42.21 AS.reconfigure ASconfig @15:29:42.26 AS.seek 1.000ml @15:30:11.69 delay 1 second @15:30:12.70 AFV.select :holdingCoil @15:30:12.81 delay 1 second @15:30:13.82 ATV.dial :waste @15:30:15.08 AS.reconfigure ASnorm @15:30:15.14 AS.seek empty @15:31:06.65 AFV.select :carrierSolution @15:31:06.76 delay 2 seconds @15:31:08.77 AS.reconfigure ASconfig @15:31:08.83 AS.seek 0.500ml @15:31:38.33 delay 1 second @15:31:39.34 AFV.select :holdingCoil @15:31:39.45 Building Slug @15:31:39.46 ATV.dial :ARV @15:31:40.71 delay 2 seconds @15:31:42.72 ARV.dial :air1 @15:31:43.18 delay 2 seconds @15:31:45.24 AS.reconfigure ASslow2 @15:31:45.29 AS.seek 0.506ml @15:31:51.68 delay 2 seconds @15:31:53.69 ARV.dial :MM3 @15:31:54.55 delay 2 seconds @15:31:56.56 Adding MM3 @15:31:56.63 AS.seek 0.524ml @15:32:15.24 delay 25 seconds @15:32:40.25 ARV.dial :PP5 @15:32:40.61 Adding PP5 @15:32:40.62 delay 2 seconds @15:32:42.68 AS.seek 0.530ml @15:32:49.16 delay 10 seconds @15:32:59.17 ARV.dial :air3 @15:32:59.51 adding ARV air @15:32:59.52 delay 2 seconds @15:33:01.59 AS.seek 0.536ml @15:33:07.88 delay 5 seconds @15:33:12.89 ARV.dial :carrier @15:33:13.94 delay 7 seconds @15:33:21.00 AS.seek 0.594ml @15:34:20.53 delay 5 seconds @15:34:25.54 ATV.dial :ASV @15:34:25.89 delay 2 seconds @15:34:27.95 AS.seek 0.600ml @15:34:34.34 delay 4 seconds @15:34:38.35 ATV.dial ARV @15:34:38.70 delay 2 seconds @15:34:40.71 ARV.dial :carrier @15:34:40.72 delay 2 seconds @15:34:42.78 AS.seek 0.650ml @15:35:33.96 delay 4 seconds @15:35:37.97 ATV.dial PCR @15:35:38.50 ASV.dial :waste1 @15:35:39.20 ARV.dial :air1 @15:35:39.55 Positioning Slug by 0.324ml @15:35:39.63 AS.seek 0.326ml @15:41:08.83 -> Hi Blytheexit @15:41:08.84 NameError in ramu -- uninitialized constant Blytheexit @15:41:10.20 delay 5 seconds @15:41:15.21 ATV.dial ARV @15:41:15.74 ASV.dial :PCRlock @15:41:16.21 delay 5 seconds @15:41:17.71 -> exit @15:41:17.72 Session Ended @15:41:21.22 Running IPCem ProfileEM59C thermocycling @15:41:21.24 PCR.program ProfileEM59C @15:41:21.36 ProfileEM59C should complete in < 2:11:38 @15:41:21.37 PCR.run 2:49:32.5 @17:40:08.71 PCR.off @17:40:09.23 PCR for IPCem completed -- closed /var/log/esp/IPCem14feb2615h1000ml.pcr @17:40:09.30 Cleaning PCR @17:40:09.31 AFV.select :carrierSolution @17:40:09.41 delay 2 seconds @17:40:11.42 AS.reconfigure ASconfig @17:40:11.47 AS.seek 0.500ml @17:40:21.91 delay 1 second @17:40:22.92 AFV.select :holdingCoil @17:40:23.02 ASV.dial :waste1 @17:40:23.46 ATV.dial :air3 @17:40:24.41 AS.reconfigure ASslow1 @17:40:24.46 AS.seek 0.510ml @17:40:26.75 delay 2 seconds @17:40:28.76 ATV.dial :bleach @17:40:29.16 AS.seek 0.810ml @17:41:30.59 delay 3 seconds @17:41:33.60 ATV.dial :air3 @17:41:33.94 delay 3 seconds @17:41:37.00 AS.seek 0.835ml @17:41:42.36 delay 3 seconds @17:41:45.37 ATV.dial :bleach @17:41:45.86 AS.seek 0.820ml @17:41:49.17 delay 3 seconds @17:41:52.18 ARV.dial :waste @17:41:52.69 delay 3 seconds @17:41:55.70 ATV.dial :ARV @17:41:56.45 AS.reconfigure ASconfig @17:41:56.50 AS.seek 0.470ml @17:42:17.30 AFV.select :carrierSolution @17:42:17.40 delay 2 seconds @17:42:19.41 AS.seek 1.000ml @17:42:50.64 delay 1 second @17:42:51.65 AFV.select :holdingCoil @17:42:51.75 delay 1 second @17:42:52.76 AS.reconfigure ASfast @17:42:52.80 AS.seek 0.000ml @17:43:20.54 AFV.select :carrierSolution @17:43:20.65 delay 2 seconds @17:43:22.66 AS.reconfigure ASconfig @17:43:22.71 AS.seek 1.000ml @17:44:21.32 delay 1 second @17:44:22.33 AFV.select :holdingCoil @17:44:22.43 delay 1 second @17:44:23.44 AS.reconfigure ASfast @17:44:23.48 AS.seek 0.000ml @17:44:51.23 AFV.select :carrierSolution @17:44:51.34 delay 2 seconds @17:44:53.35 AS.reconfigure ASconfig @17:44:53.40 AS.seek 1.000ml @17:45:52.52 delay 1 second @17:45:53.53 AFV.select :holdingCoil @17:45:53.63 delay 1 second @17:45:54.64 AS.reconfigure ASfast @17:45:54.69 AS.seek 0.000ml @17:46:22.43 ARV.dial between carrier and air1 @17:46:22.96 ATV.dial :air3 @17:46:23.99 AS.reconfigure ASslow1 @17:46:24.04 AS.seek 0.010ml @17:46:26.36 delay 2 seconds @17:46:28.37 ATV.dial :bleach @17:46:28.76 AS.seek 0.810ml @17:49:12.19 delay 3 seconds @17:49:15.21 ATV.dial :air3 @17:49:15.55 delay 3 seconds @17:49:18.61 AS.seek 0.835ml @17:49:23.97 delay 3 seconds @17:49:26.98 ATV.dial :bleach @17:49:27.36 AS.seek 0.820ml @17:49:30.70 delay 3 seconds @17:49:33.71 ATV.dial PCR @17:49:34.06 AS.reconfigure ASconfig @17:49:34.10 AS.seek empty @17:50:22.22 PCR.program :bleachProfile @17:50:22.28 PCR program should complete in < 2:45 @17:50:22.29 PCR.run 10:00 @17:53:20.50 PCR.off @17:53:20.54 AFV.select :carrierSolution @17:53:20.65 delay 2 seconds @17:53:22.66 AS.seek 1.000ml @17:54:21.30 delay 1 second @17:54:22.32 AFV.select :holdingCoil @17:54:22.42 delay 1 second @17:54:23.43 AS.reconfigure ASfast @17:54:23.48 AS.seek 0.000ml @17:54:51.22 delay 3 seconds @17:54:54.23 AFV.select :carrierSolution @17:54:54.33 delay 2 seconds @17:54:56.34 AS.reconfigure ASconfig @17:54:56.39 AS.seek 1.000ml @17:55:55.01 delay 1 second @17:55:56.02 AFV.select :holdingCoil @17:55:56.12 delay 1 second @17:55:57.13 AS.reconfigure ASfast @17:55:57.18 AS.seek 0.000ml @17:56:24.93 delay 3 seconds @17:56:27.94 AFV.select :carrierSolution @17:56:28.04 delay 2 seconds @17:56:30.05 AS.reconfigure ASconfig @17:56:30.09 AS.seek 1.000ml @17:57:28.70 delay 1 second @17:57:29.71 AFV.select :holdingCoil @17:57:29.81 delay 1 second @17:57:30.82 AS.reconfigure ASfast @17:57:30.87 AS.seek 0.000ml @17:57:58.62 delay 3 seconds @17:58:01.63 AFV.select :carrierSolution @17:58:01.73 delay 2 seconds @17:58:03.74 AS.reconfigure ASconfig @17:58:03.79 AS.seek 1.000ml @17:59:02.40 delay 1 second @17:59:03.41 AFV.select :holdingCoil @17:59:03.51 delay 1 second @17:59:04.52 AS.reconfigure ASfast @17:59:04.56 AS.seek 0.000ml @17:59:32.32 delay 3 seconds @17:59:35.33 AFV.select :carrierSolution @17:59:35.43 delay 2 seconds @17:59:37.44 AS.reconfigure ASconfig @17:59:37.49 AS.seek 1.000ml @18:00:36.05 delay 1 second @18:00:37.06 AFV.select :holdingCoil @18:00:37.16 delay 1 second @18:00:38.17 AS.reconfigure ASnorm @18:00:38.21 AS.seek 0.000ml @18:01:29.67 Setting up PCR for IPCap -- writing /var/log/esp/IPCap14feb2618h1000ml.pcr @18:01:29.68 Building PCR Slug from ASV with MM1 mix, PP2 primer @18:01:29.69 Priming with carrier @18:01:29.71 ATV.dial ARV @18:01:30.33 ARV.dial :carrier @18:01:30.61 AS.seek 0.500ml @18:01:56.53 AFV.select :carrierSolution @18:01:56.64 delay 2 seconds @18:01:58.65 AS.reconfigure ASconfig @18:01:58.69 AS.seek 1.000ml @18:02:28.12 delay 1 second @18:02:29.13 AFV.select :holdingCoil @18:02:29.23 delay 1 second @18:02:30.24 ATV.dial :waste @18:02:31.63 AS.reconfigure ASnorm @18:02:31.67 AS.seek empty @18:03:23.15 AFV.select :carrierSolution @18:03:23.26 delay 2 seconds @18:03:25.27 AS.reconfigure ASconfig @18:03:25.32 AS.seek 0.500ml @18:03:54.81 delay 1 second @18:03:55.82 AFV.select :holdingCoil @18:03:55.92 Building Slug @18:03:55.93 ATV.dial :ARV @18:03:57.28 delay 2 seconds @18:03:59.29 ARV.dial :air1 @18:04:00.06 delay 2 seconds @18:04:02.12 AS.reconfigure ASslow2 @18:04:02.16 AS.seek 0.506ml @18:04:08.51 delay 2 seconds @18:04:10.53 ARV.dial :MM1 @18:04:11.13 delay 2 seconds @18:04:13.14 Adding MM1 @18:04:13.21 AS.seek 0.524ml @18:04:31.68 delay 25 seconds @18:04:56.69 ARV.dial :PP2 @18:04:57.03 Adding PP2 @18:04:57.04 delay 2 seconds @18:04:59.10 AS.seek 0.530ml @18:05:05.35 delay 10 seconds @18:05:15.37 ARV.dial :air2 @18:05:15.81 adding ARV air @18:05:15.82 delay 2 seconds @18:05:17.87 AS.seek 0.536ml @18:05:24.16 delay 5 seconds @18:05:29.17 ARV.dial :carrier @18:05:30.19 delay 7 seconds @18:05:37.75 AS.seek 0.594ml @18:06:37.08 delay 5 seconds @18:06:42.09 ATV.dial :ASV @18:06:42.43 delay 2 seconds @18:06:44.49 AS.seek 0.600ml @18:06:50.86 delay 4 seconds @18:06:54.87 ATV.dial ARV @18:06:55.21 delay 2 seconds @18:06:57.22 ARV.dial :carrier @18:06:57.23 delay 2 seconds @18:06:59.29 AS.seek 0.650ml @18:07:50.38 delay 4 seconds @18:07:54.39 ATV.dial PCR @18:07:54.91 ASV.dial :waste1 @18:07:54.92 ARV.dial :air1 @18:07:55.28 Positioning Slug by 0.324ml @18:07:55.35 AS.seek 0.326ml @18:13:25.95 delay 5 seconds @18:13:30.96 ATV.dial ARV @18:13:31.48 ASV.dial :PCRlock @18:13:32.20 delay 5 seconds @18:13:37.21 Running IPCap Profile59C thermocycling @18:13:37.22 PCR.program Profile59C @18:13:37.34 Profile59C should complete in < 1:39:08 @18:13:37.35 PCR.run 2:08:55 @19:40:00.28 PCR.off @19:40:00.81 PCR for IPCap completed -- closed /var/log/esp/IPCap14feb2618h1000ml.pcr @19:40:00.82 Full MFB Decontamination @19:40:00.84 Decon extraction column and decon AIV @19:40:00.86 SPE.hold 2:46:40,55.0C @19:40:00.90 ASV.dial :SPEdelivery @19:40:01.74 delay 20 seconds @19:40:21.75 AFV.select :carrierSolution @19:40:21.85 delay 2 seconds @19:40:23.86 AS.reconfigure ASconfig @19:40:23.91 AS.seek 0.500ml @19:40:34.35 delay 1 second @19:40:35.36 AFV.select :holdingCoil @19:40:35.46 ATV.dial :air1 @19:40:36.97 AS.reconfigure ASslow1 @19:40:37.01 AS.seek 0.520ml @19:40:41.87 ATV.dial :ASV @19:40:43.15 ASV.dial :SPErecovery @19:40:43.87 AS.seek 0.620ml @19:41:04.51 ATV.dial :waste @19:41:05.99 AS.reconfigure ASnorm @19:41:06.04 AS.seek empty @19:41:38.19 AFV.select :holdingCoil @19:41:38.20 ATV.dial :waste @19:41:38.22 AS.reconfigure ASconfig @19:41:38.27 AS.seek 0.000ml @19:41:38.54 AFV.select :carrierSolution @19:41:38.64 delay 2 seconds @19:41:40.65 AS.seek 0.500ml @19:42:10.07 delay 1 second @19:42:11.08 AFV.select :holdingCoil @19:42:11.18 ATV.dial :air3 @19:42:11.76 AS.reconfigure ASslow1 @19:42:11.80 AS.seek 0.520ml @19:42:16.13 ATV.dial :air3 @19:42:16.21 AS.seek 0.530ml @19:42:18.51 delay 2 seconds @19:42:20.52 ATV.dial :bleach @19:42:20.99 AS.seek 0.710ml @19:42:57.97 delay 3 seconds @19:43:00.99 ATV.dial :air3 @19:43:01.32 delay 3 seconds @19:43:04.38 AS.seek 0.735ml @19:43:09.74 delay 3 seconds @19:43:12.76 ATV.dial :bleach @19:43:13.26 AS.seek 0.720ml @19:43:16.57 delay 3 seconds @19:43:19.59 ATV.dial ASV @19:43:20.49 ASV.dial :SPErecovery @19:43:20.57 AS.seek 0.365ml @19:44:33.22 ATV.dial :waste @19:44:34.68 AS.reconfigure ASnorm @19:44:34.73 AS.seek empty @19:44:53.77 ATV.dial :air3 @19:44:54.42 AS.reconfigure ASslow1 @19:44:54.46 AS.seek 0.009ml @19:44:56.81 delay 2 seconds @19:44:58.82 ATV.dial :bleach @19:44:59.22 AS.seek 0.579ml @19:46:55.75 delay 3 seconds @19:46:58.77 ATV.dial :air3 @19:46:59.11 delay 3 seconds @19:47:02.17 AS.seek 0.604ml @19:47:07.54 delay 3 seconds @19:47:10.55 ATV.dial :bleach @19:47:10.94 AS.seek 0.589ml @19:47:14.26 delay 3 seconds @19:47:17.27 ATV.dial :ASV @19:47:18.15 ASV.dial :wasteR @19:47:18.93 AS.seek 0.000ml @19:49:19.47 delay 1:00 @19:50:19.48 ATV.dial ASV @19:50:19.49 AFV.select :carrierSolution @19:50:19.59 delay 2 seconds @19:50:21.60 AS.reconfigure ASconfig @19:50:21.65 AS.seek 1.000ml @19:51:20.19 delay 1 second @19:51:21.20 AFV.select :holdingCoil @19:51:21.30 delay 1 second @19:51:22.31 ASV.dial :wasteR @19:51:22.32 AS.reconfigure ASslow1 @19:51:22.37 AS.seek 0.000ml @19:54:46.66 delay 10 seconds @19:54:56.67 ATV.dial :air3 @19:54:57.79 AS.seek 0.010ml @19:55:00.14 delay 2 seconds @19:55:02.15 ATV.dial :bleach @19:55:02.65 AS.seek 0.580ml @19:56:59.14 delay 3 seconds @19:57:02.16 ATV.dial :air3 @19:57:02.50 delay 3 seconds @19:57:05.56 AS.seek 0.605ml @19:57:10.92 delay 3 seconds @19:57:13.93 ATV.dial :bleach @19:57:14.42 AS.seek 0.590ml @19:57:17.75 delay 3 seconds @19:57:20.76 ATV.dial ASV @19:57:21.75 ASV.dial :SPEdelivery @19:57:22.24 AS.seek 0.000ml @19:59:22.81 delay 1:00 @20:00:22.82 AFV.select :carrierSolution @20:00:22.92 delay 2 seconds @20:00:24.93 AS.reconfigure ASconfig @20:00:24.98 AS.seek 1.000ml @20:01:23.57 delay 1 second @20:01:24.59 AFV.select :holdingCoil @20:01:24.70 delay 1 second @20:01:25.71 ATV.dial ASV @20:01:25.72 ASV.dial :SPEdelivery @20:01:25.74 AS.reconfigure ASslow1 @20:01:25.79 AS.seek 0.000ml @20:04:50.03 delay 10 seconds @20:05:00.04 ATV.dial :air2 @20:05:00.77 AS.reconfigure ASslow0 @20:05:00.81 AS.seek 0.800ml @20:06:22.76 delay 3 seconds @20:06:25.77 ATV.dial :ASV @20:06:26.50 ASV.dial :SPEdelivery @20:06:26.52 AS.reconfigure ASslow1 @20:06:26.57 AS.seek 0.030ml @20:09:03.88 delay 20 seconds @20:09:23.89 ATV.dial :waste,via => air3 @20:09:25.33 AS.reconfigure ASconfig @20:09:25.37 AS.seek empty @20:09:27.47 SPE.coast @20:09:27.56 Removing Bleach from Mixing Coil @20:09:27.57 AFV.select :carrierSolution @20:09:27.67 delay 2 seconds @20:09:29.68 AS.seek 0.500ml @20:09:59.17 delay 1 second @20:10:00.18 AFV.select :holdingCoil @20:10:00.28 ATV.dial :ASV @20:10:01.72 ASV.dial :SPErecovery @20:10:02.42 AS.reconfigure ASslow1 @20:10:02.47 AS.seek 0.730ml @20:10:49.64 ASV.dial :wasteD @20:10:50.16 AS.reconfigure ASnorm @20:10:50.20 AS.seek 0.000ml @20:11:27.82 AFV.select :carrierSolution @20:11:27.92 delay 2 seconds @20:11:29.93 AS.reconfigure ASconfig @20:11:29.98 AS.seek 0.500ml @20:11:59.43 delay 1 second @20:12:00.44 AFV.select :holdingCoil @20:12:00.55 ATV.dial ASV @20:12:00.56 ASV.dial :SPErecovery @20:12:01.54 AS.reconfigure ASslow0 @20:12:01.58 AS.seek 0.300ml @20:12:22.27 ATV.dial :air2 @20:12:23.04 AS.reconfigure ASslow1 @20:12:23.08 AS.seek 0.320ml @20:12:27.43 ATV.dial ASV @20:12:28.20 AS.reconfigure ASslow0 @20:12:28.24 AS.seek 0.570ml @20:12:53.99 ASV.dial :wasteD @20:12:54.50 AS.reconfigure ASnorm @20:12:54.55 AS.seek 0.000ml @20:13:24.02 AFV.select :carrierSolution @20:13:24.13 delay 2 seconds @20:13:26.14 AS.reconfigure ASconfig @20:13:26.19 AS.seek 0.500ml @20:13:55.62 delay 1 second @20:13:56.63 AFV.select :holdingCoil @20:13:56.74 ATV.dial ASV @20:13:56.75 ASV.dial :SPErecovery @20:13:57.74 AS.reconfigure ASslow0 @20:13:57.79 AS.seek 0.300ml @20:14:18.51 ATV.dial :air2 @20:14:19.27 AS.reconfigure ASslow1 @20:14:19.31 AS.seek 0.320ml @20:14:23.68 ATV.dial ASV @20:14:24.54 AS.reconfigure ASslow0 @20:14:24.59 AS.seek 0.570ml @20:14:50.38 ASV.dial :wasteD @20:14:50.89 AS.reconfigure ASnorm @20:14:50.93 AS.seek 0.000ml @20:15:20.38 ATV.dial :air2 @20:15:21.09 AS.reconfigure ASslow0 @20:15:21.13 AS.seek 0.300ml @20:15:52.06 ATV.dial :ASV @20:15:52.77 ASV.dial :wasteD @20:15:52.79 AS.reconfigure ASconfig @20:15:52.84 AS.seek 0.050ml @20:16:07.72 ATV.dial :waste @20:16:09.15 AS.seek 0.000ml @20:16:12.34 ASV.dial :SPEdelivery @20:16:13.35 deconLysateINT @20:16:13.36 ATV.dial :air3 @20:16:14.02 AS.reconfigure ASslow1 @20:16:14.06 AS.seek 0.010ml @20:16:16.42 delay 2 seconds @20:16:18.43 ATV.dial :bleach @20:16:18.82 AS.seek 0.960ml @20:19:32.81 delay 3 seconds @20:19:35.82 ATV.dial :air3 @20:19:36.17 delay 3 seconds @20:19:39.23 AS.seek 0.985ml @20:19:44.59 delay 3 seconds @20:19:47.61 ATV.dial :bleach @20:19:48.11 AS.seek 0.970ml @20:19:51.45 delay 3 seconds @20:19:54.46 ATV.dial :AIV @20:19:55.65 AIV.dial :coilToMFB @20:19:55.66 AWV.select :waste @20:19:55.67 AFV.select :carrierSolution @20:19:55.78 delay 2 seconds @20:19:57.80 AS.reconfigure ASconfig @20:19:57.90 AS.seek 1.000ml @20:20:00.06 delay 1 second @20:20:01.07 AFV.select :holdingCoil @20:20:01.17 delay 1 second @20:20:02.18 AS.reconfigure ASslow0 @20:20:02.23 AS.seek empty @20:21:44.63 AFV.select :carrierSolution @20:21:44.74 delay 2 seconds @20:21:46.75 AS.reconfigure ASconfig @20:21:46.80 AS.seek 1.000ml @20:22:45.47 delay 1 second @20:22:46.49 AFV.select :holdingCoil @20:22:46.59 delay 1 second @20:22:47.60 AS.reconfigure ASslow0 @20:22:47.65 AS.seek empty @20:24:30.09 AFV.select :carrierSolution @20:24:30.19 delay 2 seconds @20:24:32.20 AS.reconfigure ASconfig @20:24:32.25 AS.seek 1.000ml @20:25:30.90 delay 1 second @20:25:31.92 AFV.select :holdingCoil @20:25:32.02 delay 1 second @20:25:33.03 AS.reconfigure ASslow0 @20:25:33.07 AS.seek empty @20:27:15.50 ATV.dial :air2 @20:27:16.18 AS.reconfigure ASslow1 @20:27:16.22 AS.seek 0.009ml @20:27:18.58 AS.reconfigure ASslow0 @20:27:18.63 AS.seek 1.000ml @20:27:26.68 Session Ended @20:28:59.98 ATV.dial AIV @20:29:00.49 AS.seek 0.020ml @20:30:40.78 ATV.dial :waste @20:30:42.04 AS.seek 0.000ml @20:30:44.37 AWV.select :air @20:30:44.51 Cleaning PCR @20:30:44.52 AFV.select :carrierSolution @20:30:44.61 delay 2 seconds @20:30:46.62 AS.reconfigure ASconfig @20:30:46.65 AS.seek 0.500ml @20:31:16.09 delay 1 second @20:31:17.10 AFV.select :holdingCoil @20:31:17.19 ASV.dial :waste1 @20:31:17.78 ATV.dial :air3 @20:31:18.43 AS.reconfigure ASslow1 @20:31:18.47 AS.seek 0.510ml @20:31:20.76 delay 2 seconds @20:31:22.77 ATV.dial :bleach @20:31:23.14 AS.seek 0.810ml @20:32:25.09 delay 3 seconds @20:32:28.10 ATV.dial :air3 @20:32:28.52 delay 3 seconds @20:32:31.58 AS.seek 0.835ml @20:32:36.93 delay 3 seconds @20:32:39.94 ATV.dial :bleach @20:32:40.43 AS.seek 0.820ml @20:32:43.74 delay 3 seconds @20:32:46.75 ARV.dial :waste @20:32:47.24 delay 3 seconds @20:32:50.25 ATV.dial :ARV @20:32:50.99 AS.reconfigure ASconfig @20:32:51.03 AS.seek 0.470ml @20:33:11.73 AFV.select :carrierSolution @20:33:11.82 delay 2 seconds @20:33:13.83 AS.seek 1.000ml @20:33:45.04 delay 1 second @20:33:46.05 AFV.select :holdingCoil @20:33:46.14 delay 1 second @20:33:47.15 AS.reconfigure ASfast @20:33:47.18 AS.seek 0.000ml @20:34:14.91 AFV.select :carrierSolution @20:34:15.01 delay 2 seconds @20:34:17.02 AS.reconfigure ASconfig @20:34:17.05 AS.seek 1.000ml @20:35:15.60 delay 1 second @20:35:16.61 AFV.select :holdingCoil @20:35:16.70 delay 1 second @20:35:17.71 AS.reconfigure ASfast @20:35:17.74 AS.seek 0.000ml @20:35:45.47 AFV.select :carrierSolution @20:35:45.57 delay 2 seconds @20:35:47.58 AS.reconfigure ASconfig @20:35:47.61 AS.seek 1.000ml @20:36:46.20 delay 1 second @20:36:47.21 AFV.select :holdingCoil @20:36:47.30 delay 1 second @20:36:48.31 AS.reconfigure ASfast @20:36:48.34 AS.seek 0.000ml @20:37:16.08 ARV.dial between carrier and air1 @20:37:16.50 ATV.dial :air3 @20:37:17.41 AS.reconfigure ASslow1 @20:37:17.45 AS.seek 0.010ml @20:37:19.78 delay 2 seconds @20:37:21.79 ATV.dial :bleach @20:37:22.17 AS.seek 0.810ml @20:40:05.59 delay 3 seconds @20:40:08.60 ATV.dial :air3 @20:40:09.02 delay 3 seconds @20:40:12.08 AS.seek 0.835ml @20:40:17.46 delay 3 seconds @20:40:20.47 ATV.dial :bleach @20:40:20.95 AS.seek 0.820ml @20:40:24.21 delay 3 seconds @20:40:27.22 ATV.dial PCR @20:40:27.55 AS.reconfigure ASconfig @20:40:27.58 AS.seek empty @20:41:15.68 PCR.program :bleachProfile @20:41:15.74 PCR program should complete in < 2:45 @20:41:15.75 PCR.run 10:00 @20:44:16.97 PCR.off @20:44:17.00 AFV.select :carrierSolution @20:44:17.09 delay 2 seconds @20:44:19.10 AS.seek 1.000ml @20:45:17.76 delay 1 second @20:45:18.77 AFV.select :holdingCoil @20:45:18.86 delay 1 second @20:45:19.87 AS.reconfigure ASfast @20:45:19.91 AS.seek 0.000ml @20:45:47.65 delay 3 seconds @20:45:50.66 AFV.select :carrierSolution @20:45:50.75 delay 2 seconds @20:45:52.76 AS.reconfigure ASconfig @20:45:52.79 AS.seek 1.000ml @20:46:51.39 delay 1 second @20:46:52.40 AFV.select :holdingCoil @20:46:52.49 delay 1 second @20:46:53.50 AS.reconfigure ASfast @20:46:53.53 AS.seek 0.000ml @20:47:21.25 delay 3 seconds @20:47:24.26 AFV.select :carrierSolution @20:47:24.35 delay 2 seconds @20:47:26.36 AS.reconfigure ASconfig @20:47:26.39 AS.seek 1.000ml @20:48:24.98 delay 1 second @20:48:25.99 AFV.select :holdingCoil @20:48:26.08 delay 1 second @20:48:27.09 AS.reconfigure ASfast @20:48:27.12 AS.seek 0.000ml @20:48:54.83 delay 3 seconds @20:48:57.84 AFV.select :carrierSolution @20:48:57.93 delay 2 seconds @20:48:59.94 AS.reconfigure ASconfig @20:48:59.97 AS.seek 1.000ml @20:49:58.56 delay 1 second @20:49:59.57 AFV.select :holdingCoil @20:49:59.67 delay 1 second @20:50:00.68 AS.reconfigure ASfast @20:50:00.71 AS.seek 0.000ml @20:50:28.45 delay 3 seconds @20:50:31.46 AFV.select :carrierSolution @20:50:31.55 delay 2 seconds @20:50:33.56 AS.reconfigure ASconfig @20:50:33.59 AS.seek 1.000ml @20:51:32.18 delay 1 second @20:51:33.19 AFV.select :holdingCoil @20:51:33.28 delay 1 second @20:51:34.29 AS.reconfigure ASnorm @20:51:34.32 AS.seek 0.000ml @20:52:25.78 AFV.select :carrierSolution @20:52:25.87 delay 2 seconds @20:52:27.88 AS.reconfigure ASconfig @20:52:27.91 AS.seek 1.000ml @20:53:26.52 delay 1 second @20:53:27.53 AFV.select :holdingCoil @20:53:27.62 delay 1 second @20:53:28.63 ATV.dial :waste @20:53:29.61 AS.reconfigure ASnorm @20:53:29.64 AS.seek 0.500ml @20:53:55.57 ATV.dial :waste2 @20:53:56.64 AS.seek 0.000ml @20:54:22.54
Cleaning PCR @20:54:22.55 AFV.select :carrierSolution @20:54:22.64 delay 2 seconds @20:54:24.65 AS.reconfigure ASconfig @20:54:24.68 AS.seek 0.500ml @20:54:54.11 delay 1 second @20:54:55.12 AFV.select :holdingCoil @20:54:55.21 ASV.dial :waste1 @20:54:55.22 ATV.dial :air3 @20:54:57.16 AS.reconfigure ASslow1 @20:54:57.19 AS.seek 0.510ml @20:54:59.47 delay 2 seconds @20:55:01.48 ATV.dial :bleach @20:55:01.85 AS.seek 0.810ml @20:56:03.28 delay 3 seconds @20:56:06.30 ATV.dial :air3 @20:56:06.63 delay 3 seconds @20:56:09.69 AS.seek 0.835ml @20:56:15.03 delay 3 seconds @20:56:18.04 ATV.dial :bleach @20:56:18.41 AS.seek 0.820ml @20:56:21.73 delay 3 seconds @20:56:24.75 ARV.dial :waste @20:56:25.16 delay 3 seconds @20:56:28.17 ATV.dial :ARV @20:56:28.99 AS.reconfigure ASconfig @20:56:29.03 AS.seek 0.470ml @20:56:49.70 AFV.select :carrierSolution @20:56:49.79 delay 2 seconds @20:56:51.80 AS.seek 1.000ml @20:57:23.03 delay 1 second @20:57:24.04 AFV.select :holdingCoil @20:57:24.13 delay 1 second @20:57:25.14 AS.reconfigure ASfast @20:57:25.18 AS.seek 0.000ml @20:57:52.90 AFV.select :carrierSolution @20:57:52.99 delay 2 seconds @20:57:55.00 AS.reconfigure ASconfig @20:57:55.03 AS.seek 1.000ml @20:58:53.63 delay 1 second @20:58:54.64 AFV.select :holdingCoil @20:58:54.73 delay 1 second @20:58:55.74 AS.reconfigure ASfast @20:58:55.77 AS.seek 0.000ml @20:59:23.52 AFV.select :carrierSolution @20:59:23.61 delay 2 seconds @20:59:25.62 AS.reconfigure ASconfig @20:59:25.65 AS.seek 1.000ml @21:00:24.21 delay 1 second @21:00:25.22 AFV.select :holdingCoil @21:00:25.31 delay 1 second @21:00:26.32 AS.reconfigure ASfast @21:00:26.35 AS.seek 0.000ml @21:00:54.07 ARV.dial between carrier and air1 @21:00:54.47 ATV.dial :air3 @21:00:55.39 AS.reconfigure ASslow1 @21:00:55.43 AS.seek 0.010ml @21:00:57.76 delay 2 seconds @21:00:59.77 ATV.dial :bleach @21:01:00.26 AS.seek 0.810ml @21:03:43.68 delay 3 seconds @21:03:46.69 ATV.dial :air3 @21:03:47.01 delay 3 seconds @21:03:50.07 AS.seek 0.835ml @21:03:55.43 delay 3 seconds @21:03:58.44 ATV.dial :bleach @21:03:58.92 AS.seek 0.820ml @21:04:02.21 delay 3 seconds @21:04:05.22 ATV.dial PCR @21:04:05.55 AS.reconfigure ASconfig @21:04:05.58 AS.seek empty @21:04:53.71 PCR.program :bleachProfile @21:04:53.77 PCR program should complete in < 2:45 @21:04:53.78 PCR.run 10:00 @21:07:54.99 PCR.off @21:07:55.02 AFV.select :carrierSolution @21:07:55.11 delay 2 seconds @21:07:57.12 AS.seek 1.000ml @21:08:55.75 delay 1 second @21:08:56.76 AFV.select :holdingCoil @21:08:56.85 delay 1 second @21:08:57.86 AS.reconfigure ASfast @21:08:57.89 AS.seek 0.000ml @21:09:25.61 delay 3 seconds @21:09:28.63 AFV.select :carrierSolution @21:09:28.72 delay 2 seconds @21:09:30.73 AS.reconfigure ASconfig @21:09:30.76 AS.seek 1.000ml @21:10:29.39 delay 1 second @21:10:30.40 AFV.select :holdingCoil @21:10:30.49 delay 1 second @21:10:31.50 AS.reconfigure ASfast @21:10:31.53 AS.seek 0.000ml @21:10:59.24 delay 3 seconds @21:11:02.25 AFV.select :carrierSolution @21:11:02.34 delay 2 seconds @21:11:04.35 AS.reconfigure ASconfig @21:11:04.39 AS.seek 1.000ml @21:12:02.96 delay 1 second @21:12:03.97 AFV.select :holdingCoil @21:12:04.06 delay 1 second @21:12:05.07 AS.reconfigure ASfast @21:12:05.10 AS.seek 0.000ml @21:12:32.79 delay 3 seconds @21:12:35.81 AFV.select :carrierSolution @21:12:35.90 delay 2 seconds @21:12:37.91 AS.reconfigure ASconfig @21:12:37.94 AS.seek 1.000ml @21:13:36.57 delay 1 second @21:13:37.58 AFV.select :holdingCoil @21:13:37.67 delay 1 second @21:13:38.68 AS.reconfigure ASfast @21:13:38.71 AS.seek 0.000ml @21:14:06.44 delay 3 seconds @21:14:09.46 AFV.select :carrierSolution @21:14:09.55 delay 2 seconds @21:14:11.56 AS.reconfigure ASconfig @21:14:11.59 AS.seek 1.000ml @21:15:10.19 delay 1 second @21:15:11.20 AFV.select :holdingCoil @21:15:11.29 delay 1 second @21:15:12.30 AS.reconfigure ASnorm @21:15:12.33 AS.seek 0.000ml @21:16:03.75 Completed {wqPCR} begun 13:35:45 @21:16:03.76 Asleep after completing 14feb25sccwrp assays @21:16:03.77 GoodNews.email "neo completed 14feb25sccwrp mission",Subject => "Ready to Recover" @21:16:05.98 GoodNews.email "",Subject => "Asleep after completing 14feb25sccwrp assays" hostname: ESPneo: Host name lookup failure @18:28:04.45PST02-Mar-14 ESP2 v3.20 I2C Gateway v3.01 @18:28:07.48
Configuration for neo by maro@mbari.org /home/esp/esp2/shallow/neo/configure.rb @18:28:07.57 Configuration for MFB10 by preston@mbari.org /home/esp/esp2/mfb/MFB10/configure.rb @18:28:10.71 Sleepy.power :raw,:ON @18:28:11.40 SPE.coast @18:28:11.44 SPE.configure SPEconfig @18:28:11.47 AS.stop @18:28:11.50 AS.configure ASconfig @18:28:11.53 CC.stop @18:28:11.55 CC.configure CCconfig @18:28:11.58 CS.stop @18:28:11.60 CS.configure CSconfig @18:28:11.63 PC.stop @18:28:11.65 PC.configure PCconfig @18:28:11.68 PS.stop @18:28:11.70 PS.configure PSconfig @18:28:11.76 Forearm.stop @18:28:11.78 Forearm.configure LooseForearm @18:28:11.81 Elbow.stop @18:28:11.83 Elbow.configure LooseElbow @18:28:11.89 SC.stop @18:28:11.91 SC.configure SCloose @18:28:11.94 SE.stop @18:28:11.96 SE.configure SEconfig @18:28:11.99 SS.stop @18:28:12.02 SS.configure SSconfig @18:28:12.05 CH.coast @18:28:12.09 CH.configure CHconfig @18:28:12.12 PH.coast @18:28:12.16 PH.configure PHconfig @18:28:12.20 PCR.off @18:28:12.22 PCR.pidtemp= 50,0.1,0,300,25,25 @18:28:12.25 PCR.caltemp= 6.6661,0.0344,0.0 @18:28:12.28 All dwarves are running firmware version 3.74 @18:28:12.89 GoodNews.email "Starting w/pucks in tube 2\n ",Subject => "14mar02sccwrp begun" @18:28:13.10
Asleep til +0:10 @18:28:13.12 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @18:28:13.16 Sleeping until 18:28 @18:28:13.17 Retry #1 of 10 after 5:00 @18:28:13.19 Sleepy.power :raw,:OFF @18:28:13.20 Sleepy.power :dwarf,:OFF Shutdown @18:28:22.64
Powering up Dwarves @18:28:27.82 Sleepy.power :raw,:ON @18:28:28.34 SPE.configure SPEconfig @18:28:28.36 AS.configure ASconfig @18:28:28.39 CC.configure CCconfig @18:28:28.42 CS.configure CSconfig @18:28:28.45 PC.configure PCconfig @18:28:28.48 PS.configure PSconfig @18:28:28.51 Forearm.configure LooseForearm @18:28:28.54 Elbow.configure LooseElbow @18:28:28.57 SC.configure SCloose @18:28:28.60 SE.configure SEconfig @18:28:28.63 SS.configure SSconfig @18:28:28.66 CH.configure CHconfig @18:28:28.68 PH.configure PHconfig @18:28:28.71 Valve.reset @18:28:29.32 PCR.off @18:28:29.35 PCR.pidtemp= 50,0.1,0,300,25,25 @18:28:29.38 PCR.caltemp= 6.6661,0.0344,0.0 @18:28:29.48 Intake.select :close @18:28:29.51 Exhaust.select :close @18:28:29.52 CTV.dial :air @18:28:29.56 PSV.dial :air @18:28:29.59
PC.reconfigure PCconfig @18:28:29.64 PC.home.seek open @18:28:29.69 PS.reconfigure PSconfig @18:28:29.72 CSV.dial :waste1 @18:28:29.76 PS.home.seek 0.000ml @18:28:29.90 SSV.dial :spit,avoiding => exhaust @18:28:30.01 CS.reconfigure CSfast @18:28:30.04 SS.reconfigure SSconfig @18:28:30.14 CS.home.seek 0.000ml @18:28:30.17 SS.home.seek 0.00ml @18:28:30.23 CC.reconfigure CCconfig @18:28:30.27 CC.home.seek open @18:28:40.00 PS.coast @18:28:40.08
CC.seek open @18:28:40.10 PC.seek open @18:28:40.27
Forearm.reconfigure LooseForearm @18:28:40.31 Forearm.home.seek retract @18:28:41.05 Hand.grip @18:28:41.07
Elbow.jog -1500 @18:28:41.55 Elbow.reconfigure LooseElbow @18:28:41.59 Elbow.home.seek clear @18:28:45.04 SE.reconfigure SEconfig @18:28:45.07 SE.home.seek down @18:28:45.64 SC.reconfigure SCsloppy @18:28:45.68 SC.home.seek initial @18:28:49.39 Forearm.seek retract @18:28:49.45 Elbow.seek hammer @18:28:50.62 Forearm.reconfigure TightForearm @18:28:50.67 Forearm.seek 2 @18:28:51.81 Forearm.seek retract @18:28:52.82 Forearm.reconfigure LooseForearm @18:28:53.23PST02-Mar-14 ===> STARTED wqPCR (phase #1) <=== @18:28:53.31 Starting mfb @18:28:53.32 pcrBAC sampling at most 1000ml, wcr at most 1000ml @18:28:53.33 Loading sh1 puck from tube 2 into Collection Clamp @18:28:53.42 Hand.release @18:28:53.47 Readying the MFB @18:28:53.48 Homing MFB Syringe @18:28:53.49 AFV.select :holdingCoil @18:28:53.62 ATV.dial :air2 @18:28:54.31
SC.reconfigure SCloose @18:28:54.45 Elbow.reconfigure TightElbow @18:28:54.48 Elbow.seek 2 @18:28:54.53 AS.reconfigure ASconfig @18:28:54.59 AS.home.seek home @18:28:54.74
Forearm.reconfigure TightForearm @18:28:54.77 Forearm.seek 2 + 800 counts @18:28:55.86 SE.reconfigure SEpick @18:28:55.91 SE.seek beamBreak @18:28:56.79 SE.configure SEconfig @18:28:56.91 Elbow.seek 2 @18:28:57.07 Forearm.seek 2 + 300 counts @18:28:57.42 Forearm.reconfigure LooseForearm @18:28:57.46 Hand.grip @18:28:58.17 SE.seek down @18:28:58.30
Forearm.seek retract @18:28:58.97 SE.coast @18:28:59.26
Elbow.seek CC @18:29:00.74 Elbow.reconfigure LooseElbow @18:29:00.82 Forearm.reconfigure TightForearm @18:29:00.85 Forearm.seek CC @18:29:01.95 CC.seek closed @18:29:02.63 AFV.select :carrierSolution @18:29:02.73 delay 2 seconds @18:29:04.77 AS.seek 1.000ml @18:29:05.69
Collection Clamp onSpring/offSpring for 5/22 samples, on/off torque 1.94 @18:29:05.70 Hand.release @18:29:06.44 Forearm.reconfigure LooseForearm @18:29:06.47 Forearm.seek retract @18:29:07.39 SC.coast @18:29:07.42 collectSample 1000ml w/bubble point of 55psi @18:29:07.43 Priming 7ml Intake.select :close @18:29:07.44 Exhaust.select :close @18:29:07.45 SS.hold @18:29:07.62 Calibrating pressure and temperature sensors @18:29:07.64 CTV.dial :air SSV.dial :spit,avoiding => exhaust @18:29:07.66 SS.reconfigure SSfast @18:29:07.69 SS.seek 5.00ml @18:29:10.72 SSV.dial CBV @18:29:10.74
CBV.dial SSV @18:29:12.07 delay 3.8 seconds @18:29:15.95 SSV.dial :spit @18:29:17.24 SS.seek 0.00ml @18:29:19.94 SSV.dial CBV @18:29:21.22 CTV.dial :intake @18:29:21.93 SSV.dial CBV @18:29:21.94
CBV.dial SSV @18:29:21.95 Allowing 55s for 7.0ml of remaining 7.0ml @18:29:21.96 Intake.select :open @18:29:24.09 Intake @34.8psia, vacuum from 20.0 to 24.0psi @18:29:24.10 SS.reconfigure forSampling @18:29:24.14 SS.seek 7.00ml @18:29:29.92 Stroke completed -- waiting for pressure to equalize ... @18:29:30.56 Intake.select :close @18:29:30.69 SSV.dial :exhaust @18:29:31.42 Exhaust.select :open @18:29:31.51 SS.reconfigure SSexhale @18:29:31.55 SS.seek 0.00ml @18:29:34.98 Exhaust.select :close @18:29:35.07 Sampling 1000.0ml @18:29:35.08 Intake.select :close @18:29:35.09 Exhaust.select :close @18:29:35.10 SS.reconfigure SSconfig @18:29:35.13 SS.hold @18:29:35.28 CTV.dial :intake @18:29:35.29 SSV.dial CBV @18:29:35.31
CBV.dial SSV @18:29:36.07 Allowing 185s for 25.0ml of remaining 1000.0ml Intake.select :open @18:29:38.20 Intake @34.5psia, vacuum from 20.0 to 24.0psi @18:29:38.21 SS.reconfigure forSampling @18:29:38.25 SS.seek 25.00ml @18:29:41.55 Vacuum too high -- waiting up to 40s @18:29:41.81 Intake @28.7psia, vacuum from 20.0 to 24.0psi @18:29:41.82 SS.reconfigure forSampling @18:29:41.85 SS.seek 25.00ml @18:29:50.24 Vacuum too high -- waiting up to 40s @18:29:50.93 Intake @29.5psia, vacuum from 20.0 to 24.0psi @18:29:50.94 SS.reconfigure forSampling @18:29:50.97 SS.seek 25.00ml @18:29:56.03 Stroke completed -- waiting for pressure to equalize ... @18:29:56.53 delay 1 second @18:29:56.68
Intake.select :close @18:29:56.81 SSV.dial :exhaust @18:29:57.55 Exhaust.select :open @18:29:57.58 AFV.select :holdingCoil @18:29:57.64
SS.reconfigure SSexhale @18:29:57.68 SS.seek 0.00ml @18:29:57.71 delay 1 second @18:29:58.72 ATV.dial :waste @18:29:59.62 AS.reconfigure ASnorm @18:29:59.65 AS.home.seek empty @18:30:06.33
Exhaust.select :close @18:30:06.42 SSV.dial CBV @18:30:06.44
CBV.dial SSV @18:30:07.20 Allowing 185s for 25.0ml of remaining 975.0ml @18:30:07.21 Intake.select :open @18:30:09.34 Intake @34.1psia, vacuum from 20.0 to 24.0psi @18:30:09.35 SS.reconfigure forSampling @18:30:09.39 SS.seek 25.00ml @18:30:12.36 Vacuum too high -- waiting up to 40s @18:30:12.62 Intake @29.3psia, vacuum from 20.0 to 24.0psi @18:30:12.63 SS.reconfigure forSampling @18:30:12.66 SS.seek 25.00ml @18:30:28.82 Stroke completed -- waiting for pressure to equalize ... @18:30:29.46 Intake.select :close @18:30:29.59 SSV.dial :exhaust @18:30:30.34 Exhaust.select :open @18:30:30.44 SS.reconfigure SSexhale @18:30:30.48 SS.seek 0.00ml @18:30:39.20 Exhaust.select :close @18:30:39.29 SSV.dial CBV @18:30:39.31
CBV.dial SSV @18:30:40.07 Allowing 185s for 25.0ml of remaining 950.0ml @18:30:40.08 Intake.select :open @18:30:42.21 Intake @34.0psia, vacuum from 20.0 to 24.0psi @18:30:42.87 SS.reconfigure forSampling @18:30:42.91 SS.seek 25.00ml @18:30:46.30 Vacuum too high -- waiting up to 40s @18:30:46.57 Intake @29.5psia, vacuum from 20.0 to 24.0psi @18:30:46.58 SS.reconfigure forSampling @18:30:46.61 SS.seek 25.00ml @18:30:52.95 ATV.dial :waste @18:30:52.96 ARV.dial between carrier and air1 @18:30:53.03 ASV.dial :waste1 @18:31:04.26
Stroke completed -- waiting for pressure to equalize ... @18:31:04.90 Intake.select :close @18:31:05.03 SSV.dial :exhaust @18:31:05.76 Exhaust.select :open @18:31:05.86 SS.reconfigure SSexhale @18:31:05.90 SS.seek 0.00ml @18:31:14.48 Exhaust.select :close @18:31:14.57 SSV.dial CBV @18:31:14.59
CBV.dial SSV @18:31:15.34 Allowing 185s for 25.0ml of remaining 925.0ml @18:31:15.35 Intake.select :open @18:31:17.48 Intake @34.0psia, vacuum from 20.0 to 24.0psi @18:31:17.49 SS.reconfigure forSampling @18:31:17.53 SS.seek 25.00ml @18:31:39.01 Stroke completed -- waiting for pressure to equalize ... @18:31:39.65 Intake.select :close @18:31:39.78 SSV.dial :exhaust @18:31:40.52 Exhaust.select :open @18:31:40.61 SS.reconfigure SSexhale @18:31:40.65 SS.seek 0.00ml @18:31:49.13 Exhaust.select :close @18:31:49.22 SSV.dial CBV @18:31:49.24
CBV.dial SSV @18:31:49.99 Allowing 185s for 25.0ml of remaining 900.0ml @18:31:50.00 Intake.select :open @18:31:52.13 Intake @34.0psia, vacuum from 20.0 to 24.0psi @18:31:52.14 SS.reconfigure forSampling @18:31:52.18 SS.seek 25.00ml @18:32:13.71 Stroke completed -- waiting for pressure to equalize ... @18:32:14.35 Intake.select :close @18:32:14.48 SSV.dial :exhaust @18:32:15.21 Exhaust.select :open @18:32:15.30 SS.reconfigure SSexhale @18:32:15.34 SS.seek 0.00ml @18:32:23.83 Exhaust.select :close @18:32:23.92 SSV.dial CBV @18:32:23.94
CBV.dial SSV @18:32:24.69 Allowing 185s for 25.0ml of remaining 875.0ml @18:32:24.70 Intake.select :open @18:32:26.83 Intake @34.1psia, vacuum from 20.0 to 24.0psi @18:32:26.84 SS.reconfigure forSampling @18:32:26.88 SS.seek 25.00ml @18:32:47.86 Stroke completed -- waiting for pressure to equalize ... @18:32:48.50 Intake.select :close @18:32:48.63 SSV.dial :exhaust @18:32:49.36 Exhaust.select :open @18:32:49.45 SS.reconfigure SSexhale @18:32:49.49 SS.seek 0.00ml @18:32:58.07 Exhaust.select :close @18:32:58.16 SSV.dial CBV @18:32:58.18
CBV.dial SSV @18:32:58.94 Allowing 185s for 25.0ml of remaining 850.0ml Intake.select :open @18:33:01.07 Intake @34.0psia, vacuum from 20.0 to 24.0psi @18:33:01.08 SS.reconfigure forSampling @18:33:01.12 SS.seek 25.00ml @18:33:13.18 GoodNews.email "Starting w/pucks in tube 2\n ",Subject => "14mar02sccwrp begun" @18:33:13.20 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @18:33:13.21 Retry #2 of 10 after 5:00 @18:33:22.74
Stroke completed -- waiting for pressure to equalize ... @18:33:23.38 Intake.select :close @18:33:23.51 SSV.dial :exhaust @18:33:24.25 Exhaust.select :open @18:33:24.34 SS.reconfigure SSexhale @18:33:24.38 SS.seek 0.00ml @18:33:32.91 Exhaust.select :close @18:33:33.00 SSV.dial CBV @18:33:33.02
CBV.dial SSV @18:33:33.78 Allowing 185s for 25.0ml of remaining 825.0ml Intake.select :open @18:33:35.91 Intake @34.2psia, vacuum from 20.0 to 24.0psi @18:33:35.92 SS.reconfigure forSampling @18:33:35.96 SS.seek 25.00ml @18:33:57.59 Stroke completed -- waiting for pressure to equalize ... @18:33:58.23 Intake.select :close @18:33:58.36 SSV.dial :exhaust @18:33:59.09 Exhaust.select :open @18:33:59.19 SS.reconfigure SSexhale @18:33:59.23 SS.seek 0.00ml @18:34:08.01 Exhaust.select :close @18:34:08.10 SSV.dial CBV @18:34:08.12
CBV.dial SSV @18:34:08.87 Allowing 185s for 25.0ml of remaining 800.0ml @18:34:08.88 Intake.select :open @18:34:11.01 Intake @34.0psia, vacuum from 20.0 to 24.0psi @18:34:11.02 SS.reconfigure forSampling @18:34:11.06 SS.seek 25.00ml @18:34:32.45 Stroke completed -- waiting for pressure to equalize ... @18:34:33.09 Intake.select :close @18:34:33.22 SSV.dial :exhaust @18:34:34.06 Exhaust.select :open @18:34:34.16 SS.reconfigure SSexhale @18:34:34.20 SS.seek 0.00ml @18:34:42.74 Exhaust.select :close @18:34:42.83 SSV.dial CBV @18:34:42.85
CBV.dial SSV @18:34:43.60 Allowing 185s for 25.0ml of remaining 775.0ml @18:34:43.61 Intake.select :open @18:34:45.74 Intake @34.1psia, vacuum from 20.0 to 24.0psi @18:34:45.75 SS.reconfigure forSampling @18:34:45.79 SS.seek 25.00ml @18:35:07.05 Stroke completed -- waiting for pressure to equalize ... @18:35:07.69 Intake.select :close @18:35:07.82 SSV.dial :exhaust @18:35:08.55 Exhaust.select :open @18:35:08.65 SS.reconfigure SSexhale @18:35:08.69 SS.seek 0.00ml @18:35:17.19 Exhaust.select :close @18:35:17.28 SSV.dial CBV @18:35:17.30
CBV.dial SSV @18:35:18.05 Allowing 185s for 25.0ml of remaining 750.0ml @18:35:18.06 Intake.select :open @18:35:20.21 Intake @34.1psia, vacuum from 20.0 to 24.0psi @18:35:20.22 SS.reconfigure forSampling @18:35:20.26 SS.seek 25.00ml @18:35:41.69 Stroke completed -- waiting for pressure to equalize ... @18:35:42.33 Intake.select :close @18:35:42.46 SSV.dial :exhaust @18:35:43.19 Exhaust.select :open @18:35:43.29 SS.reconfigure SSexhale @18:35:43.33 SS.seek 0.00ml @18:35:51.94 Exhaust.select :close @18:35:52.03 SSV.dial CBV @18:35:52.05
CBV.dial SSV @18:35:52.80 Allowing 185s for 25.0ml of remaining 725.0ml @18:35:52.81 Intake.select :open @18:35:54.94 Intake @34.1psia, vacuum from 20.0 to 24.0psi @18:35:54.95 SS.reconfigure forSampling @18:35:54.99 SS.seek 25.00ml @18:36:15.92 Stroke completed -- waiting for pressure to equalize ... @18:36:16.56 Intake.select :close @18:36:16.69 SSV.dial :exhaust @18:36:17.43 Exhaust.select :open @18:36:17.52 SS.reconfigure SSexhale @18:36:17.56 SS.seek 0.00ml @18:36:26.24 Exhaust.select :close @18:36:26.33 SSV.dial CBV @18:36:26.35
CBV.dial SSV @18:36:27.10 Allowing 185s for 25.0ml of remaining 700.0ml @18:36:27.11 Intake.select :open @18:36:29.24 Intake @34.0psia, vacuum from 20.0 to 24.0psi @18:36:29.25 SS.reconfigure forSampling @18:36:29.29 SS.seek 25.00ml @18:36:50.77 Stroke completed -- waiting for pressure to equalize ... @18:36:51.41 Intake.select :close @18:36:51.54 SSV.dial :exhaust @18:36:52.28 Exhaust.select :open @18:36:52.38 SS.reconfigure SSexhale @18:36:52.42 SS.seek 0.00ml @18:37:00.91 Exhaust.select :close @18:37:01.00 SSV.dial CBV @18:37:01.02
CBV.dial SSV @18:37:01.77 Allowing 185s for 25.0ml of remaining 675.0ml @18:37:01.78 Intake.select :open @18:37:03.91 Intake @34.0psia, vacuum from 20.0 to 24.0psi @18:37:03.92 SS.reconfigure forSampling @18:37:03.96 SS.seek 25.00ml @18:37:26.06 Stroke completed -- waiting for pressure to equalize ... @18:37:26.70 Intake.select :close @18:37:26.83 SSV.dial :exhaust @18:37:27.56 Exhaust.select :open @18:37:27.67 SS.reconfigure SSexhale @18:37:27.71 SS.seek 0.00ml @18:37:36.23 Exhaust.select :close @18:37:36.32 SSV.dial CBV @18:37:36.34
CBV.dial SSV @18:37:37.09 Allowing 185s for 25.0ml of remaining 650.0ml @18:37:37.10 Intake.select :open @18:37:39.23 Intake @34.0psia, vacuum from 20.0 to 24.0psi @18:37:39.24 SS.reconfigure forSampling @18:37:39.28 SS.seek 25.00ml @18:38:00.78 Stroke completed -- waiting for pressure to equalize ... @18:38:01.42 Intake.select :close @18:38:01.56 SSV.dial :exhaust @18:38:02.29 Exhaust.select :open @18:38:02.39 SS.reconfigure SSexhale @18:38:02.43 SS.seek 0.00ml @18:38:10.82 Exhaust.select :close @18:38:10.91 SSV.dial CBV @18:38:10.93
CBV.dial SSV @18:38:11.68 Allowing 185s for 25.0ml of remaining 625.0ml @18:38:11.69 Intake.select :open @18:38:13.51 GoodNews.email "Starting w/pucks in tube 2\n ",Subject => "14mar02sccwrp begun" @18:38:13.53 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @18:38:13.54 Retry #3 of 10 after 5:00 @18:38:13.82
Intake @34.0psia, vacuum from 20.0 to 24.0psi @18:38:13.83 SS.reconfigure forSampling @18:38:13.87 SS.seek 25.00ml @18:38:17.56 Vacuum too high -- waiting up to 40s @18:38:17.82 Intake @30.5psia, vacuum from 20.0 to 24.0psi @18:38:17.83 SS.reconfigure forSampling @18:38:17.87 SS.seek 25.00ml @18:38:38.82 Stroke completed -- waiting for pressure to equalize ... @18:38:39.46 Intake.select :close @18:38:39.59 SSV.dial :exhaust @18:38:40.32 Exhaust.select :open @18:38:40.42 SS.reconfigure SSexhale @18:38:40.46 SS.seek 0.00ml @18:38:48.95 Exhaust.select :close @18:38:49.04 SSV.dial CBV @18:38:49.06
CBV.dial SSV @18:38:49.81 Allowing 185s for 25.0ml of remaining 600.0ml @18:38:49.82 Intake.select :open @18:38:51.95 Intake @34.0psia, vacuum from 20.0 to 24.0psi @18:38:51.96 SS.reconfigure forSampling @18:38:52.00 SS.seek 25.00ml @18:39:17.77 Stroke completed -- waiting for pressure to equalize ... @18:39:18.41 Intake.select :close @18:39:18.54 SSV.dial :exhaust @18:39:19.27 Exhaust.select :open @18:39:19.36 SS.reconfigure SSexhale @18:39:19.40 SS.seek 0.00ml @18:39:28.16 Exhaust.select :close @18:39:28.25 SSV.dial CBV @18:39:28.27
CBV.dial SSV @18:39:29.02 Allowing 185s for 25.0ml of remaining 575.0ml @18:39:29.03 Intake.select :open @18:39:31.16 Intake @34.0psia, vacuum from 20.0 to 24.0psi @18:39:31.17 SS.reconfigure forSampling @18:39:31.21 SS.seek 25.00ml @18:39:55.90 Stroke completed -- waiting for pressure to equalize ... @18:39:56.54 Intake.select :close @18:39:56.67 SSV.dial :exhaust @18:39:57.41 Exhaust.select :open @18:39:57.50 SS.reconfigure SSexhale @18:39:57.54 SS.seek 0.00ml @18:40:06.13 Exhaust.select :close @18:40:06.22 SSV.dial CBV @18:40:06.24
CBV.dial SSV @18:40:06.99 Allowing 185s for 25.0ml of remaining 550.0ml @18:40:07.00 Intake.select :open @18:40:09.13 Intake @33.9psia, vacuum from 20.0 to 24.0psi @18:40:09.14 SS.reconfigure forSampling @18:40:09.18 SS.seek 25.00ml @18:40:34.27 Stroke completed -- waiting for pressure to equalize ... @18:40:34.91 Intake.select :close @18:40:35.04 SSV.dial :exhaust @18:40:35.77 Exhaust.select :open @18:40:35.87 SS.reconfigure SSexhale @18:40:35.91 SS.seek 0.00ml @18:40:44.44 Exhaust.select :close @18:40:44.53 SSV.dial CBV @18:40:44.55
CBV.dial SSV @18:40:45.30 Allowing 185s for 25.0ml of remaining 525.0ml @18:40:45.31 Intake.select :open @18:40:47.44 Intake @34.0psia, vacuum from 20.0 to 24.0psi @18:40:47.45 SS.reconfigure forSampling @18:40:47.49 SS.seek 25.00ml @18:41:12.53 Stroke completed -- waiting for pressure to equalize ... @18:41:13.17 Intake.select :close @18:41:13.30 SSV.dial :exhaust @18:41:14.03 Exhaust.select :open @18:41:14.12 SS.reconfigure SSexhale @18:41:14.16 SS.seek 0.00ml @18:41:22.69 Exhaust.select :close @18:41:22.78 SSV.dial CBV @18:41:22.80
CBV.dial SSV @18:41:23.55 Allowing 185s for 25.0ml of remaining 500.0ml @18:41:23.56 Intake.select :open @18:41:25.69 Intake @33.9psia, vacuum from 20.0 to 24.0psi @18:41:25.70 SS.reconfigure forSampling @18:41:25.74 SS.seek 25.00ml @18:41:51.08 Stroke completed -- waiting for pressure to equalize ... @18:41:51.72 Intake.select :close @18:41:51.85 SSV.dial :exhaust @18:41:52.59 Exhaust.select :open @18:41:52.68 SS.reconfigure SSexhale @18:41:52.72 SS.seek 0.00ml @18:42:01.22 Exhaust.select :close @18:42:01.31 SSV.dial CBV @18:42:01.33
CBV.dial SSV @18:42:02.08 Allowing 185s for 25.0ml of remaining 475.0ml @18:42:02.09 Intake.select :open @18:42:04.22 Intake @34.0psia, vacuum from 20.0 to 24.0psi @18:42:04.23 SS.reconfigure forSampling @18:42:04.27 SS.seek 25.00ml @18:42:29.01 Stroke completed -- waiting for pressure to equalize ... @18:42:29.65 Intake.select :close @18:42:29.78 SSV.dial :exhaust @18:42:30.52 Exhaust.select :open @18:42:30.61 SS.reconfigure SSexhale @18:42:30.65 SS.seek 0.00ml @18:42:39.40 Exhaust.select :close @18:42:39.49 SSV.dial CBV @18:42:39.51
CBV.dial SSV @18:42:40.26 Allowing 185s for 25.0ml of remaining 450.0ml @18:42:40.27 Intake.select :open @18:42:42.40 Intake @34.0psia, vacuum from 20.0 to 24.0psi @18:42:42.41 SS.reconfigure forSampling @18:42:42.45 SS.seek 25.00ml @18:43:07.53 Stroke completed -- waiting for pressure to equalize ... @18:43:08.17 Intake.select :close @18:43:08.30 SSV.dial :exhaust @18:43:09.04 Exhaust.select :open @18:43:09.14 SS.reconfigure SSexhale @18:43:09.18 SS.seek 0.00ml @18:43:13.54 GoodNews.email "Starting w/pucks in tube 2\n ",Subject => "14mar02sccwrp begun" @18:43:13.56 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @18:43:13.57 Retry #4 of 10 after 5:00 @18:43:17.70
Exhaust.select :close @18:43:17.79 SSV.dial CBV @18:43:17.81
CBV.dial SSV @18:43:18.56 Allowing 185s for 25.0ml of remaining 425.0ml @18:43:18.57 Intake.select :open @18:43:20.70 Intake @33.9psia, vacuum from 20.0 to 24.0psi @18:43:20.71 SS.reconfigure forSampling @18:43:20.75 SS.seek 25.00ml @18:43:45.49 Stroke completed -- waiting for pressure to equalize ... @18:43:46.70 Intake.select :close @18:43:46.84 SSV.dial :exhaust @18:43:47.57 Exhaust.select :open @18:43:47.67 SS.reconfigure SSexhale @18:43:47.71 SS.seek 0.00ml @18:43:56.24 Exhaust.select :close @18:43:56.33 SSV.dial CBV @18:43:56.35
CBV.dial SSV @18:43:57.10 Allowing 185s for 25.0ml of remaining 400.0ml @18:43:57.11 Intake.select :open @18:43:59.24 Intake @33.9psia, vacuum from 20.0 to 24.0psi @18:43:59.25 SS.reconfigure forSampling @18:43:59.29 SS.seek 25.00ml @18:44:23.99 Stroke completed -- waiting for pressure to equalize ... @18:44:24.63 Intake.select :close @18:44:24.77 SSV.dial :exhaust @18:44:25.51 Exhaust.select :open @18:44:25.62 SS.reconfigure SSexhale @18:44:25.66 SS.seek 0.00ml @18:44:34.01 Exhaust.select :close @18:44:34.10 SSV.dial CBV @18:44:34.12
CBV.dial SSV @18:44:34.87 Allowing 185s for 25.0ml of remaining 375.0ml @18:44:34.88 Intake.select :open @18:44:37.01 Intake @33.9psia, vacuum from 20.0 to 24.0psi @18:44:37.02 SS.reconfigure forSampling @18:44:37.06 SS.seek 25.00ml @18:45:01.82 Stroke completed -- waiting for pressure to equalize ... @18:45:02.46 Intake.select :close @18:45:02.59 SSV.dial :exhaust @18:45:03.33 Exhaust.select :open @18:45:03.43 SS.reconfigure SSexhale @18:45:03.47 SS.seek 0.00ml @18:45:11.96 Exhaust.select :close @18:45:12.05 SSV.dial CBV @18:45:12.07
CBV.dial SSV @18:45:12.82 Allowing 185s for 25.0ml of remaining 350.0ml @18:45:12.83 Intake.select :open @18:45:14.96 Intake @34.0psia, vacuum from 20.0 to 24.0psi @18:45:14.97 SS.reconfigure forSampling @18:45:15.01 SS.seek 25.00ml @18:45:39.74 Stroke completed -- waiting for pressure to equalize ... @18:45:40.38 Intake.select :close @18:45:40.52 SSV.dial :exhaust @18:45:41.25 Exhaust.select :open @18:45:41.35 SS.reconfigure SSexhale @18:45:41.39 SS.seek 0.00ml @18:45:49.86 Exhaust.select :close @18:45:49.95 SSV.dial CBV @18:45:49.97
CBV.dial SSV @18:45:50.72 Allowing 185s for 25.0ml of remaining 325.0ml @18:45:50.73 Intake.select :open @18:45:52.86 Intake @33.9psia, vacuum from 20.0 to 24.0psi @18:45:52.87 SS.reconfigure forSampling @18:45:52.91 SS.seek 25.00ml @18:46:18.25 Stroke completed -- waiting for pressure to equalize ... @18:46:18.89 Intake.select :close @18:46:19.02 SSV.dial :exhaust @18:46:19.75 Exhaust.select :open @18:46:19.85 SS.reconfigure SSexhale @18:46:19.89 SS.seek 0.00ml @18:46:28.75 Exhaust.select :close @18:46:28.84 SSV.dial CBV @18:46:28.86
CBV.dial SSV @18:46:29.61 Allowing 185s for 25.0ml of remaining 300.0ml @18:46:29.62 Intake.select :open @18:46:31.75 Intake @33.7psia, vacuum from 20.0 to 24.0psi @18:46:31.76 SS.reconfigure forSampling @18:46:31.80 SS.seek 25.00ml @18:46:56.47 Stroke completed -- waiting for pressure to equalize ... @18:46:57.11 Intake.select :close @18:46:57.24 SSV.dial :exhaust @18:46:57.98 Exhaust.select :open @18:46:58.08 SS.reconfigure SSexhale @18:46:58.12 SS.seek 0.00ml @18:47:06.94 Exhaust.select :close @18:47:07.03 SSV.dial CBV @18:47:07.05
CBV.dial SSV @18:47:07.80 Allowing 185s for 25.0ml of remaining 275.0ml @18:47:07.81 Intake.select :open @18:47:09.94 Intake @33.7psia, vacuum from 20.0 to 24.0psi @18:47:09.95 SS.reconfigure forSampling @18:47:09.99 SS.seek 25.00ml @18:47:34.59 Stroke completed -- waiting for pressure to equalize ... @18:47:35.23 Intake.select :close @18:47:35.36 SSV.dial :exhaust @18:47:36.09 Exhaust.select :open @18:47:36.19 SS.reconfigure SSexhale @18:47:36.23 SS.seek 0.00ml @18:47:44.56 Exhaust.select :close @18:47:44.65 SSV.dial CBV @18:47:44.67
CBV.dial SSV @18:47:45.42 Allowing 185s for 25.0ml of remaining 250.0ml @18:47:45.43 Intake.select :open @18:47:47.56 Intake @33.7psia, vacuum from 20.0 to 24.0psi @18:47:47.57 SS.reconfigure forSampling @18:47:47.61 SS.seek 25.00ml @18:48:12.81 Stroke completed -- waiting for pressure to equalize ... @18:48:13.44 Intake.select :close @18:48:13.58 SSV.dial :exhaust @18:48:13.60 GoodNews.email "Starting w/pucks in tube 2\n ",Subject => "14mar02sccwrp begun" @18:48:13.62 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @18:48:13.65 Retry #5 of 10 after 5:00 @18:48:14.37
Exhaust.select :open @18:48:14.47 SS.reconfigure SSexhale @18:48:14.51 SS.seek 0.00ml @18:48:22.99 Exhaust.select :close @18:48:23.08 SSV.dial CBV @18:48:23.10
CBV.dial SSV @18:48:23.85 Allowing 185s for 25.0ml of remaining 225.0ml @18:48:23.86 Intake.select :open @18:48:25.99 Intake @33.9psia, vacuum from 20.0 to 24.0psi @18:48:26.00 SS.reconfigure forSampling @18:48:26.04 SS.seek 25.00ml @18:48:50.79 Stroke completed -- waiting for pressure to equalize ... @18:48:51.43 Intake.select :close @18:48:51.56 SSV.dial :exhaust @18:48:52.74 Exhaust.select :open @18:48:52.84 SS.reconfigure SSexhale @18:48:52.88 SS.seek 0.00ml @18:49:01.68 Exhaust.select :close @18:49:01.77 SSV.dial CBV @18:49:01.79
CBV.dial SSV @18:49:02.54 Allowing 185s for 25.0ml of remaining 200.0ml @18:49:02.55 Intake.select :open @18:49:04.68 Intake @33.8psia, vacuum from 20.0 to 24.0psi @18:49:04.69 SS.reconfigure forSampling @18:49:04.73 SS.seek 25.00ml @18:49:29.40 Stroke completed -- waiting for pressure to equalize ... @18:49:30.04 Intake.select :close @18:49:30.17 SSV.dial :exhaust @18:49:30.91 Exhaust.select :open @18:49:31.01 SS.reconfigure SSexhale @18:49:31.05 SS.seek 0.00ml @18:49:39.54 Exhaust.select :close @18:49:39.63 SSV.dial CBV @18:49:39.65
CBV.dial SSV @18:49:40.40 Allowing 185s for 25.0ml of remaining 175.0ml @18:49:40.41 Intake.select :open @18:49:42.56 Intake @33.7psia, vacuum from 20.0 to 24.0psi @18:49:42.57 SS.reconfigure forSampling @18:49:42.61 SS.seek 25.00ml @18:50:08.10 Stroke completed -- waiting for pressure to equalize ... @18:50:08.74 Intake.select :close @18:50:08.88 SSV.dial :exhaust @18:50:09.61 Exhaust.select :open @18:50:09.71 SS.reconfigure SSexhale @18:50:09.75 SS.seek 0.00ml @18:50:18.25 Exhaust.select :close @18:50:18.34 SSV.dial CBV @18:50:18.36
CBV.dial SSV @18:50:19.12 Allowing 185s for 25.0ml of remaining 150.0ml Intake.select :open @18:50:21.25 Intake @33.8psia, vacuum from 20.0 to 24.0psi @18:50:21.26 SS.reconfigure forSampling @18:50:21.30 SS.seek 25.00ml @18:50:46.49 Stroke completed -- waiting for pressure to equalize ... @18:50:47.13 Intake.select :close @18:50:47.26 SSV.dial :exhaust @18:50:48.00 Exhaust.select :open @18:50:48.10 SS.reconfigure SSexhale @18:50:48.14 SS.seek 0.00ml @18:50:56.66 Exhaust.select :close @18:50:56.75 SSV.dial CBV @18:50:56.77
CBV.dial SSV @18:50:57.52 Allowing 185s for 25.0ml of remaining 125.0ml @18:50:57.54 Intake.select :open @18:50:59.68 Intake @33.7psia, vacuum from 20.0 to 24.0psi @18:50:59.69 SS.reconfigure forSampling @18:50:59.73 SS.seek 25.00ml @18:51:24.85 Stroke completed -- waiting for pressure to equalize ... @18:51:25.49 Intake.select :close @18:51:25.62 SSV.dial :exhaust @18:51:26.35 Exhaust.select :open @18:51:26.45 SS.reconfigure SSexhale @18:51:26.49 SS.seek 0.00ml @18:51:34.99 Exhaust.select :close @18:51:35.08 SSV.dial CBV @18:51:35.10
CBV.dial SSV @18:51:35.85 Allowing 185s for 25.0ml of remaining 100.0ml @18:51:35.86 Intake.select :open @18:51:37.99 Intake @33.6psia, vacuum from 20.0 to 24.0psi @18:51:38.00 SS.reconfigure forSampling @18:51:38.04 SS.seek 25.00ml @18:52:03.51 Stroke completed -- waiting for pressure to equalize ... @18:52:04.15 Intake.select :close @18:52:04.28 SSV.dial :exhaust @18:52:05.02 Exhaust.select :open @18:52:05.12 SS.reconfigure SSexhale @18:52:05.16 SS.seek 0.00ml @18:52:13.65 Exhaust.select :close @18:52:13.74 SSV.dial CBV @18:52:13.76
CBV.dial SSV @18:52:14.51 Allowing 185s for 25.0ml of remaining 75.0ml @18:52:14.52 Intake.select :open @18:52:16.65 Intake @33.6psia, vacuum from 20.0 to 24.0psi @18:52:16.66 SS.reconfigure forSampling @18:52:16.70 SS.seek 25.00ml @18:52:42.15 Stroke completed -- waiting for pressure to equalize ... @18:52:42.79 Intake.select :close @18:52:42.93 SSV.dial :exhaust @18:52:43.66 Exhaust.select :open @18:52:43.76 SS.reconfigure SSexhale @18:52:43.80 SS.seek 0.00ml @18:52:52.32 Exhaust.select :close @18:52:52.41 SSV.dial CBV @18:52:52.43
CBV.dial SSV @18:52:53.18 Allowing 185s for 25.0ml of remaining 50.0ml @18:52:53.19 Intake.select :open @18:52:55.32 Intake @33.6psia, vacuum from 20.0 to 24.0psi @18:52:55.33 SS.reconfigure forSampling @18:52:55.37 SS.seek 25.00ml @18:53:13.68 GoodNews.email "Starting w/pucks in tube 2\n ",Subject => "14mar02sccwrp begun" @18:53:13.70 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @18:53:13.71 Retry #6 of 10 after 5:00 @18:53:20.11
Stroke completed -- waiting for pressure to equalize ... @18:53:20.74 Intake.select :close @18:53:20.88 SSV.dial :exhaust @18:53:21.61 Exhaust.select :open @18:53:21.71 SS.reconfigure SSexhale @18:53:21.75 SS.seek 0.00ml @18:53:30.18 Exhaust.select :close @18:53:30.27 SSV.dial CBV @18:53:30.29
CBV.dial SSV @18:53:31.05 Allowing 185s for 25.0ml of remaining 25.0ml @18:53:31.06 Intake.select :open @18:53:33.21 Intake @33.6psia, vacuum from 20.0 to 24.0psi @18:53:33.22 SS.reconfigure forSampling @18:53:33.26 SS.seek 24.98ml @18:53:57.95 Stroke completed -- waiting for pressure to equalize ... @18:53:58.58 Intake.select :close @18:53:58.72 SSV.dial :exhaust @18:53:59.46 Exhaust.select :open @18:53:59.56 SS.reconfigure SSexhale @18:53:59.60 SS.seek 0.00ml @18:54:08.61 Exhaust.select :close @18:54:08.70 Sampled 1000.0ml @18:54:08.71 collectSample: Complete @18:54:08.72 Evacuating Puck (bubblePt=55.0psi, delay=15.0s) @18:54:08.73 Intake.select :close @18:54:08.78 CSV.dial :waste @18:54:08.80 CS.seek 0.000ml @18:54:08.91 CSV.dial :air @18:54:08.93 SSV.dial CBV,avoiding => exhaust @18:54:08.96
CBV.dial SSV @18:54:09.68 CS.seek 10.000ml @18:54:10.81
CTV.dial :air @18:54:11.56 delay 15 seconds @18:54:26.56 SSV.dial :spit,avoiding => exhaust @18:54:27.87 CTV.dial CSV,avoiding => intake @18:54:27.89
SS.seek 0.00ml @18:54:27.98 SSV.dial CBV,avoiding => exhaust @18:54:33.19 CBV.dial between CSV and waste2b @18:54:34.13 CTV.dial CSV,avoiding => air @18:54:34.15
CSV.dial CTV @18:54:34.58 CS.reconfigure CSpressurize @18:54:34.61 CS.seek 0.000ml @18:54:59.31 BubblePoint: Intake @70.0psia, Outlet @15.2psia @18:54:59.32 SS.reconfigure SSconfig @18:54:59.35 SS.seek 10.00ml @18:55:05.31 delay 15 seconds @18:55:20.41 Evacuated: Intake @68.5psia, Outlet @-0.5psia @18:55:20.42 SSV.dial :spit,avoiding => exhaust @18:55:21.71 SS.reconfigure SSexhale @18:55:21.74 SS.seek 0.00ml @18:55:26.70 SS.reconfigure SSfast @18:55:26.73 SS.seek 5.00ml @18:55:29.85 SSV.dial CBV,avoiding => exhaust @18:55:29.87
CBV.dial SSV @18:55:31.16 CTV.dial :air,avoiding => intake @18:55:32.46 CSV.dial CTV @18:55:32.47
SS.reconfigure SSconfig @18:55:32.51 SS.seek 10.00ml @18:55:33.47 CSV.dial :air @18:55:33.97 CS.reconfigure CSfast @18:55:34.00 CS.seek 0.000ml @18:55:35.61
SSV.dial :spit,avoiding => exhaust @18:55:35.85 CS.coast @18:55:36.91
SS.reconfigure SSexhale @18:55:36.94 SS.seek 0.00ml @18:55:42.43 SS.reconfigure SSfast @18:55:42.47 SS.seek 5.00ml @18:55:45.35 SSV.dial CBV,avoiding => exhaust @18:55:45.37
CBV.dial SSV @18:55:47.65 SSV.dial :spit,avoiding => exhaust @18:55:48.94 SS.reconfigure SSconfig @18:55:48.97 SS.seek 0.00ml @18:55:52.23 SS.coast @18:55:52.26 Evacuating Puck (bubblePt=55.0psi, delay=15.0s) Intake.select :close @18:55:52.32 CSV.dial :waste @18:55:52.41
SSV.dial :exhaust @18:55:53.07 CS.seek 0.000ml @18:55:53.15
Exhaust.select :open @18:55:53.26 SS.reconfigure SSexhale @18:55:53.30 SS.seek 0.00ml @18:55:53.34 CSV.dial :air @18:55:53.87
Exhaust.select :close @18:55:53.96 SSV.dial CBV,avoiding => exhaust @18:55:53.98
CBV.dial SSV @18:55:54.16 CS.seek 10.000ml @18:55:55.82
CTV.dial :air @18:55:55.83 delay 15 seconds @18:56:10.83 SSV.dial :spit,avoiding => exhaust @18:56:12.15 CTV.dial CSV,avoiding => intake @18:56:12.17
SS.seek 0.00ml @18:56:12.26 SSV.dial CBV,avoiding => exhaust @18:56:17.71 CBV.dial between CSV and waste2b @18:56:18.64 CSV.dial between air and CTV @18:56:19.97 CS.seek 8.116ml @18:56:25.08 CTV.dial CSV,avoiding => air @18:56:25.09
CSV.dial CTV @18:56:25.47 CS.reconfigure CSpressurize @18:56:25.50 CS.seek 0.000ml @18:56:46.60 CSV.dial :air @18:56:48.53 CS.reconfigure CSfast @18:56:48.56 CS.seek 10.000ml @18:57:11.86 CSV.dial between air and CTV @18:57:12.17 CS.seek 2.923ml @18:57:28.89 CTV.dial CSV,avoiding => air @18:57:28.90
CSV.dial CTV @18:57:29.19 CS.reconfigure CSpressurize @18:57:29.22 CS.seek 0.000ml @18:57:35.46 BubblePoint: Intake @70.9psia, Outlet @16.0psia @18:57:35.47 SS.reconfigure SSconfig @18:57:35.50 SS.seek 10.00ml @18:57:41.41 delay 15 seconds @18:57:56.51 Evacuated: Intake @69.8psia, Outlet @-0.5psia SSV.dial :spit,avoiding => exhaust @18:57:58.25 SS.reconfigure SSexhale @18:57:58.28 SS.seek 0.00ml @18:58:03.21 SS.reconfigure SSfast @18:58:03.25 SS.seek 5.00ml @18:58:06.17 SSV.dial CBV,avoiding => exhaust @18:58:06.19
CBV.dial SSV @18:58:07.51 CTV.dial :air,avoiding => intake @18:58:08.79 CSV.dial CTV @18:58:08.80
SS.reconfigure SSconfig @18:58:08.84 SS.seek 10.00ml @18:58:09.80 CSV.dial :air @18:58:10.32 CS.reconfigure CSfast @18:58:10.35 CS.seek 0.000ml @18:58:12.10
SSV.dial :spit,avoiding => exhaust @18:58:12.63 CS.coast @18:58:13.42
SS.reconfigure SSexhale @18:58:13.45 SS.seek 0.00ml @18:58:13.71 GoodNews.email "Starting w/pucks in tube 2\n ",Subject => "14mar02sccwrp begun" @18:58:13.73 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @18:58:13.74 Retry #7 of 10 after 5:00 @18:58:18.68
SS.reconfigure SSfast @18:58:18.72 SS.seek 5.00ml @18:58:21.84 SSV.dial CBV,avoiding => exhaust @18:58:21.86
CBV.dial SSV @18:58:24.14 SSV.dial :spit,avoiding => exhaust @18:58:25.43 SS.reconfigure SSconfig @18:58:25.46 SS.seek 0.00ml @18:58:28.55 SS.coast @18:58:28.58 Lysing: Adding 1.5ml lysis @18:58:28.59 CBV.dial between CSV and waste1,avoiding => DBV @18:58:30.06 CTV.dial CSV @18:58:31.33 CSV.dial CSR @18:58:32.11 CSR.select :lysis @18:58:32.21 CS.reconfigure CSconfig @18:58:32.25 CS.seek 1.000ml @18:58:35.66 delay 5 seconds @18:58:40.67 CSR.select :air @18:58:40.81 CS.seek 6.001ml @18:58:54.28 delay 2 seconds @18:58:56.29 CSV.dial :waste1 @18:58:57.56 CS.reconfigure CSfast @18:58:57.59 CS.seek empty @18:59:11.87 CSV.dial CSR @18:59:13.14 CSR.select :lysis @18:59:13.24 CS.reconfigure CSconfig @18:59:13.28 CS.seek 1.500ml @18:59:17.92 delay 5 seconds @18:59:22.92 CSR.select :air @18:59:23.06 CS.seek 4.500ml @18:59:31.56 delay 2 seconds @18:59:33.57 CSV.dial CTV @18:59:34.09 CS.seek 1.275ml @18:59:43.13 Heating sh1 puck from tube 2 in Collection Clamp to 85.0C for 5:00 @18:59:43.14 CH.seek 85.0C,8:00 @19:02:24.44 CH.hold 5:00,85.0C @19:03:13.74 GoodNews.email "Starting w/pucks in tube 2\n ",Subject => "14mar02sccwrp begun" @19:03:13.76 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @19:03:13.77 Retry #8 of 10 after 5:00 @19:03:57.04 Session Begun @19:03:57.07 -> Sleepy.cycleModemPower! 9 @19:03:57.09 Session Ended @19:07:24.54
Collection Puck Heater at 85.0C after lysing @19:07:24.55 CS.seek 0.000ml @19:07:28.61 CTV.dial between spare and air,avoiding => intake @19:07:29.61 CSV.dial :air @19:07:30.03 CS.seek 5.000ml @19:07:43.51 CSV.dial CTV @19:07:44.01 delay 2 seconds @19:07:46.02 CSV.dial :air @19:07:46.52 delay 2 seconds @19:07:48.53 CSV.dial CTV @19:07:49.04 delay 2 seconds @19:07:51.05 CSV.dial :air @19:07:51.55 delay 2 seconds @19:07:53.56 CS.seek 0.000ml @19:08:07.05 Recovering Lysate into Collection syringe @19:08:07.06 CSV.dial CBV @19:08:08.05 CBV.dial CSV @19:08:08.41 CS.seek 5.000ml @19:08:13.79 GoodNews.email "Starting w/pucks in tube 2\n ",Subject => "14mar02sccwrp begun" @19:08:13.81 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @19:08:13.82 Retry #9 of 10 after 5:00 @19:08:21.89
delay 5 seconds @19:08:26.90 CTV.dial :air @19:08:27.39 CS.seek 7.000ml @19:08:33.30 delay 5 seconds @19:08:38.32 CSV.dial :air2 @19:08:38.76 CS.seek 10.000ml @19:08:47.11 CSV.dial between air and CTV,avoiding => CBV @19:08:48.24 Lysing Complete @19:08:48.29 Storing sh1 puck in Collection Clamp to tube 1 @19:08:48.42 SC.reconfigure SCtight @19:08:48.45 SC.seek 1 @19:08:50.64 SC.reconfigure SCloose @19:08:50.81 Elbow.reconfigure TightElbow @19:08:50.85 Elbow.seek 1 @19:08:52.44 Forearm.reconfigure TightForearm @19:08:52.48 Forearm.seek 1 + 800 counts @19:08:53.54 SE.reconfigure SEpick @19:08:53.59 SE.seek beamBreak @19:08:56.38 SE.configure SEconfig @19:08:56.42 SE.seek place @19:08:56.87 Forearm.reconfigure LooseForearm @19:08:56.91 Forearm.seek retract @19:08:57.85 Elbow.seek CC @19:08:59.36 Elbow.reconfigure LooseElbow @19:08:59.45 Forearm.seek CC @19:09:00.39 CC.seek sealed @19:09:01.32 Hand.grip @19:09:02.07 CC.seek open @19:09:05.43 Forearm.seek retract @19:09:06.37 Elbow.reconfigure TightElbow @19:09:06.41 Elbow.seek 1 @19:09:08.53 Elbow.reconfigure LimpElbow @19:09:08.57 Forearm.seek 1 @19:09:09.52 Elbow.hold @19:09:09.57 Elbow.reconfigure TightElbow @19:09:09.61 Hand.release @19:09:10.41 Forearm.coast @19:09:10.44 Forearm.seek 1 + 339 counts @19:09:10.72 Forearm.reconfigure TightForearm @19:09:10.75 Forearm.seek 1 + 800 counts @19:09:10.97 SE.seek down @19:09:12.07
Forearm.reconfigure LooseForearm @19:09:12.11 Forearm.seek retract @19:09:13.67 SE.coast @19:09:13.70
SC.coast @19:09:13.73 Tranferring lysate to PRV @19:09:13.74 CSV.dial :air @19:09:14.05 CS.seek 10.000ml @19:09:14.11 CSV.dial between air and CTV @19:09:14.51 AIV.dial :bypass @19:09:14.58 Beginning samplePassC @19:09:14.59 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @19:09:14.73 Elbow.seek FlushPuck::Garage @19:09:16.47 Elbow.reconfigure LooseElbow @19:09:16.55 Forearm.seek garage @19:09:17.57 Hand.grip @19:09:18.41 Forearm.seek retract @19:09:19.43 Elbow.reconfigure TightElbow @19:09:19.53 Elbow.seek CC @19:09:22.36 Elbow.reconfigure LooseElbow @19:09:22.44 Forearm.reconfigure TightForearm @19:09:22.47 Forearm.seek CC @19:09:23.56 CC.seek closed @19:09:27.35 Collection Clamp onSpring/offSpring for 5/22 samples, on/off torque 1.99 @19:09:27.40 Hand.release @19:09:28.23 Forearm.reconfigure LooseForearm @19:09:28.26 Forearm.seek retract @19:09:29.20 AWV.select :air @19:09:29.21 AIV.dial :bypass @19:09:29.22 PSV.dial PSR @19:09:30.52 PSR.select :flush @19:09:30.62 PS.seek 1.500ml @19:09:46.64 delay 5 seconds @19:09:51.65 PSV.dial :waste1 @19:09:52.09 PS.seek 0.000ml @19:10:08.14 PSV.dial PSR @19:10:08.58 PSR.select :air @19:10:08.68 PS.seek 2.500ml @19:10:34.87 PRV.dial CBV @19:10:34.89
CBV.dial PRV @19:10:35.65 PSV.dial :mixloop @19:10:36.66 CTV.dial :air,avoiding => intake @19:10:36.67 PTV.dial :PRVmixing @19:10:36.75 PS.seek 0.000ml @19:11:02.98 PSV.dial :air @19:11:03.43 PS.seek 2.500ml @19:11:29.61 PSV.dial :mixloop @19:11:30.06 PS.seek 0.000ml @19:11:56.26 PSV.dial :air @19:11:56.71 PS.seek 2.500ml @19:12:22.92 PSV.dial :mixloop @19:12:23.37 PS.seek 0.000ml @19:12:49.64 Garaging FlushPuck in Collection Clamp @19:12:49.70 Elbow.reconfigure TightElbow @19:12:49.73 Elbow.seek CC @19:12:49.91 Elbow.reconfigure LooseElbow @19:12:49.99 Forearm.seek CC @19:12:50.96 CC.seek sealed @19:12:51.88 Hand.grip @19:12:52.56 CC.seek open @19:12:56.42 Forearm.seek retract @19:12:57.40 Elbow.reconfigure TightElbow @19:12:57.44 Elbow.seek garage @19:13:00.24 Elbow.reconfigure LooseElbow @19:13:00.32 Forearm.reconfigure TightForearm @19:13:00.35 Forearm.seek garage @19:13:01.53 Hand.release @19:13:02.35 Forearm.reconfigure LooseForearm @19:13:02.38 Forearm.seek retract @19:13:03.40 Connecting PS to CS to transfer sample @19:13:03.41 CBV.dial AIV @19:13:03.42 AIV.dial :bypass @19:13:03.43 PSV.dial :mixloop @19:13:03.44 PTV.dial :PRVmixing @19:13:03.45 PRV.dial AIV @19:13:03.46 CTV.dial :air,avoiding => intake @19:13:03.47 Loading lyfil puck from tube 2 into Collection Clamp @19:13:03.65 SC.reconfigure SCtight @19:13:03.68 SC.seek 2 @19:13:05.94 SC.reconfigure SCloose @19:13:06.12 Elbow.reconfigure TightElbow @19:13:06.15 Elbow.seek 2 @19:13:08.08 Forearm.reconfigure TightForearm @19:13:08.11 Forearm.seek 2 + 800 counts @19:13:09.17 SE.reconfigure SEpick @19:13:09.22 SE.seek beamBreak @19:13:10.34 SE.configure SEconfig @19:13:10.46 Elbow.seek 2 @19:13:10.70 Forearm.seek 2 + 300 counts @19:13:11.04 Forearm.reconfigure LooseForearm @19:13:11.08 Hand.grip @19:13:11.81 SE.seek down @19:13:11.95
Forearm.seek retract @19:13:12.66 SE.coast @19:13:12.91
Elbow.seek CC @19:13:13.83 GoodNews.email "Starting w/pucks in tube 2\n ",Subject => "14mar02sccwrp begun" @19:13:13.85 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @19:13:13.86 Retry #10 of 10 after 5:00 @19:13:14.45
Elbow.reconfigure LooseElbow @19:13:14.53 Forearm.reconfigure TightForearm @19:13:14.56 Forearm.seek CC @19:13:15.75 CC.seek closed @19:13:19.52 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 2.01 @19:13:19.53 Hand.release @19:13:20.34 Forearm.reconfigure LooseForearm @19:13:20.37 Forearm.seek retract @19:13:21.31 SC.coast @19:13:21.34 CTV.dial CSV,avoiding => intake @19:13:22.64 CSV.dial CTV @19:13:22.93 CS.seek 5.000ml @19:13:36.34 delay 1:20 @19:14:56.35 CS.seek 2.500ml @19:15:03.40 delay 40 seconds @19:15:43.41 CS.seek 0.000ml @19:15:50.56 delay 1:00 @19:16:50.57 PSV.dial :air @19:16:51.03 PS.seek 2.500ml @19:17:17.28 PSV.dial :mixloop @19:17:17.72 delay 3 seconds @19:17:20.73 PSV.dial :air @19:17:21.17 delay 2 seconds @19:17:23.18 PSV.dial :mixloop @19:17:23.61 delay 3 seconds @19:17:26.62 PSV.dial :waste1 @19:17:27.92 PS.seek empty @19:17:54.16 CBV.dial :waste1 @19:17:54.63 delay 5 seconds @19:17:59.64 CBV.dial AIV @19:18:00.10 delay 10 seconds @19:18:10.11 CBV.dial :waste1 @19:18:10.56 delay 10 seconds @19:18:13.86 GoodNews.email "Starting w/pucks in tube 2\n ",Subject => "14mar02sccwrp begun" @19:18:13.88 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @19:18:13.89 GoodNews.email "Then, in tube 2: wqPCR\n/home/esp/esp2/mission/14mar02sccwrp.rb:47",Subject => "Asleep until 18:28" @19:18:13.91 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @19:18:13.92 Retry #1 of 10 after 5:00 @19:18:20.57
CBV.dial AIV @19:18:21.02 delay 10 seconds @19:18:31.03 CTV.dial CSV,avoiding => intake @19:18:31.04 CBV.dial between AIV and waste1 @19:18:31.36 CSV.dial :air @19:18:31.87 CS.seek 10.000ml @19:18:58.05 CSV.dial CTV @19:18:58.56 CS.seek 0.000ml @19:19:24.64 CBV.dial :waste1 @19:19:24.94 delay 10 seconds @19:19:34.95 CTV.dial :air,avoiding => intake @19:19:36.28 Storing lyfil puck in Collection Clamp to tube 1 @19:19:36.41 SC.reconfigure SCtight @19:19:36.44 SC.seek 1 @19:19:38.61 SC.reconfigure SCloose @19:19:39.27 Elbow.reconfigure TightElbow @19:19:39.30 Elbow.seek 1 @19:19:40.91 Forearm.reconfigure TightForearm @19:19:40.94 Forearm.seek 1 + 800 counts @19:19:42.03 SE.reconfigure SEpick @19:19:42.08 SE.seek beamBreak @19:19:44.99 SE.configure SEconfig @19:19:45.11 Elbow.reconfigure LimpElbow @19:19:45.15 Forearm.reconfigure LooseForearm @19:19:45.19 Forearm.seek 1 @19:19:45.43 Elbow.hold @19:19:45.48 Elbow.reconfigure TightElbow @19:19:45.56 SE.seek 0.04 Pucks below Top Plate @19:19:45.85 Forearm.coast @19:19:45.88 Forearm.seek 1 + 343 counts @19:19:46.16 Forearm.reconfigure TightForearm @19:19:46.19 Forearm.seek 1 + 800 counts @19:19:46.36 SE.seek 1.04 Pucks below Top Plate @19:19:46.91 Forearm.reconfigure LooseForearm @19:19:46.94 Forearm.seek retract @19:19:47.86 Elbow.seek CC @19:19:49.39 Elbow.reconfigure LooseElbow @19:19:49.48 Forearm.seek CC @19:19:50.40 CC.seek sealed @19:19:51.33 Hand.grip @19:19:52.04 CC.seek open @19:19:55.43 Forearm.seek retract @19:19:56.37 Elbow.reconfigure TightElbow @19:19:56.41 Elbow.seek 1 @19:19:58.01 Elbow.reconfigure LimpElbow @19:19:58.06 Forearm.seek 1 @19:19:59.03 Elbow.hold @19:19:59.09 Elbow.reconfigure TightElbow @19:19:59.13 Hand.release @19:19:59.99 Forearm.coast @19:20:00.02 Forearm.seek 1 + 333 counts @19:20:00.25 Forearm.reconfigure TightForearm @19:20:00.28 Forearm.seek 1 + 800 counts @19:20:00.56 SE.seek down @19:20:01.66
Forearm.reconfigure LooseForearm @19:20:01.70 Forearm.seek retract @19:20:03.19 SE.coast @19:20:03.22
SC.coast @19:20:03.24 PSV.dial :air @19:20:04.31 PS.seek 0.499ml @19:20:10.24 delay 3 seconds @19:20:13.25 PSV.dial :mixloop @19:20:14.10 PRV.dial :air @19:20:14.54 delay 3 seconds @19:20:17.55 PRV.dial CBV @19:20:17.99 CBV.dial AIV @19:20:18.45 PRV.dial between CBV and air @19:20:18.76 PRV.dial :air @19:20:19.11 PS.reconfigure PSslow @19:20:19.14 PS.seek 0.599ml @19:20:21.65 PRV.dial :waste2 @19:20:22.38 PS.reconfigure PSconfig @19:20:22.41 PS.seek empty @19:20:29.43 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @19:20:29.58 Elbow.seek FlushPuck::Garage @19:20:31.33 Elbow.reconfigure LooseElbow @19:20:31.42 Forearm.seek garage @19:20:32.45 Hand.grip @19:20:33.25 Forearm.seek retract @19:20:34.24 Elbow.reconfigure TightElbow @19:20:34.28 Elbow.seek CC @19:20:37.03 Elbow.reconfigure LooseElbow @19:20:37.12 Forearm.reconfigure TightForearm @19:20:37.15 Forearm.seek CC @19:20:38.32 CC.seek closed @19:20:42.13 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 2.01 @19:20:42.18 Hand.release @19:20:42.95 Forearm.reconfigure LooseForearm @19:20:42.98 Forearm.seek retract @19:20:44.03 Clearing PML for modified lysate @19:20:44.04 CTV.dial :air,avoiding => intake @19:20:44.05 PRV.dial :waste2 @19:20:44.06 PTV.dial :PRV,avoiding => puck @19:20:44.78 delay 2 seconds @19:20:46.78 PTV.dial :PRVmixing,avoiding => puck @19:20:47.51 delay 2 seconds @19:20:49.52 PSV.dial :PML @19:20:49.53 delay 2 seconds @19:20:51.53 PRV.dial :air @19:20:52.27 delay 2 seconds @19:20:54.32 PS.seek 1.499ml @19:21:10.43 delay 5 seconds @19:21:15.44 PS.seek 2.500ml @19:21:26.43 PSV.dial :air @19:21:26.89 PS.seek 0.000ml @19:21:53.18 mixing lysate with speDil @19:21:53.19 PSV.dial :PML @19:21:53.65 CBV.dial :PRV @19:21:53.66 PRV.dial :air @19:21:53.67 delay 2 seconds @19:21:55.68 PRV.dial :speDil @19:21:56.11 delay 2 seconds @19:21:58.17 PS.reconfigure PSslow1 @19:21:58.20 PS.seek 0.225ml @19:22:21.52 delay 5 seconds @19:22:26.53 PRV.dial CBV @19:22:27.28 delay 2 seconds @19:22:29.33 PS.seek 0.475ml @19:22:55.02 delay 5 seconds @19:23:00.03 PRV.dial :air @19:23:00.47 delay 2 seconds @19:23:02.48 PS.seek 0.925ml @19:23:13.92 GoodNews.email "Then, in tube 2: wqPCR\n/home/esp/esp2/mission/14mar02sccwrp.rb:47",Subject => "Asleep until 18:28" @19:23:13.94 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @19:23:13.95 Retry #2 of 10 after 5:00 @19:23:48.55
delay 10 seconds @19:23:58.56 PRV.dial CBV @19:23:59.02 CTV.dial :air,avoiding => intake @19:23:59.03 PSV.dial :air @19:23:59.49 PS.reconfigure PSconfig @19:23:59.52 PS.seek 2.500ml @19:24:16.30 delay 3 seconds @19:24:19.31 PSV.dial PTV @19:24:20.05 PTV.dial :PRV,avoiding => puck @19:24:20.79 PS.seek 0.000ml @19:24:47.11 PSV.dial :air @19:24:47.85 PS.seek 2.500ml @19:25:14.05 delay 3 seconds @19:25:17.06 PSV.dial PTV @19:25:17.79 PTV.dial :PRV,avoiding => puck @19:25:17.81 PS.seek 0.000ml @19:25:44.02 Pushing modified lysate into AIV Injection Loop @19:25:44.03 CBV.dial AIV @19:25:44.04 AIV.dial :bypass @19:25:44.05 PRV.dial CBV @19:25:44.06 PSV.dial :air @19:25:44.80 PS.seek 2.500ml @19:26:10.99 delay 3 seconds @19:26:14.00 PSV.dial :PML @19:26:14.57 PTV.dial :PRVmixing,avoiding => puck @19:26:15.35 PS.reconfigure PSslow1 @19:26:15.38 PS.seek 1.850ml @19:27:21.83 delay 5 seconds @19:27:26.84 AIV.dial :sample @19:27:27.36 AWV.select :waste @19:27:27.52 PS.reconfigure PSslow2 @19:27:27.55 PS.seek 1.310ml @19:28:13.96 GoodNews.email "Then, in tube 2: wqPCR\n/home/esp/esp2/mission/14mar02sccwrp.rb:47",Subject => "Asleep until 18:28" @19:28:13.98 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @19:28:13.99 Retry #3 of 10 after 5:00 @19:29:17.70
delay 15 seconds @19:29:32.71 AIV.dial :bypass @19:29:33.26 PS.reconfigure PSconfig @19:29:33.29 PS.seek 0.000ml @19:29:47.42 PSV.dial :air @19:29:47.97 PS.seek 2.500ml @19:30:14.26 PSV.dial :PML @19:30:14.69 PS.seek 0.000ml @19:30:40.98 AIV.dial :coilToMFB @19:30:40.99 AWV.select :air @19:30:41.13 Extracting Nucleic Acid from AIV @19:30:41.15 SPE.hold 2:46:40,55.0C @19:30:41.17
Beginning samplePassD @19:30:41.19 ASV.dial :SPEdelivery @19:30:41.30
AWV.select :air @19:30:41.31 AIV.dial :bypass @19:30:41.32 PSV.dial PSR @19:30:41.69 delay 20 seconds @19:30:42.35
PSR.select :killMFB @19:30:42.45 PS.seek 1.500ml @19:30:58.51 delay 5 seconds @19:31:01.70 primeLysateINT @19:31:01.71 AWV.select :air @19:31:01.72 AIV.dial :coilToMFB @19:31:01.73 ATV.dial :AIV @19:31:03.14 AS.reconfigure ASslow1 @19:31:03.17 AS.seek 0.239ml @19:31:03.52
PSV.dial :waste1 @19:31:03.97 PS.seek 0.000ml @19:31:20.04 PSV.dial PSR @19:31:21.01 PSR.select :air @19:31:21.11 PS.seek 2.500ml @19:31:47.32 PRV.dial CBV @19:31:47.34
CBV.dial PRV @19:31:47.35 PSV.dial :mixloop @19:31:48.39 CTV.dial :air,avoiding => intake @19:31:48.40 PTV.dial :PRVmixing @19:31:48.41 PS.seek 0.000ml @19:31:52.39 delay 10 seconds @19:32:02.40 ATV.dial :air2 @19:32:03.09 AS.seek 0.269ml @19:32:09.45 ATV.dial :waste2 @19:32:09.90 AS.reconfigure ASnorm @19:32:09.93 AS.seek empty @19:32:14.69
PSV.dial PSR @19:32:15.72 PSR.select :flush @19:32:15.82 PS.seek 1.500ml @19:32:24.11 Lysate primed! @19:32:24.12 AFV.select :holdingCoil @19:32:24.13 ATV.dial :air2 @19:32:24.50 AS.reconfigure ASslow1 @19:32:24.54 AS.seek 0.069ml @19:32:31.85
delay 5 seconds @19:32:36.86 PSV.dial :waste1 @19:32:37.31 PS.seek 0.000ml @19:32:39.12 delay 3 seconds @19:32:42.13 ATV.dial :AIV @19:32:42.69 AS.seek 0.469ml @19:32:53.38
PSV.dial PSR @19:32:53.83 PSR.select :air @19:32:53.93 PS.seek 2.500ml @19:33:13.99 GoodNews.email "Then, in tube 2: wqPCR\n/home/esp/esp2/mission/14mar02sccwrp.rb:47",Subject => "Asleep until 18:28" @19:33:14.01 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @19:33:14.02 Retry #4 of 10 after 5:00 @19:33:20.16 PRV.dial CBV @19:33:20.18
CBV.dial PRV @19:33:20.19 PSV.dial :mixloop @19:33:21.20 CTV.dial :air,avoiding => intake @19:33:21.21 PTV.dial :PRVmixing @19:33:21.23 PS.seek 0.000ml @19:33:47.53 PSV.dial PSR @19:33:48.55 PSR.select :flush @19:33:48.65 PS.seek 1.500ml @19:34:04.51 delay 5 seconds @19:34:04.75
delay 5 seconds @19:34:09.56 Solid Phase Extraction Heater at 54.0C @19:34:09.57 ATV.dial ASV @19:34:09.76
PSV.dial :waste1 @19:34:10.01 ASV.dial :SPEdelivery @19:34:10.07 AS.seek 0.041ml @19:34:10.21
PS.seek 0.000ml @19:34:26.34 PSV.dial PSR @19:34:26.80 PSR.select :air @19:34:26.90 PS.seek 2.500ml @19:34:53.18 PRV.dial CBV @19:34:53.20
CBV.dial PRV @19:34:53.21 PSV.dial :mixloop @19:34:54.22 CTV.dial :air,avoiding => intake @19:34:54.23 PTV.dial :PRVmixing @19:34:54.25 PS.seek 0.000ml @19:35:20.47 PSV.dial PSR @19:35:21.61 PSR.select :flush @19:35:21.71 PS.seek 1.500ml @19:35:37.62 delay 10 seconds @19:35:37.81
delay 5 seconds @19:35:42.82 PSV.dial :waste1 @19:35:43.29 PS.seek 0.000ml @19:35:47.63 rinseLysateLineINT @19:35:47.64 AFV.select :carrierSolution @19:35:47.73 delay 2 seconds @19:35:49.74 AS.reconfigure ASconfig @19:35:49.77 AS.seek 1.000ml @19:35:59.40
PSV.dial PSR @19:35:59.85 PSR.select :air @19:35:59.95 PS.seek 2.500ml @19:36:26.21 PRV.dial CBV @19:36:26.23
CBV.dial PRV @19:36:26.24 PSV.dial :mixloop @19:36:27.24 CTV.dial :air,avoiding => intake @19:36:27.25 PTV.dial :PRVmixing @19:36:27.26 PS.reconfigure PSslow @19:36:27.30 PS.seek 1.200ml @19:36:45.91 delay 1 second @19:36:46.92 AFV.select :holdingCoil @19:36:47.01 delay 1 second @19:36:48.02 ATV.dial :AIV @19:36:48.38 AIV.dial :coilToMFB @19:36:48.39 AWV.select :waste @19:36:48.49 AS.reconfigure ASslow0 @19:36:48.53 AS.seek 0.000ml @19:36:54.27
PSV.dial :waste1 @19:36:55.58 PS.reconfigure PSconfig @19:36:55.61 PS.seek 0.000ml @19:37:08.80 PSV.dial :air @19:37:09.83 PS.seek 2.500ml @19:37:36.02 PSV.dial :mixloop @19:37:36.47 PS.reconfigure PSslow @19:37:36.50 PS.seek 0.000ml @19:38:14.02 GoodNews.email "Then, in tube 2: wqPCR\n/home/esp/esp2/mission/14mar02sccwrp.rb:47",Subject => "Asleep until 18:28" @19:38:14.04 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @19:38:14.05 Retry #5 of 10 after 5:00 @19:38:27.86
PSV.dial :air @19:38:28.31 PS.reconfigure PSconfig @19:38:28.34 PS.seek 2.500ml @19:38:30.86 ATV.dial :air2 @19:38:31.52 AS.reconfigure ASslow1 @19:38:31.55 AS.seek 0.050ml @19:38:42.06 ATV.dial :AIV @19:38:42.72 AS.seek 0.010ml @19:38:51.13 ATV.dial :waste @19:38:52.40 AS.seek empty @19:38:54.61
PSV.dial :mixloop @19:38:55.36 ethanol wash @19:38:55.37 ATV.dial :air2 @19:38:55.41
PS.reconfigure PSslow @19:38:55.44 PS.seek 0.000ml @19:38:56.30 AS.reconfigure ASslow2 @19:38:56.33 AS.seek 0.009ml @19:39:07.11 delay 3 seconds @19:39:10.12 Pulling 0.4ml of Ethanol Wash @19:39:10.13 ATV.dial :ethanol @19:39:10.69 AS.reconfigure ASslow1 @19:39:10.72 AS.seek 0.409ml @19:39:46.77
Cleaning Collection Syringe @19:39:46.78 CSV.dial CSR @19:39:47.20 CSR.select :flush @19:39:47.30 CS.seek 4.000ml @19:39:58.25 delay 5 seconds @19:40:03.26 CSR.select :air @19:40:03.35 CS.seek 10.000ml @19:40:19.40 CSV.dial CTV @19:40:19.42
CTV.dial CSV @19:40:20.75 CBV.dial :waste1 @19:40:21.24 CS.seek 0.000ml @19:40:32.56 delay 3 seconds @19:40:35.57 ATV.dial :air1 @19:40:35.90 delay 3 seconds @19:40:38.96 AS.seek 0.434ml @19:40:44.29 delay 3 seconds @19:40:47.30 ATV.dial :ethanol @19:40:47.43
CSV.dial CSR @19:40:47.67 AS.seek 0.419ml @19:40:47.93
CSR.select :flush @19:40:48.03 CS.seek 4.000ml @19:40:50.98 delay 3 seconds @19:40:53.99 ATV.dial ASV @19:40:55.11 AS.seek 0.092ml @19:40:59.03
delay 5 seconds @19:41:04.04 CSR.select :air @19:41:04.13 CS.seek 10.000ml @19:41:20.06 CSV.dial CTV @19:41:20.08
CTV.dial CSV @19:41:20.60 CBV.dial :waste1 @19:41:20.61 CS.seek 0.000ml @19:41:46.92 Garaging FlushPuck in Collection Clamp @19:41:46.98 Elbow.reconfigure TightElbow @19:41:47.01 Elbow.seek CC @19:41:47.11 Elbow.reconfigure LooseElbow @19:41:47.19 Forearm.seek CC @19:41:48.19 CC.seek sealed @19:41:49.12 Hand.grip @19:41:49.86 CC.seek open @19:41:53.22 Forearm.seek retract @19:41:54.21 Elbow.reconfigure TightElbow @19:41:54.25 Elbow.seek garage @19:41:57.04 Elbow.reconfigure LooseElbow @19:41:57.12 Forearm.reconfigure TightForearm @19:41:57.15 Forearm.seek garage @19:41:58.32 Hand.release @19:41:59.15 Forearm.reconfigure LooseForearm @19:41:59.18 Forearm.seek retract @19:42:00.21 Whole Cell Archive sampling 1000ml @19:42:00.23 Loading wcr puck from tube 2 into Collection Clamp @19:42:00.40 SC.reconfigure SCtight @19:42:00.44 SC.seek 2 @19:42:02.06 delay 3 seconds @19:42:02.68 SC.reconfigure SCloose @19:42:02.86 Elbow.reconfigure TightElbow @19:42:02.89 Elbow.seek 2 @19:42:04.73 Forearm.reconfigure TightForearm @19:42:04.76 Forearm.seek 2 + 800 counts @19:42:05.07 ATV.dial :waste2 @19:42:05.71 AS.reconfigure ASconfig @19:42:05.74 AS.seek empty @19:42:05.88 SE.reconfigure SEpick @19:42:05.94 SE.seek beamBreak @19:42:07.00 SE.configure SEconfig @19:42:07.14 Elbow.seek 2 @19:42:07.29 Forearm.seek 2 + 300 counts @19:42:07.69 Forearm.reconfigure LooseForearm @19:42:07.73 Hand.grip @19:42:08.50 SE.seek down @19:42:08.63 Forearm.seek retract @19:42:09.37 SE.coast @19:42:09.58 Elbow.seek CC @19:42:11.46 ATV.dial :air2 @19:42:11.63 Elbow.reconfigure LooseElbow @19:42:11.71 Forearm.reconfigure TightForearm @19:42:11.74 Forearm.seek CC @19:42:11.83 AS.reconfigure ASslow1 @19:42:11.86 AS.seek 1.000ml @19:42:12.87 CC.seek closed @19:42:16.64 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 2.03 @19:42:16.65 Hand.release @19:42:17.41 Forearm.reconfigure LooseForearm @19:42:17.45 Forearm.seek retract @19:42:18.42 SC.coast @19:42:18.45 collectSample 1000ml w/bubble point of 55psi @19:42:18.47 Priming 7ml Intake.select :close @19:42:18.48 Exhaust.select :close @19:42:18.49 SS.hold @19:42:18.64 Calibrating pressure sensors @19:42:18.65 CTV.dial :air @19:42:19.92 SSV.dial :spit,avoiding => exhaust @19:42:19.93 SS.reconfigure SSfast @19:42:19.96 SS.seek 5.00ml @19:42:22.63 SSV.dial CBV @19:42:22.64 CBV.dial SSV @19:42:23.99 delay 3.8 seconds @19:42:27.88 SSV.dial :spit @19:42:29.18 SS.seek 0.00ml @19:42:31.73 SSV.dial CBV @19:42:33.01 CTV.dial :intake @19:42:33.73 CBV.dial SSV @19:42:33.74 SSV.dial CBV @19:42:33.76 Allowing 55s for 7.0ml of remaining 7.0ml @19:42:33.77 Intake.select :open @19:42:35.91 Intake @33.5psia, vacuum from 20.0 to 24.0psi @19:42:35.93 SS.reconfigure forSampling @19:42:35.96 SS.seek 7.00ml @19:42:41.69 Stroke completed -- waiting for pressure to equalize ... @19:42:42.33 Intake.select :close @19:42:42.47 SSV.dial :exhaust @19:42:43.23 Exhaust.select :open @19:42:43.34 SS.reconfigure SSexhale @19:42:43.38 SS.seek 0.00ml @19:42:46.87 Exhaust.select :close @19:42:46.97 Sampling 1000.0ml @19:42:46.98 Intake.select :close @19:42:46.99 Exhaust.select :close @19:42:47.00 SS.reconfigure SSconfig @19:42:47.03 SS.hold @19:42:47.19 CTV.dial :intake @19:42:47.20 SSV.dial CBV @19:42:47.22 CBV.dial SSV @19:42:48.01 Allowing 185s for 25.0ml of remaining 1000.0ml @19:42:48.02 Intake.select :open @19:42:50.15 Intake @33.4psia, vacuum from 20.0 to 24.0psi @19:42:50.17 SS.reconfigure forSampling @19:42:50.20 SS.seek 25.00ml @19:43:04.29 Stroke completed -- waiting for pressure to equalize ... @19:43:04.93 Intake.select :close @19:43:05.07 SSV.dial :exhaust @19:43:05.83 Exhaust.select :open @19:43:05.93 SS.reconfigure SSexhale @19:43:05.97 SS.seek 0.00ml @19:43:14.06 GoodNews.email "Then, in tube 2: wqPCR\n/home/esp/esp2/mission/14mar02sccwrp.rb:47",Subject => "Asleep until 18:28" @19:43:14.08 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @19:43:14.09 Retry #6 of 10 after 5:00 @19:43:14.81 Exhaust.select :close @19:43:14.91 SSV.dial CBV @19:43:14.93 CBV.dial SSV @19:43:15.70 Allowing 185s for 25.0ml of remaining 975.0ml @19:43:15.72 Intake.select :open @19:43:17.85 Intake @33.3psia, vacuum from 20.0 to 24.0psi @19:43:17.87 SS.reconfigure forSampling @19:43:17.90 SS.seek 25.00ml @19:43:32.15 Stroke completed -- waiting for pressure to equalize ... @19:43:32.79 Intake.select :close @19:43:32.93 SSV.dial :exhaust @19:43:33.68 Exhaust.select :open @19:43:33.78 SS.reconfigure SSexhale @19:43:33.82 SS.seek 0.00ml @19:43:42.48 Exhaust.select :close @19:43:42.58 SSV.dial CBV @19:43:42.60 CBV.dial SSV @19:43:43.35 Allowing 185s for 25.0ml of remaining 950.0ml @19:43:43.37 Intake.select :open @19:43:45.50 Intake @33.4psia, vacuum from 20.0 to 24.0psi @19:43:45.52 SS.reconfigure forSampling @19:43:45.55 SS.seek 25.00ml @19:43:59.73 Stroke completed -- waiting for pressure to equalize ... @19:44:00.37 Intake.select :close @19:44:00.51 SSV.dial :exhaust @19:44:01.26 Exhaust.select :open @19:44:01.87 SS.reconfigure SSexhale @19:44:01.91 SS.seek 0.00ml @19:44:10.43 Exhaust.select :close @19:44:10.53 SSV.dial CBV @19:44:10.55 CBV.dial SSV @19:44:11.33 Allowing 185s for 25.0ml of remaining 925.0ml @19:44:11.34 Intake.select :open @19:44:13.47 Intake @33.5psia, vacuum from 20.0 to 24.0psi @19:44:13.49 SS.reconfigure forSampling @19:44:13.52 SS.seek 25.00ml @19:44:27.73 Stroke completed -- waiting for pressure to equalize ... @19:44:28.38 Intake.select :close @19:44:28.51 SSV.dial :exhaust @19:44:29.27 Exhaust.select :open @19:44:29.38 SS.reconfigure SSexhale @19:44:29.41 SS.seek 0.00ml @19:44:37.91 Exhaust.select :close @19:44:38.01 SSV.dial CBV @19:44:38.03 CBV.dial SSV @19:44:38.80 Allowing 185s for 25.0ml of remaining 900.0ml @19:44:38.81 Intake.select :open @19:44:40.94 Intake @33.5psia, vacuum from 20.0 to 24.0psi @19:44:40.96 SS.reconfigure forSampling @19:44:40.99 SS.seek 25.00ml @19:44:55.18 Stroke completed -- waiting for pressure to equalize ... @19:44:55.82 Intake.select :close @19:44:55.96 SSV.dial :exhaust @19:44:56.71 Exhaust.select :open @19:44:56.81 SS.reconfigure SSexhale @19:44:56.85 SS.seek 0.00ml @19:45:05.40 Exhaust.select :close @19:45:05.50 SSV.dial CBV @19:45:05.52 CBV.dial SSV @19:45:06.30 Allowing 185s for 25.0ml of remaining 875.0ml @19:45:06.31 Intake.select :open @19:45:08.44 Intake @33.4psia, vacuum from 20.0 to 24.0psi @19:45:08.46 SS.reconfigure forSampling @19:45:08.49 SS.seek 25.00ml @19:45:22.74 Stroke completed -- waiting for pressure to equalize ... @19:45:23.38 Intake.select :close @19:45:23.52 SSV.dial :exhaust @19:45:24.27 Exhaust.select :open @19:45:24.37 SS.reconfigure SSexhale @19:45:24.41 SS.seek 0.00ml @19:45:33.47 Exhaust.select :close @19:45:33.57 SSV.dial CBV @19:45:33.59 CBV.dial SSV @19:45:34.36 Allowing 185s for 25.0ml of remaining 850.0ml @19:45:34.37 Intake.select :open @19:45:36.28 delay 5 seconds @19:45:36.51 Intake @33.7psia, vacuum from 20.0 to 24.0psi @19:45:36.53 SS.reconfigure forSampling @19:45:36.56 SS.seek 25.00ml @19:45:41.29 ATV.dial ASV @19:45:42.01 ASV.dial :SPEdelivery @19:45:42.03 AS.seek 0.020ml @19:45:50.61 Stroke completed -- waiting for pressure to equalize ... @19:45:51.25 Intake.select :close @19:45:51.39 SSV.dial :exhaust @19:45:52.14 Exhaust.select :open @19:45:52.24 SS.reconfigure SSexhale @19:45:52.28 SS.seek 0.00ml @19:46:01.03 Exhaust.select :close @19:46:01.13 SSV.dial CBV @19:46:01.15 CBV.dial SSV @19:46:01.91 Allowing 185s for 25.0ml of remaining 825.0ml @19:46:01.93 Intake.select :open @19:46:04.06 Intake @33.7psia, vacuum from 20.0 to 24.0psi @19:46:04.08 SS.reconfigure forSampling @19:46:04.11 SS.seek 25.00ml @19:46:18.25 Stroke completed -- waiting for pressure to equalize ... @19:46:18.89 Intake.select :close @19:46:19.03 SSV.dial :exhaust @19:46:19.78 Exhaust.select :open @19:46:19.88 SS.reconfigure SSexhale @19:46:19.92 SS.seek 0.00ml @19:46:28.45 Exhaust.select :close @19:46:28.55 SSV.dial CBV @19:46:28.57 CBV.dial SSV @19:46:29.35 Allowing 185s for 25.0ml of remaining 800.0ml @19:46:29.36 Intake.select :open @19:46:31.50 Intake @33.7psia, vacuum from 20.0 to 24.0psi @19:46:31.52 SS.reconfigure forSampling @19:46:31.55 SS.seek 25.00ml @19:46:45.30 Stroke completed -- waiting for pressure to equalize ... @19:46:45.94 Intake.select :close @19:46:46.08 SSV.dial :exhaust @19:46:46.83 Exhaust.select :open @19:46:46.94 SS.reconfigure SSexhale @19:46:46.98 SS.seek 0.00ml @19:46:55.31 Exhaust.select :close @19:46:55.41 SSV.dial CBV @19:46:55.43 CBV.dial SSV @19:46:56.19 Allowing 185s for 25.0ml of remaining 775.0ml @19:46:56.21 Intake.select :open @19:46:58.74 Intake @33.6psia, vacuum from 20.0 to 24.0psi @19:46:58.76 SS.reconfigure forSampling @19:46:58.79 SS.seek 25.00ml @19:47:13.36 Stroke completed -- waiting for pressure to equalize ... @19:47:14.01 Intake.select :close @19:47:14.15 SSV.dial :exhaust @19:47:14.91 Exhaust.select :open @19:47:15.02 SS.reconfigure SSexhale @19:47:15.55 SS.seek 0.00ml @19:47:24.06 Exhaust.select :close @19:47:24.16 SSV.dial CBV @19:47:24.18 CBV.dial SSV @19:47:24.95 Allowing 185s for 25.0ml of remaining 750.0ml @19:47:24.96 Intake.select :open @19:47:27.09 Intake @33.7psia, vacuum from 20.0 to 24.0psi @19:47:27.11 SS.reconfigure forSampling @19:47:27.14 SS.seek 25.00ml @19:47:41.44 Stroke completed -- waiting for pressure to equalize ... @19:47:42.08 Intake.select :close @19:47:42.22 SSV.dial :exhaust @19:47:42.98 Exhaust.select :open @19:47:43.09 SS.reconfigure SSexhale @19:47:43.12 SS.seek 0.00ml @19:47:51.67 Exhaust.select :close @19:47:51.77 SSV.dial CBV @19:47:51.79 CBV.dial SSV @19:47:52.56 Allowing 185s for 25.0ml of remaining 725.0ml @19:47:52.58 Intake.select :open @19:47:54.71 Intake @33.7psia, vacuum from 20.0 to 24.0psi @19:47:54.73 SS.reconfigure forSampling @19:47:54.76 SS.seek 25.00ml @19:48:08.92 Stroke completed -- waiting for pressure to equalize ... @19:48:09.57 Intake.select :close @19:48:09.70 SSV.dial :exhaust @19:48:10.43 Exhaust.select :open @19:48:10.54 SS.reconfigure SSexhale @19:48:10.58 SS.seek 0.00ml @19:48:14.09 GoodNews.email "Then, in tube 2: wqPCR\n/home/esp/esp2/mission/14mar02sccwrp.rb:47",Subject => "Asleep until 18:28" @19:48:14.11 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @19:48:14.12 Retry #7 of 10 after 5:00 @19:48:19.01 Exhaust.select :close @19:48:19.11 SSV.dial CBV @19:48:19.13 CBV.dial SSV @19:48:19.89 Allowing 185s for 25.0ml of remaining 700.0ml @19:48:19.91 Intake.select :open @19:48:22.04 Intake @33.8psia, vacuum from 20.0 to 24.0psi @19:48:22.06 SS.reconfigure forSampling @19:48:22.09 SS.seek 25.00ml @19:48:36.20 Stroke completed -- waiting for pressure to equalize ... @19:48:36.84 Intake.select :close @19:48:36.98 SSV.dial :exhaust @19:48:37.71 Exhaust.select :open @19:48:37.81 SS.reconfigure SSexhale @19:48:37.85 SS.seek 0.00ml @19:48:46.29 Exhaust.select :close @19:48:46.39 SSV.dial CBV @19:48:46.41 CBV.dial SSV @19:48:47.17 Allowing 185s for 25.0ml of remaining 675.0ml @19:48:47.19 Intake.select :open @19:48:49.32 Intake @33.8psia, vacuum from 20.0 to 24.0psi @19:48:49.34 SS.reconfigure forSampling @19:48:49.37 SS.seek 25.00ml @19:49:02.24 delay 5 seconds @19:49:03.18 Stroke completed -- waiting for pressure to equalize ... @19:49:03.82 Intake.select :close @19:49:03.96 SSV.dial :exhaust @19:49:04.70 Exhaust.select :open @19:49:04.80 SS.reconfigure SSexhale @19:49:04.84 SS.seek 0.00ml @19:49:07.25 ATV.dial :air2 @19:49:08.13 AS.seek 0.520ml @19:49:13.43 Exhaust.select :close @19:49:13.53 SSV.dial CBV @19:49:13.55 CBV.dial SSV @19:49:14.32 Allowing 185s for 25.0ml of remaining 650.0ml @19:49:14.33 Intake.select :open @19:49:16.46 Intake @33.9psia, vacuum from 20.0 to 24.0psi @19:49:16.48 SS.reconfigure forSampling @19:49:16.51 SS.seek 25.00ml @19:49:30.29 Stroke completed -- waiting for pressure to equalize ... @19:49:30.93 Intake.select :close @19:49:31.07 SSV.dial :exhaust @19:49:31.80 Exhaust.select :open @19:49:31.90 SS.reconfigure SSexhale @19:49:31.94 SS.seek 0.00ml @19:49:40.76 Exhaust.select :close @19:49:40.86 SSV.dial CBV @19:49:40.88 CBV.dial SSV @19:49:41.64 Allowing 185s for 25.0ml of remaining 625.0ml @19:49:41.65 Intake.select :open @19:49:43.78 Intake @33.8psia, vacuum from 20.0 to 24.0psi @19:49:43.80 SS.reconfigure forSampling @19:49:43.83 SS.seek 25.00ml @19:49:57.82 Stroke completed -- waiting for pressure to equalize ... @19:49:58.46 Intake.select :close @19:49:58.60 SSV.dial :exhaust @19:49:59.33 Exhaust.select :open @19:49:59.43 SS.reconfigure SSexhale @19:49:59.47 SS.seek 0.00ml @19:50:08.23 Exhaust.select :close @19:50:08.33 SSV.dial CBV @19:50:08.35 CBV.dial SSV @19:50:09.10 Allowing 185s for 25.0ml of remaining 600.0ml @19:50:09.12 Intake.select :open @19:50:11.25 Intake @33.7psia, vacuum from 20.0 to 24.0psi @19:50:11.27 SS.reconfigure forSampling @19:50:11.30 SS.seek 25.00ml @19:50:25.43 Stroke completed -- waiting for pressure to equalize ... @19:50:26.07 Intake.select :close @19:50:26.21 SSV.dial :exhaust @19:50:26.96 Exhaust.select :open @19:50:27.54 SS.reconfigure SSexhale @19:50:27.58 SS.seek 0.00ml @19:50:36.27 Exhaust.select :close @19:50:36.37 SSV.dial CBV @19:50:36.39 CBV.dial SSV @19:50:37.15 Allowing 185s for 25.0ml of remaining 575.0ml @19:50:37.16 Intake.select :open @19:50:39.29 Intake @33.9psia, vacuum from 20.0 to 24.0psi @19:50:39.31 SS.reconfigure forSampling @19:50:39.34 SS.seek 25.00ml @19:50:50.36 delay 5 seconds @19:50:53.02 Stroke completed -- waiting for pressure to equalize ... @19:50:53.66 Intake.select :close @19:50:53.80 SSV.dial :exhaust @19:50:54.53 Exhaust.select :open @19:50:54.63 SS.reconfigure SSexhale @19:50:54.67 SS.seek 0.00ml @19:50:55.37 ATV.dial ASV @19:50:56.17 ASV.dial :wasteR @19:50:56.70 AS.seek 0.300ml @19:51:03.42 Exhaust.select :close @19:51:03.52 SSV.dial CBV @19:51:03.54 CBV.dial SSV @19:51:04.30 Allowing 185s for 25.0ml of remaining 550.0ml @19:51:04.32 Intake.select :open @19:51:06.45 Intake @33.7psia, vacuum from 20.0 to 24.0psi @19:51:06.47 SS.reconfigure forSampling @19:51:06.50 SS.seek 25.00ml @19:51:20.24 Stroke completed -- waiting for pressure to equalize ... @19:51:20.88 Intake.select :close @19:51:21.02 SSV.dial :exhaust @19:51:21.77 Exhaust.select :open @19:51:21.87 SS.reconfigure SSexhale @19:51:21.91 SS.seek 0.00ml @19:51:30.45 Exhaust.select :close @19:51:30.55 SSV.dial CBV @19:51:30.57 CBV.dial SSV @19:51:31.33 Allowing 185s for 25.0ml of remaining 525.0ml @19:51:31.34 Intake.select :open @19:51:33.48 Intake @33.8psia, vacuum from 20.0 to 24.0psi @19:51:33.50 SS.reconfigure forSampling @19:51:33.53 SS.seek 25.00ml @19:51:41.83 delay 20 seconds @19:51:47.27 Stroke completed -- waiting for pressure to equalize ... @19:51:47.91 Intake.select :close @19:51:48.05 SSV.dial :exhaust @19:51:48.80 Exhaust.select :open @19:51:48.90 SS.reconfigure SSexhale @19:51:48.94 SS.seek 0.00ml @19:51:57.97 Exhaust.select :close @19:51:58.07 SSV.dial CBV @19:51:58.10 CBV.dial SSV @19:51:58.87 Allowing 185s for 25.0ml of remaining 500.0ml @19:51:58.88 Intake.select :open @19:52:01.01 Intake @33.8psia, vacuum from 20.0 to 24.0psi @19:52:01.03 SS.reconfigure forSampling @19:52:01.06 SS.seek 25.00ml @19:52:01.84 AS.seek 0.070ml @19:52:15.22 Stroke completed -- waiting for pressure to equalize ... @19:52:15.86 Intake.select :close @19:52:16.01 SSV.dial :exhaust @19:52:16.75 Exhaust.select :open @19:52:16.85 SS.reconfigure SSexhale @19:52:16.89 SS.seek 0.00ml @19:52:25.62 Exhaust.select :close @19:52:25.72 SSV.dial CBV @19:52:25.74 CBV.dial SSV @19:52:26.50 Allowing 185s for 25.0ml of remaining 475.0ml @19:52:26.52 Intake.select :open @19:52:28.65 Intake @33.8psia, vacuum from 20.0 to 24.0psi @19:52:28.67 SS.reconfigure forSampling @19:52:28.70 SS.seek 25.00ml @19:52:42.47 Stroke completed -- waiting for pressure to equalize ... @19:52:43.11 Intake.select :close @19:52:43.27 SSV.dial :exhaust @19:52:44.00 Exhaust.select :open @19:52:44.11 SS.reconfigure SSexhale @19:52:44.15 SS.seek 0.00ml @19:52:48.98 delay 20 seconds @19:52:52.59 Exhaust.select :close @19:52:52.69 SSV.dial CBV @19:52:52.71 CBV.dial SSV @19:52:53.47 Allowing 185s for 25.0ml of remaining 450.0ml @19:52:53.49 Intake.select :open @19:52:55.62 Intake @33.9psia, vacuum from 20.0 to 24.0psi @19:52:55.64 SS.reconfigure forSampling @19:52:55.67 SS.seek 25.00ml @19:53:08.99 ATV.dial :waste2 @19:53:09.42 Stroke completed -- waiting for pressure to equalize ... @19:53:09.62 AS.reconfigure ASconfig @19:53:09.65 AS.seek empty @19:53:10.06 Intake.select :close @19:53:10.20 SSV.dial :exhaust @19:53:10.93 Exhaust.select :open @19:53:11.03 SS.reconfigure SSexhale @19:53:11.07 SS.seek 0.00ml @19:53:14.07 Elution @19:53:14.08 ATV.dial :air1 @19:53:14.12 GoodNews.email "Then, in tube 2: wqPCR\n/home/esp/esp2/mission/14mar02sccwrp.rb:47",Subject => "Asleep until 18:28" @19:53:14.14 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @19:53:14.15 Retry #8 of 10 after 5:00 @19:53:15.01 AS.reconfigure ASslow1 @19:53:15.04 AS.seek 0.049ml @19:53:19.82 Exhaust.select :close @19:53:19.92 SSV.dial CBV @19:53:19.94 CBV.dial SSV @19:53:21.21 Allowing 185s for 25.0ml of remaining 425.0ml @19:53:21.22 Intake.select :open @19:53:23.35 Intake @33.9psia, vacuum from 20.0 to 24.0psi @19:53:23.37 SS.reconfigure forSampling @19:53:23.40 SS.seek 25.00ml @19:53:25.51 delay 3 seconds @19:53:28.52 ATV.dial :ElutionWater @19:53:29.08 AS.seek 0.109ml @19:53:37.59 Stroke completed -- waiting for pressure to equalize ... @19:53:38.23 Intake.select :close @19:53:38.37 SSV.dial :exhaust @19:53:39.10 Exhaust.select :open @19:53:39.20 SS.reconfigure SSexhale @19:53:39.24 SS.seek 0.00ml @19:53:41.59 delay 3 seconds @19:53:44.60 ATV.dial ASV,via => air2 @19:53:46.23 ASV.dial :SPEelution @19:53:47.07 AFV.select :carrierSolution @19:53:47.22 AS.reconfigure ASnorm @19:53:47.25 AS.seek 0.759ml @19:53:47.90 Exhaust.select :close @19:53:48.00 SSV.dial CBV @19:53:48.02 CBV.dial SSV @19:53:48.78 Allowing 185s for 25.0ml of remaining 400.0ml @19:53:48.79 Intake.select :open @19:53:50.92 Intake @33.8psia, vacuum from 20.0 to 24.0psi @19:53:50.94 SS.reconfigure forSampling @19:53:50.97 SS.seek 25.00ml @19:54:05.26 Stroke completed -- waiting for pressure to equalize ... @19:54:05.90 Intake.select :close @19:54:06.04 SSV.dial :exhaust @19:54:06.77 Exhaust.select :open @19:54:06.87 SS.reconfigure SSexhale @19:54:06.91 SS.seek 0.00ml @19:54:15.66 Exhaust.select :close @19:54:15.76 SSV.dial CBV @19:54:15.78 CBV.dial SSV @19:54:16.55 Allowing 185s for 25.0ml of remaining 375.0ml @19:54:16.56 Intake.select :open @19:54:18.69 Intake @34.0psia, vacuum from 20.0 to 24.0psi @19:54:18.71 SS.reconfigure forSampling @19:54:18.74 SS.seek 25.00ml @19:54:20.74 delay 3 seconds @19:54:23.75 AFV.select :holdingCoil @19:54:23.90 AS.reconfigure ASslow1 @19:54:23.93 AS.seek 0.489ml @19:54:32.88 Stroke completed -- waiting for pressure to equalize ... @19:54:33.52 Intake.select :close @19:54:33.66 SSV.dial :exhaust @19:54:34.40 Exhaust.select :open @19:54:34.50 SS.reconfigure SSexhale @19:54:34.54 SS.seek 0.00ml @19:54:43.08 Exhaust.select :close @19:54:43.18 SSV.dial CBV @19:54:43.20 CBV.dial SSV @19:54:43.96 Allowing 185s for 25.0ml of remaining 350.0ml @19:54:43.98 Intake.select :open @19:54:46.11 Intake @34.0psia, vacuum from 20.0 to 24.0psi @19:54:46.13 SS.reconfigure forSampling @19:54:46.16 SS.seek 25.00ml @19:54:59.93 Stroke completed -- waiting for pressure to equalize ... @19:55:00.57 Intake.select :close @19:55:00.71 SSV.dial :exhaust @19:55:01.46 Exhaust.select :open @19:55:01.56 SS.reconfigure SSexhale @19:55:01.60 SS.seek 0.00ml @19:55:10.46 Exhaust.select :close @19:55:10.56 SSV.dial CBV @19:55:10.58 CBV.dial SSV @19:55:11.33 Allowing 185s for 25.0ml of remaining 325.0ml @19:55:11.35 Intake.select :open @19:55:13.48 Intake @34.0psia, vacuum from 20.0 to 24.0psi @19:55:13.50 SS.reconfigure forSampling @19:55:13.53 SS.seek 25.00ml @19:55:19.24 delay 5 seconds @19:55:24.30 AS.reconfigure ASslow2 @19:55:24.33 AS.seek 0.454ml @19:55:27.74 Stroke completed -- waiting for pressure to equalize ... @19:55:28.38 Intake.select :close @19:55:28.52 SSV.dial :exhaust @19:55:29.25 Exhaust.select :open @19:55:29.35 SS.reconfigure SSexhale @19:55:29.39 SS.seek 0.00ml @19:55:37.96 Exhaust.select :close @19:55:38.06 SSV.dial CBV @19:55:38.08 CBV.dial SSV @19:55:38.85 Allowing 185s for 25.0ml of remaining 300.0ml @19:55:38.87 Intake.select :open @19:55:41.01 Intake @34.0psia, vacuum from 20.0 to 24.0psi @19:55:41.02 SS.reconfigure forSampling @19:55:41.05 SS.seek 25.00ml @19:55:55.19 Stroke completed -- waiting for pressure to equalize ... @19:55:55.83 Intake.select :close @19:55:55.97 SSV.dial :exhaust @19:55:56.71 Exhaust.select :open @19:55:56.82 SS.reconfigure SSexhale @19:55:56.86 SS.seek 0.00ml @19:56:00.24 delay 1:00 @19:56:05.42 Exhaust.select :close @19:56:05.52 SSV.dial CBV @19:56:05.54 CBV.dial SSV @19:56:06.30 Allowing 185s for 25.0ml of remaining 275.0ml @19:56:06.31 Intake.select :open @19:56:08.45 Intake @33.9psia, vacuum from 20.0 to 24.0psi @19:56:08.47 SS.reconfigure forSampling @19:56:08.50 SS.seek 25.00ml @19:56:23.22 Stroke completed -- waiting for pressure to equalize ... @19:56:23.87 Intake.select :close @19:56:24.00 SSV.dial :exhaust @19:56:24.73 Exhaust.select :open @19:56:24.84 SS.reconfigure SSexhale @19:56:24.88 SS.seek 0.00ml @19:56:33.66 Exhaust.select :close @19:56:33.76 SSV.dial CBV @19:56:33.78 CBV.dial SSV @19:56:34.54 Allowing 185s for 25.0ml of remaining 250.0ml @19:56:34.55 Intake.select :open @19:56:36.68 Intake @34.0psia, vacuum from 20.0 to 24.0psi @19:56:36.70 SS.reconfigure forSampling @19:56:36.73 SS.seek 25.00ml @19:56:50.81 Stroke completed -- waiting for pressure to equalize ... @19:56:51.45 Intake.select :close @19:56:51.60 SSV.dial :exhaust @19:56:52.33 Exhaust.select :open @19:56:52.43 SS.reconfigure SSexhale @19:56:52.47 SS.seek 0.00ml @19:57:00.25 pushing eluate to holding coil @19:57:00.31 AS.seek 0.294ml @19:57:01.00 Exhaust.select :close @19:57:01.10 SSV.dial CBV @19:57:01.12 CBV.dial SSV @19:57:01.88 Allowing 185s for 25.0ml of remaining 225.0ml @19:57:01.89 Intake.select :open @19:57:04.02 Intake @34.0psia, vacuum from 20.0 to 24.0psi @19:57:04.04 SS.reconfigure forSampling @19:57:04.07 SS.seek 25.00ml @19:57:18.29 Stroke completed -- waiting for pressure to equalize ... @19:57:18.93 Intake.select :close @19:57:19.07 SSV.dial :exhaust @19:57:19.81 Exhaust.select :open @19:57:19.91 SS.reconfigure SSexhale @19:57:19.95 SS.seek 0.00ml @19:57:28.81 Exhaust.select :close @19:57:28.91 SSV.dial CBV @19:57:28.93 CBV.dial SSV @19:57:29.68 Allowing 185s for 25.0ml of remaining 200.0ml @19:57:29.70 Intake.select :open @19:57:31.83 Intake @34.1psia, vacuum from 20.0 to 24.0psi @19:57:31.85 SS.reconfigure forSampling @19:57:31.88 SS.seek 25.00ml @19:57:45.56 Stroke completed -- waiting for pressure to equalize ... @19:57:46.20 Intake.select :close @19:57:46.34 SSV.dial :exhaust @19:57:47.07 Exhaust.select :open @19:57:47.18 SS.reconfigure SSexhale @19:57:47.22 SS.seek 0.00ml @19:57:55.78 Exhaust.select :close @19:57:55.88 SSV.dial CBV @19:57:55.90 CBV.dial SSV @19:57:56.65 Allowing 185s for 25.0ml of remaining 175.0ml @19:57:56.67 Intake.select :open @19:57:58.88 Intake @34.1psia, vacuum from 20.0 to 24.0psi @19:57:58.90 SS.reconfigure forSampling @19:57:58.93 SS.seek 25.00ml @19:58:13.57 Stroke completed -- waiting for pressure to equalize ... @19:58:14.17 GoodNews.email "Then, in tube 2: wqPCR\n/home/esp/esp2/mission/14mar02sccwrp.rb:47",Subject => "Asleep until 18:28" @19:58:14.19 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @19:58:14.22 Retry #9 of 10 after 5:00 @19:58:14.26 Intake.select :close @19:58:14.41 SSV.dial :exhaust @19:58:15.15 Exhaust.select :open @19:58:15.25 SS.reconfigure SSexhale @19:58:15.29 SS.seek 0.00ml @19:58:23.93 Exhaust.select :close @19:58:24.03 SSV.dial CBV @19:58:24.05 CBV.dial SSV @19:58:24.81 Allowing 185s for 25.0ml of remaining 150.0ml @19:58:24.83 Intake.select :open @19:58:26.96 Intake @34.0psia, vacuum from 20.0 to 24.0psi @19:58:26.98 SS.reconfigure forSampling @19:58:27.01 SS.seek 25.00ml @19:58:40.81 Stroke completed -- waiting for pressure to equalize ... @19:58:41.45 Intake.select :close @19:58:41.59 SSV.dial :exhaust @19:58:42.34 Exhaust.select :open @19:58:42.44 SS.reconfigure SSexhale @19:58:42.48 SS.seek 0.00ml @19:58:51.04 Exhaust.select :close @19:58:51.14 SSV.dial CBV @19:58:51.16 CBV.dial SSV @19:58:51.92 Allowing 185s for 25.0ml of remaining 125.0ml @19:58:51.93 Intake.select :open @19:58:54.06 Intake @34.0psia, vacuum from 20.0 to 24.0psi @19:58:54.08 SS.reconfigure forSampling @19:58:54.11 SS.seek 25.00ml @19:59:08.32 Stroke completed -- waiting for pressure to equalize ... @19:59:08.96 Intake.select :close @19:59:09.11 SSV.dial :exhaust @19:59:09.84 Exhaust.select :open @19:59:09.95 SS.reconfigure SSexhale @19:59:09.99 SS.seek 0.00ml @19:59:18.70 Exhaust.select :close @19:59:18.80 SSV.dial CBV @19:59:18.82 CBV.dial SSV @19:59:19.58 Allowing 185s for 25.0ml of remaining 100.0ml @19:59:19.60 Intake.select :open @19:59:21.73 Intake @33.9psia, vacuum from 20.0 to 24.0psi @19:59:21.75 SS.reconfigure forSampling @19:59:21.78 SS.seek 25.00ml @19:59:36.64 Stroke completed -- waiting for pressure to equalize ... @19:59:37.28 Intake.select :close @19:59:37.42 SSV.dial :exhaust @19:59:38.16 Exhaust.select :open @19:59:38.26 SS.reconfigure SSexhale @19:59:38.30 SS.seek 0.00ml @19:59:43.74 delay 30 seconds @19:59:46.83 Exhaust.select :close @19:59:46.93 SSV.dial CBV @19:59:46.95 CBV.dial SSV @19:59:47.70 Allowing 185s for 25.0ml of remaining 75.0ml @19:59:47.72 Intake.select :open @19:59:49.85 Intake @34.0psia, vacuum from 20.0 to 24.0psi @19:59:49.87 SS.reconfigure forSampling @19:59:49.90 SS.seek 25.00ml @20:00:03.65 Stroke completed -- waiting for pressure to equalize ... @20:00:04.29 Intake.select :close @20:00:04.43 SSV.dial :exhaust @20:00:05.17 Exhaust.select :open @20:00:05.27 SS.reconfigure SSexhale @20:00:05.31 SS.seek 0.00ml @20:00:13.75 ATV.dial :waste,via => air3 @20:00:14.11 Exhaust.select :close @20:00:14.21 SSV.dial CBV @20:00:14.23 CBV.dial SSV @20:00:14.99 Allowing 185s for 25.0ml of remaining 50.0ml @20:00:15.00 Intake.select :open @20:00:15.19 AS.reconfigure ASconfig @20:00:15.22 AS.seek empty @20:00:17.13 Intake @34.1psia, vacuum from 20.0 to 24.0psi @20:00:17.15 SS.reconfigure forSampling @20:00:17.18 SS.seek 25.00ml @20:00:30.81 Stroke completed -- waiting for pressure to equalize ... @20:00:31.45 Intake.select :close @20:00:31.59 SSV.dial :exhaust @20:00:32.33 Exhaust.select :open @20:00:32.43 SS.reconfigure SSexhale @20:00:32.47 SS.seek 0.00ml @20:00:32.71 Parking Bleach in LysateSource line and SPEcolumn @20:00:32.72 ATV.dial :air3 @20:00:33.29 AS.reconfigure ASslow1 @20:00:33.32 AS.seek 0.029ml @20:00:39.76 ATV.dial :ElutionWater @20:00:40.26 AS.seek 0.059ml @20:00:41.06 Exhaust.select :close @20:00:41.16 SSV.dial CBV @20:00:41.18 CBV.dial SSV @20:00:41.94 Allowing 185s for 25.0ml of remaining 25.0ml @20:00:41.95 Intake.select :open @20:00:44.08 Intake @34.1psia, vacuum from 20.0 to 24.0psi @20:00:44.10 SS.reconfigure forSampling @20:00:44.13 SS.seek 24.98ml @20:00:46.68 ATV.dial :air3 @20:00:47.06 AS.seek 0.069ml @20:00:49.36 delay 2 seconds @20:00:51.37 ATV.dial :bleach @20:00:51.74 AS.seek 0.709ml @20:00:58.28 Stroke completed -- waiting for pressure to equalize ... @20:00:58.92 Intake.select :close @20:00:59.06 SSV.dial :exhaust @20:00:59.81 Exhaust.select :open @20:00:59.91 SS.reconfigure SSexhale @20:00:59.95 SS.seek 0.00ml @20:01:08.45 Exhaust.select :close @20:01:08.55 Sampled 1000.0ml @20:01:08.56 collectSample: Complete @20:01:08.57 Drying puck before fixing for 04:00.00 @20:01:08.58 CBV.dial :waste2b @20:01:09.33 delay 1:00 @20:02:09.33 CSV.dial :air @20:02:09.98 CS.reconfigure CSfast @20:02:10.01 CS.seek 10.000ml @20:02:33.45 CBV.dial between CSV and waste2b @20:02:33.76 Pumping wcr puck from tube 2 up to 55psi... @20:02:33.81 CBV.dial SSV @20:02:35.23 CBV.dial between CSV and waste2b @20:02:36.16 CSV.dial between air and CTV @20:02:36.46 CS.seek 7.177ml @20:02:43.65 CTV.dial CSV,avoiding => air @20:02:43.67 CSV.dial CTV @20:02:44.41 CS.reconfigure CSpressurize @20:02:44.44 CS.seek 0.000ml @20:03:02.50 delay 3 seconds @20:03:03.17 CSV.dial :air @20:03:03.68 CS.reconfigure CSfast @20:03:03.72 CS.seek 10.000ml @20:03:05.51 ATV.dial :air3 @20:03:05.88 delay 3 seconds @20:03:08.93 AS.seek 0.734ml @20:03:14.27 delay 3 seconds @20:03:14.29 GoodNews.email "Then, in tube 2: wqPCR\n/home/esp/esp2/mission/14mar02sccwrp.rb:47",Subject => "Asleep until 18:28" @20:03:14.31 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @20:03:14.32 Retry #10 of 10 after 5:00 @20:03:17.28 ATV.dial :bleach @20:03:17.77 AS.seek 0.719ml @20:03:21.11 delay 3 seconds @20:03:24.12 ATV.dial :AIV @20:03:25.31 AS.reconfigure ASconfig @20:03:25.34 AS.seek 0.015ml @20:03:27.78 CSV.dial between air and CTV @20:03:28.38 CS.seek 2.211ml @20:03:46.80 CTV.dial CSV,avoiding => air @20:03:46.81 CSV.dial CTV @20:03:47.19 CS.reconfigure CSpressurize @20:03:47.22 CS.seek 0.000ml @20:03:48.75 CBV.dial :waste2b @20:03:49.07 CS.reconfigure CSconfig @20:03:49.10 CS.seek empty @20:03:54.36 delay 1:00 @20:04:06.78 ATV.dial :waste @20:04:08.06 AS.seek empty @20:04:09.26 ATV.dial :air3 @20:04:09.83 AS.reconfigure ASslow1 @20:04:09.86 AS.seek 0.009ml @20:04:12.19 delay 2 seconds @20:04:14.20 ATV.dial :bleach @20:04:14.56 AS.seek 0.579ml @20:04:54.37 CTV.dial :air,avoiding => intake @20:04:55.64 Fix #1 of 2 (with rnal) for 02:00.00 @20:04:55.65 CS Pulling 2ml of rnal CSV.dial CSR @20:04:56.07 CSR.select :rnal @20:04:56.17 CS.seek 2.000ml @20:05:02.04 delay 5 seconds @20:05:07.05 CSR.select :air @20:05:07.14 CS.reconfigure CSfast @20:05:07.18 CS.seek 10.000ml @20:05:26.04 CBV.dial between CSV and waste2b @20:05:26.38 Pumping wcr puck from tube 2 up to 55psi... @20:05:26.43 CBV.dial SSV @20:05:27.84 CBV.dial between CSV and waste2b @20:05:28.78 CSV.dial between air and CTV @20:05:29.44 CS.seek 7.011ml @20:05:36.99 CTV.dial CSV,avoiding => air @20:05:37.01 CSV.dial CTV @20:05:38.30 CS.reconfigure CSpressurize @20:05:38.34 CS.seek 0.000ml @20:05:56.28 delay 2:00 @20:06:11.04 delay 3 seconds @20:06:14.05 ATV.dial :air3 @20:06:14.37 delay 3 seconds @20:06:17.43 AS.seek 0.604ml @20:06:22.78 delay 3 seconds @20:06:25.79 ATV.dial :bleach @20:06:26.17 AS.seek 0.589ml @20:06:29.48 delay 3 seconds @20:06:32.49 ATV.dial :ASV @20:06:33.37 ASV.dial :wasteR @20:06:33.86 AS.seek 0.000ml @20:07:56.29 CBV.dial :waste2b @20:07:56.62 CS.reconfigure CSconfig @20:07:56.65 CS.seek empty @20:07:57.47 delay 1:00 @20:08:14.33 GoodNews.email "Then, in tube 2: wqPCR\n/home/esp/esp2/mission/14mar02sccwrp.rb:47",Subject => "Asleep until 18:28" @20:08:14.35 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @20:08:14.36 BACfans.email "Can@20:08:13, 24.2C, 48% humidity, 14.6psia, 13.649V, 1.025A, 1.019A avg, 13.99W\nCTD data unavailable\nISUS data unavailable",Subject => "pcrBAC sampling at most 1000ml, wcr at most 1000ml" @20:08:14.38 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @20:08:14.39 Retry #1 of 10 after 5:00 @20:08:34.27 delay 1:00 @20:08:57.48 CTV.dial :air,avoiding => intake @20:08:58.74 Fix #2 of 2 (with rnal) for 02:00.00 @20:08:58.75 CS Pulling 2ml of rnal @20:08:58.76 CSV.dial CSR @20:08:59.18 CSR.select :rnal @20:08:59.28 CS.seek 2.000ml @20:09:05.15 delay 5 seconds @20:09:10.16 CSR.select :air @20:09:10.25 CS.reconfigure CSfast @20:09:10.29 CS.seek 10.000ml @20:09:29.15 CBV.dial between CSV and waste2b @20:09:29.47 Pumping wcr puck from tube 2 up to 55psi... @20:09:29.52 CBV.dial SSV @20:09:30.94 CBV.dial between CSV and waste2b @20:09:31.87 CSV.dial between air and CTV @20:09:32.52 CS.seek 6.813ml @20:09:34.28 AFV.select :carrierSolution @20:09:34.37 delay 2 seconds @20:09:36.38 AS.reconfigure ASconfig @20:09:36.41 AS.seek 1.000ml @20:09:40.60 CTV.dial CSV,avoiding => air @20:09:40.62 CSV.dial CTV @20:09:41.97 CS.reconfigure CSpressurize @20:09:42.00 CS.seek 0.000ml @20:09:59.30 delay 2:00 @20:10:35.02 delay 1 second @20:10:36.03 AFV.select :holdingCoil @20:10:36.12 delay 1 second @20:10:37.13 ATV.dial :ASV @20:10:37.14 ASV.dial :wasteR @20:10:37.15 AS.reconfigure ASslow1 @20:10:37.18 AS.seek 0.000ml @20:11:59.31 CBV.dial :waste2b @20:11:59.64 CS.reconfigure CSconfig @20:11:59.67 CS.seek empty @20:12:00.89 delay 1:00 @20:13:00.90 CTV.dial :air,avoiding => intake @20:13:02.21 Storing wcr puck in Collection Clamp to tube 1 @20:13:02.84 SC.reconfigure SCtight @20:13:02.87 SC.seek 1 @20:13:05.15 SC.reconfigure SCloose @20:13:05.34 Elbow.reconfigure TightElbow @20:13:05.38 Elbow.seek 1 @20:13:07.01 Forearm.reconfigure TightForearm @20:13:07.04 Forearm.seek 1 + 800 counts @20:13:08.12 SE.reconfigure SEpick @20:13:08.17 SE.seek beamBreak @20:13:11.12 SE.configure SEconfig @20:13:11.24 Elbow.reconfigure LimpElbow @20:13:11.28 Forearm.reconfigure LooseForearm @20:13:11.32 Forearm.seek 1 @20:13:11.53 Elbow.hold @20:13:11.59 Elbow.reconfigure TightElbow @20:13:11.66 SE.seek 1.04 Pucks below Top Plate @20:13:11.97 Forearm.coast @20:13:12.01 Forearm.seek 1 + 334 counts @20:13:12.30 Forearm.reconfigure TightForearm @20:13:12.34 Forearm.seek 1 + 800 counts @20:13:12.59 SE.seek 2.04 Pucks below Top Plate @20:13:13.15 Forearm.reconfigure LooseForearm @20:13:13.18 Forearm.seek retract @20:13:14.12 Elbow.seek CC @20:13:14.40 BACfans.email "Can@20:08:13, 24.2C, 48% humidity, 14.6psia, 13.649V, 1.025A, 1.019A avg, 13.99W\nCTD data unavailable\nISUS data unavailable",Subject => "pcrBAC sampling at most 1000ml, wcr at most 1000ml" @20:13:14.42 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @20:13:14.43 Retry #2 of 10 after 5:00 @20:13:15.69 Elbow.reconfigure LooseElbow @20:13:15.77 Forearm.seek CC @20:13:16.73 CC.seek sealed @20:13:17.67 Hand.grip @20:13:18.42 CC.seek open @20:13:21.81 Forearm.seek retract @20:13:22.76 Elbow.reconfigure TightElbow @20:13:22.79 Elbow.seek 1 @20:13:24.40 Elbow.reconfigure LimpElbow @20:13:24.44 Forearm.seek 1 @20:13:25.44 Elbow.hold @20:13:25.50 Elbow.reconfigure TightElbow @20:13:25.54 Hand.release @20:13:26.37 Forearm.coast @20:13:26.41 Forearm.seek 1 + 322 counts @20:13:26.65 Forearm.reconfigure TightForearm @20:13:26.69 Forearm.seek 1 + 800 counts @20:13:26.98 SE.seek down @20:13:28.07 Forearm.reconfigure LooseForearm @20:13:28.12 Forearm.seek retract @20:13:29.52 SE.coast @20:13:29.55 SC.coast @20:13:29.58 Starting finalPurge @20:13:29.59 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @20:13:29.74 Elbow.seek FlushPuck::Garage @20:13:31.52 Elbow.reconfigure LooseElbow @20:13:31.60 Forearm.seek garage @20:13:32.66 Hand.grip @20:13:33.51 Forearm.seek retract @20:13:34.53 Elbow.reconfigure TightElbow @20:13:35.17 Elbow.seek CC @20:13:37.91 Elbow.reconfigure LooseElbow @20:13:37.99 Forearm.reconfigure TightForearm @20:13:38.02 Forearm.seek CC @20:13:39.18 CC.seek closed @20:13:42.94 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 2.06 @20:13:43.01 Hand.release @20:13:43.83 Forearm.reconfigure LooseForearm @20:13:43.86 Forearm.seek retract @20:13:44.83 Flushing the Collection Valve::Manifold @20:13:44.93 CS Pulling 5ml of flush @20:13:44.94 CSV.dial CSR @20:13:45.44 CSR.select :flush @20:13:45.54 CS.seek 5.000ml @20:13:59.05 delay 5 seconds @20:14:01.46 delay 20 seconds @20:14:04.06
CSR.select :air @20:14:04.20 CS.reconfigure CSfast @20:14:04.23 CS.seek 7.500ml @20:14:10.83 CSV.dial :waste @20:14:12.10 CS.reconfigure CSconfig @20:14:12.13 CS.seek 0.000ml @20:14:21.47 ATV.dial :air3 @20:14:22.59 AS.seek 0.590ml @20:14:31.95
Flushing lower manifold's waste1 port @20:14:32.05 CSV.dial :flush @20:14:32.69 CS.reconfigure CSfast @20:14:32.72 CS.seek 5.000ml @20:14:44.89 delay 5 seconds @20:14:49.90 CSV.dial :air @20:14:50.48 CS.seek 7.000ml @20:14:55.82 CSV.dial CTV @20:14:55.84
CTV.dial CSV @20:14:57.15 CBV.dial :waste1 @20:14:57.90 CS.seek 0.000ml @20:15:14.29 Flushing lower manifold's waste2b port @20:15:14.39 CSV.dial :flush @20:15:15.18 CS.seek 5.000ml @20:15:27.32 delay 5 seconds @20:15:32.33 CSV.dial :air @20:15:32.88 CS.seek 7.000ml @20:15:38.32 CSV.dial CTV @20:15:38.34
CTV.dial CSV @20:15:38.78 CBV.dial :waste2b @20:15:39.52 CS.seek 0.000ml @20:15:55.98 Flushing upper manifold @20:15:56.08 CSV.dial :flush @20:15:56.88 CS.seek 5.000ml @20:16:09.01 delay 5 seconds @20:16:14.02 CSV.dial :air @20:16:14.57 CS.seek 7.000ml @20:16:19.95 CSV.dial CBV @20:16:19.97
CBV.dial CSV @20:16:21.08 CTV.dial :air @20:16:22.35 CS.seek 0.000ml @20:16:23.20 delay 3 seconds @20:16:26.21 ATV.dial :ASV @20:16:27.26 ASV.dial :wasteR @20:16:27.32 AS.seek 0.090ml @20:16:38.77
delay 7 seconds @20:16:45.78 CS.seek 3.000ml @20:16:53.36 CSV.dial :waste @20:16:53.87 CS.seek 0.000ml @20:17:01.54 CSV.dial :flush @20:17:01.98 CS.seek 3.000ml @20:17:09.61 delay 5 seconds @20:17:14.62 CSV.dial :air @20:17:15.50 CS.seek 5.000ml @20:17:20.86 CSV.dial :air @20:17:20.88 CS.seek 0.000ml @20:17:33.01 delay 7 seconds @20:17:40.02 CS.seek 3.000ml @20:17:47.70 CSV.dial :waste @20:17:48.49 CS.seek 0.000ml @20:17:56.04 Disinfecting external Intake with mfbkill @20:17:56.72 CSV.dial CSR @20:17:57.98 CSR.select :mfbkill @20:17:58.09 CS.seek 8.000ml @20:18:09.53 delay 30 seconds @20:18:14.44 BACfans.email "Can@20:08:13, 24.2C, 48% humidity, 14.6psia, 13.649V, 1.025A, 1.019A avg, 13.99W\nCTD data unavailable\nISUS data unavailable",Subject => "pcrBAC sampling at most 1000ml, wcr at most 1000ml" @20:18:14.46 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @20:18:14.47 Retry #3 of 10 after 5:00 @20:18:17.01
delay 5 seconds @20:18:22.02 CSR.select :air @20:18:22.12 CS.seek 10.000ml @20:18:27.48 CSV.dial CBV @20:18:27.50
CBV.dial CSV @20:18:28.58 CTV.dial :intake @20:18:29.28 Intake.select :open @20:18:29.38 CS.seek 2.000ml @20:18:39.54 ATV.dial :extSrc @20:18:40.13 delay 3 seconds @20:18:43.14 ATV.dial :ElutionWater @20:18:44.06 AS.seek 0.120ml @20:18:48.60
Intake.select :close @20:18:48.69 CTV.dial :air @20:18:49.44 CS.reconfigure CSconfig @20:18:49.47 CS.seek 0.000ml @20:18:50.45 ATV.dial :air3 @20:18:50.85 AS.seek 0.130ml @20:18:53.15 delay 2 seconds @20:18:55.16 ATV.dial :bleach @20:18:55.55 AS.seek 0.230ml @20:18:55.59
Flushing lower manifold's waste1 port @20:18:55.60 CS Pulling 4ml of mfbkill @20:18:55.61 CSV.dial CSR @20:18:56.65 CSR.select :mfbkill @20:18:56.75 CS.seek 4.000ml @20:19:07.70 delay 5 seconds @20:19:12.71 CSR.select :air @20:19:12.80 CS.reconfigure CSfast @20:19:12.84 CS.seek 10.000ml @20:19:16.23 delay 3 seconds @20:19:19.24 ATV.dial :air3 @20:19:19.66 delay 3 seconds @20:19:22.72 AS.seek 0.255ml @20:19:27.14 CSV.dial CTV @20:19:27.16
CTV.dial CSV @20:19:28.09 delay 3 seconds @20:19:28.49
CBV.dial :waste1 @20:19:28.94 CS.seek 0.000ml @20:19:31.11 ATV.dial :bleach @20:19:31.50 AS.seek 0.240ml @20:19:34.84 delay 3 seconds @20:19:37.85 ATV.dial :ASV @20:19:38.73 ASV.dial :SPEdelivery @20:19:39.21 AFV.select :carrierSolution @20:19:39.31 delay 2 seconds @20:19:41.32 AS.reconfigure ASconfig @20:19:41.35 AS.seek 0.500ml @20:19:52.10
Flushing lower manifold's waste2b port @20:19:52.11 CS Pulling 4ml of mfbkill @20:19:52.12 CSV.dial CSR @20:19:52.62 CSR.select :mfbkill @20:19:52.72 CS.reconfigure CSconfig @20:19:52.76 CS.seek 4.000ml @20:19:56.77 delay 1 second @20:19:57.78 AFV.select :holdingCoil @20:19:57.92 AS.reconfigure ASslow1 @20:19:57.95 AS.seek 0.170ml @20:20:03.73
delay 5 seconds @20:20:08.74 CSR.select :air @20:20:08.83 CS.reconfigure CSfast @20:20:08.87 CS.seek 10.000ml @20:20:23.20 CSV.dial CTV @20:20:23.22
CTV.dial CSV @20:20:23.73 CBV.dial :waste2b @20:20:24.45 CS.seek 0.000ml @20:20:47.54 Killing upper manifold @20:20:47.55 CS Pulling 4ml of mfbkill @20:20:47.56 CSV.dial CSR @20:20:48.07 CSR.select :mfbkill @20:20:48.17 CS.reconfigure CSconfig @20:20:48.21 CS.seek 4.000ml @20:20:59.10 delay 5 seconds @20:21:04.11 CSR.select :air @20:21:04.21 CS.reconfigure CSfast @20:21:04.25 CS.seek 10.000ml @20:21:05.54 SPE.hold 2:46:40,95.0C @20:21:05.56 delay 3:00 @20:21:18.97 CSV.dial CBV @20:21:18.99
CBV.dial CSV @20:21:20.05 CTV.dial :air @20:21:21.36 CS.seek 0.000ml @20:21:45.25 delay 7 seconds @20:21:52.26 CS.seek 3.000ml @20:22:00.19 CSV.dial :waste @20:22:00.64 CS.seek 0.000ml @20:22:08.44 Flushing upper manifold @20:22:08.54 CSV.dial :flush @20:22:08.98 CS.seek 5.000ml @20:22:21.50 delay 5 seconds @20:22:26.51 CSV.dial :air @20:22:27.01 CS.seek 7.000ml @20:22:32.50 CSV.dial CBV @20:22:32.52
CBV.dial CSV @20:22:33.63 CTV.dial :air @20:22:33.64 CS.seek 0.000ml @20:22:50.69 delay 7 seconds @20:22:57.70 CS.seek 3.000ml @20:23:05.53 CSV.dial :waste @20:23:06.05 CS.seek 0.000ml @20:23:14.43 CSV.dial :flush @20:23:14.47 BACfans.email "Can@20:08:13, 24.2C, 48% humidity, 14.6psia, 13.649V, 1.025A, 1.019A avg, 13.99W\nCTD data unavailable\nISUS data unavailable",Subject => "pcrBAC sampling at most 1000ml, wcr at most 1000ml" @20:23:14.49 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @20:23:14.50 Retry #4 of 10 after 5:00 @20:23:14.88
CS.seek 3.000ml @20:23:22.72 delay 5 seconds @20:23:27.73 CSV.dial :air @20:23:28.22 CS.seek 5.000ml @20:23:33.78 CSV.dial :air @20:23:33.80 CS.seek 0.000ml @20:23:46.18 delay 7 seconds @20:23:53.19 CS.seek 3.000ml @20:24:01.00 CSV.dial :waste @20:24:01.82 CS.seek 0.000ml @20:24:05.57 AFV.select :carrierSolution @20:24:05.66 delay 2 seconds @20:24:07.67 AS.reconfigure ASconfig @20:24:07.70 AS.seek 1.000ml @20:24:09.57
Flushing lower manifold's waste1 port @20:24:09.66 CSV.dial :flush @20:24:10.19 CS.seek 5.000ml @20:24:22.68 delay 5 seconds @20:24:27.69 CSV.dial :air @20:24:28.18 CS.seek 7.000ml @20:24:33.65 CSV.dial CTV @20:24:33.67
CTV.dial CSV @20:24:35.01 CBV.dial :waste1 @20:24:35.47 CS.seek 0.000ml @20:24:52.24 Flushing lower manifold's waste2b port @20:24:52.34 CSV.dial :flush @20:24:53.14 CS.seek 5.000ml @20:24:56.39 delay 1 second @20:24:57.40 AFV.select :holdingCoil @20:24:57.49 delay 1 second @20:24:58.50 ASV.dial :wasteD @20:24:59.51 AS.seek 0.000ml @20:25:05.34
delay 5 seconds @20:25:10.35 CSV.dial :air @20:25:10.92 CS.seek 7.000ml @20:25:16.49 CSV.dial CTV @20:25:16.51
CTV.dial CSV @20:25:16.95 CBV.dial :waste2b @20:25:17.70 CS.seek 0.000ml @20:25:34.37 Disinfecting external Intake with kill @20:25:34.47 CSV.dial CSR @20:25:34.91 CSR.select :kill @20:25:35.01 CS.seek 8.000ml @20:25:54.15 delay 5 seconds @20:25:58.13 Priming Eluate @20:25:58.14 AFV.select :carrierSolution @20:25:58.24 delay 2 seconds @20:25:59.16
CSR.select :air @20:25:59.25 CS.seek 10.000ml @20:26:00.25 AS.seek 0.500ml @20:26:04.65 CSV.dial CBV @20:26:04.67
CBV.dial CSV @20:26:05.72 CTV.dial :intake @20:26:06.44 Intake.select :open @20:26:06.54 CS.seek 2.000ml @20:26:26.18 Intake.select :close @20:26:26.27 CTV.dial :air @20:26:27.00 CS.reconfigure CSconfig @20:26:27.03 CS.seek 0.000ml @20:26:29.70 delay 1 second @20:26:30.71 AFV.select :holdingCoil @20:26:30.80 ATV.dial ASV @20:26:30.81 ASV.dial :SPErecovery @20:26:31.45 Swish #1 of 2 @20:26:31.51 AS.reconfigure ASslow0 @20:26:31.54 AS.seek 0.600ml @20:26:32.93
CSV.dial CSR @20:26:34.00 CSR.select :flush @20:26:34.10 CS.seek 5.000ml @20:26:42.04 AS.seek 0.500ml @20:26:47.58
delay 5 seconds @20:26:52.54 Swish #2 of 2 @20:26:52.60 AS.seek 0.600ml @20:26:52.62
CSR.select :air @20:26:52.73 CS.seek 10.000ml @20:27:03.12 AS.seek 0.500ml @20:27:06.17
CSV.dial CBV @20:27:07.25 CS.seek 0.000ml @20:27:13.59 delay 3 seconds @20:27:16.65 AS.reconfigure ASslow1 @20:27:16.68 AS.seek 0.676ml @20:27:33.52
Garaging FlushPuck in Collection Clamp @20:27:33.58 Elbow.reconfigure TightElbow @20:27:33.61 Elbow.seek CC @20:27:33.71 Elbow.reconfigure LooseElbow @20:27:33.79 Forearm.seek CC @20:27:34.73 CC.seek sealed @20:27:35.70 Hand.grip @20:27:36.39 CC.seek open @20:27:40.20 Forearm.seek retract @20:27:41.17 Elbow.reconfigure TightElbow @20:27:41.20 Elbow.seek garage @20:27:43.97 Elbow.reconfigure LooseElbow @20:27:44.05 Forearm.reconfigure TightForearm @20:27:44.08 Forearm.seek garage @20:27:45.25 Hand.release @20:27:46.05 Forearm.reconfigure LooseForearm @20:27:46.09 Forearm.seek retract @20:27:52.88 delay 3 seconds @20:27:55.89 Eluate primed @20:27:55.90 ATV.dial :waste2 @20:27:56.42 AS.reconfigure ASnorm @20:27:56.45 AS.seek 0.000ml @20:28:14.50 BACfans.email "Can@20:08:13, 24.2C, 48% humidity, 14.6psia, 13.649V, 1.025A, 1.019A avg, 13.99W\nCTD data unavailable\nISUS data unavailable",Subject => "pcrBAC sampling at most 1000ml, wcr at most 1000ml" @20:28:14.52 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @20:28:14.53 Retry #5 of 10 after 5:00 @20:28:31.33 SPE.coast @20:28:31.41 ASV.dial :SPErecovery @20:28:31.43 Setting up PCR for IPCem -- writing /var/log/esp/IPCem14mar0220h1000ml.pcr @20:28:31.44 Building PCR Slug from ASV with MM3 mix, PP5 primer @20:28:31.45 Priming with carrier ATV.dial ARV @20:28:32.25 ARV.dial :carrier @20:28:32.54 AS.seek 0.500ml @20:28:58.46 AFV.select :carrierSolution @20:28:58.56 delay 2 seconds @20:29:00.57 AS.reconfigure ASconfig @20:29:00.61 AS.seek 1.000ml @20:29:30.03 delay 1 second @20:29:31.04 AFV.select :holdingCoil @20:29:31.13 delay 1 second @20:29:32.14 ATV.dial :waste @20:29:33.40 AS.reconfigure ASnorm @20:29:33.43 AS.seek empty @20:30:24.92 AFV.select :carrierSolution @20:30:25.02 delay 2 seconds @20:30:27.03 AS.reconfigure ASconfig @20:30:27.06 AS.seek 0.500ml @20:30:56.55 delay 1 second @20:30:57.56 AFV.select :holdingCoil @20:30:57.65 Building Slug @20:30:57.66 ATV.dial :ARV @20:30:59.00 delay 2 seconds @20:31:01.01 ARV.dial :air1 @20:31:01.34 delay 2 seconds @20:31:03.40 AS.reconfigure ASslow2 @20:31:03.43 AS.seek 0.506ml @20:31:09.83 delay 2 seconds @20:31:11.84 ARV.dial :MM3 @20:31:12.70 delay 2 seconds @20:31:14.71 Adding MM3 @20:31:14.77 AS.seek 0.524ml @20:31:33.58 delay 25 seconds @20:31:58.59 ARV.dial :PP5 @20:31:58.91 Adding PP5 @20:31:58.92 delay 2 seconds @20:32:00.97 AS.seek 0.530ml @20:32:07.56 delay 10 seconds @20:32:17.57 ARV.dial :air3 @20:32:17.90 adding ARV air @20:32:17.91 delay 2 seconds @20:32:19.96 AS.seek 0.536ml @20:32:26.39 delay 5 seconds @20:32:31.40 ARV.dial :carrier @20:32:32.51 delay 7 seconds @20:32:39.57 AS.seek 0.594ml @20:33:14.53 BACfans.email "Can@20:08:13, 24.2C, 48% humidity, 14.6psia, 13.649V, 1.025A, 1.019A avg, 13.99W\nCTD data unavailable\nISUS data unavailable",Subject => "pcrBAC sampling at most 1000ml, wcr at most 1000ml" @20:33:14.55 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @20:33:14.56 Retry #6 of 10 after 5:00 @20:33:38.85 delay 5 seconds @20:33:43.86 ATV.dial :ASV @20:33:44.19 delay 2 seconds @20:33:46.25 AS.seek 0.600ml @20:33:52.72 delay 4 seconds @20:33:56.73 ATV.dial ARV @20:33:57.05 delay 2 seconds @20:33:59.06 ARV.dial :carrier @20:33:59.07 delay 2 seconds @20:34:01.12 AS.seek 0.650ml @20:34:52.33 delay 4 seconds @20:34:56.34 ATV.dial PCR @20:34:56.84 ASV.dial :waste1 @20:34:57.52 ARV.dial :air1 @20:34:57.96 Positioning Slug by 0.324ml @20:34:58.01 AS.seek 0.326ml @20:38:14.58 BACfans.email "Can@20:08:13, 24.2C, 48% humidity, 14.6psia, 13.649V, 1.025A, 1.019A avg, 13.99W\nCTD data unavailable\nISUS data unavailable",Subject => "pcrBAC sampling at most 1000ml, wcr at most 1000ml" @20:38:14.60 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @20:38:14.61 Retry #7 of 10 after 5:00 @20:40:29.04 delay 5 seconds @20:40:34.05 ATV.dial ARV @20:40:34.57 ASV.dial :PCRlock @20:40:34.91 delay 5 seconds @20:40:39.92 Running IPCem ProfileEM59C thermocycling @20:40:39.93 PCR.program ProfileEM59C @20:40:40.04 ProfileEM59C should complete in < 2:11:38 @20:40:40.05 PCR.run 2:49:32.5 @20:43:14.62 BACfans.email "Can@20:08:13, 24.2C, 48% humidity, 14.6psia, 13.649V, 1.025A, 1.019A avg, 13.99W\nCTD data unavailable\nISUS data unavailable",Subject => "pcrBAC sampling at most 1000ml, wcr at most 1000ml" @20:43:14.64 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @20:43:14.65 Retry #8 of 10 after 5:00 @20:48:14.65 BACfans.email "Can@20:08:13, 24.2C, 48% humidity, 14.6psia, 13.649V, 1.025A, 1.019A avg, 13.99W\nCTD data unavailable\nISUS data unavailable",Subject => "pcrBAC sampling at most 1000ml, wcr at most 1000ml" @20:48:14.67 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @20:48:14.68 Retry #9 of 10 after 5:00 @20:53:14.70 BACfans.email "Can@20:08:13, 24.2C, 48% humidity, 14.6psia, 13.649V, 1.025A, 1.019A avg, 13.99W\nCTD data unavailable\nISUS data unavailable",Subject => "pcrBAC sampling at most 1000ml, wcr at most 1000ml" @20:53:14.72 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @20:53:14.73 Retry #10 of 10 after 5:00 @20:58:14.73 BACfans.email "Can@20:08:13, 24.2C, 48% humidity, 14.6psia, 13.649V, 1.025A, 1.019A avg, 13.99W\nCTD data unavailable\nISUS data unavailable",Subject => "pcrBAC sampling at most 1000ml, wcr at most 1000ml" @20:58:14.75 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @22:41:03.66 PCR.off @22:41:04.18 PCR for IPCem completed -- closed /var/log/esp/IPCem14mar0220h1000ml.pcr @22:41:04.23 Cleaning PCR @22:41:04.24 AFV.select :carrierSolution @22:41:04.33 delay 2 seconds @22:41:06.34 AS.reconfigure ASconfig @22:41:06.37 AS.seek 0.500ml @22:41:16.82 delay 1 second @22:41:17.83 AFV.select :holdingCoil @22:41:17.92 ASV.dial :waste1 @22:41:18.66 ATV.dial :air3 @22:41:19.59 AS.reconfigure ASslow1 @22:41:19.63 AS.seek 0.510ml @22:41:21.93 delay 2 seconds @22:41:23.94 ATV.dial :bleach @22:41:24.43 AS.seek 0.810ml @22:42:25.86 delay 3 seconds @22:42:28.87 ATV.dial :air3 @22:42:29.27 delay 3 seconds @22:42:32.33 AS.seek 0.835ml @22:42:37.64 delay 3 seconds @22:42:40.65 ATV.dial :bleach @22:42:41.13 AS.seek 0.820ml @22:42:44.44 delay 3 seconds @22:42:47.45 ARV.dial :waste @22:42:47.93 delay 3 seconds @22:42:50.94 ATV.dial :ARV @22:42:51.68 AS.reconfigure ASconfig @22:42:51.72 AS.seek 0.470ml @22:43:12.41 AFV.select :carrierSolution @22:43:12.50 delay 2 seconds @22:43:14.51 AS.seek 1.000ml @22:43:45.72 delay 1 second @22:43:46.73 AFV.select :holdingCoil @22:43:46.82 delay 1 second @22:43:47.83 AS.reconfigure ASfast @22:43:47.86 AS.seek 0.000ml @22:44:15.56 AFV.select :carrierSolution @22:44:15.65 delay 2 seconds @22:44:17.66 AS.reconfigure ASconfig @22:44:17.69 AS.seek 1.000ml @22:45:16.23 delay 1 second @22:45:17.24 AFV.select :holdingCoil @22:45:17.33 delay 1 second @22:45:18.34 AS.reconfigure ASfast @22:45:18.37 AS.seek 0.000ml @22:45:46.11 AFV.select :carrierSolution @22:45:46.20 delay 2 seconds @22:45:48.21 AS.reconfigure ASconfig @22:45:48.24 AS.seek 1.000ml @22:46:46.85 delay 1 second @22:46:47.86 AFV.select :holdingCoil @22:46:47.95 delay 1 second @22:46:48.96 AS.reconfigure ASfast @22:46:48.99 AS.seek 0.000ml @22:47:16.71 ARV.dial between carrier and air1 @22:47:17.67 ATV.dial :air3 @22:47:18.70 AS.reconfigure ASslow1 @22:47:18.73 AS.seek 0.010ml @22:47:21.04 delay 2 seconds @22:47:23.05 ATV.dial :bleach @22:47:23.42 AS.seek 0.810ml @22:50:06.83 delay 3 seconds @22:50:09.84 ATV.dial :air3 @22:50:10.17 delay 3 seconds @22:50:13.23 AS.seek 0.835ml @22:50:18.54 delay 3 seconds @22:50:21.55 ATV.dial :bleach @22:50:21.92 AS.seek 0.820ml @22:50:25.23 delay 3 seconds @22:50:28.24 ATV.dial PCR @22:50:28.57 AS.reconfigure ASconfig @22:50:28.60 AS.seek empty @22:51:16.71 PCR.program :bleachProfile @22:51:16.76 PCR program should complete in < 2:45 @22:51:16.77 PCR.run 10:00 @22:54:11.94 PCR.off @22:54:11.97 AFV.select :carrierSolution @22:54:12.06 delay 2 seconds @22:54:14.07 AS.seek 1.000ml @22:55:12.74 delay 1 second @22:55:13.75 AFV.select :holdingCoil @22:55:13.84 delay 1 second @22:55:14.85 AS.reconfigure ASfast @22:55:14.88 AS.seek 0.000ml @22:55:42.58 delay 3 seconds @22:55:45.59 AFV.select :carrierSolution @22:55:45.68 delay 2 seconds @22:55:47.69 AS.reconfigure ASconfig @22:55:47.72 AS.seek 1.000ml @22:56:46.30 delay 1 second @22:56:47.31 AFV.select :holdingCoil @22:56:47.40 delay 1 second @22:56:48.41 AS.reconfigure ASfast @22:56:48.54 AS.seek 0.000ml @22:57:16.28 delay 3 seconds @22:57:19.29 AFV.select :carrierSolution @22:57:19.38 delay 2 seconds @22:57:21.39 AS.reconfigure ASconfig @22:57:21.42 AS.seek 1.000ml @22:58:20.00 delay 1 second @22:58:21.01 AFV.select :holdingCoil @22:58:21.10 delay 1 second @22:58:22.11 AS.reconfigure ASfast @22:58:22.14 AS.seek 0.000ml @22:58:49.87 delay 3 seconds @22:58:52.88 AFV.select :carrierSolution @22:58:52.97 delay 2 seconds @22:58:54.98 AS.reconfigure ASconfig @22:58:55.01 AS.seek 1.000ml @22:59:53.63 delay 1 second @22:59:54.64 AFV.select :holdingCoil @22:59:54.73 delay 1 second @22:59:55.74 AS.reconfigure ASfast @22:59:55.77 AS.seek 0.000ml @23:00:23.51 delay 3 seconds @23:00:26.52 AFV.select :carrierSolution @23:00:26.61 delay 2 seconds @23:00:28.62 AS.reconfigure ASconfig @23:00:28.65 AS.seek 1.000ml @23:01:27.27 delay 1 second @23:01:28.28 AFV.select :holdingCoil @23:01:28.37 delay 1 second @23:01:29.38 AS.reconfigure ASnorm @23:01:29.41 AS.seek 0.000ml @23:02:20.88 Setting up PCR for IPCap -- writing /var/log/esp/IPCap14mar0223h1000ml.pcr @23:02:20.89 Building PCR Slug from ASV with MM1 mix, PP2 primer @23:02:20.90 Priming with carrier @23:02:20.91 ATV.dial ARV @23:02:21.41 ARV.dial :carrier @23:02:21.69 AS.seek 0.500ml @23:02:47.63 AFV.select :carrierSolution @23:02:47.73 delay 2 seconds @23:02:49.74 AS.reconfigure ASconfig @23:02:49.78 AS.seek 1.000ml @23:03:19.19 delay 1 second @23:03:20.20 AFV.select :holdingCoil @23:03:20.29 delay 1 second @23:03:21.30 ATV.dial :waste @23:03:22.54 AS.reconfigure ASnorm @23:03:22.57 AS.seek empty @23:04:14.05 AFV.select :carrierSolution @23:04:14.15 delay 2 seconds @23:04:16.16 AS.reconfigure ASconfig @23:04:16.19 AS.seek 0.500ml @23:04:45.65 delay 1 second @23:04:46.66 AFV.select :holdingCoil @23:04:46.75 Building Slug @23:04:46.76 ATV.dial :ARV @23:04:48.00 delay 2 seconds @23:04:50.01 ARV.dial :air1 @23:04:50.34 delay 2 seconds @23:04:52.40 AS.reconfigure ASslow2 @23:04:52.43 AS.seek 0.506ml @23:04:58.70 delay 2 seconds @23:05:00.71 ARV.dial :MM1 @23:05:01.28 delay 2 seconds @23:05:03.29 Adding MM1 @23:05:03.35 AS.seek 0.524ml @23:05:22.08 delay 25 seconds @23:05:47.09 ARV.dial :PP2 @23:05:47.43 Adding PP2 @23:05:47.44 delay 2 seconds @23:05:49.49 AS.seek 0.530ml @23:05:56.11 delay 10 seconds @23:06:06.12 ARV.dial :air2 @23:06:06.44 adding ARV air @23:06:06.45 delay 2 seconds @23:06:08.50 AS.seek 0.536ml @23:06:15.17 delay 5 seconds @23:06:20.18 ARV.dial :carrier @23:06:21.21 delay 7 seconds @23:06:28.27 AS.seek 0.594ml @23:07:27.67 delay 5 seconds @23:07:32.68 ATV.dial :ASV @23:07:33.08 delay 2 seconds @23:07:35.14 AS.seek 0.600ml @23:07:41.50 delay 4 seconds @23:07:46.02 ATV.dial ARV @23:07:46.35 delay 2 seconds @23:07:48.36 ARV.dial :carrier @23:07:48.37 delay 2 seconds @23:07:50.42 AS.seek 0.650ml @23:08:41.66 delay 4 seconds @23:08:45.67 ATV.dial PCR @23:08:46.17 ASV.dial :waste1 @23:08:46.18 ARV.dial :air1 @23:08:46.53 Positioning Slug by 0.324ml @23:08:46.59 AS.seek 0.326ml @23:14:17.39 delay 5 seconds @23:14:22.40 ATV.dial ARV @23:14:22.91 ASV.dial :PCRlock @23:14:23.35 delay 5 seconds @23:14:28.36 Running IPCap Profile59C thermocycling @23:14:28.37 PCR.program Profile59C @23:14:28.47 Profile59C should complete in < 1:39:08 @23:14:28.48 PCR.run 2:08:55 @00:41:45.19PST03-Mar-14 PCR.off @00:41:45.72 PCR for IPCap completed -- closed /var/log/esp/IPCap14mar0223h1000ml.pcr @00:41:45.73 Full MFB Decontamination @00:41:45.74 Decon extraction column and decon AIV @00:41:45.75 SPE.hold 2:46:40,55.0C @00:41:45.77 ASV.dial :SPEdelivery @00:41:46.42 delay 20 seconds @00:42:06.43 AFV.select :carrierSolution @00:42:06.52 delay 2 seconds @00:42:08.53 AS.reconfigure ASconfig @00:42:08.56 AS.seek 0.500ml @00:42:18.99 delay 1 second @00:42:20.00 AFV.select :holdingCoil @00:42:20.09 ATV.dial :air1 @00:42:21.68 AS.reconfigure ASslow1 @00:42:21.71 AS.seek 0.520ml @00:42:26.06 ATV.dial :ASV @00:42:27.31 ASV.dial :SPErecovery @00:42:28.40 AS.seek 0.620ml @00:42:49.04 ATV.dial :waste @00:42:50.50 AS.reconfigure ASnorm @00:42:50.53 AS.seek empty @00:43:22.60 AFV.select :holdingCoil @00:43:22.61 ATV.dial :waste @00:43:22.62 AS.reconfigure ASconfig @00:43:22.66 AS.seek 0.000ml @00:43:22.96 AFV.select :carrierSolution @00:43:23.06 delay 2 seconds @00:43:25.07 AS.seek 0.500ml @00:43:54.49 delay 1 second @00:43:55.50 AFV.select :holdingCoil @00:43:55.59 ATV.dial :air3 @00:43:56.13 AS.reconfigure ASslow1 @00:43:56.16 AS.seek 0.520ml @00:44:00.51 ATV.dial :air3 @00:44:00.57 AS.seek 0.530ml @00:44:02.86 delay 2 seconds @00:44:04.87 ATV.dial :bleach @00:44:05.24 AS.seek 0.710ml @00:44:42.21 delay 3 seconds @00:44:45.22 ATV.dial :air3 @00:44:45.55 delay 3 seconds @00:44:48.61 AS.seek 0.735ml @00:44:53.97 delay 3 seconds @00:44:56.98 ATV.dial :bleach @00:44:57.35 AS.seek 0.720ml @00:45:00.61 delay 3 seconds @00:45:03.62 ATV.dial ASV @00:45:04.49 ASV.dial :SPErecovery @00:45:04.55 AS.seek 0.365ml @00:46:17.19 ATV.dial :waste @00:46:18.64 AS.reconfigure ASnorm @00:46:18.67 AS.seek empty @00:46:37.70 ATV.dial :air3 @00:46:38.35 AS.reconfigure ASslow1 @00:46:38.38 AS.seek 0.009ml @00:46:40.73 delay 2 seconds @00:46:42.74 ATV.dial :bleach @00:46:43.11 AS.seek 0.579ml @00:48:39.59 delay 3 seconds @00:48:42.60 ATV.dial :air3 @00:48:43.03 delay 3 seconds @00:48:46.09 AS.seek 0.604ml @00:48:51.45 delay 3 seconds @00:48:54.46 ATV.dial :bleach @00:48:54.95 AS.seek 0.589ml @00:48:58.24 delay 3 seconds @00:49:01.25 ATV.dial :ASV @00:49:02.14 ASV.dial :wasteR @00:49:02.91 AS.seek 0.000ml @00:51:03.34 delay 1:00 @00:52:03.35 ATV.dial ASV @00:52:03.36 AFV.select :carrierSolution @00:52:03.45 delay 2 seconds @00:52:05.46 AS.reconfigure ASconfig @00:52:05.49 AS.seek 1.000ml @00:53:04.04 delay 1 second @00:53:05.05 AFV.select :holdingCoil @00:53:05.14 delay 1 second @00:53:06.15 ASV.dial :wasteR @00:53:06.16 AS.reconfigure ASslow1 @00:53:06.19 AS.seek 0.000ml @00:56:30.44 delay 10 seconds @00:56:40.45 ATV.dial :air3 @00:56:41.55 AS.seek 0.010ml @00:56:43.86 delay 2 seconds @00:56:45.87 ATV.dial :bleach @00:56:46.24 AS.seek 0.580ml @00:58:42.75 delay 3 seconds @00:58:45.76 ATV.dial :air3 @00:58:46.19 delay 3 seconds @00:58:49.25 AS.seek 0.605ml @00:58:54.61 delay 3 seconds @00:58:57.62 ATV.dial :bleach @00:58:58.00 AS.seek 0.590ml @00:59:01.31 delay 3 seconds @00:59:04.32 ATV.dial ASV @00:59:05.28 ASV.dial :SPEdelivery @00:59:05.76 AS.seek 0.000ml @01:01:06.34 delay 1:00 @01:02:06.35 AFV.select :carrierSolution @01:02:06.44 delay 2 seconds @01:02:08.45 AS.reconfigure ASconfig @01:02:08.49 AS.seek 1.000ml @01:03:07.12 delay 1 second @01:03:08.13 AFV.select :holdingCoil @01:03:08.22 delay 1 second @01:03:09.23 ATV.dial ASV @01:03:09.24 ASV.dial :SPEdelivery @01:03:09.25 AS.reconfigure ASslow1 @01:03:09.28 AS.seek 0.000ml @01:06:33.54 delay 10 seconds @01:06:43.55 ATV.dial :air2 @01:06:44.38 AS.reconfigure ASslow0 @01:06:44.41 AS.seek 0.800ml @01:08:06.37 delay 3 seconds @01:08:09.38 ATV.dial :ASV @01:08:10.18 ASV.dial :SPEdelivery @01:08:10.20 AS.reconfigure ASslow1 @01:08:10.23 AS.seek 0.030ml @01:10:47.52 delay 20 seconds @01:11:07.53 ATV.dial :waste,via => air3 @01:11:09.06 AS.reconfigure ASconfig @01:11:09.09 AS.seek empty @01:11:11.16 SPE.coast @01:11:11.24 Removing Bleach from Mixing Coil AFV.select :carrierSolution @01:11:11.33 delay 2 seconds @01:11:13.34 AS.seek 0.500ml @01:11:42.83 delay 1 second @01:11:43.84 AFV.select :holdingCoil @01:11:43.93 ATV.dial :ASV @01:11:45.37 ASV.dial :SPErecovery @01:11:46.05 AS.reconfigure ASslow1 @01:11:46.09 AS.seek 0.730ml @01:12:33.18 ASV.dial :wasteD @01:12:33.69 AS.reconfigure ASnorm @01:12:33.72 AS.seek 0.000ml @01:13:11.32 AFV.select :carrierSolution @01:13:11.41 delay 2 seconds @01:13:13.42 AS.reconfigure ASconfig @01:13:13.98 AS.seek 0.500ml @01:13:43.42 delay 1 second @01:13:44.43 AFV.select :holdingCoil @01:13:44.52 ATV.dial ASV @01:13:44.53 ASV.dial :SPErecovery @01:13:45.50 AS.reconfigure ASslow0 @01:13:45.53 AS.seek 0.300ml @01:14:06.23 ATV.dial :air2 @01:14:06.98 AS.reconfigure ASslow1 @01:14:07.01 AS.seek 0.320ml @01:14:11.38 ATV.dial ASV @01:14:12.14 AS.reconfigure ASslow0 @01:14:12.17 AS.seek 0.570ml @01:14:37.98 ASV.dial :wasteD @01:14:38.47 AS.reconfigure ASnorm @01:14:38.50 AS.seek 0.000ml @01:15:07.98 AFV.select :carrierSolution @01:15:08.08 delay 2 seconds @01:15:10.09 AS.reconfigure ASconfig @01:15:10.12 AS.seek 0.500ml @01:15:39.60 delay 1 second @01:15:40.61 AFV.select :holdingCoil @01:15:40.70 ATV.dial ASV @01:15:40.71 ASV.dial :SPErecovery @01:15:41.40 AS.reconfigure ASslow0 @01:15:41.43 AS.seek 0.300ml @01:16:02.10 ATV.dial :air2 @01:16:02.85 AS.reconfigure ASslow1 @01:16:02.88 AS.seek 0.320ml @01:16:07.15 ATV.dial ASV @01:16:07.90 AS.reconfigure ASslow0 @01:16:07.94 AS.seek 0.570ml @01:16:33.71 ASV.dial :wasteD @01:16:34.36 AS.reconfigure ASnorm @01:16:34.39 AS.seek 0.000ml @01:17:03.81 ATV.dial :air2 @01:17:04.53 AS.reconfigure ASslow0 @01:17:04.56 AS.seek 0.300ml @01:17:35.45 ATV.dial :ASV @01:17:36.16 ASV.dial :wasteD @01:17:36.18 AS.reconfigure ASconfig @01:17:36.21 AS.seek 0.050ml @01:17:51.06 ATV.dial :waste @01:17:52.48 AS.seek 0.000ml @01:17:55.64 ASV.dial :SPEdelivery @01:17:56.81 deconLysateINT @01:17:56.82 ATV.dial :air3 @01:17:57.48 AS.reconfigure ASslow1 @01:17:57.52 AS.seek 0.010ml @01:17:59.88 delay 2 seconds @01:18:01.89 ATV.dial :bleach @01:18:02.27 AS.seek 0.960ml @01:21:16.26 delay 3 seconds @01:21:19.27 ATV.dial :air3 @01:21:19.60 delay 3 seconds @01:21:22.66 AS.seek 0.985ml @01:21:28.02 delay 3 seconds @01:21:31.03 ATV.dial :bleach @01:21:31.40 AS.seek 0.970ml @01:21:34.73 delay 3 seconds @01:21:37.74 ATV.dial :AIV @01:21:38.81 AIV.dial :coilToMFB @01:21:38.82 AWV.select :waste @01:21:38.83 AFV.select :carrierSolution @01:21:38.92 delay 2 seconds @01:21:40.93 AS.reconfigure ASconfig @01:21:40.96 AS.seek 1.000ml @01:21:43.04 delay 1 second @01:21:44.05 AFV.select :holdingCoil @01:21:44.14 delay 1 second @01:21:45.15 AS.reconfigure ASslow0 @01:21:45.18 AS.seek empty @01:23:27.62 AFV.select :carrierSolution @01:23:27.72 delay 2 seconds @01:23:29.73 AS.reconfigure ASconfig @01:23:29.76 AS.seek 1.000ml @01:24:28.43 delay 1 second @01:24:29.44 AFV.select :holdingCoil @01:24:29.53 delay 1 second @01:24:30.54 AS.reconfigure ASslow0 @01:24:30.57 AS.seek empty @01:26:12.96 AFV.select :carrierSolution @01:26:13.05 delay 2 seconds @01:26:15.06 AS.reconfigure ASconfig @01:26:15.09 AS.seek 1.000ml @01:27:13.74 delay 1 second @01:27:14.75 AFV.select :holdingCoil @01:27:14.84 delay 1 second @01:27:15.85 AS.reconfigure ASslow0 @01:27:15.88 AS.seek empty @01:28:58.30 ATV.dial :air2 @01:28:58.87 AS.reconfigure ASslow1 @01:28:58.90 AS.seek 0.009ml @01:29:01.24 AS.reconfigure ASslow0 @01:29:01.28 AS.seek 1.000ml @01:30:42.62 ATV.dial AIV @01:30:43.26 AS.seek 0.020ml @01:32:23.54 ATV.dial :waste @01:32:24.79 AS.seek 0.000ml @01:32:27.12 AWV.select :air @01:32:27.26 Cleaning PCR @01:32:27.27 AFV.select :carrierSolution @01:32:27.36 delay 2 seconds @01:32:29.37 AS.reconfigure ASconfig @01:32:29.40 AS.seek 0.500ml @01:32:58.82 delay 1 second @01:32:59.83 AFV.select :holdingCoil @01:32:59.92 ASV.dial :waste1 @01:33:00.32 ATV.dial :air3 @01:33:00.96 AS.reconfigure ASslow1 @01:33:01.00 AS.seek 0.510ml @01:33:03.32 delay 2 seconds @01:33:05.32 ATV.dial :bleach @01:33:05.70 AS.seek 0.810ml @01:34:07.15 delay 3 seconds @01:34:10.16 ATV.dial :air3 @01:34:10.49 delay 3 seconds @01:34:13.55 AS.seek 0.835ml @01:34:18.91 delay 3 seconds @01:34:21.92 ATV.dial :bleach @01:34:22.81 AS.seek 0.820ml @01:34:26.15 delay 3 seconds @01:34:29.16 ARV.dial :waste @01:34:29.66 delay 3 seconds @01:34:32.67 ATV.dial :ARV @01:34:33.49 AS.reconfigure ASconfig @01:34:33.52 AS.seek 0.470ml @01:34:54.19 AFV.select :carrierSolution @01:34:54.29 delay 2 seconds @01:34:56.30 AS.seek 1.000ml @01:35:27.54 delay 1 second @01:35:28.55 AFV.select :holdingCoil @01:35:28.64 delay 1 second @01:35:29.65 AS.reconfigure ASfast @01:35:29.68 AS.seek 0.000ml @01:35:57.41 AFV.select :carrierSolution @01:35:57.51 delay 2 seconds @01:35:59.52 AS.reconfigure ASconfig @01:35:59.55 AS.seek 1.000ml @01:36:58.14 delay 1 second @01:36:59.15 AFV.select :holdingCoil @01:36:59.24 delay 1 second @01:37:00.25 AS.reconfigure ASfast @01:37:00.28 AS.seek 0.000ml @01:37:28.02 AFV.select :carrierSolution @01:37:28.12 delay 2 seconds @01:37:30.13 AS.reconfigure ASconfig @01:37:30.16 AS.seek 1.000ml @01:38:28.76 delay 1 second @01:38:29.77 AFV.select :holdingCoil @01:38:29.86 delay 1 second @01:38:30.87 AS.reconfigure ASfast @01:38:30.91 AS.seek 0.000ml @01:38:58.67 ARV.dial between carrier and air1 @01:38:59.08 ATV.dial :air3 @01:39:00.01 AS.reconfigure ASslow1 @01:39:00.05 AS.seek 0.010ml @01:39:02.42 delay 2 seconds @01:39:04.43 ATV.dial :bleach @01:39:04.81 AS.seek 0.810ml @01:41:48.25 delay 3 seconds @01:41:51.26 ATV.dial :air3 @01:41:51.58 delay 3 seconds @01:41:54.64 AS.seek 0.835ml @01:41:59.97 delay 3 seconds @01:42:02.98 ATV.dial :bleach @01:42:03.37 AS.seek 0.820ml @01:42:06.69 delay 3 seconds @01:42:09.70 ATV.dial PCR @01:42:10.04 AS.reconfigure ASconfig @01:42:10.07 AS.seek empty @01:42:58.19 PCR.program :bleachProfile @01:42:58.25 PCR program should complete in < 2:45 @01:42:58.26 PCR.run 10:00 @01:45:59.53 PCR.off @01:45:59.56 AFV.select :carrierSolution @01:45:59.65 delay 2 seconds @01:46:01.66 AS.seek 1.000ml @01:47:00.29 delay 1 second @01:47:01.30 AFV.select :holdingCoil @01:47:01.39 delay 1 second @01:47:02.40 AS.reconfigure ASfast @01:47:02.43 AS.seek 0.000ml @01:47:30.16 delay 3 seconds @01:47:33.17 AFV.select :carrierSolution @01:47:33.26 delay 2 seconds @01:47:35.27 AS.reconfigure ASconfig @01:47:35.31 AS.seek 1.000ml @01:48:33.91 delay 1 second @01:48:34.92 AFV.select :holdingCoil @01:48:35.01 delay 1 second @01:48:36.02 AS.reconfigure ASfast @01:48:36.05 AS.seek 0.000ml @01:49:03.78 delay 3 seconds @01:49:06.79 AFV.select :carrierSolution @01:49:06.88 delay 2 seconds @01:49:08.89 AS.reconfigure ASconfig @01:49:08.92 AS.seek 1.000ml @01:50:07.53 delay 1 second @01:50:08.54 AFV.select :holdingCoil @01:50:08.63 delay 1 second @01:50:09.64 AS.reconfigure ASfast @01:50:09.67 AS.seek 0.000ml @01:50:37.37 delay 3 seconds @01:50:40.38 AFV.select :carrierSolution @01:50:40.47 delay 2 seconds @01:50:42.48 AS.reconfigure ASconfig @01:50:42.51 AS.seek 1.000ml @01:51:41.07 delay 1 second @01:51:42.08 AFV.select :holdingCoil @01:51:42.17 delay 1 second @01:51:43.18 AS.reconfigure ASfast @01:51:43.21 AS.seek 0.000ml @01:52:10.93 delay 3 seconds @01:52:13.94 AFV.select :carrierSolution @01:52:14.03 delay 2 seconds @01:52:16.04 AS.reconfigure ASconfig @01:52:16.07 AS.seek 1.000ml @01:53:14.70 delay 1 second @01:53:15.71 AFV.select :holdingCoil @01:53:15.80 delay 1 second @01:53:16.81 AS.reconfigure ASnorm @01:53:16.84 AS.seek 0.000ml @01:54:08.28 AFV.select :carrierSolution @01:54:08.37 delay 2 seconds @01:54:10.38 AS.reconfigure ASconfig @01:54:10.41 AS.seek 1.000ml @01:55:09.02 delay 1 second @01:55:10.03 AFV.select :holdingCoil @01:55:10.12 delay 1 second @01:55:11.13 ATV.dial :waste @01:55:12.03 AS.reconfigure ASnorm @01:55:12.06 AS.seek 0.500ml @01:55:37.97 ATV.dial :waste2 @01:55:39.04 AS.seek 0.000ml @01:56:04.94
Completed {wqPCR} begun 18:28:53 @01:56:04.95 Asleep after completing 14mar02sccwrp assays @01:56:04.97 GoodNews.email "neo completed 14mar02sccwrp mission",Subject => "Ready to Recover" @01:56:04.98 GoodNews.email "",Subject => "Asleep after completing 14mar02sccwrp assays" @01:56:05.01 SocketError in MAIN -- getaddrinfo: Temporary failure in name resolution @01:56:05.02 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @01:56:05.04
Retry #1 of 10 after 5:00 @01:56:05.05 Retry #1 of 10 after 5:00 @02:01:05.05
GoodNews.email "neo completed 14mar02sccwrp mission",Subject => "Ready to Recover" @02:01:05.07 GoodNews.email "",Subject => "Asleep after completing 14mar02sccwrp assays" @02:01:05.09 SocketError in MAIN -- getaddrinfo: Temporary failure in name resolution @02:01:05.11 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @02:01:05.12
Retry #2 of 10 after 5:00 @02:01:05.13 Retry #2 of 10 after 5:00 @02:06:05.13
GoodNews.email "neo completed 14mar02sccwrp mission",Subject => "Ready to Recover" @02:06:05.15 GoodNews.email "",Subject => "Asleep after completing 14mar02sccwrp assays" @02:06:05.17 SocketError in MAIN -- getaddrinfo: Temporary failure in name resolution @02:06:05.18 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @02:06:05.20
Retry #3 of 10 after 5:00 @02:06:05.21 Retry #3 of 10 after 5:00 @02:11:05.20
GoodNews.email "neo completed 14mar02sccwrp mission",Subject => "Ready to Recover" @02:11:05.66 GoodNews.email "",Subject => "Asleep after completing 14mar02sccwrp assays" @02:11:05.68 SocketError in MAIN -- getaddrinfo: Temporary failure in name resolution @02:11:05.69 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @02:11:05.71
Retry #4 of 10 after 5:00 @02:11:05.72 Retry #4 of 10 after 5:00 @02:16:05.72
GoodNews.email "neo completed 14mar02sccwrp mission",Subject => "Ready to Recover" @02:16:05.74 GoodNews.email "",Subject => "Asleep after completing 14mar02sccwrp assays" @02:16:05.76 SocketError in MAIN -- getaddrinfo: Temporary failure in name resolution @02:16:05.78 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @02:16:05.79
Retry #5 of 10 after 5:00 @02:16:05.80 Retry #5 of 10 after 5:00 @02:21:05.80
GoodNews.email "neo completed 14mar02sccwrp mission",Subject => "Ready to Recover" @02:21:05.82 GoodNews.email "",Subject => "Asleep after completing 14mar02sccwrp assays" @02:21:05.84 SocketError in MAIN -- getaddrinfo: Temporary failure in name resolution @02:21:05.86 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @02:21:05.87
Retry #6 of 10 after 5:00 @02:21:05.88 Retry #6 of 10 after 5:00 @02:26:05.88
GoodNews.email "neo completed 14mar02sccwrp mission",Subject => "Ready to Recover" @02:26:05.90 GoodNews.email "",Subject => "Asleep after completing 14mar02sccwrp assays" @02:26:05.92 SocketError in MAIN -- getaddrinfo: Temporary failure in name resolution @02:26:05.94 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @02:26:05.95
Retry #7 of 10 after 5:00 @02:26:05.96 Retry #7 of 10 after 5:00 @02:31:05.96
GoodNews.email "neo completed 14mar02sccwrp mission",Subject => "Ready to Recover" @02:31:05.98 GoodNews.email "",Subject => "Asleep after completing 14mar02sccwrp assays" @02:31:06.00 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @02:31:06.02 SocketError in MAIN -- getaddrinfo: Temporary failure in name resolution @02:31:06.03 Retry #8 of 10 after 5:00 @02:31:06.04
Retry #8 of 10 after 5:00 @02:36:06.05 GoodNews.email "",Subject => "Asleep after completing 14mar02sccwrp assays" @02:36:06.07 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @02:36:06.08 Retry #9 of 10 after 5:00 @02:36:06.09
GoodNews.email "neo completed 14mar02sccwrp mission",Subject => "Ready to Recover" @02:36:06.11 SocketError in MAIN -- getaddrinfo: Temporary failure in name resolution @02:36:06.12 Retry #9 of 10 after 5:00 @02:41:06.08 GoodNews.email "",Subject => "Asleep after completing 14mar02sccwrp assays" @02:41:06.10 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @02:41:06.11 Retry #10 of 10 after 5:00 @02:41:06.12
GoodNews.email "neo completed 14mar02sccwrp mission",Subject => "Ready to Recover" @02:41:06.14 SocketError in MAIN -- getaddrinfo: Temporary failure in name resolution @02:41:06.15 Retry #10 of 10 after 5:00 @02:46:06.12 GoodNews.email "",Subject => "Asleep after completing 14mar02sccwrp assays" @02:46:06.14 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @02:46:06.16
GoodNews.email "neo completed 14mar02sccwrp mission",Subject => "Ready to Recover" @02:46:06.18 SocketError in MAIN -- getaddrinfo: Temporary failure in name resolution @02:46:06.19 Waiting to Resume from Checkpoint "Garaging FlushPuck in Collection Clamp" @02:46:06.20 BadNews.email "getaddrinfo: Temporary failure in name resolution",Subject => "SocketError in MAIN" @02:46:06.22 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @02:46:06.23 Retry #1 of 10 after 5:00 @02:51:06.24 BadNews.email "getaddrinfo: Temporary failure in name resolution",Subject => "SocketError in MAIN" @02:51:06.26 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @02:51:06.27 Retry #2 of 10 after 5:00 @02:56:06.27 BadNews.email "getaddrinfo: Temporary failure in name resolution",Subject => "SocketError in MAIN" @02:56:06.29 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @02:56:06.30 Retry #3 of 10 after 5:00 @03:01:06.30 BadNews.email "getaddrinfo: Temporary failure in name resolution",Subject => "SocketError in MAIN" @03:01:06.32 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @03:01:06.33 Retry #4 of 10 after 5:00 @03:06:06.34 BadNews.email "getaddrinfo: Temporary failure in name resolution",Subject => "SocketError in MAIN" @03:06:06.36 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @03:06:06.37 Retry #5 of 10 after 5:00 @03:11:06.37 BadNews.email "getaddrinfo: Temporary failure in name resolution",Subject => "SocketError in MAIN" @03:11:06.39 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @03:11:06.40 Retry #6 of 10 after 5:00 @03:16:06.40 BadNews.email "getaddrinfo: Temporary failure in name resolution",Subject => "SocketError in MAIN" @03:16:06.42 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @03:16:06.43 Retry #7 of 10 after 5:00 @03:21:06.43 BadNews.email "getaddrinfo: Temporary failure in name resolution",Subject => "SocketError in MAIN" @03:21:06.45 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @03:21:06.46 Retry #8 of 10 after 5:00 @03:26:06.47 BadNews.email "getaddrinfo: Temporary failure in name resolution",Subject => "SocketError in MAIN" @03:26:06.49 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @03:26:06.50 Retry #9 of 10 after 5:00 @03:31:06.50 BadNews.email "getaddrinfo: Temporary failure in name resolution",Subject => "SocketError in MAIN" @03:31:06.52 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @03:31:06.53 Retry #10 of 10 after 5:00 @03:36:06.53 BadNews.email "getaddrinfo: Temporary failure in name resolution",Subject => "SocketError in MAIN" @03:36:06.55 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @06:50:26.88 Session Begun @06:51:19.96 -> showlog @06:52:03.73 -> showlog @06:52:41.11 -> Hello Blythe @06:52:41.12 NameError in ramu -- uninitialized constant Blythe @06:52:51.83 -> exit @06:52:51.84 Session Ended @15:44:01.69 Session Begun @15:44:01.72 -> Sleepy.cycleModemPower! 9 @15:44:01.74 Session Ended @08:29:47.23PST05-Mar-14 ESP2 v3.20 I2C Gateway v3.01 @08:29:47.58 Powering up Dwarves @08:29:53.95
Configuration for neo by maro@mbari.org /home/esp/esp2/shallow/neo/configure.rb @08:29:54.05 Configuration for MFB10 by preston@mbari.org /home/esp/esp2/mfb/MFB10/configure.rb @08:29:57.47 Sleepy.power :raw,:ON @08:29:58.18 SPE.coast @08:29:58.22 SPE.configure SPEconfig @08:29:58.25 AS.stop @08:29:58.28 AS.configure ASconfig @08:29:58.31 CC.stop @08:29:58.33 CC.configure CCconfig @08:29:58.36 CS.stop @08:29:58.38 CS.configure CSconfig @08:29:58.41 PC.stop @08:29:58.43 PC.configure PCconfig @08:29:58.46 PS.stop @08:29:58.48 PS.configure PSconfig @08:29:58.54 Forearm.stop @08:29:58.56 Forearm.configure LooseForearm @08:29:58.59 Elbow.stop @08:29:58.61 Elbow.configure LooseElbow @08:29:58.68 SC.stop @08:29:58.70 SC.configure SCloose @08:29:58.73 SE.stop @08:29:58.75 SE.configure SEconfig @08:29:58.78 SS.stop @08:29:58.80 SS.configure SSconfig @08:29:58.83 CH.coast @08:29:58.87 CH.configure CHconfig @08:29:58.90 PH.coast @08:29:58.94 PH.configure PHconfig @08:29:58.98 PCR.off @08:29:59.01 PCR.pidtemp= 50,0.1,0,300,25,25 @08:29:59.04 PCR.caltemp= 6.6661,0.0344,0.0 @08:29:59.07 All dwarves are running firmware version 3.74 @08:29:59.63 GoodNews.email "Starting w/pucks in tube 2\n ",Subject => "14mar05sccwrpDeploy begun" @08:30:00.49
Asleep til +0:10 @08:30:00.52 Sleeping until 08:30 @08:30:00.55 Sleepy.power :raw,:OFF @08:30:00.56 Sleepy.power :dwarf,:OFF Shutdown @08:30:08.61 GoodNews.email "Then, in tube 2: wqPCR\n/home/esp/esp2local/mission/14mar05sccwrpDeploy.rb:55",Subject => "Asleep until 08:30" @08:30:09.84
Powering up Dwarves @08:30:14.99 Sleepy.power :raw,:ON @08:30:15.47 SPE.configure SPEconfig @08:30:15.49 AS.configure ASconfig @08:30:15.53 CC.configure CCconfig @08:30:15.56 CS.configure CSconfig @08:30:15.59 PC.configure PCconfig @08:30:15.62 PS.configure PSconfig @08:30:15.65 Forearm.configure LooseForearm @08:30:15.68 Elbow.configure LooseElbow @08:30:15.74 SC.configure SCloose @08:30:15.77 SE.configure SEconfig @08:30:15.81 SS.configure SSconfig @08:30:15.84 CH.configure CHconfig @08:30:15.86 PH.configure PHconfig @08:30:15.89 Valve.reset @08:30:16.49 PCR.off @08:30:16.52 PCR.pidtemp= 50,0.1,0,300,25,25 @08:30:16.55 PCR.caltemp= 6.6661,0.0344,0.0 @08:30:16.64 Intake.select :close @08:30:16.68 Exhaust.select :close CTV.dial :air @08:30:16.72 PSV.dial :air @08:30:16.75
PC.reconfigure PCconfig @08:30:16.82 PC.home.seek open @08:30:16.88 CSV.dial :waste1 @08:30:17.23 PS.reconfigure PSconfig @08:30:17.26 PS.home.seek 0.000ml @08:30:17.31 SSV.dial :spit,avoiding => exhaust @08:30:18.11 CS.reconfigure CSfast @08:30:18.14 SS.reconfigure SSconfig @08:30:18.23 SS.home.seek 0.00ml @08:30:18.25 CS.home.seek 0.000ml @08:30:18.33 CC.reconfigure CCconfig @08:30:18.36 CC.home.seek open @08:30:27.51 PS.coast @08:30:27.59
CC.seek open @08:30:27.61 PC.seek open @08:30:27.76
Forearm.reconfigure LooseForearm @08:30:27.79 Forearm.home.seek retract @08:30:28.54 Hand.grip @08:30:28.56
Elbow.jog -1500 @08:30:29.11 Elbow.reconfigure LooseElbow @08:30:29.15 Elbow.home.seek clear @08:30:33.88 SE.reconfigure SEconfig @08:30:33.91 SE.home.seek down @08:30:34.44 SC.reconfigure SCsloppy @08:30:34.48 SC.home.seek initial @08:30:36.71 Forearm.seek retract @08:30:36.76 Elbow.seek hammer @08:30:37.95 Forearm.reconfigure TightForearm @08:30:38.00 Forearm.seek 2 @08:30:39.14 Forearm.seek retract @08:30:40.18 Forearm.reconfigure LooseForearm @08:30:40.59PST05-Mar-14 ===> STARTED wqPCR (phase #2) <=== @08:30:40.60 Starting initialPurge @08:30:40.61 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:30:40.66 Hand.release @08:30:41.43 Elbow.reconfigure TightElbow @08:30:41.46 Elbow.seek FlushPuck::Garage @08:30:43.22 Elbow.reconfigure LooseElbow @08:30:43.31 Forearm.seek garage @08:30:44.32 Hand.grip @08:30:45.19 Forearm.seek retract @08:30:46.20 Elbow.reconfigure TightElbow @08:30:46.24 Elbow.seek CC @08:30:49.00 Elbow.reconfigure LooseElbow @08:30:49.09 Forearm.reconfigure TightForearm @08:30:49.12 Forearm.seek CC @08:30:50.23 CC.seek closed @08:30:54.00 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.94 @08:30:54.05 Hand.release @08:30:54.89 Forearm.reconfigure LooseForearm @08:30:54.92 Forearm.seek retract @08:30:55.85 Flushing the Collection Valve::Manifold @08:30:55.94 CS Pulling 5ml of flush @08:30:55.95 CSV.dial CSR @08:30:57.22 CSR.select :flush @08:30:57.32 CS.reconfigure CSconfig @08:30:57.35 CS.seek 5.000ml @08:31:10.83 delay 5 seconds @08:31:15.84 CSR.select :air @08:31:15.98 CS.reconfigure CSfast @08:31:16.01 CS.seek 7.500ml @08:31:22.61 CSV.dial :waste @08:31:23.87 CS.reconfigure CSconfig @08:31:23.91 CS.seek 0.000ml @08:31:43.70 Purging external Intake @08:31:43.79 CSV.dial :flush @08:31:44.23 CS.reconfigure CSfast @08:31:44.26 CS.seek 10.000ml @08:32:08.17 delay 5 seconds @08:32:13.18 CSV.dial CBV @08:32:13.20
CBV.dial CSV @08:32:14.00 CTV.dial :intake @08:32:14.70 Intake.select :open @08:32:14.79 CS.seek 0.000ml @08:32:39.11 Intake.select :close @08:32:39.20 CTV.dial :air @08:32:39.95 Flushing upper manifold @08:32:40.04 CSV.dial :flush @08:32:40.83 CS.seek 5.000ml @08:32:53.20 delay 5 seconds @08:32:58.21 CSV.dial :air @08:32:58.69 CS.seek 7.000ml @08:33:04.07 CSV.dial CBV @08:33:04.09
CBV.dial CSV @08:33:05.17 CTV.dial :air @08:33:05.19 CS.seek 0.000ml @08:33:22.04 delay 7 seconds @08:33:29.06 CS.seek 3.000ml @08:33:36.91 CSV.dial :waste @08:33:37.43 CS.seek 0.000ml @08:33:45.28 CSV.dial :flush @08:33:45.71 CS.seek 3.000ml @08:33:53.45 delay 5 seconds @08:33:58.46 CSV.dial :air @08:33:58.93 CS.seek 4.999ml @08:34:04.35 CSV.dial :air @08:34:04.36 CS.seek 0.000ml @08:34:16.73 delay 7 seconds @08:34:23.74 CS.seek 3.000ml @08:34:31.44 CSV.dial :waste @08:34:32.24 CS.seek 0.000ml @08:34:40.01 Garaging FlushPuck in Collection Clamp @08:34:40.07 Elbow.reconfigure TightElbow @08:34:40.10 Elbow.seek CC @08:34:40.29 Elbow.reconfigure LooseElbow @08:34:40.38 Forearm.seek CC @08:34:41.34 CC.seek sealed @08:34:42.29 Hand.grip @08:34:42.96 CC.seek open @08:34:46.25 Forearm.seek retract @08:34:47.19 Elbow.reconfigure TightElbow @08:34:47.23 Elbow.seek garage @08:34:50.03 Elbow.reconfigure LooseElbow @08:34:50.12 Forearm.reconfigure TightForearm @08:34:50.15 Forearm.seek garage @08:34:51.33 Hand.release @08:34:52.13 Forearm.reconfigure LooseForearm @08:34:52.16 Forearm.seek retract @08:34:53.36 Starting mfb @08:34:53.37 pcrBAC sampling at most 1000ml, wcr at most 1000ml @08:34:53.39 Loading sh1 puck from tube 2 into Collection Clamp @08:34:53.42 BACfans.email "Can@08:30:40, 15.9C, 59% humidity, 14.2psia, 13.709V, 0.665A, 0.652A avg, 9.11W\nCTD data unavailable\nISUS data unavailable",Subject => "pcrBAC sampling at most 1000ml, wcr at most 1000ml" @08:34:54.45 Readying the MFB @08:34:54.46 Homing MFB Syringe @08:34:54.49 AFV.select :holdingCoil @08:34:54.72
SC.reconfigure SCloose @08:34:54.76 ATV.dial :air2 @08:34:54.94
Elbow.reconfigure TightElbow @08:34:54.97 Elbow.seek 2 @08:34:55.16 AS.reconfigure ASconfig @08:34:55.19 AS.home.seek home @08:34:57.45
Forearm.reconfigure TightForearm @08:34:57.48 Forearm.seek 2 + 800 counts @08:34:58.46 SE.reconfigure SEpick @08:34:58.52 SE.seek beamBreak @08:34:59.72 SE.configure SEconfig @08:34:59.85 Elbow.seek 2 @08:35:00.00 Forearm.seek 2 + 300 counts @08:35:00.27 Forearm.reconfigure LooseForearm @08:35:00.31 Hand.grip @08:35:01.11 SE.seek down @08:35:01.24
Forearm.seek retract @08:35:02.10 SE.coast @08:35:02.21
Elbow.seek CC @08:35:02.68 AFV.select :carrierSolution @08:35:02.79 delay 2 seconds @08:35:03.72
Elbow.reconfigure LooseElbow @08:35:03.80 Forearm.reconfigure TightForearm @08:35:03.83 Forearm.seek CC @08:35:04.80 AS.seek 1.000ml @08:35:04.98
CC.seek closed @08:35:08.74 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.95 @08:35:08.75 Hand.release @08:35:09.50 Forearm.reconfigure LooseForearm @08:35:09.53 Forearm.seek retract @08:35:10.53 SC.coast @08:35:10.56 collectSample 1000ml w/bubble point of 55psi @08:35:10.57 Priming 7ml Sleepy.power :sampler,:ON @08:35:10.58 Intake.select :close Exhaust.select :close @08:35:10.59 SS.hold @08:35:10.76 Calibrating pressure and temperature sensors @08:35:10.77 CTV.dial :air @08:35:10.78 SSV.dial :spit,avoiding => exhaust @08:35:10.80 SS.reconfigure SSfast @08:35:10.83 SS.seek 5.00ml @08:35:13.74 SSV.dial CBV @08:35:13.76
CBV.dial SSV @08:35:15.12 delay 3.8 seconds @08:35:19.00 SSV.dial :spit @08:35:20.34 SS.seek 0.00ml @08:35:22.97 SSV.dial CBV @08:35:24.30 CTV.dial :intake @08:35:25.07 Waiting for external sampler to finish priming... @08:35:25.08 delayUntil "08:45:10.57" @08:35:56.52 delay 1 second @08:35:57.53 AFV.select :holdingCoil @08:35:57.62 delay 1 second @08:35:58.63 ATV.dial :waste @08:35:59.54 AS.reconfigure ASnorm @08:35:59.57 AS.home.seek empty @08:36:52.75 ATV.dial :waste @08:36:52.76 ARV.dial between carrier and air1 @08:36:52.83 ASV.dial :waste1 @08:45:10.57 SSV.dial CBV @08:45:10.59
CBV.dial SSV Allowing 55s for 7.0ml of remaining 7.0ml @08:45:10.60 Intake.select :open @08:45:12.74 Intake @34.2psia, vacuum from 20.0 to 24.0psi @08:45:12.75 SS.reconfigure forSampling @08:45:12.79 SS.seek 7.00ml @08:45:18.44 Stroke completed -- waiting for pressure to equalize ... @08:45:19.07 Intake.select :close @08:45:19.21 SSV.dial :exhaust @08:45:19.97 Exhaust.select :open @08:45:20.06 SS.reconfigure SSexhale @08:45:20.10 SS.seek 0.00ml @08:45:23.94 Exhaust.select :close @08:45:24.04 Sampling 1000.0ml @08:45:24.05 Intake.select :close @08:45:24.06 Exhaust.select :close @08:45:24.07 SS.reconfigure SSconfig @08:45:24.10 SS.hold @08:45:24.25 CTV.dial :intake @08:45:24.26 SSV.dial CBV @08:45:24.28
CBV.dial SSV @08:45:25.06 Allowing 185s for 25.0ml of remaining 1000.0ml Intake.select :open @08:45:27.19 Intake @35.9psia, vacuum from 20.0 to 24.0psi @08:45:27.20 SS.reconfigure forSampling @08:45:27.24 SS.seek 25.00ml @08:45:41.55 Stroke completed -- waiting for pressure to equalize ... @08:45:42.19 Intake.select :close @08:45:42.32 SSV.dial :exhaust @08:45:43.10 Exhaust.select :open @08:45:43.19 SS.reconfigure SSexhale @08:45:43.23 SS.seek 0.00ml @08:45:52.09 Exhaust.select :close @08:45:52.18 SSV.dial CBV @08:45:52.20
CBV.dial SSV @08:45:52.99 Allowing 185s for 25.0ml of remaining 975.0ml @08:45:53.00 Intake.select :open @08:45:55.13 Intake @35.4psia, vacuum from 20.0 to 24.0psi @08:45:55.14 SS.reconfigure forSampling @08:45:55.18 SS.seek 25.00ml @08:46:09.48 Stroke completed -- waiting for pressure to equalize ... @08:46:10.12 Intake.select :close @08:46:10.25 SSV.dial :exhaust @08:46:11.03 Exhaust.select :open @08:46:11.13 SS.reconfigure SSexhale @08:46:11.17 SS.seek 0.00ml @08:46:20.10 Exhaust.select :close @08:46:20.19 SSV.dial CBV @08:46:20.21
CBV.dial SSV @08:46:20.99 Allowing 185s for 25.0ml of remaining 950.0ml @08:46:21.00 Intake.select :open @08:46:23.13 Intake @34.4psia, vacuum from 20.0 to 24.0psi @08:46:23.14 SS.reconfigure forSampling @08:46:23.18 SS.seek 25.00ml @08:46:28.74 Vacuum too high -- waiting up to 40s @08:46:29.00 Intake @25.4psia, vacuum from 18.9 to 22.9psi @08:46:29.01 SS.reconfigure forSampling @08:46:29.05 SS.seek 25.00ml @08:46:38.68 Stroke completed -- waiting for pressure to equalize ... @08:46:39.31 Intake.select :close @08:46:39.45 SSV.dial :exhaust @08:46:40.23 Exhaust.select :open @08:46:40.88 SS.reconfigure SSexhale @08:46:40.92 SS.seek 0.00ml @08:46:49.68 Exhaust.select :close @08:46:49.77 SSV.dial CBV @08:46:49.79
CBV.dial SSV @08:46:50.59 Allowing 185s for 25.0ml of remaining 925.0ml @08:46:50.60 Intake.select :open @08:46:52.73 Intake @33.6psia, vacuum from 20.0 to 24.0psi @08:46:52.74 SS.reconfigure forSampling @08:46:52.78 SS.seek 25.00ml @08:46:56.75 Vacuum too high -- waiting up to 40s @08:46:57.01 Intake @26.6psia, vacuum from 20.0 to 24.0psi @08:46:57.02 SS.reconfigure forSampling @08:46:57.05 SS.seek 25.00ml @08:47:10.10 Stroke completed -- waiting for pressure to equalize ... @08:47:10.74 Intake.select :close @08:47:10.88 SSV.dial :exhaust @08:47:11.66 Exhaust.select :open @08:47:11.75 SS.reconfigure SSexhale @08:47:11.79 SS.seek 0.00ml @08:47:20.82 Exhaust.select :close @08:47:20.91 SSV.dial CBV @08:47:20.93
CBV.dial SSV @08:47:21.72 Allowing 185s for 25.0ml of remaining 900.0ml @08:47:21.73 Intake.select :open @08:47:23.86 Intake @33.9psia, vacuum from 20.0 to 24.0psi @08:47:23.87 SS.reconfigure forSampling @08:47:23.91 SS.seek 25.00ml @08:47:26.75 Vacuum too high -- waiting up to 40s @08:47:27.01 Intake @28.5psia, vacuum from 20.0 to 24.0psi @08:47:27.03 SS.reconfigure forSampling @08:47:27.06 SS.seek 25.00ml @08:47:33.67 Vacuum too high -- waiting up to 40s @08:47:34.16 Intake @30.0psia, vacuum from 20.0 to 24.0psi @08:47:34.17 SS.reconfigure forSampling @08:47:34.20 SS.seek 25.00ml @08:47:38.64 Vacuum too high -- waiting up to 40s @08:47:39.12 Intake @30.2psia, vacuum from 20.0 to 24.0psi @08:47:39.13 SS.reconfigure forSampling @08:47:39.17 SS.seek 25.00ml @08:47:48.49 Stroke completed -- waiting for pressure to equalize ... @08:47:49.13 Intake.select :close @08:47:49.27 SSV.dial :exhaust @08:47:50.26 Exhaust.select :open @08:47:50.35 SS.reconfigure SSexhale @08:47:50.39 SS.seek 0.00ml @08:47:59.07 Exhaust.select :close @08:47:59.16 SSV.dial CBV @08:47:59.18
CBV.dial SSV @08:47:59.98 Allowing 185s for 25.0ml of remaining 875.0ml Intake.select :open @08:48:02.11 Intake @33.9psia, vacuum from 20.0 to 24.0psi @08:48:02.12 SS.reconfigure forSampling @08:48:02.16 SS.seek 25.00ml @08:48:04.59 Vacuum too high -- waiting up to 40s @08:48:04.85 Intake @31.6psia, vacuum from 20.0 to 24.0psi @08:48:04.86 SS.reconfigure forSampling @08:48:04.89 SS.seek 25.00ml @08:48:15.86 Vacuum too high -- waiting up to 40s @08:48:16.33 Intake @32.2psia, vacuum from 20.0 to 24.0psi @08:48:16.34 SS.reconfigure forSampling @08:48:16.38 SS.seek 25.00ml @08:48:31.98 Vacuum too high -- waiting up to 40s @08:48:32.24 Intake @30.6psia, vacuum from 20.0 to 24.0psi @08:48:32.25 SS.reconfigure forSampling @08:48:32.29 SS.seek 25.00ml @08:48:43.17 Stroke completed -- waiting for pressure to equalize ... @08:48:43.80 Intake.select :close @08:48:43.94 SSV.dial :exhaust @08:48:44.72 Exhaust.select :open @08:48:44.81 SS.reconfigure SSexhale @08:48:44.85 SS.seek 0.00ml @08:48:53.45 Exhaust.select :close @08:48:53.54 SSV.dial CBV @08:48:53.56
CBV.dial SSV @08:48:54.36 Allowing 185s for 25.0ml of remaining 850.0ml @08:48:54.37 Intake.select :open @08:48:56.50 Intake @32.3psia, vacuum from 20.0 to 24.0psi @08:48:56.51 SS.reconfigure forSampling @08:48:56.55 SS.seek 25.00ml @08:49:41.51 Vacuum too high -- waiting up to 40s @08:49:41.77 Intake @30.7psia, vacuum from 20.0 to 24.0psi @08:49:41.78 SS.reconfigure forSampling @08:49:41.82 SS.seek 25.00ml @08:50:08.62 Stroke completed -- waiting for pressure to equalize ... @08:50:09.84 Intake.select :close @08:50:09.97 SSV.dial :exhaust @08:50:10.76 Exhaust.select :open @08:50:10.85 SS.reconfigure SSexhale @08:50:10.89 SS.seek 0.00ml @08:50:19.51 Exhaust.select :close @08:50:19.60 SSV.dial CBV @08:50:19.62
CBV.dial SSV @08:50:20.43 Allowing 185s for 25.0ml of remaining 825.0ml @08:50:20.44 Intake.select :open @08:50:22.57 Intake @31.4psia, vacuum from 20.0 to 24.0psi @08:50:22.58 SS.reconfigure forSampling @08:50:22.62 SS.seek 25.00ml @08:52:12.99 Stroke completed -- waiting for pressure to equalize ... @08:52:14.20 Intake.select :close @08:52:14.33 SSV.dial :exhaust @08:52:15.13 Exhaust.select :open @08:52:15.22 SS.reconfigure SSexhale @08:52:15.26 SS.seek 0.00ml @08:52:24.01 Exhaust.select :close @08:52:24.10 SSV.dial CBV @08:52:24.12
CBV.dial SSV @08:52:24.93 Allowing 185s for 25.0ml of remaining 800.0ml @08:52:24.94 Intake.select :open @08:52:27.07 Intake @32.3psia, vacuum from 20.0 to 24.0psi @08:52:27.08 SS.reconfigure forSampling @08:52:27.11 SS.seek 25.00ml @08:54:17.41 Stroke completed -- waiting for pressure to equalize ... @08:54:19.20 Intake.select :close @08:54:19.34 SSV.dial :exhaust @08:54:20.14 Exhaust.select :open @08:54:20.23 SS.reconfigure SSexhale @08:54:20.27 SS.seek 0.00ml @08:54:28.92 Exhaust.select :close @08:54:29.01 SSV.dial CBV @08:54:29.03
CBV.dial SSV @08:54:29.83 Allowing 185s for 25.0ml of remaining 775.0ml @08:54:29.84 Intake.select :open @08:54:31.97 Intake @37.0psia, vacuum from 20.0 to 24.0psi @08:54:31.98 SS.reconfigure forSampling @08:54:32.02 SS.seek 25.00ml @08:54:39.62 Vacuum too high -- waiting up to 40s @08:54:39.88 Intake @35.7psia, vacuum from 20.0 to 24.0psi @08:54:39.89 SS.reconfigure forSampling @08:54:39.93 SS.seek 25.00ml @08:55:05.18 Vacuum too high -- waiting up to 40s @08:55:05.65 Intake @36.3psia, vacuum from 20.0 to 24.0psi @08:55:05.66 SS.reconfigure forSampling @08:55:05.70 SS.seek 25.00ml @08:55:08.66 Vacuum too high -- waiting up to 40s @08:55:08.92 Intake @36.2psia, vacuum from 20.0 to 24.0psi @08:55:08.93 SS.reconfigure forSampling @08:55:08.96 SS.seek 25.00ml @08:55:11.10 Vacuum too high -- waiting up to 40s @08:55:11.35 Intake @35.9psia, vacuum from 20.0 to 24.0psi @08:55:11.36 SS.reconfigure forSampling @08:55:11.40 SS.seek 25.00ml @08:55:14.29 Vacuum too high -- waiting up to 40s @08:55:14.56 Intake @36.5psia, vacuum from 20.0 to 24.0psi @08:55:14.57 SS.reconfigure forSampling @08:55:14.60 SS.seek 25.00ml @08:55:17.93 Vacuum too high -- waiting up to 40s @08:55:18.20 Intake @36.9psia, vacuum from 20.0 to 24.0psi @08:55:18.21 SS.reconfigure forSampling @08:55:18.24 SS.seek 25.00ml @08:55:20.13 Vacuum too high -- waiting up to 40s @08:55:20.39 Intake @36.9psia, vacuum from 20.0 to 24.0psi @08:55:20.40 SS.reconfigure forSampling @08:55:20.43 SS.seek 25.00ml @08:55:22.57 Vacuum too high -- waiting up to 40s @08:55:22.83 Intake @36.3psia, vacuum from 20.0 to 24.0psi @08:55:22.84 SS.reconfigure forSampling @08:55:22.87 SS.seek 25.00ml @08:55:24.60 Vacuum too high -- waiting up to 40s @08:55:24.87 SS.seek 25.00ml @08:55:26.69 Vacuum too high -- waiting up to 40s @08:55:26.95 Intake @36.4psia, vacuum from 20.0 to 24.0psi @08:55:26.96 SS.reconfigure forSampling @08:55:26.99 SS.seek 25.00ml @08:55:28.69 Vacuum too high -- waiting up to 40s @08:55:28.95 Intake @36.2psia, vacuum from 20.0 to 24.0psi @08:55:28.96 SS.reconfigure forSampling @08:55:28.99 SS.seek 25.00ml @08:55:30.85 Vacuum too high -- waiting up to 40s @08:55:31.31 Intake @36.9psia, vacuum from 20.0 to 24.0psi @08:55:31.32 SS.reconfigure forSampling @08:55:31.36 SS.seek 25.00ml @08:55:33.35 Vacuum too high -- waiting up to 40s @08:55:33.61 Intake @36.5psia, vacuum from 20.0 to 24.0psi @08:55:33.62 SS.reconfigure forSampling @08:55:33.65 SS.seek 25.00ml @08:55:35.56 Vacuum too high -- waiting up to 40s @08:55:35.82 Intake @37.1psia, vacuum from 20.0 to 24.0psi @08:55:35.83 SS.reconfigure forSampling @08:55:35.86 SS.seek 25.00ml @08:55:37.19 Vacuum too high -- waiting up to 40s @08:55:37.45 Intake @36.4psia, vacuum from 20.0 to 24.0psi @08:55:37.46 SS.reconfigure forSampling @08:55:37.49 SS.seek 25.00ml @08:55:39.13 Vacuum too high -- waiting up to 40s @08:55:39.60 Intake @36.8psia, vacuum from 20.0 to 24.0psi @08:55:39.61 SS.reconfigure forSampling @08:55:39.65 SS.seek 25.00ml @08:55:41.66 Vacuum too high -- waiting up to 40s @08:55:41.92 Intake @36.3psia, vacuum from 20.0 to 24.0psi @08:55:41.93 SS.reconfigure forSampling @08:55:41.96 SS.seek 25.00ml @08:55:43.47 Vacuum too high -- waiting up to 40s @08:55:43.72 Intake @36.1psia, vacuum from 20.0 to 24.0psi @08:55:43.73 SS.reconfigure forSampling @08:55:43.77 SS.seek 25.00ml @08:55:45.41 Vacuum too high -- waiting up to 40s @08:55:45.67 Intake @36.2psia, vacuum from 20.0 to 24.0psi @08:55:45.68 SS.reconfigure forSampling @08:55:45.72 SS.seek 25.00ml @08:55:47.28 Vacuum too high -- waiting up to 40s @08:55:47.53 Intake @36.3psia, vacuum from 20.0 to 24.0psi @08:55:47.54 SS.reconfigure forSampling @08:55:47.58 SS.seek 25.00ml @08:55:49.13 Vacuum too high -- waiting up to 40s @08:55:49.39 Intake @36.5psia, vacuum from 20.0 to 24.0psi @08:55:49.40 SS.reconfigure forSampling @08:55:49.43 SS.seek 25.00ml @08:55:50.94 Vacuum too high -- waiting up to 40s @08:55:51.40 Intake @37.4psia, vacuum from 20.0 to 24.0psi @08:55:51.41 SS.reconfigure forSampling @08:55:51.45 SS.seek 25.00ml @08:55:53.19 Vacuum too high -- waiting up to 40s @08:55:53.44 Intake @36.9psia, vacuum from 20.0 to 24.0psi @08:55:53.45 SS.reconfigure forSampling @08:55:53.49 SS.seek 25.00ml @08:55:55.00 Vacuum too high -- waiting up to 40s @08:55:55.47 Intake @36.9psia, vacuum from 20.0 to 24.0psi @08:55:55.48 SS.reconfigure forSampling @08:55:55.51 SS.seek 25.00ml @08:55:57.38 Vacuum too high -- waiting up to 40s @08:55:57.64 Intake @36.9psia, vacuum from 20.0 to 24.0psi @08:55:57.65 SS.reconfigure forSampling @08:55:57.68 SS.seek 25.00ml @08:55:59.00 Vacuum too high -- waiting up to 40s @08:55:59.25 Intake @36.7psia, vacuum from 20.0 to 24.0psi @08:55:59.26 SS.reconfigure forSampling @08:55:59.30 SS.seek 25.00ml @08:56:00.75 Vacuum too high -- waiting up to 40s @08:56:01.00 Intake @36.5psia, vacuum from 20.0 to 24.0psi @08:56:01.01 SS.reconfigure forSampling @08:56:01.05 SS.seek 25.00ml @08:56:02.41 Vacuum too high -- waiting up to 40s @08:56:02.67 Intake @36.3psia, vacuum from 20.0 to 24.0psi @08:56:02.68 SS.reconfigure forSampling @08:56:02.71 SS.seek 25.00ml @08:56:03.97 Vacuum too high -- waiting up to 40s @08:56:04.44 Intake @36.4psia, vacuum from 20.0 to 24.0psi @08:56:04.45 SS.reconfigure forSampling @08:56:04.48 SS.seek 25.00ml @08:56:06.22 Vacuum too high -- waiting up to 40s @08:56:06.68 Intake @36.3psia, vacuum from 20.0 to 24.0psi @08:56:06.69 SS.reconfigure forSampling @08:56:06.73 SS.seek 25.00ml @08:56:08.38 Vacuum too high -- waiting up to 40s @08:56:09.16 Intake @37.5psia, vacuum from 20.0 to 24.0psi @08:56:09.17 SS.reconfigure forSampling @08:56:09.21 SS.seek 25.00ml @08:56:11.25 Vacuum too high -- waiting up to 40s @08:56:11.51 Intake @37.0psia, vacuum from 20.0 to 24.0psi @08:56:11.52 SS.reconfigure forSampling @08:56:11.55 SS.seek 25.00ml @08:56:12.91 Vacuum too high -- waiting up to 40s @08:56:13.17 Intake @36.9psia, vacuum from 20.0 to 24.0psi @08:56:13.18 SS.reconfigure forSampling @08:56:13.21 SS.seek 25.00ml @08:56:14.47 Vacuum too high -- waiting up to 40s @08:56:14.96 Intake @36.6psia, vacuum from 20.0 to 24.0psi @08:56:14.97 SS.reconfigure forSampling @08:56:15.00 SS.seek 25.00ml @08:56:16.66 Vacuum too high -- waiting up to 40s @08:56:16.92 Intake @36.6psia, vacuum from 20.0 to 24.0psi @08:56:16.93 SS.reconfigure forSampling @08:56:16.96 SS.seek 25.00ml @08:56:18.19 Vacuum too high -- waiting up to 40s @08:56:18.66 Intake @36.7psia, vacuum from 20.0 to 24.0psi @08:56:18.67 SS.reconfigure forSampling @08:56:18.70 SS.seek 25.00ml @08:56:20.25 Vacuum too high -- waiting up to 40s @08:56:20.72 Intake @37.0psia, vacuum from 20.0 to 24.0psi @08:56:20.73 SS.reconfigure forSampling @08:56:20.77 SS.seek 25.00ml @08:56:22.37 Vacuum too high -- waiting up to 40s @08:56:22.86 Intake @36.7psia, vacuum from 20.0 to 24.0psi @08:56:22.87 SS.reconfigure forSampling @08:56:22.90 SS.seek 25.00ml @08:56:24.53 Vacuum too high -- waiting up to 40s @08:56:24.99 Intake @37.2psia, vacuum from 20.0 to 24.0psi @08:56:25.00 SS.reconfigure forSampling @08:56:25.04 SS.seek 25.00ml @08:56:26.66 Vacuum too high -- waiting up to 40s @08:56:27.17 Intake @37.9psia, vacuum from 20.0 to 24.0psi @08:56:27.18 SS.reconfigure forSampling @08:56:27.22 SS.seek 25.00ml @08:56:28.84 Vacuum too high -- waiting up to 40s @08:56:29.10 Intake @37.2psia, vacuum from 20.0 to 24.0psi @08:56:29.11 SS.reconfigure forSampling @08:56:29.14 SS.seek 25.00ml @08:56:30.31 Vacuum too high -- waiting up to 40s @08:56:30.57 Intake @36.7psia, vacuum from 20.0 to 24.0psi @08:56:30.58 SS.reconfigure forSampling @08:56:30.61 SS.seek 25.00ml @08:56:31.84 Vacuum too high -- waiting up to 40s @08:56:32.32 Intake @36.8psia, vacuum from 20.0 to 24.0psi @08:56:32.33 SS.reconfigure forSampling @08:56:32.36 SS.seek 25.00ml @08:56:33.84 Vacuum too high -- waiting up to 40s @08:56:34.10 Intake @36.6psia, vacuum from 20.0 to 24.0psi @08:56:34.11 SS.reconfigure forSampling @08:56:34.14 SS.seek 25.00ml @08:56:35.41 Vacuum too high -- waiting up to 40s @08:56:35.87 Intake @36.9psia, vacuum from 20.0 to 24.0psi @08:56:35.88 SS.reconfigure forSampling @08:56:35.92 SS.seek 25.00ml @08:56:37.56 Vacuum too high -- waiting up to 40s @08:56:38.03 Intake @36.9psia, vacuum from 20.0 to 24.0psi @08:56:38.04 SS.reconfigure forSampling @08:56:38.07 SS.seek 25.00ml @08:56:39.56 Vacuum too high -- waiting up to 40s @08:56:40.02 Intake @36.8psia, vacuum from 20.0 to 24.0psi @08:56:40.03 SS.reconfigure forSampling @08:56:40.07 SS.seek 25.00ml @08:56:41.53 Vacuum too high -- waiting up to 40s @08:56:41.99 Intake @37.1psia, vacuum from 20.0 to 24.0psi @08:56:42.00 SS.reconfigure forSampling @08:56:42.04 SS.seek 25.00ml @08:56:43.84 Vacuum too high -- waiting up to 40s @08:56:44.30 Intake @37.9psia, vacuum from 20.0 to 24.0psi @08:56:44.31 SS.reconfigure forSampling @08:56:44.35 SS.seek 25.00ml @08:56:45.81 Vacuum too high -- waiting up to 40s @08:56:46.06 Intake @37.5psia, vacuum from 20.0 to 24.0psi @08:56:46.07 SS.reconfigure forSampling @08:56:46.11 SS.seek 25.00ml @08:56:47.22 Vacuum too high -- waiting up to 40s @08:56:47.68 Intake @37.2psia, vacuum from 20.0 to 24.0psi @08:56:47.69 SS.reconfigure forSampling @08:56:47.73 SS.seek 25.00ml @08:56:49.25 Vacuum too high -- waiting up to 40s @08:56:49.72 Intake @37.1psia, vacuum from 20.0 to 24.0psi @08:56:50.21 SS.reconfigure forSampling @08:56:50.24 SS.seek 25.00ml @08:56:52.06 Vacuum too high -- waiting up to 40s @08:56:52.32 Intake @36.6psia, vacuum from 20.0 to 24.0psi @08:56:52.33 SS.reconfigure forSampling @08:56:52.37 SS.seek 25.00ml @08:56:53.50 Vacuum too high -- waiting up to 40s @08:56:53.98 Intake @36.5psia, vacuum from 20.0 to 24.0psi @08:56:53.99 SS.reconfigure forSampling @08:56:54.02 SS.seek 25.00ml @08:56:55.50 Vacuum too high -- waiting up to 40s @08:56:55.98 Intake @36.5psia, vacuum from 20.0 to 24.0psi @08:56:55.99 SS.reconfigure forSampling @08:56:56.02 SS.seek 25.00ml @08:56:57.05 Stroke completed -- waiting for pressure to equalize ... @08:56:58.28 Intake.select :close @08:56:58.41 SSV.dial :exhaust @08:56:59.20 Exhaust.select :open @08:56:59.29 SS.reconfigure SSexhale @08:56:59.33 SS.seek 0.00ml @08:57:07.93 Exhaust.select :close @08:57:08.02 SSV.dial CBV @08:57:08.04
CBV.dial SSV @08:57:08.84 Allowing 185s for 25.0ml of remaining 750.0ml @08:57:08.85 Intake.select :open @08:57:10.98 Intake @36.7psia, vacuum from 20.0 to 24.0psi @08:57:10.99 SS.reconfigure forSampling @08:57:11.03 SS.seek 25.00ml @08:57:13.53 Vacuum too high -- waiting up to 40s @08:57:14.01 Intake @36.4psia, vacuum from 20.0 to 24.0psi @08:57:14.02 SS.reconfigure forSampling @08:57:14.06 SS.seek 25.00ml @08:57:15.72 Vacuum too high -- waiting up to 40s @08:57:16.19 Intake @36.9psia, vacuum from 20.0 to 24.0psi @08:57:16.20 SS.reconfigure forSampling @08:57:16.23 SS.seek 25.00ml @08:57:17.87 Vacuum too high -- waiting up to 40s @08:57:18.34 Intake @36.9psia, vacuum from 20.0 to 24.0psi @08:57:18.35 SS.reconfigure forSampling @08:57:18.38 SS.seek 25.00ml @08:57:20.12 Vacuum too high -- waiting up to 40s @08:57:20.59 Intake @37.2psia, vacuum from 20.0 to 24.0psi @08:57:20.60 SS.reconfigure forSampling @08:57:20.63 SS.seek 25.00ml @08:57:22.28 Vacuum too high -- waiting up to 40s @08:57:22.76 Intake @37.2psia, vacuum from 20.0 to 24.0psi @08:57:22.77 SS.reconfigure forSampling @08:57:22.80 SS.seek 25.00ml @08:57:24.43 Vacuum too high -- waiting up to 40s @08:57:24.91 Intake @37.3psia, vacuum from 20.0 to 24.0psi @08:57:24.92 SS.reconfigure forSampling @08:57:24.96 SS.seek 25.00ml @08:57:26.62 Vacuum too high -- waiting up to 40s @08:57:26.87 Intake @36.6psia, vacuum from 20.0 to 24.0psi @08:57:26.88 SS.reconfigure forSampling @08:57:26.92 SS.seek 25.00ml @08:57:28.28 Vacuum too high -- waiting up to 40s @08:57:28.74 Intake @36.9psia, vacuum from 20.0 to 24.0psi @08:57:28.75 SS.reconfigure forSampling @08:57:28.79 SS.seek 25.00ml @08:57:30.46 Vacuum too high -- waiting up to 40s @08:57:30.92 Intake @36.6psia, vacuum from 20.0 to 24.0psi @08:57:30.93 SS.reconfigure forSampling @08:57:30.97 SS.seek 25.00ml @08:57:32.68 Vacuum too high -- waiting up to 40s @08:57:33.14 Intake @36.8psia, vacuum from 20.0 to 24.0psi @08:57:33.15 SS.reconfigure forSampling @08:57:33.19 SS.seek 25.00ml @08:57:34.78 Vacuum too high -- waiting up to 40s @08:57:35.36 Intake @36.9psia, vacuum from 20.0 to 24.0psi @08:57:35.37 SS.reconfigure forSampling @08:57:35.40 SS.seek 25.00ml @08:57:37.12 Vacuum too high -- waiting up to 40s @08:57:37.58 Intake @36.5psia, vacuum from 20.0 to 24.0psi @08:57:37.59 SS.reconfigure forSampling @08:57:37.63 SS.seek 25.00ml @08:57:39.25 Vacuum too high -- waiting up to 40s @08:57:39.72 Intake @37.2psia, vacuum from 20.0 to 24.0psi @08:57:39.73 SS.reconfigure forSampling @08:57:39.77 SS.seek 25.00ml @08:57:41.18 Vacuum too high -- waiting up to 40s @08:57:41.43 Intake @36.4psia, vacuum from 20.0 to 24.0psi @08:57:41.44 SS.reconfigure forSampling @08:57:41.48 SS.seek 25.00ml @08:57:42.68 Vacuum too high -- waiting up to 40s @08:57:43.66 SS.seek 25.00ml @08:57:45.65 Vacuum too high -- waiting up to 40s @08:57:46.13 Intake @36.9psia, vacuum from 20.0 to 24.0psi @08:57:46.14 SS.reconfigure forSampling @08:57:46.17 SS.seek 25.00ml @08:57:47.68 Vacuum too high -- waiting up to 40s @08:57:48.16 Intake @36.7psia, vacuum from 20.0 to 24.0psi @08:57:48.17 SS.reconfigure forSampling @08:57:48.20 SS.seek 25.00ml @08:57:49.81 Vacuum too high -- waiting up to 40s @08:57:50.29 Intake @37.2psia, vacuum from 20.0 to 24.0psi @08:57:50.30 SS.reconfigure forSampling @08:57:50.33 SS.seek 25.00ml @08:57:51.84 Vacuum too high -- waiting up to 40s @08:57:52.32 Intake @36.6psia, vacuum from 20.0 to 24.0psi @08:57:52.33 SS.reconfigure forSampling @08:57:52.36 SS.seek 25.00ml @08:57:53.84 Vacuum too high -- waiting up to 40s @08:57:54.31 Intake @36.3psia, vacuum from 20.0 to 24.0psi @08:57:54.32 SS.reconfigure forSampling @08:57:54.35 SS.seek 25.00ml @08:57:55.68 Vacuum too high -- waiting up to 40s @08:57:56.16 Intake @36.3psia, vacuum from 20.0 to 24.0psi @08:57:56.17 SS.reconfigure forSampling @08:57:56.20 SS.seek 25.00ml @08:57:57.68 Vacuum too high -- waiting up to 40s @08:57:58.15 Intake @36.6psia, vacuum from 20.0 to 24.0psi @08:57:58.16 SS.reconfigure forSampling @08:57:58.20 SS.seek 25.00ml @08:57:59.62 Vacuum too high -- waiting up to 40s @08:58:00.09 Intake @37.3psia, vacuum from 20.0 to 24.0psi @08:58:00.10 SS.reconfigure forSampling @08:58:00.14 SS.seek 25.00ml @08:58:01.46 Vacuum too high -- waiting up to 40s @08:58:01.94 Intake @36.6psia, vacuum from 20.0 to 24.0psi @08:58:01.95 SS.reconfigure forSampling @08:58:01.98 SS.seek 25.00ml @08:58:03.37 Vacuum too high -- waiting up to 40s @08:58:03.83 Intake @36.3psia, vacuum from 20.0 to 24.0psi @08:58:03.84 SS.reconfigure forSampling @08:58:03.88 SS.seek 25.00ml @08:58:05.21 Vacuum too high -- waiting up to 40s @08:58:05.68 Intake @36.2psia, vacuum from 20.0 to 24.0psi @08:58:05.69 SS.reconfigure forSampling @08:58:05.72 SS.seek 25.00ml @08:58:07.03 Vacuum too high -- waiting up to 40s @08:58:07.49 Intake @35.9psia, vacuum from 20.0 to 24.0psi @08:58:07.50 SS.reconfigure forSampling @08:58:07.54 SS.seek 25.00ml @08:58:08.96 Vacuum too high -- waiting up to 40s @08:58:09.42 Intake @36.6psia, vacuum from 20.0 to 24.0psi @08:58:09.43 SS.reconfigure forSampling @08:58:09.47 SS.seek 25.00ml @08:58:10.90 Vacuum too high -- waiting up to 40s @08:58:11.36 Intake @36.9psia, vacuum from 20.0 to 24.0psi @08:58:11.37 SS.reconfigure forSampling @08:58:11.41 SS.seek 25.00ml @08:58:12.68 Vacuum too high -- waiting up to 40s @08:58:12.93 Intake @36.3psia, vacuum from 20.0 to 24.0psi @08:58:12.94 SS.reconfigure forSampling @08:58:12.98 SS.seek 25.00ml @08:58:13.99 Vacuum too high -- waiting up to 40s @08:58:14.46 Intake @36.2psia, vacuum from 20.0 to 24.0psi @08:58:14.47 SS.reconfigure forSampling @08:58:14.50 SS.seek 25.00ml @08:58:15.81 Vacuum too high -- waiting up to 40s @08:58:16.27 Intake @36.4psia, vacuum from 20.0 to 24.0psi @08:58:16.28 SS.reconfigure forSampling @08:58:16.32 SS.seek 25.00ml @08:58:17.49 Vacuum too high -- waiting up to 40s @08:58:17.95 Intake @36.5psia, vacuum from 20.0 to 24.0psi @08:58:17.96 SS.reconfigure forSampling @08:58:18.00 SS.seek 25.00ml @08:58:19.31 Vacuum too high -- waiting up to 40s @08:58:19.77 Intake @36.7psia, vacuum from 20.0 to 24.0psi @08:58:19.79 SS.reconfigure forSampling @08:58:19.82 SS.seek 25.00ml @08:58:21.15 Vacuum too high -- waiting up to 40s @08:58:21.61 Intake @36.4psia, vacuum from 20.0 to 24.0psi @08:58:21.62 SS.reconfigure forSampling @08:58:21.66 SS.seek 25.00ml @08:58:24.02 Vacuum too high -- waiting up to 40s @08:58:24.50 Intake @37.2psia, vacuum from 20.0 to 24.0psi @08:58:24.51 SS.reconfigure forSampling @08:58:24.54 SS.seek 25.00ml @08:58:25.71 Vacuum too high -- waiting up to 40s @08:58:26.18 Intake @36.6psia, vacuum from 20.0 to 24.0psi @08:58:26.19 SS.reconfigure forSampling @08:58:26.22 SS.seek 25.00ml @08:58:27.49 Vacuum too high -- waiting up to 40s @08:58:27.97 Intake @36.4psia, vacuum from 20.0 to 24.0psi @08:58:27.98 SS.reconfigure forSampling @08:58:28.01 SS.seek 25.00ml @08:58:29.24 Vacuum too high -- waiting up to 40s @08:58:29.50 Intake @36.3psia, vacuum from 20.0 to 24.0psi @08:58:29.51 SS.reconfigure forSampling @08:58:29.55 SS.seek 25.00ml @08:58:30.43 Vacuum too high -- waiting up to 40s @08:58:30.90 Intake @36.2psia, vacuum from 20.0 to 24.0psi @08:58:30.91 SS.reconfigure forSampling @08:58:30.94 SS.seek 25.00ml @08:58:32.12 Vacuum too high -- waiting up to 40s @08:58:32.60 Intake @36.1psia, vacuum from 20.0 to 24.0psi @08:58:32.61 SS.reconfigure forSampling @08:58:32.65 SS.seek 25.00ml @08:58:33.90 Vacuum too high -- waiting up to 40s @08:58:34.38 SS.seek 25.00ml @08:58:35.43 Vacuum too high -- waiting up to 40s @08:58:35.90 Intake @35.9psia, vacuum from 20.0 to 24.0psi @08:58:35.91 SS.reconfigure forSampling @08:58:35.95 SS.seek 25.00ml @08:58:37.24 Vacuum too high -- waiting up to 40s @08:58:37.71 Intake @36.6psia, vacuum from 20.0 to 24.0psi @08:58:37.72 SS.reconfigure forSampling @08:58:37.75 SS.seek 25.00ml @08:58:38.93 Vacuum too high -- waiting up to 40s @08:58:39.40 Intake @37.4psia, vacuum from 20.0 to 24.0psi @08:58:39.41 SS.reconfigure forSampling @08:58:39.44 SS.seek 25.00ml @08:58:40.59 Vacuum too high -- waiting up to 40s @08:58:41.06 Intake @36.9psia, vacuum from 20.0 to 24.0psi @08:58:41.07 SS.reconfigure forSampling @08:58:41.11 SS.seek 25.00ml @08:58:42.37 Vacuum too high -- waiting up to 40s @08:58:42.85 Intake @36.5psia, vacuum from 20.0 to 24.0psi @08:58:42.86 SS.reconfigure forSampling @08:58:42.89 SS.seek 25.00ml @08:58:44.12 Vacuum too high -- waiting up to 40s @08:58:44.58 Intake @36.5psia, vacuum from 20.0 to 24.0psi @08:58:44.59 SS.reconfigure forSampling @08:58:44.64 SS.seek 25.00ml @08:58:45.84 Vacuum too high -- waiting up to 40s @08:58:46.30 Intake @36.3psia, vacuum from 20.0 to 24.0psi @08:58:46.31 SS.reconfigure forSampling @08:58:46.35 SS.seek 25.00ml @08:58:47.49 Vacuum too high -- waiting up to 40s @08:58:47.95 Intake @36.3psia, vacuum from 20.0 to 24.0psi @08:58:47.96 SS.reconfigure forSampling @08:58:48.00 SS.seek 25.00ml @08:58:49.12 Vacuum too high -- waiting up to 40s @08:58:49.59 SS.seek 25.00ml @08:58:50.68 Vacuum too high -- waiting up to 40s @08:58:51.14 Intake @36.0psia, vacuum from 20.0 to 24.0psi @08:58:51.15 SS.reconfigure forSampling @08:58:51.19 SS.seek 25.00ml @08:58:52.40 Vacuum too high -- waiting up to 40s @08:58:52.88 Intake @36.0psia, vacuum from 20.0 to 24.0psi @08:58:52.89 SS.reconfigure forSampling @08:58:52.92 SS.seek 25.00ml @08:58:54.09 Vacuum too high -- waiting up to 40s @08:58:54.55 Intake @36.1psia, vacuum from 20.0 to 24.0psi @08:58:54.56 SS.reconfigure forSampling @08:58:54.60 SS.seek 25.00ml @08:58:55.65 Vacuum too high -- waiting up to 40s @08:58:56.11 Intake @36.4psia, vacuum from 20.0 to 24.0psi @08:58:56.12 SS.reconfigure forSampling @08:58:56.16 SS.seek 25.00ml @08:58:57.27 Vacuum too high -- waiting up to 40s @08:58:58.28 Intake @37.1psia, vacuum from 20.0 to 24.0psi @08:58:58.29 SS.reconfigure forSampling @08:58:58.32 SS.seek 25.00ml @08:58:59.99 Vacuum too high -- waiting up to 40s @08:59:00.46 Intake @36.6psia, vacuum from 20.0 to 24.0psi @08:59:00.47 SS.reconfigure forSampling @08:59:00.50 SS.seek 25.00ml @08:59:01.68 Vacuum too high -- waiting up to 40s @08:59:02.16 Intake @36.6psia, vacuum from 20.0 to 24.0psi @08:59:02.17 SS.reconfigure forSampling @08:59:02.20 SS.seek 25.00ml @08:59:03.30 Vacuum too high -- waiting up to 40s @08:59:03.77 Intake @36.7psia, vacuum from 20.0 to 24.0psi @08:59:03.78 SS.reconfigure forSampling @08:59:03.81 SS.seek 25.00ml @08:59:04.90 Vacuum too high -- waiting up to 40s @08:59:05.37 Intake @36.6psia, vacuum from 20.0 to 24.0psi @08:59:05.38 SS.reconfigure forSampling @08:59:05.41 SS.seek 25.00ml @08:59:06.52 Vacuum too high -- waiting up to 40s @08:59:07.00 Intake @36.7psia, vacuum from 20.0 to 24.0psi @08:59:07.01 SS.reconfigure forSampling @08:59:07.04 SS.seek 25.00ml @08:59:08.24 Vacuum too high -- waiting up to 40s @08:59:08.71 Intake @36.8psia, vacuum from 20.0 to 24.0psi @08:59:08.72 SS.reconfigure forSampling @08:59:08.75 SS.seek 25.00ml @08:59:09.77 Vacuum too high -- waiting up to 40s @08:59:10.24 Intake @36.6psia, vacuum from 20.0 to 24.0psi @08:59:10.25 SS.reconfigure forSampling @08:59:10.29 SS.seek 25.00ml @08:59:11.43 Vacuum too high -- waiting up to 40s @08:59:11.90 Intake @36.6psia, vacuum from 20.0 to 24.0psi @08:59:11.91 SS.reconfigure forSampling @08:59:11.95 SS.seek 25.00ml @08:59:13.08 Vacuum too high -- waiting up to 40s @08:59:13.55 Intake @37.8psia, vacuum from 20.0 to 24.0psi @08:59:13.56 SS.reconfigure forSampling @08:59:13.60 SS.seek 25.00ml @08:59:14.65 Vacuum too high -- waiting up to 40s @08:59:15.12 Intake @37.0psia, vacuum from 20.0 to 24.0psi @08:59:15.13 SS.reconfigure forSampling @08:59:15.17 SS.seek 25.00ml @08:59:16.27 Vacuum too high -- waiting up to 40s @08:59:16.74 Intake @36.9psia, vacuum from 20.0 to 24.0psi @08:59:16.75 SS.reconfigure forSampling @08:59:16.78 SS.seek 25.00ml @08:59:17.87 Vacuum too high -- waiting up to 40s @08:59:18.34 SS.seek 25.00ml @08:59:19.43 Vacuum too high -- waiting up to 40s @08:59:19.90 Intake @36.8psia, vacuum from 20.0 to 24.0psi @08:59:19.91 SS.reconfigure forSampling @08:59:19.94 SS.seek 25.00ml @08:59:20.99 Vacuum too high -- waiting up to 40s @08:59:21.45 Intake @36.7psia, vacuum from 20.0 to 24.0psi @08:59:21.46 SS.reconfigure forSampling @08:59:21.49 SS.seek 25.00ml @08:59:22.52 Vacuum too high -- waiting up to 40s @08:59:23.00 SS.seek 25.00ml @08:59:23.96 Vacuum too high -- waiting up to 40s @08:59:24.42 Intake @36.6psia, vacuum from 20.0 to 24.0psi @08:59:24.43 SS.reconfigure forSampling @08:59:24.47 SS.seek 25.00ml @08:59:25.52 Vacuum too high -- waiting up to 40s @08:59:25.99 Intake @36.7psia, vacuum from 20.0 to 24.0psi @08:59:26.00 SS.reconfigure forSampling @08:59:26.03 SS.seek 25.00ml @08:59:27.11 Vacuum too high -- waiting up to 40s @08:59:27.58 Intake @37.6psia, vacuum from 20.0 to 24.0psi @08:59:27.59 SS.reconfigure forSampling @08:59:27.62 SS.seek 25.00ml @08:59:28.61 Vacuum too high -- waiting up to 40s @08:59:29.08 Intake @37.3psia, vacuum from 20.0 to 24.0psi @08:59:29.09 SS.reconfigure forSampling @08:59:29.12 SS.seek 25.00ml @08:59:30.39 Vacuum too high -- waiting up to 40s @08:59:30.65 Intake @36.9psia, vacuum from 20.0 to 24.0psi @08:59:31.21 SS.reconfigure forSampling @08:59:31.24 SS.seek 25.00ml @08:59:32.90 Vacuum too high -- waiting up to 40s @08:59:33.38 Intake @36.8psia, vacuum from 20.0 to 24.0psi @08:59:33.39 SS.reconfigure forSampling @08:59:33.42 SS.seek 25.00ml @08:59:34.52 Vacuum too high -- waiting up to 40s @08:59:34.99 Intake @36.9psia, vacuum from 20.0 to 24.0psi @08:59:35.00 SS.reconfigure forSampling @08:59:35.04 SS.seek 25.00ml @08:59:36.11 Vacuum too high -- waiting up to 40s @08:59:36.59 Intake @36.6psia, vacuum from 20.0 to 24.0psi @08:59:36.60 SS.reconfigure forSampling @08:59:36.63 SS.seek 25.00ml @08:59:37.68 Vacuum too high -- waiting up to 40s @08:59:38.15 Intake @36.6psia, vacuum from 20.0 to 24.0psi @08:59:38.16 SS.reconfigure forSampling @08:59:38.20 SS.seek 25.00ml @08:59:39.21 Vacuum too high -- waiting up to 40s @08:59:39.68 Intake @36.3psia, vacuum from 20.0 to 24.0psi @08:59:39.69 SS.reconfigure forSampling @08:59:39.73 SS.seek 25.00ml @08:59:40.80 Vacuum too high -- waiting up to 40s @08:59:41.27 Intake @36.5psia, vacuum from 20.0 to 24.0psi @08:59:41.28 SS.reconfigure forSampling @08:59:41.32 SS.seek 25.00ml @08:59:42.43 Vacuum too high -- waiting up to 40s @08:59:42.90 Intake @37.7psia, vacuum from 20.0 to 24.0psi @08:59:42.91 SS.reconfigure forSampling @08:59:42.94 SS.seek 25.00ml @08:59:43.96 Vacuum too high -- waiting up to 40s @08:59:44.43 Intake @37.4psia, vacuum from 20.0 to 24.0psi @08:59:44.44 SS.reconfigure forSampling @08:59:44.47 SS.seek 25.00ml @08:59:45.52 Vacuum too high -- waiting up to 40s @08:59:46.00 Intake @37.0psia, vacuum from 20.0 to 24.0psi @08:59:46.01 SS.reconfigure forSampling @08:59:46.05 SS.seek 25.00ml @08:59:47.11 Vacuum too high -- waiting up to 40s @08:59:47.59 Intake @36.5psia, vacuum from 20.0 to 24.0psi @08:59:47.60 SS.reconfigure forSampling @08:59:47.63 SS.seek 25.00ml @08:59:48.74 Vacuum too high -- waiting up to 40s @08:59:49.22 Intake @36.8psia, vacuum from 20.0 to 24.0psi @08:59:49.23 SS.reconfigure forSampling @08:59:49.26 SS.seek 25.00ml @08:59:50.24 Vacuum too high -- waiting up to 40s @08:59:50.71 Intake @36.8psia, vacuum from 20.0 to 24.0psi @08:59:50.72 SS.reconfigure forSampling @08:59:50.75 SS.seek 25.00ml @08:59:51.74 Vacuum too high -- waiting up to 40s @08:59:52.21 Intake @36.7psia, vacuum from 20.0 to 24.0psi @08:59:52.22 SS.reconfigure forSampling @08:59:52.25 SS.seek 25.00ml @08:59:53.33 Vacuum too high -- waiting up to 40s @08:59:53.81 Intake @36.6psia, vacuum from 20.0 to 24.0psi @08:59:53.82 SS.reconfigure forSampling @08:59:53.85 SS.seek 25.00ml @08:59:54.86 Vacuum too high -- waiting up to 40s @08:59:55.33 Intake @36.6psia, vacuum from 20.0 to 24.0psi @08:59:55.34 SS.reconfigure forSampling @08:59:55.37 SS.seek 25.00ml @08:59:56.36 Vacuum too high -- waiting up to 40s @08:59:56.83 Intake @36.5psia, vacuum from 20.0 to 24.0psi @08:59:56.84 SS.reconfigure forSampling @08:59:56.87 SS.seek 25.00ml @08:59:57.86 Vacuum too high -- waiting up to 40s @08:59:58.33 SS.seek 25.00ml @08:59:59.30 Vacuum too high -- waiting up to 40s @08:59:59.92 Intake @36.3psia, vacuum from 20.0 to 24.0psi @08:59:59.93 SS.reconfigure forSampling @08:59:59.96 SS.seek 25.00ml @09:00:01.21 Vacuum too high -- waiting up to 40s @09:00:01.67 Intake @37.1psia, vacuum from 20.0 to 24.0psi @09:00:01.68 SS.reconfigure forSampling @09:00:01.72 SS.seek 25.00ml @09:00:02.71 Vacuum too high -- waiting up to 40s @09:00:03.17 Intake @37.7psia, vacuum from 20.0 to 24.0psi @09:00:03.73 SS.reconfigure forSampling @09:00:03.76 SS.seek 25.00ml @09:00:05.21 Vacuum too high -- waiting up to 40s @09:00:05.69 Intake @36.9psia, vacuum from 20.0 to 24.0psi @09:00:05.70 SS.reconfigure forSampling @09:00:05.73 SS.seek 25.00ml @09:00:06.77 Vacuum too high -- waiting up to 40s @09:00:07.24 Intake @37.0psia, vacuum from 20.0 to 24.0psi @09:00:07.25 SS.reconfigure forSampling @09:00:07.28 SS.seek 25.00ml @09:00:08.30 Vacuum too high -- waiting up to 40s @09:00:08.77 Intake @36.5psia, vacuum from 20.0 to 24.0psi @09:00:08.78 SS.reconfigure forSampling @09:00:08.81 SS.seek 25.00ml @09:00:09.83 Vacuum too high -- waiting up to 40s @09:00:10.30 Intake @36.9psia, vacuum from 20.0 to 24.0psi @09:00:10.31 SS.reconfigure forSampling @09:00:10.34 SS.seek 25.00ml @09:00:11.36 Vacuum too high -- waiting up to 40s @09:00:11.84 Intake @36.9psia, vacuum from 20.0 to 24.0psi @09:00:11.85 SS.reconfigure forSampling @09:00:11.88 SS.seek 25.00ml @09:00:12.86 Vacuum too high -- waiting up to 40s @09:00:13.34 Intake @37.0psia, vacuum from 20.0 to 24.0psi @09:00:13.35 SS.reconfigure forSampling @09:00:13.39 SS.seek 25.00ml @09:00:13.86 SS.reconfigure SSconfig @09:00:13.89 SS.hold @09:00:14.02 Intake.select :close @09:00:14.16 ShallowSampler::Clogged in MAIN -- progress too slow after 274.1ml SSV.dial :exhaust @09:00:14.95 Exhaust.select :open @09:00:15.06 SS.reconfigure SSexhale @09:00:15.10 SS.seek 0.00ml @09:00:23.62 Exhaust.select :close @09:00:23.72 Sampled 274.1ml @09:00:23.73 collectSample: Complete Evacuating Puck (bubblePt=55.0psi, delay=15.0s) @09:00:23.74 Intake.select :close @09:00:23.80 CSV.dial :waste @09:00:23.83 CS.seek 0.000ml @09:00:23.92 SSV.dial CBV,avoiding => exhaust @09:00:23.95
CBV.dial SSV @09:00:23.98 CSV.dial :air @09:00:24.82 CS.seek 10.000ml @09:00:25.93
CTV.dial :air @09:00:26.72 delay 15 seconds @09:00:26.73 Sleepy.power :sampler,:OFF @09:00:41.72
SSV.dial :spit,avoiding => exhaust @09:00:43.04 CTV.dial CSV,avoiding => intake @09:00:43.06
SS.seek 0.00ml @09:00:43.15 SSV.dial CBV,avoiding => exhaust @09:00:49.47 CBV.dial between CSV and waste2b @09:00:50.41 CTV.dial CSV,avoiding => air @09:00:50.42
CSV.dial CTV @09:00:50.86 CS.reconfigure CSpressurize @09:00:50.89 CS.seek 0.000ml @09:01:15.68 BubblePoint: Intake @69.9psia, Outlet @15.2psia @09:01:15.69 SS.reconfigure SSconfig @09:01:15.72 SS.seek 10.00ml @09:01:21.79 delay 15 seconds @09:01:36.89 Evacuated: Intake @68.4psia, Outlet @-0.3psia SSV.dial :spit,avoiding => exhaust @09:01:38.22 SS.reconfigure SSexhale @09:01:38.25 SS.seek 0.00ml @09:01:43.35 SS.reconfigure SSfast @09:01:43.38 SS.seek 5.00ml @09:01:46.32 SSV.dial CBV,avoiding => exhaust @09:01:46.34
CBV.dial SSV @09:01:47.69 CTV.dial :air,avoiding => intake @09:01:48.99 CSV.dial CTV @09:01:49.01
SS.reconfigure SSconfig @09:01:49.05 SS.seek 10.00ml @09:01:50.01 CSV.dial :air @09:01:50.53 CS.reconfigure CSfast @09:01:50.56 CS.seek 0.000ml @09:01:52.26
SSV.dial :spit,avoiding => exhaust @09:01:52.35 CS.coast @09:01:53.58
SS.reconfigure SSexhale @09:01:53.61 SS.seek 0.00ml @09:01:58.37 SS.reconfigure SSfast @09:01:58.40 SS.seek 5.00ml @09:02:01.24 SSV.dial CBV,avoiding => exhaust @09:02:01.26
CBV.dial SSV @09:02:03.57 SSV.dial :spit,avoiding => exhaust @09:02:04.89 SS.reconfigure SSconfig @09:02:04.92 SS.seek 0.00ml @09:02:08.12 SS.coast @09:02:08.15 Evacuating Puck (bubblePt=55.0psi, delay=15.0s) Intake.select :close @09:02:08.21 CSV.dial :waste @09:02:08.30
SSV.dial :exhaust @09:02:08.95 CS.seek 0.000ml @09:02:09.05
Exhaust.select :open @09:02:09.16 SS.reconfigure SSexhale @09:02:09.20 CSV.dial :air @09:02:09.73
SS.seek 0.00ml @09:02:10.19 Exhaust.select :close @09:02:10.28 SSV.dial CBV,avoiding => exhaust @09:02:10.30
CBV.dial SSV @09:02:10.53 CS.seek 10.000ml @09:02:12.17
CTV.dial :air @09:02:12.18 delay 15 seconds @09:02:27.18 SSV.dial :spit,avoiding => exhaust @09:02:28.50 CTV.dial CSV,avoiding => intake @09:02:28.52
SS.seek 0.00ml @09:02:28.62 SSV.dial CBV,avoiding => exhaust @09:02:34.49 CBV.dial between CSV and waste2b @09:02:35.43 CSV.dial between air and CTV @09:02:35.75 CS.seek 7.417ml @09:02:42.61 CTV.dial CSV,avoiding => air @09:02:42.63
CSV.dial CTV @09:02:42.92 CS.reconfigure CSpressurize @09:02:42.95 CS.seek 0.000ml @09:03:02.25 CSV.dial :air @09:03:02.78 CS.reconfigure CSfast @09:03:02.81 CS.seek 10.000ml @09:03:26.52 CSV.dial between air and CTV @09:03:26.91 CS.seek 2.347ml @09:03:45.43 CTV.dial CSV,avoiding => air @09:03:45.44
CSV.dial CTV @09:03:45.74 CS.reconfigure CSpressurize @09:03:45.77 CS.seek 0.000ml @09:03:48.69 BubblePoint: Intake @72.9psia, Outlet @17.4psia @09:03:48.70 SS.reconfigure SSconfig @09:03:48.73 SS.seek 10.00ml @09:03:54.54 delay 15 seconds @09:04:09.64 Evacuated: Intake @72.0psia, Outlet @-0.4psia @09:04:09.65 SSV.dial :spit,avoiding => exhaust @09:04:10.96 SS.reconfigure SSexhale @09:04:10.99 SS.seek 0.00ml @09:04:15.93 SS.reconfigure SSfast @09:04:15.97 SS.seek 5.00ml @09:04:18.90 SSV.dial CBV,avoiding => exhaust @09:04:18.92
CBV.dial SSV @09:04:20.26 CTV.dial :air,avoiding => intake @09:04:21.56 CSV.dial CTV @09:04:21.58
SS.reconfigure SSconfig @09:04:21.62 SS.seek 10.00ml @09:04:22.58 CSV.dial :air @09:04:23.30 CS.reconfigure CSfast @09:04:23.33 CS.seek 0.000ml @09:04:24.87
SSV.dial :spit,avoiding => exhaust @09:04:26.19 SS.reconfigure SSexhale @09:04:26.22 SS.seek 0.00ml @09:04:27.38 CS.coast @09:04:31.29
SS.reconfigure SSfast @09:04:31.33 SS.seek 5.00ml @09:04:34.21 SSV.dial CBV,avoiding => exhaust @09:04:34.23
CBV.dial SSV @09:04:36.53 SSV.dial :spit,avoiding => exhaust @09:04:37.85 SS.reconfigure SSconfig @09:04:37.88 SS.seek 0.00ml @09:04:41.09 SS.coast @09:04:41.12 Lysing: Adding 1.5ml lysis CBV.dial between CSV and waste1,avoiding => DBV @09:04:42.61 CTV.dial CSV @09:04:43.87 CSV.dial CSR @09:04:44.57 CSR.select :lysis @09:04:44.67 CS.reconfigure CSconfig @09:04:44.71 CS.seek 1.000ml @09:04:48.01 delay 5 seconds @09:04:53.02 CSR.select :air @09:04:53.16 CS.seek 6.000ml @09:05:06.63 delay 2 seconds @09:05:08.64 CSV.dial :waste1 @09:05:09.91 CS.reconfigure CSfast @09:05:09.94 CS.seek empty @09:05:24.44 CSV.dial CSR @09:05:25.71 CSR.select :lysis @09:05:25.81 CS.reconfigure CSconfig @09:05:25.85 CS.seek 1.500ml @09:05:30.54 delay 5 seconds @09:05:35.56 CSR.select :air @09:05:35.70 CS.seek 4.500ml @09:05:44.07 delay 2 seconds @09:05:46.08 CSV.dial CTV @09:05:46.59 CS.seek 1.275ml @09:05:55.61 Heating sh1 puck from tube 2 in Collection Clamp to 85.0C for 5:00 @09:05:55.62 CH.seek 85.0C,8:00 @09:08:55.08 CH.hold 5:00,85.0C @09:13:55.18 Collection Puck Heater at 84.9C after lysing @09:13:55.19 CS.seek 0.000ml @09:13:59.27 CTV.dial between spare and air,avoiding => intake @09:14:00.27 CSV.dial :air @09:14:00.71 CS.seek 5.000ml @09:14:14.17 CSV.dial CTV @09:14:14.67 delay 2 seconds @09:14:16.68 CSV.dial :air @09:14:17.19 delay 2 seconds @09:14:19.20 CSV.dial CTV @09:14:19.70 delay 2 seconds @09:14:21.71 CSV.dial :air @09:14:22.20 delay 2 seconds @09:14:24.21 CS.seek 0.000ml @09:14:37.70 Recovering Lysate into Collection syringe @09:14:37.71 CSV.dial CBV @09:14:38.70 CBV.dial CSV @09:14:39.05 CS.seek 5.000ml @09:14:52.58 delay 5 seconds @09:14:57.59 CTV.dial :air @09:14:58.06 CS.seek 7.001ml @09:15:04.51 delay 5 seconds @09:15:09.52 CSV.dial :air2 @09:15:09.94 CS.seek 10.000ml @09:15:18.39 CSV.dial between air and CTV,avoiding => CBV @09:15:19.52 Lysing Complete @09:15:19.57 Storing sh1 puck in Collection Clamp to tube 1 @09:15:19.70 SC.reconfigure SCtight @09:15:19.73 SC.seek 1 @09:15:21.98 SC.reconfigure SCloose @09:15:22.16 Elbow.reconfigure TightElbow @09:15:22.19 Elbow.seek 1 @09:15:23.86 Forearm.reconfigure TightForearm @09:15:23.89 Forearm.seek 1 + 800 counts @09:15:24.96 SE.reconfigure SEpick @09:15:25.01 SE.seek beamBreak @09:15:27.74 SE.configure SEconfig @09:15:27.86 Elbow.reconfigure LimpElbow @09:15:27.89 Forearm.reconfigure LooseForearm @09:15:27.93 Forearm.seek 1 @09:15:28.13 Elbow.hold @09:15:28.18 Elbow.reconfigure TightElbow @09:15:28.26 SE.seek 2.04 Pucks below Top Plate @09:15:28.55 Forearm.coast @09:15:28.58 Forearm.seek 1 + 331 counts @09:15:28.81 Forearm.reconfigure TightForearm @09:15:28.84 Forearm.seek 1 + 800 counts @09:15:29.07 SE.seek 3.04 Pucks below Top Plate @09:15:29.57 Forearm.reconfigure LooseForearm @09:15:29.61 Forearm.seek retract @09:15:30.53 Elbow.seek CC @09:15:32.02 Elbow.reconfigure LooseElbow @09:15:32.11 Forearm.seek CC @09:15:33.08 CC.seek sealed @09:15:34.01 Hand.grip @09:15:34.75 CC.seek open @09:15:38.08 Forearm.seek retract @09:15:39.05 Elbow.reconfigure TightElbow @09:15:39.09 Elbow.seek 1 @09:15:40.66 Elbow.reconfigure LimpElbow @09:15:40.70 Forearm.seek 1 @09:15:41.71 Elbow.hold @09:15:41.76 Elbow.reconfigure TightElbow @09:15:41.80 Hand.release @09:15:42.58 Forearm.coast @09:15:42.61 Forearm.seek 1 + 318 counts @09:15:42.83 Forearm.reconfigure TightForearm @09:15:42.93 Forearm.seek 1 + 800 counts @09:15:43.28 SE.seek down @09:15:44.39
Forearm.reconfigure LooseForearm @09:15:44.43 Forearm.seek retract @09:15:45.69 SE.coast @09:15:45.72
SC.coast @09:15:45.74 Tranferring lysate to PRV @09:15:45.75 CSV.dial :air @09:15:46.05 CS.seek 10.000ml @09:15:46.10 CSV.dial between air and CTV @09:15:46.49 AIV.dial :bypass @09:15:46.55 Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @09:15:46.70 Elbow.seek FlushPuck::Garage @09:15:48.46 Elbow.reconfigure LooseElbow @09:15:48.54 Forearm.seek garage @09:15:49.57 Hand.grip @09:15:50.34 Forearm.seek retract @09:15:51.34 Elbow.reconfigure TightElbow @09:15:51.38 Elbow.seek CC @09:15:54.17 Elbow.reconfigure LooseElbow @09:15:54.25 Forearm.reconfigure TightForearm @09:15:54.28 Forearm.seek CC @09:15:55.38 CC.seek closed @09:15:59.22 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 2.02 @09:15:59.28 Hand.release @09:16:00.04 Forearm.reconfigure LooseForearm @09:16:00.07 Forearm.seek retract @09:16:01.02 AWV.select :air @09:16:01.03 AIV.dial :bypass @09:16:01.04 PSV.dial PSR @09:16:02.45 PSR.select :flush @09:16:02.55 PS.seek 1.500ml @09:16:18.59 delay 5 seconds @09:16:23.60 PSV.dial :waste1 @09:16:24.03 PS.seek 0.000ml @09:16:40.12 PSV.dial PSR @09:16:40.56 PSR.select :air @09:16:40.66 PS.seek 2.500ml @09:17:06.94 CBV.dial PRV @09:17:06.95 PRV.dial CBV @09:17:07.73
PSV.dial :mixloop @09:17:08.74 CTV.dial :air,avoiding => intake @09:17:08.75 PTV.dial :PRVmixing @09:17:08.83 PS.seek 0.000ml @09:17:35.12 PSV.dial :air @09:17:35.56 PS.seek 2.500ml @09:18:01.86 PSV.dial :mixloop @09:18:02.31 PS.seek 0.000ml @09:18:28.55 PSV.dial :air @09:18:29.12 PS.seek 2.500ml @09:18:55.30 PSV.dial :mixloop @09:18:55.73 PS.seek 0.000ml @09:19:21.96 Garaging FlushPuck in Collection Clamp @09:19:22.02 Elbow.reconfigure TightElbow @09:19:22.05 Elbow.seek CC @09:19:22.14 Elbow.reconfigure LooseElbow @09:19:22.22 Forearm.seek CC @09:19:23.18 CC.seek sealed @09:19:24.13 Hand.grip @09:19:24.87 CC.seek open @09:19:28.18 Forearm.seek retract @09:19:29.17 Elbow.reconfigure TightElbow @09:19:29.21 Elbow.seek garage @09:19:32.04 Elbow.reconfigure LooseElbow @09:19:32.13 Forearm.reconfigure TightForearm @09:19:32.16 Forearm.seek garage @09:19:33.33 Hand.release @09:19:34.16 Forearm.reconfigure LooseForearm @09:19:34.19 Forearm.seek retract @09:19:35.22 Connecting PS to CS to transfer sample @09:19:35.23 CBV.dial AIV @09:19:35.24 AIV.dial :bypass @09:19:35.25 PSV.dial :mixloop @09:19:35.26 PTV.dial :PRVmixing PRV.dial AIV @09:19:35.27 CTV.dial :air,avoiding => intake @09:19:35.28 Loading lyfil puck from tube 2 into Collection Clamp @09:19:35.46 SC.reconfigure SCtight @09:19:35.49 SC.seek 2 @09:19:37.75 SC.reconfigure SCloose @09:19:37.92 Elbow.reconfigure TightElbow @09:19:37.96 Elbow.seek 2 @09:19:39.76 Forearm.reconfigure TightForearm @09:19:39.81 Forearm.seek 2 + 800 counts @09:19:40.89 SE.reconfigure SEpick @09:19:40.94 SE.seek beamBreak @09:19:42.18 SE.configure SEconfig @09:19:42.30 Elbow.seek 2 @09:19:42.44 Forearm.seek 2 + 300 counts @09:19:42.69 Forearm.reconfigure LooseForearm @09:19:42.73 Hand.grip @09:19:43.49 SE.seek down @09:19:43.64
Forearm.seek retract @09:19:44.57 SE.coast @09:19:44.62
Elbow.seek CC @09:19:46.17 Elbow.reconfigure LooseElbow @09:19:46.25 Forearm.reconfigure TightForearm @09:19:46.28 Forearm.seek CC @09:19:47.42 CC.seek closed @09:19:51.22 Collection Clamp onSpring/offSpring for 5/22 samples, on/off torque 1.98 @09:19:51.23 Hand.release @09:19:52.03 Forearm.reconfigure LooseForearm @09:19:52.06 Forearm.seek retract @09:19:52.98 SC.coast @09:19:53.01 CTV.dial CSV,avoiding => intake @09:19:54.31 CSV.dial CTV @09:19:54.60 CS.seek 5.000ml @09:20:08.06 delay 1:20 @09:21:28.08 CS.seek 2.500ml @09:21:35.26 delay 40 seconds @09:22:15.27 CS.seek 0.000ml @09:22:22.57 delay 1:00 @09:23:22.58 PSV.dial :air @09:23:23.03 PS.seek 2.500ml @09:23:49.24 PSV.dial :mixloop @09:23:49.67 delay 3 seconds @09:23:52.68 PSV.dial :air @09:23:53.11 delay 2 seconds @09:23:55.12 PSV.dial :mixloop @09:23:55.56 delay 3 seconds @09:23:58.57 PSV.dial :waste1 @09:23:59.85 PS.seek empty @09:24:26.14 CBV.dial :waste1 @09:24:26.61 delay 5 seconds @09:24:31.62 CBV.dial AIV @09:24:32.09 delay 10 seconds @09:24:42.10 CBV.dial :waste1 @09:24:42.57 delay 10 seconds @09:24:52.58 CBV.dial AIV @09:24:53.03 delay 10 seconds @09:25:03.04 CTV.dial CSV,avoiding => intake @09:25:03.05 CBV.dial between AIV and waste1 @09:25:03.37 CSV.dial :air @09:25:03.88 CS.seek 10.000ml @09:25:30.03 CSV.dial CTV @09:25:30.54 CS.seek 0.000ml @09:25:56.59 CBV.dial :waste1 @09:25:56.88 delay 10 seconds @09:26:06.89 CTV.dial :air,avoiding => intake @09:26:08.23 Storing lyfil puck in Collection Clamp to tube 1 @09:26:08.35 SC.reconfigure SCtight @09:26:08.38 SC.seek 1 @09:26:10.62 SC.reconfigure SCloose @09:26:10.79 Elbow.reconfigure TightElbow @09:26:10.83 Elbow.seek 1 @09:26:12.47 Forearm.reconfigure TightForearm @09:26:12.50 Forearm.seek 1 + 800 counts @09:26:13.57 SE.reconfigure SEpick @09:26:13.62 SE.seek beamBreak @09:26:16.29 SE.configure SEconfig @09:26:16.41 Elbow.reconfigure LimpElbow @09:26:16.44 Forearm.reconfigure LooseForearm @09:26:16.48 Forearm.seek 1 @09:26:16.69 Elbow.hold @09:26:16.74 Elbow.reconfigure TightElbow @09:26:16.82 SE.seek 3.04 Pucks below Top Plate @09:26:17.13 Forearm.coast @09:26:17.16 Forearm.seek 1 + 348 counts @09:26:17.39 Forearm.reconfigure TightForearm @09:26:17.43 Forearm.seek 1 + 800 counts @09:26:17.66 SE.seek 4.04 Pucks below Top Plate @09:26:18.19 Forearm.reconfigure LooseForearm @09:26:18.22 Forearm.seek retract @09:26:19.14 Elbow.seek CC @09:26:20.75 Elbow.reconfigure LooseElbow @09:26:20.84 Forearm.seek CC @09:26:21.80 CC.seek sealed @09:26:22.74 Hand.grip @09:26:23.48 CC.seek open @09:26:26.81 Forearm.seek retract @09:26:27.79 Elbow.reconfigure TightElbow @09:26:27.83 Elbow.seek 1 @09:26:29.42 Elbow.reconfigure LimpElbow @09:26:29.45 Forearm.seek 1 @09:26:30.49 Elbow.hold @09:26:30.54 Elbow.reconfigure TightElbow @09:26:30.58 Hand.release @09:26:31.44 Forearm.coast @09:26:31.47 Forearm.seek 1 + 316 counts @09:26:31.70 Forearm.reconfigure TightForearm @09:26:31.73 Forearm.seek 1 + 800 counts @09:26:32.07 SE.seek down @09:26:33.68
Forearm.reconfigure LooseForearm @09:26:33.71 Forearm.seek retract @09:26:34.39 SE.coast @09:26:34.67
SC.coast @09:26:34.69 PSV.dial :air @09:26:35.75 PS.seek 0.499ml @09:26:41.70 delay 3 seconds @09:26:44.72 PSV.dial :mixloop @09:26:45.15 PRV.dial :air @09:26:45.60 delay 3 seconds @09:26:48.61 PRV.dial CBV @09:26:49.06 CBV.dial AIV @09:26:49.52 PRV.dial between CBV and air @09:26:49.84 PRV.dial :air @09:26:50.19 PS.reconfigure PSslow @09:26:50.22 PS.seek 0.599ml @09:26:52.73 PRV.dial :waste2 @09:26:53.46 PS.reconfigure PSconfig @09:26:53.49 PS.seek empty @09:27:00.39 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @09:27:00.54 Elbow.seek FlushPuck::Garage @09:27:02.29 Elbow.reconfigure LooseElbow @09:27:02.38 Forearm.seek garage @09:27:03.39 Hand.grip @09:27:04.20 Forearm.seek retract @09:27:05.21 Elbow.reconfigure TightElbow @09:27:05.25 Elbow.seek CC @09:27:08.00 Elbow.reconfigure LooseElbow @09:27:08.09 Forearm.reconfigure TightForearm @09:27:08.12 Forearm.seek CC @09:27:09.22 CC.seek closed @09:27:12.96 Collection Clamp onSpring/offSpring for 5/22 samples, on/off torque 2.01 @09:27:13.01 Hand.release @09:27:13.77 Forearm.reconfigure LooseForearm @09:27:13.80 Forearm.seek retract @09:27:14.79 Clearing PML for modified lysate CTV.dial :air,avoiding => intake @09:27:14.80 PRV.dial :waste2 @09:27:14.81 PTV.dial :PRV,avoiding => puck @09:27:15.55 delay 2 seconds @09:27:17.55 PTV.dial :PRVmixing,avoiding => puck @09:27:18.27 delay 2 seconds @09:27:20.28 PSV.dial :PML @09:27:20.29 delay 2 seconds @09:27:22.29 PRV.dial :air @09:27:23.02 delay 2 seconds @09:27:25.08 PS.seek 1.499ml @09:27:41.23 delay 5 seconds @09:27:46.25 PS.seek 2.500ml @09:27:57.29 PSV.dial :air @09:27:57.86 PS.seek 0.000ml @09:28:24.10 mixing lysate with speDil @09:28:24.11 PSV.dial :PML @09:28:24.54 CBV.dial :PRV @09:28:24.55 PRV.dial :air @09:28:24.56 delay 2 seconds @09:28:26.57 PRV.dial :speDil @09:28:27.01 delay 2 seconds @09:28:29.07 PS.reconfigure PSslow1 @09:28:29.10 PS.seek 0.225ml @09:28:52.66 delay 5 seconds @09:28:57.67 PRV.dial CBV @09:28:58.40 delay 2 seconds @09:29:00.46 PS.seek 0.475ml @09:29:26.47 delay 5 seconds @09:29:31.48 PRV.dial :air @09:29:31.91 delay 2 seconds @09:29:33.92 PS.seek 0.925ml @09:30:20.50 delay 10 seconds @09:30:30.51 PRV.dial CBV @09:30:30.94 CTV.dial :air,avoiding => intake @09:30:30.95 PSV.dial :air @09:30:31.40 PS.reconfigure PSconfig @09:30:31.43 PS.seek 2.500ml @09:30:48.35 delay 3 seconds @09:30:51.36 PSV.dial PTV @09:30:52.08 PTV.dial :PRV,avoiding => puck @09:30:52.81 PS.seek 0.000ml @09:31:19.06 PSV.dial :air @09:31:19.79 PS.seek 2.500ml @09:31:45.97 delay 3 seconds @09:31:48.99 PSV.dial PTV @09:31:49.83 PTV.dial :PRV,avoiding => puck @09:31:49.85 PS.seek 0.000ml @09:32:16.12 Pushing modified lysate into AIV Injection Loop @09:32:16.13 CBV.dial AIV @09:32:16.14 AIV.dial :bypass @09:32:16.15 PRV.dial CBV @09:32:16.16 PSV.dial :air @09:32:17.43 PS.seek 2.500ml @09:32:43.65 delay 3 seconds @09:32:46.66 PSV.dial :PML @09:32:47.09 PTV.dial :PRVmixing,avoiding => puck @09:32:47.86 PS.reconfigure PSslow1 @09:32:47.89 PS.seek 1.850ml @09:33:54.28 delay 5 seconds @09:33:59.29 AIV.dial :sample @09:33:59.83 AWV.select :waste @09:33:59.98 PS.reconfigure PSslow2 @09:34:00.01 PS.seek 1.310ml @09:35:49.96 delay 15 seconds @09:36:04.97 AIV.dial :bypass @09:36:05.51 PS.reconfigure PSconfig @09:36:05.54 PS.seek 0.000ml @09:36:19.66 PSV.dial :air @09:36:20.11 PS.seek 2.500ml @09:36:46.36 PSV.dial :PML @09:36:46.81 PS.seek 0.000ml @09:37:13.02 AIV.dial :coilToMFB @09:37:13.03 AWV.select :air @09:37:13.17 Extracting Nucleic Acid from AIV @09:37:13.19
Beginning samplePassD @09:37:13.20 SPE.hold 2:46:40,55.0C @09:37:13.22 ASV.dial :SPEdelivery @09:37:13.34
AWV.select :air @09:37:13.35 AIV.dial :bypass @09:37:13.36 PSV.dial PSR @09:37:13.78 delay 20 seconds @09:37:14.39
PSR.select :killMFB @09:37:14.49 PS.seek 1.500ml @09:37:30.65 delay 5 seconds @09:37:33.80 primeLysateINT @09:37:33.81 AWV.select :air @09:37:33.82 AIV.dial :coilToMFB ATV.dial :AIV @09:37:35.18 AS.reconfigure ASslow1 @09:37:35.22 AS.seek 0.239ml @09:37:35.66
PSV.dial :waste1 @09:37:36.10 PS.seek 0.000ml @09:37:52.15 PSV.dial PSR @09:37:52.60 PSR.select :air @09:37:52.70 PS.seek 2.500ml @09:38:18.94 PRV.dial CBV @09:38:18.95
CBV.dial PRV @09:38:18.96 PSV.dial :mixloop @09:38:20.13 CTV.dial :air,avoiding => intake @09:38:20.14 PTV.dial :PRVmixing @09:38:20.15 PS.seek 0.000ml @09:38:24.46 delay 10 seconds @09:38:34.47 ATV.dial :air2 @09:38:35.07 AS.seek 0.269ml @09:38:41.44 ATV.dial :waste2 @09:38:41.81 AS.reconfigure ASnorm @09:38:41.84 AS.seek empty @09:38:46.40
PSV.dial PSR @09:38:47.56 PSR.select :flush @09:38:47.66 PS.seek 1.500ml @09:38:56.00 Lysate primed! @09:38:56.01 AFV.select :holdingCoil @09:38:56.02 ATV.dial :air2 @09:38:56.43 AS.reconfigure ASslow1 @09:38:56.46 AS.seek 0.069ml @09:39:03.81
delay 5 seconds @09:39:08.82 PSV.dial :waste1 @09:39:09.28 PS.seek 0.000ml @09:39:11.05 delay 3 seconds @09:39:14.06 ATV.dial :AIV @09:39:14.65 AS.seek 0.469ml @09:39:25.34
PSV.dial PSR @09:39:25.79 PSR.select :air @09:39:25.89 PS.seek 2.500ml @09:39:52.12 PRV.dial CBV @09:39:52.14
CBV.dial PRV @09:39:52.15 PSV.dial :mixloop @09:39:53.29 CTV.dial :air,avoiding => intake @09:39:53.30 PTV.dial :PRVmixing @09:39:53.32 PS.seek 0.000ml @09:40:19.55 PSV.dial PSR @09:40:20.57 PSR.select :flush @09:40:20.67 PS.seek 1.500ml @09:40:36.50 delay 5 seconds @09:40:36.71
delay 5 seconds @09:40:41.55 Solid Phase Extraction Heater at 51.6C @09:40:41.56 ATV.dial ASV @09:40:41.72
PSV.dial :waste1 @09:40:41.89 ASV.dial :SPEdelivery @09:40:41.95 AS.seek 0.041ml @09:40:42.17
PS.seek 0.000ml @09:40:58.27 PSV.dial PSR @09:40:58.72 PSR.select :air @09:40:58.82 PS.seek 2.500ml @09:41:25.02 PRV.dial CBV @09:41:25.04
CBV.dial PRV @09:41:25.05 PSV.dial :mixloop @09:41:26.05 CTV.dial :air,avoiding => intake @09:41:26.06 PTV.dial :PRVmixing @09:41:26.08 PS.seek 0.000ml @09:41:52.33 PSV.dial PSR @09:41:53.35 PSR.select :flush @09:41:53.45 PS.seek 1.500ml @09:42:09.50 delay 10 seconds @09:42:09.55
delay 5 seconds @09:42:14.56 PSV.dial :waste1 @09:42:15.12 PS.seek 0.000ml @09:42:19.51 rinseLysateLineINT @09:42:19.52 AFV.select :carrierSolution @09:42:19.61 delay 2 seconds @09:42:21.62 AS.reconfigure ASconfig @09:42:21.65 AS.seek 1.000ml @09:42:31.33
PSV.dial PSR @09:42:31.75 PSR.select :air @09:42:31.85 PS.seek 2.500ml @09:42:58.14 PRV.dial CBV @09:42:58.16
CBV.dial PRV @09:42:58.17 PSV.dial :mixloop @09:42:59.17 CTV.dial :air,avoiding => intake @09:42:59.18 PTV.dial :PRVmixing @09:42:59.19 PS.reconfigure PSslow @09:42:59.23 PS.seek 1.200ml @09:43:18.31 delay 1 second @09:43:19.32 AFV.select :holdingCoil @09:43:19.41 delay 1 second @09:43:20.42 ATV.dial :AIV @09:43:20.76 AIV.dial :coilToMFB @09:43:20.77 AWV.select :waste @09:43:20.88 AS.reconfigure ASslow0 @09:43:20.92 AS.seek 0.000ml @09:43:26.14
PSV.dial :waste1 @09:43:27.45 PS.reconfigure PSconfig @09:43:27.48 PS.seek 0.000ml @09:43:40.48 PSV.dial :air @09:43:41.51 PS.seek 2.500ml @09:44:07.76 PSV.dial :mixloop @09:44:08.21 PS.reconfigure PSslow @09:44:08.24 PS.seek 0.000ml @09:44:59.51 PSV.dial :air @09:45:00.14 PS.reconfigure PSconfig @09:45:00.17 PS.seek 2.500ml @09:45:03.24 ATV.dial :air2 @09:45:03.81 AS.reconfigure ASslow1 @09:45:03.84 AS.seek 0.050ml @09:45:14.24 ATV.dial :AIV @09:45:14.82 AS.seek 0.010ml @09:45:23.21 ATV.dial :waste @09:45:24.52 AS.seek empty @09:45:26.48
PSV.dial :mixloop @09:45:26.91 PS.reconfigure PSslow @09:45:26.94 PS.seek 0.000ml @09:45:27.09 ethanol wash @09:45:27.10 ATV.dial :air2 @09:45:28.06 AS.reconfigure ASslow2 @09:45:28.09 AS.seek 0.009ml @09:45:38.52 delay 3 seconds @09:45:41.53 Pulling 0.4ml of Ethanol Wash @09:45:41.54 ATV.dial :ethanol @09:45:42.12 AS.reconfigure ASslow1 @09:45:42.15 AS.seek 0.409ml @09:46:18.23
Cleaning Collection Syringe @09:46:18.24 CSV.dial CSR @09:46:18.67 CSR.select :flush @09:46:18.77 CS.seek 4.000ml @09:46:29.70 delay 5 seconds @09:46:34.71 CSR.select :air @09:46:34.80 CS.seek 10.000ml @09:46:50.85 CSV.dial CTV @09:46:50.87
CTV.dial CSV @09:46:52.20 CBV.dial :waste1 @09:46:52.66 CS.seek 0.000ml @09:47:03.97 delay 3 seconds @09:47:06.98 ATV.dial :air1 @09:47:07.30 delay 3 seconds @09:47:10.36 AS.seek 0.434ml @09:47:15.69 delay 3 seconds @09:47:18.70 ATV.dial :ethanol @09:47:18.82
CSV.dial CSR @09:47:19.16 AS.seek 0.419ml @09:47:19.93
CSR.select :flush @09:47:20.03 CS.seek 4.000ml @09:47:22.46 delay 3 seconds @09:47:25.47 ATV.dial ASV @09:47:26.62 AS.seek 0.092ml @09:47:31.04
delay 5 seconds @09:47:36.05 CSR.select :air @09:47:36.14 CS.seek 10.000ml @09:47:52.07 CSV.dial CTV @09:47:52.09
CTV.dial CSV @09:47:52.53 CBV.dial :waste1 @09:47:52.54 CS.seek 0.000ml @09:48:18.71 Garaging FlushPuck in Collection Clamp @09:48:18.76 Elbow.reconfigure TightElbow @09:48:18.79 Elbow.seek CC @09:48:18.88 Elbow.reconfigure LooseElbow @09:48:18.96 Forearm.seek CC @09:48:19.92 CC.seek sealed @09:48:20.85 Hand.grip @09:48:21.56 CC.seek open @09:48:24.85 Forearm.seek retract @09:48:25.79 Elbow.reconfigure TightElbow @09:48:25.83 Elbow.seek garage @09:48:28.65 Elbow.reconfigure LooseElbow @09:48:28.73 Forearm.reconfigure TightForearm @09:48:28.76 Forearm.seek garage @09:48:29.93 Hand.release @09:48:30.73 Forearm.reconfigure LooseForearm @09:48:30.76 Forearm.seek retract @09:48:31.78 Whole Cell Archive sampling 1000ml @09:48:31.79 Loading wcr puck from tube 2 into Collection Clamp @09:48:32.53 SC.reconfigure SCtight @09:48:32.56 SC.seek 2 @09:48:33.56 delay 3 seconds @09:48:34.73 SC.reconfigure SCloose @09:48:35.02 Elbow.reconfigure TightElbow @09:48:35.06 Elbow.seek 2 @09:48:36.57 ATV.dial :waste2 @09:48:36.90 Forearm.reconfigure TightForearm @09:48:36.93 Forearm.seek 2 + 800 counts @09:48:37.11 AS.reconfigure ASconfig @09:48:37.14 AS.seek empty @09:48:38.05 SE.reconfigure SEpick @09:48:38.10 SE.seek beamBreak @09:48:39.61 SE.configure SEconfig @09:48:39.74 Elbow.seek 2 @09:48:39.90 Forearm.seek 2 + 300 counts @09:48:40.21 Forearm.reconfigure LooseForearm @09:48:40.25 Hand.grip @09:48:40.96 SE.seek down @09:48:41.08 Forearm.seek retract @09:48:42.08 Elbow.seek CC @09:48:42.15 SE.coast @09:48:42.85 ATV.dial :air2 @09:48:43.20 AS.reconfigure ASslow1 @09:48:43.23 AS.seek 1.000ml @09:48:43.60 Elbow.reconfigure LooseElbow @09:48:43.68 Forearm.reconfigure TightForearm @09:48:43.71 Forearm.seek CC @09:48:44.85 CC.seek closed @09:48:48.66 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.95 @09:48:48.67 Hand.release @09:48:49.48 Forearm.reconfigure LooseForearm @09:48:49.51 Forearm.seek retract @09:48:50.47 SC.coast @09:48:50.51 collectSample 1000ml w/bubble point of 55psi @09:48:50.52 Priming 7ml @09:48:50.53 Sleepy.power :sampler,:ON Intake.select :close @09:48:50.54 Exhaust.select :close @09:48:50.55 SS.hold @09:48:50.71 Calibrating pressure sensors @09:48:50.72 CTV.dial :air @09:48:52.03 SSV.dial :spit,avoiding => exhaust @09:48:52.04 SS.reconfigure SSfast @09:48:52.07 SS.seek 5.00ml @09:48:54.84 SSV.dial CBV @09:48:54.86 CBV.dial SSV @09:48:56.26 delay 3.8 seconds @09:49:00.15 SSV.dial :spit @09:49:01.50 SS.seek 0.00ml @09:49:04.20 SSV.dial CBV @09:49:05.59 CTV.dial :intake @09:49:06.30 Waiting for external sampler to finish priming... @09:49:06.31 delayUntil "09:58:50.52" @09:52:07.64 delay 5 seconds @09:52:12.65 ATV.dial ASV @09:52:13.37 ASV.dial :SPEdelivery @09:52:13.39 AS.seek 0.020ml @09:55:33.58 delay 5 seconds @09:55:38.59 ATV.dial :air2 @09:55:39.47 AS.seek 0.520ml @09:57:21.73 delay 5 seconds @09:57:26.74 ATV.dial ASV @09:57:27.46 ASV.dial :wasteR @09:57:28.07 AS.seek 0.300ml @09:58:13.16 delay 20 seconds @09:58:33.17 AS.seek 0.070ml @09:58:50.53 SSV.dial CBV @09:58:50.55 CBV.dial SSV @09:58:50.56 Allowing 55s for 7.0ml of remaining 7.0ml @09:58:50.57 Intake.select :open @09:58:52.70 Intake @36.9psia, vacuum from 20.0 to 24.0psi @09:58:52.72 SS.reconfigure forSampling @09:58:52.75 SS.seek 7.00ml @09:58:58.31 Stroke completed -- waiting for pressure to equalize ... @09:58:58.95 Intake.select :close @09:58:59.08 SSV.dial :exhaust @09:58:59.87 Exhaust.select :open @09:58:59.97 SS.reconfigure SSexhale @09:59:00.01 SS.seek 0.00ml @09:59:03.43 Exhaust.select :close @09:59:03.54 Sampling 1000.0ml @09:59:04.11 Intake.select :close @09:59:04.12 Exhaust.select :close @09:59:04.13 SS.reconfigure SSconfig @09:59:04.16 SS.hold @09:59:04.32 CTV.dial :intake @09:59:04.34 CBV.dial SSV @09:59:04.35 SSV.dial CBV @09:59:05.16 Allowing 185s for 25.0ml of remaining 1000.0ml @09:59:05.17 Intake.select :open @09:59:07.30 Intake @36.4psia, vacuum from 20.0 to 24.0psi @09:59:07.32 SS.reconfigure forSampling @09:59:07.35 SS.seek 25.00ml @09:59:20.32 delay 20 seconds @09:59:21.45 Stroke completed -- waiting for pressure to equalize ... @09:59:22.09 Intake.select :close @09:59:22.23 SSV.dial :exhaust @09:59:23.03 Exhaust.select :open @09:59:23.13 SS.reconfigure SSexhale @09:59:23.17 SS.seek 0.00ml @09:59:32.07 Exhaust.select :close @09:59:32.17 SSV.dial CBV @09:59:32.19 CBV.dial SSV @09:59:32.99 Allowing 185s for 25.0ml of remaining 975.0ml @09:59:33.00 Intake.select :open @09:59:35.17 Intake @36.4psia, vacuum from 20.0 to 24.0psi @09:59:35.18 SS.reconfigure forSampling @09:59:35.21 SS.seek 25.00ml @09:59:40.33 ATV.dial :waste2 @09:59:40.89 AS.reconfigure ASconfig @09:59:40.92 AS.seek empty @09:59:45.35 Elution @09:59:45.36 ATV.dial :air1 @09:59:46.37 AS.reconfigure ASslow1 @09:59:46.40 AS.seek 0.049ml @09:59:48.31 Vacuum too high -- waiting up to 40s @09:59:48.58 Intake @27.6psia, vacuum from 20.0 to 24.0psi @09:59:48.59 SS.reconfigure forSampling @09:59:48.63 SS.seek 25.00ml @09:59:49.40 Stroke completed -- waiting for pressure to equalize ... @09:59:50.04 Intake.select :close @09:59:50.18 SSV.dial :exhaust @09:59:50.98 Exhaust.select :open @09:59:51.08 SS.reconfigure SSexhale @09:59:51.12 SS.seek 0.00ml @09:59:56.88 delay 3 seconds @09:59:59.89 ATV.dial :ElutionWater @10:00:00.06 Exhaust.select :close @10:00:00.16 SSV.dial CBV @10:00:00.18 CBV.dial SSV @10:00:00.48 AS.seek 0.109ml @10:00:00.99 Allowing 185s for 25.0ml of remaining 950.0ml @10:00:01.00 Intake.select :open @10:00:03.13 Intake @36.1psia, vacuum from 20.0 to 24.0psi @10:00:03.15 SS.reconfigure forSampling @10:00:03.18 SS.seek 25.00ml @10:00:11.75 Vacuum too high -- waiting up to 40s @10:00:12.01 Intake @29.6psia, vacuum from 20.0 to 24.0psi @10:00:12.03 SS.reconfigure forSampling @10:00:12.06 SS.seek 25.00ml @10:00:12.97 delay 3 seconds @10:00:15.98 ATV.dial ASV,via => air2 @10:00:17.82 ASV.dial :SPEelution @10:00:18.61 AFV.select :carrierSolution @10:00:18.76 AS.reconfigure ASnorm @10:00:18.79 AS.seek 0.759ml @10:00:19.95 Stroke completed -- waiting for pressure to equalize ... @10:00:20.59 Intake.select :close @10:00:20.73 SSV.dial :exhaust @10:00:21.56 Exhaust.select :open @10:00:21.67 SS.reconfigure SSexhale @10:00:21.71 SS.seek 0.00ml @10:00:30.54 Exhaust.select :close @10:00:30.64 SSV.dial CBV @10:00:30.66 CBV.dial SSV @10:00:31.48 Allowing 185s for 25.0ml of remaining 925.0ml @10:00:31.49 Intake.select :open @10:00:33.63 Intake @35.9psia, vacuum from 20.0 to 24.0psi @10:00:33.65 SS.reconfigure forSampling @10:00:33.68 SS.seek 25.00ml @10:00:36.65 Vacuum too high -- waiting up to 40s @10:00:36.92 Intake @30.5psia, vacuum from 20.0 to 24.0psi @10:00:36.94 SS.reconfigure forSampling @10:00:36.97 SS.seek 25.00ml @10:00:43.28 Vacuum too high -- waiting up to 40s @10:00:43.76 Intake @32.4psia, vacuum from 20.0 to 24.0psi @10:00:43.77 SS.reconfigure forSampling @10:00:43.81 SS.seek 25.00ml @10:00:46.99 Vacuum too high -- waiting up to 40s @10:00:47.47 Intake @33.1psia, vacuum from 20.0 to 24.0psi @10:00:47.48 SS.reconfigure forSampling @10:00:47.52 SS.seek 25.00ml @10:00:52.32 delay 3 seconds @10:00:52.68 Vacuum too high -- waiting up to 40s @10:00:53.17 Intake @34.0psia, vacuum from 20.0 to 24.0psi @10:00:53.18 SS.reconfigure forSampling @10:00:53.22 SS.seek 25.00ml @10:00:55.33 AFV.select :holdingCoil @10:00:55.47 AS.reconfigure ASslow1 @10:00:55.50 AS.seek 0.489ml @10:00:59.93 Stroke completed -- waiting for pressure to equalize ... @10:01:01.11 Intake.select :close @10:01:01.24 SSV.dial :exhaust @10:01:02.04 Exhaust.select :open @10:01:02.14 SS.reconfigure SSexhale @10:01:02.18 SS.seek 0.00ml @10:01:10.96 Exhaust.select :close @10:01:11.06 SSV.dial CBV @10:01:11.08 CBV.dial SSV @10:01:11.90 Allowing 185s for 25.0ml of remaining 900.0ml @10:01:11.91 Intake.select :open @10:01:14.05 Intake @36.4psia, vacuum from 20.0 to 24.0psi @10:01:14.07 SS.reconfigure forSampling @10:01:14.10 SS.seek 25.00ml @10:01:16.56 Vacuum too high -- waiting up to 40s @10:01:16.83 Intake @33.9psia, vacuum from 20.0 to 24.0psi @10:01:16.84 SS.reconfigure forSampling @10:01:16.88 SS.seek 25.00ml @10:01:35.99 Vacuum too high -- waiting up to 40s @10:01:36.26 Intake @33.9psia, vacuum from 20.0 to 24.0psi @10:01:36.28 SS.reconfigure forSampling @10:01:36.31 SS.seek 25.00ml @10:01:50.78 delay 5 seconds @10:01:55.84 AS.reconfigure ASslow2 @10:01:55.87 AS.seek 0.454ml @10:02:03.42 Stroke completed -- waiting for pressure to equalize ... @10:02:04.65 Intake.select :close @10:02:04.79 SSV.dial :exhaust @10:02:05.60 Exhaust.select :open @10:02:05.70 SS.reconfigure SSexhale @10:02:05.74 SS.seek 0.00ml @10:02:14.68 Exhaust.select :close @10:02:14.77 SSV.dial CBV @10:02:14.79 CBV.dial SSV @10:02:16.11 Allowing 185s for 25.0ml of remaining 875.0ml @10:02:16.12 Intake.select :open @10:02:18.26 Intake @35.8psia, vacuum from 20.0 to 24.0psi @10:02:18.28 SS.reconfigure forSampling @10:02:18.31 SS.seek 25.00ml @10:02:31.73 delay 1:00 @10:02:35.42 Vacuum too high -- waiting up to 40s @10:02:35.69 Intake @34.9psia, vacuum from 20.0 to 24.0psi @10:02:35.70 SS.reconfigure forSampling @10:02:35.74 SS.seek 25.00ml @10:03:31.74 pushing eluate to holding coil @10:03:31.80 AS.seek 0.294ml @10:03:54.62 Stroke completed -- waiting for pressure to equalize ... @10:03:55.85 Intake.select :close @10:03:55.99 SSV.dial :exhaust @10:03:56.79 Exhaust.select :open @10:03:56.89 SS.reconfigure SSexhale @10:03:56.93 SS.seek 0.00ml @10:04:05.51 Exhaust.select :close @10:04:05.61 SSV.dial CBV @10:04:05.63 CBV.dial SSV @10:04:06.43 Allowing 185s for 25.0ml of remaining 850.0ml @10:04:06.44 Intake.select :open @10:04:08.58 Intake @36.0psia, vacuum from 20.0 to 24.0psi @10:04:08.60 SS.reconfigure forSampling @10:04:08.63 SS.seek 25.00ml @10:04:35.17 Session Begun @10:04:41.92 -> showlog @10:05:44.95 -> exit Session Ended @10:05:51.17 Vacuum too high -- waiting up to 40s @10:05:51.65 Intake @35.1psia, vacuum from 20.0 to 24.0psi @10:05:51.66 SS.reconfigure forSampling @10:05:51.70 SS.seek 25.00ml @10:05:59.80 Stroke completed -- waiting for pressure to equalize ... @10:06:02.19 Intake.select :close @10:06:02.32 SSV.dial :exhaust @10:06:03.13 Exhaust.select :open @10:06:03.23 SS.reconfigure SSexhale @10:06:03.27 SS.seek 0.00ml @10:06:11.76 Exhaust.select :close @10:06:11.86 SSV.dial CBV @10:06:11.88 CBV.dial SSV @10:06:12.68 Allowing 185s for 25.0ml of remaining 825.0ml @10:06:12.69 Intake.select :open @10:06:14.84 Intake @35.5psia, vacuum from 20.0 to 24.0psi @10:06:14.85 SS.reconfigure forSampling @10:06:14.88 SS.seek 25.00ml @10:06:15.37 delay 30 seconds @10:06:26.70 Vacuum too high -- waiting up to 40s @10:06:27.17 Intake @34.8psia, vacuum from 20.0 to 24.0psi @10:06:27.18 SS.reconfigure forSampling @10:06:27.22 SS.seek 25.00ml @10:06:39.10 Vacuum too high -- waiting up to 40s @10:06:39.57 Intake @34.7psia, vacuum from 20.0 to 24.0psi @10:06:39.58 SS.reconfigure forSampling @10:06:39.62 SS.seek 25.00ml @10:06:45.38 ATV.dial :waste,via => air3 @10:06:46.94 AS.reconfigure ASconfig @10:06:46.97 AS.seek empty @10:06:48.38 Vacuum too high -- waiting up to 40s @10:06:49.44 Intake @35.1psia, vacuum from 20.0 to 24.0psi @10:06:49.45 SS.reconfigure forSampling @10:06:49.48 SS.seek 25.00ml @10:06:53.48 Vacuum too high -- waiting up to 40s @10:06:53.96 Intake @34.7psia, vacuum from 20.0 to 24.0psi @10:06:53.98 SS.reconfigure forSampling @10:06:54.01 SS.seek 25.00ml @10:06:57.79 Vacuum too high -- waiting up to 40s @10:06:58.27 Intake @35.3psia, vacuum from 20.0 to 24.0psi @10:06:58.29 SS.reconfigure forSampling @10:06:58.32 SS.seek 25.00ml @10:07:01.56 Vacuum too high -- waiting up to 40s @10:07:02.05 Intake @35.1psia, vacuum from 20.0 to 24.0psi @10:07:02.06 SS.reconfigure forSampling @10:07:02.10 SS.seek 25.00ml @10:07:04.48 Parking Bleach in LysateSource line and SPEcolumn @10:07:04.49 ATV.dial :air3 @10:07:04.82 Vacuum too high -- waiting up to 40s @10:07:05.12 AS.reconfigure ASslow1 @10:07:05.15 AS.seek 0.029ml @10:07:05.32 Intake @34.8psia, vacuum from 20.0 to 24.0psi @10:07:05.34 SS.reconfigure forSampling @10:07:05.37 SS.seek 25.00ml @10:07:08.38 Vacuum too high -- waiting up to 40s @10:07:08.88 SS.seek 25.00ml @10:07:11.54 Vacuum too high -- waiting up to 40s @10:07:11.60 ATV.dial :ElutionWater @10:07:12.09 Intake @34.9psia, vacuum from 20.0 to 24.0psi @10:07:12.11 SS.reconfigure forSampling @10:07:12.14 SS.seek 25.00ml @10:07:12.17 AS.seek 0.059ml @10:07:15.01 Vacuum too high -- waiting up to 40s @10:07:15.50 Intake @35.6psia, vacuum from 20.0 to 24.0psi @10:07:15.51 SS.reconfigure forSampling @10:07:15.55 SS.seek 25.00ml @10:07:17.79 Vacuum too high -- waiting up to 40s @10:07:18.27 Intake @35.1psia, vacuum from 20.0 to 24.0psi @10:07:18.28 SS.reconfigure forSampling @10:07:18.31 SS.seek 25.00ml @10:07:18.62 ATV.dial :air3 @10:07:19.03 AS.seek 0.069ml @10:07:20.76 Vacuum too high -- waiting up to 40s @10:07:21.25 Intake @35.1psia, vacuum from 20.0 to 24.0psi @10:07:21.27 SS.reconfigure forSampling @10:07:21.30 SS.seek 25.00ml @10:07:21.34 delay 2 seconds @10:07:23.35 ATV.dial :bleach @10:07:23.74 AS.seek 0.709ml @10:07:23.79 Vacuum too high -- waiting up to 40s @10:07:24.28 Intake @35.0psia, vacuum from 20.0 to 24.0psi @10:07:24.30 SS.reconfigure forSampling @10:07:24.33 SS.seek 25.00ml @10:07:26.82 Vacuum too high -- waiting up to 40s @10:07:27.31 Intake @34.4psia, vacuum from 20.0 to 24.0psi @10:07:27.32 SS.reconfigure forSampling @10:07:27.36 SS.seek 25.00ml @10:07:29.85 Vacuum too high -- waiting up to 40s @10:07:30.55 Intake @35.4psia, vacuum from 20.0 to 24.0psi @10:07:30.56 SS.reconfigure forSampling @10:07:30.60 SS.seek 25.00ml @10:07:33.01 Vacuum too high -- waiting up to 40s @10:07:33.50 Intake @35.2psia, vacuum from 20.0 to 24.0psi @10:07:33.52 SS.reconfigure forSampling @10:07:33.55 SS.seek 25.00ml @10:07:35.76 Vacuum too high -- waiting up to 40s @10:07:36.24 Intake @34.8psia, vacuum from 20.0 to 24.0psi @10:07:36.25 SS.reconfigure forSampling @10:07:36.29 SS.seek 25.00ml @10:07:38.82 Vacuum too high -- waiting up to 40s @10:07:39.53 Intake @35.5psia, vacuum from 20.0 to 24.0psi @10:07:39.54 SS.reconfigure forSampling @10:07:39.57 SS.seek 25.00ml @10:07:41.97 Vacuum too high -- waiting up to 40s @10:07:42.87 Intake @35.6psia, vacuum from 20.0 to 24.0psi @10:07:42.88 SS.reconfigure forSampling @10:07:42.91 SS.seek 25.00ml @10:07:45.60 Vacuum too high -- waiting up to 40s @10:07:45.87 Intake @34.9psia, vacuum from 20.0 to 24.0psi @10:07:45.89 SS.reconfigure forSampling @10:07:45.92 SS.seek 25.00ml @10:07:48.19 Vacuum too high -- waiting up to 40s @10:07:48.90 Intake @35.6psia, vacuum from 20.0 to 24.0psi @10:07:48.92 SS.reconfigure forSampling @10:07:48.95 SS.seek 25.00ml @10:07:51.22 Vacuum too high -- waiting up to 40s @10:07:51.82 Intake @34.9psia, vacuum from 20.0 to 24.0psi @10:07:51.83 SS.reconfigure forSampling @10:07:51.86 SS.seek 25.00ml @10:07:54.41 Vacuum too high -- waiting up to 40s @10:07:55.12 Intake @35.9psia, vacuum from 20.0 to 24.0psi @10:07:55.14 SS.reconfigure forSampling @10:07:55.17 SS.seek 25.00ml @10:07:57.16 Vacuum too high -- waiting up to 40s @10:07:57.65 Intake @35.4psia, vacuum from 20.0 to 24.0psi @10:07:57.67 SS.reconfigure forSampling @10:07:57.70 SS.seek 25.00ml @10:07:59.75 Vacuum too high -- waiting up to 40s @10:08:00.46 Intake @35.8psia, vacuum from 20.0 to 24.0psi @10:08:00.48 SS.reconfigure forSampling @10:08:00.51 SS.seek 25.00ml @10:08:02.44 Vacuum too high -- waiting up to 40s @10:08:02.92 Intake @35.2psia, vacuum from 20.0 to 24.0psi @10:08:02.94 SS.reconfigure forSampling @10:08:02.97 SS.seek 25.00ml @10:08:04.88 Vacuum too high -- waiting up to 40s @10:08:05.36 Intake @35.3psia, vacuum from 20.0 to 24.0psi @10:08:05.38 SS.reconfigure forSampling @10:08:05.41 SS.seek 25.00ml @10:08:07.41 Vacuum too high -- waiting up to 40s @10:08:08.11 Intake @36.4psia, vacuum from 20.0 to 24.0psi @10:08:08.13 SS.reconfigure forSampling @10:08:08.16 SS.seek 25.00ml @10:08:09.88 Vacuum too high -- waiting up to 40s @10:08:10.36 Intake @35.2psia, vacuum from 20.0 to 24.0psi @10:08:10.37 SS.reconfigure forSampling @10:08:10.41 SS.seek 25.00ml @10:08:12.35 Vacuum too high -- waiting up to 40s @10:08:12.84 Intake @35.0psia, vacuum from 20.0 to 24.0psi @10:08:12.86 SS.reconfigure forSampling @10:08:12.89 SS.seek 25.00ml @10:08:14.60 Vacuum too high -- waiting up to 40s @10:08:15.09 Intake @34.3psia, vacuum from 20.0 to 24.0psi @10:08:15.10 SS.reconfigure forSampling @10:08:15.14 SS.seek 25.00ml @10:08:17.13 Vacuum too high -- waiting up to 40s @10:08:17.82 Intake @34.2psia, vacuum from 20.0 to 24.0psi @10:08:17.84 SS.reconfigure forSampling @10:08:17.87 SS.seek 25.00ml @10:08:20.10 Vacuum too high -- waiting up to 40s @10:08:20.79 Intake @35.8psia, vacuum from 20.0 to 24.0psi @10:08:20.80 SS.reconfigure forSampling @10:08:20.84 SS.seek 25.00ml @10:08:22.63 Vacuum too high -- waiting up to 40s @10:08:23.10 Intake @34.9psia, vacuum from 20.0 to 24.0psi @10:08:23.11 SS.reconfigure forSampling @10:08:23.15 SS.seek 25.00ml @10:08:24.94 Vacuum too high -- waiting up to 40s @10:08:25.65 Intake @34.5psia, vacuum from 20.0 to 24.0psi @10:08:25.66 SS.reconfigure forSampling @10:08:25.70 SS.seek 25.00ml @10:08:27.85 Vacuum too high -- waiting up to 40s @10:08:28.77 Intake @34.3psia, vacuum from 20.0 to 24.0psi @10:08:28.79 SS.reconfigure forSampling @10:08:28.82 SS.seek 25.00ml @10:08:31.28 Vacuum too high -- waiting up to 40s @10:08:31.99 Intake @34.0psia, vacuum from 20.0 to 24.0psi @10:08:32.01 SS.reconfigure forSampling @10:08:32.04 SS.seek 25.00ml @10:08:34.28 Vacuum too high -- waiting up to 40s @10:08:34.99 Intake @34.9psia, vacuum from 20.0 to 24.0psi @10:08:35.01 SS.reconfigure forSampling @10:08:35.04 SS.seek 25.00ml @10:08:36.91 Vacuum too high -- waiting up to 40s @10:08:37.62 Intake @35.3psia, vacuum from 20.0 to 24.0psi @10:08:37.64 SS.reconfigure forSampling @10:08:37.67 SS.seek 25.00ml @10:08:38.98 Stroke completed -- waiting for pressure to equalize ... @10:08:41.38 Intake.select :close @10:08:41.52 SSV.dial :exhaust @10:08:42.33 Exhaust.select :open @10:08:42.43 SS.reconfigure SSexhale @10:08:42.47 SS.seek 0.00ml @10:08:51.18 Exhaust.select :close @10:08:51.28 SSV.dial CBV @10:08:51.30 CBV.dial SSV @10:08:52.11 Allowing 185s for 25.0ml of remaining 800.0ml @10:08:52.12 Intake.select :open @10:08:54.26 Intake @35.4psia, vacuum from 20.0 to 24.0psi @10:08:54.28 SS.reconfigure forSampling @10:08:54.31 SS.seek 25.00ml @10:08:57.68 Vacuum too high -- waiting up to 40s @10:08:58.17 Intake @34.5psia, vacuum from 20.0 to 24.0psi @10:08:58.18 SS.reconfigure forSampling @10:08:58.22 SS.seek 25.00ml @10:09:00.10 Vacuum too high -- waiting up to 40s @10:09:00.81 Intake @34.6psia, vacuum from 20.0 to 24.0psi @10:09:00.83 SS.reconfigure forSampling @10:09:00.86 SS.seek 25.00ml @10:09:03.22 Vacuum too high -- waiting up to 40s @10:09:03.93 Intake @34.7psia, vacuum from 20.0 to 24.0psi @10:09:03.94 SS.reconfigure forSampling @10:09:03.97 SS.seek 25.00ml @10:09:06.22 Vacuum too high -- waiting up to 40s @10:09:06.91 Intake @34.3psia, vacuum from 20.0 to 24.0psi @10:09:06.93 SS.reconfigure forSampling @10:09:06.96 SS.seek 25.00ml @10:09:09.22 Vacuum too high -- waiting up to 40s @10:09:09.92 Intake @34.9psia, vacuum from 20.0 to 24.0psi @10:09:09.94 SS.reconfigure forSampling @10:09:09.97 SS.seek 25.00ml @10:09:12.03 Vacuum too high -- waiting up to 40s @10:09:12.74 Intake @35.5psia, vacuum from 20.0 to 24.0psi @10:09:12.76 SS.reconfigure forSampling @10:09:12.79 SS.seek 25.00ml @10:09:14.69 Vacuum too high -- waiting up to 40s @10:09:15.18 Intake @34.5psia, vacuum from 20.0 to 24.0psi @10:09:15.19 SS.reconfigure forSampling @10:09:15.23 SS.seek 25.00ml @10:09:17.09 Vacuum too high -- waiting up to 40s @10:09:17.80 Intake @34.2psia, vacuum from 20.0 to 24.0psi @10:09:17.81 SS.reconfigure forSampling @10:09:17.85 SS.seek 25.00ml @10:09:19.91 Vacuum too high -- waiting up to 40s @10:09:20.62 Intake @34.4psia, vacuum from 20.0 to 24.0psi @10:09:20.63 SS.reconfigure forSampling @10:09:20.67 SS.seek 25.00ml @10:09:22.75 Vacuum too high -- waiting up to 40s @10:09:24.09 Intake @34.5psia, vacuum from 20.0 to 24.0psi @10:09:24.10 SS.reconfigure forSampling @10:09:24.13 SS.seek 25.00ml @10:09:26.81 Vacuum too high -- waiting up to 40s @10:09:27.52 Intake @34.9psia, vacuum from 20.0 to 24.0psi @10:09:27.54 SS.reconfigure forSampling @10:09:27.57 SS.seek 25.00ml @10:09:29.69 Vacuum too high -- waiting up to 40s @10:09:30.40 Intake @35.3psia, vacuum from 20.0 to 24.0psi @10:09:30.41 SS.reconfigure forSampling @10:09:30.45 SS.seek 25.00ml @10:09:32.09 Vacuum too high -- waiting up to 40s @10:09:32.80 Intake @34.3psia, vacuum from 20.0 to 24.0psi @10:09:32.82 SS.reconfigure forSampling @10:09:32.85 SS.seek 25.00ml @10:09:34.55 delay 3 seconds @10:09:35.13 Vacuum too high -- waiting up to 40s @10:09:35.84 Intake @35.0psia, vacuum from 20.0 to 24.0psi @10:09:35.85 SS.reconfigure forSampling @10:09:35.89 SS.seek 25.00ml @10:09:37.56 ATV.dial :air3 @10:09:37.66 Vacuum too high -- waiting up to 40s @10:09:37.92 delay 3 seconds @10:09:38.37 Intake @34.3psia, vacuum from 20.0 to 24.0psi @10:09:38.39 SS.reconfigure forSampling @10:09:38.42 SS.seek 25.00ml @10:09:40.53 Vacuum too high -- waiting up to 40s @10:09:41.03 AS.seek 0.734ml @10:09:41.27 Intake @34.8psia, vacuum from 20.0 to 24.0psi @10:09:41.29 SS.reconfigure forSampling @10:09:41.32 SS.seek 25.00ml @10:09:43.25 Vacuum too high -- waiting up to 40s @10:09:43.96 Intake @35.1psia, vacuum from 20.0 to 24.0psi @10:09:43.98 SS.reconfigure forSampling @10:09:44.01 SS.seek 25.00ml @10:09:45.78 Vacuum too high -- waiting up to 40s @10:09:46.35 delay 3 seconds @10:09:46.50 Intake @34.4psia, vacuum from 20.0 to 24.0psi @10:09:46.52 SS.reconfigure forSampling @10:09:46.55 SS.seek 25.00ml @10:09:48.66 Vacuum too high -- waiting up to 40s @10:09:49.38 SS.seek 25.00ml @10:09:49.41 ATV.dial :bleach @10:09:49.80 AS.seek 0.719ml @10:09:51.16 Vacuum too high -- waiting up to 40s @10:09:51.87 Intake @34.4psia, vacuum from 20.0 to 24.0psi @10:09:51.89 SS.reconfigure forSampling @10:09:51.92 SS.seek 25.00ml @10:09:53.13 delay 3 seconds @10:09:53.75 Vacuum too high -- waiting up to 40s @10:09:54.46 Intake @34.3psia, vacuum from 20.0 to 24.0psi @10:09:54.48 SS.reconfigure forSampling @10:09:54.51 SS.seek 25.00ml @10:09:56.14 ATV.dial :AIV @10:09:56.28 Vacuum too high -- waiting up to 40s @10:09:57.19 Intake @35.3psia, vacuum from 20.0 to 24.0psi @10:09:57.20 SS.reconfigure forSampling @10:09:57.24 SS.seek 25.00ml @10:09:57.29 AS.reconfigure ASconfig @10:09:57.33 AS.seek 0.015ml @10:09:59.06 Vacuum too high -- waiting up to 40s @10:09:59.77 Intake @35.2psia, vacuum from 20.0 to 24.0psi @10:09:59.78 SS.reconfigure forSampling @10:09:59.82 SS.seek 25.00ml @10:10:01.62 Vacuum too high -- waiting up to 40s @10:10:02.50 Intake @34.8psia, vacuum from 20.0 to 24.0psi @10:10:02.52 SS.reconfigure forSampling @10:10:02.55 SS.seek 25.00ml @10:10:04.53 Vacuum too high -- waiting up to 40s @10:10:05.24 Intake @34.7psia, vacuum from 20.0 to 24.0psi @10:10:05.26 SS.reconfigure forSampling @10:10:05.29 SS.seek 25.00ml @10:10:07.12 Vacuum too high -- waiting up to 40s @10:10:07.83 Intake @34.7psia, vacuum from 20.0 to 24.0psi @10:10:07.85 SS.reconfigure forSampling @10:10:07.88 SS.seek 25.00ml @10:10:09.56 Vacuum too high -- waiting up to 40s @10:10:10.27 Intake @34.4psia, vacuum from 20.0 to 24.0psi @10:10:10.29 SS.reconfigure forSampling @10:10:10.32 SS.seek 25.00ml @10:10:11.97 Vacuum too high -- waiting up to 40s @10:10:12.89 Intake @34.5psia, vacuum from 20.0 to 24.0psi @10:10:12.91 SS.reconfigure forSampling @10:10:12.94 SS.seek 25.00ml @10:10:14.97 Vacuum too high -- waiting up to 40s @10:10:15.89 Intake @35.5psia, vacuum from 20.0 to 24.0psi @10:10:15.91 SS.reconfigure forSampling @10:10:15.94 SS.seek 25.00ml @10:10:17.53 Vacuum too high -- waiting up to 40s @10:10:18.24 Intake @34.8psia, vacuum from 20.0 to 24.0psi @10:10:18.25 SS.reconfigure forSampling @10:10:18.29 SS.seek 25.00ml @10:10:20.09 Vacuum too high -- waiting up to 40s @10:10:20.80 Intake @34.7psia, vacuum from 20.0 to 24.0psi @10:10:20.82 SS.reconfigure forSampling @10:10:20.85 SS.seek 25.00ml @10:10:22.50 Vacuum too high -- waiting up to 40s @10:10:23.41 SS.seek 25.00ml @10:10:25.25 Vacuum too high -- waiting up to 40s @10:10:26.17 Intake @34.7psia, vacuum from 20.0 to 24.0psi @10:10:26.19 SS.reconfigure forSampling @10:10:26.22 SS.seek 25.00ml @10:10:28.09 Vacuum too high -- waiting up to 40s @10:10:29.01 Intake @34.4psia, vacuum from 20.0 to 24.0psi @10:10:29.02 SS.reconfigure forSampling @10:10:29.06 SS.seek 25.00ml @10:10:30.93 Vacuum too high -- waiting up to 40s @10:10:31.83 Intake @34.9psia, vacuum from 20.0 to 24.0psi @10:10:31.85 SS.reconfigure forSampling @10:10:31.88 SS.seek 25.00ml @10:10:33.59 Vacuum too high -- waiting up to 40s @10:10:34.51 Intake @35.3psia, vacuum from 20.0 to 24.0psi @10:10:34.52 SS.reconfigure forSampling @10:10:34.56 SS.seek 25.00ml @10:10:36.37 Vacuum too high -- waiting up to 40s @10:10:37.08 Intake @34.5psia, vacuum from 20.0 to 24.0psi @10:10:37.09 SS.reconfigure forSampling @10:10:37.12 SS.seek 25.00ml @10:10:38.64 ATV.dial :waste @10:10:38.87 Vacuum too high -- waiting up to 40s @10:10:40.21 AS.seek empty @10:10:40.30 Intake @35.1psia, vacuum from 20.0 to 24.0psi @10:10:40.31 SS.reconfigure forSampling @10:10:40.35 SS.seek 25.00ml @10:10:41.42 ATV.dial :air3 @10:10:42.00 AS.reconfigure ASslow1 @10:10:42.04 AS.seek 0.009ml @10:10:42.62 Vacuum too high -- waiting up to 40s @10:10:43.55 Intake @35.0psia, vacuum from 20.0 to 24.0psi @10:10:43.57 SS.reconfigure forSampling @10:10:43.60 SS.seek 25.00ml @10:10:44.39 delay 2 seconds @10:10:45.31 Vacuum too high -- waiting up to 40s @10:10:46.24 Intake @34.8psia, vacuum from 20.0 to 24.0psi @10:10:46.26 SS.reconfigure forSampling @10:10:46.29 SS.seek 25.00ml @10:10:46.40 ATV.dial :bleach @10:10:46.80 AS.seek 0.579ml @10:10:48.12 Vacuum too high -- waiting up to 40s @10:10:49.05 Intake @35.7psia, vacuum from 20.0 to 24.0psi @10:10:49.07 SS.reconfigure forSampling @10:10:49.10 SS.seek 25.00ml @10:10:50.59 Vacuum too high -- waiting up to 40s @10:10:51.29 Intake @34.8psia, vacuum from 20.0 to 24.0psi @10:10:51.31 SS.reconfigure forSampling @10:10:51.34 SS.seek 25.00ml @10:10:53.03 Vacuum too high -- waiting up to 40s @10:10:53.96 Intake @35.4psia, vacuum from 20.0 to 24.0psi @10:10:53.98 SS.reconfigure forSampling @10:10:54.01 SS.seek 25.00ml @10:10:55.78 Vacuum too high -- waiting up to 40s @10:10:56.71 Intake @35.2psia, vacuum from 20.0 to 24.0psi @10:10:56.73 SS.reconfigure forSampling @10:10:56.76 SS.seek 25.00ml @10:10:58.46 Vacuum too high -- waiting up to 40s @10:10:59.15 Intake @34.8psia, vacuum from 20.0 to 24.0psi @10:10:59.17 SS.reconfigure forSampling @10:10:59.20 SS.seek 25.00ml @10:11:00.81 Vacuum too high -- waiting up to 40s @10:11:01.72 Intake @34.7psia, vacuum from 20.0 to 24.0psi @10:11:01.73 SS.reconfigure forSampling @10:11:01.77 SS.seek 25.00ml @10:11:03.50 Vacuum too high -- waiting up to 40s @10:11:04.41 Intake @36.0psia, vacuum from 20.0 to 24.0psi @10:11:04.43 SS.reconfigure forSampling @10:11:04.46 SS.seek 25.00ml @10:11:05.87 Vacuum too high -- waiting up to 40s @10:11:06.56 Intake @35.1psia, vacuum from 20.0 to 24.0psi @10:11:06.58 SS.reconfigure forSampling @10:11:06.61 SS.seek 25.00ml @10:11:08.24 Vacuum too high -- waiting up to 40s @10:11:09.16 Intake @34.9psia, vacuum from 20.0 to 24.0psi @10:11:09.17 SS.reconfigure forSampling @10:11:09.21 SS.seek 25.00ml @10:11:10.87 Vacuum too high -- waiting up to 40s @10:11:11.79 Intake @34.7psia, vacuum from 20.0 to 24.0psi @10:11:11.80 SS.reconfigure forSampling @10:11:11.84 SS.seek 25.00ml @10:11:13.59 Vacuum too high -- waiting up to 40s @10:11:14.53 Intake @35.4psia, vacuum from 20.0 to 24.0psi @10:11:14.55 SS.reconfigure forSampling @10:11:14.58 SS.seek 25.00ml @10:11:16.21 Vacuum too high -- waiting up to 40s @10:11:17.14 Intake @35.0psia, vacuum from 20.0 to 24.0psi @10:11:17.16 SS.reconfigure forSampling @10:11:17.19 SS.seek 25.00ml @10:11:18.93 Vacuum too high -- waiting up to 40s @10:11:19.87 Intake @34.6psia, vacuum from 20.0 to 24.0psi @10:11:19.88 SS.reconfigure forSampling @10:11:19.91 SS.seek 25.00ml @10:11:21.75 Vacuum too high -- waiting up to 40s @10:11:22.69 SS.seek 25.00ml @10:11:24.44 Vacuum too high -- waiting up to 40s @10:11:25.59 Intake @35.4psia, vacuum from 20.0 to 24.0psi @10:11:25.61 SS.reconfigure forSampling @10:11:25.64 SS.seek 25.00ml @10:11:27.38 Vacuum too high -- waiting up to 40s @10:11:28.30 Intake @35.0psia, vacuum from 20.0 to 24.0psi @10:11:28.31 SS.reconfigure forSampling @10:11:28.35 SS.seek 25.00ml @10:11:30.07 Vacuum too high -- waiting up to 40s @10:11:31.01 SS.seek 25.00ml @10:11:32.75 Vacuum too high -- waiting up to 40s @10:11:33.67 Intake @35.3psia, vacuum from 20.0 to 24.0psi @10:11:33.69 SS.reconfigure forSampling @10:11:33.72 SS.seek 25.00ml @10:11:35.41 Vacuum too high -- waiting up to 40s @10:11:36.34 Intake @34.9psia, vacuum from 20.0 to 24.0psi @10:11:36.35 SS.reconfigure forSampling @10:11:36.39 SS.seek 25.00ml @10:11:38.03 Vacuum too high -- waiting up to 40s @10:11:39.16 Intake @35.8psia, vacuum from 20.0 to 24.0psi @10:11:39.17 SS.reconfigure forSampling @10:11:39.21 SS.seek 25.00ml @10:11:40.91 Vacuum too high -- waiting up to 40s @10:11:41.82 Intake @35.2psia, vacuum from 20.0 to 24.0psi @10:11:41.84 SS.reconfigure forSampling @10:11:41.87 SS.seek 25.00ml @10:11:43.57 Vacuum too high -- waiting up to 40s @10:11:44.47 Intake @35.5psia, vacuum from 20.0 to 24.0psi @10:11:44.48 SS.reconfigure forSampling @10:11:44.52 SS.seek 25.00ml @10:11:46.10 Vacuum too high -- waiting up to 40s @10:11:47.02 Intake @35.0psia, vacuum from 20.0 to 24.0psi @10:11:47.03 SS.reconfigure forSampling @10:11:47.07 SS.seek 25.00ml @10:11:48.69 Vacuum too high -- waiting up to 40s @10:11:50.31 Intake @35.2psia, vacuum from 20.0 to 24.0psi @10:11:50.33 SS.reconfigure forSampling @10:11:50.36 SS.seek 25.00ml @10:11:52.50 Vacuum too high -- waiting up to 40s @10:11:53.43 Intake @35.0psia, vacuum from 20.0 to 24.0psi @10:11:53.45 SS.reconfigure forSampling @10:11:53.48 SS.seek 25.00ml @10:11:55.13 Vacuum too high -- waiting up to 40s @10:11:56.28 Intake @36.2psia, vacuum from 20.0 to 24.0psi @10:11:56.30 SS.reconfigure forSampling @10:11:56.33 SS.seek 25.00ml @10:11:57.14 SS.reconfigure SSconfig @10:11:57.17 SS.hold @10:11:57.32 Intake.select :close @10:11:57.47 ShallowSampler::Clogged in WCR -- progress too slow after 224.5ml @10:11:57.48 SSV.dial :exhaust @10:11:58.29 Exhaust.select :open @10:11:58.39 SS.reconfigure SSexhale @10:11:58.43 SS.seek 0.00ml @10:12:07.11 Exhaust.select :close @10:12:07.22 Sampled 224.5ml @10:12:07.23 collectSample: Complete @10:12:07.24 Drying puck before fixing for 04:00.00 @10:12:07.25 CBV.dial :waste2b @10:12:08.05 delay 1:00 @10:12:10.21 Sleepy.power :sampler,:OFF @10:12:43.23 delay 3 seconds @10:12:46.24 ATV.dial :air3 @10:12:46.58 delay 3 seconds @10:12:49.64 AS.seek 0.604ml @10:12:55.02 delay 3 seconds @10:12:58.03 ATV.dial :bleach @10:12:58.42 AS.seek 0.589ml @10:13:01.71 delay 3 seconds @10:13:04.72 ATV.dial :ASV @10:13:05.62 ASV.dial :wasteR @10:13:06.14 AS.seek 0.000ml @10:13:08.05 CSV.dial :air @10:13:08.50 CS.reconfigure CSfast @10:13:08.53 CS.seek 10.000ml @10:13:32.61 CBV.dial between CSV and waste2b @10:13:32.92 Pumping wcr puck from tube 2 up to 55psi... @10:13:32.96 CBV.dial SSV @10:13:34.39 CBV.dial between CSV and waste2b @10:13:35.33 CSV.dial between air and CTV @10:13:35.72 CS.seek 6.722ml @10:13:44.06 CTV.dial CSV,avoiding => air @10:13:44.08 CSV.dial CTV @10:13:44.90 CS.reconfigure CSpressurize @10:13:44.93 CS.seek 0.000ml @10:14:02.49 CSV.dial :air @10:14:02.92 CS.reconfigure CSfast @10:14:02.95 CS.seek 10.000ml @10:14:26.87 CSV.dial between air and CTV @10:14:27.26 CS.seek 2.189ml @10:14:45.93 CTV.dial CSV,avoiding => air @10:14:45.94 CSV.dial CTV @10:14:46.23 CS.reconfigure CSpressurize @10:14:46.26 CS.seek 0.000ml @10:14:47.79 CBV.dial :waste2b @10:14:48.12 CS.reconfigure CSconfig @10:14:48.15 CS.seek empty @10:14:53.39 delay 1:00 @10:15:06.55 delay 1:00 @10:15:53.40 CTV.dial :air,avoiding => intake @10:15:54.68 Fix #1 of 2 (with rnal) for 02:00.00 @10:15:54.69 CS Pulling 2ml of rnal @10:15:54.70 CSV.dial CSR @10:15:55.12 CSR.select :rnal @10:15:55.22 CS.seek 2.000ml @10:16:01.11 delay 5 seconds @10:16:06.12 CSR.select :air @10:16:06.21 CS.reconfigure CSfast @10:16:06.25 CS.seek 10.000ml @10:16:06.56 AFV.select :carrierSolution @10:16:06.65 delay 2 seconds @10:16:08.66 AS.reconfigure ASconfig @10:16:08.69 AS.seek 1.000ml @10:16:25.54 CBV.dial between CSV and waste2b @10:16:25.87 Pumping wcr puck from tube 2 up to 55psi... @10:16:25.92 CBV.dial SSV @10:16:27.35 CBV.dial between CSV and waste2b @10:16:28.30 CSV.dial between air and CTV @10:16:28.96 CS.seek 6.687ml @10:16:37.43 CTV.dial CSV,avoiding => air @10:16:37.45 CSV.dial CTV @10:16:39.44 CS.reconfigure CSpressurize @10:16:39.48 CS.seek 0.000ml @10:16:56.47 delay 2:00 @10:17:07.26 delay 1 second @10:17:08.27 AFV.select :holdingCoil @10:17:08.36 delay 1 second @10:17:09.37 ATV.dial :ASV @10:17:09.38 ASV.dial :wasteR @10:17:09.39 AS.reconfigure ASslow1 @10:17:09.42 AS.seek 0.000ml @10:18:56.48 CBV.dial :waste2b @10:18:56.81 CS.reconfigure CSconfig @10:18:56.84 CS.seek empty @10:18:58.10 delay 1:00 @10:19:58.11 CTV.dial :air,avoiding => intake @10:19:59.37 Fix #2 of 2 (with rnal) for 02:00.00 @10:19:59.38 CS Pulling 2ml of rnal CSV.dial CSR @10:19:59.80 CSR.select :rnal @10:19:59.90 CS.seek 2.000ml @10:20:05.81 delay 5 seconds @10:20:10.82 CSR.select :air @10:20:10.91 CS.reconfigure CSfast @10:20:10.95 CS.seek 10.000ml @10:20:30.00 CBV.dial between CSV and waste2b @10:20:30.32 Pumping wcr puck from tube 2 up to 55psi... @10:20:30.36 CBV.dial SSV @10:20:31.79 CBV.dial between CSV and waste2b @10:20:32.74 CSV.dial between air and CTV @10:20:33.68 delay 20 seconds @10:20:33.81 CS.seek 6.569ml @10:20:42.45 CTV.dial CSV,avoiding => air @10:20:42.47 CSV.dial CTV @10:20:43.78 CS.reconfigure CSpressurize @10:20:43.81 CS.seek 0.000ml @10:20:53.69 ATV.dial :air3 @10:20:54.82 AS.seek 0.590ml @10:21:00.40 delay 2:00 @10:22:55.44 delay 3 seconds @10:22:58.45 ATV.dial :ASV @10:22:59.53 ASV.dial :wasteR @10:22:59.59 AS.seek 0.090ml @10:23:00.41 CBV.dial :waste2b @10:23:00.74 CS.reconfigure CSconfig @10:23:00.77 CS.seek empty @10:23:01.99 delay 1:00 @10:24:02.00 CTV.dial :air,avoiding => intake @10:24:03.32 Storing wcr puck in Collection Clamp to tube 1 @10:24:03.45 SC.reconfigure SCtight @10:24:03.48 SC.seek 1 @10:24:05.72 SC.reconfigure SCloose @10:24:05.90 Elbow.reconfigure TightElbow @10:24:05.93 Elbow.seek 1 @10:24:07.58 Forearm.reconfigure TightForearm @10:24:07.61 Forearm.seek 1 + 800 counts @10:24:08.64 SE.reconfigure SEpick @10:24:08.69 SE.seek beamBreak @10:24:11.29 SE.configure SEconfig @10:24:11.41 Elbow.reconfigure LimpElbow @10:24:11.45 Forearm.reconfigure LooseForearm @10:24:11.49 Forearm.seek 1 @10:24:11.71 Elbow.hold @10:24:11.76 Elbow.reconfigure TightElbow @10:24:11.84 SE.seek 4.04 Pucks below Top Plate @10:24:12.15 Forearm.coast @10:24:12.18 Forearm.seek 1 + 315 counts @10:24:12.42 Forearm.reconfigure TightForearm @10:24:12.45 Forearm.seek 1 + 800 counts @10:24:12.74 SE.seek 5.04 Pucks below Top Plate @10:24:13.30 Forearm.reconfigure LooseForearm @10:24:13.33 Forearm.seek retract @10:24:14.28 Elbow.seek CC @10:24:15.85 Elbow.reconfigure LooseElbow @10:24:15.93 Forearm.seek CC @10:24:16.88 CC.seek sealed @10:24:17.80 Hand.grip @10:24:18.49 CC.seek open @10:24:22.42 Forearm.seek retract @10:24:23.38 Elbow.reconfigure TightElbow @10:24:23.41 Elbow.seek 1 @10:24:25.03 Elbow.reconfigure LimpElbow @10:24:25.07 Forearm.seek 1 @10:24:26.05 Elbow.hold @10:24:26.10 Elbow.reconfigure TightElbow @10:24:26.14 Hand.release @10:24:26.94 Forearm.coast @10:24:26.98 Forearm.seek 1 + 328 counts @10:24:27.22 Forearm.reconfigure TightForearm @10:24:27.25 Forearm.seek 1 + 800 counts @10:24:27.58 SE.seek down @10:24:28.68 Forearm.reconfigure LooseForearm @10:24:28.71 Forearm.seek retract @10:24:29.81 SE.coast @10:24:29.84 SC.coast @10:24:29.87 Starting finalPurge @10:24:29.88 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @10:24:30.04 Elbow.seek FlushPuck::Garage @10:24:31.82 Elbow.reconfigure LooseElbow @10:24:31.90 Forearm.seek garage @10:24:32.93 Hand.grip @10:24:33.70 Forearm.seek retract @10:24:34.72 Elbow.reconfigure TightElbow @10:24:34.75 Elbow.seek CC @10:24:37.46 Elbow.reconfigure LooseElbow @10:24:37.54 Forearm.reconfigure TightForearm @10:24:37.57 Forearm.seek CC @10:24:38.70 CC.seek closed @10:24:41.83 delay 30 seconds @10:24:42.48
Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.97 @10:24:42.53 Hand.release @10:24:43.27 Forearm.reconfigure LooseForearm @10:24:43.30 Forearm.seek retract @10:24:44.22 Flushing the Collection Valve::Manifold @10:24:44.32 CS Pulling 5ml of flush CSV.dial CSR @10:24:44.74 CSR.select :flush @10:24:44.84 CS.seek 5.000ml @10:24:58.36 delay 5 seconds @10:25:03.37 CSR.select :air @10:25:03.51 CS.reconfigure CSfast @10:25:03.54 CS.seek 7.501ml @10:25:10.21 CSV.dial :waste @10:25:11.47 CS.reconfigure CSconfig @10:25:11.50 CS.seek 0.000ml @10:25:11.84 ATV.dial :extSrc @10:25:12.38 delay 3 seconds @10:25:15.38 ATV.dial :ElutionWater @10:25:16.63 AS.seek 0.120ml @10:25:23.00 ATV.dial :air3 @10:25:23.38 AS.seek 0.130ml @10:25:25.70 delay 2 seconds @10:25:27.71 ATV.dial :bleach @10:25:28.19 AS.seek 0.230ml @10:25:31.27
Flushing lower manifold's waste1 port @10:25:31.36 CSV.dial :flush @10:25:31.81 CS.reconfigure CSfast @10:25:31.84 CS.seek 5.000ml @10:25:44.17 delay 5 seconds @10:25:48.84 delay 3 seconds @10:25:49.18
CSV.dial :air @10:25:49.65 CS.seek 7.000ml @10:25:51.85 ATV.dial :air3 @10:25:52.19 delay 3 seconds @10:25:55.14 CSV.dial CTV @10:25:55.71
CTV.dial CSV @10:25:55.83 AS.seek 0.255ml @10:25:57.09
CBV.dial :waste1 @10:25:57.84 CS.seek 0.000ml @10:26:01.21 delay 3 seconds @10:26:04.22 ATV.dial :bleach @10:26:04.61 AS.seek 0.240ml @10:26:07.93 delay 3 seconds @10:26:10.94 ATV.dial :ASV @10:26:11.92 ASV.dial :SPEdelivery @10:26:12.39 AFV.select :carrierSolution @10:26:12.49 delay 2 seconds @10:26:14.50 AS.reconfigure ASconfig @10:26:14.54
Flushing lower manifold's waste2b port @10:26:14.57 AS.seek 0.500ml @10:26:14.69
CSV.dial :flush @10:26:15.40 CS.seek 5.000ml @10:26:27.61 delay 5 seconds @10:26:30.04 delay 1 second @10:26:31.05 AFV.select :holdingCoil @10:26:31.19 AS.reconfigure ASslow1 @10:26:31.22 AS.seek 0.170ml @10:26:32.62
CSV.dial :air @10:26:33.18 CS.seek 6.999ml @10:26:38.61 CSV.dial CTV @10:26:38.63
CTV.dial CSV @10:26:39.09 CBV.dial :waste2b @10:26:39.85 CS.seek 0.000ml @10:26:56.55 Flushing upper manifold @10:26:56.64 CSV.dial :flush @10:26:57.45 CS.seek 5.000ml @10:27:09.84 delay 5 seconds @10:27:14.85 CSV.dial :air @10:27:15.42 CS.seek 7.000ml @10:27:20.86 CSV.dial CBV @10:27:20.88
CBV.dial CSV @10:27:22.02 CTV.dial :air @10:27:23.29 CS.seek 0.000ml @10:27:38.83 SPE.hold 2:46:40,95.0C @10:27:38.85 delay 3:00 @10:27:40.01
delay 7 seconds @10:27:47.02 CS.seek 3.000ml @10:27:54.92 CSV.dial :waste @10:27:55.44 CS.seek 0.000ml @10:28:03.40 CSV.dial :flush @10:28:03.85 CS.seek 3.000ml @10:28:11.79 delay 5 seconds @10:28:16.80 CSV.dial :air @10:28:17.28 CS.seek 5.001ml @10:28:22.92 CSV.dial :air @10:28:22.94 CS.seek 0.000ml @10:28:35.52 delay 7 seconds @10:28:42.53 CS.seek 3.000ml @10:28:50.46 CSV.dial :waste @10:28:51.28 CS.seek 0.000ml @10:28:59.14 Disinfecting external Intake with mfbkill @10:28:59.23 CSV.dial CSR @10:29:00.53 CSR.select :mfbkill @10:29:00.63 CS.seek 8.000ml @10:29:20.49 delay 5 seconds @10:29:25.50 CSR.select :air @10:29:25.59 CS.seek 10.000ml @10:29:31.27 CSV.dial CBV @10:29:31.29
CBV.dial CSV @10:29:32.39 CTV.dial :intake @10:29:33.11 Intake.select :open @10:29:33.21 CS.seek 2.000ml @10:29:53.14 Intake.select :close @10:29:53.23 CTV.dial :air @10:29:54.00 CS.reconfigure CSconfig @10:29:54.03 CS.seek 0.000ml @10:29:59.98 Flushing lower manifold's waste1 port @10:29:59.99 CS Pulling 4ml of mfbkill @10:30:00.00 CSV.dial CSR @10:30:01.08 CSR.select :mfbkill @10:30:01.18 CS.seek 4.000ml @10:30:12.20 delay 5 seconds @10:30:17.21 CSR.select :air @10:30:17.30 CS.reconfigure CSfast @10:30:17.34 CS.seek 10.000ml @10:30:32.37 CSV.dial CTV @10:30:32.39
CTV.dial CSV @10:30:33.78 CBV.dial :waste1 @10:30:34.24 CS.seek 0.000ml @10:30:38.87 AFV.select :carrierSolution @10:30:38.96 delay 2 seconds @10:30:40.97 AS.reconfigure ASconfig @10:30:41.00 AS.seek 1.000ml @10:30:58.23
Flushing lower manifold's waste2b port @10:30:58.24 CS Pulling 4ml of mfbkill @10:30:58.25 CSV.dial CSR @10:30:58.76 CSR.select :mfbkill @10:30:58.86 CS.reconfigure CSconfig @10:30:58.90 CS.seek 4.000ml @10:31:09.83 delay 5 seconds @10:31:14.84 CSR.select :air @10:31:15.40 CS.reconfigure CSfast @10:31:15.44 CS.seek 10.000ml @10:31:29.67 delay 1 second @10:31:30.40 CSV.dial CTV @10:31:30.42
CTV.dial CSV @10:31:30.68 AFV.select :holdingCoil @10:31:30.77 delay 1 second @10:31:30.94
CBV.dial :waste2b @10:31:31.69 CS.seek 0.000ml @10:31:31.78 ASV.dial :wasteD @10:31:33.07 AS.seek 0.000ml @10:31:55.54
Killing upper manifold @10:31:55.55 CS Pulling 4ml of mfbkill @10:31:55.56 CSV.dial CSR @10:31:56.07 CSR.select :mfbkill @10:31:56.17 CS.reconfigure CSconfig @10:31:56.21 CS.seek 4.000ml @10:32:07.14 delay 5 seconds @10:32:12.15 CSR.select :air @10:32:12.24 CS.reconfigure CSfast @10:32:12.28 CS.seek 10.000ml @10:32:26.79 CSV.dial CBV @10:32:26.81
CBV.dial CSV @10:32:28.07 CTV.dial :air @10:32:29.36 CS.seek 0.000ml @10:32:31.63 Priming Eluate @10:32:31.64 AFV.select :carrierSolution @10:32:31.73 delay 2 seconds @10:32:33.74 AS.seek 0.500ml @10:32:52.85
delay 7 seconds @10:32:59.86 CS.seek 3.000ml @10:33:03.16 delay 1 second @10:33:04.17 AFV.select :holdingCoil @10:33:04.26 ATV.dial ASV @10:33:04.27 ASV.dial :SPErecovery @10:33:04.77 Swish #1 of 2 @10:33:04.82 AS.reconfigure ASslow0 @10:33:04.86 AS.seek 0.600ml @10:33:07.63
CSV.dial :waste @10:33:08.16 CS.seek 0.000ml @10:33:15.34 AS.seek 0.500ml @10:33:15.85
Flushing upper manifold @10:33:15.94 CSV.dial :flush @10:33:16.39 CS.seek 5.000ml @10:33:25.80 Swish #2 of 2 @10:33:25.86 AS.seek 0.600ml @10:33:28.63
delay 5 seconds @10:33:33.64 CSV.dial :air @10:33:34.12 CS.seek 7.000ml @10:33:36.35 AS.seek 0.500ml @10:33:39.60 CSV.dial CBV @10:33:39.62
CBV.dial CSV @10:33:41.30 CTV.dial :air @10:33:41.32 CS.seek 0.000ml @10:33:46.82 delay 3 seconds @10:33:49.88 AS.reconfigure ASslow1 @10:33:49.91 AS.seek 0.676ml @10:33:57.97
delay 7 seconds @10:34:04.98 CS.seek 3.000ml @10:34:12.66 CSV.dial :waste @10:34:13.09 CS.seek 0.000ml @10:34:20.83 CSV.dial :flush @10:34:21.28 CS.seek 3.000ml @10:34:26.11 delay 3 seconds @10:34:29.00
delay 5 seconds @10:34:29.12 Eluate primed @10:34:29.13 ATV.dial :waste2 @10:34:29.67 AS.reconfigure ASnorm @10:34:29.70 AS.seek 0.000ml @10:34:34.01
CSV.dial :air @10:34:34.47 CS.seek 4.999ml @10:34:39.94 CSV.dial :air @10:34:39.96 CS.seek 0.000ml @10:34:52.10 delay 7 seconds @10:34:59.12 CS.seek 3.000ml @10:35:04.58 SPE.coast @10:35:04.66 ASV.dial :SPErecovery @10:35:04.68 Setting up PCR for HF183em -- writing /var/log/esp/HF183em14mar0510h274ml.pcr @10:35:04.69 Building PCR Slug from ASV with MM2 mix, PP4 primer @10:35:04.70 Priming with carrier ATV.dial ARV @10:35:05.41 ARV.dial :carrier @10:35:05.69 AS.seek 0.500ml @10:35:06.85
CSV.dial :waste @10:35:07.64 CS.seek 0.000ml @10:35:15.19 Flushing lower manifold's waste1 port @10:35:15.28 CSV.dial :flush @10:35:15.79 CS.seek 5.000ml @10:35:27.94 delay 5 seconds @10:35:31.57 AFV.select :carrierSolution @10:35:31.68 delay 2 seconds @10:35:32.95
CSV.dial :air @10:35:33.43 CS.seek 7.000ml @10:35:33.69 AS.reconfigure ASconfig @10:35:33.73 AS.seek 1.000ml @10:35:38.88 CSV.dial CTV @10:35:38.90
CTV.dial CSV @10:35:40.22 CBV.dial :waste1 @10:35:40.67 CS.seek 0.000ml @10:35:57.25 Flushing lower manifold's waste2b port @10:35:57.34 CSV.dial :flush @10:35:58.13 CS.seek 5.000ml @10:36:03.16 delay 1 second @10:36:04.17 AFV.select :holdingCoil @10:36:04.27 delay 1 second @10:36:05.28 ATV.dial :waste @10:36:06.56 AS.reconfigure ASnorm @10:36:06.59 AS.seek empty @10:36:10.75
delay 5 seconds @10:36:15.76 CSV.dial :air @10:36:16.31 CS.seek 7.000ml @10:36:21.81 CSV.dial CTV @10:36:21.83
CTV.dial CSV @10:36:22.27 CBV.dial :waste2b @10:36:23.01 CS.seek 0.000ml @10:36:39.62 Disinfecting external Intake with kill @10:36:39.72 CSV.dial CSR @10:36:40.14 CSR.select :kill @10:36:40.24 CS.seek 8.000ml @10:36:58.08 AFV.select :carrierSolution @10:36:58.19 delay 2 seconds @10:36:59.31
delay 5 seconds @10:37:00.20 AS.reconfigure ASconfig @10:37:00.24 AS.seek 0.500ml @10:37:04.32
CSR.select :air @10:37:04.41 CS.seek 10.000ml @10:37:09.78 CSV.dial CBV @10:37:09.80
CBV.dial CSV @10:37:10.83 CTV.dial :intake @10:37:11.57 Intake.select :open @10:37:11.66 CS.seek 2.000ml @10:37:29.73 delay 1 second @10:37:30.74 AFV.select :holdingCoil @10:37:30.81
Intake.select :close @10:37:30.85 Building Slug @10:37:30.86 ATV.dial :ARV @10:37:30.90
CTV.dial :air @10:37:31.63 CS.reconfigure CSconfig @10:37:31.66 CS.seek 0.000ml @10:37:32.13 delay 2 seconds @10:37:34.13 ARV.dial :air1 @10:37:34.46 delay 2 seconds @10:37:36.51 AS.reconfigure ASslow2 @10:37:36.54 AS.seek 0.506ml @10:37:37.56
CSV.dial CSR @10:37:38.60 CSR.select :flush @10:37:38.70 CS.seek 5.000ml @10:37:42.98 delay 2 seconds @10:37:44.99 ARV.dial :MM2 @10:37:46.30 delay 2 seconds @10:37:48.31 Adding MM2 @10:37:48.36 AS.seek 0.524ml @10:37:52.18
delay 5 seconds @10:37:57.19 CSR.select :air @10:37:57.28 CS.seek 10.000ml @10:38:06.94 delay 25 seconds @10:38:10.68
CSV.dial CBV @10:38:11.74 CS.seek 0.000ml @10:38:31.95 ARV.dial :PP4 @10:38:32.28 Adding PP4 @10:38:32.29 delay 2 seconds @10:38:34.35 AS.seek 0.530ml @10:38:37.90
Garaging FlushPuck in Collection Clamp @10:38:37.96 Elbow.reconfigure TightElbow @10:38:37.99 Elbow.seek CC @10:38:38.08 Elbow.reconfigure LooseElbow @10:38:38.17 Forearm.seek CC @10:38:39.11 CC.seek sealed @10:38:40.05 Hand.grip @10:38:40.68 delay 10 seconds @10:38:40.83
CC.seek open @10:38:44.18 Forearm.seek retract @10:38:45.12 Elbow.reconfigure TightElbow @10:38:45.16 Elbow.seek garage @10:38:47.86 Elbow.reconfigure LooseElbow @10:38:47.94 Forearm.reconfigure TightForearm @10:38:47.97 Forearm.seek garage @10:38:49.13 Hand.release @10:38:49.87 Forearm.reconfigure LooseForearm @10:38:49.91 Forearm.seek retract @10:38:50.69 ARV.dial :air3 @10:38:51.13 adding ARV air @10:38:51.14 delay 2 seconds @10:38:53.19 AS.seek 0.536ml @10:38:59.62 delay 5 seconds @10:39:04.63 ARV.dial :carrier @10:39:05.67 delay 7 seconds @10:39:12.73 AS.seek 0.594ml @10:40:12.01 delay 5 seconds @10:40:17.02 ATV.dial :ASV @10:40:17.43 delay 2 seconds @10:40:19.49 AS.seek 0.600ml @10:40:25.74 delay 4 seconds @10:40:29.75 ATV.dial ARV @10:40:30.07 delay 2 seconds @10:40:32.08 ARV.dial :carrier @10:40:32.09 delay 2 seconds @10:40:34.14 AS.seek 0.650ml @10:41:25.83 delay 4 seconds @10:41:29.84 ATV.dial PCR @10:41:30.60 ASV.dial :waste1 @10:41:31.55 ARV.dial :air1 @10:41:31.88 Positioning Slug by 0.324ml @10:41:31.93 AS.seek 0.326ml @10:47:02.80 delay 5 seconds @10:47:07.81 ATV.dial ARV @10:47:08.32 ASV.dial :PCRlock @10:47:08.80 delay 5 seconds @10:47:13.81 Running HF183em ProfileEM60C2 thermocycling @10:47:13.82 PCR.program ProfileEM60C2 @10:47:13.93 ProfileEM60C2 should complete in < 2:11:38 @10:47:13.94 PCR.run 2:49:32.5 @12:42:44.36 PCR.off @12:42:44.89 PCR for HF183em completed -- closed /var/log/esp/HF183em14mar0510h274ml.pcr @12:42:44.94 Cleaning PCR @12:42:44.95 AFV.select :carrierSolution @12:42:45.04 delay 2 seconds @12:42:47.05 AS.reconfigure ASconfig @12:42:47.08 AS.seek 0.500ml @12:42:57.49 delay 1 second @12:42:58.50 AFV.select :holdingCoil @12:42:58.59 ASV.dial :waste1 @12:42:59.34 ATV.dial :air3 @12:43:00.29 AS.reconfigure ASslow1 @12:43:00.32 AS.seek 0.510ml @12:43:02.62 delay 2 seconds @12:43:04.63 ATV.dial :bleach @12:43:05.03 AS.seek 0.810ml @12:44:06.48 delay 3 seconds @12:44:09.49 ATV.dial :air3 @12:44:09.84 delay 3 seconds @12:44:12.90 AS.seek 0.835ml @12:44:18.23 delay 3 seconds @12:44:21.24 ATV.dial :bleach @12:44:21.64 AS.seek 0.820ml @12:44:24.96 delay 3 seconds @12:44:27.97 ARV.dial :waste @12:44:28.48 delay 3 seconds @12:44:31.49 ATV.dial :ARV @12:44:32.31 AS.reconfigure ASconfig @12:44:32.35 AS.seek 0.470ml @12:44:53.05 AFV.select :carrierSolution @12:44:53.14 delay 2 seconds @12:44:55.15 AS.seek 1.000ml @12:45:26.44 delay 1 second @12:45:27.45 AFV.select :holdingCoil @12:45:27.54 delay 1 second @12:45:28.55 AS.reconfigure ASfast @12:45:28.58 AS.seek 0.000ml @12:45:56.33 AFV.select :carrierSolution @12:45:56.42 delay 2 seconds @12:45:58.43 AS.reconfigure ASconfig @12:45:58.46 AS.seek 1.000ml @12:46:57.62 delay 1 second @12:46:58.63 AFV.select :holdingCoil @12:46:58.72 delay 1 second @12:46:59.73 AS.reconfigure ASfast @12:46:59.76 AS.seek 0.000ml @12:47:27.48 AFV.select :carrierSolution @12:47:27.58 delay 2 seconds @12:47:29.59 AS.reconfigure ASconfig @12:47:29.62 AS.seek 1.000ml @12:48:28.20 delay 1 second @12:48:29.21 AFV.select :holdingCoil @12:48:29.30 delay 1 second @12:48:30.31 AS.reconfigure ASfast @12:48:30.34 AS.seek 0.000ml @12:48:58.04 ARV.dial between carrier and air1 @12:48:58.55 ATV.dial :air3 @12:48:59.50 AS.reconfigure ASslow1 @12:48:59.53 AS.seek 0.010ml @12:49:01.88 delay 2 seconds @12:49:03.89 ATV.dial :bleach @12:49:04.37 AS.seek 0.810ml @12:51:47.77 delay 3 seconds @12:51:50.78 ATV.dial :air3 @12:51:51.11 delay 3 seconds @12:51:54.17 AS.seek 0.835ml @12:51:59.52 delay 3 seconds @12:52:02.53 ATV.dial :bleach @12:52:02.93 AS.seek 0.820ml @12:52:06.25 delay 3 seconds @12:52:09.26 ATV.dial PCR @12:52:09.57 AS.reconfigure ASconfig @12:52:09.60 AS.seek empty @12:52:57.72 PCR.program :bleachProfile @12:52:57.77 PCR program should complete in < 2:45 @12:52:57.78 PCR.run 10:00 @12:55:58.99 PCR.off @12:55:59.02 AFV.select :carrierSolution @12:55:59.11 delay 2 seconds @12:56:01.12 AS.seek 1.000ml @12:56:59.77 delay 1 second @12:57:00.78 AFV.select :holdingCoil @12:57:00.87 delay 1 second @12:57:01.88 AS.reconfigure ASfast @12:57:01.91 AS.seek 0.000ml @12:57:29.65 delay 3 seconds @12:57:32.66 AFV.select :carrierSolution @12:57:32.75 delay 2 seconds @12:57:34.76 AS.reconfigure ASconfig @12:57:34.80 AS.seek 1.000ml @12:58:33.43 delay 1 second @12:58:34.44 AFV.select :holdingCoil @12:58:34.53 delay 1 second @12:58:35.54 AS.reconfigure ASfast @12:58:35.57 AS.seek 0.000ml @12:59:03.28 delay 3 seconds @12:59:06.29 AFV.select :carrierSolution @12:59:06.38 delay 2 seconds @12:59:08.39 AS.reconfigure ASconfig @12:59:08.42 AS.seek 1.000ml @13:00:07.05 delay 1 second @13:00:08.06 AFV.select :holdingCoil @13:00:08.15 delay 1 second @13:00:09.16 AS.reconfigure ASfast @13:00:09.19 AS.seek 0.000ml @13:00:36.89 delay 3 seconds @13:00:39.90 AFV.select :carrierSolution @13:00:39.99 delay 2 seconds @13:00:42.00 AS.reconfigure ASconfig @13:00:42.03 AS.seek 1.000ml @13:01:40.64 delay 1 second @13:01:41.65 AFV.select :holdingCoil @13:01:41.74 delay 1 second @13:01:42.75 AS.reconfigure ASfast @13:01:42.78 AS.seek 0.000ml @13:02:10.49 delay 3 seconds @13:02:13.50 AFV.select :carrierSolution @13:02:13.59 delay 2 seconds @13:02:15.60 AS.reconfigure ASconfig @13:02:15.63 AS.seek 1.000ml @13:03:14.21 delay 1 second @13:03:15.22 AFV.select :holdingCoil @13:03:15.33 delay 1 second @13:03:16.34 AS.reconfigure ASnorm @13:03:16.37 AS.seek 0.000ml @13:04:07.85 Setting up PCR for ENTap -- writing /var/log/esp/ENTap14mar0513h274ml.pcr @13:04:07.86 Building PCR Slug from ASV with MM1 mix, PP1 primer @13:04:07.87 Priming with carrier @13:04:07.88 ATV.dial ARV @13:04:08.39 ARV.dial :carrier @13:04:08.66 AS.seek 0.500ml @13:04:34.55 AFV.select :carrierSolution @13:04:34.64 delay 2 seconds @13:04:36.65 AS.reconfigure ASconfig @13:04:36.69 AS.seek 1.000ml @13:05:06.11 delay 1 second @13:05:07.12 AFV.select :holdingCoil @13:05:07.21 delay 1 second @13:05:08.22 ATV.dial :waste @13:05:09.50 AS.reconfigure ASnorm @13:05:09.53 AS.seek empty @13:06:01.02 AFV.select :carrierSolution @13:06:01.12 delay 2 seconds @13:06:03.13 AS.reconfigure ASconfig @13:06:03.16 AS.seek 0.500ml @13:06:32.64 delay 1 second @13:06:33.65 AFV.select :holdingCoil @13:06:33.74 Building Slug @13:06:33.75 ATV.dial :ARV @13:06:35.09 delay 2 seconds @13:06:37.10 ARV.dial :air1 @13:06:37.42 delay 2 seconds @13:06:39.48 AS.reconfigure ASslow2 @13:06:39.51 AS.seek 0.506ml @13:06:45.84 delay 2 seconds @13:06:47.85 ARV.dial :MM1 @13:06:48.34 delay 2 seconds @13:06:50.35 Adding MM1 @13:06:50.41 AS.seek 0.524ml @13:07:09.29 delay 25 seconds @13:07:34.30 ARV.dial :PP1 @13:07:34.63 Adding PP1 @13:07:34.64 delay 2 seconds @13:07:36.69 AS.seek 0.530ml @13:07:43.06 delay 10 seconds @13:07:53.07 ARV.dial :air1 @13:07:53.40 adding ARV air @13:07:53.41 delay 2 seconds @13:07:55.96 AS.seek 0.536ml @13:08:02.50 delay 5 seconds @13:08:07.51 ARV.dial :carrier @13:08:07.82 delay 7 seconds @13:08:14.88 AS.seek 0.594ml @13:09:14.34 delay 5 seconds @13:09:19.35 ATV.dial :ASV @13:09:19.68 delay 2 seconds @13:09:21.74 AS.seek 0.600ml @13:09:28.12 delay 4 seconds @13:09:32.13 ATV.dial ARV @13:09:32.46 delay 2 seconds @13:09:34.47 ARV.dial :carrier @13:09:34.48 delay 2 seconds @13:09:36.53 AS.seek 0.650ml @13:10:27.93 delay 4 seconds @13:10:31.94 ATV.dial PCR @13:10:32.44 ASV.dial :waste1 @13:10:32.45 ARV.dial :air1 @13:10:32.80 Positioning Slug by 0.324ml @13:10:32.85 AS.seek 0.326ml @13:13:12.27 Session Begun @13:13:12.30 -> Sleepy.cycleModemPower! 9 @13:13:12.32 Session Ended @13:16:03.46 delay 5 seconds @13:16:08.47 ATV.dial ARV @13:16:08.98 ASV.dial :PCRlock @13:16:09.31 delay 5 seconds @13:16:14.32 Running ENTap Profile60C thermocycling @13:16:14.33 PCR.program Profile60C @13:16:14.43 Profile60C should complete in < 1:39:08 @13:16:14.44 PCR.run 2:08:55 @14:38:44.69 PCR.off @14:38:45.20 PCR for ENTap completed -- closed /var/log/esp/ENTap14mar0513h274ml.pcr @14:38:45.25 Cleaning PCR @14:38:45.26 AFV.select :carrierSolution @14:38:45.35 delay 2 seconds @14:38:47.36 AS.reconfigure ASconfig @14:38:47.40 AS.seek 0.500ml @14:38:57.82 delay 1 second @14:38:58.83 AFV.select :holdingCoil @14:38:58.92 ASV.dial :waste1 @14:38:59.40 ATV.dial :air3 @14:39:00.34 AS.reconfigure ASslow1 @14:39:00.37 AS.seek 0.510ml @14:39:02.68 delay 2 seconds @14:39:04.69 ATV.dial :bleach @14:39:05.17 AS.seek 0.810ml @14:40:06.60 delay 3 seconds @14:40:09.61 ATV.dial :air3 @14:40:09.93 delay 3 seconds @14:40:12.99 AS.seek 0.835ml @14:40:18.32 delay 3 seconds @14:40:21.33 ATV.dial :bleach @14:40:22.28 AS.seek 0.820ml @14:40:25.62 delay 3 seconds @14:40:28.63 ARV.dial :waste @14:40:29.23 delay 3 seconds @14:40:32.24 ATV.dial :ARV @14:40:32.97 AS.reconfigure ASconfig @14:40:33.01 AS.seek 0.470ml @14:40:53.68 AFV.select :carrierSolution @14:40:53.77 delay 2 seconds @14:40:55.78 AS.seek 1.000ml @14:41:26.96 delay 1 second @14:41:27.97 AFV.select :holdingCoil @14:41:28.06 delay 1 second @14:41:29.07 AS.reconfigure ASfast @14:41:29.11 AS.seek 0.000ml @14:41:56.82 AFV.select :carrierSolution @14:41:56.92 delay 2 seconds @14:41:58.93 AS.reconfigure ASconfig @14:41:58.96 AS.seek 1.000ml @14:42:57.52 delay 1 second @14:42:58.53 AFV.select :holdingCoil @14:42:58.62 delay 1 second @14:42:59.63 AS.reconfigure ASfast @14:42:59.67 AS.seek 0.000ml @14:43:27.39 AFV.select :carrierSolution @14:43:27.49 delay 2 seconds @14:43:29.50 AS.reconfigure ASconfig @14:43:29.53 AS.seek 1.000ml @14:44:28.10 delay 1 second @14:44:29.11 AFV.select :holdingCoil @14:44:29.20 delay 1 second @14:44:30.21 AS.reconfigure ASfast @14:44:30.24 AS.seek 0.000ml @14:44:57.98 ARV.dial between carrier and air1 @14:44:58.51 ATV.dial :air3 @14:44:59.45 AS.reconfigure ASslow1 @14:44:59.48 AS.seek 0.010ml @14:45:01.82 delay 2 seconds @14:45:03.83 ATV.dial :bleach @14:45:04.31 AS.seek 0.810ml @14:47:47.71 delay 3 seconds @14:47:50.72 ATV.dial :air3 @14:47:51.05 delay 3 seconds @14:47:54.11 AS.seek 0.835ml @14:47:59.44 delay 3 seconds @14:48:02.45 ATV.dial :bleach @14:48:02.85 AS.seek 0.820ml @14:48:06.16 delay 3 seconds @14:48:09.17 ATV.dial PCR @14:48:09.49 AS.reconfigure ASconfig @14:48:09.53 AS.seek empty @14:48:57.66 PCR.program :bleachProfile @14:48:57.72 PCR program should complete in < 2:45 @14:48:57.73 PCR.run 10:00 @14:51:58.94 PCR.off @14:51:58.97 AFV.select :carrierSolution @14:51:59.06 delay 2 seconds @14:52:01.07 AS.seek 1.000ml @14:52:59.74 delay 1 second @14:53:00.75 AFV.select :holdingCoil @14:53:00.99 delay 1 second @14:53:02.00 AS.reconfigure ASfast @14:53:02.04 AS.seek 0.000ml @14:53:29.74 delay 3 seconds @14:53:32.75 AFV.select :carrierSolution @14:53:32.84 delay 2 seconds @14:53:34.85 AS.reconfigure ASconfig @14:53:34.88 AS.seek 1.000ml @14:54:33.47 delay 1 second @14:54:34.48 AFV.select :holdingCoil @14:54:34.57 delay 1 second @14:54:35.58 AS.reconfigure ASfast @14:54:35.62 AS.seek 0.000ml @14:55:03.38 delay 3 seconds @14:55:06.39 AFV.select :carrierSolution @14:55:06.48 delay 2 seconds @14:55:08.49 AS.reconfigure ASconfig @14:55:08.52 AS.seek 1.000ml @14:56:07.11 delay 1 second @14:56:08.12 AFV.select :holdingCoil @14:56:08.21 delay 1 second @14:56:09.22 AS.reconfigure ASfast @14:56:09.25 AS.seek 0.000ml @14:56:36.95 delay 3 seconds @14:56:39.96 AFV.select :carrierSolution @14:56:40.05 delay 2 seconds @14:56:42.06 AS.reconfigure ASconfig @14:56:42.09 AS.seek 1.000ml @14:57:40.69 delay 1 second @14:57:41.70 AFV.select :holdingCoil @14:57:41.79 delay 1 second @14:57:42.80 AS.reconfigure ASfast @14:57:42.83 AS.seek 0.000ml @14:58:10.53 delay 3 seconds @14:58:13.54 AFV.select :carrierSolution @14:58:13.63 delay 2 seconds @14:58:15.64 AS.reconfigure ASconfig @14:58:15.67 AS.seek 1.000ml @14:59:14.26 delay 1 second @14:59:15.27 AFV.select :holdingCoil @14:59:15.36 delay 1 second @14:59:16.37 AS.reconfigure ASnorm @14:59:16.40 AS.seek 0.000ml @15:00:07.87 Setting up PCR for ENTem -- writing /var/log/esp/ENTem14mar0515h274ml.pcr @15:00:07.88 Building PCR Slug from ASV with MM2 mix, PP3 primer @15:00:07.89 Priming with carrier @15:00:07.90 ATV.dial ARV @15:00:08.42 ARV.dial :carrier @15:00:08.70 AS.seek 0.500ml @15:00:34.57 AFV.select :carrierSolution @15:00:34.66 delay 2 seconds @15:00:36.67 AS.reconfigure ASconfig @15:00:36.70 AS.seek 1.000ml @15:01:06.13 delay 1 second @15:01:07.14 AFV.select :holdingCoil @15:01:07.23 delay 1 second @15:01:08.24 ATV.dial :waste @15:01:09.52 AS.reconfigure ASnorm @15:01:09.55 AS.seek empty @15:02:01.05 AFV.select :carrierSolution @15:02:01.14 delay 2 seconds @15:02:03.15 AS.reconfigure ASconfig @15:02:03.18 AS.seek 0.500ml @15:02:32.66 delay 1 second @15:02:33.67 AFV.select :holdingCoil @15:02:33.76 Building Slug @15:02:33.77 ATV.dial :ARV @15:02:35.11 delay 2 seconds @15:02:37.12 ARV.dial :air1 @15:02:37.45 delay 2 seconds @15:02:40.03 AS.reconfigure ASslow2 @15:02:40.06 AS.seek 0.506ml @15:02:46.53 delay 2 seconds @15:02:48.54 ARV.dial :MM2 @15:02:49.77 delay 2 seconds @15:02:51.78 Adding MM2 @15:02:51.84 AS.seek 0.524ml @15:03:10.59 delay 25 seconds @15:03:35.60 ARV.dial :PP3 @15:03:36.02 Adding PP3 @15:03:36.03 delay 2 seconds @15:03:38.08 AS.seek 0.530ml @15:03:44.56 delay 10 seconds @15:03:54.57 ARV.dial :air2 @15:03:55.01 adding ARV air @15:03:55.03 delay 2 seconds @15:03:57.08 AS.seek 0.536ml @15:04:03.29 delay 5 seconds @15:04:08.30 ARV.dial :carrier @15:04:09.34 delay 7 seconds @15:04:16.40 AS.seek 0.594ml @15:05:15.71 delay 5 seconds @15:05:20.72 ATV.dial :ASV @15:05:21.04 delay 2 seconds @15:05:23.10 AS.seek 0.600ml @15:05:29.43 delay 4 seconds @15:05:33.44 ATV.dial ARV @15:05:33.76 delay 2 seconds @15:05:35.77 ARV.dial :carrier @15:05:35.78 delay 2 seconds @15:05:37.83 AS.seek 0.650ml @15:06:28.96 delay 4 seconds @15:06:32.97 ATV.dial PCR @15:06:33.59 ASV.dial :waste1 @15:06:33.60 ARV.dial :air1 @15:06:33.94 Positioning Slug by 0.324ml @15:06:33.99 AS.seek 0.326ml @15:12:04.47 delay 5 seconds @15:12:09.48 ATV.dial ARV @15:12:10.08 ASV.dial :PCRlock @15:12:10.58 delay 5 seconds @15:12:15.59 Running ENTem ProfileEM60C thermocycling @15:12:15.60 PCR.program ProfileEM60C @15:12:15.71 ProfileEM60C should complete in < 2:11:38 @15:12:15.72 PCR.run 2:49:32.5 @17:06:57.53 PCR.off @17:06:58.06 PCR for ENTem completed -- closed /var/log/esp/ENTem14mar0515h274ml.pcr @17:06:58.11 Cleaning PCR @17:06:58.12 AFV.select :carrierSolution @17:06:58.21 delay 2 seconds @17:07:00.22 AS.reconfigure ASconfig @17:07:00.25 AS.seek 0.500ml @17:07:10.71 delay 1 second @17:07:11.72 AFV.select :holdingCoil @17:07:11.81 ASV.dial :waste1 @17:07:12.30 ATV.dial :air3 @17:07:13.79 AS.reconfigure ASslow1 @17:07:13.82 AS.seek 0.510ml @17:07:16.11 delay 2 seconds @17:07:18.12 ATV.dial :bleach @17:07:18.51 AS.seek 0.810ml @17:08:19.89 delay 3 seconds @17:08:22.90 ATV.dial :air3 @17:08:23.24 delay 3 seconds @17:08:26.30 AS.seek 0.835ml @17:08:31.63 delay 3 seconds @17:08:34.64 ATV.dial :bleach @17:08:35.04 AS.seek 0.820ml @17:08:38.36 delay 3 seconds @17:08:41.37 ARV.dial :waste @17:08:41.86 delay 3 seconds @17:08:44.87 ATV.dial :ARV @17:08:45.62 AS.reconfigure ASconfig @17:08:45.65 AS.seek 0.470ml @17:09:06.31 AFV.select :carrierSolution @17:09:06.41 delay 2 seconds @17:09:08.42 AS.seek 1.000ml @17:09:39.63 delay 1 second @17:09:40.64 AFV.select :holdingCoil @17:09:40.73 delay 1 second @17:09:41.74 AS.reconfigure ASfast @17:09:41.77 AS.seek 0.000ml @17:10:09.48 AFV.select :carrierSolution @17:10:09.58 delay 2 seconds @17:10:11.59 AS.reconfigure ASconfig @17:10:11.62 AS.seek 1.000ml @17:11:10.22 delay 1 second @17:11:11.23 AFV.select :holdingCoil @17:11:11.32 delay 1 second @17:11:12.33 AS.reconfigure ASfast @17:11:12.36 AS.seek 0.000ml @17:11:40.08 AFV.select :carrierSolution @17:11:40.18 delay 2 seconds @17:11:42.19 AS.reconfigure ASconfig @17:11:42.22 AS.seek 1.000ml @17:12:40.84 delay 1 second @17:12:41.85 AFV.select :holdingCoil @17:12:41.94 delay 1 second @17:12:42.95 AS.reconfigure ASfast @17:12:42.98 AS.seek 0.000ml @17:13:10.71 ARV.dial between carrier and air1 @17:13:11.23 ATV.dial :air3 @17:13:12.19 AS.reconfigure ASslow1 @17:13:12.22 AS.seek 0.010ml @17:13:14.56 delay 2 seconds @17:13:16.57 ATV.dial :bleach @17:13:16.97 AS.seek 0.810ml @17:16:00.38 delay 3 seconds @17:16:03.39 ATV.dial :air3 @17:16:03.74 delay 3 seconds @17:16:06.80 AS.seek 0.835ml @17:16:12.13 delay 3 seconds @17:16:15.14 ATV.dial :bleach @17:16:15.54 AS.seek 0.820ml @17:16:18.86 delay 3 seconds @17:16:21.87 ATV.dial PCR @17:16:22.20 AS.reconfigure ASconfig @17:16:22.23 AS.seek empty @17:17:10.35 PCR.program :bleachProfile @17:17:10.41 PCR program should complete in < 2:45 @17:17:10.42 PCR.run 10:00 @17:20:11.64 PCR.off @17:20:11.67 AFV.select :carrierSolution @17:20:11.76 delay 2 seconds @17:20:13.77 AS.seek 1.000ml @17:21:12.44 delay 1 second @17:21:13.45 AFV.select :holdingCoil @17:21:13.54 delay 1 second @17:21:14.55 AS.reconfigure ASfast @17:21:14.58 AS.seek 0.000ml @17:21:42.30 delay 3 seconds @17:21:45.31 AFV.select :carrierSolution @17:21:45.40 delay 2 seconds @17:21:47.41 AS.reconfigure ASconfig @17:21:47.44 AS.seek 1.000ml @17:22:46.03 delay 1 second @17:22:47.04 AFV.select :holdingCoil @17:22:47.13 delay 1 second @17:22:48.14 AS.reconfigure ASfast @17:22:48.17 AS.seek 0.000ml @17:23:15.91 delay 3 seconds @17:23:18.92 AFV.select :carrierSolution @17:23:19.01 delay 2 seconds @17:23:21.02 AS.reconfigure ASconfig @17:23:21.05 AS.seek 1.000ml @17:24:19.62 delay 1 second @17:24:20.63 AFV.select :holdingCoil @17:24:20.72 delay 1 second @17:24:21.73 AS.reconfigure ASfast @17:24:21.76 AS.seek 0.000ml @17:24:49.48 delay 3 seconds @17:24:52.49 AFV.select :carrierSolution @17:24:52.58 delay 2 seconds @17:24:54.59 AS.reconfigure ASconfig @17:24:54.62 AS.seek 1.000ml @17:25:53.22 delay 1 second @17:25:54.23 AFV.select :holdingCoil @17:25:54.32 delay 1 second @17:25:55.33 AS.reconfigure ASfast @17:25:55.36 AS.seek 0.000ml @17:26:23.10 delay 3 seconds @17:26:26.11 AFV.select :carrierSolution @17:26:26.20 delay 2 seconds @17:26:28.21 AS.reconfigure ASconfig @17:26:28.24 AS.seek 1.000ml @17:27:26.84 delay 1 second @17:27:27.85 AFV.select :holdingCoil @17:27:27.94 delay 1 second @17:27:28.95 AS.reconfigure ASnorm @17:27:28.99 AS.seek 0.000ml @17:28:20.46 Setting up PCR for IPCap -- writing /var/log/esp/IPCap14mar0517h274ml.pcr @17:28:20.47 Building PCR Slug from ASV with MM1 mix, PP2 primer @17:28:20.48 Priming with carrier @17:28:20.49 ATV.dial ARV @17:28:21.01 ARV.dial :carrier @17:28:21.28 AS.seek 0.500ml @17:28:47.15 AFV.select :carrierSolution @17:28:47.24 delay 2 seconds @17:28:49.25 AS.reconfigure ASconfig @17:28:49.28 AS.seek 1.000ml @17:29:18.74 delay 1 second @17:29:19.75 AFV.select :holdingCoil @17:29:19.84 delay 1 second @17:29:20.85 ATV.dial :waste @17:29:22.78 AS.reconfigure ASnorm @17:29:22.81 AS.seek empty @17:30:14.28 AFV.select :carrierSolution @17:30:14.38 delay 2 seconds @17:30:16.39 AS.reconfigure ASconfig @17:30:16.43 AS.seek 0.500ml @17:30:45.92 delay 1 second @17:30:46.93 AFV.select :holdingCoil @17:30:47.02 Building Slug @17:30:47.03 ATV.dial :ARV @17:30:48.38 delay 2 seconds @17:30:50.39 ARV.dial :air1 @17:30:50.72 delay 2 seconds @17:30:52.78 AS.reconfigure ASslow2 @17:30:52.81 AS.seek 0.506ml @17:30:59.10 delay 2 seconds @17:31:01.11 ARV.dial :MM1 @17:31:01.69 delay 2 seconds @17:31:03.70 Adding MM1 @17:31:03.76 AS.seek 0.524ml @17:31:22.36 delay 25 seconds @17:31:47.37 ARV.dial :PP2 @17:31:47.70 Adding PP2 @17:31:47.71 delay 2 seconds @17:31:49.77 AS.seek 0.530ml @17:31:56.44 delay 10 seconds @17:32:06.45 ARV.dial :air2 @17:32:06.88 adding ARV air @17:32:06.89 delay 2 seconds @17:32:08.95 AS.seek 0.536ml @17:32:15.39 delay 5 seconds @17:32:20.40 ARV.dial :carrier @17:32:21.44 delay 7 seconds @17:32:28.50 AS.seek 0.594ml @17:33:27.79 delay 5 seconds @17:33:32.80 ATV.dial :ASV @17:33:33.12 delay 2 seconds @17:33:35.18 AS.seek 0.600ml @17:33:41.70 delay 4 seconds @17:33:45.71 ATV.dial ARV @17:33:46.04 delay 2 seconds @17:33:48.05 ARV.dial :carrier @17:33:48.06 delay 2 seconds @17:33:50.11 AS.seek 0.650ml @17:34:41.36 delay 4 seconds @17:34:45.37 ATV.dial PCR @17:34:46.00 ASV.dial :waste1 @17:34:46.01 ARV.dial :air1 @17:34:46.47 Positioning Slug by 0.324ml @17:34:46.52 AS.seek 0.326ml @17:40:17.32 delay 5 seconds @17:40:22.33 ATV.dial ARV @17:40:22.84 ASV.dial :PCRlock @17:40:23.32 delay 5 seconds @17:40:28.33 Running IPCap Profile59C thermocycling @17:40:28.34 PCR.program Profile59C @17:40:28.44 Profile59C should complete in < 1:39:08 @17:40:28.45 PCR.run 2:08:55 @19:02:58.09 PCR.off @19:02:58.64 PCR for IPCap completed -- closed /var/log/esp/IPCap14mar0517h274ml.pcr @19:02:58.69 Cleaning PCR @19:02:58.70 AFV.select :carrierSolution @19:02:58.79 delay 2 seconds @19:03:00.80 AS.reconfigure ASconfig @19:03:00.83 AS.seek 0.500ml @19:03:11.25 delay 1 second @19:03:12.26 AFV.select :holdingCoil @19:03:12.35 ASV.dial :waste1 @19:03:12.83 ATV.dial :air3 @19:03:13.77 AS.reconfigure ASslow1 @19:03:13.80 AS.seek 0.510ml @19:03:16.11 delay 2 seconds @19:03:18.12 ATV.dial :bleach @19:03:18.52 AS.seek 0.810ml @19:04:20.00 delay 3 seconds @19:04:23.01 ATV.dial :air3 @19:04:23.36 delay 3 seconds @19:04:26.42 AS.seek 0.835ml @19:04:31.76 delay 3 seconds @19:04:34.77 ATV.dial :bleach @19:04:35.25 AS.seek 0.820ml @19:04:38.58 delay 3 seconds @19:04:41.59 ARV.dial :waste @19:04:42.09 delay 3 seconds @19:04:45.10 ATV.dial :ARV @19:04:45.83 AS.reconfigure ASconfig @19:04:45.87 AS.seek 0.470ml @19:05:06.54 AFV.select :carrierSolution @19:05:06.63 delay 2 seconds @19:05:08.64 AS.seek 1.000ml @19:05:39.84 delay 1 second @19:05:40.85 AFV.select :holdingCoil @19:05:40.94 delay 1 second @19:05:41.95 AS.reconfigure ASfast @19:05:41.98 AS.seek 0.000ml @19:06:09.71 AFV.select :carrierSolution @19:06:09.81 delay 2 seconds @19:06:11.82 AS.reconfigure ASconfig @19:06:11.85 AS.seek 1.000ml @19:07:10.46 delay 1 second @19:07:11.47 AFV.select :holdingCoil @19:07:11.56 delay 1 second @19:07:12.57 AS.reconfigure ASfast @19:07:12.60 AS.seek 0.000ml @19:07:40.30 AFV.select :carrierSolution @19:07:40.40 delay 2 seconds @19:07:42.41 AS.reconfigure ASconfig @19:07:42.44 AS.seek 1.000ml @19:08:41.02 delay 1 second @19:08:42.03 AFV.select :holdingCoil @19:08:42.12 delay 1 second @19:08:43.13 AS.reconfigure ASfast @19:08:43.16 AS.seek 0.000ml @19:09:10.91 ARV.dial between carrier and air1 @19:09:11.43 ATV.dial :air3 @19:09:12.38 AS.reconfigure ASslow1 @19:09:12.41 AS.seek 0.010ml @19:09:14.75 delay 2 seconds @19:09:16.76 ATV.dial :bleach @19:09:17.15 AS.seek 0.810ml @19:12:00.53 delay 3 seconds @19:12:03.54 ATV.dial :air3 @19:12:03.89 delay 3 seconds @19:12:06.95 AS.seek 0.835ml @19:12:12.30 delay 3 seconds @19:12:15.31 ATV.dial :bleach @19:12:15.70 AS.seek 0.820ml @19:12:19.01 delay 3 seconds @19:12:22.02 ATV.dial PCR @19:12:22.34 AS.reconfigure ASconfig @19:12:22.37 AS.seek empty @19:13:10.50 PCR.program :bleachProfile @19:13:10.55 PCR program should complete in < 2:45 @19:13:10.56 PCR.run 10:00 @19:16:11.77 PCR.off @19:16:11.80 AFV.select :carrierSolution @19:16:11.89 delay 2 seconds @19:16:13.90 AS.seek 1.000ml @19:17:12.51 delay 1 second @19:17:13.52 AFV.select :holdingCoil @19:17:13.61 delay 1 second @19:17:14.62 AS.reconfigure ASfast @19:17:14.65 AS.seek 0.000ml @19:17:42.39 delay 3 seconds @19:17:45.40 AFV.select :carrierSolution @19:17:45.49 delay 2 seconds @19:17:47.50 AS.reconfigure ASconfig @19:17:47.53 AS.seek 1.000ml @19:18:46.12 delay 1 second @19:18:47.13 AFV.select :holdingCoil @19:18:47.22 delay 1 second @19:18:48.23 AS.reconfigure ASfast @19:18:48.26 AS.seek 0.000ml @19:19:15.96 delay 3 seconds @19:19:18.97 AFV.select :carrierSolution @19:19:19.06 delay 2 seconds @19:19:21.07 AS.reconfigure ASconfig @19:19:21.10 AS.seek 1.000ml @19:20:19.70 delay 1 second @19:20:20.71 AFV.select :holdingCoil @19:20:20.80 delay 1 second @19:20:21.81 AS.reconfigure ASfast @19:20:21.84 AS.seek 0.000ml @19:20:49.54 delay 3 seconds @19:20:52.55 AFV.select :carrierSolution @19:20:52.64 delay 2 seconds @19:20:54.65 AS.reconfigure ASconfig @19:20:54.68 AS.seek 1.000ml @19:21:53.29 delay 1 second @19:21:54.30 AFV.select :holdingCoil @19:21:54.39 delay 1 second @19:21:55.40 AS.reconfigure ASfast @19:21:55.43 AS.seek 0.000ml @19:22:23.18 delay 3 seconds @19:22:26.19 AFV.select :carrierSolution @19:22:26.28 delay 2 seconds @19:22:28.29 AS.reconfigure ASconfig @19:22:28.32 AS.seek 1.000ml @19:23:26.93 delay 1 second @19:23:27.94 AFV.select :holdingCoil @19:23:28.03 delay 1 second @19:23:29.04 AS.reconfigure ASnorm @19:23:29.58 AS.seek 0.000ml @19:24:21.03 Setting up PCR for IPCem -- writing /var/log/esp/IPCem14mar0519h274ml.pcr @19:24:21.04 Building PCR Slug from ASV with MM3 mix, PP5 primer @19:24:21.05 Priming with carrier @19:24:21.06 ATV.dial ARV @19:24:21.56 ARV.dial :carrier @19:24:21.83 AS.seek 0.500ml @19:24:47.74 AFV.select :carrierSolution @19:24:47.85 delay 2 seconds @19:24:49.86 AS.reconfigure ASconfig @19:24:49.90 AS.seek 1.000ml @19:25:19.33 delay 1 second @19:25:20.34 AFV.select :holdingCoil @19:25:20.44 delay 1 second @19:25:21.45 ATV.dial :waste @19:25:22.73 AS.reconfigure ASnorm @19:25:22.76 AS.seek empty @19:26:14.23 AFV.select :carrierSolution @19:26:14.33 delay 2 seconds @19:26:16.34 AS.reconfigure ASconfig @19:26:16.37 AS.seek 0.500ml @19:26:45.86 delay 1 second @19:26:46.87 AFV.select :holdingCoil @19:26:46.96 Building Slug @19:26:46.97 ATV.dial :ARV @19:26:48.31 delay 2 seconds @19:26:50.32 ARV.dial :air1 @19:26:50.65 delay 2 seconds @19:26:52.71 AS.reconfigure ASslow2 @19:26:52.74 AS.seek 0.506ml @19:26:59.09 delay 2 seconds @19:27:01.10 ARV.dial :MM3 @19:27:01.97 delay 2 seconds @19:27:03.98 Adding MM3 @19:27:04.03 AS.seek 0.524ml @19:27:22.61 delay 25 seconds @19:27:47.62 ARV.dial :PP5 @19:27:47.95 Adding PP5 @19:27:47.97 delay 2 seconds @19:27:50.02 AS.seek 0.530ml @19:27:56.32 delay 10 seconds @19:28:06.33 ARV.dial :air3 @19:28:06.64 adding ARV air @19:28:06.65 delay 2 seconds @19:28:08.71 AS.seek 0.536ml @19:28:14.95 delay 5 seconds @19:28:19.96 ARV.dial :carrier @19:28:20.99 delay 7 seconds @19:28:28.05 AS.seek 0.594ml @19:29:27.32 delay 5 seconds @19:29:32.33 ATV.dial :ASV @19:29:32.66 delay 2 seconds @19:29:34.72 AS.seek 0.600ml @19:29:41.05 delay 4 seconds @19:29:45.06 ATV.dial ARV @19:29:45.38 delay 2 seconds @19:29:47.39 ARV.dial :carrier @19:29:47.40 delay 2 seconds @19:29:49.45 AS.seek 0.650ml @19:30:40.69 delay 4 seconds @19:30:44.70 ATV.dial PCR @19:30:45.21 ASV.dial :waste1 @19:30:45.22 ARV.dial :air1 @19:30:45.56 Positioning Slug by 0.324ml @19:30:45.61 AS.seek 0.326ml @19:36:16.35 delay 5 seconds @19:36:21.36 ATV.dial ARV @19:36:21.90 ASV.dial :PCRlock @19:36:22.40 delay 5 seconds @19:36:27.41 Running IPCem ProfileEM59C thermocycling @19:36:27.42 PCR.program ProfileEM59C @19:36:27.53 ProfileEM59C should complete in < 2:11:38 @19:36:27.54 PCR.run 2:49:32.5 @21:31:38.14 PCR.off @21:31:38.68 PCR for IPCem completed -- closed /var/log/esp/IPCem14mar0519h274ml.pcr @21:31:38.69 Full MFB Decontamination @21:31:38.70 Decon extraction column and decon AIV @21:31:38.71 SPE.hold 2:46:40,55.0C @21:31:38.73 ASV.dial :SPEdelivery @21:31:39.68 delay 20 seconds @21:31:59.69 AFV.select :carrierSolution @21:31:59.78 delay 2 seconds @21:32:01.79 AS.reconfigure ASconfig @21:32:01.82 AS.seek 0.500ml @21:32:12.23 delay 1 second @21:32:13.24 AFV.select :holdingCoil @21:32:13.33 ATV.dial :air1 @21:32:14.85 AS.reconfigure ASslow1 @21:32:14.88 AS.seek 0.520ml @21:32:19.24 ATV.dial :ASV @21:32:20.52 ASV.dial :SPErecovery @21:32:21.23 AS.seek 0.620ml @21:32:41.88 ATV.dial :waste @21:32:43.36 AS.reconfigure ASnorm @21:32:43.39 AS.seek empty @21:33:15.44 AFV.select :holdingCoil @21:33:15.45 ATV.dial :waste @21:33:15.46 AS.reconfigure ASconfig @21:33:15.49 AS.seek 0.000ml @21:33:15.79 AFV.select :carrierSolution @21:33:15.90 delay 2 seconds @21:33:17.91 AS.seek 0.500ml @21:33:47.33 delay 1 second @21:33:48.34 AFV.select :holdingCoil @21:33:48.43 ATV.dial :air3 @21:33:49.00 AS.reconfigure ASslow1 @21:33:49.03 AS.seek 0.520ml @21:33:53.36 ATV.dial :air3 @21:33:53.42 AS.seek 0.530ml @21:33:55.70 delay 2 seconds @21:33:57.71 ATV.dial :bleach @21:33:58.08 AS.seek 0.710ml @21:34:35.01 delay 3 seconds @21:34:38.02 ATV.dial :air3 @21:34:38.45 delay 3 seconds @21:34:41.51 AS.seek 0.735ml @21:34:46.84 delay 3 seconds @21:34:49.85 ATV.dial :bleach @21:34:50.23 AS.seek 0.720ml @21:34:53.54 delay 3 seconds @21:34:56.55 ATV.dial ASV @21:34:57.53 ASV.dial :SPErecovery @21:34:57.59 AS.seek 0.365ml @21:36:10.21 ATV.dial :waste @21:36:11.78 AS.reconfigure ASnorm @21:36:11.81 AS.seek empty @21:36:30.77 ATV.dial :air3 @21:36:31.34 AS.reconfigure ASslow1 @21:36:31.37 AS.seek 0.009ml @21:36:33.68 delay 2 seconds @21:36:35.69 ATV.dial :bleach @21:36:36.07 AS.seek 0.579ml @21:38:32.52 delay 3 seconds @21:38:35.53 ATV.dial :air3 @21:38:35.86 delay 3 seconds @21:38:38.92 AS.seek 0.604ml @21:38:44.29 delay 3 seconds @21:38:47.30 ATV.dial :bleach @21:38:47.69 AS.seek 0.589ml @21:38:50.99 delay 3 seconds @21:38:54.00 ATV.dial :ASV @21:38:54.89 ASV.dial :wasteR @21:38:55.65 AS.seek 0.000ml @21:40:56.02 delay 1:00 @21:41:56.03 ATV.dial ASV @21:41:56.04 AFV.select :carrierSolution @21:41:56.13 delay 2 seconds @21:41:58.14 AS.reconfigure ASconfig @21:41:58.17 AS.seek 1.000ml @21:42:56.78 delay 1 second @21:42:57.79 AFV.select :holdingCoil @21:42:57.88 delay 1 second @21:42:58.89 ASV.dial :wasteR @21:42:58.90 AS.reconfigure ASslow1 @21:42:58.93 AS.seek 0.000ml @21:46:23.13 delay 10 seconds @21:46:33.14 ATV.dial :air3 @21:46:34.27 AS.seek 0.010ml @21:46:36.61 delay 2 seconds @21:46:38.62 ATV.dial :bleach @21:46:39.10 AS.seek 0.580ml @21:48:35.61 delay 3 seconds @21:48:38.62 ATV.dial :air3 @21:48:38.94 delay 3 seconds @21:48:42.00 AS.seek 0.605ml @21:48:47.42 delay 3 seconds @21:48:50.43 ATV.dial :bleach @21:48:50.82 AS.seek 0.590ml @21:48:54.09 delay 3 seconds @21:48:57.10 ATV.dial ASV @21:48:57.97 ASV.dial :SPEdelivery @21:48:58.45 AS.seek 0.000ml @21:50:59.60 delay 1:00 @21:51:59.61 AFV.select :carrierSolution @21:51:59.71 delay 2 seconds @21:52:01.72 AS.reconfigure ASconfig @21:52:01.76 AS.seek 1.000ml @21:53:00.39 delay 1 second @21:53:01.40 AFV.select :holdingCoil @21:53:01.50 delay 1 second @21:53:02.51 ATV.dial ASV @21:53:02.52 ASV.dial :SPEdelivery @21:53:02.53 AS.reconfigure ASslow1 @21:53:02.56 AS.seek 0.000ml @21:56:26.84 delay 10 seconds @21:56:36.85 ATV.dial :air2 @21:56:37.58 AS.reconfigure ASslow0 @21:56:37.61 AS.seek 0.800ml @21:57:59.49 delay 3 seconds @21:58:02.50 ATV.dial :ASV @21:58:03.21 ASV.dial :SPEdelivery @21:58:03.23 AS.reconfigure ASslow1 @21:58:03.26 AS.seek 0.030ml @22:00:40.55 delay 20 seconds @22:01:00.56 ATV.dial :waste,via => air3 @22:01:02.01 AS.reconfigure ASconfig @22:01:02.04 AS.seek empty @22:01:04.14 SPE.coast @22:01:04.22 Removing Bleach from Mixing Coil @22:01:04.23 AFV.select :carrierSolution @22:01:04.32 delay 2 seconds @22:01:06.33 AS.seek 0.500ml @22:01:35.80 delay 1 second @22:01:36.81 AFV.select :holdingCoil @22:01:36.90 ATV.dial :ASV @22:01:38.33 ASV.dial :SPErecovery @22:01:39.30 AS.reconfigure ASslow1 @22:01:39.33 AS.seek 0.730ml @22:02:26.52 ASV.dial :wasteD @22:02:27.18 AS.reconfigure ASnorm @22:02:27.21 AS.seek 0.000ml @22:03:04.83 AFV.select :carrierSolution @22:03:04.93 delay 2 seconds @22:03:06.94 AS.reconfigure ASconfig @22:03:06.98 AS.seek 0.500ml @22:03:36.41 delay 1 second @22:03:37.42 AFV.select :holdingCoil @22:03:37.51 ATV.dial ASV @22:03:37.52 ASV.dial :SPErecovery @22:03:38.22 AS.reconfigure ASslow0 @22:03:38.25 AS.seek 0.300ml @22:03:58.94 ATV.dial :air2 @22:03:59.80 AS.reconfigure ASslow1 @22:03:59.84 AS.seek 0.320ml @22:04:04.19 ATV.dial ASV @22:04:04.95 AS.reconfigure ASslow0 @22:04:04.98 AS.seek 0.570ml @22:04:30.78 ASV.dial :wasteD @22:04:31.44 AS.reconfigure ASnorm @22:04:31.47 AS.seek 0.000ml @22:05:00.93 AFV.select :carrierSolution @22:05:01.03 delay 2 seconds @22:05:03.04 AS.reconfigure ASconfig @22:05:03.07 AS.seek 0.500ml @22:05:32.51 delay 1 second @22:05:33.52 AFV.select :holdingCoil @22:05:33.61 ATV.dial ASV @22:05:33.62 ASV.dial :SPErecovery @22:05:34.32 AS.reconfigure ASslow0 @22:05:34.36 AS.seek 0.300ml @22:05:55.06 ATV.dial :air2 @22:05:55.83 AS.reconfigure ASslow1 @22:05:55.86 AS.seek 0.320ml @22:06:00.28 ATV.dial ASV @22:06:01.06 AS.reconfigure ASslow0 @22:06:01.09 AS.seek 0.570ml @22:06:26.85 ASV.dial :wasteD @22:06:27.78 AS.reconfigure ASnorm @22:06:27.81 AS.seek 0.000ml @22:06:57.26 ATV.dial :air2 @22:06:57.99 AS.reconfigure ASslow0 @22:06:58.02 AS.seek 0.300ml @22:07:28.99 ATV.dial :ASV @22:07:29.70 ASV.dial :wasteD @22:07:29.72 AS.reconfigure ASconfig @22:07:29.75 AS.seek 0.050ml @22:07:44.61 ATV.dial :waste @22:07:46.06 AS.seek 0.000ml @22:07:49.27 ASV.dial :SPEdelivery @22:07:50.39 deconLysateINT @22:07:50.40 ATV.dial :air3 @22:07:50.97 AS.reconfigure ASslow1 @22:07:51.00 AS.seek 0.010ml @22:07:53.36 delay 2 seconds @22:07:55.37 ATV.dial :bleach @22:07:55.74 AS.seek 0.960ml @22:11:09.81 delay 3 seconds @22:11:12.82 ATV.dial :air3 @22:11:13.17 delay 3 seconds @22:11:16.23 AS.seek 0.985ml @22:11:21.53 delay 3 seconds @22:11:24.54 ATV.dial :bleach @22:11:25.03 AS.seek 0.970ml @22:11:28.37 delay 3 seconds @22:11:31.38 ATV.dial :AIV @22:11:32.46 AIV.dial :coilToMFB @22:11:32.47 AWV.select :waste @22:11:32.48 AFV.select :carrierSolution @22:11:32.57 delay 2 seconds @22:11:34.58 AS.reconfigure ASconfig @22:11:34.61 AS.seek 1.000ml @22:11:36.70 delay 1 second @22:11:37.71 AFV.select :holdingCoil @22:11:37.80 delay 1 second @22:11:38.81 AS.reconfigure ASslow0 @22:11:38.84 AS.seek empty @22:13:21.27 AFV.select :carrierSolution @22:13:21.36 delay 2 seconds @22:13:23.37 AS.reconfigure ASconfig @22:13:23.40 AS.seek 1.000ml @22:14:22.07 delay 1 second @22:14:23.08 AFV.select :holdingCoil @22:14:23.17 delay 1 second @22:14:24.18 AS.reconfigure ASslow0 @22:14:24.21 AS.seek empty @22:16:06.63 AFV.select :carrierSolution @22:16:06.72 delay 2 seconds @22:16:08.73 AS.reconfigure ASconfig @22:16:08.76 AS.seek 1.000ml @22:17:07.94 delay 1 second @22:17:08.95 AFV.select :holdingCoil @22:17:09.04 delay 1 second @22:17:10.05 AS.reconfigure ASslow0 @22:17:10.08 AS.seek empty @22:18:52.48 ATV.dial :air2 @22:18:53.16 AS.reconfigure ASslow1 @22:18:53.19 AS.seek 0.009ml @22:18:55.53 AS.reconfigure ASslow0 @22:18:55.57 AS.seek 1.000ml @22:20:36.90 ATV.dial AIV @22:20:37.42 AS.seek 0.020ml @22:22:17.68 ATV.dial :waste @22:22:18.97 AS.seek 0.000ml @22:22:21.30 AWV.select :air @22:22:21.44 Cleaning PCR @22:22:21.45 AFV.select :carrierSolution @22:22:21.54 delay 2 seconds @22:22:23.55 AS.reconfigure ASconfig @22:22:23.58 AS.seek 0.500ml @22:22:53.00 delay 1 second @22:22:54.01 AFV.select :holdingCoil @22:22:54.10 ASV.dial :waste1 @22:22:54.58 ATV.dial :air3 @22:22:55.15 AS.reconfigure ASslow1 @22:22:55.18 AS.seek 0.510ml @22:22:57.50 delay 2 seconds @22:22:59.51 ATV.dial :bleach @22:22:59.88 AS.seek 0.810ml @22:24:01.30 delay 3 seconds @22:24:04.31 ATV.dial :air3 @22:24:04.66 delay 3 seconds @22:24:07.72 AS.seek 0.835ml @22:24:13.05 delay 3 seconds @22:24:16.06 ATV.dial :bleach @22:24:16.46 AS.seek 0.820ml @22:24:19.77 delay 3 seconds @22:24:22.78 ARV.dial :waste @22:24:23.28 delay 3 seconds @22:24:26.29 ATV.dial :ARV @22:24:27.04 AS.reconfigure ASconfig @22:24:27.07 AS.seek 0.470ml @22:24:47.78 AFV.select :carrierSolution @22:24:47.87 delay 2 seconds @22:24:49.88 AS.seek 1.000ml @22:25:21.10 delay 1 second @22:25:22.11 AFV.select :holdingCoil @22:25:22.20 delay 1 second @22:25:23.21 AS.reconfigure ASfast @22:25:23.24 AS.seek 0.000ml @22:25:50.95 AFV.select :carrierSolution @22:25:51.05 delay 2 seconds @22:25:53.06 AS.reconfigure ASconfig @22:25:53.09 AS.seek 1.000ml @22:26:51.62 delay 1 second @22:26:52.63 AFV.select :holdingCoil @22:26:52.72 delay 1 second @22:26:53.73 AS.reconfigure ASfast @22:26:53.76 AS.seek 0.000ml @22:27:21.49 AFV.select :carrierSolution @22:27:21.59 delay 2 seconds @22:27:23.60 AS.reconfigure ASconfig @22:27:23.63 AS.seek 1.000ml @22:28:22.22 delay 1 second @22:28:23.23 AFV.select :holdingCoil @22:28:23.32 delay 1 second @22:28:24.33 AS.reconfigure ASfast @22:28:24.36 AS.seek 0.000ml @22:28:52.10 ARV.dial between carrier and air1 @22:28:52.52 ATV.dial :air3 @22:28:53.49 AS.reconfigure ASslow1 @22:28:53.52 AS.seek 0.010ml @22:28:55.85 delay 2 seconds @22:28:57.86 ATV.dial :bleach @22:28:58.26 AS.seek 0.810ml @22:31:41.65 delay 3 seconds @22:31:44.66 ATV.dial :air3 @22:31:45.00 delay 3 seconds @22:31:48.06 AS.seek 0.835ml @22:31:53.43 delay 3 seconds @22:31:56.44 ATV.dial :bleach @22:31:56.83 AS.seek 0.820ml @22:32:00.14 delay 3 seconds @22:32:03.15 ATV.dial PCR @22:32:03.49 AS.reconfigure ASconfig @22:32:03.52 AS.seek empty @22:32:51.65 PCR.program :bleachProfile @22:32:51.71 PCR program should complete in < 2:45 @22:32:51.72 PCR.run 10:00 @22:35:58.97 PCR.off @22:35:59.00 AFV.select :carrierSolution @22:35:59.09 delay 2 seconds @22:36:01.10 AS.seek 1.000ml @22:36:59.83 delay 1 second @22:37:00.84 AFV.select :holdingCoil @22:37:00.93 delay 1 second @22:37:01.94 AS.reconfigure ASfast @22:37:01.97 AS.seek 0.000ml @22:37:29.69 delay 3 seconds @22:37:32.70 AFV.select :carrierSolution @22:37:32.79 delay 2 seconds @22:37:34.80 AS.reconfigure ASconfig @22:37:34.83 AS.seek 1.000ml @22:38:33.41 delay 1 second @22:38:34.42 AFV.select :holdingCoil @22:38:34.51 delay 1 second @22:38:35.52 AS.reconfigure ASfast @22:38:35.55 AS.seek 0.000ml @22:39:03.28 delay 3 seconds @22:39:06.29 AFV.select :carrierSolution @22:39:06.38 delay 2 seconds @22:39:08.39 AS.reconfigure ASconfig @22:39:08.42 AS.seek 1.000ml @22:40:07.03 delay 1 second @22:40:08.04 AFV.select :holdingCoil @22:40:08.13 delay 1 second @22:40:09.14 AS.reconfigure ASfast @22:40:09.17 AS.seek 0.000ml @22:40:36.89 delay 3 seconds @22:40:39.90 AFV.select :carrierSolution @22:40:39.99 delay 2 seconds @22:40:42.53 AS.reconfigure ASconfig @22:40:42.56 AS.seek 1.000ml @22:41:41.18 delay 1 second @22:41:42.19 AFV.select :holdingCoil @22:41:42.28 delay 1 second @22:41:43.29 AS.reconfigure ASfast @22:41:43.32 AS.seek 0.000ml @22:42:11.07 delay 3 seconds @22:42:14.08 AFV.select :carrierSolution @22:42:14.17 delay 2 seconds @22:42:16.18 AS.reconfigure ASconfig @22:42:16.21 AS.seek 1.000ml @22:43:14.80 delay 1 second @22:43:15.81 AFV.select :holdingCoil @22:43:15.90 delay 1 second @22:43:16.91 AS.reconfigure ASnorm @22:43:16.95 AS.seek 0.000ml @22:44:08.41 AFV.select :carrierSolution @22:44:08.50 delay 2 seconds @22:44:10.51 AS.reconfigure ASconfig @22:44:10.54 AS.seek 1.000ml @22:45:09.12 delay 1 second @22:45:10.13 AFV.select :holdingCoil @22:45:10.22 delay 1 second @22:45:11.23 ATV.dial :waste @22:45:12.15 AS.reconfigure ASnorm @22:45:12.18 AS.seek 0.500ml @22:45:38.12 ATV.dial :waste2 @22:45:39.32 AS.seek 0.000ml @22:46:05.26
Cleaning PCR AFV.select :carrierSolution @22:46:05.35 delay 2 seconds @22:46:07.36 AS.reconfigure ASconfig @22:46:07.39 AS.seek 0.500ml @22:46:36.82 delay 1 second @22:46:37.83 AFV.select :holdingCoil @22:46:37.92 ASV.dial :waste1 @22:46:37.93 ATV.dial :air3 @22:46:39.44 AS.reconfigure ASslow1 @22:46:39.47 AS.seek 0.510ml @22:46:41.81 delay 2 seconds @22:46:43.82 ATV.dial :bleach @22:46:44.20 AS.seek 0.810ml @22:47:45.68 delay 3 seconds @22:47:48.69 ATV.dial :air3 @22:47:49.04 delay 3 seconds @22:47:52.10 AS.seek 0.835ml @22:47:57.41 delay 3 seconds @22:48:00.42 ATV.dial :bleach @22:48:00.81 AS.seek 0.820ml @22:48:04.13 delay 3 seconds @22:48:07.14 ARV.dial :waste @22:48:07.54 delay 3 seconds @22:48:10.55 ATV.dial :ARV @22:48:11.38 AS.reconfigure ASconfig @22:48:11.42 AS.seek 0.470ml @22:48:32.10 AFV.select :carrierSolution @22:48:32.19 delay 2 seconds @22:48:34.20 AS.seek 1.000ml @22:49:05.43 delay 1 second @22:49:06.44 AFV.select :holdingCoil @22:49:06.53 delay 1 second @22:49:07.54 AS.reconfigure ASfast @22:49:07.58 AS.seek 0.000ml @22:49:35.32 AFV.select :carrierSolution @22:49:35.41 delay 2 seconds @22:49:37.42 AS.reconfigure ASconfig @22:49:37.45 AS.seek 1.000ml @22:50:36.04 delay 1 second @22:50:37.05 AFV.select :holdingCoil @22:50:37.14 delay 1 second @22:50:38.15 AS.reconfigure ASfast @22:50:38.18 AS.seek 0.000ml @22:51:05.91 AFV.select :carrierSolution @22:51:06.00 delay 2 seconds @22:51:08.01 AS.reconfigure ASconfig @22:51:08.04 AS.seek 1.000ml @22:52:06.64 delay 1 second @22:52:07.65 AFV.select :holdingCoil @22:52:07.74 delay 1 second @22:52:08.75 AS.reconfigure ASfast @22:52:08.78 AS.seek 0.000ml @22:52:36.50 ARV.dial between carrier and air1 @22:52:36.91 ATV.dial :air3 @22:52:37.85 AS.reconfigure ASslow1 @22:52:37.89 AS.seek 0.010ml @22:52:40.23 delay 2 seconds @22:52:42.24 ATV.dial :bleach @22:52:42.72 AS.seek 0.810ml @22:55:26.13 delay 3 seconds @22:55:29.14 ATV.dial :air3 @22:55:29.46 delay 3 seconds @22:55:32.52 AS.seek 0.835ml @22:55:37.88 delay 3 seconds @22:55:40.89 ATV.dial :bleach @22:55:41.28 AS.seek 0.820ml @22:55:44.59 delay 3 seconds @22:55:47.60 ATV.dial PCR @22:55:47.93 AS.reconfigure ASconfig @22:55:47.96 AS.seek empty @22:56:36.09 PCR.program :bleachProfile @22:56:36.15 PCR program should complete in < 2:45 @22:56:36.16 PCR.run 10:00 @22:59:40.40 PCR.off @22:59:40.43 AFV.select :carrierSolution @22:59:40.52 delay 2 seconds @22:59:42.53 AS.seek 1.000ml @23:00:41.20 delay 1 second @23:00:42.21 AFV.select :holdingCoil @23:00:42.30 delay 1 second @23:00:43.31 AS.reconfigure ASfast @23:00:43.34 AS.seek 0.000ml @23:01:11.06 delay 3 seconds @23:01:14.07 AFV.select :carrierSolution @23:01:14.16 delay 2 seconds @23:01:16.17 AS.reconfigure ASconfig @23:01:16.20 AS.seek 1.000ml @23:02:14.77 delay 1 second @23:02:15.78 AFV.select :holdingCoil @23:02:15.87 delay 1 second @23:02:16.88 AS.reconfigure ASfast @23:02:16.91 AS.seek 0.000ml @23:02:44.65 delay 3 seconds @23:02:47.67 AFV.select :carrierSolution @23:02:47.76 delay 2 seconds @23:02:49.77 AS.reconfigure ASconfig @23:02:49.80 AS.seek 1.000ml @23:03:48.86 delay 1 second @23:03:49.88 AFV.select :holdingCoil @23:03:49.97 delay 1 second @23:03:50.98 AS.reconfigure ASfast @23:03:51.02 AS.seek 0.000ml @23:04:18.75 delay 3 seconds @23:04:21.76 AFV.select :carrierSolution @23:04:21.85 delay 2 seconds @23:04:23.86 AS.reconfigure ASconfig @23:04:23.90 AS.seek 1.000ml @23:05:22.52 delay 1 second @23:05:23.54 AFV.select :holdingCoil @23:05:23.63 delay 1 second @23:05:24.64 AS.reconfigure ASfast @23:05:24.67 AS.seek 0.000ml @23:05:52.42 delay 3 seconds @23:05:55.43 AFV.select :carrierSolution @23:05:55.52 delay 2 seconds @23:05:57.53 AS.reconfigure ASconfig @23:05:57.57 AS.seek 1.000ml @23:06:56.19 delay 1 second @23:06:57.20 AFV.select :holdingCoil @23:06:57.29 delay 1 second @23:06:58.30 AS.reconfigure ASnorm @23:06:58.33 AS.seek 0.000ml @23:07:49.78 Completed {wqPCR} begun 08:30:40 @23:07:49.80 AS.stop @23:07:49.82
Asleep til 8AM 3/6/14 @23:07:49.86 Sleeping until 08:00PST06-Mar-14 @23:07:49.88 GoodNews.email "Then, in tube 2: wqPCR\n/home/esp/esp2local/mission/14mar05sccwrpDeploy.rb:59",Subject => "Asleep until 08:00PST06-Mar-14" @23:07:51.49 CC.stop @23:07:51.52 CS.stop @23:07:51.55 PC.stop @23:07:51.58 PS.stop @23:07:51.61 Forearm.stop @23:07:51.64 Elbow.stop @23:07:51.67 SS.stop @23:07:51.74 Sleepy.power :raw,:OFF @23:07:51.75 Sleepy.power :dwarf,:OFF Shutdown @01:25:40.06PST06-Mar-14 Session Begun @01:25:40.08 -> Sleepy.cycleModemPower! 9 @01:25:40.11 Session Ended @08:00:00.34
Powering up Dwarves @08:00:05.49 Sleepy.power :raw,:ON @08:00:05.96 SPE.configure SPEconfig @08:00:05.98 AS.configure ASconfig @08:00:06.01 CC.configure CCconfig @08:00:06.04 CS.configure CSconfig @08:00:06.07 PC.configure PCconfig @08:00:06.10 PS.configure PSconfig @08:00:06.13 Forearm.configure LooseForearm @08:00:06.16 Elbow.configure LooseElbow @08:00:06.19 SC.configure SCloose @08:00:06.22 SE.configure SEconfig @08:00:06.25 SS.configure SSconfig @08:00:06.28 CH.configure CHconfig @08:00:06.30 PH.configure PHconfig @08:00:06.33 Valve.reset @08:00:06.99 PCR.off @08:00:07.02 PCR.pidtemp= 50,0.1,0,300,25,25 @08:00:07.05 PCR.caltemp= 6.6661,0.0344,0.0 @08:00:07.15 Intake.select :close @08:00:07.18 Exhaust.select :close CTV.dial :air @08:00:07.22 PSV.dial :air @08:00:07.25
PC.reconfigure PCconfig @08:00:07.30 PC.home.seek open @08:00:07.39 CSV.dial :waste1 @08:00:07.81 SSV.dial :spit,avoiding => exhaust @08:00:07.88 PS.reconfigure PSconfig @08:00:07.93 PS.home.seek 0.000ml @08:00:08.67 CS.reconfigure CSfast @08:00:08.70 SS.reconfigure SSconfig @08:00:08.79 SS.home.seek 0.00ml @08:00:08.81 CS.home.seek 0.000ml @08:00:08.88 CC.reconfigure CCconfig @08:00:08.92 CC.home.seek open @08:00:18.21 PS.coast @08:00:18.30
CC.seek open @08:00:18.32 PC.seek open @08:00:18.48
Forearm.reconfigure LooseForearm @08:00:18.51 Forearm.home.seek retract @08:00:19.67 Hand.grip @08:00:19.70
Elbow.jog -1500 @08:00:20.21 Elbow.reconfigure LooseElbow @08:00:20.25 Elbow.home.seek clear @08:00:24.99 SE.reconfigure SEconfig @08:00:25.02 SE.home.seek down @08:00:25.56 SC.reconfigure SCsloppy @08:00:25.60 SC.home.seek initial @08:00:27.75 Forearm.seek retract @08:00:27.80 Elbow.seek hammer @08:00:28.95 Forearm.reconfigure TightForearm @08:00:29.00 Forearm.seek 2 @08:00:30.05 Forearm.seek retract @08:00:31.14 Forearm.reconfigure LooseForearm @08:00:31.51PST06-Mar-14 ===> STARTED wqPCR (phase #3) <=== @08:00:31.52 Starting initialPurge @08:00:31.53 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:00:31.58 Hand.release @08:00:32.43 Elbow.reconfigure TightElbow @08:00:32.47 Elbow.seek FlushPuck::Garage @08:00:34.24 Elbow.reconfigure LooseElbow @08:00:34.33 Forearm.seek garage @08:00:35.37 Hand.grip @08:00:36.19 Forearm.seek retract @08:00:37.24 Elbow.reconfigure TightElbow @08:00:37.28 Elbow.seek CC @08:00:40.04 Elbow.reconfigure LooseElbow @08:00:40.12 Forearm.reconfigure TightForearm @08:00:40.15 Forearm.seek CC @08:00:41.25 CC.seek closed @08:00:44.98 Collection Clamp onSpring/offSpring for 5/22 samples, on/off torque 1.92 @08:00:45.03 Hand.release @08:00:45.76 Forearm.reconfigure LooseForearm @08:00:45.79 Forearm.seek retract @08:00:46.78 Flushing the Collection Valve::Manifold @08:00:46.87 CS Pulling 5ml of flush @08:00:46.88 CSV.dial CSR @08:00:48.14 CSR.select :flush @08:00:48.24 CS.reconfigure CSconfig @08:00:48.27 CS.seek 5.000ml @08:01:01.80 delay 5 seconds @08:01:06.82 CSR.select :air @08:01:06.96 CS.reconfigure CSfast @08:01:06.99 CS.seek 7.500ml @08:01:13.67 CSV.dial :waste @08:01:14.93 CS.reconfigure CSconfig @08:01:14.96 CS.seek 0.000ml @08:01:34.83 Purging external Intake @08:01:34.92 CSV.dial :flush @08:01:35.37 CS.reconfigure CSfast @08:01:35.40 CS.seek 10.000ml @08:01:59.29 delay 5 seconds @08:02:04.31 CSV.dial CBV @08:02:04.33
CBV.dial CSV @08:02:05.15 CTV.dial :intake @08:02:05.86 Intake.select :open @08:02:05.95 CS.seek 0.000ml @08:02:30.20 Intake.select :close @08:02:30.29 CTV.dial :air @08:02:31.06 Flushing upper manifold @08:02:31.16 CSV.dial :flush @08:02:31.94 CS.seek 5.000ml @08:02:44.32 delay 5 seconds @08:02:49.33 CSV.dial :air @08:02:49.81 CS.seek 7.000ml @08:02:55.29 CSV.dial CBV @08:02:55.31
CBV.dial CSV @08:02:56.38 CTV.dial :air @08:02:56.40 CS.seek 0.000ml @08:03:13.23 delay 7 seconds @08:03:20.24 CS.seek 3.000ml @08:03:27.98 CSV.dial :waste @08:03:28.49 CS.seek 0.000ml @08:03:36.27 CSV.dial :flush @08:03:36.70 CS.seek 3.000ml @08:03:44.44 delay 5 seconds @08:03:49.46 CSV.dial :air @08:03:49.93 CS.seek 5.000ml @08:03:55.44 CSV.dial :air @08:03:55.45 CS.seek 0.000ml @08:04:07.66 delay 7 seconds @08:04:14.68 CS.seek 3.000ml @08:04:22.45 CSV.dial :waste @08:04:23.24 CS.seek 0.000ml @08:04:30.98 Garaging FlushPuck in Collection Clamp @08:04:31.03 Elbow.reconfigure TightElbow @08:04:31.07 Elbow.seek CC @08:04:31.28 Elbow.reconfigure LooseElbow @08:04:31.37 Forearm.seek CC @08:04:32.35 CC.seek sealed @08:04:33.30 Hand.grip @08:04:34.10 CC.seek open @08:04:37.39 Forearm.seek retract @08:04:38.32 Elbow.reconfigure TightElbow @08:04:38.36 Elbow.seek garage @08:04:41.14 Elbow.reconfigure LooseElbow @08:04:41.23 Forearm.reconfigure TightForearm @08:04:41.81 Forearm.seek garage @08:04:42.98 Hand.release @08:04:43.76 Forearm.reconfigure LooseForearm @08:04:43.79 Forearm.seek retract @08:04:44.87 Starting mfb @08:04:44.88 pcrBAC sampling at most 1000ml, wcr at most 1000ml @08:04:44.90 BACfans.email "Can@08:00:31, 15.6C, 61% humidity, 14.2psia, 13.729V, 0.598A, 0.604A avg, 8.20W\nCTD data unavailable\nISUS data unavailable",Subject => "pcrBAC sampling at most 1000ml, wcr at most 1000ml" @08:04:44.92
Loading sh1 puck from tube 2 into Collection Clamp @08:04:46.64 Readying the MFB @08:04:46.67 Homing MFB Syringe AFV.select :holdingCoil @08:04:46.89 ATV.dial :air2 @08:04:46.93
SC.reconfigure SCloose @08:04:47.10 Elbow.reconfigure TightElbow @08:04:47.13 Elbow.seek 2 @08:04:48.18 AS.reconfigure ASconfig @08:04:48.21 AS.home.seek home @08:04:48.95
Forearm.reconfigure TightForearm @08:04:48.98 Forearm.seek 2 + 800 counts @08:04:50.04 SE.reconfigure SEpick @08:04:50.09 SE.seek beamBreak @08:04:51.54 SE.configure SEconfig @08:04:51.67 Elbow.seek 2 @08:04:51.83 Forearm.seek 2 + 300 counts @08:04:52.17 Forearm.reconfigure LooseForearm @08:04:52.21 Hand.grip @08:04:52.99 SE.seek down @08:04:53.11
Forearm.seek retract @08:04:54.18 Elbow.seek CC @08:04:54.31 SE.coast @08:04:55.70
Elbow.reconfigure LooseElbow @08:04:55.75 AFV.select :carrierSolution @08:04:55.81
Forearm.reconfigure TightForearm @08:04:55.86 Forearm.seek CC @08:04:55.90 delay 2 seconds @08:04:56.91
CC.seek closed @08:04:57.91 AS.seek 1.000ml @08:05:00.67
Collection Clamp onSpring/offSpring for 4/21 samples, on/off torque 1.99 @08:05:00.68 Hand.release @08:05:01.51 Forearm.reconfigure LooseForearm @08:05:01.54 Forearm.seek retract @08:05:02.48 SC.coast @08:05:02.51 collectSample 1000ml w/bubble point of 55psi Priming 7ml @08:05:02.52 Sleepy.power :sampler,:ON @08:05:02.53 Intake.select :close Exhaust.select :close @08:05:02.54 SS.hold @08:05:02.70 Calibrating pressure and temperature sensors @08:05:02.72 CTV.dial :air @08:05:02.73 SSV.dial :spit,avoiding => exhaust @08:05:02.74 SS.reconfigure SSfast @08:05:02.77 SS.seek 5.00ml @08:05:05.89 SSV.dial CBV @08:05:05.91
CBV.dial SSV @08:05:07.29 delay 3.8 seconds @08:05:11.17 SSV.dial :spit @08:05:12.50 SS.seek 0.00ml @08:05:15.20 SSV.dial CBV @08:05:16.54 CTV.dial :intake @08:05:17.31 Waiting for external sampler to finish priming... @08:05:17.32 delayUntil "08:15:02.52" @08:05:49.62 delay 1 second @08:05:50.63 AFV.select :holdingCoil @08:05:50.72 delay 1 second @08:05:51.73 ATV.dial :waste @08:05:52.64 AS.reconfigure ASnorm @08:05:52.68 AS.home.seek empty @08:06:45.79 ATV.dial :waste @08:06:45.80 ARV.dial between carrier and air1 @08:06:45.87 ASV.dial :waste1 @08:15:02.52 SSV.dial CBV @08:15:02.54
CBV.dial SSV @08:15:02.55 Allowing 55s for 7.0ml of remaining 7.0ml Intake.select :open @08:15:04.68 Intake @25.1psia, vacuum from 18.6 to 22.6psi @08:15:04.69 SS.reconfigure forSampling @08:15:04.73 SS.seek 7.00ml @08:15:09.67 Stroke completed -- waiting for pressure to equalize ... @08:15:10.31 Intake.select :close @08:15:10.44 SSV.dial :exhaust @08:15:11.21 Exhaust.select :open @08:15:11.31 SS.reconfigure SSexhale @08:15:11.35 SS.seek 0.00ml @08:15:14.76 Exhaust.select :close @08:15:14.86 Sampling 1000.0ml @08:15:14.87 Intake.select :close @08:15:14.88 Exhaust.select :close @08:15:14.89 SS.reconfigure SSconfig @08:15:14.92 SS.hold @08:15:15.07 CTV.dial :intake @08:15:15.08 SSV.dial CBV @08:15:15.10
CBV.dial SSV @08:15:15.88 Allowing 185s for 25.0ml of remaining 1000.0ml Intake.select :open @08:15:18.01 Intake @24.8psia, vacuum from 18.3 to 22.3psi @08:15:18.02 SS.reconfigure forSampling @08:15:18.06 SS.seek 25.00ml @08:15:31.92 Stroke completed -- waiting for pressure to equalize ... @08:15:32.55 Intake.select :close @08:15:32.69 SSV.dial :exhaust @08:15:33.46 Exhaust.select :open @08:15:33.55 SS.reconfigure SSexhale @08:15:33.59 SS.seek 0.00ml @08:15:42.18 Exhaust.select :close @08:15:42.27 SSV.dial CBV @08:15:42.29
CBV.dial SSV @08:15:43.07 Allowing 185s for 25.0ml of remaining 975.0ml @08:15:43.08 Intake.select :open @08:15:45.21 Intake @26.3psia, vacuum from 19.8 to 23.8psi @08:15:45.22 SS.reconfigure forSampling @08:15:45.26 SS.seek 25.00ml @08:15:52.43 Vacuum too high -- waiting up to 40s @08:15:52.70 Intake @17.3psia, vacuum from 10.8 to 14.8psi @08:15:52.71 SS.reconfigure forSampling @08:15:52.75 SS.seek 25.00ml @08:15:57.30 Vacuum too high -- waiting up to 40s @08:15:58.20 Intake @20.7psia, vacuum from 14.2 to 18.2psi @08:15:58.21 SS.reconfigure forSampling @08:15:58.25 SS.seek 25.00ml @08:16:02.38 Stroke completed -- waiting for pressure to equalize ... @08:16:03.02 Intake.select :close @08:16:03.15 SSV.dial :exhaust @08:16:03.91 Exhaust.select :open @08:16:04.00 SS.reconfigure SSexhale @08:16:04.04 SS.seek 0.00ml @08:16:12.60 Exhaust.select :close @08:16:12.69 SSV.dial CBV @08:16:12.71
CBV.dial SSV @08:16:13.49 Allowing 185s for 25.0ml of remaining 950.0ml @08:16:13.50 Intake.select :open @08:16:15.63 Intake @25.0psia, vacuum from 18.5 to 22.5psi @08:16:15.64 SS.reconfigure forSampling @08:16:15.68 SS.seek 25.00ml @08:16:19.86 Vacuum too high -- waiting up to 40s @08:16:20.13 Intake @20.4psia, vacuum from 13.9 to 17.9psi @08:16:20.14 SS.reconfigure forSampling @08:16:20.17 SS.seek 25.00ml @08:16:22.75 Vacuum too high -- waiting up to 40s @08:16:23.43 Intake @21.0psia, vacuum from 14.5 to 18.5psi @08:16:23.44 SS.reconfigure forSampling @08:16:23.48 SS.seek 25.00ml @08:16:27.43 Vacuum too high -- waiting up to 40s @08:16:28.31 Intake @21.2psia, vacuum from 14.7 to 18.7psi @08:16:28.32 SS.reconfigure forSampling @08:16:28.36 SS.seek 25.00ml @08:16:33.08 Vacuum too high -- waiting up to 40s @08:16:33.76 Intake @21.1psia, vacuum from 14.6 to 18.6psi @08:16:33.77 SS.reconfigure forSampling @08:16:33.81 SS.seek 25.00ml @08:16:41.02 Vacuum too high -- waiting up to 40s @08:16:41.91 Intake @21.9psia, vacuum from 15.4 to 19.4psi @08:16:41.92 SS.reconfigure forSampling @08:16:41.96 SS.seek 25.00ml @08:16:46.12 Stroke completed -- waiting for pressure to equalize ... @08:16:46.75 Intake.select :close @08:16:46.89 SSV.dial :exhaust @08:16:47.66 Exhaust.select :open @08:16:47.75 SS.reconfigure SSexhale @08:16:47.79 SS.seek 0.00ml @08:16:56.24 Exhaust.select :close @08:16:56.33 SSV.dial CBV @08:16:56.35
CBV.dial SSV @08:16:57.13 Allowing 185s for 25.0ml of remaining 925.0ml @08:16:57.14 Intake.select :open @08:16:59.27 Intake @23.5psia, vacuum from 17.0 to 21.0psi @08:16:59.28 SS.reconfigure forSampling @08:16:59.32 SS.seek 25.00ml @08:17:08.70 Vacuum too high -- waiting up to 40s @08:17:09.72 Intake @21.9psia, vacuum from 15.4 to 19.4psi @08:17:09.73 SS.reconfigure forSampling @08:17:09.76 SS.seek 25.00ml @08:17:41.58 Vacuum too high -- waiting up to 40s @08:17:43.14 Intake @21.8psia, vacuum from 15.3 to 19.3psi @08:17:43.15 SS.reconfigure forSampling @08:17:43.18 SS.seek 25.00ml @08:18:10.06 Stroke completed -- waiting for pressure to equalize ... @08:18:11.86 Intake.select :close @08:18:12.00 SSV.dial :exhaust @08:18:12.77 Exhaust.select :open @08:18:12.87 SS.reconfigure SSexhale @08:18:12.91 SS.seek 0.00ml @08:18:21.78 Exhaust.select :close @08:18:21.87 SSV.dial CBV @08:18:21.89
CBV.dial SSV @08:18:22.67 Allowing 185s for 25.0ml of remaining 900.0ml @08:18:22.68 Intake.select :open @08:18:24.81 Intake @22.4psia, vacuum from 15.9 to 19.9psi @08:18:24.82 SS.reconfigure forSampling @08:18:24.86 SS.seek 25.00ml @08:19:39.51 Vacuum too high -- waiting up to 40s @08:19:41.90 Intake @21.4psia, vacuum from 14.9 to 18.9psi @08:19:41.91 SS.seek 25.00ml @08:19:53.98 Vacuum too high -- waiting up to 40s @08:19:56.78 Intake @20.8psia, vacuum from 14.3 to 18.3psi @08:19:56.79 SS.seek 25.00ml @08:20:07.51 Vacuum too high -- waiting up to 40s @08:20:11.14 Intake @21.2psia, vacuum from 14.7 to 18.7psi @08:20:11.16 SS.seek 25.00ml @08:20:21.01 Vacuum too high -- waiting up to 40s @08:20:24.64 Intake @21.0psia, vacuum from 14.5 to 18.5psi @08:20:24.66 SS.seek 25.00ml @08:20:28.48 Stroke completed -- waiting for pressure to equalize ... @08:20:33.86 Intake.select :close @08:20:33.99 SSV.dial :exhaust @08:20:34.74 Exhaust.select :open @08:20:34.83 SS.reconfigure SSexhale @08:20:34.87 SS.seek 0.00ml @08:20:43.76 Exhaust.select :close @08:20:43.85 SSV.dial CBV @08:20:43.87
CBV.dial SSV @08:20:44.63 Allowing 185s for 25.0ml of remaining 875.0ml @08:20:44.64 Intake.select :open @08:20:46.77 Intake @21.3psia, vacuum from 14.8 to 18.8psi @08:20:46.78 SS.reconfigure forSampling @08:20:46.82 SS.seek 25.00ml @08:20:55.47 Vacuum too high -- waiting up to 40s @08:20:57.85 Intake @20.6psia, vacuum from 14.1 to 18.1psi @08:20:57.87 SS.seek 25.00ml @08:21:03.16 Vacuum too high -- waiting up to 40s @08:21:05.77 Intake @21.2psia, vacuum from 14.7 to 18.7psi @08:21:05.79 SS.seek 25.00ml @08:21:11.63 Vacuum too high -- waiting up to 40s @08:21:14.22 Intake @20.8psia, vacuum from 14.3 to 18.3psi @08:21:14.24 SS.seek 25.00ml @08:21:19.48 Vacuum too high -- waiting up to 40s @08:21:22.48 Intake @20.9psia, vacuum from 14.4 to 18.4psi @08:21:22.50 SS.seek 25.00ml @08:21:28.33 Vacuum too high -- waiting up to 40s @08:21:31.52 Intake @21.5psia, vacuum from 15.0 to 19.0psi @08:21:31.54 SS.seek 25.00ml @08:21:37.04 Vacuum too high -- waiting up to 40s @08:21:40.09 Intake @20.9psia, vacuum from 14.4 to 18.4psi @08:21:40.10 SS.seek 25.00ml @08:21:45.64 Vacuum too high -- waiting up to 40s @08:21:49.39 Intake @22.3psia, vacuum from 15.8 to 19.8psi @08:21:49.40 SS.seek 25.00ml @08:21:56.54 Vacuum too high -- waiting up to 40s @08:22:00.42 Intake @22.6psia, vacuum from 16.1 to 20.1psi @08:22:00.43 SS.seek 25.00ml @08:22:06.76 Vacuum too high -- waiting up to 40s @08:22:10.18 Intake @21.8psia, vacuum from 15.3 to 19.3psi @08:22:10.20 SS.seek 25.00ml @08:22:15.73 Vacuum too high -- waiting up to 40s @08:22:19.77 Intake @21.3psia, vacuum from 14.8 to 18.8psi @08:22:19.79 SS.seek 25.00ml @08:22:25.42 Vacuum too high -- waiting up to 40s @08:22:29.97 Intake @21.4psia, vacuum from 14.9 to 18.9psi @08:22:29.99 SS.seek 25.00ml @08:22:35.77 Vacuum too high -- waiting up to 40s @08:22:40.31 Intake @21.1psia, vacuum from 14.6 to 18.6psi @08:22:40.32 SS.seek 25.00ml @08:22:45.92 Vacuum too high -- waiting up to 40s @08:22:51.05 Intake @21.3psia, vacuum from 14.8 to 18.8psi @08:22:51.07 SS.seek 25.00ml @08:22:56.92 Vacuum too high -- waiting up to 40s @08:23:02.02 Intake @22.0psia, vacuum from 15.5 to 19.5psi @08:23:02.04 SS.seek 25.00ml @08:23:08.13 Vacuum too high -- waiting up to 40s @08:23:13.75 Intake @22.6psia, vacuum from 16.1 to 20.1psi @08:23:13.76 SS.seek 25.00ml @08:23:20.54 Vacuum too high -- waiting up to 40s @08:23:25.92 Intake @21.9psia, vacuum from 15.4 to 19.4psi @08:23:25.93 SS.seek 25.00ml @08:23:32.19 Vacuum too high -- waiting up to 40s @08:23:38.37 Intake @22.4psia, vacuum from 15.9 to 19.9psi @08:23:38.38 SS.seek 25.00ml @08:23:45.30 Vacuum too high -- waiting up to 40s @08:23:49.71 SS.reconfigure SSconfig @08:23:49.74 SS.hold @08:23:49.83 Intake.select :close @08:23:49.98 ShallowSampler::Clogged in MAIN -- progress too slow after 149.3ml @08:23:49.99 SSV.dial :exhaust @08:23:50.74 Exhaust.select :open @08:23:50.84 SS.reconfigure SSexhale @08:23:50.88 SS.seek 0.00ml @08:23:59.63 Exhaust.select :close @08:23:59.73 Sampled 149.3ml @08:23:59.74 collectSample: Complete Evacuating Puck (bubblePt=55.0psi, delay=15.0s) @08:23:59.75 Intake.select :close @08:23:59.81 CSV.dial :waste @08:23:59.84 CS.seek 0.000ml @08:23:59.92 SSV.dial CBV,avoiding => exhaust @08:23:59.95
CBV.dial SSV @08:23:59.99 CSV.dial :air @08:24:00.80 CS.seek 10.000ml @08:24:01.83
CTV.dial :air @08:24:02.72 Sleepy.power :sampler,:OFF @08:24:03.26
delay 15 seconds @08:24:18.26 SSV.dial :spit,avoiding => exhaust @08:24:19.58 CTV.dial CSV,avoiding => intake @08:24:19.60
SS.seek 0.00ml @08:24:19.70 SSV.dial CBV,avoiding => exhaust @08:24:25.30 CBV.dial between CSV and waste2b @08:24:26.24 CTV.dial CSV,avoiding => air @08:24:26.26
CSV.dial CTV @08:24:26.70 CS.reconfigure CSpressurize @08:24:26.73 CS.seek 0.000ml @08:24:52.60 CSV.dial :air @08:24:53.11 CS.reconfigure CSfast @08:24:53.14 CS.seek 10.000ml @08:25:16.76 CSV.dial between air and CTV @08:25:17.14 CS.seek 2.420ml @08:25:35.29 CTV.dial CSV,avoiding => air @08:25:35.30
CSV.dial CTV @08:25:35.81 CS.reconfigure CSpressurize @08:25:35.84 CS.seek 0.000ml @08:25:38.55 BubblePoint: Intake @69.7psia, Outlet @14.9psia @08:25:38.56 SS.reconfigure SSconfig @08:25:38.59 SS.seek 10.00ml @08:25:44.41 delay 15 seconds @08:25:59.51 Evacuated: Intake @68.7psia, Outlet @-0.1psia SSV.dial :spit,avoiding => exhaust @08:26:00.84 SS.reconfigure SSexhale @08:26:00.87 SS.seek 0.00ml @08:26:05.83 SS.reconfigure SSfast @08:26:05.87 SS.seek 5.00ml @08:26:08.79 SSV.dial CBV,avoiding => exhaust @08:26:08.81
CBV.dial SSV @08:26:10.14 CTV.dial :air,avoiding => intake @08:26:11.44 CSV.dial CTV @08:26:11.46
SS.reconfigure SSconfig @08:26:11.50 SS.seek 10.00ml @08:26:12.46 CSV.dial :air @08:26:12.90 CS.reconfigure CSfast @08:26:12.93 CS.seek 0.000ml @08:26:14.74
SSV.dial :spit,avoiding => exhaust @08:26:16.06 SS.reconfigure SSexhale @08:26:16.09 SS.seek 0.00ml @08:26:17.47 CS.coast @08:26:21.19
SS.reconfigure SSfast @08:26:21.23 SS.seek 5.00ml @08:26:24.40 SSV.dial CBV,avoiding => exhaust @08:26:24.42
CBV.dial SSV @08:26:26.72 SSV.dial :spit,avoiding => exhaust @08:26:28.03 SS.reconfigure SSconfig @08:26:28.06 SS.seek 0.00ml @08:26:31.13 SS.coast @08:26:31.16 Evacuating Puck (bubblePt=55.0psi, delay=15.0s) Intake.select :close @08:26:31.22 CSV.dial :waste @08:26:31.31
SSV.dial :exhaust @08:26:31.96 CS.seek 0.000ml @08:26:32.06
Exhaust.select :open @08:26:32.17 SS.reconfigure SSexhale @08:26:32.21 SS.seek 0.00ml @08:26:32.28 CSV.dial :air @08:26:32.63
Exhaust.select :close @08:26:32.73 SSV.dial CBV,avoiding => exhaust @08:26:32.75
CBV.dial SSV @08:26:33.27 CS.seek 10.000ml @08:26:34.64
CTV.dial :air @08:26:34.65 delay 15 seconds @08:26:49.65 SSV.dial :spit,avoiding => exhaust @08:26:50.98 CTV.dial CSV,avoiding => intake @08:26:51.00
SS.seek 0.00ml @08:26:51.09 SSV.dial CBV,avoiding => exhaust @08:26:57.27 CBV.dial between CSV and waste2b @08:26:58.23 CSV.dial between air and CTV @08:26:58.60 CS.seek 7.601ml @08:27:04.91 CTV.dial CSV,avoiding => air @08:27:04.92
CSV.dial CTV @08:27:05.43 CS.reconfigure CSpressurize @08:27:05.46 CS.seek 0.000ml @08:27:25.28 CSV.dial :air @08:27:25.71 CS.reconfigure CSfast @08:27:25.74 CS.seek 10.000ml @08:27:49.79 CSV.dial between air and CTV @08:27:50.18 CS.seek 2.893ml @08:28:07.19 CTV.dial CSV,avoiding => air @08:28:07.20
CSV.dial CTV @08:28:07.49 CS.reconfigure CSpressurize @08:28:07.52 CS.seek 0.000ml @08:28:13.76 BubblePoint: Intake @70.0psia, Outlet @14.6psia SS.reconfigure SSconfig @08:28:13.80 SS.seek 10.00ml @08:28:19.67 delay 15 seconds @08:28:34.77 Evacuated: Intake @68.6psia, Outlet @0.2psia SSV.dial :spit,avoiding => exhaust @08:28:36.63 SS.reconfigure SSexhale @08:28:36.67 SS.seek 0.00ml @08:28:41.60 SS.reconfigure SSfast @08:28:41.64 SS.seek 5.00ml @08:28:44.55 SSV.dial CBV,avoiding => exhaust @08:28:44.57
CBV.dial SSV @08:28:45.91 CTV.dial :air,avoiding => intake @08:28:47.21 CSV.dial CTV @08:28:47.23
SS.reconfigure SSconfig @08:28:47.27 SS.seek 10.00ml @08:28:48.23 CSV.dial :air @08:28:48.75 CS.reconfigure CSfast @08:28:48.78 CS.seek 0.000ml @08:28:50.48
SSV.dial :spit,avoiding => exhaust @08:28:51.05 CS.coast @08:28:51.80
SS.reconfigure SSexhale @08:28:51.83 SS.seek 0.00ml @08:28:56.90 SS.reconfigure SSfast @08:28:56.93 SS.seek 5.00ml @08:28:59.82 SSV.dial CBV,avoiding => exhaust @08:28:59.84
CBV.dial SSV @08:29:02.15 SSV.dial :spit,avoiding => exhaust @08:29:03.47 SS.reconfigure SSconfig @08:29:03.50 SS.seek 0.00ml @08:29:06.73 SS.coast @08:29:06.76 Lysing: Adding 1.5ml lysis CBV.dial between CSV and waste1,avoiding => DBV @08:29:08.25 CTV.dial CSV @08:29:09.51 CSV.dial CSR @08:29:10.30 CSR.select :lysis @08:29:10.40 CS.reconfigure CSconfig @08:29:10.44 CS.seek 1.000ml @08:29:13.80 delay 5 seconds @08:29:18.82 CSR.select :air @08:29:18.96 CS.seek 6.000ml @08:29:32.40 delay 2 seconds @08:29:34.42 CSV.dial :waste1 @08:29:35.69 CS.reconfigure CSfast @08:29:35.72 CS.seek empty @08:29:50.15 CSV.dial CSR @08:29:51.43 CSR.select :lysis @08:29:51.53 CS.reconfigure CSconfig @08:29:51.57 CS.seek 1.500ml @08:29:56.30 delay 5 seconds @08:30:01.31 CSR.select :air @08:30:01.45 CS.seek 4.501ml @08:30:09.83 delay 2 seconds @08:30:11.84 CSV.dial CTV @08:30:12.36 CS.seek 1.275ml @08:30:21.31 Heating sh1 puck from tube 2 in Collection Clamp to 85.0C for 5:00 @08:30:21.32 CH.seek 85.0C,8:00 @08:33:27.21 CH.hold 5:00,85.0C @08:38:27.33 Collection Puck Heater at 85.0C after lysing @08:38:27.34 CS.seek 0.000ml @08:38:31.45 CTV.dial between spare and air,avoiding => intake @08:38:32.43 CSV.dial :air @08:38:32.86 CS.seek 5.000ml @08:38:46.32 CSV.dial CTV @08:38:46.82 delay 2 seconds @08:38:48.83 CSV.dial :air @08:38:49.33 delay 2 seconds @08:38:51.34 CSV.dial CTV @08:38:51.83 delay 2 seconds @08:38:53.84 CSV.dial :air @08:38:54.33 delay 2 seconds @08:38:56.34 CS.seek 0.000ml @08:39:09.82 Recovering Lysate into Collection syringe @08:39:09.83 CSV.dial CBV @08:39:10.82 CBV.dial CSV @08:39:11.16 CS.seek 5.000ml @08:39:24.75 delay 5 seconds @08:39:29.76 CTV.dial :air @08:39:30.24 CS.seek 7.001ml @08:39:36.07 delay 5 seconds @08:39:41.08 CSV.dial :air2 @08:39:41.51 CS.seek 10.000ml @08:39:49.91 CSV.dial between air and CTV,avoiding => CBV @08:39:51.03 Lysing Complete @08:39:51.08 Storing sh1 puck in Collection Clamp to tube 1 @08:39:51.20 SC.reconfigure SCtight @08:39:51.23 SC.seek 1 @08:39:53.48 SC.reconfigure SCloose @08:39:53.65 Elbow.reconfigure TightElbow @08:39:53.68 Elbow.seek 1 @08:39:55.31 Forearm.reconfigure TightForearm @08:39:55.34 Forearm.seek 1 + 800 counts @08:39:56.43 SE.reconfigure SEpick @08:39:56.48 SE.seek beamBreak @08:39:59.00 SE.configure SEconfig @08:39:59.12 Elbow.reconfigure LimpElbow @08:39:59.15 Forearm.reconfigure LooseForearm @08:39:59.19 Forearm.seek 1 @08:39:59.42 Elbow.hold @08:39:59.47 Elbow.reconfigure TightElbow @08:39:59.55 SE.seek 5.05 Pucks below Top Plate @08:39:59.86 Forearm.coast @08:39:59.89 Forearm.seek 1 + 330 counts @08:40:00.13 Forearm.reconfigure TightForearm @08:40:00.16 Forearm.seek 1 + 800 counts @08:40:00.40 SE.seek 6.05 Pucks below Top Plate @08:40:00.96 Forearm.reconfigure LooseForearm @08:40:01.64 Forearm.seek retract @08:40:02.59 Elbow.seek CC @08:40:04.15 Elbow.reconfigure LooseElbow @08:40:04.24 Forearm.seek CC @08:40:05.18 CC.seek sealed @08:40:06.13 Hand.grip @08:40:06.90 CC.seek open @08:40:10.23 Forearm.seek retract @08:40:11.19 Elbow.reconfigure TightElbow @08:40:11.23 Elbow.seek 1 @08:40:12.83 Elbow.reconfigure LimpElbow @08:40:12.87 Forearm.seek 1 @08:40:13.86 Elbow.hold @08:40:13.91 Elbow.reconfigure TightElbow @08:40:13.95 Hand.release @08:40:14.73 Forearm.coast @08:40:14.76 Forearm.seek 1 + 330 counts @08:40:15.01 Forearm.reconfigure TightForearm @08:40:15.04 Forearm.seek 1 + 800 counts @08:40:15.25 SE.seek down @08:40:16.36
Forearm.reconfigure LooseForearm @08:40:16.39 Forearm.seek retract @08:40:17.43 SE.coast @08:40:17.46
SC.coast @08:40:17.48 Tranferring lysate to PRV @08:40:17.49 CSV.dial :air @08:40:17.78 CS.seek 10.000ml @08:40:17.85 CSV.dial between air and CTV @08:40:18.23 AIV.dial :bypass @08:40:18.28 Beginning samplePassC @08:40:18.29 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:40:18.44 Elbow.seek FlushPuck::Garage @08:40:20.13 Elbow.reconfigure LooseElbow @08:40:20.21 Forearm.seek garage @08:40:21.23 Hand.grip @08:40:22.11 Forearm.seek retract @08:40:23.12 Elbow.reconfigure TightElbow @08:40:23.16 Elbow.seek CC @08:40:25.90 Elbow.reconfigure LooseElbow @08:40:25.99 Forearm.reconfigure TightForearm @08:40:26.02 Forearm.seek CC @08:40:27.11 CC.seek closed @08:40:30.91 Collection Clamp onSpring/offSpring for 5/22 samples, on/off torque 2.01 @08:40:30.96 Hand.release @08:40:31.69 Forearm.reconfigure LooseForearm @08:40:31.72 Forearm.seek retract @08:40:32.69 AWV.select :air @08:40:32.70 AIV.dial :bypass @08:40:32.71 PSV.dial PSR @08:40:33.98 PSR.select :flush @08:40:34.08 PS.seek 1.500ml @08:40:50.17 delay 5 seconds @08:40:55.19 PSV.dial :waste1 @08:40:55.63 PS.seek 0.000ml @08:41:11.79 PSV.dial PSR @08:41:12.25 PSR.select :air @08:41:12.35 PS.seek 2.500ml @08:41:38.58 CBV.dial PRV @08:41:38.59 PRV.dial CBV @08:41:39.34
PSV.dial :mixloop @08:41:40.34 CTV.dial :air,avoiding => intake @08:41:40.35 PTV.dial :PRVmixing @08:41:40.43 PS.seek 0.000ml @08:42:06.64 PSV.dial :air @08:42:07.08 PS.seek 2.500ml @08:42:33.35 PSV.dial :mixloop @08:42:33.82 PS.seek 0.000ml @08:43:00.07 PSV.dial :air @08:43:00.53 PS.seek 2.500ml @08:43:26.76 PSV.dial :mixloop @08:43:27.22 PS.seek 0.000ml @08:43:54.07 Garaging FlushPuck in Collection Clamp @08:43:54.13 Elbow.reconfigure TightElbow @08:43:54.16 Elbow.seek CC @08:43:54.25 Elbow.reconfigure LooseElbow @08:43:54.34 Forearm.seek CC @08:43:55.28 CC.seek sealed @08:43:56.21 Hand.grip @08:43:56.89 CC.seek open @08:44:00.18 Forearm.seek retract @08:44:01.12 Elbow.reconfigure TightElbow @08:44:01.16 Elbow.seek garage @08:44:03.98 Elbow.reconfigure LooseElbow @08:44:04.06 Forearm.reconfigure TightForearm @08:44:04.09 Forearm.seek garage @08:44:05.27 Hand.release @08:44:06.06 Forearm.reconfigure LooseForearm @08:44:06.09 Forearm.seek retract @08:44:07.11 Connecting PS to CS to transfer sample @08:44:07.12 CBV.dial AIV @08:44:07.13 AIV.dial :bypass @08:44:07.14 PSV.dial :mixloop @08:44:07.15 PTV.dial :PRVmixing PRV.dial AIV @08:44:07.16 CTV.dial :air,avoiding => intake @08:44:07.17 Loading lyfil puck from tube 2 into Collection Clamp @08:44:07.34 SC.reconfigure SCtight @08:44:07.37 SC.seek 2 @08:44:09.56 SC.reconfigure SCloose @08:44:09.73 Elbow.reconfigure TightElbow @08:44:09.76 Elbow.seek 2 @08:44:11.67 Forearm.reconfigure TightForearm @08:44:11.70 Forearm.seek 2 + 800 counts @08:44:12.77 SE.reconfigure SEpick @08:44:12.82 SE.seek beamBreak @08:44:14.38 SE.configure SEconfig @08:44:14.50 Elbow.seek 2 @08:44:14.70 Forearm.seek 2 + 300 counts @08:44:14.99 Forearm.reconfigure LooseForearm @08:44:15.03 Hand.grip @08:44:15.78 SE.seek down @08:44:15.92
Forearm.seek retract @08:44:16.94 Elbow.seek CC @08:44:17.14 SE.coast @08:44:18.48
Elbow.reconfigure LooseElbow @08:44:18.56 Forearm.reconfigure TightForearm @08:44:18.59 Forearm.seek CC @08:44:19.71 CC.seek closed @08:44:23.52 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 2.01 @08:44:23.53 Hand.release @08:44:24.30 Forearm.reconfigure LooseForearm @08:44:24.33 Forearm.seek retract @08:44:25.32 SC.coast @08:44:25.35 CTV.dial CSV,avoiding => intake @08:44:26.65 CSV.dial CTV @08:44:27.16 CS.seek 5.000ml @08:44:40.65 delay 1:20 @08:46:00.66 CS.seek 2.500ml @08:46:07.86 delay 40 seconds @08:46:47.87 CS.seek 0.000ml @08:46:55.08 delay 1:00 @08:47:55.09 PSV.dial :air @08:47:55.55 PS.seek 2.500ml @08:48:21.81 PSV.dial :mixloop @08:48:22.26 delay 3 seconds @08:48:25.27 PSV.dial :air @08:48:25.70 delay 2 seconds @08:48:27.71 PSV.dial :mixloop @08:48:28.15 delay 3 seconds @08:48:31.16 PSV.dial :waste1 @08:48:32.44 PS.seek empty @08:48:58.75 CBV.dial :waste1 @08:48:59.21 delay 5 seconds @08:49:04.22 CBV.dial AIV @08:49:04.67 delay 10 seconds @08:49:14.68 CBV.dial :waste1 @08:49:15.14 delay 10 seconds @08:49:25.15 CBV.dial AIV @08:49:25.60 delay 10 seconds @08:49:35.61 CTV.dial CSV,avoiding => intake @08:49:35.62 CBV.dial between AIV and waste1 @08:49:35.95 CSV.dial :air @08:49:36.38 CS.seek 10.000ml @08:50:02.48 CSV.dial CTV @08:50:02.99 CS.seek 0.000ml @08:50:29.63 CBV.dial :waste1 @08:50:29.94 delay 10 seconds @08:50:39.95 CTV.dial :air,avoiding => intake @08:50:41.28 Storing lyfil puck in Collection Clamp to tube 1 @08:50:41.41 SC.reconfigure SCtight @08:50:41.44 SC.seek 1 @08:50:43.54 SC.reconfigure SCloose @08:50:43.71 Elbow.reconfigure TightElbow @08:50:43.75 Elbow.seek 1 @08:50:45.36 Forearm.reconfigure TightForearm @08:50:45.39 Forearm.seek 1 + 800 counts @08:50:46.48 SE.reconfigure SEpick @08:50:46.53 SE.seek beamBreak @08:50:48.85 SE.configure SEconfig @08:50:48.97 Elbow.reconfigure LimpElbow @08:50:49.00 Forearm.reconfigure LooseForearm @08:50:49.04 Forearm.seek 1 @08:50:49.24 Elbow.hold @08:50:49.28 Elbow.reconfigure TightElbow @08:50:49.36 SE.seek 6.05 Pucks below Top Plate @08:50:49.71 Forearm.coast @08:50:49.74 Forearm.seek 1 + 347 counts @08:50:49.97 Forearm.reconfigure TightForearm @08:50:50.00 Forearm.seek 1 + 800 counts @08:50:50.26 SE.seek 7.05 Pucks below Top Plate @08:50:50.80 Forearm.reconfigure LooseForearm @08:50:50.83 Forearm.seek retract @08:50:51.78 Elbow.seek CC @08:50:53.32 Elbow.reconfigure LooseElbow @08:50:53.41 Forearm.seek CC @08:50:54.39 CC.seek sealed @08:50:55.32 Hand.grip @08:50:56.00 CC.seek open @08:50:59.35 Forearm.seek retract @08:51:00.28 Elbow.reconfigure TightElbow @08:51:00.32 Elbow.seek 1 @08:51:01.94 Elbow.reconfigure LimpElbow @08:51:01.98 Forearm.seek 1 @08:51:03.00 Elbow.hold @08:51:03.05 Elbow.reconfigure TightElbow @08:51:03.09 Hand.release @08:51:03.95 Forearm.coast @08:51:03.98 Forearm.seek 1 + 318 counts @08:51:04.20 Forearm.reconfigure TightForearm @08:51:04.23 Forearm.seek 1 + 800 counts @08:51:04.55 SE.seek down @08:51:05.66
Forearm.reconfigure LooseForearm @08:51:05.70 Forearm.seek retract @08:51:06.57 SE.coast @08:51:06.62
SC.coast @08:51:06.64 PSV.dial :air @08:51:07.70 PS.seek 0.499ml @08:51:13.55 delay 3 seconds @08:51:16.56 PSV.dial :mixloop @08:51:16.99 PRV.dial :air @08:51:17.44 delay 3 seconds @08:51:20.45 PRV.dial CBV @08:51:20.90 CBV.dial AIV @08:51:21.35 PRV.dial between CBV and air @08:51:21.66 PRV.dial :air @08:51:21.99 PS.reconfigure PSslow @08:51:22.02 PS.seek 0.599ml @08:51:24.62 PRV.dial :waste2 @08:51:25.34 PS.reconfigure PSconfig @08:51:25.37 PS.seek empty @08:51:32.33 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:51:32.47 Elbow.seek FlushPuck::Garage @08:51:34.14 Elbow.reconfigure LooseElbow @08:51:34.23 Forearm.seek garage @08:51:35.30 Hand.grip @08:51:36.08 Forearm.seek retract @08:51:37.11 Elbow.reconfigure TightElbow @08:51:37.15 Elbow.seek CC @08:51:39.92 Elbow.reconfigure LooseElbow @08:51:40.01 Forearm.reconfigure TightForearm @08:51:40.65 Forearm.seek CC @08:51:41.69 CC.seek closed @08:51:45.46 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.96 @08:51:45.51 Hand.release @08:51:46.29 Forearm.reconfigure LooseForearm @08:51:46.32 Forearm.seek retract @08:51:47.32 Clearing PML for modified lysate CTV.dial :air,avoiding => intake @08:51:47.33 PRV.dial :waste2 @08:51:47.34 PTV.dial :PRV,avoiding => puck @08:51:48.08 delay 2 seconds @08:51:50.08 PTV.dial :PRVmixing,avoiding => puck @08:51:50.82 delay 2 seconds @08:51:52.83 PSV.dial :PML @08:51:52.84 delay 2 seconds @08:51:54.84 PRV.dial :air @08:51:55.57 delay 2 seconds @08:51:57.63 PS.seek 1.499ml @08:52:13.71 delay 5 seconds @08:52:18.72 PS.seek 2.500ml @08:52:29.74 PSV.dial :air @08:52:30.19 PS.seek 0.000ml @08:52:56.42 mixing lysate with speDil @08:52:56.43 PSV.dial :PML @08:52:56.86 CBV.dial :PRV @08:52:56.87 PRV.dial :air @08:52:56.88 delay 2 seconds @08:52:58.89 PRV.dial :speDil @08:52:59.33 delay 2 seconds @08:53:01.39 PS.reconfigure PSslow1 @08:53:01.42 PS.seek 0.225ml @08:53:24.95 delay 5 seconds @08:53:29.96 PRV.dial CBV @08:53:30.69 delay 2 seconds @08:53:32.75 PS.seek 0.475ml @08:53:58.77 delay 5 seconds @08:54:03.78 PRV.dial :air @08:54:04.22 delay 2 seconds @08:54:06.23 PS.seek 0.925ml @08:54:52.33 delay 10 seconds @08:55:02.34 PRV.dial CBV @08:55:02.79 CTV.dial :air,avoiding => intake @08:55:02.80 PSV.dial :air @08:55:03.26 PS.reconfigure PSconfig @08:55:03.29 PS.seek 2.500ml @08:55:20.17 delay 3 seconds @08:55:23.20 PSV.dial PTV @08:55:23.91 PTV.dial :PRV,avoiding => puck @08:55:24.64 PS.seek 0.000ml @08:55:50.87 PSV.dial :air @08:55:51.59 PS.seek 2.500ml @08:56:17.83 delay 3 seconds @08:56:20.84 PSV.dial PTV @08:56:21.55 PTV.dial :PRV,avoiding => puck @08:56:21.57 PS.seek 0.000ml @08:56:47.83 Pushing modified lysate into AIV Injection Loop @08:56:47.84 CBV.dial AIV @08:56:47.85 AIV.dial :bypass @08:56:47.86 PRV.dial CBV @08:56:47.87 PSV.dial :air @08:56:48.59 PS.seek 2.500ml @08:57:14.80 delay 3 seconds @08:57:17.82 PSV.dial :PML @08:57:18.25 PTV.dial :PRVmixing,avoiding => puck @08:57:19.02 PS.reconfigure PSslow1 @08:57:19.05 PS.seek 1.850ml @08:58:25.48 delay 5 seconds @08:58:30.49 AIV.dial :sample @08:58:31.03 AWV.select :waste @08:58:31.19 PS.reconfigure PSslow2 @08:58:31.22 PS.seek 1.310ml @09:00:21.36 delay 15 seconds @09:00:36.37 AIV.dial :bypass @09:00:36.91 PS.reconfigure PSconfig @09:00:36.94 PS.seek 0.000ml @09:00:51.07 PSV.dial :air @09:00:51.52 PS.seek 2.500ml @09:01:17.79 PSV.dial :PML @09:01:18.24 PS.seek 0.000ml @09:01:44.45 AIV.dial :coilToMFB @09:01:44.46 AWV.select :air @09:01:44.60 Extracting Nucleic Acid from AIV @09:01:44.62
Beginning samplePassD @09:01:44.63 SPE.hold 2:46:40,55.0C @09:01:44.65 ASV.dial :SPEdelivery @09:01:44.78
AWV.select :air AIV.dial :bypass @09:01:44.79 PSV.dial PSR @09:01:45.20 delay 20 seconds @09:01:45.93
PSR.select :killMFB @09:01:46.03 PS.seek 1.500ml @09:02:02.10 delay 5 seconds @09:02:05.21 primeLysateINT @09:02:05.22 AWV.select :air @09:02:05.23 AIV.dial :coilToMFB ATV.dial :AIV @09:02:06.58 AS.reconfigure ASslow1 @09:02:06.62 AS.seek 0.239ml @09:02:07.11
PSV.dial :waste1 @09:02:08.13 PS.seek 0.000ml @09:02:24.23 PSV.dial PSR @09:02:24.68 PSR.select :air @09:02:24.78 PS.seek 2.500ml @09:02:51.01 PRV.dial CBV @09:02:51.03
CBV.dial PRV @09:02:51.04 PSV.dial :mixloop @09:02:52.07 CTV.dial :air,avoiding => intake @09:02:52.08 PTV.dial :PRVmixing @09:02:52.11 PS.seek 0.000ml @09:02:55.84 delay 10 seconds @09:03:05.85 ATV.dial :air2 @09:03:06.53 AS.seek 0.269ml @09:03:12.89 ATV.dial :waste2 @09:03:13.24 AS.reconfigure ASnorm @09:03:13.29 AS.seek empty @09:03:18.35
PSV.dial PSR @09:03:19.38 PSR.select :flush @09:03:19.48 PS.seek 1.500ml @09:03:27.47 Lysate primed! @09:03:27.48 AFV.select :holdingCoil @09:03:27.49 ATV.dial :air2 @09:03:27.89 AS.reconfigure ASslow1 @09:03:27.93 AS.seek 0.069ml @09:03:35.60
delay 5 seconds @09:03:40.61 PSV.dial :waste1 @09:03:41.06 PS.seek 0.000ml @09:03:42.53 delay 3 seconds @09:03:45.54 ATV.dial :AIV @09:03:46.13 AS.seek 0.469ml @09:03:57.22
PSV.dial PSR @09:03:57.67 PSR.select :air @09:03:57.77 PS.seek 2.500ml @09:04:23.97 PRV.dial CBV @09:04:23.99
CBV.dial PRV @09:04:24.00 PSV.dial :mixloop @09:04:25.02 CTV.dial :air,avoiding => intake @09:04:25.03 PTV.dial :PRVmixing @09:04:25.05 PS.seek 0.000ml @09:04:51.31 PSV.dial PSR @09:04:52.45 PSR.select :flush @09:04:52.55 PS.seek 1.500ml @09:05:08.00 delay 5 seconds @09:05:08.66
delay 5 seconds @09:05:13.05 Solid Phase Extraction Heater at 51.8C @09:05:13.06 ATV.dial ASV @09:05:13.40 ASV.dial :SPEdelivery @09:05:13.46 AS.seek 0.041ml @09:05:13.67
PSV.dial :waste1 @09:05:14.14 PS.seek 0.000ml @09:05:30.34 PSV.dial PSR @09:05:30.80 PSR.select :air @09:05:30.90 PS.seek 2.500ml @09:05:57.12 PRV.dial CBV @09:05:57.14
CBV.dial PRV @09:05:57.15 PSV.dial :mixloop @09:05:58.21 CTV.dial :air,avoiding => intake @09:05:58.22 PTV.dial :PRVmixing @09:05:58.24 PS.seek 0.000ml @09:06:24.62 PSV.dial PSR @09:06:25.64 PSR.select :flush @09:06:25.74 PS.seek 1.500ml @09:06:41.01 delay 10 seconds @09:06:41.84
delay 5 seconds @09:06:46.85 PSV.dial :waste1 @09:06:47.29 PS.seek 0.000ml @09:06:51.02 rinseLysateLineINT @09:06:51.03 AFV.select :carrierSolution @09:06:51.12 delay 2 seconds @09:06:53.13 AS.reconfigure ASconfig @09:06:53.16 AS.seek 1.000ml @09:07:03.37
PSV.dial PSR @09:07:03.81 PSR.select :air @09:07:03.91 PS.seek 2.500ml @09:07:30.12 PRV.dial CBV @09:07:30.14
CBV.dial PRV @09:07:30.15 PSV.dial :mixloop @09:07:31.15 CTV.dial :air,avoiding => intake @09:07:31.16 PTV.dial :PRVmixing @09:07:31.17 PS.reconfigure PSslow @09:07:31.21 PS.seek 1.200ml @09:07:49.35 delay 1 second @09:07:50.36 AFV.select :holdingCoil @09:07:50.45 delay 1 second @09:07:51.46 ATV.dial :AIV @09:07:51.80 AIV.dial :coilToMFB @09:07:51.81 AWV.select :waste @09:07:51.90 AS.reconfigure ASslow0 @09:07:51.94 AS.seek 0.000ml @09:07:58.21
PSV.dial :waste1 @09:07:59.52 PS.reconfigure PSconfig @09:07:59.55 PS.seek 0.000ml @09:08:12.65 PSV.dial :air @09:08:13.68 PS.seek 2.500ml @09:08:39.87 PSV.dial :mixloop @09:08:40.32 PS.reconfigure PSslow @09:08:40.35 PS.seek 0.000ml @09:09:31.68 PSV.dial :air @09:09:32.13 PS.reconfigure PSconfig @09:09:32.16 PS.seek 2.500ml @09:09:34.25 ATV.dial :air2 @09:09:34.83 AS.reconfigure ASslow1 @09:09:34.86 AS.seek 0.050ml @09:09:45.35 ATV.dial :AIV @09:09:45.93 AS.seek 0.010ml @09:09:54.33 ATV.dial :waste @09:09:55.62 AS.seek empty @09:09:58.03 ethanol wash @09:09:58.04 ATV.dial :air2 @09:09:58.43
PSV.dial :mixloop @09:09:59.05 AS.reconfigure ASslow2 @09:09:59.08 AS.seek 0.009ml @09:09:59.41
PS.reconfigure PSslow @09:09:59.44 PS.seek 0.000ml @09:10:09.52 delay 3 seconds @09:10:12.53 Pulling 0.4ml of Ethanol Wash @09:10:12.54 ATV.dial :ethanol @09:10:13.19 AS.reconfigure ASslow1 @09:10:13.22 AS.seek 0.409ml @09:10:50.74
Cleaning Collection Syringe @09:10:50.75 CSV.dial CSR @09:10:51.18 CSR.select :flush @09:10:51.28 CS.seek 4.000ml @09:11:02.31 delay 5 seconds @09:11:07.32 CSR.select :air @09:11:07.41 CS.seek 10.000ml @09:11:23.43 CSV.dial CTV @09:11:23.45
CTV.dial CSV @09:11:24.77 CBV.dial :waste1 @09:11:25.25 CS.seek 0.000ml @09:11:35.06 delay 3 seconds @09:11:38.07 ATV.dial :air1 @09:11:38.48 delay 3 seconds @09:11:41.54 AS.seek 0.434ml @09:11:46.89 delay 3 seconds @09:11:49.90 ATV.dial :ethanol @09:11:50.30 AS.seek 0.419ml @09:11:51.40
CSV.dial CSR @09:11:51.90 CSR.select :flush @09:11:52.00 CS.seek 4.000ml @09:11:53.62 delay 3 seconds @09:11:56.63 ATV.dial ASV @09:11:57.86 AS.seek 0.092ml @09:12:02.93
delay 5 seconds @09:12:07.94 CSR.select :air @09:12:08.03 CS.seek 10.000ml @09:12:23.99 CSV.dial CTV @09:12:24.01
CTV.dial CSV @09:12:24.52 CBV.dial :waste1 @09:12:24.54 CS.seek 0.000ml @09:12:50.71 Garaging FlushPuck in Collection Clamp @09:12:50.76 Elbow.reconfigure TightElbow @09:12:50.79 Elbow.seek CC @09:12:50.89 Elbow.reconfigure LooseElbow @09:12:50.97 Forearm.seek CC @09:12:51.93 CC.seek sealed @09:12:52.87 Hand.grip @09:12:53.59 CC.seek open @09:12:56.91 Forearm.seek retract @09:12:57.84 Elbow.reconfigure TightElbow @09:12:57.88 Elbow.seek garage @09:13:00.72 Elbow.reconfigure LooseElbow @09:13:00.80 Forearm.reconfigure TightForearm @09:13:00.83 Forearm.seek garage @09:13:01.98 Hand.release @09:13:02.75 Forearm.reconfigure LooseForearm @09:13:02.79 Forearm.seek retract @09:13:03.83 Whole Cell Archive sampling 1000ml @09:13:03.85 Loading wcr puck from tube 2 into Collection Clamp @09:13:04.02 SC.reconfigure SCtight @09:13:04.06 SC.seek 2 @09:13:04.80 delay 3 seconds @09:13:06.38 SC.reconfigure SCloose @09:13:06.56 Elbow.reconfigure TightElbow @09:13:06.59 Elbow.seek 2 @09:13:07.81 ATV.dial :waste2 @09:13:08.34 AS.reconfigure ASconfig @09:13:08.39 AS.seek empty @09:13:08.52 Forearm.reconfigure TightForearm @09:13:08.56 Forearm.seek 2 + 800 counts @09:13:09.66 SE.reconfigure SEpick @09:13:09.72 SE.seek beamBreak @09:13:11.39 SE.configure SEconfig @09:13:11.51 Elbow.seek 2 @09:13:11.66 Forearm.seek 2 + 300 counts @09:13:11.92 Forearm.reconfigure LooseForearm @09:13:11.96 Hand.grip @09:13:12.72 SE.seek down @09:13:12.87 Forearm.seek retract @09:13:13.85 Elbow.seek CC @09:13:14.13 ATV.dial :air2 @09:13:14.24 SE.coast @09:13:14.47 AS.reconfigure ASslow1 @09:13:15.09 AS.seek 1.000ml @09:13:15.37 Elbow.reconfigure LooseElbow @09:13:15.46 Forearm.reconfigure TightForearm @09:13:15.49 Forearm.seek CC @09:13:16.63 CC.seek closed @09:13:20.43 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.93 @09:13:20.44 Hand.release @09:13:21.25 Forearm.reconfigure LooseForearm @09:13:21.28 Forearm.seek retract @09:13:22.25 SC.coast @09:13:22.28 collectSample 1000ml w/bubble point of 55psi @09:13:22.30 Priming 7ml Sleepy.power :sampler,:ON @09:13:22.31 Intake.select :close @09:13:22.32 Exhaust.select :close @09:13:22.33 SS.hold @09:13:22.49 Calibrating pressure sensors @09:13:22.50 CTV.dial :air @09:13:23.80 SSV.dial :spit,avoiding => exhaust @09:13:23.81 SS.reconfigure SSfast @09:13:23.84 SS.seek 5.00ml @09:13:26.87 SSV.dial CBV @09:13:26.89 CBV.dial SSV @09:13:28.29 delay 3.8 seconds @09:13:32.19 SSV.dial :spit @09:13:33.55 SS.seek 0.00ml @09:13:35.95 SSV.dial CBV @09:13:37.29 CTV.dial :intake @09:13:38.01 Waiting for external sampler to finish priming... @09:13:38.02 delayUntil "09:23:22.30" @09:16:39.48 delay 5 seconds @09:16:44.49 ATV.dial ASV @09:16:45.20 ASV.dial :SPEdelivery @09:16:45.22 AS.seek 0.020ml @09:20:05.40 delay 5 seconds @09:20:10.41 ATV.dial :air2 @09:20:11.18 AS.seek 0.520ml @09:21:53.44 delay 5 seconds @09:21:58.45 ATV.dial ASV @09:21:59.16 ASV.dial :wasteR @09:21:59.76 AS.seek 0.300ml @09:22:44.88 delay 20 seconds @09:23:04.89 AS.seek 0.070ml @09:23:22.30 SSV.dial CBV @09:23:22.32 CBV.dial SSV @09:23:22.33 Allowing 55s for 7.0ml of remaining 7.0ml @09:23:22.34 Intake.select :open @09:23:24.47 Intake @44.2psia, vacuum from 20.0 to 24.0psi @09:23:24.49 SS.reconfigure forSampling @09:23:24.52 SS.seek 7.00ml @09:23:30.20 Stroke completed -- waiting for pressure to equalize ... @09:23:30.84 Intake.select :close @09:23:30.97 SSV.dial :exhaust @09:23:31.76 Exhaust.select :open @09:23:31.86 SS.reconfigure SSexhale @09:23:31.90 SS.seek 0.00ml @09:23:35.25 Exhaust.select :close @09:23:35.36 Sampling 1000.0ml @09:23:35.37 Intake.select :close @09:23:35.38 Exhaust.select :close @09:23:35.39 SS.reconfigure SSconfig @09:23:35.42 SS.hold @09:23:35.57 CTV.dial :intake @09:23:35.58 SSV.dial CBV @09:23:35.60 CBV.dial SSV @09:23:36.40 Allowing 185s for 25.0ml of remaining 1000.0ml @09:23:36.41 Intake.select :open @09:23:38.54 Intake @42.4psia, vacuum from 20.0 to 24.0psi @09:23:38.56 SS.reconfigure forSampling @09:23:38.59 SS.seek 25.00ml @09:23:52.05 delay 20 seconds @09:23:53.03 Stroke completed -- waiting for pressure to equalize ... @09:23:53.67 Intake.select :close @09:23:53.81 SSV.dial :exhaust @09:23:54.59 Exhaust.select :open @09:23:54.69 SS.reconfigure SSexhale @09:23:54.73 SS.seek 0.00ml @09:24:03.51 Exhaust.select :close @09:24:03.61 SSV.dial CBV @09:24:03.63 CBV.dial SSV @09:24:04.43 Allowing 185s for 25.0ml of remaining 975.0ml @09:24:04.44 Intake.select :open @09:24:06.58 Intake @41.9psia, vacuum from 20.0 to 24.0psi @09:24:06.60 SS.reconfigure forSampling @09:24:06.63 SS.seek 25.00ml @09:24:12.06 ATV.dial :waste2 @09:24:12.60 AS.reconfigure ASconfig @09:24:12.63 AS.seek empty @09:24:16.98 Vacuum too high -- waiting up to 40s @09:24:17.05 Elution @09:24:17.06 ATV.dial :air1 @09:24:17.25 Intake @35.6psia, vacuum from 20.0 to 24.0psi @09:24:17.26 SS.reconfigure forSampling @09:24:17.30 SS.seek 25.00ml @09:24:18.04 AS.reconfigure ASslow1 @09:24:18.07 AS.seek 0.049ml @09:24:22.06 Stroke completed -- waiting for pressure to equalize ... @09:24:23.24 Intake.select :close @09:24:23.38 SSV.dial :exhaust @09:24:24.17 Exhaust.select :open @09:24:24.27 SS.reconfigure SSexhale @09:24:24.31 SS.seek 0.00ml @09:24:28.56 delay 3 seconds @09:24:31.57 ATV.dial :ElutionWater @09:24:32.14 AS.seek 0.109ml @09:24:33.32 Exhaust.select :close @09:24:33.42 SSV.dial CBV @09:24:33.44 CBV.dial SSV @09:24:34.25 Allowing 185s for 25.0ml of remaining 950.0ml @09:24:34.26 Intake.select :open @09:24:36.39 Intake @44.0psia, vacuum from 20.0 to 24.0psi @09:24:36.41 SS.reconfigure forSampling @09:24:36.44 SS.seek 25.00ml @09:24:39.98 Vacuum too high -- waiting up to 40s @09:24:40.24 Intake @38.0psia, vacuum from 20.0 to 24.0psi @09:24:40.25 SS.reconfigure forSampling @09:24:40.29 SS.seek 25.00ml @09:24:44.64 delay 3 seconds @09:24:47.65 ATV.dial ASV,via => air2 @09:24:48.19 Vacuum too high -- waiting up to 40s @09:24:48.68 Intake @40.0psia, vacuum from 20.0 to 24.0psi @09:24:48.69 SS.reconfigure forSampling @09:24:48.73 SS.seek 25.00ml @09:24:49.34 ASV.dial :SPEelution @09:24:49.86 AFV.select :carrierSolution @09:24:50.01 AS.reconfigure ASnorm @09:24:50.04 AS.seek 0.759ml @09:24:51.73 Vacuum too high -- waiting up to 40s @09:24:52.22 Intake @41.8psia, vacuum from 20.0 to 24.0psi @09:24:52.23 SS.reconfigure forSampling @09:24:52.27 SS.seek 25.00ml @09:24:55.01 Vacuum too high -- waiting up to 40s @09:24:55.50 Intake @42.7psia, vacuum from 20.0 to 24.0psi @09:24:55.51 SS.reconfigure forSampling @09:24:55.55 SS.seek 25.00ml @09:24:58.29 Stroke completed -- waiting for pressure to equalize ... @09:24:58.93 Intake.select :close @09:24:59.07 SSV.dial :exhaust @09:24:59.85 Exhaust.select :open @09:24:59.95 SS.reconfigure SSexhale @09:24:59.99 SS.seek 0.00ml @09:25:08.98 Exhaust.select :close @09:25:09.08 SSV.dial CBV @09:25:09.10 CBV.dial SSV @09:25:09.88 Allowing 185s for 25.0ml of remaining 925.0ml @09:25:09.89 Intake.select :open @09:25:12.03 Intake @44.5psia, vacuum from 20.0 to 24.0psi @09:25:12.05 SS.reconfigure forSampling @09:25:12.08 SS.seek 25.00ml @09:25:14.26 Vacuum too high -- waiting up to 40s @09:25:14.53 Intake @41.7psia, vacuum from 20.0 to 24.0psi @09:25:14.54 SS.reconfigure forSampling @09:25:14.58 SS.seek 25.00ml @09:25:23.53 delay 3 seconds @09:25:25.79 Vacuum too high -- waiting up to 40s @09:25:26.28 Intake @43.6psia, vacuum from 20.0 to 24.0psi @09:25:26.29 SS.reconfigure forSampling @09:25:26.33 SS.seek 25.00ml @09:25:26.54 AFV.select :holdingCoil @09:25:26.68 AS.reconfigure ASslow1 @09:25:26.71 AS.seek 0.489ml @09:25:37.97 Vacuum too high -- waiting up to 40s @09:25:38.24 Intake @43.2psia, vacuum from 20.0 to 24.0psi @09:25:38.25 SS.reconfigure forSampling @09:25:38.29 SS.seek 25.00ml @09:25:55.15 Stroke completed -- waiting for pressure to equalize ... @09:25:56.38 Intake.select :close @09:25:56.51 SSV.dial :exhaust @09:25:57.30 Exhaust.select :open @09:25:57.40 SS.reconfigure SSexhale @09:25:57.44 SS.seek 0.00ml @09:26:06.46 Exhaust.select :close @09:26:06.56 SSV.dial CBV @09:26:06.58 CBV.dial SSV @09:26:07.38 Allowing 185s for 25.0ml of remaining 900.0ml @09:26:07.39 Intake.select :open @09:26:09.53 Intake @44.3psia, vacuum from 20.0 to 24.0psi @09:26:09.55 SS.reconfigure forSampling @09:26:09.58 SS.seek 25.00ml @09:26:22.03 delay 5 seconds @09:26:27.09 AS.reconfigure ASslow2 @09:26:27.12 AS.seek 0.454ml @09:26:29.38 Vacuum too high -- waiting up to 40s @09:26:29.65 Intake @42.8psia, vacuum from 20.0 to 24.0psi @09:26:29.66 SS.reconfigure forSampling @09:26:29.69 SS.seek 25.00ml @09:27:02.92 delay 1:00 @09:27:43.45 Stroke completed -- waiting for pressure to equalize ... @09:27:44.68 Intake.select :close @09:27:44.82 SSV.dial :exhaust @09:27:46.19 Exhaust.select :open @09:27:46.29 SS.reconfigure SSexhale @09:27:46.33 SS.seek 0.00ml @09:27:55.55 Exhaust.select :close @09:27:55.65 SSV.dial CBV @09:27:55.67 CBV.dial SSV @09:27:56.48 Allowing 185s for 25.0ml of remaining 875.0ml @09:27:56.49 Intake.select :open @09:27:58.63 Intake @43.8psia, vacuum from 20.0 to 24.0psi @09:27:58.65 SS.reconfigure forSampling @09:27:58.68 SS.seek 25.00ml @09:28:02.93 pushing eluate to holding coil @09:28:02.99 AS.seek 0.294ml @09:29:46.75 Vacuum too high -- waiting up to 40s @09:29:47.24 Intake @43.1psia, vacuum from 20.0 to 24.0psi @09:29:47.26 SS.reconfigure forSampling @09:29:47.29 SS.seek 25.00ml @09:29:49.74 Stroke completed -- waiting for pressure to equalize ... @09:29:52.14 Intake.select :close @09:29:52.28 SSV.dial :exhaust @09:29:53.08 Exhaust.select :open @09:29:53.18 SS.reconfigure SSexhale @09:29:53.22 SS.seek 0.00ml @09:30:01.94 Exhaust.select :close @09:30:02.04 SSV.dial CBV @09:30:02.06 CBV.dial SSV @09:30:02.86 Allowing 185s for 25.0ml of remaining 850.0ml @09:30:02.88 Intake.select :open @09:30:05.01 Intake @43.5psia, vacuum from 20.0 to 24.0psi @09:30:05.03 SS.reconfigure forSampling @09:30:05.06 SS.seek 25.00ml @09:30:14.06 Vacuum too high -- waiting up to 40s @09:30:14.55 Intake @43.1psia, vacuum from 20.0 to 24.0psi @09:30:14.56 SS.reconfigure forSampling @09:30:14.60 SS.seek 25.00ml @09:30:22.47 Vacuum too high -- waiting up to 40s @09:30:22.95 Intake @43.3psia, vacuum from 20.0 to 24.0psi @09:30:22.96 SS.reconfigure forSampling @09:30:23.00 SS.seek 25.00ml @09:30:26.25 Vacuum too high -- waiting up to 40s @09:30:26.73 Intake @42.9psia, vacuum from 20.0 to 24.0psi @09:30:26.74 SS.reconfigure forSampling @09:30:26.78 SS.seek 25.00ml @09:30:30.31 Vacuum too high -- waiting up to 40s @09:30:30.79 Intake @43.3psia, vacuum from 20.0 to 24.0psi @09:30:30.80 SS.reconfigure forSampling @09:30:30.83 SS.seek 25.00ml @09:30:34.19 Vacuum too high -- waiting up to 40s @09:30:34.67 Intake @43.5psia, vacuum from 20.0 to 24.0psi @09:30:34.68 SS.reconfigure forSampling @09:30:34.72 SS.seek 25.00ml @09:30:37.38 Vacuum too high -- waiting up to 40s @09:30:37.87 Intake @43.4psia, vacuum from 20.0 to 24.0psi @09:30:37.89 SS.reconfigure forSampling @09:30:37.92 SS.seek 25.00ml @09:30:40.35 Vacuum too high -- waiting up to 40s @09:30:40.82 Intake @43.0psia, vacuum from 20.0 to 24.0psi @09:30:40.83 SS.reconfigure forSampling @09:30:40.87 SS.seek 25.00ml @09:30:43.66 Vacuum too high -- waiting up to 40s @09:30:44.14 Intake @43.0psia, vacuum from 20.0 to 24.0psi @09:30:44.15 SS.reconfigure forSampling @09:30:44.19 SS.seek 25.00ml @09:30:46.53 delay 30 seconds @09:30:47.31 Vacuum too high -- waiting up to 40s @09:30:48.04 Intake @43.9psia, vacuum from 20.0 to 24.0psi @09:30:48.05 SS.reconfigure forSampling @09:30:48.08 SS.seek 25.00ml @09:30:50.32 Vacuum too high -- waiting up to 40s @09:30:50.79 Intake @43.0psia, vacuum from 20.0 to 24.0psi @09:30:50.80 SS.reconfigure forSampling @09:30:50.84 SS.seek 25.00ml @09:30:53.38 Vacuum too high -- waiting up to 40s @09:30:53.86 Intake @43.4psia, vacuum from 20.0 to 24.0psi @09:30:53.87 SS.reconfigure forSampling @09:30:53.91 SS.seek 25.00ml @09:30:56.07 Vacuum too high -- waiting up to 40s @09:30:57.19 Intake @43.5psia, vacuum from 20.0 to 24.0psi @09:30:57.21 SS.reconfigure forSampling @09:30:57.24 SS.seek 25.00ml @09:31:00.04 Vacuum too high -- waiting up to 40s @09:31:00.53 Intake @42.4psia, vacuum from 20.0 to 24.0psi @09:31:00.54 SS.reconfigure forSampling @09:31:00.58 SS.seek 25.00ml @09:31:04.93 Vacuum too high -- waiting up to 40s @09:31:05.64 Intake @43.8psia, vacuum from 20.0 to 24.0psi @09:31:05.66 SS.reconfigure forSampling @09:31:05.69 SS.seek 25.00ml @09:31:07.69 Vacuum too high -- waiting up to 40s @09:31:08.18 Intake @42.7psia, vacuum from 20.0 to 24.0psi @09:31:08.20 SS.reconfigure forSampling @09:31:08.23 SS.seek 25.00ml @09:31:11.28 Vacuum too high -- waiting up to 40s @09:31:11.98 Intake @44.3psia, vacuum from 20.0 to 24.0psi @09:31:12.00 SS.reconfigure forSampling @09:31:12.03 SS.seek 25.00ml @09:31:13.69 Vacuum too high -- waiting up to 40s @09:31:14.18 Intake @43.1psia, vacuum from 20.0 to 24.0psi @09:31:14.20 SS.reconfigure forSampling @09:31:14.23 SS.seek 25.00ml @09:31:16.54 ATV.dial :waste,via => air3 @09:31:17.10 Vacuum too high -- waiting up to 40s @09:31:17.79 Intake @43.0psia, vacuum from 20.0 to 24.0psi @09:31:17.81 SS.reconfigure forSampling @09:31:17.84 SS.seek 25.00ml @09:31:18.15 AS.reconfigure ASconfig @09:31:18.18 AS.seek empty @09:31:20.54 Vacuum too high -- waiting up to 40s @09:31:21.25 Intake @43.5psia, vacuum from 20.0 to 24.0psi @09:31:21.26 SS.reconfigure forSampling @09:31:21.30 SS.seek 25.00ml @09:31:23.88 Vacuum too high -- waiting up to 40s @09:31:24.57 Intake @43.3psia, vacuum from 20.0 to 24.0psi @09:31:24.58 SS.reconfigure forSampling @09:31:24.61 SS.seek 25.00ml @09:31:27.00 Vacuum too high -- waiting up to 40s @09:31:27.71 Intake @43.8psia, vacuum from 20.0 to 24.0psi @09:31:27.72 SS.reconfigure forSampling @09:31:27.75 SS.seek 25.00ml @09:31:29.63 Vacuum too high -- waiting up to 40s @09:31:30.11 Intake @43.8psia, vacuum from 20.0 to 24.0psi @09:31:30.12 SS.reconfigure forSampling @09:31:30.16 SS.seek 25.00ml @09:31:32.00 Vacuum too high -- waiting up to 40s @09:31:32.48 SS.seek 25.00ml @09:31:34.38 Vacuum too high -- waiting up to 40s @09:31:35.08 Intake @44.3psia, vacuum from 20.0 to 24.0psi @09:31:35.10 SS.reconfigure forSampling @09:31:35.13 SS.seek 25.00ml @09:31:35.64 Parking Bleach in LysateSource line and SPEcolumn @09:31:35.65 ATV.dial :air3 @09:31:36.23 AS.reconfigure ASslow1 @09:31:36.26 AS.seek 0.029ml @09:31:36.94 Vacuum too high -- waiting up to 40s @09:31:37.43 Intake @43.9psia, vacuum from 20.0 to 24.0psi @09:31:37.44 SS.reconfigure forSampling @09:31:37.48 SS.seek 25.00ml @09:31:39.38 Vacuum too high -- waiting up to 40s @09:31:40.07 Intake @43.4psia, vacuum from 20.0 to 24.0psi @09:31:40.09 SS.reconfigure forSampling @09:31:40.12 SS.seek 25.00ml @09:31:42.28 Vacuum too high -- waiting up to 40s @09:31:42.72 ATV.dial :ElutionWater @09:31:43.02 Intake @43.2psia, vacuum from 20.0 to 24.0psi @09:31:43.04 SS.reconfigure forSampling @09:31:43.71 SS.seek 25.00ml @09:31:43.81 AS.seek 0.059ml @09:31:46.84 Vacuum too high -- waiting up to 40s @09:31:47.55 Intake @42.6psia, vacuum from 20.0 to 24.0psi @09:31:47.57 SS.reconfigure forSampling @09:31:47.60 SS.seek 25.00ml @09:31:49.97 Vacuum too high -- waiting up to 40s @09:31:50.16 ATV.dial :air3 @09:31:50.56 AS.seek 0.069ml @09:31:50.73 Intake @42.2psia, vacuum from 20.0 to 24.0psi @09:31:50.75 SS.reconfigure forSampling @09:31:50.78 SS.seek 25.00ml @09:31:52.87 delay 2 seconds @09:31:53.16 Vacuum too high -- waiting up to 40s @09:31:53.87 Intake @41.8psia, vacuum from 20.0 to 24.0psi @09:31:53.89 SS.reconfigure forSampling @09:31:53.92 SS.seek 25.00ml @09:31:54.88 ATV.dial :bleach @09:31:55.28 AS.seek 0.709ml @09:31:56.32 Vacuum too high -- waiting up to 40s @09:31:57.04 Intake @42.0psia, vacuum from 20.0 to 24.0psi @09:31:57.06 SS.reconfigure forSampling @09:31:57.09 SS.seek 25.00ml @09:31:59.44 Vacuum too high -- waiting up to 40s @09:32:00.16 SS.seek 25.00ml @09:32:02.47 Vacuum too high -- waiting up to 40s @09:32:03.16 Intake @41.7psia, vacuum from 20.0 to 24.0psi @09:32:03.18 SS.reconfigure forSampling @09:32:03.21 SS.seek 25.00ml @09:32:05.66 Vacuum too high -- waiting up to 40s @09:32:06.58 Intake @42.3psia, vacuum from 20.0 to 24.0psi @09:32:06.59 SS.reconfigure forSampling @09:32:06.62 SS.seek 25.00ml @09:32:08.91 Vacuum too high -- waiting up to 40s @09:32:09.61 Intake @42.1psia, vacuum from 20.0 to 24.0psi @09:32:09.63 SS.reconfigure forSampling @09:32:09.66 SS.seek 25.00ml @09:32:11.78 Vacuum too high -- waiting up to 40s @09:32:12.48 Intake @41.5psia, vacuum from 20.0 to 24.0psi @09:32:12.49 SS.reconfigure forSampling @09:32:12.53 SS.seek 25.00ml @09:32:14.75 Vacuum too high -- waiting up to 40s @09:32:15.66 Intake @41.9psia, vacuum from 20.0 to 24.0psi @09:32:15.67 SS.reconfigure forSampling @09:32:15.71 SS.seek 25.00ml @09:32:18.25 Vacuum too high -- waiting up to 40s @09:32:19.16 Intake @42.6psia, vacuum from 20.0 to 24.0psi @09:32:19.17 SS.reconfigure forSampling @09:32:19.21 SS.seek 25.00ml @09:32:21.31 Vacuum too high -- waiting up to 40s @09:32:22.00 Intake @41.5psia, vacuum from 20.0 to 24.0psi @09:32:22.01 SS.reconfigure forSampling @09:32:22.05 SS.seek 25.00ml @09:32:24.19 Vacuum too high -- waiting up to 40s @09:32:25.09 Intake @42.0psia, vacuum from 20.0 to 24.0psi @09:32:25.10 SS.reconfigure forSampling @09:32:25.14 SS.seek 25.00ml @09:32:27.41 Vacuum too high -- waiting up to 40s @09:32:28.77 Intake @41.7psia, vacuum from 20.0 to 24.0psi @09:32:28.79 SS.reconfigure forSampling @09:32:28.82 SS.seek 25.00ml @09:32:31.34 Vacuum too high -- waiting up to 40s @09:32:32.27 Intake @42.2psia, vacuum from 20.0 to 24.0psi @09:32:32.29 SS.reconfigure forSampling @09:32:32.32 SS.seek 25.00ml @09:32:34.53 Vacuum too high -- waiting up to 40s @09:32:35.24 Intake @41.7psia, vacuum from 20.0 to 24.0psi @09:32:35.26 SS.reconfigure forSampling @09:32:35.29 SS.seek 25.00ml @09:32:35.60 Stroke completed -- waiting for pressure to equalize ... @09:32:38.02 Intake.select :close @09:32:38.15 SSV.dial :exhaust @09:32:38.95 Exhaust.select :open @09:32:39.05 SS.reconfigure SSexhale @09:32:39.09 SS.seek 0.00ml @09:32:47.92 Exhaust.select :close @09:32:48.01 SSV.dial CBV @09:32:48.03 CBV.dial SSV @09:32:48.84 Allowing 185s for 25.0ml of remaining 825.0ml @09:32:48.85 Intake.select :open @09:32:50.99 Intake @41.9psia, vacuum from 20.0 to 24.0psi @09:32:51.01 SS.reconfigure forSampling @09:32:51.04 SS.seek 25.00ml @09:32:54.06 Vacuum too high -- waiting up to 40s @09:32:54.97 Intake @41.2psia, vacuum from 20.0 to 24.0psi @09:32:54.98 SS.reconfigure forSampling @09:32:55.01 SS.seek 25.00ml @09:32:57.56 Vacuum too high -- waiting up to 40s @09:32:58.47 Intake @41.5psia, vacuum from 20.0 to 24.0psi @09:32:58.48 SS.reconfigure forSampling @09:32:58.52 SS.seek 25.00ml @09:33:01.16 Vacuum too high -- waiting up to 40s @09:33:02.07 Intake @42.1psia, vacuum from 20.0 to 24.0psi @09:33:02.08 SS.reconfigure forSampling @09:33:02.12 SS.seek 25.00ml @09:33:04.16 Vacuum too high -- waiting up to 40s @09:33:04.84 Intake @41.5psia, vacuum from 20.0 to 24.0psi @09:33:04.85 SS.reconfigure forSampling @09:33:04.89 SS.seek 25.00ml @09:33:06.84 Vacuum too high -- waiting up to 40s @09:33:07.76 Intake @41.7psia, vacuum from 20.0 to 24.0psi @09:33:07.77 SS.reconfigure forSampling @09:33:07.81 SS.seek 25.00ml @09:33:10.03 Vacuum too high -- waiting up to 40s @09:33:10.93 Intake @42.2psia, vacuum from 20.0 to 24.0psi @09:33:10.94 SS.reconfigure forSampling @09:33:10.98 SS.seek 25.00ml @09:33:12.97 Vacuum too high -- waiting up to 40s @09:33:13.68 Intake @41.6psia, vacuum from 20.0 to 24.0psi @09:33:13.70 SS.reconfigure forSampling @09:33:13.73 SS.seek 25.00ml @09:33:15.72 Vacuum too high -- waiting up to 40s @09:33:16.63 Intake @42.3psia, vacuum from 20.0 to 24.0psi @09:33:16.64 SS.reconfigure forSampling @09:33:16.68 SS.seek 25.00ml @09:33:18.69 Vacuum too high -- waiting up to 40s @09:33:19.39 SS.seek 25.00ml @09:33:20.97 Vacuum too high -- waiting up to 40s @09:33:21.89 Intake @41.8psia, vacuum from 20.0 to 24.0psi @09:33:21.91 SS.reconfigure forSampling @09:33:21.94 SS.seek 25.00ml @09:33:24.15 Vacuum too high -- waiting up to 40s @09:33:25.08 Intake @42.0psia, vacuum from 20.0 to 24.0psi @09:33:25.10 SS.reconfigure forSampling @09:33:25.13 SS.seek 25.00ml @09:33:27.28 Vacuum too high -- waiting up to 40s @09:33:28.22 Intake @41.7psia, vacuum from 20.0 to 24.0psi @09:33:28.23 SS.reconfigure forSampling @09:33:28.26 SS.seek 25.00ml @09:33:30.31 Vacuum too high -- waiting up to 40s @09:33:31.23 Intake @42.7psia, vacuum from 20.0 to 24.0psi @09:33:31.24 SS.reconfigure forSampling @09:33:31.28 SS.seek 25.00ml @09:33:33.37 Vacuum too high -- waiting up to 40s @09:33:34.08 Intake @42.3psia, vacuum from 20.0 to 24.0psi @09:33:34.09 SS.reconfigure forSampling @09:33:34.13 SS.seek 25.00ml @09:33:35.93 Vacuum too high -- waiting up to 40s @09:33:36.86 Intake @42.7psia, vacuum from 20.0 to 24.0psi @09:33:36.87 SS.reconfigure forSampling @09:33:36.91 SS.seek 25.00ml @09:33:38.68 Vacuum too high -- waiting up to 40s @09:33:39.39 Intake @41.7psia, vacuum from 20.0 to 24.0psi @09:33:39.41 SS.reconfigure forSampling @09:33:39.44 SS.seek 25.00ml @09:33:41.25 Vacuum too high -- waiting up to 40s @09:33:42.16 Intake @42.3psia, vacuum from 20.0 to 24.0psi @09:33:42.18 SS.reconfigure forSampling @09:33:42.21 SS.seek 25.00ml @09:33:44.15 Vacuum too high -- waiting up to 40s @09:33:45.07 Intake @42.3psia, vacuum from 20.0 to 24.0psi @09:33:45.09 SS.reconfigure forSampling @09:33:45.12 SS.seek 25.00ml @09:33:47.22 Vacuum too high -- waiting up to 40s @09:33:48.15 Intake @42.7psia, vacuum from 20.0 to 24.0psi @09:33:48.16 SS.reconfigure forSampling @09:33:48.20 SS.seek 25.00ml @09:33:50.03 Vacuum too high -- waiting up to 40s @09:33:50.71 Intake @42.3psia, vacuum from 20.0 to 24.0psi @09:33:50.72 SS.reconfigure forSampling @09:33:50.76 SS.seek 25.00ml @09:33:52.31 Vacuum too high -- waiting up to 40s @09:33:53.22 Intake @42.0psia, vacuum from 20.0 to 24.0psi @09:33:53.23 SS.reconfigure forSampling @09:33:53.27 SS.seek 25.00ml @09:33:55.25 Vacuum too high -- waiting up to 40s @09:33:56.14 Intake @41.8psia, vacuum from 20.0 to 24.0psi @09:33:56.16 SS.reconfigure forSampling @09:33:56.19 SS.seek 25.00ml @09:33:58.31 Vacuum too high -- waiting up to 40s @09:33:59.21 Intake @42.2psia, vacuum from 20.0 to 24.0psi @09:33:59.22 SS.reconfigure forSampling @09:33:59.25 SS.seek 25.00ml @09:34:02.28 Vacuum too high -- waiting up to 40s @09:34:03.20 Intake @43.2psia, vacuum from 20.0 to 24.0psi @09:34:03.22 SS.reconfigure forSampling @09:34:03.25 SS.seek 25.00ml @09:34:04.90 Vacuum too high -- waiting up to 40s @09:34:05.61 Intake @42.2psia, vacuum from 20.0 to 24.0psi @09:34:05.63 SS.reconfigure forSampling @09:34:05.66 SS.seek 25.00ml @09:34:06.07 delay 3 seconds @09:34:07.43 Vacuum too high -- waiting up to 40s @09:34:08.37 Intake @42.5psia, vacuum from 20.0 to 24.0psi @09:34:08.38 SS.reconfigure forSampling @09:34:08.41 SS.seek 25.00ml @09:34:09.08 ATV.dial :air3 @09:34:09.46 delay 3 seconds @09:34:10.18 Vacuum too high -- waiting up to 40s @09:34:11.11 Intake @42.3psia, vacuum from 20.0 to 24.0psi @09:34:11.13 SS.reconfigure forSampling @09:34:11.16 SS.seek 25.00ml @09:34:12.51 AS.seek 0.734ml @09:34:12.75 Vacuum too high -- waiting up to 40s @09:34:13.67 Intake @42.0psia, vacuum from 20.0 to 24.0psi @09:34:13.69 SS.reconfigure forSampling @09:34:13.72 SS.seek 25.00ml @09:34:15.53 Vacuum too high -- waiting up to 40s @09:34:16.45 Intake @41.9psia, vacuum from 20.0 to 24.0psi @09:34:16.46 SS.reconfigure forSampling @09:34:16.50 SS.seek 25.00ml @09:34:17.85 delay 3 seconds @09:34:18.53 Vacuum too high -- waiting up to 40s @09:34:19.46 Intake @43.2psia, vacuum from 20.0 to 24.0psi @09:34:19.48 SS.reconfigure forSampling @09:34:19.51 SS.seek 25.00ml @09:34:20.86 ATV.dial :bleach @09:34:21.15 Vacuum too high -- waiting up to 40s @09:34:21.28 AS.seek 0.719ml @09:34:21.88 Intake @42.5psia, vacuum from 20.0 to 24.0psi @09:34:21.90 SS.reconfigure forSampling @09:34:21.93 SS.seek 25.00ml @09:34:23.62 Vacuum too high -- waiting up to 40s @09:34:24.56 Intake @42.1psia, vacuum from 20.0 to 24.0psi @09:34:24.57 SS.reconfigure forSampling @09:34:24.62 SS.seek 25.00ml @09:34:24.64 delay 3 seconds @09:34:26.65 Vacuum too high -- waiting up to 40s @09:34:27.58 Intake @42.5psia, vacuum from 20.0 to 24.0psi @09:34:27.59 SS.reconfigure forSampling @09:34:27.62 SS.seek 25.00ml @09:34:27.66 ATV.dial :AIV @09:34:28.78 AS.reconfigure ASconfig @09:34:28.81 AS.seek 0.015ml @09:34:29.43 Vacuum too high -- waiting up to 40s @09:34:30.36 Intake @43.0psia, vacuum from 20.0 to 24.0psi @09:34:30.38 SS.reconfigure forSampling @09:34:30.41 SS.seek 25.00ml @09:34:32.09 Vacuum too high -- waiting up to 40s @09:34:32.99 Intake @42.5psia, vacuum from 20.0 to 24.0psi @09:34:33.00 SS.reconfigure forSampling @09:34:33.04 SS.seek 25.00ml @09:34:34.84 Vacuum too high -- waiting up to 40s @09:34:35.73 Intake @42.0psia, vacuum from 20.0 to 24.0psi @09:34:35.75 SS.reconfigure forSampling @09:34:35.78 SS.seek 25.00ml @09:34:37.59 Vacuum too high -- waiting up to 40s @09:34:38.53 Intake @43.4psia, vacuum from 20.0 to 24.0psi @09:34:38.54 SS.reconfigure forSampling @09:34:38.57 SS.seek 25.00ml @09:34:40.15 Vacuum too high -- waiting up to 40s @09:34:40.86 Intake @42.3psia, vacuum from 20.0 to 24.0psi @09:34:40.88 SS.reconfigure forSampling @09:34:40.91 SS.seek 25.00ml @09:34:42.37 Vacuum too high -- waiting up to 40s @09:34:43.31 Intake @42.5psia, vacuum from 20.0 to 24.0psi @09:34:43.32 SS.reconfigure forSampling @09:34:43.36 SS.seek 25.00ml @09:34:45.09 Vacuum too high -- waiting up to 40s @09:34:46.02 Intake @42.3psia, vacuum from 20.0 to 24.0psi @09:34:46.03 SS.reconfigure forSampling @09:34:46.07 SS.seek 25.00ml @09:34:47.81 Vacuum too high -- waiting up to 40s @09:34:48.72 Intake @42.0psia, vacuum from 20.0 to 24.0psi @09:34:48.73 SS.reconfigure forSampling @09:34:48.77 SS.seek 25.00ml @09:34:50.49 Vacuum too high -- waiting up to 40s @09:34:51.41 Intake @42.7psia, vacuum from 20.0 to 24.0psi @09:34:51.42 SS.reconfigure forSampling @09:34:51.46 SS.seek 25.00ml @09:34:53.12 Vacuum too high -- waiting up to 40s @09:34:54.05 Intake @42.7psia, vacuum from 20.0 to 24.0psi @09:34:54.06 SS.reconfigure forSampling @09:34:54.10 SS.seek 25.00ml @09:34:55.71 Vacuum too high -- waiting up to 40s @09:34:56.63 Intake @43.9psia, vacuum from 20.0 to 24.0psi @09:34:56.64 SS.reconfigure forSampling @09:34:56.68 SS.seek 25.00ml @09:34:58.21 Vacuum too high -- waiting up to 40s @09:34:59.13 Intake @43.6psia, vacuum from 20.0 to 24.0psi @09:34:59.14 SS.reconfigure forSampling @09:34:59.18 SS.seek 25.00ml @09:35:00.71 Vacuum too high -- waiting up to 40s @09:35:01.62 Intake @43.2psia, vacuum from 20.0 to 24.0psi @09:35:01.63 SS.reconfigure forSampling @09:35:01.67 SS.seek 25.00ml @09:35:03.18 Vacuum too high -- waiting up to 40s @09:35:04.08 Intake @43.6psia, vacuum from 20.0 to 24.0psi @09:35:04.09 SS.reconfigure forSampling @09:35:04.13 SS.seek 25.00ml @09:35:05.74 Vacuum too high -- waiting up to 40s @09:35:06.65 SS.seek 25.00ml @09:35:08.15 Vacuum too high -- waiting up to 40s @09:35:08.85 Intake @43.2psia, vacuum from 20.0 to 24.0psi @09:35:08.86 SS.reconfigure forSampling @09:35:08.90 SS.seek 25.00ml @09:35:10.15 ATV.dial :waste @09:35:11.48 AS.seek empty @09:35:11.56 Vacuum too high -- waiting up to 40s @09:35:12.49 Intake @44.4psia, vacuum from 20.0 to 24.0psi @09:35:12.51 SS.reconfigure forSampling @09:35:12.54 SS.seek 25.00ml @09:35:12.68 ATV.dial :air3 @09:35:13.26 AS.reconfigure ASslow1 @09:35:13.30 AS.seek 0.009ml @09:35:13.96 Vacuum too high -- waiting up to 40s @09:35:14.67 Intake @43.7psia, vacuum from 20.0 to 24.0psi @09:35:14.69 SS.reconfigure forSampling @09:35:14.72 SS.seek 25.00ml @09:35:15.65 delay 2 seconds @09:35:15.96 Vacuum too high -- waiting up to 40s @09:35:16.67 Intake @43.4psia, vacuum from 20.0 to 24.0psi @09:35:16.68 SS.reconfigure forSampling @09:35:16.72 SS.seek 25.00ml @09:35:17.66 ATV.dial :bleach @09:35:18.06 AS.seek 0.579ml @09:35:18.24 Vacuum too high -- waiting up to 40s @09:35:19.16 Intake @42.8psia, vacuum from 20.0 to 24.0psi @09:35:19.18 SS.reconfigure forSampling @09:35:19.21 SS.seek 25.00ml @09:35:20.93 Vacuum too high -- waiting up to 40s @09:35:21.85 Intake @43.1psia, vacuum from 20.0 to 24.0psi @09:35:21.87 SS.reconfigure forSampling @09:35:21.90 SS.seek 25.00ml @09:35:23.46 Vacuum too high -- waiting up to 40s @09:35:24.39 Intake @43.2psia, vacuum from 20.0 to 24.0psi @09:35:24.40 SS.reconfigure forSampling @09:35:24.44 SS.seek 25.00ml @09:35:26.12 Vacuum too high -- waiting up to 40s @09:35:27.25 Intake @44.3psia, vacuum from 20.0 to 24.0psi @09:35:27.26 SS.reconfigure forSampling @09:35:27.30 SS.seek 25.00ml @09:35:28.84 Vacuum too high -- waiting up to 40s @09:35:29.55 Intake @43.2psia, vacuum from 20.0 to 24.0psi @09:35:29.56 SS.reconfigure forSampling @09:35:29.60 SS.seek 25.00ml @09:35:30.84 Vacuum too high -- waiting up to 40s @09:35:31.54 Intake @42.8psia, vacuum from 20.0 to 24.0psi @09:35:31.56 SS.reconfigure forSampling @09:35:31.59 SS.seek 25.00ml @09:35:33.05 Vacuum too high -- waiting up to 40s @09:35:33.95 Intake @43.0psia, vacuum from 20.0 to 24.0psi @09:35:33.97 SS.reconfigure forSampling @09:35:34.00 SS.seek 25.00ml @09:35:35.58 Vacuum too high -- waiting up to 40s @09:35:36.48 Intake @42.9psia, vacuum from 20.0 to 24.0psi @09:35:36.49 SS.reconfigure forSampling @09:35:36.53 SS.seek 25.00ml @09:35:37.96 Vacuum too high -- waiting up to 40s @09:35:38.86 Intake @43.0psia, vacuum from 20.0 to 24.0psi @09:35:38.87 SS.reconfigure forSampling @09:35:38.91 SS.seek 25.00ml @09:35:40.55 Vacuum too high -- waiting up to 40s @09:35:41.91 Intake @43.7psia, vacuum from 20.0 to 24.0psi @09:35:41.93 SS.reconfigure forSampling @09:35:41.96 SS.seek 25.00ml @09:35:43.68 Vacuum too high -- waiting up to 40s @09:35:44.61 Intake @44.2psia, vacuum from 20.0 to 24.0psi @09:35:44.63 SS.reconfigure forSampling @09:35:44.66 SS.seek 25.00ml @09:35:46.12 Vacuum too high -- waiting up to 40s @09:35:46.83 Intake @43.1psia, vacuum from 20.0 to 24.0psi @09:35:46.85 SS.reconfigure forSampling @09:35:46.88 SS.seek 25.00ml @09:35:48.33 Vacuum too high -- waiting up to 40s @09:35:49.25 Intake @43.2psia, vacuum from 20.0 to 24.0psi @09:35:49.27 SS.reconfigure forSampling @09:35:49.30 SS.seek 25.00ml @09:35:50.80 Vacuum too high -- waiting up to 40s @09:35:51.72 Intake @43.4psia, vacuum from 20.0 to 24.0psi @09:35:51.73 SS.reconfigure forSampling @09:35:51.77 SS.seek 25.00ml @09:35:53.15 Vacuum too high -- waiting up to 40s @09:35:53.85 Intake @43.1psia, vacuum from 20.0 to 24.0psi @09:35:53.87 SS.reconfigure forSampling @09:35:53.90 SS.reconfigure SSconfig @09:35:53.94 SS.hold @09:35:54.02 Intake.select :close @09:35:54.18 ShallowSampler::Clogged in WCR -- progress too slow after 198.2ml @09:35:54.19 SSV.dial :exhaust @09:35:54.98 Exhaust.select :open @09:35:55.08 SS.reconfigure SSexhale @09:35:55.12 SS.seek 0.00ml @09:36:03.37 Exhaust.select :close @09:36:03.48 Sampled 198.2ml @09:36:03.49 collectSample: Complete @09:36:03.50 Drying puck before fixing for 04:00.00 @09:36:03.51 CBV.dial :waste2b @09:36:04.32 delay 1:00 @09:36:06.47 Sleepy.power :sampler,:OFF @09:37:04.32 CSV.dial :air @09:37:04.77 CS.reconfigure CSfast @09:37:04.80 CS.seek 10.000ml @09:37:14.63 delay 3 seconds @09:37:17.64 ATV.dial :air3 @09:37:17.99 delay 3 seconds @09:37:21.05 AS.seek 0.604ml @09:37:26.42 delay 3 seconds @09:37:28.66 CBV.dial between CSV and waste2b @09:37:28.97 Pumping wcr puck from tube 2 up to 55psi... @09:37:29.02 CBV.dial SSV @09:37:29.43 ATV.dial :bleach @09:37:29.82 AS.seek 0.589ml @09:37:30.45 CBV.dial between CSV and waste2b @09:37:31.40 CSV.dial between air and CTV @09:37:31.80 CS.seek 6.559ml @09:37:33.09 delay 3 seconds @09:37:36.10 ATV.dial :ASV @09:37:37.08 ASV.dial :wasteR @09:37:37.75 AS.seek 0.000ml @09:37:40.42 CTV.dial CSV,avoiding => air @09:37:40.44 CSV.dial CTV @09:37:41.27 CS.reconfigure CSpressurize @09:37:41.30 CS.seek 0.000ml @09:37:55.49 CBV.dial :waste2b @09:37:55.81 CS.reconfigure CSconfig @09:37:55.84 CS.seek empty @09:37:59.50 delay 1:00 @09:38:59.51 CTV.dial :air,avoiding => intake @09:39:00.79 Fix #1 of 2 (with rnal) for 02:00.00 @09:39:00.80 CS Pulling 2ml of rnal CSV.dial CSR @09:39:01.31 CSR.select :rnal @09:39:01.41 CS.seek 2.000ml @09:39:07.31 delay 5 seconds @09:39:12.32 CSR.select :air @09:39:12.41 CS.reconfigure CSfast @09:39:12.45 CS.seek 10.000ml @09:39:31.50 CBV.dial between CSV and waste2b @09:39:31.82 Pumping wcr puck from tube 2 up to 55psi... @09:39:31.86 CBV.dial SSV @09:39:33.29 CBV.dial between CSV and waste2b @09:39:34.24 CSV.dial between air and CTV @09:39:35.46 CS.seek 6.661ml @09:39:38.17 delay 1:00 @09:39:43.95 CTV.dial CSV,avoiding => air @09:39:43.97 CSV.dial CTV @09:39:45.27 CS.reconfigure CSpressurize @09:39:45.30 CS.seek 0.000ml @09:40:01.83 delay 2:00 @09:40:38.18 AFV.select :carrierSolution @09:40:38.27 delay 2 seconds @09:40:40.28 AS.reconfigure ASconfig @09:40:40.31 AS.seek 1.000ml @09:41:38.90 delay 1 second @09:41:39.91 AFV.select :holdingCoil @09:41:40.00 delay 1 second @09:41:41.01 ATV.dial :ASV @09:41:41.02 ASV.dial :wasteR @09:41:41.03 AS.reconfigure ASslow1 @09:41:41.06 AS.seek 0.000ml @09:42:01.84 CBV.dial :waste2b @09:42:02.16 CS.reconfigure CSconfig @09:42:02.19 CS.seek empty @09:42:03.65 delay 1:00 @09:43:03.66 CTV.dial :air,avoiding => intake @09:43:04.94 Fix #2 of 2 (with rnal) for 02:00.00 @09:43:04.95 CS Pulling 2ml of rnal CSV.dial CSR @09:43:05.38 CSR.select :rnal @09:43:05.48 CS.seek 2.000ml @09:43:11.40 delay 5 seconds @09:43:16.41 CSR.select :air @09:43:16.50 CS.reconfigure CSfast @09:43:16.54 CS.seek 10.000ml @09:43:35.71 CBV.dial between CSV and waste2b @09:43:36.05 Pumping wcr puck from tube 2 up to 55psi... @09:43:36.10 CBV.dial SSV @09:43:37.54 CBV.dial between CSV and waste2b @09:43:38.48 CSV.dial between air and CTV @09:43:39.14 CS.seek 6.582ml @09:43:47.81 CTV.dial CSV,avoiding => air @09:43:47.83 CSV.dial CTV @09:43:49.20 CS.reconfigure CSpressurize @09:43:49.23 CS.seek 0.000ml @09:44:05.26 delay 2:00 @09:45:05.44 delay 20 seconds @09:45:25.45 ATV.dial :air3 @09:45:26.58 AS.seek 0.590ml @09:46:05.27 CBV.dial :waste2b @09:46:05.59 CS.reconfigure CSconfig @09:46:05.62 CS.seek empty @09:46:07.48 delay 1:00 @09:47:07.50 CTV.dial :air,avoiding => intake @09:47:08.82 Storing wcr puck in Collection Clamp to tube 1 @09:47:08.96 SC.reconfigure SCtight @09:47:08.99 SC.seek 1 @09:47:11.25 SC.reconfigure SCloose @09:47:11.43 Elbow.reconfigure TightElbow @09:47:11.46 Elbow.seek 1 @09:47:13.07 Forearm.reconfigure TightForearm @09:47:13.10 Forearm.seek 1 + 800 counts @09:47:14.16 SE.reconfigure SEpick @09:47:14.21 SE.seek beamBreak @09:47:16.52 SE.configure SEconfig @09:47:16.64 Elbow.reconfigure LimpElbow @09:47:16.68 Forearm.reconfigure LooseForearm @09:47:16.72 Forearm.seek 1 @09:47:16.93 Elbow.hold @09:47:16.99 Elbow.reconfigure TightElbow @09:47:17.07 SE.seek 7.05 Pucks below Top Plate @09:47:17.43 Forearm.coast @09:47:17.46 Forearm.seek 1 + 345 counts @09:47:17.70 Forearm.reconfigure TightForearm @09:47:17.73 Forearm.seek 1 + 800 counts @09:47:17.98 SE.seek 8.05 Pucks below Top Plate @09:47:18.53 Forearm.reconfigure LooseForearm @09:47:18.56 Forearm.seek retract @09:47:19.53 Elbow.seek CC @09:47:21.02 Elbow.reconfigure LooseElbow @09:47:21.10 Forearm.seek CC @09:47:22.06 CC.seek sealed @09:47:22.99 Hand.grip @09:47:24.34 CC.seek open @09:47:27.16 delay 3 seconds @09:47:27.72 Forearm.seek retract @09:47:28.70 Elbow.reconfigure TightElbow @09:47:28.73 Elbow.seek 1 @09:47:30.17 ATV.dial :ASV @09:47:30.36 Elbow.reconfigure LimpElbow @09:47:30.41 Forearm.seek 1 @09:47:31.34 ASV.dial :wasteR @09:47:31.40 AS.seek 0.090ml @09:47:31.45 Elbow.hold @09:47:31.50 Elbow.reconfigure TightElbow @09:47:31.54 Hand.release @09:47:32.35 Forearm.coast @09:47:32.38 Forearm.seek 1 + 329 counts @09:47:32.62 Forearm.reconfigure TightForearm @09:47:32.66 Forearm.seek 1 + 800 counts @09:47:32.94 SE.seek down @09:47:34.04 Forearm.reconfigure LooseForearm @09:47:34.07 Forearm.seek retract @09:47:34.90 SE.coast @09:47:34.99 SC.coast @09:47:35.02 Starting finalPurge @09:47:35.03 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @09:47:35.18 Elbow.seek FlushPuck::Garage @09:47:36.99 Elbow.reconfigure LooseElbow @09:47:37.07 Forearm.seek garage @09:47:38.10 Hand.grip @09:47:38.98 Forearm.seek retract @09:47:40.03 Elbow.reconfigure TightElbow @09:47:40.06 Elbow.seek CC @09:47:42.84 Elbow.reconfigure LooseElbow @09:47:42.92 Forearm.reconfigure TightForearm @09:47:42.95 Forearm.seek CC @09:47:44.12 CC.seek closed @09:47:47.88 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 2.01 @09:47:47.94 Hand.release @09:47:48.68 Forearm.reconfigure LooseForearm @09:47:48.71 Forearm.seek retract @09:47:49.68 Flushing the Collection Valve::Manifold @09:47:49.78 CS Pulling 5ml of flush @09:47:49.79 CSV.dial CSR @09:47:50.69 CSR.select :flush @09:47:50.79 CS.seek 5.000ml @09:48:04.36 delay 5 seconds @09:48:09.37 CSR.select :air @09:48:09.51 CS.reconfigure CSfast @09:48:09.54 CS.seek 7.501ml @09:48:16.17 CSV.dial :waste @09:48:17.45 CS.reconfigure CSconfig @09:48:17.48 CS.seek 0.000ml @09:48:37.33 Flushing lower manifold's waste1 port @09:48:37.42 CSV.dial :flush @09:48:37.85 CS.reconfigure CSfast @09:48:37.88 CS.seek 5.000ml @09:48:50.14 delay 5 seconds @09:48:55.15 CSV.dial :air @09:48:55.64 CS.seek 6.999ml @09:49:01.14 CSV.dial CTV @09:49:01.16
CTV.dial CSV @09:49:02.48 CBV.dial :waste1 @09:49:03.92 CS.seek 0.000ml @09:49:13.61 delay 30 seconds @09:49:20.52
Flushing lower manifold's waste2b port @09:49:20.62 CSV.dial :flush @09:49:21.42 CS.seek 5.000ml @09:49:33.67 delay 5 seconds @09:49:38.68 CSV.dial :air @09:49:39.25 CS.seek 6.999ml @09:49:43.62 ATV.dial :extSrc @09:49:44.14 delay 3 seconds @09:49:44.68 CSV.dial CTV @09:49:44.70
CTV.dial CSV @09:49:45.13 CBV.dial :waste2b @09:49:45.87 CS.seek 0.000ml @09:49:47.15 ATV.dial :ElutionWater @09:49:48.08 AS.seek 0.120ml @09:49:54.50 ATV.dial :air3 @09:49:54.90 AS.seek 0.130ml @09:49:57.21 delay 2 seconds @09:49:59.22 ATV.dial :bleach @09:49:59.69 AS.seek 0.230ml @09:50:02.36
Flushing upper manifold @09:50:02.45 CSV.dial :flush @09:50:03.24 CS.seek 5.000ml @09:50:15.58 delay 5 seconds @09:50:20.33 delay 3 seconds @09:50:20.59
CSV.dial :air @09:50:21.14 CS.seek 7.000ml @09:50:23.35 ATV.dial :air3 @09:50:23.68 delay 3 seconds @09:50:26.57 CSV.dial CBV @09:50:26.59
CBV.dial CSV @09:50:26.73 AS.seek 0.255ml @09:50:27.71
CTV.dial :air @09:50:28.97 CS.seek 0.000ml @09:50:32.11 delay 3 seconds @09:50:35.12 ATV.dial :bleach @09:50:35.51 AS.seek 0.240ml @09:50:38.78 delay 3 seconds @09:50:41.79 ATV.dial :ASV @09:50:42.67 ASV.dial :SPEdelivery @09:50:43.14 AFV.select :carrierSolution @09:50:43.24 delay 2 seconds @09:50:45.25 AS.reconfigure ASconfig @09:50:45.28 AS.seek 0.500ml @09:50:45.48
delay 7 seconds @09:50:52.49 CS.seek 3.000ml @09:51:00.20 CSV.dial :waste @09:51:00.72 CS.seek 0.000ml @09:51:00.76 delay 1 second @09:51:01.77 AFV.select :holdingCoil @09:51:01.91 AS.reconfigure ASslow1 @09:51:01.94 AS.seek 0.170ml @09:51:08.43
CSV.dial :flush @09:51:08.86 CS.seek 3.000ml @09:51:16.57 delay 5 seconds @09:51:21.58 CSV.dial :air @09:51:22.04 CS.seek 5.000ml @09:51:27.54 CSV.dial :air @09:51:27.56 CS.seek 0.000ml @09:51:39.67 delay 7 seconds @09:51:46.68 CS.seek 3.000ml @09:51:54.41 CSV.dial :waste @09:51:55.21 CS.seek 0.000ml @09:52:02.95 Disinfecting external Intake with mfbkill @09:52:03.04 CSV.dial CSR @09:52:04.29 CSR.select :mfbkill @09:52:04.39 CS.seek 8.000ml @09:52:09.53 SPE.hold 2:46:40,95.0C @09:52:09.56 delay 3:00 @09:52:24.07
delay 5 seconds @09:52:29.08 CSR.select :air @09:52:29.17 CS.seek 10.000ml @09:52:34.69 CSV.dial CBV @09:52:34.71
CBV.dial CSV @09:52:35.79 CTV.dial :intake @09:52:36.54 Intake.select :open @09:52:36.63 CS.seek 2.000ml @09:52:56.54 Intake.select :close @09:52:56.63 CTV.dial :air @09:52:57.41 CS.reconfigure CSconfig @09:52:57.44 CS.seek 0.000ml @09:53:03.32 Flushing lower manifold's waste1 port @09:53:03.33 CS Pulling 4ml of mfbkill @09:53:03.34 CSV.dial CSR @09:53:04.41 CSR.select :mfbkill @09:53:04.51 CS.seek 4.000ml @09:53:15.54 delay 5 seconds @09:53:20.55 CSR.select :air @09:53:20.64 CS.reconfigure CSfast @09:53:20.68 CS.seek 10.000ml @09:53:35.76 CSV.dial CTV @09:53:35.78
CTV.dial CSV @09:53:37.13 CBV.dial :waste1 @09:53:37.59 CS.seek 0.000ml @09:54:01.69 Flushing lower manifold's waste2b port @09:54:01.70 CS Pulling 4ml of mfbkill @09:54:01.71 CSV.dial CSR @09:54:02.80 CSR.select :mfbkill @09:54:02.90 CS.reconfigure CSconfig @09:54:02.94 CS.seek 4.000ml @09:54:13.94 delay 5 seconds @09:54:18.95 CSR.select :air @09:54:19.04 CS.reconfigure CSfast @09:54:19.08 CS.seek 10.000ml @09:54:34.07 CSV.dial CTV @09:54:34.09
CTV.dial CSV @09:54:34.62 CBV.dial :waste2b @09:54:35.39 CS.seek 0.000ml @09:54:59.53 Killing upper manifold @09:54:59.54 CS Pulling 4ml of mfbkill @09:54:59.55 CSV.dial CSR @09:55:00.06 CSR.select :mfbkill @09:55:00.16 CS.reconfigure CSconfig @09:55:00.20 CS.seek 4.000ml @09:55:09.58 AFV.select :carrierSolution @09:55:09.67 delay 2 seconds @09:55:11.19
delay 5 seconds @09:55:11.68 AS.reconfigure ASconfig @09:55:11.72 AS.seek 1.000ml @09:55:16.20
CSR.select :air @09:55:16.29 CS.reconfigure CSfast @09:55:16.33 CS.seek 10.000ml @09:55:31.28 CSV.dial CBV @09:55:31.30
CBV.dial CSV @09:55:32.33 CTV.dial :air @09:55:33.66 CS.seek 0.000ml @09:55:57.53 delay 7 seconds @09:56:00.41 delay 1 second @09:56:01.42 AFV.select :holdingCoil @09:56:01.51 delay 1 second @09:56:02.52 ASV.dial :wasteD @09:56:03.81 AS.seek 0.000ml @09:56:04.54
CS.seek 3.000ml @09:56:12.31 CSV.dial :waste @09:56:12.76 CS.seek 0.000ml @09:56:20.46 Flushing upper manifold @09:56:20.55 CSV.dial :flush @09:56:20.99 CS.seek 5.000ml @09:56:33.43 delay 5 seconds @09:56:38.44 CSV.dial :air @09:56:38.92 CS.seek 7.000ml @09:56:44.37 CSV.dial CBV @09:56:44.39
CBV.dial CSV @09:56:45.48 CTV.dial :air @09:56:45.50 CS.seek 0.000ml @09:57:02.37 delay 7 seconds @09:57:02.41 Priming Eluate @09:57:02.42 AFV.select :carrierSolution @09:57:02.51 delay 2 seconds @09:57:04.52 AS.seek 0.500ml @09:57:09.38
CS.seek 3.000ml @09:57:17.12 CSV.dial :waste @09:57:17.63 CS.seek 0.000ml @09:57:25.42 CSV.dial :flush @09:57:25.86 CS.seek 3.000ml @09:57:33.56 delay 5 seconds @09:57:33.94 delay 1 second @09:57:34.95 AFV.select :holdingCoil @09:57:35.04 ATV.dial ASV @09:57:35.05 ASV.dial :SPErecovery @09:57:35.55 Swish #1 of 2 @09:57:35.60 AS.reconfigure ASslow0 @09:57:35.64 AS.seek 0.600ml @09:57:38.57
CSV.dial :air @09:57:39.04 CS.seek 5.001ml @09:57:44.55 CSV.dial :air @09:57:44.57 CS.seek 0.000ml @09:57:46.19 AS.seek 0.500ml @09:57:56.66 Swish #2 of 2 @09:57:56.72 AS.seek 0.600ml @09:57:56.96
delay 7 seconds @09:58:03.97 CS.seek 3.000ml @09:58:07.30 AS.seek 0.500ml @09:58:11.71
CSV.dial :waste @09:58:12.50 CS.seek 0.000ml @09:58:17.80 delay 3 seconds @09:58:20.18
Flushing lower manifold's waste1 port @09:58:20.27 CSV.dial :flush @09:58:20.78 CS.seek 5.000ml @09:58:20.86 AS.reconfigure ASslow1 @09:58:20.89 AS.seek 0.676ml @09:58:33.08
delay 5 seconds @09:58:38.09 CSV.dial :air @09:58:38.56 CS.seek 6.999ml @09:58:44.08 CSV.dial CTV @09:58:44.10
CTV.dial CSV @09:58:45.44 CBV.dial :waste1 @09:58:45.91 CS.seek 0.000ml @09:58:57.13 delay 3 seconds @09:59:00.14 Eluate primed @09:59:00.15 ATV.dial :waste2 @09:59:00.67 AS.reconfigure ASnorm @09:59:00.70 AS.seek 0.000ml @09:59:02.74
Flushing lower manifold's waste2b port @09:59:02.84 CSV.dial :flush @09:59:03.66 CS.seek 5.000ml @09:59:15.93 delay 5 seconds @09:59:20.94 CSV.dial :air @09:59:22.08 CS.seek 6.999ml @09:59:27.49 CSV.dial CTV @09:59:27.51
CTV.dial CSV @09:59:27.95 CBV.dial :waste2b @09:59:28.72 CS.seek 0.000ml @09:59:35.55 SPE.coast @09:59:35.63 ASV.dial :SPErecovery @09:59:35.64 Setting up PCR for HF183em -- writing /var/log/esp/HF183em14mar0609h149ml.pcr @09:59:35.65 Building PCR Slug from ASV with MM2 mix, PP4 primer @09:59:35.66 Priming with carrier @09:59:35.67 ATV.dial ARV @09:59:36.38 ARV.dial :carrier @09:59:36.68 AS.seek 0.500ml @09:59:45.39
Disinfecting external Intake with kill @09:59:45.48 CSV.dial CSR @09:59:45.91 CSR.select :kill @09:59:46.01 CS.seek 8.000ml @10:00:02.56 AFV.select :carrierSolution @10:00:02.67 delay 2 seconds @10:00:04.68 AS.reconfigure ASconfig @10:00:04.72 AS.seek 1.000ml @10:00:05.08
delay 5 seconds @10:00:10.09 CSR.select :air @10:00:10.18 CS.seek 10.000ml @10:00:15.55 CSV.dial CBV @10:00:15.57
CBV.dial CSV @10:00:17.00 CTV.dial :intake @10:00:17.72 Intake.select :open @10:00:17.81 CS.seek 2.000ml @10:00:34.09 delay 1 second @10:00:35.10 AFV.select :holdingCoil @10:00:35.20 delay 1 second @10:00:36.21 ATV.dial :waste @10:00:36.89
Intake.select :close @10:00:36.98 CTV.dial :air @10:00:37.49 AS.reconfigure ASnorm @10:00:37.52 AS.seek empty @10:00:37.70
CS.reconfigure CSconfig @10:00:37.73 CS.seek 0.000ml @10:00:43.64 CSV.dial CSR @10:00:44.69 CSR.select :flush @10:00:44.79 CS.seek 5.000ml @10:00:58.36 delay 5 seconds @10:01:03.37 CSR.select :air @10:01:03.46 CS.seek 10.000ml @10:01:16.92 CSV.dial CBV @10:01:17.98 CS.seek 0.000ml @10:01:29.00 AFV.select :carrierSolution @10:01:29.11 delay 2 seconds @10:01:31.12 AS.reconfigure ASconfig @10:01:31.15 AS.seek 0.500ml @10:01:44.14
Garaging FlushPuck in Collection Clamp @10:01:44.20 Elbow.reconfigure TightElbow @10:01:44.23 Elbow.seek CC @10:01:44.33 Elbow.reconfigure LooseElbow @10:01:44.41 Forearm.seek CC @10:01:45.40 CC.seek sealed @10:01:46.33 Hand.grip @10:01:47.10 CC.seek open @10:01:50.42 Forearm.seek retract @10:01:51.36 Elbow.reconfigure TightElbow @10:01:51.40 Elbow.seek garage @10:01:54.24 Elbow.reconfigure LooseElbow @10:01:54.32 Forearm.reconfigure TightForearm @10:01:54.35 Forearm.seek garage @10:01:55.54 Hand.release @10:01:56.27 Forearm.reconfigure LooseForearm @10:01:56.30 Forearm.seek retract @10:02:00.64 delay 1 second @10:02:01.65 AFV.select :holdingCoil @10:02:01.74 Building Slug @10:02:01.75 ATV.dial :ARV @10:02:03.01 delay 2 seconds @10:02:05.02 ARV.dial :air1 @10:02:05.34 delay 2 seconds @10:02:07.40 AS.reconfigure ASslow2 @10:02:07.43 AS.seek 0.506ml @10:02:13.70 delay 2 seconds @10:02:15.71 ARV.dial :MM2 @10:02:16.95 delay 2 seconds @10:02:18.96 Adding MM2 @10:02:19.01 AS.seek 0.524ml @10:02:37.73 delay 25 seconds @10:03:02.74 ARV.dial :PP4 @10:03:03.17 Adding PP4 @10:03:03.18 delay 2 seconds @10:03:05.24 AS.seek 0.530ml @10:03:11.57 delay 10 seconds @10:03:21.58 ARV.dial :air3 @10:03:22.01 adding ARV air @10:03:22.02 delay 2 seconds @10:03:24.08 AS.seek 0.536ml @10:03:30.43 delay 5 seconds @10:03:35.45 ARV.dial :carrier @10:03:36.50 delay 7 seconds @10:03:43.56 AS.seek 0.594ml @10:04:43.02 delay 5 seconds @10:04:48.03 ATV.dial :ASV @10:04:48.97 delay 2 seconds @10:04:51.03 AS.seek 0.600ml @10:04:57.48 delay 4 seconds @10:05:01.49 ATV.dial ARV @10:05:01.81 delay 2 seconds @10:05:03.82 ARV.dial :carrier @10:05:03.83 delay 2 seconds @10:05:05.88 AS.seek 0.650ml @10:05:57.23 delay 4 seconds @10:06:01.24 ATV.dial PCR @10:06:01.84 ASV.dial :waste1 @10:06:02.51 ARV.dial :air1 @10:06:02.95 Positioning Slug by 0.324ml @10:06:03.01 AS.seek 0.326ml @10:11:33.64 delay 5 seconds @10:11:38.65 ATV.dial ARV @10:11:39.16 ASV.dial :PCRlock @10:11:39.49 delay 5 seconds @10:11:44.50 Running HF183em ProfileEM60C2 thermocycling @10:11:44.51 PCR.program ProfileEM60C2 @10:11:44.62 ProfileEM60C2 should complete in < 2:11:38 @10:11:44.63 PCR.run 2:49:32.5 @12:07:17.65 PCR.off @12:07:18.18 PCR for HF183em completed -- closed /var/log/esp/HF183em14mar0609h149ml.pcr @12:07:18.23 Cleaning PCR @12:07:18.24 AFV.select :carrierSolution @12:07:18.33 delay 2 seconds @12:07:20.34 AS.reconfigure ASconfig @12:07:20.37 AS.seek 0.500ml @12:07:30.81 delay 1 second @12:07:31.82 AFV.select :holdingCoil @12:07:31.91 ASV.dial :waste1 @12:07:32.65 ATV.dial :air3 @12:07:33.59 AS.reconfigure ASslow1 @12:07:33.63 AS.seek 0.510ml @12:07:35.92 delay 2 seconds @12:07:37.93 ATV.dial :bleach @12:07:38.42 AS.seek 0.810ml @12:08:39.79 delay 3 seconds @12:08:42.80 ATV.dial :air3 @12:08:43.12 delay 3 seconds @12:08:46.18 AS.seek 0.835ml @12:08:51.51 delay 3 seconds @12:08:54.52 ATV.dial :bleach @12:08:54.90 AS.seek 0.820ml @12:08:58.21 delay 3 seconds @12:09:01.22 ARV.dial :waste @12:09:01.70 delay 3 seconds @12:09:04.71 ATV.dial :ARV @12:09:05.44 AS.reconfigure ASconfig @12:09:05.48 AS.seek 0.470ml @12:09:26.17 AFV.select :carrierSolution @12:09:26.84 delay 2 seconds @12:09:28.85 AS.seek 1.000ml @12:10:00.04 delay 1 second @12:10:01.05 AFV.select :holdingCoil @12:10:01.14 delay 1 second @12:10:02.15 AS.reconfigure ASfast @12:10:02.18 AS.seek 0.000ml @12:10:29.91 AFV.select :carrierSolution @12:10:30.01 delay 2 seconds @12:10:32.02 AS.reconfigure ASconfig @12:10:32.05 AS.seek 1.000ml @12:11:30.66 delay 1 second @12:11:31.67 AFV.select :holdingCoil @12:11:31.76 delay 1 second @12:11:32.77 AS.reconfigure ASfast @12:11:32.80 AS.seek 0.000ml @12:12:00.49 AFV.select :carrierSolution @12:12:00.59 delay 2 seconds @12:12:02.60 AS.reconfigure ASconfig @12:12:02.63 AS.seek 1.000ml @12:13:01.25 delay 1 second @12:13:02.26 AFV.select :holdingCoil @12:13:02.35 delay 1 second @12:13:03.36 AS.reconfigure ASfast @12:13:03.39 AS.seek 0.000ml @12:13:31.11 ARV.dial between carrier and air1 @12:13:31.52 ATV.dial :air3 @12:13:32.47 AS.reconfigure ASslow1 @12:13:32.50 AS.seek 0.010ml @12:13:34.86 delay 2 seconds @12:13:36.87 ATV.dial :bleach @12:13:37.25 AS.seek 0.810ml @12:16:20.75 delay 3 seconds @12:16:23.76 ATV.dial :air3 @12:16:24.11 delay 3 seconds @12:16:27.17 AS.seek 0.835ml @12:16:32.51 delay 3 seconds @12:16:35.52 ATV.dial :bleach @12:16:36.00 AS.seek 0.820ml @12:16:39.31 delay 3 seconds @12:16:42.32 ATV.dial PCR @12:16:42.77 AS.reconfigure ASconfig @12:16:42.80 AS.seek empty @12:17:30.93 PCR.program :bleachProfile @12:17:30.99 PCR program should complete in < 2:45 @12:17:31.00 PCR.run 10:00 @12:20:32.22 PCR.off @12:20:32.25 AFV.select :carrierSolution @12:20:32.34 delay 2 seconds @12:20:34.35 AS.seek 1.000ml @12:21:33.00 delay 1 second @12:21:34.01 AFV.select :holdingCoil @12:21:34.10 delay 1 second @12:21:35.11 AS.reconfigure ASfast @12:21:35.14 AS.seek 0.000ml @12:22:02.85 delay 3 seconds @12:22:05.86 AFV.select :carrierSolution @12:22:05.95 delay 2 seconds @12:22:07.96 AS.reconfigure ASconfig @12:22:07.99 AS.seek 1.000ml @12:23:06.62 delay 1 second @12:23:07.63 AFV.select :holdingCoil @12:23:07.72 delay 1 second @12:23:08.73 AS.reconfigure ASfast @12:23:08.76 AS.seek 0.000ml @12:23:36.49 delay 3 seconds @12:23:39.50 AFV.select :carrierSolution @12:23:39.59 delay 2 seconds @12:23:41.60 AS.reconfigure ASconfig @12:23:41.63 AS.seek 1.000ml @12:24:40.21 delay 1 second @12:24:41.22 AFV.select :holdingCoil @12:24:41.31 delay 1 second @12:24:42.32 AS.reconfigure ASfast @12:24:42.35 AS.seek 0.000ml @12:25:10.10 delay 3 seconds @12:25:13.11 AFV.select :carrierSolution @12:25:13.20 delay 2 seconds @12:25:15.21 AS.reconfigure ASconfig @12:25:15.24 AS.seek 1.000ml @12:26:13.83 delay 1 second @12:26:14.84 AFV.select :holdingCoil @12:26:14.93 delay 1 second @12:26:15.94 AS.reconfigure ASfast @12:26:15.97 AS.seek 0.000ml @12:26:43.67 delay 3 seconds @12:26:46.68 AFV.select :carrierSolution @12:26:46.77 delay 2 seconds @12:26:48.78 AS.reconfigure ASconfig @12:26:48.81 AS.seek 1.000ml @12:27:47.37 delay 1 second @12:27:48.38 AFV.select :holdingCoil @12:27:48.47 delay 1 second @12:27:49.48 AS.reconfigure ASnorm @12:27:49.51 AS.seek 0.000ml @12:28:41.00 Setting up PCR for ENTap -- writing /var/log/esp/ENTap14mar0612h149ml.pcr @12:28:41.01 Building PCR Slug from ASV with MM1 mix, PP1 primer @12:28:41.02 Priming with carrier @12:28:41.03 ATV.dial ARV @12:28:41.56 ARV.dial :carrier @12:28:41.84 AS.seek 0.500ml @12:29:07.75 AFV.select :carrierSolution @12:29:07.84 delay 2 seconds @12:29:09.85 AS.reconfigure ASconfig @12:29:09.88 AS.seek 1.000ml @12:29:39.31 delay 1 second @12:29:40.32 AFV.select :holdingCoil @12:29:40.41 delay 1 second @12:29:41.42 ATV.dial :waste @12:29:42.69 AS.reconfigure ASnorm @12:29:42.72 AS.seek empty @12:30:34.18 AFV.select :carrierSolution @12:30:34.28 delay 2 seconds @12:30:36.29 AS.reconfigure ASconfig @12:30:36.32 AS.seek 0.500ml @12:31:05.81 delay 1 second @12:31:06.82 AFV.select :holdingCoil @12:31:06.91 Building Slug @12:31:06.92 ATV.dial :ARV @12:31:08.18 delay 2 seconds @12:31:10.19 ARV.dial :air1 @12:31:10.52 delay 2 seconds @12:31:12.58 AS.reconfigure ASslow2 @12:31:12.61 AS.seek 0.506ml @12:31:19.01 delay 2 seconds @12:31:21.02 ARV.dial :MM1 @12:31:21.52 delay 2 seconds @12:31:23.53 Adding MM1 @12:31:23.59 AS.seek 0.524ml @12:31:42.43 delay 25 seconds @12:32:07.98 ARV.dial :PP1 @12:32:08.31 Adding PP1 @12:32:08.32 delay 2 seconds @12:32:10.37 AS.seek 0.530ml @12:32:16.88 delay 10 seconds @12:32:26.89 ARV.dial :air1 @12:32:27.22 adding ARV air @12:32:27.23 delay 2 seconds @12:32:29.28 AS.seek 0.536ml @12:32:35.69 delay 5 seconds @12:32:40.70 ARV.dial :carrier @12:32:41.01 delay 7 seconds @12:32:48.07 AS.seek 0.594ml @12:33:47.61 delay 5 seconds @12:33:52.62 ATV.dial :ASV @12:33:52.94 delay 2 seconds @12:33:55.00 AS.seek 0.600ml @12:34:01.50 delay 4 seconds @12:34:05.51 ATV.dial ARV @12:34:05.94 delay 2 seconds @12:34:07.95 ARV.dial :carrier @12:34:07.96 delay 2 seconds @12:34:10.01 AS.seek 0.650ml @12:35:01.13 delay 4 seconds @12:35:05.14 ATV.dial PCR @12:35:05.66 ASV.dial :waste1 @12:35:05.67 ARV.dial :air1 @12:35:06.01 Positioning Slug by 0.324ml @12:35:06.06 AS.seek 0.326ml @12:40:36.80 delay 5 seconds @12:40:41.81 ATV.dial ARV @12:40:42.33 ASV.dial :PCRlock @12:40:42.75 delay 5 seconds @12:40:47.76 Running ENTap Profile60C thermocycling @12:40:47.77 PCR.program Profile60C @12:40:47.88 Profile60C should complete in < 1:39:08 @12:40:47.89 PCR.run 2:08:55 @14:03:41.68 PCR.off @14:03:42.19 PCR for ENTap completed -- closed /var/log/esp/ENTap14mar0612h149ml.pcr @14:03:42.24 Cleaning PCR @14:03:42.25 AFV.select :carrierSolution @14:03:42.34 delay 2 seconds @14:03:44.35 AS.reconfigure ASconfig @14:03:44.38 AS.seek 0.500ml @14:03:54.82 delay 1 second @14:03:55.83 AFV.select :holdingCoil @14:03:55.92 ASV.dial :waste1 @14:03:56.38 ATV.dial :air3 @14:03:57.31 AS.reconfigure ASslow1 @14:03:57.35 AS.seek 0.510ml @14:03:59.63 delay 2 seconds @14:04:01.64 ATV.dial :bleach @14:04:02.56 AS.seek 0.810ml @14:05:03.99 delay 3 seconds @14:05:07.00 ATV.dial :air3 @14:05:07.41 delay 3 seconds @14:05:10.47 AS.seek 0.835ml @14:05:15.82 delay 3 seconds @14:05:18.83 ATV.dial :bleach @14:05:19.21 AS.seek 0.820ml @14:05:22.54 delay 3 seconds @14:05:25.55 ARV.dial :waste @14:05:26.05 delay 3 seconds @14:05:29.06 ATV.dial :ARV @14:05:29.89 AS.reconfigure ASconfig @14:05:29.93 AS.seek 0.470ml @14:05:50.61 AFV.select :carrierSolution @14:05:50.71 delay 2 seconds @14:05:52.72 AS.seek 1.000ml @14:06:23.89 delay 1 second @14:06:24.90 AFV.select :holdingCoil @14:06:24.99 delay 1 second @14:06:26.00 AS.reconfigure ASfast @14:06:26.03 AS.seek 0.000ml @14:06:53.74 AFV.select :carrierSolution @14:06:53.84 delay 2 seconds @14:06:55.85 AS.reconfigure ASconfig @14:06:55.88 AS.seek 1.000ml @14:07:54.44 delay 1 second @14:07:55.45 AFV.select :holdingCoil @14:07:55.54 delay 1 second @14:07:56.55 AS.reconfigure ASfast @14:07:56.58 AS.seek 0.000ml @14:08:24.30 AFV.select :carrierSolution @14:08:24.40 delay 2 seconds @14:08:26.41 AS.reconfigure ASconfig @14:08:26.44 AS.seek 1.000ml @14:09:25.04 delay 1 second @14:09:26.05 AFV.select :holdingCoil @14:09:26.14 delay 1 second @14:09:27.15 AS.reconfigure ASfast @14:09:27.18 AS.seek 0.000ml @14:09:54.89 ARV.dial between carrier and air1 @14:09:55.29 ATV.dial :air3 @14:09:56.22 AS.reconfigure ASslow1 @14:09:56.25 AS.seek 0.010ml @14:09:58.58 delay 2 seconds @14:10:00.59 ATV.dial :bleach @14:10:00.97 AS.seek 0.810ml @14:12:44.35 delay 3 seconds @14:12:47.36 ATV.dial :air3 @14:12:47.68 delay 3 seconds @14:12:50.74 AS.seek 0.835ml @14:12:56.08 delay 3 seconds @14:12:59.09 ATV.dial :bleach @14:12:59.48 AS.seek 0.820ml @14:13:02.79 delay 3 seconds @14:13:05.80 ATV.dial PCR @14:13:06.13 AS.reconfigure ASconfig @14:13:06.16 AS.seek empty @14:13:54.25 PCR.program :bleachProfile @14:13:54.30 PCR program should complete in < 2:45 @14:13:54.31 PCR.run 10:00 @14:16:52.50 PCR.off @14:16:52.53 AFV.select :carrierSolution @14:16:52.62 delay 2 seconds @14:16:54.63 AS.seek 1.000ml @14:17:53.25 delay 1 second @14:17:54.26 AFV.select :holdingCoil @14:17:54.35 delay 1 second @14:17:55.36 AS.reconfigure ASfast @14:17:55.39 AS.seek 0.000ml @14:18:23.12 delay 3 seconds @14:18:26.13 AFV.select :carrierSolution @14:18:26.22 delay 2 seconds @14:18:28.23 AS.reconfigure ASconfig @14:18:28.26 AS.seek 1.000ml @14:19:26.89 delay 1 second @14:19:27.90 AFV.select :holdingCoil @14:19:27.99 delay 1 second @14:19:29.00 AS.reconfigure ASfast @14:19:29.03 AS.seek 0.000ml @14:19:56.74 delay 3 seconds @14:19:59.75 AFV.select :carrierSolution @14:19:59.84 delay 2 seconds @14:20:01.85 AS.reconfigure ASconfig @14:20:01.88 AS.seek 1.000ml @14:21:00.45 delay 1 second @14:21:01.46 AFV.select :holdingCoil @14:21:01.55 delay 1 second @14:21:02.56 AS.reconfigure ASfast @14:21:02.60 AS.seek 0.000ml @14:21:30.31 delay 3 seconds @14:21:33.32 AFV.select :carrierSolution @14:21:33.41 delay 2 seconds @14:21:35.42 AS.reconfigure ASconfig @14:21:35.45 AS.seek 1.000ml @14:22:34.04 delay 1 second @14:22:35.05 AFV.select :holdingCoil @14:22:35.14 delay 1 second @14:22:36.15 AS.reconfigure ASfast @14:22:36.18 AS.seek 0.000ml @14:23:03.92 delay 3 seconds @14:23:06.93 AFV.select :carrierSolution @14:23:07.02 delay 2 seconds @14:23:09.03 AS.reconfigure ASconfig @14:23:09.06 AS.seek 1.000ml @14:24:07.65 delay 1 second @14:24:08.66 AFV.select :holdingCoil @14:24:08.75 delay 1 second @14:24:09.76 AS.reconfigure ASnorm @14:24:09.79 AS.seek 0.000ml @14:25:01.26 Setting up PCR for ENTem -- writing /var/log/esp/ENTem14mar0614h149ml.pcr @14:25:01.27 Building PCR Slug from ASV with MM2 mix, PP3 primer @14:25:01.28 Priming with carrier @14:25:01.29 ATV.dial ARV @14:25:01.81 ARV.dial :carrier @14:25:02.11 AS.seek 0.500ml @14:25:27.98 AFV.select :carrierSolution @14:25:28.07 delay 2 seconds @14:25:30.08 AS.reconfigure ASconfig @14:25:30.11 AS.seek 1.000ml @14:25:59.55 delay 1 second @14:26:00.56 AFV.select :holdingCoil @14:26:00.65 delay 1 second @14:26:01.66 ATV.dial :waste @14:26:03.02 AS.reconfigure ASnorm @14:26:03.05 AS.seek empty @14:26:54.54 AFV.select :carrierSolution @14:26:54.64 delay 2 seconds @14:26:56.65 AS.reconfigure ASconfig @14:26:57.21 AS.seek 0.500ml @14:27:26.68 delay 1 second @14:27:27.69 AFV.select :holdingCoil @14:27:27.78 Building Slug @14:27:27.79 ATV.dial :ARV @14:27:29.03 delay 2 seconds @14:27:31.04 ARV.dial :air1 @14:27:31.36 delay 2 seconds @14:27:33.42 AS.reconfigure ASslow2 @14:27:33.45 AS.seek 0.506ml @14:27:39.88 delay 2 seconds @14:27:41.89 ARV.dial :MM2 @14:27:43.12 delay 2 seconds @14:27:45.12 Adding MM2 @14:27:45.18 AS.seek 0.524ml @14:28:04.11 delay 25 seconds @14:28:29.12 ARV.dial :PP3 @14:28:29.53 Adding PP3 @14:28:29.54 delay 2 seconds @14:28:31.60 AS.seek 0.530ml @14:28:37.97 delay 10 seconds @14:28:47.98 ARV.dial :air2 @14:28:48.39 adding ARV air @14:28:48.40 delay 2 seconds @14:28:50.46 AS.seek 0.536ml @14:28:56.67 delay 5 seconds @14:29:01.68 ARV.dial :carrier @14:29:02.70 delay 7 seconds @14:29:09.76 AS.seek 0.594ml @14:30:09.26 delay 5 seconds @14:30:14.27 ATV.dial :ASV @14:30:14.59 delay 2 seconds @14:30:16.65 AS.seek 0.600ml @14:30:23.08 delay 4 seconds @14:30:27.09 ATV.dial ARV @14:30:27.42 delay 2 seconds @14:30:29.43 ARV.dial :carrier @14:30:29.44 delay 2 seconds @14:30:31.49 AS.seek 0.650ml @14:31:22.64 delay 4 seconds @14:31:26.65 ATV.dial PCR @14:31:27.15 ASV.dial :waste1 @14:31:27.16 ARV.dial :air1 @14:31:27.51 Positioning Slug by 0.324ml @14:31:27.57 AS.seek 0.326ml @14:36:58.32 delay 5 seconds @14:37:03.33 ATV.dial ARV @14:37:03.92 ASV.dial :PCRlock @14:37:04.42 delay 5 seconds @14:37:09.43 Running ENTem ProfileEM60C thermocycling @14:37:09.44 PCR.program ProfileEM60C @14:37:09.55 ProfileEM60C should complete in < 2:11:38 @14:37:09.56 PCR.run 2:49:32.5 @16:33:06.51 PCR.off @16:33:07.06 PCR for ENTem completed -- closed /var/log/esp/ENTem14mar0614h149ml.pcr @16:33:07.11 Cleaning PCR @16:33:07.12 AFV.select :carrierSolution @16:33:07.21 delay 2 seconds @16:33:09.22 AS.reconfigure ASconfig @16:33:09.25 AS.seek 0.500ml @16:33:19.65 delay 1 second @16:33:20.66 AFV.select :holdingCoil @16:33:20.75 ASV.dial :waste1 @16:33:21.23 ATV.dial :air3 @16:33:22.26 AS.reconfigure ASslow1 @16:33:22.29 AS.seek 0.510ml @16:33:24.57 delay 2 seconds @16:33:26.58 ATV.dial :bleach @16:33:26.97 AS.seek 0.810ml @16:34:28.42 delay 3 seconds @16:34:31.43 ATV.dial :air3 @16:34:31.78 delay 3 seconds @16:34:34.84 AS.seek 0.835ml @16:34:40.15 delay 3 seconds @16:34:43.16 ATV.dial :bleach @16:34:43.65 AS.seek 0.820ml @16:34:46.98 delay 3 seconds @16:34:49.99 ARV.dial :waste @16:34:50.59 delay 3 seconds @16:34:53.60 ATV.dial :ARV @16:34:54.35 AS.reconfigure ASconfig @16:34:54.39 AS.seek 0.470ml @16:35:15.07 AFV.select :carrierSolution @16:35:15.17 delay 2 seconds @16:35:17.18 AS.seek 1.000ml @16:35:48.41 delay 1 second @16:35:49.42 AFV.select :holdingCoil @16:35:49.51 delay 1 second @16:35:50.52 AS.reconfigure ASfast @16:35:50.55 AS.seek 0.000ml @16:36:18.30 AFV.select :carrierSolution @16:36:18.40 delay 2 seconds @16:36:20.41 AS.reconfigure ASconfig @16:36:20.44 AS.seek 1.000ml @16:37:19.01 delay 1 second @16:37:20.02 AFV.select :holdingCoil @16:37:20.11 delay 1 second @16:37:21.12 AS.reconfigure ASfast @16:37:21.15 AS.seek 0.000ml @16:37:48.84 AFV.select :carrierSolution @16:37:48.94 delay 2 seconds @16:37:50.95 AS.reconfigure ASconfig @16:37:50.98 AS.seek 1.000ml @16:38:49.54 delay 1 second @16:38:50.55 AFV.select :holdingCoil @16:38:50.64 delay 1 second @16:38:51.65 AS.reconfigure ASfast @16:38:51.68 AS.seek 0.000ml @16:39:19.39 ARV.dial between carrier and air1 @16:39:19.92 ATV.dial :air3 @16:39:20.87 AS.reconfigure ASslow1 @16:39:20.90 AS.seek 0.010ml @16:39:23.34 delay 2 seconds @16:39:25.35 ATV.dial :bleach @16:39:25.75 AS.seek 0.810ml @16:42:09.14 delay 3 seconds @16:42:12.15 ATV.dial :air3 @16:42:12.49 delay 3 seconds @16:42:15.55 AS.seek 0.835ml @16:42:20.88 delay 3 seconds @16:42:23.89 ATV.dial :bleach @16:42:24.29 AS.seek 0.820ml @16:42:27.61 delay 3 seconds @16:42:30.62 ATV.dial PCR @16:42:30.94 AS.reconfigure ASconfig @16:42:30.97 AS.seek empty @16:43:19.08 PCR.program :bleachProfile @16:43:19.14 PCR program should complete in < 2:45 @16:43:19.15 PCR.run 10:00 @16:46:17.34 PCR.off @16:46:17.37 AFV.select :carrierSolution @16:46:17.46 delay 2 seconds @16:46:19.47 AS.seek 1.000ml @16:47:18.13 delay 1 second @16:47:19.14 AFV.select :holdingCoil @16:47:19.23 delay 1 second @16:47:20.24 AS.reconfigure ASfast @16:47:20.27 AS.seek 0.000ml @16:47:47.98 delay 3 seconds @16:47:50.99 AFV.select :carrierSolution @16:47:51.08 delay 2 seconds @16:47:53.09 AS.reconfigure ASconfig @16:47:53.12 AS.seek 1.000ml @16:48:51.71 delay 1 second @16:48:52.72 AFV.select :holdingCoil @16:48:52.81 delay 1 second @16:48:53.82 AS.reconfigure ASfast @16:48:53.85 AS.seek 0.000ml @16:49:21.55 delay 3 seconds @16:49:24.56 AFV.select :carrierSolution @16:49:24.65 delay 2 seconds @16:49:26.66 AS.reconfigure ASconfig @16:49:26.69 AS.seek 1.000ml @16:50:25.30 delay 1 second @16:50:26.31 AFV.select :holdingCoil @16:50:26.40 delay 1 second @16:50:27.41 AS.reconfigure ASfast @16:50:27.44 AS.seek 0.000ml @16:50:55.14 delay 3 seconds @16:50:58.15 AFV.select :carrierSolution @16:50:58.24 delay 2 seconds @16:51:00.25 AS.reconfigure ASconfig @16:51:00.28 AS.seek 1.000ml @16:51:58.86 delay 1 second @16:51:59.87 AFV.select :holdingCoil @16:51:59.96 delay 1 second @16:52:00.97 AS.reconfigure ASfast @16:52:01.00 AS.seek 0.000ml @16:52:28.73 delay 3 seconds @16:52:31.74 AFV.select :carrierSolution @16:52:31.83 delay 2 seconds @16:52:33.84 AS.reconfigure ASconfig @16:52:33.87 AS.seek 1.000ml @16:53:32.49 delay 1 second @16:53:33.50 AFV.select :holdingCoil @16:53:33.59 delay 1 second @16:53:34.60 AS.reconfigure ASnorm @16:53:34.63 AS.seek 0.000ml @16:54:26.11 Setting up PCR for IPCap -- writing /var/log/esp/IPCap14mar0616h149ml.pcr @16:54:26.65 Building PCR Slug from ASV with MM1 mix, PP2 primer @16:54:26.66 Priming with carrier ATV.dial ARV @16:54:27.18 ARV.dial :carrier @16:54:27.45 AS.seek 0.500ml @16:54:53.36 AFV.select :carrierSolution @16:54:53.47 delay 2 seconds @16:54:55.48 AS.reconfigure ASconfig @16:54:55.52 AS.seek 1.000ml @16:55:24.89 delay 1 second @16:55:25.90 AFV.select :holdingCoil @16:55:25.99 delay 1 second @16:55:27.00 ATV.dial :waste @16:55:28.26 AS.reconfigure ASnorm @16:55:28.29 AS.seek empty @16:56:19.77 AFV.select :carrierSolution @16:56:19.87 delay 2 seconds @16:56:21.88 AS.reconfigure ASconfig @16:56:21.91 AS.seek 0.500ml @16:56:51.38 delay 1 second @16:56:52.39 AFV.select :holdingCoil @16:56:52.48 Building Slug @16:56:52.49 ATV.dial :ARV @16:56:53.74 delay 2 seconds @16:56:55.75 ARV.dial :air1 @16:56:56.08 delay 2 seconds @16:56:58.14 AS.reconfigure ASslow2 @16:56:58.17 AS.seek 0.506ml @16:57:04.41 delay 2 seconds @16:57:06.42 ARV.dial :MM1 @16:57:07.02 delay 2 seconds @16:57:09.03 Adding MM1 @16:57:09.09 AS.seek 0.524ml @16:57:27.91 delay 25 seconds @16:57:52.92 ARV.dial :PP2 @16:57:53.48 Adding PP2 @16:57:53.49 delay 2 seconds @16:57:55.55 AS.seek 0.530ml @16:58:02.15 delay 10 seconds @16:58:12.16 ARV.dial :air2 @16:58:12.57 adding ARV air @16:58:12.58 delay 2 seconds @16:58:14.63 AS.seek 0.536ml @16:58:20.92 delay 5 seconds @16:58:25.93 ARV.dial :carrier @16:58:26.97 delay 7 seconds @16:58:34.03 AS.seek 0.594ml @16:59:33.28 delay 5 seconds @16:59:38.29 ATV.dial :ASV @16:59:38.71 delay 2 seconds @16:59:40.77 AS.seek 0.600ml @16:59:47.12 delay 4 seconds @16:59:51.13 ATV.dial ARV @16:59:51.45 delay 2 seconds @16:59:53.46 ARV.dial :carrier @16:59:53.47 delay 2 seconds @16:59:55.52 AS.seek 0.650ml @17:00:46.84 delay 4 seconds @17:00:50.85 ATV.dial PCR @17:00:51.46 ASV.dial :waste1 @17:00:51.47 ARV.dial :air1 @17:00:51.82 Positioning Slug by 0.324ml @17:00:51.87 AS.seek 0.326ml @17:06:22.56 delay 5 seconds @17:06:27.57 ATV.dial ARV @17:06:28.08 ASV.dial :PCRlock @17:06:28.58 delay 5 seconds @17:06:33.59 Running IPCap Profile59C thermocycling @17:06:33.60 PCR.program Profile59C @17:06:33.70 Profile59C should complete in < 1:39:08 @17:06:33.71 PCR.run 2:08:55 @18:30:05.37 PCR.off @18:30:05.91 PCR for IPCap completed -- closed /var/log/esp/IPCap14mar0616h149ml.pcr @18:30:05.96 Cleaning PCR @18:30:05.97 AFV.select :carrierSolution @18:30:06.06 delay 2 seconds @18:30:08.07 AS.reconfigure ASconfig @18:30:08.11 AS.seek 0.500ml @18:30:18.54 delay 1 second @18:30:19.55 AFV.select :holdingCoil @18:30:19.64 ASV.dial :waste1 @18:30:20.39 ATV.dial :air3 @18:30:21.33 AS.reconfigure ASslow1 @18:30:21.37 AS.seek 0.510ml @18:30:23.68 delay 2 seconds @18:30:25.69 ATV.dial :bleach @18:30:26.06 AS.seek 0.810ml @18:31:27.51 delay 3 seconds @18:31:30.52 ATV.dial :air3 @18:31:30.87 delay 3 seconds @18:31:33.93 AS.seek 0.835ml @18:31:39.27 delay 3 seconds @18:31:42.28 ATV.dial :bleach @18:31:42.67 AS.seek 0.820ml @18:31:45.99 delay 3 seconds @18:31:49.00 ARV.dial :waste @18:31:49.59 delay 3 seconds @18:31:52.60 ATV.dial :ARV @18:31:53.42 AS.reconfigure ASconfig @18:31:53.46 AS.seek 0.470ml @18:32:14.15 AFV.select :carrierSolution @18:32:14.24 delay 2 seconds @18:32:16.25 AS.seek 1.000ml @18:32:47.46 delay 1 second @18:32:48.47 AFV.select :holdingCoil @18:32:48.56 delay 1 second @18:32:49.57 AS.reconfigure ASfast @18:32:49.60 AS.seek 0.000ml @18:33:17.30 AFV.select :carrierSolution @18:33:17.40 delay 2 seconds @18:33:19.41 AS.reconfigure ASconfig @18:33:19.44 AS.seek 1.000ml @18:34:18.03 delay 1 second @18:34:19.04 AFV.select :holdingCoil @18:34:19.13 delay 1 second @18:34:20.14 AS.reconfigure ASfast @18:34:20.17 AS.seek 0.000ml @18:34:47.91 AFV.select :carrierSolution @18:34:48.01 delay 2 seconds @18:34:50.02 AS.reconfigure ASconfig @18:34:50.05 AS.seek 1.000ml @18:35:48.68 delay 1 second @18:35:49.69 AFV.select :holdingCoil @18:35:49.78 delay 1 second @18:35:50.79 AS.reconfigure ASfast @18:35:50.82 AS.seek 0.000ml @18:36:18.56 ARV.dial between carrier and air1 @18:36:18.98 ATV.dial :air3 @18:36:19.93 AS.reconfigure ASslow1 @18:36:19.96 AS.seek 0.010ml @18:36:22.31 delay 2 seconds @18:36:24.32 ATV.dial :bleach @18:36:24.71 AS.seek 0.810ml @18:39:08.13 delay 3 seconds @18:39:11.14 ATV.dial :air3 @18:39:11.49 delay 3 seconds @18:39:14.55 AS.seek 0.835ml @18:39:19.91 delay 3 seconds @18:39:22.92 ATV.dial :bleach @18:39:23.32 AS.seek 0.820ml @18:39:26.58 delay 3 seconds @18:39:29.59 ATV.dial PCR @18:39:29.92 AS.reconfigure ASconfig @18:39:29.95 AS.seek empty @18:40:18.06 PCR.program :bleachProfile @18:40:18.12 PCR program should complete in < 2:45 @18:40:18.13 PCR.run 10:00 @18:43:19.34 PCR.off @18:43:19.37 AFV.select :carrierSolution @18:43:19.46 delay 2 seconds @18:43:21.47 AS.seek 1.000ml @18:44:20.12 delay 1 second @18:44:21.13 AFV.select :holdingCoil @18:44:21.22 delay 1 second @18:44:22.23 AS.reconfigure ASfast @18:44:22.26 AS.seek 0.000ml @18:44:50.01 delay 3 seconds @18:44:53.02 AFV.select :carrierSolution @18:44:53.11 delay 2 seconds @18:44:55.12 AS.reconfigure ASconfig @18:44:55.15 AS.seek 1.000ml @18:45:53.77 delay 1 second @18:45:54.78 AFV.select :holdingCoil @18:45:54.87 delay 1 second @18:45:55.88 AS.reconfigure ASfast @18:45:55.91 AS.seek 0.000ml @18:46:23.66 delay 3 seconds @18:46:26.67 AFV.select :carrierSolution @18:46:26.76 delay 2 seconds @18:46:28.77 AS.reconfigure ASconfig @18:46:28.80 AS.seek 1.000ml @18:47:27.36 delay 1 second @18:47:28.93 AFV.select :holdingCoil @18:47:29.02 delay 1 second @18:47:30.03 AS.reconfigure ASfast @18:47:30.06 AS.seek 0.000ml @18:47:57.80 delay 3 seconds @18:48:00.81 AFV.select :carrierSolution @18:48:00.90 delay 2 seconds @18:48:02.91 AS.reconfigure ASconfig @18:48:02.94 AS.seek 1.000ml @18:49:01.48 delay 1 second @18:49:02.49 AFV.select :holdingCoil @18:49:02.58 delay 1 second @18:49:03.59 AS.reconfigure ASfast @18:49:03.63 AS.seek 0.000ml @18:49:31.34 delay 3 seconds @18:49:34.35 AFV.select :carrierSolution @18:49:34.44 delay 2 seconds @18:49:36.45 AS.reconfigure ASconfig @18:49:36.48 AS.seek 1.000ml @18:50:35.07 delay 1 second @18:50:36.08 AFV.select :holdingCoil @18:50:36.17 delay 1 second @18:50:37.18 AS.reconfigure ASnorm @18:50:37.21 AS.seek 0.000ml @18:51:28.68 Setting up PCR for IPCem -- writing /var/log/esp/IPCem14mar0618h149ml.pcr @18:51:28.69 Building PCR Slug from ASV with MM3 mix, PP5 primer @18:51:28.70 Priming with carrier @18:51:28.71 ATV.dial ARV @18:51:29.23 ARV.dial :carrier @18:51:29.51 AS.seek 0.500ml @18:51:55.38 AFV.select :carrierSolution @18:51:55.49 delay 2 seconds @18:51:57.50 AS.reconfigure ASconfig @18:51:57.54 AS.seek 1.000ml @18:52:26.97 delay 1 second @18:52:27.98 AFV.select :holdingCoil @18:52:28.08 delay 1 second @18:52:29.09 ATV.dial :waste @18:52:30.43 AS.reconfigure ASnorm @18:52:30.46 AS.seek empty @18:53:21.92 AFV.select :carrierSolution @18:53:22.02 delay 2 seconds @18:53:24.03 AS.reconfigure ASconfig @18:53:24.06 AS.seek 0.500ml @18:53:53.55 delay 1 second @18:53:54.56 AFV.select :holdingCoil @18:53:54.65 Building Slug @18:53:54.66 ATV.dial :ARV @18:53:55.91 delay 2 seconds @18:53:57.92 ARV.dial :air1 @18:53:58.25 delay 2 seconds @18:54:00.31 AS.reconfigure ASslow2 @18:54:00.34 AS.seek 0.506ml @18:54:06.83 delay 2 seconds @18:54:08.84 ARV.dial :MM3 @18:54:09.70 delay 2 seconds @18:54:11.71 Adding MM3 @18:54:11.76 AS.seek 0.524ml @18:54:30.48 delay 25 seconds @18:54:55.49 ARV.dial :PP5 @18:54:55.81 Adding PP5 @18:54:55.82 delay 2 seconds @18:54:57.88 AS.seek 0.530ml @18:55:04.31 delay 10 seconds @18:55:14.32 ARV.dial :air3 @18:55:14.64 adding ARV air @18:55:14.65 delay 2 seconds @18:55:16.71 AS.seek 0.536ml @18:55:23.20 delay 5 seconds @18:55:28.21 ARV.dial :carrier @18:55:29.25 delay 7 seconds @18:55:36.31 AS.seek 0.594ml @18:56:35.78 delay 5 seconds @18:56:40.79 ATV.dial :ASV @18:56:41.12 delay 2 seconds @18:56:43.18 AS.seek 0.600ml @18:56:49.71 delay 4 seconds @18:56:53.72 ATV.dial ARV @18:56:54.15 delay 2 seconds @18:56:56.16 ARV.dial :carrier @18:56:56.17 delay 2 seconds @18:56:58.22 AS.seek 0.650ml @18:57:49.90 delay 4 seconds @18:57:53.91 ATV.dial PCR @18:57:54.43 ASV.dial :waste1 @18:57:54.44 ARV.dial :air1 @18:57:54.86 Positioning Slug by 0.324ml @18:57:54.91 AS.seek 0.326ml @19:03:25.75 delay 5 seconds @19:03:30.76 ATV.dial ARV @19:03:31.28 ASV.dial :PCRlock @19:03:31.73 delay 5 seconds @19:03:36.74 Running IPCem ProfileEM59C thermocycling @19:03:36.75 PCR.program ProfileEM59C @19:03:36.86 ProfileEM59C should complete in < 2:11:38 @19:03:36.87 PCR.run 2:49:32.5 @20:59:13.29 PCR.off @20:59:13.83 PCR for IPCem completed -- closed /var/log/esp/IPCem14mar0618h149ml.pcr @20:59:13.84 Full MFB Decontamination @20:59:13.85 Decon extraction column and decon AIV @20:59:13.86 SPE.hold 2:46:40,55.0C @20:59:13.88 ASV.dial :SPEdelivery @20:59:14.52 delay 20 seconds @20:59:34.53 AFV.select :carrierSolution @20:59:34.62 delay 2 seconds @20:59:36.63 AS.reconfigure ASconfig @20:59:36.66 AS.seek 0.500ml @20:59:47.03 delay 1 second @20:59:48.04 AFV.select :holdingCoil @20:59:48.13 ATV.dial :air1 @20:59:49.65 AS.reconfigure ASslow1 @20:59:49.68 AS.seek 0.520ml @20:59:54.25 ATV.dial :ASV @20:59:55.53 ASV.dial :SPErecovery @20:59:56.08 AS.seek 0.620ml @21:00:16.72 ATV.dial :waste @21:00:18.26 AS.reconfigure ASnorm @21:00:18.29 AS.seek empty @21:00:50.34 AFV.select :holdingCoil @21:00:50.35 ATV.dial :waste @21:00:50.36 AS.reconfigure ASconfig @21:00:50.39 AS.seek 0.000ml @21:00:50.67 AFV.select :carrierSolution @21:00:50.76 delay 2 seconds @21:00:52.77 AS.seek 0.500ml @21:01:22.17 delay 1 second @21:01:23.18 AFV.select :holdingCoil @21:01:23.27 ATV.dial :air3 @21:01:23.82 AS.reconfigure ASslow1 @21:01:23.86 AS.seek 0.520ml @21:01:28.20 ATV.dial :air3 @21:01:28.26 AS.seek 0.530ml @21:01:30.55 delay 2 seconds @21:01:32.56 ATV.dial :bleach @21:01:32.94 AS.seek 0.710ml @21:02:09.90 delay 3 seconds @21:02:12.91 ATV.dial :air3 @21:02:13.25 delay 3 seconds @21:02:16.31 AS.seek 0.735ml @21:02:21.65 delay 3 seconds @21:02:24.66 ATV.dial :bleach @21:02:25.05 AS.seek 0.720ml @21:02:28.36 delay 3 seconds @21:02:31.37 ATV.dial ASV @21:02:32.36 ASV.dial :SPErecovery @21:02:32.42 AS.seek 0.365ml @21:03:45.04 ATV.dial :waste @21:03:46.52 AS.reconfigure ASnorm @21:03:46.55 AS.seek empty @21:04:05.57 ATV.dial :air3 @21:04:06.13 AS.reconfigure ASslow1 @21:04:06.17 AS.seek 0.009ml @21:04:08.49 delay 2 seconds @21:04:10.50 ATV.dial :bleach @21:04:10.97 AS.seek 0.579ml @21:06:07.38 delay 3 seconds @21:06:10.39 ATV.dial :air3 @21:06:10.71 delay 3 seconds @21:06:13.77 AS.seek 0.604ml @21:06:19.05 delay 3 seconds @21:06:22.06 ATV.dial :bleach @21:06:22.45 AS.seek 0.589ml @21:06:25.74 delay 3 seconds @21:06:28.75 ATV.dial :ASV @21:06:29.63 ASV.dial :wasteR @21:06:30.39 AS.seek 0.000ml @21:08:30.94 delay 1:00 @21:09:30.96 ATV.dial ASV @21:09:30.97 AFV.select :carrierSolution @21:09:31.06 delay 2 seconds @21:09:33.07 AS.reconfigure ASconfig @21:09:33.10 AS.seek 1.000ml @21:10:31.63 delay 1 second @21:10:32.64 AFV.select :holdingCoil @21:10:32.73 delay 1 second @21:10:33.74 ASV.dial :wasteR @21:10:33.75 AS.reconfigure ASslow1 @21:10:33.78 AS.seek 0.000ml @21:13:58.17 delay 10 seconds @21:14:08.18 ATV.dial :air3 @21:14:09.89 AS.seek 0.010ml @21:14:12.19 delay 2 seconds @21:14:14.20 ATV.dial :bleach @21:14:14.60 AS.seek 0.580ml @21:16:11.09 delay 3 seconds @21:16:14.10 ATV.dial :air3 @21:16:14.45 delay 3 seconds @21:16:17.51 AS.seek 0.605ml @21:16:22.89 delay 3 seconds @21:16:25.90 ATV.dial :bleach @21:16:26.30 AS.seek 0.590ml @21:16:29.71 delay 3 seconds @21:16:32.72 ATV.dial ASV @21:16:33.59 ASV.dial :SPEdelivery @21:16:34.35 AS.seek 0.000ml @21:18:34.95 delay 1:00 @21:19:34.96 AFV.select :carrierSolution @21:19:35.06 delay 2 seconds @21:19:37.07 AS.reconfigure ASconfig @21:19:37.10 AS.seek 1.000ml @21:20:35.69 delay 1 second @21:20:36.70 AFV.select :holdingCoil @21:20:36.80 delay 1 second @21:20:37.81 ATV.dial ASV @21:20:37.82 ASV.dial :SPEdelivery @21:20:37.83 AS.reconfigure ASslow1 @21:20:37.86 AS.seek 0.000ml @21:24:02.11 delay 10 seconds @21:24:12.12 ATV.dial :air2 @21:24:12.94 AS.reconfigure ASslow0 @21:24:12.97 AS.seek 0.800ml @21:25:34.88 delay 3 seconds @21:25:37.89 ATV.dial :ASV @21:25:38.65 ASV.dial :SPEdelivery @21:25:38.67 AS.reconfigure ASslow1 @21:25:38.70 AS.seek 0.030ml @21:28:16.01 delay 20 seconds @21:28:36.02 ATV.dial :waste,via => air3 @21:28:37.46 AS.reconfigure ASconfig @21:28:37.49 AS.seek empty @21:28:39.59 SPE.coast @21:28:39.67 Removing Bleach from Mixing Coil @21:28:39.68 AFV.select :carrierSolution @21:28:39.77 delay 2 seconds @21:28:41.78 AS.seek 0.500ml @21:29:11.26 delay 1 second @21:29:12.27 AFV.select :holdingCoil @21:29:12.36 ATV.dial :ASV @21:29:13.81 ASV.dial :SPErecovery @21:29:14.63 AS.reconfigure ASslow1 @21:29:14.66 AS.seek 0.730ml @21:30:01.89 ASV.dial :wasteD @21:30:02.55 AS.reconfigure ASnorm @21:30:02.58 AS.seek 0.000ml @21:30:40.21 AFV.select :carrierSolution @21:30:40.31 delay 2 seconds @21:30:42.32 AS.reconfigure ASconfig @21:30:42.35 AS.seek 0.500ml @21:31:11.77 delay 1 second @21:31:12.78 AFV.select :holdingCoil @21:31:12.87 ATV.dial ASV @21:31:12.88 ASV.dial :SPErecovery @21:31:13.59 AS.reconfigure ASslow0 @21:31:13.62 AS.seek 0.300ml @21:31:34.32 ATV.dial :air2 @21:31:35.17 AS.reconfigure ASslow1 @21:31:35.21 AS.seek 0.320ml @21:31:39.55 ATV.dial ASV @21:31:40.40 AS.reconfigure ASslow0 @21:31:40.43 AS.seek 0.570ml @21:32:06.23 ASV.dial :wasteD @21:32:06.89 AS.reconfigure ASnorm @21:32:06.92 AS.seek 0.000ml @21:32:36.35 AFV.select :carrierSolution @21:32:36.45 delay 2 seconds @21:32:38.46 AS.reconfigure ASconfig @21:32:38.49 AS.seek 0.500ml @21:33:07.91 delay 1 second @21:33:08.92 AFV.select :holdingCoil @21:33:09.01 ATV.dial ASV @21:33:09.02 ASV.dial :SPErecovery @21:33:09.72 AS.reconfigure ASslow0 @21:33:09.76 AS.seek 0.300ml @21:33:30.46 ATV.dial :air2 @21:33:31.29 AS.reconfigure ASslow1 @21:33:31.33 AS.seek 0.320ml @21:33:35.68 ATV.dial ASV @21:33:36.43 AS.reconfigure ASslow0 @21:33:36.46 AS.seek 0.570ml @21:34:02.22 ASV.dial :wasteD @21:34:02.89 AS.reconfigure ASnorm @21:34:02.92 AS.seek 0.000ml @21:34:32.38 ATV.dial :air2 @21:34:33.20 AS.reconfigure ASslow0 @21:34:33.23 AS.seek 0.300ml @21:35:04.19 ATV.dial :ASV @21:35:04.99 ASV.dial :wasteD @21:35:05.01 AS.reconfigure ASconfig @21:35:05.04 AS.seek 0.050ml @21:35:19.89 ATV.dial :waste @21:35:21.34 AS.seek 0.000ml @21:35:24.53 ASV.dial :SPEdelivery @21:35:25.55 deconLysateINT @21:35:25.56 ATV.dial :air3 @21:35:26.12 AS.reconfigure ASslow1 @21:35:26.16 AS.seek 0.010ml @21:35:28.50 delay 2 seconds @21:35:30.50 ATV.dial :bleach @21:35:30.88 AS.seek 0.960ml @21:38:44.85 delay 3 seconds @21:38:47.86 ATV.dial :air3 @21:38:48.20 delay 3 seconds @21:38:51.26 AS.seek 0.985ml @21:38:56.59 delay 3 seconds @21:38:59.60 ATV.dial :bleach @21:39:00.09 AS.seek 0.970ml @21:39:03.40 delay 3 seconds @21:39:06.41 ATV.dial :AIV @21:39:08.15 AIV.dial :coilToMFB @21:39:08.16 AWV.select :waste @21:39:08.17 AFV.select :carrierSolution @21:39:08.26 delay 2 seconds @21:39:10.27 AS.reconfigure ASconfig @21:39:10.30 AS.seek 1.000ml @21:39:12.38 delay 1 second @21:39:13.39 AFV.select :holdingCoil @21:39:13.48 delay 1 second @21:39:14.49 AS.reconfigure ASslow0 @21:39:14.52 AS.seek empty @21:40:57.05 AFV.select :carrierSolution @21:40:57.15 delay 2 seconds @21:40:59.16 AS.reconfigure ASconfig @21:40:59.20 AS.seek 1.000ml @21:41:57.82 delay 1 second @21:41:58.83 AFV.select :holdingCoil @21:41:58.92 delay 1 second @21:41:59.93 AS.reconfigure ASslow0 @21:41:59.96 AS.seek empty @21:43:42.35 AFV.select :carrierSolution @21:43:42.45 delay 2 seconds @21:43:44.46 AS.reconfigure ASconfig @21:43:44.50 AS.seek 1.000ml @21:44:43.15 delay 1 second @21:44:44.16 AFV.select :holdingCoil @21:44:44.25 delay 1 second @21:44:45.26 AS.reconfigure ASslow0 @21:44:45.29 AS.seek empty @21:46:27.69 ATV.dial :air2 @21:46:28.36 AS.reconfigure ASslow1 @21:46:28.40 AS.seek 0.009ml @21:46:30.74 AS.reconfigure ASslow0 @21:46:30.78 AS.seek 1.000ml @21:48:12.11 ATV.dial AIV @21:48:12.64 AS.seek 0.020ml @21:49:52.95 ATV.dial :waste @21:49:54.23 AS.seek 0.000ml @21:49:56.55 AWV.select :air @21:49:56.70 Cleaning PCR @21:49:56.71 AFV.select :carrierSolution @21:49:56.80 delay 2 seconds @21:49:58.81 AS.reconfigure ASconfig @21:49:58.84 AS.seek 0.500ml @21:50:28.28 delay 1 second @21:50:29.29 AFV.select :holdingCoil @21:50:29.38 ASV.dial :waste1 @21:50:29.89 ATV.dial :air3 @21:50:30.45 AS.reconfigure ASslow1 @21:50:30.48 AS.seek 0.510ml @21:50:32.76 delay 2 seconds @21:50:34.77 ATV.dial :bleach @21:50:35.14 AS.seek 0.810ml @21:51:36.60 delay 3 seconds @21:51:39.61 ATV.dial :air3 @21:51:39.95 delay 3 seconds @21:51:43.01 AS.seek 0.835ml @21:51:48.35 delay 3 seconds @21:51:51.36 ATV.dial :bleach @21:51:51.75 AS.seek 0.820ml @21:51:55.07 delay 3 seconds @21:51:58.08 ARV.dial :waste @21:51:58.57 delay 3 seconds @21:52:01.58 ATV.dial :ARV @21:52:02.32 AS.reconfigure ASconfig @21:52:02.36 AS.seek 0.470ml @21:52:23.04 AFV.select :carrierSolution @21:52:23.13 delay 2 seconds @21:52:25.14 AS.seek 1.000ml @21:52:56.37 delay 1 second @21:52:57.38 AFV.select :holdingCoil @21:52:57.47 delay 1 second @21:52:58.48 AS.reconfigure ASfast @21:52:58.51 AS.seek 0.000ml @21:53:26.19 AFV.select :carrierSolution @21:53:26.30 delay 2 seconds @21:53:28.31 AS.reconfigure ASconfig @21:53:28.34 AS.seek 1.000ml @21:54:26.93 delay 1 second @21:54:27.94 AFV.select :holdingCoil @21:54:28.03 delay 1 second @21:54:29.04 AS.reconfigure ASfast @21:54:29.07 AS.seek 0.000ml @21:54:56.80 AFV.select :carrierSolution @21:54:56.90 delay 2 seconds @21:54:58.91 AS.reconfigure ASconfig @21:54:58.94 AS.seek 1.000ml @21:55:57.53 delay 1 second @21:55:58.54 AFV.select :holdingCoil @21:55:58.75 delay 1 second @21:55:59.76 AS.reconfigure ASfast @21:55:59.79 AS.seek 0.000ml @21:56:27.55 ARV.dial between carrier and air1 @21:56:28.06 ATV.dial :air3 @21:56:29.01 AS.reconfigure ASslow1 @21:56:29.04 AS.seek 0.010ml @21:56:31.39 delay 2 seconds @21:56:33.40 ATV.dial :bleach @21:56:33.88 AS.seek 0.810ml @21:59:17.28 delay 3 seconds @21:59:20.29 ATV.dial :air3 @21:59:20.62 delay 3 seconds @21:59:23.68 AS.seek 0.835ml @21:59:29.03 delay 3 seconds @21:59:32.04 ATV.dial :bleach @21:59:32.44 AS.seek 0.820ml @21:59:35.78 delay 3 seconds @21:59:38.79 ATV.dial PCR @21:59:39.12 AS.reconfigure ASconfig @21:59:39.15 AS.seek empty @22:00:27.29 PCR.program :bleachProfile @22:00:27.34 PCR program should complete in < 2:45 @22:00:27.35 PCR.run 10:00 @22:03:31.58 PCR.off @22:03:31.61 AFV.select :carrierSolution @22:03:31.70 delay 2 seconds @22:03:33.71 AS.seek 1.000ml @22:04:32.37 delay 1 second @22:04:33.38 AFV.select :holdingCoil @22:04:33.47 delay 1 second @22:04:34.48 AS.reconfigure ASfast @22:04:35.05 AS.seek 0.000ml @22:05:02.79 delay 3 seconds @22:05:05.80 AFV.select :carrierSolution @22:05:05.89 delay 2 seconds @22:05:07.90 AS.reconfigure ASconfig @22:05:07.93 AS.seek 1.000ml @22:06:06.52 delay 1 second @22:06:07.53 AFV.select :holdingCoil @22:06:07.62 delay 1 second @22:06:08.63 AS.reconfigure ASfast @22:06:08.69 AS.seek 0.000ml @22:06:36.41 delay 3 seconds @22:06:39.42 AFV.select :carrierSolution @22:06:39.51 delay 2 seconds @22:06:41.52 AS.reconfigure ASconfig @22:06:41.55 AS.seek 1.000ml @22:07:40.15 delay 1 second @22:07:41.16 AFV.select :holdingCoil @22:07:41.25 delay 1 second @22:07:42.26 AS.reconfigure ASfast @22:07:42.29 AS.seek 0.000ml @22:08:10.02 delay 3 seconds @22:08:13.03 AFV.select :carrierSolution @22:08:13.12 delay 2 seconds @22:08:15.13 AS.reconfigure ASconfig @22:08:15.16 AS.seek 1.000ml @22:09:13.72 delay 1 second @22:09:14.73 AFV.select :holdingCoil @22:09:14.82 delay 1 second @22:09:15.83 AS.reconfigure ASfast @22:09:15.86 AS.seek 0.000ml @22:09:43.61 delay 3 seconds @22:09:46.62 AFV.select :carrierSolution @22:09:46.71 delay 2 seconds @22:09:48.72 AS.reconfigure ASconfig @22:09:48.75 AS.seek 1.000ml @22:10:47.30 delay 1 second @22:10:48.31 AFV.select :holdingCoil @22:10:48.40 delay 1 second @22:10:49.41 AS.reconfigure ASnorm @22:10:49.44 AS.seek 0.000ml @22:11:40.83 AFV.select :carrierSolution @22:11:40.92 delay 2 seconds @22:11:42.93 AS.reconfigure ASconfig @22:11:42.96 AS.seek 1.000ml @22:12:41.57 delay 1 second @22:12:42.58 AFV.select :holdingCoil @22:12:42.67 delay 1 second @22:12:43.68 ATV.dial :waste @22:12:44.58 AS.reconfigure ASnorm @22:12:44.61 AS.seek 0.500ml @22:13:10.54 ATV.dial :waste2 @22:13:11.63 AS.seek 0.000ml @22:13:37.53
Cleaning PCR AFV.select :carrierSolution @22:13:37.62 delay 2 seconds @22:13:39.63 AS.reconfigure ASconfig @22:13:39.66 AS.seek 0.500ml @22:14:09.09 delay 1 second @22:14:10.10 AFV.select :holdingCoil @22:14:10.19 ASV.dial :waste1 @22:14:10.20 ATV.dial :air3 @22:14:11.68 AS.reconfigure ASslow1 @22:14:11.71 AS.seek 0.510ml @22:14:13.99 delay 2 seconds @22:14:16.00 ATV.dial :bleach @22:14:16.48 AS.seek 0.810ml @22:15:17.90 delay 3 seconds @22:15:20.92 ATV.dial :air3 @22:15:21.24 delay 3 seconds @22:15:24.30 AS.seek 0.835ml @22:15:29.63 delay 3 seconds @22:15:32.65 ATV.dial :bleach @22:15:33.13 AS.seek 0.820ml @22:15:36.51 delay 3 seconds @22:15:39.53 ARV.dial :waste @22:15:39.93 delay 3 seconds @22:15:42.94 ATV.dial :ARV @22:15:43.69 AS.reconfigure ASconfig @22:15:43.73 AS.seek 0.470ml @22:16:04.41 AFV.select :carrierSolution @22:16:04.50 delay 2 seconds @22:16:06.51 AS.seek 1.000ml @22:16:37.72 delay 1 second @22:16:38.73 AFV.select :holdingCoil @22:16:38.82 delay 1 second @22:16:39.83 AS.reconfigure ASfast @22:16:39.86 AS.seek 0.000ml @22:17:07.56 AFV.select :carrierSolution @22:17:07.65 delay 2 seconds @22:17:09.66 AS.reconfigure ASconfig @22:17:09.69 AS.seek 1.000ml @22:18:08.26 delay 1 second @22:18:09.27 AFV.select :holdingCoil @22:18:09.36 delay 1 second @22:18:10.37 AS.reconfigure ASfast @22:18:10.41 AS.seek 0.000ml @22:18:38.12 AFV.select :carrierSolution @22:18:38.21 delay 2 seconds @22:18:40.22 AS.reconfigure ASconfig @22:18:40.25 AS.seek 1.000ml @22:19:38.88 delay 1 second @22:19:39.89 AFV.select :holdingCoil @22:19:39.98 delay 1 second @22:19:40.99 AS.reconfigure ASfast @22:19:41.02 AS.seek 0.000ml @22:20:08.73 ARV.dial between carrier and air1 @22:20:09.15 ATV.dial :air3 @22:20:10.08 AS.reconfigure ASslow1 @22:20:10.12 AS.seek 0.010ml @22:20:12.45 delay 2 seconds @22:20:14.46 ATV.dial :bleach @22:20:14.85 AS.seek 0.810ml @22:22:58.24 delay 3 seconds @22:23:01.25 ATV.dial :air3 @22:23:01.60 delay 3 seconds @22:23:04.66 AS.seek 0.835ml @22:23:10.03 delay 3 seconds @22:23:13.04 ATV.dial :bleach @22:23:13.43 AS.seek 0.820ml @22:23:16.70 delay 3 seconds @22:23:19.71 ATV.dial PCR @22:23:20.03 AS.reconfigure ASconfig @22:23:20.06 AS.seek empty @22:24:08.17 PCR.program :bleachProfile @22:24:08.23 PCR program should complete in < 2:45 @22:24:08.24 PCR.run 10:00 @22:27:12.97 PCR.off @22:27:13.00 AFV.select :carrierSolution @22:27:13.09 delay 2 seconds @22:27:15.10 AS.seek 1.000ml @22:28:13.76 delay 1 second @22:28:14.77 AFV.select :holdingCoil @22:28:14.86 delay 1 second @22:28:15.87 AS.reconfigure ASfast @22:28:15.90 AS.seek 0.000ml @22:28:43.63 delay 3 seconds @22:28:46.64 AFV.select :carrierSolution @22:28:46.73 delay 2 seconds @22:28:48.74 AS.reconfigure ASconfig @22:28:48.78 AS.seek 1.000ml @22:29:47.36 delay 1 second @22:29:48.37 AFV.select :holdingCoil @22:29:48.46 delay 1 second @22:29:49.47 AS.reconfigure ASfast @22:29:49.50 AS.seek 0.000ml @22:30:17.22 delay 3 seconds @22:30:20.23 AFV.select :carrierSolution @22:30:20.32 delay 2 seconds @22:30:22.33 AS.reconfigure ASconfig @22:30:22.36 AS.seek 1.000ml @22:31:20.99 delay 1 second @22:31:22.00 AFV.select :holdingCoil @22:31:22.09 delay 1 second @22:31:23.10 AS.reconfigure ASfast @22:31:23.13 AS.seek 0.000ml @22:31:50.84 delay 3 seconds @22:31:53.85 AFV.select :carrierSolution @22:31:53.94 delay 2 seconds @22:31:55.95 AS.reconfigure ASconfig @22:31:55.98 AS.seek 1.000ml @22:32:54.56 delay 1 second @22:32:55.58 AFV.select :holdingCoil @22:32:55.67 delay 1 second @22:32:56.68 AS.reconfigure ASfast @22:32:56.72 AS.seek 0.000ml @22:33:24.45 delay 3 seconds @22:33:27.46 AFV.select :carrierSolution @22:33:27.55 delay 2 seconds @22:33:29.56 AS.reconfigure ASconfig @22:33:29.59 AS.seek 1.000ml @22:34:28.21 delay 1 second @22:34:29.22 AFV.select :holdingCoil @22:34:29.31 delay 1 second @22:34:30.32 AS.reconfigure ASnorm @22:34:30.35 AS.seek 0.000ml @22:35:21.79 Completed {wqPCR} begun 08:00:31 @22:35:21.82 Asleep til 08:00PST07-Mar-14 @22:35:21.83 AS.stop @22:35:21.84
Sleeping until 08:00PST07-Mar-14 @22:35:21.87 GoodNews.email "Then, in tube 2: wqPCR\n/home/esp/esp2local/mission/14mar05sccwrpDeploy.rb:64",Subject => "Asleep until 08:00PST07-Mar-14" @22:35:23.66 CC.stop @22:35:23.68 CS.stop @22:35:23.71 PC.stop @22:35:23.74 PS.stop @22:35:23.77 Forearm.stop @22:35:23.80 Elbow.stop @22:35:23.83 SS.stop @22:35:23.90 Sleepy.power :raw,:OFF @22:35:23.91 Sleepy.power :dwarf,:OFF Shutdown @08:00:31.85PST07-Mar-14
Powering up Dwarves @08:00:37.05 Sleepy.power :raw,:ON @08:00:37.51 SPE.configure SPEconfig @08:00:37.53 AS.configure ASconfig @08:00:37.57 CC.configure CCconfig @08:00:37.60 CS.configure CSconfig @08:00:37.63 PC.configure PCconfig @08:00:37.66 PS.configure PSconfig @08:00:37.69 Forearm.configure LooseForearm @08:00:37.72 Elbow.configure LooseElbow @08:00:37.75 SC.configure SCloose @08:00:37.78 SE.configure SEconfig @08:00:37.81 SS.configure SSconfig @08:00:37.84 CH.configure CHconfig @08:00:37.86 PH.configure PHconfig @08:00:37.89 Valve.reset @08:00:38.55 PCR.off @08:00:38.58 PCR.pidtemp= 50,0.1,0,300,25,25 @08:00:38.61 PCR.caltemp= 6.6661,0.0344,0.0 @08:00:38.71 Intake.select :close @08:00:38.74 Exhaust.select :close @08:00:38.75 CTV.dial :air @08:00:38.79 PSV.dial :air @08:00:38.82
PC.reconfigure PCconfig @08:00:38.87 PC.home.seek open @08:00:38.92 CSV.dial :waste1 @08:00:39.27 PS.reconfigure PSconfig @08:00:39.31 PS.home.seek 0.000ml @08:00:39.86 SSV.dial :spit,avoiding => exhaust @08:00:40.65 CS.reconfigure CSfast @08:00:40.68 SS.reconfigure SSconfig @08:00:40.77 SS.home.seek 0.00ml @08:00:40.79 CS.home.seek 0.000ml @08:00:40.86 CC.reconfigure CCconfig @08:00:40.90 CC.home.seek open @08:00:49.62 PS.coast @08:00:50.06
CC.seek open @08:00:50.08 PC.seek open @08:00:50.23
Forearm.reconfigure LooseForearm @08:00:50.27 Forearm.home.seek retract @08:00:51.02 Hand.grip @08:00:51.05
Elbow.jog -1500 @08:00:51.64 Elbow.reconfigure LooseElbow @08:00:51.67 Elbow.home.seek clear @08:00:56.42 SE.reconfigure SEconfig @08:00:56.45 SE.home.seek down @08:00:57.04 SC.reconfigure SCsloppy @08:00:57.08 SC.home.seek initial @08:00:59.26 Forearm.seek retract @08:00:59.31 Elbow.seek hammer @08:01:00.47 Forearm.reconfigure TightForearm @08:01:00.52 Forearm.seek 2 @08:01:01.66 Forearm.seek retract @08:01:02.75 Forearm.reconfigure LooseForearm @08:01:03.16PST07-Mar-14 ===> STARTED wqPCR (phase #4) <=== @08:01:03.17 Starting initialPurge @08:01:03.18 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:01:03.23 Hand.release @08:01:04.06 Elbow.reconfigure TightElbow @08:01:04.10 Elbow.seek FlushPuck::Garage @08:01:05.85 Elbow.reconfigure LooseElbow @08:01:05.93 Forearm.seek garage @08:01:06.90 Hand.grip @08:01:07.70 Forearm.seek retract @08:01:08.72 Elbow.reconfigure TightElbow @08:01:08.75 Elbow.seek CC @08:01:11.50 Elbow.reconfigure LooseElbow @08:01:11.59 Forearm.reconfigure TightForearm @08:01:11.62 Forearm.seek CC @08:01:12.73 CC.seek closed @08:01:16.46 Collection Clamp onSpring/offSpring for 5/22 samples, on/off torque 1.90 @08:01:16.51 Hand.release @08:01:17.27 Forearm.reconfigure LooseForearm @08:01:17.30 Forearm.seek retract @08:01:18.25 Flushing the Collection Valve::Manifold @08:01:18.34 CS Pulling 5ml of flush @08:01:18.35 CSV.dial CSR @08:01:19.62 CSR.select :flush @08:01:19.72 CS.reconfigure CSconfig @08:01:19.76 CS.seek 5.000ml @08:01:33.24 delay 5 seconds @08:01:38.25 CSR.select :air @08:01:38.39 CS.reconfigure CSfast @08:01:38.42 CS.seek 7.499ml @08:01:45.12 CSV.dial :waste @08:01:46.37 CS.reconfigure CSconfig @08:01:46.41 CS.seek 0.000ml @08:02:06.24 Purging external Intake @08:02:06.33 CSV.dial :flush @08:02:06.77 CS.reconfigure CSfast @08:02:06.80 CS.seek 10.000ml @08:02:30.62 delay 5 seconds @08:02:35.63 CSV.dial CBV @08:02:35.65
CBV.dial CSV @08:02:36.44 CTV.dial :intake @08:02:37.18 Intake.select :open @08:02:37.27 CS.seek 0.000ml @08:03:01.61 Intake.select :close @08:03:01.70 CTV.dial :air @08:03:02.43 Flushing upper manifold @08:03:02.52 CSV.dial :flush @08:03:03.33 CS.seek 5.000ml @08:03:15.74 delay 5 seconds @08:03:20.75 CSV.dial :air @08:03:21.63 CS.seek 7.000ml @08:03:27.08 CSV.dial CBV @08:03:27.10
CBV.dial CSV @08:03:28.16 CTV.dial :air @08:03:28.18 CS.seek 0.000ml @08:03:45.02 delay 7 seconds @08:03:52.03 CS.seek 3.000ml @08:03:59.83 CSV.dial :waste @08:04:00.34 CS.seek 0.000ml @08:04:08.35 CSV.dial :flush @08:04:08.78 CS.seek 3.000ml @08:04:16.52 delay 5 seconds @08:04:21.53 CSV.dial :air @08:04:22.00 CS.seek 5.000ml @08:04:27.52 CSV.dial :air @08:04:27.54 CS.seek 0.000ml @08:04:39.77 delay 7 seconds @08:04:46.78 CS.seek 3.000ml @08:04:54.55 CSV.dial :waste @08:04:55.34 CS.seek 0.000ml @08:05:03.05 Garaging FlushPuck in Collection Clamp @08:05:03.10 Elbow.reconfigure TightElbow @08:05:03.14 Elbow.seek CC @08:05:03.23 Elbow.reconfigure LooseElbow @08:05:03.31 Forearm.seek CC @08:05:04.27 CC.seek sealed @08:05:05.20 Hand.grip @08:05:05.97 CC.seek open @08:05:09.26 Forearm.seek retract @08:05:10.24 Elbow.reconfigure TightElbow @08:05:10.28 Elbow.seek garage @08:05:13.61 Elbow.reconfigure LooseElbow @08:05:13.69 Forearm.reconfigure TightForearm @08:05:13.72 Forearm.seek garage @08:05:14.88 Hand.release @08:05:15.64 Forearm.reconfigure LooseForearm @08:05:15.67 Forearm.seek retract @08:05:16.79 Starting mfb @08:05:16.80 pcrBAC sampling at most 1000ml, wcr at most 1000ml @08:05:16.82 Loading sh1 puck from tube 2 into Collection Clamp @08:05:16.85 BACfans.email "Can@08:01:03, 16.1C, 62% humidity, 14.2psia, 13.724V, 0.616A, 0.616A avg, 8.45W\nCTD data unavailable\nISUS data unavailable",Subject => "pcrBAC sampling at most 1000ml, wcr at most 1000ml" @08:05:18.16 Readying the MFB @08:05:18.17 Homing MFB Syringe @08:05:18.18 AFV.select :holdingCoil @08:05:18.43 ATV.dial :air2 @08:05:18.48
SC.reconfigure SCloose @08:05:18.66 Elbow.reconfigure TightElbow @08:05:18.71 Elbow.seek 2 @08:05:19.73 AS.reconfigure ASconfig @08:05:19.76 AS.home.seek home @08:05:20.63
Forearm.reconfigure TightForearm @08:05:20.66 Forearm.seek 2 + 800 counts @08:05:21.73 SE.reconfigure SEpick @08:05:21.78 SE.seek beamBreak @08:05:23.52 SE.configure SEconfig @08:05:23.65 Elbow.seek 2 @08:05:23.89 Forearm.seek 2 + 300 counts @08:05:24.15 Forearm.reconfigure LooseForearm @08:05:24.19 Hand.grip @08:05:24.98 SE.seek down @08:05:25.12
Forearm.seek retract @08:05:26.07 Elbow.seek CC @08:05:26.56 SE.coast @08:05:27.25 AFV.select :carrierSolution @08:05:27.35 delay 2 seconds @08:05:27.61
Elbow.reconfigure LooseElbow @08:05:27.69 Forearm.reconfigure TightForearm @08:05:27.72 Forearm.seek CC @08:05:28.84 CC.seek closed @08:05:29.38 AS.seek 1.000ml @08:05:32.64
Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.95 @08:05:32.65 Hand.release @08:05:33.39 Forearm.reconfigure LooseForearm @08:05:33.42 Forearm.seek retract @08:05:34.42 SC.coast @08:05:34.45 collectSample 1000ml w/bubble point of 55psi Priming 7ml @08:05:34.46 Sleepy.power :sampler,:ON @08:05:34.47 Intake.select :close Exhaust.select :close @08:05:34.48 SS.hold @08:05:34.64 Calibrating pressure and temperature sensors @08:05:34.66 CTV.dial :air @08:05:34.67 SSV.dial :spit,avoiding => exhaust @08:05:34.68 SS.reconfigure SSfast @08:05:34.71 SS.seek 5.00ml @08:05:37.65 SSV.dial CBV @08:05:37.67
CBV.dial SSV @08:05:39.04 delay 3.8 seconds @08:05:42.92 SSV.dial :spit @08:05:44.27 SS.seek 0.00ml @08:05:46.84 SSV.dial CBV @08:05:48.18 CTV.dial :intake @08:05:48.95 Waiting for external sampler to finish priming... @08:05:48.96 delayUntil "08:15:34.46" @08:06:21.09 delay 1 second @08:06:22.10 AFV.select :holdingCoil @08:06:22.19 delay 1 second @08:06:23.20 ATV.dial :waste @08:06:24.58 AS.reconfigure ASnorm @08:06:24.61 AS.home.seek empty @08:07:17.76 ATV.dial :waste @08:07:17.77 ARV.dial between carrier and air1 @08:07:17.84 ASV.dial :waste1 @08:15:34.47 SSV.dial CBV @08:15:34.49
CBV.dial SSV @08:15:34.50 Allowing 55s for 7.0ml of remaining 7.0ml Intake.select :open @08:15:36.63 Intake @50.7psia, vacuum from 20.0 to 24.0psi @08:15:36.64 SS.reconfigure forSampling @08:15:36.68 SS.seek 7.00ml @08:15:42.44 Stroke completed -- waiting for pressure to equalize ... @08:15:43.08 Intake.select :close @08:15:43.21 SSV.dial :exhaust @08:15:43.97 Exhaust.select :open @08:15:44.08 SS.reconfigure SSexhale @08:15:44.12 SS.seek 0.00ml @08:15:48.08 Exhaust.select :close @08:15:48.18 Sampling 1000.0ml @08:15:48.19 Intake.select :close @08:15:48.20 Exhaust.select :close @08:15:48.21 SS.reconfigure SSconfig @08:15:48.24 SS.hold @08:15:48.40 CTV.dial :intake @08:15:48.41 SSV.dial CBV @08:15:48.43
CBV.dial SSV @08:15:49.22 Allowing 185s for 25.0ml of remaining 1000.0ml Intake.select :open @08:15:51.35 Intake @50.7psia, vacuum from 20.0 to 24.0psi @08:15:51.36 SS.reconfigure forSampling @08:15:51.40 SS.seek 25.00ml @08:16:06.72 Stroke completed -- waiting for pressure to equalize ... @08:16:07.36 Intake.select :close @08:16:07.49 SSV.dial :exhaust @08:16:08.25 Exhaust.select :open @08:16:08.34 SS.reconfigure SSexhale @08:16:08.38 SS.seek 0.00ml @08:16:17.44 Exhaust.select :close @08:16:17.53 SSV.dial CBV @08:16:17.55
CBV.dial SSV @08:16:18.32 Allowing 185s for 25.0ml of remaining 975.0ml @08:16:18.33 Intake.select :open @08:16:20.46 Intake @50.3psia, vacuum from 20.0 to 24.0psi @08:16:20.47 SS.reconfigure forSampling @08:16:20.51 SS.seek 25.00ml @08:16:24.22 Vacuum too high -- waiting up to 40s @08:16:24.49 Intake @43.0psia, vacuum from 20.0 to 24.0psi @08:16:24.50 SS.reconfigure forSampling @08:16:24.53 SS.seek 25.00ml @08:16:31.78 Vacuum too high -- waiting up to 40s @08:16:32.05 Intake @43.5psia, vacuum from 20.0 to 24.0psi @08:16:32.06 SS.reconfigure forSampling @08:16:32.09 SS.seek 25.00ml @08:16:34.38 Vacuum too high -- waiting up to 40s @08:16:34.86 Intake @48.2psia, vacuum from 20.0 to 24.0psi @08:16:34.87 SS.reconfigure forSampling @08:16:34.90 SS.seek 25.00ml @08:16:37.94 Vacuum too high -- waiting up to 40s @08:16:38.20 Intake @46.3psia, vacuum from 20.0 to 24.0psi @08:16:38.21 SS.reconfigure forSampling @08:16:38.25 SS.seek 25.00ml @08:16:39.86 Stroke completed -- waiting for pressure to equalize ... @08:16:40.50 Intake.select :close @08:16:40.63 SSV.dial :exhaust @08:16:41.41 Exhaust.select :open @08:16:41.50 SS.reconfigure SSexhale @08:16:41.54 SS.seek 0.00ml @08:16:50.64 Exhaust.select :close @08:16:50.73 SSV.dial CBV @08:16:50.75
CBV.dial SSV @08:16:51.53 Allowing 185s for 25.0ml of remaining 950.0ml @08:16:51.54 Intake.select :open @08:16:53.67 Intake @51.0psia, vacuum from 20.0 to 24.0psi @08:16:53.68 SS.reconfigure forSampling @08:16:53.72 SS.seek 25.00ml @08:16:56.54 Vacuum too high -- waiting up to 40s @08:16:56.80 Intake @47.2psia, vacuum from 20.0 to 24.0psi @08:16:56.81 SS.reconfigure forSampling @08:16:56.84 SS.seek 25.00ml @08:17:01.31 Vacuum too high -- waiting up to 40s @08:17:01.57 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:17:01.58 SS.reconfigure forSampling @08:17:01.62 SS.seek 25.00ml @08:17:07.44 Vacuum too high -- waiting up to 40s @08:17:07.69 Intake @48.4psia, vacuum from 20.0 to 24.0psi @08:17:07.70 SS.reconfigure forSampling @08:17:07.74 SS.seek 25.00ml @08:17:18.50 Vacuum too high -- waiting up to 40s @08:17:18.77 Intake @48.8psia, vacuum from 20.0 to 24.0psi @08:17:18.78 SS.reconfigure forSampling @08:17:18.81 SS.seek 25.00ml @08:17:38.86 Stroke completed -- waiting for pressure to equalize ... @08:17:39.50 Intake.select :close @08:17:39.63 SSV.dial :exhaust @08:17:40.41 Exhaust.select :open @08:17:40.51 SS.reconfigure SSexhale @08:17:40.55 SS.seek 0.00ml @08:17:49.66 Exhaust.select :close @08:17:49.75 SSV.dial CBV @08:17:49.77
CBV.dial SSV @08:17:50.55 Allowing 185s for 25.0ml of remaining 925.0ml @08:17:50.56 Intake.select :open @08:17:52.69 Intake @50.0psia, vacuum from 20.0 to 24.0psi @08:17:52.70 SS.reconfigure forSampling @08:17:52.74 SS.seek 25.00ml @08:18:06.46 Vacuum too high -- waiting up to 40s @08:18:06.72 Intake @49.3psia, vacuum from 20.0 to 24.0psi @08:18:06.73 SS.reconfigure forSampling @08:18:07.29 SS.seek 25.00ml @08:19:32.77 Stroke completed -- waiting for pressure to equalize ... @08:19:34.15 Intake.select :close @08:19:34.29 SSV.dial :exhaust @08:19:35.07 Exhaust.select :open @08:19:35.17 SS.reconfigure SSexhale @08:19:35.21 SS.seek 0.00ml @08:19:44.26 Exhaust.select :close @08:19:44.35 SSV.dial CBV @08:19:44.37
CBV.dial SSV @08:19:45.17 Allowing 185s for 25.0ml of remaining 900.0ml @08:19:45.18 Intake.select :open @08:19:47.31 Intake @48.5psia, vacuum from 20.0 to 24.0psi @08:19:47.32 SS.reconfigure forSampling @08:19:47.36 SS.seek 25.00ml @08:20:57.70 Vacuum too high -- waiting up to 40s @08:20:57.97 Intake @47.8psia, vacuum from 20.0 to 24.0psi @08:20:57.98 SS.reconfigure forSampling @08:20:58.01 SS.seek 25.00ml @08:21:07.59 Vacuum too high -- waiting up to 40s @08:21:07.85 Intake @48.0psia, vacuum from 20.0 to 24.0psi @08:21:07.86 SS.reconfigure forSampling @08:21:07.89 SS.seek 25.00ml @08:21:11.02 Vacuum too high -- waiting up to 40s @08:21:11.29 Intake @48.2psia, vacuum from 20.0 to 24.0psi @08:21:11.30 SS.reconfigure forSampling @08:21:11.33 SS.seek 25.00ml @08:21:14.80 Vacuum too high -- waiting up to 40s @08:21:15.07 Intake @48.2psia, vacuum from 20.0 to 24.0psi @08:21:15.08 SS.reconfigure forSampling @08:21:15.11 SS.seek 25.00ml @08:21:17.09 Vacuum too high -- waiting up to 40s @08:21:17.35 Intake @48.2psia, vacuum from 20.0 to 24.0psi @08:21:17.36 SS.reconfigure forSampling @08:21:17.39 SS.seek 25.00ml @08:21:19.15 Vacuum too high -- waiting up to 40s @08:21:19.41 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:21:19.42 SS.reconfigure forSampling @08:21:19.45 SS.seek 25.00ml @08:21:21.37 Vacuum too high -- waiting up to 40s @08:21:21.63 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:21:21.64 SS.reconfigure forSampling @08:21:21.68 SS.seek 25.00ml @08:21:25.33 Vacuum too high -- waiting up to 40s @08:21:25.59 Intake @48.2psia, vacuum from 20.0 to 24.0psi @08:21:25.60 SS.reconfigure forSampling @08:21:25.64 SS.seek 25.00ml @08:21:27.06 Vacuum too high -- waiting up to 40s @08:21:27.32 Intake @48.1psia, vacuum from 20.0 to 24.0psi @08:21:27.33 SS.reconfigure forSampling @08:21:27.36 SS.seek 25.00ml @08:21:29.15 Vacuum too high -- waiting up to 40s @08:21:29.41 Intake @48.4psia, vacuum from 20.0 to 24.0psi @08:21:29.42 SS.reconfigure forSampling @08:21:29.45 SS.seek 25.00ml @08:21:31.09 Vacuum too high -- waiting up to 40s @08:21:31.35 Intake @47.9psia, vacuum from 20.0 to 24.0psi @08:21:31.36 SS.reconfigure forSampling @08:21:31.39 SS.seek 25.00ml @08:21:33.09 Vacuum too high -- waiting up to 40s @08:21:33.35 Intake @48.2psia, vacuum from 20.0 to 24.0psi @08:21:33.36 SS.reconfigure forSampling @08:21:33.39 SS.seek 25.00ml @08:21:34.90 Vacuum too high -- waiting up to 40s @08:21:35.16 Intake @48.0psia, vacuum from 20.0 to 24.0psi @08:21:35.17 SS.reconfigure forSampling @08:21:35.20 SS.seek 25.00ml @08:21:36.81 Vacuum too high -- waiting up to 40s @08:21:37.07 Intake @48.0psia, vacuum from 20.0 to 24.0psi @08:21:37.08 SS.reconfigure forSampling @08:21:37.11 SS.seek 25.00ml @08:21:39.09 Vacuum too high -- waiting up to 40s @08:21:39.35 Intake @49.1psia, vacuum from 20.0 to 24.0psi @08:21:39.36 SS.reconfigure forSampling @08:21:39.39 SS.seek 25.00ml @08:21:40.52 Vacuum too high -- waiting up to 40s @08:21:40.78 Intake @48.3psia, vacuum from 20.0 to 24.0psi @08:21:40.79 SS.reconfigure forSampling @08:21:40.82 SS.seek 25.00ml @08:21:42.24 Vacuum too high -- waiting up to 40s @08:21:42.50 Intake @48.2psia, vacuum from 20.0 to 24.0psi @08:21:42.51 SS.reconfigure forSampling @08:21:42.55 SS.seek 25.00ml @08:21:44.24 Vacuum too high -- waiting up to 40s @08:21:44.50 Intake @47.9psia, vacuum from 20.0 to 24.0psi @08:21:44.51 SS.reconfigure forSampling @08:21:44.54 SS.seek 25.00ml @08:21:45.49 Stroke completed -- waiting for pressure to equalize ... @08:21:47.29 Intake.select :close @08:21:47.44 SSV.dial :exhaust @08:21:48.22 Exhaust.select :open @08:21:48.31 SS.reconfigure SSexhale @08:21:48.35 SS.seek 0.00ml @08:21:57.19 Exhaust.select :close @08:21:57.28 SSV.dial CBV @08:21:57.30
CBV.dial SSV @08:21:58.08 Allowing 185s for 25.0ml of remaining 875.0ml @08:21:58.09 Intake.select :open @08:22:00.77 Intake @48.2psia, vacuum from 20.0 to 24.0psi @08:22:00.78 SS.reconfigure forSampling @08:22:00.82 SS.seek 25.00ml @08:22:03.65 Vacuum too high -- waiting up to 40s @08:22:03.91 Intake @48.5psia, vacuum from 20.0 to 24.0psi @08:22:03.92 SS.reconfigure forSampling @08:22:03.96 SS.seek 25.00ml @08:22:05.52 Vacuum too high -- waiting up to 40s @08:22:05.78 Intake @47.9psia, vacuum from 20.0 to 24.0psi @08:22:05.80 SS.reconfigure forSampling @08:22:05.83 SS.seek 25.00ml @08:22:07.46 Vacuum too high -- waiting up to 40s @08:22:07.72 Intake @48.1psia, vacuum from 20.0 to 24.0psi @08:22:07.73 SS.reconfigure forSampling @08:22:07.76 SS.seek 25.00ml @08:22:09.02 Vacuum too high -- waiting up to 40s @08:22:09.28 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:22:09.33 SS.reconfigure forSampling @08:22:09.37 SS.seek 25.00ml @08:22:10.87 Vacuum too high -- waiting up to 40s @08:22:11.13 Intake @48.0psia, vacuum from 20.0 to 24.0psi @08:22:11.14 SS.reconfigure forSampling @08:22:11.17 SS.seek 25.00ml @08:22:12.68 Vacuum too high -- waiting up to 40s @08:22:12.94 Intake @48.3psia, vacuum from 20.0 to 24.0psi @08:22:12.96 SS.reconfigure forSampling @08:22:12.99 SS.seek 25.00ml @08:22:14.15 Vacuum too high -- waiting up to 40s @08:22:14.41 Intake @48.6psia, vacuum from 20.0 to 24.0psi @08:22:14.42 SS.reconfigure forSampling @08:22:14.45 SS.seek 25.00ml @08:22:15.96 Vacuum too high -- waiting up to 40s @08:22:16.22 Intake @48.8psia, vacuum from 20.0 to 24.0psi @08:22:16.23 SS.reconfigure forSampling @08:22:16.26 SS.seek 25.00ml @08:22:17.40 Vacuum too high -- waiting up to 40s @08:22:17.66 Intake @48.0psia, vacuum from 20.0 to 24.0psi @08:22:17.67 SS.reconfigure forSampling @08:22:17.71 SS.seek 25.00ml @08:22:19.08 Vacuum too high -- waiting up to 40s @08:22:19.34 Intake @47.9psia, vacuum from 20.0 to 24.0psi @08:22:19.35 SS.reconfigure forSampling @08:22:19.39 SS.seek 25.00ml @08:22:20.65 Vacuum too high -- waiting up to 40s @08:22:20.91 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:22:20.92 SS.reconfigure forSampling @08:22:20.95 SS.seek 25.00ml @08:22:22.18 Vacuum too high -- waiting up to 40s @08:22:22.44 Intake @47.4psia, vacuum from 20.0 to 24.0psi @08:22:22.45 SS.reconfigure forSampling @08:22:22.48 SS.seek 25.00ml @08:22:23.71 Vacuum too high -- waiting up to 40s @08:22:23.97 Intake @47.3psia, vacuum from 20.0 to 24.0psi @08:22:23.98 SS.reconfigure forSampling @08:22:24.02 SS.seek 25.00ml @08:22:25.12 Vacuum too high -- waiting up to 40s @08:22:25.38 Intake @47.7psia, vacuum from 20.0 to 24.0psi @08:22:25.39 SS.reconfigure forSampling @08:22:25.42 SS.seek 25.00ml @08:22:26.62 Vacuum too high -- waiting up to 40s @08:22:26.88 Intake @48.1psia, vacuum from 20.0 to 24.0psi @08:22:26.89 SS.reconfigure forSampling @08:22:26.92 SS.seek 25.00ml @08:22:27.96 Vacuum too high -- waiting up to 40s @08:22:28.22 SS.seek 25.00ml @08:22:29.33 Vacuum too high -- waiting up to 40s @08:22:29.59 Intake @49.0psia, vacuum from 20.0 to 24.0psi @08:22:29.60 SS.reconfigure forSampling @08:22:29.63 SS.seek 25.00ml @08:22:30.55 Vacuum too high -- waiting up to 40s @08:22:30.81 Intake @48.5psia, vacuum from 20.0 to 24.0psi @08:22:30.82 SS.reconfigure forSampling @08:22:30.85 SS.seek 25.00ml @08:22:31.90 Vacuum too high -- waiting up to 40s @08:22:32.16 Intake @48.3psia, vacuum from 20.0 to 24.0psi @08:22:32.17 SS.reconfigure forSampling @08:22:32.20 SS.seek 25.00ml @08:22:33.24 Vacuum too high -- waiting up to 40s @08:22:33.50 Intake @47.9psia, vacuum from 20.0 to 24.0psi @08:22:33.51 SS.reconfigure forSampling @08:22:33.54 SS.seek 25.00ml @08:22:34.58 Vacuum too high -- waiting up to 40s @08:22:34.84 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:22:34.85 SS.reconfigure forSampling @08:22:34.88 SS.seek 25.00ml @08:22:35.90 Vacuum too high -- waiting up to 40s @08:22:36.16 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:22:36.17 SS.reconfigure forSampling @08:22:36.20 SS.seek 25.00ml @08:22:37.33 Vacuum too high -- waiting up to 40s @08:22:37.59 Intake @47.5psia, vacuum from 20.0 to 24.0psi @08:22:37.60 SS.reconfigure forSampling @08:22:37.63 SS.seek 25.00ml @08:22:38.61 Vacuum too high -- waiting up to 40s @08:22:38.87 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:22:38.88 SS.reconfigure forSampling @08:22:39.45 SS.seek 25.00ml @08:22:41.05 Vacuum too high -- waiting up to 40s @08:22:41.31 Intake @47.5psia, vacuum from 20.0 to 24.0psi @08:22:41.32 SS.reconfigure forSampling @08:22:41.35 SS.seek 25.00ml @08:22:42.46 Vacuum too high -- waiting up to 40s @08:22:42.72 Intake @48.2psia, vacuum from 20.0 to 24.0psi @08:22:42.73 SS.reconfigure forSampling @08:22:42.76 SS.seek 25.00ml @08:22:43.96 Vacuum too high -- waiting up to 40s @08:22:44.22 Intake @49.3psia, vacuum from 20.0 to 24.0psi @08:22:44.23 SS.reconfigure forSampling @08:22:44.27 SS.seek 25.00ml @08:22:45.05 Vacuum too high -- waiting up to 40s @08:22:45.31 Intake @48.2psia, vacuum from 20.0 to 24.0psi @08:22:45.32 SS.reconfigure forSampling @08:22:45.36 SS.seek 25.00ml @08:22:46.33 Vacuum too high -- waiting up to 40s @08:22:46.59 Intake @47.9psia, vacuum from 20.0 to 24.0psi @08:22:46.61 SS.reconfigure forSampling @08:22:46.64 SS.seek 25.00ml @08:22:47.71 Vacuum too high -- waiting up to 40s @08:22:47.97 Intake @47.9psia, vacuum from 20.0 to 24.0psi @08:22:47.98 SS.reconfigure forSampling @08:22:48.02 SS.seek 25.00ml @08:22:49.11 Vacuum too high -- waiting up to 40s @08:22:49.37 Intake @48.0psia, vacuum from 20.0 to 24.0psi @08:22:49.38 SS.reconfigure forSampling @08:22:49.42 SS.seek 25.00ml @08:22:50.40 Vacuum too high -- waiting up to 40s @08:22:50.66 Intake @48.0psia, vacuum from 20.0 to 24.0psi @08:22:50.67 SS.reconfigure forSampling @08:22:50.70 SS.seek 25.00ml @08:22:51.68 Vacuum too high -- waiting up to 40s @08:22:51.94 Intake @47.9psia, vacuum from 20.0 to 24.0psi @08:22:51.95 SS.reconfigure forSampling @08:22:51.99 SS.seek 25.00ml @08:22:52.99 Vacuum too high -- waiting up to 40s @08:22:53.25 Intake @47.7psia, vacuum from 20.0 to 24.0psi @08:22:53.26 SS.reconfigure forSampling @08:22:53.29 SS.seek 25.00ml @08:22:54.27 Vacuum too high -- waiting up to 40s @08:22:54.53 Intake @47.4psia, vacuum from 20.0 to 24.0psi @08:22:54.54 SS.reconfigure forSampling @08:22:54.57 SS.seek 25.00ml @08:22:55.49 Vacuum too high -- waiting up to 40s @08:22:55.75 Intake @47.2psia, vacuum from 20.0 to 24.0psi @08:22:55.76 SS.reconfigure forSampling @08:22:55.79 SS.seek 25.00ml @08:22:56.86 Vacuum too high -- waiting up to 40s @08:22:57.12 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:22:57.13 SS.reconfigure forSampling @08:22:57.16 SS.seek 25.00ml @08:22:58.18 Vacuum too high -- waiting up to 40s @08:22:58.44 Intake @48.9psia, vacuum from 20.0 to 24.0psi @08:22:58.45 SS.reconfigure forSampling @08:22:58.48 SS.seek 25.00ml @08:22:59.27 Vacuum too high -- waiting up to 40s @08:22:59.53 Intake @48.1psia, vacuum from 20.0 to 24.0psi @08:22:59.54 SS.reconfigure forSampling @08:22:59.57 SS.seek 25.00ml @08:23:00.49 Vacuum too high -- waiting up to 40s @08:23:00.75 Intake @47.9psia, vacuum from 20.0 to 24.0psi @08:23:00.76 SS.reconfigure forSampling @08:23:00.79 SS.seek 25.00ml @08:23:01.80 Vacuum too high -- waiting up to 40s @08:23:02.06 Intake @47.9psia, vacuum from 20.0 to 24.0psi @08:23:02.07 SS.reconfigure forSampling @08:23:02.10 SS.seek 25.00ml @08:23:03.02 Vacuum too high -- waiting up to 40s @08:23:03.28 Intake @47.7psia, vacuum from 20.0 to 24.0psi @08:23:03.29 SS.reconfigure forSampling @08:23:03.33 SS.seek 25.00ml @08:23:04.33 Vacuum too high -- waiting up to 40s @08:23:04.59 SS.seek 25.00ml @08:23:05.46 Vacuum too high -- waiting up to 40s @08:23:05.71 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:23:05.72 SS.reconfigure forSampling @08:23:05.76 SS.seek 25.00ml @08:23:06.61 Vacuum too high -- waiting up to 40s @08:23:06.87 Intake @47.5psia, vacuum from 20.0 to 24.0psi @08:23:06.88 SS.reconfigure forSampling @08:23:06.91 SS.seek 25.00ml @08:23:07.80 Vacuum too high -- waiting up to 40s @08:23:08.06 Intake @47.3psia, vacuum from 20.0 to 24.0psi @08:23:08.07 SS.reconfigure forSampling @08:23:08.10 SS.seek 25.00ml @08:23:08.96 Vacuum too high -- waiting up to 40s @08:23:09.22 Intake @47.3psia, vacuum from 20.0 to 24.0psi @08:23:09.23 SS.reconfigure forSampling @08:23:09.26 SS.seek 25.00ml @08:23:10.21 Vacuum too high -- waiting up to 40s @08:23:10.47 Intake @47.7psia, vacuum from 20.0 to 24.0psi @08:23:10.48 SS.reconfigure forSampling @08:23:10.51 SS.seek 25.00ml @08:23:12.05 Vacuum too high -- waiting up to 40s @08:23:12.32 Intake @49.1psia, vacuum from 20.0 to 24.0psi @08:23:12.33 SS.reconfigure forSampling @08:23:12.37 SS.seek 25.00ml @08:23:13.77 Vacuum too high -- waiting up to 40s @08:23:14.03 Intake @48.3psia, vacuum from 20.0 to 24.0psi @08:23:14.04 SS.reconfigure forSampling @08:23:14.08 SS.seek 25.00ml @08:23:15.08 Vacuum too high -- waiting up to 40s @08:23:15.34 Intake @48.3psia, vacuum from 20.0 to 24.0psi @08:23:15.36 SS.reconfigure forSampling @08:23:15.39 SS.seek 25.00ml @08:23:16.30 Vacuum too high -- waiting up to 40s @08:23:16.56 Intake @48.0psia, vacuum from 20.0 to 24.0psi @08:23:16.57 SS.reconfigure forSampling @08:23:16.61 SS.seek 25.00ml @08:23:17.49 Vacuum too high -- waiting up to 40s @08:23:17.75 Intake @47.8psia, vacuum from 20.0 to 24.0psi @08:23:17.76 SS.reconfigure forSampling @08:23:17.80 SS.seek 25.00ml @08:23:18.71 Vacuum too high -- waiting up to 40s @08:23:18.98 Intake @47.9psia, vacuum from 20.0 to 24.0psi @08:23:18.99 SS.reconfigure forSampling @08:23:19.02 SS.seek 25.00ml @08:23:19.92 Vacuum too high -- waiting up to 40s @08:23:20.18 Intake @47.7psia, vacuum from 20.0 to 24.0psi @08:23:20.19 SS.reconfigure forSampling @08:23:20.23 SS.seek 25.00ml @08:23:21.14 Vacuum too high -- waiting up to 40s @08:23:21.40 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:23:21.41 SS.reconfigure forSampling @08:23:21.45 SS.seek 25.00ml @08:23:22.30 Vacuum too high -- waiting up to 40s @08:23:22.57 Intake @47.3psia, vacuum from 20.0 to 24.0psi @08:23:22.58 SS.reconfigure forSampling @08:23:22.61 SS.seek 25.00ml @08:23:23.49 Vacuum too high -- waiting up to 40s @08:23:23.75 Intake @47.5psia, vacuum from 20.0 to 24.0psi @08:23:23.76 SS.reconfigure forSampling @08:23:23.79 SS.seek 25.00ml @08:23:24.74 Vacuum too high -- waiting up to 40s @08:23:25.11 Intake @47.8psia, vacuum from 20.0 to 24.0psi @08:23:25.12 SS.reconfigure forSampling @08:23:25.15 SS.seek 25.00ml @08:23:26.21 Vacuum too high -- waiting up to 40s @08:23:26.47 Intake @48.2psia, vacuum from 20.0 to 24.0psi @08:23:26.48 SS.reconfigure forSampling @08:23:26.51 SS.seek 25.00ml @08:23:27.42 Vacuum too high -- waiting up to 40s @08:23:27.68 Intake @48.7psia, vacuum from 20.0 to 24.0psi @08:23:27.69 SS.reconfigure forSampling @08:23:27.73 SS.seek 25.00ml @08:23:28.58 Vacuum too high -- waiting up to 40s @08:23:28.86 Intake @48.1psia, vacuum from 20.0 to 24.0psi @08:23:28.87 SS.reconfigure forSampling @08:23:28.91 SS.seek 25.00ml @08:23:29.74 Vacuum too high -- waiting up to 40s @08:23:30.00 Intake @47.8psia, vacuum from 20.0 to 24.0psi @08:23:30.01 SS.reconfigure forSampling @08:23:30.04 SS.seek 25.00ml @08:23:30.92 Vacuum too high -- waiting up to 40s @08:23:31.18 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:23:31.19 SS.reconfigure forSampling @08:23:31.22 SS.seek 25.00ml @08:23:32.08 Vacuum too high -- waiting up to 40s @08:23:32.34 Intake @47.8psia, vacuum from 20.0 to 24.0psi @08:23:32.35 SS.reconfigure forSampling @08:23:32.38 SS.seek 25.00ml @08:23:33.21 Vacuum too high -- waiting up to 40s @08:23:33.47 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:23:33.48 SS.reconfigure forSampling @08:23:33.51 SS.seek 25.00ml @08:23:34.33 Vacuum too high -- waiting up to 40s @08:23:34.59 Intake @47.8psia, vacuum from 20.0 to 24.0psi @08:23:34.60 SS.reconfigure forSampling @08:23:34.64 SS.seek 25.00ml @08:23:35.46 Vacuum too high -- waiting up to 40s @08:23:35.73 SS.seek 25.00ml @08:23:36.49 Vacuum too high -- waiting up to 40s @08:23:36.75 Intake @47.7psia, vacuum from 20.0 to 24.0psi @08:23:36.76 SS.reconfigure forSampling @08:23:36.79 SS.seek 25.00ml @08:23:37.67 Vacuum too high -- waiting up to 40s @08:23:37.93 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:23:37.94 SS.reconfigure forSampling @08:23:37.97 SS.seek 25.00ml @08:23:38.77 Vacuum too high -- waiting up to 40s @08:23:39.03 Intake @47.2psia, vacuum from 20.0 to 24.0psi @08:23:39.04 SS.reconfigure forSampling @08:23:39.07 SS.seek 25.00ml @08:23:39.89 Vacuum too high -- waiting up to 40s @08:23:40.15 Intake @47.3psia, vacuum from 20.0 to 24.0psi @08:23:40.16 SS.reconfigure forSampling @08:23:40.19 SS.seek 25.00ml @08:23:41.02 Vacuum too high -- waiting up to 40s @08:23:42.03 Intake @49.0psia, vacuum from 20.0 to 24.0psi @08:23:42.04 SS.reconfigure forSampling @08:23:42.08 SS.seek 25.00ml @08:23:43.55 Vacuum too high -- waiting up to 40s @08:23:43.82 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:23:43.83 SS.reconfigure forSampling @08:23:43.86 SS.seek 25.00ml @08:23:44.80 Vacuum too high -- waiting up to 40s @08:23:45.06 Intake @47.7psia, vacuum from 20.0 to 24.0psi @08:23:45.07 SS.reconfigure forSampling @08:23:45.11 SS.seek 25.00ml @08:23:45.92 Vacuum too high -- waiting up to 40s @08:23:46.18 Intake @48.1psia, vacuum from 20.0 to 24.0psi @08:23:46.19 SS.reconfigure forSampling @08:23:46.23 SS.seek 25.00ml @08:23:47.08 Vacuum too high -- waiting up to 40s @08:23:47.57 Intake @48.8psia, vacuum from 20.0 to 24.0psi @08:23:47.58 SS.reconfigure forSampling @08:23:47.61 SS.seek 25.00ml @08:23:48.64 Vacuum too high -- waiting up to 40s @08:23:48.90 Intake @48.2psia, vacuum from 20.0 to 24.0psi @08:23:48.91 SS.reconfigure forSampling @08:23:48.95 SS.seek 25.00ml @08:23:49.80 Vacuum too high -- waiting up to 40s @08:23:50.06 Intake @47.9psia, vacuum from 20.0 to 24.0psi @08:23:50.07 SS.reconfigure forSampling @08:23:50.11 SS.seek 25.00ml @08:23:50.95 Vacuum too high -- waiting up to 40s @08:23:51.21 Intake @47.7psia, vacuum from 20.0 to 24.0psi @08:23:51.22 SS.reconfigure forSampling @08:23:51.26 SS.seek 25.00ml @08:23:52.02 Vacuum too high -- waiting up to 40s @08:23:52.28 Intake @47.5psia, vacuum from 20.0 to 24.0psi @08:23:52.29 SS.reconfigure forSampling @08:23:52.32 SS.seek 25.00ml @08:23:53.14 Vacuum too high -- waiting up to 40s @08:23:53.40 Intake @47.4psia, vacuum from 20.0 to 24.0psi @08:23:53.41 SS.reconfigure forSampling @08:23:53.45 SS.seek 25.00ml @08:23:54.24 Vacuum too high -- waiting up to 40s @08:23:54.50 Intake @47.5psia, vacuum from 20.0 to 24.0psi @08:23:54.51 SS.reconfigure forSampling @08:23:54.54 SS.seek 25.00ml @08:23:55.30 Vacuum too high -- waiting up to 40s @08:23:55.56 Intake @47.5psia, vacuum from 20.0 to 24.0psi @08:23:55.57 SS.reconfigure forSampling @08:23:55.61 SS.seek 25.00ml @08:23:56.42 Vacuum too high -- waiting up to 40s @08:23:56.68 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:23:56.69 SS.reconfigure forSampling @08:23:56.73 SS.seek 25.00ml @08:23:57.52 Vacuum too high -- waiting up to 40s @08:23:57.99 Intake @47.9psia, vacuum from 20.0 to 24.0psi @08:23:58.00 SS.reconfigure forSampling @08:23:58.04 SS.seek 25.00ml @08:23:59.02 Vacuum too high -- waiting up to 40s @08:23:59.28 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:23:59.29 SS.reconfigure forSampling @08:23:59.32 SS.seek 25.00ml @08:24:00.08 Vacuum too high -- waiting up to 40s @08:24:00.34 Intake @47.5psia, vacuum from 20.0 to 24.0psi @08:24:00.35 SS.reconfigure forSampling @08:24:00.38 SS.seek 25.00ml @08:24:01.17 Vacuum too high -- waiting up to 40s @08:24:01.43 Intake @47.8psia, vacuum from 20.0 to 24.0psi @08:24:01.44 SS.reconfigure forSampling @08:24:01.47 SS.seek 25.00ml @08:24:02.27 Vacuum too high -- waiting up to 40s @08:24:02.53 Intake @48.6psia, vacuum from 20.0 to 24.0psi @08:24:02.54 SS.reconfigure forSampling @08:24:02.57 SS.seek 25.00ml @08:24:03.24 Vacuum too high -- waiting up to 40s @08:24:03.50 Intake @48.2psia, vacuum from 20.0 to 24.0psi @08:24:03.51 SS.reconfigure forSampling @08:24:03.54 SS.seek 25.00ml @08:24:04.30 Vacuum too high -- waiting up to 40s @08:24:04.56 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:24:04.57 SS.reconfigure forSampling @08:24:04.61 SS.seek 25.00ml @08:24:05.27 Vacuum too high -- waiting up to 40s @08:24:05.54 SS.seek 25.00ml @08:24:06.24 Vacuum too high -- waiting up to 40s @08:24:06.50 Intake @47.9psia, vacuum from 20.0 to 24.0psi @08:24:06.51 SS.reconfigure forSampling @08:24:06.54 SS.seek 25.00ml @08:24:07.36 Vacuum too high -- waiting up to 40s @08:24:07.62 Intake @48.2psia, vacuum from 20.0 to 24.0psi @08:24:07.63 SS.reconfigure forSampling @08:24:07.67 SS.seek 25.00ml @08:24:08.42 Vacuum too high -- waiting up to 40s @08:24:08.68 Intake @48.2psia, vacuum from 20.0 to 24.0psi @08:24:08.69 SS.reconfigure forSampling @08:24:08.72 SS.seek 25.00ml @08:24:10.55 Vacuum too high -- waiting up to 40s @08:24:10.81 Intake @47.4psia, vacuum from 20.0 to 24.0psi @08:24:10.82 SS.reconfigure forSampling @08:24:10.85 SS.seek 25.00ml @08:24:11.67 Vacuum too high -- waiting up to 40s @08:24:11.93 Intake @47.3psia, vacuum from 20.0 to 24.0psi @08:24:11.94 SS.reconfigure forSampling @08:24:11.98 SS.seek 25.00ml @08:24:12.70 Vacuum too high -- waiting up to 40s @08:24:12.97 SS.seek 25.00ml @08:24:13.61 Vacuum too high -- waiting up to 40s @08:24:13.87 Intake @47.5psia, vacuum from 20.0 to 24.0psi @08:24:13.88 SS.reconfigure forSampling @08:24:13.92 SS.seek 25.00ml @08:24:14.70 Vacuum too high -- waiting up to 40s @08:24:14.96 Intake @47.2psia, vacuum from 20.0 to 24.0psi @08:24:14.97 SS.reconfigure forSampling @08:24:15.01 SS.seek 25.00ml @08:24:15.77 Vacuum too high -- waiting up to 40s @08:24:16.26 SS.seek 25.00ml @08:24:17.23 Vacuum too high -- waiting up to 40s @08:24:17.71 Intake @47.5psia, vacuum from 20.0 to 24.0psi @08:24:17.72 SS.reconfigure forSampling @08:24:17.75 SS.seek 25.00ml @08:24:18.70 Vacuum too high -- waiting up to 40s @08:24:18.96 Intake @47.7psia, vacuum from 20.0 to 24.0psi @08:24:18.97 SS.reconfigure forSampling @08:24:19.00 SS.seek 25.00ml @08:24:19.80 Vacuum too high -- waiting up to 40s @08:24:20.27 Intake @48.6psia, vacuum from 20.0 to 24.0psi @08:24:20.28 SS.reconfigure forSampling @08:24:20.32 SS.seek 25.00ml @08:24:21.27 Vacuum too high -- waiting up to 40s @08:24:21.53 Intake @48.0psia, vacuum from 20.0 to 24.0psi @08:24:21.54 SS.reconfigure forSampling @08:24:21.57 SS.seek 25.00ml @08:24:22.36 Vacuum too high -- waiting up to 40s @08:24:22.83 Intake @48.3psia, vacuum from 20.0 to 24.0psi @08:24:22.84 SS.reconfigure forSampling @08:24:22.87 SS.seek 25.00ml @08:24:23.70 Vacuum too high -- waiting up to 40s @08:24:23.96 Intake @48.1psia, vacuum from 20.0 to 24.0psi @08:24:23.97 SS.reconfigure forSampling @08:24:24.00 SS.seek 25.00ml @08:24:24.67 Vacuum too high -- waiting up to 40s @08:24:24.93 Intake @47.4psia, vacuum from 20.0 to 24.0psi @08:24:24.94 SS.reconfigure forSampling @08:24:24.97 SS.seek 25.00ml @08:24:25.70 Vacuum too high -- waiting up to 40s @08:24:25.96 Intake @47.3psia, vacuum from 20.0 to 24.0psi @08:24:25.97 SS.reconfigure forSampling @08:24:26.00 SS.seek 25.00ml @08:24:26.73 Vacuum too high -- waiting up to 40s @08:24:27.20 Intake @47.1psia, vacuum from 20.0 to 24.0psi @08:24:27.21 SS.reconfigure forSampling @08:24:27.24 SS.seek 25.00ml @08:24:28.27 Vacuum too high -- waiting up to 40s @08:24:28.75 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:24:28.76 SS.reconfigure forSampling @08:24:28.79 SS.seek 25.00ml @08:24:29.70 Vacuum too high -- waiting up to 40s @08:24:30.18 Intake @47.9psia, vacuum from 20.0 to 24.0psi @08:24:30.19 SS.reconfigure forSampling @08:24:30.22 SS.seek 25.00ml @08:24:31.08 Vacuum too high -- waiting up to 40s @08:24:31.34 Intake @47.9psia, vacuum from 20.0 to 24.0psi @08:24:31.35 SS.reconfigure forSampling @08:24:31.38 SS.seek 25.00ml @08:24:32.11 Vacuum too high -- waiting up to 40s @08:24:32.37 Intake @47.8psia, vacuum from 20.0 to 24.0psi @08:24:32.38 SS.reconfigure forSampling @08:24:32.41 SS.seek 25.00ml @08:24:33.17 Vacuum too high -- waiting up to 40s @08:24:33.43 Intake @47.5psia, vacuum from 20.0 to 24.0psi @08:24:33.44 SS.reconfigure forSampling @08:24:33.47 SS.seek 25.00ml @08:24:34.20 Vacuum too high -- waiting up to 40s @08:24:34.67 Intake @47.1psia, vacuum from 20.0 to 24.0psi @08:24:34.68 SS.reconfigure forSampling @08:24:34.71 SS.seek 25.00ml @08:24:35.70 Vacuum too high -- waiting up to 40s @08:24:36.18 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:24:36.19 SS.reconfigure forSampling @08:24:36.22 SS.seek 25.00ml @08:24:37.17 Vacuum too high -- waiting up to 40s @08:24:38.03 Intake @48.6psia, vacuum from 20.0 to 24.0psi @08:24:38.04 SS.reconfigure forSampling @08:24:38.07 SS.seek 25.00ml @08:24:39.23 Vacuum too high -- waiting up to 40s @08:24:39.49 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:24:39.50 SS.reconfigure forSampling @08:24:39.54 SS.seek 25.00ml @08:24:40.39 Vacuum too high -- waiting up to 40s @08:24:40.65 Intake @47.3psia, vacuum from 20.0 to 24.0psi @08:24:40.66 SS.reconfigure forSampling @08:24:40.70 SS.seek 25.00ml @08:24:41.48 Vacuum too high -- waiting up to 40s @08:24:41.95 Intake @47.8psia, vacuum from 20.0 to 24.0psi @08:24:41.96 SS.reconfigure forSampling @08:24:42.00 SS.seek 25.00ml @08:24:42.92 Vacuum too high -- waiting up to 40s @08:24:43.40 Intake @48.3psia, vacuum from 20.0 to 24.0psi @08:24:43.41 SS.reconfigure forSampling @08:24:43.45 SS.seek 25.00ml @08:24:44.50 Vacuum too high -- waiting up to 40s @08:24:44.77 Intake @48.2psia, vacuum from 20.0 to 24.0psi @08:24:44.78 SS.reconfigure forSampling @08:24:44.82 SS.seek 25.00ml @08:24:45.58 Vacuum too high -- waiting up to 40s @08:24:45.84 Intake @47.5psia, vacuum from 20.0 to 24.0psi @08:24:45.85 SS.reconfigure forSampling @08:24:45.89 SS.seek 25.00ml @08:24:46.64 Vacuum too high -- waiting up to 40s @08:24:47.13 SS.seek 25.00ml @08:24:48.08 Vacuum too high -- waiting up to 40s @08:24:48.35 SS.seek 25.00ml @08:24:49.01 Vacuum too high -- waiting up to 40s @08:24:49.48 Intake @47.4psia, vacuum from 20.0 to 24.0psi @08:24:49.49 SS.reconfigure forSampling @08:24:49.52 SS.seek 25.00ml @08:24:50.36 Vacuum too high -- waiting up to 40s @08:24:50.83 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:24:50.84 SS.reconfigure forSampling @08:24:50.87 SS.seek 25.00ml @08:24:51.70 Vacuum too high -- waiting up to 40s @08:24:52.17 Intake @47.8psia, vacuum from 20.0 to 24.0psi @08:24:52.18 SS.reconfigure forSampling @08:24:52.22 SS.seek 25.00ml @08:24:53.17 Vacuum too high -- waiting up to 40s @08:24:53.65 Intake @47.9psia, vacuum from 20.0 to 24.0psi @08:24:53.66 SS.reconfigure forSampling @08:24:53.70 SS.seek 25.00ml @08:24:54.76 Vacuum too high -- waiting up to 40s @08:24:55.23 Intake @48.0psia, vacuum from 20.0 to 24.0psi @08:24:55.24 SS.reconfigure forSampling @08:24:55.28 SS.seek 25.00ml @08:24:56.23 Vacuum too high -- waiting up to 40s @08:24:56.70 Intake @48.0psia, vacuum from 20.0 to 24.0psi @08:24:56.71 SS.reconfigure forSampling @08:24:56.75 SS.seek 25.00ml @08:24:57.40 Stroke completed -- waiting for pressure to equalize ... @08:24:59.20 Intake.select :close @08:24:59.35 SSV.dial :exhaust @08:25:00.13 Exhaust.select :open @08:25:00.22 SS.reconfigure SSexhale @08:25:00.26 SS.seek 0.00ml @08:25:09.37 Exhaust.select :close @08:25:09.46 SSV.dial CBV @08:25:09.48
CBV.dial SSV @08:25:10.27 Allowing 185s for 25.0ml of remaining 850.0ml @08:25:10.28 Intake.select :open @08:25:12.41 Intake @48.0psia, vacuum from 20.0 to 24.0psi @08:25:12.42 SS.reconfigure forSampling @08:25:12.46 SS.seek 25.00ml @08:25:13.92 Vacuum too high -- waiting up to 40s @08:25:14.18 Intake @47.3psia, vacuum from 20.0 to 24.0psi @08:25:14.19 SS.reconfigure forSampling @08:25:14.22 SS.seek 25.00ml @08:25:14.95 Vacuum too high -- waiting up to 40s @08:25:15.21 Intake @47.4psia, vacuum from 20.0 to 24.0psi @08:25:15.22 SS.reconfigure forSampling @08:25:15.25 SS.seek 25.00ml @08:25:15.98 Vacuum too high -- waiting up to 40s @08:25:16.24 Intake @47.4psia, vacuum from 20.0 to 24.0psi @08:25:16.25 SS.reconfigure forSampling @08:25:16.28 SS.seek 25.00ml @08:25:17.01 Vacuum too high -- waiting up to 40s @08:25:17.27 Intake @47.4psia, vacuum from 20.0 to 24.0psi @08:25:17.28 SS.reconfigure forSampling @08:25:17.31 SS.seek 25.00ml @08:25:18.61 Vacuum too high -- waiting up to 40s @08:25:18.87 Intake @47.2psia, vacuum from 20.0 to 24.0psi @08:25:18.88 SS.reconfigure forSampling @08:25:18.92 SS.seek 25.00ml @08:25:20.17 Vacuum too high -- waiting up to 40s @08:25:20.43 Intake @47.4psia, vacuum from 20.0 to 24.0psi @08:25:20.44 SS.reconfigure forSampling @08:25:20.47 SS.seek 25.00ml @08:25:21.23 Vacuum too high -- waiting up to 40s @08:25:21.49 Intake @47.9psia, vacuum from 20.0 to 24.0psi @08:25:21.50 SS.reconfigure forSampling @08:25:21.54 SS.seek 25.00ml @08:25:22.26 Vacuum too high -- waiting up to 40s @08:25:22.52 Intake @48.6psia, vacuum from 20.0 to 24.0psi @08:25:22.54 SS.reconfigure forSampling @08:25:22.57 SS.seek 25.00ml @08:25:23.17 Vacuum too high -- waiting up to 40s @08:25:23.43 Intake @48.5psia, vacuum from 20.0 to 24.0psi @08:25:23.44 SS.reconfigure forSampling @08:25:23.48 SS.seek 25.00ml @08:25:24.49 Vacuum too high -- waiting up to 40s @08:25:24.75 Intake @47.8psia, vacuum from 20.0 to 24.0psi @08:25:24.76 SS.reconfigure forSampling @08:25:24.80 SS.seek 25.00ml @08:25:25.98 Vacuum too high -- waiting up to 40s @08:25:26.45 Intake @48.7psia, vacuum from 20.0 to 24.0psi @08:25:26.46 SS.reconfigure forSampling @08:25:26.49 SS.seek 25.00ml @08:25:27.32 Vacuum too high -- waiting up to 40s @08:25:27.58 Intake @47.7psia, vacuum from 20.0 to 24.0psi @08:25:27.59 SS.reconfigure forSampling @08:25:27.62 SS.seek 25.00ml @08:25:28.36 Vacuum too high -- waiting up to 40s @08:25:28.62 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:25:28.63 SS.reconfigure forSampling @08:25:28.67 SS.seek 25.00ml @08:25:29.32 Vacuum too high -- waiting up to 40s @08:25:29.58 Intake @47.5psia, vacuum from 20.0 to 24.0psi @08:25:29.59 SS.reconfigure forSampling @08:25:29.63 SS.seek 25.00ml @08:25:30.29 Vacuum too high -- waiting up to 40s @08:25:30.55 Intake @47.4psia, vacuum from 20.0 to 24.0psi @08:25:30.56 SS.reconfigure forSampling @08:25:30.60 SS.seek 25.00ml @08:25:31.23 Vacuum too high -- waiting up to 40s @08:25:31.49 Intake @47.3psia, vacuum from 20.0 to 24.0psi @08:25:31.50 SS.reconfigure forSampling @08:25:31.54 SS.seek 25.00ml @08:25:32.17 Vacuum too high -- waiting up to 40s @08:25:32.44 SS.seek 25.00ml @08:25:33.11 Vacuum too high -- waiting up to 40s @08:25:33.37 Intake @47.3psia, vacuum from 20.0 to 24.0psi @08:25:33.38 SS.reconfigure forSampling @08:25:33.41 SS.seek 25.00ml @08:25:34.04 Vacuum too high -- waiting up to 40s @08:25:34.30 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:25:34.31 SS.reconfigure forSampling @08:25:34.35 SS.seek 25.00ml @08:25:34.95 Vacuum too high -- waiting up to 40s @08:25:35.21 Intake @47.8psia, vacuum from 20.0 to 24.0psi @08:25:35.22 SS.reconfigure forSampling @08:25:35.26 SS.seek 25.00ml @08:25:35.95 Vacuum too high -- waiting up to 40s @08:25:36.21 Intake @48.1psia, vacuum from 20.0 to 24.0psi @08:25:36.22 SS.reconfigure forSampling @08:25:36.25 SS.seek 25.00ml @08:25:36.92 Vacuum too high -- waiting up to 40s @08:25:37.40 Intake @49.1psia, vacuum from 20.0 to 24.0psi @08:25:37.41 SS.reconfigure forSampling @08:25:37.44 SS.seek 25.00ml @08:25:38.29 Vacuum too high -- waiting up to 40s @08:25:38.55 Intake @48.6psia, vacuum from 20.0 to 24.0psi @08:25:38.56 SS.reconfigure forSampling @08:25:38.60 SS.seek 25.00ml @08:25:39.23 Vacuum too high -- waiting up to 40s @08:25:39.50 Intake @48.0psia, vacuum from 20.0 to 24.0psi @08:25:39.51 SS.reconfigure forSampling @08:25:39.54 SS.seek 25.00ml @08:25:40.26 Vacuum too high -- waiting up to 40s @08:25:40.52 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:25:40.53 SS.reconfigure forSampling @08:25:40.56 SS.seek 25.00ml @08:25:41.20 Vacuum too high -- waiting up to 40s @08:25:41.46 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:25:41.47 SS.reconfigure forSampling @08:25:41.51 SS.seek 25.00ml @08:25:42.17 Vacuum too high -- waiting up to 40s @08:25:42.43 Intake @47.4psia, vacuum from 20.0 to 24.0psi @08:25:42.44 SS.reconfigure forSampling @08:25:42.47 SS.seek 25.00ml @08:25:43.10 Vacuum too high -- waiting up to 40s @08:25:43.36 Intake @47.5psia, vacuum from 20.0 to 24.0psi @08:25:43.37 SS.reconfigure forSampling @08:25:43.41 SS.seek 25.00ml @08:25:45.14 Vacuum too high -- waiting up to 40s @08:25:45.40 Intake @47.3psia, vacuum from 20.0 to 24.0psi @08:25:45.41 SS.reconfigure forSampling @08:25:45.45 SS.seek 25.00ml @08:25:46.14 Vacuum too high -- waiting up to 40s @08:25:46.41 SS.seek 25.00ml @08:25:47.01 Vacuum too high -- waiting up to 40s @08:25:47.28 SS.seek 25.00ml @08:25:47.95 Vacuum too high -- waiting up to 40s @08:25:48.21 Intake @47.5psia, vacuum from 20.0 to 24.0psi @08:25:48.22 SS.reconfigure forSampling @08:25:48.26 SS.seek 25.00ml @08:25:48.92 Vacuum too high -- waiting up to 40s @08:25:49.40 Intake @48.9psia, vacuum from 20.0 to 24.0psi @08:25:49.41 SS.reconfigure forSampling @08:25:49.45 SS.seek 25.00ml @08:25:50.23 Vacuum too high -- waiting up to 40s @08:25:50.49 Intake @48.9psia, vacuum from 20.0 to 24.0psi @08:25:50.50 SS.reconfigure forSampling @08:25:50.53 SS.seek 25.00ml @08:25:51.14 Vacuum too high -- waiting up to 40s @08:25:51.40 Intake @47.9psia, vacuum from 20.0 to 24.0psi @08:25:51.41 SS.reconfigure forSampling @08:25:51.44 SS.seek 25.00ml @08:25:52.14 Vacuum too high -- waiting up to 40s @08:25:52.63 SS.seek 25.00ml @08:25:53.54 Vacuum too high -- waiting up to 40s @08:25:54.02 Intake @47.8psia, vacuum from 20.0 to 24.0psi @08:25:54.03 SS.reconfigure forSampling @08:25:54.06 SS.seek 25.00ml @08:25:55.04 Vacuum too high -- waiting up to 40s @08:25:55.30 Intake @47.3psia, vacuum from 20.0 to 24.0psi @08:25:55.31 SS.reconfigure forSampling @08:25:55.35 SS.seek 25.00ml @08:25:55.98 Vacuum too high -- waiting up to 40s @08:25:56.24 Intake @47.4psia, vacuum from 20.0 to 24.0psi @08:25:56.25 SS.reconfigure forSampling @08:25:56.28 SS.seek 25.00ml @08:25:56.82 Vacuum too high -- waiting up to 40s @08:25:57.08 Intake @47.2psia, vacuum from 20.0 to 24.0psi @08:25:57.09 SS.reconfigure forSampling @08:25:57.13 SS.seek 25.00ml @08:25:57.76 Vacuum too high -- waiting up to 40s @08:25:58.02 Intake @47.2psia, vacuum from 20.0 to 24.0psi @08:25:58.03 SS.reconfigure forSampling @08:25:58.07 SS.seek 25.00ml @08:25:58.70 Vacuum too high -- waiting up to 40s @08:25:58.96 Intake @47.0psia, vacuum from 20.0 to 24.0psi @08:25:58.97 SS.reconfigure forSampling @08:25:59.01 SS.seek 25.00ml @08:25:59.67 Vacuum too high -- waiting up to 40s @08:26:00.14 Intake @46.9psia, vacuum from 20.0 to 24.0psi @08:26:00.15 SS.reconfigure forSampling @08:26:00.18 SS.seek 25.00ml @08:26:01.04 Vacuum too high -- waiting up to 40s @08:26:01.52 Intake @46.9psia, vacuum from 20.0 to 24.0psi @08:26:01.53 SS.reconfigure forSampling @08:26:01.56 SS.seek 25.00ml @08:26:02.42 Vacuum too high -- waiting up to 40s @08:26:02.68 Intake @46.9psia, vacuum from 20.0 to 24.0psi @08:26:02.69 SS.reconfigure forSampling @08:26:02.72 SS.seek 25.00ml @08:26:03.32 Vacuum too high -- waiting up to 40s @08:26:04.47 Intake @46.9psia, vacuum from 20.0 to 24.0psi @08:26:04.48 SS.reconfigure forSampling @08:26:04.52 SS.seek 25.00ml @08:26:05.95 Vacuum too high -- waiting up to 40s @08:26:06.42 Intake @48.0psia, vacuum from 20.0 to 24.0psi @08:26:06.43 SS.reconfigure forSampling @08:26:06.46 SS.seek 25.00ml @08:26:07.29 Vacuum too high -- waiting up to 40s @08:26:07.55 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:26:07.56 SS.reconfigure forSampling @08:26:07.59 SS.seek 25.00ml @08:26:08.23 Vacuum too high -- waiting up to 40s @08:26:08.49 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:26:08.50 SS.reconfigure forSampling @08:26:08.53 SS.seek 25.00ml @08:26:09.10 Vacuum too high -- waiting up to 40s @08:26:09.36 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:26:09.37 SS.reconfigure forSampling @08:26:09.40 SS.seek 25.00ml @08:26:09.95 Vacuum too high -- waiting up to 40s @08:26:10.42 Intake @47.9psia, vacuum from 20.0 to 24.0psi @08:26:10.43 SS.reconfigure forSampling @08:26:10.46 SS.seek 25.00ml @08:26:12.23 Vacuum too high -- waiting up to 40s @08:26:12.49 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:26:12.50 SS.reconfigure forSampling @08:26:12.54 SS.seek 25.00ml @08:26:13.20 Vacuum too high -- waiting up to 40s @08:26:13.46 Intake @47.4psia, vacuum from 20.0 to 24.0psi @08:26:13.47 SS.reconfigure forSampling @08:26:13.51 SS.seek 25.00ml @08:26:14.17 Vacuum too high -- waiting up to 40s @08:26:14.65 Intake @47.2psia, vacuum from 20.0 to 24.0psi @08:26:14.66 SS.reconfigure forSampling @08:26:14.70 SS.seek 25.00ml @08:26:15.54 Vacuum too high -- waiting up to 40s @08:26:16.03 SS.seek 25.00ml @08:26:16.82 Vacuum too high -- waiting up to 40s @08:26:17.30 Intake @47.4psia, vacuum from 20.0 to 24.0psi @08:26:17.31 SS.reconfigure forSampling @08:26:17.35 SS.seek 25.00ml @08:26:18.13 Vacuum too high -- waiting up to 40s @08:26:18.61 Intake @48.4psia, vacuum from 20.0 to 24.0psi @08:26:18.62 SS.reconfigure forSampling @08:26:18.66 SS.seek 25.00ml @08:26:19.45 Vacuum too high -- waiting up to 40s @08:26:19.71 Intake @47.9psia, vacuum from 20.0 to 24.0psi @08:26:19.72 SS.reconfigure forSampling @08:26:19.75 SS.seek 25.00ml @08:26:20.32 Vacuum too high -- waiting up to 40s @08:26:20.58 Intake @47.5psia, vacuum from 20.0 to 24.0psi @08:26:20.59 SS.reconfigure forSampling @08:26:20.62 SS.seek 25.00ml @08:26:21.26 Vacuum too high -- waiting up to 40s @08:26:21.52 Intake @47.2psia, vacuum from 20.0 to 24.0psi @08:26:21.53 SS.reconfigure forSampling @08:26:21.56 SS.seek 25.00ml @08:26:22.23 Vacuum too high -- waiting up to 40s @08:26:22.71 Intake @47.1psia, vacuum from 20.0 to 24.0psi @08:26:22.72 SS.reconfigure forSampling @08:26:22.75 SS.seek 25.00ml @08:26:23.54 Vacuum too high -- waiting up to 40s @08:26:24.02 Intake @47.3psia, vacuum from 20.0 to 24.0psi @08:26:24.03 SS.reconfigure forSampling @08:26:24.06 SS.seek 25.00ml @08:26:24.88 Vacuum too high -- waiting up to 40s @08:26:25.14 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:26:25.15 SS.reconfigure forSampling @08:26:25.18 SS.seek 25.00ml @08:26:25.73 Vacuum too high -- waiting up to 40s @08:26:26.20 Intake @47.9psia, vacuum from 20.0 to 24.0psi @08:26:26.21 SS.reconfigure forSampling @08:26:26.24 SS.seek 25.00ml @08:26:27.04 Vacuum too high -- waiting up to 40s @08:26:27.52 Intake @48.2psia, vacuum from 20.0 to 24.0psi @08:26:27.53 SS.reconfigure forSampling @08:26:27.56 SS.seek 25.00ml @08:26:28.42 Vacuum too high -- waiting up to 40s @08:26:28.68 Intake @47.8psia, vacuum from 20.0 to 24.0psi @08:26:28.69 SS.reconfigure forSampling @08:26:28.73 SS.seek 25.00ml @08:26:29.35 Vacuum too high -- waiting up to 40s @08:26:29.83 Intake @47.8psia, vacuum from 20.0 to 24.0psi @08:26:29.84 SS.reconfigure forSampling @08:26:29.87 SS.seek 25.00ml @08:26:30.73 Vacuum too high -- waiting up to 40s @08:26:31.21 Intake @47.8psia, vacuum from 20.0 to 24.0psi @08:26:31.22 SS.reconfigure forSampling @08:26:31.25 SS.seek 25.00ml @08:26:32.07 Vacuum too high -- waiting up to 40s @08:26:32.55 Intake @47.5psia, vacuum from 20.0 to 24.0psi @08:26:32.56 SS.reconfigure forSampling @08:26:32.59 SS.seek 25.00ml @08:26:33.48 Vacuum too high -- waiting up to 40s @08:26:33.94 Intake @47.7psia, vacuum from 20.0 to 24.0psi @08:26:33.95 SS.reconfigure forSampling @08:26:33.99 SS.seek 25.00ml @08:26:34.79 Vacuum too high -- waiting up to 40s @08:26:35.26 SS.seek 25.00ml @08:26:35.98 Vacuum too high -- waiting up to 40s @08:26:36.46 Intake @47.8psia, vacuum from 20.0 to 24.0psi @08:26:36.47 SS.reconfigure forSampling @08:26:36.51 SS.seek 25.00ml @08:26:38.23 Vacuum too high -- waiting up to 40s @08:26:38.71 Intake @47.9psia, vacuum from 20.0 to 24.0psi @08:26:38.73 SS.reconfigure forSampling @08:26:38.76 SS.seek 25.00ml @08:26:39.54 Vacuum too high -- waiting up to 40s @08:26:39.80 Intake @47.7psia, vacuum from 20.0 to 24.0psi @08:26:39.81 SS.reconfigure forSampling @08:26:39.85 SS.seek 25.00ml @08:26:40.45 Vacuum too high -- waiting up to 40s @08:26:40.93 Intake @47.4psia, vacuum from 20.0 to 24.0psi @08:26:40.94 SS.reconfigure forSampling @08:26:40.97 SS.seek 25.00ml @08:26:41.82 Vacuum too high -- waiting up to 40s @08:26:42.29 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:26:42.30 SS.reconfigure forSampling @08:26:42.33 SS.seek 25.00ml @08:26:43.04 Vacuum too high -- waiting up to 40s @08:26:43.51 Intake @47.7psia, vacuum from 20.0 to 24.0psi @08:26:43.52 SS.reconfigure forSampling @08:26:43.55 SS.seek 25.00ml @08:26:44.35 Vacuum too high -- waiting up to 40s @08:26:44.82 Intake @48.0psia, vacuum from 20.0 to 24.0psi @08:26:44.83 SS.reconfigure forSampling @08:26:44.87 SS.seek 25.00ml @08:26:45.73 Vacuum too high -- waiting up to 40s @08:26:46.21 Intake @48.6psia, vacuum from 20.0 to 24.0psi @08:26:46.22 SS.reconfigure forSampling @08:26:46.25 SS.seek 25.00ml @08:26:47.01 Vacuum too high -- waiting up to 40s @08:26:47.48 Intake @47.7psia, vacuum from 20.0 to 24.0psi @08:26:47.49 SS.reconfigure forSampling @08:26:47.52 SS.seek 25.00ml @08:26:48.32 Vacuum too high -- waiting up to 40s @08:26:48.80 Intake @47.4psia, vacuum from 20.0 to 24.0psi @08:26:48.81 SS.reconfigure forSampling @08:26:48.85 SS.seek 25.00ml @08:26:49.63 Vacuum too high -- waiting up to 40s @08:26:50.11 Intake @47.3psia, vacuum from 20.0 to 24.0psi @08:26:50.12 SS.reconfigure forSampling @08:26:50.15 SS.seek 25.00ml @08:26:50.95 Vacuum too high -- waiting up to 40s @08:26:51.43 Intake @47.3psia, vacuum from 20.0 to 24.0psi @08:26:51.44 SS.reconfigure forSampling @08:26:51.47 SS.seek 25.00ml @08:26:52.26 Vacuum too high -- waiting up to 40s @08:26:52.74 Intake @47.1psia, vacuum from 20.0 to 24.0psi @08:26:52.75 SS.reconfigure forSampling @08:26:52.78 SS.seek 25.00ml @08:26:53.66 Vacuum too high -- waiting up to 40s @08:26:54.13 Intake @46.9psia, vacuum from 20.0 to 24.0psi @08:26:54.14 SS.reconfigure forSampling @08:26:54.17 SS.seek 25.00ml @08:26:54.95 Vacuum too high -- waiting up to 40s @08:26:55.43 Intake @47.0psia, vacuum from 20.0 to 24.0psi @08:26:55.44 SS.reconfigure forSampling @08:26:55.47 SS.seek 25.00ml @08:26:56.23 Vacuum too high -- waiting up to 40s @08:26:56.71 Intake @47.8psia, vacuum from 20.0 to 24.0psi @08:26:56.72 SS.reconfigure forSampling @08:26:56.75 SS.seek 25.00ml @08:26:57.57 Vacuum too high -- waiting up to 40s @08:26:58.04 Intake @47.9psia, vacuum from 20.0 to 24.0psi @08:26:58.05 SS.reconfigure forSampling @08:26:58.08 SS.seek 25.00ml @08:26:58.74 Vacuum too high -- waiting up to 40s @08:26:59.00 Intake @47.9psia, vacuum from 20.0 to 24.0psi @08:26:59.01 SS.reconfigure forSampling @08:26:59.04 SS.seek 25.00ml @08:26:59.60 Vacuum too high -- waiting up to 40s @08:27:00.08 Intake @47.8psia, vacuum from 20.0 to 24.0psi @08:27:00.09 SS.reconfigure forSampling @08:27:00.12 SS.seek 25.00ml @08:27:00.91 Vacuum too high -- waiting up to 40s @08:27:01.39 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:27:01.40 SS.reconfigure forSampling @08:27:01.43 SS.seek 25.00ml @08:27:02.19 Vacuum too high -- waiting up to 40s @08:27:02.66 Intake @47.8psia, vacuum from 20.0 to 24.0psi @08:27:03.28 SS.reconfigure forSampling @08:27:03.31 SS.seek 25.00ml @08:27:04.51 Vacuum too high -- waiting up to 40s @08:27:04.99 Intake @48.0psia, vacuum from 20.0 to 24.0psi @08:27:05.00 SS.reconfigure forSampling @08:27:05.04 SS.seek 25.00ml @08:27:05.85 Vacuum too high -- waiting up to 40s @08:27:06.32 Intake @47.3psia, vacuum from 20.0 to 24.0psi @08:27:06.33 SS.reconfigure forSampling @08:27:06.37 SS.seek 25.00ml @08:27:07.26 Vacuum too high -- waiting up to 40s @08:27:07.74 Intake @47.2psia, vacuum from 20.0 to 24.0psi @08:27:07.75 SS.reconfigure forSampling @08:27:07.79 SS.seek 25.00ml @08:27:08.60 Vacuum too high -- waiting up to 40s @08:27:09.08 Intake @47.0psia, vacuum from 20.0 to 24.0psi @08:27:09.09 SS.reconfigure forSampling @08:27:09.12 SS.seek 25.00ml @08:27:09.88 Vacuum too high -- waiting up to 40s @08:27:10.35 Intake @47.1psia, vacuum from 20.0 to 24.0psi @08:27:10.36 SS.reconfigure forSampling @08:27:10.39 SS.seek 25.00ml @08:27:11.19 Vacuum too high -- waiting up to 40s @08:27:11.66 Intake @47.0psia, vacuum from 20.0 to 24.0psi @08:27:11.67 SS.reconfigure forSampling @08:27:11.71 SS.seek 25.00ml @08:27:12.51 Vacuum too high -- waiting up to 40s @08:27:12.98 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:27:12.99 SS.reconfigure forSampling @08:27:13.03 SS.seek 25.00ml @08:27:13.73 Vacuum too high -- waiting up to 40s @08:27:14.22 Intake @48.2psia, vacuum from 20.0 to 24.0psi @08:27:14.23 SS.reconfigure forSampling @08:27:14.26 SS.seek 25.00ml @08:27:15.01 Vacuum too high -- waiting up to 40s @08:27:15.50 SS.seek 25.00ml @08:27:16.29 Vacuum too high -- waiting up to 40s @08:27:16.77 Intake @48.9psia, vacuum from 20.0 to 24.0psi @08:27:16.78 SS.reconfigure forSampling @08:27:16.81 SS.seek 25.00ml @08:27:17.57 Vacuum too high -- waiting up to 40s @08:27:18.04 Intake @48.2psia, vacuum from 20.0 to 24.0psi @08:27:18.05 SS.reconfigure forSampling @08:27:18.09 SS.seek 25.00ml @08:27:18.82 Vacuum too high -- waiting up to 40s @08:27:19.30 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:27:19.31 SS.reconfigure forSampling @08:27:19.35 SS.seek 25.00ml @08:27:20.10 Vacuum too high -- waiting up to 40s @08:27:20.58 Intake @47.8psia, vacuum from 20.0 to 24.0psi @08:27:20.59 SS.reconfigure forSampling @08:27:20.62 SS.seek 25.00ml @08:27:21.41 Vacuum too high -- waiting up to 40s @08:27:21.89 Intake @47.7psia, vacuum from 20.0 to 24.0psi @08:27:21.90 SS.reconfigure forSampling @08:27:21.93 SS.seek 25.00ml @08:27:22.69 Vacuum too high -- waiting up to 40s @08:27:23.16 Intake @47.7psia, vacuum from 20.0 to 24.0psi @08:27:23.17 SS.reconfigure forSampling @08:27:23.20 SS.seek 25.00ml @08:27:23.91 Vacuum too high -- waiting up to 40s @08:27:24.38 Intake @47.5psia, vacuum from 20.0 to 24.0psi @08:27:24.39 SS.reconfigure forSampling @08:27:24.42 SS.seek 25.00ml @08:27:25.19 Vacuum too high -- waiting up to 40s @08:27:25.67 Intake @47.0psia, vacuum from 20.0 to 24.0psi @08:27:25.68 SS.reconfigure forSampling @08:27:25.71 SS.seek 25.00ml @08:27:26.47 Vacuum too high -- waiting up to 40s @08:27:26.95 Intake @47.2psia, vacuum from 20.0 to 24.0psi @08:27:26.96 SS.reconfigure forSampling @08:27:26.99 SS.seek 25.00ml @08:27:27.66 Vacuum too high -- waiting up to 40s @08:27:28.13 Intake @47.2psia, vacuum from 20.0 to 24.0psi @08:27:28.14 SS.reconfigure forSampling @08:27:28.17 SS.seek 25.00ml @08:27:29.01 Vacuum too high -- waiting up to 40s @08:27:30.09 Intake @47.9psia, vacuum from 20.0 to 24.0psi @08:27:30.10 SS.reconfigure forSampling @08:27:30.13 SS.seek 25.00ml @08:27:31.26 Vacuum too high -- waiting up to 40s @08:27:31.74 Intake @49.2psia, vacuum from 20.0 to 24.0psi @08:27:31.75 SS.reconfigure forSampling @08:27:31.78 SS.seek 25.00ml @08:27:32.47 Vacuum too high -- waiting up to 40s @08:27:32.73 Intake @48.2psia, vacuum from 20.0 to 24.0psi @08:27:32.74 SS.reconfigure forSampling @08:27:32.78 SS.seek 25.00ml @08:27:33.38 Vacuum too high -- waiting up to 40s @08:27:33.87 Intake @48.5psia, vacuum from 20.0 to 24.0psi @08:27:33.88 SS.reconfigure forSampling @08:27:33.91 SS.seek 25.00ml @08:27:34.66 Vacuum too high -- waiting up to 40s @08:27:35.14 Intake @47.9psia, vacuum from 20.0 to 24.0psi @08:27:35.15 SS.reconfigure forSampling @08:27:35.18 SS.seek 25.00ml @08:27:35.88 Vacuum too high -- waiting up to 40s @08:27:36.35 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:27:36.36 SS.reconfigure forSampling @08:27:36.39 SS.seek 25.00ml @08:27:37.19 Vacuum too high -- waiting up to 40s @08:27:37.66 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:27:37.67 SS.reconfigure forSampling @08:27:37.71 SS.seek 25.00ml @08:27:38.47 Vacuum too high -- waiting up to 40s @08:27:38.95 Intake @47.8psia, vacuum from 20.0 to 24.0psi @08:27:38.96 SS.reconfigure forSampling @08:27:38.99 SS.seek 25.00ml @08:27:39.69 Vacuum too high -- waiting up to 40s @08:27:40.17 Intake @47.5psia, vacuum from 20.0 to 24.0psi @08:27:40.18 SS.reconfigure forSampling @08:27:40.21 SS.seek 25.00ml @08:27:40.85 Vacuum too high -- waiting up to 40s @08:27:41.33 Intake @47.2psia, vacuum from 20.0 to 24.0psi @08:27:41.34 SS.reconfigure forSampling @08:27:41.37 SS.seek 25.00ml @08:27:42.10 Vacuum too high -- waiting up to 40s @08:27:42.58 Intake @47.2psia, vacuum from 20.0 to 24.0psi @08:27:42.59 SS.reconfigure forSampling @08:27:42.62 SS.seek 25.00ml @08:27:43.32 Vacuum too high -- waiting up to 40s @08:27:43.81 SS.seek 25.00ml @08:27:44.44 Vacuum too high -- waiting up to 40s @08:27:44.92 Intake @47.3psia, vacuum from 20.0 to 24.0psi @08:27:44.93 SS.reconfigure forSampling @08:27:44.96 SS.seek 25.00ml @08:27:45.69 Vacuum too high -- waiting up to 40s @08:27:46.16 Intake @47.9psia, vacuum from 20.0 to 24.0psi @08:27:46.17 SS.reconfigure forSampling @08:27:46.20 SS.seek 25.00ml @08:27:47.00 Vacuum too high -- waiting up to 40s @08:27:47.48 Intake @48.8psia, vacuum from 20.0 to 24.0psi @08:27:47.49 SS.reconfigure forSampling @08:27:47.52 SS.seek 25.00ml @08:27:48.25 Vacuum too high -- waiting up to 40s @08:27:48.73 Intake @47.8psia, vacuum from 20.0 to 24.0psi @08:27:48.74 SS.reconfigure forSampling @08:27:48.77 SS.seek 25.00ml @08:27:49.54 Vacuum too high -- waiting up to 40s @08:27:50.01 Intake @47.5psia, vacuum from 20.0 to 24.0psi @08:27:50.02 SS.reconfigure forSampling @08:27:50.05 SS.seek 25.00ml @08:27:50.82 Vacuum too high -- waiting up to 40s @08:27:51.29 Intake @47.8psia, vacuum from 20.0 to 24.0psi @08:27:51.30 SS.reconfigure forSampling @08:27:51.33 SS.seek 25.00ml @08:27:51.94 Vacuum too high -- waiting up to 40s @08:27:52.42 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:27:52.43 SS.reconfigure forSampling @08:27:52.46 SS.seek 25.00ml @08:27:53.25 Vacuum too high -- waiting up to 40s @08:27:53.72 SS.seek 25.00ml @08:27:54.41 Vacuum too high -- waiting up to 40s @08:27:55.22 Intake @47.3psia, vacuum from 20.0 to 24.0psi @08:27:55.23 SS.reconfigure forSampling @08:27:55.27 SS.seek 25.00ml @08:27:56.22 Vacuum too high -- waiting up to 40s @08:27:56.69 Intake @47.2psia, vacuum from 20.0 to 24.0psi @08:27:56.70 SS.reconfigure forSampling @08:27:56.74 SS.seek 25.00ml @08:27:57.50 Vacuum too high -- waiting up to 40s @08:27:57.97 Intake @47.2psia, vacuum from 20.0 to 24.0psi @08:27:57.99 SS.reconfigure forSampling @08:27:58.02 SS.seek 25.00ml @08:27:58.72 Vacuum too high -- waiting up to 40s @08:27:59.19 Intake @47.4psia, vacuum from 20.0 to 24.0psi @08:27:59.20 SS.reconfigure forSampling @08:27:59.23 SS.seek 25.00ml @08:27:59.94 Vacuum too high -- waiting up to 40s @08:28:00.41 Intake @48.3psia, vacuum from 20.0 to 24.0psi @08:28:00.42 SS.reconfigure forSampling @08:28:00.45 SS.seek 25.00ml @08:28:01.13 Vacuum too high -- waiting up to 40s @08:28:01.60 Intake @48.5psia, vacuum from 20.0 to 24.0psi @08:28:01.61 SS.reconfigure forSampling @08:28:01.64 SS.seek 25.00ml @08:28:02.29 Vacuum too high -- waiting up to 40s @08:28:02.76 Intake @47.9psia, vacuum from 20.0 to 24.0psi @08:28:02.77 SS.reconfigure forSampling @08:28:02.81 SS.seek 25.00ml @08:28:03.47 Vacuum too high -- waiting up to 40s @08:28:03.95 SS.seek 25.00ml @08:28:04.69 Vacuum too high -- waiting up to 40s @08:28:05.17 Intake @47.8psia, vacuum from 20.0 to 24.0psi @08:28:05.18 SS.reconfigure forSampling @08:28:05.21 SS.seek 25.00ml @08:28:05.91 Vacuum too high -- waiting up to 40s @08:28:06.38 Intake @47.5psia, vacuum from 20.0 to 24.0psi @08:28:06.39 SS.reconfigure forSampling @08:28:06.42 SS.seek 25.00ml @08:28:07.13 Vacuum too high -- waiting up to 40s @08:28:07.60 Intake @47.3psia, vacuum from 20.0 to 24.0psi @08:28:07.61 SS.reconfigure forSampling @08:28:07.64 SS.seek 25.00ml @08:28:08.24 Vacuum too high -- waiting up to 40s @08:28:08.73 Intake @47.2psia, vacuum from 20.0 to 24.0psi @08:28:08.74 SS.reconfigure forSampling @08:28:08.77 SS.seek 25.00ml @08:28:09.47 Vacuum too high -- waiting up to 40s @08:28:09.95 Intake @47.5psia, vacuum from 20.0 to 24.0psi @08:28:09.96 SS.reconfigure forSampling @08:28:09.99 SS.seek 25.00ml @08:28:10.75 Vacuum too high -- waiting up to 40s @08:28:11.23 Intake @48.5psia, vacuum from 20.0 to 24.0psi @08:28:11.24 SS.reconfigure forSampling @08:28:11.27 SS.seek 25.00ml @08:28:12.00 Vacuum too high -- waiting up to 40s @08:28:12.47 Intake @48.3psia, vacuum from 20.0 to 24.0psi @08:28:12.48 SS.reconfigure forSampling @08:28:12.51 SS.seek 25.00ml @08:28:13.22 Vacuum too high -- waiting up to 40s @08:28:13.68 Intake @47.8psia, vacuum from 20.0 to 24.0psi @08:28:13.70 SS.reconfigure forSampling @08:28:13.73 SS.seek 25.00ml @08:28:14.32 Vacuum too high -- waiting up to 40s @08:28:14.80 Intake @47.7psia, vacuum from 20.0 to 24.0psi @08:28:14.81 SS.reconfigure forSampling @08:28:14.84 SS.seek 25.00ml @08:28:15.29 SS.reconfigure SSconfig @08:28:15.32 SS.hold @08:28:15.48 Intake.select :close @08:28:15.63 ShallowSampler::Clogged in MAIN -- progress too slow after 170.2ml SSV.dial :exhaust @08:28:16.42 Exhaust.select :open @08:28:16.53 SS.reconfigure SSexhale @08:28:16.57 SS.seek 0.00ml @08:28:24.01 Exhaust.select :close @08:28:24.11 Sampled 170.2ml @08:28:24.12 collectSample: Complete Evacuating Puck (bubblePt=55.0psi, delay=15.0s) @08:28:24.13 Intake.select :close @08:28:24.19 CSV.dial :waste @08:28:24.22 CS.seek 0.000ml @08:28:24.32 SSV.dial CBV,avoiding => exhaust @08:28:24.34
CBV.dial SSV @08:28:24.37 CSV.dial :air @08:28:25.75 CS.seek 10.000ml @08:28:26.34
CTV.dial :air @08:28:27.10 Sleepy.power :sampler,:OFF @08:28:27.13
delay 15 seconds @08:28:42.14 SSV.dial :spit,avoiding => exhaust @08:28:43.46 CTV.dial CSV,avoiding => intake @08:28:43.48
SS.seek 0.00ml @08:28:43.58 SSV.dial CBV,avoiding => exhaust @08:28:50.48 CBV.dial between CSV and waste2b @08:28:51.42 CTV.dial CSV,avoiding => air @08:28:51.44
CSV.dial CTV @08:28:51.89 CS.reconfigure CSpressurize @08:28:51.92 CS.seek 0.000ml @08:29:16.01 BubblePoint: Intake @70.2psia, Outlet @14.7psia @08:29:16.02 SS.reconfigure SSconfig @08:29:16.05 SS.seek 10.00ml @08:29:21.96 delay 15 seconds @08:29:37.06 Evacuated: Intake @69.4psia, Outlet @-0.1psia SSV.dial :spit,avoiding => exhaust @08:29:38.39 SS.reconfigure SSexhale @08:29:38.42 SS.seek 0.00ml @08:29:43.79 SS.reconfigure SSfast @08:29:43.83 SS.seek 5.00ml @08:29:46.33 SSV.dial CBV,avoiding => exhaust @08:29:46.35
CBV.dial SSV @08:29:47.69 CTV.dial :air,avoiding => intake @08:29:49.00 CSV.dial CTV @08:29:49.01
SS.reconfigure SSconfig @08:29:49.05 SS.seek 10.00ml @08:29:50.01 CSV.dial :air @08:29:50.53 CS.reconfigure CSfast @08:29:50.56 CS.seek 0.000ml @08:29:52.21
SSV.dial :spit,avoiding => exhaust @08:29:52.99 CS.coast @08:29:53.53
SS.reconfigure SSexhale @08:29:53.56 SS.seek 0.00ml @08:29:58.55 SS.reconfigure SSfast @08:29:58.59 SS.seek 5.00ml @08:30:01.55 SSV.dial CBV,avoiding => exhaust @08:30:01.57
CBV.dial SSV @08:30:03.87 SSV.dial :spit,avoiding => exhaust @08:30:05.19 SS.reconfigure SSconfig @08:30:05.22 SS.seek 0.00ml @08:30:08.21 SS.coast @08:30:08.24 Evacuating Puck (bubblePt=55.0psi, delay=15.0s) Intake.select :close @08:30:08.30 CSV.dial :waste @08:30:08.40 SSV.dial CBV,avoiding => exhaust @08:30:08.42
CBV.dial SSV @08:30:09.05 CS.seek 0.000ml @08:30:09.33 CSV.dial :air @08:30:09.74
CTV.dial :air @08:30:09.75 delay 15 seconds @08:30:10.12 CS.seek 10.000ml @08:30:24.75
SSV.dial :spit,avoiding => exhaust @08:30:26.08 CTV.dial CSV,avoiding => intake @08:30:26.10
SS.reconfigure SSexhale @08:30:26.17 SS.seek 0.00ml @08:30:26.52 SSV.dial CBV,avoiding => exhaust @08:30:34.20 CBV.dial between CSV and waste2b @08:30:35.13 CSV.dial between air and CTV @08:30:35.84 CS.seek 7.452ml @08:30:42.51 CTV.dial CSV,avoiding => air @08:30:42.52
CSV.dial CTV @08:30:43.02 CS.reconfigure CSpressurize @08:30:43.05 CS.seek 0.000ml @08:31:02.50 CSV.dial :air @08:31:02.93 CS.reconfigure CSfast @08:31:02.96 CS.seek 10.000ml @08:31:26.72 CSV.dial between air and CTV @08:31:27.10 CS.seek 2.366ml @08:31:45.41 CTV.dial CSV,avoiding => air @08:31:45.42
CSV.dial CTV @08:31:45.71 CS.reconfigure CSpressurize @08:31:45.74 CS.seek 0.000ml @08:31:48.80 BubblePoint: Intake @73.1psia, Outlet @17.8psia SS.reconfigure SSconfig @08:31:48.84 SS.seek 10.00ml @08:31:54.50 delay 15 seconds @08:32:09.60 Evacuated: Intake @72.3psia, Outlet @-0.1psia SSV.dial :spit,avoiding => exhaust @08:32:10.93 SS.reconfigure SSexhale @08:32:10.96 SS.seek 0.00ml @08:32:15.87 SS.reconfigure SSfast @08:32:15.90 SS.seek 5.00ml @08:32:18.63 SSV.dial CBV,avoiding => exhaust @08:32:18.65
CBV.dial SSV @08:32:19.97 CTV.dial :air,avoiding => intake @08:32:21.26 CSV.dial CTV @08:32:21.28
SS.reconfigure SSconfig @08:32:21.32 SS.seek 10.00ml @08:32:22.28 CSV.dial :air @08:32:22.78 CS.reconfigure CSfast @08:32:22.81 CS.seek 0.000ml @08:32:25.07
SSV.dial :spit,avoiding => exhaust @08:32:26.40 SS.reconfigure SSexhale @08:32:26.43 SS.seek 0.00ml @08:32:26.77 CS.coast @08:32:31.82
SS.reconfigure SSfast @08:32:31.86 SS.seek 5.00ml @08:32:34.49 SSV.dial CBV,avoiding => exhaust @08:32:34.51
CBV.dial SSV @08:32:36.82 SSV.dial :spit,avoiding => exhaust @08:32:38.14 SS.reconfigure SSconfig @08:32:38.17 SS.seek 0.00ml @08:32:41.32 SS.coast @08:32:41.35 Lysing: Adding 1.5ml lysis CBV.dial between CSV and waste1,avoiding => DBV @08:32:42.81 CTV.dial CSV @08:32:44.07 CSV.dial CSR @08:32:44.85 CSR.select :lysis @08:32:44.95 CS.reconfigure CSconfig @08:32:44.99 CS.seek 1.000ml @08:32:48.30 delay 5 seconds @08:32:53.32 CSR.select :air @08:32:53.46 CS.seek 6.000ml @08:33:06.90 delay 2 seconds @08:33:08.92 CSV.dial :waste1 @08:33:10.17 CS.reconfigure CSfast @08:33:10.20 CS.seek empty @08:33:24.65 CSV.dial CSR @08:33:25.91 CSR.select :lysis @08:33:26.01 CS.reconfigure CSconfig @08:33:26.05 CS.seek 1.500ml @08:33:30.80 delay 5 seconds @08:33:35.82 CSR.select :air @08:33:35.96 CS.seek 4.501ml @08:33:44.46 delay 2 seconds @08:33:46.48 CSV.dial CTV @08:33:46.98 CS.seek 1.275ml @08:33:55.91 Heating sh1 puck from tube 2 in Collection Clamp to 85.0C for 5:00 @08:33:55.92 CH.seek 85.0C,8:00 @08:36:53.51 CH.hold 5:00,85.0C @08:41:53.62 Collection Puck Heater at 85.0C after lysing @08:41:53.63 CS.seek 0.000ml @08:41:57.70 CTV.dial between spare and air,avoiding => intake @08:41:58.69 CSV.dial :air @08:41:59.13 CS.seek 5.000ml @08:42:12.62 CSV.dial CTV @08:42:13.12 delay 2 seconds @08:42:15.13 CSV.dial :air @08:42:15.62 delay 2 seconds @08:42:17.63 CSV.dial CTV @08:42:18.12 delay 2 seconds @08:42:20.13 CSV.dial :air @08:42:20.62 delay 2 seconds @08:42:22.63 CS.seek 0.000ml @08:42:36.25 Recovering Lysate into Collection syringe @08:42:36.26 CSV.dial CBV @08:42:37.25 CBV.dial CSV @08:42:37.61 CS.seek 5.000ml @08:42:51.12 delay 5 seconds @08:42:56.14 CTV.dial :air @08:42:56.61 CS.seek 7.000ml @08:43:02.43 delay 5 seconds @08:43:07.44 CSV.dial :air2 @08:43:07.86 CS.seek 10.000ml @08:43:16.25 CSV.dial between air and CTV,avoiding => CBV @08:43:17.38 Lysing Complete @08:43:17.43 Storing sh1 puck in Collection Clamp to tube 1 @08:43:17.55 SC.reconfigure SCtight @08:43:17.58 SC.seek 1 @08:43:19.69 SC.reconfigure SCloose @08:43:19.86 Elbow.reconfigure TightElbow @08:43:19.89 Elbow.seek 1 @08:43:21.52 Forearm.reconfigure TightForearm @08:43:21.55 Forearm.seek 1 + 800 counts @08:43:22.59 SE.reconfigure SEpick @08:43:22.64 SE.seek beamBreak @08:43:24.74 SE.configure SEconfig @08:43:24.86 Elbow.reconfigure LimpElbow @08:43:24.89 Forearm.reconfigure LooseForearm @08:43:24.93 Forearm.seek 1 @08:43:25.13 Elbow.hold @08:43:25.18 Elbow.reconfigure TightElbow @08:43:25.26 SE.seek 8.05 Pucks below Top Plate @08:43:25.67 Forearm.coast @08:43:25.70 Forearm.seek 1 + 343 counts @08:43:25.94 Forearm.reconfigure TightForearm @08:43:25.97 Forearm.seek 1 + 800 counts @08:43:26.21 SE.seek 9.05 Pucks below Top Plate @08:43:26.83 Forearm.reconfigure LooseForearm @08:43:26.86 Forearm.seek retract @08:43:27.78 Elbow.seek CC @08:43:29.30 Elbow.reconfigure LooseElbow @08:43:29.39 Forearm.seek CC @08:43:30.35 CC.seek sealed @08:43:31.28 Hand.grip @08:43:32.02 CC.seek open @08:43:35.35 Forearm.seek retract @08:43:36.32 Elbow.reconfigure TightElbow @08:43:36.36 Elbow.seek 1 @08:43:37.96 Elbow.reconfigure LimpElbow @08:43:38.01 Forearm.seek 1 @08:43:39.01 Elbow.hold @08:43:39.07 Elbow.reconfigure TightElbow @08:43:39.11 Hand.release @08:43:39.97 Forearm.coast @08:43:40.00 Forearm.seek 1 + 329 counts @08:43:40.26 Forearm.reconfigure TightForearm @08:43:40.29 Forearm.seek 1 + 800 counts @08:43:40.62 SE.seek down @08:43:41.72
Forearm.reconfigure LooseForearm @08:43:41.76 Forearm.seek retract @08:43:42.42 SE.coast @08:43:42.66
SC.coast @08:43:42.69 Tranferring lysate to PRV @08:43:42.70 CSV.dial :air @08:43:42.99 CS.seek 10.000ml @08:43:43.06 CSV.dial between air and CTV @08:43:43.44 AIV.dial :bypass @08:43:43.50 Beginning samplePassC @08:43:43.51 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:43:43.66 Elbow.seek FlushPuck::Garage @08:43:45.40 Elbow.reconfigure LooseElbow @08:43:45.48 Forearm.seek garage @08:43:46.49 Hand.grip @08:43:47.36 Forearm.seek retract @08:43:48.38 Elbow.reconfigure TightElbow @08:43:48.42 Elbow.seek CC @08:43:51.25 Elbow.reconfigure LooseElbow @08:43:51.34 Forearm.reconfigure TightForearm @08:43:51.37 Forearm.seek CC @08:43:52.46 CC.seek closed @08:43:56.27 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.99 @08:43:56.32 Hand.release @08:43:57.06 Forearm.reconfigure LooseForearm @08:43:57.09 Forearm.seek retract @08:43:58.05 AWV.select :air @08:43:58.06 AIV.dial :bypass @08:43:58.07 PSV.dial PSR @08:43:59.48 PSR.select :flush @08:43:59.58 PS.seek 1.500ml @08:44:15.69 delay 5 seconds @08:44:20.70 PSV.dial :waste1 @08:44:21.16 PS.seek 0.000ml @08:44:37.26 PSV.dial PSR @08:44:37.82 PSR.select :air @08:44:37.92 PS.seek 2.500ml @08:45:04.14 CBV.dial PRV @08:45:04.15 PRV.dial CBV @08:45:04.93
PSV.dial :mixloop @08:45:05.94 CTV.dial :air,avoiding => intake @08:45:05.95 PTV.dial :PRVmixing @08:45:06.03 PS.seek 0.000ml @08:45:32.35 PSV.dial :air @08:45:32.80 PS.seek 2.500ml @08:45:59.03 PSV.dial :mixloop @08:45:59.48 PS.seek 0.000ml @08:46:25.69 PSV.dial :air @08:46:26.14 PS.seek 2.500ml @08:46:52.31 PSV.dial :mixloop @08:46:52.76 PS.seek 0.000ml @08:47:19.00 Garaging FlushPuck in Collection Clamp @08:47:19.06 Elbow.reconfigure TightElbow @08:47:19.09 Elbow.seek CC @08:47:19.26 Elbow.reconfigure LooseElbow @08:47:19.95 Forearm.seek CC @08:47:20.90 CC.seek sealed @08:47:21.86 Hand.grip @08:47:22.63 CC.seek open @08:47:25.93 Forearm.seek retract @08:47:26.89 Elbow.reconfigure TightElbow @08:47:26.93 Elbow.seek garage @08:47:29.72 Elbow.reconfigure LooseElbow @08:47:29.80 Forearm.reconfigure TightForearm @08:47:29.83 Forearm.seek garage @08:47:31.00 Hand.release @08:47:31.80 Forearm.reconfigure LooseForearm @08:47:31.83 Forearm.seek retract @08:47:32.86 Connecting PS to CS to transfer sample @08:47:32.87 CBV.dial AIV @08:47:32.88 AIV.dial :bypass @08:47:32.89 PSV.dial :mixloop @08:47:32.90 PTV.dial :PRVmixing PRV.dial AIV @08:47:32.91 CTV.dial :air,avoiding => intake @08:47:32.92 Loading lyfil puck from tube 2 into Collection Clamp @08:47:33.10 SC.reconfigure SCtight @08:47:33.13 SC.seek 2 @08:47:35.37 SC.reconfigure SCloose @08:47:35.55 Elbow.reconfigure TightElbow @08:47:35.58 Elbow.seek 2 @08:47:37.39 Forearm.reconfigure TightForearm @08:47:37.42 Forearm.seek 2 + 800 counts @08:47:38.49 SE.reconfigure SEpick @08:47:38.54 SE.seek beamBreak @08:47:40.43 SE.configure SEconfig @08:47:40.55 Elbow.seek 2 @08:47:40.69 Forearm.seek 2 + 300 counts @08:47:40.98 Forearm.reconfigure LooseForearm @08:47:41.02 Hand.grip @08:47:41.78 SE.seek down @08:47:41.92
Forearm.seek retract @08:47:42.91 Elbow.seek CC @08:47:43.48 SE.coast @08:47:44.43
Elbow.reconfigure LooseElbow @08:47:44.51 Forearm.reconfigure TightForearm @08:47:44.54 Forearm.seek CC @08:47:45.62 CC.seek closed @08:47:49.39 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.98 @08:47:49.40 Hand.release @08:47:50.18 Forearm.reconfigure LooseForearm @08:47:50.21 Forearm.seek retract @08:47:51.19 SC.coast @08:47:51.22 CTV.dial CSV,avoiding => intake @08:47:52.51 CSV.dial CTV @08:47:52.80 CS.seek 5.000ml @08:48:06.24 delay 1:20 @08:49:26.25 CS.seek 2.500ml @08:49:33.43 delay 40 seconds @08:50:13.44 CS.seek 0.000ml @08:50:20.55 delay 1:00 @08:51:20.56 PSV.dial :air @08:51:21.01 PS.seek 2.500ml @08:51:47.22 PSV.dial :mixloop @08:51:47.66 delay 3 seconds @08:51:50.67 PSV.dial :air @08:51:51.11 delay 2 seconds @08:51:53.12 PSV.dial :mixloop @08:51:53.56 delay 3 seconds @08:51:56.57 PSV.dial :waste1 @08:51:57.84 PS.seek empty @08:52:24.12 CBV.dial :waste1 @08:52:24.58 delay 5 seconds @08:52:29.59 CBV.dial AIV @08:52:30.04 delay 10 seconds @08:52:40.05 CBV.dial :waste1 @08:52:40.51 delay 10 seconds @08:52:50.52 CBV.dial AIV @08:52:50.96 delay 10 seconds @08:53:00.97 CTV.dial CSV,avoiding => intake @08:53:00.98 CBV.dial between AIV and waste1 @08:53:01.31 CSV.dial :air @08:53:01.82 CS.seek 10.000ml @08:53:27.98 CSV.dial CTV @08:53:28.49 CS.seek 0.000ml @08:53:54.54 CBV.dial :waste1 @08:53:54.84 delay 10 seconds @08:54:04.85 CTV.dial :air,avoiding => intake @08:54:06.76 Storing lyfil puck in Collection Clamp to tube 1 @08:54:06.89 SC.reconfigure SCtight @08:54:06.92 SC.seek 1 @08:54:09.20 SC.reconfigure SCloose @08:54:09.38 Elbow.reconfigure TightElbow @08:54:09.41 Elbow.seek 1 @08:54:11.06 Forearm.reconfigure TightForearm @08:54:11.09 Forearm.seek 1 + 800 counts @08:54:12.07 SE.reconfigure SEpick @08:54:12.12 SE.seek beamBreak @08:54:14.19 SE.configure SEconfig @08:54:14.31 Elbow.reconfigure LimpElbow @08:54:14.34 Forearm.reconfigure LooseForearm @08:54:14.38 Forearm.seek 1 @08:54:14.61 Elbow.hold @08:54:14.66 Elbow.reconfigure TightElbow @08:54:14.74 SE.seek 9.05 Pucks below Top Plate @08:54:15.07 Forearm.coast @08:54:15.10 Forearm.seek 1 + 333 counts @08:54:15.34 Forearm.reconfigure TightForearm @08:54:15.37 Forearm.seek 1 + 800 counts @08:54:15.61 SE.seek 10.05 Pucks below Top Plate @08:54:16.19 Forearm.reconfigure LooseForearm @08:54:16.22 Forearm.seek retract @08:54:17.19 Elbow.seek CC @08:54:18.72 Elbow.reconfigure LooseElbow @08:54:18.81 Forearm.seek CC @08:54:19.80 CC.seek sealed @08:54:20.73 Hand.grip @08:54:21.50 CC.seek open @08:54:24.82 Forearm.seek retract @08:54:25.77 Elbow.reconfigure TightElbow @08:54:25.81 Elbow.seek 1 @08:54:27.41 Elbow.reconfigure LimpElbow @08:54:27.44 Forearm.seek 1 @08:54:28.47 Elbow.hold @08:54:28.52 Elbow.reconfigure TightElbow @08:54:28.56 Hand.release @08:54:29.33 Forearm.coast @08:54:29.36 Forearm.seek 1 + 324 counts @08:54:29.59 Forearm.reconfigure TightForearm @08:54:29.62 Forearm.seek 1 + 800 counts @08:54:29.90 SE.seek down @08:54:31.02
Forearm.reconfigure LooseForearm @08:54:31.05 Forearm.seek retract @08:54:31.67 SE.coast @08:54:31.96
SC.coast @08:54:31.98 PSV.dial :air @08:54:33.03 PS.seek 0.499ml @08:54:38.96 delay 3 seconds @08:54:41.97 PSV.dial :mixloop @08:54:42.40 PRV.dial :air @08:54:42.85 delay 3 seconds @08:54:45.86 PRV.dial CBV @08:54:46.31 CBV.dial AIV @08:54:46.77 PRV.dial between CBV and air @08:54:47.09 PRV.dial :air @08:54:47.42 PS.reconfigure PSslow @08:54:47.46 PS.seek 0.599ml @08:54:50.02 PRV.dial :waste2 @08:54:50.75 PS.reconfigure PSconfig @08:54:50.78 PS.seek empty @08:54:57.74 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:54:57.89 Elbow.seek FlushPuck::Garage @08:54:59.67 Elbow.reconfigure LooseElbow @08:54:59.76 Forearm.seek garage @08:55:00.78 Hand.grip @08:55:01.58 Forearm.seek retract @08:55:02.60 Elbow.reconfigure TightElbow @08:55:02.64 Elbow.seek CC @08:55:05.40 Elbow.reconfigure LooseElbow @08:55:06.09 Forearm.reconfigure TightForearm @08:55:06.12 Forearm.seek CC @08:55:07.18 CC.seek closed @08:55:10.97 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 2.00 @08:55:11.02 Hand.release @08:55:11.79 Forearm.reconfigure LooseForearm @08:55:11.82 Forearm.seek retract @08:55:12.80 Clearing PML for modified lysate @08:55:12.81 CTV.dial :air,avoiding => intake @08:55:12.82 PRV.dial :waste2 @08:55:12.83 PTV.dial :PRV,avoiding => puck @08:55:13.53 delay 2 seconds @08:55:15.53 PTV.dial :PRVmixing,avoiding => puck @08:55:16.26 delay 2 seconds @08:55:18.27 PSV.dial :PML @08:55:18.28 delay 2 seconds @08:55:20.28 PRV.dial :air @08:55:21.01 delay 2 seconds @08:55:23.07 PS.seek 1.499ml @08:55:39.15 delay 5 seconds @08:55:44.16 PS.seek 2.500ml @08:55:55.18 PSV.dial :air @08:55:55.64 PS.seek 0.000ml @08:56:21.90 mixing lysate with speDil @08:56:21.91 PSV.dial :PML @08:56:22.35 CBV.dial :PRV @08:56:22.36 PRV.dial :air @08:56:22.37 delay 2 seconds @08:56:24.38 PRV.dial :speDil @08:56:24.82 delay 2 seconds @08:56:26.88 PS.reconfigure PSslow1 @08:56:26.91 PS.seek 0.225ml @08:56:50.21 delay 5 seconds @08:56:55.22 PRV.dial CBV @08:56:55.94 delay 2 seconds @08:56:58.00 PS.seek 0.475ml @08:57:23.74 delay 5 seconds @08:57:28.75 PRV.dial :air @08:57:29.21 delay 2 seconds @08:57:31.22 PS.seek 0.925ml @08:58:17.45 delay 10 seconds @08:58:27.46 PRV.dial CBV @08:58:27.91 CTV.dial :air,avoiding => intake @08:58:27.92 PSV.dial :air @08:58:28.37 PS.reconfigure PSconfig @08:58:28.40 PS.seek 2.500ml @08:58:45.27 delay 3 seconds @08:58:48.28 PSV.dial PTV @08:58:49.00 PTV.dial :PRV,avoiding => puck @08:58:49.73 PS.seek 0.000ml @08:59:16.01 PSV.dial :air @08:59:16.74 PS.seek 2.500ml @08:59:43.01 delay 3 seconds @08:59:46.02 PSV.dial PTV @08:59:46.73 PTV.dial :PRV,avoiding => puck @08:59:46.75 PS.seek 0.000ml @09:00:12.98 Pushing modified lysate into AIV Injection Loop @09:00:12.99 CBV.dial AIV @09:00:13.00 AIV.dial :bypass @09:00:13.01 PRV.dial CBV @09:00:13.02 PSV.dial :air @09:00:13.74 PS.seek 2.500ml @09:00:40.01 delay 3 seconds @09:00:43.03 PSV.dial :PML @09:00:43.46 PTV.dial :PRVmixing,avoiding => puck @09:00:44.23 PS.reconfigure PSslow1 @09:00:44.26 PS.seek 1.850ml @09:01:50.73 delay 5 seconds @09:01:55.74 AIV.dial :sample @09:01:56.27 AWV.select :waste @09:01:56.43 PS.reconfigure PSslow2 @09:01:56.46 PS.seek 1.310ml @09:03:46.53 delay 15 seconds @09:04:01.54 AIV.dial :bypass @09:04:02.10 PS.reconfigure PSconfig @09:04:02.13 PS.seek 0.000ml @09:04:16.25 PSV.dial :air @09:04:16.70 PS.seek 2.500ml @09:04:42.98 PSV.dial :PML @09:04:43.43 PS.seek 0.000ml @09:05:09.70 AIV.dial :coilToMFB @09:05:09.71 AWV.select :air @09:05:09.85 Extracting Nucleic Acid from AIV @09:05:09.87
Beginning samplePassD @09:05:09.88 SPE.hold 2:46:40,55.0C @09:05:09.90 ASV.dial :SPEdelivery @09:05:10.03
AWV.select :air AIV.dial :bypass @09:05:10.04 PSV.dial PSR @09:05:10.36 delay 20 seconds @09:05:11.18
PSR.select :killMFB @09:05:11.28 PS.seek 1.500ml @09:05:27.35 delay 5 seconds @09:05:30.37 primeLysateINT @09:05:30.38 AWV.select :air @09:05:30.39 AIV.dial :coilToMFB ATV.dial :AIV @09:05:31.73 AS.reconfigure ASslow1 @09:05:31.77 AS.seek 0.239ml @09:05:32.36
PSV.dial :waste1 @09:05:32.82 PS.seek 0.000ml @09:05:48.88 PSV.dial PSR @09:05:49.34 PSR.select :air @09:05:49.44 PS.seek 2.500ml @09:06:15.76 PRV.dial CBV @09:06:15.78
CBV.dial PRV @09:06:15.79 PSV.dial :mixloop @09:06:16.82 CTV.dial :air,avoiding => intake @09:06:16.83 PTV.dial :PRVmixing @09:06:16.85 PS.seek 0.000ml @09:06:20.99 delay 10 seconds @09:06:31.00 ATV.dial :air2 @09:06:31.58 AS.seek 0.269ml @09:06:37.92 ATV.dial :waste2 @09:06:38.28 AS.reconfigure ASnorm @09:06:38.31 AS.seek empty @09:06:43.13
PSV.dial PSR @09:06:44.18 PSR.select :flush @09:06:44.28 PS.seek 1.500ml @09:06:52.47 Lysate primed! @09:06:52.48 AFV.select :holdingCoil @09:06:52.49 ATV.dial :air2 @09:06:52.88 AS.reconfigure ASslow1 @09:06:52.91 AS.seek 0.069ml @09:07:00.35
delay 5 seconds @09:07:05.36 PSV.dial :waste1 @09:07:05.81 PS.seek 0.000ml @09:07:07.52 delay 3 seconds @09:07:10.53 ATV.dial :AIV @09:07:11.11 AS.seek 0.469ml @09:07:21.88
PSV.dial PSR @09:07:22.33 PSR.select :air @09:07:22.43 PS.seek 2.500ml @09:07:48.66 PRV.dial CBV @09:07:48.68
CBV.dial PRV @09:07:48.69 PSV.dial :mixloop @09:07:49.71 CTV.dial :air,avoiding => intake @09:07:49.72 PTV.dial :PRVmixing @09:07:49.74 PS.seek 0.000ml @09:08:15.97 PSV.dial PSR @09:08:16.99 PSR.select :flush @09:08:17.09 PS.seek 1.500ml @09:08:33.01 delay 5 seconds @09:08:33.16
delay 5 seconds @09:08:38.06 Solid Phase Extraction Heater at 52.0C @09:08:38.07 ATV.dial ASV @09:08:38.17
PSV.dial :waste1 @09:08:38.40 ASV.dial :SPEdelivery @09:08:38.46 AS.seek 0.041ml @09:08:38.62
PS.seek 0.000ml @09:08:54.66 PSV.dial PSR @09:08:55.22 PSR.select :air @09:08:55.32 PS.seek 2.500ml @09:09:21.50 PRV.dial CBV @09:09:21.52
CBV.dial PRV @09:09:21.53 PSV.dial :mixloop @09:09:22.55 CTV.dial :air,avoiding => intake @09:09:22.56 PTV.dial :PRVmixing @09:09:22.58 PS.seek 0.000ml @09:09:48.81 PSV.dial PSR @09:09:49.81 PSR.select :flush @09:09:49.91 PS.seek 1.500ml @09:10:05.97 delay 5 seconds @09:10:06.03 delay 10 seconds @09:10:10.98
PSV.dial :waste1 @09:10:11.43 PS.seek 0.000ml @09:10:16.04 rinseLysateLineINT @09:10:16.05 AFV.select :carrierSolution @09:10:16.14 delay 2 seconds @09:10:18.15 AS.reconfigure ASconfig @09:10:18.18 AS.seek 1.000ml @09:10:27.53
PSV.dial PSR @09:10:28.09 PSR.select :air @09:10:28.19 PS.seek 2.500ml @09:10:54.47 PRV.dial CBV @09:10:54.49
CBV.dial PRV @09:10:54.50 PSV.dial :mixloop @09:10:55.51 CTV.dial :air,avoiding => intake @09:10:55.52 PTV.dial :PRVmixing @09:10:55.53 PS.reconfigure PSslow @09:10:55.57 PS.seek 1.200ml @09:11:14.37 delay 1 second @09:11:15.38 AFV.select :holdingCoil @09:11:15.47 delay 1 second @09:11:16.48 ATV.dial :AIV @09:11:16.82 AIV.dial :coilToMFB @09:11:16.83 AWV.select :waste @09:11:16.92 AS.reconfigure ASslow0 @09:11:16.96 AS.seek 0.000ml @09:11:22.43
PSV.dial :waste1 @09:11:23.74 PS.reconfigure PSconfig @09:11:23.77 PS.seek 0.000ml @09:11:36.84 PSV.dial :air @09:11:37.86 PS.seek 2.500ml @09:12:04.03 PSV.dial :mixloop @09:12:04.47 PS.reconfigure PSslow @09:12:04.50 PS.seek 0.000ml @09:12:55.84 PSV.dial :air @09:12:56.41 PS.reconfigure PSconfig @09:12:56.44 PS.seek 2.500ml @09:12:59.32 ATV.dial :air2 @09:12:59.88 AS.reconfigure ASslow1 @09:12:59.91 AS.seek 0.050ml @09:13:10.41 ATV.dial :AIV @09:13:10.99 AS.seek 0.010ml @09:13:19.94 ATV.dial :waste @09:13:21.23 AS.seek empty @09:13:22.65
PSV.dial :mixloop @09:13:23.08 PS.reconfigure PSslow @09:13:23.11 PS.seek 0.000ml @09:13:23.65 ethanol wash @09:13:23.66 ATV.dial :air2 @09:13:24.60 AS.reconfigure ASslow2 @09:13:24.63 AS.seek 0.009ml @09:13:35.19 delay 3 seconds @09:13:38.20 Pulling 0.4ml of Ethanol Wash @09:13:38.21 ATV.dial :ethanol @09:13:38.77 AS.reconfigure ASslow1 @09:13:38.80 AS.seek 0.409ml @09:14:14.49
Cleaning Collection Syringe @09:14:14.50 CSV.dial CSR @09:14:14.92 CSR.select :flush @09:14:15.02 CS.seek 4.000ml @09:14:25.99 delay 5 seconds @09:14:31.00 CSR.select :air @09:14:31.09 CS.seek 10.000ml @09:14:47.15 CSV.dial CTV @09:14:47.17
CTV.dial CSV @09:14:48.49 CBV.dial :waste1 @09:14:48.97 CS.seek 0.000ml @09:15:00.66 delay 3 seconds @09:15:03.67 ATV.dial :air1 @09:15:04.00 delay 3 seconds @09:15:07.06 AS.seek 0.434ml @09:15:12.41 delay 3 seconds @09:15:15.15
CSV.dial CSR @09:15:15.42 ATV.dial :ethanol @09:15:15.65
CSR.select :flush @09:15:15.75 CS.seek 4.000ml @09:15:15.86 AS.seek 0.419ml @09:15:19.19 delay 3 seconds @09:15:22.20 ATV.dial ASV @09:15:23.33 AS.seek 0.092ml @09:15:26.71
delay 5 seconds @09:15:31.72 CSR.select :air @09:15:31.81 CS.seek 10.000ml @09:15:47.77 CSV.dial CTV @09:15:47.79
CTV.dial CSV @09:15:48.30 CBV.dial :waste1 @09:15:48.32 CS.seek 0.000ml @09:16:14.48 Garaging FlushPuck in Collection Clamp @09:16:14.53 Elbow.reconfigure TightElbow @09:16:14.57 Elbow.seek CC @09:16:14.66 Elbow.reconfigure LooseElbow @09:16:14.74 Forearm.seek CC @09:16:15.72 CC.seek sealed @09:16:16.65 Hand.grip @09:16:17.33 CC.seek open @09:16:20.64 Forearm.seek retract @09:16:21.58 Elbow.reconfigure TightElbow @09:16:21.62 Elbow.seek garage @09:16:24.46 Elbow.reconfigure LooseElbow @09:16:24.54 Forearm.reconfigure TightForearm @09:16:24.57 Forearm.seek garage @09:16:25.74 Hand.release @09:16:26.49 Forearm.reconfigure LooseForearm @09:16:26.52 Forearm.seek retract @09:16:27.58 Whole Cell Archive sampling 1000ml @09:16:27.59 Loading wcr puck from tube 2 into Collection Clamp @09:16:27.76 SC.reconfigure SCtight @09:16:27.80 SC.seek 2 @09:16:30.06 SC.reconfigure SCloose @09:16:30.25 Elbow.reconfigure TightElbow @09:16:30.29 Elbow.seek 2 @09:16:30.31 delay 3 seconds @09:16:32.12 Forearm.reconfigure TightForearm @09:16:32.15 Forearm.seek 2 + 800 counts @09:16:33.27 SE.reconfigure SEpick @09:16:33.33 ATV.dial :waste2 @09:16:33.35 SE.seek beamBreak @09:16:33.87 AS.reconfigure ASconfig @09:16:33.90 AS.seek empty @09:16:35.35 SE.configure SEconfig @09:16:35.48 Elbow.seek 2 @09:16:35.62 Forearm.seek 2 + 300 counts @09:16:35.91 Forearm.reconfigure LooseForearm @09:16:35.95 Hand.grip @09:16:36.72 SE.seek down @09:16:37.44 Forearm.seek retract @09:16:38.45 Elbow.seek CC @09:16:38.56 SE.coast @09:16:39.61 ATV.dial :air2 @09:16:39.95 AS.reconfigure ASslow1 @09:16:40.01 AS.seek 1.000ml @09:16:40.07 Elbow.reconfigure LooseElbow @09:16:40.16 Forearm.reconfigure TightForearm @09:16:40.19 Forearm.seek CC @09:16:41.32 CC.seek closed @09:16:45.15 Collection Clamp onSpring/offSpring for 3/21 samples, on/off torque 1.96 @09:16:45.16 Hand.release @09:16:45.99 Forearm.reconfigure LooseForearm @09:16:46.02 Forearm.seek retract @09:16:47.02 SC.coast @09:16:47.05 collectSample 1000ml w/bubble point of 55psi @09:16:47.07 Priming 7ml Sleepy.power :sampler,:ON @09:16:47.08 Intake.select :close @09:16:47.09 Exhaust.select :close @09:16:47.10 SS.hold @09:16:47.25 Calibrating pressure sensors @09:16:47.26 CTV.dial :air @09:16:48.62 SSV.dial :spit,avoiding => exhaust @09:16:48.63 SS.reconfigure SSfast @09:16:48.66 SS.seek 5.00ml @09:16:51.35 SSV.dial CBV @09:16:51.37 CBV.dial SSV @09:16:52.78 delay 3.8 seconds @09:16:56.67 SSV.dial :spit @09:16:58.03 SS.seek 0.00ml @09:17:00.91 SSV.dial CBV @09:17:02.25 CTV.dial :intake @09:17:03.00 Waiting for external sampler to finish priming... @09:17:03.01 delayUntil "09:26:47.07" @09:20:04.41 delay 5 seconds @09:20:09.42 ATV.dial ASV @09:20:10.13 ASV.dial :SPEdelivery @09:20:10.15 AS.seek 0.020ml @09:23:30.31 delay 5 seconds @09:23:35.32 ATV.dial :air2 @09:23:36.09 AS.seek 0.520ml @09:25:18.34 delay 5 seconds @09:25:23.35 ATV.dial ASV @09:25:24.07 ASV.dial :wasteR @09:25:24.67 AS.seek 0.300ml @09:26:09.77 delay 20 seconds @09:26:29.78 AS.seek 0.070ml @09:26:47.08 SSV.dial CBV @09:26:47.10 CBV.dial SSV @09:26:47.11 Allowing 55s for 7.0ml of remaining 7.0ml @09:26:47.12 Intake.select :open @09:26:49.25 Intake @52.0psia, vacuum from 20.0 to 24.0psi @09:26:49.27 SS.reconfigure forSampling @09:26:49.30 SS.seek 7.00ml @09:26:55.12 Stroke completed -- waiting for pressure to equalize ... @09:26:55.76 Intake.select :close @09:26:55.89 SSV.dial :exhaust @09:26:56.67 Exhaust.select :open @09:26:56.77 SS.reconfigure SSexhale @09:26:56.81 SS.seek 0.00ml @09:27:00.09 Exhaust.select :close @09:27:00.20 Sampling 1000.0ml @09:27:00.21 Intake.select :close @09:27:00.22 Exhaust.select :close @09:27:00.23 SS.reconfigure SSconfig @09:27:00.26 SS.hold @09:27:00.41 CTV.dial :intake @09:27:00.42 SSV.dial CBV @09:27:00.44 CBV.dial SSV @09:27:01.24 Allowing 185s for 25.0ml of remaining 1000.0ml @09:27:01.25 Intake.select :open @09:27:03.38 Intake @51.0psia, vacuum from 20.0 to 24.0psi @09:27:03.40 SS.reconfigure forSampling @09:27:03.43 SS.seek 25.00ml @09:27:16.91 delay 20 seconds @09:27:18.87 Stroke completed -- waiting for pressure to equalize ... @09:27:19.51 Intake.select :close @09:27:19.64 SSV.dial :exhaust @09:27:20.42 Exhaust.select :open @09:27:20.52 SS.reconfigure SSexhale @09:27:20.56 SS.seek 0.00ml @09:27:29.68 Exhaust.select :close @09:27:29.78 SSV.dial CBV @09:27:29.80 CBV.dial SSV @09:27:30.59 Allowing 185s for 25.0ml of remaining 975.0ml @09:27:30.60 Intake.select :open @09:27:32.74 Intake @51.5psia, vacuum from 20.0 to 24.0psi @09:27:32.76 SS.reconfigure forSampling @09:27:32.79 SS.seek 25.00ml @09:27:36.92 ATV.dial :waste2 @09:27:37.10 Vacuum too high -- waiting up to 40s @09:27:37.91 AS.reconfigure ASconfig @09:27:37.95 AS.seek empty @09:27:38.04 Intake @51.9psia, vacuum from 20.0 to 24.0psi @09:27:38.05 SS.reconfigure forSampling @09:27:38.09 SS.seek 25.00ml @09:27:42.20 Vacuum too high -- waiting up to 40s @09:27:42.44 Elution @09:27:42.45 ATV.dial :air1 @09:27:42.54 Intake @46.7psia, vacuum from 20.0 to 24.0psi @09:27:42.56 SS.reconfigure forSampling @09:27:42.59 SS.seek 25.00ml @09:27:43.41 AS.reconfigure ASslow1 @09:27:43.44 AS.seek 0.049ml @09:27:47.17 Vacuum too high -- waiting up to 40s @09:27:47.43 Intake @45.9psia, vacuum from 20.0 to 24.0psi @09:27:47.44 SS.reconfigure forSampling @09:27:47.48 SS.seek 25.00ml @09:27:50.92 Vacuum too high -- waiting up to 40s @09:27:51.40 Intake @49.4psia, vacuum from 20.0 to 24.0psi @09:27:51.42 SS.reconfigure forSampling @09:27:51.45 SS.seek 25.00ml @09:27:53.96 delay 3 seconds @09:27:54.58 Vacuum too high -- waiting up to 40s @09:27:54.86 Intake @47.8psia, vacuum from 20.0 to 24.0psi @09:27:54.87 SS.reconfigure forSampling @09:27:54.90 SS.seek 25.00ml @09:27:56.97 ATV.dial :ElutionWater @09:27:57.55 AS.seek 0.109ml @09:27:58.65 Stroke completed -- waiting for pressure to equalize ... @09:27:59.29 Intake.select :close @09:27:59.43 SSV.dial :exhaust @09:28:00.21 Exhaust.select :open @09:28:00.31 SS.reconfigure SSexhale @09:28:00.35 SS.seek 0.00ml @09:28:09.13 Exhaust.select :close @09:28:09.23 SSV.dial CBV @09:28:09.25 CBV.dial SSV @09:28:10.07 Allowing 185s for 25.0ml of remaining 950.0ml @09:28:10.08 Intake.select :open @09:28:10.12 delay 3 seconds @09:28:12.22 Intake @50.5psia, vacuum from 20.0 to 24.0psi @09:28:12.24 SS.reconfigure forSampling @09:28:12.27 SS.seek 25.00ml @09:28:13.13 ATV.dial ASV,via => air2 @09:28:14.80 ASV.dial :SPEelution @09:28:14.89 Vacuum too high -- waiting up to 40s @09:28:15.16 Intake @48.5psia, vacuum from 20.0 to 24.0psi @09:28:15.18 SS.reconfigure forSampling @09:28:15.21 SS.seek 25.00ml @09:28:15.31 AFV.select :carrierSolution @09:28:15.46 AS.reconfigure ASnorm @09:28:15.49 AS.seek 0.759ml @09:28:22.01 Vacuum too high -- waiting up to 40s @09:28:22.29 Intake @49.2psia, vacuum from 20.0 to 24.0psi @09:28:22.30 SS.reconfigure forSampling @09:28:22.34 SS.seek 25.00ml @09:28:33.38 Vacuum too high -- waiting up to 40s @09:28:33.65 Intake @49.3psia, vacuum from 20.0 to 24.0psi @09:28:33.67 SS.reconfigure forSampling @09:28:33.70 SS.seek 25.00ml @09:28:48.99 delay 3 seconds @09:28:52.00 AFV.select :holdingCoil @09:28:52.14 AS.reconfigure ASslow1 @09:28:52.17 AS.seek 0.489ml @09:29:00.62 Stroke completed -- waiting for pressure to equalize ... @09:29:01.85 Intake.select :close @09:29:01.98 SSV.dial :exhaust @09:29:02.77 Exhaust.select :open @09:29:02.87 SS.reconfigure SSexhale @09:29:02.91 SS.seek 0.00ml @09:29:12.13 Exhaust.select :close @09:29:12.23 SSV.dial CBV @09:29:12.25 CBV.dial SSV @09:29:13.04 Allowing 185s for 25.0ml of remaining 925.0ml @09:29:13.05 Intake.select :open @09:29:15.19 Intake @50.6psia, vacuum from 20.0 to 24.0psi @09:29:15.21 SS.reconfigure forSampling @09:29:15.24 SS.seek 25.00ml @09:29:20.91 Vacuum too high -- waiting up to 40s @09:29:21.18 Intake @48.8psia, vacuum from 20.0 to 24.0psi @09:29:21.19 SS.reconfigure forSampling @09:29:21.23 SS.seek 25.00ml @09:29:47.48 delay 5 seconds @09:29:52.54 AS.reconfigure ASslow2 @09:29:52.57 AS.seek 0.454ml @09:30:28.70 delay 1:00 @09:31:01.63 Stroke completed -- waiting for pressure to equalize ... @09:31:03.43 Intake.select :close @09:31:03.57 SSV.dial :exhaust @09:31:04.38 Exhaust.select :open @09:31:04.48 SS.reconfigure SSexhale @09:31:04.52 SS.seek 0.00ml @09:31:13.72 Exhaust.select :close @09:31:13.82 SSV.dial CBV @09:31:14.41 CBV.dial SSV @09:31:15.24 Allowing 185s for 25.0ml of remaining 900.0ml @09:31:15.25 Intake.select :open @09:31:17.38 Intake @49.1psia, vacuum from 20.0 to 24.0psi @09:31:17.40 SS.reconfigure forSampling @09:31:17.43 SS.seek 25.00ml @09:31:28.71 pushing eluate to holding coil @09:31:28.77 AS.seek 0.294ml @09:32:06.47 Vacuum too high -- waiting up to 40s @09:32:06.96 SS.seek 25.00ml @09:32:13.00 Vacuum too high -- waiting up to 40s @09:32:13.48 Intake @48.0psia, vacuum from 20.0 to 24.0psi @09:32:13.50 SS.reconfigure forSampling @09:32:13.53 SS.seek 25.00ml @09:32:25.19 Vacuum too high -- waiting up to 40s @09:32:25.67 Intake @48.4psia, vacuum from 20.0 to 24.0psi @09:32:25.68 SS.reconfigure forSampling @09:32:25.72 SS.seek 25.00ml @09:32:29.28 Vacuum too high -- waiting up to 40s @09:32:29.76 Intake @48.0psia, vacuum from 20.0 to 24.0psi @09:32:29.78 SS.reconfigure forSampling @09:32:29.81 SS.seek 25.00ml @09:32:33.35 Vacuum too high -- waiting up to 40s @09:32:33.83 Intake @48.1psia, vacuum from 20.0 to 24.0psi @09:32:33.85 SS.reconfigure forSampling @09:32:33.88 SS.seek 25.00ml @09:32:37.87 Vacuum too high -- waiting up to 40s @09:32:38.35 Intake @48.3psia, vacuum from 20.0 to 24.0psi @09:32:38.36 SS.reconfigure forSampling @09:32:38.40 SS.seek 25.00ml @09:32:41.43 Vacuum too high -- waiting up to 40s @09:32:41.92 Intake @48.4psia, vacuum from 20.0 to 24.0psi @09:32:41.93 SS.reconfigure forSampling @09:32:41.97 SS.seek 25.00ml @09:32:44.56 Vacuum too high -- waiting up to 40s @09:32:45.05 Intake @48.3psia, vacuum from 20.0 to 24.0psi @09:32:45.07 SS.reconfigure forSampling @09:32:45.10 SS.seek 25.00ml @09:32:48.00 Vacuum too high -- waiting up to 40s @09:32:48.48 Intake @48.5psia, vacuum from 20.0 to 24.0psi @09:32:48.49 SS.reconfigure forSampling @09:32:48.53 SS.seek 25.00ml @09:32:51.16 Vacuum too high -- waiting up to 40s @09:32:51.65 Intake @48.7psia, vacuum from 20.0 to 24.0psi @09:32:51.66 SS.reconfigure forSampling @09:32:51.70 SS.seek 25.00ml @09:32:54.13 Vacuum too high -- waiting up to 40s @09:32:54.62 Intake @48.5psia, vacuum from 20.0 to 24.0psi @09:32:54.63 SS.reconfigure forSampling @09:32:54.67 SS.seek 25.00ml @09:32:57.25 Vacuum too high -- waiting up to 40s @09:32:57.75 Intake @49.1psia, vacuum from 20.0 to 24.0psi @09:32:57.76 SS.reconfigure forSampling @09:32:57.79 SS.seek 25.00ml @09:32:59.94 Vacuum too high -- waiting up to 40s @09:33:00.42 Intake @48.6psia, vacuum from 20.0 to 24.0psi @09:33:00.44 SS.reconfigure forSampling @09:33:00.47 SS.seek 25.00ml @09:33:02.44 Vacuum too high -- waiting up to 40s @09:33:02.93 Intake @48.3psia, vacuum from 20.0 to 24.0psi @09:33:02.95 SS.reconfigure forSampling @09:33:02.98 SS.seek 25.00ml @09:33:05.22 Vacuum too high -- waiting up to 40s @09:33:05.71 Intake @48.0psia, vacuum from 20.0 to 24.0psi @09:33:05.73 SS.reconfigure forSampling @09:33:05.76 SS.seek 25.00ml @09:33:07.72 Vacuum too high -- waiting up to 40s @09:33:08.21 Intake @47.8psia, vacuum from 20.0 to 24.0psi @09:33:08.22 SS.reconfigure forSampling @09:33:08.26 SS.seek 25.00ml @09:33:10.87 Vacuum too high -- waiting up to 40s @09:33:12.19 Intake @48.8psia, vacuum from 20.0 to 24.0psi @09:33:12.20 SS.reconfigure forSampling @09:33:12.24 SS.seek 25.00ml @09:33:15.09 Vacuum too high -- waiting up to 40s @09:33:15.58 Intake @48.4psia, vacuum from 20.0 to 24.0psi @09:33:15.60 SS.reconfigure forSampling @09:33:15.63 SS.seek 25.00ml @09:33:17.56 Vacuum too high -- waiting up to 40s @09:33:18.05 Intake @48.2psia, vacuum from 20.0 to 24.0psi @09:33:18.07 SS.reconfigure forSampling @09:33:18.10 SS.seek 25.00ml @09:33:20.22 Vacuum too high -- waiting up to 40s @09:33:20.71 Intake @48.0psia, vacuum from 20.0 to 24.0psi @09:33:20.73 SS.reconfigure forSampling @09:33:20.76 SS.seek 25.00ml @09:33:21.68 Stroke completed -- waiting for pressure to equalize ... @09:33:24.68 Intake.select :close @09:33:24.82 SSV.dial :exhaust @09:33:25.63 Exhaust.select :open @09:33:25.73 SS.reconfigure SSexhale @09:33:25.77 SS.seek 0.00ml @09:33:34.93 Exhaust.select :close @09:33:35.03 SSV.dial CBV @09:33:35.05 CBV.dial SSV @09:33:35.85 Allowing 185s for 25.0ml of remaining 875.0ml @09:33:35.86 Intake.select :open @09:33:38.00 Intake @48.7psia, vacuum from 20.0 to 24.0psi @09:33:38.02 SS.reconfigure forSampling @09:33:38.05 SS.seek 25.00ml @09:33:41.31 Vacuum too high -- waiting up to 40s @09:33:41.81 Intake @48.2psia, vacuum from 20.0 to 24.0psi @09:33:41.83 SS.reconfigure forSampling @09:33:41.86 SS.seek 25.00ml @09:33:43.94 Vacuum too high -- waiting up to 40s @09:33:44.43 Intake @48.8psia, vacuum from 20.0 to 24.0psi @09:33:44.45 SS.reconfigure forSampling @09:33:44.48 SS.seek 25.00ml @09:33:46.34 Vacuum too high -- waiting up to 40s @09:33:46.83 Intake @48.4psia, vacuum from 20.0 to 24.0psi @09:33:46.84 SS.reconfigure forSampling @09:33:46.88 SS.seek 25.00ml @09:33:48.69 Vacuum too high -- waiting up to 40s @09:33:49.18 Intake @48.3psia, vacuum from 20.0 to 24.0psi @09:33:49.20 SS.reconfigure forSampling @09:33:49.23 SS.seek 25.00ml @09:33:51.03 Vacuum too high -- waiting up to 40s @09:33:51.52 Intake @48.1psia, vacuum from 20.0 to 24.0psi @09:33:51.54 SS.reconfigure forSampling @09:33:51.57 SS.seek 25.00ml @09:33:53.31 Vacuum too high -- waiting up to 40s @09:33:53.81 Intake @48.0psia, vacuum from 20.0 to 24.0psi @09:33:53.82 SS.reconfigure forSampling @09:33:53.86 SS.seek 25.00ml @09:33:55.53 Vacuum too high -- waiting up to 40s @09:33:56.02 Intake @47.8psia, vacuum from 20.0 to 24.0psi @09:33:56.04 SS.reconfigure forSampling @09:33:56.07 SS.seek 25.00ml @09:33:58.03 Vacuum too high -- waiting up to 40s @09:33:58.73 Intake @49.0psia, vacuum from 20.0 to 24.0psi @09:33:58.75 SS.reconfigure forSampling @09:33:58.78 SS.seek 25.00ml @09:34:00.53 Vacuum too high -- waiting up to 40s @09:34:01.02 Intake @49.0psia, vacuum from 20.0 to 24.0psi @09:34:01.03 SS.reconfigure forSampling @09:34:01.07 SS.seek 25.00ml @09:34:02.53 Vacuum too high -- waiting up to 40s @09:34:03.02 Intake @48.0psia, vacuum from 20.0 to 24.0psi @09:34:03.04 SS.reconfigure forSampling @09:34:03.07 SS.seek 25.00ml @09:34:04.62 Vacuum too high -- waiting up to 40s @09:34:05.12 SS.seek 25.00ml @09:34:08.15 Vacuum too high -- waiting up to 40s @09:34:08.65 Intake @48.1psia, vacuum from 20.0 to 24.0psi @09:34:08.66 SS.reconfigure forSampling @09:34:08.69 SS.seek 25.00ml @09:34:10.18 Vacuum too high -- waiting up to 40s @09:34:10.67 Intake @47.8psia, vacuum from 20.0 to 24.0psi @09:34:10.69 SS.reconfigure forSampling @09:34:10.72 SS.seek 25.00ml @09:34:12.18 delay 30 seconds @09:34:12.28 Vacuum too high -- waiting up to 40s @09:34:12.77 Intake @48.3psia, vacuum from 20.0 to 24.0psi @09:34:12.79 SS.reconfigure forSampling @09:34:12.82 SS.seek 25.00ml @09:34:14.31 Vacuum too high -- waiting up to 40s @09:34:14.80 Intake @48.6psia, vacuum from 20.0 to 24.0psi @09:34:14.82 SS.reconfigure forSampling @09:34:14.85 SS.seek 25.00ml @09:34:16.21 Vacuum too high -- waiting up to 40s @09:34:16.70 Intake @48.1psia, vacuum from 20.0 to 24.0psi @09:34:16.71 SS.reconfigure forSampling @09:34:16.75 SS.seek 25.00ml @09:34:18.25 Vacuum too high -- waiting up to 40s @09:34:18.73 Intake @48.4psia, vacuum from 20.0 to 24.0psi @09:34:18.75 SS.reconfigure forSampling @09:34:18.78 SS.seek 25.00ml @09:34:20.15 Vacuum too high -- waiting up to 40s @09:34:20.64 Intake @48.3psia, vacuum from 20.0 to 24.0psi @09:34:20.65 SS.reconfigure forSampling @09:34:20.69 SS.seek 25.00ml @09:34:22.09 Vacuum too high -- waiting up to 40s @09:34:22.58 Intake @48.2psia, vacuum from 20.0 to 24.0psi @09:34:22.60 SS.reconfigure forSampling @09:34:22.63 SS.seek 25.00ml @09:34:24.06 Vacuum too high -- waiting up to 40s @09:34:24.55 Intake @48.8psia, vacuum from 20.0 to 24.0psi @09:34:24.57 SS.reconfigure forSampling @09:34:24.60 SS.seek 25.00ml @09:34:25.87 Vacuum too high -- waiting up to 40s @09:34:26.36 Intake @48.4psia, vacuum from 20.0 to 24.0psi @09:34:26.37 SS.reconfigure forSampling @09:34:26.41 SS.seek 25.00ml @09:34:27.78 Vacuum too high -- waiting up to 40s @09:34:28.27 Intake @48.3psia, vacuum from 20.0 to 24.0psi @09:34:28.29 SS.reconfigure forSampling @09:34:28.32 SS.seek 25.00ml @09:34:29.71 Vacuum too high -- waiting up to 40s @09:34:30.42 Intake @49.0psia, vacuum from 20.0 to 24.0psi @09:34:30.44 SS.reconfigure forSampling @09:34:30.47 SS.seek 25.00ml @09:34:32.03 Vacuum too high -- waiting up to 40s @09:34:32.52 Intake @48.6psia, vacuum from 20.0 to 24.0psi @09:34:32.54 SS.reconfigure forSampling @09:34:32.57 SS.seek 25.00ml @09:34:33.87 Vacuum too high -- waiting up to 40s @09:34:34.36 Intake @48.3psia, vacuum from 20.0 to 24.0psi @09:34:34.38 SS.reconfigure forSampling @09:34:34.41 SS.seek 25.00ml @09:34:35.71 Vacuum too high -- waiting up to 40s @09:34:36.20 Intake @48.2psia, vacuum from 20.0 to 24.0psi @09:34:36.22 SS.reconfigure forSampling @09:34:36.25 SS.seek 25.00ml @09:34:37.56 Vacuum too high -- waiting up to 40s @09:34:38.05 Intake @48.6psia, vacuum from 20.0 to 24.0psi @09:34:38.07 SS.reconfigure forSampling @09:34:38.10 SS.seek 25.00ml @09:34:39.37 Vacuum too high -- waiting up to 40s @09:34:39.86 Intake @48.9psia, vacuum from 20.0 to 24.0psi @09:34:39.88 SS.reconfigure forSampling @09:34:39.91 SS.seek 25.00ml @09:34:41.12 Vacuum too high -- waiting up to 40s @09:34:41.98 Intake @48.1psia, vacuum from 20.0 to 24.0psi @09:34:42.00 SS.reconfigure forSampling @09:34:42.03 SS.seek 25.00ml @09:34:42.19 ATV.dial :waste,via => air3 @09:34:43.73 AS.reconfigure ASconfig @09:34:43.78 AS.seek empty @09:34:43.80 Vacuum too high -- waiting up to 40s @09:34:44.31 Intake @48.1psia, vacuum from 20.0 to 24.0psi @09:34:44.33 SS.reconfigure forSampling @09:34:44.36 SS.seek 25.00ml @09:34:45.71 Vacuum too high -- waiting up to 40s @09:34:46.21 Intake @48.6psia, vacuum from 20.0 to 24.0psi @09:34:46.23 SS.reconfigure forSampling @09:34:46.26 SS.seek 25.00ml @09:34:47.46 Vacuum too high -- waiting up to 40s @09:34:47.95 Intake @48.2psia, vacuum from 20.0 to 24.0psi @09:34:47.97 SS.reconfigure forSampling @09:34:48.00 SS.seek 25.00ml @09:34:49.28 Vacuum too high -- waiting up to 40s @09:34:49.77 Intake @47.7psia, vacuum from 20.0 to 24.0psi @09:34:49.79 SS.reconfigure forSampling @09:34:49.82 SS.seek 25.00ml @09:34:51.24 Vacuum too high -- waiting up to 40s @09:34:51.95 Intake @48.1psia, vacuum from 20.0 to 24.0psi @09:34:51.97 SS.reconfigure forSampling @09:34:52.00 SS.seek 25.00ml @09:34:53.59 Vacuum too high -- waiting up to 40s @09:34:54.29 Intake @49.0psia, vacuum from 20.0 to 24.0psi @09:34:54.31 SS.reconfigure forSampling @09:34:54.34 SS.seek 25.00ml @09:34:55.74 Vacuum too high -- waiting up to 40s @09:34:56.22 Intake @49.0psia, vacuum from 20.0 to 24.0psi @09:34:56.24 SS.reconfigure forSampling @09:34:56.27 SS.seek 25.00ml @09:34:57.43 Vacuum too high -- waiting up to 40s @09:34:57.92 Intake @48.6psia, vacuum from 20.0 to 24.0psi @09:34:57.93 SS.reconfigure forSampling @09:34:57.97 SS.seek 25.00ml @09:34:59.24 Vacuum too high -- waiting up to 40s @09:34:59.94 Intake @48.4psia, vacuum from 20.0 to 24.0psi @09:34:59.95 SS.reconfigure forSampling @09:34:59.99 SS.seek 25.00ml @09:35:01.24 Parking Bleach in LysateSource line and SPEcolumn @09:35:01.25 ATV.dial :air3 @09:35:01.34 Vacuum too high -- waiting up to 40s @09:35:01.88 Intake @48.1psia, vacuum from 20.0 to 24.0psi @09:35:01.90 SS.reconfigure forSampling @09:35:01.94 AS.reconfigure ASslow1 @09:35:01.97 SS.seek 25.00ml @09:35:02.03 AS.seek 0.029ml @09:35:03.28 Vacuum too high -- waiting up to 40s @09:35:03.78 Intake @48.0psia, vacuum from 20.0 to 24.0psi @09:35:03.79 SS.reconfigure forSampling @09:35:03.82 SS.seek 25.00ml @09:35:05.09 Vacuum too high -- waiting up to 40s @09:35:05.79 Intake @48.2psia, vacuum from 20.0 to 24.0psi @09:35:05.81 SS.reconfigure forSampling @09:35:05.84 SS.seek 25.00ml @09:35:07.40 Vacuum too high -- waiting up to 40s @09:35:08.11 Intake @48.5psia, vacuum from 20.0 to 24.0psi @09:35:08.12 SS.reconfigure forSampling @09:35:08.16 SS.seek 25.00ml @09:35:08.38 ATV.dial :ElutionWater @09:35:08.77 AS.seek 0.059ml @09:35:09.56 Vacuum too high -- waiting up to 40s @09:35:10.06 Intake @48.6psia, vacuum from 20.0 to 24.0psi @09:35:10.07 SS.reconfigure forSampling @09:35:10.10 SS.seek 25.00ml @09:35:11.21 Vacuum too high -- waiting up to 40s @09:35:12.33 Intake @48.1psia, vacuum from 20.0 to 24.0psi @09:35:12.34 SS.reconfigure forSampling @09:35:12.38 SS.seek 25.00ml @09:35:14.15 Vacuum too high -- waiting up to 40s @09:35:14.64 Intake @48.0psia, vacuum from 20.0 to 24.0psi @09:35:14.66 SS.reconfigure forSampling @09:35:14.69 SS.seek 25.00ml @09:35:15.07 ATV.dial :air3 @09:35:15.55 AS.seek 0.069ml @09:35:15.87 Vacuum too high -- waiting up to 40s @09:35:16.36 Intake @48.0psia, vacuum from 20.0 to 24.0psi @09:35:16.38 SS.reconfigure forSampling @09:35:16.41 SS.seek 25.00ml @09:35:17.56 Vacuum too high -- waiting up to 40s @09:35:17.86 delay 2 seconds @09:35:18.27 Intake @48.0psia, vacuum from 20.0 to 24.0psi @09:35:18.29 SS.reconfigure forSampling @09:35:18.32 SS.seek 25.00ml @09:35:19.71 Vacuum too high -- waiting up to 40s @09:35:19.92 ATV.dial :bleach @09:35:20.32 AS.seek 0.709ml @09:35:20.49 Intake @48.4psia, vacuum from 20.0 to 24.0psi @09:35:20.51 SS.reconfigure forSampling @09:35:20.54 SS.seek 25.00ml @09:35:22.18 Vacuum too high -- waiting up to 40s @09:35:22.90 Intake @49.0psia, vacuum from 20.0 to 24.0psi @09:35:22.92 SS.reconfigure forSampling @09:35:22.95 SS.seek 25.00ml @09:35:24.34 Vacuum too high -- waiting up to 40s @09:35:24.83 Intake @48.9psia, vacuum from 20.0 to 24.0psi @09:35:24.84 SS.reconfigure forSampling @09:35:24.88 SS.seek 25.00ml @09:35:25.93 Vacuum too high -- waiting up to 40s @09:35:26.63 Intake @48.7psia, vacuum from 20.0 to 24.0psi @09:35:26.65 SS.reconfigure forSampling @09:35:26.68 SS.seek 25.00ml @09:35:27.87 Vacuum too high -- waiting up to 40s @09:35:28.36 Intake @48.5psia, vacuum from 20.0 to 24.0psi @09:35:28.37 SS.reconfigure forSampling @09:35:28.41 SS.seek 25.00ml @09:35:29.55 Vacuum too high -- waiting up to 40s @09:35:30.04 Intake @48.4psia, vacuum from 20.0 to 24.0psi @09:35:30.06 SS.reconfigure forSampling @09:35:30.09 SS.seek 25.00ml @09:35:31.15 Vacuum too high -- waiting up to 40s @09:35:31.64 Intake @48.4psia, vacuum from 20.0 to 24.0psi @09:35:31.65 SS.reconfigure forSampling @09:35:31.69 SS.seek 25.00ml @09:35:32.68 Vacuum too high -- waiting up to 40s @09:35:33.40 Intake @48.5psia, vacuum from 20.0 to 24.0psi @09:35:33.42 SS.reconfigure forSampling @09:35:33.45 SS.seek 25.00ml @09:35:34.71 Vacuum too high -- waiting up to 40s @09:35:35.40 Intake @48.9psia, vacuum from 20.0 to 24.0psi @09:35:35.42 SS.reconfigure forSampling @09:35:35.45 SS.seek 25.00ml @09:35:36.74 Vacuum too high -- waiting up to 40s @09:35:37.43 Intake @49.5psia, vacuum from 20.0 to 24.0psi @09:35:37.45 SS.reconfigure forSampling @09:35:37.48 SS.seek 25.00ml @09:35:38.59 Vacuum too high -- waiting up to 40s @09:35:39.08 Intake @48.8psia, vacuum from 20.0 to 24.0psi @09:35:39.10 SS.reconfigure forSampling @09:35:39.13 SS.seek 25.00ml @09:35:40.21 Vacuum too high -- waiting up to 40s @09:35:40.91 Intake @48.7psia, vacuum from 20.0 to 24.0psi @09:35:41.57 SS.reconfigure forSampling @09:35:41.61 SS.seek 25.00ml @09:35:43.46 Vacuum too high -- waiting up to 40s @09:35:43.96 Intake @48.1psia, vacuum from 20.0 to 24.0psi @09:35:43.97 SS.reconfigure forSampling @09:35:44.01 SS.seek 25.00ml @09:35:45.09 Vacuum too high -- waiting up to 40s @09:35:45.80 Intake @48.3psia, vacuum from 20.0 to 24.0psi @09:35:45.82 SS.reconfigure forSampling @09:35:45.85 SS.seek 25.00ml @09:35:47.02 Vacuum too high -- waiting up to 40s @09:35:47.73 Intake @48.2psia, vacuum from 20.0 to 24.0psi @09:35:47.75 SS.reconfigure forSampling @09:35:47.78 SS.seek 25.00ml @09:35:49.09 Vacuum too high -- waiting up to 40s @09:35:49.79 Intake @48.1psia, vacuum from 20.0 to 24.0psi @09:35:49.81 SS.reconfigure forSampling @09:35:49.84 SS.seek 25.00ml @09:35:51.09 Vacuum too high -- waiting up to 40s @09:35:51.80 Intake @49.5psia, vacuum from 20.0 to 24.0psi @09:35:51.82 SS.reconfigure forSampling @09:35:51.85 SS.seek 25.00ml @09:35:52.80 Vacuum too high -- waiting up to 40s @09:35:53.29 Intake @49.0psia, vacuum from 20.0 to 24.0psi @09:35:53.31 SS.reconfigure forSampling @09:35:53.34 SS.seek 25.00ml @09:35:54.40 Vacuum too high -- waiting up to 40s @09:35:54.89 Intake @48.8psia, vacuum from 20.0 to 24.0psi @09:35:54.91 SS.reconfigure forSampling @09:35:54.94 SS.seek 25.00ml @09:35:56.02 Vacuum too high -- waiting up to 40s @09:35:56.80 Intake @48.4psia, vacuum from 20.0 to 24.0psi @09:35:56.81 SS.reconfigure forSampling @09:35:56.84 SS.seek 25.00ml @09:35:58.24 Vacuum too high -- waiting up to 40s @09:35:58.95 Intake @48.1psia, vacuum from 20.0 to 24.0psi @09:35:58.97 SS.reconfigure forSampling @09:35:59.00 SS.seek 25.00ml @09:36:00.21 Vacuum too high -- waiting up to 40s @09:36:00.92 Intake @48.1psia, vacuum from 20.0 to 24.0psi @09:36:00.94 SS.reconfigure forSampling @09:36:00.97 SS.seek 25.00ml @09:36:02.24 Vacuum too high -- waiting up to 40s @09:36:02.96 Intake @48.0psia, vacuum from 20.0 to 24.0psi @09:36:02.97 SS.reconfigure forSampling @09:36:03.00 SS.seek 25.00ml @09:36:04.21 Vacuum too high -- waiting up to 40s @09:36:04.92 Intake @47.9psia, vacuum from 20.0 to 24.0psi @09:36:04.93 SS.reconfigure forSampling @09:36:04.97 SS.seek 25.00ml @09:36:06.12 Vacuum too high -- waiting up to 40s @09:36:06.82 Intake @49.2psia, vacuum from 20.0 to 24.0psi @09:36:06.83 SS.reconfigure forSampling @09:36:06.87 SS.seek 25.00ml @09:36:07.99 Vacuum too high -- waiting up to 40s @09:36:08.71 Intake @48.8psia, vacuum from 20.0 to 24.0psi @09:36:08.73 SS.reconfigure forSampling @09:36:08.76 SS.seek 25.00ml @09:36:09.99 Vacuum too high -- waiting up to 40s @09:36:10.69 Intake @48.4psia, vacuum from 20.0 to 24.0psi @09:36:10.71 SS.reconfigure forSampling @09:36:10.74 SS.seek 25.00ml @09:36:11.87 Vacuum too high -- waiting up to 40s @09:36:12.75 Intake @48.1psia, vacuum from 20.0 to 24.0psi @09:36:12.77 SS.reconfigure forSampling @09:36:12.80 SS.seek 25.00ml @09:36:14.12 Vacuum too high -- waiting up to 40s @09:36:14.84 Intake @48.0psia, vacuum from 20.0 to 24.0psi @09:36:14.85 SS.reconfigure forSampling @09:36:14.88 SS.seek 25.00ml @09:36:16.08 Vacuum too high -- waiting up to 40s @09:36:16.80 SS.seek 25.00ml @09:36:17.80 Vacuum too high -- waiting up to 40s @09:36:18.51 Intake @48.2psia, vacuum from 20.0 to 24.0psi @09:36:18.53 SS.reconfigure forSampling @09:36:18.56 SS.seek 25.00ml @09:36:19.61 Vacuum too high -- waiting up to 40s @09:36:20.32 Intake @48.9psia, vacuum from 20.0 to 24.0psi @09:36:20.33 SS.reconfigure forSampling @09:36:20.36 SS.seek 25.00ml @09:36:21.55 Vacuum too high -- waiting up to 40s @09:36:22.26 Intake @48.9psia, vacuum from 20.0 to 24.0psi @09:36:22.28 SS.reconfigure forSampling @09:36:22.31 SS.seek 25.00ml @09:36:23.39 Vacuum too high -- waiting up to 40s @09:36:24.10 Intake @48.7psia, vacuum from 20.0 to 24.0psi @09:36:24.12 SS.reconfigure forSampling @09:36:24.15 SS.seek 25.00ml @09:36:25.36 Vacuum too high -- waiting up to 40s @09:36:26.07 Intake @48.4psia, vacuum from 20.0 to 24.0psi @09:36:26.08 SS.reconfigure forSampling @09:36:26.12 SS.seek 25.00ml @09:36:27.27 Vacuum too high -- waiting up to 40s @09:36:27.97 Intake @48.4psia, vacuum from 20.0 to 24.0psi @09:36:27.98 SS.reconfigure forSampling @09:36:28.02 SS.seek 25.00ml @09:36:29.18 Vacuum too high -- waiting up to 40s @09:36:29.89 Intake @48.2psia, vacuum from 20.0 to 24.0psi @09:36:29.91 SS.reconfigure forSampling @09:36:29.94 SS.seek 25.00ml @09:36:31.08 Vacuum too high -- waiting up to 40s @09:36:31.79 Intake @47.9psia, vacuum from 20.0 to 24.0psi @09:36:31.81 SS.reconfigure forSampling @09:36:31.84 SS.seek 25.00ml @09:36:33.02 Vacuum too high -- waiting up to 40s @09:36:33.94 Intake @51.8psia, vacuum from 20.0 to 24.0psi @09:36:33.96 SS.reconfigure forSampling @09:36:33.99 SS.seek 25.00ml @09:36:34.93 Vacuum too high -- waiting up to 40s @09:36:35.20 Intake @49.2psia, vacuum from 20.0 to 24.0psi @09:36:35.22 SS.reconfigure forSampling @09:36:35.25 SS.seek 25.00ml @09:36:36.27 Vacuum too high -- waiting up to 40s @09:36:36.98 Intake @48.7psia, vacuum from 20.0 to 24.0psi @09:36:36.99 SS.reconfigure forSampling @09:36:37.03 SS.seek 25.00ml @09:36:38.30 Vacuum too high -- waiting up to 40s @09:36:39.01 Intake @49.1psia, vacuum from 20.0 to 24.0psi @09:36:39.02 SS.reconfigure forSampling @09:36:39.06 SS.seek 25.00ml @09:36:39.20 Stroke completed -- waiting for pressure to equalize ... @09:36:40.86 SS.reconfigure SSconfig @09:36:41.55 SS.hold @09:36:41.64 Intake.select :close @09:36:41.79 ShallowSampler::Clogged in WCR -- progress too slow after 150.0ml @09:36:41.80 SSV.dial :exhaust @09:36:42.59 Exhaust.select :open @09:36:42.69 SS.reconfigure SSexhale @09:36:42.73 SS.seek 0.00ml @09:36:51.73 Exhaust.select :close @09:36:51.84 Sampled 150.0ml @09:36:51.85 collectSample: Complete @09:36:51.86 Drying puck before fixing for 04:00.00 @09:36:51.87 CBV.dial :waste2b @09:36:52.66 delay 1:00 @09:36:54.83 Sleepy.power :sampler,:OFF @09:37:31.14 delay 3 seconds @09:37:34.15 ATV.dial :air3 @09:37:34.50 delay 3 seconds @09:37:37.56 AS.seek 0.734ml @09:37:42.97 delay 3 seconds @09:37:45.98 ATV.dial :bleach @09:37:46.38 AS.seek 0.719ml @09:37:49.69 delay 3 seconds @09:37:52.66 CSV.dial :air @09:37:52.70 ATV.dial :AIV @09:37:53.11 CS.reconfigure CSfast @09:37:53.14 CS.seek 10.000ml @09:37:53.78 AS.reconfigure ASconfig @09:37:53.82 AS.seek 0.015ml @09:38:16.94 CBV.dial between CSV and waste2b @09:38:17.26 Pumping wcr puck from tube 2 up to 55psi... @09:38:17.31 CBV.dial SSV @09:38:18.76 CBV.dial between CSV and waste2b @09:38:19.68 CSV.dial between air and CTV @09:38:20.08 CS.seek 6.730ml @09:38:28.36 CTV.dial CSV,avoiding => air @09:38:28.38 CSV.dial CTV @09:38:29.14 CS.reconfigure CSpressurize @09:38:29.17 CS.seek 0.000ml @09:38:35.15 ATV.dial :waste @09:38:36.52 AS.seek empty @09:38:37.70 ATV.dial :air3 @09:38:38.27 AS.reconfigure ASslow1 @09:38:38.30 AS.seek 0.009ml @09:38:40.67 delay 2 seconds @09:38:42.68 ATV.dial :bleach @09:38:43.06 AS.seek 0.579ml @09:38:43.84 CBV.dial :waste2b @09:38:44.18 CS.reconfigure CSconfig @09:38:44.21 CS.seek empty @09:38:47.79 delay 1:00 @09:39:47.80 CTV.dial :air,avoiding => intake @09:39:49.08 Fix #1 of 2 (with rnal) for 02:00.00 @09:39:49.09 CS Pulling 2ml of rnal @09:39:49.10 CSV.dial CSR @09:39:49.52 CSR.select :rnal @09:39:49.62 CS.seek 2.000ml @09:39:55.51 delay 5 seconds @09:40:00.52 CSR.select :air @09:40:00.61 CS.reconfigure CSfast @09:40:00.65 CS.seek 10.000ml @09:40:19.85 CBV.dial between CSV and waste2b @09:40:20.17 Pumping wcr puck from tube 2 up to 55psi... @09:40:20.22 CBV.dial SSV @09:40:21.64 CBV.dial between CSV and waste2b @09:40:22.59 CSV.dial between air and CTV @09:40:23.25 CS.seek 6.699ml @09:40:31.58 CTV.dial CSV,avoiding => air @09:40:31.60 CSV.dial CTV @09:40:32.96 CS.reconfigure CSpressurize @09:40:32.99 CS.seek 0.000ml @09:40:39.52 delay 3 seconds @09:40:42.53 ATV.dial :air3 @09:40:42.88 delay 3 seconds @09:40:45.94 AS.seek 0.604ml @09:40:49.59 delay 2:00 @09:40:51.30 delay 3 seconds @09:40:54.31 ATV.dial :bleach @09:40:54.69 AS.seek 0.589ml @09:40:57.97 delay 3 seconds @09:41:00.98 ATV.dial :ASV @09:41:01.94 ASV.dial :wasteR @09:41:02.89 AS.seek 0.000ml @09:42:49.60 CBV.dial :waste2b @09:42:49.92 CS.reconfigure CSconfig @09:42:49.95 CS.seek empty @09:42:51.47 delay 1:00 @09:43:03.44 delay 1:00 @09:43:51.48 CTV.dial :air,avoiding => intake @09:43:52.76 Fix #2 of 2 (with rnal) for 02:00.00 @09:43:52.77 CS Pulling 2ml of rnal CSV.dial CSR @09:43:53.89 CSR.select :rnal @09:43:53.99 CS.seek 2.000ml @09:43:59.90 delay 5 seconds @09:44:03.45 AFV.select :carrierSolution @09:44:03.54 delay 2 seconds @09:44:04.91 CSR.select :air @09:44:05.01 CS.reconfigure CSfast @09:44:05.57 AS.reconfigure ASconfig @09:44:05.60 CS.seek 10.000ml @09:44:05.65 AS.seek 1.000ml @09:44:24.87 CBV.dial between CSV and waste2b @09:44:25.19 Pumping wcr puck from tube 2 up to 55psi... @09:44:25.24 CBV.dial SSV @09:44:26.67 CBV.dial between CSV and waste2b @09:44:27.59 CSV.dial between air and CTV @09:44:28.16 CS.seek 6.596ml @09:44:36.82 CTV.dial CSV,avoiding => air @09:44:36.84 CSV.dial CTV @09:44:38.21 CS.reconfigure CSpressurize @09:44:38.24 CS.seek 0.000ml @09:44:54.39 delay 2:00 @09:45:04.23 delay 1 second @09:45:05.24 AFV.select :holdingCoil @09:45:05.33 delay 1 second @09:45:06.34 ATV.dial :ASV @09:45:06.35 ASV.dial :wasteR @09:45:06.36 AS.reconfigure ASslow1 @09:45:06.39 AS.seek 0.000ml @09:46:54.40 CBV.dial :waste2b @09:46:54.72 CS.reconfigure CSconfig @09:46:54.75 CS.seek empty @09:46:56.46 delay 1:00 @09:47:56.47 CTV.dial :air,avoiding => intake @09:47:57.79 Storing wcr puck in Collection Clamp to tube 1 @09:47:57.93 SC.reconfigure SCtight @09:47:57.96 SC.seek 1 @09:48:00.21 SC.reconfigure SCloose @09:48:00.39 Elbow.reconfigure TightElbow @09:48:00.42 Elbow.seek 1 @09:48:02.04 Forearm.reconfigure TightForearm @09:48:02.07 Forearm.seek 1 + 800 counts @09:48:03.26 SE.reconfigure SEpick @09:48:03.31 SE.seek beamBreak @09:48:05.25 SE.configure SEconfig @09:48:05.37 Elbow.reconfigure LimpElbow @09:48:05.41 Forearm.reconfigure LooseForearm @09:48:05.45 Forearm.seek 1 @09:48:05.69 Elbow.hold @09:48:05.74 Elbow.reconfigure TightElbow @09:48:05.82 SE.seek 10.05 Pucks below Top Plate @09:48:06.18 Forearm.coast @09:48:06.22 Forearm.seek 1 + 344 counts @09:48:06.46 Forearm.reconfigure TightForearm @09:48:06.49 Forearm.seek 1 + 800 counts @09:48:06.75 SE.seek 11.05 Pucks below Top Plate @09:48:07.42 Forearm.reconfigure LooseForearm @09:48:07.46 Forearm.seek retract @09:48:08.40 Elbow.seek CC @09:48:09.96 Elbow.reconfigure LooseElbow @09:48:10.04 Forearm.seek CC @09:48:10.98 CC.seek sealed @09:48:11.92 Hand.grip @09:48:12.64 CC.seek open @09:48:16.00 Forearm.seek retract @09:48:16.95 Elbow.reconfigure TightElbow @09:48:16.98 Elbow.seek 1 @09:48:18.56 Elbow.reconfigure LimpElbow @09:48:18.61 Forearm.seek 1 @09:48:19.64 Elbow.hold @09:48:19.69 Elbow.reconfigure TightElbow @09:48:19.73 Hand.release @09:48:20.59 Forearm.coast @09:48:21.22 Forearm.seek 1 + 311 counts @09:48:21.46 Forearm.reconfigure TightForearm @09:48:21.50 Forearm.seek 1 + 800 counts @09:48:21.80 SE.seek down @09:48:22.90 Forearm.reconfigure LooseForearm @09:48:22.93 Forearm.seek retract @09:48:23.51 SE.coast @09:48:23.88 SC.coast @09:48:23.91 Starting finalPurge @09:48:23.92 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @09:48:24.08 Elbow.seek FlushPuck::Garage @09:48:25.85 Elbow.reconfigure LooseElbow @09:48:25.93 Forearm.seek garage @09:48:26.99 Hand.grip @09:48:27.85 Forearm.seek retract @09:48:28.86 Elbow.reconfigure TightElbow @09:48:28.89 Elbow.seek CC @09:48:30.63 delay 20 seconds @09:48:31.65
Elbow.reconfigure LooseElbow @09:48:31.73 Forearm.reconfigure TightForearm @09:48:31.76 Forearm.seek CC @09:48:32.93 CC.seek closed @09:48:36.69 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.96 @09:48:36.75 Hand.release @09:48:37.55 Forearm.reconfigure LooseForearm @09:48:37.58 Forearm.seek retract @09:48:38.53 Flushing the Collection Valve::Manifold @09:48:38.63 CS Pulling 5ml of flush @09:48:38.64 CSV.dial CSR @09:48:39.14 CSR.select :flush @09:48:39.24 CS.seek 5.000ml @09:48:50.64 ATV.dial :air3 @09:48:51.77 AS.seek 0.590ml @09:48:52.77
delay 5 seconds @09:48:57.78 CSR.select :air @09:48:57.92 CS.reconfigure CSfast @09:48:57.95 CS.seek 7.500ml @09:49:04.52 CSV.dial :waste @09:49:05.78 CS.reconfigure CSconfig @09:49:05.81 CS.seek 0.000ml @09:49:25.83 Flushing lower manifold's waste1 port @09:49:25.93 CSV.dial :flush @09:49:26.37 CS.reconfigure CSfast @09:49:26.40 CS.seek 5.000ml @09:49:38.70 delay 5 seconds @09:49:43.71 CSV.dial :air @09:49:44.20 CS.seek 7.000ml @09:49:49.64 CSV.dial CTV @09:49:49.66
CTV.dial CSV @09:49:50.98 CBV.dial :waste1 @09:49:51.73 CS.seek 0.000ml @09:50:08.39 Flushing lower manifold's waste2b port @09:50:08.48 CSV.dial :flush @09:50:09.27 CS.seek 5.000ml @09:50:21.57 delay 5 seconds @09:50:26.58 CSV.dial :air @09:50:27.12 CS.seek 7.000ml @09:50:32.58 CSV.dial CTV @09:50:32.59
CTV.dial CSV @09:50:33.03 CBV.dial :waste2b @09:50:33.79 CS.seek 0.000ml @09:50:50.39 Flushing upper manifold @09:50:50.48 CSV.dial :flush @09:50:51.27 CS.seek 5.000ml @09:50:52.41 delay 3 seconds @09:50:56.02 ATV.dial :ASV @09:50:57.09 ASV.dial :wasteR @09:50:57.15 AS.seek 0.090ml @09:51:03.57
delay 5 seconds @09:51:08.58 CSV.dial :air @09:51:09.12 CS.seek 7.000ml @09:51:14.57 CSV.dial CBV @09:51:14.59
CBV.dial CSV @09:51:15.71 CTV.dial :air @09:51:16.97 CS.seek 0.000ml @09:51:33.54 delay 7 seconds @09:51:40.55 CS.seek 3.000ml @09:51:48.32 CSV.dial :waste @09:51:48.83 CS.seek 0.000ml @09:51:56.54 CSV.dial :flush @09:51:56.97 CS.seek 3.000ml @09:52:04.73 delay 5 seconds @09:52:09.74 CSV.dial :air @09:52:10.21 CS.seek 5.000ml @09:52:15.63 CSV.dial :air @09:52:15.65 CS.seek 0.000ml @09:52:27.73 delay 7 seconds @09:52:34.74 CS.seek 3.000ml @09:52:39.39 delay 30 seconds @09:52:42.48
CSV.dial :waste @09:52:43.28 CS.seek 0.000ml @09:52:50.88 Disinfecting external Intake with mfbkill @09:52:50.97 CSV.dial CSR @09:52:52.22 CSR.select :mfbkill @09:52:52.32 CS.seek 8.000ml @09:53:09.40 ATV.dial :extSrc @09:53:09.92 delay 3 seconds @09:53:11.41
delay 5 seconds @09:53:12.93 ATV.dial :ElutionWater @09:53:13.95 AS.seek 0.120ml @09:53:16.42
CSR.select :air @09:53:16.51 CS.seek 10.000ml @09:53:20.34 ATV.dial :air3 @09:53:20.72 AS.seek 0.130ml @09:53:21.91 CSV.dial CBV @09:53:21.93
CBV.dial CSV @09:53:23.01 CTV.dial :intake @09:53:23.05 delay 2 seconds @09:53:23.71
Intake.select :open @09:53:23.81 CS.seek 2.000ml @09:53:25.05 ATV.dial :bleach @09:53:25.42 AS.seek 0.230ml @09:53:43.16
Intake.select :close @09:53:43.25 CTV.dial :air @09:53:44.00 CS.reconfigure CSconfig @09:53:44.03 CS.seek 0.000ml @09:53:46.07 delay 3 seconds @09:53:49.08 ATV.dial :air3 @09:53:49.42 delay 3 seconds @09:53:50.13
Flushing lower manifold's waste1 port @09:53:50.14 CS Pulling 4ml of mfbkill @09:53:50.15 CSV.dial CSR @09:53:51.19 CSR.select :mfbkill @09:53:51.29 CS.seek 4.000ml @09:53:52.48 AS.seek 0.255ml @09:53:57.85 delay 3 seconds @09:54:00.86 ATV.dial :bleach @09:54:01.25 AS.seek 0.240ml @09:54:02.22
delay 5 seconds @09:54:04.53 delay 3 seconds @09:54:07.23
CSR.select :air @09:54:07.32 CS.reconfigure CSfast @09:54:07.36 CS.seek 10.000ml @09:54:07.54 ATV.dial :ASV @09:54:08.53 ASV.dial :SPEdelivery @09:54:08.85 AFV.select :carrierSolution @09:54:08.95 delay 2 seconds @09:54:10.96 AS.reconfigure ASconfig @09:54:10.99 AS.seek 0.500ml @09:54:21.89 CSV.dial CTV @09:54:21.91
CTV.dial CSV @09:54:23.25 CBV.dial :waste1 @09:54:23.71 CS.seek 0.000ml @09:54:26.43 delay 1 second @09:54:27.44 AFV.select :holdingCoil @09:54:27.58 AS.reconfigure ASslow1 @09:54:27.61 AS.seek 0.170ml @09:54:47.06
Flushing lower manifold's waste2b port @09:54:47.07 CS Pulling 4ml of mfbkill @09:54:47.08 CSV.dial CSR @09:54:47.58 CSR.select :mfbkill @09:54:47.68 CS.reconfigure CSconfig @09:54:47.72 CS.seek 4.000ml @09:54:58.84 delay 5 seconds @09:55:03.85 CSR.select :air @09:55:03.94 CS.reconfigure CSfast @09:55:03.98 CS.seek 10.000ml @09:55:18.54 CSV.dial CTV @09:55:18.56
CTV.dial CSV @09:55:19.07 CBV.dial :waste2b @09:55:19.81 CS.seek 0.000ml @09:55:35.18 SPE.hold 2:46:40,95.0C @09:55:35.21 delay 3:00 @09:55:43.28
Killing upper manifold @09:55:43.29 CS Pulling 4ml of mfbkill @09:55:43.30 CSV.dial CSR @09:55:44.37 CSR.select :mfbkill @09:55:44.47 CS.reconfigure CSconfig @09:55:44.51 CS.seek 4.000ml @09:55:55.41 delay 5 seconds @09:56:00.42 CSR.select :air @09:56:00.51 CS.reconfigure CSfast @09:56:00.55 CS.seek 10.000ml @09:56:15.57 CSV.dial CBV @09:56:15.59
CBV.dial CSV @09:56:16.66 CTV.dial :air @09:56:17.97 CS.seek 0.000ml @09:56:42.13 delay 7 seconds @09:56:49.14 CS.seek 3.000ml @09:56:57.10 CSV.dial :waste @09:56:57.55 CS.seek 0.000ml @09:57:05.54 Flushing upper manifold @09:57:05.64 CSV.dial :flush @09:57:06.09 CS.seek 5.000ml @09:57:18.72 delay 5 seconds @09:57:23.73 CSV.dial :air @09:57:24.21 CS.seek 7.001ml @09:57:29.72 CSV.dial CBV @09:57:29.74
CBV.dial CSV @09:57:30.86 CTV.dial :air @09:57:30.87 CS.seek 0.000ml @09:57:48.00 delay 7 seconds @09:57:55.01 CS.seek 3.000ml @09:58:02.91 CSV.dial :waste @09:58:03.43 CS.seek 0.000ml @09:58:11.34 CSV.dial :flush @09:58:11.78 CS.seek 3.000ml @09:58:19.72 delay 5 seconds @09:58:24.73 CSV.dial :air @09:58:25.22 CS.seek 5.000ml @09:58:30.75 CSV.dial :air @09:58:30.77 CS.seek 0.000ml @09:58:35.24 AFV.select :carrierSolution @09:58:35.33 delay 2 seconds @09:58:37.34 AS.reconfigure ASconfig @09:58:37.37 AS.seek 1.000ml @09:58:43.41
delay 7 seconds @09:58:50.42 CS.seek 3.000ml @09:58:58.37 CSV.dial :waste @09:58:59.18 CS.seek 0.000ml @09:59:06.94 Flushing lower manifold's waste1 port @09:59:07.04 CSV.dial :flush @09:59:07.57 CS.seek 5.000ml @09:59:20.09 delay 5 seconds @09:59:25.10 CSV.dial :air @09:59:25.56 CS.seek 7.000ml @09:59:26.04 delay 1 second @09:59:27.05 AFV.select :holdingCoil @09:59:27.14 delay 1 second @09:59:28.15 ASV.dial :wasteD @09:59:29.16 AS.seek 0.000ml @09:59:31.07 CSV.dial CTV @09:59:31.08
CTV.dial CSV @09:59:32.44 CBV.dial :waste1 @09:59:32.91 CS.seek 0.000ml @09:59:49.78 Flushing lower manifold's waste2b port @09:59:49.87 CSV.dial :flush @09:59:50.68 CS.seek 5.000ml @10:00:03.12 delay 5 seconds @10:00:08.13 CSV.dial :air @10:00:09.09 CS.seek 7.000ml @10:00:14.56 CSV.dial CTV @10:00:14.58
CTV.dial CSV @10:00:15.03 CBV.dial :waste2b @10:00:15.80 CS.seek 0.000ml @10:00:27.79 Priming Eluate @10:00:27.80 AFV.select :carrierSolution @10:00:27.89 delay 2 seconds @10:00:29.90 AS.seek 0.500ml @10:00:32.57
Disinfecting external Intake with kill @10:00:32.66 CSV.dial CSR @10:00:33.07 CSR.select :kill @10:00:33.17 CS.seek 8.000ml @10:00:52.53 delay 5 seconds @10:00:57.54 CSR.select :air @10:00:57.63 CS.seek 10.000ml @10:00:59.32 delay 1 second @10:01:00.33 AFV.select :holdingCoil @10:01:00.42 ATV.dial ASV @10:01:00.43 ASV.dial :SPErecovery @10:01:01.08 Swish #1 of 2 @10:01:01.14 AS.reconfigure ASslow0 @10:01:01.17 AS.seek 0.600ml @10:01:03.15 CSV.dial CBV @10:01:03.17
CBV.dial CSV @10:01:04.21 CTV.dial :intake @10:01:04.96 Intake.select :open @10:01:05.06 CS.seek 2.000ml @10:01:11.83 AS.seek 0.500ml @10:01:22.30 Swish #2 of 2 @10:01:22.36 AS.seek 0.600ml @10:01:24.31
Intake.select :close @10:01:24.40 CTV.dial :air @10:01:25.13 CS.reconfigure CSconfig @10:01:25.16 CS.seek 0.000ml @10:01:31.05 CSV.dial CSR @10:01:32.12 CSR.select :flush @10:01:32.22 CS.seek 5.000ml @10:01:33.54 AS.seek 0.500ml @10:01:44.02 delay 3 seconds @10:01:45.68
delay 5 seconds @10:01:47.08 AS.reconfigure ASslow1 @10:01:47.11 AS.seek 0.676ml @10:01:50.69
CSR.select :air @10:01:50.78 CS.seek 10.000ml @10:02:04.21 CSV.dial CBV @10:02:05.67 CS.seek 0.000ml @10:02:23.30 delay 3 seconds @10:02:26.31 Eluate primed @10:02:26.32 ATV.dial :waste2 @10:02:26.86 AS.reconfigure ASnorm @10:02:26.89 AS.seek 0.000ml @10:02:31.80
Garaging FlushPuck in Collection Clamp @10:02:31.86 Elbow.reconfigure TightElbow @10:02:31.89 Elbow.seek CC @10:02:31.99 Elbow.reconfigure LooseElbow @10:02:32.07 Forearm.seek CC @10:02:33.03 CC.seek sealed @10:02:33.96 Hand.grip @10:02:34.73 CC.seek open @10:02:38.03 Forearm.seek retract @10:02:38.97 Elbow.reconfigure TightElbow @10:02:39.00 Elbow.seek garage @10:02:41.81 Elbow.reconfigure LooseElbow @10:02:41.89 Forearm.reconfigure TightForearm @10:02:41.92 Forearm.seek garage @10:02:43.10 Hand.release @10:02:43.90 Forearm.reconfigure LooseForearm @10:02:43.93 Forearm.seek retract @10:03:01.77 SPE.coast @10:03:01.84 ASV.dial :SPErecovery @10:03:01.86 Setting up PCR for HF183em -- writing /var/log/esp/HF183em14mar0710h170ml.pcr @10:03:01.87 Building PCR Slug from ASV with MM2 mix, PP4 primer @10:03:01.88 Priming with carrier @10:03:01.89 ATV.dial ARV @10:03:02.60 ARV.dial :carrier @10:03:02.90 AS.seek 0.500ml @10:03:28.83 AFV.select :carrierSolution @10:03:28.92 delay 2 seconds @10:03:30.94 AS.reconfigure ASconfig @10:03:30.97 AS.seek 1.000ml @10:04:00.42 delay 1 second @10:04:01.43 AFV.select :holdingCoil @10:04:01.52 delay 1 second @10:04:02.53 ATV.dial :waste @10:04:03.89 AS.reconfigure ASnorm @10:04:03.92 AS.seek empty @10:04:55.37 AFV.select :carrierSolution @10:04:55.47 delay 2 seconds @10:04:57.48 AS.reconfigure ASconfig @10:04:57.51 AS.seek 0.500ml @10:05:27.01 delay 1 second @10:05:28.02 AFV.select :holdingCoil @10:05:28.11 Building Slug @10:05:28.12 ATV.dial :ARV @10:05:29.47 delay 2 seconds @10:05:31.48 ARV.dial :air1 @10:05:31.83 delay 2 seconds @10:05:33.89 AS.reconfigure ASslow2 @10:05:33.92 AS.seek 0.506ml @10:05:40.28 delay 2 seconds @10:05:42.29 ARV.dial :MM2 @10:05:43.63 delay 2 seconds @10:05:45.63 Adding MM2 @10:05:45.69 AS.seek 0.524ml @10:06:04.31 delay 25 seconds @10:06:29.32 ARV.dial :PP4 @10:06:29.66 Adding PP4 @10:06:29.67 delay 2 seconds @10:06:31.72 AS.seek 0.530ml @10:06:38.21 delay 10 seconds @10:06:48.22 ARV.dial :air3 @10:06:48.55 adding ARV air @10:06:48.56 delay 2 seconds @10:06:50.61 AS.seek 0.536ml @10:06:57.06 delay 5 seconds @10:07:02.07 ARV.dial :carrier @10:07:03.10 delay 7 seconds @10:07:10.16 AS.seek 0.594ml @10:08:09.49 delay 5 seconds @10:08:14.50 ATV.dial :ASV @10:08:14.82 delay 2 seconds @10:08:16.88 AS.seek 0.600ml @10:08:23.24 delay 4 seconds @10:08:27.25 ATV.dial ARV @10:08:27.68 delay 2 seconds @10:08:29.69 ARV.dial :carrier @10:08:29.70 delay 2 seconds @10:08:31.75 AS.seek 0.650ml @10:09:23.12 delay 4 seconds @10:09:27.13 ATV.dial PCR @10:09:27.65 ASV.dial :waste1 @10:09:29.18 ARV.dial :air1 @10:09:29.59 Positioning Slug by 0.324ml @10:09:29.64 AS.seek 0.326ml @10:15:00.51 delay 5 seconds @10:15:05.52 ATV.dial ARV @10:15:06.04 ASV.dial :PCRlock @10:15:06.54 delay 5 seconds @10:15:11.55 Running HF183em ProfileEM60C2 thermocycling @10:15:11.56 PCR.program ProfileEM60C2 @10:15:11.67 ProfileEM60C2 should complete in < 2:11:38 @10:15:11.68 PCR.run 2:49:32.5 @12:10:10.71 PCR.off @12:10:11.24 PCR for HF183em completed -- closed /var/log/esp/HF183em14mar0710h170ml.pcr @12:10:11.29 Cleaning PCR @12:10:11.30 AFV.select :carrierSolution @12:10:11.39 delay 2 seconds @12:10:13.40 AS.reconfigure ASconfig @12:10:13.43 AS.seek 0.500ml @12:10:23.87 delay 1 second @12:10:24.88 AFV.select :holdingCoil @12:10:24.97 ASV.dial :waste1 @12:10:25.45 ATV.dial :air3 @12:10:26.39 AS.reconfigure ASslow1 @12:10:26.42 AS.seek 0.510ml @12:10:28.71 delay 2 seconds @12:10:30.71 ATV.dial :bleach @12:10:31.19 AS.seek 0.810ml @12:11:32.59 delay 3 seconds @12:11:35.60 ATV.dial :air3 @12:11:35.92 delay 3 seconds @12:11:38.98 AS.seek 0.835ml @12:11:44.33 delay 3 seconds @12:11:47.34 ATV.dial :bleach @12:11:47.73 AS.seek 0.820ml @12:11:51.06 delay 3 seconds @12:11:54.07 ARV.dial :waste @12:11:54.58 delay 3 seconds @12:11:57.59 ATV.dial :ARV @12:11:58.32 AS.reconfigure ASconfig @12:11:58.36 AS.seek 0.470ml @12:12:19.04 AFV.select :carrierSolution @12:12:19.13 delay 2 seconds @12:12:21.14 AS.seek 1.000ml @12:12:52.36 delay 1 second @12:12:53.37 AFV.select :holdingCoil @12:12:53.46 delay 1 second @12:12:54.47 AS.reconfigure ASfast @12:12:54.50 AS.seek 0.000ml @12:13:22.24 AFV.select :carrierSolution @12:13:22.33 delay 2 seconds @12:13:24.34 AS.reconfigure ASconfig @12:13:24.37 AS.seek 1.000ml @12:14:23.00 delay 1 second @12:14:24.01 AFV.select :holdingCoil @12:14:24.25 delay 1 second @12:14:25.26 AS.reconfigure ASfast @12:14:25.29 AS.seek 0.000ml @12:14:53.03 AFV.select :carrierSolution @12:14:53.12 delay 2 seconds @12:14:55.13 AS.reconfigure ASconfig @12:14:55.76 AS.seek 1.000ml @12:15:54.39 delay 1 second @12:15:55.40 AFV.select :holdingCoil @12:15:55.49 delay 1 second @12:15:56.50 AS.reconfigure ASfast @12:15:56.53 AS.seek 0.000ml @12:16:24.26 ARV.dial between carrier and air1 @12:16:24.78 ATV.dial :air3 @12:16:25.72 AS.reconfigure ASslow1 @12:16:25.75 AS.seek 0.010ml @12:16:28.09 delay 2 seconds @12:16:30.10 ATV.dial :bleach @12:16:30.49 AS.seek 0.810ml @12:19:13.91 delay 3 seconds @12:19:16.92 ATV.dial :air3 @12:19:17.26 delay 3 seconds @12:19:20.32 AS.seek 0.835ml @12:19:25.65 delay 3 seconds @12:19:28.66 ATV.dial :bleach @12:19:29.06 AS.seek 0.820ml @12:19:32.37 delay 3 seconds @12:19:35.38 ATV.dial PCR @12:19:35.71 AS.reconfigure ASconfig @12:19:35.74 AS.seek empty @12:20:23.80 PCR.program :bleachProfile @12:20:23.85 PCR program should complete in < 2:45 @12:20:23.86 PCR.run 10:00 @12:23:25.07 PCR.off @12:23:25.10 AFV.select :carrierSolution @12:23:25.19 delay 2 seconds @12:23:27.20 AS.seek 1.000ml @12:24:25.86 delay 1 second @12:24:26.87 AFV.select :holdingCoil @12:24:26.96 delay 1 second @12:24:27.97 AS.reconfigure ASfast @12:24:28.00 AS.seek 0.000ml @12:24:55.73 delay 3 seconds @12:24:58.74 AFV.select :carrierSolution @12:24:58.83 delay 2 seconds @12:25:00.84 AS.reconfigure ASconfig @12:25:00.87 AS.seek 1.000ml @12:25:59.48 delay 1 second @12:26:00.49 AFV.select :holdingCoil @12:26:00.58 delay 1 second @12:26:01.59 AS.reconfigure ASfast @12:26:01.62 AS.seek 0.000ml @12:26:29.37 delay 3 seconds @12:26:32.38 AFV.select :carrierSolution @12:26:32.47 delay 2 seconds @12:26:34.48 AS.reconfigure ASconfig @12:26:34.51 AS.seek 1.000ml @12:27:33.07 delay 1 second @12:27:34.08 AFV.select :holdingCoil @12:27:34.25 delay 1 second @12:27:35.26 AS.reconfigure ASfast @12:27:35.29 AS.seek 0.000ml @12:28:02.98 delay 3 seconds @12:28:05.99 AFV.select :carrierSolution @12:28:06.08 delay 2 seconds @12:28:08.09 AS.reconfigure ASconfig @12:28:08.12 AS.seek 1.000ml @12:29:06.69 delay 1 second @12:29:07.70 AFV.select :holdingCoil @12:29:07.79 delay 1 second @12:29:08.80 AS.reconfigure ASfast @12:29:08.83 AS.seek 0.000ml @12:29:36.58 delay 3 seconds @12:29:39.59 AFV.select :carrierSolution @12:29:39.68 delay 2 seconds @12:29:41.69 AS.reconfigure ASconfig @12:29:41.72 AS.seek 1.000ml @12:30:40.34 delay 1 second @12:30:41.35 AFV.select :holdingCoil @12:30:41.44 delay 1 second @12:30:42.45 AS.reconfigure ASnorm @12:30:42.48 AS.seek 0.000ml @12:31:33.96 Setting up PCR for ENTap -- writing /var/log/esp/ENTap14mar0712h170ml.pcr @12:31:33.97 Building PCR Slug from ASV with MM1 mix, PP1 primer @12:31:33.98 Priming with carrier @12:31:33.99 ATV.dial ARV @12:31:34.51 ARV.dial :carrier @12:31:34.78 AS.seek 0.500ml @12:32:00.68 AFV.select :carrierSolution @12:32:00.77 delay 2 seconds @12:32:02.78 AS.reconfigure ASconfig @12:32:02.82 AS.seek 1.000ml @12:32:32.24 delay 1 second @12:32:33.25 AFV.select :holdingCoil @12:32:33.34 delay 1 second @12:32:34.35 ATV.dial :waste @12:32:35.71 AS.reconfigure ASnorm @12:32:35.74 AS.seek empty @12:33:27.21 AFV.select :carrierSolution @12:33:27.31 delay 2 seconds @12:33:29.32 AS.reconfigure ASconfig @12:33:29.35 AS.seek 0.500ml @12:33:58.82 delay 1 second @12:33:59.83 AFV.select :holdingCoil @12:33:59.92 Building Slug @12:33:59.93 ATV.dial :ARV @12:34:01.18 delay 2 seconds @12:34:03.19 ARV.dial :air1 @12:34:03.52 delay 2 seconds @12:34:05.58 AS.reconfigure ASslow2 @12:34:05.61 AS.seek 0.506ml @12:34:12.00 delay 2 seconds @12:34:14.01 ARV.dial :MM1 @12:34:14.58 delay 2 seconds @12:34:16.59 Adding MM1 @12:34:16.65 AS.seek 0.524ml @12:34:35.52 delay 25 seconds @12:35:00.53 ARV.dial :PP1 @12:35:00.86 Adding PP1 @12:35:00.87 delay 2 seconds @12:35:02.92 AS.seek 0.530ml @12:35:09.30 delay 10 seconds @12:35:19.31 ARV.dial :air1 @12:35:19.64 adding ARV air @12:35:19.65 delay 2 seconds @12:35:21.70 AS.seek 0.536ml @12:35:27.99 delay 5 seconds @12:35:33.00 ARV.dial :carrier @12:35:33.31 delay 7 seconds @12:35:40.37 AS.seek 0.594ml @12:36:40.19 delay 5 seconds @12:36:45.20 ATV.dial :ASV @12:36:45.53 delay 2 seconds @12:36:47.59 AS.seek 0.600ml @12:36:53.95 delay 4 seconds @12:36:57.96 ATV.dial ARV @12:36:58.28 delay 2 seconds @12:37:00.29 ARV.dial :carrier @12:37:00.30 delay 2 seconds @12:37:02.35 AS.seek 0.650ml @12:37:53.72 delay 4 seconds @12:37:57.73 ATV.dial PCR @12:37:58.25 ASV.dial :waste1 @12:37:58.26 ARV.dial :air1 @12:37:58.60 Positioning Slug by 0.324ml @12:37:58.65 AS.seek 0.326ml @12:43:29.35 delay 5 seconds @12:43:34.36 ATV.dial ARV @12:43:34.99 ASV.dial :PCRlock @12:43:35.49 delay 5 seconds @12:43:40.50 Running ENTap Profile60C thermocycling @12:43:40.51 PCR.program Profile60C @12:43:40.61 Profile60C should complete in < 1:39:08 @12:43:40.62 PCR.run 2:08:55 @14:06:25.37 PCR.off @14:06:25.88 PCR for ENTap completed -- closed /var/log/esp/ENTap14mar0712h170ml.pcr @14:06:25.93 Cleaning PCR @14:06:25.94 AFV.select :carrierSolution @14:06:26.03 delay 2 seconds @14:06:28.04 AS.reconfigure ASconfig @14:06:28.07 AS.seek 0.500ml @14:06:38.48 delay 1 second @14:06:39.49 AFV.select :holdingCoil @14:06:39.58 ASV.dial :waste1 @14:06:40.32 ATV.dial :air3 @14:06:41.24 AS.reconfigure ASslow1 @14:06:41.28 AS.seek 0.510ml @14:06:43.57 delay 2 seconds @14:06:45.58 ATV.dial :bleach @14:06:45.95 AS.seek 0.810ml @14:07:47.39 delay 3 seconds @14:07:50.40 ATV.dial :air3 @14:07:50.82 delay 3 seconds @14:07:53.88 AS.seek 0.835ml @14:07:59.20 delay 3 seconds @14:08:02.21 ATV.dial :bleach @14:08:02.70 AS.seek 0.820ml @14:08:06.01 delay 3 seconds @14:08:09.02 ARV.dial :waste @14:08:09.53 delay 3 seconds @14:08:12.54 ATV.dial :ARV @14:08:13.29 AS.reconfigure ASconfig @14:08:13.32 AS.seek 0.470ml @14:08:34.60 AFV.select :carrierSolution @14:08:34.69 delay 2 seconds @14:08:36.70 AS.seek 1.000ml @14:09:07.91 delay 1 second @14:09:08.92 AFV.select :holdingCoil @14:09:09.01 delay 1 second @14:09:10.02 AS.reconfigure ASfast @14:09:10.05 AS.seek 0.000ml @14:09:37.80 AFV.select :carrierSolution @14:09:37.90 delay 2 seconds @14:09:39.91 AS.reconfigure ASconfig @14:09:39.94 AS.seek 1.000ml @14:10:38.54 delay 1 second @14:10:39.55 AFV.select :holdingCoil @14:10:39.64 delay 1 second @14:10:40.65 AS.reconfigure ASfast @14:10:40.68 AS.seek 0.000ml @14:11:08.43 AFV.select :carrierSolution @14:11:08.53 delay 2 seconds @14:11:10.54 AS.reconfigure ASconfig @14:11:10.57 AS.seek 1.000ml @14:12:09.18 delay 1 second @14:12:10.19 AFV.select :holdingCoil @14:12:10.28 delay 1 second @14:12:11.29 AS.reconfigure ASfast @14:12:11.32 AS.seek 0.000ml @14:12:39.06 ARV.dial between carrier and air1 @14:12:39.48 ATV.dial :air3 @14:12:40.40 AS.reconfigure ASslow1 @14:12:40.43 AS.seek 0.010ml @14:12:42.78 delay 2 seconds @14:12:44.79 ATV.dial :bleach @14:12:45.17 AS.seek 0.810ml @14:15:28.57 delay 3 seconds @14:15:31.58 ATV.dial :air3 @14:15:31.93 delay 3 seconds @14:15:34.99 AS.seek 0.835ml @14:15:40.29 delay 3 seconds @14:15:43.30 ATV.dial :bleach @14:15:43.69 AS.seek 0.820ml @14:15:47.00 delay 3 seconds @14:15:50.01 ATV.dial PCR @14:15:50.33 AS.reconfigure ASconfig @14:15:50.36 AS.seek empty @14:16:38.49 PCR.program :bleachProfile @14:16:38.54 PCR program should complete in < 2:45 @14:16:38.55 PCR.run 10:00 @14:19:36.74 PCR.off @14:19:36.77 AFV.select :carrierSolution @14:19:36.86 delay 2 seconds @14:19:38.87 AS.seek 1.000ml @14:20:37.53 delay 1 second @14:20:38.54 AFV.select :holdingCoil @14:20:38.63 delay 1 second @14:20:39.64 AS.reconfigure ASfast @14:20:39.67 AS.seek 0.000ml @14:21:07.37 delay 3 seconds @14:21:10.38 AFV.select :carrierSolution @14:21:10.47 delay 2 seconds @14:21:12.48 AS.reconfigure ASconfig @14:21:12.51 AS.seek 1.000ml @14:22:11.12 delay 1 second @14:22:12.13 AFV.select :holdingCoil @14:22:12.22 delay 1 second @14:22:13.23 AS.reconfigure ASfast @14:22:13.26 AS.seek 0.000ml @14:22:41.01 delay 3 seconds @14:22:44.02 AFV.select :carrierSolution @14:22:44.11 delay 2 seconds @14:22:46.12 AS.reconfigure ASconfig @14:22:46.15 AS.seek 1.000ml @14:23:44.69 delay 1 second @14:23:45.70 AFV.select :holdingCoil @14:23:45.79 delay 1 second @14:23:46.80 AS.reconfigure ASfast @14:23:46.83 AS.seek 0.000ml @14:24:14.53 delay 3 seconds @14:24:17.54 AFV.select :carrierSolution @14:24:17.64 delay 2 seconds @14:24:19.65 AS.reconfigure ASconfig @14:24:19.68 AS.seek 1.000ml @14:25:18.28 delay 1 second @14:25:19.29 AFV.select :holdingCoil @14:25:19.38 delay 1 second @14:25:20.39 AS.reconfigure ASfast @14:25:20.42 AS.seek 0.000ml @14:25:48.16 delay 3 seconds @14:25:51.17 AFV.select :carrierSolution @14:25:51.26 delay 2 seconds @14:25:53.27 AS.reconfigure ASconfig @14:25:53.30 AS.seek 1.000ml @14:26:51.90 delay 1 second @14:26:52.91 AFV.select :holdingCoil @14:26:53.00 delay 1 second @14:26:54.01 AS.reconfigure ASnorm @14:26:54.04 AS.seek 0.000ml @14:27:45.51 Setting up PCR for ENTem -- writing /var/log/esp/ENTem14mar0714h170ml.pcr @14:27:45.52 Building PCR Slug from ASV with MM2 mix, PP3 primer @14:27:45.53 Priming with carrier @14:27:45.54 ATV.dial ARV @14:27:46.06 ARV.dial :carrier @14:27:46.34 AS.seek 0.500ml @14:28:12.20 AFV.select :carrierSolution @14:28:12.29 delay 2 seconds @14:28:14.30 AS.reconfigure ASconfig @14:28:14.33 AS.seek 1.000ml @14:28:43.80 delay 1 second @14:28:44.81 AFV.select :holdingCoil @14:28:44.90 delay 1 second @14:28:45.91 ATV.dial :waste @14:28:47.28 AS.reconfigure ASnorm @14:28:47.31 AS.seek empty @14:29:38.77 AFV.select :carrierSolution @14:29:38.87 delay 2 seconds @14:29:40.88 AS.reconfigure ASconfig @14:29:40.91 AS.seek 0.500ml @14:30:10.41 delay 1 second @14:30:11.42 AFV.select :holdingCoil @14:30:11.51 Building Slug @14:30:11.52 ATV.dial :ARV @14:30:12.84 delay 2 seconds @14:30:14.85 ARV.dial :air1 @14:30:15.17 delay 2 seconds @14:30:17.23 AS.reconfigure ASslow2 @14:30:17.26 AS.seek 0.506ml @14:30:23.59 delay 2 seconds @14:30:25.60 ARV.dial :MM2 @14:30:26.81 delay 2 seconds @14:30:28.82 Adding MM2 @14:30:28.88 AS.seek 0.524ml @14:30:47.86 delay 25 seconds @14:31:12.87 ARV.dial :PP3 @14:31:13.20 Adding PP3 @14:31:13.21 delay 2 seconds @14:31:15.27 AS.seek 0.530ml @14:31:21.78 delay 10 seconds @14:31:31.79 ARV.dial :air2 @14:31:32.22 adding ARV air @14:31:32.23 delay 2 seconds @14:31:34.29 AS.seek 0.536ml @14:31:40.83 delay 5 seconds @14:31:45.84 ARV.dial :carrier @14:31:46.87 delay 7 seconds @14:31:53.93 AS.seek 0.594ml @14:32:53.37 delay 5 seconds @14:32:58.38 ATV.dial :ASV @14:32:58.71 delay 2 seconds @14:33:00.77 AS.seek 0.600ml @14:33:07.20 delay 4 seconds @14:33:11.21 ATV.dial ARV @14:33:11.54 delay 2 seconds @14:33:13.55 ARV.dial :carrier @14:33:13.56 delay 2 seconds @14:33:15.61 AS.seek 0.650ml @14:34:06.98 delay 4 seconds @14:34:10.99 ATV.dial PCR @14:34:11.49 ASV.dial :waste1 @14:34:11.50 ARV.dial :air1 @14:34:11.85 Positioning Slug by 0.324ml @14:34:11.90 AS.seek 0.326ml @14:39:42.54 delay 5 seconds @14:39:47.55 ATV.dial ARV @14:39:48.06 ASV.dial :PCRlock @14:39:48.39 delay 5 seconds @14:39:53.40 Running ENTem ProfileEM60C thermocycling @14:39:53.41 PCR.program ProfileEM60C @14:39:53.52 ProfileEM60C should complete in < 2:11:38 @14:39:53.53 PCR.run 2:49:32.5 @16:36:11.76 PCR.off @16:36:12.29 PCR for ENTem completed -- closed /var/log/esp/ENTem14mar0714h170ml.pcr @16:36:12.34 Cleaning PCR @16:36:12.35 AFV.select :carrierSolution @16:36:12.44 delay 2 seconds @16:36:14.45 AS.reconfigure ASconfig @16:36:14.49 AS.seek 0.500ml @16:36:24.93 delay 1 second @16:36:25.94 AFV.select :holdingCoil @16:36:26.03 ASV.dial :waste1 @16:36:26.52 ATV.dial :air3 @16:36:28.02 AS.reconfigure ASslow1 @16:36:28.06 AS.seek 0.510ml @16:36:30.35 delay 2 seconds @16:36:32.36 ATV.dial :bleach @16:36:32.85 AS.seek 0.810ml @16:37:34.27 delay 3 seconds @16:37:37.28 ATV.dial :air3 @16:37:37.61 delay 3 seconds @16:37:40.67 AS.seek 0.835ml @16:37:46.00 delay 3 seconds @16:37:49.01 ATV.dial :bleach @16:37:49.40 AS.seek 0.820ml @16:37:52.72 delay 3 seconds @16:37:55.73 ARV.dial :waste @16:37:56.31 delay 3 seconds @16:37:59.32 ATV.dial :ARV @16:38:00.04 AS.reconfigure ASconfig @16:38:00.08 AS.seek 0.470ml @16:38:20.75 AFV.select :carrierSolution @16:38:20.85 delay 2 seconds @16:38:22.86 AS.seek 1.000ml @16:38:54.08 delay 1 second @16:38:55.09 AFV.select :holdingCoil @16:38:55.18 delay 1 second @16:38:56.19 AS.reconfigure ASfast @16:38:56.22 AS.seek 0.000ml @16:39:23.97 AFV.select :carrierSolution @16:39:24.07 delay 2 seconds @16:39:26.08 AS.reconfigure ASconfig @16:39:26.11 AS.seek 1.000ml @16:40:24.73 delay 1 second @16:40:25.74 AFV.select :holdingCoil @16:40:25.83 delay 1 second @16:40:26.84 AS.reconfigure ASfast @16:40:26.88 AS.seek 0.000ml @16:40:54.62 AFV.select :carrierSolution @16:40:54.72 delay 2 seconds @16:40:56.73 AS.reconfigure ASconfig @16:40:56.76 AS.seek 1.000ml @16:41:55.31 delay 1 second @16:41:56.32 AFV.select :holdingCoil @16:41:56.41 delay 1 second @16:41:57.42 AS.reconfigure ASfast @16:41:57.45 AS.seek 0.000ml @16:42:25.18 ARV.dial between carrier and air1 @16:42:25.59 ATV.dial :air3 @16:42:26.54 AS.reconfigure ASslow1 @16:42:26.57 AS.seek 0.010ml @16:42:28.92 delay 2 seconds @16:42:30.93 ATV.dial :bleach @16:42:31.32 AS.seek 0.810ml @16:45:14.73 delay 3 seconds @16:45:17.74 ATV.dial :air3 @16:45:18.08 delay 3 seconds @16:45:21.14 AS.seek 0.835ml @16:45:26.54 delay 3 seconds @16:45:29.55 ATV.dial :bleach @16:45:30.03 AS.seek 0.820ml @16:45:33.34 delay 3 seconds @16:45:36.35 ATV.dial PCR @16:45:36.68 AS.reconfigure ASconfig @16:45:36.71 AS.seek empty @16:46:24.84 PCR.program :bleachProfile @16:46:24.89 PCR program should complete in < 2:45 @16:46:24.90 PCR.run 10:00 @16:49:23.09 PCR.off @16:49:23.12 AFV.select :carrierSolution @16:49:23.21 delay 2 seconds @16:49:25.22 AS.seek 1.000ml @16:50:23.87 delay 1 second @16:50:24.87 AFV.select :holdingCoil @16:50:24.96 delay 1 second @16:50:25.97 AS.reconfigure ASfast @16:50:26.00 AS.seek 0.000ml @16:50:53.71 delay 3 seconds @16:50:56.72 AFV.select :carrierSolution @16:50:56.81 delay 2 seconds @16:50:58.82 AS.reconfigure ASconfig @16:50:58.85 AS.seek 1.000ml @16:51:57.45 delay 1 second @16:51:58.46 AFV.select :holdingCoil @16:51:58.55 delay 1 second @16:51:59.56 AS.reconfigure ASfast @16:51:59.59 AS.seek 0.000ml @16:52:27.31 delay 3 seconds @16:52:30.32 AFV.select :carrierSolution @16:52:30.41 delay 2 seconds @16:52:32.42 AS.reconfigure ASconfig @16:52:32.45 AS.seek 1.000ml @16:53:31.06 delay 1 second @16:53:32.07 AFV.select :holdingCoil @16:53:32.16 delay 1 second @16:53:33.17 AS.reconfigure ASfast @16:53:33.20 AS.seek 0.000ml @16:54:00.95 delay 3 seconds @16:54:03.96 AFV.select :carrierSolution @16:54:04.05 delay 2 seconds @16:54:06.06 AS.reconfigure ASconfig @16:54:06.09 AS.seek 1.000ml @16:55:04.67 delay 1 second @16:55:05.68 AFV.select :holdingCoil @16:55:05.77 delay 1 second @16:55:06.78 AS.reconfigure ASfast @16:55:06.81 AS.seek 0.000ml @16:55:34.51 delay 3 seconds @16:55:37.52 AFV.select :carrierSolution @16:55:37.61 delay 2 seconds @16:55:39.62 AS.reconfigure ASconfig @16:55:39.65 AS.seek 1.000ml @16:56:38.24 delay 1 second @16:56:39.25 AFV.select :holdingCoil @16:56:39.41 delay 1 second @16:56:40.42 AS.reconfigure ASnorm @16:56:40.45 AS.seek 0.000ml @16:57:31.91 Setting up PCR for IPCap -- writing /var/log/esp/IPCap14mar0716h170ml.pcr @16:57:31.92 Building PCR Slug from ASV with MM1 mix, PP2 primer @16:57:31.93 Priming with carrier @16:57:31.94 ATV.dial ARV @16:57:32.54 ARV.dial :carrier @16:57:32.84 AS.seek 0.500ml @16:57:58.74 AFV.select :carrierSolution @16:57:58.83 delay 2 seconds @16:58:00.84 AS.reconfigure ASconfig @16:58:00.87 AS.seek 1.000ml @16:58:30.30 delay 1 second @16:58:31.31 AFV.select :holdingCoil @16:58:31.40 delay 1 second @16:58:32.41 ATV.dial :waste @16:58:33.67 AS.reconfigure ASnorm @16:58:34.35 AS.seek empty @16:59:25.81 AFV.select :carrierSolution @16:59:25.91 delay 2 seconds @16:59:27.92 AS.reconfigure ASconfig @16:59:27.95 AS.seek 0.500ml @16:59:57.44 delay 1 second @16:59:58.45 AFV.select :holdingCoil @16:59:58.54 Building Slug @16:59:58.55 ATV.dial :ARV @16:59:59.80 delay 2 seconds @17:00:01.81 ARV.dial :air1 @17:00:02.14 delay 2 seconds @17:00:04.20 AS.reconfigure ASslow2 @17:00:04.23 AS.seek 0.506ml @17:00:10.50 delay 2 seconds @17:00:12.51 ARV.dial :MM1 @17:00:13.00 delay 2 seconds @17:00:15.01 Adding MM1 @17:00:15.07 AS.seek 0.524ml @17:00:33.55 delay 25 seconds @17:00:58.56 ARV.dial :PP2 @17:00:58.89 Adding PP2 @17:00:58.91 delay 2 seconds @17:01:00.96 AS.seek 0.530ml @17:01:07.18 delay 10 seconds @17:01:17.19 ARV.dial :air2 @17:01:17.59 adding ARV air @17:01:17.60 delay 2 seconds @17:01:19.66 AS.seek 0.536ml @17:01:25.97 delay 5 seconds @17:01:30.98 ARV.dial :carrier @17:01:32.01 delay 7 seconds @17:01:39.07 AS.seek 0.594ml @17:02:38.46 delay 5 seconds @17:02:43.47 ATV.dial :ASV @17:02:43.89 delay 2 seconds @17:02:45.94 AS.seek 0.600ml @17:02:52.57 delay 4 seconds @17:02:56.58 ATV.dial ARV @17:02:56.91 delay 2 seconds @17:02:58.92 ARV.dial :carrier @17:02:58.93 delay 2 seconds @17:03:00.98 AS.seek 0.650ml @17:03:52.20 delay 4 seconds @17:03:56.21 ATV.dial PCR @17:03:56.70 ASV.dial :waste1 @17:03:56.71 ARV.dial :air1 @17:03:57.06 Positioning Slug by 0.324ml @17:03:57.11 AS.seek 0.326ml @17:09:27.52 delay 5 seconds @17:09:32.53 ATV.dial ARV @17:09:33.04 ASV.dial :PCRlock @17:09:33.52 delay 5 seconds @17:09:38.53 Running IPCap Profile59C thermocycling @17:09:38.54 PCR.program Profile59C @17:09:38.64 Profile59C should complete in < 1:39:08 @17:09:38.65 PCR.run 2:08:55 @18:33:16.10 PCR.off @18:33:16.65 PCR for IPCap completed -- closed /var/log/esp/IPCap14mar0716h170ml.pcr @18:33:16.69 Cleaning PCR @18:33:16.70 AFV.select :carrierSolution @18:33:16.79 delay 2 seconds @18:33:18.80 AS.reconfigure ASconfig @18:33:18.83 AS.seek 0.500ml @18:33:29.27 delay 1 second @18:33:30.28 AFV.select :holdingCoil @18:33:30.37 ASV.dial :waste1 @18:33:30.86 ATV.dial :air3 @18:33:31.80 AS.reconfigure ASslow1 @18:33:31.83 AS.seek 0.510ml @18:33:34.13 delay 2 seconds @18:33:36.14 ATV.dial :bleach @18:33:36.52 AS.seek 0.810ml @18:34:37.95 delay 3 seconds @18:34:40.96 ATV.dial :air3 @18:34:41.28 delay 3 seconds @18:34:44.34 AS.seek 0.835ml @18:34:49.67 delay 3 seconds @18:34:52.68 ATV.dial :bleach @18:34:53.17 AS.seek 0.820ml @18:34:56.48 delay 3 seconds @18:34:59.49 ARV.dial :waste @18:34:59.98 delay 3 seconds @18:35:02.99 ATV.dial :ARV @18:35:03.72 AS.reconfigure ASconfig @18:35:03.76 AS.seek 0.470ml @18:35:24.45 AFV.select :carrierSolution @18:35:24.55 delay 2 seconds @18:35:26.56 AS.seek 1.000ml @18:35:57.79 delay 1 second @18:35:58.80 AFV.select :holdingCoil @18:35:58.89 delay 1 second @18:35:59.90 AS.reconfigure ASfast @18:35:59.93 AS.seek 0.000ml @18:36:27.68 AFV.select :carrierSolution @18:36:27.78 delay 2 seconds @18:36:29.79 AS.reconfigure ASconfig @18:36:29.82 AS.seek 1.000ml @18:37:28.41 delay 1 second @18:37:29.42 AFV.select :holdingCoil @18:37:29.51 delay 1 second @18:37:30.52 AS.reconfigure ASfast @18:37:30.55 AS.seek 0.000ml @18:37:58.24 AFV.select :carrierSolution @18:37:58.33 delay 2 seconds @18:38:00.34 AS.reconfigure ASconfig @18:38:00.37 AS.seek 1.000ml @18:38:58.96 delay 1 second @18:38:59.97 AFV.select :holdingCoil @18:39:00.06 delay 1 second @18:39:01.07 AS.reconfigure ASfast @18:39:01.10 AS.seek 0.000ml @18:39:28.85 ARV.dial between carrier and air1 @18:39:29.25 ATV.dial :air3 @18:39:30.27 AS.reconfigure ASslow1 @18:39:30.30 AS.seek 0.010ml @18:39:32.64 delay 2 seconds @18:39:34.65 ATV.dial :bleach @18:39:35.13 AS.seek 0.810ml @18:42:18.55 delay 3 seconds @18:42:21.56 ATV.dial :air3 @18:42:21.89 delay 3 seconds @18:42:24.95 AS.seek 0.835ml @18:42:30.30 delay 3 seconds @18:42:33.31 ATV.dial :bleach @18:42:33.70 AS.seek 0.820ml @18:42:37.01 delay 3 seconds @18:42:40.02 ATV.dial PCR @18:42:40.34 AS.reconfigure ASconfig @18:42:40.37 AS.seek empty @18:43:28.48 PCR.program :bleachProfile @18:43:28.53 PCR program should complete in < 2:45 @18:43:28.54 PCR.run 10:00 @18:46:29.77 PCR.off @18:46:29.80 AFV.select :carrierSolution @18:46:29.89 delay 2 seconds @18:46:31.90 AS.seek 1.000ml @18:47:30.56 delay 1 second @18:47:31.57 AFV.select :holdingCoil @18:47:31.66 delay 1 second @18:47:32.67 AS.reconfigure ASfast @18:47:32.70 AS.seek 0.000ml @18:48:00.44 delay 3 seconds @18:48:03.45 AFV.select :carrierSolution @18:48:03.54 delay 2 seconds @18:48:05.55 AS.reconfigure ASconfig @18:48:05.58 AS.seek 1.000ml @18:49:04.17 delay 1 second @18:49:05.18 AFV.select :holdingCoil @18:49:05.27 delay 1 second @18:49:06.28 AS.reconfigure ASfast @18:49:06.31 AS.seek 0.000ml @18:49:34.01 delay 3 seconds @18:49:37.02 AFV.select :carrierSolution @18:49:37.11 delay 2 seconds @18:49:39.12 AS.reconfigure ASconfig @18:49:39.15 AS.seek 1.000ml @18:50:37.74 delay 1 second @18:50:38.75 AFV.select :holdingCoil @18:50:38.84 delay 1 second @18:50:39.85 AS.reconfigure ASfast @18:50:39.88 AS.seek 0.000ml @18:51:07.59 delay 3 seconds @18:51:10.60 AFV.select :carrierSolution @18:51:10.69 delay 2 seconds @18:51:12.70 AS.reconfigure ASconfig @18:51:12.73 AS.seek 1.000ml @18:52:11.35 delay 1 second @18:52:12.36 AFV.select :holdingCoil @18:52:12.45 delay 1 second @18:52:13.46 AS.reconfigure ASfast @18:52:13.49 AS.seek 0.000ml @18:52:41.19 delay 3 seconds @18:52:44.20 AFV.select :carrierSolution @18:52:44.29 delay 2 seconds @18:52:46.30 AS.reconfigure ASconfig @18:52:46.33 AS.seek 1.000ml @18:53:45.49 delay 1 second @18:53:46.50 AFV.select :holdingCoil @18:53:46.59 delay 1 second @18:53:47.60 AS.reconfigure ASnorm @18:53:47.63 AS.seek 0.000ml @18:54:39.09 Setting up PCR for IPCem -- writing /var/log/esp/IPCem14mar0718h170ml.pcr @18:54:39.10 Building PCR Slug from ASV with MM3 mix, PP5 primer @18:54:39.11 Priming with carrier @18:54:39.12 ATV.dial ARV @18:54:39.70 ARV.dial :carrier @18:54:39.99 AS.seek 0.500ml @18:55:05.91 AFV.select :carrierSolution @18:55:06.02 delay 2 seconds @18:55:08.03 AS.reconfigure ASconfig @18:55:08.07 AS.seek 1.000ml @18:55:37.51 delay 1 second @18:55:38.52 AFV.select :holdingCoil @18:55:38.61 delay 1 second @18:55:39.62 ATV.dial :waste @18:55:40.88 AS.reconfigure ASnorm @18:55:40.91 AS.seek empty @18:56:32.36 AFV.select :carrierSolution @18:56:32.46 delay 2 seconds @18:56:34.47 AS.reconfigure ASconfig @18:56:34.51 AS.seek 0.500ml @18:57:04.00 delay 1 second @18:57:05.01 AFV.select :holdingCoil @18:57:05.10 Building Slug @18:57:05.11 ATV.dial :ARV @18:57:06.36 delay 2 seconds @18:57:08.37 ARV.dial :air1 @18:57:08.70 delay 2 seconds @18:57:10.76 AS.reconfigure ASslow2 @18:57:10.79 AS.seek 0.506ml @18:57:17.11 delay 2 seconds @18:57:19.12 ARV.dial :MM3 @18:57:19.98 delay 2 seconds @18:57:21.99 Adding MM3 @18:57:22.04 AS.seek 0.524ml @18:57:40.79 delay 25 seconds @18:58:05.80 ARV.dial :PP5 @18:58:06.23 Adding PP5 @18:58:06.24 delay 2 seconds @18:58:08.30 AS.seek 0.530ml @18:58:14.49 delay 10 seconds @18:58:24.50 ARV.dial :air3 @18:58:24.82 adding ARV air @18:58:24.83 delay 2 seconds @18:58:26.89 AS.seek 0.536ml @18:58:33.13 delay 5 seconds @18:58:38.14 ARV.dial :carrier @18:58:39.17 delay 7 seconds @18:58:46.23 AS.seek 0.594ml @18:59:45.58 delay 5 seconds @18:59:50.59 ATV.dial :ASV @18:59:50.99 delay 2 seconds @18:59:53.05 AS.seek 0.600ml @18:59:59.33 delay 4 seconds @19:00:03.34 ATV.dial ARV @19:00:03.66 delay 2 seconds @19:00:05.67 ARV.dial :carrier @19:00:05.68 delay 2 seconds @19:00:07.73 AS.seek 0.650ml @19:00:58.91 delay 4 seconds @19:01:02.92 ATV.dial PCR @19:01:03.43 ASV.dial :waste1 @19:01:03.44 ARV.dial :air1 @19:01:03.77 Positioning Slug by 0.324ml @19:01:03.83 AS.seek 0.326ml @19:06:34.74 delay 5 seconds @19:06:39.75 ATV.dial ARV @19:06:40.27 ASV.dial :PCRlock @19:06:40.75 delay 5 seconds @19:06:45.76 Running IPCem ProfileEM59C thermocycling @19:06:45.77 PCR.program ProfileEM59C @19:06:45.88 ProfileEM59C should complete in < 2:11:38 @19:06:45.89 PCR.run 2:49:32.5 @21:02:41.47 PCR.off @21:02:42.01 PCR for IPCem completed -- closed /var/log/esp/IPCem14mar0718h170ml.pcr @21:02:42.02 Full MFB Decontamination @21:02:42.03 Decon extraction column and decon AIV @21:02:42.05 SPE.hold 2:46:40,55.0C @21:02:42.06 ASV.dial :SPEdelivery @21:02:42.74 delay 20 seconds @21:03:02.75 AFV.select :carrierSolution @21:03:02.84 delay 2 seconds @21:03:04.85 AS.reconfigure ASconfig @21:03:04.88 AS.seek 0.500ml @21:03:15.29 delay 1 second @21:03:16.30 AFV.select :holdingCoil @21:03:16.39 ATV.dial :air1 @21:03:17.93 AS.reconfigure ASslow1 @21:03:17.96 AS.seek 0.520ml @21:03:22.30 ATV.dial :ASV @21:03:23.58 ASV.dial :SPErecovery @21:03:24.13 AS.seek 0.620ml @21:03:44.77 ATV.dial :waste @21:03:46.22 AS.reconfigure ASnorm @21:03:46.25 AS.seek empty @21:04:18.29 AFV.select :holdingCoil @21:04:18.30 ATV.dial :waste @21:04:18.31 AS.reconfigure ASconfig @21:04:18.34 AS.seek 0.000ml @21:04:18.61 AFV.select :carrierSolution @21:04:18.71 delay 2 seconds @21:04:20.72 AS.seek 0.500ml @21:04:50.14 delay 1 second @21:04:51.15 AFV.select :holdingCoil @21:04:51.24 ATV.dial :air3 @21:04:51.80 AS.reconfigure ASslow1 @21:04:51.83 AS.seek 0.520ml @21:04:56.19 ATV.dial :air3 @21:04:56.25 AS.seek 0.530ml @21:04:58.49 delay 2 seconds @21:05:00.50 ATV.dial :bleach @21:05:00.88 AS.seek 0.710ml @21:05:37.85 delay 3 seconds @21:05:40.86 ATV.dial :air3 @21:05:41.21 delay 3 seconds @21:05:44.27 AS.seek 0.735ml @21:05:49.61 delay 3 seconds @21:05:52.62 ATV.dial :bleach @21:05:53.00 AS.seek 0.720ml @21:05:56.31 delay 3 seconds @21:05:59.32 ATV.dial ASV @21:06:00.19 ASV.dial :SPErecovery @21:06:00.25 AS.seek 0.365ml @21:07:12.95 ATV.dial :waste @21:07:14.48 AS.reconfigure ASnorm @21:07:14.51 AS.seek empty @21:07:33.55 ATV.dial :air3 @21:07:34.13 AS.reconfigure ASslow1 @21:07:34.16 AS.seek 0.009ml @21:07:36.50 delay 2 seconds @21:07:38.51 ATV.dial :bleach @21:07:38.99 AS.seek 0.579ml @21:09:35.51 delay 3 seconds @21:09:38.52 ATV.dial :air3 @21:09:38.85 delay 3 seconds @21:09:41.91 AS.seek 0.604ml @21:09:47.24 delay 3 seconds @21:09:50.25 ATV.dial :bleach @21:09:50.64 AS.seek 0.589ml @21:09:53.95 delay 3 seconds @21:09:56.96 ATV.dial :ASV @21:09:57.84 ASV.dial :wasteR @21:09:58.60 AS.seek 0.000ml @21:11:59.04 delay 1:00 @21:12:59.05 ATV.dial ASV @21:12:59.06 AFV.select :carrierSolution @21:12:59.15 delay 2 seconds @21:13:01.16 AS.reconfigure ASconfig @21:13:01.19 AS.seek 1.000ml @21:13:59.80 delay 1 second @21:14:00.81 AFV.select :holdingCoil @21:14:00.90 delay 1 second @21:14:01.91 ASV.dial :wasteR @21:14:01.92 AS.reconfigure ASslow1 @21:14:01.95 AS.seek 0.000ml @21:17:26.23 delay 10 seconds @21:17:36.24 ATV.dial :air3 @21:17:37.45 AS.seek 0.010ml @21:17:39.76 delay 2 seconds @21:17:41.77 ATV.dial :bleach @21:17:42.26 AS.seek 0.580ml @21:19:38.74 delay 3 seconds @21:19:41.75 ATV.dial :air3 @21:19:42.08 delay 3 seconds @21:19:45.14 AS.seek 0.605ml @21:19:50.45 delay 3 seconds @21:19:53.46 ATV.dial :bleach @21:19:54.44 AS.seek 0.590ml @21:19:57.73 delay 3 seconds @21:20:00.74 ATV.dial ASV @21:20:01.70 ASV.dial :SPEdelivery @21:20:02.40 AS.seek 0.000ml @21:22:03.00 delay 1:00 @21:23:03.01 AFV.select :carrierSolution @21:23:03.11 delay 2 seconds @21:23:05.12 AS.reconfigure ASconfig @21:23:05.16 AS.seek 1.000ml @21:24:03.80 delay 1 second @21:24:04.81 AFV.select :holdingCoil @21:24:04.90 delay 1 second @21:24:05.91 ATV.dial ASV @21:24:05.92 ASV.dial :SPEdelivery @21:24:05.93 AS.reconfigure ASslow1 @21:24:05.96 AS.seek 0.000ml @21:27:30.22 delay 10 seconds @21:27:40.23 ATV.dial :air2 @21:27:40.96 AS.reconfigure ASslow0 @21:27:40.99 AS.seek 0.800ml @21:29:02.92 delay 3 seconds @21:29:05.93 ATV.dial :ASV @21:29:06.65 ASV.dial :SPEdelivery @21:29:06.67 AS.reconfigure ASslow1 @21:29:06.70 AS.seek 0.030ml @21:31:43.99 delay 20 seconds @21:32:04.00 ATV.dial :waste,via => air3 @21:32:05.44 AS.reconfigure ASconfig @21:32:05.47 AS.seek empty @21:32:07.55 SPE.coast @21:32:07.63 Removing Bleach from Mixing Coil @21:32:07.64 AFV.select :carrierSolution @21:32:07.74 delay 2 seconds @21:32:09.75 AS.seek 0.500ml @21:32:39.23 delay 1 second @21:32:40.24 AFV.select :holdingCoil @21:32:40.33 ATV.dial :ASV @21:32:41.76 ASV.dial :SPErecovery @21:32:42.34 AS.reconfigure ASslow1 @21:32:42.38 AS.seek 0.730ml @21:33:29.57 ASV.dial :wasteD @21:33:30.08 AS.reconfigure ASnorm @21:33:30.11 AS.seek 0.000ml @21:34:07.71 AFV.select :carrierSolution @21:34:07.81 delay 2 seconds @21:34:09.82 AS.reconfigure ASconfig @21:34:09.85 AS.seek 0.500ml @21:34:39.30 delay 1 second @21:34:40.31 AFV.select :holdingCoil @21:34:40.40 ATV.dial ASV @21:34:40.41 ASV.dial :SPErecovery @21:34:41.12 AS.reconfigure ASslow0 @21:34:41.15 AS.seek 0.300ml @21:35:01.87 ATV.dial :air2 @21:35:02.63 AS.reconfigure ASslow1 @21:35:02.67 AS.seek 0.320ml @21:35:07.01 ATV.dial ASV @21:35:07.87 AS.reconfigure ASslow0 @21:35:07.90 AS.seek 0.570ml @21:35:33.68 ASV.dial :wasteD @21:35:34.35 AS.reconfigure ASnorm @21:35:34.38 AS.seek 0.000ml @21:36:03.85 AFV.select :carrierSolution @21:36:03.95 delay 2 seconds @21:36:05.96 AS.reconfigure ASconfig @21:36:05.99 AS.seek 0.500ml @21:36:35.43 delay 1 second @21:36:36.44 AFV.select :holdingCoil @21:36:36.53 ATV.dial ASV @21:36:36.54 ASV.dial :SPErecovery @21:36:37.24 AS.reconfigure ASslow0 @21:36:37.28 AS.seek 0.300ml @21:36:57.99 ATV.dial :air2 @21:36:58.75 AS.reconfigure ASslow1 @21:36:58.78 AS.seek 0.320ml @21:37:03.13 ATV.dial ASV @21:37:03.90 AS.reconfigure ASslow0 @21:37:03.93 AS.seek 0.570ml @21:37:29.71 ASV.dial :wasteD @21:37:30.64 AS.reconfigure ASnorm @21:37:30.67 AS.seek 0.000ml @21:38:00.12 ATV.dial :air2 @21:38:00.94 AS.reconfigure ASslow0 @21:38:00.97 AS.seek 0.300ml @21:38:31.93 ATV.dial :ASV @21:38:32.72 ASV.dial :wasteD @21:38:32.74 AS.reconfigure ASconfig @21:38:32.77 AS.seek 0.050ml @21:38:47.63 ATV.dial :waste @21:38:49.08 AS.seek 0.000ml @21:38:52.27 ASV.dial :SPEdelivery @21:38:53.11 deconLysateINT @21:38:53.12 ATV.dial :air3 @21:38:53.67 AS.reconfigure ASslow1 @21:38:53.71 AS.seek 0.010ml @21:38:56.11 delay 2 seconds @21:38:58.12 ATV.dial :bleach @21:38:58.59 AS.seek 0.960ml @21:42:12.59 delay 3 seconds @21:42:15.60 ATV.dial :air3 @21:42:15.92 delay 3 seconds @21:42:18.98 AS.seek 0.985ml @21:42:24.31 delay 3 seconds @21:42:27.32 ATV.dial :bleach @21:42:27.70 AS.seek 0.970ml @21:42:31.01 delay 3 seconds @21:42:34.02 ATV.dial :AIV @21:42:35.08 AIV.dial :coilToMFB @21:42:35.09 AWV.select :waste @21:42:35.10 AFV.select :carrierSolution @21:42:35.19 delay 2 seconds @21:42:37.20 AS.reconfigure ASconfig @21:42:37.23 AS.seek 1.000ml @21:42:39.34 delay 1 second @21:42:40.35 AFV.select :holdingCoil @21:42:40.44 delay 1 second @21:42:41.45 AS.reconfigure ASslow0 @21:42:41.48 AS.seek empty @21:44:23.87 AFV.select :carrierSolution @21:44:23.96 delay 2 seconds @21:44:25.97 AS.reconfigure ASconfig @21:44:26.00 AS.seek 1.000ml @21:45:24.68 delay 1 second @21:45:26.25 AFV.select :holdingCoil @21:45:26.34 delay 1 second @21:45:27.35 AS.reconfigure ASslow0 @21:45:27.38 AS.seek empty @21:47:09.90 AFV.select :carrierSolution @21:47:10.00 delay 2 seconds @21:47:12.01 AS.reconfigure ASconfig @21:47:12.05 AS.seek 1.000ml @21:48:10.67 delay 1 second @21:48:11.68 AFV.select :holdingCoil @21:48:11.77 delay 1 second @21:48:12.78 AS.reconfigure ASslow0 @21:48:12.81 AS.seek empty @21:49:55.20 ATV.dial :air2 @21:49:55.88 AS.reconfigure ASslow1 @21:49:55.91 AS.seek 0.009ml @21:49:58.26 AS.reconfigure ASslow0 @21:49:58.30 AS.seek 1.000ml @21:51:39.62 ATV.dial AIV @21:51:40.15 AS.seek 0.020ml @21:53:20.44 ATV.dial :waste @21:53:21.73 AS.seek 0.000ml @21:53:24.08 AWV.select :air @21:53:24.23 Cleaning PCR @21:53:24.24 AFV.select :carrierSolution @21:53:24.33 delay 2 seconds @21:53:26.34 AS.reconfigure ASconfig @21:53:26.37 AS.seek 0.500ml @21:53:55.81 delay 1 second @21:53:56.82 AFV.select :holdingCoil @21:53:56.91 ASV.dial :waste1 @21:53:57.24 ATV.dial :air3 @21:53:57.80 AS.reconfigure ASslow1 @21:53:57.83 AS.seek 0.510ml @21:54:00.14 delay 2 seconds @21:54:02.15 ATV.dial :bleach @21:54:02.52 AS.seek 0.810ml @21:55:03.97 delay 3 seconds @21:55:06.98 ATV.dial :air3 @21:55:07.33 delay 3 seconds @21:55:10.39 AS.seek 0.835ml @21:55:15.72 delay 3 seconds @21:55:18.73 ATV.dial :bleach @21:55:19.13 AS.seek 0.820ml @21:55:22.44 delay 3 seconds @21:55:25.45 ARV.dial :waste @21:55:26.06 delay 3 seconds @21:55:29.07 ATV.dial :ARV @21:55:29.89 AS.reconfigure ASconfig @21:55:29.92 AS.seek 0.470ml @21:55:50.61 AFV.select :carrierSolution @21:55:50.70 delay 2 seconds @21:55:52.71 AS.seek 1.000ml @21:56:23.95 delay 1 second @21:56:24.96 AFV.select :holdingCoil @21:56:25.05 delay 1 second @21:56:26.06 AS.reconfigure ASfast @21:56:26.09 AS.seek 0.000ml @21:56:53.82 AFV.select :carrierSolution @21:56:53.91 delay 2 seconds @21:56:55.92 AS.reconfigure ASconfig @21:56:55.95 AS.seek 1.000ml @21:57:54.57 delay 1 second @21:57:55.58 AFV.select :holdingCoil @21:57:55.67 delay 1 second @21:57:56.68 AS.reconfigure ASfast @21:57:56.71 AS.seek 0.000ml @21:58:24.45 AFV.select :carrierSolution @21:58:24.55 delay 2 seconds @21:58:26.56 AS.reconfigure ASconfig @21:58:26.59 AS.seek 1.000ml @21:59:25.21 delay 1 second @21:59:26.22 AFV.select :holdingCoil @21:59:26.31 delay 1 second @21:59:27.32 AS.reconfigure ASfast @21:59:27.35 AS.seek 0.000ml @21:59:55.08 ARV.dial between carrier and air1 @21:59:55.49 ATV.dial :air3 @21:59:56.42 AS.reconfigure ASslow1 @21:59:56.46 AS.seek 0.010ml @21:59:58.80 delay 2 seconds @22:00:00.81 ATV.dial :bleach @22:00:01.20 AS.seek 0.810ml @22:02:44.61 delay 3 seconds @22:02:47.62 ATV.dial :air3 @22:02:47.97 delay 3 seconds @22:02:51.03 AS.seek 0.835ml @22:02:56.36 delay 3 seconds @22:02:59.37 ATV.dial :bleach @22:02:59.86 AS.seek 0.820ml @22:03:03.33 delay 3 seconds @22:03:06.34 ATV.dial PCR @22:03:06.70 AS.reconfigure ASconfig @22:03:06.73 AS.seek empty @22:03:54.83 PCR.program :bleachProfile @22:03:54.88 PCR program should complete in < 2:45 @22:03:54.89 PCR.run 10:00 @22:06:59.12 PCR.off @22:06:59.15 AFV.select :carrierSolution @22:06:59.24 delay 2 seconds @22:07:01.25 AS.seek 1.000ml @22:07:59.91 delay 1 second @22:08:00.92 AFV.select :holdingCoil @22:08:01.01 delay 1 second @22:08:02.02 AS.reconfigure ASfast @22:08:02.05 AS.seek 0.000ml @22:08:29.76 delay 3 seconds @22:08:32.77 AFV.select :carrierSolution @22:08:32.86 delay 2 seconds @22:08:34.87 AS.reconfigure ASconfig @22:08:34.90 AS.seek 1.000ml @22:09:33.47 delay 1 second @22:09:34.48 AFV.select :holdingCoil @22:09:34.61 delay 1 second @22:09:35.62 AS.reconfigure ASfast @22:09:35.65 AS.seek 0.000ml @22:10:03.39 delay 3 seconds @22:10:06.40 AFV.select :carrierSolution @22:10:06.49 delay 2 seconds @22:10:08.50 AS.reconfigure ASconfig @22:10:08.54 AS.seek 1.000ml @22:11:07.67 delay 1 second @22:11:08.68 AFV.select :holdingCoil @22:11:08.77 delay 1 second @22:11:09.78 AS.reconfigure ASfast @22:11:09.81 AS.seek 0.000ml @22:11:37.54 delay 3 seconds @22:11:40.55 AFV.select :carrierSolution @22:11:40.64 delay 2 seconds @22:11:42.65 AS.reconfigure ASconfig @22:11:42.68 AS.seek 1.000ml @22:12:41.29 delay 1 second @22:12:42.30 AFV.select :holdingCoil @22:12:42.39 delay 1 second @22:12:43.40 AS.reconfigure ASfast @22:12:43.44 AS.seek 0.000ml @22:13:11.19 delay 3 seconds @22:13:14.20 AFV.select :carrierSolution @22:13:14.29 delay 2 seconds @22:13:16.30 AS.reconfigure ASconfig @22:13:16.33 AS.seek 1.000ml @22:14:14.96 delay 1 second @22:14:15.97 AFV.select :holdingCoil @22:14:16.06 delay 1 second @22:14:17.07 AS.reconfigure ASnorm @22:14:17.10 AS.seek 0.000ml @22:15:08.48 AFV.select :carrierSolution @22:15:08.57 delay 2 seconds @22:15:10.58 AS.reconfigure ASconfig @22:15:10.61 AS.seek 1.000ml @22:16:09.22 delay 1 second @22:16:10.23 AFV.select :holdingCoil @22:16:10.32 delay 1 second @22:16:11.33 ATV.dial :waste @22:16:12.25 AS.reconfigure ASnorm @22:16:12.28 AS.seek 0.500ml @22:16:38.20 ATV.dial :waste2 @22:16:39.29 AS.seek 0.000ml @22:17:05.21
Cleaning PCR @22:17:05.22 AFV.select :carrierSolution @22:17:05.31 delay 2 seconds @22:17:07.32 AS.reconfigure ASconfig @22:17:07.35 AS.seek 0.500ml @22:17:36.83 delay 1 second @22:17:37.84 AFV.select :holdingCoil @22:17:37.93 ASV.dial :waste1 @22:17:37.94 ATV.dial :air3 @22:17:39.50 AS.reconfigure ASslow1 @22:17:39.53 AS.seek 0.510ml @22:17:41.81 delay 2 seconds @22:17:43.82 ATV.dial :bleach @22:17:44.31 AS.seek 0.810ml @22:18:45.73 delay 3 seconds @22:18:48.74 ATV.dial :air3 @22:18:49.07 delay 3 seconds @22:18:52.13 AS.seek 0.835ml @22:18:57.45 delay 3 seconds @22:19:00.47 ATV.dial :bleach @22:19:00.86 AS.seek 0.820ml @22:19:04.17 delay 3 seconds @22:19:07.19 ARV.dial :waste @22:19:07.60 delay 3 seconds @22:19:10.61 ATV.dial :ARV @22:19:11.33 AS.reconfigure ASconfig @22:19:11.37 AS.seek 0.470ml @22:19:32.06 AFV.select :carrierSolution @22:19:32.15 delay 2 seconds @22:19:34.16 AS.seek 1.000ml @22:20:05.34 delay 1 second @22:20:06.35 AFV.select :holdingCoil @22:20:06.44 delay 1 second @22:20:07.45 AS.reconfigure ASfast @22:20:07.48 AS.seek 0.000ml @22:20:35.21 AFV.select :carrierSolution @22:20:35.30 delay 2 seconds @22:20:37.31 AS.reconfigure ASconfig @22:20:37.34 AS.seek 1.000ml @22:21:35.94 delay 1 second @22:21:36.96 AFV.select :holdingCoil @22:21:37.05 delay 1 second @22:21:38.06 AS.reconfigure ASfast @22:21:38.09 AS.seek 0.000ml @22:22:05.83 AFV.select :carrierSolution @22:22:05.92 delay 2 seconds @22:22:07.93 AS.reconfigure ASconfig @22:22:07.96 AS.seek 1.000ml @22:23:06.55 delay 1 second @22:23:07.57 AFV.select :holdingCoil @22:23:07.66 delay 1 second @22:23:08.67 AS.reconfigure ASfast @22:23:08.70 AS.seek 0.000ml @22:23:36.44 ARV.dial between carrier and air1 @22:23:36.86 ATV.dial :air3 @22:23:37.80 AS.reconfigure ASslow1 @22:23:37.83 AS.seek 0.010ml @22:23:40.21 delay 2 seconds @22:23:42.22 ATV.dial :bleach @22:23:42.61 AS.seek 0.810ml @22:26:26.04 delay 3 seconds @22:26:29.05 ATV.dial :air3 @22:26:29.40 delay 3 seconds @22:26:32.46 AS.seek 0.835ml @22:26:37.87 delay 3 seconds @22:26:40.89 ATV.dial :bleach @22:26:41.35 AS.seek 0.820ml @22:26:44.65 delay 3 seconds @22:26:47.66 ATV.dial PCR @22:26:48.00 AS.reconfigure ASconfig @22:26:48.03 AS.seek empty @22:27:36.15 PCR.program :bleachProfile @22:27:36.21 PCR program should complete in < 2:45 @22:27:36.22 PCR.run 10:00 @22:30:40.45 PCR.off @22:30:40.48 AFV.select :carrierSolution @22:30:40.57 delay 2 seconds @22:30:42.58 AS.seek 1.000ml @22:31:41.23 delay 1 second @22:31:42.25 AFV.select :holdingCoil @22:31:42.34 delay 1 second @22:31:43.35 AS.reconfigure ASfast @22:31:43.38 AS.seek 0.000ml @22:32:11.11 delay 3 seconds @22:32:14.13 AFV.select :carrierSolution @22:32:14.22 delay 2 seconds @22:32:16.71 AS.reconfigure ASconfig @22:32:16.74 AS.seek 1.000ml @22:33:15.34 delay 1 second @22:33:16.36 AFV.select :holdingCoil @22:33:16.45 delay 1 second @22:33:17.46 AS.reconfigure ASfast @22:33:17.50 AS.seek 0.000ml @22:33:45.23 delay 3 seconds @22:33:48.24 AFV.select :carrierSolution @22:33:48.33 delay 2 seconds @22:33:50.34 AS.reconfigure ASconfig @22:33:50.38 AS.seek 1.000ml @22:34:48.99 delay 1 second @22:34:50.01 AFV.select :holdingCoil @22:34:50.10 delay 1 second @22:34:51.11 AS.reconfigure ASfast @22:34:51.14 AS.seek 0.000ml @22:35:18.90 delay 3 seconds @22:35:21.91 AFV.select :carrierSolution @22:35:22.00 delay 2 seconds @22:35:24.01 AS.reconfigure ASconfig @22:35:24.04 AS.seek 1.000ml @22:36:22.64 delay 1 second @22:36:23.66 AFV.select :holdingCoil @22:36:23.75 delay 1 second @22:36:24.76 AS.reconfigure ASfast @22:36:24.80 AS.seek 0.000ml @22:36:52.53 delay 3 seconds @22:36:55.54 AFV.select :carrierSolution @22:36:55.63 delay 2 seconds @22:36:57.64 AS.reconfigure ASconfig @22:36:57.67 AS.seek 1.000ml @22:37:56.30 delay 1 second @22:37:57.31 AFV.select :holdingCoil @22:37:57.40 delay 1 second @22:37:58.41 AS.reconfigure ASnorm @22:37:58.45 AS.seek 0.000ml @22:38:49.89 Completed {wqPCR} begun 08:01:03 @22:38:49.92 Asleep til 08:01PST08-Mar-14 @22:38:49.93 AS.stop @22:38:49.95
Sleeping until 08:01PST08-Mar-14 @22:38:49.97 GoodNews.email "Then, in tube 2: wqPCR\n/home/esp/esp2local/mission/14mar05sccwrpDeploy.rb:64",Subject => "Asleep until 08:01PST08-Mar-14" @22:38:51.74 CC.stop @22:38:51.77 CS.stop @22:38:51.79 PC.stop @22:38:51.82 PS.stop @22:38:51.85 Forearm.stop @22:38:51.88 Elbow.stop @22:38:51.91 SS.stop @22:38:51.98 Sleepy.power :raw,:OFF @22:38:51.99 Sleepy.power :dwarf,:OFF Shutdown @06:49:22.08PST08-Mar-14 Session Begun @06:49:27.74 -> progress @06:49:39.60 -> exit @06:49:39.61 Session Ended @08:01:03.49
Powering up Dwarves @08:01:08.40 Sleepy.power :raw,:ON @08:01:08.87 SPE.configure SPEconfig @08:01:08.89 AS.configure ASconfig @08:01:08.92 CC.configure CCconfig @08:01:08.95 CS.configure CSconfig @08:01:08.98 PC.configure PCconfig @08:01:09.01 PS.configure PSconfig @08:01:09.04 Forearm.configure LooseForearm @08:01:09.07 Elbow.configure LooseElbow @08:01:09.10 SC.configure SCloose @08:01:09.13 SE.configure SEconfig @08:01:09.16 SS.configure SSconfig @08:01:09.19 CH.configure CHconfig @08:01:09.21 PH.configure PHconfig @08:01:09.24 Valve.reset @08:01:09.90 PCR.off @08:01:09.93 PCR.pidtemp= 50,0.1,0,300,25,25 @08:01:09.96 PCR.caltemp= 6.6661,0.0344,0.0 @08:01:10.08 Intake.select :close @08:01:10.09 Exhaust.select :close @08:01:10.10 CTV.dial :air @08:01:10.14 PSV.dial :air @08:01:10.18
PC.reconfigure PCconfig @08:01:10.22 PC.home.seek open @08:01:10.24 CSV.dial :waste1 @08:01:10.60 PS.reconfigure PSconfig @08:01:10.64 PS.home.seek 0.000ml @08:01:10.70 SSV.dial :spit,avoiding => exhaust @08:01:11.49 CS.reconfigure CSfast @08:01:11.52 SS.reconfigure SSconfig @08:01:11.61 SS.home.seek 0.00ml @08:01:11.63 CS.home.seek 0.000ml @08:01:12.18 CC.reconfigure CCconfig @08:01:12.21 CC.home.seek open @08:01:21.51 PS.coast @08:01:21.59 PC.seek open @08:01:21.63
CC.seek open @08:01:21.78 Forearm.reconfigure LooseForearm @08:01:21.81 Forearm.home.seek retract @08:01:22.54 Hand.grip @08:01:22.57
Elbow.jog -1500 @08:01:23.02 Elbow.reconfigure LooseElbow @08:01:23.06 Elbow.home.seek clear @08:01:27.80 SE.reconfigure SEconfig @08:01:27.83 SE.home.seek down @08:01:28.32 SC.reconfigure SCsloppy @08:01:28.36 SC.home.seek initial @08:01:30.61 Forearm.seek retract @08:01:30.67 Elbow.seek hammer @08:01:31.85 Forearm.reconfigure TightForearm @08:01:31.90 Forearm.seek 2 @08:01:32.99 Forearm.seek retract @08:01:34.11 Forearm.reconfigure LooseForearm @08:01:34.53PST08-Mar-14 ===> STARTED wqPCR (phase #5) <=== @08:01:34.54 Starting initialPurge @08:01:34.55 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:01:34.60 Hand.release @08:01:35.46 Elbow.reconfigure TightElbow @08:01:35.49 Elbow.seek FlushPuck::Garage @08:01:37.26 Elbow.reconfigure LooseElbow @08:01:37.34 Forearm.seek garage @08:01:38.29 Hand.grip @08:01:39.09 Forearm.seek retract @08:01:40.07 Elbow.reconfigure TightElbow @08:01:40.11 Elbow.seek CC @08:01:42.87 Elbow.reconfigure LooseElbow @08:01:42.96 Forearm.reconfigure TightForearm @08:01:42.99 Forearm.seek CC @08:01:44.10 CC.seek closed @08:01:47.87 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.90 @08:01:47.92 Hand.release @08:01:48.66 Forearm.reconfigure LooseForearm @08:01:48.69 Forearm.seek retract @08:01:49.64 Flushing the Collection Valve::Manifold @08:01:49.73 CS Pulling 5ml of flush @08:01:49.74 CSV.dial CSR @08:01:51.01 CSR.select :flush @08:01:51.11 CS.reconfigure CSconfig @08:01:51.15 CS.seek 5.000ml @08:02:04.54 delay 5 seconds @08:02:09.55 CSR.select :air @08:02:09.70 CS.reconfigure CSfast @08:02:09.73 CS.seek 7.500ml @08:02:16.38 CSV.dial :waste @08:02:17.63 CS.reconfigure CSconfig @08:02:17.67 CS.seek 0.000ml @08:02:37.44 Purging external Intake @08:02:37.53 CSV.dial :flush @08:02:37.99 CS.reconfigure CSfast @08:02:38.02 CS.seek 10.000ml @08:03:01.79 delay 5 seconds @08:03:06.80 CSV.dial CBV @08:03:06.82
CBV.dial CSV @08:03:07.66 CTV.dial :intake @08:03:08.40 Intake.select :open @08:03:08.49 CS.seek 0.000ml @08:03:32.97 Intake.select :close @08:03:33.06 CTV.dial :air @08:03:33.82 Flushing upper manifold @08:03:33.91 CSV.dial :flush @08:03:34.70 CS.seek 5.000ml @08:03:47.10 delay 5 seconds @08:03:52.12 CSV.dial :air @08:03:52.58 CS.seek 7.000ml @08:03:58.00 CSV.dial CBV @08:03:58.02
CBV.dial CSV @08:03:59.08 CTV.dial :air @08:03:59.10 CS.seek 0.000ml @08:04:15.94 delay 7 seconds @08:04:22.96 CS.seek 3.000ml @08:04:30.72 CSV.dial :waste @08:04:31.23 CS.seek 0.000ml @08:04:39.05 CSV.dial :flush @08:04:39.48 CS.seek 3.000ml @08:04:47.22 delay 5 seconds @08:04:52.23 CSV.dial :air @08:04:52.71 CS.seek 5.000ml @08:04:58.22 CSV.dial :air @08:04:58.23 CS.seek 0.000ml @08:05:10.53 delay 7 seconds @08:05:17.55 CS.seek 3.000ml @08:05:25.28 CSV.dial :waste @08:05:26.07 CS.seek 0.000ml @08:05:33.84 Garaging FlushPuck in Collection Clamp @08:05:33.90 Elbow.reconfigure TightElbow @08:05:33.93 Elbow.seek CC @08:05:34.17 Elbow.reconfigure LooseElbow @08:05:34.25 Forearm.seek CC @08:05:35.21 CC.seek sealed @08:05:36.16 Hand.grip @08:05:36.86 CC.seek open @08:05:40.15 Forearm.seek retract @08:05:41.13 Elbow.reconfigure TightElbow @08:05:41.17 Elbow.seek garage @08:05:44.54 Elbow.reconfigure LooseElbow @08:05:44.63 Forearm.reconfigure TightForearm @08:05:44.66 Forearm.seek garage @08:05:45.83 Hand.release @08:05:46.66 Forearm.reconfigure LooseForearm @08:05:46.69 Forearm.seek retract @08:05:47.77 Starting mfb @08:05:47.78 pcrBAC sampling at most 1000ml, wcr at most 1000ml @08:05:47.80 Loading sh1 puck from tube 2 into Collection Clamp @08:05:47.83 BACfans.email "Can@08:01:34, 14.6C, 64% humidity, 14.2psia, 13.739V, 0.567A, 0.567A avg, 7.79W\nCTD data unavailable\nISUS data unavailable",Subject => "pcrBAC sampling at most 1000ml, wcr at most 1000ml" @08:05:49.41 Readying the MFB @08:05:49.42 Homing MFB Syringe AFV.select :holdingCoil @08:05:49.67 ATV.dial :air2 @08:05:49.72
SC.reconfigure SCloose @08:05:49.90 Elbow.reconfigure TightElbow @08:05:49.94 Elbow.seek 2 @08:05:50.97 AS.reconfigure ASconfig @08:05:51.00 AS.home.seek home @08:05:51.88
Forearm.reconfigure TightForearm @08:05:51.92 Forearm.seek 2 + 800 counts @08:05:53.04 SE.reconfigure SEpick @08:05:53.09 SE.seek beamBreak @08:05:55.16 SE.configure SEconfig @08:05:55.29 Elbow.seek 2 @08:05:55.56 Forearm.seek 2 + 300 counts @08:05:55.86 Forearm.reconfigure LooseForearm @08:05:55.90 Hand.grip @08:05:56.64 SE.seek down @08:05:56.77
Forearm.seek retract @08:05:57.76 Elbow.seek CC @08:05:58.50 SE.coast @08:05:58.54 AFV.select :carrierSolution @08:05:58.63 delay 2 seconds @08:05:59.32
Elbow.reconfigure LooseElbow @08:05:59.40 Forearm.reconfigure TightForearm @08:05:59.43 Forearm.seek CC @08:06:00.53 CC.seek closed @08:06:00.64 AS.seek 1.000ml @08:06:04.31
Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.87 @08:06:04.32 Hand.release @08:06:05.22 Forearm.reconfigure LooseForearm @08:06:05.26 Forearm.seek retract @08:06:06.21 SC.coast @08:06:06.24 collectSample 1000ml w/bubble point of 55psi Priming 7ml @08:06:06.25 Sleepy.power :sampler,:ON @08:06:06.26 Intake.select :close Exhaust.select :close @08:06:06.27 SS.hold @08:06:06.43 Calibrating pressure and temperature sensors @08:06:06.45 CTV.dial :air @08:06:06.46 SSV.dial :spit,avoiding => exhaust @08:06:06.47 SS.reconfigure SSfast @08:06:06.50 SS.seek 5.00ml @08:06:09.25 SSV.dial CBV @08:06:09.27
CBV.dial SSV @08:06:10.66 delay 3.8 seconds @08:06:14.54 SSV.dial :spit @08:06:15.90 SS.seek 0.00ml @08:06:18.55 SSV.dial CBV @08:06:19.89 CTV.dial :intake @08:06:20.66 Waiting for external sampler to finish priming... @08:06:20.67 delayUntil "08:16:06.25" @08:06:52.37 delay 1 second @08:06:53.38 AFV.select :holdingCoil @08:06:53.47 delay 1 second @08:06:54.48 ATV.dial :waste @08:06:55.91 AS.reconfigure ASnorm @08:06:55.94 AS.home.seek empty @08:07:49.08 ATV.dial :waste @08:07:49.09 ARV.dial between carrier and air1 @08:07:49.16 ASV.dial :waste1 @08:16:06.25 SSV.dial CBV @08:16:06.27
CBV.dial SSV @08:16:06.28 Allowing 55s for 7.0ml of remaining 7.0ml Intake.select :open @08:16:08.42 Intake @51.5psia, vacuum from 20.0 to 24.0psi @08:16:08.43 SS.reconfigure forSampling @08:16:08.47 SS.seek 7.00ml @08:16:14.32 Stroke completed -- waiting for pressure to equalize ... @08:16:14.96 Intake.select :close @08:16:15.09 SSV.dial :exhaust @08:16:15.86 Exhaust.select :open @08:16:15.97 SS.reconfigure SSexhale @08:16:16.01 SS.seek 0.00ml @08:16:19.94 Exhaust.select :close @08:16:20.04 Sampling 1000.0ml @08:16:20.05 Intake.select :close @08:16:20.06 Exhaust.select :close @08:16:20.07 SS.reconfigure SSconfig @08:16:20.10 SS.hold @08:16:20.25 CTV.dial :intake @08:16:20.26 SSV.dial CBV @08:16:20.28
CBV.dial SSV @08:16:21.06 Allowing 185s for 25.0ml of remaining 1000.0ml Intake.select :open @08:16:23.19 Intake @51.2psia, vacuum from 20.0 to 24.0psi @08:16:23.20 SS.reconfigure forSampling @08:16:23.24 SS.seek 25.00ml @08:16:38.11 Stroke completed -- waiting for pressure to equalize ... @08:16:38.75 Intake.select :close @08:16:38.88 SSV.dial :exhaust @08:16:39.66 Exhaust.select :open @08:16:39.76 SS.reconfigure SSexhale @08:16:39.80 SS.seek 0.00ml @08:16:48.91 Exhaust.select :close @08:16:49.00 SSV.dial CBV @08:16:49.02
CBV.dial SSV @08:16:49.80 Allowing 185s for 25.0ml of remaining 975.0ml @08:16:49.81 Intake.select :open @08:16:51.94 Intake @50.9psia, vacuum from 20.0 to 24.0psi @08:16:51.95 SS.reconfigure forSampling @08:16:51.99 SS.seek 25.00ml @08:16:58.01 Vacuum too high -- waiting up to 40s @08:16:58.28 Intake @43.2psia, vacuum from 20.0 to 24.0psi @08:16:58.29 SS.reconfigure forSampling @08:16:58.32 SS.seek 25.00ml @08:17:08.11 Stroke completed -- waiting for pressure to equalize ... @08:17:08.75 Intake.select :close @08:17:08.88 SSV.dial :exhaust @08:17:09.64 Exhaust.select :open @08:17:09.73 SS.reconfigure SSexhale @08:17:09.77 SS.seek 0.00ml @08:17:18.89 Exhaust.select :close @08:17:18.98 SSV.dial CBV @08:17:19.00
CBV.dial SSV @08:17:19.78 Allowing 185s for 25.0ml of remaining 950.0ml @08:17:19.79 Intake.select :open @08:17:21.92 Intake @50.0psia, vacuum from 20.0 to 24.0psi @08:17:21.93 SS.reconfigure forSampling @08:17:21.97 SS.seek 25.00ml @08:17:25.51 Vacuum too high -- waiting up to 40s @08:17:25.78 Intake @45.8psia, vacuum from 20.0 to 24.0psi @08:17:25.79 SS.reconfigure forSampling @08:17:25.82 SS.seek 25.00ml @08:17:28.02 Vacuum too high -- waiting up to 40s @08:17:28.28 Intake @44.7psia, vacuum from 20.0 to 24.0psi SS.reconfigure forSampling @08:17:28.32 SS.seek 25.00ml @08:17:32.26 Vacuum too high -- waiting up to 40s @08:17:32.53 Intake @46.4psia, vacuum from 20.0 to 24.0psi @08:17:32.54 SS.reconfigure forSampling @08:17:32.57 SS.seek 25.00ml @08:17:34.87 Vacuum too high -- waiting up to 40s @08:17:35.13 Intake @47.1psia, vacuum from 20.0 to 24.0psi @08:17:35.14 SS.reconfigure forSampling @08:17:35.17 SS.seek 25.00ml @08:17:38.79 Vacuum too high -- waiting up to 40s @08:17:39.05 Intake @48.0psia, vacuum from 20.0 to 24.0psi @08:17:39.06 SS.reconfigure forSampling @08:17:39.10 SS.seek 25.00ml @08:17:44.98 Vacuum too high -- waiting up to 40s @08:17:45.25 Intake @48.0psia, vacuum from 20.0 to 24.0psi @08:17:45.26 SS.reconfigure forSampling @08:17:45.29 SS.seek 25.00ml @08:17:51.03 Stroke completed -- waiting for pressure to equalize ... @08:17:51.67 Intake.select :close @08:17:51.80 SSV.dial :exhaust @08:17:52.56 Exhaust.select :open @08:17:52.66 SS.reconfigure SSexhale @08:17:52.70 SS.seek 0.00ml @08:18:01.78 Exhaust.select :close @08:18:01.87 SSV.dial CBV @08:18:01.89
CBV.dial SSV @08:18:02.67 Allowing 185s for 25.0ml of remaining 925.0ml @08:18:02.68 Intake.select :open @08:18:04.81 Intake @49.5psia, vacuum from 20.0 to 24.0psi @08:18:04.82 SS.reconfigure forSampling @08:18:04.86 SS.seek 25.00ml @08:18:13.54 Vacuum too high -- waiting up to 40s @08:18:13.80 Intake @49.1psia, vacuum from 20.0 to 24.0psi @08:18:13.81 SS.reconfigure forSampling @08:18:13.85 SS.seek 25.00ml @08:18:49.38 Vacuum too high -- waiting up to 40s @08:18:49.65 Intake @48.9psia, vacuum from 20.0 to 24.0psi @08:18:49.66 SS.reconfigure forSampling @08:18:49.70 SS.seek 25.00ml @08:19:12.07 Stroke completed -- waiting for pressure to equalize ... @08:19:12.71 Intake.select :close @08:19:12.84 SSV.dial :exhaust @08:19:13.62 Exhaust.select :open @08:19:13.73 SS.reconfigure SSexhale @08:19:13.77 SS.seek 0.00ml @08:19:22.71 Exhaust.select :close @08:19:22.80 SSV.dial CBV @08:19:22.82
CBV.dial SSV @08:19:23.60 Allowing 185s for 25.0ml of remaining 900.0ml @08:19:23.61 Intake.select :open @08:19:25.74 Intake @48.9psia, vacuum from 20.0 to 24.0psi @08:19:25.75 SS.reconfigure forSampling @08:19:25.79 SS.seek 25.00ml @08:21:15.97 Stroke completed -- waiting for pressure to equalize ... @08:21:17.19 Intake.select :close @08:21:17.32 SSV.dial :exhaust @08:21:18.10 Exhaust.select :open @08:21:18.21 SS.reconfigure SSexhale @08:21:18.25 SS.seek 0.00ml @08:21:27.36 Exhaust.select :close @08:21:27.45 SSV.dial CBV @08:21:27.47
CBV.dial SSV @08:21:28.27 Allowing 185s for 25.0ml of remaining 875.0ml @08:21:28.28 Intake.select :open @08:21:30.41 Intake @49.5psia, vacuum from 20.0 to 24.0psi @08:21:30.42 SS.reconfigure forSampling @08:21:30.46 SS.seek 25.00ml @08:22:18.78 Vacuum too high -- waiting up to 40s @08:22:19.05 Intake @48.3psia, vacuum from 20.0 to 24.0psi @08:22:19.06 SS.reconfigure forSampling @08:22:19.09 SS.seek 25.00ml @08:22:26.47 Vacuum too high -- waiting up to 40s @08:22:26.74 Intake @47.9psia, vacuum from 20.0 to 24.0psi @08:22:26.75 SS.reconfigure forSampling @08:22:26.79 SS.seek 25.00ml @08:22:34.41 Vacuum too high -- waiting up to 40s @08:22:34.67 Intake @47.7psia, vacuum from 20.0 to 24.0psi @08:22:34.68 SS.reconfigure forSampling @08:22:34.71 SS.seek 25.00ml @08:22:37.84 Vacuum too high -- waiting up to 40s @08:22:38.11 Intake @47.5psia, vacuum from 20.0 to 24.0psi @08:22:38.12 SS.reconfigure forSampling @08:22:38.15 SS.seek 25.00ml @08:22:46.41 Vacuum too high -- waiting up to 40s @08:22:46.68 Intake @47.8psia, vacuum from 20.0 to 24.0psi @08:22:46.69 SS.reconfigure forSampling @08:22:46.72 SS.seek 25.00ml @08:22:49.27 Vacuum too high -- waiting up to 40s @08:22:49.53 Intake @48.0psia, vacuum from 20.0 to 24.0psi @08:22:49.54 SS.reconfigure forSampling @08:22:49.57 SS.seek 25.00ml @08:22:51.23 Vacuum too high -- waiting up to 40s @08:22:51.49 Intake @47.7psia, vacuum from 20.0 to 24.0psi @08:22:51.50 SS.reconfigure forSampling @08:22:51.53 SS.seek 25.00ml @08:22:53.89 Vacuum too high -- waiting up to 40s @08:22:54.15 Intake @47.8psia, vacuum from 20.0 to 24.0psi @08:22:54.16 SS.reconfigure forSampling @08:22:54.19 SS.seek 25.00ml @08:22:55.73 Vacuum too high -- waiting up to 40s @08:22:55.99 Intake @47.4psia, vacuum from 20.0 to 24.0psi @08:22:56.00 SS.reconfigure forSampling @08:22:56.03 SS.seek 25.00ml @08:22:58.83 Vacuum too high -- waiting up to 40s @08:22:59.09 Intake @48.9psia, vacuum from 20.0 to 24.0psi @08:22:59.10 SS.reconfigure forSampling @08:22:59.14 SS.seek 25.00ml @08:23:00.42 Vacuum too high -- waiting up to 40s @08:23:00.68 Intake @48.2psia, vacuum from 20.0 to 24.0psi @08:23:00.69 SS.reconfigure forSampling @08:23:00.72 SS.seek 25.00ml @08:23:02.48 Vacuum too high -- waiting up to 40s @08:23:02.74 Intake @47.9psia, vacuum from 20.0 to 24.0psi @08:23:02.75 SS.reconfigure forSampling @08:23:02.78 SS.seek 25.00ml @08:23:04.27 Vacuum too high -- waiting up to 40s @08:23:04.54 SS.seek 25.00ml @08:23:06.20 Vacuum too high -- waiting up to 40s @08:23:06.46 Intake @47.7psia, vacuum from 20.0 to 24.0psi @08:23:06.47 SS.reconfigure forSampling @08:23:06.50 SS.seek 25.00ml @08:23:08.14 Vacuum too high -- waiting up to 40s @08:23:08.40 Intake @47.5psia, vacuum from 20.0 to 24.0psi @08:23:08.41 SS.reconfigure forSampling @08:23:08.44 SS.seek 25.00ml @08:23:09.80 Vacuum too high -- waiting up to 40s @08:23:10.06 SS.seek 25.00ml @08:23:11.89 Vacuum too high -- waiting up to 40s @08:23:12.14 Intake @48.3psia, vacuum from 20.0 to 24.0psi @08:23:12.15 SS.reconfigure forSampling @08:23:12.19 SS.seek 25.00ml @08:23:13.48 Vacuum too high -- waiting up to 40s @08:23:13.75 SS.seek 25.00ml @08:23:15.08 Vacuum too high -- waiting up to 40s @08:23:15.34 Intake @48.0psia, vacuum from 20.0 to 24.0psi @08:23:15.91 SS.reconfigure forSampling @08:23:15.94 SS.seek 25.00ml @08:23:18.26 Vacuum too high -- waiting up to 40s @08:23:18.52 Intake @48.4psia, vacuum from 20.0 to 24.0psi @08:23:18.53 SS.reconfigure forSampling @08:23:18.56 SS.seek 25.00ml @08:23:19.83 Vacuum too high -- waiting up to 40s @08:23:20.09 Intake @48.3psia, vacuum from 20.0 to 24.0psi @08:23:20.10 SS.reconfigure forSampling @08:23:20.14 SS.seek 25.00ml @08:23:21.67 Vacuum too high -- waiting up to 40s @08:23:21.93 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:23:21.94 SS.reconfigure forSampling @08:23:21.97 SS.seek 25.00ml @08:23:23.30 Vacuum too high -- waiting up to 40s @08:23:23.56 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:23:23.57 SS.reconfigure forSampling @08:23:23.60 SS.seek 25.00ml @08:23:24.83 Vacuum too high -- waiting up to 40s @08:23:25.09 Intake @47.9psia, vacuum from 20.0 to 24.0psi @08:23:25.10 SS.reconfigure forSampling @08:23:25.14 SS.seek 25.00ml @08:23:26.80 Vacuum too high -- waiting up to 40s @08:23:27.06 Intake @47.8psia, vacuum from 20.0 to 24.0psi @08:23:27.07 SS.reconfigure forSampling @08:23:27.10 SS.seek 25.00ml @08:23:28.33 Vacuum too high -- waiting up to 40s @08:23:28.59 Intake @47.9psia, vacuum from 20.0 to 24.0psi @08:23:28.60 SS.reconfigure forSampling @08:23:28.63 SS.seek 25.00ml @08:23:30.48 Vacuum too high -- waiting up to 40s @08:23:30.74 Intake @48.8psia, vacuum from 20.0 to 24.0psi @08:23:30.75 SS.reconfigure forSampling @08:23:30.79 SS.seek 25.00ml @08:23:32.83 Vacuum too high -- waiting up to 40s @08:23:33.09 Intake @48.1psia, vacuum from 20.0 to 24.0psi @08:23:33.10 SS.reconfigure forSampling @08:23:33.13 SS.seek 25.00ml @08:23:33.99 Stroke completed -- waiting for pressure to equalize ... @08:23:35.81 Intake.select :close @08:23:35.94 SSV.dial :exhaust @08:23:36.74 Exhaust.select :open @08:23:36.84 SS.reconfigure SSexhale @08:23:36.88 SS.seek 0.00ml @08:23:46.07 Exhaust.select :close @08:23:46.16 SSV.dial CBV @08:23:46.18
CBV.dial SSV @08:23:46.99 Allowing 185s for 25.0ml of remaining 850.0ml @08:23:47.00 Intake.select :open @08:23:49.13 Intake @48.6psia, vacuum from 20.0 to 24.0psi @08:23:49.14 SS.reconfigure forSampling @08:23:49.18 SS.seek 25.00ml @08:23:51.61 Vacuum too high -- waiting up to 40s @08:23:51.87 Intake @48.0psia, vacuum from 20.0 to 24.0psi @08:23:51.88 SS.reconfigure forSampling @08:23:51.92 SS.seek 25.00ml @08:23:53.61 Vacuum too high -- waiting up to 40s @08:23:53.97 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:23:53.98 SS.reconfigure forSampling @08:23:54.01 SS.seek 25.00ml @08:23:55.76 Vacuum too high -- waiting up to 40s @08:23:56.03 SS.seek 25.00ml @08:23:57.67 Vacuum too high -- waiting up to 40s @08:23:57.93 Intake @47.8psia, vacuum from 20.0 to 24.0psi @08:23:57.94 SS.reconfigure forSampling @08:23:57.97 SS.seek 25.00ml @08:23:59.20 Vacuum too high -- waiting up to 40s @08:23:59.46 Intake @47.5psia, vacuum from 20.0 to 24.0psi @08:23:59.47 SS.reconfigure forSampling @08:23:59.50 SS.seek 25.00ml @08:24:00.89 Vacuum too high -- waiting up to 40s @08:24:01.15 Intake @48.2psia, vacuum from 20.0 to 24.0psi @08:24:01.16 SS.reconfigure forSampling @08:24:01.19 SS.seek 25.00ml @08:24:02.76 Vacuum too high -- waiting up to 40s @08:24:03.01 Intake @48.5psia, vacuum from 20.0 to 24.0psi @08:24:03.02 SS.reconfigure forSampling @08:24:03.06 SS.seek 25.00ml @08:24:04.22 Vacuum too high -- waiting up to 40s @08:24:04.49 Intake @48.0psia, vacuum from 20.0 to 24.0psi @08:24:04.50 SS.reconfigure forSampling @08:24:04.54 SS.seek 25.00ml @08:24:05.73 Vacuum too high -- waiting up to 40s @08:24:05.99 Intake @47.9psia, vacuum from 20.0 to 24.0psi @08:24:06.00 SS.reconfigure forSampling @08:24:06.03 SS.seek 25.00ml @08:24:07.14 Vacuum too high -- waiting up to 40s @08:24:07.40 Intake @47.4psia, vacuum from 20.0 to 24.0psi @08:24:07.41 SS.reconfigure forSampling @08:24:07.44 SS.seek 25.00ml @08:24:08.67 Vacuum too high -- waiting up to 40s @08:24:08.93 Intake @47.3psia, vacuum from 20.0 to 24.0psi @08:24:08.94 SS.reconfigure forSampling @08:24:08.97 SS.seek 25.00ml @08:24:10.23 Vacuum too high -- waiting up to 40s @08:24:10.49 Intake @47.3psia, vacuum from 20.0 to 24.0psi @08:24:10.50 SS.reconfigure forSampling @08:24:11.09 SS.seek 25.00ml @08:24:13.36 Vacuum too high -- waiting up to 40s @08:24:13.62 Intake @47.7psia, vacuum from 20.0 to 24.0psi @08:24:13.63 SS.reconfigure forSampling @08:24:13.66 SS.seek 25.00ml @08:24:14.73 Vacuum too high -- waiting up to 40s @08:24:14.99 Intake @47.4psia, vacuum from 20.0 to 24.0psi @08:24:15.19 SS.reconfigure forSampling @08:24:15.22 SS.seek 25.00ml @08:24:16.79 Vacuum too high -- waiting up to 40s @08:24:17.05 Intake @47.7psia, vacuum from 20.0 to 24.0psi @08:24:17.06 SS.reconfigure forSampling @08:24:17.09 SS.seek 25.00ml @08:24:18.51 Vacuum too high -- waiting up to 40s @08:24:18.77 Intake @48.7psia, vacuum from 20.0 to 24.0psi @08:24:18.78 SS.reconfigure forSampling @08:24:18.81 SS.seek 25.00ml @08:24:19.76 Vacuum too high -- waiting up to 40s @08:24:20.02 Intake @48.2psia, vacuum from 20.0 to 24.0psi @08:24:20.03 SS.reconfigure forSampling @08:24:20.07 SS.seek 25.00ml @08:24:21.14 Vacuum too high -- waiting up to 40s @08:24:21.40 Intake @48.2psia, vacuum from 20.0 to 24.0psi @08:24:21.41 SS.reconfigure forSampling @08:24:21.44 SS.seek 25.00ml @08:24:22.54 Vacuum too high -- waiting up to 40s @08:24:22.80 Intake @47.9psia, vacuum from 20.0 to 24.0psi @08:24:22.81 SS.reconfigure forSampling @08:24:22.84 SS.seek 25.00ml @08:24:23.92 Vacuum too high -- waiting up to 40s @08:24:24.18 Intake @47.4psia, vacuum from 20.0 to 24.0psi @08:24:24.19 SS.reconfigure forSampling @08:24:24.22 SS.seek 25.00ml @08:24:25.29 Vacuum too high -- waiting up to 40s @08:24:25.55 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:24:25.56 SS.reconfigure forSampling @08:24:25.59 SS.seek 25.00ml @08:24:26.54 Vacuum too high -- waiting up to 40s @08:24:26.81 SS.seek 25.00ml @08:24:27.79 Vacuum too high -- waiting up to 40s @08:24:28.05 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:24:28.06 SS.reconfigure forSampling @08:24:28.09 SS.seek 25.00ml @08:24:29.14 Vacuum too high -- waiting up to 40s @08:24:29.40 Intake @47.4psia, vacuum from 20.0 to 24.0psi @08:24:29.41 SS.reconfigure forSampling @08:24:29.45 SS.seek 25.00ml @08:24:30.51 Vacuum too high -- waiting up to 40s @08:24:30.77 Intake @47.3psia, vacuum from 20.0 to 24.0psi @08:24:30.78 SS.reconfigure forSampling @08:24:30.81 SS.seek 25.00ml @08:24:31.98 Vacuum too high -- waiting up to 40s @08:24:32.46 Intake @48.9psia, vacuum from 20.0 to 24.0psi @08:24:32.47 SS.reconfigure forSampling @08:24:32.50 SS.seek 25.00ml @08:24:33.76 Vacuum too high -- waiting up to 40s @08:24:34.02 Intake @48.1psia, vacuum from 20.0 to 24.0psi @08:24:34.03 SS.reconfigure forSampling @08:24:34.06 SS.seek 25.00ml @08:24:35.10 Vacuum too high -- waiting up to 40s @08:24:35.36 Intake @48.0psia, vacuum from 20.0 to 24.0psi @08:24:35.37 SS.reconfigure forSampling @08:24:35.41 SS.seek 25.00ml @08:24:36.35 Vacuum too high -- waiting up to 40s @08:24:36.61 Intake @47.9psia, vacuum from 20.0 to 24.0psi @08:24:36.62 SS.reconfigure forSampling @08:24:36.65 SS.seek 25.00ml @08:24:37.57 Vacuum too high -- waiting up to 40s @08:24:37.83 Intake @47.7psia, vacuum from 20.0 to 24.0psi @08:24:37.84 SS.reconfigure forSampling @08:24:37.87 SS.seek 25.00ml @08:24:38.92 Vacuum too high -- waiting up to 40s @08:24:39.18 Intake @47.5psia, vacuum from 20.0 to 24.0psi @08:24:39.19 SS.reconfigure forSampling @08:24:39.22 SS.seek 25.00ml @08:24:40.29 Vacuum too high -- waiting up to 40s @08:24:40.55 Intake @47.4psia, vacuum from 20.0 to 24.0psi @08:24:40.56 SS.reconfigure forSampling @08:24:40.59 SS.seek 25.00ml @08:24:41.60 Vacuum too high -- waiting up to 40s @08:24:42.07 Intake @47.9psia, vacuum from 20.0 to 24.0psi @08:24:42.08 SS.reconfigure forSampling @08:24:42.11 SS.seek 25.00ml @08:24:43.29 Vacuum too high -- waiting up to 40s @08:24:43.55 Intake @48.1psia, vacuum from 20.0 to 24.0psi @08:24:43.56 SS.reconfigure forSampling @08:24:43.59 SS.seek 25.00ml @08:24:44.57 Vacuum too high -- waiting up to 40s @08:24:44.83 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:24:44.84 SS.reconfigure forSampling @08:24:44.87 SS.seek 25.00ml @08:24:45.95 Vacuum too high -- waiting up to 40s @08:24:47.00 Intake @48.8psia, vacuum from 20.0 to 24.0psi @08:24:47.01 SS.reconfigure forSampling @08:24:47.05 SS.seek 25.00ml @08:24:48.57 Vacuum too high -- waiting up to 40s @08:24:48.83 Intake @47.7psia, vacuum from 20.0 to 24.0psi @08:24:48.84 SS.reconfigure forSampling @08:24:48.87 SS.seek 25.00ml @08:24:49.85 Vacuum too high -- waiting up to 40s @08:24:50.11 Intake @47.4psia, vacuum from 20.0 to 24.0psi @08:24:50.12 SS.reconfigure forSampling @08:24:50.16 SS.seek 25.00ml @08:24:51.13 Vacuum too high -- waiting up to 40s @08:24:51.39 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:24:51.40 SS.reconfigure forSampling @08:24:51.44 SS.seek 25.00ml @08:24:52.38 Vacuum too high -- waiting up to 40s @08:24:52.64 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:24:52.65 SS.reconfigure forSampling @08:24:52.69 SS.seek 25.00ml @08:24:53.63 Vacuum too high -- waiting up to 40s @08:24:53.89 Intake @47.4psia, vacuum from 20.0 to 24.0psi @08:24:53.90 SS.reconfigure forSampling @08:24:53.94 SS.seek 25.00ml @08:24:54.85 Vacuum too high -- waiting up to 40s @08:24:55.11 Intake @47.2psia, vacuum from 20.0 to 24.0psi @08:24:55.12 SS.reconfigure forSampling @08:24:55.16 SS.seek 25.00ml @08:24:56.10 Vacuum too high -- waiting up to 40s @08:24:56.36 Intake @47.4psia, vacuum from 20.0 to 24.0psi @08:24:56.37 SS.reconfigure forSampling @08:24:56.40 SS.seek 25.00ml @08:24:57.42 Vacuum too high -- waiting up to 40s @08:24:57.89 Intake @48.7psia, vacuum from 20.0 to 24.0psi @08:24:57.90 SS.reconfigure forSampling @08:24:57.94 SS.seek 25.00ml @08:24:58.98 Vacuum too high -- waiting up to 40s @08:24:59.24 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:24:59.25 SS.reconfigure forSampling @08:24:59.28 SS.seek 25.00ml @08:25:00.17 Vacuum too high -- waiting up to 40s @08:25:00.43 Intake @47.7psia, vacuum from 20.0 to 24.0psi @08:25:00.44 SS.reconfigure forSampling @08:25:00.47 SS.seek 25.00ml @08:25:01.35 Vacuum too high -- waiting up to 40s @08:25:01.61 Intake @48.0psia, vacuum from 20.0 to 24.0psi @08:25:01.62 SS.reconfigure forSampling @08:25:01.66 SS.seek 25.00ml @08:25:02.60 Vacuum too high -- waiting up to 40s @08:25:02.86 Intake @47.7psia, vacuum from 20.0 to 24.0psi @08:25:02.87 SS.reconfigure forSampling @08:25:02.90 SS.seek 25.00ml @08:25:03.79 Vacuum too high -- waiting up to 40s @08:25:04.05 Intake @47.7psia, vacuum from 20.0 to 24.0psi @08:25:04.06 SS.reconfigure forSampling @08:25:04.09 SS.seek 25.00ml @08:25:05.01 Vacuum too high -- waiting up to 40s @08:25:05.27 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:25:05.28 SS.reconfigure forSampling @08:25:05.31 SS.seek 25.00ml @08:25:06.20 Vacuum too high -- waiting up to 40s @08:25:06.47 SS.seek 25.00ml @08:25:07.29 Vacuum too high -- waiting up to 40s @08:25:07.55 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:25:07.56 SS.reconfigure forSampling @08:25:07.59 SS.seek 25.00ml @08:25:08.57 Vacuum too high -- waiting up to 40s @08:25:09.04 Intake @48.0psia, vacuum from 20.0 to 24.0psi @08:25:09.05 SS.reconfigure forSampling @08:25:09.08 SS.seek 25.00ml @08:25:10.07 Vacuum too high -- waiting up to 40s @08:25:10.55 Intake @48.3psia, vacuum from 20.0 to 24.0psi @08:25:10.56 SS.reconfigure forSampling @08:25:10.60 SS.seek 25.00ml @08:25:11.66 Vacuum too high -- waiting up to 40s @08:25:11.92 Intake @47.8psia, vacuum from 20.0 to 24.0psi @08:25:11.93 SS.reconfigure forSampling @08:25:11.96 SS.seek 25.00ml @08:25:12.79 Vacuum too high -- waiting up to 40s @08:25:13.05 Intake @47.7psia, vacuum from 20.0 to 24.0psi @08:25:13.06 SS.reconfigure forSampling @08:25:13.09 SS.seek 25.00ml @08:25:14.04 Vacuum too high -- waiting up to 40s @08:25:14.30 Intake @47.5psia, vacuum from 20.0 to 24.0psi @08:25:14.31 SS.reconfigure forSampling @08:25:14.34 SS.seek 25.00ml @08:25:15.20 Vacuum too high -- waiting up to 40s @08:25:15.46 Intake @47.4psia, vacuum from 20.0 to 24.0psi @08:25:15.47 SS.reconfigure forSampling @08:25:15.50 SS.seek 25.00ml @08:25:16.35 Vacuum too high -- waiting up to 40s @08:25:16.61 Intake @47.3psia, vacuum from 20.0 to 24.0psi @08:25:16.62 SS.reconfigure forSampling @08:25:16.65 SS.seek 25.00ml @08:25:17.51 Vacuum too high -- waiting up to 40s @08:25:18.34 Intake @47.7psia, vacuum from 20.0 to 24.0psi @08:25:18.35 SS.reconfigure forSampling @08:25:18.39 SS.seek 25.00ml @08:25:19.98 Vacuum too high -- waiting up to 40s @08:25:20.45 Intake @48.5psia, vacuum from 20.0 to 24.0psi @08:25:20.46 SS.reconfigure forSampling @08:25:20.50 SS.seek 25.00ml @08:25:21.63 Vacuum too high -- waiting up to 40s @08:25:21.89 Intake @47.7psia, vacuum from 20.0 to 24.0psi @08:25:21.90 SS.reconfigure forSampling @08:25:21.93 SS.seek 25.00ml @08:25:22.82 Vacuum too high -- waiting up to 40s @08:25:23.08 Intake @47.3psia, vacuum from 20.0 to 24.0psi @08:25:23.09 SS.reconfigure forSampling @08:25:23.13 SS.seek 25.00ml @08:25:24.04 Vacuum too high -- waiting up to 40s @08:25:24.52 Intake @47.7psia, vacuum from 20.0 to 24.0psi @08:25:24.54 SS.reconfigure forSampling @08:25:24.57 SS.seek 25.00ml @08:25:25.70 Vacuum too high -- waiting up to 40s @08:25:26.18 SS.seek 25.00ml @08:25:27.29 Vacuum too high -- waiting up to 40s @08:25:27.55 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:25:27.56 SS.reconfigure forSampling @08:25:27.59 SS.seek 25.00ml @08:25:28.35 Vacuum too high -- waiting up to 40s @08:25:28.61 Intake @47.7psia, vacuum from 20.0 to 24.0psi @08:25:28.62 SS.reconfigure forSampling @08:25:28.65 SS.seek 25.00ml @08:25:29.48 Vacuum too high -- waiting up to 40s @08:25:29.95 Intake @47.9psia, vacuum from 20.0 to 24.0psi @08:25:29.96 SS.reconfigure forSampling @08:25:30.00 SS.seek 25.00ml @08:25:31.16 Vacuum too high -- waiting up to 40s @08:25:31.63 Intake @48.4psia, vacuum from 20.0 to 24.0psi @08:25:31.64 SS.reconfigure forSampling @08:25:31.67 SS.seek 25.00ml @08:25:32.73 Vacuum too high -- waiting up to 40s @08:25:32.99 Intake @48.0psia, vacuum from 20.0 to 24.0psi @08:25:33.00 SS.reconfigure forSampling @08:25:33.03 SS.seek 25.00ml @08:25:33.88 Vacuum too high -- waiting up to 40s @08:25:34.14 Intake @47.7psia, vacuum from 20.0 to 24.0psi @08:25:34.15 SS.reconfigure forSampling @08:25:34.18 SS.seek 25.00ml @08:25:35.18 Vacuum too high -- waiting up to 40s @08:25:35.45 SS.seek 25.00ml @08:25:36.35 Vacuum too high -- waiting up to 40s @08:25:36.83 Intake @47.5psia, vacuum from 20.0 to 24.0psi @08:25:36.84 SS.reconfigure forSampling @08:25:36.87 SS.seek 25.00ml @08:25:37.98 Vacuum too high -- waiting up to 40s @08:25:38.24 Intake @47.4psia, vacuum from 20.0 to 24.0psi @08:25:38.25 SS.reconfigure forSampling @08:25:38.28 SS.seek 25.00ml @08:25:39.16 Vacuum too high -- waiting up to 40s @08:25:39.43 Intake @47.5psia, vacuum from 20.0 to 24.0psi @08:25:39.44 SS.reconfigure forSampling @08:25:39.47 SS.seek 25.00ml @08:25:40.26 Vacuum too high -- waiting up to 40s @08:25:40.73 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:25:40.74 SS.reconfigure forSampling @08:25:40.77 SS.seek 25.00ml @08:25:41.79 Vacuum too high -- waiting up to 40s @08:25:42.27 Intake @48.7psia, vacuum from 20.0 to 24.0psi @08:25:42.28 SS.reconfigure forSampling @08:25:42.31 SS.seek 25.00ml @08:25:43.29 Vacuum too high -- waiting up to 40s @08:25:43.55 Intake @48.4psia, vacuum from 20.0 to 24.0psi @08:25:43.56 SS.reconfigure forSampling @08:25:43.59 SS.seek 25.00ml @08:25:44.35 Vacuum too high -- waiting up to 40s @08:25:44.61 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:25:44.62 SS.reconfigure forSampling @08:25:44.66 SS.seek 25.00ml @08:25:45.57 Vacuum too high -- waiting up to 40s @08:25:45.83 Intake @47.7psia, vacuum from 20.0 to 24.0psi @08:25:45.84 SS.reconfigure forSampling @08:25:45.88 SS.seek 25.00ml @08:25:46.60 Vacuum too high -- waiting up to 40s @08:25:47.08 Intake @47.7psia, vacuum from 20.0 to 24.0psi @08:25:47.09 SS.reconfigure forSampling @08:25:47.12 SS.seek 25.00ml @08:25:48.16 Vacuum too high -- waiting up to 40s @08:25:48.97 Intake @47.5psia, vacuum from 20.0 to 24.0psi @08:25:48.98 SS.reconfigure forSampling @08:25:49.02 SS.seek 25.00ml @08:25:50.32 Vacuum too high -- waiting up to 40s @08:25:50.58 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:25:50.59 SS.reconfigure forSampling @08:25:50.62 SS.seek 25.00ml @08:25:51.48 Vacuum too high -- waiting up to 40s @08:25:51.96 Intake @47.7psia, vacuum from 20.0 to 24.0psi @08:25:51.97 SS.reconfigure forSampling @08:25:52.01 SS.seek 25.00ml @08:25:53.07 Vacuum too high -- waiting up to 40s @08:25:53.54 Intake @48.7psia, vacuum from 20.0 to 24.0psi @08:25:53.55 SS.reconfigure forSampling @08:25:53.58 SS.seek 25.00ml @08:25:54.60 Vacuum too high -- waiting up to 40s @08:25:55.09 Intake @48.4psia, vacuum from 20.0 to 24.0psi @08:25:55.10 SS.reconfigure forSampling @08:25:55.14 SS.seek 25.00ml @08:25:56.07 Vacuum too high -- waiting up to 40s @08:25:56.33 Intake @47.7psia, vacuum from 20.0 to 24.0psi @08:25:56.34 SS.reconfigure forSampling @08:25:56.38 SS.seek 25.00ml @08:25:57.16 Vacuum too high -- waiting up to 40s @08:25:57.63 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:25:57.64 SS.reconfigure forSampling @08:25:57.67 SS.seek 25.00ml @08:25:58.72 Vacuum too high -- waiting up to 40s @08:25:59.19 Intake @47.7psia, vacuum from 20.0 to 24.0psi @08:25:59.20 SS.reconfigure forSampling @08:25:59.23 SS.seek 25.00ml @08:26:00.26 Vacuum too high -- waiting up to 40s @08:26:00.73 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:26:00.74 SS.reconfigure forSampling @08:26:00.78 SS.seek 25.00ml @08:26:01.76 Vacuum too high -- waiting up to 40s @08:26:02.24 Intake @47.8psia, vacuum from 20.0 to 24.0psi @08:26:02.25 SS.reconfigure forSampling @08:26:02.28 SS.seek 25.00ml @08:26:03.22 Vacuum too high -- waiting up to 40s @08:26:03.48 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:26:03.49 SS.reconfigure forSampling @08:26:03.52 SS.seek 25.00ml @08:26:04.29 Vacuum too high -- waiting up to 40s @08:26:04.55 Intake @47.3psia, vacuum from 20.0 to 24.0psi @08:26:04.56 SS.reconfigure forSampling @08:26:04.60 SS.seek 25.00ml @08:26:05.41 Vacuum too high -- waiting up to 40s @08:26:05.89 SS.seek 25.00ml @08:26:06.85 Vacuum too high -- waiting up to 40s @08:26:07.33 Intake @48.4psia, vacuum from 20.0 to 24.0psi @08:26:07.34 SS.reconfigure forSampling @08:26:07.37 SS.seek 25.00ml @08:26:08.22 Vacuum too high -- waiting up to 40s @08:26:08.48 Intake @48.5psia, vacuum from 20.0 to 24.0psi @08:26:08.49 SS.reconfigure forSampling @08:26:08.52 SS.seek 25.00ml @08:26:09.19 Vacuum too high -- waiting up to 40s @08:26:09.45 Intake @47.7psia, vacuum from 20.0 to 24.0psi @08:26:09.46 SS.reconfigure forSampling @08:26:09.50 SS.seek 25.00ml @08:26:10.32 Vacuum too high -- waiting up to 40s @08:26:10.80 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:26:10.81 SS.reconfigure forSampling @08:26:10.84 SS.seek 25.00ml @08:26:11.97 Vacuum too high -- waiting up to 40s @08:26:12.45 Intake @47.9psia, vacuum from 20.0 to 24.0psi @08:26:12.46 SS.reconfigure forSampling @08:26:12.50 SS.seek 25.00ml @08:26:13.38 Vacuum too high -- waiting up to 40s @08:26:13.64 Intake @47.7psia, vacuum from 20.0 to 24.0psi @08:26:13.65 SS.reconfigure forSampling @08:26:13.68 SS.seek 25.00ml @08:26:14.32 Vacuum too high -- waiting up to 40s @08:26:14.58 Intake @47.7psia, vacuum from 20.0 to 24.0psi @08:26:14.59 SS.reconfigure forSampling @08:26:14.62 SS.seek 25.00ml @08:26:15.29 Vacuum too high -- waiting up to 40s @08:26:15.55 Intake @47.5psia, vacuum from 20.0 to 24.0psi @08:26:15.56 SS.reconfigure forSampling @08:26:15.59 SS.seek 25.00ml @08:26:16.38 Vacuum too high -- waiting up to 40s @08:26:16.85 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:26:16.86 SS.reconfigure forSampling @08:26:16.89 SS.seek 25.00ml @08:26:18.91 Vacuum too high -- waiting up to 40s @08:26:19.39 SS.seek 25.00ml @08:26:20.32 Vacuum too high -- waiting up to 40s @08:26:20.79 Intake @48.2psia, vacuum from 20.0 to 24.0psi @08:26:20.80 SS.reconfigure forSampling @08:26:20.83 SS.seek 25.00ml @08:26:21.69 Vacuum too high -- waiting up to 40s @08:26:22.16 Intake @48.1psia, vacuum from 20.0 to 24.0psi @08:26:22.17 SS.reconfigure forSampling @08:26:22.20 SS.seek 25.00ml @08:26:23.07 Vacuum too high -- waiting up to 40s @08:26:23.33 Intake @47.7psia, vacuum from 20.0 to 24.0psi @08:26:23.34 SS.reconfigure forSampling @08:26:23.37 SS.seek 25.00ml @08:26:24.19 Vacuum too high -- waiting up to 40s @08:26:24.46 Intake @47.8psia, vacuum from 20.0 to 24.0psi @08:26:24.47 SS.reconfigure forSampling @08:26:24.50 SS.seek 25.00ml @08:26:25.22 Vacuum too high -- waiting up to 40s @08:26:25.69 Intake @48.0psia, vacuum from 20.0 to 24.0psi @08:26:25.70 SS.reconfigure forSampling @08:26:25.73 SS.seek 25.00ml @08:26:26.63 Vacuum too high -- waiting up to 40s @08:26:26.89 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:26:26.90 SS.reconfigure forSampling @08:26:26.93 SS.seek 25.00ml @08:26:27.66 Vacuum too high -- waiting up to 40s @08:26:28.13 Intake @47.3psia, vacuum from 20.0 to 24.0psi @08:26:28.14 SS.reconfigure forSampling @08:26:28.17 SS.seek 25.00ml @08:26:29.16 Vacuum too high -- waiting up to 40s @08:26:29.64 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:26:29.65 SS.reconfigure forSampling @08:26:29.69 SS.seek 25.00ml @08:26:30.75 Vacuum too high -- waiting up to 40s @08:26:31.23 Intake @47.9psia, vacuum from 20.0 to 24.0psi @08:26:31.24 SS.reconfigure forSampling @08:26:31.27 SS.seek 25.00ml @08:26:32.19 Vacuum too high -- waiting up to 40s @08:26:32.66 Intake @47.8psia, vacuum from 20.0 to 24.0psi @08:26:32.67 SS.reconfigure forSampling @08:26:32.71 SS.seek 25.00ml @08:26:33.69 Vacuum too high -- waiting up to 40s @08:26:34.17 Intake @48.3psia, vacuum from 20.0 to 24.0psi @08:26:34.18 SS.reconfigure forSampling @08:26:34.22 SS.seek 25.00ml @08:26:35.13 Vacuum too high -- waiting up to 40s @08:26:35.60 Intake @48.4psia, vacuum from 20.0 to 24.0psi @08:26:35.61 SS.reconfigure forSampling @08:26:35.64 SS.seek 25.00ml @08:26:36.44 Vacuum too high -- waiting up to 40s @08:26:36.70 Intake @48.0psia, vacuum from 20.0 to 24.0psi @08:26:36.71 SS.reconfigure forSampling @08:26:36.74 SS.seek 25.00ml @08:26:37.50 Vacuum too high -- waiting up to 40s @08:26:37.98 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:26:37.99 SS.reconfigure forSampling @08:26:38.02 SS.seek 25.00ml @08:26:39.07 Vacuum too high -- waiting up to 40s @08:26:39.55 Intake @47.8psia, vacuum from 20.0 to 24.0psi @08:26:39.56 SS.reconfigure forSampling @08:26:39.59 SS.seek 25.00ml @08:26:40.50 Vacuum too high -- waiting up to 40s @08:26:40.98 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:26:40.99 SS.reconfigure forSampling @08:26:41.02 SS.seek 25.00ml @08:26:42.00 Vacuum too high -- waiting up to 40s @08:26:42.47 Intake @47.5psia, vacuum from 20.0 to 24.0psi @08:26:42.48 SS.reconfigure forSampling @08:26:42.51 SS.seek 25.00ml @08:26:43.54 Vacuum too high -- waiting up to 40s @08:26:44.02 Intake @47.7psia, vacuum from 20.0 to 24.0psi @08:26:44.03 SS.reconfigure forSampling @08:26:44.06 SS.seek 25.00ml @08:26:45.10 Vacuum too high -- waiting up to 40s @08:26:45.58 Intake @47.7psia, vacuum from 20.0 to 24.0psi @08:26:45.59 SS.reconfigure forSampling @08:26:45.62 SS.seek 25.00ml @08:26:46.60 Vacuum too high -- waiting up to 40s @08:26:47.64 Intake @49.1psia, vacuum from 20.0 to 24.0psi @08:26:47.65 SS.reconfigure forSampling @08:26:47.69 SS.seek 25.00ml @08:26:48.36 Stroke completed -- waiting for pressure to equalize ... @08:26:50.18 Intake.select :close @08:26:50.33 SSV.dial :exhaust @08:26:51.14 Exhaust.select :open @08:26:51.25 SS.reconfigure SSexhale @08:26:51.29 SS.seek 0.00ml @08:27:00.59 Exhaust.select :close @08:27:00.68 SSV.dial CBV @08:27:00.70
CBV.dial SSV @08:27:01.51 Allowing 185s for 25.0ml of remaining 825.0ml @08:27:01.52 Intake.select :open @08:27:03.65 Intake @47.3psia, vacuum from 20.0 to 24.0psi @08:27:03.66 SS.reconfigure forSampling @08:27:03.70 SS.seek 25.00ml @08:27:05.41 Vacuum too high -- waiting up to 40s @08:27:05.88 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:27:05.89 SS.reconfigure forSampling @08:27:05.92 SS.seek 25.00ml @08:27:06.88 Vacuum too high -- waiting up to 40s @08:27:07.14 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:27:07.15 SS.reconfigure forSampling @08:27:07.18 SS.seek 25.00ml @08:27:07.94 Vacuum too high -- waiting up to 40s @08:27:08.20 Intake @47.3psia, vacuum from 20.0 to 24.0psi @08:27:08.21 SS.reconfigure forSampling @08:27:08.24 SS.seek 25.00ml @08:27:08.97 Vacuum too high -- waiting up to 40s @08:27:09.23 Intake @47.5psia, vacuum from 20.0 to 24.0psi @08:27:09.24 SS.reconfigure forSampling @08:27:09.27 SS.seek 25.00ml @08:27:10.03 Vacuum too high -- waiting up to 40s @08:27:10.51 Intake @48.3psia, vacuum from 20.0 to 24.0psi @08:27:10.52 SS.reconfigure forSampling @08:27:10.55 SS.seek 25.00ml @08:27:11.50 Vacuum too high -- waiting up to 40s @08:27:11.76 SS.seek 25.00ml @08:27:12.38 Vacuum too high -- waiting up to 40s @08:27:12.64 Intake @48.0psia, vacuum from 20.0 to 24.0psi @08:27:12.65 SS.reconfigure forSampling @08:27:12.69 SS.seek 25.00ml @08:27:13.47 Vacuum too high -- waiting up to 40s @08:27:13.73 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:27:13.74 SS.reconfigure forSampling @08:27:13.77 SS.seek 25.00ml @08:27:14.50 Vacuum too high -- waiting up to 40s @08:27:14.98 Intake @47.7psia, vacuum from 20.0 to 24.0psi @08:27:14.99 SS.reconfigure forSampling @08:27:15.02 SS.seek 25.00ml @08:27:16.06 Vacuum too high -- waiting up to 40s @08:27:16.53 Intake @47.8psia, vacuum from 20.0 to 24.0psi @08:27:16.54 SS.reconfigure forSampling @08:27:16.57 SS.seek 25.00ml @08:27:17.50 Vacuum too high -- waiting up to 40s @08:27:17.98 Intake @47.9psia, vacuum from 20.0 to 24.0psi @08:27:17.99 SS.reconfigure forSampling @08:27:18.02 SS.seek 25.00ml @08:27:18.97 Vacuum too high -- waiting up to 40s @08:27:19.23 Intake @47.7psia, vacuum from 20.0 to 24.0psi @08:27:19.24 SS.reconfigure forSampling @08:27:19.27 SS.seek 25.00ml @08:27:20.00 Vacuum too high -- waiting up to 40s @08:27:20.48 Intake @47.8psia, vacuum from 20.0 to 24.0psi @08:27:20.49 SS.reconfigure forSampling @08:27:20.52 SS.seek 25.00ml @08:27:21.31 Vacuum too high -- waiting up to 40s @08:27:21.57 Intake @47.7psia, vacuum from 20.0 to 24.0psi @08:27:21.58 SS.reconfigure forSampling @08:27:21.61 SS.seek 25.00ml @08:27:22.38 Vacuum too high -- waiting up to 40s @08:27:22.87 SS.seek 25.00ml @08:27:23.81 Vacuum too high -- waiting up to 40s @08:27:24.29 Intake @48.4psia, vacuum from 20.0 to 24.0psi @08:27:24.30 SS.reconfigure forSampling @08:27:24.33 SS.seek 25.00ml @08:27:25.25 Vacuum too high -- waiting up to 40s @08:27:25.51 Intake @48.2psia, vacuum from 20.0 to 24.0psi @08:27:25.52 SS.reconfigure forSampling @08:27:25.55 SS.seek 25.00ml @08:27:26.16 Vacuum too high -- waiting up to 40s @08:27:26.42 Intake @47.8psia, vacuum from 20.0 to 24.0psi @08:27:26.43 SS.reconfigure forSampling @08:27:26.46 SS.seek 25.00ml @08:27:27.13 Vacuum too high -- waiting up to 40s @08:27:27.94 Intake @47.4psia, vacuum from 20.0 to 24.0psi @08:27:27.95 SS.reconfigure forSampling @08:27:27.99 SS.seek 25.00ml @08:27:29.10 Vacuum too high -- waiting up to 40s @08:27:29.58 Intake @47.3psia, vacuum from 20.0 to 24.0psi @08:27:29.59 SS.reconfigure forSampling @08:27:29.63 SS.seek 25.00ml @08:27:30.60 Vacuum too high -- waiting up to 40s @08:27:31.07 Intake @48.2psia, vacuum from 20.0 to 24.0psi @08:27:31.08 SS.reconfigure forSampling @08:27:31.12 SS.seek 25.00ml @08:27:32.10 Vacuum too high -- waiting up to 40s @08:27:32.59 Intake @48.6psia, vacuum from 20.0 to 24.0psi @08:27:32.60 SS.reconfigure forSampling @08:27:32.63 SS.seek 25.00ml @08:27:33.50 Vacuum too high -- waiting up to 40s @08:27:33.76 Intake @47.8psia, vacuum from 20.0 to 24.0psi @08:27:33.77 SS.reconfigure forSampling @08:27:33.80 SS.seek 25.00ml @08:27:34.60 Vacuum too high -- waiting up to 40s @08:27:35.08 Intake @47.5psia, vacuum from 20.0 to 24.0psi @08:27:35.09 SS.reconfigure forSampling @08:27:35.13 SS.seek 25.00ml @08:27:36.06 Vacuum too high -- waiting up to 40s @08:27:36.53 Intake @47.4psia, vacuum from 20.0 to 24.0psi @08:27:36.54 SS.reconfigure forSampling @08:27:36.57 SS.seek 25.00ml @08:27:37.50 Vacuum too high -- waiting up to 40s @08:27:37.97 Intake @47.4psia, vacuum from 20.0 to 24.0psi @08:27:37.98 SS.reconfigure forSampling @08:27:38.01 SS.seek 25.00ml @08:27:38.91 Vacuum too high -- waiting up to 40s @08:27:39.38 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:27:39.39 SS.reconfigure forSampling @08:27:39.42 SS.seek 25.00ml @08:27:40.28 Vacuum too high -- waiting up to 40s @08:27:40.75 Intake @47.7psia, vacuum from 20.0 to 24.0psi @08:27:40.76 SS.reconfigure forSampling @08:27:40.79 SS.seek 25.00ml @08:27:41.72 Vacuum too high -- waiting up to 40s @08:27:42.19 Intake @47.7psia, vacuum from 20.0 to 24.0psi @08:27:42.20 SS.reconfigure forSampling @08:27:42.23 SS.seek 25.00ml @08:27:43.19 Vacuum too high -- waiting up to 40s @08:27:43.66 Intake @48.1psia, vacuum from 20.0 to 24.0psi @08:27:43.67 SS.reconfigure forSampling @08:27:43.70 SS.seek 25.00ml @08:27:44.50 Vacuum too high -- waiting up to 40s @08:27:44.76 Intake @48.2psia, vacuum from 20.0 to 24.0psi @08:27:44.77 SS.reconfigure forSampling @08:27:44.80 SS.seek 25.00ml @08:27:45.50 Vacuum too high -- waiting up to 40s @08:27:45.98 Intake @47.8psia, vacuum from 20.0 to 24.0psi @08:27:45.99 SS.reconfigure forSampling @08:27:46.02 SS.seek 25.00ml @08:27:46.91 Vacuum too high -- waiting up to 40s @08:27:47.17 Intake @47.4psia, vacuum from 20.0 to 24.0psi @08:27:47.18 SS.reconfigure forSampling @08:27:47.21 SS.seek 25.00ml @08:27:47.88 Vacuum too high -- waiting up to 40s @08:27:48.35 Intake @47.3psia, vacuum from 20.0 to 24.0psi @08:27:48.36 SS.reconfigure forSampling @08:27:48.39 SS.seek 25.00ml @08:27:49.28 Vacuum too high -- waiting up to 40s @08:27:49.75 Intake @47.3psia, vacuum from 20.0 to 24.0psi @08:27:49.76 SS.reconfigure forSampling @08:27:49.79 SS.seek 25.00ml @08:27:50.66 Vacuum too high -- waiting up to 40s @08:27:51.14 Intake @47.5psia, vacuum from 20.0 to 24.0psi @08:27:51.15 SS.reconfigure forSampling @08:27:51.18 SS.seek 25.00ml @08:27:52.13 Vacuum too high -- waiting up to 40s @08:27:52.60 Intake @47.4psia, vacuum from 20.0 to 24.0psi @08:27:52.61 SS.reconfigure forSampling @08:27:52.64 SS.seek 25.00ml @08:27:53.50 Vacuum too high -- waiting up to 40s @08:27:53.98 Intake @47.7psia, vacuum from 20.0 to 24.0psi @08:27:53.99 SS.reconfigure forSampling @08:27:54.02 SS.seek 25.00ml @08:27:55.00 Vacuum too high -- waiting up to 40s @08:27:56.07 Intake @48.0psia, vacuum from 20.0 to 24.0psi @08:27:56.08 SS.reconfigure forSampling @08:27:56.12 SS.seek 25.00ml @08:27:57.31 Vacuum too high -- waiting up to 40s @08:27:57.78 Intake @47.5psia, vacuum from 20.0 to 24.0psi @08:27:57.79 SS.reconfigure forSampling @08:27:57.82 SS.seek 25.00ml @08:27:58.72 Vacuum too high -- waiting up to 40s @08:27:59.21 Intake @47.8psia, vacuum from 20.0 to 24.0psi @08:27:59.22 SS.reconfigure forSampling @08:27:59.25 SS.seek 25.00ml @08:28:00.16 Vacuum too high -- waiting up to 40s @08:28:00.64 Intake @47.9psia, vacuum from 20.0 to 24.0psi @08:28:00.65 SS.reconfigure forSampling @08:28:00.69 SS.seek 25.00ml @08:28:01.56 Vacuum too high -- waiting up to 40s @08:28:02.04 Intake @47.5psia, vacuum from 20.0 to 24.0psi @08:28:02.05 SS.reconfigure forSampling @08:28:02.08 SS.seek 25.00ml @08:28:02.97 Vacuum too high -- waiting up to 40s @08:28:03.44 Intake @47.4psia, vacuum from 20.0 to 24.0psi @08:28:03.45 SS.reconfigure forSampling @08:28:03.49 SS.seek 25.00ml @08:28:04.41 Vacuum too high -- waiting up to 40s @08:28:04.69 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:28:04.70 SS.reconfigure forSampling @08:28:04.73 SS.seek 25.00ml @08:28:05.41 Vacuum too high -- waiting up to 40s @08:28:05.88 Intake @48.1psia, vacuum from 20.0 to 24.0psi @08:28:05.89 SS.reconfigure forSampling @08:28:05.92 SS.seek 25.00ml @08:28:06.78 Vacuum too high -- waiting up to 40s @08:28:07.26 SS.seek 25.00ml @08:28:08.06 Vacuum too high -- waiting up to 40s @08:28:08.32 Intake @47.8psia, vacuum from 20.0 to 24.0psi @08:28:08.33 SS.reconfigure forSampling @08:28:08.36 SS.seek 25.00ml @08:28:09.03 Vacuum too high -- waiting up to 40s @08:28:09.50 Intake @47.7psia, vacuum from 20.0 to 24.0psi @08:28:09.51 SS.reconfigure forSampling @08:28:09.55 SS.seek 25.00ml @08:28:10.44 Vacuum too high -- waiting up to 40s @08:28:10.91 Intake @47.7psia, vacuum from 20.0 to 24.0psi @08:28:10.92 SS.reconfigure forSampling @08:28:10.96 SS.seek 25.00ml @08:28:11.75 Vacuum too high -- waiting up to 40s @08:28:12.23 Intake @47.4psia, vacuum from 20.0 to 24.0psi @08:28:12.24 SS.reconfigure forSampling @08:28:12.27 SS.seek 25.00ml @08:28:13.12 Vacuum too high -- waiting up to 40s @08:28:13.38 Intake @47.1psia, vacuum from 20.0 to 24.0psi @08:28:13.39 SS.reconfigure forSampling @08:28:13.42 SS.seek 25.00ml @08:28:14.09 Vacuum too high -- waiting up to 40s @08:28:14.57 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:28:14.58 SS.reconfigure forSampling @08:28:14.61 SS.seek 25.00ml @08:28:15.56 Vacuum too high -- waiting up to 40s @08:28:16.02 Intake @48.4psia, vacuum from 20.0 to 24.0psi @08:28:16.04 SS.reconfigure forSampling @08:28:16.07 SS.seek 25.00ml @08:28:16.84 Vacuum too high -- waiting up to 40s @08:28:17.31 Intake @47.7psia, vacuum from 20.0 to 24.0psi @08:28:17.32 SS.reconfigure forSampling @08:28:17.36 SS.seek 25.00ml @08:28:18.25 Vacuum too high -- waiting up to 40s @08:28:18.51 Intake @47.1psia, vacuum from 20.0 to 24.0psi @08:28:18.52 SS.reconfigure forSampling @08:28:18.55 SS.seek 25.00ml @08:28:19.22 Vacuum too high -- waiting up to 40s @08:28:19.70 Intake @47.5psia, vacuum from 20.0 to 24.0psi @08:28:19.71 SS.reconfigure forSampling @08:28:19.75 SS.seek 25.00ml @08:28:20.53 Vacuum too high -- waiting up to 40s @08:28:21.00 Intake @47.8psia, vacuum from 20.0 to 24.0psi @08:28:21.01 SS.reconfigure forSampling @08:28:21.04 SS.seek 25.00ml @08:28:21.87 Vacuum too high -- waiting up to 40s @08:28:22.35 Intake @47.7psia, vacuum from 20.0 to 24.0psi @08:28:22.36 SS.reconfigure forSampling @08:28:22.99 SS.seek 25.00ml @08:28:24.25 Vacuum too high -- waiting up to 40s @08:28:24.73 Intake @47.8psia, vacuum from 20.0 to 24.0psi @08:28:24.74 SS.reconfigure forSampling @08:28:24.77 SS.seek 25.00ml @08:28:25.65 Vacuum too high -- waiting up to 40s @08:28:26.13 Intake @47.7psia, vacuum from 20.0 to 24.0psi @08:28:26.14 SS.reconfigure forSampling @08:28:26.17 SS.seek 25.00ml @08:28:27.03 Vacuum too high -- waiting up to 40s @08:28:27.51 Intake @47.5psia, vacuum from 20.0 to 24.0psi @08:28:27.52 SS.reconfigure forSampling @08:28:27.55 SS.seek 25.00ml @08:28:28.37 Vacuum too high -- waiting up to 40s @08:28:28.85 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:28:28.86 SS.reconfigure forSampling @08:28:28.89 SS.seek 25.00ml @08:28:29.72 Vacuum too high -- waiting up to 40s @08:28:30.19 Intake @47.4psia, vacuum from 20.0 to 24.0psi @08:28:30.20 SS.reconfigure forSampling @08:28:30.24 SS.seek 25.00ml @08:28:31.09 Vacuum too high -- waiting up to 40s @08:28:31.57 Intake @47.9psia, vacuum from 20.0 to 24.0psi @08:28:31.58 SS.reconfigure forSampling @08:28:31.62 SS.seek 25.00ml @08:28:32.53 Vacuum too high -- waiting up to 40s @08:28:33.00 Intake @48.2psia, vacuum from 20.0 to 24.0psi @08:28:33.01 SS.reconfigure forSampling @08:28:33.04 SS.seek 25.00ml @08:28:33.87 Vacuum too high -- waiting up to 40s @08:28:34.13 Intake @47.9psia, vacuum from 20.0 to 24.0psi @08:28:34.14 SS.reconfigure forSampling @08:28:34.17 SS.seek 25.00ml @08:28:34.78 Vacuum too high -- waiting up to 40s @08:28:35.25 Intake @47.7psia, vacuum from 20.0 to 24.0psi @08:28:35.26 SS.reconfigure forSampling @08:28:35.29 SS.seek 25.00ml @08:28:36.00 Vacuum too high -- waiting up to 40s @08:28:36.48 Intake @47.7psia, vacuum from 20.0 to 24.0psi @08:28:36.49 SS.reconfigure forSampling @08:28:36.52 SS.seek 25.00ml @08:28:37.34 Vacuum too high -- waiting up to 40s @08:28:37.82 Intake @47.7psia, vacuum from 20.0 to 24.0psi @08:28:37.83 SS.reconfigure forSampling @08:28:37.86 SS.seek 25.00ml @08:28:38.69 Vacuum too high -- waiting up to 40s @08:28:39.16 Intake @48.1psia, vacuum from 20.0 to 24.0psi @08:28:39.17 SS.reconfigure forSampling @08:28:39.21 SS.seek 25.00ml @08:28:40.12 Vacuum too high -- waiting up to 40s @08:28:40.61 SS.seek 25.00ml @08:28:41.34 Vacuum too high -- waiting up to 40s @08:28:41.81 Intake @47.5psia, vacuum from 20.0 to 24.0psi @08:28:41.82 SS.reconfigure forSampling @08:28:41.86 SS.seek 25.00ml @08:28:42.72 Vacuum too high -- waiting up to 40s @08:28:42.98 Intake @47.2psia, vacuum from 20.0 to 24.0psi @08:28:42.99 SS.reconfigure forSampling @08:28:43.02 SS.seek 25.00ml @08:28:43.65 Vacuum too high -- waiting up to 40s @08:28:44.12 Intake @47.3psia, vacuum from 20.0 to 24.0psi @08:28:44.13 SS.reconfigure forSampling @08:28:44.16 SS.seek 25.00ml @08:28:44.87 Vacuum too high -- waiting up to 40s @08:28:45.35 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:28:45.36 SS.reconfigure forSampling @08:28:45.39 SS.seek 25.00ml @08:28:46.03 Vacuum too high -- waiting up to 40s @08:28:46.51 Intake @47.4psia, vacuum from 20.0 to 24.0psi @08:28:46.52 SS.reconfigure forSampling @08:28:46.55 SS.seek 25.00ml @08:28:47.28 Vacuum too high -- waiting up to 40s @08:28:47.76 Intake @47.7psia, vacuum from 20.0 to 24.0psi @08:28:47.77 SS.reconfigure forSampling @08:28:47.80 SS.seek 25.00ml @08:28:48.56 Vacuum too high -- waiting up to 40s @08:28:49.04 Intake @48.4psia, vacuum from 20.0 to 24.0psi @08:28:49.05 SS.reconfigure forSampling @08:28:49.70 SS.seek 25.00ml @08:28:50.83 Vacuum too high -- waiting up to 40s @08:28:51.10 Intake @47.7psia, vacuum from 20.0 to 24.0psi @08:28:51.11 SS.reconfigure forSampling @08:28:51.14 SS.seek 25.00ml @08:28:51.75 Vacuum too high -- waiting up to 40s @08:28:52.23 Intake @47.5psia, vacuum from 20.0 to 24.0psi @08:28:52.24 SS.reconfigure forSampling @08:28:52.27 SS.seek 25.00ml @08:28:53.15 Vacuum too high -- waiting up to 40s @08:28:53.63 Intake @47.8psia, vacuum from 20.0 to 24.0psi @08:28:53.64 SS.reconfigure forSampling @08:28:53.68 SS.seek 25.00ml @08:28:54.47 Vacuum too high -- waiting up to 40s @08:28:54.95 Intake @47.4psia, vacuum from 20.0 to 24.0psi @08:28:55.18 SS.reconfigure forSampling @08:28:55.22 SS.seek 25.00ml @08:28:56.40 Vacuum too high -- waiting up to 40s @08:28:56.87 Intake @46.8psia, vacuum from 20.0 to 24.0psi @08:28:56.88 SS.reconfigure forSampling @08:28:56.92 SS.seek 25.00ml @08:28:57.68 Vacuum too high -- waiting up to 40s @08:28:58.15 Intake @47.1psia, vacuum from 20.0 to 24.0psi @08:28:58.16 SS.reconfigure forSampling @08:28:58.19 SS.seek 25.00ml @08:28:59.00 Vacuum too high -- waiting up to 40s @08:28:59.48 SS.seek 25.00ml @08:29:00.31 Vacuum too high -- waiting up to 40s @08:29:00.79 SS.seek 25.00ml @08:29:01.50 Vacuum too high -- waiting up to 40s @08:29:01.97 Intake @47.9psia, vacuum from 20.0 to 24.0psi @08:29:01.98 SS.reconfigure forSampling @08:29:02.01 SS.seek 25.00ml @08:29:02.84 Vacuum too high -- waiting up to 40s @08:29:03.31 Intake @48.4psia, vacuum from 20.0 to 24.0psi @08:29:03.32 SS.reconfigure forSampling @08:29:03.35 SS.seek 25.00ml @08:29:04.06 Vacuum too high -- waiting up to 40s @08:29:04.32 Intake @47.4psia, vacuum from 20.0 to 24.0psi @08:29:04.33 SS.reconfigure forSampling @08:29:04.37 SS.seek 25.00ml @08:29:05.06 Vacuum too high -- waiting up to 40s @08:29:05.54 Intake @47.0psia, vacuum from 20.0 to 24.0psi @08:29:05.55 SS.reconfigure forSampling @08:29:05.58 SS.seek 25.00ml @08:29:06.34 Vacuum too high -- waiting up to 40s @08:29:06.82 Intake @47.1psia, vacuum from 20.0 to 24.0psi @08:29:06.83 SS.reconfigure forSampling @08:29:06.86 SS.seek 25.00ml @08:29:07.59 Vacuum too high -- waiting up to 40s @08:29:08.07 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:29:08.08 SS.reconfigure forSampling @08:29:08.11 SS.seek 25.00ml @08:29:08.84 Vacuum too high -- waiting up to 40s @08:29:09.31 SS.seek 25.00ml @08:29:10.06 Vacuum too high -- waiting up to 40s @08:29:10.54 Intake @48.2psia, vacuum from 20.0 to 24.0psi @08:29:10.55 SS.reconfigure forSampling @08:29:10.58 SS.seek 25.00ml @08:29:11.28 Vacuum too high -- waiting up to 40s @08:29:11.75 Intake @47.9psia, vacuum from 20.0 to 24.0psi @08:29:11.76 SS.reconfigure forSampling @08:29:11.79 SS.seek 25.00ml @08:29:12.47 Vacuum too high -- waiting up to 40s @08:29:12.72 Intake @47.5psia, vacuum from 20.0 to 24.0psi @08:29:12.73 SS.reconfigure forSampling @08:29:12.77 SS.seek 25.00ml @08:29:13.34 Vacuum too high -- waiting up to 40s @08:29:13.81 Intake @47.3psia, vacuum from 20.0 to 24.0psi @08:29:13.82 SS.reconfigure forSampling @08:29:13.85 SS.seek 25.00ml @08:29:14.60 Vacuum too high -- waiting up to 40s @08:29:15.07 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:29:15.08 SS.reconfigure forSampling @08:29:15.11 SS.seek 25.00ml @08:29:15.90 Vacuum too high -- waiting up to 40s @08:29:16.98 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:29:16.99 SS.reconfigure forSampling @08:29:17.02 SS.seek 25.00ml @08:29:18.14 Vacuum too high -- waiting up to 40s @08:29:18.62 Intake @47.5psia, vacuum from 20.0 to 24.0psi @08:29:18.63 SS.reconfigure forSampling @08:29:18.66 SS.seek 25.00ml @08:29:19.46 Vacuum too high -- waiting up to 40s @08:29:19.94 Intake @47.4psia, vacuum from 20.0 to 24.0psi @08:29:19.95 SS.reconfigure forSampling @08:29:19.99 SS.seek 25.00ml @08:29:20.71 Vacuum too high -- waiting up to 40s @08:29:21.18 Intake @47.4psia, vacuum from 20.0 to 24.0psi @08:29:21.19 SS.reconfigure forSampling @08:29:21.22 SS.seek 25.00ml @08:29:21.93 Vacuum too high -- waiting up to 40s @08:29:22.41 Intake @48.0psia, vacuum from 20.0 to 24.0psi @08:29:22.42 SS.reconfigure forSampling @08:29:22.46 SS.seek 25.00ml @08:29:23.12 Vacuum too high -- waiting up to 40s @08:29:23.60 Intake @48.4psia, vacuum from 20.0 to 24.0psi @08:29:23.61 SS.reconfigure forSampling @08:29:23.64 SS.seek 25.00ml @08:29:24.40 Vacuum too high -- waiting up to 40s @08:29:24.87 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:29:24.88 SS.reconfigure forSampling @08:29:24.91 SS.seek 25.00ml @08:29:25.71 Vacuum too high -- waiting up to 40s @08:29:26.18 Intake @47.3psia, vacuum from 20.0 to 24.0psi @08:29:26.19 SS.reconfigure forSampling @08:29:26.22 SS.seek 25.00ml @08:29:27.06 Vacuum too high -- waiting up to 40s @08:29:27.53 Intake @48.0psia, vacuum from 20.0 to 24.0psi @08:29:27.54 SS.reconfigure forSampling @08:29:27.57 SS.seek 25.00ml @08:29:28.28 Vacuum too high -- waiting up to 40s @08:29:28.75 Intake @48.1psia, vacuum from 20.0 to 24.0psi @08:29:28.76 SS.reconfigure forSampling @08:29:28.80 SS.seek 25.00ml @08:29:29.59 Vacuum too high -- waiting up to 40s @08:29:29.87 Intake @47.5psia, vacuum from 20.0 to 24.0psi @08:29:29.88 SS.reconfigure forSampling @08:29:29.91 SS.seek 25.00ml @08:29:30.46 Vacuum too high -- waiting up to 40s @08:29:30.93 Intake @47.2psia, vacuum from 20.0 to 24.0psi @08:29:30.94 SS.reconfigure forSampling @08:29:30.97 SS.seek 25.00ml @08:29:31.71 Vacuum too high -- waiting up to 40s @08:29:32.19 Intake @47.0psia, vacuum from 20.0 to 24.0psi @08:29:32.20 SS.reconfigure forSampling @08:29:32.23 SS.seek 25.00ml @08:29:33.00 Vacuum too high -- waiting up to 40s @08:29:33.57 Intake @47.3psia, vacuum from 20.0 to 24.0psi @08:29:33.58 SS.reconfigure forSampling @08:29:33.62 SS.seek 25.00ml @08:29:34.53 Vacuum too high -- waiting up to 40s @08:29:35.02 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:29:35.03 SS.reconfigure forSampling @08:29:35.06 SS.seek 25.00ml @08:29:35.87 Vacuum too high -- waiting up to 40s @08:29:36.34 Intake @48.0psia, vacuum from 20.0 to 24.0psi @08:29:36.35 SS.reconfigure forSampling @08:29:36.38 SS.seek 25.00ml @08:29:37.03 Vacuum too high -- waiting up to 40s @08:29:37.51 Intake @48.1psia, vacuum from 20.0 to 24.0psi @08:29:37.52 SS.reconfigure forSampling @08:29:37.55 SS.seek 25.00ml @08:29:38.34 Vacuum too high -- waiting up to 40s @08:29:38.81 Intake @48.0psia, vacuum from 20.0 to 24.0psi @08:29:38.82 SS.reconfigure forSampling @08:29:38.85 SS.seek 25.00ml @08:29:39.59 Vacuum too high -- waiting up to 40s @08:29:40.07 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:29:40.08 SS.reconfigure forSampling @08:29:40.11 SS.seek 25.00ml @08:29:40.74 Vacuum too high -- waiting up to 40s @08:29:41.21 Intake @47.4psia, vacuum from 20.0 to 24.0psi @08:29:41.22 SS.reconfigure forSampling @08:29:41.25 SS.seek 25.00ml @08:29:43.03 Vacuum too high -- waiting up to 40s @08:29:43.51 Intake @47.7psia, vacuum from 20.0 to 24.0psi @08:29:43.52 SS.reconfigure forSampling @08:29:43.55 SS.seek 25.00ml @08:29:44.34 Vacuum too high -- waiting up to 40s @08:29:44.82 Intake @47.5psia, vacuum from 20.0 to 24.0psi @08:29:44.83 SS.reconfigure forSampling @08:29:44.86 SS.seek 25.00ml @08:29:45.53 Vacuum too high -- waiting up to 40s @08:29:46.26 SS.seek 25.00ml @08:29:47.12 Vacuum too high -- waiting up to 40s @08:29:47.60 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:29:47.61 SS.reconfigure forSampling @08:29:47.65 SS.seek 25.00ml @08:29:48.28 Vacuum too high -- waiting up to 40s @08:29:48.76 SS.seek 25.00ml @08:29:49.40 Vacuum too high -- waiting up to 40s @08:29:49.88 Intake @47.7psia, vacuum from 20.0 to 24.0psi @08:29:49.89 SS.reconfigure forSampling @08:29:49.92 SS.seek 25.00ml @08:29:50.62 Vacuum too high -- waiting up to 40s @08:29:51.09 Intake @47.9psia, vacuum from 20.0 to 24.0psi @08:29:51.10 SS.reconfigure forSampling @08:29:51.13 SS.seek 25.00ml @08:29:51.96 Vacuum too high -- waiting up to 40s @08:29:52.44 Intake @48.0psia, vacuum from 20.0 to 24.0psi @08:29:52.45 SS.reconfigure forSampling @08:29:52.48 SS.seek 25.00ml @08:29:53.12 Vacuum too high -- waiting up to 40s @08:29:53.59 Intake @47.9psia, vacuum from 20.0 to 24.0psi @08:29:53.60 SS.reconfigure forSampling @08:29:53.63 SS.seek 25.00ml @08:29:54.40 Vacuum too high -- waiting up to 40s @08:29:54.88 Intake @47.5psia, vacuum from 20.0 to 24.0psi @08:29:54.89 SS.reconfigure forSampling @08:29:54.92 SS.seek 25.00ml @08:29:55.62 Vacuum too high -- waiting up to 40s @08:29:56.09 Intake @47.7psia, vacuum from 20.0 to 24.0psi @08:29:56.10 SS.reconfigure forSampling @08:29:56.13 SS.seek 25.00ml @08:29:56.90 Vacuum too high -- waiting up to 40s @08:29:57.37 Intake @48.3psia, vacuum from 20.0 to 24.0psi @08:29:57.38 SS.reconfigure forSampling @08:29:57.41 SS.seek 25.00ml @08:29:58.18 Vacuum too high -- waiting up to 40s @08:29:58.65 Intake @47.9psia, vacuum from 20.0 to 24.0psi @08:29:58.66 SS.reconfigure forSampling @08:29:58.70 SS.seek 25.00ml @08:29:59.46 Vacuum too high -- waiting up to 40s @08:29:59.93 Intake @47.3psia, vacuum from 20.0 to 24.0psi @08:29:59.94 SS.reconfigure forSampling @08:29:59.97 SS.seek 25.00ml @08:30:00.68 Vacuum too high -- waiting up to 40s @08:30:01.16 Intake @47.0psia, vacuum from 20.0 to 24.0psi @08:30:01.17 SS.reconfigure forSampling @08:30:01.20 SS.seek 25.00ml @08:30:01.96 Vacuum too high -- waiting up to 40s @08:30:02.43 Intake @47.2psia, vacuum from 20.0 to 24.0psi @08:30:02.44 SS.reconfigure forSampling @08:30:02.48 SS.seek 25.00ml @08:30:03.24 Vacuum too high -- waiting up to 40s @08:30:03.72 Intake @47.5psia, vacuum from 20.0 to 24.0psi @08:30:03.73 SS.reconfigure forSampling @08:30:03.76 SS.seek 25.00ml @08:30:04.34 Vacuum too high -- waiting up to 40s @08:30:04.81 Intake @48.1psia, vacuum from 20.0 to 24.0psi @08:30:04.82 SS.reconfigure forSampling @08:30:04.85 SS.seek 25.00ml @08:30:05.49 Vacuum too high -- waiting up to 40s @08:30:05.96 Intake @48.3psia, vacuum from 20.0 to 24.0psi @08:30:05.97 SS.reconfigure forSampling @08:30:06.00 SS.seek 25.00ml @08:30:06.53 SS.reconfigure SSconfig @08:30:06.56 SS.hold @08:30:06.72 Intake.select :close @08:30:07.42 ShallowSampler::Clogged in MAIN -- progress too slow after 195.5ml @08:30:07.43 SSV.dial :exhaust @08:30:08.22 Exhaust.select :open @08:30:08.32 SS.reconfigure SSexhale @08:30:08.36 SS.seek 0.00ml @08:30:16.22 Exhaust.select :close @08:30:16.32 Sampled 195.5ml @08:30:16.33 collectSample: Complete @08:30:16.34 Evacuating Puck (bubblePt=55.0psi, delay=15.0s) Intake.select :close @08:30:16.40 CSV.dial :waste @08:30:16.43 CS.seek 0.000ml @08:30:16.53 SSV.dial CBV,avoiding => exhaust @08:30:16.55
CBV.dial SSV @08:30:16.59 CSV.dial :air @08:30:17.43 CS.seek 10.000ml @08:30:18.56
CTV.dial :air @08:30:19.31 Sleepy.power :sampler,:OFF @08:30:19.37
delay 15 seconds @08:30:34.37 SSV.dial :spit,avoiding => exhaust @08:30:35.72 CTV.dial CSV,avoiding => intake @08:30:35.74
SS.seek 0.00ml @08:30:35.83 SSV.dial CBV,avoiding => exhaust @08:30:41.97 CBV.dial between CSV and waste2b @08:30:42.89 CTV.dial CSV,avoiding => air @08:30:42.91
CSV.dial CTV @08:30:43.35 CS.reconfigure CSpressurize @08:30:43.38 CS.seek 0.000ml @08:31:07.47 BubblePoint: Intake @70.2psia, Outlet @14.6psia @08:31:07.48 SS.reconfigure SSconfig @08:31:07.51 SS.seek 10.00ml @08:31:13.22 delay 15 seconds @08:31:28.32 Evacuated: Intake @69.4psia, Outlet @-0.0psia @08:31:28.33 SSV.dial :spit,avoiding => exhaust @08:31:29.65 SS.reconfigure SSexhale @08:31:29.68 SS.seek 0.00ml @08:31:34.92 SS.reconfigure SSfast @08:31:34.96 SS.seek 5.00ml @08:31:37.76 SSV.dial CBV,avoiding => exhaust @08:31:37.78
CBV.dial SSV @08:31:39.13 CTV.dial :air,avoiding => intake @08:31:40.43 CSV.dial CTV @08:31:40.44
SS.reconfigure SSconfig @08:31:40.48 SS.seek 10.00ml @08:31:41.44 CSV.dial :air @08:31:41.96 CS.reconfigure CSfast @08:31:41.99 CS.seek 0.000ml @08:31:43.58
SSV.dial :spit,avoiding => exhaust @08:31:44.36 CS.coast @08:31:44.93
SS.reconfigure SSexhale @08:31:44.96 SS.seek 0.00ml @08:31:50.15 SS.reconfigure SSfast @08:31:50.19 SS.seek 5.00ml @08:31:52.76 SSV.dial CBV,avoiding => exhaust @08:31:52.78
CBV.dial SSV @08:31:55.11 SSV.dial :spit,avoiding => exhaust @08:31:56.43 SS.reconfigure SSconfig @08:31:56.46 SS.seek 0.00ml @08:31:59.59 SS.coast @08:31:59.62 Evacuating Puck (bubblePt=55.0psi, delay=15.0s) Intake.select :close @08:31:59.68 CSV.dial :waste @08:31:59.77 SSV.dial CBV,avoiding => exhaust @08:31:59.79
CBV.dial SSV @08:32:00.44 CS.seek 0.000ml @08:32:00.48 CSV.dial :air @08:32:01.12
CTV.dial :air @08:32:01.13 delay 15 seconds @08:32:01.27 CS.seek 10.000ml @08:32:16.13
SSV.dial :spit,avoiding => exhaust @08:32:17.46 CTV.dial CSV,avoiding => intake @08:32:17.48
SS.reconfigure SSexhale @08:32:17.55 SS.seek 0.00ml @08:32:17.95 SSV.dial CBV,avoiding => exhaust @08:32:25.09 CBV.dial between CSV and waste2b @08:32:26.03 CSV.dial between air and CTV @08:32:26.33 CS.seek 7.272ml @08:32:33.49 CTV.dial CSV,avoiding => air @08:32:33.50
CSV.dial CTV @08:32:33.79 CS.reconfigure CSpressurize @08:32:33.82 CS.seek 0.000ml @08:32:52.82 CSV.dial :air @08:32:53.33 CS.reconfigure CSfast @08:32:53.36 CS.seek 10.000ml @08:33:16.89 CSV.dial between air and CTV @08:33:17.27 CS.seek 2.208ml @08:33:35.86 CTV.dial CSV,avoiding => air @08:33:35.87
CSV.dial CTV @08:33:36.16 CS.reconfigure CSpressurize @08:33:36.19 CS.seek 0.000ml @08:33:38.34 BubblePoint: Intake @73.6psia, Outlet @18.0psia @08:33:38.35 SS.reconfigure SSconfig @08:33:38.38 SS.seek 10.00ml @08:33:44.04 delay 15 seconds @08:33:59.69 Evacuated: Intake @72.7psia, Outlet @-0.2psia SSV.dial :spit,avoiding => exhaust @08:34:01.02 SS.reconfigure SSexhale @08:34:01.05 SS.seek 0.00ml @08:34:05.92 SS.reconfigure SSfast @08:34:05.99 SS.seek 5.00ml @08:34:08.89 SSV.dial CBV,avoiding => exhaust @08:34:08.91
CBV.dial SSV @08:34:10.27 CTV.dial :air,avoiding => intake @08:34:11.58 CSV.dial CTV @08:34:11.59
SS.reconfigure SSconfig @08:34:11.63 SS.seek 10.00ml @08:34:12.59 CSV.dial :air @08:34:13.09 CS.reconfigure CSfast @08:34:13.12 CS.seek 0.000ml @08:34:14.79
SSV.dial :spit,avoiding => exhaust @08:34:16.13 SS.reconfigure SSexhale @08:34:16.16 SS.seek 0.00ml @08:34:17.63 CS.coast @08:34:21.21
SS.reconfigure SSfast @08:34:21.25 SS.seek 5.00ml @08:34:24.05 SSV.dial CBV,avoiding => exhaust @08:34:24.07
CBV.dial SSV @08:34:26.38 SSV.dial :spit,avoiding => exhaust @08:34:27.70 SS.reconfigure SSconfig @08:34:27.73 SS.seek 0.00ml @08:34:30.90 SS.coast @08:34:30.93 Lysing: Adding 1.5ml lysis CBV.dial between CSV and waste1,avoiding => DBV @08:34:32.42 CTV.dial CSV @08:34:33.68 CSV.dial CSR @08:34:34.47 CSR.select :lysis @08:34:34.58 CS.reconfigure CSconfig @08:34:34.61 CS.seek 1.000ml @08:34:38.01 delay 5 seconds @08:34:43.03 CSR.select :air @08:34:43.17 CS.seek 6.000ml @08:34:56.63 delay 2 seconds @08:34:58.65 CSV.dial :waste1 @08:34:59.90 CS.reconfigure CSfast @08:34:59.93 CS.seek empty @08:35:14.32 CSV.dial CSR @08:35:15.58 CSR.select :lysis @08:35:15.68 CS.reconfigure CSconfig @08:35:15.72 CS.seek 1.500ml @08:35:20.38 delay 5 seconds @08:35:25.39 CSR.select :air @08:35:25.53 CS.seek 4.500ml @08:35:33.91 delay 2 seconds @08:35:35.93 CSV.dial CTV @08:35:36.43 CS.seek 1.275ml @08:35:45.42 Heating sh1 puck from tube 2 in Collection Clamp to 85.0C for 5:00 @08:35:45.43 CH.seek 85.0C,8:00 @08:38:26.46 CH.hold 5:00,85.0C @08:43:26.56 Collection Puck Heater at 85.0C after lysing @08:43:26.57 CS.seek 0.000ml @08:43:30.63 CTV.dial between spare and air,avoiding => intake @08:43:31.62 CSV.dial :air @08:43:32.08 CS.seek 5.000ml @08:43:45.55 CSV.dial CTV @08:43:46.07 delay 2 seconds @08:43:48.08 CSV.dial :air @08:43:48.59 delay 2 seconds @08:43:50.60 CSV.dial CTV @08:43:51.10 delay 2 seconds @08:43:53.11 CSV.dial :air @08:43:53.62 delay 2 seconds @08:43:55.63 CS.seek 0.000ml @08:44:09.15 Recovering Lysate into Collection syringe @08:44:09.16 CSV.dial CBV @08:44:10.16 CBV.dial CSV @08:44:10.52 CS.seek 4.999ml @08:44:24.05 delay 5 seconds @08:44:29.07 CTV.dial :air @08:44:29.55 CS.seek 6.999ml @08:44:35.49 delay 5 seconds @08:44:40.51 CSV.dial :air2 @08:44:40.94 CS.seek 10.000ml @08:44:49.39 CSV.dial between air and CTV,avoiding => CBV @08:44:50.53 Lysing Complete @08:44:50.58 Storing sh1 puck in Collection Clamp to tube 1 @08:44:50.71 SC.reconfigure SCtight @08:44:50.74 SC.seek 1 @08:44:53.02 SC.reconfigure SCloose @08:44:53.19 Elbow.reconfigure TightElbow @08:44:53.23 Elbow.seek 1 @08:44:54.84 Forearm.reconfigure TightForearm @08:44:54.87 Forearm.seek 1 + 800 counts @08:44:55.90 SE.reconfigure SEpick @08:44:55.95 SE.seek beamBreak @08:44:57.84 SE.configure SEconfig @08:44:57.96 Elbow.reconfigure LimpElbow @08:44:57.99 Forearm.reconfigure LooseForearm @08:44:58.03 Forearm.seek 1 @08:44:58.24 Elbow.hold @08:44:58.29 Elbow.reconfigure TightElbow @08:44:58.36 SE.seek 11.04 Pucks below Top Plate @08:44:58.77 Forearm.coast @08:44:58.80 Forearm.seek 1 + 342 counts @08:44:59.04 Forearm.reconfigure TightForearm @08:44:59.07 Forearm.seek 1 + 800 counts @08:44:59.32 SE.seek 12.04 Pucks below Top Plate @08:44:59.90 Forearm.reconfigure LooseForearm @08:44:59.93 Forearm.seek retract @08:45:00.85 Elbow.seek CC @08:45:02.38 Elbow.reconfigure LooseElbow @08:45:03.10 Forearm.seek CC @08:45:04.06 CC.seek sealed @08:45:04.99 Hand.grip @08:45:05.67 CC.seek open @08:45:09.03 Forearm.seek retract @08:45:10.02 Elbow.reconfigure TightElbow @08:45:10.06 Elbow.seek 1 @08:45:11.68 Elbow.reconfigure LimpElbow @08:45:11.72 Forearm.seek 1 @08:45:12.72 Elbow.hold @08:45:12.77 Elbow.reconfigure TightElbow @08:45:12.81 Hand.release @08:45:13.62 Forearm.coast @08:45:13.65 Forearm.seek 1 + 322 counts @08:45:13.88 Forearm.reconfigure TightForearm @08:45:13.91 Forearm.seek 1 + 800 counts @08:45:14.21 SE.seek down @08:45:15.31
Forearm.reconfigure LooseForearm @08:45:15.35 Forearm.seek retract @08:45:15.72 SE.coast @08:45:16.27
SC.coast @08:45:16.30 Tranferring lysate to PRV @08:45:16.31 CSV.dial :air @08:45:16.59 CS.seek 10.000ml @08:45:16.64 CSV.dial between air and CTV @08:45:17.22 AIV.dial :bypass @08:45:17.29 Beginning samplePassC @08:45:17.30 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:45:17.44 Elbow.seek FlushPuck::Garage @08:45:19.21 Elbow.reconfigure LooseElbow @08:45:19.29 Forearm.seek garage @08:45:20.34 Hand.grip @08:45:21.13 Forearm.seek retract @08:45:22.17 Elbow.reconfigure TightElbow @08:45:22.21 Elbow.seek CC @08:45:24.98 Elbow.reconfigure LooseElbow @08:45:25.07 Forearm.reconfigure TightForearm @08:45:25.10 Forearm.seek CC @08:45:26.23 CC.seek closed @08:45:30.01 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 2.03 @08:45:30.06 Hand.release @08:45:30.83 Forearm.reconfigure LooseForearm @08:45:30.86 Forearm.seek retract @08:45:31.84 AWV.select :air @08:45:31.85 AIV.dial :bypass @08:45:31.86 PSV.dial PSR @08:45:33.16 PSR.select :flush @08:45:33.26 PS.seek 1.500ml @08:45:49.28 delay 5 seconds @08:45:54.29 PSV.dial :waste1 @08:45:54.71 PS.seek 0.000ml @08:46:10.75 PSV.dial PSR @08:46:11.19 PSR.select :air @08:46:11.29 PS.seek 2.500ml @08:46:37.51 PRV.dial CBV @08:46:37.53
CBV.dial PRV @08:46:38.30 PSV.dial :mixloop @08:46:39.30 CTV.dial :air,avoiding => intake @08:46:39.31 PTV.dial :PRVmixing @08:46:39.38 PS.seek 0.000ml @08:47:05.65 PSV.dial :air @08:47:06.10 PS.seek 2.500ml @08:47:32.31 PSV.dial :mixloop @08:47:32.76 PS.seek 0.000ml @08:47:59.03 PSV.dial :air @08:47:59.47 PS.seek 2.500ml @08:48:25.68 PSV.dial :mixloop @08:48:26.13 PS.seek 0.000ml @08:48:52.37 Garaging FlushPuck in Collection Clamp @08:48:52.97 Elbow.reconfigure TightElbow @08:48:53.01 Elbow.seek CC @08:48:53.10 Elbow.reconfigure LooseElbow @08:48:53.18 Forearm.seek CC @08:48:54.13 CC.seek sealed @08:48:55.07 Hand.grip @08:48:55.78 CC.seek open @08:48:59.07 Forearm.seek retract @08:49:00.01 Elbow.reconfigure TightElbow @08:49:00.05 Elbow.seek garage @08:49:02.87 Elbow.reconfigure LooseElbow @08:49:02.96 Forearm.reconfigure TightForearm @08:49:02.99 Forearm.seek garage @08:49:04.16 Hand.release @08:49:04.95 Forearm.reconfigure LooseForearm @08:49:04.98 Forearm.seek retract @08:49:05.99 Connecting PS to CS to transfer sample @08:49:06.00 CBV.dial AIV @08:49:06.01 AIV.dial :bypass @08:49:06.02 PSV.dial :mixloop @08:49:06.03 PTV.dial :PRVmixing PRV.dial AIV @08:49:06.04 CTV.dial :air,avoiding => intake @08:49:06.05 Loading lyfil puck from tube 2 into Collection Clamp @08:49:06.23 SC.reconfigure SCtight @08:49:06.26 SC.seek 2 @08:49:08.57 SC.reconfigure SCloose @08:49:08.74 Elbow.reconfigure TightElbow @08:49:08.78 Elbow.seek 2 @08:49:10.60 Forearm.reconfigure TightForearm @08:49:10.63 Forearm.seek 2 + 800 counts @08:49:11.70 SE.reconfigure SEpick @08:49:11.75 SE.seek beamBreak @08:49:13.89 SE.configure SEconfig @08:49:14.01 Elbow.seek 2 @08:49:14.15 Forearm.seek 2 + 300 counts @08:49:14.41 Forearm.reconfigure LooseForearm @08:49:14.45 Hand.grip @08:49:15.17 SE.seek down @08:49:15.31
Forearm.seek retract @08:49:16.31 Elbow.seek CC @08:49:17.14 SE.coast @08:49:17.85
Elbow.reconfigure LooseElbow @08:49:17.93 Forearm.reconfigure TightForearm @08:49:17.96 Forearm.seek CC @08:49:19.06 CC.seek closed @08:49:22.81 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 2.02 @08:49:22.82 Hand.release @08:49:23.57 Forearm.reconfigure LooseForearm @08:49:23.60 Forearm.seek retract @08:49:24.63 SC.coast @08:49:24.66 CTV.dial CSV,avoiding => intake @08:49:25.96 CSV.dial CTV @08:49:26.33 CS.seek 5.000ml @08:49:39.76 delay 1:20 @08:50:59.77 CS.seek 2.500ml @08:51:06.91 delay 40 seconds @08:51:46.92 CS.seek 0.000ml @08:51:54.00 delay 1:00 @08:52:54.01 PSV.dial :air @08:52:54.46 PS.seek 2.500ml @08:53:20.73 PSV.dial :mixloop @08:53:21.17 delay 3 seconds @08:53:24.18 PSV.dial :air @08:53:24.61 delay 2 seconds @08:53:26.62 PSV.dial :mixloop @08:53:27.06 delay 3 seconds @08:53:30.07 PSV.dial :waste1 @08:53:31.35 PS.seek empty @08:53:57.61 CBV.dial :waste1 @08:53:58.07 delay 5 seconds @08:54:03.08 CBV.dial AIV @08:54:03.53 delay 10 seconds @08:54:13.54 CBV.dial :waste1 @08:54:14.01 delay 10 seconds @08:54:24.02 CBV.dial AIV @08:54:24.48 delay 10 seconds @08:54:34.49 CTV.dial CSV,avoiding => intake @08:54:34.50 CBV.dial between AIV and waste1 @08:54:34.82 CSV.dial :air @08:54:35.33 CS.seek 10.000ml @08:55:02.03 CSV.dial CTV @08:55:02.55 CS.seek 0.000ml @08:55:28.87 CBV.dial :waste1 @08:55:29.16 delay 10 seconds @08:55:39.17 CTV.dial :air,avoiding => intake @08:55:40.51 Storing lyfil puck in Collection Clamp to tube 1 @08:55:40.63 SC.reconfigure SCtight @08:55:40.66 SC.seek 1 @08:55:42.84 SC.reconfigure SCloose @08:55:43.02 Elbow.reconfigure TightElbow @08:55:43.05 Elbow.seek 1 @08:55:44.70 Forearm.reconfigure TightForearm @08:55:44.73 Forearm.seek 1 + 800 counts @08:55:45.70 SE.reconfigure SEpick @08:55:45.75 SE.seek beamBreak @08:55:47.58 SE.configure SEconfig @08:55:47.70 Elbow.reconfigure LimpElbow @08:55:47.73 Forearm.reconfigure LooseForearm @08:55:47.77 Forearm.seek 1 @08:55:47.99 Elbow.hold @08:55:48.04 Elbow.reconfigure TightElbow @08:55:48.12 SE.seek 12.05 Pucks below Top Plate @08:55:48.51 Forearm.coast @08:55:48.55 Forearm.seek 1 + 316 counts @08:55:48.79 Forearm.reconfigure TightForearm @08:55:48.82 Forearm.seek 1 + 800 counts @08:55:49.06 SE.seek 13.05 Pucks below Top Plate @08:55:49.83 Forearm.reconfigure LooseForearm @08:55:49.86 Forearm.seek retract @08:55:50.78 Elbow.seek CC @08:55:52.39 Elbow.reconfigure LooseElbow @08:55:52.48 Forearm.seek CC @08:55:53.44 CC.seek sealed @08:55:54.37 Hand.grip @08:55:55.14 CC.seek open @08:55:58.46 Forearm.seek retract @08:55:59.44 Elbow.reconfigure TightElbow @08:55:59.48 Elbow.seek 1 @08:56:01.08 Elbow.reconfigure LimpElbow @08:56:01.11 Forearm.seek 1 @08:56:02.13 Elbow.hold @08:56:02.18 Elbow.reconfigure TightElbow @08:56:02.22 Hand.release @08:56:03.09 Forearm.coast @08:56:03.12 Forearm.seek 1 + 306 counts @08:56:03.34 Forearm.reconfigure TightForearm @08:56:03.38 Forearm.seek 1 + 800 counts @08:56:03.69 SE.seek down @08:56:04.80
Forearm.reconfigure LooseForearm @08:56:04.83 Forearm.seek retract @08:56:05.12 SE.coast @08:56:05.77
SC.coast @08:56:05.80 PSV.dial :air @08:56:06.86 PS.seek 0.499ml @08:56:12.79 delay 3 seconds @08:56:15.81 PSV.dial :mixloop @08:56:16.24 PRV.dial :air @08:56:16.68 delay 3 seconds @08:56:19.69 PRV.dial CBV @08:56:20.13 CBV.dial AIV @08:56:20.58 PRV.dial between CBV and air @08:56:20.88 PRV.dial :air @08:56:21.23 PS.reconfigure PSslow @08:56:21.26 PS.seek 0.599ml @08:56:23.82 PRV.dial :waste2 @08:56:24.55 PS.reconfigure PSconfig @08:56:24.58 PS.seek empty @08:56:31.63 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:56:31.78 Elbow.seek FlushPuck::Garage @08:56:33.54 Elbow.reconfigure LooseElbow @08:56:33.63 Forearm.seek garage @08:56:34.64 Hand.grip @08:56:35.47 Forearm.seek retract @08:56:36.99 Elbow.reconfigure TightElbow @08:56:37.02 Elbow.seek CC @08:56:39.79 Elbow.reconfigure LooseElbow @08:56:39.87 Forearm.reconfigure TightForearm @08:56:39.90 Forearm.seek CC @08:56:41.04 CC.seek closed @08:56:44.80 Collection Clamp onSpring/offSpring for 3/21 samples, on/off torque 2.03 @08:56:44.85 Hand.release @08:56:45.68 Forearm.reconfigure LooseForearm @08:56:45.71 Forearm.seek retract @08:56:46.72 Clearing PML for modified lysate @08:56:46.73 CTV.dial :air,avoiding => intake @08:56:46.74 PRV.dial :waste2 @08:56:46.75 PTV.dial :PRV,avoiding => puck @08:56:47.47 delay 2 seconds @08:56:49.47 PTV.dial :PRVmixing,avoiding => puck @08:56:50.21 delay 2 seconds @08:56:52.23 PSV.dial :PML @08:56:52.24 delay 2 seconds @08:56:54.24 PRV.dial :air @08:56:54.95 delay 2 seconds @08:56:57.01 PS.seek 1.499ml @08:57:13.14 delay 5 seconds @08:57:18.15 PS.seek 2.500ml @08:57:29.08 PSV.dial :air @08:57:29.65 PS.seek 0.000ml @08:57:55.86 mixing lysate with speDil @08:57:55.87 PSV.dial :PML @08:57:56.30 CBV.dial :PRV @08:57:56.31 PRV.dial :air @08:57:56.32 delay 2 seconds @08:57:58.33 PRV.dial :speDil @08:57:58.78 delay 2 seconds @08:58:00.84 PS.reconfigure PSslow1 @08:58:00.87 PS.seek 0.225ml @08:58:24.14 delay 5 seconds @08:58:29.15 PRV.dial CBV @08:58:29.89 delay 2 seconds @08:58:31.95 PS.seek 0.475ml @08:58:57.92 delay 5 seconds @08:59:02.93 PRV.dial :air @08:59:03.37 delay 2 seconds @08:59:05.38 PS.seek 0.925ml @08:59:51.73 delay 10 seconds @09:00:01.74 PRV.dial CBV @09:00:02.18 CTV.dial :air,avoiding => intake @09:00:02.19 PSV.dial :air @09:00:02.65 PS.reconfigure PSconfig @09:00:02.68 PS.seek 2.500ml @09:00:19.57 delay 3 seconds @09:00:22.58 PSV.dial PTV @09:00:23.29 PTV.dial :PRV,avoiding => puck @09:00:24.02 PS.seek 0.000ml @09:00:50.26 PSV.dial :air @09:00:50.99 PS.seek 2.500ml @09:01:17.29 delay 3 seconds @09:01:20.36 PSV.dial PTV @09:01:21.08 PTV.dial :PRV,avoiding => puck @09:01:21.10 PS.seek 0.000ml @09:01:47.32 Pushing modified lysate into AIV Injection Loop @09:01:47.33 CBV.dial AIV @09:01:47.34 AIV.dial :bypass @09:01:47.35 PRV.dial CBV @09:01:47.36 PSV.dial :air @09:01:48.08 PS.seek 2.500ml @09:02:14.26 delay 3 seconds @09:02:17.27 PSV.dial :PML @09:02:17.71 PTV.dial :PRVmixing,avoiding => puck @09:02:18.48 PS.reconfigure PSslow1 @09:02:18.51 PS.seek 1.850ml @09:03:24.94 delay 5 seconds @09:03:29.95 AIV.dial :sample @09:03:30.48 AWV.select :waste @09:03:30.64 PS.reconfigure PSslow2 @09:03:30.67 PS.seek 1.310ml @09:05:20.75 delay 15 seconds @09:05:35.76 AIV.dial :bypass @09:05:36.31 PS.reconfigure PSconfig @09:05:36.34 PS.seek 0.000ml @09:05:50.53 PSV.dial :air @09:05:50.98 PS.seek 2.500ml @09:06:17.28 PSV.dial :PML @09:06:17.72 PS.seek 0.000ml @09:06:43.99 AIV.dial :coilToMFB @09:06:44.00 AWV.select :air @09:06:44.14 Extracting Nucleic Acid from AIV @09:06:44.16
Beginning samplePassD @09:06:44.17 SPE.hold 2:46:40,55.0C @09:06:44.19 ASV.dial :SPEdelivery @09:06:44.31
AWV.select :air @09:06:44.32 AIV.dial :bypass @09:06:44.33 PSV.dial PSR @09:06:45.02 delay 20 seconds @09:06:45.35
PSR.select :killMFB @09:06:46.02 PS.seek 1.500ml @09:07:02.06 delay 5 seconds @09:07:05.03 primeLysateINT @09:07:05.04 AWV.select :air @09:07:05.05 AIV.dial :coilToMFB @09:07:05.06 ATV.dial :AIV @09:07:06.40 AS.reconfigure ASslow1 @09:07:06.43 AS.seek 0.239ml @09:07:07.07
PSV.dial :waste1 @09:07:07.52 PS.seek 0.000ml @09:07:23.77 PSV.dial PSR @09:07:24.23 PSR.select :air @09:07:24.33 PS.seek 2.500ml @09:07:50.65 PRV.dial CBV @09:07:50.67
CBV.dial PRV @09:07:50.68 PSV.dial :mixloop @09:07:51.71 CTV.dial :air,avoiding => intake @09:07:51.72 PTV.dial :PRVmixing @09:07:51.74 PS.seek 0.000ml @09:07:55.67 delay 10 seconds @09:08:05.68 ATV.dial :air2 @09:08:06.26 AS.seek 0.269ml @09:08:12.61 ATV.dial :waste2 @09:08:12.97 AS.reconfigure ASnorm @09:08:13.00 AS.seek empty @09:08:18.15
PSV.dial PSR @09:08:19.18 PSR.select :flush @09:08:19.28 PS.seek 1.500ml @09:08:27.16 Lysate primed! @09:08:27.17 AFV.select :holdingCoil @09:08:27.18 ATV.dial :air2 @09:08:27.57 AS.reconfigure ASslow1 @09:08:27.60 AS.seek 0.069ml @09:08:35.30
delay 5 seconds @09:08:40.31 PSV.dial :waste1 @09:08:40.75 PS.seek 0.000ml @09:08:42.19 delay 3 seconds @09:08:45.20 ATV.dial :AIV @09:08:45.79 AS.seek 0.469ml @09:08:56.83
PSV.dial PSR @09:08:57.29 PSR.select :air @09:08:57.39 PS.seek 2.500ml @09:09:23.58 PRV.dial CBV @09:09:23.60
CBV.dial PRV @09:09:23.61 PSV.dial :mixloop @09:09:24.63 CTV.dial :air,avoiding => intake @09:09:24.64 PTV.dial :PRVmixing @09:09:24.66 PS.seek 0.000ml @09:09:50.93 PSV.dial PSR @09:09:52.07 PSR.select :flush @09:09:52.17 PS.seek 1.500ml @09:10:07.55 delay 5 seconds @09:10:08.30
delay 5 seconds @09:10:12.60 Solid Phase Extraction Heater at 51.7C @09:10:12.61 ATV.dial ASV @09:10:12.94 ASV.dial :SPEdelivery @09:10:13.00 AS.seek 0.041ml @09:10:13.31
PSV.dial :waste1 @09:10:13.78 PS.seek 0.000ml @09:10:29.92 PSV.dial PSR @09:10:30.38 PSR.select :air @09:10:30.48 PS.seek 2.500ml @09:10:56.67 PRV.dial CBV @09:10:56.69
CBV.dial PRV @09:10:56.70 PSV.dial :mixloop @09:10:57.70 CTV.dial :air,avoiding => intake @09:10:57.71 PTV.dial :PRVmixing @09:10:57.73 PS.seek 0.000ml @09:11:24.14 PSV.dial PSR @09:11:25.16 PSR.select :flush @09:11:25.26 PS.seek 1.500ml @09:11:40.53 delay 10 seconds @09:11:41.30
delay 5 seconds @09:11:46.31 PSV.dial :waste1 @09:11:46.74 PS.seek 0.000ml @09:11:50.54 rinseLysateLineINT @09:11:50.55 AFV.select :carrierSolution @09:11:50.64 delay 2 seconds @09:11:52.65 AS.reconfigure ASconfig @09:11:52.68 AS.seek 1.000ml @09:12:02.80
PSV.dial PSR @09:12:03.23 PSR.select :air @09:12:03.33 PS.seek 2.500ml @09:12:29.58 PRV.dial CBV @09:12:29.60
CBV.dial PRV @09:12:29.61 PSV.dial :mixloop @09:12:30.61 CTV.dial :air,avoiding => intake @09:12:30.62 PTV.dial :PRVmixing @09:12:30.63 PS.reconfigure PSslow @09:12:30.67 PS.seek 1.200ml @09:12:48.84 delay 1 second @09:12:49.85 AFV.select :holdingCoil @09:12:49.94 delay 1 second @09:12:50.95 ATV.dial :AIV @09:12:51.29 AIV.dial :coilToMFB @09:12:51.30 AWV.select :waste @09:12:51.39 AS.reconfigure ASslow0 @09:12:51.43 AS.seek 0.000ml @09:12:57.67
PSV.dial :waste1 @09:12:58.97 PS.reconfigure PSconfig @09:12:59.00 PS.seek 0.000ml @09:13:12.01 PSV.dial :air @09:13:13.04 PS.seek 2.500ml @09:13:39.32 PSV.dial :mixloop @09:13:39.77 PS.reconfigure PSslow @09:13:39.80 PS.seek 0.000ml @09:14:31.11 PSV.dial :air @09:14:31.56 PS.reconfigure PSconfig @09:14:31.59 PS.seek 2.500ml @09:14:33.78 ATV.dial :air2 @09:14:34.94 AS.reconfigure ASslow1 @09:14:34.98 AS.seek 0.050ml @09:14:45.47 ATV.dial :AIV @09:14:46.05 AS.seek 0.010ml @09:14:54.44 ATV.dial :waste @09:14:55.74 AS.seek empty @09:14:57.80
PSV.dial :mixloop @09:14:58.14 ethanol wash @09:14:58.15 ATV.dial :air2 @09:14:58.25
PS.reconfigure PSslow @09:14:58.28 PS.seek 0.000ml @09:14:59.10 AS.reconfigure ASslow2 @09:14:59.13 AS.seek 0.009ml @09:15:09.64 delay 3 seconds @09:15:12.65 Pulling 0.4ml of Ethanol Wash @09:15:12.66 ATV.dial :ethanol @09:15:13.24 AS.reconfigure ASslow1 @09:15:13.27 AS.seek 0.409ml @09:15:49.64
Cleaning Collection Syringe @09:15:49.65 CSV.dial CSR @09:15:50.08 CSR.select :flush @09:15:50.18 CS.seek 4.000ml @09:16:01.14 delay 5 seconds @09:16:06.15 CSR.select :air @09:16:06.24 CS.seek 10.000ml @09:16:22.33 CSV.dial CTV @09:16:22.35
CTV.dial CSV @09:16:23.69 CBV.dial :waste1 @09:16:24.17 CS.seek 0.000ml @09:16:35.11 delay 3 seconds @09:16:38.12 ATV.dial :air1 @09:16:38.45 delay 3 seconds @09:16:41.51 AS.seek 0.434ml @09:16:46.90 delay 3 seconds @09:16:49.91 ATV.dial :ethanol @09:16:50.30 AS.seek 0.419ml @09:16:50.52
CSV.dial CSR @09:16:51.02 CSR.select :flush @09:16:51.12 CS.seek 4.000ml @09:16:53.62 delay 3 seconds @09:16:56.63 ATV.dial ASV @09:16:57.87 AS.seek 0.092ml @09:17:02.02
delay 5 seconds @09:17:07.03 CSR.select :air @09:17:07.12 CS.seek 10.000ml @09:17:23.17 CSV.dial CTV @09:17:23.19
CTV.dial CSV @09:17:23.70 CBV.dial :waste1 @09:17:23.72 CS.seek 0.000ml @09:17:50.06 Garaging FlushPuck in Collection Clamp @09:17:50.11 Elbow.reconfigure TightElbow @09:17:50.14 Elbow.seek CC @09:17:50.23 Elbow.reconfigure LooseElbow @09:17:50.31 Forearm.seek CC @09:17:51.29 CC.seek sealed @09:17:52.23 Hand.grip @09:17:52.98 CC.seek open @09:17:56.29 Forearm.seek retract @09:17:57.28 Elbow.reconfigure TightElbow @09:17:57.32 Elbow.seek garage @09:18:00.13 Elbow.reconfigure LooseElbow @09:18:00.21 Forearm.reconfigure TightForearm @09:18:00.24 Forearm.seek garage @09:18:01.43 Hand.release @09:18:02.27 Forearm.reconfigure LooseForearm @09:18:02.30 Forearm.seek retract @09:18:03.34 Whole Cell Archive sampling 1000ml @09:18:03.36 Loading wcr puck from tube 2 into Collection Clamp @09:18:03.54 SC.reconfigure SCtight @09:18:03.57 SC.seek 2 @09:18:04.79 delay 3 seconds @09:18:05.92 SC.reconfigure SCloose @09:18:06.10 Elbow.reconfigure TightElbow @09:18:06.13 Elbow.seek 2 @09:18:07.80 ATV.dial :waste2 @09:18:07.96 Forearm.reconfigure TightForearm @09:18:07.99 Forearm.seek 2 + 800 counts @09:18:08.33 AS.reconfigure ASconfig @09:18:08.36 AS.seek empty @09:18:09.09 SE.reconfigure SEpick @09:18:09.14 SE.seek beamBreak @09:18:11.40 SE.configure SEconfig @09:18:11.52 Elbow.seek 2 @09:18:11.66 Forearm.seek 2 + 300 counts @09:18:11.86 Forearm.reconfigure LooseForearm @09:18:12.49 Hand.grip @09:18:13.25 SE.seek down @09:18:13.39 Forearm.seek retract @09:18:14.07 ATV.dial :air2 @09:18:14.35 Elbow.seek CC @09:18:14.41 AS.reconfigure ASslow1 @09:18:14.45 AS.seek 1.000ml @09:18:15.31 SE.coast @09:18:15.86 Elbow.reconfigure LooseElbow @09:18:15.95 Forearm.reconfigure TightForearm @09:18:15.98 Forearm.seek CC @09:18:17.00 CC.seek closed @09:18:20.78 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 2.01 @09:18:20.80 Hand.release @09:18:21.51 Forearm.reconfigure LooseForearm @09:18:21.54 Forearm.seek retract @09:18:22.53 SC.coast @09:18:22.56 collectSample 1000ml w/bubble point of 55psi @09:18:22.58 Priming 7ml Sleepy.power :sampler,:ON @09:18:22.59 Intake.select :close @09:18:22.60 Exhaust.select :close @09:18:22.61 SS.hold @09:18:22.77 Calibrating pressure sensors @09:18:22.78 CTV.dial :air @09:18:24.09 SSV.dial :spit,avoiding => exhaust @09:18:24.10 SS.reconfigure SSfast @09:18:24.13 SS.seek 5.00ml @09:18:26.85 SSV.dial CBV @09:18:26.86 CBV.dial SSV @09:18:28.29 delay 3.8 seconds @09:18:32.18 SSV.dial :spit @09:18:33.54 SS.seek 0.00ml @09:18:36.45 SSV.dial CBV @09:18:37.81 CTV.dial :intake @09:18:38.54 Waiting for external sampler to finish priming... @09:18:38.55 delayUntil "09:28:22.58" @09:21:38.81 delay 5 seconds @09:21:43.82 ATV.dial ASV @09:21:44.55 ASV.dial :SPEdelivery @09:21:44.57 AS.seek 0.020ml @09:25:04.73 delay 5 seconds @09:25:09.74 ATV.dial :air2 @09:25:10.53 AS.seek 0.520ml @09:26:52.76 delay 5 seconds @09:26:57.77 ATV.dial ASV @09:26:58.51 ASV.dial :wasteR @09:26:59.00 AS.seek 0.300ml @09:27:44.10 delay 20 seconds @09:28:04.11 AS.seek 0.070ml @09:28:22.58 SSV.dial CBV @09:28:22.60 CBV.dial SSV @09:28:22.61 Allowing 55s for 7.0ml of remaining 7.0ml @09:28:22.62 Intake.select :open @09:28:24.76 Intake @37.5psia, vacuum from 20.0 to 24.0psi @09:28:24.77 SS.reconfigure forSampling @09:28:24.80 SS.seek 7.00ml @09:28:30.15 Stroke completed -- waiting for pressure to equalize ... @09:28:30.79 Intake.select :close @09:28:30.92 SSV.dial :exhaust @09:28:31.73 Exhaust.select :open @09:28:31.83 SS.reconfigure SSexhale @09:28:31.87 SS.seek 0.00ml @09:28:35.70 Exhaust.select :close @09:28:35.81 Sampling 1000.0ml @09:28:35.82 Intake.select :close @09:28:35.83 Exhaust.select :close @09:28:35.84 SS.reconfigure SSconfig @09:28:35.87 SS.hold @09:28:36.03 Calibrating pressure and temperature sensors @09:28:36.05 CTV.dial :air @09:28:36.85 SSV.dial :spit,avoiding => exhaust @09:28:37.64 SS.reconfigure SSfast @09:28:37.67 SS.seek 5.00ml @09:28:40.51 SSV.dial CBV @09:28:40.53 CBV.dial SSV @09:28:41.96 delay 3.8 seconds @09:28:45.84 SSV.dial :spit @09:28:47.27 SS.seek 0.00ml @09:28:49.82 SSV.dial CBV @09:28:51.21 CTV.dial :intake @09:28:51.29 delay 20 seconds @09:28:52.03 CBV.dial SSV @09:28:52.04 SSV.dial CBV @09:28:52.06 Allowing 185s for 25.0ml of remaining 1000.0ml @09:28:52.07 Intake.select :open @09:28:54.20 Intake @37.5psia, vacuum from 20.0 to 24.0psi @09:28:54.81 SS.reconfigure forSampling @09:28:54.84 SS.seek 25.00ml @09:29:08.96 Stroke completed -- waiting for pressure to equalize ... @09:29:09.60 Intake.select :close @09:29:09.75 SSV.dial :exhaust @09:29:10.54 Exhaust.select :open @09:29:10.65 SS.reconfigure SSexhale @09:29:10.69 SS.seek 0.00ml @09:29:11.30 ATV.dial :waste2 @09:29:11.86 AS.reconfigure ASconfig @09:29:11.89 AS.seek empty @09:29:16.30 Elution @09:29:16.31 ATV.dial :air1 @09:29:17.32 AS.reconfigure ASslow1 @09:29:17.35 AS.seek 0.049ml @09:29:19.88 Exhaust.select :close @09:29:19.98 SSV.dial CBV @09:29:20.00 CBV.dial SSV @09:29:20.82 Allowing 185s for 25.0ml of remaining 975.0ml @09:29:20.84 Intake.select :open @09:29:22.97 Intake @36.3psia, vacuum from 20.0 to 24.0psi @09:29:22.99 SS.reconfigure forSampling @09:29:23.02 SS.seek 25.00ml @09:29:27.85 delay 3 seconds @09:29:30.86 ATV.dial :ElutionWater @09:29:31.46 AS.seek 0.109ml @09:29:32.68 Vacuum too high -- waiting up to 40s @09:29:32.95 Intake @28.3psia, vacuum from 20.0 to 24.0psi @09:29:32.97 SS.reconfigure forSampling @09:29:33.00 SS.seek 25.00ml @09:29:38.38 Stroke completed -- waiting for pressure to equalize ... @09:29:39.02 Intake.select :close @09:29:39.16 SSV.dial :exhaust @09:29:39.96 Exhaust.select :open @09:29:40.06 SS.reconfigure SSexhale @09:29:40.10 SS.seek 0.00ml @09:29:43.97 delay 3 seconds @09:29:46.98 ATV.dial ASV,via => air2 @09:29:48.75 ASV.dial :SPEelution @09:29:49.40 Exhaust.select :close @09:29:49.50 SSV.dial CBV @09:29:49.52 CBV.dial SSV @09:29:49.72 AFV.select :carrierSolution @09:29:49.87 AS.reconfigure ASnorm @09:29:49.90 AS.seek 0.759ml @09:29:50.34 Allowing 185s for 25.0ml of remaining 950.0ml @09:29:50.35 Intake.select :open @09:29:52.48 Intake @36.0psia, vacuum from 20.0 to 24.0psi @09:29:52.50 SS.reconfigure forSampling @09:29:52.53 SS.seek 25.00ml @09:29:56.30 Vacuum too high -- waiting up to 40s @09:29:56.57 Intake @29.5psia, vacuum from 20.0 to 24.0psi @09:29:56.59 SS.reconfigure forSampling @09:29:56.62 SS.seek 25.00ml @09:30:05.17 Vacuum too high -- waiting up to 40s @09:30:05.86 Intake @33.1psia, vacuum from 20.0 to 24.0psi @09:30:05.87 SS.reconfigure forSampling @09:30:05.91 SS.seek 25.00ml @09:30:08.81 Vacuum too high -- waiting up to 40s @09:30:09.28 Intake @33.2psia, vacuum from 20.0 to 24.0psi @09:30:09.30 SS.reconfigure forSampling @09:30:09.33 SS.seek 25.00ml @09:30:12.45 Vacuum too high -- waiting up to 40s @09:30:12.94 Intake @33.7psia, vacuum from 20.0 to 24.0psi @09:30:12.95 SS.reconfigure forSampling @09:30:12.99 SS.seek 25.00ml @09:30:13.21 Stroke completed -- waiting for pressure to equalize ... @09:30:13.85 Intake.select :close @09:30:13.98 SSV.dial :exhaust @09:30:14.80 Exhaust.select :open @09:30:14.91 SS.reconfigure SSexhale @09:30:14.95 SS.seek 0.00ml @09:30:23.41 delay 3 seconds @09:30:23.86 Exhaust.select :close @09:30:23.96 SSV.dial CBV @09:30:23.98 CBV.dial SSV @09:30:24.83 Allowing 185s for 25.0ml of remaining 925.0ml @09:30:24.84 Intake.select :open @09:30:26.42 AFV.select :holdingCoil @09:30:26.56 AS.reconfigure ASslow1 @09:30:26.59 AS.seek 0.489ml @09:30:26.97 Intake @36.7psia, vacuum from 20.0 to 24.0psi @09:30:26.99 SS.reconfigure forSampling @09:30:27.02 SS.seek 25.00ml @09:30:30.11 Vacuum too high -- waiting up to 40s @09:30:30.38 Intake @33.2psia, vacuum from 20.0 to 24.0psi @09:30:30.39 SS.reconfigure forSampling @09:30:30.43 SS.seek 25.00ml @09:30:44.23 Vacuum too high -- waiting up to 40s @09:30:44.72 Intake @34.4psia, vacuum from 20.0 to 24.0psi @09:30:44.73 SS.reconfigure forSampling @09:30:44.77 SS.seek 25.00ml @09:30:59.81 Vacuum too high -- waiting up to 40s @09:31:00.88 Intake @36.5psia, vacuum from 20.0 to 24.0psi @09:31:00.89 SS.reconfigure forSampling @09:31:00.92 SS.seek 25.00ml @09:31:06.23 Stroke completed -- waiting for pressure to equalize ... @09:31:07.46 Intake.select :close @09:31:07.60 SSV.dial :exhaust @09:31:08.42 Exhaust.select :open @09:31:08.52 SS.reconfigure SSexhale @09:31:08.56 SS.seek 0.00ml @09:31:17.26 Exhaust.select :close @09:31:17.36 SSV.dial CBV @09:31:17.38 CBV.dial SSV @09:31:18.20 Allowing 185s for 25.0ml of remaining 900.0ml @09:31:18.21 Intake.select :open @09:31:20.35 Intake @36.1psia, vacuum from 20.0 to 24.0psi @09:31:20.37 SS.reconfigure forSampling @09:31:20.40 SS.seek 25.00ml @09:31:21.93 delay 5 seconds @09:31:26.99 AS.reconfigure ASslow2 @09:31:27.02 AS.seek 0.454ml @09:32:02.93 delay 1:00 @09:32:07.15 Vacuum too high -- waiting up to 40s @09:32:07.43 Intake @36.1psia, vacuum from 20.0 to 24.0psi @09:32:07.45 SS.reconfigure forSampling @09:32:07.48 SS.seek 25.00ml @09:32:31.24 Stroke completed -- waiting for pressure to equalize ... @09:32:32.47 Intake.select :close @09:32:32.61 SSV.dial :exhaust @09:32:33.41 Exhaust.select :open @09:32:33.51 SS.reconfigure SSexhale @09:32:33.55 SS.seek 0.00ml @09:32:42.41 Exhaust.select :close @09:32:42.51 SSV.dial CBV @09:32:42.53 CBV.dial SSV @09:32:43.35 Allowing 185s for 25.0ml of remaining 875.0ml @09:32:43.36 Intake.select :open @09:32:45.50 Intake @36.7psia, vacuum from 20.0 to 24.0psi @09:32:45.52 SS.reconfigure forSampling @09:32:45.55 SS.seek 25.00ml @09:33:02.94 pushing eluate to holding coil @09:33:03.00 AS.seek 0.294ml @09:34:36.05 Stroke completed -- waiting for pressure to equalize ... @09:34:37.85 Intake.select :close @09:34:37.98 SSV.dial :exhaust @09:34:38.78 Exhaust.select :open @09:34:38.88 SS.reconfigure SSexhale @09:34:38.92 SS.seek 0.00ml @09:34:47.64 Exhaust.select :close @09:34:47.74 SSV.dial CBV @09:34:47.76 CBV.dial SSV @09:34:48.58 Allowing 185s for 25.0ml of remaining 850.0ml @09:34:48.59 Intake.select :open @09:34:50.73 Intake @37.4psia, vacuum from 20.0 to 24.0psi @09:34:50.75 SS.reconfigure forSampling @09:34:50.78 SS.seek 25.00ml @09:35:46.30 delay 30 seconds @09:36:14.55 Vacuum too high -- waiting up to 40s @09:36:15.03 Intake @36.6psia, vacuum from 20.0 to 24.0psi @09:36:15.04 SS.reconfigure forSampling @09:36:15.08 SS.seek 25.00ml @09:36:16.31 ATV.dial :waste,via => air3 @09:36:17.84 AS.reconfigure ASconfig @09:36:17.87 AS.seek empty @09:36:29.69 Vacuum too high -- waiting up to 40s @09:36:30.17 Intake @36.7psia, vacuum from 20.0 to 24.0psi @09:36:30.18 SS.reconfigure forSampling @09:36:30.22 SS.seek 25.00ml @09:36:35.36 Parking Bleach in LysateSource line and SPEcolumn @09:36:35.37 ATV.dial :air3 @09:36:35.95 AS.reconfigure ASslow1 @09:36:35.98 AS.seek 0.029ml @09:36:36.42 Vacuum too high -- waiting up to 40s @09:36:36.91 Intake @36.9psia, vacuum from 20.0 to 24.0psi @09:36:36.92 SS.reconfigure forSampling @09:36:36.96 SS.seek 25.00ml @09:36:41.66 Vacuum too high -- waiting up to 40s @09:36:42.15 Intake @36.6psia, vacuum from 20.0 to 24.0psi @09:36:42.16 SS.reconfigure forSampling @09:36:42.20 SS.seek 25.00ml @09:36:42.40 ATV.dial :ElutionWater @09:36:42.80 AS.seek 0.059ml @09:36:44.32 Stroke completed -- waiting for pressure to equalize ... @09:36:46.71 Intake.select :close @09:36:46.84 SSV.dial :exhaust @09:36:47.65 Exhaust.select :open @09:36:47.75 SS.reconfigure SSexhale @09:36:47.79 SS.seek 0.00ml @09:36:49.22 ATV.dial :air3 @09:36:49.62 AS.seek 0.069ml @09:36:52.00 delay 2 seconds @09:36:54.01 ATV.dial :bleach @09:36:54.41 AS.seek 0.709ml @09:36:57.49 Exhaust.select :close @09:36:57.59 SSV.dial CBV @09:36:57.61 CBV.dial SSV @09:36:58.44 Allowing 185s for 25.0ml of remaining 825.0ml @09:36:58.45 Intake.select :open @09:37:00.58 Intake @37.8psia, vacuum from 20.0 to 24.0psi @09:37:00.60 SS.reconfigure forSampling @09:37:00.63 SS.seek 25.00ml @09:37:05.95 Vacuum too high -- waiting up to 40s @09:37:06.44 Intake @36.7psia, vacuum from 20.0 to 24.0psi @09:37:06.45 SS.reconfigure forSampling @09:37:06.48 SS.seek 25.00ml @09:37:12.63 Vacuum too high -- waiting up to 40s @09:37:13.12 Intake @37.0psia, vacuum from 20.0 to 24.0psi @09:37:13.13 SS.reconfigure forSampling @09:37:13.17 SS.seek 25.00ml @09:37:16.79 Vacuum too high -- waiting up to 40s @09:37:17.28 Intake @36.9psia, vacuum from 20.0 to 24.0psi @09:37:17.29 SS.reconfigure forSampling @09:37:17.33 SS.seek 25.00ml @09:37:22.22 Vacuum too high -- waiting up to 40s @09:37:22.71 Intake @37.2psia, vacuum from 20.0 to 24.0psi @09:37:22.72 SS.reconfigure forSampling @09:37:22.75 SS.seek 25.00ml @09:37:25.94 Vacuum too high -- waiting up to 40s @09:37:26.43 Intake @36.9psia, vacuum from 20.0 to 24.0psi @09:37:26.44 SS.reconfigure forSampling @09:37:26.48 SS.seek 25.00ml @09:37:29.78 Vacuum too high -- waiting up to 40s @09:37:30.27 Intake @36.8psia, vacuum from 20.0 to 24.0psi @09:37:30.28 SS.reconfigure forSampling @09:37:30.32 SS.seek 25.00ml @09:37:34.10 Vacuum too high -- waiting up to 40s @09:37:34.80 Intake @37.9psia, vacuum from 20.0 to 24.0psi @09:37:34.82 SS.reconfigure forSampling @09:37:34.85 SS.seek 25.00ml @09:37:37.88 Vacuum too high -- waiting up to 40s @09:37:38.36 Intake @37.3psia, vacuum from 20.0 to 24.0psi @09:37:38.38 SS.reconfigure forSampling @09:37:38.41 SS.seek 25.00ml @09:37:41.23 Vacuum too high -- waiting up to 40s @09:37:41.72 Intake @36.8psia, vacuum from 20.0 to 24.0psi @09:37:41.73 SS.reconfigure forSampling @09:37:41.77 SS.seek 25.00ml @09:37:44.85 Vacuum too high -- waiting up to 40s @09:37:45.56 Intake @37.0psia, vacuum from 20.0 to 24.0psi @09:37:45.57 SS.reconfigure forSampling @09:37:45.61 SS.seek 25.00ml @09:37:49.06 Vacuum too high -- waiting up to 40s @09:37:49.76 Intake @38.2psia, vacuum from 20.0 to 24.0psi @09:37:49.77 SS.reconfigure forSampling @09:37:49.81 SS.seek 25.00ml @09:37:52.17 Vacuum too high -- waiting up to 40s @09:37:52.65 Intake @37.0psia, vacuum from 20.0 to 24.0psi @09:37:52.67 SS.reconfigure forSampling @09:37:52.70 SS.seek 25.00ml @09:37:55.48 Vacuum too high -- waiting up to 40s @09:37:55.97 Intake @37.1psia, vacuum from 20.0 to 24.0psi @09:37:55.98 SS.reconfigure forSampling @09:37:56.02 SS.seek 25.00ml @09:37:58.39 Vacuum too high -- waiting up to 40s @09:37:58.86 Intake @37.0psia, vacuum from 20.0 to 24.0psi @09:37:58.87 SS.reconfigure forSampling @09:37:58.91 SS.seek 25.00ml @09:38:01.23 Vacuum too high -- waiting up to 40s @09:38:01.71 Intake @36.8psia, vacuum from 20.0 to 24.0psi @09:38:01.72 SS.reconfigure forSampling @09:38:01.76 SS.seek 25.00ml @09:38:05.92 Vacuum too high -- waiting up to 40s @09:38:06.62 Intake @38.0psia, vacuum from 20.0 to 24.0psi @09:38:06.64 SS.reconfigure forSampling @09:38:06.67 SS.seek 25.00ml @09:38:08.82 Vacuum too high -- waiting up to 40s @09:38:09.31 Intake @37.4psia, vacuum from 20.0 to 24.0psi @09:38:09.33 SS.reconfigure forSampling @09:38:09.36 SS.seek 25.00ml @09:38:11.63 Vacuum too high -- waiting up to 40s @09:38:12.13 SS.seek 25.00ml @09:38:14.13 Vacuum too high -- waiting up to 40s @09:38:14.62 Intake @37.0psia, vacuum from 20.0 to 24.0psi @09:38:14.64 SS.reconfigure forSampling @09:38:14.67 SS.seek 25.00ml @09:38:17.07 Vacuum too high -- waiting up to 40s @09:38:17.79 Intake @37.0psia, vacuum from 20.0 to 24.0psi @09:38:17.80 SS.reconfigure forSampling @09:38:17.84 SS.seek 25.00ml @09:38:20.42 Vacuum too high -- waiting up to 40s @09:38:21.12 Intake @37.9psia, vacuum from 20.0 to 24.0psi @09:38:21.13 SS.reconfigure forSampling @09:38:21.17 SS.seek 25.00ml @09:38:23.29 Vacuum too high -- waiting up to 40s @09:38:23.78 SS.seek 25.00ml @09:38:25.67 Vacuum too high -- waiting up to 40s @09:38:26.15 Intake @37.0psia, vacuum from 20.0 to 24.0psi @09:38:26.17 SS.reconfigure forSampling @09:38:26.20 SS.seek 25.00ml @09:38:28.45 Vacuum too high -- waiting up to 40s @09:38:29.16 Intake @37.2psia, vacuum from 20.0 to 24.0psi @09:38:29.18 SS.reconfigure forSampling @09:38:29.21 SS.seek 25.00ml @09:38:31.57 Vacuum too high -- waiting up to 40s @09:38:32.06 Intake @37.3psia, vacuum from 20.0 to 24.0psi @09:38:32.07 SS.reconfigure forSampling @09:38:32.11 SS.seek 25.00ml @09:38:34.07 Vacuum too high -- waiting up to 40s @09:38:34.56 Intake @37.9psia, vacuum from 20.0 to 24.0psi @09:38:34.58 SS.reconfigure forSampling @09:38:34.61 SS.seek 25.00ml @09:38:36.45 Vacuum too high -- waiting up to 40s @09:38:36.93 Intake @37.6psia, vacuum from 20.0 to 24.0psi @09:38:36.95 SS.reconfigure forSampling @09:38:36.98 SS.seek 25.00ml @09:38:38.88 Vacuum too high -- waiting up to 40s @09:38:39.36 Intake @37.3psia, vacuum from 20.0 to 24.0psi @09:38:39.37 SS.reconfigure forSampling @09:38:39.41 SS.seek 25.00ml @09:38:41.20 Vacuum too high -- waiting up to 40s @09:38:41.93 Intake @37.6psia, vacuum from 20.0 to 24.0psi @09:38:41.94 SS.reconfigure forSampling @09:38:41.98 SS.seek 25.00ml @09:38:44.29 Vacuum too high -- waiting up to 40s @09:38:44.78 Intake @37.4psia, vacuum from 20.0 to 24.0psi @09:38:44.79 SS.reconfigure forSampling @09:38:44.83 SS.seek 25.00ml @09:38:46.60 Vacuum too high -- waiting up to 40s @09:38:47.08 Intake @37.1psia, vacuum from 20.0 to 24.0psi @09:38:47.09 SS.reconfigure forSampling @09:38:47.13 SS.seek 25.00ml @09:38:49.07 Vacuum too high -- waiting up to 40s @09:38:49.76 Intake @37.2psia, vacuum from 20.0 to 24.0psi @09:38:50.43 SS.reconfigure forSampling @09:38:50.46 SS.seek 25.00ml @09:38:53.56 Vacuum too high -- waiting up to 40s @09:38:54.28 Intake @37.5psia, vacuum from 20.0 to 24.0psi @09:38:54.29 SS.reconfigure forSampling @09:38:54.32 SS.seek 25.00ml @09:38:56.32 Vacuum too high -- waiting up to 40s @09:38:56.82 Intake @37.1psia, vacuum from 20.0 to 24.0psi @09:38:56.84 SS.reconfigure forSampling @09:38:56.87 SS.seek 25.00ml @09:38:58.79 Vacuum too high -- waiting up to 40s @09:38:59.28 Intake @37.2psia, vacuum from 20.0 to 24.0psi @09:38:59.30 SS.reconfigure forSampling @09:38:59.33 SS.seek 25.00ml @09:39:01.07 Vacuum too high -- waiting up to 40s @09:39:01.79 SS.seek 25.00ml @09:39:03.76 Vacuum too high -- waiting up to 40s @09:39:04.25 Intake @37.0psia, vacuum from 20.0 to 24.0psi @09:39:04.27 SS.reconfigure forSampling @09:39:04.30 SS.seek 25.00ml @09:39:05.26 delay 3 seconds @09:39:06.10 Vacuum too high -- waiting up to 40s @09:39:06.81 Intake @38.5psia, vacuum from 20.0 to 24.0psi @09:39:06.83 SS.reconfigure forSampling @09:39:06.86 SS.seek 25.00ml @09:39:08.27 ATV.dial :air3 @09:39:08.38 Vacuum too high -- waiting up to 40s @09:39:08.72 delay 3 seconds @09:39:08.87 Intake @37.3psia, vacuum from 20.0 to 24.0psi @09:39:08.89 SS.reconfigure forSampling @09:39:08.92 SS.seek 25.00ml @09:39:10.91 Vacuum too high -- waiting up to 40s @09:39:11.61 Intake @37.7psia, vacuum from 20.0 to 24.0psi @09:39:11.63 SS.reconfigure forSampling @09:39:11.66 SS.seek 25.00ml @09:39:11.77 AS.seek 0.734ml @09:39:13.54 Vacuum too high -- waiting up to 40s @09:39:14.02 Intake @37.1psia, vacuum from 20.0 to 24.0psi @09:39:14.04 SS.reconfigure forSampling @09:39:14.07 SS.seek 25.00ml @09:39:15.88 Vacuum too high -- waiting up to 40s @09:39:16.59 Intake @37.0psia, vacuum from 20.0 to 24.0psi @09:39:16.60 SS.reconfigure forSampling @09:39:16.64 SS.seek 25.00ml @09:39:17.15 delay 3 seconds @09:39:19.01 Vacuum too high -- waiting up to 40s @09:39:19.73 Intake @38.1psia, vacuum from 20.0 to 24.0psi @09:39:19.74 SS.reconfigure forSampling @09:39:19.77 SS.seek 25.00ml @09:39:20.16 ATV.dial :bleach @09:39:20.56 AS.seek 0.719ml @09:39:21.60 Vacuum too high -- waiting up to 40s @09:39:22.09 Intake @37.7psia, vacuum from 20.0 to 24.0psi @09:39:22.11 SS.reconfigure forSampling @09:39:22.14 SS.seek 25.00ml @09:39:23.76 Vacuum too high -- waiting up to 40s @09:39:23.84 delay 3 seconds @09:39:24.47 Intake @37.7psia, vacuum from 20.0 to 24.0psi @09:39:24.48 SS.reconfigure forSampling @09:39:24.51 SS.seek 25.00ml @09:39:26.51 Vacuum too high -- waiting up to 40s @09:39:26.86 ATV.dial :AIV @09:39:27.02 Intake @37.4psia, vacuum from 20.0 to 24.0psi @09:39:27.03 SS.reconfigure forSampling @09:39:27.07 SS.seek 25.00ml @09:39:28.07 AS.reconfigure ASconfig @09:39:28.10 AS.seek 0.015ml @09:39:28.66 Vacuum too high -- waiting up to 40s @09:39:30.04 Intake @37.2psia, vacuum from 20.0 to 24.0psi @09:39:30.06 SS.reconfigure forSampling @09:39:30.09 SS.seek 25.00ml @09:39:32.41 Stroke completed -- waiting for pressure to equalize ... @09:39:35.41 Intake.select :close @09:39:35.54 SSV.dial :exhaust @09:39:36.34 Exhaust.select :open @09:39:36.44 SS.reconfigure SSexhale @09:39:36.48 SS.seek 0.00ml @09:39:45.59 Exhaust.select :close @09:39:45.69 SSV.dial CBV @09:39:45.71 CBV.dial SSV @09:39:46.53 Allowing 185s for 25.0ml of remaining 800.0ml @09:39:46.55 Intake.select :open @09:39:48.68 Intake @37.7psia, vacuum from 20.0 to 24.0psi @09:39:48.70 SS.reconfigure forSampling @09:39:48.73 SS.seek 25.00ml @09:39:52.00 Vacuum too high -- waiting up to 40s @09:39:52.49 Intake @37.2psia, vacuum from 20.0 to 24.0psi @09:39:52.50 SS.reconfigure forSampling @09:39:52.54 SS.seek 25.00ml @09:39:54.38 Vacuum too high -- waiting up to 40s @09:39:55.09 Intake @37.4psia, vacuum from 20.0 to 24.0psi @09:39:55.11 SS.reconfigure forSampling @09:39:55.14 SS.seek 25.00ml @09:39:57.22 Vacuum too high -- waiting up to 40s @09:39:57.93 Intake @37.4psia, vacuum from 20.0 to 24.0psi @09:39:57.95 SS.reconfigure forSampling @09:39:57.98 SS.seek 25.00ml @09:39:59.94 Vacuum too high -- waiting up to 40s @09:40:00.65 Intake @37.9psia, vacuum from 20.0 to 24.0psi @09:40:00.67 SS.reconfigure forSampling @09:40:00.70 SS.seek 25.00ml @09:40:02.47 Vacuum too high -- waiting up to 40s @09:40:02.96 Intake @37.7psia, vacuum from 20.0 to 24.0psi @09:40:02.97 SS.reconfigure forSampling @09:40:03.01 SS.seek 25.00ml @09:40:04.72 Vacuum too high -- waiting up to 40s @09:40:05.43 Intake @38.3psia, vacuum from 20.0 to 24.0psi @09:40:05.44 SS.reconfigure forSampling @09:40:05.48 SS.seek 25.00ml @09:40:07.22 Vacuum too high -- waiting up to 40s @09:40:07.70 Intake @37.3psia, vacuum from 20.0 to 24.0psi @09:40:07.71 SS.reconfigure forSampling @09:40:07.74 SS.seek 25.00ml @09:40:09.43 ATV.dial :waste @09:40:09.49 Vacuum too high -- waiting up to 40s @09:40:10.20 Intake @37.4psia, vacuum from 20.0 to 24.0psi @09:40:10.22 SS.reconfigure forSampling @09:40:10.25 SS.seek 25.00ml @09:40:10.88 AS.seek empty @09:40:12.07 ATV.dial :air3 @09:40:12.22 Vacuum too high -- waiting up to 40s @09:40:12.78 AS.reconfigure ASslow1 @09:40:12.81 Intake @37.0psia, vacuum from 20.0 to 24.0psi @09:40:12.83 SS.reconfigure forSampling @09:40:12.86 AS.seek 0.009ml @09:40:12.92 SS.seek 25.00ml @09:40:14.88 Vacuum too high -- waiting up to 40s @09:40:15.24 delay 2 seconds @09:40:15.61 Intake @38.1psia, vacuum from 20.0 to 24.0psi @09:40:15.63 SS.reconfigure forSampling @09:40:15.66 SS.seek 25.00ml @09:40:17.25 ATV.dial :bleach @09:40:17.50 Vacuum too high -- waiting up to 40s @09:40:17.64 AS.seek 0.579ml @09:40:18.02 Intake @37.3psia, vacuum from 20.0 to 24.0psi @09:40:18.03 SS.reconfigure forSampling @09:40:18.06 SS.seek 25.00ml @09:40:19.85 Vacuum too high -- waiting up to 40s @09:40:20.75 Intake @37.2psia, vacuum from 20.0 to 24.0psi @09:40:20.77 SS.reconfigure forSampling @09:40:20.80 SS.seek 25.00ml @09:40:22.88 Vacuum too high -- waiting up to 40s @09:40:23.60 Intake @37.6psia, vacuum from 20.0 to 24.0psi @09:40:23.61 SS.reconfigure forSampling @09:40:23.64 SS.seek 25.00ml @09:40:25.44 Vacuum too high -- waiting up to 40s @09:40:26.16 Intake @37.7psia, vacuum from 20.0 to 24.0psi @09:40:26.17 SS.reconfigure forSampling @09:40:26.20 SS.seek 25.00ml @09:40:28.10 Vacuum too high -- waiting up to 40s @09:40:28.59 Intake @37.6psia, vacuum from 20.0 to 24.0psi @09:40:28.61 SS.reconfigure forSampling @09:40:28.64 SS.seek 25.00ml @09:40:30.10 Vacuum too high -- waiting up to 40s @09:40:30.81 Intake @38.1psia, vacuum from 20.0 to 24.0psi @09:40:30.83 SS.reconfigure forSampling @09:40:30.86 SS.seek 25.00ml @09:40:32.56 Vacuum too high -- waiting up to 40s @09:40:33.27 Intake @37.7psia, vacuum from 20.0 to 24.0psi @09:40:33.29 SS.reconfigure forSampling @09:40:33.32 SS.seek 25.00ml @09:40:35.10 Vacuum too high -- waiting up to 40s @09:40:35.59 Intake @37.5psia, vacuum from 20.0 to 24.0psi @09:40:35.60 SS.reconfigure forSampling @09:40:35.64 SS.seek 25.00ml @09:40:37.16 Vacuum too high -- waiting up to 40s @09:40:37.87 Intake @37.6psia, vacuum from 20.0 to 24.0psi @09:40:37.89 SS.reconfigure forSampling @09:40:37.92 SS.seek 25.00ml @09:40:39.72 Vacuum too high -- waiting up to 40s @09:40:40.43 Intake @37.7psia, vacuum from 20.0 to 24.0psi @09:40:40.45 SS.reconfigure forSampling @09:40:40.48 SS.seek 25.00ml @09:40:42.16 Vacuum too high -- waiting up to 40s @09:40:42.64 Intake @37.3psia, vacuum from 20.0 to 24.0psi @09:40:42.65 SS.reconfigure forSampling @09:40:42.69 SS.seek 25.00ml @09:40:44.25 Vacuum too high -- waiting up to 40s @09:40:44.94 Intake @37.5psia, vacuum from 20.0 to 24.0psi @09:40:44.95 SS.reconfigure forSampling @09:40:44.99 SS.seek 25.00ml @09:40:46.78 Vacuum too high -- waiting up to 40s @09:40:47.46 Intake @37.8psia, vacuum from 20.0 to 24.0psi @09:40:47.47 SS.reconfigure forSampling @09:40:47.51 SS.seek 25.00ml @09:40:49.22 Vacuum too high -- waiting up to 40s @09:40:49.91 Intake @37.3psia, vacuum from 20.0 to 24.0psi @09:40:49.92 SS.reconfigure forSampling @09:40:49.96 SS.seek 25.00ml @09:40:51.66 Vacuum too high -- waiting up to 40s @09:40:52.37 Intake @37.1psia, vacuum from 20.0 to 24.0psi @09:40:52.39 SS.reconfigure forSampling @09:40:52.42 SS.seek 25.00ml @09:40:54.28 Vacuum too high -- waiting up to 40s @09:40:54.99 Intake @37.4psia, vacuum from 20.0 to 24.0psi @09:40:55.00 SS.reconfigure forSampling @09:40:55.04 SS.seek 25.00ml @09:40:56.72 Vacuum too high -- waiting up to 40s @09:40:57.42 Intake @37.9psia, vacuum from 20.0 to 24.0psi @09:40:58.20 SS.reconfigure forSampling @09:40:58.23 SS.seek 25.00ml @09:41:00.53 Vacuum too high -- waiting up to 40s @09:41:01.24 Intake @37.7psia, vacuum from 20.0 to 24.0psi @09:41:01.26 SS.reconfigure forSampling @09:41:01.29 SS.seek 25.00ml @09:41:02.88 Vacuum too high -- waiting up to 40s @09:41:03.59 Intake @37.6psia, vacuum from 20.0 to 24.0psi @09:41:03.61 SS.reconfigure forSampling @09:41:03.64 SS.seek 25.00ml @09:41:05.22 Vacuum too high -- waiting up to 40s @09:41:05.93 Intake @37.0psia, vacuum from 20.0 to 24.0psi @09:41:05.95 SS.reconfigure forSampling @09:41:05.98 SS.seek 25.00ml @09:41:07.66 Vacuum too high -- waiting up to 40s @09:41:08.37 Intake @37.4psia, vacuum from 20.0 to 24.0psi @09:41:08.39 SS.reconfigure forSampling @09:41:08.42 SS.seek 25.00ml @09:41:10.13 Vacuum too high -- waiting up to 40s @09:41:10.84 Intake @38.0psia, vacuum from 20.0 to 24.0psi @09:41:10.86 SS.reconfigure forSampling @09:41:10.89 SS.seek 25.00ml @09:41:12.38 Vacuum too high -- waiting up to 40s @09:41:13.10 SS.seek 25.00ml @09:41:14.59 Vacuum too high -- waiting up to 40s @09:41:15.30 Intake @37.6psia, vacuum from 20.0 to 24.0psi @09:41:15.32 SS.reconfigure forSampling @09:41:15.35 SS.seek 25.00ml @09:41:17.03 Vacuum too high -- waiting up to 40s @09:41:17.74 SS.seek 25.00ml @09:41:19.25 Vacuum too high -- waiting up to 40s @09:41:19.95 Intake @37.6psia, vacuum from 20.0 to 24.0psi @09:41:19.96 SS.reconfigure forSampling @09:41:20.00 SS.seek 25.00ml @09:41:21.69 Vacuum too high -- waiting up to 40s @09:41:22.40 Intake @37.9psia, vacuum from 20.0 to 24.0psi @09:41:22.42 SS.reconfigure forSampling @09:41:22.45 SS.seek 25.00ml @09:41:23.87 Vacuum too high -- waiting up to 40s @09:41:24.58 Intake @37.7psia, vacuum from 20.0 to 24.0psi @09:41:24.60 SS.reconfigure forSampling @09:41:24.63 SS.seek 25.00ml @09:41:26.28 Vacuum too high -- waiting up to 40s @09:41:26.99 Intake @37.5psia, vacuum from 20.0 to 24.0psi @09:41:27.00 SS.reconfigure forSampling @09:41:27.04 SS.seek 25.00ml @09:41:28.72 Vacuum too high -- waiting up to 40s @09:41:29.42 Intake @38.0psia, vacuum from 20.0 to 24.0psi @09:41:29.44 SS.reconfigure forSampling @09:41:29.47 SS.seek 25.00ml @09:41:30.91 Vacuum too high -- waiting up to 40s @09:41:31.61 Intake @37.7psia, vacuum from 20.0 to 24.0psi @09:41:31.63 SS.reconfigure forSampling @09:41:31.66 SS.seek 25.00ml @09:41:33.22 Vacuum too high -- waiting up to 40s @09:41:33.92 Intake @38.2psia, vacuum from 20.0 to 24.0psi @09:41:33.94 SS.reconfigure forSampling @09:41:33.97 SS.seek 25.00ml @09:41:36.62 Vacuum too high -- waiting up to 40s @09:41:37.33 Intake @37.7psia, vacuum from 20.0 to 24.0psi @09:41:37.35 SS.reconfigure forSampling @09:41:37.38 SS.seek 25.00ml @09:41:38.84 Vacuum too high -- waiting up to 40s @09:41:39.55 Intake @37.4psia, vacuum from 20.0 to 24.0psi @09:41:39.57 SS.reconfigure forSampling @09:41:39.60 SS.seek 25.00ml @09:41:41.19 Vacuum too high -- waiting up to 40s @09:41:41.90 Intake @37.6psia, vacuum from 20.0 to 24.0psi @09:41:41.92 SS.reconfigure forSampling @09:41:41.95 SS.seek 25.00ml @09:41:43.44 Vacuum too high -- waiting up to 40s @09:41:44.15 Intake @38.4psia, vacuum from 20.0 to 24.0psi @09:41:44.17 SS.reconfigure forSampling @09:41:44.20 SS.seek 25.00ml @09:41:45.72 Vacuum too high -- waiting up to 40s @09:41:46.43 Intake @37.9psia, vacuum from 20.0 to 24.0psi @09:41:46.45 SS.reconfigure forSampling @09:41:46.48 SS.seek 25.00ml @09:41:48.06 Vacuum too high -- waiting up to 40s @09:41:48.77 Intake @37.5psia, vacuum from 20.0 to 24.0psi @09:41:48.79 SS.reconfigure forSampling @09:41:48.82 SS.seek 25.00ml @09:41:50.37 Vacuum too high -- waiting up to 40s @09:41:51.08 Intake @37.4psia, vacuum from 20.0 to 24.0psi @09:41:51.10 SS.reconfigure forSampling @09:41:51.13 SS.seek 25.00ml @09:41:52.59 Vacuum too high -- waiting up to 40s @09:41:53.28 Intake @37.3psia, vacuum from 20.0 to 24.0psi @09:41:53.30 SS.reconfigure forSampling @09:41:53.33 SS.seek 25.00ml @09:41:54.78 Vacuum too high -- waiting up to 40s @09:41:55.49 Intake @37.9psia, vacuum from 20.0 to 24.0psi @09:41:55.51 SS.reconfigure forSampling @09:41:55.54 SS.seek 25.00ml @09:41:56.94 Vacuum too high -- waiting up to 40s @09:41:57.64 Intake @38.4psia, vacuum from 20.0 to 24.0psi @09:41:57.66 SS.reconfigure forSampling @09:41:57.69 SS.seek 25.00ml @09:41:59.06 Vacuum too high -- waiting up to 40s @09:41:59.56 Intake @37.5psia, vacuum from 20.0 to 24.0psi @09:41:59.57 SS.reconfigure forSampling @09:41:59.60 SS.seek 25.00ml @09:42:01.00 Vacuum too high -- waiting up to 40s @09:42:01.72 SS.seek 25.00ml @09:42:03.06 Vacuum too high -- waiting up to 40s @09:42:03.76 Intake @37.6psia, vacuum from 20.0 to 24.0psi @09:42:03.78 SS.reconfigure forSampling @09:42:03.81 SS.seek 25.00ml @09:42:05.31 Vacuum too high -- waiting up to 40s @09:42:06.01 Intake @37.5psia, vacuum from 20.0 to 24.0psi @09:42:06.03 SS.reconfigure forSampling @09:42:06.06 SS.seek 25.00ml @09:42:07.43 Vacuum too high -- waiting up to 40s @09:42:08.13 Intake @37.5psia, vacuum from 20.0 to 24.0psi @09:42:08.15 SS.reconfigure forSampling @09:42:08.18 SS.seek 25.00ml @09:42:10.22 Vacuum too high -- waiting up to 40s @09:42:10.49 Intake @38.4psia, vacuum from 20.0 to 24.0psi @09:42:10.51 SS.reconfigure forSampling @09:42:10.54 SS.seek 25.00ml @09:42:12.06 Vacuum too high -- waiting up to 40s @09:42:12.55 Intake @37.4psia, vacuum from 20.0 to 24.0psi @09:42:12.57 SS.reconfigure forSampling @09:42:12.60 SS.seek 25.00ml @09:42:14.00 Vacuum too high -- waiting up to 40s @09:42:14.07 delay 3 seconds @09:42:14.71 Intake @37.6psia, vacuum from 20.0 to 24.0psi @09:42:14.73 SS.reconfigure forSampling @09:42:14.76 SS.seek 25.00ml @09:42:16.31 Vacuum too high -- waiting up to 40s @09:42:17.02 Intake @37.8psia, vacuum from 20.0 to 24.0psi @09:42:17.04 SS.reconfigure forSampling @09:42:17.07 SS.seek 25.00ml @09:42:17.11 ATV.dial :air3 @09:42:17.47 delay 3 seconds @09:42:18.37 Vacuum too high -- waiting up to 40s @09:42:19.08 Intake @37.2psia, vacuum from 20.0 to 24.0psi @09:42:19.10 SS.reconfigure forSampling @09:42:19.13 SS.seek 25.00ml @09:42:20.52 AS.seek 0.604ml @09:42:20.78 Vacuum too high -- waiting up to 40s @09:42:21.49 Intake @37.9psia, vacuum from 20.0 to 24.0psi @09:42:21.51 SS.reconfigure forSampling @09:42:21.54 SS.seek 25.00ml @09:42:22.87 Vacuum too high -- waiting up to 40s @09:42:23.59 Intake @38.1psia, vacuum from 20.0 to 24.0psi @09:42:23.60 SS.reconfigure forSampling @09:42:23.63 SS.seek 25.00ml @09:42:25.03 Vacuum too high -- waiting up to 40s @09:42:25.73 Intake @37.6psia, vacuum from 20.0 to 24.0psi @09:42:25.75 SS.reconfigure forSampling @09:42:25.78 SS.seek 25.00ml @09:42:25.88 delay 3 seconds @09:42:27.18 Vacuum too high -- waiting up to 40s @09:42:27.90 SS.seek 25.00ml @09:42:28.89 ATV.dial :bleach @09:42:29.22 Vacuum too high -- waiting up to 40s @09:42:29.30 AS.seek 0.589ml @09:42:29.93 Intake @37.7psia, vacuum from 20.0 to 24.0psi @09:42:29.95 SS.reconfigure forSampling @09:42:29.98 SS.seek 25.00ml @09:42:31.43 Vacuum too high -- waiting up to 40s @09:42:32.13 Intake @38.1psia, vacuum from 20.0 to 24.0psi @09:42:32.15 SS.reconfigure forSampling @09:42:32.18 SS.seek 25.00ml @09:42:32.61 delay 3 seconds @09:42:33.62 Vacuum too high -- waiting up to 40s @09:42:34.33 SS.seek 25.00ml @09:42:35.59 Vacuum too high -- waiting up to 40s @09:42:35.62 ATV.dial :ASV @09:42:36.30 Intake @37.8psia, vacuum from 20.0 to 24.0psi @09:42:36.32 SS.reconfigure forSampling @09:42:36.35 SS.seek 25.00ml @09:42:36.56 ASV.dial :wasteR @09:42:37.09 AS.seek 0.000ml @09:42:37.81 Vacuum too high -- waiting up to 40s @09:42:38.51 Intake @37.4psia, vacuum from 20.0 to 24.0psi @09:42:38.52 SS.reconfigure forSampling @09:42:38.56 SS.seek 25.00ml @09:42:40.00 Vacuum too high -- waiting up to 40s @09:42:40.92 Intake @37.3psia, vacuum from 20.0 to 24.0psi @09:42:40.94 SS.reconfigure forSampling @09:42:40.97 SS.seek 25.00ml @09:42:42.65 Vacuum too high -- waiting up to 40s @09:42:43.58 Intake @38.2psia, vacuum from 20.0 to 24.0psi @09:42:43.60 SS.reconfigure forSampling @09:42:43.63 SS.seek 25.00ml @09:42:45.18 Vacuum too high -- waiting up to 40s @09:42:45.91 Intake @37.7psia, vacuum from 20.0 to 24.0psi @09:42:45.92 SS.reconfigure forSampling @09:42:45.95 SS.seek 25.00ml @09:42:47.37 Vacuum too high -- waiting up to 40s @09:42:48.08 Intake @37.5psia, vacuum from 20.0 to 24.0psi @09:42:48.10 SS.reconfigure forSampling @09:42:48.13 SS.seek 25.00ml @09:42:49.46 Vacuum too high -- waiting up to 40s @09:42:50.17 Intake @37.7psia, vacuum from 20.0 to 24.0psi @09:42:50.19 SS.reconfigure forSampling @09:42:50.22 SS.seek 25.00ml @09:42:51.56 Vacuum too high -- waiting up to 40s @09:42:51.58 SS.reconfigure SSconfig @09:42:51.61 SS.hold @09:42:51.70 Intake.select :close @09:42:51.85 ShallowSampler::Clogged in WCR -- progress too slow after 224.1ml @09:42:51.86 SSV.dial :exhaust @09:42:52.65 Exhaust.select :open @09:42:52.75 SS.reconfigure SSexhale @09:42:52.79 SS.seek 0.00ml @09:43:01.50 Exhaust.select :close @09:43:01.61 Sampled 224.1ml @09:43:01.62 collectSample: Complete @09:43:01.63 Drying puck before fixing for 04:00.00 @09:43:01.64 CBV.dial :waste2b @09:43:02.43 delay 1:00 @09:43:04.60 Sleepy.power :sampler,:OFF @09:44:02.43 CSV.dial :air @09:44:02.88 CS.reconfigure CSfast @09:44:02.91 CS.seek 10.000ml @09:44:26.98 CBV.dial between CSV and waste2b @09:44:27.30 Pumping wcr puck from tube 2 up to 55psi... @09:44:27.34 CBV.dial SSV @09:44:28.77 CBV.dial between CSV and waste2b @09:44:29.72 CSV.dial between air and CTV @09:44:30.12 CS.seek 7.003ml @09:44:37.51 delay 1:00 @09:44:37.80 CTV.dial CSV,avoiding => air @09:44:37.82 CSV.dial CTV @09:44:38.57 CS.reconfigure CSpressurize @09:44:38.60 CS.seek 0.000ml @09:44:53.41 CBV.dial :waste2b @09:44:53.72 CS.reconfigure CSconfig @09:44:53.75 CS.seek empty @09:44:57.89 delay 1:00 @09:45:37.52 AFV.select :carrierSolution @09:45:37.61 delay 2 seconds @09:45:39.62 AS.reconfigure ASconfig @09:45:39.65 AS.seek 1.000ml @09:45:57.90 CTV.dial :air,avoiding => intake @09:45:59.18 Fix #1 of 2 (with rnal) for 02:00.00 @09:45:59.19 CS Pulling 2ml of rnal CSV.dial CSR @09:45:59.62 CSR.select :rnal @09:45:59.72 CS.seek 2.000ml @09:46:05.66 delay 5 seconds @09:46:10.67 CSR.select :air @09:46:10.76 CS.reconfigure CSfast @09:46:10.80 CS.seek 10.000ml @09:46:30.10 CBV.dial between CSV and waste2b @09:46:30.42 Pumping wcr puck from tube 2 up to 55psi... @09:46:30.46 CBV.dial SSV @09:46:31.89 CBV.dial between CSV and waste2b @09:46:32.84 CSV.dial between air and CTV @09:46:33.50 CS.seek 6.966ml @09:46:38.23 delay 1 second @09:46:39.24 AFV.select :holdingCoil @09:46:39.33 delay 1 second @09:46:40.34 ATV.dial :ASV @09:46:40.35 ASV.dial :wasteR @09:46:40.36 AS.reconfigure ASslow1 @09:46:40.39 AS.seek 0.000ml @09:46:41.30 CTV.dial CSV,avoiding => air @09:46:41.32 CSV.dial CTV @09:46:42.67 CS.reconfigure CSpressurize @09:46:42.70 CS.seek 0.000ml @09:46:59.75 delay 2:00 @09:48:59.77 CBV.dial :waste2b @09:49:00.10 CS.reconfigure CSconfig @09:49:00.13 CS.seek empty @09:49:01.95 delay 1:00 @09:50:01.96 CTV.dial :air,avoiding => intake @09:50:03.25 Fix #2 of 2 (with rnal) for 02:00.00 @09:50:03.26 CS Pulling 2ml of rnal CSV.dial CSR @09:50:04.16 CSR.select :rnal @09:50:04.26 CS.seek 2.000ml @09:50:04.93 delay 20 seconds @09:50:10.13 delay 5 seconds @09:50:15.14 CSR.select :air @09:50:15.24 CS.reconfigure CSfast @09:50:15.28 CS.seek 10.000ml @09:50:24.94 ATV.dial :air3 @09:50:26.06 AS.seek 0.590ml @09:50:34.57 CBV.dial between CSV and waste2b @09:50:34.91 Pumping wcr puck from tube 2 up to 55psi... @09:50:34.96 CBV.dial SSV @09:50:36.39 CBV.dial between CSV and waste2b @09:50:37.34 CSV.dial between air and CTV @09:50:38.00 CS.seek 6.855ml @09:50:45.98 CTV.dial CSV,avoiding => air @09:50:46.00 CSV.dial CTV @09:50:47.30 CS.reconfigure CSpressurize @09:50:47.33 CS.seek 0.000ml @09:51:04.06 delay 2:00 @09:52:26.62 delay 3 seconds @09:52:29.63 ATV.dial :ASV @09:52:30.71 ASV.dial :wasteR @09:52:30.77 AS.seek 0.090ml @09:53:04.07 CBV.dial :waste2b @09:53:04.40 CS.reconfigure CSconfig @09:53:04.43 CS.seek empty @09:53:06.22 delay 1:00 @09:54:06.23 CTV.dial :air,avoiding => intake @09:54:07.55 Storing wcr puck in Collection Clamp to tube 1 @09:54:07.69 SC.reconfigure SCtight @09:54:07.72 SC.seek 1 @09:54:09.97 SC.reconfigure SCloose @09:54:10.15 Elbow.reconfigure TightElbow @09:54:10.18 Elbow.seek 1 @09:54:11.82 Forearm.reconfigure TightForearm @09:54:11.85 Forearm.seek 1 + 800 counts @09:54:12.94 SE.reconfigure SEpick @09:54:13.00 delay 30 seconds @09:54:13.02 SE.seek beamBreak @09:54:14.71 SE.configure SEconfig @09:54:14.83 Elbow.reconfigure LimpElbow @09:54:14.87 Forearm.reconfigure LooseForearm @09:54:14.91 Forearm.seek 1 @09:54:15.12 Elbow.hold @09:54:15.17 Elbow.reconfigure TightElbow @09:54:15.25 SE.seek 13.05 Pucks below Top Plate @09:54:15.59 Forearm.coast @09:54:15.63 Forearm.seek 1 + 326 counts @09:54:15.86 Forearm.reconfigure TightForearm @09:54:15.90 Forearm.seek 1 + 800 counts @09:54:16.18 SE.seek 14.05 Pucks below Top Plate @09:54:16.79 Forearm.reconfigure LooseForearm @09:54:16.82 Forearm.seek retract @09:54:17.77 Elbow.seek CC @09:54:19.32 Elbow.reconfigure LooseElbow @09:54:19.41 Forearm.seek CC @09:54:20.35 CC.seek sealed @09:54:21.25 Hand.grip @09:54:22.02 CC.seek open @09:54:25.39 Forearm.seek retract @09:54:26.37 Elbow.reconfigure TightElbow @09:54:27.01 Elbow.seek 1 @09:54:28.60 Elbow.reconfigure LimpElbow @09:54:28.65 Forearm.seek 1 @09:54:29.66 Elbow.hold @09:54:29.71 Elbow.reconfigure TightElbow @09:54:29.74 Hand.release @09:54:30.60 Forearm.coast @09:54:30.64 Forearm.seek 1 + 331 counts @09:54:30.92 Forearm.reconfigure TightForearm @09:54:30.96 Forearm.seek 1 + 800 counts @09:54:31.27 SE.seek down @09:54:32.37 Forearm.reconfigure LooseForearm @09:54:32.40 Forearm.seek retract @09:54:32.58 SE.coast @09:54:33.36 SC.coast @09:54:33.39 Starting finalPurge @09:54:33.40 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @09:54:33.56 Elbow.seek FlushPuck::Garage @09:54:35.34 Elbow.reconfigure LooseElbow @09:54:35.42 Forearm.seek garage @09:54:36.45 Hand.grip @09:54:37.24 Forearm.seek retract @09:54:38.26 Elbow.reconfigure TightElbow @09:54:38.29 Elbow.seek CC @09:54:41.14 Elbow.reconfigure LooseElbow @09:54:41.22 Forearm.reconfigure TightForearm @09:54:41.25 Forearm.seek CC @09:54:42.33 CC.seek closed @09:54:43.02 ATV.dial :extSrc @09:54:43.65 delay 3 seconds @09:54:46.11
Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 2.03 @09:54:46.16 Hand.release @09:54:46.66 ATV.dial :ElutionWater @09:54:46.94
Forearm.reconfigure LooseForearm @09:54:46.97 Forearm.seek retract @09:54:47.60 AS.seek 0.120ml @09:54:47.96
Flushing the Collection Valve::Manifold @09:54:48.06 CS Pulling 5ml of flush @09:54:48.07 CSV.dial CSR @09:54:48.49 CSR.select :flush @09:54:48.59 CS.seek 5.000ml @09:54:54.02 ATV.dial :air3 @09:54:54.40 AS.seek 0.130ml @09:54:56.69 delay 2 seconds @09:54:58.70 ATV.dial :bleach @09:54:59.07 AS.seek 0.230ml @09:55:02.12
delay 5 seconds @09:55:07.13 CSR.select :air @09:55:07.27 CS.reconfigure CSfast @09:55:07.30 CS.seek 7.500ml @09:55:13.93 CSV.dial :waste @09:55:15.21 CS.reconfigure CSconfig @09:55:15.24 CS.seek 0.000ml @09:55:19.71 delay 3 seconds @09:55:22.72 ATV.dial :air3 @09:55:23.06 delay 3 seconds @09:55:26.12 AS.seek 0.255ml @09:55:31.47 delay 3 seconds @09:55:34.48 ATV.dial :bleach @09:55:34.86 AS.seek 0.240ml @09:55:35.24
Flushing lower manifold's waste1 port @09:55:35.33 CSV.dial :flush @09:55:35.77 CS.reconfigure CSfast @09:55:35.80 CS.seek 5.000ml @09:55:38.16 delay 3 seconds @09:55:41.17 ATV.dial :ASV @09:55:42.78 ASV.dial :SPEdelivery @09:55:43.53 AFV.select :carrierSolution @09:55:43.63 delay 2 seconds @09:55:45.64 AS.reconfigure ASconfig @09:55:45.67 AS.seek 0.500ml @09:55:48.05
delay 5 seconds @09:55:53.06 CSV.dial :air @09:55:53.53 CS.seek 7.000ml @09:55:58.97 CSV.dial CTV @09:55:58.99
CTV.dial CSV @09:56:00.32 CBV.dial :waste1 @09:56:01.06 CS.seek 0.000ml @09:56:01.11 delay 1 second @09:56:02.12 AFV.select :holdingCoil @09:56:02.26 AS.reconfigure ASslow1 @09:56:02.29 AS.seek 0.170ml @09:56:17.77
Flushing lower manifold's waste2b port @09:56:17.87 CSV.dial :flush @09:56:18.67 CS.seek 5.000ml @09:56:30.93 delay 5 seconds @09:56:35.94 CSV.dial :air @09:56:36.51 CS.seek 7.000ml @09:56:41.90 CSV.dial CTV @09:56:41.92
CTV.dial CSV @09:56:42.36 CBV.dial :waste2b @09:56:43.09 CS.seek 0.000ml @09:56:59.64 Flushing upper manifold @09:56:59.73 CSV.dial :flush @09:57:00.73 CS.seek 5.000ml @09:57:09.85 SPE.hold 2:46:40,95.0C @09:57:09.87 delay 3:00 @09:57:12.93
delay 5 seconds @09:57:17.94 CSV.dial :air @09:57:18.43 CS.seek 6.999ml @09:57:24.02 CSV.dial CBV @09:57:24.04
CBV.dial CSV @09:57:25.38 CTV.dial :air @09:57:26.70 CS.seek 0.000ml @09:57:43.95 delay 7 seconds @09:57:50.96 CS.seek 3.000ml @09:57:59.02 CSV.dial :waste @09:57:59.46 CS.seek 0.000ml @09:58:07.42 CSV.dial :flush @09:58:07.87 CS.seek 3.000ml @09:58:15.77 delay 5 seconds @09:58:20.78 CSV.dial :air @09:58:21.27 CS.seek 5.000ml @09:58:26.83 CSV.dial :air @09:58:26.85 CS.seek 0.000ml @09:58:39.42 delay 7 seconds @09:58:46.43 CS.seek 3.000ml @09:58:54.39 CSV.dial :waste @09:58:55.21 CS.seek 0.000ml @09:59:03.14 Disinfecting external Intake with mfbkill @09:59:03.23 CSV.dial CSR @09:59:04.53 CSR.select :mfbkill @09:59:04.63 CS.seek 8.000ml @09:59:24.51 delay 5 seconds @09:59:29.52 CSR.select :air @09:59:29.61 CS.seek 10.000ml @09:59:35.20 CSV.dial CBV @09:59:35.22
CBV.dial CSV @09:59:36.71 CTV.dial :intake @09:59:37.45 Intake.select :open @09:59:37.55 CS.seek 2.000ml @09:59:57.51 Intake.select :close @09:59:57.60 CTV.dial :air @09:59:58.38 CS.reconfigure CSconfig @09:59:58.41 CS.seek 0.000ml @10:00:04.32 Flushing lower manifold's waste1 port @10:00:04.33 CS Pulling 4ml of mfbkill @10:00:04.34 CSV.dial CSR @10:00:05.42 CSR.select :mfbkill @10:00:05.52 CS.seek 4.000ml @10:00:09.88 AFV.select :carrierSolution @10:00:09.97 delay 2 seconds @10:00:11.98 AS.reconfigure ASconfig @10:00:12.02 AS.seek 1.000ml @10:00:16.51
delay 5 seconds @10:00:21.52 CSR.select :air @10:00:21.61 CS.reconfigure CSfast @10:00:21.65 CS.seek 10.000ml @10:00:36.55 CSV.dial CTV @10:00:36.57
CTV.dial CSV @10:00:37.93 CBV.dial :waste1 @10:00:38.39 CS.seek 0.000ml @10:01:00.66 delay 1 second @10:01:01.67 AFV.select :holdingCoil @10:01:01.76 delay 1 second @10:01:02.26
Flushing lower manifold's waste2b port @10:01:02.27 CS Pulling 4ml of mfbkill @10:01:02.28 CSV.dial CSR @10:01:02.77 ASV.dial :wasteD @10:01:02.80
CSR.select :mfbkill @10:01:02.92 CS.reconfigure CSconfig @10:01:02.96 CS.seek 4.000ml @10:01:03.69 AS.seek 0.000ml @10:01:14.45
delay 5 seconds @10:01:19.46 CSR.select :air @10:01:19.56 CS.reconfigure CSfast @10:01:19.60 CS.seek 10.000ml @10:01:34.39 CSV.dial CTV @10:01:34.41
CTV.dial CSV @10:01:34.94 CBV.dial :waste2b @10:01:35.70 CS.seek 0.000ml @10:01:59.32 Killing upper manifold @10:01:59.33 CS Pulling 4ml of mfbkill @10:01:59.34 CSV.dial CSR @10:01:59.85 CSR.select :mfbkill @10:01:59.95 CS.reconfigure CSconfig @10:01:59.99 CS.seek 4.000ml @10:02:02.27 Priming Eluate @10:02:02.28 AFV.select :carrierSolution @10:02:02.37 delay 2 seconds @10:02:04.38 AS.seek 0.500ml @10:02:11.13
delay 5 seconds @10:02:16.14 CSR.select :air @10:02:16.23 CS.reconfigure CSfast @10:02:16.27 CS.seek 10.000ml @10:02:30.91 CSV.dial CBV @10:02:30.93
CBV.dial CSV @10:02:31.96 CTV.dial :air @10:02:33.26 CS.seek 0.000ml @10:02:33.80 delay 1 second @10:02:34.81 AFV.select :holdingCoil @10:02:34.90 ATV.dial ASV @10:02:34.91 ASV.dial :SPErecovery @10:02:35.57 Swish #1 of 2 @10:02:35.62 AS.reconfigure ASslow0 @10:02:35.66 AS.seek 0.600ml @10:02:46.19 AS.seek 0.500ml @10:02:56.71 Swish #2 of 2 @10:02:56.77 AS.seek 0.600ml @10:02:56.91
delay 7 seconds @10:03:03.92 CS.seek 3.000ml @10:03:07.29 AS.seek 0.500ml @10:03:11.72
CSV.dial :waste @10:03:12.17 CS.seek 0.000ml @10:03:17.80 delay 3 seconds @10:03:19.80
Flushing upper manifold @10:03:19.90 CSV.dial :flush @10:03:20.35 CS.seek 5.000ml @10:03:20.86 AS.reconfigure ASslow1 @10:03:20.89 AS.seek 0.676ml @10:03:32.72
delay 5 seconds @10:03:37.73 CSV.dial :air @10:03:38.25 CS.seek 7.001ml @10:03:43.78 CSV.dial CBV @10:03:43.80
CBV.dial CSV @10:03:44.90 CTV.dial :air @10:03:44.91 CS.seek 0.000ml @10:03:57.02 delay 3 seconds @10:04:00.03 Eluate primed @10:04:00.04 ATV.dial :waste2 @10:04:00.67 AS.reconfigure ASnorm @10:04:00.70 AS.seek 0.000ml @10:04:01.59
delay 7 seconds @10:04:08.60 CS.seek 3.000ml @10:04:16.31 CSV.dial :waste @10:04:16.82 CS.seek 0.000ml @10:04:24.62 CSV.dial :flush @10:04:25.27 CS.seek 3.000ml @10:04:32.97 delay 5 seconds @10:04:35.58 SPE.coast @10:04:35.66 ASV.dial :SPErecovery @10:04:35.67 Setting up PCR for HF183em -- writing /var/log/esp/HF183em14mar0810h195ml.pcr @10:04:35.69 Building PCR Slug from ASV with MM2 mix, PP4 primer Priming with carrier @10:04:35.70 ATV.dial ARV @10:04:36.39 ARV.dial :carrier @10:04:36.69 AS.seek 0.500ml @10:04:37.98
CSV.dial :air @10:04:38.55 CS.seek 4.999ml @10:04:43.97 CSV.dial :air @10:04:43.99 CS.seek 0.000ml @10:04:56.06 delay 7 seconds @10:05:02.56 AFV.select :carrierSolution @10:05:02.67 delay 2 seconds @10:05:03.08
CS.seek 3.000ml @10:05:04.69 AS.reconfigure ASconfig @10:05:04.73 AS.seek 1.000ml @10:05:10.69
CSV.dial :waste @10:05:11.49 CS.seek 0.000ml @10:05:19.04 Flushing lower manifold's waste1 port @10:05:19.13 CSV.dial :flush @10:05:19.64 CS.seek 5.000ml @10:05:31.82 delay 5 seconds @10:05:34.18 delay 1 second @10:05:35.19 AFV.select :holdingCoil @10:05:35.29 delay 1 second @10:05:36.30 ATV.dial :waste @10:05:36.83
CSV.dial :air @10:05:37.31 CS.seek 7.000ml @10:05:37.56 AS.reconfigure ASnorm @10:05:37.59 AS.seek empty @10:05:42.69 CSV.dial CTV @10:05:42.71
CTV.dial CSV @10:05:44.64 CBV.dial :waste1 @10:05:45.09 CS.seek 0.000ml @10:06:01.50 Flushing lower manifold's waste2b port @10:06:01.59 CSV.dial :flush @10:06:02.39 CS.seek 5.000ml @10:06:14.50 delay 5 seconds @10:06:19.51 CSV.dial :air @10:06:20.05 CS.seek 6.999ml @10:06:25.42 CSV.dial CTV @10:06:25.43
CTV.dial CSV @10:06:25.86 CBV.dial :waste2b @10:06:26.61 CS.seek 0.000ml @10:06:29.08 AFV.select :carrierSolution @10:06:29.19 delay 2 seconds @10:06:31.20 AS.reconfigure ASconfig @10:06:31.24 AS.seek 0.500ml @10:06:43.03
Disinfecting external Intake with kill @10:06:43.12 CSV.dial CSR @10:06:43.55 CSR.select :kill @10:06:43.65 CS.seek 8.000ml @10:07:00.74 delay 1 second @10:07:01.75 AFV.select :holdingCoil @10:07:01.85 Building Slug @10:07:01.86 ATV.dial :ARV @10:07:02.60
delay 5 seconds @10:07:03.12 delay 2 seconds @10:07:05.12 ARV.dial :air1 @10:07:05.47 delay 2 seconds @10:07:07.52 AS.reconfigure ASslow2 @10:07:07.55 AS.seek 0.506ml @10:07:07.61
CSR.select :air @10:07:07.70 CS.seek 10.000ml @10:07:13.13 CSV.dial CBV @10:07:13.15
CBV.dial CSV @10:07:13.85 delay 2 seconds @10:07:14.18
CTV.dial :intake @10:07:14.89 Intake.select :open @10:07:14.99 CS.seek 2.000ml @10:07:15.86 ARV.dial :MM2 @10:07:17.09 delay 2 seconds @10:07:19.09 Adding MM2 @10:07:19.15 AS.seek 0.524ml @10:07:34.19
Intake.select :close @10:07:34.28 CTV.dial :air @10:07:34.99 CS.reconfigure CSconfig @10:07:35.02 CS.seek 0.000ml @10:07:37.69 delay 25 seconds @10:07:40.91
CSV.dial CSR @10:07:41.95 CSR.select :flush @10:07:42.05 CS.seek 5.000ml @10:07:55.50 delay 5 seconds @10:08:00.51 CSR.select :air @10:08:00.60 CS.seek 10.000ml @10:08:02.70 ARV.dial :PP4 @10:08:03.04 Adding PP4 @10:08:03.05 delay 2 seconds @10:08:05.10 AS.seek 0.530ml @10:08:11.35 delay 10 seconds @10:08:14.12
CSV.dial CBV @10:08:15.18 CS.seek 0.000ml @10:08:21.36 ARV.dial :air3 @10:08:21.67 adding ARV air @10:08:21.68 delay 2 seconds @10:08:23.74 AS.seek 0.536ml @10:08:30.24 delay 5 seconds @10:08:35.25 ARV.dial :carrier @10:08:36.28 delay 7 seconds @10:08:41.40
Garaging FlushPuck in Collection Clamp @10:08:41.46 Elbow.reconfigure TightElbow @10:08:41.49 Elbow.seek CC @10:08:41.77 Elbow.reconfigure LooseElbow @10:08:41.85 Forearm.seek CC @10:08:42.84 CC.seek sealed @10:08:43.34 AS.seek 0.594ml @10:08:43.78
Hand.grip @10:08:44.49 CC.seek open @10:08:47.80 Forearm.seek retract @10:08:48.76 Elbow.reconfigure TightElbow @10:08:48.80 Elbow.seek garage @10:08:51.58 Elbow.reconfigure LooseElbow @10:08:51.66 Forearm.reconfigure TightForearm @10:08:51.69 Forearm.seek garage @10:08:52.84 Hand.release @10:08:53.66 Forearm.reconfigure LooseForearm @10:08:53.69 Forearm.seek retract @10:09:42.69 delay 5 seconds @10:09:47.70 ATV.dial :ASV @10:09:48.03 delay 2 seconds @10:09:50.09 AS.seek 0.600ml @10:09:57.12 delay 4 seconds @10:10:01.13 ATV.dial ARV @10:10:01.56 delay 2 seconds @10:10:03.57 ARV.dial :carrier @10:10:03.58 delay 2 seconds @10:10:05.63 AS.seek 0.650ml @10:10:56.86 delay 4 seconds @10:11:00.87 ATV.dial PCR @10:11:01.39 ASV.dial :waste1 @10:11:02.29 ARV.dial :air1 @10:11:02.63 Positioning Slug by 0.324ml @10:11:02.68 AS.seek 0.326ml @10:16:33.19 delay 5 seconds @10:16:38.20 ATV.dial ARV @10:16:38.72 ASV.dial :PCRlock @10:16:39.31 delay 5 seconds @10:16:44.32 Running HF183em ProfileEM60C2 thermocycling @10:16:44.33 PCR.program ProfileEM60C2 @10:16:44.44 ProfileEM60C2 should complete in < 2:11:38 @10:16:44.45 PCR.run 2:49:32.5 @12:12:49.56 PCR.off @12:12:50.08 PCR for HF183em completed -- closed /var/log/esp/HF183em14mar0810h195ml.pcr @12:12:50.13 Cleaning PCR @12:12:50.14 AFV.select :carrierSolution @12:12:50.23 delay 2 seconds @12:12:52.24 AS.reconfigure ASconfig @12:12:52.27 AS.seek 0.500ml @12:13:02.69 delay 1 second @12:13:03.70 AFV.select :holdingCoil @12:13:03.79 ASV.dial :waste1 @12:13:04.47 ATV.dial :air3 @12:13:05.41 AS.reconfigure ASslow1 @12:13:05.44 AS.seek 0.510ml @12:13:07.66 delay 2 seconds @12:13:09.67 ATV.dial :bleach @12:13:10.16 AS.seek 0.810ml @12:14:11.58 delay 3 seconds @12:14:14.59 ATV.dial :air3 @12:14:14.92 delay 3 seconds @12:14:17.98 AS.seek 0.835ml @12:14:23.31 delay 3 seconds @12:14:26.32 ATV.dial :bleach @12:14:26.71 AS.seek 0.820ml @12:14:30.03 delay 3 seconds @12:14:33.04 ARV.dial :waste @12:14:33.53 delay 3 seconds @12:14:36.54 ATV.dial :ARV @12:14:37.26 AS.reconfigure ASconfig @12:14:37.30 AS.seek 0.470ml @12:14:57.97 AFV.select :carrierSolution @12:14:58.06 delay 2 seconds @12:15:00.66 AS.seek 1.000ml @12:15:31.86 delay 1 second @12:15:32.87 AFV.select :holdingCoil @12:15:32.96 delay 1 second @12:15:33.97 AS.reconfigure ASfast @12:15:34.00 AS.seek 0.000ml @12:16:01.75 AFV.select :carrierSolution @12:16:01.85 delay 2 seconds @12:16:03.86 AS.reconfigure ASconfig @12:16:03.89 AS.seek 1.000ml @12:17:02.50 delay 1 second @12:17:03.51 AFV.select :holdingCoil @12:17:03.60 delay 1 second @12:17:04.61 AS.reconfigure ASfast @12:17:04.65 AS.seek 0.000ml @12:17:32.37 AFV.select :carrierSolution @12:17:32.47 delay 2 seconds @12:17:34.48 AS.reconfigure ASconfig @12:17:34.51 AS.seek 1.000ml @12:18:33.10 delay 1 second @12:18:34.11 AFV.select :holdingCoil @12:18:34.20 delay 1 second @12:18:35.21 AS.reconfigure ASfast @12:18:35.25 AS.seek 0.000ml @12:19:02.99 ARV.dial between carrier and air1 @12:19:03.40 ATV.dial :air3 @12:19:04.43 AS.reconfigure ASslow1 @12:19:04.46 AS.seek 0.010ml @12:19:06.79 delay 2 seconds @12:19:08.80 ATV.dial :bleach @12:19:09.29 AS.seek 0.810ml @12:21:52.71 delay 3 seconds @12:21:55.72 ATV.dial :air3 @12:21:56.04 delay 3 seconds @12:21:59.10 AS.seek 0.835ml @12:22:04.43 delay 3 seconds @12:22:07.44 ATV.dial :bleach @12:22:07.83 AS.seek 0.820ml @12:22:11.12 delay 3 seconds @12:22:14.13 ATV.dial PCR @12:22:14.46 AS.reconfigure ASconfig @12:22:14.49 AS.seek empty @12:23:02.63 PCR.program :bleachProfile @12:23:02.68 PCR program should complete in < 2:45 @12:23:02.69 PCR.run 10:00 @12:26:00.88 PCR.off @12:26:00.91 AFV.select :carrierSolution @12:26:01.00 delay 2 seconds @12:26:03.01 AS.seek 1.000ml @12:27:01.67 delay 1 second @12:27:02.68 AFV.select :holdingCoil @12:27:02.77 delay 1 second @12:27:03.78 AS.reconfigure ASfast @12:27:03.82 AS.seek 0.000ml @12:27:31.56 delay 3 seconds @12:27:34.57 AFV.select :carrierSolution @12:27:34.66 delay 2 seconds @12:27:36.67 AS.reconfigure ASconfig @12:27:36.70 AS.seek 1.000ml @12:28:35.31 delay 1 second @12:28:36.32 AFV.select :holdingCoil @12:28:36.41 delay 1 second @12:28:37.42 AS.reconfigure ASfast @12:28:37.45 AS.seek 0.000ml @12:29:05.20 delay 3 seconds @12:29:08.21 AFV.select :carrierSolution @12:29:08.30 delay 2 seconds @12:29:10.31 AS.reconfigure ASconfig @12:29:10.34 AS.seek 1.000ml @12:30:08.95 delay 1 second @12:30:09.96 AFV.select :holdingCoil @12:30:10.10 delay 1 second @12:30:11.11 AS.reconfigure ASfast @12:30:11.15 AS.seek 0.000ml @12:30:38.90 delay 3 seconds @12:30:41.91 AFV.select :carrierSolution @12:30:42.00 delay 2 seconds @12:30:44.01 AS.reconfigure ASconfig @12:30:44.04 AS.seek 1.000ml @12:31:42.62 delay 1 second @12:31:43.63 AFV.select :holdingCoil @12:31:43.72 delay 1 second @12:31:44.73 AS.reconfigure ASfast @12:31:44.76 AS.seek 0.000ml @12:32:12.46 delay 3 seconds @12:32:15.47 AFV.select :carrierSolution @12:32:15.56 delay 2 seconds @12:32:17.57 AS.reconfigure ASconfig @12:32:17.60 AS.seek 1.000ml @12:33:16.21 delay 1 second @12:33:17.22 AFV.select :holdingCoil @12:33:17.31 delay 1 second @12:33:18.32 AS.reconfigure ASnorm @12:33:18.35 AS.seek 0.000ml @12:34:09.83 Setting up PCR for ENTap -- writing /var/log/esp/ENTap14mar0812h195ml.pcr @12:34:09.84 Building PCR Slug from ASV with MM1 mix, PP1 primer @12:34:09.85 Priming with carrier @12:34:09.86 ATV.dial ARV @12:34:10.38 ARV.dial :carrier @12:34:10.66 AS.seek 0.500ml @12:34:36.58 AFV.select :carrierSolution @12:34:36.67 delay 2 seconds @12:34:38.68 AS.reconfigure ASconfig @12:34:38.71 AS.seek 1.000ml @12:35:08.16 delay 1 second @12:35:09.17 AFV.select :holdingCoil @12:35:09.26 delay 1 second @12:35:10.27 ATV.dial :waste @12:35:11.53 AS.reconfigure ASnorm @12:35:11.56 AS.seek empty @12:36:03.01 AFV.select :carrierSolution @12:36:03.11 delay 2 seconds @12:36:05.12 AS.reconfigure ASconfig @12:36:05.15 AS.seek 0.500ml @12:36:34.64 delay 1 second @12:36:35.65 AFV.select :holdingCoil @12:36:35.74 Building Slug @12:36:35.75 ATV.dial :ARV @12:36:37.00 delay 2 seconds @12:36:39.01 ARV.dial :air1 @12:36:39.34 delay 2 seconds @12:36:41.40 AS.reconfigure ASslow2 @12:36:41.43 AS.seek 0.506ml @12:36:47.72 delay 2 seconds @12:36:49.73 ARV.dial :MM1 @12:36:50.23 delay 2 seconds @12:36:52.24 Adding MM1 @12:36:52.29 AS.seek 0.524ml @12:37:10.87 delay 25 seconds @12:37:35.88 ARV.dial :PP1 @12:37:36.76 Adding PP1 @12:37:36.77 delay 2 seconds @12:37:38.83 AS.seek 0.530ml @12:37:45.43 delay 10 seconds @12:37:55.44 ARV.dial :air1 @12:37:55.75 adding ARV air @12:37:55.76 delay 2 seconds @12:37:57.81 AS.seek 0.536ml @12:38:04.17 delay 5 seconds @12:38:09.18 ARV.dial :carrier @12:38:09.49 delay 7 seconds @12:38:16.55 AS.seek 0.594ml @12:39:15.92 delay 5 seconds @12:39:20.93 ATV.dial :ASV @12:39:21.25 delay 2 seconds @12:39:23.31 AS.seek 0.600ml @12:39:29.77 delay 4 seconds @12:39:33.78 ATV.dial ARV @12:39:34.11 delay 2 seconds @12:39:36.12 ARV.dial :carrier @12:39:36.13 delay 2 seconds @12:39:38.18 AS.seek 0.650ml @12:40:29.37 delay 4 seconds @12:40:33.38 ATV.dial PCR @12:40:33.87 ASV.dial :waste1 @12:40:33.88 ARV.dial :air1 @12:40:34.23 Positioning Slug by 0.324ml @12:40:34.28 AS.seek 0.326ml @12:46:05.16 delay 5 seconds @12:46:10.17 ATV.dial ARV @12:46:10.69 ASV.dial :PCRlock @12:46:11.14 delay 5 seconds @12:46:16.15 Running ENTap Profile60C thermocycling @12:46:16.16 PCR.program Profile60C @12:46:16.26 Profile60C should complete in < 1:39:08 @12:46:16.27 PCR.run 2:08:55 @14:09:59.03 PCR.off @14:09:59.54 PCR for ENTap completed -- closed /var/log/esp/ENTap14mar0812h195ml.pcr @14:09:59.59 Cleaning PCR @14:09:59.60 AFV.select :carrierSolution @14:09:59.70 delay 2 seconds @14:10:01.71 AS.reconfigure ASconfig @14:10:01.74 AS.seek 0.500ml @14:10:12.16 delay 1 second @14:10:13.17 AFV.select :holdingCoil @14:10:13.26 ASV.dial :waste1 @14:10:13.69 ATV.dial :air3 @14:10:14.63 AS.reconfigure ASslow1 @14:10:14.66 AS.seek 0.510ml @14:10:17.55 delay 2 seconds @14:10:19.56 ATV.dial :bleach @14:10:19.99 AS.seek 0.810ml @14:11:21.38 delay 3 seconds @14:11:24.39 ATV.dial :air3 @14:11:24.81 delay 3 seconds @14:11:27.87 AS.seek 0.835ml @14:11:33.24 delay 3 seconds @14:11:36.25 ATV.dial :bleach @14:11:36.74 AS.seek 0.820ml @14:11:40.06 delay 3 seconds @14:11:43.07 ARV.dial :waste @14:11:43.56 delay 3 seconds @14:11:46.57 ATV.dial :ARV @14:11:47.31 AS.reconfigure ASconfig @14:11:47.35 AS.seek 0.470ml @14:12:08.03 AFV.select :carrierSolution @14:12:08.13 delay 2 seconds @14:12:10.14 AS.seek 1.000ml @14:12:41.33 delay 1 second @14:12:42.34 AFV.select :holdingCoil @14:12:42.43 delay 1 second @14:12:43.44 AS.reconfigure ASfast @14:12:43.48 AS.seek 0.000ml @14:13:11.22 AFV.select :carrierSolution @14:13:11.32 delay 2 seconds @14:13:13.33 AS.reconfigure ASconfig @14:13:13.36 AS.seek 1.000ml @14:14:11.98 delay 1 second @14:14:12.99 AFV.select :holdingCoil @14:14:13.08 delay 1 second @14:14:14.09 AS.reconfigure ASfast @14:14:14.12 AS.seek 0.000ml @14:14:41.84 AFV.select :carrierSolution @14:14:41.94 delay 2 seconds @14:14:43.95 AS.reconfigure ASconfig @14:14:43.98 AS.seek 1.000ml @14:15:42.54 delay 1 second @14:15:43.55 AFV.select :holdingCoil @14:15:43.64 delay 1 second @14:15:44.65 AS.reconfigure ASfast @14:15:44.68 AS.seek 0.000ml @14:16:12.41 ARV.dial between carrier and air1 @14:16:12.82 ATV.dial :air3 @14:16:13.75 AS.reconfigure ASslow1 @14:16:13.78 AS.seek 0.010ml @14:16:16.13 delay 2 seconds @14:16:18.14 ATV.dial :bleach @14:16:18.51 AS.seek 0.810ml @14:19:01.92 delay 3 seconds @14:19:04.93 ATV.dial :air3 @14:19:05.41 delay 3 seconds @14:19:08.47 AS.seek 0.835ml @14:19:13.81 delay 3 seconds @14:19:16.82 ATV.dial :bleach @14:19:17.21 AS.seek 0.820ml @14:19:20.52 delay 3 seconds @14:19:23.53 ATV.dial PCR @14:19:23.85 AS.reconfigure ASconfig @14:19:23.88 AS.seek empty @14:20:12.00 PCR.program :bleachProfile @14:20:12.06 PCR program should complete in < 2:45 @14:20:12.07 PCR.run 10:00 @14:23:10.28 PCR.off @14:23:10.31 AFV.select :carrierSolution @14:23:10.40 delay 2 seconds @14:23:12.41 AS.seek 1.000ml @14:24:11.06 delay 1 second @14:24:12.07 AFV.select :holdingCoil @14:24:12.16 delay 1 second @14:24:13.17 AS.reconfigure ASfast @14:24:13.20 AS.seek 0.000ml @14:24:40.95 delay 3 seconds @14:24:43.96 AFV.select :carrierSolution @14:24:44.06 delay 2 seconds @14:24:46.07 AS.reconfigure ASconfig @14:24:46.10 AS.seek 1.000ml @14:25:44.69 delay 1 second @14:25:45.70 AFV.select :holdingCoil @14:25:45.79 delay 1 second @14:25:46.80 AS.reconfigure ASfast @14:25:46.83 AS.seek 0.000ml @14:26:14.51 delay 3 seconds @14:26:17.52 AFV.select :carrierSolution @14:26:17.61 delay 2 seconds @14:26:19.63 AS.reconfigure ASconfig @14:26:19.66 AS.seek 1.000ml @14:27:18.24 delay 1 second @14:27:19.25 AFV.select :holdingCoil @14:27:19.34 delay 1 second @14:27:20.35 AS.reconfigure ASfast @14:27:20.38 AS.seek 0.000ml @14:27:48.12 delay 3 seconds @14:27:51.13 AFV.select :carrierSolution @14:27:51.22 delay 2 seconds @14:27:53.23 AS.reconfigure ASconfig @14:27:53.26 AS.seek 1.000ml @14:28:51.87 delay 1 second @14:28:52.88 AFV.select :holdingCoil @14:28:52.97 delay 1 second @14:28:53.98 AS.reconfigure ASfast @14:28:54.01 AS.seek 0.000ml @14:29:21.74 delay 3 seconds @14:29:24.75 AFV.select :carrierSolution @14:29:24.84 delay 2 seconds @14:29:26.85 AS.reconfigure ASconfig @14:29:26.88 AS.seek 1.000ml @14:30:25.49 delay 1 second @14:30:26.50 AFV.select :holdingCoil @14:30:26.59 delay 1 second @14:30:27.60 AS.reconfigure ASnorm @14:30:27.63 AS.seek 0.000ml @14:31:19.08 Setting up PCR for ENTem -- writing /var/log/esp/ENTem14mar0814h195ml.pcr @14:31:19.09 Building PCR Slug from ASV with MM2 mix, PP3 primer @14:31:19.10 Priming with carrier @14:31:19.11 ATV.dial ARV @14:31:19.71 ARV.dial :carrier @14:31:20.01 AS.seek 0.500ml @14:31:45.89 AFV.select :carrierSolution @14:31:45.98 delay 2 seconds @14:31:47.99 AS.reconfigure ASconfig @14:31:48.02 AS.seek 1.000ml @14:32:17.45 delay 1 second @14:32:18.46 AFV.select :holdingCoil @14:32:18.55 delay 1 second @14:32:19.56 ATV.dial :waste @14:32:20.90 AS.reconfigure ASnorm @14:32:20.93 AS.seek empty @14:33:12.92 AFV.select :carrierSolution @14:33:13.02 delay 2 seconds @14:33:15.03 AS.reconfigure ASconfig @14:33:15.11 AS.seek 0.500ml @14:33:44.57 delay 1 second @14:33:45.58 AFV.select :holdingCoil @14:33:45.67 Building Slug @14:33:45.68 ATV.dial :ARV @14:33:46.92 delay 2 seconds @14:33:48.93 ARV.dial :air1 @14:33:49.26 delay 2 seconds @14:33:51.32 AS.reconfigure ASslow2 @14:33:51.35 AS.seek 0.506ml @14:33:57.68 delay 2 seconds @14:33:59.69 ARV.dial :MM2 @14:34:01.00 delay 2 seconds @14:34:03.01 Adding MM2 @14:34:03.07 AS.seek 0.524ml @14:34:21.59 delay 25 seconds @14:34:46.60 ARV.dial :PP3 @14:34:46.92 Adding PP3 @14:34:46.93 delay 2 seconds @14:34:48.99 AS.seek 0.530ml @14:34:55.45 delay 10 seconds @14:35:05.46 ARV.dial :air2 @14:35:05.88 adding ARV air @14:35:05.89 delay 2 seconds @14:35:07.95 AS.seek 0.536ml @14:35:14.23 delay 5 seconds @14:35:19.24 ARV.dial :carrier @14:35:20.27 delay 7 seconds @14:35:27.33 AS.seek 0.594ml @14:36:26.58 delay 5 seconds @14:36:31.59 ATV.dial :ASV @14:36:31.91 delay 2 seconds @14:36:33.97 AS.seek 0.600ml @14:36:40.24 delay 4 seconds @14:36:44.25 ATV.dial ARV @14:36:44.58 delay 2 seconds @14:36:46.59 ARV.dial :carrier @14:36:46.60 delay 2 seconds @14:36:48.65 AS.seek 0.650ml @14:37:40.07 delay 4 seconds @14:37:44.08 ATV.dial PCR @14:37:44.58 ASV.dial :waste1 @14:37:44.59 ARV.dial :air1 @14:37:45.09 Positioning Slug by 0.324ml @14:37:45.15 AS.seek 0.326ml @14:43:15.58 delay 5 seconds @14:43:20.59 ATV.dial ARV @14:43:21.19 ASV.dial :PCRlock @14:43:21.83 delay 5 seconds @14:43:26.84 Running ENTem ProfileEM60C thermocycling @14:43:26.85 PCR.program ProfileEM60C @14:43:26.96 ProfileEM60C should complete in < 2:11:38 @14:43:26.97 PCR.run 2:49:32.5 @16:40:06.59 PCR.off @16:40:07.13 PCR for ENTem completed -- closed /var/log/esp/ENTem14mar0814h195ml.pcr @16:40:07.18 Cleaning PCR @16:40:07.19 AFV.select :carrierSolution @16:40:07.28 delay 2 seconds @16:40:09.29 AS.reconfigure ASconfig @16:40:09.32 AS.seek 0.500ml @16:40:19.74 delay 1 second @16:40:20.75 AFV.select :holdingCoil @16:40:20.84 ASV.dial :waste1 @16:40:21.52 ATV.dial :air3 @16:40:22.54 AS.reconfigure ASslow1 @16:40:22.57 AS.seek 0.510ml @16:40:24.88 delay 2 seconds @16:40:26.88 ATV.dial :bleach @16:40:27.26 AS.seek 0.810ml @16:41:28.66 delay 3 seconds @16:41:31.67 ATV.dial :air3 @16:41:31.99 delay 3 seconds @16:41:35.05 AS.seek 0.835ml @16:41:40.38 delay 3 seconds @16:41:43.39 ATV.dial :bleach @16:41:43.77 AS.seek 0.820ml @16:41:47.14 delay 3 seconds @16:41:50.15 ARV.dial :waste @16:41:50.74 delay 3 seconds @16:41:53.75 ATV.dial :ARV @16:41:54.60 AS.reconfigure ASconfig @16:41:54.63 AS.seek 0.470ml @16:42:15.30 AFV.select :carrierSolution @16:42:15.39 delay 2 seconds @16:42:17.40 AS.seek 1.000ml @16:42:48.63 delay 1 second @16:42:49.64 AFV.select :holdingCoil @16:42:49.73 delay 1 second @16:42:50.74 AS.reconfigure ASfast @16:42:50.77 AS.seek 0.000ml @16:43:18.52 AFV.select :carrierSolution @16:43:18.62 delay 2 seconds @16:43:20.63 AS.reconfigure ASconfig @16:43:20.66 AS.seek 1.000ml @16:44:19.25 delay 1 second @16:44:20.26 AFV.select :holdingCoil @16:44:20.35 delay 1 second @16:44:21.36 AS.reconfigure ASfast @16:44:21.39 AS.seek 0.000ml @16:44:49.12 AFV.select :carrierSolution @16:44:49.22 delay 2 seconds @16:44:51.23 AS.reconfigure ASconfig @16:44:51.26 AS.seek 1.000ml @16:45:49.87 delay 1 second @16:45:50.88 AFV.select :holdingCoil @16:45:50.97 delay 1 second @16:45:51.98 AS.reconfigure ASfast @16:45:52.01 AS.seek 0.000ml @16:46:19.76 ARV.dial between carrier and air1 @16:46:20.17 ATV.dial :air3 @16:46:21.18 AS.reconfigure ASslow1 @16:46:21.22 AS.seek 0.010ml @16:46:23.56 delay 2 seconds @16:46:25.57 ATV.dial :bleach @16:46:25.95 AS.seek 0.810ml @16:49:09.36 delay 3 seconds @16:49:12.37 ATV.dial :air3 @16:49:12.79 delay 3 seconds @16:49:15.85 AS.seek 0.835ml @16:49:21.19 delay 3 seconds @16:49:24.20 ATV.dial :bleach @16:49:24.57 AS.seek 0.820ml @16:49:27.88 delay 3 seconds @16:49:30.89 ATV.dial PCR @16:49:31.23 AS.reconfigure ASconfig @16:49:31.26 AS.seek empty @16:50:19.39 PCR.program :bleachProfile @16:50:19.44 PCR program should complete in < 2:45 @16:50:19.45 PCR.run 10:00 @16:53:17.64 PCR.off @16:53:17.67 AFV.select :carrierSolution @16:53:17.76 delay 2 seconds @16:53:19.77 AS.seek 1.000ml @16:54:18.44 delay 1 second @16:54:19.45 AFV.select :holdingCoil @16:54:19.54 delay 1 second @16:54:20.55 AS.reconfigure ASfast @16:54:20.58 AS.seek 0.000ml @16:54:48.33 delay 3 seconds @16:54:51.34 AFV.select :carrierSolution @16:54:51.43 delay 2 seconds @16:54:53.44 AS.reconfigure ASconfig @16:54:53.47 AS.seek 1.000ml @16:55:52.06 delay 1 second @16:55:53.07 AFV.select :holdingCoil @16:55:53.16 delay 1 second @16:55:54.17 AS.reconfigure ASfast @16:55:54.20 AS.seek 0.000ml @16:56:21.91 delay 3 seconds @16:56:24.92 AFV.select :carrierSolution @16:56:25.01 delay 2 seconds @16:56:27.02 AS.reconfigure ASconfig @16:56:27.05 AS.seek 1.000ml @16:57:25.64 delay 1 second @16:57:26.65 AFV.select :holdingCoil @16:57:26.74 delay 1 second @16:57:27.75 AS.reconfigure ASfast @16:57:27.78 AS.seek 0.000ml @16:57:55.47 delay 3 seconds @16:57:58.48 AFV.select :carrierSolution @16:57:58.57 delay 2 seconds @16:58:00.58 AS.reconfigure ASconfig @16:58:00.61 AS.seek 1.000ml @16:58:59.17 delay 1 second @16:59:00.18 AFV.select :holdingCoil @16:59:00.27 delay 1 second @16:59:01.28 AS.reconfigure ASfast @16:59:01.31 AS.seek 0.000ml @16:59:29.01 delay 3 seconds @16:59:32.02 AFV.select :carrierSolution @16:59:32.11 delay 2 seconds @16:59:34.12 AS.reconfigure ASconfig @16:59:34.15 AS.seek 1.000ml @17:00:32.77 delay 1 second @17:00:33.78 AFV.select :holdingCoil @17:00:33.87 delay 1 second @17:00:34.88 AS.reconfigure ASnorm @17:00:34.91 AS.seek 0.000ml @17:01:26.39 Setting up PCR for IPCap -- writing /var/log/esp/IPCap14mar0817h195ml.pcr @17:01:26.40 Building PCR Slug from ASV with MM1 mix, PP2 primer @17:01:26.41 Priming with carrier @17:01:26.42 ATV.dial ARV @17:01:26.91 ARV.dial :carrier @17:01:27.20 AS.seek 0.500ml @17:01:53.11 AFV.select :carrierSolution @17:01:53.22 delay 2 seconds @17:01:55.23 AS.reconfigure ASconfig @17:01:55.27 AS.seek 1.000ml @17:02:24.72 delay 1 second @17:02:25.73 AFV.select :holdingCoil @17:02:25.83 delay 1 second @17:02:26.84 ATV.dial :waste @17:02:28.19 AS.reconfigure ASnorm @17:02:28.22 AS.seek empty @17:03:19.67 AFV.select :carrierSolution @17:03:19.77 delay 2 seconds @17:03:21.78 AS.reconfigure ASconfig @17:03:21.81 AS.seek 0.500ml @17:03:51.30 delay 1 second @17:03:52.31 AFV.select :holdingCoil @17:03:52.40 Building Slug @17:03:52.41 ATV.dial :ARV @17:03:53.64 delay 2 seconds @17:03:55.65 ARV.dial :air1 @17:03:55.97 delay 2 seconds @17:03:58.04 AS.reconfigure ASslow2 @17:03:58.07 AS.seek 0.506ml @17:04:04.48 delay 2 seconds @17:04:06.49 ARV.dial :MM1 @17:04:06.97 delay 2 seconds @17:04:08.98 Adding MM1 @17:04:09.04 AS.seek 0.524ml @17:04:27.55 delay 25 seconds @17:04:52.56 ARV.dial :PP2 @17:04:52.89 Adding PP2 @17:04:52.91 delay 2 seconds @17:04:54.96 AS.seek 0.530ml @17:05:01.19 delay 10 seconds @17:05:11.20 ARV.dial :air2 @17:05:11.62 adding ARV air @17:05:11.63 delay 2 seconds @17:05:13.69 AS.seek 0.536ml @17:05:19.97 delay 5 seconds @17:05:24.98 ARV.dial :carrier @17:05:26.00 delay 7 seconds @17:05:33.06 AS.seek 0.594ml @17:06:32.40 delay 5 seconds @17:06:37.41 ATV.dial :ASV @17:06:37.73 delay 2 seconds @17:06:39.79 AS.seek 0.600ml @17:06:46.24 delay 4 seconds @17:06:50.25 ATV.dial ARV @17:06:50.57 delay 2 seconds @17:06:52.58 ARV.dial :carrier @17:06:52.59 delay 2 seconds @17:06:54.64 AS.seek 0.650ml @17:07:45.96 delay 4 seconds @17:07:49.97 ATV.dial PCR @17:07:50.46 ASV.dial :waste1 @17:07:50.47 ARV.dial :air1 @17:07:50.82 Positioning Slug by 0.324ml @17:07:50.87 AS.seek 0.326ml @17:13:21.74 delay 5 seconds @17:13:26.75 ATV.dial ARV @17:13:27.26 ASV.dial :PCRlock @17:13:27.60 delay 5 seconds @17:13:32.61 Running IPCap Profile59C thermocycling @17:13:32.62 PCR.program Profile59C @17:13:32.72 Profile59C should complete in < 1:39:08 @17:13:32.73 PCR.run 2:08:55 @18:38:00.76 PCR.off @18:38:01.30 PCR for IPCap completed -- closed /var/log/esp/IPCap14mar0817h195ml.pcr @18:38:01.35 Cleaning PCR AFV.select :carrierSolution @18:38:01.44 delay 2 seconds @18:38:03.45 AS.reconfigure ASconfig @18:38:03.48 AS.seek 0.500ml @18:38:13.89 delay 1 second @18:38:14.90 AFV.select :holdingCoil @18:38:14.99 ASV.dial :waste1 @18:38:15.48 ATV.dial :air3 @18:38:16.42 AS.reconfigure ASslow1 @18:38:16.45 AS.seek 0.510ml @18:38:18.73 delay 2 seconds @18:38:20.74 ATV.dial :bleach @18:38:21.12 AS.seek 0.810ml @18:39:22.56 delay 3 seconds @18:39:25.57 ATV.dial :air3 @18:39:25.90 delay 3 seconds @18:39:28.96 AS.seek 0.835ml @18:39:34.29 delay 3 seconds @18:39:37.30 ATV.dial :bleach @18:39:37.68 AS.seek 0.820ml @18:39:40.99 delay 3 seconds @18:39:44.00 ARV.dial :waste @18:39:44.49 delay 3 seconds @18:39:47.50 ATV.dial :ARV @18:39:48.22 AS.reconfigure ASconfig @18:39:48.26 AS.seek 0.470ml @18:40:08.94 AFV.select :carrierSolution @18:40:09.03 delay 2 seconds @18:40:11.04 AS.seek 1.000ml @18:40:42.21 delay 1 second @18:40:43.22 AFV.select :holdingCoil @18:40:43.31 delay 1 second @18:40:44.32 AS.reconfigure ASfast @18:40:44.35 AS.seek 0.000ml @18:41:12.10 AFV.select :carrierSolution @18:41:12.20 delay 2 seconds @18:41:14.21 AS.reconfigure ASconfig @18:41:14.24 AS.seek 1.000ml @18:42:12.83 delay 1 second @18:42:13.84 AFV.select :holdingCoil @18:42:13.93 delay 1 second @18:42:14.94 AS.reconfigure ASfast @18:42:14.97 AS.seek 0.000ml @18:42:42.72 AFV.select :carrierSolution @18:42:42.82 delay 2 seconds @18:42:44.83 AS.reconfigure ASconfig @18:42:44.86 AS.seek 1.000ml @18:43:43.44 delay 1 second @18:43:44.45 AFV.select :holdingCoil @18:43:44.54 delay 1 second @18:43:45.55 AS.reconfigure ASfast @18:43:45.58 AS.seek 0.000ml @18:44:13.26 ARV.dial between carrier and air1 @18:44:13.67 ATV.dial :air3 @18:44:14.59 AS.reconfigure ASslow1 @18:44:14.63 AS.seek 0.010ml @18:44:16.97 delay 2 seconds @18:44:18.98 ATV.dial :bleach @18:44:19.36 AS.seek 0.810ml @18:47:02.76 delay 3 seconds @18:47:05.77 ATV.dial :air3 @18:47:06.10 delay 3 seconds @18:47:09.16 AS.seek 0.835ml @18:47:14.54 delay 3 seconds @18:47:17.55 ATV.dial :bleach @18:47:17.93 AS.seek 0.820ml @18:47:21.20 delay 3 seconds @18:47:24.21 ATV.dial PCR @18:47:24.55 AS.reconfigure ASconfig @18:47:24.58 AS.seek empty @18:48:12.71 PCR.program :bleachProfile @18:48:12.77 PCR program should complete in < 2:45 @18:48:12.78 PCR.run 10:00 @18:51:10.97 PCR.off @18:51:11.00 AFV.select :carrierSolution @18:51:11.09 delay 2 seconds @18:51:13.10 AS.seek 1.000ml @18:52:11.75 delay 1 second @18:52:12.76 AFV.select :holdingCoil @18:52:12.85 delay 1 second @18:52:13.86 AS.reconfigure ASfast @18:52:13.89 AS.seek 0.000ml @18:52:41.58 delay 3 seconds @18:52:44.59 AFV.select :carrierSolution @18:52:44.68 delay 2 seconds @18:52:46.69 AS.reconfigure ASconfig @18:52:46.72 AS.seek 1.000ml @18:53:45.93 delay 1 second @18:53:46.94 AFV.select :holdingCoil @18:53:47.03 delay 1 second @18:53:48.04 AS.reconfigure ASfast @18:53:48.07 AS.seek 0.000ml @18:54:15.82 delay 3 seconds @18:54:18.83 AFV.select :carrierSolution @18:54:18.92 delay 2 seconds @18:54:20.93 AS.reconfigure ASconfig @18:54:20.97 AS.seek 1.000ml @18:55:19.56 delay 1 second @18:55:20.57 AFV.select :holdingCoil @18:55:20.66 delay 1 second @18:55:21.67 AS.reconfigure ASfast @18:55:21.70 AS.seek 0.000ml @18:55:49.44 delay 3 seconds @18:55:52.45 AFV.select :carrierSolution @18:55:52.54 delay 2 seconds @18:55:54.55 AS.reconfigure ASconfig @18:55:54.58 AS.seek 1.000ml @18:56:53.19 delay 1 second @18:56:54.20 AFV.select :holdingCoil @18:56:54.29 delay 1 second @18:56:55.30 AS.reconfigure ASfast @18:56:55.34 AS.seek 0.000ml @18:57:23.08 delay 3 seconds @18:57:26.09 AFV.select :carrierSolution @18:57:26.18 delay 2 seconds @18:57:28.19 AS.reconfigure ASconfig @18:57:28.22 AS.seek 1.000ml @18:58:26.79 delay 1 second @18:58:27.80 AFV.select :holdingCoil @18:58:27.89 delay 1 second @18:58:28.90 AS.reconfigure ASnorm @18:58:28.93 AS.seek 0.000ml @18:59:20.38 Setting up PCR for IPCem -- writing /var/log/esp/IPCem14mar0818h195ml.pcr @18:59:20.39 Building PCR Slug from ASV with MM3 mix, PP5 primer @18:59:20.40 Priming with carrier @18:59:20.41 ATV.dial ARV @18:59:20.90 ARV.dial :carrier @18:59:21.18 AS.seek 0.500ml @18:59:47.10 AFV.select :carrierSolution @18:59:47.20 delay 2 seconds @18:59:49.21 AS.reconfigure ASconfig @18:59:49.24 AS.seek 1.000ml @19:00:18.66 delay 1 second @19:00:19.67 AFV.select :holdingCoil @19:00:19.77 delay 1 second @19:00:20.78 ATV.dial :waste @19:00:22.02 AS.reconfigure ASnorm @19:00:22.05 AS.seek empty @19:01:13.52 AFV.select :carrierSolution @19:01:13.62 delay 2 seconds @19:01:15.63 AS.reconfigure ASconfig @19:01:15.66 AS.seek 0.500ml @19:01:45.15 delay 1 second @19:01:46.16 AFV.select :holdingCoil @19:01:46.25 Building Slug @19:01:46.26 ATV.dial :ARV @19:01:47.58 delay 2 seconds @19:01:49.59 ARV.dial :air1 @19:01:49.91 delay 2 seconds @19:01:51.97 AS.reconfigure ASslow2 @19:01:52.00 AS.seek 0.506ml @19:01:58.58 delay 2 seconds @19:02:00.59 ARV.dial :MM3 @19:02:01.46 delay 2 seconds @19:02:03.47 Adding MM3 @19:02:03.52 AS.seek 0.524ml @19:02:22.22 delay 25 seconds @19:02:47.23 ARV.dial :PP5 @19:02:47.53 Adding PP5 @19:02:47.54 delay 2 seconds @19:02:49.60 AS.seek 0.530ml @19:02:56.14 delay 10 seconds @19:03:06.15 ARV.dial :air3 @19:03:06.46 adding ARV air @19:03:06.47 delay 2 seconds @19:03:08.53 AS.seek 0.536ml @19:03:14.88 delay 5 seconds @19:03:19.89 ARV.dial :carrier @19:03:20.92 delay 7 seconds @19:03:27.98 AS.seek 0.594ml @19:04:27.45 delay 5 seconds @19:04:32.46 ATV.dial :ASV @19:04:32.78 delay 2 seconds @19:04:34.84 AS.seek 0.600ml @19:04:41.33 delay 4 seconds @19:04:45.34 ATV.dial ARV @19:04:45.67 delay 2 seconds @19:04:47.68 ARV.dial :carrier @19:04:47.69 delay 2 seconds @19:04:49.74 AS.seek 0.650ml @19:05:40.89 delay 4 seconds @19:05:44.90 ATV.dial PCR @19:05:45.40 ASV.dial :waste1 @19:05:45.41 ARV.dial :air1 @19:05:45.74 Positioning Slug by 0.324ml @19:05:45.79 AS.seek 0.326ml @19:11:16.32 delay 5 seconds @19:11:21.33 ATV.dial ARV @19:11:21.84 ASV.dial :PCRlock @19:11:22.32 delay 5 seconds @19:11:27.33 Running IPCem ProfileEM59C thermocycling @19:11:27.34 PCR.program ProfileEM59C @19:11:27.45 ProfileEM59C should complete in < 2:11:38 @19:11:27.46 PCR.run 2:49:32.5 @21:09:01.15 PCR.off @21:09:01.68 PCR for IPCem completed -- closed /var/log/esp/IPCem14mar0818h195ml.pcr @21:09:01.70 Full MFB Decontamination Decon extraction column and decon AIV @21:09:01.72 SPE.hold 2:46:40,55.0C @21:09:01.73 ASV.dial :SPEdelivery @21:09:02.69 delay 20 seconds @21:09:22.70 AFV.select :carrierSolution @21:09:22.79 delay 2 seconds @21:09:24.80 AS.reconfigure ASconfig @21:09:24.83 AS.seek 0.500ml @21:09:35.25 delay 1 second @21:09:36.26 AFV.select :holdingCoil @21:09:36.35 ATV.dial :air1 @21:09:37.85 AS.reconfigure ASslow1 @21:09:37.88 AS.seek 0.520ml @21:09:42.22 ATV.dial :ASV @21:09:43.49 ASV.dial :SPErecovery @21:09:44.19 AS.seek 0.620ml @21:10:04.87 ATV.dial :waste @21:10:06.34 AS.reconfigure ASnorm @21:10:06.37 AS.seek empty @21:10:38.42 AFV.select :holdingCoil @21:10:38.43 ATV.dial :waste @21:10:38.44 AS.reconfigure ASconfig @21:10:38.47 AS.seek 0.000ml @21:10:38.78 AFV.select :carrierSolution @21:10:38.87 delay 2 seconds @21:10:40.88 AS.seek 0.500ml @21:11:10.28 delay 1 second @21:11:11.29 AFV.select :holdingCoil @21:11:11.38 ATV.dial :air3 @21:11:12.05 AS.reconfigure ASslow1 @21:11:12.08 AS.seek 0.520ml @21:11:16.42 ATV.dial :air3 @21:11:16.48 AS.seek 0.530ml @21:11:18.76 delay 2 seconds @21:11:20.77 ATV.dial :bleach @21:11:21.25 AS.seek 0.710ml @21:11:58.20 delay 3 seconds @21:12:01.21 ATV.dial :air3 @21:12:01.54 delay 3 seconds @21:12:04.60 AS.seek 0.735ml @21:12:09.90 delay 3 seconds @21:12:12.91 ATV.dial :bleach @21:12:13.31 AS.seek 0.720ml @21:12:16.60 delay 3 seconds @21:12:19.61 ATV.dial ASV @21:12:20.48 ASV.dial :SPErecovery @21:12:20.54 AS.seek 0.365ml @21:13:33.16 ATV.dial :waste @21:13:34.61 AS.reconfigure ASnorm @21:13:34.64 AS.seek empty @21:13:53.61 ATV.dial :air3 @21:13:54.26 AS.reconfigure ASslow1 @21:13:54.29 AS.seek 0.009ml @21:13:56.61 delay 2 seconds @21:13:58.62 ATV.dial :bleach @21:13:58.99 AS.seek 0.579ml @21:15:55.49 delay 3 seconds @21:15:58.50 ATV.dial :air3 @21:15:58.83 delay 3 seconds @21:16:01.89 AS.seek 0.604ml @21:16:07.30 delay 3 seconds @21:16:10.31 ATV.dial :bleach @21:16:11.31 AS.seek 0.589ml @21:16:14.64 delay 3 seconds @21:16:17.65 ATV.dial :ASV @21:16:18.63 ASV.dial :wasteR @21:16:19.40 AS.seek 0.000ml @21:18:19.82 delay 1:00 @21:19:19.83 ATV.dial ASV @21:19:19.84 AFV.select :carrierSolution @21:19:19.93 delay 2 seconds @21:19:21.94 AS.reconfigure ASconfig @21:19:21.97 AS.seek 1.000ml @21:20:20.59 delay 1 second @21:20:21.60 AFV.select :holdingCoil @21:20:21.69 delay 1 second @21:20:22.70 ASV.dial :wasteR @21:20:22.71 AS.reconfigure ASslow1 @21:20:22.74 AS.seek 0.000ml @21:23:47.00 delay 10 seconds @21:23:57.01 ATV.dial :air3 @21:23:58.13 AS.seek 0.010ml @21:24:00.49 delay 2 seconds @21:24:02.50 ATV.dial :bleach @21:24:02.89 AS.seek 0.580ml @21:25:59.37 delay 3 seconds @21:26:02.38 ATV.dial :air3 @21:26:02.72 delay 3 seconds @21:26:05.78 AS.seek 0.605ml @21:26:11.11 delay 3 seconds @21:26:14.12 ATV.dial :bleach @21:26:14.61 AS.seek 0.590ml @21:26:17.92 delay 3 seconds @21:26:20.93 ATV.dial ASV @21:26:21.81 ASV.dial :SPEdelivery @21:26:22.29 AS.seek 0.000ml @21:28:22.88 delay 1:00 @21:29:22.89 AFV.select :carrierSolution @21:29:22.98 delay 2 seconds @21:29:24.99 AS.reconfigure ASconfig @21:29:25.03 AS.seek 1.000ml @21:30:23.64 delay 1 second @21:30:24.65 AFV.select :holdingCoil @21:30:24.74 delay 1 second @21:30:25.75 ATV.dial ASV @21:30:25.76 ASV.dial :SPEdelivery @21:30:25.77 AS.reconfigure ASslow1 @21:30:25.80 AS.seek 0.000ml @21:33:50.01 delay 10 seconds @21:34:00.02 ATV.dial :air2 @21:34:00.84 AS.reconfigure ASslow0 @21:34:00.87 AS.seek 0.800ml @21:35:22.74 delay 3 seconds @21:35:25.75 ATV.dial :ASV @21:35:26.55 ASV.dial :SPEdelivery @21:35:26.57 AS.reconfigure ASslow1 @21:35:26.60 AS.seek 0.030ml @21:38:03.89 delay 20 seconds @21:38:23.90 ATV.dial :waste,via => air3 @21:38:25.35 AS.reconfigure ASconfig @21:38:25.38 AS.seek empty @21:38:27.47 SPE.coast @21:38:27.55 Removing Bleach from Mixing Coil @21:38:27.56 AFV.select :carrierSolution @21:38:27.65 delay 2 seconds @21:38:29.66 AS.seek 0.500ml @21:38:59.17 delay 1 second @21:39:00.18 AFV.select :holdingCoil @21:39:00.43 ATV.dial :ASV @21:39:01.87 ASV.dial :SPErecovery @21:39:02.55 AS.reconfigure ASslow1 @21:39:02.59 AS.seek 0.730ml @21:39:49.73 ASV.dial :wasteD @21:39:50.35 AS.reconfigure ASnorm @21:39:50.38 AS.seek 0.000ml @21:40:28.01 AFV.select :carrierSolution @21:40:28.11 delay 2 seconds @21:40:30.12 AS.reconfigure ASconfig @21:40:30.15 AS.seek 0.500ml @21:40:59.59 delay 1 second @21:41:00.60 AFV.select :holdingCoil @21:41:00.69 ATV.dial ASV @21:41:00.70 ASV.dial :SPErecovery @21:41:01.40 AS.reconfigure ASslow0 @21:41:01.43 AS.seek 0.300ml @21:41:22.12 ATV.dial :air2 @21:41:22.86 AS.reconfigure ASslow1 @21:41:22.90 AS.seek 0.320ml @21:41:27.19 ATV.dial ASV @21:41:27.94 AS.reconfigure ASslow0 @21:41:27.98 AS.seek 0.570ml @21:41:53.80 ASV.dial :wasteD @21:41:54.73 AS.reconfigure ASnorm @21:41:54.76 AS.seek 0.000ml @21:42:24.24 AFV.select :carrierSolution @21:42:24.34 delay 2 seconds @21:42:26.35 AS.reconfigure ASconfig @21:42:26.38 AS.seek 0.500ml @21:42:55.82 delay 1 second @21:42:56.83 AFV.select :holdingCoil @21:42:56.92 ATV.dial ASV @21:42:56.93 ASV.dial :SPErecovery @21:42:57.46 AS.reconfigure ASslow0 @21:42:57.49 AS.seek 0.300ml @21:43:18.17 ATV.dial :air2 @21:43:18.92 AS.reconfigure ASslow1 @21:43:18.96 AS.seek 0.320ml @21:43:23.22 ATV.dial ASV @21:43:23.96 AS.reconfigure ASslow0 @21:43:24.00 AS.seek 0.570ml @21:43:49.83 ASV.dial :wasteD @21:43:50.48 AS.reconfigure ASnorm @21:43:50.51 AS.seek 0.000ml @21:44:20.00 ATV.dial :air2 @21:44:20.72 AS.reconfigure ASslow0 @21:44:20.75 AS.seek 0.300ml @21:44:51.71 ATV.dial :ASV @21:44:52.42 ASV.dial :wasteD @21:44:52.44 AS.reconfigure ASconfig @21:44:52.47 AS.seek 0.050ml @21:45:07.35 ATV.dial :waste @21:45:08.80 AS.seek 0.000ml @21:45:12.01 ASV.dial :SPEdelivery @21:45:13.01 deconLysateINT @21:45:13.02 ATV.dial :air3 @21:45:14.15 AS.reconfigure ASslow1 @21:45:14.19 AS.seek 0.010ml @21:45:16.54 delay 2 seconds @21:45:18.55 ATV.dial :bleach @21:45:19.03 AS.seek 0.960ml @21:48:33.03 delay 3 seconds @21:48:36.04 ATV.dial :air3 @21:48:36.36 delay 3 seconds @21:48:39.42 AS.seek 0.985ml @21:48:44.74 delay 3 seconds @21:48:47.75 ATV.dial :bleach @21:48:48.24 AS.seek 0.970ml @21:48:51.56 delay 3 seconds @21:48:54.57 ATV.dial :AIV @21:48:55.64 AIV.dial :coilToMFB @21:48:55.65 AWV.select :waste @21:48:55.66 AFV.select :carrierSolution @21:48:55.75 delay 2 seconds @21:48:57.76 AS.reconfigure ASconfig @21:48:57.79 AS.seek 1.000ml @21:48:59.86 delay 1 second @21:49:00.87 AFV.select :holdingCoil @21:49:00.96 delay 1 second @21:49:01.97 AS.reconfigure ASslow0 @21:49:02.00 AS.seek empty @21:50:44.39 AFV.select :carrierSolution @21:50:44.49 delay 2 seconds @21:50:46.50 AS.reconfigure ASconfig @21:50:46.54 AS.seek 1.000ml @21:51:45.15 delay 1 second @21:51:46.16 AFV.select :holdingCoil @21:51:46.25 delay 1 second @21:51:47.26 AS.reconfigure ASslow0 @21:51:47.29 AS.seek empty @21:53:29.71 AFV.select :carrierSolution @21:53:29.81 delay 2 seconds @21:53:31.82 AS.reconfigure ASconfig @21:53:31.86 AS.seek 1.000ml @21:54:30.54 delay 1 second @21:54:31.55 AFV.select :holdingCoil @21:54:31.64 delay 1 second @21:54:32.65 AS.reconfigure ASslow0 @21:54:32.68 AS.seek empty @21:56:15.06 ATV.dial :air2 @21:56:15.63 AS.reconfigure ASslow1 @21:56:15.66 AS.seek 0.009ml @21:56:18.00 AS.reconfigure ASslow0 @21:56:18.04 AS.seek 1.000ml @21:57:59.36 ATV.dial AIV @21:57:59.90 AS.seek 0.020ml @21:59:40.18 ATV.dial :waste @21:59:41.46 AS.seek 0.000ml @21:59:43.79 AWV.select :air @21:59:43.94 Cleaning PCR @21:59:43.95 AFV.select :carrierSolution @21:59:44.04 delay 2 seconds @21:59:46.05 AS.reconfigure ASconfig @21:59:46.08 AS.seek 0.500ml @22:00:15.56 delay 1 second @22:00:16.57 AFV.select :holdingCoil @22:00:16.66 ASV.dial :waste1 @22:00:16.99 ATV.dial :air3 @22:00:17.55 AS.reconfigure ASslow1 @22:00:17.59 AS.seek 0.510ml @22:00:19.88 delay 2 seconds @22:00:21.89 ATV.dial :bleach @22:00:22.36 AS.seek 0.810ml @22:01:23.77 delay 3 seconds @22:01:26.78 ATV.dial :air3 @22:01:27.11 delay 3 seconds @22:01:30.17 AS.seek 0.835ml @22:01:35.50 delay 3 seconds @22:01:38.51 ATV.dial :bleach @22:01:38.90 AS.seek 0.820ml @22:01:42.22 delay 3 seconds @22:01:45.23 ARV.dial :waste @22:01:45.74 delay 3 seconds @22:01:48.75 ATV.dial :ARV @22:01:49.48 AS.reconfigure ASconfig @22:01:49.52 AS.seek 0.470ml @22:02:10.20 AFV.select :carrierSolution @22:02:10.29 delay 2 seconds @22:02:12.30 AS.seek 1.000ml @22:02:43.48 delay 1 second @22:02:44.49 AFV.select :holdingCoil @22:02:44.58 delay 1 second @22:02:45.59 AS.reconfigure ASfast @22:02:45.62 AS.seek 0.000ml @22:03:13.33 AFV.select :carrierSolution @22:03:13.43 delay 2 seconds @22:03:15.44 AS.reconfigure ASconfig @22:03:15.47 AS.seek 1.000ml @22:04:14.06 delay 1 second @22:04:15.07 AFV.select :holdingCoil @22:04:15.16 delay 1 second @22:04:16.17 AS.reconfigure ASfast @22:04:16.20 AS.seek 0.000ml @22:04:43.90 AFV.select :carrierSolution @22:04:44.00 delay 2 seconds @22:04:46.01 AS.reconfigure ASconfig @22:04:46.04 AS.seek 1.000ml @22:05:44.65 delay 1 second @22:05:45.66 AFV.select :holdingCoil @22:05:45.75 delay 1 second @22:05:46.76 AS.reconfigure ASfast @22:05:46.79 AS.seek 0.000ml @22:06:14.52 ARV.dial between carrier and air1 @22:06:14.93 ATV.dial :air3 @22:06:15.94 AS.reconfigure ASslow1 @22:06:15.98 AS.seek 0.010ml @22:06:18.32 delay 2 seconds @22:06:20.33 ATV.dial :bleach @22:06:20.82 AS.seek 0.810ml @22:09:04.24 delay 3 seconds @22:09:07.25 ATV.dial :air3 @22:09:07.58 delay 3 seconds @22:09:10.64 AS.seek 0.835ml @22:09:15.97 delay 3 seconds @22:09:18.98 ATV.dial :bleach @22:09:19.36 AS.seek 0.820ml @22:09:22.85 delay 3 seconds @22:09:25.86 ATV.dial PCR @22:09:26.18 AS.reconfigure ASconfig @22:09:26.21 AS.seek empty @22:10:14.33 PCR.program :bleachProfile @22:10:14.38 PCR program should complete in < 2:45 @22:10:14.39 PCR.run 10:00 @22:13:16.16 PCR.off @22:13:16.19 AFV.select :carrierSolution @22:13:16.28 delay 2 seconds @22:13:18.29 AS.seek 1.000ml @22:14:16.94 delay 1 second @22:14:17.95 AFV.select :holdingCoil @22:14:18.04 delay 1 second @22:14:19.05 AS.reconfigure ASfast @22:14:19.08 AS.seek 0.000ml @22:14:46.77 delay 3 seconds @22:14:49.78 AFV.select :carrierSolution @22:14:49.87 delay 2 seconds @22:14:51.88 AS.reconfigure ASconfig @22:14:51.91 AS.seek 1.000ml @22:15:50.52 delay 1 second @22:15:51.53 AFV.select :holdingCoil @22:15:51.62 delay 1 second @22:15:52.63 AS.reconfigure ASfast @22:15:52.66 AS.seek 0.000ml @22:16:20.39 delay 3 seconds @22:16:23.40 AFV.select :carrierSolution @22:16:23.49 delay 2 seconds @22:16:25.50 AS.reconfigure ASconfig @22:16:25.54 AS.seek 1.000ml @22:17:24.14 delay 1 second @22:17:25.15 AFV.select :holdingCoil @22:17:25.24 delay 1 second @22:17:26.25 AS.reconfigure ASfast @22:17:26.28 AS.seek 0.000ml @22:17:54.03 delay 3 seconds @22:17:57.04 AFV.select :carrierSolution @22:17:57.13 delay 2 seconds @22:17:59.14 AS.reconfigure ASconfig @22:17:59.17 AS.seek 1.000ml @22:18:57.80 delay 1 second @22:18:58.81 AFV.select :holdingCoil @22:18:58.90 delay 1 second @22:18:59.91 AS.reconfigure ASfast @22:18:59.94 AS.seek 0.000ml @22:19:27.65 delay 3 seconds @22:19:30.66 AFV.select :carrierSolution @22:19:30.75 delay 2 seconds @22:19:32.76 AS.reconfigure ASconfig @22:19:32.79 AS.seek 1.000ml @22:20:31.38 delay 1 second @22:20:32.39 AFV.select :holdingCoil @22:20:32.48 delay 1 second @22:20:33.49 AS.reconfigure ASnorm @22:20:33.52 AS.seek 0.000ml @22:21:24.99 AFV.select :carrierSolution @22:21:25.08 delay 2 seconds @22:21:27.09 AS.reconfigure ASconfig @22:21:27.12 AS.seek 1.000ml @22:22:25.74 delay 1 second @22:22:26.75 AFV.select :holdingCoil @22:22:26.84 delay 1 second @22:22:27.85 ATV.dial :waste @22:22:28.75 AS.reconfigure ASnorm @22:22:28.78 AS.seek 0.500ml @22:22:54.70 ATV.dial :waste2 @22:22:55.89 AS.seek 0.000ml @22:23:21.81
Cleaning PCR AFV.select :carrierSolution @22:23:21.90 delay 2 seconds @22:23:23.91 AS.reconfigure ASconfig @22:23:23.94 AS.seek 0.500ml @22:23:53.37 delay 1 second @22:23:54.38 AFV.select :holdingCoil @22:23:54.47 ASV.dial :waste1 @22:23:54.48 ATV.dial :air3 @22:23:55.97 AS.reconfigure ASslow1 @22:23:56.00 AS.seek 0.510ml @22:23:58.20 delay 2 seconds @22:24:00.21 ATV.dial :bleach @22:24:00.58 AS.seek 0.810ml @22:25:02.01 delay 3 seconds @22:25:05.03 ATV.dial :air3 @22:25:05.37 delay 3 seconds @22:25:08.42 AS.seek 0.835ml @22:25:13.76 delay 3 seconds @22:25:16.77 ATV.dial :bleach @22:25:17.14 AS.seek 0.820ml @22:25:20.45 delay 3 seconds @22:25:23.46 ARV.dial :waste @22:25:23.87 delay 3 seconds @22:25:26.88 ATV.dial :ARV @22:25:27.64 AS.reconfigure ASconfig @22:25:27.67 AS.seek 0.470ml @22:25:48.34 AFV.select :carrierSolution @22:25:48.43 delay 2 seconds @22:25:50.44 AS.seek 1.000ml @22:26:21.66 delay 1 second @22:26:22.67 AFV.select :holdingCoil @22:26:22.76 delay 1 second @22:26:23.77 AS.reconfigure ASfast @22:26:23.80 AS.seek 0.000ml @22:26:51.51 AFV.select :carrierSolution @22:26:51.60 delay 2 seconds @22:26:53.61 AS.reconfigure ASconfig @22:26:53.64 AS.seek 1.000ml @22:27:52.26 delay 1 second @22:27:53.28 AFV.select :holdingCoil @22:27:53.37 delay 1 second @22:27:54.38 AS.reconfigure ASfast @22:27:54.41 AS.seek 0.000ml @22:28:22.15 AFV.select :carrierSolution @22:28:22.24 delay 2 seconds @22:28:24.25 AS.reconfigure ASconfig @22:28:24.29 AS.seek 1.000ml @22:29:22.89 delay 1 second @22:29:23.91 AFV.select :holdingCoil @22:29:24.00 delay 1 second @22:29:25.01 AS.reconfigure ASfast @22:29:25.05 AS.seek 0.000ml @22:29:52.75 ARV.dial between carrier and air1 @22:29:53.16 ATV.dial :air3 @22:29:54.10 AS.reconfigure ASslow1 @22:29:54.13 AS.seek 0.010ml @22:29:56.47 delay 2 seconds @22:29:58.48 ATV.dial :bleach @22:29:58.97 AS.seek 0.810ml @22:32:42.48 delay 3 seconds @22:32:45.49 ATV.dial :air3 @22:32:45.82 delay 3 seconds @22:32:48.88 AS.seek 0.835ml @22:32:54.26 delay 3 seconds @22:32:57.27 ATV.dial :bleach @22:32:57.65 AS.seek 0.820ml @22:33:00.96 delay 3 seconds @22:33:03.97 ATV.dial PCR @22:33:04.83 AS.reconfigure ASconfig @22:33:04.86 AS.seek empty @22:33:52.96 PCR.program :bleachProfile @22:33:53.02 PCR program should complete in < 2:45 @22:33:53.03 PCR.run 10:00 @22:36:57.27 PCR.off @22:36:57.30 AFV.select :carrierSolution @22:36:57.39 delay 2 seconds @22:36:59.40 AS.seek 1.000ml @22:37:58.09 delay 1 second @22:37:59.10 AFV.select :holdingCoil @22:37:59.19 delay 1 second @22:38:00.20 AS.reconfigure ASfast @22:38:00.24 AS.seek 0.000ml @22:38:27.98 delay 3 seconds @22:38:30.99 AFV.select :carrierSolution @22:38:31.08 delay 2 seconds @22:38:33.09 AS.reconfigure ASconfig @22:38:33.12 AS.seek 1.000ml @22:39:31.73 delay 1 second @22:39:32.75 AFV.select :holdingCoil @22:39:32.84 delay 1 second @22:39:33.85 AS.reconfigure ASfast @22:39:33.88 AS.seek 0.000ml @22:40:01.63 delay 3 seconds @22:40:04.64 AFV.select :carrierSolution @22:40:04.73 delay 2 seconds @22:40:06.74 AS.reconfigure ASconfig @22:40:06.77 AS.seek 1.000ml @22:41:05.38 delay 1 second @22:41:06.40 AFV.select :holdingCoil @22:41:06.49 delay 1 second @22:41:07.50 AS.reconfigure ASfast @22:41:07.54 AS.seek 0.000ml @22:41:35.27 delay 3 seconds @22:41:38.28 AFV.select :carrierSolution @22:41:38.37 delay 2 seconds @22:41:40.38 AS.reconfigure ASconfig @22:41:40.41 AS.seek 1.000ml @22:42:39.00 delay 1 second @22:42:40.01 AFV.select :holdingCoil @22:42:40.10 delay 1 second @22:42:41.11 AS.reconfigure ASfast @22:42:41.14 AS.seek 0.000ml @22:43:08.88 delay 3 seconds @22:43:11.90 AFV.select :carrierSolution @22:43:11.99 delay 2 seconds @22:43:14.00 AS.reconfigure ASconfig @22:43:14.03 AS.seek 1.000ml @22:44:12.62 delay 1 second @22:44:13.63 AFV.select :holdingCoil @22:44:13.72 delay 1 second @22:44:14.73 AS.reconfigure ASnorm @22:44:14.76 AS.seek 0.000ml @22:45:06.20 Completed {wqPCR} begun 08:01:34 @22:45:06.23 Asleep til 09:01PDT09-Mar-14 @22:45:06.24 AS.stop @22:45:06.25
Sleeping until 09:01PDT09-Mar-14 @22:45:06.27 GoodNews.email "Then, in tube 2: wqPCR\n/home/esp/esp2local/mission/14mar05sccwrpDeploy.rb:64",Subject => "Asleep until 09:01PDT09-Mar-14" @22:45:07.99 CC.stop @22:45:08.01 CS.stop @22:45:08.04 PC.stop @22:45:08.07 PS.stop @22:45:08.09 Forearm.stop @22:45:08.12 Elbow.stop @22:45:08.15 SS.stop @22:45:08.22 Sleepy.power :raw,:OFF @22:45:08.23 Sleepy.power :dwarf,:OFF @22:45:08.24 Shutdown @09:01:34.87PDT09-Mar-14
Powering up Dwarves @09:01:39.75 Sleepy.power :raw,:ON @09:01:40.22 SPE.configure SPEconfig @09:01:40.24 AS.configure ASconfig @09:01:40.28 CC.configure CCconfig @09:01:40.31 CS.configure CSconfig @09:01:40.34 PC.configure PCconfig @09:01:40.37 PS.configure PSconfig @09:01:40.40 Forearm.configure LooseForearm @09:01:40.44 Elbow.configure LooseElbow @09:01:40.47 SC.configure SCloose @09:01:40.50 SE.configure SEconfig @09:01:41.02 SS.configure SSconfig @09:01:41.05 CH.configure CHconfig @09:01:41.07 PH.configure PHconfig @09:01:41.10 Valve.reset @09:01:41.26 PCR.off @09:01:41.29 PCR.pidtemp= 50,0.1,0,300,25,25 @09:01:41.32 PCR.caltemp= 6.6661,0.0344,0.0 @09:01:41.44 Intake.select :close @09:01:41.45 Exhaust.select :close @09:01:41.46 CTV.dial :air @09:01:41.50 PSV.dial :air @09:01:41.53
PC.reconfigure PCconfig @09:01:41.59 PC.home.seek open @09:01:41.61 CSV.dial :waste1 @09:01:42.01 PS.reconfigure PSconfig @09:01:42.04 PS.home.seek 0.000ml @09:01:42.08 SSV.dial :spit,avoiding => exhaust @09:01:42.87 CS.reconfigure CSfast @09:01:42.91 SS.reconfigure SSconfig @09:01:43.04 CS.home.seek 0.000ml @09:01:43.06 SS.home.seek 0.00ml @09:01:43.19 CC.reconfigure CCconfig @09:01:43.22 CC.home.seek open @09:01:52.32 PS.coast @09:01:52.40
CC.seek open @09:01:52.42 PC.seek open @09:01:52.57
Forearm.reconfigure LooseForearm @09:01:52.61 Forearm.home.seek retract @09:01:53.35 Hand.grip @09:01:53.37
Elbow.jog -1500 @09:01:53.93 Elbow.reconfigure LooseElbow @09:01:53.97 Elbow.home.seek clear @09:01:58.71 SE.reconfigure SEconfig @09:01:58.74 SE.home.seek down @09:01:59.31 SC.reconfigure SCsloppy @09:01:59.35 SC.home.seek initial @09:02:01.56 Forearm.seek retract @09:02:01.61 Elbow.seek hammer @09:02:02.79 Forearm.reconfigure TightForearm @09:02:02.84 Forearm.seek 2 @09:02:03.89 Forearm.seek retract @09:02:04.97 Forearm.reconfigure LooseForearm @09:02:05.46PDT09-Mar-14 ===> STARTED wqPCR (phase #6) <=== @09:02:05.47 Starting initialPurge @09:02:05.48 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @09:02:05.53 Hand.release @09:02:06.33 Elbow.reconfigure TightElbow @09:02:06.37 Elbow.seek FlushPuck::Garage @09:02:08.15 Elbow.reconfigure LooseElbow @09:02:08.23 Forearm.seek garage @09:02:09.21 Hand.grip @09:02:10.09 Forearm.seek retract @09:02:11.17 Elbow.reconfigure TightElbow @09:02:11.20 Elbow.seek CC @09:02:14.00 Elbow.reconfigure LooseElbow @09:02:14.09 Forearm.reconfigure TightForearm @09:02:14.12 Forearm.seek CC @09:02:15.23 CC.seek closed @09:02:19.00 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.94 @09:02:19.05 Hand.release @09:02:19.79 Forearm.reconfigure LooseForearm @09:02:19.82 Forearm.seek retract @09:02:20.81 Flushing the Collection Valve::Manifold @09:02:20.90 CS Pulling 5ml of flush @09:02:20.91 CSV.dial CSR @09:02:22.17 CSR.select :flush @09:02:22.27 CS.reconfigure CSconfig @09:02:22.31 CS.seek 5.000ml @09:02:35.79 delay 5 seconds @09:02:40.80 CSR.select :air @09:02:40.94 CS.reconfigure CSfast @09:02:40.97 CS.seek 7.500ml @09:02:47.54 CSV.dial :waste @09:02:48.79 CS.reconfigure CSconfig @09:02:48.83 CS.seek 0.000ml @09:03:08.70 Purging external Intake @09:03:08.79 CSV.dial :flush @09:03:09.44 CS.reconfigure CSfast @09:03:09.47 CS.seek 10.000ml @09:03:33.07 delay 5 seconds @09:03:38.09 CSV.dial CBV @09:03:38.11
CBV.dial CSV @09:03:38.82 CTV.dial :intake @09:03:39.57 Intake.select :open @09:03:39.66 CS.seek 0.000ml @09:04:03.92 Intake.select :close @09:04:04.01 CTV.dial :air @09:04:04.74 Flushing upper manifold @09:04:04.83 CSV.dial :flush @09:04:05.72 CS.seek 5.000ml @09:04:18.07 delay 5 seconds @09:04:23.08 CSV.dial :air @09:04:23.56 CS.seek 7.000ml @09:04:28.98 CSV.dial CBV @09:04:29.00
CBV.dial CSV @09:04:30.07 CTV.dial :air @09:04:30.09 CS.seek 0.000ml @09:04:46.85 delay 7 seconds @09:04:53.86 CS.seek 3.000ml @09:05:01.54 CSV.dial :waste @09:05:02.05 CS.seek 0.000ml @09:05:09.93 CSV.dial :flush @09:05:10.36 CS.seek 3.000ml @09:05:18.07 delay 5 seconds @09:05:23.09 CSV.dial :air @09:05:23.56 CS.seek 4.999ml @09:05:29.04 CSV.dial :air @09:05:29.05 CS.seek 0.000ml @09:05:41.54 delay 7 seconds @09:05:48.56 CS.seek 3.000ml @09:05:56.44 CSV.dial :waste @09:05:57.23 CS.seek 0.000ml @09:06:05.03 Garaging FlushPuck in Collection Clamp @09:06:05.08 Elbow.reconfigure TightElbow @09:06:05.12 Elbow.seek CC @09:06:05.34 Elbow.reconfigure LooseElbow @09:06:05.43 Forearm.seek CC @09:06:06.38 CC.seek sealed @09:06:07.32 Hand.grip @09:06:08.12 CC.seek open @09:06:11.41 Forearm.seek retract @09:06:12.34 Elbow.reconfigure TightElbow @09:06:12.38 Elbow.seek garage @09:06:15.76 Elbow.reconfigure LooseElbow @09:06:15.85 Forearm.reconfigure TightForearm @09:06:15.88 Forearm.seek garage @09:06:17.06 Hand.release @09:06:17.78 Forearm.reconfigure LooseForearm @09:06:17.81 Forearm.seek retract @09:06:18.91 Starting mfb @09:06:18.92 pcrBAC sampling at most 1000ml, wcr at most 1000ml @09:06:18.94 Loading sh1 puck from tube 2 into Collection Clamp @09:06:18.97 BACfans.email "Can@09:02:05, 16.8C, 63% humidity, 14.3psia, 13.709V, 0.652A, 0.659A avg, 8.95W\nCTD data unavailable\nISUS data unavailable",Subject => "pcrBAC sampling at most 1000ml, wcr at most 1000ml" @09:06:20.69 Readying the MFB @09:06:20.70 Homing MFB Syringe @09:06:20.71 AFV.select :holdingCoil @09:06:20.97 ATV.dial :air2 @09:06:21.01
SC.reconfigure SCloose @09:06:21.21 Elbow.reconfigure TightElbow @09:06:21.24 Elbow.seek 2 @09:06:22.26 AS.reconfigure ASconfig @09:06:22.29 AS.home.seek home @09:06:23.07
Forearm.reconfigure TightForearm @09:06:23.10 Forearm.seek 2 + 800 counts @09:06:24.09 SE.reconfigure SEpick @09:06:24.15 SE.seek beamBreak @09:06:26.51 SE.configure SEconfig @09:06:26.65 Elbow.seek 2 @09:06:26.80 Forearm.seek 2 + 300 counts @09:06:27.04 Forearm.reconfigure LooseForearm @09:06:27.08 Hand.grip @09:06:27.87 SE.seek down @09:06:28.03
Forearm.seek retract @09:06:29.05 Elbow.seek CC @09:06:29.78 AFV.select :carrierSolution @09:06:29.88 delay 2 seconds @09:06:30.13 SE.coast @09:06:30.56
Elbow.reconfigure LooseElbow @09:06:30.64 Forearm.reconfigure TightForearm @09:06:30.67 Forearm.seek CC @09:06:31.78 CC.seek closed @09:06:31.89 AS.seek 1.000ml @09:06:35.98
Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.93 @09:06:36.00 Hand.release @09:06:36.78 Forearm.reconfigure LooseForearm @09:06:36.81 Forearm.seek retract @09:06:37.79 SC.coast @09:06:37.82 collectSample 1000ml w/bubble point of 55psi Priming 7ml @09:06:37.83 Sleepy.power :sampler,:ON @09:06:37.84 Intake.select :close @09:06:37.85 Exhaust.select :close SS.hold @09:06:38.02 Calibrating pressure and temperature sensors @09:06:38.03 CTV.dial :air @09:06:38.04 SSV.dial :spit,avoiding => exhaust @09:06:38.06 SS.reconfigure SSfast @09:06:38.09 SS.seek 5.00ml @09:06:41.22 SSV.dial CBV @09:06:41.24
CBV.dial SSV @09:06:42.63 delay 3.8 seconds @09:06:46.51 SSV.dial :spit @09:06:47.88 SS.seek 0.00ml @09:06:50.34 SSV.dial CBV @09:06:51.69 CTV.dial :intake @09:06:52.44 Waiting for external sampler to finish priming... @09:06:52.45 delayUntil "09:16:37.83" @09:07:23.62 delay 1 second @09:07:24.63 AFV.select :holdingCoil @09:07:24.72 delay 1 second @09:07:26.27 ATV.dial :waste @09:07:27.18 AS.reconfigure ASnorm @09:07:27.21 AS.home.seek empty @09:08:19.23 ATV.dial :waste @09:08:19.24 ARV.dial between carrier and air1 @09:08:19.31 ASV.dial :waste1 @09:16:37.84 SSV.dial CBV @09:16:37.86
CBV.dial SSV @09:16:37.87 Allowing 55s for 7.0ml of remaining 7.0ml Intake.select :open @09:16:40.00 Intake @39.7psia, vacuum from 20.0 to 24.0psi @09:16:40.01 SS.reconfigure forSampling @09:16:40.05 SS.seek 7.00ml @09:16:45.70 Stroke completed -- waiting for pressure to equalize ... @09:16:46.34 Intake.select :close @09:16:46.48 SSV.dial :exhaust @09:16:47.27 Exhaust.select :open @09:16:47.36 SS.reconfigure SSexhale @09:16:47.40 SS.seek 0.00ml @09:16:51.31 Exhaust.select :close @09:16:51.41 Sampling 1000.0ml @09:16:51.42 Intake.select :close @09:16:51.43 Exhaust.select :close @09:16:51.44 SS.reconfigure SSconfig @09:16:51.47 SS.hold @09:16:51.62 CTV.dial :intake @09:16:51.63 SSV.dial CBV @09:16:51.65
CBV.dial SSV @09:16:52.46 Allowing 185s for 25.0ml of remaining 1000.0ml Intake.select :open @09:16:54.59 Intake @39.7psia, vacuum from 20.0 to 24.0psi @09:16:54.60 SS.reconfigure forSampling @09:16:54.64 SS.seek 25.00ml @09:17:09.40 Stroke completed -- waiting for pressure to equalize ... @09:17:10.04 Intake.select :close @09:17:10.17 SSV.dial :exhaust @09:17:10.98 Exhaust.select :open @09:17:11.08 SS.reconfigure SSexhale @09:17:11.12 SS.seek 0.00ml @09:17:19.69 Exhaust.select :close @09:17:19.78 SSV.dial CBV @09:17:19.80
CBV.dial SSV @09:17:20.62 Allowing 185s for 25.0ml of remaining 975.0ml @09:17:20.63 Intake.select :open @09:17:22.78 Intake @40.4psia, vacuum from 20.0 to 24.0psi @09:17:22.79 SS.reconfigure forSampling @09:17:22.83 SS.seek 25.00ml @09:17:35.36 Vacuum too high -- waiting up to 40s @09:17:35.63 Intake @32.6psia, vacuum from 20.0 to 24.0psi @09:17:35.64 SS.reconfigure forSampling @09:17:35.67 SS.seek 25.00ml @09:17:37.71 Stroke completed -- waiting for pressure to equalize ... @09:17:38.35 Intake.select :close @09:17:38.48 SSV.dial :exhaust @09:17:39.29 Exhaust.select :open @09:17:39.38 SS.reconfigure SSexhale @09:17:39.42 SS.seek 0.00ml @09:17:48.13 Exhaust.select :close @09:17:48.22 SSV.dial CBV @09:17:48.24
CBV.dial SSV @09:17:49.05 Allowing 185s for 25.0ml of remaining 950.0ml @09:17:49.06 Intake.select :open @09:17:51.19 Intake @40.8psia, vacuum from 20.0 to 24.0psi @09:17:51.20 SS.reconfigure forSampling @09:17:51.24 SS.seek 25.00ml @09:17:57.92 Vacuum too high -- waiting up to 40s @09:17:58.18 Intake @32.8psia, vacuum from 20.0 to 24.0psi @09:17:58.19 SS.reconfigure forSampling @09:17:58.23 SS.seek 25.00ml @09:18:07.83 Stroke completed -- waiting for pressure to equalize ... @09:18:08.46 Intake.select :close @09:18:08.60 SSV.dial :exhaust @09:18:09.41 Exhaust.select :open @09:18:09.50 SS.reconfigure SSexhale @09:18:09.54 SS.seek 0.00ml @09:18:18.57 Exhaust.select :close @09:18:18.66 SSV.dial CBV @09:18:18.68
CBV.dial SSV @09:18:19.47 Allowing 185s for 25.0ml of remaining 925.0ml @09:18:19.48 Intake.select :open @09:18:21.61 Intake @40.4psia, vacuum from 20.0 to 24.0psi @09:18:21.62 SS.reconfigure forSampling @09:18:21.66 SS.seek 25.00ml @09:18:25.02 Vacuum too high -- waiting up to 40s @09:18:25.28 Intake @34.3psia, vacuum from 20.0 to 24.0psi @09:18:25.29 SS.reconfigure forSampling @09:18:25.32 SS.seek 25.00ml @09:18:40.91 Stroke completed -- waiting for pressure to equalize ... @09:18:41.54 Intake.select :close @09:18:41.68 SSV.dial :exhaust @09:18:42.49 Exhaust.select :open @09:18:42.60 SS.reconfigure SSexhale @09:18:42.70 SS.seek 0.00ml @09:18:51.39 Exhaust.select :close @09:18:51.48 SSV.dial CBV @09:18:51.50
CBV.dial SSV @09:18:52.31 Allowing 185s for 25.0ml of remaining 900.0ml @09:18:52.32 Intake.select :open @09:18:54.45 Intake @40.7psia, vacuum from 20.0 to 24.0psi @09:18:54.46 SS.reconfigure forSampling @09:18:54.50 SS.seek 25.00ml @09:18:57.18 Vacuum too high -- waiting up to 40s @09:18:57.44 Intake @36.1psia, vacuum from 20.0 to 24.0psi @09:18:57.45 SS.reconfigure forSampling @09:18:57.48 SS.seek 25.00ml @09:19:01.95 Vacuum too high -- waiting up to 40s @09:19:02.21 Intake @36.0psia, vacuum from 20.0 to 24.0psi @09:19:02.22 SS.reconfigure forSampling @09:19:02.26 SS.seek 25.00ml @09:19:13.17 Vacuum too high -- waiting up to 40s @09:19:13.64 Intake @38.9psia, vacuum from 20.0 to 24.0psi @09:19:13.65 SS.reconfigure forSampling @09:19:14.23 SS.seek 25.00ml @09:19:22.51 Vacuum too high -- waiting up to 40s @09:19:22.78 Intake @38.7psia, vacuum from 20.0 to 24.0psi @09:19:22.79 SS.reconfigure forSampling @09:19:22.82 SS.seek 25.00ml @09:19:23.50 Stroke completed -- waiting for pressure to equalize ... @09:19:24.14 Intake.select :close @09:19:24.29 SSV.dial :exhaust @09:19:25.09 Exhaust.select :open @09:19:25.20 SS.reconfigure SSexhale @09:19:25.24 SS.seek 0.00ml @09:19:34.48 Exhaust.select :close @09:19:34.57 SSV.dial CBV @09:19:34.59
CBV.dial SSV @09:19:35.39 Allowing 185s for 25.0ml of remaining 875.0ml @09:19:35.40 Intake.select :open @09:19:37.55 Intake @40.5psia, vacuum from 20.0 to 24.0psi @09:19:37.56 SS.reconfigure forSampling @09:19:37.60 SS.seek 25.00ml @09:20:01.07 Vacuum too high -- waiting up to 40s @09:20:01.34 Intake @39.8psia, vacuum from 20.0 to 24.0psi @09:20:01.35 SS.reconfigure forSampling @09:20:01.38 SS.seek 25.00ml @09:20:25.68 Stroke completed -- waiting for pressure to equalize ... @09:20:26.32 Intake.select :close @09:20:26.45 SSV.dial :exhaust @09:20:27.25 Exhaust.select :open @09:20:27.34 SS.reconfigure SSexhale @09:20:27.38 SS.seek 0.00ml @09:20:36.06 Exhaust.select :close @09:20:36.15 SSV.dial CBV @09:20:36.17
CBV.dial SSV @09:20:36.98 Allowing 185s for 25.0ml of remaining 850.0ml @09:20:36.99 Intake.select :open @09:20:39.12 Intake @41.0psia, vacuum from 20.0 to 24.0psi @09:20:39.13 SS.reconfigure forSampling @09:20:39.17 SS.seek 25.00ml @09:21:21.91 Vacuum too high -- waiting up to 40s @09:21:22.18 Intake @40.3psia, vacuum from 20.0 to 24.0psi @09:21:22.19 SS.reconfigure forSampling @09:21:22.22 SS.seek 25.00ml @09:21:52.98 Stroke completed -- waiting for pressure to equalize ... @09:21:53.62 Intake.select :close @09:21:53.75 SSV.dial :exhaust @09:21:54.57 Exhaust.select :open @09:21:54.68 SS.reconfigure SSexhale @09:21:54.72 SS.seek 0.00ml @09:22:03.40 Exhaust.select :close @09:22:03.49 SSV.dial CBV @09:22:03.51
CBV.dial SSV @09:22:04.32 Allowing 185s for 25.0ml of remaining 825.0ml @09:22:04.33 Intake.select :open @09:22:06.46 Intake @39.5psia, vacuum from 20.0 to 24.0psi @09:22:06.47 SS.reconfigure forSampling @09:22:06.51 SS.seek 25.00ml @09:23:56.86 Stroke completed -- waiting for pressure to equalize ... @09:23:58.07 Intake.select :close @09:23:58.22 SSV.dial :exhaust @09:23:59.02 Exhaust.select :open @09:23:59.11 SS.reconfigure SSexhale @09:23:59.15 SS.seek 0.00ml @09:24:08.03 Exhaust.select :close @09:24:08.12 SSV.dial CBV @09:24:08.14
CBV.dial SSV @09:24:08.94 Allowing 185s for 25.0ml of remaining 800.0ml @09:24:08.95 Intake.select :open @09:24:11.08 Intake @39.0psia, vacuum from 20.0 to 24.0psi @09:24:11.09 SS.reconfigure forSampling @09:24:11.13 SS.seek 25.00ml @09:26:01.46 Stroke completed -- waiting for pressure to equalize ... @09:26:02.68 Intake.select :close @09:26:02.81 SSV.dial :exhaust @09:26:03.60 Exhaust.select :open @09:26:03.69 SS.reconfigure SSexhale @09:26:03.73 SS.seek 0.00ml @09:26:12.60 Exhaust.select :close @09:26:12.69 SSV.dial CBV @09:26:12.71
CBV.dial SSV @09:26:13.53 Allowing 185s for 25.0ml of remaining 775.0ml @09:26:13.54 Intake.select :open @09:26:15.67 Intake @42.6psia, vacuum from 20.0 to 24.0psi @09:26:15.68 SS.reconfigure forSampling @09:26:15.72 SS.seek 25.00ml @09:27:06.18 Vacuum too high -- waiting up to 40s @09:27:06.64 Intake @42.5psia, vacuum from 20.0 to 24.0psi @09:27:06.65 SS.reconfigure forSampling @09:27:06.69 SS.seek 25.00ml @09:27:15.08 Vacuum too high -- waiting up to 40s @09:27:15.57 Intake @42.4psia, vacuum from 20.0 to 24.0psi @09:27:15.58 SS.reconfigure forSampling @09:27:15.61 SS.seek 25.00ml @09:27:23.43 Vacuum too high -- waiting up to 40s @09:27:23.89 Intake @42.6psia, vacuum from 20.0 to 24.0psi @09:27:23.90 SS.reconfigure forSampling @09:27:23.94 SS.seek 25.00ml @09:27:27.30 Vacuum too high -- waiting up to 40s @09:27:27.78 Intake @42.4psia, vacuum from 20.0 to 24.0psi @09:27:27.79 SS.reconfigure forSampling @09:27:27.82 SS.seek 25.00ml @09:27:33.42 Vacuum too high -- waiting up to 40s @09:27:34.45 SS.seek 25.00ml @09:27:38.55 Vacuum too high -- waiting up to 40s @09:27:39.03 Intake @42.3psia, vacuum from 20.0 to 24.0psi @09:27:39.04 SS.reconfigure forSampling @09:27:39.08 SS.seek 25.00ml @09:27:42.74 Vacuum too high -- waiting up to 40s @09:27:43.22 Intake @42.9psia, vacuum from 20.0 to 24.0psi @09:27:43.23 SS.reconfigure forSampling @09:27:43.26 SS.seek 25.00ml @09:27:46.71 Vacuum too high -- waiting up to 40s @09:27:47.18 Intake @42.5psia, vacuum from 20.0 to 24.0psi @09:27:47.19 SS.reconfigure forSampling @09:27:47.22 SS.seek 25.00ml @09:27:50.06 Vacuum too high -- waiting up to 40s @09:27:50.54 Intake @42.7psia, vacuum from 20.0 to 24.0psi @09:27:50.55 SS.reconfigure forSampling @09:27:50.59 SS.seek 25.00ml @09:27:53.28 Vacuum too high -- waiting up to 40s @09:27:53.76 Intake @42.5psia, vacuum from 20.0 to 24.0psi @09:27:53.77 SS.reconfigure forSampling @09:27:53.80 SS.seek 25.00ml @09:27:56.71 Vacuum too high -- waiting up to 40s @09:27:57.19 Intake @43.1psia, vacuum from 20.0 to 24.0psi @09:27:57.20 SS.reconfigure forSampling @09:27:57.23 SS.seek 25.00ml @09:27:59.93 Vacuum too high -- waiting up to 40s @09:28:00.41 Intake @43.2psia, vacuum from 20.0 to 24.0psi @09:28:00.42 SS.reconfigure forSampling @09:28:00.45 SS.seek 25.00ml @09:28:02.93 Vacuum too high -- waiting up to 40s @09:28:03.41 Intake @42.9psia, vacuum from 20.0 to 24.0psi @09:28:03.42 SS.reconfigure forSampling @09:28:03.45 SS.seek 25.00ml @09:28:05.71 Vacuum too high -- waiting up to 40s @09:28:06.19 Intake @42.7psia, vacuum from 20.0 to 24.0psi @09:28:06.20 SS.reconfigure forSampling @09:28:06.23 SS.seek 25.00ml @09:28:08.96 Vacuum too high -- waiting up to 40s @09:28:09.43 Intake @44.0psia, vacuum from 20.0 to 24.0psi @09:28:09.44 SS.reconfigure forSampling @09:28:09.48 SS.seek 25.00ml @09:28:11.74 Vacuum too high -- waiting up to 40s @09:28:12.21 Intake @43.4psia, vacuum from 20.0 to 24.0psi @09:28:12.22 SS.reconfigure forSampling @09:28:12.25 SS.seek 25.00ml @09:28:14.40 Vacuum too high -- waiting up to 40s @09:28:14.88 Intake @43.2psia, vacuum from 20.0 to 24.0psi @09:28:14.89 SS.reconfigure forSampling @09:28:14.92 SS.seek 25.00ml @09:28:17.21 Vacuum too high -- waiting up to 40s @09:28:17.68 Intake @43.0psia, vacuum from 20.0 to 24.0psi @09:28:17.69 SS.reconfigure forSampling @09:28:17.72 SS.seek 25.00ml @09:28:20.34 Vacuum too high -- waiting up to 40s @09:28:20.81 Intake @44.0psia, vacuum from 20.0 to 24.0psi @09:28:20.82 SS.reconfigure forSampling @09:28:20.86 SS.seek 25.00ml @09:28:21.39 Stroke completed -- waiting for pressure to equalize ... @09:28:22.61 Intake.select :close @09:28:22.74 SSV.dial :exhaust @09:28:23.55 Exhaust.select :open @09:28:23.66 SS.reconfigure SSexhale @09:28:23.70 SS.seek 0.00ml @09:28:32.66 Exhaust.select :close @09:28:32.75 SSV.dial CBV @09:28:32.77
CBV.dial SSV @09:28:33.57 Allowing 185s for 25.0ml of remaining 750.0ml @09:28:33.58 Intake.select :open @09:28:35.71 Intake @42.9psia, vacuum from 20.0 to 24.0psi @09:28:35.72 SS.reconfigure forSampling @09:28:35.76 SS.seek 25.00ml @09:28:39.23 Vacuum too high -- waiting up to 40s @09:28:39.70 Intake @43.3psia, vacuum from 20.0 to 24.0psi @09:28:39.71 SS.reconfigure forSampling @09:28:39.75 SS.seek 25.00ml @09:28:42.68 Vacuum too high -- waiting up to 40s @09:28:43.15 Intake @43.6psia, vacuum from 20.0 to 24.0psi @09:28:43.16 SS.reconfigure forSampling @09:28:43.20 SS.seek 25.00ml @09:28:44.93 Vacuum too high -- waiting up to 40s @09:28:45.18 Intake @42.8psia, vacuum from 20.0 to 24.0psi @09:28:45.19 SS.reconfigure forSampling @09:28:45.23 SS.seek 25.00ml @09:28:47.40 Vacuum too high -- waiting up to 40s @09:28:47.67 Intake @43.4psia, vacuum from 20.0 to 24.0psi @09:28:47.68 SS.reconfigure forSampling @09:28:47.72 SS.seek 25.00ml @09:28:49.71 Vacuum too high -- waiting up to 40s @09:28:49.97 Intake @44.0psia, vacuum from 20.0 to 24.0psi @09:28:49.98 SS.reconfigure forSampling @09:28:50.02 SS.seek 25.00ml @09:28:51.37 Vacuum too high -- waiting up to 40s @09:28:51.63 Intake @43.7psia, vacuum from 20.0 to 24.0psi @09:28:51.64 SS.reconfigure forSampling @09:28:51.68 SS.seek 25.00ml @09:28:52.99 Vacuum too high -- waiting up to 40s @09:28:53.25 Intake @43.8psia, vacuum from 20.0 to 24.0psi @09:28:53.26 SS.reconfigure forSampling @09:28:53.30 SS.seek 25.00ml @09:28:54.58 Vacuum too high -- waiting up to 40s @09:28:54.85 Intake @43.6psia, vacuum from 20.0 to 24.0psi @09:28:54.86 SS.reconfigure forSampling @09:28:54.89 SS.seek 25.00ml @09:28:56.34 Vacuum too high -- waiting up to 40s @09:28:56.60 Intake @43.5psia, vacuum from 20.0 to 24.0psi @09:28:56.61 SS.reconfigure forSampling @09:28:56.64 SS.seek 25.00ml @09:28:57.99 Vacuum too high -- waiting up to 40s @09:28:58.25 Intake @43.3psia, vacuum from 20.0 to 24.0psi @09:28:58.26 SS.reconfigure forSampling @09:28:58.29 SS.seek 25.00ml @09:28:59.80 Vacuum too high -- waiting up to 40s @09:29:00.06 Intake @43.1psia, vacuum from 20.0 to 24.0psi @09:29:00.07 SS.reconfigure forSampling @09:29:00.10 SS.seek 25.00ml @09:29:01.49 Vacuum too high -- waiting up to 40s @09:29:01.75 Intake @43.5psia, vacuum from 20.0 to 24.0psi @09:29:01.76 SS.reconfigure forSampling @09:29:01.79 SS.seek 25.00ml @09:29:03.33 Vacuum too high -- waiting up to 40s @09:29:03.59 Intake @44.1psia, vacuum from 20.0 to 24.0psi @09:29:03.60 SS.reconfigure forSampling @09:29:03.64 SS.seek 25.00ml @09:29:04.77 Vacuum too high -- waiting up to 40s @09:29:05.03 Intake @43.9psia, vacuum from 20.0 to 24.0psi @09:29:05.04 SS.reconfigure forSampling @09:29:05.07 SS.seek 25.00ml @09:29:06.37 Vacuum too high -- waiting up to 40s @09:29:06.63 Intake @43.6psia, vacuum from 20.0 to 24.0psi @09:29:06.64 SS.reconfigure forSampling @09:29:06.67 SS.seek 25.00ml @09:29:07.90 Vacuum too high -- waiting up to 40s @09:29:08.16 Intake @43.4psia, vacuum from 20.0 to 24.0psi @09:29:08.17 SS.reconfigure forSampling @09:29:08.20 SS.seek 25.00ml @09:29:09.68 Vacuum too high -- waiting up to 40s @09:29:10.15 Intake @43.3psia, vacuum from 20.0 to 24.0psi @09:29:10.16 SS.reconfigure forSampling @09:29:10.19 SS.seek 25.00ml @09:29:12.21 Vacuum too high -- waiting up to 40s @09:29:12.67 Intake @43.4psia, vacuum from 20.0 to 24.0psi @09:29:12.68 SS.reconfigure forSampling @09:29:12.72 SS.seek 25.00ml @09:29:14.90 Vacuum too high -- waiting up to 40s @09:29:15.37 Intake @44.3psia, vacuum from 20.0 to 24.0psi @09:29:15.38 SS.reconfigure forSampling @09:29:15.41 SS.seek 25.00ml @09:29:16.68 Vacuum too high -- waiting up to 40s @09:29:16.94 Intake @43.8psia, vacuum from 20.0 to 24.0psi @09:29:16.95 SS.reconfigure forSampling @09:29:16.98 SS.seek 25.00ml @09:29:18.27 Vacuum too high -- waiting up to 40s @09:29:18.54 SS.seek 25.00ml @09:29:19.80 Vacuum too high -- waiting up to 40s @09:29:20.27 Intake @43.7psia, vacuum from 20.0 to 24.0psi @09:29:20.28 SS.reconfigure forSampling @09:29:20.31 SS.seek 25.00ml @09:29:21.68 Vacuum too high -- waiting up to 40s @09:29:21.93 Intake @43.6psia, vacuum from 20.0 to 24.0psi @09:29:21.94 SS.reconfigure forSampling @09:29:21.98 SS.seek 25.00ml @09:29:23.74 Vacuum too high -- waiting up to 40s @09:29:24.21 Intake @43.6psia, vacuum from 20.0 to 24.0psi @09:29:24.22 SS.reconfigure forSampling @09:29:24.25 SS.seek 25.00ml @09:29:26.02 Vacuum too high -- waiting up to 40s @09:29:26.48 Intake @44.1psia, vacuum from 20.0 to 24.0psi @09:29:26.49 SS.reconfigure forSampling @09:29:26.53 SS.seek 25.00ml @09:29:27.83 Vacuum too high -- waiting up to 40s @09:29:28.08 Intake @43.9psia, vacuum from 20.0 to 24.0psi @09:29:28.09 SS.reconfigure forSampling @09:29:28.13 SS.seek 25.00ml @09:29:29.86 Vacuum too high -- waiting up to 40s @09:29:30.12 Intake @43.5psia, vacuum from 20.0 to 24.0psi @09:29:30.13 SS.reconfigure forSampling @09:29:30.16 SS.seek 25.00ml @09:29:32.36 Vacuum too high -- waiting up to 40s @09:29:32.83 Intake @43.6psia, vacuum from 20.0 to 24.0psi @09:29:32.84 SS.reconfigure forSampling @09:29:32.87 SS.seek 25.00ml @09:29:34.61 Vacuum too high -- waiting up to 40s @09:29:35.08 Intake @44.0psia, vacuum from 20.0 to 24.0psi @09:29:35.09 SS.reconfigure forSampling @09:29:35.12 SS.seek 25.00ml @09:29:36.33 Vacuum too high -- waiting up to 40s @09:29:36.80 Intake @44.3psia, vacuum from 20.0 to 24.0psi @09:29:36.81 SS.reconfigure forSampling @09:29:36.85 SS.seek 25.00ml @09:29:38.11 Vacuum too high -- waiting up to 40s @09:29:38.37 Intake @43.5psia, vacuum from 20.0 to 24.0psi @09:29:38.38 SS.reconfigure forSampling @09:29:38.41 SS.seek 25.00ml @09:29:40.05 Vacuum too high -- waiting up to 40s @09:29:40.53 Intake @42.8psia, vacuum from 20.0 to 24.0psi @09:29:40.54 SS.reconfigure forSampling @09:29:40.58 SS.seek 25.00ml @09:29:42.55 Vacuum too high -- waiting up to 40s @09:29:43.01 Intake @43.0psia, vacuum from 20.0 to 24.0psi @09:29:43.03 SS.reconfigure forSampling @09:29:43.06 SS.seek 25.00ml @09:29:44.83 Vacuum too high -- waiting up to 40s @09:29:45.31 Intake @43.3psia, vacuum from 20.0 to 24.0psi @09:29:45.32 SS.reconfigure forSampling @09:29:45.36 SS.seek 25.00ml @09:29:46.99 Vacuum too high -- waiting up to 40s @09:29:47.47 Intake @42.9psia, vacuum from 20.0 to 24.0psi @09:29:47.48 SS.reconfigure forSampling @09:29:47.51 SS.seek 25.00ml @09:29:49.36 Vacuum too high -- waiting up to 40s @09:29:49.83 Intake @43.3psia, vacuum from 20.0 to 24.0psi @09:29:49.84 SS.reconfigure forSampling @09:29:49.87 SS.seek 25.00ml @09:29:51.61 Vacuum too high -- waiting up to 40s @09:29:52.09 Intake @43.3psia, vacuum from 20.0 to 24.0psi @09:29:52.10 SS.reconfigure forSampling @09:29:52.13 SS.seek 25.00ml @09:29:53.86 Vacuum too high -- waiting up to 40s @09:29:54.34 Intake @43.1psia, vacuum from 20.0 to 24.0psi @09:29:54.35 SS.reconfigure forSampling @09:29:54.38 SS.seek 25.00ml @09:29:56.08 Vacuum too high -- waiting up to 40s @09:29:56.56 Intake @42.8psia, vacuum from 20.0 to 24.0psi @09:29:56.57 SS.reconfigure forSampling @09:29:56.60 SS.seek 25.00ml @09:29:58.27 Vacuum too high -- waiting up to 40s @09:29:58.75 Intake @42.7psia, vacuum from 20.0 to 24.0psi @09:29:58.76 SS.reconfigure forSampling @09:29:58.79 SS.seek 25.00ml @09:30:00.46 Vacuum too high -- waiting up to 40s @09:30:00.94 Intake @43.0psia, vacuum from 20.0 to 24.0psi @09:30:00.95 SS.reconfigure forSampling @09:30:00.98 SS.seek 25.00ml @09:30:02.74 Vacuum too high -- waiting up to 40s @09:30:03.21 Intake @43.5psia, vacuum from 20.0 to 24.0psi @09:30:03.22 SS.reconfigure forSampling @09:30:03.25 SS.seek 25.00ml @09:30:04.80 Vacuum too high -- waiting up to 40s @09:30:05.28 Intake @42.9psia, vacuum from 20.0 to 24.0psi @09:30:05.29 SS.reconfigure forSampling @09:30:05.32 SS.seek 25.00ml @09:30:06.89 Vacuum too high -- waiting up to 40s @09:30:07.37 Intake @42.7psia, vacuum from 20.0 to 24.0psi @09:30:07.38 SS.reconfigure forSampling @09:30:07.41 SS.seek 25.00ml @09:30:09.14 Vacuum too high -- waiting up to 40s @09:30:09.62 Intake @42.8psia, vacuum from 20.0 to 24.0psi @09:30:09.63 SS.reconfigure forSampling @09:30:09.66 SS.seek 25.00ml @09:30:11.30 Vacuum too high -- waiting up to 40s @09:30:11.77 Intake @43.3psia, vacuum from 20.0 to 24.0psi @09:30:11.78 SS.reconfigure forSampling @09:30:11.81 SS.seek 25.00ml @09:30:14.49 Vacuum too high -- waiting up to 40s @09:30:14.97 Intake @43.3psia, vacuum from 20.0 to 24.0psi @09:30:14.98 SS.reconfigure forSampling @09:30:15.01 SS.seek 25.00ml @09:30:16.58 Vacuum too high -- waiting up to 40s @09:30:17.06 Intake @42.9psia, vacuum from 20.0 to 24.0psi @09:30:17.07 SS.reconfigure forSampling @09:30:17.10 SS.seek 25.00ml @09:30:18.74 Vacuum too high -- waiting up to 40s @09:30:19.21 Intake @42.9psia, vacuum from 20.0 to 24.0psi @09:30:19.22 SS.reconfigure forSampling @09:30:19.26 SS.seek 25.00ml @09:30:21.03 Vacuum too high -- waiting up to 40s @09:30:21.50 Intake @43.3psia, vacuum from 20.0 to 24.0psi @09:30:21.51 SS.reconfigure forSampling @09:30:21.55 SS.seek 25.00ml @09:30:23.39 Vacuum too high -- waiting up to 40s @09:30:23.87 Intake @43.5psia, vacuum from 20.0 to 24.0psi @09:30:23.88 SS.reconfigure forSampling @09:30:23.92 SS.seek 25.00ml @09:30:25.36 Vacuum too high -- waiting up to 40s @09:30:25.85 Intake @42.9psia, vacuum from 20.0 to 24.0psi @09:30:25.86 SS.reconfigure forSampling @09:30:25.89 SS.seek 25.00ml @09:30:27.46 Vacuum too high -- waiting up to 40s @09:30:27.94 Intake @43.1psia, vacuum from 20.0 to 24.0psi @09:30:27.95 SS.reconfigure forSampling @09:30:27.98 SS.seek 25.00ml @09:30:29.58 Vacuum too high -- waiting up to 40s @09:30:30.05 Intake @43.5psia, vacuum from 20.0 to 24.0psi @09:30:30.06 SS.reconfigure forSampling @09:30:30.09 SS.seek 25.00ml @09:30:31.49 Vacuum too high -- waiting up to 40s @09:30:31.96 Intake @43.3psia, vacuum from 20.0 to 24.0psi @09:30:31.97 SS.reconfigure forSampling @09:30:32.00 SS.seek 25.00ml @09:30:33.55 Vacuum too high -- waiting up to 40s @09:30:34.02 Intake @43.4psia, vacuum from 20.0 to 24.0psi @09:30:34.03 SS.reconfigure forSampling @09:30:34.07 SS.seek 25.00ml @09:30:35.58 Vacuum too high -- waiting up to 40s @09:30:36.06 Intake @43.2psia, vacuum from 20.0 to 24.0psi @09:30:36.07 SS.reconfigure forSampling @09:30:36.10 SS.seek 25.00ml @09:30:37.67 Vacuum too high -- waiting up to 40s @09:30:38.15 Intake @43.6psia, vacuum from 20.0 to 24.0psi @09:30:38.16 SS.reconfigure forSampling @09:30:38.19 SS.seek 25.00ml @09:30:39.77 Vacuum too high -- waiting up to 40s @09:30:40.25 Intake @43.3psia, vacuum from 20.0 to 24.0psi @09:30:40.26 SS.reconfigure forSampling @09:30:40.30 SS.seek 25.00ml @09:30:41.80 Vacuum too high -- waiting up to 40s @09:30:42.27 Intake @43.0psia, vacuum from 20.0 to 24.0psi @09:30:42.28 SS.reconfigure forSampling @09:30:42.31 SS.seek 25.00ml @09:30:43.80 Vacuum too high -- waiting up to 40s @09:30:44.27 Intake @43.5psia, vacuum from 20.0 to 24.0psi @09:30:44.28 SS.reconfigure forSampling @09:30:44.31 SS.seek 25.00ml @09:30:45.86 Vacuum too high -- waiting up to 40s @09:30:46.33 Intake @43.3psia, vacuum from 20.0 to 24.0psi @09:30:46.34 SS.reconfigure forSampling @09:30:46.37 SS.seek 25.00ml @09:30:47.80 Vacuum too high -- waiting up to 40s @09:30:48.27 Intake @43.5psia, vacuum from 20.0 to 24.0psi @09:30:48.28 SS.reconfigure forSampling @09:30:48.31 SS.seek 25.00ml @09:30:49.67 Vacuum too high -- waiting up to 40s @09:30:50.14 Intake @43.6psia, vacuum from 20.0 to 24.0psi @09:30:50.15 SS.reconfigure forSampling @09:30:50.18 SS.seek 25.00ml @09:30:51.64 Vacuum too high -- waiting up to 40s @09:30:52.11 Intake @43.0psia, vacuum from 20.0 to 24.0psi @09:30:52.12 SS.reconfigure forSampling @09:30:52.15 SS.seek 25.00ml @09:30:53.55 Vacuum too high -- waiting up to 40s @09:30:54.62 Intake @43.1psia, vacuum from 20.0 to 24.0psi @09:30:54.63 SS.reconfigure forSampling @09:30:54.67 SS.seek 25.00ml @09:30:56.61 Vacuum too high -- waiting up to 40s @09:30:57.08 Intake @43.2psia, vacuum from 20.0 to 24.0psi @09:30:57.09 SS.reconfigure forSampling @09:30:57.12 SS.seek 25.00ml @09:30:58.70 Vacuum too high -- waiting up to 40s @09:30:59.18 Intake @44.0psia, vacuum from 20.0 to 24.0psi @09:30:59.19 SS.reconfigure forSampling @09:30:59.23 SS.seek 25.00ml @09:31:00.58 Vacuum too high -- waiting up to 40s @09:31:01.05 Intake @43.6psia, vacuum from 20.0 to 24.0psi @09:31:01.06 SS.reconfigure forSampling @09:31:01.09 SS.seek 25.00ml @09:31:02.61 Vacuum too high -- waiting up to 40s @09:31:03.09 Intake @43.5psia, vacuum from 20.0 to 24.0psi @09:31:03.10 SS.reconfigure forSampling @09:31:03.13 SS.seek 25.00ml @09:31:04.42 Vacuum too high -- waiting up to 40s @09:31:04.89 Intake @43.1psia, vacuum from 20.0 to 24.0psi @09:31:04.90 SS.reconfigure forSampling @09:31:04.94 SS.seek 25.00ml @09:31:06.36 Vacuum too high -- waiting up to 40s @09:31:06.83 Intake @43.1psia, vacuum from 20.0 to 24.0psi @09:31:06.84 SS.reconfigure forSampling @09:31:06.88 SS.seek 25.00ml @09:31:08.30 Vacuum too high -- waiting up to 40s @09:31:08.78 Intake @42.9psia, vacuum from 20.0 to 24.0psi @09:31:08.79 SS.reconfigure forSampling @09:31:08.82 SS.seek 25.00ml @09:31:10.30 Vacuum too high -- waiting up to 40s @09:31:10.78 Intake @43.1psia, vacuum from 20.0 to 24.0psi @09:31:10.81 SS.reconfigure forSampling @09:31:10.84 SS.seek 25.00ml @09:31:12.27 Vacuum too high -- waiting up to 40s @09:31:12.74 Intake @43.7psia, vacuum from 20.0 to 24.0psi @09:31:12.75 SS.reconfigure forSampling @09:31:12.78 SS.seek 25.00ml @09:31:14.20 Vacuum too high -- waiting up to 40s @09:31:14.66 Intake @43.8psia, vacuum from 20.0 to 24.0psi @09:31:14.67 SS.reconfigure forSampling @09:31:14.71 SS.seek 25.00ml @09:31:16.14 Vacuum too high -- waiting up to 40s @09:31:16.60 Intake @43.1psia, vacuum from 20.0 to 24.0psi @09:31:16.61 SS.reconfigure forSampling @09:31:16.65 SS.seek 25.00ml @09:31:18.02 Vacuum too high -- waiting up to 40s @09:31:18.49 Intake @43.4psia, vacuum from 20.0 to 24.0psi @09:31:18.50 SS.reconfigure forSampling @09:31:18.53 SS.seek 25.00ml @09:31:19.95 Vacuum too high -- waiting up to 40s @09:31:20.43 Intake @43.5psia, vacuum from 20.0 to 24.0psi @09:31:20.44 SS.reconfigure forSampling @09:31:20.48 SS.seek 25.00ml @09:31:20.76 Stroke completed -- waiting for pressure to equalize ... @09:31:22.56 Intake.select :close @09:31:22.71 SSV.dial :exhaust @09:31:23.51 Exhaust.select :open @09:31:23.60 SS.reconfigure SSexhale @09:31:23.64 SS.seek 0.00ml @09:31:32.43 Exhaust.select :close @09:31:32.52 SSV.dial CBV @09:31:32.54
CBV.dial SSV @09:31:33.35 Allowing 185s for 25.0ml of remaining 725.0ml @09:31:33.36 Intake.select :open @09:31:35.49 Intake @43.4psia, vacuum from 20.0 to 24.0psi @09:31:35.50 SS.reconfigure forSampling @09:31:35.54 SS.seek 25.00ml @09:31:37.80 Vacuum too high -- waiting up to 40s @09:31:38.27 Intake @43.9psia, vacuum from 20.0 to 24.0psi @09:31:38.28 SS.reconfigure forSampling @09:31:38.31 SS.seek 25.00ml @09:31:39.39 Vacuum too high -- waiting up to 40s @09:31:39.86 Intake @44.4psia, vacuum from 20.0 to 24.0psi @09:31:39.87 SS.reconfigure forSampling @09:31:39.90 SS.seek 25.00ml @09:31:41.03 Vacuum too high -- waiting up to 40s @09:31:41.30 Intake @44.0psia, vacuum from 20.0 to 24.0psi @09:31:41.31 SS.reconfigure forSampling @09:31:41.34 SS.seek 25.00ml @09:31:42.51 Vacuum too high -- waiting up to 40s @09:31:43.38 Intake @43.4psia, vacuum from 20.0 to 24.0psi @09:31:43.39 SS.reconfigure forSampling @09:31:43.42 SS.seek 25.00ml @09:31:45.23 Vacuum too high -- waiting up to 40s @09:31:45.71 Intake @43.2psia, vacuum from 20.0 to 24.0psi @09:31:45.72 SS.reconfigure forSampling @09:31:45.75 SS.seek 25.00ml @09:31:47.14 Vacuum too high -- waiting up to 40s @09:31:47.61 Intake @43.3psia, vacuum from 20.0 to 24.0psi @09:31:47.62 SS.reconfigure forSampling @09:31:47.65 SS.seek 25.00ml @09:31:48.98 Vacuum too high -- waiting up to 40s @09:31:49.45 Intake @44.3psia, vacuum from 20.0 to 24.0psi @09:31:49.46 SS.reconfigure forSampling @09:31:49.49 SS.seek 25.00ml @09:31:50.36 Vacuum too high -- waiting up to 40s @09:31:50.62 Intake @44.0psia, vacuum from 20.0 to 24.0psi @09:31:50.63 SS.reconfigure forSampling @09:31:50.67 SS.seek 25.00ml @09:31:51.45 Vacuum too high -- waiting up to 40s @09:31:51.71 Intake @43.3psia, vacuum from 20.0 to 24.0psi @09:31:51.72 SS.reconfigure forSampling @09:31:51.76 SS.seek 25.00ml @09:31:52.64 Vacuum too high -- waiting up to 40s @09:31:53.11 Intake @43.1psia, vacuum from 20.0 to 24.0psi @09:31:53.12 SS.reconfigure forSampling @09:31:53.15 SS.seek 25.00ml @09:31:54.23 Vacuum too high -- waiting up to 40s @09:31:54.71 SS.seek 25.00ml @09:31:55.79 Vacuum too high -- waiting up to 40s @09:31:56.26 Intake @43.0psia, vacuum from 20.0 to 24.0psi @09:31:56.27 SS.reconfigure forSampling @09:31:56.30 SS.seek 25.00ml @09:31:57.36 Vacuum too high -- waiting up to 40s @09:31:57.83 Intake @43.0psia, vacuum from 20.0 to 24.0psi @09:31:57.84 SS.reconfigure forSampling @09:31:57.88 SS.seek 25.00ml @09:31:59.14 Vacuum too high -- waiting up to 40s @09:31:59.62 Intake @43.3psia, vacuum from 20.0 to 24.0psi @09:31:59.63 SS.reconfigure forSampling @09:31:59.66 SS.seek 25.00ml @09:32:00.79 Vacuum too high -- waiting up to 40s @09:32:01.26 Intake @43.6psia, vacuum from 20.0 to 24.0psi @09:32:01.27 SS.reconfigure forSampling @09:32:01.31 SS.seek 25.00ml @09:32:02.18 Vacuum too high -- waiting up to 40s @09:32:02.66 Intake @43.4psia, vacuum from 20.0 to 24.0psi @09:32:02.67 SS.reconfigure forSampling @09:32:02.71 SS.seek 25.00ml @09:32:03.87 Vacuum too high -- waiting up to 40s @09:32:04.34 Intake @43.3psia, vacuum from 20.0 to 24.0psi @09:32:04.35 SS.reconfigure forSampling @09:32:04.38 SS.seek 25.00ml @09:32:05.68 Vacuum too high -- waiting up to 40s @09:32:06.14 Intake @42.9psia, vacuum from 20.0 to 24.0psi @09:32:06.16 SS.reconfigure forSampling @09:32:06.19 SS.seek 25.00ml @09:32:07.46 Vacuum too high -- waiting up to 40s @09:32:07.94 Intake @43.1psia, vacuum from 20.0 to 24.0psi @09:32:07.95 SS.reconfigure forSampling @09:32:07.98 SS.seek 25.00ml @09:32:09.30 Vacuum too high -- waiting up to 40s @09:32:09.76 Intake @43.3psia, vacuum from 20.0 to 24.0psi @09:32:09.77 SS.reconfigure forSampling @09:32:09.81 SS.seek 25.00ml @09:32:11.01 Vacuum too high -- waiting up to 40s @09:32:11.48 Intake @43.4psia, vacuum from 20.0 to 24.0psi @09:32:11.49 SS.reconfigure forSampling @09:32:11.53 SS.seek 25.00ml @09:32:12.77 Vacuum too high -- waiting up to 40s @09:32:13.24 SS.seek 25.00ml @09:32:14.49 Vacuum too high -- waiting up to 40s @09:32:14.96 Intake @43.5psia, vacuum from 20.0 to 24.0psi @09:32:14.97 SS.reconfigure forSampling @09:32:15.00 SS.seek 25.00ml @09:32:16.30 Vacuum too high -- waiting up to 40s @09:32:16.76 Intake @43.2psia, vacuum from 20.0 to 24.0psi @09:32:16.77 SS.reconfigure forSampling @09:32:17.42 SS.seek 25.00ml @09:32:19.12 Vacuum too high -- waiting up to 40s @09:32:19.60 Intake @43.3psia, vacuum from 20.0 to 24.0psi @09:32:19.61 SS.reconfigure forSampling @09:32:19.65 SS.seek 25.00ml @09:32:21.02 Vacuum too high -- waiting up to 40s @09:32:21.49 Intake @43.0psia, vacuum from 20.0 to 24.0psi @09:32:21.50 SS.reconfigure forSampling @09:32:21.53 SS.seek 25.00ml @09:32:22.80 Vacuum too high -- waiting up to 40s @09:32:23.28 Intake @43.3psia, vacuum from 20.0 to 24.0psi @09:32:23.29 SS.reconfigure forSampling @09:32:23.32 SS.seek 25.00ml @09:32:24.58 Vacuum too high -- waiting up to 40s @09:32:25.06 Intake @44.1psia, vacuum from 20.0 to 24.0psi @09:32:25.07 SS.reconfigure forSampling @09:32:25.10 SS.seek 25.00ml @09:32:25.96 Vacuum too high -- waiting up to 40s @09:32:26.43 Intake @44.3psia, vacuum from 20.0 to 24.0psi @09:32:26.44 SS.reconfigure forSampling @09:32:26.48 SS.seek 25.00ml @09:32:27.49 Vacuum too high -- waiting up to 40s @09:32:27.96 Intake @44.3psia, vacuum from 20.0 to 24.0psi @09:32:27.97 SS.reconfigure forSampling @09:32:28.00 SS.seek 25.00ml @09:32:29.02 Vacuum too high -- waiting up to 40s @09:32:29.49 Intake @44.6psia, vacuum from 20.0 to 24.0psi @09:32:29.50 SS.reconfigure forSampling @09:32:29.53 SS.seek 25.00ml @09:32:30.52 Vacuum too high -- waiting up to 40s @09:32:30.99 Intake @45.0psia, vacuum from 20.0 to 24.0psi @09:32:31.00 SS.reconfigure forSampling @09:32:31.03 SS.seek 25.00ml @09:32:31.96 Vacuum too high -- waiting up to 40s @09:32:32.22 Intake @44.9psia, vacuum from 20.0 to 24.0psi @09:32:32.23 SS.reconfigure forSampling @09:32:32.26 SS.seek 25.00ml @09:32:32.96 Vacuum too high -- waiting up to 40s @09:32:33.22 Intake @44.7psia, vacuum from 20.0 to 24.0psi @09:32:33.23 SS.reconfigure forSampling @09:32:33.26 SS.seek 25.00ml @09:32:34.05 Vacuum too high -- waiting up to 40s @09:32:34.31 Intake @44.6psia, vacuum from 20.0 to 24.0psi @09:32:34.32 SS.reconfigure forSampling @09:32:34.35 SS.seek 25.00ml @09:32:35.15 Vacuum too high -- waiting up to 40s @09:32:35.63 Intake @44.7psia, vacuum from 20.0 to 24.0psi @09:32:35.64 SS.reconfigure forSampling @09:32:35.67 SS.seek 25.00ml @09:32:36.61 Vacuum too high -- waiting up to 40s @09:32:36.87 Intake @44.6psia, vacuum from 20.0 to 24.0psi @09:32:36.88 SS.reconfigure forSampling @09:32:36.91 SS.seek 25.00ml @09:32:37.71 Vacuum too high -- waiting up to 40s @09:32:38.19 Intake @44.7psia, vacuum from 20.0 to 24.0psi @09:32:38.20 SS.reconfigure forSampling @09:32:38.23 SS.seek 25.00ml @09:32:39.15 Vacuum too high -- waiting up to 40s @09:32:39.62 Intake @44.5psia, vacuum from 20.0 to 24.0psi @09:32:39.63 SS.reconfigure forSampling @09:32:39.66 SS.seek 25.00ml @09:32:40.68 Vacuum too high -- waiting up to 40s @09:32:41.16 Intake @44.3psia, vacuum from 20.0 to 24.0psi @09:32:41.17 SS.reconfigure forSampling @09:32:41.20 SS.seek 25.00ml @09:32:42.36 Vacuum too high -- waiting up to 40s @09:32:42.83 Intake @44.7psia, vacuum from 20.0 to 24.0psi @09:32:42.84 SS.reconfigure forSampling @09:32:42.87 SS.seek 25.00ml @09:32:43.71 Vacuum too high -- waiting up to 40s @09:32:44.19 Intake @45.0psia, vacuum from 20.0 to 24.0psi @09:32:44.20 SS.reconfigure forSampling @09:32:44.24 SS.seek 25.00ml @09:32:45.27 Vacuum too high -- waiting up to 40s @09:32:45.76 Intake @44.7psia, vacuum from 20.0 to 24.0psi @09:32:45.77 SS.reconfigure forSampling @09:32:45.80 SS.seek 25.00ml @09:32:46.71 Vacuum too high -- waiting up to 40s @09:32:47.18 Intake @44.4psia, vacuum from 20.0 to 24.0psi @09:32:47.19 SS.reconfigure forSampling @09:32:47.22 SS.seek 25.00ml @09:32:48.98 Vacuum too high -- waiting up to 40s @09:32:49.24 Intake @44.7psia, vacuum from 20.0 to 24.0psi @09:32:49.25 SS.reconfigure forSampling @09:32:49.28 SS.seek 25.00ml @09:32:50.46 Vacuum too high -- waiting up to 40s @09:32:50.94 SS.seek 25.00ml @09:32:51.83 Vacuum too high -- waiting up to 40s @09:32:52.31 Intake @44.6psia, vacuum from 20.0 to 24.0psi @09:32:52.32 SS.reconfigure forSampling @09:32:52.35 SS.seek 25.00ml @09:32:53.33 Vacuum too high -- waiting up to 40s @09:32:53.80 Intake @44.4psia, vacuum from 20.0 to 24.0psi @09:32:53.81 SS.reconfigure forSampling @09:32:53.84 SS.seek 25.00ml @09:32:54.96 Vacuum too high -- waiting up to 40s @09:32:55.45 Intake @44.4psia, vacuum from 20.0 to 24.0psi @09:32:55.46 SS.reconfigure forSampling @09:32:55.50 SS.seek 25.00ml @09:32:56.58 Vacuum too high -- waiting up to 40s @09:32:57.05 Intake @44.6psia, vacuum from 20.0 to 24.0psi @09:32:57.06 SS.reconfigure forSampling @09:32:57.10 SS.seek 25.00ml @09:32:58.08 Vacuum too high -- waiting up to 40s @09:32:58.55 Intake @45.0psia, vacuum from 20.0 to 24.0psi @09:32:58.56 SS.reconfigure forSampling @09:32:58.59 SS.seek 25.00ml @09:32:59.43 Vacuum too high -- waiting up to 40s @09:32:59.90 Intake @44.9psia, vacuum from 20.0 to 24.0psi @09:32:59.91 SS.reconfigure forSampling @09:32:59.94 SS.seek 25.00ml @09:33:01.19 Vacuum too high -- waiting up to 40s @09:33:01.46 Intake @44.9psia, vacuum from 20.0 to 24.0psi @09:33:01.47 SS.reconfigure forSampling @09:33:01.50 SS.seek 25.00ml @09:33:02.52 Vacuum too high -- waiting up to 40s @09:33:03.00 Intake @44.7psia, vacuum from 20.0 to 24.0psi @09:33:03.01 SS.reconfigure forSampling @09:33:03.04 SS.seek 25.00ml @09:33:04.08 Vacuum too high -- waiting up to 40s @09:33:04.56 Intake @44.6psia, vacuum from 20.0 to 24.0psi @09:33:04.57 SS.reconfigure forSampling @09:33:04.61 SS.seek 25.00ml @09:33:05.58 Vacuum too high -- waiting up to 40s @09:33:06.07 Intake @44.4psia, vacuum from 20.0 to 24.0psi @09:33:06.08 SS.reconfigure forSampling @09:33:06.11 SS.seek 25.00ml @09:33:07.33 Vacuum too high -- waiting up to 40s @09:33:07.80 SS.seek 25.00ml @09:33:08.83 Vacuum too high -- waiting up to 40s @09:33:09.30 Intake @44.5psia, vacuum from 20.0 to 24.0psi @09:33:09.31 SS.reconfigure forSampling @09:33:09.34 SS.seek 25.00ml @09:33:10.46 Vacuum too high -- waiting up to 40s @09:33:10.95 Intake @45.0psia, vacuum from 20.0 to 24.0psi @09:33:10.96 SS.reconfigure forSampling @09:33:10.99 SS.seek 25.00ml @09:33:11.93 Vacuum too high -- waiting up to 40s @09:33:12.41 Intake @45.4psia, vacuum from 20.0 to 24.0psi @09:33:12.42 SS.reconfigure forSampling @09:33:12.45 SS.seek 25.00ml @09:33:13.30 Vacuum too high -- waiting up to 40s @09:33:13.56 Intake @45.1psia, vacuum from 20.0 to 24.0psi @09:33:13.57 SS.reconfigure forSampling @09:33:13.60 SS.seek 25.00ml @09:33:14.39 Vacuum too high -- waiting up to 40s @09:33:14.87 Intake @45.2psia, vacuum from 20.0 to 24.0psi @09:33:14.88 SS.reconfigure forSampling @09:33:14.91 SS.seek 25.00ml @09:33:15.89 Vacuum too high -- waiting up to 40s @09:33:16.37 Intake @44.7psia, vacuum from 20.0 to 24.0psi @09:33:16.38 SS.reconfigure forSampling @09:33:16.41 SS.seek 25.00ml @09:33:17.61 Vacuum too high -- waiting up to 40s @09:33:18.09 Intake @44.3psia, vacuum from 20.0 to 24.0psi @09:33:18.10 SS.reconfigure forSampling @09:33:18.13 SS.seek 25.00ml @09:33:19.33 Vacuum too high -- waiting up to 40s @09:33:20.37 Intake @44.1psia, vacuum from 20.0 to 24.0psi @09:33:20.38 SS.reconfigure forSampling @09:33:20.43 SS.seek 25.00ml @09:33:22.08 Vacuum too high -- waiting up to 40s @09:33:22.56 SS.seek 25.00ml @09:33:23.55 Vacuum too high -- waiting up to 40s @09:33:24.02 Intake @44.9psia, vacuum from 20.0 to 24.0psi @09:33:24.03 SS.reconfigure forSampling @09:33:24.07 SS.seek 25.00ml @09:33:24.96 Vacuum too high -- waiting up to 40s @09:33:25.45 Intake @45.4psia, vacuum from 20.0 to 24.0psi @09:33:25.46 SS.reconfigure forSampling @09:33:25.49 SS.seek 25.00ml @09:33:26.17 Vacuum too high -- waiting up to 40s @09:33:26.43 Intake @44.7psia, vacuum from 20.0 to 24.0psi @09:33:26.44 SS.reconfigure forSampling @09:33:26.48 SS.seek 25.00ml @09:33:27.42 Vacuum too high -- waiting up to 40s @09:33:27.89 Intake @44.3psia, vacuum from 20.0 to 24.0psi @09:33:27.90 SS.reconfigure forSampling @09:33:27.93 SS.seek 25.00ml @09:33:29.05 Vacuum too high -- waiting up to 40s @09:33:29.53 SS.seek 25.00ml @09:33:30.58 Vacuum too high -- waiting up to 40s @09:33:31.05 Intake @44.1psia, vacuum from 20.0 to 24.0psi @09:33:31.06 SS.reconfigure forSampling @09:33:31.09 SS.seek 25.00ml @09:33:32.27 Vacuum too high -- waiting up to 40s @09:33:32.95 Intake @44.1psia, vacuum from 20.0 to 24.0psi @09:33:32.96 SS.reconfigure forSampling @09:33:32.99 SS.seek 25.00ml @09:33:34.27 Vacuum too high -- waiting up to 40s @09:33:34.96 SS.seek 25.00ml @09:33:36.24 Vacuum too high -- waiting up to 40s @09:33:36.92 Intake @45.0psia, vacuum from 20.0 to 24.0psi @09:33:36.93 SS.reconfigure forSampling @09:33:36.96 SS.seek 25.00ml @09:33:38.17 Vacuum too high -- waiting up to 40s @09:33:38.64 Intake @44.8psia, vacuum from 20.0 to 24.0psi @09:33:38.65 SS.reconfigure forSampling @09:33:38.68 SS.seek 25.00ml @09:33:39.71 Vacuum too high -- waiting up to 40s @09:33:40.19 Intake @44.5psia, vacuum from 20.0 to 24.0psi @09:33:40.20 SS.reconfigure forSampling @09:33:40.23 SS.seek 25.00ml @09:33:41.24 Vacuum too high -- waiting up to 40s @09:33:41.72 SS.seek 25.00ml @09:33:42.58 Vacuum too high -- waiting up to 40s @09:33:43.05 Intake @44.1psia, vacuum from 20.0 to 24.0psi @09:33:43.06 SS.reconfigure forSampling @09:33:43.09 SS.seek 25.00ml @09:33:44.33 Vacuum too high -- waiting up to 40s @09:33:45.03 Intake @45.7psia, vacuum from 20.0 to 24.0psi @09:33:45.04 SS.reconfigure forSampling @09:33:45.07 SS.seek 25.00ml @09:33:45.96 Vacuum too high -- waiting up to 40s @09:33:46.43 Intake @46.0psia, vacuum from 20.0 to 24.0psi @09:33:46.44 SS.reconfigure forSampling @09:33:46.47 SS.seek 25.00ml @09:33:47.45 Vacuum too high -- waiting up to 40s @09:33:47.92 Intake @47.1psia, vacuum from 20.0 to 24.0psi @09:33:47.93 SS.reconfigure forSampling @09:33:47.97 SS.seek 25.00ml @09:33:48.80 Vacuum too high -- waiting up to 40s @09:33:49.06 Intake @47.4psia, vacuum from 20.0 to 24.0psi @09:33:49.07 SS.reconfigure forSampling @09:33:49.10 SS.seek 25.00ml @09:33:49.74 Vacuum too high -- waiting up to 40s @09:33:50.00 Intake @46.8psia, vacuum from 20.0 to 24.0psi @09:33:50.01 SS.reconfigure forSampling @09:33:50.04 SS.seek 25.00ml @09:33:50.70 Vacuum too high -- waiting up to 40s @09:33:51.58 Intake @46.5psia, vacuum from 20.0 to 24.0psi @09:33:51.59 SS.reconfigure forSampling @09:33:51.62 SS.seek 25.00ml @09:33:52.77 Vacuum too high -- waiting up to 40s @09:33:53.03 Intake @46.5psia, vacuum from 20.0 to 24.0psi @09:33:53.04 SS.reconfigure forSampling @09:33:53.08 SS.seek 25.00ml @09:33:53.74 Vacuum too high -- waiting up to 40s @09:33:54.22 Intake @46.1psia, vacuum from 20.0 to 24.0psi @09:33:54.23 SS.reconfigure forSampling @09:33:54.27 SS.seek 25.00ml @09:33:55.24 Vacuum too high -- waiting up to 40s @09:33:55.73 Intake @46.5psia, vacuum from 20.0 to 24.0psi @09:33:55.74 SS.reconfigure forSampling @09:33:55.78 SS.seek 25.00ml @09:33:56.74 Vacuum too high -- waiting up to 40s @09:33:57.22 Intake @46.7psia, vacuum from 20.0 to 24.0psi @09:33:57.23 SS.reconfigure forSampling @09:33:57.27 SS.seek 25.00ml @09:33:58.14 Vacuum too high -- waiting up to 40s @09:33:58.62 Intake @47.3psia, vacuum from 20.0 to 24.0psi @09:33:58.63 SS.reconfigure forSampling @09:33:58.67 SS.seek 25.00ml @09:33:59.45 Vacuum too high -- waiting up to 40s @09:33:59.71 Intake @47.6psia, vacuum from 20.0 to 24.0psi @09:33:59.72 SS.reconfigure forSampling @09:33:59.76 SS.seek 25.00ml @09:34:00.39 Vacuum too high -- waiting up to 40s @09:34:00.65 Intake @47.7psia, vacuum from 20.0 to 24.0psi @09:34:00.66 SS.reconfigure forSampling @09:34:00.69 SS.seek 25.00ml @09:34:01.39 Vacuum too high -- waiting up to 40s @09:34:01.65 Intake @47.6psia, vacuum from 20.0 to 24.0psi @09:34:01.66 SS.reconfigure forSampling @09:34:01.70 SS.seek 25.00ml @09:34:02.36 Vacuum too high -- waiting up to 40s @09:34:02.62 Intake @47.5psia, vacuum from 20.0 to 24.0psi @09:34:02.63 SS.reconfigure forSampling @09:34:02.67 SS.seek 25.00ml @09:34:03.33 Vacuum too high -- waiting up to 40s @09:34:03.81 Intake @47.3psia, vacuum from 20.0 to 24.0psi @09:34:03.82 SS.reconfigure forSampling @09:34:03.85 SS.seek 25.00ml @09:34:04.64 Vacuum too high -- waiting up to 40s @09:34:05.11 Intake @47.1psia, vacuum from 20.0 to 24.0psi @09:34:05.12 SS.reconfigure forSampling @09:34:05.15 SS.seek 25.00ml @09:34:06.02 Vacuum too high -- waiting up to 40s @09:34:06.50 Intake @46.8psia, vacuum from 20.0 to 24.0psi @09:34:06.51 SS.reconfigure forSampling @09:34:06.54 SS.seek 25.00ml @09:34:07.39 Vacuum too high -- waiting up to 40s @09:34:07.87 Intake @47.0psia, vacuum from 20.0 to 24.0psi @09:34:07.88 SS.reconfigure forSampling @09:34:07.91 SS.seek 25.00ml @09:34:08.74 Vacuum too high -- waiting up to 40s @09:34:09.00 Intake @47.4psia, vacuum from 20.0 to 24.0psi @09:34:09.01 SS.reconfigure forSampling @09:34:09.04 SS.seek 25.00ml @09:34:09.70 Vacuum too high -- waiting up to 40s @09:34:10.17 Intake @47.5psia, vacuum from 20.0 to 24.0psi @09:34:10.18 SS.reconfigure forSampling @09:34:10.21 SS.seek 25.00ml @09:34:11.05 Vacuum too high -- waiting up to 40s @09:34:11.53 Intake @47.3psia, vacuum from 20.0 to 24.0psi @09:34:11.54 SS.reconfigure forSampling @09:34:11.57 SS.seek 25.00ml @09:34:12.39 Vacuum too high -- waiting up to 40s @09:34:12.87 Intake @47.1psia, vacuum from 20.0 to 24.0psi @09:34:12.88 SS.reconfigure forSampling @09:34:12.91 SS.seek 25.00ml @09:34:13.80 Vacuum too high -- waiting up to 40s @09:34:14.06 Intake @47.2psia, vacuum from 20.0 to 24.0psi @09:34:14.07 SS.reconfigure forSampling @09:34:14.11 SS.seek 25.00ml @09:34:14.77 Vacuum too high -- waiting up to 40s @09:34:15.03 Intake @47.4psia, vacuum from 20.0 to 24.0psi @09:34:15.04 SS.reconfigure forSampling @09:34:15.07 SS.seek 25.00ml @09:34:15.67 Vacuum too high -- waiting up to 40s @09:34:16.15 Intake @47.4psia, vacuum from 20.0 to 24.0psi @09:34:16.16 SS.reconfigure forSampling @09:34:16.19 SS.seek 25.00ml @09:34:17.05 Vacuum too high -- waiting up to 40s @09:34:18.14 Intake @47.4psia, vacuum from 20.0 to 24.0psi @09:34:18.15 SS.reconfigure forSampling @09:34:18.18 SS.seek 25.00ml @09:34:19.39 Vacuum too high -- waiting up to 40s @09:34:19.87 Intake @47.1psia, vacuum from 20.0 to 24.0psi @09:34:19.88 SS.reconfigure forSampling @09:34:19.92 SS.seek 25.00ml @09:34:20.70 Vacuum too high -- waiting up to 40s @09:34:21.18 Intake @47.4psia, vacuum from 20.0 to 24.0psi @09:34:21.19 SS.reconfigure forSampling @09:34:21.23 SS.seek 25.00ml @09:34:22.02 Vacuum too high -- waiting up to 40s @09:34:22.50 Intake @48.1psia, vacuum from 20.0 to 24.0psi @09:34:22.51 SS.reconfigure forSampling @09:34:22.55 SS.seek 25.00ml @09:34:23.33 Vacuum too high -- waiting up to 40s @09:34:23.59 Intake @47.7psia, vacuum from 20.0 to 24.0psi @09:34:23.60 SS.reconfigure forSampling @09:34:23.64 SS.seek 25.00ml @09:34:24.23 Vacuum too high -- waiting up to 40s @09:34:24.49 Intake @47.3psia, vacuum from 20.0 to 24.0psi @09:34:24.50 SS.reconfigure forSampling @09:34:24.53 SS.seek 25.00ml @09:34:25.17 Vacuum too high -- waiting up to 40s @09:34:25.66 Intake @47.0psia, vacuum from 20.0 to 24.0psi @09:34:25.67 SS.reconfigure forSampling @09:34:25.70 SS.seek 25.00ml @09:34:26.61 Vacuum too high -- waiting up to 40s @09:34:27.08 Intake @46.9psia, vacuum from 20.0 to 24.0psi @09:34:27.09 SS.reconfigure forSampling @09:34:27.12 SS.seek 25.00ml @09:34:27.98 Vacuum too high -- waiting up to 40s @09:34:28.46 Intake @47.1psia, vacuum from 20.0 to 24.0psi @09:34:28.47 SS.reconfigure forSampling @09:34:28.51 SS.seek 25.00ml @09:34:29.30 Vacuum too high -- waiting up to 40s @09:34:29.56 Intake @47.1psia, vacuum from 20.0 to 24.0psi @09:34:29.57 SS.reconfigure forSampling @09:34:29.60 SS.seek 25.00ml @09:34:30.17 Vacuum too high -- waiting up to 40s @09:34:30.65 Intake @47.8psia, vacuum from 20.0 to 24.0psi @09:34:30.66 SS.reconfigure forSampling @09:34:30.69 SS.seek 25.00ml @09:34:31.54 Vacuum too high -- waiting up to 40s @09:34:31.81 Intake @48.0psia, vacuum from 20.0 to 24.0psi @09:34:31.82 SS.reconfigure forSampling @09:34:31.86 SS.seek 25.00ml @09:34:32.45 Vacuum too high -- waiting up to 40s @09:34:32.93 Intake @47.6psia, vacuum from 20.0 to 24.0psi @09:34:32.94 SS.reconfigure forSampling @09:34:32.97 SS.seek 25.00ml @09:34:33.80 Vacuum too high -- waiting up to 40s @09:34:34.39 Intake @46.9psia, vacuum from 20.0 to 24.0psi @09:34:34.40 SS.reconfigure forSampling @09:34:34.43 SS.seek 25.00ml @09:34:35.36 Vacuum too high -- waiting up to 40s @09:34:35.83 Intake @47.1psia, vacuum from 20.0 to 24.0psi @09:34:35.84 SS.reconfigure forSampling @09:34:35.87 SS.seek 25.00ml @09:34:36.70 Vacuum too high -- waiting up to 40s @09:34:37.18 Intake @46.9psia, vacuum from 20.0 to 24.0psi @09:34:37.19 SS.reconfigure forSampling @09:34:37.22 SS.seek 25.00ml @09:34:38.08 Vacuum too high -- waiting up to 40s @09:34:38.37 SS.reconfigure SSconfig @09:34:38.41 SS.hold @09:34:38.50 Intake.select :close @09:34:38.65 ShallowSampler::Clogged in MAIN -- progress too slow after 297.8ml SSV.dial :exhaust @09:34:39.45 Exhaust.select :open @09:34:39.55 SS.reconfigure SSexhale @09:34:39.59 SS.seek 0.00ml @09:34:47.81 Exhaust.select :close @09:34:47.91 Sampled 297.8ml @09:34:47.92 collectSample: Complete Evacuating Puck (bubblePt=55.0psi, delay=15.0s) @09:34:47.93 Intake.select :close @09:34:47.98 CSV.dial :waste @09:34:48.02 CS.seek 0.000ml @09:34:48.11 SSV.dial CBV,avoiding => exhaust @09:34:48.13
CBV.dial SSV @09:34:48.17 CSV.dial :air @09:34:49.22 CS.seek 10.000ml @09:34:50.13
CTV.dial :air @09:34:50.91 delay 15 seconds @09:34:50.93 Sleepy.power :sampler,:OFF @09:35:05.92
SSV.dial :spit,avoiding => exhaust @09:35:07.24 CTV.dial CSV,avoiding => intake @09:35:07.26
SS.seek 0.00ml @09:35:07.94 SSV.dial CBV,avoiding => exhaust @09:35:13.87 CBV.dial between CSV and waste2b @09:35:14.81 CTV.dial CSV,avoiding => air @09:35:14.83
CSV.dial CTV @09:35:15.36 CS.reconfigure CSpressurize @09:35:15.39 CS.seek 0.000ml @09:35:39.42 BubblePoint: Intake @70.3psia, Outlet @15.3psia @09:35:39.43 SS.reconfigure SSconfig @09:35:39.47 SS.seek 10.00ml @09:35:45.58 delay 15 seconds @09:36:00.68 Evacuated: Intake @69.7psia, Outlet @-0.0psia @09:36:00.69 SSV.dial :spit,avoiding => exhaust @09:36:02.00 SS.reconfigure SSexhale @09:36:02.03 SS.seek 0.00ml @09:36:06.99 SS.reconfigure SSfast @09:36:07.03 SS.seek 5.00ml @09:36:09.87 SSV.dial CBV,avoiding => exhaust @09:36:09.89
CBV.dial SSV @09:36:11.22 CTV.dial :air,avoiding => intake @09:36:12.53 CSV.dial CTV @09:36:12.54
SS.reconfigure SSconfig @09:36:12.58 SS.seek 10.00ml @09:36:13.54 CSV.dial :air @09:36:14.06 CS.reconfigure CSfast @09:36:14.09 CS.seek 0.000ml @09:36:15.59
SSV.dial :spit,avoiding => exhaust @09:36:16.60 CS.coast @09:36:16.92
SS.reconfigure SSexhale @09:36:16.95 SS.seek 0.00ml @09:36:21.97 SS.reconfigure SSfast @09:36:22.00 SS.seek 5.00ml @09:36:24.94 SSV.dial CBV,avoiding => exhaust @09:36:24.96
CBV.dial SSV @09:36:27.25 SSV.dial :spit,avoiding => exhaust @09:36:28.56 SS.reconfigure SSconfig @09:36:28.59 SS.seek 0.00ml @09:36:31.80 SS.coast @09:36:31.83 Evacuating Puck (bubblePt=55.0psi, delay=15.0s) Intake.select :close @09:36:31.89 CSV.dial :waste @09:36:31.97
SSV.dial :exhaust @09:36:32.65 CS.seek 0.000ml @09:36:32.71
Exhaust.select :open @09:36:32.81 SS.reconfigure SSexhale @09:36:32.85 SS.seek 0.00ml @09:36:32.91 CSV.dial :air @09:36:33.32
Exhaust.select :close @09:36:33.41 SSV.dial CBV,avoiding => exhaust @09:36:33.43
CBV.dial SSV @09:36:33.70 CS.seek 10.000ml @09:36:35.31
CTV.dial :air @09:36:35.32 delay 15 seconds @09:36:50.32 SSV.dial :spit,avoiding => exhaust @09:36:51.62 CTV.dial CSV,avoiding => intake @09:36:51.64
SS.seek 0.00ml @09:36:51.74 SSV.dial CBV,avoiding => exhaust @09:36:57.71 CBV.dial between CSV and waste2b @09:36:58.65 CSV.dial between air and CTV @09:36:58.96 CS.seek 7.483ml @09:37:05.54 CTV.dial CSV,avoiding => air @09:37:05.55
CSV.dial CTV @09:37:05.84 CS.reconfigure CSpressurize @09:37:05.87 CS.seek 0.000ml @09:37:25.34 CSV.dial :air @09:37:25.86 CS.reconfigure CSfast @09:37:25.89 CS.seek 10.000ml @09:37:49.54 CSV.dial between air and CTV @09:37:49.92 CS.seek 2.289ml @09:38:08.32 CTV.dial CSV,avoiding => air @09:38:08.33
CSV.dial CTV @09:38:08.62 CS.reconfigure CSpressurize @09:38:08.65 CS.seek 0.000ml @09:38:11.24 BubblePoint: Intake @73.6psia, Outlet @18.1psia @09:38:11.25 SS.reconfigure SSconfig @09:38:11.28 SS.seek 10.00ml @09:38:17.12 delay 15 seconds @09:38:32.22 Evacuated: Intake @72.6psia, Outlet @-0.0psia SSV.dial :spit,avoiding => exhaust @09:38:33.54 SS.reconfigure SSexhale @09:38:33.57 SS.seek 0.00ml @09:38:38.48 SS.reconfigure SSfast @09:38:38.51 SS.seek 5.00ml @09:38:41.40 SSV.dial CBV,avoiding => exhaust @09:38:41.42
CBV.dial SSV @09:38:42.76 CTV.dial :air,avoiding => intake @09:38:44.07 CSV.dial CTV @09:38:44.08
SS.reconfigure SSconfig @09:38:44.12 SS.seek 10.00ml @09:38:45.08 CSV.dial :air @09:38:45.59 CS.reconfigure CSfast @09:38:45.62 CS.seek 0.000ml @09:38:47.31
SSV.dial :spit,avoiding => exhaust @09:38:49.19 SS.reconfigure SSexhale @09:38:49.22 SS.seek 0.00ml @09:38:49.87 CS.coast @09:38:54.42
SS.reconfigure SSfast @09:38:54.46 SS.seek 5.00ml @09:38:57.57 SSV.dial CBV,avoiding => exhaust @09:38:57.59
CBV.dial SSV @09:38:59.90 SSV.dial :spit,avoiding => exhaust @09:39:01.21 SS.reconfigure SSconfig @09:39:01.24 SS.seek 0.00ml @09:39:04.31 SS.coast @09:39:04.34 Lysing: Adding 1.5ml lysis CBV.dial between CSV and waste1,avoiding => DBV @09:39:05.82 CTV.dial CSV @09:39:07.07 CSV.dial CSR @09:39:07.86 CSR.select :lysis @09:39:07.96 CS.reconfigure CSconfig @09:39:08.00 CS.seek 1.000ml @09:39:11.40 delay 5 seconds @09:39:16.41 CSR.select :air @09:39:16.55 CS.seek 6.000ml @09:39:29.96 delay 2 seconds @09:39:31.97 CSV.dial :waste1 @09:39:33.22 CS.reconfigure CSfast @09:39:33.25 CS.seek empty @09:39:47.90 CSV.dial CSR @09:39:49.17 CSR.select :lysis @09:39:49.27 CS.reconfigure CSconfig @09:39:49.31 CS.seek 1.500ml @09:39:53.90 delay 5 seconds @09:39:58.91 CSR.select :air @09:39:59.05 CS.seek 4.500ml @09:40:07.43 delay 2 seconds @09:40:09.44 CSV.dial CTV @09:40:09.96 CS.seek 1.275ml @09:40:19.00 Heating sh1 puck from tube 2 in Collection Clamp to 85.0C for 5:00 @09:40:19.01 CH.seek 85.0C,8:00 @09:43:11.38 CH.hold 5:00,85.0C @09:48:11.48 Collection Puck Heater at 84.9C after lysing @09:48:11.49 CS.seek 0.000ml @09:48:15.73 CTV.dial between spare and air,avoiding => intake @09:48:16.72 CSV.dial :air @09:48:17.15 CS.seek 5.000ml @09:48:30.66 CSV.dial CTV @09:48:31.16 delay 2 seconds @09:48:33.17 CSV.dial :air @09:48:33.67 delay 2 seconds @09:48:35.68 CSV.dial CTV @09:48:36.17 delay 2 seconds @09:48:38.18 CSV.dial :air @09:48:38.67 delay 2 seconds @09:48:40.68 CS.seek 0.000ml @09:48:54.22 Recovering Lysate into Collection syringe @09:48:54.23 CSV.dial CBV @09:48:55.22 CBV.dial CSV @09:48:55.58 CS.seek 4.999ml @09:49:09.14 delay 5 seconds @09:49:14.15 CTV.dial :air @09:49:14.62 CS.seek 7.000ml @09:49:20.51 delay 5 seconds @09:49:25.53 CSV.dial :air2 @09:49:25.96 CS.seek 10.000ml @09:49:34.29 CSV.dial between air and CTV,avoiding => CBV @09:49:35.41 Lysing Complete @09:49:35.46 Storing sh1 puck in Collection Clamp to tube 1 @09:49:35.59 SC.reconfigure SCtight @09:49:35.62 SC.seek 1 @09:49:37.89 SC.reconfigure SCloose @09:49:38.06 Elbow.reconfigure TightElbow @09:49:38.10 Elbow.seek 1 @09:49:39.72 Forearm.reconfigure TightForearm @09:49:39.75 Forearm.seek 1 + 800 counts @09:49:40.87 SE.reconfigure SEpick @09:49:40.92 SE.seek beamBreak @09:49:42.50 SE.configure SEconfig @09:49:42.62 Elbow.reconfigure LimpElbow @09:49:42.65 Forearm.reconfigure LooseForearm @09:49:42.69 Forearm.seek 1 @09:49:42.91 Elbow.hold @09:49:42.96 Elbow.reconfigure TightElbow @09:49:43.04 SE.seek 14.05 Pucks below Top Plate @09:49:43.51 Forearm.coast @09:49:43.54 Forearm.seek 1 + 345 counts @09:49:43.78 Forearm.reconfigure TightForearm @09:49:43.81 Forearm.seek 1 + 800 counts @09:49:44.05 SE.seek 15.05 Pucks below Top Plate @09:49:44.57 Forearm.reconfigure LooseForearm @09:49:44.61 Forearm.seek retract @09:49:45.53 Elbow.seek CC @09:49:47.05 Elbow.reconfigure LooseElbow @09:49:47.14 Forearm.seek CC @09:49:48.09 CC.seek sealed @09:49:49.01 Hand.grip @09:49:49.78 CC.seek open @09:49:53.11 Forearm.seek retract @09:49:54.71 Elbow.reconfigure TightElbow @09:49:54.75 Elbow.seek 1 @09:49:56.38 Elbow.reconfigure LimpElbow @09:49:56.42 Forearm.seek 1 @09:49:57.38 Elbow.hold @09:49:57.43 Elbow.reconfigure TightElbow @09:49:57.47 Hand.release @09:49:58.24 Forearm.coast @09:49:58.27 Forearm.seek 1 + 346 counts @09:49:58.51 Forearm.reconfigure TightForearm @09:49:58.54 Forearm.seek 1 + 800 counts @09:49:58.74 SE.seek down @09:49:59.84
Forearm.reconfigure LooseForearm @09:49:59.88 Forearm.seek retract @09:50:00.00 SE.coast @09:50:00.79
SC.coast @09:50:00.82 Tranferring lysate to PRV @09:50:00.83 CSV.dial :air @09:50:01.13 CS.seek 10.000ml @09:50:01.20 CSV.dial between air and CTV @09:50:01.60 AIV.dial :bypass @09:50:01.76 Beginning samplePassC @09:50:01.77 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @09:50:01.92 Elbow.seek FlushPuck::Garage @09:50:03.71 Elbow.reconfigure LooseElbow @09:50:03.79 Forearm.seek garage @09:50:04.76 Hand.grip @09:50:05.62 Forearm.seek retract @09:50:06.63 Elbow.reconfigure TightElbow @09:50:06.67 Elbow.seek CC @09:50:09.48 Elbow.reconfigure LooseElbow @09:50:09.57 Forearm.reconfigure TightForearm @09:50:09.60 Forearm.seek CC @09:50:10.70 CC.seek closed @09:50:14.46 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.97 @09:50:14.51 Hand.release @09:50:15.32 Forearm.reconfigure LooseForearm @09:50:15.36 Forearm.seek retract @09:50:16.30 AWV.select :air @09:50:16.31 AIV.dial :bypass @09:50:16.32 PSV.dial PSR @09:50:17.60 PSR.select :flush @09:50:17.70 PS.seek 1.500ml @09:50:33.83 delay 5 seconds @09:50:38.84 PSV.dial :waste1 @09:50:39.28 PS.seek 0.000ml @09:50:55.33 PSV.dial PSR @09:50:55.77 PSR.select :air @09:50:55.87 PS.seek 2.500ml @09:51:22.06 CBV.dial PRV @09:51:22.07 PRV.dial CBV @09:51:22.82
PSV.dial :mixloop @09:51:23.82 CTV.dial :air,avoiding => intake @09:51:23.83 PTV.dial :PRVmixing @09:51:23.91 PS.seek 0.000ml @09:51:50.18 PSV.dial :air @09:51:50.75 PS.seek 2.500ml @09:52:17.02 PSV.dial :mixloop @09:52:17.45 PS.seek 0.000ml @09:52:43.86 PSV.dial :air @09:52:44.31 PS.seek 2.500ml @09:53:10.48 PSV.dial :mixloop @09:53:10.93 PS.seek 0.000ml @09:53:37.39 Garaging FlushPuck in Collection Clamp @09:53:37.45 Elbow.reconfigure TightElbow @09:53:37.48 Elbow.seek CC @09:53:37.71 Elbow.reconfigure LooseElbow @09:53:37.80 Forearm.seek CC @09:53:38.74 CC.seek sealed @09:53:39.69 Hand.grip @09:53:40.97 CC.seek open @09:53:44.28 Forearm.seek retract @09:53:45.21 Elbow.reconfigure TightElbow @09:53:45.25 Elbow.seek garage @09:53:48.01 Elbow.reconfigure LooseElbow @09:53:48.09 Forearm.reconfigure TightForearm @09:53:48.12 Forearm.seek garage @09:53:49.26 Hand.release @09:53:50.06 Forearm.reconfigure LooseForearm @09:53:50.09 Forearm.seek retract @09:53:51.12 Connecting PS to CS to transfer sample @09:53:51.13 CBV.dial AIV @09:53:51.14 AIV.dial :bypass @09:53:51.15 PSV.dial :mixloop @09:53:51.16 PTV.dial :PRVmixing @09:53:51.17 PRV.dial AIV @09:53:51.18 CTV.dial :air,avoiding => intake @09:53:51.19 Loading lyfil puck from tube 2 into Collection Clamp @09:53:51.36 SC.reconfigure SCtight @09:53:51.39 SC.seek 2 @09:53:53.50 SC.reconfigure SCloose @09:53:53.68 Elbow.reconfigure TightElbow @09:53:53.71 Elbow.seek 2 @09:53:55.57 Forearm.reconfigure TightForearm @09:53:55.61 Forearm.seek 2 + 800 counts @09:53:56.62 SE.reconfigure SEpick @09:53:56.67 SE.seek beamBreak @09:53:59.07 SE.configure SEconfig @09:53:59.19 Elbow.seek 2 @09:53:59.41 Forearm.seek 2 + 300 counts @09:53:59.73 Forearm.reconfigure LooseForearm @09:53:59.77 Hand.grip @09:54:00.53 SE.seek down @09:54:00.67
Forearm.seek retract @09:54:01.61 Elbow.seek CC @09:54:02.78 SE.coast @09:54:03.08
Elbow.reconfigure LooseElbow @09:54:03.17 Forearm.reconfigure TightForearm @09:54:03.20 Forearm.seek CC @09:54:04.28 CC.seek closed @09:54:08.05 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 2.00 @09:54:08.06 Hand.release @09:54:08.81 Forearm.reconfigure LooseForearm @09:54:08.84 Forearm.seek retract @09:54:09.78 SC.coast @09:54:09.81 CTV.dial CSV,avoiding => intake @09:54:11.11 CSV.dial CTV @09:54:11.40 CS.seek 5.000ml @09:54:24.87 delay 1:20 @09:55:44.88 CS.seek 2.500ml @09:55:52.05 delay 40 seconds @09:56:32.06 CS.seek 0.000ml @09:56:39.18 delay 1:00 @09:57:39.19 PSV.dial :air @09:57:39.64 PS.seek 2.500ml @09:58:05.88 PSV.dial :mixloop @09:58:06.32 delay 3 seconds @09:58:09.33 PSV.dial :air @09:58:09.77 delay 2 seconds @09:58:11.78 PSV.dial :mixloop @09:58:12.22 delay 3 seconds @09:58:15.23 PSV.dial :waste1 @09:58:16.50 PS.seek empty @09:58:42.78 CBV.dial :waste1 @09:58:43.23 delay 5 seconds @09:58:48.24 CBV.dial AIV @09:58:48.69 delay 10 seconds @09:58:58.70 CBV.dial :waste1 @09:58:59.17 delay 10 seconds @09:59:09.18 CBV.dial AIV @09:59:09.64 delay 10 seconds @09:59:19.65 CTV.dial CSV,avoiding => intake @09:59:19.66 CBV.dial between AIV and waste1 @09:59:19.98 CSV.dial :air @09:59:20.49 CS.seek 10.000ml @09:59:46.64 CSV.dial CTV @09:59:47.15 CS.seek 0.000ml @10:00:13.23 CBV.dial :waste1 @10:00:13.53 delay 10 seconds @10:00:23.54 CTV.dial :air,avoiding => intake @10:00:24.89 Storing lyfil puck in Collection Clamp to tube 1 @10:00:25.01 SC.reconfigure SCtight @10:00:25.04 SC.seek 1 @10:00:27.36 SC.reconfigure SCloose @10:00:28.15 Elbow.reconfigure TightElbow @10:00:28.19 Elbow.seek 1 @10:00:29.79 Forearm.reconfigure TightForearm @10:00:29.82 Forearm.seek 1 + 800 counts @10:00:30.93 SE.reconfigure SEpick @10:00:30.98 SE.seek beamBreak @10:00:32.62 SE.configure SEconfig @10:00:32.74 Elbow.reconfigure LimpElbow @10:00:32.78 Forearm.reconfigure LooseForearm @10:00:32.82 Forearm.seek 1 @10:00:33.06 Elbow.hold @10:00:33.12 Elbow.reconfigure TightElbow @10:00:33.20 SE.seek 15.05 Pucks below Top Plate @10:00:33.53 Forearm.coast @10:00:33.57 Forearm.seek 1 + 342 counts @10:00:33.81 Forearm.reconfigure TightForearm @10:00:33.84 Forearm.seek 1 + 800 counts @10:00:34.08 SE.seek 16.05 Pucks below Top Plate @10:00:34.63 Forearm.reconfigure LooseForearm @10:00:34.66 Forearm.seek retract @10:00:35.63 Elbow.seek CC @10:00:37.25 Elbow.reconfigure LooseElbow @10:00:37.34 Forearm.seek CC @10:00:38.28 CC.seek sealed @10:00:39.20 Hand.grip @10:00:39.88 CC.seek open @10:00:43.23 Forearm.seek retract @10:00:44.16 Elbow.reconfigure TightElbow @10:00:44.20 Elbow.seek 1 @10:00:45.80 Elbow.reconfigure LimpElbow @10:00:45.83 Forearm.seek 1 @10:00:46.80 Elbow.hold @10:00:46.85 Elbow.reconfigure TightElbow @10:00:46.89 Hand.release @10:00:47.71 Forearm.coast @10:00:47.74 Forearm.seek 1 + 335 counts @10:00:48.02 Forearm.reconfigure TightForearm @10:00:48.05 Forearm.seek 1 + 800 counts @10:00:48.34 SE.seek down @10:00:49.45
Forearm.reconfigure LooseForearm @10:00:49.48 Forearm.seek retract @10:00:49.55 SE.coast @10:00:50.41
SC.coast @10:00:50.44 PSV.dial :air @10:00:51.49 PS.seek 0.499ml @10:00:57.50 delay 3 seconds @10:01:00.52 PSV.dial :mixloop @10:01:00.95 PRV.dial :air @10:01:01.40 delay 3 seconds @10:01:04.41 PRV.dial CBV @10:01:04.86 CBV.dial AIV @10:01:05.32 PRV.dial between CBV and air @10:01:05.64 PRV.dial :air @10:01:05.98 PS.reconfigure PSslow @10:01:06.01 PS.seek 0.599ml @10:01:08.50 PRV.dial :waste2 @10:01:09.23 PS.reconfigure PSconfig @10:01:09.26 PS.seek empty @10:01:16.18 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @10:01:16.33 Elbow.seek FlushPuck::Garage @10:01:18.08 Elbow.reconfigure LooseElbow @10:01:18.17 Forearm.seek garage @10:01:19.18 Hand.grip @10:01:19.97 Forearm.seek retract @10:01:20.99 Elbow.reconfigure TightElbow @10:01:21.03 Elbow.seek CC @10:01:23.86 Elbow.reconfigure LooseElbow @10:01:23.95 Forearm.reconfigure TightForearm @10:01:23.98 Forearm.seek CC @10:01:25.10 CC.seek closed @10:01:28.91 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 2.02 @10:01:28.96 Hand.release @10:01:29.79 Forearm.reconfigure LooseForearm @10:01:29.82 Forearm.seek retract @10:01:30.79 Clearing PML for modified lysate @10:01:30.80 CTV.dial :air,avoiding => intake PRV.dial :waste2 @10:01:30.81 PTV.dial :PRV,avoiding => puck @10:01:31.52 delay 2 seconds @10:01:33.52 PTV.dial :PRVmixing,avoiding => puck @10:01:34.25 delay 2 seconds @10:01:36.26 PSV.dial :PML @10:01:36.27 delay 2 seconds @10:01:38.27 PRV.dial :air @10:01:38.98 delay 2 seconds @10:01:41.04 PS.seek 1.499ml @10:01:57.15 delay 5 seconds @10:02:02.16 PS.seek 2.500ml @10:02:13.18 PSV.dial :air @10:02:13.75 PS.seek 0.000ml @10:02:40.02 mixing lysate with speDil @10:02:40.03 PSV.dial :PML @10:02:40.45 CBV.dial :PRV @10:02:40.46 PRV.dial :air @10:02:40.47 delay 2 seconds @10:02:42.48 PRV.dial :speDil @10:02:42.93 delay 2 seconds @10:02:44.99 PS.reconfigure PSslow1 @10:02:45.02 PS.seek 0.225ml @10:03:08.46 delay 5 seconds @10:03:13.47 PRV.dial CBV @10:03:14.21 delay 2 seconds @10:03:16.27 PS.seek 0.475ml @10:03:42.15 delay 5 seconds @10:03:47.16 PRV.dial :air @10:03:47.60 delay 2 seconds @10:03:49.61 PS.seek 0.925ml @10:04:35.71 delay 10 seconds @10:04:45.72 PRV.dial CBV @10:04:46.18 CTV.dial :air,avoiding => intake @10:04:46.19 PSV.dial :air @10:04:46.64 PS.reconfigure PSconfig @10:04:46.67 PS.seek 2.500ml @10:05:03.46 delay 3 seconds @10:05:06.47 PSV.dial PTV @10:05:07.19 PTV.dial :PRV,avoiding => puck @10:05:07.92 PS.seek 0.000ml @10:05:34.21 PSV.dial :air @10:05:34.93 PS.seek 2.500ml @10:06:01.24 delay 3 seconds @10:06:04.25 PSV.dial PTV @10:06:04.97 PTV.dial :PRV,avoiding => puck @10:06:04.99 PS.seek 0.000ml @10:06:31.21 Pushing modified lysate into AIV Injection Loop @10:06:31.22 CBV.dial AIV @10:06:31.23 AIV.dial :bypass @10:06:31.24 PRV.dial CBV @10:06:31.25 PSV.dial :air @10:06:31.97 PS.seek 2.500ml @10:06:58.24 delay 3 seconds @10:07:01.25 PSV.dial :PML @10:07:01.68 PTV.dial :PRVmixing,avoiding => puck @10:07:02.45 PS.reconfigure PSslow1 @10:07:02.48 PS.seek 1.850ml @10:08:08.90 delay 5 seconds @10:08:13.91 AIV.dial :sample @10:08:14.55 AWV.select :waste @10:08:14.71 PS.reconfigure PSslow2 @10:08:14.74 PS.seek 1.310ml @10:10:04.74 delay 15 seconds @10:10:19.75 AIV.dial :bypass @10:10:20.29 PS.reconfigure PSconfig @10:10:20.32 PS.seek 0.000ml @10:10:34.55 PSV.dial :air @10:10:34.99 PS.seek 2.500ml @10:11:01.17 PSV.dial :PML @10:11:01.62 PS.seek 0.000ml @10:11:27.86 AIV.dial :coilToMFB @10:11:27.87 AWV.select :air @10:11:28.01 Extracting Nucleic Acid from AIV @10:11:28.03
Beginning samplePassD @10:11:28.04 SPE.hold 2:46:40,55.0C @10:11:28.06 ASV.dial :SPEdelivery @10:11:28.19
AWV.select :air @10:11:28.20 AIV.dial :bypass PSV.dial PSR @10:11:28.89 delay 20 seconds @10:11:29.34
PSR.select :killMFB @10:11:29.44 PS.seek 1.500ml @10:11:45.54 delay 5 seconds @10:11:48.90 primeLysateINT @10:11:48.91 AWV.select :air @10:11:48.92 AIV.dial :coilToMFB ATV.dial :AIV @10:11:50.26 AS.reconfigure ASslow1 @10:11:50.30 AS.seek 0.239ml @10:11:50.55
PSV.dial :waste1 @10:11:51.01 PS.seek 0.000ml @10:12:07.11 PSV.dial PSR @10:12:07.73 PSR.select :air @10:12:07.82 PS.seek 2.500ml @10:12:34.10 PRV.dial CBV @10:12:34.12
CBV.dial PRV @10:12:34.13 PSV.dial :mixloop @10:12:35.17 CTV.dial :air,avoiding => intake @10:12:35.18 PTV.dial :PRVmixing @10:12:35.20 PS.seek 0.000ml @10:12:39.52 delay 10 seconds @10:12:49.53 ATV.dial :air2 @10:12:50.10 AS.seek 0.269ml @10:12:56.38 ATV.dial :waste2 @10:12:56.74 AS.reconfigure ASnorm @10:12:56.77 AS.seek empty @10:13:01.48
PSV.dial PSR @10:13:02.51 PSR.select :flush @10:13:02.61 PS.seek 1.500ml @10:13:10.94 Lysate primed! @10:13:10.95 AFV.select :holdingCoil @10:13:10.96 ATV.dial :air2 @10:13:11.35 AS.reconfigure ASslow1 @10:13:11.38 AS.seek 0.069ml @10:13:18.70
delay 5 seconds @10:13:23.71 PSV.dial :waste1 @10:13:24.16 PS.seek 0.000ml @10:13:25.99 delay 3 seconds @10:13:29.00 ATV.dial :AIV @10:13:29.67 AS.seek 0.469ml @10:13:40.26
PSV.dial PSR @10:13:40.71 PSR.select :air @10:13:40.81 PS.seek 2.500ml @10:14:07.10 PRV.dial CBV @10:14:07.12
CBV.dial PRV @10:14:07.13 PSV.dial :mixloop @10:14:08.15 CTV.dial :air,avoiding => intake @10:14:08.16 PTV.dial :PRVmixing @10:14:08.18 PS.seek 0.000ml @10:14:34.57 PSV.dial PSR @10:14:35.58 PSR.select :flush @10:14:35.68 PS.seek 1.500ml @10:14:51.50 delay 5 seconds @10:14:51.79
delay 5 seconds @10:14:56.55 Solid Phase Extraction Heater at 52.4C @10:14:56.56 ATV.dial ASV @10:14:56.80
PSV.dial :waste1 @10:14:56.91 ASV.dial :SPEdelivery @10:14:56.97 AS.seek 0.041ml @10:14:57.25
PS.seek 0.000ml @10:15:13.29 PSV.dial PSR @10:15:13.74 PSR.select :air @10:15:13.84 PS.seek 2.500ml @10:15:40.19 PRV.dial CBV @10:15:40.21
CBV.dial PRV @10:15:40.22 PSV.dial :mixloop @10:15:41.22 CTV.dial :air,avoiding => intake @10:15:41.23 PTV.dial :PRVmixing @10:15:41.25 PS.seek 0.000ml @10:16:07.50 PSV.dial PSR @10:16:08.52 PSR.select :flush @10:16:08.62 PS.seek 1.500ml @10:16:24.52 delay 10 seconds @10:16:24.69
delay 5 seconds @10:16:29.70 PSV.dial :waste1 @10:16:30.14 PS.seek 0.000ml @10:16:34.53 rinseLysateLineINT @10:16:34.54 AFV.select :carrierSolution @10:16:34.63 delay 2 seconds @10:16:36.64 AS.reconfigure ASconfig @10:16:36.67 AS.seek 1.000ml @10:16:46.19
PSV.dial PSR @10:16:46.63 PSR.select :air @10:16:46.73 PS.seek 2.500ml @10:17:12.97 PRV.dial CBV @10:17:12.99
CBV.dial PRV @10:17:13.00 PSV.dial :mixloop @10:17:14.00 CTV.dial :air,avoiding => intake @10:17:14.01 PTV.dial :PRVmixing @10:17:14.03 PS.reconfigure PSslow @10:17:14.06 PS.seek 1.200ml @10:17:32.87 delay 1 second @10:17:33.88 AFV.select :holdingCoil @10:17:33.97 delay 1 second @10:17:34.98 ATV.dial :AIV @10:17:35.33 AIV.dial :coilToMFB @10:17:35.34 AWV.select :waste @10:17:35.45 AS.reconfigure ASslow0 @10:17:35.48 AS.seek 0.000ml @10:17:40.94
PSV.dial :waste1 @10:17:42.24 PS.reconfigure PSconfig @10:17:42.27 PS.seek 0.000ml @10:17:55.28 PSV.dial :air @10:17:56.30 PS.seek 2.500ml @10:18:22.47 PSV.dial :mixloop @10:18:22.91 PS.reconfigure PSslow @10:18:22.94 PS.seek 0.000ml @10:19:14.31 PSV.dial :air @10:19:14.77 PS.reconfigure PSconfig @10:19:14.80 PS.seek 2.500ml @10:19:17.79 ATV.dial :air2 @10:19:18.36 AS.reconfigure ASslow1 @10:19:18.39 AS.seek 0.050ml @10:19:28.96 ATV.dial :AIV @10:19:29.54 AS.seek 0.010ml @10:19:38.12 ATV.dial :waste @10:19:39.41 AS.seek empty @10:19:40.99
PSV.dial :mixloop @10:19:41.44 PS.reconfigure PSslow @10:19:41.47 PS.seek 0.000ml @10:19:41.90 ethanol wash @10:19:41.91 ATV.dial :air2 @10:19:43.39 AS.reconfigure ASslow2 @10:19:43.42 AS.seek 0.009ml @10:19:54.29 delay 3 seconds @10:19:57.30 Pulling 0.4ml of Ethanol Wash @10:19:57.31 ATV.dial :ethanol @10:19:57.87 AS.reconfigure ASslow1 @10:19:57.90 AS.seek 0.409ml @10:20:32.84
Cleaning Collection Syringe @10:20:32.85 CSV.dial CSR @10:20:33.28 CSR.select :flush @10:20:33.38 CS.seek 4.000ml @10:20:44.34 delay 5 seconds @10:20:49.35 CSR.select :air @10:20:49.44 CS.seek 10.000ml @10:21:05.43 CSV.dial CTV @10:21:05.45
CTV.dial CSV @10:21:06.78 CBV.dial :waste1 @10:21:07.27 CS.seek 0.000ml @10:21:19.79 delay 3 seconds @10:21:22.80 ATV.dial :air1 @10:21:23.13 delay 3 seconds @10:21:26.19 AS.seek 0.434ml @10:21:31.55 delay 3 seconds @10:21:33.43
CSV.dial CSR @10:21:33.93 CSR.select :flush @10:21:34.03 CS.seek 4.000ml @10:21:34.56 ATV.dial :ethanol @10:21:34.93 AS.seek 0.419ml @10:21:38.22 delay 3 seconds @10:21:41.23 ATV.dial ASV @10:21:42.37 AS.seek 0.092ml @10:21:45.06
delay 5 seconds @10:21:50.07 CSR.select :air @10:21:50.16 CS.seek 10.000ml @10:22:06.21 CSV.dial CTV @10:22:06.23
CTV.dial CSV @10:22:06.74 CBV.dial :waste1 @10:22:06.76 CS.seek 0.000ml @10:22:32.89 Garaging FlushPuck in Collection Clamp @10:22:32.94 Elbow.reconfigure TightElbow @10:22:32.97 Elbow.seek CC @10:22:33.18 Elbow.reconfigure LooseElbow @10:22:33.26 Forearm.seek CC @10:22:34.22 CC.seek sealed @10:22:35.15 Hand.grip @10:22:35.83 CC.seek open @10:22:39.14 Forearm.seek retract @10:22:40.08 Elbow.reconfigure TightElbow @10:22:40.12 Elbow.seek garage @10:22:42.91 Elbow.reconfigure LooseElbow @10:22:42.99 Forearm.reconfigure TightForearm @10:22:43.02 Forearm.seek garage @10:22:44.18 Hand.release @10:22:44.99 Forearm.reconfigure LooseForearm @10:22:45.02 Forearm.seek retract @10:22:46.08 Whole Cell Archive sampling 1000ml @10:22:46.10 Loading wcr puck from tube 2 into Collection Clamp @10:22:46.27 SC.reconfigure SCtight @10:22:46.31 SC.seek 2 @10:22:48.59 SC.reconfigure SCloose @10:22:48.77 Elbow.reconfigure TightElbow @10:22:48.80 Elbow.seek 2 @10:22:49.30 delay 3 seconds @10:22:50.73 Forearm.reconfigure TightForearm @10:22:50.76 Forearm.seek 2 + 800 counts @10:22:51.79 SE.reconfigure SEpick @10:22:51.85 SE.seek beamBreak @10:22:52.31 ATV.dial :waste2 @10:22:52.95 AS.reconfigure ASconfig @10:22:52.98 AS.seek empty @10:22:54.46 SE.configure SEconfig @10:22:54.59 Elbow.seek 2 @10:22:54.82 Forearm.seek 2 + 300 counts @10:22:55.04 Forearm.reconfigure LooseForearm @10:22:55.08 Hand.grip @10:22:55.85 SE.seek down @10:22:55.98 Forearm.seek retract @10:22:56.95 Elbow.seek CC @10:22:58.21 SE.coast @10:22:58.56 Elbow.reconfigure LooseElbow @10:22:59.24 ATV.dial :air2 @10:22:59.30 Forearm.reconfigure TightForearm @10:22:59.34 Forearm.seek CC @10:22:59.61 AS.reconfigure ASslow1 @10:22:59.64 AS.seek 1.000ml @10:23:00.55 CC.seek closed @10:23:04.32 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.96 @10:23:04.33 Hand.release @10:23:05.12 Forearm.reconfigure LooseForearm @10:23:05.16 Forearm.seek retract @10:23:06.10 SC.coast @10:23:06.13 collectSample 1000ml w/bubble point of 55psi @10:23:06.15 Priming 7ml Sleepy.power :sampler,:ON @10:23:06.16 Intake.select :close @10:23:06.17 Exhaust.select :close @10:23:06.18 SS.hold @10:23:06.34 Calibrating pressure sensors @10:23:06.35 CTV.dial :air @10:23:07.66 SSV.dial :spit,avoiding => exhaust @10:23:07.67 SS.reconfigure SSfast @10:23:07.70 SS.seek 5.00ml @10:23:10.51 SSV.dial CBV @10:23:10.53 CBV.dial SSV @10:23:11.93 delay 3.8 seconds @10:23:15.82 SSV.dial :spit @10:23:17.18 SS.seek 0.00ml @10:23:19.93 SSV.dial CBV @10:23:21.26 CTV.dial :intake @10:23:22.00 Waiting for external sampler to finish priming... @10:23:22.01 delayUntil "10:33:06.15" @10:26:24.08 delay 5 seconds @10:26:29.09 ATV.dial ASV @10:26:29.90 ASV.dial :SPEdelivery @10:26:29.92 AS.seek 0.020ml @10:29:50.06 delay 5 seconds @10:29:55.07 ATV.dial :air2 @10:29:55.82 AS.seek 0.520ml @10:31:38.06 delay 5 seconds @10:31:43.07 ATV.dial ASV @10:31:43.79 ASV.dial :wasteR @10:31:44.27 AS.seek 0.300ml @10:32:29.42 delay 20 seconds @10:32:49.43 AS.seek 0.070ml @10:33:06.16 SSV.dial CBV @10:33:06.18 CBV.dial SSV @10:33:06.19 Allowing 55s for 7.0ml of remaining 7.0ml @10:33:06.20 Intake.select :open @10:33:08.33 Intake @49.7psia, vacuum from 20.0 to 24.0psi @10:33:08.35 SS.reconfigure forSampling @10:33:08.38 SS.seek 7.00ml @10:33:14.39 Stroke completed -- waiting for pressure to equalize ... @10:33:15.03 Intake.select :close @10:33:15.16 SSV.dial :exhaust @10:33:15.95 Exhaust.select :open @10:33:16.05 SS.reconfigure SSexhale @10:33:16.09 SS.seek 0.00ml @10:33:19.46 Exhaust.select :close @10:33:19.57 Sampling 1000.0ml @10:33:19.58 Intake.select :close @10:33:19.59 Exhaust.select :close @10:33:19.60 SS.reconfigure SSconfig @10:33:19.63 SS.hold @10:33:19.79 Calibrating pressure and temperature sensors @10:33:19.81 CTV.dial :air @10:33:20.59 SSV.dial :spit,avoiding => exhaust @10:33:21.36 SS.reconfigure SSfast @10:33:21.39 SS.seek 5.00ml @10:33:24.54 SSV.dial CBV @10:33:24.56 CBV.dial SSV @10:33:25.94 delay 3.8 seconds @10:33:29.83 SSV.dial :spit @10:33:31.21 SS.seek 0.00ml @10:33:33.77 SSV.dial CBV @10:33:35.13 CTV.dial :intake @10:33:35.92 SSV.dial CBV @10:33:35.93 CBV.dial SSV @10:33:35.94 Allowing 185s for 25.0ml of remaining 1000.0ml @10:33:35.95 Intake.select :open @10:33:36.59 delay 20 seconds @10:33:38.08 Intake @48.8psia, vacuum from 20.0 to 24.0psi @10:33:38.10 SS.reconfigure forSampling @10:33:38.13 SS.seek 25.00ml @10:33:53.27 Stroke completed -- waiting for pressure to equalize ... @10:33:53.91 Intake.select :close @10:33:54.05 SSV.dial :exhaust @10:33:54.85 Exhaust.select :open @10:33:54.95 SS.reconfigure SSexhale @10:33:54.99 SS.seek 0.00ml @10:33:56.60 ATV.dial :waste2 @10:33:57.25 AS.reconfigure ASconfig @10:33:57.29 AS.seek empty @10:34:01.71 Elution @10:34:01.72 ATV.dial :air1 @10:34:02.70 AS.reconfigure ASslow1 @10:34:02.73 AS.seek 0.049ml @10:34:03.88 Exhaust.select :close @10:34:03.98 SSV.dial CBV @10:34:04.00 CBV.dial SSV @10:34:04.81 Allowing 185s for 25.0ml of remaining 975.0ml @10:34:04.82 Intake.select :open @10:34:06.95 Intake @48.0psia, vacuum from 20.0 to 24.0psi @10:34:06.97 SS.reconfigure forSampling @10:34:07.00 SS.seek 25.00ml @10:34:13.33 delay 3 seconds @10:34:15.26 Vacuum too high -- waiting up to 40s @10:34:15.54 Intake @40.9psia, vacuum from 20.0 to 24.0psi @10:34:15.56 SS.reconfigure forSampling @10:34:15.59 SS.seek 25.00ml @10:34:16.34 ATV.dial :ElutionWater @10:34:16.92 AS.seek 0.109ml @10:34:22.94 Stroke completed -- waiting for pressure to equalize ... @10:34:23.58 Intake.select :close @10:34:23.72 SSV.dial :exhaust @10:34:24.51 Exhaust.select :open @10:34:24.61 SS.reconfigure SSexhale @10:34:24.65 SS.seek 0.00ml @10:34:29.47 delay 3 seconds @10:34:32.48 ATV.dial ASV,via => air2 @10:34:33.44 Exhaust.select :close @10:34:33.54 SSV.dial CBV @10:34:33.56 CBV.dial SSV @10:34:34.16 ASV.dial :SPEelution @10:34:34.38 Allowing 185s for 25.0ml of remaining 950.0ml @10:34:34.39 Intake.select :open @10:34:35.01 AFV.select :carrierSolution @10:34:35.16 AS.reconfigure ASnorm @10:34:35.19 AS.seek 0.759ml @10:34:36.52 Intake @47.7psia, vacuum from 20.0 to 24.0psi @10:34:36.54 SS.reconfigure forSampling @10:34:36.57 SS.seek 25.00ml @10:34:39.63 Vacuum too high -- waiting up to 40s @10:34:39.90 Intake @42.3psia, vacuum from 20.0 to 24.0psi @10:34:39.91 SS.reconfigure forSampling @10:34:39.95 SS.seek 25.00ml @10:34:44.25 Vacuum too high -- waiting up to 40s @10:34:44.73 Intake @45.0psia, vacuum from 20.0 to 24.0psi @10:34:44.75 SS.reconfigure forSampling @10:34:44.78 SS.seek 25.00ml @10:34:48.16 Vacuum too high -- waiting up to 40s @10:34:48.65 Intake @46.3psia, vacuum from 20.0 to 24.0psi @10:34:48.67 SS.reconfigure forSampling @10:34:48.70 SS.seek 25.00ml @10:34:51.75 Vacuum too high -- waiting up to 40s @10:34:52.02 Intake @44.4psia, vacuum from 20.0 to 24.0psi @10:34:52.03 SS.reconfigure forSampling @10:34:52.07 SS.seek 25.00ml @10:34:56.88 Vacuum too high -- waiting up to 40s @10:34:57.36 Intake @46.3psia, vacuum from 20.0 to 24.0psi @10:34:57.38 SS.reconfigure forSampling @10:34:57.41 SS.seek 25.00ml @10:35:04.02 Stroke completed -- waiting for pressure to equalize ... @10:35:04.66 Intake.select :close @10:35:04.79 SSV.dial :exhaust @10:35:05.58 Exhaust.select :open @10:35:05.68 SS.reconfigure SSexhale @10:35:05.72 SS.seek 0.00ml @10:35:08.69 delay 3 seconds @10:35:11.70 AFV.select :holdingCoil @10:35:11.84 AS.reconfigure ASslow1 @10:35:11.87 AS.seek 0.489ml @10:35:14.69 Exhaust.select :close @10:35:14.79 SSV.dial CBV @10:35:14.81 CBV.dial SSV @10:35:15.62 Allowing 185s for 25.0ml of remaining 925.0ml @10:35:15.63 Intake.select :open @10:35:17.76 Intake @47.6psia, vacuum from 20.0 to 24.0psi @10:35:17.78 SS.reconfigure forSampling @10:35:17.81 SS.seek 25.00ml @10:35:20.33 Vacuum too high -- waiting up to 40s @10:35:20.59 Intake @46.0psia, vacuum from 20.0 to 24.0psi @10:35:20.61 SS.reconfigure forSampling @10:35:20.64 SS.seek 25.00ml @10:35:34.62 Vacuum too high -- waiting up to 40s @10:35:34.89 Intake @46.5psia, vacuum from 20.0 to 24.0psi @10:35:34.91 SS.reconfigure forSampling @10:35:34.94 SS.seek 25.00ml @10:36:07.21 delay 5 seconds @10:36:09.92 Stroke completed -- waiting for pressure to equalize ... @10:36:11.15 Intake.select :close @10:36:11.28 SSV.dial :exhaust @10:36:12.06 Exhaust.select :open @10:36:12.16 SS.reconfigure SSexhale @10:36:12.20 SS.seek 0.00ml @10:36:12.28 AS.reconfigure ASslow2 @10:36:12.89 AS.seek 0.454ml @10:36:21.02 Exhaust.select :close @10:36:21.12 SSV.dial CBV @10:36:21.14 CBV.dial SSV @10:36:21.94 Allowing 185s for 25.0ml of remaining 900.0ml @10:36:21.96 Intake.select :open @10:36:24.09 Intake @47.5psia, vacuum from 20.0 to 24.0psi @10:36:24.11 SS.reconfigure forSampling @10:36:24.14 SS.seek 25.00ml @10:36:33.04 Vacuum too high -- waiting up to 40s @10:36:33.30 Intake @46.8psia, vacuum from 20.0 to 24.0psi @10:36:33.32 SS.reconfigure forSampling @10:36:33.35 SS.seek 25.00ml @10:36:48.82 delay 1:00 @10:37:48.82 pushing eluate to holding coil @10:37:48.87 AS.seek 0.294ml @10:38:07.53 Stroke completed -- waiting for pressure to equalize ... @10:38:08.76 Intake.select :close @10:38:08.90 SSV.dial :exhaust @10:38:09.68 Exhaust.select :open @10:38:09.78 SS.reconfigure SSexhale @10:38:09.82 SS.seek 0.00ml @10:38:18.92 Exhaust.select :close @10:38:19.02 SSV.dial CBV @10:38:19.04 CBV.dial SSV @10:38:19.84 Allowing 185s for 25.0ml of remaining 875.0ml @10:38:19.86 Intake.select :open @10:38:21.99 Intake @47.0psia, vacuum from 20.0 to 24.0psi @10:38:22.01 SS.reconfigure forSampling @10:38:22.04 SS.seek 25.00ml @10:40:04.95 Vacuum too high -- waiting up to 40s @10:40:05.45 Intake @46.8psia, vacuum from 20.0 to 24.0psi @10:40:05.46 SS.reconfigure forSampling @10:40:05.49 SS.seek 25.00ml @10:40:13.00 Stroke completed -- waiting for pressure to equalize ... @10:40:15.39 Intake.select :close @10:40:15.52 SSV.dial :exhaust @10:40:16.33 Exhaust.select :open @10:40:16.43 SS.reconfigure SSexhale @10:40:16.47 SS.seek 0.00ml @10:40:25.64 Exhaust.select :close @10:40:25.74 SSV.dial CBV @10:40:25.76 CBV.dial SSV @10:40:26.58 Allowing 185s for 25.0ml of remaining 850.0ml @10:40:26.59 Intake.select :open @10:40:28.73 Intake @48.3psia, vacuum from 20.0 to 24.0psi @10:40:28.75 SS.reconfigure forSampling @10:40:28.78 SS.seek 25.00ml @10:40:32.41 delay 30 seconds @10:40:32.88 Vacuum too high -- waiting up to 40s @10:40:33.36 Intake @46.9psia, vacuum from 20.0 to 24.0psi @10:40:33.37 SS.reconfigure forSampling @10:40:33.41 SS.seek 25.00ml @10:40:38.87 Vacuum too high -- waiting up to 40s @10:40:39.35 Intake @47.3psia, vacuum from 20.0 to 24.0psi @10:40:39.36 SS.reconfigure forSampling @10:40:39.40 SS.seek 25.00ml @10:40:43.93 Vacuum too high -- waiting up to 40s @10:40:44.41 Intake @47.4psia, vacuum from 20.0 to 24.0psi @10:40:44.42 SS.reconfigure forSampling @10:40:44.46 SS.seek 25.00ml @10:40:47.44 Vacuum too high -- waiting up to 40s @10:40:47.93 Intake @47.0psia, vacuum from 20.0 to 24.0psi @10:40:47.94 SS.reconfigure forSampling @10:40:47.98 SS.seek 25.00ml @10:40:51.27 Vacuum too high -- waiting up to 40s @10:40:51.75 Intake @47.0psia, vacuum from 20.0 to 24.0psi @10:40:51.76 SS.reconfigure forSampling @10:40:51.80 SS.seek 25.00ml @10:40:55.71 Vacuum too high -- waiting up to 40s @10:40:56.19 Intake @47.7psia, vacuum from 20.0 to 24.0psi @10:40:56.20 SS.reconfigure forSampling @10:40:56.24 SS.seek 25.00ml @10:40:58.75 Vacuum too high -- waiting up to 40s @10:40:59.24 Intake @47.3psia, vacuum from 20.0 to 24.0psi @10:40:59.25 SS.reconfigure forSampling @10:40:59.29 SS.seek 25.00ml @10:41:01.88 Vacuum too high -- waiting up to 40s @10:41:02.37 Intake @46.9psia, vacuum from 20.0 to 24.0psi @10:41:02.39 SS.reconfigure forSampling @10:41:02.42 SS.seek 25.00ml @10:41:02.46 ATV.dial :waste,via => air3 @10:41:03.97 AS.reconfigure ASconfig @10:41:04.00 AS.seek empty @10:41:05.41 Vacuum too high -- waiting up to 40s @10:41:05.90 Intake @47.9psia, vacuum from 20.0 to 24.0psi @10:41:05.92 SS.reconfigure forSampling @10:41:05.95 SS.seek 25.00ml @10:41:08.06 Vacuum too high -- waiting up to 40s @10:41:08.55 Intake @47.4psia, vacuum from 20.0 to 24.0psi @10:41:08.57 SS.reconfigure forSampling @10:41:08.60 SS.seek 25.00ml @10:41:10.97 Vacuum too high -- waiting up to 40s @10:41:11.47 Intake @47.1psia, vacuum from 20.0 to 24.0psi @10:41:11.49 SS.reconfigure forSampling @10:41:11.52 SS.seek 25.00ml @10:41:13.91 Vacuum too high -- waiting up to 40s @10:41:14.40 Intake @47.2psia, vacuum from 20.0 to 24.0psi @10:41:14.42 SS.reconfigure forSampling @10:41:14.45 SS.seek 25.00ml @10:41:17.12 Vacuum too high -- waiting up to 40s @10:41:17.62 Intake @47.4psia, vacuum from 20.0 to 24.0psi @10:41:17.63 SS.reconfigure forSampling @10:41:17.66 SS.seek 25.00ml @10:41:19.94 Vacuum too high -- waiting up to 40s @10:41:20.43 Intake @47.3psia, vacuum from 20.0 to 24.0psi @10:41:20.45 SS.reconfigure forSampling @10:41:20.48 SS.seek 25.00ml @10:41:22.06 Parking Bleach in LysateSource line and SPEcolumn @10:41:22.07 ATV.dial :air3 @10:41:22.65 AS.reconfigure ASslow1 @10:41:22.71 AS.seek 0.029ml @10:41:22.73 Vacuum too high -- waiting up to 40s @10:41:23.22 Intake @47.3psia, vacuum from 20.0 to 24.0psi @10:41:23.24 SS.reconfigure forSampling @10:41:23.27 SS.seek 25.00ml @10:41:25.66 Vacuum too high -- waiting up to 40s @10:41:26.15 Intake @47.5psia, vacuum from 20.0 to 24.0psi @10:41:26.17 SS.reconfigure forSampling @10:41:26.20 SS.seek 25.00ml @10:41:28.25 Vacuum too high -- waiting up to 40s @10:41:28.74 Intake @47.0psia, vacuum from 20.0 to 24.0psi @10:41:28.76 SS.reconfigure forSampling @10:41:28.79 SS.seek 25.00ml @10:41:29.24 ATV.dial :ElutionWater @10:41:29.65 AS.seek 0.059ml @10:41:31.12 Vacuum too high -- waiting up to 40s @10:41:31.61 Intake @47.6psia, vacuum from 20.0 to 24.0psi @10:41:31.63 SS.reconfigure forSampling @10:41:31.66 SS.seek 25.00ml @10:41:33.59 Vacuum too high -- waiting up to 40s @10:41:34.07 Intake @47.3psia, vacuum from 20.0 to 24.0psi @10:41:34.08 SS.reconfigure forSampling @10:41:34.12 SS.seek 25.00ml @10:41:36.11 ATV.dial :air3 @10:41:36.25 Vacuum too high -- waiting up to 40s @10:41:36.55 AS.seek 0.069ml @10:41:36.81 Intake @47.7psia, vacuum from 20.0 to 24.0psi @10:41:36.83 SS.reconfigure forSampling @10:41:36.86 SS.seek 25.00ml @10:41:38.88 Vacuum too high -- waiting up to 40s @10:41:38.90 delay 2 seconds @10:41:39.36 Intake @47.2psia, vacuum from 20.0 to 24.0psi @10:41:39.38 SS.reconfigure forSampling @10:41:39.41 SS.seek 25.00ml @10:41:40.91 ATV.dial :bleach @10:41:41.30 AS.seek 0.709ml @10:41:41.59 Vacuum too high -- waiting up to 40s @10:41:42.08 Intake @47.3psia, vacuum from 20.0 to 24.0psi @10:41:42.10 SS.reconfigure forSampling @10:41:42.13 SS.seek 25.00ml @10:41:44.06 Vacuum too high -- waiting up to 40s @10:41:44.75 Intake @48.3psia, vacuum from 20.0 to 24.0psi @10:41:44.77 SS.reconfigure forSampling @10:41:44.80 SS.seek 25.00ml @10:41:46.84 Vacuum too high -- waiting up to 40s @10:41:47.98 Intake @47.4psia, vacuum from 20.0 to 24.0psi @10:41:48.00 SS.reconfigure forSampling @10:41:48.03 SS.seek 25.00ml @10:41:50.59 Vacuum too high -- waiting up to 40s @10:41:51.08 Intake @47.3psia, vacuum from 20.0 to 24.0psi @10:41:51.10 SS.reconfigure forSampling @10:41:51.13 SS.seek 25.00ml @10:41:53.69 Vacuum too high -- waiting up to 40s @10:41:54.41 Intake @48.0psia, vacuum from 20.0 to 24.0psi @10:41:54.43 SS.reconfigure forSampling @10:41:54.46 SS.seek 25.00ml @10:41:56.44 Vacuum too high -- waiting up to 40s @10:41:56.93 Intake @47.1psia, vacuum from 20.0 to 24.0psi @10:41:56.95 SS.reconfigure forSampling @10:41:56.98 SS.seek 25.00ml @10:42:00.15 Vacuum too high -- waiting up to 40s @10:42:00.87 Intake @46.8psia, vacuum from 20.0 to 24.0psi @10:42:00.88 SS.reconfigure forSampling @10:42:00.91 SS.seek 25.00ml @10:42:03.37 Vacuum too high -- waiting up to 40s @10:42:04.08 Intake @47.7psia, vacuum from 20.0 to 24.0psi @10:42:04.10 SS.reconfigure forSampling @10:42:04.13 SS.seek 25.00ml @10:42:06.22 Vacuum too high -- waiting up to 40s @10:42:06.93 Intake @47.9psia, vacuum from 20.0 to 24.0psi @10:42:06.95 SS.reconfigure forSampling @10:42:06.98 SS.seek 25.00ml @10:42:08.81 Vacuum too high -- waiting up to 40s @10:42:09.30 Intake @47.6psia, vacuum from 20.0 to 24.0psi @10:42:09.32 SS.reconfigure forSampling @10:42:09.35 SS.seek 25.00ml @10:42:11.50 Vacuum too high -- waiting up to 40s @10:42:12.21 Intake @47.1psia, vacuum from 20.0 to 24.0psi @10:42:12.23 SS.reconfigure forSampling @10:42:12.26 SS.seek 25.00ml @10:42:14.53 Vacuum too high -- waiting up to 40s @10:42:15.24 Intake @46.8psia, vacuum from 20.0 to 24.0psi @10:42:15.25 SS.reconfigure forSampling @10:42:15.29 SS.seek 25.00ml @10:42:17.87 Vacuum too high -- waiting up to 40s @10:42:18.57 Intake @48.9psia, vacuum from 20.0 to 24.0psi @10:42:18.58 SS.reconfigure forSampling @10:42:18.62 SS.seek 25.00ml @10:42:20.03 Vacuum too high -- waiting up to 40s @10:42:20.53 Intake @47.0psia, vacuum from 20.0 to 24.0psi @10:42:20.55 SS.reconfigure forSampling @10:42:20.58 SS.seek 25.00ml @10:42:22.78 Vacuum too high -- waiting up to 40s @10:42:23.48 Intake @46.7psia, vacuum from 20.0 to 24.0psi @10:42:23.49 SS.reconfigure forSampling @10:42:23.53 SS.seek 25.00ml @10:42:26.06 Vacuum too high -- waiting up to 40s @10:42:26.76 Intake @47.5psia, vacuum from 20.0 to 24.0psi @10:42:26.78 SS.reconfigure forSampling @10:42:26.81 SS.seek 25.00ml @10:42:28.84 Vacuum too high -- waiting up to 40s @10:42:29.54 Intake @48.0psia, vacuum from 20.0 to 24.0psi @10:42:29.55 SS.reconfigure forSampling @10:42:29.59 SS.seek 25.00ml @10:42:31.03 Vacuum too high -- waiting up to 40s @10:42:31.52 Intake @47.1psia, vacuum from 20.0 to 24.0psi @10:42:31.53 SS.reconfigure forSampling @10:42:31.57 SS.seek 25.00ml @10:42:33.40 Vacuum too high -- waiting up to 40s @10:42:34.33 Intake @47.3psia, vacuum from 20.0 to 24.0psi @10:42:34.37 SS.reconfigure forSampling @10:42:34.44 SS.seek 25.00ml @10:42:36.90 Vacuum too high -- waiting up to 40s @10:42:37.62 Intake @47.5psia, vacuum from 20.0 to 24.0psi @10:42:37.63 SS.reconfigure forSampling @10:42:37.66 SS.seek 25.00ml @10:42:39.59 Vacuum too high -- waiting up to 40s @10:42:40.31 Intake @47.7psia, vacuum from 20.0 to 24.0psi @10:42:40.32 SS.reconfigure forSampling @10:42:40.35 SS.seek 25.00ml @10:42:42.06 Vacuum too high -- waiting up to 40s @10:42:42.55 Intake @47.2psia, vacuum from 20.0 to 24.0psi @10:42:42.57 SS.reconfigure forSampling @10:42:42.60 SS.seek 25.00ml @10:42:44.37 Vacuum too high -- waiting up to 40s @10:42:45.08 Intake @47.6psia, vacuum from 20.0 to 24.0psi @10:42:45.10 SS.reconfigure forSampling @10:42:45.13 SS.seek 25.00ml @10:42:46.87 Vacuum too high -- waiting up to 40s @10:42:47.58 Intake @47.8psia, vacuum from 20.0 to 24.0psi @10:42:47.60 SS.reconfigure forSampling @10:42:47.63 SS.seek 25.00ml @10:42:49.37 Vacuum too high -- waiting up to 40s @10:42:50.08 Intake @47.7psia, vacuum from 20.0 to 24.0psi @10:42:50.09 SS.reconfigure forSampling @10:42:50.13 SS.seek 25.00ml @10:42:51.81 Vacuum too high -- waiting up to 40s @10:42:52.52 Intake @47.5psia, vacuum from 20.0 to 24.0psi @10:42:52.54 SS.reconfigure forSampling @10:42:52.57 SS.seek 25.00ml @10:42:54.40 Vacuum too high -- waiting up to 40s @10:42:55.11 Intake @47.3psia, vacuum from 20.0 to 24.0psi @10:42:55.12 SS.reconfigure forSampling @10:42:55.16 SS.seek 25.00ml @10:42:57.06 Vacuum too high -- waiting up to 40s @10:42:57.78 SS.seek 25.00ml @10:42:58.68 Stroke completed -- waiting for pressure to equalize ... @10:43:01.09 Intake.select :close @10:43:01.22 SSV.dial :exhaust @10:43:02.02 Exhaust.select :open @10:43:02.12 SS.reconfigure SSexhale @10:43:02.16 SS.seek 0.00ml @10:43:11.22 Exhaust.select :close @10:43:11.32 SSV.dial CBV @10:43:11.34 CBV.dial SSV @10:43:12.14 Allowing 185s for 25.0ml of remaining 825.0ml @10:43:12.16 Intake.select :open @10:43:14.29 Intake @47.6psia, vacuum from 20.0 to 24.0psi @10:43:14.31 SS.reconfigure forSampling @10:43:14.34 SS.seek 25.00ml @10:43:16.74 Vacuum too high -- waiting up to 40s @10:43:17.23 Intake @47.1psia, vacuum from 20.0 to 24.0psi @10:43:17.24 SS.reconfigure forSampling @10:43:17.28 SS.seek 25.00ml @10:43:18.77 Vacuum too high -- waiting up to 40s @10:43:19.27 SS.seek 25.00ml @10:43:20.62 Vacuum too high -- waiting up to 40s @10:43:21.11 Intake @47.2psia, vacuum from 20.0 to 24.0psi @10:43:21.13 SS.reconfigure forSampling @10:43:21.16 SS.seek 25.00ml @10:43:22.56 Vacuum too high -- waiting up to 40s @10:43:23.95 Intake @48.0psia, vacuum from 20.0 to 24.0psi @10:43:23.96 SS.reconfigure forSampling @10:43:23.99 SS.seek 25.00ml @10:43:25.99 Vacuum too high -- waiting up to 40s @10:43:26.48 Intake @47.3psia, vacuum from 20.0 to 24.0psi @10:43:26.50 SS.reconfigure forSampling @10:43:26.53 SS.seek 25.00ml @10:43:28.02 Vacuum too high -- waiting up to 40s @10:43:28.73 Intake @47.3psia, vacuum from 20.0 to 24.0psi @10:43:28.75 SS.reconfigure forSampling @10:43:28.78 SS.seek 25.00ml @10:43:30.31 Vacuum too high -- waiting up to 40s @10:43:30.80 Intake @46.9psia, vacuum from 20.0 to 24.0psi @10:43:30.82 SS.reconfigure forSampling @10:43:30.85 SS.seek 25.00ml @10:43:32.34 Vacuum too high -- waiting up to 40s @10:43:33.05 Intake @47.6psia, vacuum from 20.0 to 24.0psi @10:43:33.07 SS.reconfigure forSampling @10:43:33.10 SS.seek 25.00ml @10:43:34.74 Vacuum too high -- waiting up to 40s @10:43:35.45 Intake @48.3psia, vacuum from 20.0 to 24.0psi @10:43:35.47 SS.reconfigure forSampling @10:43:35.51 SS.seek 25.00ml @10:43:36.90 Vacuum too high -- waiting up to 40s @10:43:37.39 Intake @47.8psia, vacuum from 20.0 to 24.0psi @10:43:37.41 SS.reconfigure forSampling @10:43:37.44 SS.seek 25.00ml @10:43:38.77 Vacuum too high -- waiting up to 40s @10:43:39.25 Intake @47.6psia, vacuum from 20.0 to 24.0psi @10:43:39.27 SS.reconfigure forSampling @10:43:39.30 SS.seek 25.00ml @10:43:40.62 Vacuum too high -- waiting up to 40s @10:43:41.11 Intake @47.6psia, vacuum from 20.0 to 24.0psi @10:43:41.13 SS.reconfigure forSampling @10:43:41.16 SS.seek 25.00ml @10:43:42.43 Vacuum too high -- waiting up to 40s @10:43:42.98 Intake @47.7psia, vacuum from 20.0 to 24.0psi @10:43:43.00 SS.reconfigure forSampling @10:43:43.03 SS.seek 25.00ml @10:43:44.43 Vacuum too high -- waiting up to 40s @10:43:44.91 Intake @47.4psia, vacuum from 20.0 to 24.0psi @10:43:44.93 SS.reconfigure forSampling @10:43:44.96 SS.seek 25.00ml @10:43:46.30 Vacuum too high -- waiting up to 40s @10:43:47.00 Intake @47.8psia, vacuum from 20.0 to 24.0psi @10:43:47.02 SS.reconfigure forSampling @10:43:47.05 SS.seek 25.00ml @10:43:48.55 Vacuum too high -- waiting up to 40s @10:43:49.26 Intake @48.6psia, vacuum from 20.0 to 24.0psi @10:43:49.28 SS.reconfigure forSampling @10:43:49.31 SS.seek 25.00ml @10:43:50.74 Vacuum too high -- waiting up to 40s @10:43:51.23 Intake @47.4psia, vacuum from 20.0 to 24.0psi @10:43:51.24 SS.reconfigure forSampling @10:43:51.28 SS.seek 25.00ml @10:43:52.07 delay 3 seconds @10:43:52.71 Vacuum too high -- waiting up to 40s @10:43:53.42 SS.seek 25.00ml @10:43:54.93 Vacuum too high -- waiting up to 40s @10:43:55.08 ATV.dial :air3 @10:43:55.47 Intake @47.3psia, vacuum from 20.0 to 24.0psi @10:43:55.48 SS.reconfigure forSampling @10:43:55.51 SS.seek 25.00ml @10:43:55.59 delay 3 seconds @10:43:57.02 Vacuum too high -- waiting up to 40s @10:43:57.73 Intake @47.7psia, vacuum from 20.0 to 24.0psi @10:43:58.41 SS.reconfigure forSampling @10:43:58.44 SS.seek 25.00ml @10:43:58.64 AS.seek 0.734ml @10:44:00.64 Vacuum too high -- waiting up to 40s @10:44:01.14 Intake @47.5psia, vacuum from 20.0 to 24.0psi @10:44:01.15 SS.reconfigure forSampling @10:44:01.18 SS.seek 25.00ml @10:44:02.68 Vacuum too high -- waiting up to 40s @10:44:03.17 Intake @47.6psia, vacuum from 20.0 to 24.0psi @10:44:03.19 SS.reconfigure forSampling @10:44:03.22 SS.seek 25.00ml @10:44:03.97 delay 3 seconds @10:44:04.43 Vacuum too high -- waiting up to 40s @10:44:04.92 Intake @47.1psia, vacuum from 20.0 to 24.0psi @10:44:04.94 SS.reconfigure forSampling @10:44:04.97 SS.seek 25.00ml @10:44:06.40 Vacuum too high -- waiting up to 40s @10:44:06.99 ATV.dial :bleach @10:44:07.16 Intake @47.6psia, vacuum from 20.0 to 24.0psi @10:44:07.17 SS.reconfigure forSampling @10:44:07.20 SS.seek 25.00ml @10:44:07.44 AS.seek 0.719ml @10:44:08.68 Vacuum too high -- waiting up to 40s @10:44:09.17 Intake @47.7psia, vacuum from 20.0 to 24.0psi @10:44:09.18 SS.reconfigure forSampling @10:44:09.22 SS.seek 25.00ml @10:44:10.40 Vacuum too high -- waiting up to 40s @10:44:10.76 delay 3 seconds @10:44:11.13 Intake @47.9psia, vacuum from 20.0 to 24.0psi @10:44:11.14 SS.reconfigure forSampling @10:44:11.18 SS.seek 25.00ml @10:44:12.58 Vacuum too high -- waiting up to 40s @10:44:13.07 Intake @47.9psia, vacuum from 20.0 to 24.0psi @10:44:13.09 SS.reconfigure forSampling @10:44:13.12 SS.seek 25.00ml @10:44:13.77 ATV.dial :AIV @10:44:14.33 Vacuum too high -- waiting up to 40s @10:44:14.81 Intake @47.6psia, vacuum from 20.0 to 24.0psi @10:44:14.83 SS.reconfigure forSampling @10:44:14.86 SS.seek 25.00ml @10:44:14.91 AS.reconfigure ASconfig @10:44:14.95 AS.seek 0.015ml @10:44:16.05 Vacuum too high -- waiting up to 40s @10:44:16.76 Intake @47.8psia, vacuum from 20.0 to 24.0psi @10:44:16.78 SS.reconfigure forSampling @10:44:16.81 SS.seek 25.00ml @10:44:18.27 Vacuum too high -- waiting up to 40s @10:44:18.99 SS.seek 25.00ml @10:44:20.46 Vacuum too high -- waiting up to 40s @10:44:20.95 Intake @47.4psia, vacuum from 20.0 to 24.0psi @10:44:20.97 SS.reconfigure forSampling @10:44:21.00 SS.seek 25.00ml @10:44:22.18 Vacuum too high -- waiting up to 40s @10:44:22.67 Intake @47.3psia, vacuum from 20.0 to 24.0psi @10:44:22.69 SS.reconfigure forSampling @10:44:22.72 SS.seek 25.00ml @10:44:23.83 Vacuum too high -- waiting up to 40s @10:44:24.54 Intake @47.8psia, vacuum from 20.0 to 24.0psi @10:44:24.56 SS.reconfigure forSampling @10:44:24.59 SS.seek 25.00ml @10:44:25.90 Vacuum too high -- waiting up to 40s @10:44:26.39 Intake @47.9psia, vacuum from 20.0 to 24.0psi @10:44:26.41 SS.reconfigure forSampling @10:44:26.44 SS.seek 25.00ml @10:44:27.68 Vacuum too high -- waiting up to 40s @10:44:28.17 Intake @47.8psia, vacuum from 20.0 to 24.0psi @10:44:28.19 SS.reconfigure forSampling @10:44:28.22 SS.seek 25.00ml @10:44:29.33 Vacuum too high -- waiting up to 40s @10:44:30.24 Intake @47.4psia, vacuum from 20.0 to 24.0psi @10:44:30.25 SS.reconfigure forSampling @10:44:30.29 SS.seek 25.00ml @10:44:32.02 Vacuum too high -- waiting up to 40s @10:44:32.74 Intake @48.2psia, vacuum from 20.0 to 24.0psi @10:44:32.75 SS.reconfigure forSampling @10:44:32.78 SS.seek 25.00ml @10:44:34.08 Vacuum too high -- waiting up to 40s @10:44:34.57 Intake @47.6psia, vacuum from 20.0 to 24.0psi @10:44:34.59 SS.reconfigure forSampling @10:44:34.62 SS.seek 25.00ml @10:44:35.77 Vacuum too high -- waiting up to 40s @10:44:36.48 Intake @47.4psia, vacuum from 20.0 to 24.0psi @10:44:36.50 SS.reconfigure forSampling @10:44:36.53 SS.seek 25.00ml @10:44:37.83 Vacuum too high -- waiting up to 40s @10:44:38.54 Intake @47.3psia, vacuum from 20.0 to 24.0psi @10:44:38.56 SS.reconfigure forSampling @10:44:38.59 SS.seek 25.00ml @10:44:40.05 Vacuum too high -- waiting up to 40s @10:44:40.75 Intake @47.9psia, vacuum from 20.0 to 24.0psi @10:44:40.77 SS.reconfigure forSampling @10:44:40.80 SS.seek 25.00ml @10:44:42.08 Vacuum too high -- waiting up to 40s @10:44:42.79 Intake @47.8psia, vacuum from 20.0 to 24.0psi @10:44:42.81 SS.reconfigure forSampling @10:44:42.84 SS.seek 25.00ml @10:44:44.15 Vacuum too high -- waiting up to 40s @10:44:44.86 Intake @47.9psia, vacuum from 20.0 to 24.0psi @10:44:44.88 SS.reconfigure forSampling @10:44:44.91 SS.seek 25.00ml @10:44:46.21 Vacuum too high -- waiting up to 40s @10:44:46.92 SS.seek 25.00ml @10:44:48.27 Vacuum too high -- waiting up to 40s @10:44:48.97 Intake @47.4psia, vacuum from 20.0 to 24.0psi @10:44:48.98 SS.reconfigure forSampling @10:44:49.02 SS.seek 25.00ml @10:44:50.43 Vacuum too high -- waiting up to 40s @10:44:51.14 Intake @47.2psia, vacuum from 20.0 to 24.0psi @10:44:51.16 SS.reconfigure forSampling @10:44:51.19 SS.seek 25.00ml @10:44:52.96 Vacuum too high -- waiting up to 40s @10:44:53.88 Intake @47.3psia, vacuum from 20.0 to 24.0psi @10:44:53.90 SS.reconfigure forSampling @10:44:53.93 SS.seek 25.00ml @10:44:55.74 Vacuum too high -- waiting up to 40s @10:44:56.28 ATV.dial :waste @10:44:56.66 Intake @48.3psia, vacuum from 20.0 to 24.0psi @10:44:56.67 SS.reconfigure forSampling @10:44:56.71 SS.seek 25.00ml @10:44:57.61 AS.seek empty @10:44:58.11 Vacuum too high -- waiting up to 40s @10:44:58.83 ATV.dial :air3 @10:44:58.87 Intake @47.8psia, vacuum from 20.0 to 24.0psi @10:44:58.89 SS.reconfigure forSampling @10:44:58.92 SS.seek 25.00ml @10:44:59.42 AS.reconfigure ASslow1 @10:44:59.46 AS.seek 0.009ml @10:45:00.34 Vacuum too high -- waiting up to 40s @10:45:01.74 Intake @47.6psia, vacuum from 20.0 to 24.0psi @10:45:01.76 SS.reconfigure forSampling @10:45:01.80 SS.seek 25.00ml @10:45:01.84 delay 2 seconds @10:45:03.85 ATV.dial :bleach @10:45:03.90 Vacuum too high -- waiting up to 40s @10:45:04.28 AS.seek 0.579ml @10:45:04.45 Intake @47.2psia, vacuum from 20.0 to 24.0psi @10:45:04.46 SS.reconfigure forSampling @10:45:04.50 SS.seek 25.00ml @10:45:06.11 Vacuum too high -- waiting up to 40s @10:45:07.04 Intake @46.9psia, vacuum from 20.0 to 24.0psi @10:45:07.06 SS.reconfigure forSampling @10:45:07.09 SS.seek 25.00ml @10:45:08.77 Vacuum too high -- waiting up to 40s @10:45:09.70 Intake @47.7psia, vacuum from 20.0 to 24.0psi @10:45:09.72 SS.reconfigure forSampling @10:45:09.75 SS.seek 25.00ml @10:45:11.30 Vacuum too high -- waiting up to 40s @10:45:12.01 Intake @48.2psia, vacuum from 20.0 to 24.0psi @10:45:12.03 SS.reconfigure forSampling @10:45:12.06 SS.seek 25.00ml @10:45:13.14 Vacuum too high -- waiting up to 40s @10:45:13.85 Intake @47.4psia, vacuum from 20.0 to 24.0psi @10:45:13.87 SS.reconfigure forSampling @10:45:13.90 SS.seek 25.00ml @10:45:15.61 Vacuum too high -- waiting up to 40s @10:45:16.54 Intake @47.5psia, vacuum from 20.0 to 24.0psi @10:45:16.56 SS.reconfigure forSampling @10:45:16.59 SS.seek 25.00ml @10:45:18.08 Vacuum too high -- waiting up to 40s @10:45:19.00 Intake @47.4psia, vacuum from 20.0 to 24.0psi @10:45:19.01 SS.reconfigure forSampling @10:45:19.05 SS.seek 25.00ml @10:45:20.77 Vacuum too high -- waiting up to 40s @10:45:21.70 Intake @47.8psia, vacuum from 20.0 to 24.0psi @10:45:21.72 SS.reconfigure forSampling @10:45:21.75 SS.seek 25.00ml @10:45:23.17 Vacuum too high -- waiting up to 40s @10:45:23.88 Intake @47.3psia, vacuum from 20.0 to 24.0psi @10:45:23.90 SS.reconfigure forSampling @10:45:23.93 SS.seek 25.00ml @10:45:25.39 Vacuum too high -- waiting up to 40s @10:45:26.31 Intake @47.8psia, vacuum from 20.0 to 24.0psi @10:45:26.32 SS.reconfigure forSampling @10:45:26.36 SS.seek 25.00ml @10:45:28.14 Vacuum too high -- waiting up to 40s @10:45:29.06 Intake @48.0psia, vacuum from 20.0 to 24.0psi @10:45:29.08 SS.reconfigure forSampling @10:45:29.11 SS.seek 25.00ml @10:45:30.55 Vacuum too high -- waiting up to 40s @10:45:31.26 Intake @47.6psia, vacuum from 20.0 to 24.0psi @10:45:31.28 SS.reconfigure forSampling @10:45:31.31 SS.seek 25.00ml @10:45:32.80 Vacuum too high -- waiting up to 40s @10:45:33.72 Intake @47.0psia, vacuum from 20.0 to 24.0psi @10:45:33.74 SS.reconfigure forSampling @10:45:34.41 SS.seek 25.00ml @10:45:36.77 Vacuum too high -- waiting up to 40s @10:45:37.70 Intake @48.0psia, vacuum from 20.0 to 24.0psi @10:45:37.72 SS.reconfigure forSampling @10:45:37.75 SS.seek 25.00ml @10:45:39.39 Vacuum too high -- waiting up to 40s @10:45:40.10 Intake @47.5psia, vacuum from 20.0 to 24.0psi @10:45:40.12 SS.reconfigure forSampling @10:45:40.15 SS.seek 25.00ml @10:45:41.70 Vacuum too high -- waiting up to 40s @10:45:42.63 Intake @47.6psia, vacuum from 20.0 to 24.0psi @10:45:42.65 SS.reconfigure forSampling @10:45:42.68 SS.seek 25.00ml @10:45:44.11 Vacuum too high -- waiting up to 40s @10:45:45.04 Intake @47.7psia, vacuum from 20.0 to 24.0psi @10:45:45.06 SS.reconfigure forSampling @10:45:45.09 SS.seek 25.00ml @10:45:46.80 Vacuum too high -- waiting up to 40s @10:45:47.73 Intake @47.9psia, vacuum from 20.0 to 24.0psi @10:45:47.75 SS.reconfigure forSampling @10:45:47.78 SS.seek 25.00ml @10:45:49.36 Vacuum too high -- waiting up to 40s @10:45:50.06 Intake @47.9psia, vacuum from 20.0 to 24.0psi @10:45:50.08 SS.reconfigure forSampling @10:45:50.11 SS.seek 25.00ml @10:45:51.30 Vacuum too high -- waiting up to 40s @10:45:52.00 Intake @47.6psia, vacuum from 20.0 to 24.0psi @10:45:52.02 SS.reconfigure forSampling @10:45:52.05 SS.seek 25.00ml @10:45:53.52 Vacuum too high -- waiting up to 40s @10:45:54.44 Intake @47.3psia, vacuum from 20.0 to 24.0psi @10:45:54.46 SS.reconfigure forSampling @10:45:54.49 SS.seek 25.00ml @10:45:56.11 Vacuum too high -- waiting up to 40s @10:45:57.03 Intake @47.9psia, vacuum from 20.0 to 24.0psi @10:45:57.04 SS.reconfigure forSampling @10:45:57.08 SS.seek 25.00ml @10:45:58.48 Vacuum too high -- waiting up to 40s @10:45:59.25 Intake @48.2psia, vacuum from 20.0 to 24.0psi @10:45:59.27 SS.reconfigure forSampling @10:45:59.30 SS.seek 25.00ml @10:46:00.36 Vacuum too high -- waiting up to 40s @10:46:01.04 Intake @47.7psia, vacuum from 20.0 to 24.0psi @10:46:01.05 SS.reconfigure forSampling @10:46:01.09 SS.seek 25.00ml @10:46:02.67 Vacuum too high -- waiting up to 40s @10:46:03.37 Intake @47.3psia, vacuum from 20.0 to 24.0psi @10:46:03.39 SS.reconfigure forSampling @10:46:03.42 SS.seek 25.00ml @10:46:04.77 Vacuum too high -- waiting up to 40s @10:46:05.68 Intake @47.4psia, vacuum from 20.0 to 24.0psi @10:46:05.70 SS.reconfigure forSampling @10:46:05.73 SS.seek 25.00ml @10:46:07.52 Vacuum too high -- waiting up to 40s @10:46:09.14 Intake @48.2psia, vacuum from 20.0 to 24.0psi @10:46:09.16 SS.reconfigure forSampling @10:46:09.19 SS.seek 25.00ml @10:46:11.08 Vacuum too high -- waiting up to 40s @10:46:11.79 Intake @47.5psia, vacuum from 20.0 to 24.0psi @10:46:11.81 SS.reconfigure forSampling @10:46:11.84 SS.seek 25.00ml @10:46:13.27 Vacuum too high -- waiting up to 40s @10:46:13.99 SS.seek 25.00ml @10:46:15.34 Vacuum too high -- waiting up to 40s @10:46:16.06 Intake @47.6psia, vacuum from 20.0 to 24.0psi @10:46:16.08 SS.reconfigure forSampling @10:46:16.11 SS.seek 25.00ml @10:46:17.17 Vacuum too high -- waiting up to 40s @10:46:17.19 SS.reconfigure SSconfig @10:46:17.22 SS.hold @10:46:17.32 Intake.select :close @10:46:17.47 ShallowSampler::Clogged in WCR -- progress too slow after 198.9ml @10:46:17.48 SSV.dial :exhaust @10:46:18.28 Exhaust.select :open @10:46:18.38 SS.reconfigure SSexhale @10:46:18.42 SS.seek 0.00ml @10:46:26.95 Exhaust.select :close @10:46:27.06 Sampled 198.9ml @10:46:27.07 collectSample: Complete @10:46:27.08 Drying puck before fixing for 04:00.00 @10:46:27.09 CBV.dial :waste2b @10:46:27.88 delay 1:00 @10:46:30.05 Sleepy.power :sampler,:OFF @10:47:00.80 delay 3 seconds @10:47:03.81 ATV.dial :air3 @10:47:04.15 delay 3 seconds @10:47:07.21 AS.seek 0.604ml @10:47:12.57 delay 3 seconds @10:47:15.58 ATV.dial :bleach @10:47:16.07 AS.seek 0.589ml @10:47:19.36 delay 3 seconds @10:47:22.37 ATV.dial :ASV @10:47:23.26 ASV.dial :wasteR @10:47:24.12 AS.seek 0.000ml @10:47:27.88 CSV.dial :air @10:47:28.31 CS.reconfigure CSfast @10:47:28.34 CS.seek 10.000ml @10:47:52.22 CBV.dial between CSV and waste2b @10:47:52.53 Pumping wcr puck from tube 2 up to 55psi... @10:47:52.58 CBV.dial SSV @10:47:54.00 CBV.dial between CSV and waste2b @10:47:54.93 CSV.dial between air and CTV @10:47:55.93 CS.seek 7.035ml @10:48:03.54 CTV.dial CSV,avoiding => air @10:48:03.56 CSV.dial CTV @10:48:04.31 CS.reconfigure CSpressurize @10:48:04.34 CS.seek 0.000ml @10:48:19.71 CBV.dial :waste2b @10:48:20.03 CS.reconfigure CSconfig @10:48:20.06 CS.seek empty @10:48:23.66 delay 1:00 @10:49:23.67 CTV.dial :air,avoiding => intake @10:49:24.58 delay 1:00 @10:49:24.95 Fix #1 of 2 (with rnal) for 02:00.00 @10:49:24.96 CS Pulling 2ml of rnal CSV.dial CSR @10:49:25.39 CSR.select :rnal @10:49:25.49 CS.seek 2.000ml @10:49:31.40 delay 5 seconds @10:49:36.41 CSR.select :air @10:49:36.50 CS.reconfigure CSfast @10:49:36.54 CS.seek 10.000ml @10:49:55.59 CBV.dial between CSV and waste2b @10:49:55.91 Pumping wcr puck from tube 2 up to 55psi... @10:49:55.95 CBV.dial SSV @10:49:57.36 CBV.dial between CSV and waste2b @10:49:58.30 CSV.dial between air and CTV @10:49:58.96 CS.seek 6.972ml @10:50:06.76 CTV.dial CSV,avoiding => air @10:50:06.78 CSV.dial CTV @10:50:08.13 CS.reconfigure CSpressurize @10:50:08.16 CS.seek 0.000ml @10:50:24.59 AFV.select :carrierSolution @10:50:24.69 delay 2 seconds @10:50:25.24 delay 2:00 @10:50:26.70 AS.reconfigure ASconfig @10:50:26.74 AS.seek 1.000ml @10:51:25.42 delay 1 second @10:51:26.43 AFV.select :holdingCoil @10:51:26.52 delay 1 second @10:51:27.53 ATV.dial :ASV @10:51:27.54 ASV.dial :wasteR @10:51:28.16 AS.reconfigure ASslow1 @10:51:28.19 AS.seek 0.000ml @10:52:25.25 CBV.dial :waste2b @10:52:25.58 CS.reconfigure CSconfig @10:52:25.61 CS.seek empty @10:52:27.33 delay 1:00 @10:53:27.34 CTV.dial :air,avoiding => intake @10:53:28.61 Fix #2 of 2 (with rnal) for 02:00.00 @10:53:28.62 CS Pulling 2ml of rnal CSV.dial CSR @10:53:29.13 CSR.select :rnal @10:53:29.23 CS.seek 2.000ml @10:53:35.21 delay 5 seconds @10:53:40.22 CSR.select :air @10:53:40.31 CS.reconfigure CSfast @10:53:40.35 CS.seek 10.000ml @10:53:59.52 CBV.dial between CSV and waste2b @10:53:59.85 Pumping wcr puck from tube 2 up to 55psi... @10:53:59.90 CBV.dial SSV @10:54:01.31 CBV.dial between CSV and waste2b @10:54:02.24 CSV.dial between air and CTV @10:54:02.90 CS.seek 6.886ml @10:54:10.81 CTV.dial CSV,avoiding => air @10:54:10.83 CSV.dial CTV @10:54:12.20 CS.reconfigure CSpressurize @10:54:12.23 CS.seek 0.000ml @10:54:28.97 delay 2:00 @10:54:52.43 delay 20 seconds @10:55:12.44 ATV.dial :air3 @10:55:13.65 AS.seek 0.590ml @10:56:28.98 CBV.dial :waste2b @10:56:29.30 CS.reconfigure CSconfig @10:56:29.33 CS.seek empty @10:56:31.17 delay 1:00 @10:57:14.25 delay 3 seconds @10:57:17.27 ATV.dial :ASV @10:57:18.35 ASV.dial :wasteR @10:57:18.41 AS.seek 0.090ml @10:57:31.19 CTV.dial :air,avoiding => intake @10:57:32.50 Storing wcr puck in Collection Clamp to tube 1 @10:57:32.63 SC.reconfigure SCtight @10:57:32.66 SC.seek 1 @10:57:34.93 SC.reconfigure SCloose @10:57:35.11 Elbow.reconfigure TightElbow @10:57:35.14 Elbow.seek 1 @10:57:36.76 Forearm.reconfigure TightForearm @10:57:36.79 Forearm.seek 1 + 800 counts @10:57:37.90 SE.reconfigure SEpick @10:57:37.95 SE.seek beamBreak @10:57:39.39 SE.configure SEconfig @10:57:39.51 Elbow.reconfigure LimpElbow @10:57:39.55 Forearm.reconfigure LooseForearm @10:57:39.59 Forearm.seek 1 @10:57:39.81 Elbow.hold @10:57:39.86 Elbow.reconfigure TightElbow @10:57:39.94 SE.seek 16.05 Pucks below Top Plate @10:57:40.27 Forearm.coast @10:57:40.31 Forearm.seek 1 + 301 counts @10:57:40.55 Forearm.reconfigure TightForearm @10:57:40.58 Forearm.seek 1 + 800 counts @10:57:40.81 SE.seek 17.05 Pucks below Top Plate @10:57:41.37 Forearm.reconfigure LooseForearm @10:57:41.40 Forearm.seek retract @10:57:42.37 Elbow.seek CC @10:57:43.96 Elbow.reconfigure LooseElbow @10:57:44.04 Forearm.seek CC @10:57:45.00 CC.seek sealed @10:57:45.95 Hand.grip @10:57:46.64 CC.seek open @10:57:50.03 Forearm.seek retract @10:57:51.61 Elbow.reconfigure TightElbow @10:57:51.65 Elbow.seek 1 @10:57:53.32 Elbow.reconfigure LimpElbow @10:57:53.36 Forearm.seek 1 @10:57:54.40 Elbow.hold @10:57:54.45 Elbow.reconfigure TightElbow @10:57:54.48 Hand.release @10:57:55.34 Forearm.coast @10:57:55.38 Forearm.seek 1 + 314 counts @10:57:55.62 Forearm.reconfigure TightForearm @10:57:55.66 Forearm.seek 1 + 800 counts @10:57:55.88 SE.seek down @10:57:57.00 SE.coast @10:57:57.02 Forearm.reconfigure LooseForearm @10:57:57.07 Forearm.seek retract @10:57:57.99 SC.coast @10:57:58.02 Starting finalPurge @10:57:58.03 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @10:57:58.19 Elbow.seek FlushPuck::Garage @10:57:59.96 Elbow.reconfigure LooseElbow @10:58:00.04 Forearm.seek garage @10:58:01.07 Hand.grip @10:58:01.85 Forearm.seek retract @10:58:02.89 Elbow.reconfigure TightElbow @10:58:02.92 Elbow.seek CC @10:58:05.72 Elbow.reconfigure LooseElbow @10:58:05.80 Forearm.reconfigure TightForearm @10:58:05.83 Forearm.seek CC @10:58:06.94 CC.seek closed @10:58:10.73 Collection Clamp onSpring/offSpring for 5/22 samples, on/off torque 2.08 @10:58:10.78 Hand.release @10:58:11.55 Forearm.reconfigure LooseForearm @10:58:11.58 Forearm.seek retract @10:58:12.55 Flushing the Collection Valve::Manifold @10:58:12.65 CS Pulling 5ml of flush @10:58:12.66 CSV.dial CSR @10:58:13.15 CSR.select :flush @10:58:13.25 CS.seek 5.000ml @10:58:26.76 delay 5 seconds @10:58:31.77 CSR.select :air @10:58:31.91 CS.reconfigure CSfast @10:58:31.94 CS.seek 7.500ml @10:58:38.48 CSV.dial :waste @10:58:39.74 CS.reconfigure CSconfig @10:58:39.77 CS.seek 0.000ml @10:58:59.58 Flushing lower manifold's waste1 port @10:58:59.67 CSV.dial :flush @10:59:00.10 CS.reconfigure CSfast @10:59:00.13 CS.seek 5.000ml @10:59:00.63 delay 30 seconds @10:59:12.32
delay 5 seconds @10:59:17.33 CSV.dial :air @10:59:18.01 CS.seek 7.000ml @10:59:23.39 CSV.dial CTV @10:59:23.41
CTV.dial CSV @10:59:24.73 CBV.dial :waste1 @10:59:25.47 CS.seek 0.000ml @10:59:30.64 ATV.dial :extSrc @10:59:31.27 delay 3 seconds @10:59:34.28 ATV.dial :ElutionWater @10:59:35.21 AS.seek 0.120ml @10:59:41.60 ATV.dial :air3 @10:59:42.09 AS.seek 0.130ml @10:59:42.75
Flushing lower manifold's waste2b port @10:59:42.85 CSV.dial :flush @10:59:43.65 CS.seek 5.000ml @10:59:44.38 delay 2 seconds @10:59:46.39 ATV.dial :bleach @10:59:46.77 AS.seek 0.230ml @10:59:55.85
delay 5 seconds @11:00:00.86 CSV.dial :air @11:00:01.40 CS.seek 7.000ml @11:00:06.85 CSV.dial CTV @11:00:06.87
CTV.dial CSV @11:00:07.32 CBV.dial :waste2b @11:00:07.41 delay 3 seconds @11:00:08.07
CS.seek 0.000ml @11:00:10.42 ATV.dial :air3 @11:00:10.86 delay 3 seconds @11:00:13.91 AS.seek 0.255ml @11:00:19.25 delay 3 seconds @11:00:22.26 ATV.dial :bleach @11:00:22.63 AS.seek 0.240ml @11:00:24.82
Flushing upper manifold @11:00:24.92 CSV.dial :flush @11:00:25.71 CS.seek 5.000ml @11:00:25.94 delay 3 seconds @11:00:28.95 ATV.dial :ASV @11:00:29.93 ASV.dial :SPEdelivery @11:00:30.25 AFV.select :carrierSolution @11:00:30.35 delay 2 seconds @11:00:32.36 AS.reconfigure ASconfig @11:00:32.39 AS.seek 0.500ml @11:00:37.95
delay 5 seconds @11:00:42.96 CSV.dial :air @11:00:43.51 CS.seek 7.001ml @11:00:47.83 delay 1 second @11:00:48.84 AFV.select :holdingCoil @11:00:48.92 CSV.dial CBV @11:00:48.93
CBV.dial CSV @11:00:49.06 AS.reconfigure ASslow1 @11:00:49.09 AS.seek 0.170ml @11:00:50.05
CTV.dial :air @11:00:51.33 CS.seek 0.000ml @11:01:07.91 delay 7 seconds @11:01:14.92 CS.seek 3.000ml @11:01:22.60 CSV.dial :waste @11:01:23.11 CS.seek 0.000ml @11:01:30.81 CSV.dial :flush @11:01:31.23 CS.seek 3.000ml @11:01:38.88 delay 5 seconds @11:01:43.89 CSV.dial :air @11:01:44.37 CS.seek 5.000ml @11:01:49.85 CSV.dial :air @11:01:49.87 CS.seek 0.000ml @11:01:56.68 SPE.hold 2:46:40,95.0C @11:01:56.70 delay 3:00 @11:02:02.00
delay 7 seconds @11:02:09.01 CS.seek 3.000ml @11:02:16.97 CSV.dial :waste @11:02:17.98 CS.seek 0.000ml @11:02:25.91 Disinfecting external Intake with mfbkill @11:02:26.00 CSV.dial CSR @11:02:27.71 CSR.select :mfbkill @11:02:27.81 CS.seek 8.000ml @11:02:47.56 delay 5 seconds @11:02:52.57 CSR.select :air @11:02:52.66 CS.seek 10.000ml @11:02:58.23 CSV.dial CBV @11:02:58.24
CBV.dial CSV @11:02:59.28 CTV.dial :intake @11:03:00.00 Intake.select :open @11:03:00.10 CS.seek 2.000ml @11:03:20.00 Intake.select :close @11:03:20.09 CTV.dial :air @11:03:20.87 CS.reconfigure CSconfig @11:03:20.90 CS.seek 0.000ml @11:03:26.78 Flushing lower manifold's waste1 port @11:03:26.79 CS Pulling 4ml of mfbkill @11:03:26.80 CSV.dial CSR @11:03:27.89 CSR.select :mfbkill @11:03:27.99 CS.seek 4.000ml @11:03:39.00 delay 5 seconds @11:03:44.01 CSR.select :air @11:03:44.10 CS.reconfigure CSfast @11:03:44.14 CS.seek 10.000ml @11:03:59.16 CSV.dial CTV @11:03:59.18
CTV.dial CSV @11:04:00.53 CBV.dial :waste1 @11:04:00.99 CS.seek 0.000ml @11:04:25.06 Flushing lower manifold's waste2b port @11:04:25.07 CS Pulling 4ml of mfbkill @11:04:25.08 CSV.dial CSR @11:04:25.59 CSR.select :mfbkill @11:04:25.69 CS.reconfigure CSconfig @11:04:25.73 CS.seek 4.000ml @11:04:36.84 delay 5 seconds @11:04:41.85 CSR.select :air @11:04:41.94 CS.reconfigure CSfast @11:04:41.98 CS.seek 10.000ml @11:04:56.71 AFV.select :carrierSolution @11:04:56.80 delay 2 seconds @11:04:57.43 CSV.dial CTV @11:04:57.46
CTV.dial CSV @11:04:57.99 CBV.dial :waste2b @11:04:58.76 CS.seek 0.000ml @11:04:59.42 AS.reconfigure ASconfig @11:04:59.46 AS.seek 1.000ml @11:05:22.99
Killing upper manifold @11:05:23.00 CS Pulling 4ml of mfbkill @11:05:23.01 CSV.dial CSR @11:05:23.92 CSR.select :mfbkill @11:05:24.02 CS.reconfigure CSconfig @11:05:24.06 CS.seek 4.000ml @11:05:34.93 delay 5 seconds @11:05:39.94 CSR.select :air @11:05:40.03 CS.reconfigure CSfast @11:05:40.07 CS.seek 10.000ml @11:05:48.10 delay 1 second @11:05:49.11 AFV.select :holdingCoil @11:05:49.20 delay 1 second @11:05:50.21 ASV.dial :wasteD @11:05:51.22 AS.seek 0.000ml @11:05:54.77 CSV.dial CBV @11:05:54.79
CBV.dial CSV @11:05:55.85 CTV.dial :air @11:05:57.14 CS.seek 0.000ml @11:06:20.96 delay 7 seconds @11:06:27.97 CS.seek 3.000ml @11:06:35.71 CSV.dial :waste @11:06:36.36 CS.seek 0.000ml @11:06:44.24 Flushing upper manifold @11:06:44.33 CSV.dial :flush @11:06:44.86 CS.seek 5.000ml @11:06:49.79 Priming Eluate @11:06:49.80 AFV.select :carrierSolution @11:06:49.89 delay 2 seconds @11:06:51.90 AS.seek 0.500ml @11:06:57.21
delay 5 seconds @11:07:02.22 CSV.dial :air @11:07:02.71 CS.seek 7.000ml @11:07:08.21 CSV.dial CBV @11:07:08.23
CBV.dial CSV @11:07:09.32 CTV.dial :air @11:07:09.34 CS.seek 0.000ml @11:07:21.38 delay 1 second @11:07:22.39 AFV.select :holdingCoil @11:07:22.48 ATV.dial ASV @11:07:22.49 ASV.dial :SPErecovery @11:07:23.41 Swish #1 of 2 @11:07:23.46 AS.reconfigure ASslow0 @11:07:23.50 AS.seek 0.600ml @11:07:26.05
delay 7 seconds @11:07:33.06 CS.seek 3.000ml @11:07:34.07 AS.seek 0.500ml @11:07:40.74
CSV.dial :waste @11:07:41.25 CS.seek 0.000ml @11:07:44.55 Swish #2 of 2 @11:07:44.61 AS.seek 0.600ml @11:07:49.04
CSV.dial :flush @11:07:49.70 CS.seek 3.000ml @11:07:55.12 AS.seek 0.500ml @11:07:57.52
delay 5 seconds @11:08:02.53 CSV.dial :air @11:08:03.10 CS.seek 5.000ml @11:08:05.60 delay 3 seconds @11:08:08.52
CSV.dial :air @11:08:08.54 CS.seek 0.000ml @11:08:08.66 AS.reconfigure ASslow1 @11:08:08.69 AS.seek 0.676ml @11:08:20.64
delay 7 seconds @11:08:27.65 CS.seek 3.000ml @11:08:35.36 CSV.dial :waste @11:08:36.35 CS.seek 0.000ml @11:08:43.95 Flushing lower manifold's waste1 port @11:08:44.04 CSV.dial :flush @11:08:44.48 CS.seek 5.000ml @11:08:44.86 delay 3 seconds @11:08:47.87 Eluate primed @11:08:47.88 ATV.dial :waste2 @11:08:48.42 AS.reconfigure ASnorm @11:08:48.45 AS.seek 0.000ml @11:08:56.70
delay 5 seconds @11:09:01.71 CSV.dial :air @11:09:02.58 CS.seek 7.000ml @11:09:07.98 CSV.dial CTV @11:09:08.00
CTV.dial CSV @11:09:09.37 CBV.dial :waste1 @11:09:09.82 CS.seek 0.000ml @11:09:23.28 SPE.coast @11:09:23.36 ASV.dial :SPErecovery @11:09:23.37 Setting up PCR for HF183em -- writing /var/log/esp/HF183em14mar0911h298ml.pcr @11:09:23.39 Building PCR Slug from ASV with MM2 mix, PP4 primer Priming with carrier @11:09:23.40 ATV.dial ARV @11:09:24.09 ARV.dial :carrier @11:09:24.38 AS.seek 0.500ml @11:09:26.48
Flushing lower manifold's waste2b port @11:09:26.57 CSV.dial :flush @11:09:27.96 CS.seek 5.000ml @11:09:40.11 delay 5 seconds @11:09:45.12 CSV.dial :air @11:09:45.67 CS.seek 7.000ml @11:09:50.31 AFV.select :carrierSolution @11:09:50.41 delay 2 seconds @11:09:51.02 CSV.dial CTV @11:09:51.04
CTV.dial CSV @11:09:51.48 CBV.dial :waste2b @11:09:52.23 CS.seek 0.000ml @11:09:52.42 AS.reconfigure ASconfig @11:09:52.46 AS.seek 1.000ml @11:10:08.73
Disinfecting external Intake with kill @11:10:08.82 CSV.dial CSR @11:10:09.25 CSR.select :kill @11:10:09.35 CS.seek 8.000ml @11:10:21.92 delay 1 second @11:10:22.93 AFV.select :holdingCoil @11:10:23.02 delay 1 second @11:10:24.03 ATV.dial :waste @11:10:25.29 AS.reconfigure ASnorm @11:10:25.32 AS.seek empty @11:10:28.23
delay 5 seconds @11:10:33.24 CSR.select :air @11:10:33.33 CS.seek 10.000ml @11:10:38.76 CSV.dial CBV @11:10:38.78
CBV.dial CSV @11:10:39.79 CTV.dial :intake @11:10:40.53 Intake.select :open @11:10:40.63 CS.seek 2.000ml @11:10:59.54 Intake.select :close @11:10:59.63 CTV.dial :air @11:11:00.35 CS.reconfigure CSconfig @11:11:00.38 CS.seek 0.000ml @11:11:06.35 CSV.dial CSR @11:11:07.40 CSR.select :flush @11:11:07.50 CS.seek 5.000ml @11:11:16.78 AFV.select :carrierSolution @11:11:16.89 delay 2 seconds @11:11:18.90 AS.reconfigure ASconfig @11:11:18.93 AS.seek 0.500ml @11:11:20.95
delay 5 seconds @11:11:25.96 CSR.select :air @11:11:26.05 CS.seek 10.000ml @11:11:39.48 CSV.dial CBV @11:11:40.53 CS.seek 0.000ml @11:11:48.45 delay 1 second @11:11:49.46 AFV.select :holdingCoil @11:11:49.55 Building Slug @11:11:49.56 ATV.dial :ARV @11:11:50.81 delay 2 seconds @11:11:52.82 ARV.dial :air1 @11:11:53.15 delay 2 seconds @11:11:55.21 AS.reconfigure ASslow2 @11:11:55.24 AS.seek 0.506ml @11:12:01.49 delay 2 seconds @11:12:03.50 ARV.dial :MM2 @11:12:04.71 delay 2 seconds @11:12:06.54
Garaging FlushPuck in Collection Clamp @11:12:06.60 Elbow.reconfigure TightElbow @11:12:06.63 Elbow.seek CC @11:12:06.73 Elbow.reconfigure LooseElbow @11:12:06.75 Adding MM2 @11:12:06.88 AS.seek 0.524ml @11:12:06.89
Forearm.seek CC @11:12:07.89 CC.seek sealed @11:12:08.79 Hand.grip @11:12:09.47 CC.seek open @11:12:12.78 Forearm.seek retract @11:12:13.72 Elbow.reconfigure TightElbow @11:12:13.75 Elbow.seek garage @11:12:16.54 Elbow.reconfigure LooseElbow @11:12:16.62 Forearm.reconfigure TightForearm @11:12:16.65 Forearm.seek garage @11:12:17.82 Hand.release @11:12:18.64 Forearm.reconfigure LooseForearm @11:12:18.67 Forearm.seek retract @11:12:25.45 delay 25 seconds @11:12:50.46 ARV.dial :PP4 @11:12:50.79 Adding PP4 @11:12:50.80 delay 2 seconds @11:12:52.86 AS.seek 0.530ml @11:12:59.21 delay 10 seconds @11:13:09.22 ARV.dial :air3 @11:13:09.52 adding ARV air @11:13:09.53 delay 2 seconds @11:13:11.59 AS.seek 0.536ml @11:13:18.10 delay 5 seconds @11:13:23.11 ARV.dial :carrier @11:13:24.13 delay 7 seconds @11:13:31.19 AS.seek 0.594ml @11:14:30.64 delay 5 seconds @11:14:35.65 ATV.dial :ASV @11:14:36.11 delay 2 seconds @11:14:38.17 AS.seek 0.600ml @11:14:45.08 delay 4 seconds @11:14:49.09 ATV.dial ARV @11:14:49.42 delay 2 seconds @11:14:51.43 ARV.dial :carrier @11:14:51.44 delay 2 seconds @11:14:53.49 AS.seek 0.650ml @11:15:44.61 delay 4 seconds @11:15:48.62 ATV.dial PCR @11:15:49.12 ASV.dial :waste1 @11:15:50.14 ARV.dial :air1 @11:15:50.48 Positioning Slug by 0.324ml @11:15:50.54 AS.seek 0.326ml @11:21:21.24 delay 5 seconds @11:21:26.25 ATV.dial ARV @11:21:26.84 ASV.dial :PCRlock @11:21:27.25 delay 5 seconds @11:21:32.26 Running HF183em ProfileEM60C2 thermocycling @11:21:32.27 PCR.program ProfileEM60C2 @11:21:32.38 ProfileEM60C2 should complete in < 2:11:38 @11:21:32.39 PCR.run 2:49:32.5 @13:18:47.08 PCR.off @13:18:47.60 PCR for HF183em completed -- closed /var/log/esp/HF183em14mar0911h298ml.pcr @13:18:47.65 Cleaning PCR @13:18:47.66 AFV.select :carrierSolution @13:18:47.75 delay 2 seconds @13:18:49.76 AS.reconfigure ASconfig @13:18:49.79 AS.seek 0.500ml @13:19:00.22 delay 1 second @13:19:01.23 AFV.select :holdingCoil @13:19:01.32 ASV.dial :waste1 @13:19:01.81 ATV.dial :air3 @13:19:02.75 AS.reconfigure ASslow1 @13:19:02.78 AS.seek 0.510ml @13:19:04.98 delay 2 seconds @13:19:06.99 ATV.dial :bleach @13:19:07.37 AS.seek 0.810ml @13:20:08.79 delay 3 seconds @13:20:11.80 ATV.dial :air3 @13:20:12.13 delay 3 seconds @13:20:15.19 AS.seek 0.835ml @13:20:20.53 delay 3 seconds @13:20:23.54 ATV.dial :bleach @13:20:23.93 AS.seek 0.820ml @13:20:27.23 delay 3 seconds @13:20:30.24 ARV.dial :waste @13:20:30.74 delay 3 seconds @13:20:34.35 ATV.dial :ARV @13:20:35.09 AS.reconfigure ASconfig @13:20:35.12 AS.seek 0.470ml @13:20:55.81 AFV.select :carrierSolution @13:20:55.90 delay 2 seconds @13:20:57.91 AS.seek 1.000ml @13:21:29.12 delay 1 second @13:21:30.13 AFV.select :holdingCoil @13:21:30.22 delay 1 second @13:21:31.23 AS.reconfigure ASfast @13:21:31.27 AS.seek 0.000ml @13:21:58.96 AFV.select :carrierSolution @13:21:59.06 delay 2 seconds @13:22:01.07 AS.reconfigure ASconfig @13:22:01.10 AS.seek 1.000ml @13:22:59.77 delay 1 second @13:23:00.78 AFV.select :holdingCoil @13:23:00.94 delay 1 second @13:23:01.95 AS.reconfigure ASfast @13:23:01.98 AS.seek 0.000ml @13:23:29.71 AFV.select :carrierSolution @13:23:29.81 delay 2 seconds @13:23:31.82 AS.reconfigure ASconfig @13:23:31.85 AS.seek 1.000ml @13:24:30.47 delay 1 second @13:24:31.48 AFV.select :holdingCoil @13:24:31.57 delay 1 second @13:24:32.58 AS.reconfigure ASfast @13:24:32.62 AS.seek 0.000ml @13:25:00.33 ARV.dial between carrier and air1 @13:25:00.73 ATV.dial :air3 @13:25:01.67 AS.reconfigure ASslow1 @13:25:01.70 AS.seek 0.010ml @13:25:04.11 delay 2 seconds @13:25:06.12 ATV.dial :bleach @13:25:06.52 AS.seek 0.810ml @13:27:49.92 delay 3 seconds @13:27:52.93 ATV.dial :air3 @13:27:53.28 delay 3 seconds @13:27:56.34 AS.seek 0.835ml @13:28:01.77 delay 3 seconds @13:28:04.78 ATV.dial :bleach @13:28:05.26 AS.seek 0.820ml @13:28:08.54 delay 3 seconds @13:28:11.55 ATV.dial PCR @13:28:11.89 AS.reconfigure ASconfig @13:28:11.92 AS.seek empty @13:29:00.05 PCR.program :bleachProfile @13:29:00.11 PCR program should complete in < 2:45 @13:29:00.12 PCR.run 10:00 @13:31:58.32 PCR.off @13:31:58.35 AFV.select :carrierSolution @13:31:58.44 delay 2 seconds @13:32:00.45 AS.seek 1.000ml @13:32:59.15 delay 1 second @13:33:00.16 AFV.select :holdingCoil @13:33:00.25 delay 1 second @13:33:01.26 AS.reconfigure ASfast @13:33:01.29 AS.seek 0.000ml @13:33:29.02 delay 3 seconds @13:33:32.03 AFV.select :carrierSolution @13:33:32.12 delay 2 seconds @13:33:34.13 AS.reconfigure ASconfig @13:33:34.16 AS.seek 1.000ml @13:34:32.85 delay 1 second @13:34:33.86 AFV.select :holdingCoil @13:34:33.95 delay 1 second @13:34:34.96 AS.reconfigure ASfast @13:34:34.99 AS.seek 0.000ml @13:35:02.77 delay 3 seconds @13:35:05.78 AFV.select :carrierSolution @13:35:05.87 delay 2 seconds @13:35:07.88 AS.reconfigure ASconfig @13:35:07.91 AS.seek 1.000ml @13:36:06.52 delay 1 second @13:36:07.53 AFV.select :holdingCoil @13:36:07.62 delay 1 second @13:36:08.63 AS.reconfigure ASfast @13:36:08.66 AS.seek 0.000ml @13:36:36.38 delay 3 seconds @13:36:39.39 AFV.select :carrierSolution @13:36:39.48 delay 2 seconds @13:36:41.49 AS.reconfigure ASconfig @13:36:41.52 AS.seek 1.000ml @13:37:40.19 delay 1 second @13:37:41.20 AFV.select :holdingCoil @13:37:41.29 delay 1 second @13:37:42.30 AS.reconfigure ASfast @13:37:42.33 AS.seek 0.000ml @13:38:10.04 delay 3 seconds @13:38:13.05 AFV.select :carrierSolution @13:38:13.14 delay 2 seconds @13:38:15.15 AS.reconfigure ASconfig @13:38:15.18 AS.seek 1.000ml @13:39:13.86 delay 1 second @13:39:14.87 AFV.select :holdingCoil @13:39:14.96 delay 1 second @13:39:15.97 AS.reconfigure ASnorm @13:39:16.00 AS.seek 0.000ml @13:40:07.45 Setting up PCR for ENTap -- writing /var/log/esp/ENTap14mar0913h298ml.pcr @13:40:07.46 Building PCR Slug from ASV with MM1 mix, PP1 primer @13:40:07.47 Priming with carrier @13:40:07.48 ATV.dial ARV @13:40:08.07 ARV.dial :carrier @13:40:08.36 AS.seek 0.500ml @13:40:34.31 AFV.select :carrierSolution @13:40:34.40 delay 2 seconds @13:40:36.41 AS.reconfigure ASconfig @13:40:36.44 AS.seek 1.000ml @13:41:05.84 delay 1 second @13:41:06.85 AFV.select :holdingCoil @13:41:06.94 delay 1 second @13:41:07.95 ATV.dial :waste @13:41:09.21 AS.reconfigure ASnorm @13:41:09.24 AS.seek empty @13:42:00.77 AFV.select :carrierSolution @13:42:00.87 delay 2 seconds @13:42:02.88 AS.reconfigure ASconfig @13:42:02.91 AS.seek 0.500ml @13:42:32.40 delay 1 second @13:42:33.41 AFV.select :holdingCoil @13:42:33.50 Building Slug @13:42:33.51 ATV.dial :ARV @13:42:34.75 delay 2 seconds @13:42:36.76 ARV.dial :air1 @13:42:37.08 delay 2 seconds @13:42:39.14 AS.reconfigure ASslow2 @13:42:39.17 AS.seek 0.506ml @13:42:45.47 delay 2 seconds @13:42:47.48 ARV.dial :MM1 @13:42:48.07 delay 2 seconds @13:42:50.64 Adding MM1 @13:42:50.70 AS.seek 0.524ml @13:43:09.25 delay 25 seconds @13:43:34.26 ARV.dial :PP1 @13:43:34.58 Adding PP1 @13:43:34.59 delay 2 seconds @13:43:36.64 AS.seek 0.530ml @13:43:43.14 delay 10 seconds @13:43:53.15 ARV.dial :air1 @13:43:53.47 adding ARV air @13:43:53.48 delay 2 seconds @13:43:55.53 AS.seek 0.536ml @13:44:01.77 delay 5 seconds @13:44:06.78 ARV.dial :carrier @13:44:07.10 delay 7 seconds @13:44:14.16 AS.seek 0.594ml @13:45:13.55 delay 5 seconds @13:45:18.56 ATV.dial :ASV @13:45:18.89 delay 2 seconds @13:45:20.95 AS.seek 0.600ml @13:45:27.37 delay 4 seconds @13:45:31.38 ATV.dial ARV @13:45:31.70 delay 2 seconds @13:45:33.71 ARV.dial :carrier @13:45:33.72 delay 2 seconds @13:45:35.77 AS.seek 0.650ml @13:46:26.97 delay 4 seconds @13:46:30.98 ATV.dial PCR @13:46:31.48 ASV.dial :waste1 @13:46:31.49 ARV.dial :air1 @13:46:31.83 Positioning Slug by 0.324ml @13:46:31.89 AS.seek 0.326ml @13:52:02.71 delay 5 seconds @13:52:07.72 ATV.dial ARV @13:52:08.23 ASV.dial :PCRlock @13:52:08.71 delay 5 seconds @13:52:13.72 Running ENTap Profile60C thermocycling @13:52:13.73 PCR.program Profile60C @13:52:13.83 Profile60C should complete in < 1:39:08 @13:52:13.84 PCR.run 2:08:55 @15:16:47.84 PCR.off @15:16:48.35 PCR for ENTap completed -- closed /var/log/esp/ENTap14mar0913h298ml.pcr @15:16:48.40 Cleaning PCR @15:16:48.41 AFV.select :carrierSolution @15:16:49.02 delay 2 seconds @15:16:51.03 AS.reconfigure ASconfig @15:16:51.07 AS.seek 0.500ml @15:17:01.48 delay 1 second @15:17:02.49 AFV.select :holdingCoil @15:17:02.58 ASV.dial :waste1 @15:17:03.07 ATV.dial :air3 @15:17:04.01 AS.reconfigure ASslow1 @15:17:04.04 AS.seek 0.510ml @15:17:06.35 delay 2 seconds @15:17:08.36 ATV.dial :bleach @15:17:08.73 AS.seek 0.810ml @15:18:10.15 delay 3 seconds @15:18:13.16 ATV.dial :air3 @15:18:13.59 delay 3 seconds @15:18:16.65 AS.seek 0.835ml @15:18:22.04 delay 3 seconds @15:18:25.05 ATV.dial :bleach @15:18:25.54 AS.seek 0.820ml @15:18:28.85 delay 3 seconds @15:18:31.86 ARV.dial :waste @15:18:32.37 delay 3 seconds @15:18:35.38 ATV.dial :ARV @15:18:36.13 AS.reconfigure ASconfig @15:18:36.16 AS.seek 0.470ml @15:18:56.85 AFV.select :carrierSolution @15:18:56.96 delay 2 seconds @15:18:58.97 AS.seek 1.000ml @15:19:30.16 delay 1 second @15:19:31.17 AFV.select :holdingCoil @15:19:31.26 delay 1 second @15:19:32.27 AS.reconfigure ASfast @15:19:32.30 AS.seek 0.000ml @15:19:59.99 AFV.select :carrierSolution @15:20:00.09 delay 2 seconds @15:20:02.10 AS.reconfigure ASconfig @15:20:02.13 AS.seek 1.000ml @15:21:00.78 delay 1 second @15:21:01.79 AFV.select :holdingCoil @15:21:01.88 delay 1 second @15:21:02.89 AS.reconfigure ASfast @15:21:02.92 AS.seek 0.000ml @15:21:30.64 AFV.select :carrierSolution @15:21:30.74 delay 2 seconds @15:21:32.75 AS.reconfigure ASconfig @15:21:32.78 AS.seek 1.000ml @15:22:31.42 delay 1 second @15:22:32.43 AFV.select :holdingCoil @15:22:32.52 delay 1 second @15:22:33.53 AS.reconfigure ASfast @15:22:33.56 AS.seek 0.000ml @15:23:01.29 ARV.dial between carrier and air1 @15:23:01.70 ATV.dial :air3 @15:23:02.63 AS.reconfigure ASslow1 @15:23:02.66 AS.seek 0.010ml @15:23:05.09 delay 2 seconds @15:23:07.10 ATV.dial :bleach @15:23:07.47 AS.seek 0.810ml @15:25:50.85 delay 3 seconds @15:25:53.86 ATV.dial :air3 @15:25:54.27 delay 3 seconds @15:25:57.33 AS.seek 0.835ml @15:26:02.68 delay 3 seconds @15:26:05.69 ATV.dial :bleach @15:26:06.18 AS.seek 0.820ml @15:26:09.48 delay 3 seconds @15:26:12.49 ATV.dial PCR @15:26:12.83 AS.reconfigure ASconfig @15:26:12.86 AS.seek empty @15:27:01.08 PCR.program :bleachProfile @15:27:01.13 PCR program should complete in < 2:45 @15:27:01.14 PCR.run 10:00 @15:29:59.33 PCR.off @15:29:59.36 AFV.select :carrierSolution @15:29:59.45 delay 2 seconds @15:30:01.46 AS.seek 1.000ml @15:31:00.13 delay 1 second @15:31:01.14 AFV.select :holdingCoil @15:31:01.23 delay 1 second @15:31:02.24 AS.reconfigure ASfast @15:31:02.27 AS.seek 0.000ml @15:31:30.02 delay 3 seconds @15:31:33.03 AFV.select :carrierSolution @15:31:33.12 delay 2 seconds @15:31:35.13 AS.reconfigure ASconfig @15:31:35.16 AS.seek 1.000ml @15:32:33.78 delay 1 second @15:32:34.79 AFV.select :holdingCoil @15:32:34.88 delay 1 second @15:32:35.89 AS.reconfigure ASfast @15:32:35.92 AS.seek 0.000ml @15:33:03.63 delay 3 seconds @15:33:06.64 AFV.select :carrierSolution @15:33:06.73 delay 2 seconds @15:33:08.74 AS.reconfigure ASconfig @15:33:08.77 AS.seek 1.000ml @15:34:07.42 delay 1 second @15:34:08.43 AFV.select :holdingCoil @15:34:08.52 delay 1 second @15:34:09.53 AS.reconfigure ASfast @15:34:09.56 AS.seek 0.000ml @15:34:37.28 delay 3 seconds @15:34:40.29 AFV.select :carrierSolution @15:34:40.38 delay 2 seconds @15:34:42.39 AS.reconfigure ASconfig @15:34:42.42 AS.seek 1.000ml @15:35:41.04 delay 1 second @15:35:42.05 AFV.select :holdingCoil @15:35:42.14 delay 1 second @15:35:43.15 AS.reconfigure ASfast @15:35:43.18 AS.seek 0.000ml @15:36:10.87 delay 3 seconds @15:36:13.88 AFV.select :carrierSolution @15:36:13.97 delay 2 seconds @15:36:15.98 AS.reconfigure ASconfig @15:36:16.01 AS.seek 1.000ml @15:37:14.60 delay 1 second @15:37:15.61 AFV.select :holdingCoil @15:37:15.70 delay 1 second @15:37:16.71 AS.reconfigure ASnorm @15:37:16.74 AS.seek 0.000ml @15:38:08.15 Setting up PCR for ENTem -- writing /var/log/esp/ENTem14mar0915h298ml.pcr @15:38:08.16 Building PCR Slug from ASV with MM2 mix, PP3 primer @15:38:08.17 Priming with carrier @15:38:08.18 ATV.dial ARV @15:38:08.68 ARV.dial :carrier @15:38:08.96 AS.seek 0.500ml @15:38:34.88 AFV.select :carrierSolution @15:38:34.97 delay 2 seconds @15:38:37.54 AS.reconfigure ASconfig @15:38:37.58 AS.seek 1.000ml @15:39:07.04 delay 1 second @15:39:08.05 AFV.select :holdingCoil @15:39:08.15 delay 1 second @15:39:09.16 ATV.dial :waste @15:39:10.41 AS.reconfigure ASnorm @15:39:10.44 AS.seek empty @15:40:01.86 AFV.select :carrierSolution @15:40:01.96 delay 2 seconds @15:40:03.97 AS.reconfigure ASconfig @15:40:04.01 AS.seek 0.500ml @15:40:33.55 delay 1 second @15:40:34.56 AFV.select :holdingCoil @15:40:34.66 Building Slug @15:40:34.67 ATV.dial :ARV @15:40:35.91 delay 2 seconds @15:40:37.92 ARV.dial :air1 @15:40:38.25 delay 2 seconds @15:40:40.31 AS.reconfigure ASslow2 @15:40:40.34 AS.seek 0.506ml @15:40:46.70 delay 2 seconds @15:40:48.71 ARV.dial :MM2 @15:40:50.00 delay 2 seconds @15:40:52.01 Adding MM2 @15:40:52.07 AS.seek 0.524ml @15:41:10.52 delay 25 seconds @15:41:35.53 ARV.dial :PP3 @15:41:35.96 Adding PP3 @15:41:35.97 delay 2 seconds @15:41:38.02 AS.seek 0.530ml @15:41:44.48 delay 10 seconds @15:41:54.49 ARV.dial :air2 @15:41:54.82 adding ARV air @15:41:54.83 delay 2 seconds @15:41:56.88 AS.seek 0.536ml @15:42:03.33 delay 5 seconds @15:42:08.34 ARV.dial :carrier @15:42:09.37 delay 7 seconds @15:42:16.43 AS.seek 0.594ml @15:43:15.79 delay 5 seconds @15:43:20.80 ATV.dial :ASV @15:43:21.12 delay 2 seconds @15:43:23.18 AS.seek 0.600ml @15:43:29.81 delay 4 seconds @15:43:33.82 ATV.dial ARV @15:43:34.15 delay 2 seconds @15:43:36.16 ARV.dial :carrier @15:43:36.17 delay 2 seconds @15:43:38.22 AS.seek 0.650ml @15:44:29.51 delay 4 seconds @15:44:33.52 ATV.dial PCR @15:44:34.03 ASV.dial :waste1 @15:44:34.04 ARV.dial :air1 @15:44:34.38 Positioning Slug by 0.324ml @15:44:34.44 AS.seek 0.326ml @15:50:04.96 delay 5 seconds @15:50:09.97 ATV.dial ARV @15:50:10.48 ASV.dial :PCRlock @15:50:11.11 delay 5 seconds @15:50:16.12 Running ENTem ProfileEM60C thermocycling @15:50:16.14 PCR.program ProfileEM60C @15:50:16.24 ProfileEM60C should complete in < 2:11:38 @15:50:16.25 PCR.run 2:49:32.5 @17:48:04.11 PCR.off @17:48:04.65 PCR for ENTem completed -- closed /var/log/esp/ENTem14mar0915h298ml.pcr @17:48:04.70 Cleaning PCR @17:48:04.71 AFV.select :carrierSolution @17:48:04.80 delay 2 seconds @17:48:06.81 AS.reconfigure ASconfig @17:48:06.84 AS.seek 0.500ml @17:48:17.27 delay 1 second @17:48:18.28 AFV.select :holdingCoil @17:48:18.37 ASV.dial :waste1 @17:48:18.85 ATV.dial :air3 @17:48:19.79 AS.reconfigure ASslow1 @17:48:19.82 AS.seek 0.510ml @17:48:22.10 delay 2 seconds @17:48:24.11 ATV.dial :bleach @17:48:24.49 AS.seek 0.810ml @17:49:25.86 delay 3 seconds @17:49:28.87 ATV.dial :air3 @17:49:29.19 delay 3 seconds @17:49:32.25 AS.seek 0.835ml @17:49:37.61 delay 3 seconds @17:49:40.62 ATV.dial :bleach @17:49:40.99 AS.seek 0.820ml @17:49:44.28 delay 3 seconds @17:49:47.29 ARV.dial :waste @17:49:47.78 delay 3 seconds @17:49:50.79 ATV.dial :ARV @17:49:51.52 AS.reconfigure ASconfig @17:49:51.55 AS.seek 0.470ml @17:50:12.24 AFV.select :carrierSolution @17:50:12.33 delay 2 seconds @17:50:14.34 AS.seek 1.000ml @17:50:45.55 delay 1 second @17:50:46.56 AFV.select :holdingCoil @17:50:46.65 delay 1 second @17:50:47.66 AS.reconfigure ASfast @17:50:47.69 AS.seek 0.000ml @17:51:15.37 AFV.select :carrierSolution @17:51:15.46 delay 2 seconds @17:51:17.47 AS.reconfigure ASconfig @17:51:17.50 AS.seek 1.000ml @17:52:16.14 delay 1 second @17:52:17.15 AFV.select :holdingCoil @17:52:17.24 delay 1 second @17:52:18.25 AS.reconfigure ASfast @17:52:18.28 AS.seek 0.000ml @17:52:46.00 AFV.select :carrierSolution @17:52:46.10 delay 2 seconds @17:52:48.11 AS.reconfigure ASconfig @17:52:48.14 AS.seek 1.000ml @17:53:46.74 delay 1 second @17:53:47.75 AFV.select :holdingCoil @17:53:47.84 delay 1 second @17:53:48.85 AS.reconfigure ASfast @17:53:48.88 AS.seek 0.000ml @17:54:16.73 ARV.dial between carrier and air1 @17:54:17.13 ATV.dial :air3 @17:54:18.06 AS.reconfigure ASslow1 @17:54:18.09 AS.seek 0.010ml @17:54:20.40 delay 2 seconds @17:54:22.41 ATV.dial :bleach @17:54:22.79 AS.seek 0.810ml @17:57:06.24 delay 3 seconds @17:57:09.25 ATV.dial :air3 @17:57:09.69 delay 3 seconds @17:57:12.75 AS.seek 0.835ml @17:57:18.08 delay 3 seconds @17:57:21.09 ATV.dial :bleach @17:57:21.47 AS.seek 0.820ml @17:57:24.77 delay 3 seconds @17:57:27.78 ATV.dial PCR @17:57:28.11 AS.reconfigure ASconfig @17:57:28.14 AS.seek empty @17:58:16.27 PCR.program :bleachProfile @17:58:16.32 PCR program should complete in < 2:45 @17:58:16.33 PCR.run 10:00 @18:01:14.52 PCR.off @18:01:14.55 AFV.select :carrierSolution @18:01:14.64 delay 2 seconds @18:01:16.65 AS.seek 1.000ml @18:02:15.37 delay 1 second @18:02:16.38 AFV.select :holdingCoil @18:02:16.47 delay 1 second @18:02:17.48 AS.reconfigure ASfast @18:02:17.51 AS.seek 0.000ml @18:02:45.23 delay 3 seconds @18:02:48.24 AFV.select :carrierSolution @18:02:48.33 delay 2 seconds @18:02:50.34 AS.reconfigure ASconfig @18:02:50.37 AS.seek 1.000ml @18:03:49.03 delay 1 second @18:03:50.04 AFV.select :holdingCoil @18:03:50.13 delay 1 second @18:03:51.14 AS.reconfigure ASfast @18:03:51.17 AS.seek 0.000ml @18:04:18.89 delay 3 seconds @18:04:21.90 AFV.select :carrierSolution @18:04:21.99 delay 2 seconds @18:04:24.00 AS.reconfigure ASconfig @18:04:24.03 AS.seek 1.000ml @18:05:22.65 delay 1 second @18:05:23.66 AFV.select :holdingCoil @18:05:23.75 delay 1 second @18:05:25.35 AS.reconfigure ASfast @18:05:25.38 AS.seek 0.000ml @18:05:53.21 delay 3 seconds @18:05:56.22 AFV.select :carrierSolution @18:05:56.31 delay 2 seconds @18:05:58.32 AS.reconfigure ASconfig @18:05:58.35 AS.seek 1.000ml @18:06:56.96 delay 1 second @18:06:57.97 AFV.select :holdingCoil @18:06:58.06 delay 1 second @18:06:59.07 AS.reconfigure ASfast @18:06:59.10 AS.seek 0.000ml @18:07:26.79 delay 3 seconds @18:07:29.80 AFV.select :carrierSolution @18:07:29.89 delay 2 seconds @18:07:31.90 AS.reconfigure ASconfig @18:07:31.93 AS.seek 1.000ml @18:08:30.60 delay 1 second @18:08:31.61 AFV.select :holdingCoil @18:08:31.70 delay 1 second @18:08:32.71 AS.reconfigure ASnorm @18:08:32.74 AS.seek 0.000ml @18:09:24.19 Setting up PCR for IPCap -- writing /var/log/esp/IPCap14mar0918h298ml.pcr @18:09:24.20 Building PCR Slug from ASV with MM1 mix, PP2 primer @18:09:24.21 Priming with carrier @18:09:24.22 ATV.dial ARV @18:09:24.74 ARV.dial :carrier @18:09:25.02 AS.seek 0.500ml @18:09:50.97 AFV.select :carrierSolution @18:09:51.08 delay 2 seconds @18:09:53.09 AS.reconfigure ASconfig @18:09:53.13 AS.seek 1.000ml @18:10:22.56 delay 1 second @18:10:23.57 AFV.select :holdingCoil @18:10:23.66 delay 1 second @18:10:24.67 ATV.dial :waste @18:10:25.93 AS.reconfigure ASnorm @18:10:25.96 AS.seek empty @18:11:17.39 AFV.select :carrierSolution @18:11:17.49 delay 2 seconds @18:11:19.50 AS.reconfigure ASconfig @18:11:19.53 AS.seek 0.500ml @18:11:49.06 delay 1 second @18:11:50.07 AFV.select :holdingCoil @18:11:50.16 Building Slug @18:11:50.17 ATV.dial :ARV @18:11:51.40 delay 2 seconds @18:11:53.41 ARV.dial :air1 @18:11:53.73 delay 2 seconds @18:11:55.79 AS.reconfigure ASslow2 @18:11:55.82 AS.seek 0.506ml @18:12:02.15 delay 2 seconds @18:12:04.16 ARV.dial :MM1 @18:12:04.75 delay 2 seconds @18:12:06.76 Adding MM1 @18:12:06.82 AS.seek 0.524ml @18:12:25.36 delay 25 seconds @18:12:50.37 ARV.dial :PP2 @18:12:50.79 Adding PP2 @18:12:50.81 delay 2 seconds @18:12:52.86 AS.seek 0.530ml @18:12:59.18 delay 10 seconds @18:13:09.19 ARV.dial :air2 @18:13:09.60 adding ARV air @18:13:09.61 delay 2 seconds @18:13:11.67 AS.seek 0.536ml @18:13:18.26 delay 5 seconds @18:13:23.27 ARV.dial :carrier @18:13:24.29 delay 7 seconds @18:13:31.35 AS.seek 0.594ml @18:14:30.66 delay 5 seconds @18:14:35.67 ATV.dial :ASV @18:14:35.99 delay 2 seconds @18:14:38.05 AS.seek 0.600ml @18:14:44.38 delay 4 seconds @18:14:48.39 ATV.dial ARV @18:14:48.71 delay 2 seconds @18:14:50.72 ARV.dial :carrier @18:14:50.73 delay 2 seconds @18:14:52.78 AS.seek 0.650ml @18:15:44.02 delay 4 seconds @18:15:48.03 ATV.dial PCR @18:15:48.52 ASV.dial :waste1 @18:15:48.53 ARV.dial :air1 @18:15:48.88 Positioning Slug by 0.324ml @18:15:48.93 AS.seek 0.326ml @18:21:19.65 delay 5 seconds @18:21:24.66 ATV.dial ARV @18:21:25.18 ASV.dial :PCRlock @18:21:25.68 delay 5 seconds @18:21:30.69 Running IPCap Profile59C thermocycling @18:21:30.70 PCR.program Profile59C @18:21:30.80 Profile59C should complete in < 1:39:08 @18:21:30.81 PCR.run 2:08:55 @19:46:38.45 PCR.off @19:46:38.99 PCR for IPCap completed -- closed /var/log/esp/IPCap14mar0918h298ml.pcr @19:46:39.04 Cleaning PCR AFV.select :carrierSolution @19:46:39.13 delay 2 seconds @19:46:41.14 AS.reconfigure ASconfig @19:46:41.17 AS.seek 0.500ml @19:46:51.57 delay 1 second @19:46:52.58 AFV.select :holdingCoil @19:46:52.67 ASV.dial :waste1 @19:46:53.43 ATV.dial :air3 @19:46:54.37 AS.reconfigure ASslow1 @19:46:54.40 AS.seek 0.510ml @19:46:56.69 delay 2 seconds @19:46:58.70 ATV.dial :bleach @19:46:59.18 AS.seek 0.810ml @19:48:00.61 delay 3 seconds @19:48:03.62 ATV.dial :air3 @19:48:03.94 delay 3 seconds @19:48:07.00 AS.seek 0.835ml @19:48:12.36 delay 3 seconds @19:48:15.37 ATV.dial :bleach @19:48:15.85 AS.seek 0.820ml @19:48:19.14 delay 3 seconds @19:48:22.15 ARV.dial :waste @19:48:22.74 delay 3 seconds @19:48:25.75 ATV.dial :ARV @19:48:26.50 AS.reconfigure ASconfig @19:48:26.54 AS.seek 0.470ml @19:48:47.24 AFV.select :carrierSolution @19:48:47.33 delay 2 seconds @19:48:49.34 AS.seek 1.000ml @19:49:20.48 delay 1 second @19:49:21.49 AFV.select :holdingCoil @19:49:21.58 delay 1 second @19:49:22.59 AS.reconfigure ASfast @19:49:22.62 AS.seek 0.000ml @19:49:50.44 AFV.select :carrierSolution @19:49:50.54 delay 2 seconds @19:49:52.55 AS.reconfigure ASconfig @19:49:52.59 AS.seek 1.000ml @19:50:51.20 delay 1 second @19:50:52.21 AFV.select :holdingCoil @19:50:52.30 delay 1 second @19:50:53.31 AS.reconfigure ASfast @19:50:53.34 AS.seek 0.000ml @19:51:21.06 AFV.select :carrierSolution @19:51:21.16 delay 2 seconds @19:51:23.17 AS.reconfigure ASconfig @19:51:23.20 AS.seek 1.000ml @19:52:21.88 delay 1 second @19:52:22.89 AFV.select :holdingCoil @19:52:22.98 delay 1 second @19:52:23.99 AS.reconfigure ASfast @19:52:24.02 AS.seek 0.000ml @19:52:51.71 ARV.dial between carrier and air1 @19:52:52.12 ATV.dial :air3 @19:52:53.14 AS.reconfigure ASslow1 @19:52:53.17 AS.seek 0.010ml @19:52:55.59 delay 2 seconds @19:52:57.60 ATV.dial :bleach @19:52:58.09 AS.seek 0.810ml @19:55:41.55 delay 3 seconds @19:55:44.56 ATV.dial :air3 @19:55:44.89 delay 3 seconds @19:55:47.95 AS.seek 0.835ml @19:55:53.36 delay 3 seconds @19:55:56.37 ATV.dial :bleach @19:55:56.76 AS.seek 0.820ml @19:56:00.05 delay 3 seconds @19:56:03.06 ATV.dial PCR @19:56:03.39 AS.reconfigure ASconfig @19:56:03.42 AS.seek empty @19:56:51.61 PCR.program :bleachProfile @19:56:51.66 PCR program should complete in < 2:45 @19:56:51.67 PCR.run 10:00 @19:59:50.47 PCR.off @19:59:50.50 AFV.select :carrierSolution @19:59:50.59 delay 2 seconds @19:59:52.60 AS.seek 1.000ml @20:00:51.27 delay 1 second @20:00:52.28 AFV.select :holdingCoil @20:00:52.37 delay 1 second @20:00:53.38 AS.reconfigure ASfast @20:00:53.42 AS.seek 0.000ml @20:01:21.16 delay 3 seconds @20:01:24.17 AFV.select :carrierSolution @20:01:24.26 delay 2 seconds @20:01:26.27 AS.reconfigure ASconfig @20:01:26.30 AS.seek 1.000ml @20:02:24.93 delay 1 second @20:02:25.94 AFV.select :holdingCoil @20:02:26.03 delay 1 second @20:02:27.04 AS.reconfigure ASfast @20:02:27.08 AS.seek 0.000ml @20:02:54.80 delay 3 seconds @20:02:57.81 AFV.select :carrierSolution @20:02:57.90 delay 2 seconds @20:02:59.91 AS.reconfigure ASconfig @20:02:59.94 AS.seek 1.000ml @20:03:58.59 delay 1 second @20:03:59.60 AFV.select :holdingCoil @20:03:59.69 delay 1 second @20:04:00.70 AS.reconfigure ASfast @20:04:00.73 AS.seek 0.000ml @20:04:28.46 delay 3 seconds @20:04:31.47 AFV.select :carrierSolution @20:04:31.56 delay 2 seconds @20:04:33.57 AS.reconfigure ASconfig @20:04:33.60 AS.seek 1.000ml @20:05:32.26 delay 1 second @20:05:33.27 AFV.select :holdingCoil @20:05:33.36 delay 1 second @20:05:34.37 AS.reconfigure ASfast @20:05:34.40 AS.seek 0.000ml @20:06:02.09 delay 3 seconds @20:06:05.10 AFV.select :carrierSolution @20:06:05.19 delay 2 seconds @20:06:07.20 AS.reconfigure ASconfig @20:06:07.23 AS.seek 1.000ml @20:07:05.85 delay 1 second @20:07:06.86 AFV.select :holdingCoil @20:07:06.95 delay 1 second @20:07:07.96 AS.reconfigure ASnorm @20:07:07.99 AS.seek 0.000ml @20:07:59.39 Setting up PCR for IPCem -- writing /var/log/esp/IPCem14mar0920h298ml.pcr @20:07:59.40 Building PCR Slug from ASV with MM3 mix, PP5 primer @20:07:59.41 Priming with carrier @20:07:59.42 ATV.dial ARV @20:08:00.00 ARV.dial :carrier @20:08:00.29 AS.seek 0.500ml @20:08:26.21 AFV.select :carrierSolution @20:08:26.32 delay 2 seconds @20:08:28.33 AS.reconfigure ASconfig @20:08:28.36 AS.seek 1.000ml @20:08:57.78 delay 1 second @20:08:58.79 AFV.select :holdingCoil @20:08:58.88 delay 1 second @20:08:59.89 ATV.dial :waste @20:09:01.14 AS.reconfigure ASnorm @20:09:01.17 AS.seek empty @20:09:52.62 AFV.select :carrierSolution @20:09:52.72 delay 2 seconds @20:09:54.73 AS.reconfigure ASconfig @20:09:54.76 AS.seek 0.500ml @20:10:24.31 delay 1 second @20:10:25.32 AFV.select :holdingCoil @20:10:25.41 Building Slug @20:10:25.42 ATV.dial :ARV @20:10:26.66 delay 2 seconds @20:10:28.67 ARV.dial :air1 @20:10:29.00 delay 2 seconds @20:10:31.06 AS.reconfigure ASslow2 @20:10:31.09 AS.seek 0.506ml @20:10:37.37 delay 2 seconds @20:10:39.38 ARV.dial :MM3 @20:10:40.23 delay 2 seconds @20:10:42.24 Adding MM3 @20:10:42.30 AS.seek 0.524ml @20:11:00.94 delay 25 seconds @20:11:25.95 ARV.dial :PP5 @20:11:26.26 Adding PP5 @20:11:26.28 delay 2 seconds @20:11:28.33 AS.seek 0.530ml @20:11:34.62 delay 10 seconds @20:11:44.63 ARV.dial :air3 @20:11:45.04 adding ARV air @20:11:45.05 delay 2 seconds @20:11:47.11 AS.seek 0.536ml @20:11:53.54 delay 5 seconds @20:11:58.55 ARV.dial :carrier @20:11:59.56 delay 7 seconds @20:12:06.62 AS.seek 0.594ml @20:13:05.87 delay 5 seconds @20:13:10.88 ATV.dial :ASV @20:13:11.29 delay 2 seconds @20:13:13.35 AS.seek 0.600ml @20:13:19.73 delay 4 seconds @20:13:23.74 ATV.dial ARV @20:13:24.07 delay 2 seconds @20:13:26.08 ARV.dial :carrier @20:13:26.09 delay 2 seconds @20:13:28.15 AS.seek 0.650ml @20:14:19.40 delay 4 seconds @20:14:23.41 ATV.dial PCR @20:14:23.91 ASV.dial :waste1 @20:14:23.92 ARV.dial :air1 @20:14:24.25 Positioning Slug by 0.324ml @20:14:24.30 AS.seek 0.326ml @20:19:54.98 delay 5 seconds @20:20:00.00 ATV.dial ARV @20:20:00.52 ASV.dial :PCRlock @20:20:00.97 delay 5 seconds @20:20:05.98 Running IPCem ProfileEM59C thermocycling @20:20:05.99 PCR.program ProfileEM59C @20:20:06.10 ProfileEM59C should complete in < 2:11:38 @20:20:06.11 PCR.run 2:49:32.5 @22:18:19.12 PCR.off @22:18:19.65 PCR for IPCem completed -- closed /var/log/esp/IPCem14mar0920h298ml.pcr @22:18:19.67 Full MFB Decontamination Decon extraction column and decon AIV @22:18:19.69 SPE.hold 2:46:40,55.0C @22:18:19.70 ASV.dial :SPEdelivery @22:18:20.36 delay 20 seconds @22:18:40.37 AFV.select :carrierSolution @22:18:40.46 delay 2 seconds @22:18:42.47 AS.reconfigure ASconfig @22:18:42.50 AS.seek 0.500ml @22:18:52.90 delay 1 second @22:18:53.91 AFV.select :holdingCoil @22:18:54.00 ATV.dial :air1 @22:18:55.50 AS.reconfigure ASslow1 @22:18:55.53 AS.seek 0.520ml @22:18:59.86 ATV.dial :ASV @22:19:01.12 ASV.dial :SPErecovery @22:19:01.67 AS.seek 0.620ml @22:19:22.33 ATV.dial :waste @22:19:23.87 AS.reconfigure ASnorm @22:19:23.90 AS.seek empty @22:19:55.91 AFV.select :holdingCoil @22:19:55.92 ATV.dial :waste @22:19:55.93 AS.reconfigure ASconfig @22:19:55.96 AS.seek 0.000ml @22:19:56.27 AFV.select :carrierSolution @22:19:56.36 delay 2 seconds @22:19:58.37 AS.seek 0.500ml @22:20:27.78 delay 1 second @22:20:28.79 AFV.select :holdingCoil @22:20:28.88 ATV.dial :air3 @22:20:29.43 AS.reconfigure ASslow1 @22:20:29.46 AS.seek 0.520ml @22:20:33.85 ATV.dial :air3 @22:20:33.91 AS.seek 0.530ml @22:20:36.21 delay 2 seconds @22:20:38.22 ATV.dial :bleach @22:20:38.60 AS.seek 0.710ml @22:21:15.58 delay 3 seconds @22:21:18.59 ATV.dial :air3 @22:21:19.02 delay 3 seconds @22:21:22.08 AS.seek 0.735ml @22:21:27.42 delay 3 seconds @22:21:30.43 ATV.dial :bleach @22:21:30.91 AS.seek 0.720ml @22:21:34.22 delay 3 seconds @22:21:37.23 ATV.dial ASV @22:21:38.22 ASV.dial :SPErecovery @22:21:38.28 AS.seek 0.365ml @22:22:50.92 ATV.dial :waste @22:22:53.09 AS.reconfigure ASnorm @22:22:53.12 AS.seek empty @22:23:12.14 ATV.dial :air3 @22:23:12.70 AS.reconfigure ASslow1 @22:23:12.73 AS.seek 0.009ml @22:23:15.14 delay 2 seconds @22:23:17.15 ATV.dial :bleach @22:23:17.52 AS.seek 0.579ml @22:25:14.01 delay 3 seconds @22:25:17.02 ATV.dial :air3 @22:25:17.35 delay 3 seconds @22:25:20.41 AS.seek 0.604ml @22:25:25.77 delay 3 seconds @22:25:28.78 ATV.dial :bleach @22:25:29.15 AS.seek 0.589ml @22:25:32.46 delay 3 seconds @22:25:35.47 ATV.dial :ASV @22:25:36.34 ASV.dial :wasteR @22:25:36.85 AS.seek 0.000ml @22:27:37.29 delay 1:00 @22:28:37.30 ATV.dial ASV @22:28:37.31 AFV.select :carrierSolution @22:28:37.40 delay 2 seconds @22:28:39.41 AS.reconfigure ASconfig @22:28:39.44 AS.seek 1.000ml @22:29:38.06 delay 1 second @22:29:39.07 AFV.select :holdingCoil @22:29:39.16 delay 1 second @22:29:40.17 ASV.dial :wasteR @22:29:40.18 AS.reconfigure ASslow1 @22:29:40.21 AS.seek 0.000ml @22:33:04.70 delay 10 seconds @22:33:14.71 ATV.dial :air3 @22:33:15.82 AS.seek 0.010ml @22:33:18.13 delay 2 seconds @22:33:20.14 ATV.dial :bleach @22:33:20.62 AS.seek 0.580ml @22:35:17.10 delay 3 seconds @22:35:20.11 ATV.dial :air3 @22:35:20.44 delay 3 seconds @22:35:23.50 AS.seek 0.605ml @22:35:28.77 delay 3 seconds @22:35:31.78 ATV.dial :bleach @22:35:32.18 AS.seek 0.590ml @22:35:35.49 delay 3 seconds @22:35:38.50 ATV.dial ASV @22:35:39.36 ASV.dial :SPEdelivery @22:35:39.69 AS.seek 0.000ml @22:37:40.53 delay 1:00 @22:38:40.54 AFV.select :carrierSolution @22:38:40.63 delay 2 seconds @22:38:42.64 AS.reconfigure ASconfig @22:38:42.67 AS.seek 1.000ml @22:39:41.29 delay 1 second @22:39:42.30 AFV.select :holdingCoil @22:39:42.39 delay 1 second @22:39:43.40 ATV.dial ASV @22:39:43.41 ASV.dial :SPEdelivery @22:39:43.42 AS.reconfigure ASslow1 @22:39:43.45 AS.seek 0.000ml @22:43:07.73 delay 10 seconds @22:43:17.74 ATV.dial :air2 @22:43:18.45 AS.reconfigure ASslow0 @22:43:18.48 AS.seek 0.800ml @22:44:40.50 delay 3 seconds @22:44:43.51 ATV.dial :ASV @22:44:44.21 ASV.dial :SPEdelivery @22:44:44.23 AS.reconfigure ASslow1 @22:44:44.26 AS.seek 0.030ml @22:47:21.63 delay 20 seconds @22:47:41.64 ATV.dial :waste,via => air3 @22:47:43.08 AS.reconfigure ASconfig @22:47:43.11 AS.seek empty @22:47:45.32 SPE.coast @22:47:45.40 Removing Bleach from Mixing Coil @22:47:45.41 AFV.select :carrierSolution @22:47:45.50 delay 2 seconds @22:47:47.51 AS.seek 0.500ml @22:48:17.00 delay 1 second @22:48:18.01 AFV.select :holdingCoil @22:48:18.10 ATV.dial :ASV @22:48:19.63 ASV.dial :SPErecovery @22:48:20.31 AS.reconfigure ASslow1 @22:48:20.35 AS.seek 0.730ml @22:49:07.55 ASV.dial :wasteD @22:49:08.06 AS.reconfigure ASnorm @22:49:08.09 AS.seek 0.000ml @22:49:45.69 AFV.select :carrierSolution @22:49:45.79 delay 2 seconds @22:49:47.80 AS.reconfigure ASconfig @22:49:47.83 AS.seek 0.500ml @22:50:17.31 delay 1 second @22:50:18.32 AFV.select :holdingCoil @22:50:18.41 ATV.dial ASV @22:50:18.42 ASV.dial :SPErecovery @22:50:19.13 AS.reconfigure ASslow0 @22:50:19.16 AS.seek 0.300ml @22:50:39.86 ATV.dial :air2 @22:50:40.59 AS.reconfigure ASslow1 @22:50:40.63 AS.seek 0.320ml @22:50:45.00 ATV.dial ASV @22:50:45.75 AS.reconfigure ASslow0 @22:50:45.78 AS.seek 0.570ml @22:51:11.50 ASV.dial :wasteD @22:51:12.43 AS.reconfigure ASnorm @22:51:12.46 AS.seek 0.000ml @22:51:41.87 AFV.select :carrierSolution @22:51:41.97 delay 2 seconds @22:51:43.98 AS.reconfigure ASconfig @22:51:44.01 AS.seek 0.500ml @22:52:13.50 delay 1 second @22:52:14.51 AFV.select :holdingCoil @22:52:14.60 ATV.dial ASV @22:52:14.61 ASV.dial :SPErecovery @22:52:15.14 AS.reconfigure ASslow0 @22:52:15.17 AS.seek 0.300ml @22:52:35.85 ATV.dial :air2 @22:52:36.61 AS.reconfigure ASslow1 @22:52:36.64 AS.seek 0.320ml @22:52:40.99 ATV.dial ASV @22:52:41.83 AS.reconfigure ASslow0 @22:52:41.87 AS.seek 0.570ml @22:53:08.20 ASV.dial :wasteD @22:53:08.85 AS.reconfigure ASnorm @22:53:08.88 AS.seek 0.000ml @22:53:38.38 ATV.dial :air2 @22:53:39.08 AS.reconfigure ASslow0 @22:53:39.11 AS.seek 0.300ml @22:54:10.14 ATV.dial :ASV @22:54:10.85 ASV.dial :wasteD @22:54:10.87 AS.reconfigure ASconfig @22:54:10.90 AS.seek 0.050ml @22:54:25.78 ATV.dial :waste @22:54:27.20 AS.seek 0.000ml @22:54:30.45 ASV.dial :SPEdelivery @22:54:31.45 deconLysateINT @22:54:31.46 ATV.dial :air3 @22:54:32.02 AS.reconfigure ASslow1 @22:54:32.05 AS.seek 0.010ml @22:54:34.41 delay 2 seconds @22:54:36.42 ATV.dial :bleach @22:54:36.80 AS.seek 0.960ml @22:57:50.78 delay 3 seconds @22:57:53.79 ATV.dial :air3 @22:57:54.21 delay 3 seconds @22:57:57.27 AS.seek 0.985ml @22:58:02.60 delay 3 seconds @22:58:05.61 ATV.dial :bleach @22:58:05.98 AS.seek 0.970ml @22:58:09.31 delay 3 seconds @22:58:12.32 ATV.dial :AIV @22:58:13.38 AIV.dial :coilToMFB @22:58:13.39 AWV.select :waste @22:58:13.40 AFV.select :carrierSolution @22:58:13.49 delay 2 seconds @22:58:15.50 AS.reconfigure ASconfig @22:58:15.53 AS.seek 1.000ml @22:58:17.59 delay 1 second @22:58:18.60 AFV.select :holdingCoil @22:58:18.69 delay 1 second @22:58:19.70 AS.reconfigure ASslow0 @22:58:19.73 AS.seek empty @23:00:02.30 AFV.select :carrierSolution @23:00:02.40 delay 2 seconds @23:00:04.41 AS.reconfigure ASconfig @23:00:04.44 AS.seek 1.000ml @23:01:03.08 delay 1 second @23:01:04.09 AFV.select :holdingCoil @23:01:04.18 delay 1 second @23:01:05.19 AS.reconfigure ASslow0 @23:01:05.22 AS.seek empty @23:02:47.83 AFV.select :carrierSolution @23:02:47.93 delay 2 seconds @23:02:49.94 AS.reconfigure ASconfig @23:02:49.98 AS.seek 1.000ml @23:03:48.58 delay 1 second @23:03:49.59 AFV.select :holdingCoil @23:03:49.68 delay 1 second @23:03:50.69 AS.reconfigure ASslow0 @23:03:50.72 AS.seek empty @23:05:33.06 ATV.dial :air2 @23:05:33.62 AS.reconfigure ASslow1 @23:05:33.65 AS.seek 0.009ml @23:05:36.07 AS.reconfigure ASslow0 @23:05:36.11 AS.seek 1.000ml @23:07:17.43 ATV.dial AIV @23:07:17.97 AS.seek 0.020ml @23:08:58.22 ATV.dial :waste @23:08:59.58 AS.seek 0.000ml @23:09:01.88 AWV.select :air @23:09:02.03 Cleaning PCR @23:09:02.04 AFV.select :carrierSolution @23:09:02.13 delay 2 seconds @23:09:04.14 AS.reconfigure ASconfig @23:09:04.17 AS.seek 0.500ml @23:09:33.66 delay 1 second @23:09:34.67 AFV.select :holdingCoil @23:09:34.76 ASV.dial :waste1 @23:09:35.10 ATV.dial :air3 @23:09:35.75 AS.reconfigure ASslow1 @23:09:35.78 AS.seek 0.510ml @23:09:38.11 delay 2 seconds @23:09:40.12 ATV.dial :bleach @23:09:40.49 AS.seek 0.810ml @23:10:42.01 delay 3 seconds @23:10:45.02 ATV.dial :air3 @23:10:45.35 delay 3 seconds @23:10:48.41 AS.seek 0.835ml @23:10:53.82 delay 3 seconds @23:10:56.83 ATV.dial :bleach @23:10:57.20 AS.seek 0.820ml @23:11:00.48 delay 3 seconds @23:11:03.49 ARV.dial :waste @23:11:03.99 delay 3 seconds @23:11:07.00 ATV.dial :ARV @23:11:07.83 AS.reconfigure ASconfig @23:11:07.87 AS.seek 0.470ml @23:11:28.54 AFV.select :carrierSolution @23:11:28.64 delay 2 seconds @23:11:30.65 AS.seek 1.000ml @23:12:01.88 delay 1 second @23:12:02.89 AFV.select :holdingCoil @23:12:02.98 delay 1 second @23:12:03.99 AS.reconfigure ASfast @23:12:04.02 AS.seek 0.000ml @23:12:31.77 AFV.select :carrierSolution @23:12:31.87 delay 2 seconds @23:12:33.88 AS.reconfigure ASconfig @23:12:33.91 AS.seek 1.000ml @23:13:32.58 delay 1 second @23:13:33.59 AFV.select :holdingCoil @23:13:33.68 delay 1 second @23:13:34.69 AS.reconfigure ASfast @23:13:34.72 AS.seek 0.000ml @23:14:02.47 AFV.select :carrierSolution @23:14:02.57 delay 2 seconds @23:14:04.58 AS.reconfigure ASconfig @23:14:04.61 AS.seek 1.000ml @23:15:03.22 delay 1 second @23:15:04.23 AFV.select :holdingCoil @23:15:04.32 delay 1 second @23:15:05.33 AS.reconfigure ASfast @23:15:05.36 AS.seek 0.000ml @23:15:33.20 ARV.dial between carrier and air1 @23:15:33.61 ATV.dial :air3 @23:15:34.60 AS.reconfigure ASslow1 @23:15:34.64 AS.seek 0.010ml @23:15:36.97 delay 2 seconds @23:15:38.98 ATV.dial :bleach @23:15:39.91 AS.seek 0.810ml @23:18:23.34 delay 3 seconds @23:18:26.35 ATV.dial :air3 @23:18:26.68 delay 3 seconds @23:18:29.74 AS.seek 0.835ml @23:18:35.09 delay 3 seconds @23:18:38.10 ATV.dial :bleach @23:18:38.48 AS.seek 0.820ml @23:18:41.77 delay 3 seconds @23:18:44.78 ATV.dial PCR @23:18:45.11 AS.reconfigure ASconfig @23:18:45.14 AS.seek empty @23:19:33.29 PCR.program :bleachProfile @23:19:33.35 PCR program should complete in < 2:45 @23:19:33.36 PCR.run 10:00 @23:22:37.59 PCR.off @23:22:37.62 AFV.select :carrierSolution @23:22:37.71 delay 2 seconds @23:22:39.72 AS.seek 1.000ml @23:23:38.44 delay 1 second @23:23:39.45 AFV.select :holdingCoil @23:23:39.54 delay 1 second @23:23:40.55 AS.reconfigure ASfast @23:23:40.58 AS.seek 0.000ml @23:24:08.33 delay 3 seconds @23:24:11.34 AFV.select :carrierSolution @23:24:11.43 delay 2 seconds @23:24:13.44 AS.reconfigure ASconfig @23:24:13.47 AS.seek 1.000ml @23:25:12.14 delay 1 second @23:25:13.15 AFV.select :holdingCoil @23:25:13.24 delay 1 second @23:25:14.25 AS.reconfigure ASfast @23:25:14.28 AS.seek 0.000ml @23:25:41.99 delay 3 seconds @23:25:45.00 AFV.select :carrierSolution @23:25:45.09 delay 2 seconds @23:25:47.10 AS.reconfigure ASconfig @23:25:47.13 AS.seek 1.000ml @23:26:45.81 delay 1 second @23:26:46.82 AFV.select :holdingCoil @23:26:46.91 delay 1 second @23:26:47.92 AS.reconfigure ASfast @23:26:47.95 AS.seek 0.000ml @23:27:15.69 delay 3 seconds @23:27:18.70 AFV.select :carrierSolution @23:27:18.79 delay 2 seconds @23:27:20.80 AS.reconfigure ASconfig @23:27:20.83 AS.seek 1.000ml @23:28:19.47 delay 1 second @23:28:20.48 AFV.select :holdingCoil @23:28:20.57 delay 1 second @23:28:21.58 AS.reconfigure ASfast @23:28:21.61 AS.seek 0.000ml @23:28:49.34 delay 3 seconds @23:28:52.35 AFV.select :carrierSolution @23:28:52.44 delay 2 seconds @23:28:54.45 AS.reconfigure ASconfig @23:28:54.48 AS.seek 1.000ml @23:29:53.14 delay 1 second @23:29:54.15 AFV.select :holdingCoil @23:29:54.24 delay 1 second @23:29:55.25 AS.reconfigure ASnorm @23:29:55.28 AS.seek 0.000ml @23:30:46.67 AFV.select :carrierSolution @23:30:46.76 delay 2 seconds @23:30:48.77 AS.reconfigure ASconfig @23:30:48.80 AS.seek 1.000ml @23:31:47.45 delay 1 second @23:31:48.46 AFV.select :holdingCoil @23:31:48.55 delay 1 second @23:31:49.56 ATV.dial :waste @23:31:50.46 AS.reconfigure ASnorm @23:31:50.49 AS.seek 0.500ml @23:32:16.38 ATV.dial :waste2 @23:32:17.45 AS.seek 0.000ml @23:32:43.35
Cleaning PCR AFV.select :carrierSolution @23:32:43.44 delay 2 seconds @23:32:45.45 AS.reconfigure ASconfig @23:32:45.48 AS.seek 0.500ml @23:33:14.96 delay 1 second @23:33:15.97 AFV.select :holdingCoil @23:33:16.06 ASV.dial :waste1 @23:33:16.07 ATV.dial :air3 @23:33:17.65 AS.reconfigure ASslow1 @23:33:17.68 AS.seek 0.510ml @23:33:19.88 delay 2 seconds @23:33:21.89 ATV.dial :bleach @23:33:22.27 AS.seek 0.810ml @23:34:23.71 delay 3 seconds @23:34:26.73 ATV.dial :air3 @23:34:27.13 delay 3 seconds @23:34:30.19 AS.seek 0.835ml @23:34:35.52 delay 3 seconds @23:34:38.54 ATV.dial :bleach @23:34:38.91 AS.seek 0.820ml @23:34:42.22 delay 3 seconds @23:34:45.23 ARV.dial :waste @23:34:45.63 delay 3 seconds @23:34:48.64 ATV.dial :ARV @23:34:49.37 AS.reconfigure ASconfig @23:34:49.41 AS.seek 0.470ml @23:35:10.08 AFV.select :carrierSolution @23:35:10.17 delay 2 seconds @23:35:12.18 AS.seek 1.000ml @23:35:43.38 delay 1 second @23:35:44.40 AFV.select :holdingCoil @23:35:44.49 delay 1 second @23:35:45.50 AS.reconfigure ASfast @23:35:45.53 AS.seek 0.000ml @23:36:13.25 AFV.select :carrierSolution @23:36:13.34 delay 2 seconds @23:36:15.35 AS.reconfigure ASconfig @23:36:15.38 AS.seek 1.000ml @23:37:14.04 delay 1 second @23:37:15.06 AFV.select :holdingCoil @23:37:15.15 delay 1 second @23:37:16.16 AS.reconfigure ASfast @23:37:16.19 AS.seek 0.000ml @23:37:43.90 AFV.select :carrierSolution @23:37:43.99 delay 2 seconds @23:37:46.00 AS.reconfigure ASconfig @23:37:46.03 AS.seek 1.000ml @23:38:44.68 delay 1 second @23:38:45.70 AFV.select :holdingCoil @23:38:45.79 delay 1 second @23:38:46.80 AS.reconfigure ASfast @23:38:46.85 AS.seek 0.000ml @23:39:14.56 ARV.dial between carrier and air1 @23:39:15.50 ATV.dial :air3 @23:39:16.43 AS.reconfigure ASslow1 @23:39:16.46 AS.seek 0.010ml @23:39:18.85 delay 2 seconds @23:39:20.86 ATV.dial :bleach @23:39:21.34 AS.seek 0.810ml @23:42:04.77 delay 3 seconds @23:42:07.78 ATV.dial :air3 @23:42:08.11 delay 3 seconds @23:42:11.17 AS.seek 0.835ml @23:42:16.52 delay 3 seconds @23:42:19.53 ATV.dial :bleach @23:42:19.93 AS.seek 0.820ml @23:42:23.24 delay 3 seconds @23:42:26.25 ATV.dial PCR @23:42:26.58 AS.reconfigure ASconfig @23:42:26.61 AS.seek empty @23:43:14.74 PCR.program :bleachProfile @23:43:14.80 PCR program should complete in < 2:45 @23:43:14.81 PCR.run 10:00 @23:46:16.03 PCR.off @23:46:16.06 AFV.select :carrierSolution @23:46:16.15 delay 2 seconds @23:46:18.16 AS.seek 1.000ml @23:47:16.88 delay 1 second @23:47:17.89 AFV.select :holdingCoil @23:47:17.98 delay 1 second @23:47:18.99 AS.reconfigure ASfast @23:47:19.02 AS.seek 0.000ml @23:47:46.85 delay 3 seconds @23:47:49.86 AFV.select :carrierSolution @23:47:49.95 delay 2 seconds @23:47:51.96 AS.reconfigure ASconfig @23:47:51.99 AS.seek 1.000ml @23:48:50.56 delay 1 second @23:48:51.58 AFV.select :holdingCoil @23:48:51.67 delay 1 second @23:48:52.68 AS.reconfigure ASfast @23:48:52.71 AS.seek 0.000ml @23:49:20.45 delay 3 seconds @23:49:23.46 AFV.select :carrierSolution @23:49:23.55 delay 2 seconds @23:49:25.56 AS.reconfigure ASconfig @23:49:25.59 AS.seek 1.000ml @23:50:24.25 delay 1 second @23:50:25.27 AFV.select :holdingCoil @23:50:25.36 delay 1 second @23:50:26.37 AS.reconfigure ASfast @23:50:26.41 AS.seek 0.000ml @23:50:54.09 delay 3 seconds @23:50:57.10 AFV.select :carrierSolution @23:50:57.19 delay 2 seconds @23:50:59.20 AS.reconfigure ASconfig @23:50:59.23 AS.seek 1.000ml @23:51:57.85 delay 1 second @23:51:58.87 AFV.select :holdingCoil @23:51:58.96 delay 1 second @23:51:59.97 AS.reconfigure ASfast @23:52:00.00 AS.seek 0.000ml @23:52:27.70 delay 3 seconds @23:52:30.71 AFV.select :carrierSolution @23:52:30.80 delay 2 seconds @23:52:32.81 AS.reconfigure ASconfig @23:52:32.84 AS.seek 1.000ml @23:53:31.49 delay 1 second @23:53:32.50 AFV.select :holdingCoil @23:53:32.59 delay 1 second @23:53:33.60 AS.reconfigure ASnorm @23:53:33.63 AS.seek 0.000ml @23:54:25.04 Completed {wqPCR} begun 09:02:05 @23:54:25.07 Asleep til 09:02PDT10-Mar-14 @23:54:25.08 AS.stop @23:54:25.09
Sleeping until 09:02PDT10-Mar-14 @23:54:25.12 GoodNews.email "Then, in tube 2: wqPCR\n/home/esp/esp2local/mission/14mar05sccwrpDeploy.rb:64",Subject => "Asleep until 09:02PDT10-Mar-14" @23:54:26.78 CC.stop @23:54:26.84 CS.stop @23:54:26.87 PC.stop @23:54:26.90 PS.stop @23:54:26.93 Forearm.stop @23:54:26.96 Elbow.stop @23:54:26.99 SS.stop @23:54:27.06 Sleepy.power :raw,:OFF @23:54:27.07 Sleepy.power :dwarf,:OFF Shutdown @09:02:05.79PDT10-Mar-14
Powering up Dwarves @09:02:10.58 Sleepy.power :raw,:ON @09:02:11.10 SPE.configure SPEconfig @09:02:11.16 AS.configure ASconfig @09:02:11.19 CC.configure CCconfig @09:02:11.22 CS.configure CSconfig @09:02:11.25 PC.configure PCconfig @09:02:11.28 PS.configure PSconfig @09:02:11.31 Forearm.configure LooseForearm @09:02:11.34 Elbow.configure LooseElbow @09:02:11.37 SC.configure SCloose @09:02:11.40 SE.configure SEconfig @09:02:11.43 SS.configure SSconfig @09:02:11.46 CH.configure CHconfig @09:02:11.48 PH.configure PHconfig @09:02:11.51 Valve.reset @09:02:12.09 PCR.off @09:02:12.12 PCR.pidtemp= 50,0.1,0,300,25,25 @09:02:12.15 PCR.caltemp= 6.6661,0.0344,0.0 @09:02:12.25 Intake.select :close @09:02:12.28 Exhaust.select :close @09:02:12.29 CTV.dial :air @09:02:12.33 PSV.dial :air @09:02:12.36
PC.reconfigure PCconfig @09:02:12.41 PC.home.seek open @09:02:12.46 CSV.dial :waste1 @09:02:12.87 SSV.dial :spit,avoiding => exhaust @09:02:12.96 PS.reconfigure PSconfig @09:02:12.99 PS.home.seek 0.000ml @09:02:13.68 CS.reconfigure CSfast @09:02:13.71 SS.reconfigure SSconfig @09:02:13.81 CS.home.seek 0.000ml @09:02:13.84 SS.home.seek 0.00ml @09:02:13.90 CC.reconfigure CCconfig @09:02:13.93 CC.home.seek open @09:02:23.21 PS.coast @09:02:23.29
CC.seek open @09:02:23.31 PC.seek open @09:02:23.47
Forearm.reconfigure LooseForearm @09:02:23.50 Forearm.home.seek retract @09:02:24.23 Hand.grip @09:02:24.25
Elbow.jog -1500 @09:02:24.78 Elbow.reconfigure LooseElbow @09:02:24.81 Elbow.home.seek clear @09:02:29.55 SE.reconfigure SEconfig @09:02:29.58 SE.home.seek down @09:02:30.12 SC.reconfigure SCsloppy @09:02:30.16 SC.home.seek initial @09:02:32.35 Forearm.seek retract @09:02:32.41 Elbow.seek hammer @09:02:33.60 Forearm.reconfigure TightForearm @09:02:33.65 Forearm.seek 2 @09:02:34.81 Forearm.seek retract @09:02:35.80 Forearm.reconfigure LooseForearm @09:02:36.31PDT10-Mar-14 ===> STARTED wqPCR (phase #7) <=== @09:02:36.32 Starting initialPurge @09:02:36.33 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @09:02:36.38 Hand.release @09:02:37.26 Elbow.reconfigure TightElbow @09:02:37.29 Elbow.seek FlushPuck::Garage @09:02:39.03 Elbow.reconfigure LooseElbow @09:02:39.11 Forearm.seek garage @09:02:40.09 Hand.grip @09:02:40.91 Forearm.seek retract @09:02:41.94 Elbow.reconfigure TightElbow @09:02:41.97 Elbow.seek CC @09:02:44.77 Elbow.reconfigure LooseElbow @09:02:44.86 Forearm.reconfigure TightForearm @09:02:44.89 Forearm.seek CC @09:02:46.01 CC.seek closed @09:02:49.76 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.95 @09:02:49.83 Hand.release @09:02:50.59 Forearm.reconfigure LooseForearm @09:02:50.62 Forearm.seek retract @09:02:51.55 Flushing the Collection Valve::Manifold @09:02:51.64 CS Pulling 5ml of flush @09:02:51.65 CSV.dial CSR @09:02:52.92 CSR.select :flush @09:02:53.02 CS.reconfigure CSconfig @09:02:53.06 CS.seek 5.000ml @09:03:06.59 delay 5 seconds @09:03:11.60 CSR.select :air @09:03:11.74 CS.reconfigure CSfast @09:03:11.77 CS.seek 7.500ml @09:03:18.34 CSV.dial :waste @09:03:19.59 CS.reconfigure CSconfig @09:03:19.63 CS.seek 0.000ml @09:03:39.49 Purging external Intake @09:03:39.58 CSV.dial :flush @09:03:40.02 CS.reconfigure CSfast @09:03:40.05 CS.seek 10.000ml @09:04:03.74 delay 5 seconds @09:04:08.76 CSV.dial CBV @09:04:08.78
CBV.dial CSV @09:04:09.57 CTV.dial :intake @09:04:10.32 Intake.select :open @09:04:10.41 CS.seek 0.000ml @09:04:34.71 Intake.select :close @09:04:34.80 CTV.dial :air @09:04:35.54 Flushing upper manifold @09:04:35.63 CSV.dial :flush @09:04:36.42 CS.seek 5.000ml @09:04:48.71 delay 5 seconds @09:04:53.72 CSV.dial :air @09:04:54.20 CS.seek 7.000ml @09:04:59.65 CSV.dial CBV @09:04:59.67
CBV.dial CSV @09:05:00.74 CTV.dial :air @09:05:00.76 CS.seek 0.000ml @09:05:17.58 delay 7 seconds @09:05:24.59 CS.seek 3.000ml @09:05:32.33 CSV.dial :waste @09:05:32.84 CS.seek 0.000ml @09:05:40.60 CSV.dial :flush @09:05:41.03 CS.seek 3.000ml @09:05:48.74 delay 5 seconds @09:05:53.75 CSV.dial :air @09:05:54.23 CS.seek 5.000ml @09:05:59.61 CSV.dial :air @09:05:59.62 CS.seek 0.000ml @09:06:11.86 delay 7 seconds @09:06:18.87 CS.seek 3.000ml @09:06:26.77 CSV.dial :waste @09:06:27.56 CS.seek 0.000ml @09:06:35.24 Garaging FlushPuck in Collection Clamp @09:06:35.30 Elbow.reconfigure TightElbow @09:06:35.33 Elbow.seek CC @09:06:35.55 Elbow.reconfigure LooseElbow @09:06:35.63 Forearm.seek CC @09:06:36.57 CC.seek sealed @09:06:37.49 Hand.grip @09:06:38.29 CC.seek open @09:06:41.58 Forearm.seek retract @09:06:42.53 Elbow.reconfigure TightElbow @09:06:42.57 Elbow.seek garage @09:06:45.96 Elbow.reconfigure LooseElbow @09:06:46.05 Forearm.reconfigure TightForearm @09:06:46.08 Forearm.seek garage @09:06:47.22 Hand.release @09:06:47.95 Forearm.reconfigure LooseForearm @09:06:47.98 Forearm.seek retract @09:06:49.09 Starting mfb @09:06:49.10 pcrBAC sampling at most 1000ml, wcr at most 1000ml @09:06:49.12 Loading sh1 puck from tube 2 into Collection Clamp @09:06:49.15 BACfans.email "Can@09:02:36, 15.9C, 65% humidity, 14.2psia, 13.719V, 0.646A, 0.640A avg, 8.87W\nCTD data unavailable\nISUS data unavailable",Subject => "pcrBAC sampling at most 1000ml, wcr at most 1000ml" @09:06:50.79 Readying the MFB @09:06:50.80 Homing MFB Syringe AFV.select :holdingCoil @09:06:51.07 ATV.dial :air2 @09:06:51.09
SC.reconfigure SCloose @09:06:51.30 Elbow.reconfigure TightElbow @09:06:51.33 Elbow.seek 2 @09:06:52.40 AS.reconfigure ASconfig @09:06:52.43 AS.home.seek home @09:06:53.16
Forearm.reconfigure TightForearm @09:06:53.19 Forearm.seek 2 + 800 counts @09:06:54.41 SE.reconfigure SEpick @09:06:54.46 SE.seek beamBreak @09:06:57.10 SE.configure SEconfig @09:06:57.23 Elbow.seek 2 @09:06:57.38 Forearm.seek 2 + 300 counts @09:06:57.65 Forearm.reconfigure LooseForearm @09:06:57.69 Hand.grip @09:06:58.43 SE.seek down @09:06:58.56
Forearm.seek retract @09:06:59.57 Elbow.seek CC @09:07:00.33 AFV.select :carrierSolution @09:07:00.43 delay 2 seconds @09:07:00.85 SE.coast @09:07:01.12
Elbow.reconfigure LooseElbow @09:07:01.21 Forearm.reconfigure TightForearm @09:07:01.24 Forearm.seek CC @09:07:02.38 CC.seek closed @09:07:02.44 AS.seek 1.000ml @09:07:06.16
Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.95 @09:07:06.17 Hand.release @09:07:06.95 Forearm.reconfigure LooseForearm @09:07:06.98 Forearm.seek retract @09:07:07.90 SC.coast @09:07:07.93 collectSample 1000ml w/bubble point of 55psi Priming 7ml @09:07:07.94 Sleepy.power :sampler,:ON @09:07:07.95 Intake.select :close Exhaust.select :close @09:07:07.96 SS.hold @09:07:08.12 Calibrating pressure and temperature sensors @09:07:08.14 CTV.dial :air @09:07:08.15 SSV.dial :spit,avoiding => exhaust @09:07:08.16 SS.reconfigure SSfast @09:07:08.19 SS.seek 5.00ml @09:07:11.04 SSV.dial CBV @09:07:11.06
CBV.dial SSV @09:07:12.44 delay 3.8 seconds @09:07:16.32 SSV.dial :spit @09:07:17.67 SS.seek 0.00ml @09:07:20.30 SSV.dial CBV @09:07:21.65 CTV.dial :intake @09:07:22.42 Waiting for external sampler to finish priming... @09:07:22.43 delayUntil "09:17:07.94" @09:07:54.20 delay 1 second @09:07:55.21 AFV.select :holdingCoil @09:07:55.30 delay 1 second @09:07:56.31 ATV.dial :waste @09:07:57.77 AS.reconfigure ASnorm @09:07:57.80 AS.home.seek empty @09:08:51.07 ATV.dial :waste @09:08:51.08 ARV.dial between carrier and air1 @09:08:51.15 ASV.dial :waste1 @09:17:07.95 SSV.dial CBV @09:17:07.97
CBV.dial SSV @09:17:07.98 Allowing 55s for 7.0ml of remaining 7.0ml Intake.select :open @09:17:10.11 Intake @51.7psia, vacuum from 20.0 to 24.0psi @09:17:10.12 SS.reconfigure forSampling @09:17:10.16 SS.seek 7.00ml @09:17:15.88 Stroke completed -- waiting for pressure to equalize ... @09:17:16.52 Intake.select :close @09:17:16.67 SSV.dial :exhaust @09:17:17.44 Exhaust.select :open @09:17:17.54 SS.reconfigure SSexhale @09:17:17.58 SS.seek 0.00ml @09:17:21.49 Exhaust.select :close @09:17:21.59 Sampling 1000.0ml @09:17:21.60 Intake.select :close @09:17:21.61 Exhaust.select :close @09:17:21.62 SS.reconfigure SSconfig @09:17:21.65 SS.hold @09:17:21.81 Calibrating pressure sensors @09:17:21.82 CTV.dial :air @09:17:22.59 SSV.dial :spit,avoiding => exhaust @09:17:23.36 SS.reconfigure SSfast @09:17:23.39 SS.seek 5.00ml @09:17:25.91 SSV.dial CBV @09:17:25.93
CBV.dial SSV @09:17:27.43 delay 3.8 seconds @09:17:31.31 SSV.dial :spit @09:17:32.67 SS.seek 0.00ml @09:17:35.26 SSV.dial CBV @09:17:36.58 CTV.dial :intake @09:17:37.34 CBV.dial SSV @09:17:37.35 SSV.dial CBV @09:17:37.36
Allowing 185s for 25.0ml of remaining 1000.0ml @09:17:37.37 Intake.select :open @09:17:39.50 Intake @51.5psia, vacuum from 20.0 to 24.0psi @09:17:39.51 SS.reconfigure forSampling @09:17:39.55 SS.seek 25.00ml @09:17:44.94 Vacuum too high -- waiting up to 40s @09:17:45.21 Intake @44.4psia, vacuum from 20.0 to 24.0psi @09:17:45.22 SS.reconfigure forSampling @09:17:45.25 SS.seek 25.00ml @09:17:54.94 Stroke completed -- waiting for pressure to equalize ... @09:17:55.58 Intake.select :close @09:17:55.71 SSV.dial :exhaust @09:17:56.49 Exhaust.select :open @09:17:56.59 SS.reconfigure SSexhale @09:17:56.63 SS.seek 0.00ml @09:18:05.85 Exhaust.select :close @09:18:05.94 SSV.dial CBV @09:18:05.96
CBV.dial SSV @09:18:06.72 Allowing 185s for 25.0ml of remaining 975.0ml @09:18:06.73 Intake.select :open @09:18:08.86 Intake @49.6psia, vacuum from 20.0 to 24.0psi @09:18:08.87 SS.reconfigure forSampling @09:18:08.91 SS.seek 25.00ml @09:18:15.00 Vacuum too high -- waiting up to 40s @09:18:15.27 Intake @44.5psia, vacuum from 20.0 to 24.0psi @09:18:15.28 SS.reconfigure forSampling @09:18:15.31 SS.seek 25.00ml @09:18:26.77 Stroke completed -- waiting for pressure to equalize ... @09:18:27.41 Intake.select :close @09:18:27.54 SSV.dial :exhaust @09:18:28.32 Exhaust.select :open @09:18:28.41 SS.reconfigure SSexhale @09:18:28.45 SS.seek 0.00ml @09:18:37.46 Exhaust.select :close @09:18:37.55 SSV.dial CBV @09:18:37.57
CBV.dial SSV @09:18:38.35 Allowing 185s for 25.0ml of remaining 950.0ml @09:18:38.36 Intake.select :open @09:18:40.49 Intake @48.3psia, vacuum from 20.0 to 24.0psi @09:18:40.50 SS.reconfigure forSampling @09:18:40.54 SS.seek 25.00ml @09:18:44.22 Vacuum too high -- waiting up to 40s @09:18:44.48 Intake @45.4psia, vacuum from 20.0 to 24.0psi @09:18:44.49 SS.reconfigure forSampling @09:18:44.53 SS.seek 25.00ml @09:18:53.03 Vacuum too high -- waiting up to 40s @09:18:53.30 Intake @45.1psia, vacuum from 20.0 to 24.0psi @09:18:53.31 SS.reconfigure forSampling @09:18:53.34 SS.seek 25.00ml @09:19:01.95 Stroke completed -- waiting for pressure to equalize ... @09:19:02.59 Intake.select :close @09:19:02.72 SSV.dial :exhaust @09:19:03.49 Exhaust.select :open @09:19:03.58 SS.reconfigure SSexhale @09:19:03.62 SS.seek 0.00ml @09:19:12.77 Exhaust.select :close @09:19:12.86 SSV.dial CBV @09:19:12.88
CBV.dial SSV @09:19:13.66 Allowing 185s for 25.0ml of remaining 925.0ml @09:19:13.67 Intake.select :open @09:19:15.80 Intake @47.0psia, vacuum from 20.0 to 24.0psi @09:19:15.81 SS.reconfigure forSampling @09:19:15.85 SS.seek 25.00ml @09:19:18.79 Vacuum too high -- waiting up to 40s @09:19:19.04 Intake @44.3psia, vacuum from 20.0 to 24.0psi @09:19:19.05 SS.reconfigure forSampling @09:19:19.09 SS.seek 25.00ml @09:19:28.65 Vacuum too high -- waiting up to 40s @09:19:28.92 Intake @45.3psia, vacuum from 20.0 to 24.0psi @09:19:28.93 SS.reconfigure forSampling @09:19:28.96 SS.seek 25.00ml @09:19:38.03 Vacuum too high -- waiting up to 40s @09:19:38.30 Intake @44.4psia, vacuum from 20.0 to 24.0psi @09:19:38.31 SS.reconfigure forSampling @09:19:38.34 SS.seek 25.00ml @09:19:50.28 Stroke completed -- waiting for pressure to equalize ... @09:19:50.92 Intake.select :close @09:19:51.05 SSV.dial :exhaust @09:19:51.84 Exhaust.select :open @09:19:51.95 SS.reconfigure SSexhale @09:19:51.99 SS.seek 0.00ml @09:20:01.15 Exhaust.select :close @09:20:01.24 SSV.dial CBV @09:20:01.26
CBV.dial SSV @09:20:02.05 Allowing 185s for 25.0ml of remaining 900.0ml @09:20:02.06 Intake.select :open @09:20:04.19 Intake @45.7psia, vacuum from 20.0 to 24.0psi @09:20:04.20 SS.reconfigure forSampling @09:20:04.24 SS.seek 25.00ml @09:20:19.24 Vacuum too high -- waiting up to 40s @09:20:19.51 Intake @45.8psia, vacuum from 20.0 to 24.0psi @09:20:19.52 SS.reconfigure forSampling @09:20:19.55 SS.seek 25.00ml @09:20:56.62 Vacuum too high -- waiting up to 40s @09:20:56.89 Intake @44.1psia, vacuum from 20.0 to 24.0psi @09:20:56.90 SS.reconfigure forSampling @09:20:56.94 SS.seek 25.00ml @09:20:57.86 Stroke completed -- waiting for pressure to equalize ... @09:20:58.50 Intake.select :close @09:20:58.63 SSV.dial :exhaust @09:20:59.42 Exhaust.select :open @09:20:59.52 SS.reconfigure SSexhale @09:20:59.56 SS.seek 0.00ml @09:21:08.37 Exhaust.select :close @09:21:08.46 SSV.dial CBV @09:21:08.48
CBV.dial SSV @09:21:09.28 Allowing 185s for 25.0ml of remaining 875.0ml @09:21:09.29 Intake.select :open @09:21:11.42 Intake @44.3psia, vacuum from 20.0 to 24.0psi @09:21:11.43 SS.reconfigure forSampling @09:21:11.47 SS.seek 25.00ml @09:23:01.63 Stroke completed -- waiting for pressure to equalize ... @09:23:02.86 Intake.select :close @09:23:03.00 SSV.dial :exhaust @09:23:03.80 Exhaust.select :open @09:23:03.89 SS.reconfigure SSexhale @09:23:03.93 SS.seek 0.00ml @09:23:13.07 Exhaust.select :close @09:23:13.16 SSV.dial CBV @09:23:13.18
CBV.dial SSV @09:23:13.97 Allowing 185s for 25.0ml of remaining 850.0ml @09:23:13.98 Intake.select :open @09:23:16.11 Intake @43.6psia, vacuum from 20.0 to 24.0psi @09:23:16.12 SS.reconfigure forSampling @09:23:16.16 SS.seek 25.00ml @09:25:06.51 Stroke completed -- waiting for pressure to equalize ... @09:25:08.46 Intake.select :close @09:25:08.59 SSV.dial :exhaust @09:25:09.37 Exhaust.select :open @09:25:09.46 SS.reconfigure SSexhale @09:25:09.50 SS.seek 0.00ml @09:25:18.66 Exhaust.select :close @09:25:18.75 SSV.dial CBV @09:25:18.77
CBV.dial SSV @09:25:19.55 Allowing 185s for 25.0ml of remaining 825.0ml @09:25:19.56 Intake.select :open @09:25:21.69 Intake @42.5psia, vacuum from 20.0 to 24.0psi @09:25:21.71 SS.reconfigure forSampling @09:25:21.74 SS.seek 25.00ml @09:26:05.24 Vacuum too high -- waiting up to 40s @09:26:05.73 SS.seek 25.00ml @09:26:13.36 Vacuum too high -- waiting up to 40s @09:26:13.83 Intake @42.9psia, vacuum from 20.0 to 24.0psi @09:26:13.84 SS.reconfigure forSampling @09:26:13.88 SS.seek 25.00ml @09:26:16.59 Vacuum too high -- waiting up to 40s @09:26:17.07 Intake @42.5psia, vacuum from 20.0 to 24.0psi @09:26:17.08 SS.reconfigure forSampling @09:26:17.11 SS.seek 25.00ml @09:26:19.93 Vacuum too high -- waiting up to 40s @09:26:20.41 Intake @42.8psia, vacuum from 20.0 to 24.0psi @09:26:20.42 SS.reconfigure forSampling @09:26:20.45 SS.seek 25.00ml @09:26:25.11 Vacuum too high -- waiting up to 40s @09:26:25.59 Intake @42.7psia, vacuum from 20.0 to 24.0psi @09:26:25.60 SS.reconfigure forSampling @09:26:25.63 SS.seek 25.00ml @09:26:28.31 Vacuum too high -- waiting up to 40s @09:26:28.79 Intake @42.4psia, vacuum from 20.0 to 24.0psi @09:26:28.80 SS.reconfigure forSampling @09:26:28.83 SS.seek 25.00ml @09:26:31.56 Vacuum too high -- waiting up to 40s @09:26:32.04 Intake @42.7psia, vacuum from 20.0 to 24.0psi @09:26:32.05 SS.reconfigure forSampling @09:26:32.08 SS.seek 25.00ml @09:26:34.74 Vacuum too high -- waiting up to 40s @09:26:35.57 Intake @43.1psia, vacuum from 20.0 to 24.0psi @09:26:35.58 SS.reconfigure forSampling @09:26:35.62 SS.seek 25.00ml @09:26:38.46 Vacuum too high -- waiting up to 40s @09:26:38.94 Intake @43.0psia, vacuum from 20.0 to 24.0psi @09:26:38.95 SS.reconfigure forSampling @09:26:38.99 SS.seek 25.00ml @09:26:41.40 Vacuum too high -- waiting up to 40s @09:26:41.88 Intake @43.1psia, vacuum from 20.0 to 24.0psi @09:26:41.89 SS.reconfigure forSampling @09:26:41.92 SS.seek 25.00ml @09:26:44.34 Vacuum too high -- waiting up to 40s @09:26:44.83 Intake @43.0psia, vacuum from 20.0 to 24.0psi @09:26:44.84 SS.reconfigure forSampling @09:26:44.87 SS.seek 25.00ml @09:26:46.87 Vacuum too high -- waiting up to 40s @09:26:47.36 Intake @42.5psia, vacuum from 20.0 to 24.0psi @09:26:47.37 SS.reconfigure forSampling @09:26:47.40 SS.seek 25.00ml @09:26:49.65 Vacuum too high -- waiting up to 40s @09:26:50.13 Intake @42.1psia, vacuum from 20.0 to 24.0psi @09:26:50.14 SS.reconfigure forSampling @09:26:50.17 SS.seek 25.00ml @09:26:52.24 Vacuum too high -- waiting up to 40s @09:26:52.71 Intake @42.1psia, vacuum from 20.0 to 24.0psi @09:26:52.72 SS.reconfigure forSampling @09:26:52.75 SS.seek 25.00ml @09:26:55.21 Vacuum too high -- waiting up to 40s @09:26:55.68 Intake @43.2psia, vacuum from 20.0 to 24.0psi @09:26:55.69 SS.reconfigure forSampling @09:26:55.72 SS.seek 25.00ml @09:26:57.56 Vacuum too high -- waiting up to 40s @09:26:58.03 Intake @42.6psia, vacuum from 20.0 to 24.0psi @09:26:58.04 SS.reconfigure forSampling @09:26:58.08 SS.seek 25.00ml @09:27:00.34 Vacuum too high -- waiting up to 40s @09:27:00.81 Intake @42.5psia, vacuum from 20.0 to 24.0psi @09:27:00.82 SS.reconfigure forSampling @09:27:00.85 SS.seek 25.00ml @09:27:02.80 Vacuum too high -- waiting up to 40s @09:27:03.28 Intake @42.4psia, vacuum from 20.0 to 24.0psi @09:27:03.29 SS.reconfigure forSampling @09:27:03.33 SS.seek 25.00ml @09:27:05.18 Vacuum too high -- waiting up to 40s @09:27:05.66 Intake @42.3psia, vacuum from 20.0 to 24.0psi @09:27:05.67 SS.reconfigure forSampling @09:27:05.70 SS.seek 25.00ml @09:27:07.62 Vacuum too high -- waiting up to 40s @09:27:08.11 SS.seek 25.00ml @09:27:10.24 Vacuum too high -- waiting up to 40s @09:27:10.71 Intake @43.8psia, vacuum from 20.0 to 24.0psi @09:27:10.72 SS.reconfigure forSampling @09:27:10.75 SS.seek 25.00ml @09:27:12.05 Vacuum too high -- waiting up to 40s @09:27:12.53 Intake @42.5psia, vacuum from 20.0 to 24.0psi @09:27:12.54 SS.reconfigure forSampling @09:27:12.57 SS.seek 25.00ml @09:27:14.49 Vacuum too high -- waiting up to 40s @09:27:14.97 Intake @42.2psia, vacuum from 20.0 to 24.0psi @09:27:14.98 SS.reconfigure forSampling @09:27:15.01 SS.seek 25.00ml @09:27:16.88 Vacuum too high -- waiting up to 40s @09:27:17.35 Intake @42.1psia, vacuum from 20.0 to 24.0psi @09:27:17.36 SS.reconfigure forSampling @09:27:17.39 SS.seek 25.00ml @09:27:19.24 Vacuum too high -- waiting up to 40s @09:27:19.71 Intake @41.8psia, vacuum from 20.0 to 24.0psi @09:27:19.72 SS.reconfigure forSampling @09:27:19.75 SS.seek 25.00ml @09:27:21.49 Vacuum too high -- waiting up to 40s @09:27:21.98 Intake @41.6psia, vacuum from 20.0 to 24.0psi @09:27:21.99 SS.reconfigure forSampling @09:27:22.03 SS.seek 25.00ml @09:27:23.93 Vacuum too high -- waiting up to 40s @09:27:24.41 Intake @42.0psia, vacuum from 20.0 to 24.0psi @09:27:24.42 SS.reconfigure forSampling @09:27:24.45 SS.seek 25.00ml @09:27:27.58 Vacuum too high -- waiting up to 40s @09:27:28.07 Intake @42.4psia, vacuum from 20.0 to 24.0psi @09:27:28.08 SS.reconfigure forSampling @09:27:28.12 SS.seek 25.00ml @09:27:29.99 Vacuum too high -- waiting up to 40s @09:27:30.47 Intake @42.7psia, vacuum from 20.0 to 24.0psi @09:27:30.49 SS.reconfigure forSampling @09:27:30.52 SS.seek 25.00ml @09:27:32.49 Vacuum too high -- waiting up to 40s @09:27:32.97 Intake @42.4psia, vacuum from 20.0 to 24.0psi @09:27:32.98 SS.reconfigure forSampling @09:27:33.02 SS.seek 25.00ml @09:27:34.71 Vacuum too high -- waiting up to 40s @09:27:35.19 Intake @41.8psia, vacuum from 20.0 to 24.0psi @09:27:35.20 SS.reconfigure forSampling @09:27:35.23 SS.seek 25.00ml @09:27:36.20 Stroke completed -- waiting for pressure to equalize ... @09:27:38.60 Intake.select :close @09:27:38.73 SSV.dial :exhaust @09:27:39.53 Exhaust.select :open @09:27:39.63 SS.reconfigure SSexhale @09:27:39.67 SS.seek 0.00ml @09:27:48.50 Exhaust.select :close @09:27:48.59 SSV.dial CBV @09:27:48.61
CBV.dial SSV @09:27:49.41 Allowing 185s for 25.0ml of remaining 800.0ml @09:27:49.42 Intake.select :open @09:27:51.55 Intake @42.1psia, vacuum from 20.0 to 24.0psi @09:27:51.56 SS.reconfigure forSampling @09:27:51.60 SS.seek 25.00ml @09:27:54.79 Vacuum too high -- waiting up to 40s @09:27:55.27 Intake @42.4psia, vacuum from 20.0 to 24.0psi @09:27:55.28 SS.reconfigure forSampling @09:27:55.31 SS.seek 25.00ml @09:27:56.93 Vacuum too high -- waiting up to 40s @09:27:57.41 Intake @41.8psia, vacuum from 20.0 to 24.0psi @09:27:57.42 SS.reconfigure forSampling @09:27:57.45 SS.seek 25.00ml @09:27:59.46 Vacuum too high -- waiting up to 40s @09:28:00.15 Intake @41.8psia, vacuum from 20.0 to 24.0psi @09:28:00.16 SS.reconfigure forSampling @09:28:00.19 SS.seek 25.00ml @09:28:02.33 Vacuum too high -- waiting up to 40s @09:28:02.81 Intake @42.6psia, vacuum from 20.0 to 24.0psi @09:28:02.82 SS.reconfigure forSampling @09:28:02.85 SS.seek 25.00ml @09:28:04.43 Vacuum too high -- waiting up to 40s @09:28:04.90 Intake @42.4psia, vacuum from 20.0 to 24.0psi @09:28:04.91 SS.reconfigure forSampling @09:28:04.94 SS.seek 25.00ml @09:28:06.55 Vacuum too high -- waiting up to 40s @09:28:07.03 Intake @41.8psia, vacuum from 20.0 to 24.0psi @09:28:07.04 SS.reconfigure forSampling @09:28:07.08 SS.seek 25.00ml @09:28:08.74 Vacuum too high -- waiting up to 40s @09:28:09.22 Intake @41.7psia, vacuum from 20.0 to 24.0psi @09:28:09.23 SS.reconfigure forSampling @09:28:09.27 SS.seek 25.00ml @09:28:11.08 Vacuum too high -- waiting up to 40s @09:28:11.56 Intake @41.7psia, vacuum from 20.0 to 24.0psi @09:28:11.57 SS.reconfigure forSampling @09:28:11.61 SS.seek 25.00ml @09:28:13.24 Vacuum too high -- waiting up to 40s @09:28:13.72 Intake @41.9psia, vacuum from 20.0 to 24.0psi @09:28:13.73 SS.reconfigure forSampling @09:28:13.77 SS.seek 25.00ml @09:28:15.71 Vacuum too high -- waiting up to 40s @09:28:16.19 Intake @42.6psia, vacuum from 20.0 to 24.0psi @09:28:16.20 SS.reconfigure forSampling @09:28:16.23 SS.seek 25.00ml @09:28:17.61 Vacuum too high -- waiting up to 40s @09:28:18.09 Intake @42.5psia, vacuum from 20.0 to 24.0psi @09:28:18.10 SS.reconfigure forSampling @09:28:18.13 SS.seek 25.00ml @09:28:19.71 Vacuum too high -- waiting up to 40s @09:28:20.20 Intake @42.2psia, vacuum from 20.0 to 24.0psi @09:28:20.21 SS.reconfigure forSampling @09:28:20.24 SS.seek 25.00ml @09:28:21.93 Vacuum too high -- waiting up to 40s @09:28:22.82 Intake @41.8psia, vacuum from 20.0 to 24.0psi @09:28:22.83 SS.reconfigure forSampling @09:28:22.86 SS.seek 25.00ml @09:28:25.08 Vacuum too high -- waiting up to 40s @09:28:25.78 Intake @42.4psia, vacuum from 20.0 to 24.0psi @09:28:25.79 SS.reconfigure forSampling @09:28:25.83 SS.seek 25.00ml @09:28:27.68 Vacuum too high -- waiting up to 40s @09:28:28.16 Intake @42.5psia, vacuum from 20.0 to 24.0psi @09:28:28.17 SS.reconfigure forSampling @09:28:28.20 SS.seek 25.00ml @09:28:29.74 Vacuum too high -- waiting up to 40s @09:28:30.22 Intake @42.6psia, vacuum from 20.0 to 24.0psi @09:28:30.23 SS.reconfigure forSampling @09:28:30.26 SS.seek 25.00ml @09:28:31.80 Vacuum too high -- waiting up to 40s @09:28:32.28 Intake @42.2psia, vacuum from 20.0 to 24.0psi @09:28:32.29 SS.reconfigure forSampling @09:28:32.32 SS.seek 25.00ml @09:28:33.96 Vacuum too high -- waiting up to 40s @09:28:34.44 Intake @41.8psia, vacuum from 20.0 to 24.0psi @09:28:34.45 SS.reconfigure forSampling @09:28:34.48 SS.seek 25.00ml @09:28:36.08 Vacuum too high -- waiting up to 40s @09:28:36.77 Intake @41.9psia, vacuum from 20.0 to 24.0psi @09:28:36.78 SS.reconfigure forSampling @09:28:36.82 SS.seek 25.00ml @09:28:38.64 Vacuum too high -- waiting up to 40s @09:28:39.33 Intake @42.6psia, vacuum from 20.0 to 24.0psi @09:28:39.34 SS.reconfigure forSampling @09:28:39.37 SS.seek 25.00ml @09:28:41.11 Vacuum too high -- waiting up to 40s @09:28:41.58 Intake @42.7psia, vacuum from 20.0 to 24.0psi @09:28:41.59 SS.reconfigure forSampling @09:28:41.62 SS.seek 25.00ml @09:28:43.05 Vacuum too high -- waiting up to 40s @09:28:43.53 Intake @42.4psia, vacuum from 20.0 to 24.0psi @09:28:43.54 SS.reconfigure forSampling @09:28:43.57 SS.seek 25.00ml @09:28:45.08 Vacuum too high -- waiting up to 40s @09:28:45.55 Intake @41.7psia, vacuum from 20.0 to 24.0psi @09:28:45.56 SS.reconfigure forSampling @09:28:45.59 SS.seek 25.00ml @09:28:47.63 Vacuum too high -- waiting up to 40s @09:28:47.90 Intake @41.8psia, vacuum from 20.0 to 24.0psi @09:28:47.91 SS.reconfigure forSampling @09:28:47.94 SS.seek 25.00ml @09:28:49.74 Vacuum too high -- waiting up to 40s @09:28:50.42 Intake @41.8psia, vacuum from 20.0 to 24.0psi @09:28:50.43 SS.reconfigure forSampling @09:28:50.46 SS.seek 25.00ml @09:28:52.24 Vacuum too high -- waiting up to 40s @09:28:52.93 Intake @42.2psia, vacuum from 20.0 to 24.0psi @09:28:52.94 SS.reconfigure forSampling @09:28:52.97 SS.seek 25.00ml @09:28:54.71 Vacuum too high -- waiting up to 40s @09:28:55.41 Intake @42.3psia, vacuum from 20.0 to 24.0psi @09:28:55.42 SS.reconfigure forSampling @09:28:55.45 SS.seek 25.00ml @09:28:57.05 Vacuum too high -- waiting up to 40s @09:28:57.53 Intake @42.3psia, vacuum from 20.0 to 24.0psi @09:28:57.54 SS.reconfigure forSampling @09:28:57.58 SS.seek 25.00ml @09:28:59.08 Vacuum too high -- waiting up to 40s @09:28:59.78 Intake @42.0psia, vacuum from 20.0 to 24.0psi @09:28:59.79 SS.reconfigure forSampling @09:28:59.82 SS.seek 25.00ml @09:29:01.52 Vacuum too high -- waiting up to 40s @09:29:02.21 Intake @41.8psia, vacuum from 20.0 to 24.0psi @09:29:02.22 SS.reconfigure forSampling @09:29:02.26 SS.seek 25.00ml @09:29:04.11 Vacuum too high -- waiting up to 40s @09:29:05.21 Intake @42.1psia, vacuum from 20.0 to 24.0psi @09:29:05.22 SS.reconfigure forSampling @09:29:05.25 SS.seek 25.00ml @09:29:07.27 Vacuum too high -- waiting up to 40s @09:29:07.97 Intake @42.3psia, vacuum from 20.0 to 24.0psi @09:29:07.98 SS.reconfigure forSampling @09:29:08.02 SS.seek 25.00ml @09:29:09.83 Vacuum too high -- waiting up to 40s @09:29:10.53 Intake @41.9psia, vacuum from 20.0 to 24.0psi @09:29:10.54 SS.reconfigure forSampling @09:29:10.58 SS.seek 25.00ml @09:29:12.36 Vacuum too high -- waiting up to 40s @09:29:13.05 Intake @41.9psia, vacuum from 20.0 to 24.0psi @09:29:13.06 SS.reconfigure forSampling @09:29:13.10 SS.seek 25.00ml @09:29:14.67 Vacuum too high -- waiting up to 40s @09:29:15.36 Intake @42.1psia, vacuum from 20.0 to 24.0psi @09:29:15.37 SS.reconfigure forSampling @09:29:15.40 SS.seek 25.00ml @09:29:17.17 Vacuum too high -- waiting up to 40s @09:29:17.87 Intake @42.9psia, vacuum from 20.0 to 24.0psi @09:29:17.88 SS.reconfigure forSampling @09:29:17.91 SS.seek 25.00ml @09:29:19.55 Vacuum too high -- waiting up to 40s @09:29:20.25 Intake @41.9psia, vacuum from 20.0 to 24.0psi @09:29:20.26 SS.reconfigure forSampling @09:29:20.29 SS.seek 25.00ml @09:29:21.95 Vacuum too high -- waiting up to 40s @09:29:22.64 Intake @41.6psia, vacuum from 20.0 to 24.0psi @09:29:22.65 SS.reconfigure forSampling @09:29:22.69 SS.seek 25.00ml @09:29:24.36 Vacuum too high -- waiting up to 40s @09:29:25.06 SS.seek 25.00ml @09:29:26.70 Vacuum too high -- waiting up to 40s @09:29:27.40 SS.seek 25.00ml @09:29:29.11 Vacuum too high -- waiting up to 40s @09:29:29.81 Intake @42.4psia, vacuum from 20.0 to 24.0psi @09:29:29.82 SS.reconfigure forSampling @09:29:29.85 SS.seek 25.00ml @09:29:31.52 Vacuum too high -- waiting up to 40s @09:29:32.22 Intake @42.8psia, vacuum from 20.0 to 24.0psi @09:29:32.23 SS.reconfigure forSampling @09:29:32.26 SS.seek 25.00ml @09:29:33.77 Vacuum too high -- waiting up to 40s @09:29:34.47 Intake @42.0psia, vacuum from 20.0 to 24.0psi @09:29:34.48 SS.reconfigure forSampling @09:29:34.51 SS.seek 25.00ml @09:29:36.14 Vacuum too high -- waiting up to 40s @09:29:36.61 Intake @41.7psia, vacuum from 20.0 to 24.0psi @09:29:36.62 SS.reconfigure forSampling @09:29:36.66 SS.seek 25.00ml @09:29:38.08 Vacuum too high -- waiting up to 40s @09:29:38.78 SS.seek 25.00ml @09:29:40.27 Vacuum too high -- waiting up to 40s @09:29:40.97 Intake @41.8psia, vacuum from 20.0 to 24.0psi @09:29:40.98 SS.reconfigure forSampling @09:29:41.01 SS.seek 25.00ml @09:29:42.55 Vacuum too high -- waiting up to 40s @09:29:43.24 Intake @42.3psia, vacuum from 20.0 to 24.0psi @09:29:43.25 SS.reconfigure forSampling @09:29:43.28 SS.seek 25.00ml @09:29:44.80 Vacuum too high -- waiting up to 40s @09:29:45.94 Intake @42.2psia, vacuum from 20.0 to 24.0psi @09:29:45.95 SS.reconfigure forSampling @09:29:45.99 SS.seek 25.00ml @09:29:47.92 Vacuum too high -- waiting up to 40s @09:29:48.40 Intake @41.9psia, vacuum from 20.0 to 24.0psi @09:29:48.41 SS.reconfigure forSampling @09:29:48.45 SS.seek 25.00ml @09:29:49.76 Vacuum too high -- waiting up to 40s @09:29:50.46 Intake @41.9psia, vacuum from 20.0 to 24.0psi @09:29:50.47 SS.reconfigure forSampling @09:29:50.51 SS.seek 25.00ml @09:29:52.08 Vacuum too high -- waiting up to 40s @09:29:52.77 Intake @42.2psia, vacuum from 20.0 to 24.0psi @09:29:52.78 SS.reconfigure forSampling @09:29:52.81 SS.seek 25.00ml @09:29:54.39 Vacuum too high -- waiting up to 40s @09:29:55.07 Intake @42.8psia, vacuum from 20.0 to 24.0psi @09:29:55.08 SS.reconfigure forSampling @09:29:55.11 SS.seek 25.00ml @09:29:56.48 Vacuum too high -- waiting up to 40s @09:29:56.95 Intake @42.1psia, vacuum from 20.0 to 24.0psi @09:29:56.96 SS.reconfigure forSampling @09:29:56.99 SS.seek 25.00ml @09:29:58.30 Vacuum too high -- waiting up to 40s @09:29:58.99 Intake @41.8psia, vacuum from 20.0 to 24.0psi @09:29:59.00 SS.reconfigure forSampling @09:29:59.03 SS.seek 25.00ml @09:30:00.64 Vacuum too high -- waiting up to 40s @09:30:01.12 Intake @41.8psia, vacuum from 20.0 to 24.0psi @09:30:01.13 SS.reconfigure forSampling @09:30:01.17 SS.seek 25.00ml @09:30:02.45 Vacuum too high -- waiting up to 40s @09:30:02.93 Intake @41.4psia, vacuum from 20.0 to 24.0psi @09:30:02.94 SS.reconfigure forSampling @09:30:02.97 SS.seek 25.00ml @09:30:04.33 Vacuum too high -- waiting up to 40s @09:30:05.03 Intake @42.3psia, vacuum from 20.0 to 24.0psi @09:30:05.04 SS.reconfigure forSampling @09:30:05.08 SS.seek 25.00ml @09:30:06.55 Vacuum too high -- waiting up to 40s @09:30:07.25 Intake @42.6psia, vacuum from 20.0 to 24.0psi @09:30:07.26 SS.reconfigure forSampling @09:30:07.30 SS.seek 25.00ml @09:30:08.80 Vacuum too high -- waiting up to 40s @09:30:09.26 Intake @41.9psia, vacuum from 20.0 to 24.0psi @09:30:09.27 SS.reconfigure forSampling @09:30:09.31 SS.seek 25.00ml @09:30:10.64 Vacuum too high -- waiting up to 40s @09:30:11.34 Intake @41.9psia, vacuum from 20.0 to 24.0psi @09:30:11.35 SS.reconfigure forSampling @09:30:11.38 SS.seek 25.00ml @09:30:12.86 Vacuum too high -- waiting up to 40s @09:30:13.56 SS.seek 25.00ml @09:30:14.92 Vacuum too high -- waiting up to 40s @09:30:15.40 Intake @42.3psia, vacuum from 20.0 to 24.0psi @09:30:15.41 SS.reconfigure forSampling @09:30:15.44 SS.seek 25.00ml @09:30:16.61 Vacuum too high -- waiting up to 40s @09:30:17.33 Intake @42.3psia, vacuum from 20.0 to 24.0psi @09:30:17.34 SS.reconfigure forSampling @09:30:17.37 SS.seek 25.00ml @09:30:18.73 Vacuum too high -- waiting up to 40s @09:30:19.42 Intake @42.1psia, vacuum from 20.0 to 24.0psi @09:30:19.43 SS.reconfigure forSampling @09:30:19.46 SS.seek 25.00ml @09:30:20.83 Vacuum too high -- waiting up to 40s @09:30:21.30 SS.seek 25.00ml @09:30:22.42 Vacuum too high -- waiting up to 40s @09:30:23.33 Intake @42.2psia, vacuum from 20.0 to 24.0psi @09:30:23.34 SS.reconfigure forSampling @09:30:23.38 SS.seek 25.00ml @09:30:25.01 Vacuum too high -- waiting up to 40s @09:30:25.69 Intake @42.7psia, vacuum from 20.0 to 24.0psi @09:30:25.70 SS.reconfigure forSampling @09:30:25.74 SS.seek 25.00ml @09:30:27.26 Vacuum too high -- waiting up to 40s @09:30:27.95 Intake @42.5psia, vacuum from 20.0 to 24.0psi @09:30:27.96 SS.reconfigure forSampling @09:30:27.99 SS.seek 25.00ml @09:30:29.33 Vacuum too high -- waiting up to 40s @09:30:29.81 Intake @42.2psia, vacuum from 20.0 to 24.0psi @09:30:29.83 SS.reconfigure forSampling @09:30:29.86 SS.seek 25.00ml @09:30:31.11 Vacuum too high -- waiting up to 40s @09:30:31.80 Intake @42.5psia, vacuum from 20.0 to 24.0psi @09:30:31.81 SS.reconfigure forSampling @09:30:31.85 SS.seek 25.00ml @09:30:33.20 Vacuum too high -- waiting up to 40s @09:30:33.68 Intake @42.4psia, vacuum from 20.0 to 24.0psi @09:30:33.69 SS.reconfigure forSampling @09:30:33.72 SS.seek 25.00ml @09:30:34.86 Vacuum too high -- waiting up to 40s @09:30:35.33 Intake @41.9psia, vacuum from 20.0 to 24.0psi @09:30:35.34 SS.reconfigure forSampling @09:30:35.37 SS.seek 25.00ml @09:30:36.51 Vacuum too high -- waiting up to 40s @09:30:37.00 Intake @41.9psia, vacuum from 20.0 to 24.0psi @09:30:37.01 SS.reconfigure forSampling @09:30:37.05 SS.seek 25.00ml @09:30:38.20 Vacuum too high -- waiting up to 40s @09:30:38.91 SS.seek 25.00ml @09:30:40.20 Vacuum too high -- waiting up to 40s @09:30:40.67 Intake @41.9psia, vacuum from 20.0 to 24.0psi @09:30:40.68 SS.reconfigure forSampling @09:30:40.71 SS.seek 25.00ml @09:30:41.86 Vacuum too high -- waiting up to 40s @09:30:42.55 Intake @42.4psia, vacuum from 20.0 to 24.0psi @09:30:42.56 SS.reconfigure forSampling @09:30:42.59 SS.seek 25.00ml @09:30:43.89 Vacuum too high -- waiting up to 40s @09:30:44.58 Intake @42.9psia, vacuum from 20.0 to 24.0psi @09:30:44.59 SS.reconfigure forSampling @09:30:44.62 SS.seek 25.00ml @09:30:46.04 Vacuum too high -- waiting up to 40s @09:30:46.73 Intake @42.0psia, vacuum from 20.0 to 24.0psi @09:30:46.74 SS.reconfigure forSampling @09:30:46.78 SS.seek 25.00ml @09:30:48.20 Vacuum too high -- waiting up to 40s @09:30:48.89 Intake @42.8psia, vacuum from 20.0 to 24.0psi @09:30:48.90 SS.reconfigure forSampling @09:30:48.93 SS.seek 25.00ml @09:30:50.14 Vacuum too high -- waiting up to 40s @09:30:50.61 Intake @42.2psia, vacuum from 20.0 to 24.0psi @09:30:50.62 SS.reconfigure forSampling @09:30:50.65 SS.seek 25.00ml @09:30:51.79 Vacuum too high -- waiting up to 40s @09:30:52.27 Intake @41.8psia, vacuum from 20.0 to 24.0psi @09:30:52.28 SS.reconfigure forSampling @09:30:52.31 SS.seek 25.00ml @09:30:53.51 Vacuum too high -- waiting up to 40s @09:30:54.21 Intake @41.8psia, vacuum from 20.0 to 24.0psi @09:30:54.22 SS.reconfigure forSampling @09:30:54.25 SS.seek 25.00ml @09:30:54.43 SS.reconfigure SSconfig @09:30:54.46 SS.hold @09:30:54.58 Intake.select :close @09:30:54.72 ShallowSampler::Clogged in MAIN -- progress too slow after 224.5ml SSV.dial :exhaust @09:30:55.51 Exhaust.select :open @09:30:56.21 SS.reconfigure SSexhale @09:30:56.25 SS.seek 0.00ml @09:31:05.13 Exhaust.select :close @09:31:05.23 Sampled 224.5ml @09:31:05.24 collectSample: Complete @09:31:05.25 Evacuating Puck (bubblePt=55.0psi, delay=15.0s) Intake.select :close @09:31:05.30 CSV.dial :waste @09:31:05.33 CS.seek 0.000ml @09:31:05.41 SSV.dial CBV,avoiding => exhaust @09:31:05.43
CBV.dial SSV @09:31:05.47 CSV.dial :air @09:31:06.31 CS.seek 10.000ml @09:31:07.41
CTV.dial :air @09:31:08.18 delay 15 seconds @09:31:08.22 Sleepy.power :sampler,:OFF @09:31:23.18
SSV.dial :spit,avoiding => exhaust @09:31:24.51 CTV.dial CSV,avoiding => intake @09:31:24.53
SS.seek 0.00ml @09:31:24.62 SSV.dial CBV,avoiding => exhaust @09:31:30.99 CBV.dial between CSV and waste2b @09:31:31.92 CSV.dial CTV @09:31:31.94 CTV.dial CSV,avoiding => air @09:31:32.37
CS.reconfigure CSpressurize @09:31:32.40 CS.seek 0.000ml @09:31:54.80 BubblePoint: Intake @70.2psia, Outlet @17.2psia @09:31:54.81 SS.reconfigure SSconfig @09:31:54.84 SS.seek 10.00ml @09:32:00.64 delay 15 seconds @09:32:15.74 Evacuated: Intake @69.3psia, Outlet @-0.4psia @09:32:15.75 SSV.dial :spit,avoiding => exhaust @09:32:17.07 SS.reconfigure SSexhale @09:32:17.10 SS.seek 0.00ml @09:32:21.92 SS.reconfigure SSfast @09:32:21.96 SS.seek 5.00ml @09:32:25.06 SSV.dial CBV,avoiding => exhaust @09:32:25.08
CBV.dial SSV @09:32:26.40 CTV.dial :air,avoiding => intake @09:32:27.70 CSV.dial CTV @09:32:27.71
SS.reconfigure SSconfig @09:32:27.75 SS.seek 10.00ml @09:32:28.71 CSV.dial :air @09:32:29.21 CS.reconfigure CSfast @09:32:29.24 CS.seek 0.000ml @09:32:30.86
SSV.dial :spit,avoiding => exhaust @09:32:32.18 SS.reconfigure SSexhale @09:32:32.21 SS.seek 0.00ml @09:32:33.19 CS.coast @09:32:37.16
SS.reconfigure SSfast @09:32:37.20 SS.seek 5.00ml @09:32:40.35 SSV.dial CBV,avoiding => exhaust @09:32:40.37
CBV.dial SSV @09:32:42.67 SSV.dial :spit,avoiding => exhaust @09:32:43.98 SS.reconfigure SSconfig @09:32:44.01 SS.seek 0.00ml @09:32:47.08 SS.coast @09:32:47.11 Evacuating Puck (bubblePt=55.0psi, delay=15.0s) Intake.select :close @09:32:47.17 CSV.dial :waste @09:32:47.26
SSV.dial :exhaust @09:32:47.93 CS.seek 0.000ml @09:32:48.01 CSV.dial :air @09:32:48.09
Exhaust.select :open @09:32:48.19 SS.reconfigure SSexhale @09:32:48.23 SS.seek 0.00ml @09:32:48.59 Exhaust.select :close @09:32:48.68 SSV.dial CBV,avoiding => exhaust @09:32:48.70
CBV.dial SSV @09:32:48.82 CS.seek 10.000ml @09:32:50.59
CTV.dial :air @09:32:50.60 delay 15 seconds @09:33:05.60 SSV.dial :spit,avoiding => exhaust @09:33:06.93 CTV.dial CSV,avoiding => intake @09:33:06.95
SS.seek 0.00ml @09:33:07.05 SSV.dial CBV,avoiding => exhaust @09:33:12.86 CBV.dial between CSV and waste2b @09:33:13.80 CSV.dial between air and CTV @09:33:15.12 CS.seek 6.989ml @09:33:22.85 CTV.dial CSV,avoiding => air @09:33:22.86
CSV.dial CTV @09:33:23.24 CS.reconfigure CSpressurize @09:33:23.27 CS.seek 0.000ml @09:33:40.92 BubblePoint: Intake @75.0psia, Outlet @19.3psia SS.reconfigure SSconfig @09:33:40.96 SS.seek 10.00ml @09:33:46.86 delay 15 seconds @09:34:01.95 Evacuated: Intake @73.9psia, Outlet @-0.2psia SSV.dial :spit,avoiding => exhaust @09:34:03.27 SS.reconfigure SSexhale @09:34:03.30 SS.seek 0.00ml @09:34:08.26 SS.reconfigure SSfast @09:34:08.29 SS.seek 5.00ml @09:34:11.21 SSV.dial CBV,avoiding => exhaust @09:34:11.23
CBV.dial SSV @09:34:12.57 CTV.dial :air,avoiding => intake @09:34:14.43 CSV.dial CTV @09:34:14.44
SS.reconfigure SSconfig @09:34:14.48 SS.seek 10.00ml @09:34:15.44 CSV.dial :air @09:34:16.18 CS.reconfigure CSfast @09:34:16.21 CS.seek 0.000ml @09:34:17.62
SSV.dial :spit,avoiding => exhaust @09:34:17.74 CS.coast @09:34:18.93
SS.reconfigure SSexhale @09:34:18.96 SS.seek 0.00ml @09:34:23.96 SS.reconfigure SSfast @09:34:24.00 SS.seek 5.00ml @09:34:27.08 SSV.dial CBV,avoiding => exhaust @09:34:27.10
CBV.dial SSV @09:34:29.40 SSV.dial :spit,avoiding => exhaust @09:34:30.71 SS.reconfigure SSconfig @09:34:30.74 SS.seek 0.00ml @09:34:33.79 SS.coast @09:34:33.82 Lysing: Adding 1.5ml lysis CBV.dial between CSV and waste1,avoiding => DBV @09:34:35.28 CTV.dial CSV @09:34:36.54 CSV.dial CSR @09:34:37.24 CSR.select :lysis @09:34:37.34 CS.reconfigure CSconfig @09:34:37.38 CS.seek 1.000ml @09:34:40.71 delay 5 seconds @09:34:45.72 CSR.select :air @09:34:45.86 CS.seek 6.000ml @09:34:59.31 delay 2 seconds @09:35:01.32 CSV.dial :waste1 @09:35:02.58 CS.reconfigure CSfast @09:35:02.61 CS.seek empty @09:35:17.34 CSV.dial CSR @09:35:18.60 CSR.select :lysis @09:35:18.70 CS.reconfigure CSconfig @09:35:18.74 CS.seek 1.500ml @09:35:23.40 delay 5 seconds @09:35:28.41 CSR.select :air @09:35:28.55 CS.seek 4.500ml @09:35:37.02 delay 2 seconds @09:35:39.04 CSV.dial CTV @09:35:39.55 CS.seek 1.275ml @09:35:48.59 Heating sh1 puck from tube 2 in Collection Clamp to 85.0C for 5:00 @09:35:48.60 CH.seek 85.0C,8:00 @09:38:40.93 CH.hold 5:00,85.0C @09:43:41.05 Collection Puck Heater at 84.9C after lysing @09:43:41.06 CS.seek 0.000ml @09:43:45.11 CTV.dial between spare and air,avoiding => intake @09:43:46.09 CSV.dial :air @09:43:46.52 CS.seek 5.000ml @09:44:00.01 CSV.dial CTV @09:44:00.51 delay 2 seconds @09:44:02.52 CSV.dial :air @09:44:03.02 delay 2 seconds @09:44:05.03 CSV.dial CTV @09:44:05.52 delay 2 seconds @09:44:07.53 CSV.dial :air @09:44:08.02 delay 2 seconds @09:44:10.03 CS.seek 0.000ml @09:44:23.57 Recovering Lysate into Collection syringe @09:44:23.58 CSV.dial CBV @09:44:24.59 CBV.dial CSV @09:44:24.95 CS.seek 5.000ml @09:44:38.48 delay 5 seconds @09:44:43.50 CTV.dial :air @09:44:43.96 CS.seek 7.001ml @09:44:49.85 delay 5 seconds @09:44:54.87 CSV.dial :air2 @09:44:55.30 CS.seek 10.000ml @09:45:03.69 CSV.dial between air and CTV,avoiding => CBV @09:45:04.81 Lysing Complete @09:45:04.86 Storing sh1 puck in Collection Clamp to tube 1 @09:45:04.98 SC.reconfigure SCtight @09:45:05.02 SC.seek 1 @09:45:07.32 SC.reconfigure SCloose @09:45:07.49 Elbow.reconfigure TightElbow @09:45:07.52 Elbow.seek 1 @09:45:09.12 Forearm.reconfigure TightForearm @09:45:09.15 Forearm.seek 1 + 800 counts @09:45:10.16 SE.reconfigure SEpick @09:45:10.21 SE.seek beamBreak @09:45:11.50 SE.configure SEconfig @09:45:11.62 Elbow.reconfigure LimpElbow @09:45:11.65 Forearm.reconfigure LooseForearm @09:45:11.69 Forearm.seek 1 @09:45:11.93 Elbow.hold @09:45:11.99 Elbow.reconfigure TightElbow @09:45:12.07 SE.seek 17.05 Pucks below Top Plate @09:45:12.40 Forearm.coast @09:45:12.43 Forearm.seek 1 + 334 counts @09:45:12.66 Forearm.reconfigure TightForearm @09:45:12.70 Forearm.seek 1 + 800 counts @09:45:12.94 SE.seek 18.05 Pucks below Top Plate @09:45:13.45 Forearm.reconfigure LooseForearm @09:45:13.48 Forearm.seek retract @09:45:14.40 Elbow.seek CC @09:45:15.90 Elbow.reconfigure LooseElbow @09:45:15.99 Forearm.seek CC @09:45:16.95 CC.seek sealed @09:45:17.88 Hand.grip @09:45:19.34 CC.seek open @09:45:22.68 Forearm.seek retract @09:45:23.61 Elbow.reconfigure TightElbow @09:45:23.65 Elbow.seek 1 @09:45:25.25 Elbow.reconfigure LimpElbow @09:45:25.30 Forearm.seek 1 @09:45:26.30 Elbow.hold @09:45:26.35 Elbow.reconfigure TightElbow @09:45:26.39 Hand.release @09:45:27.17 Forearm.coast @09:45:27.20 Forearm.seek 1 + 343 counts @09:45:27.43 Forearm.reconfigure TightForearm @09:45:27.46 Forearm.seek 1 + 800 counts @09:45:27.74 SE.seek down @09:45:28.67 SE.coast @09:45:28.85
Forearm.reconfigure LooseForearm @09:45:28.88 Forearm.seek retract @09:45:29.78 SC.coast @09:45:29.81 Tranferring lysate to PRV @09:45:29.82 CSV.dial :air @09:45:30.12 CS.seek 10.000ml @09:45:30.17 CSV.dial between air and CTV @09:45:30.55 AIV.dial :bypass @09:45:30.62 Beginning samplePassC @09:45:30.63 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @09:45:30.77 Elbow.seek FlushPuck::Garage @09:45:32.57 Elbow.reconfigure LooseElbow @09:45:32.65 Forearm.seek garage @09:45:33.66 Hand.grip @09:45:34.43 Forearm.seek retract @09:45:35.42 Elbow.reconfigure TightElbow @09:45:35.46 Elbow.seek CC @09:45:38.28 Elbow.reconfigure LooseElbow @09:45:38.37 Forearm.reconfigure TightForearm @09:45:38.40 Forearm.seek CC @09:45:39.44 CC.seek closed @09:45:43.25 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 2.02 @09:45:43.30 Hand.release @09:45:44.13 Forearm.reconfigure LooseForearm @09:45:44.16 Forearm.seek retract @09:45:45.13 AWV.select :air @09:45:45.14 AIV.dial :bypass @09:45:45.15 PSV.dial PSR @09:45:46.42 PSR.select :flush @09:45:46.52 PS.seek 1.500ml @09:46:02.58 delay 5 seconds @09:46:07.60 PSV.dial :waste1 @09:46:08.03 PS.seek 0.000ml @09:46:24.08 PSV.dial PSR @09:46:24.52 PSR.select :air @09:46:24.62 PS.seek 2.500ml @09:46:50.96 CBV.dial PRV @09:46:50.97 PRV.dial CBV @09:46:51.73
PSV.dial :mixloop @09:46:52.72 CTV.dial :air,avoiding => intake @09:46:52.73 PTV.dial :PRVmixing @09:46:52.81 PS.seek 0.000ml @09:47:19.05 PSV.dial :air @09:47:19.49 PS.seek 2.500ml @09:47:45.80 PSV.dial :mixloop @09:47:46.24 PS.seek 0.000ml @09:48:12.51 PSV.dial :air @09:48:12.96 PS.seek 2.500ml @09:48:39.17 PSV.dial :mixloop @09:48:39.62 PS.seek 0.000ml @09:49:05.82 Garaging FlushPuck in Collection Clamp @09:49:05.88 Elbow.reconfigure TightElbow @09:49:05.91 Elbow.seek CC @09:49:06.11 Elbow.reconfigure LooseElbow @09:49:06.84 Forearm.seek CC @09:49:07.82 CC.seek sealed @09:49:08.75 Hand.grip @09:49:09.46 CC.seek open @09:49:12.81 Forearm.seek retract @09:49:13.74 Elbow.reconfigure TightElbow @09:49:13.78 Elbow.seek garage @09:49:16.56 Elbow.reconfigure LooseElbow @09:49:16.65 Forearm.reconfigure TightForearm @09:49:16.68 Forearm.seek garage @09:49:17.84 Hand.release @09:49:18.62 Forearm.reconfigure LooseForearm @09:49:18.65 Forearm.seek retract @09:49:19.68 Connecting PS to CS to transfer sample @09:49:19.69 CBV.dial AIV @09:49:19.70 AIV.dial :bypass @09:49:19.71 PSV.dial :mixloop @09:49:19.72 PTV.dial :PRVmixing @09:49:19.73 PRV.dial AIV CTV.dial :air,avoiding => intake @09:49:19.74 Loading lyfil puck from tube 2 into Collection Clamp @09:49:19.92 SC.reconfigure SCtight @09:49:19.95 SC.seek 2 @09:49:22.19 SC.reconfigure SCloose @09:49:22.36 Elbow.reconfigure TightElbow @09:49:22.40 Elbow.seek 2 @09:49:24.22 Forearm.reconfigure TightForearm @09:49:24.25 Forearm.seek 2 + 800 counts @09:49:25.37 SE.reconfigure SEpick @09:49:25.42 SE.seek beamBreak @09:49:28.13 SE.configure SEconfig @09:49:28.25 Elbow.seek 2 @09:49:28.39 Forearm.seek 2 + 300 counts @09:49:28.59 Forearm.reconfigure LooseForearm @09:49:28.63 Hand.grip @09:49:29.34 SE.seek down @09:49:29.48
Forearm.seek retract @09:49:30.44 Elbow.seek CC @09:49:31.89 SE.coast @09:49:32.02
Elbow.reconfigure LooseElbow @09:49:32.11 Forearm.reconfigure TightForearm @09:49:32.14 Forearm.seek CC @09:49:33.26 CC.seek closed @09:49:37.01 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 2.00 @09:49:37.02 Hand.release @09:49:37.74 Forearm.reconfigure LooseForearm @09:49:37.77 Forearm.seek retract @09:49:38.69 SC.coast @09:49:38.72 CTV.dial CSV,avoiding => intake @09:49:40.01 CSV.dial CTV @09:49:40.30 CS.seek 5.000ml @09:49:53.78 delay 1:20 @09:51:13.79 CS.seek 2.500ml @09:51:20.93 delay 40 seconds @09:52:00.94 CS.seek 0.000ml @09:52:08.14 delay 1:00 @09:53:08.15 PSV.dial :air @09:53:08.71 PS.seek 2.500ml @09:53:34.88 PSV.dial :mixloop @09:53:35.30 delay 3 seconds @09:53:38.31 PSV.dial :air @09:53:38.75 delay 2 seconds @09:53:40.76 PSV.dial :mixloop @09:53:41.20 delay 3 seconds @09:53:44.21 PSV.dial :waste1 @09:53:45.48 PS.seek empty @09:54:11.72 CBV.dial :waste1 @09:54:12.17 delay 5 seconds @09:54:17.18 CBV.dial AIV @09:54:17.64 delay 10 seconds @09:54:27.65 CBV.dial :waste1 @09:54:28.12 delay 10 seconds @09:54:38.13 CBV.dial AIV @09:54:38.57 delay 10 seconds @09:54:48.58 CTV.dial CSV,avoiding => intake @09:54:48.59 CBV.dial between AIV and waste1 @09:54:48.92 CSV.dial :air @09:54:49.43 CS.seek 10.000ml @09:55:15.64 CSV.dial CTV @09:55:16.15 CS.seek 0.000ml @09:55:42.32 CBV.dial :waste1 @09:55:42.62 delay 10 seconds @09:55:53.26 CTV.dial :air,avoiding => intake @09:55:54.59 Storing lyfil puck in Collection Clamp to tube 1 @09:55:54.72 SC.reconfigure SCtight @09:55:54.75 SC.seek 1 @09:55:57.01 SC.reconfigure SCloose @09:55:57.19 Elbow.reconfigure TightElbow @09:55:57.22 Elbow.seek 1 @09:55:58.85 Forearm.reconfigure TightForearm @09:55:58.88 Forearm.seek 1 + 800 counts @09:55:59.90 SE.reconfigure SEpick @09:55:59.95 SE.seek beamBreak @09:56:01.13 SE.configure SEconfig @09:56:01.25 Elbow.reconfigure LimpElbow @09:56:01.28 Forearm.reconfigure LooseForearm @09:56:01.32 Forearm.seek 1 @09:56:01.52 Elbow.hold @09:56:01.57 Elbow.reconfigure TightElbow @09:56:02.43 SE.seek 18.05 Pucks below Top Plate @09:56:02.79 Forearm.coast @09:56:02.82 Forearm.seek 1 + 337 counts @09:56:03.06 Forearm.reconfigure TightForearm @09:56:03.09 Forearm.seek 1 + 800 counts @09:56:03.38 SE.seek 19.05 Pucks below Top Plate @09:56:04.16 Forearm.reconfigure LooseForearm @09:56:04.19 Forearm.seek retract @09:56:05.11 Elbow.seek CC @09:56:06.70 Elbow.reconfigure LooseElbow @09:56:06.79 Forearm.seek CC @09:56:07.75 CC.seek sealed @09:56:08.70 Hand.grip @09:56:09.44 CC.seek open @09:56:12.76 Forearm.seek retract @09:56:13.74 Elbow.reconfigure TightElbow @09:56:13.78 Elbow.seek 1 @09:56:15.38 Elbow.reconfigure LimpElbow @09:56:15.42 Forearm.seek 1 @09:56:16.43 Elbow.hold @09:56:16.48 Elbow.reconfigure TightElbow @09:56:16.51 Hand.release @09:56:17.27 Forearm.coast @09:56:17.30 Forearm.seek 1 + 327 counts @09:56:17.53 Forearm.reconfigure TightForearm @09:56:17.56 Forearm.seek 1 + 800 counts @09:56:17.85 SE.seek down @09:56:18.71 SE.coast @09:56:18.96
Forearm.reconfigure LooseForearm @09:56:19.00 Forearm.seek retract @09:56:19.94 SC.coast @09:56:19.97 PSV.dial :air @09:56:21.01 PS.seek 0.499ml @09:56:26.87 delay 3 seconds @09:56:29.88 PSV.dial :mixloop @09:56:30.32 PRV.dial :air @09:56:30.78 delay 3 seconds @09:56:33.79 PRV.dial CBV @09:56:34.24 CBV.dial AIV @09:56:34.69 PRV.dial between CBV and air @09:56:35.01 PRV.dial :air @09:56:35.34 PS.reconfigure PSslow @09:56:35.37 PS.seek 0.599ml @09:56:37.96 PRV.dial :waste2 @09:56:38.69 PS.reconfigure PSconfig @09:56:38.72 PS.seek empty @09:56:45.68 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @09:56:45.83 Elbow.seek FlushPuck::Garage @09:56:47.58 Elbow.reconfigure LooseElbow @09:56:47.67 Forearm.seek garage @09:56:48.71 Hand.grip @09:56:49.52 Forearm.seek retract @09:56:50.53 Elbow.reconfigure TightElbow @09:56:50.57 Elbow.seek CC @09:56:53.31 Elbow.reconfigure LooseElbow @09:56:54.05 Forearm.reconfigure TightForearm @09:56:54.09 Forearm.seek CC @09:56:55.18 CC.seek closed @09:56:58.91 Collection Clamp onSpring/offSpring for 5/22 samples, on/off torque 2.01 @09:56:58.96 Hand.release @09:56:59.73 Forearm.reconfigure LooseForearm @09:56:59.76 Forearm.seek retract @09:57:00.80 Clearing PML for modified lysate @09:57:00.81 CTV.dial :air,avoiding => intake PRV.dial :waste2 @09:57:00.82 PTV.dial :PRV,avoiding => puck @09:57:01.53 delay 2 seconds @09:57:03.53 PTV.dial :PRVmixing,avoiding => puck @09:57:04.26 delay 2 seconds @09:57:06.27 PSV.dial :PML @09:57:06.28 delay 2 seconds @09:57:08.28 PRV.dial :air @09:57:09.01 delay 2 seconds @09:57:11.07 PS.seek 1.499ml @09:57:27.21 delay 5 seconds @09:57:32.22 PS.seek 2.500ml @09:57:43.27 PSV.dial :air @09:57:43.73 PS.seek 0.000ml @09:58:09.96 mixing lysate with speDil @09:58:09.97 PSV.dial :PML @09:58:10.41 CBV.dial :PRV @09:58:10.42 PRV.dial :air @09:58:10.43 delay 2 seconds @09:58:12.44 PRV.dial :speDil @09:58:12.88 delay 2 seconds @09:58:14.94 PS.reconfigure PSslow1 @09:58:14.97 PS.seek 0.225ml @09:58:38.28 delay 5 seconds @09:58:43.29 PRV.dial CBV @09:58:44.01 delay 2 seconds @09:58:46.07 PS.seek 0.475ml @09:59:11.78 delay 5 seconds @09:59:16.79 PRV.dial :air @09:59:17.25 delay 2 seconds @09:59:19.26 PS.seek 0.925ml @10:00:05.71 delay 10 seconds @10:00:15.72 PRV.dial CBV @10:00:16.17 CTV.dial :air,avoiding => intake @10:00:16.18 PSV.dial :air @10:00:16.63 PS.reconfigure PSconfig @10:00:16.66 PS.seek 2.500ml @10:00:33.53 delay 3 seconds @10:00:36.54 PSV.dial PTV @10:00:37.26 PTV.dial :PRV,avoiding => puck @10:00:37.99 PS.seek 0.000ml @10:01:04.24 PSV.dial :air @10:01:04.97 PS.seek 2.500ml @10:01:31.15 delay 3 seconds @10:01:34.17 PSV.dial PTV @10:01:34.88 PTV.dial :PRV,avoiding => puck @10:01:34.90 PS.seek 0.000ml @10:02:01.21 Pushing modified lysate into AIV Injection Loop @10:02:01.22 CBV.dial AIV @10:02:01.23 AIV.dial :bypass @10:02:01.24 PRV.dial CBV @10:02:01.25 PSV.dial :air @10:02:01.97 PS.seek 2.500ml @10:02:28.18 delay 3 seconds @10:02:31.20 PSV.dial :PML @10:02:31.63 PTV.dial :PRVmixing,avoiding => puck @10:02:32.40 PS.reconfigure PSslow1 @10:02:32.43 PS.seek 1.850ml @10:03:38.86 delay 5 seconds @10:03:43.87 AIV.dial :sample @10:03:44.40 AWV.select :waste @10:03:44.56 PS.reconfigure PSslow2 @10:03:44.59 PS.seek 1.310ml @10:05:34.61 delay 15 seconds @10:05:49.62 AIV.dial :bypass @10:05:50.17 PS.reconfigure PSconfig @10:05:50.20 PS.seek 0.000ml @10:06:04.42 PSV.dial :air @10:06:04.87 PS.seek 2.500ml @10:06:31.04 PSV.dial :PML @10:06:31.49 PS.seek 0.000ml @10:06:57.79 AIV.dial :coilToMFB @10:06:57.80 AWV.select :air @10:06:57.94 Extracting Nucleic Acid from AIV @10:06:57.96
Beginning samplePassD @10:06:57.97 SPE.hold 2:46:40,55.0C @10:06:57.99 ASV.dial :SPEdelivery @10:06:58.12
AWV.select :air AIV.dial :bypass @10:06:58.13 PSV.dial PSR @10:06:58.54 delay 20 seconds @10:06:59.17
PSR.select :killMFB @10:06:59.27 PS.seek 1.500ml @10:07:15.29 delay 5 seconds @10:07:18.55 primeLysateINT @10:07:18.56 AWV.select :air @10:07:18.57 AIV.dial :coilToMFB ATV.dial :AIV @10:07:20.30
PSV.dial :waste1 @10:07:20.54 AS.reconfigure ASslow1 @10:07:20.57 AS.seek 0.239ml @10:07:20.75
PS.seek 0.000ml @10:07:36.85 PSV.dial PSR @10:07:37.31 PSR.select :air @10:07:37.41 PS.seek 2.500ml @10:08:03.66 PRV.dial CBV @10:08:03.68
CBV.dial PRV @10:08:03.69 PSV.dial :mixloop @10:08:04.73 CTV.dial :air,avoiding => intake @10:08:04.74 PTV.dial :PRVmixing @10:08:04.76 PS.seek 0.000ml @10:08:09.83 delay 10 seconds @10:08:19.84 ATV.dial :air2 @10:08:20.43 AS.seek 0.269ml @10:08:26.80 ATV.dial :waste2 @10:08:27.15 AS.reconfigure ASnorm @10:08:27.18 AS.seek empty @10:08:31.07
PSV.dial PSR @10:08:32.23 PSR.select :flush @10:08:32.33 PS.seek 1.500ml @10:08:41.36 Lysate primed! @10:08:41.37 AFV.select :holdingCoil @10:08:41.38 ATV.dial :air2 @10:08:41.77 AS.reconfigure ASslow1 @10:08:41.81 AS.seek 0.069ml @10:08:48.38
delay 5 seconds @10:08:53.39 PSV.dial :waste1 @10:08:53.86 PS.seek 0.000ml @10:08:56.41 delay 3 seconds @10:08:59.42 ATV.dial :AIV @10:09:00.10 AS.seek 0.469ml @10:09:09.97
PSV.dial PSR @10:09:10.43 PSR.select :air @10:09:10.53 PS.seek 2.500ml @10:09:36.82 PRV.dial CBV @10:09:36.84
CBV.dial PRV @10:09:36.85 PSV.dial :mixloop @10:09:37.86 CTV.dial :air,avoiding => intake @10:09:37.87 PTV.dial :PRVmixing @10:09:37.89 PS.seek 0.000ml @10:10:04.19 PSV.dial PSR @10:10:05.33 PSR.select :flush @10:10:05.43 PS.seek 1.500ml @10:10:21.50 delay 5 seconds @10:10:21.92 delay 5 seconds @10:10:26.51
PSV.dial :waste1 @10:10:26.98 Solid Phase Extraction Heater at 52.2C @10:10:26.99 ATV.dial ASV @10:10:27.03
PS.seek 0.000ml @10:10:27.33 ASV.dial :SPEdelivery @10:10:27.39 AS.seek 0.041ml @10:10:43.13
PSV.dial PSR @10:10:43.58 PSR.select :air @10:10:43.68 PS.seek 2.500ml @10:11:09.94 PRV.dial CBV @10:11:09.96
CBV.dial PRV @10:11:09.97 PSV.dial :mixloop @10:11:10.97 CTV.dial :air,avoiding => intake @10:11:10.98 PTV.dial :PRVmixing @10:11:11.00 PS.seek 0.000ml @10:11:37.28 PSV.dial PSR @10:11:38.30 PSR.select :flush @10:11:38.40 PS.seek 1.500ml @10:11:54.50 delay 5 seconds @10:11:54.92 delay 10 seconds @10:11:59.51
PSV.dial :waste1 @10:11:59.95 PS.seek 0.000ml @10:12:04.93 rinseLysateLineINT @10:12:04.94 AFV.select :carrierSolution @10:12:05.03 delay 2 seconds @10:12:07.04 AS.reconfigure ASconfig @10:12:07.07 AS.seek 1.000ml @10:12:16.06
PSV.dial PSR @10:12:16.50 PSR.select :air @10:12:16.60 PS.seek 2.500ml @10:12:42.90 PRV.dial CBV @10:12:42.92
CBV.dial PRV @10:12:42.93 PSV.dial :mixloop @10:12:43.93 CTV.dial :air,avoiding => intake @10:12:43.94 PTV.dial :PRVmixing @10:12:43.95 PS.reconfigure PSslow @10:12:43.99 PS.seek 1.200ml @10:13:03.23 delay 1 second @10:13:04.24 AFV.select :holdingCoil @10:13:04.33 delay 1 second @10:13:05.34 ATV.dial :AIV @10:13:05.68 AIV.dial :coilToMFB @10:13:05.69 AWV.select :waste @10:13:05.78 AS.reconfigure ASslow0 @10:13:05.82 AS.seek 0.000ml @10:13:10.96
PSV.dial :waste1 @10:13:12.27 PS.reconfigure PSconfig @10:13:12.30 PS.seek 0.000ml @10:13:25.43 PSV.dial :air @10:13:26.46 PS.seek 2.500ml @10:13:52.65 PSV.dial :mixloop @10:13:53.10 PS.reconfigure PSslow @10:13:53.13 PS.seek 0.000ml @10:14:44.40 PSV.dial :air @10:14:44.97 PS.reconfigure PSconfig @10:14:45.00 PS.seek 2.500ml @10:14:48.13 ATV.dial :air2 @10:14:48.70 AS.reconfigure ASslow1 @10:14:48.73 AS.seek 0.050ml @10:14:59.22 ATV.dial :AIV @10:14:59.80 AS.seek 0.010ml @10:15:08.75 ATV.dial :waste @10:15:10.05 AS.seek empty @10:15:11.27
PSV.dial :mixloop @10:15:11.70 PS.reconfigure PSslow @10:15:11.73 PS.seek 0.000ml @10:15:12.49 ethanol wash @10:15:12.50 ATV.dial :air2 @10:15:13.44 AS.reconfigure ASslow2 @10:15:13.47 AS.seek 0.009ml @10:15:23.98 delay 3 seconds @10:15:26.99 Pulling 0.4ml of Ethanol Wash @10:15:27.00 ATV.dial :ethanol @10:15:27.56 AS.reconfigure ASslow1 @10:15:27.59 AS.seek 0.409ml @10:16:03.06
Cleaning Collection Syringe @10:16:03.07 CSV.dial CSR @10:16:03.49 CSR.select :flush @10:16:03.59 CS.seek 4.000ml @10:16:14.54 delay 5 seconds @10:16:19.55 CSR.select :air @10:16:19.64 CS.seek 10.000ml @10:16:35.60 CSV.dial CTV @10:16:35.62
CTV.dial CSV @10:16:36.94 CBV.dial :waste1 @10:16:37.40 CS.seek 0.000ml @10:16:49.49 delay 3 seconds @10:16:52.50 ATV.dial :air1 @10:16:52.92 delay 3 seconds @10:16:55.98 AS.seek 0.434ml @10:17:01.32 delay 3 seconds @10:17:03.53
CSV.dial CSR @10:17:04.03 CSR.select :flush @10:17:04.13 CS.seek 4.000ml @10:17:04.33 ATV.dial :ethanol @10:17:04.72 AS.seek 0.419ml @10:17:08.01 delay 3 seconds @10:17:11.02 ATV.dial ASV @10:17:12.26 AS.seek 0.092ml @10:17:15.13
delay 5 seconds @10:17:20.14 CSR.select :air @10:17:20.23 CS.seek 10.000ml @10:17:36.19 CSV.dial CTV @10:17:36.21
CTV.dial CSV @10:17:36.72 CBV.dial :waste1 @10:17:36.74 CS.seek 0.000ml @10:18:02.96 Garaging FlushPuck in Collection Clamp @10:18:03.01 Elbow.reconfigure TightElbow @10:18:03.04 Elbow.seek CC @10:18:03.14 Elbow.reconfigure LooseElbow @10:18:03.22 Forearm.seek CC @10:18:04.16 CC.seek sealed @10:18:05.09 Hand.grip @10:18:05.77 CC.seek open @10:18:09.06 Forearm.seek retract @10:18:10.00 Elbow.reconfigure TightElbow @10:18:10.04 Elbow.seek garage @10:18:12.84 Elbow.reconfigure LooseElbow @10:18:12.92 Forearm.reconfigure TightForearm @10:18:12.95 Forearm.seek garage @10:18:14.11 Hand.release @10:18:14.94 Forearm.reconfigure LooseForearm @10:18:14.97 Forearm.seek retract @10:18:16.03 Whole Cell Archive sampling 1000ml @10:18:16.05 Loading wcr puck from tube 2 into Collection Clamp @10:18:16.22 SC.reconfigure SCtight @10:18:16.25 SC.seek 2 @10:18:18.50 SC.reconfigure SCloose @10:18:18.68 Elbow.reconfigure TightElbow @10:18:18.71 Elbow.seek 2 @10:18:19.16 delay 3 seconds @10:18:20.53 Forearm.reconfigure TightForearm @10:18:20.56 Forearm.seek 2 + 800 counts @10:18:21.70 SE.reconfigure SEpick @10:18:21.75 SE.seek beamBreak @10:18:22.17 ATV.dial :waste2 @10:18:22.78 AS.reconfigure ASconfig @10:18:22.81 AS.seek empty @10:18:24.78 SE.configure SEconfig @10:18:24.91 Elbow.seek 2 @10:18:25.05 Forearm.seek 2 + 300 counts @10:18:25.29 Forearm.reconfigure LooseForearm @10:18:25.33 Hand.grip @10:18:26.03 SE.seek down @10:18:26.81 Forearm.seek retract @10:18:27.82 Elbow.seek CC @10:18:28.52 ATV.dial :air2 @10:18:28.71 SE.coast @10:18:28.88 AS.reconfigure ASslow1 @10:18:28.91 AS.seek 1.000ml @10:18:29.34 Elbow.reconfigure LooseElbow @10:18:29.42 Forearm.reconfigure TightForearm @10:18:29.45 Forearm.seek CC @10:18:30.55 CC.seek closed @10:18:34.33 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.97 @10:18:34.34 Hand.release @10:18:35.06 Forearm.reconfigure LooseForearm @10:18:35.09 Forearm.seek retract @10:18:36.03 SC.coast @10:18:36.06 collectSample 1000ml w/bubble point of 55psi @10:18:36.08 Priming 7ml Sleepy.power :sampler,:ON @10:18:36.09 Intake.select :close @10:18:36.10 Exhaust.select :close @10:18:36.11 SS.hold @10:18:36.26 Calibrating pressure and temperature sensors @10:18:36.28 CTV.dial :air @10:18:37.60 SSV.dial :spit,avoiding => exhaust @10:18:37.61 SS.reconfigure SSfast @10:18:37.64 SS.seek 5.00ml @10:18:40.36 SSV.dial CBV @10:18:40.37 CBV.dial SSV @10:18:41.79 delay 3.8 seconds @10:18:45.68 SSV.dial :spit @10:18:47.04 SS.seek 0.00ml @10:18:49.57 SSV.dial CBV @10:18:50.93 CTV.dial :intake @10:18:51.67 Waiting for external sampler to finish priming... @10:18:51.68 delayUntil "10:28:36.08" @10:21:53.33 delay 5 seconds @10:21:58.34 ATV.dial ASV @10:21:59.06 ASV.dial :SPEdelivery @10:21:59.08 AS.seek 0.020ml @10:25:19.29 delay 5 seconds @10:25:24.30 ATV.dial :air2 @10:25:25.07 AS.seek 0.520ml @10:27:07.30 delay 5 seconds @10:27:12.31 ATV.dial ASV @10:27:13.07 ASV.dial :wasteR @10:27:13.67 AS.seek 0.300ml @10:27:58.78 delay 20 seconds @10:28:18.79 AS.seek 0.070ml @10:28:36.08 SSV.dial CBV @10:28:36.10 CBV.dial SSV @10:28:36.11 Allowing 55s for 7.0ml of remaining 7.0ml @10:28:36.12 Intake.select :open @10:28:38.25 Intake @51.0psia, vacuum from 20.0 to 24.0psi @10:28:38.27 SS.reconfigure forSampling @10:28:38.30 SS.seek 7.00ml @10:28:43.95 Stroke completed -- waiting for pressure to equalize ... @10:28:44.59 Intake.select :close @10:28:44.72 SSV.dial :exhaust @10:28:45.49 Exhaust.select :open @10:28:45.59 SS.reconfigure SSexhale @10:28:45.63 SS.seek 0.00ml @10:28:49.43 Exhaust.select :close @10:28:49.54 Sampling 1000.0ml @10:28:49.55 Intake.select :close @10:28:49.56 Exhaust.select :close @10:28:49.57 SS.reconfigure SSconfig @10:28:49.60 SS.hold @10:28:49.76 Calibrating pressure sensors @10:28:49.77 CTV.dial :air @10:28:50.54 SSV.dial :spit,avoiding => exhaust @10:28:51.30 SS.reconfigure SSfast @10:28:51.33 SS.seek 5.00ml @10:28:54.10 SSV.dial CBV @10:28:54.12 CBV.dial SSV @10:28:55.49 delay 3.8 seconds @10:28:59.38 SSV.dial :spit @10:29:00.75 SS.seek 0.00ml @10:29:03.45 SSV.dial CBV @10:29:04.81 CTV.dial :intake @10:29:05.59 CBV.dial SSV @10:29:05.60 SSV.dial CBV @10:29:05.62 Allowing 185s for 25.0ml of remaining 1000.0ml @10:29:05.63 Intake.select :open @10:29:05.95 delay 20 seconds @10:29:07.76 Intake @49.9psia, vacuum from 20.0 to 24.0psi @10:29:07.78 SS.reconfigure forSampling @10:29:07.81 SS.seek 25.00ml @10:29:22.48 Stroke completed -- waiting for pressure to equalize ... @10:29:23.73 Intake.select :close @10:29:23.86 SSV.dial :exhaust @10:29:24.65 Exhaust.select :open @10:29:24.75 SS.reconfigure SSexhale @10:29:24.79 SS.seek 0.00ml @10:29:25.96 ATV.dial :waste2 @10:29:26.50 AS.reconfigure ASconfig @10:29:26.53 AS.seek empty @10:29:30.95 Elution @10:29:30.96 ATV.dial :air1 @10:29:31.91 AS.reconfigure ASslow1 @10:29:31.94 AS.seek 0.049ml @10:29:33.54 Exhaust.select :close @10:29:33.64 SSV.dial CBV @10:29:33.66 CBV.dial SSV @10:29:34.46 Allowing 185s for 25.0ml of remaining 975.0ml @10:29:34.47 Intake.select :open @10:29:36.60 Intake @48.8psia, vacuum from 20.0 to 24.0psi @10:29:36.62 SS.reconfigure forSampling @10:29:36.65 SS.seek 25.00ml @10:29:42.44 delay 3 seconds @10:29:45.45 ATV.dial :ElutionWater @10:29:45.76 Vacuum too high -- waiting up to 40s @10:29:46.09 AS.seek 0.109ml @10:29:46.12 Intake @41.7psia, vacuum from 20.0 to 24.0psi @10:29:46.14 SS.reconfigure forSampling @10:29:46.19 SS.seek 25.00ml @10:29:52.85 Stroke completed -- waiting for pressure to equalize ... @10:29:53.49 Intake.select :close @10:29:53.63 SSV.dial :exhaust @10:29:54.41 Exhaust.select :open @10:29:54.51 SS.reconfigure SSexhale @10:29:54.55 SS.seek 0.00ml @10:29:58.67 delay 3 seconds @10:30:01.68 ATV.dial ASV,via => air2 @10:30:03.47 ASV.dial :SPEelution @10:30:03.69 Exhaust.select :close @10:30:03.79 SSV.dial CBV @10:30:03.81 CBV.dial SSV @10:30:03.97 AFV.select :carrierSolution @10:30:04.12 AS.reconfigure ASnorm @10:30:04.15 AS.seek 0.759ml @10:30:04.61 Allowing 185s for 25.0ml of remaining 950.0ml @10:30:04.62 Intake.select :open @10:30:06.76 Intake @47.3psia, vacuum from 20.0 to 24.0psi @10:30:06.77 SS.reconfigure forSampling @10:30:06.81 SS.seek 25.00ml @10:30:13.51 Vacuum too high -- waiting up to 40s @10:30:13.77 Intake @42.5psia, vacuum from 20.0 to 24.0psi @10:30:13.78 SS.reconfigure forSampling @10:30:13.82 SS.seek 25.00ml @10:30:22.35 Vacuum too high -- waiting up to 40s @10:30:22.62 Intake @43.5psia, vacuum from 20.0 to 24.0psi @10:30:22.64 SS.reconfigure forSampling @10:30:22.67 SS.seek 25.00ml @10:30:25.52 Stroke completed -- waiting for pressure to equalize ... @10:30:26.16 Intake.select :close @10:30:26.29 SSV.dial :exhaust @10:30:27.08 Exhaust.select :open @10:30:27.18 SS.reconfigure SSexhale @10:30:27.22 SS.seek 0.00ml @10:30:36.32 Exhaust.select :close @10:30:36.42 SSV.dial CBV @10:30:36.44 CBV.dial SSV @10:30:37.24 Allowing 185s for 25.0ml of remaining 925.0ml @10:30:37.26 Intake.select :open @10:30:37.67 delay 3 seconds @10:30:39.39 Intake @47.0psia, vacuum from 20.0 to 24.0psi @10:30:39.41 SS.reconfigure forSampling @10:30:39.44 SS.seek 25.00ml @10:30:40.68 AFV.select :holdingCoil @10:30:40.82 AS.reconfigure ASslow1 @10:30:40.85 AS.seek 0.489ml @10:30:42.10 Vacuum too high -- waiting up to 40s @10:30:42.37 Intake @43.4psia, vacuum from 20.0 to 24.0psi @10:30:42.39 SS.reconfigure forSampling @10:30:42.42 SS.seek 25.00ml @10:30:49.22 Vacuum too high -- waiting up to 40s @10:30:49.70 Intake @46.1psia, vacuum from 20.0 to 24.0psi @10:30:49.72 SS.reconfigure forSampling @10:30:49.75 SS.seek 25.00ml @10:30:53.06 Vacuum too high -- waiting up to 40s @10:30:53.33 Intake @44.3psia, vacuum from 20.0 to 24.0psi @10:30:53.35 SS.reconfigure forSampling @10:30:53.38 SS.seek 25.00ml @10:31:06.16 Vacuum too high -- waiting up to 40s @10:31:06.65 Intake @45.9psia, vacuum from 20.0 to 24.0psi @10:31:06.66 SS.reconfigure forSampling @10:31:06.70 SS.seek 25.00ml @10:31:10.46 Stroke completed -- waiting for pressure to equalize ... @10:31:11.10 Intake.select :close @10:31:11.24 SSV.dial :exhaust @10:31:12.05 Exhaust.select :open @10:31:12.15 SS.reconfigure SSexhale @10:31:12.19 SS.seek 0.00ml @10:31:20.95 Exhaust.select :close @10:31:21.05 SSV.dial CBV @10:31:21.66 CBV.dial SSV @10:31:22.48 Allowing 185s for 25.0ml of remaining 900.0ml @10:31:22.49 Intake.select :open @10:31:24.62 Intake @46.7psia, vacuum from 20.0 to 24.0psi @10:31:24.64 SS.reconfigure forSampling @10:31:24.67 SS.seek 25.00ml @10:31:36.18 delay 5 seconds @10:31:37.26 Vacuum too high -- waiting up to 40s @10:31:37.53 Intake @44.9psia, vacuum from 20.0 to 24.0psi @10:31:37.55 SS.reconfigure forSampling @10:31:37.58 SS.seek 25.00ml @10:31:41.24 AS.reconfigure ASslow2 @10:31:41.27 AS.seek 0.454ml @10:32:17.39 delay 1:00 @10:32:18.34 Stroke completed -- waiting for pressure to equalize ... @10:32:19.57 Intake.select :close @10:32:19.71 SSV.dial :exhaust @10:32:20.50 Exhaust.select :open @10:32:20.60 SS.reconfigure SSexhale @10:32:20.64 SS.seek 0.00ml @10:32:29.74 Exhaust.select :close @10:32:29.84 SSV.dial CBV @10:32:29.86 CBV.dial SSV @10:32:30.66 Allowing 185s for 25.0ml of remaining 875.0ml @10:32:30.68 Intake.select :open @10:32:32.81 Intake @45.9psia, vacuum from 20.0 to 24.0psi @10:32:32.83 SS.reconfigure forSampling @10:32:32.86 SS.seek 25.00ml @10:32:48.75 Vacuum too high -- waiting up to 40s @10:32:49.02 Intake @45.7psia, vacuum from 20.0 to 24.0psi @10:32:49.03 SS.reconfigure forSampling @10:32:49.07 SS.seek 25.00ml @10:33:17.41 pushing eluate to holding coil @10:33:17.47 AS.seek 0.294ml @10:34:10.16 Stroke completed -- waiting for pressure to equalize ... @10:34:11.96 Intake.select :close @10:34:12.09 SSV.dial :exhaust @10:34:12.89 Exhaust.select :open @10:34:12.99 SS.reconfigure SSexhale @10:34:13.03 SS.seek 0.00ml @10:34:22.11 Exhaust.select :close @10:34:22.21 SSV.dial CBV @10:34:22.23 CBV.dial SSV @10:34:23.03 Allowing 185s for 25.0ml of remaining 850.0ml @10:34:23.04 Intake.select :open @10:34:25.18 Intake @45.5psia, vacuum from 20.0 to 24.0psi @10:34:25.20 SS.reconfigure forSampling @10:34:25.23 SS.seek 25.00ml @10:36:00.65 delay 30 seconds @10:36:09.40 Vacuum too high -- waiting up to 40s @10:36:10.10 Intake @45.3psia, vacuum from 20.0 to 24.0psi @10:36:10.11 SS.reconfigure forSampling @10:36:10.15 SS.seek 25.00ml @10:36:17.14 Stroke completed -- waiting for pressure to equalize ... @10:36:19.53 Intake.select :close @10:36:19.66 SSV.dial :exhaust @10:36:20.46 Exhaust.select :open @10:36:20.56 SS.reconfigure SSexhale @10:36:20.60 SS.seek 0.00ml @10:36:29.21 Exhaust.select :close @10:36:29.31 SSV.dial CBV @10:36:29.33 CBV.dial SSV @10:36:30.15 Allowing 185s for 25.0ml of remaining 825.0ml @10:36:30.16 Intake.select :open @10:36:30.66 ATV.dial :waste,via => air3 @10:36:32.19 AS.reconfigure ASconfig @10:36:32.22 AS.seek empty @10:36:32.30 Intake @45.8psia, vacuum from 20.0 to 24.0psi @10:36:32.31 SS.reconfigure forSampling @10:36:32.34 SS.seek 25.00ml @10:36:40.02 Vacuum too high -- waiting up to 40s @10:36:40.51 Intake @46.0psia, vacuum from 20.0 to 24.0psi @10:36:40.52 SS.reconfigure forSampling @10:36:40.56 SS.seek 25.00ml @10:36:44.93 Vacuum too high -- waiting up to 40s @10:36:45.41 Intake @46.1psia, vacuum from 20.0 to 24.0psi @10:36:45.42 SS.reconfigure forSampling @10:36:45.46 SS.seek 25.00ml @10:36:49.73 Parking Bleach in LysateSource line and SPEcolumn @10:36:49.74 ATV.dial :air3 @10:36:50.33 AS.reconfigure ASslow1 @10:36:50.36 AS.seek 0.029ml @10:36:50.75 Vacuum too high -- waiting up to 40s @10:36:51.23 Intake @46.2psia, vacuum from 20.0 to 24.0psi @10:36:51.25 SS.reconfigure forSampling @10:36:51.28 SS.seek 25.00ml @10:36:54.65 Vacuum too high -- waiting up to 40s @10:36:55.76 Intake @46.0psia, vacuum from 20.0 to 24.0psi @10:36:55.78 SS.reconfigure forSampling @10:36:55.81 SS.seek 25.00ml @10:36:56.78 ATV.dial :ElutionWater @10:36:57.26 AS.seek 0.059ml @10:36:59.69 Vacuum too high -- waiting up to 40s @10:37:00.19 Intake @45.5psia, vacuum from 20.0 to 24.0psi @10:37:00.21 SS.reconfigure forSampling @10:37:00.24 SS.seek 25.00ml @10:37:03.33 Vacuum too high -- waiting up to 40s @10:37:03.64 ATV.dial :air3 @10:37:03.83 SS.seek 25.00ml @10:37:04.03 AS.seek 0.069ml @10:37:06.34 delay 2 seconds @10:37:08.35 ATV.dial :bleach @10:37:08.73 AS.seek 0.709ml @10:37:09.34 Vacuum too high -- waiting up to 40s @10:37:10.05 Intake @45.7psia, vacuum from 20.0 to 24.0psi @10:37:10.07 SS.reconfigure forSampling @10:37:10.10 SS.seek 25.00ml @10:37:13.71 Vacuum too high -- waiting up to 40s @10:37:14.42 Intake @45.3psia, vacuum from 20.0 to 24.0psi @10:37:14.44 SS.reconfigure forSampling @10:37:14.47 SS.seek 25.00ml @10:37:17.86 Vacuum too high -- waiting up to 40s @10:37:18.56 Intake @45.3psia, vacuum from 20.0 to 24.0psi @10:37:18.58 SS.reconfigure forSampling @10:37:18.61 SS.seek 25.00ml @10:37:21.86 Vacuum too high -- waiting up to 40s @10:37:22.56 Intake @44.5psia, vacuum from 20.0 to 24.0psi @10:37:22.57 SS.reconfigure forSampling @10:37:22.61 SS.seek 25.00ml @10:37:26.93 Vacuum too high -- waiting up to 40s @10:37:27.62 Intake @44.3psia, vacuum from 20.0 to 24.0psi @10:37:27.63 SS.reconfigure forSampling @10:37:27.67 SS.seek 25.00ml @10:37:31.39 Vacuum too high -- waiting up to 40s @10:37:32.31 Intake @45.7psia, vacuum from 20.0 to 24.0psi @10:37:32.32 SS.reconfigure forSampling @10:37:32.36 SS.seek 25.00ml @10:37:35.25 Vacuum too high -- waiting up to 40s @10:37:35.95 Intake @45.0psia, vacuum from 20.0 to 24.0psi @10:37:35.96 SS.reconfigure forSampling @10:37:36.00 SS.seek 25.00ml @10:37:39.00 Vacuum too high -- waiting up to 40s @10:37:39.69 Intake @44.5psia, vacuum from 20.0 to 24.0psi @10:37:39.70 SS.reconfigure forSampling @10:37:39.74 SS.seek 25.00ml @10:37:42.86 Vacuum too high -- waiting up to 40s @10:37:43.77 Intake @46.2psia, vacuum from 20.0 to 24.0psi @10:37:43.78 SS.reconfigure forSampling @10:37:43.82 SS.seek 25.00ml @10:37:46.50 Vacuum too high -- waiting up to 40s @10:37:47.20 Intake @45.2psia, vacuum from 20.0 to 24.0psi @10:37:47.22 SS.reconfigure forSampling @10:37:47.25 SS.seek 25.00ml @10:37:50.00 Vacuum too high -- waiting up to 40s @10:37:50.70 Intake @45.5psia, vacuum from 20.0 to 24.0psi @10:37:50.71 SS.reconfigure forSampling @10:37:50.74 SS.seek 25.00ml @10:37:53.46 Vacuum too high -- waiting up to 40s @10:37:54.82 Intake @46.6psia, vacuum from 20.0 to 24.0psi @10:37:54.84 SS.reconfigure forSampling @10:37:54.87 SS.seek 25.00ml @10:37:57.87 Vacuum too high -- waiting up to 40s @10:37:58.57 Intake @46.5psia, vacuum from 20.0 to 24.0psi @10:37:58.59 SS.reconfigure forSampling @10:37:58.62 SS.seek 25.00ml @10:38:00.71 Vacuum too high -- waiting up to 40s @10:38:01.43 Intake @46.1psia, vacuum from 20.0 to 24.0psi @10:38:01.45 SS.reconfigure forSampling @10:38:01.48 SS.seek 25.00ml @10:38:04.00 Vacuum too high -- waiting up to 40s @10:38:04.71 Intake @45.9psia, vacuum from 20.0 to 24.0psi @10:38:04.73 SS.reconfigure forSampling @10:38:04.76 SS.seek 25.00ml @10:38:07.09 Vacuum too high -- waiting up to 40s @10:38:07.80 Intake @45.8psia, vacuum from 20.0 to 24.0psi @10:38:07.82 SS.reconfigure forSampling @10:38:07.85 SS.seek 25.00ml @10:38:10.43 Vacuum too high -- waiting up to 40s @10:38:11.13 Intake @46.8psia, vacuum from 20.0 to 24.0psi @10:38:11.15 SS.reconfigure forSampling @10:38:11.18 SS.seek 25.00ml @10:38:13.28 Vacuum too high -- waiting up to 40s @10:38:13.99 Intake @46.0psia, vacuum from 20.0 to 24.0psi @10:38:14.01 SS.reconfigure forSampling @10:38:14.04 SS.seek 25.00ml @10:38:16.28 Vacuum too high -- waiting up to 40s @10:38:17.00 Intake @45.8psia, vacuum from 20.0 to 24.0psi @10:38:17.01 SS.reconfigure forSampling @10:38:17.04 SS.seek 25.00ml @10:38:19.37 Vacuum too high -- waiting up to 40s @10:38:20.08 Intake @45.6psia, vacuum from 20.0 to 24.0psi @10:38:20.09 SS.reconfigure forSampling @10:38:20.13 SS.seek 25.00ml @10:38:22.52 Vacuum too high -- waiting up to 40s @10:38:23.24 SS.seek 25.00ml @10:38:25.59 Vacuum too high -- waiting up to 40s @10:38:26.50 Intake @47.0psia, vacuum from 20.0 to 24.0psi @10:38:26.51 SS.reconfigure forSampling @10:38:26.55 SS.seek 25.00ml @10:38:28.59 Vacuum too high -- waiting up to 40s @10:38:29.30 Intake @45.7psia, vacuum from 20.0 to 24.0psi @10:38:29.32 SS.reconfigure forSampling @10:38:29.35 SS.seek 25.00ml @10:38:31.53 Vacuum too high -- waiting up to 40s @10:38:32.29 Intake @45.2psia, vacuum from 20.0 to 24.0psi @10:38:32.30 SS.reconfigure forSampling @10:38:32.34 SS.seek 25.00ml @10:38:34.59 Vacuum too high -- waiting up to 40s @10:38:35.28 Intake @45.1psia, vacuum from 20.0 to 24.0psi @10:38:35.30 SS.reconfigure forSampling @10:38:35.33 SS.seek 25.00ml @10:38:37.37 Vacuum too high -- waiting up to 40s @10:38:38.08 SS.seek 25.00ml @10:38:40.24 Vacuum too high -- waiting up to 40s @10:38:41.65 Intake @46.2psia, vacuum from 20.0 to 24.0psi @10:38:41.67 SS.reconfigure forSampling @10:38:41.70 SS.seek 25.00ml @10:38:44.02 Vacuum too high -- waiting up to 40s @10:38:44.73 Intake @45.2psia, vacuum from 20.0 to 24.0psi @10:38:44.75 SS.reconfigure forSampling @10:38:44.78 SS.seek 25.00ml @10:38:46.84 Vacuum too high -- waiting up to 40s @10:38:47.55 Intake @45.2psia, vacuum from 20.0 to 24.0psi @10:38:47.57 SS.reconfigure forSampling @10:38:47.60 SS.seek 25.00ml @10:38:49.62 Vacuum too high -- waiting up to 40s @10:38:50.33 Intake @45.6psia, vacuum from 20.0 to 24.0psi @10:38:50.35 SS.reconfigure forSampling @10:38:50.38 SS.seek 25.00ml @10:38:52.37 Vacuum too high -- waiting up to 40s @10:38:53.09 Intake @45.2psia, vacuum from 20.0 to 24.0psi @10:38:53.10 SS.reconfigure forSampling @10:38:53.14 SS.seek 25.00ml @10:38:55.21 Vacuum too high -- waiting up to 40s @10:38:55.92 Intake @45.3psia, vacuum from 20.0 to 24.0psi @10:38:55.93 SS.reconfigure forSampling @10:38:55.97 SS.seek 25.00ml @10:38:58.12 Vacuum too high -- waiting up to 40s @10:38:58.83 Intake @45.2psia, vacuum from 20.0 to 24.0psi @10:38:58.85 SS.reconfigure forSampling @10:38:58.88 SS.seek 25.00ml @10:39:00.77 Vacuum too high -- waiting up to 40s @10:39:01.48 Intake @45.6psia, vacuum from 20.0 to 24.0psi @10:39:01.49 SS.reconfigure forSampling @10:39:01.53 SS.seek 25.00ml @10:39:03.14 Stroke completed -- waiting for pressure to equalize ... @10:39:06.72 Intake.select :close @10:39:06.85 SSV.dial :exhaust @10:39:07.66 Exhaust.select :open @10:39:07.76 SS.reconfigure SSexhale @10:39:07.80 SS.seek 0.00ml @10:39:16.30 Exhaust.select :close @10:39:16.40 SSV.dial CBV @10:39:16.42 CBV.dial SSV @10:39:17.24 Allowing 185s for 25.0ml of remaining 800.0ml @10:39:17.25 Intake.select :open @10:39:19.39 Intake @46.0psia, vacuum from 20.0 to 24.0psi @10:39:19.41 SS.reconfigure forSampling @10:39:19.44 SS.seek 25.00ml @10:39:19.54 delay 3 seconds @10:39:21.87 Vacuum too high -- waiting up to 40s @10:39:22.36 Intake @45.8psia, vacuum from 20.0 to 24.0psi @10:39:22.37 SS.reconfigure forSampling @10:39:22.41 SS.seek 25.00ml @10:39:22.55 ATV.dial :air3 @10:39:22.91 delay 3 seconds @10:39:23.77 Vacuum too high -- waiting up to 40s @10:39:24.48 Intake @45.8psia, vacuum from 20.0 to 24.0psi @10:39:24.50 SS.reconfigure forSampling @10:39:24.53 SS.seek 25.00ml @10:39:25.96 AS.seek 0.734ml @10:39:26.24 Vacuum too high -- waiting up to 40s @10:39:26.95 Intake @45.5psia, vacuum from 20.0 to 24.0psi @10:39:26.97 SS.reconfigure forSampling @10:39:27.00 SS.seek 25.00ml @10:39:28.90 Vacuum too high -- waiting up to 40s @10:39:29.59 Intake @45.8psia, vacuum from 20.0 to 24.0psi @10:39:29.61 SS.reconfigure forSampling @10:39:29.64 SS.seek 25.00ml @10:39:30.96 Vacuum too high -- waiting up to 40s @10:39:31.69 delay 3 seconds @10:39:31.93 Intake @46.2psia, vacuum from 20.0 to 24.0psi @10:39:31.95 SS.reconfigure forSampling @10:39:31.98 SS.seek 25.00ml @10:39:33.83 Vacuum too high -- waiting up to 40s @10:39:34.56 Intake @46.2psia, vacuum from 20.0 to 24.0psi @10:39:34.57 SS.reconfigure forSampling @10:39:34.60 SS.seek 25.00ml @10:39:34.69 ATV.dial :bleach @10:39:35.10 AS.seek 0.719ml @10:39:36.30 Vacuum too high -- waiting up to 40s @10:39:37.01 Intake @46.0psia, vacuum from 20.0 to 24.0psi @10:39:37.03 SS.reconfigure forSampling @10:39:37.06 SS.seek 25.00ml @10:39:38.41 delay 3 seconds @10:39:38.55 Vacuum too high -- waiting up to 40s @10:39:39.31 Intake @47.0psia, vacuum from 20.0 to 24.0psi @10:39:39.33 SS.reconfigure forSampling @10:39:39.36 SS.seek 25.00ml @10:39:40.68 Vacuum too high -- waiting up to 40s @10:39:41.17 Intake @45.9psia, vacuum from 20.0 to 24.0psi @10:39:41.19 SS.reconfigure forSampling @10:39:41.22 SS.seek 25.00ml @10:39:41.42 ATV.dial :AIV @10:39:42.49 Vacuum too high -- waiting up to 40s @10:39:42.56 AS.reconfigure ASconfig @10:39:42.59 AS.seek 0.015ml @10:39:43.20 Intake @45.4psia, vacuum from 20.0 to 24.0psi @10:39:43.22 SS.reconfigure forSampling @10:39:43.25 SS.seek 25.00ml @10:39:45.12 Vacuum too high -- waiting up to 40s @10:39:45.83 Intake @45.2psia, vacuum from 20.0 to 24.0psi @10:39:45.84 SS.reconfigure forSampling @10:39:45.88 SS.seek 25.00ml @10:39:47.74 Vacuum too high -- waiting up to 40s @10:39:48.65 Intake @45.3psia, vacuum from 20.0 to 24.0psi @10:39:48.67 SS.reconfigure forSampling @10:39:48.70 SS.seek 25.00ml @10:39:50.68 Vacuum too high -- waiting up to 40s @10:39:51.60 Intake @46.7psia, vacuum from 20.0 to 24.0psi @10:39:51.62 SS.reconfigure forSampling @10:39:51.65 SS.seek 25.00ml @10:39:53.05 Vacuum too high -- waiting up to 40s @10:39:53.53 Intake @45.7psia, vacuum from 20.0 to 24.0psi @10:39:53.55 SS.reconfigure forSampling @10:39:53.58 SS.seek 25.00ml @10:39:55.18 Vacuum too high -- waiting up to 40s @10:39:56.08 Intake @45.2psia, vacuum from 20.0 to 24.0psi @10:39:56.09 SS.reconfigure forSampling @10:39:56.13 SS.seek 25.00ml @10:39:58.49 Vacuum too high -- waiting up to 40s @10:39:59.40 Intake @45.3psia, vacuum from 20.0 to 24.0psi @10:39:59.41 SS.reconfigure forSampling @10:39:59.45 SS.seek 25.00ml @10:40:01.40 Vacuum too high -- waiting up to 40s @10:40:02.31 Intake @45.7psia, vacuum from 20.0 to 24.0psi @10:40:02.33 SS.reconfigure forSampling @10:40:02.36 SS.seek 25.00ml @10:40:04.40 Vacuum too high -- waiting up to 40s @10:40:05.32 Intake @45.8psia, vacuum from 20.0 to 24.0psi @10:40:06.04 SS.reconfigure forSampling @10:40:06.07 SS.seek 25.00ml @10:40:08.36 Vacuum too high -- waiting up to 40s @10:40:09.29 Intake @46.2psia, vacuum from 20.0 to 24.0psi @10:40:09.31 SS.reconfigure forSampling @10:40:09.34 SS.seek 25.00ml @10:40:10.93 Vacuum too high -- waiting up to 40s @10:40:11.64 Intake @46.3psia, vacuum from 20.0 to 24.0psi @10:40:11.66 SS.reconfigure forSampling @10:40:11.69 SS.seek 25.00ml @10:40:13.08 Vacuum too high -- waiting up to 40s @10:40:13.79 Intake @45.8psia, vacuum from 20.0 to 24.0psi @10:40:13.81 SS.reconfigure forSampling @10:40:13.84 SS.seek 25.00ml @10:40:15.52 Vacuum too high -- waiting up to 40s @10:40:16.45 Intake @45.5psia, vacuum from 20.0 to 24.0psi @10:40:16.47 SS.reconfigure forSampling @10:40:16.50 SS.seek 25.00ml @10:40:18.55 Vacuum too high -- waiting up to 40s @10:40:19.47 Intake @46.1psia, vacuum from 20.0 to 24.0psi @10:40:19.48 SS.reconfigure forSampling @10:40:19.52 SS.seek 25.00ml @10:40:21.30 Vacuum too high -- waiting up to 40s @10:40:22.23 Intake @46.0psia, vacuum from 20.0 to 24.0psi @10:40:22.25 SS.reconfigure forSampling @10:40:22.28 SS.seek 25.00ml @10:40:23.93 ATV.dial :waste @10:40:24.18 Vacuum too high -- waiting up to 40s @10:40:24.90 Intake @45.8psia, vacuum from 20.0 to 24.0psi @10:40:24.91 SS.reconfigure forSampling @10:40:24.94 SS.seek 25.00ml @10:40:25.25 AS.seek empty @10:40:26.44 ATV.dial :air3 @10:40:26.58 Vacuum too high -- waiting up to 40s @10:40:27.09 AS.reconfigure ASslow1 @10:40:27.13 AS.seek 0.009ml @10:40:27.52 Intake @45.8psia, vacuum from 20.0 to 24.0psi @10:40:27.54 SS.reconfigure forSampling @10:40:27.57 SS.seek 25.00ml @10:40:29.39 Vacuum too high -- waiting up to 40s @10:40:29.46 delay 2 seconds @10:40:30.31 Intake @46.1psia, vacuum from 20.0 to 24.0psi @10:40:30.33 SS.reconfigure forSampling @10:40:30.36 SS.seek 25.00ml @10:40:31.47 ATV.dial :bleach @10:40:31.87 AS.seek 0.579ml @10:40:32.08 Vacuum too high -- waiting up to 40s @10:40:33.01 Intake @45.9psia, vacuum from 20.0 to 24.0psi @10:40:33.02 SS.reconfigure forSampling @10:40:33.06 SS.seek 25.00ml @10:40:34.89 Vacuum too high -- waiting up to 40s @10:40:35.81 Intake @45.8psia, vacuum from 20.0 to 24.0psi @10:40:35.83 SS.reconfigure forSampling @10:40:35.86 SS.seek 25.00ml @10:40:37.86 Vacuum too high -- waiting up to 40s @10:40:38.77 Intake @46.6psia, vacuum from 20.0 to 24.0psi @10:40:38.78 SS.reconfigure forSampling @10:40:38.82 SS.seek 25.00ml @10:40:41.39 Vacuum too high -- waiting up to 40s @10:40:42.11 Intake @45.9psia, vacuum from 20.0 to 24.0psi @10:40:42.12 SS.reconfigure forSampling @10:40:42.15 SS.seek 25.00ml @10:40:43.96 Vacuum too high -- waiting up to 40s @10:40:44.89 Intake @46.4psia, vacuum from 20.0 to 24.0psi @10:40:44.91 SS.reconfigure forSampling @10:40:44.94 SS.seek 25.00ml @10:40:46.55 Vacuum too high -- waiting up to 40s @10:40:47.34 Intake @45.9psia, vacuum from 20.0 to 24.0psi @10:40:47.35 SS.reconfigure forSampling @10:40:47.43 SS.seek 25.00ml @10:40:49.14 Vacuum too high -- waiting up to 40s @10:40:50.07 Intake @46.7psia, vacuum from 20.0 to 24.0psi @10:40:50.08 SS.reconfigure forSampling @10:40:50.12 SS.seek 25.00ml @10:40:51.71 Vacuum too high -- waiting up to 40s @10:40:52.42 Intake @46.9psia, vacuum from 20.0 to 24.0psi @10:40:52.44 SS.reconfigure forSampling @10:40:52.47 SS.seek 25.00ml @10:40:53.67 Vacuum too high -- waiting up to 40s @10:40:54.37 Intake @46.2psia, vacuum from 20.0 to 24.0psi @10:40:54.39 SS.reconfigure forSampling @10:40:54.42 SS.seek 25.00ml @10:40:55.96 Vacuum too high -- waiting up to 40s @10:40:56.87 Intake @45.6psia, vacuum from 20.0 to 24.0psi @10:40:56.89 SS.reconfigure forSampling @10:40:56.92 SS.seek 25.00ml @10:40:58.58 Vacuum too high -- waiting up to 40s @10:40:59.50 Intake @45.4psia, vacuum from 20.0 to 24.0psi @10:40:59.52 SS.reconfigure forSampling @10:40:59.55 SS.seek 25.00ml @10:41:01.52 Vacuum too high -- waiting up to 40s @10:41:02.66 Intake @45.9psia, vacuum from 20.0 to 24.0psi @10:41:02.68 SS.reconfigure forSampling @10:41:02.71 SS.seek 25.00ml @10:41:04.86 Vacuum too high -- waiting up to 40s @10:41:05.78 Intake @46.2psia, vacuum from 20.0 to 24.0psi @10:41:05.80 SS.reconfigure forSampling @10:41:05.83 SS.seek 25.00ml @10:41:07.61 Vacuum too high -- waiting up to 40s @10:41:08.53 Intake @45.3psia, vacuum from 20.0 to 24.0psi @10:41:08.54 SS.reconfigure forSampling @10:41:08.58 SS.seek 25.00ml @10:41:10.45 Vacuum too high -- waiting up to 40s @10:41:11.36 Intake @45.8psia, vacuum from 20.0 to 24.0psi @10:41:11.38 SS.reconfigure forSampling @10:41:11.41 SS.seek 25.00ml @10:41:13.30 Vacuum too high -- waiting up to 40s @10:41:14.22 Intake @45.8psia, vacuum from 20.0 to 24.0psi @10:41:14.24 SS.reconfigure forSampling @10:41:14.27 SS.seek 25.00ml @10:41:16.05 Vacuum too high -- waiting up to 40s @10:41:17.05 Intake @45.6psia, vacuum from 20.0 to 24.0psi @10:41:17.07 SS.reconfigure forSampling @10:41:17.10 SS.seek 25.00ml @10:41:18.92 Vacuum too high -- waiting up to 40s @10:41:19.85 Intake @46.8psia, vacuum from 20.0 to 24.0psi @10:41:19.87 SS.reconfigure forSampling @10:41:19.90 SS.seek 25.00ml @10:41:21.58 Vacuum too high -- waiting up to 40s @10:41:22.53 Intake @46.0psia, vacuum from 20.0 to 24.0psi @10:41:22.55 SS.reconfigure forSampling @10:41:22.58 SS.seek 25.00ml @10:41:24.14 Vacuum too high -- waiting up to 40s @10:41:25.08 SS.seek 25.00ml @10:41:26.70 Vacuum too high -- waiting up to 40s @10:41:27.63 Intake @45.6psia, vacuum from 20.0 to 24.0psi @10:41:27.65 SS.reconfigure forSampling @10:41:27.68 SS.seek 25.00ml @10:41:29.39 Vacuum too high -- waiting up to 40s @10:41:30.32 Intake @45.4psia, vacuum from 20.0 to 24.0psi @10:41:30.33 SS.reconfigure forSampling @10:41:30.37 SS.seek 25.00ml @10:41:32.11 Vacuum too high -- waiting up to 40s @10:41:33.03 Intake @45.0psia, vacuum from 20.0 to 24.0psi @10:41:33.04 SS.reconfigure forSampling @10:41:33.07 SS.seek 25.00ml @10:41:34.86 Vacuum too high -- waiting up to 40s @10:41:35.99 Intake @46.9psia, vacuum from 20.0 to 24.0psi @10:41:36.01 SS.reconfigure forSampling @10:41:36.04 SS.seek 25.00ml @10:41:37.70 Vacuum too high -- waiting up to 40s @10:41:38.39 Intake @45.3psia, vacuum from 20.0 to 24.0psi @10:41:38.41 SS.reconfigure forSampling @10:41:38.44 SS.seek 25.00ml @10:41:39.83 Vacuum too high -- waiting up to 40s @10:41:40.75 Intake @45.5psia, vacuum from 20.0 to 24.0psi @10:41:40.77 SS.reconfigure forSampling @10:41:40.80 SS.seek 25.00ml @10:41:42.45 Vacuum too high -- waiting up to 40s @10:41:43.37 Intake @44.9psia, vacuum from 20.0 to 24.0psi @10:41:43.38 SS.reconfigure forSampling @10:41:43.42 SS.seek 25.00ml @10:41:45.17 Vacuum too high -- waiting up to 40s @10:41:46.08 Intake @44.9psia, vacuum from 20.0 to 24.0psi @10:41:46.10 SS.reconfigure forSampling @10:41:46.13 SS.seek 25.00ml @10:41:47.76 Vacuum too high -- waiting up to 40s @10:41:48.69 Intake @45.2psia, vacuum from 20.0 to 24.0psi @10:41:48.71 SS.reconfigure forSampling @10:41:48.74 SS.seek 25.00ml @10:41:50.55 Vacuum too high -- waiting up to 40s @10:41:52.19 Intake @46.1psia, vacuum from 20.0 to 24.0psi @10:41:52.21 SS.reconfigure forSampling @10:41:52.24 SS.seek 25.00ml @10:41:54.08 Vacuum too high -- waiting up to 40s @10:41:54.79 Intake @45.2psia, vacuum from 20.0 to 24.0psi @10:41:54.81 SS.reconfigure forSampling @10:41:54.84 SS.seek 25.00ml @10:41:56.36 Vacuum too high -- waiting up to 40s @10:41:57.29 Intake @44.9psia, vacuum from 20.0 to 24.0psi @10:41:57.31 SS.reconfigure forSampling @10:41:57.34 SS.seek 25.00ml @10:41:59.08 Vacuum too high -- waiting up to 40s @10:42:00.00 Intake @45.2psia, vacuum from 20.0 to 24.0psi @10:42:00.02 SS.reconfigure forSampling @10:42:00.05 SS.seek 25.00ml @10:42:01.61 Vacuum too high -- waiting up to 40s @10:42:02.52 Intake @45.0psia, vacuum from 20.0 to 24.0psi @10:42:02.54 SS.reconfigure forSampling @10:42:02.57 SS.seek 25.00ml @10:42:04.23 Vacuum too high -- waiting up to 40s @10:42:05.15 Intake @44.9psia, vacuum from 20.0 to 24.0psi @10:42:05.17 SS.reconfigure forSampling @10:42:05.20 SS.seek 25.00ml @10:42:06.80 Vacuum too high -- waiting up to 40s @10:42:07.95 Intake @46.1psia, vacuum from 20.0 to 24.0psi @10:42:07.97 SS.reconfigure forSampling @10:42:08.00 SS.seek 25.00ml @10:42:09.64 Vacuum too high -- waiting up to 40s @10:42:10.56 Intake @45.4psia, vacuum from 20.0 to 24.0psi @10:42:10.58 SS.reconfigure forSampling @10:42:10.61 SS.seek 25.00ml @10:42:12.17 Vacuum too high -- waiting up to 40s @10:42:13.11 Intake @44.7psia, vacuum from 20.0 to 24.0psi @10:42:13.13 SS.reconfigure forSampling @10:42:13.16 SS.seek 25.00ml @10:42:14.70 Vacuum too high -- waiting up to 40s @10:42:15.84 Intake @45.4psia, vacuum from 20.0 to 24.0psi @10:42:15.86 SS.reconfigure forSampling @10:42:15.89 SS.seek 25.00ml @10:42:17.51 Vacuum too high -- waiting up to 40s @10:42:18.43 Intake @45.0psia, vacuum from 20.0 to 24.0psi @10:42:18.45 SS.reconfigure forSampling @10:42:18.48 SS.seek 25.00ml @10:42:20.11 Vacuum too high -- waiting up to 40s @10:42:21.03 Intake @44.6psia, vacuum from 20.0 to 24.0psi @10:42:21.05 SS.reconfigure forSampling @10:42:21.08 SS.seek 25.00ml @10:42:22.26 SS.reconfigure SSconfig @10:42:22.29 SS.hold @10:42:22.44 Intake.select :close @10:42:22.59 ShallowSampler::Clogged in WCR -- progress too slow after 223.0ml @10:42:22.60 SSV.dial :exhaust @10:42:23.40 Exhaust.select :open @10:42:23.51 SS.reconfigure SSexhale @10:42:23.55 SS.seek 0.00ml @10:42:28.39 delay 3 seconds @10:42:31.69 Exhaust.select :close @10:42:31.80 Sampled 223.0ml @10:42:31.81 collectSample: Complete @10:42:31.82 Drying puck before fixing for 04:00.00 @10:42:31.83 CBV.dial :waste2b @10:42:31.88 ATV.dial :air3 @10:42:32.32 delay 3 seconds @10:42:32.62 delay 1:00 @10:42:34.79 Sleepy.power :sampler,:OFF @10:42:35.37 AS.seek 0.604ml @10:42:40.73 delay 3 seconds @10:42:43.74 ATV.dial :bleach @10:42:44.11 AS.seek 0.589ml @10:42:47.37 delay 3 seconds @10:42:50.38 ATV.dial :ASV @10:42:51.36 ASV.dial :wasteR @10:42:52.52 AS.seek 0.000ml @10:43:32.62 CSV.dial :air @10:43:33.07 CS.reconfigure CSfast @10:43:33.10 CS.seek 10.000ml @10:43:56.94 CBV.dial between CSV and waste2b @10:43:57.26 Pumping wcr puck from tube 2 up to 55psi... @10:43:57.30 CBV.dial SSV @10:43:58.74 CBV.dial between CSV and waste2b @10:43:59.69 CSV.dial between air and CTV @10:44:00.09 CS.seek 7.108ml @10:44:07.82 CTV.dial CSV,avoiding => air @10:44:07.84 CSV.dial CTV @10:44:08.60 CS.reconfigure CSpressurize @10:44:08.63 CS.seek 0.000ml @10:44:24.11 CBV.dial :waste2b @10:44:24.43 CS.reconfigure CSconfig @10:44:24.46 CS.seek empty @10:44:28.19 delay 1:00 @10:44:52.93 delay 1:00 @10:45:28.20 CTV.dial :air,avoiding => intake @10:45:29.46 Fix #1 of 2 (with rnal) for 02:00.00 @10:45:29.47 CS Pulling 2ml of rnal CSV.dial CSR @10:45:29.90 CSR.select :rnal @10:45:30.00 CS.seek 2.000ml @10:45:35.90 delay 5 seconds @10:45:40.91 CSR.select :air @10:45:41.00 CS.reconfigure CSfast @10:45:41.04 CS.seek 10.000ml @10:45:52.94 AFV.select :carrierSolution @10:45:53.03 delay 2 seconds @10:45:55.04 AS.reconfigure ASconfig @10:45:55.07 AS.seek 1.000ml @10:46:00.12 CBV.dial between CSV and waste2b @10:46:00.46 Pumping wcr puck from tube 2 up to 55psi... @10:46:00.51 CBV.dial SSV @10:46:01.92 CBV.dial between CSV and waste2b @10:46:02.86 CSV.dial between air and CTV @10:46:03.52 CS.seek 7.019ml @10:46:11.13 CTV.dial CSV,avoiding => air @10:46:11.15 CSV.dial CTV @10:46:12.52 CS.reconfigure CSpressurize @10:46:12.55 CS.seek 0.000ml @10:46:30.33 delay 2:00 @10:46:53.66 delay 1 second @10:46:54.67 AFV.select :holdingCoil @10:46:54.76 delay 1 second @10:46:55.77 ATV.dial :ASV @10:46:55.78 ASV.dial :wasteR @10:46:55.79 AS.reconfigure ASslow1 @10:46:55.82 AS.seek 0.000ml @10:48:30.34 CBV.dial :waste2b @10:48:30.67 CS.reconfigure CSconfig @10:48:30.70 CS.seek empty @10:48:31.83 delay 1:00 @10:49:31.85 CTV.dial :air,avoiding => intake @10:49:33.11 Fix #2 of 2 (with rnal) for 02:00.00 @10:49:33.12 CS Pulling 2ml of rnal CSV.dial CSR @10:49:33.62 CSR.select :rnal @10:49:33.72 CS.seek 2.000ml @10:49:39.56 delay 5 seconds @10:49:44.57 CSR.select :air @10:49:44.66 CS.reconfigure CSfast @10:49:44.70 CS.seek 10.000ml @10:50:03.87 CBV.dial between CSV and waste2b @10:50:04.20 Pumping wcr puck from tube 2 up to 55psi... @10:50:04.24 CBV.dial SSV @10:50:05.66 CBV.dial between CSV and waste2b @10:50:06.59 CSV.dial between air and CTV @10:50:07.25 CS.seek 6.919ml @10:50:15.16 CTV.dial CSV,avoiding => air @10:50:15.18 CSV.dial CTV @10:50:16.53 CS.reconfigure CSpressurize @10:50:16.56 CS.seek 0.000ml @10:50:20.07 delay 20 seconds @10:50:34.23 delay 2:00 @10:50:40.08 ATV.dial :air3 @10:50:41.86 AS.seek 0.590ml @10:52:34.24 CBV.dial :waste2b @10:52:34.56 CS.reconfigure CSconfig @10:52:34.59 CS.seek empty @10:52:35.45 delay 1:00 @10:52:42.46 delay 3 seconds @10:52:45.47 ATV.dial :ASV @10:52:46.64 ASV.dial :wasteR @10:52:46.70 AS.seek 0.090ml @10:53:35.46 CTV.dial :air,avoiding => intake @10:53:36.77 Storing wcr puck in Collection Clamp to tube 1 @10:53:36.90 SC.reconfigure SCtight @10:53:36.94 SC.seek 1 @10:53:39.11 SC.reconfigure SCloose @10:53:39.29 Elbow.reconfigure TightElbow @10:53:39.32 Elbow.seek 1 @10:53:40.93 Forearm.reconfigure TightForearm @10:53:40.96 Forearm.seek 1 + 800 counts @10:53:42.05 SE.reconfigure SEpick @10:53:42.11 SE.seek beamBreak @10:53:43.20 SE.configure SEconfig @10:53:43.32 Elbow.reconfigure LimpElbow @10:53:43.36 Forearm.reconfigure LooseForearm @10:53:43.40 Forearm.seek 1 @10:53:43.62 Elbow.hold @10:53:43.67 Elbow.reconfigure TightElbow @10:53:43.75 SE.seek 19.05 Pucks below Top Plate @10:53:44.06 Forearm.coast @10:53:44.10 Forearm.seek 1 + 343 counts @10:53:44.41 Forearm.reconfigure TightForearm @10:53:44.45 Forearm.seek 1 + 800 counts @10:53:44.74 SE.seek 20.05 Pucks below Top Plate @10:53:45.28 Forearm.reconfigure LooseForearm @10:53:45.31 Forearm.seek retract @10:53:46.34 Elbow.seek CC @10:53:47.94 Elbow.reconfigure LooseElbow @10:53:48.02 Forearm.seek CC @10:53:48.96 CC.seek sealed @10:53:49.92 Hand.grip @10:53:50.69 CC.seek open @10:53:54.06 Forearm.seek retract @10:53:55.00 Elbow.reconfigure TightElbow @10:53:55.04 Elbow.seek 1 @10:53:56.68 Elbow.reconfigure LimpElbow @10:53:56.73 Forearm.seek 1 @10:53:57.68 Elbow.hold @10:53:57.74 Elbow.reconfigure TightElbow @10:53:57.77 Hand.release @10:53:58.65 Forearm.coast @10:53:58.69 Forearm.seek 1 + 331 counts @10:53:58.97 Forearm.reconfigure TightForearm @10:53:59.00 Forearm.seek 1 + 800 counts @10:53:59.22 SE.seek down @10:53:59.96 SE.coast @10:54:00.32 Forearm.reconfigure LooseForearm @10:54:00.36 Forearm.seek retract @10:54:01.28 SC.coast @10:54:01.31 Starting finalPurge @10:54:01.32 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @10:54:01.47 Elbow.seek FlushPuck::Garage @10:54:03.87 Elbow.reconfigure LooseElbow @10:54:03.95 Forearm.seek garage @10:54:04.97 Hand.grip @10:54:05.78 Forearm.seek retract @10:54:06.80 Elbow.reconfigure TightElbow @10:54:06.83 Elbow.seek CC @10:54:09.59 Elbow.reconfigure LooseElbow @10:54:09.67 Forearm.reconfigure TightForearm @10:54:09.70 Forearm.seek CC @10:54:10.83 CC.seek closed @10:54:14.59 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 2.04 @10:54:14.65 Hand.release @10:54:15.36 Forearm.reconfigure LooseForearm @10:54:15.39 Forearm.seek retract @10:54:16.31 Flushing the Collection Valve::Manifold @10:54:16.41 CS Pulling 5ml of flush CSV.dial CSR @10:54:16.92 CSR.select :flush @10:54:17.02 CS.seek 5.000ml @10:54:28.92 delay 30 seconds @10:54:30.57
delay 5 seconds @10:54:35.58 CSR.select :air @10:54:35.72 CS.reconfigure CSfast @10:54:35.75 CS.seek 7.500ml @10:54:42.32 CSV.dial :waste @10:54:43.99 CS.reconfigure CSconfig @10:54:44.02 CS.seek 0.000ml @10:54:58.93 ATV.dial :extSrc @10:54:59.44 delay 3 seconds @10:55:02.45 ATV.dial :ElutionWater @10:55:03.38 AS.seek 0.120ml @10:55:03.82
Flushing lower manifold's waste1 port @10:55:03.91 CSV.dial :flush @10:55:04.36 CS.reconfigure CSfast @10:55:04.39 CS.seek 5.000ml @10:55:09.81 ATV.dial :air3 @10:55:10.19 AS.seek 0.130ml @10:55:12.44 delay 2 seconds @10:55:14.45 ATV.dial :bleach @10:55:14.83 AS.seek 0.230ml @10:55:16.60
delay 5 seconds @10:55:21.61 CSV.dial :air @10:55:22.07 CS.seek 7.000ml @10:55:27.45 CSV.dial CTV @10:55:27.47
CTV.dial CSV @10:55:28.79 CBV.dial :waste1 @10:55:29.53 CS.seek 0.000ml @10:55:35.45 delay 3 seconds @10:55:38.46 ATV.dial :air3 @10:55:38.80 delay 3 seconds @10:55:41.86 AS.seek 0.255ml @10:55:46.26
Flushing lower manifold's waste2b port @10:55:46.35 CSV.dial :flush @10:55:47.13 delay 3 seconds @10:55:47.16
CS.seek 5.000ml @10:55:50.14 ATV.dial :bleach @10:55:50.53 AS.seek 0.240ml @10:55:53.83 delay 3 seconds @10:55:56.84 ATV.dial :ASV @10:55:57.72 ASV.dial :SPEdelivery @10:55:58.03 AFV.select :carrierSolution @10:55:58.13 delay 2 seconds @10:55:59.41
delay 5 seconds @10:56:00.14 AS.reconfigure ASconfig @10:56:00.18 AS.seek 0.500ml @10:56:04.42
CSV.dial :air @10:56:04.96 CS.seek 7.000ml @10:56:10.32 CSV.dial CTV @10:56:10.34
CTV.dial CSV @10:56:10.77 CBV.dial :waste2b @10:56:11.52 CS.seek 0.000ml @10:56:15.62 delay 1 second @10:56:16.63 AFV.select :holdingCoil @10:56:16.77 AS.reconfigure ASslow1 @10:56:16.80 AS.seek 0.170ml @10:56:28.04
Flushing upper manifold @10:56:28.13 CSV.dial :flush @10:56:28.92 CS.seek 5.000ml @10:56:41.69 delay 5 seconds @10:56:46.70 CSV.dial :air @10:56:47.26 CS.seek 7.001ml @10:56:52.72 CSV.dial CBV @10:56:52.74
CBV.dial CSV @10:56:53.86 CTV.dial :air @10:56:55.12 CS.seek 0.000ml @10:57:11.69 delay 7 seconds @10:57:18.70 CS.seek 3.000ml @10:57:24.37 SPE.hold 2:46:40,95.0C @10:57:24.39 delay 3:00 @10:57:26.41
CSV.dial :waste @10:57:26.93 CS.seek 0.000ml @10:57:34.87 CSV.dial :flush @10:57:35.32 CS.seek 3.000ml @10:57:43.28 delay 5 seconds @10:57:48.29 CSV.dial :air @10:57:48.77 CS.seek 5.000ml @10:57:54.44 CSV.dial :air @10:57:54.46 CS.seek 0.000ml @10:58:07.00 delay 7 seconds @10:58:14.01 CS.seek 3.000ml @10:58:21.94 CSV.dial :waste @10:58:22.76 CS.seek 0.000ml @10:58:30.66 Disinfecting external Intake with mfbkill @10:58:30.75 CSV.dial CSR @10:58:32.05 CSR.select :mfbkill @10:58:32.15 CS.seek 8.000ml @10:58:52.12 delay 5 seconds @10:58:57.13 CSR.select :air @10:58:57.22 CS.seek 10.000ml @10:59:02.78 CSV.dial CBV @10:59:02.80
CBV.dial CSV @10:59:04.30 CTV.dial :intake @10:59:05.01 Intake.select :open @10:59:05.11 CS.seek 2.000ml @10:59:25.03 Intake.select :close @10:59:25.12 CTV.dial :air @10:59:25.90 CS.reconfigure CSconfig @10:59:25.93 CS.seek 0.000ml @10:59:31.90 Flushing lower manifold's waste1 port @10:59:31.91 CS Pulling 4ml of mfbkill @10:59:31.92 CSV.dial CSR @10:59:33.00 CSR.select :mfbkill @10:59:33.10 CS.seek 4.000ml @10:59:44.00 delay 5 seconds @10:59:49.01 CSR.select :air @10:59:49.10 CS.reconfigure CSfast @10:59:49.14 CS.seek 10.000ml @11:00:04.25 CSV.dial CTV @11:00:04.27
CTV.dial CSV @11:00:05.60 CBV.dial :waste1 @11:00:06.07 CS.seek 0.000ml @11:00:24.40 AFV.select :carrierSolution @11:00:24.49 delay 2 seconds @11:00:26.50 AS.reconfigure ASconfig @11:00:26.53 AS.seek 1.000ml @11:00:30.21
Flushing lower manifold's waste2b port @11:00:30.22 CS Pulling 4ml of mfbkill @11:00:30.23 CSV.dial CSR @11:00:30.74 CSR.select :mfbkill @11:00:30.84 CS.reconfigure CSconfig @11:00:30.88 CS.seek 4.000ml @11:00:41.84 delay 5 seconds @11:00:46.85 CSR.select :air @11:00:46.94 CS.reconfigure CSfast @11:00:46.98 CS.seek 10.000ml @11:01:01.72 CSV.dial CTV @11:01:01.74
CTV.dial CSV @11:01:02.27 CBV.dial :waste2b @11:01:03.03 CS.seek 0.000ml @11:01:15.19 delay 1 second @11:01:16.20 AFV.select :holdingCoil @11:01:16.29 delay 1 second @11:01:17.30 ASV.dial :wasteD @11:01:18.31 AS.seek 0.000ml @11:01:26.68
Killing upper manifold @11:01:26.69 CS Pulling 4ml of mfbkill @11:01:26.70 CSV.dial CSR @11:01:27.21 CSR.select :mfbkill @11:01:27.31 CS.reconfigure CSconfig @11:01:27.35 CS.seek 4.000ml @11:01:38.27 delay 5 seconds @11:01:43.28 CSR.select :air @11:01:43.37 CS.reconfigure CSfast @11:01:43.41 CS.seek 10.000ml @11:01:58.02 CSV.dial CBV @11:01:58.04
CBV.dial CSV @11:01:59.07 CTV.dial :air @11:02:00.37 CS.seek 0.000ml @11:02:16.91 Priming Eluate @11:02:16.92 AFV.select :carrierSolution @11:02:17.01 delay 2 seconds @11:02:19.02 AS.seek 0.500ml @11:02:24.11
delay 7 seconds @11:02:31.12 CS.seek 3.000ml @11:02:38.83 CSV.dial :waste @11:02:39.28 CS.seek 0.000ml @11:02:46.96 Flushing upper manifold @11:02:47.05 CSV.dial :flush @11:02:48.07 CS.seek 5.000ml @11:02:48.45 delay 1 second @11:02:49.46 AFV.select :holdingCoil @11:02:49.55 ATV.dial ASV @11:02:49.56 ASV.dial :SPErecovery @11:02:50.22 Swish #1 of 2 @11:02:50.28 AS.reconfigure ASslow0 @11:02:50.31 AS.seek 0.600ml @11:03:00.39
delay 5 seconds @11:03:00.83 AS.seek 0.500ml @11:03:05.40
CSV.dial :air @11:03:05.90 CS.seek 7.000ml @11:03:11.34 Swish #2 of 2 @11:03:11.39 CSV.dial CBV @11:03:11.41
CBV.dial CSV @11:03:11.46 AS.seek 0.600ml @11:03:12.50
CTV.dial :air @11:03:12.51 CS.seek 0.000ml @11:03:21.95 AS.seek 0.500ml @11:03:29.24
delay 7 seconds @11:03:32.40 delay 3 seconds @11:03:35.46 AS.reconfigure ASslow1 @11:03:35.49 AS.seek 0.676ml @11:03:36.25
CS.seek 3.000ml @11:03:43.95 CSV.dial :waste @11:03:44.46 CS.seek 0.000ml @11:03:52.17 CSV.dial :flush @11:03:52.82 CS.seek 3.000ml @11:04:00.61 delay 5 seconds @11:04:05.62 CSV.dial :air @11:04:06.19 CS.seek 5.000ml @11:04:11.61 CSV.dial :air @11:04:11.63 CS.seek 0.000ml @11:04:11.67 delay 3 seconds @11:04:14.68 Eluate primed @11:04:14.69 ATV.dial :waste2 @11:04:15.30 AS.reconfigure ASnorm @11:04:15.33 AS.seek 0.000ml @11:04:23.86
delay 7 seconds @11:04:30.87 CS.seek 3.000ml @11:04:38.54 CSV.dial :waste @11:04:39.34 CS.seek 0.000ml @11:04:46.98 Flushing lower manifold's waste1 port @11:04:47.08 CSV.dial :flush @11:04:47.59 CS.seek 5.000ml @11:04:50.21 SPE.coast @11:04:50.29 ASV.dial :SPErecovery @11:04:50.30 Setting up PCR for HF183em -- writing /var/log/esp/HF183em14mar1011h224ml.pcr @11:04:50.31 Building PCR Slug from ASV with MM2 mix, PP4 primer @11:04:50.32 Priming with carrier @11:04:50.33 ATV.dial ARV @11:04:51.01 ARV.dial :carrier @11:04:51.29 AS.seek 0.500ml @11:04:59.86
delay 5 seconds @11:05:04.87 CSV.dial :air @11:05:05.34 CS.seek 7.000ml @11:05:10.67 CSV.dial CTV @11:05:10.69
CTV.dial CSV @11:05:12.00 CBV.dial :waste1 @11:05:12.46 CS.seek 0.000ml @11:05:17.17 AFV.select :carrierSolution @11:05:17.27 delay 2 seconds @11:05:19.28 AS.reconfigure ASconfig @11:05:19.31 AS.seek 1.000ml @11:05:28.89
Flushing lower manifold's waste2b port @11:05:28.99 CSV.dial :flush @11:05:29.79 CS.seek 5.000ml @11:05:41.82 delay 5 seconds @11:05:46.83 CSV.dial :air @11:05:47.37 CS.seek 7.000ml @11:05:48.76 delay 1 second @11:05:49.77 AFV.select :holdingCoil @11:05:49.86 delay 1 second @11:05:50.87 ATV.dial :waste @11:05:52.13 AS.reconfigure ASnorm @11:05:52.16 AS.seek empty @11:05:52.82 CSV.dial CTV @11:05:52.84
CTV.dial CSV @11:05:53.28 CBV.dial :waste2b @11:05:54.02 CS.seek 0.000ml @11:06:10.48 Disinfecting external Intake with kill @11:06:10.57 CSV.dial CSR @11:06:10.99 CSR.select :kill @11:06:11.09 CS.seek 8.000ml @11:06:30.04 delay 5 seconds @11:06:35.06 CSR.select :air @11:06:35.15 CS.seek 10.000ml @11:06:40.52 CSV.dial CBV @11:06:40.54
CBV.dial CSV @11:06:41.56 CTV.dial :intake @11:06:42.27 Intake.select :open @11:06:42.36 CS.seek 2.000ml @11:06:43.66 AFV.select :carrierSolution @11:06:43.77 delay 2 seconds @11:06:45.78 AS.reconfigure ASconfig @11:06:45.81 AS.seek 0.500ml @11:07:01.96
Intake.select :close @11:07:02.06 CTV.dial :air @11:07:02.79 CS.reconfigure CSconfig @11:07:02.82 CS.seek 0.000ml @11:07:08.73 CSV.dial CSR @11:07:09.79 CSR.select :flush @11:07:09.89 CS.seek 5.000ml @11:07:15.30 delay 1 second @11:07:16.31 AFV.select :holdingCoil @11:07:16.41 Building Slug @11:07:16.42 ATV.dial :ARV @11:07:17.67 delay 2 seconds @11:07:19.68 ARV.dial :air1 @11:07:20.00 delay 2 seconds @11:07:22.06 AS.reconfigure ASslow2 @11:07:22.09 AS.seek 0.506ml @11:07:23.36
delay 5 seconds @11:07:28.37 CSR.select :air @11:07:28.45 delay 2 seconds @11:07:28.48
CS.seek 10.000ml @11:07:30.46 ARV.dial :MM2 @11:07:31.80 delay 2 seconds @11:07:33.81 Adding MM2 @11:07:33.87 AS.seek 0.524ml @11:07:41.95
CSV.dial CBV @11:07:43.01 CS.seek 0.000ml @11:07:52.40 delay 25 seconds @11:08:09.04
Garaging FlushPuck in Collection Clamp @11:08:09.10 Elbow.reconfigure TightElbow @11:08:09.13 Elbow.seek CC @11:08:09.24 Elbow.reconfigure LooseElbow @11:08:09.32 Forearm.seek CC @11:08:10.31 CC.seek sealed @11:08:11.23 Hand.grip @11:08:11.91 CC.seek open @11:08:15.23 Forearm.seek retract @11:08:16.30 Elbow.reconfigure TightElbow @11:08:16.34 Elbow.seek garage @11:08:17.41 ARV.dial :PP4 @11:08:17.77 Adding PP4 @11:08:17.78 delay 2 seconds @11:08:19.16
Elbow.reconfigure LooseElbow @11:08:19.24 Forearm.reconfigure TightForearm @11:08:19.27 Forearm.seek garage @11:08:19.83 AS.seek 0.530ml @11:08:20.47
Hand.release @11:08:21.20 Forearm.reconfigure LooseForearm @11:08:21.23 Forearm.seek retract @11:08:26.34 delay 10 seconds @11:08:36.35 ARV.dial :air3 @11:08:36.78 adding ARV air @11:08:36.79 delay 2 seconds @11:08:38.85 AS.seek 0.536ml @11:08:45.29 delay 5 seconds @11:08:50.30 ARV.dial :carrier @11:08:51.34 delay 7 seconds @11:08:58.40 AS.seek 0.594ml @11:09:57.93 delay 5 seconds @11:10:02.94 ATV.dial :ASV @11:10:03.26 delay 2 seconds @11:10:05.32 AS.seek 0.600ml @11:10:11.59 delay 4 seconds @11:10:15.60 ATV.dial ARV @11:10:16.04 delay 2 seconds @11:10:18.05 ARV.dial :carrier @11:10:18.06 delay 2 seconds @11:10:20.12 AS.seek 0.650ml @11:11:11.32 delay 4 seconds @11:11:15.33 ATV.dial PCR @11:11:15.85 ASV.dial :waste1 @11:11:16.52 ARV.dial :air1 @11:11:16.85 Positioning Slug by 0.324ml @11:11:16.90 AS.seek 0.326ml @11:16:47.45 delay 5 seconds @11:16:52.46 ATV.dial ARV @11:16:52.98 ASV.dial :PCRlock @11:16:53.31 delay 5 seconds @11:16:58.32 Running HF183em ProfileEM60C2 thermocycling @11:16:58.33 PCR.program ProfileEM60C2 @11:16:58.44 ProfileEM60C2 should complete in < 2:11:38 @11:16:58.45 PCR.run 2:49:32.5 @13:13:37.95 PCR.off @13:13:38.48 PCR for HF183em completed -- closed /var/log/esp/HF183em14mar1011h224ml.pcr @13:13:38.53 Cleaning PCR AFV.select :carrierSolution @13:13:38.63 delay 2 seconds @13:13:40.64 AS.reconfigure ASconfig @13:13:40.67 AS.seek 0.500ml @13:13:51.09 delay 1 second @13:13:52.10 AFV.select :holdingCoil @13:13:52.19 ASV.dial :waste1 @13:13:52.93 ATV.dial :air3 @13:13:53.86 AS.reconfigure ASslow1 @13:13:53.90 AS.seek 0.510ml @13:13:56.25 delay 2 seconds @13:13:58.26 ATV.dial :bleach @13:13:58.65 AS.seek 0.810ml @13:15:00.12 delay 3 seconds @13:15:03.13 ATV.dial :air3 @13:15:03.48 delay 3 seconds @13:15:06.54 AS.seek 0.835ml @13:15:11.90 delay 3 seconds @13:15:14.91 ATV.dial :bleach @13:15:15.40 AS.seek 0.820ml @13:15:18.70 delay 3 seconds @13:15:21.71 ARV.dial :waste @13:15:22.21 delay 3 seconds @13:15:25.22 ATV.dial :ARV @13:15:25.95 AS.reconfigure ASconfig @13:15:25.99 AS.seek 0.470ml @13:15:46.68 AFV.select :carrierSolution @13:15:46.77 delay 2 seconds @13:15:48.78 AS.seek 1.000ml @13:16:19.96 delay 1 second @13:16:20.97 AFV.select :holdingCoil @13:16:21.06 delay 1 second @13:16:22.07 AS.reconfigure ASfast @13:16:22.10 AS.seek 0.000ml @13:16:49.83 AFV.select :carrierSolution @13:16:49.93 delay 2 seconds @13:16:51.94 AS.reconfigure ASconfig @13:16:51.97 AS.seek 1.000ml @13:17:50.52 delay 1 second @13:17:51.53 AFV.select :holdingCoil @13:17:51.62 delay 1 second @13:17:52.63 AS.reconfigure ASfast @13:17:52.66 AS.seek 0.000ml @13:18:20.39 AFV.select :carrierSolution @13:18:20.48 delay 2 seconds @13:18:22.49 AS.reconfigure ASconfig @13:18:22.52 AS.seek 1.000ml @13:19:21.14 delay 1 second @13:19:22.15 AFV.select :holdingCoil @13:19:22.38 delay 1 second @13:19:23.39 AS.reconfigure ASfast @13:19:23.42 AS.seek 0.000ml @13:19:51.15 ARV.dial between carrier and air1 @13:19:51.56 ATV.dial :air3 @13:19:52.48 AS.reconfigure ASslow1 @13:19:52.52 AS.seek 0.010ml @13:19:54.86 delay 2 seconds @13:19:56.87 ATV.dial :bleach @13:19:57.25 AS.seek 0.810ml @13:22:40.63 delay 3 seconds @13:22:43.64 ATV.dial :air3 @13:22:43.98 delay 3 seconds @13:22:47.04 AS.seek 0.835ml @13:22:52.40 delay 3 seconds @13:22:55.41 ATV.dial :bleach @13:22:55.82 AS.seek 0.820ml @13:22:59.08 delay 3 seconds @13:23:02.09 ATV.dial PCR @13:23:02.40 AS.reconfigure ASconfig @13:23:03.05 AS.seek empty @13:23:51.16 PCR.program :bleachProfile @13:23:51.21 PCR program should complete in < 2:45 @13:23:51.22 PCR.run 10:00 @13:26:49.41 PCR.off @13:26:49.44 AFV.select :carrierSolution @13:26:49.53 delay 2 seconds @13:26:51.54 AS.seek 1.000ml @13:27:50.20 delay 1 second @13:27:51.21 AFV.select :holdingCoil @13:27:51.30 delay 1 second @13:27:52.31 AS.reconfigure ASfast @13:27:52.34 AS.seek 0.000ml @13:28:20.07 delay 3 seconds @13:28:23.08 AFV.select :carrierSolution @13:28:23.17 delay 2 seconds @13:28:25.18 AS.reconfigure ASconfig @13:28:25.21 AS.seek 1.000ml @13:29:23.82 delay 1 second @13:29:24.83 AFV.select :holdingCoil @13:29:24.92 delay 1 second @13:29:25.93 AS.reconfigure ASfast @13:29:25.96 AS.seek 0.000ml @13:29:53.68 delay 3 seconds @13:29:56.69 AFV.select :carrierSolution @13:29:56.78 delay 2 seconds @13:29:58.79 AS.reconfigure ASconfig @13:29:58.82 AS.seek 1.000ml @13:30:57.44 delay 1 second @13:30:58.45 AFV.select :holdingCoil @13:30:58.54 delay 1 second @13:30:59.55 AS.reconfigure ASfast @13:30:59.59 AS.seek 0.000ml @13:31:27.34 delay 3 seconds @13:31:30.35 AFV.select :carrierSolution @13:31:30.44 delay 2 seconds @13:31:32.45 AS.reconfigure ASconfig @13:31:32.48 AS.seek 1.000ml @13:32:31.06 delay 1 second @13:32:32.07 AFV.select :holdingCoil @13:32:32.16 delay 1 second @13:32:33.17 AS.reconfigure ASfast @13:32:33.20 AS.seek 0.000ml @13:33:00.90 delay 3 seconds @13:33:03.91 AFV.select :carrierSolution @13:33:04.00 delay 2 seconds @13:33:06.01 AS.reconfigure ASconfig @13:33:06.04 AS.seek 1.000ml @13:34:04.59 delay 1 second @13:34:05.60 AFV.select :holdingCoil @13:34:05.69 delay 1 second @13:34:06.70 AS.reconfigure ASnorm @13:34:06.73 AS.seek 0.000ml @13:34:58.18 Setting up PCR for ENTap -- writing /var/log/esp/ENTap14mar1013h224ml.pcr @13:34:58.19 Building PCR Slug from ASV with MM1 mix, PP1 primer @13:34:58.20 Priming with carrier @13:34:58.21 ATV.dial ARV @13:34:58.81 ARV.dial :carrier @13:34:59.11 AS.seek 0.500ml @13:35:24.96 AFV.select :carrierSolution @13:35:25.07 delay 2 seconds @13:35:27.08 AS.reconfigure ASconfig @13:35:27.11 AS.seek 1.000ml @13:35:56.57 delay 1 second @13:35:57.58 AFV.select :holdingCoil @13:35:57.68 delay 1 second @13:35:58.69 ATV.dial :waste @13:35:59.95 AS.reconfigure ASnorm @13:35:59.98 AS.seek empty @13:36:51.44 AFV.select :carrierSolution @13:36:51.54 delay 2 seconds @13:36:53.55 AS.reconfigure ASconfig @13:36:53.58 AS.seek 0.500ml @13:37:23.05 delay 1 second @13:37:24.06 AFV.select :holdingCoil @13:37:24.15 Building Slug @13:37:24.16 ATV.dial :ARV @13:37:25.41 delay 2 seconds @13:37:27.42 ARV.dial :air1 @13:37:27.75 delay 2 seconds @13:37:29.81 AS.reconfigure ASslow2 @13:37:29.84 AS.seek 0.506ml @13:37:36.16 delay 2 seconds @13:37:38.17 ARV.dial :MM1 @13:37:38.75 delay 2 seconds @13:37:40.76 Adding MM1 @13:37:40.82 AS.seek 0.524ml @13:37:59.32 delay 25 seconds @13:38:24.33 ARV.dial :PP1 @13:38:24.65 Adding PP1 @13:38:24.66 delay 2 seconds @13:38:26.71 AS.seek 0.530ml @13:38:33.08 delay 10 seconds @13:38:43.09 ARV.dial :air1 @13:38:43.42 adding ARV air @13:38:43.43 delay 2 seconds @13:38:45.48 AS.seek 0.536ml @13:38:52.13 delay 5 seconds @13:38:57.14 ARV.dial :carrier @13:38:57.45 delay 7 seconds @13:39:04.51 AS.seek 0.594ml @13:40:04.04 delay 5 seconds @13:40:09.05 ATV.dial :ASV @13:40:09.46 delay 2 seconds @13:40:11.52 AS.seek 0.600ml @13:40:17.81 delay 4 seconds @13:40:21.82 ATV.dial ARV @13:40:22.15 delay 2 seconds @13:40:24.16 ARV.dial :carrier @13:40:24.17 delay 2 seconds @13:40:26.22 AS.seek 0.650ml @13:41:17.55 delay 4 seconds @13:41:21.56 ATV.dial PCR @13:41:22.05 ASV.dial :waste1 @13:41:22.06 ARV.dial :air1 @13:41:22.41 Positioning Slug by 0.324ml @13:41:22.46 AS.seek 0.326ml @13:46:53.01 delay 5 seconds @13:46:58.02 ATV.dial ARV @13:46:58.54 ASV.dial :PCRlock @13:46:58.98 delay 5 seconds @13:47:03.99 Running ENTap Profile60C thermocycling @13:47:04.00 PCR.program Profile60C @13:47:04.67 Profile60C should complete in < 1:39:08 @13:47:04.68 PCR.run 2:08:55 @15:11:15.07 PCR.off @15:11:15.58 PCR for ENTap completed -- closed /var/log/esp/ENTap14mar1013h224ml.pcr @15:11:15.63 Cleaning PCR @15:11:15.64 AFV.select :carrierSolution @15:11:15.73 delay 2 seconds @15:11:17.74 AS.reconfigure ASconfig @15:11:17.77 AS.seek 0.500ml @15:11:28.20 delay 1 second @15:11:29.21 AFV.select :holdingCoil @15:11:29.30 ASV.dial :waste1 @15:11:29.75 ATV.dial :air3 @15:11:30.68 AS.reconfigure ASslow1 @15:11:30.72 AS.seek 0.510ml @15:11:33.03 delay 2 seconds @15:11:35.04 ATV.dial :bleach @15:11:35.53 AS.seek 0.810ml @15:12:36.98 delay 3 seconds @15:12:39.99 ATV.dial :air3 @15:12:40.32 delay 3 seconds @15:12:43.38 AS.seek 0.835ml @15:12:48.73 delay 3 seconds @15:12:51.74 ATV.dial :bleach @15:12:52.14 AS.seek 0.820ml @15:12:55.43 delay 3 seconds @15:12:58.44 ARV.dial :waste @15:12:59.02 delay 3 seconds @15:13:02.03 ATV.dial :ARV @15:13:02.75 AS.reconfigure ASconfig @15:13:02.79 AS.seek 0.470ml @15:13:23.46 AFV.select :carrierSolution @15:13:23.55 delay 2 seconds @15:13:25.56 AS.seek 1.000ml @15:13:56.79 delay 1 second @15:13:57.80 AFV.select :holdingCoil @15:13:57.89 delay 1 second @15:13:58.90 AS.reconfigure ASfast @15:13:58.93 AS.seek 0.000ml @15:14:26.66 AFV.select :carrierSolution @15:14:26.75 delay 2 seconds @15:14:28.76 AS.reconfigure ASconfig @15:14:28.79 AS.seek 1.000ml @15:15:27.39 delay 1 second @15:15:28.40 AFV.select :holdingCoil @15:15:28.49 delay 1 second @15:15:29.50 AS.reconfigure ASfast @15:15:29.53 AS.seek 0.000ml @15:15:57.27 AFV.select :carrierSolution @15:15:57.36 delay 2 seconds @15:15:59.37 AS.reconfigure ASconfig @15:15:59.40 AS.seek 1.000ml @15:16:58.02 delay 1 second @15:16:59.03 AFV.select :holdingCoil @15:16:59.12 delay 1 second @15:17:00.74 AS.reconfigure ASfast @15:17:00.77 AS.seek 0.000ml @15:17:28.50 ARV.dial between carrier and air1 @15:17:28.91 ATV.dial :air3 @15:17:29.85 AS.reconfigure ASslow1 @15:17:29.88 AS.seek 0.010ml @15:17:32.23 delay 2 seconds @15:17:34.24 ATV.dial :bleach @15:17:34.61 AS.seek 0.810ml @15:20:17.99 delay 3 seconds @15:20:21.00 ATV.dial :air3 @15:20:21.32 delay 3 seconds @15:20:24.38 AS.seek 0.835ml @15:20:29.73 delay 3 seconds @15:20:32.74 ATV.dial :bleach @15:20:33.12 AS.seek 0.820ml @15:20:36.43 delay 3 seconds @15:20:39.44 ATV.dial PCR @15:20:39.78 AS.reconfigure ASconfig @15:20:39.81 AS.seek empty @15:21:27.88 PCR.program :bleachProfile @15:21:27.93 PCR program should complete in < 2:45 @15:21:27.94 PCR.run 10:00 @15:24:26.14 PCR.off @15:24:26.17 AFV.select :carrierSolution @15:24:26.26 delay 2 seconds @15:24:28.27 AS.seek 1.000ml @15:25:26.88 delay 1 second @15:25:27.89 AFV.select :holdingCoil @15:25:27.98 delay 1 second @15:25:28.99 AS.reconfigure ASfast @15:25:29.02 AS.seek 0.000ml @15:25:56.76 delay 3 seconds @15:25:59.77 AFV.select :carrierSolution @15:25:59.86 delay 2 seconds @15:26:01.87 AS.reconfigure ASconfig @15:26:01.90 AS.seek 1.000ml @15:27:00.52 delay 1 second @15:27:01.53 AFV.select :holdingCoil @15:27:01.62 delay 1 second @15:27:02.63 AS.reconfigure ASfast @15:27:02.66 AS.seek 0.000ml @15:27:30.41 delay 3 seconds @15:27:33.42 AFV.select :carrierSolution @15:27:33.51 delay 2 seconds @15:27:35.52 AS.reconfigure ASconfig @15:27:35.55 AS.seek 1.000ml @15:28:34.14 delay 1 second @15:28:35.15 AFV.select :holdingCoil @15:28:35.24 delay 1 second @15:28:36.25 AS.reconfigure ASfast @15:28:36.28 AS.seek 0.000ml @15:29:04.03 delay 3 seconds @15:29:07.04 AFV.select :carrierSolution @15:29:07.13 delay 2 seconds @15:29:09.14 AS.reconfigure ASconfig @15:29:09.17 AS.seek 1.000ml @15:30:07.78 delay 1 second @15:30:08.79 AFV.select :holdingCoil @15:30:08.88 delay 1 second @15:30:09.89 AS.reconfigure ASfast @15:30:09.92 AS.seek 0.000ml @15:30:37.65 delay 3 seconds @15:30:40.66 AFV.select :carrierSolution @15:30:40.75 delay 2 seconds @15:30:42.76 AS.reconfigure ASconfig @15:30:42.79 AS.seek 1.000ml @15:31:41.40 delay 1 second @15:31:42.41 AFV.select :holdingCoil @15:31:42.50 delay 1 second @15:31:43.51 AS.reconfigure ASnorm @15:31:43.54 AS.seek 0.000ml @15:32:35.01 Setting up PCR for ENTem -- writing /var/log/esp/ENTem14mar1015h224ml.pcr @15:32:35.02 Building PCR Slug from ASV with MM2 mix, PP3 primer @15:32:35.03 Priming with carrier @15:32:35.04 ATV.dial ARV @15:32:35.54 ARV.dial :carrier @15:32:35.83 AS.seek 0.500ml @15:33:01.74 AFV.select :carrierSolution @15:33:01.83 delay 2 seconds @15:33:03.84 AS.reconfigure ASconfig @15:33:03.88 AS.seek 1.000ml @15:33:33.34 delay 1 second @15:33:34.35 AFV.select :holdingCoil @15:33:34.44 delay 1 second @15:33:35.45 ATV.dial :waste @15:33:36.78 AS.reconfigure ASnorm @15:33:36.81 AS.seek empty @15:34:28.27 AFV.select :carrierSolution @15:34:28.37 delay 2 seconds @15:34:30.38 AS.reconfigure ASconfig @15:34:30.41 AS.seek 0.500ml @15:34:59.90 delay 1 second @15:35:00.91 AFV.select :holdingCoil @15:35:01.00 Building Slug @15:35:01.01 ATV.dial :ARV @15:35:02.33 delay 2 seconds @15:35:04.34 ARV.dial :air1 @15:35:04.66 delay 2 seconds @15:35:06.72 AS.reconfigure ASslow2 @15:35:06.75 AS.seek 0.506ml @15:35:13.08 delay 2 seconds @15:35:15.09 ARV.dial :MM2 @15:35:16.38 delay 2 seconds @15:35:18.39 Adding MM2 @15:35:18.45 AS.seek 0.524ml @15:35:36.92 delay 25 seconds @15:36:01.92 ARV.dial :PP3 @15:36:02.36 Adding PP3 @15:36:02.37 delay 2 seconds @15:36:04.42 AS.seek 0.530ml @15:36:10.67 delay 10 seconds @15:36:20.68 ARV.dial :air2 @15:36:21.01 adding ARV air delay 2 seconds @15:36:23.07 AS.seek 0.536ml @15:36:29.53 delay 5 seconds @15:36:34.54 ARV.dial :carrier @15:36:35.56 delay 7 seconds @15:36:42.62 AS.seek 0.594ml @15:37:42.22 delay 5 seconds @15:37:47.23 ATV.dial :ASV @15:37:47.70 delay 2 seconds @15:37:50.33 AS.seek 0.600ml @15:37:56.65 delay 4 seconds @15:38:00.66 ATV.dial ARV @15:38:00.98 delay 2 seconds @15:38:02.99 ARV.dial :carrier @15:38:03.00 delay 2 seconds @15:38:05.05 AS.seek 0.650ml @15:38:56.39 delay 4 seconds @15:39:00.40 ATV.dial PCR @15:39:00.90 ASV.dial :waste1 @15:39:00.91 ARV.dial :air1 @15:39:01.26 Positioning Slug by 0.324ml @15:39:01.31 AS.seek 0.326ml @15:44:31.95 delay 5 seconds @15:44:36.96 ATV.dial ARV @15:44:37.48 ASV.dial :PCRlock @15:44:38.09 delay 5 seconds @15:44:43.10 Running ENTem ProfileEM60C thermocycling @15:44:43.11 PCR.program ProfileEM60C @15:44:43.22 ProfileEM60C should complete in < 2:11:38 @15:44:43.23 PCR.run 2:49:32.5 @17:42:22.00 PCR.off @17:42:22.53 PCR for ENTem completed -- closed /var/log/esp/ENTem14mar1015h224ml.pcr @17:42:22.58 Cleaning PCR @17:42:22.59 AFV.select :carrierSolution @17:42:22.68 delay 2 seconds @17:42:24.69 AS.reconfigure ASconfig @17:42:24.72 AS.seek 0.500ml @17:42:35.13 delay 1 second @17:42:36.14 AFV.select :holdingCoil @17:42:36.23 ASV.dial :waste1 @17:42:36.67 ATV.dial :air3 @17:42:37.62 AS.reconfigure ASslow1 @17:42:37.65 AS.seek 0.510ml @17:42:39.96 delay 2 seconds @17:42:41.97 ATV.dial :bleach @17:42:42.45 AS.seek 0.810ml @17:43:43.87 delay 3 seconds @17:43:46.88 ATV.dial :air3 @17:43:47.21 delay 3 seconds @17:43:50.27 AS.seek 0.835ml @17:43:55.60 delay 3 seconds @17:43:58.61 ATV.dial :bleach @17:43:59.01 AS.seek 0.820ml @17:44:02.32 delay 3 seconds @17:44:05.94 ARV.dial :waste @17:44:06.44 delay 3 seconds @17:44:09.45 ATV.dial :ARV @17:44:10.26 AS.reconfigure ASconfig @17:44:10.29 AS.seek 0.470ml @17:44:30.96 AFV.select :carrierSolution @17:44:31.05 delay 2 seconds @17:44:33.06 AS.seek 1.000ml @17:45:04.24 delay 1 second @17:45:05.25 AFV.select :holdingCoil @17:45:05.34 delay 1 second @17:45:06.35 AS.reconfigure ASfast @17:45:06.38 AS.seek 0.000ml @17:45:34.11 AFV.select :carrierSolution @17:45:34.21 delay 2 seconds @17:45:36.22 AS.reconfigure ASconfig @17:45:36.25 AS.seek 1.000ml @17:46:34.83 delay 1 second @17:46:35.84 AFV.select :holdingCoil @17:46:35.93 delay 1 second @17:46:36.94 AS.reconfigure ASfast @17:46:36.97 AS.seek 0.000ml @17:47:04.72 AFV.select :carrierSolution @17:47:04.82 delay 2 seconds @17:47:06.83 AS.reconfigure ASconfig @17:47:06.86 AS.seek 1.000ml @17:48:05.47 delay 1 second @17:48:06.48 AFV.select :holdingCoil @17:48:06.57 delay 1 second @17:48:07.58 AS.reconfigure ASfast @17:48:07.62 AS.seek 0.000ml @17:48:35.37 ARV.dial between carrier and air1 @17:48:35.78 ATV.dial :air3 @17:48:36.72 AS.reconfigure ASslow1 @17:48:36.75 AS.seek 0.010ml @17:48:39.07 delay 2 seconds @17:48:41.08 ATV.dial :bleach @17:48:41.45 AS.seek 0.810ml @17:51:24.85 delay 3 seconds @17:51:27.86 ATV.dial :air3 @17:51:28.19 delay 3 seconds @17:51:31.25 AS.seek 0.835ml @17:51:36.60 delay 3 seconds @17:51:39.61 ATV.dial :bleach @17:51:39.99 AS.seek 0.820ml @17:51:43.40 delay 3 seconds @17:51:46.41 ATV.dial PCR @17:51:46.75 AS.reconfigure ASconfig @17:51:46.78 AS.seek empty @17:52:34.89 PCR.program :bleachProfile @17:52:34.94 PCR program should complete in < 2:45 @17:52:34.95 PCR.run 10:00 @17:55:33.14 PCR.off @17:55:33.17 AFV.select :carrierSolution @17:55:33.26 delay 2 seconds @17:55:35.27 AS.seek 1.000ml @17:56:33.96 delay 1 second @17:56:34.97 AFV.select :holdingCoil @17:56:35.06 delay 1 second @17:56:36.07 AS.reconfigure ASfast @17:56:36.10 AS.seek 0.000ml @17:57:03.85 delay 3 seconds @17:57:06.86 AFV.select :carrierSolution @17:57:06.95 delay 2 seconds @17:57:08.96 AS.reconfigure ASconfig @17:57:08.99 AS.seek 1.000ml @17:58:07.60 delay 1 second @17:58:08.61 AFV.select :holdingCoil @17:58:08.70 delay 1 second @17:58:09.71 AS.reconfigure ASfast @17:58:09.74 AS.seek 0.000ml @17:58:37.44 delay 3 seconds @17:58:40.45 AFV.select :carrierSolution @17:58:40.54 delay 2 seconds @17:58:42.55 AS.reconfigure ASconfig @17:58:42.59 AS.seek 1.000ml @17:59:41.17 delay 1 second @17:59:42.18 AFV.select :holdingCoil @17:59:42.27 delay 1 second @17:59:43.28 AS.reconfigure ASfast @17:59:43.31 AS.seek 0.000ml @18:00:11.06 delay 3 seconds @18:00:14.07 AFV.select :carrierSolution @18:00:14.16 delay 2 seconds @18:00:16.17 AS.reconfigure ASconfig @18:00:16.20 AS.seek 1.000ml @18:01:14.79 delay 1 second @18:01:15.80 AFV.select :holdingCoil @18:01:15.89 delay 1 second @18:01:16.90 AS.reconfigure ASfast @18:01:16.93 AS.seek 0.000ml @18:01:44.67 delay 3 seconds @18:01:47.68 AFV.select :carrierSolution @18:01:47.77 delay 2 seconds @18:01:49.78 AS.reconfigure ASconfig @18:01:49.81 AS.seek 1.000ml @18:02:48.41 delay 1 second @18:02:49.42 AFV.select :holdingCoil @18:02:49.51 delay 1 second @18:02:50.52 AS.reconfigure ASnorm @18:02:50.56 AS.seek 0.000ml @18:03:42.04 Setting up PCR for IPCap -- writing /var/log/esp/IPCap14mar1018h224ml.pcr @18:03:42.05 Building PCR Slug from ASV with MM1 mix, PP2 primer @18:03:42.06 Priming with carrier @18:03:42.07 ATV.dial ARV @18:03:42.57 ARV.dial :carrier @18:03:42.85 AS.seek 0.500ml @18:04:08.72 AFV.select :carrierSolution @18:04:08.81 delay 2 seconds @18:04:10.82 AS.reconfigure ASconfig @18:04:10.85 AS.seek 1.000ml @18:04:40.29 delay 1 second @18:04:41.30 AFV.select :holdingCoil @18:04:41.39 delay 1 second @18:04:42.40 ATV.dial :waste @18:04:43.70 AS.reconfigure ASnorm @18:04:43.73 AS.seek empty @18:05:35.21 AFV.select :carrierSolution @18:05:35.30 delay 2 seconds @18:05:37.31 AS.reconfigure ASconfig @18:05:37.49 AS.seek 0.500ml @18:06:06.95 delay 1 second @18:06:07.96 AFV.select :holdingCoil @18:06:08.05 Building Slug @18:06:08.06 ATV.dial :ARV @18:06:09.30 delay 2 seconds @18:06:11.31 ARV.dial :air1 @18:06:11.64 delay 2 seconds @18:06:13.70 AS.reconfigure ASslow2 @18:06:13.73 AS.seek 0.506ml @18:06:20.22 delay 2 seconds @18:06:22.80 ARV.dial :MM1 @18:06:23.39 delay 2 seconds @18:06:25.40 Adding MM1 @18:06:25.45 AS.seek 0.524ml @18:06:43.98 delay 25 seconds @18:07:08.99 ARV.dial :PP2 @18:07:09.42 Adding PP2 @18:07:09.43 delay 2 seconds @18:07:11.49 AS.seek 0.530ml @18:07:17.91 delay 10 seconds @18:07:27.92 ARV.dial :air2 @18:07:28.25 adding ARV air @18:07:28.26 delay 2 seconds @18:07:30.32 AS.seek 0.536ml @18:07:36.61 delay 5 seconds @18:07:41.62 ARV.dial :carrier @18:07:42.65 delay 7 seconds @18:07:49.71 AS.seek 0.594ml @18:08:49.14 delay 5 seconds @18:08:54.15 ATV.dial :ASV @18:08:54.48 delay 2 seconds @18:08:56.54 AS.seek 0.600ml @18:09:03.39 delay 4 seconds @18:09:07.40 ATV.dial ARV @18:09:07.73 delay 2 seconds @18:09:09.74 ARV.dial :carrier @18:09:09.75 delay 2 seconds @18:09:11.80 AS.seek 0.650ml @18:10:02.99 delay 4 seconds @18:10:07.00 ATV.dial PCR @18:10:07.49 ASV.dial :waste1 @18:10:07.50 ARV.dial :air1 @18:10:07.85 Positioning Slug by 0.324ml @18:10:07.90 AS.seek 0.326ml @18:15:38.63 delay 5 seconds @18:15:43.64 ATV.dial ARV @18:15:44.15 ASV.dial :PCRlock @18:15:44.79 delay 5 seconds @18:15:49.80 Running IPCap Profile59C thermocycling @18:15:49.81 PCR.program Profile59C @18:15:49.91 Profile59C should complete in < 1:39:08 @18:15:49.92 PCR.run 2:08:55 @19:40:46.74 PCR.off @19:40:47.87 PCR for IPCap completed -- closed /var/log/esp/IPCap14mar1018h224ml.pcr @19:40:47.92 Cleaning PCR @19:40:47.93 AFV.select :carrierSolution @19:40:48.02 delay 2 seconds @19:40:50.03 AS.reconfigure ASconfig @19:40:50.06 AS.seek 0.500ml @19:41:00.49 delay 1 second @19:41:01.50 AFV.select :holdingCoil @19:41:01.59 ASV.dial :waste1 @19:41:02.27 ATV.dial :air3 @19:41:03.22 AS.reconfigure ASslow1 @19:41:03.25 AS.seek 0.510ml @19:41:05.57 delay 2 seconds @19:41:07.58 ATV.dial :bleach @19:41:08.06 AS.seek 0.810ml @19:42:09.48 delay 3 seconds @19:42:12.49 ATV.dial :air3 @19:42:12.85 delay 3 seconds @19:42:15.91 AS.seek 0.835ml @19:42:21.26 delay 3 seconds @19:42:24.27 ATV.dial :bleach @19:42:24.67 AS.seek 0.820ml @19:42:27.97 delay 3 seconds @19:42:30.98 ARV.dial :waste @19:42:31.47 delay 3 seconds @19:42:34.48 ATV.dial :ARV @19:42:35.29 AS.reconfigure ASconfig @19:42:35.32 AS.seek 0.470ml @19:42:55.99 AFV.select :carrierSolution @19:42:56.09 delay 2 seconds @19:42:58.10 AS.seek 1.000ml @19:43:29.33 delay 1 second @19:43:30.34 AFV.select :holdingCoil @19:43:30.43 delay 1 second @19:43:31.44 AS.reconfigure ASfast @19:43:31.47 AS.seek 0.000ml @19:43:59.17 AFV.select :carrierSolution @19:43:59.27 delay 2 seconds @19:44:01.28 AS.reconfigure ASconfig @19:44:01.31 AS.seek 1.000ml @19:44:59.88 delay 1 second @19:45:00.89 AFV.select :holdingCoil @19:45:00.98 delay 1 second @19:45:01.99 AS.reconfigure ASfast @19:45:02.02 AS.seek 0.000ml @19:45:29.75 AFV.select :carrierSolution @19:45:29.85 delay 2 seconds @19:45:31.86 AS.reconfigure ASconfig @19:45:31.89 AS.seek 1.000ml @19:46:30.50 delay 1 second @19:46:31.51 AFV.select :holdingCoil @19:46:31.60 delay 1 second @19:46:32.61 AS.reconfigure ASfast @19:46:32.64 AS.seek 0.000ml @19:47:00.39 ARV.dial between carrier and air1 @19:47:00.79 ATV.dial :air3 @19:47:01.72 AS.reconfigure ASslow1 @19:47:01.75 AS.seek 0.010ml @19:47:04.08 delay 2 seconds @19:47:06.09 ATV.dial :bleach @19:47:06.47 AS.seek 0.810ml @19:49:49.84 delay 3 seconds @19:49:52.85 ATV.dial :air3 @19:49:53.27 delay 3 seconds @19:49:56.33 AS.seek 0.835ml @19:50:01.68 delay 3 seconds @19:50:04.69 ATV.dial :bleach @19:50:05.18 AS.seek 0.820ml @19:50:08.54 delay 3 seconds @19:50:11.55 ATV.dial PCR @19:50:11.89 AS.reconfigure ASconfig @19:50:11.92 AS.seek empty @19:51:00.05 PCR.program :bleachProfile @19:51:00.11 PCR program should complete in < 2:45 @19:51:00.12 PCR.run 10:00 @19:53:58.31 PCR.off @19:53:58.34 AFV.select :carrierSolution @19:53:58.43 delay 2 seconds @19:54:00.44 AS.seek 1.000ml @19:54:59.11 delay 1 second @19:55:00.12 AFV.select :holdingCoil @19:55:00.21 delay 1 second @19:55:01.22 AS.reconfigure ASfast @19:55:01.25 AS.seek 0.000ml @19:55:29.01 delay 3 seconds @19:55:32.02 AFV.select :carrierSolution @19:55:32.11 delay 2 seconds @19:55:34.12 AS.reconfigure ASconfig @19:55:34.15 AS.seek 1.000ml @19:56:32.77 delay 1 second @19:56:33.78 AFV.select :holdingCoil @19:56:33.87 delay 1 second @19:56:34.88 AS.reconfigure ASfast @19:56:34.91 AS.seek 0.000ml @19:57:02.66 delay 3 seconds @19:57:05.67 AFV.select :carrierSolution @19:57:05.76 delay 2 seconds @19:57:07.77 AS.reconfigure ASconfig @19:57:07.80 AS.seek 1.000ml @19:58:06.38 delay 1 second @19:58:07.39 AFV.select :holdingCoil @19:58:07.63 delay 1 second @19:58:08.64 AS.reconfigure ASfast @19:58:08.67 AS.seek 0.000ml @19:58:36.39 delay 3 seconds @19:58:39.40 AFV.select :carrierSolution @19:58:39.49 delay 2 seconds @19:58:41.50 AS.reconfigure ASconfig @19:58:41.53 AS.seek 1.000ml @19:59:40.11 delay 1 second @19:59:41.12 AFV.select :holdingCoil @19:59:41.21 delay 1 second @19:59:42.22 AS.reconfigure ASfast @19:59:42.26 AS.seek 0.000ml @20:00:10.00 delay 3 seconds @20:00:13.01 AFV.select :carrierSolution @20:00:13.10 delay 2 seconds @20:00:15.11 AS.reconfigure ASconfig @20:00:15.14 AS.seek 1.000ml @20:01:13.71 delay 1 second @20:01:14.72 AFV.select :holdingCoil @20:01:14.81 delay 1 second @20:01:15.82 AS.reconfigure ASnorm @20:01:15.85 AS.seek 0.000ml @20:02:07.30 Setting up PCR for IPCem -- writing /var/log/esp/IPCem14mar1020h224ml.pcr @20:02:07.31 Building PCR Slug from ASV with MM3 mix, PP5 primer @20:02:07.32 Priming with carrier @20:02:07.33 ATV.dial ARV @20:02:07.82 ARV.dial :carrier @20:02:08.67 AS.seek 0.500ml @20:02:34.55 AFV.select :carrierSolution @20:02:34.66 delay 2 seconds @20:02:36.67 AS.reconfigure ASconfig @20:02:36.71 AS.seek 1.000ml @20:03:06.15 delay 1 second @20:03:07.16 AFV.select :holdingCoil @20:03:07.26 delay 1 second @20:03:08.27 ATV.dial :waste @20:03:09.52 AS.reconfigure ASnorm @20:03:09.55 AS.seek empty @20:04:01.02 AFV.select :carrierSolution @20:04:01.12 delay 2 seconds @20:04:03.13 AS.reconfigure ASconfig @20:04:03.17 AS.seek 0.500ml @20:04:32.66 delay 1 second @20:04:33.67 AFV.select :holdingCoil @20:04:33.76 Building Slug @20:04:33.77 ATV.dial :ARV @20:04:35.11 delay 2 seconds @20:04:37.12 ARV.dial :air1 @20:04:37.44 delay 2 seconds @20:04:39.50 AS.reconfigure ASslow2 @20:04:39.53 AS.seek 0.506ml @20:04:45.78 delay 2 seconds @20:04:47.79 ARV.dial :MM3 @20:04:48.64 delay 2 seconds @20:04:50.65 Adding MM3 @20:04:50.70 AS.seek 0.524ml @20:05:09.21 delay 25 seconds @20:05:34.22 ARV.dial :PP5 @20:05:34.53 Adding PP5 @20:05:34.54 delay 2 seconds @20:05:36.60 AS.seek 0.530ml @20:05:43.16 delay 10 seconds @20:05:53.17 ARV.dial :air3 @20:05:53.49 adding ARV air @20:05:53.50 delay 2 seconds @20:05:55.56 AS.seek 0.536ml @20:06:01.86 delay 5 seconds @20:06:06.87 ARV.dial :carrier @20:06:07.91 delay 7 seconds @20:06:14.97 AS.seek 0.594ml @20:07:14.44 delay 5 seconds @20:07:19.45 ATV.dial :ASV @20:07:19.89 delay 2 seconds @20:07:21.95 AS.seek 0.600ml @20:07:28.31 delay 4 seconds @20:07:32.32 ATV.dial ARV @20:07:32.64 delay 2 seconds @20:07:34.65 ARV.dial :carrier @20:07:34.66 delay 2 seconds @20:07:36.71 AS.seek 0.650ml @20:08:28.04 delay 4 seconds @20:08:32.05 ATV.dial PCR @20:08:32.54 ASV.dial :waste1 @20:08:32.56 ARV.dial :air1 @20:08:32.88 Positioning Slug by 0.324ml @20:08:32.93 AS.seek 0.326ml @20:14:03.59 delay 5 seconds @20:14:08.60 ATV.dial ARV @20:14:09.12 ASV.dial :PCRlock @20:14:09.54 delay 5 seconds @20:14:14.55 Running IPCem ProfileEM59C thermocycling @20:14:14.56 PCR.program ProfileEM59C @20:14:14.67 ProfileEM59C should complete in < 2:11:38 @20:14:14.68 PCR.run 2:49:32.5 @22:11:22.84 PCR.off @22:11:23.37 PCR for IPCem completed -- closed /var/log/esp/IPCem14mar1020h224ml.pcr @22:11:23.38 Full MFB Decontamination @22:11:23.39 Decon extraction column and decon AIV @22:11:23.40 SPE.hold 2:46:40,55.0C @22:11:23.42 ASV.dial :SPEdelivery @22:11:24.35 delay 20 seconds @22:11:44.37 AFV.select :carrierSolution @22:11:44.46 delay 2 seconds @22:11:46.47 AS.reconfigure ASconfig @22:11:46.50 AS.seek 0.500ml @22:11:56.94 delay 1 second @22:11:57.95 AFV.select :holdingCoil @22:11:58.04 ATV.dial :air1 @22:11:59.54 AS.reconfigure ASslow1 @22:11:59.57 AS.seek 0.520ml @22:12:03.94 ATV.dial :ASV @22:12:05.21 ASV.dial :SPErecovery @22:12:05.91 AS.seek 0.620ml @22:12:26.49 ATV.dial :waste @22:12:27.95 AS.reconfigure ASnorm @22:12:27.98 AS.seek empty @22:13:00.02 AFV.select :holdingCoil @22:13:00.03 ATV.dial :waste @22:13:00.04 AS.reconfigure ASconfig @22:13:00.08 AS.seek 0.000ml @22:13:00.36 AFV.select :carrierSolution @22:13:00.45 delay 2 seconds @22:13:02.46 AS.seek 0.500ml @22:13:31.88 delay 1 second @22:13:32.89 AFV.select :holdingCoil @22:13:32.98 ATV.dial :air3 @22:13:33.55 AS.reconfigure ASslow1 @22:13:33.58 AS.seek 0.520ml @22:13:37.94 ATV.dial :air3 @22:13:38.00 AS.seek 0.530ml @22:13:40.31 delay 2 seconds @22:13:42.32 ATV.dial :bleach @22:13:42.69 AS.seek 0.710ml @22:14:19.62 delay 3 seconds @22:14:22.63 ATV.dial :air3 @22:14:23.11 delay 3 seconds @22:14:26.17 AS.seek 0.735ml @22:14:31.50 delay 3 seconds @22:14:34.51 ATV.dial :bleach @22:14:34.91 AS.seek 0.720ml @22:14:38.22 delay 3 seconds @22:14:41.23 ATV.dial ASV @22:14:42.11 ASV.dial :SPErecovery @22:14:42.17 AS.seek 0.365ml @22:15:54.81 ATV.dial :waste @22:15:56.35 AS.reconfigure ASnorm @22:15:56.38 AS.seek empty @22:16:15.42 ATV.dial :air3 @22:16:15.98 AS.reconfigure ASslow1 @22:16:16.01 AS.seek 0.009ml @22:16:18.34 delay 2 seconds @22:16:20.35 ATV.dial :bleach @22:16:20.83 AS.seek 0.579ml @22:18:17.35 delay 3 seconds @22:18:20.36 ATV.dial :air3 @22:18:20.68 delay 3 seconds @22:18:23.74 AS.seek 0.604ml @22:18:29.10 delay 3 seconds @22:18:32.11 ATV.dial :bleach @22:18:32.49 AS.seek 0.589ml @22:18:35.74 delay 3 seconds @22:18:38.75 ATV.dial :ASV @22:18:39.62 ASV.dial :wasteR @22:18:40.38 AS.seek 0.000ml @22:20:40.80 delay 1:00 @22:21:40.81 ATV.dial ASV @22:21:40.82 AFV.select :carrierSolution @22:21:40.91 delay 2 seconds @22:21:42.92 AS.reconfigure ASconfig @22:21:42.95 AS.seek 1.000ml @22:22:41.58 delay 1 second @22:22:42.59 AFV.select :holdingCoil @22:22:42.68 delay 1 second @22:22:43.69 ASV.dial :wasteR @22:22:43.70 AS.reconfigure ASslow1 @22:22:43.73 AS.seek 0.000ml @22:26:07.98 delay 10 seconds @22:26:17.99 ATV.dial :air3 @22:26:19.20 AS.seek 0.010ml @22:26:21.54 delay 2 seconds @22:26:23.55 ATV.dial :bleach @22:26:23.93 AS.seek 0.580ml @22:28:20.47 delay 3 seconds @22:28:23.48 ATV.dial :air3 @22:28:23.89 delay 3 seconds @22:28:26.95 AS.seek 0.605ml @22:28:32.31 delay 3 seconds @22:28:35.32 ATV.dial :bleach @22:28:35.70 AS.seek 0.590ml @22:28:39.00 delay 3 seconds @22:28:42.01 ATV.dial ASV @22:28:42.89 ASV.dial :SPEdelivery @22:28:43.96 AS.seek 0.000ml @22:30:44.67 delay 1:00 @22:31:44.68 AFV.select :carrierSolution @22:31:44.77 delay 2 seconds @22:31:46.78 AS.reconfigure ASconfig @22:31:46.81 AS.seek 1.000ml @22:32:45.41 delay 1 second @22:32:46.42 AFV.select :holdingCoil @22:32:46.51 delay 1 second @22:32:47.52 ATV.dial ASV @22:32:47.53 ASV.dial :SPEdelivery @22:32:47.54 AS.reconfigure ASslow1 @22:32:47.57 AS.seek 0.000ml @22:36:11.81 delay 10 seconds @22:36:21.82 ATV.dial :air2 @22:36:22.54 AS.reconfigure ASslow0 @22:36:22.57 AS.seek 0.800ml @22:37:44.51 delay 3 seconds @22:37:47.52 ATV.dial :ASV @22:37:48.34 ASV.dial :SPEdelivery @22:37:48.36 AS.reconfigure ASslow1 @22:37:48.39 AS.seek 0.030ml @22:40:25.71 delay 20 seconds @22:40:45.72 ATV.dial :waste,via => air3 @22:40:47.17 AS.reconfigure ASconfig @22:40:47.20 AS.seek empty @22:40:49.30 SPE.coast @22:40:49.38 Removing Bleach from Mixing Coil @22:40:49.39 AFV.select :carrierSolution @22:40:49.49 delay 2 seconds @22:40:51.50 AS.seek 0.500ml @22:41:20.99 delay 1 second @22:41:22.00 AFV.select :holdingCoil @22:41:22.09 ATV.dial :ASV @22:41:23.55 ASV.dial :SPErecovery @22:41:24.24 AS.reconfigure ASslow1 @22:41:24.27 AS.seek 0.730ml @22:42:11.44 ASV.dial :wasteD @22:42:11.95 AS.reconfigure ASnorm @22:42:11.98 AS.seek 0.000ml @22:42:49.61 AFV.select :carrierSolution @22:42:49.71 delay 2 seconds @22:42:51.72 AS.reconfigure ASconfig @22:42:51.75 AS.seek 0.500ml @22:43:21.19 delay 1 second @22:43:22.20 AFV.select :holdingCoil @22:43:22.29 ATV.dial ASV @22:43:22.30 ASV.dial :SPErecovery @22:43:23.27 AS.reconfigure ASslow0 @22:43:23.30 AS.seek 0.300ml @22:43:44.00 ATV.dial :air2 @22:43:44.86 AS.reconfigure ASslow1 @22:43:44.89 AS.seek 0.320ml @22:43:49.28 ATV.dial ASV @22:43:50.13 AS.reconfigure ASslow0 @22:43:50.16 AS.seek 0.570ml @22:44:15.97 ASV.dial :wasteD @22:44:16.46 AS.reconfigure ASnorm @22:44:16.49 AS.seek 0.000ml @22:44:45.97 AFV.select :carrierSolution @22:44:46.07 delay 2 seconds @22:44:48.08 AS.reconfigure ASconfig @22:44:48.11 AS.seek 0.500ml @22:45:17.53 delay 1 second @22:45:18.54 AFV.select :holdingCoil @22:45:18.63 ATV.dial ASV @22:45:18.64 ASV.dial :SPErecovery @22:45:19.61 AS.reconfigure ASslow0 @22:45:19.64 AS.seek 0.300ml @22:45:40.35 ATV.dial :air2 @22:45:41.10 AS.reconfigure ASslow1 @22:45:41.13 AS.seek 0.320ml @22:45:45.51 ATV.dial ASV @22:45:46.37 AS.reconfigure ASslow0 @22:45:46.41 AS.seek 0.570ml @22:46:12.21 ASV.dial :wasteD @22:46:12.98 AS.reconfigure ASnorm @22:46:13.01 AS.seek 0.000ml @22:46:42.47 ATV.dial :air2 @22:46:43.28 AS.reconfigure ASslow0 @22:46:43.31 AS.seek 0.300ml @22:47:14.24 ATV.dial :ASV @22:47:15.03 ASV.dial :wasteD @22:47:15.05 AS.reconfigure ASconfig @22:47:15.08 AS.seek 0.050ml @22:47:29.92 ATV.dial :waste @22:47:31.45 AS.seek 0.000ml @22:47:34.66 ASV.dial :SPEdelivery @22:47:35.77 deconLysateINT @22:47:35.78 ATV.dial :air3 @22:47:36.43 AS.reconfigure ASslow1 @22:47:36.46 AS.seek 0.010ml @22:47:38.80 delay 2 seconds @22:47:40.81 ATV.dial :bleach @22:47:41.19 AS.seek 0.960ml @22:50:55.15 delay 3 seconds @22:50:58.16 ATV.dial :air3 @22:50:58.48 delay 3 seconds @22:51:01.54 AS.seek 0.985ml @22:51:06.84 delay 3 seconds @22:51:09.85 ATV.dial :bleach @22:51:10.23 AS.seek 0.970ml @22:51:13.54 delay 3 seconds @22:51:16.55 ATV.dial :AIV @22:51:17.71 AIV.dial :coilToMFB @22:51:17.72 AWV.select :waste @22:51:17.73 AFV.select :carrierSolution @22:51:17.82 delay 2 seconds @22:51:19.83 AS.reconfigure ASconfig @22:51:19.86 AS.seek 1.000ml @22:51:21.95 delay 1 second @22:51:22.96 AFV.select :holdingCoil @22:51:23.05 delay 1 second @22:51:24.06 AS.reconfigure ASslow0 @22:51:24.09 AS.seek empty @22:53:06.49 AFV.select :carrierSolution @22:53:06.58 delay 2 seconds @22:53:08.59 AS.reconfigure ASconfig @22:53:08.62 AS.seek 1.000ml @22:54:07.27 delay 1 second @22:54:08.28 AFV.select :holdingCoil @22:54:08.37 delay 1 second @22:54:09.38 AS.reconfigure ASslow0 @22:54:09.41 AS.seek empty @22:55:51.80 AFV.select :carrierSolution @22:55:51.89 delay 2 seconds @22:55:54.47 AS.reconfigure ASconfig @22:55:54.51 AS.seek 1.000ml @22:56:53.16 delay 1 second @22:56:54.17 AFV.select :holdingCoil @22:56:54.26 delay 1 second @22:56:55.27 AS.reconfigure ASslow0 @22:56:55.30 AS.seek empty @22:58:37.68 ATV.dial :air2 @22:58:38.26 AS.reconfigure ASslow1 @22:58:38.29 AS.seek 0.009ml @22:58:40.63 AS.reconfigure ASslow0 @22:58:40.66 AS.seek 1.000ml @23:00:21.99 ATV.dial AIV @23:00:22.61 AS.seek 0.020ml @23:02:02.91 ATV.dial :waste @23:02:04.19 AS.seek 0.000ml @23:02:06.49 AWV.select :air @23:02:06.63 Cleaning PCR @23:02:06.64 AFV.select :carrierSolution @23:02:06.73 delay 2 seconds @23:02:08.74 AS.reconfigure ASconfig @23:02:08.77 AS.seek 0.500ml @23:02:38.21 delay 1 second @23:02:39.23 AFV.select :holdingCoil @23:02:39.32 ASV.dial :waste1 @23:02:39.79 ATV.dial :air3 @23:02:40.44 AS.reconfigure ASslow1 @23:02:40.47 AS.seek 0.510ml @23:02:42.78 delay 2 seconds @23:02:44.79 ATV.dial :bleach @23:02:45.17 AS.seek 0.810ml @23:03:46.64 delay 3 seconds @23:03:49.65 ATV.dial :air3 @23:03:49.98 delay 3 seconds @23:03:53.04 AS.seek 0.835ml @23:03:58.35 delay 3 seconds @23:04:01.36 ATV.dial :bleach @23:04:01.84 AS.seek 0.820ml @23:04:05.15 delay 3 seconds @23:04:08.16 ARV.dial :waste @23:04:08.65 delay 3 seconds @23:04:11.66 ATV.dial :ARV @23:04:12.50 AS.reconfigure ASconfig @23:04:12.53 AS.seek 0.470ml @23:04:33.21 AFV.select :carrierSolution @23:04:33.30 delay 2 seconds @23:04:35.31 AS.seek 1.000ml @23:05:06.49 delay 1 second @23:05:07.50 AFV.select :holdingCoil @23:05:07.59 delay 1 second @23:05:08.60 AS.reconfigure ASfast @23:05:08.63 AS.seek 0.000ml @23:05:36.37 AFV.select :carrierSolution @23:05:36.48 delay 2 seconds @23:05:38.49 AS.reconfigure ASconfig @23:05:38.52 AS.seek 1.000ml @23:06:37.13 delay 1 second @23:06:38.14 AFV.select :holdingCoil @23:06:38.23 delay 1 second @23:06:39.24 AS.reconfigure ASfast @23:06:39.27 AS.seek 0.000ml @23:07:07.02 AFV.select :carrierSolution @23:07:07.12 delay 2 seconds @23:07:09.13 AS.reconfigure ASconfig @23:07:09.16 AS.seek 1.000ml @23:08:07.74 delay 1 second @23:08:08.75 AFV.select :holdingCoil @23:08:08.84 delay 1 second @23:08:09.85 AS.reconfigure ASfast @23:08:09.88 AS.seek 0.000ml @23:08:37.61 ARV.dial between carrier and air1 @23:08:38.13 ATV.dial :air3 @23:08:39.16 AS.reconfigure ASslow1 @23:08:39.19 AS.seek 0.010ml @23:08:41.52 delay 2 seconds @23:08:43.53 ATV.dial :bleach @23:08:44.02 AS.seek 0.810ml @23:11:27.45 delay 3 seconds @23:11:30.46 ATV.dial :air3 @23:11:30.79 delay 3 seconds @23:11:33.85 AS.seek 0.835ml @23:11:39.20 delay 3 seconds @23:11:42.21 ATV.dial :bleach @23:11:42.61 AS.seek 0.820ml @23:11:45.92 delay 3 seconds @23:11:48.93 ATV.dial PCR @23:11:49.26 AS.reconfigure ASconfig @23:11:49.29 AS.seek empty @23:12:37.42 PCR.program :bleachProfile @23:12:37.48 PCR program should complete in < 2:45 @23:12:37.49 PCR.run 10:00 @23:15:41.72 PCR.off @23:15:41.75 AFV.select :carrierSolution @23:15:41.84 delay 2 seconds @23:15:43.85 AS.seek 1.000ml @23:16:42.50 delay 1 second @23:16:43.51 AFV.select :holdingCoil @23:16:43.60 delay 1 second @23:16:44.61 AS.reconfigure ASfast @23:16:44.64 AS.seek 0.000ml @23:17:12.36 delay 3 seconds @23:17:15.37 AFV.select :carrierSolution @23:17:15.46 delay 2 seconds @23:17:17.47 AS.reconfigure ASconfig @23:17:17.50 AS.seek 1.000ml @23:18:16.09 delay 1 second @23:18:17.10 AFV.select :holdingCoil @23:18:17.19 delay 1 second @23:18:18.20 AS.reconfigure ASfast @23:18:18.23 AS.seek 0.000ml @23:18:45.97 delay 3 seconds @23:18:48.98 AFV.select :carrierSolution @23:18:49.07 delay 2 seconds @23:18:51.08 AS.reconfigure ASconfig @23:18:51.11 AS.seek 1.000ml @23:19:49.69 delay 1 second @23:19:50.70 AFV.select :holdingCoil @23:19:50.79 delay 1 second @23:19:51.80 AS.reconfigure ASfast @23:19:51.83 AS.seek 0.000ml @23:20:19.57 delay 3 seconds @23:20:22.58 AFV.select :carrierSolution @23:20:23.25 delay 2 seconds @23:20:25.26 AS.reconfigure ASconfig @23:20:25.29 AS.seek 1.000ml @23:21:23.88 delay 1 second @23:21:24.89 AFV.select :holdingCoil @23:21:24.98 delay 1 second @23:21:25.99 AS.reconfigure ASfast @23:21:26.02 AS.seek 0.000ml @23:21:53.75 delay 3 seconds @23:21:56.76 AFV.select :carrierSolution @23:21:56.85 delay 2 seconds @23:21:58.86 AS.reconfigure ASconfig @23:21:58.89 AS.seek 1.000ml @23:22:57.50 delay 1 second @23:22:58.51 AFV.select :holdingCoil @23:22:58.60 delay 1 second @23:22:59.61 AS.reconfigure ASnorm @23:22:59.64 AS.seek 0.000ml @23:23:51.10 AFV.select :carrierSolution @23:23:51.19 delay 2 seconds @23:23:53.20 AS.reconfigure ASconfig @23:23:53.23 AS.seek 1.000ml @23:24:51.84 delay 1 second @23:24:52.85 AFV.select :holdingCoil @23:24:52.94 delay 1 second @23:24:53.95 ATV.dial :waste @23:24:54.85 AS.reconfigure ASnorm @23:24:54.88 AS.seek 0.500ml @23:25:20.82 ATV.dial :waste2 @23:25:21.91 AS.seek 0.000ml @23:25:47.85
Cleaning PCR @23:25:47.86 AFV.select :carrierSolution @23:25:47.95 delay 2 seconds @23:25:49.96 AS.reconfigure ASconfig @23:25:49.99 AS.seek 0.500ml @23:26:19.40 delay 1 second @23:26:20.41 AFV.select :holdingCoil @23:26:20.50 ASV.dial :waste1 @23:26:20.51 ATV.dial :air3 @23:26:21.98 AS.reconfigure ASslow1 @23:26:22.01 AS.seek 0.510ml @23:26:24.38 delay 2 seconds @23:26:26.39 ATV.dial :bleach @23:26:26.76 AS.seek 0.810ml @23:27:28.19 delay 3 seconds @23:27:31.21 ATV.dial :air3 @23:27:31.54 delay 3 seconds @23:27:34.60 AS.seek 0.835ml @23:27:39.97 delay 3 seconds @23:27:42.99 ATV.dial :bleach @23:27:43.37 AS.seek 0.820ml @23:27:46.71 delay 3 seconds @23:27:49.73 ARV.dial :waste @23:27:50.22 delay 3 seconds @23:27:53.23 ATV.dial :ARV @23:27:53.95 AS.reconfigure ASconfig @23:27:53.99 AS.seek 0.470ml @23:28:14.68 AFV.select :carrierSolution @23:28:14.77 delay 2 seconds @23:28:16.78 AS.seek 1.000ml @23:28:48.02 delay 1 second @23:28:49.03 AFV.select :holdingCoil @23:28:49.12 delay 1 second @23:28:50.13 AS.reconfigure ASfast @23:28:50.16 AS.seek 0.000ml @23:29:17.89 AFV.select :carrierSolution @23:29:17.98 delay 2 seconds @23:29:19.99 AS.reconfigure ASconfig @23:29:20.02 AS.seek 1.000ml @23:30:18.63 delay 1 second @23:30:19.65 AFV.select :holdingCoil @23:30:19.74 delay 1 second @23:30:20.75 AS.reconfigure ASfast @23:30:20.78 AS.seek 0.000ml @23:30:48.52 AFV.select :carrierSolution @23:30:48.61 delay 2 seconds @23:30:50.62 AS.reconfigure ASconfig @23:30:50.65 AS.seek 1.000ml @23:31:49.26 delay 1 second @23:31:50.28 AFV.select :holdingCoil @23:31:50.37 delay 1 second @23:31:51.38 AS.reconfigure ASfast @23:31:51.41 AS.seek 0.000ml @23:32:19.17 ARV.dial between carrier and air1 @23:32:19.67 ATV.dial :air3 @23:32:20.69 AS.reconfigure ASslow1 @23:32:20.72 AS.seek 0.010ml @23:32:23.06 delay 2 seconds @23:32:25.07 ATV.dial :bleach @23:32:25.56 AS.seek 0.810ml @23:35:08.94 delay 3 seconds @23:35:11.95 ATV.dial :air3 @23:35:12.27 delay 3 seconds @23:35:15.33 AS.seek 0.835ml @23:35:20.68 delay 3 seconds @23:35:23.69 ATV.dial :bleach @23:35:24.09 AS.seek 0.820ml @23:35:27.51 delay 3 seconds @23:35:30.52 ATV.dial PCR @23:35:30.84 AS.reconfigure ASconfig @23:35:30.87 AS.seek empty @23:36:18.97 PCR.program :bleachProfile @23:36:19.03 PCR program should complete in < 2:45 @23:36:19.04 PCR.run 10:00 @23:39:23.28 PCR.off @23:39:23.31 AFV.select :carrierSolution @23:39:23.40 delay 2 seconds @23:39:25.41 AS.seek 1.000ml @23:40:24.04 delay 1 second @23:40:25.05 AFV.select :holdingCoil @23:40:25.14 delay 1 second @23:40:26.15 AS.reconfigure ASfast @23:40:26.18 AS.seek 0.000ml @23:40:53.93 delay 3 seconds @23:40:56.94 AFV.select :carrierSolution @23:40:57.03 delay 2 seconds @23:40:59.04 AS.reconfigure ASconfig @23:40:59.07 AS.seek 1.000ml @23:41:57.68 delay 1 second @23:41:58.69 AFV.select :holdingCoil @23:41:58.78 delay 1 second @23:41:59.79 AS.reconfigure ASfast @23:42:00.34 AS.seek 0.000ml @23:42:28.10 delay 3 seconds @23:42:31.11 AFV.select :carrierSolution @23:42:31.20 delay 2 seconds @23:42:33.21 AS.reconfigure ASconfig @23:42:33.25 AS.seek 1.000ml @23:43:31.83 delay 1 second @23:43:32.85 AFV.select :holdingCoil @23:43:32.94 delay 1 second @23:43:33.95 AS.reconfigure ASfast @23:43:33.99 AS.seek 0.000ml @23:44:01.73 delay 3 seconds @23:44:04.74 AFV.select :carrierSolution @23:44:04.83 delay 2 seconds @23:44:06.84 AS.reconfigure ASconfig @23:44:06.87 AS.seek 1.000ml @23:45:05.45 delay 1 second @23:45:06.46 AFV.select :holdingCoil @23:45:06.55 delay 1 second @23:45:07.56 AS.reconfigure ASfast @23:45:07.60 AS.seek 0.000ml @23:45:35.31 delay 3 seconds @23:45:38.32 AFV.select :carrierSolution @23:45:38.41 delay 2 seconds @23:45:40.42 AS.reconfigure ASconfig @23:45:40.45 AS.seek 1.000ml @23:46:39.03 delay 1 second @23:46:40.04 AFV.select :holdingCoil @23:46:40.13 delay 1 second @23:46:41.14 AS.reconfigure ASnorm @23:46:41.17 AS.seek 0.000ml @23:47:32.61 Completed {wqPCR} begun 09:02:36 @23:47:32.64 Asleep til 09:02PDT11-Mar-14 @23:47:32.65 AS.stop @23:47:32.67
Sleeping until 09:02PDT11-Mar-14 @23:47:32.69 GoodNews.email "Then, in tube 2: wqPCR\n/home/esp/esp2local/mission/14mar05sccwrpDeploy.rb:64",Subject => "Asleep until 09:02PDT11-Mar-14" @23:47:32.72 CC.stop @23:47:34.41 CS.stop @23:47:34.43 PC.stop @23:47:34.46 PS.stop @23:47:34.49 Forearm.stop @23:47:34.52 Elbow.stop @23:47:34.55 SS.stop @23:47:34.62 Sleepy.power :raw,:OFF @23:47:34.63 Sleepy.power :dwarf,:OFF Shutdown @23:53:45.89 Session Begun @23:54:20.85 -> Trigger.replace "8AM" @23:54:20.89
Asleep til 8AM @23:54:20.94 Sleeping until 08:00PDT11-Mar-14 @23:54:20.96 GoodNews.email "Then, in tube 2: wqPCR\n/home/esp/esp2local/mission/14mar05sccwrpDeploy.rb:64",Subject => "Asleep until 08:00PDT11-Mar-14" @23:54:21.00 Shutdown @23:54:53.39 -> exit @23:54:53.41 Session Ended @08:00:00.42PDT11-Mar-14
Powering up Dwarves @08:00:05.60 Sleepy.power :raw,:ON @08:00:06.06 SPE.configure SPEconfig @08:00:06.08 AS.configure ASconfig @08:00:06.12 CC.configure CCconfig @08:00:06.15 CS.configure CSconfig @08:00:06.18 PC.configure PCconfig @08:00:06.21 PS.configure PSconfig @08:00:06.24 Forearm.configure LooseForearm @08:00:06.27 Elbow.configure LooseElbow @08:00:06.30 SC.configure SCloose @08:00:06.33 SE.configure SEconfig @08:00:06.36 SS.configure SSconfig @08:00:06.39 CH.configure CHconfig @08:00:06.79 PH.configure PHconfig @08:00:06.89 Valve.reset @08:00:07.10 PCR.off @08:00:07.13 PCR.pidtemp= 50,0.1,0,300,25,25 @08:00:07.16 PCR.caltemp= 6.6661,0.0344,0.0 @08:00:07.26 Intake.select :close @08:00:07.29 Exhaust.select :close CTV.dial :air @08:00:07.34 PSV.dial :air @08:00:07.37
PC.reconfigure PCconfig @08:00:07.42 PC.home.seek open @08:00:07.44 CSV.dial :waste1 @08:00:07.88 SSV.dial :spit,avoiding => exhaust @08:00:07.97 PS.reconfigure PSconfig @08:00:08.01 PS.home.seek 0.000ml @08:00:08.68 CS.reconfigure CSfast @08:00:08.71 SS.reconfigure SSconfig @08:00:08.80 SS.home.seek 0.00ml @08:00:08.82 CS.home.seek 0.000ml @08:00:08.90 CC.reconfigure CCconfig @08:00:08.94 CC.home.seek open @08:00:18.25 PS.coast @08:00:18.33
CC.seek open @08:00:18.35 PC.seek open @08:00:18.49
Forearm.reconfigure LooseForearm @08:00:18.52 Forearm.home.seek retract @08:00:19.29 Hand.grip @08:00:19.31
Elbow.jog -1500 @08:00:19.90 Elbow.reconfigure LooseElbow @08:00:19.94 Elbow.home.seek clear @08:00:24.68 SE.reconfigure SEconfig @08:00:24.71 SE.home.seek down @08:00:25.25 SC.reconfigure SCsloppy @08:00:25.29 SC.home.seek initial @08:00:27.51 Forearm.seek retract @08:00:27.57 Elbow.seek hammer @08:00:28.75 Forearm.reconfigure TightForearm @08:00:28.80 Forearm.seek 2 @08:00:29.88 Forearm.seek retract @08:00:30.96 Forearm.reconfigure LooseForearm @08:00:31.46PDT11-Mar-14 ===> STARTED wqPCR (phase #8) <=== @08:00:31.47 Starting initialPurge @08:00:31.48 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:00:31.53 Hand.release @08:00:32.39 Elbow.reconfigure TightElbow @08:00:32.42 Elbow.seek FlushPuck::Garage @08:00:34.13 Elbow.reconfigure LooseElbow @08:00:34.21 Forearm.seek garage @08:00:35.25 Hand.grip @08:00:36.02 Forearm.seek retract @08:00:37.02 Elbow.reconfigure TightElbow @08:00:37.05 Elbow.seek CC @08:00:39.74 Elbow.reconfigure LooseElbow @08:00:39.83 Forearm.reconfigure TightForearm @08:00:39.86 Forearm.seek CC @08:00:40.97 CC.seek closed @08:00:44.74 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.95 @08:00:44.79 Hand.release @08:00:45.59 Forearm.reconfigure LooseForearm @08:00:45.62 Forearm.seek retract @08:00:46.54 Flushing the Collection Valve::Manifold @08:00:46.63 CS Pulling 5ml of flush @08:00:46.64 CSV.dial CSR @08:00:47.91 CSR.select :flush @08:00:48.01 CS.reconfigure CSconfig @08:00:48.05 CS.seek 5.000ml @08:01:01.44 delay 5 seconds @08:01:06.45 CSR.select :air @08:01:06.59 CS.reconfigure CSfast @08:01:06.62 CS.seek 7.500ml @08:01:13.31 CSV.dial :waste @08:01:14.56 CS.reconfigure CSconfig @08:01:14.60 CS.seek 0.000ml @08:01:34.41 Purging external Intake @08:01:34.50 CSV.dial :flush @08:01:35.15 CS.reconfigure CSfast @08:01:35.18 CS.seek 10.000ml @08:01:59.03 delay 5 seconds @08:02:04.05 CSV.dial CBV @08:02:04.07
CBV.dial CSV @08:02:04.78 CTV.dial :intake @08:02:05.53 Intake.select :open @08:02:05.62 CS.seek 0.000ml @08:02:29.69 Intake.select :close @08:02:29.78 CTV.dial :air @08:02:30.53 Flushing upper manifold @08:02:30.62 CSV.dial :flush @08:02:31.41 CS.seek 5.000ml @08:02:43.84 delay 5 seconds @08:02:48.85 CSV.dial :air @08:02:49.33 CS.seek 7.000ml @08:02:54.81 CSV.dial CBV @08:02:54.83
CBV.dial CSV @08:02:55.90 CTV.dial :air @08:02:55.92 CS.seek 0.000ml @08:03:12.71 delay 7 seconds @08:03:19.72 CS.seek 3.000ml @08:03:27.49 CSV.dial :waste @08:03:28.00 CS.seek 0.000ml @08:03:35.76 CSV.dial :flush @08:03:36.19 CS.seek 3.000ml @08:03:43.99 delay 5 seconds @08:03:48.99 CSV.dial :air @08:03:49.48 CS.seek 5.001ml @08:03:54.87 CSV.dial :air @08:03:54.88 CS.seek 0.000ml @08:04:07.12 delay 7 seconds @08:04:14.14 CS.seek 3.000ml @08:04:21.93 CSV.dial :waste @08:04:22.72 CS.seek 0.000ml @08:04:30.46 Garaging FlushPuck in Collection Clamp @08:04:30.51 Elbow.reconfigure TightElbow @08:04:30.55 Elbow.seek CC @08:04:30.73 Elbow.reconfigure LooseElbow @08:04:30.82 Forearm.seek CC @08:04:31.78 CC.seek sealed @08:04:32.68 Hand.grip @08:04:33.42 CC.seek open @08:04:36.71 Forearm.seek retract @08:04:37.64 Elbow.reconfigure TightElbow @08:04:37.68 Elbow.seek garage @08:04:41.09 Elbow.reconfigure LooseElbow @08:04:41.18 Forearm.reconfigure TightForearm @08:04:41.21 Forearm.seek garage @08:04:42.36 Hand.release @08:04:43.08 Forearm.reconfigure LooseForearm @08:04:43.11 Forearm.seek retract @08:04:44.25 Starting mfb @08:04:44.26 pcrBAC sampling at most 1000ml, wcr at most 1000ml @08:04:44.27 BACfans.email "Can@08:00:31, 14.3C, 70% humidity, 14.2psia, 13.729V, 0.591A, 0.598A avg, 8.12W\nCTD data unavailable\nISUS data unavailable",Subject => "pcrBAC sampling at most 1000ml, wcr at most 1000ml" @08:04:44.30
Loading sh1 puck from tube 2 into Collection Clamp @08:04:46.12 Readying the MFB @08:04:46.13 Homing MFB Syringe @08:04:46.14 AFV.select :holdingCoil @08:04:46.38
SC.reconfigure SCloose @08:04:46.43 ATV.dial :air2 @08:04:46.60
Elbow.reconfigure TightElbow @08:04:46.63 Elbow.seek 2 @08:04:47.75 AS.reconfigure ASconfig @08:04:47.78 AS.home.seek home @08:04:48.57
Forearm.reconfigure TightForearm @08:04:48.61 Forearm.seek 2 + 800 counts @08:04:49.66 SE.reconfigure SEpick @08:04:49.71 SE.seek beamBreak @08:04:52.59 SE.configure SEconfig @08:04:52.71 Elbow.seek 2 @08:04:52.95 Forearm.seek 2 + 300 counts @08:04:53.16 Forearm.reconfigure LooseForearm @08:04:53.20 Hand.grip @08:04:53.93 SE.seek down @08:04:54.10
Forearm.seek retract @08:04:55.09 Elbow.seek CC @08:04:55.27 AFV.select :carrierSolution @08:04:55.37 delay 2 seconds @08:04:56.60
Elbow.reconfigure LooseElbow @08:04:56.69 Forearm.reconfigure TightForearm @08:04:56.72 Forearm.seek CC @08:04:56.74 SE.coast @08:04:57.38 AS.seek 1.000ml @08:04:57.87
CC.seek closed @08:05:01.70 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.93 @08:05:01.71 Hand.release @08:05:02.46 Forearm.reconfigure LooseForearm @08:05:02.49 Forearm.seek retract @08:05:03.42 SC.coast @08:05:03.45 collectSample 1000ml w/bubble point of 55psi Priming 7ml @08:05:03.46 Sleepy.power :sampler,:ON @08:05:03.47 Intake.select :close Exhaust.select :close @08:05:03.48 SS.hold @08:05:03.64 Calibrating pressure and temperature sensors @08:05:03.65 CTV.dial :air @08:05:03.66 SSV.dial :spit,avoiding => exhaust @08:05:03.68 SS.reconfigure SSfast @08:05:03.71 SS.seek 5.00ml @08:05:06.75 SSV.dial CBV @08:05:06.77
CBV.dial SSV @08:05:08.14 delay 3.8 seconds @08:05:12.02 SSV.dial :spit @08:05:13.37 SS.seek 0.00ml @08:05:15.88 SSV.dial CBV @08:05:17.22 CTV.dial :intake @08:05:17.99 Waiting for external sampler to finish priming... @08:05:18.00 delayUntil "08:15:03.46" @08:05:49.05 delay 1 second @08:05:50.06 AFV.select :holdingCoil @08:05:50.15 delay 1 second @08:05:51.71 ATV.dial :waste @08:05:52.62 AS.reconfigure ASnorm @08:05:52.65 AS.home.seek empty @08:06:45.81 ATV.dial :waste @08:06:45.82 ARV.dial between carrier and air1 @08:06:45.89 ASV.dial :waste1 @08:15:03.47 SSV.dial CBV @08:15:03.49
CBV.dial SSV @08:15:03.50 Allowing 55s for 7.0ml of remaining 7.0ml Intake.select :open @08:15:05.63 Intake @52.9psia, vacuum from 20.0 to 24.0psi @08:15:05.64 SS.reconfigure forSampling @08:15:05.68 SS.seek 7.00ml @08:15:11.15 Stroke completed -- waiting for pressure to equalize ... @08:15:11.79 Intake.select :close @08:15:11.92 SSV.dial :exhaust @08:15:12.70 Exhaust.select :open @08:15:12.81 SS.reconfigure SSexhale @08:15:12.85 SS.seek 0.00ml @08:15:16.87 Exhaust.select :close @08:15:16.97 Sampling 1000.0ml @08:15:16.98 Intake.select :close @08:15:16.99 Exhaust.select :close @08:15:17.00 SS.reconfigure SSconfig @08:15:17.03 SS.hold @08:15:17.18 CTV.dial :intake @08:15:17.19 SSV.dial CBV @08:15:17.21
CBV.dial SSV @08:15:17.99 Allowing 185s for 25.0ml of remaining 1000.0ml @08:15:18.00 Intake.select :open @08:15:20.13 Intake @52.1psia, vacuum from 20.0 to 24.0psi @08:15:20.14 SS.reconfigure forSampling @08:15:20.18 SS.seek 25.00ml @08:15:35.03 Stroke completed -- waiting for pressure to equalize ... @08:15:35.67 Intake.select :close @08:15:35.80 SSV.dial :exhaust @08:15:36.57 Exhaust.select :open @08:15:36.67 SS.reconfigure SSexhale @08:15:36.71 SS.seek 0.00ml @08:15:45.95 Exhaust.select :close @08:15:46.04 SSV.dial CBV @08:15:46.06
CBV.dial SSV @08:15:46.82 Allowing 185s for 25.0ml of remaining 975.0ml @08:15:46.83 Intake.select :open @08:15:48.96 Intake @52.4psia, vacuum from 20.0 to 24.0psi @08:15:48.97 SS.reconfigure forSampling @08:15:49.01 SS.seek 25.00ml @08:15:58.45 Vacuum too high -- waiting up to 40s @08:15:58.72 Intake @45.0psia, vacuum from 20.0 to 24.0psi @08:15:58.73 SS.reconfigure forSampling @08:15:58.76 SS.seek 25.00ml @08:16:05.35 Stroke completed -- waiting for pressure to equalize ... @08:16:05.99 Intake.select :close @08:16:06.12 SSV.dial :exhaust @08:16:06.90 Exhaust.select :open @08:16:06.99 SS.reconfigure SSexhale @08:16:07.03 SS.seek 0.00ml @08:16:15.90 Exhaust.select :close @08:16:15.99 SSV.dial CBV @08:16:16.01
CBV.dial SSV @08:16:16.79 Allowing 185s for 25.0ml of remaining 950.0ml @08:16:16.80 Intake.select :open @08:16:18.93 Intake @52.7psia, vacuum from 20.0 to 24.0psi @08:16:18.94 SS.reconfigure forSampling @08:16:18.98 SS.seek 25.00ml @08:16:23.32 Vacuum too high -- waiting up to 40s @08:16:23.59 Intake @45.6psia, vacuum from 20.0 to 24.0psi @08:16:23.60 SS.reconfigure forSampling @08:16:23.63 SS.seek 25.00ml @08:16:37.35 Stroke completed -- waiting for pressure to equalize ... @08:16:37.99 Intake.select :close @08:16:38.13 SSV.dial :exhaust @08:16:38.92 Exhaust.select :open @08:16:39.01 SS.reconfigure SSexhale @08:16:39.05 SS.seek 0.00ml @08:16:47.95 Exhaust.select :close @08:16:48.05 SSV.dial CBV @08:16:48.07
CBV.dial SSV @08:16:48.85 Allowing 185s for 25.0ml of remaining 925.0ml @08:16:48.86 Intake.select :open @08:16:50.99 Intake @51.5psia, vacuum from 20.0 to 24.0psi @08:16:51.00 SS.reconfigure forSampling @08:16:51.04 SS.seek 25.00ml @08:16:54.66 Vacuum too high -- waiting up to 40s @08:16:54.93 Intake @47.4psia, vacuum from 20.0 to 24.0psi @08:16:54.94 SS.reconfigure forSampling @08:16:54.97 SS.seek 25.00ml @08:17:01.47 Vacuum too high -- waiting up to 40s @08:17:01.74 Intake @47.3psia, vacuum from 20.0 to 24.0psi @08:17:01.75 SS.reconfigure forSampling @08:17:01.78 SS.seek 25.00ml @08:17:09.82 Vacuum too high -- waiting up to 40s @08:17:10.09 Intake @48.6psia, vacuum from 20.0 to 24.0psi @08:17:10.10 SS.reconfigure forSampling @08:17:10.13 SS.seek 25.00ml @08:17:14.54 Stroke completed -- waiting for pressure to equalize ... @08:17:15.18 Intake.select :close @08:17:15.31 SSV.dial :exhaust @08:17:16.10 Exhaust.select :open @08:17:16.19 SS.reconfigure SSexhale @08:17:16.23 SS.seek 0.00ml @08:17:25.02 Exhaust.select :close @08:17:25.11 SSV.dial CBV @08:17:25.13
CBV.dial SSV @08:17:25.93 Allowing 185s for 25.0ml of remaining 900.0ml @08:17:25.94 Intake.select :open @08:17:28.07 Intake @50.8psia, vacuum from 20.0 to 24.0psi @08:17:28.08 SS.reconfigure forSampling @08:17:28.12 SS.seek 25.00ml @08:17:30.07 Vacuum too high -- waiting up to 40s @08:17:30.32 Intake @48.5psia, vacuum from 20.0 to 24.0psi @08:17:30.33 SS.reconfigure forSampling @08:17:30.37 SS.seek 25.00ml @08:17:46.31 Vacuum too high -- waiting up to 40s @08:17:46.58 Intake @50.4psia, vacuum from 20.0 to 24.0psi @08:17:46.59 SS.reconfigure forSampling @08:17:46.62 SS.seek 25.00ml @08:17:59.66 Vacuum too high -- waiting up to 40s @08:17:59.93 Intake @51.0psia, vacuum from 20.0 to 24.0psi @08:17:59.94 SS.reconfigure forSampling @08:17:59.97 SS.seek 25.00ml @08:18:08.58 Stroke completed -- waiting for pressure to equalize ... @08:18:09.22 Intake.select :close @08:18:09.35 SSV.dial :exhaust @08:18:10.15 Exhaust.select :open @08:18:10.25 SS.reconfigure SSexhale @08:18:10.29 SS.seek 0.00ml @08:18:19.07 Exhaust.select :close @08:18:19.16 SSV.dial CBV @08:18:19.18
CBV.dial SSV @08:18:19.96 Allowing 185s for 25.0ml of remaining 875.0ml @08:18:19.97 Intake.select :open @08:18:22.10 Intake @51.2psia, vacuum from 20.0 to 24.0psi @08:18:22.11 SS.reconfigure forSampling @08:18:22.15 SS.seek 25.00ml @08:18:51.69 Vacuum too high -- waiting up to 40s @08:18:51.96 Intake @50.5psia, vacuum from 20.0 to 24.0psi @08:18:51.97 SS.reconfigure forSampling @08:18:52.01 SS.seek 25.00ml @08:19:47.76 Stroke completed -- waiting for pressure to equalize ... @08:19:48.55 Intake.select :close @08:19:48.68 SSV.dial :exhaust @08:19:49.48 Exhaust.select :open @08:19:49.58 SS.reconfigure SSexhale @08:19:49.62 SS.seek 0.00ml @08:19:58.83 Exhaust.select :close @08:19:58.92 SSV.dial CBV @08:19:58.94
CBV.dial SSV @08:19:59.72 Allowing 185s for 25.0ml of remaining 850.0ml @08:19:59.73 Intake.select :open @08:20:01.86 Intake @49.5psia, vacuum from 20.0 to 24.0psi @08:20:01.87 SS.reconfigure forSampling @08:20:01.91 SS.seek 25.00ml @08:21:52.29 Stroke completed -- waiting for pressure to equalize ... @08:21:53.51 Intake.select :close @08:21:53.64 SSV.dial :exhaust @08:21:54.45 Exhaust.select :open @08:21:54.54 SS.reconfigure SSexhale @08:21:54.58 SS.seek 0.00ml @08:22:03.37 Exhaust.select :close @08:22:03.46 SSV.dial CBV @08:22:03.48
CBV.dial SSV @08:22:04.26 Allowing 185s for 25.0ml of remaining 825.0ml @08:22:04.27 Intake.select :open @08:22:06.40 Intake @49.1psia, vacuum from 20.0 to 24.0psi @08:22:06.41 SS.reconfigure forSampling @08:22:06.45 SS.seek 25.00ml @08:22:32.77 Vacuum too high -- waiting up to 40s @08:22:33.04 Intake @49.0psia, vacuum from 20.0 to 24.0psi @08:22:33.05 SS.reconfigure forSampling @08:22:33.09 SS.seek 25.00ml @08:22:35.87 Vacuum too high -- waiting up to 40s @08:22:36.13 Intake @48.9psia, vacuum from 20.0 to 24.0psi @08:22:36.14 SS.reconfigure forSampling @08:22:36.17 SS.seek 25.00ml @08:22:37.87 Vacuum too high -- waiting up to 40s @08:22:38.13 Intake @48.6psia, vacuum from 20.0 to 24.0psi @08:22:38.14 SS.reconfigure forSampling @08:22:38.17 SS.seek 25.00ml @08:22:41.00 Vacuum too high -- waiting up to 40s @08:22:41.26 Intake @48.9psia, vacuum from 20.0 to 24.0psi @08:22:41.27 SS.reconfigure forSampling @08:22:41.31 SS.seek 25.00ml @08:22:43.68 Vacuum too high -- waiting up to 40s @08:22:43.94 Intake @49.3psia, vacuum from 20.0 to 24.0psi @08:22:43.95 SS.reconfigure forSampling @08:22:43.98 SS.seek 25.00ml @08:22:45.68 Vacuum too high -- waiting up to 40s @08:22:45.94 Intake @49.5psia, vacuum from 20.0 to 24.0psi @08:22:45.95 SS.reconfigure forSampling @08:22:45.98 SS.seek 25.00ml @08:22:47.53 Vacuum too high -- waiting up to 40s @08:22:47.79 Intake @49.4psia, vacuum from 20.0 to 24.0psi @08:22:47.80 SS.reconfigure forSampling @08:22:47.83 SS.seek 25.00ml @08:22:49.28 Vacuum too high -- waiting up to 40s @08:22:49.53 Intake @49.4psia, vacuum from 20.0 to 24.0psi @08:22:49.54 SS.reconfigure forSampling @08:22:49.58 SS.seek 25.00ml @08:22:51.25 Vacuum too high -- waiting up to 40s @08:22:51.51 Intake @49.6psia, vacuum from 20.0 to 24.0psi @08:22:51.52 SS.reconfigure forSampling @08:22:51.55 SS.seek 25.00ml @08:22:53.37 Vacuum too high -- waiting up to 40s @08:22:53.63 Intake @49.8psia, vacuum from 20.0 to 24.0psi @08:22:53.64 SS.reconfigure forSampling @08:22:53.67 SS.seek 25.00ml @08:22:54.87 Vacuum too high -- waiting up to 40s @08:22:55.13 Intake @49.4psia, vacuum from 20.0 to 24.0psi @08:22:55.14 SS.reconfigure forSampling @08:22:55.17 SS.seek 25.00ml @08:22:58.99 Vacuum too high -- waiting up to 40s @08:22:59.25 Intake @49.9psia, vacuum from 20.0 to 24.0psi @08:22:59.26 SS.reconfigure forSampling @08:22:59.30 SS.seek 25.00ml @08:23:00.40 Vacuum too high -- waiting up to 40s @08:23:01.35 Intake @49.1psia, vacuum from 20.0 to 24.0psi @08:23:01.36 SS.reconfigure forSampling @08:23:01.39 SS.seek 25.00ml @08:23:03.68 Vacuum too high -- waiting up to 40s @08:23:03.94 Intake @48.9psia, vacuum from 20.0 to 24.0psi @08:23:03.95 SS.reconfigure forSampling @08:23:03.98 SS.seek 25.00ml @08:23:05.49 Vacuum too high -- waiting up to 40s @08:23:05.76 SS.seek 25.00ml @08:23:07.06 Vacuum too high -- waiting up to 40s @08:23:07.32 Intake @48.9psia, vacuum from 20.0 to 24.0psi @08:23:07.33 SS.reconfigure forSampling @08:23:07.37 SS.seek 25.00ml @08:23:08.87 Vacuum too high -- waiting up to 40s @08:23:09.13 Intake @49.1psia, vacuum from 20.0 to 24.0psi @08:23:09.14 SS.reconfigure forSampling @08:23:09.17 SS.seek 25.00ml @08:23:11.87 Vacuum too high -- waiting up to 40s @08:23:12.13 Intake @49.5psia, vacuum from 20.0 to 24.0psi @08:23:12.14 SS.reconfigure forSampling @08:23:12.17 SS.seek 25.00ml @08:23:13.31 Vacuum too high -- waiting up to 40s @08:23:13.57 Intake @49.0psia, vacuum from 20.0 to 24.0psi @08:23:13.58 SS.reconfigure forSampling @08:23:13.61 SS.seek 25.00ml @08:23:14.93 Vacuum too high -- waiting up to 40s @08:23:15.19 Intake @48.5psia, vacuum from 20.0 to 24.0psi @08:23:15.20 SS.reconfigure forSampling @08:23:15.23 SS.seek 25.00ml @08:23:16.46 Vacuum too high -- waiting up to 40s @08:23:16.73 Intake @48.2psia, vacuum from 20.0 to 24.0psi @08:23:16.74 SS.reconfigure forSampling @08:23:16.77 SS.seek 25.00ml @08:23:18.18 Vacuum too high -- waiting up to 40s @08:23:18.44 Intake @48.1psia, vacuum from 20.0 to 24.0psi @08:23:18.45 SS.reconfigure forSampling @08:23:18.48 SS.seek 25.00ml @08:23:19.62 Vacuum too high -- waiting up to 40s @08:23:19.88 Intake @48.0psia, vacuum from 20.0 to 24.0psi @08:23:19.89 SS.reconfigure forSampling @08:23:19.93 SS.seek 25.00ml @08:23:20.99 Vacuum too high -- waiting up to 40s @08:23:21.25 Intake @47.9psia, vacuum from 20.0 to 24.0psi @08:23:21.26 SS.reconfigure forSampling @08:23:21.29 SS.seek 25.00ml @08:23:22.43 Vacuum too high -- waiting up to 40s @08:23:22.69 Intake @47.8psia, vacuum from 20.0 to 24.0psi @08:23:22.70 SS.reconfigure forSampling @08:23:22.73 SS.seek 25.00ml @08:23:24.15 Vacuum too high -- waiting up to 40s @08:23:24.42 Intake @49.2psia, vacuum from 20.0 to 24.0psi @08:23:24.43 SS.reconfigure forSampling @08:23:24.46 SS.seek 25.00ml @08:23:25.59 Vacuum too high -- waiting up to 40s @08:23:25.85 Intake @49.5psia, vacuum from 20.0 to 24.0psi @08:23:25.86 SS.reconfigure forSampling @08:23:25.89 SS.seek 25.00ml @08:23:26.78 Vacuum too high -- waiting up to 40s @08:23:27.04 Intake @48.3psia, vacuum from 20.0 to 24.0psi @08:23:27.05 SS.reconfigure forSampling @08:23:27.08 SS.seek 25.00ml @08:23:28.34 Vacuum too high -- waiting up to 40s @08:23:28.61 Intake @47.9psia, vacuum from 20.0 to 24.0psi @08:23:28.62 SS.reconfigure forSampling @08:23:28.65 SS.seek 25.00ml @08:23:30.02 Vacuum too high -- waiting up to 40s @08:23:30.27 Intake @48.2psia, vacuum from 20.0 to 24.0psi @08:23:30.28 SS.reconfigure forSampling @08:23:30.32 SS.seek 25.00ml @08:23:31.21 Vacuum too high -- waiting up to 40s @08:23:31.47 Intake @48.1psia, vacuum from 20.0 to 24.0psi @08:23:31.48 SS.reconfigure forSampling @08:23:31.51 SS.seek 25.00ml @08:23:32.62 Vacuum too high -- waiting up to 40s @08:23:32.88 Intake @48.0psia, vacuum from 20.0 to 24.0psi @08:23:32.89 SS.reconfigure forSampling @08:23:32.92 SS.seek 25.00ml @08:23:34.09 Vacuum too high -- waiting up to 40s @08:23:34.35 Intake @48.2psia, vacuum from 20.0 to 24.0psi @08:23:34.36 SS.reconfigure forSampling @08:23:34.39 SS.seek 25.00ml @08:23:35.40 Vacuum too high -- waiting up to 40s @08:23:35.66 Intake @48.0psia, vacuum from 20.0 to 24.0psi @08:23:35.67 SS.reconfigure forSampling @08:23:35.70 SS.seek 25.00ml @08:23:36.62 Vacuum too high -- waiting up to 40s @08:23:36.88 Intake @47.9psia, vacuum from 20.0 to 24.0psi @08:23:36.89 SS.reconfigure forSampling @08:23:36.92 SS.seek 25.00ml @08:23:38.09 Vacuum too high -- waiting up to 40s @08:23:38.35 Intake @48.5psia, vacuum from 20.0 to 24.0psi @08:23:38.36 SS.reconfigure forSampling @08:23:38.39 SS.seek 25.00ml @08:23:39.52 Vacuum too high -- waiting up to 40s @08:23:40.38 Intake @48.8psia, vacuum from 20.0 to 24.0psi @08:23:40.39 SS.reconfigure forSampling @08:23:40.43 SS.seek 25.00ml @08:23:42.06 Vacuum too high -- waiting up to 40s @08:23:42.32 Intake @49.4psia, vacuum from 20.0 to 24.0psi @08:23:42.33 SS.reconfigure forSampling @08:23:42.36 SS.seek 25.00ml @08:23:43.27 Vacuum too high -- waiting up to 40s @08:23:43.53 Intake @49.2psia, vacuum from 20.0 to 24.0psi @08:23:43.54 SS.reconfigure forSampling @08:23:43.58 SS.seek 25.00ml @08:23:44.65 Vacuum too high -- waiting up to 40s @08:23:44.91 Intake @48.5psia, vacuum from 20.0 to 24.0psi @08:23:44.92 SS.reconfigure forSampling @08:23:44.96 SS.seek 25.00ml @08:23:46.06 Vacuum too high -- waiting up to 40s @08:23:46.32 Intake @48.1psia, vacuum from 20.0 to 24.0psi @08:23:46.33 SS.reconfigure forSampling @08:23:46.37 SS.seek 25.00ml @08:23:47.37 Vacuum too high -- waiting up to 40s @08:23:47.63 Intake @48.2psia, vacuum from 20.0 to 24.0psi @08:23:47.64 SS.reconfigure forSampling @08:23:47.68 SS.seek 25.00ml @08:23:48.52 Vacuum too high -- waiting up to 40s @08:23:48.78 Intake @47.9psia, vacuum from 20.0 to 24.0psi @08:23:48.79 SS.reconfigure forSampling @08:23:48.83 SS.seek 25.00ml @08:23:49.87 Vacuum too high -- waiting up to 40s @08:23:50.14 SS.seek 25.00ml @08:23:51.12 Vacuum too high -- waiting up to 40s @08:23:51.39 SS.seek 25.00ml @08:23:52.27 Vacuum too high -- waiting up to 40s @08:23:52.53 Intake @48.0psia, vacuum from 20.0 to 24.0psi @08:23:52.54 SS.reconfigure forSampling @08:23:52.58 SS.seek 25.00ml @08:23:53.49 Vacuum too high -- waiting up to 40s @08:23:53.75 Intake @48.3psia, vacuum from 20.0 to 24.0psi @08:23:53.76 SS.reconfigure forSampling @08:23:53.80 SS.seek 25.00ml @08:23:54.71 Vacuum too high -- waiting up to 40s @08:23:54.97 Intake @48.6psia, vacuum from 20.0 to 24.0psi @08:23:54.98 SS.reconfigure forSampling @08:23:55.02 SS.seek 25.00ml @08:23:56.12 Vacuum too high -- waiting up to 40s @08:23:56.38 Intake @49.1psia, vacuum from 20.0 to 24.0psi @08:23:56.39 SS.reconfigure forSampling @08:23:56.42 SS.seek 25.00ml @08:23:57.43 Vacuum too high -- waiting up to 40s @08:23:57.69 Intake @49.2psia, vacuum from 20.0 to 24.0psi @08:23:57.70 SS.reconfigure forSampling @08:23:57.73 SS.seek 25.00ml @08:23:58.89 Vacuum too high -- waiting up to 40s @08:23:59.16 Intake @48.9psia, vacuum from 20.0 to 24.0psi @08:23:59.17 SS.reconfigure forSampling @08:23:59.20 SS.seek 25.00ml @08:24:00.37 Vacuum too high -- waiting up to 40s @08:24:00.63 Intake @48.6psia, vacuum from 20.0 to 24.0psi @08:24:00.64 SS.reconfigure forSampling @08:24:00.67 SS.seek 25.00ml @08:24:01.74 Vacuum too high -- waiting up to 40s @08:24:02.00 Intake @48.3psia, vacuum from 20.0 to 24.0psi @08:24:02.01 SS.reconfigure forSampling @08:24:02.04 SS.seek 25.00ml @08:24:02.93 Vacuum too high -- waiting up to 40s @08:24:03.19 Intake @47.9psia, vacuum from 20.0 to 24.0psi @08:24:03.20 SS.reconfigure forSampling @08:24:03.24 SS.seek 25.00ml @08:24:04.15 Vacuum too high -- waiting up to 40s @08:24:04.41 Intake @47.9psia, vacuum from 20.0 to 24.0psi @08:24:04.42 SS.reconfigure forSampling @08:24:04.45 SS.seek 25.00ml @08:24:05.27 Vacuum too high -- waiting up to 40s @08:24:05.53 Intake @47.8psia, vacuum from 20.0 to 24.0psi @08:24:05.54 SS.reconfigure forSampling @08:24:05.57 SS.seek 25.00ml @08:24:06.68 Vacuum too high -- waiting up to 40s @08:24:06.94 Intake @48.3psia, vacuum from 20.0 to 24.0psi @08:24:06.95 SS.reconfigure forSampling @08:24:06.98 SS.seek 25.00ml @08:24:07.96 Vacuum too high -- waiting up to 40s @08:24:08.23 Intake @48.9psia, vacuum from 20.0 to 24.0psi @08:24:08.25 SS.reconfigure forSampling @08:24:08.28 SS.seek 25.00ml @08:24:09.18 Vacuum too high -- waiting up to 40s @08:24:09.44 Intake @49.1psia, vacuum from 20.0 to 24.0psi @08:24:09.45 SS.reconfigure forSampling @08:24:09.48 SS.seek 25.00ml @08:24:10.30 Vacuum too high -- waiting up to 40s @08:24:10.56 Intake @48.4psia, vacuum from 20.0 to 24.0psi @08:24:10.57 SS.reconfigure forSampling @08:24:10.60 SS.seek 25.00ml @08:24:11.59 Vacuum too high -- waiting up to 40s @08:24:11.85 Intake @48.5psia, vacuum from 20.0 to 24.0psi @08:24:11.86 SS.reconfigure forSampling @08:24:11.89 SS.seek 25.00ml @08:24:13.99 Vacuum too high -- waiting up to 40s @08:24:14.25 Intake @47.8psia, vacuum from 20.0 to 24.0psi @08:24:14.26 SS.reconfigure forSampling @08:24:14.29 SS.seek 25.00ml @08:24:15.27 Vacuum too high -- waiting up to 40s @08:24:15.75 Intake @48.9psia, vacuum from 20.0 to 24.0psi @08:24:15.76 SS.reconfigure forSampling @08:24:15.80 SS.seek 25.00ml @08:24:16.99 Vacuum too high -- waiting up to 40s @08:24:17.25 Intake @49.0psia, vacuum from 20.0 to 24.0psi @08:24:17.26 SS.reconfigure forSampling @08:24:17.30 SS.seek 25.00ml @08:24:18.30 Vacuum too high -- waiting up to 40s @08:24:18.56 Intake @49.3psia, vacuum from 20.0 to 24.0psi @08:24:18.57 SS.reconfigure forSampling @08:24:18.61 SS.seek 25.00ml @08:24:19.55 Vacuum too high -- waiting up to 40s @08:24:19.81 Intake @49.4psia, vacuum from 20.0 to 24.0psi @08:24:19.82 SS.reconfigure forSampling @08:24:19.86 SS.seek 25.00ml @08:24:20.71 Vacuum too high -- waiting up to 40s @08:24:20.97 Intake @49.6psia, vacuum from 20.0 to 24.0psi @08:24:20.98 SS.reconfigure forSampling @08:24:21.02 SS.seek 25.00ml @08:24:21.87 Vacuum too high -- waiting up to 40s @08:24:22.13 Intake @49.8psia, vacuum from 20.0 to 24.0psi @08:24:22.14 SS.reconfigure forSampling @08:24:22.18 SS.seek 25.00ml @08:24:23.02 Vacuum too high -- waiting up to 40s @08:24:23.28 Intake @50.1psia, vacuum from 20.0 to 24.0psi @08:24:23.29 SS.reconfigure forSampling @08:24:23.32 SS.seek 25.00ml @08:24:24.15 Vacuum too high -- waiting up to 40s @08:24:24.41 Intake @49.9psia, vacuum from 20.0 to 24.0psi @08:24:24.42 SS.reconfigure forSampling @08:24:24.45 SS.seek 25.00ml @08:24:25.37 Vacuum too high -- waiting up to 40s @08:24:25.63 Intake @49.5psia, vacuum from 20.0 to 24.0psi @08:24:25.64 SS.reconfigure forSampling @08:24:25.67 SS.seek 25.00ml @08:24:26.52 Vacuum too high -- waiting up to 40s @08:24:26.78 Intake @49.1psia, vacuum from 20.0 to 24.0psi @08:24:26.79 SS.reconfigure forSampling @08:24:26.83 SS.seek 25.00ml @08:24:27.58 Vacuum too high -- waiting up to 40s @08:24:27.84 Intake @48.7psia, vacuum from 20.0 to 24.0psi @08:24:27.85 SS.reconfigure forSampling @08:24:27.88 SS.seek 25.00ml @08:24:28.65 Vacuum too high -- waiting up to 40s @08:24:28.91 Intake @48.6psia, vacuum from 20.0 to 24.0psi @08:24:28.92 SS.reconfigure forSampling @08:24:28.95 SS.seek 25.00ml @08:24:29.96 Vacuum too high -- waiting up to 40s @08:24:30.22 Intake @48.6psia, vacuum from 20.0 to 24.0psi @08:24:30.23 SS.reconfigure forSampling @08:24:30.26 SS.seek 25.00ml @08:24:31.12 Vacuum too high -- waiting up to 40s @08:24:31.38 Intake @48.3psia, vacuum from 20.0 to 24.0psi @08:24:31.39 SS.reconfigure forSampling @08:24:31.42 SS.seek 25.00ml @08:24:32.33 Vacuum too high -- waiting up to 40s @08:24:32.81 Intake @48.7psia, vacuum from 20.0 to 24.0psi @08:24:32.82 SS.reconfigure forSampling @08:24:32.85 SS.seek 25.00ml @08:24:33.53 Stroke completed -- waiting for pressure to equalize ... @08:24:35.34 Intake.select :close @08:24:35.47 SSV.dial :exhaust @08:24:36.27 Exhaust.select :open @08:24:36.36 SS.reconfigure SSexhale @08:24:36.40 SS.seek 0.00ml @08:24:45.53 Exhaust.select :close @08:24:45.62 SSV.dial CBV @08:24:45.64
CBV.dial SSV @08:24:46.44 Allowing 185s for 25.0ml of remaining 800.0ml @08:24:46.45 Intake.select :open @08:24:48.58 Intake @48.9psia, vacuum from 20.0 to 24.0psi @08:24:48.59 SS.reconfigure forSampling @08:24:48.63 SS.seek 25.00ml @08:24:50.30 Vacuum too high -- waiting up to 40s @08:24:50.55 Intake @48.7psia, vacuum from 20.0 to 24.0psi @08:24:50.56 SS.reconfigure forSampling @08:24:50.60 SS.seek 25.00ml @08:24:51.52 Vacuum too high -- waiting up to 40s @08:24:51.78 Intake @48.8psia, vacuum from 20.0 to 24.0psi @08:24:51.79 SS.reconfigure forSampling @08:24:51.82 SS.seek 25.00ml @08:24:52.86 Vacuum too high -- waiting up to 40s @08:24:53.12 Intake @48.7psia, vacuum from 20.0 to 24.0psi @08:24:53.13 SS.reconfigure forSampling @08:24:53.17 SS.seek 25.00ml @08:24:54.24 Vacuum too high -- waiting up to 40s @08:24:54.50 Intake @49.2psia, vacuum from 20.0 to 24.0psi @08:24:54.51 SS.reconfigure forSampling @08:24:54.54 SS.seek 25.00ml @08:24:55.43 Vacuum too high -- waiting up to 40s @08:24:56.30 Intake @49.6psia, vacuum from 20.0 to 24.0psi @08:24:56.31 SS.reconfigure forSampling @08:24:56.34 SS.seek 25.00ml @08:24:57.71 Vacuum too high -- waiting up to 40s @08:24:57.99 Intake @49.3psia, vacuum from 20.0 to 24.0psi @08:24:58.00 SS.reconfigure forSampling @08:24:58.03 SS.seek 25.00ml @08:24:58.93 Vacuum too high -- waiting up to 40s @08:24:59.19 Intake @49.0psia, vacuum from 20.0 to 24.0psi @08:24:59.20 SS.reconfigure forSampling @08:24:59.24 SS.seek 25.00ml @08:25:00.24 Vacuum too high -- waiting up to 40s @08:25:00.50 Intake @48.7psia, vacuum from 20.0 to 24.0psi @08:25:00.51 SS.reconfigure forSampling @08:25:00.55 SS.seek 25.00ml @08:25:01.43 Vacuum too high -- waiting up to 40s @08:25:01.69 Intake @48.8psia, vacuum from 20.0 to 24.0psi @08:25:01.70 SS.reconfigure forSampling @08:25:01.74 SS.seek 25.00ml @08:25:02.58 Vacuum too high -- waiting up to 40s @08:25:02.84 Intake @48.9psia, vacuum from 20.0 to 24.0psi @08:25:02.85 SS.reconfigure forSampling @08:25:02.88 SS.seek 25.00ml @08:25:03.71 Vacuum too high -- waiting up to 40s @08:25:03.97 Intake @48.7psia, vacuum from 20.0 to 24.0psi @08:25:03.98 SS.reconfigure forSampling @08:25:04.01 SS.seek 25.00ml @08:25:04.80 Vacuum too high -- waiting up to 40s @08:25:05.06 Intake @48.6psia, vacuum from 20.0 to 24.0psi @08:25:05.07 SS.reconfigure forSampling @08:25:05.10 SS.seek 25.00ml @08:25:05.99 Vacuum too high -- waiting up to 40s @08:25:06.25 Intake @48.8psia, vacuum from 20.0 to 24.0psi @08:25:06.26 SS.reconfigure forSampling @08:25:06.29 SS.seek 25.00ml @08:25:07.08 Vacuum too high -- waiting up to 40s @08:25:07.34 Intake @49.4psia, vacuum from 20.0 to 24.0psi @08:25:07.35 SS.reconfigure forSampling @08:25:07.38 SS.seek 25.00ml @08:25:08.18 Vacuum too high -- waiting up to 40s @08:25:08.44 Intake @49.6psia, vacuum from 20.0 to 24.0psi @08:25:08.45 SS.reconfigure forSampling @08:25:08.49 SS.seek 25.00ml @08:25:09.39 Vacuum too high -- waiting up to 40s @08:25:09.65 Intake @49.3psia, vacuum from 20.0 to 24.0psi @08:25:09.66 SS.reconfigure forSampling @08:25:09.69 SS.seek 25.00ml @08:25:10.52 Vacuum too high -- waiting up to 40s @08:25:10.78 Intake @49.1psia, vacuum from 20.0 to 24.0psi @08:25:10.79 SS.reconfigure forSampling @08:25:10.82 SS.seek 25.00ml @08:25:11.64 Vacuum too high -- waiting up to 40s @08:25:11.90 Intake @48.9psia, vacuum from 20.0 to 24.0psi @08:25:11.91 SS.reconfigure forSampling @08:25:11.94 SS.seek 25.00ml @08:25:12.86 Vacuum too high -- waiting up to 40s @08:25:13.12 Intake @48.6psia, vacuum from 20.0 to 24.0psi @08:25:13.13 SS.reconfigure forSampling @08:25:13.16 SS.seek 25.00ml @08:25:14.02 Vacuum too high -- waiting up to 40s @08:25:14.28 Intake @48.8psia, vacuum from 20.0 to 24.0psi @08:25:14.29 SS.reconfigure forSampling @08:25:14.32 SS.seek 25.00ml @08:25:15.11 Vacuum too high -- waiting up to 40s @08:25:15.37 Intake @48.9psia, vacuum from 20.0 to 24.0psi @08:25:15.38 SS.reconfigure forSampling @08:25:15.41 SS.seek 25.00ml @08:25:16.30 Vacuum too high -- waiting up to 40s @08:25:16.56 Intake @48.8psia, vacuum from 20.0 to 24.0psi @08:25:16.57 SS.reconfigure forSampling @08:25:16.60 SS.seek 25.00ml @08:25:17.46 Vacuum too high -- waiting up to 40s @08:25:17.73 Intake @48.9psia, vacuum from 20.0 to 24.0psi @08:25:17.74 SS.reconfigure forSampling @08:25:17.78 SS.seek 25.00ml @08:25:18.72 Vacuum too high -- waiting up to 40s @08:25:18.98 Intake @49.5psia, vacuum from 20.0 to 24.0psi @08:25:18.99 SS.reconfigure forSampling @08:25:19.03 SS.seek 25.00ml @08:25:19.84 Vacuum too high -- waiting up to 40s @08:25:20.10 Intake @49.3psia, vacuum from 20.0 to 24.0psi @08:25:20.11 SS.reconfigure forSampling @08:25:20.14 SS.seek 25.00ml @08:25:20.84 Vacuum too high -- waiting up to 40s @08:25:21.10 Intake @48.7psia, vacuum from 20.0 to 24.0psi @08:25:21.11 SS.reconfigure forSampling @08:25:21.14 SS.seek 25.00ml @08:25:21.94 Vacuum too high -- waiting up to 40s @08:25:22.20 Intake @48.4psia, vacuum from 20.0 to 24.0psi @08:25:22.21 SS.reconfigure forSampling @08:25:22.24 SS.seek 25.00ml @08:25:23.03 Vacuum too high -- waiting up to 40s @08:25:23.29 Intake @48.6psia, vacuum from 20.0 to 24.0psi @08:25:23.30 SS.reconfigure forSampling @08:25:23.33 SS.seek 25.00ml @08:25:24.12 Vacuum too high -- waiting up to 40s @08:25:24.99 Intake @48.7psia, vacuum from 20.0 to 24.0psi @08:25:25.00 SS.reconfigure forSampling @08:25:25.04 SS.seek 25.00ml @08:25:26.40 Vacuum too high -- waiting up to 40s @08:25:26.66 Intake @48.4psia, vacuum from 20.0 to 24.0psi @08:25:26.67 SS.reconfigure forSampling @08:25:26.71 SS.seek 25.00ml @08:25:27.56 Vacuum too high -- waiting up to 40s @08:25:27.82 Intake @48.5psia, vacuum from 20.0 to 24.0psi @08:25:27.84 SS.reconfigure forSampling @08:25:27.87 SS.seek 25.00ml @08:25:28.65 Vacuum too high -- waiting up to 40s @08:25:28.91 Intake @48.8psia, vacuum from 20.0 to 24.0psi @08:25:28.92 SS.reconfigure forSampling @08:25:28.96 SS.seek 25.00ml @08:25:29.78 Vacuum too high -- waiting up to 40s @08:25:30.04 Intake @48.8psia, vacuum from 20.0 to 24.0psi @08:25:30.05 SS.reconfigure forSampling @08:25:30.09 SS.seek 25.00ml @08:25:30.87 Vacuum too high -- waiting up to 40s @08:25:31.13 Intake @48.6psia, vacuum from 20.0 to 24.0psi @08:25:31.14 SS.reconfigure forSampling @08:25:31.17 SS.seek 25.00ml @08:25:31.90 Vacuum too high -- waiting up to 40s @08:25:32.16 Intake @48.1psia, vacuum from 20.0 to 24.0psi @08:25:32.17 SS.reconfigure forSampling @08:25:32.21 SS.seek 25.00ml @08:25:32.93 Vacuum too high -- waiting up to 40s @08:25:33.20 Intake @47.7psia, vacuum from 20.0 to 24.0psi @08:25:33.21 SS.reconfigure forSampling @08:25:33.25 SS.seek 25.00ml @08:25:34.09 Vacuum too high -- waiting up to 40s @08:25:34.36 SS.seek 25.00ml @08:25:35.18 Vacuum too high -- waiting up to 40s @08:25:35.44 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:25:35.45 SS.reconfigure forSampling @08:25:35.48 SS.seek 25.00ml @08:25:36.18 Vacuum too high -- waiting up to 40s @08:25:36.45 SS.seek 25.00ml @08:25:37.12 Vacuum too high -- waiting up to 40s @08:25:37.38 Intake @47.7psia, vacuum from 20.0 to 24.0psi @08:25:37.39 SS.reconfigure forSampling @08:25:37.42 SS.seek 25.00ml @08:25:38.18 Vacuum too high -- waiting up to 40s @08:25:38.44 Intake @48.0psia, vacuum from 20.0 to 24.0psi @08:25:38.45 SS.reconfigure forSampling @08:25:38.48 SS.seek 25.00ml @08:25:39.15 Vacuum too high -- waiting up to 40s @08:25:39.41 Intake @48.5psia, vacuum from 20.0 to 24.0psi @08:25:39.42 SS.reconfigure forSampling @08:25:39.46 SS.seek 25.00ml @08:25:40.06 Vacuum too high -- waiting up to 40s @08:25:40.32 Intake @48.6psia, vacuum from 20.0 to 24.0psi @08:25:40.33 SS.reconfigure forSampling @08:25:40.36 SS.seek 25.00ml @08:25:41.06 Vacuum too high -- waiting up to 40s @08:25:41.32 Intake @48.2psia, vacuum from 20.0 to 24.0psi @08:25:41.33 SS.reconfigure forSampling @08:25:41.36 SS.seek 25.00ml @08:25:42.12 Vacuum too high -- waiting up to 40s @08:25:42.38 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:25:42.39 SS.reconfigure forSampling @08:25:42.43 SS.seek 25.00ml @08:25:43.18 Vacuum too high -- waiting up to 40s @08:25:43.44 Intake @47.0psia, vacuum from 20.0 to 24.0psi @08:25:43.45 SS.reconfigure forSampling @08:25:43.49 SS.seek 25.00ml @08:25:44.22 Vacuum too high -- waiting up to 40s @08:25:44.48 Intake @47.4psia, vacuum from 20.0 to 24.0psi @08:25:44.49 SS.reconfigure forSampling @08:25:44.52 SS.seek 25.00ml @08:25:45.22 Vacuum too high -- waiting up to 40s @08:25:45.48 Intake @47.5psia, vacuum from 20.0 to 24.0psi @08:25:45.49 SS.reconfigure forSampling @08:25:45.53 SS.seek 25.00ml @08:25:46.18 Vacuum too high -- waiting up to 40s @08:25:46.44 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:25:46.45 SS.reconfigure forSampling @08:25:46.49 SS.seek 25.00ml @08:25:47.15 Vacuum too high -- waiting up to 40s @08:25:47.41 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:25:47.42 SS.reconfigure forSampling @08:25:47.45 SS.seek 25.00ml @08:25:48.15 Vacuum too high -- waiting up to 40s @08:25:49.00 Intake @47.9psia, vacuum from 20.0 to 24.0psi @08:25:49.05 SS.reconfigure forSampling @08:25:49.08 SS.seek 25.00ml @08:25:50.34 Vacuum too high -- waiting up to 40s @08:25:50.60 Intake @48.0psia, vacuum from 20.0 to 24.0psi @08:25:50.61 SS.reconfigure forSampling @08:25:50.64 SS.seek 25.00ml @08:25:51.34 Vacuum too high -- waiting up to 40s @08:25:51.60 Intake @47.8psia, vacuum from 20.0 to 24.0psi @08:25:51.61 SS.reconfigure forSampling @08:25:51.64 SS.seek 25.00ml @08:25:53.43 Vacuum too high -- waiting up to 40s @08:25:53.70 Intake @46.9psia, vacuum from 20.0 to 24.0psi @08:25:53.71 SS.reconfigure forSampling @08:25:53.74 SS.seek 25.00ml @08:25:54.53 Vacuum too high -- waiting up to 40s @08:25:54.79 Intake @47.0psia, vacuum from 20.0 to 24.0psi @08:25:54.80 SS.reconfigure forSampling @08:25:54.84 SS.seek 25.00ml @08:25:55.53 Vacuum too high -- waiting up to 40s @08:25:55.79 Intake @46.3psia, vacuum from 20.0 to 24.0psi @08:25:55.80 SS.reconfigure forSampling @08:25:55.84 SS.seek 25.00ml @08:25:56.59 Vacuum too high -- waiting up to 40s @08:25:56.85 Intake @46.2psia, vacuum from 20.0 to 24.0psi @08:25:56.86 SS.reconfigure forSampling @08:25:56.90 SS.seek 25.00ml @08:25:57.62 Vacuum too high -- waiting up to 40s @08:25:58.10 Intake @46.3psia, vacuum from 20.0 to 24.0psi @08:25:58.11 SS.reconfigure forSampling @08:25:58.15 SS.seek 25.00ml @08:25:59.21 Vacuum too high -- waiting up to 40s @08:25:59.69 Intake @47.2psia, vacuum from 20.0 to 24.0psi @08:25:59.70 SS.reconfigure forSampling @08:25:59.74 SS.seek 25.00ml @08:26:00.53 Vacuum too high -- waiting up to 40s @08:26:00.79 Intake @47.3psia, vacuum from 20.0 to 24.0psi @08:26:00.80 SS.reconfigure forSampling @08:26:00.83 SS.seek 25.00ml @08:26:01.53 Vacuum too high -- waiting up to 40s @08:26:01.79 Intake @46.4psia, vacuum from 20.0 to 24.0psi @08:26:01.80 SS.reconfigure forSampling @08:26:01.83 SS.seek 25.00ml @08:26:02.56 Vacuum too high -- waiting up to 40s @08:26:02.82 Intake @45.9psia, vacuum from 20.0 to 24.0psi @08:26:02.83 SS.reconfigure forSampling @08:26:02.86 SS.seek 25.00ml @08:26:03.53 Vacuum too high -- waiting up to 40s @08:26:03.79 Intake @45.9psia, vacuum from 20.0 to 24.0psi @08:26:03.80 SS.reconfigure forSampling @08:26:03.83 SS.seek 25.00ml @08:26:04.46 Vacuum too high -- waiting up to 40s @08:26:04.72 Intake @45.8psia, vacuum from 20.0 to 24.0psi @08:26:04.73 SS.reconfigure forSampling @08:26:04.76 SS.seek 25.00ml @08:26:05.40 Vacuum too high -- waiting up to 40s @08:26:05.66 Intake @46.2psia, vacuum from 20.0 to 24.0psi @08:26:05.67 SS.reconfigure forSampling @08:26:05.71 SS.seek 25.00ml @08:26:06.37 Vacuum too high -- waiting up to 40s @08:26:06.84 Intake @46.1psia, vacuum from 20.0 to 24.0psi @08:26:06.85 SS.reconfigure forSampling @08:26:06.89 SS.seek 25.00ml @08:26:07.75 Vacuum too high -- waiting up to 40s @08:26:08.01 Intake @46.9psia, vacuum from 20.0 to 24.0psi @08:26:08.02 SS.reconfigure forSampling @08:26:08.05 SS.seek 25.00ml @08:26:08.71 Vacuum too high -- waiting up to 40s @08:26:09.19 Intake @47.4psia, vacuum from 20.0 to 24.0psi @08:26:09.20 SS.reconfigure forSampling @08:26:09.24 SS.seek 25.00ml @08:26:10.09 Vacuum too high -- waiting up to 40s @08:26:10.35 Intake @47.2psia, vacuum from 20.0 to 24.0psi @08:26:10.36 SS.reconfigure forSampling @08:26:10.39 SS.seek 25.00ml @08:26:11.03 Vacuum too high -- waiting up to 40s @08:26:11.51 Intake @47.4psia, vacuum from 20.0 to 24.0psi @08:26:11.52 SS.reconfigure forSampling @08:26:11.55 SS.seek 25.00ml @08:26:12.27 Vacuum too high -- waiting up to 40s @08:26:12.54 Intake @47.3psia, vacuum from 20.0 to 24.0psi @08:26:12.55 SS.reconfigure forSampling @08:26:12.59 SS.seek 25.00ml @08:26:13.28 Vacuum too high -- waiting up to 40s @08:26:13.54 Intake @47.2psia, vacuum from 20.0 to 24.0psi @08:26:13.55 SS.reconfigure forSampling @08:26:13.58 SS.seek 25.00ml @08:26:14.31 Vacuum too high -- waiting up to 40s @08:26:14.57 Intake @47.3psia, vacuum from 20.0 to 24.0psi @08:26:14.58 SS.reconfigure forSampling @08:26:14.61 SS.seek 25.00ml @08:26:15.25 Vacuum too high -- waiting up to 40s @08:26:15.51 Intake @47.2psia, vacuum from 20.0 to 24.0psi @08:26:15.52 SS.reconfigure forSampling @08:26:15.55 SS.seek 25.00ml @08:26:16.21 Vacuum too high -- waiting up to 40s @08:26:16.47 Intake @47.2psia, vacuum from 20.0 to 24.0psi @08:26:16.48 SS.reconfigure forSampling @08:26:16.52 SS.seek 25.00ml @08:26:17.18 Vacuum too high -- waiting up to 40s @08:26:17.44 Intake @47.4psia, vacuum from 20.0 to 24.0psi @08:26:18.09 SS.reconfigure forSampling @08:26:18.12 SS.seek 25.00ml @08:26:19.46 Vacuum too high -- waiting up to 40s @08:26:19.72 Intake @47.9psia, vacuum from 20.0 to 24.0psi @08:26:19.73 SS.reconfigure forSampling @08:26:19.77 SS.seek 25.00ml @08:26:20.43 Vacuum too high -- waiting up to 40s @08:26:20.70 SS.seek 25.00ml @08:26:21.31 Vacuum too high -- waiting up to 40s @08:26:21.57 Intake @47.7psia, vacuum from 20.0 to 24.0psi @08:26:21.58 SS.reconfigure forSampling @08:26:21.62 SS.seek 25.00ml @08:26:22.31 Vacuum too high -- waiting up to 40s @08:26:22.57 Intake @47.3psia, vacuum from 20.0 to 24.0psi @08:26:22.58 SS.reconfigure forSampling @08:26:22.62 SS.seek 25.00ml @08:26:23.34 Vacuum too high -- waiting up to 40s @08:26:23.60 Intake @47.1psia, vacuum from 20.0 to 24.0psi @08:26:23.61 SS.reconfigure forSampling @08:26:23.65 SS.seek 25.00ml @08:26:24.34 Vacuum too high -- waiting up to 40s @08:26:24.60 Intake @47.3psia, vacuum from 20.0 to 24.0psi @08:26:24.61 SS.reconfigure forSampling @08:26:24.65 SS.seek 25.00ml @08:26:25.24 Vacuum too high -- waiting up to 40s @08:26:25.50 Intake @47.5psia, vacuum from 20.0 to 24.0psi @08:26:25.51 SS.reconfigure forSampling @08:26:25.55 SS.seek 25.00ml @08:26:26.18 Vacuum too high -- waiting up to 40s @08:26:26.44 Intake @47.7psia, vacuum from 20.0 to 24.0psi @08:26:26.45 SS.reconfigure forSampling @08:26:26.49 SS.seek 25.00ml @08:26:27.09 Vacuum too high -- waiting up to 40s @08:26:27.35 Intake @47.7psia, vacuum from 20.0 to 24.0psi @08:26:27.36 SS.reconfigure forSampling @08:26:27.40 SS.seek 25.00ml @08:26:28.06 Vacuum too high -- waiting up to 40s @08:26:28.32 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:26:28.33 SS.reconfigure forSampling @08:26:28.37 SS.seek 25.00ml @08:26:29.03 Vacuum too high -- waiting up to 40s @08:26:29.50 Intake @47.2psia, vacuum from 20.0 to 24.0psi @08:26:29.51 SS.reconfigure forSampling @08:26:29.55 SS.seek 25.00ml @08:26:30.43 Vacuum too high -- waiting up to 40s @08:26:30.69 Intake @47.1psia, vacuum from 20.0 to 24.0psi @08:26:30.70 SS.reconfigure forSampling @08:26:30.73 SS.seek 25.00ml @08:26:31.37 Vacuum too high -- waiting up to 40s @08:26:31.63 Intake @47.2psia, vacuum from 20.0 to 24.0psi @08:26:31.64 SS.reconfigure forSampling @08:26:31.67 SS.seek 25.00ml @08:26:32.34 Vacuum too high -- waiting up to 40s @08:26:32.60 Intake @47.2psia, vacuum from 20.0 to 24.0psi @08:26:32.61 SS.reconfigure forSampling @08:26:32.64 SS.seek 25.00ml @08:26:33.24 Vacuum too high -- waiting up to 40s @08:26:33.51 SS.seek 25.00ml @08:26:34.09 Vacuum too high -- waiting up to 40s @08:26:34.58 SS.seek 25.00ml @08:26:35.46 Vacuum too high -- waiting up to 40s @08:26:35.72 Intake @47.3psia, vacuum from 20.0 to 24.0psi @08:26:35.73 SS.reconfigure forSampling @08:26:35.77 SS.seek 25.00ml @08:26:36.46 Vacuum too high -- waiting up to 40s @08:26:36.93 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:26:36.94 SS.reconfigure forSampling @08:26:36.97 SS.seek 25.00ml @08:26:37.71 Vacuum too high -- waiting up to 40s @08:26:37.97 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:26:37.98 SS.reconfigure forSampling @08:26:38.02 SS.seek 25.00ml @08:26:38.62 Vacuum too high -- waiting up to 40s @08:26:38.88 Intake @47.8psia, vacuum from 20.0 to 24.0psi @08:26:38.89 SS.reconfigure forSampling @08:26:38.93 SS.seek 25.00ml @08:26:39.56 Vacuum too high -- waiting up to 40s @08:26:39.83 SS.seek 25.00ml @08:26:40.40 Vacuum too high -- waiting up to 40s @08:26:40.66 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:26:40.67 SS.reconfigure forSampling @08:26:40.71 SS.seek 25.00ml @08:26:41.37 Vacuum too high -- waiting up to 40s @08:26:41.84 Intake @47.9psia, vacuum from 20.0 to 24.0psi @08:26:41.85 SS.reconfigure forSampling @08:26:41.89 SS.seek 25.00ml @08:26:42.71 Vacuum too high -- waiting up to 40s @08:26:43.61 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:26:43.62 SS.reconfigure forSampling @08:26:43.66 SS.seek 25.00ml @08:26:44.74 Vacuum too high -- waiting up to 40s @08:26:45.01 Intake @47.1psia, vacuum from 20.0 to 24.0psi @08:26:45.02 SS.reconfigure forSampling @08:26:45.05 SS.seek 25.00ml @08:26:45.71 Vacuum too high -- waiting up to 40s @08:26:46.19 Intake @47.1psia, vacuum from 20.0 to 24.0psi @08:26:46.20 SS.reconfigure forSampling @08:26:46.24 SS.seek 25.00ml @08:26:47.09 Vacuum too high -- waiting up to 40s @08:26:47.57 SS.seek 25.00ml @08:26:48.34 Vacuum too high -- waiting up to 40s @08:26:48.82 Intake @47.1psia, vacuum from 20.0 to 24.0psi @08:26:48.83 SS.reconfigure forSampling @08:26:48.87 SS.seek 25.00ml @08:26:49.81 Vacuum too high -- waiting up to 40s @08:26:50.29 Intake @47.2psia, vacuum from 20.0 to 24.0psi @08:26:50.30 SS.reconfigure forSampling @08:26:50.34 SS.seek 25.00ml @08:26:51.24 Vacuum too high -- waiting up to 40s @08:26:51.50 Intake @47.1psia, vacuum from 20.0 to 24.0psi @08:26:51.51 SS.reconfigure forSampling @08:26:51.55 SS.seek 25.00ml @08:26:52.18 Vacuum too high -- waiting up to 40s @08:26:52.66 Intake @47.4psia, vacuum from 20.0 to 24.0psi @08:26:52.67 SS.reconfigure forSampling @08:26:52.71 SS.seek 25.00ml @08:26:53.56 Vacuum too high -- waiting up to 40s @08:26:54.04 Intake @48.0psia, vacuum from 20.0 to 24.0psi @08:26:54.05 SS.reconfigure forSampling @08:26:54.08 SS.seek 25.00ml @08:26:54.84 Vacuum too high -- waiting up to 40s @08:26:55.10 Intake @47.5psia, vacuum from 20.0 to 24.0psi @08:26:55.11 SS.reconfigure forSampling @08:26:55.14 SS.seek 25.00ml @08:26:55.74 Vacuum too high -- waiting up to 40s @08:26:56.00 Intake @46.6psia, vacuum from 20.0 to 24.0psi @08:26:56.01 SS.reconfigure forSampling @08:26:56.05 SS.seek 25.00ml @08:26:56.68 Vacuum too high -- waiting up to 40s @08:26:56.94 Intake @46.6psia, vacuum from 20.0 to 24.0psi @08:26:56.95 SS.reconfigure forSampling @08:26:56.99 SS.seek 25.00ml @08:26:57.62 Vacuum too high -- waiting up to 40s @08:26:58.12 SS.seek 25.00ml @08:26:58.84 Vacuum too high -- waiting up to 40s @08:26:59.32 Intake @46.7psia, vacuum from 20.0 to 24.0psi @08:26:59.33 SS.reconfigure forSampling @08:26:59.37 SS.seek 25.00ml @08:27:00.09 Vacuum too high -- waiting up to 40s @08:27:00.58 Intake @46.5psia, vacuum from 20.0 to 24.0psi @08:27:00.59 SS.reconfigure forSampling @08:27:00.62 SS.seek 25.00ml @08:27:01.43 Vacuum too high -- waiting up to 40s @08:27:01.90 Intake @46.6psia, vacuum from 20.0 to 24.0psi @08:27:01.91 SS.reconfigure forSampling @08:27:01.94 SS.seek 25.00ml @08:27:02.62 Vacuum too high -- waiting up to 40s @08:27:03.09 Intake @46.7psia, vacuum from 20.0 to 24.0psi @08:27:03.10 SS.reconfigure forSampling @08:27:03.13 SS.seek 25.00ml @08:27:03.93 Vacuum too high -- waiting up to 40s @08:27:04.41 Intake @47.3psia, vacuum from 20.0 to 24.0psi @08:27:04.42 SS.reconfigure forSampling @08:27:04.45 SS.seek 25.00ml @08:27:05.37 Vacuum too high -- waiting up to 40s @08:27:05.84 Intake @47.5psia, vacuum from 20.0 to 24.0psi @08:27:05.85 SS.reconfigure forSampling @08:27:05.88 SS.seek 25.00ml @08:27:06.68 Vacuum too high -- waiting up to 40s @08:27:06.94 Intake @47.2psia, vacuum from 20.0 to 24.0psi @08:27:06.95 SS.reconfigure forSampling @08:27:06.98 SS.seek 25.00ml @08:27:07.56 Vacuum too high -- waiting up to 40s @08:27:07.82 Intake @46.7psia, vacuum from 20.0 to 24.0psi @08:27:07.83 SS.reconfigure forSampling @08:27:07.86 SS.seek 25.00ml @08:27:08.46 Vacuum too high -- waiting up to 40s @08:27:09.56 Intake @46.6psia, vacuum from 20.0 to 24.0psi @08:27:09.57 SS.reconfigure forSampling @08:27:09.60 SS.seek 25.00ml @08:27:10.87 Vacuum too high -- waiting up to 40s @08:27:11.35 Intake @46.8psia, vacuum from 20.0 to 24.0psi @08:27:11.36 SS.reconfigure forSampling @08:27:11.40 SS.seek 25.00ml @08:27:12.30 Vacuum too high -- waiting up to 40s @08:27:12.56 Intake @46.5psia, vacuum from 20.0 to 24.0psi @08:27:12.57 SS.reconfigure forSampling @08:27:12.61 SS.seek 25.00ml @08:27:13.27 Vacuum too high -- waiting up to 40s @08:27:13.75 Intake @46.6psia, vacuum from 20.0 to 24.0psi @08:27:13.76 SS.reconfigure forSampling @08:27:13.79 SS.seek 25.00ml @08:27:14.49 Vacuum too high -- waiting up to 40s @08:27:14.97 Intake @46.5psia, vacuum from 20.0 to 24.0psi @08:27:14.98 SS.reconfigure forSampling @08:27:15.02 SS.seek 25.00ml @08:27:15.80 Vacuum too high -- waiting up to 40s @08:27:16.29 SS.seek 25.00ml @08:27:16.99 Vacuum too high -- waiting up to 40s @08:27:17.47 Intake @46.7psia, vacuum from 20.0 to 24.0psi @08:27:17.48 SS.reconfigure forSampling @08:27:17.51 SS.seek 25.00ml @08:27:18.30 Vacuum too high -- waiting up to 40s @08:27:18.78 Intake @47.8psia, vacuum from 20.0 to 24.0psi @08:27:18.79 SS.reconfigure forSampling @08:27:18.82 SS.seek 25.00ml @08:27:19.55 Vacuum too high -- waiting up to 40s @08:27:20.02 Intake @47.4psia, vacuum from 20.0 to 24.0psi @08:27:20.03 SS.reconfigure forSampling @08:27:20.06 SS.seek 25.00ml @08:27:20.77 Vacuum too high -- waiting up to 40s @08:27:21.03 Intake @46.7psia, vacuum from 20.0 to 24.0psi @08:27:21.04 SS.reconfigure forSampling @08:27:21.07 SS.seek 25.00ml @08:27:21.68 Vacuum too high -- waiting up to 40s @08:27:22.15 Intake @46.3psia, vacuum from 20.0 to 24.0psi @08:27:22.16 SS.reconfigure forSampling @08:27:22.19 SS.seek 25.00ml @08:27:22.96 Vacuum too high -- waiting up to 40s @08:27:23.44 Intake @46.4psia, vacuum from 20.0 to 24.0psi @08:27:23.45 SS.reconfigure forSampling @08:27:23.48 SS.seek 25.00ml @08:27:24.24 Vacuum too high -- waiting up to 40s @08:27:24.72 Intake @46.5psia, vacuum from 20.0 to 24.0psi @08:27:24.73 SS.reconfigure forSampling @08:27:24.76 SS.seek 25.00ml @08:27:25.43 Vacuum too high -- waiting up to 40s @08:27:25.69 Intake @46.5psia, vacuum from 20.0 to 24.0psi @08:27:25.70 SS.reconfigure forSampling @08:27:25.74 SS.seek 25.00ml @08:27:26.30 Vacuum too high -- waiting up to 40s @08:27:26.78 Intake @46.0psia, vacuum from 20.0 to 24.0psi @08:27:26.79 SS.reconfigure forSampling @08:27:26.82 SS.seek 25.00ml @08:27:27.62 Vacuum too high -- waiting up to 40s @08:27:28.10 Intake @45.8psia, vacuum from 20.0 to 24.0psi @08:27:28.11 SS.reconfigure forSampling @08:27:28.14 SS.seek 25.00ml @08:27:29.02 Vacuum too high -- waiting up to 40s @08:27:29.49 Intake @46.7psia, vacuum from 20.0 to 24.0psi @08:27:29.50 SS.reconfigure forSampling @08:27:29.53 SS.seek 25.00ml @08:27:30.30 Vacuum too high -- waiting up to 40s @08:27:30.77 Intake @47.4psia, vacuum from 20.0 to 24.0psi @08:27:30.78 SS.reconfigure forSampling @08:27:30.81 SS.seek 25.00ml @08:27:31.52 Vacuum too high -- waiting up to 40s @08:27:31.78 Intake @47.3psia, vacuum from 20.0 to 24.0psi @08:27:31.79 SS.reconfigure forSampling @08:27:31.82 SS.seek 25.00ml @08:27:32.37 Vacuum too high -- waiting up to 40s @08:27:32.85 Intake @46.9psia, vacuum from 20.0 to 24.0psi @08:27:32.86 SS.reconfigure forSampling @08:27:32.89 SS.seek 25.00ml @08:27:33.68 Vacuum too high -- waiting up to 40s @08:27:33.95 SS.seek 25.00ml @08:27:34.52 Vacuum too high -- waiting up to 40s @08:27:35.37 Intake @46.8psia, vacuum from 20.0 to 24.0psi @08:27:35.38 SS.reconfigure forSampling @08:27:35.42 SS.seek 25.00ml @08:27:36.40 Vacuum too high -- waiting up to 40s @08:27:36.88 Intake @46.6psia, vacuum from 20.0 to 24.0psi @08:27:36.89 SS.reconfigure forSampling @08:27:36.93 SS.seek 25.00ml @08:27:37.77 Vacuum too high -- waiting up to 40s @08:27:38.25 Intake @46.5psia, vacuum from 20.0 to 24.0psi @08:27:38.26 SS.reconfigure forSampling @08:27:38.30 SS.seek 25.00ml @08:27:39.05 Vacuum too high -- waiting up to 40s @08:27:39.53 Intake @46.4psia, vacuum from 20.0 to 24.0psi @08:27:39.54 SS.reconfigure forSampling @08:27:39.58 SS.seek 25.00ml @08:27:40.30 Vacuum too high -- waiting up to 40s @08:27:40.78 Intake @46.5psia, vacuum from 20.0 to 24.0psi @08:27:40.79 SS.reconfigure forSampling @08:27:40.83 SS.seek 25.00ml @08:27:41.62 Vacuum too high -- waiting up to 40s @08:27:42.10 Intake @46.7psia, vacuum from 20.0 to 24.0psi @08:27:42.11 SS.reconfigure forSampling @08:27:42.14 SS.seek 25.00ml @08:27:42.90 Vacuum too high -- waiting up to 40s @08:27:43.39 Intake @47.2psia, vacuum from 20.0 to 24.0psi @08:27:43.40 SS.reconfigure forSampling @08:27:43.43 SS.seek 25.00ml @08:27:44.27 Vacuum too high -- waiting up to 40s @08:27:44.74 Intake @47.6psia, vacuum from 20.0 to 24.0psi @08:27:44.75 SS.reconfigure forSampling @08:27:44.79 SS.seek 25.00ml @08:27:45.55 Vacuum too high -- waiting up to 40s @08:27:46.03 Intake @47.1psia, vacuum from 20.0 to 24.0psi @08:27:46.04 SS.reconfigure forSampling @08:27:46.08 SS.seek 25.00ml @08:27:46.83 Vacuum too high -- waiting up to 40s @08:27:47.31 Intake @46.5psia, vacuum from 20.0 to 24.0psi @08:27:47.32 SS.reconfigure forSampling @08:27:47.36 SS.seek 25.00ml @08:27:48.15 Vacuum too high -- waiting up to 40s @08:27:48.42 Intake @46.3psia, vacuum from 20.0 to 24.0psi @08:27:48.43 SS.reconfigure forSampling @08:27:48.46 SS.seek 25.00ml @08:27:49.08 Vacuum too high -- waiting up to 40s @08:27:49.55 Intake @46.0psia, vacuum from 20.0 to 24.0psi @08:27:49.56 SS.reconfigure forSampling @08:27:49.59 SS.seek 25.00ml @08:27:50.43 Vacuum too high -- waiting up to 40s @08:27:50.90 Intake @46.2psia, vacuum from 20.0 to 24.0psi @08:27:50.91 SS.reconfigure forSampling @08:27:50.94 SS.seek 25.00ml @08:27:51.47 SS.reconfigure SSconfig @08:27:51.50 SS.hold @08:27:51.65 Intake.select :close @08:27:51.80 ShallowSampler::Clogged in MAIN -- progress too slow after 222.2ml SSV.dial :exhaust @08:27:52.58 Exhaust.select :open @08:27:52.69 SS.reconfigure SSexhale @08:27:52.73 SS.seek 0.00ml @08:28:01.03 Exhaust.select :close @08:28:01.13 Sampled 222.2ml @08:28:01.14 collectSample: Complete Evacuating Puck (bubblePt=55.0psi, delay=15.0s) @08:28:01.15 Intake.select :close @08:28:01.21 CSV.dial :waste @08:28:01.24 CS.seek 0.000ml @08:28:01.33 SSV.dial CBV,avoiding => exhaust @08:28:01.35
CBV.dial SSV @08:28:01.58 CSV.dial :air @08:28:02.41 CS.seek 10.000ml @08:28:03.32
CTV.dial :air @08:28:04.09 delay 15 seconds @08:28:04.12 Sleepy.power :sampler,:OFF @08:28:19.09
SSV.dial :spit,avoiding => exhaust @08:28:20.43 CTV.dial CSV,avoiding => intake @08:28:20.45
SS.seek 0.00ml @08:28:20.55 SSV.dial CBV,avoiding => exhaust @08:28:27.00 CBV.dial between CSV and waste2b @08:28:27.92 CTV.dial CSV,avoiding => air @08:28:27.94
CSV.dial CTV @08:28:28.38 CS.reconfigure CSpressurize @08:28:28.41 CS.seek 0.000ml @08:28:52.62 BubblePoint: Intake @70.2psia, Outlet @14.6psia @08:28:52.63 SS.reconfigure SSconfig @08:28:52.66 SS.seek 10.00ml @08:28:58.13 delay 15 seconds @08:29:13.85 Evacuated: Intake @69.5psia, Outlet @-0.1psia @08:29:13.86 SSV.dial :spit,avoiding => exhaust @08:29:15.18 SS.reconfigure SSexhale @08:29:15.21 SS.seek 0.00ml @08:29:20.04 SS.reconfigure SSfast @08:29:20.08 SS.seek 5.00ml @08:29:22.74 SSV.dial CBV,avoiding => exhaust @08:29:22.76
CBV.dial SSV @08:29:24.12 CTV.dial :air,avoiding => intake @08:29:25.43 CSV.dial CTV @08:29:25.44
SS.reconfigure SSconfig @08:29:25.48 SS.seek 10.00ml @08:29:26.44 CSV.dial :air @08:29:26.97 CS.reconfigure CSfast @08:29:27.00 CS.seek 0.000ml @08:29:28.59
SSV.dial :spit,avoiding => exhaust @08:29:29.32 CS.coast @08:29:29.91
SS.reconfigure SSexhale @08:29:29.94 SS.seek 0.00ml @08:29:35.04 SS.reconfigure SSfast @08:29:35.07 SS.seek 5.00ml @08:29:37.57 SSV.dial CBV,avoiding => exhaust @08:29:37.59
CBV.dial SSV @08:29:39.90 SSV.dial :spit,avoiding => exhaust @08:29:41.22 SS.reconfigure SSconfig @08:29:41.25 SS.seek 0.00ml @08:29:44.39 SS.coast @08:29:44.42 Evacuating Puck (bubblePt=55.0psi, delay=15.0s) Intake.select :close @08:29:44.47 CSV.dial :waste @08:29:44.56 SSV.dial CBV,avoiding => exhaust @08:29:44.58
CBV.dial SSV @08:29:45.21 CS.seek 0.000ml @08:29:45.45 CSV.dial :air @08:29:45.91
CTV.dial :air @08:29:45.92 delay 15 seconds @08:29:46.24 CS.seek 10.000ml @08:30:00.92
SSV.dial :spit,avoiding => exhaust @08:30:02.24 CTV.dial CSV,avoiding => intake @08:30:02.26
SS.reconfigure SSexhale @08:30:02.32 SS.seek 0.00ml @08:30:02.67 SSV.dial CBV,avoiding => exhaust @08:30:10.28 CBV.dial between CSV and waste2b @08:30:11.23 CSV.dial between air and CTV @08:30:12.13 CS.seek 7.274ml @08:30:19.14 CTV.dial CSV,avoiding => air @08:30:19.15
CSV.dial CTV @08:30:19.52 CS.reconfigure CSpressurize @08:30:19.55 CS.seek 0.000ml @08:30:38.51 CSV.dial :air @08:30:39.02 CS.reconfigure CSfast @08:30:39.05 CS.seek 10.000ml @08:31:02.89 CSV.dial between air and CTV @08:31:03.26 CS.seek 2.201ml @08:31:21.83 CTV.dial CSV,avoiding => air @08:31:21.84
CSV.dial CTV @08:31:22.15 CS.reconfigure CSpressurize @08:31:22.18 CS.seek 0.000ml @08:31:24.24 BubblePoint: Intake @73.4psia, Outlet @18.0psia @08:31:24.25 SS.reconfigure SSconfig @08:31:24.28 SS.seek 10.00ml @08:31:30.19 delay 15 seconds @08:31:45.29 Evacuated: Intake @72.6psia, Outlet @-0.2psia SSV.dial :spit,avoiding => exhaust @08:31:46.62 SS.reconfigure SSexhale @08:31:46.65 SS.seek 0.00ml @08:31:51.58 SS.reconfigure SSfast @08:31:51.61 SS.seek 5.00ml @08:31:54.23 SSV.dial CBV,avoiding => exhaust @08:31:54.25
CBV.dial SSV @08:31:55.58 CTV.dial :air,avoiding => intake @08:31:56.88 CSV.dial CTV @08:31:56.90
SS.reconfigure SSconfig @08:31:56.94 SS.seek 10.00ml @08:31:57.90 CSV.dial :air @08:31:58.42 CS.reconfigure CSfast @08:31:58.45 CS.seek 0.000ml @08:32:00.11
SSV.dial :spit,avoiding => exhaust @08:32:01.42 SS.reconfigure SSexhale @08:32:01.45 SS.seek 0.00ml @08:32:02.94 CS.coast @08:32:06.75
SS.reconfigure SSfast @08:32:06.79 SS.seek 5.00ml @08:32:09.28 SSV.dial CBV,avoiding => exhaust @08:32:09.30
CBV.dial SSV @08:32:11.61 SSV.dial :spit,avoiding => exhaust @08:32:12.93 SS.reconfigure SSconfig @08:32:12.96 SS.seek 0.00ml @08:32:16.11 SS.coast @08:32:16.14 Lysing: Adding 1.5ml lysis CBV.dial between CSV and waste1,avoiding => DBV @08:32:17.61 CTV.dial CSV @08:32:18.88 CSV.dial CSR @08:32:20.25 CSR.select :lysis @08:32:20.35 CS.reconfigure CSconfig @08:32:20.39 CS.seek 1.000ml @08:32:23.75 delay 5 seconds @08:32:28.76 CSR.select :air @08:32:28.90 CS.seek 6.000ml @08:32:42.34 delay 2 seconds @08:32:44.36 CSV.dial :waste1 @08:32:45.62 CS.reconfigure CSfast @08:32:45.65 CS.seek empty @08:33:00.06 CSV.dial CSR @08:33:01.55 CSR.select :lysis @08:33:01.65 CS.reconfigure CSconfig @08:33:01.69 CS.seek 1.500ml @08:33:06.31 delay 5 seconds @08:33:11.32 CSR.select :air @08:33:11.46 CS.seek 4.500ml @08:33:19.87 delay 2 seconds @08:33:21.89 CSV.dial CTV @08:33:22.32 CS.seek 1.275ml @08:33:31.26 Heating sh1 puck from tube 2 in Collection Clamp to 85.0C for 5:00 @08:33:31.27 CH.seek 85.0C,8:00 @08:36:03.21 CH.hold 5:00,85.0C @08:41:03.32 Collection Puck Heater at 84.9C after lysing @08:41:03.33 CS.seek 0.000ml @08:41:08.08 CTV.dial between spare and air,avoiding => intake @08:41:09.07 CSV.dial :air @08:41:09.72 CS.seek 5.000ml @08:41:23.20 CSV.dial CTV @08:41:23.62 delay 2 seconds @08:41:25.63 CSV.dial :air @08:41:26.15 delay 2 seconds @08:41:28.16 CSV.dial CTV @08:41:28.68 delay 2 seconds @08:41:30.69 CSV.dial :air @08:41:31.21 delay 2 seconds @08:41:33.22 CS.seek 0.000ml @08:41:46.70 Recovering Lysate into Collection syringe @08:41:46.71 CSV.dial CBV @08:41:47.70 CBV.dial CSV @08:41:48.06 CS.seek 5.000ml @08:42:01.48 delay 5 seconds @08:42:06.49 CTV.dial :air @08:42:06.98 CS.seek 7.001ml @08:42:12.94 delay 5 seconds @08:42:17.95 CSV.dial :air2 @08:42:18.38 CS.seek 10.000ml @08:42:26.80 CSV.dial between air and CTV,avoiding => CBV @08:42:27.95 Lysing Complete @08:42:28.00 Storing sh1 puck in Collection Clamp to tube 1 @08:42:28.13 SC.reconfigure SCtight @08:42:28.17 SC.seek 1 @08:42:30.36 SC.reconfigure SCloose @08:42:30.53 Elbow.reconfigure TightElbow @08:42:30.57 Elbow.seek 1 @08:42:32.17 Forearm.reconfigure TightForearm @08:42:32.20 Forearm.seek 1 + 800 counts @08:42:33.28 SE.reconfigure SEpick @08:42:33.33 SE.seek beamBreak @08:42:34.36 SE.configure SEconfig @08:42:34.48 Elbow.reconfigure LimpElbow @08:42:34.52 Forearm.reconfigure LooseForearm @08:42:34.56 Forearm.seek 1 @08:42:34.78 Elbow.hold @08:42:34.83 Elbow.reconfigure TightElbow @08:42:34.91 SE.seek 20.05 Pucks below Top Plate @08:42:35.25 Forearm.coast @08:42:35.28 Forearm.seek 1 + 327 counts @08:42:35.51 Forearm.reconfigure TightForearm @08:42:35.55 Forearm.seek 1 + 800 counts @08:42:35.84 SE.seek 21.05 Pucks below Top Plate @08:42:36.37 Forearm.reconfigure LooseForearm @08:42:36.40 Forearm.seek retract @08:42:37.32 Elbow.seek CC @08:42:38.82 Elbow.reconfigure LooseElbow @08:42:38.91 Forearm.seek CC @08:42:39.90 CC.seek sealed @08:42:40.80 Hand.grip @08:42:41.48 CC.seek open @08:42:44.83 Forearm.seek retract @08:42:45.76 Elbow.reconfigure TightElbow @08:42:45.80 Elbow.seek 1 @08:42:47.38 Elbow.reconfigure LimpElbow @08:42:47.42 Forearm.seek 1 @08:42:48.39 Elbow.hold @08:42:48.44 Elbow.reconfigure TightElbow @08:42:48.47 Hand.release @08:42:49.31 Forearm.coast @08:42:49.34 Forearm.seek 1 + 334 counts @08:42:49.57 Forearm.reconfigure TightForearm @08:42:49.60 Forearm.seek 1 + 800 counts @08:42:49.91 SE.seek down @08:42:50.58 SE.coast @08:42:51.02
Forearm.reconfigure LooseForearm @08:42:51.05 Forearm.seek retract @08:42:51.97 SC.coast @08:42:52.00 Tranferring lysate to PRV CSV.dial :air @08:42:52.29 CS.seek 10.000ml @08:42:52.36 CSV.dial between air and CTV @08:42:53.24 AIV.dial :bypass @08:42:53.31 Beginning samplePassC @08:42:53.32 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:42:53.46 Elbow.seek FlushPuck::Garage @08:42:55.25 Elbow.reconfigure LooseElbow @08:42:55.33 Forearm.seek garage @08:42:56.34 Hand.grip @08:42:57.19 Forearm.seek retract @08:42:58.23 Elbow.reconfigure TightElbow @08:42:58.26 Elbow.seek CC @08:43:01.07 Elbow.reconfigure LooseElbow @08:43:01.16 Forearm.reconfigure TightForearm @08:43:01.19 Forearm.seek CC @08:43:02.28 CC.seek closed @08:43:06.01 Collection Clamp onSpring/offSpring for 5/22 samples, on/off torque 2.00 @08:43:06.06 Hand.release @08:43:06.89 Forearm.reconfigure LooseForearm @08:43:06.92 Forearm.seek retract @08:43:07.86 AWV.select :air @08:43:07.87 AIV.dial :bypass @08:43:07.88 PSV.dial PSR @08:43:09.15 PSR.select :flush @08:43:09.25 PS.seek 1.500ml @08:43:25.28 delay 5 seconds @08:43:30.29 PSV.dial :waste1 @08:43:30.85 PS.seek 0.000ml @08:43:46.93 PSV.dial PSR @08:43:47.36 PSR.select :air @08:43:47.46 PS.seek 2.500ml @08:44:13.66 PRV.dial CBV @08:44:13.68
CBV.dial PRV @08:44:14.46 PSV.dial :mixloop @08:44:15.46 CTV.dial :air,avoiding => intake @08:44:15.47 PTV.dial :PRVmixing @08:44:15.55 PS.seek 0.000ml @08:44:41.84 PSV.dial :air @08:44:42.29 PS.seek 2.500ml @08:45:08.56 PSV.dial :mixloop @08:45:09.00 PS.seek 0.000ml @08:45:35.24 PSV.dial :air @08:45:35.82 PS.seek 2.500ml @08:46:02.09 PSV.dial :mixloop @08:46:02.52 PS.seek 0.000ml @08:46:28.83 Garaging FlushPuck in Collection Clamp @08:46:28.89 Elbow.reconfigure TightElbow @08:46:28.92 Elbow.seek CC @08:46:29.01 Elbow.reconfigure LooseElbow @08:46:29.10 Forearm.seek CC @08:46:30.08 CC.seek sealed @08:46:31.01 Hand.grip @08:46:31.72 CC.seek open @08:46:35.06 Forearm.seek retract @08:46:36.02 Elbow.reconfigure TightElbow @08:46:36.06 Elbow.seek garage @08:46:38.90 Elbow.reconfigure LooseElbow @08:46:38.99 Forearm.reconfigure TightForearm @08:46:39.02 Forearm.seek garage @08:46:40.21 Hand.release @08:46:41.01 Forearm.reconfigure LooseForearm @08:46:41.04 Forearm.seek retract @08:46:42.07 Connecting PS to CS to transfer sample @08:46:42.08 CBV.dial AIV @08:46:42.09 AIV.dial :bypass @08:46:42.10 PSV.dial :mixloop @08:46:42.11 PTV.dial :PRVmixing PRV.dial AIV @08:46:42.12 CTV.dial :air,avoiding => intake @08:46:42.21 Loading lyfil puck from tube 3 into Collection Clamp @08:46:42.38 SC.reconfigure SCtight @08:46:42.41 SC.seek 3 @08:46:46.35 SC.reconfigure SCloose @08:46:46.52 Elbow.reconfigure TightElbow @08:46:46.55 Elbow.seek 3 @08:46:48.39 Forearm.reconfigure TightForearm @08:46:48.42 Forearm.seek 3 + 800 counts @08:46:49.49 SE.reconfigure SEpick @08:46:49.54 SE.seek beamBreak @08:46:50.66 SE.configure SEconfig @08:46:50.78 Elbow.seek 3 @08:46:50.93 Forearm.seek 3 + 300 counts @08:46:51.16 Forearm.reconfigure LooseForearm @08:46:51.20 Hand.grip @08:46:51.98 SE.seek down @08:46:52.12
Forearm.seek retract @08:46:52.82 SE.coast @08:46:53.08
Elbow.seek CC @08:46:54.64 Elbow.reconfigure LooseElbow @08:46:54.73 Forearm.reconfigure TightForearm @08:46:54.76 Forearm.seek CC @08:46:55.89 CC.seek closed @08:46:59.65 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.97 @08:46:59.66 Hand.release @08:47:00.38 Forearm.reconfigure LooseForearm @08:47:00.41 Forearm.seek retract @08:47:01.39 SC.coast @08:47:01.41 CTV.dial CSV,avoiding => intake @08:47:02.71 CSV.dial CTV @08:47:03.01 CS.seek 5.000ml @08:47:16.44 delay 1:20 @08:48:36.45 CS.seek 2.500ml @08:48:43.53 delay 40 seconds @08:49:23.54 CS.seek 0.000ml @08:49:30.72 delay 1:00 @08:50:30.73 PSV.dial :air @08:50:31.18 PS.seek 2.500ml @08:50:57.39 PSV.dial :mixloop @08:50:57.82 delay 3 seconds @08:51:00.83 PSV.dial :air @08:51:01.39 delay 2 seconds @08:51:03.40 PSV.dial :mixloop @08:51:03.82 delay 3 seconds @08:51:06.83 PSV.dial :waste1 @08:51:08.12 PS.seek empty @08:51:34.35 CBV.dial :waste1 @08:51:34.82 delay 5 seconds @08:51:39.83 CBV.dial AIV @08:51:40.29 delay 10 seconds @08:51:50.30 CBV.dial :waste1 @08:51:50.77 delay 10 seconds @08:52:00.78 CBV.dial AIV @08:52:01.23 delay 10 seconds @08:52:11.24 CTV.dial CSV,avoiding => intake @08:52:11.25 CBV.dial between AIV and waste1 @08:52:11.58 CSV.dial :air @08:52:12.11 CS.seek 10.000ml @08:52:38.21 CSV.dial CTV @08:52:38.73 CS.seek 0.000ml @08:53:04.83 CBV.dial :waste1 @08:53:05.13 delay 10 seconds @08:53:15.14 CTV.dial :air,avoiding => intake @08:53:16.47 Storing lyfil puck in Collection Clamp to tube 2 @08:53:16.60 SC.reconfigure SCtight @08:53:16.63 SC.seek 2 @08:53:18.77 SC.reconfigure SCloose @08:53:18.89 Elbow.reconfigure TightElbow @08:53:18.93 Elbow.seek CC @08:53:18.95 SE.reconfigure SEplace @08:53:19.03 SE.seek beamBreak @08:53:19.18
Elbow.reconfigure LooseElbow @08:53:19.27 Forearm.seek CC @08:53:20.22 CC.seek sealed @08:53:21.14 Hand.grip @08:53:21.82 SE.configure SEconfig @08:53:21.86
CC.seek open @08:53:21.88 SE.seek place @08:53:25.24
Forearm.seek retract @08:53:26.19 Elbow.reconfigure TightElbow @08:53:26.23 Elbow.seek 2 @08:53:27.78 Elbow.reconfigure LimpElbow @08:53:27.81 Forearm.seek 2 @08:53:28.81 Elbow.hold @08:53:28.86 Elbow.reconfigure TightElbow @08:53:28.90 Hand.release @08:53:30.28 Forearm.coast @08:53:30.31 Forearm.seek 2 + 344 counts @08:53:30.55 Forearm.reconfigure TightForearm @08:53:30.59 Forearm.seek 2 + 800 counts @08:53:30.94 SE.seek down @08:53:32.05
Forearm.reconfigure LooseForearm @08:53:32.09 Forearm.seek retract @08:53:33.62 SE.coast @08:53:33.65
SC.coast @08:53:33.67 PSV.dial :air @08:53:34.73 PS.seek 0.499ml @08:53:40.66 delay 3 seconds @08:53:43.67 PSV.dial :mixloop @08:53:44.18 PRV.dial :air @08:53:44.62 delay 3 seconds @08:53:47.63 PRV.dial CBV @08:53:48.07 CBV.dial AIV @08:53:48.52 PRV.dial between CBV and air @08:53:48.82 PRV.dial :air @08:53:49.15 PS.reconfigure PSslow @08:53:49.19 PS.seek 0.599ml @08:53:51.76 PRV.dial :waste2 @08:53:52.48 PS.reconfigure PSconfig @08:53:52.51 PS.seek empty @08:53:59.44 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:53:59.58 Elbow.seek FlushPuck::Garage @08:54:01.24 Elbow.reconfigure LooseElbow @08:54:01.33 Forearm.seek garage @08:54:02.38 Hand.grip @08:54:03.15 Forearm.seek retract @08:54:04.16 Elbow.reconfigure TightElbow @08:54:04.20 Elbow.seek CC @08:54:06.98 Elbow.reconfigure LooseElbow @08:54:07.07 Forearm.reconfigure TightForearm @08:54:07.10 Forearm.seek CC @08:54:08.19 CC.seek closed @08:54:11.98 Collection Clamp onSpring/offSpring for 4/21 samples, on/off torque 2.00 @08:54:12.03 Hand.release @08:54:12.86 Forearm.reconfigure LooseForearm @08:54:12.89 Forearm.seek retract @08:54:13.86 Clearing PML for modified lysate CTV.dial :air,avoiding => intake @08:54:13.87 PRV.dial :waste2 @08:54:13.88 PTV.dial :PRV,avoiding => puck @08:54:14.60 delay 2 seconds @08:54:16.61 PTV.dial :PRVmixing,avoiding => puck @08:54:17.35 delay 2 seconds @08:54:19.36 PSV.dial :PML @08:54:19.37 delay 2 seconds @08:54:21.37 PRV.dial :air @08:54:22.08 delay 2 seconds @08:54:24.14 PS.seek 1.499ml @08:54:40.25 delay 5 seconds @08:54:45.26 PS.seek 2.500ml @08:54:56.19 PSV.dial :air @08:54:56.65 PS.seek 0.000ml @08:55:22.91 mixing lysate with speDil @08:55:22.92 PSV.dial :PML @08:55:23.45 CBV.dial :PRV @08:55:23.46 PRV.dial :air @08:55:23.47 delay 2 seconds @08:55:25.47 PRV.dial :speDil @08:55:25.92 delay 2 seconds @08:55:27.98 PS.reconfigure PSslow1 @08:55:28.01 PS.seek 0.225ml @08:55:51.35 delay 5 seconds @08:55:56.36 PRV.dial CBV @08:55:57.10 delay 2 seconds @08:55:59.16 PS.seek 0.475ml @08:56:24.91 delay 5 seconds @08:56:29.93 PRV.dial :air @08:56:30.36 delay 2 seconds @08:56:32.37 PS.seek 0.925ml @08:57:18.37 delay 10 seconds @08:57:28.38 PRV.dial CBV @08:57:28.81 CTV.dial :air,avoiding => intake @08:57:28.82 PSV.dial :air @08:57:29.27 PS.reconfigure PSconfig @08:57:29.30 PS.seek 2.500ml @08:57:46.06 delay 3 seconds @08:57:49.07 PSV.dial PTV @08:57:49.79 PTV.dial :PRV,avoiding => puck @08:57:51.15 PS.seek 0.000ml @08:58:17.37 PSV.dial :air @08:58:18.23 PS.seek 2.500ml @08:58:44.53 delay 3 seconds @08:58:47.54 PSV.dial PTV @08:58:48.25 PTV.dial :PRV,avoiding => puck @08:58:48.27 PS.seek 0.000ml @08:59:14.53 Pushing modified lysate into AIV Injection Loop @08:59:14.54 CBV.dial AIV @08:59:14.55 AIV.dial :bypass @08:59:14.56 PRV.dial CBV @08:59:14.57 PSV.dial :air @08:59:15.30 PS.seek 2.500ml @08:59:41.60 delay 3 seconds @08:59:44.61 PSV.dial :PML @08:59:45.05 PTV.dial :PRVmixing,avoiding => puck @08:59:45.82 PS.reconfigure PSslow1 @08:59:45.85 PS.seek 1.850ml @09:00:52.38 delay 5 seconds @09:00:57.39 AIV.dial :sample @09:00:57.92 AWV.select :waste @09:00:58.08 PS.reconfigure PSslow2 @09:00:58.11 PS.seek 1.310ml @09:02:48.12 delay 15 seconds @09:03:03.13 AIV.dial :bypass @09:03:03.69 PS.reconfigure PSconfig @09:03:03.72 PS.seek 0.000ml @09:03:17.84 PSV.dial :air @09:03:18.29 PS.seek 2.500ml @09:03:44.56 PSV.dial :PML @09:03:45.00 PS.seek 0.000ml @09:04:11.24 AIV.dial :coilToMFB @09:04:11.25 AWV.select :air @09:04:11.39 Extracting Nucleic Acid from AIV @09:04:11.41
Beginning samplePassD @09:04:11.42 SPE.hold 2:46:40,55.0C @09:04:11.44 ASV.dial :SPEdelivery @09:04:11.57
AWV.select :air @09:04:11.58 AIV.dial :bypass PSV.dial PSR @09:04:11.99 delay 20 seconds @09:04:12.62
PSR.select :killMFB @09:04:12.72 PS.seek 1.500ml @09:04:28.84 delay 5 seconds @09:04:32.00 primeLysateINT @09:04:32.01 AWV.select :air @09:04:32.02 AIV.dial :coilToMFB ATV.dial :AIV @09:04:33.51 AS.reconfigure ASslow1 @09:04:33.54 AS.seek 0.239ml @09:04:33.85
PSV.dial :waste1 @09:04:34.30 PS.seek 0.000ml @09:04:50.37 PSV.dial PSR @09:04:50.82 PSR.select :air @09:04:50.92 PS.seek 2.500ml @09:05:17.21 PRV.dial CBV @09:05:17.23
CBV.dial PRV @09:05:17.24 PSV.dial :mixloop @09:05:18.27 CTV.dial :air,avoiding => intake @09:05:18.28 PTV.dial :PRVmixing @09:05:18.30 PS.seek 0.000ml @09:05:22.75 delay 10 seconds @09:05:32.76 ATV.dial :air2 @09:05:33.35 AS.seek 0.269ml @09:05:39.70 ATV.dial :waste2 @09:05:40.06 AS.reconfigure ASnorm @09:05:40.09 AS.seek empty @09:05:44.55
PSV.dial PSR @09:05:45.58 PSR.select :flush @09:05:45.68 PS.seek 1.500ml @09:05:54.25 Lysate primed! @09:05:54.26 AFV.select :holdingCoil @09:05:54.27 ATV.dial :air2 @09:05:54.65 AS.reconfigure ASslow1 @09:05:54.69 AS.seek 0.069ml @09:06:01.83
delay 5 seconds @09:06:06.84 PSV.dial :waste1 @09:06:07.28 PS.seek 0.000ml @09:06:09.28 delay 3 seconds @09:06:12.29 ATV.dial :AIV @09:06:12.88 AS.seek 0.469ml @09:06:23.30
PSV.dial PSR @09:06:23.75 PSR.select :air @09:06:23.85 PS.seek 2.500ml @09:06:50.08 PRV.dial CBV @09:06:50.10
CBV.dial PRV @09:06:50.11 PSV.dial :mixloop @09:06:51.13 CTV.dial :air,avoiding => intake @09:06:51.14 PTV.dial :PRVmixing @09:06:51.16 PS.seek 0.000ml @09:07:17.42 PSV.dial PSR @09:07:18.44 PSR.select :flush @09:07:18.54 PS.seek 1.500ml @09:07:34.58 delay 5 seconds @09:07:34.70 delay 5 seconds @09:07:39.59
PSV.dial :waste1 @09:07:39.75 Solid Phase Extraction Heater at 51.8C @09:07:39.76 ATV.dial ASV @09:07:40.04
PS.seek 0.000ml @09:07:40.09 ASV.dial :SPEdelivery @09:07:40.15 AS.seek 0.041ml @09:07:56.08
PSV.dial PSR @09:07:56.53 PSR.select :air @09:07:56.63 PS.seek 2.500ml @09:08:22.83 PRV.dial CBV @09:08:23.43
CBV.dial PRV @09:08:23.44 PSV.dial :mixloop @09:08:24.44 CTV.dial :air,avoiding => intake @09:08:24.45 PTV.dial :PRVmixing @09:08:24.47 PS.seek 0.000ml @09:08:50.73 PSV.dial PSR @09:08:51.75 PSR.select :flush @09:08:51.85 PS.seek 1.500ml @09:09:07.70 delay 10 seconds @09:09:07.92
delay 5 seconds @09:09:12.93 PSV.dial :waste1 @09:09:13.37 PS.seek 0.000ml @09:09:17.71 rinseLysateLineINT @09:09:17.72 AFV.select :carrierSolution @09:09:17.81 delay 2 seconds @09:09:19.82 AS.reconfigure ASconfig @09:09:19.85 AS.seek 1.000ml @09:09:29.48
PSV.dial PSR @09:09:29.92 PSR.select :air @09:09:30.02 PS.seek 2.500ml @09:09:56.23 PRV.dial CBV @09:09:56.25
CBV.dial PRV @09:09:56.26 PSV.dial :mixloop @09:09:57.26 CTV.dial :air,avoiding => intake @09:09:57.27 PTV.dial :PRVmixing @09:09:57.28 PS.reconfigure PSslow @09:09:57.32 PS.seek 1.200ml @09:10:15.99 delay 1 second @09:10:17.00 AFV.select :holdingCoil @09:10:17.09 delay 1 second @09:10:18.10 ATV.dial :AIV @09:10:18.44 AIV.dial :coilToMFB @09:10:18.45 AWV.select :waste @09:10:18.55 AS.reconfigure ASslow0 @09:10:18.59 AS.seek 0.000ml @09:10:24.23
PSV.dial :waste1 @09:10:25.66 PS.reconfigure PSconfig @09:10:25.69 PS.seek 0.000ml @09:10:38.70 PSV.dial :air @09:10:39.71 PS.seek 2.500ml @09:11:05.95 PSV.dial :mixloop @09:11:06.39 PS.reconfigure PSslow @09:11:06.42 PS.seek 0.000ml @09:11:57.76 PSV.dial :air @09:11:58.22 PS.reconfigure PSconfig @09:11:58.25 PS.seek 2.500ml @09:12:00.93 ATV.dial :air2 @09:12:01.51 AS.reconfigure ASslow1 @09:12:01.54 AS.seek 0.050ml @09:12:12.02 ATV.dial :AIV @09:12:12.60 AS.seek 0.010ml @09:12:21.00 ATV.dial :waste @09:12:22.29 AS.seek empty @09:12:24.54
PSV.dial :mixloop @09:12:24.72 ethanol wash @09:12:24.73 ATV.dial :air2 @09:12:25.11
PS.reconfigure PSslow @09:12:25.14 PS.seek 0.000ml @09:12:25.68 AS.reconfigure ASslow2 @09:12:25.71 AS.seek 0.009ml @09:12:36.24 delay 3 seconds @09:12:39.25 Pulling 0.4ml of Ethanol Wash @09:12:39.26 ATV.dial :ethanol @09:12:39.93 AS.reconfigure ASslow1 @09:12:39.96 AS.seek 0.409ml @09:13:16.66
Cleaning Collection Syringe @09:13:16.67 CSV.dial CSR @09:13:17.09 CSR.select :flush @09:13:17.19 CS.seek 4.000ml @09:13:28.16 delay 5 seconds @09:13:33.18 CSR.select :air @09:13:33.41 CS.seek 10.000ml @09:13:49.41 CSV.dial CTV @09:13:49.43
CTV.dial CSV @09:13:50.77 CBV.dial :waste1 @09:13:51.25 CS.seek 0.000ml @09:14:01.81 delay 3 seconds @09:14:04.82 ATV.dial :air1 @09:14:05.16 delay 3 seconds @09:14:08.22 AS.seek 0.434ml @09:14:13.58 delay 3 seconds @09:14:16.59 ATV.dial :ethanol @09:14:16.99 AS.seek 0.419ml @09:14:17.35
CSV.dial CSR @09:14:17.85 CSR.select :flush @09:14:17.95 CS.seek 4.000ml @09:14:20.30 delay 3 seconds @09:14:23.31 ATV.dial ASV @09:14:24.44 AS.seek 0.092ml @09:14:28.88
delay 5 seconds @09:14:33.89 CSR.select :air @09:14:33.98 CS.seek 10.000ml @09:14:49.94 CSV.dial CTV @09:14:49.96
CTV.dial CSV @09:14:50.46 CBV.dial :waste1 @09:14:50.48 CS.seek 0.000ml @09:15:16.71 Garaging FlushPuck in Collection Clamp @09:15:16.76 Elbow.reconfigure TightElbow @09:15:16.79 Elbow.seek CC @09:15:16.88 Elbow.reconfigure LooseElbow @09:15:16.96 Forearm.seek CC @09:15:17.89 CC.seek sealed @09:15:18.82 Hand.grip @09:15:19.53 CC.seek open @09:15:23.43 Forearm.seek retract @09:15:24.38 Elbow.reconfigure TightElbow @09:15:24.42 Elbow.seek garage @09:15:27.26 Elbow.reconfigure LooseElbow @09:15:27.34 Forearm.reconfigure TightForearm @09:15:27.37 Forearm.seek garage @09:15:28.55 Hand.release @09:15:29.32 Forearm.reconfigure LooseForearm @09:15:29.35 Forearm.seek retract @09:15:30.35 Whole Cell Archive sampling 1000ml @09:15:30.37 Loading wcr puck from tube 3 into Collection Clamp @09:15:30.54 SC.reconfigure SCtight @09:15:30.58 SC.seek 3 @09:15:31.34 delay 3 seconds @09:15:32.82 SC.reconfigure SCloose @09:15:33.00 Elbow.reconfigure TightElbow @09:15:33.03 Elbow.seek 3 @09:15:34.35 ATV.dial :waste2 @09:15:34.85 Forearm.reconfigure TightForearm @09:15:34.88 Forearm.seek 3 + 800 counts @09:15:34.99 AS.reconfigure ASconfig @09:15:35.02 AS.seek empty @09:15:35.94 SE.reconfigure SEpick @09:15:35.99 SE.seek beamBreak @09:15:36.97 SE.configure SEconfig @09:15:37.10 Elbow.seek 3 @09:15:37.24 Forearm.seek 3 + 300 counts @09:15:37.50 Forearm.reconfigure LooseForearm @09:15:37.54 Hand.grip @09:15:38.30 SE.seek down @09:15:38.43 Forearm.seek retract @09:15:39.10 SE.coast @09:15:39.40 Elbow.seek CC @09:15:40.72 ATV.dial :air2 @09:15:40.90 Elbow.reconfigure LooseElbow @09:15:40.98 Forearm.reconfigure TightForearm @09:15:41.01 Forearm.seek CC @09:15:41.09 AS.reconfigure ASslow1 @09:15:41.12 AS.seek 1.000ml @09:15:42.07 CC.seek closed @09:15:45.83 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.91 @09:15:45.84 Hand.release @09:15:46.57 Forearm.reconfigure LooseForearm @09:15:46.60 Forearm.seek retract @09:15:47.55 SC.coast @09:15:47.58 collectSample 1000ml w/bubble point of 55psi @09:15:47.60 Priming 7ml Sleepy.power :sampler,:ON @09:15:47.61 Intake.select :close @09:15:47.62 Exhaust.select :close @09:15:47.63 SS.hold @09:15:47.78 Calibrating pressure sensors @09:15:47.79 CTV.dial :air @09:15:49.12 SSV.dial :spit,avoiding => exhaust @09:15:49.13 SS.reconfigure SSfast @09:15:49.16 SS.seek 5.00ml @09:15:51.77 SSV.dial CBV @09:15:51.79 CBV.dial SSV @09:15:53.20 delay 3.8 seconds @09:15:57.09 SSV.dial :spit @09:15:58.45 SS.seek 0.00ml @09:16:01.28 SSV.dial CBV @09:16:02.64 CTV.dial :intake @09:16:03.38 Waiting for external sampler to finish priming... @09:16:03.39 delayUntil "09:25:47.60" @09:19:05.55 delay 5 seconds @09:19:10.56 ATV.dial ASV @09:19:11.28 ASV.dial :SPEdelivery @09:19:11.30 AS.seek 0.020ml @09:22:31.46 delay 5 seconds @09:22:36.47 ATV.dial :air2 @09:22:37.23 AS.seek 0.520ml @09:24:19.43 delay 5 seconds @09:24:25.06 ATV.dial ASV @09:24:25.77 ASV.dial :wasteR @09:24:26.38 AS.seek 0.300ml @09:25:11.50 delay 20 seconds @09:25:31.51 AS.seek 0.070ml @09:25:47.61 SSV.dial CBV @09:25:47.63 CBV.dial SSV @09:25:47.64 Allowing 55s for 7.0ml of remaining 7.0ml @09:25:47.65 Intake.select :open @09:25:49.78 Intake @52.7psia, vacuum from 20.0 to 24.0psi @09:25:49.80 SS.reconfigure forSampling @09:25:49.83 SS.seek 7.00ml @09:25:56.12 Stroke completed -- waiting for pressure to equalize ... @09:25:56.76 Intake.select :close @09:25:56.90 SSV.dial :exhaust @09:25:57.68 Exhaust.select :open @09:25:57.78 SS.reconfigure SSexhale @09:25:57.82 SS.seek 0.00ml @09:26:01.50 Exhaust.select :close @09:26:01.61 Sampling 1000.0ml @09:26:01.62 Intake.select :close @09:26:01.63 Exhaust.select :close @09:26:01.64 SS.reconfigure SSconfig @09:26:01.67 SS.hold @09:26:01.82 CTV.dial :intake @09:26:01.83 SSV.dial CBV @09:26:01.85 CBV.dial SSV @09:26:02.65 Allowing 185s for 25.0ml of remaining 1000.0ml @09:26:02.66 Intake.select :open @09:26:04.79 Intake @51.7psia, vacuum from 20.0 to 24.0psi @09:26:04.81 SS.reconfigure forSampling @09:26:04.84 SS.seek 25.00ml @09:26:18.63 delay 20 seconds @09:26:19.71 Stroke completed -- waiting for pressure to equalize ... @09:26:20.35 Intake.select :close @09:26:20.48 SSV.dial :exhaust @09:26:21.26 Exhaust.select :open @09:26:21.37 SS.reconfigure SSexhale @09:26:21.41 SS.seek 0.00ml @09:26:30.48 Exhaust.select :close @09:26:30.59 SSV.dial CBV @09:26:30.61 CBV.dial SSV @09:26:31.40 Allowing 185s for 25.0ml of remaining 975.0ml @09:26:31.41 Intake.select :open @09:26:33.55 Intake @51.8psia, vacuum from 20.0 to 24.0psi @09:26:33.57 SS.reconfigure forSampling @09:26:33.60 SS.seek 25.00ml @09:26:38.64 ATV.dial :waste2 @09:26:39.18 AS.reconfigure ASconfig @09:26:39.21 AS.seek empty @09:26:43.64 Elution @09:26:43.65 ATV.dial :air1 @09:26:44.61 AS.reconfigure ASslow1 @09:26:44.64 AS.seek 0.049ml @09:26:46.19 Vacuum too high -- waiting up to 40s @09:26:46.46 Intake @43.8psia, vacuum from 20.0 to 24.0psi @09:26:46.47 SS.reconfigure forSampling @09:26:46.51 SS.seek 25.00ml @09:26:49.51 Stroke completed -- waiting for pressure to equalize ... @09:26:50.15 Intake.select :close @09:26:50.28 SSV.dial :exhaust @09:26:51.06 Exhaust.select :open @09:26:51.17 SS.reconfigure SSexhale @09:26:51.21 SS.seek 0.00ml @09:26:55.16 delay 3 seconds @09:26:58.17 ATV.dial :ElutionWater @09:26:58.83 AS.seek 0.109ml @09:27:00.29 Exhaust.select :close @09:27:00.39 SSV.dial CBV @09:27:00.41 CBV.dial SSV @09:27:01.21 Allowing 185s for 25.0ml of remaining 950.0ml @09:27:01.22 Intake.select :open @09:27:03.35 Intake @51.0psia, vacuum from 20.0 to 24.0psi @09:27:03.37 SS.reconfigure forSampling @09:27:03.40 SS.seek 25.00ml @09:27:11.30 delay 3 seconds @09:27:12.34 Vacuum too high -- waiting up to 40s @09:27:12.61 Intake @44.4psia, vacuum from 20.0 to 24.0psi @09:27:12.62 SS.reconfigure forSampling @09:27:12.65 SS.seek 25.00ml @09:27:14.31 ATV.dial ASV,via => air2 @09:27:15.99 ASV.dial :SPEelution @09:27:16.74 AFV.select :carrierSolution @09:27:16.89 AS.reconfigure ASnorm @09:27:16.92 AS.seek 0.759ml @09:27:21.01 Stroke completed -- waiting for pressure to equalize ... @09:27:21.65 Intake.select :close @09:27:21.78 SSV.dial :exhaust @09:27:22.57 Exhaust.select :open @09:27:22.68 SS.reconfigure SSexhale @09:27:22.72 SS.seek 0.00ml @09:27:31.60 Exhaust.select :close @09:27:31.71 SSV.dial CBV @09:27:31.73 CBV.dial SSV @09:27:32.54 Allowing 185s for 25.0ml of remaining 925.0ml @09:27:32.56 Intake.select :open @09:27:34.69 Intake @50.8psia, vacuum from 20.0 to 24.0psi @09:27:34.70 SS.reconfigure forSampling @09:27:34.74 SS.seek 25.00ml @09:27:38.67 Vacuum too high -- waiting up to 40s @09:27:38.94 Intake @45.2psia, vacuum from 20.0 to 24.0psi @09:27:38.95 SS.reconfigure forSampling @09:27:38.98 SS.seek 25.00ml @09:27:49.92 Vacuum too high -- waiting up to 40s @09:27:50.73 delay 3 seconds @09:27:50.81 Intake @49.6psia, vacuum from 20.0 to 24.0psi @09:27:50.82 SS.reconfigure forSampling @09:27:50.85 SS.seek 25.00ml @09:27:53.74 AFV.select :holdingCoil @09:27:53.88 AS.reconfigure ASslow1 @09:27:53.91 AS.seek 0.489ml @09:27:54.09 Vacuum too high -- waiting up to 40s @09:27:54.36 Intake @46.1psia, vacuum from 20.0 to 24.0psi @09:27:54.37 SS.reconfigure forSampling @09:27:54.41 SS.seek 25.00ml @09:27:58.38 Stroke completed -- waiting for pressure to equalize ... @09:27:59.02 Intake.select :close @09:27:59.17 SSV.dial :exhaust @09:27:59.96 Exhaust.select :open @09:28:00.06 SS.reconfigure SSexhale @09:28:00.10 SS.seek 0.00ml @09:28:09.15 Exhaust.select :close @09:28:09.25 SSV.dial CBV @09:28:09.27 CBV.dial SSV @09:28:10.05 Allowing 185s for 25.0ml of remaining 900.0ml @09:28:10.06 Intake.select :open @09:28:12.20 Intake @49.7psia, vacuum from 20.0 to 24.0psi @09:28:12.22 SS.reconfigure forSampling @09:28:12.25 SS.seek 25.00ml @09:28:17.05 Vacuum too high -- waiting up to 40s @09:28:17.32 Intake @47.6psia, vacuum from 20.0 to 24.0psi @09:28:17.33 SS.reconfigure forSampling @09:28:17.36 SS.seek 25.00ml @09:28:30.33 Vacuum too high -- waiting up to 40s @09:28:30.60 Intake @47.6psia, vacuum from 20.0 to 24.0psi @09:28:30.61 SS.reconfigure forSampling @09:28:30.64 SS.seek 25.00ml @09:28:47.39 Stroke completed -- waiting for pressure to equalize ... @09:28:48.61 Intake.select :close @09:28:48.74 SSV.dial :exhaust @09:28:49.22 delay 5 seconds @09:28:49.53 Exhaust.select :open @09:28:49.63 SS.reconfigure SSexhale @09:28:49.67 SS.seek 0.00ml @09:28:54.28 AS.reconfigure ASslow2 @09:28:54.31 AS.seek 0.454ml @09:28:58.85 Exhaust.select :close @09:28:58.96 SSV.dial CBV @09:28:58.98 CBV.dial SSV @09:28:59.78 Allowing 185s for 25.0ml of remaining 875.0ml @09:28:59.79 Intake.select :open @09:29:01.93 Intake @49.5psia, vacuum from 20.0 to 24.0psi @09:29:01.95 SS.reconfigure forSampling @09:29:01.98 SS.seek 25.00ml @09:29:05.76 Vacuum too high -- waiting up to 40s @09:29:06.02 Intake @48.8psia, vacuum from 20.0 to 24.0psi @09:29:06.04 SS.reconfigure forSampling @09:29:06.07 SS.seek 25.00ml @09:29:30.34 delay 1:00 @09:29:41.70 Vacuum too high -- waiting up to 40s @09:29:41.97 Intake @48.0psia, vacuum from 20.0 to 24.0psi @09:29:41.98 SS.reconfigure forSampling @09:29:42.01 SS.seek 25.00ml @09:30:17.20 Stroke completed -- waiting for pressure to equalize ... @09:30:18.42 Intake.select :close @09:30:18.55 SSV.dial :exhaust @09:30:19.36 Exhaust.select :open @09:30:19.47 SS.reconfigure SSexhale @09:30:19.51 SS.seek 0.00ml @09:30:28.36 Exhaust.select :close @09:30:28.47 SSV.dial CBV @09:30:28.49 CBV.dial SSV @09:30:29.29 Allowing 185s for 25.0ml of remaining 850.0ml @09:30:29.30 Intake.select :open @09:30:30.35 pushing eluate to holding coil @09:30:30.41 AS.seek 0.294ml @09:30:31.44 Intake @48.3psia, vacuum from 20.0 to 24.0psi @09:30:31.46 SS.reconfigure forSampling @09:30:31.49 SS.seek 25.00ml @09:32:21.66 Stroke completed -- waiting for pressure to equalize ... @09:32:24.16 Intake.select :close @09:32:24.30 SSV.dial :exhaust @09:32:25.10 Exhaust.select :open @09:32:25.21 SS.reconfigure SSexhale @09:32:25.25 SS.seek 0.00ml @09:32:34.38 Exhaust.select :close @09:32:34.49 SSV.dial CBV @09:32:34.51 CBV.dial SSV @09:32:35.30 Allowing 185s for 25.0ml of remaining 825.0ml @09:32:35.31 Intake.select :open @09:32:37.46 Intake @49.4psia, vacuum from 20.0 to 24.0psi @09:32:37.47 SS.reconfigure forSampling @09:32:37.51 SS.seek 25.00ml @09:33:07.06 Vacuum too high -- waiting up to 40s @09:33:07.33 Intake @47.9psia, vacuum from 20.0 to 24.0psi @09:33:07.34 SS.reconfigure forSampling @09:33:07.37 SS.seek 25.00ml @09:33:13.88 delay 30 seconds @09:33:22.47 Vacuum too high -- waiting up to 40s @09:33:22.96 Intake @47.6psia, vacuum from 20.0 to 24.0psi @09:33:22.97 SS.reconfigure forSampling @09:33:23.01 SS.seek 25.00ml @09:33:33.79 Vacuum too high -- waiting up to 40s @09:33:34.28 Intake @48.2psia, vacuum from 20.0 to 24.0psi @09:33:34.29 SS.reconfigure forSampling @09:33:34.33 SS.seek 25.00ml @09:33:37.79 Vacuum too high -- waiting up to 40s @09:33:38.29 Intake @47.7psia, vacuum from 20.0 to 24.0psi @09:33:38.30 SS.reconfigure forSampling @09:33:38.34 SS.seek 25.00ml @09:33:41.52 Vacuum too high -- waiting up to 40s @09:33:42.01 Intake @47.4psia, vacuum from 20.0 to 24.0psi @09:33:42.03 SS.reconfigure forSampling @09:33:42.06 SS.seek 25.00ml @09:33:44.50 ATV.dial :waste,via => air3 @09:33:46.01 AS.reconfigure ASconfig @09:33:46.04 AS.seek empty @09:33:47.08 Vacuum too high -- waiting up to 40s @09:33:47.56 Intake @48.2psia, vacuum from 20.0 to 24.0psi @09:33:47.57 SS.reconfigure forSampling @09:33:47.61 SS.seek 25.00ml @09:33:50.17 Vacuum too high -- waiting up to 40s @09:33:50.65 Intake @47.5psia, vacuum from 20.0 to 24.0psi @09:33:50.66 SS.reconfigure forSampling @09:33:50.70 SS.seek 25.00ml @09:33:53.52 Vacuum too high -- waiting up to 40s @09:33:54.01 Intake @47.7psia, vacuum from 20.0 to 24.0psi @09:33:54.03 SS.reconfigure forSampling @09:33:54.06 SS.seek 25.00ml @09:33:56.64 Vacuum too high -- waiting up to 40s @09:33:57.12 Intake @47.3psia, vacuum from 20.0 to 24.0psi @09:33:57.13 SS.reconfigure forSampling @09:33:57.17 SS.seek 25.00ml @09:34:00.17 Vacuum too high -- waiting up to 40s @09:34:00.86 Intake @48.0psia, vacuum from 20.0 to 24.0psi @09:34:00.87 SS.reconfigure forSampling @09:34:00.90 SS.seek 25.00ml @09:34:03.30 Vacuum too high -- waiting up to 40s @09:34:03.55 Parking Bleach in LysateSource line and SPEcolumn @09:34:03.56 ATV.dial :air3 @09:34:03.79 Intake @47.8psia, vacuum from 20.0 to 24.0psi @09:34:03.80 SS.reconfigure forSampling @09:34:03.84 SS.seek 25.00ml @09:34:04.14 AS.reconfigure ASslow1 @09:34:04.17 AS.seek 0.029ml @09:34:06.42 Vacuum too high -- waiting up to 40s @09:34:06.92 Intake @47.6psia, vacuum from 20.0 to 24.0psi @09:34:06.93 SS.reconfigure forSampling @09:34:06.96 SS.seek 25.00ml @09:34:09.33 Vacuum too high -- waiting up to 40s @09:34:09.81 Intake @47.3psia, vacuum from 20.0 to 24.0psi @09:34:09.82 SS.reconfigure forSampling @09:34:09.85 SS.seek 25.00ml @09:34:10.59 ATV.dial :ElutionWater @09:34:10.99 AS.seek 0.059ml @09:34:11.89 Vacuum too high -- waiting up to 40s @09:34:12.38 Intake @47.1psia, vacuum from 20.0 to 24.0psi @09:34:12.39 SS.reconfigure forSampling @09:34:12.42 SS.seek 25.00ml @09:34:14.64 Vacuum too high -- waiting up to 40s @09:34:15.11 Intake @47.3psia, vacuum from 20.0 to 24.0psi @09:34:15.12 SS.reconfigure forSampling @09:34:15.16 SS.seek 25.00ml @09:34:17.39 ATV.dial :air3 @09:34:17.61 Vacuum too high -- waiting up to 40s @09:34:17.78 AS.seek 0.069ml @09:34:18.16 Intake @48.4psia, vacuum from 20.0 to 24.0psi @09:34:18.18 SS.reconfigure forSampling @09:34:18.21 SS.seek 25.00ml @09:34:19.95 Vacuum too high -- waiting up to 40s @09:34:19.98 delay 2 seconds @09:34:21.10 Intake @47.7psia, vacuum from 20.0 to 24.0psi @09:34:21.12 SS.reconfigure forSampling @09:34:21.15 SS.seek 25.00ml @09:34:21.99 ATV.dial :bleach @09:34:22.49 AS.seek 0.709ml @09:34:24.14 Vacuum too high -- waiting up to 40s @09:34:24.63 Intake @47.7psia, vacuum from 20.0 to 24.0psi @09:34:24.65 SS.reconfigure forSampling @09:34:24.68 SS.seek 25.00ml @09:34:26.61 Vacuum too high -- waiting up to 40s @09:34:27.31 Intake @48.8psia, vacuum from 20.0 to 24.0psi @09:34:27.33 SS.reconfigure forSampling @09:34:27.36 SS.seek 25.00ml @09:34:29.45 Vacuum too high -- waiting up to 40s @09:34:29.93 Intake @48.0psia, vacuum from 20.0 to 24.0psi @09:34:29.95 SS.reconfigure forSampling @09:34:29.98 SS.seek 25.00ml @09:34:31.89 Vacuum too high -- waiting up to 40s @09:34:32.38 Intake @47.8psia, vacuum from 20.0 to 24.0psi @09:34:32.40 SS.reconfigure forSampling @09:34:32.43 SS.seek 25.00ml @09:34:34.33 Vacuum too high -- waiting up to 40s @09:34:34.80 Intake @47.2psia, vacuum from 20.0 to 24.0psi @09:34:34.81 SS.reconfigure forSampling @09:34:34.84 SS.seek 25.00ml @09:34:36.70 Vacuum too high -- waiting up to 40s @09:34:37.40 Intake @48.2psia, vacuum from 20.0 to 24.0psi @09:34:37.41 SS.reconfigure forSampling @09:34:37.44 SS.seek 25.00ml @09:34:39.67 Vacuum too high -- waiting up to 40s @09:34:40.34 Intake @48.4psia, vacuum from 20.0 to 24.0psi @09:34:40.36 SS.reconfigure forSampling @09:34:40.39 SS.seek 25.00ml @09:34:42.23 Vacuum too high -- waiting up to 40s @09:34:42.72 Intake @47.5psia, vacuum from 20.0 to 24.0psi @09:34:42.73 SS.reconfigure forSampling @09:34:42.77 SS.seek 25.00ml @09:34:44.73 Vacuum too high -- waiting up to 40s @09:34:45.42 Intake @47.2psia, vacuum from 20.0 to 24.0psi @09:34:45.43 SS.reconfigure forSampling @09:34:45.46 SS.seek 25.00ml @09:34:46.13 Stroke completed -- waiting for pressure to equalize ... @09:34:49.11 Intake.select :close @09:34:49.26 SSV.dial :exhaust @09:34:50.07 Exhaust.select :open @09:34:50.18 SS.reconfigure SSexhale @09:34:50.22 SS.seek 0.00ml @09:34:58.93 Exhaust.select :close @09:34:59.04 SSV.dial CBV @09:34:59.06 CBV.dial SSV @09:34:59.85 Allowing 185s for 25.0ml of remaining 800.0ml @09:34:59.86 Intake.select :open @09:35:02.00 Intake @48.3psia, vacuum from 20.0 to 24.0psi @09:35:02.01 SS.reconfigure forSampling @09:35:02.05 SS.seek 25.00ml @09:35:04.92 Vacuum too high -- waiting up to 40s @09:35:05.39 Intake @47.1psia, vacuum from 20.0 to 24.0psi @09:35:05.41 SS.reconfigure forSampling @09:35:05.44 SS.seek 25.00ml @09:35:07.58 Vacuum too high -- waiting up to 40s @09:35:08.29 Intake @47.8psia, vacuum from 20.0 to 24.0psi @09:35:08.30 SS.reconfigure forSampling @09:35:08.34 SS.seek 25.00ml @09:35:10.51 Vacuum too high -- waiting up to 40s @09:35:11.20 Intake @48.5psia, vacuum from 20.0 to 24.0psi @09:35:11.21 SS.reconfigure forSampling @09:35:11.24 SS.seek 25.00ml @09:35:12.98 Vacuum too high -- waiting up to 40s @09:35:13.92 Intake @47.7psia, vacuum from 20.0 to 24.0psi @09:35:13.93 SS.reconfigure forSampling @09:35:13.96 SS.seek 25.00ml @09:35:16.48 Vacuum too high -- waiting up to 40s @09:35:17.19 Intake @48.0psia, vacuum from 20.0 to 24.0psi @09:35:17.20 SS.reconfigure forSampling @09:35:17.24 SS.seek 25.00ml @09:35:19.01 Vacuum too high -- waiting up to 40s @09:35:19.50 Intake @47.5psia, vacuum from 20.0 to 24.0psi @09:35:19.51 SS.reconfigure forSampling @09:35:19.55 SS.seek 25.00ml @09:35:21.36 Vacuum too high -- waiting up to 40s @09:35:21.86 SS.seek 25.00ml @09:35:23.39 Vacuum too high -- waiting up to 40s @09:35:24.09 Intake @47.9psia, vacuum from 20.0 to 24.0psi @09:35:24.11 SS.reconfigure forSampling @09:35:24.14 SS.seek 25.00ml @09:35:25.98 Vacuum too high -- waiting up to 40s @09:35:26.69 Intake @48.9psia, vacuum from 20.0 to 24.0psi @09:35:26.71 SS.reconfigure forSampling @09:35:26.74 SS.seek 25.00ml @09:35:28.29 Vacuum too high -- waiting up to 40s @09:35:28.77 Intake @47.9psia, vacuum from 20.0 to 24.0psi @09:35:28.78 SS.reconfigure forSampling @09:35:28.82 SS.seek 25.00ml @09:35:30.48 Vacuum too high -- waiting up to 40s @09:35:31.17 Intake @47.7psia, vacuum from 20.0 to 24.0psi @09:35:31.18 SS.reconfigure forSampling @09:35:31.21 SS.seek 25.00ml @09:35:33.01 Vacuum too high -- waiting up to 40s @09:35:33.70 Intake @48.0psia, vacuum from 20.0 to 24.0psi @09:35:33.72 SS.reconfigure forSampling @09:35:33.75 SS.seek 25.00ml @09:35:35.54 Vacuum too high -- waiting up to 40s @09:35:36.25 Intake @48.2psia, vacuum from 20.0 to 24.0psi @09:35:36.26 SS.reconfigure forSampling @09:35:36.29 SS.seek 25.00ml @09:35:37.89 Vacuum too high -- waiting up to 40s @09:35:38.37 Intake @47.8psia, vacuum from 20.0 to 24.0psi @09:35:38.38 SS.reconfigure forSampling @09:35:38.42 SS.seek 25.00ml @09:35:39.92 Vacuum too high -- waiting up to 40s @09:35:40.61 Intake @48.0psia, vacuum from 20.0 to 24.0psi @09:35:40.62 SS.reconfigure forSampling @09:35:40.66 SS.seek 25.00ml @09:35:42.39 Vacuum too high -- waiting up to 40s @09:35:43.09 Intake @48.6psia, vacuum from 20.0 to 24.0psi @09:35:43.11 SS.reconfigure forSampling @09:35:43.14 SS.seek 25.00ml @09:35:44.67 Vacuum too high -- waiting up to 40s @09:35:45.15 Intake @47.9psia, vacuum from 20.0 to 24.0psi @09:35:45.16 SS.reconfigure forSampling @09:35:45.20 SS.seek 25.00ml @09:35:46.70 Vacuum too high -- waiting up to 40s @09:35:47.39 Intake @48.1psia, vacuum from 20.0 to 24.0psi @09:35:47.40 SS.reconfigure forSampling @09:35:47.43 SS.seek 25.00ml @09:35:49.11 Vacuum too high -- waiting up to 40s @09:35:49.79 Intake @48.7psia, vacuum from 20.0 to 24.0psi @09:35:49.80 SS.reconfigure forSampling @09:35:49.84 SS.seek 25.00ml @09:35:51.23 Vacuum too high -- waiting up to 40s @09:35:52.36 Intake @48.2psia, vacuum from 20.0 to 24.0psi @09:35:52.38 SS.reconfigure forSampling @09:35:52.41 SS.seek 25.00ml @09:35:54.48 Vacuum too high -- waiting up to 40s @09:35:54.97 Intake @47.5psia, vacuum from 20.0 to 24.0psi @09:35:54.99 SS.reconfigure forSampling @09:35:55.02 SS.seek 25.00ml @09:35:56.48 Vacuum too high -- waiting up to 40s @09:35:57.18 Intake @47.1psia, vacuum from 20.0 to 24.0psi @09:35:57.20 SS.reconfigure forSampling @09:35:57.23 SS.seek 25.00ml @09:35:58.92 Vacuum too high -- waiting up to 40s @09:35:59.63 Intake @48.2psia, vacuum from 20.0 to 24.0psi @09:35:59.64 SS.reconfigure forSampling @09:35:59.68 SS.seek 25.00ml @09:36:01.29 Vacuum too high -- waiting up to 40s @09:36:02.00 Intake @48.8psia, vacuum from 20.0 to 24.0psi @09:36:02.01 SS.reconfigure forSampling @09:36:02.05 SS.seek 25.00ml @09:36:03.54 Vacuum too high -- waiting up to 40s @09:36:04.03 Intake @47.7psia, vacuum from 20.0 to 24.0psi @09:36:04.04 SS.reconfigure forSampling @09:36:04.07 SS.seek 25.00ml @09:36:05.48 Vacuum too high -- waiting up to 40s @09:36:06.17 Intake @47.3psia, vacuum from 20.0 to 24.0psi @09:36:06.19 SS.reconfigure forSampling @09:36:06.22 SS.seek 25.00ml @09:36:07.76 Vacuum too high -- waiting up to 40s @09:36:08.47 Intake @47.5psia, vacuum from 20.0 to 24.0psi @09:36:08.48 SS.reconfigure forSampling @09:36:08.52 SS.seek 25.00ml @09:36:10.04 Vacuum too high -- waiting up to 40s @09:36:10.74 Intake @47.6psia, vacuum from 20.0 to 24.0psi @09:36:10.75 SS.reconfigure forSampling @09:36:10.79 SS.seek 25.00ml @09:36:12.35 Vacuum too high -- waiting up to 40s @09:36:13.05 Intake @47.9psia, vacuum from 20.0 to 24.0psi @09:36:13.06 SS.reconfigure forSampling @09:36:13.10 SS.seek 25.00ml @09:36:14.54 Vacuum too high -- waiting up to 40s @09:36:15.23 Intake @49.1psia, vacuum from 20.0 to 24.0psi @09:36:15.24 SS.reconfigure forSampling @09:36:15.28 SS.seek 25.00ml @09:36:16.51 Vacuum too high -- waiting up to 40s @09:36:16.98 Intake @48.2psia, vacuum from 20.0 to 24.0psi @09:36:16.99 SS.reconfigure forSampling @09:36:17.03 SS.seek 25.00ml @09:36:18.35 Vacuum too high -- waiting up to 40s @09:36:18.84 Intake @47.5psia, vacuum from 20.0 to 24.0psi @09:36:18.85 SS.reconfigure forSampling @09:36:18.89 SS.seek 25.00ml @09:36:20.17 Vacuum too high -- waiting up to 40s @09:36:20.87 Intake @47.6psia, vacuum from 20.0 to 24.0psi @09:36:20.88 SS.reconfigure forSampling @09:36:20.92 SS.seek 25.00ml @09:36:22.32 Vacuum too high -- waiting up to 40s @09:36:23.00 Intake @47.4psia, vacuum from 20.0 to 24.0psi @09:36:23.02 SS.reconfigure forSampling @09:36:23.05 SS.seek 25.00ml @09:36:24.51 Vacuum too high -- waiting up to 40s @09:36:25.19 Intake @47.0psia, vacuum from 20.0 to 24.0psi @09:36:25.20 SS.reconfigure forSampling @09:36:25.24 SS.seek 25.00ml @09:36:27.82 Vacuum too high -- waiting up to 40s @09:36:28.75 Intake @48.9psia, vacuum from 20.0 to 24.0psi @09:36:28.76 SS.reconfigure forSampling @09:36:28.80 SS.seek 25.00ml @09:36:30.20 Vacuum too high -- waiting up to 40s @09:36:30.69 Intake @47.3psia, vacuum from 20.0 to 24.0psi @09:36:30.71 SS.reconfigure forSampling @09:36:30.74 SS.seek 25.00ml @09:36:32.17 Vacuum too high -- waiting up to 40s @09:36:32.88 Intake @47.0psia, vacuum from 20.0 to 24.0psi @09:36:32.90 SS.reconfigure forSampling @09:36:32.93 SS.seek 25.00ml @09:36:33.29 delay 3 seconds @09:36:34.29 Vacuum too high -- waiting up to 40s @09:36:35.00 Intake @46.5psia, vacuum from 20.0 to 24.0psi @09:36:35.01 SS.reconfigure forSampling @09:36:35.05 SS.seek 25.00ml @09:36:36.30 ATV.dial :air3 @09:36:36.64 Vacuum too high -- waiting up to 40s @09:36:36.67 delay 3 seconds @09:36:37.33 Intake @46.4psia, vacuum from 20.0 to 24.0psi @09:36:37.34 SS.reconfigure forSampling @09:36:37.38 SS.seek 25.00ml @09:36:38.76 Vacuum too high -- waiting up to 40s @09:36:39.48 Intake @46.3psia, vacuum from 20.0 to 24.0psi @09:36:39.49 SS.reconfigure forSampling @09:36:39.53 SS.seek 25.00ml @09:36:39.73 AS.seek 0.734ml @09:36:40.98 Vacuum too high -- waiting up to 40s @09:36:41.88 Intake @47.6psia, vacuum from 20.0 to 24.0psi @09:36:41.90 SS.reconfigure forSampling @09:36:41.93 SS.seek 25.00ml @09:36:43.42 Vacuum too high -- waiting up to 40s @09:36:44.11 Intake @47.1psia, vacuum from 20.0 to 24.0psi @09:36:44.12 SS.reconfigure forSampling @09:36:44.15 SS.seek 25.00ml @09:36:45.01 delay 3 seconds @09:36:45.57 Vacuum too high -- waiting up to 40s @09:36:46.27 SS.seek 25.00ml @09:36:47.57 Vacuum too high -- waiting up to 40s @09:36:48.02 ATV.dial :bleach @09:36:48.26 Intake @46.8psia, vacuum from 20.0 to 24.0psi @09:36:48.27 SS.reconfigure forSampling @09:36:48.31 SS.seek 25.00ml @09:36:48.42 AS.seek 0.719ml @09:36:49.70 Vacuum too high -- waiting up to 40s @09:36:50.38 Intake @46.7psia, vacuum from 20.0 to 24.0psi @09:36:50.39 SS.reconfigure forSampling @09:36:50.43 SS.seek 25.00ml @09:36:51.70 Vacuum too high -- waiting up to 40s @09:36:51.74 delay 3 seconds @09:36:52.38 Intake @46.7psia, vacuum from 20.0 to 24.0psi @09:36:52.39 SS.reconfigure forSampling @09:36:52.42 SS.seek 25.00ml @09:36:53.76 Vacuum too high -- waiting up to 40s @09:36:54.67 Intake @47.8psia, vacuum from 20.0 to 24.0psi @09:36:54.68 SS.reconfigure forSampling @09:36:54.72 SS.seek 25.00ml @09:36:54.76 ATV.dial :AIV @09:36:55.89 AS.reconfigure ASconfig @09:36:55.92 AS.seek 0.015ml @09:36:56.07 Vacuum too high -- waiting up to 40s @09:36:57.04 Intake @47.1psia, vacuum from 20.0 to 24.0psi @09:36:57.05 SS.reconfigure forSampling @09:36:57.08 SS.seek 25.00ml @09:36:58.63 Vacuum too high -- waiting up to 40s @09:36:59.12 Intake @47.0psia, vacuum from 20.0 to 24.0psi @09:36:59.14 SS.reconfigure forSampling @09:36:59.17 SS.seek 25.00ml @09:37:00.45 Vacuum too high -- waiting up to 40s @09:37:01.15 Intake @46.7psia, vacuum from 20.0 to 24.0psi @09:37:01.17 SS.reconfigure forSampling @09:37:01.20 SS.seek 25.00ml @09:37:02.60 Vacuum too high -- waiting up to 40s @09:37:03.53 Intake @47.8psia, vacuum from 20.0 to 24.0psi @09:37:03.55 SS.reconfigure forSampling @09:37:03.58 SS.seek 25.00ml @09:37:04.91 Vacuum too high -- waiting up to 40s @09:37:05.61 Intake @47.1psia, vacuum from 20.0 to 24.0psi @09:37:05.63 SS.reconfigure forSampling @09:37:05.66 SS.seek 25.00ml @09:37:07.04 Vacuum too high -- waiting up to 40s @09:37:07.95 Intake @48.5psia, vacuum from 20.0 to 24.0psi @09:37:07.97 SS.reconfigure forSampling @09:37:08.00 SS.seek 25.00ml @09:37:09.26 Vacuum too high -- waiting up to 40s @09:37:09.95 Intake @49.0psia, vacuum from 20.0 to 24.0psi @09:37:09.97 SS.reconfigure forSampling @09:37:10.00 SS.seek 25.00ml @09:37:11.10 Vacuum too high -- waiting up to 40s @09:37:11.80 Intake @48.7psia, vacuum from 20.0 to 24.0psi @09:37:11.81 SS.reconfigure forSampling @09:37:11.84 SS.seek 25.00ml @09:37:13.07 Vacuum too high -- waiting up to 40s @09:37:13.77 Intake @48.3psia, vacuum from 20.0 to 24.0psi @09:37:13.78 SS.reconfigure forSampling @09:37:13.82 SS.seek 25.00ml @09:37:15.07 Vacuum too high -- waiting up to 40s @09:37:15.78 Intake @47.8psia, vacuum from 20.0 to 24.0psi @09:37:15.79 SS.reconfigure forSampling @09:37:15.82 SS.seek 25.00ml @09:37:17.07 Vacuum too high -- waiting up to 40s @09:37:17.74 Intake @47.5psia, vacuum from 20.0 to 24.0psi @09:37:17.76 SS.reconfigure forSampling @09:37:17.79 SS.seek 25.00ml @09:37:19.01 Vacuum too high -- waiting up to 40s @09:37:19.69 Intake @47.0psia, vacuum from 20.0 to 24.0psi @09:37:19.70 SS.reconfigure forSampling @09:37:19.74 SS.seek 25.00ml @09:37:21.10 Vacuum too high -- waiting up to 40s @09:37:21.79 Intake @46.9psia, vacuum from 20.0 to 24.0psi @09:37:21.80 SS.reconfigure forSampling @09:37:21.84 SS.seek 25.00ml @09:37:23.07 Vacuum too high -- waiting up to 40s @09:37:23.98 Intake @48.2psia, vacuum from 20.0 to 24.0psi @09:37:23.99 SS.reconfigure forSampling @09:37:24.03 SS.seek 25.00ml @09:37:25.23 Vacuum too high -- waiting up to 40s @09:37:25.91 Intake @47.9psia, vacuum from 20.0 to 24.0psi @09:37:25.92 SS.reconfigure forSampling @09:37:25.96 SS.seek 25.00ml @09:37:27.85 Vacuum too high -- waiting up to 40s @09:37:28.13 Intake @47.2psia, vacuum from 20.0 to 24.0psi @09:37:28.15 SS.reconfigure forSampling @09:37:28.18 SS.seek 25.00ml @09:37:29.60 Vacuum too high -- waiting up to 40s @09:37:30.31 Intake @47.6psia, vacuum from 20.0 to 24.0psi @09:37:30.33 SS.reconfigure forSampling @09:37:30.36 SS.seek 25.00ml @09:37:31.44 Vacuum too high -- waiting up to 40s @09:37:32.15 Intake @47.4psia, vacuum from 20.0 to 24.0psi @09:37:32.16 SS.reconfigure forSampling @09:37:32.20 SS.seek 25.00ml @09:37:33.44 Vacuum too high -- waiting up to 40s @09:37:34.14 Intake @47.0psia, vacuum from 20.0 to 24.0psi @09:37:34.16 SS.reconfigure forSampling @09:37:34.19 SS.seek 25.00ml @09:37:35.38 Vacuum too high -- waiting up to 40s @09:37:36.09 Intake @47.0psia, vacuum from 20.0 to 24.0psi @09:37:36.10 SS.reconfigure forSampling @09:37:36.14 SS.seek 25.00ml @09:37:37.22 Vacuum too high -- waiting up to 40s @09:37:37.26 ATV.dial :waste @09:37:37.91 Intake @46.3psia, vacuum from 20.0 to 24.0psi @09:37:37.92 SS.reconfigure forSampling @09:37:37.96 SS.seek 25.00ml @09:37:38.67 AS.seek empty @09:37:39.26 Vacuum too high -- waiting up to 40s @09:37:39.84 ATV.dial :air3 @09:37:40.01 Intake @46.8psia, vacuum from 20.0 to 24.0psi @09:37:40.02 SS.reconfigure forSampling @09:37:40.05 SS.seek 25.00ml @09:37:40.43 AS.reconfigure ASslow1 @09:37:40.46 AS.seek 0.009ml @09:37:41.23 Vacuum too high -- waiting up to 40s @09:37:41.94 Intake @47.2psia, vacuum from 20.0 to 24.0psi @09:37:41.95 SS.reconfigure forSampling @09:37:41.99 SS.seek 25.00ml @09:37:42.80 delay 2 seconds @09:37:43.16 Vacuum too high -- waiting up to 40s @09:37:43.86 Intake @47.1psia, vacuum from 20.0 to 24.0psi @09:37:43.87 SS.reconfigure forSampling @09:37:43.90 SS.seek 25.00ml @09:37:44.81 ATV.dial :bleach @09:37:45.04 Vacuum too high -- waiting up to 40s @09:37:45.20 AS.seek 0.579ml @09:37:45.79 Intake @46.7psia, vacuum from 20.0 to 24.0psi @09:37:45.80 SS.reconfigure forSampling @09:37:45.84 SS.seek 25.00ml @09:37:47.16 Vacuum too high -- waiting up to 40s @09:37:47.87 Intake @46.1psia, vacuum from 20.0 to 24.0psi @09:37:47.88 SS.reconfigure forSampling @09:37:47.92 SS.seek 25.00ml @09:37:49.13 Vacuum too high -- waiting up to 40s @09:37:49.82 Intake @46.2psia, vacuum from 20.0 to 24.0psi @09:37:49.84 SS.reconfigure forSampling @09:37:49.87 SS.seek 25.00ml @09:37:51.01 Vacuum too high -- waiting up to 40s @09:37:51.71 Intake @45.8psia, vacuum from 20.0 to 24.0psi @09:37:51.72 SS.reconfigure forSampling @09:37:51.76 SS.seek 25.00ml @09:37:53.04 Vacuum too high -- waiting up to 40s @09:37:53.73 Intake @46.0psia, vacuum from 20.0 to 24.0psi @09:37:53.74 SS.reconfigure forSampling @09:37:53.78 SS.seek 25.00ml @09:37:56.38 Vacuum too high -- waiting up to 40s @09:37:57.31 Intake @47.4psia, vacuum from 20.0 to 24.0psi @09:37:57.32 SS.reconfigure forSampling @09:37:57.36 SS.seek 25.00ml @09:37:58.47 Vacuum too high -- waiting up to 40s @09:37:59.17 Intake @46.8psia, vacuum from 20.0 to 24.0psi @09:37:59.19 SS.reconfigure forSampling @09:37:59.22 SS.seek 25.00ml @09:38:00.44 Vacuum too high -- waiting up to 40s @09:38:01.15 Intake @46.4psia, vacuum from 20.0 to 24.0psi @09:38:01.16 SS.reconfigure forSampling @09:38:01.20 SS.seek 25.00ml @09:38:02.41 Vacuum too high -- waiting up to 40s @09:38:03.13 Intake @46.4psia, vacuum from 20.0 to 24.0psi @09:38:03.14 SS.reconfigure forSampling @09:38:03.17 SS.seek 25.00ml @09:38:03.78 Stroke completed -- waiting for pressure to equalize ... @09:38:04.86 SS.reconfigure SSconfig @09:38:04.90 SS.hold @09:38:04.99 Intake.select :close @09:38:05.15 ShallowSampler::Clogged in WCR -- progress too slow after 225.0ml @09:38:05.16 SSV.dial :exhaust @09:38:05.95 Exhaust.select :open @09:38:06.05 SS.reconfigure SSexhale @09:38:06.09 SS.seek 0.00ml @09:38:15.25 Exhaust.select :close @09:38:15.37 Sampled 225.0ml @09:38:15.38 collectSample: Complete @09:38:15.39 Drying puck before fixing for 04:00.00 @09:38:15.40 CBV.dial :waste2b @09:38:16.19 delay 1:00 @09:38:18.36 Sleepy.power :sampler,:OFF @09:39:16.19 CSV.dial :air @09:39:16.64 CS.reconfigure CSfast @09:39:16.67 CS.seek 10.000ml @09:39:40.74 CBV.dial between CSV and waste2b @09:39:41.05 Pumping wcr puck from tube 3 up to 55psi... @09:39:41.09 CBV.dial SSV @09:39:41.64 delay 3 seconds @09:39:42.53 CBV.dial between CSV and waste2b @09:39:43.48 CSV.dial between air and CTV @09:39:43.88 CS.seek 6.781ml @09:39:44.65 ATV.dial :air3 @09:39:44.99 delay 3 seconds @09:39:48.05 AS.seek 0.604ml @09:39:52.09 CTV.dial CSV,avoiding => air @09:39:52.11 CSV.dial CTV @09:39:52.84 CS.reconfigure CSpressurize @09:39:52.87 CS.seek 0.000ml @09:39:53.39 delay 3 seconds @09:39:56.40 ATV.dial :bleach @09:39:56.88 AS.seek 0.589ml @09:40:00.17 delay 3 seconds @09:40:03.18 ATV.dial :ASV @09:40:04.16 ASV.dial :wasteR @09:40:04.98 AS.seek 0.000ml @09:40:08.14 CBV.dial :waste2b @09:40:08.46 CS.reconfigure CSconfig @09:40:08.50 CS.seek empty @09:40:11.58 delay 1:00 @09:41:11.59 CTV.dial :air,avoiding => intake @09:41:12.87 Fix #1 of 2 (with rnal) for 02:00.00 @09:41:12.88 CS Pulling 2ml of rnal CSV.dial CSR @09:41:13.32 CSR.select :rnal @09:41:13.41 CS.seek 2.000ml @09:41:19.33 delay 5 seconds @09:41:24.34 CSR.select :air @09:41:24.43 CS.reconfigure CSfast @09:41:24.47 CS.seek 10.000ml @09:41:43.70 CBV.dial between CSV and waste2b @09:41:44.03 Pumping wcr puck from tube 3 up to 55psi... @09:41:44.07 CBV.dial SSV @09:41:45.51 CBV.dial between CSV and waste2b @09:41:46.46 CSV.dial between air and CTV @09:41:47.12 CS.seek 6.664ml @09:41:55.56 CTV.dial CSV,avoiding => air @09:41:55.58 CSV.dial CTV @09:41:56.95 CS.reconfigure CSpressurize @09:41:56.98 CS.seek 0.000ml @09:42:05.51 delay 1:00 @09:42:14.10 delay 2:00 @09:43:05.52 AFV.select :carrierSolution @09:43:05.61 delay 2 seconds @09:43:07.62 AS.reconfigure ASconfig @09:43:07.65 AS.seek 1.000ml @09:44:06.25 delay 1 second @09:44:07.26 AFV.select :holdingCoil @09:44:07.35 delay 1 second @09:44:08.36 ATV.dial :ASV @09:44:08.48 ASV.dial :wasteR @09:44:08.49 AS.reconfigure ASslow1 @09:44:08.52 AS.seek 0.000ml @09:44:14.11 CBV.dial :waste2b @09:44:14.44 CS.reconfigure CSconfig @09:44:14.47 CS.seek empty @09:44:16.01 delay 1:00 @09:45:16.02 CTV.dial :air,avoiding => intake @09:45:17.30 Fix #2 of 2 (with rnal) for 02:00.00 @09:45:17.31 CS Pulling 2ml of rnal CSV.dial CSR @09:45:17.82 CSR.select :rnal @09:45:17.92 CS.seek 2.000ml @09:45:23.82 delay 5 seconds @09:45:28.83 CSR.select :air @09:45:28.92 CS.reconfigure CSfast @09:45:28.96 CS.seek 10.000ml @09:45:48.16 CBV.dial between CSV and waste2b @09:45:48.48 Pumping wcr puck from tube 3 up to 55psi... @09:45:48.52 CBV.dial SSV @09:45:49.95 CBV.dial between CSV and waste2b @09:45:50.90 CSV.dial between air and CTV @09:45:51.56 CS.seek 6.550ml @09:46:00.30 CTV.dial CSV,avoiding => air @09:46:00.32 CSV.dial CTV @09:46:01.64 CS.reconfigure CSpressurize @09:46:01.67 CS.seek 0.000ml @09:46:17.65 delay 2:00 @09:47:32.77 delay 20 seconds @09:47:52.78 ATV.dial :air3 @09:47:53.91 AS.seek 0.590ml @09:48:17.66 CBV.dial :waste2b @09:48:17.98 CS.reconfigure CSconfig @09:48:18.01 CS.seek empty @09:48:19.75 delay 1:00 @09:49:19.76 CTV.dial :air,avoiding => intake @09:49:21.08 Storing wcr puck in Collection Clamp to tube 2 @09:49:21.21 SC.reconfigure SCtight @09:49:21.24 SC.seek 2 @09:49:23.48 SC.reconfigure SCloose @09:49:23.67 Elbow.reconfigure TightElbow @09:49:23.70 Elbow.seek 2 @09:49:25.30 Forearm.reconfigure TightForearm @09:49:25.33 Forearm.seek 2 + 800 counts @09:49:26.47 SE.reconfigure SEpick @09:49:26.52 SE.seek beamBreak @09:49:29.42 SE.configure SEconfig @09:49:29.54 Elbow.reconfigure LimpElbow @09:49:29.57 Forearm.reconfigure LooseForearm @09:49:29.61 Forearm.seek 2 @09:49:29.84 Elbow.hold @09:49:29.89 Elbow.reconfigure TightElbow @09:49:29.97 SE.seek 0.04 Pucks below Top Plate @09:49:30.26 Forearm.coast @09:49:30.30 Forearm.seek 2 + 343 counts @09:49:30.53 Forearm.reconfigure TightForearm @09:49:30.57 Forearm.seek 2 + 800 counts @09:49:30.80 SE.seek 1.04 Pucks below Top Plate @09:49:31.34 Forearm.reconfigure LooseForearm @09:49:31.37 Forearm.seek retract @09:49:32.33 Elbow.seek CC @09:49:33.87 Elbow.reconfigure LooseElbow @09:49:33.95 Forearm.seek CC @09:49:34.91 CC.seek sealed @09:49:35.81 Hand.grip @09:49:36.58 CC.seek open @09:49:40.56 Forearm.seek retract @09:49:41.55 Elbow.reconfigure TightElbow @09:49:41.58 Elbow.seek 2 @09:49:43.19 Elbow.reconfigure LimpElbow @09:49:43.23 Forearm.seek 2 @09:49:44.25 Elbow.hold @09:49:44.31 Elbow.reconfigure TightElbow @09:49:44.35 Hand.release @09:49:45.16 Forearm.coast @09:49:45.19 Forearm.seek 2 + 318 counts @09:49:45.44 Forearm.reconfigure TightForearm @09:49:45.47 Forearm.seek 2 + 800 counts @09:49:45.80 SE.seek down @09:49:46.91 Forearm.reconfigure LooseForearm @09:49:46.94 Forearm.seek retract @09:49:48.42 SE.coast @09:49:48.45 SC.coast @09:49:48.48 Starting finalPurge @09:49:48.49 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @09:49:48.63 Elbow.seek FlushPuck::Garage @09:49:50.33 Elbow.reconfigure LooseElbow @09:49:50.41 Forearm.seek garage @09:49:51.43 Hand.grip @09:49:52.30 Forearm.seek retract @09:49:53.32 Elbow.reconfigure TightElbow @09:49:53.35 Elbow.seek CC @09:49:54.50 delay 3 seconds @09:49:56.16
Elbow.reconfigure LooseElbow @09:49:56.25 Forearm.reconfigure TightForearm @09:49:56.28 Forearm.seek CC @09:49:57.33 CC.seek closed @09:49:57.51 ATV.dial :ASV @09:49:58.59 ASV.dial :wasteR @09:49:58.68 AS.seek 0.090ml @09:50:01.11
Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.99 @09:50:01.16 Hand.release @09:50:02.00 Forearm.reconfigure LooseForearm @09:50:02.03 Forearm.seek retract @09:50:02.99 Flushing the Collection Valve::Manifold @09:50:03.10 CS Pulling 5ml of flush CSV.dial CSR @09:50:03.53 CSR.select :flush @09:50:03.63 CS.seek 5.000ml @09:50:17.12 delay 5 seconds @09:50:22.13 CSR.select :air @09:50:22.27 CS.reconfigure CSfast @09:50:22.30 CS.seek 7.499ml @09:50:28.99 CSV.dial :waste @09:50:30.27 CS.reconfigure CSconfig @09:50:30.30 CS.seek 0.000ml @09:50:50.19 Flushing lower manifold's waste1 port @09:50:50.28 CSV.dial :flush @09:50:50.71 CS.reconfigure CSfast @09:50:50.74 CS.seek 5.000ml @09:51:03.06 delay 5 seconds @09:51:08.07 CSV.dial :air @09:51:08.53 CS.seek 7.000ml @09:51:14.13 CSV.dial CTV @09:51:14.15
CTV.dial CSV @09:51:15.47 CBV.dial :waste1 @09:51:16.21 CS.seek 0.000ml @09:51:32.88 Flushing lower manifold's waste2b port @09:51:33.61 CSV.dial :flush @09:51:34.42 CS.seek 5.000ml @09:51:40.90 delay 30 seconds @09:51:46.69
delay 5 seconds @09:51:51.70 CSV.dial :air @09:51:52.27 CS.seek 7.000ml @09:51:57.75 CSV.dial CTV @09:51:57.77
CTV.dial CSV @09:51:58.21 CBV.dial :waste2b @09:51:58.95 CS.seek 0.000ml @09:52:10.91 ATV.dial :extSrc @09:52:11.43 delay 3 seconds @09:52:14.44 ATV.dial :ElutionWater @09:52:15.48 AS.seek 0.120ml @09:52:15.53
Flushing upper manifold @09:52:15.62 CSV.dial :flush @09:52:16.43 CS.seek 5.000ml @09:52:21.86 ATV.dial :air3 @09:52:22.34 AS.seek 0.130ml @09:52:24.66 delay 2 seconds @09:52:26.67 ATV.dial :bleach @09:52:27.15 AS.seek 0.230ml @09:52:28.78
delay 5 seconds @09:52:33.79 CSV.dial :air @09:52:34.75 CS.seek 7.000ml @09:52:40.15 CSV.dial CBV @09:52:40.17
CBV.dial CSV @09:52:41.30 CTV.dial :air @09:52:42.60 CS.seek 0.000ml @09:52:47.79 delay 3 seconds @09:52:50.80 ATV.dial :air3 @09:52:51.13 delay 3 seconds @09:52:54.19 AS.seek 0.255ml @09:52:59.31
delay 7 seconds @09:52:59.54 delay 3 seconds @09:53:02.55 ATV.dial :bleach @09:53:02.94 AS.seek 0.240ml @09:53:06.25 delay 3 seconds @09:53:06.32
CS.seek 3.000ml @09:53:09.26 ATV.dial :ASV @09:53:10.13 ASV.dial :SPEdelivery @09:53:10.44 AFV.select :carrierSolution @09:53:10.54 delay 2 seconds @09:53:12.55 AS.reconfigure ASconfig @09:53:12.59 AS.seek 0.500ml @09:53:14.03
CSV.dial :waste @09:53:14.54 CS.seek 0.000ml @09:53:22.23 CSV.dial :flush @09:53:22.66 CS.seek 3.000ml @09:53:28.00 delay 1 second @09:53:29.01 AFV.select :holdingCoil @09:53:29.15 AS.reconfigure ASslow1 @09:53:29.18 AS.seek 0.170ml @09:53:30.40
delay 5 seconds @09:53:35.41 CSV.dial :air @09:53:35.89 CS.seek 5.000ml @09:53:41.28 CSV.dial :air @09:53:41.30 CS.seek 0.000ml @09:53:53.40 delay 7 seconds @09:54:00.41 CS.seek 3.000ml @09:54:08.06 CSV.dial :waste @09:54:08.85 CS.seek 0.000ml @09:54:16.62 Disinfecting external Intake with mfbkill @09:54:16.71 CSV.dial CSR @09:54:18.06 CSR.select :mfbkill @09:54:18.16 CS.seek 8.000ml @09:54:36.77 SPE.hold 2:46:40,95.0C @09:54:36.80 delay 3:00 @09:54:37.30
delay 5 seconds @09:54:42.32 CSR.select :air @09:54:42.41 CS.seek 10.000ml @09:54:48.09 CSV.dial CBV @09:54:48.14
CBV.dial CSV @09:54:49.16 CTV.dial :intake @09:54:49.93 Intake.select :open @09:54:50.02 CS.seek 2.000ml @09:55:09.83 Intake.select :close @09:55:09.92 CTV.dial :air @09:55:10.69 CS.reconfigure CSconfig @09:55:10.73 CS.seek 0.000ml @09:55:16.58 Flushing lower manifold's waste1 port @09:55:16.59 CS Pulling 4ml of mfbkill @09:55:16.60 CSV.dial CSR @09:55:17.69 CSR.select :mfbkill @09:55:17.79 CS.seek 4.000ml @09:55:28.80 delay 5 seconds @09:55:33.81 CSR.select :air @09:55:33.90 CS.reconfigure CSfast @09:55:33.94 CS.seek 10.000ml @09:55:48.96 CSV.dial CTV @09:55:48.99
CTV.dial CSV @09:55:50.35 CBV.dial :waste1 @09:55:50.81 CS.seek 0.000ml @09:56:14.83 Flushing lower manifold's waste2b port @09:56:14.84 CS Pulling 4ml of mfbkill @09:56:14.85 CSV.dial CSR @09:56:15.94 CSR.select :mfbkill @09:56:16.04 CS.reconfigure CSconfig @09:56:16.08 CS.seek 4.000ml @09:56:27.11 delay 5 seconds @09:56:32.12 CSR.select :air @09:56:32.21 CS.reconfigure CSfast @09:56:32.25 CS.seek 10.000ml @09:56:47.43 CSV.dial CTV @09:56:47.45
CTV.dial CSV @09:56:47.98 CBV.dial :waste2b @09:56:48.75 CS.seek 0.000ml @09:57:12.89 Killing upper manifold @09:57:12.90 CS Pulling 4ml of mfbkill @09:57:12.91 CSV.dial CSR @09:57:13.48 CSR.select :mfbkill @09:57:13.58 CS.reconfigure CSconfig @09:57:13.62 CS.seek 4.000ml @09:57:24.58 delay 5 seconds @09:57:29.59 CSR.select :air @09:57:29.68 CS.reconfigure CSfast @09:57:29.72 CS.seek 10.000ml @09:57:36.81 AFV.select :carrierSolution @09:57:36.90 delay 2 seconds @09:57:38.91 AS.reconfigure ASconfig @09:57:38.94 AS.seek 1.000ml @09:57:44.70 CSV.dial CBV @09:57:44.72
CBV.dial CSV @09:57:45.98 CTV.dial :air @09:57:47.31 CS.seek 0.000ml @09:58:11.45 delay 7 seconds @09:58:18.46 CS.seek 3.000ml @09:58:26.36 CSV.dial :waste @09:58:26.88 CS.seek 0.000ml @09:58:27.60 delay 1 second @09:58:28.61 AFV.select :holdingCoil @09:58:28.70 delay 1 second @09:58:29.71 ASV.dial :wasteD @09:58:30.74 AS.seek 0.000ml @09:58:34.61
Flushing upper manifold @09:58:34.70 CSV.dial :flush @09:58:35.15 CS.seek 5.000ml @09:58:47.51 delay 5 seconds @09:58:52.52 CSV.dial :air @09:58:53.00 CS.seek 6.999ml @09:58:58.42 CSV.dial CBV @09:58:58.44
CBV.dial CSV @09:58:59.53 CTV.dial :air @09:58:59.55 CS.seek 0.000ml @09:59:16.32 delay 7 seconds @09:59:23.33 CS.seek 3.000ml @09:59:29.35 Priming Eluate @09:59:29.36 AFV.select :carrierSolution @09:59:29.45 delay 2 seconds @09:59:31.14
CSV.dial :waste @09:59:31.46 AS.seek 0.500ml @09:59:31.66
CS.seek 0.000ml @09:59:39.46 CSV.dial :flush @09:59:39.91 CS.seek 3.000ml @09:59:47.60 delay 5 seconds @09:59:52.61 CSV.dial :air @09:59:53.07 CS.seek 5.000ml @09:59:58.54 CSV.dial :air @09:59:58.56 CS.seek 0.000ml @10:00:00.88 delay 1 second @10:00:01.89 AFV.select :holdingCoil @10:00:01.98 ATV.dial ASV @10:00:01.99 ASV.dial :SPErecovery @10:00:02.65 Swish #1 of 2 @10:00:02.71 AS.reconfigure ASslow0 @10:00:02.74 AS.seek 0.600ml @10:00:10.73
delay 7 seconds @10:00:13.29 AS.seek 0.500ml @10:00:17.74
CS.seek 3.000ml @10:00:23.77 Swish #2 of 2 @10:00:23.83 AS.seek 0.600ml @10:00:25.48
CSV.dial :waste @10:00:26.28 CS.seek 0.000ml @10:00:33.98 Flushing lower manifold's waste1 port @10:00:34.07 CSV.dial :flush @10:00:34.45 AS.seek 0.500ml @10:00:34.60
CS.seek 5.000ml @10:00:44.96 delay 3 seconds @10:00:46.98
delay 5 seconds @10:00:48.02 AS.reconfigure ASslow1 @10:00:48.06 AS.seek 0.676ml @10:00:51.99
CSV.dial :air @10:00:52.46 CS.seek 7.000ml @10:00:57.85 CSV.dial CTV @10:00:57.87
CTV.dial CSV @10:00:59.21 CBV.dial :waste1 @10:00:59.67 CS.seek 0.000ml @10:01:16.44 Flushing lower manifold's waste2b port @10:01:16.53 CSV.dial :flush @10:01:17.34 CS.seek 5.000ml @10:01:24.24 delay 3 seconds @10:01:27.25 Eluate primed @10:01:27.26 ATV.dial :waste2 @10:01:27.78 AS.reconfigure ASnorm @10:01:27.81 AS.seek 0.000ml @10:01:29.66
delay 5 seconds @10:01:34.67 CSV.dial :air @10:01:35.63 CS.seek 7.000ml @10:01:41.04 CSV.dial CTV @10:01:41.06
CTV.dial CSV @10:01:41.51 CBV.dial :waste2b @10:01:42.26 CS.seek 0.000ml @10:01:58.88 Disinfecting external Intake with kill @10:01:58.97 CSV.dial CSR @10:01:59.39 CSR.select :kill @10:01:59.49 CS.seek 8.000ml @10:02:03.27 SPE.coast @10:02:03.35 ASV.dial :SPErecovery @10:02:03.37 Setting up PCR for HF183em -- writing /var/log/esp/HF183em14mar1110h222ml.pcr @10:02:03.48 Building PCR Slug from ASV with MM2 mix, PP4 primer @10:02:03.49 Priming with carrier @10:02:03.50 ATV.dial ARV @10:02:04.21 ARV.dial :carrier @10:02:04.51 AS.seek 0.500ml @10:02:18.44
delay 5 seconds @10:02:23.45 CSR.select :air @10:02:23.54 CS.seek 10.000ml @10:02:28.94 CSV.dial CBV @10:02:28.96
CBV.dial CSV @10:02:29.99 CTV.dial :intake @10:02:30.39 AFV.select :carrierSolution @10:02:30.49 delay 2 seconds @10:02:30.72
Intake.select :open @10:02:30.83 CS.seek 2.000ml @10:02:32.50 AS.reconfigure ASconfig @10:02:32.54 AS.seek 1.000ml @10:02:49.78
Intake.select :close @10:02:49.87 CTV.dial :air @10:02:50.60 CS.reconfigure CSconfig @10:02:50.64 CS.seek 0.000ml @10:02:56.47 CSV.dial CSR @10:02:57.53 CSR.select :flush @10:02:57.63 CS.seek 5.000ml @10:03:01.95 delay 1 second @10:03:02.96 AFV.select :holdingCoil @10:03:03.06 delay 1 second @10:03:04.07 ATV.dial :waste @10:03:05.32 AS.reconfigure ASnorm @10:03:05.35 AS.seek empty @10:03:11.06
delay 5 seconds @10:03:16.07 CSR.select :air @10:03:16.17 CS.seek 10.000ml @10:03:29.59 CSV.dial CBV @10:03:30.65 CS.seek 0.000ml @10:03:56.81 Garaging FlushPuck in Collection Clamp @10:03:56.87 AFV.select :carrierSolution @10:03:56.91
Elbow.reconfigure TightElbow @10:03:56.95 Elbow.seek CC @10:03:56.98 delay 2 seconds @10:03:57.13
Elbow.reconfigure LooseElbow @10:03:57.22 Forearm.seek CC @10:03:58.17 CC.seek sealed @10:03:58.99 AS.reconfigure ASconfig @10:03:59.03 AS.seek 0.500ml @10:03:59.12
Hand.grip @10:03:59.81 CC.seek open @10:04:03.13 Forearm.seek retract @10:04:04.12 Elbow.reconfigure TightElbow @10:04:04.16 Elbow.seek garage @10:04:06.96 Elbow.reconfigure LooseElbow @10:04:07.05 Forearm.reconfigure TightForearm @10:04:07.08 Forearm.seek garage @10:04:08.23 Hand.release @10:04:08.97 Forearm.reconfigure LooseForearm @10:04:09.00 Forearm.seek retract @10:04:28.51 delay 1 second @10:04:29.52 AFV.select :holdingCoil @10:04:29.61 Building Slug @10:04:29.62 ATV.dial :ARV @10:04:30.97 delay 2 seconds @10:04:32.98 ARV.dial :air1 @10:04:33.33 delay 2 seconds @10:04:35.39 AS.reconfigure ASslow2 @10:04:35.42 AS.seek 0.506ml @10:04:41.86 delay 2 seconds @10:04:43.87 ARV.dial :MM2 @10:04:45.20 delay 2 seconds @10:04:47.21 Adding MM2 @10:04:47.26 AS.seek 0.524ml @10:05:05.82 delay 25 seconds @10:05:30.83 ARV.dial :PP4 @10:05:31.15 Adding PP4 @10:05:31.16 delay 2 seconds @10:05:33.22 AS.seek 0.530ml @10:05:39.46 delay 10 seconds @10:05:49.47 ARV.dial :air3 @10:05:49.79 adding ARV air @10:05:49.80 delay 2 seconds @10:05:51.86 AS.seek 0.536ml @10:05:58.18 delay 5 seconds @10:06:03.19 ARV.dial :carrier @10:06:04.81 delay 7 seconds @10:06:11.87 AS.seek 0.594ml @10:07:11.21 delay 5 seconds @10:07:16.24 ATV.dial :ASV @10:07:16.57 delay 2 seconds @10:07:18.63 AS.seek 0.600ml @10:07:24.96 delay 4 seconds @10:07:28.97 ATV.dial ARV @10:07:29.30 delay 2 seconds @10:07:31.31 ARV.dial :carrier @10:07:31.32 delay 2 seconds @10:07:33.37 AS.seek 0.650ml @10:08:24.77 delay 4 seconds @10:08:28.78 ATV.dial PCR @10:08:29.30 ASV.dial :waste1 @10:08:29.98 ARV.dial :air1 @10:08:30.42 Positioning Slug by 0.324ml @10:08:30.47 AS.seek 0.326ml @10:14:00.96 delay 5 seconds @10:14:05.97 ATV.dial ARV @10:14:06.58 ASV.dial :PCRlock @10:14:07.02 delay 5 seconds @10:14:12.03 Running HF183em ProfileEM60C2 thermocycling @10:14:12.04 PCR.program ProfileEM60C2 @10:14:12.15 ProfileEM60C2 should complete in < 2:11:38 @10:14:12.16 PCR.run 2:49:32.5 @12:09:29.48 PCR.off @12:09:30.01 PCR for HF183em completed -- closed /var/log/esp/HF183em14mar1110h222ml.pcr @12:09:30.06 Cleaning PCR @12:09:30.07 AFV.select :carrierSolution @12:09:30.16 delay 2 seconds @12:09:32.17 AS.reconfigure ASconfig @12:09:32.20 AS.seek 0.500ml @12:09:42.66 delay 1 second @12:09:43.67 AFV.select :holdingCoil @12:09:43.76 ASV.dial :waste1 @12:09:44.21 ATV.dial :air3 @12:09:45.14 AS.reconfigure ASslow1 @12:09:45.18 AS.seek 0.510ml @12:09:47.49 delay 2 seconds @12:09:49.50 ATV.dial :bleach @12:09:49.99 AS.seek 0.810ml @12:10:51.42 delay 3 seconds @12:10:54.43 ATV.dial :air3 @12:10:54.75 delay 3 seconds @12:10:57.81 AS.seek 0.835ml @12:11:03.16 delay 3 seconds @12:11:06.17 ATV.dial :bleach @12:11:06.57 AS.seek 0.820ml @12:11:09.86 delay 3 seconds @12:11:12.87 ARV.dial :waste @12:11:13.44 delay 3 seconds @12:11:17.07 ATV.dial :ARV @12:11:17.79 AS.reconfigure ASconfig @12:11:17.83 AS.seek 0.470ml @12:11:38.53 AFV.select :carrierSolution @12:11:38.62 delay 2 seconds @12:11:40.63 AS.seek 1.000ml @12:12:11.84 delay 1 second @12:12:12.85 AFV.select :holdingCoil @12:12:12.94 delay 1 second @12:12:13.95 AS.reconfigure ASfast @12:12:13.99 AS.seek 0.000ml @12:12:41.73 AFV.select :carrierSolution @12:12:41.83 delay 2 seconds @12:12:43.84 AS.reconfigure ASconfig @12:12:43.87 AS.seek 1.000ml @12:13:42.42 delay 1 second @12:13:43.43 AFV.select :holdingCoil @12:13:43.65 delay 1 second @12:13:44.66 AS.reconfigure ASfast @12:13:44.69 AS.seek 0.000ml @12:14:12.43 AFV.select :carrierSolution @12:14:12.53 delay 2 seconds @12:14:14.54 AS.reconfigure ASconfig @12:14:14.57 AS.seek 1.000ml @12:15:13.20 delay 1 second @12:15:14.21 AFV.select :holdingCoil @12:15:14.30 delay 1 second @12:15:15.31 AS.reconfigure ASfast @12:15:15.34 AS.seek 0.000ml @12:15:43.07 ARV.dial between carrier and air1 @12:15:43.48 ATV.dial :air3 @12:15:44.42 AS.reconfigure ASslow1 @12:15:44.46 AS.seek 0.010ml @12:15:46.79 delay 2 seconds @12:15:48.80 ATV.dial :bleach @12:15:49.20 AS.seek 0.810ml @12:18:32.58 delay 3 seconds @12:18:35.59 ATV.dial :air3 @12:18:35.92 delay 3 seconds @12:18:38.98 AS.seek 0.835ml @12:18:44.31 delay 3 seconds @12:18:47.32 ATV.dial :bleach @12:18:47.71 AS.seek 0.820ml @12:18:51.03 delay 3 seconds @12:18:54.04 ATV.dial PCR @12:18:54.36 AS.reconfigure ASconfig @12:18:54.39 AS.seek empty @12:19:42.50 PCR.program :bleachProfile @12:19:42.55 PCR program should complete in < 2:45 @12:19:42.56 PCR.run 10:00 @12:22:43.77 PCR.off @12:22:43.80 AFV.select :carrierSolution @12:22:43.89 delay 2 seconds @12:22:45.90 AS.seek 1.000ml @12:23:44.54 delay 1 second @12:23:45.55 AFV.select :holdingCoil @12:23:45.64 delay 1 second @12:23:46.65 AS.reconfigure ASfast @12:23:46.68 AS.seek 0.000ml @12:24:14.44 delay 3 seconds @12:24:17.45 AFV.select :carrierSolution @12:24:17.54 delay 2 seconds @12:24:19.55 AS.reconfigure ASconfig @12:24:19.58 AS.seek 1.000ml @12:25:18.19 delay 1 second @12:25:19.20 AFV.select :holdingCoil @12:25:19.29 delay 1 second @12:25:20.30 AS.reconfigure ASfast @12:25:20.33 AS.seek 0.000ml @12:25:48.06 delay 3 seconds @12:25:51.07 AFV.select :carrierSolution @12:25:51.16 delay 2 seconds @12:25:53.17 AS.reconfigure ASconfig @12:25:53.20 AS.seek 1.000ml @12:26:51.82 delay 1 second @12:26:52.83 AFV.select :holdingCoil @12:26:52.92 delay 1 second @12:26:53.93 AS.reconfigure ASfast @12:26:53.96 AS.seek 0.000ml @12:27:21.68 delay 3 seconds @12:27:24.69 AFV.select :carrierSolution @12:27:24.78 delay 2 seconds @12:27:26.79 AS.reconfigure ASconfig @12:27:26.82 AS.seek 1.000ml @12:28:25.42 delay 1 second @12:28:26.43 AFV.select :holdingCoil @12:28:26.52 delay 1 second @12:28:27.53 AS.reconfigure ASfast @12:28:27.56 AS.seek 0.000ml @12:28:55.32 delay 3 seconds @12:28:58.33 AFV.select :carrierSolution @12:28:58.42 delay 2 seconds @12:29:00.43 AS.reconfigure ASconfig @12:29:00.46 AS.seek 1.000ml @12:29:59.05 delay 1 second @12:30:00.06 AFV.select :holdingCoil @12:30:00.15 delay 1 second @12:30:01.16 AS.reconfigure ASnorm @12:30:01.19 AS.seek 0.000ml @12:30:52.66 Setting up PCR for ENTap -- writing /var/log/esp/ENTap14mar1112h222ml.pcr @12:30:52.67 Building PCR Slug from ASV with MM1 mix, PP1 primer @12:30:52.68 Priming with carrier @12:30:52.69 ATV.dial ARV @12:30:53.21 ARV.dial :carrier @12:30:53.49 AS.seek 0.500ml @12:31:19.35 AFV.select :carrierSolution @12:31:19.44 delay 2 seconds @12:31:21.45 AS.reconfigure ASconfig @12:31:21.48 AS.seek 1.000ml @12:31:50.92 delay 1 second @12:31:51.93 AFV.select :holdingCoil @12:31:52.02 delay 1 second @12:31:53.03 ATV.dial :waste @12:31:54.29 AS.reconfigure ASnorm @12:31:54.32 AS.seek empty @12:32:45.77 AFV.select :carrierSolution @12:32:45.86 delay 2 seconds @12:32:47.87 AS.reconfigure ASconfig @12:32:47.90 AS.seek 0.500ml @12:33:17.37 delay 1 second @12:33:18.38 AFV.select :holdingCoil @12:33:18.47 Building Slug @12:33:18.48 ATV.dial :ARV @12:33:19.73 delay 2 seconds @12:33:21.74 ARV.dial :air1 @12:33:22.06 delay 2 seconds @12:33:24.12 AS.reconfigure ASslow2 @12:33:24.15 AS.seek 0.506ml @12:33:30.62 delay 2 seconds @12:33:32.63 ARV.dial :MM1 @12:33:33.70 delay 2 seconds @12:33:35.71 Adding MM1 @12:33:35.77 AS.seek 0.524ml @12:33:54.42 delay 25 seconds @12:34:19.43 ARV.dial :PP1 @12:34:19.77 Adding PP1 @12:34:19.78 delay 2 seconds @12:34:21.83 AS.seek 0.530ml @12:34:28.08 delay 10 seconds @12:34:38.09 ARV.dial :air1 @12:34:38.41 adding ARV air @12:34:38.42 delay 2 seconds @12:34:40.47 AS.seek 0.536ml @12:34:46.79 delay 5 seconds @12:34:51.80 ARV.dial :carrier @12:34:52.11 delay 7 seconds @12:34:59.17 AS.seek 0.594ml @12:35:58.70 delay 5 seconds @12:36:03.71 ATV.dial :ASV @12:36:04.04 delay 2 seconds @12:36:06.10 AS.seek 0.600ml @12:36:12.40 delay 4 seconds @12:36:16.41 ATV.dial ARV @12:36:16.74 delay 2 seconds @12:36:18.75 ARV.dial :carrier @12:36:18.76 delay 2 seconds @12:36:20.81 AS.seek 0.650ml @12:37:12.02 delay 4 seconds @12:37:16.03 ATV.dial PCR @12:37:16.52 ASV.dial :waste1 @12:37:16.53 ARV.dial :air1 @12:37:16.88 Positioning Slug by 0.324ml @12:37:16.93 AS.seek 0.326ml @12:42:47.62 delay 5 seconds @12:42:52.63 ATV.dial ARV @12:42:53.23 ASV.dial :PCRlock @12:42:53.73 delay 5 seconds @12:42:58.74 Running ENTap Profile60C thermocycling @12:42:58.75 PCR.program Profile60C @12:42:58.85 Profile60C should complete in < 1:39:08 @12:42:58.86 PCR.run 2:08:55 @14:05:56.81 PCR.off @14:05:57.33 PCR for ENTap completed -- closed /var/log/esp/ENTap14mar1112h222ml.pcr @14:05:57.37 Cleaning PCR @14:05:57.38 AFV.select :carrierSolution @14:05:57.47 delay 2 seconds @14:05:59.48 AS.reconfigure ASconfig @14:05:59.51 AS.seek 0.500ml @14:06:09.91 delay 1 second @14:06:10.92 AFV.select :holdingCoil @14:06:11.01 ASV.dial :waste1 @14:06:11.49 ATV.dial :air3 @14:06:13.03 AS.reconfigure ASslow1 @14:06:13.07 AS.seek 0.510ml @14:06:15.37 delay 2 seconds @14:06:17.38 ATV.dial :bleach @14:06:17.88 AS.seek 0.810ml @14:07:19.28 delay 3 seconds @14:07:22.29 ATV.dial :air3 @14:07:22.61 delay 3 seconds @14:07:25.67 AS.seek 0.835ml @14:07:31.01 delay 3 seconds @14:07:34.02 ATV.dial :bleach @14:07:34.41 AS.seek 0.820ml @14:07:37.72 delay 3 seconds @14:07:40.73 ARV.dial :waste @14:07:41.33 delay 3 seconds @14:07:44.34 ATV.dial :ARV @14:07:45.07 AS.reconfigure ASconfig @14:07:45.11 AS.seek 0.470ml @14:08:05.78 AFV.select :carrierSolution @14:08:05.87 delay 2 seconds @14:08:07.88 AS.seek 1.000ml @14:08:39.09 delay 1 second @14:08:40.10 AFV.select :holdingCoil @14:08:40.19 delay 1 second @14:08:41.20 AS.reconfigure ASfast @14:08:41.23 AS.seek 0.000ml @14:09:08.96 AFV.select :carrierSolution @14:09:09.06 delay 2 seconds @14:09:11.07 AS.reconfigure ASconfig @14:09:11.10 AS.seek 1.000ml @14:10:09.71 delay 1 second @14:10:10.72 AFV.select :holdingCoil @14:10:10.81 delay 1 second @14:10:11.82 AS.reconfigure ASfast @14:10:11.85 AS.seek 0.000ml @14:10:39.56 AFV.select :carrierSolution @14:10:39.65 delay 2 seconds @14:10:41.66 AS.reconfigure ASconfig @14:10:41.69 AS.seek 1.000ml @14:11:40.29 delay 1 second @14:11:41.30 AFV.select :holdingCoil @14:11:41.39 delay 1 second @14:11:42.40 AS.reconfigure ASfast @14:11:42.43 AS.seek 0.000ml @14:12:10.16 ARV.dial between carrier and air1 @14:12:10.57 ATV.dial :air3 @14:12:11.58 AS.reconfigure ASslow1 @14:12:11.62 AS.seek 0.010ml @14:12:13.97 delay 2 seconds @14:12:15.98 ATV.dial :bleach @14:12:16.46 AS.seek 0.810ml @14:14:59.86 delay 3 seconds @14:15:02.87 ATV.dial :air3 @14:15:03.20 delay 3 seconds @14:15:06.26 AS.seek 0.835ml @14:15:11.59 delay 3 seconds @14:15:14.60 ATV.dial :bleach @14:15:14.99 AS.seek 0.820ml @14:15:18.31 delay 3 seconds @14:15:21.32 ATV.dial PCR @14:15:21.62 AS.reconfigure ASconfig @14:15:21.66 AS.seek empty @14:16:09.73 PCR.program :bleachProfile @14:16:09.79 PCR program should complete in < 2:45 @14:16:09.80 PCR.run 10:00 @14:19:08.00 PCR.off @14:19:08.03 AFV.select :carrierSolution @14:19:08.12 delay 2 seconds @14:19:10.13 AS.seek 1.000ml @14:20:08.76 delay 1 second @14:20:09.77 AFV.select :holdingCoil @14:20:09.86 delay 1 second @14:20:10.87 AS.reconfigure ASfast @14:20:10.90 AS.seek 0.000ml @14:20:38.63 delay 3 seconds @14:20:41.64 AFV.select :carrierSolution @14:20:41.73 delay 2 seconds @14:20:43.74 AS.reconfigure ASconfig @14:20:43.77 AS.seek 1.000ml @14:21:42.38 delay 1 second @14:21:43.39 AFV.select :holdingCoil @14:21:43.48 delay 1 second @14:21:44.49 AS.reconfigure ASfast @14:21:44.52 AS.seek 0.000ml @14:22:12.27 delay 3 seconds @14:22:15.28 AFV.select :carrierSolution @14:22:15.37 delay 2 seconds @14:22:17.38 AS.reconfigure ASconfig @14:22:17.41 AS.seek 1.000ml @14:23:16.00 delay 1 second @14:23:17.01 AFV.select :holdingCoil @14:23:17.10 delay 1 second @14:23:18.11 AS.reconfigure ASfast @14:23:18.14 AS.seek 0.000ml @14:23:45.88 delay 3 seconds @14:23:48.89 AFV.select :carrierSolution @14:23:48.98 delay 2 seconds @14:23:50.99 AS.reconfigure ASconfig @14:23:51.02 AS.seek 1.000ml @14:24:49.63 delay 1 second @14:24:50.64 AFV.select :holdingCoil @14:24:50.73 delay 1 second @14:24:51.74 AS.reconfigure ASfast @14:24:51.77 AS.seek 0.000ml @14:25:19.52 delay 3 seconds @14:25:22.53 AFV.select :carrierSolution @14:25:22.62 delay 2 seconds @14:25:24.63 AS.reconfigure ASconfig @14:25:24.66 AS.seek 1.000ml @14:26:23.24 delay 1 second @14:26:24.25 AFV.select :holdingCoil @14:26:24.34 delay 1 second @14:26:25.35 AS.reconfigure ASnorm @14:26:25.38 AS.seek 0.000ml @14:27:16.83 Setting up PCR for ENTem -- writing /var/log/esp/ENTem14mar1114h222ml.pcr @14:27:16.84 Building PCR Slug from ASV with MM2 mix, PP3 primer @14:27:16.85 Priming with carrier @14:27:16.86 ATV.dial ARV @14:27:17.38 ARV.dial :carrier @14:27:17.66 AS.seek 0.500ml @14:27:43.58 AFV.select :carrierSolution @14:27:43.67 delay 2 seconds @14:27:45.68 AS.reconfigure ASconfig @14:27:45.71 AS.seek 1.000ml @14:28:15.12 delay 1 second @14:28:16.13 AFV.select :holdingCoil @14:28:16.22 delay 1 second @14:28:17.23 ATV.dial :waste @14:28:19.23 AS.reconfigure ASnorm @14:28:19.27 AS.seek empty @14:29:10.78 AFV.select :carrierSolution @14:29:10.88 delay 2 seconds @14:29:12.89 AS.reconfigure ASconfig @14:29:12.92 AS.seek 0.500ml @14:29:42.40 delay 1 second @14:29:43.41 AFV.select :holdingCoil @14:29:43.50 Building Slug @14:29:43.51 ATV.dial :ARV @14:29:44.75 delay 2 seconds @14:29:46.76 ARV.dial :air1 @14:29:47.09 delay 2 seconds @14:29:49.15 AS.reconfigure ASslow2 @14:29:49.18 AS.seek 0.506ml @14:29:55.51 delay 2 seconds @14:29:57.52 ARV.dial :MM2 @14:29:58.73 delay 2 seconds @14:30:00.74 Adding MM2 @14:30:00.79 AS.seek 0.524ml @14:30:19.37 delay 25 seconds @14:30:44.38 ARV.dial :PP3 @14:30:44.71 Adding PP3 @14:30:44.72 delay 2 seconds @14:30:46.78 AS.seek 0.530ml @14:30:53.19 delay 10 seconds @14:31:03.20 ARV.dial :air2 @14:31:03.62 adding ARV air @14:31:03.63 delay 2 seconds @14:31:05.69 AS.seek 0.536ml @14:31:12.01 delay 5 seconds @14:31:17.02 ARV.dial :carrier @14:31:18.06 delay 7 seconds @14:31:25.12 AS.seek 0.594ml @14:32:24.41 delay 5 seconds @14:32:29.42 ATV.dial :ASV @14:32:29.74 delay 2 seconds @14:32:31.80 AS.seek 0.600ml @14:32:38.10 delay 4 seconds @14:32:42.11 ATV.dial ARV @14:32:42.44 delay 2 seconds @14:32:44.45 ARV.dial :carrier @14:32:44.46 delay 2 seconds @14:32:46.51 AS.seek 0.650ml @14:33:37.66 delay 4 seconds @14:33:41.67 ATV.dial PCR @14:33:42.18 ASV.dial :waste1 @14:33:42.19 ARV.dial :air1 @14:33:42.53 Positioning Slug by 0.324ml @14:33:42.58 AS.seek 0.326ml @14:39:13.21 delay 5 seconds @14:39:18.22 ATV.dial ARV @14:39:18.73 ASV.dial :PCRlock @14:39:19.21 delay 5 seconds @14:39:24.22 Running ENTem ProfileEM60C thermocycling @14:39:24.23 PCR.program ProfileEM60C @14:39:24.34 ProfileEM60C should complete in < 2:11:38 @14:39:24.35 PCR.run 2:49:32.5 @16:35:27.09 PCR.off @16:35:27.63 PCR for ENTem completed -- closed /var/log/esp/ENTem14mar1114h222ml.pcr @16:35:27.68 Cleaning PCR @16:35:27.69 AFV.select :carrierSolution @16:35:27.78 delay 2 seconds @16:35:29.79 AS.reconfigure ASconfig @16:35:29.82 AS.seek 0.500ml @16:35:40.22 delay 1 second @16:35:41.23 AFV.select :holdingCoil @16:35:41.32 ASV.dial :waste1 @16:35:41.80 ATV.dial :air3 @16:35:42.73 AS.reconfigure ASslow1 @16:35:42.77 AS.seek 0.510ml @16:35:45.05 delay 2 seconds @16:35:47.06 ATV.dial :bleach @16:35:47.54 AS.seek 0.810ml @16:36:48.99 delay 3 seconds @16:36:52.00 ATV.dial :air3 @16:36:52.33 delay 3 seconds @16:36:55.39 AS.seek 0.835ml @16:37:00.77 delay 3 seconds @16:37:03.78 ATV.dial :bleach @16:37:04.18 AS.seek 0.820ml @16:37:07.47 delay 3 seconds @16:37:10.48 ARV.dial :waste @16:37:10.99 delay 3 seconds @16:37:14.00 ATV.dial :ARV @16:37:14.73 AS.reconfigure ASconfig @16:37:14.77 AS.seek 0.470ml @16:37:35.45 AFV.select :carrierSolution @16:37:35.54 delay 2 seconds @16:37:37.55 AS.seek 1.000ml @16:38:08.80 delay 1 second @16:38:09.81 AFV.select :holdingCoil @16:38:09.90 delay 1 second @16:38:10.91 AS.reconfigure ASfast @16:38:10.94 AS.seek 0.000ml @16:38:38.69 AFV.select :carrierSolution @16:38:38.78 delay 2 seconds @16:38:40.79 AS.reconfigure ASconfig @16:38:40.82 AS.seek 1.000ml @16:39:39.45 delay 1 second @16:39:40.46 AFV.select :holdingCoil @16:39:40.55 delay 1 second @16:39:41.56 AS.reconfigure ASfast @16:39:41.59 AS.seek 0.000ml @16:40:09.30 AFV.select :carrierSolution @16:40:09.40 delay 2 seconds @16:40:11.41 AS.reconfigure ASconfig @16:40:11.44 AS.seek 1.000ml @16:41:10.02 delay 1 second @16:41:11.03 AFV.select :holdingCoil @16:41:11.12 delay 1 second @16:41:12.13 AS.reconfigure ASfast @16:41:12.16 AS.seek 0.000ml @16:41:39.91 ARV.dial between carrier and air1 @16:41:40.32 ATV.dial :air3 @16:41:41.35 AS.reconfigure ASslow1 @16:41:41.38 AS.seek 0.010ml @16:41:43.72 delay 2 seconds @16:41:45.73 ATV.dial :bleach @16:41:46.21 AS.seek 0.810ml @16:44:29.61 delay 3 seconds @16:44:32.62 ATV.dial :air3 @16:44:32.95 delay 3 seconds @16:44:36.01 AS.seek 0.835ml @16:44:41.36 delay 3 seconds @16:44:44.37 ATV.dial :bleach @16:44:44.77 AS.seek 0.820ml @16:44:48.12 delay 3 seconds @16:44:51.13 ATV.dial PCR @16:44:51.43 AS.reconfigure ASconfig @16:44:51.47 AS.seek empty @16:45:39.57 PCR.program :bleachProfile @16:45:39.62 PCR program should complete in < 2:45 @16:45:39.63 PCR.run 10:00 @16:48:37.82 PCR.off @16:48:37.85 AFV.select :carrierSolution @16:48:37.94 delay 2 seconds @16:48:39.95 AS.seek 1.000ml @16:49:38.61 delay 1 second @16:49:39.62 AFV.select :holdingCoil @16:49:39.71 delay 1 second @16:49:40.72 AS.reconfigure ASfast @16:49:40.75 AS.seek 0.000ml @16:50:08.50 delay 3 seconds @16:50:11.51 AFV.select :carrierSolution @16:50:11.60 delay 2 seconds @16:50:13.61 AS.reconfigure ASconfig @16:50:13.64 AS.seek 1.000ml @16:51:12.20 delay 1 second @16:51:13.21 AFV.select :holdingCoil @16:51:13.30 delay 1 second @16:51:14.31 AS.reconfigure ASfast @16:51:14.34 AS.seek 0.000ml @16:51:42.06 delay 3 seconds @16:51:45.07 AFV.select :carrierSolution @16:51:45.16 delay 2 seconds @16:51:47.17 AS.reconfigure ASconfig @16:51:47.20 AS.seek 1.000ml @16:52:45.82 delay 1 second @16:52:46.83 AFV.select :holdingCoil @16:52:46.92 delay 1 second @16:52:47.93 AS.reconfigure ASfast @16:52:47.96 AS.seek 0.000ml @16:53:15.70 delay 3 seconds @16:53:18.71 AFV.select :carrierSolution @16:53:18.80 delay 2 seconds @16:53:20.81 AS.reconfigure ASconfig @16:53:20.84 AS.seek 1.000ml @16:54:19.46 delay 1 second @16:54:20.47 AFV.select :holdingCoil @16:54:20.56 delay 1 second @16:54:21.57 AS.reconfigure ASfast @16:54:21.60 AS.seek 0.000ml @16:54:49.35 delay 3 seconds @16:54:52.36 AFV.select :carrierSolution @16:54:52.45 delay 2 seconds @16:54:54.46 AS.reconfigure ASconfig @16:54:54.49 AS.seek 1.000ml @16:55:53.65 delay 1 second @16:55:54.66 AFV.select :holdingCoil @16:55:54.75 delay 1 second @16:55:55.76 AS.reconfigure ASnorm @16:55:55.79 AS.seek 0.000ml @16:56:47.24 Setting up PCR for IPCap -- writing /var/log/esp/IPCap14mar1116h222ml.pcr @16:56:47.25 Building PCR Slug from ASV with MM1 mix, PP2 primer @16:56:47.26 Priming with carrier @16:56:47.27 ATV.dial ARV @16:56:47.87 ARV.dial :carrier @16:56:48.16 AS.seek 0.500ml @16:57:14.06 AFV.select :carrierSolution @16:57:14.16 delay 2 seconds @16:57:16.17 AS.reconfigure ASconfig @16:57:16.20 AS.seek 1.000ml @16:57:45.64 delay 1 second @16:57:46.65 AFV.select :holdingCoil @16:57:46.74 delay 1 second @16:57:47.75 ATV.dial :waste @16:57:49.01 AS.reconfigure ASnorm @16:57:49.04 AS.seek empty @16:58:40.53 AFV.select :carrierSolution @16:58:40.63 delay 2 seconds @16:58:42.64 AS.reconfigure ASconfig @16:58:42.67 AS.seek 0.500ml @16:59:12.15 delay 1 second @16:59:13.16 AFV.select :holdingCoil @16:59:13.25 Building Slug @16:59:13.26 ATV.dial :ARV @16:59:14.51 delay 2 seconds @16:59:16.52 ARV.dial :air1 @16:59:16.85 delay 2 seconds @16:59:18.91 AS.reconfigure ASslow2 @16:59:18.94 AS.seek 0.506ml @16:59:25.57 delay 2 seconds @16:59:27.58 ARV.dial :MM1 @16:59:28.17 delay 2 seconds @16:59:30.18 Adding MM1 @16:59:30.23 AS.seek 0.524ml @16:59:49.01 delay 25 seconds @17:00:14.02 ARV.dial :PP2 @17:00:14.45 Adding PP2 @17:00:14.46 delay 2 seconds @17:00:16.52 AS.seek 0.530ml @17:00:22.77 delay 10 seconds @17:00:32.78 ARV.dial :air2 @17:00:33.19 adding ARV air @17:00:33.20 delay 2 seconds @17:00:35.26 AS.seek 0.536ml @17:00:41.51 delay 5 seconds @17:00:46.52 ARV.dial :carrier @17:00:47.54 delay 7 seconds @17:00:54.60 AS.seek 0.594ml @17:01:53.91 delay 5 seconds @17:01:58.92 ATV.dial :ASV @17:01:59.33 delay 2 seconds @17:02:01.39 AS.seek 0.600ml @17:02:07.68 delay 4 seconds @17:02:11.69 ATV.dial ARV @17:02:12.02 delay 2 seconds @17:02:14.03 ARV.dial :carrier @17:02:14.04 delay 2 seconds @17:02:16.09 AS.seek 0.650ml @17:03:07.42 delay 4 seconds @17:03:11.43 ATV.dial PCR @17:03:11.92 ASV.dial :waste1 @17:03:11.93 ARV.dial :air1 @17:03:12.28 Positioning Slug by 0.324ml @17:03:12.33 AS.seek 0.326ml @17:08:42.90 delay 5 seconds @17:08:47.91 ATV.dial ARV @17:08:48.44 ASV.dial :PCRlock @17:08:48.93 delay 5 seconds @17:08:53.94 Running IPCap Profile59C thermocycling @17:08:53.95 PCR.program Profile59C @17:08:54.05 Profile59C should complete in < 1:39:08 @17:08:54.06 PCR.run 2:08:55 @18:32:40.47 PCR.off @18:32:41.01 PCR for IPCap completed -- closed /var/log/esp/IPCap14mar1116h222ml.pcr @18:32:41.06 Cleaning PCR @18:32:41.07 AFV.select :carrierSolution @18:32:41.17 delay 2 seconds @18:32:43.18 AS.reconfigure ASconfig @18:32:43.21 AS.seek 0.500ml @18:32:53.64 delay 1 second @18:32:54.65 AFV.select :holdingCoil @18:32:54.74 ASV.dial :waste1 @18:32:55.49 ATV.dial :air3 @18:32:56.43 AS.reconfigure ASslow1 @18:32:56.47 AS.seek 0.510ml @18:32:58.78 delay 2 seconds @18:33:00.79 ATV.dial :bleach @18:33:01.16 AS.seek 0.810ml @18:34:02.58 delay 3 seconds @18:34:05.59 ATV.dial :air3 @18:34:06.01 delay 3 seconds @18:34:09.07 AS.seek 0.835ml @18:34:14.42 delay 3 seconds @18:34:17.43 ATV.dial :bleach @18:34:17.92 AS.seek 0.820ml @18:34:21.23 delay 3 seconds @18:34:24.24 ARV.dial :waste @18:34:24.83 delay 3 seconds @18:34:27.84 ATV.dial :ARV @18:34:28.58 AS.reconfigure ASconfig @18:34:28.62 AS.seek 0.470ml @18:34:49.29 AFV.select :carrierSolution @18:34:49.38 delay 2 seconds @18:34:51.39 AS.seek 1.000ml @18:35:22.56 delay 1 second @18:35:23.57 AFV.select :holdingCoil @18:35:23.66 delay 1 second @18:35:24.67 AS.reconfigure ASfast @18:35:24.70 AS.seek 0.000ml @18:35:52.43 AFV.select :carrierSolution @18:35:52.52 delay 2 seconds @18:35:54.53 AS.reconfigure ASconfig @18:35:54.56 AS.seek 1.000ml @18:36:53.16 delay 1 second @18:36:54.17 AFV.select :holdingCoil @18:36:54.26 delay 1 second @18:36:55.27 AS.reconfigure ASfast @18:36:55.30 AS.seek 0.000ml @18:37:23.05 AFV.select :carrierSolution @18:37:23.14 delay 2 seconds @18:37:25.15 AS.reconfigure ASconfig @18:37:25.18 AS.seek 1.000ml @18:38:23.79 delay 1 second @18:38:24.80 AFV.select :holdingCoil @18:38:24.89 delay 1 second @18:38:25.90 AS.reconfigure ASfast @18:38:25.93 AS.seek 0.000ml @18:38:53.68 ARV.dial between carrier and air1 @18:38:54.10 ATV.dial :air3 @18:38:55.01 AS.reconfigure ASslow1 @18:38:55.05 AS.seek 0.010ml @18:38:57.41 delay 2 seconds @18:38:59.42 ATV.dial :bleach @18:38:59.79 AS.seek 0.810ml @18:41:43.20 delay 3 seconds @18:41:46.21 ATV.dial :air3 @18:41:46.56 delay 3 seconds @18:41:49.62 AS.seek 0.835ml @18:41:54.96 delay 3 seconds @18:41:57.97 ATV.dial :bleach @18:41:58.36 AS.seek 0.820ml @18:42:01.66 delay 3 seconds @18:42:04.67 ATV.dial PCR @18:42:04.98 AS.reconfigure ASconfig @18:42:05.02 AS.seek empty @18:42:53.15 PCR.program :bleachProfile @18:42:53.20 PCR program should complete in < 2:45 @18:42:53.21 PCR.run 10:00 @18:45:54.42 PCR.off @18:45:54.45 AFV.select :carrierSolution @18:45:54.54 delay 2 seconds @18:45:56.55 AS.seek 1.000ml @18:46:55.17 delay 1 second @18:46:56.18 AFV.select :holdingCoil @18:46:56.27 delay 1 second @18:46:57.28 AS.reconfigure ASfast @18:46:57.31 AS.seek 0.000ml @18:47:25.05 delay 3 seconds @18:47:28.06 AFV.select :carrierSolution @18:47:28.15 delay 2 seconds @18:47:30.16 AS.reconfigure ASconfig @18:47:30.19 AS.seek 1.000ml @18:48:28.76 delay 1 second @18:48:29.77 AFV.select :holdingCoil @18:48:29.86 delay 1 second @18:48:31.49 AS.reconfigure ASfast @18:48:31.52 AS.seek 0.000ml @18:48:59.26 delay 3 seconds @18:49:02.27 AFV.select :carrierSolution @18:49:02.36 delay 2 seconds @18:49:04.37 AS.reconfigure ASconfig @18:49:04.40 AS.seek 1.000ml @18:50:03.01 delay 1 second @18:50:04.02 AFV.select :holdingCoil @18:50:04.11 delay 1 second @18:50:05.12 AS.reconfigure ASfast @18:50:05.15 AS.seek 0.000ml @18:50:32.85 delay 3 seconds @18:50:35.86 AFV.select :carrierSolution @18:50:35.95 delay 2 seconds @18:50:37.96 AS.reconfigure ASconfig @18:50:37.99 AS.seek 1.000ml @18:51:36.60 delay 1 second @18:51:37.61 AFV.select :holdingCoil @18:51:37.70 delay 1 second @18:51:38.71 AS.reconfigure ASfast @18:51:38.82 AS.seek 0.000ml @18:52:06.52 delay 3 seconds @18:52:09.53 AFV.select :carrierSolution @18:52:09.62 delay 2 seconds @18:52:11.63 AS.reconfigure ASconfig @18:52:11.66 AS.seek 1.000ml @18:53:10.28 delay 1 second @18:53:11.29 AFV.select :holdingCoil @18:53:11.38 delay 1 second @18:53:12.39 AS.reconfigure ASnorm @18:53:12.42 AS.seek 0.000ml @18:54:03.89 Setting up PCR for IPCem -- writing /var/log/esp/IPCem14mar1118h222ml.pcr @18:54:03.90 Building PCR Slug from ASV with MM3 mix, PP5 primer @18:54:03.91 Priming with carrier @18:54:03.92 ATV.dial ARV @18:54:04.44 ARV.dial :carrier @18:54:04.72 AS.seek 0.500ml @18:54:30.59 AFV.select :carrierSolution @18:54:30.68 delay 2 seconds @18:54:32.69 AS.reconfigure ASconfig @18:54:32.72 AS.seek 1.000ml @18:55:02.19 delay 1 second @18:55:03.20 AFV.select :holdingCoil @18:55:03.29 delay 1 second @18:55:04.30 ATV.dial :waste @18:55:05.56 AS.reconfigure ASnorm @18:55:05.59 AS.seek empty @18:55:57.06 AFV.select :carrierSolution @18:55:57.17 delay 2 seconds @18:55:59.18 AS.reconfigure ASconfig @18:55:59.21 AS.seek 0.500ml @18:56:28.71 delay 1 second @18:56:29.72 AFV.select :holdingCoil @18:56:29.81 Building Slug @18:56:29.82 ATV.dial :ARV @18:56:31.05 delay 2 seconds @18:56:33.06 ARV.dial :air1 @18:56:33.38 delay 2 seconds @18:56:35.44 AS.reconfigure ASslow2 @18:56:35.47 AS.seek 0.506ml @18:56:41.87 delay 2 seconds @18:56:43.88 ARV.dial :MM3 @18:56:44.74 delay 2 seconds @18:56:46.75 Adding MM3 @18:56:46.80 AS.seek 0.524ml @18:57:05.29 delay 25 seconds @18:57:30.30 ARV.dial :PP5 @18:57:30.72 Adding PP5 @18:57:30.73 delay 2 seconds @18:57:32.79 AS.seek 0.530ml @18:57:39.04 delay 10 seconds @18:57:49.05 ARV.dial :air3 @18:57:49.38 adding ARV air @18:57:49.39 delay 2 seconds @18:57:51.45 AS.seek 0.536ml @18:57:57.79 delay 5 seconds @18:58:02.80 ARV.dial :carrier @18:58:03.83 delay 7 seconds @18:58:10.89 AS.seek 0.594ml @18:59:10.39 delay 5 seconds @18:59:15.40 ATV.dial :ASV @18:59:15.72 delay 2 seconds @18:59:17.78 AS.seek 0.600ml @18:59:24.19 delay 4 seconds @18:59:28.20 ATV.dial ARV @18:59:28.53 delay 2 seconds @18:59:30.54 ARV.dial :carrier @18:59:30.55 delay 2 seconds @18:59:32.60 AS.seek 0.650ml @19:00:23.81 delay 4 seconds @19:00:27.82 ATV.dial PCR @19:00:28.31 ASV.dial :waste1 @19:00:28.32 ARV.dial :air1 @19:00:28.66 Positioning Slug by 0.324ml @19:00:28.72 AS.seek 0.326ml @19:05:59.43 delay 5 seconds @19:06:04.44 ATV.dial ARV @19:06:04.96 ASV.dial :PCRlock @19:06:05.38 delay 5 seconds @19:06:10.39 Running IPCem ProfileEM59C thermocycling @19:06:10.40 PCR.program ProfileEM59C @19:06:10.51 ProfileEM59C should complete in < 2:11:38 @19:06:10.52 PCR.run 2:49:32.5 @21:01:50.17 PCR.off @21:01:50.71 PCR for IPCem completed -- closed /var/log/esp/IPCem14mar1118h222ml.pcr @21:01:50.72 Full MFB Decontamination Decon extraction column and decon AIV @21:01:50.74 SPE.hold 2:46:40,55.0C @21:01:50.76 ASV.dial :SPEdelivery @21:01:51.43 delay 20 seconds @21:02:11.44 AFV.select :carrierSolution @21:02:11.53 delay 2 seconds @21:02:13.54 AS.reconfigure ASconfig @21:02:13.57 AS.seek 0.500ml @21:02:23.99 delay 1 second @21:02:25.00 AFV.select :holdingCoil @21:02:25.09 ATV.dial :air1 @21:02:26.59 AS.reconfigure ASslow1 @21:02:26.62 AS.seek 0.520ml @21:02:30.95 ATV.dial :ASV @21:02:32.21 ASV.dial :SPErecovery @21:02:32.76 AS.seek 0.620ml @21:02:53.40 ATV.dial :waste @21:02:54.88 AS.reconfigure ASnorm @21:02:54.91 AS.seek empty @21:03:26.95 AFV.select :holdingCoil @21:03:26.96 ATV.dial :waste @21:03:26.97 AS.reconfigure ASconfig @21:03:27.00 AS.seek 0.000ml @21:03:27.29 AFV.select :carrierSolution @21:03:27.38 delay 2 seconds @21:03:29.39 AS.seek 0.500ml @21:03:58.80 delay 1 second @21:03:59.81 AFV.select :holdingCoil @21:03:59.90 ATV.dial :air3 @21:04:00.45 AS.reconfigure ASslow1 @21:04:00.49 AS.seek 0.520ml @21:04:04.83 ATV.dial :air3 @21:04:04.89 AS.seek 0.530ml @21:04:07.15 delay 2 seconds @21:04:09.16 ATV.dial :bleach @21:04:09.54 AS.seek 0.710ml @21:04:46.47 delay 3 seconds @21:04:49.48 ATV.dial :air3 @21:04:49.90 delay 3 seconds @21:04:52.96 AS.seek 0.735ml @21:04:58.32 delay 3 seconds @21:05:01.33 ATV.dial :bleach @21:05:01.71 AS.seek 0.720ml @21:05:05.00 delay 3 seconds @21:05:08.01 ATV.dial ASV @21:05:08.88 ASV.dial :SPErecovery @21:05:08.94 AS.seek 0.365ml @21:06:21.56 ATV.dial :waste @21:06:23.03 AS.reconfigure ASnorm @21:06:23.06 AS.seek empty @21:06:42.10 ATV.dial :air3 @21:06:42.67 AS.reconfigure ASslow1 @21:06:42.71 AS.seek 0.009ml @21:06:45.05 delay 2 seconds @21:06:47.06 ATV.dial :bleach @21:06:47.44 AS.seek 0.579ml @21:08:43.95 delay 3 seconds @21:08:46.96 ATV.dial :air3 @21:08:47.30 delay 3 seconds @21:08:50.36 AS.seek 0.604ml @21:08:55.73 delay 3 seconds @21:08:58.74 ATV.dial :bleach @21:08:59.13 AS.seek 0.589ml @21:09:02.43 delay 3 seconds @21:09:05.44 ATV.dial :ASV @21:09:06.31 ASV.dial :wasteR @21:09:06.80 AS.seek 0.000ml @21:11:07.20 delay 1:00 @21:12:07.21 ATV.dial ASV @21:12:07.22 AFV.select :carrierSolution @21:12:07.31 delay 2 seconds @21:12:09.32 AS.reconfigure ASconfig @21:12:09.35 AS.seek 1.000ml @21:13:08.59 delay 1 second @21:13:09.60 AFV.select :holdingCoil @21:13:09.69 delay 1 second @21:13:10.70 ASV.dial :wasteR @21:13:10.71 AS.reconfigure ASslow1 @21:13:10.74 AS.seek 0.000ml @21:16:34.99 delay 10 seconds @21:16:45.00 ATV.dial :air3 @21:16:46.20 AS.seek 0.010ml @21:16:48.51 delay 2 seconds @21:16:50.52 ATV.dial :bleach @21:16:51.01 AS.seek 0.580ml @21:18:47.52 delay 3 seconds @21:18:50.53 ATV.dial :air3 @21:18:50.86 delay 3 seconds @21:18:53.92 AS.seek 0.605ml @21:18:59.27 delay 3 seconds @21:19:02.28 ATV.dial :bleach @21:19:02.68 AS.seek 0.590ml @21:19:05.99 delay 3 seconds @21:19:09.00 ATV.dial ASV @21:19:09.96 ASV.dial :SPEdelivery @21:19:10.28 AS.seek 0.000ml @21:21:10.84 delay 1:00 @21:22:10.85 AFV.select :carrierSolution @21:22:10.94 delay 2 seconds @21:22:12.95 AS.reconfigure ASconfig @21:22:12.98 AS.seek 1.000ml @21:23:11.56 delay 1 second @21:23:12.57 AFV.select :holdingCoil @21:23:12.66 delay 1 second @21:23:13.67 ATV.dial ASV @21:23:13.68 ASV.dial :SPEdelivery @21:23:13.69 AS.reconfigure ASslow1 @21:23:13.72 AS.seek 0.000ml @21:26:37.97 delay 10 seconds @21:26:47.98 ATV.dial :air2 @21:26:48.75 AS.reconfigure ASslow0 @21:26:48.79 AS.seek 0.800ml @21:28:10.69 delay 3 seconds @21:28:13.70 ATV.dial :ASV @21:28:14.42 ASV.dial :SPEdelivery @21:28:14.44 AS.reconfigure ASslow1 @21:28:14.47 AS.seek 0.030ml @21:30:51.79 delay 20 seconds @21:31:11.80 ATV.dial :waste,via => air3 @21:31:13.24 AS.reconfigure ASconfig @21:31:13.27 AS.seek empty @21:31:15.33 SPE.coast @21:31:15.41 Removing Bleach from Mixing Coil @21:31:15.42 AFV.select :carrierSolution @21:31:15.51 delay 2 seconds @21:31:17.52 AS.seek 0.500ml @21:31:47.01 delay 1 second @21:31:48.02 AFV.select :holdingCoil @21:31:48.11 ATV.dial :ASV @21:31:49.57 ASV.dial :SPErecovery @21:31:50.53 AS.reconfigure ASslow1 @21:31:50.57 AS.seek 0.730ml @21:32:37.77 ASV.dial :wasteD @21:32:38.44 AS.reconfigure ASnorm @21:32:38.47 AS.seek 0.000ml @21:33:16.07 AFV.select :carrierSolution @21:33:16.16 delay 2 seconds @21:33:18.17 AS.reconfigure ASconfig @21:33:18.20 AS.seek 0.500ml @21:33:47.64 delay 1 second @21:33:48.65 AFV.select :holdingCoil @21:33:48.74 ATV.dial ASV @21:33:48.75 ASV.dial :SPErecovery @21:33:49.46 AS.reconfigure ASslow0 @21:33:49.49 AS.seek 0.300ml @21:34:10.17 ATV.dial :air2 @21:34:10.93 AS.reconfigure ASslow1 @21:34:10.97 AS.seek 0.320ml @21:34:15.33 ATV.dial ASV @21:34:16.09 AS.reconfigure ASslow0 @21:34:16.13 AS.seek 0.570ml @21:34:41.94 ASV.dial :wasteD @21:34:42.60 AS.reconfigure ASnorm @21:34:42.63 AS.seek 0.000ml @21:35:12.11 AFV.select :carrierSolution @21:35:12.20 delay 2 seconds @21:35:14.21 AS.reconfigure ASconfig @21:35:14.24 AS.seek 0.500ml @21:35:43.67 delay 1 second @21:35:44.68 AFV.select :holdingCoil @21:35:44.77 ATV.dial ASV @21:35:44.78 ASV.dial :SPErecovery @21:35:45.48 AS.reconfigure ASslow0 @21:35:45.52 AS.seek 0.300ml @21:36:06.20 ATV.dial :air2 @21:36:06.96 AS.reconfigure ASslow1 @21:36:07.00 AS.seek 0.320ml @21:36:11.36 ATV.dial ASV @21:36:12.21 AS.reconfigure ASslow0 @21:36:12.25 AS.seek 0.570ml @21:36:38.06 ASV.dial :wasteD @21:36:38.99 AS.reconfigure ASnorm @21:36:39.02 AS.seek 0.000ml @21:37:08.50 ATV.dial :air2 @21:37:09.21 AS.reconfigure ASslow0 @21:37:09.24 AS.seek 0.300ml @21:37:40.12 ATV.dial :ASV @21:37:40.84 ASV.dial :wasteD @21:37:40.85 AS.reconfigure ASconfig @21:37:40.89 AS.seek 0.050ml @21:37:55.74 ATV.dial :waste @21:37:57.18 AS.seek 0.000ml @21:38:00.37 ASV.dial :SPEdelivery @21:38:01.21 deconLysateINT @21:38:01.22 ATV.dial :air3 @21:38:01.86 AS.reconfigure ASslow1 @21:38:01.90 AS.seek 0.010ml @21:38:04.24 delay 2 seconds @21:38:06.25 ATV.dial :bleach @21:38:06.62 AS.seek 0.960ml @21:41:20.61 delay 3 seconds @21:41:23.62 ATV.dial :air3 @21:41:24.03 delay 3 seconds @21:41:27.09 AS.seek 0.985ml @21:41:32.99 delay 3 seconds @21:41:36.00 ATV.dial :bleach @21:41:36.49 AS.seek 0.970ml @21:41:39.80 delay 3 seconds @21:41:42.81 ATV.dial :AIV @21:41:43.89 AIV.dial :coilToMFB @21:41:43.90 AWV.select :waste @21:41:43.91 AFV.select :carrierSolution @21:41:44.00 delay 2 seconds @21:41:46.01 AS.reconfigure ASconfig @21:41:46.04 AS.seek 1.000ml @21:41:48.14 delay 1 second @21:41:49.15 AFV.select :holdingCoil @21:41:49.24 delay 1 second @21:41:50.25 AS.reconfigure ASslow0 @21:41:50.28 AS.seek empty @21:43:32.70 AFV.select :carrierSolution @21:43:32.79 delay 2 seconds @21:43:34.80 AS.reconfigure ASconfig @21:43:34.83 AS.seek 1.000ml @21:44:33.50 delay 1 second @21:44:34.51 AFV.select :holdingCoil @21:44:34.60 delay 1 second @21:44:35.61 AS.reconfigure ASslow0 @21:44:35.64 AS.seek empty @21:46:18.04 AFV.select :carrierSolution @21:46:18.13 delay 2 seconds @21:46:20.14 AS.reconfigure ASconfig @21:46:20.17 AS.seek 1.000ml @21:47:18.86 delay 1 second @21:47:19.87 AFV.select :holdingCoil @21:47:19.96 delay 1 second @21:47:20.97 AS.reconfigure ASslow0 @21:47:21.00 AS.seek empty @21:49:03.40 ATV.dial :air2 @21:49:03.96 AS.reconfigure ASslow1 @21:49:04.00 AS.seek 0.009ml @21:49:06.36 AS.reconfigure ASslow0 @21:49:06.40 AS.seek 1.000ml @21:50:47.78 ATV.dial AIV @21:50:48.41 AS.seek 0.020ml @21:52:28.68 ATV.dial :waste @21:52:30.03 AS.seek 0.000ml @21:52:32.35 AWV.select :air @21:52:32.49 Cleaning PCR @21:52:32.50 AFV.select :carrierSolution @21:52:32.59 delay 2 seconds @21:52:34.60 AS.reconfigure ASconfig @21:52:34.63 AS.seek 0.500ml @21:53:04.05 delay 1 second @21:53:05.06 AFV.select :holdingCoil @21:53:05.15 ASV.dial :waste1 @21:53:05.48 ATV.dial :air3 @21:53:06.05 AS.reconfigure ASslow1 @21:53:06.09 AS.seek 0.510ml @21:53:08.40 delay 2 seconds @21:53:10.41 ATV.dial :bleach @21:53:10.79 AS.seek 0.810ml @21:54:12.19 delay 3 seconds @21:54:15.20 ATV.dial :air3 @21:54:15.54 delay 3 seconds @21:54:18.60 AS.seek 0.835ml @21:54:23.94 delay 3 seconds @21:54:26.95 ATV.dial :bleach @21:54:27.34 AS.seek 0.820ml @21:54:30.66 delay 3 seconds @21:54:33.67 ARV.dial :waste @21:54:34.29 delay 3 seconds @21:54:37.30 ATV.dial :ARV @21:54:38.12 AS.reconfigure ASconfig @21:54:38.16 AS.seek 0.470ml @21:54:58.85 AFV.select :carrierSolution @21:54:58.94 delay 2 seconds @21:55:00.95 AS.seek 1.000ml @21:55:32.17 delay 1 second @21:55:33.18 AFV.select :holdingCoil @21:55:33.27 delay 1 second @21:55:34.28 AS.reconfigure ASfast @21:55:34.31 AS.seek 0.000ml @21:56:02.05 AFV.select :carrierSolution @21:56:02.14 delay 2 seconds @21:56:04.15 AS.reconfigure ASconfig @21:56:04.18 AS.seek 1.000ml @21:57:02.75 delay 1 second @21:57:03.76 AFV.select :holdingCoil @21:57:03.99 delay 1 second @21:57:05.00 AS.reconfigure ASfast @21:57:05.03 AS.seek 0.000ml @21:57:32.76 AFV.select :carrierSolution @21:57:32.85 delay 2 seconds @21:57:34.86 AS.reconfigure ASconfig @21:57:34.89 AS.seek 1.000ml @21:58:33.46 delay 1 second @21:58:34.47 AFV.select :holdingCoil @21:58:34.56 delay 1 second @21:58:35.57 AS.reconfigure ASfast @21:58:35.60 AS.seek 0.000ml @21:59:03.34 ARV.dial between carrier and air1 @21:59:03.75 ATV.dial :air3 @21:59:04.77 AS.reconfigure ASslow1 @21:59:04.81 AS.seek 0.010ml @21:59:07.14 delay 2 seconds @21:59:09.15 ATV.dial :bleach @21:59:09.64 AS.seek 0.810ml @22:01:53.01 delay 3 seconds @22:01:56.02 ATV.dial :air3 @22:01:56.35 delay 3 seconds @22:01:59.41 AS.seek 0.835ml @22:02:04.76 delay 3 seconds @22:02:07.77 ATV.dial :bleach @22:02:08.16 AS.seek 0.820ml @22:02:11.47 delay 3 seconds @22:02:14.48 ATV.dial PCR @22:02:14.79 AS.reconfigure ASconfig @22:02:14.83 AS.seek empty @22:03:02.94 PCR.program :bleachProfile @22:03:02.99 PCR program should complete in < 2:45 @22:03:03.00 PCR.run 10:00 @22:06:07.80 PCR.off @22:06:07.83 AFV.select :carrierSolution @22:06:07.92 delay 2 seconds @22:06:09.93 AS.seek 1.000ml @22:07:08.55 delay 1 second @22:07:09.56 AFV.select :holdingCoil @22:07:09.65 delay 1 second @22:07:10.66 AS.reconfigure ASfast @22:07:10.69 AS.seek 0.000ml @22:07:38.43 delay 3 seconds @22:07:41.44 AFV.select :carrierSolution @22:07:41.53 delay 2 seconds @22:07:43.54 AS.reconfigure ASconfig @22:07:43.57 AS.seek 1.000ml @22:08:42.14 delay 1 second @22:08:43.15 AFV.select :holdingCoil @22:08:43.24 delay 1 second @22:08:44.25 AS.reconfigure ASfast @22:08:44.29 AS.seek 0.000ml @22:09:12.03 delay 3 seconds @22:09:15.04 AFV.select :carrierSolution @22:09:15.13 delay 2 seconds @22:09:17.14 AS.reconfigure ASconfig @22:09:17.17 AS.seek 1.000ml @22:10:15.77 delay 1 second @22:10:16.78 AFV.select :holdingCoil @22:10:16.87 delay 1 second @22:10:17.88 AS.reconfigure ASfast @22:10:17.91 AS.seek 0.000ml @22:10:45.64 delay 3 seconds @22:10:48.65 AFV.select :carrierSolution @22:10:48.74 delay 2 seconds @22:10:50.75 AS.reconfigure ASconfig @22:10:50.78 AS.seek 1.000ml @22:11:49.39 delay 1 second @22:11:50.40 AFV.select :holdingCoil @22:11:50.49 delay 1 second @22:11:51.50 AS.reconfigure ASfast @22:11:51.53 AS.seek 0.000ml @22:12:19.26 delay 3 seconds @22:12:22.27 AFV.select :carrierSolution @22:12:22.36 delay 2 seconds @22:12:24.37 AS.reconfigure ASconfig @22:12:24.40 AS.seek 1.000ml @22:13:22.98 delay 1 second @22:13:23.99 AFV.select :holdingCoil @22:13:24.08 delay 1 second @22:13:25.09 AS.reconfigure ASnorm @22:13:25.12 AS.seek 0.000ml @22:14:16.56 AFV.select :carrierSolution @22:14:16.65 delay 2 seconds @22:14:18.66 AS.reconfigure ASconfig @22:14:18.69 AS.seek 1.000ml @22:15:17.30 delay 1 second @22:15:18.31 AFV.select :holdingCoil @22:15:18.40 delay 1 second @22:15:19.41 ATV.dial :waste @22:15:20.31 AS.reconfigure ASnorm @22:15:20.34 AS.seek 0.500ml @22:15:46.27 ATV.dial :waste2 @22:15:47.36 AS.seek 0.000ml @22:16:13.26
Cleaning PCR @22:16:13.27 AFV.select :carrierSolution @22:16:13.36 delay 2 seconds @22:16:15.37 AS.reconfigure ASconfig @22:16:15.40 AS.seek 0.500ml @22:16:44.82 delay 1 second @22:16:45.83 AFV.select :holdingCoil @22:16:45.92 ASV.dial :waste1 @22:16:45.93 ATV.dial :air3 @22:16:47.39 AS.reconfigure ASslow1 @22:16:47.43 AS.seek 0.510ml @22:16:49.71 delay 2 seconds @22:16:51.72 ATV.dial :bleach @22:16:52.10 AS.seek 0.810ml @22:17:53.53 delay 3 seconds @22:17:56.54 ATV.dial :air3 @22:17:56.95 delay 3 seconds @22:18:00.01 AS.seek 0.835ml @22:18:05.34 delay 3 seconds @22:18:08.35 ATV.dial :bleach @22:18:08.83 AS.seek 0.820ml @22:18:12.14 delay 3 seconds @22:18:15.15 ARV.dial :waste @22:18:15.64 delay 3 seconds @22:18:18.65 ATV.dial :ARV @22:18:19.38 AS.reconfigure ASconfig @22:18:19.42 AS.seek 0.470ml @22:18:40.11 AFV.select :carrierSolution @22:18:40.20 delay 2 seconds @22:18:42.21 AS.seek 1.000ml @22:19:13.42 delay 1 second @22:19:14.43 AFV.select :holdingCoil @22:19:14.52 delay 1 second @22:19:15.53 AS.reconfigure ASfast @22:19:15.56 AS.seek 0.000ml @22:19:43.28 AFV.select :carrierSolution @22:19:43.37 delay 2 seconds @22:19:45.38 AS.reconfigure ASconfig @22:19:45.41 AS.seek 1.000ml @22:20:44.00 delay 1 second @22:20:45.01 AFV.select :holdingCoil @22:20:45.11 delay 1 second @22:20:46.12 AS.reconfigure ASfast @22:20:46.15 AS.seek 0.000ml @22:21:13.90 AFV.select :carrierSolution @22:21:13.99 delay 2 seconds @22:21:16.00 AS.reconfigure ASconfig @22:21:16.03 AS.seek 1.000ml @22:22:14.66 delay 1 second @22:22:15.67 AFV.select :holdingCoil @22:22:15.76 delay 1 second @22:22:16.77 AS.reconfigure ASfast @22:22:16.80 AS.seek 0.000ml @22:22:44.52 ARV.dial between carrier and air1 @22:22:44.92 ATV.dial :air3 @22:22:45.93 AS.reconfigure ASslow1 @22:22:45.97 AS.seek 0.010ml @22:22:48.33 delay 2 seconds @22:22:50.34 ATV.dial :bleach @22:22:50.72 AS.seek 0.810ml @22:25:34.09 delay 3 seconds @22:25:37.10 ATV.dial :air3 @22:25:37.51 delay 3 seconds @22:25:40.57 AS.seek 0.835ml @22:25:45.92 delay 3 seconds @22:25:48.93 ATV.dial :bleach @22:25:49.33 AS.seek 0.820ml @22:25:52.65 delay 3 seconds @22:25:55.66 ATV.dial PCR @22:25:55.97 AS.reconfigure ASconfig @22:25:56.00 AS.seek empty @22:26:44.07 PCR.program :bleachProfile @22:26:44.13 PCR program should complete in < 2:45 @22:26:44.14 PCR.run 10:00 @22:29:48.90 PCR.off @22:29:48.93 AFV.select :carrierSolution @22:29:49.02 delay 2 seconds @22:29:51.03 AS.seek 1.000ml @22:30:49.66 delay 1 second @22:30:50.67 AFV.select :holdingCoil @22:30:50.76 delay 1 second @22:30:51.77 AS.reconfigure ASfast @22:30:51.80 AS.seek 0.000ml @22:31:19.55 delay 3 seconds @22:31:22.56 AFV.select :carrierSolution @22:31:22.65 delay 2 seconds @22:31:24.66 AS.reconfigure ASconfig @22:31:24.69 AS.seek 1.000ml @22:32:23.30 delay 1 second @22:32:24.31 AFV.select :holdingCoil @22:32:24.40 delay 1 second @22:32:25.41 AS.reconfigure ASfast @22:32:25.44 AS.seek 0.000ml @22:32:53.19 delay 3 seconds @22:32:56.20 AFV.select :carrierSolution @22:32:56.29 delay 2 seconds @22:32:58.30 AS.reconfigure ASconfig @22:32:58.33 AS.seek 1.000ml @22:33:56.95 delay 1 second @22:33:57.96 AFV.select :holdingCoil @22:33:58.05 delay 1 second @22:33:59.06 AS.reconfigure ASfast @22:33:59.10 AS.seek 0.000ml @22:34:26.82 delay 3 seconds @22:34:29.83 AFV.select :carrierSolution @22:34:29.92 delay 2 seconds @22:34:31.93 AS.reconfigure ASconfig @22:34:31.96 AS.seek 1.000ml @22:35:30.57 delay 1 second @22:35:31.58 AFV.select :holdingCoil @22:35:31.67 delay 1 second @22:35:32.68 AS.reconfigure ASfast @22:35:32.71 AS.seek 0.000ml @22:36:00.45 delay 3 seconds @22:36:03.46 AFV.select :carrierSolution @22:36:03.55 delay 2 seconds @22:36:05.56 AS.reconfigure ASconfig @22:36:05.59 AS.seek 1.000ml @22:37:04.18 delay 1 second @22:37:05.19 AFV.select :holdingCoil @22:37:05.28 delay 1 second @22:37:06.29 AS.reconfigure ASnorm @22:37:06.32 AS.seek 0.000ml @22:37:57.77 Completed {wqPCR} begun 08:00:31 @22:37:57.80 Asleep til 08:00PDT12-Mar-14 @22:37:57.81 AS.stop @22:37:57.82
Sleeping until 08:00PDT12-Mar-14 @22:37:57.85 GoodNews.email "Then, in tube 3: wqPCR\n/home/esp/esp2local/mission/14mar05sccwrpDeploy.rb:64",Subject => "Asleep until 08:00PDT12-Mar-14" @22:37:58.81 CC.stop @22:37:58.95 CS.stop @22:37:58.97 PC.stop @22:37:59.00 PS.stop @22:37:59.03 Forearm.stop @22:37:59.05 Elbow.stop @22:37:59.08 SS.stop @22:37:59.15 Sleepy.power :raw,:OFF @22:37:59.16 Sleepy.power :dwarf,:OFF Shutdown @08:00:31.80PDT12-Mar-14
Powering up Dwarves @08:00:36.90 Sleepy.power :raw,:ON @08:00:37.36 SPE.configure SPEconfig @08:00:37.38 AS.configure ASconfig @08:00:37.42 CC.configure CCconfig @08:00:37.45 CS.configure CSconfig @08:00:37.48 PC.configure PCconfig @08:00:37.51 PS.configure PSconfig @08:00:37.54 Forearm.configure LooseForearm @08:00:37.57 Elbow.configure LooseElbow @08:00:37.60 SC.configure SCloose @08:00:37.63 SE.configure SEconfig @08:00:37.66 SS.configure SSconfig @08:00:37.69 CH.configure CHconfig @08:00:37.71 PH.configure PHconfig @08:00:37.74 Valve.reset @08:00:38.40 PCR.off @08:00:38.42 PCR.pidtemp= 50,0.1,0,300,25,25 @08:00:38.45 PCR.caltemp= 6.6661,0.0344,0.0 @08:00:38.56 Intake.select :close @08:00:38.57 Exhaust.select :close @08:00:38.58 CTV.dial :air @08:00:39.19 PSV.dial :air @08:00:39.22
PC.reconfigure PCconfig @08:00:39.28 PC.home.seek open @08:00:39.30 CSV.dial :waste1 @08:00:39.66 PS.reconfigure PSconfig @08:00:39.70 PS.home.seek 0.000ml @08:00:39.96 SSV.dial :spit,avoiding => exhaust @08:00:40.75 CS.reconfigure CSfast @08:00:40.78 SS.reconfigure SSconfig @08:00:40.87 SS.home.seek 0.00ml @08:00:40.89 CS.home.seek 0.000ml @08:00:40.97 CC.reconfigure CCconfig @08:00:41.01 CC.home.seek open @08:00:49.91 PS.coast @08:00:50.10
CC.seek open @08:00:50.12 PC.seek open @08:00:50.27
Forearm.reconfigure LooseForearm @08:00:50.30 Forearm.home.seek retract @08:00:51.03 Hand.grip @08:00:51.05
Elbow.jog -1500 @08:00:51.57 Elbow.reconfigure LooseElbow @08:00:51.61 Elbow.home.seek clear @08:00:56.34 SE.reconfigure SEconfig @08:00:56.37 SE.home.seek down @08:00:56.90 SC.reconfigure SCsloppy @08:00:56.94 SC.home.seek initial @08:01:00.71 Forearm.seek retract @08:01:00.76 Elbow.seek hammer @08:01:01.92 Forearm.reconfigure TightForearm @08:01:01.97 Forearm.seek 2 @08:01:02.97 Forearm.seek retract @08:01:04.09 Forearm.reconfigure LooseForearm @08:01:04.59PDT12-Mar-14 ===> STARTED wqPCR (phase #9) <=== @08:01:04.60 Starting initialPurge @08:01:04.61 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:01:04.66 Hand.release @08:01:05.51 Elbow.reconfigure TightElbow @08:01:05.54 Elbow.seek FlushPuck::Garage @08:01:07.32 Elbow.reconfigure LooseElbow @08:01:07.41 Forearm.seek garage @08:01:08.42 Hand.grip @08:01:09.26 Forearm.seek retract @08:01:10.28 Elbow.reconfigure TightElbow @08:01:10.32 Elbow.seek CC @08:01:13.12 Elbow.reconfigure LooseElbow @08:01:13.21 Forearm.reconfigure TightForearm @08:01:13.24 Forearm.seek CC @08:01:14.33 CC.seek closed @08:01:18.07 Collection Clamp onSpring/offSpring for 5/22 samples, on/off torque 1.92 @08:01:18.12 Hand.release @08:01:18.85 Forearm.reconfigure LooseForearm @08:01:18.88 Forearm.seek retract @08:01:19.85 Flushing the Collection Valve::Manifold @08:01:19.95 CS Pulling 5ml of flush CSV.dial CSR @08:01:21.21 CSR.select :flush @08:01:21.31 CS.reconfigure CSconfig @08:01:21.34 CS.seek 5.000ml @08:01:34.82 delay 5 seconds @08:01:39.83 CSR.select :air @08:01:39.97 CS.reconfigure CSfast @08:01:40.00 CS.seek 7.499ml @08:01:46.63 CSV.dial :waste @08:01:47.89 CS.reconfigure CSconfig @08:01:47.93 CS.seek 0.000ml @08:02:07.91 Purging external Intake @08:02:08.00 CSV.dial :flush @08:02:08.50 CS.reconfigure CSfast @08:02:08.54 CS.seek 10.000ml @08:02:32.32 delay 5 seconds @08:02:37.33 CSV.dial CBV @08:02:37.35
CBV.dial CSV @08:02:38.14 CTV.dial :intake @08:02:38.89 Intake.select :open @08:02:38.98 CS.seek 0.000ml @08:03:03.06 Intake.select :close @08:03:03.15 CTV.dial :air @08:03:03.89 Flushing upper manifold @08:03:03.99 CSV.dial :flush @08:03:04.78 CS.seek 5.000ml @08:03:17.03 delay 5 seconds @08:03:22.04 CSV.dial :air @08:03:22.72 CS.seek 7.001ml @08:03:28.25 CSV.dial CBV @08:03:28.27
CBV.dial CSV @08:03:29.27 CTV.dial :air @08:03:29.29 CS.seek 0.000ml @08:03:46.22 delay 7 seconds @08:03:53.23 CS.seek 3.000ml @08:04:00.97 CSV.dial :waste @08:04:01.48 CS.seek 0.000ml @08:04:09.30 CSV.dial :flush @08:04:09.74 CS.seek 3.000ml @08:04:17.44 delay 5 seconds @08:04:22.45 CSV.dial :air @08:04:22.92 CS.seek 4.999ml @08:04:28.37 CSV.dial :air @08:04:28.38 CS.seek 0.000ml @08:04:40.75 delay 7 seconds @08:04:47.76 CS.seek 3.000ml @08:04:55.47 CSV.dial :waste @08:04:56.26 CS.seek 0.000ml @08:05:03.93 Garaging FlushPuck in Collection Clamp @08:05:03.99 Elbow.reconfigure TightElbow @08:05:04.02 Elbow.seek CC @08:05:04.23 Elbow.reconfigure LooseElbow @08:05:04.32 Forearm.seek CC @08:05:05.31 CC.seek sealed @08:05:06.25 Hand.grip @08:05:06.92 CC.seek open @08:05:10.21 Forearm.seek retract @08:05:11.15 Elbow.reconfigure TightElbow @08:05:11.19 Elbow.seek garage @08:05:14.53 Elbow.reconfigure LooseElbow @08:05:14.62 Forearm.reconfigure TightForearm @08:05:14.65 Forearm.seek garage @08:05:15.82 Hand.release @08:05:16.59 Forearm.reconfigure LooseForearm @08:05:16.62 Forearm.seek retract @08:05:17.74 Starting mfb @08:05:17.75 pcrBAC sampling at most 1000ml, wcr at most 1000ml @08:05:17.76 BACfans.email "Can@08:01:04, 14.9C, 68% humidity, 14.2psia, 13.714V, 0.652A, 0.652A avg, 8.95W\nCTD data unavailable\nISUS data unavailable",Subject => "pcrBAC sampling at most 1000ml, wcr at most 1000ml" @08:05:17.79
Loading sh1 puck from tube 3 into Collection Clamp @08:05:19.77 Readying the MFB @08:05:19.78 Homing MFB Syringe @08:05:19.79 AFV.select :holdingCoil @08:05:19.98
SC.reconfigure SCtight @08:05:20.04 SC.seek 3 @08:05:20.06 ATV.dial :air2 @08:05:21.46 AS.reconfigure ASconfig @08:05:21.49 AS.home.seek home @08:05:22.22
SC.reconfigure SCloose @08:05:22.40 Elbow.reconfigure TightElbow @08:05:22.43 Elbow.seek 3 @08:05:23.18 AFV.select :carrierSolution @08:05:23.28 delay 2 seconds @08:05:24.29
Forearm.reconfigure TightForearm @08:05:24.32 Forearm.seek 3 + 800 counts @08:05:25.29 AS.seek 1.000ml @08:05:25.39
SE.reconfigure SEpick @08:05:25.44 SE.seek beamBreak @08:05:26.47 SE.configure SEconfig @08:05:26.60 Elbow.seek 3 @08:05:26.75 Forearm.seek 3 + 300 counts @08:05:26.98 Forearm.reconfigure LooseForearm @08:05:27.02 Hand.grip @08:05:27.82 SE.seek down @08:05:27.94
Forearm.seek retract @08:05:28.75 SE.coast @08:05:28.89
Elbow.seek CC @08:05:30.37 Elbow.reconfigure LooseElbow @08:05:30.45 Forearm.reconfigure TightForearm @08:05:30.48 Forearm.seek CC @08:05:31.55 CC.seek closed @08:05:35.33 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.98 @08:05:35.34 Hand.release @08:05:36.09 Forearm.reconfigure LooseForearm @08:05:36.12 Forearm.seek retract @08:05:37.10 SC.coast @08:05:37.13 collectSample 1000ml w/bubble point of 55psi Priming 7ml @08:05:37.14 Sleepy.power :sampler,:ON @08:05:37.15 Intake.select :close Exhaust.select :close @08:05:37.16 SS.hold @08:05:37.32 Calibrating pressure and temperature sensors @08:05:37.33 CTV.dial :air @08:05:37.34 SSV.dial :spit,avoiding => exhaust @08:05:37.36 SS.reconfigure SSfast @08:05:37.39 SS.seek 5.00ml @08:05:40.19 SSV.dial CBV @08:05:40.21
CBV.dial SSV @08:05:41.58 delay 3.8 seconds @08:05:45.46 SSV.dial :spit @08:05:46.81 SS.seek 0.00ml @08:05:49.44 SSV.dial CBV @08:05:50.80 CTV.dial :intake @08:05:51.57 Waiting for external sampler to finish priming... @08:05:51.58 delayUntil "08:15:37.14" @08:06:16.99 delay 1 second @08:06:18.56 AFV.select :holdingCoil @08:06:18.65 delay 1 second @08:06:19.66 ATV.dial :waste @08:06:20.68 AS.reconfigure ASnorm @08:06:20.71 AS.home.seek empty @08:07:08.79 ATV.dial :waste @08:07:08.80 ARV.dial between carrier and air1 @08:07:08.87 ASV.dial :waste1 @08:15:37.14 SSV.dial CBV @08:15:37.16
CBV.dial SSV @08:15:37.17 Allowing 55s for 7.0ml of remaining 7.0ml Intake.select :open @08:15:39.30 Intake @54.1psia, vacuum from 20.0 to 24.0psi @08:15:39.31 SS.reconfigure forSampling @08:15:39.35 SS.seek 7.00ml @08:15:45.93 Stroke completed -- waiting for pressure to equalize ... @08:15:46.57 Intake.select :close @08:15:46.70 SSV.dial :exhaust @08:15:47.48 Exhaust.select :open @08:15:47.57 SS.reconfigure SSexhale @08:15:47.61 SS.seek 0.00ml @08:15:51.18 Exhaust.select :close @08:15:51.28 Sampling 1000.0ml @08:15:51.29 Intake.select :close @08:15:51.30 Exhaust.select :close @08:15:51.31 SS.reconfigure SSconfig @08:15:51.34 SS.hold @08:15:51.49 CTV.dial :intake @08:15:51.50 SSV.dial CBV @08:15:51.52
CBV.dial SSV @08:15:52.30 Allowing 185s for 25.0ml of remaining 1000.0ml @08:15:52.31 Intake.select :open @08:15:54.46 Intake @53.9psia, vacuum from 20.0 to 24.0psi @08:15:54.47 SS.reconfigure forSampling @08:15:54.51 SS.seek 25.00ml @08:16:09.32 Stroke completed -- waiting for pressure to equalize ... @08:16:09.96 Intake.select :close @08:16:10.09 SSV.dial :exhaust @08:16:10.87 Exhaust.select :open @08:16:10.97 SS.reconfigure SSexhale @08:16:11.01 SS.seek 0.00ml @08:16:20.16 Exhaust.select :close @08:16:20.25 SSV.dial CBV @08:16:20.27
CBV.dial SSV @08:16:21.04 Allowing 185s for 25.0ml of remaining 975.0ml @08:16:21.05 Intake.select :open @08:16:23.18 Intake @53.9psia, vacuum from 20.0 to 24.0psi @08:16:23.19 SS.reconfigure forSampling @08:16:23.23 SS.seek 25.00ml @08:16:32.38 Vacuum too high -- waiting up to 40s @08:16:32.65 Intake @47.3psia, vacuum from 20.0 to 24.0psi @08:16:32.66 SS.reconfigure forSampling @08:16:32.70 SS.seek 25.00ml @08:16:39.21 Stroke completed -- waiting for pressure to equalize ... @08:16:39.85 Intake.select :close @08:16:39.98 SSV.dial :exhaust @08:16:40.77 Exhaust.select :open @08:16:40.86 SS.reconfigure SSexhale @08:16:40.90 SS.seek 0.00ml @08:16:49.96 Exhaust.select :close @08:16:50.05 SSV.dial CBV @08:16:50.07
CBV.dial SSV @08:16:50.85 Allowing 185s for 25.0ml of remaining 950.0ml @08:16:50.86 Intake.select :open @08:16:52.99 Intake @54.8psia, vacuum from 20.0 to 24.0psi @08:16:53.00 SS.reconfigure forSampling @08:16:53.04 SS.seek 25.00ml @08:16:57.13 Vacuum too high -- waiting up to 40s @08:16:57.38 Intake @48.2psia, vacuum from 20.0 to 24.0psi @08:16:57.39 SS.reconfigure forSampling @08:16:57.43 SS.seek 25.00ml @08:17:04.78 Vacuum too high -- waiting up to 40s @08:17:05.04 Intake @49.4psia, vacuum from 20.0 to 24.0psi @08:17:05.05 SS.reconfigure forSampling @08:17:05.09 SS.seek 25.00ml @08:17:06.95 Vacuum too high -- waiting up to 40s @08:17:07.20 Intake @49.6psia, vacuum from 20.0 to 24.0psi @08:17:07.21 SS.reconfigure forSampling @08:17:07.25 SS.seek 25.00ml @08:17:11.37 Vacuum too high -- waiting up to 40s @08:17:11.64 Intake @50.4psia, vacuum from 20.0 to 24.0psi @08:17:11.65 SS.reconfigure forSampling @08:17:11.68 SS.seek 25.00ml @08:17:15.14 Stroke completed -- waiting for pressure to equalize ... @08:17:15.77 Intake.select :close @08:17:15.91 SSV.dial :exhaust @08:17:16.69 Exhaust.select :open @08:17:16.78 SS.reconfigure SSexhale @08:17:16.82 SS.seek 0.00ml @08:17:25.77 Exhaust.select :close @08:17:25.86 SSV.dial CBV @08:17:25.88
CBV.dial SSV @08:17:26.66 Allowing 185s for 25.0ml of remaining 925.0ml @08:17:26.67 Intake.select :open @08:17:28.81 Intake @53.3psia, vacuum from 20.0 to 24.0psi @08:17:28.82 SS.reconfigure forSampling @08:17:28.85 SS.seek 25.00ml @08:17:31.77 Vacuum too high -- waiting up to 40s @08:17:32.03 Intake @51.9psia, vacuum from 20.0 to 24.0psi @08:17:32.04 SS.reconfigure forSampling @08:17:32.07 SS.seek 25.00ml @08:17:40.07 Vacuum too high -- waiting up to 40s @08:17:40.33 Intake @52.1psia, vacuum from 20.0 to 24.0psi @08:17:40.34 SS.reconfigure forSampling @08:17:40.38 SS.seek 25.00ml @08:17:56.38 Vacuum too high -- waiting up to 40s @08:17:56.64 Intake @53.2psia, vacuum from 20.0 to 24.0psi @08:17:56.65 SS.reconfigure forSampling @08:17:56.69 SS.seek 25.00ml @08:18:12.60 Stroke completed -- waiting for pressure to equalize ... @08:18:13.23 Intake.select :close @08:18:13.92 SSV.dial :exhaust @08:18:14.70 Exhaust.select :open @08:18:14.80 SS.reconfigure SSexhale @08:18:14.84 SS.seek 0.00ml @08:18:23.54 Exhaust.select :close @08:18:23.63 SSV.dial CBV @08:18:23.65
CBV.dial SSV @08:18:24.45 Allowing 185s for 25.0ml of remaining 900.0ml @08:18:24.46 Intake.select :open @08:18:26.59 Intake @53.8psia, vacuum from 20.0 to 24.0psi @08:18:26.60 SS.reconfigure forSampling @08:18:26.64 SS.seek 25.00ml @08:18:39.16 Vacuum too high -- waiting up to 40s @08:18:39.43 Intake @52.4psia, vacuum from 20.0 to 24.0psi @08:18:39.44 SS.reconfigure forSampling @08:18:39.47 SS.seek 25.00ml @08:20:07.27 Stroke completed -- waiting for pressure to equalize ... @08:20:07.91 Intake.select :close @08:20:08.04 SSV.dial :exhaust @08:20:08.85 Exhaust.select :open @08:20:08.95 SS.reconfigure SSexhale @08:20:08.99 SS.seek 0.00ml @08:20:17.77 Exhaust.select :close @08:20:17.86 SSV.dial CBV @08:20:17.88
CBV.dial SSV @08:20:18.66 Allowing 185s for 25.0ml of remaining 875.0ml @08:20:18.67 Intake.select :open @08:20:20.80 Intake @53.4psia, vacuum from 20.0 to 24.0psi @08:20:20.81 SS.reconfigure forSampling @08:20:20.85 SS.seek 25.00ml @08:21:19.00 Vacuum too high -- waiting up to 40s @08:21:19.27 Intake @52.1psia, vacuum from 20.0 to 24.0psi @08:21:19.28 SS.reconfigure forSampling @08:21:19.31 SS.seek 25.00ml @08:21:35.81 Vacuum too high -- waiting up to 40s @08:21:36.07 Intake @51.9psia, vacuum from 20.0 to 24.0psi @08:21:36.08 SS.reconfigure forSampling @08:21:36.12 SS.seek 25.00ml @08:21:43.31 Vacuum too high -- waiting up to 40s @08:21:43.57 Intake @52.2psia, vacuum from 20.0 to 24.0psi @08:21:43.58 SS.reconfigure forSampling @08:21:43.62 SS.seek 25.00ml @08:21:46.13 Vacuum too high -- waiting up to 40s @08:21:46.39 Intake @51.9psia, vacuum from 20.0 to 24.0psi @08:21:46.40 SS.reconfigure forSampling @08:21:46.43 SS.seek 25.00ml @08:21:50.78 Vacuum too high -- waiting up to 40s @08:21:51.04 Intake @52.5psia, vacuum from 20.0 to 24.0psi @08:21:51.05 SS.reconfigure forSampling @08:21:51.09 SS.seek 25.00ml @08:21:53.60 Vacuum too high -- waiting up to 40s @08:21:53.86 Intake @52.0psia, vacuum from 20.0 to 24.0psi @08:21:53.87 SS.reconfigure forSampling @08:21:53.90 SS.seek 25.00ml @08:21:55.47 Vacuum too high -- waiting up to 40s @08:21:55.72 Intake @51.5psia, vacuum from 20.0 to 24.0psi @08:21:55.73 SS.reconfigure forSampling @08:21:55.77 SS.seek 25.00ml @08:21:57.19 Vacuum too high -- waiting up to 40s @08:21:57.45 Intake @51.4psia, vacuum from 20.0 to 24.0psi @08:21:57.46 SS.reconfigure forSampling @08:21:57.49 SS.seek 25.00ml @08:21:59.32 Vacuum too high -- waiting up to 40s @08:21:59.57 Intake @51.1psia, vacuum from 20.0 to 24.0psi @08:21:59.58 SS.reconfigure forSampling @08:21:59.62 SS.seek 25.00ml @08:22:00.97 Vacuum too high -- waiting up to 40s @08:22:01.22 Intake @50.9psia, vacuum from 20.0 to 24.0psi @08:22:01.23 SS.reconfigure forSampling @08:22:01.27 SS.seek 25.00ml @08:22:07.18 Vacuum too high -- waiting up to 40s @08:22:07.44 Intake @51.4psia, vacuum from 20.0 to 24.0psi @08:22:07.45 SS.reconfigure forSampling @08:22:07.49 SS.seek 25.00ml @08:22:08.94 Vacuum too high -- waiting up to 40s @08:22:09.20 Intake @51.6psia, vacuum from 20.0 to 24.0psi @08:22:09.21 SS.reconfigure forSampling @08:22:09.25 SS.seek 25.00ml @08:22:10.75 Vacuum too high -- waiting up to 40s @08:22:11.00 Intake @51.7psia, vacuum from 20.0 to 24.0psi @08:22:11.01 SS.reconfigure forSampling @08:22:11.05 SS.seek 25.00ml @08:22:12.63 Vacuum too high -- waiting up to 40s @08:22:12.88 Intake @51.9psia, vacuum from 20.0 to 24.0psi @08:22:12.89 SS.reconfigure forSampling @08:22:12.93 SS.seek 25.00ml @08:22:14.35 Vacuum too high -- waiting up to 40s @08:22:14.60 Intake @51.8psia, vacuum from 20.0 to 24.0psi @08:22:14.61 SS.reconfigure forSampling @08:22:14.65 SS.seek 25.00ml @08:22:16.38 Vacuum too high -- waiting up to 40s @08:22:16.63 Intake @51.5psia, vacuum from 20.0 to 24.0psi @08:22:16.64 SS.reconfigure forSampling @08:22:16.68 SS.seek 25.00ml @08:22:18.00 Vacuum too high -- waiting up to 40s @08:22:18.25 Intake @51.2psia, vacuum from 20.0 to 24.0psi @08:22:18.26 SS.reconfigure forSampling @08:22:18.29 SS.seek 25.00ml @08:22:19.00 Stroke completed -- waiting for pressure to equalize ... @08:22:20.21 Intake.select :close @08:22:20.35 SSV.dial :exhaust @08:22:21.73 Exhaust.select :open @08:22:21.82 SS.reconfigure SSexhale @08:22:21.86 SS.seek 0.00ml @08:22:30.87 Exhaust.select :close @08:22:30.96 SSV.dial CBV @08:22:30.98
CBV.dial SSV @08:22:31.76 Allowing 185s for 25.0ml of remaining 850.0ml @08:22:31.77 Intake.select :open @08:22:33.90 Intake @51.8psia, vacuum from 20.0 to 24.0psi @08:22:33.91 SS.reconfigure forSampling @08:22:33.95 SS.seek 25.00ml @08:22:36.47 Vacuum too high -- waiting up to 40s @08:22:36.73 Intake @51.5psia, vacuum from 20.0 to 24.0psi @08:22:36.74 SS.reconfigure forSampling @08:22:36.77 SS.seek 25.00ml @08:22:38.53 Vacuum too high -- waiting up to 40s @08:22:38.79 Intake @51.3psia, vacuum from 20.0 to 24.0psi @08:22:38.80 SS.reconfigure forSampling @08:22:38.83 SS.seek 25.00ml @08:22:40.47 Vacuum too high -- waiting up to 40s @08:22:40.73 Intake @51.4psia, vacuum from 20.0 to 24.0psi @08:22:40.74 SS.reconfigure forSampling @08:22:40.77 SS.seek 25.00ml @08:22:43.22 Vacuum too high -- waiting up to 40s @08:22:43.48 Intake @51.9psia, vacuum from 20.0 to 24.0psi @08:22:43.49 SS.reconfigure forSampling @08:22:43.52 SS.seek 25.00ml @08:22:44.94 Vacuum too high -- waiting up to 40s @08:22:45.20 Intake @51.9psia, vacuum from 20.0 to 24.0psi @08:22:45.21 SS.reconfigure forSampling @08:22:45.24 SS.seek 25.00ml @08:22:46.41 Vacuum too high -- waiting up to 40s @08:22:46.67 Intake @51.6psia, vacuum from 20.0 to 24.0psi @08:22:46.68 SS.reconfigure forSampling @08:22:46.71 SS.seek 25.00ml @08:22:48.06 Vacuum too high -- waiting up to 40s @08:22:48.32 Intake @51.1psia, vacuum from 20.0 to 24.0psi @08:22:48.33 SS.reconfigure forSampling @08:22:48.36 SS.seek 25.00ml @08:22:49.69 Vacuum too high -- waiting up to 40s @08:22:49.94 Intake @51.0psia, vacuum from 20.0 to 24.0psi @08:22:49.95 SS.reconfigure forSampling @08:22:49.99 SS.seek 25.00ml @08:22:51.38 Vacuum too high -- waiting up to 40s @08:22:51.63 Intake @51.2psia, vacuum from 20.0 to 24.0psi @08:22:51.64 SS.reconfigure forSampling @08:22:51.68 SS.seek 25.00ml @08:22:52.94 Vacuum too high -- waiting up to 40s @08:22:53.20 Intake @51.2psia, vacuum from 20.0 to 24.0psi @08:22:53.21 SS.reconfigure forSampling @08:22:53.24 SS.seek 25.00ml @08:22:54.35 Vacuum too high -- waiting up to 40s @08:22:54.61 Intake @51.7psia, vacuum from 20.0 to 24.0psi @08:22:54.62 SS.reconfigure forSampling @08:22:54.65 SS.seek 25.00ml @08:22:55.66 Vacuum too high -- waiting up to 40s @08:22:55.92 Intake @51.5psia, vacuum from 20.0 to 24.0psi @08:22:55.93 SS.reconfigure forSampling @08:22:55.96 SS.seek 25.00ml @08:22:57.06 Vacuum too high -- waiting up to 40s @08:22:57.32 Intake @51.2psia, vacuum from 20.0 to 24.0psi @08:22:57.33 SS.reconfigure forSampling @08:22:57.36 SS.seek 25.00ml @08:22:58.59 Vacuum too high -- waiting up to 40s @08:22:58.85 Intake @51.3psia, vacuum from 20.0 to 24.0psi @08:22:58.86 SS.reconfigure forSampling @08:22:58.90 SS.seek 25.00ml @08:23:00.03 Vacuum too high -- waiting up to 40s @08:23:00.29 Intake @51.2psia, vacuum from 20.0 to 24.0psi @08:23:00.30 SS.reconfigure forSampling @08:23:00.33 SS.seek 25.00ml @08:23:01.84 Vacuum too high -- waiting up to 40s @08:23:02.09 Intake @51.9psia, vacuum from 20.0 to 24.0psi @08:23:02.10 SS.reconfigure forSampling @08:23:02.14 SS.seek 25.00ml @08:23:03.25 Vacuum too high -- waiting up to 40s @08:23:03.50 Intake @52.2psia, vacuum from 20.0 to 24.0psi @08:23:03.51 SS.reconfigure forSampling @08:23:03.55 SS.seek 25.00ml @08:23:04.84 Vacuum too high -- waiting up to 40s @08:23:05.09 Intake @52.5psia, vacuum from 20.0 to 24.0psi @08:23:05.10 SS.reconfigure forSampling @08:23:05.14 SS.seek 25.00ml @08:23:06.28 Vacuum too high -- waiting up to 40s @08:23:06.53 Intake @52.1psia, vacuum from 20.0 to 24.0psi @08:23:06.54 SS.reconfigure forSampling @08:23:06.58 SS.seek 25.00ml @08:23:07.75 Vacuum too high -- waiting up to 40s @08:23:08.01 Intake @51.8psia, vacuum from 20.0 to 24.0psi @08:23:08.02 SS.reconfigure forSampling @08:23:08.05 SS.seek 25.00ml @08:23:09.19 Vacuum too high -- waiting up to 40s @08:23:09.45 Intake @51.1psia, vacuum from 20.0 to 24.0psi @08:23:09.46 SS.reconfigure forSampling @08:23:09.49 SS.seek 25.00ml @08:23:10.50 Vacuum too high -- waiting up to 40s @08:23:10.76 Intake @51.0psia, vacuum from 20.0 to 24.0psi @08:23:10.77 SS.reconfigure forSampling @08:23:10.80 SS.seek 25.00ml @08:23:12.43 Vacuum too high -- waiting up to 40s @08:23:12.71 SS.seek 25.00ml @08:23:14.75 Vacuum too high -- waiting up to 40s @08:23:15.01 Intake @51.3psia, vacuum from 20.0 to 24.0psi @08:23:15.02 SS.reconfigure forSampling @08:23:15.06 SS.seek 25.00ml @08:23:16.41 Vacuum too high -- waiting up to 40s @08:23:16.67 Intake @51.4psia, vacuum from 20.0 to 24.0psi @08:23:16.68 SS.reconfigure forSampling @08:23:16.72 SS.seek 25.00ml @08:23:17.66 Vacuum too high -- waiting up to 40s @08:23:17.92 Intake @51.4psia, vacuum from 20.0 to 24.0psi @08:23:17.93 SS.reconfigure forSampling @08:23:17.96 SS.seek 25.00ml @08:23:18.97 Vacuum too high -- waiting up to 40s @08:23:19.24 Intake @51.6psia, vacuum from 20.0 to 24.0psi @08:23:19.25 SS.reconfigure forSampling @08:23:19.28 SS.seek 25.00ml @08:23:20.28 Vacuum too high -- waiting up to 40s @08:23:20.54 Intake @51.7psia, vacuum from 20.0 to 24.0psi @08:23:20.56 SS.reconfigure forSampling @08:23:20.59 SS.seek 25.00ml @08:23:21.50 Vacuum too high -- waiting up to 40s @08:23:21.76 Intake @51.9psia, vacuum from 20.0 to 24.0psi @08:23:21.77 SS.reconfigure forSampling @08:23:21.80 SS.seek 25.00ml @08:23:22.66 Vacuum too high -- waiting up to 40s @08:23:22.92 Intake @51.6psia, vacuum from 20.0 to 24.0psi @08:23:22.93 SS.reconfigure forSampling @08:23:22.96 SS.seek 25.00ml @08:23:23.78 Vacuum too high -- waiting up to 40s @08:23:24.04 Intake @51.3psia, vacuum from 20.0 to 24.0psi @08:23:24.05 SS.reconfigure forSampling @08:23:24.09 SS.seek 25.00ml @08:23:25.00 Vacuum too high -- waiting up to 40s @08:23:25.26 Intake @50.9psia, vacuum from 20.0 to 24.0psi @08:23:25.27 SS.reconfigure forSampling @08:23:25.30 SS.seek 25.00ml @08:23:26.34 Vacuum too high -- waiting up to 40s @08:23:26.60 Intake @50.8psia, vacuum from 20.0 to 24.0psi @08:23:26.61 SS.reconfigure forSampling @08:23:26.64 SS.seek 25.00ml @08:23:27.66 Vacuum too high -- waiting up to 40s @08:23:27.92 Intake @50.9psia, vacuum from 20.0 to 24.0psi @08:23:27.93 SS.reconfigure forSampling @08:23:27.96 SS.seek 25.00ml @08:23:28.91 Vacuum too high -- waiting up to 40s @08:23:29.17 Intake @51.1psia, vacuum from 20.0 to 24.0psi @08:23:29.18 SS.reconfigure forSampling @08:23:29.21 SS.seek 25.00ml @08:23:30.28 Vacuum too high -- waiting up to 40s @08:23:30.54 Intake @51.4psia, vacuum from 20.0 to 24.0psi @08:23:30.55 SS.reconfigure forSampling @08:23:30.58 SS.seek 25.00ml @08:23:31.50 Vacuum too high -- waiting up to 40s @08:23:31.76 Intake @51.6psia, vacuum from 20.0 to 24.0psi @08:23:31.77 SS.reconfigure forSampling @08:23:31.80 SS.seek 25.00ml @08:23:32.62 Vacuum too high -- waiting up to 40s @08:23:32.88 Intake @51.9psia, vacuum from 20.0 to 24.0psi @08:23:32.89 SS.reconfigure forSampling @08:23:32.93 SS.seek 25.00ml @08:23:33.78 Vacuum too high -- waiting up to 40s @08:23:34.04 Intake @51.5psia, vacuum from 20.0 to 24.0psi @08:23:34.05 SS.reconfigure forSampling @08:23:34.08 SS.seek 25.00ml @08:23:35.00 Vacuum too high -- waiting up to 40s @08:23:35.26 Intake @51.1psia, vacuum from 20.0 to 24.0psi @08:23:35.27 SS.reconfigure forSampling @08:23:35.30 SS.seek 25.00ml @08:23:36.12 Vacuum too high -- waiting up to 40s @08:23:36.38 SS.seek 25.00ml @08:23:37.19 Vacuum too high -- waiting up to 40s @08:23:37.45 Intake @51.2psia, vacuum from 20.0 to 24.0psi @08:23:37.46 SS.reconfigure forSampling @08:23:37.49 SS.seek 25.00ml @08:23:38.37 Vacuum too high -- waiting up to 40s @08:23:38.63 Intake @51.4psia, vacuum from 20.0 to 24.0psi @08:23:38.64 SS.reconfigure forSampling @08:23:38.67 SS.seek 25.00ml @08:23:39.53 Vacuum too high -- waiting up to 40s @08:23:39.79 Intake @52.0psia, vacuum from 20.0 to 24.0psi @08:23:39.80 SS.reconfigure forSampling @08:23:39.84 SS.seek 25.00ml @08:23:40.52 Vacuum too high -- waiting up to 40s @08:23:40.79 Intake @51.8psia, vacuum from 20.0 to 24.0psi @08:23:40.80 SS.reconfigure forSampling @08:23:40.83 SS.seek 25.00ml @08:23:41.72 Vacuum too high -- waiting up to 40s @08:23:41.98 Intake @51.5psia, vacuum from 20.0 to 24.0psi @08:23:41.99 SS.reconfigure forSampling @08:23:42.02 SS.seek 25.00ml @08:23:42.97 Vacuum too high -- waiting up to 40s @08:23:43.23 Intake @51.2psia, vacuum from 20.0 to 24.0psi @08:23:43.87 SS.reconfigure forSampling @08:23:43.91 SS.seek 25.00ml @08:23:45.50 Vacuum too high -- waiting up to 40s @08:23:45.76 Intake @51.4psia, vacuum from 20.0 to 24.0psi @08:23:45.77 SS.reconfigure forSampling @08:23:45.80 SS.seek 25.00ml @08:23:46.56 Vacuum too high -- waiting up to 40s @08:23:46.82 Intake @51.3psia, vacuum from 20.0 to 24.0psi @08:23:46.83 SS.reconfigure forSampling @08:23:46.86 SS.seek 25.00ml @08:23:47.87 Vacuum too high -- waiting up to 40s @08:23:48.13 Intake @51.5psia, vacuum from 20.0 to 24.0psi @08:23:48.14 SS.reconfigure forSampling @08:23:48.18 SS.seek 25.00ml @08:23:48.97 Vacuum too high -- waiting up to 40s @08:23:49.23 Intake @51.1psia, vacuum from 20.0 to 24.0psi @08:23:49.24 SS.reconfigure forSampling @08:23:49.27 SS.seek 25.00ml @08:23:50.31 Vacuum too high -- waiting up to 40s @08:23:50.57 Intake @51.2psia, vacuum from 20.0 to 24.0psi @08:23:50.58 SS.reconfigure forSampling @08:23:50.62 SS.seek 25.00ml @08:23:51.47 Vacuum too high -- waiting up to 40s @08:23:51.73 Intake @51.3psia, vacuum from 20.0 to 24.0psi @08:23:51.74 SS.reconfigure forSampling @08:23:51.77 SS.seek 25.00ml @08:23:52.59 Vacuum too high -- waiting up to 40s @08:23:52.85 Intake @51.5psia, vacuum from 20.0 to 24.0psi @08:23:52.86 SS.reconfigure forSampling @08:23:52.89 SS.seek 25.00ml @08:23:53.78 Vacuum too high -- waiting up to 40s @08:23:54.04 Intake @51.8psia, vacuum from 20.0 to 24.0psi @08:23:54.05 SS.reconfigure forSampling @08:23:54.09 SS.seek 25.00ml @08:23:54.84 Vacuum too high -- waiting up to 40s @08:23:55.10 Intake @52.0psia, vacuum from 20.0 to 24.0psi @08:23:55.11 SS.reconfigure forSampling @08:23:55.14 SS.seek 25.00ml @08:23:55.87 Vacuum too high -- waiting up to 40s @08:23:56.13 Intake @52.3psia, vacuum from 20.0 to 24.0psi @08:23:56.14 SS.reconfigure forSampling @08:23:56.17 SS.seek 25.00ml @08:23:56.90 Vacuum too high -- waiting up to 40s @08:23:57.16 Intake @52.4psia, vacuum from 20.0 to 24.0psi @08:23:57.17 SS.reconfigure forSampling @08:23:57.20 SS.seek 25.00ml @08:23:58.00 Vacuum too high -- waiting up to 40s @08:23:58.27 SS.seek 25.00ml @08:23:59.00 Vacuum too high -- waiting up to 40s @08:23:59.26 Intake @52.2psia, vacuum from 20.0 to 24.0psi @08:23:59.27 SS.reconfigure forSampling @08:23:59.30 SS.seek 25.00ml @08:24:00.19 Vacuum too high -- waiting up to 40s @08:24:00.44 Intake @51.6psia, vacuum from 20.0 to 24.0psi @08:24:00.45 SS.reconfigure forSampling @08:24:00.49 SS.seek 25.00ml @08:24:01.31 Vacuum too high -- waiting up to 40s @08:24:01.58 SS.seek 25.00ml @08:24:02.31 Vacuum too high -- waiting up to 40s @08:24:02.58 Intake @51.7psia, vacuum from 20.0 to 24.0psi @08:24:02.59 SS.reconfigure forSampling @08:24:02.62 SS.seek 25.00ml @08:24:03.34 Vacuum too high -- waiting up to 40s @08:24:03.60 Intake @51.6psia, vacuum from 20.0 to 24.0psi @08:24:03.61 SS.reconfigure forSampling @08:24:03.64 SS.seek 25.00ml @08:24:04.37 Vacuum too high -- waiting up to 40s @08:24:04.63 Intake @51.7psia, vacuum from 20.0 to 24.0psi @08:24:04.64 SS.reconfigure forSampling @08:24:04.67 SS.seek 25.00ml @08:24:05.44 Vacuum too high -- waiting up to 40s @08:24:05.70 Intake @51.4psia, vacuum from 20.0 to 24.0psi @08:24:05.71 SS.reconfigure forSampling @08:24:05.74 SS.seek 25.00ml @08:24:06.50 Vacuum too high -- waiting up to 40s @08:24:06.76 Intake @51.4psia, vacuum from 20.0 to 24.0psi @08:24:06.77 SS.reconfigure forSampling @08:24:06.80 SS.seek 25.00ml @08:24:07.62 Vacuum too high -- waiting up to 40s @08:24:07.88 Intake @51.2psia, vacuum from 20.0 to 24.0psi @08:24:07.89 SS.reconfigure forSampling @08:24:07.93 SS.seek 25.00ml @08:24:08.77 Vacuum too high -- waiting up to 40s @08:24:09.04 Intake @51.5psia, vacuum from 20.0 to 24.0psi @08:24:09.05 SS.reconfigure forSampling @08:24:09.08 SS.seek 25.00ml @08:24:09.87 Vacuum too high -- waiting up to 40s @08:24:10.13 Intake @52.1psia, vacuum from 20.0 to 24.0psi @08:24:10.14 SS.reconfigure forSampling @08:24:10.18 SS.seek 25.00ml @08:24:11.06 Vacuum too high -- waiting up to 40s @08:24:11.31 Intake @52.9psia, vacuum from 20.0 to 24.0psi @08:24:11.32 SS.reconfigure forSampling @08:24:11.36 SS.seek 25.00ml @08:24:12.70 Vacuum too high -- waiting up to 40s @08:24:12.97 Intake @51.9psia, vacuum from 20.0 to 24.0psi @08:24:12.98 SS.reconfigure forSampling @08:24:13.01 SS.seek 25.00ml @08:24:14.39 Vacuum too high -- waiting up to 40s @08:24:14.66 Intake @51.1psia, vacuum from 20.0 to 24.0psi @08:24:14.67 SS.reconfigure forSampling @08:24:14.70 SS.seek 25.00ml @08:24:15.43 Vacuum too high -- waiting up to 40s @08:24:15.69 Intake @50.8psia, vacuum from 20.0 to 24.0psi @08:24:15.70 SS.reconfigure forSampling @08:24:15.74 SS.seek 25.00ml @08:24:16.53 Vacuum too high -- waiting up to 40s @08:24:16.79 Intake @50.8psia, vacuum from 20.0 to 24.0psi @08:24:16.80 SS.reconfigure forSampling @08:24:16.83 SS.seek 25.00ml @08:24:17.72 Vacuum too high -- waiting up to 40s @08:24:17.98 Intake @50.9psia, vacuum from 20.0 to 24.0psi @08:24:17.99 SS.reconfigure forSampling @08:24:18.02 SS.seek 25.00ml @08:24:18.81 Vacuum too high -- waiting up to 40s @08:24:19.09 Intake @51.4psia, vacuum from 20.0 to 24.0psi @08:24:19.10 SS.reconfigure forSampling @08:24:19.13 SS.seek 25.00ml @08:24:19.93 Vacuum too high -- waiting up to 40s @08:24:20.19 Intake @51.8psia, vacuum from 20.0 to 24.0psi @08:24:20.20 SS.reconfigure forSampling @08:24:20.23 SS.seek 25.00ml @08:24:21.05 Vacuum too high -- waiting up to 40s @08:24:21.32 Intake @51.6psia, vacuum from 20.0 to 24.0psi @08:24:21.33 SS.reconfigure forSampling @08:24:21.36 SS.seek 25.00ml @08:24:22.06 Vacuum too high -- waiting up to 40s @08:24:22.32 Intake @51.0psia, vacuum from 20.0 to 24.0psi @08:24:22.33 SS.reconfigure forSampling @08:24:22.36 SS.seek 25.00ml @08:24:23.18 Vacuum too high -- waiting up to 40s @08:24:23.44 Intake @50.9psia, vacuum from 20.0 to 24.0psi @08:24:23.45 SS.reconfigure forSampling @08:24:23.48 SS.seek 25.00ml @08:24:24.25 Vacuum too high -- waiting up to 40s @08:24:24.50 Intake @50.8psia, vacuum from 20.0 to 24.0psi @08:24:24.52 SS.reconfigure forSampling @08:24:24.55 SS.seek 25.00ml @08:24:25.25 Vacuum too high -- waiting up to 40s @08:24:25.51 Intake @50.8psia, vacuum from 20.0 to 24.0psi @08:24:25.52 SS.reconfigure forSampling @08:24:25.55 SS.seek 25.00ml @08:24:26.28 Vacuum too high -- waiting up to 40s @08:24:26.54 Intake @50.9psia, vacuum from 20.0 to 24.0psi @08:24:26.55 SS.reconfigure forSampling @08:24:26.58 SS.seek 25.00ml @08:24:27.31 Vacuum too high -- waiting up to 40s @08:24:27.57 Intake @51.4psia, vacuum from 20.0 to 24.0psi @08:24:27.58 SS.reconfigure forSampling @08:24:27.61 SS.seek 25.00ml @08:24:28.28 Vacuum too high -- waiting up to 40s @08:24:28.54 Intake @51.7psia, vacuum from 20.0 to 24.0psi @08:24:28.55 SS.reconfigure forSampling @08:24:28.58 SS.seek 25.00ml @08:24:29.22 Vacuum too high -- waiting up to 40s @08:24:29.48 Intake @51.8psia, vacuum from 20.0 to 24.0psi @08:24:29.49 SS.reconfigure forSampling @08:24:29.52 SS.seek 25.00ml @08:24:30.40 Vacuum too high -- waiting up to 40s @08:24:30.66 Intake @51.9psia, vacuum from 20.0 to 24.0psi @08:24:30.67 SS.reconfigure forSampling @08:24:30.70 SS.seek 25.00ml @08:24:31.53 Vacuum too high -- waiting up to 40s @08:24:31.78 Intake @51.1psia, vacuum from 20.0 to 24.0psi @08:24:31.79 SS.reconfigure forSampling @08:24:31.83 SS.seek 25.00ml @08:24:32.65 Vacuum too high -- waiting up to 40s @08:24:32.91 Intake @50.9psia, vacuum from 20.0 to 24.0psi @08:24:32.92 SS.reconfigure forSampling @08:24:32.95 SS.seek 25.00ml @08:24:33.75 Vacuum too high -- waiting up to 40s @08:24:34.01 Intake @50.8psia, vacuum from 20.0 to 24.0psi @08:24:34.02 SS.reconfigure forSampling @08:24:34.06 SS.seek 25.00ml @08:24:34.75 Vacuum too high -- waiting up to 40s @08:24:35.14 Intake @51.1psia, vacuum from 20.0 to 24.0psi @08:24:35.15 SS.reconfigure forSampling @08:24:35.18 SS.seek 25.00ml @08:24:36.09 Vacuum too high -- waiting up to 40s @08:24:36.35 Intake @50.9psia, vacuum from 20.0 to 24.0psi @08:24:36.36 SS.reconfigure forSampling @08:24:36.39 SS.seek 25.00ml @08:24:37.15 Vacuum too high -- waiting up to 40s @08:24:37.40 Intake @51.1psia, vacuum from 20.0 to 24.0psi @08:24:37.41 SS.reconfigure forSampling @08:24:37.45 SS.seek 25.00ml @08:24:38.18 Vacuum too high -- waiting up to 40s @08:24:38.44 Intake @51.2psia, vacuum from 20.0 to 24.0psi @08:24:38.45 SS.reconfigure forSampling @08:24:38.49 SS.seek 25.00ml @08:24:39.21 Vacuum too high -- waiting up to 40s @08:24:39.98 Intake @51.3psia, vacuum from 20.0 to 24.0psi @08:24:39.99 SS.reconfigure forSampling @08:24:40.03 SS.seek 25.00ml @08:24:41.31 Vacuum too high -- waiting up to 40s @08:24:41.57 Intake @50.9psia, vacuum from 20.0 to 24.0psi @08:24:41.58 SS.reconfigure forSampling @08:24:41.62 SS.seek 25.00ml @08:24:42.34 Vacuum too high -- waiting up to 40s @08:24:42.60 Intake @50.6psia, vacuum from 20.0 to 24.0psi @08:24:42.61 SS.reconfigure forSampling @08:24:42.64 SS.seek 25.00ml @08:24:43.37 Vacuum too high -- waiting up to 40s @08:24:43.63 Intake @50.6psia, vacuum from 20.0 to 24.0psi @08:24:43.64 SS.reconfigure forSampling @08:24:43.68 SS.seek 25.00ml @08:24:44.40 Vacuum too high -- waiting up to 40s @08:24:44.67 Intake @50.5psia, vacuum from 20.0 to 24.0psi @08:24:44.68 SS.reconfigure forSampling @08:24:44.71 SS.seek 25.00ml @08:24:45.40 Vacuum too high -- waiting up to 40s @08:24:45.66 Intake @50.6psia, vacuum from 20.0 to 24.0psi @08:24:45.67 SS.reconfigure forSampling @08:24:45.70 SS.seek 25.00ml @08:24:46.40 Vacuum too high -- waiting up to 40s @08:24:46.66 Intake @51.1psia, vacuum from 20.0 to 24.0psi @08:24:46.67 SS.reconfigure forSampling @08:24:46.71 SS.seek 25.00ml @08:24:47.34 Vacuum too high -- waiting up to 40s @08:24:47.60 Intake @51.7psia, vacuum from 20.0 to 24.0psi @08:24:47.61 SS.reconfigure forSampling @08:24:47.65 SS.seek 25.00ml @08:24:48.25 Vacuum too high -- waiting up to 40s @08:24:48.51 Intake @51.9psia, vacuum from 20.0 to 24.0psi @08:24:48.52 SS.reconfigure forSampling @08:24:48.56 SS.seek 25.00ml @08:24:49.34 Vacuum too high -- waiting up to 40s @08:24:49.60 Intake @50.8psia, vacuum from 20.0 to 24.0psi @08:24:49.61 SS.reconfigure forSampling @08:24:49.64 SS.seek 25.00ml @08:24:50.34 Vacuum too high -- waiting up to 40s @08:24:50.60 Intake @50.5psia, vacuum from 20.0 to 24.0psi @08:24:50.61 SS.reconfigure forSampling @08:24:50.64 SS.seek 25.00ml @08:24:51.40 Vacuum too high -- waiting up to 40s @08:24:51.66 Intake @50.3psia, vacuum from 20.0 to 24.0psi @08:24:51.67 SS.reconfigure forSampling @08:24:51.70 SS.seek 25.00ml @08:24:52.46 Vacuum too high -- waiting up to 40s @08:24:52.72 Intake @50.5psia, vacuum from 20.0 to 24.0psi @08:24:52.73 SS.reconfigure forSampling @08:24:52.77 SS.seek 25.00ml @08:24:53.43 Vacuum too high -- waiting up to 40s @08:24:53.69 SS.seek 25.00ml @08:24:54.25 Vacuum too high -- waiting up to 40s @08:24:54.51 Intake @50.6psia, vacuum from 20.0 to 24.0psi @08:24:54.52 SS.reconfigure forSampling @08:24:54.55 SS.seek 25.00ml @08:24:55.28 Vacuum too high -- waiting up to 40s @08:24:55.54 Intake @51.2psia, vacuum from 20.0 to 24.0psi @08:24:55.55 SS.reconfigure forSampling @08:24:55.58 SS.seek 25.00ml @08:24:56.25 Vacuum too high -- waiting up to 40s @08:24:56.51 Intake @51.6psia, vacuum from 20.0 to 24.0psi @08:24:56.52 SS.reconfigure forSampling @08:24:56.55 SS.seek 25.00ml @08:24:57.21 Vacuum too high -- waiting up to 40s @08:24:57.47 Intake @51.5psia, vacuum from 20.0 to 24.0psi @08:24:57.48 SS.reconfigure forSampling @08:24:57.51 SS.seek 25.00ml @08:24:58.18 Vacuum too high -- waiting up to 40s @08:24:58.43 Intake @51.2psia, vacuum from 20.0 to 24.0psi @08:24:58.45 SS.reconfigure forSampling @08:24:58.48 SS.seek 25.00ml @08:24:59.15 Vacuum too high -- waiting up to 40s @08:24:59.41 Intake @50.9psia, vacuum from 20.0 to 24.0psi @08:24:59.42 SS.reconfigure forSampling @08:24:59.45 SS.seek 25.00ml @08:25:00.15 Vacuum too high -- waiting up to 40s @08:25:00.41 Intake @50.8psia, vacuum from 20.0 to 24.0psi @08:25:00.42 SS.reconfigure forSampling @08:25:00.45 SS.seek 25.00ml @08:25:01.06 Vacuum too high -- waiting up to 40s @08:25:01.33 SS.seek 25.00ml @08:25:01.99 Vacuum too high -- waiting up to 40s @08:25:02.47 Intake @51.2psia, vacuum from 20.0 to 24.0psi @08:25:02.48 SS.reconfigure forSampling @08:25:02.51 SS.seek 25.00ml @08:25:03.34 Vacuum too high -- waiting up to 40s @08:25:03.59 Intake @51.5psia, vacuum from 20.0 to 24.0psi @08:25:03.60 SS.reconfigure forSampling @08:25:03.64 SS.seek 25.00ml @08:25:04.24 Vacuum too high -- waiting up to 40s @08:25:05.14 Intake @51.4psia, vacuum from 20.0 to 24.0psi @08:25:05.15 SS.reconfigure forSampling @08:25:05.18 SS.seek 25.00ml @08:25:06.40 Vacuum too high -- waiting up to 40s @08:25:06.66 Intake @51.1psia, vacuum from 20.0 to 24.0psi @08:25:06.67 SS.reconfigure forSampling @08:25:06.70 SS.seek 25.00ml @08:25:07.46 Vacuum too high -- waiting up to 40s @08:25:07.72 Intake @51.0psia, vacuum from 20.0 to 24.0psi @08:25:07.73 SS.reconfigure forSampling @08:25:07.76 SS.seek 25.00ml @08:25:08.49 Vacuum too high -- waiting up to 40s @08:25:08.76 SS.seek 25.00ml @08:25:09.43 Vacuum too high -- waiting up to 40s @08:25:09.69 Intake @50.8psia, vacuum from 20.0 to 24.0psi @08:25:09.70 SS.reconfigure forSampling @08:25:09.74 SS.seek 25.00ml @08:25:10.43 Vacuum too high -- waiting up to 40s @08:25:10.91 Intake @51.4psia, vacuum from 20.0 to 24.0psi @08:25:10.92 SS.reconfigure forSampling @08:25:10.95 SS.seek 25.00ml @08:25:11.87 Vacuum too high -- waiting up to 40s @08:25:12.13 Intake @51.9psia, vacuum from 20.0 to 24.0psi @08:25:12.14 SS.reconfigure forSampling @08:25:12.17 SS.seek 25.00ml @08:25:12.74 Vacuum too high -- waiting up to 40s @08:25:13.00 Intake @51.9psia, vacuum from 20.0 to 24.0psi @08:25:13.01 SS.reconfigure forSampling @08:25:13.05 SS.seek 25.00ml @08:25:13.71 Vacuum too high -- waiting up to 40s @08:25:13.97 Intake @51.6psia, vacuum from 20.0 to 24.0psi @08:25:13.98 SS.reconfigure forSampling @08:25:14.01 SS.seek 25.00ml @08:25:14.62 Vacuum too high -- waiting up to 40s @08:25:14.88 Intake @51.1psia, vacuum from 20.0 to 24.0psi @08:25:14.89 SS.reconfigure forSampling @08:25:14.92 SS.seek 25.00ml @08:25:15.62 Vacuum too high -- waiting up to 40s @08:25:15.88 Intake @51.0psia, vacuum from 20.0 to 24.0psi @08:25:15.89 SS.reconfigure forSampling @08:25:15.92 SS.seek 25.00ml @08:25:16.56 Vacuum too high -- waiting up to 40s @08:25:16.82 Intake @50.9psia, vacuum from 20.0 to 24.0psi @08:25:16.83 SS.reconfigure forSampling @08:25:16.86 SS.seek 25.00ml @08:25:17.53 Vacuum too high -- waiting up to 40s @08:25:17.79 Intake @50.8psia, vacuum from 20.0 to 24.0psi @08:25:17.80 SS.reconfigure forSampling @08:25:17.83 SS.seek 25.00ml @08:25:18.46 Vacuum too high -- waiting up to 40s @08:25:18.72 Intake @50.7psia, vacuum from 20.0 to 24.0psi @08:25:18.73 SS.reconfigure forSampling @08:25:18.76 SS.seek 25.00ml @08:25:19.43 Vacuum too high -- waiting up to 40s @08:25:19.69 Intake @50.4psia, vacuum from 20.0 to 24.0psi @08:25:19.70 SS.reconfigure forSampling @08:25:19.73 SS.seek 25.00ml @08:25:20.34 Vacuum too high -- waiting up to 40s @08:25:20.60 Intake @50.5psia, vacuum from 20.0 to 24.0psi @08:25:20.61 SS.reconfigure forSampling @08:25:20.64 SS.seek 25.00ml @08:25:21.24 Vacuum too high -- waiting up to 40s @08:25:21.51 SS.seek 25.00ml @08:25:22.15 Vacuum too high -- waiting up to 40s @08:25:22.41 Intake @50.7psia, vacuum from 20.0 to 24.0psi @08:25:22.42 SS.reconfigure forSampling @08:25:22.45 SS.seek 25.00ml @08:25:23.21 Vacuum too high -- waiting up to 40s @08:25:23.68 Intake @52.0psia, vacuum from 20.0 to 24.0psi @08:25:23.69 SS.reconfigure forSampling @08:25:23.72 SS.seek 25.00ml @08:25:24.46 Vacuum too high -- waiting up to 40s @08:25:24.72 Intake @52.1psia, vacuum from 20.0 to 24.0psi @08:25:24.73 SS.reconfigure forSampling @08:25:24.76 SS.seek 25.00ml @08:25:25.40 Vacuum too high -- waiting up to 40s @08:25:25.66 Intake @51.4psia, vacuum from 20.0 to 24.0psi @08:25:25.67 SS.reconfigure forSampling @08:25:25.70 SS.seek 25.00ml @08:25:26.31 Vacuum too high -- waiting up to 40s @08:25:26.57 Intake @50.8psia, vacuum from 20.0 to 24.0psi @08:25:26.58 SS.reconfigure forSampling @08:25:26.61 SS.seek 25.00ml @08:25:27.18 Vacuum too high -- waiting up to 40s @08:25:27.44 Intake @50.6psia, vacuum from 20.0 to 24.0psi @08:25:27.45 SS.reconfigure forSampling @08:25:27.48 SS.seek 25.00ml @08:25:28.06 Vacuum too high -- waiting up to 40s @08:25:28.31 Intake @50.5psia, vacuum from 20.0 to 24.0psi @08:25:28.32 SS.reconfigure forSampling @08:25:28.36 SS.seek 25.00ml @08:25:29.00 Vacuum too high -- waiting up to 40s @08:25:29.89 Intake @50.6psia, vacuum from 20.0 to 24.0psi @08:25:29.90 SS.reconfigure forSampling @08:25:29.93 SS.seek 25.00ml @08:25:31.09 Vacuum too high -- waiting up to 40s @08:25:31.35 Intake @50.8psia, vacuum from 20.0 to 24.0psi @08:25:31.36 SS.reconfigure forSampling @08:25:31.40 SS.seek 25.00ml @08:25:32.09 Vacuum too high -- waiting up to 40s @08:25:32.58 Intake @51.2psia, vacuum from 20.0 to 24.0psi @08:25:32.59 SS.reconfigure forSampling @08:25:32.62 SS.seek 25.00ml @08:25:33.33 Stroke completed -- waiting for pressure to equalize ... @08:25:35.73 Intake.select :close @08:25:35.86 SSV.dial :exhaust @08:25:36.64 Exhaust.select :open @08:25:36.73 SS.reconfigure SSexhale @08:25:36.77 SS.seek 0.00ml @08:25:45.99 Exhaust.select :close @08:25:46.08 SSV.dial CBV @08:25:46.10
CBV.dial SSV @08:25:46.89 Allowing 185s for 25.0ml of remaining 825.0ml @08:25:46.90 Intake.select :open @08:25:49.03 Intake @50.8psia, vacuum from 20.0 to 24.0psi @08:25:49.04 SS.reconfigure forSampling @08:25:49.08 SS.seek 25.00ml @08:25:50.65 Vacuum too high -- waiting up to 40s @08:25:50.90 Intake @51.4psia, vacuum from 20.0 to 24.0psi @08:25:50.91 SS.reconfigure forSampling @08:25:50.95 SS.seek 25.00ml @08:25:51.71 Vacuum too high -- waiting up to 40s @08:25:51.97 Intake @51.8psia, vacuum from 20.0 to 24.0psi @08:25:51.98 SS.reconfigure forSampling @08:25:52.01 SS.seek 25.00ml @08:25:52.65 Vacuum too high -- waiting up to 40s @08:25:52.91 Intake @51.8psia, vacuum from 20.0 to 24.0psi @08:25:52.92 SS.reconfigure forSampling @08:25:52.95 SS.seek 25.00ml @08:25:53.59 Vacuum too high -- waiting up to 40s @08:25:53.86 Intake @51.2psia, vacuum from 20.0 to 24.0psi @08:25:53.87 SS.reconfigure forSampling @08:25:53.90 SS.seek 25.00ml @08:25:54.65 Vacuum too high -- waiting up to 40s @08:25:54.91 Intake @50.9psia, vacuum from 20.0 to 24.0psi @08:25:54.92 SS.reconfigure forSampling @08:25:54.95 SS.seek 25.00ml @08:25:55.74 Vacuum too high -- waiting up to 40s @08:25:56.21 Intake @50.9psia, vacuum from 20.0 to 24.0psi @08:25:56.22 SS.reconfigure forSampling @08:25:56.25 SS.seek 25.00ml @08:25:57.27 Vacuum too high -- waiting up to 40s @08:25:57.53 Intake @50.9psia, vacuum from 20.0 to 24.0psi @08:25:57.54 SS.reconfigure forSampling @08:25:57.58 SS.seek 25.00ml @08:25:58.18 Vacuum too high -- waiting up to 40s @08:25:58.44 Intake @50.9psia, vacuum from 20.0 to 24.0psi @08:25:58.45 SS.reconfigure forSampling @08:25:58.48 SS.seek 25.00ml @08:25:59.12 Vacuum too high -- waiting up to 40s @08:25:59.37 Intake @50.8psia, vacuum from 20.0 to 24.0psi @08:25:59.38 SS.reconfigure forSampling @08:25:59.42 SS.seek 25.00ml @08:26:00.02 Vacuum too high -- waiting up to 40s @08:26:00.28 Intake @50.9psia, vacuum from 20.0 to 24.0psi @08:26:00.29 SS.reconfigure forSampling @08:26:00.32 SS.seek 25.00ml @08:26:01.05 Vacuum too high -- waiting up to 40s @08:26:01.31 Intake @51.4psia, vacuum from 20.0 to 24.0psi @08:26:01.32 SS.reconfigure forSampling @08:26:01.35 SS.seek 25.00ml @08:26:02.02 Vacuum too high -- waiting up to 40s @08:26:02.49 Intake @51.7psia, vacuum from 20.0 to 24.0psi @08:26:02.50 SS.reconfigure forSampling @08:26:02.53 SS.seek 25.00ml @08:26:03.49 Vacuum too high -- waiting up to 40s @08:26:03.74 Intake @52.0psia, vacuum from 20.0 to 24.0psi @08:26:03.75 SS.reconfigure forSampling @08:26:03.79 SS.seek 25.00ml @08:26:04.59 Vacuum too high -- waiting up to 40s @08:26:04.85 Intake @52.1psia, vacuum from 20.0 to 24.0psi @08:26:04.86 SS.reconfigure forSampling @08:26:04.89 SS.seek 25.00ml @08:26:05.46 Vacuum too high -- waiting up to 40s @08:26:05.71 Intake @51.8psia, vacuum from 20.0 to 24.0psi @08:26:05.72 SS.reconfigure forSampling @08:26:05.76 SS.seek 25.00ml @08:26:06.37 Vacuum too high -- waiting up to 40s @08:26:06.63 Intake @51.6psia, vacuum from 20.0 to 24.0psi @08:26:06.64 SS.reconfigure forSampling @08:26:06.67 SS.seek 25.00ml @08:26:07.24 Vacuum too high -- waiting up to 40s @08:26:07.50 Intake @51.3psia, vacuum from 20.0 to 24.0psi @08:26:07.51 SS.reconfigure forSampling @08:26:07.54 SS.seek 25.00ml @08:26:09.46 Vacuum too high -- waiting up to 40s @08:26:09.72 Intake @50.9psia, vacuum from 20.0 to 24.0psi @08:26:09.73 SS.reconfigure forSampling @08:26:09.76 SS.seek 25.00ml @08:26:10.40 Vacuum too high -- waiting up to 40s @08:26:10.66 Intake @50.8psia, vacuum from 20.0 to 24.0psi @08:26:10.67 SS.reconfigure forSampling @08:26:10.70 SS.seek 25.00ml @08:26:11.46 Vacuum too high -- waiting up to 40s @08:26:11.73 SS.seek 25.00ml @08:26:12.37 Vacuum too high -- waiting up to 40s @08:26:12.63 Intake @50.8psia, vacuum from 20.0 to 24.0psi @08:26:12.64 SS.reconfigure forSampling @08:26:12.68 SS.seek 25.00ml @08:26:13.24 Vacuum too high -- waiting up to 40s @08:26:13.50 Intake @50.7psia, vacuum from 20.0 to 24.0psi @08:26:13.51 SS.reconfigure forSampling @08:26:13.54 SS.seek 25.00ml @08:26:14.15 Vacuum too high -- waiting up to 40s @08:26:14.41 Intake @50.6psia, vacuum from 20.0 to 24.0psi @08:26:14.42 SS.reconfigure forSampling @08:26:14.46 SS.seek 25.00ml @08:26:15.18 Vacuum too high -- waiting up to 40s @08:26:15.44 Intake @50.8psia, vacuum from 20.0 to 24.0psi @08:26:15.45 SS.reconfigure forSampling @08:26:15.48 SS.seek 25.00ml @08:26:16.24 Vacuum too high -- waiting up to 40s @08:26:16.71 Intake @51.7psia, vacuum from 20.0 to 24.0psi @08:26:16.72 SS.reconfigure forSampling @08:26:16.75 SS.seek 25.00ml @08:26:17.71 Vacuum too high -- waiting up to 40s @08:26:18.19 Intake @52.0psia, vacuum from 20.0 to 24.0psi @08:26:18.20 SS.reconfigure forSampling @08:26:18.23 SS.seek 25.00ml @08:26:19.24 Vacuum too high -- waiting up to 40s @08:26:19.50 Intake @51.5psia, vacuum from 20.0 to 24.0psi @08:26:19.51 SS.reconfigure forSampling @08:26:19.54 SS.seek 25.00ml @08:26:20.12 Vacuum too high -- waiting up to 40s @08:26:20.38 Intake @51.1psia, vacuum from 20.0 to 24.0psi @08:26:20.39 SS.reconfigure forSampling @08:26:20.42 SS.seek 25.00ml @08:26:21.12 Vacuum too high -- waiting up to 40s @08:26:21.38 Intake @50.9psia, vacuum from 20.0 to 24.0psi @08:26:21.39 SS.reconfigure forSampling @08:26:21.42 SS.seek 25.00ml @08:26:22.08 Vacuum too high -- waiting up to 40s @08:26:22.34 Intake @51.1psia, vacuum from 20.0 to 24.0psi @08:26:22.35 SS.reconfigure forSampling @08:26:22.38 SS.seek 25.00ml @08:26:22.96 Vacuum too high -- waiting up to 40s @08:26:23.22 Intake @50.8psia, vacuum from 20.0 to 24.0psi @08:26:23.23 SS.reconfigure forSampling @08:26:23.26 SS.seek 25.00ml @08:26:23.93 Vacuum too high -- waiting up to 40s @08:26:24.19 Intake @50.6psia, vacuum from 20.0 to 24.0psi @08:26:24.20 SS.reconfigure forSampling @08:26:24.23 SS.seek 25.00ml @08:26:24.87 Vacuum too high -- waiting up to 40s @08:26:25.34 Intake @50.3psia, vacuum from 20.0 to 24.0psi @08:26:25.35 SS.reconfigure forSampling @08:26:25.38 SS.seek 25.00ml @08:26:26.29 Vacuum too high -- waiting up to 40s @08:26:26.55 Intake @50.7psia, vacuum from 20.0 to 24.0psi @08:26:26.56 SS.reconfigure forSampling @08:26:26.60 SS.seek 25.00ml @08:26:27.18 Vacuum too high -- waiting up to 40s @08:26:27.43 Intake @50.9psia, vacuum from 20.0 to 24.0psi @08:26:27.44 SS.reconfigure forSampling @08:26:27.48 SS.seek 25.00ml @08:26:28.05 Vacuum too high -- waiting up to 40s @08:26:28.31 Intake @51.4psia, vacuum from 20.0 to 24.0psi @08:26:28.32 SS.reconfigure forSampling @08:26:28.35 SS.seek 25.00ml @08:26:28.96 Vacuum too high -- waiting up to 40s @08:26:29.22 Intake @51.5psia, vacuum from 20.0 to 24.0psi @08:26:29.23 SS.reconfigure forSampling @08:26:29.26 SS.seek 25.00ml @08:26:29.83 Vacuum too high -- waiting up to 40s @08:26:30.09 Intake @51.5psia, vacuum from 20.0 to 24.0psi @08:26:30.10 SS.reconfigure forSampling @08:26:30.14 SS.seek 25.00ml @08:26:30.74 Vacuum too high -- waiting up to 40s @08:26:31.00 Intake @51.1psia, vacuum from 20.0 to 24.0psi @08:26:31.01 SS.reconfigure forSampling @08:26:31.04 SS.seek 25.00ml @08:26:31.77 Vacuum too high -- waiting up to 40s @08:26:32.03 Intake @51.1psia, vacuum from 20.0 to 24.0psi @08:26:32.04 SS.reconfigure forSampling @08:26:32.07 SS.seek 25.00ml @08:26:32.68 Vacuum too high -- waiting up to 40s @08:26:33.58 Intake @50.9psia, vacuum from 20.0 to 24.0psi @08:26:33.59 SS.reconfigure forSampling @08:26:33.63 SS.seek 25.00ml @08:26:34.71 Vacuum too high -- waiting up to 40s @08:26:34.97 Intake @50.8psia, vacuum from 20.0 to 24.0psi @08:26:34.98 SS.reconfigure forSampling @08:26:35.01 SS.seek 25.00ml @08:26:35.61 Vacuum too high -- waiting up to 40s @08:26:35.87 Intake @50.8psia, vacuum from 20.0 to 24.0psi @08:26:35.88 SS.reconfigure forSampling @08:26:35.91 SS.seek 25.00ml @08:26:36.43 Vacuum too high -- waiting up to 40s @08:26:36.69 Intake @50.9psia, vacuum from 20.0 to 24.0psi @08:26:36.70 SS.reconfigure forSampling @08:26:36.73 SS.seek 25.00ml @08:26:37.27 Vacuum too high -- waiting up to 40s @08:26:37.54 SS.seek 25.00ml @08:26:38.15 Vacuum too high -- waiting up to 40s @08:26:38.64 Intake @50.9psia, vacuum from 20.0 to 24.0psi @08:26:38.65 SS.reconfigure forSampling @08:26:38.69 SS.seek 25.00ml @08:26:39.61 Vacuum too high -- waiting up to 40s @08:26:40.08 Intake @51.2psia, vacuum from 20.0 to 24.0psi @08:26:40.09 SS.reconfigure forSampling @08:26:40.12 SS.seek 25.00ml @08:26:40.90 Vacuum too high -- waiting up to 40s @08:26:41.16 Intake @51.4psia, vacuum from 20.0 to 24.0psi @08:26:41.17 SS.reconfigure forSampling @08:26:41.20 SS.seek 25.00ml @08:26:41.83 Vacuum too high -- waiting up to 40s @08:26:42.09 Intake @51.6psia, vacuum from 20.0 to 24.0psi @08:26:42.10 SS.reconfigure forSampling @08:26:42.13 SS.seek 25.00ml @08:26:42.77 Vacuum too high -- waiting up to 40s @08:26:43.03 Intake @51.5psia, vacuum from 20.0 to 24.0psi @08:26:43.04 SS.reconfigure forSampling @08:26:43.07 SS.seek 25.00ml @08:26:43.74 Vacuum too high -- waiting up to 40s @08:26:44.00 Intake @51.4psia, vacuum from 20.0 to 24.0psi @08:26:44.01 SS.reconfigure forSampling @08:26:44.04 SS.seek 25.00ml @08:26:44.65 Vacuum too high -- waiting up to 40s @08:26:44.91 Intake @51.1psia, vacuum from 20.0 to 24.0psi @08:26:44.92 SS.reconfigure forSampling @08:26:44.95 SS.seek 25.00ml @08:26:45.55 Vacuum too high -- waiting up to 40s @08:26:46.03 Intake @50.9psia, vacuum from 20.0 to 24.0psi @08:26:46.04 SS.reconfigure forSampling @08:26:46.08 SS.seek 25.00ml @08:26:47.05 Vacuum too high -- waiting up to 40s @08:26:47.31 Intake @50.7psia, vacuum from 20.0 to 24.0psi @08:26:47.32 SS.reconfigure forSampling @08:26:47.35 SS.seek 25.00ml @08:26:47.98 Vacuum too high -- waiting up to 40s @08:26:48.24 Intake @50.6psia, vacuum from 20.0 to 24.0psi @08:26:48.25 SS.reconfigure forSampling @08:26:48.29 SS.seek 25.00ml @08:26:48.84 Vacuum too high -- waiting up to 40s @08:26:49.10 Intake @50.4psia, vacuum from 20.0 to 24.0psi @08:26:49.11 SS.reconfigure forSampling @08:26:49.14 SS.seek 25.00ml @08:26:49.77 Vacuum too high -- waiting up to 40s @08:26:50.24 Intake @50.6psia, vacuum from 20.0 to 24.0psi @08:26:50.25 SS.reconfigure forSampling @08:26:50.28 SS.seek 25.00ml @08:26:51.11 Vacuum too high -- waiting up to 40s @08:26:51.59 Intake @50.8psia, vacuum from 20.0 to 24.0psi @08:26:51.60 SS.reconfigure forSampling @08:26:51.63 SS.seek 25.00ml @08:26:52.46 Vacuum too high -- waiting up to 40s @08:26:52.99 Intake @50.9psia, vacuum from 20.0 to 24.0psi @08:26:53.00 SS.reconfigure forSampling @08:26:53.07 SS.seek 25.00ml @08:26:53.96 Vacuum too high -- waiting up to 40s @08:26:54.22 Intake @51.1psia, vacuum from 20.0 to 24.0psi @08:26:54.23 SS.reconfigure forSampling @08:26:54.26 SS.seek 25.00ml @08:26:54.77 Vacuum too high -- waiting up to 40s @08:26:55.03 Intake @51.4psia, vacuum from 20.0 to 24.0psi @08:26:55.04 SS.reconfigure forSampling @08:26:55.07 SS.seek 25.00ml @08:26:55.58 Vacuum too high -- waiting up to 40s @08:26:55.84 Intake @51.5psia, vacuum from 20.0 to 24.0psi @08:26:55.85 SS.reconfigure forSampling @08:26:55.88 SS.seek 25.00ml @08:26:56.52 Vacuum too high -- waiting up to 40s @08:26:56.79 SS.seek 25.00ml @08:26:57.27 Vacuum too high -- waiting up to 40s @08:26:58.15 Intake @51.2psia, vacuum from 20.0 to 24.0psi @08:26:58.16 SS.reconfigure forSampling @08:26:58.20 SS.seek 25.00ml @08:26:59.27 Vacuum too high -- waiting up to 40s @08:26:59.53 Intake @51.2psia, vacuum from 20.0 to 24.0psi @08:26:59.54 SS.reconfigure forSampling @08:26:59.57 SS.seek 25.00ml @08:27:00.14 Vacuum too high -- waiting up to 40s @08:27:00.63 Intake @51.0psia, vacuum from 20.0 to 24.0psi @08:27:00.64 SS.reconfigure forSampling @08:27:00.67 SS.seek 25.00ml @08:27:01.49 Vacuum too high -- waiting up to 40s @08:27:01.96 Intake @50.9psia, vacuum from 20.0 to 24.0psi @08:27:01.97 SS.reconfigure forSampling @08:27:02.00 SS.seek 25.00ml @08:27:02.68 Vacuum too high -- waiting up to 40s @08:27:03.15 Intake @50.8psia, vacuum from 20.0 to 24.0psi @08:27:03.16 SS.reconfigure forSampling @08:27:03.19 SS.seek 25.00ml @08:27:03.93 Vacuum too high -- waiting up to 40s @08:27:04.19 Intake @50.7psia, vacuum from 20.0 to 24.0psi @08:27:04.20 SS.reconfigure forSampling @08:27:04.23 SS.seek 25.00ml @08:27:04.80 Vacuum too high -- waiting up to 40s @08:27:05.06 Intake @50.8psia, vacuum from 20.0 to 24.0psi @08:27:05.07 SS.reconfigure forSampling @08:27:05.10 SS.seek 25.00ml @08:27:05.68 Vacuum too high -- waiting up to 40s @08:27:06.15 Intake @50.9psia, vacuum from 20.0 to 24.0psi @08:27:06.16 SS.reconfigure forSampling @08:27:06.19 SS.seek 25.00ml @08:27:06.86 Vacuum too high -- waiting up to 40s @08:27:07.33 Intake @51.2psia, vacuum from 20.0 to 24.0psi @08:27:07.34 SS.reconfigure forSampling @08:27:07.38 SS.seek 25.00ml @08:27:08.18 Vacuum too high -- waiting up to 40s @08:27:08.65 Intake @51.5psia, vacuum from 20.0 to 24.0psi @08:27:08.66 SS.reconfigure forSampling @08:27:08.69 SS.seek 25.00ml @08:27:09.58 Vacuum too high -- waiting up to 40s @08:27:10.05 Intake @51.5psia, vacuum from 20.0 to 24.0psi @08:27:10.06 SS.reconfigure forSampling @08:27:10.09 SS.seek 25.00ml @08:27:11.05 Vacuum too high -- waiting up to 40s @08:27:11.30 Intake @51.3psia, vacuum from 20.0 to 24.0psi @08:27:11.31 SS.reconfigure forSampling @08:27:11.35 SS.seek 25.00ml @08:27:11.99 Vacuum too high -- waiting up to 40s @08:27:12.25 Intake @51.1psia, vacuum from 20.0 to 24.0psi @08:27:12.26 SS.reconfigure forSampling @08:27:12.29 SS.seek 25.00ml @08:27:12.86 Vacuum too high -- waiting up to 40s @08:27:13.12 Intake @50.8psia, vacuum from 20.0 to 24.0psi @08:27:13.13 SS.reconfigure forSampling @08:27:13.16 SS.seek 25.00ml @08:27:13.71 Vacuum too high -- waiting up to 40s @08:27:13.97 Intake @50.8psia, vacuum from 20.0 to 24.0psi @08:27:13.98 SS.reconfigure forSampling @08:27:14.02 SS.seek 25.00ml @08:27:14.64 Vacuum too high -- waiting up to 40s @08:27:15.12 Intake @50.8psia, vacuum from 20.0 to 24.0psi @08:27:15.13 SS.reconfigure forSampling @08:27:15.16 SS.seek 25.00ml @08:27:15.86 Vacuum too high -- waiting up to 40s @08:27:16.12 Intake @50.6psia, vacuum from 20.0 to 24.0psi @08:27:16.13 SS.reconfigure forSampling @08:27:16.16 SS.seek 25.00ml @08:27:16.71 Vacuum too high -- waiting up to 40s @08:27:17.17 Intake @50.7psia, vacuum from 20.0 to 24.0psi @08:27:17.18 SS.reconfigure forSampling @08:27:17.22 SS.seek 25.00ml @08:27:17.96 Vacuum too high -- waiting up to 40s @08:27:18.22 Intake @50.6psia, vacuum from 20.0 to 24.0psi @08:27:18.23 SS.reconfigure forSampling @08:27:18.26 SS.seek 25.00ml @08:27:18.83 Vacuum too high -- waiting up to 40s @08:27:19.30 Intake @51.4psia, vacuum from 20.0 to 24.0psi @08:27:19.31 SS.reconfigure forSampling @08:27:19.35 SS.seek 25.00ml @08:27:19.99 Vacuum too high -- waiting up to 40s @08:27:20.47 Intake @51.9psia, vacuum from 20.0 to 24.0psi @08:27:20.48 SS.reconfigure forSampling @08:27:20.51 SS.seek 25.00ml @08:27:21.21 Vacuum too high -- waiting up to 40s @08:27:21.46 Intake @51.6psia, vacuum from 20.0 to 24.0psi @08:27:21.47 SS.reconfigure forSampling @08:27:22.15 SS.seek 25.00ml @08:27:23.05 Vacuum too high -- waiting up to 40s @08:27:23.31 Intake @50.5psia, vacuum from 20.0 to 24.0psi @08:27:23.32 SS.reconfigure forSampling @08:27:23.35 SS.seek 25.00ml @08:27:23.93 Vacuum too high -- waiting up to 40s @08:27:24.40 Intake @50.3psia, vacuum from 20.0 to 24.0psi @08:27:24.41 SS.reconfigure forSampling @08:27:24.45 SS.seek 25.00ml @08:27:25.30 Vacuum too high -- waiting up to 40s @08:27:25.77 Intake @50.3psia, vacuum from 20.0 to 24.0psi @08:27:25.78 SS.reconfigure forSampling @08:27:25.81 SS.seek 25.00ml @08:27:26.67 Vacuum too high -- waiting up to 40s @08:27:27.14 Intake @50.1psia, vacuum from 20.0 to 24.0psi @08:27:27.15 SS.reconfigure forSampling @08:27:27.18 SS.seek 25.00ml @08:27:27.92 Vacuum too high -- waiting up to 40s @08:27:28.40 Intake @49.8psia, vacuum from 20.0 to 24.0psi @08:27:28.41 SS.reconfigure forSampling @08:27:28.44 SS.seek 25.00ml @08:27:29.21 Vacuum too high -- waiting up to 40s @08:27:29.68 SS.seek 25.00ml @08:27:30.33 Vacuum too high -- waiting up to 40s @08:27:30.80 Intake @50.2psia, vacuum from 20.0 to 24.0psi @08:27:30.81 SS.reconfigure forSampling @08:27:30.84 SS.seek 25.00ml @08:27:31.58 Vacuum too high -- waiting up to 40s @08:27:32.06 Intake @51.5psia, vacuum from 20.0 to 24.0psi @08:27:32.07 SS.reconfigure forSampling @08:27:32.10 SS.seek 25.00ml @08:27:32.80 Vacuum too high -- waiting up to 40s @08:27:33.28 Intake @51.4psia, vacuum from 20.0 to 24.0psi @08:27:33.29 SS.reconfigure forSampling @08:27:33.32 SS.seek 25.00ml @08:27:34.11 Vacuum too high -- waiting up to 40s @08:27:34.37 Intake @51.1psia, vacuum from 20.0 to 24.0psi @08:27:34.38 SS.reconfigure forSampling @08:27:34.41 SS.seek 25.00ml @08:27:34.89 Vacuum too high -- waiting up to 40s @08:27:35.14 Intake @49.9psia, vacuum from 20.0 to 24.0psi @08:27:35.16 SS.reconfigure forSampling @08:27:35.19 SS.seek 25.00ml @08:27:35.74 Vacuum too high -- waiting up to 40s @08:27:36.22 Intake @50.1psia, vacuum from 20.0 to 24.0psi @08:27:36.23 SS.reconfigure forSampling @08:27:36.26 SS.seek 25.00ml @08:27:36.99 Vacuum too high -- waiting up to 40s @08:27:37.47 Intake @50.3psia, vacuum from 20.0 to 24.0psi @08:27:37.48 SS.reconfigure forSampling @08:27:37.51 SS.seek 25.00ml @08:27:38.17 Vacuum too high -- waiting up to 40s @08:27:38.64 Intake @50.5psia, vacuum from 20.0 to 24.0psi @08:27:38.65 SS.reconfigure forSampling @08:27:38.68 SS.seek 25.00ml @08:27:39.39 Vacuum too high -- waiting up to 40s @08:27:39.85 Intake @50.8psia, vacuum from 20.0 to 24.0psi @08:27:39.86 SS.reconfigure forSampling @08:27:39.90 SS.seek 25.00ml @08:27:40.52 Vacuum too high -- waiting up to 40s @08:27:40.98 Intake @51.4psia, vacuum from 20.0 to 24.0psi @08:27:40.99 SS.reconfigure forSampling @08:27:41.03 SS.seek 25.00ml @08:27:41.71 Vacuum too high -- waiting up to 40s @08:27:42.17 Intake @51.2psia, vacuum from 20.0 to 24.0psi @08:27:42.18 SS.reconfigure forSampling @08:27:42.22 SS.seek 25.00ml @08:27:43.05 Vacuum too high -- waiting up to 40s @08:27:43.31 Intake @50.8psia, vacuum from 20.0 to 24.0psi @08:27:43.32 SS.reconfigure forSampling @08:27:43.36 SS.seek 25.00ml @08:27:43.89 Vacuum too high -- waiting up to 40s @08:27:44.15 Intake @50.6psia, vacuum from 20.0 to 24.0psi @08:27:44.16 SS.reconfigure forSampling @08:27:44.20 SS.seek 25.00ml @08:27:44.74 Vacuum too high -- waiting up to 40s @08:27:45.21 Intake @50.4psia, vacuum from 20.0 to 24.0psi @08:27:45.22 SS.reconfigure forSampling @08:27:45.26 SS.seek 25.00ml @08:27:45.99 Vacuum too high -- waiting up to 40s @08:27:47.11 Intake @50.5psia, vacuum from 20.0 to 24.0psi @08:27:47.12 SS.reconfigure forSampling @08:27:47.15 SS.seek 25.00ml @08:27:48.33 Vacuum too high -- waiting up to 40s @08:27:48.81 SS.seek 25.00ml @08:27:49.45 Vacuum too high -- waiting up to 40s @08:27:49.93 Intake @51.1psia, vacuum from 20.0 to 24.0psi @08:27:49.94 SS.reconfigure forSampling @08:27:49.98 SS.seek 25.00ml @08:27:50.74 Vacuum too high -- waiting up to 40s @08:27:51.21 Intake @51.2psia, vacuum from 20.0 to 24.0psi @08:27:51.22 SS.reconfigure forSampling @08:27:51.25 SS.seek 25.00ml @08:27:51.89 Vacuum too high -- waiting up to 40s @08:27:52.16 SS.seek 25.00ml @08:27:52.61 Vacuum too high -- waiting up to 40s @08:27:52.87 Intake @50.8psia, vacuum from 20.0 to 24.0psi @08:27:52.88 SS.reconfigure forSampling @08:27:52.91 SS.seek 25.00ml @08:27:53.45 Vacuum too high -- waiting up to 40s @08:27:53.71 Intake @50.7psia, vacuum from 20.0 to 24.0psi @08:27:53.72 SS.reconfigure forSampling @08:27:53.76 SS.seek 25.00ml @08:27:54.27 Vacuum too high -- waiting up to 40s @08:27:54.74 Intake @50.5psia, vacuum from 20.0 to 24.0psi @08:27:54.75 SS.reconfigure forSampling @08:27:54.78 SS.seek 25.00ml @08:27:55.49 Vacuum too high -- waiting up to 40s @08:27:55.75 Intake @50.5psia, vacuum from 20.0 to 24.0psi @08:27:55.76 SS.reconfigure forSampling @08:27:55.79 SS.seek 25.00ml @08:27:56.24 Vacuum too high -- waiting up to 40s @08:27:56.71 Intake @50.6psia, vacuum from 20.0 to 24.0psi @08:27:56.72 SS.reconfigure forSampling @08:27:56.75 SS.seek 25.00ml @08:27:57.49 Vacuum too high -- waiting up to 40s @08:27:57.96 Intake @50.8psia, vacuum from 20.0 to 24.0psi @08:27:57.97 SS.reconfigure forSampling @08:27:58.00 SS.seek 25.00ml @08:27:58.80 Vacuum too high -- waiting up to 40s @08:27:59.27 Intake @51.0psia, vacuum from 20.0 to 24.0psi @08:27:59.28 SS.reconfigure forSampling @08:27:59.31 SS.seek 25.00ml @08:27:59.89 Vacuum too high -- waiting up to 40s @08:28:00.36 Intake @51.1psia, vacuum from 20.0 to 24.0psi @08:28:00.37 SS.reconfigure forSampling @08:28:00.40 SS.seek 25.00ml @08:28:01.10 Vacuum too high -- waiting up to 40s @08:28:01.36 Intake @51.0psia, vacuum from 20.0 to 24.0psi @08:28:01.37 SS.reconfigure forSampling @08:28:01.40 SS.seek 25.00ml @08:28:01.92 Vacuum too high -- waiting up to 40s @08:28:02.18 Intake @50.6psia, vacuum from 20.0 to 24.0psi @08:28:02.19 SS.reconfigure forSampling @08:28:02.22 SS.seek 25.00ml @08:28:02.80 Vacuum too high -- waiting up to 40s @08:28:03.27 Intake @50.4psia, vacuum from 20.0 to 24.0psi @08:28:03.28 SS.reconfigure forSampling @08:28:03.32 SS.seek 25.00ml @08:28:04.08 Vacuum too high -- waiting up to 40s @08:28:04.55 Intake @50.7psia, vacuum from 20.0 to 24.0psi @08:28:04.56 SS.reconfigure forSampling @08:28:04.60 SS.seek 25.00ml @08:28:05.24 Vacuum too high -- waiting up to 40s @08:28:05.70 Intake @50.9psia, vacuum from 20.0 to 24.0psi @08:28:05.71 SS.reconfigure forSampling @08:28:05.75 SS.seek 25.00ml @08:28:06.33 Vacuum too high -- waiting up to 40s @08:28:06.80 Intake @50.9psia, vacuum from 20.0 to 24.0psi @08:28:06.81 SS.reconfigure forSampling @08:28:06.84 SS.seek 25.00ml @08:28:07.42 Vacuum too high -- waiting up to 40s @08:28:07.91 Intake @50.8psia, vacuum from 20.0 to 24.0psi @08:28:07.92 SS.reconfigure forSampling @08:28:07.95 SS.seek 25.00ml @08:28:08.74 Vacuum too high -- waiting up to 40s @08:28:09.20 Intake @51.3psia, vacuum from 20.0 to 24.0psi @08:28:09.21 SS.reconfigure forSampling @08:28:09.25 SS.seek 25.00ml @08:28:09.99 Vacuum too high -- waiting up to 40s @08:28:10.92 Intake @51.5psia, vacuum from 20.0 to 24.0psi @08:28:10.93 SS.reconfigure forSampling @08:28:10.96 SS.seek 25.00ml @08:28:12.14 Vacuum too high -- waiting up to 40s @08:28:12.61 Intake @50.8psia, vacuum from 20.0 to 24.0psi @08:28:12.62 SS.reconfigure forSampling @08:28:12.66 SS.seek 25.00ml @08:28:13.42 Vacuum too high -- waiting up to 40s @08:28:13.90 Intake @50.8psia, vacuum from 20.0 to 24.0psi @08:28:13.91 SS.reconfigure forSampling @08:28:13.95 SS.seek 25.00ml @08:28:14.64 Vacuum too high -- waiting up to 40s @08:28:15.11 Intake @51.1psia, vacuum from 20.0 to 24.0psi @08:28:15.12 SS.reconfigure forSampling @08:28:15.15 SS.seek 25.00ml @08:28:15.77 Vacuum too high -- waiting up to 40s @08:28:16.24 Intake @51.3psia, vacuum from 20.0 to 24.0psi @08:28:16.25 SS.reconfigure forSampling @08:28:16.29 SS.seek 25.00ml @08:28:16.92 Vacuum too high -- waiting up to 40s @08:28:17.40 Intake @51.0psia, vacuum from 20.0 to 24.0psi @08:28:17.41 SS.reconfigure forSampling @08:28:17.44 SS.seek 25.00ml @08:28:18.14 Vacuum too high -- waiting up to 40s @08:28:18.62 Intake @51.1psia, vacuum from 20.0 to 24.0psi @08:28:18.63 SS.reconfigure forSampling @08:28:18.66 SS.seek 25.00ml @08:28:19.30 Vacuum too high -- waiting up to 40s @08:28:19.77 Intake @50.8psia, vacuum from 20.0 to 24.0psi @08:28:19.78 SS.reconfigure forSampling @08:28:19.81 SS.seek 25.00ml @08:28:20.42 Vacuum too high -- waiting up to 40s @08:28:20.89 Intake @50.5psia, vacuum from 20.0 to 24.0psi @08:28:20.90 SS.reconfigure forSampling @08:28:20.93 SS.seek 25.00ml @08:28:21.52 Vacuum too high -- waiting up to 40s @08:28:21.78 Intake @50.2psia, vacuum from 20.0 to 24.0psi @08:28:21.79 SS.reconfigure forSampling @08:28:21.82 SS.seek 25.00ml @08:28:22.33 Vacuum too high -- waiting up to 40s @08:28:22.80 Intake @50.0psia, vacuum from 20.0 to 24.0psi @08:28:22.81 SS.reconfigure forSampling @08:28:22.84 SS.seek 25.00ml @08:28:23.58 Vacuum too high -- waiting up to 40s @08:28:24.05 Intake @50.3psia, vacuum from 20.0 to 24.0psi @08:28:24.06 SS.reconfigure forSampling @08:28:24.09 SS.seek 25.00ml @08:28:24.67 Vacuum too high -- waiting up to 40s @08:28:25.15 Intake @51.5psia, vacuum from 20.0 to 24.0psi @08:28:25.16 SS.reconfigure forSampling @08:28:25.20 SS.seek 25.00ml @08:28:25.80 Vacuum too high -- waiting up to 40s @08:28:26.26 Intake @52.1psia, vacuum from 20.0 to 24.0psi @08:28:26.27 SS.reconfigure forSampling @08:28:26.31 SS.seek 25.00ml @08:28:26.86 Vacuum too high -- waiting up to 40s @08:28:27.34 Intake @50.9psia, vacuum from 20.0 to 24.0psi @08:28:27.35 SS.reconfigure forSampling @08:28:27.38 SS.seek 25.00ml @08:28:28.17 Vacuum too high -- waiting up to 40s @08:28:28.63 Intake @50.4psia, vacuum from 20.0 to 24.0psi @08:28:28.64 SS.reconfigure forSampling @08:28:28.68 SS.seek 25.00ml @08:28:29.52 Vacuum too high -- waiting up to 40s @08:28:29.98 Intake @50.3psia, vacuum from 20.0 to 24.0psi @08:28:29.99 SS.reconfigure forSampling @08:28:30.03 SS.seek 25.00ml @08:28:30.70 Vacuum too high -- waiting up to 40s @08:28:31.17 Intake @50.2psia, vacuum from 20.0 to 24.0psi @08:28:31.18 SS.reconfigure forSampling @08:28:31.21 SS.seek 25.00ml @08:28:31.92 Vacuum too high -- waiting up to 40s @08:28:32.39 SS.seek 25.00ml @08:28:33.02 Vacuum too high -- waiting up to 40s @08:28:33.48 Intake @50.2psia, vacuum from 20.0 to 24.0psi @08:28:33.49 SS.reconfigure forSampling @08:28:33.53 SS.seek 25.00ml @08:28:34.89 Vacuum too high -- waiting up to 40s @08:28:35.16 Intake @50.6psia, vacuum from 20.0 to 24.0psi @08:28:35.17 SS.reconfigure forSampling @08:28:35.21 SS.seek 25.00ml @08:28:36.42 Vacuum too high -- waiting up to 40s @08:28:36.89 Intake @51.3psia, vacuum from 20.0 to 24.0psi @08:28:36.90 SS.reconfigure forSampling @08:28:36.93 SS.seek 25.00ml @08:28:37.58 Vacuum too high -- waiting up to 40s @08:28:38.05 Intake @51.8psia, vacuum from 20.0 to 24.0psi @08:28:38.07 SS.reconfigure forSampling @08:28:38.10 SS.seek 25.00ml @08:28:38.83 Vacuum too high -- waiting up to 40s @08:28:39.31 Intake @51.2psia, vacuum from 20.0 to 24.0psi @08:28:39.32 SS.reconfigure forSampling @08:28:39.35 SS.seek 25.00ml @08:28:40.14 Vacuum too high -- waiting up to 40s @08:28:40.62 Intake @50.7psia, vacuum from 20.0 to 24.0psi @08:28:40.63 SS.reconfigure forSampling @08:28:40.66 SS.seek 25.00ml @08:28:41.26 Vacuum too high -- waiting up to 40s @08:28:41.73 Intake @50.3psia, vacuum from 20.0 to 24.0psi @08:28:41.74 SS.reconfigure forSampling @08:28:41.77 SS.seek 25.00ml @08:28:42.45 Vacuum too high -- waiting up to 40s @08:28:42.92 Intake @50.6psia, vacuum from 20.0 to 24.0psi @08:28:42.93 SS.reconfigure forSampling @08:28:42.96 SS.seek 25.00ml @08:28:43.51 Vacuum too high -- waiting up to 40s @08:28:43.99 Intake @50.5psia, vacuum from 20.0 to 24.0psi @08:28:44.00 SS.reconfigure forSampling @08:28:44.04 SS.seek 25.00ml @08:28:44.73 Vacuum too high -- waiting up to 40s @08:28:45.20 Intake @50.4psia, vacuum from 20.0 to 24.0psi @08:28:45.21 SS.reconfigure forSampling @08:28:45.25 SS.seek 25.00ml @08:28:45.95 Vacuum too high -- waiting up to 40s @08:28:46.43 Intake @50.5psia, vacuum from 20.0 to 24.0psi @08:28:46.44 SS.reconfigure forSampling @08:28:46.47 SS.seek 25.00ml @08:28:47.17 Vacuum too high -- waiting up to 40s @08:28:47.64 Intake @51.2psia, vacuum from 20.0 to 24.0psi @08:28:47.65 SS.reconfigure forSampling @08:28:47.69 SS.seek 25.00ml @08:28:48.30 Vacuum too high -- waiting up to 40s @08:28:48.78 Intake @51.7psia, vacuum from 20.0 to 24.0psi @08:28:48.79 SS.reconfigure forSampling @08:28:48.83 SS.seek 25.00ml @08:28:49.48 Vacuum too high -- waiting up to 40s @08:28:49.95 Intake @51.2psia, vacuum from 20.0 to 24.0psi @08:28:49.96 SS.reconfigure forSampling @08:28:49.99 SS.seek 25.00ml @08:28:50.64 Vacuum too high -- waiting up to 40s @08:28:51.12 Intake @50.6psia, vacuum from 20.0 to 24.0psi @08:28:51.13 SS.reconfigure forSampling @08:28:51.16 SS.seek 25.00ml @08:28:51.83 Vacuum too high -- waiting up to 40s @08:28:51.90 SS.reconfigure SSconfig @08:28:51.94 SS.hold @08:28:52.03 Intake.select :close @08:28:52.17 ShallowSampler::Clogged in MAIN -- progress too slow after 194.5ml SSV.dial :exhaust @08:28:52.98 Exhaust.select :open @08:28:53.08 SS.reconfigure SSexhale @08:28:53.12 SS.seek 0.00ml @08:29:00.71 Exhaust.select :close @08:29:00.81 Sampled 194.5ml @08:29:00.82 collectSample: Complete Evacuating Puck (bubblePt=55.0psi, delay=15.0s) @08:29:00.83 Intake.select :close @08:29:00.89 CSV.dial :waste @08:29:00.92 CS.seek 0.000ml @08:29:00.99 SSV.dial CBV,avoiding => exhaust @08:29:01.02
CBV.dial SSV @08:29:01.05 CSV.dial :air @08:29:01.89 CS.seek 10.000ml @08:29:03.00
CTV.dial :air @08:29:03.78 delay 15 seconds @08:29:03.80 Sleepy.power :sampler,:OFF @08:29:18.78
SSV.dial :spit,avoiding => exhaust @08:29:20.11 CTV.dial CSV,avoiding => intake @08:29:20.13
SS.seek 0.00ml @08:29:20.21 SSV.dial CBV,avoiding => exhaust @08:29:26.62 CBV.dial between CSV and waste2b @08:29:27.55 CSV.dial CTV @08:29:28.16 CTV.dial CSV,avoiding => air @08:29:28.61
CS.reconfigure CSpressurize @08:29:28.64 CS.seek 0.000ml @08:29:52.58 BubblePoint: Intake @70.2psia, Outlet @14.6psia @08:29:52.59 SS.reconfigure SSconfig @08:29:52.62 SS.seek 10.00ml @08:29:58.10 delay 15 seconds @08:30:13.20 Evacuated: Intake @69.5psia, Outlet @-0.2psia @08:30:13.21 SSV.dial :spit,avoiding => exhaust @08:30:14.53 SS.reconfigure SSexhale @08:30:14.56 SS.seek 0.00ml @08:30:19.47 SS.reconfigure SSfast @08:30:19.51 SS.seek 5.00ml @08:30:22.58 SSV.dial CBV,avoiding => exhaust @08:30:22.60
CBV.dial SSV @08:30:23.95 CTV.dial :air,avoiding => intake @08:30:25.25 CSV.dial CTV @08:30:25.27
SS.reconfigure SSconfig @08:30:25.31 SS.seek 10.00ml @08:30:26.27 CSV.dial :air @08:30:26.79 CS.reconfigure CSfast @08:30:26.82 CS.seek 0.000ml @08:30:28.72
SSV.dial :spit,avoiding => exhaust @08:30:29.41 CS.coast @08:30:30.05
SS.reconfigure SSexhale @08:30:30.08 SS.seek 0.00ml @08:30:34.96 SS.reconfigure SSfast @08:30:34.99 SS.seek 5.00ml @08:30:37.89 SSV.dial CBV,avoiding => exhaust @08:30:37.91
CBV.dial SSV @08:30:40.22 SSV.dial :spit,avoiding => exhaust @08:30:41.54 SS.reconfigure SSconfig @08:30:41.57 SS.seek 0.00ml @08:30:44.61 SS.coast @08:30:44.64 Evacuating Puck (bubblePt=55.0psi, delay=15.0s) Intake.select :close @08:30:44.70 CSV.dial :waste @08:30:44.79 SSV.dial CBV,avoiding => exhaust @08:30:44.81
CBV.dial SSV @08:30:45.45 CS.seek 0.000ml @08:30:45.50 CSV.dial :air @08:30:46.14
CTV.dial :air @08:30:46.15 delay 15 seconds @08:30:46.31 CS.seek 10.000ml @08:31:01.15
SSV.dial :spit,avoiding => exhaust @08:31:02.48 CTV.dial CSV,avoiding => intake @08:31:02.50
SS.reconfigure SSexhale @08:31:02.57 SS.seek 0.00ml @08:31:02.61 SSV.dial CBV,avoiding => exhaust @08:31:10.42 CBV.dial between CSV and waste2b @08:31:11.35 CSV.dial between air and CTV @08:31:11.65 CS.seek 7.255ml @08:31:18.82 CTV.dial CSV,avoiding => air @08:31:18.83
CSV.dial CTV @08:31:19.34 CS.reconfigure CSpressurize @08:31:19.37 CS.seek 0.000ml @08:31:38.28 CSV.dial :air @08:31:38.71 CS.reconfigure CSfast @08:31:38.74 CS.seek 10.000ml @08:32:02.54 CSV.dial between air and CTV @08:32:02.93 CS.seek 2.126ml @08:32:21.69 CTV.dial CSV,avoiding => air @08:32:21.70
CSV.dial CTV @08:32:21.99 CS.reconfigure CSpressurize @08:32:22.02 CS.seek 0.000ml @08:32:23.66 BubblePoint: Intake @73.8psia, Outlet @18.4psia @08:32:23.67 SS.reconfigure SSconfig @08:32:23.71 SS.seek 10.00ml @08:32:29.28 delay 15 seconds @08:32:44.37 Evacuated: Intake @72.9psia, Outlet @-0.0psia @08:32:44.38 SSV.dial :spit,avoiding => exhaust @08:32:45.73 SS.reconfigure SSexhale @08:32:45.76 SS.seek 0.00ml @08:32:50.90 SS.reconfigure SSfast @08:32:50.93 SS.seek 5.00ml @08:32:53.54 SSV.dial CBV,avoiding => exhaust @08:32:53.55
CBV.dial SSV @08:32:54.91 CTV.dial :air,avoiding => intake @08:32:56.20 CSV.dial CTV @08:32:56.22
SS.reconfigure SSconfig @08:32:56.26 SS.seek 10.00ml @08:32:57.22 CSV.dial :air @08:32:57.74 CS.reconfigure CSfast @08:32:57.77 CS.seek 0.000ml @08:32:59.45
SSV.dial :spit,avoiding => exhaust @08:33:00.77 SS.reconfigure SSexhale @08:33:00.80 SS.seek 0.00ml @08:33:02.53 CS.coast @08:33:05.71
SS.reconfigure SSfast @08:33:05.74 SS.seek 5.00ml @08:33:08.72 SSV.dial CBV,avoiding => exhaust @08:33:08.73
CBV.dial SSV @08:33:11.06 SSV.dial :spit,avoiding => exhaust @08:33:12.38 SS.reconfigure SSconfig @08:33:12.98 SS.seek 0.00ml @08:33:16.01 SS.coast @08:33:16.04 Lysing: Adding 1.5ml lysis CBV.dial between CSV and waste1,avoiding => DBV @08:33:17.52 CTV.dial CSV @08:33:18.78 CSV.dial CSR @08:33:19.57 CSR.select :lysis @08:33:19.67 CS.reconfigure CSconfig @08:33:19.71 CS.seek 1.000ml @08:33:23.03 delay 5 seconds @08:33:28.04 CSR.select :air @08:33:28.18 CS.seek 6.000ml @08:33:41.65 delay 2 seconds @08:33:43.66 CSV.dial :waste1 @08:33:45.14 CS.reconfigure CSfast @08:33:45.17 CS.seek empty @08:33:59.81 CSV.dial CSR @08:34:01.07 CSR.select :lysis @08:34:01.17 CS.reconfigure CSconfig @08:34:01.21 CS.seek 1.500ml @08:34:05.80 delay 5 seconds @08:34:10.81 CSR.select :air @08:34:10.95 CS.seek 4.500ml @08:34:19.34 delay 2 seconds @08:34:21.35 CSV.dial CTV @08:34:21.78 CS.seek 1.275ml @08:34:30.81 Heating sh1 puck from tube 3 in Collection Clamp to 85.0C for 5:00 @08:34:30.82 CH.seek 85.0C,8:00 @08:37:11.71 CH.hold 5:00,85.0C @08:42:11.80 Collection Puck Heater at 84.9C after lysing @08:42:11.81 CS.seek 0.000ml @08:42:15.80 CTV.dial between spare and air,avoiding => intake @08:42:16.79 CSV.dial :air @08:42:17.24 CS.seek 5.000ml @08:42:30.67 CSV.dial CTV @08:42:31.18 delay 2 seconds @08:42:33.19 CSV.dial :air @08:42:33.70 delay 2 seconds @08:42:35.71 CSV.dial CTV @08:42:36.23 delay 2 seconds @08:42:38.24 CSV.dial :air @08:42:38.76 delay 2 seconds @08:42:40.77 CS.seek 0.000ml @08:42:54.32 Recovering Lysate into Collection syringe @08:42:54.33 CSV.dial CBV @08:42:55.31 CBV.dial CSV @08:42:55.65 CS.seek 5.000ml @08:43:09.17 delay 5 seconds @08:43:14.18 CTV.dial :air @08:43:14.67 CS.seek 6.999ml @08:43:20.54 delay 5 seconds @08:43:25.55 CSV.dial :air2 @08:43:25.99 CS.seek 10.000ml @08:43:34.38 CSV.dial between air and CTV,avoiding => CBV @08:43:35.72 Lysing Complete @08:43:35.76 Storing sh1 puck in Collection Clamp to tube 2 @08:43:35.89 SC.reconfigure SCtight @08:43:35.92 SC.seek 2 @08:43:38.11 SC.reconfigure SCloose @08:43:38.27 Elbow.reconfigure TightElbow @08:43:38.31 Elbow.seek 2 @08:43:39.97 Forearm.reconfigure TightForearm @08:43:40.00 Forearm.seek 2 + 800 counts @08:43:41.01 SE.reconfigure SEpick @08:43:41.06 SE.seek beamBreak @08:43:44.04 SE.configure SEconfig @08:43:44.16 Elbow.reconfigure LimpElbow @08:43:44.19 Forearm.reconfigure LooseForearm @08:43:44.23 Forearm.seek 2 @08:43:44.44 Elbow.hold @08:43:44.49 Elbow.reconfigure TightElbow @08:43:44.57 SE.seek 1.04 Pucks below Top Plate @08:43:44.87 Forearm.coast @08:43:44.90 Forearm.seek 2 + 316 counts @08:43:45.13 Forearm.reconfigure TightForearm @08:43:45.16 Forearm.seek 2 + 800 counts @08:43:45.45 SE.seek 2.04 Pucks below Top Plate @08:43:45.99 Forearm.reconfigure LooseForearm @08:43:46.03 Forearm.seek retract @08:43:46.95 Elbow.seek CC @08:43:48.53 Elbow.reconfigure LooseElbow @08:43:48.62 Forearm.seek CC @08:43:49.56 CC.seek sealed @08:43:50.51 Hand.grip @08:43:51.22 CC.seek open @08:43:54.54 Forearm.seek retract @08:43:55.47 Elbow.reconfigure TightElbow @08:43:55.51 Elbow.seek 2 @08:43:57.11 Elbow.reconfigure LimpElbow @08:43:57.14 Forearm.seek 2 @08:43:58.10 Elbow.hold @08:43:58.15 Elbow.reconfigure TightElbow @08:43:58.19 Hand.release @08:43:59.05 Forearm.coast @08:43:59.09 Forearm.seek 2 + 333 counts @08:43:59.31 Forearm.reconfigure TightForearm @08:43:59.95 Forearm.seek 2 + 800 counts @08:44:00.27 SE.seek down @08:44:01.38
Forearm.reconfigure LooseForearm @08:44:01.42 Forearm.seek retract @08:44:02.78 SE.coast @08:44:02.81
SC.coast @08:44:02.83 Tranferring lysate to PRV @08:44:02.84 CSV.dial :air @08:44:03.22 CS.seek 10.000ml @08:44:03.29 CSV.dial between air and CTV @08:44:03.67 AIV.dial :bypass @08:44:03.83 Beginning samplePassC @08:44:03.84 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:44:03.99 Elbow.seek FlushPuck::Garage @08:44:05.76 Elbow.reconfigure LooseElbow @08:44:05.84 Forearm.seek garage @08:44:06.83 Hand.grip @08:44:07.68 Forearm.seek retract @08:44:08.69 Elbow.reconfigure TightElbow @08:44:08.73 Elbow.seek CC @08:44:11.47 Elbow.reconfigure LooseElbow @08:44:11.56 Forearm.reconfigure TightForearm @08:44:11.59 Forearm.seek CC @08:44:12.69 CC.seek closed @08:44:16.53 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 2.04 @08:44:16.59 Hand.release @08:44:17.38 Forearm.reconfigure LooseForearm @08:44:17.41 Forearm.seek retract @08:44:18.35 AWV.select :air @08:44:18.36 AIV.dial :bypass @08:44:18.37 PSV.dial PSR @08:44:19.77 PSR.select :flush @08:44:19.87 PS.seek 1.500ml @08:44:35.96 delay 5 seconds @08:44:40.97 PSV.dial :waste1 @08:44:41.44 PS.seek 0.000ml @08:44:57.49 PSV.dial PSR @08:44:57.94 PSR.select :air @08:44:58.04 PS.seek 2.500ml @08:45:24.37 CBV.dial PRV @08:45:24.38 PRV.dial CBV @08:45:25.13
PSV.dial :mixloop @08:45:26.24 CTV.dial :air,avoiding => intake @08:45:26.25 PTV.dial :PRVmixing @08:45:26.33 PS.seek 0.000ml @08:45:52.58 PSV.dial :air @08:45:53.04 PS.seek 2.500ml @08:46:19.24 PSV.dial :mixloop @08:46:19.68 PS.seek 0.000ml @08:46:45.99 PSV.dial :air @08:46:46.56 PS.seek 2.500ml @08:47:12.77 PSV.dial :mixloop @08:47:13.20 PS.seek 0.000ml @08:47:39.42 Garaging FlushPuck in Collection Clamp @08:47:39.48 Elbow.reconfigure TightElbow @08:47:39.51 Elbow.seek CC @08:47:39.60 Elbow.reconfigure LooseElbow @08:47:39.69 Forearm.seek CC @08:47:40.66 CC.seek sealed @08:47:41.56 Hand.grip @08:47:42.33 CC.seek open @08:47:45.62 Forearm.seek retract @08:47:46.56 Elbow.reconfigure TightElbow @08:47:46.60 Elbow.seek garage @08:47:49.41 Elbow.reconfigure LooseElbow @08:47:49.50 Forearm.reconfigure TightForearm @08:47:49.53 Forearm.seek garage @08:47:50.67 Hand.release @08:47:51.50 Forearm.reconfigure LooseForearm @08:47:51.53 Forearm.seek retract @08:47:52.54 Connecting PS to CS to transfer sample @08:47:52.55 CBV.dial AIV @08:47:53.20 AIV.dial :bypass @08:47:53.21 PSV.dial :mixloop PTV.dial :PRVmixing @08:47:53.22 PRV.dial AIV @08:47:53.23 CTV.dial :air,avoiding => intake @08:47:53.24 Loading lyfil puck from tube 3 into Collection Clamp @08:47:53.42 SC.reconfigure SCtight @08:47:53.45 SC.seek 3 @08:47:55.69 SC.reconfigure SCloose @08:47:55.86 Elbow.reconfigure TightElbow @08:47:55.89 Elbow.seek 3 @08:47:57.71 Forearm.reconfigure TightForearm @08:47:57.74 Forearm.seek 3 + 800 counts @08:47:58.83 SE.reconfigure SEpick @08:47:58.88 SE.seek beamBreak @08:48:00.07 SE.configure SEconfig @08:48:00.19 Elbow.seek 3 @08:48:00.34 Forearm.seek 3 + 300 counts @08:48:00.71 Forearm.reconfigure LooseForearm @08:48:00.75 Hand.grip @08:48:01.47 SE.seek down @08:48:01.61
Forearm.seek retract @08:48:02.49 SE.coast @08:48:02.55
Elbow.seek CC @08:48:04.11 Elbow.reconfigure LooseElbow @08:48:04.19 Forearm.reconfigure TightForearm @08:48:04.22 Forearm.seek CC @08:48:05.34 CC.seek closed @08:48:09.15 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.96 @08:48:09.16 Hand.release @08:48:09.87 Forearm.reconfigure LooseForearm @08:48:09.90 Forearm.seek retract @08:48:10.84 SC.coast @08:48:10.86 CTV.dial CSV,avoiding => intake @08:48:12.17 CSV.dial CTV @08:48:12.46 CS.seek 5.000ml @08:48:25.96 delay 1:20 @08:49:45.97 CS.seek 2.500ml @08:49:53.15 delay 40 seconds @08:50:33.16 CS.seek 0.000ml @08:50:40.27 delay 1:00 @08:51:40.29 PSV.dial :air @08:51:40.73 PS.seek 2.500ml @08:52:07.01 PSV.dial :mixloop @08:52:07.45 delay 3 seconds @08:52:10.46 PSV.dial :air @08:52:10.89 delay 2 seconds @08:52:12.90 PSV.dial :mixloop @08:52:13.34 delay 3 seconds @08:52:16.35 PSV.dial :waste1 @08:52:17.63 PS.seek empty @08:52:43.86 CBV.dial :waste1 @08:52:44.32 delay 5 seconds @08:52:49.33 CBV.dial AIV @08:52:49.78 delay 10 seconds @08:52:59.79 CBV.dial :waste1 @08:53:00.24 delay 10 seconds @08:53:10.25 CBV.dial AIV @08:53:10.70 delay 10 seconds @08:53:20.71 CTV.dial CSV,avoiding => intake @08:53:20.72 CBV.dial between AIV and waste1 @08:53:21.06 CSV.dial :air @08:53:21.60 CS.seek 10.000ml @08:53:47.74 CSV.dial CTV @08:53:48.27 CS.seek 0.000ml @08:54:14.40 CBV.dial :waste1 @08:54:14.69 delay 10 seconds @08:54:24.70 CTV.dial :air,avoiding => intake @08:54:26.01 Storing lyfil puck in Collection Clamp to tube 2 @08:54:26.14 SC.reconfigure SCtight @08:54:26.17 SC.seek 2 @08:54:28.37 SC.reconfigure SCloose @08:54:28.53 Elbow.reconfigure TightElbow @08:54:28.57 Elbow.seek 2 @08:54:30.19 Forearm.reconfigure TightForearm @08:54:30.22 Forearm.seek 2 + 800 counts @08:54:31.29 SE.reconfigure SEpick @08:54:31.34 SE.seek beamBreak @08:54:34.08 SE.configure SEconfig @08:54:34.19 Elbow.reconfigure LimpElbow @08:54:34.23 Forearm.reconfigure LooseForearm @08:54:34.27 Forearm.seek 2 @08:54:34.47 Elbow.hold @08:54:34.52 Elbow.reconfigure TightElbow @08:54:35.20 SE.seek 2.04 Pucks below Top Plate @08:54:35.53 Forearm.coast @08:54:35.56 Forearm.seek 2 + 341 counts @08:54:35.84 Forearm.reconfigure TightForearm @08:54:35.87 Forearm.seek 2 + 800 counts @08:54:36.04 SE.seek 3.04 Pucks below Top Plate @08:54:36.58 Forearm.reconfigure LooseForearm @08:54:36.61 Forearm.seek retract @08:54:37.53 Elbow.seek CC @08:54:39.04 Elbow.reconfigure LooseElbow @08:54:39.13 Forearm.seek CC @08:54:40.09 CC.seek sealed @08:54:41.02 Hand.grip @08:54:41.70 CC.seek open @08:54:45.03 Forearm.seek retract @08:54:45.99 Elbow.reconfigure TightElbow @08:54:46.03 Elbow.seek 2 @08:54:47.61 Elbow.reconfigure LimpElbow @08:54:47.64 Forearm.seek 2 @08:54:48.60 Elbow.hold @08:54:48.65 Elbow.reconfigure TightElbow @08:54:48.68 Hand.release @08:54:49.53 Forearm.coast @08:54:49.56 Forearm.seek 2 + 337 counts @08:54:49.78 Forearm.reconfigure TightForearm @08:54:49.81 Forearm.seek 2 + 800 counts @08:54:50.13 SE.seek down @08:54:51.23
Forearm.reconfigure LooseForearm @08:54:51.27 Forearm.seek retract @08:54:52.54 SE.coast @08:54:52.57
SC.coast @08:54:52.59 PSV.dial :air @08:54:53.65 PS.seek 0.499ml @08:54:59.63 delay 3 seconds @08:55:02.64 PSV.dial :mixloop @08:55:03.07 PRV.dial :air @08:55:03.52 delay 3 seconds @08:55:06.53 PRV.dial CBV @08:55:06.98 CBV.dial AIV @08:55:07.44 PRV.dial between CBV and air @08:55:07.76 PRV.dial :air @08:55:08.09 PS.reconfigure PSslow @08:55:08.13 PS.seek 0.599ml @08:55:10.73 PRV.dial :waste2 @08:55:11.46 PS.reconfigure PSconfig @08:55:11.49 PS.seek empty @08:55:18.51 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:55:18.65 Elbow.seek FlushPuck::Garage @08:55:20.40 Elbow.reconfigure LooseElbow @08:55:20.49 Forearm.seek garage @08:55:21.52 Hand.grip @08:55:22.28 Forearm.seek retract @08:55:23.28 Elbow.reconfigure TightElbow @08:55:23.32 Elbow.seek CC @08:55:26.07 Elbow.reconfigure LooseElbow @08:55:26.16 Forearm.reconfigure TightForearm @08:55:26.19 Forearm.seek CC @08:55:27.25 CC.seek closed @08:55:30.99 Collection Clamp onSpring/offSpring for 5/22 samples, on/off torque 2.03 @08:55:31.04 Hand.release @08:55:31.86 Forearm.reconfigure LooseForearm @08:55:31.89 Forearm.seek retract @08:55:32.88 Clearing PML for modified lysate CTV.dial :air,avoiding => intake @08:55:32.89 PRV.dial :waste2 @08:55:32.90 PTV.dial :PRV,avoiding => puck @08:55:33.60 delay 2 seconds @08:55:36.23 PTV.dial :PRVmixing,avoiding => puck @08:55:36.94 delay 2 seconds @08:55:38.95 PSV.dial :PML @08:55:38.96 delay 2 seconds @08:55:40.96 PRV.dial :air @08:55:41.69 delay 2 seconds @08:55:43.75 PS.seek 1.499ml @08:55:59.91 delay 5 seconds @08:56:04.93 PS.seek 2.500ml @08:56:15.91 PSV.dial :air @08:56:16.36 PS.seek 0.000ml @08:56:42.57 mixing lysate with speDil @08:56:42.58 PSV.dial :PML @08:56:43.02 CBV.dial :PRV @08:56:43.03 PRV.dial :air @08:56:43.04 delay 2 seconds @08:56:45.05 PRV.dial :speDil @08:56:45.49 delay 2 seconds @08:56:47.55 PS.reconfigure PSslow1 @08:56:47.58 PS.seek 0.225ml @08:57:10.88 delay 5 seconds @08:57:15.90 PRV.dial CBV @08:57:16.61 delay 2 seconds @08:57:18.67 PS.seek 0.475ml @08:57:44.35 delay 5 seconds @08:57:49.36 PRV.dial :air @08:57:49.81 delay 2 seconds @08:57:51.82 PS.seek 0.925ml @08:58:38.06 delay 10 seconds @08:58:48.08 PRV.dial CBV @08:58:48.53 CTV.dial :air,avoiding => intake @08:58:48.54 PSV.dial :air @08:58:48.99 PS.reconfigure PSconfig @08:58:49.02 PS.seek 2.500ml @08:59:05.87 delay 3 seconds @08:59:08.89 PSV.dial PTV @08:59:09.61 PTV.dial :PRV,avoiding => puck @08:59:10.35 PS.seek 0.000ml @08:59:36.75 PSV.dial :air @08:59:37.48 PS.seek 2.500ml @09:00:03.72 delay 3 seconds @09:00:06.74 PSV.dial PTV @09:00:07.47 PTV.dial :PRV,avoiding => puck @09:00:07.49 PS.seek 0.000ml @09:00:33.68 Pushing modified lysate into AIV Injection Loop @09:00:33.69 CBV.dial AIV @09:00:33.70 AIV.dial :bypass @09:00:33.71 PRV.dial CBV @09:00:33.72 PSV.dial :air @09:00:34.44 PS.seek 2.500ml @09:01:00.68 delay 3 seconds @09:01:03.69 PSV.dial :PML @09:01:04.12 PTV.dial :PRVmixing,avoiding => puck @09:01:04.89 PS.reconfigure PSslow1 @09:01:04.92 PS.seek 1.850ml @09:02:11.55 delay 5 seconds @09:02:16.56 AIV.dial :sample @09:02:17.10 AWV.select :waste @09:02:17.26 PS.reconfigure PSslow2 @09:02:17.29 PS.seek 1.310ml @09:04:07.58 delay 15 seconds @09:04:22.59 AIV.dial :bypass @09:04:23.15 PS.reconfigure PSconfig @09:04:23.18 PS.seek 0.000ml @09:04:37.33 PSV.dial :air @09:04:37.78 PS.seek 2.500ml @09:05:04.02 PSV.dial :PML @09:05:04.46 PS.seek 0.000ml @09:05:30.77 AIV.dial :coilToMFB @09:05:30.78 AWV.select :air @09:05:30.92 Extracting Nucleic Acid from AIV @09:05:30.94
Beginning samplePassD @09:05:30.95 SPE.hold 2:46:40,55.0C @09:05:30.97 ASV.dial :SPEdelivery @09:05:31.09
AWV.select :air @09:05:31.10 AIV.dial :bypass @09:05:31.11 PSV.dial PSR @09:05:31.81 delay 20 seconds @09:05:32.14
PSR.select :killMFB @09:05:32.24 PS.seek 1.500ml @09:05:48.30 delay 5 seconds @09:05:51.82 primeLysateINT @09:05:51.83 AWV.select :air @09:05:51.84 AIV.dial :coilToMFB ATV.dial :AIV @09:05:53.26 AS.reconfigure ASslow1 @09:05:53.29 AS.seek 0.239ml @09:05:53.32
PSV.dial :waste1 @09:05:53.78 PS.seek 0.000ml @09:06:09.89 PSV.dial PSR @09:06:10.34 PSR.select :air @09:06:10.44 PS.seek 2.500ml @09:06:36.73 PRV.dial CBV @09:06:36.75
CBV.dial PRV @09:06:36.76 PSV.dial :mixloop @09:06:37.80 CTV.dial :air,avoiding => intake @09:06:37.81 PTV.dial :PRVmixing @09:06:37.83 PS.seek 0.000ml @09:06:42.51 delay 10 seconds @09:06:52.52 ATV.dial :air2 @09:06:53.10 AS.seek 0.269ml @09:06:59.44 ATV.dial :waste2 @09:06:59.80 AS.reconfigure ASnorm @09:06:59.83 AS.seek empty @09:07:04.04
PSV.dial PSR @09:07:05.09 PSR.select :flush @09:07:05.75 PS.seek 1.500ml @09:07:13.99 Lysate primed! @09:07:14.00 AFV.select :holdingCoil @09:07:14.01 ATV.dial :air2 @09:07:14.40 AS.reconfigure ASslow1 @09:07:14.43 AS.seek 0.069ml @09:07:21.79
delay 5 seconds @09:07:26.80 PSV.dial :waste1 @09:07:27.24 PS.seek 0.000ml @09:07:28.99 delay 3 seconds @09:07:32.00 ATV.dial :AIV @09:07:32.57 AS.seek 0.469ml @09:07:43.32
PSV.dial PSR @09:07:43.78 PSR.select :air @09:07:43.88 PS.seek 2.500ml @09:08:10.17 PRV.dial CBV @09:08:10.19
CBV.dial PRV @09:08:10.20 PSV.dial :mixloop @09:08:11.34 CTV.dial :air,avoiding => intake @09:08:11.35 PTV.dial :PRVmixing @09:08:11.37 PS.seek 0.000ml @09:08:37.63 PSV.dial PSR @09:08:38.65 PSR.select :flush @09:08:38.75 PS.seek 1.500ml @09:08:54.36 delay 5 seconds @09:08:54.83
delay 5 seconds @09:08:59.41 Solid Phase Extraction Heater at 51.8C @09:08:59.42 ATV.dial ASV @09:08:59.75 ASV.dial :SPEdelivery @09:08:59.81 AS.seek 0.041ml @09:08:59.84
PSV.dial :waste1 @09:09:00.29 PS.seek 0.000ml @09:09:16.33 PSV.dial PSR @09:09:16.78 PSR.select :air @09:09:16.88 PS.seek 2.500ml @09:09:43.17 PRV.dial CBV @09:09:43.19
CBV.dial PRV @09:09:43.20 PSV.dial :mixloop @09:09:44.31 CTV.dial :air,avoiding => intake PTV.dial :PRVmixing @09:09:44.33 PS.seek 0.000ml @09:10:10.64 PSV.dial PSR @09:10:11.66 PSR.select :flush @09:10:11.76 PS.seek 1.500ml @09:10:27.35 delay 10 seconds @09:10:27.86
delay 5 seconds @09:10:32.88 PSV.dial :waste1 @09:10:33.32 PS.seek 0.000ml @09:10:37.36 rinseLysateLineINT @09:10:37.37 AFV.select :carrierSolution @09:10:37.46 delay 2 seconds @09:10:39.47 AS.reconfigure ASconfig @09:10:39.50 AS.seek 1.000ml @09:10:49.36
PSV.dial PSR @09:10:49.92 PSR.select :air @09:10:50.02 PS.seek 2.500ml @09:11:16.23 PRV.dial CBV @09:11:16.25
CBV.dial PRV @09:11:16.26 PSV.dial :mixloop @09:11:17.28 CTV.dial :air,avoiding => intake @09:11:17.29 PTV.dial :PRVmixing @09:11:17.30 PS.reconfigure PSslow @09:11:17.34 PS.seek 1.200ml @09:11:35.68 delay 1 second @09:11:36.69 AFV.select :holdingCoil @09:11:36.78 delay 1 second @09:11:37.79 ATV.dial :AIV @09:11:38.21 AIV.dial :coilToMFB @09:11:38.22 AWV.select :waste @09:11:38.31 AS.reconfigure ASslow0 @09:11:38.35 AS.seek 0.000ml @09:11:44.29
PSV.dial :waste1 @09:11:45.60 PS.reconfigure PSconfig @09:11:45.63 PS.seek 0.000ml @09:11:58.67 PSV.dial :air @09:11:59.69 PS.seek 2.500ml @09:12:25.95 PSV.dial :mixloop @09:12:26.40 PS.reconfigure PSslow @09:12:26.43 PS.seek 0.000ml @09:13:17.92 PSV.dial :air @09:13:18.36 PS.reconfigure PSconfig @09:13:18.39 PS.seek 2.500ml @09:13:20.65 ATV.dial :air2 @09:13:21.29 AS.reconfigure ASslow1 @09:13:21.33 AS.seek 0.050ml @09:13:31.85 ATV.dial :AIV @09:13:32.43 AS.seek 0.010ml @09:13:40.83 ATV.dial :waste @09:13:42.12 AS.seek empty @09:13:44.54 ethanol wash @09:13:44.55 ATV.dial :air2 @09:13:44.66
PSV.dial :mixloop @09:13:45.11 PS.reconfigure PSslow @09:13:45.14 PS.seek 0.000ml @09:13:45.49 AS.reconfigure ASslow2 @09:13:45.52 AS.seek 0.009ml @09:13:56.22 delay 3 seconds @09:13:59.23 Pulling 0.4ml of Ethanol Wash @09:13:59.30 ATV.dial :ethanol @09:13:59.86 AS.reconfigure ASslow1 @09:13:59.90 AS.seek 0.409ml @09:14:36.41
Cleaning Collection Syringe @09:14:36.42 CSV.dial CSR @09:14:36.84 CSR.select :flush @09:14:36.94 CS.seek 4.000ml @09:14:47.92 delay 5 seconds @09:14:52.94 CSR.select :air @09:14:53.03 CS.seek 10.000ml @09:15:09.01 CSV.dial CTV @09:15:09.03
CTV.dial CSV @09:15:10.93 CBV.dial :waste1 @09:15:11.41 CS.seek 0.000ml @09:15:21.80 delay 3 seconds @09:15:24.81 ATV.dial :air1 @09:15:25.14 delay 3 seconds @09:15:28.20 AS.seek 0.434ml @09:15:33.53 delay 3 seconds @09:15:36.54 ATV.dial :ethanol @09:15:36.91 AS.seek 0.419ml @09:15:37.54
CSV.dial CSR @09:15:38.04 CSR.select :flush @09:15:38.14 CS.seek 4.000ml @09:15:40.20 delay 3 seconds @09:15:43.21 ATV.dial ASV @09:15:44.44 AS.seek 0.092ml @09:15:49.19
delay 5 seconds @09:15:54.20 CSR.select :air @09:15:54.29 CS.seek 10.000ml @09:16:10.35 CSV.dial CTV @09:16:10.37
CTV.dial CSV @09:16:10.89 CBV.dial :waste1 @09:16:10.90 CS.seek 0.000ml @09:16:37.15 Garaging FlushPuck in Collection Clamp @09:16:37.20 Elbow.reconfigure TightElbow @09:16:37.23 Elbow.seek CC @09:16:37.33 Elbow.reconfigure LooseElbow @09:16:37.42 Forearm.seek CC @09:16:38.39 CC.seek sealed @09:16:39.32 Hand.grip @09:16:40.03 CC.seek open @09:16:43.38 Forearm.seek retract @09:16:44.32 Elbow.reconfigure TightElbow @09:16:44.36 Elbow.seek garage @09:16:47.17 Elbow.reconfigure LooseElbow @09:16:47.26 Forearm.reconfigure TightForearm @09:16:47.29 Forearm.seek garage @09:16:48.44 Hand.release @09:16:49.19 Forearm.reconfigure LooseForearm @09:16:49.22 Forearm.seek retract @09:16:50.24 Whole Cell Archive sampling 1000ml @09:16:50.26 Loading wcr puck from tube 3 into Collection Clamp @09:16:50.43 SC.reconfigure SCtight @09:16:50.46 SC.seek 3 @09:16:51.37 delay 3 seconds @09:16:52.70 SC.reconfigure SCloose @09:16:52.87 Elbow.reconfigure TightElbow @09:16:52.91 Elbow.seek 3 @09:16:54.38 ATV.dial :waste2 @09:16:54.78 Forearm.reconfigure TightForearm @09:16:54.81 Forearm.seek 3 + 800 counts @09:16:54.99 AS.reconfigure ASconfig @09:16:55.02 AS.seek empty @09:16:55.91 SE.reconfigure SEpick @09:16:55.96 SE.seek beamBreak @09:16:57.22 SE.configure SEconfig @09:16:57.34 Elbow.seek 3 @09:16:57.48 Forearm.seek 3 + 300 counts @09:16:57.77 Forearm.reconfigure LooseForearm @09:16:57.81 Hand.grip @09:16:58.54 SE.seek down @09:16:58.68 Forearm.seek retract @09:16:59.64 SE.coast @09:16:59.69 Elbow.seek CC @09:17:00.75 ATV.dial :air2 @09:17:01.10 AS.reconfigure ASslow1 @09:17:01.15 AS.seek 1.000ml @09:17:01.25 Elbow.reconfigure LooseElbow @09:17:01.33 Forearm.reconfigure TightForearm @09:17:01.36 Forearm.seek CC @09:17:02.51 CC.seek closed @09:17:06.59 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.96 @09:17:06.60 Hand.release @09:17:07.32 Forearm.reconfigure LooseForearm @09:17:07.36 Forearm.seek retract @09:17:08.33 SC.coast @09:17:08.36 collectSample 1000ml w/bubble point of 55psi @09:17:08.38 Priming 7ml Sleepy.power :sampler,:ON @09:17:08.39 Intake.select :close @09:17:08.40 Exhaust.select :close @09:17:08.41 SS.hold @09:17:08.56 Calibrating pressure sensors @09:17:08.57 CTV.dial :air @09:17:09.89 SSV.dial :spit,avoiding => exhaust @09:17:09.90 SS.reconfigure SSfast @09:17:09.93 SS.seek 5.00ml @09:17:12.84 SSV.dial CBV @09:17:12.85 CBV.dial SSV @09:17:14.28 delay 3.8 seconds @09:17:18.17 SSV.dial :spit @09:17:19.53 SS.seek 0.00ml @09:17:22.05 SSV.dial CBV @09:17:23.39 CTV.dial :intake @09:17:24.13 Waiting for external sampler to finish priming... @09:17:24.14 delayUntil "09:27:08.38" @09:20:25.60 delay 5 seconds @09:20:30.61 ATV.dial ASV @09:20:31.32 ASV.dial :SPEdelivery @09:20:31.34 AS.seek 0.020ml @09:23:51.50 delay 5 seconds @09:23:56.51 ATV.dial :air2 @09:23:57.27 AS.seek 0.520ml @09:25:39.51 delay 5 seconds @09:25:44.53 ATV.dial ASV @09:25:45.24 ASV.dial :wasteR @09:25:45.72 AS.seek 0.300ml @09:26:30.85 delay 20 seconds @09:26:50.86 AS.seek 0.070ml @09:27:08.39 SSV.dial CBV @09:27:08.41 CBV.dial SSV @09:27:08.42 Allowing 55s for 7.0ml of remaining 7.0ml @09:27:08.43 Intake.select :open @09:27:10.56 Intake @52.9psia, vacuum from 20.0 to 24.0psi @09:27:10.58 SS.reconfigure forSampling @09:27:10.61 SS.seek 7.00ml @09:27:16.26 Stroke completed -- waiting for pressure to equalize ... @09:27:16.90 Intake.select :close @09:27:17.03 SSV.dial :exhaust @09:27:17.81 Exhaust.select :open @09:27:17.92 SS.reconfigure SSexhale @09:27:17.96 SS.seek 0.00ml @09:27:21.89 Exhaust.select :close @09:27:22.00 Sampling 1000.0ml @09:27:22.01 Intake.select :close @09:27:22.02 Exhaust.select :close @09:27:22.03 SS.reconfigure SSconfig @09:27:22.06 SS.hold @09:27:22.21 CTV.dial :intake @09:27:22.22 SSV.dial CBV @09:27:22.24 CBV.dial SSV @09:27:23.02 Allowing 185s for 25.0ml of remaining 1000.0ml @09:27:23.03 Intake.select :open @09:27:25.16 Intake @53.5psia, vacuum from 20.0 to 24.0psi @09:27:25.18 SS.reconfigure forSampling @09:27:25.21 SS.seek 25.00ml @09:27:37.99 delay 20 seconds @09:27:40.06 Stroke completed -- waiting for pressure to equalize ... @09:27:40.70 Intake.select :close @09:27:40.83 SSV.dial :exhaust @09:27:41.60 Exhaust.select :open @09:27:41.71 SS.reconfigure SSexhale @09:27:41.75 SS.seek 0.00ml @09:27:50.42 Exhaust.select :close @09:27:50.53 SSV.dial CBV @09:27:50.55 CBV.dial SSV @09:27:51.34 Allowing 185s for 25.0ml of remaining 975.0ml @09:27:51.35 Intake.select :open @09:27:53.49 Intake @52.5psia, vacuum from 20.0 to 24.0psi @09:27:53.51 SS.reconfigure forSampling @09:27:53.54 SS.seek 25.00ml @09:27:58.00 ATV.dial :waste2 @09:27:58.55 AS.reconfigure ASconfig @09:27:58.58 AS.seek empty @09:27:59.06 Vacuum too high -- waiting up to 40s @09:27:59.33 Intake @44.9psia, vacuum from 20.0 to 24.0psi @09:27:59.35 SS.reconfigure forSampling @09:27:59.38 SS.seek 25.00ml @09:28:03.00 Elution @09:28:03.01 ATV.dial :air1 @09:28:04.04 AS.reconfigure ASslow1 @09:28:04.08 AS.seek 0.049ml @09:28:09.77 Stroke completed -- waiting for pressure to equalize ... @09:28:10.41 Intake.select :close @09:28:10.55 SSV.dial :exhaust @09:28:11.34 Exhaust.select :open @09:28:11.44 SS.reconfigure SSexhale @09:28:11.48 SS.seek 0.00ml @09:28:14.58 delay 3 seconds @09:28:17.59 ATV.dial :ElutionWater @09:28:18.16 AS.seek 0.109ml @09:28:20.26 Exhaust.select :close @09:28:20.36 SSV.dial CBV @09:28:20.38 CBV.dial SSV @09:28:21.78 Allowing 185s for 25.0ml of remaining 950.0ml @09:28:21.79 Intake.select :open @09:28:23.92 Intake @52.3psia, vacuum from 20.0 to 24.0psi @09:28:23.94 SS.reconfigure forSampling @09:28:23.97 SS.seek 25.00ml @09:28:27.22 Vacuum too high -- waiting up to 40s @09:28:27.48 Intake @46.9psia, vacuum from 20.0 to 24.0psi @09:28:27.50 SS.reconfigure forSampling @09:28:27.53 SS.seek 25.00ml @09:28:30.06 Vacuum too high -- waiting up to 40s @09:28:30.54 Intake @48.7psia, vacuum from 20.0 to 24.0psi @09:28:30.55 SS.reconfigure forSampling @09:28:30.59 SS.seek 25.00ml @09:28:30.64 delay 3 seconds @09:28:33.19 Vacuum too high -- waiting up to 40s @09:28:33.46 Intake @47.4psia, vacuum from 20.0 to 24.0psi @09:28:33.48 SS.reconfigure forSampling @09:28:33.51 SS.seek 25.00ml @09:28:33.65 ATV.dial ASV,via => air2 @09:28:35.43 ASV.dial :SPEelution @09:28:35.78 Vacuum too high -- waiting up to 40s @09:28:36.27 Intake @50.3psia, vacuum from 20.0 to 24.0psi @09:28:36.29 SS.reconfigure forSampling @09:28:36.32 SS.seek 25.00ml @09:28:36.37 AFV.select :carrierSolution @09:28:36.55 AS.reconfigure ASnorm @09:28:36.58 AS.seek 0.759ml @09:28:39.09 Vacuum too high -- waiting up to 40s @09:28:39.36 Intake @48.8psia, vacuum from 20.0 to 24.0psi @09:28:39.37 SS.reconfigure forSampling @09:28:39.41 SS.seek 25.00ml @09:28:47.61 Vacuum too high -- waiting up to 40s @09:28:48.09 Intake @50.6psia, vacuum from 20.0 to 24.0psi @09:28:48.10 SS.reconfigure forSampling @09:28:48.14 SS.seek 25.00ml @09:28:58.63 Stroke completed -- waiting for pressure to equalize ... @09:28:59.27 Intake.select :close @09:28:59.41 SSV.dial :exhaust @09:29:00.19 Exhaust.select :open @09:29:00.30 SS.reconfigure SSexhale @09:29:00.34 SS.seek 0.00ml @09:29:09.18 Exhaust.select :close @09:29:09.29 SSV.dial CBV @09:29:09.31 CBV.dial SSV @09:29:10.08 delay 3 seconds @09:29:10.13 Allowing 185s for 25.0ml of remaining 925.0ml @09:29:10.14 Intake.select :open @09:29:12.28 Intake @51.3psia, vacuum from 20.0 to 24.0psi @09:29:12.30 SS.reconfigure forSampling @09:29:12.33 SS.seek 25.00ml @09:29:13.09 AFV.select :holdingCoil @09:29:13.23 AS.reconfigure ASslow1 @09:29:13.26 AS.seek 0.489ml @09:29:14.93 Vacuum too high -- waiting up to 40s @09:29:15.20 Intake @49.7psia, vacuum from 20.0 to 24.0psi @09:29:15.21 SS.reconfigure forSampling @09:29:15.25 SS.seek 25.00ml @09:29:48.52 Vacuum too high -- waiting up to 40s @09:29:48.79 Intake @50.5psia, vacuum from 20.0 to 24.0psi @09:29:48.80 SS.reconfigure forSampling @09:29:48.84 SS.seek 25.00ml @09:30:08.56 delay 5 seconds @09:30:13.62 AS.reconfigure ASslow2 @09:30:13.65 AS.seek 0.454ml @09:30:31.36 Stroke completed -- waiting for pressure to equalize ... @09:30:32.59 Intake.select :close @09:30:32.72 SSV.dial :exhaust @09:30:33.50 Exhaust.select :open @09:30:33.61 SS.reconfigure SSexhale @09:30:33.65 SS.seek 0.00ml @09:30:42.94 Exhaust.select :close @09:30:43.05 SSV.dial CBV @09:30:43.07 CBV.dial SSV @09:30:43.88 Allowing 185s for 25.0ml of remaining 900.0ml @09:30:43.89 Intake.select :open @09:30:46.04 Intake @50.6psia, vacuum from 20.0 to 24.0psi @09:30:46.06 SS.reconfigure forSampling @09:30:46.09 SS.seek 25.00ml @09:30:49.69 delay 1:00 @09:31:49.70 pushing eluate to holding coil @09:31:49.75 AS.seek 0.294ml @09:32:11.54 Vacuum too high -- waiting up to 40s @09:32:11.81 Intake @48.8psia, vacuum from 20.0 to 24.0psi @09:32:11.82 SS.reconfigure forSampling @09:32:11.85 SS.seek 25.00ml @09:32:26.13 Vacuum too high -- waiting up to 40s @09:32:26.61 Intake @48.3psia, vacuum from 20.0 to 24.0psi @09:32:26.62 SS.reconfigure forSampling @09:32:26.65 SS.seek 25.00ml @09:32:34.45 Vacuum too high -- waiting up to 40s @09:32:34.93 Intake @48.7psia, vacuum from 20.0 to 24.0psi @09:32:35.55 SS.reconfigure forSampling @09:32:35.59 SS.seek 25.00ml @09:32:38.96 Stroke completed -- waiting for pressure to equalize ... @09:32:41.34 Intake.select :close @09:32:41.47 SSV.dial :exhaust @09:32:42.28 Exhaust.select :open @09:32:42.38 SS.reconfigure SSexhale @09:32:42.42 SS.seek 0.00ml @09:32:51.12 Exhaust.select :close @09:32:51.23 SSV.dial CBV @09:32:51.25 CBV.dial SSV @09:32:52.07 Allowing 185s for 25.0ml of remaining 875.0ml @09:32:52.08 Intake.select :open @09:32:54.22 Intake @49.3psia, vacuum from 20.0 to 24.0psi @09:32:54.24 SS.reconfigure forSampling @09:32:54.27 SS.seek 25.00ml @09:32:58.98 Vacuum too high -- waiting up to 40s @09:32:59.46 Intake @48.9psia, vacuum from 20.0 to 24.0psi @09:32:59.47 SS.reconfigure forSampling @09:32:59.51 SS.seek 25.00ml @09:33:03.69 Vacuum too high -- waiting up to 40s @09:33:04.19 SS.seek 25.00ml @09:33:07.35 Vacuum too high -- waiting up to 40s @09:33:07.84 Intake @48.8psia, vacuum from 20.0 to 24.0psi @09:33:07.85 SS.reconfigure forSampling @09:33:07.88 SS.seek 25.00ml @09:33:11.47 Vacuum too high -- waiting up to 40s @09:33:11.96 Intake @49.3psia, vacuum from 20.0 to 24.0psi @09:33:11.97 SS.reconfigure forSampling @09:33:12.01 SS.seek 25.00ml @09:33:14.33 Vacuum too high -- waiting up to 40s @09:33:14.60 Intake @48.6psia, vacuum from 20.0 to 24.0psi @09:33:14.62 SS.reconfigure forSampling @09:33:14.65 SS.seek 25.00ml @09:33:16.98 Vacuum too high -- waiting up to 40s @09:33:17.46 Intake @48.9psia, vacuum from 20.0 to 24.0psi @09:33:17.47 SS.reconfigure forSampling @09:33:17.50 SS.seek 25.00ml @09:33:20.30 Vacuum too high -- waiting up to 40s @09:33:20.77 Intake @49.6psia, vacuum from 20.0 to 24.0psi @09:33:20.79 SS.reconfigure forSampling @09:33:20.82 SS.seek 25.00ml @09:33:22.92 Vacuum too high -- waiting up to 40s @09:33:23.40 Intake @49.0psia, vacuum from 20.0 to 24.0psi @09:33:23.41 SS.reconfigure forSampling @09:33:23.44 SS.seek 25.00ml @09:33:25.51 Vacuum too high -- waiting up to 40s @09:33:26.00 Intake @48.0psia, vacuum from 20.0 to 24.0psi @09:33:26.01 SS.reconfigure forSampling @09:33:26.05 SS.seek 25.00ml @09:33:28.51 Vacuum too high -- waiting up to 40s @09:33:28.99 Intake @48.6psia, vacuum from 20.0 to 24.0psi @09:33:29.01 SS.reconfigure forSampling @09:33:29.04 SS.seek 25.00ml @09:33:31.14 Vacuum too high -- waiting up to 40s @09:33:31.61 Intake @48.4psia, vacuum from 20.0 to 24.0psi @09:33:31.62 SS.reconfigure forSampling @09:33:31.65 SS.seek 25.00ml @09:33:33.76 Vacuum too high -- waiting up to 40s @09:33:34.23 Intake @48.3psia, vacuum from 20.0 to 24.0psi @09:33:34.24 SS.reconfigure forSampling @09:33:34.28 SS.seek 25.00ml @09:33:36.08 Vacuum too high -- waiting up to 40s @09:33:36.55 Intake @48.1psia, vacuum from 20.0 to 24.0psi @09:33:36.56 SS.reconfigure forSampling @09:33:36.59 SS.seek 25.00ml @09:33:38.48 Vacuum too high -- waiting up to 40s @09:33:38.96 Intake @48.0psia, vacuum from 20.0 to 24.0psi @09:33:38.98 SS.reconfigure forSampling @09:33:39.01 SS.seek 25.00ml @09:33:40.98 Vacuum too high -- waiting up to 40s @09:33:41.45 Intake @48.9psia, vacuum from 20.0 to 24.0psi @09:33:42.09 SS.reconfigure forSampling @09:33:42.12 SS.seek 25.00ml @09:33:44.79 Vacuum too high -- waiting up to 40s @09:33:45.31 SS.seek 25.00ml @09:33:46.98 Vacuum too high -- waiting up to 40s @09:33:47.47 Intake @48.2psia, vacuum from 20.0 to 24.0psi @09:33:47.49 SS.reconfigure forSampling @09:33:47.52 SS.seek 25.00ml @09:33:49.45 Vacuum too high -- waiting up to 40s @09:33:49.94 Intake @48.3psia, vacuum from 20.0 to 24.0psi @09:33:49.95 SS.reconfigure forSampling @09:33:49.99 SS.seek 25.00ml @09:33:51.61 Vacuum too high -- waiting up to 40s @09:33:52.10 Intake @48.2psia, vacuum from 20.0 to 24.0psi @09:33:52.11 SS.reconfigure forSampling @09:33:52.15 SS.seek 25.00ml @09:33:53.79 Vacuum too high -- waiting up to 40s @09:33:54.29 SS.seek 25.00ml @09:33:55.86 Vacuum too high -- waiting up to 40s @09:33:56.34 Intake @47.9psia, vacuum from 20.0 to 24.0psi @09:33:56.35 SS.reconfigure forSampling @09:33:56.39 SS.seek 25.00ml @09:33:57.98 Vacuum too high -- waiting up to 40s @09:33:58.46 Intake @48.3psia, vacuum from 20.0 to 24.0psi @09:33:58.48 SS.reconfigure forSampling @09:33:58.51 SS.seek 25.00ml @09:34:00.26 Vacuum too high -- waiting up to 40s @09:34:00.74 Intake @48.8psia, vacuum from 20.0 to 24.0psi @09:34:00.75 SS.reconfigure forSampling @09:34:00.79 SS.seek 25.00ml @09:34:02.11 Vacuum too high -- waiting up to 40s @09:34:02.59 Intake @48.0psia, vacuum from 20.0 to 24.0psi @09:34:02.60 SS.reconfigure forSampling @09:34:02.64 SS.seek 25.00ml @09:34:04.20 Vacuum too high -- waiting up to 40s @09:34:04.68 Intake @47.8psia, vacuum from 20.0 to 24.0psi @09:34:04.69 SS.reconfigure forSampling @09:34:04.73 SS.seek 25.00ml @09:34:06.20 Vacuum too high -- waiting up to 40s @09:34:06.69 Intake @47.5psia, vacuum from 20.0 to 24.0psi @09:34:06.70 SS.reconfigure forSampling @09:34:06.73 SS.seek 25.00ml @09:34:08.32 Vacuum too high -- waiting up to 40s @09:34:08.80 Intake @47.9psia, vacuum from 20.0 to 24.0psi @09:34:08.81 SS.reconfigure forSampling @09:34:08.84 SS.seek 25.00ml @09:34:10.26 Vacuum too high -- waiting up to 40s @09:34:10.74 Intake @47.8psia, vacuum from 20.0 to 24.0psi @09:34:10.75 SS.reconfigure forSampling @09:34:10.78 SS.seek 25.00ml @09:34:12.17 Vacuum too high -- waiting up to 40s @09:34:12.64 Intake @47.6psia, vacuum from 20.0 to 24.0psi @09:34:12.66 SS.reconfigure forSampling @09:34:12.69 SS.seek 25.00ml @09:34:14.14 Vacuum too high -- waiting up to 40s @09:34:14.61 Intake @47.7psia, vacuum from 20.0 to 24.0psi @09:34:14.63 SS.reconfigure forSampling @09:34:14.66 SS.seek 25.00ml @09:34:16.11 Vacuum too high -- waiting up to 40s @09:34:16.58 Intake @47.5psia, vacuum from 20.0 to 24.0psi @09:34:16.59 SS.reconfigure forSampling @09:34:16.62 SS.seek 25.00ml @09:34:18.20 Vacuum too high -- waiting up to 40s @09:34:18.90 Intake @48.4psia, vacuum from 20.0 to 24.0psi @09:34:18.91 SS.reconfigure forSampling @09:34:18.95 SS.seek 25.00ml @09:34:20.36 Vacuum too high -- waiting up to 40s @09:34:21.51 Intake @47.6psia, vacuum from 20.0 to 24.0psi @09:34:21.53 SS.reconfigure forSampling @09:34:21.56 SS.seek 25.00ml @09:34:23.51 Vacuum too high -- waiting up to 40s @09:34:24.00 Intake @47.2psia, vacuum from 20.0 to 24.0psi @09:34:24.02 SS.reconfigure forSampling @09:34:24.05 SS.seek 25.00ml @09:34:25.39 Vacuum too high -- waiting up to 40s @09:34:25.88 Intake @47.0psia, vacuum from 20.0 to 24.0psi @09:34:25.90 SS.reconfigure forSampling @09:34:25.93 SS.seek 25.00ml @09:34:27.45 Vacuum too high -- waiting up to 40s @09:34:27.93 Intake @47.3psia, vacuum from 20.0 to 24.0psi @09:34:27.95 SS.reconfigure forSampling @09:34:27.98 SS.seek 25.00ml @09:34:29.26 Vacuum too high -- waiting up to 40s @09:34:29.75 Intake @46.9psia, vacuum from 20.0 to 24.0psi @09:34:29.77 SS.reconfigure forSampling @09:34:29.80 SS.seek 25.00ml @09:34:31.26 Vacuum too high -- waiting up to 40s @09:34:31.73 Intake @47.3psia, vacuum from 20.0 to 24.0psi @09:34:31.75 SS.reconfigure forSampling @09:34:31.78 SS.seek 25.00ml @09:34:33.15 Vacuum too high -- waiting up to 40s @09:34:33.17 delay 30 seconds @09:34:33.64 Intake @47.6psia, vacuum from 20.0 to 24.0psi @09:34:33.65 SS.reconfigure forSampling @09:34:33.68 SS.seek 25.00ml @09:34:35.07 Vacuum too high -- waiting up to 40s @09:34:35.55 Intake @47.7psia, vacuum from 20.0 to 24.0psi @09:34:35.57 SS.reconfigure forSampling @09:34:35.60 SS.seek 25.00ml @09:34:36.82 Vacuum too high -- waiting up to 40s @09:34:37.31 Intake @47.7psia, vacuum from 20.0 to 24.0psi @09:34:37.33 SS.reconfigure forSampling @09:34:37.36 SS.seek 25.00ml @09:34:38.63 Vacuum too high -- waiting up to 40s @09:34:39.12 Intake @47.0psia, vacuum from 20.0 to 24.0psi @09:34:39.13 SS.reconfigure forSampling @09:34:39.17 SS.seek 25.00ml @09:34:40.51 Vacuum too high -- waiting up to 40s @09:34:40.99 Intake @46.6psia, vacuum from 20.0 to 24.0psi @09:34:41.01 SS.reconfigure forSampling @09:34:41.04 SS.seek 25.00ml @09:34:42.32 Vacuum too high -- waiting up to 40s @09:34:42.81 Intake @47.0psia, vacuum from 20.0 to 24.0psi @09:34:42.82 SS.reconfigure forSampling @09:34:42.85 SS.seek 25.00ml @09:34:44.23 Vacuum too high -- waiting up to 40s @09:34:44.92 Intake @48.3psia, vacuum from 20.0 to 24.0psi @09:34:44.94 SS.reconfigure forSampling @09:34:44.97 SS.seek 25.00ml @09:34:46.26 Vacuum too high -- waiting up to 40s @09:34:46.72 Intake @48.5psia, vacuum from 20.0 to 24.0psi @09:34:46.74 SS.reconfigure forSampling @09:34:46.77 SS.seek 25.00ml @09:34:47.82 Vacuum too high -- waiting up to 40s @09:34:48.30 Intake @47.5psia, vacuum from 20.0 to 24.0psi @09:34:48.31 SS.reconfigure forSampling @09:34:48.34 SS.seek 25.00ml @09:34:49.76 Vacuum too high -- waiting up to 40s @09:34:50.24 Intake @47.4psia, vacuum from 20.0 to 24.0psi @09:34:50.25 SS.reconfigure forSampling @09:34:50.29 SS.seek 25.00ml @09:34:51.57 Vacuum too high -- waiting up to 40s @09:34:52.05 Intake @47.5psia, vacuum from 20.0 to 24.0psi @09:34:52.06 SS.reconfigure forSampling @09:34:52.09 SS.seek 25.00ml @09:34:53.29 Vacuum too high -- waiting up to 40s @09:34:53.77 SS.seek 25.00ml @09:34:56.26 Vacuum too high -- waiting up to 40s @09:34:56.75 Intake @47.7psia, vacuum from 20.0 to 24.0psi @09:34:56.77 SS.reconfigure forSampling @09:34:56.80 SS.seek 25.00ml @09:34:57.95 Vacuum too high -- waiting up to 40s @09:34:58.43 Intake @47.8psia, vacuum from 20.0 to 24.0psi @09:34:58.44 SS.reconfigure forSampling @09:34:58.48 SS.seek 25.00ml @09:34:59.54 Vacuum too high -- waiting up to 40s @09:35:00.02 Intake @47.6psia, vacuum from 20.0 to 24.0psi @09:35:00.04 SS.reconfigure forSampling @09:35:00.07 SS.seek 25.00ml @09:35:01.32 Vacuum too high -- waiting up to 40s @09:35:01.81 Intake @47.4psia, vacuum from 20.0 to 24.0psi @09:35:01.83 SS.reconfigure forSampling @09:35:01.86 SS.seek 25.00ml @09:35:03.10 Vacuum too high -- waiting up to 40s @09:35:03.18 ATV.dial :waste,via => air3 @09:35:03.58 Intake @47.2psia, vacuum from 20.0 to 24.0psi @09:35:03.59 SS.reconfigure forSampling @09:35:03.63 SS.seek 25.00ml @09:35:04.73 Vacuum too high -- waiting up to 40s @09:35:04.78 AS.reconfigure ASconfig @09:35:04.81 AS.seek empty @09:35:05.22 Intake @47.0psia, vacuum from 20.0 to 24.0psi @09:35:05.24 SS.reconfigure forSampling @09:35:05.27 SS.seek 25.00ml @09:35:06.42 Vacuum too high -- waiting up to 40s @09:35:06.91 Intake @46.9psia, vacuum from 20.0 to 24.0psi @09:35:06.93 SS.reconfigure forSampling @09:35:06.96 SS.seek 25.00ml @09:35:08.17 Vacuum too high -- waiting up to 40s @09:35:08.66 Intake @46.7psia, vacuum from 20.0 to 24.0psi @09:35:08.67 SS.reconfigure forSampling @09:35:08.71 SS.seek 25.00ml @09:35:09.85 Vacuum too high -- waiting up to 40s @09:35:10.55 Intake @47.5psia, vacuum from 20.0 to 24.0psi @09:35:10.56 SS.reconfigure forSampling @09:35:10.59 SS.seek 25.00ml @09:35:11.95 Vacuum too high -- waiting up to 40s @09:35:12.43 Intake @47.9psia, vacuum from 20.0 to 24.0psi @09:35:12.44 SS.reconfigure forSampling @09:35:12.48 SS.seek 25.00ml @09:35:13.57 Vacuum too high -- waiting up to 40s @09:35:14.04 Intake @46.6psia, vacuum from 20.0 to 24.0psi @09:35:14.05 SS.reconfigure forSampling @09:35:14.08 SS.seek 25.00ml @09:35:15.35 Vacuum too high -- waiting up to 40s @09:35:16.05 Intake @46.7psia, vacuum from 20.0 to 24.0psi @09:35:16.06 SS.reconfigure forSampling @09:35:16.10 SS.seek 25.00ml @09:35:17.38 Vacuum too high -- waiting up to 40s @09:35:17.86 Intake @46.4psia, vacuum from 20.0 to 24.0psi @09:35:17.88 SS.reconfigure forSampling @09:35:17.91 SS.seek 25.00ml @09:35:19.05 Vacuum too high -- waiting up to 40s @09:35:19.53 Intake @46.3psia, vacuum from 20.0 to 24.0psi @09:35:19.54 SS.reconfigure forSampling @09:35:19.57 SS.seek 25.00ml @09:35:20.76 Vacuum too high -- waiting up to 40s @09:35:21.25 Intake @46.8psia, vacuum from 20.0 to 24.0psi @09:35:21.26 SS.reconfigure forSampling @09:35:21.29 SS.seek 25.00ml @09:35:22.30 Parking Bleach in LysateSource line and SPEcolumn @09:35:22.31 ATV.dial :air3 @09:35:22.38 Vacuum too high -- waiting up to 40s @09:35:22.90 AS.reconfigure ASslow1 @09:35:22.93 AS.seek 0.029ml @09:35:23.09 Intake @48.0psia, vacuum from 20.0 to 24.0psi @09:35:23.11 SS.reconfigure forSampling @09:35:23.14 SS.seek 25.00ml @09:35:25.60 Vacuum too high -- waiting up to 40s @09:35:26.09 Intake @47.5psia, vacuum from 20.0 to 24.0psi @09:35:26.11 SS.reconfigure forSampling @09:35:26.14 SS.seek 25.00ml @09:35:27.29 Vacuum too high -- waiting up to 40s @09:35:27.78 Intake @47.0psia, vacuum from 20.0 to 24.0psi @09:35:27.80 SS.reconfigure forSampling @09:35:27.83 SS.seek 25.00ml @09:35:28.88 Vacuum too high -- waiting up to 40s @09:35:29.38 Intake @46.8psia, vacuum from 20.0 to 24.0psi @09:35:29.40 SS.reconfigure forSampling @09:35:29.43 SS.seek 25.00ml @09:35:29.46 ATV.dial :ElutionWater @09:35:29.87 AS.seek 0.059ml @09:35:30.60 Vacuum too high -- waiting up to 40s @09:35:31.09 Intake @46.7psia, vacuum from 20.0 to 24.0psi @09:35:31.11 SS.reconfigure forSampling @09:35:31.14 SS.seek 25.00ml @09:35:32.54 Vacuum too high -- waiting up to 40s @09:35:33.25 Intake @47.6psia, vacuum from 20.0 to 24.0psi @09:35:33.27 SS.reconfigure forSampling @09:35:33.30 SS.seek 25.00ml @09:35:34.54 Vacuum too high -- waiting up to 40s @09:35:35.24 Intake @47.4psia, vacuum from 20.0 to 24.0psi @09:35:35.26 SS.reconfigure forSampling @09:35:35.29 SS.seek 25.00ml @09:35:35.77 Stroke completed -- waiting for pressure to equalize ... @09:35:36.27 ATV.dial :air3 @09:35:36.67 AS.seek 0.069ml @09:35:38.20 Intake.select :close @09:35:38.34 SSV.dial :exhaust @09:35:39.04 delay 2 seconds @09:35:39.14 Exhaust.select :open @09:35:39.24 SS.reconfigure SSexhale @09:35:39.28 SS.seek 0.00ml @09:35:41.05 ATV.dial :bleach @09:35:41.43 AS.seek 0.709ml @09:35:48.55 Exhaust.select :close @09:35:48.65 SSV.dial CBV @09:35:48.67 CBV.dial SSV @09:35:49.47 Allowing 185s for 25.0ml of remaining 850.0ml @09:35:49.48 Intake.select :open @09:35:51.62 Intake @47.1psia, vacuum from 20.0 to 24.0psi @09:35:51.64 SS.reconfigure forSampling @09:35:51.67 SS.seek 25.00ml @09:35:53.85 Vacuum too high -- waiting up to 40s @09:35:54.34 Intake @46.8psia, vacuum from 20.0 to 24.0psi @09:35:54.35 SS.reconfigure forSampling @09:35:54.39 SS.seek 25.00ml @09:35:55.66 Vacuum too high -- waiting up to 40s @09:35:56.14 Intake @46.3psia, vacuum from 20.0 to 24.0psi @09:35:56.15 SS.reconfigure forSampling @09:35:56.18 SS.seek 25.00ml @09:35:57.35 Vacuum too high -- waiting up to 40s @09:35:57.82 Intake @46.2psia, vacuum from 20.0 to 24.0psi @09:35:57.83 SS.reconfigure forSampling @09:35:57.87 SS.seek 25.00ml @09:35:58.98 Vacuum too high -- waiting up to 40s @09:35:59.47 Intake @46.4psia, vacuum from 20.0 to 24.0psi @09:35:59.48 SS.reconfigure forSampling @09:35:59.51 SS.seek 25.00ml @09:36:00.63 Vacuum too high -- waiting up to 40s @09:36:01.42 Intake @46.2psia, vacuum from 20.0 to 24.0psi @09:36:01.43 SS.reconfigure forSampling @09:36:01.46 SS.seek 25.00ml @09:36:02.85 Vacuum too high -- waiting up to 40s @09:36:03.53 Intake @47.3psia, vacuum from 20.0 to 24.0psi @09:36:03.55 SS.reconfigure forSampling @09:36:03.58 SS.seek 25.00ml @09:36:04.76 Vacuum too high -- waiting up to 40s @09:36:05.90 Intake @46.5psia, vacuum from 20.0 to 24.0psi @09:36:05.92 SS.reconfigure forSampling @09:36:05.95 SS.seek 25.00ml @09:36:07.66 Vacuum too high -- waiting up to 40s @09:36:08.15 Intake @45.8psia, vacuum from 20.0 to 24.0psi @09:36:08.16 SS.reconfigure forSampling @09:36:08.20 SS.seek 25.00ml @09:36:09.41 Vacuum too high -- waiting up to 40s @09:36:10.12 Intake @45.7psia, vacuum from 20.0 to 24.0psi @09:36:10.13 SS.reconfigure forSampling @09:36:10.17 SS.seek 25.00ml @09:36:11.44 Vacuum too high -- waiting up to 40s @09:36:12.14 Intake @47.2psia, vacuum from 20.0 to 24.0psi @09:36:12.16 SS.reconfigure forSampling @09:36:12.19 SS.seek 25.00ml @09:36:13.38 Vacuum too high -- waiting up to 40s @09:36:14.08 Intake @47.9psia, vacuum from 20.0 to 24.0psi @09:36:14.09 SS.reconfigure forSampling @09:36:14.13 SS.seek 25.00ml @09:36:15.26 Vacuum too high -- waiting up to 40s @09:36:15.74 Intake @48.0psia, vacuum from 20.0 to 24.0psi @09:36:15.75 SS.reconfigure forSampling @09:36:15.79 SS.seek 25.00ml @09:36:16.85 Vacuum too high -- waiting up to 40s @09:36:17.34 Intake @47.4psia, vacuum from 20.0 to 24.0psi @09:36:17.35 SS.reconfigure forSampling @09:36:17.38 SS.seek 25.00ml @09:36:18.44 Vacuum too high -- waiting up to 40s @09:36:18.91 Intake @47.3psia, vacuum from 20.0 to 24.0psi @09:36:18.92 SS.reconfigure forSampling @09:36:18.96 SS.seek 25.00ml @09:36:19.91 Vacuum too high -- waiting up to 40s @09:36:20.39 Intake @47.0psia, vacuum from 20.0 to 24.0psi @09:36:20.41 SS.reconfigure forSampling @09:36:20.44 SS.seek 25.00ml @09:36:21.47 Vacuum too high -- waiting up to 40s @09:36:21.96 Intake @46.9psia, vacuum from 20.0 to 24.0psi @09:36:21.97 SS.reconfigure forSampling @09:36:22.01 SS.seek 25.00ml @09:36:23.01 Vacuum too high -- waiting up to 40s @09:36:23.49 Intake @46.8psia, vacuum from 20.0 to 24.0psi @09:36:23.51 SS.reconfigure forSampling @09:36:23.54 SS.seek 25.00ml @09:36:24.54 Vacuum too high -- waiting up to 40s @09:36:25.03 Intake @46.7psia, vacuum from 20.0 to 24.0psi @09:36:25.04 SS.reconfigure forSampling @09:36:25.08 SS.seek 25.00ml @09:36:26.07 Vacuum too high -- waiting up to 40s @09:36:26.76 Intake @46.6psia, vacuum from 20.0 to 24.0psi @09:36:26.77 SS.reconfigure forSampling @09:36:26.80 SS.seek 25.00ml @09:36:28.07 Vacuum too high -- waiting up to 40s @09:36:28.76 Intake @47.9psia, vacuum from 20.0 to 24.0psi @09:36:28.77 SS.reconfigure forSampling @09:36:28.81 SS.seek 25.00ml @09:36:29.94 Vacuum too high -- waiting up to 40s @09:36:30.42 SS.seek 25.00ml @09:36:31.29 Vacuum too high -- waiting up to 40s @09:36:31.76 Intake @46.9psia, vacuum from 20.0 to 24.0psi @09:36:31.78 SS.reconfigure forSampling @09:36:31.81 SS.seek 25.00ml @09:36:32.82 Vacuum too high -- waiting up to 40s @09:36:33.50 Intake @47.0psia, vacuum from 20.0 to 24.0psi @09:36:33.51 SS.reconfigure forSampling @09:36:33.55 SS.seek 25.00ml @09:36:35.69 Vacuum too high -- waiting up to 40s @09:36:36.18 Intake @46.5psia, vacuum from 20.0 to 24.0psi @09:36:36.20 SS.reconfigure forSampling @09:36:36.23 SS.seek 25.00ml @09:36:37.22 Vacuum too high -- waiting up to 40s @09:36:37.93 Intake @46.6psia, vacuum from 20.0 to 24.0psi @09:36:37.94 SS.reconfigure forSampling @09:36:37.98 SS.seek 25.00ml @09:36:39.13 Vacuum too high -- waiting up to 40s @09:36:39.83 Intake @46.8psia, vacuum from 20.0 to 24.0psi @09:36:39.85 SS.reconfigure forSampling @09:36:39.88 SS.seek 25.00ml @09:36:41.16 Vacuum too high -- waiting up to 40s @09:36:41.87 Intake @47.3psia, vacuum from 20.0 to 24.0psi @09:36:41.89 SS.reconfigure forSampling @09:36:41.92 SS.seek 25.00ml @09:36:42.97 Vacuum too high -- waiting up to 40s @09:36:43.68 Intake @47.2psia, vacuum from 20.0 to 24.0psi @09:36:43.69 SS.reconfigure forSampling @09:36:43.73 SS.seek 25.00ml @09:36:44.91 Vacuum too high -- waiting up to 40s @09:36:45.39 Intake @46.8psia, vacuum from 20.0 to 24.0psi @09:36:45.40 SS.reconfigure forSampling @09:36:45.44 SS.seek 25.00ml @09:36:46.38 Vacuum too high -- waiting up to 40s @09:36:47.08 Intake @47.2psia, vacuum from 20.0 to 24.0psi @09:36:47.09 SS.reconfigure forSampling @09:36:47.13 SS.seek 25.00ml @09:36:48.22 Vacuum too high -- waiting up to 40s @09:36:48.93 Intake @47.4psia, vacuum from 20.0 to 24.0psi @09:36:48.94 SS.reconfigure forSampling @09:36:48.98 SS.seek 25.00ml @09:36:50.03 Vacuum too high -- waiting up to 40s @09:36:50.52 Intake @46.7psia, vacuum from 20.0 to 24.0psi @09:36:50.53 SS.reconfigure forSampling @09:36:50.57 SS.seek 25.00ml @09:36:51.57 Vacuum too high -- waiting up to 40s @09:36:52.27 Intake @46.9psia, vacuum from 20.0 to 24.0psi @09:36:52.28 SS.reconfigure forSampling @09:36:52.31 SS.seek 25.00ml @09:36:53.38 Vacuum too high -- waiting up to 40s @09:36:54.07 Intake @46.6psia, vacuum from 20.0 to 24.0psi @09:36:54.08 SS.reconfigure forSampling @09:36:54.12 SS.seek 25.00ml @09:36:55.22 Vacuum too high -- waiting up to 40s @09:36:55.90 Intake @47.2psia, vacuum from 20.0 to 24.0psi @09:36:55.91 SS.reconfigure forSampling @09:36:55.95 SS.seek 25.00ml @09:36:56.91 Vacuum too high -- waiting up to 40s @09:36:57.60 Intake @47.2psia, vacuum from 20.0 to 24.0psi @09:36:57.62 SS.reconfigure forSampling @09:36:57.65 SS.seek 25.00ml @09:36:58.78 Vacuum too high -- waiting up to 40s @09:36:59.48 SS.seek 25.00ml @09:37:00.50 Vacuum too high -- waiting up to 40s @09:37:01.20 Intake @47.8psia, vacuum from 20.0 to 24.0psi @09:37:01.21 SS.reconfigure forSampling @09:37:01.25 SS.seek 25.00ml @09:37:02.22 Vacuum too high -- waiting up to 40s @09:37:03.38 Intake @47.7psia, vacuum from 20.0 to 24.0psi @09:37:03.39 SS.reconfigure forSampling @09:37:03.43 SS.seek 25.00ml @09:37:04.85 Vacuum too high -- waiting up to 40s @09:37:05.34 Intake @47.2psia, vacuum from 20.0 to 24.0psi @09:37:05.36 SS.reconfigure forSampling @09:37:05.39 SS.seek 25.00ml @09:37:06.28 Vacuum too high -- waiting up to 40s @09:37:06.99 Intake @46.9psia, vacuum from 20.0 to 24.0psi @09:37:07.01 SS.reconfigure forSampling @09:37:07.04 SS.seek 25.00ml @09:37:08.19 Vacuum too high -- waiting up to 40s @09:37:08.90 Intake @46.9psia, vacuum from 20.0 to 24.0psi @09:37:08.92 SS.reconfigure forSampling @09:37:08.95 SS.seek 25.00ml @09:37:09.97 Vacuum too high -- waiting up to 40s @09:37:10.68 Intake @47.6psia, vacuum from 20.0 to 24.0psi @09:37:10.69 SS.reconfigure forSampling @09:37:10.73 SS.seek 25.00ml @09:37:11.69 Vacuum too high -- waiting up to 40s @09:37:12.40 Intake @48.4psia, vacuum from 20.0 to 24.0psi @09:37:12.42 SS.reconfigure forSampling @09:37:12.45 SS.seek 25.00ml @09:37:13.44 Vacuum too high -- waiting up to 40s @09:37:13.93 Intake @47.9psia, vacuum from 20.0 to 24.0psi @09:37:13.95 SS.reconfigure forSampling @09:37:13.98 SS.seek 25.00ml @09:37:14.88 Vacuum too high -- waiting up to 40s @09:37:15.36 Intake @47.2psia, vacuum from 20.0 to 24.0psi @09:37:15.38 SS.reconfigure forSampling @09:37:15.41 SS.seek 25.00ml @09:37:16.31 Vacuum too high -- waiting up to 40s @09:37:17.03 SS.seek 25.00ml @09:37:17.94 Vacuum too high -- waiting up to 40s @09:37:18.65 Intake @46.9psia, vacuum from 20.0 to 24.0psi @09:37:18.66 SS.reconfigure forSampling @09:37:18.69 SS.seek 25.00ml @09:37:19.93 Vacuum too high -- waiting up to 40s @09:37:20.41 Intake @47.0psia, vacuum from 20.0 to 24.0psi @09:37:20.42 SS.reconfigure forSampling @09:37:20.45 SS.seek 25.00ml @09:37:21.44 Vacuum too high -- waiting up to 40s @09:37:22.14 Intake @47.0psia, vacuum from 20.0 to 24.0psi @09:37:22.15 SS.reconfigure forSampling @09:37:22.19 SS.seek 25.00ml @09:37:23.22 Vacuum too high -- waiting up to 40s @09:37:23.90 Intake @47.3psia, vacuum from 20.0 to 24.0psi @09:37:23.92 SS.reconfigure forSampling @09:37:23.95 SS.seek 25.00ml @09:37:24.88 Vacuum too high -- waiting up to 40s @09:37:25.55 Intake @47.9psia, vacuum from 20.0 to 24.0psi @09:37:25.57 SS.reconfigure forSampling @09:37:25.60 SS.seek 25.00ml @09:37:26.66 Vacuum too high -- waiting up to 40s @09:37:27.36 Intake @47.8psia, vacuum from 20.0 to 24.0psi @09:37:27.37 SS.reconfigure forSampling @09:37:27.40 SS.seek 25.00ml @09:37:28.31 Vacuum too high -- waiting up to 40s @09:37:28.80 Intake @47.2psia, vacuum from 20.0 to 24.0psi @09:37:28.81 SS.reconfigure forSampling @09:37:28.85 SS.seek 25.00ml @09:37:29.75 Vacuum too high -- waiting up to 40s @09:37:30.77 Intake @47.0psia, vacuum from 20.0 to 24.0psi @09:37:30.79 SS.reconfigure forSampling @09:37:30.82 SS.seek 25.00ml @09:37:32.00 Vacuum too high -- waiting up to 40s @09:37:32.71 Intake @46.8psia, vacuum from 20.0 to 24.0psi @09:37:32.73 SS.reconfigure forSampling @09:37:32.76 SS.seek 25.00ml @09:37:33.72 Vacuum too high -- waiting up to 40s @09:37:34.44 Intake @46.9psia, vacuum from 20.0 to 24.0psi @09:37:34.45 SS.reconfigure forSampling @09:37:34.49 SS.seek 25.00ml @09:37:35.44 Vacuum too high -- waiting up to 40s @09:37:36.15 Intake @47.8psia, vacuum from 20.0 to 24.0psi @09:37:36.17 SS.reconfigure forSampling @09:37:36.20 SS.seek 25.00ml @09:37:37.13 Vacuum too high -- waiting up to 40s @09:37:37.84 Intake @47.7psia, vacuum from 20.0 to 24.0psi @09:37:37.85 SS.reconfigure forSampling @09:37:37.89 SS.seek 25.00ml @09:37:38.97 Vacuum too high -- waiting up to 40s @09:37:39.46 Intake @47.0psia, vacuum from 20.0 to 24.0psi @09:37:39.47 SS.reconfigure forSampling @09:37:39.51 SS.seek 25.00ml @09:37:40.34 Vacuum too high -- waiting up to 40s @09:37:41.05 Intake @46.8psia, vacuum from 20.0 to 24.0psi @09:37:41.06 SS.reconfigure forSampling @09:37:41.09 SS.seek 25.00ml @09:37:42.03 Vacuum too high -- waiting up to 40s @09:37:42.73 Intake @47.0psia, vacuum from 20.0 to 24.0psi @09:37:42.75 SS.reconfigure forSampling @09:37:42.78 SS.seek 25.00ml @09:37:43.69 Vacuum too high -- waiting up to 40s @09:37:44.40 Intake @47.6psia, vacuum from 20.0 to 24.0psi @09:37:44.41 SS.reconfigure forSampling @09:37:44.45 SS.seek 25.00ml @09:37:45.44 Vacuum too high -- waiting up to 40s @09:37:46.13 Intake @47.0psia, vacuum from 20.0 to 24.0psi @09:37:46.15 SS.reconfigure forSampling @09:37:46.18 SS.seek 25.00ml @09:37:47.19 Vacuum too high -- waiting up to 40s @09:37:47.89 Intake @46.9psia, vacuum from 20.0 to 24.0psi @09:37:47.90 SS.reconfigure forSampling @09:37:47.94 SS.seek 25.00ml @09:37:48.84 Vacuum too high -- waiting up to 40s @09:37:49.55 Intake @46.9psia, vacuum from 20.0 to 24.0psi @09:37:49.56 SS.reconfigure forSampling @09:37:49.60 SS.seek 25.00ml @09:37:50.53 Vacuum too high -- waiting up to 40s @09:37:51.23 Intake @47.3psia, vacuum from 20.0 to 24.0psi @09:37:51.24 SS.reconfigure forSampling @09:37:51.28 SS.seek 25.00ml @09:37:52.19 Vacuum too high -- waiting up to 40s @09:37:52.22 delay 3 seconds @09:37:52.89 Intake @46.9psia, vacuum from 20.0 to 24.0psi @09:37:52.90 SS.reconfigure forSampling @09:37:52.94 SS.seek 25.00ml @09:37:53.94 Vacuum too high -- waiting up to 40s @09:37:54.63 Intake @46.5psia, vacuum from 20.0 to 24.0psi @09:37:54.65 SS.reconfigure forSampling @09:37:54.68 SS.seek 25.00ml @09:37:55.23 ATV.dial :air3 @09:37:55.59 delay 3 seconds @09:37:55.66 Vacuum too high -- waiting up to 40s @09:37:56.59 Intake @46.4psia, vacuum from 20.0 to 24.0psi @09:37:56.61 SS.reconfigure forSampling @09:37:56.64 SS.seek 25.00ml @09:37:57.88 Vacuum too high -- waiting up to 40s @09:37:58.59 Intake @46.7psia, vacuum from 20.0 to 24.0psi @09:37:58.61 SS.reconfigure forSampling @09:37:58.65 SS.seek 25.00ml @09:37:58.73 AS.seek 0.734ml @09:37:59.78 Vacuum too high -- waiting up to 40s @09:38:00.48 Intake @47.6psia, vacuum from 20.0 to 24.0psi @09:38:00.49 SS.reconfigure forSampling @09:38:00.53 SS.seek 25.00ml @09:38:01.56 Vacuum too high -- waiting up to 40s @09:38:02.27 Intake @47.1psia, vacuum from 20.0 to 24.0psi @09:38:02.29 SS.reconfigure forSampling @09:38:02.32 SS.seek 25.00ml @09:38:03.25 Vacuum too high -- waiting up to 40s @09:38:03.96 Intake @46.6psia, vacuum from 20.0 to 24.0psi @09:38:03.98 SS.reconfigure forSampling @09:38:04.01 SS.seek 25.00ml @09:38:04.09 delay 3 seconds @09:38:04.97 Vacuum too high -- waiting up to 40s @09:38:05.68 SS.seek 25.00ml @09:38:06.59 Vacuum too high -- waiting up to 40s @09:38:07.10 ATV.dial :bleach @09:38:07.30 SS.seek 25.00ml @09:38:07.49 AS.seek 0.719ml @09:38:08.25 Vacuum too high -- waiting up to 40s @09:38:08.97 Intake @46.7psia, vacuum from 20.0 to 24.0psi @09:38:08.98 SS.reconfigure forSampling @09:38:09.02 SS.seek 25.00ml @09:38:10.00 Vacuum too high -- waiting up to 40s @09:38:10.71 Intake @47.0psia, vacuum from 20.0 to 24.0psi @09:38:10.72 SS.reconfigure forSampling @09:38:10.76 SS.seek 25.00ml @09:38:10.81 delay 3 seconds @09:38:11.59 Vacuum too high -- waiting up to 40s @09:38:12.29 Intake @47.5psia, vacuum from 20.0 to 24.0psi @09:38:12.30 SS.reconfigure forSampling @09:38:12.34 SS.seek 25.00ml @09:38:13.28 Vacuum too high -- waiting up to 40s @09:38:13.82 ATV.dial :AIV @09:38:14.00 SS.seek 25.00ml @09:38:14.91 Vacuum too high -- waiting up to 40s @09:38:15.04 AS.reconfigure ASconfig @09:38:15.08 AS.seek 0.015ml @09:38:15.47 Intake @47.1psia, vacuum from 20.0 to 24.0psi @09:38:15.49 SS.reconfigure forSampling @09:38:15.52 SS.seek 25.00ml @09:38:16.41 Vacuum too high -- waiting up to 40s @09:38:17.12 Intake @46.8psia, vacuum from 20.0 to 24.0psi @09:38:17.14 SS.reconfigure forSampling @09:38:17.17 SS.seek 25.00ml @09:38:18.09 Vacuum too high -- waiting up to 40s @09:38:18.79 Intake @46.6psia, vacuum from 20.0 to 24.0psi @09:38:18.80 SS.reconfigure forSampling @09:38:18.84 SS.seek 25.00ml @09:38:19.84 Vacuum too high -- waiting up to 40s @09:38:21.22 Intake @47.0psia, vacuum from 20.0 to 24.0psi @09:38:21.23 SS.reconfigure forSampling @09:38:21.27 SS.seek 25.00ml @09:38:22.78 Vacuum too high -- waiting up to 40s @09:38:23.49 Intake @47.1psia, vacuum from 20.0 to 24.0psi @09:38:23.51 SS.reconfigure forSampling @09:38:23.54 SS.seek 25.00ml @09:38:24.47 Vacuum too high -- waiting up to 40s @09:38:24.96 Intake @46.3psia, vacuum from 20.0 to 24.0psi @09:38:24.98 SS.reconfigure forSampling @09:38:25.01 SS.seek 25.00ml @09:38:25.81 Vacuum too high -- waiting up to 40s @09:38:26.50 Intake @46.2psia, vacuum from 20.0 to 24.0psi @09:38:26.52 SS.reconfigure forSampling @09:38:26.55 SS.seek 25.00ml @09:38:27.40 Vacuum too high -- waiting up to 40s @09:38:28.12 SS.seek 25.00ml @09:38:28.90 Vacuum too high -- waiting up to 40s @09:38:29.61 Intake @46.6psia, vacuum from 20.0 to 24.0psi @09:38:29.63 SS.reconfigure forSampling @09:38:29.66 SS.seek 25.00ml @09:38:30.56 Vacuum too high -- waiting up to 40s @09:38:31.26 Intake @46.8psia, vacuum from 20.0 to 24.0psi @09:38:31.28 SS.reconfigure forSampling @09:38:31.31 SS.seek 25.00ml @09:38:32.22 Vacuum too high -- waiting up to 40s @09:38:32.93 SS.seek 25.00ml @09:38:33.84 Vacuum too high -- waiting up to 40s @09:38:34.54 Intake @46.6psia, vacuum from 20.0 to 24.0psi @09:38:34.55 SS.reconfigure forSampling @09:38:34.59 SS.seek 25.00ml @09:38:35.40 Vacuum too high -- waiting up to 40s @09:38:36.10 Intake @46.4psia, vacuum from 20.0 to 24.0psi @09:38:36.11 SS.reconfigure forSampling @09:38:36.15 SS.seek 25.00ml @09:38:37.03 Vacuum too high -- waiting up to 40s @09:38:37.74 Intake @46.2psia, vacuum from 20.0 to 24.0psi @09:38:37.75 SS.reconfigure forSampling @09:38:37.78 SS.seek 25.00ml @09:38:38.75 Vacuum too high -- waiting up to 40s @09:38:39.45 Intake @46.4psia, vacuum from 20.0 to 24.0psi @09:38:39.46 SS.reconfigure forSampling @09:38:39.50 SS.seek 25.00ml @09:38:40.40 Vacuum too high -- waiting up to 40s @09:38:41.11 Intake @46.2psia, vacuum from 20.0 to 24.0psi @09:38:41.12 SS.reconfigure forSampling @09:38:41.15 SS.seek 25.00ml @09:38:42.12 Vacuum too high -- waiting up to 40s @09:38:42.83 Intake @46.3psia, vacuum from 20.0 to 24.0psi @09:38:42.84 SS.reconfigure forSampling @09:38:42.87 SS.seek 25.00ml @09:38:43.65 Vacuum too high -- waiting up to 40s @09:38:44.35 Intake @46.1psia, vacuum from 20.0 to 24.0psi @09:38:44.36 SS.reconfigure forSampling @09:38:44.40 SS.seek 25.00ml @09:38:45.31 Vacuum too high -- waiting up to 40s @09:38:46.00 Intake @45.6psia, vacuum from 20.0 to 24.0psi @09:38:46.01 SS.reconfigure forSampling @09:38:46.72 SS.seek 25.00ml @09:38:48.31 Vacuum too high -- waiting up to 40s @09:38:49.03 SS.seek 25.00ml @09:38:49.87 Vacuum too high -- waiting up to 40s @09:38:50.58 Intake @45.4psia, vacuum from 20.0 to 24.0psi @09:38:50.60 SS.reconfigure forSampling @09:38:50.63 SS.seek 25.00ml @09:38:51.50 Vacuum too high -- waiting up to 40s @09:38:52.21 Intake @45.0psia, vacuum from 20.0 to 24.0psi @09:38:52.23 SS.reconfigure forSampling @09:38:52.26 SS.seek 25.00ml @09:38:53.22 Vacuum too high -- waiting up to 40s @09:38:54.16 Intake @46.6psia, vacuum from 20.0 to 24.0psi @09:38:54.18 SS.reconfigure forSampling @09:38:54.21 SS.seek 25.00ml @09:38:54.49 SS.reconfigure SSconfig @09:38:54.52 SS.hold @09:38:54.69 Intake.select :close @09:38:54.84 ShallowSampler::Clogged in WCR -- progress too slow after 171.3ml @09:38:54.85 SSV.dial :exhaust @09:38:55.65 Exhaust.select :open @09:38:55.75 SS.reconfigure SSexhale @09:38:55.79 SS.seek 0.00ml @09:38:56.42 ATV.dial :waste @09:38:57.78 AS.seek empty @09:38:58.98 ATV.dial :air3 @09:38:59.57 AS.reconfigure ASslow1 @09:38:59.60 AS.seek 0.009ml @09:39:01.97 delay 2 seconds @09:39:03.51 Exhaust.select :close @09:39:03.62 Sampled 171.3ml @09:39:03.63 collectSample: Complete @09:39:03.64 Drying puck before fixing for 04:00.00 @09:39:03.65 CBV.dial :waste2b @09:39:03.98 ATV.dial :bleach @09:39:04.36 AS.seek 0.579ml @09:39:04.43 delay 1:00 @09:39:06.61 Sleepy.power :sampler,:OFF @09:40:04.43 CSV.dial :air @09:40:05.09 CS.reconfigure CSfast @09:40:05.12 CS.seek 10.000ml @09:40:28.95 CBV.dial between CSV and waste2b @09:40:29.26 Pumping wcr puck from tube 3 up to 55psi... @09:40:29.31 CBV.dial SSV @09:40:30.74 CBV.dial between CSV and waste2b @09:40:31.69 CSV.dial between air and CTV @09:40:31.99 CS.seek 6.708ml @09:40:40.37 CTV.dial CSV,avoiding => air @09:40:40.39 CSV.dial CTV @09:40:41.13 CS.reconfigure CSpressurize @09:40:41.16 CS.seek 0.000ml @09:40:58.73 CSV.dial :air @09:40:59.24 CS.reconfigure CSfast @09:40:59.27 CS.seek 10.000ml @09:41:00.81 delay 3 seconds @09:41:03.82 ATV.dial :air3 @09:41:04.15 delay 3 seconds @09:41:07.21 AS.seek 0.604ml @09:41:12.54 delay 3 seconds @09:41:15.55 ATV.dial :bleach @09:41:16.03 AS.seek 0.589ml @09:41:19.34 delay 3 seconds @09:41:22.35 ATV.dial :ASV @09:41:23.30 CSV.dial between air and CTV @09:41:23.35 ASV.dial :wasteR @09:41:23.88 AS.seek 0.000ml @09:41:24.29 CS.seek 2.385ml @09:41:42.40 CTV.dial CSV,avoiding => air @09:41:42.41 CSV.dial CTV @09:41:42.79 CS.reconfigure CSpressurize @09:41:42.82 CS.seek 0.000ml @09:41:45.78 CBV.dial :waste2b @09:41:46.10 CS.reconfigure CSconfig @09:41:46.13 CS.seek empty @09:41:50.45 delay 1:00 @09:42:50.47 CTV.dial :air,avoiding => intake @09:42:51.75 Fix #1 of 2 (with rnal) for 02:00.00 @09:42:51.76 CS Pulling 2ml of rnal CSV.dial CSR @09:42:52.19 CSR.select :rnal @09:42:52.29 CS.seek 2.000ml @09:42:58.17 delay 5 seconds @09:43:03.80 CSR.select :air @09:43:03.89 CS.reconfigure CSfast @09:43:03.93 CS.seek 10.000ml @09:43:23.20 CBV.dial between CSV and waste2b @09:43:23.53 Pumping wcr puck from tube 3 up to 55psi... @09:43:23.57 CBV.dial SSV @09:43:24.30 delay 1:00 @09:43:25.01 CBV.dial between CSV and waste2b @09:43:25.96 CSV.dial between air and CTV @09:43:26.62 CS.seek 6.732ml @09:43:34.96 CTV.dial CSV,avoiding => air @09:43:34.98 CSV.dial CTV @09:43:36.32 CS.reconfigure CSpressurize @09:43:36.35 CS.seek 0.000ml @09:43:53.50 delay 2:00 @09:44:24.31 AFV.select :carrierSolution @09:44:24.40 delay 2 seconds @09:44:26.41 AS.reconfigure ASconfig @09:44:26.44 AS.seek 1.000ml @09:45:25.01 delay 1 second @09:45:26.02 AFV.select :holdingCoil @09:45:26.11 delay 1 second @09:45:27.12 ATV.dial :ASV @09:45:27.13 ASV.dial :wasteR @09:45:27.14 AS.reconfigure ASslow1 @09:45:27.17 AS.seek 0.000ml @09:45:53.51 CBV.dial :waste2b @09:45:53.83 CS.reconfigure CSconfig @09:45:53.86 CS.seek empty @09:45:54.92 delay 1:00 @09:46:54.93 CTV.dial :air,avoiding => intake @09:46:56.20 Fix #2 of 2 (with rnal) for 02:00.00 @09:46:56.21 CS Pulling 2ml of rnal CSV.dial CSR @09:46:57.12 CSR.select :rnal @09:46:57.22 CS.seek 2.000ml @09:47:03.13 delay 5 seconds @09:47:08.14 CSR.select :air @09:47:08.23 CS.reconfigure CSfast @09:47:08.27 CS.seek 10.000ml @09:47:27.38 CBV.dial between CSV and waste2b @09:47:27.70 Pumping wcr puck from tube 3 up to 55psi... @09:47:27.74 CBV.dial SSV @09:47:29.17 CBV.dial between CSV and waste2b @09:47:30.12 CSV.dial between air and CTV @09:47:30.78 CS.seek 6.571ml @09:47:39.51 CTV.dial CSV,avoiding => air @09:47:39.53 CSV.dial CTV @09:47:40.83 CS.reconfigure CSpressurize @09:47:40.86 CS.seek 0.000ml @09:47:57.62 delay 2:00 @09:48:51.44 delay 20 seconds @09:49:11.45 ATV.dial :air3 @09:49:12.56 AS.seek 0.590ml @09:49:57.63 CBV.dial :waste2b @09:49:57.96 CS.reconfigure CSconfig @09:49:57.99 CS.seek empty @09:49:59.00 delay 1:00 @09:50:59.01 CTV.dial :air,avoiding => intake @09:51:00.33 Storing wcr puck in Collection Clamp to tube 2 @09:51:00.46 SC.reconfigure SCtight @09:51:00.49 SC.seek 2 @09:51:02.69 SC.reconfigure SCloose @09:51:02.86 Elbow.reconfigure TightElbow @09:51:02.90 Elbow.seek 2 @09:51:04.52 Forearm.reconfigure TightForearm @09:51:04.56 Forearm.seek 2 + 800 counts @09:51:05.52 SE.reconfigure SEpick @09:51:05.57 SE.seek beamBreak @09:51:08.40 SE.configure SEconfig @09:51:08.52 Elbow.reconfigure LimpElbow @09:51:08.55 Forearm.reconfigure LooseForearm @09:51:08.59 Forearm.seek 2 @09:51:08.84 Elbow.hold @09:51:08.90 Elbow.reconfigure TightElbow @09:51:08.98 SE.seek 3.04 Pucks below Top Plate @09:51:09.30 Forearm.coast @09:51:09.33 Forearm.seek 2 + 315 counts @09:51:09.56 Forearm.reconfigure TightForearm @09:51:09.59 Forearm.seek 2 + 800 counts @09:51:09.76 SE.seek 4.04 Pucks below Top Plate @09:51:10.90 Forearm.reconfigure LooseForearm @09:51:10.94 Forearm.seek retract @09:51:11.87 Elbow.seek CC @09:51:13.17 delay 3 seconds @09:51:13.47 Elbow.reconfigure LooseElbow @09:51:13.55 Forearm.seek CC @09:51:14.55 CC.seek sealed @09:51:15.50 Hand.grip @09:51:16.18 ATV.dial :ASV @09:51:16.23 CC.seek open @09:51:17.36 ASV.dial :wasteR @09:51:17.42 AS.seek 0.090ml @09:51:19.60 Forearm.seek retract @09:51:20.57 Elbow.reconfigure TightElbow @09:51:20.60 Elbow.seek 2 @09:51:22.18 Elbow.reconfigure LimpElbow @09:51:22.23 Forearm.seek 2 @09:51:23.20 Elbow.hold @09:51:23.26 Elbow.reconfigure TightElbow @09:51:23.30 Hand.release @09:51:24.16 Forearm.coast @09:51:24.19 Forearm.seek 2 + 342 counts @09:51:24.44 Forearm.reconfigure TightForearm @09:51:24.47 Forearm.seek 2 + 800 counts @09:51:24.80 SE.seek down @09:51:25.90 Forearm.reconfigure LooseForearm @09:51:25.94 Forearm.seek retract @09:51:27.13 SE.coast @09:51:27.16 SC.coast @09:51:27.19 Starting finalPurge @09:51:27.20 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @09:51:27.35 Elbow.seek FlushPuck::Garage @09:51:29.12 Elbow.reconfigure LooseElbow @09:51:29.20 Forearm.seek garage @09:51:30.23 Hand.grip @09:51:31.05 Forearm.seek retract @09:51:32.05 Elbow.reconfigure TightElbow @09:51:32.08 Elbow.seek CC @09:51:34.91 Elbow.reconfigure LooseElbow @09:51:35.00 Forearm.reconfigure TightForearm @09:51:35.03 Forearm.seek CC @09:51:36.11 CC.seek closed @09:51:39.87 Collection Clamp onSpring/offSpring for 4/21 samples, on/off torque 2.04 @09:51:39.92 Hand.release @09:51:40.75 Forearm.reconfigure LooseForearm @09:51:40.78 Forearm.seek retract @09:51:41.71 Flushing the Collection Valve::Manifold @09:51:41.81 CS Pulling 5ml of flush CSV.dial CSR @09:51:42.23 CSR.select :flush @09:51:42.33 CS.seek 5.000ml @09:51:55.90 delay 5 seconds @09:52:00.92 CSR.select :air @09:52:01.06 CS.reconfigure CSfast @09:52:01.77 CS.seek 7.500ml @09:52:08.34 CSV.dial :waste @09:52:09.60 CS.reconfigure CSconfig @09:52:09.63 CS.seek 0.000ml @09:52:29.43 Flushing lower manifold's waste1 port @09:52:29.52 CSV.dial :flush @09:52:29.97 CS.reconfigure CSfast @09:52:30.00 CS.seek 5.000ml @09:52:42.34 delay 5 seconds @09:52:47.35 CSV.dial :air @09:52:47.82 CS.seek 7.000ml @09:52:53.30 CSV.dial CTV @09:52:53.32
CTV.dial CSV @09:52:54.65 CBV.dial :waste1 @09:52:55.41 CS.seek 0.000ml @09:52:59.65 delay 30 seconds @09:53:12.15
Flushing lower manifold's waste2b port @09:53:12.24 CSV.dial :flush @09:53:13.05 CS.seek 5.000ml @09:53:25.36 delay 5 seconds @09:53:29.66 ATV.dial :extSrc @09:53:30.17 delay 3 seconds @09:53:30.37
CSV.dial :air @09:53:30.91 CS.seek 7.000ml @09:53:33.18 ATV.dial :ElutionWater @09:53:34.11 AS.seek 0.120ml @09:53:36.36 CSV.dial CTV @09:53:36.38
CTV.dial CSV @09:53:37.05 CBV.dial :waste2b @09:53:37.80 CS.seek 0.000ml @09:53:40.50 ATV.dial :air3 @09:53:40.88 AS.seek 0.130ml @09:53:43.20 delay 2 seconds @09:53:45.21 ATV.dial :bleach @09:53:45.59 AS.seek 0.230ml @09:53:54.33
Flushing upper manifold @09:53:54.42 CSV.dial :flush @09:53:55.14 CS.seek 5.000ml @09:54:06.28 delay 3 seconds @09:54:07.39
delay 5 seconds @09:54:09.29 ATV.dial :air3 @09:54:09.64 delay 3 seconds @09:54:12.40
CSV.dial :air @09:54:12.70 AS.seek 0.255ml @09:54:12.96
CS.seek 7.000ml @09:54:18.06 delay 3 seconds @09:54:18.33 CSV.dial CBV @09:54:18.35
CBV.dial CSV @09:54:19.50 CTV.dial :air @09:54:20.78 CS.seek 0.000ml @09:54:21.07 ATV.dial :bleach @09:54:21.47 AS.seek 0.240ml @09:54:24.75 delay 3 seconds @09:54:27.76 ATV.dial :ASV @09:54:28.64 ASV.dial :SPEdelivery @09:54:29.10 AFV.select :carrierSolution @09:54:29.20 delay 2 seconds @09:54:31.21 AS.reconfigure ASconfig @09:54:31.24 AS.seek 0.500ml @09:54:37.52
delay 7 seconds @09:54:44.53 CS.seek 3.000ml @09:54:46.67 delay 1 second @09:54:47.68 AFV.select :holdingCoil @09:54:47.82 AS.reconfigure ASslow1 @09:54:47.85 AS.seek 0.170ml @09:54:52.24
CSV.dial :waste @09:54:53.15 CS.seek 0.000ml @09:55:00.85 CSV.dial :flush @09:55:01.51 CS.seek 3.000ml @09:55:09.20 delay 5 seconds @09:55:14.21 CSV.dial :air @09:55:14.77 CS.seek 5.000ml @09:55:20.20 CSV.dial :air @09:55:20.22 CS.seek 0.000ml @09:55:32.36 delay 7 seconds @09:55:39.37 CS.seek 3.000ml @09:55:46.95 CSV.dial :waste @09:55:47.74 CS.seek 0.000ml @09:55:55.40 Disinfecting external Intake with mfbkill @09:55:55.47 SPE.hold 2:46:40,95.0C @09:55:55.49 delay 3:00 @09:55:55.58
CSV.dial CSR @09:55:56.97 CSR.select :mfbkill @09:55:57.07 CS.seek 8.000ml @09:56:17.01 delay 5 seconds @09:56:22.02 CSR.select :air @09:56:22.11 CS.seek 10.000ml @09:56:27.70 CSV.dial CBV @09:56:27.72
CBV.dial CSV @09:56:29.14 CTV.dial :intake @09:56:29.86 Intake.select :open @09:56:29.95 CS.seek 2.000ml @09:56:49.86 Intake.select :close @09:56:49.95 CTV.dial :air @09:56:51.33 CS.reconfigure CSconfig @09:56:51.36 CS.seek 0.000ml @09:56:57.48 Flushing lower manifold's waste1 port @09:56:57.49 CS Pulling 4ml of mfbkill @09:56:57.50 CSV.dial CSR @09:56:59.18 CSR.select :mfbkill @09:56:59.28 CS.seek 4.000ml @09:57:10.20 delay 5 seconds @09:57:15.21 CSR.select :air @09:57:15.30 CS.reconfigure CSfast @09:57:15.34 CS.seek 10.000ml @09:57:30.36 CSV.dial CTV @09:57:30.38
CTV.dial CSV @09:57:31.74 CBV.dial :waste1 @09:57:32.21 CS.seek 0.000ml @09:57:56.51 Flushing lower manifold's waste2b port @09:57:56.52 CS Pulling 4ml of mfbkill @09:57:56.53 CSV.dial CSR @09:57:57.04 CSR.select :mfbkill @09:57:57.14 CS.reconfigure CSconfig @09:57:57.18 CS.seek 4.000ml @09:58:08.16 delay 5 seconds @09:58:13.17 CSR.select :air @09:58:13.26 CS.reconfigure CSfast @09:58:13.30 CS.seek 10.000ml @09:58:28.33 CSV.dial CTV @09:58:28.35
CTV.dial CSV @09:58:28.88 CBV.dial :waste2b @09:58:29.64 CS.seek 0.000ml @09:58:53.88 Killing upper manifold @09:58:53.89 CS Pulling 4ml of mfbkill @09:58:53.90 CSV.dial CSR @09:58:54.41 CSR.select :mfbkill @09:58:54.51 CS.reconfigure CSconfig @09:58:54.55 CS.seek 4.000ml @09:58:55.51 AFV.select :carrierSolution @09:58:55.60 delay 2 seconds @09:58:57.61 AS.reconfigure ASconfig @09:58:57.64 AS.seek 1.000ml @09:59:05.54
delay 5 seconds @09:59:10.55 CSR.select :air @09:59:10.64 CS.reconfigure CSfast @09:59:10.68 CS.seek 10.000ml @09:59:25.50 CSV.dial CBV @09:59:25.52
CBV.dial CSV @09:59:26.57 CTV.dial :air @09:59:27.92 CS.seek 0.000ml @09:59:46.30 delay 1 second @09:59:47.31 AFV.select :holdingCoil @09:59:47.40 delay 1 second @09:59:48.41 ASV.dial :wasteD @09:59:49.71 AS.seek 0.000ml @09:59:51.86
delay 7 seconds @09:59:58.87 CS.seek 3.000ml @10:00:06.67 CSV.dial :waste @10:00:07.32 CS.seek 0.000ml @10:00:15.11 Flushing upper manifold @10:00:15.21 CSV.dial :flush @10:00:15.74 CS.seek 5.000ml @10:00:28.26 delay 5 seconds @10:00:33.27 CSV.dial :air @10:00:33.75 CS.seek 7.000ml @10:00:39.17 CSV.dial CBV @10:00:39.19
CBV.dial CSV @10:00:40.40 CTV.dial :air @10:00:40.42 CS.seek 0.000ml @10:00:48.32 Priming Eluate @10:00:48.33 AFV.select :carrierSolution @10:00:48.42 delay 2 seconds @10:00:50.43 AS.seek 0.500ml @10:00:57.35
delay 7 seconds @10:01:04.36 CS.seek 3.000ml @10:01:12.20 CSV.dial :waste @10:01:12.71 CS.seek 0.000ml @10:01:19.87 delay 1 second @10:01:20.53
CSV.dial :flush @10:01:20.88 AFV.select :holdingCoil @10:01:20.97
CS.seek 3.000ml @10:01:21.00 ATV.dial ASV @10:01:21.01 ASV.dial :SPErecovery @10:01:21.51 Swish #1 of 2 @10:01:21.56 AS.reconfigure ASslow0 @10:01:21.60 AS.seek 0.600ml @10:01:28.70
delay 5 seconds @10:01:32.14 AS.seek 0.500ml @10:01:33.71
CSV.dial :air @10:01:34.18 CS.seek 5.000ml @10:01:39.70 CSV.dial :air @10:01:39.72 CS.seek 0.000ml @10:01:42.62 Swish #2 of 2 @10:01:42.68 AS.seek 0.600ml @10:01:51.95
delay 7 seconds @10:01:53.23 AS.seek 0.500ml @10:01:58.96
CS.seek 3.000ml @10:02:03.73 delay 3 seconds @10:02:06.66
CSV.dial :waste @10:02:06.79 AS.reconfigure ASslow1 @10:02:06.82 AS.seek 0.676ml @10:02:07.47
CS.seek 0.000ml @10:02:15.16 Flushing lower manifold's waste1 port @10:02:15.25 CSV.dial :flush @10:02:16.38 CS.seek 5.000ml @10:02:28.60 delay 5 seconds @10:02:33.61 CSV.dial :air @10:02:34.10 CS.seek 6.999ml @10:02:39.51 CSV.dial CTV @10:02:39.53
CTV.dial CSV @10:02:40.86 CBV.dial :waste1 @10:02:41.33 CS.seek 0.000ml @10:02:42.99 delay 3 seconds @10:02:46.00 Eluate primed @10:02:46.01 ATV.dial :waste2 @10:02:46.53 AS.reconfigure ASnorm @10:02:46.56 AS.seek 0.000ml @10:02:58.04
Flushing lower manifold's waste2b port @10:02:58.13 CSV.dial :flush @10:02:58.94 CS.seek 5.000ml @10:03:11.22 delay 5 seconds @10:03:16.23 CSV.dial :air @10:03:16.77 CS.seek 7.000ml @10:03:21.43 SPE.coast @10:03:21.50 ASV.dial :SPErecovery @10:03:21.52 Setting up PCR for HF183em -- writing /var/log/esp/HF183em14mar1210h194ml.pcr @10:03:21.53 Building PCR Slug from ASV with MM2 mix, PP4 primer @10:03:21.54 Priming with carrier @10:03:21.55 ATV.dial ARV @10:03:22.13 CSV.dial CTV @10:03:22.15
CTV.dial CSV @10:03:22.35 ARV.dial :carrier @10:03:22.64
CBV.dial :waste2b @10:03:22.68 AS.seek 0.500ml @10:03:23.39
CS.seek 0.000ml @10:03:39.85 Disinfecting external Intake with kill @10:03:39.95 CSV.dial CSR @10:03:40.36 CSR.select :kill @10:03:40.46 CS.seek 8.000ml @10:03:48.58 AFV.select :carrierSolution @10:03:48.69 delay 2 seconds @10:03:50.70 AS.reconfigure ASconfig @10:03:50.73 AS.seek 1.000ml @10:03:59.35
delay 5 seconds @10:04:04.36 CSR.select :air @10:04:04.45 CS.seek 10.000ml @10:04:09.91 CSV.dial CBV @10:04:09.93
CBV.dial CSV @10:04:10.95 CTV.dial :intake @10:04:11.67 Intake.select :open @10:04:11.76 CS.seek 2.000ml @10:04:20.17 delay 1 second @10:04:21.18 AFV.select :holdingCoil @10:04:21.28 delay 1 second @10:04:22.29 ATV.dial :waste @10:04:23.56 AS.reconfigure ASnorm @10:04:23.59 AS.seek empty @10:04:30.84
Intake.select :close @10:04:30.93 CTV.dial :air @10:04:31.64 CS.reconfigure CSconfig @10:04:31.68 CS.seek 0.000ml @10:04:37.56 CSV.dial CSR @10:04:38.61 CSR.select :flush @10:04:38.71 CS.seek 5.000ml @10:04:52.25 delay 5 seconds @10:04:57.26 CSR.select :air @10:04:57.35 CS.seek 10.000ml @10:05:10.75 CSV.dial CBV @10:05:11.81 CS.seek 0.000ml @10:05:15.09 AFV.select :carrierSolution @10:05:15.19 delay 2 seconds @10:05:17.20 AS.reconfigure ASconfig @10:05:17.23 AS.seek 0.500ml @10:05:37.94
Garaging FlushPuck in Collection Clamp @10:05:38.00 Elbow.reconfigure TightElbow @10:05:38.03 Elbow.seek CC @10:05:38.12 Elbow.reconfigure LooseElbow @10:05:38.21 Forearm.seek CC @10:05:39.13 CC.seek sealed @10:05:40.06 Hand.grip @10:05:40.81 CC.seek open @10:05:44.13 Forearm.seek retract @10:05:45.06 Elbow.reconfigure TightElbow @10:05:45.10 Elbow.seek garage @10:05:46.73 delay 1 second @10:05:47.74 AFV.select :holdingCoil @10:05:47.83 Building Slug @10:05:47.84 ATV.dial :ARV @10:05:47.93
Elbow.reconfigure LooseElbow @10:05:48.02 Forearm.reconfigure TightForearm @10:05:48.05 Forearm.seek garage @10:05:49.10 delay 2 seconds @10:05:49.20
Hand.release @10:05:49.97 Forearm.reconfigure LooseForearm @10:05:50.00 Forearm.seek retract @10:05:51.10 ARV.dial :air1 @10:05:51.44 delay 2 seconds @10:05:53.50 AS.reconfigure ASslow2 @10:05:53.53 AS.seek 0.506ml @10:05:59.87 delay 2 seconds @10:06:01.88 ARV.dial :MM2 @10:06:03.11 delay 2 seconds @10:06:05.12 Adding MM2 @10:06:05.18 AS.seek 0.524ml @10:06:23.80 delay 25 seconds @10:06:48.81 ARV.dial :PP4 @10:06:49.23 Adding PP4 @10:06:49.24 delay 2 seconds @10:06:51.30 AS.seek 0.530ml @10:06:57.58 delay 10 seconds @10:07:07.59 ARV.dial :air3 @10:07:07.92 adding ARV air @10:07:07.93 delay 2 seconds @10:07:09.99 AS.seek 0.536ml @10:07:16.34 delay 5 seconds @10:07:21.35 ARV.dial :carrier @10:07:22.39 delay 7 seconds @10:07:29.45 AS.seek 0.594ml @10:08:28.68 delay 5 seconds @10:08:33.69 ATV.dial :ASV @10:08:34.01 delay 2 seconds @10:08:36.07 AS.seek 0.600ml @10:08:42.32 delay 4 seconds @10:08:46.33 ATV.dial ARV @10:08:46.65 delay 2 seconds @10:08:48.66 ARV.dial :carrier @10:08:48.67 delay 2 seconds @10:08:50.73 AS.seek 0.650ml @10:09:41.90 delay 4 seconds @10:09:45.91 ATV.dial PCR @10:09:46.41 ASV.dial :waste1 @10:09:47.09 ARV.dial :air1 @10:09:47.44 Positioning Slug by 0.324ml @10:09:47.49 AS.seek 0.326ml @10:15:18.11 delay 5 seconds @10:15:23.12 ATV.dial ARV @10:15:23.64 ASV.dial :PCRlock @10:15:23.97 delay 5 seconds @10:15:28.98 Running HF183em ProfileEM60C2 thermocycling @10:15:28.99 PCR.program ProfileEM60C2 @10:15:29.10 ProfileEM60C2 should complete in < 2:11:38 @10:15:29.11 PCR.run 2:49:32.5 @12:11:08.08 PCR.off @12:11:09.23 PCR for HF183em completed -- closed /var/log/esp/HF183em14mar1210h194ml.pcr @12:11:09.28 Cleaning PCR @12:11:09.29 AFV.select :carrierSolution @12:11:09.38 delay 2 seconds @12:11:11.39 AS.reconfigure ASconfig @12:11:11.42 AS.seek 0.500ml @12:11:21.88 delay 1 second @12:11:22.89 AFV.select :holdingCoil @12:11:22.98 ASV.dial :waste1 @12:11:23.73 ATV.dial :air3 @12:11:24.68 AS.reconfigure ASslow1 @12:11:24.71 AS.seek 0.510ml @12:11:27.01 delay 2 seconds @12:11:29.02 ATV.dial :bleach @12:11:29.51 AS.seek 0.810ml @12:12:30.93 delay 3 seconds @12:12:33.94 ATV.dial :air3 @12:12:34.27 delay 3 seconds @12:12:37.33 AS.seek 0.835ml @12:12:42.66 delay 3 seconds @12:12:45.67 ATV.dial :bleach @12:12:46.06 AS.seek 0.820ml @12:12:49.40 delay 3 seconds @12:12:52.41 ARV.dial :waste @12:12:52.91 delay 3 seconds @12:12:55.92 ATV.dial :ARV @12:12:56.64 AS.reconfigure ASconfig @12:12:56.68 AS.seek 0.470ml @12:13:17.36 AFV.select :carrierSolution @12:13:17.45 delay 2 seconds @12:13:19.46 AS.seek 1.000ml @12:13:50.67 delay 1 second @12:13:51.68 AFV.select :holdingCoil @12:13:51.77 delay 1 second @12:13:52.78 AS.reconfigure ASfast @12:13:52.81 AS.seek 0.000ml @12:14:20.53 AFV.select :carrierSolution @12:14:20.63 delay 2 seconds @12:14:22.64 AS.reconfigure ASconfig @12:14:22.67 AS.seek 1.000ml @12:15:21.28 delay 1 second @12:15:22.29 AFV.select :holdingCoil @12:15:22.38 delay 1 second @12:15:23.39 AS.reconfigure ASfast @12:15:23.42 AS.seek 0.000ml @12:15:51.14 AFV.select :carrierSolution @12:15:51.24 delay 2 seconds @12:15:53.25 AS.reconfigure ASconfig @12:15:53.28 AS.seek 1.000ml @12:16:51.84 delay 1 second @12:16:52.85 AFV.select :holdingCoil @12:16:52.94 delay 1 second @12:16:53.95 AS.reconfigure ASfast @12:16:53.99 AS.seek 0.000ml @12:17:21.72 ARV.dial between carrier and air1 @12:17:22.13 ATV.dial :air3 @12:17:23.14 AS.reconfigure ASslow1 @12:17:23.18 AS.seek 0.010ml @12:17:25.51 delay 2 seconds @12:17:27.52 ATV.dial :bleach @12:17:28.01 AS.seek 0.810ml @12:20:11.38 delay 3 seconds @12:20:14.39 ATV.dial :air3 @12:20:14.73 delay 3 seconds @12:20:17.79 AS.seek 0.835ml @12:20:23.13 delay 3 seconds @12:20:26.14 ATV.dial :bleach @12:20:26.53 AS.seek 0.820ml @12:20:29.84 delay 3 seconds @12:20:32.85 ATV.dial PCR @12:20:33.17 AS.reconfigure ASconfig @12:20:33.20 AS.seek empty @12:21:21.33 PCR.program :bleachProfile @12:21:21.39 PCR program should complete in < 2:45 @12:21:21.40 PCR.run 10:00 @12:24:19.59 PCR.off @12:24:19.62 AFV.select :carrierSolution @12:24:19.71 delay 2 seconds @12:24:21.72 AS.seek 1.000ml @12:25:20.37 delay 1 second @12:25:21.38 AFV.select :holdingCoil @12:25:21.47 delay 1 second @12:25:22.48 AS.reconfigure ASfast @12:25:22.51 AS.seek 0.000ml @12:25:50.22 delay 3 seconds @12:25:53.23 AFV.select :carrierSolution @12:25:53.32 delay 2 seconds @12:25:55.33 AS.reconfigure ASconfig @12:25:55.36 AS.seek 1.000ml @12:26:53.94 delay 1 second @12:26:54.95 AFV.select :holdingCoil @12:26:55.04 delay 1 second @12:26:56.05 AS.reconfigure ASfast @12:26:56.08 AS.seek 0.000ml @12:27:23.81 delay 3 seconds @12:27:26.82 AFV.select :carrierSolution @12:27:26.91 delay 2 seconds @12:27:28.92 AS.reconfigure ASconfig @12:27:28.95 AS.seek 1.000ml @12:28:27.56 delay 1 second @12:28:28.57 AFV.select :holdingCoil @12:28:28.66 delay 1 second @12:28:29.67 AS.reconfigure ASfast @12:28:29.70 AS.seek 0.000ml @12:28:57.42 delay 3 seconds @12:29:00.43 AFV.select :carrierSolution @12:29:00.52 delay 2 seconds @12:29:02.53 AS.reconfigure ASconfig @12:29:02.56 AS.seek 1.000ml @12:30:01.14 delay 1 second @12:30:02.15 AFV.select :holdingCoil @12:30:02.24 delay 1 second @12:30:03.25 AS.reconfigure ASfast @12:30:03.28 AS.seek 0.000ml @12:30:31.01 delay 3 seconds @12:30:34.02 AFV.select :carrierSolution @12:30:34.11 delay 2 seconds @12:30:36.12 AS.reconfigure ASconfig @12:30:36.15 AS.seek 1.000ml @12:31:34.74 delay 1 second @12:31:35.75 AFV.select :holdingCoil @12:31:35.84 delay 1 second @12:31:36.85 AS.reconfigure ASnorm @12:31:36.88 AS.seek 0.000ml @12:32:28.35 Setting up PCR for ENTap -- writing /var/log/esp/ENTap14mar1212h194ml.pcr @12:32:28.36 Building PCR Slug from ASV with MM1 mix, PP1 primer @12:32:28.37 Priming with carrier @12:32:28.38 ATV.dial ARV @12:32:28.98 ARV.dial :carrier @12:32:29.88 AS.seek 0.500ml @12:32:55.79 AFV.select :carrierSolution @12:32:55.88 delay 2 seconds @12:32:57.89 AS.reconfigure ASconfig @12:32:57.93 AS.seek 1.000ml @12:33:27.37 delay 1 second @12:33:28.38 AFV.select :holdingCoil @12:33:28.47 delay 1 second @12:33:29.48 ATV.dial :waste @12:33:30.74 AS.reconfigure ASnorm @12:33:30.77 AS.seek empty @12:34:22.25 AFV.select :carrierSolution @12:34:22.35 delay 2 seconds @12:34:24.36 AS.reconfigure ASconfig @12:34:24.46 AS.seek 0.500ml @12:34:53.93 delay 1 second @12:34:54.94 AFV.select :holdingCoil @12:34:55.03 Building Slug @12:34:55.04 ATV.dial :ARV @12:34:56.29 delay 2 seconds @12:34:58.30 ARV.dial :air1 @12:34:58.62 delay 2 seconds @12:35:00.68 AS.reconfigure ASslow2 @12:35:00.71 AS.seek 0.506ml @12:35:07.20 delay 2 seconds @12:35:09.21 ARV.dial :MM1 @12:35:09.72 delay 2 seconds @12:35:11.73 Adding MM1 @12:35:11.78 AS.seek 0.524ml @12:35:30.36 delay 25 seconds @12:35:55.37 ARV.dial :PP1 @12:35:55.70 Adding PP1 @12:35:55.71 delay 2 seconds @12:35:57.77 AS.seek 0.530ml @12:36:04.01 delay 10 seconds @12:36:14.02 ARV.dial :air1 @12:36:14.34 adding ARV air @12:36:14.35 delay 2 seconds @12:36:16.41 AS.seek 0.536ml @12:36:22.92 delay 5 seconds @12:36:27.93 ARV.dial :carrier @12:36:28.24 delay 7 seconds @12:36:35.30 AS.seek 0.594ml @12:37:34.87 delay 5 seconds @12:37:39.88 ATV.dial :ASV @12:37:40.21 delay 2 seconds @12:37:42.27 AS.seek 0.600ml @12:37:48.54 delay 4 seconds @12:37:52.55 ATV.dial ARV @12:37:52.88 delay 2 seconds @12:37:54.89 ARV.dial :carrier @12:37:54.90 delay 2 seconds @12:37:56.95 AS.seek 0.650ml @12:38:48.11 delay 4 seconds @12:38:52.12 ATV.dial PCR @12:38:52.61 ASV.dial :waste1 @12:38:52.62 ARV.dial :air1 @12:38:52.96 Positioning Slug by 0.324ml @12:38:53.02 AS.seek 0.326ml @12:44:23.71 delay 5 seconds @12:44:28.72 ATV.dial ARV @12:44:29.23 ASV.dial :PCRlock @12:44:29.65 delay 5 seconds @12:44:34.66 Running ENTap Profile60C thermocycling @12:44:34.67 PCR.program Profile60C @12:44:34.77 Profile60C should complete in < 1:39:08 @12:44:34.78 PCR.run 2:08:55 @14:07:26.43 PCR.off @14:07:26.95 PCR for ENTap completed -- closed /var/log/esp/ENTap14mar1212h194ml.pcr @14:07:27.00 Cleaning PCR @14:07:27.01 AFV.select :carrierSolution @14:07:27.10 delay 2 seconds @14:07:29.11 AS.reconfigure ASconfig @14:07:29.14 AS.seek 0.500ml @14:07:39.55 delay 1 second @14:07:40.56 AFV.select :holdingCoil @14:07:40.65 ASV.dial :waste1 @14:07:41.11 ATV.dial :air3 @14:07:42.05 AS.reconfigure ASslow1 @14:07:42.09 AS.seek 0.510ml @14:07:44.40 delay 2 seconds @14:07:46.41 ATV.dial :bleach @14:07:46.79 AS.seek 0.810ml @14:08:48.21 delay 3 seconds @14:08:51.22 ATV.dial :air3 @14:08:51.64 delay 3 seconds @14:08:54.70 AS.seek 0.835ml @14:09:00.03 delay 3 seconds @14:09:03.04 ATV.dial :bleach @14:09:03.41 AS.seek 0.820ml @14:09:06.74 delay 3 seconds @14:09:09.75 ARV.dial :waste @14:09:10.36 delay 3 seconds @14:09:13.37 ATV.dial :ARV @14:09:14.18 AS.reconfigure ASconfig @14:09:14.22 AS.seek 0.470ml @14:09:34.92 AFV.select :carrierSolution @14:09:35.01 delay 2 seconds @14:09:37.02 AS.seek 1.000ml @14:10:08.19 delay 1 second @14:10:09.20 AFV.select :holdingCoil @14:10:09.29 delay 1 second @14:10:10.30 AS.reconfigure ASfast @14:10:10.33 AS.seek 0.000ml @14:10:38.06 AFV.select :carrierSolution @14:10:38.15 delay 2 seconds @14:10:40.16 AS.reconfigure ASconfig @14:10:40.19 AS.seek 1.000ml @14:11:38.76 delay 1 second @14:11:39.77 AFV.select :holdingCoil @14:11:39.86 delay 1 second @14:11:40.87 AS.reconfigure ASfast @14:11:40.90 AS.seek 0.000ml @14:12:08.65 AFV.select :carrierSolution @14:12:08.75 delay 2 seconds @14:12:10.76 AS.reconfigure ASconfig @14:12:10.79 AS.seek 1.000ml @14:13:09.40 delay 1 second @14:13:10.41 AFV.select :holdingCoil @14:13:10.50 delay 1 second @14:13:11.51 AS.reconfigure ASfast @14:13:11.54 AS.seek 0.000ml @14:13:39.24 ARV.dial between carrier and air1 @14:13:39.74 ATV.dial :air3 @14:13:40.67 AS.reconfigure ASslow1 @14:13:40.71 AS.seek 0.010ml @14:13:43.04 delay 2 seconds @14:13:45.05 ATV.dial :bleach @14:13:45.45 AS.seek 0.810ml @14:16:28.84 delay 3 seconds @14:16:31.85 ATV.dial :air3 @14:16:32.19 delay 3 seconds @14:16:35.25 AS.seek 0.835ml @14:16:40.59 delay 3 seconds @14:16:43.60 ATV.dial :bleach @14:16:44.08 AS.seek 0.820ml @14:16:47.42 delay 3 seconds @14:16:50.43 ATV.dial PCR @14:16:50.76 AS.reconfigure ASconfig @14:16:50.80 AS.seek empty @14:17:38.95 PCR.program :bleachProfile @14:17:39.00 PCR program should complete in < 2:45 @14:17:39.01 PCR.run 10:00 @14:20:37.22 PCR.off @14:20:37.25 AFV.select :carrierSolution @14:20:37.34 delay 2 seconds @14:20:39.35 AS.seek 1.000ml @14:21:38.00 delay 1 second @14:21:39.01 AFV.select :holdingCoil @14:21:39.10 delay 1 second @14:21:40.11 AS.reconfigure ASfast @14:21:40.14 AS.seek 0.000ml @14:22:07.86 delay 3 seconds @14:22:10.87 AFV.select :carrierSolution @14:22:10.96 delay 2 seconds @14:22:12.97 AS.reconfigure ASconfig @14:22:13.00 AS.seek 1.000ml @14:23:11.58 delay 1 second @14:23:12.59 AFV.select :holdingCoil @14:23:12.68 delay 1 second @14:23:13.69 AS.reconfigure ASfast @14:23:13.72 AS.seek 0.000ml @14:23:41.44 delay 3 seconds @14:23:44.45 AFV.select :carrierSolution @14:23:44.54 delay 2 seconds @14:23:46.55 AS.reconfigure ASconfig @14:23:46.58 AS.seek 1.000ml @14:24:45.18 delay 1 second @14:24:46.19 AFV.select :holdingCoil @14:24:46.28 delay 1 second @14:24:47.29 AS.reconfigure ASfast @14:24:47.32 AS.seek 0.000ml @14:25:15.06 delay 3 seconds @14:25:18.07 AFV.select :carrierSolution @14:25:18.16 delay 2 seconds @14:25:20.17 AS.reconfigure ASconfig @14:25:20.20 AS.seek 1.000ml @14:26:18.79 delay 1 second @14:26:19.80 AFV.select :holdingCoil @14:26:20.48 delay 1 second @14:26:21.49 AS.reconfigure ASfast @14:26:21.52 AS.seek 0.000ml @14:26:49.26 delay 3 seconds @14:26:52.27 AFV.select :carrierSolution @14:26:52.36 delay 2 seconds @14:26:54.37 AS.reconfigure ASconfig @14:26:54.40 AS.seek 1.000ml @14:27:52.99 delay 1 second @14:27:54.00 AFV.select :holdingCoil @14:27:54.09 delay 1 second @14:27:55.10 AS.reconfigure ASnorm @14:27:55.13 AS.seek 0.000ml @14:28:46.60 Setting up PCR for ENTem -- writing /var/log/esp/ENTem14mar1214h194ml.pcr @14:28:46.61 Building PCR Slug from ASV with MM2 mix, PP3 primer @14:28:46.62 Priming with carrier @14:28:46.63 ATV.dial ARV @14:28:47.15 ARV.dial :carrier @14:28:47.43 AS.seek 0.500ml @14:29:13.33 AFV.select :carrierSolution @14:29:13.43 delay 2 seconds @14:29:15.44 AS.reconfigure ASconfig @14:29:15.48 AS.seek 1.000ml @14:29:44.92 delay 1 second @14:29:45.93 AFV.select :holdingCoil @14:29:46.02 delay 1 second @14:29:47.03 ATV.dial :waste @14:29:48.40 AS.reconfigure ASnorm @14:29:48.43 AS.seek empty @14:30:39.92 AFV.select :carrierSolution @14:30:40.02 delay 2 seconds @14:30:42.03 AS.reconfigure ASconfig @14:30:42.06 AS.seek 0.500ml @14:31:11.55 delay 1 second @14:31:12.56 AFV.select :holdingCoil @14:31:12.65 Building Slug @14:31:12.66 ATV.dial :ARV @14:31:13.89 delay 2 seconds @14:31:15.90 ARV.dial :air1 @14:31:16.23 delay 2 seconds @14:31:18.29 AS.reconfigure ASslow2 @14:31:18.32 AS.seek 0.506ml @14:31:24.73 delay 2 seconds @14:31:26.74 ARV.dial :MM2 @14:31:27.95 delay 2 seconds @14:31:29.96 Adding MM2 @14:31:30.02 AS.seek 0.524ml @14:31:48.48 delay 25 seconds @14:32:13.49 ARV.dial :PP3 @14:32:13.82 Adding PP3 @14:32:13.83 delay 2 seconds @14:32:15.88 AS.seek 0.530ml @14:32:22.26 delay 10 seconds @14:32:32.27 ARV.dial :air2 @14:32:32.70 adding ARV air @14:32:32.71 delay 2 seconds @14:32:34.76 AS.seek 0.536ml @14:32:41.16 delay 5 seconds @14:32:46.17 ARV.dial :carrier @14:32:47.19 delay 7 seconds @14:32:54.41 AS.seek 0.594ml @14:33:54.07 delay 5 seconds @14:33:59.08 ATV.dial :ASV @14:33:59.41 delay 2 seconds @14:34:01.47 AS.seek 0.600ml @14:34:07.88 delay 4 seconds @14:34:11.89 ATV.dial ARV @14:34:12.33 delay 2 seconds @14:34:14.34 ARV.dial :carrier @14:34:14.35 delay 2 seconds @14:34:16.40 AS.seek 0.650ml @14:35:07.58 delay 4 seconds @14:35:11.59 ATV.dial PCR @14:35:12.11 ASV.dial :waste1 @14:35:12.12 ARV.dial :air1 @14:35:12.47 Positioning Slug by 0.324ml @14:35:12.52 AS.seek 0.326ml @14:40:43.02 delay 5 seconds @14:40:48.03 ATV.dial ARV @14:40:48.54 ASV.dial :PCRlock @14:40:49.02 delay 5 seconds @14:40:54.03 Running ENTem ProfileEM60C thermocycling @14:40:54.05 PCR.program ProfileEM60C @14:40:54.16 ProfileEM60C should complete in < 2:11:38 @14:40:54.17 PCR.run 2:49:32.5 @16:36:51.51 PCR.off @16:36:52.05 PCR for ENTem completed -- closed /var/log/esp/ENTem14mar1214h194ml.pcr @16:36:52.10 Cleaning PCR @16:36:52.11 AFV.select :carrierSolution @16:36:52.20 delay 2 seconds @16:36:54.21 AS.reconfigure ASconfig @16:36:54.25 AS.seek 0.500ml @16:37:04.69 delay 1 second @16:37:05.70 AFV.select :holdingCoil @16:37:05.79 ASV.dial :waste1 @16:37:06.27 ATV.dial :air3 @16:37:07.21 AS.reconfigure ASslow1 @16:37:07.24 AS.seek 0.510ml @16:37:09.54 delay 2 seconds @16:37:11.55 ATV.dial :bleach @16:37:12.04 AS.seek 0.810ml @16:38:13.45 delay 3 seconds @16:38:16.46 ATV.dial :air3 @16:38:16.78 delay 3 seconds @16:38:19.84 AS.seek 0.835ml @16:38:25.17 delay 3 seconds @16:38:28.18 ATV.dial :bleach @16:38:28.57 AS.seek 0.820ml @16:38:31.88 delay 3 seconds @16:38:34.89 ARV.dial :waste @16:38:35.49 delay 3 seconds @16:38:38.50 ATV.dial :ARV @16:38:39.24 AS.reconfigure ASconfig @16:38:39.27 AS.seek 0.470ml @16:38:59.94 AFV.select :carrierSolution @16:39:00.03 delay 2 seconds @16:39:02.04 AS.seek 1.000ml @16:39:33.25 delay 1 second @16:39:34.26 AFV.select :holdingCoil @16:39:34.35 delay 1 second @16:39:35.36 AS.reconfigure ASfast @16:39:35.39 AS.seek 0.000ml @16:40:03.12 AFV.select :carrierSolution @16:40:03.21 delay 2 seconds @16:40:05.22 AS.reconfigure ASconfig @16:40:05.25 AS.seek 1.000ml @16:41:03.82 delay 1 second @16:41:04.83 AFV.select :holdingCoil @16:41:04.92 delay 1 second @16:41:05.93 AS.reconfigure ASfast @16:41:05.96 AS.seek 0.000ml @16:41:33.70 AFV.select :carrierSolution @16:41:33.80 delay 2 seconds @16:41:35.81 AS.reconfigure ASconfig @16:41:35.85 AS.seek 1.000ml @16:42:34.45 delay 1 second @16:42:35.46 AFV.select :holdingCoil @16:42:35.55 delay 1 second @16:42:36.56 AS.reconfigure ASfast @16:42:36.59 AS.seek 0.000ml @16:43:04.32 ARV.dial between carrier and air1 @16:43:04.73 ATV.dial :air3 @16:43:05.67 AS.reconfigure ASslow1 @16:43:05.71 AS.seek 0.010ml @16:43:08.04 delay 2 seconds @16:43:10.05 ATV.dial :bleach @16:43:10.45 AS.seek 0.810ml @16:45:53.83 delay 3 seconds @16:45:56.84 ATV.dial :air3 @16:45:57.17 delay 3 seconds @16:46:00.23 AS.seek 0.835ml @16:46:05.55 delay 3 seconds @16:46:08.56 ATV.dial :bleach @16:46:08.96 AS.seek 0.820ml @16:46:12.25 delay 3 seconds @16:46:15.26 ATV.dial PCR @16:46:15.56 AS.reconfigure ASconfig @16:46:15.60 AS.seek empty @16:47:03.72 PCR.program :bleachProfile @16:47:03.77 PCR program should complete in < 2:45 @16:47:03.78 PCR.run 10:00 @16:50:01.98 PCR.off @16:50:02.01 AFV.select :carrierSolution @16:50:02.10 delay 2 seconds @16:50:04.11 AS.seek 1.000ml @16:51:02.74 delay 1 second @16:51:03.75 AFV.select :holdingCoil @16:51:03.84 delay 1 second @16:51:04.85 AS.reconfigure ASfast @16:51:04.88 AS.seek 0.000ml @16:51:32.61 delay 3 seconds @16:51:35.62 AFV.select :carrierSolution @16:51:35.71 delay 2 seconds @16:51:38.34 AS.reconfigure ASconfig @16:51:38.37 AS.seek 1.000ml @16:52:37.00 delay 1 second @16:52:38.01 AFV.select :holdingCoil @16:52:38.10 delay 1 second @16:52:39.11 AS.reconfigure ASfast @16:52:39.15 AS.seek 0.000ml @16:53:06.86 delay 3 seconds @16:53:09.87 AFV.select :carrierSolution @16:53:09.96 delay 2 seconds @16:53:11.97 AS.reconfigure ASconfig @16:53:12.00 AS.seek 1.000ml @16:54:10.59 delay 1 second @16:54:11.60 AFV.select :holdingCoil @16:54:11.69 delay 1 second @16:54:12.70 AS.reconfigure ASfast @16:54:12.73 AS.seek 0.000ml @16:54:40.46 delay 3 seconds @16:54:43.47 AFV.select :carrierSolution @16:54:43.56 delay 2 seconds @16:54:45.57 AS.reconfigure ASconfig @16:54:45.60 AS.seek 1.000ml @16:55:44.18 delay 1 second @16:55:45.19 AFV.select :holdingCoil @16:55:45.28 delay 1 second @16:55:46.29 AS.reconfigure ASfast @16:55:46.32 AS.seek 0.000ml @16:56:14.05 delay 3 seconds @16:56:17.06 AFV.select :carrierSolution @16:56:17.15 delay 2 seconds @16:56:19.16 AS.reconfigure ASconfig @16:56:19.19 AS.seek 1.000ml @16:57:17.80 delay 1 second @16:57:18.81 AFV.select :holdingCoil @16:57:18.90 delay 1 second @16:57:19.91 AS.reconfigure ASnorm @16:57:19.94 AS.seek 0.000ml @16:58:11.39 Setting up PCR for IPCap -- writing /var/log/esp/IPCap14mar1216h194ml.pcr @16:58:11.40 Building PCR Slug from ASV with MM1 mix, PP2 primer @16:58:11.41 Priming with carrier @16:58:11.42 ATV.dial ARV @16:58:12.00 ARV.dial :carrier @16:58:12.29 AS.seek 0.500ml @16:58:38.21 AFV.select :carrierSolution @16:58:38.31 delay 2 seconds @16:58:40.32 AS.reconfigure ASconfig @16:58:40.36 AS.seek 1.000ml @16:59:09.75 delay 1 second @16:59:10.76 AFV.select :holdingCoil @16:59:10.85 delay 1 second @16:59:11.86 ATV.dial :waste @16:59:13.12 AS.reconfigure ASnorm @16:59:13.15 AS.seek empty @17:00:04.63 AFV.select :carrierSolution @17:00:04.73 delay 2 seconds @17:00:06.74 AS.reconfigure ASconfig @17:00:06.77 AS.seek 0.500ml @17:00:36.25 delay 1 second @17:00:37.26 AFV.select :holdingCoil @17:00:37.35 Building Slug @17:00:37.36 ATV.dial :ARV @17:00:38.61 delay 2 seconds @17:00:40.62 ARV.dial :air1 @17:00:40.95 delay 2 seconds @17:00:43.01 AS.reconfigure ASslow2 @17:00:43.04 AS.seek 0.506ml @17:00:49.42 delay 2 seconds @17:00:51.43 ARV.dial :MM1 @17:00:51.92 delay 2 seconds @17:00:53.93 Adding MM1 @17:00:53.98 AS.seek 0.524ml @17:01:12.83 delay 25 seconds @17:01:37.84 ARV.dial :PP2 @17:01:38.17 Adding PP2 @17:01:38.18 delay 2 seconds @17:01:40.24 AS.seek 0.530ml @17:01:46.56 delay 10 seconds @17:01:56.57 ARV.dial :air2 @17:01:56.89 adding ARV air @17:01:56.90 delay 2 seconds @17:01:58.96 AS.seek 0.536ml @17:02:05.47 delay 5 seconds @17:02:10.48 ARV.dial :carrier @17:02:11.51 delay 7 seconds @17:02:18.57 AS.seek 0.594ml @17:03:18.06 delay 5 seconds @17:03:23.07 ATV.dial :ASV @17:03:23.40 delay 2 seconds @17:03:25.46 AS.seek 0.600ml @17:03:31.90 delay 4 seconds @17:03:35.91 ATV.dial ARV @17:03:36.24 delay 2 seconds @17:03:38.25 ARV.dial :carrier @17:03:38.26 delay 2 seconds @17:03:40.31 AS.seek 0.650ml @17:04:31.57 delay 4 seconds @17:04:35.58 ATV.dial PCR @17:04:36.09 ASV.dial :waste1 @17:04:36.10 ARV.dial :air1 @17:04:36.43 Positioning Slug by 0.324ml @17:04:36.49 AS.seek 0.326ml @17:10:06.93 delay 5 seconds @17:10:11.94 ATV.dial ARV @17:10:12.46 ASV.dial :PCRlock @17:10:12.94 delay 5 seconds @17:10:17.95 Running IPCap Profile59C thermocycling @17:10:17.96 PCR.program Profile59C @17:10:18.06 Profile59C should complete in < 1:39:08 @17:10:18.07 PCR.run 2:08:55 @18:33:55.18 PCR.off @18:33:55.72 PCR for IPCap completed -- closed /var/log/esp/IPCap14mar1216h194ml.pcr @18:33:55.77 Cleaning PCR @18:33:55.78 AFV.select :carrierSolution @18:33:55.87 delay 2 seconds @18:33:57.88 AS.reconfigure ASconfig @18:33:57.91 AS.seek 0.500ml @18:34:08.33 delay 1 second @18:34:09.34 AFV.select :holdingCoil @18:34:09.43 ASV.dial :waste1 @18:34:10.17 ATV.dial :air3 @18:34:11.11 AS.reconfigure ASslow1 @18:34:11.15 AS.seek 0.510ml @18:34:13.44 delay 2 seconds @18:34:15.45 ATV.dial :bleach @18:34:15.83 AS.seek 0.810ml @18:35:17.27 delay 3 seconds @18:35:20.28 ATV.dial :air3 @18:35:20.70 delay 3 seconds @18:35:23.76 AS.seek 0.835ml @18:35:29.13 delay 3 seconds @18:35:32.14 ATV.dial :bleach @18:35:32.53 AS.seek 0.820ml @18:35:35.84 delay 3 seconds @18:35:38.85 ARV.dial :waste @18:35:39.35 delay 3 seconds @18:35:42.35 ATV.dial :ARV @18:35:43.07 AS.reconfigure ASconfig @18:35:43.11 AS.seek 0.470ml @18:36:03.80 AFV.select :carrierSolution @18:36:03.89 delay 2 seconds @18:36:05.90 AS.seek 1.000ml @18:36:37.12 delay 1 second @18:36:38.13 AFV.select :holdingCoil @18:36:38.22 delay 1 second @18:36:39.23 AS.reconfigure ASfast @18:36:39.26 AS.seek 0.000ml @18:37:07.01 AFV.select :carrierSolution @18:37:07.10 delay 2 seconds @18:37:09.11 AS.reconfigure ASconfig @18:37:09.14 AS.seek 1.000ml @18:38:07.73 delay 1 second @18:38:08.74 AFV.select :holdingCoil @18:38:08.83 delay 1 second @18:38:09.84 AS.reconfigure ASfast @18:38:09.87 AS.seek 0.000ml @18:38:37.60 AFV.select :carrierSolution @18:38:37.70 delay 2 seconds @18:38:39.71 AS.reconfigure ASconfig @18:38:39.74 AS.seek 1.000ml @18:39:38.32 delay 1 second @18:39:39.33 AFV.select :holdingCoil @18:39:39.42 delay 1 second @18:39:40.43 AS.reconfigure ASfast @18:39:40.46 AS.seek 0.000ml @18:40:08.19 ARV.dial between carrier and air1 @18:40:08.60 ATV.dial :air3 @18:40:09.53 AS.reconfigure ASslow1 @18:40:09.57 AS.seek 0.010ml @18:40:11.90 delay 2 seconds @18:40:13.91 ATV.dial :bleach @18:40:14.31 AS.seek 0.810ml @18:42:57.70 delay 3 seconds @18:43:00.71 ATV.dial :air3 @18:43:01.05 delay 3 seconds @18:43:04.11 AS.seek 0.835ml @18:43:09.47 delay 3 seconds @18:43:12.48 ATV.dial :bleach @18:43:12.88 AS.seek 0.820ml @18:43:16.19 delay 3 seconds @18:43:19.20 ATV.dial PCR @18:43:19.52 AS.reconfigure ASconfig @18:43:19.56 AS.seek empty @18:44:07.69 PCR.program :bleachProfile @18:44:07.74 PCR program should complete in < 2:45 @18:44:07.75 PCR.run 10:00 @18:47:06.58 PCR.off @18:47:06.61 AFV.select :carrierSolution @18:47:06.70 delay 2 seconds @18:47:08.71 AS.seek 1.000ml @18:48:07.37 delay 1 second @18:48:08.38 AFV.select :holdingCoil @18:48:08.47 delay 1 second @18:48:09.48 AS.reconfigure ASfast @18:48:09.66 AS.seek 0.000ml @18:48:37.41 delay 3 seconds @18:48:40.42 AFV.select :carrierSolution @18:48:40.51 delay 2 seconds @18:48:42.52 AS.reconfigure ASconfig @18:48:42.55 AS.seek 1.000ml @18:49:41.14 delay 1 second @18:49:42.15 AFV.select :holdingCoil @18:49:42.24 delay 1 second @18:49:43.25 AS.reconfigure ASfast @18:49:43.28 AS.seek 0.000ml @18:50:11.01 delay 3 seconds @18:50:14.02 AFV.select :carrierSolution @18:50:14.11 delay 2 seconds @18:50:16.12 AS.reconfigure ASconfig @18:50:16.15 AS.seek 1.000ml @18:51:14.76 delay 1 second @18:51:15.77 AFV.select :holdingCoil @18:51:15.86 delay 1 second @18:51:16.87 AS.reconfigure ASfast @18:51:16.90 AS.seek 0.000ml @18:51:44.64 delay 3 seconds @18:51:47.65 AFV.select :carrierSolution @18:51:47.74 delay 2 seconds @18:51:49.75 AS.reconfigure ASconfig @18:51:49.78 AS.seek 1.000ml @18:52:48.37 delay 1 second @18:52:49.38 AFV.select :holdingCoil @18:52:49.47 delay 1 second @18:52:50.48 AS.reconfigure ASfast @18:52:50.51 AS.seek 0.000ml @18:53:18.21 delay 3 seconds @18:53:21.22 AFV.select :carrierSolution @18:53:21.31 delay 2 seconds @18:53:23.32 AS.reconfigure ASconfig @18:53:23.35 AS.seek 1.000ml @18:54:21.94 delay 1 second @18:54:22.95 AFV.select :holdingCoil @18:54:23.04 delay 1 second @18:54:24.05 AS.reconfigure ASnorm @18:54:24.08 AS.seek 0.000ml @18:55:15.55 Setting up PCR for IPCem -- writing /var/log/esp/IPCem14mar1218h194ml.pcr @18:55:15.56 Building PCR Slug from ASV with MM3 mix, PP5 primer @18:55:15.57 Priming with carrier @18:55:15.58 ATV.dial ARV @18:55:16.10 ARV.dial :carrier @18:55:16.38 AS.seek 0.500ml @18:55:42.25 AFV.select :carrierSolution @18:55:42.34 delay 2 seconds @18:55:44.35 AS.reconfigure ASconfig @18:55:44.39 AS.seek 1.000ml @18:56:13.83 delay 1 second @18:56:14.84 AFV.select :holdingCoil @18:56:14.94 delay 1 second @18:56:15.95 ATV.dial :waste @18:56:17.21 AS.reconfigure ASnorm @18:56:17.24 AS.seek empty @18:57:08.72 AFV.select :carrierSolution @18:57:08.82 delay 2 seconds @18:57:10.83 AS.reconfigure ASconfig @18:57:10.86 AS.seek 0.500ml @18:57:40.34 delay 1 second @18:57:41.35 AFV.select :holdingCoil @18:57:41.44 Building Slug @18:57:41.45 ATV.dial :ARV @18:57:42.78 delay 2 seconds @18:57:44.79 ARV.dial :air1 @18:57:45.12 delay 2 seconds @18:57:47.18 AS.reconfigure ASslow2 @18:57:47.21 AS.seek 0.506ml @18:57:53.73 delay 2 seconds @18:57:55.74 ARV.dial :MM3 @18:57:56.59 delay 2 seconds @18:57:58.60 Adding MM3 @18:57:58.65 AS.seek 0.524ml @18:58:17.26 delay 25 seconds @18:58:42.27 ARV.dial :PP5 @18:58:42.67 Adding PP5 @18:58:42.68 delay 2 seconds @18:58:44.74 AS.seek 0.530ml @18:58:51.00 delay 10 seconds @18:59:01.01 ARV.dial :air3 @18:59:01.43 adding ARV air @18:59:01.44 delay 2 seconds @18:59:03.50 AS.seek 0.536ml @18:59:09.82 delay 5 seconds @18:59:14.83 ARV.dial :carrier @18:59:15.84 delay 7 seconds @18:59:22.90 AS.seek 0.594ml @19:00:22.46 delay 5 seconds @19:00:27.47 ATV.dial :ASV @19:00:27.81 delay 2 seconds @19:00:29.87 AS.seek 0.600ml @19:00:36.37 delay 4 seconds @19:00:40.38 ATV.dial ARV @19:00:40.71 delay 2 seconds @19:00:42.72 ARV.dial :carrier @19:00:42.73 delay 2 seconds @19:00:44.78 AS.seek 0.650ml @19:01:36.26 delay 4 seconds @19:01:40.27 ATV.dial PCR @19:01:40.77 ASV.dial :waste1 @19:01:40.78 ARV.dial :air1 @19:01:41.12 Positioning Slug by 0.324ml @19:01:41.18 AS.seek 0.326ml @19:07:11.71 delay 5 seconds @19:07:16.72 ATV.dial ARV @19:07:17.24 ASV.dial :PCRlock @19:07:17.57 delay 5 seconds @19:07:22.58 Running IPCem ProfileEM59C thermocycling @19:07:22.59 PCR.program ProfileEM59C @19:07:22.70 ProfileEM59C should complete in < 2:11:38 @19:07:22.71 PCR.run 2:49:32.5 @21:02:53.02 PCR.off @21:02:53.56 PCR for IPCem completed -- closed /var/log/esp/IPCem14mar1218h194ml.pcr @21:02:53.57 Full MFB Decontamination Decon extraction column and decon AIV @21:02:53.59 SPE.hold 2:46:40,55.0C @21:02:53.60 ASV.dial :SPEdelivery @21:02:54.30 delay 20 seconds @21:03:14.31 AFV.select :carrierSolution @21:03:14.40 delay 2 seconds @21:03:16.41 AS.reconfigure ASconfig @21:03:16.44 AS.seek 0.500ml @21:03:26.85 delay 1 second @21:03:27.86 AFV.select :holdingCoil @21:03:27.95 ATV.dial :air1 @21:03:29.48 AS.reconfigure ASslow1 @21:03:29.51 AS.seek 0.520ml @21:03:33.86 ATV.dial :ASV @21:03:35.14 ASV.dial :SPErecovery @21:03:35.69 AS.seek 0.620ml @21:03:56.32 ATV.dial :waste @21:03:57.79 AS.reconfigure ASnorm @21:03:57.82 AS.seek empty @21:04:29.88 AFV.select :holdingCoil @21:04:29.89 ATV.dial :waste @21:04:29.90 AS.reconfigure ASconfig @21:04:29.93 AS.seek 0.000ml @21:04:30.22 AFV.select :carrierSolution @21:04:30.31 delay 2 seconds @21:04:32.32 AS.seek 0.500ml @21:05:01.75 delay 1 second @21:05:02.76 AFV.select :holdingCoil @21:05:02.85 ATV.dial :air3 @21:05:03.41 AS.reconfigure ASslow1 @21:05:03.45 AS.seek 0.520ml @21:05:07.81 ATV.dial :air3 @21:05:07.87 AS.seek 0.530ml @21:05:10.14 delay 2 seconds @21:05:12.15 ATV.dial :bleach @21:05:12.53 AS.seek 0.710ml @21:05:49.49 delay 3 seconds @21:05:52.50 ATV.dial :air3 @21:05:52.84 delay 3 seconds @21:05:55.90 AS.seek 0.735ml @21:06:01.26 delay 3 seconds @21:06:04.27 ATV.dial :bleach @21:06:04.76 AS.seek 0.720ml @21:06:08.10 delay 3 seconds @21:06:11.11 ATV.dial ASV @21:06:11.99 ASV.dial :SPErecovery @21:06:12.05 AS.seek 0.365ml @21:07:24.69 ATV.dial :waste @21:07:26.27 AS.reconfigure ASnorm @21:07:26.30 AS.seek empty @21:07:45.33 ATV.dial :air3 @21:07:46.58 AS.reconfigure ASslow1 @21:07:46.61 AS.seek 0.009ml @21:07:48.96 delay 2 seconds @21:07:50.97 ATV.dial :bleach @21:07:51.35 AS.seek 0.579ml @21:09:47.80 delay 3 seconds @21:09:50.81 ATV.dial :air3 @21:09:51.16 delay 3 seconds @21:09:54.22 AS.seek 0.604ml @21:09:59.60 delay 3 seconds @21:10:02.61 ATV.dial :bleach @21:10:03.10 AS.seek 0.589ml @21:10:06.41 delay 3 seconds @21:10:09.42 ATV.dial :ASV @21:10:10.42 ASV.dial :wasteR @21:10:11.18 AS.seek 0.000ml @21:12:11.72 delay 1:00 @21:13:11.73 ATV.dial ASV @21:13:11.74 AFV.select :carrierSolution @21:13:11.83 delay 2 seconds @21:13:13.84 AS.reconfigure ASconfig @21:13:13.87 AS.seek 1.000ml @21:14:12.42 delay 1 second @21:14:13.43 AFV.select :holdingCoil @21:14:13.52 delay 1 second @21:14:14.53 ASV.dial :wasteR @21:14:14.54 AS.reconfigure ASslow1 @21:14:14.57 AS.seek 0.000ml @21:17:38.86 delay 10 seconds @21:17:48.87 ATV.dial :air3 @21:17:49.97 AS.seek 0.010ml @21:17:52.33 delay 2 seconds @21:17:54.34 ATV.dial :bleach @21:17:54.72 AS.seek 0.580ml @21:19:51.23 delay 3 seconds @21:19:54.24 ATV.dial :air3 @21:19:54.72 delay 3 seconds @21:19:57.78 AS.seek 0.605ml @21:20:03.14 delay 3 seconds @21:20:06.15 ATV.dial :bleach @21:20:06.55 AS.seek 0.590ml @21:20:09.84 delay 3 seconds @21:20:12.85 ATV.dial ASV @21:20:13.72 ASV.dial :SPEdelivery @21:20:14.48 AS.seek 0.000ml @21:22:15.04 delay 1:00 @21:23:15.05 AFV.select :carrierSolution @21:23:15.14 delay 2 seconds @21:23:17.15 AS.reconfigure ASconfig @21:23:17.18 AS.seek 1.000ml @21:24:15.77 delay 1 second @21:24:16.78 AFV.select :holdingCoil @21:24:16.87 delay 1 second @21:24:17.88 ATV.dial ASV @21:24:17.89 ASV.dial :SPEdelivery @21:24:17.90 AS.reconfigure ASslow1 @21:24:17.93 AS.seek 0.000ml @21:27:42.17 delay 10 seconds @21:27:52.18 ATV.dial :air2 @21:27:52.91 AS.reconfigure ASslow0 @21:27:52.94 AS.seek 0.800ml @21:29:14.85 delay 3 seconds @21:29:17.86 ATV.dial :ASV @21:29:18.57 ASV.dial :SPEdelivery @21:29:18.59 AS.reconfigure ASslow1 @21:29:18.62 AS.seek 0.030ml @21:31:55.89 delay 20 seconds @21:32:15.90 ATV.dial :waste,via => air3 @21:32:17.35 AS.reconfigure ASconfig @21:32:17.38 AS.seek empty @21:32:19.47 SPE.coast @21:32:19.55 Removing Bleach from Mixing Coil AFV.select :carrierSolution @21:32:19.65 delay 2 seconds @21:32:21.66 AS.seek 0.500ml @21:32:51.14 delay 1 second @21:32:52.15 AFV.select :holdingCoil @21:32:52.24 ATV.dial :ASV @21:32:53.69 ASV.dial :SPErecovery @21:32:54.49 AS.reconfigure ASslow1 @21:32:54.53 AS.seek 0.730ml @21:33:41.69 ASV.dial :wasteD @21:33:42.35 AS.reconfigure ASnorm @21:33:42.38 AS.seek 0.000ml @21:34:20.00 AFV.select :carrierSolution @21:34:20.09 delay 2 seconds @21:34:22.10 AS.reconfigure ASconfig @21:34:22.13 AS.seek 0.500ml @21:34:51.57 delay 1 second @21:34:52.58 AFV.select :holdingCoil @21:34:52.67 ATV.dial ASV @21:34:52.68 ASV.dial :SPErecovery @21:34:53.38 AS.reconfigure ASslow0 @21:34:53.42 AS.seek 0.300ml @21:35:14.09 ATV.dial :air2 @21:35:14.94 AS.reconfigure ASslow1 @21:35:14.98 AS.seek 0.320ml @21:35:19.34 ATV.dial ASV @21:35:20.08 AS.reconfigure ASslow0 @21:35:20.12 AS.seek 0.570ml @21:35:45.90 ASV.dial :wasteD @21:35:46.57 AS.reconfigure ASnorm @21:35:46.60 AS.seek 0.000ml @21:36:16.06 AFV.select :carrierSolution @21:36:16.15 delay 2 seconds @21:36:18.16 AS.reconfigure ASconfig @21:36:18.19 AS.seek 0.500ml @21:36:47.65 delay 1 second @21:36:48.66 AFV.select :holdingCoil @21:36:48.75 ATV.dial ASV @21:36:48.76 ASV.dial :SPErecovery @21:36:49.46 AS.reconfigure ASslow0 @21:36:49.49 AS.seek 0.300ml @21:37:10.16 ATV.dial :air2 @21:37:11.02 AS.reconfigure ASslow1 @21:37:11.06 AS.seek 0.320ml @21:37:15.41 ATV.dial ASV @21:37:16.25 AS.reconfigure ASslow0 @21:37:16.29 AS.seek 0.570ml @21:37:42.10 ASV.dial :wasteD @21:37:42.77 AS.reconfigure ASnorm @21:37:42.80 AS.seek 0.000ml @21:38:12.24 ATV.dial :air2 @21:38:13.64 AS.reconfigure ASslow0 @21:38:13.67 AS.seek 0.300ml @21:38:44.54 ATV.dial :ASV @21:38:45.33 ASV.dial :wasteD @21:38:45.35 AS.reconfigure ASconfig @21:38:45.38 AS.seek 0.050ml @21:39:00.23 ATV.dial :waste @21:39:01.68 AS.seek 0.000ml @21:39:04.90 ASV.dial :SPEdelivery @21:39:05.92 deconLysateINT @21:39:05.93 ATV.dial :air3 @21:39:06.49 AS.reconfigure ASslow1 @21:39:06.53 AS.seek 0.010ml @21:39:08.87 delay 2 seconds @21:39:10.88 ATV.dial :bleach @21:39:11.25 AS.seek 0.960ml @21:42:25.26 delay 3 seconds @21:42:28.27 ATV.dial :air3 @21:42:28.62 delay 3 seconds @21:42:31.68 AS.seek 0.985ml @21:42:36.99 delay 3 seconds @21:42:40.00 ATV.dial :bleach @21:42:40.39 AS.seek 0.970ml @21:42:43.68 delay 3 seconds @21:42:46.69 ATV.dial :AIV @21:42:47.76 AIV.dial :coilToMFB @21:42:47.77 AWV.select :waste @21:42:47.78 AFV.select :carrierSolution @21:42:47.87 delay 2 seconds @21:42:49.88 AS.reconfigure ASconfig @21:42:49.91 AS.seek 1.000ml @21:42:51.98 delay 1 second @21:42:52.99 AFV.select :holdingCoil @21:42:53.08 delay 1 second @21:42:54.09 AS.reconfigure ASslow0 @21:42:54.12 AS.seek empty @21:44:36.52 AFV.select :carrierSolution @21:44:36.61 delay 2 seconds @21:44:38.62 AS.reconfigure ASconfig @21:44:38.65 AS.seek 1.000ml @21:45:37.31 delay 1 second @21:45:38.32 AFV.select :holdingCoil @21:45:38.41 delay 1 second @21:45:39.42 AS.reconfigure ASslow0 @21:45:39.46 AS.seek empty @21:47:21.87 AFV.select :carrierSolution @21:47:21.96 delay 2 seconds @21:47:23.97 AS.reconfigure ASconfig @21:47:24.00 AS.seek 1.000ml @21:48:22.65 delay 1 second @21:48:23.66 AFV.select :holdingCoil @21:48:23.75 delay 1 second @21:48:24.76 AS.reconfigure ASslow0 @21:48:24.79 AS.seek empty @21:50:07.18 ATV.dial :air2 @21:50:07.85 AS.reconfigure ASslow1 @21:50:07.89 AS.seek 0.009ml @21:50:10.24 AS.reconfigure ASslow0 @21:50:10.28 AS.seek 1.000ml @21:51:51.61 ATV.dial AIV @21:51:52.14 AS.seek 0.020ml @21:53:32.46 ATV.dial :waste @21:53:33.82 AS.seek 0.000ml @21:53:36.11 AWV.select :air @21:53:36.25 Cleaning PCR @21:53:36.26 AFV.select :carrierSolution @21:53:36.35 delay 2 seconds @21:53:38.36 AS.reconfigure ASconfig @21:53:38.39 AS.seek 0.500ml @21:54:07.83 delay 1 second @21:54:08.84 AFV.select :holdingCoil @21:54:08.93 ASV.dial :waste1 @21:54:09.47 ATV.dial :air3 @21:54:10.04 AS.reconfigure ASslow1 @21:54:10.07 AS.seek 0.510ml @21:54:12.39 delay 2 seconds @21:54:14.40 ATV.dial :bleach @21:54:14.77 AS.seek 0.810ml @21:55:16.19 delay 3 seconds @21:55:19.20 ATV.dial :air3 @21:55:19.55 delay 3 seconds @21:55:22.61 AS.seek 0.835ml @21:55:27.97 delay 3 seconds @21:55:30.98 ATV.dial :bleach @21:55:31.38 AS.seek 0.820ml @21:55:34.69 delay 3 seconds @21:55:37.70 ARV.dial :waste @21:55:38.31 delay 3 seconds @21:55:41.32 ATV.dial :ARV @21:55:42.05 AS.reconfigure ASconfig @21:55:42.09 AS.seek 0.470ml @21:56:02.76 AFV.select :carrierSolution @21:56:02.85 delay 2 seconds @21:56:04.86 AS.seek 1.000ml @21:56:36.08 delay 1 second @21:56:37.09 AFV.select :holdingCoil @21:56:37.18 delay 1 second @21:56:38.19 AS.reconfigure ASfast @21:56:38.22 AS.seek 0.000ml @21:57:05.96 AFV.select :carrierSolution @21:57:06.05 delay 2 seconds @21:57:08.06 AS.reconfigure ASconfig @21:57:08.09 AS.seek 1.000ml @21:58:06.68 delay 1 second @21:58:07.69 AFV.select :holdingCoil @21:58:07.78 delay 1 second @21:58:08.79 AS.reconfigure ASfast @21:58:08.82 AS.seek 0.000ml @21:58:36.55 AFV.select :carrierSolution @21:58:36.64 delay 2 seconds @21:58:38.65 AS.reconfigure ASconfig @21:58:38.68 AS.seek 1.000ml @21:59:37.30 delay 1 second @21:59:38.31 AFV.select :holdingCoil @21:59:38.40 delay 1 second @21:59:39.41 AS.reconfigure ASfast @21:59:39.44 AS.seek 0.000ml @22:00:07.16 ARV.dial between carrier and air1 @22:00:07.58 ATV.dial :air3 @22:00:08.51 AS.reconfigure ASslow1 @22:00:08.55 AS.seek 0.010ml @22:00:10.87 delay 2 seconds @22:00:12.88 ATV.dial :bleach @22:00:13.36 AS.seek 0.810ml @22:02:56.76 delay 3 seconds @22:02:59.77 ATV.dial :air3 @22:03:00.10 delay 3 seconds @22:03:03.75 AS.seek 0.835ml @22:03:09.09 delay 3 seconds @22:03:12.10 ATV.dial :bleach @22:03:12.50 AS.seek 0.820ml @22:03:15.87 delay 3 seconds @22:03:18.88 ATV.dial PCR @22:03:19.32 AS.reconfigure ASconfig @22:03:19.35 AS.seek empty @22:04:07.45 PCR.program :bleachProfile @22:04:07.51 PCR program should complete in < 2:45 @22:04:07.52 PCR.run 10:00 @22:07:14.77 PCR.off @22:07:14.80 AFV.select :carrierSolution @22:07:14.89 delay 2 seconds @22:07:16.90 AS.seek 1.000ml @22:08:15.56 delay 1 second @22:08:16.57 AFV.select :holdingCoil @22:08:16.66 delay 1 second @22:08:17.67 AS.reconfigure ASfast @22:08:17.70 AS.seek 0.000ml @22:08:45.42 delay 3 seconds @22:08:48.43 AFV.select :carrierSolution @22:08:48.52 delay 2 seconds @22:08:50.53 AS.reconfigure ASconfig @22:08:50.56 AS.seek 1.000ml @22:09:49.17 delay 1 second @22:09:50.18 AFV.select :holdingCoil @22:09:50.27 delay 1 second @22:09:51.28 AS.reconfigure ASfast @22:09:51.31 AS.seek 0.000ml @22:10:19.04 delay 3 seconds @22:10:22.05 AFV.select :carrierSolution @22:10:22.14 delay 2 seconds @22:10:24.15 AS.reconfigure ASconfig @22:10:24.18 AS.seek 1.000ml @22:11:22.76 delay 1 second @22:11:23.77 AFV.select :holdingCoil @22:11:23.86 delay 1 second @22:11:24.87 AS.reconfigure ASfast @22:11:24.91 AS.seek 0.000ml @22:11:52.65 delay 3 seconds @22:11:55.66 AFV.select :carrierSolution @22:11:55.75 delay 2 seconds @22:11:57.76 AS.reconfigure ASconfig @22:11:57.79 AS.seek 1.000ml @22:12:56.37 delay 1 second @22:12:57.38 AFV.select :holdingCoil @22:12:57.47 delay 1 second @22:12:58.49 AS.reconfigure ASfast @22:12:58.52 AS.seek 0.000ml @22:13:26.22 delay 3 seconds @22:13:29.23 AFV.select :carrierSolution @22:13:29.32 delay 2 seconds @22:13:31.33 AS.reconfigure ASconfig @22:13:31.36 AS.seek 1.000ml @22:14:29.95 delay 1 second @22:14:30.96 AFV.select :holdingCoil @22:14:31.05 delay 1 second @22:14:32.06 AS.reconfigure ASnorm @22:14:32.09 AS.seek 0.000ml @22:15:23.55 AFV.select :carrierSolution @22:15:23.64 delay 2 seconds @22:15:25.65 AS.reconfigure ASconfig @22:15:25.68 AS.seek 1.000ml @22:16:24.26 delay 1 second @22:16:25.27 AFV.select :holdingCoil @22:16:25.36 delay 1 second @22:16:26.37 ATV.dial :waste @22:16:27.27 AS.reconfigure ASnorm @22:16:27.30 AS.seek 0.500ml @22:16:53.23 ATV.dial :waste2 @22:16:54.32 AS.seek 0.000ml @22:17:20.26
Cleaning PCR AFV.select :carrierSolution @22:17:20.35 delay 2 seconds @22:17:22.36 AS.reconfigure ASconfig @22:17:22.39 AS.seek 0.500ml @22:17:51.84 delay 1 second @22:17:52.86 AFV.select :holdingCoil @22:17:52.95 ASV.dial :waste1 @22:17:52.96 ATV.dial :air3 @22:17:54.44 AS.reconfigure ASslow1 @22:17:54.48 AS.seek 0.510ml @22:17:56.76 delay 2 seconds @22:17:58.77 ATV.dial :bleach @22:17:59.15 AS.seek 0.810ml @22:19:00.58 delay 3 seconds @22:19:03.59 ATV.dial :air3 @22:19:03.91 delay 3 seconds @22:19:06.97 AS.seek 0.835ml @22:19:12.30 delay 3 seconds @22:19:15.31 ATV.dial :bleach @22:19:15.79 AS.seek 0.820ml @22:19:19.08 delay 3 seconds @22:19:22.09 ARV.dial :waste @22:19:22.58 delay 3 seconds @22:19:25.59 ATV.dial :ARV @22:19:26.33 AS.reconfigure ASconfig @22:19:26.37 AS.seek 0.470ml @22:19:47.04 AFV.select :carrierSolution @22:19:47.13 delay 2 seconds @22:19:49.14 AS.seek 1.000ml @22:20:20.35 delay 1 second @22:20:21.36 AFV.select :holdingCoil @22:20:21.45 delay 1 second @22:20:22.46 AS.reconfigure ASfast @22:20:22.49 AS.seek 0.000ml @22:20:50.22 AFV.select :carrierSolution @22:20:50.31 delay 2 seconds @22:20:52.32 AS.reconfigure ASconfig @22:20:52.35 AS.seek 1.000ml @22:21:50.97 delay 1 second @22:21:51.98 AFV.select :holdingCoil @22:21:52.07 delay 1 second @22:21:53.08 AS.reconfigure ASfast @22:21:53.11 AS.seek 0.000ml @22:22:20.84 AFV.select :carrierSolution @22:22:20.93 delay 2 seconds @22:22:22.94 AS.reconfigure ASconfig @22:22:22.97 AS.seek 1.000ml @22:23:21.56 delay 1 second @22:23:22.57 AFV.select :holdingCoil @22:23:22.66 delay 1 second @22:23:23.67 AS.reconfigure ASfast @22:23:23.71 AS.seek 0.000ml @22:23:51.45 ARV.dial between carrier and air1 @22:23:51.97 ATV.dial :air3 @22:23:52.91 AS.reconfigure ASslow1 @22:23:52.95 AS.seek 0.010ml @22:23:55.29 delay 2 seconds @22:23:57.85 ATV.dial :bleach @22:23:58.25 AS.seek 0.810ml @22:26:41.62 delay 3 seconds @22:26:44.63 ATV.dial :air3 @22:26:45.10 delay 3 seconds @22:26:48.16 AS.seek 0.835ml @22:26:53.51 delay 3 seconds @22:26:56.52 ATV.dial :bleach @22:26:56.92 AS.seek 0.820ml @22:27:00.23 delay 3 seconds @22:27:03.24 ATV.dial PCR @22:27:03.56 AS.reconfigure ASconfig @22:27:03.59 AS.seek empty @22:27:51.71 PCR.program :bleachProfile @22:27:51.77 PCR program should complete in < 2:45 @22:27:51.78 PCR.run 10:00 @22:30:56.02 PCR.off @22:30:56.05 AFV.select :carrierSolution @22:30:56.14 delay 2 seconds @22:30:58.15 AS.seek 1.000ml @22:31:56.79 delay 1 second @22:31:57.80 AFV.select :holdingCoil @22:31:57.89 delay 1 second @22:31:58.90 AS.reconfigure ASfast @22:31:58.93 AS.seek 0.000ml @22:32:26.66 delay 3 seconds @22:32:29.67 AFV.select :carrierSolution @22:32:29.76 delay 2 seconds @22:32:31.77 AS.reconfigure ASconfig @22:32:31.80 AS.seek 1.000ml @22:33:30.39 delay 1 second @22:33:31.40 AFV.select :holdingCoil @22:33:31.49 delay 1 second @22:33:32.50 AS.reconfigure ASfast @22:33:32.53 AS.seek 0.000ml @22:34:00.27 delay 3 seconds @22:34:03.28 AFV.select :carrierSolution @22:34:03.37 delay 2 seconds @22:34:05.38 AS.reconfigure ASconfig @22:34:05.41 AS.seek 1.000ml @22:35:04.00 delay 1 second @22:35:05.01 AFV.select :holdingCoil @22:35:05.10 delay 1 second @22:35:06.11 AS.reconfigure ASfast @22:35:06.14 AS.seek 0.000ml @22:35:33.88 delay 3 seconds @22:35:36.89 AFV.select :carrierSolution @22:35:36.98 delay 2 seconds @22:35:38.99 AS.reconfigure ASconfig @22:35:39.02 AS.seek 1.000ml @22:36:37.62 delay 1 second @22:36:38.63 AFV.select :holdingCoil @22:36:38.72 delay 1 second @22:36:39.73 AS.reconfigure ASfast @22:36:39.80 AS.seek 0.000ml @22:37:07.53 delay 3 seconds @22:37:10.54 AFV.select :carrierSolution @22:37:10.63 delay 2 seconds @22:37:12.64 AS.reconfigure ASconfig @22:37:12.67 AS.seek 1.000ml @22:38:11.28 delay 1 second @22:38:12.29 AFV.select :holdingCoil @22:38:12.38 delay 1 second @22:38:13.39 AS.reconfigure ASnorm @22:38:13.42 AS.seek 0.000ml @22:39:04.87 Completed {wqPCR} begun 08:01:04 @22:39:04.90 Asleep til 08:01PDT13-Mar-14 @22:39:04.91 AS.stop @22:39:04.92
Sleeping until 08:01PDT13-Mar-14 @22:39:04.94 GoodNews.email "Then, in tube 3: wqPCR\n/home/esp/esp2local/mission/14mar05sccwrpDeploy.rb:64",Subject => "Asleep until 08:01PDT13-Mar-14" @22:39:07.01 CC.stop @22:39:07.03 CS.stop @22:39:07.06 PC.stop @22:39:07.08 PS.stop @22:39:07.11 Forearm.stop @22:39:07.13 Elbow.stop @22:39:07.16 SS.stop @22:39:07.23 Sleepy.power :raw,:OFF @22:39:07.24 Sleepy.power :dwarf,:OFF Shutdown @08:01:04.93PDT13-Mar-14
Powering up Dwarves @08:01:10.11 Sleepy.power :raw,:ON @08:01:10.57 SPE.configure SPEconfig @08:01:10.59 AS.configure ASconfig @08:01:10.62 CC.configure CCconfig @08:01:10.65 CS.configure CSconfig @08:01:10.68 PC.configure PCconfig @08:01:10.71 PS.configure PSconfig @08:01:10.74 Forearm.configure LooseForearm @08:01:10.77 Elbow.configure LooseElbow @08:01:10.80 SC.configure SCloose @08:01:10.83 SE.configure SEconfig @08:01:10.86 SS.configure SSconfig @08:01:10.89 CH.configure CHconfig @08:01:10.91 PH.configure PHconfig @08:01:10.94 Valve.reset @08:01:11.61 PCR.off @08:01:11.64 PCR.pidtemp= 50,0.1,0,300,25,25 @08:01:11.67 PCR.caltemp= 6.6661,0.0344,0.0 @08:01:11.78 Intake.select :close @08:01:11.79 Exhaust.select :close @08:01:11.80 CTV.dial :air @08:01:11.84 PSV.dial :air @08:01:11.87
PC.reconfigure PCconfig @08:01:11.93 PC.home.seek open @08:01:11.95 CSV.dial :waste1 @08:01:12.33 PS.reconfigure PSconfig @08:01:12.36 PS.home.seek 0.000ml @08:01:12.40 SSV.dial :spit,avoiding => exhaust @08:01:13.19 CS.reconfigure CSfast @08:01:13.22 SS.reconfigure SSconfig @08:01:13.31 SS.home.seek 0.00ml @08:01:13.33 CS.home.seek 0.000ml @08:01:13.41 CC.reconfigure CCconfig @08:01:13.45 CC.home.seek open @08:01:22.60 PS.coast @08:01:22.68
CC.seek open @08:01:22.70 PC.seek open @08:01:22.84
Forearm.reconfigure LooseForearm @08:01:22.87 Forearm.home.seek retract @08:01:23.63 Hand.grip @08:01:23.65
Elbow.jog -1500 @08:01:24.21 Elbow.reconfigure LooseElbow @08:01:24.25 Elbow.home.seek clear @08:01:28.95 SE.reconfigure SEconfig @08:01:28.98 SE.home.seek down @08:01:29.53 SC.reconfigure SCsloppy @08:01:29.57 SC.home.seek initial @08:01:33.27 Forearm.seek retract @08:01:33.42 Elbow.seek hammer @08:01:34.60 Forearm.reconfigure TightForearm @08:01:34.65 Forearm.seek 2 @08:01:35.67 Forearm.seek retract @08:01:36.77 Forearm.reconfigure LooseForearm @08:01:37.28PDT13-Mar-14 ===> STARTED wqPCR (phase #10) <=== @08:01:37.29 Starting initialPurge @08:01:37.30 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:01:37.35 Hand.release @08:01:38.14 Elbow.reconfigure TightElbow @08:01:38.18 Elbow.seek FlushPuck::Garage @08:01:39.95 Elbow.reconfigure LooseElbow @08:01:40.04 Forearm.seek garage @08:01:41.07 Hand.grip @08:01:41.89 Forearm.seek retract @08:01:42.91 Elbow.reconfigure TightElbow @08:01:42.95 Elbow.seek CC @08:01:45.72 Elbow.reconfigure LooseElbow @08:01:45.81 Forearm.reconfigure TightForearm @08:01:45.84 Forearm.seek CC @08:01:46.93 CC.seek closed @08:01:50.69 Collection Clamp onSpring/offSpring for 5/22 samples, on/off torque 1.93 @08:01:50.75 Hand.release @08:01:51.48 Forearm.reconfigure LooseForearm @08:01:51.51 Forearm.seek retract @08:01:52.48 Flushing the Collection Valve::Manifold @08:01:52.57 CS Pulling 5ml of flush @08:01:52.58 CSV.dial CSR @08:01:53.85 CSR.select :flush @08:01:53.95 CS.reconfigure CSconfig @08:01:53.99 CS.seek 5.000ml @08:02:07.48 delay 5 seconds @08:02:12.49 CSR.select :air @08:02:12.62 CS.reconfigure CSfast @08:02:12.66 CS.seek 7.500ml @08:02:19.35 CSV.dial :waste @08:02:20.60 CS.reconfigure CSconfig @08:02:20.64 CS.seek 0.000ml @08:02:40.48 Purging external Intake @08:02:40.57 CSV.dial :flush @08:02:41.01 CS.reconfigure CSfast @08:02:41.05 CS.seek 10.000ml @08:03:04.91 delay 5 seconds @08:03:09.92 CSV.dial CBV @08:03:09.94
CBV.dial CSV @08:03:11.15 CTV.dial :intake @08:03:11.90 Intake.select :open @08:03:11.99 CS.seek 0.000ml @08:03:36.35 Intake.select :close @08:03:36.44 CTV.dial :air @08:03:37.18 Flushing upper manifold @08:03:37.27 CSV.dial :flush @08:03:38.06 CS.seek 5.000ml @08:03:50.38 delay 5 seconds @08:03:55.39 CSV.dial :air @08:03:55.87 CS.seek 7.000ml @08:04:01.29 CSV.dial CBV @08:04:01.31
CBV.dial CSV @08:04:02.38 CTV.dial :air @08:04:02.40 CS.seek 0.000ml @08:04:19.22 delay 7 seconds @08:04:26.23 CS.seek 3.000ml @08:04:34.00 CSV.dial :waste @08:04:34.51 CS.seek 0.000ml @08:04:42.27 CSV.dial :flush @08:04:42.70 CS.seek 3.000ml @08:04:50.53 delay 5 seconds @08:04:55.54 CSV.dial :air @08:04:56.01 CS.seek 5.000ml @08:05:01.47 CSV.dial :air @08:05:01.48 CS.seek 0.000ml @08:05:13.63 delay 7 seconds @08:05:20.64 CS.seek 3.000ml @08:05:28.47 CSV.dial :waste @08:05:29.26 CS.seek 0.000ml @08:05:36.94 Garaging FlushPuck in Collection Clamp @08:05:37.00 Elbow.reconfigure TightElbow @08:05:37.03 Elbow.seek CC @08:05:37.24 Elbow.reconfigure LooseElbow @08:05:37.33 Forearm.seek CC @08:05:38.24 CC.seek sealed @08:05:39.19 Hand.grip @08:05:39.93 CC.seek open @08:05:43.22 Forearm.seek retract @08:05:44.17 Elbow.reconfigure TightElbow @08:05:44.21 Elbow.seek garage @08:05:47.63 Elbow.reconfigure LooseElbow @08:05:47.72 Forearm.reconfigure TightForearm @08:05:47.75 Forearm.seek garage @08:05:48.90 Hand.release @08:05:49.72 Forearm.reconfigure LooseForearm @08:05:49.75 Forearm.seek retract @08:05:50.87 Starting mfb @08:05:50.88 pcrBAC sampling at most 1000ml, wcr at most 1000ml @08:05:50.90 Loading sh1 puck from tube 3 into Collection Clamp @08:05:50.93 BACfans.email "Can@08:01:37, 15.6C, 67% humidity, 14.2psia, 13.719V, 0.598A, 0.591A avg, 8.20W\nCTD data unavailable\nISUS data unavailable",Subject => "pcrBAC sampling at most 1000ml, wcr at most 1000ml" @08:05:52.92 Readying the MFB @08:05:52.93 Homing MFB Syringe @08:05:52.94 AFV.select :holdingCoil @08:05:53.14
SC.reconfigure SCtight @08:05:53.18 SC.seek 3 @08:05:53.20 ATV.dial :air2 @08:05:54.52 AS.reconfigure ASconfig @08:05:54.55 AS.home.seek home @08:05:55.47
SC.reconfigure SCloose @08:05:55.65 Elbow.reconfigure TightElbow @08:05:55.68 Elbow.seek 3 @08:05:57.50 Forearm.reconfigure TightForearm @08:05:57.53 Forearm.seek 3 + 800 counts @08:05:58.51 SE.reconfigure SEpick @08:05:58.56 SE.seek beamBreak @08:05:59.92 SE.configure SEconfig @08:06:00.05 Elbow.seek 3 @08:06:00.21 Forearm.seek 3 + 300 counts @08:06:00.53 Forearm.reconfigure LooseForearm @08:06:00.57 Hand.grip @08:06:01.32 SE.seek down @08:06:01.45
Forearm.seek retract @08:06:02.03 AFV.select :carrierSolution @08:06:02.15 delay 2 seconds @08:06:02.40
Elbow.seek CC @08:06:02.50 SE.coast @08:06:03.98
Elbow.reconfigure LooseElbow @08:06:04.06 Forearm.reconfigure TightForearm @08:06:04.09 Forearm.seek CC @08:06:04.16 AS.seek 1.000ml @08:06:05.21
CC.seek closed @08:06:09.03 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.98 @08:06:09.04 Hand.release @08:06:09.84 Forearm.reconfigure LooseForearm @08:06:09.87 Forearm.seek retract @08:06:10.87 SC.coast @08:06:10.90 collectSample 1000ml w/bubble point of 55psi Priming 7ml @08:06:10.91 Sleepy.power :sampler,:ON @08:06:10.92 Intake.select :close Exhaust.select :close @08:06:10.93 SS.hold @08:06:11.09 Calibrating pressure and temperature sensors @08:06:11.10 CTV.dial :air @08:06:11.11 SSV.dial :spit,avoiding => exhaust @08:06:11.13 SS.reconfigure SSfast @08:06:11.16 SS.seek 5.00ml @08:06:14.21 SSV.dial CBV @08:06:14.23
CBV.dial SSV @08:06:15.62 delay 3.8 seconds @08:06:19.50 SSV.dial :spit @08:06:20.85 SS.seek 0.00ml @08:06:23.34 SSV.dial CBV @08:06:24.68 CTV.dial :intake @08:06:25.44 Waiting for external sampler to finish priming... @08:06:25.45 delayUntil "08:16:10.91" @08:06:55.87 delay 1 second @08:06:57.43 AFV.select :holdingCoil @08:06:57.52 delay 1 second @08:06:58.53 ATV.dial :waste @08:06:59.44 AS.reconfigure ASnorm @08:06:59.47 AS.home.seek empty @08:07:52.73 ATV.dial :waste @08:07:52.74 ARV.dial between carrier and air1 @08:07:52.81 ASV.dial :waste1 @08:16:10.92 SSV.dial CBV @08:16:10.94
CBV.dial SSV @08:16:10.95 Allowing 55s for 7.0ml of remaining 7.0ml Intake.select :open @08:16:13.08 Intake @57.1psia, vacuum from 20.0 to 24.0psi @08:16:13.09 SS.reconfigure forSampling @08:16:13.13 SS.seek 7.00ml @08:16:19.49 Stroke completed -- waiting for pressure to equalize ... @08:16:20.14 Intake.select :close @08:16:20.27 SSV.dial :exhaust @08:16:21.04 Exhaust.select :open @08:16:21.13 SS.reconfigure SSexhale @08:16:21.17 SS.seek 0.00ml @08:16:24.82 Exhaust.select :close @08:16:24.92 Sampling 1000.0ml @08:16:24.93 Intake.select :close @08:16:24.94 Exhaust.select :close @08:16:24.95 SS.reconfigure SSconfig @08:16:24.98 SS.hold @08:16:25.14 CTV.dial :intake @08:16:25.15 SSV.dial CBV @08:16:25.17
CBV.dial SSV @08:16:25.94 Allowing 185s for 25.0ml of remaining 1000.0ml @08:16:25.95 Intake.select :open @08:16:28.10 Intake @57.2psia, vacuum from 20.0 to 24.0psi @08:16:28.11 SS.reconfigure forSampling @08:16:28.15 SS.seek 25.00ml @08:16:43.13 Stroke completed -- waiting for pressure to equalize ... @08:16:43.77 Intake.select :close @08:16:43.90 SSV.dial :exhaust @08:16:44.68 Exhaust.select :open @08:16:44.77 SS.reconfigure SSexhale @08:16:44.81 SS.seek 0.00ml @08:16:53.83 Exhaust.select :close @08:16:53.92 SSV.dial CBV @08:16:53.94
CBV.dial SSV @08:16:54.72 Allowing 185s for 25.0ml of remaining 975.0ml @08:16:54.73 Intake.select :open @08:16:56.86 Intake @57.1psia, vacuum from 20.0 to 24.0psi @08:16:56.87 SS.reconfigure forSampling @08:16:56.91 SS.seek 25.00ml @08:17:08.36 Vacuum too high -- waiting up to 40s @08:17:08.62 Intake @50.1psia, vacuum from 20.0 to 24.0psi @08:17:08.63 SS.reconfigure forSampling @08:17:08.67 SS.seek 25.00ml @08:17:12.88 Stroke completed -- waiting for pressure to equalize ... @08:17:13.52 Intake.select :close @08:17:13.65 SSV.dial :exhaust @08:17:14.43 Exhaust.select :open @08:17:14.52 SS.reconfigure SSexhale @08:17:14.56 SS.seek 0.00ml @08:17:23.52 Exhaust.select :close @08:17:23.61 SSV.dial CBV @08:17:23.63
CBV.dial SSV @08:17:24.41 Allowing 185s for 25.0ml of remaining 950.0ml @08:17:24.42 Intake.select :open @08:17:26.55 Intake @56.9psia, vacuum from 20.0 to 24.0psi @08:17:26.56 SS.reconfigure forSampling @08:17:26.60 SS.seek 25.00ml @08:17:32.73 Vacuum too high -- waiting up to 40s @08:17:32.99 Intake @51.7psia, vacuum from 20.0 to 24.0psi @08:17:33.00 SS.reconfigure forSampling @08:17:33.04 SS.seek 25.00ml @08:17:44.22 Stroke completed -- waiting for pressure to equalize ... @08:17:44.85 Intake.select :close @08:17:45.00 SSV.dial :exhaust @08:17:45.78 Exhaust.select :open @08:17:45.87 SS.reconfigure SSexhale @08:17:45.91 SS.seek 0.00ml @08:17:54.94 Exhaust.select :close @08:17:55.03 SSV.dial CBV @08:17:55.05
CBV.dial SSV @08:17:55.81 Allowing 185s for 25.0ml of remaining 925.0ml @08:17:55.82 Intake.select :open @08:17:57.95 Intake @56.4psia, vacuum from 20.0 to 24.0psi @08:17:57.96 SS.reconfigure forSampling @08:17:58.00 SS.seek 25.00ml @08:18:01.64 Vacuum too high -- waiting up to 40s @08:18:01.90 Intake @53.5psia, vacuum from 20.0 to 24.0psi @08:18:01.91 SS.reconfigure forSampling @08:18:01.95 SS.seek 25.00ml @08:18:04.27 Vacuum too high -- waiting up to 40s @08:18:04.52 Intake @53.1psia, vacuum from 20.0 to 24.0psi @08:18:04.53 SS.reconfigure forSampling @08:18:04.57 SS.seek 25.00ml @08:18:09.20 Vacuum too high -- waiting up to 40s @08:18:09.46 Intake @53.6psia, vacuum from 20.0 to 24.0psi @08:18:09.47 SS.reconfigure forSampling @08:18:09.51 SS.seek 25.00ml @08:18:16.73 Vacuum too high -- waiting up to 40s @08:18:16.99 Intake @55.0psia, vacuum from 20.0 to 24.0psi @08:18:17.00 SS.reconfigure forSampling @08:18:17.04 SS.seek 25.00ml @08:18:22.48 Vacuum too high -- waiting up to 40s @08:18:22.75 Intake @55.3psia, vacuum from 20.0 to 24.0psi @08:18:22.76 SS.reconfigure forSampling @08:18:22.79 SS.seek 25.00ml @08:18:28.66 Stroke completed -- waiting for pressure to equalize ... @08:18:29.30 Intake.select :close @08:18:29.43 SSV.dial :exhaust @08:18:30.22 Exhaust.select :open @08:18:30.31 SS.reconfigure SSexhale @08:18:30.35 SS.seek 0.00ml @08:18:39.11 Exhaust.select :close @08:18:39.70 SSV.dial CBV @08:18:39.72
CBV.dial SSV @08:18:40.59 Allowing 185s for 25.0ml of remaining 900.0ml Intake.select :open @08:18:42.73 Intake @56.3psia, vacuum from 20.0 to 24.0psi @08:18:42.74 SS.reconfigure forSampling @08:18:42.78 SS.seek 25.00ml @08:18:55.04 Vacuum too high -- waiting up to 40s @08:18:55.31 Intake @55.9psia, vacuum from 20.0 to 24.0psi @08:18:55.32 SS.reconfigure forSampling @08:18:55.35 SS.seek 25.00ml @08:19:38.23 Stroke completed -- waiting for pressure to equalize ... @08:19:38.87 Intake.select :close @08:19:39.00 SSV.dial :exhaust @08:19:39.78 Exhaust.select :open @08:19:39.88 SS.reconfigure SSexhale @08:19:39.92 SS.seek 0.00ml @08:19:48.56 Exhaust.select :close @08:19:48.65 SSV.dial CBV @08:19:48.67
CBV.dial SSV @08:19:49.43 Allowing 185s for 25.0ml of remaining 875.0ml @08:19:49.44 Intake.select :open @08:19:51.57 Intake @56.5psia, vacuum from 20.0 to 24.0psi @08:19:51.58 SS.reconfigure forSampling @08:19:51.62 SS.seek 25.00ml @08:19:54.39 Vacuum too high -- waiting up to 40s @08:19:54.65 Intake @55.9psia, vacuum from 20.0 to 24.0psi @08:19:54.66 SS.reconfigure forSampling @08:19:54.69 SS.seek 25.00ml @08:21:40.57 Stroke completed -- waiting for pressure to equalize ... @08:21:41.79 Intake.select :close @08:21:41.92 SSV.dial :exhaust @08:21:42.69 Exhaust.select :open @08:21:42.79 SS.reconfigure SSexhale @08:21:42.83 SS.seek 0.00ml @08:21:51.96 Exhaust.select :close @08:21:52.05 SSV.dial CBV @08:21:52.07
CBV.dial SSV @08:21:52.85 Allowing 185s for 25.0ml of remaining 850.0ml @08:21:52.86 Intake.select :open @08:21:55.00 Intake @56.2psia, vacuum from 20.0 to 24.0psi @08:21:55.01 SS.reconfigure forSampling @08:21:55.04 SS.seek 25.00ml @08:22:51.06 Vacuum too high -- waiting up to 40s @08:22:51.32 Intake @56.1psia, vacuum from 20.0 to 24.0psi @08:22:51.33 SS.reconfigure forSampling @08:22:51.37 SS.seek 25.00ml @08:23:02.37 Vacuum too high -- waiting up to 40s @08:23:02.64 Intake @55.9psia, vacuum from 20.0 to 24.0psi @08:23:02.65 SS.reconfigure forSampling @08:23:02.68 SS.seek 25.00ml @08:23:05.97 Vacuum too high -- waiting up to 40s @08:23:06.23 Intake @55.6psia, vacuum from 20.0 to 24.0psi @08:23:06.24 SS.reconfigure forSampling @08:23:06.28 SS.seek 25.00ml @08:23:14.68 Vacuum too high -- waiting up to 40s @08:23:14.95 Intake @56.1psia, vacuum from 20.0 to 24.0psi @08:23:14.96 SS.reconfigure forSampling @08:23:14.99 SS.seek 25.00ml @08:23:17.19 Vacuum too high -- waiting up to 40s @08:23:17.45 Intake @55.7psia, vacuum from 20.0 to 24.0psi @08:23:17.46 SS.reconfigure forSampling @08:23:17.50 SS.seek 25.00ml @08:23:19.32 Vacuum too high -- waiting up to 40s @08:23:19.57 Intake @55.5psia, vacuum from 20.0 to 24.0psi @08:23:19.58 SS.reconfigure forSampling @08:23:19.62 SS.seek 25.00ml @08:23:22.04 Vacuum too high -- waiting up to 40s @08:23:22.30 Intake @55.6psia, vacuum from 20.0 to 24.0psi @08:23:22.31 SS.reconfigure forSampling @08:23:22.34 SS.seek 25.00ml @08:23:24.51 Vacuum too high -- waiting up to 40s @08:23:24.77 Intake @55.7psia, vacuum from 20.0 to 24.0psi @08:23:24.78 SS.reconfigure forSampling @08:23:24.81 SS.seek 25.00ml @08:23:26.60 Vacuum too high -- waiting up to 40s @08:23:26.85 Intake @55.8psia, vacuum from 20.0 to 24.0psi @08:23:26.86 SS.reconfigure forSampling @08:23:26.90 SS.seek 25.00ml @08:23:30.37 Vacuum too high -- waiting up to 40s @08:23:30.64 Intake @55.9psia, vacuum from 20.0 to 24.0psi @08:23:30.65 SS.reconfigure forSampling @08:23:30.68 SS.seek 25.00ml @08:23:32.41 Vacuum too high -- waiting up to 40s @08:23:32.66 Intake @55.8psia, vacuum from 20.0 to 24.0psi @08:23:32.67 SS.reconfigure forSampling @08:23:32.71 SS.seek 25.00ml @08:23:34.13 Vacuum too high -- waiting up to 40s @08:23:34.38 Intake @55.4psia, vacuum from 20.0 to 24.0psi @08:23:34.39 SS.reconfigure forSampling @08:23:34.43 SS.seek 25.00ml @08:23:36.44 Vacuum too high -- waiting up to 40s @08:23:36.70 Intake @55.9psia, vacuum from 20.0 to 24.0psi @08:23:36.71 SS.reconfigure forSampling @08:23:36.74 SS.seek 25.00ml @08:23:38.69 Vacuum too high -- waiting up to 40s @08:23:38.95 SS.seek 25.00ml @08:23:40.25 Vacuum too high -- waiting up to 40s @08:23:40.51 Intake @55.7psia, vacuum from 20.0 to 24.0psi @08:23:40.52 SS.reconfigure forSampling @08:23:40.55 SS.seek 25.00ml @08:23:42.47 Vacuum too high -- waiting up to 40s @08:23:42.74 Intake @55.6psia, vacuum from 20.0 to 24.0psi @08:23:42.75 SS.reconfigure forSampling @08:23:42.78 SS.seek 25.00ml @08:23:45.41 Vacuum too high -- waiting up to 40s @08:23:45.67 Intake @55.9psia, vacuum from 20.0 to 24.0psi @08:23:45.68 SS.reconfigure forSampling @08:23:45.71 SS.seek 25.00ml @08:23:47.32 Vacuum too high -- waiting up to 40s @08:23:47.58 Intake @55.8psia, vacuum from 20.0 to 24.0psi @08:23:47.59 SS.reconfigure forSampling @08:23:47.63 SS.seek 25.00ml @08:23:49.10 Vacuum too high -- waiting up to 40s @08:23:49.37 Intake @55.4psia, vacuum from 20.0 to 24.0psi @08:23:49.38 SS.reconfigure forSampling @08:23:49.41 SS.seek 25.00ml @08:23:51.25 Vacuum too high -- waiting up to 40s @08:23:51.51 Intake @55.8psia, vacuum from 20.0 to 24.0psi @08:23:51.52 SS.reconfigure forSampling @08:23:51.56 SS.seek 25.00ml @08:23:53.60 Vacuum too high -- waiting up to 40s @08:23:53.86 Intake @56.1psia, vacuum from 20.0 to 24.0psi @08:23:53.87 SS.reconfigure forSampling @08:23:53.90 SS.seek 25.00ml @08:23:55.92 Stroke completed -- waiting for pressure to equalize ... @08:23:57.73 Intake.select :close @08:23:57.86 SSV.dial :exhaust @08:23:58.64 Exhaust.select :open @08:23:58.73 SS.reconfigure SSexhale @08:23:58.77 SS.seek 0.00ml @08:24:07.56 Exhaust.select :close @08:24:07.65 SSV.dial CBV @08:24:07.67
CBV.dial SSV @08:24:08.47 Allowing 185s for 25.0ml of remaining 825.0ml @08:24:08.48 Intake.select :open @08:24:10.62 Intake @56.0psia, vacuum from 20.0 to 24.0psi @08:24:10.63 SS.reconfigure forSampling @08:24:10.67 SS.seek 25.00ml @08:24:15.34 Vacuum too high -- waiting up to 40s @08:24:15.60 Intake @55.6psia, vacuum from 20.0 to 24.0psi @08:24:15.61 SS.reconfigure forSampling @08:24:15.65 SS.seek 25.00ml @08:24:17.78 Vacuum too high -- waiting up to 40s @08:24:18.04 SS.seek 25.00ml @08:24:19.88 Vacuum too high -- waiting up to 40s @08:24:20.14 Intake @55.8psia, vacuum from 20.0 to 24.0psi @08:24:20.15 SS.reconfigure forSampling @08:24:20.19 SS.seek 25.00ml @08:24:21.91 Vacuum too high -- waiting up to 40s @08:24:22.16 Intake @55.5psia, vacuum from 20.0 to 24.0psi @08:24:22.17 SS.reconfigure forSampling @08:24:22.21 SS.seek 25.00ml @08:24:24.38 Vacuum too high -- waiting up to 40s @08:24:24.63 Intake @55.8psia, vacuum from 20.0 to 24.0psi @08:24:24.64 SS.reconfigure forSampling @08:24:24.68 SS.seek 25.00ml @08:24:26.57 Vacuum too high -- waiting up to 40s @08:24:26.82 Intake @55.7psia, vacuum from 20.0 to 24.0psi @08:24:26.83 SS.reconfigure forSampling @08:24:26.87 SS.seek 25.00ml @08:24:28.57 Vacuum too high -- waiting up to 40s @08:24:28.82 Intake @55.4psia, vacuum from 20.0 to 24.0psi @08:24:28.83 SS.reconfigure forSampling @08:24:28.87 SS.seek 25.00ml @08:24:30.38 Vacuum too high -- waiting up to 40s @08:24:30.63 Intake @55.7psia, vacuum from 20.0 to 24.0psi @08:24:30.64 SS.reconfigure forSampling @08:24:30.67 SS.seek 25.00ml @08:24:31.94 Vacuum too high -- waiting up to 40s @08:24:32.20 SS.seek 25.00ml @08:24:33.81 Vacuum too high -- waiting up to 40s @08:24:34.06 Intake @56.0psia, vacuum from 20.0 to 24.0psi @08:24:34.07 SS.reconfigure forSampling @08:24:34.11 SS.seek 25.00ml @08:24:35.66 Vacuum too high -- waiting up to 40s @08:24:35.91 Intake @56.2psia, vacuum from 20.0 to 24.0psi @08:24:35.92 SS.reconfigure forSampling @08:24:35.96 SS.seek 25.00ml @08:24:37.44 Vacuum too high -- waiting up to 40s @08:24:37.69 Intake @55.9psia, vacuum from 20.0 to 24.0psi @08:24:37.70 SS.reconfigure forSampling @08:24:37.74 SS.seek 25.00ml @08:24:38.91 Vacuum too high -- waiting up to 40s @08:24:39.16 Intake @55.5psia, vacuum from 20.0 to 24.0psi @08:24:39.17 SS.reconfigure forSampling @08:24:39.21 SS.seek 25.00ml @08:24:40.56 Vacuum too high -- waiting up to 40s @08:24:40.82 Intake @55.2psia, vacuum from 20.0 to 24.0psi @08:24:40.83 SS.reconfigure forSampling @08:24:40.86 SS.seek 25.00ml @08:24:42.03 Vacuum too high -- waiting up to 40s @08:24:42.29 Intake @55.4psia, vacuum from 20.0 to 24.0psi @08:24:42.30 SS.reconfigure forSampling @08:24:42.33 SS.seek 25.00ml @08:24:43.72 Vacuum too high -- waiting up to 40s @08:24:44.59 Intake @55.5psia, vacuum from 20.0 to 24.0psi @08:24:44.60 SS.reconfigure forSampling @08:24:44.63 SS.seek 25.00ml @08:24:46.66 Vacuum too high -- waiting up to 40s @08:24:46.92 Intake @55.7psia, vacuum from 20.0 to 24.0psi @08:24:46.93 SS.reconfigure forSampling @08:24:46.96 SS.seek 25.00ml @08:24:48.22 Vacuum too high -- waiting up to 40s @08:24:48.48 Intake @55.4psia, vacuum from 20.0 to 24.0psi @08:24:48.49 SS.reconfigure forSampling @08:24:48.52 SS.seek 25.00ml @08:24:50.00 Vacuum too high -- waiting up to 40s @08:24:50.26 Intake @55.6psia, vacuum from 20.0 to 24.0psi @08:24:50.27 SS.reconfigure forSampling @08:24:50.30 SS.seek 25.00ml @08:24:51.53 Vacuum too high -- waiting up to 40s @08:24:51.79 Intake @55.9psia, vacuum from 20.0 to 24.0psi @08:24:51.80 SS.reconfigure forSampling @08:24:51.83 SS.seek 25.00ml @08:24:53.10 Vacuum too high -- waiting up to 40s @08:24:53.36 Intake @56.0psia, vacuum from 20.0 to 24.0psi @08:24:53.37 SS.reconfigure forSampling @08:24:53.40 SS.seek 25.00ml @08:24:54.72 Vacuum too high -- waiting up to 40s @08:24:54.98 Intake @56.3psia, vacuum from 20.0 to 24.0psi @08:24:54.99 SS.reconfigure forSampling @08:24:55.02 SS.seek 25.00ml @08:24:56.16 Vacuum too high -- waiting up to 40s @08:24:56.42 Intake @55.9psia, vacuum from 20.0 to 24.0psi @08:24:56.43 SS.reconfigure forSampling @08:24:56.46 SS.seek 25.00ml @08:24:57.59 Vacuum too high -- waiting up to 40s @08:24:57.85 Intake @55.3psia, vacuum from 20.0 to 24.0psi @08:24:57.86 SS.reconfigure forSampling @08:24:57.89 SS.seek 25.00ml @08:24:59.28 Vacuum too high -- waiting up to 40s @08:24:59.54 Intake @55.1psia, vacuum from 20.0 to 24.0psi @08:24:59.55 SS.reconfigure forSampling @08:24:59.59 SS.seek 25.00ml @08:25:00.81 Vacuum too high -- waiting up to 40s @08:25:01.07 Intake @55.2psia, vacuum from 20.0 to 24.0psi @08:25:01.08 SS.reconfigure forSampling @08:25:01.11 SS.seek 25.00ml @08:25:02.25 Vacuum too high -- waiting up to 40s @08:25:02.50 Intake @55.4psia, vacuum from 20.0 to 24.0psi @08:25:02.51 SS.reconfigure forSampling @08:25:02.55 SS.seek 25.00ml @08:25:03.78 Vacuum too high -- waiting up to 40s @08:25:04.04 Intake @55.6psia, vacuum from 20.0 to 24.0psi @08:25:04.05 SS.reconfigure forSampling @08:25:04.08 SS.seek 25.00ml @08:25:05.16 Vacuum too high -- waiting up to 40s @08:25:05.41 Intake @55.5psia, vacuum from 20.0 to 24.0psi @08:25:05.42 SS.reconfigure forSampling @08:25:05.46 SS.seek 25.00ml @08:25:06.50 Vacuum too high -- waiting up to 40s @08:25:06.76 Intake @55.5psia, vacuum from 20.0 to 24.0psi @08:25:06.77 SS.reconfigure forSampling @08:25:06.80 SS.seek 25.00ml @08:25:07.97 Vacuum too high -- waiting up to 40s @08:25:08.22 Intake @55.9psia, vacuum from 20.0 to 24.0psi @08:25:08.23 SS.reconfigure forSampling @08:25:08.27 SS.seek 25.00ml @08:25:09.38 Vacuum too high -- waiting up to 40s @08:25:09.63 Intake @56.0psia, vacuum from 20.0 to 24.0psi @08:25:09.64 SS.reconfigure forSampling @08:25:09.68 SS.seek 25.00ml @08:25:10.56 Vacuum too high -- waiting up to 40s @08:25:10.82 Intake @55.9psia, vacuum from 20.0 to 24.0psi @08:25:10.83 SS.reconfigure forSampling @08:25:10.86 SS.seek 25.00ml @08:25:11.81 Vacuum too high -- waiting up to 40s @08:25:12.06 Intake @56.0psia, vacuum from 20.0 to 24.0psi @08:25:12.07 SS.reconfigure forSampling @08:25:12.11 SS.seek 25.00ml @08:25:13.13 Vacuum too high -- waiting up to 40s @08:25:13.40 SS.seek 25.00ml @08:25:14.41 Vacuum too high -- waiting up to 40s @08:25:14.66 Intake @55.8psia, vacuum from 20.0 to 24.0psi @08:25:14.67 SS.reconfigure forSampling @08:25:14.71 SS.seek 25.00ml @08:25:15.94 Vacuum too high -- waiting up to 40s @08:25:16.41 Intake @55.8psia, vacuum from 20.0 to 24.0psi @08:25:16.42 SS.reconfigure forSampling @08:25:16.45 SS.seek 25.00ml @08:25:17.84 Vacuum too high -- waiting up to 40s @08:25:18.09 Intake @55.6psia, vacuum from 20.0 to 24.0psi @08:25:18.10 SS.reconfigure forSampling @08:25:18.14 SS.seek 25.00ml @08:25:19.19 Vacuum too high -- waiting up to 40s @08:25:19.45 Intake @55.4psia, vacuum from 20.0 to 24.0psi @08:25:19.46 SS.reconfigure forSampling @08:25:19.49 SS.seek 25.00ml @08:25:20.90 Vacuum too high -- waiting up to 40s @08:25:21.79 Intake @55.9psia, vacuum from 20.0 to 24.0psi @08:25:21.80 SS.reconfigure forSampling @08:25:21.84 SS.seek 25.00ml @08:25:23.62 Vacuum too high -- waiting up to 40s @08:25:23.88 Intake @56.2psia, vacuum from 20.0 to 24.0psi @08:25:23.89 SS.reconfigure forSampling @08:25:23.92 SS.seek 25.00ml @08:25:24.81 Vacuum too high -- waiting up to 40s @08:25:25.07 Intake @56.2psia, vacuum from 20.0 to 24.0psi @08:25:25.08 SS.reconfigure forSampling @08:25:25.12 SS.seek 25.00ml @08:25:26.09 Vacuum too high -- waiting up to 40s @08:25:26.35 Intake @55.8psia, vacuum from 20.0 to 24.0psi @08:25:26.36 SS.reconfigure forSampling @08:25:26.39 SS.seek 25.00ml @08:25:27.37 Vacuum too high -- waiting up to 40s @08:25:27.63 Intake @55.4psia, vacuum from 20.0 to 24.0psi @08:25:27.64 SS.reconfigure forSampling @08:25:27.67 SS.seek 25.00ml @08:25:28.72 Vacuum too high -- waiting up to 40s @08:25:28.98 Intake @55.6psia, vacuum from 20.0 to 24.0psi @08:25:28.99 SS.reconfigure forSampling @08:25:29.02 SS.seek 25.00ml @08:25:30.06 Vacuum too high -- waiting up to 40s @08:25:30.32 Intake @55.7psia, vacuum from 20.0 to 24.0psi @08:25:30.33 SS.reconfigure forSampling @08:25:30.36 SS.seek 25.00ml @08:25:31.47 Vacuum too high -- waiting up to 40s @08:25:31.73 Intake @55.8psia, vacuum from 20.0 to 24.0psi @08:25:31.74 SS.reconfigure forSampling @08:25:31.77 SS.seek 25.00ml @08:25:32.97 Vacuum too high -- waiting up to 40s @08:25:33.45 Intake @56.5psia, vacuum from 20.0 to 24.0psi @08:25:33.46 SS.reconfigure forSampling @08:25:33.49 SS.seek 25.00ml @08:25:34.91 Vacuum too high -- waiting up to 40s @08:25:35.37 Intake @56.2psia, vacuum from 20.0 to 24.0psi @08:25:35.38 SS.reconfigure forSampling @08:25:35.42 SS.seek 25.00ml @08:25:36.81 Vacuum too high -- waiting up to 40s @08:25:37.06 Intake @55.8psia, vacuum from 20.0 to 24.0psi @08:25:37.07 SS.reconfigure forSampling @08:25:37.11 SS.seek 25.00ml @08:25:38.09 Vacuum too high -- waiting up to 40s @08:25:38.64 Intake @55.8psia, vacuum from 20.0 to 24.0psi @08:25:38.65 SS.reconfigure forSampling @08:25:38.69 SS.seek 25.00ml @08:25:40.09 Vacuum too high -- waiting up to 40s @08:25:40.34 Intake @55.4psia, vacuum from 20.0 to 24.0psi @08:25:40.35 SS.reconfigure forSampling @08:25:40.39 SS.seek 25.00ml @08:25:41.28 Vacuum too high -- waiting up to 40s @08:25:41.54 Intake @55.1psia, vacuum from 20.0 to 24.0psi @08:25:41.55 SS.reconfigure forSampling @08:25:41.58 SS.seek 25.00ml @08:25:42.65 Vacuum too high -- waiting up to 40s @08:25:42.91 Intake @55.3psia, vacuum from 20.0 to 24.0psi @08:25:42.92 SS.reconfigure forSampling @08:25:42.95 SS.seek 25.00ml @08:25:43.72 Vacuum too high -- waiting up to 40s @08:25:43.98 Intake @55.0psia, vacuum from 20.0 to 24.0psi @08:25:43.99 SS.reconfigure forSampling @08:25:44.02 SS.seek 25.00ml @08:25:45.06 Vacuum too high -- waiting up to 40s @08:25:45.32 Intake @54.8psia, vacuum from 20.0 to 24.0psi @08:25:45.33 SS.reconfigure forSampling @08:25:45.36 SS.seek 25.00ml @08:25:46.40 Vacuum too high -- waiting up to 40s @08:25:46.66 Intake @55.0psia, vacuum from 20.0 to 24.0psi @08:25:46.67 SS.reconfigure forSampling @08:25:46.70 SS.seek 25.00ml @08:25:47.75 Vacuum too high -- waiting up to 40s @08:25:48.21 Intake @56.5psia, vacuum from 20.0 to 24.0psi @08:25:48.22 SS.reconfigure forSampling @08:25:48.26 SS.seek 25.00ml @08:25:49.72 Vacuum too high -- waiting up to 40s @08:25:49.98 Intake @56.2psia, vacuum from 20.0 to 24.0psi @08:25:49.99 SS.reconfigure forSampling @08:25:50.03 SS.seek 25.00ml @08:25:51.12 Vacuum too high -- waiting up to 40s @08:25:51.37 Intake @55.2psia, vacuum from 20.0 to 24.0psi @08:25:51.38 SS.reconfigure forSampling @08:25:51.42 SS.seek 25.00ml @08:25:52.37 Vacuum too high -- waiting up to 40s @08:25:52.62 Intake @55.5psia, vacuum from 20.0 to 24.0psi @08:25:52.63 SS.reconfigure forSampling @08:25:52.67 SS.seek 25.00ml @08:25:53.50 Vacuum too high -- waiting up to 40s @08:25:53.75 Intake @55.8psia, vacuum from 20.0 to 24.0psi @08:25:53.76 SS.reconfigure forSampling @08:25:53.80 SS.seek 25.00ml @08:25:54.72 Vacuum too high -- waiting up to 40s @08:25:55.82 Intake @55.9psia, vacuum from 20.0 to 24.0psi @08:25:55.83 SS.reconfigure forSampling @08:25:55.86 SS.seek 25.00ml @08:25:57.37 Vacuum too high -- waiting up to 40s @08:25:57.63 Intake @55.6psia, vacuum from 20.0 to 24.0psi @08:25:57.64 SS.reconfigure forSampling @08:25:57.67 SS.seek 25.00ml @08:25:58.65 Vacuum too high -- waiting up to 40s @08:25:58.91 Intake @55.7psia, vacuum from 20.0 to 24.0psi @08:25:58.92 SS.reconfigure forSampling @08:25:58.96 SS.seek 25.00ml @08:25:59.97 Vacuum too high -- waiting up to 40s @08:26:00.25 Intake @55.5psia, vacuum from 20.0 to 24.0psi @08:26:00.26 SS.reconfigure forSampling @08:26:00.29 SS.seek 25.00ml @08:26:01.15 Vacuum too high -- waiting up to 40s @08:26:01.63 Intake @55.5psia, vacuum from 20.0 to 24.0psi @08:26:01.64 SS.reconfigure forSampling @08:26:01.67 SS.seek 25.00ml @08:26:02.81 Vacuum too high -- waiting up to 40s @08:26:03.28 Intake @55.3psia, vacuum from 20.0 to 24.0psi @08:26:03.29 SS.reconfigure forSampling @08:26:03.32 SS.seek 25.00ml @08:26:04.72 Vacuum too high -- waiting up to 40s @08:26:05.19 Intake @55.7psia, vacuum from 20.0 to 24.0psi @08:26:05.20 SS.reconfigure forSampling @08:26:05.23 SS.seek 25.00ml @08:26:06.68 Vacuum too high -- waiting up to 40s @08:26:07.15 Intake @56.9psia, vacuum from 20.0 to 24.0psi @08:26:07.16 SS.reconfigure forSampling @08:26:07.19 SS.seek 25.00ml @08:26:08.40 Vacuum too high -- waiting up to 40s @08:26:08.65 Intake @56.5psia, vacuum from 20.0 to 24.0psi @08:26:08.66 SS.reconfigure forSampling @08:26:08.70 SS.seek 25.00ml @08:26:09.53 Vacuum too high -- waiting up to 40s @08:26:09.79 Intake @56.0psia, vacuum from 20.0 to 24.0psi @08:26:09.80 SS.reconfigure forSampling @08:26:09.83 SS.seek 25.00ml @08:26:10.90 Vacuum too high -- waiting up to 40s @08:26:11.16 Intake @56.0psia, vacuum from 20.0 to 24.0psi @08:26:11.17 SS.reconfigure forSampling @08:26:11.20 SS.seek 25.00ml @08:26:12.15 Vacuum too high -- waiting up to 40s @08:26:12.41 Intake @56.0psia, vacuum from 20.0 to 24.0psi @08:26:12.42 SS.reconfigure forSampling @08:26:12.45 SS.seek 25.00ml @08:26:13.34 Vacuum too high -- waiting up to 40s @08:26:13.86 Intake @55.8psia, vacuum from 20.0 to 24.0psi @08:26:13.87 SS.reconfigure forSampling @08:26:13.91 SS.seek 25.00ml @08:26:15.12 Vacuum too high -- waiting up to 40s @08:26:15.38 Intake @55.7psia, vacuum from 20.0 to 24.0psi @08:26:15.39 SS.reconfigure forSampling @08:26:15.42 SS.seek 25.00ml @08:26:16.34 Vacuum too high -- waiting up to 40s @08:26:16.60 Intake @55.6psia, vacuum from 20.0 to 24.0psi @08:26:16.61 SS.reconfigure forSampling @08:26:16.64 SS.seek 25.00ml @08:26:17.43 Vacuum too high -- waiting up to 40s @08:26:17.69 Intake @55.3psia, vacuum from 20.0 to 24.0psi @08:26:17.70 SS.reconfigure forSampling @08:26:17.73 SS.seek 25.00ml @08:26:18.72 Vacuum too high -- waiting up to 40s @08:26:19.19 Intake @55.0psia, vacuum from 20.0 to 24.0psi @08:26:19.20 SS.reconfigure forSampling @08:26:19.23 SS.seek 25.00ml @08:26:20.37 Vacuum too high -- waiting up to 40s @08:26:20.62 Intake @55.1psia, vacuum from 20.0 to 24.0psi @08:26:20.63 SS.reconfigure forSampling @08:26:20.67 SS.seek 25.00ml @08:26:21.43 Vacuum too high -- waiting up to 40s @08:26:21.69 Intake @55.0psia, vacuum from 20.0 to 24.0psi @08:26:21.70 SS.reconfigure forSampling @08:26:21.74 SS.seek 25.00ml @08:26:22.65 Vacuum too high -- waiting up to 40s @08:26:23.11 Intake @55.4psia, vacuum from 20.0 to 24.0psi @08:26:23.12 SS.reconfigure forSampling @08:26:23.16 SS.seek 25.00ml @08:26:24.28 Vacuum too high -- waiting up to 40s @08:26:24.54 Intake @56.4psia, vacuum from 20.0 to 24.0psi @08:26:24.55 SS.reconfigure forSampling @08:26:24.58 SS.seek 25.00ml @08:26:25.31 Vacuum too high -- waiting up to 40s @08:26:25.57 Intake @56.9psia, vacuum from 20.0 to 24.0psi @08:26:25.58 SS.reconfigure forSampling @08:26:25.61 SS.seek 25.00ml @08:26:26.47 Vacuum too high -- waiting up to 40s @08:26:26.73 Intake @56.3psia, vacuum from 20.0 to 24.0psi @08:26:26.74 SS.reconfigure forSampling @08:26:26.77 SS.seek 25.00ml @08:26:28.84 Vacuum too high -- waiting up to 40s @08:26:29.10 Intake @55.6psia, vacuum from 20.0 to 24.0psi @08:26:29.11 SS.reconfigure forSampling @08:26:29.14 SS.seek 25.00ml @08:26:29.93 Vacuum too high -- waiting up to 40s @08:26:30.19 Intake @55.8psia, vacuum from 20.0 to 24.0psi @08:26:30.20 SS.reconfigure forSampling @08:26:30.24 SS.seek 25.00ml @08:26:31.00 Vacuum too high -- waiting up to 40s @08:26:31.26 Intake @56.0psia, vacuum from 20.0 to 24.0psi @08:26:31.27 SS.reconfigure forSampling @08:26:31.30 SS.seek 25.00ml @08:26:32.03 Vacuum too high -- waiting up to 40s @08:26:32.29 Intake @55.9psia, vacuum from 20.0 to 24.0psi @08:26:32.30 SS.reconfigure forSampling @08:26:32.33 SS.seek 25.00ml @08:26:33.09 Vacuum too high -- waiting up to 40s @08:26:33.57 Intake @56.0psia, vacuum from 20.0 to 24.0psi @08:26:33.58 SS.reconfigure forSampling @08:26:33.61 SS.seek 25.00ml @08:26:34.50 Vacuum too high -- waiting up to 40s @08:26:34.76 Intake @55.6psia, vacuum from 20.0 to 24.0psi @08:26:34.77 SS.reconfigure forSampling @08:26:34.80 SS.seek 25.00ml @08:26:35.65 Vacuum too high -- waiting up to 40s @08:26:36.12 Intake @55.5psia, vacuum from 20.0 to 24.0psi @08:26:36.13 SS.reconfigure forSampling @08:26:36.16 SS.seek 25.00ml @08:26:37.43 Vacuum too high -- waiting up to 40s @08:26:37.69 Intake @55.2psia, vacuum from 20.0 to 24.0psi @08:26:37.70 SS.reconfigure forSampling @08:26:37.74 SS.seek 25.00ml @08:26:38.46 Vacuum too high -- waiting up to 40s @08:26:38.93 Intake @55.4psia, vacuum from 20.0 to 24.0psi @08:26:38.94 SS.reconfigure forSampling @08:26:38.97 SS.seek 25.00ml @08:26:40.06 Vacuum too high -- waiting up to 40s @08:26:40.33 Intake @54.9psia, vacuum from 20.0 to 24.0psi @08:26:40.34 SS.reconfigure forSampling @08:26:40.37 SS.seek 25.00ml @08:26:41.37 Vacuum too high -- waiting up to 40s @08:26:41.63 Intake @55.2psia, vacuum from 20.0 to 24.0psi @08:26:41.64 SS.reconfigure forSampling @08:26:41.67 SS.seek 25.00ml @08:26:42.56 Vacuum too high -- waiting up to 40s @08:26:43.04 Intake @56.6psia, vacuum from 20.0 to 24.0psi @08:26:43.05 SS.reconfigure forSampling @08:26:43.08 SS.seek 25.00ml @08:26:44.46 Vacuum too high -- waiting up to 40s @08:26:44.94 Intake @56.2psia, vacuum from 20.0 to 24.0psi @08:26:44.95 SS.reconfigure forSampling @08:26:45.91 SS.seek 25.00ml @08:26:47.38 Vacuum too high -- waiting up to 40s @08:26:47.64 Intake @55.6psia, vacuum from 20.0 to 24.0psi @08:26:47.65 SS.reconfigure forSampling @08:26:47.68 SS.seek 25.00ml @08:26:48.47 Vacuum too high -- waiting up to 40s @08:26:48.72 Intake @55.6psia, vacuum from 20.0 to 24.0psi @08:26:48.73 SS.reconfigure forSampling @08:26:48.77 SS.seek 25.00ml @08:26:49.54 Vacuum too high -- waiting up to 40s @08:26:49.80 SS.seek 25.00ml @08:26:50.47 Vacuum too high -- waiting up to 40s @08:26:50.94 Intake @55.6psia, vacuum from 20.0 to 24.0psi @08:26:50.95 SS.reconfigure forSampling @08:26:50.98 SS.seek 25.00ml @08:26:52.13 Vacuum too high -- waiting up to 40s @08:26:52.39 Intake @55.4psia, vacuum from 20.0 to 24.0psi @08:26:52.40 SS.reconfigure forSampling @08:26:52.43 SS.seek 25.00ml @08:26:53.19 Vacuum too high -- waiting up to 40s @08:26:53.45 Intake @55.2psia, vacuum from 20.0 to 24.0psi @08:26:53.46 SS.reconfigure forSampling @08:26:53.50 SS.seek 25.00ml @08:26:54.32 Vacuum too high -- waiting up to 40s @08:26:54.57 Intake @55.2psia, vacuum from 20.0 to 24.0psi @08:26:54.58 SS.reconfigure forSampling @08:26:54.62 SS.seek 25.00ml @08:26:55.35 Vacuum too high -- waiting up to 40s @08:26:55.61 Intake @55.1psia, vacuum from 20.0 to 24.0psi @08:26:55.62 SS.reconfigure forSampling @08:26:55.65 SS.seek 25.00ml @08:26:56.47 Vacuum too high -- waiting up to 40s @08:26:56.73 Intake @55.0psia, vacuum from 20.0 to 24.0psi @08:26:56.74 SS.reconfigure forSampling @08:26:56.77 SS.seek 25.00ml @08:26:57.44 Vacuum too high -- waiting up to 40s @08:26:57.90 Intake @55.2psia, vacuum from 20.0 to 24.0psi @08:26:58.52 SS.reconfigure forSampling @08:26:58.55 SS.seek 25.00ml @08:26:59.14 Stroke completed -- waiting for pressure to equalize ... @08:27:01.54 Intake.select :close @08:27:01.68 SSV.dial :exhaust @08:27:02.47 Exhaust.select :open @08:27:02.57 SS.reconfigure SSexhale @08:27:02.61 SS.seek 0.00ml @08:27:11.81 Exhaust.select :close @08:27:11.90 SSV.dial CBV @08:27:11.92
CBV.dial SSV @08:27:12.70 Allowing 185s for 25.0ml of remaining 800.0ml @08:27:12.71 Intake.select :open @08:27:14.84 Intake @55.1psia, vacuum from 20.0 to 24.0psi @08:27:14.85 SS.reconfigure forSampling @08:27:14.89 SS.seek 25.00ml @08:27:16.66 Vacuum too high -- waiting up to 40s @08:27:16.92 Intake @55.0psia, vacuum from 20.0 to 24.0psi @08:27:16.93 SS.reconfigure forSampling @08:27:16.96 SS.seek 25.00ml @08:27:17.85 Vacuum too high -- waiting up to 40s @08:27:18.11 Intake @55.3psia, vacuum from 20.0 to 24.0psi @08:27:18.12 SS.reconfigure forSampling @08:27:18.15 SS.seek 25.00ml @08:27:18.94 Vacuum too high -- waiting up to 40s @08:27:19.21 Intake @56.1psia, vacuum from 20.0 to 24.0psi @08:27:19.22 SS.reconfigure forSampling @08:27:19.25 SS.seek 25.00ml @08:27:20.19 Vacuum too high -- waiting up to 40s @08:27:20.67 Intake @56.7psia, vacuum from 20.0 to 24.0psi @08:27:20.68 SS.reconfigure forSampling @08:27:20.71 SS.seek 25.00ml @08:27:21.94 Vacuum too high -- waiting up to 40s @08:27:22.20 Intake @56.2psia, vacuum from 20.0 to 24.0psi @08:27:22.21 SS.reconfigure forSampling @08:27:22.24 SS.seek 25.00ml @08:27:22.88 Vacuum too high -- waiting up to 40s @08:27:23.14 Intake @55.9psia, vacuum from 20.0 to 24.0psi @08:27:23.15 SS.reconfigure forSampling @08:27:23.18 SS.seek 25.00ml @08:27:24.07 Vacuum too high -- waiting up to 40s @08:27:24.55 Intake @55.5psia, vacuum from 20.0 to 24.0psi @08:27:24.56 SS.reconfigure forSampling @08:27:24.59 SS.seek 25.00ml @08:27:25.69 Vacuum too high -- waiting up to 40s @08:27:26.16 Intake @55.6psia, vacuum from 20.0 to 24.0psi @08:27:26.17 SS.reconfigure forSampling @08:27:26.20 SS.seek 25.00ml @08:27:27.35 Vacuum too high -- waiting up to 40s @08:27:27.82 Intake @55.5psia, vacuum from 20.0 to 24.0psi @08:27:27.83 SS.reconfigure forSampling @08:27:27.87 SS.seek 25.00ml @08:27:29.00 Vacuum too high -- waiting up to 40s @08:27:29.47 Intake @55.3psia, vacuum from 20.0 to 24.0psi @08:27:29.48 SS.reconfigure forSampling @08:27:29.52 SS.seek 25.00ml @08:27:30.60 Vacuum too high -- waiting up to 40s @08:27:30.86 Intake @55.5psia, vacuum from 20.0 to 24.0psi @08:27:30.87 SS.reconfigure forSampling @08:27:30.90 SS.seek 25.00ml @08:27:31.63 Vacuum too high -- waiting up to 40s @08:27:32.10 Intake @55.5psia, vacuum from 20.0 to 24.0psi @08:27:32.11 SS.reconfigure forSampling @08:27:32.14 SS.seek 25.00ml @08:27:33.32 Vacuum too high -- waiting up to 40s @08:27:33.79 Intake @55.6psia, vacuum from 20.0 to 24.0psi @08:27:33.80 SS.reconfigure forSampling @08:27:33.83 SS.seek 25.00ml @08:27:34.85 Vacuum too high -- waiting up to 40s @08:27:35.11 Intake @55.7psia, vacuum from 20.0 to 24.0psi @08:27:35.12 SS.reconfigure forSampling @08:27:35.15 SS.seek 25.00ml @08:27:35.88 Vacuum too high -- waiting up to 40s @08:27:36.13 Intake @55.6psia, vacuum from 20.0 to 24.0psi @08:27:36.14 SS.reconfigure forSampling @08:27:36.18 SS.seek 25.00ml @08:27:36.97 Vacuum too high -- waiting up to 40s @08:27:37.44 Intake @56.3psia, vacuum from 20.0 to 24.0psi @08:27:37.45 SS.reconfigure forSampling @08:27:37.48 SS.seek 25.00ml @08:27:38.60 Vacuum too high -- waiting up to 40s @08:27:39.06 Intake @56.8psia, vacuum from 20.0 to 24.0psi @08:27:39.07 SS.reconfigure forSampling @08:27:39.11 SS.seek 25.00ml @08:27:39.94 Vacuum too high -- waiting up to 40s @08:27:40.20 Intake @56.0psia, vacuum from 20.0 to 24.0psi @08:27:40.21 SS.reconfigure forSampling @08:27:40.86 SS.seek 25.00ml @08:27:42.19 Vacuum too high -- waiting up to 40s @08:27:42.45 Intake @55.2psia, vacuum from 20.0 to 24.0psi @08:27:42.46 SS.reconfigure forSampling @08:27:42.50 SS.seek 25.00ml @08:27:43.28 Vacuum too high -- waiting up to 40s @08:27:43.77 Intake @55.4psia, vacuum from 20.0 to 24.0psi @08:27:43.78 SS.reconfigure forSampling @08:27:43.82 SS.seek 25.00ml @08:27:45.00 Vacuum too high -- waiting up to 40s @08:27:45.28 Intake @55.2psia, vacuum from 20.0 to 24.0psi @08:27:45.29 SS.reconfigure forSampling @08:27:45.32 SS.seek 25.00ml @08:27:46.22 Vacuum too high -- waiting up to 40s @08:27:46.69 Intake @55.0psia, vacuum from 20.0 to 24.0psi @08:27:46.70 SS.reconfigure forSampling @08:27:46.73 SS.seek 25.00ml @08:27:47.81 Vacuum too high -- waiting up to 40s @08:27:48.07 Intake @55.2psia, vacuum from 20.0 to 24.0psi @08:27:48.08 SS.reconfigure forSampling @08:27:48.11 SS.seek 25.00ml @08:27:48.91 Vacuum too high -- waiting up to 40s @08:27:49.17 Intake @55.5psia, vacuum from 20.0 to 24.0psi @08:27:49.18 SS.reconfigure forSampling @08:27:49.21 SS.seek 25.00ml @08:27:50.03 Vacuum too high -- waiting up to 40s @08:27:50.29 Intake @55.9psia, vacuum from 20.0 to 24.0psi @08:27:50.30 SS.reconfigure forSampling @08:27:50.33 SS.seek 25.00ml @08:27:50.94 Vacuum too high -- waiting up to 40s @08:27:51.21 SS.seek 25.00ml @08:27:51.75 Vacuum too high -- waiting up to 40s @08:27:52.01 Intake @55.7psia, vacuum from 20.0 to 24.0psi @08:27:52.02 SS.reconfigure forSampling @08:27:52.05 SS.seek 25.00ml @08:27:52.88 Vacuum too high -- waiting up to 40s @08:27:53.37 SS.seek 25.00ml @08:27:54.38 Vacuum too high -- waiting up to 40s @08:27:54.64 Intake @55.6psia, vacuum from 20.0 to 24.0psi @08:27:54.65 SS.reconfigure forSampling @08:27:54.68 SS.seek 25.00ml @08:27:55.47 Vacuum too high -- waiting up to 40s @08:27:55.94 Intake @55.5psia, vacuum from 20.0 to 24.0psi @08:27:55.95 SS.reconfigure forSampling @08:27:55.98 SS.seek 25.00ml @08:27:57.10 Vacuum too high -- waiting up to 40s @08:27:57.57 Intake @55.5psia, vacuum from 20.0 to 24.0psi @08:27:57.58 SS.reconfigure forSampling @08:27:57.61 SS.seek 25.00ml @08:27:58.78 Vacuum too high -- waiting up to 40s @08:27:59.26 Intake @56.1psia, vacuum from 20.0 to 24.0psi @08:27:59.27 SS.reconfigure forSampling @08:27:59.30 SS.seek 25.00ml @08:28:00.19 Vacuum too high -- waiting up to 40s @08:28:00.45 Intake @56.2psia, vacuum from 20.0 to 24.0psi @08:28:00.46 SS.reconfigure forSampling @08:28:00.49 SS.seek 25.00ml @08:28:01.35 Vacuum too high -- waiting up to 40s @08:28:01.82 SS.seek 25.00ml @08:28:02.69 Vacuum too high -- waiting up to 40s @08:28:02.94 Intake @56.1psia, vacuum from 20.0 to 24.0psi @08:28:02.95 SS.reconfigure forSampling @08:28:02.99 SS.seek 25.00ml @08:28:03.85 Vacuum too high -- waiting up to 40s @08:28:04.10 Intake @56.0psia, vacuum from 20.0 to 24.0psi @08:28:04.11 SS.reconfigure forSampling @08:28:04.15 SS.seek 25.00ml @08:28:04.88 Vacuum too high -- waiting up to 40s @08:28:05.35 Intake @55.8psia, vacuum from 20.0 to 24.0psi @08:28:05.36 SS.reconfigure forSampling @08:28:05.39 SS.seek 25.00ml @08:28:06.35 Vacuum too high -- waiting up to 40s @08:28:06.61 Intake @55.7psia, vacuum from 20.0 to 24.0psi @08:28:06.62 SS.reconfigure forSampling @08:28:06.65 SS.seek 25.00ml @08:28:07.44 Vacuum too high -- waiting up to 40s @08:28:07.69 Intake @55.8psia, vacuum from 20.0 to 24.0psi @08:28:07.70 SS.reconfigure forSampling @08:28:07.74 SS.seek 25.00ml @08:28:08.44 Vacuum too high -- waiting up to 40s @08:28:08.91 Intake @55.8psia, vacuum from 20.0 to 24.0psi @08:28:08.92 SS.reconfigure forSampling @08:28:08.95 SS.seek 25.00ml @08:28:09.97 Vacuum too high -- waiting up to 40s @08:28:10.87 Intake @56.2psia, vacuum from 20.0 to 24.0psi @08:28:10.88 SS.reconfigure forSampling @08:28:10.91 SS.seek 25.00ml @08:28:12.38 Vacuum too high -- waiting up to 40s @08:28:12.86 Intake @56.5psia, vacuum from 20.0 to 24.0psi @08:28:12.87 SS.reconfigure forSampling @08:28:12.90 SS.seek 25.00ml @08:28:13.78 Vacuum too high -- waiting up to 40s @08:28:14.04 Intake @55.9psia, vacuum from 20.0 to 24.0psi @08:28:14.05 SS.reconfigure forSampling @08:28:14.09 SS.seek 25.00ml @08:28:14.88 Vacuum too high -- waiting up to 40s @08:28:15.15 Intake @55.8psia, vacuum from 20.0 to 24.0psi @08:28:15.16 SS.reconfigure forSampling @08:28:15.19 SS.seek 25.00ml @08:28:15.97 Vacuum too high -- waiting up to 40s @08:28:16.45 SS.seek 25.00ml @08:28:17.38 Vacuum too high -- waiting up to 40s @08:28:17.64 Intake @55.9psia, vacuum from 20.0 to 24.0psi @08:28:17.65 SS.reconfigure forSampling @08:28:17.69 SS.seek 25.00ml @08:28:18.50 Vacuum too high -- waiting up to 40s @08:28:18.97 Intake @55.6psia, vacuum from 20.0 to 24.0psi @08:28:18.98 SS.reconfigure forSampling @08:28:19.01 SS.seek 25.00ml @08:28:20.03 Vacuum too high -- waiting up to 40s @08:28:20.51 Intake @55.8psia, vacuum from 20.0 to 24.0psi @08:28:20.52 SS.reconfigure forSampling @08:28:20.55 SS.seek 25.00ml @08:28:21.59 Vacuum too high -- waiting up to 40s @08:28:21.85 Intake @55.8psia, vacuum from 20.0 to 24.0psi @08:28:21.86 SS.reconfigure forSampling @08:28:21.89 SS.seek 25.00ml @08:28:22.66 Vacuum too high -- waiting up to 40s @08:28:22.93 Intake @56.1psia, vacuum from 20.0 to 24.0psi @08:28:22.94 SS.reconfigure forSampling @08:28:22.97 SS.seek 25.00ml @08:28:23.63 Vacuum too high -- waiting up to 40s @08:28:23.89 Intake @55.9psia, vacuum from 20.0 to 24.0psi @08:28:23.90 SS.reconfigure forSampling @08:28:23.93 SS.seek 25.00ml @08:28:24.56 Vacuum too high -- waiting up to 40s @08:28:24.82 Intake @55.8psia, vacuum from 20.0 to 24.0psi @08:28:24.83 SS.reconfigure forSampling @08:28:24.87 SS.seek 25.00ml @08:28:25.56 Vacuum too high -- waiting up to 40s @08:28:25.82 Intake @55.6psia, vacuum from 20.0 to 24.0psi @08:28:25.83 SS.reconfigure forSampling @08:28:25.87 SS.seek 25.00ml @08:28:26.59 Vacuum too high -- waiting up to 40s @08:28:26.85 Intake @55.7psia, vacuum from 20.0 to 24.0psi @08:28:26.86 SS.reconfigure forSampling @08:28:26.89 SS.seek 25.00ml @08:28:27.63 Vacuum too high -- waiting up to 40s @08:28:27.89 Intake @55.9psia, vacuum from 20.0 to 24.0psi @08:28:27.90 SS.reconfigure forSampling @08:28:27.93 SS.seek 25.00ml @08:28:28.56 Vacuum too high -- waiting up to 40s @08:28:29.03 Intake @57.2psia, vacuum from 20.0 to 24.0psi @08:28:29.04 SS.reconfigure forSampling @08:28:29.07 SS.seek 25.00ml @08:28:30.05 Vacuum too high -- waiting up to 40s @08:28:30.52 Intake @56.6psia, vacuum from 20.0 to 24.0psi @08:28:30.53 SS.reconfigure forSampling @08:28:30.57 SS.seek 25.00ml @08:28:31.47 Vacuum too high -- waiting up to 40s @08:28:31.73 Intake @56.1psia, vacuum from 20.0 to 24.0psi @08:28:31.74 SS.reconfigure forSampling @08:28:31.77 SS.seek 25.00ml @08:28:32.47 Vacuum too high -- waiting up to 40s @08:28:32.74 Intake @55.9psia, vacuum from 20.0 to 24.0psi SS.reconfigure forSampling @08:28:32.78 SS.seek 25.00ml @08:28:33.50 Vacuum too high -- waiting up to 40s @08:28:33.97 Intake @55.6psia, vacuum from 20.0 to 24.0psi @08:28:33.98 SS.reconfigure forSampling @08:28:34.01 SS.seek 25.00ml @08:28:35.00 Vacuum too high -- waiting up to 40s @08:28:35.48 Intake @55.6psia, vacuum from 20.0 to 24.0psi @08:28:35.49 SS.reconfigure forSampling @08:28:35.61 SS.seek 25.00ml @08:28:36.59 Vacuum too high -- waiting up to 40s @08:28:37.06 Intake @55.5psia, vacuum from 20.0 to 24.0psi @08:28:37.07 SS.reconfigure forSampling @08:28:37.10 SS.seek 25.00ml @08:28:37.91 Vacuum too high -- waiting up to 40s @08:28:38.78 Intake @55.5psia, vacuum from 20.0 to 24.0psi @08:28:38.80 SS.reconfigure forSampling @08:28:38.83 SS.seek 25.00ml @08:28:40.00 Vacuum too high -- waiting up to 40s @08:28:40.49 Intake @55.6psia, vacuum from 20.0 to 24.0psi @08:28:40.50 SS.reconfigure forSampling @08:28:40.53 SS.seek 25.00ml @08:28:41.56 Vacuum too high -- waiting up to 40s @08:28:42.05 Intake @56.1psia, vacuum from 20.0 to 24.0psi @08:28:42.06 SS.reconfigure forSampling @08:28:42.09 SS.seek 25.00ml @08:28:43.16 Vacuum too high -- waiting up to 40s @08:28:43.63 Intake @56.7psia, vacuum from 20.0 to 24.0psi @08:28:43.64 SS.reconfigure forSampling @08:28:43.68 SS.seek 25.00ml @08:28:44.59 Vacuum too high -- waiting up to 40s @08:28:44.85 Intake @56.4psia, vacuum from 20.0 to 24.0psi @08:28:44.86 SS.reconfigure forSampling @08:28:44.89 SS.seek 25.00ml @08:28:45.50 Vacuum too high -- waiting up to 40s @08:28:45.76 Intake @56.6psia, vacuum from 20.0 to 24.0psi @08:28:45.77 SS.reconfigure forSampling @08:28:45.81 SS.seek 25.00ml @08:28:46.53 Vacuum too high -- waiting up to 40s @08:28:47.01 Intake @56.4psia, vacuum from 20.0 to 24.0psi @08:28:47.02 SS.reconfigure forSampling @08:28:47.05 SS.seek 25.00ml @08:28:48.03 Vacuum too high -- waiting up to 40s @08:28:48.51 Intake @56.1psia, vacuum from 20.0 to 24.0psi @08:28:48.52 SS.reconfigure forSampling @08:28:48.55 SS.seek 25.00ml @08:28:49.53 Vacuum too high -- waiting up to 40s @08:28:50.01 Intake @56.3psia, vacuum from 20.0 to 24.0psi @08:28:50.02 SS.reconfigure forSampling @08:28:50.06 SS.seek 25.00ml @08:28:51.06 Vacuum too high -- waiting up to 40s @08:28:51.53 Intake @56.0psia, vacuum from 20.0 to 24.0psi @08:28:51.54 SS.reconfigure forSampling @08:28:51.57 SS.seek 25.00ml @08:28:52.53 Vacuum too high -- waiting up to 40s @08:28:52.79 Intake @55.7psia, vacuum from 20.0 to 24.0psi @08:28:52.80 SS.reconfigure forSampling @08:28:52.83 SS.seek 25.00ml @08:28:53.44 Vacuum too high -- waiting up to 40s @08:28:53.70 Intake @55.6psia, vacuum from 20.0 to 24.0psi @08:28:53.71 SS.reconfigure forSampling @08:28:53.74 SS.seek 25.00ml @08:28:54.50 Vacuum too high -- waiting up to 40s @08:28:54.97 Intake @55.5psia, vacuum from 20.0 to 24.0psi @08:28:54.98 SS.reconfigure forSampling @08:28:55.01 SS.seek 25.00ml @08:28:55.91 Vacuum too high -- waiting up to 40s @08:28:56.38 Intake @55.3psia, vacuum from 20.0 to 24.0psi @08:28:56.39 SS.reconfigure forSampling @08:28:56.42 SS.seek 25.00ml @08:28:57.31 Vacuum too high -- waiting up to 40s @08:28:57.78 Intake @55.5psia, vacuum from 20.0 to 24.0psi @08:28:57.79 SS.reconfigure forSampling @08:28:57.82 SS.seek 25.00ml @08:28:58.78 Vacuum too high -- waiting up to 40s @08:28:59.04 Intake @56.1psia, vacuum from 20.0 to 24.0psi @08:28:59.05 SS.reconfigure forSampling @08:28:59.08 SS.seek 25.00ml @08:28:59.72 Vacuum too high -- waiting up to 40s @08:28:59.97 Intake @56.0psia, vacuum from 20.0 to 24.0psi @08:28:59.99 SS.reconfigure forSampling @08:29:00.02 SS.seek 25.00ml @08:29:00.75 Vacuum too high -- waiting up to 40s @08:29:01.22 Intake @55.9psia, vacuum from 20.0 to 24.0psi @08:29:01.23 SS.reconfigure forSampling @08:29:01.26 SS.seek 25.00ml @08:29:02.28 Vacuum too high -- waiting up to 40s @08:29:02.53 Intake @55.7psia, vacuum from 20.0 to 24.0psi @08:29:02.54 SS.reconfigure forSampling @08:29:02.58 SS.seek 25.00ml @08:29:03.34 Vacuum too high -- waiting up to 40s @08:29:03.81 Intake @55.6psia, vacuum from 20.0 to 24.0psi @08:29:03.82 SS.reconfigure forSampling @08:29:03.85 SS.seek 25.00ml @08:29:04.75 Vacuum too high -- waiting up to 40s @08:29:05.22 Intake @55.5psia, vacuum from 20.0 to 24.0psi @08:29:05.23 SS.reconfigure forSampling @08:29:05.26 SS.seek 25.00ml @08:29:06.15 Vacuum too high -- waiting up to 40s @08:29:07.05 Intake @55.2psia, vacuum from 20.0 to 24.0psi @08:29:07.06 SS.reconfigure forSampling @08:29:07.09 SS.seek 25.00ml @08:29:08.37 Vacuum too high -- waiting up to 40s @08:29:08.85 Intake @55.5psia, vacuum from 20.0 to 24.0psi @08:29:08.86 SS.reconfigure forSampling @08:29:08.89 SS.seek 25.00ml @08:29:09.78 Vacuum too high -- waiting up to 40s @08:29:10.26 Intake @56.2psia, vacuum from 20.0 to 24.0psi @08:29:10.27 SS.reconfigure forSampling @08:29:10.30 SS.seek 25.00ml @08:29:11.25 Vacuum too high -- waiting up to 40s @08:29:11.73 Intake @56.2psia, vacuum from 20.0 to 24.0psi @08:29:11.74 SS.reconfigure forSampling @08:29:11.77 SS.seek 25.00ml @08:29:12.87 Vacuum too high -- waiting up to 40s @08:29:13.34 Intake @55.8psia, vacuum from 20.0 to 24.0psi @08:29:13.35 SS.reconfigure forSampling @08:29:13.38 SS.seek 25.00ml @08:29:14.31 Vacuum too high -- waiting up to 40s @08:29:14.57 Intake @55.5psia, vacuum from 20.0 to 24.0psi @08:29:14.58 SS.reconfigure forSampling @08:29:14.61 SS.seek 25.00ml @08:29:15.19 Vacuum too high -- waiting up to 40s @08:29:15.45 Intake @55.6psia, vacuum from 20.0 to 24.0psi @08:29:15.46 SS.reconfigure forSampling @08:29:15.50 SS.seek 25.00ml @08:29:16.22 Vacuum too high -- waiting up to 40s @08:29:16.48 Intake @55.1psia, vacuum from 20.0 to 24.0psi @08:29:16.50 SS.reconfigure forSampling @08:29:16.53 SS.seek 25.00ml @08:29:17.15 Vacuum too high -- waiting up to 40s @08:29:17.62 Intake @54.9psia, vacuum from 20.0 to 24.0psi @08:29:17.63 SS.reconfigure forSampling @08:29:17.66 SS.seek 25.00ml @08:29:18.56 Vacuum too high -- waiting up to 40s @08:29:19.04 SS.seek 25.00ml @08:29:19.94 Vacuum too high -- waiting up to 40s @08:29:20.43 Intake @55.4psia, vacuum from 20.0 to 24.0psi @08:29:20.44 SS.reconfigure forSampling @08:29:20.47 SS.seek 25.00ml @08:29:21.62 Vacuum too high -- waiting up to 40s @08:29:22.10 Intake @55.5psia, vacuum from 20.0 to 24.0psi @08:29:22.11 SS.reconfigure forSampling @08:29:22.14 SS.seek 25.00ml @08:29:23.03 Vacuum too high -- waiting up to 40s @08:29:23.29 Intake @55.8psia, vacuum from 20.0 to 24.0psi @08:29:23.30 SS.reconfigure forSampling @08:29:23.33 SS.seek 25.00ml @08:29:24.09 Vacuum too high -- waiting up to 40s @08:29:24.56 Intake @56.4psia, vacuum from 20.0 to 24.0psi @08:29:24.57 SS.reconfigure forSampling @08:29:24.61 SS.seek 25.00ml @08:29:25.53 Vacuum too high -- waiting up to 40s @08:29:26.00 Intake @56.2psia, vacuum from 20.0 to 24.0psi @08:29:26.01 SS.reconfigure forSampling @08:29:26.04 SS.seek 25.00ml @08:29:26.90 Vacuum too high -- waiting up to 40s @08:29:27.36 Intake @56.2psia, vacuum from 20.0 to 24.0psi @08:29:27.37 SS.reconfigure forSampling @08:29:27.41 SS.seek 25.00ml @08:29:28.37 Vacuum too high -- waiting up to 40s @08:29:28.84 Intake @56.2psia, vacuum from 20.0 to 24.0psi @08:29:28.85 SS.reconfigure forSampling @08:29:28.88 SS.seek 25.00ml @08:29:29.75 Vacuum too high -- waiting up to 40s @08:29:30.02 Intake @56.0psia, vacuum from 20.0 to 24.0psi @08:29:30.03 SS.reconfigure forSampling @08:29:30.06 SS.seek 25.00ml @08:29:30.68 Vacuum too high -- waiting up to 40s @08:29:31.14 Intake @56.2psia, vacuum from 20.0 to 24.0psi @08:29:31.15 SS.reconfigure forSampling @08:29:31.19 SS.seek 25.00ml @08:29:31.84 Vacuum too high -- waiting up to 40s @08:29:32.31 Intake @56.1psia, vacuum from 20.0 to 24.0psi @08:29:32.32 SS.reconfigure forSampling @08:29:32.35 SS.seek 25.00ml @08:29:33.12 Vacuum too high -- waiting up to 40s @08:29:33.58 Intake @56.0psia, vacuum from 20.0 to 24.0psi @08:29:33.59 SS.reconfigure forSampling @08:29:33.63 SS.seek 25.00ml @08:29:35.50 Vacuum too high -- waiting up to 40s @08:29:35.99 Intake @55.0psia, vacuum from 20.0 to 24.0psi @08:29:36.00 SS.reconfigure forSampling @08:29:36.03 SS.seek 25.00ml @08:29:37.00 Vacuum too high -- waiting up to 40s @08:29:37.48 Intake @54.7psia, vacuum from 20.0 to 24.0psi @08:29:37.49 SS.reconfigure forSampling @08:29:37.52 SS.seek 25.00ml @08:29:38.56 Vacuum too high -- waiting up to 40s @08:29:39.05 Intake @54.8psia, vacuum from 20.0 to 24.0psi @08:29:39.06 SS.reconfigure forSampling @08:29:39.10 SS.seek 25.00ml @08:29:40.03 Vacuum too high -- waiting up to 40s @08:29:40.50 Intake @54.9psia, vacuum from 20.0 to 24.0psi @08:29:40.51 SS.reconfigure forSampling @08:29:40.54 SS.seek 25.00ml @08:29:41.37 Vacuum too high -- waiting up to 40s @08:29:41.85 Intake @55.1psia, vacuum from 20.0 to 24.0psi @08:29:41.86 SS.reconfigure forSampling @08:29:41.89 SS.seek 25.00ml @08:29:42.78 Vacuum too high -- waiting up to 40s @08:29:43.25 Intake @55.5psia, vacuum from 20.0 to 24.0psi @08:29:43.26 SS.reconfigure forSampling @08:29:43.29 SS.seek 25.00ml @08:29:44.15 Vacuum too high -- waiting up to 40s @08:29:44.61 Intake @55.6psia, vacuum from 20.0 to 24.0psi @08:29:44.62 SS.reconfigure forSampling @08:29:44.66 SS.seek 25.00ml @08:29:45.50 Vacuum too high -- waiting up to 40s @08:29:45.97 Intake @55.4psia, vacuum from 20.0 to 24.0psi @08:29:45.98 SS.reconfigure forSampling @08:29:46.01 SS.seek 25.00ml @08:29:46.96 Vacuum too high -- waiting up to 40s @08:29:47.42 Intake @55.4psia, vacuum from 20.0 to 24.0psi @08:29:47.43 SS.reconfigure forSampling @08:29:47.47 SS.seek 25.00ml @08:29:48.34 Vacuum too high -- waiting up to 40s @08:29:48.80 Intake @55.5psia, vacuum from 20.0 to 24.0psi @08:29:48.81 SS.reconfigure forSampling @08:29:48.85 SS.seek 25.00ml @08:29:49.65 Vacuum too high -- waiting up to 40s @08:29:50.12 Intake @55.6psia, vacuum from 20.0 to 24.0psi @08:29:50.13 SS.reconfigure forSampling @08:29:50.17 SS.seek 25.00ml @08:29:50.93 Vacuum too high -- waiting up to 40s @08:29:51.19 Intake @55.5psia, vacuum from 20.0 to 24.0psi @08:29:51.20 SS.reconfigure forSampling @08:29:51.23 SS.seek 25.00ml @08:29:51.81 Vacuum too high -- waiting up to 40s @08:29:52.28 Intake @55.4psia, vacuum from 20.0 to 24.0psi @08:29:52.29 SS.reconfigure forSampling @08:29:52.32 SS.seek 25.00ml @08:29:53.31 Vacuum too high -- waiting up to 40s @08:29:53.77 Intake @55.4psia, vacuum from 20.0 to 24.0psi @08:29:53.78 SS.reconfigure forSampling @08:29:53.82 SS.seek 25.00ml @08:29:54.68 Vacuum too high -- waiting up to 40s @08:29:55.15 Intake @55.6psia, vacuum from 20.0 to 24.0psi @08:29:55.16 SS.reconfigure forSampling @08:29:55.20 SS.seek 25.00ml @08:29:56.03 Vacuum too high -- waiting up to 40s @08:29:56.49 Intake @55.4psia, vacuum from 20.0 to 24.0psi @08:29:56.50 SS.reconfigure forSampling @08:29:56.54 SS.seek 25.00ml @08:29:57.40 Vacuum too high -- waiting up to 40s @08:29:57.86 Intake @55.4psia, vacuum from 20.0 to 24.0psi @08:29:57.87 SS.reconfigure forSampling @08:29:57.91 SS.seek 25.00ml @08:29:58.78 Vacuum too high -- waiting up to 40s @08:29:59.04 Intake @55.3psia, vacuum from 20.0 to 24.0psi @08:29:59.05 SS.reconfigure forSampling @08:29:59.08 SS.seek 25.00ml @08:29:59.68 Vacuum too high -- waiting up to 40s @08:30:00.15 Intake @55.5psia, vacuum from 20.0 to 24.0psi @08:30:00.16 SS.reconfigure forSampling @08:30:00.19 SS.seek 25.00ml @08:30:01.03 Vacuum too high -- waiting up to 40s @08:30:01.49 Intake @55.7psia, vacuum from 20.0 to 24.0psi @08:30:02.17 SS.reconfigure forSampling @08:30:02.20 SS.seek 25.00ml @08:30:03.46 Vacuum too high -- waiting up to 40s @08:30:03.93 Intake @56.0psia, vacuum from 20.0 to 24.0psi @08:30:03.94 SS.reconfigure forSampling @08:30:03.97 SS.seek 25.00ml @08:30:04.96 Vacuum too high -- waiting up to 40s @08:30:05.44 Intake @56.2psia, vacuum from 20.0 to 24.0psi @08:30:05.45 SS.reconfigure forSampling @08:30:05.48 SS.seek 25.00ml @08:30:06.43 Vacuum too high -- waiting up to 40s @08:30:06.69 Intake @56.1psia, vacuum from 20.0 to 24.0psi @08:30:06.70 SS.reconfigure forSampling @08:30:06.73 SS.seek 25.00ml @08:30:07.28 Vacuum too high -- waiting up to 40s @08:30:07.54 Intake @56.0psia, vacuum from 20.0 to 24.0psi @08:30:07.55 SS.reconfigure forSampling @08:30:07.59 SS.seek 25.00ml @08:30:08.28 Vacuum too high -- waiting up to 40s @08:30:08.75 Intake @55.8psia, vacuum from 20.0 to 24.0psi @08:30:08.76 SS.reconfigure forSampling @08:30:08.79 SS.seek 25.00ml @08:30:09.62 Vacuum too high -- waiting up to 40s @08:30:10.10 Intake @55.7psia, vacuum from 20.0 to 24.0psi @08:30:10.11 SS.reconfigure forSampling @08:30:10.14 SS.seek 25.00ml @08:30:10.81 Vacuum too high -- waiting up to 40s @08:30:11.28 Intake @55.7psia, vacuum from 20.0 to 24.0psi @08:30:11.29 SS.reconfigure forSampling @08:30:11.32 SS.seek 25.00ml @08:30:12.21 Vacuum too high -- waiting up to 40s @08:30:12.69 Intake @55.8psia, vacuum from 20.0 to 24.0psi @08:30:12.70 SS.reconfigure forSampling @08:30:12.73 SS.seek 25.00ml @08:30:13.62 Vacuum too high -- waiting up to 40s @08:30:14.10 Intake @55.5psia, vacuum from 20.0 to 24.0psi @08:30:14.11 SS.reconfigure forSampling @08:30:14.14 SS.seek 25.00ml @08:30:15.15 Vacuum too high -- waiting up to 40s @08:30:15.63 Intake @55.5psia, vacuum from 20.0 to 24.0psi @08:30:15.64 SS.reconfigure forSampling @08:30:15.67 SS.seek 25.00ml @08:30:16.56 Vacuum too high -- waiting up to 40s @08:30:17.04 Intake @55.3psia, vacuum from 20.0 to 24.0psi @08:30:17.05 SS.reconfigure forSampling @08:30:17.08 SS.seek 25.00ml @08:30:17.72 SS.reconfigure SSconfig @08:30:17.75 SS.hold @08:30:17.94 Intake.select :close @08:30:18.08 ShallowSampler::Clogged in MAIN -- progress too slow after 221.5ml SSV.dial :exhaust @08:30:18.86 Exhaust.select :open @08:30:18.96 SS.reconfigure SSexhale @08:30:19.00 SS.seek 0.00ml @08:30:26.88 Exhaust.select :close @08:30:26.98 Sampled 221.5ml @08:30:26.99 collectSample: Complete Evacuating Puck (bubblePt=55.0psi, delay=15.0s) @08:30:27.00 Intake.select :close @08:30:27.05 CSV.dial :waste @08:30:27.09 CS.seek 0.000ml @08:30:27.17
CBV.dial SSV @08:30:27.18 SSV.dial CBV,avoiding => exhaust @08:30:27.22 CSV.dial :air @08:30:28.04 CS.seek 10.000ml @08:30:29.14
CTV.dial :air @08:30:29.92 delay 15 seconds @08:30:29.97 Sleepy.power :sampler,:OFF @08:30:44.92
SSV.dial :spit,avoiding => exhaust @08:30:46.26 CTV.dial CSV,avoiding => intake @08:30:46.31
SS.seek 0.00ml @08:30:46.45 SSV.dial CBV,avoiding => exhaust @08:30:52.69 CBV.dial between CSV and waste2b @08:30:53.61 CTV.dial CSV,avoiding => air @08:30:53.63
CSV.dial CTV @08:30:54.07 CS.reconfigure CSpressurize @08:30:54.10 CS.seek 0.000ml @08:31:17.71 BubblePoint: Intake @70.0psia, Outlet @14.5psia @08:31:17.72 SS.reconfigure SSconfig @08:31:17.76 SS.seek 10.00ml @08:31:23.45 delay 15 seconds @08:31:38.54 Evacuated: Intake @69.5psia, Outlet @-0.1psia @08:31:38.55 SSV.dial :spit,avoiding => exhaust @08:31:39.87 SS.reconfigure SSexhale @08:31:39.90 SS.seek 0.00ml @08:31:45.02 SS.reconfigure SSfast @08:31:45.06 SS.seek 5.00ml @08:31:47.68 SSV.dial CBV,avoiding => exhaust @08:31:48.28
CBV.dial SSV @08:31:49.64 CTV.dial :air,avoiding => intake @08:31:50.94 CSV.dial CTV @08:31:50.96
SS.reconfigure SSconfig @08:31:51.00 SS.seek 10.00ml @08:31:51.96 CSV.dial :air @08:31:52.49 CS.reconfigure CSfast @08:31:52.52 CS.seek 0.000ml @08:31:54.05
SSV.dial :spit,avoiding => exhaust @08:31:55.26 CS.coast @08:31:55.39
SS.reconfigure SSexhale @08:31:55.42 SS.seek 0.00ml @08:32:00.33 SS.reconfigure SSfast @08:32:00.37 SS.seek 5.00ml @08:32:03.10 SSV.dial CBV,avoiding => exhaust @08:32:03.12
CBV.dial SSV @08:32:05.42 SSV.dial :spit,avoiding => exhaust @08:32:06.73 SS.reconfigure SSconfig @08:32:06.76 SS.seek 0.00ml @08:32:09.94 SS.coast @08:32:09.97 Evacuating Puck (bubblePt=55.0psi, delay=15.0s) Intake.select :close @08:32:10.03 CSV.dial :waste @08:32:10.13 SSV.dial CBV,avoiding => exhaust @08:32:10.15
CBV.dial SSV @08:32:10.77 CS.seek 0.000ml @08:32:11.04 CSV.dial :air @08:32:11.48
CTV.dial :air @08:32:11.49 delay 15 seconds @08:32:11.84 CS.seek 10.000ml @08:32:26.49
SSV.dial :spit,avoiding => exhaust @08:32:27.82 CTV.dial CSV,avoiding => intake @08:32:27.84
SS.reconfigure SSexhale @08:32:27.91 SS.seek 0.00ml @08:32:28.40 SSV.dial CBV,avoiding => exhaust @08:32:35.75 CBV.dial between CSV and waste2b @08:32:36.68 CSV.dial between air and CTV @08:32:36.99 CS.seek 7.097ml @08:32:44.40 CTV.dial CSV,avoiding => air @08:32:44.41
CSV.dial CTV @08:32:44.71 CS.reconfigure CSpressurize @08:32:44.74 CS.seek 0.000ml @08:33:03.23 CSV.dial :air @08:33:03.75 CS.reconfigure CSfast @08:33:03.78 CS.seek 10.000ml @08:33:27.39 CSV.dial between air and CTV @08:33:27.78 CS.seek 2.036ml @08:33:46.74 CTV.dial CSV,avoiding => air @08:33:46.75
CSV.dial CTV @08:33:47.05 CS.reconfigure CSpressurize @08:33:47.08 CS.seek 0.000ml @08:33:48.15 BubblePoint: Intake @73.8psia, Outlet @18.5psia @08:33:48.16 SS.reconfigure SSconfig @08:33:48.19 SS.seek 10.00ml @08:33:53.95 delay 15 seconds @08:34:09.05 Evacuated: Intake @73.2psia, Outlet @-0.1psia SSV.dial :spit,avoiding => exhaust @08:34:10.38 SS.reconfigure SSexhale @08:34:10.41 SS.seek 0.00ml @08:34:15.37 SS.reconfigure SSfast @08:34:15.41 SS.seek 5.00ml @08:34:18.27 SSV.dial CBV,avoiding => exhaust @08:34:18.29
CBV.dial SSV @08:34:19.61 CTV.dial :air,avoiding => intake @08:34:20.90 CSV.dial CTV @08:34:20.92
SS.reconfigure SSconfig @08:34:20.96 SS.seek 10.00ml @08:34:21.92 CSV.dial :air @08:34:22.44 CS.reconfigure CSfast @08:34:22.47 CS.seek 0.000ml @08:34:24.37
SSV.dial :spit,avoiding => exhaust @08:34:25.71 SS.reconfigure SSexhale @08:34:25.74 SS.seek 0.00ml @08:34:27.41 CS.coast @08:34:30.55
SS.reconfigure SSfast @08:34:30.58 SS.seek 5.00ml @08:34:33.42 SSV.dial CBV,avoiding => exhaust @08:34:33.43
CBV.dial SSV @08:34:35.76 SSV.dial :spit,avoiding => exhaust @08:34:37.08 SS.reconfigure SSconfig @08:34:37.11 SS.seek 0.00ml @08:34:40.26 SS.coast @08:34:40.29 Lysing: Adding 1.5ml lysis CBV.dial between CSV and waste1,avoiding => DBV @08:34:41.78 CTV.dial CSV @08:34:43.04 CSV.dial CSR @08:34:43.82 CSR.select :lysis @08:34:43.92 CS.reconfigure CSconfig @08:34:43.96 CS.seek 1.000ml @08:34:47.26 delay 5 seconds @08:34:52.27 CSR.select :air @08:34:52.41 CS.seek 6.000ml @08:35:05.91 delay 2 seconds @08:35:07.92 CSV.dial :waste1 @08:35:09.75 CS.reconfigure CSfast @08:35:09.78 CS.seek empty @08:35:24.25 CSV.dial CSR @08:35:25.52 CSR.select :lysis @08:35:25.62 CS.reconfigure CSconfig @08:35:25.66 CS.seek 1.500ml @08:35:30.29 delay 5 seconds @08:35:35.31 CSR.select :air @08:35:35.45 CS.seek 4.500ml @08:35:43.82 delay 2 seconds @08:35:45.83 CSV.dial CTV @08:35:46.34 CS.seek 1.275ml @08:35:55.26 Heating sh1 puck from tube 3 in Collection Clamp to 85.0C for 5:00 @08:35:55.27 CH.seek 85.0C,8:00 @08:38:43.84 CH.hold 5:00,85.0C @08:43:43.94 Collection Puck Heater at 84.9C after lysing @08:43:44.66 CS.seek 0.000ml @08:43:48.77 CTV.dial between spare and air,avoiding => intake @08:43:49.77 CSV.dial :air @08:43:50.22 CS.seek 5.000ml @08:44:03.74 CSV.dial CTV @08:44:04.26 delay 2 seconds @08:44:06.27 CSV.dial :air @08:44:06.79 delay 2 seconds @08:44:08.80 CSV.dial CTV @08:44:09.32 delay 2 seconds @08:44:11.33 CSV.dial :air @08:44:11.85 delay 2 seconds @08:44:13.86 CS.seek 0.000ml @08:44:27.43 Recovering Lysate into Collection syringe @08:44:27.44 CSV.dial CBV @08:44:28.43 CBV.dial CSV @08:44:28.77 CS.seek 4.999ml @08:44:42.24 delay 5 seconds @08:44:47.25 CTV.dial :air @08:44:47.73 CS.seek 6.999ml @08:44:53.58 delay 5 seconds @08:44:58.59 CSV.dial :air2 @08:44:59.01 CS.seek 10.000ml @08:45:07.43 CSV.dial between air and CTV,avoiding => CBV @08:45:08.56 Lysing Complete @08:45:08.61 Storing sh1 puck in Collection Clamp to tube 2 @08:45:08.73 SC.reconfigure SCtight @08:45:08.76 SC.seek 2 @08:45:10.99 SC.reconfigure SCloose @08:45:11.16 Elbow.reconfigure TightElbow @08:45:11.19 Elbow.seek 2 @08:45:12.78 Forearm.reconfigure TightForearm @08:45:12.81 Forearm.seek 2 + 800 counts @08:45:13.93 SE.reconfigure SEpick @08:45:13.98 SE.seek beamBreak @08:45:16.52 SE.configure SEconfig @08:45:16.64 Elbow.reconfigure LimpElbow @08:45:16.67 Forearm.reconfigure LooseForearm @08:45:16.71 Forearm.seek 2 @08:45:16.94 Elbow.hold @08:45:16.99 Elbow.reconfigure TightElbow @08:45:17.07 SE.seek 4.03 Pucks below Top Plate @08:45:17.42 Forearm.coast @08:45:17.45 Forearm.seek 2 + 325 counts @08:45:17.70 Forearm.reconfigure TightForearm @08:45:17.73 Forearm.seek 2 + 800 counts @08:45:17.97 SE.seek 5.03 Pucks below Top Plate @08:45:18.49 Forearm.reconfigure LooseForearm @08:45:18.53 Forearm.seek retract @08:45:19.45 Elbow.seek CC @08:45:20.98 Elbow.reconfigure LooseElbow @08:45:21.07 Forearm.seek CC @08:45:22.03 CC.seek sealed @08:45:22.96 Hand.grip @08:45:23.73 CC.seek open @08:45:27.09 Forearm.seek retract @08:45:28.06 Elbow.reconfigure TightElbow @08:45:28.10 Elbow.seek 2 @08:45:29.78 Elbow.reconfigure LimpElbow @08:45:29.81 Forearm.seek 2 @08:45:30.81 Elbow.hold @08:45:30.86 Elbow.reconfigure TightElbow @08:45:30.90 Hand.release @08:45:31.68 Forearm.coast @08:45:31.71 Forearm.seek 2 + 320 counts @08:45:31.94 Forearm.reconfigure TightForearm @08:45:31.97 Forearm.seek 2 + 800 counts @08:45:32.27 SE.seek down @08:45:33.36
Forearm.reconfigure LooseForearm @08:45:33.40 Forearm.seek retract @08:45:34.48 SE.coast @08:45:34.51
SC.coast @08:45:34.53 Tranferring lysate to PRV @08:45:34.54 CSV.dial :air @08:45:34.84 CS.seek 10.000ml @08:45:34.89 CSV.dial between air and CTV @08:45:35.94 AIV.dial :bypass @08:45:36.11 Beginning samplePassC @08:45:36.12 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:45:36.26 Elbow.seek FlushPuck::Garage @08:45:38.01 Elbow.reconfigure LooseElbow @08:45:38.10 Forearm.seek garage @08:45:39.11 Hand.grip @08:45:39.94 Forearm.seek retract @08:45:40.94 Elbow.reconfigure TightElbow @08:45:40.98 Elbow.seek CC @08:45:43.79 Elbow.reconfigure LooseElbow @08:45:43.88 Forearm.reconfigure TightForearm @08:45:43.91 Forearm.seek CC @08:45:45.03 CC.seek closed @08:45:48.77 Collection Clamp onSpring/offSpring for 5/22 samples, on/off torque 2.00 @08:45:48.82 Hand.release @08:45:49.64 Forearm.reconfigure LooseForearm @08:45:49.67 Forearm.seek retract @08:45:50.63 AWV.select :air @08:45:50.64 AIV.dial :bypass @08:45:50.65 PSV.dial PSR @08:45:51.94 PSR.select :flush @08:45:52.04 PS.seek 1.500ml @08:46:08.06 delay 5 seconds @08:46:13.07 PSV.dial :waste1 @08:46:13.50 PS.seek 0.000ml @08:46:29.56 PSV.dial PSR @08:46:30.00 PSR.select :air @08:46:30.10 PS.seek 2.500ml @08:46:56.32 CBV.dial PRV @08:46:56.33 PRV.dial CBV @08:46:57.08
PSV.dial :mixloop @08:46:58.08 CTV.dial :air,avoiding => intake @08:46:58.09 PTV.dial :PRVmixing @08:46:58.17 PS.seek 0.000ml @08:47:24.34 PSV.dial :air @08:47:24.79 PS.seek 2.500ml @08:47:51.03 PSV.dial :mixloop @08:47:51.48 PS.seek 0.000ml @08:48:17.72 PSV.dial :air @08:48:18.16 PS.seek 2.500ml @08:48:44.46 PSV.dial :mixloop @08:48:44.91 PS.seek 0.000ml @08:49:11.15 Garaging FlushPuck in Collection Clamp @08:49:11.21 Elbow.reconfigure TightElbow @08:49:11.24 Elbow.seek CC @08:49:11.47 Elbow.reconfigure LooseElbow @08:49:11.56 Forearm.seek CC @08:49:12.52 CC.seek sealed @08:49:13.45 Hand.grip @08:49:14.22 CC.seek open @08:49:17.51 Forearm.seek retract @08:49:18.44 Elbow.reconfigure TightElbow @08:49:18.48 Elbow.seek garage @08:49:21.27 Elbow.reconfigure LooseElbow @08:49:21.36 Forearm.reconfigure TightForearm @08:49:21.39 Forearm.seek garage @08:49:22.57 Hand.release @08:49:23.39 Forearm.reconfigure LooseForearm @08:49:23.42 Forearm.seek retract @08:49:24.49 Connecting PS to CS to transfer sample @08:49:24.50 CBV.dial AIV @08:49:24.51 AIV.dial :bypass @08:49:24.52 PSV.dial :mixloop @08:49:24.53 PTV.dial :PRVmixing PRV.dial AIV @08:49:24.54 CTV.dial :air,avoiding => intake @08:49:24.55 Loading lyfil puck from tube 3 into Collection Clamp @08:49:24.72 SC.reconfigure SCtight @08:49:24.75 SC.seek 3 @08:49:27.01 SC.reconfigure SCloose @08:49:27.17 Elbow.reconfigure TightElbow @08:49:27.20 Elbow.seek 3 @08:49:29.75 Forearm.reconfigure TightForearm @08:49:29.78 Forearm.seek 3 + 800 counts @08:49:30.88 SE.reconfigure SEpick @08:49:30.93 SE.seek beamBreak @08:49:32.58 SE.configure SEconfig @08:49:32.70 Elbow.seek 3 @08:49:32.99 Forearm.seek 3 + 300 counts @08:49:33.26 Forearm.reconfigure LooseForearm @08:49:33.30 Hand.grip @08:49:34.10 SE.seek down @08:49:34.24
Forearm.seek retract @08:49:35.19 Elbow.seek CC @08:49:35.45 SE.coast @08:49:36.71
Elbow.reconfigure LooseElbow @08:49:36.79 Forearm.reconfigure TightForearm @08:49:36.82 Forearm.seek CC @08:49:37.95 CC.seek closed @08:49:41.70 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 2.05 @08:49:41.71 Hand.release @08:49:42.51 Forearm.reconfigure LooseForearm @08:49:42.54 Forearm.seek retract @08:49:43.46 SC.coast @08:49:43.48 CTV.dial CSV,avoiding => intake @08:49:44.77 CSV.dial CTV @08:49:45.06 CS.seek 5.000ml @08:49:58.48 delay 1:20 @08:51:18.49 CS.seek 2.500ml @08:51:25.57 delay 40 seconds @08:52:05.58 CS.seek 0.000ml @08:52:12.81 delay 1:00 @08:53:12.82 PSV.dial :air @08:53:13.26 PS.seek 2.500ml @08:53:39.45 PSV.dial :mixloop @08:53:39.89 delay 3 seconds @08:53:42.90 PSV.dial :air @08:53:43.45 delay 2 seconds @08:53:45.46 PSV.dial :mixloop @08:53:45.88 delay 3 seconds @08:53:48.89 PSV.dial :waste1 @08:53:50.18 PS.seek empty @08:54:16.45 CBV.dial :waste1 @08:54:16.90 delay 5 seconds @08:54:21.91 CBV.dial AIV @08:54:22.36 delay 10 seconds @08:54:32.37 CBV.dial :waste1 @08:54:32.84 delay 10 seconds @08:54:42.85 CBV.dial AIV @08:54:43.31 delay 10 seconds @08:54:53.32 CTV.dial CSV,avoiding => intake @08:54:53.33 CBV.dial between AIV and waste1 @08:54:53.65 CSV.dial :air @08:54:54.16 CS.seek 10.000ml @08:55:20.24 CSV.dial CTV @08:55:20.75 CS.seek 0.000ml @08:55:46.84 CBV.dial :waste1 @08:55:47.13 delay 10 seconds @08:55:57.14 CTV.dial :air,avoiding => intake @08:55:58.48 Storing lyfil puck in Collection Clamp to tube 2 @08:55:58.61 SC.reconfigure SCtight @08:55:58.64 SC.seek 2 @08:56:00.84 SC.reconfigure SCloose @08:56:01.00 Elbow.reconfigure TightElbow @08:56:01.03 Elbow.seek 2 @08:56:02.64 Forearm.reconfigure TightForearm @08:56:02.67 Forearm.seek 2 + 800 counts @08:56:03.82 SE.reconfigure SEpick @08:56:03.87 SE.seek beamBreak @08:56:06.47 SE.configure SEconfig @08:56:06.59 Elbow.reconfigure LimpElbow @08:56:06.62 Forearm.reconfigure LooseForearm @08:56:06.66 Forearm.seek 2 @08:56:06.91 Elbow.hold @08:56:06.96 Elbow.reconfigure TightElbow @08:56:07.04 SE.seek 5.03 Pucks below Top Plate @08:56:07.39 Forearm.coast @08:56:07.42 Forearm.seek 2 + 334 counts @08:56:07.64 Forearm.reconfigure TightForearm @08:56:07.68 Forearm.seek 2 + 800 counts @08:56:07.91 SE.seek 6.03 Pucks below Top Plate @08:56:08.48 Forearm.reconfigure LooseForearm @08:56:08.52 Forearm.seek retract @08:56:10.08 Elbow.seek CC @08:56:11.62 Elbow.reconfigure LooseElbow @08:56:11.71 Forearm.seek CC @08:56:12.67 CC.seek sealed @08:56:13.62 Hand.grip @08:56:14.33 CC.seek open @08:56:17.69 Forearm.seek retract @08:56:18.68 Elbow.reconfigure TightElbow @08:56:18.72 Elbow.seek 2 @08:56:20.30 Elbow.reconfigure LimpElbow @08:56:20.33 Forearm.seek 2 @08:56:21.32 Elbow.hold @08:56:21.37 Elbow.reconfigure TightElbow @08:56:21.40 Hand.release @08:56:22.16 Forearm.coast @08:56:22.19 Forearm.seek 2 + 338 counts @08:56:22.49 Forearm.reconfigure TightForearm @08:56:22.52 Forearm.seek 2 + 800 counts @08:56:22.73 SE.seek down @08:56:23.84
Forearm.reconfigure LooseForearm @08:56:23.88 Forearm.seek retract @08:56:24.85 SE.coast @08:56:24.88
SC.coast @08:56:24.90 PSV.dial :air @08:56:26.08 PS.seek 0.499ml @08:56:32.04 delay 3 seconds @08:56:35.05 PSV.dial :mixloop @08:56:35.51 PRV.dial :air @08:56:35.96 delay 3 seconds @08:56:38.97 PRV.dial CBV @08:56:39.42 CBV.dial AIV @08:56:39.88 PRV.dial between CBV and air @08:56:40.20 PRV.dial :air @08:56:40.53 PS.reconfigure PSslow @08:56:40.57 PS.seek 0.599ml @08:56:43.13 PRV.dial :waste2 @08:56:43.86 PS.reconfigure PSconfig @08:56:43.89 PS.seek empty @08:56:50.82 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:56:50.96 Elbow.seek FlushPuck::Garage @08:56:52.72 Elbow.reconfigure LooseElbow @08:56:52.81 Forearm.seek garage @08:56:53.86 Hand.grip @08:56:54.66 Forearm.seek retract @08:56:55.66 Elbow.reconfigure TightElbow @08:56:55.70 Elbow.seek CC @08:56:58.51 Elbow.reconfigure LooseElbow @08:56:58.60 Forearm.reconfigure TightForearm @08:56:58.63 Forearm.seek CC @08:56:59.74 CC.seek closed @08:57:03.55 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 2.01 @08:57:03.60 Hand.release @08:57:04.37 Forearm.reconfigure LooseForearm @08:57:04.40 Forearm.seek retract @08:57:05.37 Clearing PML for modified lysate CTV.dial :air,avoiding => intake @08:57:05.38 PRV.dial :waste2 @08:57:05.39 PTV.dial :PRV,avoiding => puck @08:57:06.10 delay 2 seconds @08:57:08.11 PTV.dial :PRVmixing,avoiding => puck @08:57:08.84 delay 2 seconds @08:57:10.85 PSV.dial :PML @08:57:10.86 delay 2 seconds @08:57:12.86 PRV.dial :air @08:57:13.59 delay 2 seconds @08:57:15.80 PS.seek 1.499ml @08:57:31.82 delay 5 seconds @08:57:36.83 PS.seek 2.500ml @08:57:47.85 PSV.dial :air @08:57:48.31 PS.seek 0.000ml @08:58:15.20 mixing lysate with speDil @08:58:15.21 PSV.dial :PML @08:58:15.65 CBV.dial :PRV @08:58:15.66 PRV.dial :air @08:58:15.67 delay 2 seconds @08:58:17.68 PRV.dial :speDil @08:58:18.11 delay 2 seconds @08:58:20.17 PS.reconfigure PSslow1 @08:58:20.20 PS.seek 0.225ml @08:58:43.54 delay 5 seconds @08:58:48.55 PRV.dial CBV @08:58:49.29 delay 2 seconds @08:58:51.35 PS.seek 0.475ml @08:59:17.35 delay 5 seconds @08:59:22.36 PRV.dial :air @08:59:22.80 delay 2 seconds @08:59:24.81 PS.seek 0.925ml @09:00:11.03 delay 10 seconds @09:00:21.04 PRV.dial CBV @09:00:21.47 CTV.dial :air,avoiding => intake @09:00:21.48 PSV.dial :air @09:00:21.93 PS.reconfigure PSconfig @09:00:21.96 PS.seek 2.500ml @09:00:38.88 delay 3 seconds @09:00:41.90 PSV.dial PTV @09:00:42.63 PTV.dial :PRV,avoiding => puck @09:00:43.37 PS.seek 0.000ml @09:01:09.70 PSV.dial :air @09:01:10.47 PS.seek 2.500ml @09:01:36.76 delay 3 seconds @09:01:39.78 PSV.dial PTV @09:01:40.49 PTV.dial :PRV,avoiding => puck @09:01:40.51 PS.seek 0.000ml @09:02:06.79 Pushing modified lysate into AIV Injection Loop @09:02:06.80 CBV.dial AIV @09:02:06.81 AIV.dial :bypass @09:02:06.82 PRV.dial CBV @09:02:06.83 PSV.dial :air @09:02:07.55 PS.seek 2.500ml @09:02:33.82 delay 3 seconds @09:02:36.83 PSV.dial :PML @09:02:37.27 PTV.dial :PRVmixing,avoiding => puck @09:02:38.07 PS.reconfigure PSslow1 @09:02:38.11 PS.seek 1.850ml @09:03:44.85 delay 5 seconds @09:03:49.86 AIV.dial :sample @09:03:50.39 AWV.select :waste @09:03:50.55 PS.reconfigure PSslow2 @09:03:50.58 PS.seek 1.310ml @09:05:40.56 delay 15 seconds @09:05:55.57 AIV.dial :bypass @09:05:56.13 PS.reconfigure PSconfig @09:05:56.16 PS.seek 0.000ml @09:06:10.31 PSV.dial :air @09:06:10.76 PS.seek 2.500ml @09:06:37.00 PSV.dial :PML @09:06:37.45 PS.seek 0.000ml @09:07:03.72 AIV.dial :coilToMFB @09:07:03.73 AWV.select :air @09:07:03.87 Beginning samplePassD @09:07:03.89 Extracting Nucleic Acid from AIV @09:07:03.91 SPE.hold 2:46:40,55.0C @09:07:03.96 ASV.dial :SPEdelivery @09:07:04.03
AWV.select :air @09:07:04.04 AIV.dial :bypass @09:07:04.05 PSV.dial PSR @09:07:04.61 delay 20 seconds @09:07:05.07
PSR.select :killMFB @09:07:05.17 PS.seek 1.500ml @09:07:21.21 delay 5 seconds @09:07:24.62 primeLysateINT @09:07:24.63 AWV.select :air @09:07:24.64 AIV.dial :coilToMFB ATV.dial :AIV @09:07:25.99 AS.reconfigure ASslow1 @09:07:26.02 AS.seek 0.239ml @09:07:26.22
PSV.dial :waste1 @09:07:26.66 PS.seek 0.000ml @09:07:42.71 PSV.dial PSR @09:07:43.17 PSR.select :air @09:07:43.27 PS.seek 2.500ml @09:08:09.56 PRV.dial CBV @09:08:09.58
CBV.dial PRV @09:08:09.59 PSV.dial :mixloop @09:08:10.74 CTV.dial :air,avoiding => intake @09:08:10.75 PTV.dial :PRVmixing @09:08:10.77 PS.seek 0.000ml @09:08:15.27 delay 10 seconds @09:08:25.28 ATV.dial :air2 @09:08:25.87 AS.seek 0.269ml @09:08:32.24 ATV.dial :waste2 @09:08:32.59 AS.reconfigure ASnorm @09:08:32.62 AS.seek empty @09:08:37.02
PSV.dial PSR @09:08:38.06 PSR.select :flush @09:08:38.16 PS.seek 1.500ml @09:08:46.80 Lysate primed! @09:08:46.81 AFV.select :holdingCoil @09:08:46.82 ATV.dial :air2 @09:08:47.20 AS.reconfigure ASslow1 @09:08:47.24 AS.seek 0.069ml @09:08:54.30
delay 5 seconds @09:08:59.32 PSV.dial :waste1 @09:08:59.77 PS.seek 0.000ml @09:09:01.72 delay 3 seconds @09:09:04.73 ATV.dial :AIV @09:09:05.30 AS.seek 0.469ml @09:09:15.87
PSV.dial PSR @09:09:16.91 PSR.select :air @09:09:17.01 PS.seek 2.500ml @09:09:43.21 PRV.dial CBV @09:09:43.23
CBV.dial PRV @09:09:43.24 PSV.dial :mixloop @09:09:44.38 CTV.dial :air,avoiding => intake @09:09:44.39 PTV.dial :PRVmixing @09:09:44.41 PS.seek 0.000ml @09:10:10.68 PSV.dial PSR @09:10:11.69 PSR.select :flush @09:10:11.79 PS.seek 1.500ml @09:10:27.17 delay 5 seconds @09:10:27.93
delay 5 seconds @09:10:32.22 Solid Phase Extraction Heater at 51.9C @09:10:32.23 ATV.dial ASV @09:10:32.67 ASV.dial :SPEdelivery @09:10:32.73 AS.seek 0.041ml @09:10:32.94
PSV.dial :waste1 @09:10:33.39 PS.seek 0.000ml @09:10:49.52 PSV.dial PSR @09:10:49.97 PSR.select :air @09:10:50.07 PS.seek 2.500ml @09:11:16.30 PRV.dial CBV @09:11:16.32
CBV.dial PRV @09:11:16.33 PSV.dial :mixloop @09:11:17.33 CTV.dial :air,avoiding => intake @09:11:17.34 PTV.dial :PRVmixing @09:11:17.36 PS.seek 0.000ml @09:11:43.61 PSV.dial PSR @09:11:44.63 PSR.select :flush @09:11:44.73 PS.seek 1.500ml @09:12:00.29 delay 10 seconds @09:12:00.89
delay 5 seconds @09:12:05.91 PSV.dial :waste1 @09:12:06.35 PS.seek 0.000ml @09:12:10.30 rinseLysateLineINT @09:12:10.31 AFV.select :carrierSolution @09:12:10.40 delay 2 seconds @09:12:12.41 AS.reconfigure ASconfig @09:12:12.44 AS.seek 1.000ml @09:12:22.42
PSV.dial PSR @09:12:22.87 PSR.select :air @09:12:22.97 PS.seek 2.500ml @09:12:49.23 PRV.dial CBV @09:12:49.25
CBV.dial PRV @09:12:49.26 PSV.dial :mixloop @09:12:50.26 CTV.dial :air,avoiding => intake @09:12:50.27 PTV.dial :PRVmixing @09:12:50.28 PS.reconfigure PSslow @09:12:50.32 PS.seek 1.200ml @09:13:08.62 delay 1 second @09:13:09.63 AFV.select :holdingCoil @09:13:09.72 delay 1 second @09:13:10.73 ATV.dial :AIV @09:13:11.06 AIV.dial :coilToMFB @09:13:11.07 AWV.select :waste @09:13:11.16 AS.reconfigure ASslow0 @09:13:11.20 AS.seek 0.000ml @09:13:17.20
PSV.dial :waste1 @09:13:18.51 PS.reconfigure PSconfig @09:13:18.54 PS.seek 0.000ml @09:13:31.76 PSV.dial :air @09:13:32.91 PS.seek 2.500ml @09:13:59.14 PSV.dial :mixloop @09:13:59.60 PS.reconfigure PSslow @09:13:59.63 PS.seek 0.000ml @09:14:50.92 PSV.dial :air @09:14:51.49 PS.reconfigure PSconfig @09:14:51.52 PS.seek 2.500ml @09:14:53.51 ATV.dial :air2 @09:14:54.18 AS.reconfigure ASslow1 @09:14:54.21 AS.seek 0.050ml @09:15:04.69 ATV.dial :AIV @09:15:05.26 AS.seek 0.010ml @09:15:13.71 ATV.dial :waste @09:15:15.00 AS.seek empty @09:15:17.44 ethanol wash @09:15:17.45 ATV.dial :air2 @09:15:17.79
PSV.dial :mixloop @09:15:18.22 PS.reconfigure PSslow @09:15:18.25 PS.seek 0.000ml @09:15:18.38 AS.reconfigure ASslow2 @09:15:18.41 AS.seek 0.009ml @09:15:28.99 delay 3 seconds @09:15:32.00 Pulling 0.4ml of Ethanol Wash @09:15:32.01 ATV.dial :ethanol @09:15:32.57 AS.reconfigure ASslow1 @09:15:32.60 AS.seek 0.409ml @09:16:09.63
Cleaning Collection Syringe @09:16:09.64 CSV.dial CSR @09:16:10.06 CSR.select :flush @09:16:10.16 CS.seek 4.000ml @09:16:21.14 delay 5 seconds @09:16:26.15 CSR.select :air @09:16:26.24 CS.seek 10.000ml @09:16:42.20 CSV.dial CTV @09:16:42.22
CTV.dial CSV @09:16:43.54 CBV.dial :waste1 @09:16:44.02 CS.seek 0.000ml @09:16:54.42 delay 3 seconds @09:16:57.43 ATV.dial :air1 @09:16:57.75 delay 3 seconds @09:17:00.81 AS.seek 0.434ml @09:17:06.14 delay 3 seconds @09:17:09.15 ATV.dial :ethanol @09:17:09.52 AS.seek 0.419ml @09:17:10.17
CSV.dial CSR @09:17:10.66 CSR.select :flush @09:17:10.76 CS.seek 4.000ml @09:17:12.81 delay 3 seconds @09:17:15.82 ATV.dial ASV @09:17:16.96 AS.seek 0.092ml @09:17:22.26
delay 5 seconds @09:17:27.27 CSR.select :air @09:17:27.36 CS.seek 10.000ml @09:17:43.32 CSV.dial CTV @09:17:43.34
CTV.dial CSV @09:17:43.85 CBV.dial :waste1 @09:17:43.87 CS.seek 0.000ml @09:18:10.22 Garaging FlushPuck in Collection Clamp @09:18:10.27 Elbow.reconfigure TightElbow @09:18:10.30 Elbow.seek CC @09:18:10.50 Elbow.reconfigure LooseElbow @09:18:10.58 Forearm.seek CC @09:18:11.54 CC.seek sealed @09:18:12.48 Hand.grip @09:18:13.17 CC.seek open @09:18:16.48 Forearm.seek retract @09:18:17.47 Elbow.reconfigure TightElbow @09:18:17.51 Elbow.seek garage @09:18:20.27 Elbow.reconfigure LooseElbow @09:18:20.35 Forearm.reconfigure TightForearm @09:18:20.38 Forearm.seek garage @09:18:21.56 Hand.release @09:18:22.33 Forearm.reconfigure LooseForearm @09:18:22.36 Forearm.seek retract @09:18:23.48 Whole Cell Archive sampling 1000ml @09:18:23.50 Loading wcr puck from tube 3 into Collection Clamp @09:18:23.67 SC.reconfigure SCtight @09:18:23.70 SC.seek 3 @09:18:23.90 delay 3 seconds @09:18:25.96 SC.reconfigure SCloose @09:18:26.14 Elbow.reconfigure TightElbow @09:18:26.17 Elbow.seek 3 @09:18:26.91 ATV.dial :waste2 @09:18:27.55 AS.reconfigure ASconfig @09:18:27.58 AS.seek empty @09:18:28.05 Forearm.reconfigure TightForearm @09:18:28.08 Forearm.seek 3 + 800 counts @09:18:29.06 SE.reconfigure SEpick @09:18:29.11 SE.seek beamBreak @09:18:30.64 SE.configure SEconfig @09:18:30.76 Elbow.seek 3 @09:18:30.92 Forearm.seek 3 + 300 counts @09:18:31.22 Forearm.reconfigure LooseForearm @09:18:31.26 Hand.grip @09:18:32.05 SE.seek down @09:18:32.19 Forearm.seek retract @09:18:33.18 Elbow.seek CC @09:18:33.29 ATV.dial :air2 @09:18:33.41 SE.coast @09:18:33.64 AS.reconfigure ASslow1 @09:18:33.67 AS.seek 1.000ml @09:18:34.70 Elbow.reconfigure LooseElbow @09:18:34.78 Forearm.reconfigure TightForearm @09:18:34.81 Forearm.seek CC @09:18:35.99 CC.seek closed @09:18:39.75 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.98 @09:18:39.76 Hand.release @09:18:40.58 Forearm.reconfigure LooseForearm @09:18:40.61 Forearm.seek retract @09:18:41.58 SC.coast @09:18:41.61 collectSample 1000ml w/bubble point of 55psi @09:18:41.63 Priming 7ml Sleepy.power :sampler,:ON @09:18:41.64 Intake.select :close Exhaust.select :close @09:18:41.65 SS.hold @09:18:42.48 Calibrating pressure sensors @09:18:42.49 CTV.dial :air @09:18:43.82 SSV.dial :spit,avoiding => exhaust @09:18:43.83 SS.reconfigure SSfast @09:18:43.86 SS.seek 5.00ml @09:18:46.62 SSV.dial CBV @09:18:46.64 CBV.dial SSV @09:18:48.07 delay 3.8 seconds @09:18:51.96 SSV.dial :spit @09:18:53.33 SS.seek 0.00ml @09:18:56.00 SSV.dial CBV @09:18:57.36 CTV.dial :intake @09:18:58.11 Waiting for external sampler to finish priming... @09:18:58.12 delayUntil "09:28:41.63" @09:21:58.10 delay 5 seconds @09:22:03.11 ATV.dial ASV @09:22:03.83 ASV.dial :SPEdelivery @09:22:03.85 AS.seek 0.020ml @09:25:24.01 delay 5 seconds @09:25:29.02 ATV.dial :air2 @09:25:29.78 AS.seek 0.520ml @09:27:12.04 delay 5 seconds @09:27:17.05 ATV.dial ASV @09:27:17.76 ASV.dial :wasteR @09:27:18.24 AS.seek 0.300ml @09:28:03.37 delay 20 seconds @09:28:23.38 AS.seek 0.070ml @09:28:41.63 SSV.dial CBV @09:28:41.65 CBV.dial SSV @09:28:41.66 Allowing 55s for 7.0ml of remaining 7.0ml @09:28:41.67 Intake.select :open @09:28:43.80 Intake @52.8psia, vacuum from 20.0 to 24.0psi @09:28:43.82 SS.reconfigure forSampling @09:28:43.85 SS.seek 7.00ml @09:28:49.54 Stroke completed -- waiting for pressure to equalize ... @09:28:50.19 Intake.select :close @09:28:50.32 SSV.dial :exhaust @09:28:51.11 Exhaust.select :open @09:28:51.22 SS.reconfigure SSexhale @09:28:51.26 SS.seek 0.00ml @09:28:54.65 Exhaust.select :close @09:28:54.77 Sampling 1000.0ml @09:28:54.78 Intake.select :close @09:28:54.79 Exhaust.select :close @09:28:54.80 SS.reconfigure SSconfig @09:28:54.83 SS.hold @09:28:54.98 CTV.dial :intake @09:28:54.99 SSV.dial CBV @09:28:55.01 CBV.dial SSV @09:28:55.82 Allowing 185s for 25.0ml of remaining 1000.0ml @09:28:55.83 Intake.select :open @09:28:57.96 Intake @52.0psia, vacuum from 20.0 to 24.0psi @09:28:57.98 SS.reconfigure forSampling @09:28:58.01 SS.seek 25.00ml @09:29:10.54 delay 20 seconds @09:29:12.85 Stroke completed -- waiting for pressure to equalize ... @09:29:13.49 Intake.select :close @09:29:13.62 SSV.dial :exhaust @09:29:14.42 Exhaust.select :open @09:29:14.53 SS.reconfigure SSexhale @09:29:14.57 SS.seek 0.00ml @09:29:23.43 Exhaust.select :close @09:29:23.54 SSV.dial CBV @09:29:23.56 CBV.dial SSV @09:29:24.35 Allowing 185s for 25.0ml of remaining 975.0ml @09:29:24.36 Intake.select :open @09:29:26.49 Intake @51.7psia, vacuum from 20.0 to 24.0psi @09:29:26.51 SS.reconfigure forSampling @09:29:26.54 SS.seek 25.00ml @09:29:30.55 ATV.dial :waste2 @09:29:31.09 AS.reconfigure ASconfig @09:29:31.12 AS.seek empty @09:29:35.55 Elution @09:29:35.56 ATV.dial :air1 @09:29:36.54 AS.reconfigure ASslow1 @09:29:36.57 AS.seek 0.049ml @09:29:41.26 Stroke completed -- waiting for pressure to equalize ... @09:29:41.90 Intake.select :close @09:29:42.03 SSV.dial :exhaust @09:29:42.82 Exhaust.select :open @09:29:42.93 SS.reconfigure SSexhale @09:29:42.97 SS.seek 0.00ml @09:29:47.07 delay 3 seconds @09:29:50.08 ATV.dial :ElutionWater @09:29:50.66 AS.seek 0.109ml @09:29:51.81 Exhaust.select :close @09:29:51.91 SSV.dial CBV @09:29:51.93 CBV.dial SSV @09:29:52.74 Allowing 185s for 25.0ml of remaining 950.0ml @09:29:52.75 Intake.select :open @09:29:54.88 Intake @52.0psia, vacuum from 20.0 to 24.0psi @09:29:54.90 SS.reconfigure forSampling @09:29:54.93 SS.seek 25.00ml @09:30:03.14 delay 3 seconds @09:30:05.15 Vacuum too high -- waiting up to 40s @09:30:05.42 Intake @43.8psia, vacuum from 20.0 to 24.0psi @09:30:05.44 SS.reconfigure forSampling @09:30:05.47 SS.seek 25.00ml @09:30:06.15 ATV.dial ASV,via => air2 @09:30:07.86 ASV.dial :SPEelution @09:30:08.54 AFV.select :carrierSolution @09:30:08.69 AS.reconfigure ASnorm @09:30:08.72 AS.seek 0.759ml @09:30:10.96 Stroke completed -- waiting for pressure to equalize ... @09:30:11.60 Intake.select :close @09:30:12.32 SSV.dial :exhaust @09:30:13.12 Exhaust.select :open @09:30:13.23 SS.reconfigure SSexhale @09:30:13.27 SS.seek 0.00ml @09:30:22.24 Exhaust.select :close @09:30:22.34 SSV.dial CBV @09:30:22.36 CBV.dial SSV @09:30:23.16 Allowing 185s for 25.0ml of remaining 925.0ml @09:30:23.17 Intake.select :open @09:30:25.31 Intake @51.8psia, vacuum from 20.0 to 24.0psi @09:30:25.33 SS.reconfigure forSampling @09:30:25.36 SS.seek 25.00ml @09:30:35.96 Vacuum too high -- waiting up to 40s @09:30:36.23 Intake @45.2psia, vacuum from 20.0 to 24.0psi @09:30:36.24 SS.reconfigure forSampling @09:30:36.28 SS.seek 25.00ml @09:30:42.22 delay 3 seconds @09:30:42.41 Stroke completed -- waiting for pressure to equalize ... @09:30:43.05 Intake.select :close @09:30:43.18 SSV.dial :exhaust @09:30:43.98 Exhaust.select :open @09:30:44.08 SS.reconfigure SSexhale @09:30:44.12 SS.seek 0.00ml @09:30:45.23 AFV.select :holdingCoil @09:30:45.37 AS.reconfigure ASslow1 @09:30:45.40 AS.seek 0.489ml @09:30:53.27 Exhaust.select :close @09:30:53.38 SSV.dial CBV @09:30:53.40 CBV.dial SSV @09:30:54.21 Allowing 185s for 25.0ml of remaining 900.0ml @09:30:54.22 Intake.select :open @09:30:56.36 Intake @50.9psia, vacuum from 20.0 to 24.0psi @09:30:56.38 SS.reconfigure forSampling @09:30:56.41 SS.seek 25.00ml @09:31:03.86 Vacuum too high -- waiting up to 40s @09:31:04.13 Intake @46.2psia, vacuum from 20.0 to 24.0psi @09:31:04.14 SS.reconfigure forSampling @09:31:04.18 SS.seek 25.00ml @09:31:15.74 Stroke completed -- waiting for pressure to equalize ... @09:31:16.38 Intake.select :close @09:31:16.51 SSV.dial :exhaust @09:31:17.32 Exhaust.select :open @09:31:17.43 SS.reconfigure SSexhale @09:31:17.47 SS.seek 0.00ml @09:31:26.54 Exhaust.select :close @09:31:26.65 SSV.dial CBV @09:31:26.67 CBV.dial SSV @09:31:27.47 Allowing 185s for 25.0ml of remaining 875.0ml @09:31:27.48 Intake.select :open @09:31:29.62 Intake @51.5psia, vacuum from 20.0 to 24.0psi @09:31:29.64 SS.reconfigure forSampling @09:31:29.67 SS.seek 25.00ml @09:31:33.30 Vacuum too high -- waiting up to 40s @09:31:33.56 Intake @46.9psia, vacuum from 20.0 to 24.0psi @09:31:33.58 SS.reconfigure forSampling @09:31:33.61 SS.seek 25.00ml @09:31:40.67 delay 5 seconds @09:31:45.21 Vacuum too high -- waiting up to 40s @09:31:45.68 Intake @49.8psia, vacuum from 20.0 to 24.0psi @09:31:45.69 SS.reconfigure forSampling @09:31:45.72 SS.seek 25.00ml @09:31:45.80 AS.reconfigure ASslow2 @09:31:45.84 AS.seek 0.454ml @09:31:50.42 Vacuum too high -- waiting up to 40s @09:31:50.68 Intake @48.8psia, vacuum from 20.0 to 24.0psi @09:31:50.70 SS.reconfigure forSampling @09:31:50.73 SS.seek 25.00ml @09:31:56.13 Stroke completed -- waiting for pressure to equalize ... @09:31:56.76 Intake.select :close @09:31:56.91 SSV.dial :exhaust @09:31:57.71 Exhaust.select :open @09:31:57.84 SS.reconfigure SSexhale @09:31:57.92 SS.seek 0.00ml @09:32:07.00 Exhaust.select :close @09:32:07.11 SSV.dial CBV @09:32:07.13 CBV.dial SSV @09:32:07.94 Allowing 185s for 25.0ml of remaining 850.0ml @09:32:07.95 Intake.select :open @09:32:10.09 Intake @51.0psia, vacuum from 20.0 to 24.0psi @09:32:10.11 SS.reconfigure forSampling @09:32:10.14 SS.seek 25.00ml @09:32:19.89 Vacuum too high -- waiting up to 40s @09:32:20.15 Intake @49.4psia, vacuum from 20.0 to 24.0psi @09:32:20.17 SS.reconfigure forSampling @09:32:20.20 SS.seek 25.00ml @09:32:21.59 delay 1:00 @09:32:48.20 Stroke completed -- waiting for pressure to equalize ... @09:32:49.41 Intake.select :close @09:32:49.55 SSV.dial :exhaust @09:32:50.48 Exhaust.select :open @09:32:50.57 SS.reconfigure SSexhale @09:32:50.61 SS.seek 0.00ml @09:32:59.75 Exhaust.select :close @09:32:59.86 SSV.dial CBV @09:32:59.88 CBV.dial SSV @09:33:00.69 Allowing 185s for 25.0ml of remaining 825.0ml @09:33:00.70 Intake.select :open @09:33:03.45 Intake @51.6psia, vacuum from 20.0 to 24.0psi @09:33:03.47 SS.reconfigure forSampling @09:33:03.50 SS.seek 25.00ml @09:33:06.49 Vacuum too high -- waiting up to 40s @09:33:06.76 Intake @50.1psia, vacuum from 20.0 to 24.0psi @09:33:06.77 SS.reconfigure forSampling @09:33:06.81 SS.seek 25.00ml @09:33:21.60 pushing eluate to holding coil @09:33:21.66 AS.seek 0.294ml @09:34:02.43 Stroke completed -- waiting for pressure to equalize ... @09:34:03.66 Intake.select :close @09:34:03.79 SSV.dial :exhaust @09:34:04.61 Exhaust.select :open @09:34:04.72 SS.reconfigure SSexhale @09:34:04.76 SS.seek 0.00ml @09:34:13.90 Exhaust.select :close @09:34:14.00 SSV.dial CBV @09:34:14.02 CBV.dial SSV @09:34:14.84 Allowing 185s for 25.0ml of remaining 800.0ml @09:34:14.85 Intake.select :open @09:34:16.99 Intake @50.2psia, vacuum from 20.0 to 24.0psi @09:34:17.01 SS.reconfigure forSampling @09:34:17.04 SS.seek 25.00ml @09:34:25.85 Vacuum too high -- waiting up to 40s @09:34:26.33 Intake @50.0psia, vacuum from 20.0 to 24.0psi @09:34:26.34 SS.reconfigure forSampling @09:34:26.38 SS.seek 25.00ml @09:36:01.09 Stroke completed -- waiting for pressure to equalize ... @09:36:02.90 Intake.select :close @09:36:03.03 SSV.dial :exhaust @09:36:03.83 Exhaust.select :open @09:36:03.94 SS.reconfigure SSexhale @09:36:03.98 SS.seek 0.00ml @09:36:05.03 delay 30 seconds @09:36:13.12 Exhaust.select :close @09:36:13.23 SSV.dial CBV @09:36:13.25 CBV.dial SSV @09:36:14.05 Allowing 185s for 25.0ml of remaining 775.0ml @09:36:14.06 Intake.select :open @09:36:16.20 Intake @50.3psia, vacuum from 20.0 to 24.0psi @09:36:16.22 SS.reconfigure forSampling @09:36:16.25 SS.seek 25.00ml @09:36:35.03 ATV.dial :waste,via => air3 @09:36:36.56 AS.reconfigure ASconfig @09:36:36.59 AS.seek empty @09:36:54.08 Parking Bleach in LysateSource line and SPEcolumn @09:36:54.09 ATV.dial :air3 @09:36:54.77 AS.reconfigure ASslow1 @09:36:54.80 AS.seek 0.029ml @09:37:01.17 ATV.dial :ElutionWater @09:37:01.57 AS.seek 0.059ml @09:37:07.92 ATV.dial :air3 @09:37:08.32 AS.seek 0.069ml @09:37:10.63 delay 2 seconds @09:37:12.64 ATV.dial :bleach @09:37:13.13 AS.seek 0.709ml @09:38:06.60 Stroke completed -- waiting for pressure to equalize ... @09:38:08.97 Intake.select :close @09:38:09.12 SSV.dial :exhaust @09:38:09.92 Exhaust.select :open @09:38:10.02 SS.reconfigure SSexhale @09:38:10.06 SS.seek 0.00ml @09:38:19.09 Exhaust.select :close @09:38:19.20 SSV.dial CBV @09:38:19.22 CBV.dial SSV @09:38:20.05 Allowing 185s for 25.0ml of remaining 750.0ml @09:38:20.06 Intake.select :open @09:38:22.19 Intake @49.8psia, vacuum from 20.0 to 24.0psi @09:38:22.20 SS.reconfigure forSampling @09:38:22.24 SS.seek 25.00ml @09:38:53.03 Vacuum too high -- waiting up to 40s @09:38:53.50 Intake @49.6psia, vacuum from 20.0 to 24.0psi @09:38:53.52 SS.reconfigure forSampling @09:38:53.55 SS.seek 25.00ml @09:39:04.66 Vacuum too high -- waiting up to 40s @09:39:05.14 Intake @49.9psia, vacuum from 20.0 to 24.0psi @09:39:05.15 SS.reconfigure forSampling @09:39:05.18 SS.seek 25.00ml @09:39:08.60 Vacuum too high -- waiting up to 40s @09:39:09.07 Intake @49.7psia, vacuum from 20.0 to 24.0psi @09:39:09.09 SS.reconfigure forSampling @09:39:09.12 SS.seek 25.00ml @09:39:12.57 Vacuum too high -- waiting up to 40s @09:39:13.05 Intake @49.7psia, vacuum from 20.0 to 24.0psi @09:39:13.06 SS.reconfigure forSampling @09:39:13.09 SS.seek 25.00ml @09:39:16.81 Vacuum too high -- waiting up to 40s @09:39:17.72 Intake @50.9psia, vacuum from 20.0 to 24.0psi @09:39:17.73 SS.reconfigure forSampling @09:39:17.76 SS.seek 25.00ml @09:39:21.50 Vacuum too high -- waiting up to 40s @09:39:21.99 Intake @50.1psia, vacuum from 20.0 to 24.0psi @09:39:22.00 SS.reconfigure forSampling @09:39:22.04 SS.seek 25.00ml @09:39:23.90 delay 3 seconds @09:39:25.23 Vacuum too high -- waiting up to 40s @09:39:25.71 Intake @49.9psia, vacuum from 20.0 to 24.0psi @09:39:25.72 SS.reconfigure forSampling @09:39:25.76 SS.seek 25.00ml @09:39:26.91 ATV.dial :air3 @09:39:27.35 delay 3 seconds @09:39:28.67 Vacuum too high -- waiting up to 40s @09:39:29.16 Intake @49.6psia, vacuum from 20.0 to 24.0psi @09:39:29.18 SS.reconfigure forSampling @09:39:29.21 SS.seek 25.00ml @09:39:30.40 AS.seek 0.734ml @09:39:31.98 Vacuum too high -- waiting up to 40s @09:39:32.47 Intake @49.8psia, vacuum from 20.0 to 24.0psi @09:39:32.49 SS.reconfigure forSampling @09:39:32.52 SS.seek 25.00ml @09:39:35.48 Vacuum too high -- waiting up to 40s @09:39:35.75 delay 3 seconds @09:39:35.97 Intake @51.0psia, vacuum from 20.0 to 24.0psi @09:39:35.98 SS.reconfigure forSampling @09:39:36.02 SS.seek 25.00ml @09:39:38.26 Vacuum too high -- waiting up to 40s @09:39:38.73 Intake @49.8psia, vacuum from 20.0 to 24.0psi @09:39:38.74 SS.reconfigure forSampling @09:39:38.77 ATV.dial :bleach @09:39:38.79 SS.seek 25.00ml @09:39:39.20 AS.seek 0.719ml @09:39:41.51 Vacuum too high -- waiting up to 40s @09:39:42.00 Intake @50.2psia, vacuum from 20.0 to 24.0psi @09:39:42.01 SS.reconfigure forSampling @09:39:42.05 SS.seek 25.00ml @09:39:42.50 delay 3 seconds @09:39:44.60 Vacuum too high -- waiting up to 40s @09:39:45.08 Intake @50.4psia, vacuum from 20.0 to 24.0psi @09:39:45.10 SS.reconfigure forSampling @09:39:45.13 SS.seek 25.00ml @09:39:45.51 ATV.dial :AIV @09:39:46.66 AS.reconfigure ASconfig @09:39:46.69 AS.seek 0.015ml @09:39:47.29 Vacuum too high -- waiting up to 40s @09:39:47.76 Intake @49.9psia, vacuum from 20.0 to 24.0psi @09:39:47.77 SS.reconfigure forSampling @09:39:47.81 SS.seek 25.00ml @09:39:50.10 Vacuum too high -- waiting up to 40s @09:39:50.58 Intake @49.9psia, vacuum from 20.0 to 24.0psi @09:39:50.59 SS.reconfigure forSampling @09:39:50.63 SS.seek 25.00ml @09:39:53.07 Vacuum too high -- waiting up to 40s @09:39:53.54 Intake @49.9psia, vacuum from 20.0 to 24.0psi @09:39:53.55 SS.reconfigure forSampling @09:39:53.60 SS.seek 25.00ml @09:39:56.04 Vacuum too high -- waiting up to 40s @09:39:56.52 Intake @50.0psia, vacuum from 20.0 to 24.0psi @09:39:56.53 SS.reconfigure forSampling @09:39:56.57 SS.seek 25.00ml @09:39:58.60 Vacuum too high -- waiting up to 40s @09:39:59.06 Intake @49.5psia, vacuum from 20.0 to 24.0psi @09:39:59.08 SS.reconfigure forSampling @09:39:59.11 SS.seek 25.00ml @09:40:01.48 Vacuum too high -- waiting up to 40s @09:40:01.96 Intake @49.6psia, vacuum from 20.0 to 24.0psi @09:40:01.97 SS.reconfigure forSampling @09:40:02.01 SS.seek 25.00ml @09:40:04.01 Vacuum too high -- waiting up to 40s @09:40:04.48 Intake @50.2psia, vacuum from 20.0 to 24.0psi @09:40:04.49 SS.reconfigure forSampling @09:40:04.52 SS.seek 25.00ml @09:40:06.60 Vacuum too high -- waiting up to 40s @09:40:07.54 Intake @50.3psia, vacuum from 20.0 to 24.0psi @09:40:07.55 SS.reconfigure forSampling @09:40:07.58 SS.seek 25.00ml @09:40:09.82 Vacuum too high -- waiting up to 40s @09:40:10.32 Intake @49.8psia, vacuum from 20.0 to 24.0psi @09:40:10.33 SS.reconfigure forSampling @09:40:10.36 SS.seek 25.00ml @09:40:12.57 Vacuum too high -- waiting up to 40s @09:40:13.05 Intake @49.7psia, vacuum from 20.0 to 24.0psi @09:40:13.07 SS.reconfigure forSampling @09:40:13.10 SS.seek 25.00ml @09:40:15.01 Vacuum too high -- waiting up to 40s @09:40:15.50 Intake @50.2psia, vacuum from 20.0 to 24.0psi @09:40:15.51 SS.reconfigure forSampling @09:40:15.55 SS.seek 25.00ml @09:40:17.63 Vacuum too high -- waiting up to 40s @09:40:18.11 Intake @50.1psia, vacuum from 20.0 to 24.0psi @09:40:18.13 SS.reconfigure forSampling @09:40:18.16 SS.seek 25.00ml @09:40:19.98 Vacuum too high -- waiting up to 40s @09:40:20.47 Intake @49.6psia, vacuum from 20.0 to 24.0psi @09:40:20.49 SS.reconfigure forSampling @09:40:20.52 SS.seek 25.00ml @09:40:22.60 Vacuum too high -- waiting up to 40s @09:40:23.09 Intake @49.6psia, vacuum from 20.0 to 24.0psi @09:40:23.10 SS.reconfigure forSampling @09:40:23.14 SS.seek 25.00ml @09:40:24.91 Vacuum too high -- waiting up to 40s @09:40:25.40 Intake @49.5psia, vacuum from 20.0 to 24.0psi @09:40:25.41 SS.reconfigure forSampling @09:40:25.45 SS.seek 25.00ml @09:40:27.54 Vacuum too high -- waiting up to 40s @09:40:28.23 Intake @50.1psia, vacuum from 20.0 to 24.0psi @09:40:28.24 SS.reconfigure forSampling @09:40:28.28 SS.seek 25.00ml @09:40:28.84 ATV.dial :waste @09:40:30.18 AS.seek empty @09:40:30.51 Vacuum too high -- waiting up to 40s @09:40:30.99 Intake @50.0psia, vacuum from 20.0 to 24.0psi @09:40:31.00 SS.reconfigure forSampling @09:40:31.04 SS.seek 25.00ml @09:40:31.37 ATV.dial :air3 @09:40:31.97 AS.reconfigure ASslow1 @09:40:32.00 AS.seek 0.009ml @09:40:32.91 Vacuum too high -- waiting up to 40s @09:40:33.40 Intake @49.8psia, vacuum from 20.0 to 24.0psi @09:40:33.41 SS.reconfigure forSampling @09:40:33.45 SS.seek 25.00ml @09:40:34.19 Stroke completed -- waiting for pressure to equalize ... @09:40:34.37 delay 2 seconds @09:40:36.38 ATV.dial :bleach @09:40:36.57 Intake.select :close @09:40:36.72 SSV.dial :exhaust @09:40:36.81 AS.seek 0.579ml @09:40:37.52 Exhaust.select :open @09:40:37.62 SS.reconfigure SSexhale @09:40:37.66 SS.seek 0.00ml @09:40:46.74 Exhaust.select :close @09:40:46.85 SSV.dial CBV @09:40:46.87 CBV.dial SSV @09:40:47.67 Allowing 185s for 25.0ml of remaining 725.0ml @09:40:47.69 Intake.select :open @09:40:49.83 Intake @50.5psia, vacuum from 20.0 to 24.0psi @09:40:49.85 SS.reconfigure forSampling @09:40:49.88 SS.seek 25.00ml @09:40:53.06 Vacuum too high -- waiting up to 40s @09:40:53.54 Intake @51.0psia, vacuum from 20.0 to 24.0psi @09:40:53.56 SS.reconfigure forSampling @09:40:53.59 SS.seek 25.00ml @09:40:55.32 Vacuum too high -- waiting up to 40s @09:40:55.80 Intake @50.5psia, vacuum from 20.0 to 24.0psi @09:40:55.82 SS.reconfigure forSampling @09:40:55.85 SS.seek 25.00ml @09:40:57.79 Vacuum too high -- waiting up to 40s @09:40:58.95 Intake @50.3psia, vacuum from 20.0 to 24.0psi @09:40:58.96 SS.reconfigure forSampling @09:40:58.99 SS.seek 25.00ml @09:41:01.44 Vacuum too high -- waiting up to 40s @09:41:01.96 Intake @50.0psia, vacuum from 20.0 to 24.0psi @09:41:01.97 SS.reconfigure forSampling @09:41:02.00 SS.seek 25.00ml @09:41:03.85 Vacuum too high -- waiting up to 40s @09:41:04.34 Intake @49.6psia, vacuum from 20.0 to 24.0psi @09:41:04.36 SS.reconfigure forSampling @09:41:04.39 SS.seek 25.00ml @09:41:06.26 Vacuum too high -- waiting up to 40s @09:41:06.75 Intake @49.9psia, vacuum from 20.0 to 24.0psi @09:41:06.77 SS.reconfigure forSampling @09:41:06.80 SS.seek 25.00ml @09:41:09.01 Vacuum too high -- waiting up to 40s @09:41:09.71 Intake @50.8psia, vacuum from 20.0 to 24.0psi @09:41:09.72 SS.reconfigure forSampling @09:41:09.76 SS.seek 25.00ml @09:41:11.76 Vacuum too high -- waiting up to 40s @09:41:12.25 Intake @50.0psia, vacuum from 20.0 to 24.0psi @09:41:12.26 SS.reconfigure forSampling @09:41:12.29 SS.seek 25.00ml @09:41:14.13 Vacuum too high -- waiting up to 40s @09:41:14.60 Intake @49.6psia, vacuum from 20.0 to 24.0psi @09:41:14.61 SS.reconfigure forSampling @09:41:14.64 SS.seek 25.00ml @09:41:16.41 Vacuum too high -- waiting up to 40s @09:41:16.88 Intake @50.0psia, vacuum from 20.0 to 24.0psi @09:41:16.89 SS.reconfigure forSampling @09:41:16.92 SS.seek 25.00ml @09:41:18.69 Vacuum too high -- waiting up to 40s @09:41:19.17 Intake @49.7psia, vacuum from 20.0 to 24.0psi @09:41:19.18 SS.reconfigure forSampling @09:41:19.22 SS.seek 25.00ml @09:41:20.88 Vacuum too high -- waiting up to 40s @09:41:21.36 Intake @49.6psia, vacuum from 20.0 to 24.0psi @09:41:21.37 SS.reconfigure forSampling @09:41:21.40 SS.seek 25.00ml @09:41:23.00 Vacuum too high -- waiting up to 40s @09:41:23.48 Intake @49.5psia, vacuum from 20.0 to 24.0psi @09:41:23.49 SS.reconfigure forSampling @09:41:23.53 SS.seek 25.00ml @09:41:25.29 Vacuum too high -- waiting up to 40s @09:41:25.75 Intake @49.9psia, vacuum from 20.0 to 24.0psi @09:41:25.77 SS.reconfigure forSampling @09:41:25.80 SS.seek 25.00ml @09:41:27.41 Vacuum too high -- waiting up to 40s @09:41:27.90 Intake @50.4psia, vacuum from 20.0 to 24.0psi @09:41:27.91 SS.reconfigure forSampling @09:41:27.95 SS.seek 25.00ml @09:41:29.54 Vacuum too high -- waiting up to 40s @09:41:30.01 Intake @49.8psia, vacuum from 20.0 to 24.0psi @09:41:30.02 SS.reconfigure forSampling @09:41:30.06 SS.seek 25.00ml @09:41:31.72 Vacuum too high -- waiting up to 40s @09:41:32.19 Intake @49.9psia, vacuum from 20.0 to 24.0psi @09:41:32.20 SS.reconfigure forSampling @09:41:32.24 SS.seek 25.00ml @09:41:33.88 Vacuum too high -- waiting up to 40s @09:41:34.36 Intake @49.8psia, vacuum from 20.0 to 24.0psi @09:41:34.37 SS.reconfigure forSampling @09:41:34.40 SS.seek 25.00ml @09:41:36.00 Vacuum too high -- waiting up to 40s @09:41:36.48 Intake @49.7psia, vacuum from 20.0 to 24.0psi @09:41:36.49 SS.reconfigure forSampling @09:41:36.53 SS.seek 25.00ml @09:41:37.97 Vacuum too high -- waiting up to 40s @09:41:38.44 Intake @49.6psia, vacuum from 20.0 to 24.0psi @09:41:38.45 SS.reconfigure forSampling @09:41:38.48 SS.seek 25.00ml @09:41:41.47 Vacuum too high -- waiting up to 40s @09:41:41.96 Intake @49.7psia, vacuum from 20.0 to 24.0psi @09:41:41.97 SS.reconfigure forSampling @09:41:42.01 SS.seek 25.00ml @09:41:43.47 Vacuum too high -- waiting up to 40s @09:41:44.18 Intake @49.9psia, vacuum from 20.0 to 24.0psi @09:41:44.20 SS.reconfigure forSampling @09:41:44.23 SS.seek 25.00ml @09:41:46.44 Vacuum too high -- waiting up to 40s @09:41:47.15 Intake @50.7psia, vacuum from 20.0 to 24.0psi @09:41:47.17 SS.reconfigure forSampling @09:41:47.20 SS.seek 25.00ml @09:41:48.69 Vacuum too high -- waiting up to 40s @09:41:49.17 Intake @50.0psia, vacuum from 20.0 to 24.0psi @09:41:49.19 SS.reconfigure forSampling @09:41:49.22 SS.seek 25.00ml @09:41:50.78 Vacuum too high -- waiting up to 40s @09:41:51.27 Intake @49.7psia, vacuum from 20.0 to 24.0psi @09:41:51.28 SS.reconfigure forSampling @09:41:51.31 SS.seek 25.00ml @09:41:52.75 Vacuum too high -- waiting up to 40s @09:41:53.23 Intake @49.6psia, vacuum from 20.0 to 24.0psi @09:41:53.24 SS.reconfigure forSampling @09:41:53.28 SS.seek 25.00ml @09:41:54.75 Vacuum too high -- waiting up to 40s @09:41:55.45 Intake @49.9psia, vacuum from 20.0 to 24.0psi @09:41:55.46 SS.reconfigure forSampling @09:41:55.49 SS.seek 25.00ml @09:41:57.25 Vacuum too high -- waiting up to 40s @09:41:57.72 Intake @49.8psia, vacuum from 20.0 to 24.0psi @09:41:57.73 SS.reconfigure forSampling @09:41:57.77 SS.seek 25.00ml @09:41:59.10 Vacuum too high -- waiting up to 40s @09:41:59.57 Intake @49.7psia, vacuum from 20.0 to 24.0psi @09:41:59.58 SS.reconfigure forSampling @09:41:59.62 SS.seek 25.00ml @09:42:01.07 Vacuum too high -- waiting up to 40s @09:42:01.76 Intake @50.4psia, vacuum from 20.0 to 24.0psi @09:42:01.77 SS.reconfigure forSampling @09:42:01.81 SS.seek 25.00ml @09:42:03.38 Vacuum too high -- waiting up to 40s @09:42:03.85 Intake @51.1psia, vacuum from 20.0 to 24.0psi @09:42:03.87 SS.reconfigure forSampling @09:42:03.90 SS.seek 25.00ml @09:42:05.13 Vacuum too high -- waiting up to 40s @09:42:05.60 Intake @50.1psia, vacuum from 20.0 to 24.0psi @09:42:05.61 SS.reconfigure forSampling @09:42:05.64 SS.seek 25.00ml @09:42:07.03 Vacuum too high -- waiting up to 40s @09:42:07.51 Intake @49.9psia, vacuum from 20.0 to 24.0psi @09:42:07.52 SS.reconfigure forSampling @09:42:07.55 SS.seek 25.00ml @09:42:08.97 Vacuum too high -- waiting up to 40s @09:42:09.45 Intake @49.8psia, vacuum from 20.0 to 24.0psi @09:42:09.46 SS.reconfigure forSampling @09:42:09.50 SS.seek 25.00ml @09:42:10.95 Vacuum too high -- waiting up to 40s @09:42:11.43 Intake @50.1psia, vacuum from 20.0 to 24.0psi @09:42:11.44 SS.reconfigure forSampling @09:42:11.48 SS.seek 25.00ml @09:42:12.91 Vacuum too high -- waiting up to 40s @09:42:13.38 Intake @50.0psia, vacuum from 20.0 to 24.0psi @09:42:13.39 SS.reconfigure forSampling @09:42:13.43 SS.seek 25.00ml @09:42:14.81 Vacuum too high -- waiting up to 40s @09:42:15.30 Intake @49.8psia, vacuum from 20.0 to 24.0psi @09:42:15.31 SS.reconfigure forSampling @09:42:16.03 SS.seek 25.00ml @09:42:18.06 Vacuum too high -- waiting up to 40s @09:42:18.55 Intake @49.7psia, vacuum from 20.0 to 24.0psi @09:42:18.57 SS.reconfigure forSampling @09:42:18.60 SS.seek 25.00ml @09:42:20.00 Vacuum too high -- waiting up to 40s @09:42:20.71 Intake @50.9psia, vacuum from 20.0 to 24.0psi @09:42:20.73 SS.reconfigure forSampling @09:42:20.76 SS.seek 25.00ml @09:42:22.16 Vacuum too high -- waiting up to 40s @09:42:22.64 Intake @50.3psia, vacuum from 20.0 to 24.0psi @09:42:22.66 SS.reconfigure forSampling @09:42:22.69 SS.seek 25.00ml @09:42:24.03 Vacuum too high -- waiting up to 40s @09:42:24.51 Intake @49.8psia, vacuum from 20.0 to 24.0psi @09:42:24.53 SS.reconfigure forSampling @09:42:24.56 SS.seek 25.00ml @09:42:25.97 Vacuum too high -- waiting up to 40s @09:42:26.45 Intake @49.6psia, vacuum from 20.0 to 24.0psi @09:42:26.46 SS.reconfigure forSampling @09:42:26.50 SS.seek 25.00ml @09:42:27.78 Vacuum too high -- waiting up to 40s @09:42:28.47 Intake @49.5psia, vacuum from 20.0 to 24.0psi @09:42:28.48 SS.reconfigure forSampling @09:42:28.52 SS.seek 25.00ml @09:42:30.13 Vacuum too high -- waiting up to 40s @09:42:30.82 Intake @49.8psia, vacuum from 20.0 to 24.0psi @09:42:30.83 SS.reconfigure forSampling @09:42:30.87 SS.seek 25.00ml @09:42:32.44 Vacuum too high -- waiting up to 40s @09:42:33.13 Intake @50.0psia, vacuum from 20.0 to 24.0psi @09:42:33.14 SS.reconfigure forSampling @09:42:33.18 SS.seek 25.00ml @09:42:33.27 delay 3 seconds @09:42:34.63 Vacuum too high -- waiting up to 40s @09:42:35.32 Intake @49.9psia, vacuum from 20.0 to 24.0psi @09:42:35.33 SS.reconfigure forSampling @09:42:35.37 SS.seek 25.00ml @09:42:36.28 ATV.dial :air3 @09:42:36.63 delay 3 seconds @09:42:36.91 Vacuum too high -- waiting up to 40s @09:42:37.40 SS.seek 25.00ml @09:42:38.63 Vacuum too high -- waiting up to 40s @09:42:39.30 Intake @50.6psia, vacuum from 20.0 to 24.0psi @09:42:39.32 SS.reconfigure forSampling @09:42:39.35 SS.seek 25.00ml @09:42:39.69 AS.seek 0.604ml @09:42:40.63 Vacuum too high -- waiting up to 40s @09:42:41.12 Intake @50.6psia, vacuum from 20.0 to 24.0psi @09:42:41.13 SS.reconfigure forSampling @09:42:41.17 SS.seek 25.00ml @09:42:42.47 Vacuum too high -- waiting up to 40s @09:42:42.95 Intake @50.2psia, vacuum from 20.0 to 24.0psi @09:42:42.96 SS.reconfigure forSampling @09:42:42.99 SS.seek 25.00ml @09:42:44.22 Vacuum too high -- waiting up to 40s @09:42:44.69 Intake @49.9psia, vacuum from 20.0 to 24.0psi @09:42:44.70 SS.reconfigure forSampling @09:42:44.74 SS.seek 25.00ml @09:42:45.05 delay 3 seconds @09:42:45.94 Vacuum too high -- waiting up to 40s @09:42:46.63 Intake @49.8psia, vacuum from 20.0 to 24.0psi @09:42:46.65 SS.reconfigure forSampling @09:42:46.68 SS.seek 25.00ml @09:42:48.06 ATV.dial :bleach @09:42:48.28 Vacuum too high -- waiting up to 40s @09:42:48.45 AS.seek 0.589ml @09:42:49.31 Intake @49.7psia, vacuum from 20.0 to 24.0psi @09:42:49.32 SS.reconfigure forSampling @09:42:49.36 SS.seek 25.00ml @09:42:51.03 Vacuum too high -- waiting up to 40s @09:42:51.74 Intake @49.6psia, vacuum from 20.0 to 24.0psi @09:42:51.76 SS.reconfigure forSampling @09:42:51.79 SS.seek 25.00ml @09:42:51.84 delay 3 seconds @09:42:53.22 Vacuum too high -- waiting up to 40s @09:42:53.92 Intake @49.5psia, vacuum from 20.0 to 24.0psi @09:42:53.94 SS.reconfigure forSampling @09:42:53.97 SS.seek 25.00ml @09:42:54.84 ATV.dial :ASV @09:42:55.69 Vacuum too high -- waiting up to 40s @09:42:55.77 ASV.dial :wasteR @09:42:56.39 Intake @50.8psia, vacuum from 20.0 to 24.0psi @09:42:56.40 SS.reconfigure forSampling @09:42:56.44 SS.seek 25.00ml @09:42:56.73 AS.seek 0.000ml @09:42:57.59 Vacuum too high -- waiting up to 40s @09:42:58.08 Intake @50.2psia, vacuum from 20.0 to 24.0psi @09:42:58.09 SS.reconfigure forSampling @09:42:58.13 SS.seek 25.00ml @09:42:59.28 Vacuum too high -- waiting up to 40s @09:42:59.75 Intake @49.6psia, vacuum from 20.0 to 24.0psi @09:42:59.77 SS.reconfigure forSampling @09:42:59.80 SS.seek 25.00ml @09:43:01.12 Vacuum too high -- waiting up to 40s @09:43:01.82 Intake @49.5psia, vacuum from 20.0 to 24.0psi @09:43:01.83 SS.reconfigure forSampling @09:43:01.87 SS.seek 25.00ml @09:43:03.28 Vacuum too high -- waiting up to 40s @09:43:03.96 Intake @49.2psia, vacuum from 20.0 to 24.0psi @09:43:03.97 SS.reconfigure forSampling @09:43:04.00 SS.seek 25.00ml @09:43:05.53 Vacuum too high -- waiting up to 40s @09:43:06.21 Intake @49.7psia, vacuum from 20.0 to 24.0psi @09:43:06.23 SS.reconfigure forSampling @09:43:06.26 SS.seek 25.00ml @09:43:07.66 Vacuum too high -- waiting up to 40s @09:43:08.35 Intake @50.4psia, vacuum from 20.0 to 24.0psi @09:43:08.36 SS.reconfigure forSampling @09:43:08.40 SS.seek 25.00ml @09:43:09.56 Vacuum too high -- waiting up to 40s @09:43:10.04 Intake @49.7psia, vacuum from 20.0 to 24.0psi @09:43:10.05 SS.reconfigure forSampling @09:43:10.08 SS.seek 25.00ml @09:43:11.28 Vacuum too high -- waiting up to 40s @09:43:11.97 Intake @49.8psia, vacuum from 20.0 to 24.0psi @09:43:11.98 SS.reconfigure forSampling @09:43:12.02 SS.seek 25.00ml @09:43:13.44 Vacuum too high -- waiting up to 40s @09:43:14.12 Intake @50.5psia, vacuum from 20.0 to 24.0psi @09:43:14.14 SS.reconfigure forSampling @09:43:14.17 SS.seek 25.00ml @09:43:15.37 Vacuum too high -- waiting up to 40s @09:43:16.07 Intake @50.5psia, vacuum from 20.0 to 24.0psi @09:43:16.08 SS.reconfigure forSampling @09:43:16.12 SS.seek 25.00ml @09:43:17.47 Vacuum too high -- waiting up to 40s @09:43:17.95 Intake @49.9psia, vacuum from 20.0 to 24.0psi @09:43:17.96 SS.reconfigure forSampling @09:43:17.99 SS.seek 25.00ml @09:43:19.34 Vacuum too high -- waiting up to 40s @09:43:20.49 Intake @49.6psia, vacuum from 20.0 to 24.0psi @09:43:20.51 SS.reconfigure forSampling @09:43:20.54 SS.seek 25.00ml @09:43:22.34 Vacuum too high -- waiting up to 40s @09:43:23.05 Intake @49.1psia, vacuum from 20.0 to 24.0psi @09:43:23.07 SS.reconfigure forSampling @09:43:23.10 SS.seek 25.00ml @09:43:24.53 Vacuum too high -- waiting up to 40s @09:43:25.24 Intake @49.5psia, vacuum from 20.0 to 24.0psi @09:43:25.26 SS.reconfigure forSampling @09:43:25.29 SS.seek 25.00ml @09:43:26.69 Vacuum too high -- waiting up to 40s @09:43:27.39 Intake @50.5psia, vacuum from 20.0 to 24.0psi @09:43:27.40 SS.reconfigure forSampling @09:43:27.44 SS.seek 25.00ml @09:43:28.59 Vacuum too high -- waiting up to 40s @09:43:29.29 Intake @50.6psia, vacuum from 20.0 to 24.0psi @09:43:29.30 SS.reconfigure forSampling @09:43:29.34 SS.seek 25.00ml @09:43:30.79 Vacuum too high -- waiting up to 40s @09:43:31.29 Intake @50.9psia, vacuum from 20.0 to 24.0psi @09:43:31.30 SS.reconfigure forSampling @09:43:31.34 SS.seek 25.00ml @09:43:32.65 Vacuum too high -- waiting up to 40s @09:43:33.13 Intake @50.6psia, vacuum from 20.0 to 24.0psi @09:43:33.15 SS.reconfigure forSampling @09:43:33.18 SS.seek 25.00ml @09:43:34.19 Vacuum too high -- waiting up to 40s @09:43:34.88 Intake @50.2psia, vacuum from 20.0 to 24.0psi @09:43:34.89 SS.reconfigure forSampling @09:43:34.93 SS.seek 25.00ml @09:43:36.31 Vacuum too high -- waiting up to 40s @09:43:37.00 Intake @49.9psia, vacuum from 20.0 to 24.0psi @09:43:37.01 SS.reconfigure forSampling @09:43:37.05 SS.seek 25.00ml @09:43:38.40 Vacuum too high -- waiting up to 40s @09:43:38.88 Intake @49.4psia, vacuum from 20.0 to 24.0psi @09:43:38.89 SS.reconfigure forSampling @09:43:38.93 SS.seek 25.00ml @09:43:40.12 Vacuum too high -- waiting up to 40s @09:43:40.82 Intake @49.5psia, vacuum from 20.0 to 24.0psi @09:43:40.83 SS.reconfigure forSampling @09:43:40.87 SS.seek 25.00ml @09:43:41.16 Stroke completed -- waiting for pressure to equalize ... @09:43:44.70 Intake.select :close @09:43:44.85 SSV.dial :exhaust @09:43:45.66 Exhaust.select :open @09:43:45.77 SS.reconfigure SSexhale @09:43:45.81 SS.seek 0.00ml @09:43:54.87 Exhaust.select :close @09:43:54.97 SSV.dial CBV @09:43:54.99 CBV.dial SSV @09:43:55.80 Allowing 185s for 25.0ml of remaining 700.0ml @09:43:55.81 Intake.select :open @09:43:57.95 Intake @49.9psia, vacuum from 20.0 to 24.0psi @09:43:57.96 SS.reconfigure forSampling @09:43:58.00 SS.seek 25.00ml @09:44:00.34 Vacuum too high -- waiting up to 40s @09:44:01.05 Intake @50.2psia, vacuum from 20.0 to 24.0psi @09:44:01.06 SS.reconfigure forSampling @09:44:01.09 SS.seek 25.00ml @09:44:02.43 Vacuum too high -- waiting up to 40s @09:44:03.12 Intake @50.1psia, vacuum from 20.0 to 24.0psi @09:44:03.14 SS.reconfigure forSampling @09:44:03.17 SS.seek 25.00ml @09:44:05.24 Vacuum too high -- waiting up to 40s @09:44:05.51 Intake @50.2psia, vacuum from 20.0 to 24.0psi @09:44:05.52 SS.reconfigure forSampling @09:44:05.55 SS.seek 25.00ml @09:44:07.18 Vacuum too high -- waiting up to 40s @09:44:07.89 Intake @50.3psia, vacuum from 20.0 to 24.0psi @09:44:07.91 SS.reconfigure forSampling @09:44:07.94 SS.seek 25.00ml @09:44:09.28 Vacuum too high -- waiting up to 40s @09:44:09.99 Intake @50.4psia, vacuum from 20.0 to 24.0psi @09:44:10.01 SS.reconfigure forSampling @09:44:10.04 SS.seek 25.00ml @09:44:11.50 Vacuum too high -- waiting up to 40s @09:44:12.21 Intake @50.5psia, vacuum from 20.0 to 24.0psi @09:44:12.23 SS.reconfigure forSampling @09:44:12.26 SS.seek 25.00ml @09:44:13.68 Vacuum too high -- waiting up to 40s @09:44:14.16 Intake @50.1psia, vacuum from 20.0 to 24.0psi @09:44:14.18 SS.reconfigure forSampling @09:44:14.21 SS.seek 25.00ml @09:44:15.37 Vacuum too high -- waiting up to 40s @09:44:16.07 Intake @50.1psia, vacuum from 20.0 to 24.0psi @09:44:16.08 SS.reconfigure forSampling @09:44:16.11 SS.seek 25.00ml @09:44:17.40 Vacuum too high -- waiting up to 40s @09:44:18.11 Intake @49.7psia, vacuum from 20.0 to 24.0psi @09:44:18.12 SS.reconfigure forSampling @09:44:18.16 SS.seek 25.00ml @09:44:19.53 Vacuum too high -- waiting up to 40s @09:44:20.23 Intake @50.6psia, vacuum from 20.0 to 24.0psi @09:44:20.25 SS.reconfigure forSampling @09:44:20.28 SS.seek 25.00ml @09:44:21.56 Vacuum too high -- waiting up to 40s @09:44:22.25 Intake @50.5psia, vacuum from 20.0 to 24.0psi @09:44:22.26 SS.reconfigure forSampling @09:44:22.30 SS.seek 25.00ml @09:44:23.53 Vacuum too high -- waiting up to 40s @09:44:24.01 Intake @50.2psia, vacuum from 20.0 to 24.0psi @09:44:24.02 SS.reconfigure forSampling @09:44:24.06 SS.seek 25.00ml @09:44:25.12 Vacuum too high -- waiting up to 40s @09:44:25.81 Intake @50.0psia, vacuum from 20.0 to 24.0psi @09:44:25.82 SS.reconfigure forSampling @09:44:25.86 SS.seek 25.00ml @09:44:27.25 Vacuum too high -- waiting up to 40s @09:44:27.72 Intake @49.6psia, vacuum from 20.0 to 24.0psi @09:44:27.73 SS.reconfigure forSampling @09:44:27.77 SS.seek 25.00ml @09:44:28.96 Vacuum too high -- waiting up to 40s @09:44:29.64 Intake @50.5psia, vacuum from 20.0 to 24.0psi @09:44:29.65 SS.reconfigure forSampling @09:44:29.69 SS.seek 25.00ml @09:44:30.84 Vacuum too high -- waiting up to 40s @09:44:31.52 Intake @50.8psia, vacuum from 20.0 to 24.0psi @09:44:31.53 SS.reconfigure forSampling @09:44:31.57 SS.seek 25.00ml @09:44:32.84 Vacuum too high -- waiting up to 40s @09:44:33.31 Intake @50.1psia, vacuum from 20.0 to 24.0psi @09:44:33.33 SS.reconfigure forSampling @09:44:33.36 SS.seek 25.00ml @09:44:34.53 Vacuum too high -- waiting up to 40s @09:44:35.23 SS.seek 25.00ml @09:44:37.81 Vacuum too high -- waiting up to 40s @09:44:38.28 Intake @50.0psia, vacuum from 20.0 to 24.0psi @09:44:38.29 SS.reconfigure forSampling @09:44:38.33 SS.seek 25.00ml @09:44:39.34 Vacuum too high -- waiting up to 40s @09:44:40.05 Intake @50.2psia, vacuum from 20.0 to 24.0psi @09:44:40.06 SS.reconfigure forSampling @09:44:40.09 SS.seek 25.00ml @09:44:41.31 Vacuum too high -- waiting up to 40s @09:44:41.80 Intake @49.8psia, vacuum from 20.0 to 24.0psi @09:44:41.82 SS.reconfigure forSampling @09:44:41.85 SS.seek 25.00ml @09:44:42.93 Vacuum too high -- waiting up to 40s @09:44:43.64 Intake @49.6psia, vacuum from 20.0 to 24.0psi @09:44:43.65 SS.reconfigure forSampling @09:44:43.69 SS.seek 25.00ml @09:44:45.06 Vacuum too high -- waiting up to 40s @09:44:45.75 Intake @49.8psia, vacuum from 20.0 to 24.0psi @09:44:45.76 SS.reconfigure forSampling @09:44:45.79 SS.seek 25.00ml @09:44:47.18 Vacuum too high -- waiting up to 40s @09:44:47.86 Intake @50.9psia, vacuum from 20.0 to 24.0psi @09:44:47.87 SS.reconfigure forSampling @09:44:47.91 SS.seek 25.00ml @09:44:48.99 Vacuum too high -- waiting up to 40s @09:44:49.68 Intake @50.6psia, vacuum from 20.0 to 24.0psi @09:44:49.69 SS.reconfigure forSampling @09:44:49.73 SS.seek 25.00ml @09:44:50.93 Vacuum too high -- waiting up to 40s @09:44:51.41 Intake @49.8psia, vacuum from 20.0 to 24.0psi @09:44:51.42 SS.reconfigure forSampling @09:44:51.46 SS.seek 25.00ml @09:44:52.59 Vacuum too high -- waiting up to 40s @09:44:53.28 Intake @50.1psia, vacuum from 20.0 to 24.0psi @09:44:53.29 SS.reconfigure forSampling @09:44:53.32 SS.seek 25.00ml @09:44:54.49 Vacuum too high -- waiting up to 40s @09:44:55.17 Intake @50.2psia, vacuum from 20.0 to 24.0psi @09:44:55.18 SS.reconfigure forSampling @09:44:55.22 SS.seek 25.00ml @09:44:56.31 Vacuum too high -- waiting up to 40s @09:44:56.99 Intake @50.3psia, vacuum from 20.0 to 24.0psi @09:44:57.00 SS.reconfigure forSampling @09:44:57.04 SS.seek 25.00ml @09:44:57.14 delay 1:00 @09:44:58.37 Vacuum too high -- waiting up to 40s @09:44:59.05 Intake @50.6psia, vacuum from 20.0 to 24.0psi @09:44:59.06 SS.reconfigure forSampling @09:44:59.09 SS.seek 25.00ml @09:45:00.27 Vacuum too high -- waiting up to 40s @09:45:00.96 Intake @50.1psia, vacuum from 20.0 to 24.0psi @09:45:00.97 SS.reconfigure forSampling @09:45:01.01 SS.seek 25.00ml @09:45:02.24 Vacuum too high -- waiting up to 40s @09:45:02.93 Intake @49.7psia, vacuum from 20.0 to 24.0psi @09:45:02.94 SS.reconfigure forSampling @09:45:02.97 SS.seek 25.00ml @09:45:04.18 Vacuum too high -- waiting up to 40s @09:45:04.87 Intake @50.3psia, vacuum from 20.0 to 24.0psi @09:45:04.88 SS.reconfigure forSampling @09:45:04.91 SS.seek 25.00ml @09:45:06.15 Vacuum too high -- waiting up to 40s @09:45:07.11 Intake @51.3psia, vacuum from 20.0 to 24.0psi @09:45:07.13 SS.reconfigure forSampling @09:45:07.16 SS.seek 25.00ml @09:45:08.37 Vacuum too high -- waiting up to 40s @09:45:08.86 Intake @50.1psia, vacuum from 20.0 to 24.0psi @09:45:08.88 SS.reconfigure forSampling @09:45:08.91 SS.seek 25.00ml @09:45:10.02 Vacuum too high -- waiting up to 40s @09:45:10.73 Intake @49.9psia, vacuum from 20.0 to 24.0psi @09:45:10.75 SS.reconfigure forSampling @09:45:10.78 SS.seek 25.00ml @09:45:11.99 Vacuum too high -- waiting up to 40s @09:45:12.69 Intake @49.9psia, vacuum from 20.0 to 24.0psi @09:45:12.70 SS.reconfigure forSampling @09:45:12.74 SS.seek 25.00ml @09:45:13.93 Vacuum too high -- waiting up to 40s @09:45:14.62 Intake @49.8psia, vacuum from 20.0 to 24.0psi @09:45:14.63 SS.reconfigure forSampling @09:45:14.67 SS.seek 25.00ml @09:45:15.77 Vacuum too high -- waiting up to 40s @09:45:16.47 Intake @50.2psia, vacuum from 20.0 to 24.0psi @09:45:16.48 SS.reconfigure forSampling @09:45:16.51 SS.seek 25.00ml @09:45:17.56 Vacuum too high -- waiting up to 40s @09:45:18.25 Intake @50.1psia, vacuum from 20.0 to 24.0psi @09:45:18.26 SS.reconfigure forSampling @09:45:18.29 SS.seek 25.00ml @09:45:19.52 Vacuum too high -- waiting up to 40s @09:45:20.21 Intake @50.0psia, vacuum from 20.0 to 24.0psi @09:45:20.22 SS.reconfigure forSampling @09:45:20.26 SS.seek 25.00ml @09:45:21.40 Vacuum too high -- waiting up to 40s @09:45:22.09 Intake @50.2psia, vacuum from 20.0 to 24.0psi @09:45:22.10 SS.reconfigure forSampling @09:45:22.13 SS.seek 25.00ml @09:45:23.18 Vacuum too high -- waiting up to 40s @09:45:23.86 Intake @50.3psia, vacuum from 20.0 to 24.0psi @09:45:23.88 SS.reconfigure forSampling @09:45:23.91 SS.seek 25.00ml @09:45:25.09 Vacuum too high -- waiting up to 40s @09:45:25.80 Intake @50.4psia, vacuum from 20.0 to 24.0psi @09:45:25.81 SS.reconfigure forSampling @09:45:25.85 SS.seek 25.00ml @09:45:26.99 Vacuum too high -- waiting up to 40s @09:45:27.67 Intake @50.9psia, vacuum from 20.0 to 24.0psi @09:45:27.69 SS.reconfigure forSampling @09:45:27.72 SS.seek 25.00ml @09:45:28.77 Vacuum too high -- waiting up to 40s @09:45:29.24 Intake @50.2psia, vacuum from 20.0 to 24.0psi @09:45:29.25 SS.reconfigure forSampling @09:45:29.29 SS.seek 25.00ml @09:45:30.21 Vacuum too high -- waiting up to 40s @09:45:30.89 Intake @49.9psia, vacuum from 20.0 to 24.0psi @09:45:30.91 SS.reconfigure forSampling @09:45:30.94 SS.seek 25.00ml @09:45:32.02 Vacuum too high -- waiting up to 40s @09:45:32.70 Intake @50.0psia, vacuum from 20.0 to 24.0psi @09:45:32.71 SS.reconfigure forSampling @09:45:32.74 SS.seek 25.00ml @09:45:33.87 Vacuum too high -- waiting up to 40s @09:45:35.39 Intake @50.1psia, vacuum from 20.0 to 24.0psi @09:45:35.40 SS.reconfigure forSampling @09:45:35.43 SS.seek 25.00ml @09:45:37.18 Vacuum too high -- waiting up to 40s @09:45:38.53 Intake @50.0psia, vacuum from 20.0 to 24.0psi @09:45:38.54 SS.reconfigure forSampling @09:45:38.58 SS.seek 25.00ml @09:45:40.15 Vacuum too high -- waiting up to 40s @09:45:40.86 Intake @49.7psia, vacuum from 20.0 to 24.0psi @09:45:40.88 SS.reconfigure forSampling @09:45:40.91 SS.seek 25.00ml @09:45:42.05 Vacuum too high -- waiting up to 40s @09:45:42.75 Intake @49.9psia, vacuum from 20.0 to 24.0psi @09:45:42.77 SS.reconfigure forSampling @09:45:42.80 SS.seek 25.00ml @09:45:43.83 Vacuum too high -- waiting up to 40s @09:45:44.52 Intake @50.4psia, vacuum from 20.0 to 24.0psi @09:45:44.53 SS.reconfigure forSampling @09:45:44.57 SS.seek 25.00ml @09:45:45.74 Vacuum too high -- waiting up to 40s @09:45:46.43 Intake @50.8psia, vacuum from 20.0 to 24.0psi @09:45:46.44 SS.reconfigure forSampling @09:45:46.48 SS.seek 25.00ml @09:45:47.52 Vacuum too high -- waiting up to 40s @09:45:48.22 Intake @50.1psia, vacuum from 20.0 to 24.0psi @09:45:48.23 SS.reconfigure forSampling @09:45:48.27 SS.seek 25.00ml @09:45:49.40 Vacuum too high -- waiting up to 40s @09:45:50.08 Intake @49.7psia, vacuum from 20.0 to 24.0psi @09:45:50.10 SS.reconfigure forSampling @09:45:50.13 SS.seek 25.00ml @09:45:51.24 Vacuum too high -- waiting up to 40s @09:45:51.93 Intake @49.7psia, vacuum from 20.0 to 24.0psi @09:45:51.94 SS.reconfigure forSampling @09:45:51.97 SS.seek 25.00ml @09:45:52.99 Vacuum too high -- waiting up to 40s @09:45:53.68 Intake @49.5psia, vacuum from 20.0 to 24.0psi @09:45:53.69 SS.reconfigure forSampling @09:45:53.73 SS.seek 25.00ml @09:45:54.80 Vacuum too high -- waiting up to 40s @09:45:55.49 Intake @49.6psia, vacuum from 20.0 to 24.0psi @09:45:55.50 SS.reconfigure forSampling @09:45:55.54 SS.seek 25.00ml @09:45:56.58 Vacuum too high -- waiting up to 40s @09:45:57.16 AFV.select :carrierSolution @09:45:57.32 Intake @49.5psia, vacuum from 20.0 to 24.0psi @09:45:57.33 SS.reconfigure forSampling @09:45:57.36 delay 2 seconds @09:45:57.38 SS.seek 25.00ml @09:45:58.43 Vacuum too high -- waiting up to 40s @09:45:59.13 SS.seek 25.00ml @09:45:59.37 AS.reconfigure ASconfig @09:45:59.41 AS.seek 1.000ml @09:46:00.15 Vacuum too high -- waiting up to 40s @09:46:00.85 Intake @50.1psia, vacuum from 20.0 to 24.0psi @09:46:00.86 SS.reconfigure forSampling @09:46:00.89 SS.seek 25.00ml @09:46:02.08 Vacuum too high -- waiting up to 40s @09:46:02.78 Intake @50.4psia, vacuum from 20.0 to 24.0psi @09:46:02.79 SS.reconfigure forSampling @09:46:02.82 SS.seek 25.00ml @09:46:03.80 Vacuum too high -- waiting up to 40s @09:46:04.99 Intake @49.8psia, vacuum from 20.0 to 24.0psi @09:46:05.01 SS.reconfigure forSampling @09:46:05.04 SS.seek 25.00ml @09:46:06.40 Vacuum too high -- waiting up to 40s @09:46:07.12 SS.seek 25.00ml @09:46:08.18 Vacuum too high -- waiting up to 40s @09:46:08.89 Intake @49.9psia, vacuum from 20.0 to 24.0psi @09:46:08.91 SS.reconfigure forSampling @09:46:08.94 SS.seek 25.00ml @09:46:10.02 Vacuum too high -- waiting up to 40s @09:46:10.72 Intake @49.9psia, vacuum from 20.0 to 24.0psi @09:46:10.74 SS.reconfigure forSampling @09:46:10.77 SS.seek 25.00ml @09:46:11.83 Vacuum too high -- waiting up to 40s @09:46:12.51 Intake @49.8psia, vacuum from 20.0 to 24.0psi @09:46:12.53 SS.reconfigure forSampling @09:46:12.56 SS.seek 25.00ml @09:46:13.55 Vacuum too high -- waiting up to 40s @09:46:14.24 Intake @49.9psia, vacuum from 20.0 to 24.0psi @09:46:14.25 SS.reconfigure forSampling @09:46:14.29 SS.seek 25.00ml @09:46:15.30 Vacuum too high -- waiting up to 40s @09:46:16.00 Intake @49.8psia, vacuum from 20.0 to 24.0psi @09:46:16.01 SS.reconfigure forSampling @09:46:16.05 SS.seek 25.00ml @09:46:17.15 Vacuum too high -- waiting up to 40s @09:46:17.84 Intake @50.5psia, vacuum from 20.0 to 24.0psi @09:46:17.85 SS.reconfigure forSampling @09:46:17.88 SS.seek 25.00ml @09:46:18.83 Vacuum too high -- waiting up to 40s @09:46:19.52 Intake @50.6psia, vacuum from 20.0 to 24.0psi @09:46:19.53 SS.reconfigure forSampling @09:46:19.56 SS.seek 25.00ml @09:46:20.61 Vacuum too high -- waiting up to 40s @09:46:21.31 Intake @50.5psia, vacuum from 20.0 to 24.0psi @09:46:21.32 SS.reconfigure forSampling @09:46:21.35 SS.seek 25.00ml @09:46:22.33 Vacuum too high -- waiting up to 40s @09:46:23.01 Intake @50.1psia, vacuum from 20.0 to 24.0psi @09:46:23.03 SS.reconfigure forSampling @09:46:23.06 SS.seek 25.00ml @09:46:24.15 Vacuum too high -- waiting up to 40s @09:46:24.83 Intake @50.4psia, vacuum from 20.0 to 24.0psi @09:46:24.84 SS.reconfigure forSampling @09:46:24.88 SS.seek 25.00ml @09:46:25.83 Vacuum too high -- waiting up to 40s @09:46:26.53 Intake @50.2psia, vacuum from 20.0 to 24.0psi @09:46:26.54 SS.reconfigure forSampling @09:46:26.58 SS.seek 25.00ml @09:46:27.52 Vacuum too high -- waiting up to 40s @09:46:28.20 Intake @49.7psia, vacuum from 20.0 to 24.0psi @09:46:28.21 SS.reconfigure forSampling @09:46:28.24 SS.seek 25.00ml @09:46:29.27 Vacuum too high -- waiting up to 40s @09:46:29.97 Intake @49.9psia, vacuum from 20.0 to 24.0psi @09:46:29.98 SS.reconfigure forSampling @09:46:30.03 SS.seek 25.00ml @09:46:31.11 Vacuum too high -- waiting up to 40s @09:46:32.26 Intake @50.0psia, vacuum from 20.0 to 24.0psi @09:46:32.27 SS.reconfigure forSampling @09:46:32.31 SS.seek 25.00ml @09:46:33.52 Vacuum too high -- waiting up to 40s @09:46:34.23 Intake @50.1psia, vacuum from 20.0 to 24.0psi @09:46:34.24 SS.reconfigure forSampling @09:46:34.28 SS.seek 25.00ml @09:46:35.27 Vacuum too high -- waiting up to 40s @09:46:35.97 Intake @50.3psia, vacuum from 20.0 to 24.0psi @09:46:35.99 SS.reconfigure forSampling @09:46:36.02 SS.seek 25.00ml @09:46:37.08 Vacuum too high -- waiting up to 40s @09:46:37.78 Intake @50.7psia, vacuum from 20.0 to 24.0psi @09:46:37.80 SS.reconfigure forSampling @09:46:37.83 SS.seek 25.00ml @09:46:38.83 Vacuum too high -- waiting up to 40s @09:46:39.53 Intake @50.3psia, vacuum from 20.0 to 24.0psi @09:46:39.54 SS.reconfigure forSampling @09:46:39.58 SS.seek 25.00ml @09:46:40.95 Vacuum too high -- waiting up to 40s @09:46:41.22 Intake @49.8psia, vacuum from 20.0 to 24.0psi @09:46:41.23 SS.reconfigure forSampling @09:46:41.27 SS.seek 25.00ml @09:46:42.21 Vacuum too high -- waiting up to 40s @09:46:42.96 Intake @49.9psia, vacuum from 20.0 to 24.0psi @09:46:42.97 SS.reconfigure forSampling @09:46:43.00 SS.seek 25.00ml @09:46:43.99 Vacuum too high -- waiting up to 40s @09:46:44.68 Intake @49.8psia, vacuum from 20.0 to 24.0psi @09:46:44.69 SS.reconfigure forSampling @09:46:44.73 SS.seek 25.00ml @09:46:45.80 Vacuum too high -- waiting up to 40s @09:46:46.48 Intake @49.9psia, vacuum from 20.0 to 24.0psi @09:46:46.49 SS.reconfigure forSampling @09:46:46.52 SS.seek 25.00ml @09:46:47.49 Vacuum too high -- waiting up to 40s @09:46:48.17 Intake @50.1psia, vacuum from 20.0 to 24.0psi @09:46:48.19 SS.reconfigure forSampling @09:46:48.22 SS.seek 25.00ml @09:46:49.14 Vacuum too high -- waiting up to 40s @09:46:49.85 Intake @50.1psia, vacuum from 20.0 to 24.0psi @09:46:49.86 SS.reconfigure forSampling @09:46:49.89 SS.seek 25.00ml @09:46:50.93 Vacuum too high -- waiting up to 40s @09:46:51.62 Intake @50.9psia, vacuum from 20.0 to 24.0psi @09:46:51.63 SS.reconfigure forSampling @09:46:51.67 SS.seek 25.00ml @09:46:52.71 Vacuum too high -- waiting up to 40s @09:46:53.39 Intake @51.0psia, vacuum from 20.0 to 24.0psi @09:46:53.40 SS.reconfigure forSampling @09:46:53.44 SS.seek 25.00ml @09:46:54.36 Vacuum too high -- waiting up to 40s @09:46:55.05 Intake @50.5psia, vacuum from 20.0 to 24.0psi @09:46:55.06 SS.reconfigure forSampling @09:46:55.09 SS.seek 25.00ml @09:46:56.08 Vacuum too high -- waiting up to 40s @09:46:56.77 Intake @50.4psia, vacuum from 20.0 to 24.0psi @09:46:56.78 SS.reconfigure forSampling @09:46:56.81 SS.seek 25.00ml @09:46:57.74 Vacuum too high -- waiting up to 40s @09:46:58.00 delay 1 second @09:46:59.14 Intake @50.2psia, vacuum from 20.0 to 24.0psi @09:46:59.15 SS.reconfigure forSampling @09:46:59.18 AFV.select :holdingCoil @09:46:59.19 SS.seek 25.00ml @09:46:59.32 delay 1 second @09:47:00.33 ATV.dial :ASV @09:47:00.34 ASV.dial :wasteR @09:47:00.35 AS.reconfigure ASslow1 @09:47:00.38 AS.seek 0.000ml @09:47:00.82 SS.reconfigure SSconfig @09:47:00.85 SS.hold @09:47:01.02 Intake.select :close @09:47:01.17 ShallowSampler::Clogged in WCR -- progress too slow after 322.3ml @09:47:01.18 SSV.dial :exhaust @09:47:01.98 Exhaust.select :open @09:47:02.09 SS.reconfigure SSexhale @09:47:02.13 SS.seek 0.00ml @09:47:10.56 Exhaust.select :close @09:47:10.67 Sampled 322.3ml @09:47:10.68 collectSample: Complete @09:47:10.69 Drying puck before fixing for 04:00.00 @09:47:10.70 CBV.dial :waste2b @09:47:11.50 delay 1:00 @09:47:13.66 Sleepy.power :sampler,:OFF @09:48:11.50 CSV.dial :air @09:48:11.96 CS.reconfigure CSfast @09:48:11.99 CS.seek 10.000ml @09:48:36.01 CBV.dial between CSV and waste2b @09:48:36.33 Pumping wcr puck from tube 3 up to 55psi... @09:48:36.37 CBV.dial SSV @09:48:37.81 CBV.dial between CSV and waste2b @09:48:38.76 CSV.dial between air and CTV @09:48:39.16 CS.seek 6.780ml @09:48:47.42 CTV.dial CSV,avoiding => air @09:48:47.44 CSV.dial CTV @09:48:48.19 CS.reconfigure CSpressurize @09:48:48.22 CS.seek 0.000ml @09:49:04.09 CBV.dial :waste2b @09:49:04.41 CS.reconfigure CSconfig @09:49:04.44 CS.seek empty @09:49:07.01 delay 1:00 @09:50:07.02 CTV.dial :air,avoiding => intake @09:50:08.29 Fix #1 of 2 (with rnal) for 02:00.00 @09:50:08.30 CS Pulling 2ml of rnal CSV.dial CSR @09:50:08.72 CSR.select :rnal @09:50:08.82 CS.seek 2.000ml @09:50:14.72 delay 5 seconds @09:50:19.73 CSR.select :air @09:50:19.82 CS.reconfigure CSfast @09:50:19.86 CS.seek 10.000ml @09:50:24.65 delay 20 seconds @09:50:39.13 CBV.dial between CSV and waste2b @09:50:39.46 Pumping wcr puck from tube 3 up to 55psi... @09:50:39.50 CBV.dial SSV @09:50:40.93 CBV.dial between CSV and waste2b @09:50:41.88 CSV.dial between air and CTV @09:50:42.54 CS.seek 6.613ml @09:50:44.66 ATV.dial :air3 @09:50:45.79 AS.seek 0.590ml @09:50:51.23 CTV.dial CSV,avoiding => air @09:50:51.25 CSV.dial CTV @09:50:52.62 CS.reconfigure CSpressurize @09:50:52.65 CS.seek 0.000ml @09:51:09.02 delay 2:00 @09:52:46.45 delay 3 seconds @09:52:49.46 ATV.dial :ASV @09:52:50.54 ASV.dial :wasteR @09:52:50.60 AS.seek 0.090ml @09:53:09.04 CBV.dial :waste2b @09:53:09.36 CS.reconfigure CSconfig @09:53:09.39 CS.seek empty @09:53:11.00 delay 1:00 @09:54:11.01 CTV.dial :air,avoiding => intake @09:54:12.29 Fix #2 of 2 (with rnal) for 02:00.00 @09:54:12.30 CS Pulling 2ml of rnal CSV.dial CSR @09:54:13.20 CSR.select :rnal @09:54:13.30 CS.seek 2.000ml @09:54:19.28 delay 5 seconds @09:54:24.29 CSR.select :air @09:54:24.38 CS.reconfigure CSfast @09:54:24.42 CS.seek 10.000ml @09:54:32.82 delay 30 seconds @09:54:43.69 CBV.dial between CSV and waste2b @09:54:44.03 Pumping wcr puck from tube 3 up to 55psi... @09:54:44.07 CBV.dial SSV @09:54:45.51 CBV.dial between CSV and waste2b @09:54:46.46 CSV.dial between air and CTV @09:54:47.12 CS.seek 6.556ml @09:54:55.85 CTV.dial CSV,avoiding => air @09:54:56.48 CSV.dial CTV @09:54:57.81 CS.reconfigure CSpressurize @09:54:57.84 CS.seek 0.000ml @09:55:02.83 ATV.dial :extSrc @09:55:03.35 delay 3 seconds @09:55:06.36 ATV.dial :ElutionWater @09:55:07.37 AS.seek 0.120ml @09:55:13.76 ATV.dial :air3 @09:55:13.96 delay 2:00 @09:55:14.14 AS.seek 0.130ml @09:55:16.43 delay 2 seconds @09:55:18.44 ATV.dial :bleach @09:55:18.82 AS.seek 0.230ml @09:55:39.48 delay 3 seconds @09:55:42.49 ATV.dial :air3 @09:55:42.83 delay 3 seconds @09:55:45.89 AS.seek 0.255ml @09:55:51.24 delay 3 seconds @09:55:54.25 ATV.dial :bleach @09:55:54.63 AS.seek 0.240ml @09:55:57.93 delay 3 seconds @09:56:00.94 ATV.dial :ASV @09:56:01.83 ASV.dial :SPEdelivery @09:56:02.15 AFV.select :carrierSolution @09:56:02.25 delay 2 seconds @09:56:04.26 AS.reconfigure ASconfig @09:56:04.29 AS.seek 0.500ml @09:56:19.71 delay 1 second @09:56:20.72 AFV.select :holdingCoil @09:56:20.86 AS.reconfigure ASslow1 @09:56:20.89 AS.seek 0.170ml @09:57:13.97 CBV.dial :waste2b @09:57:14.30 CS.reconfigure CSconfig @09:57:14.33 CS.seek empty @09:57:16.06 delay 1:00 @09:57:28.48 SPE.hold 2:46:40,95.0C @09:57:28.51 delay 3:00 @09:58:16.07 CTV.dial :air,avoiding => intake @09:58:17.42 Storing wcr puck in Collection Clamp to tube 2 @09:58:17.55 SC.reconfigure SCtight @09:58:17.58 SC.seek 2 @09:58:19.81 SC.reconfigure SCloose @09:58:19.98 Elbow.reconfigure TightElbow @09:58:20.02 Elbow.seek 2 @09:58:21.61 Forearm.reconfigure TightForearm @09:58:21.65 Forearm.seek 2 + 800 counts @09:58:22.75 SE.reconfigure SEpick @09:58:22.80 SE.seek beamBreak @09:58:25.10 SE.configure SEconfig @09:58:25.22 Elbow.reconfigure LimpElbow @09:58:25.25 Forearm.reconfigure LooseForearm @09:58:25.29 Forearm.seek 2 @09:58:25.51 Elbow.hold @09:58:25.57 Elbow.reconfigure TightElbow @09:58:25.65 SE.seek 6.04 Pucks below Top Plate @09:58:25.99 Forearm.coast @09:58:26.02 Forearm.seek 2 + 332 counts @09:58:26.31 Forearm.reconfigure TightForearm @09:58:26.34 Forearm.seek 2 + 800 counts @09:58:26.52 SE.seek 7.04 Pucks below Top Plate @09:58:27.00 Forearm.reconfigure LooseForearm @09:58:27.03 Forearm.seek retract @09:58:27.95 Elbow.seek CC @09:58:29.55 Elbow.reconfigure LooseElbow @09:58:29.64 Forearm.seek CC @09:58:30.58 CC.seek sealed @09:58:31.52 Hand.grip @09:58:32.20 CC.seek open @09:58:35.54 Forearm.seek retract @09:58:36.48 Elbow.reconfigure TightElbow @09:58:36.52 Elbow.seek 2 @09:58:38.11 Elbow.reconfigure LimpElbow @09:58:38.16 Forearm.seek 2 @09:58:39.12 Elbow.hold @09:58:39.18 Elbow.reconfigure TightElbow @09:58:39.88 Hand.release @09:58:40.66 Forearm.coast @09:58:40.70 Forearm.seek 2 + 335 counts @09:58:40.98 Forearm.reconfigure TightForearm @09:58:41.01 Forearm.seek 2 + 800 counts @09:58:41.23 SE.seek down @09:58:42.33 Forearm.reconfigure LooseForearm @09:58:42.37 Forearm.seek retract @09:58:43.29 SE.coast @09:58:43.33 SC.coast @09:58:43.35 Starting finalPurge @09:58:43.36 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @09:58:43.52 Elbow.seek FlushPuck::Garage @09:58:45.28 Elbow.reconfigure LooseElbow @09:58:45.36 Forearm.seek garage @09:58:46.41 Hand.grip @09:58:47.29 Forearm.seek retract @09:58:48.33 Elbow.reconfigure TightElbow @09:58:48.37 Elbow.seek CC @09:58:51.12 Elbow.reconfigure LooseElbow @09:58:51.20 Forearm.reconfigure TightForearm @09:58:51.23 Forearm.seek CC @09:58:52.34 CC.seek closed @09:58:56.10 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 2.14 @09:58:56.15 Hand.release @09:58:56.87 Forearm.reconfigure LooseForearm @09:58:56.90 Forearm.seek retract @09:58:57.88 Flushing the Collection Valve::Manifold @09:58:57.98 CS Pulling 5ml of flush CSV.dial CSR @09:58:58.43 CSR.select :flush @09:58:58.53 CS.seek 5.000ml @09:59:12.08 delay 5 seconds @09:59:17.09 CSR.select :air @09:59:17.23 CS.reconfigure CSfast @09:59:17.26 CS.seek 7.500ml @09:59:24.11 CSV.dial :waste @09:59:25.42 CS.reconfigure CSconfig @09:59:25.45 CS.seek 0.000ml @09:59:45.30 Flushing lower manifold's waste1 port @09:59:45.39 CSV.dial :flush @09:59:45.84 CS.reconfigure CSfast @09:59:45.87 CS.seek 5.000ml @09:59:58.80 delay 5 seconds @10:00:03.81 CSV.dial :air @10:00:04.30 CS.seek 7.000ml @10:00:09.86 CSV.dial CTV @10:00:09.88
CTV.dial CSV @10:00:11.25 CBV.dial :waste1 @10:00:12.03 CS.seek 0.000ml @10:00:28.53 AFV.select :carrierSolution @10:00:28.62 delay 2 seconds @10:00:29.36
Flushing lower manifold's waste2b port @10:00:29.45 CSV.dial :flush @10:00:30.28 CS.seek 5.000ml @10:00:30.63 AS.reconfigure ASconfig @10:00:30.67 AS.seek 1.000ml @10:00:42.98
delay 5 seconds @10:00:47.99 CSV.dial :air @10:00:48.55 CS.seek 7.000ml @10:00:54.11 CSV.dial CTV @10:00:54.13
CTV.dial CSV @10:00:54.58 CBV.dial :waste2b @10:00:55.34 CS.seek 0.000ml @10:01:12.33 Flushing upper manifold @10:01:12.42 CSV.dial :flush @10:01:13.24 CS.seek 5.000ml @10:01:19.36 delay 1 second @10:01:20.37 AFV.select :holdingCoil @10:01:20.46 delay 1 second @10:01:21.47 ASV.dial :wasteD @10:01:22.49 AS.seek 0.000ml @10:01:25.73
delay 5 seconds @10:01:30.74 CSV.dial :air @10:01:31.30 CS.seek 7.000ml @10:01:36.83 CSV.dial CBV @10:01:36.85
CBV.dial CSV @10:01:38.00 CTV.dial :air @10:01:39.29 CS.seek 0.000ml @10:01:56.45 delay 7 seconds @10:02:03.46 CS.seek 3.000ml @10:02:11.36 CSV.dial :waste @10:02:11.88 CS.seek 0.000ml @10:02:19.69 CSV.dial :flush @10:02:20.13 CS.seek 3.000ml @10:02:21.12 Priming Eluate @10:02:21.13 AFV.select :carrierSolution @10:02:21.22 delay 2 seconds @10:02:23.23 AS.seek 0.500ml @10:02:27.89
delay 5 seconds @10:02:32.90 CSV.dial :air @10:02:33.39 CS.seek 5.000ml @10:02:38.98 CSV.dial :air @10:02:39.00 CS.seek 0.000ml @10:02:51.32 delay 7 seconds @10:02:52.66 delay 1 second @10:02:53.67 AFV.select :holdingCoil @10:02:53.76 ATV.dial ASV @10:02:53.77 ASV.dial :SPErecovery @10:02:54.43 Swish #1 of 2 @10:02:54.48 AS.reconfigure ASslow0 @10:02:54.52 AS.seek 0.600ml @10:02:58.33
CS.seek 3.000ml @10:03:05.04 AS.seek 0.500ml @10:03:06.07
CSV.dial :waste @10:03:06.88 CS.seek 0.000ml @10:03:14.79 Disinfecting external Intake with mfbkill @10:03:14.89 CSV.dial CSR @10:03:15.54 Swish #2 of 2 @10:03:15.60 AS.seek 0.600ml @10:03:16.24
CSR.select :mfbkill @10:03:16.34 CS.seek 8.000ml @10:03:26.15 AS.seek 0.500ml @10:03:35.76
delay 5 seconds @10:03:36.63 delay 3 seconds @10:03:39.69 AS.reconfigure ASslow1 @10:03:39.72 AS.seek 0.676ml @10:03:40.77
CSR.select :air @10:03:40.86 CS.seek 10.000ml @10:03:46.35 CSV.dial CBV @10:03:46.37
CBV.dial CSV @10:03:47.35 CTV.dial :intake @10:03:48.05 Intake.select :open @10:03:48.14 CS.seek 2.000ml @10:04:07.57 Intake.select :close @10:04:07.66 CTV.dial :air @10:04:08.41 CS.reconfigure CSconfig @10:04:08.44 CS.seek 0.000ml @10:04:14.41 Flushing lower manifold's waste1 port @10:04:14.42 CS Pulling 4ml of mfbkill @10:04:14.43 CSV.dial CSR @10:04:15.49 CSR.select :mfbkill @10:04:15.59 CS.seek 4.000ml @10:04:15.88 delay 3 seconds @10:04:18.89 Eluate primed @10:04:18.90 ATV.dial :waste2 @10:04:19.42 AS.reconfigure ASnorm @10:04:19.45 AS.seek 0.000ml @10:04:26.57
delay 5 seconds @10:04:31.58 CSR.select :air @10:04:31.67 CS.reconfigure CSfast @10:04:31.71 CS.seek 10.000ml @10:04:46.38 CSV.dial CTV @10:04:46.40
CTV.dial CSV @10:04:47.76 CBV.dial :waste1 @10:04:48.22 CS.seek 0.000ml @10:04:54.32 SPE.coast @10:04:54.39 ASV.dial :SPErecovery @10:04:54.41 Setting up PCR for HF183em -- writing /var/log/esp/HF183em14mar1310h221ml.pcr @10:04:54.42 Building PCR Slug from ASV with MM2 mix, PP4 primer @10:04:54.43 Priming with carrier @10:04:54.44 ATV.dial ARV @10:04:55.15 ARV.dial :carrier @10:04:55.45 AS.seek 0.500ml @10:05:11.79
Flushing lower manifold's waste2b port @10:05:11.80 CS Pulling 4ml of mfbkill @10:05:11.81 CSV.dial CSR @10:05:12.32 CSR.select :mfbkill @10:05:12.42 CS.reconfigure CSconfig @10:05:12.45 CS.seek 4.000ml @10:05:21.32 AFV.select :carrierSolution @10:05:21.43 delay 2 seconds @10:05:23.44 AS.reconfigure ASconfig @10:05:23.49
delay 5 seconds @10:05:23.51 AS.seek 1.000ml @10:05:28.50
CSR.select :air @10:05:28.59 CS.reconfigure CSfast @10:05:28.63 CS.seek 10.000ml @10:05:43.62 CTV.dial CSV @10:05:43.63 CSV.dial CTV @10:05:44.15
CBV.dial :waste2b @10:05:44.89 CS.seek 0.000ml @10:05:52.91 delay 1 second @10:05:53.92 AFV.select :holdingCoil @10:05:54.02 delay 1 second @10:05:55.03 ATV.dial :waste @10:05:56.29 AS.reconfigure ASnorm @10:05:56.32 AS.seek empty @10:06:08.00
Killing upper manifold @10:06:08.01 CS Pulling 4ml of mfbkill @10:06:08.02 CSV.dial CSR @10:06:08.52 CSR.select :mfbkill @10:06:08.62 CS.reconfigure CSconfig @10:06:08.66 CS.seek 4.000ml @10:06:19.66 delay 5 seconds @10:06:24.68 CSR.select :air @10:06:24.77 CS.reconfigure CSfast @10:06:24.81 CS.seek 10.000ml @10:06:39.22 CSV.dial CBV @10:06:39.24
CBV.dial CSV @10:06:40.28 CTV.dial :air @10:06:41.56 CS.seek 0.000ml @10:06:47.81 AFV.select :carrierSolution @10:06:47.91 delay 2 seconds @10:06:49.92 AS.reconfigure ASconfig @10:06:49.95 AS.seek 0.500ml @10:07:04.72
delay 7 seconds @10:07:11.73 CS.seek 3.000ml @10:07:19.34 CSV.dial :waste @10:07:19.44 delay 1 second @10:07:19.77
CS.seek 0.000ml @10:07:20.45 AFV.select :holdingCoil @10:07:20.76 Building Slug @10:07:20.77 ATV.dial :ARV @10:07:22.03 delay 2 seconds @10:07:24.03 ARV.dial :air1 @10:07:24.38 delay 2 seconds @10:07:26.44 AS.reconfigure ASslow2 @10:07:26.47 AS.seek 0.506ml @10:07:27.37
Flushing upper manifold @10:07:27.46 CSV.dial :flush @10:07:28.12 CS.seek 5.000ml @10:07:32.92 delay 2 seconds @10:07:34.93 ARV.dial :MM2 @10:07:36.23 delay 2 seconds @10:07:38.24 Adding MM2 @10:07:38.29 AS.seek 0.524ml @10:07:40.20
delay 5 seconds @10:07:45.20 CSV.dial :air @10:07:45.77 CS.seek 7.000ml @10:07:51.12 CSV.dial CBV @10:07:51.14
CBV.dial CSV @10:07:52.21 CTV.dial :air @10:07:52.22 CS.seek 0.000ml @10:07:56.86 delay 25 seconds @10:08:08.68
delay 7 seconds @10:08:15.69 CS.seek 3.000ml @10:08:21.87 ARV.dial :PP4 @10:08:22.20 Adding PP4 @10:08:22.22 delay 2 seconds @10:08:23.40
CSV.dial :waste @10:08:23.91 CS.seek 0.000ml @10:08:24.28 AS.seek 0.530ml @10:08:30.51 delay 10 seconds @10:08:31.58
CSV.dial :flush @10:08:32.02 CS.seek 3.000ml @10:08:39.69 delay 5 seconds @10:08:40.52 ARV.dial :air3 @10:08:40.96 adding ARV air @10:08:40.97 delay 2 seconds @10:08:43.02 AS.seek 0.536ml @10:08:44.70
CSV.dial :air @10:08:45.17 CS.seek 5.000ml @10:08:49.37 delay 5 seconds @10:08:50.56
CSV.dial :air @10:08:50.58 CS.seek 0.000ml @10:08:54.39 ARV.dial :carrier @10:08:55.43 delay 7 seconds @10:09:02.49 AS.seek 0.594ml @10:09:02.54
delay 7 seconds @10:09:09.56 CS.seek 3.000ml @10:09:17.19 CSV.dial :waste @10:09:17.98 CS.seek 0.000ml @10:09:25.57 Flushing lower manifold's waste1 port @10:09:25.66 CSV.dial :flush @10:09:26.17 CS.seek 5.000ml @10:09:38.25 delay 5 seconds @10:09:43.26 CSV.dial :air @10:09:43.72 CS.seek 7.000ml @10:09:49.16 CSV.dial CTV @10:09:49.18
CTV.dial CSV @10:09:50.50 CBV.dial :waste1 @10:09:50.96 CS.seek 0.000ml @10:10:02.01 delay 5 seconds @10:10:07.02 ATV.dial :ASV @10:10:07.35
Flushing lower manifold's waste2b port @10:10:08.01 delay 2 seconds @10:10:08.08
CSV.dial :flush @10:10:08.87 CS.seek 5.000ml @10:10:10.07 AS.seek 0.600ml @10:10:16.35 delay 4 seconds @10:10:20.36 ATV.dial ARV @10:10:20.69 delay 2 seconds @10:10:21.00
delay 5 seconds @10:10:22.70 ARV.dial :carrier @10:10:22.71 delay 2 seconds @10:10:24.77 AS.seek 0.650ml @10:10:26.01
CSV.dial :air @10:10:26.57 CS.seek 7.000ml @10:10:32.03 CSV.dial CTV @10:10:32.05
CTV.dial CSV @10:10:32.48 CBV.dial :waste2b @10:10:33.23 CS.seek 0.000ml @10:10:49.66 Disinfecting external Intake with kill @10:10:49.75 CSV.dial CSR @10:10:50.17 CSR.select :kill @10:10:50.27 CS.seek 8.000ml @10:11:09.12 delay 5 seconds @10:11:14.13 CSR.select :air @10:11:14.22 CS.seek 10.000ml @10:11:15.91 delay 4 seconds @10:11:19.62 CSV.dial CBV @10:11:19.64
CBV.dial CSV @10:11:19.92 ATV.dial PCR @10:11:20.44 ASV.dial :waste1 @10:11:20.67
CTV.dial :intake @10:11:21.37 Intake.select :open @10:11:21.42 ARV.dial :air1 @10:11:21.47
CS.seek 2.000ml @10:11:21.76 Positioning Slug by 0.324ml @10:11:21.81 AS.seek 0.326ml @10:11:40.40
Intake.select :close @10:11:40.49 CTV.dial :air @10:11:41.22 CS.reconfigure CSconfig @10:11:41.26 CS.seek 0.000ml @10:11:47.22 CSV.dial CSR @10:11:48.48 CSR.select :flush @10:11:48.58 CS.seek 5.000ml @10:12:02.09 delay 5 seconds @10:12:07.10 CSR.select :air @10:12:07.19 CS.seek 10.000ml @10:12:20.62 CSV.dial CBV @10:12:21.60 CS.seek 0.000ml @10:12:47.74 Garaging FlushPuck in Collection Clamp @10:12:47.80 Elbow.reconfigure TightElbow @10:12:47.83 Elbow.seek CC @10:12:47.93 Elbow.reconfigure LooseElbow @10:12:48.02 Forearm.seek CC @10:12:48.97 CC.seek sealed @10:12:49.90 Hand.grip @10:12:50.68 CC.seek open @10:12:53.99 Forearm.seek retract @10:12:54.93 Elbow.reconfigure TightElbow @10:12:54.97 Elbow.seek garage @10:12:57.77 Elbow.reconfigure LooseElbow @10:12:57.86 Forearm.reconfigure TightForearm @10:12:57.89 Forearm.seek garage @10:12:59.07 Hand.release @10:12:59.84 Forearm.reconfigure LooseForearm @10:12:59.87 Forearm.seek retract @10:16:52.68 delay 5 seconds @10:16:57.69 ATV.dial ARV @10:16:58.23 ASV.dial :PCRlock @10:16:58.90 delay 5 seconds @10:17:03.91 Running HF183em ProfileEM60C2 thermocycling @10:17:03.92 PCR.program ProfileEM60C2 @10:17:04.03 ProfileEM60C2 should complete in < 2:11:38 @10:17:04.04 PCR.run 2:49:32.5 @12:12:24.06 PCR.off @12:12:24.59 PCR for HF183em completed -- closed /var/log/esp/HF183em14mar1310h221ml.pcr @12:12:24.64 Cleaning PCR AFV.select :carrierSolution @12:12:24.73 delay 2 seconds @12:12:26.74 AS.reconfigure ASconfig @12:12:26.77 AS.seek 0.500ml @12:12:37.20 delay 1 second @12:12:38.21 AFV.select :holdingCoil @12:12:38.30 ASV.dial :waste1 @12:12:38.99 ATV.dial :air3 @12:12:39.94 AS.reconfigure ASslow1 @12:12:39.98 AS.seek 0.510ml @12:12:42.26 delay 2 seconds @12:12:44.27 ATV.dial :bleach @12:12:44.67 AS.seek 0.810ml @12:13:46.10 delay 3 seconds @12:13:49.11 ATV.dial :air3 @12:13:49.46 delay 3 seconds @12:13:52.52 AS.seek 0.835ml @12:13:57.85 delay 3 seconds @12:14:00.86 ATV.dial :bleach @12:14:01.26 AS.seek 0.820ml @12:14:04.55 delay 3 seconds @12:14:07.56 ARV.dial :waste @12:14:08.16 delay 3 seconds @12:14:11.17 ATV.dial :ARV @12:14:11.89 AS.reconfigure ASconfig @12:14:11.93 AS.seek 0.470ml @12:14:32.60 AFV.select :carrierSolution @12:14:32.69 delay 2 seconds @12:14:34.70 AS.seek 1.000ml @12:15:05.91 delay 1 second @12:15:06.92 AFV.select :holdingCoil @12:15:07.01 delay 1 second @12:15:08.02 AS.reconfigure ASfast @12:15:08.05 AS.seek 0.000ml @12:15:35.78 AFV.select :carrierSolution @12:15:35.87 delay 2 seconds @12:15:37.88 AS.reconfigure ASconfig @12:15:37.91 AS.seek 1.000ml @12:16:36.52 delay 1 second @12:16:37.53 AFV.select :holdingCoil @12:16:37.62 delay 1 second @12:16:38.63 AS.reconfigure ASfast @12:16:38.66 AS.seek 0.000ml @12:17:06.39 AFV.select :carrierSolution @12:17:06.48 delay 2 seconds @12:17:08.49 AS.reconfigure ASconfig @12:17:08.52 AS.seek 1.000ml @12:18:07.14 delay 1 second @12:18:08.15 AFV.select :holdingCoil @12:18:08.24 delay 1 second @12:18:09.25 AS.reconfigure ASfast @12:18:09.28 AS.seek 0.000ml @12:18:37.01 ARV.dial between carrier and air1 @12:18:37.51 ATV.dial :air3 @12:18:38.43 AS.reconfigure ASslow1 @12:18:38.47 AS.seek 0.010ml @12:18:40.84 delay 2 seconds @12:18:42.85 ATV.dial :bleach @12:18:43.23 AS.seek 0.810ml @12:21:26.60 delay 3 seconds @12:21:29.61 ATV.dial :air3 @12:21:29.96 delay 3 seconds @12:21:33.02 AS.seek 0.835ml @12:21:38.33 delay 3 seconds @12:21:41.34 ATV.dial :bleach @12:21:41.73 AS.seek 0.820ml @12:21:45.05 delay 3 seconds @12:21:48.06 ATV.dial PCR @12:21:48.38 AS.reconfigure ASconfig @12:21:48.42 AS.seek empty @12:22:36.55 PCR.program :bleachProfile @12:22:36.60 PCR program should complete in < 2:45 @12:22:36.61 PCR.run 10:00 @12:25:35.45 PCR.off @12:25:35.48 AFV.select :carrierSolution @12:25:35.57 delay 2 seconds @12:25:37.58 AS.seek 1.000ml @12:26:36.22 delay 1 second @12:26:37.23 AFV.select :holdingCoil @12:26:37.32 delay 1 second @12:26:38.33 AS.reconfigure ASfast @12:26:38.36 AS.seek 0.000ml @12:27:06.07 delay 3 seconds @12:27:09.08 AFV.select :carrierSolution @12:27:09.17 delay 2 seconds @12:27:11.18 AS.reconfigure ASconfig @12:27:11.21 AS.seek 1.000ml @12:28:09.82 delay 1 second @12:28:10.83 AFV.select :holdingCoil @12:28:10.92 delay 1 second @12:28:11.93 AS.reconfigure ASfast @12:28:11.96 AS.seek 0.000ml @12:28:39.66 delay 3 seconds @12:28:42.67 AFV.select :carrierSolution @12:28:42.76 delay 2 seconds @12:28:44.77 AS.reconfigure ASconfig @12:28:44.80 AS.seek 1.000ml @12:29:43.37 delay 1 second @12:29:44.38 AFV.select :holdingCoil @12:29:44.47 delay 1 second @12:29:45.48 AS.reconfigure ASfast @12:29:45.61 AS.seek 0.000ml @12:30:13.34 delay 3 seconds @12:30:16.35 AFV.select :carrierSolution @12:30:16.44 delay 2 seconds @12:30:18.45 AS.reconfigure ASconfig @12:30:18.48 AS.seek 1.000ml @12:31:17.07 delay 1 second @12:31:18.08 AFV.select :holdingCoil @12:31:18.17 delay 1 second @12:31:19.18 AS.reconfigure ASfast @12:31:19.21 AS.seek 0.000ml @12:31:46.95 delay 3 seconds @12:31:49.96 AFV.select :carrierSolution @12:31:50.05 delay 2 seconds @12:31:52.06 AS.reconfigure ASconfig @12:31:52.09 AS.seek 1.000ml @12:32:50.68 delay 1 second @12:32:51.69 AFV.select :holdingCoil @12:32:51.78 delay 1 second @12:32:52.79 AS.reconfigure ASnorm @12:32:52.82 AS.seek 0.000ml @12:33:44.29 Setting up PCR for ENTap -- writing /var/log/esp/ENTap14mar1312h221ml.pcr @12:33:44.30 Building PCR Slug from ASV with MM1 mix, PP1 primer @12:33:44.31 Priming with carrier @12:33:44.32 ATV.dial ARV @12:33:44.91 ARV.dial :carrier @12:33:45.18 AS.seek 0.500ml @12:34:11.05 AFV.select :carrierSolution @12:34:11.14 delay 2 seconds @12:34:13.15 AS.reconfigure ASconfig @12:34:13.19 AS.seek 1.000ml @12:34:42.63 delay 1 second @12:34:43.64 AFV.select :holdingCoil @12:34:43.73 delay 1 second @12:34:44.74 ATV.dial :waste @12:34:46.00 AS.reconfigure ASnorm @12:34:46.03 AS.seek empty @12:35:37.52 AFV.select :carrierSolution @12:35:37.62 delay 2 seconds @12:35:39.63 AS.reconfigure ASconfig @12:35:39.67 AS.seek 0.500ml @12:36:09.16 delay 1 second @12:36:10.17 AFV.select :holdingCoil @12:36:10.26 Building Slug @12:36:10.27 ATV.dial :ARV @12:36:11.52 delay 2 seconds @12:36:13.53 ARV.dial :air1 @12:36:13.86 delay 2 seconds @12:36:15.92 AS.reconfigure ASslow2 @12:36:15.95 AS.seek 0.506ml @12:36:22.22 delay 2 seconds @12:36:24.23 ARV.dial :MM1 @12:36:24.72 delay 2 seconds @12:36:26.73 Adding MM1 @12:36:26.78 AS.seek 0.524ml @12:36:45.33 delay 25 seconds @12:37:10.34 ARV.dial :PP1 @12:37:10.67 Adding PP1 @12:37:10.68 delay 2 seconds @12:37:12.74 AS.seek 0.530ml @12:37:18.97 delay 10 seconds @12:37:28.98 ARV.dial :air1 @12:37:29.30 adding ARV air @12:37:29.31 delay 2 seconds @12:37:31.37 AS.seek 0.536ml @12:37:37.86 delay 5 seconds @12:37:42.87 ARV.dial :carrier @12:37:43.19 delay 7 seconds @12:37:50.25 AS.seek 0.594ml @12:38:49.92 delay 5 seconds @12:38:54.93 ATV.dial :ASV @12:38:55.25 delay 2 seconds @12:38:57.31 AS.seek 0.600ml @12:39:03.67 delay 4 seconds @12:39:07.68 ATV.dial ARV @12:39:08.01 delay 2 seconds @12:39:10.02 ARV.dial :carrier @12:39:10.03 delay 2 seconds @12:39:12.08 AS.seek 0.650ml @12:40:03.25 delay 4 seconds @12:40:07.26 ATV.dial PCR @12:40:07.76 ASV.dial :waste1 @12:40:07.77 ARV.dial :air1 @12:40:08.10 Positioning Slug by 0.324ml @12:40:08.16 AS.seek 0.326ml @12:45:38.87 delay 5 seconds @12:45:43.88 ATV.dial ARV @12:45:44.40 ASV.dial :PCRlock @12:45:44.84 delay 5 seconds @12:45:49.85 Running ENTap Profile60C thermocycling @12:45:49.86 PCR.program Profile60C @12:45:49.96 Profile60C should complete in < 1:39:08 @12:45:49.97 PCR.run 2:08:55 @14:08:54.56 PCR.off @14:08:55.08 PCR for ENTap completed -- closed /var/log/esp/ENTap14mar1312h221ml.pcr @14:08:55.13 Cleaning PCR AFV.select :carrierSolution @14:08:55.22 delay 2 seconds @14:08:57.23 AS.reconfigure ASconfig @14:08:57.26 AS.seek 0.500ml @14:09:07.68 delay 1 second @14:09:08.69 AFV.select :holdingCoil @14:09:08.78 ASV.dial :waste1 @14:09:09.24 ATV.dial :air3 @14:09:10.17 AS.reconfigure ASslow1 @14:09:10.21 AS.seek 0.510ml @14:09:12.50 delay 2 seconds @14:09:14.51 ATV.dial :bleach @14:09:14.88 AS.seek 0.810ml @14:10:16.31 delay 3 seconds @14:10:19.32 ATV.dial :air3 @14:10:19.66 delay 3 seconds @14:10:22.72 AS.seek 0.835ml @14:10:28.05 delay 3 seconds @14:10:31.06 ATV.dial :bleach @14:10:31.46 AS.seek 0.820ml @14:10:34.74 delay 3 seconds @14:10:37.75 ARV.dial :waste @14:10:38.25 delay 3 seconds @14:10:41.26 ATV.dial :ARV @14:10:41.98 AS.reconfigure ASconfig @14:10:42.02 AS.seek 0.470ml @14:11:02.69 AFV.select :carrierSolution @14:11:02.78 delay 2 seconds @14:11:04.79 AS.seek 1.000ml @14:11:35.97 delay 1 second @14:11:36.98 AFV.select :holdingCoil @14:11:37.07 delay 1 second @14:11:38.08 AS.reconfigure ASfast @14:11:38.11 AS.seek 0.000ml @14:12:05.84 AFV.select :carrierSolution @14:12:05.93 delay 2 seconds @14:12:07.94 AS.reconfigure ASconfig @14:12:07.97 AS.seek 1.000ml @14:13:06.54 delay 1 second @14:13:07.55 AFV.select :holdingCoil @14:13:07.64 delay 1 second @14:13:08.65 AS.reconfigure ASfast @14:13:08.68 AS.seek 0.000ml @14:13:36.42 AFV.select :carrierSolution @14:13:36.51 delay 2 seconds @14:13:38.52 AS.reconfigure ASconfig @14:13:38.55 AS.seek 1.000ml @14:14:37.15 delay 1 second @14:14:38.16 AFV.select :holdingCoil @14:14:38.25 delay 1 second @14:14:39.26 AS.reconfigure ASfast @14:14:39.29 AS.seek 0.000ml @14:15:07.02 ARV.dial between carrier and air1 @14:15:07.43 ATV.dial :air3 @14:15:08.36 AS.reconfigure ASslow1 @14:15:08.40 AS.seek 0.010ml @14:15:10.73 delay 2 seconds @14:15:12.74 ATV.dial :bleach @14:15:13.78 AS.seek 0.810ml @14:17:57.21 delay 3 seconds @14:18:00.22 ATV.dial :air3 @14:18:00.57 delay 3 seconds @14:18:03.63 AS.seek 0.835ml @14:18:08.94 delay 3 seconds @14:18:11.95 ATV.dial :bleach @14:18:12.35 AS.seek 0.820ml @14:18:15.66 delay 3 seconds @14:18:18.67 ATV.dial PCR @14:18:19.00 AS.reconfigure ASconfig @14:18:19.03 AS.seek empty @14:19:07.14 PCR.program :bleachProfile @14:19:07.19 PCR program should complete in < 2:45 @14:19:07.20 PCR.run 10:00 @14:22:05.39 PCR.off @14:22:05.42 AFV.select :carrierSolution @14:22:05.51 delay 2 seconds @14:22:07.52 AS.seek 1.000ml @14:23:06.16 delay 1 second @14:23:07.17 AFV.select :holdingCoil @14:23:07.26 delay 1 second @14:23:08.27 AS.reconfigure ASfast @14:23:08.30 AS.seek 0.000ml @14:23:36.05 delay 3 seconds @14:23:39.06 AFV.select :carrierSolution @14:23:39.15 delay 2 seconds @14:23:41.16 AS.reconfigure ASconfig @14:23:41.19 AS.seek 1.000ml @14:24:39.80 delay 1 second @14:24:40.81 AFV.select :holdingCoil @14:24:40.90 delay 1 second @14:24:41.91 AS.reconfigure ASfast @14:24:41.94 AS.seek 0.000ml @14:25:09.69 delay 3 seconds @14:25:12.70 AFV.select :carrierSolution @14:25:12.79 delay 2 seconds @14:25:14.80 AS.reconfigure ASconfig @14:25:14.83 AS.seek 1.000ml @14:26:13.43 delay 1 second @14:26:14.44 AFV.select :holdingCoil @14:26:14.53 delay 1 second @14:26:15.54 AS.reconfigure ASfast @14:26:15.68 AS.seek 0.000ml @14:26:43.43 delay 3 seconds @14:26:46.44 AFV.select :carrierSolution @14:26:46.53 delay 2 seconds @14:26:48.54 AS.reconfigure ASconfig @14:26:48.57 AS.seek 1.000ml @14:27:47.13 delay 1 second @14:27:48.14 AFV.select :holdingCoil @14:27:48.23 delay 1 second @14:27:49.24 AS.reconfigure ASfast @14:27:49.27 AS.seek 0.000ml @14:28:17.01 delay 3 seconds @14:28:20.02 AFV.select :carrierSolution @14:28:20.11 delay 2 seconds @14:28:22.12 AS.reconfigure ASconfig @14:28:22.15 AS.seek 1.000ml @14:29:20.72 delay 1 second @14:29:21.73 AFV.select :holdingCoil @14:29:21.82 delay 1 second @14:29:22.83 AS.reconfigure ASnorm @14:29:22.86 AS.seek 0.000ml @14:30:14.33 Setting up PCR for ENTem -- writing /var/log/esp/ENTem14mar1314h221ml.pcr @14:30:14.34 Building PCR Slug from ASV with MM2 mix, PP3 primer @14:30:14.35 Priming with carrier @14:30:14.36 ATV.dial ARV @14:30:14.88 ARV.dial :carrier @14:30:15.16 AS.seek 0.500ml @14:30:41.03 AFV.select :carrierSolution @14:30:41.12 delay 2 seconds @14:30:43.13 AS.reconfigure ASconfig @14:30:43.16 AS.seek 1.000ml @14:31:12.60 delay 1 second @14:31:13.61 AFV.select :holdingCoil @14:31:13.70 delay 1 second @14:31:14.71 ATV.dial :waste @14:31:16.06 AS.reconfigure ASnorm @14:31:16.09 AS.seek empty @14:32:07.58 AFV.select :carrierSolution @14:32:07.67 delay 2 seconds @14:32:09.68 AS.reconfigure ASconfig @14:32:09.71 AS.seek 0.500ml @14:32:39.22 delay 1 second @14:32:40.23 AFV.select :holdingCoil @14:32:40.32 Building Slug @14:32:40.33 ATV.dial :ARV @14:32:41.58 delay 2 seconds @14:32:43.59 ARV.dial :air1 @14:32:43.92 delay 2 seconds @14:32:45.98 AS.reconfigure ASslow2 @14:32:46.01 AS.seek 0.506ml @14:32:52.36 delay 2 seconds @14:32:54.37 ARV.dial :MM2 @14:32:55.59 delay 2 seconds @14:32:57.60 Adding MM2 @14:32:57.66 AS.seek 0.524ml @14:33:16.15 delay 25 seconds @14:33:41.16 ARV.dial :PP3 @14:33:41.57 Adding PP3 @14:33:41.58 delay 2 seconds @14:33:43.63 AS.seek 0.530ml @14:33:49.97 delay 10 seconds @14:33:59.98 ARV.dial :air2 @14:34:00.40 adding ARV air @14:34:00.41 delay 2 seconds @14:34:02.46 AS.seek 0.536ml @14:34:09.01 delay 5 seconds @14:34:14.02 ARV.dial :carrier @14:34:15.04 delay 7 seconds @14:34:22.10 AS.seek 0.594ml @14:35:21.60 delay 5 seconds @14:35:26.61 ATV.dial :ASV @14:35:27.02 delay 2 seconds @14:35:29.08 AS.seek 0.600ml @14:35:35.46 delay 4 seconds @14:35:39.47 ATV.dial ARV @14:35:39.77 delay 2 seconds @14:35:41.78 ARV.dial :carrier @14:35:41.79 delay 2 seconds @14:35:43.84 AS.seek 0.650ml @14:36:35.32 delay 4 seconds @14:36:39.33 ATV.dial PCR @14:36:40.43 ASV.dial :waste1 @14:36:40.44 ARV.dial :air1 @14:36:40.78 Positioning Slug by 0.324ml @14:36:40.83 AS.seek 0.326ml @14:42:11.37 delay 5 seconds @14:42:16.38 ATV.dial ARV @14:42:16.90 ASV.dial :PCRlock @14:42:17.40 delay 5 seconds @14:42:22.41 Running ENTem ProfileEM60C thermocycling @14:42:22.42 PCR.program ProfileEM60C @14:42:22.53 ProfileEM60C should complete in < 2:11:38 @14:42:22.54 PCR.run 2:49:32.5 @16:38:21.45 PCR.off @16:38:21.98 PCR for ENTem completed -- closed /var/log/esp/ENTem14mar1314h221ml.pcr @16:38:22.03 Cleaning PCR @16:38:22.04 AFV.select :carrierSolution @16:38:22.13 delay 2 seconds @16:38:24.14 AS.reconfigure ASconfig @16:38:24.17 AS.seek 0.500ml @16:38:34.60 delay 1 second @16:38:35.61 AFV.select :holdingCoil @16:38:35.70 ASV.dial :waste1 @16:38:36.18 ATV.dial :air3 @16:38:37.11 AS.reconfigure ASslow1 @16:38:37.15 AS.seek 0.510ml @16:38:39.44 delay 2 seconds @16:38:41.45 ATV.dial :bleach @16:38:41.82 AS.seek 0.810ml @16:39:43.24 delay 3 seconds @16:39:46.25 ATV.dial :air3 @16:39:46.68 delay 3 seconds @16:39:49.74 AS.seek 0.835ml @16:39:55.08 delay 3 seconds @16:39:58.09 ATV.dial :bleach @16:39:58.46 AS.seek 0.820ml @16:40:01.75 delay 3 seconds @16:40:04.76 ARV.dial :waste @16:40:05.25 delay 3 seconds @16:40:08.26 ATV.dial :ARV @16:40:08.98 AS.reconfigure ASconfig @16:40:09.02 AS.seek 0.470ml @16:40:29.71 AFV.select :carrierSolution @16:40:29.80 delay 2 seconds @16:40:31.81 AS.seek 1.000ml @16:41:03.00 delay 1 second @16:41:04.01 AFV.select :holdingCoil @16:41:04.10 delay 1 second @16:41:05.11 AS.reconfigure ASfast @16:41:05.14 AS.seek 0.000ml @16:41:32.89 AFV.select :carrierSolution @16:41:32.98 delay 2 seconds @16:41:34.99 AS.reconfigure ASconfig @16:41:35.02 AS.seek 1.000ml @16:42:33.60 delay 1 second @16:42:34.61 AFV.select :holdingCoil @16:42:35.35 delay 1 second @16:42:36.36 AS.reconfigure ASfast @16:42:36.39 AS.seek 0.000ml @16:43:04.13 AFV.select :carrierSolution @16:43:04.23 delay 2 seconds @16:43:06.24 AS.reconfigure ASconfig @16:43:06.27 AS.seek 1.000ml @16:44:04.87 delay 1 second @16:44:05.88 AFV.select :holdingCoil @16:44:05.97 delay 1 second @16:44:06.98 AS.reconfigure ASfast @16:44:07.01 AS.seek 0.000ml @16:44:34.72 ARV.dial between carrier and air1 @16:44:35.13 ATV.dial :air3 @16:44:36.15 AS.reconfigure ASslow1 @16:44:36.18 AS.seek 0.010ml @16:44:38.51 delay 2 seconds @16:44:40.52 ATV.dial :bleach @16:44:41.01 AS.seek 0.810ml @16:47:24.37 delay 3 seconds @16:47:27.38 ATV.dial :air3 @16:47:27.70 delay 3 seconds @16:47:30.76 AS.seek 0.835ml @16:47:36.09 delay 3 seconds @16:47:39.10 ATV.dial :bleach @16:47:39.49 AS.seek 0.820ml @16:47:42.80 delay 3 seconds @16:47:45.81 ATV.dial PCR @16:47:46.12 AS.reconfigure ASconfig @16:47:46.16 AS.seek empty @16:48:34.28 PCR.program :bleachProfile @16:48:34.33 PCR program should complete in < 2:45 @16:48:34.34 PCR.run 10:00 @16:51:32.53 PCR.off @16:51:32.56 AFV.select :carrierSolution @16:51:32.65 delay 2 seconds @16:51:34.66 AS.seek 1.000ml @16:52:33.27 delay 1 second @16:52:34.28 AFV.select :holdingCoil @16:52:34.37 delay 1 second @16:52:35.38 AS.reconfigure ASfast @16:52:35.41 AS.seek 0.000ml @16:53:03.15 delay 3 seconds @16:53:06.16 AFV.select :carrierSolution @16:53:06.25 delay 2 seconds @16:53:08.26 AS.reconfigure ASconfig @16:53:08.29 AS.seek 1.000ml @16:54:06.86 delay 1 second @16:54:07.87 AFV.select :holdingCoil @16:54:07.96 delay 1 second @16:54:08.97 AS.reconfigure ASfast @16:54:09.00 AS.seek 0.000ml @16:54:36.72 delay 3 seconds @16:54:39.73 AFV.select :carrierSolution @16:54:39.82 delay 2 seconds @16:54:41.83 AS.reconfigure ASconfig @16:54:41.86 AS.seek 1.000ml @16:55:40.44 delay 1 second @16:55:41.45 AFV.select :holdingCoil @16:55:41.54 delay 1 second @16:55:42.55 AS.reconfigure ASfast @16:55:42.59 AS.seek 0.000ml @16:56:10.33 delay 3 seconds @16:56:13.34 AFV.select :carrierSolution @16:56:13.43 delay 2 seconds @16:56:15.44 AS.reconfigure ASconfig @16:56:15.47 AS.seek 1.000ml @16:57:14.07 delay 1 second @16:57:15.08 AFV.select :holdingCoil @16:57:15.17 delay 1 second @16:57:16.18 AS.reconfigure ASfast @16:57:16.21 AS.seek 0.000ml @16:57:43.95 delay 3 seconds @16:57:46.96 AFV.select :carrierSolution @16:57:47.05 delay 2 seconds @16:57:49.06 AS.reconfigure ASconfig @16:57:49.09 AS.seek 1.000ml @16:58:47.67 delay 1 second @16:58:48.68 AFV.select :holdingCoil @16:58:48.77 delay 1 second @16:58:49.78 AS.reconfigure ASnorm @16:58:49.81 AS.seek 0.000ml @16:59:41.28 Setting up PCR for IPCap -- writing /var/log/esp/IPCap14mar1316h221ml.pcr @16:59:41.29 Building PCR Slug from ASV with MM1 mix, PP2 primer @16:59:41.30 Priming with carrier @16:59:41.31 ATV.dial ARV @16:59:41.83 ARV.dial :carrier @16:59:42.11 AS.seek 0.500ml @17:00:07.98 AFV.select :carrierSolution @17:00:08.07 delay 2 seconds @17:00:10.08 AS.reconfigure ASconfig @17:00:10.11 AS.seek 1.000ml @17:00:39.53 delay 1 second @17:00:40.54 AFV.select :holdingCoil @17:00:40.63 delay 1 second @17:00:41.64 ATV.dial :waste @17:00:42.99 AS.reconfigure ASnorm @17:00:43.02 AS.seek empty @17:01:34.48 AFV.select :carrierSolution @17:01:34.57 delay 2 seconds @17:01:36.58 AS.reconfigure ASconfig @17:01:36.61 AS.seek 0.500ml @17:02:06.09 delay 1 second @17:02:07.10 AFV.select :holdingCoil @17:02:07.19 Building Slug @17:02:07.20 ATV.dial :ARV @17:02:08.45 delay 2 seconds @17:02:10.46 ARV.dial :air1 @17:02:10.79 delay 2 seconds @17:02:12.85 AS.reconfigure ASslow2 @17:02:12.88 AS.seek 0.506ml @17:02:19.34 delay 2 seconds @17:02:21.35 ARV.dial :MM1 @17:02:21.93 delay 2 seconds @17:02:23.94 Adding MM1 @17:02:23.99 AS.seek 0.524ml @17:02:42.68 delay 25 seconds @17:03:07.69 ARV.dial :PP2 @17:03:08.01 Adding PP2 @17:03:08.02 delay 2 seconds @17:03:10.08 AS.seek 0.530ml @17:03:16.39 delay 10 seconds @17:03:26.40 ARV.dial :air2 @17:03:26.82 adding ARV air @17:03:26.83 delay 2 seconds @17:03:28.89 AS.seek 0.536ml @17:03:36.15 delay 5 seconds @17:03:41.16 ARV.dial :carrier @17:03:42.18 delay 7 seconds @17:03:49.24 AS.seek 0.594ml @17:04:48.84 delay 5 seconds @17:04:53.85 ATV.dial :ASV @17:04:54.27 delay 2 seconds @17:04:56.33 AS.seek 0.600ml @17:05:02.79 delay 4 seconds @17:05:06.80 ATV.dial ARV @17:05:07.12 delay 2 seconds @17:05:09.13 ARV.dial :carrier @17:05:09.14 delay 2 seconds @17:05:11.19 AS.seek 0.650ml @17:06:02.35 delay 4 seconds @17:06:06.36 ATV.dial PCR @17:06:06.86 ASV.dial :waste1 @17:06:06.87 ARV.dial :air1 @17:06:07.21 Positioning Slug by 0.324ml @17:06:07.27 AS.seek 0.326ml @17:11:38.10 delay 5 seconds @17:11:43.11 ATV.dial ARV @17:11:43.62 ASV.dial :PCRlock @17:11:44.10 delay 5 seconds @17:11:49.11 Running IPCap Profile59C thermocycling @17:11:49.12 PCR.program Profile59C @17:11:49.22 Profile59C should complete in < 1:39:08 @17:11:49.23 PCR.run 2:08:55 @18:35:28.19 PCR.off @18:35:28.72 PCR for IPCap completed -- closed /var/log/esp/IPCap14mar1316h221ml.pcr @18:35:28.77 Cleaning PCR @18:35:28.78 AFV.select :carrierSolution @18:35:28.87 delay 2 seconds @18:35:30.88 AS.reconfigure ASconfig @18:35:30.95 AS.seek 0.500ml @18:35:41.33 delay 1 second @18:35:42.34 AFV.select :holdingCoil @18:35:42.43 ASV.dial :waste1 @18:35:42.91 ATV.dial :air3 @18:35:43.84 AS.reconfigure ASslow1 @18:35:43.88 AS.seek 0.510ml @18:35:46.18 delay 2 seconds @18:35:48.19 ATV.dial :bleach @18:35:48.57 AS.seek 0.810ml @18:36:50.00 delay 3 seconds @18:36:53.01 ATV.dial :air3 @18:36:53.42 delay 3 seconds @18:36:56.48 AS.seek 0.835ml @18:37:01.81 delay 3 seconds @18:37:05.46 ATV.dial :bleach @18:37:05.83 AS.seek 0.820ml @18:37:09.14 delay 3 seconds @18:37:12.15 ARV.dial :waste @18:37:12.74 delay 3 seconds @18:37:15.75 ATV.dial :ARV @18:37:16.50 AS.reconfigure ASconfig @18:37:16.53 AS.seek 0.470ml @18:37:37.22 AFV.select :carrierSolution @18:37:37.31 delay 2 seconds @18:37:39.32 AS.seek 1.000ml @18:38:10.51 delay 1 second @18:38:11.52 AFV.select :holdingCoil @18:38:11.61 delay 1 second @18:38:12.62 AS.reconfigure ASfast @18:38:12.66 AS.seek 0.000ml @18:38:40.37 AFV.select :carrierSolution @18:38:40.47 delay 2 seconds @18:38:42.48 AS.reconfigure ASconfig @18:38:42.51 AS.seek 1.000ml @18:39:41.09 delay 1 second @18:39:42.10 AFV.select :holdingCoil @18:39:42.19 delay 1 second @18:39:43.20 AS.reconfigure ASfast @18:39:43.23 AS.seek 0.000ml @18:40:10.95 AFV.select :carrierSolution @18:40:11.05 delay 2 seconds @18:40:13.06 AS.reconfigure ASconfig @18:40:13.09 AS.seek 1.000ml @18:41:11.71 delay 1 second @18:41:12.72 AFV.select :holdingCoil @18:41:12.81 delay 1 second @18:41:13.82 AS.reconfigure ASfast @18:41:13.85 AS.seek 0.000ml @18:41:41.56 ARV.dial between carrier and air1 @18:41:41.97 ATV.dial :air3 @18:41:42.90 AS.reconfigure ASslow1 @18:41:42.94 AS.seek 0.010ml @18:41:45.28 delay 2 seconds @18:41:47.29 ATV.dial :bleach @18:41:47.77 AS.seek 0.810ml @18:44:31.14 delay 3 seconds @18:44:34.15 ATV.dial :air3 @18:44:34.48 delay 3 seconds @18:44:37.54 AS.seek 0.835ml @18:44:42.90 delay 3 seconds @18:44:45.91 ATV.dial :bleach @18:44:46.30 AS.seek 0.820ml @18:44:49.62 delay 3 seconds @18:44:52.63 ATV.dial PCR @18:44:52.93 AS.reconfigure ASconfig @18:44:52.97 AS.seek empty @18:45:41.09 PCR.program :bleachProfile @18:45:41.14 PCR program should complete in < 2:45 @18:45:41.15 PCR.run 10:00 @18:48:42.36 PCR.off @18:48:42.39 AFV.select :carrierSolution @18:48:42.48 delay 2 seconds @18:48:44.49 AS.seek 1.000ml @18:49:43.13 delay 1 second @18:49:44.14 AFV.select :holdingCoil @18:49:44.23 delay 1 second @18:49:45.24 AS.reconfigure ASfast @18:49:45.27 AS.seek 0.000ml @18:50:12.99 delay 3 seconds @18:50:16.00 AFV.select :carrierSolution @18:50:16.09 delay 2 seconds @18:50:18.10 AS.reconfigure ASconfig @18:50:18.13 AS.seek 1.000ml @18:51:16.72 delay 1 second @18:51:17.73 AFV.select :holdingCoil @18:51:17.82 delay 1 second @18:51:18.83 AS.reconfigure ASfast @18:51:18.86 AS.seek 0.000ml @18:51:46.59 delay 3 seconds @18:51:49.60 AFV.select :carrierSolution @18:51:49.69 delay 2 seconds @18:51:51.70 AS.reconfigure ASconfig @18:51:51.73 AS.seek 1.000ml @18:52:50.32 delay 1 second @18:52:51.33 AFV.select :holdingCoil @18:52:51.42 delay 1 second @18:52:52.43 AS.reconfigure ASfast @18:52:52.46 AS.seek 0.000ml @18:53:20.20 delay 3 seconds @18:53:23.21 AFV.select :carrierSolution @18:53:23.30 delay 2 seconds @18:53:25.31 AS.reconfigure ASconfig @18:53:25.34 AS.seek 1.000ml @18:54:23.93 delay 1 second @18:54:24.94 AFV.select :holdingCoil @18:54:25.03 delay 1 second @18:54:26.04 AS.reconfigure ASfast @18:54:26.07 AS.seek 0.000ml @18:54:53.82 delay 3 seconds @18:54:56.83 AFV.select :carrierSolution @18:54:56.92 delay 2 seconds @18:54:58.93 AS.reconfigure ASconfig @18:54:58.96 AS.seek 1.000ml @18:55:57.54 delay 1 second @18:55:58.55 AFV.select :holdingCoil @18:55:58.64 delay 1 second @18:55:59.65 AS.reconfigure ASnorm @18:55:59.68 AS.seek 0.000ml @18:56:51.11 Setting up PCR for IPCem -- writing /var/log/esp/IPCem14mar1318h221ml.pcr @18:56:51.12 Building PCR Slug from ASV with MM3 mix, PP5 primer @18:56:51.13 Priming with carrier @18:56:51.14 ATV.dial ARV @18:56:51.64 ARV.dial :carrier @18:56:51.93 AS.seek 0.500ml @18:57:17.80 AFV.select :carrierSolution @18:57:17.89 delay 2 seconds @18:57:19.90 AS.reconfigure ASconfig @18:57:19.93 AS.seek 1.000ml @18:57:49.37 delay 1 second @18:57:50.38 AFV.select :holdingCoil @18:57:50.47 delay 1 second @18:57:51.48 ATV.dial :waste @18:57:52.74 AS.reconfigure ASnorm @18:57:52.77 AS.seek empty @18:58:44.26 AFV.select :carrierSolution @18:58:44.35 delay 2 seconds @18:58:46.36 AS.reconfigure ASconfig @18:58:46.39 AS.seek 0.500ml @18:59:15.90 delay 1 second @18:59:16.91 AFV.select :holdingCoil @18:59:17.00 Building Slug @18:59:17.01 ATV.dial :ARV @18:59:18.26 delay 2 seconds @18:59:20.27 ARV.dial :air1 @18:59:20.90 delay 2 seconds @18:59:22.96 AS.reconfigure ASslow2 @18:59:22.99 AS.seek 0.506ml @18:59:29.48 delay 2 seconds @18:59:31.49 ARV.dial :MM3 @18:59:32.35 delay 2 seconds @18:59:34.36 Adding MM3 @18:59:34.41 AS.seek 0.524ml @18:59:53.07 delay 25 seconds @19:00:18.08 ARV.dial :PP5 @19:00:18.51 Adding PP5 @19:00:18.52 delay 2 seconds @19:00:20.58 AS.seek 0.530ml @19:00:27.02 delay 10 seconds @19:00:37.03 ARV.dial :air3 @19:00:37.37 adding ARV air @19:00:37.38 delay 2 seconds @19:00:39.44 AS.seek 0.536ml @19:00:45.74 delay 5 seconds @19:00:50.75 ARV.dial :carrier @19:00:51.79 delay 7 seconds @19:00:58.85 AS.seek 0.594ml @19:01:58.30 delay 5 seconds @19:02:03.31 ATV.dial :ASV @19:02:03.64 delay 2 seconds @19:02:05.70 AS.seek 0.600ml @19:02:12.13 delay 4 seconds @19:02:16.14 ATV.dial ARV @19:02:16.47 delay 2 seconds @19:02:18.48 ARV.dial :carrier @19:02:18.49 delay 2 seconds @19:02:20.54 AS.seek 0.650ml @19:03:11.89 delay 4 seconds @19:03:15.90 ATV.dial PCR @19:03:16.39 ASV.dial :waste1 @19:03:16.40 ARV.dial :air1 @19:03:16.75 Positioning Slug by 0.324ml @19:03:16.80 AS.seek 0.326ml @19:08:47.29 delay 5 seconds @19:08:52.30 ATV.dial ARV @19:08:52.82 ASV.dial :PCRlock @19:08:53.30 delay 5 seconds @19:08:58.31 Running IPCem ProfileEM59C thermocycling @19:08:58.32 PCR.program ProfileEM59C @19:08:58.43 ProfileEM59C should complete in < 2:11:38 @19:08:58.44 PCR.run 2:49:32.5 @21:04:40.76 PCR.off @21:04:41.29 PCR for IPCem completed -- closed /var/log/esp/IPCem14mar1318h221ml.pcr @21:04:41.31 Full MFB Decontamination Decon extraction column and decon AIV @21:04:41.95 SPE.hold 2:46:40,55.0C @21:04:41.97 ASV.dial :SPEdelivery @21:04:42.65 delay 20 seconds @21:05:02.66 AFV.select :carrierSolution @21:05:02.75 delay 2 seconds @21:05:04.76 AS.reconfigure ASconfig @21:05:04.79 AS.seek 0.500ml @21:05:15.20 delay 1 second @21:05:16.21 AFV.select :holdingCoil @21:05:16.30 ATV.dial :air1 @21:05:17.91 AS.reconfigure ASslow1 @21:05:17.94 AS.seek 0.520ml @21:05:22.28 ATV.dial :ASV @21:05:23.56 ASV.dial :SPErecovery @21:05:24.39 AS.seek 0.620ml @21:05:45.03 ATV.dial :waste @21:05:46.47 AS.reconfigure ASnorm @21:05:46.50 AS.seek empty @21:06:18.56 AFV.select :holdingCoil @21:06:18.57 ATV.dial :waste @21:06:18.58 AS.reconfigure ASconfig @21:06:18.61 AS.seek 0.000ml @21:06:18.92 AFV.select :carrierSolution @21:06:19.02 delay 2 seconds @21:06:21.03 AS.seek 0.500ml @21:06:50.43 delay 1 second @21:06:51.44 AFV.select :holdingCoil @21:06:51.53 ATV.dial :air3 @21:06:52.09 AS.reconfigure ASslow1 @21:06:52.12 AS.seek 0.520ml @21:06:56.45 ATV.dial :air3 @21:06:56.51 AS.seek 0.530ml @21:06:58.77 delay 2 seconds @21:07:00.78 ATV.dial :bleach @21:07:01.15 AS.seek 0.710ml @21:07:38.10 delay 3 seconds @21:07:41.11 ATV.dial :air3 @21:07:41.45 delay 3 seconds @21:07:44.51 AS.seek 0.735ml @21:07:49.85 delay 3 seconds @21:07:52.86 ATV.dial :bleach @21:07:53.26 AS.seek 0.720ml @21:07:56.54 delay 3 seconds @21:07:59.55 ATV.dial ASV @21:08:00.44 ASV.dial :SPErecovery @21:08:00.49 AS.seek 0.365ml @21:09:13.13 ATV.dial :waste @21:09:14.59 AS.reconfigure ASnorm @21:09:14.62 AS.seek empty @21:09:33.66 ATV.dial :air3 @21:09:34.20 AS.reconfigure ASslow1 @21:09:34.24 AS.seek 0.009ml @21:09:36.58 delay 2 seconds @21:09:38.59 ATV.dial :bleach @21:09:38.96 AS.seek 0.579ml @21:11:35.37 delay 3 seconds @21:11:38.38 ATV.dial :air3 @21:11:38.72 delay 3 seconds @21:11:41.78 AS.seek 0.604ml @21:11:47.14 delay 3 seconds @21:11:50.15 ATV.dial :bleach @21:11:50.55 AS.seek 0.589ml @21:11:53.84 delay 3 seconds @21:11:56.85 ATV.dial :ASV @21:11:57.83 ASV.dial :wasteR @21:11:58.43 AS.seek 0.000ml @21:13:58.84 delay 1:00 @21:14:58.86 ATV.dial ASV @21:14:58.87 AFV.select :carrierSolution @21:14:58.96 delay 2 seconds @21:15:00.97 AS.reconfigure ASconfig @21:15:01.00 AS.seek 1.000ml @21:15:59.60 delay 1 second @21:16:00.61 AFV.select :holdingCoil @21:16:00.70 delay 1 second @21:16:01.71 ASV.dial :wasteR @21:16:01.72 AS.reconfigure ASslow1 @21:16:01.75 AS.seek 0.000ml @21:19:26.01 delay 10 seconds @21:19:36.02 ATV.dial :air3 @21:19:37.14 AS.seek 0.010ml @21:19:39.48 delay 2 seconds @21:19:41.49 ATV.dial :bleach @21:19:41.89 AS.seek 0.580ml @21:21:38.40 delay 3 seconds @21:21:41.41 ATV.dial :air3 @21:21:41.76 delay 3 seconds @21:21:44.82 AS.seek 0.605ml @21:21:50.16 delay 3 seconds @21:21:53.17 ATV.dial :bleach @21:21:53.65 AS.seek 0.590ml @21:21:56.94 delay 3 seconds @21:21:59.95 ATV.dial ASV @21:22:00.83 ASV.dial :SPEdelivery @21:22:01.31 AS.seek 0.000ml @21:24:01.86 delay 1:00 @21:25:01.87 AFV.select :carrierSolution @21:25:01.96 delay 2 seconds @21:25:03.97 AS.reconfigure ASconfig @21:25:04.00 AS.seek 1.000ml @21:26:02.59 delay 1 second @21:26:03.60 AFV.select :holdingCoil @21:26:03.69 delay 1 second @21:26:04.70 ATV.dial ASV @21:26:04.71 ASV.dial :SPEdelivery @21:26:04.72 AS.reconfigure ASslow1 @21:26:04.75 AS.seek 0.000ml @21:29:28.99 delay 10 seconds @21:29:39.00 ATV.dial :air2 @21:29:39.70 AS.reconfigure ASslow0 @21:29:39.73 AS.seek 0.800ml @21:31:01.68 delay 3 seconds @21:31:04.69 ATV.dial :ASV @21:31:05.51 ASV.dial :SPEdelivery @21:31:05.53 AS.reconfigure ASslow1 @21:31:05.56 AS.seek 0.030ml @21:33:42.85 delay 20 seconds @21:34:02.86 ATV.dial :waste,via => air3 @21:34:04.32 AS.reconfigure ASconfig @21:34:04.35 AS.seek empty @21:34:06.45 SPE.coast @21:34:06.53 Removing Bleach from Mixing Coil AFV.select :carrierSolution @21:34:07.23 delay 2 seconds @21:34:09.24 AS.seek 0.500ml @21:34:38.73 delay 1 second @21:34:39.74 AFV.select :holdingCoil @21:34:39.83 ATV.dial :ASV @21:34:41.27 ASV.dial :SPErecovery @21:34:41.95 AS.reconfigure ASslow1 @21:34:41.99 AS.seek 0.730ml @21:35:29.18 ASV.dial :wasteD @21:35:29.69 AS.reconfigure ASnorm @21:35:29.72 AS.seek 0.000ml @21:36:07.35 AFV.select :carrierSolution @21:36:07.44 delay 2 seconds @21:36:09.45 AS.reconfigure ASconfig @21:36:09.48 AS.seek 0.500ml @21:36:38.93 delay 1 second @21:36:39.94 AFV.select :holdingCoil @21:36:40.03 ATV.dial ASV @21:36:40.04 ASV.dial :SPErecovery @21:36:40.73 AS.reconfigure ASslow0 @21:36:40.77 AS.seek 0.300ml @21:37:01.46 ATV.dial :air2 @21:37:02.21 AS.reconfigure ASslow1 @21:37:02.24 AS.seek 0.320ml @21:37:06.58 ATV.dial ASV @21:37:07.35 AS.reconfigure ASslow0 @21:37:07.38 AS.seek 0.570ml @21:37:33.13 ASV.dial :wasteD @21:37:33.79 AS.reconfigure ASnorm @21:37:33.82 AS.seek 0.000ml @21:38:03.28 AFV.select :carrierSolution @21:38:03.37 delay 2 seconds @21:38:05.38 AS.reconfigure ASconfig @21:38:05.41 AS.seek 0.500ml @21:38:34.84 delay 1 second @21:38:35.85 AFV.select :holdingCoil @21:38:35.99 ATV.dial ASV @21:38:36.00 ASV.dial :SPErecovery @21:38:36.70 AS.reconfigure ASslow0 @21:38:36.74 AS.seek 0.300ml @21:38:57.45 ATV.dial :air2 @21:38:58.21 AS.reconfigure ASslow1 @21:38:58.25 AS.seek 0.320ml @21:39:02.62 ATV.dial ASV @21:39:03.48 AS.reconfigure ASslow0 @21:39:03.52 AS.seek 0.570ml @21:39:29.34 ASV.dial :wasteD @21:39:30.01 AS.reconfigure ASnorm @21:39:30.04 AS.seek 0.000ml @21:39:59.51 ATV.dial :air2 @21:40:00.32 AS.reconfigure ASslow0 @21:40:00.35 AS.seek 0.300ml @21:40:31.23 ATV.dial :ASV @21:40:32.03 ASV.dial :wasteD @21:40:32.05 AS.reconfigure ASconfig @21:40:32.08 AS.seek 0.050ml @21:40:46.95 ATV.dial :waste @21:40:48.40 AS.seek 0.000ml @21:40:51.57 ASV.dial :SPEdelivery @21:40:52.59 deconLysateINT @21:40:52.60 ATV.dial :air3 @21:40:53.16 AS.reconfigure ASslow1 @21:40:53.20 AS.seek 0.010ml @21:40:55.46 delay 2 seconds @21:40:57.47 ATV.dial :bleach @21:40:57.84 AS.seek 0.960ml @21:44:11.93 delay 3 seconds @21:44:14.94 ATV.dial :air3 @21:44:15.29 delay 3 seconds @21:44:18.35 AS.seek 0.985ml @21:44:23.74 delay 3 seconds @21:44:26.75 ATV.dial :bleach @21:44:27.15 AS.seek 0.970ml @21:44:30.44 delay 3 seconds @21:44:33.45 ATV.dial :AIV @21:44:34.50 AIV.dial :coilToMFB @21:44:34.51 AWV.select :waste @21:44:34.52 AFV.select :carrierSolution @21:44:34.61 delay 2 seconds @21:44:36.62 AS.reconfigure ASconfig @21:44:36.65 AS.seek 1.000ml @21:44:38.74 delay 1 second @21:44:39.75 AFV.select :holdingCoil @21:44:39.84 delay 1 second @21:44:40.85 AS.reconfigure ASslow0 @21:44:40.88 AS.seek empty @21:46:23.27 AFV.select :carrierSolution @21:46:23.36 delay 2 seconds @21:46:25.37 AS.reconfigure ASconfig @21:46:25.40 AS.seek 1.000ml @21:47:24.05 delay 1 second @21:47:25.06 AFV.select :holdingCoil @21:47:25.15 delay 1 second @21:47:26.16 AS.reconfigure ASslow0 @21:47:26.19 AS.seek empty @21:49:08.60 AFV.select :carrierSolution @21:49:08.69 delay 2 seconds @21:49:10.70 AS.reconfigure ASconfig @21:49:10.73 AS.seek 1.000ml @21:50:09.38 delay 1 second @21:50:10.39 AFV.select :holdingCoil @21:50:10.48 delay 1 second @21:50:11.49 AS.reconfigure ASslow0 @21:50:11.52 AS.seek empty @21:51:53.94 ATV.dial :air2 @21:51:54.51 AS.reconfigure ASslow1 @21:51:54.55 AS.seek 0.009ml @21:51:56.88 AS.reconfigure ASslow0 @21:51:56.92 AS.seek 1.000ml @21:53:38.25 ATV.dial AIV @21:53:38.79 AS.seek 0.020ml @21:55:19.07 ATV.dial :waste @21:55:20.35 AS.seek 0.000ml @21:55:22.67 AWV.select :air @21:55:22.81 Cleaning PCR @21:55:22.82 AFV.select :carrierSolution @21:55:22.91 delay 2 seconds @21:55:24.92 AS.reconfigure ASconfig @21:55:24.95 AS.seek 0.500ml @21:55:54.40 delay 1 second @21:55:55.41 AFV.select :holdingCoil @21:55:55.50 ASV.dial :waste1 @21:55:56.04 ATV.dial :air3 @21:55:57.20 AS.reconfigure ASslow1 @21:55:57.24 AS.seek 0.510ml @21:55:59.57 delay 2 seconds @21:56:01.58 ATV.dial :bleach @21:56:01.95 AS.seek 0.810ml @21:57:03.43 delay 3 seconds @21:57:06.44 ATV.dial :air3 @21:57:06.88 delay 3 seconds @21:57:09.94 AS.seek 0.835ml @21:57:15.35 delay 3 seconds @21:57:18.36 ATV.dial :bleach @21:57:18.74 AS.seek 0.820ml @21:57:22.02 delay 3 seconds @21:57:25.03 ARV.dial :waste @21:57:25.52 delay 3 seconds @21:57:28.53 ATV.dial :ARV @21:57:29.26 AS.reconfigure ASconfig @21:57:29.30 AS.seek 0.470ml @21:57:49.97 AFV.select :carrierSolution @21:57:50.06 delay 2 seconds @21:57:52.07 AS.seek 1.000ml @21:58:23.25 delay 1 second @21:58:24.26 AFV.select :holdingCoil @21:58:24.35 delay 1 second @21:58:25.36 AS.reconfigure ASfast @21:58:25.39 AS.seek 0.000ml @21:58:53.13 AFV.select :carrierSolution @21:58:53.23 delay 2 seconds @21:58:55.24 AS.reconfigure ASconfig @21:58:55.27 AS.seek 1.000ml @21:59:53.84 delay 1 second @21:59:54.85 AFV.select :holdingCoil @21:59:54.94 delay 1 second @21:59:55.95 AS.reconfigure ASfast @21:59:55.98 AS.seek 0.000ml @22:00:23.72 AFV.select :carrierSolution @22:00:23.82 delay 2 seconds @22:00:25.83 AS.reconfigure ASconfig @22:00:25.86 AS.seek 1.000ml @22:01:24.45 delay 1 second @22:01:25.46 AFV.select :holdingCoil @22:01:25.55 delay 1 second @22:01:26.56 AS.reconfigure ASfast @22:01:26.59 AS.seek 0.000ml @22:01:54.33 ARV.dial between carrier and air1 @22:01:54.75 ATV.dial :air3 @22:01:55.69 AS.reconfigure ASslow1 @22:01:55.73 AS.seek 0.010ml @22:01:58.07 delay 2 seconds @22:02:00.08 ATV.dial :bleach @22:02:00.56 AS.seek 0.810ml @22:04:43.96 delay 3 seconds @22:04:46.97 ATV.dial :air3 @22:04:47.30 delay 3 seconds @22:04:50.36 AS.seek 0.835ml @22:04:55.69 delay 3 seconds @22:04:58.70 ATV.dial :bleach @22:04:59.09 AS.seek 0.820ml @22:05:02.40 delay 3 seconds @22:05:05.41 ATV.dial PCR @22:05:05.74 AS.reconfigure ASconfig @22:05:05.77 AS.seek empty @22:05:54.08 PCR.program :bleachProfile @22:05:54.14 PCR program should complete in < 2:45 @22:05:54.15 PCR.run 10:00 @22:08:58.38 PCR.off @22:08:58.41 AFV.select :carrierSolution @22:08:58.50 delay 2 seconds @22:09:00.51 AS.seek 1.000ml @22:09:59.13 delay 1 second @22:10:00.14 AFV.select :holdingCoil @22:10:00.23 delay 1 second @22:10:01.24 AS.reconfigure ASfast @22:10:01.27 AS.seek 0.000ml @22:10:29.01 delay 3 seconds @22:10:32.02 AFV.select :carrierSolution @22:10:32.11 delay 2 seconds @22:10:34.12 AS.reconfigure ASconfig @22:10:34.15 AS.seek 1.000ml @22:11:32.74 delay 1 second @22:11:33.75 AFV.select :holdingCoil @22:11:33.84 delay 1 second @22:11:34.85 AS.reconfigure ASfast @22:11:34.88 AS.seek 0.000ml @22:12:02.63 delay 3 seconds @22:12:05.64 AFV.select :carrierSolution @22:12:05.73 delay 2 seconds @22:12:07.74 AS.reconfigure ASconfig @22:12:07.77 AS.seek 1.000ml @22:13:06.35 delay 1 second @22:13:07.36 AFV.select :holdingCoil @22:13:07.45 delay 1 second @22:13:08.46 AS.reconfigure ASfast @22:13:08.49 AS.seek 0.000ml @22:13:36.23 delay 3 seconds @22:13:39.24 AFV.select :carrierSolution @22:13:39.33 delay 2 seconds @22:13:41.34 AS.reconfigure ASconfig @22:13:41.37 AS.seek 1.000ml @22:14:39.97 delay 1 second @22:14:40.98 AFV.select :holdingCoil @22:14:41.07 delay 1 second @22:14:42.08 AS.reconfigure ASfast @22:14:42.11 AS.seek 0.000ml @22:15:09.86 delay 3 seconds @22:15:12.87 AFV.select :carrierSolution @22:15:12.96 delay 2 seconds @22:15:14.97 AS.reconfigure ASconfig @22:15:15.00 AS.seek 1.000ml @22:16:13.60 delay 1 second @22:16:14.61 AFV.select :holdingCoil @22:16:14.70 delay 1 second @22:16:15.71 AS.reconfigure ASnorm @22:16:15.74 AS.seek 0.000ml @22:17:07.20 AFV.select :carrierSolution @22:17:07.30 delay 2 seconds @22:17:09.31 AS.reconfigure ASconfig @22:17:09.34 AS.seek 1.000ml @22:18:07.91 delay 1 second @22:18:08.92 AFV.select :holdingCoil @22:18:09.01 delay 1 second @22:18:10.02 ATV.dial :waste @22:18:10.93 AS.reconfigure ASnorm @22:18:10.97 AS.seek 0.500ml @22:18:36.88 ATV.dial :waste2 @22:18:37.97 AS.seek 0.000ml @22:19:04.43
Cleaning PCR @22:19:04.44 AFV.select :carrierSolution @22:19:04.53 delay 2 seconds @22:19:06.54 AS.reconfigure ASconfig @22:19:06.57 AS.seek 0.500ml @22:19:36.00 delay 1 second @22:19:37.01 AFV.select :holdingCoil @22:19:37.10 ASV.dial :waste1 @22:19:37.11 ATV.dial :air3 @22:19:38.69 AS.reconfigure ASslow1 @22:19:38.73 AS.seek 0.510ml @22:19:41.02 delay 2 seconds @22:19:43.03 ATV.dial :bleach @22:19:43.41 AS.seek 0.810ml @22:20:44.83 delay 3 seconds @22:20:47.84 ATV.dial :air3 @22:20:48.17 delay 3 seconds @22:20:51.23 AS.seek 0.835ml @22:20:56.55 delay 3 seconds @22:20:59.56 ATV.dial :bleach @22:21:00.05 AS.seek 0.820ml @22:21:03.36 delay 3 seconds @22:21:06.37 ARV.dial :waste @22:21:06.77 delay 3 seconds @22:21:09.78 ATV.dial :ARV @22:21:10.65 AS.reconfigure ASconfig @22:21:10.68 AS.seek 0.470ml @22:21:31.36 AFV.select :carrierSolution @22:21:31.45 delay 2 seconds @22:21:33.46 AS.seek 1.000ml @22:22:04.67 delay 1 second @22:22:05.68 AFV.select :holdingCoil @22:22:05.77 delay 1 second @22:22:06.78 AS.reconfigure ASfast @22:22:06.81 AS.seek 0.000ml @22:22:34.56 AFV.select :carrierSolution @22:22:34.65 delay 2 seconds @22:22:36.66 AS.reconfigure ASconfig @22:22:36.69 AS.seek 1.000ml @22:23:35.30 delay 1 second @22:23:36.31 AFV.select :holdingCoil @22:23:36.40 delay 1 second @22:23:37.41 AS.reconfigure ASfast @22:23:37.44 AS.seek 0.000ml @22:24:05.19 AFV.select :carrierSolution @22:24:05.28 delay 2 seconds @22:24:07.29 AS.reconfigure ASconfig @22:24:07.32 AS.seek 1.000ml @22:25:05.91 delay 1 second @22:25:06.92 AFV.select :holdingCoil @22:25:07.01 delay 1 second @22:25:08.02 AS.reconfigure ASfast @22:25:08.05 AS.seek 0.000ml @22:25:35.75 ARV.dial between carrier and air1 @22:25:36.16 ATV.dial :air3 @22:25:37.07 AS.reconfigure ASslow1 @22:25:37.11 AS.seek 0.010ml @22:25:39.43 delay 2 seconds @22:25:41.44 ATV.dial :bleach @22:25:41.93 AS.seek 0.810ml @22:28:25.33 delay 3 seconds @22:28:28.34 ATV.dial :air3 @22:28:28.67 delay 3 seconds @22:28:31.73 AS.seek 0.835ml @22:28:37.15 delay 3 seconds @22:28:40.16 ATV.dial :bleach @22:28:40.55 AS.seek 0.820ml @22:28:43.95 delay 3 seconds @22:28:46.96 ATV.dial PCR @22:28:47.29 AS.reconfigure ASconfig @22:28:47.32 AS.seek empty @22:29:35.46 PCR.program :bleachProfile @22:29:35.51 PCR program should complete in < 2:45 @22:29:35.52 PCR.run 10:00 @22:32:39.75 PCR.off @22:32:39.78 AFV.select :carrierSolution @22:32:39.87 delay 2 seconds @22:32:41.88 AS.seek 1.000ml @22:33:40.53 delay 1 second @22:33:41.54 AFV.select :holdingCoil @22:33:41.63 delay 1 second @22:33:42.64 AS.reconfigure ASfast @22:33:42.67 AS.seek 0.000ml @22:34:10.39 delay 3 seconds @22:34:13.40 AFV.select :carrierSolution @22:34:13.49 delay 2 seconds @22:34:15.50 AS.reconfigure ASconfig @22:34:15.53 AS.seek 1.000ml @22:35:14.11 delay 1 second @22:35:15.12 AFV.select :holdingCoil @22:35:15.21 delay 1 second @22:35:16.22 AS.reconfigure ASfast @22:35:16.25 AS.seek 0.000ml @22:35:43.98 delay 3 seconds @22:35:46.99 AFV.select :carrierSolution @22:35:47.08 delay 2 seconds @22:35:49.09 AS.reconfigure ASconfig @22:35:49.12 AS.seek 1.000ml @22:36:47.70 delay 1 second @22:36:48.71 AFV.select :holdingCoil @22:36:48.80 delay 1 second @22:36:49.81 AS.reconfigure ASfast @22:36:49.84 AS.seek 0.000ml @22:37:17.56 delay 3 seconds @22:37:20.57 AFV.select :carrierSolution @22:37:20.66 delay 2 seconds @22:37:22.67 AS.reconfigure ASconfig @22:37:22.70 AS.seek 1.000ml @22:38:21.31 delay 1 second @22:38:22.32 AFV.select :holdingCoil @22:38:22.41 delay 1 second @22:38:23.42 AS.reconfigure ASfast @22:38:23.45 AS.seek 0.000ml @22:38:51.20 delay 3 seconds @22:38:54.21 AFV.select :carrierSolution @22:38:54.30 delay 2 seconds @22:38:56.31 AS.reconfigure ASconfig @22:38:56.34 AS.seek 1.000ml @22:39:54.94 delay 1 second @22:39:55.95 AFV.select :holdingCoil @22:39:56.04 delay 1 second @22:39:57.05 AS.reconfigure ASnorm @22:39:57.08 AS.seek 0.000ml @22:40:48.54 Completed {wqPCR} begun 08:01:37 @22:40:48.57 Asleep til 08:01PDT14-Mar-14 @22:40:49.14 AS.stop @22:40:49.15
Sleeping until 08:01PDT14-Mar-14 @22:40:49.17 GoodNews.email "Then, in tube 3: wqPCR\n/home/esp/esp2local/mission/14mar05sccwrpDeploy.rb:64",Subject => "Asleep until 08:01PDT14-Mar-14" @22:40:50.85 CC.stop @22:40:50.88 CS.stop @22:40:50.90 PC.stop @22:40:50.93 PS.stop @22:40:50.96 Forearm.stop @22:40:50.99 Elbow.stop @22:40:51.02 SS.stop @22:40:51.09 Sleepy.power :raw,:OFF @22:40:51.10 Sleepy.power :dwarf,:OFF Shutdown @22:48:21.46 Session Begun @22:49:53.34 -> abort! "Need to power down for pump wiring repair" @22:49:53.35 CmdServer::Client::Error in Blythe -- Aborting Mission: Need to power down for pump wiring repair @22:49:53.38 BadNews.email "Need to power down for pump wiring repair",Subject => "14mar05sccwrpDeploy ABORTED!" @22:50:02.16 Session Ended @13:26:46.71PDT14-Mar-14 ESP2 v3.20 I2C Gateway v3.01 @13:26:47.05 Powering up Dwarves @13:26:53.65
Configuration for neo by maro@mbari.org /home/esp/esp2/shallow/neo/configure.rb @13:26:53.74 Configuration for MFB10 by preston@mbari.org /home/esp/esp2/mfb/MFB10/configure.rb @13:26:57.01 Sleepy.power :raw,:ON @13:26:57.72 SPE.coast @13:26:57.76 SPE.configure SPEconfig @13:26:57.79 AS.stop @13:26:57.82 AS.configure ASconfig @13:26:57.85 CC.stop @13:26:57.87 CC.configure CCconfig @13:26:57.90 CS.stop @13:26:57.92 CS.configure CSconfig @13:26:57.95 PC.stop @13:26:57.97 PC.configure PCconfig @13:26:58.00 PS.stop @13:26:58.02 PS.configure PSconfig @13:26:58.08 Forearm.stop @13:26:58.10 Forearm.configure LooseForearm @13:26:58.13 Elbow.stop @13:26:58.15 Elbow.configure LooseElbow @13:26:58.21 SC.stop @13:26:58.23 SC.configure SCloose @13:26:58.26 SE.stop @13:26:58.29 SE.configure SEconfig @13:26:58.32 SS.stop @13:26:58.34 SS.configure SSconfig @13:26:58.37 CH.coast @13:26:58.41 CH.configure CHconfig @13:26:58.44 PH.coast @13:26:58.48 PH.configure PHconfig @13:26:58.52 PCR.off @13:26:58.55 PCR.pidtemp= 50,0.1,0,300,25,25 @13:26:58.58 PCR.caltemp= 6.6661,0.0344,0.0 @13:26:58.61 All dwarves are running firmware version 3.74 @13:26:59.16 GoodNews.email "Starting w/pucks in tube 3\n ",Subject => "14mar14sccwrp begun" @13:27:00.16
Asleep til +0:10 @13:27:00.20 Sleeping until 13:27 @13:27:00.24 Sleepy.power :raw,:OFF @13:27:00.25 Sleepy.power :dwarf,:OFF Shutdown @13:27:08.66 GoodNews.email "Then, in tube 3: wqPCR\n/home/esp/esp2/mission/14mar14sccwrp.rb:55",Subject => "Asleep until 13:27" @13:27:09.64
Powering up Dwarves @13:27:14.55 Sleepy.power :raw,:ON @13:27:15.04 SPE.configure SPEconfig @13:27:15.07 AS.configure ASconfig @13:27:15.10 CC.configure CCconfig @13:27:15.13 CS.configure CSconfig @13:27:15.16 PC.configure PCconfig @13:27:15.19 PS.configure PSconfig @13:27:15.22 Forearm.configure LooseForearm @13:27:15.25 Elbow.configure LooseElbow @13:27:15.28 SC.configure SCloose @13:27:15.32 SE.configure SEconfig @13:27:15.35 SS.configure SSconfig @13:27:15.38 CH.configure CHconfig @13:27:15.40 PH.configure PHconfig @13:27:15.43 Valve.reset @13:27:16.05 PCR.off @13:27:16.08 PCR.pidtemp= 50,0.1,0,300,25,25 @13:27:16.11 PCR.caltemp= 6.6661,0.0344,0.0 @13:27:16.21 Intake.select :close @13:27:16.24 Exhaust.select :close CTV.dial :air @13:27:16.30 PSV.dial :air @13:27:16.32
PC.reconfigure PCconfig @13:27:16.38 PC.home.seek open @13:27:16.45 CSV.dial :waste1 @13:27:16.87 SSV.dial :spit,avoiding => exhaust @13:27:16.93 PS.reconfigure PSconfig @13:27:16.99 PS.home.seek 0.000ml @13:27:17.74 CS.reconfigure CSfast @13:27:17.77 SS.reconfigure SSconfig @13:27:17.86 SS.home.seek 0.00ml @13:27:17.88 CS.home.seek 0.000ml @13:27:17.96 CC.reconfigure CCconfig @13:27:18.00 CC.home.seek open @13:27:27.21 PS.coast @13:27:27.29 PC.seek open @13:27:27.31
CC.seek open @13:27:27.44 Forearm.reconfigure LooseForearm @13:27:27.47 Forearm.home.seek retract @13:27:28.23 Hand.grip @13:27:28.25
Elbow.jog -1500 @13:27:28.80 Elbow.reconfigure LooseElbow @13:27:28.84 Elbow.home.seek clear @13:27:33.53 SE.reconfigure SEconfig @13:27:33.56 SE.home.seek down @13:27:34.10 SC.reconfigure SCsloppy @13:27:34.13 SC.home.seek initial @13:27:37.88 Forearm.seek retract @13:27:37.94 Elbow.seek hammer @13:27:39.12 Forearm.reconfigure TightForearm @13:27:39.17 Forearm.seek 2 @13:27:40.27 Forearm.coast @13:27:40.30 Arm::Error in MAIN -- Holding position 2 requires -38mA Retry #1 of 5 @13:27:40.31 Hammering Forearm against both stops to recenter bearing races @13:27:40.36 Forearm.force 100 @13:27:40.92 Forearm.coast @13:27:40.94 Forearm.force -100 @13:27:41.51 Forearm.coast @13:27:41.84 Forearm.seek 2 @13:27:42.22 Forearm.seek retract @13:27:43.31 Forearm.reconfigure LooseForearm @13:27:43.74PDT14-Mar-14 ===> STARTED wqPCR (phase #11) <=== @13:27:43.75 Starting initialPurge @13:27:43.76 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @13:27:43.81 Hand.release @13:27:44.61 Elbow.reconfigure TightElbow @13:27:44.64 Elbow.seek FlushPuck::Garage @13:27:46.37 Elbow.reconfigure LooseElbow @13:27:46.46 Forearm.seek garage @13:27:47.49 Hand.grip @13:27:48.36 Forearm.seek retract @13:27:49.38 Elbow.reconfigure TightElbow @13:27:49.42 Elbow.seek CC @13:27:52.17 Elbow.reconfigure LooseElbow @13:27:52.26 Forearm.reconfigure TightForearm @13:27:52.29 Forearm.seek CC @13:27:53.38 CC.seek closed @13:27:57.12 Collection Clamp onSpring/offSpring for 5/22 samples, on/off torque 1.93 @13:27:57.17 Hand.release @13:27:57.93 Forearm.reconfigure LooseForearm @13:27:57.96 Forearm.seek retract @13:27:58.94 Flushing the Collection Valve::Manifold @13:27:59.03 CS Pulling 5ml of flush @13:27:59.04 CSV.dial CSR @13:28:00.31 CSR.select :flush @13:28:00.41 CS.reconfigure CSconfig @13:28:00.44 CS.seek 5.000ml @13:28:13.87 delay 5 seconds @13:28:18.88 CSR.select :air @13:28:19.02 CS.reconfigure CSfast @13:28:19.05 CS.seek 7.500ml @13:28:25.74 CSV.dial :waste @13:28:26.99 CS.reconfigure CSconfig @13:28:27.03 CS.seek 0.000ml @13:28:46.84 Purging external Intake @13:28:46.93 CSV.dial :flush @13:28:47.36 CS.reconfigure CSfast @13:28:47.39 CS.seek 10.000ml @13:29:11.27 delay 5 seconds @13:29:16.29 CSV.dial CBV @13:29:16.31
CBV.dial CSV @13:29:17.10 CTV.dial :intake @13:29:17.80 Intake.select :open @13:29:17.90 CS.seek 0.000ml @13:29:41.99 Intake.select :close @13:29:42.08 CTV.dial :air @13:29:42.82 Flushing upper manifold @13:29:42.91 CSV.dial :flush @13:29:43.70 CS.seek 5.000ml @13:29:56.02 delay 5 seconds @13:30:01.03 CSV.dial :air @13:30:01.49 CS.seek 7.000ml @13:30:06.90 CSV.dial CBV @13:30:06.92
CBV.dial CSV @13:30:07.99 CTV.dial :air @13:30:08.01 CS.seek 0.000ml @13:30:24.74 delay 7 seconds @13:30:31.75 CS.seek 3.000ml @13:30:39.52 CSV.dial :waste @13:30:40.03 CS.seek 0.000ml @13:30:47.82 CSV.dial :flush @13:30:48.25 CS.seek 3.000ml @13:30:56.02 delay 5 seconds @13:31:01.03 CSV.dial :air @13:31:01.51 CS.seek 5.000ml @13:31:06.96 CSV.dial :air @13:31:06.97 CS.seek 0.000ml @13:31:19.17 delay 7 seconds @13:31:26.18 CS.seek 3.000ml @13:31:34.02 CSV.dial :waste @13:31:34.81 CS.seek 0.000ml @13:31:42.49 Garaging FlushPuck in Collection Clamp @13:31:42.55 Elbow.reconfigure TightElbow @13:31:42.58 Elbow.seek CC @13:31:42.67 Elbow.reconfigure LooseElbow @13:31:42.75 Forearm.seek CC @13:31:43.69 CC.seek sealed @13:31:44.61 Hand.grip @13:31:45.38 CC.seek open @13:31:48.67 Forearm.seek retract @13:31:49.60 Elbow.reconfigure TightElbow @13:31:49.64 Elbow.seek garage @13:31:52.42 Elbow.reconfigure LooseElbow @13:31:52.52 Forearm.reconfigure TightForearm @13:31:52.61 Forearm.seek garage @13:31:53.76 Hand.release @13:31:54.54 Forearm.reconfigure LooseForearm @13:31:54.57 Forearm.seek retract @13:31:55.69 Starting mfb @13:31:55.70 pcrBAC sampling at most 1000ml, wcr at most 1000ml @13:31:55.72 Loading sh1 puck from tube 3 into Collection Clamp @13:31:55.75 BACfans.email "Can@13:27:43, 19.5C, 60% humidity, 14.4psia, 13.719V, 0.610A, 0.610A avg, 8.37W\nCTD data unavailable\nISUS data unavailable",Subject => "pcrBAC sampling at most 1000ml, wcr at most 1000ml" @13:31:56.54 Readying the MFB @13:31:56.57 Homing MFB Syringe AFV.select :holdingCoil @13:31:56.71
SC.reconfigure SCtight @13:31:56.74 SC.seek 3 @13:31:56.83 ATV.dial :air2 @13:31:58.12 AS.reconfigure ASconfig @13:31:58.17 AS.home.seek home @13:31:59.02
SC.reconfigure SCloose @13:31:59.76 Elbow.reconfigure TightElbow @13:31:59.79 Elbow.seek 3 @13:32:01.63 Forearm.reconfigure TightForearm @13:32:01.66 Forearm.seek 3 + 800 counts @13:32:02.72 SE.reconfigure SEpick @13:32:02.78 SE.seek beamBreak @13:32:04.41 SE.configure SEconfig @13:32:04.53 Elbow.seek 3 @13:32:04.69 Forearm.seek 3 + 300 counts @13:32:04.90 Forearm.reconfigure LooseForearm @13:32:04.94 Hand.grip @13:32:05.65 SE.seek down @13:32:05.74 AFV.select :carrierSolution @13:32:05.84
Forearm.seek retract @13:32:05.88 delay 2 seconds @13:32:06.83
Elbow.seek CC @13:32:07.11 SE.coast @13:32:07.89 AS.seek 1.000ml @13:32:08.37
Elbow.reconfigure LooseElbow @13:32:08.45 Forearm.reconfigure TightForearm @13:32:08.48 Forearm.seek CC @13:32:09.63 CC.seek closed @13:32:13.41 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.97 @13:32:13.42 Hand.release @13:32:14.17 Forearm.reconfigure LooseForearm @13:32:14.20 Forearm.seek retract @13:32:15.18 SC.coast @13:32:15.21 collectSample 1000ml w/bubble point of 55psi @13:32:15.22 Priming 7ml Sleepy.power :sampler,:ON @13:32:15.23 Intake.select :close Exhaust.select :close @13:32:15.24 SS.hold @13:32:15.41 Calibrating pressure and temperature sensors @13:32:15.42 CTV.dial :air @13:32:15.43 SSV.dial :spit,avoiding => exhaust @13:32:15.45 SS.reconfigure SSfast @13:32:15.48 SS.seek 5.00ml @13:32:18.28 SSV.dial CBV @13:32:18.30
CBV.dial SSV @13:32:19.68 delay 3.8 seconds @13:32:23.56 SSV.dial :spit @13:32:24.90 SS.seek 0.00ml @13:32:27.60 SSV.dial CBV @13:32:28.93 CTV.dial :intake @13:32:29.69 Waiting for external sampler to finish priming... @13:32:29.70 delayUntil "13:42:15.22" @13:32:59.65 delay 1 second @13:33:00.68 AFV.select :holdingCoil @13:33:00.78 delay 1 second @13:33:01.79 ATV.dial :waste @13:33:02.70 AS.reconfigure ASnorm @13:33:02.73 AS.home.seek empty @13:33:56.01 ATV.dial :waste @13:33:56.02 ARV.dial between carrier and air1 @13:33:56.09 ASV.dial :waste1 @13:42:15.23 SSV.dial CBV @13:42:15.25
CBV.dial SSV @13:42:15.26 Allowing 55s for 7.0ml of remaining 7.0ml Intake.select :open @13:42:17.39 Intake @38.0psia, vacuum from 20.0 to 24.0psi @13:42:17.40 SS.reconfigure forSampling @13:42:17.44 SS.seek 7.00ml @13:42:23.11 Stroke completed -- waiting for pressure to equalize ... @13:42:23.74 Intake.select :close @13:42:23.87 SSV.dial :exhaust @13:42:24.66 Exhaust.select :open @13:42:24.75 SS.reconfigure SSexhale @13:42:24.79 SS.seek 0.00ml @13:42:28.55 Exhaust.select :close @13:42:28.65 Sampling 1000.0ml @13:42:28.66 Intake.select :close @13:42:28.67 Exhaust.select :close @13:42:28.68 SS.reconfigure SSconfig @13:42:28.71 SS.hold @13:42:28.85 CTV.dial :intake @13:42:28.86 SSV.dial CBV @13:42:28.88
CBV.dial SSV @13:42:29.70 Allowing 185s for 25.0ml of remaining 1000.0ml Intake.select :open @13:42:31.83 Intake @38.2psia, vacuum from 20.0 to 24.0psi @13:42:31.84 SS.reconfigure forSampling @13:42:31.88 SS.seek 25.00ml @13:42:46.39 Stroke completed -- waiting for pressure to equalize ... @13:42:47.02 Intake.select :close @13:42:47.15 SSV.dial :exhaust @13:42:47.96 Exhaust.select :open @13:42:48.05 SS.reconfigure SSexhale @13:42:48.09 SS.seek 0.00ml @13:42:57.00 Exhaust.select :close @13:42:57.09 SSV.dial CBV @13:42:57.11
CBV.dial SSV @13:42:57.91 Allowing 185s for 25.0ml of remaining 975.0ml @13:42:57.92 Intake.select :open @13:43:00.05 Intake @37.9psia, vacuum from 20.0 to 24.0psi @13:43:00.06 SS.reconfigure forSampling @13:43:00.10 SS.seek 25.00ml @13:43:14.60 Stroke completed -- waiting for pressure to equalize ... @13:43:15.23 Intake.select :close @13:43:15.37 SSV.dial :exhaust @13:43:16.17 Exhaust.select :open @13:43:16.26 SS.reconfigure SSexhale @13:43:16.30 SS.seek 0.00ml @13:43:25.27 Exhaust.select :close @13:43:25.36 SSV.dial CBV @13:43:25.38
CBV.dial SSV @13:43:26.17 Allowing 185s for 25.0ml of remaining 950.0ml @13:43:26.18 Intake.select :open @13:43:28.31 Intake @37.6psia, vacuum from 20.0 to 24.0psi @13:43:28.32 SS.reconfigure forSampling @13:43:28.36 SS.seek 25.00ml @13:43:42.81 Stroke completed -- waiting for pressure to equalize ... @13:43:43.44 Intake.select :close @13:43:43.57 SSV.dial :exhaust @13:43:44.92 Exhaust.select :open @13:43:45.01 SS.reconfigure SSexhale @13:43:45.05 SS.seek 0.00ml @13:43:54.00 Exhaust.select :close @13:43:54.09 SSV.dial CBV @13:43:54.11
CBV.dial SSV @13:43:54.92 Allowing 185s for 25.0ml of remaining 925.0ml @13:43:54.93 Intake.select :open @13:43:57.06 Intake @38.1psia, vacuum from 20.0 to 24.0psi @13:43:57.07 SS.reconfigure forSampling @13:43:57.11 SS.seek 25.00ml @13:44:11.81 Stroke completed -- waiting for pressure to equalize ... @13:44:12.45 Intake.select :close @13:44:12.58 SSV.dial :exhaust @13:44:13.39 Exhaust.select :open @13:44:13.48 SS.reconfigure SSexhale @13:44:13.52 SS.seek 0.00ml @13:44:22.40 Exhaust.select :close @13:44:22.49 SSV.dial CBV @13:44:22.51
CBV.dial SSV @13:44:23.33 Allowing 185s for 25.0ml of remaining 900.0ml @13:44:23.34 Intake.select :open @13:44:25.47 Intake @38.4psia, vacuum from 20.0 to 24.0psi @13:44:25.48 SS.reconfigure forSampling @13:44:25.52 SS.seek 25.00ml @13:44:36.21 Vacuum too high -- waiting up to 40s @13:44:36.48 Intake @30.5psia, vacuum from 20.0 to 24.0psi @13:44:36.49 SS.reconfigure forSampling @13:44:36.52 SS.seek 25.00ml @13:44:40.84 Stroke completed -- waiting for pressure to equalize ... @13:44:41.47 Intake.select :close @13:44:41.61 SSV.dial :exhaust @13:44:42.40 Exhaust.select :open @13:44:42.49 SS.reconfigure SSexhale @13:44:42.53 SS.seek 0.00ml @13:44:51.45 Exhaust.select :close @13:44:51.54 SSV.dial CBV @13:44:51.56
CBV.dial SSV @13:44:52.36 Allowing 185s for 25.0ml of remaining 875.0ml @13:44:52.37 Intake.select :open @13:44:54.50 Intake @38.6psia, vacuum from 20.0 to 24.0psi @13:44:54.51 SS.reconfigure forSampling @13:44:54.55 SS.seek 25.00ml @13:45:10.34 Stroke completed -- waiting for pressure to equalize ... @13:45:10.97 Intake.select :close @13:45:11.11 SSV.dial :exhaust @13:45:11.90 Exhaust.select :open @13:45:11.99 SS.reconfigure SSexhale @13:45:12.03 SS.seek 0.00ml @13:45:21.51 Exhaust.select :close @13:45:21.60 SSV.dial CBV @13:45:21.62
CBV.dial SSV @13:45:22.42 Allowing 185s for 25.0ml of remaining 850.0ml @13:45:22.43 Intake.select :open @13:45:24.56 Intake @38.5psia, vacuum from 20.0 to 24.0psi @13:45:24.57 SS.reconfigure forSampling @13:45:24.61 SS.seek 25.00ml @13:45:32.30 Vacuum too high -- waiting up to 40s @13:45:32.57 Intake @31.6psia, vacuum from 20.0 to 24.0psi @13:45:32.58 SS.reconfigure forSampling @13:45:32.61 SS.seek 25.00ml @13:45:41.56 Stroke completed -- waiting for pressure to equalize ... @13:45:42.19 Intake.select :close @13:45:42.32 SSV.dial :exhaust @13:45:43.12 Exhaust.select :open @13:45:43.21 SS.reconfigure SSexhale @13:45:43.25 SS.seek 0.00ml @13:45:52.27 Exhaust.select :close @13:45:52.36 SSV.dial CBV @13:45:52.38
CBV.dial SSV @13:45:53.18 Allowing 185s for 25.0ml of remaining 825.0ml @13:45:53.19 Intake.select :open @13:45:55.41 Intake @38.5psia, vacuum from 20.0 to 24.0psi @13:45:55.42 SS.reconfigure forSampling @13:45:55.45 SS.seek 25.00ml @13:46:04.15 Vacuum too high -- waiting up to 40s @13:46:04.40 Intake @32.6psia, vacuum from 20.0 to 24.0psi @13:46:04.41 SS.reconfigure forSampling @13:46:04.45 SS.seek 25.00ml @13:46:14.29 Stroke completed -- waiting for pressure to equalize ... @13:46:14.92 Intake.select :close @13:46:15.05 SSV.dial :exhaust @13:46:15.85 Exhaust.select :open @13:46:15.94 SS.reconfigure SSexhale @13:46:15.98 SS.seek 0.00ml @13:46:24.90 Exhaust.select :close @13:46:24.99 SSV.dial CBV @13:46:25.01
CBV.dial SSV @13:46:25.80 Allowing 185s for 25.0ml of remaining 800.0ml @13:46:25.81 Intake.select :open @13:46:27.95 Intake @38.8psia, vacuum from 20.0 to 24.0psi SS.reconfigure forSampling @13:46:27.99 SS.seek 25.00ml @13:46:36.21 Vacuum too high -- waiting up to 40s @13:46:36.47 Intake @33.2psia, vacuum from 20.0 to 24.0psi @13:46:36.48 SS.reconfigure forSampling @13:46:36.52 SS.seek 25.00ml @13:46:49.41 Stroke completed -- waiting for pressure to equalize ... @13:46:50.04 Intake.select :close @13:46:50.18 SSV.dial :exhaust @13:46:50.98 Exhaust.select :open @13:46:51.07 SS.reconfigure SSexhale @13:46:51.11 SS.seek 0.00ml @13:47:00.08 Exhaust.select :close @13:47:00.17 SSV.dial CBV @13:47:00.19
CBV.dial SSV @13:47:00.99 Allowing 185s for 25.0ml of remaining 775.0ml @13:47:01.00 Intake.select :open @13:47:03.13 Intake @38.3psia, vacuum from 20.0 to 24.0psi @13:47:03.14 SS.reconfigure forSampling @13:47:03.18 SS.seek 25.00ml @13:47:11.58 Vacuum too high -- waiting up to 40s @13:47:11.84 Intake @33.9psia, vacuum from 20.0 to 24.0psi @13:47:11.85 SS.reconfigure forSampling @13:47:11.89 SS.seek 25.00ml @13:47:28.14 Stroke completed -- waiting for pressure to equalize ... @13:47:28.77 Intake.select :close @13:47:28.90 SSV.dial :exhaust @13:47:29.69 Exhaust.select :open @13:47:29.78 SS.reconfigure SSexhale @13:47:29.82 SS.seek 0.00ml @13:47:38.82 Exhaust.select :close @13:47:38.91 SSV.dial CBV @13:47:38.93
CBV.dial SSV @13:47:39.72 Allowing 185s for 25.0ml of remaining 750.0ml @13:47:39.73 Intake.select :open @13:47:41.86 Intake @37.7psia, vacuum from 20.0 to 24.0psi @13:47:41.87 SS.reconfigure forSampling @13:47:41.90 SS.seek 25.00ml @13:47:49.39 Vacuum too high -- waiting up to 40s @13:47:49.65 Intake @34.3psia, vacuum from 20.0 to 24.0psi @13:47:49.66 SS.reconfigure forSampling @13:47:49.70 SS.seek 25.00ml @13:48:12.55 Stroke completed -- waiting for pressure to equalize ... @13:48:13.18 Intake.select :close @13:48:13.31 SSV.dial :exhaust @13:48:14.11 Exhaust.select :open @13:48:14.20 SS.reconfigure SSexhale @13:48:14.24 SS.seek 0.00ml @13:48:23.59 Exhaust.select :close @13:48:23.68 SSV.dial CBV @13:48:23.70
CBV.dial SSV @13:48:24.50 Allowing 185s for 25.0ml of remaining 725.0ml @13:48:24.51 Intake.select :open @13:48:26.64 Intake @37.6psia, vacuum from 20.0 to 24.0psi @13:48:26.65 SS.reconfigure forSampling @13:48:26.69 SS.seek 25.00ml @13:48:40.11 Vacuum too high -- waiting up to 40s @13:48:40.37 Intake @35.6psia, vacuum from 20.0 to 24.0psi @13:48:40.38 SS.reconfigure forSampling @13:48:40.42 SS.seek 25.00ml @13:49:03.16 Stroke completed -- waiting for pressure to equalize ... @13:49:03.79 Intake.select :close @13:49:03.92 SSV.dial :exhaust @13:49:04.73 Exhaust.select :open @13:49:04.82 SS.reconfigure SSexhale @13:49:04.86 SS.seek 0.00ml @13:49:13.81 Exhaust.select :close @13:49:14.55 CBV.dial SSV @13:49:14.56 SSV.dial CBV @13:49:15.37
Allowing 185s for 25.0ml of remaining 700.0ml @13:49:15.38 Intake.select :open @13:49:17.53 Intake @37.6psia, vacuum from 20.0 to 24.0psi @13:49:17.54 SS.reconfigure forSampling @13:49:17.58 SS.seek 25.00ml @13:49:46.82 Vacuum too high -- waiting up to 40s @13:49:47.09 Intake @36.3psia, vacuum from 20.0 to 24.0psi @13:49:47.10 SS.reconfigure forSampling @13:49:47.13 SS.seek 25.00ml @13:50:03.51 Stroke completed -- waiting for pressure to equalize ... @13:50:04.15 Intake.select :close @13:50:04.28 SSV.dial :exhaust @13:50:05.08 Exhaust.select :open @13:50:05.17 SS.reconfigure SSexhale @13:50:05.21 SS.seek 0.00ml @13:50:14.20 Exhaust.select :close @13:50:14.29 SSV.dial CBV @13:50:14.31
CBV.dial SSV @13:50:15.10 Allowing 185s for 25.0ml of remaining 675.0ml @13:50:15.11 Intake.select :open @13:50:17.24 Intake @39.4psia, vacuum from 20.0 to 24.0psi @13:50:17.25 SS.reconfigure forSampling @13:50:17.29 SS.seek 25.00ml @13:51:16.80 Stroke completed -- waiting for pressure to equalize ... @13:51:18.02 Intake.select :close @13:51:18.15 SSV.dial :exhaust @13:51:18.95 Exhaust.select :open @13:51:19.04 SS.reconfigure SSexhale @13:51:19.08 SS.seek 0.00ml @13:51:28.12 Exhaust.select :close @13:51:28.21 SSV.dial CBV @13:51:28.23
CBV.dial SSV @13:51:29.02 Allowing 185s for 25.0ml of remaining 650.0ml @13:51:29.03 Intake.select :open @13:51:31.16 Intake @38.8psia, vacuum from 20.0 to 24.0psi @13:51:31.17 SS.reconfigure forSampling @13:51:31.21 SS.seek 25.00ml @13:51:56.10 Vacuum too high -- waiting up to 40s @13:51:56.36 Intake @38.5psia, vacuum from 20.0 to 24.0psi @13:51:56.37 SS.reconfigure forSampling @13:51:56.40 SS.seek 25.00ml @13:53:01.17 Stroke completed -- waiting for pressure to equalize ... @13:53:02.38 Intake.select :close @13:53:02.52 SSV.dial :exhaust @13:53:03.31 Exhaust.select :open @13:53:03.40 SS.reconfigure SSexhale @13:53:03.44 SS.seek 0.00ml @13:53:12.39 Exhaust.select :close @13:53:12.48 SSV.dial CBV @13:53:12.50
CBV.dial SSV @13:53:13.30 Allowing 185s for 25.0ml of remaining 625.0ml @13:53:13.31 Intake.select :open @13:53:15.44 Intake @39.4psia, vacuum from 20.0 to 24.0psi @13:53:15.45 SS.reconfigure forSampling @13:53:15.49 SS.seek 25.00ml @13:55:05.96 Stroke completed -- waiting for pressure to equalize ... @13:55:07.17 Intake.select :close @13:55:07.30 SSV.dial :exhaust @13:55:08.10 Exhaust.select :open @13:55:08.19 SS.reconfigure SSexhale @13:55:08.23 SS.seek 0.00ml @13:55:17.09 Exhaust.select :close @13:55:17.18 SSV.dial CBV @13:55:17.20
CBV.dial SSV @13:55:17.99 Allowing 185s for 25.0ml of remaining 600.0ml @13:55:18.00 Intake.select :open @13:55:20.13 Intake @46.9psia, vacuum from 20.0 to 24.0psi @13:55:20.14 SS.reconfigure forSampling @13:55:20.18 SS.seek 25.00ml @13:57:10.64 Stroke completed -- waiting for pressure to equalize ... @13:57:12.43 Intake.select :close @13:57:12.57 SSV.dial :exhaust @13:57:13.36 Exhaust.select :open @13:57:13.45 SS.reconfigure SSexhale @13:57:13.49 SS.seek 0.00ml @13:57:22.84 Exhaust.select :close @13:57:22.93 SSV.dial CBV @13:57:22.95
CBV.dial SSV @13:57:23.75 Allowing 185s for 25.0ml of remaining 575.0ml @13:57:23.76 Intake.select :open @13:57:25.89 Intake @47.4psia, vacuum from 20.0 to 24.0psi @13:57:25.90 SS.reconfigure forSampling @13:57:25.94 SS.seek 25.00ml @13:58:26.27 Vacuum too high -- waiting up to 40s @13:58:26.53 Intake @47.1psia, vacuum from 20.0 to 24.0psi @13:58:26.54 SS.reconfigure forSampling @13:58:26.58 SS.seek 25.00ml @13:58:44.62 Vacuum too high -- waiting up to 40s @13:58:44.87 Intake @47.0psia, vacuum from 20.0 to 24.0psi @13:58:44.88 SS.reconfigure forSampling @13:58:44.92 SS.seek 25.00ml @13:58:55.71 Vacuum too high -- waiting up to 40s @13:58:55.97 Intake @47.2psia, vacuum from 20.0 to 24.0psi @13:58:55.98 SS.reconfigure forSampling @13:58:56.02 SS.seek 25.00ml @13:58:59.21 Vacuum too high -- waiting up to 40s @13:58:59.48 SS.seek 25.00ml @13:59:07.27 Vacuum too high -- waiting up to 40s @13:59:07.53 Intake @47.3psia, vacuum from 20.0 to 24.0psi @13:59:07.54 SS.reconfigure forSampling @13:59:07.58 SS.seek 25.00ml @13:59:10.86 Vacuum too high -- waiting up to 40s @13:59:11.12 Intake @47.2psia, vacuum from 20.0 to 24.0psi @13:59:11.13 SS.reconfigure forSampling @13:59:11.17 SS.seek 25.00ml @13:59:13.34 Vacuum too high -- waiting up to 40s @13:59:13.59 Intake @47.1psia, vacuum from 20.0 to 24.0psi @13:59:13.60 SS.reconfigure forSampling @13:59:13.64 SS.seek 25.00ml @13:59:15.69 Vacuum too high -- waiting up to 40s @13:59:15.95 Intake @47.3psia, vacuum from 20.0 to 24.0psi @13:59:15.96 SS.reconfigure forSampling @13:59:15.99 SS.seek 25.00ml @13:59:19.69 Stroke completed -- waiting for pressure to equalize ... @13:59:21.52 Intake.select :close @13:59:21.65 SSV.dial :exhaust @13:59:22.43 Exhaust.select :open @13:59:22.53 SS.reconfigure SSexhale @13:59:22.57 SS.seek 0.00ml @13:59:31.46 Exhaust.select :close @13:59:31.55 SSV.dial CBV @13:59:31.57
CBV.dial SSV @13:59:32.37 Allowing 185s for 25.0ml of remaining 550.0ml @13:59:32.38 Intake.select :open @13:59:34.51 Intake @47.6psia, vacuum from 20.0 to 24.0psi @13:59:34.52 SS.reconfigure forSampling @13:59:34.56 SS.seek 25.00ml @13:59:37.59 Vacuum too high -- waiting up to 40s @13:59:37.85 Intake @47.5psia, vacuum from 20.0 to 24.0psi @13:59:37.86 SS.reconfigure forSampling @13:59:37.89 SS.seek 25.00ml @13:59:52.52 Vacuum too high -- waiting up to 40s @13:59:52.79 Intake @47.6psia, vacuum from 20.0 to 24.0psi @13:59:52.80 SS.reconfigure forSampling @13:59:52.83 SS.seek 25.00ml @13:59:54.43 Vacuum too high -- waiting up to 40s @13:59:54.69 Intake @47.6psia, vacuum from 20.0 to 24.0psi @13:59:54.70 SS.reconfigure forSampling @13:59:54.73 SS.seek 25.00ml @14:00:01.02 Vacuum too high -- waiting up to 40s @14:00:01.29 Intake @47.8psia, vacuum from 20.0 to 24.0psi @14:00:01.30 SS.reconfigure forSampling @14:00:01.33 SS.seek 25.00ml @14:00:02.71 Vacuum too high -- waiting up to 40s @14:00:02.97 Intake @47.5psia, vacuum from 20.0 to 24.0psi @14:00:02.98 SS.reconfigure forSampling @14:00:03.01 SS.seek 25.00ml @14:00:04.53 Vacuum too high -- waiting up to 40s @14:00:04.79 Intake @47.5psia, vacuum from 20.0 to 24.0psi @14:00:04.80 SS.reconfigure forSampling @14:00:04.83 SS.seek 25.00ml @14:00:06.24 Vacuum too high -- waiting up to 40s @14:00:06.50 Intake @47.5psia, vacuum from 20.0 to 24.0psi @14:00:06.51 SS.reconfigure forSampling @14:00:06.54 SS.seek 25.00ml @14:00:07.74 Vacuum too high -- waiting up to 40s @14:00:08.00 Intake @47.5psia, vacuum from 20.0 to 24.0psi @14:00:08.01 SS.reconfigure forSampling @14:00:08.04 SS.seek 25.00ml @14:00:13.24 Vacuum too high -- waiting up to 40s @14:00:13.50 Intake @47.6psia, vacuum from 20.0 to 24.0psi @14:00:13.51 SS.reconfigure forSampling @14:00:13.55 SS.seek 25.00ml @14:00:14.93 Vacuum too high -- waiting up to 40s @14:00:15.19 Intake @47.6psia, vacuum from 20.0 to 24.0psi @14:00:15.20 SS.reconfigure forSampling @14:00:15.24 SS.seek 25.00ml @14:00:16.74 Vacuum too high -- waiting up to 40s @14:00:17.01 Intake @47.6psia, vacuum from 20.0 to 24.0psi @14:00:17.02 SS.reconfigure forSampling @14:00:17.05 SS.seek 25.00ml @14:00:18.28 Vacuum too high -- waiting up to 40s @14:00:18.54 Intake @47.5psia, vacuum from 20.0 to 24.0psi @14:00:18.55 SS.reconfigure forSampling @14:00:18.58 SS.seek 25.00ml @14:00:19.71 Vacuum too high -- waiting up to 40s @14:00:19.96 Intake @47.3psia, vacuum from 20.0 to 24.0psi @14:00:19.97 SS.reconfigure forSampling @14:00:20.01 SS.seek 25.00ml @14:00:21.37 Vacuum too high -- waiting up to 40s @14:00:21.63 Intake @47.4psia, vacuum from 20.0 to 24.0psi @14:00:21.64 SS.reconfigure forSampling @14:00:21.67 SS.seek 25.00ml @14:00:25.70 Vacuum too high -- waiting up to 40s @14:00:25.96 Intake @47.6psia, vacuum from 20.0 to 24.0psi @14:00:25.97 SS.reconfigure forSampling @14:00:26.01 SS.seek 25.00ml @14:00:27.37 Vacuum too high -- waiting up to 40s @14:00:27.62 Intake @47.4psia, vacuum from 20.0 to 24.0psi @14:00:27.63 SS.reconfigure forSampling @14:00:27.67 SS.seek 25.00ml @14:00:28.96 Vacuum too high -- waiting up to 40s @14:00:29.21 Intake @47.4psia, vacuum from 20.0 to 24.0psi @14:00:29.22 SS.reconfigure forSampling @14:00:29.26 SS.seek 25.00ml @14:00:30.59 Vacuum too high -- waiting up to 40s @14:00:30.85 Intake @47.3psia, vacuum from 20.0 to 24.0psi @14:00:30.86 SS.reconfigure forSampling @14:00:30.89 SS.seek 25.00ml @14:00:32.15 Vacuum too high -- waiting up to 40s @14:00:32.40 Intake @47.0psia, vacuum from 20.0 to 24.0psi @14:00:32.41 SS.reconfigure forSampling @14:00:32.45 SS.seek 25.00ml @14:00:33.74 Vacuum too high -- waiting up to 40s @14:00:34.00 Intake @47.3psia, vacuum from 20.0 to 24.0psi @14:00:34.01 SS.reconfigure forSampling @14:00:34.04 SS.seek 25.00ml @14:00:35.90 Vacuum too high -- waiting up to 40s @14:00:36.64 Intake @48.6psia, vacuum from 20.0 to 24.0psi @14:00:36.65 SS.reconfigure forSampling @14:00:36.68 SS.seek 25.00ml @14:00:38.06 Vacuum too high -- waiting up to 40s @14:00:38.32 Intake @47.7psia, vacuum from 20.0 to 24.0psi @14:00:38.33 SS.reconfigure forSampling @14:00:38.37 SS.seek 25.00ml @14:00:39.62 Vacuum too high -- waiting up to 40s @14:00:39.89 SS.seek 25.00ml @14:00:41.02 Vacuum too high -- waiting up to 40s @14:00:41.28 Intake @47.8psia, vacuum from 20.0 to 24.0psi @14:00:41.29 SS.reconfigure forSampling @14:00:41.32 SS.seek 25.00ml @14:00:42.40 Vacuum too high -- waiting up to 40s @14:00:42.66 Intake @47.4psia, vacuum from 20.0 to 24.0psi @14:00:42.67 SS.reconfigure forSampling @14:00:42.70 SS.seek 25.00ml @14:00:43.81 Vacuum too high -- waiting up to 40s @14:00:44.08 SS.seek 25.00ml @14:00:45.24 Vacuum too high -- waiting up to 40s @14:00:45.50 Intake @47.5psia, vacuum from 20.0 to 24.0psi @14:00:45.51 SS.reconfigure forSampling @14:00:45.54 SS.seek 25.00ml @14:00:46.71 Vacuum too high -- waiting up to 40s @14:00:46.97 Intake @47.9psia, vacuum from 20.0 to 24.0psi @14:00:46.98 SS.reconfigure forSampling @14:00:47.01 SS.seek 25.00ml @14:00:48.27 Vacuum too high -- waiting up to 40s @14:00:48.53 Intake @48.1psia, vacuum from 20.0 to 24.0psi @14:00:48.54 SS.reconfigure forSampling @14:00:48.57 SS.seek 25.00ml @14:00:49.55 Vacuum too high -- waiting up to 40s @14:00:49.81 Intake @47.5psia, vacuum from 20.0 to 24.0psi @14:00:49.82 SS.reconfigure forSampling @14:00:49.85 SS.seek 25.00ml @14:00:51.02 Vacuum too high -- waiting up to 40s @14:00:51.28 Intake @47.6psia, vacuum from 20.0 to 24.0psi @14:00:51.29 SS.reconfigure forSampling @14:00:51.32 SS.seek 25.00ml @14:00:52.40 Vacuum too high -- waiting up to 40s @14:00:52.66 Intake @47.5psia, vacuum from 20.0 to 24.0psi @14:00:52.67 SS.reconfigure forSampling @14:00:52.70 SS.seek 25.00ml @14:00:53.77 Vacuum too high -- waiting up to 40s @14:00:54.03 Intake @47.1psia, vacuum from 20.0 to 24.0psi @14:00:54.04 SS.reconfigure forSampling @14:00:54.07 SS.seek 25.00ml @14:00:55.30 Vacuum too high -- waiting up to 40s @14:00:55.56 Intake @47.4psia, vacuum from 20.0 to 24.0psi @14:00:55.57 SS.reconfigure forSampling @14:00:55.60 SS.seek 25.00ml @14:00:56.71 Vacuum too high -- waiting up to 40s @14:00:56.97 Intake @47.3psia, vacuum from 20.0 to 24.0psi @14:00:56.98 SS.reconfigure forSampling @14:00:57.01 SS.seek 25.00ml @14:00:58.27 Vacuum too high -- waiting up to 40s @14:00:58.53 Intake @47.6psia, vacuum from 20.0 to 24.0psi @14:00:58.54 SS.reconfigure forSampling @14:00:58.57 SS.seek 25.00ml @14:00:59.65 Vacuum too high -- waiting up to 40s @14:00:59.91 Intake @47.9psia, vacuum from 20.0 to 24.0psi @14:00:59.92 SS.reconfigure forSampling @14:00:59.95 SS.seek 25.00ml @14:01:00.96 Vacuum too high -- waiting up to 40s @14:01:01.23 SS.seek 25.00ml @14:01:02.24 Vacuum too high -- waiting up to 40s @14:01:02.50 Intake @47.7psia, vacuum from 20.0 to 24.0psi @14:01:02.51 SS.reconfigure forSampling @14:01:02.54 SS.seek 25.00ml @14:01:03.71 Vacuum too high -- waiting up to 40s @14:01:03.97 Intake @47.6psia, vacuum from 20.0 to 24.0psi @14:01:03.98 SS.reconfigure forSampling @14:01:04.02 SS.seek 25.00ml @14:01:05.12 Vacuum too high -- waiting up to 40s @14:01:05.38 Intake @47.7psia, vacuum from 20.0 to 24.0psi @14:01:05.39 SS.reconfigure forSampling @14:01:05.42 SS.seek 25.00ml @14:01:06.46 Vacuum too high -- waiting up to 40s @14:01:06.72 Intake @47.4psia, vacuum from 20.0 to 24.0psi @14:01:06.73 SS.reconfigure forSampling @14:01:06.76 SS.seek 25.00ml @14:01:07.90 Vacuum too high -- waiting up to 40s @14:01:08.17 SS.seek 25.00ml @14:01:09.21 Vacuum too high -- waiting up to 40s @14:01:09.47 Intake @47.2psia, vacuum from 20.0 to 24.0psi @14:01:09.48 SS.reconfigure forSampling @14:01:09.51 SS.seek 25.00ml @14:01:10.62 Vacuum too high -- waiting up to 40s @14:01:10.88 Intake @47.1psia, vacuum from 20.0 to 24.0psi @14:01:10.89 SS.reconfigure forSampling @14:01:10.92 SS.seek 25.00ml @14:01:12.12 Vacuum too high -- waiting up to 40s @14:01:12.91 Intake @47.9psia, vacuum from 20.0 to 24.0psi @14:01:12.92 SS.reconfigure forSampling @14:01:12.95 SS.seek 25.00ml @14:01:14.40 Vacuum too high -- waiting up to 40s @14:01:14.66 Intake @47.7psia, vacuum from 20.0 to 24.0psi @14:01:14.67 SS.reconfigure forSampling @14:01:14.70 SS.seek 25.00ml @14:01:15.71 Vacuum too high -- waiting up to 40s @14:01:15.97 Intake @47.5psia, vacuum from 20.0 to 24.0psi @14:01:15.98 SS.reconfigure forSampling @14:01:16.02 SS.seek 25.00ml @14:01:17.21 Vacuum too high -- waiting up to 40s @14:01:17.48 Intake @47.4psia, vacuum from 20.0 to 24.0psi @14:01:17.49 SS.reconfigure forSampling @14:01:17.52 SS.seek 25.00ml @14:01:18.58 Vacuum too high -- waiting up to 40s @14:01:18.84 Intake @47.3psia, vacuum from 20.0 to 24.0psi @14:01:18.85 SS.reconfigure forSampling @14:01:18.89 SS.seek 25.00ml @14:01:19.99 Vacuum too high -- waiting up to 40s @14:01:20.30 Intake @47.2psia, vacuum from 20.0 to 24.0psi @14:01:20.31 SS.reconfigure forSampling @14:01:20.40 SS.seek 25.00ml @14:01:21.68 Vacuum too high -- waiting up to 40s @14:01:21.94 Intake @47.5psia, vacuum from 20.0 to 24.0psi @14:01:21.95 SS.reconfigure forSampling @14:01:21.98 SS.seek 25.00ml @14:01:23.12 Vacuum too high -- waiting up to 40s @14:01:23.38 Intake @47.6psia, vacuum from 20.0 to 24.0psi @14:01:23.39 SS.reconfigure forSampling @14:01:23.42 SS.seek 25.00ml @14:01:24.46 Vacuum too high -- waiting up to 40s @14:01:24.72 Intake @48.1psia, vacuum from 20.0 to 24.0psi @14:01:24.73 SS.reconfigure forSampling @14:01:24.77 SS.seek 25.00ml @14:01:25.77 Vacuum too high -- waiting up to 40s @14:01:26.03 Intake @47.6psia, vacuum from 20.0 to 24.0psi @14:01:26.04 SS.reconfigure forSampling @14:01:26.07 SS.seek 25.00ml @14:01:27.08 Vacuum too high -- waiting up to 40s @14:01:27.34 Intake @47.8psia, vacuum from 20.0 to 24.0psi @14:01:27.35 SS.reconfigure forSampling @14:01:27.38 SS.seek 25.00ml @14:01:28.40 Vacuum too high -- waiting up to 40s @14:01:28.66 Intake @47.6psia, vacuum from 20.0 to 24.0psi @14:01:28.67 SS.reconfigure forSampling @14:01:28.70 SS.seek 25.00ml @14:01:29.77 Vacuum too high -- waiting up to 40s @14:01:30.03 Intake @47.5psia, vacuum from 20.0 to 24.0psi @14:01:30.04 SS.reconfigure forSampling @14:01:30.07 SS.seek 25.00ml @14:01:31.05 Vacuum too high -- waiting up to 40s @14:01:31.31 Intake @47.4psia, vacuum from 20.0 to 24.0psi @14:01:31.32 SS.reconfigure forSampling @14:01:31.35 SS.seek 25.00ml @14:01:32.49 Vacuum too high -- waiting up to 40s @14:01:32.75 Intake @47.3psia, vacuum from 20.0 to 24.0psi @14:01:32.76 SS.reconfigure forSampling @14:01:32.79 SS.seek 25.00ml @14:01:33.93 Vacuum too high -- waiting up to 40s @14:01:34.19 Intake @47.4psia, vacuum from 20.0 to 24.0psi @14:01:34.20 SS.reconfigure forSampling @14:01:34.23 SS.seek 25.00ml @14:01:35.27 Vacuum too high -- waiting up to 40s @14:01:35.55 SS.seek 25.00ml @14:01:36.58 Vacuum too high -- waiting up to 40s @14:01:36.84 Intake @48.2psia, vacuum from 20.0 to 24.0psi @14:01:36.85 SS.reconfigure forSampling @14:01:36.88 SS.seek 25.00ml @14:01:37.93 Vacuum too high -- waiting up to 40s @14:01:38.19 Intake @48.4psia, vacuum from 20.0 to 24.0psi @14:01:38.20 SS.reconfigure forSampling @14:01:38.23 SS.seek 25.00ml @14:01:39.11 Vacuum too high -- waiting up to 40s @14:01:39.37 Intake @47.5psia, vacuum from 20.0 to 24.0psi @14:01:39.38 SS.reconfigure forSampling @14:01:39.41 SS.seek 25.00ml @14:01:40.49 Vacuum too high -- waiting up to 40s @14:01:40.75 Intake @47.5psia, vacuum from 20.0 to 24.0psi @14:01:40.76 SS.reconfigure forSampling @14:01:40.79 SS.seek 25.00ml @14:01:41.80 Vacuum too high -- waiting up to 40s @14:01:42.06 Intake @47.8psia, vacuum from 20.0 to 24.0psi @14:01:42.07 SS.reconfigure forSampling @14:01:42.10 SS.seek 25.00ml @14:01:42.99 Vacuum too high -- waiting up to 40s @14:01:43.25 Intake @47.7psia, vacuum from 20.0 to 24.0psi @14:01:43.26 SS.reconfigure forSampling @14:01:43.29 SS.seek 25.00ml @14:01:44.36 Vacuum too high -- waiting up to 40s @14:01:44.62 Intake @47.5psia, vacuum from 20.0 to 24.0psi @14:01:44.63 SS.reconfigure forSampling @14:01:44.66 SS.seek 25.00ml @14:01:45.68 Vacuum too high -- waiting up to 40s @14:01:45.94 Intake @47.6psia, vacuum from 20.0 to 24.0psi @14:01:45.95 SS.reconfigure forSampling @14:01:45.98 SS.seek 25.00ml @14:01:47.83 Vacuum too high -- waiting up to 40s @14:01:48.09 Intake @47.3psia, vacuum from 20.0 to 24.0psi @14:01:48.10 SS.reconfigure forSampling @14:01:48.13 SS.seek 25.00ml @14:01:49.27 Vacuum too high -- waiting up to 40s @14:01:49.53 Intake @47.4psia, vacuum from 20.0 to 24.0psi @14:01:49.54 SS.reconfigure forSampling @14:01:49.57 SS.seek 25.00ml @14:01:51.00 Vacuum too high -- waiting up to 40s @14:01:51.26 Intake @48.3psia, vacuum from 20.0 to 24.0psi @14:01:51.27 SS.reconfigure forSampling @14:01:51.30 SS.seek 25.00ml @14:01:52.46 Vacuum too high -- waiting up to 40s @14:01:52.73 Intake @48.1psia, vacuum from 20.0 to 24.0psi @14:01:52.74 SS.reconfigure forSampling @14:01:52.77 SS.seek 25.00ml @14:01:53.74 Vacuum too high -- waiting up to 40s @14:01:54.00 Intake @47.7psia, vacuum from 20.0 to 24.0psi @14:01:54.01 SS.reconfigure forSampling @14:01:54.05 SS.seek 25.00ml @14:01:55.08 Vacuum too high -- waiting up to 40s @14:01:55.34 Intake @47.8psia, vacuum from 20.0 to 24.0psi @14:01:55.35 SS.reconfigure forSampling @14:01:55.39 SS.seek 25.00ml @14:01:56.36 Vacuum too high -- waiting up to 40s @14:01:56.62 SS.seek 25.00ml @14:01:57.49 Vacuum too high -- waiting up to 40s @14:01:57.75 Intake @47.5psia, vacuum from 20.0 to 24.0psi @14:01:57.76 SS.reconfigure forSampling @14:01:57.80 SS.seek 25.00ml @14:01:58.77 Vacuum too high -- waiting up to 40s @14:01:59.03 Intake @47.4psia, vacuum from 20.0 to 24.0psi @14:01:59.04 SS.reconfigure forSampling @14:01:59.08 SS.seek 25.00ml @14:02:00.02 Vacuum too high -- waiting up to 40s @14:02:00.28 Intake @47.5psia, vacuum from 20.0 to 24.0psi @14:02:00.29 SS.reconfigure forSampling @14:02:00.33 SS.seek 25.00ml @14:02:01.21 Vacuum too high -- waiting up to 40s @14:02:01.47 Intake @47.3psia, vacuum from 20.0 to 24.0psi @14:02:01.48 SS.reconfigure forSampling @14:02:01.51 SS.seek 25.00ml @14:02:02.58 Vacuum too high -- waiting up to 40s @14:02:03.05 Intake @47.2psia, vacuum from 20.0 to 24.0psi @14:02:03.06 SS.reconfigure forSampling @14:02:03.09 SS.seek 25.00ml @14:02:03.94 Stroke completed -- waiting for pressure to equalize ... @14:02:05.77 Intake.select :close @14:02:05.90 SSV.dial :exhaust @14:02:06.69 Exhaust.select :open @14:02:06.78 SS.reconfigure SSexhale @14:02:06.82 SS.seek 0.00ml @14:02:15.81 Exhaust.select :close @14:02:15.90 SSV.dial CBV @14:02:15.92
CBV.dial SSV @14:02:16.73 Allowing 185s for 25.0ml of remaining 525.0ml @14:02:16.74 Intake.select :open @14:02:18.87 Intake @47.5psia, vacuum from 20.0 to 24.0psi @14:02:18.88 SS.reconfigure forSampling @14:02:18.92 SS.seek 25.00ml @14:02:20.64 Vacuum too high -- waiting up to 40s @14:02:20.89 Intake @47.4psia, vacuum from 20.0 to 24.0psi @14:02:20.90 SS.reconfigure forSampling @14:02:20.94 SS.seek 25.00ml @14:02:21.83 Vacuum too high -- waiting up to 40s @14:02:22.09 Intake @47.9psia, vacuum from 20.0 to 24.0psi @14:02:22.10 SS.reconfigure forSampling @14:02:22.13 SS.seek 25.00ml @14:02:23.02 Vacuum too high -- waiting up to 40s @14:02:23.28 Intake @48.4psia, vacuum from 20.0 to 24.0psi @14:02:23.29 SS.reconfigure forSampling @14:02:23.32 SS.seek 25.00ml @14:02:24.17 Vacuum too high -- waiting up to 40s @14:02:24.43 Intake @48.1psia, vacuum from 20.0 to 24.0psi @14:02:24.44 SS.reconfigure forSampling @14:02:24.48 SS.seek 25.00ml @14:02:25.30 Vacuum too high -- waiting up to 40s @14:02:25.56 Intake @47.7psia, vacuum from 20.0 to 24.0psi @14:02:25.57 SS.reconfigure forSampling @14:02:25.60 SS.seek 25.00ml @14:02:26.52 Vacuum too high -- waiting up to 40s @14:02:26.78 Intake @47.8psia, vacuum from 20.0 to 24.0psi @14:02:26.79 SS.reconfigure forSampling @14:02:26.82 SS.seek 25.00ml @14:02:27.67 Vacuum too high -- waiting up to 40s @14:02:27.93 Intake @47.5psia, vacuum from 20.0 to 24.0psi @14:02:27.94 SS.reconfigure forSampling @14:02:27.97 SS.seek 25.00ml @14:02:28.77 Vacuum too high -- waiting up to 40s @14:02:29.04 SS.seek 25.00ml @14:02:29.89 Vacuum too high -- waiting up to 40s @14:02:30.15 Intake @47.7psia, vacuum from 20.0 to 24.0psi @14:02:30.16 SS.reconfigure forSampling @14:02:30.20 SS.seek 25.00ml @14:02:31.55 Vacuum too high -- waiting up to 40s @14:02:31.82 Intake @47.7psia, vacuum from 20.0 to 24.0psi @14:02:31.83 SS.reconfigure forSampling @14:02:31.87 SS.seek 25.00ml @14:02:33.11 Vacuum too high -- waiting up to 40s @14:02:33.37 Intake @47.9psia, vacuum from 20.0 to 24.0psi @14:02:33.38 SS.reconfigure forSampling @14:02:33.41 SS.seek 25.00ml @14:02:34.36 Vacuum too high -- waiting up to 40s @14:02:34.62 Intake @48.3psia, vacuum from 20.0 to 24.0psi @14:02:34.63 SS.reconfigure forSampling @14:02:34.66 SS.seek 25.00ml @14:02:35.52 Vacuum too high -- waiting up to 40s @14:02:35.78 Intake @48.2psia, vacuum from 20.0 to 24.0psi @14:02:35.79 SS.reconfigure forSampling @14:02:35.82 SS.seek 25.00ml @14:02:36.67 Vacuum too high -- waiting up to 40s @14:02:36.93 Intake @48.0psia, vacuum from 20.0 to 24.0psi @14:02:36.94 SS.reconfigure forSampling @14:02:36.98 SS.seek 25.00ml @14:02:37.77 Vacuum too high -- waiting up to 40s @14:02:38.03 Intake @47.6psia, vacuum from 20.0 to 24.0psi @14:02:38.04 SS.reconfigure forSampling @14:02:38.07 SS.seek 25.00ml @14:02:39.02 Vacuum too high -- waiting up to 40s @14:02:39.28 Intake @47.7psia, vacuum from 20.0 to 24.0psi @14:02:39.29 SS.reconfigure forSampling @14:02:39.32 SS.seek 25.00ml @14:02:40.21 Vacuum too high -- waiting up to 40s @14:02:40.47 Intake @47.9psia, vacuum from 20.0 to 24.0psi @14:02:40.48 SS.reconfigure forSampling @14:02:40.52 SS.seek 25.00ml @14:02:41.46 Vacuum too high -- waiting up to 40s @14:02:41.72 Intake @48.2psia, vacuum from 20.0 to 24.0psi @14:02:41.73 SS.reconfigure forSampling @14:02:41.76 SS.seek 25.00ml @14:02:42.52 Vacuum too high -- waiting up to 40s @14:02:42.78 Intake @48.1psia, vacuum from 20.0 to 24.0psi @14:02:42.79 SS.reconfigure forSampling @14:02:42.82 SS.seek 25.00ml @14:02:43.61 Vacuum too high -- waiting up to 40s @14:02:43.87 Intake @48.0psia, vacuum from 20.0 to 24.0psi @14:02:43.88 SS.reconfigure forSampling @14:02:43.91 SS.seek 25.00ml @14:02:44.77 Vacuum too high -- waiting up to 40s @14:02:45.03 Intake @47.7psia, vacuum from 20.0 to 24.0psi @14:02:45.04 SS.reconfigure forSampling @14:02:45.07 SS.seek 25.00ml @14:02:45.96 Vacuum too high -- waiting up to 40s @14:02:46.22 Intake @47.6psia, vacuum from 20.0 to 24.0psi @14:02:46.23 SS.reconfigure forSampling @14:02:46.26 SS.seek 25.00ml @14:02:47.11 Vacuum too high -- waiting up to 40s @14:02:47.37 Intake @47.6psia, vacuum from 20.0 to 24.0psi @14:02:47.38 SS.reconfigure forSampling @14:02:47.41 SS.seek 25.00ml @14:02:48.17 Vacuum too high -- waiting up to 40s @14:02:48.43 Intake @47.7psia, vacuum from 20.0 to 24.0psi @14:02:48.44 SS.reconfigure forSampling @14:02:48.47 SS.seek 25.00ml @14:02:49.27 Vacuum too high -- waiting up to 40s @14:02:49.53 SS.seek 25.00ml @14:02:50.33 Vacuum too high -- waiting up to 40s @14:02:50.59 Intake @47.8psia, vacuum from 20.0 to 24.0psi @14:02:50.60 SS.reconfigure forSampling @14:02:50.63 SS.seek 25.00ml @14:02:51.49 Vacuum too high -- waiting up to 40s @14:02:51.75 Intake @48.4psia, vacuum from 20.0 to 24.0psi @14:02:51.76 SS.reconfigure forSampling @14:02:51.79 SS.seek 25.00ml @14:02:52.61 Vacuum too high -- waiting up to 40s @14:02:52.87 Intake @48.5psia, vacuum from 20.0 to 24.0psi @14:02:52.88 SS.reconfigure forSampling @14:02:52.91 SS.seek 25.00ml @14:02:53.74 Vacuum too high -- waiting up to 40s @14:02:54.00 Intake @47.9psia, vacuum from 20.0 to 24.0psi @14:02:54.01 SS.reconfigure forSampling @14:02:54.04 SS.seek 25.00ml @14:02:54.86 Vacuum too high -- waiting up to 40s @14:02:55.12 Intake @47.8psia, vacuum from 20.0 to 24.0psi @14:02:55.13 SS.reconfigure forSampling @14:02:55.17 SS.seek 25.00ml @14:02:56.02 Vacuum too high -- waiting up to 40s @14:02:56.28 Intake @47.6psia, vacuum from 20.0 to 24.0psi @14:02:56.29 SS.reconfigure forSampling @14:02:56.32 SS.seek 25.00ml @14:02:57.14 Vacuum too high -- waiting up to 40s @14:02:57.40 Intake @47.7psia, vacuum from 20.0 to 24.0psi @14:02:57.41 SS.reconfigure forSampling @14:02:57.44 SS.seek 25.00ml @14:02:58.27 Vacuum too high -- waiting up to 40s @14:02:58.53 Intake @47.4psia, vacuum from 20.0 to 24.0psi @14:02:58.54 SS.reconfigure forSampling @14:02:58.57 SS.seek 25.00ml @14:02:59.42 Vacuum too high -- waiting up to 40s @14:02:59.69 SS.seek 25.00ml @14:03:01.33 Vacuum too high -- waiting up to 40s @14:03:01.59 Intake @47.5psia, vacuum from 20.0 to 24.0psi @14:03:01.60 SS.reconfigure forSampling @14:03:01.63 SS.seek 25.00ml @14:03:02.42 Vacuum too high -- waiting up to 40s @14:03:02.68 Intake @47.4psia, vacuum from 20.0 to 24.0psi @14:03:02.69 SS.reconfigure forSampling @14:03:02.72 SS.seek 25.00ml @14:03:03.58 Vacuum too high -- waiting up to 40s @14:03:03.85 SS.seek 25.00ml @14:03:04.66 Vacuum too high -- waiting up to 40s @14:03:04.93 Intake @47.6psia, vacuum from 20.0 to 24.0psi @14:03:04.94 SS.reconfigure forSampling @14:03:04.98 SS.seek 25.00ml @14:03:05.89 Vacuum too high -- waiting up to 40s @14:03:06.15 Intake @48.4psia, vacuum from 20.0 to 24.0psi @14:03:06.16 SS.reconfigure forSampling @14:03:06.20 SS.seek 25.00ml @14:03:07.02 Vacuum too high -- waiting up to 40s @14:03:07.28 Intake @48.3psia, vacuum from 20.0 to 24.0psi @14:03:07.29 SS.reconfigure forSampling @14:03:07.33 SS.seek 25.00ml @14:03:08.11 Vacuum too high -- waiting up to 40s @14:03:08.37 Intake @47.7psia, vacuum from 20.0 to 24.0psi @14:03:08.38 SS.reconfigure forSampling @14:03:08.41 SS.seek 25.00ml @14:03:09.23 Vacuum too high -- waiting up to 40s @14:03:09.50 Intake @47.7psia, vacuum from 20.0 to 24.0psi @14:03:09.51 SS.reconfigure forSampling @14:03:09.55 SS.seek 25.00ml @14:03:10.42 Vacuum too high -- waiting up to 40s @14:03:10.68 Intake @47.7psia, vacuum from 20.0 to 24.0psi @14:03:10.69 SS.reconfigure forSampling @14:03:10.72 SS.seek 25.00ml @14:03:11.55 Vacuum too high -- waiting up to 40s @14:03:11.82 SS.seek 25.00ml @14:03:12.61 Vacuum too high -- waiting up to 40s @14:03:12.87 Intake @47.6psia, vacuum from 20.0 to 24.0psi @14:03:12.88 SS.reconfigure forSampling @14:03:12.91 SS.seek 25.00ml @14:03:13.73 Vacuum too high -- waiting up to 40s @14:03:13.99 Intake @47.8psia, vacuum from 20.0 to 24.0psi @14:03:14.00 SS.reconfigure forSampling @14:03:14.03 SS.seek 25.00ml @14:03:14.89 Vacuum too high -- waiting up to 40s @14:03:15.15 Intake @47.6psia, vacuum from 20.0 to 24.0psi @14:03:15.16 SS.reconfigure forSampling @14:03:15.20 SS.seek 25.00ml @14:03:16.02 Vacuum too high -- waiting up to 40s @14:03:16.29 SS.seek 25.00ml @14:03:17.11 Vacuum too high -- waiting up to 40s @14:03:17.37 Intake @47.6psia, vacuum from 20.0 to 24.0psi @14:03:17.38 SS.reconfigure forSampling @14:03:17.41 SS.seek 25.00ml @14:03:18.20 Vacuum too high -- waiting up to 40s @14:03:18.46 Intake @47.6psia, vacuum from 20.0 to 24.0psi @14:03:18.47 SS.reconfigure forSampling @14:03:18.50 SS.seek 25.00ml @14:03:19.33 Vacuum too high -- waiting up to 40s @14:03:19.59 Intake @47.6psia, vacuum from 20.0 to 24.0psi @14:03:19.60 SS.reconfigure forSampling @14:03:19.63 SS.seek 25.00ml @14:03:20.44 Vacuum too high -- waiting up to 40s @14:03:20.71 Intake @47.6psia, vacuum from 20.0 to 24.0psi @14:03:20.72 SS.reconfigure forSampling @14:03:20.75 SS.seek 25.00ml @14:03:21.52 Vacuum too high -- waiting up to 40s @14:03:21.78 Intake @48.2psia, vacuum from 20.0 to 24.0psi @14:03:21.79 SS.reconfigure forSampling @14:03:21.82 SS.seek 25.00ml @14:03:22.58 Vacuum too high -- waiting up to 40s @14:03:22.84 Intake @48.4psia, vacuum from 20.0 to 24.0psi @14:03:22.85 SS.reconfigure forSampling @14:03:22.88 SS.seek 25.00ml @14:03:23.64 Vacuum too high -- waiting up to 40s @14:03:23.90 Intake @47.9psia, vacuum from 20.0 to 24.0psi @14:03:23.91 SS.reconfigure forSampling @14:03:23.94 SS.seek 25.00ml @14:03:24.73 Vacuum too high -- waiting up to 40s @14:03:24.98 Intake @47.6psia, vacuum from 20.0 to 24.0psi @14:03:24.99 SS.reconfigure forSampling @14:03:25.03 SS.seek 25.00ml @14:03:25.83 Vacuum too high -- waiting up to 40s @14:03:26.09 Intake @47.4psia, vacuum from 20.0 to 24.0psi @14:03:26.10 SS.reconfigure forSampling @14:03:26.13 SS.seek 25.00ml @14:03:26.89 Vacuum too high -- waiting up to 40s @14:03:27.15 Intake @47.4psia, vacuum from 20.0 to 24.0psi @14:03:27.16 SS.reconfigure forSampling @14:03:27.19 SS.seek 25.00ml @14:03:28.08 Vacuum too high -- waiting up to 40s @14:03:28.34 Intake @47.4psia, vacuum from 20.0 to 24.0psi @14:03:28.35 SS.reconfigure forSampling @14:03:28.38 SS.seek 25.00ml @14:03:29.98 Vacuum too high -- waiting up to 40s @14:03:30.24 Intake @47.6psia, vacuum from 20.0 to 24.0psi @14:03:30.25 SS.reconfigure forSampling @14:03:30.29 SS.seek 25.00ml @14:03:31.11 Vacuum too high -- waiting up to 40s @14:03:31.37 Intake @47.7psia, vacuum from 20.0 to 24.0psi @14:03:31.38 SS.reconfigure forSampling @14:03:31.42 SS.seek 25.00ml @14:03:32.20 Vacuum too high -- waiting up to 40s @14:03:32.46 Intake @47.6psia, vacuum from 20.0 to 24.0psi @14:03:32.47 SS.reconfigure forSampling @14:03:32.51 SS.seek 25.00ml @14:03:33.39 Vacuum too high -- waiting up to 40s @14:03:33.65 Intake @47.8psia, vacuum from 20.0 to 24.0psi @14:03:33.66 SS.reconfigure forSampling @14:03:33.69 SS.seek 25.00ml @14:03:34.48 Vacuum too high -- waiting up to 40s @14:03:34.75 SS.seek 25.00ml @14:03:35.39 Vacuum too high -- waiting up to 40s @14:03:35.65 SS.seek 25.00ml @14:03:36.39 Vacuum too high -- waiting up to 40s @14:03:36.73 Intake @48.0psia, vacuum from 20.0 to 24.0psi @14:03:36.74 SS.reconfigure forSampling @14:03:36.78 SS.seek 25.00ml @14:03:37.67 Vacuum too high -- waiting up to 40s @14:03:37.93 Intake @48.0psia, vacuum from 20.0 to 24.0psi @14:03:37.94 SS.reconfigure forSampling @14:03:37.97 SS.seek 25.00ml @14:03:38.76 Vacuum too high -- waiting up to 40s @14:03:39.03 SS.seek 25.00ml @14:03:39.70 Vacuum too high -- waiting up to 40s @14:03:39.96 Intake @48.1psia, vacuum from 20.0 to 24.0psi @14:03:39.97 SS.reconfigure forSampling @14:03:40.00 SS.seek 25.00ml @14:03:40.81 Vacuum too high -- waiting up to 40s @14:03:41.08 Intake @48.0psia, vacuum from 20.0 to 24.0psi @14:03:41.09 SS.reconfigure forSampling @14:03:41.13 SS.seek 25.00ml @14:03:41.86 Vacuum too high -- waiting up to 40s @14:03:42.12 Intake @47.8psia, vacuum from 20.0 to 24.0psi @14:03:42.13 SS.reconfigure forSampling @14:03:42.17 SS.seek 25.00ml @14:03:42.98 Vacuum too high -- waiting up to 40s @14:03:43.24 Intake @47.9psia, vacuum from 20.0 to 24.0psi @14:03:43.25 SS.reconfigure forSampling @14:03:43.28 SS.seek 25.00ml @14:03:44.01 Vacuum too high -- waiting up to 40s @14:03:44.27 SS.seek 25.00ml @14:03:44.98 Vacuum too high -- waiting up to 40s @14:03:45.25 Intake @47.6psia, vacuum from 20.0 to 24.0psi @14:03:45.26 SS.reconfigure forSampling @14:03:45.29 SS.seek 25.00ml @14:03:46.05 Vacuum too high -- waiting up to 40s @14:03:46.31 Intake @47.4psia, vacuum from 20.0 to 24.0psi @14:03:46.32 SS.reconfigure forSampling @14:03:46.35 SS.seek 25.00ml @14:03:47.17 Vacuum too high -- waiting up to 40s @14:03:47.42 Intake @47.3psia, vacuum from 20.0 to 24.0psi @14:03:47.43 SS.reconfigure forSampling @14:03:47.47 SS.seek 25.00ml @14:03:48.32 Vacuum too high -- waiting up to 40s @14:03:48.59 Intake @47.5psia, vacuum from 20.0 to 24.0psi @14:03:48.60 SS.reconfigure forSampling @14:03:48.63 SS.seek 25.00ml @14:03:49.45 Vacuum too high -- waiting up to 40s @14:03:49.71 Intake @48.4psia, vacuum from 20.0 to 24.0psi @14:03:49.72 SS.reconfigure forSampling @14:03:49.75 SS.seek 25.00ml @14:03:50.51 Vacuum too high -- waiting up to 40s @14:03:50.78 Intake @48.5psia, vacuum from 20.0 to 24.0psi @14:03:50.79 SS.reconfigure forSampling @14:03:50.82 SS.seek 25.00ml @14:03:51.55 Vacuum too high -- waiting up to 40s @14:03:51.81 Intake @47.9psia, vacuum from 20.0 to 24.0psi @14:03:51.82 SS.reconfigure forSampling @14:03:51.85 SS.seek 25.00ml @14:03:52.64 Vacuum too high -- waiting up to 40s @14:03:52.90 Intake @47.6psia, vacuum from 20.0 to 24.0psi @14:03:52.91 SS.reconfigure forSampling @14:03:52.94 SS.seek 25.00ml @14:03:53.67 Vacuum too high -- waiting up to 40s @14:03:53.93 Intake @47.7psia, vacuum from 20.0 to 24.0psi @14:03:53.94 SS.reconfigure forSampling @14:03:53.97 SS.seek 25.00ml @14:03:54.76 Vacuum too high -- waiting up to 40s @14:03:55.03 Intake @47.6psia, vacuum from 20.0 to 24.0psi @14:03:55.04 SS.reconfigure forSampling @14:03:55.07 SS.seek 25.00ml @14:03:55.83 Vacuum too high -- waiting up to 40s @14:03:56.09 Intake @47.5psia, vacuum from 20.0 to 24.0psi @14:03:56.10 SS.reconfigure forSampling @14:03:56.13 SS.seek 25.00ml @14:03:57.76 Vacuum too high -- waiting up to 40s @14:03:58.03 SS.seek 25.00ml @14:03:58.73 Vacuum too high -- waiting up to 40s @14:03:58.99 Intake @47.4psia, vacuum from 20.0 to 24.0psi @14:03:59.00 SS.reconfigure forSampling @14:03:59.04 SS.seek 25.00ml @14:03:59.89 Vacuum too high -- waiting up to 40s @14:04:00.16 Intake @47.2psia, vacuum from 20.0 to 24.0psi @14:04:00.17 SS.reconfigure forSampling @14:04:00.20 SS.seek 25.00ml @14:04:01.08 Vacuum too high -- waiting up to 40s @14:04:01.34 Intake @47.3psia, vacuum from 20.0 to 24.0psi @14:04:01.35 SS.reconfigure forSampling @14:04:01.38 SS.seek 25.00ml @14:04:02.17 Vacuum too high -- waiting up to 40s @14:04:02.64 Intake @47.6psia, vacuum from 20.0 to 24.0psi @14:04:02.65 SS.reconfigure forSampling @14:04:02.68 SS.seek 25.00ml @14:04:03.61 Vacuum too high -- waiting up to 40s @14:04:03.87 Intake @48.4psia, vacuum from 20.0 to 24.0psi @14:04:03.88 SS.reconfigure forSampling @14:04:03.91 SS.seek 25.00ml @14:04:04.67 Vacuum too high -- waiting up to 40s @14:04:04.93 Intake @48.4psia, vacuum from 20.0 to 24.0psi @14:04:04.94 SS.reconfigure forSampling @14:04:04.97 SS.seek 25.00ml @14:04:05.70 Vacuum too high -- waiting up to 40s @14:04:05.96 Intake @47.9psia, vacuum from 20.0 to 24.0psi @14:04:05.97 SS.reconfigure forSampling @14:04:06.00 SS.seek 25.00ml @14:04:06.79 Vacuum too high -- waiting up to 40s @14:04:07.05 Intake @47.8psia, vacuum from 20.0 to 24.0psi @14:04:07.06 SS.reconfigure forSampling @14:04:07.09 SS.seek 25.00ml @14:04:07.79 Vacuum too high -- waiting up to 40s @14:04:08.05 Intake @47.6psia, vacuum from 20.0 to 24.0psi @14:04:08.06 SS.reconfigure forSampling @14:04:08.09 SS.seek 25.00ml @14:04:08.86 Vacuum too high -- waiting up to 40s @14:04:09.33 Intake @47.6psia, vacuum from 20.0 to 24.0psi @14:04:09.34 SS.reconfigure forSampling @14:04:09.37 SS.seek 25.00ml @14:04:10.29 Vacuum too high -- waiting up to 40s @14:04:10.55 Intake @47.4psia, vacuum from 20.0 to 24.0psi @14:04:10.56 SS.reconfigure forSampling @14:04:10.59 SS.seek 25.00ml @14:04:11.39 Vacuum too high -- waiting up to 40s @14:04:11.65 Intake @47.3psia, vacuum from 20.0 to 24.0psi @14:04:11.66 SS.reconfigure forSampling @14:04:11.70 SS.seek 25.00ml @14:04:12.48 Vacuum too high -- waiting up to 40s @14:04:12.74 Intake @47.4psia, vacuum from 20.0 to 24.0psi @14:04:12.75 SS.reconfigure forSampling @14:04:12.78 SS.seek 25.00ml @14:04:13.51 Vacuum too high -- waiting up to 40s @14:04:13.77 Intake @47.2psia, vacuum from 20.0 to 24.0psi @14:04:13.78 SS.reconfigure forSampling @14:04:13.81 SS.seek 25.00ml @14:04:14.67 Vacuum too high -- waiting up to 40s @14:04:15.15 Intake @47.1psia, vacuum from 20.0 to 24.0psi @14:04:15.16 SS.reconfigure forSampling @14:04:15.20 SS.seek 25.00ml @14:04:16.17 Vacuum too high -- waiting up to 40s @14:04:16.65 Intake @47.1psia, vacuum from 20.0 to 24.0psi @14:04:16.66 SS.reconfigure forSampling @14:04:16.69 SS.seek 25.00ml @14:04:17.70 Vacuum too high -- waiting up to 40s @14:04:18.17 Intake @47.3psia, vacuum from 20.0 to 24.0psi @14:04:18.18 SS.reconfigure forSampling @14:04:18.21 SS.seek 25.00ml @14:04:19.23 Vacuum too high -- waiting up to 40s @14:04:19.70 Intake @48.4psia, vacuum from 20.0 to 24.0psi @14:04:19.71 SS.reconfigure forSampling @14:04:19.74 SS.seek 25.00ml @14:04:20.67 Vacuum too high -- waiting up to 40s @14:04:20.93 Intake @47.9psia, vacuum from 20.0 to 24.0psi @14:04:20.94 SS.reconfigure forSampling @14:04:20.97 SS.seek 25.00ml @14:04:21.73 Vacuum too high -- waiting up to 40s @14:04:21.99 Intake @47.5psia, vacuum from 20.0 to 24.0psi @14:04:22.00 SS.reconfigure forSampling @14:04:22.03 SS.seek 25.00ml @14:04:22.82 Vacuum too high -- waiting up to 40s @14:04:23.29 Intake @47.3psia, vacuum from 20.0 to 24.0psi @14:04:23.30 SS.reconfigure forSampling @14:04:23.33 SS.seek 25.00ml @14:04:24.32 Vacuum too high -- waiting up to 40s @14:04:24.79 Intake @47.4psia, vacuum from 20.0 to 24.0psi @14:04:25.31 SS.reconfigure forSampling @14:04:25.34 SS.seek 25.00ml @14:04:26.54 Vacuum too high -- waiting up to 40s @14:04:27.01 Intake @47.4psia, vacuum from 20.0 to 24.0psi @14:04:27.02 SS.reconfigure forSampling @14:04:27.05 SS.seek 25.00ml @14:04:28.07 Vacuum too high -- waiting up to 40s @14:04:28.54 Intake @47.6psia, vacuum from 20.0 to 24.0psi @14:04:28.55 SS.reconfigure forSampling @14:04:28.58 SS.seek 25.00ml @14:04:29.54 Vacuum too high -- waiting up to 40s @14:04:30.01 Intake @47.3psia, vacuum from 20.0 to 24.0psi @14:04:30.02 SS.reconfigure forSampling @14:04:30.05 SS.seek 25.00ml @14:04:31.04 Vacuum too high -- waiting up to 40s @14:04:31.51 Intake @47.7psia, vacuum from 20.0 to 24.0psi @14:04:31.52 SS.reconfigure forSampling @14:04:31.55 SS.seek 25.00ml @14:04:32.51 Vacuum too high -- waiting up to 40s @14:04:32.77 Intake @47.6psia, vacuum from 20.0 to 24.0psi @14:04:32.78 SS.reconfigure forSampling @14:04:32.81 SS.seek 25.00ml @14:04:33.57 Vacuum too high -- waiting up to 40s @14:04:33.83 Intake @47.5psia, vacuum from 20.0 to 24.0psi @14:04:33.84 SS.reconfigure forSampling @14:04:33.87 SS.seek 25.00ml @14:04:34.76 Vacuum too high -- waiting up to 40s @14:04:35.23 Intake @47.7psia, vacuum from 20.0 to 24.0psi @14:04:35.24 SS.reconfigure forSampling @14:04:35.27 SS.seek 25.00ml @14:04:36.23 Vacuum too high -- waiting up to 40s @14:04:36.70 Intake @47.9psia, vacuum from 20.0 to 24.0psi @14:04:36.71 SS.reconfigure forSampling @14:04:36.74 SS.seek 25.00ml @14:04:37.64 Vacuum too high -- waiting up to 40s @14:04:38.13 Intake @47.9psia, vacuum from 20.0 to 24.0psi @14:04:38.14 SS.reconfigure forSampling @14:04:38.17 SS.seek 25.00ml @14:04:39.20 Vacuum too high -- waiting up to 40s @14:04:39.67 Intake @48.2psia, vacuum from 20.0 to 24.0psi @14:04:39.68 SS.reconfigure forSampling @14:04:39.71 SS.seek 25.00ml @14:04:40.57 Vacuum too high -- waiting up to 40s @14:04:40.83 Intake @47.8psia, vacuum from 20.0 to 24.0psi @14:04:40.84 SS.reconfigure forSampling @14:04:40.87 SS.seek 25.00ml @14:04:41.67 Vacuum too high -- waiting up to 40s @14:04:42.15 Intake @47.6psia, vacuum from 20.0 to 24.0psi @14:04:42.16 SS.reconfigure forSampling @14:04:42.19 SS.seek 25.00ml @14:04:43.17 Vacuum too high -- waiting up to 40s @14:04:43.65 Intake @47.6psia, vacuum from 20.0 to 24.0psi @14:04:43.66 SS.reconfigure forSampling @14:04:43.69 SS.seek 25.00ml @14:04:44.74 Vacuum too high -- waiting up to 40s @14:04:45.03 Intake @47.6psia, vacuum from 20.0 to 24.0psi @14:04:45.04 SS.reconfigure forSampling @14:04:45.08 SS.seek 25.00ml @14:04:45.92 Vacuum too high -- waiting up to 40s @14:04:46.18 Intake @47.5psia, vacuum from 20.0 to 24.0psi @14:04:46.19 SS.reconfigure forSampling @14:04:46.22 SS.seek 25.00ml @14:04:46.98 Vacuum too high -- waiting up to 40s @14:04:47.45 Intake @47.5psia, vacuum from 20.0 to 24.0psi @14:04:47.46 SS.reconfigure forSampling @14:04:47.49 SS.seek 25.00ml @14:04:48.48 Vacuum too high -- waiting up to 40s @14:04:48.95 Intake @47.3psia, vacuum from 20.0 to 24.0psi @14:04:48.96 SS.reconfigure forSampling @14:04:48.99 SS.seek 25.00ml @14:04:50.07 Vacuum too high -- waiting up to 40s @14:04:50.55 Intake @48.3psia, vacuum from 20.0 to 24.0psi @14:04:50.56 SS.reconfigure forSampling @14:04:50.59 SS.seek 25.00ml @14:04:51.48 Vacuum too high -- waiting up to 40s @14:04:51.74 Intake @48.4psia, vacuum from 20.0 to 24.0psi @14:04:51.75 SS.reconfigure forSampling @14:04:51.78 SS.seek 25.00ml @14:04:52.48 Vacuum too high -- waiting up to 40s @14:04:52.74 Intake @47.8psia, vacuum from 20.0 to 24.0psi @14:04:52.75 SS.reconfigure forSampling @14:04:52.78 SS.seek 25.00ml @14:04:53.57 Vacuum too high -- waiting up to 40s @14:04:54.04 Intake @47.5psia, vacuum from 20.0 to 24.0psi @14:04:54.53 SS.reconfigure forSampling @14:04:54.57 SS.seek 25.00ml @14:04:56.00 Vacuum too high -- waiting up to 40s @14:04:56.26 Intake @47.6psia, vacuum from 20.0 to 24.0psi @14:04:56.27 SS.reconfigure forSampling @14:04:56.30 SS.seek 25.00ml @14:04:57.29 Vacuum too high -- waiting up to 40s @14:04:57.77 Intake @47.5psia, vacuum from 20.0 to 24.0psi @14:04:57.78 SS.reconfigure forSampling @14:04:57.81 SS.seek 25.00ml @14:04:58.70 Vacuum too high -- waiting up to 40s @14:04:59.18 Intake @47.6psia, vacuum from 20.0 to 24.0psi @14:04:59.19 SS.reconfigure forSampling @14:04:59.23 SS.seek 25.00ml @14:05:00.14 Vacuum too high -- waiting up to 40s @14:05:00.61 Intake @47.3psia, vacuum from 20.0 to 24.0psi @14:05:00.62 SS.reconfigure forSampling @14:05:00.66 SS.seek 25.00ml @14:05:01.60 Vacuum too high -- waiting up to 40s @14:05:02.07 Intake @47.3psia, vacuum from 20.0 to 24.0psi @14:05:02.08 SS.reconfigure forSampling @14:05:02.11 SS.seek 25.00ml @14:05:03.10 Vacuum too high -- waiting up to 40s @14:05:03.57 Intake @47.8psia, vacuum from 20.0 to 24.0psi @14:05:03.58 SS.reconfigure forSampling @14:05:03.61 SS.seek 25.00ml @14:05:04.51 Vacuum too high -- waiting up to 40s @14:05:04.98 Intake @48.3psia, vacuum from 20.0 to 24.0psi @14:05:04.99 SS.reconfigure forSampling @14:05:05.02 SS.seek 25.00ml @14:05:05.85 Vacuum too high -- waiting up to 40s @14:05:06.11 Intake @48.1psia, vacuum from 20.0 to 24.0psi @14:05:06.12 SS.reconfigure forSampling @14:05:06.15 SS.seek 25.00ml @14:05:06.82 Vacuum too high -- waiting up to 40s @14:05:07.08 Intake @48.2psia, vacuum from 20.0 to 24.0psi @14:05:07.09 SS.reconfigure forSampling @14:05:07.12 SS.seek 25.00ml @14:05:07.85 Vacuum too high -- waiting up to 40s @14:05:08.32 Intake @48.1psia, vacuum from 20.0 to 24.0psi @14:05:08.33 SS.reconfigure forSampling @14:05:08.36 SS.seek 25.00ml @14:05:09.26 Vacuum too high -- waiting up to 40s @14:05:09.52 Intake @47.7psia, vacuum from 20.0 to 24.0psi @14:05:09.53 SS.reconfigure forSampling @14:05:09.56 SS.seek 25.00ml @14:05:10.35 Vacuum too high -- waiting up to 40s @14:05:10.82 Intake @47.5psia, vacuum from 20.0 to 24.0psi @14:05:10.83 SS.reconfigure forSampling @14:05:10.86 SS.seek 25.00ml @14:05:11.79 Vacuum too high -- waiting up to 40s @14:05:12.28 SS.seek 25.00ml @14:05:13.17 Vacuum too high -- waiting up to 40s @14:05:13.65 Intake @47.5psia, vacuum from 20.0 to 24.0psi @14:05:13.66 SS.reconfigure forSampling @14:05:13.69 SS.seek 25.00ml @14:05:14.60 Vacuum too high -- waiting up to 40s @14:05:15.08 Intake @47.4psia, vacuum from 20.0 to 24.0psi @14:05:15.09 SS.reconfigure forSampling @14:05:15.12 SS.seek 25.00ml @14:05:16.07 Vacuum too high -- waiting up to 40s @14:05:16.53 Intake @47.3psia, vacuum from 20.0 to 24.0psi @14:05:16.54 SS.reconfigure forSampling @14:05:16.58 SS.seek 25.00ml @14:05:17.60 Vacuum too high -- waiting up to 40s @14:05:18.07 Intake @47.9psia, vacuum from 20.0 to 24.0psi @14:05:18.08 SS.reconfigure forSampling @14:05:18.11 SS.seek 25.00ml @14:05:19.01 Vacuum too high -- waiting up to 40s @14:05:19.48 Intake @48.2psia, vacuum from 20.0 to 24.0psi @14:05:19.49 SS.reconfigure forSampling @14:05:19.52 SS.seek 25.00ml @14:05:20.42 Vacuum too high -- waiting up to 40s @14:05:20.68 Intake @47.9psia, vacuum from 20.0 to 24.0psi @14:05:20.69 SS.reconfigure forSampling @14:05:20.72 SS.seek 25.00ml @14:05:21.45 Vacuum too high -- waiting up to 40s @14:05:21.74 SS.reconfigure SSconfig @14:05:21.78 SS.hold @14:05:21.87 Intake.select :close @14:05:22.01 ShallowSampler::Clogged in MAIN -- progress too slow after 498.7ml SSV.dial :exhaust @14:05:22.82 Exhaust.select :open @14:05:23.44 SS.reconfigure SSexhale @14:05:23.48 SS.seek 0.00ml @14:05:31.80 Exhaust.select :close @14:05:31.90 Sampled 498.7ml @14:05:31.91 collectSample: Complete Evacuating Puck (bubblePt=55.0psi, delay=15.0s) @14:05:31.92 Intake.select :close @14:05:31.98 CSV.dial :waste @14:05:32.01 CS.seek 0.000ml @14:05:32.11 SSV.dial CBV,avoiding => exhaust @14:05:32.13
CBV.dial SSV @14:05:32.16 CSV.dial :air @14:05:33.00 CS.seek 10.000ml @14:05:34.12
CTV.dial :air @14:05:34.90 delay 15 seconds @14:05:34.91 Sleepy.power :sampler,:OFF @14:05:49.90
SSV.dial :spit,avoiding => exhaust @14:05:51.23 CTV.dial CSV,avoiding => intake @14:05:51.25
SS.seek 0.00ml @14:05:51.35 SSV.dial CBV,avoiding => exhaust @14:05:57.71 CBV.dial between CSV and waste2b @14:05:58.65 CTV.dial CSV,avoiding => air @14:05:58.67
CSV.dial CTV @14:05:59.12 CS.reconfigure CSpressurize @14:05:59.15 CS.seek 0.000ml @14:06:22.98 BubblePoint: Intake @70.0psia, Outlet @21.4psia @14:06:22.99 SS.reconfigure SSconfig @14:06:23.03 SS.seek 10.00ml @14:06:29.00 delay 15 seconds @14:06:44.10 Evacuated: Intake @69.4psia, Outlet @-0.4psia @14:06:44.11 SSV.dial :spit,avoiding => exhaust @14:06:45.43 SS.reconfigure SSexhale @14:06:45.46 SS.seek 0.00ml @14:06:50.51 SS.reconfigure SSfast @14:06:50.55 SS.seek 5.00ml @14:06:53.60 SSV.dial CBV,avoiding => exhaust @14:06:53.62
CBV.dial SSV @14:06:54.95 CTV.dial :air,avoiding => intake @14:06:56.25 CSV.dial CTV @14:06:56.27
SS.reconfigure SSconfig @14:06:56.31 SS.seek 10.00ml @14:06:57.27 CSV.dial :air @14:06:57.79 CS.reconfigure CSfast @14:06:57.82 CS.seek 0.000ml @14:06:59.37
SSV.dial :spit,avoiding => exhaust @14:07:00.44 CS.coast @14:07:00.68
SS.reconfigure SSexhale @14:07:00.71 SS.seek 0.00ml @14:07:05.89 SS.reconfigure SSfast @14:07:05.92 SS.seek 5.00ml @14:07:08.96 SSV.dial CBV,avoiding => exhaust @14:07:08.98
CBV.dial SSV @14:07:11.28 SSV.dial :spit,avoiding => exhaust @14:07:12.59 SS.reconfigure SSconfig @14:07:12.62 SS.seek 0.00ml @14:07:15.92 SS.coast @14:07:15.95 Evacuating Puck (bubblePt=55.0psi, delay=15.0s) Intake.select :close @14:07:16.01 CSV.dial :waste @14:07:16.09
SSV.dial :exhaust @14:07:16.76 CS.seek 0.000ml @14:07:16.83
Exhaust.select :open @14:07:16.93 SS.reconfigure SSexhale @14:07:16.97 SS.seek 0.00ml @14:07:17.03 CSV.dial :air @14:07:17.62
Exhaust.select :close @14:07:17.71 SSV.dial CBV,avoiding => exhaust @14:07:17.73
CBV.dial SSV @14:07:17.84 CS.seek 10.000ml @14:07:19.60
CTV.dial :air @14:07:19.61 delay 15 seconds @14:07:34.61 SSV.dial :spit,avoiding => exhaust @14:07:35.93 CTV.dial CSV,avoiding => intake @14:07:35.95
SS.seek 0.00ml @14:07:36.04 SSV.dial CBV,avoiding => exhaust @14:07:41.80 CBV.dial between CSV and waste2b @14:07:42.72 CSV.dial between air and CTV @14:07:43.02 CS.seek 7.189ml @14:07:50.29 CTV.dial CSV,avoiding => air @14:07:50.30
CSV.dial CTV @14:07:50.59 CS.reconfigure CSpressurize @14:07:50.62 CS.seek 0.000ml @14:08:09.37 CSV.dial :air @14:08:09.90 CS.reconfigure CSfast @14:08:09.93 CS.seek 10.000ml @14:08:33.54 CSV.dial between air and CTV @14:08:33.93 CS.seek 2.138ml @14:08:52.85 CTV.dial CSV,avoiding => air @14:08:52.86
CSV.dial CTV @14:08:53.15 CS.reconfigure CSpressurize @14:08:53.18 CS.seek 0.000ml @14:08:54.73 BubblePoint: Intake @73.7psia, Outlet @18.4psia @14:08:54.74 SS.reconfigure SSconfig @14:08:54.77 SS.seek 10.00ml @14:09:00.77 delay 15 seconds @14:09:16.46 Evacuated: Intake @72.9psia, Outlet @-0.4psia SSV.dial :spit,avoiding => exhaust @14:09:17.77 SS.reconfigure SSexhale @14:09:17.80 SS.seek 0.00ml @14:09:22.76 SS.reconfigure SSfast @14:09:22.80 SS.seek 5.00ml @14:09:25.84 SSV.dial CBV,avoiding => exhaust @14:09:25.86
CBV.dial SSV @14:09:27.19 CTV.dial :air,avoiding => intake @14:09:28.46 CSV.dial CTV @14:09:28.48
SS.reconfigure SSconfig @14:09:28.52 SS.seek 10.00ml @14:09:29.48 CSV.dial :air @14:09:30.21 CS.reconfigure CSfast @14:09:30.24 CS.seek 0.000ml @14:09:31.63
SSV.dial :spit,avoiding => exhaust @14:09:32.95 SS.reconfigure SSexhale @14:09:32.98 SS.seek 0.00ml @14:09:34.90 CS.coast @14:09:38.24
SS.reconfigure SSfast @14:09:38.28 SS.seek 5.00ml @14:09:41.50 SSV.dial CBV,avoiding => exhaust @14:09:41.52
CBV.dial SSV @14:09:43.82 SSV.dial :spit,avoiding => exhaust @14:09:45.14 SS.reconfigure SSconfig @14:09:45.17 SS.seek 0.00ml @14:09:48.24 SS.coast @14:09:48.27 Lysing: Adding 1.5ml lysis CBV.dial between CSV and waste1,avoiding => DBV @14:09:49.74 CTV.dial CSV @14:09:51.01 CSV.dial CSR @14:09:51.71 CSR.select :lysis @14:09:51.81 CS.reconfigure CSconfig @14:09:51.85 CS.seek 1.000ml @14:09:55.21 delay 5 seconds @14:10:00.23 CSR.select :air @14:10:00.37 CS.seek 6.000ml @14:10:13.80 delay 2 seconds @14:10:15.81 CSV.dial :waste1 @14:10:17.06 CS.reconfigure CSfast @14:10:17.09 CS.seek empty @14:10:31.55 CSV.dial CSR @14:10:32.82 CSR.select :lysis @14:10:32.92 CS.reconfigure CSconfig @14:10:32.96 CS.seek 1.500ml @14:10:37.58 delay 5 seconds @14:10:42.60 CSR.select :air @14:10:42.74 CS.seek 4.500ml @14:10:51.12 delay 2 seconds @14:10:53.13 CSV.dial CTV @14:10:53.64 CS.seek 1.275ml @14:11:02.69 Heating sh1 puck from tube 3 in Collection Clamp to 85.0C for 5:00 @14:11:02.70 CH.seek 85.0C,8:00 @14:14:10.56 CH.hold 5:00,85.0C @14:19:10.66 Collection Puck Heater at 85.0C after lysing @14:19:10.68 CS.seek 0.000ml @14:19:14.71 CTV.dial between spare and air,avoiding => intake @14:19:15.68 CSV.dial :air @14:19:16.11 CS.seek 5.000ml @14:19:29.60 CSV.dial CTV @14:19:30.11 delay 2 seconds @14:19:32.11 CSV.dial :air @14:19:32.62 delay 2 seconds @14:19:34.63 CSV.dial CTV @14:19:35.13 delay 2 seconds @14:19:37.14 CSV.dial :air @14:19:37.63 delay 2 seconds @14:19:39.64 CS.seek 0.000ml @14:19:53.13 Recovering Lysate into Collection syringe @14:19:53.14 CSV.dial CBV @14:19:54.13 CBV.dial CSV @14:19:54.49 CS.seek 5.000ml @14:20:07.97 delay 5 seconds @14:20:12.99 CTV.dial :air @14:20:13.48 CS.seek 7.000ml @14:20:19.41 delay 5 seconds @14:20:24.43 CSV.dial :air2 @14:20:24.86 CS.seek 10.000ml @14:20:33.29 CSV.dial between air and CTV,avoiding => CBV @14:20:34.42 Lysing Complete @14:20:34.47 Storing sh1 puck in Collection Clamp to tube 2 @14:20:34.59 SC.reconfigure SCtight @14:20:34.63 SC.seek 2 @14:20:36.85 SC.reconfigure SCloose @14:20:37.02 Elbow.reconfigure TightElbow @14:20:37.06 Elbow.seek 2 @14:20:38.65 Forearm.reconfigure TightForearm @14:20:38.68 Forearm.seek 2 + 800 counts @14:20:39.77 SE.reconfigure SEpick @14:20:39.82 SE.seek beamBreak @14:20:42.11 SE.configure SEconfig @14:20:42.23 Elbow.reconfigure LimpElbow @14:20:42.26 Forearm.reconfigure LooseForearm @14:20:42.30 Forearm.seek 2 @14:20:42.50 Elbow.hold @14:20:42.55 Elbow.reconfigure TightElbow @14:20:42.63 SE.seek 7.03 Pucks below Top Plate @14:20:42.96 Forearm.coast @14:20:42.99 Forearm.seek 2 + 313 counts @14:20:43.77 Forearm.reconfigure TightForearm @14:20:43.80 Forearm.seek 2 + 800 counts @14:20:44.06 SE.seek 8.03 Pucks below Top Plate @14:20:44.59 Forearm.reconfigure LooseForearm @14:20:44.62 Forearm.seek retract @14:20:45.54 Elbow.seek CC @14:20:47.08 Elbow.reconfigure LooseElbow @14:20:47.16 Forearm.seek CC @14:20:48.12 CC.seek sealed @14:20:49.07 Hand.grip @14:20:49.78 CC.seek open @14:20:53.13 Forearm.seek retract @14:20:54.11 Elbow.reconfigure TightElbow @14:20:54.15 Elbow.seek 2 @14:20:55.78 Elbow.reconfigure LimpElbow @14:20:55.82 Forearm.seek 2 @14:20:56.86 Elbow.hold @14:20:56.91 Elbow.reconfigure TightElbow @14:20:56.95 Hand.release @14:20:57.73 Forearm.coast @14:20:57.76 Forearm.seek 2 + 296 counts @14:20:57.97 Forearm.reconfigure TightForearm @14:20:58.01 Forearm.seek 2 + 800 counts @14:20:58.41 SE.seek down @14:20:59.51
Forearm.reconfigure LooseForearm @14:20:59.55 Forearm.seek retract @14:21:00.33 SE.coast @14:21:00.47
SC.coast @14:21:00.50 Tranferring lysate to PRV @14:21:00.51 CSV.dial :air @14:21:00.81 CS.seek 10.000ml @14:21:00.88 CSV.dial between air and CTV @14:21:01.26 AIV.dial :bypass @14:21:01.42 Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:21:01.57 Elbow.seek FlushPuck::Garage @14:21:03.32 Elbow.reconfigure LooseElbow @14:21:03.40 Forearm.seek garage @14:21:04.45 Hand.grip @14:21:05.23 Forearm.seek retract @14:21:06.28 Elbow.reconfigure TightElbow @14:21:06.32 Elbow.seek CC @14:21:09.12 Elbow.reconfigure LooseElbow @14:21:09.21 Forearm.reconfigure TightForearm @14:21:09.24 Forearm.seek CC @14:21:10.36 CC.seek closed @14:21:14.16 Collection Clamp onSpring/offSpring for 5/22 samples, on/off torque 2.02 @14:21:14.21 Hand.release @14:21:14.94 Forearm.reconfigure LooseForearm @14:21:14.97 Forearm.seek retract @14:21:15.92 AWV.select :air @14:21:15.93 AIV.dial :bypass @14:21:15.94 PSV.dial PSR @14:21:17.21 PSR.select :flush @14:21:17.31 PS.seek 1.500ml @14:21:33.39 delay 5 seconds @14:21:38.40 PSV.dial :waste1 @14:21:38.84 PS.seek 0.000ml @14:21:54.89 PSV.dial PSR @14:21:55.34 PSR.select :air @14:21:55.44 PS.seek 2.500ml @14:22:21.68 CBV.dial PRV @14:22:21.69 PRV.dial CBV @14:22:22.45
PSV.dial :mixloop @14:22:23.44 CTV.dial :air,avoiding => intake @14:22:23.45 PTV.dial :PRVmixing @14:22:23.53 PS.seek 0.000ml @14:22:49.76 PSV.dial :air @14:22:50.70 PS.seek 2.500ml @14:23:17.01 PSV.dial :mixloop @14:23:17.46 PS.seek 0.000ml @14:23:43.67 PSV.dial :air @14:23:44.12 PS.seek 2.500ml @14:24:10.29 PSV.dial :mixloop @14:24:10.74 PS.seek 0.000ml @14:24:37.14 Garaging FlushPuck in Collection Clamp @14:24:37.20 Elbow.reconfigure TightElbow @14:24:37.23 Elbow.seek CC @14:24:37.43 Elbow.reconfigure LooseElbow @14:24:37.51 Forearm.seek CC @14:24:38.49 CC.seek sealed @14:24:39.40 Hand.grip @14:24:40.14 CC.seek open @14:24:43.43 Forearm.seek retract @14:24:44.41 Elbow.reconfigure TightElbow @14:24:44.45 Elbow.seek garage @14:24:47.27 Elbow.reconfigure LooseElbow @14:24:47.35 Forearm.reconfigure TightForearm @14:24:47.38 Forearm.seek garage @14:24:48.55 Hand.release @14:24:49.31 Forearm.reconfigure LooseForearm @14:24:49.34 Forearm.seek retract @14:24:50.40 Connecting PS to CS to transfer sample @14:24:50.41 CBV.dial AIV @14:24:50.42 AIV.dial :bypass @14:24:50.43 PSV.dial :mixloop @14:24:50.44 PTV.dial :PRVmixing PRV.dial AIV @14:24:50.45 CTV.dial :air,avoiding => intake @14:24:50.46 Loading lyfil puck from tube 3 into Collection Clamp @14:24:50.64 SC.reconfigure SCtight @14:24:50.67 SC.seek 3 @14:24:52.96 SC.reconfigure SCloose @14:24:53.13 Elbow.reconfigure TightElbow @14:24:53.16 Elbow.seek 3 @14:24:55.03 Forearm.reconfigure TightForearm @14:24:55.06 Forearm.seek 3 + 800 counts @14:24:56.13 SE.reconfigure SEpick @14:24:56.18 SE.seek beamBreak @14:24:57.89 SE.configure SEconfig @14:24:58.01 Elbow.seek 3 @14:24:58.25 Forearm.seek 3 + 300 counts @14:24:58.57 Forearm.reconfigure LooseForearm @14:24:58.61 Hand.grip @14:24:59.39 SE.seek down @14:24:59.54
Forearm.seek retract @14:25:00.48 Elbow.seek CC @14:25:00.96 SE.coast @14:25:02.04
Elbow.reconfigure LooseElbow @14:25:02.12 Forearm.reconfigure TightForearm @14:25:02.15 Forearm.seek CC @14:25:03.26 CC.seek closed @14:25:07.04 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 2.06 @14:25:07.05 Hand.release @14:25:07.81 Forearm.reconfigure LooseForearm @14:25:07.84 Forearm.seek retract @14:25:08.79 SC.coast @14:25:08.82 CTV.dial CSV,avoiding => intake @14:25:10.12 CSV.dial CTV @14:25:10.41 CS.seek 5.000ml @14:25:23.87 delay 1:20 @14:26:43.88 CS.seek 2.500ml @14:26:51.08 delay 40 seconds @14:27:31.09 CS.seek 0.000ml @14:27:38.27 delay 1:00 @14:28:38.28 PSV.dial :air @14:28:38.85 PS.seek 2.500ml @14:29:05.03 PSV.dial :mixloop @14:29:05.45 delay 3 seconds @14:29:08.46 PSV.dial :air @14:29:08.89 delay 2 seconds @14:29:10.90 PSV.dial :mixloop @14:29:11.34 delay 3 seconds @14:29:14.35 PSV.dial :waste1 @14:29:15.62 PS.seek empty @14:29:41.98 CBV.dial :waste1 @14:29:42.44 delay 5 seconds @14:29:47.45 CBV.dial AIV @14:29:48.38 delay 10 seconds @14:29:58.39 CBV.dial :waste1 @14:29:58.86 delay 10 seconds @14:30:08.87 CBV.dial AIV @14:30:09.32 delay 10 seconds @14:30:19.33 CTV.dial CSV,avoiding => intake @14:30:19.34 CBV.dial between AIV and waste1 @14:30:19.68 CSV.dial :air @14:30:20.20 CS.seek 10.000ml @14:30:46.30 CSV.dial CTV @14:30:46.81 CS.seek 0.000ml @14:31:13.11 CBV.dial :waste1 @14:31:13.41 delay 10 seconds @14:31:23.42 CTV.dial :air,avoiding => intake @14:31:24.75 Storing lyfil puck in Collection Clamp to tube 2 @14:31:24.88 SC.reconfigure SCtight @14:31:24.91 SC.seek 2 @14:31:27.05 SC.reconfigure SCloose @14:31:27.22 Elbow.reconfigure TightElbow @14:31:27.26 Elbow.seek 2 @14:31:28.87 Forearm.reconfigure TightForearm @14:31:28.90 Forearm.seek 2 + 800 counts @14:31:30.00 SE.reconfigure SEpick @14:31:30.05 SE.seek beamBreak @14:31:32.23 SE.configure SEconfig @14:31:32.35 Elbow.reconfigure LimpElbow @14:31:32.38 Forearm.reconfigure LooseForearm @14:31:32.42 Forearm.seek 2 @14:31:32.67 Elbow.hold @14:31:32.72 Elbow.reconfigure TightElbow @14:31:32.80 SE.seek 8.03 Pucks below Top Plate @14:31:33.12 Forearm.coast @14:31:33.15 Forearm.seek 2 + 317 counts @14:31:33.44 Forearm.reconfigure TightForearm @14:31:33.47 Forearm.seek 2 + 800 counts @14:31:33.71 SE.seek 9.03 Pucks below Top Plate @14:31:34.24 Forearm.reconfigure LooseForearm @14:31:34.27 Forearm.seek retract @14:31:35.19 Elbow.seek CC @14:31:36.76 Elbow.reconfigure LooseElbow @14:31:36.85 Forearm.seek CC @14:31:37.80 CC.seek sealed @14:31:38.73 Hand.grip @14:31:39.51 CC.seek open @14:31:42.90 Forearm.seek retract @14:31:43.84 Elbow.reconfigure TightElbow @14:31:43.88 Elbow.seek 2 @14:31:45.49 Elbow.reconfigure LimpElbow @14:31:45.53 Forearm.seek 2 @14:31:46.53 Elbow.hold @14:31:46.58 Elbow.reconfigure TightElbow @14:31:46.61 Hand.release @14:31:47.46 Forearm.coast @14:31:47.49 Forearm.seek 2 + 320 counts @14:31:47.72 Forearm.reconfigure TightForearm @14:31:47.75 Forearm.seek 2 + 800 counts @14:31:48.06 SE.seek down @14:31:49.17
Forearm.reconfigure LooseForearm @14:31:49.21 Forearm.seek retract @14:31:49.91 SE.coast @14:31:50.18
SC.coast @14:31:50.21 PSV.dial :air @14:31:51.26 PS.seek 0.499ml @14:31:57.19 delay 3 seconds @14:32:00.20 PSV.dial :mixloop @14:32:00.64 PRV.dial :air @14:32:01.10 delay 3 seconds @14:32:04.11 PRV.dial CBV @14:32:04.57 CBV.dial AIV @14:32:05.03 PRV.dial between CBV and air @14:32:05.35 PRV.dial :air @14:32:05.67 PS.reconfigure PSslow @14:32:05.71 PS.seek 0.599ml @14:32:08.25 PRV.dial :waste2 @14:32:08.98 PS.reconfigure PSconfig @14:32:09.01 PS.seek empty @14:32:15.91 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:32:16.05 Elbow.seek FlushPuck::Garage @14:32:17.75 Elbow.reconfigure LooseElbow @14:32:18.40 Forearm.seek garage @14:32:19.45 Hand.grip @14:32:20.22 Forearm.seek retract @14:32:21.25 Elbow.reconfigure TightElbow @14:32:21.29 Elbow.seek CC @14:32:24.06 Elbow.reconfigure LooseElbow @14:32:24.14 Forearm.reconfigure TightForearm @14:32:24.17 Forearm.seek CC @14:32:25.22 CC.seek closed @14:32:29.01 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 2.06 @14:32:29.06 Hand.release @14:32:29.80 Forearm.reconfigure LooseForearm @14:32:29.83 Forearm.seek retract @14:32:30.83 Clearing PML for modified lysate @14:32:30.84 CTV.dial :air,avoiding => intake PRV.dial :waste2 @14:32:30.85 PTV.dial :PRV,avoiding => puck @14:32:31.59 delay 2 seconds @14:32:33.59 PTV.dial :PRVmixing,avoiding => puck @14:32:34.31 delay 2 seconds @14:32:36.32 PSV.dial :PML @14:32:36.33 delay 2 seconds @14:32:38.33 PRV.dial :air @14:32:39.06 delay 2 seconds @14:32:41.12 PS.seek 1.499ml @14:32:57.19 delay 5 seconds @14:33:02.20 PS.seek 2.500ml @14:33:13.22 PSV.dial :air @14:33:13.68 PS.seek 0.000ml @14:33:39.87 mixing lysate with speDil @14:33:39.88 PSV.dial :PML @14:33:40.44 CBV.dial :PRV @14:33:40.45 PRV.dial :air @14:33:40.46 delay 2 seconds @14:33:42.47 PRV.dial :speDil @14:33:42.90 delay 2 seconds @14:33:44.96 PS.reconfigure PSslow1 @14:33:44.99 PS.seek 0.225ml @14:34:08.34 delay 5 seconds @14:34:13.35 PRV.dial CBV @14:34:14.09 delay 2 seconds @14:34:16.15 PS.seek 0.475ml @14:34:41.90 delay 5 seconds @14:34:46.91 PRV.dial :air @14:34:47.35 delay 2 seconds @14:34:49.36 PS.seek 0.925ml @14:35:35.37 delay 10 seconds @14:35:45.38 PRV.dial CBV @14:35:45.84 CTV.dial :air,avoiding => intake @14:35:45.85 PSV.dial :air @14:35:46.28 PS.reconfigure PSconfig @14:35:46.31 PS.seek 2.500ml @14:36:03.12 delay 3 seconds @14:36:06.13 PSV.dial PTV @14:36:06.98 PTV.dial :PRV,avoiding => puck @14:36:07.72 PS.seek 0.000ml @14:36:33.96 PSV.dial :air @14:36:34.67 PS.seek 2.500ml @14:37:00.83 delay 3 seconds @14:37:03.84 PSV.dial PTV @14:37:04.57 PTV.dial :PRV,avoiding => puck @14:37:04.59 PS.seek 0.000ml @14:37:30.83 Pushing modified lysate into AIV Injection Loop @14:37:30.84 CBV.dial AIV @14:37:30.85 AIV.dial :bypass @14:37:30.86 PRV.dial CBV @14:37:30.87 PSV.dial :air @14:37:31.61 PS.seek 2.500ml @14:37:57.93 delay 3 seconds @14:38:00.95 PSV.dial :PML @14:38:01.38 PTV.dial :PRVmixing,avoiding => puck @14:38:02.15 PS.reconfigure PSslow1 @14:38:02.18 PS.seek 1.850ml @14:39:08.58 delay 5 seconds @14:39:13.59 AIV.dial :sample @14:39:14.12 AWV.select :waste @14:39:14.28 PS.reconfigure PSslow2 @14:39:14.31 PS.seek 1.310ml @14:41:04.36 delay 15 seconds @14:41:19.37 AIV.dial :bypass @14:41:19.90 PS.reconfigure PSconfig @14:41:19.93 PS.seek 0.000ml @14:41:34.04 PSV.dial :air @14:41:34.49 PS.seek 2.500ml @14:42:00.73 PSV.dial :PML @14:42:01.18 PS.seek 0.000ml @14:42:27.48 AIV.dial :coilToMFB @14:42:27.49 AWV.select :air @14:42:27.63 Extracting Nucleic Acid from AIV @14:42:28.18
Beginning samplePassD @14:42:28.19 SPE.hold 2:46:40,55.0C @14:42:28.21 ASV.dial :SPEdelivery @14:42:28.34
AWV.select :air @14:42:28.35 AIV.dial :bypass PSV.dial PSR @14:42:28.93 delay 20 seconds @14:42:29.38
PSR.select :killMFB @14:42:29.48 PS.seek 1.500ml @14:42:45.60 delay 5 seconds @14:42:48.94 primeLysateINT @14:42:48.95 AWV.select :air @14:42:48.96 AIV.dial :coilToMFB @14:42:48.97 ATV.dial :AIV @14:42:50.30 AS.reconfigure ASslow1 @14:42:50.33 AS.seek 0.239ml @14:42:50.61
PSV.dial :waste1 @14:42:51.06 PS.seek 0.000ml @14:43:07.19 PSV.dial PSR @14:43:07.65 PSR.select :air @14:43:07.75 PS.seek 2.500ml @14:43:34.07 PRV.dial CBV @14:43:34.09
CBV.dial PRV @14:43:34.10 PSV.dial :mixloop @14:43:35.13 CTV.dial :air,avoiding => intake @14:43:35.14 PTV.dial :PRVmixing @14:43:35.16 PS.seek 0.000ml @14:43:39.57 delay 10 seconds @14:43:49.58 ATV.dial :air2 @14:43:50.17 AS.seek 0.269ml @14:43:56.52 ATV.dial :waste2 @14:43:56.98 AS.reconfigure ASnorm @14:43:57.01 AS.seek empty @14:44:01.41
PSV.dial PSR @14:44:02.46 PSR.select :flush @14:44:02.56 PS.seek 1.500ml @14:44:11.19 Lysate primed! @14:44:11.20 AFV.select :holdingCoil @14:44:11.21 ATV.dial :air2 @14:44:11.70 AS.reconfigure ASslow1 @14:44:11.73 AS.seek 0.069ml @14:44:18.63
delay 5 seconds @14:44:23.64 PSV.dial :waste1 @14:44:24.09 PS.seek 0.000ml @14:44:26.30 delay 3 seconds @14:44:29.31 ATV.dial :AIV @14:44:29.87 AS.seek 0.469ml @14:44:40.10
PSV.dial PSR @14:44:40.55 PSR.select :air @14:44:40.65 PS.seek 2.500ml @14:45:06.91 PRV.dial CBV @14:45:06.93
CBV.dial PRV @14:45:06.94 PSV.dial :mixloop @14:45:07.95 CTV.dial :air,avoiding => intake @14:45:07.96 PTV.dial :PRVmixing @14:45:07.98 PS.seek 0.000ml @14:45:34.19 PSV.dial PSR @14:45:35.20 PSR.select :flush @14:45:35.30 PS.seek 1.500ml @14:45:51.34 delay 5 seconds @14:45:51.67 delay 5 seconds @14:45:56.35
PSV.dial :waste1 @14:45:56.72 Solid Phase Extraction Heater at 52.8C @14:45:56.73 ATV.dial ASV @14:45:56.79
PS.seek 0.000ml @14:45:57.06 ASV.dial :SPEdelivery @14:45:57.12 AS.seek 0.041ml @14:46:12.91
PSV.dial PSR @14:46:13.47 PSR.select :air @14:46:13.57 PS.seek 2.500ml @14:46:39.84 PRV.dial CBV @14:46:39.86
CBV.dial PRV @14:46:39.87 PSV.dial :mixloop @14:46:40.89 CTV.dial :air,avoiding => intake @14:46:40.90 PTV.dial :PRVmixing @14:46:40.92 PS.seek 0.000ml @14:47:07.19 PSV.dial PSR @14:47:08.32 PSR.select :flush @14:47:08.42 PS.seek 1.500ml @14:47:24.54 delay 5 seconds @14:47:24.66 delay 10 seconds @14:47:29.55
PSV.dial :waste1 @14:47:30.02 PS.seek 0.000ml @14:47:34.67 rinseLysateLineINT @14:47:34.68 AFV.select :carrierSolution @14:47:34.77 delay 2 seconds @14:47:36.78 AS.reconfigure ASconfig @14:47:36.81 AS.seek 1.000ml @14:47:46.22
PSV.dial PSR @14:47:46.68 PSR.select :air @14:47:46.78 PS.seek 2.500ml @14:48:12.97 PRV.dial CBV @14:48:12.99
CBV.dial PRV @14:48:13.00 PSV.dial :mixloop @14:48:14.00 CTV.dial :air,avoiding => intake @14:48:14.01 PTV.dial :PRVmixing @14:48:14.02 PS.reconfigure PSslow @14:48:14.06 PS.seek 1.200ml @14:48:32.99 delay 1 second @14:48:34.00 AFV.select :holdingCoil @14:48:34.09 delay 1 second @14:48:35.10 ATV.dial :AIV @14:48:35.54 AIV.dial :coilToMFB @14:48:35.55 AWV.select :waste @14:48:35.64 AS.reconfigure ASslow0 @14:48:35.68 AS.seek 0.000ml @14:48:41.04
PSV.dial :waste1 @14:48:42.36 PS.reconfigure PSconfig @14:48:42.39 PS.seek 0.000ml @14:48:55.44 PSV.dial :air @14:48:56.47 PS.seek 2.500ml @14:49:22.75 PSV.dial :mixloop @14:49:23.20 PS.reconfigure PSslow @14:49:23.23 PS.seek 0.000ml @14:50:14.97 PSV.dial :air @14:50:15.57 PS.reconfigure PSconfig @14:50:15.60 PS.seek 2.500ml @14:50:17.99 ATV.dial :air2 @14:50:18.66 AS.reconfigure ASslow1 @14:50:18.70 AS.seek 0.050ml @14:50:29.17 ATV.dial :AIV @14:50:29.74 AS.seek 0.010ml @14:50:38.16 ATV.dial :waste @14:50:39.45 AS.seek empty @14:50:41.87
PSV.dial :mixloop @14:50:42.02 ethanol wash @14:50:42.03 ATV.dial :air2 @14:50:42.32
PS.reconfigure PSslow @14:50:42.35 PS.seek 0.000ml @14:50:42.97 AS.reconfigure ASslow2 @14:50:43.00 AS.seek 0.009ml @14:50:53.55 delay 3 seconds @14:50:56.56 Pulling 0.4ml of Ethanol Wash @14:50:56.57 ATV.dial :ethanol @14:50:57.22 AS.reconfigure ASslow1 @14:50:57.25 AS.seek 0.409ml @14:51:33.75
Cleaning Collection Syringe @14:51:33.76 CSV.dial CSR @14:51:34.19 CSR.select :flush @14:51:34.29 CS.seek 4.000ml @14:51:45.28 delay 5 seconds @14:51:50.29 CSR.select :air @14:51:50.38 CS.seek 10.000ml @14:52:06.34 CSV.dial CTV @14:52:06.36
CTV.dial CSV @14:52:07.66 CBV.dial :waste1 @14:52:08.15 CS.seek 0.000ml @14:52:19.12 delay 3 seconds @14:52:22.13 ATV.dial :air1 @14:52:22.45 delay 3 seconds @14:52:25.51 AS.seek 0.434ml @14:52:30.84 delay 3 seconds @14:52:33.85 ATV.dial :ethanol @14:52:34.22
CSV.dial CSR @14:52:34.31 AS.seek 0.419ml @14:52:34.72
CSR.select :flush @14:52:34.82 CS.seek 4.000ml @14:52:37.61 delay 3 seconds @14:52:40.62 ATV.dial ASV @14:52:41.75 AS.seek 0.092ml @14:52:45.78
delay 5 seconds @14:52:50.79 CSR.select :air @14:52:50.88 CS.seek 10.000ml @14:53:06.81 CSV.dial CTV @14:53:06.83
CTV.dial CSV @14:53:07.34 CBV.dial :waste1 @14:53:07.36 CS.seek 0.000ml @14:53:33.51 Garaging FlushPuck in Collection Clamp @14:53:33.56 Elbow.reconfigure TightElbow @14:53:33.59 Elbow.seek CC @14:53:33.68 Elbow.reconfigure LooseElbow @14:53:33.77 Forearm.seek CC @14:53:34.72 CC.seek sealed @14:53:35.65 Hand.grip @14:53:36.33 CC.seek open @14:53:39.62 Forearm.seek retract @14:53:40.57 Elbow.reconfigure TightElbow @14:53:40.61 Elbow.seek garage @14:53:43.33 Elbow.reconfigure LooseElbow @14:53:43.41 Forearm.reconfigure TightForearm @14:53:43.44 Forearm.seek garage @14:53:44.59 Hand.release @14:53:45.37 Forearm.reconfigure LooseForearm @14:53:45.40 Forearm.seek retract @14:53:46.52 Whole Cell Archive sampling 1000ml @14:53:46.53 Loading wcr puck from tube 3 into Collection Clamp @14:53:46.70 SC.reconfigure SCtight @14:53:46.73 SC.seek 3 @14:53:48.62 delay 3 seconds @14:53:48.97 SC.reconfigure SCloose @14:53:49.14 Elbow.reconfigure TightElbow @14:53:49.18 Elbow.seek 3 @14:53:50.97 Forearm.reconfigure TightForearm @14:53:51.00 Forearm.seek 3 + 800 counts @14:53:51.63 ATV.dial :waste2 @14:53:52.06 SE.reconfigure SEpick @14:53:52.11 SE.seek beamBreak @14:53:52.16 AS.reconfigure ASconfig @14:53:52.20 AS.seek empty @14:53:54.01 SE.configure SEconfig @14:53:54.13 Elbow.seek 3 @14:53:54.83 Forearm.seek 3 + 300 counts @14:53:55.06 Forearm.reconfigure LooseForearm @14:53:55.10 Hand.grip @14:53:55.85 SE.seek down @14:53:55.97 Forearm.seek retract @14:53:56.97 Elbow.seek CC @14:53:57.64 SE.coast @14:53:57.90 ATV.dial :air2 @14:53:58.26 AS.reconfigure ASslow1 @14:53:58.29 AS.seek 1.000ml @14:53:58.50 Elbow.reconfigure LooseElbow @14:53:58.58 Forearm.reconfigure TightForearm @14:53:58.61 Forearm.seek CC @14:53:59.72 CC.seek closed @14:54:03.48 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.95 @14:54:03.49 Hand.release @14:54:04.25 Forearm.reconfigure LooseForearm @14:54:04.28 Forearm.seek retract @14:54:05.27 SC.coast @14:54:05.30 collectSample 1000ml w/bubble point of 55psi @14:54:05.31 Priming 7ml @14:54:05.32 Sleepy.power :sampler,:ON Intake.select :close @14:54:05.33 Exhaust.select :close @14:54:05.34 SS.hold @14:54:05.50 Calibrating pressure sensors @14:54:05.51 CTV.dial :air @14:54:06.85 SSV.dial :spit,avoiding => exhaust @14:54:06.86 SS.reconfigure SSfast @14:54:06.89 SS.seek 5.00ml @14:54:09.55 SSV.dial CBV @14:54:09.57 CBV.dial SSV @14:54:10.97 delay 3.8 seconds @14:54:14.86 SSV.dial :spit @14:54:16.22 SS.seek 0.00ml @14:54:19.03 SSV.dial CBV @14:54:20.38 CTV.dial :intake @14:54:21.13 Waiting for external sampler to finish priming... @14:54:21.14 delayUntil "15:04:05.32" @14:57:22.64 delay 5 seconds @14:57:27.65 ATV.dial ASV @14:57:28.36 ASV.dial :SPEdelivery @14:57:28.38 AS.seek 0.020ml @15:00:48.54 delay 5 seconds @15:00:53.55 ATV.dial :air2 @15:00:54.41 AS.seek 0.520ml @15:02:36.67 delay 5 seconds @15:02:41.68 ATV.dial ASV @15:02:42.38 ASV.dial :wasteR @15:02:42.86 AS.seek 0.300ml @15:03:27.98 delay 20 seconds @15:03:48.00 AS.seek 0.070ml @15:04:05.33 SSV.dial CBV @15:04:05.35 CBV.dial SSV @15:04:05.36 Allowing 55s for 7.0ml of remaining 7.0ml @15:04:05.37 Intake.select :open @15:04:07.50 Intake @49.0psia, vacuum from 20.0 to 24.0psi @15:04:07.52 SS.reconfigure forSampling @15:04:07.55 SS.seek 7.00ml @15:04:14.04 Stroke completed -- waiting for pressure to equalize ... @15:04:14.68 Intake.select :close @15:04:14.82 SSV.dial :exhaust @15:04:15.62 Exhaust.select :open @15:04:15.72 SS.reconfigure SSexhale @15:04:15.76 SS.seek 0.00ml @15:04:19.34 Exhaust.select :close @15:04:19.45 Sampling 1000.0ml @15:04:19.47 Intake.select :close Exhaust.select :close @15:04:19.48 SS.reconfigure SSconfig @15:04:19.51 SS.hold @15:04:19.67 CTV.dial :intake @15:04:19.69 SSV.dial CBV @15:04:19.71 CBV.dial SSV @15:04:20.51 Allowing 185s for 25.0ml of remaining 1000.0ml @15:04:20.52 Intake.select :open @15:04:22.65 Intake @48.7psia, vacuum from 20.0 to 24.0psi @15:04:22.67 SS.reconfigure forSampling @15:04:22.70 SS.seek 25.00ml @15:04:35.16 delay 20 seconds @15:04:37.46 Stroke completed -- waiting for pressure to equalize ... @15:04:38.10 Intake.select :close @15:04:38.24 SSV.dial :exhaust @15:04:39.04 Exhaust.select :open @15:04:39.14 SS.reconfigure SSexhale @15:04:39.18 SS.seek 0.00ml @15:04:48.10 Exhaust.select :close @15:04:48.20 SSV.dial CBV @15:04:48.22 CBV.dial SSV @15:04:49.61 Allowing 185s for 25.0ml of remaining 975.0ml @15:04:49.62 Intake.select :open @15:04:51.75 Intake @47.8psia, vacuum from 20.0 to 24.0psi @15:04:51.77 SS.reconfigure forSampling @15:04:51.80 SS.seek 25.00ml @15:04:55.17 ATV.dial :waste2 @15:04:55.71 AS.reconfigure ASconfig @15:04:55.74 AS.seek empty @15:05:00.17 Elution @15:05:00.18 ATV.dial :air1 @15:05:01.16 AS.reconfigure ASslow1 @15:05:01.19 AS.seek 0.049ml @15:05:07.15 Stroke completed -- waiting for pressure to equalize ... @15:05:07.79 Intake.select :close @15:05:07.93 SSV.dial :exhaust @15:05:08.71 Exhaust.select :open @15:05:08.81 SS.reconfigure SSexhale @15:05:08.85 SS.seek 0.00ml @15:05:11.69 delay 3 seconds @15:05:14.70 ATV.dial :ElutionWater @15:05:15.28 AS.seek 0.109ml @15:05:17.52 Exhaust.select :close @15:05:17.62 SSV.dial CBV @15:05:17.64 CBV.dial SSV @15:05:18.45 Allowing 185s for 25.0ml of remaining 950.0ml @15:05:18.47 Intake.select :open @15:05:20.60 Intake @48.9psia, vacuum from 20.0 to 24.0psi @15:05:20.62 SS.reconfigure forSampling @15:05:20.65 SS.seek 25.00ml @15:05:27.76 delay 3 seconds @15:05:30.77 ATV.dial ASV,via => air2 @15:05:32.48 ASV.dial :SPEelution @15:05:33.15 AFV.select :carrierSolution @15:05:33.30 AS.reconfigure ASnorm @15:05:33.33 AS.seek 0.759ml @15:05:35.91 Stroke completed -- waiting for pressure to equalize ... @15:05:36.55 Intake.select :close @15:05:36.69 SSV.dial :exhaust @15:05:37.48 Exhaust.select :open @15:05:37.58 SS.reconfigure SSexhale @15:05:37.62 SS.seek 0.00ml @15:05:46.34 Exhaust.select :close @15:05:46.44 SSV.dial CBV @15:05:46.46 CBV.dial SSV @15:05:47.26 Allowing 185s for 25.0ml of remaining 925.0ml @15:05:47.27 Intake.select :open @15:05:49.41 Intake @47.7psia, vacuum from 20.0 to 24.0psi @15:05:49.43 SS.reconfigure forSampling @15:05:49.46 SS.seek 25.00ml @15:06:04.38 Stroke completed -- waiting for pressure to equalize ... @15:06:05.02 Intake.select :close @15:06:05.17 SSV.dial :exhaust @15:06:05.98 Exhaust.select :open @15:06:06.08 SS.reconfigure SSexhale @15:06:06.12 SS.seek 0.00ml @15:06:06.84 delay 3 seconds @15:06:09.85 AFV.select :holdingCoil @15:06:09.99 AS.reconfigure ASslow1 @15:06:10.02 AS.seek 0.489ml @15:06:15.01 Exhaust.select :close @15:06:15.12 SSV.dial CBV @15:06:15.14 CBV.dial SSV @15:06:15.96 Allowing 185s for 25.0ml of remaining 900.0ml @15:06:15.98 Intake.select :open @15:06:18.11 Intake @47.1psia, vacuum from 20.0 to 24.0psi @15:06:18.13 SS.reconfigure forSampling @15:06:18.16 SS.seek 25.00ml @15:06:28.19 Vacuum too high -- waiting up to 40s @15:06:28.46 Intake @40.2psia, vacuum from 20.0 to 24.0psi @15:06:28.47 SS.reconfigure forSampling @15:06:28.51 SS.seek 25.00ml @15:06:33.24 Stroke completed -- waiting for pressure to equalize ... @15:06:33.88 Intake.select :close @15:06:34.01 SSV.dial :exhaust @15:06:34.82 Exhaust.select :open @15:06:34.92 SS.reconfigure SSexhale @15:06:34.96 SS.seek 0.00ml @15:06:43.94 Exhaust.select :close @15:06:44.04 SSV.dial CBV @15:06:44.06 CBV.dial SSV @15:06:44.86 Allowing 185s for 25.0ml of remaining 875.0ml @15:06:44.87 Intake.select :open @15:06:47.01 Intake @47.2psia, vacuum from 20.0 to 24.0psi @15:06:47.03 SS.reconfigure forSampling @15:06:47.06 SS.seek 25.00ml @15:06:59.78 Vacuum too high -- waiting up to 40s @15:07:00.05 Intake @41.1psia, vacuum from 20.0 to 24.0psi @15:07:00.06 SS.reconfigure forSampling @15:07:00.10 SS.seek 25.00ml @15:07:03.39 Stroke completed -- waiting for pressure to equalize ... @15:07:04.03 Intake.select :close @15:07:04.18 SSV.dial :exhaust @15:07:04.97 Exhaust.select :open @15:07:05.07 SS.reconfigure SSexhale @15:07:05.11 SS.seek 0.00ml @15:07:05.34 delay 5 seconds @15:07:10.40 AS.reconfigure ASslow2 @15:07:10.43 AS.seek 0.454ml @15:07:13.92 Exhaust.select :close @15:07:14.02 SSV.dial CBV @15:07:14.04 CBV.dial SSV @15:07:14.85 Allowing 185s for 25.0ml of remaining 850.0ml @15:07:14.86 Intake.select :open @15:07:17.00 Intake @47.4psia, vacuum from 20.0 to 24.0psi @15:07:17.51 SS.reconfigure forSampling @15:07:17.54 SS.seek 25.00ml @15:07:33.29 Vacuum too high -- waiting up to 40s @15:07:33.55 Intake @42.6psia, vacuum from 20.0 to 24.0psi @15:07:33.56 SS.reconfigure forSampling @15:07:33.60 SS.seek 25.00ml @15:07:35.52 Stroke completed -- waiting for pressure to equalize ... @15:07:36.16 Intake.select :close @15:07:36.30 SSV.dial :exhaust @15:07:37.11 Exhaust.select :open @15:07:37.21 SS.reconfigure SSexhale @15:07:37.25 SS.seek 0.00ml @15:07:46.03 Exhaust.select :close @15:07:46.13 SSV.dial CBV @15:07:46.15 CBV.dial SSV @15:07:46.43 delay 1:00 @15:07:46.97 Allowing 185s for 25.0ml of remaining 825.0ml @15:07:46.98 Intake.select :open @15:07:49.12 Intake @47.2psia, vacuum from 20.0 to 24.0psi @15:07:49.14 SS.reconfigure forSampling @15:07:49.17 SS.seek 25.00ml @15:08:04.22 Vacuum too high -- waiting up to 40s @15:08:04.49 Intake @43.3psia, vacuum from 20.0 to 24.0psi @15:08:04.51 SS.reconfigure forSampling @15:08:04.54 SS.seek 25.00ml @15:08:09.25 Stroke completed -- waiting for pressure to equalize ... @15:08:09.89 Intake.select :close @15:08:10.02 SSV.dial :exhaust @15:08:10.83 Exhaust.select :open @15:08:10.93 SS.reconfigure SSexhale @15:08:10.97 SS.seek 0.00ml @15:08:20.03 Exhaust.select :close @15:08:20.13 SSV.dial CBV @15:08:20.15 CBV.dial SSV @15:08:20.95 Allowing 185s for 25.0ml of remaining 800.0ml @15:08:20.96 Intake.select :open @15:08:23.10 Intake @47.0psia, vacuum from 20.0 to 24.0psi @15:08:23.12 SS.reconfigure forSampling @15:08:23.15 SS.seek 25.00ml @15:08:38.79 Vacuum too high -- waiting up to 40s @15:08:39.06 Intake @43.6psia, vacuum from 20.0 to 24.0psi @15:08:39.08 SS.reconfigure forSampling @15:08:39.11 SS.seek 25.00ml @15:08:46.26 Stroke completed -- waiting for pressure to equalize ... @15:08:46.44 pushing eluate to holding coil @15:08:46.50 AS.seek 0.294ml @15:08:46.90 Intake.select :close @15:08:47.04 SSV.dial :exhaust @15:08:47.84 Exhaust.select :open @15:08:47.94 SS.reconfigure SSexhale @15:08:47.98 SS.seek 0.00ml @15:08:56.65 Exhaust.select :close @15:08:56.75 SSV.dial CBV @15:08:56.77 CBV.dial SSV @15:08:57.59 Allowing 185s for 25.0ml of remaining 775.0ml @15:08:57.60 Intake.select :open @15:08:59.74 Intake @47.9psia, vacuum from 20.0 to 24.0psi @15:08:59.76 SS.reconfigure forSampling @15:08:59.79 SS.seek 25.00ml @15:09:15.78 Vacuum too high -- waiting up to 40s @15:09:16.04 Intake @45.2psia, vacuum from 20.0 to 24.0psi @15:09:16.05 SS.reconfigure forSampling @15:09:16.09 SS.seek 25.00ml @15:09:27.70 Stroke completed -- waiting for pressure to equalize ... @15:09:28.34 Intake.select :close @15:09:28.47 SSV.dial :exhaust @15:09:29.28 Exhaust.select :open @15:09:29.38 SS.reconfigure SSexhale @15:09:29.42 SS.seek 0.00ml @15:09:38.23 Exhaust.select :close @15:09:38.33 SSV.dial CBV @15:09:38.35 CBV.dial SSV @15:09:39.17 Allowing 185s for 25.0ml of remaining 750.0ml @15:09:39.18 Intake.select :open @15:09:41.32 Intake @48.2psia, vacuum from 20.0 to 24.0psi @15:09:41.34 SS.reconfigure forSampling @15:09:41.37 SS.seek 25.00ml @15:09:57.03 Vacuum too high -- waiting up to 40s @15:09:57.29 Intake @45.5psia, vacuum from 20.0 to 24.0psi @15:09:57.30 SS.reconfigure forSampling @15:09:57.34 SS.seek 25.00ml @15:10:16.74 Stroke completed -- waiting for pressure to equalize ... @15:10:17.38 Intake.select :close @15:10:17.51 SSV.dial :exhaust @15:10:18.31 Exhaust.select :open @15:10:18.41 SS.reconfigure SSexhale @15:10:18.45 SS.seek 0.00ml @15:10:27.49 Exhaust.select :close @15:10:27.59 SSV.dial CBV @15:10:27.61 CBV.dial SSV @15:10:28.43 Allowing 185s for 25.0ml of remaining 725.0ml @15:10:28.44 Intake.select :open @15:10:30.58 Intake @47.7psia, vacuum from 20.0 to 24.0psi @15:10:30.60 SS.reconfigure forSampling @15:10:30.63 SS.seek 25.00ml @15:11:01.78 Vacuum too high -- waiting up to 40s @15:11:02.04 Intake @46.9psia, vacuum from 20.0 to 24.0psi @15:11:02.05 SS.reconfigure forSampling @15:11:02.08 SS.seek 25.00ml @15:11:15.90 Stroke completed -- waiting for pressure to equalize ... @15:11:17.13 Intake.select :close @15:11:17.27 SSV.dial :exhaust @15:11:18.07 Exhaust.select :open @15:11:18.17 SS.reconfigure SSexhale @15:11:18.21 SS.seek 0.00ml @15:11:26.97 Exhaust.select :close @15:11:27.07 SSV.dial CBV @15:11:27.09 CBV.dial SSV @15:11:27.90 Allowing 185s for 25.0ml of remaining 700.0ml @15:11:27.91 Intake.select :open @15:11:30.05 Intake @47.7psia, vacuum from 20.0 to 24.0psi @15:11:30.07 SS.reconfigure forSampling @15:11:30.10 SS.seek 25.00ml @15:11:30.15 delay 30 seconds @15:12:00.16 ATV.dial :waste,via => air3 @15:12:01.67 AS.reconfigure ASconfig @15:12:01.70 AS.seek empty @15:12:19.20 Parking Bleach in LysateSource line and SPEcolumn @15:12:19.21 ATV.dial :air3 @15:12:19.89 AS.reconfigure ASslow1 @15:12:19.92 AS.seek 0.029ml @15:12:26.26 ATV.dial :ElutionWater @15:12:26.66 AS.seek 0.059ml @15:12:29.25 Stroke completed -- waiting for pressure to equalize ... @15:12:30.48 Intake.select :close @15:12:30.62 SSV.dial :exhaust @15:12:31.42 Exhaust.select :open @15:12:31.52 SS.reconfigure SSexhale @15:12:31.56 SS.seek 0.00ml @15:12:33.05 ATV.dial :air3 @15:12:33.46 AS.seek 0.069ml @15:12:35.72 delay 2 seconds @15:12:37.73 ATV.dial :bleach @15:12:38.13 AS.seek 0.709ml @15:12:40.74 Exhaust.select :close @15:12:40.84 SSV.dial CBV @15:12:40.86 CBV.dial SSV @15:12:41.67 Allowing 185s for 25.0ml of remaining 675.0ml @15:12:41.68 Intake.select :open @15:12:43.82 Intake @48.2psia, vacuum from 20.0 to 24.0psi @15:12:43.84 SS.reconfigure forSampling @15:12:43.87 SS.seek 25.00ml @15:13:16.17 Vacuum too high -- waiting up to 40s @15:13:16.44 Intake @47.1psia, vacuum from 20.0 to 24.0psi @15:13:16.45 SS.reconfigure forSampling @15:13:16.49 SS.seek 25.00ml @15:14:06.95 Stroke completed -- waiting for pressure to equalize ... @15:14:08.17 Intake.select :close @15:14:08.30 SSV.dial :exhaust @15:14:09.11 Exhaust.select :open @15:14:09.21 SS.reconfigure SSexhale @15:14:09.25 SS.seek 0.00ml @15:14:18.04 Exhaust.select :close @15:14:18.14 SSV.dial CBV @15:14:18.16 CBV.dial SSV @15:14:18.98 Allowing 185s for 25.0ml of remaining 650.0ml @15:14:18.99 Intake.select :open @15:14:21.13 Intake @47.8psia, vacuum from 20.0 to 24.0psi @15:14:21.15 SS.reconfigure forSampling @15:14:21.18 SS.seek 25.00ml @15:14:48.90 delay 3 seconds @15:14:51.91 ATV.dial :air3 @15:14:52.24 delay 3 seconds @15:14:55.30 AS.seek 0.734ml @15:15:00.65 delay 3 seconds @15:15:03.66 ATV.dial :bleach @15:15:04.06 AS.seek 0.719ml @15:15:07.35 delay 3 seconds @15:15:10.36 ATV.dial :AIV @15:15:11.49 AS.reconfigure ASconfig @15:15:11.52 AS.seek 0.015ml @15:15:52.84 ATV.dial :waste @15:15:54.25 AS.seek empty @15:15:55.41 ATV.dial :air3 @15:15:56.00 AS.reconfigure ASslow1 @15:15:56.03 AS.seek 0.009ml @15:15:58.38 delay 2 seconds @15:16:00.39 ATV.dial :bleach @15:16:00.76 AS.seek 0.579ml @15:16:11.41 Stroke completed -- waiting for pressure to equalize ... @15:16:13.21 Intake.select :close @15:16:13.34 SSV.dial :exhaust @15:16:14.15 Exhaust.select :open @15:16:14.25 SS.reconfigure SSexhale @15:16:14.29 SS.seek 0.00ml @15:16:23.15 Exhaust.select :close @15:16:23.25 SSV.dial CBV @15:16:23.27 CBV.dial SSV @15:16:24.07 Allowing 185s for 25.0ml of remaining 625.0ml @15:16:24.08 Intake.select :open @15:16:26.22 Intake @48.0psia, vacuum from 20.0 to 24.0psi @15:16:26.24 SS.reconfigure forSampling @15:16:26.27 SS.seek 25.00ml @15:17:57.27 delay 3 seconds @15:18:00.28 ATV.dial :air3 @15:18:00.75 delay 3 seconds @15:18:03.81 AS.seek 0.604ml @15:18:09.16 delay 3 seconds @15:18:12.17 ATV.dial :bleach @15:18:13.08 AS.seek 0.589ml @15:18:16.39 delay 3 seconds @15:18:16.51 Stroke completed -- waiting for pressure to equalize ... @15:18:18.92 Intake.select :close @15:18:19.06 SSV.dial :exhaust @15:18:19.40 ATV.dial :ASV @15:18:19.86 Exhaust.select :open @15:18:19.96 SS.reconfigure SSexhale @15:18:20.00 SS.seek 0.00ml @15:18:20.32 ASV.dial :wasteR @15:18:21.29 AS.seek 0.000ml @15:18:29.08 Exhaust.select :close @15:18:29.18 SSV.dial CBV @15:18:29.20 CBV.dial SSV @15:18:30.01 Allowing 185s for 25.0ml of remaining 600.0ml @15:18:30.03 Intake.select :open @15:18:32.16 Intake @48.5psia, vacuum from 20.0 to 24.0psi @15:18:32.18 SS.reconfigure forSampling @15:18:32.21 SS.seek 25.00ml @15:19:17.05 Vacuum too high -- waiting up to 40s @15:19:17.32 Intake @47.3psia, vacuum from 20.0 to 24.0psi @15:19:17.34 SS.reconfigure forSampling @15:19:17.37 SS.seek 25.00ml @15:19:43.87 Vacuum too high -- waiting up to 40s @15:19:44.36 Intake @47.4psia, vacuum from 20.0 to 24.0psi @15:19:44.37 SS.reconfigure forSampling @15:19:44.41 SS.seek 25.00ml @15:19:51.56 Vacuum too high -- waiting up to 40s @15:19:52.03 Intake @47.7psia, vacuum from 20.0 to 24.0psi @15:19:52.05 SS.reconfigure forSampling @15:19:52.08 SS.seek 25.00ml @15:19:55.75 Vacuum too high -- waiting up to 40s @15:19:56.23 SS.seek 25.00ml @15:19:59.84 Vacuum too high -- waiting up to 40s @15:20:00.32 SS.seek 25.00ml @15:20:04.40 Vacuum too high -- waiting up to 40s @15:20:04.89 Intake @48.2psia, vacuum from 20.0 to 24.0psi @15:20:04.90 SS.reconfigure forSampling @15:20:04.94 SS.seek 25.00ml @15:20:08.03 Vacuum too high -- waiting up to 40s @15:20:08.50 Intake @48.0psia, vacuum from 20.0 to 24.0psi @15:20:08.51 SS.reconfigure forSampling @15:20:08.55 SS.seek 25.00ml @15:20:11.83 Vacuum too high -- waiting up to 40s @15:20:12.31 Intake @47.7psia, vacuum from 20.0 to 24.0psi @15:20:12.32 SS.reconfigure forSampling @15:20:12.36 SS.seek 25.00ml @15:20:15.62 Vacuum too high -- waiting up to 40s @15:20:16.09 Intake @47.9psia, vacuum from 20.0 to 24.0psi @15:20:16.10 SS.reconfigure forSampling @15:20:16.14 SS.seek 25.00ml @15:20:19.65 Vacuum too high -- waiting up to 40s @15:20:20.15 Intake @48.0psia, vacuum from 20.0 to 24.0psi @15:20:20.17 SS.reconfigure forSampling @15:20:20.20 SS.seek 25.00ml @15:20:21.73 delay 1:00 @15:20:22.87 Vacuum too high -- waiting up to 40s @15:20:23.36 Intake @47.8psia, vacuum from 20.0 to 24.0psi @15:20:23.37 SS.reconfigure forSampling @15:20:23.41 SS.seek 25.00ml @15:20:26.06 Vacuum too high -- waiting up to 40s @15:20:26.55 Intake @47.9psia, vacuum from 20.0 to 24.0psi @15:20:26.56 SS.reconfigure forSampling @15:20:26.60 SS.seek 25.00ml @15:20:29.81 Vacuum too high -- waiting up to 40s @15:20:30.29 Intake @48.2psia, vacuum from 20.0 to 24.0psi @15:20:30.30 SS.reconfigure forSampling @15:20:30.34 SS.seek 25.00ml @15:20:30.93 Stroke completed -- waiting for pressure to equalize ... @15:20:33.33 Intake.select :close @15:20:33.46 SSV.dial :exhaust @15:20:34.80 Exhaust.select :open @15:20:34.90 SS.reconfigure SSexhale @15:20:34.94 SS.seek 0.00ml @15:20:43.77 Exhaust.select :close @15:20:43.87 SSV.dial CBV @15:20:43.89 CBV.dial SSV @15:20:44.71 Allowing 185s for 25.0ml of remaining 575.0ml @15:20:44.72 Intake.select :open @15:20:46.86 Intake @48.9psia, vacuum from 20.0 to 24.0psi @15:20:46.88 SS.reconfigure forSampling @15:20:46.91 SS.seek 25.00ml @15:20:49.98 Vacuum too high -- waiting up to 40s @15:20:50.46 Intake @48.2psia, vacuum from 20.0 to 24.0psi @15:20:50.48 SS.reconfigure forSampling @15:20:50.51 SS.seek 25.00ml @15:20:53.19 Vacuum too high -- waiting up to 40s @15:20:53.68 Intake @47.9psia, vacuum from 20.0 to 24.0psi @15:20:53.70 SS.reconfigure forSampling @15:20:53.73 SS.seek 25.00ml @15:20:56.60 Vacuum too high -- waiting up to 40s @15:20:57.09 Intake @48.1psia, vacuum from 20.0 to 24.0psi @15:20:57.11 SS.reconfigure forSampling @15:20:57.14 SS.seek 25.00ml @15:21:00.63 Vacuum too high -- waiting up to 40s @15:21:01.11 Intake @48.1psia, vacuum from 20.0 to 24.0psi @15:21:01.12 SS.reconfigure forSampling @15:21:01.16 SS.seek 25.00ml @15:21:03.85 Vacuum too high -- waiting up to 40s @15:21:04.34 Intake @48.0psia, vacuum from 20.0 to 24.0psi @15:21:04.36 SS.reconfigure forSampling @15:21:04.39 SS.seek 25.00ml @15:21:06.76 Vacuum too high -- waiting up to 40s @15:21:07.25 Intake @47.8psia, vacuum from 20.0 to 24.0psi @15:21:07.26 SS.reconfigure forSampling @15:21:07.30 SS.seek 25.00ml @15:21:10.16 Vacuum too high -- waiting up to 40s @15:21:10.65 Intake @48.3psia, vacuum from 20.0 to 24.0psi @15:21:10.66 SS.reconfigure forSampling @15:21:10.70 SS.seek 25.00ml @15:21:13.29 Vacuum too high -- waiting up to 40s @15:21:13.78 Intake @48.0psia, vacuum from 20.0 to 24.0psi @15:21:13.80 SS.reconfigure forSampling @15:21:13.83 SS.seek 25.00ml @15:21:16.16 Vacuum too high -- waiting up to 40s @15:21:16.65 Intake @47.8psia, vacuum from 20.0 to 24.0psi @15:21:16.66 SS.reconfigure forSampling @15:21:16.69 SS.seek 25.00ml @15:21:19.32 Vacuum too high -- waiting up to 40s @15:21:19.81 Intake @48.2psia, vacuum from 20.0 to 24.0psi @15:21:19.82 SS.reconfigure forSampling @15:21:19.86 SS.seek 25.00ml @15:21:21.75 AFV.select :carrierSolution @15:21:21.84 delay 2 seconds @15:21:22.35 Vacuum too high -- waiting up to 40s @15:21:22.84 Intake @48.4psia, vacuum from 20.0 to 24.0psi @15:21:22.86 SS.reconfigure forSampling @15:21:22.89 SS.seek 25.00ml @15:21:23.85 AS.reconfigure ASconfig @15:21:23.89 AS.seek 1.000ml @15:21:24.97 Vacuum too high -- waiting up to 40s @15:21:25.46 Intake @48.1psia, vacuum from 20.0 to 24.0psi @15:21:25.48 SS.reconfigure forSampling @15:21:25.51 SS.seek 25.00ml @15:21:27.69 Vacuum too high -- waiting up to 40s @15:21:28.18 Intake @48.0psia, vacuum from 20.0 to 24.0psi @15:21:28.20 SS.reconfigure forSampling @15:21:28.23 SS.seek 25.00ml @15:21:30.22 Vacuum too high -- waiting up to 40s @15:21:30.71 Intake @47.8psia, vacuum from 20.0 to 24.0psi @15:21:30.73 SS.reconfigure forSampling @15:21:30.76 SS.seek 25.00ml @15:21:33.00 Vacuum too high -- waiting up to 40s @15:21:33.49 Intake @48.0psia, vacuum from 20.0 to 24.0psi @15:21:33.51 SS.reconfigure forSampling @15:21:33.54 SS.seek 25.00ml @15:21:35.66 Vacuum too high -- waiting up to 40s @15:21:36.15 Intake @48.4psia, vacuum from 20.0 to 24.0psi @15:21:36.71 SS.reconfigure forSampling @15:21:36.74 SS.seek 25.00ml @15:21:39.13 Vacuum too high -- waiting up to 40s @15:21:39.62 Intake @48.0psia, vacuum from 20.0 to 24.0psi @15:21:39.64 SS.reconfigure forSampling @15:21:39.67 SS.seek 25.00ml @15:21:41.69 Vacuum too high -- waiting up to 40s @15:21:42.19 SS.seek 25.00ml @15:21:44.03 Vacuum too high -- waiting up to 40s @15:21:44.52 Intake @48.1psia, vacuum from 20.0 to 24.0psi @15:21:44.54 SS.reconfigure forSampling @15:21:44.57 SS.seek 25.00ml @15:21:46.60 Vacuum too high -- waiting up to 40s @15:21:47.09 Intake @48.2psia, vacuum from 20.0 to 24.0psi @15:21:47.11 SS.reconfigure forSampling @15:21:47.14 SS.seek 25.00ml @15:21:49.03 Vacuum too high -- waiting up to 40s @15:21:49.52 Intake @48.4psia, vacuum from 20.0 to 24.0psi @15:21:49.54 SS.reconfigure forSampling @15:21:49.57 SS.seek 25.00ml @15:21:51.32 Vacuum too high -- waiting up to 40s @15:21:51.81 Intake @48.1psia, vacuum from 20.0 to 24.0psi @15:21:51.83 SS.reconfigure forSampling @15:21:51.86 SS.seek 25.00ml @15:21:53.82 Vacuum too high -- waiting up to 40s @15:21:54.31 Intake @48.5psia, vacuum from 20.0 to 24.0psi @15:21:54.32 SS.reconfigure forSampling @15:21:54.36 SS.seek 25.00ml @15:21:56.07 Vacuum too high -- waiting up to 40s @15:21:56.56 Intake @48.0psia, vacuum from 20.0 to 24.0psi @15:21:56.57 SS.reconfigure forSampling @15:21:56.61 SS.seek 25.00ml @15:21:58.63 Vacuum too high -- waiting up to 40s @15:21:59.12 Intake @47.8psia, vacuum from 20.0 to 24.0psi @15:21:59.14 SS.reconfigure forSampling @15:21:59.17 SS.seek 25.00ml @15:22:01.06 Vacuum too high -- waiting up to 40s @15:22:01.55 Intake @47.9psia, vacuum from 20.0 to 24.0psi @15:22:01.57 SS.reconfigure forSampling @15:22:01.60 SS.seek 25.00ml @15:22:03.38 Vacuum too high -- waiting up to 40s @15:22:03.87 Intake @48.0psia, vacuum from 20.0 to 24.0psi @15:22:03.88 SS.reconfigure forSampling @15:22:03.92 SS.seek 25.00ml @15:22:05.60 Vacuum too high -- waiting up to 40s @15:22:06.08 Intake @48.5psia, vacuum from 20.0 to 24.0psi @15:22:06.09 SS.reconfigure forSampling @15:22:06.13 SS.seek 25.00ml @15:22:07.91 Vacuum too high -- waiting up to 40s @15:22:08.40 Intake @47.7psia, vacuum from 20.0 to 24.0psi @15:22:08.42 SS.reconfigure forSampling @15:22:08.45 SS.seek 25.00ml @15:22:10.25 Vacuum too high -- waiting up to 40s @15:22:10.74 Intake @47.9psia, vacuum from 20.0 to 24.0psi @15:22:10.75 SS.reconfigure forSampling @15:22:10.79 SS.seek 25.00ml @15:22:12.53 Vacuum too high -- waiting up to 40s @15:22:13.02 Intake @47.7psia, vacuum from 20.0 to 24.0psi @15:22:13.04 SS.reconfigure forSampling @15:22:13.07 SS.seek 25.00ml @15:22:14.88 Vacuum too high -- waiting up to 40s @15:22:15.36 Intake @47.9psia, vacuum from 20.0 to 24.0psi @15:22:15.38 SS.reconfigure forSampling @15:22:15.41 SS.seek 25.00ml @15:22:16.91 Vacuum too high -- waiting up to 40s @15:22:17.41 SS.seek 25.00ml @15:22:19.41 Vacuum too high -- waiting up to 40s @15:22:19.89 Intake @48.4psia, vacuum from 20.0 to 24.0psi @15:22:20.46 SS.reconfigure forSampling @15:22:20.49 SS.seek 25.00ml @15:22:22.51 delay 1 second @15:22:22.56 Vacuum too high -- waiting up to 40s @15:22:23.05 Intake @47.7psia, vacuum from 20.0 to 24.0psi @15:22:23.07 SS.reconfigure forSampling @15:22:23.10 SS.seek 25.00ml @15:22:23.52 AFV.select :holdingCoil @15:22:23.61 delay 1 second @15:22:24.62 ATV.dial :ASV @15:22:24.63 ASV.dial :wasteR @15:22:24.64 AS.reconfigure ASslow1 @15:22:24.67 AS.seek 0.000ml @15:22:24.88 Vacuum too high -- waiting up to 40s @15:22:25.38 Intake @47.8psia, vacuum from 20.0 to 24.0psi @15:22:25.39 SS.reconfigure forSampling @15:22:25.42 SS.seek 25.00ml @15:22:26.97 Vacuum too high -- waiting up to 40s @15:22:27.46 Intake @47.9psia, vacuum from 20.0 to 24.0psi @15:22:27.48 SS.reconfigure forSampling @15:22:27.51 SS.seek 25.00ml @15:22:29.38 Vacuum too high -- waiting up to 40s @15:22:29.87 Intake @48.0psia, vacuum from 20.0 to 24.0psi @15:22:29.89 SS.reconfigure forSampling @15:22:29.92 SS.seek 25.00ml @15:22:31.50 Vacuum too high -- waiting up to 40s @15:22:31.98 Intake @48.2psia, vacuum from 20.0 to 24.0psi @15:22:32.00 SS.reconfigure forSampling @15:22:32.03 SS.seek 25.00ml @15:22:33.53 Vacuum too high -- waiting up to 40s @15:22:34.01 Intake @47.8psia, vacuum from 20.0 to 24.0psi @15:22:34.03 SS.reconfigure forSampling @15:22:34.06 SS.seek 25.00ml @15:22:35.88 Vacuum too high -- waiting up to 40s @15:22:36.37 Intake @48.0psia, vacuum from 20.0 to 24.0psi @15:22:36.39 SS.reconfigure forSampling @15:22:36.42 SS.seek 25.00ml @15:22:38.03 Vacuum too high -- waiting up to 40s @15:22:38.51 Intake @48.4psia, vacuum from 20.0 to 24.0psi @15:22:38.53 SS.reconfigure forSampling @15:22:38.56 SS.seek 25.00ml @15:22:39.97 Vacuum too high -- waiting up to 40s @15:22:40.46 Intake @47.8psia, vacuum from 20.0 to 24.0psi @15:22:40.47 SS.reconfigure forSampling @15:22:40.51 SS.seek 25.00ml @15:22:42.06 Vacuum too high -- waiting up to 40s @15:22:42.54 Intake @47.8psia, vacuum from 20.0 to 24.0psi @15:22:42.56 SS.reconfigure forSampling @15:22:42.59 SS.seek 25.00ml @15:22:43.97 Vacuum too high -- waiting up to 40s @15:22:44.46 Intake @47.3psia, vacuum from 20.0 to 24.0psi @15:22:44.48 SS.reconfigure forSampling @15:22:44.51 SS.seek 25.00ml @15:22:46.28 Vacuum too high -- waiting up to 40s @15:22:46.98 Intake @47.1psia, vacuum from 20.0 to 24.0psi @15:22:47.00 SS.reconfigure forSampling @15:22:47.03 SS.seek 25.00ml @15:22:50.03 Vacuum too high -- waiting up to 40s @15:22:50.94 Intake @48.6psia, vacuum from 20.0 to 24.0psi @15:22:50.95 SS.reconfigure forSampling @15:22:50.99 SS.seek 25.00ml @15:22:52.41 Vacuum too high -- waiting up to 40s @15:22:52.89 Intake @47.6psia, vacuum from 20.0 to 24.0psi @15:22:52.91 SS.reconfigure forSampling @15:22:52.94 SS.seek 25.00ml @15:22:54.41 Vacuum too high -- waiting up to 40s @15:22:54.88 Intake @47.7psia, vacuum from 20.0 to 24.0psi @15:22:54.89 SS.reconfigure forSampling @15:22:54.92 SS.seek 25.00ml @15:22:56.62 Vacuum too high -- waiting up to 40s @15:22:57.10 Intake @47.9psia, vacuum from 20.0 to 24.0psi @15:22:57.11 SS.reconfigure forSampling @15:22:57.15 SS.seek 25.00ml @15:22:58.50 Vacuum too high -- waiting up to 40s @15:22:59.36 SS.seek 25.00ml @15:23:01.12 Vacuum too high -- waiting up to 40s @15:23:01.61 Intake @47.5psia, vacuum from 20.0 to 24.0psi @15:23:01.63 SS.reconfigure forSampling @15:23:01.66 SS.seek 25.00ml @15:23:03.28 Vacuum too high -- waiting up to 40s @15:23:03.77 Intake @47.3psia, vacuum from 20.0 to 24.0psi @15:23:03.79 SS.reconfigure forSampling @15:23:03.82 SS.seek 25.00ml @15:23:05.25 Vacuum too high -- waiting up to 40s @15:23:05.74 Intake @48.5psia, vacuum from 20.0 to 24.0psi @15:23:05.76 SS.reconfigure forSampling @15:23:05.79 SS.seek 25.00ml @15:23:07.06 Vacuum too high -- waiting up to 40s @15:23:07.55 Intake @48.2psia, vacuum from 20.0 to 24.0psi @15:23:07.57 SS.reconfigure forSampling @15:23:07.60 SS.seek 25.00ml @15:23:08.97 Vacuum too high -- waiting up to 40s @15:23:09.46 Intake @48.1psia, vacuum from 20.0 to 24.0psi @15:23:09.48 SS.reconfigure forSampling @15:23:09.51 SS.seek 25.00ml @15:23:10.78 Vacuum too high -- waiting up to 40s @15:23:11.27 Intake @47.9psia, vacuum from 20.0 to 24.0psi @15:23:11.28 SS.reconfigure forSampling @15:23:11.32 SS.seek 25.00ml @15:23:12.78 Vacuum too high -- waiting up to 40s @15:23:13.27 Intake @48.0psia, vacuum from 20.0 to 24.0psi @15:23:13.29 SS.reconfigure forSampling @15:23:13.32 SS.seek 25.00ml @15:23:14.59 Vacuum too high -- waiting up to 40s @15:23:15.08 Intake @48.0psia, vacuum from 20.0 to 24.0psi @15:23:15.10 SS.reconfigure forSampling @15:23:15.13 SS.seek 25.00ml @15:23:16.31 Vacuum too high -- waiting up to 40s @15:23:16.80 Intake @47.7psia, vacuum from 20.0 to 24.0psi @15:23:16.82 SS.reconfigure forSampling @15:23:16.85 SS.seek 25.00ml @15:23:18.44 Vacuum too high -- waiting up to 40s @15:23:19.14 Intake @47.9psia, vacuum from 20.0 to 24.0psi @15:23:19.16 SS.reconfigure forSampling @15:23:19.19 SS.seek 25.00ml @15:23:20.68 Stroke completed -- waiting for pressure to equalize ... @15:23:24.25 Intake.select :close @15:23:24.38 SSV.dial :exhaust @15:23:25.18 Exhaust.select :open @15:23:25.28 SS.reconfigure SSexhale @15:23:25.32 SS.seek 0.00ml @15:23:34.13 Exhaust.select :close @15:23:34.24 SSV.dial CBV @15:23:34.26 CBV.dial SSV @15:23:35.05 Allowing 185s for 25.0ml of remaining 550.0ml @15:23:35.06 Intake.select :open @15:23:37.20 Intake @48.3psia, vacuum from 20.0 to 24.0psi @15:23:37.22 SS.reconfigure forSampling @15:23:37.25 SS.seek 25.00ml @15:23:39.37 Vacuum too high -- waiting up to 40s @15:23:39.85 Intake @48.5psia, vacuum from 20.0 to 24.0psi @15:23:39.87 SS.reconfigure forSampling @15:23:39.90 SS.seek 25.00ml @15:23:41.15 Vacuum too high -- waiting up to 40s @15:23:41.63 Intake @48.5psia, vacuum from 20.0 to 24.0psi @15:23:41.65 SS.reconfigure forSampling @15:23:41.68 SS.seek 25.00ml @15:23:42.81 Vacuum too high -- waiting up to 40s @15:23:43.30 Intake @48.1psia, vacuum from 20.0 to 24.0psi @15:23:43.32 SS.reconfigure forSampling @15:23:43.35 SS.seek 25.00ml @15:23:44.81 Vacuum too high -- waiting up to 40s @15:23:45.30 Intake @48.4psia, vacuum from 20.0 to 24.0psi @15:23:45.32 SS.reconfigure forSampling @15:23:45.90 SS.seek 25.00ml @15:23:47.65 Vacuum too high -- waiting up to 40s @15:23:48.14 Intake @48.2psia, vacuum from 20.0 to 24.0psi @15:23:48.16 SS.reconfigure forSampling @15:23:48.19 SS.seek 25.00ml @15:23:49.53 Vacuum too high -- waiting up to 40s @15:23:50.02 Intake @48.0psia, vacuum from 20.0 to 24.0psi @15:23:50.04 SS.reconfigure forSampling @15:23:50.07 SS.seek 25.00ml @15:23:51.43 Vacuum too high -- waiting up to 40s @15:23:51.92 Intake @48.5psia, vacuum from 20.0 to 24.0psi @15:23:51.94 SS.reconfigure forSampling @15:23:51.97 SS.seek 25.00ml @15:23:53.34 Vacuum too high -- waiting up to 40s @15:23:53.83 Intake @48.8psia, vacuum from 20.0 to 24.0psi @15:23:53.85 SS.reconfigure forSampling @15:23:53.88 SS.seek 25.00ml @15:23:55.09 Vacuum too high -- waiting up to 40s @15:23:55.59 Intake @48.7psia, vacuum from 20.0 to 24.0psi @15:23:55.61 SS.reconfigure forSampling @15:23:55.64 SS.seek 25.00ml @15:23:57.00 Vacuum too high -- waiting up to 40s @15:23:57.49 Intake @48.6psia, vacuum from 20.0 to 24.0psi @15:23:57.51 SS.reconfigure forSampling @15:23:57.54 SS.seek 25.00ml @15:23:58.71 Vacuum too high -- waiting up to 40s @15:23:59.20 Intake @48.2psia, vacuum from 20.0 to 24.0psi @15:23:59.22 SS.reconfigure forSampling @15:23:59.25 SS.seek 25.00ml @15:24:00.56 Vacuum too high -- waiting up to 40s @15:24:01.05 Intake @47.8psia, vacuum from 20.0 to 24.0psi @15:24:01.06 SS.reconfigure forSampling @15:24:01.10 SS.seek 25.00ml @15:24:02.50 Vacuum too high -- waiting up to 40s @15:24:02.99 Intake @47.9psia, vacuum from 20.0 to 24.0psi @15:24:03.01 SS.reconfigure forSampling @15:24:03.04 SS.seek 25.00ml @15:24:04.34 Vacuum too high -- waiting up to 40s @15:24:04.83 Intake @47.7psia, vacuum from 20.0 to 24.0psi @15:24:04.84 SS.reconfigure forSampling @15:24:04.88 SS.seek 25.00ml @15:24:06.34 Vacuum too high -- waiting up to 40s @15:24:07.05 Intake @49.3psia, vacuum from 20.0 to 24.0psi @15:24:07.07 SS.reconfigure forSampling @15:24:07.10 SS.seek 25.00ml @15:24:08.43 Vacuum too high -- waiting up to 40s @15:24:08.92 Intake @48.0psia, vacuum from 20.0 to 24.0psi @15:24:08.93 SS.reconfigure forSampling @15:24:08.97 SS.seek 25.00ml @15:24:10.37 Vacuum too high -- waiting up to 40s @15:24:10.86 Intake @48.1psia, vacuum from 20.0 to 24.0psi @15:24:10.88 SS.reconfigure forSampling @15:24:10.91 SS.seek 25.00ml @15:24:12.28 Vacuum too high -- waiting up to 40s @15:24:12.77 Intake @47.9psia, vacuum from 20.0 to 24.0psi @15:24:12.79 SS.reconfigure forSampling @15:24:12.82 SS.seek 25.00ml @15:24:14.12 Vacuum too high -- waiting up to 40s @15:24:14.62 SS.seek 25.00ml @15:24:16.00 Vacuum too high -- waiting up to 40s @15:24:16.48 Intake @47.7psia, vacuum from 20.0 to 24.0psi @15:24:16.49 SS.reconfigure forSampling @15:24:16.53 SS.seek 25.00ml @15:24:17.87 Vacuum too high -- waiting up to 40s @15:24:18.36 Intake @47.7psia, vacuum from 20.0 to 24.0psi @15:24:18.38 SS.reconfigure forSampling @15:24:18.41 SS.seek 25.00ml @15:24:19.87 Vacuum too high -- waiting up to 40s @15:24:21.13 Intake @49.0psia, vacuum from 20.0 to 24.0psi @15:24:21.15 SS.reconfigure forSampling @15:24:21.18 SS.seek 25.00ml @15:24:22.93 Vacuum too high -- waiting up to 40s @15:24:23.42 Intake @48.4psia, vacuum from 20.0 to 24.0psi @15:24:23.44 SS.reconfigure forSampling @15:24:23.47 SS.seek 25.00ml @15:24:24.78 Vacuum too high -- waiting up to 40s @15:24:25.27 Intake @48.1psia, vacuum from 20.0 to 24.0psi @15:24:25.30 SS.reconfigure forSampling @15:24:25.33 SS.seek 25.00ml @15:24:26.65 Vacuum too high -- waiting up to 40s @15:24:27.14 Intake @47.9psia, vacuum from 20.0 to 24.0psi @15:24:27.16 SS.reconfigure forSampling @15:24:27.19 SS.seek 25.00ml @15:24:28.49 Vacuum too high -- waiting up to 40s @15:24:28.99 SS.seek 25.00ml @15:24:30.24 Vacuum too high -- waiting up to 40s @15:24:30.74 SS.seek 25.00ml @15:24:31.87 Vacuum too high -- waiting up to 40s @15:24:32.36 Intake @47.7psia, vacuum from 20.0 to 24.0psi @15:24:32.38 SS.reconfigure forSampling @15:24:32.41 SS.seek 25.00ml @15:24:33.65 Vacuum too high -- waiting up to 40s @15:24:34.35 Intake @48.8psia, vacuum from 20.0 to 24.0psi @15:24:34.37 SS.reconfigure forSampling @15:24:34.40 SS.seek 25.00ml @15:24:35.68 Vacuum too high -- waiting up to 40s @15:24:36.17 Intake @48.6psia, vacuum from 20.0 to 24.0psi @15:24:36.18 SS.reconfigure forSampling @15:24:36.22 SS.seek 25.00ml @15:24:37.43 Vacuum too high -- waiting up to 40s @15:24:37.91 Intake @48.2psia, vacuum from 20.0 to 24.0psi @15:24:37.93 SS.reconfigure forSampling @15:24:37.96 SS.seek 25.00ml @15:24:39.15 Vacuum too high -- waiting up to 40s @15:24:39.65 SS.seek 25.00ml @15:24:40.89 Vacuum too high -- waiting up to 40s @15:24:41.37 Intake @48.0psia, vacuum from 20.0 to 24.0psi @15:24:41.38 SS.reconfigure forSampling @15:24:41.42 SS.seek 25.00ml @15:24:42.62 Vacuum too high -- waiting up to 40s @15:24:43.11 Intake @48.1psia, vacuum from 20.0 to 24.0psi @15:24:43.13 SS.reconfigure forSampling @15:24:43.16 SS.seek 25.00ml @15:24:44.31 Vacuum too high -- waiting up to 40s @15:24:44.80 Intake @48.2psia, vacuum from 20.0 to 24.0psi @15:24:44.81 SS.reconfigure forSampling @15:24:44.85 SS.seek 25.00ml @15:24:46.09 Vacuum too high -- waiting up to 40s @15:24:46.57 Intake @48.3psia, vacuum from 20.0 to 24.0psi @15:24:46.58 SS.reconfigure forSampling @15:24:46.62 SS.seek 25.00ml @15:24:47.81 Vacuum too high -- waiting up to 40s @15:24:48.30 Intake @48.7psia, vacuum from 20.0 to 24.0psi @15:24:48.31 SS.reconfigure forSampling @15:24:48.34 SS.seek 25.00ml @15:24:49.49 Vacuum too high -- waiting up to 40s @15:24:49.97 Intake @48.1psia, vacuum from 20.0 to 24.0psi @15:24:49.98 SS.reconfigure forSampling @15:24:50.02 SS.seek 25.00ml @15:24:51.27 Vacuum too high -- waiting up to 40s @15:24:51.76 SS.seek 25.00ml @15:24:52.99 Vacuum too high -- waiting up to 40s @15:24:54.09 Intake @48.1psia, vacuum from 20.0 to 24.0psi @15:24:54.10 SS.reconfigure forSampling @15:24:54.13 SS.seek 25.00ml @15:24:55.81 Vacuum too high -- waiting up to 40s @15:24:56.31 SS.seek 25.00ml @15:24:57.43 Vacuum too high -- waiting up to 40s @15:24:57.92 Intake @47.8psia, vacuum from 20.0 to 24.0psi @15:24:57.94 SS.reconfigure forSampling @15:24:57.97 SS.seek 25.00ml @15:24:59.12 Vacuum too high -- waiting up to 40s @15:24:59.61 Intake @48.3psia, vacuum from 20.0 to 24.0psi @15:24:59.63 SS.reconfigure forSampling @15:24:59.66 SS.seek 25.00ml @15:25:00.71 Vacuum too high -- waiting up to 40s @15:25:01.20 Intake @48.7psia, vacuum from 20.0 to 24.0psi @15:25:01.22 SS.reconfigure forSampling @15:25:01.25 SS.seek 25.00ml @15:25:02.31 Vacuum too high -- waiting up to 40s @15:25:02.80 Intake @48.0psia, vacuum from 20.0 to 24.0psi @15:25:02.82 SS.reconfigure forSampling @15:25:02.85 SS.seek 25.00ml @15:25:04.24 Vacuum too high -- waiting up to 40s @15:25:04.96 SS.seek 25.00ml @15:25:06.24 Vacuum too high -- waiting up to 40s @15:25:06.73 Intake @47.8psia, vacuum from 20.0 to 24.0psi @15:25:06.75 SS.reconfigure forSampling @15:25:06.78 SS.seek 25.00ml @15:25:08.02 Vacuum too high -- waiting up to 40s @15:25:08.73 Intake @48.5psia, vacuum from 20.0 to 24.0psi @15:25:08.75 SS.reconfigure forSampling @15:25:08.78 SS.seek 25.00ml @15:25:10.09 Vacuum too high -- waiting up to 40s @15:25:10.59 Intake @48.6psia, vacuum from 20.0 to 24.0psi @15:25:10.61 SS.reconfigure forSampling @15:25:10.64 SS.seek 25.00ml @15:25:11.68 Vacuum too high -- waiting up to 40s @15:25:12.28 Intake @48.0psia, vacuum from 20.0 to 24.0psi @15:25:12.29 SS.reconfigure forSampling @15:25:12.33 SS.seek 25.00ml @15:25:13.71 Vacuum too high -- waiting up to 40s @15:25:14.19 Intake @47.9psia, vacuum from 20.0 to 24.0psi @15:25:14.21 SS.reconfigure forSampling @15:25:14.24 SS.seek 25.00ml @15:25:15.43 Vacuum too high -- waiting up to 40s @15:25:16.12 Intake @48.1psia, vacuum from 20.0 to 24.0psi @15:25:16.14 SS.reconfigure forSampling @15:25:16.17 SS.seek 25.00ml @15:25:17.46 Vacuum too high -- waiting up to 40s @15:25:17.95 Intake @48.2psia, vacuum from 20.0 to 24.0psi @15:25:17.96 SS.reconfigure forSampling @15:25:18.00 SS.seek 25.00ml @15:25:19.20 Vacuum too high -- waiting up to 40s @15:25:19.89 Intake @48.9psia, vacuum from 20.0 to 24.0psi @15:25:19.91 SS.reconfigure forSampling @15:25:19.94 SS.seek 25.00ml @15:25:21.09 Vacuum too high -- waiting up to 40s @15:25:21.58 Intake @48.4psia, vacuum from 20.0 to 24.0psi @15:25:21.59 SS.reconfigure forSampling @15:25:21.63 SS.seek 25.00ml @15:25:22.80 Vacuum too high -- waiting up to 40s @15:25:23.28 Intake @48.2psia, vacuum from 20.0 to 24.0psi @15:25:23.30 SS.reconfigure forSampling @15:25:23.33 SS.seek 25.00ml @15:25:24.52 Vacuum too high -- waiting up to 40s @15:25:25.22 Intake @48.2psia, vacuum from 20.0 to 24.0psi @15:25:25.84 SS.reconfigure forSampling @15:25:25.87 SS.seek 25.00ml @15:25:27.55 Vacuum too high -- waiting up to 40s @15:25:28.26 Intake @48.3psia, vacuum from 20.0 to 24.0psi @15:25:28.28 SS.reconfigure forSampling @15:25:28.31 SS.seek 25.00ml @15:25:29.55 Vacuum too high -- waiting up to 40s @15:25:30.26 Intake @48.5psia, vacuum from 20.0 to 24.0psi @15:25:30.28 SS.reconfigure forSampling @15:25:30.31 SS.seek 25.00ml @15:25:31.71 Vacuum too high -- waiting up to 40s @15:25:32.42 Intake @49.0psia, vacuum from 20.0 to 24.0psi @15:25:32.44 SS.reconfigure forSampling @15:25:32.47 SS.seek 25.00ml @15:25:33.80 Vacuum too high -- waiting up to 40s @15:25:34.29 Intake @48.4psia, vacuum from 20.0 to 24.0psi @15:25:34.30 SS.reconfigure forSampling @15:25:34.34 SS.seek 25.00ml @15:25:35.46 Vacuum too high -- waiting up to 40s @15:25:36.17 Intake @48.4psia, vacuum from 20.0 to 24.0psi @15:25:36.18 SS.reconfigure forSampling @15:25:36.22 SS.seek 25.00ml @15:25:37.49 Vacuum too high -- waiting up to 40s @15:25:37.97 Intake @48.2psia, vacuum from 20.0 to 24.0psi @15:25:37.99 SS.reconfigure forSampling @15:25:38.02 SS.seek 25.00ml @15:25:39.08 Vacuum too high -- waiting up to 40s @15:25:39.79 Intake @48.1psia, vacuum from 20.0 to 24.0psi @15:25:39.80 SS.reconfigure forSampling @15:25:39.84 SS.seek 25.00ml @15:25:41.08 Vacuum too high -- waiting up to 40s @15:25:41.79 Intake @48.3psia, vacuum from 20.0 to 24.0psi @15:25:41.81 SS.reconfigure forSampling @15:25:41.84 SS.seek 25.00ml @15:25:43.08 Vacuum too high -- waiting up to 40s @15:25:43.57 Intake @48.2psia, vacuum from 20.0 to 24.0psi @15:25:43.58 SS.reconfigure forSampling @15:25:43.62 SS.seek 25.00ml @15:25:44.77 Vacuum too high -- waiting up to 40s @15:25:45.47 Intake @48.7psia, vacuum from 20.0 to 24.0psi @15:25:45.48 SS.reconfigure forSampling @15:25:45.52 SS.seek 25.00ml @15:25:46.71 Vacuum too high -- waiting up to 40s @15:25:47.19 Intake @48.9psia, vacuum from 20.0 to 24.0psi @15:25:47.20 SS.reconfigure forSampling @15:25:47.24 SS.seek 25.00ml @15:25:48.21 Vacuum too high -- waiting up to 40s @15:25:48.70 Intake @48.2psia, vacuum from 20.0 to 24.0psi @15:25:48.72 SS.reconfigure forSampling @15:25:48.75 SS.seek 25.00ml @15:25:48.97 delay 20 seconds @15:25:49.90 Vacuum too high -- waiting up to 40s @15:25:50.39 Intake @48.2psia, vacuum from 20.0 to 24.0psi @15:25:50.41 SS.reconfigure forSampling @15:25:50.44 SS.seek 25.00ml @15:25:51.52 Vacuum too high -- waiting up to 40s @15:25:52.23 SS.seek 25.00ml @15:25:53.40 Vacuum too high -- waiting up to 40s @15:25:54.10 Intake @48.1psia, vacuum from 20.0 to 24.0psi @15:25:54.12 SS.reconfigure forSampling @15:25:54.15 SS.seek 25.00ml @15:25:55.36 Vacuum too high -- waiting up to 40s @15:25:55.84 Intake @47.8psia, vacuum from 20.0 to 24.0psi @15:25:55.86 SS.reconfigure forSampling @15:25:56.50 SS.seek 25.00ml @15:25:58.30 Vacuum too high -- waiting up to 40s @15:25:59.01 Intake @48.8psia, vacuum from 20.0 to 24.0psi @15:25:59.03 SS.reconfigure forSampling @15:25:59.06 SS.seek 25.00ml @15:26:00.08 Vacuum too high -- waiting up to 40s @15:26:00.79 Intake @48.6psia, vacuum from 20.0 to 24.0psi @15:26:00.81 SS.reconfigure forSampling @15:26:00.84 SS.seek 25.00ml @15:26:01.93 Vacuum too high -- waiting up to 40s @15:26:02.42 Intake @48.2psia, vacuum from 20.0 to 24.0psi @15:26:02.44 SS.reconfigure forSampling @15:26:02.47 SS.seek 25.00ml @15:26:03.65 Vacuum too high -- waiting up to 40s @15:26:04.14 Intake @48.3psia, vacuum from 20.0 to 24.0psi @15:26:04.16 SS.reconfigure forSampling @15:26:04.19 SS.seek 25.00ml @15:26:05.31 Vacuum too high -- waiting up to 40s @15:26:06.01 Intake @48.5psia, vacuum from 20.0 to 24.0psi @15:26:06.03 SS.reconfigure forSampling @15:26:06.06 SS.seek 25.00ml @15:26:07.11 Vacuum too high -- waiting up to 40s @15:26:07.81 Intake @48.3psia, vacuum from 20.0 to 24.0psi @15:26:07.83 SS.reconfigure forSampling @15:26:07.86 SS.seek 25.00ml @15:26:08.93 Vacuum too high -- waiting up to 40s @15:26:08.98 ATV.dial :air3 @15:26:09.42 Intake @48.4psia, vacuum from 20.0 to 24.0psi @15:26:09.44 SS.reconfigure forSampling @15:26:09.47 SS.seek 25.00ml @15:26:10.16 AS.seek 0.590ml @15:26:10.58 Vacuum too high -- waiting up to 40s @15:26:11.29 Intake @48.5psia, vacuum from 20.0 to 24.0psi @15:26:11.31 SS.reconfigure forSampling @15:26:11.34 SS.seek 25.00ml @15:26:12.65 Vacuum too high -- waiting up to 40s @15:26:13.36 Intake @48.6psia, vacuum from 20.0 to 24.0psi @15:26:13.37 SS.reconfigure forSampling @15:26:13.41 SS.seek 25.00ml @15:26:14.68 Vacuum too high -- waiting up to 40s @15:26:15.39 Intake @48.8psia, vacuum from 20.0 to 24.0psi @15:26:15.40 SS.reconfigure forSampling @15:26:15.44 SS.seek 25.00ml @15:26:16.52 Vacuum too high -- waiting up to 40s @15:26:17.00 Intake @48.5psia, vacuum from 20.0 to 24.0psi @15:26:17.02 SS.reconfigure forSampling @15:26:17.05 SS.seek 25.00ml @15:26:18.08 Vacuum too high -- waiting up to 40s @15:26:18.57 Intake @48.1psia, vacuum from 20.0 to 24.0psi @15:26:18.58 SS.reconfigure forSampling @15:26:18.62 SS.seek 25.00ml @15:26:19.61 Vacuum too high -- waiting up to 40s @15:26:20.38 SS.seek 25.00ml @15:26:21.43 Vacuum too high -- waiting up to 40s @15:26:21.92 Intake @48.1psia, vacuum from 20.0 to 24.0psi @15:26:21.94 SS.reconfigure forSampling @15:26:21.97 SS.seek 25.00ml @15:26:23.11 Vacuum too high -- waiting up to 40s @15:26:23.81 Intake @48.3psia, vacuum from 20.0 to 24.0psi @15:26:23.82 SS.reconfigure forSampling @15:26:23.86 SS.seek 25.00ml @15:26:24.93 Vacuum too high -- waiting up to 40s @15:26:26.04 Intake @48.2psia, vacuum from 20.0 to 24.0psi @15:26:26.05 SS.reconfigure forSampling @15:26:26.09 SS.seek 25.00ml @15:26:27.64 Vacuum too high -- waiting up to 40s @15:26:28.35 Intake @48.3psia, vacuum from 20.0 to 24.0psi @15:26:28.37 SS.reconfigure forSampling @15:26:28.40 SS.seek 25.00ml @15:26:29.64 Vacuum too high -- waiting up to 40s @15:26:30.35 Intake @48.2psia, vacuum from 20.0 to 24.0psi @15:26:30.37 SS.reconfigure forSampling @15:26:30.40 SS.seek 25.00ml @15:26:31.74 Vacuum too high -- waiting up to 40s @15:26:32.45 Intake @48.3psia, vacuum from 20.0 to 24.0psi @15:26:32.47 SS.reconfigure forSampling @15:26:32.50 SS.seek 25.00ml @15:26:33.77 Vacuum too high -- waiting up to 40s @15:26:34.48 Intake @48.3psia, vacuum from 20.0 to 24.0psi @15:26:34.50 SS.reconfigure forSampling @15:26:34.53 SS.seek 25.00ml @15:26:35.61 Vacuum too high -- waiting up to 40s @15:26:36.32 Intake @48.2psia, vacuum from 20.0 to 24.0psi @15:26:36.34 SS.reconfigure forSampling @15:26:36.37 SS.seek 25.00ml @15:26:37.49 Vacuum too high -- waiting up to 40s @15:26:38.20 Intake @48.4psia, vacuum from 20.0 to 24.0psi @15:26:38.22 SS.reconfigure forSampling @15:26:38.25 SS.seek 25.00ml @15:26:39.30 Vacuum too high -- waiting up to 40s @15:26:39.78 Intake @48.1psia, vacuum from 20.0 to 24.0psi @15:26:39.80 SS.reconfigure forSampling @15:26:39.83 SS.seek 25.00ml @15:26:40.07 SS.reconfigure SSconfig @15:26:40.10 SS.hold @15:26:40.26 Intake.select :close @15:26:40.41 ShallowSampler::Clogged in WCR -- progress too slow after 474.1ml @15:26:40.42 SSV.dial :exhaust @15:26:41.22 Exhaust.select :open @15:26:41.32 SS.reconfigure SSexhale @15:26:41.36 SS.seek 0.00ml @15:26:50.18 Exhaust.select :close @15:26:50.29 Sampled 474.1ml @15:26:50.30 collectSample: Complete @15:26:50.31 Drying puck before fixing for 04:00.00 @15:26:50.32 CBV.dial :waste2b @15:26:51.10 delay 1:00 @15:26:53.28 Sleepy.power :sampler,:OFF @15:27:51.10 CSV.dial :air @15:27:51.56 CS.reconfigure CSfast @15:27:51.59 CS.seek 10.000ml @15:28:10.81 delay 3 seconds @15:28:13.82 ATV.dial :ASV @15:28:14.99 ASV.dial :wasteR @15:28:15.05 AS.seek 0.090ml @15:28:15.48 CBV.dial between CSV and waste2b @15:28:15.79 Pumping wcr puck from tube 3 up to 55psi... @15:28:15.84 CBV.dial SSV @15:28:17.26 CBV.dial between CSV and waste2b @15:28:18.19 CSV.dial between air and CTV @15:28:18.59 CS.seek 6.710ml @15:28:26.80 CTV.dial CSV,avoiding => air @15:28:26.82 CSV.dial CTV @15:28:27.55 CS.reconfigure CSpressurize @15:28:27.58 CS.seek 0.000ml @15:28:45.14 CSV.dial :air @15:28:45.66 CS.reconfigure CSfast @15:28:45.69 CS.seek 10.000ml @15:29:09.27 CSV.dial between air and CTV @15:29:10.66 CS.seek 1.998ml @15:29:29.58 CTV.dial CSV,avoiding => air @15:29:29.59 CSV.dial CTV @15:29:29.96 CS.reconfigure CSpressurize @15:29:29.99 CS.seek 0.000ml @15:29:30.04 CBV.dial :waste2b @15:29:30.36 CS.reconfigure CSconfig @15:29:30.39 CS.seek empty @15:29:36.23 delay 1:00 @15:29:57.29 delay 30 seconds @15:30:27.30 ATV.dial :extSrc @15:30:27.80 delay 3 seconds @15:30:31.37 ATV.dial :ElutionWater @15:30:32.29 AS.seek 0.120ml @15:30:36.24 CTV.dial :air,avoiding => intake @15:30:37.52 Fix #1 of 2 (with rnal) for 02:00.00 @15:30:37.53 CS Pulling 2ml of rnal CSV.dial CSR @15:30:37.96 CSR.select :rnal @15:30:38.06 CS.seek 2.000ml @15:30:38.64 ATV.dial :air3 @15:30:39.02 AS.seek 0.130ml @15:30:41.32 delay 2 seconds @15:30:43.33 ATV.dial :bleach @15:30:43.80 AS.seek 0.230ml @15:30:43.94 delay 5 seconds @15:30:48.95 CSR.select :air @15:30:49.04 CS.reconfigure CSfast @15:30:49.08 CS.seek 10.000ml @15:31:04.46 delay 3 seconds @15:31:07.47 ATV.dial :air3 @15:31:07.80 delay 3 seconds @15:31:08.04 CBV.dial between CSV and waste2b @15:31:08.37 Pumping wcr puck from tube 3 up to 55psi... @15:31:08.42 CBV.dial SSV @15:31:09.84 CBV.dial between CSV and waste2b @15:31:10.77 CSV.dial between air and CTV @15:31:10.86 AS.seek 0.255ml @15:31:11.42 CS.seek 6.596ml @15:31:16.19 delay 3 seconds @15:31:19.20 ATV.dial :bleach @15:31:19.60 AS.seek 0.240ml @15:31:19.92 CTV.dial CSV,avoiding => air @15:31:19.94 CSV.dial CTV @15:31:21.30 CS.reconfigure CSpressurize @15:31:21.33 CS.seek 0.000ml @15:31:22.91 delay 3 seconds @15:31:25.92 ATV.dial :ASV @15:31:26.80 ASV.dial :SPEdelivery @15:31:27.11 AFV.select :carrierSolution @15:31:27.21 delay 2 seconds @15:31:29.22 AS.reconfigure ASconfig @15:31:29.25 AS.seek 0.500ml @15:31:37.59 delay 2:00 @15:31:44.69 delay 1 second @15:31:45.70 AFV.select :holdingCoil @15:31:45.86 AS.reconfigure ASslow1 @15:31:45.89 AS.seek 0.170ml @15:32:53.47 SPE.hold 2:46:40,95.0C @15:32:53.49 delay 3:00 @15:33:37.60 CBV.dial :waste2b @15:33:37.95 CS.reconfigure CSconfig @15:33:37.98 CS.seek empty @15:33:39.65 delay 1:00 @15:34:39.66 CTV.dial :air,avoiding => intake @15:34:40.99 Fix #2 of 2 (with rnal) for 02:00.00 @15:34:41.00 CS Pulling 2ml of rnal CSV.dial CSR @15:34:41.51 CSR.select :rnal @15:34:41.61 CS.seek 2.000ml @15:34:47.59 delay 5 seconds @15:34:52.60 CSR.select :air @15:34:52.69 CS.reconfigure CSfast @15:34:52.73 CS.seek 10.000ml @15:35:12.43 CBV.dial between CSV and waste2b @15:35:12.77 Pumping wcr puck from tube 3 up to 55psi... @15:35:12.81 CBV.dial SSV @15:35:14.27 CBV.dial between CSV and waste2b @15:35:15.23 CSV.dial between air and CTV @15:35:15.91 CS.seek 6.476ml @15:35:25.03 CTV.dial CSV,avoiding => air @15:35:25.05 CSV.dial CTV @15:35:26.39 CS.reconfigure CSpressurize @15:35:26.42 CS.seek 0.000ml @15:35:42.36 delay 2:00 @15:35:53.50 AFV.select :carrierSolution @15:35:53.59 delay 2 seconds @15:35:55.60 AS.reconfigure ASconfig @15:35:55.63 AS.seek 1.000ml @15:36:44.30 delay 1 second @15:36:45.31 AFV.select :holdingCoil @15:36:45.40 delay 1 second @15:36:46.41 ASV.dial :wasteD @15:36:47.42 AS.seek 0.000ml @15:37:42.37 CBV.dial :waste2b @15:37:42.72 CS.reconfigure CSconfig @15:37:42.75 CS.seek empty @15:37:44.39 delay 1:00 @15:37:46.02 Priming Eluate @15:37:46.03 AFV.select :carrierSolution @15:37:46.12 delay 2 seconds @15:37:48.13 AS.seek 0.500ml @15:38:17.58 delay 1 second @15:38:18.59 AFV.select :holdingCoil @15:38:18.68 ATV.dial ASV @15:38:18.69 ASV.dial :SPErecovery @15:38:19.33 Swish #1 of 2 @15:38:19.39 AS.reconfigure ASslow0 @15:38:19.42 AS.seek 0.600ml @15:38:29.95 AS.seek 0.500ml @15:38:40.44 Swish #2 of 2 @15:38:40.50 AS.seek 0.600ml @15:38:44.41 CTV.dial :air,avoiding => intake @15:38:46.24 Storing wcr puck in Collection Clamp to tube 2 @15:38:46.38 SC.reconfigure SCtight @15:38:46.41 SC.seek 2 @15:38:48.59 SC.reconfigure SCloose @15:38:48.77 Elbow.reconfigure TightElbow @15:38:48.80 Elbow.seek 2 @15:38:50.42 Forearm.reconfigure TightForearm @15:38:50.45 Forearm.seek 2 + 800 counts @15:38:51.08 AS.seek 0.500ml @15:38:51.59 SE.reconfigure SEpick @15:38:51.64 SE.seek beamBreak @15:38:53.71 SE.configure SEconfig @15:38:53.83 Elbow.reconfigure LimpElbow @15:38:53.87 Forearm.reconfigure LooseForearm @15:38:53.91 Forearm.seek 2 @15:38:54.12 Elbow.hold @15:38:54.17 Elbow.reconfigure TightElbow @15:38:54.25 SE.seek 9.03 Pucks below Top Plate @15:38:54.70 Forearm.coast @15:38:54.73 Forearm.seek 2 + 322 counts @15:38:55.02 Forearm.reconfigure TightForearm @15:38:55.06 Forearm.seek 2 + 800 counts @15:38:55.32 SE.seek 10.03 Pucks below Top Plate @15:38:55.85 Forearm.reconfigure LooseForearm @15:38:55.88 Forearm.seek retract @15:38:56.80 Elbow.seek CC @15:38:58.39 Elbow.reconfigure LooseElbow @15:38:58.47 Forearm.seek CC @15:38:59.39 CC.seek sealed @15:39:00.34 Hand.grip @15:39:01.11 CC.seek open @15:39:01.56 delay 3 seconds @15:39:04.48 Forearm.seek retract @15:39:04.62 AS.reconfigure ASslow1 @15:39:04.65 AS.seek 0.676ml @15:39:05.44 Elbow.reconfigure TightElbow @15:39:05.47 Elbow.seek 2 @15:39:07.05 Elbow.reconfigure LimpElbow @15:39:07.09 Forearm.seek 2 @15:39:08.06 Elbow.hold @15:39:08.12 Elbow.reconfigure TightElbow @15:39:08.16 Hand.release @15:39:08.94 Forearm.coast @15:39:08.98 Forearm.seek 2 + 336 counts @15:39:09.27 Forearm.reconfigure TightForearm @15:39:09.30 Forearm.seek 2 + 800 counts @15:39:09.51 SE.seek down @15:39:10.61 Forearm.reconfigure LooseForearm @15:39:10.65 Forearm.seek retract @15:39:11.22 SE.coast @15:39:11.56 SC.coast @15:39:11.59 Starting finalPurge @15:39:11.60 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:39:11.75 Elbow.seek FlushPuck::Garage @15:39:13.54 Elbow.reconfigure LooseElbow @15:39:13.62 Forearm.seek garage @15:39:14.63 Hand.grip @15:39:16.05 Forearm.seek retract @15:39:17.08 Elbow.reconfigure TightElbow @15:39:17.11 Elbow.seek CC @15:39:19.87 Elbow.reconfigure LooseElbow @15:39:19.95 Forearm.reconfigure TightForearm @15:39:19.98 Forearm.seek CC @15:39:21.12 CC.seek closed @15:39:24.89 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 2.10 @15:39:24.94 Hand.release @15:39:25.78 Forearm.reconfigure LooseForearm @15:39:25.81 Forearm.seek retract @15:39:26.76 Flushing the Collection Valve::Manifold @15:39:26.86 CS Pulling 5ml of flush CSV.dial CSR @15:39:27.29 CSR.select :flush @15:39:27.39 CS.seek 5.000ml @15:39:40.85 delay 3 seconds @15:39:40.93
delay 5 seconds @15:39:43.86 Eluate primed @15:39:43.87 ATV.dial :waste2 @15:39:44.40 AS.reconfigure ASnorm @15:39:44.43 AS.seek 0.000ml @15:39:45.94
CSR.select :air @15:39:46.08 CS.reconfigure CSfast @15:39:46.11 CS.seek 7.500ml @15:39:52.77 CSV.dial :waste @15:39:54.05 CS.reconfigure CSconfig @15:39:54.08 CS.seek 0.000ml @15:40:13.90 Flushing lower manifold's waste1 port @15:40:13.99 CSV.dial :flush @15:40:14.44 CS.reconfigure CSfast @15:40:14.47 CS.seek 5.000ml @15:40:19.29 SPE.coast @15:40:19.36 ASV.dial :SPErecovery @15:40:19.38 Setting up PCR for HF183em -- writing /var/log/esp/HF183em14mar1415h499ml.pcr @15:40:19.39 Building PCR Slug from ASV with MM2 mix, PP4 primer @15:40:19.40 Priming with carrier @15:40:19.41 ATV.dial ARV @15:40:20.12 ARV.dial :carrier @15:40:20.40 AS.seek 0.500ml @15:40:26.77
delay 5 seconds @15:40:31.78 CSV.dial :air @15:40:32.26 CS.seek 7.000ml @15:40:37.71 CSV.dial CTV @15:40:37.73
CTV.dial CSV @15:40:39.03 CBV.dial :waste1 @15:40:39.78 CS.seek 0.000ml @15:40:46.27 AFV.select :carrierSolution @15:40:46.38 delay 2 seconds @15:40:48.39 AS.reconfigure ASconfig @15:40:48.43 AS.seek 1.000ml @15:40:56.18
Flushing lower manifold's waste2b port @15:40:56.27 CSV.dial :flush @15:40:57.06 CS.seek 5.000ml @15:41:09.21 delay 5 seconds @15:41:14.22 CSV.dial :air @15:41:14.77 CS.seek 7.000ml @15:41:17.88 delay 1 second @15:41:18.89 AFV.select :holdingCoil @15:41:18.98 delay 1 second @15:41:19.99 ATV.dial :waste @15:41:20.17 CSV.dial CTV @15:41:20.19
CTV.dial CSV @15:41:20.62 CBV.dial :waste2b @15:41:21.25 AS.reconfigure ASnorm @15:41:21.28 AS.seek empty @15:41:21.36
CS.seek 0.000ml @15:41:37.77 Flushing upper manifold @15:41:37.86 CSV.dial :flush @15:41:38.65 CS.seek 5.000ml @15:41:50.73 delay 5 seconds @15:41:55.74 CSV.dial :air @15:41:56.28 CS.seek 7.000ml @15:42:01.67 CSV.dial CBV @15:42:01.69
CBV.dial CSV @15:42:02.81 CTV.dial :air @15:42:04.09 CS.seek 0.000ml @15:42:12.76 AFV.select :carrierSolution @15:42:12.92 delay 2 seconds @15:42:14.93 AS.reconfigure ASconfig @15:42:14.96 AS.seek 0.500ml @15:42:20.36
delay 7 seconds @15:42:27.37 CS.seek 3.000ml @15:42:35.36 CSV.dial :waste @15:42:35.87 CS.seek 0.000ml @15:42:43.47 CSV.dial :flush @15:42:43.91 CS.seek 3.000ml @15:42:44.44 delay 1 second @15:42:45.45 AFV.select :holdingCoil @15:42:45.54 Building Slug @15:42:45.55 ATV.dial :ARV @15:42:46.81 delay 2 seconds @15:42:48.81 ARV.dial :air1 @15:42:49.13 delay 2 seconds @15:42:51.19 AS.reconfigure ASslow2 @15:42:51.22 AS.seek 0.506ml @15:42:51.42
delay 5 seconds @15:42:56.43 CSV.dial :air @15:42:56.91 CS.seek 5.000ml @15:42:57.52 delay 2 seconds @15:42:59.53 ARV.dial :MM2 @15:43:00.85 delay 2 seconds @15:43:02.26
CSV.dial :air @15:43:02.28 CS.seek 0.000ml @15:43:02.86 Adding MM2 @15:43:02.92 AS.seek 0.524ml @15:43:14.08
delay 7 seconds @15:43:21.09 CS.seek 3.000ml @15:43:21.41 delay 25 seconds @15:43:28.67
CSV.dial :waste @15:43:29.46 CS.seek 0.000ml @15:43:36.89 Disinfecting external Intake with mfbkill @15:43:36.99 CSV.dial CSR @15:43:38.23 CSR.select :mfbkill @15:43:38.33 CS.seek 8.000ml @15:43:46.42 ARV.dial :PP4 @15:43:46.75 Adding PP4 @15:43:46.76 delay 2 seconds @15:43:48.82 AS.seek 0.530ml @15:43:55.10 delay 10 seconds @15:43:56.89
delay 5 seconds @15:44:01.90 CSR.select :air @15:44:01.99 CS.seek 10.000ml @15:44:05.11 ARV.dial :air3 @15:44:05.42 adding ARV air @15:44:05.43 delay 2 seconds @15:44:07.32 CSV.dial CBV @15:44:07.34
CBV.dial CSV @15:44:07.49 AS.seek 0.536ml @15:44:08.40
CTV.dial :intake @15:44:09.10 Intake.select :open @15:44:09.20 CS.seek 2.000ml @15:44:13.83 delay 5 seconds @15:44:18.84 ARV.dial :carrier @15:44:19.88 delay 7 seconds @15:44:26.94 AS.seek 0.594ml @15:44:27.95
Intake.select :close @15:44:28.05 CTV.dial :air @15:44:28.83 CS.reconfigure CSconfig @15:44:28.86 CS.seek 0.000ml @15:44:34.76 Flushing lower manifold's waste1 port @15:44:34.77 CS Pulling 4ml of mfbkill @15:44:34.78 CSV.dial CSR @15:44:35.82 CSR.select :mfbkill @15:44:35.92 CS.seek 4.000ml @15:44:46.91 delay 5 seconds @15:44:51.92 CSR.select :air @15:44:52.01 CS.reconfigure CSfast @15:44:52.05 CS.seek 10.000ml @15:45:06.13 CSV.dial CTV @15:45:06.15
CTV.dial CSV @15:45:07.46 CBV.dial :waste1 @15:45:07.91 CS.seek 0.000ml @15:45:26.44 delay 5 seconds @15:45:30.80
Flushing lower manifold's waste2b port @15:45:30.81 CS Pulling 4ml of mfbkill CSV.dial CSR @15:45:31.32 CSR.select :mfbkill @15:45:31.42 CS.reconfigure CSconfig @15:45:31.45 ATV.dial :ASV @15:45:31.47
CS.seek 4.000ml @15:45:31.82 delay 2 seconds @15:45:33.87 AS.seek 0.600ml @15:45:40.37 delay 4 seconds @15:45:42.47
delay 5 seconds @15:45:44.38 ATV.dial ARV @15:45:44.70 delay 2 seconds @15:45:46.71 ARV.dial :carrier @15:45:46.72 delay 2 seconds @15:45:47.48
CSR.select :air @15:45:47.57 CS.reconfigure CSfast @15:45:47.61 CS.seek 10.000ml @15:45:48.77 AS.seek 0.650ml @15:46:01.69 CSV.dial CTV @15:46:01.71
CTV.dial CSV @15:46:02.22 CBV.dial :waste2b @15:46:02.97 CS.seek 0.000ml @15:46:25.91 Killing upper manifold @15:46:25.92 CS Pulling 4ml of mfbkill @15:46:25.93 CSV.dial CSR @15:46:26.42 CSR.select :mfbkill @15:46:26.52 CS.reconfigure CSconfig @15:46:26.56 CS.seek 4.000ml @15:46:37.47 delay 5 seconds @15:46:40.08 delay 4 seconds @15:46:42.48
CSR.select :air @15:46:42.57 CS.reconfigure CSfast @15:46:42.61 CS.seek 10.000ml @15:46:44.09 ATV.dial PCR @15:46:44.70 ASV.dial :waste1 @15:46:45.59 ARV.dial :air1 @15:46:46.02 Positioning Slug by 0.324ml @15:46:46.07 AS.seek 0.326ml @15:46:57.38 CSV.dial CBV @15:46:57.40
CBV.dial CSV @15:46:58.41 CTV.dial :air @15:46:59.68 CS.seek 0.000ml @15:47:22.50 delay 7 seconds @15:47:29.51 CS.seek 3.000ml @15:47:37.00 CSV.dial :waste @15:47:37.43 CS.seek 0.000ml @15:47:44.97 Flushing upper manifold @15:47:45.06 CSV.dial :flush @15:47:45.49 CS.seek 5.000ml @15:47:57.41 delay 5 seconds @15:48:02.42 CSV.dial :air @15:48:02.91 CS.seek 7.000ml @15:48:08.28 CSV.dial CBV @15:48:08.30
CBV.dial CSV @15:48:09.38 CTV.dial :air @15:48:09.39 CS.seek 0.000ml @15:48:25.72 delay 7 seconds @15:48:32.73 CS.seek 3.000ml @15:48:40.31 CSV.dial :waste @15:48:40.82 CS.seek 0.000ml @15:48:48.58 CSV.dial :flush @15:48:49.02 CS.seek 3.000ml @15:48:56.50 delay 5 seconds @15:49:01.51 CSV.dial :air @15:49:01.98 CS.seek 5.000ml @15:49:07.25 CSV.dial :air @15:49:07.27 CS.seek 0.000ml @15:49:19.15 delay 7 seconds @15:49:26.16 CS.seek 3.000ml @15:49:33.74 CSV.dial :waste @15:49:34.54 CS.seek 0.000ml @15:49:41.99 Flushing lower manifold's waste1 port @15:49:42.08 CSV.dial :flush @15:49:42.60 CS.seek 5.000ml @15:49:54.46 delay 5 seconds @15:49:59.47 CSV.dial :air @15:49:59.93 CS.seek 7.000ml @15:50:05.31 CSV.dial CTV @15:50:05.33
CTV.dial CSV @15:50:06.63 CBV.dial :waste1 @15:50:07.08 CS.seek 0.000ml @15:50:23.37 Flushing lower manifold's waste2b port @15:50:23.46 CSV.dial :flush @15:50:24.25 CS.seek 5.000ml @15:50:36.18 delay 5 seconds @15:50:41.19 CSV.dial :air @15:50:41.74 CS.seek 6.999ml @15:50:47.02 CSV.dial CTV @15:50:47.04
CTV.dial CSV @15:50:47.49 CBV.dial :waste2b @15:50:48.22 CS.seek 0.000ml @15:51:04.49 Disinfecting external Intake with kill @15:51:04.58 CSV.dial CSR @15:51:05.01 CSR.select :kill @15:51:05.11 CS.seek 8.000ml @15:51:23.74 delay 5 seconds @15:51:28.75 CSR.select :air @15:51:28.84 CS.seek 10.000ml @15:51:34.11 CSV.dial CBV @15:51:34.13
CBV.dial CSV @15:51:35.36 CTV.dial :intake @15:51:36.07 Intake.select :open @15:51:36.16 CS.seek 2.000ml @15:51:55.02 Intake.select :close @15:51:55.11 CTV.dial :air @15:51:55.82 CS.reconfigure CSconfig @15:51:55.86 CS.seek 0.000ml @15:52:01.77 CSV.dial CSR @15:52:02.74 CSR.select :flush @15:52:02.84 CS.seek 5.000ml @15:52:16.27 delay 5 seconds @15:52:16.58 delay 5 seconds @15:52:21.28
CSR.select :air @15:52:21.37 CS.seek 10.000ml @15:52:21.59 ATV.dial ARV @15:52:22.09 ASV.dial :PCRlock @15:52:22.65 delay 5 seconds @15:52:27.65 Running HF183em ProfileEM60C2 thermocycling @15:52:27.66 PCR.program ProfileEM60C2 @15:52:27.77 ProfileEM60C2 should complete in < 2:11:38 @15:52:27.78 PCR.run 2:49:32.5 @15:52:34.86
CSV.dial CBV @15:52:36.47 CS.seek 0.000ml @15:53:02.64 Garaging FlushPuck in Collection Clamp @15:53:02.70 Elbow.reconfigure TightElbow @15:53:02.73 Elbow.seek CC @15:53:02.83 Elbow.reconfigure LooseElbow @15:53:02.91 Forearm.seek CC @15:53:03.89 CC.seek sealed @15:53:04.83 Hand.grip @15:53:05.58 CC.seek open @15:53:08.89 Forearm.seek retract @15:53:09.84 Elbow.reconfigure TightElbow @15:53:09.87 Elbow.seek garage @15:53:12.60 Elbow.reconfigure LooseElbow @15:53:12.68 Forearm.reconfigure TightForearm @15:53:12.71 Forearm.seek garage @15:53:13.86 Hand.release @15:53:14.61 Forearm.reconfigure LooseForearm @15:53:14.64 Forearm.seek retract @17:48:57.82 PCR.off @17:48:58.35 PCR for HF183em completed -- closed /var/log/esp/HF183em14mar1415h499ml.pcr @17:48:58.40 Cleaning PCR AFV.select :carrierSolution @17:48:58.50 delay 2 seconds @17:49:00.51 AS.reconfigure ASconfig @17:49:00.54 AS.seek 0.500ml @17:49:10.96 delay 1 second @17:49:11.97 AFV.select :holdingCoil @17:49:12.06 ASV.dial :waste1 @17:49:12.51 ATV.dial :air3 @17:49:13.45 AS.reconfigure ASslow1 @17:49:13.48 AS.seek 0.510ml @17:49:15.77 delay 2 seconds @17:49:17.78 ATV.dial :bleach @17:49:18.70 AS.seek 0.810ml @17:50:20.15 delay 3 seconds @17:50:23.16 ATV.dial :air3 @17:50:23.50 delay 3 seconds @17:50:26.56 AS.seek 0.835ml @17:50:31.89 delay 3 seconds @17:50:34.90 ATV.dial :bleach @17:50:35.38 AS.seek 0.820ml @17:50:38.68 delay 3 seconds @17:50:41.69 ARV.dial :waste @17:50:42.29 delay 3 seconds @17:50:45.30 ATV.dial :ARV @17:50:46.05 AS.reconfigure ASconfig @17:50:46.08 AS.seek 0.470ml @17:51:06.75 AFV.select :carrierSolution @17:51:06.85 delay 2 seconds @17:51:08.86 AS.seek 1.000ml @17:51:40.08 delay 1 second @17:51:41.09 AFV.select :holdingCoil @17:51:41.18 delay 1 second @17:51:42.19 AS.reconfigure ASfast @17:51:42.23 AS.seek 0.000ml @17:52:09.97 AFV.select :carrierSolution @17:52:10.07 delay 2 seconds @17:52:12.08 AS.reconfigure ASconfig @17:52:12.11 AS.seek 1.000ml @17:53:10.70 delay 1 second @17:53:11.71 AFV.select :holdingCoil @17:53:11.80 delay 1 second @17:53:12.81 AS.reconfigure ASfast @17:53:12.84 AS.seek 0.000ml @17:53:40.59 AFV.select :carrierSolution @17:53:40.74 delay 2 seconds @17:53:42.75 AS.reconfigure ASconfig @17:53:42.78 AS.seek 1.000ml @17:54:41.38 delay 1 second @17:54:42.39 AFV.select :holdingCoil @17:54:42.48 delay 1 second @17:54:43.49 AS.reconfigure ASfast @17:54:43.52 AS.seek 0.000ml @17:55:11.26 ARV.dial between carrier and air1 @17:55:11.77 ATV.dial :air3 @17:55:12.79 AS.reconfigure ASslow1 @17:55:12.82 AS.seek 0.010ml @17:55:15.15 delay 2 seconds @17:55:17.16 ATV.dial :bleach @17:55:17.65 AS.seek 0.810ml @17:58:01.01 delay 3 seconds @17:58:04.02 ATV.dial :air3 @17:58:04.34 delay 3 seconds @17:58:07.40 AS.seek 0.835ml @17:58:12.73 delay 3 seconds @17:58:15.74 ATV.dial :bleach @17:58:16.13 AS.seek 0.820ml @17:58:19.44 delay 3 seconds @17:58:22.45 ATV.dial PCR @17:58:22.77 AS.reconfigure ASconfig @17:58:22.80 AS.seek empty @17:59:10.92 PCR.program :bleachProfile @17:59:10.98 PCR program should complete in < 2:45 @17:59:10.99 PCR.run 10:00 @18:02:09.18 PCR.off @18:02:09.21 AFV.select :carrierSolution @18:02:09.30 delay 2 seconds @18:02:11.31 AS.seek 1.000ml @18:03:09.96 delay 1 second @18:03:10.97 AFV.select :holdingCoil @18:03:11.06 delay 1 second @18:03:12.07 AS.reconfigure ASfast @18:03:12.10 AS.seek 0.000ml @18:03:39.82 delay 3 seconds @18:03:42.83 AFV.select :carrierSolution @18:03:42.92 delay 2 seconds @18:03:44.93 AS.reconfigure ASconfig @18:03:44.96 AS.seek 1.000ml @18:04:43.55 delay 1 second @18:04:44.56 AFV.select :holdingCoil @18:04:44.65 delay 1 second @18:04:45.66 AS.reconfigure ASfast @18:04:45.70 AS.seek 0.000ml @18:05:13.45 delay 3 seconds @18:05:16.46 AFV.select :carrierSolution @18:05:16.55 delay 2 seconds @18:05:18.56 AS.reconfigure ASconfig @18:05:18.59 AS.seek 1.000ml @18:06:17.19 delay 1 second @18:06:18.20 AFV.select :holdingCoil @18:06:18.29 delay 1 second @18:06:19.30 AS.reconfigure ASfast @18:06:19.33 AS.seek 0.000ml @18:06:47.06 delay 3 seconds @18:06:50.07 AFV.select :carrierSolution @18:06:50.16 delay 2 seconds @18:06:52.17 AS.reconfigure ASconfig @18:06:52.20 AS.seek 1.000ml @18:07:50.79 delay 1 second @18:07:51.80 AFV.select :holdingCoil @18:07:51.89 delay 1 second @18:07:52.90 AS.reconfigure ASfast @18:07:52.93 AS.seek 0.000ml @18:08:20.68 delay 3 seconds @18:08:23.69 AFV.select :carrierSolution @18:08:23.78 delay 2 seconds @18:08:25.79 AS.reconfigure ASconfig @18:08:25.82 AS.seek 1.000ml @18:09:24.40 delay 1 second @18:09:25.41 AFV.select :holdingCoil @18:09:25.50 delay 1 second @18:09:26.51 AS.reconfigure ASnorm @18:09:26.54 AS.seek 0.000ml @18:10:17.99 Setting up PCR for ENTap -- writing /var/log/esp/ENTap14mar1418h499ml.pcr @18:10:18.00 Building PCR Slug from ASV with MM1 mix, PP1 primer @18:10:18.01 Priming with carrier @18:10:18.02 ATV.dial ARV @18:10:18.54 ARV.dial :carrier @18:10:18.81 AS.seek 0.500ml @18:10:44.71 AFV.select :carrierSolution @18:10:44.80 delay 2 seconds @18:10:46.81 AS.reconfigure ASconfig @18:10:46.85 AS.seek 1.000ml @18:11:16.27 delay 1 second @18:11:17.28 AFV.select :holdingCoil @18:11:17.37 delay 1 second @18:11:18.38 ATV.dial :waste @18:11:19.65 AS.reconfigure ASnorm @18:11:19.68 AS.seek empty @18:12:11.15 AFV.select :carrierSolution @18:12:11.24 delay 2 seconds @18:12:13.25 AS.reconfigure ASconfig @18:12:13.28 AS.seek 0.500ml @18:12:43.26 delay 1 second @18:12:44.27 AFV.select :holdingCoil @18:12:44.36 Building Slug @18:12:44.37 ATV.dial :ARV @18:12:45.62 delay 2 seconds @18:12:47.63 ARV.dial :air1 @18:12:47.96 delay 2 seconds @18:12:50.02 AS.reconfigure ASslow2 @18:12:50.05 AS.seek 0.506ml @18:12:56.54 delay 2 seconds @18:12:58.55 ARV.dial :MM1 @18:12:59.12 delay 2 seconds @18:13:01.13 Adding MM1 @18:13:01.19 AS.seek 0.524ml @18:13:19.76 delay 25 seconds @18:13:44.77 ARV.dial :PP1 @18:13:45.10 Adding PP1 @18:13:45.11 delay 2 seconds @18:13:47.16 AS.seek 0.530ml @18:13:53.42 delay 10 seconds @18:14:03.43 ARV.dial :air1 @18:14:03.75 adding ARV air @18:14:03.76 delay 2 seconds @18:14:05.81 AS.seek 0.536ml @18:14:12.34 delay 5 seconds @18:14:17.35 ARV.dial :carrier @18:14:17.66 delay 7 seconds @18:14:24.72 AS.seek 0.594ml @18:15:24.35 delay 5 seconds @18:15:29.36 ATV.dial :ASV @18:15:29.69 delay 2 seconds @18:15:31.75 AS.seek 0.600ml @18:15:38.24 delay 4 seconds @18:15:42.25 ATV.dial ARV @18:15:42.58 delay 2 seconds @18:15:44.59 ARV.dial :carrier @18:15:44.60 delay 2 seconds @18:15:46.65 AS.seek 0.650ml @18:16:37.99 delay 4 seconds @18:16:42.00 ATV.dial PCR @18:16:42.50 ASV.dial :waste1 @18:16:42.51 ARV.dial :air1 @18:16:42.86 Positioning Slug by 0.324ml @18:16:42.91 AS.seek 0.326ml @18:22:13.51 delay 5 seconds @18:22:18.52 ATV.dial ARV @18:22:19.04 ASV.dial :PCRlock @18:22:19.52 delay 5 seconds @18:22:24.53 Running ENTap Profile60C thermocycling @18:22:24.54 PCR.program Profile60C @18:22:24.64 Profile60C should complete in < 1:39:08 @18:22:24.65 PCR.run 2:08:55 @19:45:19.49 PCR.off @19:45:20.49 PCR for ENTap completed -- closed /var/log/esp/ENTap14mar1418h499ml.pcr @19:45:20.54 Cleaning PCR @19:45:20.55 AFV.select :carrierSolution @19:45:20.64 delay 2 seconds @19:45:22.65 AS.reconfigure ASconfig @19:45:22.69 AS.seek 0.500ml @19:45:33.11 delay 1 second @19:45:34.12 AFV.select :holdingCoil @19:45:34.21 ASV.dial :waste1 @19:45:34.69 ATV.dial :air3 @19:45:35.63 AS.reconfigure ASslow1 @19:45:35.66 AS.seek 0.510ml @19:45:37.96 delay 2 seconds @19:45:39.97 ATV.dial :bleach @19:45:40.35 AS.seek 0.810ml @19:46:41.77 delay 3 seconds @19:46:44.78 ATV.dial :air3 @19:46:45.13 delay 3 seconds @19:46:48.19 AS.seek 0.835ml @19:46:53.53 delay 3 seconds @19:46:56.54 ATV.dial :bleach @19:46:56.93 AS.seek 0.820ml @19:47:00.23 delay 3 seconds @19:47:03.24 ARV.dial :waste @19:47:03.84 delay 3 seconds @19:47:06.85 ATV.dial :ARV @19:47:07.57 AS.reconfigure ASconfig @19:47:07.61 AS.seek 0.470ml @19:47:28.28 AFV.select :carrierSolution @19:47:28.37 delay 2 seconds @19:47:30.38 AS.seek 1.000ml @19:48:01.62 delay 1 second @19:48:02.63 AFV.select :holdingCoil @19:48:02.72 delay 1 second @19:48:03.73 AS.reconfigure ASfast @19:48:03.77 AS.seek 0.000ml @19:48:31.51 AFV.select :carrierSolution @19:48:31.61 delay 2 seconds @19:48:33.62 AS.reconfigure ASconfig @19:48:33.65 AS.seek 1.000ml @19:49:32.25 delay 1 second @19:49:33.26 AFV.select :holdingCoil @19:49:33.35 delay 1 second @19:49:34.36 AS.reconfigure ASfast @19:49:34.39 AS.seek 0.000ml @19:50:02.10 AFV.select :carrierSolution @19:50:02.20 delay 2 seconds @19:50:04.21 AS.reconfigure ASconfig @19:50:04.25 AS.seek 1.000ml @19:51:02.82 delay 1 second @19:51:03.83 AFV.select :holdingCoil @19:51:03.92 delay 1 second @19:51:04.93 AS.reconfigure ASfast @19:51:04.97 AS.seek 0.000ml @19:51:32.68 ARV.dial between carrier and air1 @19:51:33.09 ATV.dial :air3 @19:51:34.04 AS.reconfigure ASslow1 @19:51:34.07 AS.seek 0.010ml @19:51:36.40 delay 2 seconds @19:51:38.41 ATV.dial :bleach @19:51:38.81 AS.seek 0.810ml @19:54:22.23 delay 3 seconds @19:54:25.24 ATV.dial :air3 @19:54:25.59 delay 3 seconds @19:54:28.65 AS.seek 0.835ml @19:54:33.98 delay 3 seconds @19:54:36.99 ATV.dial :bleach @19:54:37.48 AS.seek 0.820ml @19:54:40.84 delay 3 seconds @19:54:43.85 ATV.dial PCR @19:54:44.30 AS.reconfigure ASconfig @19:54:44.33 AS.seek empty @19:55:32.46 PCR.program :bleachProfile @19:55:32.51 PCR program should complete in < 2:45 @19:55:32.52 PCR.run 10:00 @19:58:30.71 PCR.off @19:58:30.74 AFV.select :carrierSolution @19:58:30.83 delay 2 seconds @19:58:32.84 AS.seek 1.000ml @19:59:31.49 delay 1 second @19:59:32.50 AFV.select :holdingCoil @19:59:32.59 delay 1 second @19:59:33.60 AS.reconfigure ASfast @19:59:33.63 AS.seek 0.000ml @20:00:01.36 delay 3 seconds @20:00:04.37 AFV.select :carrierSolution @20:00:04.46 delay 2 seconds @20:00:06.47 AS.reconfigure ASconfig @20:00:06.50 AS.seek 1.000ml @20:01:05.08 delay 1 second @20:01:06.09 AFV.select :holdingCoil @20:01:06.29 delay 1 second @20:01:07.30 AS.reconfigure ASfast @20:01:07.33 AS.seek 0.000ml @20:01:35.00 delay 3 seconds @20:01:38.01 AFV.select :carrierSolution @20:01:38.10 delay 2 seconds @20:01:40.11 AS.reconfigure ASconfig @20:01:40.14 AS.seek 1.000ml @20:02:38.68 delay 1 second @20:02:39.69 AFV.select :holdingCoil @20:02:39.78 delay 1 second @20:02:40.79 AS.reconfigure ASfast @20:02:40.82 AS.seek 0.000ml @20:03:08.56 delay 3 seconds @20:03:11.57 AFV.select :carrierSolution @20:03:11.66 delay 2 seconds @20:03:13.67 AS.reconfigure ASconfig @20:03:13.70 AS.seek 1.000ml @20:04:12.29 delay 1 second @20:04:13.30 AFV.select :holdingCoil @20:04:13.39 delay 1 second @20:04:14.40 AS.reconfigure ASfast @20:04:14.43 AS.seek 0.000ml @20:04:42.16 delay 3 seconds @20:04:45.17 AFV.select :carrierSolution @20:04:45.26 delay 2 seconds @20:04:47.27 AS.reconfigure ASconfig @20:04:47.30 AS.seek 1.000ml @20:05:45.91 delay 1 second @20:05:46.92 AFV.select :holdingCoil @20:05:47.01 delay 1 second @20:05:48.02 AS.reconfigure ASnorm @20:05:48.05 AS.seek 0.000ml @20:06:39.52 Setting up PCR for ENTem -- writing /var/log/esp/ENTem14mar1420h499ml.pcr @20:06:39.53 Building PCR Slug from ASV with MM2 mix, PP3 primer @20:06:39.54 Priming with carrier @20:06:39.55 ATV.dial ARV @20:06:40.16 ARV.dial :carrier @20:06:40.44 AS.seek 0.500ml @20:07:06.31 AFV.select :carrierSolution @20:07:06.41 delay 2 seconds @20:07:08.42 AS.reconfigure ASconfig @20:07:08.46 AS.seek 1.000ml @20:07:37.89 delay 1 second @20:07:38.90 AFV.select :holdingCoil @20:07:39.00 delay 1 second @20:07:40.01 ATV.dial :waste @20:07:41.26 AS.reconfigure ASnorm @20:07:41.29 AS.seek empty @20:08:32.77 AFV.select :carrierSolution @20:08:32.88 delay 2 seconds @20:08:34.89 AS.reconfigure ASconfig @20:08:34.92 AS.seek 0.500ml @20:09:04.40 delay 1 second @20:09:05.41 AFV.select :holdingCoil @20:09:05.50 Building Slug @20:09:05.51 ATV.dial :ARV @20:09:06.76 delay 2 seconds @20:09:08.77 ARV.dial :air1 @20:09:09.09 delay 2 seconds @20:09:11.15 AS.reconfigure ASslow2 @20:09:11.18 AS.seek 0.506ml @20:09:17.58 delay 2 seconds @20:09:19.59 ARV.dial :MM2 @20:09:20.81 delay 2 seconds @20:09:22.82 Adding MM2 @20:09:22.87 AS.seek 0.524ml @20:09:41.53 delay 25 seconds @20:10:06.54 ARV.dial :PP3 @20:10:06.87 Adding PP3 @20:10:06.88 delay 2 seconds @20:10:08.94 AS.seek 0.530ml @20:10:15.31 delay 10 seconds @20:10:25.32 ARV.dial :air2 @20:10:25.76 adding ARV air @20:10:25.77 delay 2 seconds @20:10:27.82 AS.seek 0.536ml @20:10:34.39 delay 5 seconds @20:10:39.40 ARV.dial :carrier @20:10:40.46 delay 7 seconds @20:10:47.52 AS.seek 0.594ml @20:11:46.83 delay 5 seconds @20:11:51.84 ATV.dial :ASV @20:11:52.16 delay 2 seconds @20:11:54.22 AS.seek 0.600ml @20:12:00.55 delay 4 seconds @20:12:04.56 ATV.dial ARV @20:12:04.89 delay 2 seconds @20:12:06.90 ARV.dial :carrier @20:12:06.91 delay 2 seconds @20:12:08.96 AS.seek 0.650ml @20:13:00.09 delay 4 seconds @20:13:04.10 ATV.dial PCR @20:13:04.59 ASV.dial :waste1 @20:13:04.60 ARV.dial :air1 @20:13:04.94 Positioning Slug by 0.324ml @20:13:05.00 AS.seek 0.326ml @20:18:35.82 delay 5 seconds @20:18:40.83 ATV.dial ARV @20:18:41.43 ASV.dial :PCRlock @20:18:41.91 delay 5 seconds @20:18:46.92 Running ENTem ProfileEM60C thermocycling @20:18:46.93 PCR.program ProfileEM60C @20:18:47.04 ProfileEM60C should complete in < 2:11:38 @20:18:47.05 PCR.run 2:49:32.5 @22:14:14.11 PCR.off @22:14:14.65 PCR for ENTem completed -- closed /var/log/esp/ENTem14mar1420h499ml.pcr @22:14:14.70 Cleaning PCR @22:14:14.71 AFV.select :carrierSolution @22:14:14.80 delay 2 seconds @22:14:16.81 AS.reconfigure ASconfig @22:14:16.85 AS.seek 0.500ml @22:14:27.26 delay 1 second @22:14:28.27 AFV.select :holdingCoil @22:14:28.36 ASV.dial :waste1 @22:14:28.85 ATV.dial :air3 @22:14:29.79 AS.reconfigure ASslow1 @22:14:29.82 AS.seek 0.510ml @22:14:32.15 delay 2 seconds @22:14:34.16 ATV.dial :bleach @22:14:34.54 AS.seek 0.810ml @22:15:35.96 delay 3 seconds @22:15:38.97 ATV.dial :air3 @22:15:39.32 delay 3 seconds @22:15:42.38 AS.seek 0.835ml @22:15:47.71 delay 3 seconds @22:15:50.72 ATV.dial :bleach @22:15:51.12 AS.seek 0.820ml @22:15:54.44 delay 3 seconds @22:15:57.45 ARV.dial :waste @22:15:58.04 delay 3 seconds @22:16:01.05 ATV.dial :ARV @22:16:01.89 AS.reconfigure ASconfig @22:16:01.93 AS.seek 0.470ml @22:16:22.61 AFV.select :carrierSolution @22:16:22.71 delay 2 seconds @22:16:24.72 AS.seek 1.000ml @22:16:55.91 delay 1 second @22:16:56.92 AFV.select :holdingCoil @22:16:57.01 delay 1 second @22:16:58.02 AS.reconfigure ASfast @22:16:58.05 AS.seek 0.000ml @22:17:25.78 AFV.select :carrierSolution @22:17:25.88 delay 2 seconds @22:17:27.89 AS.reconfigure ASconfig @22:17:27.92 AS.seek 1.000ml @22:18:26.52 delay 1 second @22:18:27.53 AFV.select :holdingCoil @22:18:27.62 delay 1 second @22:18:28.63 AS.reconfigure ASfast @22:18:28.66 AS.seek 0.000ml @22:18:56.41 AFV.select :carrierSolution @22:18:56.51 delay 2 seconds @22:18:58.52 AS.reconfigure ASconfig @22:18:58.55 AS.seek 1.000ml @22:19:57.14 delay 1 second @22:19:58.15 AFV.select :holdingCoil @22:19:58.24 delay 1 second @22:19:59.25 AS.reconfigure ASfast @22:19:59.28 AS.seek 0.000ml @22:20:27.01 ARV.dial between carrier and air1 @22:20:27.42 ATV.dial :air3 @22:20:28.33 AS.reconfigure ASslow1 @22:20:28.37 AS.seek 0.010ml @22:20:30.70 delay 2 seconds @22:20:32.71 ATV.dial :bleach @22:20:33.65 AS.seek 0.810ml @22:23:17.04 delay 3 seconds @22:23:20.05 ATV.dial :air3 @22:23:20.38 delay 3 seconds @22:23:23.44 AS.seek 0.835ml @22:23:28.74 delay 3 seconds @22:23:31.75 ATV.dial :bleach @22:23:32.14 AS.seek 0.820ml @22:23:35.49 delay 3 seconds @22:23:38.50 ATV.dial PCR @22:23:38.81 AS.reconfigure ASconfig @22:23:38.84 AS.seek empty @22:24:26.96 PCR.program :bleachProfile @22:24:27.01 PCR program should complete in < 2:45 @22:24:27.02 PCR.run 10:00 @22:27:28.24 PCR.off @22:27:28.27 AFV.select :carrierSolution @22:27:28.36 delay 2 seconds @22:27:30.37 AS.seek 1.000ml @22:28:29.02 delay 1 second @22:28:30.03 AFV.select :holdingCoil @22:28:30.12 delay 1 second @22:28:31.13 AS.reconfigure ASfast @22:28:31.32 AS.seek 0.000ml @22:28:59.05 delay 3 seconds @22:29:02.06 AFV.select :carrierSolution @22:29:02.15 delay 2 seconds @22:29:04.16 AS.reconfigure ASconfig @22:29:04.19 AS.seek 1.000ml @22:30:02.80 delay 1 second @22:30:03.81 AFV.select :holdingCoil @22:30:03.90 delay 1 second @22:30:04.91 AS.reconfigure ASfast @22:30:04.95 AS.seek 0.000ml @22:30:32.69 delay 3 seconds @22:30:35.70 AFV.select :carrierSolution @22:30:35.79 delay 2 seconds @22:30:37.80 AS.reconfigure ASconfig @22:30:37.83 AS.seek 1.000ml @22:31:36.42 delay 1 second @22:31:37.43 AFV.select :holdingCoil @22:31:37.52 delay 1 second @22:31:38.53 AS.reconfigure ASfast @22:31:38.56 AS.seek 0.000ml @22:32:06.30 delay 3 seconds @22:32:09.31 AFV.select :carrierSolution @22:32:09.40 delay 2 seconds @22:32:11.41 AS.reconfigure ASconfig @22:32:11.96 AS.seek 1.000ml @22:33:10.56 delay 1 second @22:33:11.57 AFV.select :holdingCoil @22:33:11.66 delay 1 second @22:33:12.67 AS.reconfigure ASfast @22:33:12.70 AS.seek 0.000ml @22:33:40.42 delay 3 seconds @22:33:43.43 AFV.select :carrierSolution @22:33:43.52 delay 2 seconds @22:33:45.53 AS.reconfigure ASconfig @22:33:45.56 AS.seek 1.000ml @22:34:44.18 delay 1 second @22:34:45.19 AFV.select :holdingCoil @22:34:45.28 delay 1 second @22:34:46.29 AS.reconfigure ASnorm @22:34:46.33 AS.seek 0.000ml @22:35:37.79 Setting up PCR for IPCap -- writing /var/log/esp/IPCap14mar1422h499ml.pcr @22:35:37.80 Building PCR Slug from ASV with MM1 mix, PP2 primer @22:35:37.81 Priming with carrier @22:35:37.82 ATV.dial ARV @22:35:38.34 ARV.dial :carrier @22:35:38.62 AS.seek 0.500ml @22:36:04.49 AFV.select :carrierSolution @22:36:04.59 delay 2 seconds @22:36:06.60 AS.reconfigure ASconfig @22:36:06.64 AS.seek 1.000ml @22:36:36.05 delay 1 second @22:36:37.06 AFV.select :holdingCoil @22:36:37.16 delay 1 second @22:36:38.17 ATV.dial :waste @22:36:39.42 AS.reconfigure ASnorm @22:36:39.45 AS.seek empty @22:37:30.91 AFV.select :carrierSolution @22:37:31.01 delay 2 seconds @22:37:33.02 AS.reconfigure ASconfig @22:37:33.05 AS.seek 0.500ml @22:38:02.54 delay 1 second @22:38:03.55 AFV.select :holdingCoil @22:38:03.64 Building Slug @22:38:03.65 ATV.dial :ARV @22:38:04.90 delay 2 seconds @22:38:06.91 ARV.dial :air1 @22:38:07.24 delay 2 seconds @22:38:09.30 AS.reconfigure ASslow2 @22:38:09.33 AS.seek 0.506ml @22:38:15.68 delay 2 seconds @22:38:17.69 ARV.dial :MM1 @22:38:18.19 delay 2 seconds @22:38:20.20 Adding MM1 @22:38:20.25 AS.seek 0.524ml @22:38:38.94 delay 25 seconds @22:39:03.95 ARV.dial :PP2 @22:39:04.38 Adding PP2 @22:39:04.40 delay 2 seconds @22:39:06.45 AS.seek 0.530ml @22:39:12.88 delay 10 seconds @22:39:22.89 ARV.dial :air2 @22:39:23.22 adding ARV air @22:39:23.23 delay 2 seconds @22:39:25.29 AS.seek 0.536ml @22:39:31.63 delay 5 seconds @22:39:36.64 ARV.dial :carrier @22:39:37.67 delay 7 seconds @22:39:44.73 AS.seek 0.594ml @22:40:44.06 delay 5 seconds @22:40:49.07 ATV.dial :ASV @22:40:49.48 delay 2 seconds @22:40:51.54 AS.seek 0.600ml @22:40:57.78 delay 4 seconds @22:41:01.79 ATV.dial ARV @22:41:02.11 delay 2 seconds @22:41:04.12 ARV.dial :carrier @22:41:04.13 delay 2 seconds @22:41:06.18 AS.seek 0.650ml @22:41:57.34 delay 4 seconds @22:42:01.35 ATV.dial PCR @22:42:01.86 ASV.dial :waste1 @22:42:01.87 ARV.dial :air1 @22:42:02.21 Positioning Slug by 0.324ml @22:42:02.26 AS.seek 0.326ml @22:47:32.78 delay 5 seconds @22:47:37.79 ATV.dial ARV @22:47:38.40 ASV.dial :PCRlock @22:47:38.89 delay 5 seconds @22:47:43.90 Running IPCap Profile59C thermocycling @22:47:43.91 PCR.program Profile59C @22:47:44.01 Profile59C should complete in < 1:39:08 @22:47:44.02 PCR.run 2:08:55 @00:10:41.36PDT15-Mar-14 PCR.off @00:10:41.90 PCR for IPCap completed -- closed /var/log/esp/IPCap14mar1422h499ml.pcr @00:10:41.95 Cleaning PCR @00:10:41.96 AFV.select :carrierSolution @00:10:42.05 delay 2 seconds @00:10:44.06 AS.reconfigure ASconfig @00:10:44.09 AS.seek 0.500ml @00:10:54.51 delay 1 second @00:10:55.52 AFV.select :holdingCoil @00:10:55.61 ASV.dial :waste1 @00:10:56.09 ATV.dial :air3 @00:10:57.02 AS.reconfigure ASslow1 @00:10:57.06 AS.seek 0.510ml @00:10:59.37 delay 2 seconds @00:11:01.38 ATV.dial :bleach @00:11:01.75 AS.seek 0.810ml @00:12:03.17 delay 3 seconds @00:12:06.18 ATV.dial :air3 @00:12:06.51 delay 3 seconds @00:12:09.57 AS.seek 0.835ml @00:12:14.92 delay 3 seconds @00:12:17.93 ATV.dial :bleach @00:12:18.33 AS.seek 0.820ml @00:12:21.64 delay 3 seconds @00:12:24.65 ARV.dial :waste @00:12:25.25 delay 3 seconds @00:12:28.26 ATV.dial :ARV @00:12:28.99 AS.reconfigure ASconfig @00:12:29.03 AS.seek 0.470ml @00:12:49.70 AFV.select :carrierSolution @00:12:49.79 delay 2 seconds @00:12:51.80 AS.seek 1.000ml @00:13:23.01 delay 1 second @00:13:24.02 AFV.select :holdingCoil @00:13:24.11 delay 1 second @00:13:25.12 AS.reconfigure ASfast @00:13:25.15 AS.seek 0.000ml @00:13:52.87 AFV.select :carrierSolution @00:13:52.96 delay 2 seconds @00:13:54.97 AS.reconfigure ASconfig @00:13:55.00 AS.seek 1.000ml @00:14:53.55 delay 1 second @00:14:54.56 AFV.select :holdingCoil @00:14:54.65 delay 1 second @00:14:55.66 AS.reconfigure ASfast @00:14:55.69 AS.seek 0.000ml @00:15:23.43 AFV.select :carrierSolution @00:15:23.53 delay 2 seconds @00:15:25.54 AS.reconfigure ASconfig @00:15:25.57 AS.seek 1.000ml @00:16:24.13 delay 1 second @00:16:25.14 AFV.select :holdingCoil @00:16:25.23 delay 1 second @00:16:26.24 AS.reconfigure ASfast @00:16:26.39 AS.seek 0.000ml @00:16:54.10 ARV.dial between carrier and air1 @00:16:54.63 ATV.dial :air3 @00:16:55.57 AS.reconfigure ASslow1 @00:16:55.61 AS.seek 0.010ml @00:16:57.97 delay 2 seconds @00:16:59.98 ATV.dial :bleach @00:17:00.38 AS.seek 0.810ml @00:19:43.76 delay 3 seconds @00:19:46.77 ATV.dial :air3 @00:19:47.11 delay 3 seconds @00:19:50.17 AS.seek 0.835ml @00:19:55.53 delay 3 seconds @00:19:58.54 ATV.dial :bleach @00:19:58.94 AS.seek 0.820ml @00:20:02.29 delay 3 seconds @00:20:05.30 ATV.dial PCR @00:20:05.61 AS.reconfigure ASconfig @00:20:05.65 AS.seek empty @00:20:53.78 PCR.program :bleachProfile @00:20:53.83 PCR program should complete in < 2:45 @00:20:53.84 PCR.run 10:00 @00:23:55.07 PCR.off @00:23:55.10 AFV.select :carrierSolution @00:23:55.19 delay 2 seconds @00:23:57.20 AS.seek 1.000ml @00:24:55.88 delay 1 second @00:24:56.89 AFV.select :holdingCoil @00:24:56.98 delay 1 second @00:24:57.99 AS.reconfigure ASfast @00:24:58.02 AS.seek 0.000ml @00:25:26.30 delay 3 seconds @00:25:29.31 AFV.select :carrierSolution @00:25:29.40 delay 2 seconds @00:25:31.41 AS.reconfigure ASconfig @00:25:31.44 AS.seek 1.000ml @00:26:30.06 delay 1 second @00:26:31.07 AFV.select :holdingCoil @00:26:31.16 delay 1 second @00:26:32.17 AS.reconfigure ASfast @00:26:32.20 AS.seek 0.000ml @00:26:59.93 delay 3 seconds @00:27:02.94 AFV.select :carrierSolution @00:27:03.03 delay 2 seconds @00:27:05.04 AS.reconfigure ASconfig @00:27:05.07 AS.seek 1.000ml @00:28:03.66 delay 1 second @00:28:04.67 AFV.select :holdingCoil @00:28:04.76 delay 1 second @00:28:05.77 AS.reconfigure ASfast @00:28:05.80 AS.seek 0.000ml @00:28:33.54 delay 3 seconds @00:28:36.55 AFV.select :carrierSolution @00:28:36.64 delay 2 seconds @00:28:38.65 AS.reconfigure ASconfig @00:28:38.68 AS.seek 1.000ml @00:29:37.25 delay 1 second @00:29:38.26 AFV.select :holdingCoil @00:29:38.35 delay 1 second @00:29:39.36 AS.reconfigure ASfast @00:29:39.39 AS.seek 0.000ml @00:30:07.10 delay 3 seconds @00:30:10.11 AFV.select :carrierSolution @00:30:10.20 delay 2 seconds @00:30:12.21 AS.reconfigure ASconfig @00:30:12.24 AS.seek 1.000ml @00:31:10.83 delay 1 second @00:31:11.84 AFV.select :holdingCoil @00:31:11.93 delay 1 second @00:31:12.94 AS.reconfigure ASnorm @00:31:12.97 AS.seek 0.000ml @00:32:04.44 Setting up PCR for IPCem -- writing /var/log/esp/IPCem14mar1500h499ml.pcr @00:32:04.45 Building PCR Slug from ASV with MM3 mix, PP5 primer @00:32:04.46 Priming with carrier @00:32:04.47 ATV.dial ARV @00:32:04.98 ARV.dial :carrier @00:32:05.25 AS.seek 0.500ml @00:32:31.12 AFV.select :carrierSolution @00:32:31.22 delay 2 seconds @00:32:33.23 AS.reconfigure ASconfig @00:32:33.27 AS.seek 1.000ml @00:33:02.70 delay 1 second @00:33:03.71 AFV.select :holdingCoil @00:33:03.80 delay 1 second @00:33:04.81 ATV.dial :waste @00:33:06.16 AS.reconfigure ASnorm @00:33:06.19 AS.seek empty @00:33:57.67 AFV.select :carrierSolution @00:33:57.77 delay 2 seconds @00:33:59.78 AS.reconfigure ASconfig @00:33:59.81 AS.seek 0.500ml @00:34:29.29 delay 1 second @00:34:30.30 AFV.select :holdingCoil @00:34:30.39 Building Slug @00:34:30.40 ATV.dial :ARV @00:34:31.76 delay 2 seconds @00:34:33.77 ARV.dial :air1 @00:34:34.09 delay 2 seconds @00:34:36.15 AS.reconfigure ASslow2 @00:34:36.18 AS.seek 0.506ml @00:34:42.43 delay 2 seconds @00:34:44.44 ARV.dial :MM3 @00:34:45.40 delay 2 seconds @00:34:47.41 Adding MM3 @00:34:47.47 AS.seek 0.524ml @00:35:06.01 delay 25 seconds @00:35:31.02 ARV.dial :PP5 @00:35:31.36 Adding PP5 @00:35:31.37 delay 2 seconds @00:35:33.42 AS.seek 0.530ml @00:35:39.77 delay 10 seconds @00:35:49.78 ARV.dial :air3 @00:35:50.21 adding ARV air @00:35:50.22 delay 2 seconds @00:35:52.27 AS.seek 0.536ml @00:35:58.73 delay 5 seconds @00:36:03.74 ARV.dial :carrier @00:36:04.85 delay 7 seconds @00:36:11.91 AS.seek 0.594ml @00:37:11.30 delay 5 seconds @00:37:16.31 ATV.dial :ASV @00:37:16.65 delay 2 seconds @00:37:18.71 AS.seek 0.600ml @00:37:25.13 delay 4 seconds @00:37:29.14 ATV.dial ARV @00:37:29.47 delay 2 seconds @00:37:31.48 ARV.dial :carrier @00:37:31.49 delay 2 seconds @00:37:33.54 AS.seek 0.650ml @00:38:24.71 delay 4 seconds @00:38:28.72 ATV.dial PCR @00:38:29.24 ASV.dial :waste1 @00:38:29.25 ARV.dial :air1 @00:38:29.59 Positioning Slug by 0.324ml @00:38:29.64 AS.seek 0.326ml @00:44:00.27 delay 5 seconds @00:44:05.28 ATV.dial ARV @00:44:05.89 ASV.dial :PCRlock @00:44:06.38 delay 5 seconds @00:44:11.39 Running IPCem ProfileEM59C thermocycling @00:44:11.40 PCR.program ProfileEM59C @00:44:11.51 ProfileEM59C should complete in < 2:11:38 @00:44:11.52 PCR.run 2:49:32.5 @02:39:23.34 PCR.off @02:39:23.88 PCR for IPCem completed -- closed /var/log/esp/IPCem14mar1500h499ml.pcr @02:39:23.89 Full MFB Decontamination Decon extraction column and decon AIV @02:39:23.91 SPE.hold 2:46:40,55.0C @02:39:23.92 ASV.dial :SPEdelivery @02:39:24.88 delay 20 seconds @02:39:44.89 AFV.select :carrierSolution @02:39:44.98 delay 2 seconds @02:39:46.99 AS.reconfigure ASconfig @02:39:47.02 AS.seek 0.500ml @02:39:57.44 delay 1 second @02:39:58.45 AFV.select :holdingCoil @02:39:58.54 ATV.dial :air1 @02:40:00.07 AS.reconfigure ASslow1 @02:40:00.10 AS.seek 0.520ml @02:40:04.43 ATV.dial :ASV @02:40:05.74 ASV.dial :SPErecovery @02:40:06.73 AS.seek 0.620ml @02:40:27.38 ATV.dial :waste @02:40:28.84 AS.reconfigure ASnorm @02:40:28.87 AS.seek empty @02:41:00.89 AFV.select :holdingCoil @02:41:00.90 ATV.dial :waste @02:41:00.91 AS.reconfigure ASconfig @02:41:00.94 AS.seek 0.000ml @02:41:01.29 AFV.select :carrierSolution @02:41:01.38 delay 2 seconds @02:41:03.39 AS.seek 0.500ml @02:41:32.84 delay 1 second @02:41:33.85 AFV.select :holdingCoil @02:41:33.94 ATV.dial :air3 @02:41:34.50 AS.reconfigure ASslow1 @02:41:34.53 AS.seek 0.520ml @02:41:38.86 ATV.dial :air3 @02:41:38.92 AS.seek 0.530ml @02:41:41.20 delay 2 seconds @02:41:43.21 ATV.dial :bleach @02:41:43.58 AS.seek 0.710ml @02:42:20.47 delay 3 seconds @02:42:23.48 ATV.dial :air3 @02:42:23.81 delay 3 seconds @02:42:26.87 AS.seek 0.735ml @02:42:32.20 delay 3 seconds @02:42:35.21 ATV.dial :bleach @02:42:35.69 AS.seek 0.720ml @02:42:38.98 delay 3 seconds @02:42:41.99 ATV.dial ASV @02:42:42.98 ASV.dial :SPErecovery @02:42:43.04 AS.seek 0.365ml @02:43:55.68 ATV.dial :waste @02:43:57.16 AS.reconfigure ASnorm @02:43:57.19 AS.seek empty @02:44:16.22 ATV.dial :air3 @02:44:16.78 AS.reconfigure ASslow1 @02:44:16.82 AS.seek 0.009ml @02:44:19.18 delay 2 seconds @02:44:21.19 ATV.dial :bleach @02:44:21.57 AS.seek 0.579ml @02:46:18.08 delay 3 seconds @02:46:21.09 ATV.dial :air3 @02:46:21.45 delay 3 seconds @02:46:24.50 AS.seek 0.604ml @02:46:29.85 delay 3 seconds @02:46:32.86 ATV.dial :bleach @02:46:33.25 AS.seek 0.589ml @02:46:36.55 delay 3 seconds @02:46:39.56 ATV.dial :ASV @02:46:40.53 ASV.dial :wasteR @02:46:40.87 AS.seek 0.000ml @02:48:41.31 delay 1:00 @02:49:41.32 ATV.dial ASV @02:49:41.33 AFV.select :carrierSolution @02:49:41.42 delay 2 seconds @02:49:43.43 AS.reconfigure ASconfig @02:49:43.46 AS.seek 1.000ml @02:50:42.07 delay 1 second @02:50:43.08 AFV.select :holdingCoil @02:50:43.17 delay 1 second @02:50:44.18 ASV.dial :wasteR @02:50:44.19 AS.reconfigure ASslow1 @02:50:44.22 AS.seek 0.000ml @02:54:08.47 delay 10 seconds @02:54:18.48 ATV.dial :air3 @02:54:19.60 AS.seek 0.010ml @02:54:21.92 delay 2 seconds @02:54:23.93 ATV.dial :bleach @02:54:24.32 AS.seek 0.580ml @02:56:20.81 delay 3 seconds @02:56:23.82 ATV.dial :air3 @02:56:24.16 delay 3 seconds @02:56:27.22 AS.seek 0.605ml @02:56:32.58 delay 3 seconds @02:56:35.59 ATV.dial :bleach @02:56:35.99 AS.seek 0.590ml @02:56:39.30 delay 3 seconds @02:56:42.31 ATV.dial ASV @02:56:43.19 ASV.dial :SPEdelivery @02:56:43.52 AS.seek 0.000ml @02:58:44.06 delay 1:00 @02:59:44.07 AFV.select :carrierSolution @02:59:44.16 delay 2 seconds @02:59:46.17 AS.reconfigure ASconfig @02:59:46.21 AS.seek 1.000ml @03:00:44.83 delay 1 second @03:00:45.84 AFV.select :holdingCoil @03:00:45.93 delay 1 second @03:00:46.94 ATV.dial ASV @03:00:46.95 ASV.dial :SPEdelivery @03:00:46.96 AS.reconfigure ASslow1 @03:00:46.99 AS.seek 0.000ml @03:04:11.21 delay 10 seconds @03:04:21.22 ATV.dial :air2 @03:04:21.94 AS.reconfigure ASslow0 @03:04:21.97 AS.seek 0.800ml @03:05:43.85 delay 3 seconds @03:05:46.86 ATV.dial :ASV @03:05:47.57 ASV.dial :SPEdelivery @03:05:47.59 AS.reconfigure ASslow1 @03:05:47.62 AS.seek 0.030ml @03:08:24.91 delay 20 seconds @03:08:44.92 ATV.dial :waste,via => air3 @03:08:46.37 AS.reconfigure ASconfig @03:08:46.40 AS.seek empty @03:08:48.47 SPE.coast @03:08:48.55 Removing Bleach from Mixing Coil @03:08:48.56 AFV.select :carrierSolution @03:08:48.65 delay 2 seconds @03:08:50.66 AS.seek 0.500ml @03:09:20.14 delay 1 second @03:09:21.15 AFV.select :holdingCoil @03:09:21.24 ATV.dial :ASV @03:09:22.69 ASV.dial :SPErecovery @03:09:23.62 AS.reconfigure ASslow1 @03:09:23.66 AS.seek 0.730ml @03:10:10.83 ASV.dial :wasteD @03:10:11.47 AS.reconfigure ASnorm @03:10:11.50 AS.seek 0.000ml @03:10:49.11 AFV.select :carrierSolution @03:10:49.20 delay 2 seconds @03:10:51.21 AS.reconfigure ASconfig @03:10:51.24 AS.seek 0.500ml @03:11:20.68 delay 1 second @03:11:21.69 AFV.select :holdingCoil @03:11:21.78 ATV.dial ASV @03:11:21.79 ASV.dial :SPErecovery @03:11:22.50 AS.reconfigure ASslow0 @03:11:22.53 AS.seek 0.300ml @03:11:43.21 ATV.dial :air2 @03:11:43.96 AS.reconfigure ASslow1 @03:11:44.00 AS.seek 0.320ml @03:11:48.34 ATV.dial ASV @03:11:49.08 AS.reconfigure ASslow0 @03:11:49.12 AS.seek 0.570ml @03:12:14.93 ASV.dial :wasteD @03:12:15.60 AS.reconfigure ASnorm @03:12:15.63 AS.seek 0.000ml @03:12:45.10 AFV.select :carrierSolution @03:12:45.20 delay 2 seconds @03:12:47.21 AS.reconfigure ASconfig @03:12:47.24 AS.seek 0.500ml @03:13:16.70 delay 1 second @03:13:17.71 AFV.select :holdingCoil @03:13:17.80 ATV.dial ASV @03:13:17.81 ASV.dial :SPErecovery @03:13:18.50 AS.reconfigure ASslow0 @03:13:18.54 AS.seek 0.300ml @03:13:39.23 ATV.dial :air2 @03:13:40.09 AS.reconfigure ASslow1 @03:13:40.12 AS.seek 0.320ml @03:13:44.46 ATV.dial ASV @03:13:45.20 AS.reconfigure ASslow0 @03:13:45.24 AS.seek 0.570ml @03:14:10.98 ASV.dial :wasteD @03:14:11.92 AS.reconfigure ASnorm @03:14:11.95 AS.seek 0.000ml @03:14:41.41 ATV.dial :air2 @03:14:42.24 AS.reconfigure ASslow0 @03:14:42.27 AS.seek 0.300ml @03:15:13.24 ATV.dial :ASV @03:15:13.94 ASV.dial :wasteD @03:15:13.96 AS.reconfigure ASconfig @03:15:13.99 AS.seek 0.050ml @03:15:28.82 ATV.dial :waste @03:15:30.34 AS.seek 0.000ml @03:15:33.52 ASV.dial :SPEdelivery @03:15:34.36 deconLysateINT @03:15:34.37 ATV.dial :air3 @03:15:34.94 AS.reconfigure ASslow1 @03:15:34.98 AS.seek 0.010ml @03:15:37.32 delay 2 seconds @03:15:39.33 ATV.dial :bleach @03:15:39.80 AS.seek 0.960ml @03:18:53.81 delay 3 seconds @03:18:56.82 ATV.dial :air3 @03:18:57.15 delay 3 seconds @03:19:00.21 AS.seek 0.985ml @03:19:05.52 delay 3 seconds @03:19:08.53 ATV.dial :bleach @03:19:08.92 AS.seek 0.970ml @03:19:12.23 delay 3 seconds @03:19:15.24 ATV.dial :AIV @03:19:16.30 AIV.dial :coilToMFB @03:19:16.31 AWV.select :waste @03:19:16.32 AFV.select :carrierSolution @03:19:16.41 delay 2 seconds @03:19:18.42 AS.reconfigure ASconfig @03:19:18.46 AS.seek 1.000ml @03:19:20.52 delay 1 second @03:19:21.53 AFV.select :holdingCoil @03:19:21.62 delay 1 second @03:19:22.63 AS.reconfigure ASslow0 @03:19:22.66 AS.seek empty @03:21:05.08 AFV.select :carrierSolution @03:21:05.18 delay 2 seconds @03:21:07.19 AS.reconfigure ASconfig @03:21:07.23 AS.seek 1.000ml @03:22:05.88 delay 1 second @03:22:06.89 AFV.select :holdingCoil @03:22:06.98 delay 1 second @03:22:07.99 AS.reconfigure ASslow0 @03:22:08.02 AS.seek empty @03:23:50.42 AFV.select :carrierSolution @03:23:50.52 delay 2 seconds @03:23:52.53 AS.reconfigure ASconfig @03:23:52.57 AS.seek 1.000ml @03:24:51.20 delay 1 second @03:24:52.21 AFV.select :holdingCoil @03:24:52.30 delay 1 second @03:24:53.31 AS.reconfigure ASslow0 @03:24:53.34 AS.seek empty @03:26:35.74 ATV.dial :air2 @03:26:36.33 AS.reconfigure ASslow1 @03:26:36.36 AS.seek 0.009ml @03:26:38.71 AS.reconfigure ASslow0 @03:26:38.75 AS.seek 1.000ml @03:28:20.08 ATV.dial AIV @03:28:20.63 AS.seek 0.020ml @03:30:00.91 ATV.dial :waste @03:30:02.20 AS.seek 0.000ml @03:30:04.52 AWV.select :air @03:30:04.67 Cleaning PCR @03:30:04.68 AFV.select :carrierSolution @03:30:04.77 delay 2 seconds @03:30:06.78 AS.reconfigure ASconfig @03:30:06.81 AS.seek 0.500ml @03:30:36.25 delay 1 second @03:30:37.26 AFV.select :holdingCoil @03:30:37.35 ASV.dial :waste1 @03:30:37.67 ATV.dial :air3 @03:30:38.31 AS.reconfigure ASslow1 @03:30:38.35 AS.seek 0.510ml @03:30:40.67 delay 2 seconds @03:30:42.68 ATV.dial :bleach @03:30:43.05 AS.seek 0.810ml @03:31:44.47 delay 3 seconds @03:31:47.48 ATV.dial :air3 @03:31:47.81 delay 3 seconds @03:31:50.87 AS.seek 0.835ml @03:31:56.20 delay 3 seconds @03:31:59.21 ATV.dial :bleach @03:31:59.69 AS.seek 0.820ml @03:32:03.01 delay 3 seconds @03:32:06.02 ARV.dial :waste @03:32:06.53 delay 3 seconds @03:32:09.54 ATV.dial :ARV @03:32:10.29 AS.reconfigure ASconfig @03:32:10.33 AS.seek 0.470ml @03:32:30.98 AFV.select :carrierSolution @03:32:31.07 delay 2 seconds @03:32:33.08 AS.seek 1.000ml @03:33:04.29 delay 1 second @03:33:05.30 AFV.select :holdingCoil @03:33:05.39 delay 1 second @03:33:06.40 AS.reconfigure ASfast @03:33:06.43 AS.seek 0.000ml @03:33:34.18 AFV.select :carrierSolution @03:33:34.28 delay 2 seconds @03:33:36.29 AS.reconfigure ASconfig @03:33:36.32 AS.seek 1.000ml @03:34:34.94 delay 1 second @03:34:35.95 AFV.select :holdingCoil @03:34:36.04 delay 1 second @03:34:37.05 AS.reconfigure ASfast @03:34:37.08 AS.seek 0.000ml @03:35:04.83 AFV.select :carrierSolution @03:35:04.93 delay 2 seconds @03:35:06.94 AS.reconfigure ASconfig @03:35:06.97 AS.seek 1.000ml @03:36:05.58 delay 1 second @03:36:06.59 AFV.select :holdingCoil @03:36:06.68 delay 1 second @03:36:07.69 AS.reconfigure ASfast @03:36:07.72 AS.seek 0.000ml @03:36:35.45 ARV.dial between carrier and air1 @03:36:35.88 ATV.dial :air3 @03:36:36.92 AS.reconfigure ASslow1 @03:36:36.96 AS.seek 0.010ml @03:36:39.30 delay 2 seconds @03:36:41.31 ATV.dial :bleach @03:36:41.69 AS.seek 0.810ml @03:39:25.07 delay 3 seconds @03:39:28.08 ATV.dial :air3 @03:39:28.40 delay 3 seconds @03:39:31.46 AS.seek 0.835ml @03:39:36.81 delay 3 seconds @03:39:39.82 ATV.dial :bleach @03:39:40.22 AS.seek 0.820ml @03:39:43.54 delay 3 seconds @03:39:46.55 ATV.dial PCR @03:39:46.86 AS.reconfigure ASconfig @03:39:46.89 AS.seek empty @03:40:35.01 PCR.program :bleachProfile @03:40:35.07 PCR program should complete in < 2:45 @03:40:35.08 PCR.run 10:00 @03:43:42.81 PCR.off @03:43:42.84 AFV.select :carrierSolution @03:43:42.93 delay 2 seconds @03:43:44.94 AS.seek 1.000ml @03:44:43.61 delay 1 second @03:44:44.62 AFV.select :holdingCoil @03:44:44.71 delay 1 second @03:44:45.72 AS.reconfigure ASfast @03:44:45.75 AS.seek 0.000ml @03:45:13.50 delay 3 seconds @03:45:16.51 AFV.select :carrierSolution @03:45:16.60 delay 2 seconds @03:45:18.61 AS.reconfigure ASconfig @03:45:18.64 AS.seek 1.000ml @03:46:17.22 delay 1 second @03:46:18.23 AFV.select :holdingCoil @03:46:18.32 delay 1 second @03:46:19.33 AS.reconfigure ASfast @03:46:19.36 AS.seek 0.000ml @03:46:47.09 delay 3 seconds @03:46:50.10 AFV.select :carrierSolution @03:46:50.19 delay 2 seconds @03:46:52.20 AS.reconfigure ASconfig @03:46:52.23 AS.seek 1.000ml @03:47:50.81 delay 1 second @03:47:51.82 AFV.select :holdingCoil @03:47:51.91 delay 1 second @03:47:52.92 AS.reconfigure ASfast @03:47:52.96 AS.seek 0.000ml @03:48:20.70 delay 3 seconds @03:48:23.71 AFV.select :carrierSolution @03:48:23.80 delay 2 seconds @03:48:25.81 AS.reconfigure ASconfig @03:48:25.84 AS.seek 1.000ml @03:49:24.41 delay 1 second @03:49:25.42 AFV.select :holdingCoil @03:49:25.51 delay 1 second @03:49:26.52 AS.reconfigure ASfast @03:49:26.55 AS.seek 0.000ml @03:49:54.29 delay 3 seconds @03:49:57.30 AFV.select :carrierSolution @03:49:57.39 delay 2 seconds @03:49:59.40 AS.reconfigure ASconfig @03:49:59.43 AS.seek 1.000ml @03:50:58.02 delay 1 second @03:50:59.03 AFV.select :holdingCoil @03:50:59.12 delay 1 second @03:51:00.13 AS.reconfigure ASnorm @03:51:00.16 AS.seek 0.000ml @03:51:51.61 AFV.select :carrierSolution @03:51:51.70 delay 2 seconds @03:51:53.71 AS.reconfigure ASconfig @03:51:53.74 AS.seek 1.000ml @03:52:52.34 delay 1 second @03:52:53.35 AFV.select :holdingCoil @03:52:53.44 delay 1 second @03:52:54.45 ATV.dial :waste @03:52:55.35 AS.reconfigure ASnorm @03:52:55.38 AS.seek 0.500ml @03:53:21.31 ATV.dial :waste2 @03:53:22.41 AS.seek 0.000ml @03:53:48.34
Cleaning PCR AFV.select :carrierSolution @03:53:48.43 delay 2 seconds @03:53:50.44 AS.reconfigure ASconfig @03:53:50.47 AS.seek 0.500ml @03:54:19.90 delay 1 second @03:54:20.91 AFV.select :holdingCoil @03:54:21.00 ASV.dial :waste1 @03:54:21.01 ATV.dial :air3 @03:54:22.50 AS.reconfigure ASslow1 @03:54:22.53 AS.seek 0.510ml @03:54:24.87 delay 2 seconds @03:54:26.88 ATV.dial :bleach @03:54:27.26 AS.seek 0.810ml @03:55:28.69 delay 3 seconds @03:55:31.70 ATV.dial :air3 @03:55:32.12 delay 3 seconds @03:55:35.18 AS.seek 0.835ml @03:55:40.51 delay 3 seconds @03:55:43.52 ATV.dial :bleach @03:55:43.92 AS.seek 0.820ml @03:55:47.21 delay 3 seconds @03:55:50.22 ARV.dial :waste @03:55:50.73 delay 3 seconds @03:55:53.74 ATV.dial :ARV @03:55:54.47 AS.reconfigure ASconfig @03:55:54.51 AS.seek 0.470ml @03:56:15.19 AFV.select :carrierSolution @03:56:15.28 delay 2 seconds @03:56:17.29 AS.seek 1.000ml @03:56:48.49 delay 1 second @03:56:49.50 AFV.select :holdingCoil @03:56:49.59 delay 1 second @03:56:50.60 AS.reconfigure ASfast @03:56:50.63 AS.seek 0.000ml @03:57:18.35 AFV.select :carrierSolution @03:57:18.44 delay 2 seconds @03:57:20.45 AS.reconfigure ASconfig @03:57:20.48 AS.seek 1.000ml @03:58:19.08 delay 1 second @03:58:20.09 AFV.select :holdingCoil @03:58:20.18 delay 1 second @03:58:21.19 AS.reconfigure ASfast @03:58:21.22 AS.seek 0.000ml @03:58:48.95 AFV.select :carrierSolution @03:58:49.04 delay 2 seconds @03:58:51.05 AS.reconfigure ASconfig @03:58:51.08 AS.seek 1.000ml @03:59:49.65 delay 1 second @03:59:50.66 AFV.select :holdingCoil @03:59:50.75 delay 1 second @03:59:51.76 AS.reconfigure ASfast @03:59:51.79 AS.seek 0.000ml @04:00:19.53 ARV.dial between carrier and air1 @04:00:20.04 ATV.dial :air3 @04:00:20.98 AS.reconfigure ASslow1 @04:00:21.02 AS.seek 0.010ml @04:00:23.36 delay 2 seconds @04:00:25.37 ATV.dial :bleach @04:00:25.77 AS.seek 0.810ml @04:03:09.15 delay 3 seconds @04:03:12.16 ATV.dial :air3 @04:03:12.51 delay 3 seconds @04:03:15.57 AS.seek 0.835ml @04:03:20.91 delay 3 seconds @04:03:23.92 ATV.dial :bleach @04:03:24.40 AS.seek 0.820ml @04:03:27.82 delay 3 seconds @04:03:30.83 ATV.dial PCR @04:03:31.28 AS.reconfigure ASconfig @04:03:31.31 AS.seek empty @04:04:19.46 PCR.program :bleachProfile @04:04:19.52 PCR program should complete in < 2:45 @04:04:19.53 PCR.run 10:00 @04:07:26.78 PCR.off @04:07:26.81 AFV.select :carrierSolution @04:07:26.90 delay 2 seconds @04:07:28.91 AS.seek 1.000ml @04:08:27.56 delay 1 second @04:08:28.57 AFV.select :holdingCoil @04:08:28.66 delay 1 second @04:08:29.67 AS.reconfigure ASfast @04:08:29.70 AS.seek 0.000ml @04:08:57.43 delay 3 seconds @04:09:00.45 AFV.select :carrierSolution @04:09:00.54 delay 2 seconds @04:09:02.55 AS.reconfigure ASconfig @04:09:02.58 AS.seek 1.000ml @04:10:01.18 delay 1 second @04:10:02.19 AFV.select :holdingCoil @04:10:02.28 delay 1 second @04:10:03.29 AS.reconfigure ASfast @04:10:03.33 AS.seek 0.000ml @04:10:31.07 delay 3 seconds @04:10:34.08 AFV.select :carrierSolution @04:10:34.17 delay 2 seconds @04:10:36.18 AS.reconfigure ASconfig @04:10:36.22 AS.seek 1.000ml @04:11:34.83 delay 1 second @04:11:35.85 AFV.select :holdingCoil @04:11:35.94 delay 1 second @04:11:36.95 AS.reconfigure ASfast @04:11:36.99 AS.seek 0.000ml @04:12:04.72 delay 3 seconds @04:12:07.74 AFV.select :carrierSolution @04:12:07.83 delay 2 seconds @04:12:09.84 AS.reconfigure ASconfig @04:12:09.87 AS.seek 1.000ml @04:13:08.48 delay 1 second @04:13:09.49 AFV.select :holdingCoil @04:13:09.58 delay 1 second @04:13:10.59 AS.reconfigure ASfast @04:13:10.62 AS.seek 0.000ml @04:13:38.36 delay 3 seconds @04:13:41.38 AFV.select :carrierSolution @04:13:41.47 delay 2 seconds @04:13:43.48 AS.reconfigure ASconfig @04:13:43.51 AS.seek 1.000ml @04:14:42.09 delay 1 second @04:14:43.10 AFV.select :holdingCoil @04:14:43.19 delay 1 second @04:14:44.20 AS.reconfigure ASnorm @04:14:44.23 AS.seek 0.000ml @04:15:35.67 Completed {wqPCR} begun 13:27:43 @04:15:35.69 AS.stop @04:15:35.71
Asleep til +0:10 @04:15:35.73 Sleeping until 04:15 @04:15:35.76 GoodNews.email "Then, in tube 3: coreNegNTCphase\n/home/esp/esp2/mission/14mar14sccwrp.rb:59",Subject => "Asleep until 04:15" @04:15:37.57 CC.stop @04:15:37.60 CS.stop @04:15:37.63 PC.stop @04:15:37.66 PS.stop @04:15:37.69 Forearm.stop @04:15:37.72 Elbow.stop @04:15:37.75 SS.stop @04:15:37.82 Sleepy.power :raw,:OFF @04:15:37.83 Sleepy.power :dwarf,:OFF Shutdown @04:15:45.99
Powering up Dwarves @04:15:51.23 Sleepy.power :raw,:ON @04:15:51.69 SPE.configure SPEconfig @04:15:51.71 AS.configure ASconfig @04:15:51.74 CC.configure CCconfig @04:15:51.77 CS.configure CSconfig @04:15:51.80 PC.configure PCconfig @04:15:51.83 PS.configure PSconfig @04:15:51.86 Forearm.configure LooseForearm @04:15:51.89 Elbow.configure LooseElbow @04:15:51.92 SC.configure SCloose @04:15:51.95 SE.configure SEconfig @04:15:51.98 SS.configure SSconfig @04:15:52.01 CH.configure CHconfig @04:15:52.03 PH.configure PHconfig @04:15:52.06 Valve.reset @04:15:52.73 PCR.off @04:15:52.76 PCR.pidtemp= 50,0.1,0,300,25,25 @04:15:52.79 PCR.caltemp= 6.6661,0.0344,0.0 @04:15:52.90 Intake.select :close @04:15:52.91 Exhaust.select :close @04:15:52.92 CTV.dial :air @04:15:52.96 PSV.dial :air @04:15:52.99
PC.reconfigure PCconfig @04:15:53.05 PC.home.seek open @04:15:53.07 CSV.dial :waste1 @04:15:53.44 PS.reconfigure PSconfig @04:15:53.48 PS.home.seek 0.000ml @04:15:53.52 SSV.dial :spit,avoiding => exhaust @04:15:54.30 CS.reconfigure CSfast @04:15:54.33 SS.reconfigure SSconfig @04:15:54.42 SS.home.seek 0.00ml @04:15:54.44 CS.home.seek 0.000ml @04:15:54.92 CC.reconfigure CCconfig @04:15:54.96 CC.home.seek open @04:16:03.69 PS.coast @04:16:03.77
CC.seek open @04:16:03.79 PC.seek open @04:16:03.95
Forearm.reconfigure LooseForearm @04:16:03.98 Forearm.home.seek retract @04:16:04.72 Hand.grip @04:16:04.74
Elbow.jog -1500 @04:16:05.26 Elbow.reconfigure LooseElbow @04:16:05.29 Elbow.home.seek clear @04:16:10.01 SE.reconfigure SEconfig @04:16:10.04 SE.home.seek down @04:16:10.62 SC.reconfigure SCsloppy @04:16:10.66 SC.home.seek initial @04:16:14.43 Forearm.seek retract @04:16:14.48 Elbow.seek hammer @04:16:15.66 Forearm.reconfigure TightForearm @04:16:15.71 Forearm.seek 2 @04:16:16.83 Forearm.seek retract @04:16:17.84 Forearm.reconfigure LooseForearm @04:16:18.22PDT15-Mar-14 ===> STARTED coreNegNTCWCR (phase #12) <=== @04:16:18.23 Starting initialPurge @04:16:18.24 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @04:16:18.29 Hand.release @04:16:19.08 Elbow.reconfigure TightElbow @04:16:19.12 Elbow.seek FlushPuck::Garage @04:16:20.84 Elbow.reconfigure LooseElbow @04:16:20.93 Forearm.seek garage @04:16:21.94 Hand.grip @04:16:22.71 Forearm.seek retract @04:16:23.71 Elbow.reconfigure TightElbow @04:16:23.75 Elbow.seek CC @04:16:26.57 Elbow.reconfigure LooseElbow @04:16:26.66 Forearm.reconfigure TightForearm @04:16:26.69 Forearm.seek CC @04:16:27.72 CC.seek closed @04:16:31.50 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.97 @04:16:31.55 Hand.release @04:16:32.29 Forearm.reconfigure LooseForearm @04:16:32.32 Forearm.seek retract @04:16:33.25 Flushing the Collection Valve::Manifold @04:16:33.35 CS Pulling 5ml of flush CSV.dial CSR @04:16:34.62 CSR.select :flush @04:16:34.72 CS.reconfigure CSconfig @04:16:34.76 CS.seek 5.000ml @04:16:48.16 delay 5 seconds @04:16:53.18 CSR.select :air @04:16:53.32 CS.reconfigure CSfast @04:16:53.35 CS.seek 7.500ml @04:16:59.94 CSV.dial :waste @04:17:01.19 CS.reconfigure CSconfig @04:17:01.23 CS.seek 0.000ml @04:17:21.07 Purging external Intake @04:17:21.16 CSV.dial :flush @04:17:21.81 CS.reconfigure CSfast @04:17:21.84 CS.seek 10.000ml @04:17:45.35 delay 5 seconds @04:17:50.36 CSV.dial CBV @04:17:50.38
CBV.dial CSV @04:17:51.74 CTV.dial :intake @04:17:52.47 Intake.select :open @04:17:52.57 CS.seek 0.000ml @04:18:16.32 Intake.select :close @04:18:16.41 CTV.dial :air @04:18:17.16 Flushing upper manifold @04:18:17.25 CSV.dial :flush @04:18:17.97 CS.seek 5.000ml @04:18:30.07 delay 5 seconds @04:18:35.08 CSV.dial :air @04:18:35.55 CS.seek 7.000ml @04:18:40.88 CSV.dial CBV @04:18:40.90
CBV.dial CSV @04:18:41.97 CTV.dial :air @04:18:41.99 CS.seek 0.000ml @04:18:58.60 delay 7 seconds @04:19:05.62 CS.seek 3.000ml @04:19:13.25 CSV.dial :waste @04:19:13.76 CS.seek 0.000ml @04:19:21.64 CSV.dial :flush @04:19:22.07 CS.seek 3.000ml @04:19:29.69 delay 5 seconds @04:19:34.70 CSV.dial :air @04:19:35.18 CS.seek 5.000ml @04:19:40.50 CSV.dial :air @04:19:40.51 CS.seek 0.000ml @04:19:52.56 delay 7 seconds @04:19:59.57 CS.seek 3.000ml @04:20:07.15 CSV.dial :waste @04:20:07.95 CS.seek 0.000ml @04:20:15.59 Garaging FlushPuck in Collection Clamp @04:20:15.65 Elbow.reconfigure TightElbow @04:20:15.68 Elbow.seek CC @04:20:15.91 Elbow.reconfigure LooseElbow @04:20:16.00 Forearm.seek CC @04:20:16.97 CC.seek sealed @04:20:17.90 Hand.grip @04:20:18.61 CC.seek open @04:20:21.90 Forearm.seek retract @04:20:22.84 Elbow.reconfigure TightElbow @04:20:22.88 Elbow.seek garage @04:20:25.69 Elbow.reconfigure LooseElbow @04:20:26.30 Forearm.reconfigure TightForearm @04:20:26.33 Forearm.seek garage @04:20:27.49 Hand.release @04:20:28.28 Forearm.reconfigure LooseForearm @04:20:28.31 Forearm.seek retract @04:20:29.43 Starting mfb @04:20:29.44 pcrBAC wcr sampling at most 1000ml @04:20:29.45 BACfans.email "Can@04:17:02, 16.2C, 69% humidity, 14.3psia, 13.694V, 0.714A, 0.720A avg, 9.77W\nCTD data unavailable\nISUS data unavailable",Subject => "pcrBAC wcr sampling at most 1000ml" @04:20:31.20
Loading sh1 puck from tube 3 into Collection Clamp @04:20:31.31 Readying the MFB @04:20:31.32 Homing MFB Syringe @04:20:31.34 AFV.select :holdingCoil @04:20:31.47
SC.reconfigure SCtight @04:20:31.54 SC.seek 3 @04:20:31.58 ATV.dial :air2 @04:20:32.98 AS.reconfigure ASconfig @04:20:33.01 AS.home.seek home @04:20:33.78
SC.reconfigure SCloose @04:20:33.97 Elbow.reconfigure TightElbow @04:20:34.00 Elbow.seek 3 @04:20:35.92 Forearm.reconfigure TightForearm @04:20:35.95 Forearm.seek 3 + 800 counts @04:20:36.99 SE.reconfigure SEpick @04:20:37.05 SE.seek beamBreak @04:20:38.96 SE.configure SEconfig @04:20:39.09 Elbow.seek 3 @04:20:39.22 Forearm.seek 3 + 300 counts @04:20:39.48 Forearm.reconfigure LooseForearm @04:20:39.52 Hand.grip @04:20:40.25 SE.seek down @04:20:40.39
Forearm.seek retract @04:20:40.51 AFV.select :carrierSolution @04:20:40.62 delay 2 seconds @04:20:41.39
Elbow.seek CC @04:20:42.01 SE.coast @04:20:42.63 AS.seek 1.000ml @04:20:42.96
Elbow.reconfigure LooseElbow @04:20:43.04 Forearm.reconfigure TightForearm @04:20:43.07 Forearm.seek CC @04:20:44.19 CC.seek closed @04:20:47.95 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.99 @04:20:47.96 Hand.release @04:20:48.78 Forearm.reconfigure LooseForearm @04:20:48.81 Forearm.seek retract @04:20:49.76 SC.coast @04:20:49.79 skipping prime and sample collection @04:20:49.80 Evacuating Puck (bubblePt=55.0psi, delay=15.0s) Intake.select :close @04:20:49.86 CSV.dial :waste @04:20:49.89 CS.seek 0.000ml @04:20:50.02
Calibrating pressure and temperature sensors @04:20:50.04 CSV.dial :air @04:20:50.05
CTV.dial :air @04:20:50.07 SSV.dial :spit,avoiding => exhaust @04:20:50.09 SS.reconfigure SSfast @04:20:50.13 SS.seek 5.00ml @04:20:50.85 CS.seek 10.000ml @04:20:53.01 SSV.dial CBV @04:20:53.03
CBV.dial SSV @04:20:54.38 delay 3.8 seconds @04:20:58.26 SSV.dial :spit @04:20:59.57 SS.seek 0.00ml @04:21:02.23 SSV.dial CBV @04:21:03.54 SSV.dial CBV,avoiding => exhaust @04:21:03.55
CBV.dial SSV @04:21:03.56 CTV.dial :air @04:21:04.04 delay 15 seconds @04:21:19.04 SSV.dial :spit,avoiding => exhaust @04:21:20.35 CTV.dial CSV,avoiding => intake @04:21:20.37
SS.reconfigure SSexhale @04:21:20.44 SS.seek 0.00ml @04:21:20.49 SSV.dial CBV,avoiding => exhaust @04:21:21.84 CBV.dial between CSV and waste2b @04:21:22.77 CTV.dial CSV,avoiding => air @04:21:22.78
CSV.dial CTV @04:21:23.22 CS.reconfigure CSpressurize @04:21:23.25 CS.seek 0.000ml @04:21:34.34 delay 1 second @04:21:35.35 AFV.select :holdingCoil @04:21:35.44 delay 1 second @04:21:36.45 ATV.dial :waste @04:21:37.36 AS.reconfigure ASnorm @04:21:37.39 AS.home.seek empty @04:21:49.12
CSV.dial :air @04:21:49.64 CS.reconfigure CSfast @04:21:49.67 CS.seek 10.000ml @04:22:13.13 CSV.dial between air and CTV @04:22:14.11 CS.seek 3.239ml @04:22:30.16 CTV.dial CSV,avoiding => air @04:22:30.17
CSV.dial CTV @04:22:30.53 CS.reconfigure CSpressurize @04:22:30.56 CS.seek 0.000ml @04:22:30.68 ATV.dial :waste @04:22:30.69 ARV.dial between carrier and air1 @04:22:30.76 ASV.dial :waste1 @04:22:38.82
BubblePoint: Intake @69.4psia, Outlet @14.3psia @04:22:38.83 SS.reconfigure SSconfig @04:22:38.86 SS.seek 10.00ml @04:22:44.60 delay 15 seconds @04:22:59.70 Evacuated: Intake @68.7psia, Outlet @-0.2psia SSV.dial :spit,avoiding => exhaust @04:23:01.01 SS.reconfigure SSexhale @04:23:01.04 SS.seek 0.00ml @04:23:05.89 SS.reconfigure SSfast @04:23:05.92 SS.seek 5.00ml @04:23:08.73 SSV.dial CBV,avoiding => exhaust @04:23:08.75
CBV.dial SSV @04:23:10.08 CTV.dial :air,avoiding => intake @04:23:11.38 CSV.dial CTV @04:23:11.40
SS.reconfigure SSconfig @04:23:11.44 SS.seek 10.00ml @04:23:12.40 CSV.dial :air @04:23:12.92 CS.reconfigure CSfast @04:23:12.95 CS.seek 0.000ml @04:23:14.12 CS.coast @04:23:14.63
SSV.dial :spit,avoiding => exhaust @04:23:15.93 SS.reconfigure SSexhale @04:23:15.96 SS.seek 0.00ml @04:23:20.94 SS.reconfigure SSfast @04:23:20.97 SS.seek 5.00ml @04:23:23.81 SSV.dial CBV,avoiding => exhaust @04:23:23.83
CBV.dial SSV @04:23:26.13 SSV.dial :spit,avoiding => exhaust @04:23:27.43 SS.reconfigure SSconfig @04:23:27.46 SS.seek 0.00ml @04:23:30.60 SS.coast @04:23:30.63 Evacuating Puck (bubblePt=55.0psi, delay=15.0s) Intake.select :close @04:23:30.69 CSV.dial :waste @04:23:30.78 SSV.dial CBV,avoiding => exhaust @04:23:30.81
CBV.dial SSV @04:23:31.44 CS.seek 0.000ml @04:23:31.80 CSV.dial :air @04:23:32.11
CTV.dial :air @04:23:32.12 delay 15 seconds @04:23:32.59 CS.seek 10.000ml @04:23:47.12
SSV.dial :spit,avoiding => exhaust @04:23:48.43 CTV.dial CSV,avoiding => intake @04:23:48.45
SS.reconfigure SSexhale @04:23:48.52 SS.seek 0.00ml @04:23:48.57 SSV.dial CBV,avoiding => exhaust @04:23:56.29 CBV.dial between CSV and waste2b @04:23:57.21 CTV.dial CSV,avoiding => air @04:23:57.23
CSV.dial CTV @04:23:57.65 CS.reconfigure CSpressurize @04:23:57.68 CS.seek 0.000ml @04:24:23.55 CSV.dial :air @04:24:24.06 CS.reconfigure CSfast @04:24:24.09 CS.seek 10.000ml @04:24:47.53 CSV.dial between air and CTV @04:24:47.90 CS.seek 3.230ml @04:25:03.93 CTV.dial CSV,avoiding => air @04:25:03.94
CSV.dial CTV @04:25:04.23 CS.reconfigure CSpressurize @04:25:04.26 CS.seek 0.000ml @04:25:12.56 BubblePoint: Intake @69.8psia, Outlet @14.5psia @04:25:12.57 SS.reconfigure SSconfig @04:25:12.61 SS.seek 10.00ml @04:25:18.21 delay 15 seconds @04:25:33.31 Evacuated: Intake @69.0psia, Outlet @0.1psia SSV.dial :spit,avoiding => exhaust @04:25:35.12 SS.reconfigure SSexhale @04:25:35.16 SS.seek 0.00ml @04:25:40.05 SS.reconfigure SSfast @04:25:40.09 SS.seek 5.00ml @04:25:42.91 SSV.dial CBV,avoiding => exhaust @04:25:42.93
CBV.dial SSV @04:25:44.27 CTV.dial :air,avoiding => intake @04:25:45.57 CSV.dial CTV @04:25:45.59
SS.reconfigure SSconfig @04:25:45.63 SS.seek 10.00ml @04:25:46.59 CSV.dial :air @04:25:47.10 CS.reconfigure CSfast @04:25:47.13 CS.seek 0.000ml @04:25:48.21 CS.coast @04:25:48.82
SSV.dial :spit,avoiding => exhaust @04:25:50.14 SS.reconfigure SSexhale @04:25:50.17 SS.seek 0.00ml @04:25:55.80 SS.reconfigure SSfast @04:25:55.84 SS.seek 5.00ml @04:25:58.76 SSV.dial CBV,avoiding => exhaust @04:25:58.78
CBV.dial SSV @04:26:01.08 SSV.dial :spit,avoiding => exhaust @04:26:02.39 SS.reconfigure SSconfig @04:26:02.42 SS.seek 0.00ml @04:26:05.50 SS.coast @04:26:05.53 Lysing: Adding 1.5ml lysis CBV.dial between CSV and waste1,avoiding => DBV @04:26:07.00 CTV.dial CSV @04:26:08.26 CSV.dial CSR @04:26:09.04 CSR.select :lysis @04:26:09.14 CS.reconfigure CSconfig @04:26:09.18 CS.seek 1.000ml @04:26:12.49 delay 5 seconds @04:26:17.51 CSR.select :air @04:26:17.65 CS.seek 6.000ml @04:26:31.09 delay 2 seconds @04:26:33.10 CSV.dial :waste1 @04:26:34.36 CS.reconfigure CSfast @04:26:34.39 CS.seek empty @04:26:48.65 CSV.dial CSR @04:26:49.91 CSR.select :lysis @04:26:50.01 CS.reconfigure CSconfig @04:26:50.05 CS.seek 1.500ml @04:26:54.65 delay 5 seconds @04:26:59.67 CSR.select :air @04:26:59.81 CS.seek 4.499ml @04:27:08.18 delay 2 seconds @04:27:10.20 CSV.dial CTV @04:27:10.70 CS.seek 1.275ml @04:27:19.62 Heating sh1 puck from tube 3 in Collection Clamp to 85.0C for 5:00 @04:27:19.63 CH.seek 85.0C,8:00 @04:29:52.11 CH.hold 5:00,85.0C @04:34:52.21 Collection Puck Heater at 85.0C after lysing @04:34:52.22 CS.seek 0.000ml @04:34:56.32 CTV.dial between spare and air,avoiding => intake @04:34:57.31 CSV.dial :air @04:34:57.76 CS.seek 5.000ml @04:35:11.31 CSV.dial CTV @04:35:11.83 delay 2 seconds @04:35:13.84 CSV.dial :air @04:35:14.36 delay 2 seconds @04:35:16.37 CSV.dial CTV @04:35:16.87 delay 2 seconds @04:35:18.88 CSV.dial :air @04:35:19.37 delay 2 seconds @04:35:21.38 CS.seek 0.000ml @04:35:34.87 Recovering Lysate into Collection syringe @04:35:34.88 CSV.dial CBV @04:35:35.86 CBV.dial CSV @04:35:36.20 CS.seek 5.000ml @04:35:49.69 delay 5 seconds @04:35:54.70 CTV.dial :air @04:35:55.19 CS.seek 7.000ml @04:36:01.09 delay 5 seconds @04:36:06.10 CSV.dial :air2 @04:36:06.54 CS.seek 10.000ml @04:36:14.90 CSV.dial between air and CTV,avoiding => CBV @04:36:16.02 Lysing Complete @04:36:16.07 Storing sh1 puck in Collection Clamp to tube 2 @04:36:16.20 SC.reconfigure SCtight @04:36:16.23 SC.seek 2 @04:36:18.40 SC.reconfigure SCloose @04:36:18.56 Elbow.reconfigure TightElbow @04:36:18.59 Elbow.seek 2 @04:36:20.19 Forearm.reconfigure TightForearm @04:36:20.22 Forearm.seek 2 + 800 counts @04:36:21.32 SE.reconfigure SEpick @04:36:21.37 SE.seek beamBreak @04:36:23.32 SE.configure SEconfig @04:36:23.43 Elbow.reconfigure LimpElbow @04:36:23.47 Forearm.reconfigure LooseForearm @04:36:23.51 Forearm.seek 2 @04:36:23.77 Elbow.hold @04:36:23.82 Elbow.reconfigure TightElbow @04:36:23.90 SE.seek 10.03 Pucks below Top Plate @04:36:24.20 Forearm.coast @04:36:24.23 Forearm.seek 2 + 321 counts @04:36:24.47 Forearm.reconfigure TightForearm @04:36:24.50 Forearm.seek 2 + 800 counts @04:36:24.80 SE.seek 11.03 Pucks below Top Plate @04:36:25.48 Forearm.reconfigure LooseForearm @04:36:25.51 Forearm.seek retract @04:36:26.45 Elbow.seek CC @04:36:28.05 Elbow.reconfigure LooseElbow @04:36:28.14 Forearm.seek CC @04:36:29.11 CC.seek sealed @04:36:30.03 Hand.grip @04:36:30.70 CC.seek open @04:36:34.38 Forearm.seek retract @04:36:35.54 Elbow.reconfigure TightElbow @04:36:35.58 Elbow.seek 2 @04:36:37.13 Elbow.reconfigure LimpElbow @04:36:37.18 Forearm.seek 2 @04:36:38.19 Elbow.hold @04:36:38.24 Elbow.reconfigure TightElbow @04:36:38.28 Hand.release @04:36:39.03 Forearm.coast @04:36:39.06 Forearm.seek 2 + 306 counts @04:36:39.29 Forearm.reconfigure TightForearm @04:36:39.32 Forearm.seek 2 + 800 counts @04:36:39.60 SE.seek down @04:36:40.70
Forearm.reconfigure LooseForearm @04:36:40.74 Forearm.seek retract @04:36:41.26 SE.coast @04:36:41.65
SC.coast @04:36:41.68 Tranferring lysate to PRV @04:36:41.69 CSV.dial :air @04:36:41.99 CS.seek 10.000ml @04:36:42.06 CSV.dial between air and CTV @04:36:42.44 AIV.dial :bypass @04:36:42.51 Beginning samplePassC @04:36:42.52 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @04:36:42.66 Elbow.seek FlushPuck::Garage @04:36:44.45 Elbow.reconfigure LooseElbow @04:36:44.53 Forearm.seek garage @04:36:45.54 Hand.grip @04:36:46.42 Forearm.seek retract @04:36:47.44 Elbow.reconfigure TightElbow @04:36:47.48 Elbow.seek CC @04:36:50.30 Elbow.reconfigure LooseElbow @04:36:50.39 Forearm.reconfigure TightForearm @04:36:50.42 Forearm.seek CC @04:36:51.45 CC.seek closed @04:36:55.24 Collection Clamp onSpring/offSpring for 5/22 samples, on/off torque 2.05 @04:36:55.29 Hand.release @04:36:56.12 Forearm.reconfigure LooseForearm @04:36:56.15 Forearm.seek retract @04:36:57.08 AWV.select :air @04:36:57.09 AIV.dial :bypass @04:36:57.10 PSV.dial PSR @04:36:58.37 PSR.select :flush @04:36:58.47 PS.seek 1.500ml @04:37:14.53 delay 5 seconds @04:37:19.54 PSV.dial :waste1 @04:37:19.99 PS.seek 0.000ml @04:37:36.06 PSV.dial PSR @04:37:36.50 PSR.select :air @04:37:36.60 PS.seek 2.500ml @04:38:02.88 CBV.dial PRV @04:38:02.89 PRV.dial CBV @04:38:03.65
PSV.dial :mixloop @04:38:04.78 CTV.dial :air,avoiding => intake @04:38:04.79 PTV.dial :PRVmixing @04:38:04.87 PS.seek 0.000ml @04:38:31.12 PSV.dial :air @04:38:31.58 PS.seek 2.500ml @04:38:57.74 PSV.dial :mixloop @04:38:58.19 PS.seek 0.000ml @04:39:24.43 PSV.dial :air @04:39:25.00 PS.seek 2.500ml @04:39:51.27 PSV.dial :mixloop @04:39:51.70 PS.seek 0.000ml @04:40:17.90 Garaging FlushPuck in Collection Clamp @04:40:17.96 Elbow.reconfigure TightElbow @04:40:17.99 Elbow.seek CC @04:40:18.24 Elbow.reconfigure LooseElbow @04:40:18.33 Forearm.seek CC @04:40:19.29 CC.seek sealed @04:40:20.20 Hand.grip @04:40:20.94 CC.seek open @04:40:24.79 Forearm.seek retract @04:40:25.78 Elbow.reconfigure TightElbow @04:40:25.82 Elbow.seek garage @04:40:28.58 Elbow.reconfigure LooseElbow @04:40:28.66 Forearm.reconfigure TightForearm @04:40:28.69 Forearm.seek garage @04:40:29.89 Hand.release @04:40:30.61 Forearm.reconfigure LooseForearm @04:40:30.64 Forearm.seek retract @04:40:31.67 Connecting PS to CS to transfer sample @04:40:31.68 CBV.dial AIV @04:40:31.69 AIV.dial :bypass @04:40:31.70 PSV.dial :mixloop @04:40:31.71 PTV.dial :PRVmixing @04:40:31.72 PRV.dial AIV CTV.dial :air,avoiding => intake @04:40:31.73 Loading lyfil puck from tube 3 into Collection Clamp @04:40:31.91 SC.reconfigure SCtight @04:40:31.94 SC.seek 3 @04:40:34.11 SC.reconfigure SCloose @04:40:34.28 Elbow.reconfigure TightElbow @04:40:34.31 Elbow.seek 3 @04:40:36.15 Forearm.reconfigure TightForearm @04:40:36.18 Forearm.seek 3 + 800 counts @04:40:37.16 SE.reconfigure SEpick @04:40:37.21 SE.seek beamBreak @04:40:39.28 SE.configure SEconfig @04:40:39.40 Elbow.seek 3 @04:40:39.54 Forearm.seek 3 + 300 counts @04:40:39.76 Forearm.reconfigure LooseForearm @04:40:39.80 Hand.grip @04:40:40.57 SE.seek down @04:40:40.72
Forearm.seek retract @04:40:41.68 Elbow.seek CC @04:40:42.44 SE.coast @04:40:43.20
Elbow.reconfigure LooseElbow @04:40:43.28 Forearm.reconfigure TightForearm @04:40:43.31 Forearm.seek CC @04:40:44.42 CC.seek closed @04:40:48.23 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.96 @04:40:48.24 Hand.release @04:40:48.98 Forearm.reconfigure LooseForearm @04:40:49.01 Forearm.seek retract @04:40:49.93 SC.coast @04:40:49.96 CTV.dial CSV,avoiding => intake @04:40:51.25 CSV.dial CTV @04:40:51.54 CS.seek 5.000ml @04:41:04.95 delay 1:20 @04:42:24.97 CS.seek 2.500ml @04:42:32.08 delay 40 seconds @04:43:12.09 CS.seek 0.000ml @04:43:19.24 delay 1:00 @04:44:19.25 PSV.dial :air @04:44:19.69 PS.seek 2.500ml @04:44:45.99 PSV.dial :mixloop @04:44:46.43 delay 3 seconds @04:44:49.44 PSV.dial :air @04:44:49.88 delay 2 seconds @04:44:51.89 PSV.dial :mixloop @04:44:52.32 delay 3 seconds @04:44:55.33 PSV.dial :waste1 @04:44:56.73 PS.seek empty @04:45:23.18 CBV.dial :waste1 @04:45:23.64 delay 5 seconds @04:45:28.65 CBV.dial AIV @04:45:29.11 delay 10 seconds @04:45:39.12 CBV.dial :waste1 @04:45:39.59 delay 10 seconds @04:45:49.60 CBV.dial AIV @04:45:50.06 delay 10 seconds @04:46:00.07 CTV.dial CSV,avoiding => intake @04:46:00.08 CBV.dial between AIV and waste1 @04:46:00.40 CSV.dial :air @04:46:01.13 CS.seek 10.000ml @04:46:27.23 CSV.dial CTV @04:46:27.66 CS.seek 0.000ml @04:46:53.76 CBV.dial :waste1 @04:46:54.06 delay 10 seconds @04:47:04.07 CTV.dial :air,avoiding => intake @04:47:05.42 Storing lyfil puck in Collection Clamp to tube 2 @04:47:05.55 SC.reconfigure SCtight @04:47:05.58 SC.seek 2 @04:47:07.82 SC.reconfigure SCloose @04:47:08.53 Elbow.reconfigure TightElbow @04:47:08.56 Elbow.seek 2 @04:47:10.14 Forearm.reconfigure TightForearm @04:47:10.17 Forearm.seek 2 + 800 counts @04:47:11.26 SE.reconfigure SEpick @04:47:11.31 SE.seek beamBreak @04:47:13.19 SE.configure SEconfig @04:47:13.31 Elbow.reconfigure LimpElbow @04:47:13.34 Forearm.reconfigure LooseForearm @04:47:13.38 Forearm.seek 2 @04:47:13.61 Elbow.hold @04:47:13.66 Elbow.reconfigure TightElbow @04:47:13.74 SE.seek 11.03 Pucks below Top Plate @04:47:14.04 Forearm.coast @04:47:14.07 Forearm.seek 2 + 321 counts @04:47:14.35 Forearm.reconfigure TightForearm @04:47:14.38 Forearm.seek 2 + 800 counts @04:47:14.64 SE.seek 12.03 Pucks below Top Plate @04:47:15.24 Forearm.reconfigure LooseForearm @04:47:15.28 Forearm.seek retract @04:47:16.20 Elbow.seek CC @04:47:17.76 Elbow.reconfigure LooseElbow @04:47:17.84 Forearm.seek CC @04:47:18.75 CC.seek sealed @04:47:19.70 Hand.grip @04:47:20.44 CC.seek open @04:47:23.79 Forearm.seek retract @04:47:24.74 Elbow.reconfigure TightElbow @04:47:24.78 Elbow.seek 2 @04:47:26.34 Elbow.reconfigure LimpElbow @04:47:26.38 Forearm.seek 2 @04:47:27.39 Elbow.hold @04:47:27.44 Elbow.reconfigure TightElbow @04:47:27.48 Hand.release @04:47:28.26 Forearm.coast @04:47:28.30 Forearm.seek 2 + 320 counts @04:47:28.53 Forearm.reconfigure TightForearm @04:47:28.56 Forearm.seek 2 + 800 counts @04:47:28.87 SE.seek down @04:47:29.98
Forearm.reconfigure LooseForearm @04:47:30.02 Forearm.seek retract @04:47:30.39 SE.coast @04:47:30.93
SC.coast @04:47:30.96 PSV.dial :air @04:47:32.02 PS.seek 0.499ml @04:47:37.95 delay 3 seconds @04:47:40.96 PSV.dial :mixloop @04:47:41.39 PRV.dial :air @04:47:41.84 delay 3 seconds @04:47:44.85 PRV.dial CBV @04:47:45.30 CBV.dial AIV @04:47:45.76 PRV.dial between CBV and air @04:47:46.08 PRV.dial :air @04:47:46.41 PS.reconfigure PSslow @04:47:46.44 PS.seek 0.599ml @04:47:48.92 PRV.dial :waste2 @04:47:49.65 PS.reconfigure PSconfig @04:47:49.68 PS.seek empty @04:47:56.61 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @04:47:56.76 Elbow.seek FlushPuck::Garage @04:47:58.51 Elbow.reconfigure LooseElbow @04:47:58.60 Forearm.seek garage @04:47:59.61 Hand.grip @04:48:00.39 Forearm.seek retract @04:48:01.42 Elbow.reconfigure TightElbow @04:48:01.46 Elbow.seek CC @04:48:04.21 Elbow.reconfigure LooseElbow @04:48:04.30 Forearm.reconfigure TightForearm @04:48:04.33 Forearm.seek CC @04:48:05.43 CC.seek closed @04:48:09.16 Collection Clamp onSpring/offSpring for 5/22 samples, on/off torque 2.05 @04:48:09.21 Hand.release @04:48:09.97 Forearm.reconfigure LooseForearm @04:48:10.00 Forearm.seek retract @04:48:10.99 Clearing PML for modified lysate @04:48:11.00 CTV.dial :air,avoiding => intake PRV.dial :waste2 @04:48:11.01 PTV.dial :PRV,avoiding => puck @04:48:11.74 delay 2 seconds @04:48:13.74 PTV.dial :PRVmixing,avoiding => puck @04:48:14.47 delay 2 seconds @04:48:16.48 PSV.dial :PML @04:48:16.49 delay 2 seconds @04:48:18.49 PRV.dial :air @04:48:19.22 delay 2 seconds @04:48:21.28 PS.seek 1.499ml @04:48:37.39 delay 5 seconds @04:48:42.40 PS.seek 2.500ml @04:48:53.45 PSV.dial :air @04:48:53.91 PS.seek 0.000ml @04:49:20.17 mixing lysate with speDil @04:49:20.18 PSV.dial :PML @04:49:20.75 CBV.dial :PRV @04:49:20.76 PRV.dial :air @04:49:20.77 delay 2 seconds @04:49:22.78 PRV.dial :speDil @04:49:23.21 delay 2 seconds @04:49:25.27 PS.reconfigure PSslow1 @04:49:25.30 PS.seek 0.225ml @04:49:48.64 delay 5 seconds @04:49:53.65 PRV.dial CBV @04:49:54.39 delay 2 seconds @04:49:56.45 PS.seek 0.475ml @04:50:22.20 delay 5 seconds @04:50:27.21 PRV.dial :air @04:50:27.65 delay 2 seconds @04:50:29.66 PS.seek 0.925ml @04:51:15.95 delay 10 seconds @04:51:25.96 PRV.dial CBV @04:51:26.40 CTV.dial :air,avoiding => intake @04:51:26.41 PSV.dial :air @04:51:26.85 PS.reconfigure PSconfig @04:51:26.88 PS.seek 2.500ml @04:51:43.69 delay 3 seconds @04:51:46.70 PSV.dial PTV @04:51:47.41 PTV.dial :PRV,avoiding => puck @04:51:48.13 PS.seek 0.000ml @04:52:14.38 PSV.dial :air @04:52:15.11 PS.seek 2.500ml @04:52:41.32 delay 3 seconds @04:52:44.33 PSV.dial PTV @04:52:45.05 PTV.dial :PRV,avoiding => puck @04:52:45.07 PS.seek 0.000ml @04:53:11.28 Pushing modified lysate into AIV Injection Loop @04:53:11.29 CBV.dial AIV @04:53:11.30 AIV.dial :bypass @04:53:11.31 PRV.dial CBV @04:53:11.32 PSV.dial :air @04:53:12.04 PS.seek 2.500ml @04:53:38.31 delay 3 seconds @04:53:41.33 PSV.dial :PML @04:53:41.77 PTV.dial :PRVmixing,avoiding => puck @04:53:42.55 PS.reconfigure PSslow1 @04:53:42.58 PS.seek 1.850ml @04:54:49.00 delay 5 seconds @04:54:54.01 AIV.dial :sample @04:54:54.53 AWV.select :waste @04:54:54.67 PS.reconfigure PSslow2 @04:54:54.70 PS.seek 1.310ml @04:56:45.28 delay 15 seconds @04:57:00.29 AIV.dial :bypass @04:57:00.84 PS.reconfigure PSconfig @04:57:00.87 PS.seek 0.000ml @04:57:14.99 PSV.dial :air @04:57:15.44 PS.seek 2.500ml @04:57:41.71 PSV.dial :PML @04:57:42.16 PS.seek 0.000ml @04:58:08.37 AIV.dial :coilToMFB @04:58:08.38 AWV.select :air @04:58:08.52 Extracting Nucleic Acid from AIV @04:58:08.54
Beginning samplePassD @04:58:08.55 SPE.hold 2:46:40,55.0C @04:58:08.57 ASV.dial :SPEdelivery @04:58:08.70
AWV.select :air @04:58:08.71 AIV.dial :bypass PSV.dial PSR @04:58:09.12 delay 20 seconds @04:58:09.87
PSR.select :killMFB @04:58:09.97 PS.seek 1.500ml @04:58:26.02 delay 5 seconds @04:58:29.13 primeLysateINT @04:58:29.14 AWV.select :air @04:58:29.15 AIV.dial :coilToMFB ATV.dial :AIV @04:58:30.57 AS.reconfigure ASslow1 @04:58:30.60 AS.seek 0.239ml @04:58:31.03
PSV.dial :waste1 @04:58:31.61 PS.seek 0.000ml @04:58:47.68 PSV.dial PSR @04:58:48.12 PSR.select :air @04:58:48.22 PS.seek 2.500ml @04:59:14.50 PRV.dial CBV @04:59:14.52
CBV.dial PRV @04:59:14.53 PSV.dial :mixloop @04:59:16.03 CTV.dial :air,avoiding => intake @04:59:16.04 PTV.dial :PRVmixing @04:59:16.06 PS.seek 0.000ml @04:59:19.88 delay 10 seconds @04:59:29.89 ATV.dial :air2 @04:59:30.47 AS.seek 0.269ml @04:59:36.75 ATV.dial :waste2 @04:59:37.10 AS.reconfigure ASnorm @04:59:37.13 AS.seek empty @04:59:42.34
PSV.dial PSR @04:59:43.39 PSR.select :flush @04:59:43.49 PS.seek 1.500ml @04:59:51.28 Lysate primed! @04:59:51.29 AFV.select :holdingCoil @04:59:51.30 ATV.dial :air2 @04:59:51.69 AS.reconfigure ASslow1 @04:59:51.72 AS.seek 0.069ml @04:59:59.69
delay 5 seconds @05:00:04.70 PSV.dial :waste1 @05:00:05.15 PS.seek 0.000ml @05:00:06.30 delay 3 seconds @05:00:09.31 ATV.dial :AIV @05:00:09.89 AS.seek 0.469ml @05:00:21.25
PSV.dial PSR @05:00:21.70 PSR.select :air @05:00:21.80 PS.seek 2.500ml @05:00:48.12 PRV.dial CBV @05:00:48.14
CBV.dial PRV @05:00:48.15 PSV.dial :mixloop @05:00:49.29 CTV.dial :air,avoiding => intake @05:00:49.30 PTV.dial :PRVmixing @05:00:49.32 PS.seek 0.000ml @05:01:15.56 PSV.dial PSR @05:01:16.57 PSR.select :flush @05:01:16.67 PS.seek 1.500ml @05:01:31.72 delay 5 seconds @05:01:32.78
delay 5 seconds @05:01:36.77 Solid Phase Extraction Heater at 52.6C @05:01:36.78 ATV.dial ASV @05:01:37.11 ASV.dial :SPEdelivery @05:01:37.17 AS.seek 0.041ml @05:01:37.79
PSV.dial :waste1 @05:01:38.24 PS.seek 0.000ml @05:01:54.31 PSV.dial PSR @05:01:54.76 PSR.select :air @05:01:54.86 PS.seek 2.500ml @05:02:21.07 PRV.dial CBV @05:02:21.08
CBV.dial PRV @05:02:21.09 PSV.dial :mixloop @05:02:22.21 CTV.dial :air,avoiding => intake @05:02:22.22 PTV.dial :PRVmixing @05:02:22.24 PS.seek 0.000ml @05:02:48.52 PSV.dial PSR @05:02:49.52 PSR.select :flush @05:02:49.62 PS.seek 1.500ml @05:03:04.71 delay 10 seconds @05:03:05.74
delay 5 seconds @05:03:10.75 PSV.dial :waste1 @05:03:11.20 PS.seek 0.000ml @05:03:14.72 rinseLysateLineINT @05:03:14.73 AFV.select :carrierSolution @05:03:14.82 delay 2 seconds @05:03:16.83 AS.reconfigure ASconfig @05:03:16.86 AS.seek 1.000ml @05:03:27.27
PSV.dial PSR @05:03:27.72 PSR.select :air @05:03:27.82 PS.seek 2.500ml @05:03:53.99 PRV.dial CBV @05:03:54.01
CBV.dial PRV @05:03:54.02 PSV.dial :mixloop @05:03:55.02 CTV.dial :air,avoiding => intake @05:03:55.03 PTV.dial :PRVmixing @05:03:55.04 PS.reconfigure PSslow @05:03:55.08 PS.seek 1.200ml @05:04:13.01 delay 1 second @05:04:14.02 AFV.select :holdingCoil @05:04:14.11 delay 1 second @05:04:15.12 ATV.dial :AIV @05:04:15.46 AIV.dial :coilToMFB @05:04:15.47 AWV.select :waste @05:04:15.57 AS.reconfigure ASslow0 @05:04:15.61 AS.seek 0.000ml @05:04:22.02
PSV.dial :waste1 @05:04:23.33 PS.reconfigure PSconfig @05:04:23.36 PS.seek 0.000ml @05:04:36.36 PSV.dial :air @05:04:37.39 PS.seek 2.500ml @05:05:03.71 PSV.dial :mixloop @05:05:04.15 PS.reconfigure PSslow @05:05:04.18 PS.seek 0.000ml @05:05:55.70 PSV.dial :air @05:05:56.15 PS.reconfigure PSconfig @05:05:56.18 PS.seek 2.500ml @05:05:57.96 ATV.dial :air2 @05:05:58.62 AS.reconfigure ASslow1 @05:05:58.65 AS.seek 0.050ml @05:06:09.11 ATV.dial :AIV @05:06:09.68 AS.seek 0.010ml @05:06:18.07 ATV.dial :waste @05:06:19.36 AS.seek empty @05:06:21.94 ethanol wash @05:06:21.95 ATV.dial :air2 @05:06:22.36
PSV.dial :mixloop @05:06:22.90 AS.reconfigure ASslow2 @05:06:22.94
PS.reconfigure PSslow @05:06:22.96 AS.seek 0.009ml @05:06:23.02
PS.seek 0.000ml @05:06:33.36 delay 3 seconds @05:06:36.37 Pulling 0.4ml of Ethanol Wash @05:06:36.38 ATV.dial :ethanol @05:06:36.94 AS.reconfigure ASslow1 @05:06:37.45 AS.seek 0.409ml @05:07:14.36
Cleaning Collection Syringe @05:07:14.37 CSV.dial CSR @05:07:14.79 CSR.select :flush @05:07:14.89 CS.seek 4.000ml @05:07:25.93 delay 5 seconds @05:07:30.94 CSR.select :air @05:07:31.03 CS.seek 10.000ml @05:07:46.99 CSV.dial CTV @05:07:47.01
CTV.dial CSV @05:07:48.35 CBV.dial :waste1 @05:07:48.83 CS.seek 0.000ml @05:07:59.30 delay 3 seconds @05:08:02.31 ATV.dial :air1 @05:08:02.75 delay 3 seconds @05:08:05.81 AS.seek 0.434ml @05:08:11.08 delay 3 seconds @05:08:14.09 ATV.dial :ethanol @05:08:14.47 AS.seek 0.419ml @05:08:14.93
CSV.dial CSR @05:08:15.43 CSR.select :flush @05:08:15.53 CS.seek 4.000ml @05:08:17.80 delay 3 seconds @05:08:20.81 ATV.dial ASV @05:08:21.94 AS.seek 0.092ml @05:08:26.49
delay 5 seconds @05:08:31.50 CSR.select :air @05:08:31.59 CS.seek 10.000ml @05:08:47.55 CSV.dial CTV @05:08:47.57
CTV.dial CSV @05:08:48.07 CBV.dial :waste1 @05:08:48.09 CS.seek 0.000ml @05:09:14.29 Garaging FlushPuck in Collection Clamp @05:09:14.34 Elbow.reconfigure TightElbow @05:09:14.37 Elbow.seek CC @05:09:14.48 Elbow.reconfigure LooseElbow @05:09:14.56 Forearm.seek CC @05:09:15.51 CC.seek sealed @05:09:16.52 Hand.grip @05:09:17.26 CC.seek open @05:09:20.55 Forearm.seek retract @05:09:21.49 Elbow.reconfigure TightElbow @05:09:21.53 Elbow.seek garage @05:09:24.26 Elbow.reconfigure LooseElbow @05:09:24.34 Forearm.reconfigure TightForearm @05:09:24.37 Forearm.seek garage @05:09:25.52 Hand.release @05:09:26.30 Forearm.reconfigure LooseForearm @05:09:26.33 Forearm.seek retract @05:09:27.39 Whole Cell Archive sampling 1000ml @05:09:27.41 Loading wcr puck from tube 3 into Collection Clamp @05:09:27.59 SC.reconfigure SCtight @05:09:27.62 SC.seek 3 @05:09:28.87 delay 3 seconds @05:09:29.81 SC.reconfigure SCloose @05:09:29.99 Elbow.reconfigure TightElbow @05:09:30.02 Elbow.seek 3 @05:09:31.85 Forearm.reconfigure TightForearm @05:09:31.88 Forearm.seek 3 + 800 counts @05:09:31.92 ATV.dial :waste2 @05:09:32.56 AS.reconfigure ASconfig @05:09:32.59 AS.seek empty @05:09:32.91 SE.reconfigure SEpick @05:09:32.96 SE.seek beamBreak @05:09:35.14 SE.configure SEconfig @05:09:35.27 Elbow.seek 3 @05:09:35.42 Forearm.seek 3 + 300 counts @05:09:35.71 Forearm.reconfigure LooseForearm @05:09:35.75 Hand.grip @05:09:36.52 SE.seek down @05:09:36.66 Forearm.seek retract @05:09:37.59 Elbow.seek CC @05:09:38.31 ATV.dial :air2 @05:09:38.51 SE.coast @05:09:38.66 AS.reconfigure ASslow1 @05:09:38.69 AS.seek 1.000ml @05:09:39.10 Elbow.reconfigure LooseElbow @05:09:39.18 Forearm.reconfigure TightForearm @05:09:39.21 Forearm.seek CC @05:09:40.77 CC.seek closed @05:09:44.58 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.99 @05:09:44.59 Hand.release @05:09:45.43 Forearm.reconfigure LooseForearm @05:09:45.46 Forearm.seek retract @05:09:46.41 SC.coast @05:09:46.44 collectSample 1000ml w/bubble point of 55psi @05:09:46.46 Priming 7ml Sleepy.power :sampler,:ON @05:09:46.47 Intake.select :close @05:09:46.48 Exhaust.select :close @05:09:46.49 SS.hold @05:09:46.64 Calibrating pressure sensors @05:09:46.65 CTV.dial :air @05:09:47.97 SSV.dial :spit,avoiding => exhaust @05:09:47.98 SS.reconfigure SSfast @05:09:48.01 SS.seek 5.00ml @05:09:50.81 SSV.dial CBV @05:09:50.82 CBV.dial SSV @05:09:52.25 delay 3.8 seconds @05:09:56.14 SSV.dial :spit @05:09:57.49 SS.seek 0.00ml @05:10:00.17 SSV.dial CBV @05:10:01.53 CTV.dial :intake @05:10:02.27 Waiting for external sampler to finish priming... @05:10:02.28 delayUntil "05:19:46.46" @05:13:03.12 delay 5 seconds @05:13:08.13 ATV.dial ASV @05:13:08.85 ASV.dial :SPEdelivery @05:13:08.87 AS.seek 0.020ml @05:16:29.06 delay 5 seconds @05:16:34.07 ATV.dial :air2 @05:16:34.84 AS.seek 0.520ml @05:18:17.10 delay 5 seconds @05:18:22.11 ATV.dial ASV @05:18:22.82 ASV.dial :wasteR @05:18:23.14 AS.seek 0.300ml @05:19:08.26 delay 20 seconds @05:19:28.27 AS.seek 0.070ml @05:19:46.47 SSV.dial CBV @05:19:46.49 CBV.dial SSV @05:19:46.50 Allowing 55s for 7.0ml of remaining 7.0ml @05:19:46.51 Intake.select :open @05:19:48.64 Intake @51.0psia, vacuum from 20.0 to 24.0psi @05:19:48.66 SS.reconfigure forSampling @05:19:48.69 SS.seek 7.00ml @05:19:55.13 Stroke completed -- waiting for pressure to equalize ... @05:19:55.77 Intake.select :close @05:19:55.90 SSV.dial :exhaust @05:19:56.68 Exhaust.select :open @05:19:56.78 SS.reconfigure SSexhale @05:19:56.82 SS.seek 0.00ml @05:20:00.37 Exhaust.select :close @05:20:00.48 Sampling 1000.0ml @05:20:00.49 Intake.select :close @05:20:00.50 Exhaust.select :close @05:20:00.51 SS.reconfigure SSconfig @05:20:00.54 SS.hold @05:20:00.69 CTV.dial :intake @05:20:00.70 SSV.dial CBV @05:20:00.72 CBV.dial SSV @05:20:01.52 Allowing 185s for 25.0ml of remaining 1000.0ml @05:20:01.53 Intake.select :open @05:20:03.66 Intake @49.4psia, vacuum from 20.0 to 24.0psi @05:20:03.68 SS.reconfigure forSampling @05:20:03.71 SS.seek 25.00ml @05:20:15.43 delay 20 seconds @05:20:18.15 Stroke completed -- waiting for pressure to equalize ... @05:20:18.79 Intake.select :close @05:20:18.93 SSV.dial :exhaust @05:20:19.71 Exhaust.select :open @05:20:19.81 SS.reconfigure SSexhale @05:20:19.85 SS.seek 0.00ml @05:20:28.71 Exhaust.select :close @05:20:28.81 SSV.dial CBV @05:20:28.83 CBV.dial SSV @05:20:29.62 Allowing 185s for 25.0ml of remaining 975.0ml @05:20:29.63 Intake.select :open @05:20:31.77 Intake @50.0psia, vacuum from 20.0 to 24.0psi @05:20:31.79 SS.reconfigure forSampling @05:20:31.82 SS.seek 25.00ml @05:20:35.44 ATV.dial :waste2 @05:20:35.98 AS.reconfigure ASconfig @05:20:36.01 AS.seek empty @05:20:40.14 Vacuum too high -- waiting up to 40s @05:20:40.42 Intake @41.5psia, vacuum from 20.0 to 24.0psi @05:20:40.43 SS.reconfigure forSampling @05:20:40.46 SS.seek 25.00ml @05:20:40.49 Elution @05:20:40.50 ATV.dial :air1 @05:20:41.49 AS.reconfigure ASslow1 @05:20:41.52 AS.seek 0.049ml @05:20:47.76 Stroke completed -- waiting for pressure to equalize ... @05:20:48.40 Intake.select :close @05:20:48.53 SSV.dial :exhaust @05:20:49.33 Exhaust.select :open @05:20:49.96 SS.reconfigure SSexhale @05:20:50.00 SS.seek 0.00ml @05:20:52.00 delay 3 seconds @05:20:55.01 ATV.dial :ElutionWater @05:20:55.58 AS.seek 0.109ml @05:20:58.96 Exhaust.select :close @05:20:59.06 SSV.dial CBV @05:20:59.08 CBV.dial SSV @05:20:59.88 Allowing 185s for 25.0ml of remaining 950.0ml @05:20:59.89 Intake.select :open @05:21:02.03 Intake @49.6psia, vacuum from 20.0 to 24.0psi @05:21:02.05 SS.reconfigure forSampling @05:21:02.08 SS.seek 25.00ml @05:21:06.25 Vacuum too high -- waiting up to 40s @05:21:06.52 Intake @42.9psia, vacuum from 20.0 to 24.0psi @05:21:06.54 SS.reconfigure forSampling @05:21:06.57 SS.seek 25.00ml @05:21:08.08 delay 3 seconds @05:21:11.09 ATV.dial ASV,via => air2 @05:21:12.80 ASV.dial :SPEelution @05:21:13.47 AFV.select :carrierSolution @05:21:13.62 AS.reconfigure ASnorm @05:21:13.65 AS.seek 0.759ml @05:21:19.29 Stroke completed -- waiting for pressure to equalize ... @05:21:19.93 Intake.select :close @05:21:20.07 SSV.dial :exhaust @05:21:20.86 Exhaust.select :open @05:21:20.96 SS.reconfigure SSexhale @05:21:21.00 SS.seek 0.00ml @05:21:29.74 Exhaust.select :close @05:21:29.84 SSV.dial CBV @05:21:29.86 CBV.dial SSV @05:21:30.66 Allowing 185s for 25.0ml of remaining 925.0ml @05:21:30.67 Intake.select :open @05:21:32.81 Intake @49.6psia, vacuum from 20.0 to 24.0psi @05:21:32.83 SS.reconfigure forSampling @05:21:32.86 SS.seek 25.00ml @05:21:36.36 Vacuum too high -- waiting up to 40s @05:21:36.63 Intake @44.3psia, vacuum from 20.0 to 24.0psi @05:21:36.64 SS.reconfigure forSampling @05:21:36.68 SS.seek 25.00ml @05:21:43.83 Vacuum too high -- waiting up to 40s @05:21:44.31 Intake @46.9psia, vacuum from 20.0 to 24.0psi @05:21:44.32 SS.reconfigure forSampling @05:21:44.36 SS.seek 25.00ml @05:21:47.16 delay 3 seconds @05:21:48.37 Vacuum too high -- waiting up to 40s @05:21:48.64 Intake @45.7psia, vacuum from 20.0 to 24.0psi @05:21:48.66 SS.reconfigure forSampling @05:21:48.69 SS.seek 25.00ml @05:21:50.17 AFV.select :holdingCoil @05:21:50.31 AS.reconfigure ASslow1 @05:21:50.34 AS.seek 0.489ml @05:21:57.63 Stroke completed -- waiting for pressure to equalize ... @05:21:58.27 Intake.select :close @05:21:58.41 SSV.dial :exhaust @05:21:59.19 Exhaust.select :open @05:21:59.29 SS.reconfigure SSexhale @05:21:59.33 SS.seek 0.00ml @05:22:08.16 Exhaust.select :close @05:22:08.26 SSV.dial CBV @05:22:08.28 CBV.dial SSV @05:22:09.08 Allowing 185s for 25.0ml of remaining 900.0ml @05:22:09.09 Intake.select :open @05:22:11.24 Intake @49.4psia, vacuum from 20.0 to 24.0psi @05:22:11.25 SS.reconfigure forSampling @05:22:11.28 SS.seek 25.00ml @05:22:13.49 Vacuum too high -- waiting up to 40s @05:22:13.76 Intake @46.3psia, vacuum from 20.0 to 24.0psi @05:22:13.78 SS.reconfigure forSampling @05:22:13.81 SS.seek 25.00ml @05:22:26.23 Vacuum too high -- waiting up to 40s @05:22:26.50 Intake @47.2psia, vacuum from 20.0 to 24.0psi @05:22:26.51 SS.reconfigure forSampling @05:22:26.55 SS.seek 25.00ml @05:22:45.64 delay 5 seconds @05:22:48.17 Stroke completed -- waiting for pressure to equalize ... @05:22:49.40 Intake.select :close @05:22:49.53 SSV.dial :exhaust @05:22:50.33 Exhaust.select :open @05:22:50.43 SS.reconfigure SSexhale @05:22:50.47 SS.seek 0.00ml @05:22:50.70 AS.reconfigure ASslow2 @05:22:50.73 AS.seek 0.454ml @05:22:59.30 Exhaust.select :close @05:22:59.40 SSV.dial CBV @05:22:59.42 CBV.dial SSV @05:23:00.22 Allowing 185s for 25.0ml of remaining 875.0ml @05:23:00.23 Intake.select :open @05:23:02.37 Intake @49.0psia, vacuum from 20.0 to 24.0psi @05:23:02.39 SS.reconfigure forSampling @05:23:02.42 SS.seek 25.00ml @05:23:05.24 Vacuum too high -- waiting up to 40s @05:23:05.50 Intake @47.8psia, vacuum from 20.0 to 24.0psi @05:23:05.51 SS.reconfigure forSampling @05:23:05.55 SS.seek 25.00ml @05:23:26.56 delay 1:00 @05:24:01.04 Stroke completed -- waiting for pressure to equalize ... @05:24:02.78 Intake.select :close @05:24:02.92 SSV.dial :exhaust @05:24:03.73 Exhaust.select :open @05:24:03.83 SS.reconfigure SSexhale @05:24:03.87 SS.seek 0.00ml @05:24:13.00 Exhaust.select :close @05:24:13.10 SSV.dial CBV @05:24:13.12 CBV.dial SSV @05:24:13.92 Allowing 185s for 25.0ml of remaining 850.0ml @05:24:13.93 Intake.select :open @05:24:16.08 Intake @49.2psia, vacuum from 20.0 to 24.0psi @05:24:16.09 SS.reconfigure forSampling @05:24:16.13 SS.seek 25.00ml @05:24:22.20 Vacuum too high -- waiting up to 40s @05:24:22.47 Intake @48.4psia, vacuum from 20.0 to 24.0psi @05:24:22.49 SS.reconfigure forSampling @05:24:22.52 SS.seek 25.00ml @05:24:26.57 pushing eluate to holding coil @05:24:26.63 AS.seek 0.294ml @05:26:02.12 Stroke completed -- waiting for pressure to equalize ... @05:26:03.92 Intake.select :close @05:26:04.06 SSV.dial :exhaust @05:26:04.87 Exhaust.select :open @05:26:04.97 SS.reconfigure SSexhale @05:26:05.01 SS.seek 0.00ml @05:26:13.86 Exhaust.select :close @05:26:13.96 SSV.dial CBV @05:26:13.98 CBV.dial SSV @05:26:14.79 Allowing 185s for 25.0ml of remaining 825.0ml @05:26:14.80 Intake.select :open @05:26:16.94 Intake @48.7psia, vacuum from 20.0 to 24.0psi @05:26:16.96 SS.reconfigure forSampling @05:26:16.99 SS.seek 25.00ml @05:27:10.24 delay 30 seconds @05:27:40.25 ATV.dial :waste,via => air3 @05:27:41.78 AS.reconfigure ASconfig @05:27:41.81 AS.seek empty @05:27:59.33 Parking Bleach in LysateSource line and SPEcolumn @05:27:59.34 ATV.dial :air3 @05:28:00.01 AS.reconfigure ASslow1 @05:28:00.04 AS.seek 0.029ml @05:28:06.44 ATV.dial :ElutionWater @05:28:06.84 AS.seek 0.059ml @05:28:07.19 Stroke completed -- waiting for pressure to equalize ... @05:28:10.17 Intake.select :close @05:28:10.30 SSV.dial :exhaust @05:28:11.11 Exhaust.select :open @05:28:11.21 SS.reconfigure SSexhale @05:28:11.25 SS.seek 0.00ml @05:28:13.22 ATV.dial :air3 @05:28:13.62 AS.seek 0.069ml @05:28:15.91 delay 2 seconds @05:28:17.92 ATV.dial :bleach @05:28:18.40 AS.seek 0.709ml @05:28:20.44 Exhaust.select :close @05:28:20.54 SSV.dial CBV @05:28:20.56 CBV.dial SSV @05:28:21.37 Allowing 185s for 25.0ml of remaining 800.0ml @05:28:21.38 Intake.select :open @05:28:23.51 Intake @48.9psia, vacuum from 20.0 to 24.0psi @05:28:23.53 SS.reconfigure forSampling @05:28:23.56 SS.seek 25.00ml @05:28:46.18 Vacuum too high -- waiting up to 40s @05:28:46.66 Intake @48.0psia, vacuum from 20.0 to 24.0psi @05:28:46.67 SS.reconfigure forSampling @05:28:46.71 SS.seek 25.00ml @05:28:57.62 Vacuum too high -- waiting up to 40s @05:28:58.10 Intake @47.9psia, vacuum from 20.0 to 24.0psi @05:28:58.11 SS.reconfigure forSampling @05:28:58.15 SS.seek 25.00ml @05:29:03.12 Vacuum too high -- waiting up to 40s @05:29:03.61 Intake @48.4psia, vacuum from 20.0 to 24.0psi @05:29:03.62 SS.reconfigure forSampling @05:29:03.65 SS.seek 25.00ml @05:29:08.52 Vacuum too high -- waiting up to 40s @05:29:09.00 Intake @48.1psia, vacuum from 20.0 to 24.0psi @05:29:09.01 SS.reconfigure forSampling @05:29:09.05 SS.seek 25.00ml @05:29:12.87 Vacuum too high -- waiting up to 40s @05:29:13.36 Intake @48.2psia, vacuum from 20.0 to 24.0psi @05:29:13.37 SS.reconfigure forSampling @05:29:13.41 SS.seek 25.00ml @05:29:16.41 Vacuum too high -- waiting up to 40s @05:29:16.89 Intake @48.2psia, vacuum from 20.0 to 24.0psi @05:29:16.90 SS.reconfigure forSampling @05:29:17.49 SS.seek 25.00ml @05:29:22.24 Vacuum too high -- waiting up to 40s @05:29:22.73 Intake @48.3psia, vacuum from 20.0 to 24.0psi @05:29:22.75 SS.reconfigure forSampling @05:29:22.78 SS.seek 25.00ml @05:29:25.53 Vacuum too high -- waiting up to 40s @05:29:26.02 Intake @48.4psia, vacuum from 20.0 to 24.0psi @05:29:26.04 SS.reconfigure forSampling @05:29:26.07 SS.seek 25.00ml @05:29:28.75 Vacuum too high -- waiting up to 40s @05:29:29.24 Intake @48.3psia, vacuum from 20.0 to 24.0psi @05:29:29.26 SS.reconfigure forSampling @05:29:29.29 SS.seek 25.00ml @05:29:32.37 Vacuum too high -- waiting up to 40s @05:29:32.87 Intake @48.5psia, vacuum from 20.0 to 24.0psi @05:29:32.88 SS.reconfigure forSampling @05:29:32.96 SS.seek 25.00ml @05:29:35.56 Vacuum too high -- waiting up to 40s @05:29:36.05 Intake @48.7psia, vacuum from 20.0 to 24.0psi @05:29:36.07 SS.reconfigure forSampling @05:29:36.10 SS.seek 25.00ml @05:29:38.31 Vacuum too high -- waiting up to 40s @05:29:38.80 Intake @48.0psia, vacuum from 20.0 to 24.0psi @05:29:38.82 SS.reconfigure forSampling @05:29:38.85 SS.seek 25.00ml @05:29:41.63 Vacuum too high -- waiting up to 40s @05:29:42.12 Intake @47.7psia, vacuum from 20.0 to 24.0psi @05:29:42.13 SS.reconfigure forSampling @05:29:42.16 SS.seek 25.00ml @05:29:44.53 Vacuum too high -- waiting up to 40s @05:29:45.02 Intake @47.7psia, vacuum from 20.0 to 24.0psi @05:29:45.04 SS.reconfigure forSampling @05:29:45.07 SS.seek 25.00ml @05:29:47.66 Vacuum too high -- waiting up to 40s @05:29:48.15 Intake @48.7psia, vacuum from 20.0 to 24.0psi @05:29:48.17 SS.reconfigure forSampling @05:29:48.20 SS.seek 25.00ml @05:29:50.47 Vacuum too high -- waiting up to 40s @05:29:50.97 SS.seek 25.00ml @05:29:52.97 Vacuum too high -- waiting up to 40s @05:29:53.46 SS.seek 25.00ml @05:29:55.59 Vacuum too high -- waiting up to 40s @05:29:56.07 Intake @48.0psia, vacuum from 20.0 to 24.0psi @05:29:56.09 SS.reconfigure forSampling @05:29:56.12 SS.seek 25.00ml @05:29:58.34 Vacuum too high -- waiting up to 40s @05:29:58.83 Intake @48.3psia, vacuum from 20.0 to 24.0psi @05:29:58.85 SS.reconfigure forSampling @05:29:58.88 SS.seek 25.00ml @05:30:01.09 Vacuum too high -- waiting up to 40s @05:30:01.57 Intake @48.3psia, vacuum from 20.0 to 24.0psi @05:30:01.59 SS.reconfigure forSampling @05:30:01.62 SS.seek 25.00ml @05:30:03.53 Vacuum too high -- waiting up to 40s @05:30:04.02 Intake @48.5psia, vacuum from 20.0 to 24.0psi @05:30:04.03 SS.reconfigure forSampling @05:30:04.07 SS.seek 25.00ml @05:30:06.22 Vacuum too high -- waiting up to 40s @05:30:06.70 Intake @48.0psia, vacuum from 20.0 to 24.0psi @05:30:06.72 SS.reconfigure forSampling @05:30:06.75 SS.seek 25.00ml @05:30:08.94 Vacuum too high -- waiting up to 40s @05:30:09.42 Intake @48.1psia, vacuum from 20.0 to 24.0psi @05:30:09.43 SS.reconfigure forSampling @05:30:09.47 SS.seek 25.00ml @05:30:11.47 Vacuum too high -- waiting up to 40s @05:30:11.95 Intake @48.1psia, vacuum from 20.0 to 24.0psi @05:30:11.96 SS.reconfigure forSampling @05:30:12.00 SS.seek 25.00ml @05:30:15.46 Vacuum too high -- waiting up to 40s @05:30:16.18 Intake @48.4psia, vacuum from 20.0 to 24.0psi @05:30:16.20 SS.reconfigure forSampling @05:30:16.23 SS.seek 25.00ml @05:30:18.50 Vacuum too high -- waiting up to 40s @05:30:18.99 Intake @48.8psia, vacuum from 20.0 to 24.0psi @05:30:19.01 SS.reconfigure forSampling @05:30:19.04 SS.seek 25.00ml @05:30:20.87 Vacuum too high -- waiting up to 40s @05:30:21.36 Intake @48.2psia, vacuum from 20.0 to 24.0psi @05:30:21.37 SS.reconfigure forSampling @05:30:21.41 SS.seek 25.00ml @05:30:23.53 Vacuum too high -- waiting up to 40s @05:30:24.02 Intake @48.3psia, vacuum from 20.0 to 24.0psi @05:30:24.04 SS.reconfigure forSampling @05:30:24.07 SS.seek 25.00ml @05:30:25.94 Vacuum too high -- waiting up to 40s @05:30:26.43 Intake @48.3psia, vacuum from 20.0 to 24.0psi @05:30:26.45 SS.reconfigure forSampling @05:30:26.48 SS.seek 25.00ml @05:30:28.40 Vacuum too high -- waiting up to 40s @05:30:28.89 Intake @48.0psia, vacuum from 20.0 to 24.0psi @05:30:28.91 SS.reconfigure forSampling @05:30:28.94 SS.seek 25.00ml @05:30:29.17 delay 3 seconds @05:30:30.87 Vacuum too high -- waiting up to 40s @05:30:31.58 Intake @48.5psia, vacuum from 20.0 to 24.0psi @05:30:31.60 SS.reconfigure forSampling @05:30:31.63 SS.seek 25.00ml @05:30:32.18 ATV.dial :air3 @05:30:32.54 delay 3 seconds @05:30:33.81 Vacuum too high -- waiting up to 40s @05:30:34.52 Intake @48.6psia, vacuum from 20.0 to 24.0psi @05:30:34.54 SS.reconfigure forSampling @05:30:34.57 SS.seek 25.00ml @05:30:35.59 AS.seek 0.734ml @05:30:36.52 Stroke completed -- waiting for pressure to equalize ... @05:30:40.09 Intake.select :close @05:30:40.22 SSV.dial :exhaust @05:30:40.95 delay 3 seconds @05:30:41.02 Exhaust.select :open @05:30:41.18 SS.reconfigure SSexhale @05:30:41.22 SS.seek 0.00ml @05:30:43.96 ATV.dial :bleach @05:30:44.46 AS.seek 0.719ml @05:30:47.77 delay 3 seconds @05:30:50.29 Exhaust.select :close @05:30:50.39 SSV.dial CBV @05:30:50.41 CBV.dial SSV @05:30:50.78 ATV.dial :AIV @05:30:51.22 Allowing 185s for 25.0ml of remaining 775.0ml @05:30:51.23 Intake.select :open @05:30:51.92 AS.reconfigure ASconfig @05:30:51.95 AS.seek 0.015ml @05:30:53.36 Intake @48.2psia, vacuum from 20.0 to 24.0psi @05:30:53.38 SS.reconfigure forSampling @05:30:53.41 SS.seek 25.00ml @05:30:56.64 Vacuum too high -- waiting up to 40s @05:30:57.12 Intake @48.4psia, vacuum from 20.0 to 24.0psi @05:30:57.13 SS.reconfigure forSampling @05:30:57.17 SS.seek 25.00ml @05:30:59.12 Vacuum too high -- waiting up to 40s @05:30:59.83 Intake @49.4psia, vacuum from 20.0 to 24.0psi @05:30:59.84 SS.reconfigure forSampling @05:30:59.88 SS.seek 25.00ml @05:31:01.81 Vacuum too high -- waiting up to 40s @05:31:02.30 Intake @48.6psia, vacuum from 20.0 to 24.0psi @05:31:02.32 SS.reconfigure forSampling @05:31:02.35 SS.seek 25.00ml @05:31:04.34 Vacuum too high -- waiting up to 40s @05:31:04.82 Intake @48.2psia, vacuum from 20.0 to 24.0psi @05:31:04.83 SS.reconfigure forSampling @05:31:04.87 SS.seek 25.00ml @05:31:08.30 Vacuum too high -- waiting up to 40s @05:31:09.01 Intake @48.5psia, vacuum from 20.0 to 24.0psi @05:31:09.03 SS.reconfigure forSampling @05:31:09.06 SS.seek 25.00ml @05:31:11.41 Vacuum too high -- waiting up to 40s @05:31:11.89 Intake @49.0psia, vacuum from 20.0 to 24.0psi @05:31:11.91 SS.reconfigure forSampling @05:31:11.94 SS.seek 25.00ml @05:31:13.93 Vacuum too high -- waiting up to 40s @05:31:14.42 Intake @49.0psia, vacuum from 20.0 to 24.0psi @05:31:14.44 SS.reconfigure forSampling @05:31:14.47 SS.seek 25.00ml @05:31:16.28 Vacuum too high -- waiting up to 40s @05:31:16.77 Intake @48.5psia, vacuum from 20.0 to 24.0psi @05:31:16.79 SS.reconfigure forSampling @05:31:16.82 SS.seek 25.00ml @05:31:18.62 Vacuum too high -- waiting up to 40s @05:31:19.11 Intake @48.4psia, vacuum from 20.0 to 24.0psi @05:31:19.13 SS.reconfigure forSampling @05:31:19.16 SS.seek 25.00ml @05:31:20.84 Vacuum too high -- waiting up to 40s @05:31:21.34 Intake @48.3psia, vacuum from 20.0 to 24.0psi @05:31:21.35 SS.reconfigure forSampling @05:31:21.38 SS.seek 25.00ml @05:31:23.12 Vacuum too high -- waiting up to 40s @05:31:23.83 Intake @48.9psia, vacuum from 20.0 to 24.0psi @05:31:23.84 SS.reconfigure forSampling @05:31:23.88 SS.seek 25.00ml @05:31:25.81 Vacuum too high -- waiting up to 40s @05:31:26.30 Intake @48.8psia, vacuum from 20.0 to 24.0psi @05:31:26.32 SS.reconfigure forSampling @05:31:26.35 SS.seek 25.00ml @05:31:28.00 Vacuum too high -- waiting up to 40s @05:31:28.49 Intake @49.2psia, vacuum from 20.0 to 24.0psi @05:31:28.50 SS.reconfigure forSampling @05:31:28.54 SS.seek 25.00ml @05:31:30.06 Vacuum too high -- waiting up to 40s @05:31:30.54 Intake @48.4psia, vacuum from 20.0 to 24.0psi @05:31:30.56 SS.reconfigure forSampling @05:31:30.59 SS.seek 25.00ml @05:31:32.21 Vacuum too high -- waiting up to 40s @05:31:32.70 Intake @48.6psia, vacuum from 20.0 to 24.0psi @05:31:32.71 SS.reconfigure forSampling @05:31:32.75 SS.seek 25.00ml @05:31:33.28 ATV.dial :waste @05:31:34.24 Vacuum too high -- waiting up to 40s @05:31:34.64 AS.seek empty @05:31:34.78 Intake @48.3psia, vacuum from 20.0 to 24.0psi @05:31:34.79 SS.reconfigure forSampling @05:31:34.83 SS.seek 25.00ml @05:31:35.83 ATV.dial :air3 @05:31:36.42 AS.reconfigure ASslow1 @05:31:36.45 AS.seek 0.009ml @05:31:36.50 Vacuum too high -- waiting up to 40s @05:31:37.00 Intake @48.9psia, vacuum from 20.0 to 24.0psi @05:31:37.02 SS.reconfigure forSampling @05:31:37.05 SS.seek 25.00ml @05:31:38.59 Vacuum too high -- waiting up to 40s @05:31:38.87 delay 2 seconds @05:31:39.08 SS.seek 25.00ml @05:31:40.46 Vacuum too high -- waiting up to 40s @05:31:40.93 ATV.dial :bleach @05:31:40.98 SS.seek 25.00ml @05:31:41.33 AS.seek 0.579ml @05:31:42.49 Vacuum too high -- waiting up to 40s @05:31:42.99 Intake @48.8psia, vacuum from 20.0 to 24.0psi @05:31:43.01 SS.reconfigure forSampling @05:31:43.04 SS.seek 25.00ml @05:31:44.53 Vacuum too high -- waiting up to 40s @05:31:45.40 Intake @48.7psia, vacuum from 20.0 to 24.0psi @05:31:45.42 SS.reconfigure forSampling @05:31:45.45 SS.seek 25.00ml @05:31:47.40 Vacuum too high -- waiting up to 40s @05:31:47.89 Intake @48.2psia, vacuum from 20.0 to 24.0psi @05:31:47.91 SS.reconfigure forSampling @05:31:47.94 SS.seek 25.00ml @05:31:49.59 Vacuum too high -- waiting up to 40s @05:31:50.08 Intake @48.7psia, vacuum from 20.0 to 24.0psi @05:31:50.10 SS.reconfigure forSampling @05:31:50.13 SS.seek 25.00ml @05:31:51.49 Vacuum too high -- waiting up to 40s @05:31:52.20 Intake @49.2psia, vacuum from 20.0 to 24.0psi @05:31:52.22 SS.reconfigure forSampling @05:31:52.25 SS.seek 25.00ml @05:31:53.68 Vacuum too high -- waiting up to 40s @05:31:54.17 Intake @48.5psia, vacuum from 20.0 to 24.0psi @05:31:54.18 SS.reconfigure forSampling @05:31:54.21 SS.seek 25.00ml @05:31:55.74 Vacuum too high -- waiting up to 40s @05:31:56.45 Intake @48.9psia, vacuum from 20.0 to 24.0psi @05:31:56.46 SS.reconfigure forSampling @05:31:56.50 SS.seek 25.00ml @05:31:58.02 Vacuum too high -- waiting up to 40s @05:31:58.51 Intake @48.5psia, vacuum from 20.0 to 24.0psi @05:31:58.52 SS.reconfigure forSampling @05:31:58.56 SS.seek 25.00ml @05:31:59.99 Vacuum too high -- waiting up to 40s @05:32:00.48 Intake @48.1psia, vacuum from 20.0 to 24.0psi @05:32:00.50 SS.reconfigure forSampling @05:32:00.53 SS.seek 25.00ml @05:32:01.99 Vacuum too high -- waiting up to 40s @05:32:02.70 Intake @48.6psia, vacuum from 20.0 to 24.0psi @05:32:02.72 SS.reconfigure forSampling @05:32:02.75 SS.seek 25.00ml @05:32:04.46 Vacuum too high -- waiting up to 40s @05:32:05.17 Intake @48.6psia, vacuum from 20.0 to 24.0psi @05:32:05.18 SS.reconfigure forSampling @05:32:05.22 SS.seek 25.00ml @05:32:07.00 Vacuum too high -- waiting up to 40s @05:32:07.50 Intake @48.3psia, vacuum from 20.0 to 24.0psi @05:32:07.51 SS.reconfigure forSampling @05:32:07.54 SS.seek 25.00ml @05:32:09.09 Vacuum too high -- waiting up to 40s @05:32:09.79 Intake @49.1psia, vacuum from 20.0 to 24.0psi @05:32:09.80 SS.reconfigure forSampling @05:32:09.84 SS.seek 25.00ml @05:32:11.40 Vacuum too high -- waiting up to 40s @05:32:11.89 Intake @48.6psia, vacuum from 20.0 to 24.0psi @05:32:11.90 SS.reconfigure forSampling @05:32:11.94 SS.seek 25.00ml @05:32:13.40 Vacuum too high -- waiting up to 40s @05:32:14.10 Intake @48.8psia, vacuum from 20.0 to 24.0psi @05:32:14.12 SS.reconfigure forSampling @05:32:14.15 SS.seek 25.00ml @05:32:15.71 Vacuum too high -- waiting up to 40s @05:32:16.20 Intake @48.7psia, vacuum from 20.0 to 24.0psi @05:32:16.21 SS.reconfigure forSampling @05:32:16.25 SS.seek 25.00ml @05:32:17.59 Vacuum too high -- waiting up to 40s @05:32:18.08 Intake @48.6psia, vacuum from 20.0 to 24.0psi @05:32:18.09 SS.reconfigure forSampling @05:32:18.13 SS.seek 25.00ml @05:32:19.40 Vacuum too high -- waiting up to 40s @05:32:19.89 Intake @48.4psia, vacuum from 20.0 to 24.0psi @05:32:19.90 SS.reconfigure forSampling @05:32:19.94 SS.seek 25.00ml @05:32:21.92 Vacuum too high -- waiting up to 40s @05:32:22.19 Intake @49.1psia, vacuum from 20.0 to 24.0psi @05:32:22.21 SS.reconfigure forSampling @05:32:22.24 SS.seek 25.00ml @05:32:23.96 Vacuum too high -- waiting up to 40s @05:32:24.45 Intake @48.9psia, vacuum from 20.0 to 24.0psi @05:32:24.47 SS.reconfigure forSampling @05:32:24.50 SS.seek 25.00ml @05:32:25.74 Vacuum too high -- waiting up to 40s @05:32:26.24 Intake @48.6psia, vacuum from 20.0 to 24.0psi @05:32:26.26 SS.reconfigure forSampling @05:32:26.29 SS.seek 25.00ml @05:32:27.62 Vacuum too high -- waiting up to 40s @05:32:28.33 Intake @48.9psia, vacuum from 20.0 to 24.0psi @05:32:28.35 SS.reconfigure forSampling @05:32:28.38 SS.seek 25.00ml @05:32:30.02 Vacuum too high -- waiting up to 40s @05:32:30.51 Intake @48.7psia, vacuum from 20.0 to 24.0psi @05:32:30.53 SS.reconfigure forSampling @05:32:30.56 SS.seek 25.00ml @05:32:31.80 Vacuum too high -- waiting up to 40s @05:32:32.51 Intake @48.9psia, vacuum from 20.0 to 24.0psi @05:32:32.53 SS.reconfigure forSampling @05:32:32.56 SS.seek 25.00ml @05:32:34.02 Vacuum too high -- waiting up to 40s @05:32:34.51 Intake @48.6psia, vacuum from 20.0 to 24.0psi @05:32:34.53 SS.reconfigure forSampling @05:32:34.56 SS.seek 25.00ml @05:32:35.93 Vacuum too high -- waiting up to 40s @05:32:36.64 Intake @49.0psia, vacuum from 20.0 to 24.0psi @05:32:36.66 SS.reconfigure forSampling @05:32:36.69 SS.seek 25.00ml @05:32:38.05 Vacuum too high -- waiting up to 40s @05:32:38.54 Intake @48.9psia, vacuum from 20.0 to 24.0psi @05:32:38.55 SS.reconfigure forSampling @05:32:38.59 SS.seek 25.00ml @05:32:39.77 Vacuum too high -- waiting up to 40s @05:32:40.25 Intake @48.6psia, vacuum from 20.0 to 24.0psi @05:32:40.26 SS.reconfigure forSampling @05:32:40.30 SS.seek 25.00ml @05:32:41.62 Vacuum too high -- waiting up to 40s @05:32:42.32 Intake @48.6psia, vacuum from 20.0 to 24.0psi @05:32:42.34 SS.reconfigure forSampling @05:32:42.37 SS.seek 25.00ml @05:32:43.77 Vacuum too high -- waiting up to 40s @05:32:44.48 SS.seek 25.00ml @05:32:45.83 Vacuum too high -- waiting up to 40s @05:32:46.31 Intake @48.4psia, vacuum from 20.0 to 24.0psi @05:32:46.32 SS.reconfigure forSampling @05:32:46.36 SS.seek 25.00ml @05:32:47.62 Vacuum too high -- waiting up to 40s @05:32:48.31 Intake @49.0psia, vacuum from 20.0 to 24.0psi @05:32:48.32 SS.reconfigure forSampling @05:32:48.36 SS.seek 25.00ml @05:32:49.65 Vacuum too high -- waiting up to 40s @05:32:50.35 Intake @49.3psia, vacuum from 20.0 to 24.0psi @05:32:50.36 SS.reconfigure forSampling @05:32:50.40 SS.seek 25.00ml @05:32:51.80 Vacuum too high -- waiting up to 40s @05:32:52.51 Intake @49.5psia, vacuum from 20.0 to 24.0psi @05:32:52.52 SS.reconfigure forSampling @05:32:52.56 SS.seek 25.00ml @05:32:53.93 Vacuum too high -- waiting up to 40s @05:32:54.40 Intake @48.4psia, vacuum from 20.0 to 24.0psi @05:32:55.02 SS.reconfigure forSampling @05:32:55.05 SS.seek 25.00ml @05:32:56.90 Vacuum too high -- waiting up to 40s @05:32:57.39 Intake @48.4psia, vacuum from 20.0 to 24.0psi @05:32:57.41 SS.reconfigure forSampling @05:32:57.44 SS.seek 25.00ml @05:32:58.74 Vacuum too high -- waiting up to 40s @05:32:59.45 Intake @49.4psia, vacuum from 20.0 to 24.0psi @05:32:59.47 SS.reconfigure forSampling @05:32:59.50 SS.seek 25.00ml @05:33:00.74 Vacuum too high -- waiting up to 40s @05:33:01.24 Intake @48.9psia, vacuum from 20.0 to 24.0psi @05:33:01.25 SS.reconfigure forSampling @05:33:01.29 SS.seek 25.00ml @05:33:02.46 Vacuum too high -- waiting up to 40s @05:33:03.17 Intake @49.2psia, vacuum from 20.0 to 24.0psi @05:33:03.18 SS.reconfigure forSampling @05:33:03.22 SS.seek 25.00ml @05:33:04.65 Vacuum too high -- waiting up to 40s @05:33:05.36 Intake @49.3psia, vacuum from 20.0 to 24.0psi @05:33:05.38 SS.reconfigure forSampling @05:33:05.41 SS.seek 25.00ml @05:33:06.74 Vacuum too high -- waiting up to 40s @05:33:07.22 Intake @48.7psia, vacuum from 20.0 to 24.0psi @05:33:07.24 SS.reconfigure forSampling @05:33:07.27 SS.seek 25.00ml @05:33:08.43 Vacuum too high -- waiting up to 40s @05:33:09.14 Intake @49.0psia, vacuum from 20.0 to 24.0psi @05:33:09.16 SS.reconfigure forSampling @05:33:09.19 SS.seek 25.00ml @05:33:10.71 Vacuum too high -- waiting up to 40s @05:33:11.21 Intake @48.5psia, vacuum from 20.0 to 24.0psi @05:33:11.22 SS.reconfigure forSampling @05:33:11.25 SS.seek 25.00ml @05:33:12.46 Vacuum too high -- waiting up to 40s @05:33:13.17 SS.seek 25.00ml @05:33:14.40 Vacuum too high -- waiting up to 40s @05:33:15.11 Intake @49.6psia, vacuum from 20.0 to 24.0psi @05:33:15.13 SS.reconfigure forSampling @05:33:15.16 SS.seek 25.00ml @05:33:16.43 Vacuum too high -- waiting up to 40s @05:33:16.92 Intake @49.1psia, vacuum from 20.0 to 24.0psi @05:33:16.94 SS.reconfigure forSampling @05:33:16.97 SS.seek 25.00ml @05:33:18.21 Vacuum too high -- waiting up to 40s @05:33:18.70 Intake @49.0psia, vacuum from 20.0 to 24.0psi @05:33:18.72 SS.reconfigure forSampling @05:33:18.75 SS.seek 25.00ml @05:33:19.81 Vacuum too high -- waiting up to 40s @05:33:20.51 Intake @48.6psia, vacuum from 20.0 to 24.0psi @05:33:20.52 SS.reconfigure forSampling @05:33:20.56 SS.seek 25.00ml @05:33:21.94 Vacuum too high -- waiting up to 40s @05:33:22.43 Intake @48.2psia, vacuum from 20.0 to 24.0psi @05:33:22.45 SS.reconfigure forSampling @05:33:22.48 SS.seek 25.00ml @05:33:23.75 Vacuum too high -- waiting up to 40s @05:33:24.45 Intake @48.4psia, vacuum from 20.0 to 24.0psi @05:33:24.46 SS.reconfigure forSampling @05:33:24.50 SS.seek 25.00ml @05:33:25.91 Vacuum too high -- waiting up to 40s @05:33:26.61 Intake @49.6psia, vacuum from 20.0 to 24.0psi @05:33:26.62 SS.reconfigure forSampling @05:33:26.66 SS.seek 25.00ml @05:33:28.97 Vacuum too high -- waiting up to 40s @05:33:29.46 Intake @49.0psia, vacuum from 20.0 to 24.0psi @05:33:29.48 SS.reconfigure forSampling @05:33:29.51 SS.seek 25.00ml @05:33:30.72 Vacuum too high -- waiting up to 40s @05:33:31.44 SS.seek 25.00ml @05:33:32.62 Vacuum too high -- waiting up to 40s @05:33:33.34 Intake @49.1psia, vacuum from 20.0 to 24.0psi @05:33:33.35 SS.reconfigure forSampling @05:33:33.38 SS.seek 25.00ml @05:33:34.72 Vacuum too high -- waiting up to 40s @05:33:35.21 Intake @48.7psia, vacuum from 20.0 to 24.0psi @05:33:35.23 SS.reconfigure forSampling @05:33:35.26 SS.seek 25.00ml @05:33:36.47 Vacuum too high -- waiting up to 40s @05:33:37.18 Intake @49.2psia, vacuum from 20.0 to 24.0psi @05:33:37.20 SS.reconfigure forSampling @05:33:37.23 SS.seek 25.00ml @05:33:37.88 delay 3 seconds @05:33:38.56 Vacuum too high -- waiting up to 40s @05:33:39.27 Intake @49.0psia, vacuum from 20.0 to 24.0psi @05:33:39.28 SS.reconfigure forSampling @05:33:39.32 SS.seek 25.00ml @05:33:40.72 Vacuum too high -- waiting up to 40s @05:33:40.93 ATV.dial :air3 @05:33:41.25 Intake @48.6psia, vacuum from 20.0 to 24.0psi @05:33:41.26 SS.reconfigure forSampling @05:33:41.29 SS.seek 25.00ml @05:33:41.39 delay 3 seconds @05:33:42.47 Vacuum too high -- waiting up to 40s @05:33:43.19 SS.seek 25.00ml @05:33:44.45 Vacuum too high -- waiting up to 40s @05:33:44.49 AS.seek 0.604ml @05:33:45.16 Intake @48.7psia, vacuum from 20.0 to 24.0psi @05:33:45.18 SS.reconfigure forSampling @05:33:45.21 SS.seek 25.00ml @05:33:45.71 Stroke completed -- waiting for pressure to equalize ... @05:33:49.28 Intake.select :close @05:33:49.42 SSV.dial :exhaust @05:33:49.90 delay 3 seconds @05:33:50.23 Exhaust.select :open @05:33:50.33 SS.reconfigure SSexhale @05:33:50.37 SS.seek 0.00ml @05:33:52.91 ATV.dial :bleach @05:33:53.30 AS.seek 0.589ml @05:33:56.70 delay 3 seconds @05:33:59.52 Exhaust.select :close @05:33:59.62 SSV.dial CBV @05:33:59.64 CBV.dial SSV @05:33:59.71 ATV.dial :ASV @05:34:00.45 Allowing 185s for 25.0ml of remaining 750.0ml @05:34:00.46 Intake.select :open @05:34:00.63 ASV.dial :wasteR @05:34:01.31 AS.seek 0.000ml @05:34:02.60 Intake @49.2psia, vacuum from 20.0 to 24.0psi @05:34:02.62 SS.reconfigure forSampling @05:34:02.65 SS.seek 25.00ml @05:34:05.03 Vacuum too high -- waiting up to 40s @05:34:05.52 Intake @49.0psia, vacuum from 20.0 to 24.0psi @05:34:05.54 SS.reconfigure forSampling @05:34:05.57 SS.seek 25.00ml @05:34:06.84 Vacuum too high -- waiting up to 40s @05:34:07.54 Intake @48.6psia, vacuum from 20.0 to 24.0psi @05:34:07.56 SS.reconfigure forSampling @05:34:07.59 SS.seek 25.00ml @05:34:09.15 Vacuum too high -- waiting up to 40s @05:34:10.45 Intake @48.8psia, vacuum from 20.0 to 24.0psi @05:34:10.47 SS.reconfigure forSampling @05:34:10.50 SS.seek 25.00ml @05:34:12.40 Vacuum too high -- waiting up to 40s @05:34:13.11 Intake @49.6psia, vacuum from 20.0 to 24.0psi @05:34:13.13 SS.reconfigure forSampling @05:34:13.16 SS.seek 25.00ml @05:34:14.40 Vacuum too high -- waiting up to 40s @05:34:15.11 Intake @49.4psia, vacuum from 20.0 to 24.0psi @05:34:15.13 SS.reconfigure forSampling @05:34:15.16 SS.seek 25.00ml @05:34:16.50 Vacuum too high -- waiting up to 40s @05:34:16.99 Intake @49.3psia, vacuum from 20.0 to 24.0psi @05:34:17.01 SS.reconfigure forSampling @05:34:17.04 SS.seek 25.00ml @05:34:18.09 Vacuum too high -- waiting up to 40s @05:34:18.58 Intake @49.0psia, vacuum from 20.0 to 24.0psi @05:34:18.60 SS.reconfigure forSampling @05:34:18.63 SS.seek 25.00ml @05:34:19.78 Vacuum too high -- waiting up to 40s @05:34:20.49 Intake @49.1psia, vacuum from 20.0 to 24.0psi @05:34:20.51 SS.reconfigure forSampling @05:34:20.54 SS.seek 25.00ml @05:34:21.87 Vacuum too high -- waiting up to 40s @05:34:22.58 Intake @49.3psia, vacuum from 20.0 to 24.0psi @05:34:22.60 SS.reconfigure forSampling @05:34:22.63 SS.seek 25.00ml @05:34:23.84 Vacuum too high -- waiting up to 40s @05:34:24.55 Intake @49.2psia, vacuum from 20.0 to 24.0psi @05:34:24.56 SS.reconfigure forSampling @05:34:24.60 SS.seek 25.00ml @05:34:26.03 Vacuum too high -- waiting up to 40s @05:34:26.74 Intake @49.4psia, vacuum from 20.0 to 24.0psi @05:34:26.75 SS.reconfigure forSampling @05:34:26.79 SS.seek 25.00ml @05:34:28.00 Vacuum too high -- waiting up to 40s @05:34:28.49 Intake @48.9psia, vacuum from 20.0 to 24.0psi @05:34:28.51 SS.reconfigure forSampling @05:34:28.54 SS.seek 25.00ml @05:34:29.62 Vacuum too high -- waiting up to 40s @05:34:30.33 Intake @49.6psia, vacuum from 20.0 to 24.0psi @05:34:30.34 SS.reconfigure forSampling @05:34:30.38 SS.seek 25.00ml @05:34:31.68 Vacuum too high -- waiting up to 40s @05:34:32.17 Intake @49.3psia, vacuum from 20.0 to 24.0psi @05:34:32.18 SS.reconfigure forSampling @05:34:32.22 SS.seek 25.00ml @05:34:33.31 Vacuum too high -- waiting up to 40s @05:34:33.80 Intake @48.5psia, vacuum from 20.0 to 24.0psi @05:34:33.82 SS.reconfigure forSampling @05:34:33.85 SS.seek 25.00ml @05:34:35.00 Vacuum too high -- waiting up to 40s @05:34:35.71 Intake @49.0psia, vacuum from 20.0 to 24.0psi @05:34:35.73 SS.reconfigure forSampling @05:34:35.76 SS.seek 25.00ml @05:34:37.12 Vacuum too high -- waiting up to 40s @05:34:37.81 Intake @49.3psia, vacuum from 20.0 to 24.0psi @05:34:37.82 SS.reconfigure forSampling @05:34:37.86 SS.seek 25.00ml @05:34:39.12 Vacuum too high -- waiting up to 40s @05:34:39.82 Intake @49.2psia, vacuum from 20.0 to 24.0psi @05:34:39.83 SS.reconfigure forSampling @05:34:39.87 SS.seek 25.00ml @05:34:41.12 Vacuum too high -- waiting up to 40s @05:34:41.99 Intake @49.9psia, vacuum from 20.0 to 24.0psi @05:34:42.01 SS.reconfigure forSampling @05:34:42.04 SS.seek 25.00ml @05:34:43.37 Vacuum too high -- waiting up to 40s @05:34:43.86 Intake @49.0psia, vacuum from 20.0 to 24.0psi @05:34:43.88 SS.reconfigure forSampling @05:34:43.91 SS.seek 25.00ml @05:34:45.09 Vacuum too high -- waiting up to 40s @05:34:45.80 Intake @49.2psia, vacuum from 20.0 to 24.0psi @05:34:45.82 SS.reconfigure forSampling @05:34:45.85 SS.seek 25.00ml @05:34:46.99 Vacuum too high -- waiting up to 40s @05:34:47.70 Intake @48.4psia, vacuum from 20.0 to 24.0psi @05:34:47.72 SS.reconfigure forSampling @05:34:47.75 SS.seek 25.00ml @05:34:49.06 Vacuum too high -- waiting up to 40s @05:34:49.77 Intake @48.6psia, vacuum from 20.0 to 24.0psi @05:34:49.79 SS.reconfigure forSampling @05:34:49.82 SS.seek 25.00ml @05:34:51.06 Vacuum too high -- waiting up to 40s @05:34:51.77 Intake @49.2psia, vacuum from 20.0 to 24.0psi @05:34:51.79 SS.reconfigure forSampling @05:34:51.82 SS.seek 25.00ml @05:34:52.99 Vacuum too high -- waiting up to 40s @05:34:53.70 Intake @49.8psia, vacuum from 20.0 to 24.0psi @05:34:53.71 SS.reconfigure forSampling @05:34:53.74 SS.seek 25.00ml @05:34:54.78 Vacuum too high -- waiting up to 40s @05:34:55.27 Intake @49.0psia, vacuum from 20.0 to 24.0psi @05:34:55.29 SS.reconfigure forSampling @05:34:55.32 SS.seek 25.00ml @05:34:56.37 Vacuum too high -- waiting up to 40s @05:34:57.08 Intake @48.9psia, vacuum from 20.0 to 24.0psi @05:34:57.09 SS.reconfigure forSampling @05:34:57.13 SS.seek 25.00ml @05:34:58.34 Vacuum too high -- waiting up to 40s @05:34:59.03 Intake @48.6psia, vacuum from 20.0 to 24.0psi @05:34:59.05 SS.reconfigure forSampling @05:34:59.08 SS.seek 25.00ml @05:35:00.34 Vacuum too high -- waiting up to 40s @05:35:01.05 SS.seek 25.00ml @05:35:02.11 Vacuum too high -- waiting up to 40s @05:35:02.82 Intake @48.7psia, vacuum from 20.0 to 24.0psi @05:35:02.84 SS.reconfigure forSampling @05:35:02.87 SS.seek 25.00ml @05:35:04.03 Vacuum too high -- waiting up to 40s @05:35:04.72 Intake @49.1psia, vacuum from 20.0 to 24.0psi @05:35:04.73 SS.reconfigure forSampling @05:35:04.77 SS.seek 25.00ml @05:35:05.90 Vacuum too high -- waiting up to 40s @05:35:06.60 Intake @49.4psia, vacuum from 20.0 to 24.0psi @05:35:06.62 SS.reconfigure forSampling @05:35:06.65 SS.seek 25.00ml @05:35:07.84 Vacuum too high -- waiting up to 40s @05:35:08.32 Intake @48.7psia, vacuum from 20.0 to 24.0psi @05:35:08.33 SS.reconfigure forSampling @05:35:08.37 SS.seek 25.00ml @05:35:09.37 Vacuum too high -- waiting up to 40s @05:35:09.86 Intake @48.5psia, vacuum from 20.0 to 24.0psi @05:35:09.87 SS.reconfigure forSampling @05:35:09.91 SS.seek 25.00ml @05:35:10.90 Vacuum too high -- waiting up to 40s @05:35:12.01 Intake @48.4psia, vacuum from 20.0 to 24.0psi @05:35:12.03 SS.reconfigure forSampling @05:35:12.06 SS.seek 25.00ml @05:35:13.71 Vacuum too high -- waiting up to 40s @05:35:14.42 Intake @49.2psia, vacuum from 20.0 to 24.0psi @05:35:14.44 SS.reconfigure forSampling @05:35:14.47 SS.seek 25.00ml @05:35:15.46 Vacuum too high -- waiting up to 40s @05:35:16.18 Intake @49.1psia, vacuum from 20.0 to 24.0psi @05:35:16.19 SS.reconfigure forSampling @05:35:16.23 SS.seek 25.00ml @05:35:17.40 Vacuum too high -- waiting up to 40s @05:35:18.11 Intake @49.4psia, vacuum from 20.0 to 24.0psi @05:35:18.13 SS.reconfigure forSampling @05:35:18.16 SS.seek 25.00ml @05:35:19.18 Vacuum too high -- waiting up to 40s @05:35:19.89 Intake @49.3psia, vacuum from 20.0 to 24.0psi @05:35:19.91 SS.reconfigure forSampling @05:35:19.94 SS.seek 25.00ml @05:35:20.99 Vacuum too high -- waiting up to 40s @05:35:21.70 Intake @49.0psia, vacuum from 20.0 to 24.0psi @05:35:21.71 SS.reconfigure forSampling @05:35:21.75 SS.seek 25.00ml @05:35:22.84 Vacuum too high -- waiting up to 40s @05:35:23.55 Intake @48.8psia, vacuum from 20.0 to 24.0psi @05:35:23.57 SS.reconfigure forSampling @05:35:23.60 SS.seek 25.00ml @05:35:24.84 Vacuum too high -- waiting up to 40s @05:35:25.55 Intake @49.0psia, vacuum from 20.0 to 24.0psi @05:35:25.57 SS.reconfigure forSampling @05:35:25.60 SS.seek 25.00ml @05:35:26.81 Vacuum too high -- waiting up to 40s @05:35:27.51 Intake @50.0psia, vacuum from 20.0 to 24.0psi @05:35:27.52 SS.reconfigure forSampling @05:35:27.56 SS.seek 25.00ml @05:35:28.46 Vacuum too high -- waiting up to 40s @05:35:28.94 Intake @49.1psia, vacuum from 20.0 to 24.0psi @05:35:28.96 SS.reconfigure forSampling @05:35:28.99 SS.seek 25.00ml @05:35:29.96 Vacuum too high -- waiting up to 40s @05:35:30.45 Intake @48.6psia, vacuum from 20.0 to 24.0psi @05:35:30.47 SS.reconfigure forSampling @05:35:30.50 SS.seek 25.00ml @05:35:31.46 Vacuum too high -- waiting up to 40s @05:35:32.16 Intake @49.0psia, vacuum from 20.0 to 24.0psi @05:35:32.17 SS.reconfigure forSampling @05:35:32.21 SS.seek 25.00ml @05:35:33.30 Vacuum too high -- waiting up to 40s @05:35:34.02 SS.seek 25.00ml @05:35:35.02 Vacuum too high -- waiting up to 40s @05:35:35.72 Intake @48.9psia, vacuum from 20.0 to 24.0psi @05:35:35.74 SS.reconfigure forSampling @05:35:35.77 SS.seek 25.00ml @05:35:36.87 Vacuum too high -- waiting up to 40s @05:35:37.57 Intake @48.9psia, vacuum from 20.0 to 24.0psi @05:35:37.58 SS.reconfigure forSampling @05:35:37.62 SS.seek 25.00ml @05:35:38.77 Vacuum too high -- waiting up to 40s @05:35:39.47 Intake @48.7psia, vacuum from 20.0 to 24.0psi @05:35:39.48 SS.reconfigure forSampling @05:35:40.14 SS.seek 25.00ml @05:35:41.87 Vacuum too high -- waiting up to 40s @05:35:42.58 Intake @49.3psia, vacuum from 20.0 to 24.0psi @05:35:42.60 SS.reconfigure forSampling @05:35:42.63 SS.seek 25.00ml @05:35:43.62 Vacuum too high -- waiting up to 40s @05:35:44.33 Intake @49.2psia, vacuum from 20.0 to 24.0psi @05:35:44.35 SS.reconfigure forSampling @05:35:44.38 SS.seek 25.00ml @05:35:45.46 Vacuum too high -- waiting up to 40s @05:35:46.18 Intake @49.0psia, vacuum from 20.0 to 24.0psi @05:35:46.19 SS.reconfigure forSampling @05:35:46.23 SS.seek 25.00ml @05:35:47.34 Vacuum too high -- waiting up to 40s @05:35:48.05 Intake @48.6psia, vacuum from 20.0 to 24.0psi @05:35:48.07 SS.reconfigure forSampling @05:35:48.10 SS.seek 25.00ml @05:35:49.21 Vacuum too high -- waiting up to 40s @05:35:49.92 Intake @48.7psia, vacuum from 20.0 to 24.0psi @05:35:49.93 SS.reconfigure forSampling @05:35:49.97 SS.seek 25.00ml @05:35:50.99 Vacuum too high -- waiting up to 40s @05:35:51.70 Intake @49.2psia, vacuum from 20.0 to 24.0psi @05:35:51.72 SS.reconfigure forSampling @05:35:51.75 SS.seek 25.00ml @05:35:52.77 Vacuum too high -- waiting up to 40s @05:35:53.48 Intake @48.8psia, vacuum from 20.0 to 24.0psi @05:35:53.50 SS.reconfigure forSampling @05:35:53.53 SS.seek 25.00ml @05:35:54.65 Vacuum too high -- waiting up to 40s @05:35:55.36 Intake @48.7psia, vacuum from 20.0 to 24.0psi @05:35:55.38 SS.reconfigure forSampling @05:35:55.41 SS.seek 25.00ml @05:35:56.49 Vacuum too high -- waiting up to 40s @05:35:57.18 Intake @49.4psia, vacuum from 20.0 to 24.0psi @05:35:57.20 SS.reconfigure forSampling @05:35:57.23 SS.seek 25.00ml @05:35:58.15 Vacuum too high -- waiting up to 40s @05:35:58.86 Intake @49.0psia, vacuum from 20.0 to 24.0psi @05:35:58.88 SS.reconfigure forSampling @05:35:58.91 SS.seek 25.00ml @05:36:00.02 Vacuum too high -- waiting up to 40s @05:36:00.73 Intake @49.0psia, vacuum from 20.0 to 24.0psi @05:36:00.74 SS.reconfigure forSampling @05:36:00.77 SS.seek 25.00ml @05:36:01.70 delay 1:00 @05:36:01.83 Vacuum too high -- waiting up to 40s @05:36:02.54 Intake @49.3psia, vacuum from 20.0 to 24.0psi @05:36:02.55 SS.reconfigure forSampling @05:36:02.58 SS.seek 25.00ml @05:36:03.55 Vacuum too high -- waiting up to 40s @05:36:04.26 SS.seek 25.00ml @05:36:05.30 Vacuum too high -- waiting up to 40s @05:36:06.01 Intake @49.2psia, vacuum from 20.0 to 24.0psi @05:36:06.03 SS.reconfigure forSampling @05:36:06.06 SS.seek 25.00ml @05:36:07.15 Vacuum too high -- waiting up to 40s @05:36:07.84 Intake @49.3psia, vacuum from 20.0 to 24.0psi @05:36:07.85 SS.reconfigure forSampling @05:36:08.52 SS.seek 25.00ml @05:36:09.87 Vacuum too high -- waiting up to 40s @05:36:10.58 Intake @49.2psia, vacuum from 20.0 to 24.0psi @05:36:10.60 SS.reconfigure forSampling @05:36:10.63 SS.seek 25.00ml @05:36:11.62 Vacuum too high -- waiting up to 40s @05:36:12.34 SS.seek 25.00ml @05:36:13.37 Vacuum too high -- waiting up to 40s @05:36:14.08 Intake @49.4psia, vacuum from 20.0 to 24.0psi @05:36:14.10 SS.reconfigure forSampling @05:36:14.13 SS.seek 25.00ml @05:36:15.18 Vacuum too high -- waiting up to 40s @05:36:15.89 Intake @49.3psia, vacuum from 20.0 to 24.0psi @05:36:15.91 SS.reconfigure forSampling @05:36:15.94 SS.seek 25.00ml @05:36:16.93 Vacuum too high -- waiting up to 40s @05:36:17.64 Intake @49.0psia, vacuum from 20.0 to 24.0psi @05:36:17.66 SS.reconfigure forSampling @05:36:17.69 SS.seek 25.00ml @05:36:18.65 Vacuum too high -- waiting up to 40s @05:36:19.36 Intake @48.7psia, vacuum from 20.0 to 24.0psi @05:36:19.38 SS.reconfigure forSampling @05:36:19.41 SS.seek 25.00ml @05:36:20.46 Vacuum too high -- waiting up to 40s @05:36:21.19 Intake @49.1psia, vacuum from 20.0 to 24.0psi @05:36:21.21 SS.reconfigure forSampling @05:36:21.25 SS.seek 25.00ml @05:36:22.36 Vacuum too high -- waiting up to 40s @05:36:23.07 Intake @49.4psia, vacuum from 20.0 to 24.0psi @05:36:23.09 SS.reconfigure forSampling @05:36:23.12 SS.seek 25.00ml @05:36:24.08 Vacuum too high -- waiting up to 40s @05:36:24.79 Intake @49.1psia, vacuum from 20.0 to 24.0psi @05:36:24.80 SS.reconfigure forSampling @05:36:24.84 SS.seek 25.00ml @05:36:25.80 Vacuum too high -- waiting up to 40s @05:36:26.52 Intake @49.0psia, vacuum from 20.0 to 24.0psi @05:36:26.54 SS.reconfigure forSampling @05:36:26.57 SS.seek 25.00ml @05:36:27.61 Vacuum too high -- waiting up to 40s @05:36:28.32 Intake @49.0psia, vacuum from 20.0 to 24.0psi @05:36:28.33 SS.reconfigure forSampling @05:36:28.37 SS.seek 25.00ml @05:36:29.43 Vacuum too high -- waiting up to 40s @05:36:30.14 Intake @49.2psia, vacuum from 20.0 to 24.0psi @05:36:30.15 SS.reconfigure forSampling @05:36:30.19 SS.seek 25.00ml @05:36:31.18 Vacuum too high -- waiting up to 40s @05:36:31.89 Intake @49.2psia, vacuum from 20.0 to 24.0psi @05:36:31.91 SS.reconfigure forSampling @05:36:31.94 SS.seek 25.00ml @05:36:32.90 Vacuum too high -- waiting up to 40s @05:36:33.61 Intake @48.9psia, vacuum from 20.0 to 24.0psi @05:36:33.63 SS.reconfigure forSampling @05:36:33.66 SS.seek 25.00ml @05:36:34.74 Vacuum too high -- waiting up to 40s @05:36:35.44 Intake @49.3psia, vacuum from 20.0 to 24.0psi @05:36:35.45 SS.reconfigure forSampling @05:36:35.49 SS.seek 25.00ml @05:36:37.55 Vacuum too high -- waiting up to 40s @05:36:38.27 Intake @49.2psia, vacuum from 20.0 to 24.0psi @05:36:38.28 SS.reconfigure forSampling @05:36:38.31 SS.seek 25.00ml @05:36:39.27 Vacuum too high -- waiting up to 40s @05:36:39.98 Intake @48.8psia, vacuum from 20.0 to 24.0psi @05:36:40.00 SS.reconfigure forSampling @05:36:40.03 SS.seek 25.00ml @05:36:41.02 Vacuum too high -- waiting up to 40s @05:36:41.73 Intake @49.3psia, vacuum from 20.0 to 24.0psi @05:36:41.75 SS.reconfigure forSampling @05:36:41.78 SS.seek 25.00ml @05:36:42.77 Vacuum too high -- waiting up to 40s @05:36:43.49 SS.seek 25.00ml @05:36:44.43 Vacuum too high -- waiting up to 40s @05:36:45.14 Intake @49.1psia, vacuum from 20.0 to 24.0psi @05:36:45.16 SS.reconfigure forSampling @05:36:45.19 SS.seek 25.00ml @05:36:46.21 Vacuum too high -- waiting up to 40s @05:36:46.92 Intake @49.3psia, vacuum from 20.0 to 24.0psi @05:36:46.94 SS.reconfigure forSampling @05:36:46.97 SS.seek 25.00ml @05:36:47.86 Vacuum too high -- waiting up to 40s @05:36:48.57 Intake @49.1psia, vacuum from 20.0 to 24.0psi @05:36:48.59 SS.reconfigure forSampling @05:36:48.62 SS.seek 25.00ml @05:36:49.64 Vacuum too high -- waiting up to 40s @05:36:50.35 Intake @49.4psia, vacuum from 20.0 to 24.0psi @05:36:50.37 SS.reconfigure forSampling @05:36:50.40 SS.seek 25.00ml @05:36:51.39 Vacuum too high -- waiting up to 40s @05:36:52.09 Intake @49.2psia, vacuum from 20.0 to 24.0psi @05:36:52.11 SS.reconfigure forSampling @05:36:52.14 SS.seek 25.00ml @05:36:53.11 Vacuum too high -- waiting up to 40s @05:36:53.81 Intake @49.4psia, vacuum from 20.0 to 24.0psi @05:36:53.83 SS.reconfigure forSampling @05:36:53.86 SS.seek 25.00ml @05:36:54.93 Vacuum too high -- waiting up to 40s @05:36:55.64 Intake @49.7psia, vacuum from 20.0 to 24.0psi @05:36:55.66 SS.reconfigure forSampling @05:36:55.69 SS.seek 25.00ml @05:36:56.64 Vacuum too high -- waiting up to 40s @05:36:57.34 Intake @49.0psia, vacuum from 20.0 to 24.0psi @05:36:57.36 SS.reconfigure forSampling @05:36:57.39 SS.seek 25.00ml @05:36:58.46 Vacuum too high -- waiting up to 40s @05:36:59.16 Intake @48.9psia, vacuum from 20.0 to 24.0psi @05:36:59.18 SS.reconfigure forSampling @05:36:59.21 SS.seek 25.00ml @05:37:00.27 Vacuum too high -- waiting up to 40s @05:37:00.98 Intake @48.7psia, vacuum from 20.0 to 24.0psi @05:37:01.00 SS.reconfigure forSampling @05:37:01.03 SS.seek 25.00ml @05:37:01.71 AFV.select :carrierSolution @05:37:01.80 delay 2 seconds @05:37:02.05 Vacuum too high -- waiting up to 40s @05:37:02.76 Intake @49.0psia, vacuum from 20.0 to 24.0psi @05:37:02.78 SS.reconfigure forSampling @05:37:02.81 SS.seek 25.00ml @05:37:03.81 AS.reconfigure ASconfig @05:37:03.85 AS.seek 1.000ml @05:37:04.83 Vacuum too high -- waiting up to 40s @05:37:05.54 SS.reconfigure SSconfig @05:37:05.58 SS.hold @05:37:05.67 Intake.select :close @05:37:05.82 ShallowSampler::Clogged in WCR -- progress too slow after 272.1ml @05:37:05.83 SSV.dial :exhaust @05:37:06.62 Exhaust.select :open @05:37:06.72 SS.reconfigure SSexhale @05:37:06.76 SS.seek 0.00ml @05:37:14.84 Exhaust.select :close @05:37:14.95 Sampled 272.1ml @05:37:14.96 collectSample: Complete @05:37:14.97 Drying puck before fixing for 04:00.00 @05:37:14.98 CBV.dial :waste2b @05:37:15.76 delay 1:00 @05:37:17.94 Sleepy.power :sampler,:OFF @05:38:02.45 delay 1 second @05:38:03.46 AFV.select :holdingCoil @05:38:03.55 delay 1 second @05:38:04.56 ATV.dial :ASV @05:38:04.57 ASV.dial :wasteR @05:38:04.58 AS.reconfigure ASslow1 @05:38:04.61 AS.seek 0.000ml @05:38:15.76 CSV.dial :air @05:38:16.19 CS.reconfigure CSfast @05:38:16.22 CS.seek 10.000ml @05:38:40.15 CBV.dial between CSV and waste2b @05:38:40.46 Pumping wcr puck from tube 3 up to 55psi... @05:38:40.51 CBV.dial SSV @05:38:41.94 CBV.dial between CSV and waste2b @05:38:42.89 CSV.dial between air and CTV @05:38:43.29 CS.seek 6.657ml @05:38:51.69 CTV.dial CSV,avoiding => air @05:38:51.71 CSV.dial CTV @05:38:52.46 CS.reconfigure CSpressurize @05:38:52.49 CS.seek 0.000ml @05:39:09.87 CSV.dial :air @05:39:10.39 CS.reconfigure CSfast @05:39:10.42 CS.seek 10.000ml @05:39:34.14 CSV.dial between air and CTV @05:39:34.52 CS.seek 2.100ml @05:39:53.25 CTV.dial CSV,avoiding => air @05:39:53.26 CSV.dial CTV @05:39:53.55 CS.reconfigure CSpressurize @05:39:53.58 CS.seek 0.000ml @05:39:54.54 CBV.dial :waste2b @05:39:54.86 CS.reconfigure CSconfig @05:39:54.89 CS.seek empty @05:40:00.52 delay 1:00 @05:41:00.53 CTV.dial :air,avoiding => intake @05:41:01.83 Fix #1 of 2 (with rnal) for 02:00.00 @05:41:01.84 CS Pulling 2ml of rnal CSV.dial CSR @05:41:02.27 CSR.select :rnal @05:41:02.37 CS.seek 2.000ml @05:41:08.30 delay 5 seconds @05:41:13.31 CSR.select :air @05:41:13.40 CS.reconfigure CSfast @05:41:13.44 CS.seek 10.000ml @05:41:28.87 delay 20 seconds @05:41:32.58 CBV.dial between CSV and waste2b @05:41:32.90 Pumping wcr puck from tube 3 up to 55psi... @05:41:32.94 CBV.dial SSV @05:41:34.37 CBV.dial between CSV and waste2b @05:41:35.32 CSV.dial between air and CTV @05:41:35.97 CS.seek 6.588ml @05:41:44.59 CTV.dial CSV,avoiding => air @05:41:44.61 CSV.dial CTV @05:41:45.92 CS.reconfigure CSpressurize @05:41:45.95 CS.seek 0.000ml @05:41:48.88 ATV.dial :air3 @05:41:50.01 AS.seek 0.590ml @05:42:02.26 delay 2:00 @05:43:50.64 delay 3 seconds @05:43:53.65 ATV.dial :ASV @05:43:54.80 ASV.dial :wasteR @05:43:54.86 AS.seek 0.090ml @05:44:02.27 CBV.dial :waste2b @05:44:02.60 CS.reconfigure CSconfig @05:44:02.63 CS.seek empty @05:44:04.29 delay 1:00 @05:45:04.30 CTV.dial :air,avoiding => intake @05:45:05.58 Fix #2 of 2 (with rnal) for 02:00.00 @05:45:05.59 CS Pulling 2ml of rnal CSV.dial CSR @05:45:06.01 CSR.select :rnal @05:45:06.11 CS.seek 2.000ml @05:45:12.07 delay 5 seconds @05:45:17.08 CSR.select :air @05:45:17.72 CS.reconfigure CSfast @05:45:17.76 CS.seek 10.000ml @05:45:36.88 CBV.dial between CSV and waste2b @05:45:37.08 delay 30 seconds @05:45:37.21 Pumping wcr puck from tube 3 up to 55psi... @05:45:37.26 CBV.dial SSV @05:45:38.69 CBV.dial between CSV and waste2b @05:45:39.64 CSV.dial between air and CTV @05:45:40.30 CS.seek 6.510ml @05:45:49.17 CTV.dial CSV,avoiding => air @05:45:49.19 CSV.dial CTV @05:45:50.50 CS.reconfigure CSpressurize @05:45:50.53 CS.seek 0.000ml @05:46:06.25 delay 2:00 @05:46:07.09 ATV.dial :extSrc @05:46:07.61 delay 3 seconds @05:46:10.62 ATV.dial :ElutionWater @05:46:11.55 AS.seek 0.120ml @05:46:17.92 ATV.dial :air3 @05:46:18.30 AS.seek 0.130ml @05:46:20.59 delay 2 seconds @05:46:22.60 ATV.dial :bleach @05:46:22.98 AS.seek 0.230ml @05:46:43.65 delay 3 seconds @05:46:46.66 ATV.dial :air3 @05:46:47.00 delay 3 seconds @05:46:50.06 AS.seek 0.255ml @05:46:55.40 delay 3 seconds @05:46:58.41 ATV.dial :bleach @05:46:58.80 AS.seek 0.240ml @05:47:02.04 delay 3 seconds @05:47:05.05 ATV.dial :ASV @05:47:05.93 ASV.dial :SPEdelivery @05:47:06.40 AFV.select :carrierSolution @05:47:06.50 delay 2 seconds @05:47:08.51 AS.reconfigure ASconfig @05:47:08.54 AS.seek 0.500ml @05:47:23.99 delay 1 second @05:47:25.00 AFV.select :holdingCoil @05:47:25.14 AS.reconfigure ASslow1 @05:47:25.17 AS.seek 0.170ml @05:48:06.26 CBV.dial :waste2b @05:48:06.59 CS.reconfigure CSconfig @05:48:06.62 CS.seek empty @05:48:08.63 delay 1:00 @05:48:32.68 SPE.hold 2:46:40,95.0C @05:48:32.71 delay 3:00 @05:49:08.64 CTV.dial :air,avoiding => intake @05:49:10.00 Storing wcr puck in Collection Clamp to tube 2 @05:49:10.13 SC.reconfigure SCtight @05:49:10.16 SC.seek 2 @05:49:12.41 SC.reconfigure SCloose @05:49:12.59 Elbow.reconfigure TightElbow @05:49:12.62 Elbow.seek 2 @05:49:14.24 Forearm.reconfigure TightForearm @05:49:14.27 Forearm.seek 2 + 800 counts @05:49:15.38 SE.reconfigure SEpick @05:49:15.43 SE.seek beamBreak @05:49:17.25 SE.configure SEconfig @05:49:17.37 Elbow.reconfigure LimpElbow @05:49:17.41 Forearm.reconfigure LooseForearm @05:49:17.45 Forearm.seek 2 @05:49:17.65 Elbow.hold @05:49:17.70 Elbow.reconfigure TightElbow @05:49:17.78 SE.seek 12.03 Pucks below Top Plate @05:49:18.19 Forearm.coast @05:49:18.22 Forearm.seek 2 + 334 counts @05:49:18.50 Forearm.reconfigure TightForearm @05:49:18.53 Forearm.seek 2 + 800 counts @05:49:18.90 SE.seek 13.03 Pucks below Top Plate @05:49:19.58 Forearm.reconfigure LooseForearm @05:49:19.61 Forearm.seek retract @05:49:20.55 Elbow.seek CC @05:49:22.08 Elbow.reconfigure LooseElbow @05:49:22.16 Forearm.seek CC @05:49:23.15 CC.seek sealed @05:49:24.09 Hand.grip @05:49:24.80 CC.seek open @05:49:28.69 Forearm.seek retract @05:49:29.69 Elbow.reconfigure TightElbow @05:49:29.72 Elbow.seek 2 @05:49:31.33 Elbow.reconfigure LimpElbow @05:49:31.37 Forearm.seek 2 @05:49:32.43 Elbow.hold @05:49:32.48 Elbow.reconfigure TightElbow @05:49:32.52 Hand.release @05:49:33.38 Forearm.coast @05:49:33.42 Forearm.seek 2 + 310 counts @05:49:33.66 Forearm.reconfigure TightForearm @05:49:33.69 Forearm.seek 2 + 800 counts @05:49:34.07 SE.seek down @05:49:35.17 Forearm.reconfigure LooseForearm @05:49:35.20 Forearm.seek retract @05:49:35.52 SE.coast @05:49:36.17 SC.coast @05:49:36.21 Starting finalPurge Loading FlushPuck into Collection Clamp from FlushPuck::Garage @05:49:36.37 Elbow.seek FlushPuck::Garage @05:49:38.13 Elbow.reconfigure LooseElbow @05:49:38.21 Forearm.seek garage @05:49:39.26 Hand.grip @05:49:40.14 Forearm.seek retract @05:49:41.19 Elbow.reconfigure TightElbow @05:49:41.22 Elbow.seek CC @05:49:43.98 Elbow.reconfigure LooseElbow @05:49:44.06 Forearm.reconfigure TightForearm @05:49:44.09 Forearm.seek CC @05:49:45.19 CC.seek closed @05:49:48.90 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 2.18 @05:49:48.95 Hand.release @05:49:49.72 Forearm.reconfigure LooseForearm @05:49:49.75 Forearm.seek retract @05:49:50.75 Flushing the Collection Valve::Manifold @05:49:50.85 CS Pulling 5ml of flush CSV.dial CSR @05:49:51.28 CSR.select :flush @05:49:51.39 CS.seek 5.000ml @05:50:04.84 delay 5 seconds @05:50:09.85 CSR.select :air @05:50:09.99 CS.reconfigure CSfast @05:50:10.02 CS.seek 7.499ml @05:50:16.84 CSV.dial :waste @05:50:18.16 CS.reconfigure CSconfig @05:50:18.19 CS.seek 0.000ml @05:50:37.94 Flushing lower manifold's waste1 port @05:50:38.03 CSV.dial :flush @05:50:38.48 CS.reconfigure CSfast @05:50:38.51 CS.seek 5.000ml @05:50:51.25 delay 5 seconds @05:50:56.26 CSV.dial :air @05:50:56.74 CS.seek 7.000ml @05:51:02.29 CSV.dial CTV @05:51:02.31
CTV.dial CSV @05:51:03.68 CBV.dial :waste1 @05:51:04.47 CS.seek 0.000ml @05:51:21.72 Flushing lower manifold's waste2b port @05:51:21.81 CSV.dial :flush @05:51:23.25 CS.seek 5.000ml @05:51:32.74 AFV.select :carrierSolution @05:51:32.83 delay 2 seconds @05:51:34.84 AS.reconfigure ASconfig @05:51:34.87 AS.seek 1.000ml @05:51:35.85
delay 5 seconds @05:51:40.86 CSV.dial :air @05:51:41.43 CS.seek 7.000ml @05:51:46.94 CSV.dial CTV @05:51:46.96
CTV.dial CSV @05:51:47.42 CBV.dial :waste2b @05:51:48.20 CS.seek 0.000ml @05:52:05.19 Flushing upper manifold @05:52:05.28 CSV.dial :flush @05:52:06.11 CS.seek 5.000ml @05:52:18.66 delay 5 seconds @05:52:23.57 delay 1 second @05:52:23.67
CSV.dial :air @05:52:24.24 CS.seek 7.000ml @05:52:24.58 AFV.select :holdingCoil @05:52:24.67 delay 1 second @05:52:25.68 ASV.dial :wasteD @05:52:26.97 AS.seek 0.000ml @05:52:29.73 CSV.dial CBV @05:52:29.74
CBV.dial CSV @05:52:30.89 CTV.dial :air @05:52:32.18 CS.seek 0.000ml @05:52:49.00 delay 7 seconds @05:52:56.01 CS.seek 3.000ml @05:53:03.81 CSV.dial :waste @05:53:04.54 CS.seek 0.000ml @05:53:12.31 CSV.dial :flush @05:53:12.84 CS.seek 3.000ml @05:53:20.62 delay 5 seconds @05:53:25.57 Priming Eluate @05:53:25.58 AFV.select :carrierSolution @05:53:25.63
CSV.dial :air @05:53:25.67 delay 2 seconds @05:53:26.12
CS.seek 5.000ml @05:53:27.68 AS.seek 0.500ml @05:53:31.56
CSV.dial :air @05:53:31.58 CS.seek 0.000ml @05:53:43.78 delay 7 seconds @05:53:50.79 CS.seek 3.000ml @05:53:57.12 delay 1 second @05:53:58.13 AFV.select :holdingCoil @05:53:58.22 ATV.dial ASV @05:53:58.23 ASV.dial :SPErecovery @05:53:58.50
CSV.dial :waste @05:53:58.73 Swish #1 of 2 @05:53:58.78 AS.reconfigure ASslow0 @05:53:58.82 AS.seek 0.600ml @05:53:59.30
CS.seek 0.000ml @05:54:07.00 Disinfecting external Intake with mfbkill @05:54:07.09 CSV.dial CSR @05:54:08.37 CSR.select :mfbkill @05:54:08.47 CS.seek 8.000ml @05:54:09.39 AS.seek 0.500ml @05:54:19.90 Swish #2 of 2 @05:54:19.96 AS.seek 0.600ml @05:54:27.65
delay 5 seconds @05:54:30.54 AS.seek 0.500ml @05:54:32.66
CSR.select :air @05:54:32.75 CS.seek 10.000ml @05:54:38.15 CSV.dial CBV @05:54:38.17
CBV.dial CSV @05:54:39.25 CTV.dial :intake @05:54:39.95 Intake.select :open @05:54:40.04 CS.seek 2.000ml @05:54:41.04 delay 3 seconds @05:54:44.10 AS.reconfigure ASslow1 @05:54:44.13 AS.seek 0.676ml @05:54:59.31
Intake.select :close @05:54:59.40 CTV.dial :air @05:55:00.15 CS.reconfigure CSconfig @05:55:00.19 CS.seek 0.000ml @05:55:06.02 Flushing lower manifold's waste1 port @05:55:06.03 CS Pulling 4ml of mfbkill @05:55:06.04 CSV.dial CSR @05:55:07.10 CSR.select :mfbkill @05:55:07.20 CS.seek 4.000ml @05:55:18.21 delay 5 seconds @05:55:20.30 delay 3 seconds @05:55:23.22
CSR.select :air @05:55:23.31 CS.reconfigure CSfast @05:55:23.34 Eluate primed @05:55:23.35 ATV.dial :waste2 @05:55:23.37
CS.seek 10.000ml @05:55:23.91 AS.reconfigure ASnorm @05:55:23.94 AS.seek 0.000ml @05:55:37.90 CSV.dial CTV @05:55:37.92
CTV.dial CSV @05:55:39.27 CBV.dial :waste1 @05:55:39.74 CS.seek 0.000ml @05:55:58.80 SPE.coast @05:55:58.88 ASV.dial :SPErecovery @05:55:58.90 Setting up PCR for HF183em -- writing /var/log/esp/HF183em14mar1505h.pcr @05:55:58.91 Building PCR Slug from ASV with MM2 mix, PP4 primer Priming with carrier @05:55:58.92 ATV.dial ARV @05:55:59.63 ARV.dial :carrier @05:56:00.50 AS.seek 0.500ml @05:56:03.24
Flushing lower manifold's waste2b port @05:56:03.25 CS Pulling 4ml of mfbkill @05:56:03.26 CSV.dial CSR @05:56:03.76 CSR.select :mfbkill @05:56:03.86 CS.reconfigure CSconfig @05:56:03.90 CS.seek 4.000ml @05:56:14.90 delay 5 seconds @05:56:19.91 CSR.select :air @05:56:20.00 CS.reconfigure CSfast @05:56:20.04 CS.seek 10.000ml @05:56:26.43 AFV.select :carrierSolution @05:56:26.54 delay 2 seconds @05:56:28.55 AS.reconfigure ASconfig @05:56:28.59 AS.seek 1.000ml @05:56:34.47 CSV.dial CTV @05:56:34.49
CTV.dial CSV @05:56:35.00 CBV.dial :waste2b @05:56:35.73 CS.seek 0.000ml @05:56:58.02 delay 1 second @05:56:58.80
Killing upper manifold @05:56:58.81 CS Pulling 4ml of mfbkill @05:56:58.82 CSV.dial CSR @05:56:59.03 AFV.select :holdingCoil @05:56:59.13 delay 1 second @05:56:59.32
CSR.select :mfbkill @05:56:59.42 CS.reconfigure CSconfig @05:56:59.46 CS.seek 4.000ml @05:57:00.14 ATV.dial :waste @05:57:01.40 AS.reconfigure ASnorm @05:57:01.44 AS.seek empty @05:57:10.36
delay 5 seconds @05:57:15.37 CSR.select :air @05:57:15.46 CS.reconfigure CSfast @05:57:15.50 CS.seek 10.000ml @05:57:29.89 CSV.dial CBV @05:57:29.91
CBV.dial CSV @05:57:30.95 CTV.dial :air @05:57:32.22 CS.seek 0.000ml @05:57:52.92 AFV.select :carrierSolution @05:57:53.02 delay 2 seconds @05:57:55.03 AS.reconfigure ASconfig @05:57:55.06 AS.seek 0.500ml @05:57:55.33
delay 7 seconds @05:58:02.34 CS.seek 3.000ml @05:58:09.89 CSV.dial :waste @05:58:10.32 CS.seek 0.000ml @05:58:17.83 Flushing upper manifold @05:58:17.92 CSV.dial :flush @05:58:18.35 CS.seek 5.000ml @05:58:24.55 delay 1 second @05:58:25.56 AFV.select :holdingCoil @05:58:25.65 Building Slug @05:58:25.66 ATV.dial :ARV @05:58:26.91 delay 2 seconds @05:58:28.92 ARV.dial :air1 @05:58:29.25 delay 2 seconds @05:58:30.42
delay 5 seconds @05:58:31.31 AS.reconfigure ASslow2 @05:58:31.34 AS.seek 0.506ml @05:58:35.43
CSV.dial :air @05:58:35.91 CS.seek 7.000ml @05:58:37.81 delay 2 seconds @05:58:39.82 ARV.dial :MM2 @05:58:41.05 delay 2 seconds @05:58:41.23 CSV.dial CBV @05:58:41.26
CBV.dial CSV @05:58:42.34 CTV.dial :air @05:58:42.35 CS.seek 0.000ml @05:58:43.06 Adding MM2 @05:58:43.12 AS.seek 0.524ml @05:58:58.61
delay 7 seconds @05:59:01.83 delay 25 seconds @05:59:05.62
CS.seek 3.000ml @05:59:13.17 CSV.dial :waste @05:59:13.68 CS.seek 0.000ml @05:59:21.31 CSV.dial :flush @05:59:21.74 CS.seek 3.000ml @05:59:26.84 ARV.dial :PP4 @05:59:27.18 Adding PP4 @05:59:27.19 delay 2 seconds @05:59:29.24 AS.seek 0.530ml @05:59:29.29
delay 5 seconds @05:59:34.30 CSV.dial :air @05:59:34.77 CS.seek 5.001ml @05:59:35.65 delay 10 seconds @05:59:40.14
CSV.dial :air @05:59:40.16 CS.seek 0.000ml @05:59:45.66 ARV.dial :air3 @05:59:45.99 adding ARV air @05:59:46.00 delay 2 seconds @05:59:48.05 AS.seek 0.536ml @05:59:52.11
delay 7 seconds @05:59:54.36 delay 5 seconds @05:59:59.12
CS.seek 3.000ml @05:59:59.37 ARV.dial :carrier @06:00:00.41 delay 7 seconds @06:00:06.67
CSV.dial :waste @06:00:07.47 AS.seek 0.594ml @06:00:07.52
CS.seek 0.000ml @06:00:15.07 Flushing lower manifold's waste1 port @06:00:15.16 CSV.dial :flush @06:00:15.68 CS.seek 5.000ml @06:00:28.17 delay 5 seconds @06:00:33.18 CSV.dial :air @06:00:33.65 CS.seek 7.000ml @06:00:39.01 CSV.dial CTV @06:00:39.03
CTV.dial CSV @06:00:40.37 CBV.dial :waste1 @06:00:40.82 CS.seek 0.000ml @06:00:57.32 Flushing lower manifold's waste2b port @06:00:57.41 CSV.dial :flush @06:00:58.22 CS.seek 5.000ml @06:01:06.76 delay 5 seconds @06:01:10.29
delay 5 seconds @06:01:11.77 ATV.dial :ASV @06:01:12.11 delay 2 seconds @06:01:14.16 AS.seek 0.600ml @06:01:15.30
CSV.dial :air @06:01:15.86 CS.seek 7.000ml @06:01:20.43 delay 4 seconds @06:01:21.23 CSV.dial CTV @06:01:21.26
CTV.dial CSV @06:01:21.71 CBV.dial :waste2b @06:01:22.46 CS.seek 0.000ml @06:01:24.44 ATV.dial ARV @06:01:24.79 delay 2 seconds @06:01:26.79 ARV.dial :carrier @06:01:26.80 delay 2 seconds @06:01:28.85 AS.seek 0.650ml @06:01:38.76
Disinfecting external Intake with kill @06:01:38.85 CSV.dial CSR @06:01:39.27 CSR.select :kill @06:01:39.37 CS.seek 8.000ml @06:01:58.07 delay 5 seconds @06:02:03.08 CSR.select :air @06:02:03.17 CS.seek 10.000ml @06:02:08.54 CSV.dial CBV @06:02:08.56
CBV.dial CSV @06:02:09.58 CTV.dial :intake @06:02:10.29 Intake.select :open @06:02:10.39 CS.seek 2.000ml @06:02:20.02 delay 4 seconds @06:02:24.03 ATV.dial PCR @06:02:24.63 ASV.dial :waste1 @06:02:25.54 ARV.dial :air1 @06:02:25.88 Positioning Slug by 0.324ml @06:02:25.94 AS.seek 0.326ml @06:02:29.29
Intake.select :close @06:02:29.38 CTV.dial :air @06:02:30.10 CS.reconfigure CSconfig @06:02:30.14 CS.seek 0.000ml @06:02:36.07 CSV.dial CSR @06:02:37.11 CSR.select :flush @06:02:37.21 CS.seek 5.000ml @06:02:50.72 delay 5 seconds @06:02:55.73 CSR.select :air @06:02:55.82 CS.seek 10.000ml @06:03:09.35 CSV.dial CBV @06:03:10.40 CS.seek 0.000ml @06:03:36.53 Garaging FlushPuck in Collection Clamp @06:03:36.59 Elbow.reconfigure TightElbow @06:03:36.62 Elbow.seek CC @06:03:36.72 Elbow.reconfigure LooseElbow @06:03:36.80 Forearm.seek CC @06:03:37.75 CC.seek sealed @06:03:38.69 Hand.grip @06:03:39.40 CC.seek open @06:03:42.69 Forearm.seek retract @06:03:43.63 Elbow.reconfigure TightElbow @06:03:43.67 Elbow.seek garage @06:03:46.46 Elbow.reconfigure LooseElbow @06:03:46.54 Forearm.reconfigure TightForearm @06:03:46.57 Forearm.seek garage @06:03:47.74 Hand.release @06:03:48.57 Forearm.reconfigure LooseForearm @06:03:48.60 Forearm.seek retract @06:03:49.63 Completed {coreNegNTCphase} begun 04:16:18 @06:03:49.65 deferred until MFBPCR finish @06:03:49.66
Waiting for MFBPCR til 8AM 3/17/14 @06:03:49.69 Sleeping until 08:00PDT17-Mar-14 @06:03:49.70 GoodNews.email "Then, in tube 3: wqPCR\n/home/esp/esp2/mission/14mar14sccwrp.rb:63",Subject => "Waiting until 08:00PDT17-Mar-14" @06:07:56.57 delay 5 seconds @06:08:01.58 ATV.dial ARV @06:08:02.19 ASV.dial :PCRlock @06:08:02.79 delay 5 seconds @06:08:07.80 Running HF183em ProfileEM60C2 thermocycling @06:08:07.81 PCR.program ProfileEM60C2 @06:08:07.92 ProfileEM60C2 should complete in < 2:11:38 @06:08:07.93 PCR.run 2:49:32.5 @08:01:51.41 PCR.off @08:01:51.93 PCR for HF183em completed -- closed /var/log/esp/HF183em14mar1505h.pcr @08:01:51.98 Cleaning PCR @08:01:51.99 AFV.select :carrierSolution @08:01:52.08 delay 2 seconds @08:01:54.09 AS.reconfigure ASconfig @08:01:54.12 AS.seek 0.500ml @08:02:04.53 delay 1 second @08:02:05.54 AFV.select :holdingCoil @08:02:05.63 ASV.dial :waste1 @08:02:06.04 ATV.dial :air3 @08:02:06.98 AS.reconfigure ASslow1 @08:02:07.02 AS.seek 0.510ml @08:02:09.33 delay 2 seconds @08:02:11.34 ATV.dial :bleach @08:02:11.74 AS.seek 0.810ml @08:03:13.18 delay 3 seconds @08:03:16.19 ATV.dial :air3 @08:03:16.53 delay 3 seconds @08:03:20.14 AS.seek 0.835ml @08:03:25.46 delay 3 seconds @08:03:28.47 ATV.dial :bleach @08:03:28.86 AS.seek 0.820ml @08:03:32.15 delay 3 seconds @08:03:35.16 ARV.dial :waste @08:03:35.65 delay 3 seconds @08:03:38.66 ATV.dial :ARV @08:03:39.48 AS.reconfigure ASconfig @08:03:39.51 AS.seek 0.470ml @08:04:00.21 AFV.select :carrierSolution @08:04:00.31 delay 2 seconds @08:04:02.32 AS.seek 1.000ml @08:04:33.52 delay 1 second @08:04:34.53 AFV.select :holdingCoil @08:04:34.62 delay 1 second @08:04:35.63 AS.reconfigure ASfast @08:04:35.67 AS.seek 0.000ml @08:05:03.41 AFV.select :carrierSolution @08:05:03.51 delay 2 seconds @08:05:05.52 AS.reconfigure ASconfig @08:05:05.55 AS.seek 1.000ml @08:06:04.15 delay 1 second @08:06:05.16 AFV.select :holdingCoil @08:06:05.25 delay 1 second @08:06:06.26 AS.reconfigure ASfast @08:06:06.30 AS.seek 0.000ml @08:06:34.01 AFV.select :carrierSolution @08:06:34.11 delay 2 seconds @08:06:36.12 AS.reconfigure ASconfig @08:06:36.15 AS.seek 1.000ml @08:07:34.79 delay 1 second @08:07:35.80 AFV.select :holdingCoil @08:07:35.89 delay 1 second @08:07:36.90 AS.reconfigure ASfast @08:07:36.93 AS.seek 0.000ml @08:08:04.65 ARV.dial between carrier and air1 @08:08:05.17 ATV.dial :air3 @08:08:06.11 AS.reconfigure ASslow1 @08:08:06.14 AS.seek 0.010ml @08:08:08.48 delay 2 seconds @08:08:10.49 ATV.dial :bleach @08:08:10.97 AS.seek 0.810ml @08:10:54.37 delay 3 seconds @08:10:57.38 ATV.dial :air3 @08:10:57.71 delay 3 seconds @08:11:00.77 AS.seek 0.835ml @08:11:06.10 delay 3 seconds @08:11:09.11 ATV.dial :bleach @08:11:09.59 AS.seek 0.820ml @08:11:12.90 delay 3 seconds @08:11:15.91 ATV.dial PCR @08:11:16.27 AS.reconfigure ASconfig @08:11:16.30 AS.seek empty @08:12:04.41 PCR.program :bleachProfile @08:12:04.46 PCR program should complete in < 2:45 @08:12:04.47 PCR.run 10:00 @08:15:05.68 PCR.off @08:15:05.71 AFV.select :carrierSolution @08:15:05.80 delay 2 seconds @08:15:07.81 AS.seek 1.000ml @08:16:06.45 delay 1 second @08:16:07.46 AFV.select :holdingCoil @08:16:07.55 delay 1 second @08:16:08.56 AS.reconfigure ASfast @08:16:08.59 AS.seek 0.000ml @08:16:36.33 delay 3 seconds @08:16:39.34 AFV.select :carrierSolution @08:16:39.43 delay 2 seconds @08:16:41.44 AS.reconfigure ASconfig @08:16:41.47 AS.seek 1.000ml @08:17:40.07 delay 1 second @08:17:41.08 AFV.select :holdingCoil @08:17:41.17 delay 1 second @08:17:42.18 AS.reconfigure ASfast @08:17:42.21 AS.seek 0.000ml @08:18:09.95 delay 3 seconds @08:18:12.96 AFV.select :carrierSolution @08:18:13.05 delay 2 seconds @08:18:15.06 AS.reconfigure ASconfig @08:18:15.09 AS.seek 1.000ml @08:19:13.66 delay 1 second @08:19:14.67 AFV.select :holdingCoil @08:19:14.76 delay 1 second @08:19:15.77 AS.reconfigure ASfast @08:19:15.80 AS.seek 0.000ml @08:19:43.52 delay 3 seconds @08:19:46.53 AFV.select :carrierSolution @08:19:46.62 delay 2 seconds @08:19:48.63 AS.reconfigure ASconfig @08:19:48.66 AS.seek 1.000ml @08:20:47.26 delay 1 second @08:20:48.27 AFV.select :holdingCoil @08:20:48.36 delay 1 second @08:20:49.37 AS.reconfigure ASfast @08:20:49.40 AS.seek 0.000ml @08:21:17.15 delay 3 seconds @08:21:20.16 AFV.select :carrierSolution @08:21:20.25 delay 2 seconds @08:21:22.26 AS.reconfigure ASconfig @08:21:22.29 AS.seek 1.000ml @08:22:20.91 delay 1 second @08:22:21.92 AFV.select :holdingCoil @08:22:22.01 delay 1 second @08:22:23.02 AS.reconfigure ASnorm @08:22:23.05 AS.seek 0.000ml @08:23:14.50 Setting up PCR for ENTap -- writing /var/log/esp/ENTap14mar1508h.pcr @08:23:14.51 Building PCR Slug from ASV with MM1 mix, PP1 primer @08:23:14.52 Priming with carrier @08:23:14.53 ATV.dial ARV @08:23:15.05 ARV.dial :carrier @08:23:15.32 AS.seek 0.500ml @08:23:41.22 AFV.select :carrierSolution @08:23:41.32 delay 2 seconds @08:23:43.33 AS.reconfigure ASconfig @08:23:43.36 AS.seek 1.000ml @08:24:12.79 delay 1 second @08:24:13.80 AFV.select :holdingCoil @08:24:13.89 delay 1 second @08:24:14.90 ATV.dial :waste @08:24:16.18 AS.reconfigure ASnorm @08:24:16.21 AS.seek empty @08:25:07.68 AFV.select :carrierSolution @08:25:07.77 delay 2 seconds @08:25:09.78 AS.reconfigure ASconfig @08:25:09.81 AS.seek 0.500ml @08:25:39.30 delay 1 second @08:25:40.31 AFV.select :holdingCoil @08:25:40.92 Building Slug @08:25:40.93 ATV.dial :ARV @08:25:42.27 delay 2 seconds @08:25:44.28 ARV.dial :air1 @08:25:44.60 delay 2 seconds @08:25:46.66 AS.reconfigure ASslow2 @08:25:46.69 AS.seek 0.506ml @08:25:52.93 delay 2 seconds @08:25:54.94 ARV.dial :MM1 @08:25:55.44 delay 2 seconds @08:25:57.45 Adding MM1 @08:25:57.51 AS.seek 0.524ml @08:26:16.04 delay 25 seconds @08:26:41.05 ARV.dial :PP1 @08:26:41.39 Adding PP1 @08:26:41.40 delay 2 seconds @08:26:43.45 AS.seek 0.530ml @08:26:49.74 delay 10 seconds @08:26:59.75 ARV.dial :air1 @08:27:00.08 adding ARV air @08:27:00.09 delay 2 seconds @08:27:02.14 AS.seek 0.536ml @08:27:08.57 delay 5 seconds @08:27:13.58 ARV.dial :carrier @08:27:13.89 delay 7 seconds @08:27:20.95 AS.seek 0.594ml @08:28:20.47 delay 5 seconds @08:28:25.48 ATV.dial :ASV @08:28:25.81 delay 2 seconds @08:28:27.87 AS.seek 0.600ml @08:28:34.33 delay 4 seconds @08:28:38.34 ATV.dial ARV @08:28:38.67 delay 2 seconds @08:28:40.68 ARV.dial :carrier @08:28:40.69 delay 2 seconds @08:28:42.74 AS.seek 0.650ml @08:29:33.97 delay 4 seconds @08:29:37.98 ATV.dial PCR @08:29:38.50 ASV.dial :waste1 @08:29:38.51 ARV.dial :air1 @08:29:38.85 Positioning Slug by 0.324ml @08:29:38.90 AS.seek 0.326ml @08:35:09.58 delay 5 seconds @08:35:14.59 ATV.dial ARV @08:35:15.11 ASV.dial :PCRlock @08:35:15.60 delay 5 seconds @08:35:20.61 Running ENTap Profile60C thermocycling @08:35:20.62 PCR.program Profile60C @08:35:20.73 Profile60C should complete in < 1:39:08 @08:35:20.74 PCR.run 2:08:55 @09:56:45.30 PCR.off @09:56:45.82 PCR for ENTap completed -- closed /var/log/esp/ENTap14mar1508h.pcr @09:56:45.87 Cleaning PCR @09:56:45.88 AFV.select :carrierSolution @09:56:45.97 delay 2 seconds @09:56:47.98 AS.reconfigure ASconfig @09:56:48.02 AS.seek 0.500ml @09:56:58.45 delay 1 second @09:56:59.46 AFV.select :holdingCoil @09:56:59.55 ASV.dial :waste1 @09:57:00.03 ATV.dial :air3 @09:57:01.05 AS.reconfigure ASslow1 @09:57:01.13 AS.seek 0.510ml @09:57:03.45 delay 2 seconds @09:57:05.46 ATV.dial :bleach @09:57:05.85 AS.seek 0.810ml @09:58:07.28 delay 3 seconds @09:58:10.29 ATV.dial :air3 @09:58:10.64 delay 3 seconds @09:58:13.70 AS.seek 0.835ml @09:58:19.04 delay 3 seconds @09:58:22.05 ATV.dial :bleach @09:58:22.44 AS.seek 0.820ml @09:58:25.76 delay 3 seconds @09:58:28.77 ARV.dial :waste @09:58:29.36 delay 3 seconds @09:58:32.37 ATV.dial :ARV @09:58:33.10 AS.reconfigure ASconfig @09:58:33.14 AS.seek 0.470ml @09:58:53.81 AFV.select :carrierSolution @09:58:53.91 delay 2 seconds @09:58:55.92 AS.seek 1.000ml @09:59:27.13 delay 1 second @09:59:28.14 AFV.select :holdingCoil @09:59:28.23 delay 1 second @09:59:29.24 AS.reconfigure ASfast @09:59:29.27 AS.seek 0.000ml @09:59:57.01 AFV.select :carrierSolution @09:59:57.11 delay 2 seconds @09:59:59.12 AS.reconfigure ASconfig @09:59:59.15 AS.seek 1.000ml @10:00:57.76 delay 1 second @10:00:58.77 AFV.select :holdingCoil @10:00:58.86 delay 1 second @10:00:59.87 AS.reconfigure ASfast @10:00:59.90 AS.seek 0.000ml @10:01:27.58 AFV.select :carrierSolution @10:01:27.68 delay 2 seconds @10:01:29.69 AS.reconfigure ASconfig @10:01:29.72 AS.seek 1.000ml @10:02:28.32 delay 1 second @10:02:29.33 AFV.select :holdingCoil @10:02:29.42 delay 1 second @10:02:30.43 AS.reconfigure ASfast @10:02:30.47 AS.seek 0.000ml @10:02:58.18 ARV.dial between carrier and air1 @10:02:58.60 ATV.dial :air3 @10:02:59.55 AS.reconfigure ASslow1 @10:02:59.58 AS.seek 0.010ml @10:03:01.93 delay 2 seconds @10:03:03.94 ATV.dial :bleach @10:03:04.33 AS.seek 0.810ml @10:05:47.72 delay 3 seconds @10:05:50.73 ATV.dial :air3 @10:05:51.08 delay 3 seconds @10:05:54.14 AS.seek 0.835ml @10:05:59.51 delay 3 seconds @10:06:02.52 ATV.dial :bleach @10:06:02.91 AS.seek 0.820ml @10:06:06.22 delay 3 seconds @10:06:09.24 ATV.dial PCR @10:06:09.56 AS.reconfigure ASconfig @10:06:09.59 AS.seek empty @10:06:57.69 PCR.program :bleachProfile @10:06:57.75 PCR program should complete in < 2:45 @10:06:57.76 PCR.run 10:00 @10:09:58.97 PCR.off @10:09:59.00 AFV.select :carrierSolution @10:09:59.09 delay 2 seconds @10:10:01.10 AS.seek 1.000ml @10:10:59.76 delay 1 second @10:11:00.77 AFV.select :holdingCoil @10:11:00.86 delay 1 second @10:11:01.87 AS.reconfigure ASfast @10:11:01.90 AS.seek 0.000ml @10:11:29.64 delay 3 seconds @10:11:32.65 AFV.select :carrierSolution @10:11:32.74 delay 2 seconds @10:11:34.75 AS.reconfigure ASconfig @10:11:34.78 AS.seek 1.000ml @10:12:33.39 delay 1 second @10:12:34.40 AFV.select :holdingCoil @10:12:34.49 delay 1 second @10:12:35.50 AS.reconfigure ASfast @10:12:35.53 AS.seek 0.000ml @10:13:03.26 delay 3 seconds @10:13:06.27 AFV.select :carrierSolution @10:13:06.36 delay 2 seconds @10:13:08.37 AS.reconfigure ASconfig @10:13:08.40 AS.seek 1.000ml @10:14:07.01 delay 1 second @10:14:08.02 AFV.select :holdingCoil @10:14:08.11 delay 1 second @10:14:09.12 AS.reconfigure ASfast @10:14:09.15 AS.seek 0.000ml @10:14:36.88 delay 3 seconds @10:14:39.89 AFV.select :carrierSolution @10:14:39.98 delay 2 seconds @10:14:41.99 AS.reconfigure ASconfig @10:14:42.02 AS.seek 1.000ml @10:15:40.63 delay 1 second @10:15:41.64 AFV.select :holdingCoil @10:15:41.79 delay 1 second @10:15:42.80 AS.reconfigure ASfast @10:15:42.83 AS.seek 0.000ml @10:16:10.57 delay 3 seconds @10:16:13.58 AFV.select :carrierSolution @10:16:13.67 delay 2 seconds @10:16:16.21 AS.reconfigure ASconfig @10:16:16.24 AS.seek 1.000ml @10:17:14.83 delay 1 second @10:17:15.84 AFV.select :holdingCoil @10:17:15.93 delay 1 second @10:17:16.94 AS.reconfigure ASnorm @10:17:16.98 AS.seek 0.000ml @10:18:08.44 Setting up PCR for ENTem -- writing /var/log/esp/ENTem14mar1510h.pcr @10:18:08.45 Building PCR Slug from ASV with MM2 mix, PP3 primer @10:18:08.46 Priming with carrier @10:18:08.47 ATV.dial ARV @10:18:08.98 ARV.dial :carrier @10:18:09.27 AS.seek 0.500ml @10:18:35.16 AFV.select :carrierSolution @10:18:35.27 delay 2 seconds @10:18:37.28 AS.reconfigure ASconfig @10:18:37.32 AS.seek 1.000ml @10:19:06.73 delay 1 second @10:19:07.74 AFV.select :holdingCoil @10:19:07.84 delay 1 second @10:19:08.85 ATV.dial :waste @10:19:10.12 AS.reconfigure ASnorm @10:19:10.15 AS.seek empty @10:20:01.62 AFV.select :carrierSolution @10:20:01.73 delay 2 seconds @10:20:03.74 AS.reconfigure ASconfig @10:20:03.77 AS.seek 0.500ml @10:20:33.28 delay 1 second @10:20:34.29 AFV.select :holdingCoil @10:20:34.39 Building Slug @10:20:34.40 ATV.dial :ARV @10:20:35.75 delay 2 seconds @10:20:37.76 ARV.dial :air1 @10:20:38.09 delay 2 seconds @10:20:40.15 AS.reconfigure ASslow2 @10:20:40.18 AS.seek 0.506ml @10:20:46.57 delay 2 seconds @10:20:48.58 ARV.dial :MM2 @10:20:49.89 delay 2 seconds @10:20:51.90 Adding MM2 @10:20:51.96 AS.seek 0.524ml @10:21:10.74 delay 25 seconds @10:21:35.75 ARV.dial :PP3 @10:21:36.08 Adding PP3 @10:21:36.09 delay 2 seconds @10:21:38.14 AS.seek 0.530ml @10:21:44.38 delay 10 seconds @10:21:54.39 ARV.dial :air2 @10:21:54.81 adding ARV air @10:21:54.82 delay 2 seconds @10:21:56.88 AS.seek 0.536ml @10:22:03.16 delay 5 seconds @10:22:08.17 ARV.dial :carrier @10:22:09.21 delay 7 seconds @10:22:16.27 AS.seek 0.594ml @10:23:15.56 delay 5 seconds @10:23:20.57 ATV.dial :ASV @10:23:20.89 delay 2 seconds @10:23:22.95 AS.seek 0.600ml @10:23:29.23 delay 4 seconds @10:23:33.24 ATV.dial ARV @10:23:33.56 delay 2 seconds @10:23:35.57 ARV.dial :carrier @10:23:35.58 delay 2 seconds @10:23:37.63 AS.seek 0.650ml @10:24:28.80 delay 4 seconds @10:24:32.81 ATV.dial PCR @10:24:33.42 ASV.dial :waste1 @10:24:33.43 ARV.dial :air1 @10:24:33.87 Positioning Slug by 0.324ml @10:24:33.93 AS.seek 0.326ml @10:30:04.41 delay 5 seconds @10:30:09.42 ATV.dial ARV @10:30:09.93 ASV.dial :PCRlock @10:30:10.43 delay 5 seconds @10:30:15.44 Running ENTem ProfileEM60C thermocycling @10:30:15.45 PCR.program ProfileEM60C @10:30:15.56 ProfileEM60C should complete in < 2:11:38 @10:30:15.57 PCR.run 2:49:32.5 @12:25:55.07 PCR.off @12:25:55.61 PCR for ENTem completed -- closed /var/log/esp/ENTem14mar1510h.pcr @12:25:55.66 Cleaning PCR AFV.select :carrierSolution @12:25:55.75 delay 2 seconds @12:25:57.76 AS.reconfigure ASconfig @12:25:57.79 AS.seek 0.500ml @12:26:08.22 delay 1 second @12:26:09.23 AFV.select :holdingCoil @12:26:09.32 ASV.dial :waste1 @12:26:09.81 ATV.dial :air3 @12:26:10.75 AS.reconfigure ASslow1 @12:26:10.78 AS.seek 0.510ml @12:26:13.08 delay 2 seconds @12:26:15.09 ATV.dial :bleach @12:26:15.49 AS.seek 0.810ml @12:27:16.90 delay 3 seconds @12:27:19.91 ATV.dial :air3 @12:27:20.25 delay 3 seconds @12:27:23.31 AS.seek 0.835ml @12:27:28.64 delay 3 seconds @12:27:31.65 ATV.dial :bleach @12:27:32.05 AS.seek 0.820ml @12:27:35.36 delay 3 seconds @12:27:38.37 ARV.dial :waste @12:27:38.87 delay 3 seconds @12:27:41.88 ATV.dial :ARV @12:27:42.60 AS.reconfigure ASconfig @12:27:42.64 AS.seek 0.470ml @12:28:03.31 AFV.select :carrierSolution @12:28:03.40 delay 2 seconds @12:28:05.41 AS.seek 1.000ml @12:28:36.62 delay 1 second @12:28:37.63 AFV.select :holdingCoil @12:28:37.72 delay 1 second @12:28:38.73 AS.reconfigure ASfast @12:28:38.76 AS.seek 0.000ml @12:29:06.49 AFV.select :carrierSolution @12:29:06.59 delay 2 seconds @12:29:08.60 AS.reconfigure ASconfig @12:29:08.63 AS.seek 1.000ml @12:30:07.24 delay 1 second @12:30:08.25 AFV.select :holdingCoil @12:30:08.34 delay 1 second @12:30:09.35 AS.reconfigure ASfast @12:30:09.38 AS.seek 0.000ml @12:30:37.12 AFV.select :carrierSolution @12:30:37.22 delay 2 seconds @12:30:39.23 AS.reconfigure ASconfig @12:30:39.26 AS.seek 1.000ml @12:31:37.86 delay 1 second @12:31:38.87 AFV.select :holdingCoil @12:31:38.96 delay 1 second @12:31:39.97 AS.reconfigure ASfast @12:31:40.00 AS.seek 0.000ml @12:32:07.74 ARV.dial between carrier and air1 @12:32:08.25 ATV.dial :air3 @12:32:09.19 AS.reconfigure ASslow1 @12:32:09.22 AS.seek 0.010ml @12:32:11.55 delay 2 seconds @12:32:13.56 ATV.dial :bleach @12:32:13.96 AS.seek 0.810ml @12:34:57.34 delay 3 seconds @12:35:00.35 ATV.dial :air3 @12:35:00.69 delay 3 seconds @12:35:03.75 AS.seek 0.835ml @12:35:09.06 delay 3 seconds @12:35:12.07 ATV.dial :bleach @12:35:12.47 AS.seek 0.820ml @12:35:15.79 delay 3 seconds @12:35:18.80 ATV.dial PCR @12:35:19.12 AS.reconfigure ASconfig @12:35:19.15 AS.seek empty @12:36:07.28 PCR.program :bleachProfile @12:36:07.33 PCR program should complete in < 2:45 @12:36:07.34 PCR.run 10:00 @12:39:06.11 PCR.off @12:39:06.14 AFV.select :carrierSolution @12:39:06.23 delay 2 seconds @12:39:08.24 AS.seek 1.000ml @12:40:06.90 delay 1 second @12:40:07.91 AFV.select :holdingCoil @12:40:08.00 delay 1 second @12:40:09.01 AS.reconfigure ASfast @12:40:09.05 AS.seek 0.000ml @12:40:36.81 delay 3 seconds @12:40:39.82 AFV.select :carrierSolution @12:40:39.91 delay 2 seconds @12:40:41.92 AS.reconfigure ASconfig @12:40:41.95 AS.seek 1.000ml @12:41:40.56 delay 1 second @12:41:41.57 AFV.select :holdingCoil @12:41:41.66 delay 1 second @12:41:42.67 AS.reconfigure ASfast @12:41:42.70 AS.seek 0.000ml @12:42:10.42 delay 3 seconds @12:42:13.43 AFV.select :carrierSolution @12:42:13.52 delay 2 seconds @12:42:15.53 AS.reconfigure ASconfig @12:42:15.57 AS.seek 1.000ml @12:43:14.18 delay 1 second @12:43:15.19 AFV.select :holdingCoil @12:43:15.28 delay 1 second @12:43:16.29 AS.reconfigure ASfast @12:43:16.32 AS.seek 0.000ml @12:43:44.05 delay 3 seconds @12:43:47.06 AFV.select :carrierSolution @12:43:47.15 delay 2 seconds @12:43:49.16 AS.reconfigure ASconfig @12:43:49.19 AS.seek 1.000ml @12:44:47.77 delay 1 second @12:44:48.78 AFV.select :holdingCoil @12:44:48.87 delay 1 second @12:44:49.88 AS.reconfigure ASfast @12:44:49.91 AS.seek 0.000ml @12:45:17.64 delay 3 seconds @12:45:20.65 AFV.select :carrierSolution @12:45:20.74 delay 2 seconds @12:45:22.75 AS.reconfigure ASconfig @12:45:22.78 AS.seek 1.000ml @12:46:21.38 delay 1 second @12:46:22.39 AFV.select :holdingCoil @12:46:22.48 delay 1 second @12:46:23.49 AS.reconfigure ASnorm @12:46:23.52 AS.seek 0.000ml @12:47:14.98 Setting up PCR for IPCap -- writing /var/log/esp/IPCap14mar1512h.pcr @12:47:14.99 Building PCR Slug from ASV with MM1 mix, PP2 primer @12:47:15.00 Priming with carrier @12:47:15.01 ATV.dial ARV @12:47:15.61 ARV.dial :carrier @12:47:15.88 AS.seek 0.500ml @12:47:41.76 AFV.select :carrierSolution @12:47:41.87 delay 2 seconds @12:47:43.88 AS.reconfigure ASconfig @12:47:43.92 AS.seek 1.000ml @12:48:13.36 delay 1 second @12:48:14.37 AFV.select :holdingCoil @12:48:14.47 delay 1 second @12:48:15.48 ATV.dial :waste @12:48:16.74 AS.reconfigure ASnorm @12:48:16.77 AS.seek empty @12:49:08.26 AFV.select :carrierSolution @12:49:08.36 delay 2 seconds @12:49:10.37 AS.reconfigure ASconfig @12:49:10.40 AS.seek 0.500ml @12:49:39.88 delay 1 second @12:49:40.89 AFV.select :holdingCoil @12:49:40.99 Building Slug @12:49:41.00 ATV.dial :ARV @12:49:42.24 delay 2 seconds @12:49:44.25 ARV.dial :air1 @12:49:44.57 delay 2 seconds @12:49:46.63 AS.reconfigure ASslow2 @12:49:46.66 AS.seek 0.506ml @12:49:53.31 delay 2 seconds @12:49:55.32 ARV.dial :MM1 @12:49:55.92 delay 2 seconds @12:49:57.93 Adding MM1 @12:49:57.99 AS.seek 0.524ml @12:50:16.66 delay 25 seconds @12:50:41.67 ARV.dial :PP2 @12:50:41.99 Adding PP2 @12:50:42.00 delay 2 seconds @12:50:44.06 AS.seek 0.530ml @12:50:50.51 delay 10 seconds @12:51:00.52 ARV.dial :air2 @12:51:00.93 adding ARV air @12:51:00.94 delay 2 seconds @12:51:03.00 AS.seek 0.536ml @12:51:09.26 delay 5 seconds @12:51:14.27 ARV.dial :carrier @12:51:15.30 delay 7 seconds @12:51:22.36 AS.seek 0.594ml @12:52:21.71 delay 5 seconds @12:52:26.72 ATV.dial :ASV @12:52:27.05 delay 2 seconds @12:52:29.11 AS.seek 0.600ml @12:52:35.36 delay 4 seconds @12:52:39.37 ATV.dial ARV @12:52:39.69 delay 2 seconds @12:52:41.70 ARV.dial :carrier @12:52:41.71 delay 2 seconds @12:52:43.76 AS.seek 0.650ml @12:53:34.97 delay 4 seconds @12:53:38.98 ATV.dial PCR @12:53:39.49 ASV.dial :waste1 @12:53:39.50 ARV.dial :air1 @12:53:39.84 Positioning Slug by 0.324ml @12:53:39.89 AS.seek 0.326ml @12:59:10.38 delay 5 seconds @12:59:15.39 ATV.dial ARV @12:59:15.91 ASV.dial :PCRlock @12:59:16.41 delay 5 seconds @12:59:21.42 Running IPCap Profile59C thermocycling @12:59:21.44 PCR.program Profile59C @12:59:21.53 Profile59C should complete in < 1:39:08 @12:59:21.54 PCR.run 2:08:55 @14:23:18.56 PCR.off @14:23:19.09 PCR for IPCap completed -- closed /var/log/esp/IPCap14mar1512h.pcr @14:23:19.15 Cleaning PCR AFV.select :carrierSolution @14:23:19.24 delay 2 seconds @14:23:21.25 AS.reconfigure ASconfig @14:23:21.28 AS.seek 0.500ml @14:23:31.71 delay 1 second @14:23:32.72 AFV.select :holdingCoil @14:23:32.81 ASV.dial :waste1 @14:23:33.29 ATV.dial :air3 @14:23:34.23 AS.reconfigure ASslow1 @14:23:34.26 AS.seek 0.510ml @14:23:36.56 delay 2 seconds @14:23:38.56 ATV.dial :bleach @14:23:38.93 AS.seek 0.810ml @14:24:40.33 delay 3 seconds @14:24:43.34 ATV.dial :air3 @14:24:43.68 delay 3 seconds @14:24:46.74 AS.seek 0.835ml @14:24:52.07 delay 3 seconds @14:24:55.08 ATV.dial :bleach @14:24:55.48 AS.seek 0.820ml @14:24:58.79 delay 3 seconds @14:25:01.80 ARV.dial :waste @14:25:02.40 delay 3 seconds @14:25:05.41 ATV.dial :ARV @14:25:06.14 AS.reconfigure ASconfig @14:25:06.18 AS.seek 0.470ml @14:25:26.86 AFV.select :carrierSolution @14:25:26.95 delay 2 seconds @14:25:28.96 AS.seek 1.000ml @14:26:00.17 delay 1 second @14:26:01.18 AFV.select :holdingCoil @14:26:01.27 delay 1 second @14:26:02.28 AS.reconfigure ASfast @14:26:02.31 AS.seek 0.000ml @14:26:30.05 AFV.select :carrierSolution @14:26:30.14 delay 2 seconds @14:26:32.15 AS.reconfigure ASconfig @14:26:32.18 AS.seek 1.000ml @14:27:30.78 delay 1 second @14:27:32.36 AFV.select :holdingCoil @14:27:32.45 delay 1 second @14:27:33.46 AS.reconfigure ASfast @14:27:33.49 AS.seek 0.000ml @14:28:01.20 AFV.select :carrierSolution @14:28:01.30 delay 2 seconds @14:28:03.31 AS.reconfigure ASconfig @14:28:03.34 AS.seek 1.000ml @14:29:01.93 delay 1 second @14:29:02.94 AFV.select :holdingCoil @14:29:03.03 delay 1 second @14:29:04.04 AS.reconfigure ASfast @14:29:04.08 AS.seek 0.000ml @14:29:31.82 ARV.dial between carrier and air1 @14:29:32.23 ATV.dial :air3 @14:29:33.16 AS.reconfigure ASslow1 @14:29:33.19 AS.seek 0.010ml @14:29:35.53 delay 2 seconds @14:29:37.54 ATV.dial :bleach @14:29:37.92 AS.seek 0.810ml @14:32:21.32 delay 3 seconds @14:32:24.33 ATV.dial :air3 @14:32:24.68 delay 3 seconds @14:32:27.74 AS.seek 0.835ml @14:32:33.08 delay 3 seconds @14:32:36.09 ATV.dial :bleach @14:32:36.48 AS.seek 0.820ml @14:32:39.79 delay 3 seconds @14:32:42.80 ATV.dial PCR @14:32:43.12 AS.reconfigure ASconfig @14:32:43.15 AS.seek empty @14:33:31.28 PCR.program :bleachProfile @14:33:31.33 PCR program should complete in < 2:45 @14:33:31.34 PCR.run 10:00 @14:36:29.53 PCR.off @14:36:29.56 AFV.select :carrierSolution @14:36:29.65 delay 2 seconds @14:36:31.66 AS.seek 1.000ml @14:37:30.31 delay 1 second @14:37:31.32 AFV.select :holdingCoil @14:37:31.41 delay 1 second @14:37:32.42 AS.reconfigure ASfast @14:37:32.45 AS.seek 0.000ml @14:38:00.15 delay 3 seconds @14:38:03.16 AFV.select :carrierSolution @14:38:03.25 delay 2 seconds @14:38:05.26 AS.reconfigure ASconfig @14:38:05.29 AS.seek 1.000ml @14:39:03.88 delay 1 second @14:39:04.89 AFV.select :holdingCoil @14:39:04.98 delay 1 second @14:39:05.99 AS.reconfigure ASfast @14:39:06.02 AS.seek 0.000ml @14:39:33.75 delay 3 seconds @14:39:36.76 AFV.select :carrierSolution @14:39:36.85 delay 2 seconds @14:39:38.86 AS.reconfigure ASconfig @14:39:38.89 AS.seek 1.000ml @14:40:37.50 delay 1 second @14:40:38.51 AFV.select :holdingCoil @14:40:38.60 delay 1 second @14:40:39.61 AS.reconfigure ASfast @14:40:39.64 AS.seek 0.000ml @14:41:07.37 delay 3 seconds @14:41:10.38 AFV.select :carrierSolution @14:41:10.47 delay 2 seconds @14:41:12.48 AS.reconfigure ASconfig @14:41:12.51 AS.seek 1.000ml @14:42:11.09 delay 1 second @14:42:12.10 AFV.select :holdingCoil @14:42:12.19 delay 1 second @14:42:13.20 AS.reconfigure ASfast @14:42:13.23 AS.seek 0.000ml @14:42:40.96 delay 3 seconds @14:42:43.97 AFV.select :carrierSolution @14:42:44.06 delay 2 seconds @14:42:46.07 AS.reconfigure ASconfig @14:42:46.10 AS.seek 1.000ml @14:43:44.71 delay 1 second @14:43:45.72 AFV.select :holdingCoil @14:43:45.81 delay 1 second @14:43:46.82 AS.reconfigure ASnorm @14:43:46.85 AS.seek 0.000ml @14:44:38.32 Setting up PCR for IPCem -- writing /var/log/esp/IPCem14mar1514h.pcr @14:44:38.33 Building PCR Slug from ASV with MM3 mix, PP5 primer @14:44:38.34 Priming with carrier @14:44:38.35 ATV.dial ARV @14:44:38.94 ARV.dial :carrier @14:44:39.23 AS.seek 0.500ml @14:45:05.13 AFV.select :carrierSolution @14:45:05.24 delay 2 seconds @14:45:07.25 AS.reconfigure ASconfig @14:45:07.29 AS.seek 1.000ml @14:45:36.72 delay 1 second @14:45:37.73 AFV.select :holdingCoil @14:45:37.82 delay 1 second @14:45:38.83 ATV.dial :waste @14:45:40.09 AS.reconfigure ASnorm @14:45:40.12 AS.seek empty @14:46:31.61 AFV.select :carrierSolution @14:46:31.71 delay 2 seconds @14:46:33.72 AS.reconfigure ASconfig @14:46:33.75 AS.seek 0.500ml @14:47:03.22 delay 1 second @14:47:04.23 AFV.select :holdingCoil @14:47:04.32 Building Slug @14:47:04.33 ATV.dial :ARV @14:47:05.58 delay 2 seconds @14:47:07.59 ARV.dial :air1 @14:47:07.92 delay 2 seconds @14:47:09.98 AS.reconfigure ASslow2 @14:47:10.01 AS.seek 0.506ml @14:47:16.50 delay 2 seconds @14:47:18.51 ARV.dial :MM3 @14:47:19.36 delay 2 seconds @14:47:21.37 Adding MM3 @14:47:21.43 AS.seek 0.524ml @14:47:40.03 delay 25 seconds @14:48:05.04 ARV.dial :PP5 @14:48:05.45 Adding PP5 @14:48:05.46 delay 2 seconds @14:48:07.52 AS.seek 0.530ml @14:48:13.95 delay 10 seconds @14:48:23.96 ARV.dial :air3 @14:48:24.28 adding ARV air @14:48:24.29 delay 2 seconds @14:48:26.86 AS.seek 0.536ml @14:48:33.09 delay 5 seconds @14:48:38.10 ARV.dial :carrier @14:48:39.12 delay 7 seconds @14:48:46.18 AS.seek 0.594ml @14:49:45.51 delay 5 seconds @14:49:50.52 ATV.dial :ASV @14:49:50.84 delay 2 seconds @14:49:52.90 AS.seek 0.600ml @14:49:59.26 delay 4 seconds @14:50:03.27 ATV.dial ARV @14:50:03.61 delay 2 seconds @14:50:05.62 ARV.dial :carrier @14:50:05.63 delay 2 seconds @14:50:07.68 AS.seek 0.650ml @14:50:58.99 delay 4 seconds @14:51:03.00 ATV.dial PCR @14:51:03.49 ASV.dial :waste1 @14:51:03.50 ARV.dial :air1 @14:51:03.94 Positioning Slug by 0.324ml @14:51:04.00 AS.seek 0.326ml @14:56:34.82 delay 5 seconds @14:56:39.83 ATV.dial ARV @14:56:40.35 ASV.dial :PCRlock @14:56:40.84 delay 5 seconds @14:56:45.85 Running IPCem ProfileEM59C thermocycling @14:56:45.86 PCR.program ProfileEM59C @14:56:45.97 ProfileEM59C should complete in < 2:11:38 @14:56:45.98 PCR.run 2:49:32.5 @16:54:23.52 PCR.off @16:54:24.06 PCR for IPCem completed -- closed /var/log/esp/IPCem14mar1514h.pcr @16:54:24.07 Full MFB Decontamination @16:54:24.08 Decon extraction column and decon AIV @16:54:24.09 SPE.hold 2:46:40,55.0C @16:54:24.12 ASV.dial :SPEdelivery @16:54:24.80 delay 20 seconds @16:54:44.81 AFV.select :carrierSolution @16:54:44.90 delay 2 seconds @16:54:46.91 AS.reconfigure ASconfig @16:54:46.95 AS.seek 0.500ml @16:54:57.36 delay 1 second @16:54:58.37 AFV.select :holdingCoil @16:54:58.46 ATV.dial :air1 @16:54:59.97 AS.reconfigure ASslow1 @16:55:00.00 AS.seek 0.520ml @16:55:04.34 ATV.dial :ASV @16:55:05.61 ASV.dial :SPErecovery @16:55:06.16 AS.seek 0.620ml @16:55:26.79 ATV.dial :waste @16:55:28.25 AS.reconfigure ASnorm @16:55:28.28 AS.seek empty @16:56:00.35 AFV.select :holdingCoil @16:56:00.36 ATV.dial :waste @16:56:00.37 AS.reconfigure ASconfig @16:56:00.40 AS.seek 0.000ml @16:56:00.68 AFV.select :carrierSolution @16:56:00.79 delay 2 seconds @16:56:02.80 AS.seek 0.500ml @16:56:32.19 delay 1 second @16:56:33.20 AFV.select :holdingCoil @16:56:33.29 ATV.dial :air3 @16:56:33.85 AS.reconfigure ASslow1 @16:56:33.88 AS.seek 0.520ml @16:56:38.19 ATV.dial :air3 @16:56:38.25 AS.seek 0.530ml @16:56:40.55 delay 2 seconds @16:56:42.56 ATV.dial :bleach @16:56:42.93 AS.seek 0.710ml @16:57:19.88 delay 3 seconds @16:57:22.89 ATV.dial :air3 @16:57:23.24 delay 3 seconds @16:57:26.30 AS.seek 0.735ml @16:57:31.65 delay 3 seconds @16:57:34.66 ATV.dial :bleach @16:57:35.05 AS.seek 0.720ml @16:57:38.33 delay 3 seconds @16:57:41.34 ATV.dial ASV @16:57:42.30 ASV.dial :SPErecovery @16:57:42.36 AS.seek 0.365ml @16:58:55.00 ATV.dial :waste @16:58:56.47 AS.reconfigure ASnorm @16:58:56.50 AS.seek empty @16:59:15.55 ATV.dial :air3 @16:59:16.11 AS.reconfigure ASslow1 @16:59:16.14 AS.seek 0.009ml @16:59:18.47 delay 2 seconds @16:59:20.48 ATV.dial :bleach @16:59:20.96 AS.seek 0.579ml @17:01:17.48 delay 3 seconds @17:01:20.49 ATV.dial :air3 @17:01:20.81 delay 3 seconds @17:01:23.87 AS.seek 0.604ml @17:01:29.28 delay 3 seconds @17:01:32.29 ATV.dial :bleach @17:01:32.69 AS.seek 0.589ml @17:01:35.98 delay 3 seconds @17:01:38.99 ATV.dial :ASV @17:01:39.86 ASV.dial :wasteR @17:01:40.63 AS.seek 0.000ml @17:03:41.07 delay 1:00 @17:04:41.08 ATV.dial ASV @17:04:41.09 AFV.select :carrierSolution @17:04:41.18 delay 2 seconds @17:04:43.19 AS.reconfigure ASconfig @17:04:43.22 AS.seek 1.000ml @17:05:41.85 delay 1 second @17:05:42.86 AFV.select :holdingCoil @17:05:42.95 delay 1 second @17:05:43.96 ASV.dial :wasteR @17:05:43.97 AS.reconfigure ASslow1 @17:05:44.00 AS.seek 0.000ml @17:09:08.31 delay 10 seconds @17:09:18.32 ATV.dial :air3 @17:09:19.44 AS.seek 0.010ml @17:09:21.81 delay 2 seconds @17:09:23.82 ATV.dial :bleach @17:09:24.20 AS.seek 0.580ml @17:11:20.71 delay 3 seconds @17:11:23.72 ATV.dial :air3 @17:11:24.04 delay 3 seconds @17:11:27.10 AS.seek 0.605ml @17:11:32.46 delay 3 seconds @17:11:35.47 ATV.dial :bleach @17:11:35.95 AS.seek 0.590ml @17:11:39.27 delay 3 seconds @17:11:42.28 ATV.dial ASV @17:11:43.16 ASV.dial :SPEdelivery @17:11:43.64 AS.seek 0.000ml @17:13:44.34 delay 1:00 @17:14:44.35 AFV.select :carrierSolution @17:14:44.44 delay 2 seconds @17:14:46.45 AS.reconfigure ASconfig @17:14:46.48 AS.seek 1.000ml @17:15:45.04 delay 1 second @17:15:46.05 AFV.select :holdingCoil @17:15:46.14 delay 1 second @17:15:47.15 ATV.dial ASV @17:15:47.16 ASV.dial :SPEdelivery @17:15:47.17 AS.reconfigure ASslow1 @17:15:47.20 AS.seek 0.000ml @17:19:11.42 delay 10 seconds @17:19:21.43 ATV.dial :air2 @17:19:22.14 AS.reconfigure ASslow0 @17:19:22.17 AS.seek 0.800ml @17:20:44.11 delay 3 seconds @17:20:47.12 ATV.dial :ASV @17:20:47.84 ASV.dial :SPEdelivery @17:20:47.86 AS.reconfigure ASslow1 @17:20:47.89 AS.seek 0.030ml @17:23:25.17 delay 20 seconds @17:23:45.18 ATV.dial :waste,via => air3 @17:23:46.61 AS.reconfigure ASconfig @17:23:46.64 AS.seek empty @17:23:48.74 SPE.coast @17:23:48.82 Removing Bleach from Mixing Coil @17:23:48.83 AFV.select :carrierSolution @17:23:48.92 delay 2 seconds @17:23:50.93 AS.seek 0.500ml @17:24:20.41 delay 1 second @17:24:21.42 AFV.select :holdingCoil @17:24:21.51 ATV.dial :ASV @17:24:22.94 ASV.dial :SPErecovery @17:24:23.92 AS.reconfigure ASslow1 @17:24:23.95 AS.seek 0.730ml @17:25:11.11 ASV.dial :wasteD @17:25:11.78 AS.reconfigure ASnorm @17:25:11.81 AS.seek 0.000ml @17:25:49.43 AFV.select :carrierSolution @17:25:49.53 delay 2 seconds @17:25:51.54 AS.reconfigure ASconfig @17:25:51.57 AS.seek 0.500ml @17:26:21.00 delay 1 second @17:26:22.01 AFV.select :holdingCoil @17:26:22.10 ATV.dial ASV @17:26:22.11 ASV.dial :SPErecovery @17:26:22.82 AS.reconfigure ASslow0 @17:26:22.85 AS.seek 0.300ml @17:26:43.53 ATV.dial :air2 @17:26:44.29 AS.reconfigure ASslow1 @17:26:44.32 AS.seek 0.320ml @17:26:48.66 ATV.dial ASV @17:26:49.41 AS.reconfigure ASslow0 @17:26:49.45 AS.seek 0.570ml @17:27:15.24 ASV.dial :wasteD @17:27:15.90 AS.reconfigure ASnorm @17:27:15.93 AS.seek 0.000ml @17:27:45.39 AFV.select :carrierSolution @17:27:45.49 delay 2 seconds @17:27:47.50 AS.reconfigure ASconfig @17:27:47.53 AS.seek 0.500ml @17:28:16.97 delay 1 second @17:28:17.98 AFV.select :holdingCoil @17:28:18.07 ATV.dial ASV @17:28:18.08 ASV.dial :SPErecovery @17:28:18.78 AS.reconfigure ASslow0 @17:28:18.81 AS.seek 0.300ml @17:28:39.50 ATV.dial :air2 @17:28:40.25 AS.reconfigure ASslow1 @17:28:40.28 AS.seek 0.320ml @17:28:44.55 ATV.dial ASV @17:28:45.31 AS.reconfigure ASslow0 @17:28:45.34 AS.seek 0.570ml @17:29:11.09 ASV.dial :wasteD @17:29:12.02 AS.reconfigure ASnorm @17:29:12.05 AS.seek 0.000ml @17:29:41.46 ATV.dial :air2 @17:29:42.17 AS.reconfigure ASslow0 @17:29:42.20 AS.seek 0.300ml @17:30:13.11 ATV.dial :ASV @17:30:13.93 ASV.dial :wasteD @17:30:13.95 AS.reconfigure ASconfig @17:30:13.98 AS.seek 0.050ml @17:30:28.86 ATV.dial :waste @17:30:30.30 AS.seek 0.000ml @17:30:33.52 ASV.dial :SPEdelivery @17:30:34.36 deconLysateINT @17:30:34.37 ATV.dial :air3 @17:30:34.95 AS.reconfigure ASslow1 @17:30:34.98 AS.seek 0.010ml @17:30:37.32 delay 2 seconds @17:30:39.33 ATV.dial :bleach @17:30:39.80 AS.seek 0.960ml @17:33:53.80 delay 3 seconds @17:33:56.81 ATV.dial :air3 @17:33:57.28 delay 3 seconds @17:34:00.34 AS.seek 0.985ml @17:34:05.70 delay 3 seconds @17:34:08.71 ATV.dial :bleach @17:34:09.09 AS.seek 0.970ml @17:34:12.42 delay 3 seconds @17:34:15.43 ATV.dial :AIV @17:34:16.51 AIV.dial :coilToMFB @17:34:16.52 AWV.select :waste AFV.select :carrierSolution @17:34:16.61 delay 2 seconds @17:34:18.62 AS.reconfigure ASconfig @17:34:18.65 AS.seek 1.000ml @17:34:20.72 delay 1 second @17:34:21.73 AFV.select :holdingCoil @17:34:21.82 delay 1 second @17:34:22.83 AS.reconfigure ASslow0 @17:34:22.86 AS.seek empty @17:36:05.26 AFV.select :carrierSolution @17:36:05.35 delay 2 seconds @17:36:07.36 AS.reconfigure ASconfig @17:36:07.39 AS.seek 1.000ml @17:37:06.05 delay 1 second @17:37:07.06 AFV.select :holdingCoil @17:37:07.15 delay 1 second @17:37:08.16 AS.reconfigure ASslow0 @17:37:08.19 AS.seek empty @17:38:50.61 AFV.select :carrierSolution @17:38:50.71 delay 2 seconds @17:38:52.72 AS.reconfigure ASconfig @17:38:52.75 AS.seek 1.000ml @17:39:51.38 delay 1 second @17:39:52.39 AFV.select :holdingCoil @17:39:52.48 delay 1 second @17:39:53.49 AS.reconfigure ASslow0 @17:39:53.52 AS.seek empty @17:41:35.92 ATV.dial :air2 @17:41:36.49 AS.reconfigure ASslow1 @17:41:36.52 AS.seek 0.009ml @17:41:38.86 AS.reconfigure ASslow0 @17:41:38.90 AS.seek 1.000ml @17:43:20.20 ATV.dial AIV @17:43:20.83 AS.seek 0.020ml @17:45:01.63 ATV.dial :waste @17:45:02.89 AS.seek 0.000ml @17:45:05.23 AWV.select :air @17:45:05.38 Cleaning PCR AFV.select :carrierSolution @17:45:05.47 delay 2 seconds @17:45:07.48 AS.reconfigure ASconfig @17:45:07.52 AS.seek 0.500ml @17:45:36.96 delay 1 second @17:45:37.97 AFV.select :holdingCoil @17:45:38.06 ASV.dial :waste1 @17:45:38.40 ATV.dial :air3 @17:45:38.98 AS.reconfigure ASslow1 @17:45:39.01 AS.seek 0.510ml @17:45:41.32 delay 2 seconds @17:45:43.33 ATV.dial :bleach @17:45:43.81 AS.seek 0.810ml @17:46:45.29 delay 3 seconds @17:46:48.30 ATV.dial :air3 @17:46:48.62 delay 3 seconds @17:46:51.68 AS.seek 0.835ml @17:46:56.98 delay 3 seconds @17:46:59.99 ATV.dial :bleach @17:47:00.38 AS.seek 0.820ml @17:47:03.70 delay 3 seconds @17:47:06.71 ARV.dial :waste @17:47:07.20 delay 3 seconds @17:47:10.21 ATV.dial :ARV @17:47:11.06 AS.reconfigure ASconfig @17:47:11.09 AS.seek 0.470ml @17:47:31.78 AFV.select :carrierSolution @17:47:31.87 delay 2 seconds @17:47:33.88 AS.seek 1.000ml @17:48:05.04 delay 1 second @17:48:06.05 AFV.select :holdingCoil @17:48:06.14 delay 1 second @17:48:07.15 AS.reconfigure ASfast @17:48:07.18 AS.seek 0.000ml @17:48:34.92 AFV.select :carrierSolution @17:48:35.02 delay 2 seconds @17:48:37.03 AS.reconfigure ASconfig @17:48:37.06 AS.seek 1.000ml @17:49:35.65 delay 1 second @17:49:36.66 AFV.select :holdingCoil @17:49:36.75 delay 1 second @17:49:37.76 AS.reconfigure ASfast @17:49:37.79 AS.seek 0.000ml @17:50:05.52 AFV.select :carrierSolution @17:50:05.62 delay 2 seconds @17:50:07.63 AS.reconfigure ASconfig @17:50:07.66 AS.seek 1.000ml @17:51:06.25 delay 1 second @17:51:07.26 AFV.select :holdingCoil @17:51:07.35 delay 1 second @17:51:08.36 AS.reconfigure ASfast @17:51:08.39 AS.seek 0.000ml @17:51:36.13 ARV.dial between carrier and air1 @17:51:36.54 ATV.dial :air3 @17:51:37.46 AS.reconfigure ASslow1 @17:51:37.49 AS.seek 0.010ml @17:51:39.83 delay 2 seconds @17:51:41.84 ATV.dial :bleach @17:51:42.32 AS.seek 0.810ml @17:54:25.72 delay 3 seconds @17:54:28.73 ATV.dial :air3 @17:54:29.06 delay 3 seconds @17:54:32.12 AS.seek 0.835ml @17:54:37.47 delay 3 seconds @17:54:40.48 ATV.dial :bleach @17:54:40.97 AS.seek 0.820ml @17:54:44.26 delay 3 seconds @17:54:47.27 ATV.dial PCR @17:54:47.60 AS.reconfigure ASconfig @17:54:47.63 AS.seek empty @17:55:35.76 PCR.program :bleachProfile @17:55:35.81 PCR program should complete in < 2:45 @17:55:35.82 PCR.run 10:00 @17:58:37.03 PCR.off @17:58:37.06 AFV.select :carrierSolution @17:58:37.15 delay 2 seconds @17:58:39.16 AS.seek 1.000ml @17:59:37.80 delay 1 second @17:59:38.81 AFV.select :holdingCoil @17:59:38.90 delay 1 second @17:59:39.91 AS.reconfigure ASfast @17:59:39.94 AS.seek 0.000ml @18:00:07.66 delay 3 seconds @18:00:10.67 AFV.select :carrierSolution @18:00:10.76 delay 2 seconds @18:00:12.77 AS.reconfigure ASconfig @18:00:12.80 AS.seek 1.000ml @18:01:11.39 delay 1 second @18:01:12.40 AFV.select :holdingCoil @18:01:12.49 delay 1 second @18:01:13.50 AS.reconfigure ASfast @18:01:13.53 AS.seek 0.000ml @18:01:41.26 delay 3 seconds @18:01:44.27 AFV.select :carrierSolution @18:01:44.36 delay 2 seconds @18:01:46.37 AS.reconfigure ASconfig @18:01:46.40 AS.seek 1.000ml @18:02:45.03 delay 1 second @18:02:46.04 AFV.select :holdingCoil @18:02:46.13 delay 1 second @18:02:47.14 AS.reconfigure ASfast @18:02:47.17 AS.seek 0.000ml @18:03:14.90 delay 3 seconds @18:03:17.91 AFV.select :carrierSolution @18:03:18.00 delay 2 seconds @18:03:20.01 AS.reconfigure ASconfig @18:03:20.04 AS.seek 1.000ml @18:04:18.66 delay 1 second @18:04:19.67 AFV.select :holdingCoil @18:04:19.76 delay 1 second @18:04:20.77 AS.reconfigure ASfast @18:04:20.80 AS.seek 0.000ml @18:04:48.53 delay 3 seconds @18:04:51.54 AFV.select :carrierSolution @18:04:51.63 delay 2 seconds @18:04:53.64 AS.reconfigure ASconfig @18:04:53.67 AS.seek 1.000ml @18:05:52.27 delay 1 second @18:05:53.28 AFV.select :holdingCoil @18:05:53.37 delay 1 second @18:05:54.38 AS.reconfigure ASnorm @18:05:54.41 AS.seek 0.000ml @18:06:46.38 AFV.select :carrierSolution @18:06:46.47 delay 2 seconds @18:06:48.48 AS.reconfigure ASconfig @18:06:48.51 AS.seek 1.000ml @18:07:47.12 delay 1 second @18:07:48.13 AFV.select :holdingCoil @18:07:48.22 delay 1 second @18:07:49.23 ATV.dial :waste @18:07:50.13 AS.reconfigure ASnorm @18:07:50.16 AS.seek 0.500ml @18:08:16.09 ATV.dial :waste2 @18:08:17.18 AS.seek 0.000ml @18:08:43.07 Setting up PCR for HF183em -- writing /var/log/esp/HF183em14mar1518h.pcr @18:08:43.08 Building PCR Slug from elutionWater with MM2 mix, PP4 primer @18:08:43.09 Priming with carrier @18:08:43.10 ATV.dial ARV @18:08:43.79 ARV.dial :carrier @18:08:44.08 AS.seek 0.500ml @18:09:10.01 AFV.select :carrierSolution @18:09:10.12 delay 2 seconds @18:09:12.13 AS.reconfigure ASconfig @18:09:12.17 AS.seek 1.000ml @18:09:41.59 delay 1 second @18:09:42.60 AFV.select :holdingCoil @18:09:42.70 delay 1 second @18:09:43.71 ATV.dial :waste @18:09:45.05 AS.reconfigure ASnorm @18:09:45.08 AS.seek empty @18:10:36.55 AFV.select :carrierSolution @18:10:36.65 delay 2 seconds @18:10:38.66 AS.reconfigure ASconfig @18:10:38.69 AS.seek 0.500ml @18:11:08.18 delay 1 second @18:11:09.19 AFV.select :holdingCoil @18:11:09.28 Building Slug @18:11:09.35 ATV.dial :ARV @18:11:10.68 delay 2 seconds @18:11:12.69 ARV.dial :air1 @18:11:13.02 delay 2 seconds @18:11:15.08 AS.reconfigure ASslow2 @18:11:15.11 AS.seek 0.506ml @18:11:21.46 delay 2 seconds @18:11:23.47 ARV.dial :MM2 @18:11:24.69 delay 2 seconds @18:11:26.70 Adding MM2 @18:11:26.76 AS.seek 0.524ml @18:11:45.38 delay 25 seconds @18:12:10.39 ARV.dial :PP4 @18:12:10.72 Adding PP4 @18:12:10.74 delay 2 seconds @18:12:12.79 AS.seek 0.530ml @18:12:19.11 delay 10 seconds @18:12:29.12 ARV.dial :air3 @18:12:29.42 adding ARV air @18:12:29.43 delay 2 seconds @18:12:31.49 AS.seek 0.536ml @18:12:38.17 delay 5 seconds @18:12:43.18 ARV.dial :carrier @18:12:44.21 delay 7 seconds @18:12:51.27 AS.seek 0.594ml @18:13:50.70 delay 5 seconds @18:13:55.71 ATV.dial :elutionWater @18:13:56.77 delay 2 seconds @18:13:58.83 AS.seek 0.600ml @18:14:05.16 delay 4 seconds @18:14:09.17 ATV.dial ARV @18:14:10.23 delay 2 seconds @18:14:12.24 ARV.dial :carrier @18:14:12.25 delay 2 seconds @18:14:14.30 AS.seek 0.650ml @18:15:05.48 delay 4 seconds @18:15:09.49 ATV.dial PCR @18:15:10.00 ASV.dial :waste1 @18:15:10.01 ARV.dial :air1 @18:15:10.45 Positioning Slug by 0.324ml @18:15:10.50 AS.seek 0.326ml @18:20:40.98 delay 5 seconds @18:20:45.99 ATV.dial ARV @18:20:46.51 ASV.dial :PCRlock @18:20:46.93 delay 5 seconds @18:20:51.94 Running HF183em ProfileEM60C2 thermocycling @18:20:51.95 PCR.program ProfileEM60C2 @18:20:52.06 ProfileEM60C2 should complete in < 2:11:38 @18:20:52.07 PCR.run 2:49:32.5 @20:16:44.28 PCR.off @20:16:44.81 PCR for HF183em completed -- closed /var/log/esp/HF183em14mar1518h.pcr @20:16:44.86 Cleaning PCR @20:16:44.87 AFV.select :carrierSolution @20:16:44.96 delay 2 seconds @20:16:46.97 AS.reconfigure ASconfig @20:16:47.00 AS.seek 0.500ml @20:16:57.41 delay 1 second @20:16:58.42 AFV.select :holdingCoil @20:16:58.51 ASV.dial :waste1 @20:16:58.98 ATV.dial :air3 @20:16:59.92 AS.reconfigure ASslow1 @20:16:59.95 AS.seek 0.510ml @20:17:02.25 delay 2 seconds @20:17:04.26 ATV.dial :bleach @20:17:04.66 AS.seek 0.810ml @20:18:06.11 delay 3 seconds @20:18:09.12 ATV.dial :air3 @20:18:09.47 delay 3 seconds @20:18:12.53 AS.seek 0.835ml @20:18:17.92 delay 3 seconds @20:18:20.93 ATV.dial :bleach @20:18:21.33 AS.seek 0.820ml @20:18:24.65 delay 3 seconds @20:18:27.66 ARV.dial :waste @20:18:28.14 delay 3 seconds @20:18:31.15 ATV.dial :ARV @20:18:31.89 AS.reconfigure ASconfig @20:18:31.92 AS.seek 0.470ml @20:18:52.61 AFV.select :carrierSolution @20:18:52.71 delay 2 seconds @20:18:54.72 AS.seek 1.000ml @20:19:25.95 delay 1 second @20:19:26.96 AFV.select :holdingCoil @20:19:27.05 delay 1 second @20:19:28.06 AS.reconfigure ASfast @20:19:28.09 AS.seek 0.000ml @20:19:55.82 AFV.select :carrierSolution @20:19:55.92 delay 2 seconds @20:19:57.94 AS.reconfigure ASconfig @20:19:57.97 AS.seek 1.000ml @20:20:56.56 delay 1 second @20:20:57.57 AFV.select :holdingCoil @20:20:57.66 delay 1 second @20:20:58.67 AS.reconfigure ASfast @20:20:58.70 AS.seek 0.000ml @20:21:26.44 AFV.select :carrierSolution @20:21:26.54 delay 2 seconds @20:21:28.55 AS.reconfigure ASconfig @20:21:28.58 AS.seek 1.000ml @20:22:27.17 delay 1 second @20:22:28.18 AFV.select :holdingCoil @20:22:28.27 delay 1 second @20:22:29.28 AS.reconfigure ASfast @20:22:29.31 AS.seek 0.000ml @20:22:57.03 ARV.dial between carrier and air1 @20:22:57.44 ATV.dial :air3 @20:22:58.39 AS.reconfigure ASslow1 @20:22:58.42 AS.seek 0.010ml @20:23:00.76 delay 2 seconds @20:23:02.77 ATV.dial :bleach @20:23:03.16 AS.seek 0.810ml @20:25:46.54 delay 3 seconds @20:25:49.55 ATV.dial :air3 @20:25:49.90 delay 3 seconds @20:25:52.96 AS.seek 0.835ml @20:25:58.29 delay 3 seconds @20:26:01.30 ATV.dial :bleach @20:26:01.70 AS.seek 0.820ml @20:26:05.01 delay 3 seconds @20:26:08.02 ATV.dial PCR @20:26:08.35 AS.reconfigure ASconfig @20:26:08.96 AS.seek empty @20:26:57.08 PCR.program :bleachProfile @20:26:57.14 PCR program should complete in < 2:45 @20:26:57.15 PCR.run 10:00 @20:29:55.34 PCR.off @20:29:55.37 AFV.select :carrierSolution @20:29:55.46 delay 2 seconds @20:29:57.47 AS.seek 1.000ml @20:30:56.11 delay 1 second @20:30:57.12 AFV.select :holdingCoil @20:30:57.21 delay 1 second @20:30:58.22 AS.reconfigure ASfast @20:30:58.25 AS.seek 0.000ml @20:31:25.98 delay 3 seconds @20:31:28.99 AFV.select :carrierSolution @20:31:29.08 delay 2 seconds @20:31:31.09 AS.reconfigure ASconfig @20:31:31.12 AS.seek 1.000ml @20:32:29.71 delay 1 second @20:32:30.72 AFV.select :holdingCoil @20:32:30.81 delay 1 second @20:32:31.82 AS.reconfigure ASfast @20:32:31.86 AS.seek 0.000ml @20:32:59.56 delay 3 seconds @20:33:02.57 AFV.select :carrierSolution @20:33:02.66 delay 2 seconds @20:33:04.67 AS.reconfigure ASconfig @20:33:04.70 AS.seek 1.000ml @20:34:03.30 delay 1 second @20:34:04.31 AFV.select :holdingCoil @20:34:04.40 delay 1 second @20:34:05.41 AS.reconfigure ASfast @20:34:05.45 AS.seek 0.000ml @20:34:33.19 delay 3 seconds @20:34:36.20 AFV.select :carrierSolution @20:34:36.29 delay 2 seconds @20:34:38.30 AS.reconfigure ASconfig @20:34:38.33 AS.seek 1.000ml @20:35:36.93 delay 1 second @20:35:37.94 AFV.select :holdingCoil @20:35:38.03 delay 1 second @20:35:39.04 AS.reconfigure ASfast @20:35:39.07 AS.seek 0.000ml @20:36:06.80 delay 3 seconds @20:36:09.81 AFV.select :carrierSolution @20:36:09.90 delay 2 seconds @20:36:11.91 AS.reconfigure ASconfig @20:36:11.95 AS.seek 1.000ml @20:37:10.56 delay 1 second @20:37:11.57 AFV.select :holdingCoil @20:37:11.66 delay 1 second @20:37:12.67 AS.reconfigure ASnorm @20:37:12.70 AS.seek 0.000ml @20:38:04.13 Setting up PCR for ENTap -- writing /var/log/esp/ENTap14mar1520h.pcr @20:38:04.14 Building PCR Slug from elutionWater with MM1 mix, PP1 primer @20:38:04.15 Priming with carrier @20:38:04.16 ATV.dial ARV @20:38:04.68 ARV.dial :carrier @20:38:04.97 AS.seek 0.500ml @20:38:30.85 AFV.select :carrierSolution @20:38:30.96 delay 2 seconds @20:38:32.97 AS.reconfigure ASconfig @20:38:33.01 AS.seek 1.000ml @20:39:02.41 delay 1 second @20:39:03.42 AFV.select :holdingCoil @20:39:03.51 delay 1 second @20:39:04.52 ATV.dial :waste @20:39:05.78 AS.reconfigure ASnorm @20:39:05.81 AS.seek empty @20:39:57.29 AFV.select :carrierSolution @20:39:57.39 delay 2 seconds @20:39:59.40 AS.reconfigure ASconfig @20:39:59.43 AS.seek 0.500ml @20:40:28.91 delay 1 second @20:40:29.92 AFV.select :holdingCoil @20:40:30.01 Building Slug @20:40:30.02 ATV.dial :ARV @20:40:31.27 delay 2 seconds @20:40:33.28 ARV.dial :air1 @20:40:33.61 delay 2 seconds @20:40:35.67 AS.reconfigure ASslow2 @20:40:35.70 AS.seek 0.506ml @20:40:41.97 delay 2 seconds @20:40:43.98 ARV.dial :MM1 @20:40:44.47 delay 2 seconds @20:40:46.48 Adding MM1 @20:40:46.54 AS.seek 0.524ml @20:41:05.11 delay 25 seconds @20:41:30.12 ARV.dial :PP1 @20:41:30.44 Adding PP1 @20:41:30.45 delay 2 seconds @20:41:32.51 AS.seek 0.530ml @20:41:38.74 delay 10 seconds @20:41:48.75 ARV.dial :air1 @20:41:49.07 adding ARV air @20:41:49.08 delay 2 seconds @20:41:51.14 AS.seek 0.536ml @20:41:57.41 delay 5 seconds @20:42:02.42 ARV.dial :carrier @20:42:02.74 delay 7 seconds @20:42:09.80 AS.seek 0.594ml @20:43:09.14 delay 5 seconds @20:43:14.15 ATV.dial :elutionWater @20:43:15.22 delay 2 seconds @20:43:17.28 AS.seek 0.600ml @20:43:23.72 delay 4 seconds @20:43:27.73 ATV.dial ARV @20:43:28.80 delay 2 seconds @20:43:30.81 ARV.dial :carrier @20:43:30.82 delay 2 seconds @20:43:32.87 AS.seek 0.650ml @20:44:24.14 delay 4 seconds @20:44:28.15 ATV.dial PCR @20:44:28.67 ASV.dial :waste1 @20:44:28.68 ARV.dial :air1 @20:44:29.02 Positioning Slug by 0.324ml @20:44:29.07 AS.seek 0.326ml @20:49:59.56 delay 5 seconds @20:50:04.57 ATV.dial ARV @20:50:05.08 ASV.dial :PCRlock @20:50:05.56 delay 5 seconds @20:50:10.57 Running ENTap Profile60C thermocycling @20:50:11.10 PCR.program Profile60C @20:50:11.20 Profile60C should complete in < 1:39:08 @20:50:11.21 PCR.run 2:08:55 @22:12:59.29 PCR.off @22:12:59.80 PCR for ENTap completed -- closed /var/log/esp/ENTap14mar1520h.pcr @22:12:59.85 Cleaning PCR @22:12:59.86 AFV.select :carrierSolution @22:12:59.95 delay 2 seconds @22:13:01.96 AS.reconfigure ASconfig @22:13:02.14 AS.seek 0.500ml @22:13:12.56 delay 1 second @22:13:13.57 AFV.select :holdingCoil @22:13:13.66 ASV.dial :waste1 @22:13:14.14 ATV.dial :air3 @22:13:15.08 AS.reconfigure ASslow1 @22:13:15.11 AS.seek 0.510ml @22:13:17.41 delay 2 seconds @22:13:19.42 ATV.dial :bleach @22:13:19.81 AS.seek 0.810ml @22:14:21.25 delay 3 seconds @22:14:24.26 ATV.dial :air3 @22:14:24.61 delay 3 seconds @22:14:27.67 AS.seek 0.835ml @22:14:33.00 delay 3 seconds @22:14:36.01 ATV.dial :bleach @22:14:36.50 AS.seek 0.820ml @22:14:39.81 delay 3 seconds @22:14:42.82 ARV.dial :waste @22:14:43.42 delay 3 seconds @22:14:46.43 ATV.dial :ARV @22:14:47.18 AS.reconfigure ASconfig @22:14:47.22 AS.seek 0.470ml @22:15:07.90 AFV.select :carrierSolution @22:15:08.00 delay 2 seconds @22:15:10.01 AS.seek 1.000ml @22:15:41.21 delay 1 second @22:15:42.22 AFV.select :holdingCoil @22:15:42.31 delay 1 second @22:15:43.32 AS.reconfigure ASfast @22:15:43.35 AS.seek 0.000ml @22:16:11.06 AFV.select :carrierSolution @22:16:11.15 delay 2 seconds @22:16:13.72 AS.reconfigure ASconfig @22:16:13.76 AS.seek 1.000ml @22:17:12.30 delay 1 second @22:17:13.31 AFV.select :holdingCoil @22:17:13.40 delay 1 second @22:17:14.41 AS.reconfigure ASfast @22:17:14.44 AS.seek 0.000ml @22:17:42.18 AFV.select :carrierSolution @22:17:42.28 delay 2 seconds @22:17:44.29 AS.reconfigure ASconfig @22:17:44.32 AS.seek 1.000ml @22:18:42.94 delay 1 second @22:18:43.95 AFV.select :holdingCoil @22:18:44.04 delay 1 second @22:18:45.05 AS.reconfigure ASfast @22:18:45.08 AS.seek 0.000ml @22:19:12.77 ARV.dial between carrier and air1 @22:19:13.18 ATV.dial :air3 @22:19:14.21 AS.reconfigure ASslow1 @22:19:14.24 AS.seek 0.010ml @22:19:16.58 delay 2 seconds @22:19:18.60 ATV.dial :bleach @22:19:19.08 AS.seek 0.810ml @22:22:02.49 delay 3 seconds @22:22:05.50 ATV.dial :air3 @22:22:05.82 delay 3 seconds @22:22:08.88 AS.seek 0.835ml @22:22:14.26 delay 3 seconds @22:22:17.27 ATV.dial :bleach @22:22:17.67 AS.seek 0.820ml @22:22:20.98 delay 3 seconds @22:22:23.99 ATV.dial PCR @22:22:24.31 AS.reconfigure ASconfig @22:22:24.34 AS.seek empty @22:23:12.44 PCR.program :bleachProfile @22:23:12.50 PCR program should complete in < 2:45 @22:23:12.51 PCR.run 10:00 @22:26:10.70 PCR.off @22:26:10.73 AFV.select :carrierSolution @22:26:10.82 delay 2 seconds @22:26:12.83 AS.seek 1.000ml @22:27:11.47 delay 1 second @22:27:12.48 AFV.select :holdingCoil @22:27:12.57 delay 1 second @22:27:13.58 AS.reconfigure ASfast @22:27:13.61 AS.seek 0.000ml @22:27:41.36 delay 3 seconds @22:27:44.37 AFV.select :carrierSolution @22:27:44.46 delay 2 seconds @22:27:46.47 AS.reconfigure ASconfig @22:27:46.50 AS.seek 1.000ml @22:28:45.09 delay 1 second @22:28:46.10 AFV.select :holdingCoil @22:28:46.19 delay 1 second @22:28:47.20 AS.reconfigure ASfast @22:28:47.23 AS.seek 0.000ml @22:29:14.93 delay 3 seconds @22:29:17.94 AFV.select :carrierSolution @22:29:18.03 delay 2 seconds @22:29:20.04 AS.reconfigure ASconfig @22:29:20.07 AS.seek 1.000ml @22:30:18.66 delay 1 second @22:30:19.67 AFV.select :holdingCoil @22:30:19.76 delay 1 second @22:30:20.77 AS.reconfigure ASfast @22:30:20.80 AS.seek 0.000ml @22:30:48.54 delay 3 seconds @22:30:51.55 AFV.select :carrierSolution @22:30:51.64 delay 2 seconds @22:30:53.65 AS.reconfigure ASconfig @22:30:53.68 AS.seek 1.000ml @22:31:52.25 delay 1 second @22:31:53.26 AFV.select :holdingCoil @22:31:53.35 delay 1 second @22:31:54.36 AS.reconfigure ASfast @22:31:54.39 AS.seek 0.000ml @22:32:22.10 delay 3 seconds @22:32:25.11 AFV.select :carrierSolution @22:32:25.20 delay 2 seconds @22:32:27.21 AS.reconfigure ASconfig @22:32:27.24 AS.seek 1.000ml @22:33:25.83 delay 1 second @22:33:26.84 AFV.select :holdingCoil @22:33:26.93 delay 1 second @22:33:27.94 AS.reconfigure ASnorm @22:33:27.97 AS.seek 0.000ml @22:34:19.42 Setting up PCR for ENTem -- writing /var/log/esp/ENTem14mar1522h.pcr @22:34:19.43 Building PCR Slug from elutionWater with MM2 mix, PP3 primer @22:34:19.44 Priming with carrier @22:34:19.45 ATV.dial ARV @22:34:19.97 ARV.dial :carrier @22:34:20.25 AS.seek 0.500ml @22:34:46.14 AFV.select :carrierSolution @22:34:46.25 delay 2 seconds @22:34:48.26 AS.reconfigure ASconfig @22:34:48.29 AS.seek 1.000ml @22:35:17.73 delay 1 second @22:35:18.74 AFV.select :holdingCoil @22:35:18.84 delay 1 second @22:35:19.85 ATV.dial :waste @22:35:21.10 AS.reconfigure ASnorm @22:35:21.13 AS.seek empty @22:36:12.61 AFV.select :carrierSolution @22:36:12.71 delay 2 seconds @22:36:14.72 AS.reconfigure ASconfig @22:36:14.75 AS.seek 0.500ml @22:36:44.26 delay 1 second @22:36:45.27 AFV.select :holdingCoil @22:36:45.36 Building Slug @22:36:45.37 ATV.dial :ARV @22:36:46.61 delay 2 seconds @22:36:48.62 ARV.dial :air1 @22:36:48.94 delay 2 seconds @22:36:51.00 AS.reconfigure ASslow2 @22:36:51.03 AS.seek 0.506ml @22:36:57.40 delay 2 seconds @22:36:59.41 ARV.dial :MM2 @22:37:00.63 delay 2 seconds @22:37:02.64 Adding MM2 @22:37:02.69 AS.seek 0.524ml @22:37:21.43 delay 25 seconds @22:37:46.44 ARV.dial :PP3 @22:37:46.85 Adding PP3 @22:37:46.87 delay 2 seconds @22:37:48.92 AS.seek 0.530ml @22:37:55.19 delay 10 seconds @22:38:05.20 ARV.dial :air2 @22:38:06.13 adding ARV air @22:38:06.14 delay 2 seconds @22:38:08.20 AS.seek 0.536ml @22:38:14.42 delay 5 seconds @22:38:19.43 ARV.dial :carrier @22:38:20.46 delay 7 seconds @22:38:27.52 AS.seek 0.594ml @22:39:26.81 delay 5 seconds @22:39:31.82 ATV.dial :elutionWater @22:39:32.91 delay 2 seconds @22:39:34.97 AS.seek 0.600ml @22:39:41.32 delay 4 seconds @22:39:45.36 ATV.dial ARV @22:39:46.45 delay 2 seconds @22:39:48.46 ARV.dial :carrier @22:39:48.47 delay 2 seconds @22:39:50.52 AS.seek 0.650ml @22:40:41.65 delay 4 seconds @22:40:45.66 ATV.dial PCR @22:40:46.17 ASV.dial :waste1 @22:40:46.18 ARV.dial :air1 @22:40:46.52 Positioning Slug by 0.324ml @22:40:46.57 AS.seek 0.326ml @22:46:17.39 delay 5 seconds @22:46:22.40 ATV.dial ARV @22:46:22.92 ASV.dial :PCRlock @22:46:23.41 delay 5 seconds @22:46:28.42 Running ENTem ProfileEM60C thermocycling @22:46:28.43 PCR.program ProfileEM60C @22:46:28.54 ProfileEM60C should complete in < 2:11:38 @22:46:28.55 PCR.run 2:49:32.5 @00:41:41.46PDT16-Mar-14 PCR.off @00:41:42.54 PCR for ENTem completed -- closed /var/log/esp/ENTem14mar1522h.pcr @00:41:42.59 Cleaning PCR AFV.select :carrierSolution @00:41:42.68 delay 2 seconds @00:41:44.69 AS.reconfigure ASconfig @00:41:44.72 AS.seek 0.500ml @00:41:55.18 delay 1 second @00:41:56.19 AFV.select :holdingCoil @00:41:56.28 ASV.dial :waste1 @00:41:57.04 ATV.dial :air3 @00:41:58.01 AS.reconfigure ASslow1 @00:41:58.04 AS.seek 0.510ml @00:42:00.30 delay 2 seconds @00:42:02.31 ATV.dial :bleach @00:42:02.70 AS.seek 0.810ml @00:43:04.13 delay 3 seconds @00:43:07.14 ATV.dial :air3 @00:43:07.55 delay 3 seconds @00:43:10.61 AS.seek 0.835ml @00:43:15.96 delay 3 seconds @00:43:18.97 ATV.dial :bleach @00:43:19.37 AS.seek 0.820ml @00:43:22.67 delay 3 seconds @00:43:25.68 ARV.dial :waste @00:43:26.18 delay 3 seconds @00:43:29.19 ATV.dial :ARV @00:43:29.93 AS.reconfigure ASconfig @00:43:29.96 AS.seek 0.470ml @00:43:50.66 AFV.select :carrierSolution @00:43:50.76 delay 2 seconds @00:43:52.77 AS.seek 1.000ml @00:44:24.00 delay 1 second @00:44:25.01 AFV.select :holdingCoil @00:44:25.10 delay 1 second @00:44:26.11 AS.reconfigure ASfast @00:44:26.14 AS.seek 0.000ml @00:44:53.88 AFV.select :carrierSolution @00:44:53.98 delay 2 seconds @00:44:55.99 AS.reconfigure ASconfig @00:44:56.02 AS.seek 1.000ml @00:45:54.62 delay 1 second @00:45:55.63 AFV.select :holdingCoil @00:45:55.72 delay 1 second @00:45:56.73 AS.reconfigure ASfast @00:45:56.77 AS.seek 0.000ml @00:46:24.50 AFV.select :carrierSolution @00:46:24.60 delay 2 seconds @00:46:26.61 AS.reconfigure ASconfig @00:46:26.64 AS.seek 1.000ml @00:47:25.18 delay 1 second @00:47:26.19 AFV.select :holdingCoil @00:47:26.28 delay 1 second @00:47:27.29 AS.reconfigure ASfast @00:47:27.33 AS.seek 0.000ml @00:47:55.06 ARV.dial between carrier and air1 @00:47:55.48 ATV.dial :air3 @00:47:56.42 AS.reconfigure ASslow1 @00:47:56.45 AS.seek 0.010ml @00:47:58.78 delay 2 seconds @00:48:00.79 ATV.dial :bleach @00:48:01.19 AS.seek 0.810ml @00:50:44.60 delay 3 seconds @00:50:47.61 ATV.dial :air3 @00:50:47.96 delay 3 seconds @00:50:51.02 AS.seek 0.835ml @00:50:56.33 delay 3 seconds @00:50:59.34 ATV.dial :bleach @00:50:59.73 AS.seek 0.820ml @00:51:03.02 delay 3 seconds @00:51:06.03 ATV.dial PCR @00:51:06.36 AS.reconfigure ASconfig @00:51:06.39 AS.seek empty @00:51:54.53 PCR.program :bleachProfile @00:51:54.58 PCR program should complete in < 2:45 @00:51:54.59 PCR.run 10:00 @00:54:55.81 PCR.off @00:54:55.84 AFV.select :carrierSolution @00:54:55.93 delay 2 seconds @00:54:57.94 AS.seek 1.000ml @00:55:56.61 delay 1 second @00:55:57.62 AFV.select :holdingCoil @00:55:57.71 delay 1 second @00:55:58.72 AS.reconfigure ASfast @00:55:58.75 AS.seek 0.000ml @00:56:26.49 delay 3 seconds @00:56:29.50 AFV.select :carrierSolution @00:56:29.59 delay 2 seconds @00:56:31.60 AS.reconfigure ASconfig @00:56:31.63 AS.seek 1.000ml @00:57:30.17 delay 1 second @00:57:31.18 AFV.select :holdingCoil @00:57:31.27 delay 1 second @00:57:32.28 AS.reconfigure ASfast @00:57:32.31 AS.seek 0.000ml @00:58:00.03 delay 3 seconds @00:58:03.04 AFV.select :carrierSolution @00:58:03.13 delay 2 seconds @00:58:05.14 AS.reconfigure ASconfig @00:58:05.17 AS.seek 1.000ml @00:59:03.75 delay 1 second @00:59:04.76 AFV.select :holdingCoil @00:59:04.85 delay 1 second @00:59:05.86 AS.reconfigure ASfast @00:59:05.89 AS.seek 0.000ml @00:59:33.62 delay 3 seconds @00:59:36.63 AFV.select :carrierSolution @00:59:36.72 delay 2 seconds @00:59:38.73 AS.reconfigure ASconfig @00:59:38.76 AS.seek 1.000ml @01:00:37.36 delay 1 second @01:00:38.37 AFV.select :holdingCoil @01:00:38.46 delay 1 second @01:00:39.47 AS.reconfigure ASfast @01:00:39.50 AS.seek 0.000ml @01:01:07.21 delay 3 seconds @01:01:10.22 AFV.select :carrierSolution @01:01:10.31 delay 2 seconds @01:01:12.32 AS.reconfigure ASconfig @01:01:12.35 AS.seek 1.000ml @01:02:10.95 delay 1 second @01:02:11.96 AFV.select :holdingCoil @01:02:12.05 delay 1 second @01:02:13.06 AS.reconfigure ASnorm @01:02:13.09 AS.seek 0.000ml @01:03:04.55 Setting up PCR for IPCap -- writing /var/log/esp/IPCap14mar1601h.pcr @01:03:04.56 Building PCR Slug from elutionWater with MM1 mix, PP2 primer @01:03:04.57 Priming with carrier @01:03:04.58 ATV.dial ARV @01:03:05.60 ARV.dial :carrier @01:03:05.89 AS.seek 0.500ml @01:03:31.79 AFV.select :carrierSolution @01:03:31.90 delay 2 seconds @01:03:33.91 AS.reconfigure ASconfig @01:03:33.95 AS.seek 1.000ml @01:04:03.36 delay 1 second @01:04:04.37 AFV.select :holdingCoil @01:04:04.46 delay 1 second @01:04:05.47 ATV.dial :waste @01:04:06.84 AS.reconfigure ASnorm @01:04:06.87 AS.seek empty @01:04:58.32 AFV.select :carrierSolution @01:04:58.43 delay 2 seconds @01:05:00.44 AS.reconfigure ASconfig @01:05:00.47 AS.seek 0.500ml @01:05:29.97 delay 1 second @01:05:30.98 AFV.select :holdingCoil @01:05:31.08 Building Slug @01:05:31.09 ATV.dial :ARV @01:05:32.35 delay 2 seconds @01:05:34.36 ARV.dial :air1 @01:05:34.69 delay 2 seconds @01:05:36.75 AS.reconfigure ASslow2 @01:05:36.79 AS.seek 0.506ml @01:05:43.06 delay 2 seconds @01:05:45.07 ARV.dial :MM1 @01:05:45.56 delay 2 seconds @01:05:47.57 Adding MM1 @01:05:47.63 AS.seek 0.524ml @01:06:06.17 delay 25 seconds @01:06:31.18 ARV.dial :PP2 @01:06:31.49 Adding PP2 @01:06:31.50 delay 2 seconds @01:06:33.55 AS.seek 0.530ml @01:06:39.97 delay 10 seconds @01:06:49.98 ARV.dial :air2 @01:06:50.31 adding ARV air @01:06:50.32 delay 2 seconds @01:06:52.38 AS.seek 0.536ml @01:06:58.69 delay 5 seconds @01:07:03.70 ARV.dial :carrier @01:07:04.83 delay 7 seconds @01:07:11.89 AS.seek 0.594ml @01:08:11.20 delay 5 seconds @01:08:16.21 ATV.dial :elutionWater @01:08:17.30 delay 2 seconds @01:08:19.35 AS.seek 0.600ml @01:08:25.67 delay 4 seconds @01:08:29.68 ATV.dial ARV @01:08:30.85 delay 2 seconds @01:08:32.85 ARV.dial :carrier @01:08:32.86 delay 2 seconds @01:08:34.91 AS.seek 0.650ml @01:09:26.09 delay 4 seconds @01:09:30.10 ATV.dial PCR @01:09:30.62 ASV.dial :waste1 @01:09:30.63 ARV.dial :air1 @01:09:30.97 Positioning Slug by 0.324ml @01:09:31.02 AS.seek 0.326ml @01:15:01.71 delay 5 seconds @01:15:06.72 ATV.dial ARV @01:15:07.32 ASV.dial :PCRlock @01:15:07.76 delay 5 seconds @01:15:12.77 Running IPCap Profile59C thermocycling @01:15:12.78 PCR.program Profile59C @01:15:12.88 Profile59C should complete in < 1:39:08 @01:15:12.89 PCR.run 2:08:55 @02:38:10.71 PCR.off @02:38:11.25 PCR for IPCap completed -- closed /var/log/esp/IPCap14mar1601h.pcr @02:38:11.30 Cleaning PCR AFV.select :carrierSolution @02:38:11.39 delay 2 seconds @02:38:13.40 AS.reconfigure ASconfig @02:38:13.43 AS.seek 0.500ml @02:38:23.86 delay 1 second @02:38:24.87 AFV.select :holdingCoil @02:38:24.96 ASV.dial :waste1 @02:38:25.42 ATV.dial :air3 @02:38:26.36 AS.reconfigure ASslow1 @02:38:26.39 AS.seek 0.510ml @02:38:28.68 delay 2 seconds @02:38:30.69 ATV.dial :bleach @02:38:31.08 AS.seek 0.810ml @02:39:32.51 delay 3 seconds @02:39:35.52 ATV.dial :air3 @02:39:35.87 delay 3 seconds @02:39:38.93 AS.seek 0.835ml @02:39:44.26 delay 3 seconds @02:39:47.27 ATV.dial :bleach @02:39:47.76 AS.seek 0.820ml @02:39:51.07 delay 3 seconds @02:39:54.08 ARV.dial :waste @02:39:54.57 delay 3 seconds @02:39:57.58 ATV.dial :ARV @02:39:58.42 AS.reconfigure ASconfig @02:39:58.45 AS.seek 0.470ml @02:40:19.13 AFV.select :carrierSolution @02:40:19.23 delay 2 seconds @02:40:21.24 AS.seek 1.000ml @02:40:52.44 delay 1 second @02:40:53.45 AFV.select :holdingCoil @02:40:53.54 delay 1 second @02:40:54.55 AS.reconfigure ASfast @02:40:54.58 AS.seek 0.000ml @02:41:22.32 AFV.select :carrierSolution @02:41:22.42 delay 2 seconds @02:41:24.43 AS.reconfigure ASconfig @02:41:24.46 AS.seek 1.000ml @02:42:23.05 delay 1 second @02:42:24.06 AFV.select :holdingCoil @02:42:24.15 delay 1 second @02:42:25.16 AS.reconfigure ASfast @02:42:25.19 AS.seek 0.000ml @02:42:52.93 AFV.select :carrierSolution @02:42:53.03 delay 2 seconds @02:42:55.04 AS.reconfigure ASconfig @02:42:55.07 AS.seek 1.000ml @02:43:53.66 delay 1 second @02:43:54.67 AFV.select :holdingCoil @02:43:54.76 delay 1 second @02:43:55.77 AS.reconfigure ASfast @02:43:55.80 AS.seek 0.000ml @02:44:23.52 ARV.dial between carrier and air1 @02:44:23.94 ATV.dial :air3 @02:44:24.88 AS.reconfigure ASslow1 @02:44:24.92 AS.seek 0.010ml @02:44:27.27 delay 2 seconds @02:44:29.28 ATV.dial :bleach @02:44:29.77 AS.seek 0.810ml @02:47:13.17 delay 3 seconds @02:47:16.18 ATV.dial :air3 @02:47:16.51 delay 3 seconds @02:47:19.57 AS.seek 0.835ml @02:47:24.90 delay 3 seconds @02:47:27.91 ATV.dial :bleach @02:47:28.29 AS.seek 0.820ml @02:47:31.59 delay 3 seconds @02:47:34.60 ATV.dial PCR @02:47:34.93 AS.reconfigure ASconfig @02:47:34.96 AS.seek empty @02:48:23.09 PCR.program :bleachProfile @02:48:23.14 PCR program should complete in < 2:45 @02:48:23.15 PCR.run 10:00 @02:51:24.36 PCR.off @02:51:24.39 AFV.select :carrierSolution @02:51:24.48 delay 2 seconds @02:51:26.49 AS.seek 1.000ml @02:52:25.15 delay 1 second @02:52:26.16 AFV.select :holdingCoil @02:52:26.25 delay 1 second @02:52:27.26 AS.reconfigure ASfast @02:52:27.32 AS.seek 0.000ml @02:52:55.01 delay 3 seconds @02:52:58.02 AFV.select :carrierSolution @02:52:58.11 delay 2 seconds @02:53:00.12 AS.reconfigure ASconfig @02:53:00.15 AS.seek 1.000ml @02:53:58.74 delay 1 second @02:53:59.75 AFV.select :holdingCoil @02:53:59.84 delay 1 second @02:54:00.85 AS.reconfigure ASfast @02:54:00.88 AS.seek 0.000ml @02:54:28.59 delay 3 seconds @02:54:31.60 AFV.select :carrierSolution @02:54:31.69 delay 2 seconds @02:54:33.70 AS.reconfigure ASconfig @02:54:33.73 AS.seek 1.000ml @02:55:32.33 delay 1 second @02:55:33.34 AFV.select :holdingCoil @02:55:33.43 delay 1 second @02:55:34.44 AS.reconfigure ASfast @02:55:34.47 AS.seek 0.000ml @02:56:02.21 delay 3 seconds @02:56:05.22 AFV.select :carrierSolution @02:56:05.31 delay 2 seconds @02:56:07.32 AS.reconfigure ASconfig @02:56:07.35 AS.seek 1.000ml @02:57:05.94 delay 1 second @02:57:06.95 AFV.select :holdingCoil @02:57:07.04 delay 1 second @02:57:08.05 AS.reconfigure ASfast @02:57:08.08 AS.seek 0.000ml @02:57:35.81 delay 3 seconds @02:57:38.82 AFV.select :carrierSolution @02:57:38.91 delay 2 seconds @02:57:40.92 AS.reconfigure ASconfig @02:57:40.95 AS.seek 1.000ml @02:58:39.58 delay 1 second @02:58:40.59 AFV.select :holdingCoil @02:58:40.68 delay 1 second @02:58:41.69 AS.reconfigure ASnorm @02:58:41.72 AS.seek 0.000ml @02:59:33.20 Setting up PCR for IPCem -- writing /var/log/esp/IPCem14mar1602h.pcr @02:59:33.21 Building PCR Slug from elutionWater with MM3 mix, PP5 primer @02:59:33.22 Priming with carrier @02:59:33.23 ATV.dial ARV @02:59:33.75 ARV.dial :carrier @02:59:34.03 AS.seek 0.500ml @02:59:59.92 AFV.select :carrierSolution @03:00:00.03 delay 2 seconds @03:00:02.04 AS.reconfigure ASconfig @03:00:02.08 AS.seek 1.000ml @03:00:31.53 delay 1 second @03:00:32.54 AFV.select :holdingCoil @03:00:32.64 delay 1 second @03:00:33.65 ATV.dial :waste @03:00:34.93 AS.reconfigure ASnorm @03:00:34.96 AS.seek empty @03:01:26.42 AFV.select :carrierSolution @03:01:26.52 delay 2 seconds @03:01:28.53 AS.reconfigure ASconfig @03:01:28.56 AS.seek 0.500ml @03:01:58.04 delay 1 second @03:01:59.05 AFV.select :holdingCoil @03:01:59.14 Building Slug @03:01:59.15 ATV.dial :ARV @03:02:00.41 delay 2 seconds @03:02:02.42 ARV.dial :air1 @03:02:02.74 delay 2 seconds @03:02:04.80 AS.reconfigure ASslow2 @03:02:04.83 AS.seek 0.506ml @03:02:11.20 delay 2 seconds @03:02:13.21 ARV.dial :MM3 @03:02:14.07 delay 2 seconds @03:02:16.08 Adding MM3 @03:02:16.14 AS.seek 0.524ml @03:02:34.82 delay 25 seconds @03:02:59.83 ARV.dial :PP5 @03:03:00.16 Adding PP5 @03:03:00.17 delay 2 seconds @03:03:02.23 AS.seek 0.530ml @03:03:08.48 delay 10 seconds @03:03:18.49 ARV.dial :air3 @03:03:18.81 adding ARV air @03:03:18.82 delay 2 seconds @03:03:20.87 AS.seek 0.536ml @03:03:27.18 delay 5 seconds @03:03:32.19 ARV.dial :carrier @03:03:33.22 delay 7 seconds @03:03:40.28 AS.seek 0.594ml @03:04:39.57 delay 5 seconds @03:04:44.58 ATV.dial :elutionWater @03:04:45.66 delay 2 seconds @03:04:47.72 AS.seek 0.600ml @03:04:54.14 delay 4 seconds @03:04:58.15 ATV.dial ARV @03:04:59.30 delay 2 seconds @03:05:01.31 ARV.dial :carrier @03:05:01.32 delay 2 seconds @03:05:03.37 AS.seek 0.650ml @03:05:54.70 delay 4 seconds @03:05:58.71 ATV.dial PCR @03:05:59.22 ASV.dial :waste1 @03:05:59.23 ARV.dial :air1 @03:05:59.57 Positioning Slug by 0.324ml @03:05:59.62 AS.seek 0.326ml @03:11:30.34 delay 5 seconds @03:11:35.35 ATV.dial ARV @03:11:35.95 ASV.dial :PCRlock @03:11:36.44 delay 5 seconds @03:11:41.45 Running IPCem ProfileEM59C thermocycling @03:11:41.46 PCR.program ProfileEM59C @03:11:41.57 ProfileEM59C should complete in < 2:11:38 @03:11:41.58 PCR.run 2:49:32.5 @05:06:45.41 PCR.off @05:06:45.95 PCR for IPCem completed -- closed /var/log/esp/IPCem14mar1602h.pcr @05:06:46.00 Cleaning PCR AFV.select :carrierSolution @05:06:46.09 delay 2 seconds @05:06:48.10 AS.reconfigure ASconfig @05:06:48.13 AS.seek 0.500ml @05:06:58.54 delay 1 second @05:06:59.55 AFV.select :holdingCoil @05:06:59.64 ASV.dial :waste1 @05:07:00.13 ATV.dial :air3 @05:07:01.07 AS.reconfigure ASslow1 @05:07:01.10 AS.seek 0.510ml @05:07:03.36 delay 2 seconds @05:07:05.37 ATV.dial :bleach @05:07:05.85 AS.seek 0.810ml @05:08:07.27 delay 3 seconds @05:08:10.28 ATV.dial :air3 @05:08:10.61 delay 3 seconds @05:08:13.67 AS.seek 0.835ml @05:08:19.02 delay 3 seconds @05:08:22.03 ATV.dial :bleach @05:08:22.43 AS.seek 0.820ml @05:08:25.75 delay 3 seconds @05:08:28.75 ARV.dial :waste @05:08:29.36 delay 3 seconds @05:08:32.38 ATV.dial :ARV @05:08:33.19 AS.reconfigure ASconfig @05:08:33.23 AS.seek 0.470ml @05:08:53.90 AFV.select :carrierSolution @05:08:53.99 delay 2 seconds @05:08:56.00 AS.seek 1.000ml @05:09:27.21 delay 1 second @05:09:28.22 AFV.select :holdingCoil @05:09:28.31 delay 1 second @05:09:29.32 AS.reconfigure ASfast @05:09:29.35 AS.seek 0.000ml @05:09:57.09 AFV.select :carrierSolution @05:09:57.19 delay 2 seconds @05:09:59.20 AS.reconfigure ASconfig @05:09:59.23 AS.seek 1.000ml @05:10:57.83 delay 1 second @05:10:58.84 AFV.select :holdingCoil @05:10:58.93 delay 1 second @05:10:59.94 AS.reconfigure ASfast @05:10:59.97 AS.seek 0.000ml @05:11:27.71 AFV.select :carrierSolution @05:11:27.81 delay 2 seconds @05:11:29.82 AS.reconfigure ASconfig @05:11:29.85 AS.seek 1.000ml @05:12:28.39 delay 1 second @05:12:29.40 AFV.select :holdingCoil @05:12:29.49 delay 1 second @05:12:30.50 AS.reconfigure ASfast @05:12:30.53 AS.seek 0.000ml @05:12:58.25 ARV.dial between carrier and air1 @05:12:58.74 ATV.dial :air3 @05:12:59.68 AS.reconfigure ASslow1 @05:12:59.72 AS.seek 0.010ml @05:13:02.67 delay 2 seconds @05:13:04.68 ATV.dial :bleach @05:13:05.16 AS.seek 0.810ml @05:15:48.56 delay 3 seconds @05:15:51.57 ATV.dial :air3 @05:15:51.90 delay 3 seconds @05:15:54.96 AS.seek 0.835ml @05:16:00.31 delay 3 seconds @05:16:03.32 ATV.dial :bleach @05:16:03.72 AS.seek 0.820ml @05:16:07.03 delay 3 seconds @05:16:10.04 ATV.dial PCR @05:16:10.37 AS.reconfigure ASconfig @05:16:10.40 AS.seek empty @05:16:58.54 PCR.program :bleachProfile @05:16:58.60 PCR program should complete in < 2:45 @05:16:58.61 PCR.run 10:00 @05:19:59.82 PCR.off @05:19:59.85 AFV.select :carrierSolution @05:19:59.94 delay 2 seconds @05:20:01.95 AS.seek 1.000ml @05:21:00.60 delay 1 second @05:21:01.61 AFV.select :holdingCoil @05:21:01.70 delay 1 second @05:21:02.71 AS.reconfigure ASfast @05:21:02.74 AS.seek 0.000ml @05:21:30.47 delay 3 seconds @05:21:33.48 AFV.select :carrierSolution @05:21:33.57 delay 2 seconds @05:21:35.58 AS.reconfigure ASconfig @05:21:35.61 AS.seek 1.000ml @05:22:34.22 delay 1 second @05:22:35.23 AFV.select :holdingCoil @05:22:35.32 delay 1 second @05:22:36.33 AS.reconfigure ASfast @05:22:36.36 AS.seek 0.000ml @05:23:04.05 delay 3 seconds @05:23:07.06 AFV.select :carrierSolution @05:23:07.15 delay 2 seconds @05:23:09.16 AS.reconfigure ASconfig @05:23:09.19 AS.seek 1.000ml @05:24:07.78 delay 1 second @05:24:08.79 AFV.select :holdingCoil @05:24:08.88 delay 1 second @05:24:09.89 AS.reconfigure ASfast @05:24:09.92 AS.seek 0.000ml @05:24:37.65 delay 3 seconds @05:24:40.66 AFV.select :carrierSolution @05:24:40.75 delay 2 seconds @05:24:42.76 AS.reconfigure ASconfig @05:24:42.79 AS.seek 1.000ml @05:25:41.40 delay 1 second @05:25:42.41 AFV.select :holdingCoil @05:25:42.50 delay 1 second @05:25:43.51 AS.reconfigure ASfast @05:25:43.54 AS.seek 0.000ml @05:26:11.27 delay 3 seconds @05:26:14.28 AFV.select :carrierSolution @05:26:14.37 delay 2 seconds @05:26:16.38 AS.reconfigure ASconfig @05:26:16.41 AS.seek 1.000ml @05:27:15.02 delay 1 second @05:27:16.03 AFV.select :holdingCoil @05:27:16.12 delay 1 second @05:27:17.13 AS.reconfigure ASnorm @05:27:17.16 AS.seek 0.000ml @05:28:08.62 AS.stop @05:28:08.64 CC.stop @05:28:08.67 CS.stop @05:28:08.69 PC.stop @05:28:08.72 PS.stop @05:28:08.75 Forearm.stop @05:28:08.78 Elbow.stop @05:28:08.81 SS.stop @05:28:08.88 Sleepy.power :raw,:OFF @05:28:08.89 Sleepy.power :dwarf,:OFF Shutdown @05:28:08.90 GoodNews.email "MFBPCR completed",Subject => "Shutdown" @03:41:44.42PDT17-Mar-14 Session Begun @03:41:44.44 -> Sleepy.cycleModemPower! 9 @03:41:44.46 Session Ended @08:00:00.33
Powering up Dwarves @08:00:05.43 Sleepy.power :raw,:ON @08:00:05.91 SPE.configure SPEconfig @08:00:05.93 AS.configure ASconfig @08:00:05.97 CC.configure CCconfig @08:00:06.00 CS.configure CSconfig @08:00:06.03 PC.configure PCconfig @08:00:06.06 PS.configure PSconfig @08:00:06.09 Forearm.configure LooseForearm @08:00:06.12 Elbow.configure LooseElbow @08:00:06.15 SC.configure SCloose @08:00:06.18 SE.configure SEconfig @08:00:06.22 SS.configure SSconfig @08:00:06.25 CH.configure CHconfig @08:00:06.34 PH.configure PHconfig @08:00:06.41 Valve.reset @08:00:06.93 PCR.off @08:00:06.96 PCR.pidtemp= 50,0.1,0,300,25,25 @08:00:06.99 PCR.caltemp= 6.6661,0.0344,0.0 @08:00:07.09 Intake.select :close @08:00:07.12 Exhaust.select :close CTV.dial :air @08:00:07.16 PSV.dial :air @08:00:07.19
PC.reconfigure PCconfig @08:00:07.24 PC.home.seek open @08:00:07.33 CSV.dial :waste1 @08:00:07.62 PS.reconfigure PSconfig @08:00:07.65 PS.home.seek 0.000ml @08:00:07.76 SSV.dial :spit,avoiding => exhaust @08:00:08.55 CS.reconfigure CSfast @08:00:08.58 SS.reconfigure SSconfig @08:00:08.67 SS.home.seek 0.00ml @08:00:08.69 CS.home.seek 0.000ml @08:00:08.76 CC.reconfigure CCconfig @08:00:08.80 CC.home.seek open @08:00:17.94 PS.coast @08:00:18.02
CC.seek open @08:00:18.04 PC.seek open @08:00:18.20
Forearm.reconfigure LooseForearm @08:00:18.23 Forearm.home.seek retract @08:00:18.97 Hand.grip @08:00:18.99
Elbow.jog -1500 @08:00:19.49 Elbow.reconfigure LooseElbow @08:00:19.53 Elbow.home.seek clear @08:00:24.29 SE.reconfigure SEconfig @08:00:24.32 SE.home.seek down @08:00:24.90 SC.reconfigure SCsloppy @08:00:24.94 SC.home.seek initial @08:00:28.67 Forearm.seek retract @08:00:28.73 Elbow.seek hammer @08:00:29.89 Forearm.reconfigure TightForearm @08:00:29.94 Forearm.seek 2 @08:00:31.05 Forearm.seek retract @08:00:32.15 Forearm.reconfigure LooseForearm @08:00:32.73PDT17-Mar-14 ===> STARTED wqPCR (phase #13) <=== @08:00:32.74 Starting initialPurge @08:00:32.75 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:00:32.80 Hand.release @08:00:33.67 Elbow.reconfigure TightElbow @08:00:33.71 Elbow.seek FlushPuck::Garage @08:00:35.52 Elbow.reconfigure LooseElbow @08:00:35.60 Forearm.seek garage @08:00:36.55 Hand.grip @08:00:37.43 Forearm.seek retract @08:00:38.41 Elbow.reconfigure TightElbow @08:00:38.45 Elbow.seek CC @08:00:41.27 Elbow.reconfigure LooseElbow @08:00:41.35 Forearm.reconfigure TightForearm @08:00:41.38 Forearm.seek CC @08:00:42.44 CC.seek closed @08:00:46.21 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.96 @08:00:46.27 Hand.release @08:00:47.00 Forearm.reconfigure LooseForearm @08:00:47.03 Forearm.seek retract @08:00:47.97 Flushing the Collection Valve::Manifold @08:00:48.07 CS Pulling 5ml of flush @08:00:48.08 CSV.dial CSR @08:00:49.35 CSR.select :flush @08:00:49.45 CS.reconfigure CSconfig @08:00:49.49 CS.seek 5.000ml @08:01:03.00 delay 5 seconds @08:01:08.02 CSR.select :air @08:01:08.16 CS.reconfigure CSfast @08:01:08.19 CS.seek 7.500ml @08:01:14.75 CSV.dial :waste @08:01:16.02 CS.reconfigure CSconfig @08:01:16.06 CS.seek 0.000ml @08:01:35.88 Purging external Intake @08:01:35.97 CSV.dial :flush @08:01:36.40 CS.reconfigure CSfast @08:01:36.43 CS.seek 10.000ml @08:02:00.35 delay 5 seconds @08:02:05.36 CSV.dial CBV @08:02:05.38
CBV.dial CSV @08:02:06.17 CTV.dial :intake @08:02:06.88 Intake.select :open @08:02:06.97 CS.seek 0.000ml @08:02:31.19 Intake.select :close @08:02:31.28 CTV.dial :air @08:02:32.02 Flushing upper manifold @08:02:32.11 CSV.dial :flush @08:02:32.90 CS.seek 5.000ml @08:02:45.25 delay 5 seconds @08:02:50.27 CSV.dial :air @08:02:50.75 CS.seek 7.000ml @08:02:56.19 CSV.dial CBV @08:02:56.21
CBV.dial CSV @08:02:57.50 CTV.dial :air @08:02:57.52 CS.seek 0.000ml @08:03:14.40 delay 7 seconds @08:03:21.42 CS.seek 3.000ml @08:03:29.12 CSV.dial :waste @08:03:29.55 CS.seek 0.000ml @08:03:37.45 CSV.dial :flush @08:03:37.88 CS.seek 3.000ml @08:03:45.59 delay 5 seconds @08:03:50.61 CSV.dial :air @08:03:51.09 CS.seek 5.000ml @08:03:56.53 CSV.dial :air @08:03:56.55 CS.seek 0.000ml @08:04:08.87 delay 7 seconds @08:04:15.88 CS.seek 3.000ml @08:04:23.65 CSV.dial :waste @08:04:24.46 CS.seek 0.000ml @08:04:32.24 Garaging FlushPuck in Collection Clamp @08:04:32.30 Elbow.reconfigure TightElbow @08:04:32.33 Elbow.seek CC @08:04:32.56 Elbow.reconfigure LooseElbow @08:04:32.65 Forearm.seek CC @08:04:33.63 CC.seek sealed @08:04:34.56 Hand.grip @08:04:35.33 CC.seek open @08:04:38.62 Forearm.seek retract @08:04:39.57 Elbow.reconfigure TightElbow @08:04:39.61 Elbow.seek garage @08:04:42.37 Elbow.reconfigure LooseElbow @08:04:43.01 Forearm.reconfigure TightForearm @08:04:43.04 Forearm.seek garage @08:04:44.21 Hand.release @08:04:44.99 Forearm.reconfigure LooseForearm @08:04:45.02 Forearm.seek retract @08:04:46.10 Starting mfb @08:04:46.11 pcrBAC sampling at most 1000ml, wcr at most 1000ml @08:04:46.13 Loading sh1 puck from tube 3 into Collection Clamp @08:04:46.16 BACfans.email "Can@08:00:32, 15.7C, 70% humidity, 14.2psia, 13.709V, 0.598A, 0.610A avg, 8.19W\nCTD data unavailable\nISUS data unavailable",Subject => "pcrBAC sampling at most 1000ml, wcr at most 1000ml" @08:04:47.94 Readying the MFB @08:04:47.95 Homing MFB Syringe @08:04:47.97 AFV.select :holdingCoil @08:04:48.10
SC.reconfigure SCtight @08:04:48.15 SC.seek 3 @08:04:48.22 ATV.dial :air2 @08:04:49.52 AS.reconfigure ASconfig @08:04:49.57 AS.home.seek home @08:04:50.40
SC.reconfigure SCloose @08:04:50.58 Elbow.reconfigure TightElbow @08:04:50.63 Elbow.seek 3 @08:04:52.51 Forearm.reconfigure TightForearm @08:04:52.54 Forearm.seek 3 + 800 counts @08:04:53.63 SE.reconfigure SEpick @08:04:53.68 SE.seek beamBreak @08:04:55.89 SE.configure SEconfig @08:04:56.02 Elbow.seek 3 @08:04:56.18 Forearm.seek 3 + 300 counts @08:04:56.40 Forearm.reconfigure LooseForearm @08:04:56.44 Hand.grip @08:04:57.06 AFV.select :carrierSolution @08:04:57.16 delay 2 seconds @08:04:57.21 SE.seek down @08:04:57.35
Forearm.seek retract @08:04:58.33 Elbow.seek CC @08:04:59.17 AS.seek 1.000ml @08:04:59.32 SE.coast @08:04:59.84
Elbow.reconfigure LooseElbow @08:04:59.92 Forearm.reconfigure TightForearm @08:04:59.95 Forearm.seek CC @08:05:01.04 CC.seek closed @08:05:04.77 Collection Clamp onSpring/offSpring for 5/22 samples, on/off torque 1.97 @08:05:04.78 Hand.release @08:05:05.49 Forearm.reconfigure LooseForearm @08:05:05.52 Forearm.seek retract @08:05:06.47 SC.coast @08:05:06.50 collectSample 1000ml w/bubble point of 55psi @08:05:06.51 Priming 7ml @08:05:06.52 Sleepy.power :sampler,:ON @08:05:06.53 Intake.select :close Exhaust.select :close @08:05:06.54 SS.hold @08:05:06.69 Calibrating pressure and temperature sensors @08:05:06.71 CTV.dial :air SSV.dial :spit,avoiding => exhaust @08:05:06.73 SS.reconfigure SSfast @08:05:06.76 SS.seek 5.00ml @08:05:09.55 SSV.dial CBV @08:05:09.57
CBV.dial SSV @08:05:10.94 delay 3.8 seconds @08:05:14.82 SSV.dial :spit @08:05:16.17 SS.seek 0.00ml @08:05:18.74 SSV.dial CBV @08:05:20.10 CTV.dial :intake @08:05:20.85 Waiting for external sampler to finish priming... @08:05:20.86 delayUntil "08:15:06.52" @08:05:50.85 delay 1 second @08:05:51.86 AFV.select :holdingCoil @08:05:51.95 delay 1 second @08:05:52.96 ATV.dial :waste @08:05:53.88 AS.reconfigure ASnorm @08:05:53.91 AS.home.seek empty @08:06:47.66 ATV.dial :waste @08:06:47.67 ARV.dial between carrier and air1 @08:06:47.74 ASV.dial :waste1 @08:15:06.53 SSV.dial CBV @08:15:06.55
CBV.dial SSV @08:15:06.56 Allowing 55s for 7.0ml of remaining 7.0ml Intake.select :open @08:15:08.69 Intake @37.4psia, vacuum from 20.0 to 24.0psi @08:15:08.70 SS.reconfigure forSampling @08:15:08.74 SS.seek 7.00ml @08:15:14.38 Stroke completed -- waiting for pressure to equalize ... @08:15:15.02 Intake.select :close @08:15:15.16 SSV.dial :exhaust @08:15:15.94 Exhaust.select :open @08:15:16.03 SS.reconfigure SSexhale @08:15:16.07 SS.seek 0.00ml @08:15:19.78 Exhaust.select :close @08:15:19.88 Sampling 1000.0ml @08:15:19.89 Intake.select :close @08:15:19.90 Exhaust.select :close @08:15:19.91 SS.reconfigure SSconfig @08:15:19.94 SS.hold @08:15:20.09 CTV.dial :intake @08:15:20.10 SSV.dial CBV @08:15:20.12
CBV.dial SSV @08:15:20.93 Allowing 185s for 25.0ml of remaining 1000.0ml Intake.select :open @08:15:23.06 Intake @35.5psia, vacuum from 20.0 to 24.0psi @08:15:23.07 SS.reconfigure forSampling @08:15:23.11 SS.seek 25.00ml @08:15:37.48 Stroke completed -- waiting for pressure to equalize ... @08:15:38.12 Intake.select :close @08:15:38.25 SSV.dial :exhaust @08:15:39.05 Exhaust.select :open @08:15:39.14 SS.reconfigure SSexhale @08:15:39.18 SS.seek 0.00ml @08:15:48.12 Exhaust.select :close @08:15:48.21 SSV.dial CBV @08:15:48.23
CBV.dial SSV @08:15:49.05 Allowing 185s for 25.0ml of remaining 975.0ml @08:15:49.06 Intake.select :open @08:15:51.20 Intake @34.5psia, vacuum from 20.0 to 24.0psi @08:15:51.21 SS.reconfigure forSampling @08:15:51.24 SS.seek 25.00ml @08:16:05.65 Stroke completed -- waiting for pressure to equalize ... @08:16:06.29 Intake.select :close @08:16:06.43 SSV.dial :exhaust @08:16:07.23 Exhaust.select :open @08:16:07.32 SS.reconfigure SSexhale @08:16:07.36 SS.seek 0.00ml @08:16:16.29 Exhaust.select :close @08:16:16.38 SSV.dial CBV @08:16:16.40
CBV.dial SSV @08:16:17.20 Allowing 185s for 25.0ml of remaining 950.0ml @08:16:17.21 Intake.select :open @08:16:19.34 Intake @34.5psia, vacuum from 20.0 to 24.0psi @08:16:19.35 SS.reconfigure forSampling @08:16:19.39 SS.seek 25.00ml @08:16:33.87 Stroke completed -- waiting for pressure to equalize ... @08:16:34.51 Intake.select :close @08:16:34.64 SSV.dial :exhaust @08:16:35.45 Exhaust.select :open @08:16:35.54 SS.reconfigure SSexhale @08:16:35.58 SS.seek 0.00ml @08:16:44.39 Exhaust.select :close @08:16:44.48 SSV.dial CBV @08:16:44.50
CBV.dial SSV @08:16:45.31 Allowing 185s for 25.0ml of remaining 925.0ml @08:16:45.32 Intake.select :open @08:16:47.45 Intake @34.0psia, vacuum from 20.0 to 24.0psi @08:16:47.46 SS.reconfigure forSampling @08:16:47.50 SS.seek 25.00ml @08:17:00.01 Vacuum too high -- waiting up to 40s @08:17:00.28 Intake @26.3psia, vacuum from 19.8 to 23.8psi @08:17:00.29 SS.reconfigure forSampling @08:17:00.32 SS.seek 25.00ml @08:17:02.57 Stroke completed -- waiting for pressure to equalize ... @08:17:03.21 Intake.select :close @08:17:03.34 SSV.dial :exhaust @08:17:04.14 Exhaust.select :open @08:17:04.23 SS.reconfigure SSexhale @08:17:04.27 SS.seek 0.00ml @08:17:13.29 Exhaust.select :close @08:17:13.38 SSV.dial CBV @08:17:13.40
CBV.dial SSV @08:17:14.21 Allowing 185s for 25.0ml of remaining 900.0ml @08:17:14.22 Intake.select :open @08:17:16.35 Intake @34.3psia, vacuum from 20.0 to 24.0psi @08:17:16.36 SS.reconfigure forSampling @08:17:16.40 SS.seek 25.00ml @08:17:24.35 Vacuum too high -- waiting up to 40s @08:17:24.61 Intake @27.3psia, vacuum from 20.0 to 24.0psi @08:17:24.62 SS.reconfigure forSampling @08:17:24.66 SS.seek 25.00ml @08:17:33.40 Stroke completed -- waiting for pressure to equalize ... @08:17:34.03 Intake.select :close @08:17:34.17 SSV.dial :exhaust @08:17:34.98 Exhaust.select :open @08:17:35.07 SS.reconfigure SSexhale @08:17:35.11 SS.seek 0.00ml @08:17:44.46 Exhaust.select :close @08:17:44.55 SSV.dial CBV @08:17:44.57
CBV.dial SSV @08:17:45.38 Allowing 185s for 25.0ml of remaining 875.0ml @08:17:45.39 Intake.select :open @08:17:47.52 Intake @35.9psia, vacuum from 20.0 to 24.0psi @08:17:47.53 SS.reconfigure forSampling @08:17:47.57 SS.seek 25.00ml @08:17:51.16 Vacuum too high -- waiting up to 40s @08:17:51.42 Intake @29.9psia, vacuum from 20.0 to 24.0psi @08:17:51.43 SS.reconfigure forSampling @08:17:51.47 SS.seek 25.00ml @08:18:06.94 Stroke completed -- waiting for pressure to equalize ... @08:18:07.58 Intake.select :close @08:18:08.20 SSV.dial :exhaust @08:18:09.01 Exhaust.select :open @08:18:09.11 SS.reconfigure SSexhale @08:18:09.15 SS.seek 0.00ml @08:18:18.12 Exhaust.select :close @08:18:18.21 SSV.dial CBV @08:18:18.23
CBV.dial SSV @08:18:19.04 Allowing 185s for 25.0ml of remaining 850.0ml @08:18:19.05 Intake.select :open @08:18:21.18 Intake @38.2psia, vacuum from 20.0 to 24.0psi @08:18:21.20 SS.reconfigure forSampling @08:18:21.23 SS.seek 25.00ml @08:18:24.20 Vacuum too high -- waiting up to 40s @08:18:24.46 Intake @31.8psia, vacuum from 20.0 to 24.0psi @08:18:24.47 SS.reconfigure forSampling @08:18:24.50 SS.seek 25.00ml @08:18:38.97 Vacuum too high -- waiting up to 40s @08:18:39.24 Intake @30.4psia, vacuum from 20.0 to 24.0psi @08:18:39.25 SS.reconfigure forSampling @08:18:39.28 SS.seek 25.00ml @08:18:45.05 Stroke completed -- waiting for pressure to equalize ... @08:18:45.69 Intake.select :close @08:18:45.82 SSV.dial :exhaust @08:18:46.61 Exhaust.select :open @08:18:46.71 SS.reconfigure SSexhale @08:18:46.75 SS.seek 0.00ml @08:18:55.54 Exhaust.select :close @08:18:55.63 SSV.dial CBV @08:18:55.65
CBV.dial SSV @08:18:56.46 Allowing 185s for 25.0ml of remaining 825.0ml @08:18:56.47 Intake.select :open @08:18:58.60 Intake @35.3psia, vacuum from 20.0 to 24.0psi @08:18:58.61 SS.reconfigure forSampling @08:18:58.65 SS.seek 25.00ml @08:19:01.81 Vacuum too high -- waiting up to 40s @08:19:03.06 Intake @35.0psia, vacuum from 20.0 to 24.0psi @08:19:03.07 SS.reconfigure forSampling @08:19:03.11 SS.seek 25.00ml @08:19:16.69 Vacuum too high -- waiting up to 40s @08:19:16.96 Intake @31.6psia, vacuum from 20.0 to 24.0psi @08:19:16.97 SS.reconfigure forSampling @08:19:17.00 SS.seek 25.00ml @08:19:35.99 Stroke completed -- waiting for pressure to equalize ... @08:19:36.62 Intake.select :close @08:19:36.75 SSV.dial :exhaust @08:19:37.55 Exhaust.select :open @08:19:37.64 SS.reconfigure SSexhale @08:19:37.68 SS.seek 0.00ml @08:19:46.53 Exhaust.select :close @08:19:46.62 SSV.dial CBV @08:19:46.64
CBV.dial SSV @08:19:47.46 Allowing 185s for 25.0ml of remaining 800.0ml @08:19:47.47 Intake.select :open @08:19:49.60 Intake @35.8psia, vacuum from 20.0 to 24.0psi @08:19:49.61 SS.reconfigure forSampling @08:19:49.65 SS.seek 25.00ml @08:20:15.90 Vacuum too high -- waiting up to 40s @08:20:16.16 Intake @33.1psia, vacuum from 20.0 to 24.0psi @08:20:16.17 SS.reconfigure forSampling @08:20:16.21 SS.seek 25.00ml @08:20:37.02 Stroke completed -- waiting for pressure to equalize ... @08:20:37.65 Intake.select :close @08:20:37.79 SSV.dial :exhaust @08:20:38.59 Exhaust.select :open @08:20:38.68 SS.reconfigure SSexhale @08:20:38.72 SS.seek 0.00ml @08:20:47.59 Exhaust.select :close @08:20:47.68 SSV.dial CBV @08:20:47.70
CBV.dial SSV @08:20:48.51 Allowing 185s for 25.0ml of remaining 775.0ml @08:20:48.52 Intake.select :open @08:20:50.65 Intake @34.7psia, vacuum from 20.0 to 24.0psi @08:20:50.66 SS.reconfigure forSampling @08:20:50.70 SS.seek 25.00ml @08:21:40.99 Vacuum too high -- waiting up to 40s @08:21:41.26 Intake @32.8psia, vacuum from 20.0 to 24.0psi @08:21:41.27 SS.reconfigure forSampling @08:21:41.30 SS.seek 25.00ml @08:21:58.54 Stroke completed -- waiting for pressure to equalize ... @08:21:59.75 Intake.select :close @08:21:59.89 SSV.dial :exhaust @08:22:00.68 Exhaust.select :open @08:22:00.77 SS.reconfigure SSexhale @08:22:00.81 SS.seek 0.00ml @08:22:09.65 Exhaust.select :close @08:22:09.74 SSV.dial CBV @08:22:09.76
CBV.dial SSV @08:22:10.57 Allowing 185s for 25.0ml of remaining 750.0ml @08:22:10.58 Intake.select :open @08:22:12.71 Intake @33.3psia, vacuum from 20.0 to 24.0psi @08:22:12.72 SS.reconfigure forSampling @08:22:12.76 SS.seek 25.00ml @08:24:03.25 Stroke completed -- waiting for pressure to equalize ... @08:24:04.46 Intake.select :close @08:24:04.60 SSV.dial :exhaust @08:24:05.40 Exhaust.select :open @08:24:05.49 SS.reconfigure SSexhale @08:24:05.53 SS.seek 0.00ml @08:24:14.25 Exhaust.select :close @08:24:14.34 SSV.dial CBV @08:24:14.36
CBV.dial SSV @08:24:15.18 Allowing 185s for 25.0ml of remaining 725.0ml @08:24:15.19 Intake.select :open @08:24:17.32 Intake @37.1psia, vacuum from 20.0 to 24.0psi @08:24:17.33 SS.reconfigure forSampling @08:24:17.37 SS.seek 25.00ml @08:25:57.21 Vacuum too high -- waiting up to 40s @08:25:57.47 Intake @29.4psia, vacuum from 20.0 to 24.0psi @08:25:57.48 SS.reconfigure forSampling @08:25:57.52 SS.seek 25.00ml @08:26:08.35 Stroke completed -- waiting for pressure to equalize ... @08:26:10.07 Intake.select :close @08:26:10.20 SSV.dial :exhaust @08:26:11.01 Exhaust.select :open @08:26:11.11 SS.reconfigure SSexhale @08:26:11.15 SS.seek 0.00ml @08:26:19.79 Exhaust.select :close @08:26:19.88 SSV.dial CBV @08:26:19.90
CBV.dial SSV @08:26:20.71 Allowing 185s for 25.0ml of remaining 700.0ml @08:26:20.72 Intake.select :open @08:26:22.85 Intake @35.0psia, vacuum from 20.0 to 24.0psi @08:26:22.86 SS.reconfigure forSampling @08:26:22.90 SS.seek 25.00ml @08:26:44.71 Vacuum too high -- waiting up to 40s @08:26:44.98 Intake @30.1psia, vacuum from 20.0 to 24.0psi @08:26:44.99 SS.reconfigure forSampling @08:26:45.03 SS.seek 25.00ml @08:27:51.42 Vacuum too high -- waiting up to 40s @08:27:51.90 Intake @28.6psia, vacuum from 20.0 to 24.0psi @08:27:51.91 SS.reconfigure forSampling @08:27:51.94 SS.seek 25.00ml @08:28:14.53 Stroke completed -- waiting for pressure to equalize ... @08:28:16.92 Intake.select :close @08:28:17.05 SSV.dial :exhaust @08:28:17.85 Exhaust.select :open @08:28:17.95 SS.reconfigure SSexhale @08:28:17.99 SS.seek 0.00ml @08:28:26.66 Exhaust.select :close @08:28:26.75 SSV.dial CBV @08:28:26.77
CBV.dial SSV @08:28:27.59 Allowing 185s for 25.0ml of remaining 675.0ml @08:28:27.60 Intake.select :open @08:28:29.73 Intake @34.7psia, vacuum from 20.0 to 24.0psi @08:28:29.74 SS.reconfigure forSampling @08:28:29.78 SS.seek 25.00ml @08:28:34.11 Vacuum too high -- waiting up to 40s @08:28:34.38 Intake @33.1psia, vacuum from 20.0 to 24.0psi @08:28:34.39 SS.reconfigure forSampling @08:28:34.42 SS.seek 25.00ml @08:28:37.98 Vacuum too high -- waiting up to 40s @08:28:38.25 Intake @31.9psia, vacuum from 20.0 to 24.0psi @08:28:38.26 SS.reconfigure forSampling @08:28:38.29 SS.seek 25.00ml @08:29:09.45 Vacuum too high -- waiting up to 40s @08:29:09.93 Intake @31.5psia, vacuum from 20.0 to 24.0psi @08:29:09.94 SS.reconfigure forSampling @08:29:09.97 SS.seek 25.00ml @08:29:13.45 Vacuum too high -- waiting up to 40s @08:29:13.94 Intake @30.9psia, vacuum from 20.0 to 24.0psi @08:29:13.95 SS.reconfigure forSampling @08:29:13.98 SS.seek 25.00ml @08:29:28.45 Vacuum too high -- waiting up to 40s @08:29:28.94 Intake @30.7psia, vacuum from 20.0 to 24.0psi @08:29:28.95 SS.reconfigure forSampling @08:29:28.99 SS.seek 25.00ml @08:29:31.64 Vacuum too high -- waiting up to 40s @08:29:32.11 Intake @30.4psia, vacuum from 20.0 to 24.0psi @08:29:32.12 SS.reconfigure forSampling @08:29:32.15 SS.seek 25.00ml @08:29:35.29 Vacuum too high -- waiting up to 40s @08:29:35.97 Intake @31.1psia, vacuum from 20.0 to 24.0psi @08:29:35.98 SS.reconfigure forSampling @08:29:36.02 SS.seek 25.00ml @08:29:40.95 Vacuum too high -- waiting up to 40s @08:29:41.43 Intake @30.9psia, vacuum from 20.0 to 24.0psi @08:29:41.44 SS.reconfigure forSampling @08:29:41.48 SS.seek 25.00ml @08:29:44.70 Vacuum too high -- waiting up to 40s @08:29:45.17 Intake @31.5psia, vacuum from 20.0 to 24.0psi @08:29:45.18 SS.reconfigure forSampling @08:29:45.22 SS.seek 25.00ml @08:29:47.46 Vacuum too high -- waiting up to 40s @08:29:47.94 Intake @30.9psia, vacuum from 20.0 to 24.0psi @08:29:47.95 SS.reconfigure forSampling @08:29:47.98 SS.seek 25.00ml @08:29:50.89 Vacuum too high -- waiting up to 40s @08:29:51.56 Intake @31.0psia, vacuum from 20.0 to 24.0psi @08:29:51.57 SS.reconfigure forSampling @08:29:51.61 SS.seek 25.00ml @08:29:59.79 Vacuum too high -- waiting up to 40s @08:30:00.26 Intake @27.3psia, vacuum from 20.0 to 24.0psi @08:30:00.27 SS.reconfigure forSampling @08:30:00.31 SS.seek 25.00ml @08:30:05.60 Vacuum too high -- waiting up to 40s @08:30:06.83 Intake @25.8psia, vacuum from 19.3 to 23.3psi @08:30:06.84 SS.reconfigure forSampling @08:30:06.88 SS.seek 25.00ml @08:30:30.15 Stroke completed -- waiting for pressure to equalize ... @08:30:33.73 Intake.select :close @08:30:33.86 SSV.dial :exhaust @08:30:34.67 Exhaust.select :open @08:30:34.76 SS.reconfigure SSexhale @08:30:34.80 SS.seek 0.00ml @08:30:43.63 Exhaust.select :close @08:30:43.72 SSV.dial CBV @08:30:43.74
CBV.dial SSV @08:30:44.56 Allowing 185s for 25.0ml of remaining 650.0ml @08:30:44.57 Intake.select :open @08:30:46.70 Intake @31.1psia, vacuum from 20.0 to 24.0psi @08:30:46.71 SS.reconfigure forSampling @08:30:46.75 SS.seek 25.00ml @08:30:51.13 Vacuum too high -- waiting up to 40s @08:30:51.62 Intake @30.6psia, vacuum from 20.0 to 24.0psi @08:30:51.63 SS.reconfigure forSampling @08:30:51.66 SS.seek 25.00ml @08:30:54.26 Vacuum too high -- waiting up to 40s @08:30:55.03 Intake @30.3psia, vacuum from 20.0 to 24.0psi @08:30:55.04 SS.reconfigure forSampling @08:30:55.08 SS.seek 25.00ml @08:31:25.22 Vacuum too high -- waiting up to 40s @08:31:26.12 Intake @33.4psia, vacuum from 20.0 to 24.0psi @08:31:26.13 SS.reconfigure forSampling @08:31:26.16 SS.seek 25.00ml @08:31:28.08 Vacuum too high -- waiting up to 40s @08:31:28.55 Intake @33.3psia, vacuum from 20.0 to 24.0psi @08:31:28.56 SS.reconfigure forSampling @08:31:28.59 SS.seek 25.00ml @08:31:30.32 Vacuum too high -- waiting up to 40s @08:31:30.79 Intake @33.5psia, vacuum from 20.0 to 24.0psi @08:31:30.80 SS.reconfigure forSampling @08:31:30.83 SS.seek 25.00ml @08:31:32.42 Vacuum too high -- waiting up to 40s @08:31:32.90 Intake @33.1psia, vacuum from 20.0 to 24.0psi @08:31:32.91 SS.reconfigure forSampling @08:31:32.94 SS.seek 25.00ml @08:31:34.92 Vacuum too high -- waiting up to 40s @08:31:35.61 Intake @33.6psia, vacuum from 20.0 to 24.0psi @08:31:35.62 SS.reconfigure forSampling @08:31:35.65 SS.seek 25.00ml @08:31:37.79 Vacuum too high -- waiting up to 40s @08:31:38.27 Intake @34.1psia, vacuum from 20.0 to 24.0psi @08:31:38.28 SS.reconfigure forSampling @08:31:38.31 SS.seek 25.00ml @08:31:39.89 Vacuum too high -- waiting up to 40s @08:31:40.37 SS.seek 25.00ml @08:31:41.86 Vacuum too high -- waiting up to 40s @08:31:42.32 Intake @34.3psia, vacuum from 20.0 to 24.0psi @08:31:42.33 SS.reconfigure forSampling @08:31:42.37 SS.seek 25.00ml @08:31:43.98 Vacuum too high -- waiting up to 40s @08:31:44.45 Intake @34.3psia, vacuum from 20.0 to 24.0psi @08:31:44.47 SS.reconfigure forSampling @08:31:44.50 SS.seek 25.00ml @08:31:46.11 Vacuum too high -- waiting up to 40s @08:31:46.60 Intake @33.6psia, vacuum from 20.0 to 24.0psi @08:31:46.61 SS.reconfigure forSampling @08:31:46.64 SS.seek 25.00ml @08:31:48.26 Vacuum too high -- waiting up to 40s @08:31:48.73 Intake @33.3psia, vacuum from 20.0 to 24.0psi @08:31:48.74 SS.reconfigure forSampling @08:31:48.77 SS.seek 25.00ml @08:31:50.32 Vacuum too high -- waiting up to 40s @08:31:50.80 Intake @32.5psia, vacuum from 20.0 to 24.0psi @08:31:50.81 SS.reconfigure forSampling @08:31:50.84 SS.seek 25.00ml @08:31:52.82 Vacuum too high -- waiting up to 40s @08:31:53.51 Intake @34.1psia, vacuum from 20.0 to 24.0psi @08:31:53.52 SS.reconfigure forSampling @08:31:53.55 SS.seek 25.00ml @08:31:55.73 Vacuum too high -- waiting up to 40s @08:31:56.00 Intake @33.3psia, vacuum from 20.0 to 24.0psi @08:31:56.01 SS.reconfigure forSampling @08:31:56.05 SS.seek 25.00ml @08:31:57.95 Vacuum too high -- waiting up to 40s @08:31:58.42 Intake @32.9psia, vacuum from 20.0 to 24.0psi @08:31:58.43 SS.reconfigure forSampling @08:31:58.47 SS.seek 25.00ml @08:32:00.23 Vacuum too high -- waiting up to 40s @08:32:00.71 Intake @32.7psia, vacuum from 20.0 to 24.0psi @08:32:00.72 SS.reconfigure forSampling @08:32:00.75 SS.seek 25.00ml @08:32:02.42 Vacuum too high -- waiting up to 40s @08:32:03.11 Intake @32.9psia, vacuum from 20.0 to 24.0psi @08:32:03.12 SS.reconfigure forSampling @08:32:03.15 SS.seek 25.00ml @08:32:04.82 Vacuum too high -- waiting up to 40s @08:32:05.50 Intake @32.8psia, vacuum from 20.0 to 24.0psi @08:32:05.51 SS.reconfigure forSampling @08:32:05.55 SS.seek 25.00ml @08:32:07.26 Vacuum too high -- waiting up to 40s @08:32:07.95 Intake @32.5psia, vacuum from 20.0 to 24.0psi @08:32:07.96 SS.reconfigure forSampling @08:32:07.99 SS.seek 25.00ml @08:32:09.98 Vacuum too high -- waiting up to 40s @08:32:10.68 Intake @33.5psia, vacuum from 20.0 to 24.0psi @08:32:10.69 SS.reconfigure forSampling @08:32:10.72 SS.seek 25.00ml @08:32:12.39 Vacuum too high -- waiting up to 40s @08:32:13.08 Intake @33.3psia, vacuum from 20.0 to 24.0psi @08:32:13.09 SS.reconfigure forSampling @08:32:13.13 SS.seek 25.00ml @08:32:14.89 Vacuum too high -- waiting up to 40s @08:32:15.59 Intake @33.0psia, vacuum from 20.0 to 24.0psi @08:32:15.60 SS.reconfigure forSampling @08:32:15.63 SS.seek 25.00ml @08:32:17.48 Vacuum too high -- waiting up to 40s @08:32:18.17 Intake @32.8psia, vacuum from 20.0 to 24.0psi @08:32:18.18 SS.reconfigure forSampling @08:32:18.21 SS.seek 25.00ml @08:32:19.92 Vacuum too high -- waiting up to 40s @08:32:20.62 Intake @32.9psia, vacuum from 20.0 to 24.0psi @08:32:20.63 SS.reconfigure forSampling @08:32:20.66 SS.seek 25.00ml @08:32:22.42 Vacuum too high -- waiting up to 40s @08:32:23.11 Intake @33.3psia, vacuum from 20.0 to 24.0psi @08:32:23.12 SS.reconfigure forSampling @08:32:23.15 SS.seek 25.00ml @08:32:24.63 Vacuum too high -- waiting up to 40s @08:32:25.33 Intake @34.6psia, vacuum from 20.0 to 24.0psi @08:32:25.34 SS.reconfigure forSampling @08:32:25.37 SS.seek 25.00ml @08:32:26.73 Vacuum too high -- waiting up to 40s @08:32:27.21 Intake @31.4psia, vacuum from 20.0 to 24.0psi @08:32:27.22 SS.reconfigure forSampling @08:32:27.25 SS.seek 25.00ml @08:32:29.13 Vacuum too high -- waiting up to 40s @08:32:29.83 Intake @30.7psia, vacuum from 20.0 to 24.0psi @08:32:29.84 SS.reconfigure forSampling @08:32:29.87 SS.seek 25.00ml @08:32:31.85 Vacuum too high -- waiting up to 40s @08:32:32.74 Intake @30.3psia, vacuum from 20.0 to 24.0psi @08:32:32.75 SS.reconfigure forSampling @08:32:32.78 SS.seek 25.00ml @08:32:35.29 Vacuum too high -- waiting up to 40s @08:32:36.17 Intake @30.1psia, vacuum from 20.0 to 24.0psi @08:32:36.18 SS.reconfigure forSampling @08:32:36.22 SS.seek 25.00ml @08:32:39.69 Vacuum too high -- waiting up to 40s @08:32:40.82 Intake @32.3psia, vacuum from 20.0 to 24.0psi @08:32:40.83 SS.reconfigure forSampling @08:32:40.86 SS.seek 25.00ml @08:32:42.63 Vacuum too high -- waiting up to 40s @08:32:43.33 Intake @32.6psia, vacuum from 20.0 to 24.0psi @08:32:43.34 SS.reconfigure forSampling @08:32:43.38 SS.seek 25.00ml @08:32:45.01 Vacuum too high -- waiting up to 40s @08:32:45.70 Intake @32.1psia, vacuum from 20.0 to 24.0psi @08:32:45.71 SS.reconfigure forSampling @08:32:45.75 SS.seek 25.00ml @08:32:47.13 Vacuum too high -- waiting up to 40s @08:32:47.39 Intake @28.0psia, vacuum from 20.0 to 24.0psi @08:32:47.40 SS.reconfigure forSampling @08:32:47.43 SS.seek 25.00ml @08:32:51.28 Vacuum too high -- waiting up to 40s @08:32:52.81 Intake @27.8psia, vacuum from 20.0 to 24.0psi @08:32:52.82 SS.reconfigure forSampling @08:32:52.86 SS.seek 25.00ml @08:33:10.75 Stroke completed -- waiting for pressure to equalize ... @08:33:15.45 Intake.select :close @08:33:15.59 SSV.dial :exhaust @08:33:16.39 Exhaust.select :open @08:33:16.49 SS.reconfigure SSexhale @08:33:16.53 SS.seek 0.00ml @08:33:25.30 Exhaust.select :close @08:33:25.39 SSV.dial CBV @08:33:25.41
CBV.dial SSV @08:33:26.22 Allowing 185s for 25.0ml of remaining 625.0ml @08:33:26.23 Intake.select :open @08:33:28.36 Intake @34.5psia, vacuum from 20.0 to 24.0psi @08:33:28.37 SS.reconfigure forSampling @08:33:28.41 SS.seek 25.00ml @08:33:31.01 Vacuum too high -- waiting up to 40s @08:33:31.28 Intake @30.7psia, vacuum from 20.0 to 24.0psi @08:33:31.29 SS.reconfigure forSampling @08:33:31.33 SS.seek 25.00ml @08:33:33.38 Vacuum too high -- waiting up to 40s @08:33:34.07 Intake @30.8psia, vacuum from 20.0 to 24.0psi @08:33:34.08 SS.reconfigure forSampling @08:33:34.11 SS.seek 25.00ml @08:33:35.85 Vacuum too high -- waiting up to 40s @08:33:36.55 Intake @30.3psia, vacuum from 20.0 to 24.0psi @08:33:36.56 SS.reconfigure forSampling @08:33:36.59 SS.seek 25.00ml @08:33:38.57 Vacuum too high -- waiting up to 40s @08:33:39.25 Intake @29.7psia, vacuum from 20.0 to 24.0psi @08:33:39.26 SS.reconfigure forSampling @08:33:39.29 SS.seek 25.00ml @08:33:41.26 Vacuum too high -- waiting up to 40s @08:33:42.15 Intake @29.6psia, vacuum from 20.0 to 24.0psi @08:33:42.16 SS.reconfigure forSampling @08:33:42.19 SS.seek 25.00ml @08:33:44.10 Vacuum too high -- waiting up to 40s @08:33:44.78 Intake @27.1psia, vacuum from 20.0 to 24.0psi @08:33:44.79 SS.reconfigure forSampling @08:33:44.82 SS.seek 25.00ml @08:33:53.81 Vacuum too high -- waiting up to 40s @08:33:55.96 Intake @31.0psia, vacuum from 20.0 to 24.0psi @08:33:56.57 SS.reconfigure forSampling @08:33:56.61 SS.seek 25.00ml @08:33:59.00 Vacuum too high -- waiting up to 40s @08:33:59.71 Intake @31.0psia, vacuum from 20.0 to 24.0psi @08:33:59.72 SS.reconfigure forSampling @08:33:59.76 SS.seek 25.00ml @08:34:01.91 Vacuum too high -- waiting up to 40s @08:34:02.82 Intake @33.4psia, vacuum from 20.0 to 24.0psi @08:34:02.83 SS.reconfigure forSampling @08:34:02.87 SS.seek 25.00ml @08:34:04.57 Vacuum too high -- waiting up to 40s @08:34:05.25 Intake @34.0psia, vacuum from 20.0 to 24.0psi @08:34:05.26 SS.reconfigure forSampling @08:34:05.29 SS.seek 25.00ml @08:34:06.85 Vacuum too high -- waiting up to 40s @08:34:07.32 Intake @33.8psia, vacuum from 20.0 to 24.0psi @08:34:07.33 SS.reconfigure forSampling @08:34:07.36 SS.seek 25.00ml @08:34:08.54 Vacuum too high -- waiting up to 40s @08:34:09.23 Intake @34.1psia, vacuum from 20.0 to 24.0psi @08:34:09.24 SS.reconfigure forSampling @08:34:09.27 SS.seek 25.00ml @08:34:10.75 Vacuum too high -- waiting up to 40s @08:34:11.24 Intake @33.1psia, vacuum from 20.0 to 24.0psi @08:34:11.25 SS.reconfigure forSampling @08:34:11.28 SS.seek 25.00ml @08:34:12.47 Vacuum too high -- waiting up to 40s @08:34:12.95 Intake @30.4psia, vacuum from 20.0 to 24.0psi @08:34:12.96 SS.reconfigure forSampling @08:34:12.99 SS.seek 25.00ml @08:34:14.63 Vacuum too high -- waiting up to 40s @08:34:15.11 Intake @28.1psia, vacuum from 20.0 to 24.0psi @08:34:15.12 SS.reconfigure forSampling @08:34:15.15 SS.seek 25.00ml @08:34:22.03 Vacuum too high -- waiting up to 40s @08:34:23.76 Intake @32.7psia, vacuum from 20.0 to 24.0psi @08:34:23.77 SS.reconfigure forSampling @08:34:23.81 SS.seek 25.00ml @08:34:25.32 Vacuum too high -- waiting up to 40s @08:34:26.01 Intake @31.9psia, vacuum from 20.0 to 24.0psi @08:34:26.02 SS.reconfigure forSampling @08:34:26.05 SS.seek 25.00ml @08:34:27.75 Vacuum too high -- waiting up to 40s @08:34:28.45 Intake @32.4psia, vacuum from 20.0 to 24.0psi @08:34:28.46 SS.reconfigure forSampling @08:34:28.49 SS.seek 25.00ml @08:34:30.00 Vacuum too high -- waiting up to 40s @08:34:30.69 Intake @32.2psia, vacuum from 20.0 to 24.0psi @08:34:30.70 SS.reconfigure forSampling @08:34:30.73 SS.seek 25.00ml @08:34:32.32 Vacuum too high -- waiting up to 40s @08:34:33.02 Intake @32.0psia, vacuum from 20.0 to 24.0psi @08:34:33.03 SS.reconfigure forSampling @08:34:33.06 SS.seek 25.00ml @08:34:34.60 Vacuum too high -- waiting up to 40s @08:34:35.30 Intake @31.5psia, vacuum from 20.0 to 24.0psi @08:34:35.31 SS.reconfigure forSampling @08:34:35.34 SS.seek 25.00ml @08:34:37.25 Vacuum too high -- waiting up to 40s @08:34:38.35 Intake @35.6psia, vacuum from 20.0 to 24.0psi @08:34:38.36 SS.reconfigure forSampling @08:34:38.39 SS.seek 25.00ml @08:34:39.60 Vacuum too high -- waiting up to 40s @08:34:40.07 Intake @35.0psia, vacuum from 20.0 to 24.0psi @08:34:40.64 SS.reconfigure forSampling @08:34:40.68 SS.seek 25.00ml @08:34:42.50 Vacuum too high -- waiting up to 40s @08:34:42.98 Intake @34.7psia, vacuum from 20.0 to 24.0psi @08:34:42.99 SS.reconfigure forSampling @08:34:43.03 SS.seek 25.00ml @08:34:44.10 Vacuum too high -- waiting up to 40s @08:34:44.36 Intake @30.8psia, vacuum from 20.0 to 24.0psi @08:34:44.37 SS.reconfigure forSampling @08:34:44.41 SS.seek 25.00ml @08:34:46.10 Vacuum too high -- waiting up to 40s @08:34:47.02 Intake @32.0psia, vacuum from 20.0 to 24.0psi @08:34:47.03 SS.reconfigure forSampling @08:34:47.06 SS.seek 25.00ml @08:34:48.47 Vacuum too high -- waiting up to 40s @08:34:49.16 Intake @32.6psia, vacuum from 20.0 to 24.0psi @08:34:49.17 SS.reconfigure forSampling @08:34:49.20 SS.seek 25.00ml @08:34:50.50 Vacuum too high -- waiting up to 40s @08:34:51.20 Intake @32.9psia, vacuum from 20.0 to 24.0psi @08:34:51.21 SS.reconfigure forSampling @08:34:51.25 SS.seek 25.00ml @08:34:52.60 Vacuum too high -- waiting up to 40s @08:34:53.30 Intake @31.4psia, vacuum from 20.0 to 24.0psi @08:34:53.31 SS.reconfigure forSampling @08:34:53.34 SS.seek 25.00ml @08:34:55.13 Vacuum too high -- waiting up to 40s @08:34:56.05 Intake @32.6psia, vacuum from 20.0 to 24.0psi @08:34:56.06 SS.reconfigure forSampling @08:34:56.09 SS.seek 25.00ml @08:34:57.53 Vacuum too high -- waiting up to 40s @08:34:58.20 Intake @31.4psia, vacuum from 20.0 to 24.0psi @08:34:58.21 SS.reconfigure forSampling @08:34:58.25 SS.seek 25.00ml @08:34:59.91 Vacuum too high -- waiting up to 40s @08:35:00.81 Intake @32.0psia, vacuum from 20.0 to 24.0psi @08:35:00.82 SS.reconfigure forSampling @08:35:00.85 SS.seek 25.00ml @08:35:02.25 Vacuum too high -- waiting up to 40s @08:35:02.72 Intake @30.3psia, vacuum from 20.0 to 24.0psi @08:35:02.73 SS.reconfigure forSampling @08:35:02.76 SS.seek 25.00ml @08:35:04.31 Vacuum too high -- waiting up to 40s @08:35:05.21 Intake @28.9psia, vacuum from 20.0 to 24.0psi @08:35:05.22 SS.reconfigure forSampling @08:35:05.25 SS.seek 25.00ml @08:35:07.97 Vacuum too high -- waiting up to 40s @08:35:09.48 Intake @30.6psia, vacuum from 20.0 to 24.0psi @08:35:09.49 SS.reconfigure forSampling @08:35:09.53 SS.seek 25.00ml @08:35:11.00 Vacuum too high -- waiting up to 40s @08:35:11.90 Intake @30.7psia, vacuum from 20.0 to 24.0psi @08:35:11.91 SS.reconfigure forSampling @08:35:11.95 SS.seek 25.00ml @08:35:13.47 Vacuum too high -- waiting up to 40s @08:35:14.66 Intake @30.3psia, vacuum from 20.0 to 24.0psi @08:35:14.67 SS.reconfigure forSampling @08:35:14.71 SS.seek 25.00ml @08:35:16.47 Vacuum too high -- waiting up to 40s @08:35:16.72 Intake @26.5psia, vacuum from 20.0 to 24.0psi @08:35:16.73 SS.reconfigure forSampling @08:35:16.77 SS.seek 25.00ml @08:35:26.15 Vacuum too high -- waiting up to 40s @08:35:30.25 Intake @31.2psia, vacuum from 20.0 to 24.0psi @08:35:30.26 SS.reconfigure forSampling @08:35:30.29 SS.seek 25.00ml @08:35:32.38 Vacuum too high -- waiting up to 40s @08:35:33.51 Intake @32.2psia, vacuum from 20.0 to 24.0psi @08:35:33.52 SS.reconfigure forSampling @08:35:33.55 SS.seek 25.00ml @08:35:35.09 Vacuum too high -- waiting up to 40s @08:35:35.78 Intake @29.6psia, vacuum from 20.0 to 24.0psi @08:35:35.79 SS.reconfigure forSampling @08:35:35.82 SS.seek 25.00ml @08:35:38.99 Vacuum too high -- waiting up to 40s @08:35:40.52 Intake @29.9psia, vacuum from 20.0 to 24.0psi @08:35:40.53 SS.reconfigure forSampling @08:35:40.56 SS.seek 25.00ml @08:35:42.50 Vacuum too high -- waiting up to 40s @08:35:42.77 Intake @23.9psia, vacuum from 17.4 to 21.4psi @08:35:42.78 SS.reconfigure forSampling @08:35:42.81 SS.seek 25.00ml @08:35:48.37 Vacuum too high -- waiting up to 40s @08:35:54.32 Intake @31.3psia, vacuum from 20.0 to 24.0psi @08:35:54.33 SS.reconfigure forSampling @08:35:54.37 SS.seek 25.00ml @08:35:56.41 Vacuum too high -- waiting up to 40s @08:35:57.09 Intake @28.7psia, vacuum from 20.0 to 24.0psi @08:35:57.10 SS.reconfigure forSampling @08:35:57.13 SS.seek 25.00ml @08:36:00.27 Vacuum too high -- waiting up to 40s @08:36:02.63 Intake @29.9psia, vacuum from 20.0 to 24.0psi @08:36:02.64 SS.reconfigure forSampling @08:36:02.68 SS.seek 25.00ml @08:36:04.78 Vacuum too high -- waiting up to 40s @08:36:06.34 Intake @27.0psia, vacuum from 20.0 to 24.0psi @08:36:06.35 SS.reconfigure forSampling @08:36:06.39 SS.seek 25.00ml @08:36:12.52 Vacuum too high -- waiting up to 40s @08:36:16.22 Intake @28.0psia, vacuum from 20.0 to 24.0psi @08:36:16.23 SS.reconfigure forSampling @08:36:16.27 SS.seek 25.00ml @08:36:19.55 Vacuum too high -- waiting up to 40s @08:36:19.82 Intake @23.9psia, vacuum from 17.4 to 21.4psi @08:36:19.83 SS.reconfigure forSampling @08:36:19.86 SS.seek 25.00ml @08:36:23.88 Stroke completed -- waiting for pressure to equalize ... @08:36:31.23 SS.reconfigure SSconfig @08:36:31.27 SS.hold @08:36:31.36 Intake.select :close @08:36:31.49 ShallowSampler::Clogged in MAIN -- progress too slow after 400.0ml @08:36:31.50 SSV.dial :exhaust @08:36:32.30 Exhaust.select :open @08:36:32.40 SS.reconfigure SSexhale @08:36:32.44 SS.seek 0.00ml @08:36:41.57 Exhaust.select :close @08:36:41.67 Sampled 400.0ml @08:36:41.68 collectSample: Complete Evacuating Puck (bubblePt=55.0psi, delay=15.0s) @08:36:41.69 Intake.select :close @08:36:41.75 CSV.dial :waste @08:36:41.78 CS.seek 0.000ml @08:36:41.88 SSV.dial CBV,avoiding => exhaust @08:36:41.90
CBV.dial SSV @08:36:41.94 CSV.dial :air @08:36:42.81 CS.seek 10.000ml @08:36:43.95
CTV.dial :air @08:36:44.66 Sleepy.power :sampler,:OFF @08:36:44.75
delay 15 seconds @08:36:59.75 SSV.dial :spit,avoiding => exhaust @08:37:01.09 CTV.dial CSV,avoiding => intake @08:37:01.11
SS.seek 0.00ml @08:37:01.20 SSV.dial CBV,avoiding => exhaust @08:37:07.73 CBV.dial between CSV and waste2b @08:37:08.67 CTV.dial CSV,avoiding => air @08:37:08.69
CSV.dial CTV @08:37:09.14 CS.reconfigure CSpressurize @08:37:09.17 CS.seek 0.000ml @08:37:35.05 CSV.dial :air @08:37:35.58 CS.reconfigure CSfast @08:37:35.61 CS.seek 10.000ml @08:37:59.57 CSV.dial between air and CTV @08:37:59.95 CS.seek 2.217ml @08:38:18.69 CTV.dial CSV,avoiding => air @08:38:18.70
CSV.dial CTV @08:38:19.00 CS.reconfigure CSpressurize @08:38:19.03 CS.seek 0.000ml @08:38:20.51 BubblePoint: Intake @70.1psia, Outlet @14.9psia @08:38:20.52 SS.reconfigure SSconfig @08:38:20.55 SS.seek 10.00ml @08:38:26.61 delay 15 seconds @08:38:41.71 Evacuated: Intake @69.4psia, Outlet @-0.0psia SSV.dial :spit,avoiding => exhaust @08:38:43.04 SS.reconfigure SSexhale @08:38:43.07 SS.seek 0.00ml @08:38:48.48 SS.reconfigure SSfast @08:38:48.51 SS.seek 5.00ml @08:38:51.51 SSV.dial CBV,avoiding => exhaust @08:38:51.53
CBV.dial SSV @08:38:52.87 CTV.dial :air,avoiding => intake @08:38:54.16 CSV.dial CTV @08:38:54.18
SS.reconfigure SSconfig @08:38:54.22 SS.seek 10.00ml @08:38:55.18 CSV.dial :air @08:38:55.70 CS.reconfigure CSfast @08:38:55.73 CS.seek 0.000ml @08:38:57.80
SSV.dial :spit,avoiding => exhaust @08:38:59.12 SS.reconfigure SSexhale @08:38:59.15 SS.seek 0.00ml @08:39:00.77 CS.coast @08:39:04.20
SS.reconfigure SSfast @08:39:04.24 SS.seek 5.00ml @08:39:07.31 SSV.dial CBV,avoiding => exhaust @08:39:07.33
CBV.dial SSV @08:39:09.64 SSV.dial :spit,avoiding => exhaust @08:39:10.96 SS.reconfigure SSconfig @08:39:10.99 SS.seek 0.00ml @08:39:14.28 SS.coast @08:39:14.31 Evacuating Puck (bubblePt=55.0psi, delay=15.0s) Intake.select :close @08:39:14.37 CSV.dial :waste @08:39:14.46
SSV.dial :exhaust @08:39:15.12 CS.seek 0.000ml @08:39:15.22
Exhaust.select :open @08:39:15.32 SS.reconfigure SSexhale @08:39:15.36 SS.seek 0.00ml @08:39:15.55 CSV.dial :air @08:39:16.12
Exhaust.select :close @08:39:16.22 SSV.dial CBV,avoiding => exhaust @08:39:16.24
CBV.dial SSV @08:39:16.35 CS.seek 10.000ml @08:39:18.13
CTV.dial :air @08:39:18.14 delay 15 seconds @08:39:33.14 SSV.dial :spit,avoiding => exhaust @08:39:34.46 CTV.dial CSV,avoiding => intake @08:39:34.48
SS.seek 0.00ml @08:39:34.58 SSV.dial CBV,avoiding => exhaust @08:39:40.35 CBV.dial between CSV and waste2b @08:39:41.30 CSV.dial between air and CTV @08:39:41.60 CS.seek 7.582ml @08:39:48.02 CTV.dial CSV,avoiding => air @08:39:48.03
CSV.dial CTV @08:39:48.32 CS.reconfigure CSpressurize @08:39:48.35 CS.seek 0.000ml @08:40:08.11 CSV.dial :air @08:40:08.63 CS.reconfigure CSfast @08:40:08.66 CS.seek 10.000ml @08:40:32.55 CSV.dial between air and CTV @08:40:32.94 CS.seek 2.822ml @08:40:50.08 CTV.dial CSV,avoiding => air @08:40:50.09
CSV.dial CTV @08:40:50.38 CS.reconfigure CSpressurize @08:40:50.41 CS.seek 0.000ml @08:40:56.56 BubblePoint: Intake @72.7psia, Outlet @18.8psia @08:40:56.57 SS.reconfigure SSconfig @08:40:56.60 SS.seek 10.00ml @08:41:02.87 delay 15 seconds @08:41:17.97 Evacuated: Intake @71.7psia, Outlet @-0.0psia @08:41:17.98 SSV.dial :spit,avoiding => exhaust @08:41:19.30 SS.reconfigure SSexhale @08:41:19.33 SS.seek 0.00ml @08:41:24.62 SS.reconfigure SSfast @08:41:24.65 SS.seek 5.00ml @08:41:27.99 SSV.dial CBV,avoiding => exhaust @08:41:28.00
CBV.dial SSV @08:41:29.35 CTV.dial :air,avoiding => intake @08:41:30.65 CSV.dial CTV @08:41:30.67
SS.reconfigure SSconfig @08:41:30.71 SS.seek 10.00ml @08:41:31.67 CSV.dial :air @08:41:32.19 CS.reconfigure CSfast @08:41:32.22 CS.seek 0.000ml @08:41:33.85
SSV.dial :spit,avoiding => exhaust @08:41:34.45 CS.coast @08:41:35.16
SS.reconfigure SSexhale @08:41:35.19 SS.seek 0.00ml @08:41:40.51 SS.reconfigure SSfast @08:41:40.54 SS.seek 5.00ml @08:41:43.67 SSV.dial CBV,avoiding => exhaust @08:41:43.69
CBV.dial SSV @08:41:46.01 SSV.dial :spit,avoiding => exhaust @08:41:47.33 SS.reconfigure SSconfig @08:41:47.36 SS.seek 0.00ml @08:41:50.66 SS.coast @08:41:50.69 Lysing: Adding 1.5ml lysis CBV.dial between CSV and waste1,avoiding => DBV @08:41:52.18 CTV.dial CSV @08:41:53.45 CSV.dial CSR @08:41:54.24 CSR.select :lysis @08:41:54.34 CS.reconfigure CSconfig @08:41:54.38 CS.seek 1.000ml @08:41:57.82 delay 5 seconds @08:42:02.83 CSR.select :air @08:42:02.97 CS.seek 6.000ml @08:42:16.38 delay 2 seconds @08:42:18.39 CSV.dial :waste1 @08:42:19.66 CS.reconfigure CSfast @08:42:19.69 CS.seek empty @08:42:34.21 CSV.dial CSR @08:42:35.47 CSR.select :lysis @08:42:35.57 CS.reconfigure CSconfig @08:42:35.61 CS.seek 1.500ml @08:42:40.74 delay 5 seconds @08:42:45.76 CSR.select :air @08:42:45.90 CS.seek 4.501ml @08:42:54.30 delay 2 seconds @08:42:56.32 CSV.dial CTV @08:42:56.82 CS.seek 1.275ml @08:43:05.81 Heating sh1 puck from tube 3 in Collection Clamp to 85.0C for 5:00 @08:43:05.82 CH.seek 85.0C,8:00 @08:46:13.12 CH.hold 5:00,85.0C @08:51:13.85 Collection Puck Heater at 84.9C after lysing @08:51:13.87 CS.seek 0.000ml @08:51:17.88 CTV.dial between spare and air,avoiding => intake @08:51:18.87 CSV.dial :air @08:51:19.32 CS.seek 5.000ml @08:51:32.84 CSV.dial CTV @08:51:33.35 delay 2 seconds @08:51:35.36 CSV.dial :air @08:51:35.87 delay 2 seconds @08:51:37.88 CSV.dial CTV @08:51:38.40 delay 2 seconds @08:51:40.41 CSV.dial :air @08:51:40.93 delay 2 seconds @08:51:42.94 CS.seek 0.000ml @08:51:56.46 Recovering Lysate into Collection syringe @08:51:56.47 CSV.dial CBV @08:51:58.88 CBV.dial CSV @08:51:59.23 CS.seek 4.999ml @08:52:12.74 delay 5 seconds @08:52:17.76 CTV.dial :air @08:52:18.23 CS.seek 6.999ml @08:52:24.12 delay 5 seconds @08:52:29.14 CSV.dial :air2 @08:52:29.64 CS.seek 10.000ml @08:52:37.99 CSV.dial between air and CTV,avoiding => CBV @08:52:39.13 Lysing Complete @08:52:39.18 Storing sh1 puck in Collection Clamp to tube 2 @08:52:39.31 SC.reconfigure SCtight @08:52:39.34 SC.seek 2 @08:52:41.68 SC.reconfigure SCloose @08:52:41.84 Elbow.reconfigure TightElbow @08:52:41.88 Elbow.seek 2 @08:52:43.48 Forearm.reconfigure TightForearm @08:52:43.51 Forearm.seek 2 + 800 counts @08:52:44.46 SE.reconfigure SEpick @08:52:44.51 SE.seek beamBreak @08:52:46.17 SE.configure SEconfig @08:52:46.29 Elbow.reconfigure LimpElbow @08:52:46.32 Forearm.reconfigure LooseForearm @08:52:46.36 Forearm.seek 2 @08:52:46.61 Elbow.hold @08:52:46.66 Elbow.reconfigure TightElbow @08:52:46.74 SE.seek 13.03 Pucks below Top Plate @08:52:47.10 Forearm.coast @08:52:47.13 Forearm.seek 2 + 314 counts @08:52:47.37 Forearm.reconfigure TightForearm @08:52:47.40 Forearm.seek 2 + 800 counts @08:52:47.70 SE.seek 14.03 Pucks below Top Plate @08:52:48.42 Forearm.reconfigure LooseForearm @08:52:48.45 Forearm.seek retract @08:52:49.39 Elbow.seek CC @08:52:50.97 Elbow.reconfigure LooseElbow @08:52:51.06 Forearm.seek CC @08:52:52.01 CC.seek sealed @08:52:52.96 Hand.grip @08:52:53.73 CC.seek open @08:52:57.09 Forearm.seek retract @08:52:58.07 Elbow.reconfigure TightElbow @08:52:58.11 Elbow.seek 2 @08:52:59.70 Elbow.reconfigure LimpElbow @08:52:59.74 Forearm.seek 2 @08:53:00.74 Elbow.hold @08:53:00.79 Elbow.reconfigure TightElbow @08:53:00.82 Hand.release @08:53:01.69 Forearm.coast @08:53:01.72 Forearm.seek 2 + 300 counts @08:53:01.96 Forearm.reconfigure TightForearm @08:53:01.99 Forearm.seek 2 + 800 counts @08:53:02.19 SE.seek down @08:53:03.30
Forearm.reconfigure LooseForearm @08:53:03.34 Forearm.seek retract @08:53:03.52 SE.coast @08:53:04.25
SC.coast @08:53:04.28 Tranferring lysate to PRV @08:53:04.29 CSV.dial :air @08:53:04.58 CS.seek 10.000ml @08:53:05.18 CSV.dial between air and CTV @08:53:06.80 AIV.dial :bypass @08:53:06.87 Beginning samplePassC @08:53:06.88 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:53:07.02 Elbow.seek FlushPuck::Garage @08:53:08.71 Elbow.reconfigure LooseElbow @08:53:08.79 Forearm.seek garage @08:53:09.80 Hand.grip @08:53:10.57 Forearm.seek retract @08:53:11.60 Elbow.reconfigure TightElbow @08:53:11.64 Elbow.seek CC @08:53:14.38 Elbow.reconfigure LooseElbow @08:53:14.46 Forearm.reconfigure TightForearm @08:53:14.49 Forearm.seek CC @08:53:15.60 CC.seek closed @08:53:19.35 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 2.02 @08:53:19.40 Hand.release @08:53:20.15 Forearm.reconfigure LooseForearm @08:53:20.18 Forearm.seek retract @08:53:21.11 AWV.select :air @08:53:21.12 AIV.dial :bypass @08:53:21.13 PSV.dial PSR @08:53:22.54 PSR.select :flush @08:53:22.64 PS.seek 1.500ml @08:53:38.69 delay 5 seconds @08:53:43.71 PSV.dial :waste1 @08:53:44.14 PS.seek 0.000ml @08:54:00.19 PSV.dial PSR @08:54:00.63 PSR.select :air @08:54:00.73 PS.seek 2.500ml @08:54:27.05 CBV.dial PRV @08:54:27.06 PRV.dial CBV @08:54:27.83
PSV.dial :mixloop @08:54:28.96 CTV.dial :air,avoiding => intake @08:54:28.97 PTV.dial :PRVmixing @08:54:29.05 PS.seek 0.000ml @08:54:55.25 PSV.dial :air @08:54:55.72 PS.seek 2.500ml @08:55:21.97 PSV.dial :mixloop @08:55:22.42 PS.seek 0.000ml @08:55:48.72 PSV.dial :air @08:55:49.17 PS.seek 2.500ml @08:56:15.41 PSV.dial :mixloop @08:56:15.85 PS.seek 0.000ml @08:56:42.09 Garaging FlushPuck in Collection Clamp @08:56:42.15 Elbow.reconfigure TightElbow @08:56:42.18 Elbow.seek CC @08:56:42.27 Elbow.reconfigure LooseElbow @08:56:42.36 Forearm.seek CC @08:56:43.29 CC.seek sealed @08:56:44.20 Hand.grip @08:56:44.97 CC.seek open @08:56:48.26 Forearm.seek retract @08:56:49.23 Elbow.reconfigure TightElbow @08:56:49.27 Elbow.seek garage @08:56:52.08 Elbow.reconfigure LooseElbow @08:56:52.17 Forearm.reconfigure TightForearm @08:56:52.20 Forearm.seek garage @08:56:53.38 Hand.release @08:56:54.14 Forearm.reconfigure LooseForearm @08:56:54.17 Forearm.seek retract @08:56:55.21 Connecting PS to CS to transfer sample @08:56:55.22 CBV.dial AIV @08:56:55.23 AIV.dial :bypass @08:56:55.24 PSV.dial :mixloop @08:56:55.25 PTV.dial :PRVmixing PRV.dial AIV @08:56:55.26 CTV.dial :air,avoiding => intake @08:56:55.27 Loading lyfil puck from tube 3 into Collection Clamp @08:56:55.45 SC.reconfigure SCtight @08:56:55.48 SC.seek 3 @08:56:57.70 SC.reconfigure SCloose @08:56:57.87 Elbow.reconfigure TightElbow @08:56:57.90 Elbow.seek 3 @08:57:00.29 Forearm.reconfigure TightForearm @08:57:00.33 Forearm.seek 3 + 800 counts @08:57:01.40 SE.reconfigure SEpick @08:57:01.46 SE.seek beamBreak @08:57:03.82 SE.configure SEconfig @08:57:03.94 Elbow.seek 3 @08:57:04.08 Forearm.seek 3 + 300 counts @08:57:04.32 Forearm.reconfigure LooseForearm @08:57:04.36 Hand.grip @08:57:05.11 SE.seek down @08:57:05.25
Forearm.seek retract @08:57:06.22 Elbow.seek CC @08:57:07.25 SE.coast @08:57:07.81
Elbow.reconfigure LooseElbow @08:57:07.89 Forearm.reconfigure TightForearm @08:57:07.92 Forearm.seek CC @08:57:09.03 CC.seek closed @08:57:12.85 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.97 @08:57:12.86 Hand.release @08:57:13.64 Forearm.reconfigure LooseForearm @08:57:13.67 Forearm.seek retract @08:57:14.63 SC.coast @08:57:14.66 CTV.dial CSV,avoiding => intake @08:57:15.93 CSV.dial CTV @08:57:16.22 CS.seek 5.000ml @08:57:29.67 delay 1:20 @08:58:49.68 CS.seek 2.500ml @08:58:56.79 delay 40 seconds @08:59:36.81 CS.seek 0.000ml @08:59:44.01 delay 1:00 @09:00:44.02 PSV.dial :air @09:00:44.46 PS.seek 2.500ml @09:01:10.75 PSV.dial :mixloop @09:01:11.19 delay 3 seconds @09:01:14.20 PSV.dial :air @09:01:14.64 delay 2 seconds @09:01:16.65 PSV.dial :mixloop @09:01:17.09 delay 3 seconds @09:01:20.10 PSV.dial :waste1 @09:01:21.38 PS.seek empty @09:01:47.65 CBV.dial :waste1 @09:01:48.12 delay 5 seconds @09:01:53.13 CBV.dial AIV @09:01:53.59 delay 10 seconds @09:02:03.60 CBV.dial :waste1 @09:02:04.07 delay 10 seconds @09:02:14.08 CBV.dial AIV @09:02:14.54 delay 10 seconds @09:02:24.55 CTV.dial CSV,avoiding => intake @09:02:24.56 CBV.dial between AIV and waste1 @09:02:24.88 CSV.dial :air @09:02:25.41 CS.seek 10.000ml @09:02:51.60 CSV.dial CTV @09:02:52.12 CS.seek 0.000ml @09:03:18.22 CBV.dial :waste1 @09:03:18.52 delay 10 seconds @09:03:28.53 CTV.dial :air,avoiding => intake @09:03:29.87 Storing lyfil puck in Collection Clamp to tube 2 @09:03:30.00 SC.reconfigure SCtight @09:03:30.03 SC.seek 2 @09:03:32.23 SC.reconfigure SCloose @09:03:32.39 Elbow.reconfigure TightElbow @09:03:32.43 Elbow.seek 2 @09:03:34.06 Forearm.reconfigure TightForearm @09:03:34.09 Forearm.seek 2 + 800 counts @09:03:35.21 SE.reconfigure SEpick @09:03:35.26 SE.seek beamBreak @09:03:36.81 SE.configure SEconfig @09:03:36.93 Elbow.reconfigure LimpElbow @09:03:36.96 Forearm.reconfigure LooseForearm @09:03:37.00 Forearm.seek 2 @09:03:37.22 Elbow.hold @09:03:37.27 Elbow.reconfigure TightElbow @09:03:37.35 SE.seek 14.03 Pucks below Top Plate @09:03:37.68 Forearm.coast @09:03:37.71 Forearm.seek 2 + 341 counts @09:03:37.99 Forearm.reconfigure TightForearm @09:03:38.02 Forearm.seek 2 + 800 counts @09:03:38.31 SE.seek 15.03 Pucks below Top Plate @09:03:38.86 Forearm.reconfigure LooseForearm @09:03:38.90 Forearm.seek retract @09:03:39.86 Elbow.seek CC @09:03:42.04 Elbow.reconfigure LooseElbow @09:03:42.13 Forearm.seek CC @09:03:43.10 CC.seek sealed @09:03:44.00 Hand.grip @09:03:44.71 CC.seek open @09:03:48.08 Forearm.seek retract @09:03:49.03 Elbow.reconfigure TightElbow @09:03:49.06 Elbow.seek 2 @09:03:50.64 Elbow.reconfigure LimpElbow @09:03:50.69 Forearm.seek 2 @09:03:51.67 Elbow.hold @09:03:51.72 Elbow.reconfigure TightElbow @09:03:51.76 Hand.release @09:03:52.54 Forearm.coast @09:03:52.57 Forearm.seek 2 + 345 counts @09:03:52.85 Forearm.reconfigure TightForearm @09:03:52.88 Forearm.seek 2 + 800 counts @09:03:53.08 SE.seek down @09:03:54.19
Forearm.reconfigure LooseForearm @09:03:54.22 Forearm.seek retract @09:03:54.30 SE.coast @09:03:55.13
SC.coast @09:03:55.16 PSV.dial :air @09:03:56.21 PS.seek 0.499ml @09:04:02.18 delay 3 seconds @09:04:05.19 PSV.dial :mixloop @09:04:05.63 PRV.dial :air @09:04:06.07 delay 3 seconds @09:04:09.08 PRV.dial CBV @09:04:09.52 CBV.dial AIV @09:04:09.97 PRV.dial between CBV and air @09:04:10.27 PRV.dial :air @09:04:10.62 PS.reconfigure PSslow @09:04:10.65 PS.seek 0.599ml @09:04:13.15 PRV.dial :waste2 @09:04:13.88 PS.reconfigure PSconfig @09:04:13.91 PS.seek empty @09:04:20.84 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @09:04:20.98 Elbow.seek FlushPuck::Garage @09:04:22.76 Elbow.reconfigure LooseElbow @09:04:22.85 Forearm.seek garage @09:04:23.87 Hand.grip @09:04:24.67 Forearm.seek retract @09:04:25.67 Elbow.reconfigure TightElbow @09:04:25.71 Elbow.seek CC @09:04:28.54 Elbow.reconfigure LooseElbow @09:04:28.63 Forearm.reconfigure TightForearm @09:04:28.66 Forearm.seek CC @09:04:29.75 CC.seek closed @09:04:33.56 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 2.03 @09:04:33.61 Hand.release @09:04:34.38 Forearm.reconfigure LooseForearm @09:04:34.41 Forearm.seek retract @09:04:35.42 Clearing PML for modified lysate CTV.dial :air,avoiding => intake @09:04:35.43 PRV.dial :waste2 @09:04:35.44 PTV.dial :PRV,avoiding => puck @09:04:36.17 delay 2 seconds @09:04:38.17 PTV.dial :PRVmixing,avoiding => puck @09:04:38.90 delay 2 seconds @09:04:40.91 PSV.dial :PML @09:04:40.92 delay 2 seconds @09:04:42.92 PRV.dial :air @09:04:43.63 delay 2 seconds @09:04:45.69 PS.seek 1.499ml @09:05:01.80 delay 5 seconds @09:05:06.82 PS.seek 2.500ml @09:05:17.86 PSV.dial :air @09:05:18.33 PS.seek 0.000ml @09:05:44.64 mixing lysate with speDil @09:05:44.65 PSV.dial :PML @09:05:45.10 CBV.dial :PRV @09:05:45.11 PRV.dial :air @09:05:45.70 delay 2 seconds @09:05:47.71 PRV.dial :speDil @09:05:48.16 delay 2 seconds @09:05:50.22 PS.reconfigure PSslow1 @09:05:50.25 PS.seek 0.225ml @09:06:13.61 delay 5 seconds @09:06:18.62 PRV.dial CBV @09:06:19.35 delay 2 seconds @09:06:21.41 PS.seek 0.475ml @09:06:47.14 delay 5 seconds @09:06:52.15 PRV.dial :air @09:06:52.59 delay 2 seconds @09:06:54.60 PS.seek 0.925ml @09:07:40.86 delay 10 seconds @09:07:50.87 PRV.dial CBV @09:07:51.33 CTV.dial :air,avoiding => intake @09:07:51.34 PSV.dial :air @09:07:51.92 PS.reconfigure PSconfig @09:07:51.95 PS.seek 2.500ml @09:08:08.76 delay 3 seconds @09:08:11.77 PSV.dial PTV @09:08:12.59 PTV.dial :PRV,avoiding => puck @09:08:13.32 PS.seek 0.000ml @09:08:39.61 PSV.dial :air @09:08:40.32 PS.seek 2.500ml @09:09:06.51 delay 3 seconds @09:09:09.53 PSV.dial PTV @09:09:10.25 PTV.dial :PRV,avoiding => puck @09:09:10.27 PS.seek 0.000ml @09:09:36.48 Pushing modified lysate into AIV Injection Loop @09:09:36.49 CBV.dial AIV @09:09:36.50 AIV.dial :bypass @09:09:36.51 PRV.dial CBV @09:09:36.52 PSV.dial :air @09:09:37.24 PS.seek 2.500ml @09:10:03.42 delay 3 seconds @09:10:06.44 PSV.dial :PML @09:10:06.87 PTV.dial :PRVmixing,avoiding => puck @09:10:07.65 PS.reconfigure PSslow1 @09:10:07.68 PS.seek 1.850ml @09:11:14.13 delay 5 seconds @09:11:19.14 AIV.dial :sample @09:11:19.67 AWV.select :waste @09:11:19.82 PS.reconfigure PSslow2 @09:11:19.85 PS.seek 1.310ml @09:13:09.97 delay 15 seconds @09:13:24.98 AIV.dial :bypass @09:13:25.52 PS.reconfigure PSconfig @09:13:25.55 PS.seek 0.000ml @09:13:39.66 PSV.dial :air @09:13:40.11 PS.seek 2.500ml @09:14:06.38 PSV.dial :PML @09:14:06.82 PS.seek 0.000ml @09:14:33.16 AIV.dial :coilToMFB @09:14:33.17 AWV.select :air @09:14:33.31 Extracting Nucleic Acid from AIV @09:14:33.33
Beginning samplePassD @09:14:33.34 SPE.hold 2:46:40,55.0C @09:14:33.36 ASV.dial :SPEdelivery @09:14:33.49
AWV.select :air @09:14:33.50 AIV.dial :bypass @09:14:33.51 PSV.dial PSR @09:14:33.75 delay 20 seconds @09:14:34.53
PSR.select :killMFB @09:14:34.63 PS.seek 1.500ml @09:14:50.69 delay 5 seconds @09:14:53.76 primeLysateINT @09:14:53.77 AWV.select :air @09:14:53.78 AIV.dial :coilToMFB @09:14:53.79 ATV.dial :AIV @09:14:55.14 AS.reconfigure ASslow1 @09:14:55.18 AS.seek 0.239ml @09:14:55.70
PSV.dial :waste1 @09:14:56.15 PS.seek 0.000ml @09:15:12.22 PSV.dial PSR @09:15:12.67 PSR.select :air @09:15:12.77 PS.seek 2.500ml @09:15:39.12 PRV.dial CBV @09:15:39.14
CBV.dial PRV @09:15:39.15 PSV.dial :mixloop @09:15:40.18 CTV.dial :air,avoiding => intake @09:15:40.19 PTV.dial :PRVmixing @09:15:40.21 PS.seek 0.000ml @09:15:44.43 delay 10 seconds @09:15:54.44 ATV.dial :air2 @09:15:55.03 AS.seek 0.269ml @09:16:01.40 ATV.dial :waste2 @09:16:01.87 AS.reconfigure ASnorm @09:16:01.90 AS.seek empty @09:16:06.50
PSV.dial PSR @09:16:07.54 PSR.select :flush @09:16:07.64 PS.seek 1.500ml @09:16:16.07 Lysate primed! @09:16:16.08 AFV.select :holdingCoil @09:16:16.09 ATV.dial :air2 @09:16:16.58 AS.reconfigure ASslow1 @09:16:16.61 AS.seek 0.069ml @09:16:23.81
delay 5 seconds @09:16:28.83 PSV.dial :waste1 @09:16:29.28 PS.seek 0.000ml @09:16:31.13 delay 3 seconds @09:16:34.14 ATV.dial :AIV @09:16:34.73 AS.seek 0.469ml @09:16:45.41
PSV.dial PSR @09:16:45.86 PSR.select :air @09:16:45.96 PS.seek 2.500ml @09:17:12.16 PRV.dial CBV @09:17:12.18
CBV.dial PRV @09:17:12.67 PSV.dial :mixloop @09:17:13.71 CTV.dial :air,avoiding => intake @09:17:13.72 PTV.dial :PRVmixing @09:17:13.74 PS.seek 0.000ml @09:17:40.00 PSV.dial PSR @09:17:41.03 PSR.select :flush @09:17:41.13 PS.seek 1.500ml @09:17:56.58 delay 5 seconds @09:17:57.22
delay 5 seconds @09:18:01.63 Solid Phase Extraction Heater at 52.0C @09:18:01.64 ATV.dial ASV @09:18:01.97 ASV.dial :SPEdelivery @09:18:02.03 AS.seek 0.041ml @09:18:02.23
PSV.dial :waste1 @09:18:02.68 PS.seek 0.000ml @09:18:18.81 PSV.dial PSR @09:18:19.27 PSR.select :air @09:18:19.37 PS.seek 2.500ml @09:18:45.69 PRV.dial CBV @09:18:45.71
CBV.dial PRV @09:18:45.72 PSV.dial :mixloop @09:18:46.72 CTV.dial :air,avoiding => intake @09:18:46.73 PTV.dial :PRVmixing @09:18:46.75 PS.seek 0.000ml @09:19:13.19 PSV.dial PSR @09:19:14.20 PSR.select :flush @09:19:14.30 PS.seek 1.500ml @09:19:29.55 delay 10 seconds @09:19:30.44
delay 5 seconds @09:19:35.45 PSV.dial :waste1 @09:19:35.90 PS.seek 0.000ml @09:19:39.56 rinseLysateLineINT @09:19:39.57 AFV.select :carrierSolution @09:19:39.66 delay 2 seconds @09:19:41.67 AS.reconfigure ASconfig @09:19:41.70 AS.seek 1.000ml @09:19:51.97
PSV.dial PSR @09:19:52.42 PSR.select :air @09:19:52.52 PS.seek 2.500ml @09:20:18.84 PRV.dial CBV @09:20:18.86
CBV.dial PRV @09:20:18.87 PSV.dial :mixloop @09:20:19.87 CTV.dial :air,avoiding => intake @09:20:19.88 PTV.dial :PRVmixing @09:20:19.90 PS.reconfigure PSslow @09:20:19.93 PS.seek 1.200ml @09:20:37.86 delay 1 second @09:20:38.87 AFV.select :holdingCoil @09:20:38.96 delay 1 second @09:20:39.97 ATV.dial :AIV @09:20:40.31 AIV.dial :coilToMFB @09:20:40.32 AWV.select :waste @09:20:40.42 AS.reconfigure ASslow0 @09:20:40.46 AS.seek 0.000ml @09:20:46.90
PSV.dial :waste1 @09:20:48.21 PS.reconfigure PSconfig @09:20:48.24 PS.seek 0.000ml @09:21:01.24 PSV.dial :air @09:21:02.27 PS.seek 2.500ml @09:21:28.46 PSV.dial :mixloop @09:21:28.91 PS.reconfigure PSslow @09:21:28.94 PS.seek 0.000ml @09:22:20.40 PSV.dial :air @09:22:20.97 PS.reconfigure PSconfig @09:22:21.00 PS.seek 2.500ml @09:22:22.81 ATV.dial :air2 @09:22:23.37 AS.reconfigure ASslow1 @09:22:23.40 AS.seek 0.050ml @09:22:33.87 ATV.dial :AIV @09:22:34.45 AS.seek 0.010ml @09:22:42.87 ATV.dial :waste @09:22:44.16 AS.seek empty @09:22:46.60 ethanol wash @09:22:46.61 ATV.dial :air2 @09:22:47.27
PSV.dial :mixloop @09:22:47.56 AS.reconfigure ASslow2 @09:22:47.59 AS.seek 0.009ml @09:22:47.70
PS.reconfigure PSslow @09:22:47.73 PS.seek 0.000ml @09:22:58.20 delay 3 seconds @09:23:01.21 Pulling 0.4ml of Ethanol Wash @09:23:01.22 ATV.dial :ethanol @09:23:01.79 AS.reconfigure ASslow1 @09:23:01.82 AS.seek 0.409ml @09:23:39.24
Cleaning Collection Syringe @09:23:39.25 CSV.dial CSR @09:23:39.68 CSR.select :flush @09:23:39.78 CS.seek 4.000ml @09:23:50.77 delay 5 seconds @09:23:55.78 CSR.select :air @09:23:55.87 CS.seek 10.000ml @09:24:11.93 CSV.dial CTV @09:24:11.95
CTV.dial CSV @09:24:13.27 CBV.dial :waste1 @09:24:13.73 CS.seek 0.000ml @09:24:23.65 delay 3 seconds @09:24:26.66 ATV.dial :air1 @09:24:26.99 delay 3 seconds @09:24:30.05 AS.seek 0.434ml @09:24:35.41 delay 3 seconds @09:24:38.42 ATV.dial :ethanol @09:24:38.81 AS.seek 0.419ml @09:24:39.86
CSV.dial CSR @09:24:40.36 CSR.select :flush @09:24:40.46 CS.seek 4.000ml @09:24:42.13 delay 3 seconds @09:24:45.14 ATV.dial ASV @09:24:46.38 AS.seek 0.092ml @09:24:51.36
delay 5 seconds @09:24:56.37 CSR.select :air @09:24:56.97 CS.seek 10.000ml @09:25:12.92 CSV.dial CTV @09:25:12.94
CTV.dial CSV @09:25:13.45 CBV.dial :waste1 @09:25:13.46 CS.seek 0.000ml @09:25:39.69 Garaging FlushPuck in Collection Clamp @09:25:39.74 Elbow.reconfigure TightElbow @09:25:39.78 Elbow.seek CC @09:25:39.88 Elbow.reconfigure LooseElbow @09:25:39.96 Forearm.seek CC @09:25:40.93 CC.seek sealed @09:25:41.86 Hand.grip @09:25:42.54 CC.seek open @09:25:45.82 Forearm.seek retract @09:25:46.77 Elbow.reconfigure TightElbow @09:25:46.81 Elbow.seek garage @09:25:49.60 Elbow.reconfigure LooseElbow @09:25:49.68 Forearm.reconfigure TightForearm @09:25:49.71 Forearm.seek garage @09:25:50.86 Hand.release @09:25:51.58 Forearm.reconfigure LooseForearm @09:25:51.61 Forearm.seek retract @09:25:52.62 Whole Cell Archive sampling 1000ml @09:25:52.64 Loading wcr puck from tube 3 into Collection Clamp @09:25:52.82 SC.reconfigure SCtight @09:25:52.86 SC.seek 3 @09:25:53.33 delay 3 seconds @09:25:55.11 SC.reconfigure SCloose @09:25:55.29 Elbow.reconfigure TightElbow @09:25:55.32 Elbow.seek 3 @09:25:56.34 ATV.dial :waste2 @09:25:56.87 AS.reconfigure ASconfig @09:25:56.90 AS.seek empty @09:25:57.19 Forearm.reconfigure TightForearm @09:25:57.22 Forearm.seek 3 + 800 counts @09:25:58.21 SE.reconfigure SEpick @09:25:58.27 SE.seek beamBreak @09:26:00.73 SE.configure SEconfig @09:26:00.86 Elbow.seek 3 @09:26:01.04 Forearm.seek 3 + 300 counts @09:26:01.36 Forearm.reconfigure LooseForearm @09:26:01.40 Hand.grip @09:26:02.18 SE.seek down @09:26:02.32 Forearm.seek retract @09:26:02.63 ATV.dial :air2 @09:26:02.97 AS.reconfigure ASslow1 @09:26:03.00 AS.seek 1.000ml @09:26:03.29 Elbow.seek CC @09:26:04.44 SE.coast @09:26:04.85 Elbow.reconfigure LooseElbow @09:26:04.93 Forearm.reconfigure TightForearm @09:26:04.96 Forearm.seek CC @09:26:06.08 CC.seek closed @09:26:09.85 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.92 @09:26:09.86 Hand.release @09:26:10.58 Forearm.reconfigure LooseForearm @09:26:10.61 Forearm.seek retract @09:26:11.61 SC.coast @09:26:11.64 collectSample 1000ml w/bubble point of 55psi @09:26:11.66 Priming 7ml Sleepy.power :sampler,:ON @09:26:11.67 Intake.select :close @09:26:11.68 Exhaust.select :close @09:26:11.69 SS.hold @09:26:11.84 Calibrating pressure sensors @09:26:11.85 CTV.dial :air @09:26:13.17 SSV.dial :spit,avoiding => exhaust @09:26:13.18 SS.reconfigure SSfast @09:26:13.21 SS.seek 5.00ml @09:26:15.87 SSV.dial CBV @09:26:16.46 CBV.dial SSV @09:26:17.88 delay 3.8 seconds @09:26:21.77 SSV.dial :spit @09:26:23.14 SS.seek 0.00ml @09:26:25.91 SSV.dial CBV @09:26:27.27 CTV.dial :intake @09:26:28.01 Waiting for external sampler to finish priming... @09:26:28.02 delayUntil "09:36:11.66" @09:29:27.39 delay 5 seconds @09:29:32.40 ATV.dial ASV @09:29:33.15 ASV.dial :SPEdelivery @09:29:33.17 AS.seek 0.020ml @09:32:53.36 delay 5 seconds @09:32:58.37 ATV.dial :air2 @09:32:59.27 AS.seek 0.520ml @09:34:41.53 delay 5 seconds @09:34:46.54 ATV.dial ASV @09:34:47.29 ASV.dial :wasteR @09:34:47.91 AS.seek 0.300ml @09:35:33.06 delay 20 seconds @09:35:53.07 AS.seek 0.070ml @09:36:11.67 SSV.dial CBV @09:36:11.69 CBV.dial SSV @09:36:11.70 Allowing 55s for 7.0ml of remaining 7.0ml @09:36:11.71 Intake.select :open @09:36:13.84 Intake @15.1psia, vacuum from 8.6 to 12.6psi @09:36:13.86 SS.reconfigure forSampling @09:36:13.89 SS.seek 7.00ml @09:36:19.83 Stroke completed -- waiting for pressure to equalize ... @09:36:20.47 Intake.select :close @09:36:20.61 SSV.dial :exhaust @09:36:21.42 Exhaust.select :open @09:36:21.53 SS.reconfigure SSexhale @09:36:21.57 SS.seek 0.00ml @09:36:25.69 Exhaust.select :close @09:36:25.80 Sampling 1000.0ml @09:36:25.81 Intake.select :close @09:36:25.82 Exhaust.select :close @09:36:25.83 SS.reconfigure SSconfig @09:36:25.86 SS.hold @09:36:26.02 CTV.dial :intake @09:36:26.04 SSV.dial CBV @09:36:26.06 CBV.dial SSV @09:36:26.90 Allowing 185s for 25.0ml of remaining 1000.0ml @09:36:26.91 Intake.select :open @09:36:29.04 Intake @14.1psia, vacuum from 7.6 to 11.6psi @09:36:29.06 SS.reconfigure forSampling @09:36:29.09 SS.seek 25.00ml @09:36:33.66 Vacuum too high -- waiting up to 40s @09:36:40.21 delay 20 seconds @09:37:00.22 ATV.dial :waste2 @09:37:00.81 AS.reconfigure ASconfig @09:37:00.85 AS.seek empty @09:37:05.30 Elution @09:37:05.31 ATV.dial :air1 @09:37:07.12 AS.reconfigure ASslow1 @09:37:07.15 AS.seek 0.049ml @09:37:13.86 SS.reconfigure SSconfig @09:37:13.90 SS.hold @09:37:13.99 Intake.select :close @09:37:14.14 ShallowSampler::Clogged in WCR -- Intake @14.7psia, & Outlet @2.1psia -- no progress after 5.4ml @09:37:14.15 SSV.dial :exhaust @09:37:14.95 Exhaust.select :open @09:37:15.05 SS.reconfigure SSexhale @09:37:15.09 SS.seek 0.00ml @09:37:17.68 delay 3 seconds @09:37:18.64 Exhaust.select :close @09:37:18.75 Sampled 5.4ml @09:37:18.76 collectSample: Complete @09:37:18.77 Drying puck before fixing for 04:00.00 CBV.dial :waste2b @09:37:19.58 delay 1:00 @09:37:20.69 ATV.dial :ElutionWater @09:37:21.29 AS.seek 0.109ml @09:37:21.74 Sleepy.power :sampler,:OFF @09:37:33.77 delay 3 seconds @09:37:36.78 ATV.dial ASV,via => air2 @09:37:38.44 ASV.dial :SPEelution @09:37:38.94 AFV.select :carrierSolution @09:37:39.09 AS.reconfigure ASnorm @09:37:39.12 AS.seek 0.759ml @09:38:12.61 delay 3 seconds @09:38:15.62 AFV.select :holdingCoil @09:38:15.76 AS.reconfigure ASslow1 @09:38:15.79 AS.seek 0.489ml @09:38:19.58 CSV.dial :air @09:38:20.03 CS.reconfigure CSfast @09:38:20.06 CS.seek 10.000ml @09:38:43.96 CBV.dial between CSV and waste2b @09:38:44.28 Pumping wcr puck from tube 3 up to 55psi... @09:38:44.32 CBV.dial SSV @09:38:45.75 CBV.dial between CSV and waste2b @09:38:46.70 CSV.dial between air and CTV @09:38:47.09 CS.seek 7.027ml @09:38:54.66 CTV.dial CSV,avoiding => air @09:38:54.68 CSV.dial CTV @09:38:55.44 CS.reconfigure CSpressurize @09:38:55.47 CS.seek 0.000ml @09:39:11.11 delay 5 seconds @09:39:13.83 CSV.dial :air @09:39:14.36 CS.reconfigure CSfast @09:39:14.39 CS.seek 10.000ml @09:39:16.17 AS.reconfigure ASslow2 @09:39:16.20 AS.seek 0.454ml @09:39:38.22 CSV.dial between air and CTV @09:39:38.61 CS.seek 2.480ml @09:39:52.10 delay 1:00 @09:39:56.59 CTV.dial CSV,avoiding => air @09:39:56.60 CSV.dial CTV @09:39:56.89 CS.reconfigure CSpressurize @09:39:56.92 CS.seek 0.000ml @09:39:59.88 CBV.dial :waste2b @09:40:00.20 CS.reconfigure CSconfig @09:40:00.23 CS.seek empty @09:40:04.71 delay 1:00 @09:40:52.11 pushing eluate to holding coil @09:40:52.17 AS.seek 0.294ml @09:41:04.72 CTV.dial :air,avoiding => intake @09:41:06.00 Fix #1 of 2 (with rnal) for 02:00.00 @09:41:06.01 CS Pulling 2ml of rnal CSV.dial CSR @09:41:06.44 CSR.select :rnal @09:41:06.54 CS.seek 2.000ml @09:41:12.45 delay 5 seconds @09:41:17.46 CSR.select :air @09:41:17.55 CS.reconfigure CSfast @09:41:17.59 CS.seek 10.000ml @09:41:36.67 CBV.dial between CSV and waste2b @09:41:36.99 Pumping wcr puck from tube 3 up to 55psi... @09:41:37.04 CBV.dial SSV @09:41:38.48 CBV.dial between CSV and waste2b @09:41:39.42 CSV.dial between air and CTV @09:41:40.07 CS.seek 6.828ml @09:41:48.09 CTV.dial CSV,avoiding => air @09:41:48.11 CSV.dial CTV @09:41:49.46 CS.reconfigure CSpressurize @09:41:49.49 CS.seek 0.000ml @09:42:06.41 delay 2:00 @09:43:35.46 delay 30 seconds @09:44:05.47 ATV.dial :waste,via => air3 @09:44:06.42 CBV.dial :waste2b @09:44:06.74 CS.reconfigure CSconfig @09:44:06.77 CS.seek empty @09:44:06.93 AS.reconfigure ASconfig @09:44:06.96 AS.seek empty @09:44:08.32 delay 1:00 @09:44:24.45 Parking Bleach in LysateSource line and SPEcolumn @09:44:24.46 ATV.dial :air3 @09:44:25.03 AS.reconfigure ASslow1 @09:44:25.06 AS.seek 0.029ml @09:44:31.49 ATV.dial :ElutionWater @09:44:31.98 AS.seek 0.059ml @09:44:38.38 ATV.dial :air3 @09:44:38.76 AS.seek 0.069ml @09:44:41.07 delay 2 seconds @09:44:43.08 ATV.dial :bleach @09:44:43.56 AS.seek 0.709ml @09:45:08.33 CTV.dial :air,avoiding => intake @09:45:09.61 Fix #2 of 2 (with rnal) for 02:00.00 @09:45:09.62 CS Pulling 2ml of rnal CSV.dial CSR @09:45:10.15 CSR.select :rnal @09:45:10.25 CS.seek 2.000ml @09:45:16.22 delay 5 seconds @09:45:21.23 CSR.select :air @09:45:21.33 CS.reconfigure CSfast @09:45:21.37 CS.seek 10.000ml @09:45:40.57 CBV.dial between CSV and waste2b @09:45:40.90 Pumping wcr puck from tube 3 up to 55psi... @09:45:40.95 CBV.dial SSV @09:45:42.37 CBV.dial between CSV and waste2b @09:45:43.32 CSV.dial between air and CTV @09:45:44.00 CS.seek 6.780ml @09:45:52.26 CTV.dial CSV,avoiding => air @09:45:52.28 CSV.dial CTV @09:45:53.59 CS.reconfigure CSpressurize @09:45:53.62 CS.seek 0.000ml @09:46:10.40 delay 2:00 @09:46:54.38 delay 3 seconds @09:46:57.39 ATV.dial :air3 @09:46:57.72 delay 3 seconds @09:47:00.78 AS.seek 0.734ml @09:47:06.13 delay 3 seconds @09:47:09.14 ATV.dial :bleach @09:47:09.54 AS.seek 0.719ml @09:47:12.85 delay 3 seconds @09:47:15.86 ATV.dial :AIV @09:47:17.04 AS.reconfigure ASconfig @09:47:17.07 AS.seek 0.015ml @09:47:58.39 ATV.dial :waste @09:47:59.68 AS.seek empty @09:48:00.88 ATV.dial :air3 @09:48:01.44 AS.reconfigure ASslow1 @09:48:01.47 AS.seek 0.009ml @09:48:03.81 delay 2 seconds @09:48:05.82 ATV.dial :bleach @09:48:06.19 AS.seek 0.579ml @09:48:10.41 CBV.dial :waste2b @09:48:10.74 CS.reconfigure CSconfig @09:48:10.77 CS.seek empty @09:48:12.56 delay 1:00 @09:49:12.57 CTV.dial :air,avoiding => intake @09:49:13.89 Storing wcr puck in Collection Clamp to tube 2 @09:49:14.02 SC.reconfigure SCtight @09:49:14.05 SC.seek 2 @09:49:16.25 SC.reconfigure SCloose @09:49:16.97 Elbow.reconfigure TightElbow @09:49:17.00 Elbow.seek 2 @09:49:18.61 Forearm.reconfigure TightForearm @09:49:18.64 Forearm.seek 2 + 800 counts @09:49:19.67 SE.reconfigure SEpick @09:49:19.72 SE.seek beamBreak @09:49:21.21 SE.configure SEconfig @09:49:21.34 Elbow.reconfigure LimpElbow @09:49:21.38 Forearm.reconfigure LooseForearm @09:49:21.42 Forearm.seek 2 @09:49:21.63 Elbow.hold @09:49:21.69 Elbow.reconfigure TightElbow @09:49:21.76 SE.seek 15.03 Pucks below Top Plate @09:49:22.17 Forearm.coast @09:49:22.21 Forearm.seek 2 + 349 counts @09:49:22.50 Forearm.reconfigure TightForearm @09:49:22.54 Forearm.seek 2 + 800 counts @09:49:22.72 SE.seek 16.03 Pucks below Top Plate @09:49:23.27 Forearm.reconfigure LooseForearm @09:49:23.30 Forearm.seek retract @09:49:24.27 Elbow.seek CC @09:49:25.87 Elbow.reconfigure LooseElbow @09:49:25.95 Forearm.seek CC @09:49:26.91 CC.seek sealed @09:49:27.84 Hand.grip @09:49:28.61 CC.seek open @09:49:31.98 Forearm.seek retract @09:49:32.93 Elbow.reconfigure TightElbow @09:49:32.96 Elbow.seek 2 @09:49:34.58 Elbow.reconfigure LimpElbow @09:49:34.62 Forearm.seek 2 @09:49:35.57 Elbow.hold @09:49:35.62 Elbow.reconfigure TightElbow @09:49:35.66 Hand.release @09:49:36.44 Forearm.coast @09:49:36.48 Forearm.seek 2 + 327 counts @09:49:36.78 Forearm.reconfigure TightForearm @09:49:36.81 Forearm.seek 2 + 800 counts @09:49:37.03 SE.seek down @09:49:38.13 Forearm.reconfigure LooseForearm @09:49:38.17 Forearm.seek retract @09:49:38.31 SE.coast @09:49:39.09 SC.coast @09:49:39.12 Starting finalPurge @09:49:39.13 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @09:49:39.28 Elbow.seek FlushPuck::Garage @09:49:40.97 Elbow.reconfigure LooseElbow @09:49:41.05 Forearm.seek garage @09:49:42.07 Hand.grip @09:49:42.95 Forearm.seek retract @09:49:43.97 Elbow.reconfigure TightElbow @09:49:44.00 Elbow.seek CC @09:49:46.75 Elbow.reconfigure LooseElbow @09:49:46.83 Forearm.reconfigure TightForearm @09:49:46.86 Forearm.seek CC @09:49:48.59 CC.seek closed @09:49:52.40 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 2.01 @09:49:52.45 Hand.release @09:49:53.28 Forearm.reconfigure LooseForearm @09:49:53.31 Forearm.seek retract @09:49:54.29 Flushing the Collection Valve::Manifold @09:49:54.39 CS Pulling 5ml of flush @09:49:54.40 CSV.dial CSR @09:49:54.82 CSR.select :flush @09:49:54.93 CS.seek 5.000ml @09:50:02.70 delay 3 seconds @09:50:05.71 ATV.dial :air3 @09:50:06.04 delay 3 seconds @09:50:08.40
delay 5 seconds @09:50:09.10 AS.seek 0.604ml @09:50:13.41
CSR.select :air @09:50:13.55 CS.reconfigure CSfast @09:50:13.58 CS.seek 7.499ml @09:50:14.48 delay 3 seconds @09:50:17.49 ATV.dial :bleach @09:50:17.88 AS.seek 0.589ml @09:50:20.18
CSV.dial :waste @09:50:21.20 delay 3 seconds @09:50:21.45
CS.reconfigure CSconfig @09:50:21.48 CS.seek 0.000ml @09:50:24.21 ATV.dial :ASV @09:50:25.20 ASV.dial :wasteR @09:50:26.09 AS.seek 0.000ml @09:50:41.31
Flushing lower manifold's waste1 port @09:50:41.40 CSV.dial :flush @09:50:41.84 CS.reconfigure CSfast @09:50:41.87 CS.seek 5.000ml @09:50:54.22 delay 5 seconds @09:50:59.23 CSV.dial :air @09:50:59.72 CS.seek 7.000ml @09:51:05.12 CSV.dial CTV @09:51:05.14
CTV.dial CSV @09:51:06.48 CBV.dial :waste1 @09:51:07.23 CS.seek 0.000ml @09:51:23.81 Flushing lower manifold's waste2b port @09:51:23.90 CSV.dial :flush @09:51:24.71 CS.seek 5.000ml @09:51:36.97 delay 5 seconds @09:51:41.98 CSV.dial :air @09:51:42.75 CS.seek 7.000ml @09:51:48.15 CSV.dial CTV @09:51:48.17
CTV.dial CSV @09:51:48.70 CBV.dial :waste2b @09:51:49.44 CS.seek 0.000ml @09:52:06.43 Flushing upper manifold @09:52:06.52 CSV.dial :flush @09:52:07.33 CS.seek 5.000ml @09:52:19.56 delay 5 seconds @09:52:24.57 CSV.dial :air @09:52:25.34 CS.seek 7.000ml @09:52:26.57 delay 1:00 @09:52:30.84 CSV.dial CBV @09:52:30.86
CBV.dial CSV @09:52:31.90 CTV.dial :air @09:52:33.18 CS.seek 0.000ml @09:52:49.71 delay 7 seconds @09:52:56.72 CS.seek 3.000ml @09:53:04.40 CSV.dial :waste @09:53:04.91 CS.seek 0.000ml @09:53:12.64 CSV.dial :flush @09:53:13.08 CS.seek 3.000ml @09:53:20.74 delay 5 seconds @09:53:25.75 CSV.dial :air @09:53:26.23 CS.seek 5.000ml @09:53:26.59 AFV.select :carrierSolution @09:53:26.71 delay 2 seconds @09:53:28.72 AS.reconfigure ASconfig @09:53:28.75 AS.seek 1.000ml @09:53:31.59
CSV.dial :air @09:53:31.61 CS.seek 0.000ml @09:53:43.68 delay 7 seconds @09:53:50.69 CS.seek 3.000ml @09:53:58.40 CSV.dial :waste @09:53:59.20 CS.seek 0.000ml @09:54:06.80 Disinfecting external Intake with mfbkill @09:54:06.89 CSV.dial CSR @09:54:08.71 CSR.select :mfbkill @09:54:08.81 CS.seek 8.000ml @09:54:27.36 delay 1 second @09:54:27.99
delay 5 seconds @09:54:28.37 AFV.select :holdingCoil @09:54:28.46 delay 1 second @09:54:29.47 ATV.dial :ASV @09:54:29.48 ASV.dial :wasteR @09:54:29.49 AS.reconfigure ASslow1 @09:54:29.52 AS.seek 0.000ml @09:54:33.00
CSR.select :air @09:54:33.09 CS.seek 10.000ml @09:54:38.55 CSV.dial CBV @09:54:38.57
CBV.dial CSV @09:54:39.66 CTV.dial :intake @09:54:40.36 Intake.select :open @09:54:40.46 CS.seek 2.000ml @09:54:59.71 Intake.select :close @09:54:59.80 CTV.dial :air @09:55:00.54 CS.reconfigure CSconfig @09:55:00.57 CS.seek 0.000ml @09:55:06.46 Flushing lower manifold's waste1 port @09:55:06.47 CS Pulling 4ml of mfbkill @09:55:06.48 CSV.dial CSR @09:55:07.55 CSR.select :mfbkill @09:55:07.65 CS.seek 4.000ml @09:55:18.68 delay 5 seconds @09:55:23.69 CSR.select :air @09:55:23.78 CS.reconfigure CSfast @09:55:23.82 CS.seek 10.000ml @09:55:38.47 CSV.dial CTV @09:55:38.49
CTV.dial CSV @09:55:39.85 CBV.dial :waste1 @09:55:40.32 CS.seek 0.000ml @09:56:03.80 Flushing lower manifold's waste2b port @09:56:03.81 CS Pulling 4ml of mfbkill @09:56:03.82 CSV.dial CSR @09:56:04.34 CSR.select :mfbkill @09:56:04.44 CS.reconfigure CSconfig @09:56:04.48 CS.seek 4.000ml @09:56:15.49 delay 5 seconds @09:56:20.50 CSR.select :air @09:56:20.59 CS.reconfigure CSfast @09:56:20.63 CS.seek 10.000ml @09:56:35.06 CSV.dial CTV @09:56:35.08
CTV.dial CSV @09:56:35.60 CBV.dial :waste2b @09:56:36.34 CS.seek 0.000ml @09:56:59.67 Killing upper manifold @09:56:59.68 CS Pulling 4ml of mfbkill @09:56:59.69 CSV.dial CSR @09:57:00.59 CSR.select :mfbkill @09:57:00.69 CS.reconfigure CSconfig @09:57:00.73 CS.seek 4.000ml @09:57:11.76 delay 5 seconds @09:57:16.77 CSR.select :air @09:57:16.86 CS.reconfigure CSfast @09:57:16.90 CS.seek 10.000ml @09:57:31.45 CSV.dial CBV @09:57:31.47
CBV.dial CSV @09:57:32.50 CTV.dial :air @09:57:33.79 CS.seek 0.000ml @09:57:53.81 delay 20 seconds @09:57:57.07
delay 7 seconds @09:58:04.08 CS.seek 3.000ml @09:58:11.76 CSV.dial :waste @09:58:12.19 CS.seek 0.000ml @09:58:13.82 ATV.dial :air3 @09:58:14.93 AS.seek 0.590ml @09:58:19.79
Flushing upper manifold @09:58:19.88 CSV.dial :flush @09:58:20.54 CS.seek 5.000ml @09:58:32.73 delay 5 seconds @09:58:37.74 CSV.dial :air @09:58:38.31 CS.seek 7.000ml @09:58:43.67 CSV.dial CBV @09:58:43.69
CBV.dial CSV @09:58:45.37 CTV.dial :air @09:58:45.39 CS.seek 0.000ml @09:59:01.92 delay 7 seconds @09:59:08.93 CS.seek 3.000ml @09:59:16.57 CSV.dial :waste @09:59:17.00 CS.seek 0.000ml @09:59:24.75 CSV.dial :flush @09:59:25.17 CS.seek 3.000ml @09:59:32.88 delay 5 seconds @09:59:37.89 CSV.dial :air @09:59:38.37 CS.seek 5.001ml @09:59:43.76 CSV.dial :air @09:59:43.78 CS.seek 0.000ml @09:59:55.91 delay 7 seconds @10:00:02.92 CS.seek 3.000ml @10:00:10.57 CSV.dial :waste @10:00:11.37 CS.seek 0.000ml @10:00:15.55 delay 3 seconds @10:00:18.56 ATV.dial :ASV @10:00:19.13
Flushing lower manifold's waste1 port @10:00:19.22 CSV.dial :flush @10:00:19.63 ASV.dial :wasteR @10:00:19.69 AS.seek 0.090ml @10:00:20.69
CS.seek 5.000ml @10:00:32.91 delay 5 seconds @10:00:37.92 CSV.dial :air @10:00:38.41 CS.seek 7.000ml @10:00:43.82 CSV.dial CTV @10:00:43.84
CTV.dial CSV @10:00:45.18 CBV.dial :waste1 @10:00:45.65 CS.seek 0.000ml @10:01:02.25 Flushing lower manifold's waste2b port @10:01:02.34 CSV.dial :flush @10:01:03.15 CS.seek 5.000ml @10:01:15.28 delay 5 seconds @10:01:20.29 CSV.dial :air @10:01:20.85 CS.seek 6.999ml @10:01:26.26 CTV.dial CSV @10:01:26.27 CSV.dial CTV @10:01:26.70
CBV.dial :waste2b @10:01:27.45 CS.seek 0.000ml @10:01:43.91 Disinfecting external Intake with kill @10:01:44.00 CSV.dial CSR @10:01:44.42 CSR.select :kill @10:01:44.52 CS.seek 8.000ml @10:02:01.92 delay 30 seconds @10:02:03.41
delay 5 seconds @10:02:08.42 CSR.select :air @10:02:08.51 CS.seek 10.000ml @10:02:13.91 CSV.dial CBV @10:02:13.93
CBV.dial CSV @10:02:14.96 CTV.dial :intake @10:02:15.70 Intake.select :open @10:02:15.80 CS.seek 2.000ml @10:02:31.93 ATV.dial :extSrc @10:02:32.56 delay 3 seconds @10:02:34.87
Intake.select :close @10:02:34.96 CTV.dial :air @10:02:35.57 ATV.dial :ElutionWater @10:02:35.67
CS.reconfigure CSconfig @10:02:35.71 CS.seek 0.000ml @10:02:36.52 AS.seek 0.120ml @10:02:41.66
CSV.dial CSR @10:02:42.73 CSR.select :flush @10:02:42.83 CS.seek 5.000ml @10:02:42.91 ATV.dial :air3 @10:02:43.30 AS.seek 0.130ml @10:02:45.59 delay 2 seconds @10:02:47.60 ATV.dial :bleach @10:02:47.97 AS.seek 0.230ml @10:02:56.31
delay 5 seconds @10:03:01.32 CSR.select :air @10:03:01.42 CS.seek 10.000ml @10:03:08.67 delay 3 seconds @10:03:11.68 ATV.dial :air3 @10:03:12.03 delay 3 seconds @10:03:14.87
CSV.dial CBV @10:03:15.09 AS.seek 0.255ml @10:03:16.15
CS.seek 0.000ml @10:03:20.45 delay 3 seconds @10:03:23.46 ATV.dial :bleach @10:03:23.84 AS.seek 0.240ml @10:03:27.17 delay 3 seconds @10:03:30.18 ATV.dial :ASV @10:03:31.15 ASV.dial :SPEdelivery @10:03:31.51 AFV.select :carrierSolution @10:03:31.61 delay 2 seconds @10:03:33.62 AS.reconfigure ASconfig @10:03:33.65 AS.seek 0.500ml @10:03:42.28
Garaging FlushPuck in Collection Clamp @10:03:42.34 Elbow.reconfigure TightElbow @10:03:42.37 Elbow.seek CC @10:03:42.47 Elbow.reconfigure LooseElbow @10:03:42.55 Forearm.seek CC @10:03:43.49 CC.seek sealed @10:03:44.40 Hand.grip @10:03:45.09 CC.seek open @10:03:48.44 Forearm.seek retract @10:03:49.08 delay 1 second @10:03:49.38
Elbow.reconfigure TightElbow @10:03:49.41 Elbow.seek garage @10:03:50.09 AFV.select :holdingCoil @10:03:50.23 AS.reconfigure ASslow1 @10:03:50.26 AS.seek 0.170ml @10:03:52.25
Elbow.reconfigure LooseElbow @10:03:52.33 Forearm.reconfigure TightForearm @10:03:52.36 Forearm.seek garage @10:03:53.53 Hand.release @10:03:54.25 Forearm.reconfigure LooseForearm @10:03:54.28 Forearm.seek retract @10:04:57.81 SPE.hold 2:46:40,95.0C @10:04:57.83 delay 3:00 @10:06:37.85 Session Begun @10:06:42.57 -> showlog @10:07:57.85 AFV.select :carrierSolution @10:07:57.96 delay 2 seconds @10:07:59.97 AS.reconfigure ASconfig @10:08:00.02 AS.seek 1.000ml @10:08:48.72 delay 1 second @10:08:49.73 AFV.select :holdingCoil @10:08:49.83 delay 1 second @10:08:50.84 ASV.dial :wasteD @10:08:51.87 AS.seek 0.000ml @10:09:50.49 Priming Eluate @10:09:50.50 AFV.select :carrierSolution @10:09:50.61 delay 2 seconds @10:09:52.62 AS.seek 0.500ml @10:10:22.07 delay 1 second @10:10:23.08 AFV.select :holdingCoil @10:10:23.18 ATV.dial ASV @10:10:23.19 ASV.dial :SPErecovery @10:10:23.88 Swish #1 of 2 @10:10:23.95 AS.reconfigure ASslow0 @10:10:23.99 AS.seek 0.600ml @10:10:34.59 AS.seek 0.500ml @10:10:45.10 Swish #2 of 2 @10:10:45.17 AS.seek 0.600ml @10:10:55.71 AS.seek 0.500ml @10:11:06.20 delay 3 seconds @10:11:09.26 AS.reconfigure ASslow1 @10:11:09.30 AS.seek 0.676ml @10:11:45.48 delay 3 seconds @10:11:48.49 Eluate primed @10:11:48.50 ATV.dial :waste2 @10:11:49.14 AS.reconfigure ASnorm @10:11:49.18 AS.seek 0.000ml @10:12:24.07 SPE.coast @10:12:24.16 ASV.dial :SPErecovery @10:12:24.18 Setting up PCR for HF183em -- writing /var/log/esp/HF183em14mar1710h400ml.pcr @10:12:24.20 Building PCR Slug from ASV with MM2 mix, PP4 primer @10:12:24.21 Priming with carrier @10:12:24.23 ATV.dial ARV @10:12:24.93 ARV.dial :carrier @10:12:25.23 AS.seek 0.500ml @10:12:51.10 AFV.select :carrierSolution @10:12:51.20 delay 2 seconds @10:12:53.21 AS.reconfigure ASconfig @10:12:53.26 AS.seek 1.000ml @10:13:22.72 delay 1 second @10:13:23.73 AFV.select :holdingCoil @10:13:23.83 delay 1 second @10:13:24.84 ATV.dial :waste @10:13:26.12 AS.reconfigure ASnorm @10:13:26.17 AS.seek empty @10:14:17.66 AFV.select :carrierSolution @10:14:17.77 delay 2 seconds @10:14:19.78 AS.reconfigure ASconfig @10:14:19.83 AS.seek 0.500ml @10:14:49.33 delay 1 second @10:14:50.34 AFV.select :holdingCoil @10:14:50.45 Building Slug @10:14:50.46 ATV.dial :ARV @10:14:51.76 delay 2 seconds @10:14:53.76 ARV.dial :air1 @10:14:54.09 delay 2 seconds @10:14:56.15 AS.reconfigure ASslow2 @10:14:56.20 AS.seek 0.506ml @10:15:02.73 delay 2 seconds @10:15:04.75 ARV.dial :MM2 @10:15:06.08 delay 2 seconds @10:15:08.09 Adding MM2 @10:15:08.16 AS.seek 0.524ml @10:15:26.77 delay 25 seconds @10:15:51.78 ARV.dial :PP4 @10:15:52.12 Adding PP4 @10:15:52.14 delay 2 seconds @10:15:54.19 AS.seek 0.530ml @10:16:00.44 delay 10 seconds @10:16:10.45 ARV.dial :air3 @10:16:10.87 adding ARV air @10:16:10.88 delay 2 seconds @10:16:12.94 AS.seek 0.536ml @10:16:19.20 delay 5 seconds @10:16:24.22 ARV.dial :carrier @10:16:25.26 delay 7 seconds @10:16:32.32 AS.seek 0.594ml @10:17:31.64 delay 5 seconds @10:17:36.66 ATV.dial :ASV @10:17:37.09 delay 2 seconds @10:17:39.15 AS.seek 0.600ml @10:17:45.50 delay 4 seconds @10:17:49.52 ATV.dial ARV @10:17:49.95 delay 2 seconds @10:17:51.96 ARV.dial :carrier @10:17:51.97 delay 2 seconds @10:17:54.03 AS.seek 0.650ml @10:18:45.56 delay 4 seconds @10:18:49.58 ATV.dial PCR @10:18:50.10 ASV.dial :waste1 @10:18:51.06 ARV.dial :air1 @10:18:51.42 Positioning Slug by 0.324ml @10:18:51.49 AS.seek 0.326ml @10:24:22.00 delay 5 seconds @10:24:27.01 ATV.dial ARV @10:24:27.54 ASV.dial :PCRlock @10:24:28.31 delay 5 seconds @10:24:33.32 Running HF183em ProfileEM60C2 thermocycling @10:24:33.33 PCR.program ProfileEM60C2 @10:24:33.46 ProfileEM60C2 should complete in < 2:11:38 @10:24:33.47 PCR.run 2:49:32.5 @12:20:51.49 PCR.off @12:20:52.02 PCR for HF183em completed -- closed /var/log/esp/HF183em14mar1710h400ml.pcr @12:20:52.08 Cleaning PCR AFV.select :carrierSolution @12:20:52.19 delay 2 seconds @12:20:54.20 AS.reconfigure ASconfig @12:20:54.25 AS.seek 0.500ml @12:21:04.71 delay 1 second @12:21:05.72 AFV.select :holdingCoil @12:21:05.82 ASV.dial :waste1 @12:21:06.41 ATV.dial :air3 @12:21:07.37 AS.reconfigure ASslow1 @12:21:07.42 AS.seek 0.510ml @12:21:09.74 delay 2 seconds @12:21:11.75 ATV.dial :bleach @12:21:12.24 AS.seek 0.810ml @12:22:13.67 delay 3 seconds @12:22:16.68 ATV.dial :air3 @12:22:17.13 delay 3 seconds @12:22:20.19 AS.seek 0.835ml @12:22:25.55 delay 3 seconds @12:22:28.56 ATV.dial :bleach @12:22:28.96 AS.seek 0.820ml @12:22:32.30 delay 3 seconds @12:22:35.31 ARV.dial :waste @12:22:35.81 delay 3 seconds @12:22:38.82 ATV.dial :ARV @12:22:39.57 AS.reconfigure ASconfig @12:22:39.62 AS.seek 0.470ml @12:23:00.33 AFV.select :carrierSolution @12:23:00.44 delay 2 seconds @12:23:02.45 AS.seek 1.000ml @12:23:33.70 delay 1 second @12:23:34.71 AFV.select :holdingCoil @12:23:34.81 delay 1 second @12:23:35.82 AS.reconfigure ASfast @12:23:35.87 AS.seek 0.000ml @12:24:03.62 AFV.select :carrierSolution @12:24:03.73 delay 2 seconds @12:24:05.74 AS.reconfigure ASconfig @12:24:05.79 AS.seek 1.000ml @12:25:04.41 delay 1 second @12:25:05.42 AFV.select :holdingCoil @12:25:05.52 delay 1 second @12:25:06.53 AS.reconfigure ASfast @12:25:06.58 AS.seek 0.000ml @12:25:34.33 AFV.select :carrierSolution @12:25:34.44 delay 2 seconds @12:25:36.45 AS.reconfigure ASconfig @12:25:36.50 AS.seek 1.000ml @12:26:35.11 delay 1 second @12:26:36.12 AFV.select :holdingCoil @12:26:36.23 delay 1 second @12:26:37.24 AS.reconfigure ASfast @12:26:37.29 AS.seek 0.000ml @12:27:05.05 ARV.dial between carrier and air1 @12:27:05.47 ATV.dial :air3 @12:27:06.43 AS.reconfigure ASslow1 @12:27:06.48 AS.seek 0.010ml @12:27:08.85 delay 2 seconds @12:27:10.86 ATV.dial :bleach @12:27:11.26 AS.seek 0.810ml @12:29:54.67 delay 3 seconds @12:29:57.68 ATV.dial :air3 @12:29:58.03 delay 3 seconds @12:30:01.09 AS.seek 0.835ml @12:30:06.45 delay 3 seconds @12:30:09.46 ATV.dial :bleach @12:30:09.86 AS.seek 0.820ml @12:30:13.18 delay 3 seconds @12:30:16.76 ATV.dial PCR @12:30:17.08 AS.reconfigure ASconfig @12:30:17.12 AS.seek empty @12:31:05.26 PCR.program :bleachProfile @12:31:05.32 PCR program should complete in < 2:45 @12:31:05.33 PCR.run 10:00 @12:34:03.53 PCR.off @12:34:03.57 AFV.select :carrierSolution @12:34:03.67 delay 2 seconds @12:34:05.68 AS.seek 1.000ml @12:35:04.33 delay 1 second @12:35:05.35 AFV.select :holdingCoil @12:35:05.46 delay 1 second @12:35:06.47 AS.reconfigure ASfast @12:35:06.52 AS.seek 0.000ml @12:35:34.28 delay 3 seconds @12:35:37.29 AFV.select :carrierSolution @12:35:37.39 delay 2 seconds @12:35:39.40 AS.reconfigure ASconfig @12:35:39.45 AS.seek 1.000ml @12:36:38.08 delay 1 second @12:36:39.10 AFV.select :holdingCoil @12:36:39.24 delay 1 second @12:36:40.25 AS.reconfigure ASfast @12:36:40.30 AS.seek 0.000ml @12:37:08.05 delay 3 seconds @12:37:11.06 AFV.select :carrierSolution @12:37:11.16 delay 2 seconds @12:37:13.17 AS.reconfigure ASconfig @12:37:13.22 AS.seek 1.000ml @12:38:11.84 delay 1 second @12:38:12.85 AFV.select :holdingCoil @12:38:12.95 delay 1 second @12:38:13.96 AS.reconfigure ASfast @12:38:14.01 AS.seek 0.000ml @12:38:41.78 delay 3 seconds @12:38:44.79 AFV.select :carrierSolution @12:38:44.89 delay 2 seconds @12:38:46.90 AS.reconfigure ASconfig @12:38:46.95 AS.seek 1.000ml @12:39:45.57 delay 1 second @12:39:46.58 AFV.select :holdingCoil @12:39:46.68 delay 1 second @12:39:47.69 AS.reconfigure ASfast @12:39:47.74 AS.seek 0.000ml @12:40:15.46 delay 3 seconds @12:40:18.47 AFV.select :carrierSolution @12:40:18.57 delay 2 seconds @12:40:20.58 AS.reconfigure ASconfig @12:40:20.63 AS.seek 1.000ml @12:41:19.25 delay 1 second @12:41:20.26 AFV.select :holdingCoil @12:41:20.36 delay 1 second @12:41:21.37 AS.reconfigure ASnorm @12:41:21.42 AS.seek 0.000ml @12:42:12.92 Setting up PCR for ENTap -- writing /var/log/esp/ENTap14mar1712h400ml.pcr @12:42:12.93 Building PCR Slug from ASV with MM1 mix, PP1 primer @12:42:12.94 Priming with carrier @12:42:12.95 ATV.dial ARV @12:42:13.50 ARV.dial :carrier @12:42:13.80 AS.seek 0.500ml @12:42:39.70 AFV.select :carrierSolution @12:42:39.81 delay 2 seconds @12:42:41.82 AS.reconfigure ASconfig @12:42:41.87 AS.seek 1.000ml @12:43:11.31 delay 1 second @12:43:12.32 AFV.select :holdingCoil @12:43:12.42 delay 1 second @12:43:13.43 ATV.dial :waste @12:43:14.80 AS.reconfigure ASnorm @12:43:14.85 AS.seek empty @12:44:06.34 AFV.select :carrierSolution @12:44:06.44 delay 2 seconds @12:44:08.45 AS.reconfigure ASconfig @12:44:08.50 AS.seek 0.500ml @12:44:37.98 delay 1 second @12:44:38.99 AFV.select :holdingCoil @12:44:39.09 Building Slug @12:44:39.10 ATV.dial :ARV @12:44:40.37 delay 2 seconds @12:44:42.38 ARV.dial :air1 @12:44:42.73 delay 2 seconds @12:44:44.79 AS.reconfigure ASslow2 @12:44:44.84 AS.seek 0.506ml @12:44:51.16 delay 2 seconds @12:44:53.17 ARV.dial :MM1 @12:44:53.68 delay 2 seconds @12:44:55.69 Adding MM1 @12:44:55.75 AS.seek 0.524ml @12:45:14.24 delay 25 seconds @12:45:39.25 ARV.dial :PP1 @12:45:39.60 Adding PP1 @12:45:39.61 delay 2 seconds @12:45:41.67 AS.seek 0.530ml @12:45:48.18 delay 10 seconds @12:45:58.19 ARV.dial :air1 @12:45:58.53 adding ARV air @12:45:58.54 delay 2 seconds @12:46:00.60 AS.seek 0.536ml @12:46:06.91 delay 5 seconds @12:46:11.93 ARV.dial :carrier @12:46:12.26 delay 7 seconds @12:46:19.32 AS.seek 0.594ml @12:47:18.64 delay 5 seconds @12:47:23.66 ATV.dial :ASV @12:47:24.00 delay 2 seconds @12:47:26.06 AS.seek 0.600ml @12:47:32.39 delay 4 seconds @12:47:36.41 ATV.dial ARV @12:47:36.75 delay 2 seconds @12:47:38.76 ARV.dial :carrier @12:47:38.77 delay 2 seconds @12:47:40.83 AS.seek 0.650ml @12:48:32.20 delay 4 seconds @12:48:36.22 ATV.dial PCR @12:48:36.81 ASV.dial :waste1 @12:48:36.82 ARV.dial :air1 @12:48:37.18 Positioning Slug by 0.324ml @12:48:37.25 AS.seek 0.326ml @12:54:07.76 delay 5 seconds @12:54:12.78 ATV.dial ARV @12:54:13.81 ASV.dial :PCRlock @12:54:14.26 delay 5 seconds @12:54:19.27 Running ENTap Profile60C thermocycling @12:54:19.28 PCR.program Profile60C @12:54:19.40 Profile60C should complete in < 1:39:08 @12:54:19.41 PCR.run 2:08:55 @14:17:44.32 PCR.off @14:17:44.83 PCR for ENTap completed -- closed /var/log/esp/ENTap14mar1712h400ml.pcr @14:17:44.90 Cleaning PCR AFV.select :carrierSolution @14:17:45.01 delay 2 seconds @14:17:47.02 AS.reconfigure ASconfig @14:17:47.07 AS.seek 0.500ml @14:17:57.53 delay 1 second @14:17:58.54 AFV.select :holdingCoil @14:17:58.64 ASV.dial :waste1 @14:17:59.11 ATV.dial :air3 @14:18:00.05 AS.reconfigure ASslow1 @14:18:00.10 AS.seek 0.510ml @14:18:02.39 delay 2 seconds @14:18:04.40 ATV.dial :bleach @14:18:04.78 AS.seek 0.810ml @14:19:06.22 delay 3 seconds @14:19:09.23 ATV.dial :air3 @14:19:09.65 delay 3 seconds @14:19:12.71 AS.seek 0.835ml @14:19:18.06 delay 3 seconds @14:19:21.07 ATV.dial :bleach @14:19:21.45 AS.seek 0.820ml @14:19:24.76 delay 3 seconds @14:19:27.77 ARV.dial :waste @14:19:28.37 delay 3 seconds @14:19:31.39 ATV.dial :ARV @14:19:32.15 AS.reconfigure ASconfig @14:19:32.19 AS.seek 0.470ml @14:19:52.89 AFV.select :carrierSolution @14:19:53.00 delay 2 seconds @14:19:55.01 AS.seek 1.000ml @14:20:26.23 delay 1 second @14:20:27.24 AFV.select :holdingCoil @14:20:27.34 delay 1 second @14:20:28.35 AS.reconfigure ASfast @14:20:28.39 AS.seek 0.000ml @14:20:56.13 AFV.select :carrierSolution @14:20:56.24 delay 2 seconds @14:20:58.25 AS.reconfigure ASconfig @14:20:58.30 AS.seek 1.000ml @14:21:56.93 delay 1 second @14:21:57.94 AFV.select :holdingCoil @14:21:58.04 delay 1 second @14:21:59.05 AS.reconfigure ASfast @14:21:59.10 AS.seek 0.000ml @14:22:26.83 AFV.select :carrierSolution @14:22:26.94 delay 2 seconds @14:22:28.95 AS.reconfigure ASconfig @14:22:29.00 AS.seek 1.000ml @14:23:27.64 delay 1 second @14:23:28.65 AFV.select :holdingCoil @14:23:29.31 delay 1 second @14:23:30.32 AS.reconfigure ASfast @14:23:30.37 AS.seek 0.000ml @14:23:58.11 ARV.dial between carrier and air1 @14:23:58.53 ATV.dial :air3 @14:23:59.48 AS.reconfigure ASslow1 @14:23:59.53 AS.seek 0.010ml @14:24:01.87 delay 2 seconds @14:24:03.88 ATV.dial :bleach @14:24:04.37 AS.seek 0.810ml @14:26:47.77 delay 3 seconds @14:26:50.78 ATV.dial :air3 @14:26:51.12 delay 3 seconds @14:26:54.18 AS.seek 0.835ml @14:26:59.52 delay 3 seconds @14:27:02.54 ATV.dial :bleach @14:27:03.02 AS.seek 0.820ml @14:27:06.33 delay 3 seconds @14:27:09.34 ATV.dial PCR @14:27:09.69 AS.reconfigure ASconfig @14:27:09.73 AS.seek empty @14:27:57.86 PCR.program :bleachProfile @14:27:57.92 PCR program should complete in < 2:45 @14:27:57.93 PCR.run 10:00 @14:30:56.13 PCR.off @14:30:56.17 AFV.select :carrierSolution @14:30:56.28 delay 2 seconds @14:30:58.29 AS.seek 1.000ml @14:31:56.95 delay 1 second @14:31:57.96 AFV.select :holdingCoil @14:31:58.06 delay 1 second @14:31:59.07 AS.reconfigure ASfast @14:31:59.12 AS.seek 0.000ml @14:32:26.85 delay 3 seconds @14:32:29.86 AFV.select :carrierSolution @14:32:29.96 delay 2 seconds @14:32:31.97 AS.reconfigure ASconfig @14:32:32.02 AS.seek 1.000ml @14:33:30.65 delay 1 second @14:33:31.66 AFV.select :holdingCoil @14:33:31.76 delay 1 second @14:33:32.77 AS.reconfigure ASfast @14:33:32.81 AS.seek 0.000ml @14:34:00.54 delay 3 seconds @14:34:03.55 AFV.select :carrierSolution @14:34:03.65 delay 2 seconds @14:34:05.66 AS.reconfigure ASconfig @14:34:05.71 AS.seek 1.000ml @14:35:04.33 delay 1 second @14:35:05.34 AFV.select :holdingCoil @14:35:05.44 delay 1 second @14:35:06.45 AS.reconfigure ASfast @14:35:06.50 AS.seek 0.000ml @14:35:34.25 delay 3 seconds @14:35:37.26 AFV.select :carrierSolution @14:35:37.36 delay 2 seconds @14:35:39.37 AS.reconfigure ASconfig @14:35:39.42 AS.seek 1.000ml @14:36:38.03 delay 1 second @14:36:39.04 AFV.select :holdingCoil @14:36:39.14 delay 1 second @14:36:40.15 AS.reconfigure ASfast @14:36:40.19 AS.seek 0.000ml @14:37:07.94 delay 3 seconds @14:37:10.95 AFV.select :carrierSolution @14:37:11.05 delay 2 seconds @14:37:13.06 AS.reconfigure ASconfig @14:37:13.11 AS.seek 1.000ml @14:38:11.72 delay 1 second @14:38:12.73 AFV.select :holdingCoil @14:38:12.83 delay 1 second @14:38:13.84 AS.reconfigure ASnorm @14:38:13.89 AS.seek 0.000ml @14:39:05.36 Setting up PCR for ENTem -- writing /var/log/esp/ENTem14mar1714h400ml.pcr @14:39:05.37 Building PCR Slug from ASV with MM2 mix, PP3 primer @14:39:05.38 Priming with carrier @14:39:05.40 ATV.dial ARV @14:39:05.94 ARV.dial :carrier @14:39:06.24 AS.seek 0.500ml @14:39:32.18 AFV.select :carrierSolution @14:39:32.29 delay 2 seconds @14:39:34.30 AS.reconfigure ASconfig @14:39:34.35 AS.seek 1.000ml @14:40:03.79 delay 1 second @14:40:04.80 AFV.select :holdingCoil @14:40:04.90 delay 1 second @14:40:05.91 ATV.dial :waste @14:40:07.18 AS.reconfigure ASnorm @14:40:07.22 AS.seek empty @14:40:58.71 AFV.select :carrierSolution @14:40:58.82 delay 2 seconds @14:41:00.83 AS.reconfigure ASconfig @14:41:00.87 AS.seek 0.500ml @14:41:30.36 delay 1 second @14:41:31.37 AFV.select :holdingCoil @14:41:31.47 Building Slug @14:41:31.48 ATV.dial :ARV @14:41:32.82 delay 2 seconds @14:41:34.83 ARV.dial :air1 @14:41:35.17 delay 2 seconds @14:41:37.23 AS.reconfigure ASslow2 @14:41:37.28 AS.seek 0.506ml @14:41:43.53 delay 2 seconds @14:41:45.55 ARV.dial :MM2 @14:41:46.77 delay 2 seconds @14:41:48.78 Adding MM2 @14:41:48.85 AS.seek 0.524ml @14:42:07.53 delay 25 seconds @14:42:32.54 ARV.dial :PP3 @14:42:32.96 Adding PP3 @14:42:32.97 delay 2 seconds @14:42:35.03 AS.seek 0.530ml @14:42:41.39 delay 10 seconds @14:42:51.40 ARV.dial :air2 @14:42:51.82 adding ARV air @14:42:51.83 delay 2 seconds @14:42:53.88 AS.seek 0.536ml @14:43:00.12 delay 5 seconds @14:43:05.13 ARV.dial :carrier @14:43:06.17 delay 7 seconds @14:43:13.23 AS.seek 0.594ml @14:44:12.51 delay 5 seconds @14:44:18.03 ATV.dial :ASV @14:44:18.37 delay 2 seconds @14:44:20.43 AS.seek 0.600ml @14:44:26.79 delay 4 seconds @14:44:30.80 ATV.dial ARV @14:44:31.25 delay 2 seconds @14:44:33.26 ARV.dial :carrier @14:44:33.27 delay 2 seconds @14:44:35.33 AS.seek 0.650ml @14:45:26.65 delay 4 seconds @14:45:30.66 ATV.dial PCR @14:45:31.18 ASV.dial :waste1 @14:45:31.19 ARV.dial :air1 @14:45:31.55 Positioning Slug by 0.324ml @14:45:31.62 AS.seek 0.326ml @14:51:02.25 delay 5 seconds @14:51:07.26 ATV.dial ARV @14:51:07.80 ASV.dial :PCRlock @14:51:08.30 delay 5 seconds @14:51:13.31 Running ENTem ProfileEM60C thermocycling @14:51:13.32 PCR.program ProfileEM60C @14:51:13.45 ProfileEM60C should complete in < 2:11:38 @14:51:13.46 PCR.run 2:49:32.5 @16:48:06.72 PCR.off @16:48:07.26 PCR for ENTem completed -- closed /var/log/esp/ENTem14mar1714h400ml.pcr @16:48:07.32 Cleaning PCR @16:48:07.33 AFV.select :carrierSolution @16:48:07.43 delay 2 seconds @16:48:09.44 AS.reconfigure ASconfig @16:48:09.49 AS.seek 0.500ml @16:48:19.93 delay 1 second @16:48:20.94 AFV.select :holdingCoil @16:48:21.04 ASV.dial :waste1 @16:48:21.54 ATV.dial :air3 @16:48:22.49 AS.reconfigure ASslow1 @16:48:22.54 AS.seek 0.510ml @16:48:24.86 delay 2 seconds @16:48:26.87 ATV.dial :bleach @16:48:27.27 AS.seek 0.810ml @16:49:28.69 delay 3 seconds @16:49:31.70 ATV.dial :air3 @16:49:32.05 delay 3 seconds @16:49:35.11 AS.seek 0.835ml @16:49:40.47 delay 3 seconds @16:49:43.48 ATV.dial :bleach @16:49:43.88 AS.seek 0.820ml @16:49:47.75 delay 3 seconds @16:49:50.76 ARV.dial :waste @16:49:51.36 delay 3 seconds @16:49:54.37 ATV.dial :ARV @16:49:55.11 AS.reconfigure ASconfig @16:49:55.15 AS.seek 0.470ml @16:50:15.83 AFV.select :carrierSolution @16:50:15.94 delay 2 seconds @16:50:17.95 AS.seek 1.000ml @16:50:49.19 delay 1 second @16:50:50.20 AFV.select :holdingCoil @16:50:50.30 delay 1 second @16:50:51.31 AS.reconfigure ASfast @16:50:51.36 AS.seek 0.000ml @16:51:19.12 AFV.select :carrierSolution @16:51:19.23 delay 2 seconds @16:51:21.24 AS.reconfigure ASconfig @16:51:21.29 AS.seek 1.000ml @16:52:19.92 delay 1 second @16:52:20.94 AFV.select :holdingCoil @16:52:21.04 delay 1 second @16:52:22.05 AS.reconfigure ASfast @16:52:22.10 AS.seek 0.000ml @16:52:49.84 AFV.select :carrierSolution @16:52:49.95 delay 2 seconds @16:52:51.96 AS.reconfigure ASconfig @16:52:52.01 AS.seek 1.000ml @16:53:50.63 delay 1 second @16:53:51.64 AFV.select :holdingCoil @16:53:51.74 delay 1 second @16:53:52.75 AS.reconfigure ASfast @16:53:52.80 AS.seek 0.000ml @16:54:20.55 ARV.dial between carrier and air1 @16:54:20.98 ATV.dial :air3 @16:54:22.01 AS.reconfigure ASslow1 @16:54:22.06 AS.seek 0.010ml @16:54:24.41 delay 2 seconds @16:54:26.42 ATV.dial :bleach @16:54:26.92 AS.seek 0.810ml @16:57:10.32 delay 3 seconds @16:57:13.33 ATV.dial :air3 @16:57:13.67 delay 3 seconds @16:57:16.87 AS.seek 0.835ml @16:57:22.21 delay 3 seconds @16:57:25.22 ATV.dial :bleach @16:57:25.72 AS.seek 0.820ml @16:57:29.10 delay 3 seconds @16:57:32.11 ATV.dial PCR @16:57:32.46 AS.reconfigure ASconfig @16:57:32.50 AS.seek empty @16:58:20.62 PCR.program :bleachProfile @16:58:20.68 PCR program should complete in < 2:45 @16:58:20.69 PCR.run 10:00 @17:01:18.90 PCR.off @17:01:18.93 AFV.select :carrierSolution @17:01:19.04 delay 2 seconds @17:01:21.05 AS.seek 1.000ml @17:02:19.70 delay 1 second @17:02:20.71 AFV.select :holdingCoil @17:02:20.81 delay 1 second @17:02:21.82 AS.reconfigure ASfast @17:02:22.00 AS.seek 0.000ml @17:02:49.75 delay 3 seconds @17:02:52.76 AFV.select :carrierSolution @17:02:52.86 delay 2 seconds @17:02:54.87 AS.reconfigure ASconfig @17:02:54.92 AS.seek 1.000ml @17:03:53.54 delay 1 second @17:03:54.55 AFV.select :holdingCoil @17:03:54.66 delay 1 second @17:03:55.67 AS.reconfigure ASfast @17:03:55.71 AS.seek 0.000ml @17:04:23.43 delay 3 seconds @17:04:26.44 AFV.select :carrierSolution @17:04:26.54 delay 2 seconds @17:04:28.55 AS.reconfigure ASconfig @17:04:28.60 AS.seek 1.000ml @17:05:27.21 delay 1 second @17:05:28.22 AFV.select :holdingCoil @17:05:28.32 delay 1 second @17:05:29.33 AS.reconfigure ASfast @17:05:29.38 AS.seek 0.000ml @17:05:57.10 delay 3 seconds @17:06:00.11 AFV.select :carrierSolution @17:06:00.21 delay 2 seconds @17:06:02.22 AS.reconfigure ASconfig @17:06:02.27 AS.seek 1.000ml @17:07:00.88 delay 1 second @17:07:01.89 AFV.select :holdingCoil @17:07:02.06 delay 1 second @17:07:03.07 AS.reconfigure ASfast @17:07:03.12 AS.seek 0.000ml @17:07:30.87 delay 3 seconds @17:07:33.88 AFV.select :carrierSolution @17:07:33.98 delay 2 seconds @17:07:35.99 AS.reconfigure ASconfig @17:07:36.03 AS.seek 1.000ml @17:08:34.66 delay 1 second @17:08:35.67 AFV.select :holdingCoil @17:08:35.77 delay 1 second @17:08:36.78 AS.reconfigure ASnorm @17:08:36.83 AS.seek 0.000ml @17:09:28.30 Setting up PCR for IPCap -- writing /var/log/esp/IPCap14mar1717h400ml.pcr @17:09:28.31 Building PCR Slug from ASV with MM1 mix, PP2 primer @17:09:28.32 Priming with carrier @17:09:28.33 ATV.dial ARV @17:09:28.86 ARV.dial :carrier @17:09:29.16 AS.seek 0.500ml @17:09:55.05 AFV.select :carrierSolution @17:09:55.16 delay 2 seconds @17:09:57.17 AS.reconfigure ASconfig @17:09:57.22 AS.seek 1.000ml @17:10:26.67 delay 1 second @17:10:27.68 AFV.select :holdingCoil @17:10:27.78 delay 1 second @17:10:28.79 ATV.dial :waste @17:10:30.06 AS.reconfigure ASnorm @17:10:30.11 AS.seek empty @17:11:21.58 AFV.select :carrierSolution @17:11:21.69 delay 2 seconds @17:11:23.70 AS.reconfigure ASconfig @17:11:23.75 AS.seek 0.500ml @17:11:53.23 delay 1 second @17:11:54.24 AFV.select :holdingCoil @17:11:54.34 Building Slug @17:11:54.35 ATV.dial :ARV @17:11:55.70 delay 2 seconds @17:11:57.71 ARV.dial :air1 @17:11:58.04 delay 2 seconds @17:12:00.62 AS.reconfigure ASslow2 @17:12:00.67 AS.seek 0.506ml @17:12:06.92 delay 2 seconds @17:12:08.93 ARV.dial :MM1 @17:12:09.53 delay 2 seconds @17:12:11.54 Adding MM1 @17:12:11.61 AS.seek 0.524ml @17:12:30.32 delay 25 seconds @17:12:55.33 ARV.dial :PP2 @17:12:55.68 Adding PP2 @17:12:55.69 delay 2 seconds @17:12:57.74 AS.seek 0.530ml @17:13:04.09 delay 10 seconds @17:13:14.10 ARV.dial :air2 @17:13:14.54 adding ARV air @17:13:14.56 delay 2 seconds @17:13:16.61 AS.seek 0.536ml @17:13:22.89 delay 5 seconds @17:13:27.90 ARV.dial :carrier @17:13:28.93 delay 7 seconds @17:13:35.99 AS.seek 0.594ml @17:14:35.31 delay 5 seconds @17:14:40.32 ATV.dial :ASV @17:14:40.66 delay 2 seconds @17:14:42.72 AS.seek 0.600ml @17:14:49.06 delay 4 seconds @17:14:53.07 ATV.dial ARV @17:14:53.41 delay 2 seconds @17:14:55.42 ARV.dial :carrier @17:14:55.43 delay 2 seconds @17:14:57.49 AS.seek 0.650ml @17:15:48.84 delay 4 seconds @17:15:52.85 ATV.dial PCR @17:15:53.36 ASV.dial :waste1 @17:15:53.37 ARV.dial :air1 @17:15:53.73 Positioning Slug by 0.324ml @17:15:53.80 AS.seek 0.326ml @17:16:45.91 Session Ended @17:21:24.49 delay 5 seconds @17:21:29.50 ATV.dial ARV @17:21:30.02 ASV.dial :PCRlock @17:21:30.50 delay 5 seconds @17:21:35.51 Running IPCap Profile59C thermocycling @17:21:35.52 PCR.program Profile59C @17:21:35.62 Profile59C should complete in < 1:39:08 @17:21:35.63 PCR.run 2:08:55 @18:46:03.12 PCR.off @18:46:04.21 PCR for IPCap completed -- closed /var/log/esp/IPCap14mar1717h400ml.pcr @18:46:04.26 Cleaning PCR @18:46:04.27 AFV.select :carrierSolution @18:46:04.36 delay 2 seconds @18:46:06.37 AS.reconfigure ASconfig @18:46:06.41 AS.seek 0.500ml @18:46:16.84 delay 1 second @18:46:17.85 AFV.select :holdingCoil @18:46:17.94 ASV.dial :waste1 @18:46:18.70 ATV.dial :air3 @18:46:19.64 AS.reconfigure ASslow1 @18:46:19.68 AS.seek 0.510ml @18:46:21.98 delay 2 seconds @18:46:23.99 ATV.dial :bleach @18:46:24.37 AS.seek 0.810ml @18:47:25.79 delay 3 seconds @18:47:28.80 ATV.dial :air3 @18:47:29.15 delay 3 seconds @18:47:32.21 AS.seek 0.835ml @18:47:37.54 delay 3 seconds @18:47:40.55 ATV.dial :bleach @18:47:40.95 AS.seek 0.820ml @18:47:44.27 delay 3 seconds @18:47:47.28 ARV.dial :waste @18:47:47.87 delay 3 seconds @18:47:50.88 ATV.dial :ARV @18:47:51.63 AS.reconfigure ASconfig @18:47:51.66 AS.seek 0.470ml @18:48:12.35 AFV.select :carrierSolution @18:48:12.45 delay 2 seconds @18:48:14.46 AS.seek 1.000ml @18:48:45.66 delay 1 second @18:48:46.67 AFV.select :holdingCoil @18:48:46.76 delay 1 second @18:48:47.77 AS.reconfigure ASfast @18:48:47.80 AS.seek 0.000ml @18:49:15.50 AFV.select :carrierSolution @18:49:15.60 delay 2 seconds @18:49:17.61 AS.reconfigure ASconfig @18:49:17.64 AS.seek 1.000ml @18:50:16.19 delay 1 second @18:50:17.20 AFV.select :holdingCoil @18:50:17.29 delay 1 second @18:50:18.30 AS.reconfigure ASfast @18:50:18.33 AS.seek 0.000ml @18:50:46.05 AFV.select :carrierSolution @18:50:46.15 delay 2 seconds @18:50:48.16 AS.reconfigure ASconfig @18:50:48.19 AS.seek 1.000ml @18:51:46.78 delay 1 second @18:51:47.79 AFV.select :holdingCoil @18:51:47.88 delay 1 second @18:51:48.89 AS.reconfigure ASfast @18:51:48.92 AS.seek 0.000ml @18:52:16.62 ARV.dial between carrier and air1 @18:52:17.03 ATV.dial :air3 @18:52:17.95 AS.reconfigure ASslow1 @18:52:17.98 AS.seek 0.010ml @18:52:20.23 delay 2 seconds @18:52:22.24 ATV.dial :bleach @18:52:22.61 AS.seek 0.810ml @18:55:06.01 delay 3 seconds @18:55:09.02 ATV.dial :air3 @18:55:09.37 delay 3 seconds @18:55:12.43 AS.seek 0.835ml @18:55:17.85 delay 3 seconds @18:55:20.86 ATV.dial :bleach @18:55:21.24 AS.seek 0.820ml @18:55:24.54 delay 3 seconds @18:55:27.55 ATV.dial PCR @18:55:27.88 AS.reconfigure ASconfig @18:55:27.91 AS.seek empty @18:56:16.05 PCR.program :bleachProfile @18:56:16.10 PCR program should complete in < 2:45 @18:56:16.11 PCR.run 10:00 @18:59:14.30 PCR.off @18:59:14.33 AFV.select :carrierSolution @18:59:14.42 delay 2 seconds @18:59:16.43 AS.seek 1.000ml @19:00:15.10 delay 1 second @19:00:16.11 AFV.select :holdingCoil @19:00:16.20 delay 1 second @19:00:17.21 AS.reconfigure ASfast @19:00:17.25 AS.seek 0.000ml @19:00:44.97 delay 3 seconds @19:00:47.98 AFV.select :carrierSolution @19:00:48.07 delay 2 seconds @19:00:50.08 AS.reconfigure ASconfig @19:00:50.11 AS.seek 1.000ml @19:01:48.72 delay 1 second @19:01:49.73 AFV.select :holdingCoil @19:01:49.82 delay 1 second @19:01:50.83 AS.reconfigure ASfast @19:01:50.86 AS.seek 0.000ml @19:02:18.58 delay 3 seconds @19:02:21.59 AFV.select :carrierSolution @19:02:21.68 delay 2 seconds @19:02:23.69 AS.reconfigure ASconfig @19:02:23.72 AS.seek 1.000ml @19:03:22.33 delay 1 second @19:03:23.34 AFV.select :holdingCoil @19:03:23.43 delay 1 second @19:03:24.44 AS.reconfigure ASfast @19:03:24.47 AS.seek 0.000ml @19:03:52.20 delay 3 seconds @19:03:55.21 AFV.select :carrierSolution @19:03:55.30 delay 2 seconds @19:03:57.31 AS.reconfigure ASconfig @19:03:57.34 AS.seek 1.000ml @19:04:55.94 delay 1 second @19:04:56.95 AFV.select :holdingCoil @19:04:57.13 delay 1 second @19:04:58.14 AS.reconfigure ASfast @19:04:58.17 AS.seek 0.000ml @19:05:25.90 delay 3 seconds @19:05:28.91 AFV.select :carrierSolution @19:05:29.00 delay 2 seconds @19:05:31.01 AS.reconfigure ASconfig @19:05:31.04 AS.seek 1.000ml @19:06:29.65 delay 1 second @19:06:30.66 AFV.select :holdingCoil @19:06:30.75 delay 1 second @19:06:31.76 AS.reconfigure ASnorm @19:06:31.79 AS.seek 0.000ml @19:07:23.24 Setting up PCR for IPCem -- writing /var/log/esp/IPCem14mar1719h400ml.pcr @19:07:23.25 Building PCR Slug from ASV with MM3 mix, PP5 primer @19:07:23.26 Priming with carrier @19:07:23.27 ATV.dial ARV @19:07:24.29 ARV.dial :carrier @19:07:24.57 AS.seek 0.500ml @19:07:50.49 AFV.select :carrierSolution @19:07:50.59 delay 2 seconds @19:07:52.60 AS.reconfigure ASconfig @19:07:52.64 AS.seek 1.000ml @19:08:22.10 delay 1 second @19:08:23.11 AFV.select :holdingCoil @19:08:23.20 delay 1 second @19:08:24.21 ATV.dial :waste @19:08:25.47 AS.reconfigure ASnorm @19:08:25.50 AS.seek empty @19:09:16.97 AFV.select :carrierSolution @19:09:17.07 delay 2 seconds @19:09:19.08 AS.reconfigure ASconfig @19:09:19.11 AS.seek 0.500ml @19:09:48.60 delay 1 second @19:09:49.61 AFV.select :holdingCoil @19:09:49.70 Building Slug @19:09:49.71 ATV.dial :ARV @19:09:51.04 delay 2 seconds @19:09:53.05 ARV.dial :air1 @19:09:53.38 delay 2 seconds @19:09:55.44 AS.reconfigure ASslow2 @19:09:55.47 AS.seek 0.506ml @19:10:01.80 delay 2 seconds @19:10:03.81 ARV.dial :MM3 @19:10:04.68 delay 2 seconds @19:10:06.69 Adding MM3 @19:10:06.76 AS.seek 0.524ml @19:10:25.28 delay 25 seconds @19:10:50.29 ARV.dial :PP5 @19:10:50.71 Adding PP5 @19:10:50.73 delay 2 seconds @19:10:52.78 AS.seek 0.530ml @19:10:59.20 delay 10 seconds @19:11:09.21 ARV.dial :air3 @19:11:09.53 adding ARV air @19:11:09.54 delay 2 seconds @19:11:11.60 AS.seek 0.536ml @19:11:17.95 delay 5 seconds @19:11:22.96 ARV.dial :carrier @19:11:23.99 delay 7 seconds @19:11:31.05 AS.seek 0.594ml @19:12:30.61 delay 5 seconds @19:12:35.62 ATV.dial :ASV @19:12:35.95 delay 2 seconds @19:12:38.01 AS.seek 0.600ml @19:12:44.45 delay 4 seconds @19:12:48.46 ATV.dial ARV @19:12:48.78 delay 2 seconds @19:12:50.79 ARV.dial :carrier @19:12:50.80 delay 2 seconds @19:12:52.85 AS.seek 0.650ml @19:13:44.19 delay 4 seconds @19:13:48.20 ATV.dial PCR @19:13:48.69 ASV.dial :waste1 @19:13:48.70 ARV.dial :air1 @19:13:49.05 Positioning Slug by 0.324ml @19:13:49.10 AS.seek 0.326ml @19:19:19.57 delay 5 seconds @19:19:24.58 ATV.dial ARV @19:19:25.09 ASV.dial :PCRlock @19:19:25.68 delay 5 seconds @19:19:30.69 Running IPCem ProfileEM59C thermocycling @19:19:30.70 PCR.program ProfileEM59C @19:19:30.81 ProfileEM59C should complete in < 2:11:38 @19:19:30.82 PCR.run 2:49:32.5 @21:16:07.63 PCR.off @21:16:08.17 PCR for IPCem completed -- closed /var/log/esp/IPCem14mar1719h400ml.pcr @21:16:08.18 Full MFB Decontamination @21:16:08.19 Decon extraction column and decon AIV @21:16:08.20 SPE.hold 2:46:40,55.0C @21:16:08.22 ASV.dial :SPEdelivery @21:16:08.74 delay 20 seconds @21:16:28.75 AFV.select :carrierSolution @21:16:28.84 delay 2 seconds @21:16:30.85 AS.reconfigure ASconfig @21:16:30.89 AS.seek 0.500ml @21:16:41.34 delay 1 second @21:16:42.35 AFV.select :holdingCoil @21:16:42.44 ATV.dial :air1 @21:16:43.95 AS.reconfigure ASslow1 @21:16:43.98 AS.seek 0.520ml @21:16:48.31 ATV.dial :ASV @21:16:49.59 ASV.dial :SPErecovery @21:16:50.14 AS.seek 0.620ml @21:17:10.79 ATV.dial :waste @21:17:12.25 AS.reconfigure ASnorm @21:17:12.28 AS.seek empty @21:17:44.35 AFV.select :holdingCoil @21:17:44.36 ATV.dial :waste @21:17:44.37 AS.reconfigure ASconfig @21:17:44.40 AS.seek 0.000ml @21:17:44.68 AFV.select :carrierSolution @21:17:44.77 delay 2 seconds @21:17:46.78 AS.seek 0.500ml @21:18:16.19 delay 1 second @21:18:17.20 AFV.select :holdingCoil @21:18:17.29 ATV.dial :air3 @21:18:17.85 AS.reconfigure ASslow1 @21:18:17.88 AS.seek 0.520ml @21:18:22.21 ATV.dial :air3 @21:18:22.27 AS.seek 0.530ml @21:18:24.55 delay 2 seconds @21:18:26.56 ATV.dial :bleach @21:18:26.93 AS.seek 0.710ml @21:19:03.88 delay 3 seconds @21:19:06.90 ATV.dial :air3 @21:19:07.24 delay 3 seconds @21:19:10.30 AS.seek 0.735ml @21:19:15.63 delay 3 seconds @21:19:18.64 ATV.dial :bleach @21:19:19.04 AS.seek 0.720ml @21:19:22.32 delay 3 seconds @21:19:25.33 ATV.dial ASV @21:19:26.21 ASV.dial :SPErecovery @21:19:26.27 AS.seek 0.365ml @21:20:38.89 ATV.dial :waste @21:20:40.36 AS.reconfigure ASnorm @21:20:40.39 AS.seek empty @21:20:59.41 ATV.dial :air3 @21:20:59.97 AS.reconfigure ASslow1 @21:21:00.00 AS.seek 0.009ml @21:21:02.38 delay 2 seconds @21:21:04.39 ATV.dial :bleach @21:21:04.77 AS.seek 0.579ml @21:23:01.31 delay 3 seconds @21:23:04.32 ATV.dial :air3 @21:23:04.67 delay 3 seconds @21:23:07.73 AS.seek 0.604ml @21:23:13.04 delay 3 seconds @21:23:16.05 ATV.dial :bleach @21:23:16.44 AS.seek 0.589ml @21:23:19.73 delay 3 seconds @21:23:22.74 ATV.dial :ASV @21:23:23.72 ASV.dial :wasteR @21:23:24.49 AS.seek 0.000ml @21:25:24.93 delay 1:00 @21:26:24.94 ATV.dial ASV @21:26:24.95 AFV.select :carrierSolution @21:26:25.04 delay 2 seconds @21:26:27.05 AS.reconfigure ASconfig @21:26:27.15 AS.seek 1.000ml @21:27:25.75 delay 1 second @21:27:26.76 AFV.select :holdingCoil @21:27:26.85 delay 1 second @21:27:27.86 ASV.dial :wasteR @21:27:27.87 AS.reconfigure ASslow1 @21:27:27.90 AS.seek 0.000ml @21:30:52.16 delay 10 seconds @21:31:02.17 ATV.dial :air3 @21:31:03.29 AS.seek 0.010ml @21:31:05.60 delay 2 seconds @21:31:07.61 ATV.dial :bleach @21:31:08.01 AS.seek 0.580ml @21:33:04.49 delay 3 seconds @21:33:07.50 ATV.dial :air3 @21:33:07.85 delay 3 seconds @21:33:10.91 AS.seek 0.605ml @21:33:16.25 delay 3 seconds @21:33:19.26 ATV.dial :bleach @21:33:19.65 AS.seek 0.590ml @21:33:22.94 delay 3 seconds @21:33:25.95 ATV.dial ASV @21:33:26.84 ASV.dial :SPEdelivery @21:33:27.88 AS.seek 0.000ml @21:35:28.48 delay 1:00 @21:36:28.49 AFV.select :carrierSolution @21:36:28.58 delay 2 seconds @21:36:30.59 AS.reconfigure ASconfig @21:36:30.63 AS.seek 1.000ml @21:37:29.23 delay 1 second @21:37:30.24 AFV.select :holdingCoil @21:37:30.33 delay 1 second @21:37:31.34 ATV.dial ASV @21:37:31.35 ASV.dial :SPEdelivery @21:37:31.36 AS.reconfigure ASslow1 @21:37:31.39 AS.seek 0.000ml @21:40:55.63 delay 10 seconds @21:41:05.64 ATV.dial :air2 @21:41:06.36 AS.reconfigure ASslow0 @21:41:06.39 AS.seek 0.800ml @21:42:28.30 delay 3 seconds @21:42:31.31 ATV.dial :ASV @21:42:32.13 ASV.dial :SPEdelivery @21:42:32.15 AS.reconfigure ASslow1 @21:42:32.18 AS.seek 0.030ml @21:45:09.48 delay 20 seconds @21:45:29.49 ATV.dial :waste,via => air3 @21:45:30.94 AS.reconfigure ASconfig @21:45:30.97 AS.seek empty @21:45:33.07 SPE.coast @21:45:33.15 Removing Bleach from Mixing Coil @21:45:33.16 AFV.select :carrierSolution @21:45:33.25 delay 2 seconds @21:45:35.26 AS.seek 0.500ml @21:46:04.76 delay 1 second @21:46:05.77 AFV.select :holdingCoil @21:46:05.86 ATV.dial :ASV @21:46:07.42 ASV.dial :SPErecovery @21:46:08.12 AS.reconfigure ASslow1 @21:46:08.15 AS.seek 0.730ml @21:46:55.33 ASV.dial :wasteD @21:46:55.84 AS.reconfigure ASnorm @21:46:55.87 AS.seek 0.000ml @21:47:33.48 AFV.select :carrierSolution @21:47:33.58 delay 2 seconds @21:47:35.59 AS.reconfigure ASconfig @21:47:35.62 AS.seek 0.500ml @21:48:05.08 delay 1 second @21:48:06.09 AFV.select :holdingCoil @21:48:06.18 ATV.dial ASV @21:48:06.19 ASV.dial :SPErecovery @21:48:06.89 AS.reconfigure ASslow0 @21:48:06.93 AS.seek 0.300ml @21:48:27.64 ATV.dial :air2 @21:48:28.48 AS.reconfigure ASslow1 @21:48:28.52 AS.seek 0.320ml @21:48:32.89 ATV.dial ASV @21:48:33.74 AS.reconfigure ASslow0 @21:48:33.77 AS.seek 0.570ml @21:48:59.59 ASV.dial :wasteD @21:49:00.26 AS.reconfigure ASnorm @21:49:00.29 AS.seek 0.000ml @21:49:29.76 AFV.select :carrierSolution @21:49:29.86 delay 2 seconds @21:49:31.87 AS.reconfigure ASconfig @21:49:31.91 AS.seek 0.500ml @21:50:01.36 delay 1 second @21:50:02.37 AFV.select :holdingCoil @21:50:02.46 ATV.dial ASV @21:50:02.47 ASV.dial :SPErecovery @21:50:03.16 AS.reconfigure ASslow0 @21:50:03.20 AS.seek 0.300ml @21:50:23.87 ATV.dial :air2 @21:50:24.61 AS.reconfigure ASslow1 @21:50:24.65 AS.seek 0.320ml @21:50:28.99 ATV.dial ASV @21:50:29.85 AS.reconfigure ASslow0 @21:50:29.89 AS.seek 0.570ml @21:50:55.71 ASV.dial :wasteD @21:50:56.64 AS.reconfigure ASnorm @21:50:56.68 AS.seek 0.000ml @21:51:26.15 ATV.dial :air2 @21:51:26.96 AS.reconfigure ASslow0 @21:51:26.99 AS.seek 0.300ml @21:51:57.87 ATV.dial :ASV @21:51:58.57 ASV.dial :wasteD @21:51:58.59 AS.reconfigure ASconfig @21:51:58.62 AS.seek 0.050ml @21:52:13.48 ATV.dial :waste @21:52:15.00 AS.seek 0.000ml @21:52:18.19 ASV.dial :SPEdelivery @21:52:19.04 deconLysateINT @21:52:19.05 ATV.dial :air3 @21:52:19.62 AS.reconfigure ASslow1 @21:52:19.65 AS.seek 0.010ml @21:52:22.01 delay 2 seconds @21:52:24.02 ATV.dial :bleach @21:52:24.39 AS.seek 0.960ml @21:55:38.39 delay 3 seconds @21:55:41.40 ATV.dial :air3 @21:55:41.81 delay 3 seconds @21:55:44.87 AS.seek 0.985ml @21:55:50.19 delay 3 seconds @21:55:53.20 ATV.dial :bleach @21:55:53.58 AS.seek 0.970ml @21:55:56.89 delay 3 seconds @21:55:59.90 ATV.dial :AIV @21:56:01.08 AIV.dial :coilToMFB @21:56:01.09 AWV.select :waste @21:56:01.10 AFV.select :carrierSolution @21:56:01.19 delay 2 seconds @21:56:03.20 AS.reconfigure ASconfig @21:56:03.23 AS.seek 1.000ml @21:56:05.31 delay 1 second @21:56:06.32 AFV.select :holdingCoil @21:56:06.41 delay 1 second @21:56:07.42 AS.reconfigure ASslow0 @21:56:07.45 AS.seek empty @21:57:49.84 AFV.select :carrierSolution @21:57:49.94 delay 2 seconds @21:57:51.95 AS.reconfigure ASconfig @21:57:51.98 AS.seek 1.000ml @21:58:50.63 delay 1 second @21:58:51.64 AFV.select :holdingCoil @21:58:51.73 delay 1 second @21:58:52.74 AS.reconfigure ASslow0 @21:58:52.77 AS.seek empty @22:00:35.18 AFV.select :carrierSolution @22:00:35.27 delay 2 seconds @22:00:37.28 AS.reconfigure ASconfig @22:00:37.86 AS.seek 1.000ml @22:01:36.54 delay 1 second @22:01:37.55 AFV.select :holdingCoil @22:01:37.64 delay 1 second @22:01:38.65 AS.reconfigure ASslow0 @22:01:38.68 AS.seek empty @22:03:21.06 ATV.dial :air2 @22:03:21.65 AS.reconfigure ASslow1 @22:03:21.68 AS.seek 0.009ml @22:03:24.04 AS.reconfigure ASslow0 @22:03:24.07 AS.seek 1.000ml @22:05:05.41 ATV.dial AIV @22:05:06.03 AS.seek 0.020ml @22:06:46.32 ATV.dial :waste @22:06:47.59 AS.seek 0.000ml @22:06:49.93 AWV.select :air @22:06:50.08 Cleaning PCR @22:06:50.09 AFV.select :carrierSolution @22:06:50.19 delay 2 seconds @22:06:52.20 AS.reconfigure ASconfig @22:06:52.23 AS.seek 0.500ml @22:07:21.71 delay 1 second @22:07:22.72 AFV.select :holdingCoil @22:07:22.81 ASV.dial :waste1 @22:07:23.14 ATV.dial :air3 @22:07:23.71 AS.reconfigure ASslow1 @22:07:23.74 AS.seek 0.510ml @22:07:26.02 delay 2 seconds @22:07:28.03 ATV.dial :bleach @22:07:28.41 AS.seek 0.810ml @22:08:29.86 delay 3 seconds @22:08:32.87 ATV.dial :air3 @22:08:33.22 delay 3 seconds @22:08:36.28 AS.seek 0.835ml @22:08:41.67 delay 3 seconds @22:08:44.69 ATV.dial :bleach @22:08:45.08 AS.seek 0.820ml @22:08:48.36 delay 3 seconds @22:08:51.37 ARV.dial :waste @22:08:51.88 delay 3 seconds @22:08:54.89 ATV.dial :ARV @22:08:55.74 AS.reconfigure ASconfig @22:08:55.77 AS.seek 0.470ml @22:09:16.45 AFV.select :carrierSolution @22:09:16.54 delay 2 seconds @22:09:18.55 AS.seek 1.000ml @22:09:49.73 delay 1 second @22:09:50.74 AFV.select :holdingCoil @22:09:50.83 delay 1 second @22:09:51.84 AS.reconfigure ASfast @22:09:51.88 AS.seek 0.000ml @22:10:19.61 AFV.select :carrierSolution @22:10:19.71 delay 2 seconds @22:10:21.72 AS.reconfigure ASconfig @22:10:21.75 AS.seek 1.000ml @22:11:20.37 delay 1 second @22:11:21.38 AFV.select :holdingCoil @22:11:21.47 delay 1 second @22:11:22.48 AS.reconfigure ASfast @22:11:22.52 AS.seek 0.000ml @22:11:50.28 AFV.select :carrierSolution @22:11:50.38 delay 2 seconds @22:11:52.39 AS.reconfigure ASconfig @22:11:52.42 AS.seek 1.000ml @22:12:51.02 delay 1 second @22:12:52.03 AFV.select :holdingCoil @22:12:52.23 delay 1 second @22:12:53.24 AS.reconfigure ASfast @22:12:53.27 AS.seek 0.000ml @22:13:20.98 ARV.dial between carrier and air1 @22:13:21.40 ATV.dial :air3 @22:13:22.34 AS.reconfigure ASslow1 @22:13:22.37 AS.seek 0.010ml @22:13:24.73 delay 2 seconds @22:13:26.74 ATV.dial :bleach @22:13:27.12 AS.seek 0.810ml @22:16:10.56 delay 3 seconds @22:16:13.57 ATV.dial :air3 @22:16:13.89 delay 3 seconds @22:16:16.95 AS.seek 0.835ml @22:16:22.31 delay 3 seconds @22:16:25.32 ATV.dial :bleach @22:16:25.80 AS.seek 0.820ml @22:16:29.25 delay 3 seconds @22:16:32.26 ATV.dial PCR @22:16:32.70 AS.reconfigure ASconfig @22:16:32.73 AS.seek empty @22:17:20.87 PCR.program :bleachProfile @22:17:20.92 PCR program should complete in < 2:45 @22:17:20.93 PCR.run 10:00 @22:20:25.16 PCR.off @22:20:25.19 AFV.select :carrierSolution @22:20:25.28 delay 2 seconds @22:20:27.29 AS.seek 1.000ml @22:21:25.94 delay 1 second @22:21:26.95 AFV.select :holdingCoil @22:21:27.04 delay 1 second @22:21:28.05 AS.reconfigure ASfast @22:21:28.08 AS.seek 0.000ml @22:21:55.81 delay 3 seconds @22:21:58.82 AFV.select :carrierSolution @22:21:58.91 delay 2 seconds @22:22:00.92 AS.reconfigure ASconfig @22:22:00.95 AS.seek 1.000ml @22:22:59.56 delay 1 second @22:23:00.57 AFV.select :holdingCoil @22:23:00.66 delay 1 second @22:23:01.67 AS.reconfigure ASfast @22:23:01.71 AS.seek 0.000ml @22:23:29.44 delay 3 seconds @22:23:32.45 AFV.select :carrierSolution @22:23:32.55 delay 2 seconds @22:23:34.56 AS.reconfigure ASconfig @22:23:34.59 AS.seek 1.000ml @22:24:33.18 delay 1 second @22:24:34.19 AFV.select :holdingCoil @22:24:34.28 delay 1 second @22:24:35.29 AS.reconfigure ASfast @22:24:35.32 AS.seek 0.000ml @22:25:03.04 delay 3 seconds @22:25:06.05 AFV.select :carrierSolution @22:25:06.14 delay 2 seconds @22:25:08.66 AS.reconfigure ASconfig @22:25:08.69 AS.seek 1.000ml @22:26:07.31 delay 1 second @22:26:08.32 AFV.select :holdingCoil @22:26:08.41 delay 1 second @22:26:09.42 AS.reconfigure ASfast @22:26:09.45 AS.seek 0.000ml @22:26:37.19 delay 3 seconds @22:26:40.20 AFV.select :carrierSolution @22:26:40.29 delay 2 seconds @22:26:42.30 AS.reconfigure ASconfig @22:26:42.34 AS.seek 1.000ml @22:27:40.96 delay 1 second @22:27:41.97 AFV.select :holdingCoil @22:27:42.09 delay 1 second @22:27:43.10 AS.reconfigure ASnorm @22:27:43.14 AS.seek 0.000ml @22:28:34.59 AFV.select :carrierSolution @22:28:34.68 delay 2 seconds @22:28:36.69 AS.reconfigure ASconfig @22:28:36.73 AS.seek 1.000ml @22:29:35.35 delay 1 second @22:29:36.36 AFV.select :holdingCoil @22:29:36.45 delay 1 second @22:29:37.46 ATV.dial :waste @22:29:38.34 AS.reconfigure ASnorm @22:29:38.37 AS.seek 0.500ml @22:30:04.30 ATV.dial :waste2 @22:30:05.50 AS.seek 0.000ml @22:30:31.41
Cleaning PCR AFV.select :carrierSolution @22:30:31.51 delay 2 seconds @22:30:33.52 AS.reconfigure ASconfig @22:30:33.55 AS.seek 0.500ml @22:31:02.98 delay 1 second @22:31:03.99 AFV.select :holdingCoil @22:31:04.08 ASV.dial :waste1 @22:31:04.09 ATV.dial :air3 @22:31:05.58 AS.reconfigure ASslow1 @22:31:05.61 AS.seek 0.510ml @22:31:07.94 delay 2 seconds @22:31:09.95 ATV.dial :bleach @22:31:10.33 AS.seek 0.810ml @22:32:11.79 delay 3 seconds @22:32:14.80 ATV.dial :air3 @22:32:15.14 delay 3 seconds @22:32:18.20 AS.seek 0.835ml @22:32:23.56 delay 3 seconds @22:32:26.58 ATV.dial :bleach @22:32:26.97 AS.seek 0.820ml @22:32:30.26 delay 3 seconds @22:32:33.27 ARV.dial :waste @22:32:33.67 delay 3 seconds @22:32:36.68 ATV.dial :ARV @22:32:37.53 AS.reconfigure ASconfig @22:32:37.56 AS.seek 0.470ml @22:32:58.25 AFV.select :carrierSolution @22:32:58.34 delay 2 seconds @22:33:00.35 AS.seek 1.000ml @22:33:31.57 delay 1 second @22:33:32.59 AFV.select :holdingCoil @22:33:32.68 delay 1 second @22:33:33.69 AS.reconfigure ASfast @22:33:33.72 AS.seek 0.000ml @22:34:01.45 AFV.select :carrierSolution @22:34:01.54 delay 2 seconds @22:34:03.55 AS.reconfigure ASconfig @22:34:03.58 AS.seek 1.000ml @22:35:02.16 delay 1 second @22:35:03.17 AFV.select :holdingCoil @22:35:03.26 delay 1 second @22:35:04.27 AS.reconfigure ASfast @22:35:04.31 AS.seek 0.000ml @22:35:32.04 AFV.select :carrierSolution @22:35:32.13 delay 2 seconds @22:35:34.14 AS.reconfigure ASconfig @22:35:34.17 AS.seek 1.000ml @22:36:32.79 delay 1 second @22:36:33.80 AFV.select :holdingCoil @22:36:33.89 delay 1 second @22:36:34.90 AS.reconfigure ASfast @22:36:34.93 AS.seek 0.000ml @22:37:02.63 ARV.dial between carrier and air1 @22:37:03.03 ATV.dial :air3 @22:37:03.96 AS.reconfigure ASslow1 @22:37:03.99 AS.seek 0.010ml @22:37:06.32 delay 2 seconds @22:37:08.33 ATV.dial :bleach @22:37:08.71 AS.seek 0.810ml @22:39:52.12 delay 3 seconds @22:39:55.13 ATV.dial :air3 @22:39:55.46 delay 3 seconds @22:39:58.52 AS.seek 0.835ml @22:40:03.85 delay 3 seconds @22:40:06.86 ATV.dial :bleach @22:40:07.24 AS.seek 0.820ml @22:40:10.55 delay 3 seconds @22:40:13.56 ATV.dial PCR @22:40:13.88 AS.reconfigure ASconfig @22:40:13.91 AS.seek empty @22:41:02.04 PCR.program :bleachProfile @22:41:02.10 PCR program should complete in < 2:45 @22:41:02.11 PCR.run 10:00 @22:44:06.34 PCR.off @22:44:06.37 AFV.select :carrierSolution @22:44:06.46 delay 2 seconds @22:44:08.47 AS.seek 1.000ml @22:45:07.14 delay 1 second @22:45:08.16 AFV.select :holdingCoil @22:45:08.25 delay 1 second @22:45:09.26 AS.reconfigure ASfast @22:45:09.29 AS.seek 0.000ml @22:45:37.04 delay 3 seconds @22:45:40.05 AFV.select :carrierSolution @22:45:40.14 delay 2 seconds @22:45:42.15 AS.reconfigure ASconfig @22:45:42.18 AS.seek 1.000ml @22:46:40.78 delay 1 second @22:46:41.79 AFV.select :holdingCoil @22:46:41.88 delay 1 second @22:46:42.89 AS.reconfigure ASfast @22:46:42.92 AS.seek 0.000ml @22:47:10.65 delay 3 seconds @22:47:13.66 AFV.select :carrierSolution @22:47:14.22 delay 2 seconds @22:47:16.23 AS.reconfigure ASconfig @22:47:16.26 AS.seek 1.000ml @22:48:14.85 delay 1 second @22:48:15.87 AFV.select :holdingCoil @22:48:15.96 delay 1 second @22:48:16.97 AS.reconfigure ASfast @22:48:17.01 AS.seek 0.000ml @22:48:44.72 delay 3 seconds @22:48:47.73 AFV.select :carrierSolution @22:48:47.82 delay 2 seconds @22:48:49.83 AS.reconfigure ASconfig @22:48:49.86 AS.seek 1.000ml @22:49:48.46 delay 1 second @22:49:49.47 AFV.select :holdingCoil @22:49:49.56 delay 1 second @22:49:50.57 AS.reconfigure ASfast @22:49:50.60 AS.seek 0.000ml @22:50:18.33 delay 3 seconds @22:50:21.34 AFV.select :carrierSolution @22:50:21.43 delay 2 seconds @22:50:23.44 AS.reconfigure ASconfig @22:50:23.48 AS.seek 1.000ml @22:51:22.09 delay 1 second @22:51:23.11 AFV.select :holdingCoil @22:51:23.20 delay 1 second @22:51:24.21 AS.reconfigure ASnorm @22:51:24.25 AS.seek 0.000ml @22:52:15.69 Completed {wqPCR} begun 08:00:32 @22:52:15.72 Asleep til 08:00PDT18-Mar-14 @22:52:15.73 AS.stop @22:52:15.75
Sleeping until 08:00PDT18-Mar-14 @22:52:15.77 GoodNews.email "Then, in tube 3: wqPCR\n/home/esp/esp2/mission/14mar14sccwrp.rb:68",Subject => "Asleep until 08:00PDT18-Mar-14" @22:52:17.41 CC.stop @22:52:17.43 CS.stop @22:52:17.46 PC.stop @22:52:17.49 PS.stop @22:52:17.52 Forearm.stop @22:52:17.55 Elbow.stop @22:52:17.58 SS.stop @22:52:17.66 Sleepy.power :raw,:OFF @22:52:17.67 Sleepy.power :dwarf,:OFF Shutdown @08:00:33.08PDT18-Mar-14
Powering up Dwarves @08:00:37.95 Sleepy.power :raw,:ON @08:00:38.42 SPE.configure SPEconfig @08:00:38.44 AS.configure ASconfig @08:00:38.47 CC.configure CCconfig @08:00:38.50 CS.configure CSconfig @08:00:38.53 PC.configure PCconfig @08:00:38.56 PS.configure PSconfig @08:00:38.59 Forearm.configure LooseForearm @08:00:38.62 Elbow.configure LooseElbow @08:00:38.65 SC.configure SCloose @08:00:38.68 SE.configure SEconfig @08:00:38.71 SS.configure SSconfig @08:00:38.74 CH.configure CHconfig @08:00:38.76 PH.configure PHconfig @08:00:38.79 Valve.reset @08:00:39.45 PCR.off @08:00:39.48 PCR.pidtemp= 50,0.1,0,300,25,25 @08:00:39.51 PCR.caltemp= 6.6661,0.0344,0.0 @08:00:39.61 Intake.select :close @08:00:39.64 Exhaust.select :close CTV.dial :air @08:00:39.68 PSV.dial :air @08:00:39.71
PC.reconfigure PCconfig @08:00:39.77 PC.home.seek open @08:00:39.79 CSV.dial :waste1 @08:00:40.16 PS.reconfigure PSconfig @08:00:40.20 PS.home.seek 0.000ml @08:00:40.32 SSV.dial :spit,avoiding => exhaust @08:00:41.11 CS.reconfigure CSfast @08:00:41.14 SS.reconfigure SSconfig @08:00:41.23 SS.home.seek 0.00ml @08:00:41.25 CS.home.seek 0.000ml @08:00:41.33 CC.reconfigure CCconfig @08:00:41.36 CC.home.seek open @08:00:50.44 PS.coast @08:00:50.52
CC.seek open @08:00:50.54 PC.seek open @08:00:51.13
Forearm.reconfigure LooseForearm @08:00:51.17 Forearm.home.seek retract @08:00:51.92 Hand.grip @08:00:51.95
Elbow.jog -1500 @08:00:52.45 Elbow.reconfigure LooseElbow @08:00:52.48 Elbow.home.seek clear @08:00:57.20 SE.reconfigure SEconfig @08:00:57.23 SE.home.seek down @08:00:57.77 SC.reconfigure SCsloppy @08:00:57.81 SC.home.seek initial @08:01:01.58 Forearm.seek retract @08:01:01.64 Elbow.seek hammer @08:01:02.83 Forearm.reconfigure TightForearm @08:01:02.88 Forearm.seek 2 @08:01:03.88 Forearm.seek retract @08:01:04.96 Forearm.reconfigure LooseForearm @08:01:05.35PDT18-Mar-14 ===> STARTED wqPCR (phase #14) <=== @08:01:05.36 Starting initialPurge @08:01:05.37 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:01:05.42 Hand.release @08:01:06.29 Elbow.reconfigure TightElbow @08:01:06.32 Elbow.seek FlushPuck::Garage @08:01:08.10 Elbow.reconfigure LooseElbow @08:01:08.18 Forearm.seek garage @08:01:09.20 Hand.grip @08:01:10.04 Forearm.seek retract @08:01:11.06 Elbow.reconfigure TightElbow @08:01:11.10 Elbow.seek CC @08:01:13.85 Elbow.reconfigure LooseElbow @08:01:13.94 Forearm.reconfigure TightForearm @08:01:13.97 Forearm.seek CC @08:01:15.04 CC.seek closed @08:01:18.79 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.94 @08:01:18.85 Hand.release @08:01:19.63 Forearm.reconfigure LooseForearm @08:01:19.66 Forearm.seek retract @08:01:20.61 Flushing the Collection Valve::Manifold @08:01:20.70 CS Pulling 5ml of flush @08:01:20.71 CSV.dial CSR @08:01:21.97 CSR.select :flush @08:01:22.07 CS.reconfigure CSconfig @08:01:22.11 CS.seek 5.000ml @08:01:35.54 delay 5 seconds @08:01:40.55 CSR.select :air @08:01:40.69 CS.reconfigure CSfast @08:01:40.72 CS.seek 7.499ml @08:01:47.35 CSV.dial :waste @08:01:48.60 CS.reconfigure CSconfig @08:01:48.64 CS.seek 0.000ml @08:02:08.35 Purging external Intake @08:02:08.44 CSV.dial :flush @08:02:08.89 CS.reconfigure CSfast @08:02:08.92 CS.seek 10.000ml @08:02:32.78 delay 5 seconds @08:02:37.79 CSV.dial CBV @08:02:37.81
CBV.dial CSV @08:02:38.60 CTV.dial :intake @08:02:39.35 Intake.select :open @08:02:39.44 CS.seek 0.000ml @08:03:03.53 Intake.select :close @08:03:03.62 CTV.dial :air @08:03:04.38 Flushing upper manifold @08:03:04.47 CSV.dial :flush @08:03:05.26 CS.seek 5.000ml @08:03:17.62 delay 5 seconds @08:03:22.63 CSV.dial :air @08:03:23.10 CS.seek 7.000ml @08:03:28.62 CSV.dial CBV @08:03:28.64
CBV.dial CSV @08:03:29.70 CTV.dial :air @08:03:29.72 CS.seek 0.000ml @08:03:46.65 delay 7 seconds @08:03:53.67 CS.seek 3.000ml @08:04:01.50 CSV.dial :waste @08:04:02.01 CS.seek 0.000ml @08:04:10.05 CSV.dial :flush @08:04:10.49 CS.seek 3.000ml @08:04:18.22 delay 5 seconds @08:04:23.23 CSV.dial :air @08:04:23.70 CS.seek 5.000ml @08:04:29.12 CSV.dial :air @08:04:29.14 CS.seek 0.000ml @08:04:41.50 delay 7 seconds @08:04:48.52 CS.seek 3.000ml @08:04:56.37 CSV.dial :waste @08:04:57.16 CS.seek 0.000ml @08:05:05.06 Garaging FlushPuck in Collection Clamp @08:05:05.12 Elbow.reconfigure TightElbow @08:05:05.15 Elbow.seek CC @08:05:05.35 Elbow.reconfigure LooseElbow @08:05:05.43 Forearm.seek CC @08:05:06.39 CC.seek sealed @08:05:07.34 Hand.grip @08:05:08.08 CC.seek open @08:05:11.40 Forearm.seek retract @08:05:12.90 Elbow.reconfigure TightElbow @08:05:12.93 Elbow.seek garage @08:05:15.62 Elbow.reconfigure LooseElbow @08:05:15.71 Forearm.reconfigure TightForearm @08:05:15.74 Forearm.seek garage @08:05:16.85 Hand.release @08:05:17.62 Forearm.reconfigure LooseForearm @08:05:17.65 Forearm.seek retract @08:05:18.84 Starting mfb @08:05:18.85 pcrBAC sampling at most 1000ml, wcr at most 1000ml @08:05:18.86 BACfans.email "Can@08:01:05, 16.2C, 69% humidity, 14.3psia, 13.739V, 0.567A, 0.567A avg, 7.79W\nCTD data unavailable\nISUS data unavailable",Subject => "pcrBAC sampling at most 1000ml, wcr at most 1000ml" @08:05:20.49
Loading sh1 puck from tube 3 into Collection Clamp @08:05:20.58 Readying the MFB @08:05:20.59 Homing MFB Syringe @08:05:20.60 AFV.select :holdingCoil @08:05:20.78
SC.reconfigure SCtight @08:05:20.84 SC.seek 3 @08:05:20.89 ATV.dial :air2 @08:05:22.18 AS.reconfigure ASconfig @08:05:22.22 AS.home.seek home @08:05:22.99
SC.reconfigure SCloose @08:05:23.17 Elbow.reconfigure TightElbow @08:05:23.20 Elbow.seek 3 @08:05:25.03 Forearm.reconfigure TightForearm @08:05:25.06 Forearm.seek 3 + 800 counts @08:05:26.10 SE.reconfigure SEpick @08:05:26.15 SE.seek beamBreak @08:05:28.67 SE.configure SEconfig @08:05:28.80 Elbow.seek 3 @08:05:28.95 Forearm.seek 3 + 300 counts @08:05:29.10 Forearm.reconfigure LooseForearm @08:05:29.14 Hand.grip @08:05:29.73 AFV.select :carrierSolution @08:05:29.84 SE.seek down @08:05:29.97 delay 2 seconds @08:05:30.01
Forearm.seek retract @08:05:30.95 Elbow.seek CC @08:05:31.98 AS.seek 1.000ml @08:05:32.23 SE.coast @08:05:32.54
Elbow.reconfigure LooseElbow @08:05:32.62 Forearm.reconfigure TightForearm @08:05:32.65 Forearm.seek CC @08:05:33.79 CC.seek closed @08:05:37.54 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.96 @08:05:37.55 Hand.release @08:05:38.37 Forearm.reconfigure LooseForearm @08:05:38.40 Forearm.seek retract @08:05:39.32 SC.coast @08:05:39.35 collectSample 1000ml w/bubble point of 55psi Priming 7ml @08:05:39.36 Sleepy.power :sampler,:ON @08:05:39.37 Intake.select :close @08:05:39.38 Exhaust.select :close @08:05:39.39 SS.hold @08:05:39.54 Calibrating pressure and temperature sensors @08:05:39.55 CTV.dial :air @08:05:39.56 SSV.dial :spit,avoiding => exhaust @08:05:39.58 SS.reconfigure SSfast @08:05:39.61 SS.seek 5.00ml @08:05:42.79 SSV.dial CBV @08:05:42.81
CBV.dial SSV @08:05:44.13 delay 3.8 seconds @08:05:48.01 SSV.dial :spit @08:05:49.34 SS.seek 0.00ml @08:05:51.82 SSV.dial CBV @08:05:53.61 CTV.dial :intake @08:05:54.35 Waiting for external sampler to finish priming... @08:05:54.36 delayUntil "08:15:39.36" @08:06:23.70 delay 1 second @08:06:24.71 AFV.select :holdingCoil @08:06:24.80 delay 1 second @08:06:25.81 ATV.dial :waste @08:06:26.80 AS.reconfigure ASnorm @08:06:26.83 AS.home.seek empty @08:07:18.90 ATV.dial :waste @08:07:18.91 ARV.dial between carrier and air1 @08:07:18.97 ASV.dial :waste1 @08:15:39.36 SSV.dial CBV @08:15:39.38
CBV.dial SSV @08:15:39.39 Allowing 55s for 7.0ml of remaining 7.0ml Intake.select :open @08:15:41.52 Intake @14.6psia, vacuum from 8.1 to 12.1psi @08:15:41.53 SS.reconfigure forSampling @08:15:41.57 SS.seek 7.00ml @08:15:47.02 Stroke completed -- waiting for pressure to equalize ... @08:15:49.42 Intake.select :close @08:15:49.56 SSV.dial :exhaust @08:15:50.32 Exhaust.select :open @08:15:50.42 SS.reconfigure SSexhale @08:15:50.46 SS.seek 0.00ml @08:15:54.30 Exhaust.select :close @08:15:54.40 Sampling 1000.0ml @08:15:54.41 Intake.select :close @08:15:54.42 Exhaust.select :close @08:15:54.43 SS.reconfigure SSconfig @08:15:54.46 SS.hold @08:15:54.62 CTV.dial :intake @08:15:54.63 SSV.dial CBV @08:15:54.65
CBV.dial SSV @08:15:55.41 Allowing 185s for 25.0ml of remaining 1000.0ml Intake.select :open @08:15:57.54 Intake @14.1psia, vacuum from 7.6 to 11.6psi @08:15:57.55 SS.reconfigure forSampling @08:15:57.59 SS.seek 25.00ml @08:15:59.39 Vacuum too high -- waiting up to 40s @08:16:39.59 SS.reconfigure SSconfig @08:16:39.62 SS.hold @08:16:39.70 Intake.select :close @08:16:39.86 ShallowSampler::Clogged in MAIN -- Intake @14.3psia, & Outlet @2.0psia -- no progress after 1.1ml SSV.dial :exhaust @08:16:40.61 Exhaust.select :open @08:16:40.72 SS.reconfigure SSexhale @08:16:40.76 SS.seek 0.00ml @08:16:42.20 Exhaust.select :close @08:16:42.30 Sampled 1.1ml @08:16:42.31 collectSample: Complete @08:16:42.32 Evacuating Puck (bubblePt=55.0psi, delay=15.0s) Intake.select :close @08:16:42.38 CSV.dial :waste @08:16:42.41 CS.seek 0.000ml @08:16:42.50 SSV.dial CBV,avoiding => exhaust @08:16:42.53
CBV.dial SSV @08:16:42.57 CSV.dial :air @08:16:43.36 CS.seek 10.000ml @08:16:44.39
CTV.dial :air @08:16:45.14 delay 15 seconds @08:16:45.29 Sleepy.power :sampler,:OFF @08:17:00.14
SSV.dial :spit,avoiding => exhaust @08:17:01.45 CTV.dial CSV,avoiding => intake @08:17:01.47
SS.seek 0.00ml @08:17:01.56 SSV.dial CBV,avoiding => exhaust @08:17:07.76 CBV.dial between CSV and waste2b @08:17:08.70 CTV.dial CSV,avoiding => air @08:17:08.72
CSV.dial CTV @08:17:09.18 CS.reconfigure CSpressurize @08:17:09.21 CS.seek 0.000ml @08:17:35.13 CSV.dial :air @08:17:35.65 CS.reconfigure CSfast @08:17:35.71 CS.seek 10.000ml @08:17:59.70 CSV.dial between air and CTV @08:18:00.09 CS.seek 3.039ml @08:18:16.73 CTV.dial CSV,avoiding => air @08:18:16.74
CSV.dial CTV @08:18:17.03 CS.reconfigure CSpressurize @08:18:17.06 CS.seek 0.000ml @08:18:21.89 BubblePoint: Intake @60.3psia, Outlet @5.2psia @08:18:21.90 SS.reconfigure SSconfig @08:18:21.93 SS.seek 10.00ml @08:18:27.62 delay 15 seconds @08:18:42.72 Evacuated: Intake @59.4psia, Outlet @-0.2psia @08:18:42.73 SSV.dial :spit,avoiding => exhaust @08:18:44.03 SS.reconfigure SSexhale @08:18:44.06 SS.seek 0.00ml @08:18:48.96 SS.reconfigure SSfast @08:18:48.99 SS.seek 5.00ml @08:18:51.70 SSV.dial CBV,avoiding => exhaust @08:18:51.72
CBV.dial SSV @08:18:53.05 CTV.dial :air,avoiding => intake @08:18:54.34 CSV.dial CTV @08:18:54.36
SS.reconfigure SSconfig @08:18:54.40 SS.seek 10.00ml @08:18:55.36 CSV.dial :air @08:18:55.88 CS.reconfigure CSfast @08:18:55.91 CS.seek 0.000ml @08:18:57.58
SSV.dial :spit,avoiding => exhaust @08:18:58.91 SS.reconfigure SSexhale @08:18:58.94 SS.seek 0.00ml @08:18:59.84 CS.coast @08:19:03.99
SS.reconfigure SSfast @08:19:04.03 SS.seek 5.00ml @08:19:06.86 SSV.dial CBV,avoiding => exhaust @08:19:06.88
CBV.dial SSV @08:19:09.19 SSV.dial :spit,avoiding => exhaust @08:19:10.50 SS.reconfigure SSconfig @08:19:10.53 SS.seek 0.00ml @08:19:13.55 SS.coast @08:19:13.58 Evacuating Puck (bubblePt=55.0psi, delay=15.0s) Intake.select :close @08:19:13.64 CSV.dial :waste @08:19:13.73 SSV.dial CBV,avoiding => exhaust @08:19:13.75
CBV.dial SSV @08:19:14.38 CS.seek 0.000ml @08:19:14.44 CSV.dial :air @08:19:15.58
CTV.dial :air @08:19:15.59 delay 15 seconds @08:19:15.61 CS.seek 10.000ml @08:19:30.59
SSV.dial :spit,avoiding => exhaust @08:19:31.90 CTV.dial CSV,avoiding => intake @08:19:31.92
SS.reconfigure SSexhale @08:19:31.98 SS.seek 0.00ml @08:19:32.04 SSV.dial CBV,avoiding => exhaust @08:19:39.61 CBV.dial between CSV and waste2b @08:19:40.53 CSV.dial between air and CTV @08:19:40.83 CS.seek 8.243ml @08:19:45.69 CTV.dial CSV,avoiding => air @08:19:45.70
CSV.dial CTV @08:19:45.99 CS.reconfigure CSpressurize @08:19:46.02 CS.seek 0.000ml @08:20:07.66 CSV.dial :air @08:20:08.18 CS.reconfigure CSfast @08:20:08.21 CS.seek 10.000ml @08:20:32.00 CSV.dial between air and CTV @08:20:32.38 CS.seek 3.037ml @08:20:49.00 CTV.dial CSV,avoiding => air @08:20:49.01
CSV.dial CTV @08:20:49.30 CS.reconfigure CSpressurize @08:20:49.33 CS.seek 0.000ml @08:20:56.39 BubblePoint: Intake @69.5psia, Outlet @14.3psia @08:20:56.40 SS.reconfigure SSconfig @08:20:56.43 SS.seek 10.00ml @08:21:02.28 delay 15 seconds @08:21:17.38 Evacuated: Intake @68.3psia, Outlet @0.0psia @08:21:17.39 SSV.dial :spit,avoiding => exhaust @08:21:18.70 SS.reconfigure SSexhale @08:21:18.73 SS.seek 0.00ml @08:21:24.05 SS.reconfigure SSfast @08:21:24.09 SS.seek 5.00ml @08:21:26.76 SSV.dial CBV,avoiding => exhaust @08:21:26.78
CBV.dial SSV @08:21:28.12 CTV.dial :air,avoiding => intake @08:21:29.42 CSV.dial CTV @08:21:29.43
SS.reconfigure SSconfig @08:21:29.47 SS.seek 10.00ml @08:21:30.43 CSV.dial :air @08:21:30.94 CS.reconfigure CSfast @08:21:30.97 CS.seek 0.000ml @08:21:32.90
SSV.dial :spit,avoiding => exhaust @08:21:32.99 CS.coast @08:21:34.21
SS.reconfigure SSexhale @08:21:34.24 SS.seek 0.00ml @08:21:39.15 SS.reconfigure SSfast @08:21:39.19 SS.seek 5.00ml @08:21:42.01 SSV.dial CBV,avoiding => exhaust @08:21:42.03
CBV.dial SSV @08:21:44.33 SSV.dial :spit,avoiding => exhaust @08:21:45.63 SS.reconfigure SSconfig @08:21:45.66 SS.seek 0.00ml @08:21:48.75 SS.coast @08:21:48.78 Lysing: Adding 1.5ml lysis CBV.dial between CSV and waste1,avoiding => DBV @08:21:50.25 CTV.dial CSV @08:21:51.49 CSV.dial CSR @08:21:52.27 CSR.select :lysis @08:21:52.37 CS.reconfigure CSconfig @08:21:52.41 CS.seek 1.000ml @08:21:55.74 delay 5 seconds @08:22:00.75 CSR.select :air @08:22:00.89 CS.seek 6.000ml @08:22:14.33 delay 2 seconds @08:22:16.34 CSV.dial :waste1 @08:22:17.59 CS.reconfigure CSfast @08:22:17.62 CS.seek empty @08:22:32.08 CSV.dial CSR @08:22:33.35 CSR.select :lysis @08:22:33.45 CS.reconfigure CSconfig @08:22:33.49 CS.seek 1.500ml @08:22:38.08 delay 5 seconds @08:22:43.09 CSR.select :air @08:22:43.23 CS.seek 4.500ml @08:22:51.58 delay 2 seconds @08:22:53.60 CSV.dial CTV @08:22:54.11 CS.seek 1.275ml @08:23:03.12 Heating sh1 puck from tube 3 in Collection Clamp to 85.0C for 5:00 @08:23:03.13 CH.seek 85.0C,8:00 @08:25:49.33 CH.hold 5:00,85.0C @08:30:49.43 Collection Puck Heater at 85.0C after lysing @08:30:49.44 CS.seek 0.000ml @08:30:53.70 CTV.dial between spare and air,avoiding => intake @08:30:54.69 CSV.dial :air @08:30:55.15 CS.seek 5.000ml @08:31:08.59 CSV.dial CTV @08:31:09.11 delay 2 seconds @08:31:11.12 CSV.dial :air @08:31:11.64 delay 2 seconds @08:31:13.65 CSV.dial CTV @08:31:14.17 delay 2 seconds @08:31:16.18 CSV.dial :air @08:31:16.70 delay 2 seconds @08:31:18.71 CS.seek 0.000ml @08:31:32.22 Recovering Lysate into Collection syringe @08:31:32.23 CSV.dial CBV @08:31:33.22 CBV.dial CSV @08:31:33.58 CS.seek 4.999ml @08:31:47.06 delay 5 seconds @08:31:52.07 CTV.dial :air @08:31:52.55 CS.seek 6.999ml @08:31:58.40 delay 5 seconds @08:32:03.41 CSV.dial :air2 @08:32:04.45 CS.seek 10.000ml @08:32:12.87 CSV.dial between air and CTV,avoiding => CBV @08:32:14.01 Lysing Complete @08:32:14.06 Storing sh1 puck in Collection Clamp to tube 2 @08:32:14.16 SE.seek down @08:32:14.51 SC.reconfigure SCtight @08:32:14.54 SC.seek 2 @08:32:16.78 SC.reconfigure SCloose @08:32:16.96 Elbow.reconfigure TightElbow @08:32:16.99 Elbow.seek 2 @08:32:18.59 Forearm.reconfigure TightForearm @08:32:18.62 Forearm.seek 2 + 800 counts @08:32:19.70 SE.reconfigure SEpick @08:32:19.75 SE.seek beamBreak @08:32:21.33 SE.configure SEconfig @08:32:21.45 Elbow.reconfigure LimpElbow @08:32:21.49 Forearm.reconfigure LooseForearm @08:32:21.53 Forearm.seek 2 @08:32:21.77 Elbow.hold @08:32:21.82 Elbow.reconfigure TightElbow @08:32:21.90 SE.seek 16.02 Pucks below Top Plate @08:32:22.30 Forearm.coast @08:32:22.33 Forearm.seek 2 + 334 counts @08:32:22.61 Forearm.reconfigure TightForearm @08:32:22.64 Forearm.seek 2 + 800 counts @08:32:22.81 SE.seek 17.02 Pucks below Top Plate @08:32:23.35 Forearm.reconfigure LooseForearm @08:32:23.38 Forearm.seek retract @08:32:24.30 Elbow.seek CC @08:32:25.89 Elbow.reconfigure LooseElbow @08:32:25.98 Forearm.seek CC @08:32:26.94 CC.seek sealed @08:32:27.87 Hand.grip @08:32:28.64 CC.seek open @08:32:31.98 Forearm.seek retract @08:32:32.91 Elbow.reconfigure TightElbow @08:32:32.95 Elbow.seek 2 @08:32:34.55 Elbow.reconfigure LimpElbow @08:32:34.59 Forearm.seek 2 @08:32:35.58 Elbow.hold @08:32:35.63 Elbow.reconfigure TightElbow @08:32:35.67 Hand.release @08:32:36.47 Forearm.coast @08:32:36.50 Forearm.seek 2 + 323 counts @08:32:36.73 Forearm.reconfigure TightForearm @08:32:36.76 Forearm.seek 2 + 800 counts @08:32:37.03 SE.seek down @08:32:38.09 SE.coast @08:32:38.18
Forearm.reconfigure LooseForearm @08:32:38.21 Forearm.seek retract @08:32:39.12 SC.coast @08:32:39.15 Tranferring lysate to PRV @08:32:39.16 CSV.dial :air @08:32:39.44 CS.seek 10.000ml @08:32:39.49 CSV.dial between air and CTV @08:32:39.86 AIV.dial :bypass @08:32:39.93 Beginning samplePassC @08:32:39.94 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:32:40.08 Elbow.seek FlushPuck::Garage @08:32:41.85 Elbow.reconfigure LooseElbow @08:32:41.93 Forearm.seek garage @08:32:42.90 Hand.grip @08:32:43.73 Forearm.seek retract @08:32:44.71 Elbow.reconfigure TightElbow @08:32:44.75 Elbow.seek CC @08:32:47.58 Elbow.reconfigure LooseElbow @08:32:47.67 Forearm.reconfigure TightForearm @08:32:47.70 Forearm.seek CC @08:32:49.40 CC.seek closed @08:32:53.17 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 2.01 @08:32:53.23 Hand.release @08:32:54.06 Forearm.reconfigure LooseForearm @08:32:54.09 Forearm.seek retract @08:32:55.05 AWV.select :air @08:32:55.06 AIV.dial :bypass @08:32:55.07 PSV.dial PSR @08:32:56.35 PSR.select :flush @08:32:56.46 PS.seek 1.500ml @08:33:12.56 delay 5 seconds @08:33:17.57 PSV.dial :waste1 @08:33:18.01 PS.seek 0.000ml @08:33:34.03 PSV.dial PSR @08:33:34.47 PSR.select :air @08:33:34.57 PS.seek 2.500ml @08:34:00.76 PRV.dial CBV @08:34:00.78
CBV.dial PRV @08:34:01.55 PSV.dial :mixloop @08:34:02.55 CTV.dial :air,avoiding => intake @08:34:02.56 PTV.dial :PRVmixing @08:34:02.63 PS.seek 0.000ml @08:34:28.94 PSV.dial :air @08:34:29.38 PS.seek 2.500ml @08:34:55.66 PSV.dial :mixloop @08:34:56.11 PS.seek 0.000ml @08:35:22.35 PSV.dial :air @08:35:22.80 PS.seek 2.500ml @08:35:49.04 PSV.dial :mixloop @08:35:49.48 PS.seek 0.000ml @08:36:15.69 Garaging FlushPuck in Collection Clamp @08:36:15.75 Elbow.reconfigure TightElbow @08:36:15.78 Elbow.seek CC @08:36:16.00 Elbow.reconfigure LooseElbow @08:36:16.09 Forearm.seek CC @08:36:17.05 CC.seek sealed @08:36:17.99 Hand.grip @08:36:18.77 CC.seek open @08:36:22.08 Forearm.seek retract @08:36:23.13 Elbow.reconfigure TightElbow @08:36:23.17 Elbow.seek garage @08:36:25.95 Elbow.reconfigure LooseElbow @08:36:26.03 Forearm.reconfigure TightForearm @08:36:26.06 Forearm.seek garage @08:36:27.21 Hand.release @08:36:27.93 Forearm.reconfigure LooseForearm @08:36:27.96 Forearm.seek retract @08:36:29.01 Connecting PS to CS to transfer sample @08:36:29.02 CBV.dial AIV @08:36:29.03 AIV.dial :bypass @08:36:29.04 PSV.dial :mixloop @08:36:29.05 PTV.dial :PRVmixing PRV.dial AIV @08:36:29.06 CTV.dial :air,avoiding => intake @08:36:29.07 Loading lyfil puck from tube 3 into Collection Clamp @08:36:29.22 SE.seek down @08:36:29.57 SC.reconfigure SCtight @08:36:29.60 SC.seek 3 @08:36:31.87 SC.reconfigure SCloose @08:36:32.05 Elbow.reconfigure TightElbow @08:36:32.08 Elbow.seek 3 @08:36:33.91 Forearm.reconfigure TightForearm @08:36:33.94 Forearm.seek 3 + 800 counts @08:36:34.98 SE.reconfigure SEpick @08:36:35.03 SE.seek beamBreak @08:36:37.74 SE.configure SEconfig @08:36:37.86 Elbow.seek 3 @08:36:38.00 Forearm.seek 3 + 300 counts @08:36:38.16 Forearm.reconfigure LooseForearm @08:36:38.20 Hand.grip @08:36:38.89 SE.seek down @08:36:39.04
Forearm.seek retract @08:36:40.00 Elbow.seek CC @08:36:41.43 SE.coast @08:36:42.04
Elbow.reconfigure LooseElbow @08:36:42.14 Forearm.reconfigure TightForearm @08:36:42.17 Forearm.seek CC @08:36:43.34 CC.seek closed @08:36:47.15 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.98 @08:36:47.16 Hand.release @08:36:47.93 Forearm.reconfigure LooseForearm @08:36:47.96 Forearm.seek retract @08:36:48.88 SC.coast @08:36:48.91 CTV.dial CSV,avoiding => intake @08:36:50.21 CSV.dial CTV @08:36:50.52 CS.seek 5.000ml @08:37:04.02 delay 1:20 @08:38:24.03 CS.seek 2.500ml @08:38:31.14 delay 40 seconds @08:39:11.15 CS.seek 0.000ml @08:39:18.49 delay 1:00 @08:40:18.50 PSV.dial :air @08:40:18.94 PS.seek 2.500ml @08:40:45.12 PSV.dial :mixloop @08:40:45.56 delay 3 seconds @08:40:48.57 PSV.dial :air @08:40:49.01 delay 2 seconds @08:40:51.02 PSV.dial :mixloop @08:40:51.46 delay 3 seconds @08:40:54.47 PSV.dial :waste1 @08:40:55.74 PS.seek empty @08:41:21.99 CBV.dial :waste1 @08:41:22.46 delay 5 seconds @08:41:27.47 CBV.dial AIV @08:41:27.92 delay 10 seconds @08:41:37.93 CBV.dial :waste1 @08:41:38.40 delay 10 seconds @08:41:48.41 CBV.dial AIV @08:41:48.85 delay 10 seconds @08:41:58.86 CTV.dial CSV,avoiding => intake @08:41:58.87 CBV.dial between AIV and waste1 @08:41:59.20 CSV.dial :air @08:41:59.72 CS.seek 10.000ml @08:42:25.79 CSV.dial CTV @08:42:26.30 CS.seek 0.000ml @08:42:52.63 CBV.dial :waste1 @08:42:52.93 delay 10 seconds @08:43:02.94 CTV.dial :air,avoiding => intake @08:43:04.29 Storing lyfil puck in Collection Clamp to tube 2 @08:43:04.38 SE.seek down @08:43:04.71 SC.reconfigure SCtight @08:43:04.74 SC.seek 2 @08:43:06.95 SC.reconfigure SCloose @08:43:07.12 Elbow.reconfigure TightElbow @08:43:07.16 Elbow.seek 2 @08:43:08.74 Forearm.reconfigure TightForearm @08:43:08.77 Forearm.seek 2 + 800 counts @08:43:09.87 SE.reconfigure SEpick @08:43:09.92 SE.seek beamBreak @08:43:11.22 SE.configure SEconfig @08:43:11.34 Elbow.reconfigure LimpElbow @08:43:11.37 Forearm.reconfigure LooseForearm @08:43:11.41 Forearm.seek 2 @08:43:11.63 Elbow.hold @08:43:11.68 Elbow.reconfigure TightElbow @08:43:11.76 SE.seek 17.03 Pucks below Top Plate @08:43:12.12 Forearm.coast @08:43:12.15 Forearm.seek 2 + 346 counts @08:43:12.39 Forearm.reconfigure TightForearm @08:43:12.42 Forearm.seek 2 + 800 counts @08:43:12.69 SE.seek 18.03 Pucks below Top Plate @08:43:13.20 Forearm.reconfigure LooseForearm @08:43:13.23 Forearm.seek retract @08:43:14.20 Elbow.seek CC @08:43:15.81 Elbow.reconfigure LooseElbow @08:43:15.90 Forearm.seek CC @08:43:16.83 CC.seek sealed @08:43:17.73 Hand.grip @08:43:18.50 CC.seek open @08:43:21.82 Forearm.seek retract @08:43:22.77 Elbow.reconfigure TightElbow @08:43:22.81 Elbow.seek 2 @08:43:24.39 Elbow.reconfigure LimpElbow @08:43:24.96 Forearm.seek 2 @08:43:25.93 Elbow.hold @08:43:25.99 Elbow.reconfigure TightElbow @08:43:26.03 Hand.release @08:43:26.83 Forearm.coast @08:43:26.86 Forearm.seek 2 + 347 counts @08:43:27.15 Forearm.reconfigure TightForearm @08:43:27.18 Forearm.seek 2 + 800 counts @08:43:27.38 SE.seek down @08:43:28.31 SE.coast @08:43:28.48
Forearm.reconfigure LooseForearm @08:43:28.52 Forearm.seek retract @08:43:29.43 SC.coast @08:43:29.46 PSV.dial :air @08:43:30.51 PS.seek 0.499ml @08:43:36.46 delay 3 seconds @08:43:39.47 PSV.dial :mixloop @08:43:39.91 PRV.dial :air @08:43:40.35 delay 3 seconds @08:43:43.36 PRV.dial CBV @08:43:43.80 CBV.dial AIV @08:43:44.26 PRV.dial between CBV and air @08:43:44.58 PRV.dial :air @08:43:44.93 PS.reconfigure PSslow @08:43:44.96 PS.seek 0.599ml @08:43:47.42 PRV.dial :waste2 @08:43:48.15 PS.reconfigure PSconfig @08:43:48.18 PS.seek empty @08:43:55.11 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:43:55.26 Elbow.seek FlushPuck::Garage @08:43:57.01 Elbow.reconfigure LooseElbow @08:43:57.10 Forearm.seek garage @08:43:58.11 Hand.grip @08:43:58.95 Forearm.seek retract @08:43:59.97 Elbow.reconfigure TightElbow @08:44:00.01 Elbow.seek CC @08:44:02.83 Elbow.reconfigure LooseElbow @08:44:02.91 Forearm.reconfigure TightForearm @08:44:02.94 Forearm.seek CC @08:44:04.09 CC.seek closed @08:44:07.93 Collection Clamp onSpring/offSpring for 3/21 samples, on/off torque 2.09 @08:44:07.98 Hand.release @08:44:08.79 Forearm.reconfigure LooseForearm @08:44:08.82 Forearm.seek retract @08:44:09.82 Clearing PML for modified lysate CTV.dial :air,avoiding => intake @08:44:09.83 PRV.dial :waste2 @08:44:09.84 PTV.dial :PRV,avoiding => puck @08:44:10.57 delay 2 seconds @08:44:12.57 PTV.dial :PRVmixing,avoiding => puck @08:44:13.28 delay 2 seconds @08:44:15.29 PSV.dial :PML @08:44:15.30 delay 2 seconds @08:44:17.30 PRV.dial :air @08:44:18.03 delay 2 seconds @08:44:20.09 PS.seek 1.499ml @08:44:36.14 delay 5 seconds @08:44:41.15 PS.seek 2.500ml @08:44:52.17 PSV.dial :air @08:44:52.62 PS.seek 0.000ml @08:45:18.83 mixing lysate with speDil @08:45:18.84 PSV.dial :PML @08:45:19.28 CBV.dial :PRV @08:45:19.29 PRV.dial :air @08:45:19.30 delay 2 seconds @08:45:21.31 PRV.dial :speDil @08:45:21.75 delay 2 seconds @08:45:23.81 PS.reconfigure PSslow1 @08:45:23.84 PS.seek 0.225ml @08:45:47.14 delay 5 seconds @08:45:52.15 PRV.dial CBV @08:45:52.89 delay 2 seconds @08:45:54.95 PS.seek 0.475ml @08:46:20.70 delay 5 seconds @08:46:25.71 PRV.dial :air @08:46:26.17 delay 2 seconds @08:46:28.18 PS.seek 0.925ml @08:47:14.60 delay 10 seconds @08:47:24.61 PRV.dial CBV @08:47:25.62 CTV.dial :air,avoiding => intake @08:47:25.63 PSV.dial :air @08:47:26.08 PS.reconfigure PSconfig @08:47:26.11 PS.seek 2.500ml @08:47:43.01 delay 3 seconds @08:47:46.02 PSV.dial PTV @08:47:46.74 PTV.dial :PRV,avoiding => puck @08:47:47.46 PS.seek 0.000ml @08:48:13.70 PSV.dial :air @08:48:14.43 PS.seek 2.500ml @08:48:40.63 delay 3 seconds @08:48:43.64 PSV.dial PTV @08:48:44.35 PTV.dial :PRV,avoiding => puck @08:48:44.37 PS.seek 0.000ml @08:49:10.66 Pushing modified lysate into AIV Injection Loop @08:49:10.67 CBV.dial AIV @08:49:10.68 AIV.dial :bypass @08:49:10.69 PRV.dial CBV @08:49:10.70 PSV.dial :air @08:49:11.42 PS.seek 2.500ml @08:49:37.60 delay 3 seconds @08:49:40.61 PSV.dial :PML @08:49:41.05 PTV.dial :PRVmixing,avoiding => puck @08:49:41.82 PS.reconfigure PSslow1 @08:49:41.85 PS.seek 1.850ml @08:50:48.53 delay 5 seconds @08:50:53.54 AIV.dial :sample @08:50:54.06 AWV.select :waste @08:50:54.22 PS.reconfigure PSslow2 @08:50:54.25 PS.seek 1.310ml @08:52:44.29 delay 15 seconds @08:52:59.30 AIV.dial :bypass @08:52:59.84 PS.reconfigure PSconfig @08:52:59.87 PS.seek 0.000ml @08:53:14.01 PSV.dial :air @08:53:14.46 PS.seek 2.500ml @08:53:40.73 PSV.dial :PML @08:53:41.17 PS.seek 0.000ml @08:54:07.44 AIV.dial :coilToMFB @08:54:07.45 AWV.select :air @08:54:07.59 Extracting Nucleic Acid from AIV @08:54:07.61
Beginning samplePassD @08:54:07.62 SPE.hold 2:46:40,55.0C @08:54:07.64 ASV.dial :SPEdelivery @08:54:07.77
AWV.select :air @08:54:07.78 AIV.dial :bypass @08:54:07.79 PSV.dial PSR @08:54:08.18 delay 20 seconds @08:54:08.82
PSR.select :killMFB @08:54:08.92 PS.seek 1.500ml @08:54:25.07 delay 5 seconds @08:54:28.19 primeLysateINT @08:54:28.20 AWV.select :air @08:54:28.21 AIV.dial :coilToMFB ATV.dial :AIV @08:54:29.64 AS.reconfigure ASslow1 @08:54:29.67 AS.seek 0.239ml @08:54:30.08
PSV.dial :waste1 @08:54:30.53 PS.seek 0.000ml @08:54:46.63 PSV.dial PSR @08:54:47.09 PSR.select :air @08:54:47.18 PS.seek 2.500ml @08:55:13.41 PRV.dial CBV @08:55:13.43
CBV.dial PRV @08:55:13.44 PSV.dial :mixloop @08:55:14.46 CTV.dial :air,avoiding => intake @08:55:14.47 PTV.dial :PRVmixing @08:55:14.49 PS.seek 0.000ml @08:55:18.92 delay 10 seconds @08:55:28.93 ATV.dial :air2 @08:55:29.51 AS.seek 0.269ml @08:55:35.88 ATV.dial :waste2 @08:55:36.35 AS.reconfigure ASnorm @08:55:36.38 AS.seek empty @08:55:40.72
PSV.dial PSR @08:55:41.75 PSR.select :flush @08:55:41.85 PS.seek 1.500ml @08:55:50.54 Lysate primed! @08:55:50.55 AFV.select :holdingCoil @08:55:50.56 ATV.dial :air2 @08:55:50.95 AS.reconfigure ASslow1 @08:55:50.98 AS.seek 0.069ml @08:55:57.94
delay 5 seconds @08:56:02.95 PSV.dial :waste1 @08:56:03.40 PS.seek 0.000ml @08:56:05.62 delay 3 seconds @08:56:08.63 ATV.dial :AIV @08:56:09.21 AS.seek 0.469ml @08:56:19.47
PSV.dial PSR @08:56:19.92 PSR.select :air @08:56:20.02 PS.seek 2.500ml @08:56:46.31 PRV.dial CBV @08:56:46.33
CBV.dial PRV @08:56:46.34 PSV.dial :mixloop @08:56:47.36 CTV.dial :air,avoiding => intake @08:56:47.38 PTV.dial :PRVmixing @08:56:47.39 PS.seek 0.000ml @08:57:13.62 PSV.dial PSR @08:57:14.76 PSR.select :flush @08:57:14.86 PS.seek 1.500ml @08:57:30.87 delay 5 seconds @08:57:31.02 delay 5 seconds @08:57:35.88
PSV.dial :waste1 @08:57:36.07 Solid Phase Extraction Heater at 52.3C @08:57:36.08 ATV.dial ASV @08:57:36.65
PS.seek 0.000ml @08:57:37.04 ASV.dial :SPEdelivery @08:57:37.11 AS.seek 0.041ml @08:57:52.72
PSV.dial PSR @08:57:53.17 PSR.select :air @08:57:53.27 PS.seek 2.500ml @08:58:19.56 PRV.dial CBV @08:58:19.58
CBV.dial PRV @08:58:19.59 PSV.dial :mixloop @08:58:20.71 CTV.dial :air,avoiding => intake @08:58:20.72 PTV.dial :PRVmixing @08:58:20.74 PS.seek 0.000ml @08:58:47.07 PSV.dial PSR @08:58:48.07 PSR.select :flush @08:58:48.17 PS.seek 1.500ml @08:59:04.31 delay 5 seconds @08:59:04.64 delay 10 seconds @08:59:09.32
PSV.dial :waste1 @08:59:09.75 PS.seek 0.000ml @08:59:14.65 rinseLysateLineINT @08:59:14.66 AFV.select :carrierSolution @08:59:14.75 delay 2 seconds @08:59:16.76 AS.reconfigure ASconfig @08:59:16.79 AS.seek 1.000ml @08:59:25.87
PSV.dial PSR @08:59:26.31 PSR.select :air @08:59:26.41 PS.seek 2.500ml @08:59:52.68 PRV.dial CBV @08:59:52.70
CBV.dial PRV @08:59:52.71 PSV.dial :mixloop @08:59:53.71 CTV.dial :air,avoiding => intake @08:59:53.72 PTV.dial :PRVmixing @08:59:53.73 PS.reconfigure PSslow @08:59:53.77 PS.seek 1.200ml @09:00:13.00 delay 1 second @09:00:14.01 AFV.select :holdingCoil @09:00:14.10 delay 1 second @09:00:15.11 ATV.dial :AIV @09:00:15.44 AIV.dial :coilToMFB @09:00:15.45 AWV.select :waste @09:00:15.55 AS.reconfigure ASslow0 @09:00:15.59 AS.seek 0.000ml @09:00:20.74
PSV.dial :waste1 @09:00:22.03 PS.reconfigure PSconfig @09:00:22.07 PS.seek 0.000ml @09:00:35.08 PSV.dial :air @09:00:36.11 PS.seek 2.500ml @09:01:02.33 PSV.dial :mixloop @09:01:02.78 PS.reconfigure PSslow @09:01:02.81 PS.seek 0.000ml @09:01:54.18 PSV.dial :air @09:01:54.75 PS.reconfigure PSconfig @09:01:54.78 PS.seek 2.500ml @09:01:58.11 ATV.dial :air2 @09:01:58.67 AS.reconfigure ASslow1 @09:01:58.70 AS.seek 0.050ml @09:02:09.18 ATV.dial :AIV @09:02:09.84 AS.seek 0.010ml @09:02:18.23 ATV.dial :waste @09:02:19.52 AS.seek empty @09:02:21.08
PSV.dial :mixloop @09:02:21.51 PS.reconfigure PSslow @09:02:21.54 PS.seek 0.000ml @09:02:22.14 ethanol wash @09:02:22.15 ATV.dial :air2 @09:02:23.20 AS.reconfigure ASslow2 @09:02:23.23 AS.seek 0.009ml @09:02:33.76 delay 3 seconds @09:02:36.77 Pulling 0.4ml of Ethanol Wash @09:02:36.78 ATV.dial :ethanol @09:02:37.34 AS.reconfigure ASslow1 @09:02:37.37 AS.seek 0.409ml @09:03:12.89
Cleaning Collection Syringe @09:03:12.90 CSV.dial CSR @09:03:13.32 CSR.select :flush @09:03:13.42 CS.seek 4.000ml @09:03:24.40 delay 5 seconds @09:03:29.41 CSR.select :air @09:03:29.50 CS.seek 10.000ml @09:03:45.43 CSV.dial CTV @09:03:45.45
CTV.dial CSV @09:03:46.76 CBV.dial :waste1 @09:03:47.24 CS.seek 0.000ml @09:03:59.21 delay 3 seconds @09:04:02.22 ATV.dial :air1 @09:04:02.56 delay 3 seconds @09:04:05.62 AS.seek 0.434ml @09:04:10.98 delay 3 seconds @09:04:13.31
CSV.dial CSR @09:04:13.82 CSR.select :flush @09:04:13.92 CS.seek 4.000ml @09:04:13.99 ATV.dial :ethanol @09:04:14.39 AS.seek 0.419ml @09:04:17.70 delay 3 seconds @09:04:20.71 ATV.dial ASV @09:04:21.84 AS.seek 0.092ml @09:04:24.93
delay 5 seconds @09:04:29.94 CSR.select :air @09:04:30.03 CS.seek 10.000ml @09:04:46.08 CSV.dial CTV @09:04:46.10
CTV.dial CSV @09:04:46.62 CBV.dial :waste1 @09:04:46.64 CS.seek 0.000ml @09:05:12.82 Garaging FlushPuck in Collection Clamp @09:05:12.87 Elbow.reconfigure TightElbow @09:05:12.90 Elbow.seek CC @09:05:13.62 Elbow.reconfigure LooseElbow @09:05:13.70 Forearm.seek CC @09:05:14.67 CC.seek sealed @09:05:15.58 Hand.grip @09:05:16.33 CC.seek open @09:05:19.65 Forearm.seek retract @09:05:20.59 Elbow.reconfigure TightElbow @09:05:20.62 Elbow.seek garage @09:05:23.37 Elbow.reconfigure LooseElbow @09:05:23.45 Forearm.reconfigure TightForearm @09:05:23.48 Forearm.seek garage @09:05:24.64 Hand.release @09:05:25.37 Forearm.reconfigure LooseForearm @09:05:25.40 Forearm.seek retract @09:05:26.44 Whole Cell Archive sampling 1000ml @09:05:26.46 Loading wcr puck from tube 3 into Collection Clamp @09:05:26.61 SE.seek down @09:05:26.92 SC.reconfigure SCtight @09:05:26.96 SC.seek 3 @09:05:28.76 delay 3 seconds @09:05:29.28 SC.reconfigure SCloose @09:05:29.46 Elbow.reconfigure TightElbow @09:05:29.49 Elbow.seek 3 @09:05:31.34 Forearm.reconfigure TightForearm @09:05:31.37 Forearm.seek 3 + 800 counts @09:05:31.77 ATV.dial :waste2 @09:05:32.45 AS.reconfigure ASconfig @09:05:32.49 AS.seek empty @09:05:32.51 SE.reconfigure SEpick @09:05:32.60 SE.seek beamBreak @09:05:35.47 SE.configure SEconfig @09:05:35.60 Elbow.seek 3 @09:05:35.74 Forearm.seek 3 + 300 counts @09:05:36.05 Forearm.reconfigure LooseForearm @09:05:36.09 Hand.grip @09:05:36.85 SE.seek down @09:05:36.97 Forearm.seek retract @09:05:37.93 Elbow.seek CC @09:05:38.26 ATV.dial :air2 @09:05:38.63 AS.reconfigure ASslow1 @09:05:38.66 AS.seek 1.000ml @09:05:39.40 SE.coast @09:05:39.49 Elbow.reconfigure LooseElbow @09:05:39.58 Forearm.reconfigure TightForearm @09:05:39.61 Forearm.seek CC @09:05:40.74 CC.seek closed @09:05:44.51 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.93 @09:05:44.52 Hand.release @09:05:45.24 Forearm.reconfigure LooseForearm @09:05:45.27 Forearm.seek retract @09:05:46.19 SC.coast @09:05:46.22 collectSample 1000ml w/bubble point of 55psi @09:05:46.24 Priming 7ml Sleepy.power :sampler,:ON @09:05:46.25 Intake.select :close @09:05:46.26 Exhaust.select :close @09:05:46.27 SS.hold @09:05:46.41 Calibrating pressure sensors @09:05:46.42 CTV.dial :air @09:05:47.73 SSV.dial :spit,avoiding => exhaust @09:05:47.74 SS.reconfigure SSfast @09:05:47.77 SS.seek 5.00ml @09:05:50.75 SSV.dial CBV @09:05:50.76 CBV.dial SSV @09:05:52.14 delay 3.8 seconds @09:05:56.03 SSV.dial :spit @09:05:57.89 SS.seek 0.00ml @09:06:00.37 SSV.dial CBV @09:06:01.69 CTV.dial :intake @09:06:02.43 Waiting for external sampler to finish priming... @09:06:02.44 delayUntil "09:15:46.24" @09:09:03.13 delay 5 seconds @09:09:08.14 ATV.dial ASV @09:09:08.85 ASV.dial :SPEdelivery @09:09:08.87 AS.seek 0.020ml @09:12:29.00 delay 5 seconds @09:12:34.01 ATV.dial :air2 @09:12:34.77 AS.seek 0.520ml @09:14:17.02 delay 5 seconds @09:14:22.03 ATV.dial ASV @09:14:22.75 ASV.dial :wasteR @09:14:23.35 AS.seek 0.300ml @09:15:08.45 delay 20 seconds @09:15:28.46 AS.seek 0.070ml @09:15:46.25 SSV.dial CBV @09:15:46.27 CBV.dial SSV @09:15:46.28 Allowing 55s for 7.0ml of remaining 7.0ml @09:15:46.29 Intake.select :open @09:15:48.43 Intake @15.2psia, vacuum from 8.7 to 12.7psi @09:15:48.45 SS.reconfigure forSampling @09:15:48.48 SS.seek 7.00ml @09:15:53.88 Stroke completed -- waiting for pressure to equalize ... @09:15:54.52 Intake.select :close @09:15:54.66 SSV.dial :exhaust @09:15:55.44 Exhaust.select :open @09:15:55.54 SS.reconfigure SSexhale @09:15:55.58 SS.seek 0.00ml @09:15:59.60 Exhaust.select :close @09:15:59.71 Sampling 1000.0ml @09:15:59.72 Intake.select :close @09:15:59.73 Exhaust.select :close @09:15:59.74 SS.reconfigure SSconfig @09:15:59.77 SS.hold @09:15:59.93 CTV.dial :intake @09:15:59.95 SSV.dial CBV @09:15:59.97 CBV.dial SSV @09:16:00.73 Allowing 185s for 25.0ml of remaining 1000.0ml @09:16:00.74 Intake.select :open @09:16:02.87 Intake @15.0psia, vacuum from 8.5 to 12.5psi @09:16:02.89 SS.reconfigure forSampling @09:16:02.92 SS.seek 25.00ml @09:16:15.61 delay 20 seconds @09:16:16.52 Stroke completed -- waiting for pressure to equalize ... @09:16:17.16 Intake.select :close @09:16:17.30 SSV.dial :exhaust @09:16:18.08 Exhaust.select :open @09:16:18.18 SS.reconfigure SSexhale @09:16:18.22 SS.seek 0.00ml @09:16:26.80 Exhaust.select :close @09:16:26.90 SSV.dial CBV @09:16:26.92 CBV.dial SSV @09:16:27.67 Allowing 185s for 25.0ml of remaining 975.0ml @09:16:27.69 Intake.select :open @09:16:29.82 Intake @15.0psia, vacuum from 8.5 to 12.5psi @09:16:29.84 SS.reconfigure forSampling @09:16:29.87 SS.seek 25.00ml @09:16:35.62 ATV.dial :waste2 @09:16:36.26 AS.reconfigure ASconfig @09:16:36.29 AS.seek empty @09:16:40.71 Elution @09:16:40.72 ATV.dial :air1 @09:16:41.69 AS.reconfigure ASslow1 @09:16:41.72 AS.seek 0.049ml @09:16:43.67 Stroke completed -- waiting for pressure to equalize ... @09:16:44.31 Intake.select :close @09:16:44.45 SSV.dial :exhaust @09:16:45.22 Exhaust.select :open @09:16:45.32 SS.reconfigure SSexhale @09:16:45.36 SS.seek 0.00ml @09:16:52.31 delay 3 seconds @09:16:54.23 Exhaust.select :close @09:16:54.33 SSV.dial CBV @09:16:54.35 CBV.dial SSV @09:16:55.12 Allowing 185s for 25.0ml of remaining 950.0ml @09:16:55.13 Intake.select :open @09:16:55.32 ATV.dial :ElutionWater @09:16:55.90 AS.seek 0.109ml @09:16:57.26 Intake @15.1psia, vacuum from 8.6 to 12.6psi @09:16:57.28 SS.reconfigure forSampling @09:16:57.31 SS.seek 25.00ml @09:17:08.40 delay 3 seconds @09:17:11.31 Stroke completed -- waiting for pressure to equalize ... @09:17:11.41 ATV.dial ASV,via => air2 @09:17:11.95 Intake.select :close @09:17:12.10 SSV.dial :exhaust @09:17:12.86 Exhaust.select :open @09:17:12.96 SS.reconfigure SSexhale @09:17:13.00 SS.seek 0.00ml @09:17:13.07 ASV.dial :SPEelution @09:17:13.57 AFV.select :carrierSolution @09:17:13.72 AS.reconfigure ASnorm @09:17:13.75 AS.seek 0.759ml @09:17:22.01 Exhaust.select :close @09:17:22.11 SSV.dial CBV @09:17:22.13 CBV.dial SSV @09:17:22.89 Allowing 185s for 25.0ml of remaining 925.0ml @09:17:22.91 Intake.select :open @09:17:25.04 Intake @15.2psia, vacuum from 8.7 to 12.7psi @09:17:25.06 SS.reconfigure forSampling @09:17:25.09 SS.seek 25.00ml @09:17:39.07 Stroke completed -- waiting for pressure to equalize ... @09:17:39.71 Intake.select :close @09:17:40.35 SSV.dial :exhaust @09:17:41.12 Exhaust.select :open @09:17:41.22 SS.reconfigure SSexhale @09:17:41.26 SS.seek 0.00ml @09:17:47.24 delay 3 seconds @09:17:50.25 AFV.select :holdingCoil @09:17:50.34 Exhaust.select :close @09:17:50.42 AS.reconfigure ASslow1 @09:17:50.46 SSV.dial CBV @09:17:50.49 AS.seek 0.489ml @09:17:50.50 CBV.dial SSV @09:17:51.27 Allowing 185s for 25.0ml of remaining 900.0ml @09:17:51.28 Intake.select :open @09:17:53.41 Intake @15.2psia, vacuum from 8.7 to 12.7psi @09:17:53.43 SS.reconfigure forSampling @09:17:53.46 SS.seek 25.00ml @09:18:07.46 Stroke completed -- waiting for pressure to equalize ... @09:18:08.10 Intake.select :close @09:18:08.24 SSV.dial :exhaust @09:18:09.01 Exhaust.select :open @09:18:09.11 SS.reconfigure SSexhale @09:18:09.15 SS.seek 0.00ml @09:18:18.24 Exhaust.select :close @09:18:18.34 SSV.dial CBV @09:18:18.36 CBV.dial SSV @09:18:19.14 Allowing 185s for 25.0ml of remaining 875.0ml @09:18:19.16 Intake.select :open @09:18:21.29 Intake @15.2psia, vacuum from 8.7 to 12.7psi @09:18:21.31 SS.reconfigure forSampling @09:18:21.34 SS.seek 25.00ml @09:18:35.29 Stroke completed -- waiting for pressure to equalize ... @09:18:35.93 Intake.select :close @09:18:36.07 SSV.dial :exhaust @09:18:36.82 Exhaust.select :open @09:18:36.92 SS.reconfigure SSexhale @09:18:36.96 SS.seek 0.00ml @09:18:45.88 Exhaust.select :close @09:18:45.92 delay 5 seconds @09:18:45.98 SSV.dial CBV @09:18:46.00 CBV.dial SSV @09:18:46.77 Allowing 185s for 25.0ml of remaining 850.0ml @09:18:46.78 Intake.select :open @09:18:48.91 Intake @15.2psia, vacuum from 8.7 to 12.7psi @09:18:48.93 SS.reconfigure forSampling @09:18:48.96 SS.seek 25.00ml @09:18:50.98 AS.reconfigure ASslow2 @09:18:51.01 AS.seek 0.454ml @09:19:02.98 Stroke completed -- waiting for pressure to equalize ... @09:19:03.62 Intake.select :close @09:19:03.76 SSV.dial :exhaust @09:19:04.54 Exhaust.select :open @09:19:04.64 SS.reconfigure SSexhale @09:19:04.68 SS.seek 0.00ml @09:19:13.71 Exhaust.select :close @09:19:13.81 SSV.dial CBV @09:19:13.83 CBV.dial SSV @09:19:14.59 Allowing 185s for 25.0ml of remaining 825.0ml @09:19:14.61 Intake.select :open @09:19:16.74 Intake @15.2psia, vacuum from 8.7 to 12.7psi @09:19:16.76 SS.reconfigure forSampling @09:19:16.79 SS.seek 25.00ml @09:19:26.90 delay 1:00 @09:19:30.82 Stroke completed -- waiting for pressure to equalize ... @09:19:31.46 Intake.select :close @09:19:31.60 SSV.dial :exhaust @09:19:32.37 Exhaust.select :open @09:19:32.47 SS.reconfigure SSexhale @09:19:32.51 SS.seek 0.00ml @09:19:41.51 Exhaust.select :close @09:19:41.61 SSV.dial CBV @09:19:41.63 CBV.dial SSV @09:19:42.40 Allowing 185s for 25.0ml of remaining 800.0ml @09:19:42.42 Intake.select :open @09:19:44.55 Intake @15.3psia, vacuum from 8.8 to 12.8psi @09:19:44.57 SS.reconfigure forSampling @09:19:44.60 SS.seek 25.00ml @09:19:58.60 Stroke completed -- waiting for pressure to equalize ... @09:19:59.24 Intake.select :close @09:19:59.38 SSV.dial :exhaust @09:20:00.13 Exhaust.select :open @09:20:00.23 SS.reconfigure SSexhale @09:20:00.27 SS.seek 0.00ml @09:20:09.25 Exhaust.select :close @09:20:09.35 SSV.dial CBV @09:20:09.37 CBV.dial SSV @09:20:10.13 Allowing 185s for 25.0ml of remaining 775.0ml @09:20:10.14 Intake.select :open @09:20:12.28 Intake @15.3psia, vacuum from 8.8 to 12.8psi @09:20:12.30 SS.reconfigure forSampling @09:20:12.33 SS.seek 25.00ml @09:20:26.61 Stroke completed -- waiting for pressure to equalize ... @09:20:26.91 pushing eluate to holding coil @09:20:26.97 AS.seek 0.294ml @09:20:27.25 Intake.select :close @09:20:27.40 SSV.dial :exhaust @09:20:28.16 Exhaust.select :open @09:20:28.26 SS.reconfigure SSexhale @09:20:28.30 SS.seek 0.00ml @09:20:37.13 Exhaust.select :close @09:20:37.23 SSV.dial CBV @09:20:37.25 CBV.dial SSV @09:20:38.53 Allowing 185s for 25.0ml of remaining 750.0ml @09:20:38.54 Intake.select :open @09:20:40.67 Intake @15.3psia, vacuum from 8.8 to 12.8psi @09:20:40.69 SS.reconfigure forSampling @09:20:40.72 SS.seek 25.00ml @09:20:45.32 Vacuum too high -- waiting up to 40s @09:21:25.45 SS.reconfigure SSconfig @09:21:25.49 SS.hold @09:21:26.32 Intake.select :close @09:21:26.47 ShallowSampler::Clogged in WCR -- Intake @15.4psia, & Outlet @2.1psia -- no progress after 256.1ml @09:21:26.48 SSV.dial :exhaust @09:21:27.25 Exhaust.select :open @09:21:27.35 SS.reconfigure SSexhale @09:21:27.39 SS.seek 0.00ml @09:21:31.06 Exhaust.select :close @09:21:31.17 Sampled 256.1ml @09:21:31.18 collectSample: Complete @09:21:31.19 Drying puck before fixing for 04:00.00 @09:21:31.20 CBV.dial :waste2b @09:21:31.92 delay 1:00 @09:21:34.16 Sleepy.power :sampler,:OFF @09:22:31.92 CSV.dial :air @09:22:32.37 CS.reconfigure CSfast @09:22:32.40 CS.seek 10.000ml @09:22:56.51 CBV.dial between CSV and waste2b @09:22:56.84 Pumping wcr puck from tube 3 up to 55psi... @09:22:56.89 CBV.dial SSV @09:22:58.32 CBV.dial between CSV and waste2b @09:22:59.25 CSV.dial between air and CTV @09:22:59.63 CS.seek 6.857ml @09:23:07.62 CTV.dial CSV,avoiding => air @09:23:07.64 CSV.dial CTV @09:23:08.38 CS.reconfigure CSpressurize @09:23:08.41 CS.seek 0.000ml @09:23:10.45 delay 30 seconds @09:23:26.33 CSV.dial :air @09:23:27.07 CS.reconfigure CSfast @09:23:27.10 CS.seek 10.000ml @09:23:40.46 ATV.dial :waste,via => air3 @09:23:41.91 AS.reconfigure ASconfig @09:23:41.94 AS.seek empty @09:23:51.25 CSV.dial between air and CTV @09:23:51.54 CS.seek 2.557ml @09:23:59.53 Parking Bleach in LysateSource line and SPEcolumn @09:23:59.54 ATV.dial :air3 @09:24:00.11 AS.reconfigure ASslow1 @09:24:00.14 AS.seek 0.029ml @09:24:06.56 ATV.dial :ElutionWater @09:24:07.05 AS.seek 0.059ml @09:24:09.30 CTV.dial CSV,avoiding => air @09:24:09.31 CSV.dial CTV @09:24:09.60 CS.reconfigure CSpressurize @09:24:09.63 CS.seek 0.000ml @09:24:13.19 CBV.dial :waste2b @09:24:13.43 ATV.dial :air3 @09:24:13.51 CS.reconfigure CSconfig @09:24:13.54 CS.seek empty @09:24:13.81 AS.seek 0.069ml @09:24:16.11 delay 2 seconds @09:24:17.70 delay 1:00 @09:24:18.12 ATV.dial :bleach @09:24:18.50 AS.seek 0.709ml @09:25:17.71 CTV.dial :air,avoiding => intake @09:25:18.99 Fix #1 of 2 (with rnal) for 02:00.00 @09:25:19.00 CS Pulling 2ml of rnal CSV.dial CSR @09:25:19.43 CSR.select :rnal @09:25:19.53 CS.seek 2.000ml @09:25:25.45 delay 5 seconds @09:25:30.46 CSR.select :air @09:25:30.55 CS.reconfigure CSfast @09:25:30.59 CS.seek 10.000ml @09:25:49.83 CBV.dial between CSV and waste2b @09:25:50.17 Pumping wcr puck from tube 3 up to 55psi... @09:25:50.22 CBV.dial SSV @09:25:51.63 CBV.dial between CSV and waste2b @09:25:52.57 CSV.dial between air and CTV @09:25:53.23 CS.seek 6.761ml @09:26:01.43 CTV.dial CSV,avoiding => air @09:26:01.45 CSV.dial CTV @09:26:02.75 CS.reconfigure CSpressurize @09:26:02.78 CS.seek 0.000ml @09:26:19.94 delay 2:00 @09:26:29.30 delay 3 seconds @09:26:32.31 ATV.dial :air3 @09:26:32.66 delay 3 seconds @09:26:35.72 AS.seek 0.734ml @09:26:41.08 delay 3 seconds @09:26:44.09 ATV.dial :bleach @09:26:44.58 AS.seek 0.719ml @09:26:47.86 delay 3 seconds @09:26:50.87 ATV.dial :AIV @09:26:52.06 AS.reconfigure ASconfig @09:26:52.09 AS.seek 0.015ml @09:27:33.41 ATV.dial :waste @09:27:34.70 AS.seek empty @09:27:35.91 ATV.dial :air3 @09:27:36.99 AS.reconfigure ASslow1 @09:27:37.03 AS.seek 0.009ml @09:27:39.45 delay 2 seconds @09:27:41.46 ATV.dial :bleach @09:27:41.83 AS.seek 0.579ml @09:28:19.95 CBV.dial :waste2b @09:28:20.27 CS.reconfigure CSconfig @09:28:20.30 CS.seek empty @09:28:21.35 delay 1:00 @09:29:21.36 CTV.dial :air,avoiding => intake @09:29:22.63 Fix #2 of 2 (with rnal) for 02:00.00 @09:29:22.64 CS Pulling 2ml of rnal CSV.dial CSR @09:29:23.07 CSR.select :rnal @09:29:23.17 CS.seek 2.000ml @09:29:29.07 delay 5 seconds @09:29:34.08 CSR.select :air @09:29:34.17 CS.reconfigure CSfast @09:29:34.21 CS.seek 10.000ml @09:29:38.34 delay 3 seconds @09:29:41.35 ATV.dial :air3 @09:29:41.70 delay 3 seconds @09:29:44.76 AS.seek 0.604ml @09:29:50.10 delay 3 seconds @09:29:53.11 ATV.dial :bleach @09:29:53.29 CBV.dial between CSV and waste2b @09:29:53.50 AS.seek 0.589ml @09:29:53.63 Pumping wcr puck from tube 3 up to 55psi... @09:29:53.68 CBV.dial SSV @09:29:55.09 CBV.dial between CSV and waste2b @09:29:56.03 CSV.dial between air and CTV @09:29:56.69 CS.seek 6.599ml @09:29:56.81 delay 3 seconds @09:29:59.82 ATV.dial :ASV @09:30:00.81 ASV.dial :wasteR @09:30:01.47 AS.seek 0.000ml @09:30:05.27 CTV.dial CSV,avoiding => air @09:30:05.29 CSV.dial CTV @09:30:06.66 CS.reconfigure CSpressurize @09:30:06.69 CS.seek 0.000ml @09:30:23.18 delay 2:00 @09:32:01.91 delay 1:00 @09:32:23.19 CBV.dial :waste2b @09:32:23.52 CS.reconfigure CSconfig @09:32:23.58 CS.seek empty @09:32:24.94 delay 1:00 @09:33:01.92 AFV.select :carrierSolution @09:33:02.01 delay 2 seconds @09:33:04.02 AS.reconfigure ASconfig @09:33:04.05 AS.seek 1.000ml @09:33:24.95 CTV.dial :air,avoiding => intake @09:33:26.25 Storing wcr puck in Collection Clamp to tube 2 @09:33:26.36 SE.seek down @09:33:26.68 SC.reconfigure SCtight @09:33:26.71 SC.seek 2 @09:33:28.94 SC.reconfigure SCloose @09:33:29.12 Elbow.reconfigure TightElbow @09:33:29.15 Elbow.seek 2 @09:33:30.77 Forearm.reconfigure TightForearm @09:33:30.80 Forearm.seek 2 + 800 counts @09:33:31.80 SE.reconfigure SEpick @09:33:31.85 SE.seek beamBreak @09:33:33.11 SE.configure SEconfig @09:33:33.23 Elbow.reconfigure LimpElbow @09:33:33.27 Forearm.reconfigure LooseForearm @09:33:33.31 Forearm.seek 2 @09:33:33.53 Elbow.hold @09:33:33.59 Elbow.reconfigure TightElbow @09:33:33.67 SE.seek 18.03 Pucks below Top Plate @09:33:34.03 Forearm.coast @09:33:34.07 Forearm.seek 2 + 342 counts @09:33:34.36 Forearm.reconfigure TightForearm @09:33:34.40 Forearm.seek 2 + 800 counts @09:33:34.57 SE.seek 19.03 Pucks below Top Plate @09:33:35.12 Forearm.reconfigure LooseForearm @09:33:35.16 Forearm.seek retract @09:33:36.08 Elbow.seek CC @09:33:37.67 Elbow.reconfigure LooseElbow @09:33:38.29 Forearm.seek CC @09:33:39.21 CC.seek sealed @09:33:40.15 Hand.grip @09:33:40.90 CC.seek open @09:33:44.26 Forearm.seek retract @09:33:45.22 Elbow.reconfigure TightElbow @09:33:45.25 Elbow.seek 2 @09:33:46.85 Elbow.reconfigure LimpElbow @09:33:46.89 Forearm.seek 2 @09:33:47.90 Elbow.hold @09:33:47.95 Elbow.reconfigure TightElbow @09:33:47.99 Hand.release @09:33:48.85 Forearm.coast @09:33:48.89 Forearm.seek 2 + 316 counts @09:33:49.13 Forearm.reconfigure TightForearm @09:33:49.17 Forearm.seek 2 + 800 counts @09:33:49.49 SE.seek down @09:33:50.28 SE.coast @09:33:50.59 Forearm.reconfigure LooseForearm @09:33:50.62 Forearm.seek retract @09:33:51.54 SC.coast @09:33:51.58 Starting finalPurge Loading FlushPuck into Collection Clamp from FlushPuck::Garage @09:33:51.74 Elbow.seek FlushPuck::Garage @09:33:53.56 Elbow.reconfigure LooseElbow @09:33:53.64 Forearm.seek garage @09:33:54.65 Hand.grip @09:33:55.49 Forearm.seek retract @09:33:56.52 Elbow.reconfigure TightElbow @09:33:56.55 Elbow.seek CC @09:33:59.33 Elbow.reconfigure LooseElbow @09:33:59.41 Forearm.reconfigure TightForearm @09:33:59.44 Forearm.seek CC @09:34:00.58 CC.seek closed @09:34:02.75 delay 1 second @09:34:03.76 AFV.select :holdingCoil @09:34:03.87 delay 1 second @09:34:04.34
Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 2.02 @09:34:04.40 Hand.release @09:34:04.88 ATV.dial :ASV @09:34:04.89 ASV.dial :wasteR @09:34:04.90 AS.reconfigure ASslow1 @09:34:04.93 AS.seek 0.000ml @09:34:05.19
Forearm.reconfigure LooseForearm @09:34:05.22 Forearm.seek retract @09:34:06.20 Flushing the Collection Valve::Manifold @09:34:06.30 CS Pulling 5ml of flush CSV.dial CSR @09:34:06.81 CSR.select :flush @09:34:06.91 CS.seek 5.000ml @09:34:20.40 delay 5 seconds @09:34:25.41 CSR.select :air @09:34:25.55 CS.reconfigure CSfast @09:34:25.58 CS.seek 7.500ml @09:34:32.12 CSV.dial :waste @09:34:33.39 CS.reconfigure CSconfig @09:34:33.42 CS.seek 0.000ml @09:34:53.34 Flushing lower manifold's waste1 port @09:34:53.43 CSV.dial :flush @09:34:54.46 CS.reconfigure CSfast @09:34:54.49 CS.seek 5.000ml @09:35:06.77 delay 5 seconds @09:35:11.78 CSV.dial :air @09:35:12.26 CS.seek 6.999ml @09:35:17.69 CSV.dial CTV @09:35:17.70
CTV.dial CSV @09:35:19.02 CBV.dial :waste1 @09:35:19.77 CS.seek 0.000ml @09:35:36.37 Flushing lower manifold's waste2b port @09:35:36.47 CSV.dial :flush @09:35:37.27 CS.seek 5.000ml @09:35:49.55 delay 5 seconds @09:35:54.56 CSV.dial :air @09:35:55.10 CS.seek 7.000ml @09:36:00.46 CSV.dial CTV @09:36:00.48
CTV.dial CSV @09:36:00.91 CBV.dial :waste2b @09:36:01.66 CS.seek 0.000ml @09:36:18.21 Flushing upper manifold @09:36:18.30 CSV.dial :flush @09:36:19.09 CS.seek 5.000ml @09:36:31.33 delay 5 seconds @09:36:36.34 CSV.dial :air @09:36:36.88 CS.seek 6.999ml @09:36:42.30 CSV.dial CBV @09:36:42.32
CBV.dial CSV @09:36:43.44 CTV.dial :air @09:36:44.70 CS.seek 0.000ml @09:37:01.36 delay 7 seconds @09:37:08.37 CS.seek 3.000ml @09:37:16.14 CSV.dial :waste @09:37:16.65 CS.seek 0.000ml @09:37:24.33 CSV.dial :flush @09:37:24.76 CS.seek 3.000ml @09:37:29.18 delay 20 seconds @09:37:32.42
delay 5 seconds @09:37:37.43 CSV.dial :air @09:37:37.91 CS.seek 5.000ml @09:37:43.36 CSV.dial :air @09:37:43.38 CS.seek 0.000ml @09:37:49.19 ATV.dial :air3 @09:37:50.30 AS.seek 0.590ml @09:37:55.61
delay 7 seconds @09:38:02.62 CS.seek 3.000ml @09:38:10.39 CSV.dial :waste @09:38:11.20 CS.seek 0.000ml @09:38:18.89 Disinfecting external Intake with mfbkill @09:38:18.98 CSV.dial CSR @09:38:22.06 CSR.select :mfbkill @09:38:22.16 CS.seek 8.000ml @09:38:41.26 delay 5 seconds @09:38:46.27 CSR.select :air @09:38:46.36 CS.seek 10.000ml @09:38:51.89 CSV.dial CBV @09:38:51.91
CBV.dial CSV @09:38:52.90 CTV.dial :intake @09:38:53.62 Intake.select :open @09:38:53.72 CS.seek 2.000ml @09:39:12.83 Intake.select :close @09:39:12.92 CTV.dial :air @09:39:13.68 CS.reconfigure CSconfig @09:39:13.71 CS.seek 0.000ml @09:39:19.54 Flushing lower manifold's waste1 port @09:39:19.55 CS Pulling 4ml of mfbkill @09:39:19.56 CSV.dial CSR @09:39:20.62 CSR.select :mfbkill @09:39:20.72 CS.seek 4.000ml @09:39:31.73 delay 5 seconds @09:39:36.74 CSR.select :air @09:39:36.83 CS.reconfigure CSfast @09:39:36.87 CS.seek 10.000ml @09:39:51.01 delay 3 seconds @09:39:51.49 CSV.dial CTV @09:39:51.51
CTV.dial CSV @09:39:52.82 CBV.dial :waste1 @09:39:53.27 CS.seek 0.000ml @09:39:54.02 ATV.dial :ASV @09:39:55.10 ASV.dial :wasteR @09:39:55.16 AS.seek 0.090ml @09:40:16.57
Flushing lower manifold's waste2b port @09:40:16.58 CS Pulling 4ml of mfbkill @09:40:16.59 CSV.dial CSR @09:40:17.09 CSR.select :mfbkill @09:40:17.19 CS.reconfigure CSconfig @09:40:17.23 CS.seek 4.000ml @09:40:28.29 delay 5 seconds @09:40:33.30 CSR.select :air @09:40:33.39 CS.reconfigure CSfast @09:40:33.43 CS.seek 10.000ml @09:40:48.41 CTV.dial CSV @09:40:48.43 CSV.dial CTV @09:40:48.94
CBV.dial :waste2b @09:40:49.69 CS.seek 0.000ml @09:41:13.16 Killing upper manifold @09:41:13.17 CS Pulling 4ml of mfbkill @09:41:13.18 CSV.dial CSR @09:41:13.68 CSR.select :mfbkill @09:41:13.78 CS.reconfigure CSconfig @09:41:13.82 CS.seek 4.000ml @09:41:24.73 delay 5 seconds @09:41:29.74 CSR.select :air @09:41:29.83 CS.reconfigure CSfast @09:41:29.87 CS.seek 10.000ml @09:41:37.40 delay 30 seconds @09:41:44.29 CSV.dial CBV @09:41:44.31
CBV.dial CSV @09:41:45.56 CTV.dial :air @09:41:46.84 CS.seek 0.000ml @09:42:07.41 ATV.dial :extSrc @09:42:08.03 delay 3 seconds @09:42:10.29
delay 7 seconds @09:42:11.04 ATV.dial :ElutionWater @09:42:11.98 AS.seek 0.120ml @09:42:17.30
CS.seek 3.000ml @09:42:18.41 ATV.dial :air3 @09:42:18.79 AS.seek 0.130ml @09:42:21.14 delay 2 seconds @09:42:23.15 ATV.dial :bleach @09:42:23.52 AS.seek 0.230ml @09:42:24.94
CSV.dial :waste @09:42:25.67 CS.seek 0.000ml @09:42:33.44 Flushing upper manifold @09:42:33.53 CSV.dial :flush @09:42:34.06 CS.seek 5.000ml @09:42:44.16 delay 3 seconds @09:42:46.22
delay 5 seconds @09:42:47.18 ATV.dial :air3 @09:42:47.54 delay 3 seconds @09:42:50.59 AS.seek 0.255ml @09:42:51.24
CSV.dial :air @09:42:51.73 CS.seek 7.000ml @09:42:55.93 delay 3 seconds @09:42:57.12 CSV.dial CBV @09:42:57.13
CBV.dial CSV @09:42:58.21 CTV.dial :air @09:42:58.22 CS.seek 0.000ml @09:42:58.94 ATV.dial :bleach @09:42:59.33 AS.seek 0.240ml @09:43:02.65 delay 3 seconds @09:43:05.66 ATV.dial :ASV @09:43:06.64 ASV.dial :SPEdelivery @09:43:07.11 AFV.select :carrierSolution @09:43:07.21 delay 2 seconds @09:43:09.22 AS.reconfigure ASconfig @09:43:09.25 AS.seek 0.500ml @09:43:14.79
delay 7 seconds @09:43:21.80 CS.seek 3.000ml @09:43:24.68 delay 1 second @09:43:25.69 AFV.select :holdingCoil @09:43:25.83 AS.reconfigure ASslow1 @09:43:25.86 AS.seek 0.170ml @09:43:29.45
CSV.dial :waste @09:43:29.96 CS.seek 0.000ml @09:43:37.73 CSV.dial :flush @09:43:38.16 CS.seek 3.000ml @09:43:45.79 delay 5 seconds @09:43:50.80 CSV.dial :air @09:43:51.28 CS.seek 4.999ml @09:43:56.67 CSV.dial :air @09:43:56.69 CS.seek 0.000ml @09:44:08.76 delay 7 seconds @09:44:15.77 CS.seek 3.000ml @09:44:23.42 CSV.dial :waste @09:44:24.22 CS.seek 0.000ml @09:44:32.01 Flushing lower manifold's waste1 port @09:44:32.10 CSV.dial :flush @09:44:32.61 CS.seek 5.000ml @09:44:33.42 SPE.hold 2:46:40,95.0C @09:44:33.44 delay 3:00 @09:44:45.16
delay 5 seconds @09:44:50.17 CSV.dial :air @09:44:50.65 CS.seek 6.999ml @09:44:56.23 CSV.dial CTV @09:44:56.25
CTV.dial CSV @09:44:57.62 CBV.dial :waste1 @09:44:58.09 CS.seek 0.000ml @09:45:15.23 Flushing lower manifold's waste2b port @09:45:15.32 CSV.dial :flush @09:45:16.14 CS.seek 5.000ml @09:45:28.69 delay 5 seconds @09:45:33.70 CSV.dial :air @09:45:34.26 CS.seek 7.000ml @09:45:39.79 CSV.dial CTV @09:45:39.81
CTV.dial CSV @09:45:40.26 CBV.dial :waste2b @09:45:41.05 CS.seek 0.000ml @09:45:58.22 Disinfecting external Intake with kill @09:45:58.31 CSV.dial CSR @09:45:58.75 CSR.select :kill @09:45:58.85 CS.seek 8.000ml @09:46:18.57 delay 5 seconds @09:46:23.58 CSR.select :air @09:46:23.67 CS.seek 10.000ml @09:46:29.22 CSV.dial CBV @09:46:29.24
CBV.dial CSV @09:46:30.29 CTV.dial :intake @09:46:31.02 Intake.select :open @09:46:31.12 CS.seek 2.000ml @09:46:50.91 Intake.select :close @09:46:51.00 CTV.dial :air @09:46:51.75 CS.reconfigure CSconfig @09:46:51.78 CS.seek 0.000ml @09:46:57.69 CSV.dial CSR @09:46:58.99 CSR.select :flush @09:46:59.09 CS.seek 5.000ml @09:47:12.56 delay 5 seconds @09:47:17.57 CSR.select :air @09:47:17.67 CS.seek 10.000ml @09:47:31.09 CSV.dial CBV @09:47:32.31 CS.seek 0.000ml @09:47:33.45 AFV.select :carrierSolution @09:47:33.54 delay 2 seconds @09:47:35.55 AS.reconfigure ASconfig @09:47:35.58 AS.seek 1.000ml @09:47:58.44
Garaging FlushPuck in Collection Clamp @09:47:58.50 Elbow.reconfigure TightElbow @09:47:58.53 Elbow.seek CC @09:47:58.63 Elbow.reconfigure LooseElbow @09:47:58.71 Forearm.seek CC @09:47:59.71 CC.seek sealed @09:48:00.66 Hand.grip @09:48:01.37 CC.seek open @09:48:04.68 Forearm.seek retract @09:48:05.65 Elbow.reconfigure TightElbow @09:48:05.68 Elbow.seek garage @09:48:08.53 Elbow.reconfigure LooseElbow @09:48:08.61 Forearm.reconfigure TightForearm @09:48:08.64 Forearm.seek garage @09:48:09.80 Hand.release @09:48:10.53 Forearm.reconfigure LooseForearm @09:48:10.56 Forearm.seek retract @09:48:24.25 delay 1 second @09:48:25.26 AFV.select :holdingCoil @09:48:25.35 delay 1 second @09:48:26.36 ASV.dial :wasteD @09:48:27.37 AS.seek 0.000ml @09:49:25.94 Priming Eluate @09:49:25.95 AFV.select :carrierSolution @09:49:26.04 delay 2 seconds @09:49:28.05 AS.seek 0.500ml @09:49:57.55 delay 1 second @09:49:58.56 AFV.select :holdingCoil @09:49:58.65 ATV.dial ASV @09:49:58.66 ASV.dial :SPErecovery @09:49:59.31 Swish #1 of 2 @09:49:59.37 AS.reconfigure ASslow0 @09:49:59.40 AS.seek 0.600ml @09:50:09.95 AS.seek 0.500ml @09:50:20.44 Swish #2 of 2 @09:50:20.50 AS.seek 0.600ml @09:50:31.03 AS.seek 0.500ml @09:50:41.50 delay 3 seconds @09:50:44.56 AS.reconfigure ASslow1 @09:50:44.59 AS.seek 0.676ml @09:51:20.79 delay 3 seconds @09:51:23.80 Eluate primed @09:51:23.81 ATV.dial :waste2 @09:51:24.35 AS.reconfigure ASnorm @09:51:24.38 AS.seek 0.000ml @09:51:59.26 SPE.coast @09:51:59.33 ASV.dial :SPErecovery @09:51:59.35 Setting up PCR for HF183em -- writing /var/log/esp/HF183em14mar1809h1ml.pcr @09:51:59.36 Building PCR Slug from ASV with MM2 mix, PP4 primer @09:51:59.37 Priming with carrier @09:51:59.38 ATV.dial ARV @09:52:00.09 ARV.dial :carrier @09:52:00.37 AS.seek 0.500ml @09:52:26.32 AFV.select :carrierSolution @09:52:26.41 delay 2 seconds @09:52:28.42 AS.reconfigure ASconfig @09:52:28.46 AS.seek 1.000ml @09:52:57.91 delay 1 second @09:52:58.92 AFV.select :holdingCoil @09:52:59.01 delay 1 second @09:53:00.02 ATV.dial :waste @09:53:01.38 AS.reconfigure ASnorm @09:53:01.41 AS.seek empty @09:53:52.85 AFV.select :carrierSolution @09:53:52.95 delay 2 seconds @09:53:54.96 AS.reconfigure ASconfig @09:53:54.99 AS.seek 0.500ml @09:54:24.54 delay 1 second @09:54:25.55 AFV.select :holdingCoil @09:54:26.16 Building Slug @09:54:26.17 ATV.dial :ARV @09:54:27.43 delay 2 seconds @09:54:29.44 ARV.dial :air1 @09:54:29.77 delay 2 seconds @09:54:31.83 AS.reconfigure ASslow2 @09:54:31.86 AS.seek 0.506ml @09:54:38.18 delay 2 seconds @09:54:40.19 ARV.dial :MM2 @09:54:41.43 delay 2 seconds @09:54:43.44 Adding MM2 @09:54:43.50 AS.seek 0.524ml @09:55:02.08 delay 25 seconds @09:55:27.09 ARV.dial :PP4 @09:55:27.50 Adding PP4 @09:55:27.51 delay 2 seconds @09:55:29.57 AS.seek 0.530ml @09:55:35.94 delay 10 seconds @09:55:45.95 ARV.dial :air3 @09:55:46.39 adding ARV air @09:55:46.40 delay 2 seconds @09:55:48.46 AS.seek 0.536ml @09:55:54.92 delay 5 seconds @09:55:59.93 ARV.dial :carrier @09:56:00.97 delay 7 seconds @09:56:08.03 AS.seek 0.594ml @09:57:07.45 delay 5 seconds @09:57:12.46 ATV.dial :ASV @09:57:12.88 delay 2 seconds @09:57:14.94 AS.seek 0.600ml @09:57:21.27 delay 4 seconds @09:57:25.28 ATV.dial ARV @09:57:25.61 delay 2 seconds @09:57:27.62 ARV.dial :carrier @09:57:27.63 delay 2 seconds @09:57:29.68 AS.seek 0.650ml @09:58:21.01 delay 4 seconds @09:58:25.02 ATV.dial PCR @09:58:25.51 ASV.dial :waste1 @09:58:26.19 ARV.dial :air1 @09:58:26.63 Positioning Slug by 0.324ml @09:58:26.68 AS.seek 0.326ml @10:03:57.31 delay 5 seconds @10:04:02.32 ATV.dial ARV @10:04:02.84 ASV.dial :PCRlock @10:04:03.17 delay 5 seconds @10:04:08.18 Running HF183em ProfileEM60C2 thermocycling @10:04:08.19 PCR.program ProfileEM60C2 @10:04:08.30 ProfileEM60C2 should complete in < 2:11:38 @10:04:08.31 PCR.run 2:49:32.5 @11:59:59.82 PCR.off @12:00:00.88 PCR for HF183em completed -- closed /var/log/esp/HF183em14mar1809h1ml.pcr @12:00:00.93 Cleaning PCR AFV.select :carrierSolution @12:00:01.02 delay 2 seconds @12:00:03.03 AS.reconfigure ASconfig @12:00:03.07 AS.seek 0.500ml @12:00:13.52 delay 1 second @12:00:14.53 AFV.select :holdingCoil @12:00:14.62 ASV.dial :waste1 @12:00:15.37 ATV.dial :air3 @12:00:16.31 AS.reconfigure ASslow1 @12:00:16.34 AS.seek 0.510ml @12:00:18.63 delay 2 seconds @12:00:20.64 ATV.dial :bleach @12:00:21.12 AS.seek 0.810ml @12:01:22.55 delay 3 seconds @12:01:25.56 ATV.dial :air3 @12:01:25.88 delay 3 seconds @12:01:28.94 AS.seek 0.835ml @12:01:34.29 delay 3 seconds @12:01:37.30 ATV.dial :bleach @12:01:37.70 AS.seek 0.820ml @12:01:41.02 delay 3 seconds @12:01:44.03 ARV.dial :waste @12:01:44.51 delay 3 seconds @12:01:47.52 ATV.dial :ARV @12:01:48.25 AS.reconfigure ASconfig @12:01:48.29 AS.seek 0.470ml @12:02:08.97 AFV.select :carrierSolution @12:02:09.07 delay 2 seconds @12:02:11.08 AS.seek 1.000ml @12:02:42.28 delay 1 second @12:02:43.29 AFV.select :holdingCoil @12:02:43.38 delay 1 second @12:02:44.39 AS.reconfigure ASfast @12:02:44.42 AS.seek 0.000ml @12:03:12.16 AFV.select :carrierSolution @12:03:12.27 delay 2 seconds @12:03:14.28 AS.reconfigure ASconfig @12:03:14.31 AS.seek 1.000ml @12:04:12.94 delay 1 second @12:04:13.95 AFV.select :holdingCoil @12:04:14.04 delay 1 second @12:04:15.05 AS.reconfigure ASfast @12:04:15.08 AS.seek 0.000ml @12:04:42.80 AFV.select :carrierSolution @12:04:42.90 delay 2 seconds @12:04:44.91 AS.reconfigure ASconfig @12:04:44.94 AS.seek 1.000ml @12:05:43.56 delay 1 second @12:05:44.57 AFV.select :holdingCoil @12:05:44.66 delay 1 second @12:05:45.67 AS.reconfigure ASfast @12:05:45.70 AS.seek 0.000ml @12:06:13.39 ARV.dial between carrier and air1 @12:06:13.79 ATV.dial :air3 @12:06:14.73 AS.reconfigure ASslow1 @12:06:14.76 AS.seek 0.010ml @12:06:17.19 delay 2 seconds @12:06:19.19 ATV.dial :bleach @12:06:19.58 AS.seek 0.810ml @12:09:02.96 delay 3 seconds @12:09:05.97 ATV.dial :air3 @12:09:06.32 delay 3 seconds @12:09:09.38 AS.seek 0.835ml @12:09:14.69 delay 3 seconds @12:09:17.70 ATV.dial :bleach @12:09:18.09 AS.seek 0.820ml @12:09:21.38 delay 3 seconds @12:09:24.39 ATV.dial PCR @12:09:24.70 AS.reconfigure ASconfig @12:09:24.73 AS.seek empty @12:10:12.96 PCR.program :bleachProfile @12:10:13.01 PCR program should complete in < 2:45 @12:10:13.02 PCR.run 10:00 @12:13:11.21 PCR.off @12:13:11.24 AFV.select :carrierSolution @12:13:11.33 delay 2 seconds @12:13:13.34 AS.seek 1.000ml @12:14:11.98 delay 1 second @12:14:12.99 AFV.select :holdingCoil @12:14:13.08 delay 1 second @12:14:14.09 AS.reconfigure ASfast @12:14:14.12 AS.seek 0.000ml @12:14:41.84 delay 3 seconds @12:14:44.85 AFV.select :carrierSolution @12:14:44.94 delay 2 seconds @12:14:46.95 AS.reconfigure ASconfig @12:14:46.98 AS.seek 1.000ml @12:15:45.60 delay 1 second @12:15:46.61 AFV.select :holdingCoil @12:15:46.70 delay 1 second @12:15:47.71 AS.reconfigure ASfast @12:15:47.74 AS.seek 0.000ml @12:16:15.53 delay 3 seconds @12:16:18.54 AFV.select :carrierSolution @12:16:18.63 delay 2 seconds @12:16:20.64 AS.reconfigure ASconfig @12:16:20.67 AS.seek 1.000ml @12:17:19.27 delay 1 second @12:17:20.28 AFV.select :holdingCoil @12:17:20.37 delay 1 second @12:17:21.38 AS.reconfigure ASfast @12:17:21.41 AS.seek 0.000ml @12:17:49.12 delay 3 seconds @12:17:52.13 AFV.select :carrierSolution @12:17:52.22 delay 2 seconds @12:17:54.23 AS.reconfigure ASconfig @12:17:54.26 AS.seek 1.000ml @12:18:52.87 delay 1 second @12:18:53.88 AFV.select :holdingCoil @12:18:53.97 delay 1 second @12:18:54.98 AS.reconfigure ASfast @12:18:55.01 AS.seek 0.000ml @12:19:22.79 delay 3 seconds @12:19:25.80 AFV.select :carrierSolution @12:19:25.89 delay 2 seconds @12:19:27.90 AS.reconfigure ASconfig @12:19:27.93 AS.seek 1.000ml @12:20:26.51 delay 1 second @12:20:27.52 AFV.select :holdingCoil @12:20:27.73 delay 1 second @12:20:28.74 AS.reconfigure ASnorm @12:20:28.77 AS.seek 0.000ml @12:21:20.23 Setting up PCR for ENTap -- writing /var/log/esp/ENTap14mar1812h1ml.pcr @12:21:20.24 Building PCR Slug from ASV with MM1 mix, PP1 primer @12:21:20.25 Priming with carrier @12:21:20.26 ATV.dial ARV @12:21:21.29 ARV.dial :carrier @12:21:21.57 AS.seek 0.500ml @12:21:47.59 AFV.select :carrierSolution @12:21:47.69 delay 2 seconds @12:21:49.70 AS.reconfigure ASconfig @12:21:49.74 AS.seek 1.000ml @12:22:19.18 delay 1 second @12:22:20.19 AFV.select :holdingCoil @12:22:20.28 delay 1 second @12:22:21.29 ATV.dial :waste @12:22:22.65 AS.reconfigure ASnorm @12:22:22.69 AS.seek empty @12:23:14.10 AFV.select :carrierSolution @12:23:14.20 delay 2 seconds @12:23:16.21 AS.reconfigure ASconfig @12:23:16.24 AS.seek 0.500ml @12:23:45.78 delay 1 second @12:23:46.79 AFV.select :holdingCoil @12:23:46.88 Building Slug @12:23:46.89 ATV.dial :ARV @12:23:48.14 delay 2 seconds @12:23:50.15 ARV.dial :air1 @12:23:50.47 delay 2 seconds @12:23:52.53 AS.reconfigure ASslow2 @12:23:52.56 AS.seek 0.506ml @12:23:59.10 delay 2 seconds @12:24:01.11 ARV.dial :MM1 @12:24:01.61 delay 2 seconds @12:24:03.62 Adding MM1 @12:24:03.68 AS.seek 0.524ml @12:24:22.28 delay 25 seconds @12:24:47.29 ARV.dial :PP1 @12:24:47.61 Adding PP1 @12:24:47.63 delay 2 seconds @12:24:49.68 AS.seek 0.530ml @12:24:55.95 delay 10 seconds @12:25:05.96 ARV.dial :air1 @12:25:06.27 adding ARV air @12:25:06.28 delay 2 seconds @12:25:08.34 AS.seek 0.536ml @12:25:14.73 delay 5 seconds @12:25:19.74 ARV.dial :carrier @12:25:20.05 delay 7 seconds @12:25:27.11 AS.seek 0.594ml @12:26:26.65 delay 5 seconds @12:26:31.66 ATV.dial :ASV @12:26:32.08 delay 2 seconds @12:26:34.14 AS.seek 0.600ml @12:26:40.59 delay 4 seconds @12:26:44.60 ATV.dial ARV @12:26:44.90 delay 2 seconds @12:26:46.91 ARV.dial :carrier @12:26:46.92 delay 2 seconds @12:26:48.97 AS.seek 0.650ml @12:27:40.17 delay 4 seconds @12:27:44.18 ATV.dial PCR @12:27:44.79 ASV.dial :waste1 @12:27:44.80 ARV.dial :air1 @12:27:45.15 Positioning Slug by 0.324ml @12:27:45.20 AS.seek 0.326ml @12:33:15.86 delay 5 seconds @12:33:20.88 ATV.dial ARV @12:33:21.38 ASV.dial :PCRlock @12:33:21.81 delay 5 seconds @12:33:26.82 Running ENTap Profile60C thermocycling @12:33:26.83 PCR.program Profile60C @12:33:26.93 Profile60C should complete in < 1:39:08 @12:33:26.94 PCR.run 2:08:55 @13:56:25.31 PCR.off @13:56:25.83 PCR for ENTap completed -- closed /var/log/esp/ENTap14mar1812h1ml.pcr @13:56:25.88 Cleaning PCR @13:56:25.89 AFV.select :carrierSolution @13:56:25.99 delay 2 seconds @13:56:28.00 AS.reconfigure ASconfig @13:56:28.03 AS.seek 0.500ml @13:56:38.46 delay 1 second @13:56:39.47 AFV.select :holdingCoil @13:56:39.56 ASV.dial :waste1 @13:56:40.03 ATV.dial :air3 @13:56:40.97 AS.reconfigure ASslow1 @13:56:41.01 AS.seek 0.510ml @13:56:43.31 delay 2 seconds @13:56:45.32 ATV.dial :bleach @13:56:45.72 AS.seek 0.810ml @13:57:47.12 delay 3 seconds @13:57:50.13 ATV.dial :air3 @13:57:50.48 delay 3 seconds @13:57:53.54 AS.seek 0.835ml @13:57:58.88 delay 3 seconds @13:58:01.89 ATV.dial :bleach @13:58:02.37 AS.seek 0.820ml @13:58:05.68 delay 3 seconds @13:58:08.69 ARV.dial :waste @13:58:09.30 delay 3 seconds @13:58:12.31 ATV.dial :ARV @13:58:13.05 AS.reconfigure ASconfig @13:58:13.09 AS.seek 0.470ml @13:58:33.78 AFV.select :carrierSolution @13:58:33.88 delay 2 seconds @13:58:35.89 AS.seek 1.000ml @13:59:07.08 delay 1 second @13:59:08.09 AFV.select :holdingCoil @13:59:08.18 delay 1 second @13:59:09.19 AS.reconfigure ASfast @13:59:09.22 AS.seek 0.000ml @13:59:36.94 AFV.select :carrierSolution @13:59:37.04 delay 2 seconds @13:59:39.05 AS.reconfigure ASconfig @13:59:39.08 AS.seek 1.000ml @14:00:37.75 delay 1 second @14:00:38.76 AFV.select :holdingCoil @14:00:38.85 delay 1 second @14:00:39.86 AS.reconfigure ASfast @14:00:39.89 AS.seek 0.000ml @14:01:07.60 AFV.select :carrierSolution @14:01:07.70 delay 2 seconds @14:01:09.71 AS.reconfigure ASconfig @14:01:09.74 AS.seek 1.000ml @14:02:08.43 delay 1 second @14:02:09.44 AFV.select :holdingCoil @14:02:09.53 delay 1 second @14:02:10.54 AS.reconfigure ASfast @14:02:10.57 AS.seek 0.000ml @14:02:38.31 ARV.dial between carrier and air1 @14:02:38.82 ATV.dial :air3 @14:02:39.84 AS.reconfigure ASslow1 @14:02:39.87 AS.seek 0.010ml @14:02:42.25 delay 2 seconds @14:02:44.25 ATV.dial :bleach @14:02:44.73 AS.seek 0.810ml @14:05:28.14 delay 3 seconds @14:05:31.15 ATV.dial :air3 @14:05:31.47 delay 3 seconds @14:05:34.53 AS.seek 0.835ml @14:05:39.88 delay 3 seconds @14:05:42.89 ATV.dial :bleach @14:05:43.29 AS.seek 0.820ml @14:05:46.58 delay 3 seconds @14:05:49.59 ATV.dial PCR @14:05:49.92 AS.reconfigure ASconfig @14:05:49.95 AS.seek empty @14:06:38.08 PCR.program :bleachProfile @14:06:38.13 PCR program should complete in < 2:45 @14:06:38.14 PCR.run 10:00 @14:09:36.33 PCR.off @14:09:36.36 AFV.select :carrierSolution @14:09:36.45 delay 2 seconds @14:09:38.46 AS.seek 1.000ml @14:10:37.20 delay 1 second @14:10:38.21 AFV.select :holdingCoil @14:10:38.30 delay 1 second @14:10:39.31 AS.reconfigure ASfast @14:10:39.34 AS.seek 0.000ml @14:11:07.05 delay 3 seconds @14:11:10.06 AFV.select :carrierSolution @14:11:10.15 delay 2 seconds @14:11:12.16 AS.reconfigure ASconfig @14:11:12.19 AS.seek 1.000ml @14:12:10.85 delay 1 second @14:12:11.86 AFV.select :holdingCoil @14:12:11.95 delay 1 second @14:12:12.96 AS.reconfigure ASfast @14:12:12.99 AS.seek 0.000ml @14:12:40.71 delay 3 seconds @14:12:43.72 AFV.select :carrierSolution @14:12:43.81 delay 2 seconds @14:12:45.82 AS.reconfigure ASconfig @14:12:45.85 AS.seek 1.000ml @14:13:44.53 delay 1 second @14:13:45.54 AFV.select :holdingCoil @14:13:45.63 delay 1 second @14:13:46.64 AS.reconfigure ASfast @14:13:46.67 AS.seek 0.000ml @14:14:14.39 delay 3 seconds @14:14:17.40 AFV.select :carrierSolution @14:14:17.49 delay 2 seconds @14:14:20.02 AS.reconfigure ASconfig @14:14:20.05 AS.seek 1.000ml @14:15:18.73 delay 1 second @14:15:19.74 AFV.select :holdingCoil @14:15:19.83 delay 1 second @14:15:20.84 AS.reconfigure ASfast @14:15:20.87 AS.seek 0.000ml @14:15:48.59 delay 3 seconds @14:15:51.60 AFV.select :carrierSolution @14:15:51.69 delay 2 seconds @14:15:53.70 AS.reconfigure ASconfig @14:15:53.73 AS.seek 1.000ml @14:16:52.41 delay 1 second @14:16:53.42 AFV.select :holdingCoil @14:16:53.51 delay 1 second @14:16:54.52 AS.reconfigure ASnorm @14:16:54.55 AS.seek 0.000ml @14:17:46.00 Setting up PCR for ENTem -- writing /var/log/esp/ENTem14mar1814h1ml.pcr @14:17:46.01 Building PCR Slug from ASV with MM2 mix, PP3 primer @14:17:46.02 Priming with carrier @14:17:46.03 ATV.dial ARV @14:17:46.55 ARV.dial :carrier @14:17:46.82 AS.seek 0.500ml @14:18:12.77 AFV.select :carrierSolution @14:18:12.89 delay 2 seconds @14:18:14.90 AS.reconfigure ASconfig @14:18:14.94 AS.seek 1.000ml @14:18:44.38 delay 1 second @14:18:45.39 AFV.select :holdingCoil @14:18:45.48 delay 1 second @14:18:46.49 ATV.dial :waste @14:18:47.75 AS.reconfigure ASnorm @14:18:47.78 AS.seek empty @14:19:39.41 AFV.select :carrierSolution @14:19:39.51 delay 2 seconds @14:19:41.52 AS.reconfigure ASconfig @14:19:41.55 AS.seek 0.500ml @14:20:11.01 delay 1 second @14:20:12.02 AFV.select :holdingCoil @14:20:12.11 Building Slug @14:20:12.12 ATV.dial :ARV @14:20:13.37 delay 2 seconds @14:20:15.38 ARV.dial :air1 @14:20:15.70 delay 2 seconds @14:20:17.76 AS.reconfigure ASslow2 @14:20:17.79 AS.seek 0.506ml @14:20:24.14 delay 2 seconds @14:20:26.15 ARV.dial :MM2 @14:20:27.37 delay 2 seconds @14:20:29.38 Adding MM2 @14:20:29.44 AS.seek 0.524ml @14:20:47.92 delay 25 seconds @14:21:12.93 ARV.dial :PP3 @14:21:13.27 Adding PP3 @14:21:13.28 delay 2 seconds @14:21:15.33 AS.seek 0.530ml @14:21:21.59 delay 10 seconds @14:21:31.60 ARV.dial :air2 @14:21:32.04 adding ARV air @14:21:32.05 delay 2 seconds @14:21:34.10 AS.seek 0.536ml @14:21:40.62 delay 5 seconds @14:21:45.63 ARV.dial :carrier @14:21:46.65 delay 7 seconds @14:21:53.71 AS.seek 0.594ml @14:22:53.38 delay 5 seconds @14:22:58.39 ATV.dial :ASV @14:22:58.81 delay 2 seconds @14:23:00.87 AS.seek 0.600ml @14:23:07.34 delay 4 seconds @14:23:11.35 ATV.dial ARV @14:23:11.67 delay 2 seconds @14:23:13.68 ARV.dial :carrier @14:23:13.69 delay 2 seconds @14:23:15.74 AS.seek 0.650ml @14:24:06.84 delay 4 seconds @14:24:10.85 ATV.dial PCR @14:24:11.46 ASV.dial :waste1 @14:24:11.47 ARV.dial :air1 @14:24:11.81 Positioning Slug by 0.324ml @14:24:11.86 AS.seek 0.326ml @14:29:42.67 delay 5 seconds @14:29:47.68 ATV.dial ARV @14:29:48.18 ASV.dial :PCRlock @14:29:48.67 delay 5 seconds @14:29:53.68 Running ENTem ProfileEM60C thermocycling @14:29:53.69 PCR.program ProfileEM60C @14:29:53.80 ProfileEM60C should complete in < 2:11:38 @14:29:53.81 PCR.run 2:49:32.5 @16:26:03.61 PCR.off @16:26:04.15 PCR for ENTem completed -- closed /var/log/esp/ENTem14mar1814h1ml.pcr @16:26:04.20 Cleaning PCR @16:26:04.21 AFV.select :carrierSolution @16:26:04.30 delay 2 seconds @16:26:06.31 AS.reconfigure ASconfig @16:26:06.34 AS.seek 0.500ml @16:26:16.73 delay 1 second @16:26:17.74 AFV.select :holdingCoil @16:26:17.83 ASV.dial :waste1 @16:26:18.59 ATV.dial :air3 @16:26:19.54 AS.reconfigure ASslow1 @16:26:19.57 AS.seek 0.510ml @16:26:21.90 delay 2 seconds @16:26:23.91 ATV.dial :bleach @16:26:24.31 AS.seek 0.810ml @16:27:25.71 delay 3 seconds @16:27:28.72 ATV.dial :air3 @16:27:29.07 delay 3 seconds @16:27:32.13 AS.seek 0.835ml @16:27:37.50 delay 3 seconds @16:27:40.51 ATV.dial :bleach @16:27:40.90 AS.seek 0.820ml @16:27:44.22 delay 3 seconds @16:27:47.23 ARV.dial :waste @16:27:47.82 delay 3 seconds @16:27:50.83 ATV.dial :ARV @16:27:51.56 AS.reconfigure ASconfig @16:27:51.60 AS.seek 0.470ml @16:28:12.41 AFV.select :carrierSolution @16:28:12.51 delay 2 seconds @16:28:14.52 AS.seek 1.000ml @16:28:45.74 delay 1 second @16:28:46.75 AFV.select :holdingCoil @16:28:46.84 delay 1 second @16:28:47.85 AS.reconfigure ASfast @16:28:47.88 AS.seek 0.000ml @16:29:15.61 AFV.select :carrierSolution @16:29:15.71 delay 2 seconds @16:29:17.72 AS.reconfigure ASconfig @16:29:17.75 AS.seek 1.000ml @16:30:16.44 delay 1 second @16:30:17.45 AFV.select :holdingCoil @16:30:17.54 delay 1 second @16:30:18.55 AS.reconfigure ASfast @16:30:18.58 AS.seek 0.000ml @16:30:46.28 AFV.select :carrierSolution @16:30:46.38 delay 2 seconds @16:30:48.39 AS.reconfigure ASconfig @16:30:48.42 AS.seek 1.000ml @16:31:47.07 delay 1 second @16:31:48.08 AFV.select :holdingCoil @16:31:48.17 delay 1 second @16:31:49.18 AS.reconfigure ASfast @16:31:49.21 AS.seek 0.000ml @16:32:16.93 ARV.dial between carrier and air1 @16:32:17.34 ATV.dial :air3 @16:32:18.37 AS.reconfigure ASslow1 @16:32:18.40 AS.seek 0.010ml @16:32:20.79 delay 2 seconds @16:32:22.80 ATV.dial :bleach @16:32:23.29 AS.seek 0.810ml @16:35:06.69 delay 3 seconds @16:35:09.70 ATV.dial :air3 @16:35:10.03 delay 3 seconds @16:35:13.09 AS.seek 0.835ml @16:35:18.42 delay 3 seconds @16:35:21.43 ATV.dial :bleach @16:35:21.82 AS.seek 0.820ml @16:35:25.14 delay 3 seconds @16:35:28.15 ATV.dial PCR @16:35:28.48 AS.reconfigure ASconfig @16:35:28.51 AS.seek empty @16:36:16.61 PCR.program :bleachProfile @16:36:16.66 PCR program should complete in < 2:45 @16:36:16.67 PCR.run 10:00 @16:39:14.86 PCR.off @16:39:14.89 AFV.select :carrierSolution @16:39:14.98 delay 2 seconds @16:39:16.99 AS.seek 1.000ml @16:40:15.72 delay 1 second @16:40:16.73 AFV.select :holdingCoil @16:40:16.82 delay 1 second @16:40:18.40 AS.reconfigure ASfast @16:40:18.44 AS.seek 0.000ml @16:40:46.16 delay 3 seconds @16:40:49.17 AFV.select :carrierSolution @16:40:49.26 delay 2 seconds @16:40:51.27 AS.reconfigure ASconfig @16:40:51.30 AS.seek 1.000ml @16:41:49.99 delay 1 second @16:41:51.00 AFV.select :holdingCoil @16:41:51.09 delay 1 second @16:41:52.10 AS.reconfigure ASfast @16:41:52.14 AS.seek 0.000ml @16:42:19.88 delay 3 seconds @16:42:22.89 AFV.select :carrierSolution @16:42:22.98 delay 2 seconds @16:42:24.99 AS.reconfigure ASconfig @16:42:25.02 AS.seek 1.000ml @16:43:23.69 delay 1 second @16:43:24.70 AFV.select :holdingCoil @16:43:24.79 delay 1 second @16:43:25.80 AS.reconfigure ASfast @16:43:25.83 AS.seek 0.000ml @16:43:53.59 delay 3 seconds @16:43:56.60 AFV.select :carrierSolution @16:43:56.69 delay 2 seconds @16:43:58.70 AS.reconfigure ASconfig @16:43:58.73 AS.seek 1.000ml @16:44:57.36 delay 1 second @16:44:58.37 AFV.select :holdingCoil @16:44:58.46 delay 1 second @16:44:59.47 AS.reconfigure ASfast @16:44:59.50 AS.seek 0.000ml @16:45:27.21 delay 3 seconds @16:45:30.22 AFV.select :carrierSolution @16:45:30.31 delay 2 seconds @16:45:32.32 AS.reconfigure ASconfig @16:45:32.36 AS.seek 1.000ml @16:46:31.04 delay 1 second @16:46:32.05 AFV.select :holdingCoil @16:46:32.14 delay 1 second @16:46:33.15 AS.reconfigure ASnorm @16:46:33.18 AS.seek 0.000ml @16:47:24.62 Setting up PCR for IPCap -- writing /var/log/esp/IPCap14mar1816h1ml.pcr @16:47:24.63 Building PCR Slug from ASV with MM1 mix, PP2 primer @16:47:24.64 Priming with carrier @16:47:24.65 ATV.dial ARV @16:47:25.16 ARV.dial :carrier @16:47:25.45 AS.seek 0.500ml @16:47:51.37 AFV.select :carrierSolution @16:47:51.47 delay 2 seconds @16:47:53.48 AS.reconfigure ASconfig @16:47:53.52 AS.seek 1.000ml @16:48:22.99 delay 1 second @16:48:24.00 AFV.select :holdingCoil @16:48:24.09 delay 1 second @16:48:25.10 ATV.dial :waste @16:48:26.36 AS.reconfigure ASnorm @16:48:26.39 AS.seek empty @16:49:18.00 AFV.select :carrierSolution @16:49:18.10 delay 2 seconds @16:49:20.11 AS.reconfigure ASconfig @16:49:20.14 AS.seek 0.500ml @16:49:49.62 delay 1 second @16:49:50.63 AFV.select :holdingCoil @16:49:50.72 Building Slug @16:49:50.73 ATV.dial :ARV @16:49:51.98 delay 2 seconds @16:49:53.99 ARV.dial :air1 @16:49:54.31 delay 2 seconds @16:49:56.37 AS.reconfigure ASslow2 @16:49:56.40 AS.seek 0.506ml @16:50:02.75 delay 2 seconds @16:50:04.76 ARV.dial :MM1 @16:50:05.34 delay 2 seconds @16:50:07.35 Adding MM1 @16:50:07.41 AS.seek 0.524ml @16:50:25.92 delay 25 seconds @16:50:50.93 ARV.dial :PP2 @16:50:51.26 Adding PP2 @16:50:51.28 delay 2 seconds @16:50:53.33 AS.seek 0.530ml @16:50:59.57 delay 10 seconds @16:51:09.58 ARV.dial :air2 @16:51:09.98 adding ARV air @16:51:09.99 delay 2 seconds @16:51:12.05 AS.seek 0.536ml @16:51:18.54 delay 5 seconds @16:51:23.55 ARV.dial :carrier @16:51:24.59 delay 7 seconds @16:51:31.65 AS.seek 0.594ml @16:52:31.15 delay 5 seconds @16:52:36.16 ATV.dial :ASV @16:52:36.49 delay 2 seconds @16:52:38.55 AS.seek 0.600ml @16:52:44.83 delay 4 seconds @16:52:48.84 ATV.dial ARV @16:52:49.16 delay 2 seconds @16:52:51.17 ARV.dial :carrier @16:52:51.18 delay 2 seconds @16:52:53.23 AS.seek 0.650ml @16:53:44.44 delay 4 seconds @16:53:48.45 ATV.dial PCR @16:53:48.94 ASV.dial :waste1 @16:53:48.95 ARV.dial :air1 @16:53:49.30 Positioning Slug by 0.324ml @16:53:49.35 AS.seek 0.326ml @16:59:20.18 delay 5 seconds @16:59:25.19 ATV.dial ARV @16:59:25.78 ASV.dial :PCRlock @16:59:26.32 delay 5 seconds @16:59:31.33 Running IPCap Profile59C thermocycling @16:59:31.34 PCR.program Profile59C @16:59:31.44 Profile59C should complete in < 1:39:08 @16:59:31.45 PCR.run 2:08:55 @18:23:31.97 PCR.off @18:23:32.51 PCR for IPCap completed -- closed /var/log/esp/IPCap14mar1816h1ml.pcr @18:23:32.56 Cleaning PCR @18:23:32.57 AFV.select :carrierSolution @18:23:32.66 delay 2 seconds @18:23:34.67 AS.reconfigure ASconfig @18:23:34.70 AS.seek 0.500ml @18:23:45.10 delay 1 second @18:23:46.11 AFV.select :holdingCoil @18:23:46.20 ASV.dial :waste1 @18:23:46.58 ATV.dial :air3 @18:23:47.52 AS.reconfigure ASslow1 @18:23:47.56 AS.seek 0.510ml @18:23:49.75 delay 2 seconds @18:23:51.76 ATV.dial :bleach @18:23:52.24 AS.seek 0.810ml @18:24:53.66 delay 3 seconds @18:24:56.67 ATV.dial :air3 @18:24:57.00 delay 3 seconds @18:25:00.06 AS.seek 0.835ml @18:25:05.39 delay 3 seconds @18:25:08.40 ATV.dial :bleach @18:25:08.79 AS.seek 0.820ml @18:25:12.09 delay 3 seconds @18:25:15.10 ARV.dial :waste @18:25:15.59 delay 3 seconds @18:25:18.60 ATV.dial :ARV @18:25:19.32 AS.reconfigure ASconfig @18:25:19.36 AS.seek 0.470ml @18:25:40.05 AFV.select :carrierSolution @18:25:40.15 delay 2 seconds @18:25:42.16 AS.seek 1.000ml @18:26:13.39 delay 1 second @18:26:14.40 AFV.select :holdingCoil @18:26:14.49 delay 1 second @18:26:15.50 AS.reconfigure ASfast @18:26:15.53 AS.seek 0.000ml @18:26:43.26 AFV.select :carrierSolution @18:26:43.36 delay 2 seconds @18:26:45.37 AS.reconfigure ASconfig @18:26:45.40 AS.seek 1.000ml @18:27:44.09 delay 1 second @18:27:45.10 AFV.select :holdingCoil @18:27:45.19 delay 1 second @18:27:46.20 AS.reconfigure ASfast @18:27:46.23 AS.seek 0.000ml @18:28:13.90 AFV.select :carrierSolution @18:28:14.00 delay 2 seconds @18:28:16.01 AS.reconfigure ASconfig @18:28:16.04 AS.seek 1.000ml @18:29:14.68 delay 1 second @18:29:15.69 AFV.select :holdingCoil @18:29:15.78 delay 1 second @18:29:16.79 AS.reconfigure ASfast @18:29:16.82 AS.seek 0.000ml @18:29:44.54 ARV.dial between carrier and air1 @18:29:44.95 ATV.dial :air3 @18:29:45.89 AS.reconfigure ASslow1 @18:29:45.93 AS.seek 0.010ml @18:29:48.37 delay 2 seconds @18:29:50.38 ATV.dial :bleach @18:29:50.78 AS.seek 0.810ml @18:32:34.15 delay 3 seconds @18:32:37.16 ATV.dial :air3 @18:32:37.50 delay 3 seconds @18:32:40.56 AS.seek 0.835ml @18:32:45.90 delay 3 seconds @18:32:48.91 ATV.dial :bleach @18:32:49.30 AS.seek 0.820ml @18:32:52.61 delay 3 seconds @18:32:56.19 ATV.dial PCR @18:32:56.52 AS.reconfigure ASconfig @18:32:56.55 AS.seek empty @18:33:44.68 PCR.program :bleachProfile @18:33:44.73 PCR program should complete in < 2:45 @18:33:44.74 PCR.run 10:00 @18:36:45.95 PCR.off @18:36:45.98 AFV.select :carrierSolution @18:36:46.07 delay 2 seconds @18:36:48.08 AS.seek 1.000ml @18:37:46.81 delay 1 second @18:37:47.82 AFV.select :holdingCoil @18:37:47.91 delay 1 second @18:37:48.92 AS.reconfigure ASfast @18:37:48.95 AS.seek 0.000ml @18:38:16.69 delay 3 seconds @18:38:19.70 AFV.select :carrierSolution @18:38:19.79 delay 2 seconds @18:38:21.80 AS.reconfigure ASconfig @18:38:21.83 AS.seek 1.000ml @18:39:20.50 delay 1 second @18:39:21.51 AFV.select :holdingCoil @18:39:21.60 delay 1 second @18:39:22.61 AS.reconfigure ASfast @18:39:22.65 AS.seek 0.000ml @18:39:50.36 delay 3 seconds @18:39:53.37 AFV.select :carrierSolution @18:39:53.46 delay 2 seconds @18:39:55.47 AS.reconfigure ASconfig @18:39:55.50 AS.seek 1.000ml @18:40:54.15 delay 1 second @18:40:55.16 AFV.select :holdingCoil @18:40:55.25 delay 1 second @18:40:56.26 AS.reconfigure ASfast @18:40:56.29 AS.seek 0.000ml @18:41:24.01 delay 3 seconds @18:41:27.02 AFV.select :carrierSolution @18:41:27.11 delay 2 seconds @18:41:29.12 AS.reconfigure ASconfig @18:41:29.15 AS.seek 1.000ml @18:42:27.79 delay 1 second @18:42:28.80 AFV.select :holdingCoil @18:42:28.89 delay 1 second @18:42:29.90 AS.reconfigure ASfast @18:42:29.94 AS.seek 0.000ml @18:42:57.68 delay 3 seconds @18:43:00.69 AFV.select :carrierSolution @18:43:00.78 delay 2 seconds @18:43:02.79 AS.reconfigure ASconfig @18:43:02.82 AS.seek 1.000ml @18:44:01.47 delay 1 second @18:44:02.48 AFV.select :holdingCoil @18:44:02.57 delay 1 second @18:44:03.58 AS.reconfigure ASnorm @18:44:03.61 AS.seek 0.000ml @18:44:55.00 Setting up PCR for IPCem -- writing /var/log/esp/IPCem14mar1818h1ml.pcr @18:44:55.01 Building PCR Slug from ASV with MM3 mix, PP5 primer @18:44:55.02 Priming with carrier @18:44:55.03 ATV.dial ARV @18:44:55.55 ARV.dial :carrier @18:44:55.84 AS.seek 0.500ml @18:45:21.80 AFV.select :carrierSolution @18:45:21.90 delay 2 seconds @18:45:23.91 AS.reconfigure ASconfig @18:45:23.94 AS.seek 1.000ml @18:45:53.38 delay 1 second @18:45:54.39 AFV.select :holdingCoil @18:45:54.48 delay 1 second @18:45:55.49 ATV.dial :waste @18:45:56.75 AS.reconfigure ASnorm @18:45:56.78 AS.seek empty @18:46:48.19 AFV.select :carrierSolution @18:46:48.29 delay 2 seconds @18:46:50.30 AS.reconfigure ASconfig @18:46:50.33 AS.seek 0.500ml @18:47:19.87 delay 1 second @18:47:20.88 AFV.select :holdingCoil @18:47:20.97 Building Slug @18:47:20.98 ATV.dial :ARV @18:47:22.23 delay 2 seconds @18:47:24.24 ARV.dial :air1 @18:47:24.56 delay 2 seconds @18:47:26.62 AS.reconfigure ASslow2 @18:47:26.65 AS.seek 0.506ml @18:47:32.92 delay 2 seconds @18:47:34.93 ARV.dial :MM3 @18:47:35.78 delay 2 seconds @18:47:37.79 Adding MM3 @18:47:37.84 AS.seek 0.524ml @18:47:56.45 delay 25 seconds @18:48:21.46 ARV.dial :PP5 @18:48:21.89 Adding PP5 @18:48:21.90 delay 2 seconds @18:48:23.97 AS.seek 0.530ml @18:48:30.43 delay 10 seconds @18:48:40.44 ARV.dial :air3 @18:48:40.77 adding ARV air @18:48:40.78 delay 2 seconds @18:48:42.84 AS.seek 0.536ml @18:48:49.18 delay 5 seconds @18:48:54.19 ARV.dial :carrier @18:48:55.23 delay 7 seconds @18:49:02.29 AS.seek 0.594ml @18:50:01.77 delay 5 seconds @18:50:06.78 ATV.dial :ASV @18:50:07.19 delay 2 seconds @18:50:09.25 AS.seek 0.600ml @18:50:15.55 delay 4 seconds @18:50:19.56 ATV.dial ARV @18:50:19.89 delay 2 seconds @18:50:21.90 ARV.dial :carrier @18:50:21.91 delay 2 seconds @18:50:23.96 AS.seek 0.650ml @18:51:15.16 delay 4 seconds @18:51:19.17 ATV.dial PCR @18:51:19.67 ASV.dial :waste1 @18:51:19.68 ARV.dial :air1 @18:51:20.03 Positioning Slug by 0.324ml @18:51:20.08 AS.seek 0.326ml @18:56:50.55 delay 5 seconds @18:56:55.56 ATV.dial ARV @18:56:56.07 ASV.dial :PCRlock @18:56:57.08 delay 5 seconds @18:57:02.09 Running IPCem ProfileEM59C thermocycling @18:57:02.10 PCR.program ProfileEM59C @18:57:02.21 ProfileEM59C should complete in < 2:11:38 @18:57:02.22 PCR.run 2:49:32.5 @20:53:13.83 PCR.off @20:53:14.37 PCR for IPCem completed -- closed /var/log/esp/IPCem14mar1818h1ml.pcr @20:53:14.38 Full MFB Decontamination Decon extraction column and decon AIV @20:53:14.40 SPE.hold 2:46:40,55.0C @20:53:14.42 ASV.dial :SPEdelivery @20:53:15.37 delay 20 seconds @20:53:35.38 AFV.select :carrierSolution @20:53:35.47 delay 2 seconds @20:53:37.48 AS.reconfigure ASconfig @20:53:37.52 AS.seek 0.500ml @20:53:47.95 delay 1 second @20:53:48.96 AFV.select :holdingCoil @20:53:49.05 ATV.dial :air1 @20:53:50.67 AS.reconfigure ASslow1 @20:53:50.70 AS.seek 0.520ml @20:53:55.03 ATV.dial :ASV @20:53:56.31 ASV.dial :SPErecovery @20:53:57.03 AS.seek 0.620ml @20:54:17.65 ATV.dial :waste @20:54:19.11 AS.reconfigure ASnorm @20:54:19.14 AS.seek empty @20:54:51.30 AFV.select :holdingCoil @20:54:51.31 ATV.dial :waste @20:54:51.32 AS.reconfigure ASconfig @20:54:51.35 AS.seek 0.000ml @20:54:51.66 AFV.select :carrierSolution @20:54:51.75 delay 2 seconds @20:54:53.76 AS.seek 0.500ml @20:55:23.18 delay 1 second @20:55:24.19 AFV.select :holdingCoil @20:55:24.28 ATV.dial :air3 @20:55:24.84 AS.reconfigure ASslow1 @20:55:24.88 AS.seek 0.520ml @20:55:29.19 ATV.dial :air3 @20:55:29.25 AS.seek 0.530ml @20:55:31.52 delay 2 seconds @20:55:33.53 ATV.dial :bleach @20:55:33.90 AS.seek 0.710ml @20:56:10.86 delay 3 seconds @20:56:13.87 ATV.dial :air3 @20:56:14.21 delay 3 seconds @20:56:17.27 AS.seek 0.735ml @20:56:22.55 delay 3 seconds @20:56:25.56 ATV.dial :bleach @20:56:25.95 AS.seek 0.720ml @20:56:29.24 delay 3 seconds @20:56:32.25 ATV.dial ASV @20:56:33.22 ASV.dial :SPErecovery @20:56:33.28 AS.seek 0.365ml @20:57:45.91 ATV.dial :waste @20:57:47.39 AS.reconfigure ASnorm @20:57:47.42 AS.seek empty @20:58:06.44 ATV.dial :air3 @20:58:07.08 AS.reconfigure ASslow1 @20:58:07.11 AS.seek 0.009ml @20:58:09.50 delay 2 seconds @20:58:11.51 ATV.dial :bleach @20:58:11.88 AS.seek 0.579ml @21:00:08.33 delay 3 seconds @21:00:11.34 ATV.dial :air3 @21:00:11.76 delay 3 seconds @21:00:14.82 AS.seek 0.604ml @21:00:20.15 delay 3 seconds @21:00:23.16 ATV.dial :bleach @21:00:23.53 AS.seek 0.589ml @21:00:26.81 delay 3 seconds @21:00:29.82 ATV.dial :ASV @21:00:30.72 ASV.dial :wasteR @21:00:31.49 AS.seek 0.000ml @21:02:31.95 delay 1:00 @21:03:31.96 ATV.dial ASV @21:03:31.97 AFV.select :carrierSolution @21:03:32.06 delay 2 seconds @21:03:34.07 AS.reconfigure ASconfig @21:03:34.11 AS.seek 1.000ml @21:04:32.76 delay 1 second @21:04:33.77 AFV.select :holdingCoil @21:04:33.86 delay 1 second @21:04:34.87 ASV.dial :wasteR @21:04:34.88 AS.reconfigure ASslow1 @21:04:34.91 AS.seek 0.000ml @21:07:59.43 delay 10 seconds @21:08:09.44 ATV.dial :air3 @21:08:10.57 AS.seek 0.010ml @21:08:12.98 delay 2 seconds @21:08:14.99 ATV.dial :bleach @21:08:15.39 AS.seek 0.580ml @21:10:11.89 delay 3 seconds @21:10:14.90 ATV.dial :air3 @21:10:15.24 delay 3 seconds @21:10:18.30 AS.seek 0.605ml @21:10:23.64 delay 3 seconds @21:10:26.65 ATV.dial :bleach @21:10:27.13 AS.seek 0.590ml @21:10:30.41 delay 3 seconds @21:10:33.42 ATV.dial ASV @21:10:34.32 ASV.dial :SPEdelivery @21:10:34.81 AS.seek 0.000ml @21:12:35.61 delay 1:00 @21:13:35.62 AFV.select :carrierSolution @21:13:35.71 delay 2 seconds @21:13:37.72 AS.reconfigure ASconfig @21:13:37.76 AS.seek 1.000ml @21:14:36.36 delay 1 second @21:14:37.37 AFV.select :holdingCoil @21:14:37.46 delay 1 second @21:14:38.47 ATV.dial ASV @21:14:38.48 ASV.dial :SPEdelivery @21:14:38.49 AS.reconfigure ASslow1 @21:14:38.52 AS.seek 0.000ml @21:18:02.99 delay 10 seconds @21:18:13.00 ATV.dial :air2 @21:18:13.73 AS.reconfigure ASslow0 @21:18:13.76 AS.seek 0.800ml @21:19:35.69 delay 3 seconds @21:19:38.70 ATV.dial :ASV @21:19:39.42 ASV.dial :SPEdelivery @21:19:39.44 AS.reconfigure ASslow1 @21:19:39.47 AS.seek 0.030ml @21:22:16.77 delay 20 seconds @21:22:36.78 ATV.dial :waste,via => air3 @21:22:38.24 AS.reconfigure ASconfig @21:22:38.27 AS.seek empty @21:22:40.33 SPE.coast @21:22:40.41 Removing Bleach from Mixing Coil @21:22:40.42 AFV.select :carrierSolution @21:22:40.51 delay 2 seconds @21:22:42.52 AS.seek 0.500ml @21:23:12.07 delay 1 second @21:23:13.08 AFV.select :holdingCoil @21:23:13.17 ATV.dial :ASV @21:23:14.63 ASV.dial :SPErecovery @21:23:15.31 AS.reconfigure ASslow1 @21:23:15.35 AS.seek 0.730ml @21:24:02.49 ASV.dial :wasteD @21:24:03.00 AS.reconfigure ASnorm @21:24:03.03 AS.seek 0.000ml @21:24:40.64 AFV.select :carrierSolution @21:24:40.74 delay 2 seconds @21:24:42.75 AS.reconfigure ASconfig @21:24:42.78 AS.seek 0.500ml @21:25:12.27 delay 1 second @21:25:13.28 AFV.select :holdingCoil @21:25:13.37 ATV.dial ASV @21:25:13.38 ASV.dial :SPErecovery @21:25:14.07 AS.reconfigure ASslow0 @21:25:14.11 AS.seek 0.300ml @21:25:34.81 ATV.dial :air2 @21:25:35.56 AS.reconfigure ASslow1 @21:25:35.60 AS.seek 0.320ml @21:25:39.95 ATV.dial ASV @21:25:40.71 AS.reconfigure ASslow0 @21:25:40.74 AS.seek 0.570ml @21:26:06.47 ASV.dial :wasteD @21:26:07.13 AS.reconfigure ASnorm @21:26:07.16 AS.seek 0.000ml @21:26:36.62 AFV.select :carrierSolution @21:26:36.72 delay 2 seconds @21:26:38.73 AS.reconfigure ASconfig @21:26:38.76 AS.seek 0.500ml @21:27:08.25 delay 1 second @21:27:09.26 AFV.select :holdingCoil @21:27:09.35 ATV.dial ASV @21:27:09.36 ASV.dial :SPErecovery @21:27:10.05 AS.reconfigure ASslow0 @21:27:10.09 AS.seek 0.300ml @21:27:30.76 ATV.dial :air2 @21:27:31.51 AS.reconfigure ASslow1 @21:27:31.55 AS.seek 0.320ml @21:27:35.93 ATV.dial ASV @21:27:37.20 AS.reconfigure ASslow0 @21:27:37.23 AS.seek 0.570ml @21:28:03.03 ASV.dial :wasteD @21:28:03.69 AS.reconfigure ASnorm @21:28:03.72 AS.seek 0.000ml @21:28:33.35 ATV.dial :air2 @21:28:34.07 AS.reconfigure ASslow0 @21:28:34.10 AS.seek 0.300ml @21:29:04.99 ATV.dial :ASV @21:29:05.70 ASV.dial :wasteD @21:29:05.72 AS.reconfigure ASconfig @21:29:05.75 AS.seek 0.050ml @21:29:20.63 ATV.dial :waste @21:29:22.10 AS.seek 0.000ml @21:29:25.29 ASV.dial :SPEdelivery @21:29:26.57 deconLysateINT @21:29:26.58 ATV.dial :air3 @21:29:27.23 AS.reconfigure ASslow1 @21:29:27.26 AS.seek 0.010ml @21:29:29.66 delay 2 seconds @21:29:31.67 ATV.dial :bleach @21:29:32.04 AS.seek 0.960ml @21:32:46.02 delay 3 seconds @21:32:49.03 ATV.dial :air3 @21:32:49.36 delay 3 seconds @21:32:52.42 AS.seek 0.985ml @21:32:57.75 delay 3 seconds @21:33:00.76 ATV.dial :bleach @21:33:01.15 AS.seek 0.970ml @21:33:04.50 delay 3 seconds @21:33:07.51 ATV.dial :AIV @21:33:08.67 AIV.dial :coilToMFB @21:33:08.68 AWV.select :waste @21:33:08.69 AFV.select :carrierSolution @21:33:08.78 delay 2 seconds @21:33:10.79 AS.reconfigure ASconfig @21:33:10.83 AS.seek 1.000ml @21:33:12.92 delay 1 second @21:33:13.93 AFV.select :holdingCoil @21:33:14.02 delay 1 second @21:33:15.03 AS.reconfigure ASslow0 @21:33:15.06 AS.seek empty @21:34:57.42 AFV.select :carrierSolution @21:34:57.52 delay 2 seconds @21:34:59.53 AS.reconfigure ASconfig @21:34:59.56 AS.seek 1.000ml @21:35:58.25 delay 1 second @21:35:59.26 AFV.select :holdingCoil @21:35:59.35 delay 1 second @21:36:00.36 AS.reconfigure ASslow0 @21:36:00.39 AS.seek empty @21:37:42.95 AFV.select :carrierSolution @21:37:43.05 delay 2 seconds @21:37:45.06 AS.reconfigure ASconfig @21:37:45.09 AS.seek 1.000ml @21:38:43.74 delay 1 second @21:38:44.75 AFV.select :holdingCoil @21:38:44.84 delay 1 second @21:38:45.85 AS.reconfigure ASslow0 @21:38:45.88 AS.seek empty @21:40:28.26 ATV.dial :air2 @21:40:28.84 AS.reconfigure ASslow1 @21:40:28.87 AS.seek 0.009ml @21:40:31.28 AS.reconfigure ASslow0 @21:40:31.32 AS.seek 1.000ml @21:42:12.64 ATV.dial AIV @21:42:13.29 AS.seek 0.020ml @21:43:53.54 ATV.dial :waste @21:43:54.81 AS.seek 0.000ml @21:43:57.13 AWV.select :air @21:43:57.28 Cleaning PCR @21:43:57.29 AFV.select :carrierSolution @21:43:57.38 delay 2 seconds @21:43:59.39 AS.reconfigure ASconfig @21:43:59.42 AS.seek 0.500ml @21:44:28.93 delay 1 second @21:44:29.94 AFV.select :holdingCoil @21:44:30.03 ASV.dial :waste1 @21:44:30.52 ATV.dial :air3 @21:44:31.08 AS.reconfigure ASslow1 @21:44:31.12 AS.seek 0.510ml @21:44:33.33 delay 2 seconds @21:44:35.34 ATV.dial :bleach @21:44:35.71 AS.seek 0.810ml @21:45:37.14 delay 3 seconds @21:45:40.15 ATV.dial :air3 @21:45:40.49 delay 3 seconds @21:45:43.55 AS.seek 0.835ml @21:45:48.88 delay 3 seconds @21:45:51.89 ATV.dial :bleach @21:45:52.29 AS.seek 0.820ml @21:45:55.61 delay 3 seconds @21:45:58.62 ARV.dial :waste @21:45:59.11 delay 3 seconds @21:46:02.12 ATV.dial :ARV @21:46:02.93 AS.reconfigure ASconfig @21:46:02.97 AS.seek 0.470ml @21:46:23.64 AFV.select :carrierSolution @21:46:23.73 delay 2 seconds @21:46:25.74 AS.seek 1.000ml @21:46:56.91 delay 1 second @21:46:57.92 AFV.select :holdingCoil @21:46:58.01 delay 1 second @21:46:59.02 AS.reconfigure ASfast @21:46:59.05 AS.seek 0.000ml @21:47:26.77 AFV.select :carrierSolution @21:47:26.87 delay 2 seconds @21:47:28.88 AS.reconfigure ASconfig @21:47:28.91 AS.seek 1.000ml @21:48:27.59 delay 1 second @21:48:28.60 AFV.select :holdingCoil @21:48:28.69 delay 1 second @21:48:29.70 AS.reconfigure ASfast @21:48:29.73 AS.seek 0.000ml @21:48:57.47 AFV.select :carrierSolution @21:48:57.57 delay 2 seconds @21:48:59.58 AS.reconfigure ASconfig @21:48:59.61 AS.seek 1.000ml @21:49:58.25 delay 1 second @21:49:59.26 AFV.select :holdingCoil @21:49:59.35 delay 1 second @21:50:00.36 AS.reconfigure ASfast @21:50:00.39 AS.seek 0.000ml @21:50:28.09 ARV.dial between carrier and air1 @21:50:28.49 ATV.dial :air3 @21:50:29.43 AS.reconfigure ASslow1 @21:50:30.00 AS.seek 0.010ml @21:50:32.43 delay 2 seconds @21:50:34.44 ATV.dial :bleach @21:50:34.92 AS.seek 0.810ml @21:53:18.32 delay 3 seconds @21:53:21.33 ATV.dial :air3 @21:53:21.66 delay 3 seconds @21:53:24.72 AS.seek 0.835ml @21:53:30.07 delay 3 seconds @21:53:33.08 ATV.dial :bleach @21:53:33.48 AS.seek 0.820ml @21:53:36.79 delay 3 seconds @21:53:39.80 ATV.dial PCR @21:53:40.12 AS.reconfigure ASconfig @21:53:40.15 AS.seek empty @21:54:28.28 PCR.program :bleachProfile @21:54:28.34 PCR program should complete in < 2:45 @21:54:28.35 PCR.run 10:00 @21:57:32.59 PCR.off @21:57:32.62 AFV.select :carrierSolution @21:57:32.71 delay 2 seconds @21:57:34.72 AS.seek 1.000ml @21:58:33.46 delay 1 second @21:58:34.47 AFV.select :holdingCoil @21:58:34.56 delay 1 second @21:58:35.57 AS.reconfigure ASfast @21:58:35.60 AS.seek 0.000ml @21:59:03.32 delay 3 seconds @21:59:06.33 AFV.select :carrierSolution @21:59:06.42 delay 2 seconds @21:59:08.43 AS.reconfigure ASconfig @21:59:08.46 AS.seek 1.000ml @22:00:07.14 delay 1 second @22:00:08.15 AFV.select :holdingCoil @22:00:08.24 delay 1 second @22:00:09.25 AS.reconfigure ASfast @22:00:09.28 AS.seek 0.000ml @22:00:37.00 delay 3 seconds @22:00:40.01 AFV.select :carrierSolution @22:00:40.10 delay 2 seconds @22:00:42.11 AS.reconfigure ASconfig @22:00:42.14 AS.seek 1.000ml @22:01:40.83 delay 1 second @22:01:41.84 AFV.select :holdingCoil @22:01:41.93 delay 1 second @22:01:42.94 AS.reconfigure ASfast @22:01:43.01 AS.seek 0.000ml @22:02:10.84 delay 3 seconds @22:02:13.85 AFV.select :carrierSolution @22:02:13.94 delay 2 seconds @22:02:15.95 AS.reconfigure ASconfig @22:02:15.98 AS.seek 1.000ml @22:03:14.61 delay 1 second @22:03:15.62 AFV.select :holdingCoil @22:03:15.71 delay 1 second @22:03:16.72 AS.reconfigure ASfast @22:03:16.75 AS.seek 0.000ml @22:03:44.48 delay 3 seconds @22:03:47.49 AFV.select :carrierSolution @22:03:47.58 delay 2 seconds @22:03:49.59 AS.reconfigure ASconfig @22:03:49.62 AS.seek 1.000ml @22:04:48.27 delay 1 second @22:04:49.28 AFV.select :holdingCoil @22:04:49.37 delay 1 second @22:04:50.38 AS.reconfigure ASnorm @22:04:50.41 AS.seek 0.000ml @22:05:41.81 AFV.select :carrierSolution @22:05:41.90 delay 2 seconds @22:05:43.91 AS.reconfigure ASconfig @22:05:43.94 AS.seek 1.000ml @22:06:42.61 delay 1 second @22:06:43.62 AFV.select :holdingCoil @22:06:43.71 delay 1 second @22:06:44.72 ATV.dial :waste @22:06:45.62 AS.reconfigure ASnorm @22:06:45.65 AS.seek 0.500ml @22:07:11.57 ATV.dial :waste2 @22:07:12.67 AS.seek 0.000ml @22:07:38.58
Cleaning PCR AFV.select :carrierSolution @22:07:38.67 delay 2 seconds @22:07:40.68 AS.reconfigure ASconfig @22:07:40.71 AS.seek 0.500ml @22:08:10.21 delay 1 second @22:08:11.22 AFV.select :holdingCoil @22:08:11.31 ASV.dial :waste1 @22:08:11.32 ATV.dial :air3 @22:08:12.80 AS.reconfigure ASslow1 @22:08:12.83 AS.seek 0.510ml @22:08:15.11 delay 2 seconds @22:08:17.12 ATV.dial :bleach @22:08:17.49 AS.seek 0.810ml @22:09:18.91 delay 3 seconds @22:09:21.92 ATV.dial :air3 @22:09:22.25 delay 3 seconds @22:09:25.31 AS.seek 0.835ml @22:09:30.66 delay 3 seconds @22:09:33.67 ATV.dial :bleach @22:09:34.16 AS.seek 0.820ml @22:09:37.47 delay 3 seconds @22:09:40.48 ARV.dial :waste @22:09:40.89 delay 3 seconds @22:09:43.90 ATV.dial :ARV @22:09:44.65 AS.reconfigure ASconfig @22:09:44.69 AS.seek 0.470ml @22:10:05.37 AFV.select :carrierSolution @22:10:05.46 delay 2 seconds @22:10:07.47 AS.seek 1.000ml @22:10:38.65 delay 1 second @22:10:39.66 AFV.select :holdingCoil @22:10:39.75 delay 1 second @22:10:40.76 AS.reconfigure ASfast @22:10:40.79 AS.seek 0.000ml @22:11:08.48 AFV.select :carrierSolution @22:11:08.57 delay 2 seconds @22:11:10.58 AS.reconfigure ASconfig @22:11:10.61 AS.seek 1.000ml @22:12:09.28 delay 1 second @22:12:10.29 AFV.select :holdingCoil @22:12:10.38 delay 1 second @22:12:11.39 AS.reconfigure ASfast @22:12:11.42 AS.seek 0.000ml @22:12:39.13 AFV.select :carrierSolution @22:12:39.22 delay 2 seconds @22:12:41.23 AS.reconfigure ASconfig @22:12:41.26 AS.seek 1.000ml @22:13:39.89 delay 1 second @22:13:40.90 AFV.select :holdingCoil @22:13:40.99 delay 1 second @22:13:42.50 AS.reconfigure ASfast @22:13:42.54 AS.seek 0.000ml @22:14:10.27 ARV.dial between carrier and air1 @22:14:10.69 ATV.dial :air3 @22:14:11.63 AS.reconfigure ASslow1 @22:14:11.66 AS.seek 0.010ml @22:14:14.09 delay 2 seconds @22:14:16.10 ATV.dial :bleach @22:14:16.49 AS.seek 0.810ml @22:16:59.95 delay 3 seconds @22:17:02.97 ATV.dial :air3 @22:17:03.34 delay 3 seconds @22:17:06.40 AS.seek 0.835ml @22:17:11.73 delay 3 seconds @22:17:14.75 ATV.dial :bleach @22:17:15.14 AS.seek 0.820ml @22:17:18.43 delay 3 seconds @22:17:21.45 ATV.dial PCR @22:17:21.77 AS.reconfigure ASconfig @22:17:21.80 AS.seek empty @22:18:10.06 PCR.program :bleachProfile @22:18:10.12 PCR program should complete in < 2:45 @22:18:10.13 PCR.run 10:00 @22:21:14.36 PCR.off @22:21:14.39 AFV.select :carrierSolution @22:21:14.48 delay 2 seconds @22:21:16.49 AS.seek 1.000ml @22:22:15.13 delay 1 second @22:22:16.15 AFV.select :holdingCoil @22:22:16.24 delay 1 second @22:22:17.25 AS.reconfigure ASfast @22:22:17.28 AS.seek 0.000ml @22:22:45.09 delay 3 seconds @22:22:48.10 AFV.select :carrierSolution @22:22:48.19 delay 2 seconds @22:22:50.20 AS.reconfigure ASconfig @22:22:50.23 AS.seek 1.000ml @22:23:48.86 delay 1 second @22:23:49.88 AFV.select :holdingCoil @22:23:49.97 delay 1 second @22:23:50.98 AS.reconfigure ASfast @22:23:51.01 AS.seek 0.000ml @22:24:18.75 delay 3 seconds @22:24:21.77 AFV.select :carrierSolution @22:24:21.86 delay 2 seconds @22:24:23.87 AS.reconfigure ASconfig @22:24:23.90 AS.seek 1.000ml @22:25:22.57 delay 1 second @22:25:23.59 AFV.select :holdingCoil @22:25:23.68 delay 1 second @22:25:24.69 AS.reconfigure ASfast @22:25:24.72 AS.seek 0.000ml @22:25:52.43 delay 3 seconds @22:25:55.45 AFV.select :carrierSolution @22:25:55.54 delay 2 seconds @22:25:57.55 AS.reconfigure ASconfig @22:25:57.59 AS.seek 1.000ml @22:26:56.23 delay 1 second @22:26:57.24 AFV.select :holdingCoil @22:26:57.33 delay 1 second @22:26:58.34 AS.reconfigure ASfast @22:26:58.37 AS.seek 0.000ml @22:27:26.09 delay 3 seconds @22:27:29.11 AFV.select :carrierSolution @22:27:29.20 delay 2 seconds @22:27:31.21 AS.reconfigure ASconfig @22:27:31.24 AS.seek 1.000ml @22:28:29.85 delay 1 second @22:28:30.86 AFV.select :holdingCoil @22:28:30.95 delay 1 second @22:28:31.96 AS.reconfigure ASnorm @22:28:31.99 AS.seek 0.000ml @22:29:23.43 Completed {wqPCR} begun 08:01:05 @22:29:23.46 Asleep til 08:01PDT19-Mar-14 @22:29:23.47 AS.stop @22:29:23.48
Sleeping until 08:01PDT19-Mar-14 @22:29:23.50 GoodNews.email "Then, in tube 3: wqPCR\n/home/esp/esp2/mission/14mar14sccwrp.rb:68",Subject => "Asleep until 08:01PDT19-Mar-14" @22:29:25.02 CC.stop @22:29:25.05 CS.stop @22:29:25.07 PC.stop @22:29:25.10 PS.stop @22:29:25.13 Forearm.stop @22:29:25.16 Elbow.stop @22:29:25.19 SS.stop @22:29:25.26 Sleepy.power :raw,:OFF @22:29:25.27 Sleepy.power :dwarf,:OFF @22:29:25.28 Shutdown @08:01:05.69PDT19-Mar-14
Powering up Dwarves @08:01:10.65 Sleepy.power :raw,:ON @08:01:11.12 SPE.configure SPEconfig @08:01:11.14 AS.configure ASconfig @08:01:11.18 CC.configure CCconfig @08:01:11.21 CS.configure CSconfig @08:01:11.24 PC.configure PCconfig @08:01:11.27 PS.configure PSconfig @08:01:11.30 Forearm.configure LooseForearm @08:01:11.33 Elbow.configure LooseElbow @08:01:11.36 SC.configure SCloose @08:01:11.39 SE.configure SEconfig @08:01:11.42 SS.configure SSconfig @08:01:11.45 CH.configure CHconfig @08:01:11.47 PH.configure PHconfig @08:01:11.50 Valve.reset @08:01:12.15 PCR.off @08:01:12.18 PCR.pidtemp= 50,0.1,0,300,25,25 @08:01:12.21 PCR.caltemp= 6.6661,0.0344,0.0 @08:01:12.31 Intake.select :close @08:01:12.33 Exhaust.select :close @08:01:12.34 CTV.dial :air @08:01:12.38 PSV.dial :air @08:01:12.42
PC.reconfigure PCconfig @08:01:12.47 PC.home.seek open @08:01:12.49 CSV.dial :waste1 @08:01:13.00 PS.reconfigure PSconfig @08:01:13.04 PS.home.seek 0.000ml @08:01:13.06 SSV.dial :spit,avoiding => exhaust @08:01:13.88 CS.reconfigure CSfast @08:01:13.91 SS.reconfigure SSconfig @08:01:14.00 SS.home.seek 0.00ml @08:01:14.02 CS.home.seek 0.000ml @08:01:14.10 CC.reconfigure CCconfig @08:01:14.13 CC.home.seek open @08:01:23.30 PS.coast @08:01:23.38
CC.seek open @08:01:23.40 PC.seek open @08:01:23.54
Forearm.reconfigure LooseForearm @08:01:23.58 Forearm.home.seek retract @08:01:24.31 Hand.grip @08:01:24.33
Elbow.jog -1500 @08:01:24.89 Elbow.reconfigure LooseElbow @08:01:24.93 Elbow.home.seek clear @08:01:29.68 SE.reconfigure SEconfig @08:01:29.71 SE.home.seek down @08:01:30.16 SC.reconfigure SCsloppy @08:01:30.20 SC.home.seek initial @08:01:33.88 Forearm.seek retract @08:01:33.93 Elbow.seek hammer @08:01:35.08 Forearm.reconfigure TightForearm @08:01:35.13 Forearm.seek 2 @08:01:36.19 Forearm.seek retract @08:01:37.29 Forearm.reconfigure LooseForearm @08:01:37.77PDT19-Mar-14 ===> STARTED wqPCR (phase #15) <=== @08:01:37.78 Starting initialPurge @08:01:37.79 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:01:37.84 Hand.release @08:01:38.66 Elbow.reconfigure TightElbow @08:01:38.69 Elbow.seek FlushPuck::Garage @08:01:40.47 Elbow.reconfigure LooseElbow @08:01:40.55 Forearm.seek garage @08:01:41.52 Hand.grip @08:01:42.29 Forearm.seek retract @08:01:43.29 Elbow.reconfigure TightElbow @08:01:43.32 Elbow.seek CC @08:01:46.13 Elbow.reconfigure LooseElbow @08:01:46.21 Forearm.reconfigure TightForearm @08:01:46.24 Forearm.seek CC @08:01:47.37 CC.seek closed @08:01:51.13 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.95 @08:01:51.18 Hand.release @08:01:51.99 Forearm.reconfigure LooseForearm @08:01:52.02 Forearm.seek retract @08:01:52.99 Flushing the Collection Valve::Manifold @08:01:53.08 CS Pulling 5ml of flush @08:01:53.09 CSV.dial CSR @08:01:54.35 CSR.select :flush @08:01:54.45 CS.reconfigure CSconfig @08:01:54.49 CS.seek 5.000ml @08:02:07.99 delay 5 seconds @08:02:13.00 CSR.select :air @08:02:13.14 CS.reconfigure CSfast @08:02:13.17 CS.seek 7.500ml @08:02:19.89 CSV.dial :waste @08:02:21.15 CS.reconfigure CSconfig @08:02:21.19 CS.seek 0.000ml @08:02:41.02 Purging external Intake @08:02:41.12 CSV.dial :flush @08:02:41.75 CS.reconfigure CSfast @08:02:41.79 CS.seek 10.000ml @08:03:05.74 delay 5 seconds @08:03:10.75 CSV.dial CBV @08:03:10.77
CBV.dial CSV @08:03:11.49 CTV.dial :intake @08:03:12.25 Intake.select :open @08:03:12.34 CS.seek 0.000ml @08:03:36.83 Intake.select :close @08:03:36.92 CTV.dial :air @08:03:37.66 Flushing upper manifold @08:03:37.75 CSV.dial :flush @08:03:38.54 CS.seek 5.000ml @08:03:50.83 delay 5 seconds @08:03:55.84 CSV.dial :air @08:03:56.33 CS.seek 7.000ml @08:04:01.77 CSV.dial CBV @08:04:01.79
CBV.dial CSV @08:04:04.28 CTV.dial :air @08:04:04.30 CS.seek 0.000ml @08:04:20.98 delay 7 seconds @08:04:28.03 CS.seek 3.000ml @08:04:35.80 CSV.dial :waste @08:04:36.23 CS.seek 0.000ml @08:04:44.00 CSV.dial :flush @08:04:44.43 CS.seek 3.000ml @08:04:52.17 delay 5 seconds @08:04:57.18 CSV.dial :air @08:04:57.66 CS.seek 5.000ml @08:05:03.14 CSV.dial :air @08:05:03.16 CS.seek 0.000ml @08:05:15.39 delay 7 seconds @08:05:22.41 CS.seek 3.000ml @08:05:30.14 CSV.dial :waste @08:05:30.93 CS.seek 0.000ml @08:05:38.64 Garaging FlushPuck in Collection Clamp @08:05:38.70 Elbow.reconfigure TightElbow @08:05:38.73 Elbow.seek CC @08:05:38.94 Elbow.reconfigure LooseElbow @08:05:39.03 Forearm.seek CC @08:05:39.97 CC.seek sealed @08:05:40.95 Hand.grip @08:05:41.69 CC.seek open @08:05:44.98 Forearm.seek retract @08:05:46.47 Elbow.reconfigure TightElbow @08:05:46.50 Elbow.seek garage @08:05:49.34 Elbow.reconfigure LooseElbow @08:05:49.42 Forearm.reconfigure TightForearm @08:05:49.45 Forearm.seek garage @08:05:50.60 Hand.release @08:05:51.35 Forearm.reconfigure LooseForearm @08:05:51.38 Forearm.seek retract @08:05:52.51 Starting mfb @08:05:52.52 pcrBAC sampling at most 1000ml, wcr at most 1000ml @08:05:52.54 Loading sh1 puck from tube 3 into Collection Clamp @08:05:52.57 BACfans.email "Can@08:01:37, 14.9C, 71% humidity, 14.2psia, 13.729V, 0.616A, 0.616A avg, 8.46W\nCTD data unavailable\nISUS data unavailable",Subject => "pcrBAC sampling at most 1000ml, wcr at most 1000ml" @08:05:54.27 Readying the MFB @08:05:54.28 Homing MFB Syringe @08:05:54.29 AFV.select :holdingCoil @08:05:54.46
SC.reconfigure SCtight @08:05:54.50 SC.seek 3 @08:05:54.59 ATV.dial :air2 @08:05:55.88 AS.reconfigure ASconfig @08:05:55.92 AS.home.seek home @08:05:56.76
SC.reconfigure SCloose @08:05:56.94 Elbow.reconfigure TightElbow @08:05:56.97 Elbow.seek 3 @08:05:58.87 Forearm.reconfigure TightForearm @08:05:58.90 Forearm.seek 3 + 800 counts @08:05:59.92 SE.reconfigure SEpick @08:05:59.97 SE.seek beamBreak @08:06:02.91 SE.configure SEconfig @08:06:03.04 Elbow.seek 3 @08:06:03.29 Forearm.seek 3 + 300 counts @08:06:03.63 Forearm.reconfigure LooseForearm @08:06:03.67 Hand.grip @08:06:03.87 AFV.select :carrierSolution @08:06:03.97 delay 2 seconds @08:06:04.46 SE.seek down @08:06:04.59
Forearm.seek retract @08:06:05.53 Elbow.seek CC @08:06:05.98 AS.seek 1.000ml @08:06:07.07
Elbow.reconfigure LooseElbow @08:06:07.13 SE.coast @08:06:07.20
Forearm.reconfigure TightForearm @08:06:07.24 Forearm.seek CC @08:06:08.36 CC.seek closed @08:06:12.16 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.96 @08:06:12.17 Hand.release @08:06:12.98 Forearm.reconfigure LooseForearm @08:06:13.01 Forearm.seek retract @08:06:13.94 SC.coast @08:06:13.97 collectSample 1000ml w/bubble point of 55psi Priming 7ml @08:06:13.98 Sleepy.power :sampler,:ON @08:06:13.99 Intake.select :close @08:06:14.00 Exhaust.select :close @08:06:14.01 SS.hold @08:06:14.16 Calibrating pressure and temperature sensors @08:06:14.17 CTV.dial :air @08:06:14.18 SSV.dial :spit,avoiding => exhaust @08:06:14.19 SS.reconfigure SSfast @08:06:14.23 SS.seek 5.00ml @08:06:17.19 SSV.dial CBV @08:06:17.21
CBV.dial SSV @08:06:18.55 delay 3.8 seconds @08:06:22.43 SSV.dial :spit @08:06:23.74 SS.seek 0.00ml @08:06:26.49 SSV.dial CBV @08:06:28.29 CTV.dial :intake @08:06:29.02 Waiting for external sampler to finish priming... @08:06:29.03 delayUntil "08:16:13.98" @08:06:57.70 delay 1 second @08:06:58.71 AFV.select :holdingCoil @08:06:58.80 delay 1 second @08:06:59.81 ATV.dial :waste @08:07:00.72 AS.reconfigure ASnorm @08:07:00.75 AS.home.seek empty @08:07:54.04 ATV.dial :waste @08:07:54.05 ARV.dial between carrier and air1 @08:07:54.12 ASV.dial :waste1 @08:16:13.99 SSV.dial CBV @08:16:14.01
CBV.dial SSV @08:16:14.02 Allowing 55s for 7.0ml of remaining 7.0ml Intake.select :open @08:16:16.15 Intake @14.6psia, vacuum from 8.1 to 12.1psi @08:16:16.16 SS.reconfigure forSampling @08:16:16.20 SS.seek 7.00ml @08:16:21.60 Stroke completed -- waiting for pressure to equalize ... @08:16:24.00 Intake.select :close @08:16:24.13 SSV.dial :exhaust @08:16:24.88 Exhaust.select :open @08:16:24.98 SS.reconfigure SSexhale @08:16:25.02 SS.seek 0.00ml @08:16:28.63 Exhaust.select :close @08:16:28.73 Sampling 1000.0ml @08:16:28.74 Intake.select :close @08:16:28.75 Exhaust.select :close @08:16:28.76 SS.reconfigure SSconfig @08:16:28.79 SS.hold @08:16:28.94 CTV.dial :intake @08:16:28.95 SSV.dial CBV @08:16:28.97
CBV.dial SSV @08:16:29.75 Allowing 185s for 25.0ml of remaining 1000.0ml Intake.select :open @08:16:31.88 Intake @14.2psia, vacuum from 7.7 to 11.7psi @08:16:31.89 SS.reconfigure forSampling @08:16:31.93 SS.seek 25.00ml @08:16:33.54 Vacuum too high -- waiting up to 40s @08:17:13.69 SS.reconfigure SSconfig @08:17:13.72 SS.hold @08:17:13.80 Intake.select :close @08:17:13.96 ShallowSampler::Clogged in MAIN -- Intake @14.1psia, & Outlet @2.1psia -- no progress after 0.7ml SSV.dial :exhaust @08:17:14.73 Exhaust.select :open @08:17:14.84 SS.reconfigure SSexhale @08:17:14.88 SS.seek 0.00ml @08:17:16.00 Exhaust.select :close @08:17:16.10 Sampled 0.7ml @08:17:16.11 collectSample: Complete @08:17:16.12 Evacuating Puck (bubblePt=55.0psi, delay=15.0s) Intake.select :close @08:17:16.18 CSV.dial :waste @08:17:16.21 CS.seek 0.000ml @08:17:16.31 SSV.dial CBV,avoiding => exhaust @08:17:16.33
CBV.dial SSV @08:17:16.37 CSV.dial :air @08:17:17.16 CS.seek 10.000ml @08:17:18.20
CTV.dial :air @08:17:18.95 delay 15 seconds @08:17:19.09 Sleepy.power :sampler,:OFF @08:17:33.95
SSV.dial :spit,avoiding => exhaust @08:17:35.27 CTV.dial CSV,avoiding => intake @08:17:35.29
SS.seek 0.00ml @08:17:35.38 SSV.dial CBV,avoiding => exhaust @08:17:41.04 CBV.dial between CSV and waste2b @08:17:41.98 CTV.dial CSV,avoiding => air @08:17:42.00
CSV.dial CTV @08:17:42.45 CS.reconfigure CSpressurize @08:17:42.48 CS.seek 0.000ml @08:18:08.36 CSV.dial :air @08:18:08.89 CS.reconfigure CSfast @08:18:08.92 CS.seek 10.000ml @08:18:32.43 CSV.dial between air and CTV @08:18:32.82 CS.seek 3.015ml @08:18:49.50 CTV.dial CSV,avoiding => air @08:18:49.51
CSV.dial CTV @08:18:49.81 CS.reconfigure CSpressurize @08:18:49.84 CS.seek 0.000ml @08:18:54.79 BubblePoint: Intake @61.2psia, Outlet @6.1psia @08:18:54.80 SS.reconfigure SSconfig @08:18:54.83 SS.seek 10.00ml @08:19:00.46 delay 15 seconds @08:19:15.56 Evacuated: Intake @60.3psia, Outlet @-0.3psia @08:19:15.57 SSV.dial :spit,avoiding => exhaust @08:19:16.89 SS.reconfigure SSexhale @08:19:16.92 SS.seek 0.00ml @08:19:21.76 SS.reconfigure SSfast @08:19:21.79 SS.seek 5.00ml @08:19:24.47 SSV.dial CBV,avoiding => exhaust @08:19:24.49
CBV.dial SSV @08:19:25.82 CTV.dial :air,avoiding => intake @08:19:27.11 CSV.dial CTV @08:19:27.13
SS.reconfigure SSconfig @08:19:27.17 SS.seek 10.00ml @08:19:28.13 CSV.dial :air @08:19:28.98 CS.reconfigure CSfast @08:19:29.01 CS.seek 0.000ml @08:19:30.40
SSV.dial :spit,avoiding => exhaust @08:19:31.72 SS.reconfigure SSexhale @08:19:31.75 SS.seek 0.00ml @08:19:32.67 CS.coast @08:19:36.82
SS.reconfigure SSfast @08:19:36.86 SS.seek 5.00ml @08:19:39.55 SSV.dial CBV,avoiding => exhaust @08:19:39.57
CBV.dial SSV @08:19:41.88 SSV.dial :spit,avoiding => exhaust @08:19:43.20 SS.reconfigure SSconfig @08:19:43.23 SS.seek 0.00ml @08:19:46.37 SS.coast @08:19:46.40 Evacuating Puck (bubblePt=55.0psi, delay=15.0s) Intake.select :close @08:19:46.46 CSV.dial :waste @08:19:46.55 SSV.dial CBV,avoiding => exhaust @08:19:46.57
CBV.dial SSV @08:19:47.31 CS.seek 0.000ml @08:19:47.36 CSV.dial :air @08:19:47.89
CTV.dial :air @08:19:47.90 delay 15 seconds @08:19:48.15 CS.seek 10.000ml @08:20:02.90
SSV.dial :spit,avoiding => exhaust @08:20:04.73 CTV.dial CSV,avoiding => intake @08:20:04.75
SS.reconfigure SSexhale @08:20:04.81 SS.seek 0.00ml @08:20:04.87 SSV.dial CBV,avoiding => exhaust @08:20:11.94 CBV.dial between CSV and waste2b @08:20:12.86 CSV.dial between air and CTV @08:20:13.16 CS.seek 8.190ml @08:20:18.18 CTV.dial CSV,avoiding => air @08:20:18.20
CSV.dial CTV @08:20:18.49 CS.reconfigure CSpressurize @08:20:18.52 CS.seek 0.000ml @08:20:39.76 CSV.dial :air @08:20:40.29 CS.reconfigure CSfast @08:20:40.32 CS.seek 10.000ml @08:21:03.90 CSV.dial between air and CTV @08:21:04.29 CS.seek 3.012ml @08:21:20.90 CTV.dial CSV,avoiding => air @08:21:20.91
CSV.dial CTV @08:21:21.21 CS.reconfigure CSpressurize @08:21:21.24 CS.seek 0.000ml @08:21:28.17 BubblePoint: Intake @69.5psia, Outlet @14.4psia @08:21:28.18 SS.reconfigure SSconfig @08:21:28.22 SS.seek 10.00ml @08:21:33.75 delay 15 seconds @08:21:48.85 Evacuated: Intake @68.1psia, Outlet @0.1psia SSV.dial :spit,avoiding => exhaust @08:21:50.18 SS.reconfigure SSexhale @08:21:50.21 SS.seek 0.00ml @08:21:55.31 SS.reconfigure SSfast @08:21:55.34 SS.seek 5.00ml @08:21:57.79 SSV.dial CBV,avoiding => exhaust @08:21:57.81
CBV.dial SSV @08:21:59.14 CTV.dial :air,avoiding => intake @08:22:00.43 CSV.dial CTV @08:22:00.45
SS.reconfigure SSconfig @08:22:00.49 SS.seek 10.00ml @08:22:01.45 CSV.dial :air @08:22:01.96 CS.reconfigure CSfast @08:22:01.99 CS.seek 0.000ml @08:22:03.69
SSV.dial :spit,avoiding => exhaust @08:22:03.92 CS.coast @08:22:05.01
SS.reconfigure SSexhale @08:22:05.04 SS.seek 0.00ml @08:22:10.31 SS.reconfigure SSfast @08:22:10.34 SS.seek 5.00ml @08:22:12.84 SSV.dial CBV,avoiding => exhaust @08:22:12.86
CBV.dial SSV @08:22:15.16 SSV.dial :spit,avoiding => exhaust @08:22:16.46 SS.reconfigure SSconfig @08:22:16.49 SS.seek 0.00ml @08:22:19.62 SS.coast @08:22:19.65 Lysing: Adding 1.5ml lysis CBV.dial between CSV and waste1,avoiding => DBV @08:22:21.13 CTV.dial CSV @08:22:22.38 CSV.dial CSR @08:22:23.16 CSR.select :lysis @08:22:23.26 CS.reconfigure CSconfig @08:22:23.30 CS.seek 1.000ml @08:22:26.57 delay 5 seconds @08:22:31.59 CSR.select :air @08:22:31.73 CS.seek 5.999ml @08:22:45.13 delay 2 seconds @08:22:47.14 CSV.dial :waste1 @08:22:48.39 CS.reconfigure CSfast @08:22:48.42 CS.seek empty @08:23:02.76 CSV.dial CSR @08:23:04.02 CSR.select :lysis @08:23:04.12 CS.reconfigure CSconfig @08:23:04.16 CS.seek 1.500ml @08:23:08.88 delay 5 seconds @08:23:13.89 CSR.select :air @08:23:14.03 CS.seek 4.500ml @08:23:22.41 delay 2 seconds @08:23:24.42 CSV.dial CTV @08:23:24.92 CS.seek 1.275ml @08:23:33.89 Heating sh1 puck from tube 3 in Collection Clamp to 85.0C for 5:00 @08:23:33.90 CH.seek 85.0C,8:00 @08:26:30.26 CH.hold 5:00,85.0C @08:31:30.38 Collection Puck Heater at 84.9C after lysing @08:31:30.39 CS.seek 0.000ml @08:31:34.47 CTV.dial between spare and air,avoiding => intake @08:31:35.46 CSV.dial :air @08:31:35.89 CS.seek 5.000ml @08:31:49.46 CSV.dial CTV @08:31:49.96 delay 2 seconds @08:31:51.97 CSV.dial :air @08:31:52.46 delay 2 seconds @08:31:54.47 CSV.dial CTV @08:31:54.96 delay 2 seconds @08:31:56.97 CSV.dial :air @08:31:57.46 delay 2 seconds @08:31:59.47 CS.seek 0.000ml @08:32:12.99 Recovering Lysate into Collection syringe @08:32:13.00 CSV.dial CBV @08:32:13.99 CBV.dial CSV @08:32:14.34 CS.seek 5.000ml @08:32:27.80 delay 5 seconds @08:32:32.81 CTV.dial :air @08:32:33.28 CS.seek 6.999ml @08:32:39.80 delay 5 seconds @08:32:44.81 CSV.dial :air2 @08:32:45.24 CS.seek 10.000ml @08:32:53.65 CSV.dial between air and CTV,avoiding => CBV @08:32:54.78 Lysing Complete @08:32:54.83 Storing sh1 puck in Collection Clamp to tube 2 @08:32:54.93 SE.seek down @08:32:55.25 SC.reconfigure SCtight @08:32:55.28 SC.seek 2 @08:32:57.46 SC.reconfigure SCloose @08:32:57.64 Elbow.reconfigure TightElbow @08:32:57.67 Elbow.seek 2 @08:32:59.34 Forearm.reconfigure TightForearm @08:32:59.37 Forearm.seek 2 + 800 counts @08:33:00.46 SE.reconfigure SEpick @08:33:00.51 SE.seek beamBreak @08:33:01.66 SE.configure SEconfig @08:33:01.78 Elbow.reconfigure LimpElbow @08:33:01.81 Forearm.reconfigure LooseForearm @08:33:01.85 Forearm.seek 2 @08:33:02.14 Elbow.hold @08:33:02.19 Elbow.reconfigure TightElbow @08:33:02.27 SE.seek 19.02 Pucks below Top Plate @08:33:02.62 Forearm.coast @08:33:02.65 Forearm.seek 2 + 298 counts @08:33:02.88 Forearm.reconfigure TightForearm @08:33:02.91 Forearm.seek 2 + 800 counts @08:33:03.14 SE.seek 20.02 Pucks below Top Plate @08:33:03.70 Forearm.reconfigure LooseForearm @08:33:03.73 Forearm.seek retract @08:33:04.65 Elbow.seek CC @08:33:06.20 Elbow.reconfigure LooseElbow @08:33:06.29 Forearm.seek CC @08:33:07.23 CC.seek sealed @08:33:08.18 Hand.grip @08:33:08.89 CC.seek open @08:33:12.21 Forearm.seek retract @08:33:13.16 Elbow.reconfigure TightElbow @08:33:13.20 Elbow.seek 2 @08:33:14.84 Elbow.reconfigure LimpElbow @08:33:14.89 Forearm.seek 2 @08:33:15.92 Elbow.hold @08:33:15.97 Elbow.reconfigure TightElbow @08:33:16.01 Hand.release @08:33:16.84 Forearm.coast @08:33:16.87 Forearm.seek 2 + 321 counts @08:33:17.10 Forearm.reconfigure TightForearm @08:33:17.13 Forearm.seek 2 + 800 counts @08:33:17.41 SE.seek down @08:33:18.13 SE.coast @08:33:18.52
Forearm.reconfigure LooseForearm @08:33:18.55 Forearm.seek retract @08:33:19.46 SC.coast @08:33:19.49 Tranferring lysate to PRV @08:33:19.50 CSV.dial :air @08:33:19.80 CS.seek 10.000ml @08:33:19.86 CSV.dial between air and CTV @08:33:20.25 AIV.dial :bypass @08:33:20.31 Beginning samplePassC @08:33:20.32 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:33:20.47 Elbow.seek FlushPuck::Garage @08:33:22.24 Elbow.reconfigure LooseElbow @08:33:22.32 Forearm.seek garage @08:33:23.33 Hand.grip @08:33:24.09 Forearm.seek retract @08:33:25.13 Elbow.reconfigure TightElbow @08:33:25.17 Elbow.seek CC @08:33:27.95 Elbow.reconfigure LooseElbow @08:33:28.04 Forearm.reconfigure TightForearm @08:33:28.68 Forearm.seek CC @08:33:29.81 CC.seek closed @08:33:33.57 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.98 @08:33:33.62 Hand.release @08:33:34.43 Forearm.reconfigure LooseForearm @08:33:34.46 Forearm.seek retract @08:33:35.41 AWV.select :air @08:33:35.42 AIV.dial :bypass @08:33:35.43 PSV.dial PSR @08:33:36.72 PSR.select :flush @08:33:36.82 PS.seek 1.500ml @08:33:52.90 delay 5 seconds @08:33:57.91 PSV.dial :waste1 @08:33:58.34 PS.seek 0.000ml @08:34:14.40 PSV.dial PSR @08:34:14.84 PSR.select :air @08:34:14.94 PS.seek 2.500ml @08:34:41.19 CBV.dial PRV @08:34:41.20 PRV.dial CBV @08:34:41.96
PSV.dial :mixloop @08:34:43.07 CTV.dial :air,avoiding => intake @08:34:43.08 PTV.dial :PRVmixing @08:34:43.16 PS.seek 0.000ml @08:35:09.37 PSV.dial :air @08:35:09.83 PS.seek 2.500ml @08:35:36.06 PSV.dial :mixloop @08:35:36.52 PS.seek 0.000ml @08:36:02.81 PSV.dial :air @08:36:03.27 PS.seek 2.500ml @08:36:29.59 PSV.dial :mixloop @08:36:30.05 PS.seek 0.000ml @08:36:56.49 Garaging FlushPuck in Collection Clamp @08:36:56.55 Elbow.reconfigure TightElbow @08:36:56.58 Elbow.seek CC @08:36:56.68 Elbow.reconfigure LooseElbow @08:36:56.76 Forearm.seek CC @08:36:57.74 CC.seek sealed @08:36:58.67 Hand.grip @08:36:59.38 CC.seek open @08:37:02.66 Forearm.seek retract @08:37:03.73 Elbow.reconfigure TightElbow @08:37:03.77 Elbow.seek garage @08:37:06.58 Elbow.reconfigure LooseElbow @08:37:06.67 Forearm.reconfigure TightForearm @08:37:06.70 Forearm.seek garage @08:37:07.82 Hand.release @08:37:08.54 Forearm.reconfigure LooseForearm @08:37:08.57 Forearm.seek retract @08:37:09.57 Connecting PS to CS to transfer sample @08:37:09.58 CBV.dial AIV @08:37:09.59 AIV.dial :bypass @08:37:09.60 PSV.dial :mixloop @08:37:09.61 PTV.dial :PRVmixing PRV.dial AIV @08:37:09.62 CTV.dial :air,avoiding => intake @08:37:09.63 Loading lyfil puck from tube 3 into Collection Clamp @08:37:09.78 SE.seek down @08:37:10.10 SC.reconfigure SCtight @08:37:10.13 SC.seek 3 @08:37:12.30 SC.reconfigure SCloose @08:37:12.48 Elbow.reconfigure TightElbow @08:37:12.51 Elbow.seek 3 @08:37:14.38 Forearm.reconfigure TightForearm @08:37:14.41 Forearm.seek 3 + 800 counts @08:37:15.51 SE.reconfigure SEpick @08:37:15.56 SE.seek beamBreak @08:37:18.52 SE.configure SEconfig @08:37:18.64 Elbow.seek 3 @08:37:18.79 Forearm.seek 3 + 300 counts @08:37:19.02 Forearm.reconfigure LooseForearm @08:37:19.06 Hand.grip @08:37:19.75 SE.seek down @08:37:19.90
Forearm.seek retract @08:37:21.50 Elbow.seek CC @08:37:22.51 SE.coast @08:37:23.07
Elbow.reconfigure LooseElbow @08:37:23.15 Forearm.reconfigure TightForearm @08:37:23.18 Forearm.seek CC @08:37:24.28 CC.seek closed @08:37:28.07 Collection Clamp onSpring/offSpring for 5/22 samples, on/off torque 2.02 @08:37:28.08 Hand.release @08:37:28.79 Forearm.reconfigure LooseForearm @08:37:28.82 Forearm.seek retract @08:37:29.76 SC.coast @08:37:29.78 CTV.dial CSV,avoiding => intake @08:37:31.07 CSV.dial CTV @08:37:31.36 CS.seek 5.000ml @08:37:44.76 delay 1:20 @08:39:04.77 CS.seek 2.500ml @08:39:11.88 delay 40 seconds @08:39:51.89 CS.seek 0.000ml @08:39:59.07 delay 1:00 @08:40:59.08 PSV.dial :air @08:40:59.52 PS.seek 2.500ml @08:41:25.83 PSV.dial :mixloop @08:41:26.26 delay 3 seconds @08:41:29.27 PSV.dial :air @08:41:29.70 delay 2 seconds @08:41:31.71 PSV.dial :mixloop @08:41:32.15 delay 3 seconds @08:41:35.16 PSV.dial :waste1 @08:41:36.44 PS.seek empty @08:42:02.67 CBV.dial :waste1 @08:42:03.12 delay 5 seconds @08:42:08.13 CBV.dial AIV @08:42:08.58 delay 10 seconds @08:42:18.59 CBV.dial :waste1 @08:42:19.06 delay 10 seconds @08:42:29.07 CBV.dial AIV @08:42:29.53 delay 10 seconds @08:42:39.54 CTV.dial CSV,avoiding => intake @08:42:39.55 CBV.dial between AIV and waste1 @08:42:39.87 CSV.dial :air @08:42:40.38 CS.seek 10.000ml @08:43:06.43 CSV.dial CTV @08:43:06.94 CS.seek 0.000ml @08:43:33.03 CBV.dial :waste1 @08:43:33.32 delay 10 seconds @08:43:43.33 CTV.dial :air,avoiding => intake @08:43:44.68 Storing lyfil puck in Collection Clamp to tube 2 @08:43:44.77 SE.seek down @08:43:45.08 SC.reconfigure SCtight @08:43:45.11 SC.seek 2 @08:43:47.34 SC.reconfigure SCloose @08:43:47.52 Elbow.reconfigure TightElbow @08:43:47.55 Elbow.seek 2 @08:43:49.13 Forearm.reconfigure TightForearm @08:43:49.16 Forearm.seek 2 + 800 counts @08:43:50.18 SE.reconfigure SEpick @08:43:50.23 SE.seek beamBreak @08:43:51.50 SE.configure SEconfig @08:43:51.62 Elbow.reconfigure LimpElbow @08:43:51.65 Forearm.reconfigure LooseForearm @08:43:51.69 Forearm.seek 2 @08:43:51.92 Elbow.hold @08:43:51.97 Elbow.reconfigure TightElbow @08:43:52.05 SE.seek 20.02 Pucks below Top Plate @08:43:52.41 Forearm.coast @08:43:52.44 Forearm.seek 2 + 335 counts @08:43:52.68 Forearm.reconfigure TightForearm @08:43:52.71 Forearm.seek 2 + 800 counts @08:43:52.90 SE.seek 21.02 Pucks below Top Plate @08:43:53.58 Forearm.reconfigure LooseForearm @08:43:53.61 Forearm.seek retract @08:43:54.53 Elbow.seek CC @08:43:56.08 Elbow.reconfigure LooseElbow @08:43:56.16 Forearm.seek CC @08:43:57.12 CC.seek sealed @08:43:58.07 Hand.grip @08:43:58.75 CC.seek open @08:44:02.13 Forearm.seek retract @08:44:03.08 Elbow.reconfigure TightElbow @08:44:03.12 Elbow.seek 2 @08:44:05.28 Elbow.reconfigure LimpElbow @08:44:05.33 Forearm.seek 2 @08:44:06.33 Elbow.hold @08:44:06.38 Elbow.reconfigure TightElbow @08:44:06.42 Hand.release @08:44:07.20 Forearm.coast @08:44:07.23 Forearm.seek 2 + 314 counts @08:44:07.48 Forearm.reconfigure TightForearm @08:44:07.51 Forearm.seek 2 + 800 counts @08:44:07.80 SE.seek down @08:44:08.43 SE.coast @08:44:08.90
Forearm.reconfigure LooseForearm @08:44:08.94 Forearm.seek retract @08:44:09.88 SC.coast @08:44:09.91 PSV.dial :air @08:44:10.96 PS.seek 0.499ml @08:44:16.92 delay 3 seconds @08:44:19.94 PSV.dial :mixloop @08:44:20.38 PRV.dial :air @08:44:20.81 delay 3 seconds @08:44:23.82 PRV.dial CBV @08:44:24.41 CBV.dial AIV @08:44:24.86 PRV.dial between CBV and air @08:44:25.17 PRV.dial :air @08:44:25.52 PS.reconfigure PSslow @08:44:25.55 PS.seek 0.599ml @08:44:28.24 PRV.dial :waste2 @08:44:28.98 PS.reconfigure PSconfig @08:44:29.01 PS.seek empty @08:44:35.95 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:44:36.10 Elbow.seek FlushPuck::Garage @08:44:37.84 Elbow.reconfigure LooseElbow @08:44:37.93 Forearm.seek garage @08:44:38.95 Hand.grip @08:44:39.83 Forearm.seek retract @08:44:40.84 Elbow.reconfigure TightElbow @08:44:40.88 Elbow.seek CC @08:44:43.69 Elbow.reconfigure LooseElbow @08:44:43.77 Forearm.reconfigure TightForearm @08:44:43.80 Forearm.seek CC @08:44:44.92 CC.seek closed @08:44:48.68 Collection Clamp onSpring/offSpring for 5/22 samples, on/off torque 1.99 @08:44:48.74 Hand.release @08:44:49.53 Forearm.reconfigure LooseForearm @08:44:49.56 Forearm.seek retract @08:44:50.56 Clearing PML for modified lysate CTV.dial :air,avoiding => intake @08:44:50.57 PRV.dial :waste2 @08:44:50.58 PTV.dial :PRV,avoiding => puck @08:44:51.31 delay 2 seconds @08:44:53.31 PTV.dial :PRVmixing,avoiding => puck @08:44:54.03 delay 2 seconds @08:44:56.04 PSV.dial :PML @08:44:56.05 delay 2 seconds @08:44:58.05 PRV.dial :air @08:44:58.78 delay 2 seconds @08:45:00.84 PS.seek 1.499ml @08:45:16.98 delay 5 seconds @08:45:21.99 PS.seek 2.500ml @08:45:33.01 PSV.dial :air @08:45:33.48 PS.seek 0.000ml @08:45:59.76 mixing lysate with speDil @08:45:59.77 PSV.dial :PML @08:46:00.20 CBV.dial :PRV @08:46:00.21 PRV.dial :air @08:46:00.22 delay 2 seconds @08:46:02.23 PRV.dial :speDil @08:46:02.67 delay 2 seconds @08:46:04.73 PS.reconfigure PSslow1 @08:46:04.76 PS.seek 0.225ml @08:46:28.11 delay 5 seconds @08:46:33.12 PRV.dial CBV @08:46:33.86 delay 2 seconds @08:46:35.92 PS.seek 0.475ml @08:47:01.89 delay 5 seconds @08:47:06.90 PRV.dial :air @08:47:07.35 delay 2 seconds @08:47:09.91 PS.seek 0.925ml @08:47:56.04 delay 10 seconds @08:48:06.05 PRV.dial CBV @08:48:06.51 CTV.dial :air,avoiding => intake @08:48:06.52 PSV.dial :air @08:48:06.97 PS.reconfigure PSconfig @08:48:07.00 PS.seek 2.500ml @08:48:24.04 delay 3 seconds @08:48:27.05 PSV.dial PTV @08:48:27.77 PTV.dial :PRV,avoiding => puck @08:48:28.50 PS.seek 0.000ml @08:48:54.69 PSV.dial :air @08:48:55.42 PS.seek 2.500ml @08:49:21.60 delay 3 seconds @08:49:24.61 PSV.dial PTV @08:49:25.33 PTV.dial :PRV,avoiding => puck @08:49:25.35 PS.seek 0.000ml @08:49:51.63 Pushing modified lysate into AIV Injection Loop @08:49:51.64 CBV.dial AIV @08:49:51.65 AIV.dial :bypass @08:49:51.66 PRV.dial CBV @08:49:51.67 PSV.dial :air @08:49:52.39 PS.seek 2.500ml @08:50:18.66 delay 3 seconds @08:50:21.67 PSV.dial :PML @08:50:22.11 PTV.dial :PRVmixing,avoiding => puck @08:50:22.90 PS.reconfigure PSslow1 @08:50:22.93 PS.seek 1.850ml @08:51:29.38 delay 5 seconds @08:51:34.39 AIV.dial :sample @08:51:34.92 AWV.select :waste @08:51:35.08 PS.reconfigure PSslow2 @08:51:35.11 PS.seek 1.310ml @08:53:25.06 delay 15 seconds @08:53:40.07 AIV.dial :bypass @08:53:40.63 PS.reconfigure PSconfig @08:53:40.66 PS.seek 0.000ml @08:53:54.87 PSV.dial :air @08:53:55.32 PS.seek 2.500ml @08:54:21.59 PSV.dial :PML @08:54:22.04 PS.seek 0.000ml @08:54:48.31 AIV.dial :coilToMFB @08:54:48.32 AWV.select :air @08:54:48.46 Extracting Nucleic Acid from AIV @08:54:48.48
Beginning samplePassD @08:54:48.49 SPE.hold 2:46:40,55.0C @08:54:48.51 ASV.dial :SPEdelivery @08:54:48.64
AWV.select :air @08:54:48.65 AIV.dial :bypass @08:54:48.66 PSV.dial PSR @08:54:49.06 delay 20 seconds @08:54:49.79
PSR.select :killMFB @08:54:49.89 PS.seek 1.500ml @08:55:05.90 delay 5 seconds @08:55:09.07 primeLysateINT @08:55:09.08 AWV.select :air @08:55:09.09 AIV.dial :coilToMFB @08:55:09.10 ATV.dial :AIV @08:55:10.44 AS.reconfigure ASslow1 @08:55:10.47 AS.seek 0.239ml @08:55:10.91
PSV.dial :waste1 @08:55:11.38 PS.seek 0.000ml @08:55:27.46 PSV.dial PSR @08:55:27.91 PSR.select :air @08:55:28.01 PS.seek 2.500ml @08:55:54.27 PRV.dial CBV @08:55:54.29
CBV.dial PRV @08:55:54.30 PSV.dial :mixloop @08:55:55.44 CTV.dial :air,avoiding => intake @08:55:55.45 PTV.dial :PRVmixing @08:55:55.47 PS.seek 0.000ml @08:55:59.71 delay 10 seconds @08:56:09.72 ATV.dial :air2 @08:56:10.30 AS.seek 0.269ml @08:56:16.68 ATV.dial :waste2 @08:56:17.04 AS.reconfigure ASnorm @08:56:17.07 AS.seek empty @08:56:21.71
PSV.dial PSR @08:56:22.72 PSR.select :flush @08:56:22.82 PS.seek 1.500ml @08:56:31.24 Lysate primed! @08:56:31.25 AFV.select :holdingCoil @08:56:31.26 ATV.dial :air2 @08:56:31.65 AS.reconfigure ASslow1 @08:56:31.68 AS.seek 0.069ml @08:56:38.90
delay 5 seconds @08:56:43.91 PSV.dial :waste1 @08:56:44.36 PS.seek 0.000ml @08:56:46.28 delay 3 seconds @08:56:49.29 ATV.dial :AIV @08:56:49.87 AS.seek 0.469ml @08:57:00.43
PSV.dial PSR @08:57:00.88 PSR.select :air @08:57:00.98 PS.seek 2.500ml @08:57:27.30 PRV.dial CBV @08:57:27.32
CBV.dial PRV @08:57:27.33 PSV.dial :mixloop @08:57:28.35 CTV.dial :air,avoiding => intake @08:57:28.37 PTV.dial :PRVmixing @08:57:28.38 PS.seek 0.000ml @08:57:54.64 PSV.dial PSR @08:57:55.66 PSR.select :flush @08:57:55.76 PS.seek 1.500ml @08:58:11.71 delay 5 seconds @08:58:11.83
delay 5 seconds @08:58:16.76 Solid Phase Extraction Heater at 52.1C @08:58:16.77 ATV.dial ASV @08:58:17.10 ASV.dial :SPEdelivery @08:58:17.16 AS.seek 0.041ml @08:58:17.36
PSV.dial :waste1 @08:58:17.81 PS.seek 0.000ml @08:58:33.86 PSV.dial PSR @08:58:34.31 PSR.select :air @08:58:34.41 PS.seek 2.500ml @08:59:00.73 PRV.dial CBV @08:59:00.75
CBV.dial PRV @08:59:00.76 PSV.dial :mixloop @08:59:01.77 CTV.dial :air,avoiding => intake @08:59:01.78 PTV.dial :PRVmixing @08:59:01.80 PS.seek 0.000ml @08:59:28.01 PSV.dial PSR @08:59:29.03 PSR.select :flush @08:59:29.13 PS.seek 1.500ml @08:59:44.71 delay 10 seconds @08:59:45.17
delay 5 seconds @08:59:50.18 PSV.dial :waste1 @08:59:50.62 PS.seek 0.000ml @08:59:54.72 rinseLysateLineINT @08:59:54.73 AFV.select :carrierSolution @08:59:54.82 delay 2 seconds @08:59:56.83 AS.reconfigure ASconfig @08:59:56.86 AS.seek 1.000ml @09:00:06.67
PSV.dial PSR @09:00:07.11 PSR.select :air @09:00:07.21 PS.seek 2.500ml @09:00:33.48 PRV.dial CBV @09:00:33.50
CBV.dial PRV @09:00:33.51 PSV.dial :mixloop @09:00:34.61 CTV.dial :air,avoiding => intake @09:00:34.62 PTV.dial :PRVmixing @09:00:34.64 PS.reconfigure PSslow @09:00:34.67 PS.seek 1.200ml @09:00:53.02 delay 1 second @09:00:54.04 AFV.select :holdingCoil @09:00:54.13 delay 1 second @09:00:55.14 ATV.dial :AIV @09:00:55.48 AIV.dial :coilToMFB @09:00:55.49 AWV.select :waste @09:00:55.59 AS.reconfigure ASslow0 @09:00:55.63 AS.seek 0.000ml @09:01:01.55
PSV.dial :waste1 @09:01:02.86 PS.reconfigure PSconfig @09:01:02.89 PS.seek 0.000ml @09:01:15.93 PSV.dial :air @09:01:16.95 PS.seek 2.500ml @09:01:43.14 PSV.dial :mixloop @09:01:43.69 PS.reconfigure PSslow @09:01:43.72 PS.seek 0.000ml @09:02:35.09 PSV.dial :air @09:02:35.54 PS.reconfigure PSconfig @09:02:35.57 PS.seek 2.500ml @09:02:37.96 ATV.dial :air2 @09:02:38.54 AS.reconfigure ASslow1 @09:02:38.57 AS.seek 0.050ml @09:02:49.06 ATV.dial :AIV @09:02:49.65 AS.seek 0.010ml @09:02:58.07 ATV.dial :waste @09:02:59.36 AS.seek empty @09:03:01.80
PSV.dial :mixloop @09:03:01.86 ethanol wash @09:03:01.87 ATV.dial :air2 @09:03:02.26
PS.reconfigure PSslow @09:03:02.29 PS.seek 0.000ml @09:03:02.81 AS.reconfigure ASslow2 @09:03:02.85 AS.seek 0.009ml @09:03:13.99 delay 3 seconds @09:03:17.00 Pulling 0.4ml of Ethanol Wash @09:03:17.01 ATV.dial :ethanol @09:03:17.59 AS.reconfigure ASslow1 @09:03:17.63 AS.seek 0.409ml @09:03:53.64
Cleaning Collection Syringe @09:03:53.65 CSV.dial CSR @09:03:54.07 CSR.select :flush @09:03:54.18 CS.seek 4.000ml @09:04:05.09 delay 5 seconds @09:04:10.10 CSR.select :air @09:04:10.19 CS.seek 10.000ml @09:04:26.18 CSV.dial CTV @09:04:26.20
CTV.dial CSV @09:04:27.52 CBV.dial :waste1 @09:04:28.00 CS.seek 0.000ml @09:04:39.47 delay 3 seconds @09:04:42.48 ATV.dial :air1 @09:04:42.80 delay 3 seconds @09:04:45.86 AS.seek 0.434ml @09:04:51.22 delay 3 seconds @09:04:54.11
CSV.dial CSR @09:04:54.23 ATV.dial :ethanol @09:04:54.62 AS.seek 0.419ml @09:04:54.67
CSR.select :flush @09:04:54.76 CS.seek 4.000ml @09:04:57.93 delay 3 seconds @09:05:00.94 ATV.dial ASV @09:05:02.09 AS.seek 0.092ml @09:05:05.77
delay 5 seconds @09:05:10.78 CSR.select :air @09:05:10.87 CS.seek 10.000ml @09:05:26.93 CSV.dial CTV @09:05:26.95
CTV.dial CSV @09:05:27.48 CBV.dial :waste1 @09:05:27.49 CS.seek 0.000ml @09:05:42.92 Session Begun @09:05:46.15 -> showlog @09:05:54.14
Garaging FlushPuck in Collection Clamp @09:05:54.21 Elbow.reconfigure TightElbow @09:05:54.26 Elbow.seek CC @09:05:54.38 Elbow.reconfigure LooseElbow @09:05:54.47 Forearm.seek CC @09:05:55.46 CC.seek sealed @09:05:56.42 Hand.grip @09:05:57.20 CC.seek open @09:06:00.55 Forearm.seek retract @09:06:01.53 Elbow.reconfigure TightElbow @09:06:01.57 Elbow.seek garage @09:06:04.39 Elbow.reconfigure LooseElbow @09:06:04.48 Forearm.reconfigure TightForearm @09:06:04.53 Forearm.seek garage @09:06:05.69 Hand.release @09:06:06.49 Forearm.reconfigure LooseForearm @09:06:06.54 Forearm.seek retract @09:06:07.55 Whole Cell Archive sampling 1000ml @09:06:07.64 Loading wcr puck from tube 4 into Collection Clamp @09:06:07.81 SE.seek down @09:06:08.14 SC.reconfigure SCtight @09:06:08.20 SC.seek 4 @09:06:09.03 delay 3 seconds @09:06:11.31 -> progress @09:06:12.03 ATV.dial :waste2 @09:06:12.15 SC.reconfigure SCloose @09:06:12.35 Elbow.reconfigure TightElbow @09:06:12.40 Elbow.seek 4 @09:06:12.59 AS.reconfigure ASconfig @09:06:12.63 AS.seek empty @09:06:14.26 Forearm.reconfigure TightForearm @09:06:14.32 Forearm.seek 4 + 800 counts @09:06:15.34 SE.reconfigure SEpick @09:06:15.41 SE.seek beamBreak @09:06:16.41 SE.configure SEconfig @09:06:16.56 Elbow.seek 4 @09:06:16.74 Forearm.seek 4 + 300 counts @09:06:17.00 Forearm.reconfigure LooseForearm @09:06:17.06 Hand.grip @09:06:17.82 SE.seek down @09:06:17.99 Forearm.seek retract @09:06:18.36 ATV.dial :air2 @09:06:18.52 SE.coast @09:06:18.72 AS.reconfigure ASslow1 @09:06:18.76 AS.seek 1.000ml @09:06:18.95 Elbow.seek CC @09:06:20.49 Elbow.reconfigure LooseElbow @09:06:20.59 Forearm.reconfigure TightForearm @09:06:20.64 Forearm.seek CC @09:06:21.79 CC.seek closed @09:06:25.57 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.96 @09:06:25.58 Hand.release @09:06:26.36 Forearm.reconfigure LooseForearm @09:06:26.41 Forearm.seek retract @09:06:27.34 SC.coast @09:06:27.39 collectSample 1000ml w/bubble point of 55psi @09:06:27.40 Priming 7ml @09:06:27.41 Sleepy.power :sampler,:ON @09:06:27.43 Intake.select :close @09:06:27.44 Exhaust.select :close @09:06:27.46 SS.hold @09:06:27.67 Calibrating pressure sensors @09:06:27.68 CTV.dial :air @09:06:28.98 SSV.dial :spit,avoiding => exhaust @09:06:28.99 SS.reconfigure SSfast @09:06:29.06 SS.seek 5.00ml @09:06:32.25 SSV.dial CBV @09:06:32.27 CBV.dial SSV @09:06:33.65 delay 3.8 seconds @09:06:37.54 SSV.dial :spit @09:06:38.87 SS.seek 0.00ml @09:06:41.52 SSV.dial CBV @09:06:42.86 CTV.dial :intake @09:06:43.59 Waiting for external sampler to finish priming... @09:06:43.60 delayUntil "09:16:27.41" @09:09:43.17 delay 5 seconds @09:09:48.19 ATV.dial ASV @09:09:48.92 ASV.dial :SPEdelivery @09:09:48.94 AS.seek 0.020ml @09:13:09.11 delay 5 seconds @09:13:14.13 ATV.dial :air2 @09:13:14.90 AS.seek 0.520ml @09:14:57.17 delay 5 seconds @09:15:02.18 ATV.dial ASV @09:15:02.91 ASV.dial :wasteR @09:15:03.53 AS.seek 0.300ml @09:15:48.69 delay 20 seconds @09:16:08.70 AS.seek 0.070ml @09:16:27.41 SSV.dial CBV @09:16:27.43 CBV.dial SSV @09:16:27.44 Allowing 55s for 7.0ml of remaining 7.0ml @09:16:27.46 Intake.select :open @09:16:29.62 Intake @15.0psia, vacuum from 8.5 to 12.5psi @09:16:29.63 SS.reconfigure forSampling @09:16:29.69 SS.seek 7.00ml @09:16:34.80 Stroke completed -- waiting for pressure to equalize ... @09:16:35.44 Intake.select :close @09:16:35.58 SSV.dial :exhaust @09:16:36.35 Exhaust.select :open @09:16:36.46 SS.reconfigure SSexhale @09:16:36.51 SS.seek 0.00ml @09:16:40.55 Exhaust.select :close @09:16:40.66 Sampling 1000.0ml @09:16:40.68 Intake.select :close @09:16:40.69 Exhaust.select :close @09:16:40.71 SS.reconfigure SSconfig @09:16:40.76 SS.hold @09:16:40.93 CTV.dial :intake @09:16:40.94 SSV.dial CBV @09:16:40.97 CBV.dial SSV @09:16:41.75 Allowing 185s for 25.0ml of remaining 1000.0ml @09:16:41.76 Intake.select :open @09:16:43.90 Intake @14.8psia, vacuum from 8.3 to 12.3psi @09:16:43.92 SS.reconfigure forSampling @09:16:43.98 SS.seek 25.00ml @09:16:55.84 delay 20 seconds @09:16:57.85 Stroke completed -- waiting for pressure to equalize ... @09:16:58.50 Intake.select :close @09:16:58.65 SSV.dial :exhaust @09:16:59.43 Exhaust.select :open @09:16:59.54 SS.reconfigure SSexhale @09:16:59.59 SS.seek 0.00ml @09:17:08.33 Exhaust.select :close @09:17:08.43 SSV.dial CBV @09:17:08.45 CBV.dial SSV @09:17:09.24 Allowing 185s for 25.0ml of remaining 975.0ml @09:17:09.26 Intake.select :open @09:17:11.41 Intake @15.2psia, vacuum from 8.7 to 12.7psi @09:17:11.43 SS.reconfigure forSampling @09:17:11.49 SS.seek 25.00ml @09:17:15.85 ATV.dial :waste2 @09:17:16.49 AS.reconfigure ASconfig @09:17:16.53 AS.seek empty @09:17:20.97 Elution @09:17:20.98 ATV.dial :air1 @09:17:21.97 AS.reconfigure ASslow1 @09:17:22.01 AS.seek 0.049ml @09:17:25.38 Stroke completed -- waiting for pressure to equalize ... @09:17:26.02 Intake.select :close @09:17:26.16 SSV.dial :exhaust @09:17:26.94 Exhaust.select :open @09:17:27.06 SS.reconfigure SSexhale @09:17:27.11 SS.seek 0.00ml @09:17:32.54 delay 3 seconds @09:17:35.55 ATV.dial :ElutionWater @09:17:36.14 Exhaust.select :close @09:17:36.17 AS.seek 0.109ml @09:17:36.30 SSV.dial CBV @09:17:36.33 CBV.dial SSV @09:17:37.11 Allowing 185s for 25.0ml of remaining 950.0ml @09:17:37.12 Intake.select :open @09:17:39.28 Intake @15.0psia, vacuum from 8.5 to 12.5psi @09:17:39.30 SS.reconfigure forSampling @09:17:39.36 SS.seek 25.00ml @09:17:48.68 delay 3 seconds @09:17:51.69 ATV.dial ASV,via => air2 @09:17:52.77 Stroke completed -- waiting for pressure to equalize ... @09:17:53.40 ASV.dial :SPEelution @09:17:53.48 Intake.select :close @09:17:53.62 SSV.dial :exhaust @09:17:53.93 AFV.select :carrierSolution @09:17:54.08 AS.reconfigure ASnorm @09:17:54.12 AS.seek 0.759ml @09:17:54.41 Exhaust.select :open @09:17:54.52 SS.reconfigure SSexhale @09:17:54.57 SS.seek 0.00ml @09:18:03.26 Exhaust.select :close @09:18:03.36 SSV.dial CBV @09:18:03.38 CBV.dial SSV @09:18:04.68 Allowing 185s for 25.0ml of remaining 925.0ml @09:18:04.69 Intake.select :open @09:18:06.85 Intake @15.1psia, vacuum from 8.6 to 12.6psi @09:18:06.86 SS.reconfigure forSampling @09:18:06.92 SS.seek 25.00ml @09:18:20.57 Stroke completed -- waiting for pressure to equalize ... @09:18:21.21 Intake.select :close @09:18:21.35 SSV.dial :exhaust @09:18:22.12 Exhaust.select :open @09:18:22.23 SS.reconfigure SSexhale @09:18:22.29 SS.seek 0.00ml @09:18:27.65 delay 3 seconds @09:18:30.67 AFV.select :holdingCoil @09:18:30.82 AS.reconfigure ASslow1 @09:18:30.86 AS.seek 0.489ml @09:18:31.20 Exhaust.select :close @09:18:31.31 SSV.dial CBV @09:18:31.33 CBV.dial SSV @09:18:32.13 Allowing 185s for 25.0ml of remaining 900.0ml @09:18:32.14 Intake.select :open @09:18:34.29 Intake @15.3psia, vacuum from 8.8 to 12.8psi @09:18:34.31 SS.reconfigure forSampling @09:18:34.37 SS.seek 25.00ml @09:18:48.16 Stroke completed -- waiting for pressure to equalize ... @09:18:48.80 Intake.select :close @09:18:48.95 SSV.dial :exhaust @09:18:49.73 Exhaust.select :open @09:18:49.85 SS.reconfigure SSexhale @09:18:49.90 SS.seek 0.00ml @09:18:59.15 Exhaust.select :close @09:18:59.26 SSV.dial CBV @09:18:59.28 CBV.dial SSV @09:19:00.07 Allowing 185s for 25.0ml of remaining 875.0ml @09:19:00.09 Intake.select :open @09:19:02.23 Intake @15.3psia, vacuum from 8.8 to 12.8psi @09:19:02.25 SS.reconfigure forSampling @09:19:02.31 SS.seek 25.00ml @09:19:16.44 Stroke completed -- waiting for pressure to equalize ... @09:19:17.08 Intake.select :close @09:19:17.22 SSV.dial :exhaust @09:19:17.99 Exhaust.select :open @09:19:18.10 SS.reconfigure SSexhale @09:19:18.15 SS.seek 0.00ml @09:19:26.18 delay 5 seconds @09:19:27.12 Exhaust.select :close @09:19:27.22 SSV.dial CBV @09:19:27.24 CBV.dial SSV @09:19:28.03 Allowing 185s for 25.0ml of remaining 850.0ml @09:19:28.04 Intake.select :open @09:19:30.20 Intake @15.3psia, vacuum from 8.8 to 12.8psi @09:19:30.22 SS.reconfigure forSampling @09:19:30.28 SS.seek 25.00ml @09:19:31.24 AS.reconfigure ASslow2 @09:19:31.28 AS.seek 0.454ml @09:19:44.13 Stroke completed -- waiting for pressure to equalize ... @09:19:44.77 Intake.select :close @09:19:44.91 SSV.dial :exhaust @09:19:45.68 Exhaust.select :open @09:19:45.79 SS.reconfigure SSexhale @09:19:45.84 SS.seek 0.00ml @09:19:54.61 Exhaust.select :close @09:19:54.71 SSV.dial CBV @09:19:54.73 CBV.dial SSV @09:19:55.51 Allowing 185s for 25.0ml of remaining 825.0ml @09:19:55.53 Intake.select :open @09:19:57.68 Intake @15.3psia, vacuum from 8.8 to 12.8psi @09:19:57.70 SS.reconfigure forSampling @09:19:57.76 SS.seek 25.00ml @09:20:07.08 delay 1:00 @09:20:11.64 Stroke completed -- waiting for pressure to equalize ... @09:20:12.29 Intake.select :close @09:20:12.43 SSV.dial :exhaust @09:20:13.22 Exhaust.select :open @09:20:13.33 SS.reconfigure SSexhale @09:20:13.38 SS.seek 0.00ml @09:20:22.16 Exhaust.select :close @09:20:22.26 SSV.dial CBV @09:20:22.28 CBV.dial SSV @09:20:23.08 Allowing 185s for 25.0ml of remaining 800.0ml @09:20:23.09 Intake.select :open @09:20:25.24 Intake @15.3psia, vacuum from 8.8 to 12.8psi @09:20:25.26 SS.reconfigure forSampling @09:20:25.32 SS.seek 25.00ml @09:20:39.24 Stroke completed -- waiting for pressure to equalize ... @09:20:39.89 Intake.select :close @09:20:40.03 SSV.dial :exhaust @09:20:40.80 Exhaust.select :open @09:20:40.91 SS.reconfigure SSexhale @09:20:40.96 SS.seek 0.00ml @09:20:49.69 Exhaust.select :close @09:20:49.79 SSV.dial CBV @09:20:49.81 CBV.dial SSV @09:20:50.61 Allowing 185s for 25.0ml of remaining 775.0ml @09:20:50.62 Intake.select :open @09:20:52.77 Intake @15.5psia, vacuum from 9.0 to 13.0psi @09:20:52.79 SS.reconfigure forSampling @09:20:52.85 SS.seek 25.00ml @09:21:06.77 Stroke completed -- waiting for pressure to equalize ... @09:21:07.10 pushing eluate to holding coil @09:21:07.16 AS.seek 0.294ml @09:21:07.95 Intake.select :close @09:21:08.09 SSV.dial :exhaust @09:21:08.86 Exhaust.select :open @09:21:08.98 SS.reconfigure SSexhale @09:21:09.04 SS.seek 0.00ml @09:21:17.78 Exhaust.select :close @09:21:17.88 SSV.dial CBV @09:21:17.90 CBV.dial SSV @09:21:18.70 Allowing 185s for 25.0ml of remaining 750.0ml @09:21:18.71 Intake.select :open @09:21:20.86 Intake @15.4psia, vacuum from 8.9 to 12.9psi @09:21:20.88 SS.reconfigure forSampling @09:21:20.93 SS.seek 25.00ml @09:21:34.75 Stroke completed -- waiting for pressure to equalize ... @09:21:35.40 Intake.select :close @09:21:35.55 SSV.dial :exhaust @09:21:36.31 Exhaust.select :open @09:21:36.42 SS.reconfigure SSexhale @09:21:36.47 SS.seek 0.00ml @09:21:45.19 Exhaust.select :close @09:21:45.29 SSV.dial CBV @09:21:45.31 CBV.dial SSV @09:21:46.09 Allowing 185s for 25.0ml of remaining 725.0ml @09:21:46.11 Intake.select :open @09:21:48.27 Intake @15.5psia, vacuum from 9.0 to 13.0psi @09:21:48.29 SS.reconfigure forSampling @09:21:48.35 SS.seek 25.00ml @09:22:02.31 Stroke completed -- waiting for pressure to equalize ... @09:22:02.97 Intake.select :close @09:22:03.13 SSV.dial :exhaust @09:22:03.89 Exhaust.select :open @09:22:04.00 SS.reconfigure SSexhale @09:22:04.05 SS.seek 0.00ml @09:22:12.81 Exhaust.select :close @09:22:12.91 SSV.dial CBV @09:22:12.93 CBV.dial SSV @09:22:13.72 Allowing 185s for 25.0ml of remaining 700.0ml @09:22:13.74 Intake.select :open @09:22:15.89 Intake @15.5psia, vacuum from 9.0 to 13.0psi @09:22:15.91 SS.reconfigure forSampling @09:22:15.97 SS.seek 25.00ml @09:22:29.83 Stroke completed -- waiting for pressure to equalize ... @09:22:30.48 Intake.select :close @09:22:30.62 SSV.dial :exhaust @09:22:31.39 Exhaust.select :open @09:22:31.50 SS.reconfigure SSexhale @09:22:31.55 SS.seek 0.00ml @09:22:40.50 Exhaust.select :close @09:22:40.60 SSV.dial CBV @09:22:40.62 CBV.dial SSV @09:22:41.40 Allowing 185s for 25.0ml of remaining 675.0ml @09:22:41.42 Intake.select :open @09:22:43.57 Intake @15.5psia, vacuum from 9.0 to 13.0psi @09:22:43.59 SS.reconfigure forSampling @09:22:43.65 SS.seek 25.00ml @09:22:47.91 Vacuum too high -- waiting up to 40s @09:23:28.00 SS.reconfigure SSconfig @09:23:28.05 SS.hold @09:23:28.16 Intake.select :close @09:23:28.32 ShallowSampler::Clogged in WCR -- Intake @15.6psia, & Outlet @2.6psia -- no progress after 330.7ml @09:23:28.33 SSV.dial :exhaust @09:23:29.10 Exhaust.select :open @09:23:29.21 SS.reconfigure SSexhale @09:23:29.26 SS.seek 0.00ml @09:23:32.71 Exhaust.select :close @09:23:32.83 Sampled 330.7ml @09:23:32.84 collectSample: Complete @09:23:32.85 Drying puck before fixing for 04:00.00 @09:23:32.87 CBV.dial :waste2b @09:23:33.63 delay 1:00 @09:23:35.82 Sleepy.power :sampler,:OFF @09:23:50.62 delay 30 seconds @09:24:20.63 ATV.dial :waste,via => air3 @09:24:22.10 AS.reconfigure ASconfig @09:24:22.14 AS.seek empty @09:24:33.63 CSV.dial :air @09:24:34.08 CS.reconfigure CSfast @09:24:34.13 CS.seek 10.000ml @09:24:39.65 Parking Bleach in LysateSource line and SPEcolumn @09:24:39.66 ATV.dial :air3 @09:24:40.25 AS.reconfigure ASslow1 @09:24:40.29 AS.seek 0.029ml @09:24:46.73 ATV.dial :ElutionWater @09:24:47.22 AS.seek 0.059ml @09:24:53.59 ATV.dial :air3 @09:24:53.98 AS.seek 0.069ml @09:24:56.31 delay 2 seconds @09:24:58.04 CBV.dial between CSV and waste2b @09:24:58.32 ATV.dial :bleach @09:24:58.38 Pumping wcr puck from tube 4 up to 55psi... @09:24:58.45 CBV.dial SSV @09:24:58.71 AS.seek 0.709ml @09:24:59.89 CBV.dial between CSV and waste2b @09:25:00.86 CSV.dial between air and CTV @09:25:01.27 CS.seek 6.765ml @09:25:09.71 CTV.dial CSV,avoiding => air @09:25:09.73 CSV.dial CTV @09:25:10.50 CS.reconfigure CSpressurize @09:25:10.55 CS.seek 0.000ml @09:25:28.29 CSV.dial :air @09:25:29.03 CS.reconfigure CSfast @09:25:29.08 CS.seek 10.000ml @09:25:52.87 CSV.dial between air and CTV @09:25:53.17 CS.seek 2.560ml @09:26:10.83 CTV.dial CSV,avoiding => air @09:26:10.84 CSV.dial CTV @09:26:11.54 CS.reconfigure CSpressurize @09:26:11.59 CS.seek 0.000ml @09:26:15.31 CBV.dial :waste2b @09:26:15.64 CS.reconfigure CSconfig @09:26:15.69 CS.seek empty @09:26:19.69 delay 1:00 @09:27:09.49 delay 3 seconds @09:27:12.50 ATV.dial :air3 @09:27:12.99 delay 3 seconds @09:27:16.05 AS.seek 0.734ml @09:27:19.70 CTV.dial :air,avoiding => intake @09:27:20.98 Fix #1 of 2 (with rnal) for 02:00.00 @09:27:20.99 CS Pulling 2ml of rnal @09:27:21.01 CSV.dial CSR @09:27:21.44 delay 3 seconds @09:27:21.48 CSR.select :rnal @09:27:21.59 CS.seek 2.000ml @09:27:24.46 ATV.dial :bleach @09:27:24.85 AS.seek 0.719ml @09:27:27.53 delay 5 seconds @09:27:28.14 delay 3 seconds @09:27:31.15 ATV.dial :AIV @09:27:32.25 AS.reconfigure ASconfig @09:27:32.29 AS.seek 0.015ml @09:27:32.54 CSR.select :air @09:27:32.65 CS.reconfigure CSfast @09:27:32.70 CS.seek 10.000ml @09:27:51.78 CBV.dial between CSV and waste2b @09:27:52.12 Pumping wcr puck from tube 4 up to 55psi... @09:27:52.18 CBV.dial SSV @09:27:53.73 CBV.dial between CSV and waste2b @09:27:54.69 CSV.dial between air and CTV @09:27:55.36 CS.seek 6.749ml @09:28:03.70 CTV.dial CSV,avoiding => air @09:28:03.72 CSV.dial CTV @09:28:05.06 CS.reconfigure CSpressurize @09:28:05.11 CS.seek 0.000ml @09:28:13.63 ATV.dial :waste @09:28:14.93 AS.seek empty @09:28:16.13 ATV.dial :air3 @09:28:16.80 AS.reconfigure ASslow1 @09:28:16.84 AS.seek 0.009ml @09:28:19.17 delay 2 seconds @09:28:21.18 ATV.dial :bleach @09:28:21.58 AS.seek 0.579ml @09:28:22.49 delay 2:00 @09:30:18.08 delay 3 seconds @09:30:21.09 ATV.dial :air3 @09:30:21.45 delay 3 seconds @09:30:22.50 CBV.dial :waste2b @09:30:22.83 CS.reconfigure CSconfig @09:30:22.88 CS.seek empty @09:30:23.59 delay 1:00 @09:30:24.50 AS.seek 0.604ml @09:30:29.84 delay 3 seconds @09:30:32.85 ATV.dial :bleach @09:30:33.25 AS.seek 0.589ml @09:30:36.59 delay 3 seconds @09:30:39.60 ATV.dial :ASV @09:30:40.50 ASV.dial :wasteR @09:30:41.18 AS.seek 0.000ml @09:31:23.60 CTV.dial :air,avoiding => intake @09:31:24.89 Fix #2 of 2 (with rnal) for 02:00.00 @09:31:24.90 CS Pulling 2ml of rnal @09:31:24.91 CSV.dial CSR @09:31:25.35 CSR.select :rnal @09:31:25.47 CS.seek 2.000ml @09:31:31.34 delay 5 seconds @09:31:36.35 CSR.select :air @09:31:36.46 CS.reconfigure CSfast @09:31:36.51 CS.seek 10.000ml @09:31:55.58 CBV.dial between CSV and waste2b @09:31:55.92 Pumping wcr puck from tube 4 up to 55psi... @09:31:55.98 CBV.dial SSV @09:31:57.44 CBV.dial between CSV and waste2b @09:31:58.41 CSV.dial between air and CTV @09:31:59.09 CS.seek 6.610ml @09:32:07.69 CTV.dial CSV,avoiding => air @09:32:07.71 CSV.dial CTV @09:32:09.03 CS.reconfigure CSpressurize @09:32:09.08 CS.seek 0.000ml @09:32:25.45 delay 2:00 @09:32:41.59 delay 1:00 @09:33:41.60 AFV.select :carrierSolution @09:33:41.70 delay 2 seconds @09:33:43.71 AS.reconfigure ASconfig @09:33:43.75 AS.seek 1.000ml @09:34:25.46 CBV.dial :waste2b @09:34:25.79 CS.reconfigure CSconfig @09:34:25.84 CS.seek empty @09:34:27.45 delay 1:00 @09:34:42.35 delay 1 second @09:34:43.36 AFV.select :holdingCoil @09:34:43.46 delay 1 second @09:34:44.47 ATV.dial :ASV @09:34:44.48 ASV.dial :wasteR @09:34:44.49 AS.reconfigure ASslow1 @09:34:44.54 AS.seek 0.000ml @09:35:27.47 CTV.dial :air,avoiding => intake @09:35:28.81 Storing wcr puck in Collection Clamp to tube 3 @09:35:28.93 SE.seek down @09:35:29.27 SC.reconfigure SCtight @09:35:29.32 SC.seek 3 @09:35:31.53 SC.reconfigure SCloose @09:35:31.67 SE.reconfigure SEplace @09:35:31.74 Elbow.reconfigure TightElbow @09:35:31.79 Elbow.seek CC @09:35:31.84 SE.seek beamBreak @09:35:31.95 Elbow.reconfigure LooseElbow @09:35:32.05 Forearm.seek CC @09:35:32.99 CC.seek sealed @09:35:33.93 Hand.grip @09:35:34.64 CC.seek open @09:35:34.75 SE.configure SEconfig @09:35:34.80 SE.seek place @09:35:38.02 Forearm.seek retract @09:35:38.98 Elbow.reconfigure TightElbow @09:35:39.03 Elbow.seek 3 @09:35:40.67 Elbow.reconfigure LimpElbow @09:35:40.73 Forearm.seek 3 @09:35:41.72 Elbow.hold @09:35:41.79 Elbow.reconfigure TightElbow @09:35:41.84 Hand.release @09:35:42.72 Forearm.coast @09:35:42.77 Forearm.seek 3 + 345 counts @09:35:43.06 Forearm.reconfigure TightForearm @09:35:43.12 Forearm.seek 3 + 800 counts @09:35:43.52 SE.seek down @09:35:44.62 Forearm.reconfigure LooseForearm @09:35:44.67 Forearm.seek retract @09:35:46.25 SE.coast @09:35:46.29 SC.coast @09:35:46.34 Starting finalPurge @09:35:46.35 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @09:35:46.51 Elbow.seek FlushPuck::Garage @09:35:48.21 Elbow.reconfigure LooseElbow @09:35:48.32 Forearm.seek garage @09:35:49.38 Hand.grip @09:35:50.23 Forearm.seek retract @09:35:51.28 Elbow.reconfigure TightElbow @09:35:51.32 Elbow.seek CC @09:35:54.12 Elbow.reconfigure LooseElbow @09:35:54.21 Forearm.reconfigure TightForearm @09:35:54.26 Forearm.seek CC @09:35:55.40 CC.seek closed @09:35:59.15 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 2.05 @09:35:59.24 Hand.release @09:36:00.64 Forearm.reconfigure LooseForearm @09:36:00.69 Forearm.seek retract @09:36:01.70 Flushing the Collection Valve::Manifold @09:36:01.81 CS Pulling 5ml of flush @09:36:01.82 CSV.dial CSR @09:36:02.27 CSR.select :flush @09:36:02.40 CS.seek 5.000ml @09:36:15.93 delay 5 seconds @09:36:20.95 CSR.select :air @09:36:21.11 CS.reconfigure CSfast @09:36:21.16 CS.seek 7.501ml @09:36:27.71 CSV.dial :waste @09:36:29.20 CS.reconfigure CSconfig @09:36:29.24 CS.seek 0.000ml @09:36:49.21 Flushing lower manifold's waste1 port @09:36:49.33 CSV.dial :flush @09:36:49.87 CS.reconfigure CSfast @09:36:49.91 CS.seek 5.000ml @09:37:02.08 delay 5 seconds @09:37:07.10 CSV.dial :air @09:37:07.58 CS.seek 7.000ml @09:37:13.00 CSV.dial CTV @09:37:13.02
CTV.dial CSV @09:37:14.35 CBV.dial :waste1 @09:37:15.12 CS.seek 0.000ml @09:37:31.75 Flushing lower manifold's waste2b port @09:37:31.86 CSV.dial :flush @09:37:32.68 CS.seek 5.000ml @09:37:44.86 delay 5 seconds @09:37:49.88 CSV.dial :air @09:37:50.46 CS.seek 6.999ml @09:37:55.87 CSV.dial CTV @09:37:55.89
CTV.dial CSV @09:37:56.34 CBV.dial :waste2b @09:37:57.09 CS.seek 0.000ml @09:38:09.49 delay 20 seconds @09:38:13.77
Flushing upper manifold @09:38:13.89 CSV.dial :flush @09:38:14.71 CS.seek 5.000ml @09:38:26.92 delay 5 seconds @09:38:29.51 ATV.dial :air3 @09:38:30.64 AS.seek 0.590ml @09:38:31.93
CSV.dial :air @09:38:32.53 CS.seek 7.000ml @09:38:37.90 CSV.dial CBV @09:38:37.92
CBV.dial CSV @09:38:39.06 CTV.dial :air @09:38:40.35 CS.seek 0.000ml @09:38:56.95 delay 7 seconds @09:39:03.96 CS.seek 3.000ml @09:39:11.61 CSV.dial :waste @09:39:12.13 CS.seek 0.000ml @09:39:19.79 CSV.dial :flush @09:39:20.24 CS.seek 3.000ml @09:39:27.89 delay 5 seconds @09:39:32.90 CSV.dial :air @09:39:33.41 CS.seek 5.000ml @09:39:38.85 CSV.dial :air @09:39:38.87 CS.seek 0.000ml @09:39:51.04 delay 7 seconds @09:39:58.05 CS.seek 3.000ml @09:40:05.73 CSV.dial :waste @09:40:06.55 CS.seek 0.000ml @09:40:14.23 Disinfecting external Intake with mfbkill @09:40:14.35 CSV.dial CSR @09:40:15.63 CSR.select :mfbkill @09:40:15.75 CS.seek 8.000ml @09:40:31.22 delay 3 seconds @09:40:34.24 ATV.dial :ASV @09:40:34.79
delay 5 seconds @09:40:35.32 ASV.dial :wasteR @09:40:35.39 AS.seek 0.090ml @09:40:39.80
CSR.select :air @09:40:39.90 CS.seek 10.000ml @09:40:45.29 CSV.dial CBV @09:40:45.32
CBV.dial CSV @09:40:46.40 CTV.dial :intake @09:40:47.12 Intake.select :open @09:40:47.23 CS.seek 2.000ml @09:41:06.38 Intake.select :close @09:41:06.49 CTV.dial :air @09:41:07.24 CS.reconfigure CSconfig @09:41:07.28 CS.seek 0.000ml @09:41:13.19 Flushing lower manifold's waste1 port @09:41:13.20 CS Pulling 4ml of mfbkill @09:41:13.22 CSV.dial CSR @09:41:14.30 CSR.select :mfbkill @09:41:15.00 CS.seek 4.000ml @09:41:25.91 delay 5 seconds @09:41:30.93 CSR.select :air @09:41:31.03 CS.reconfigure CSfast @09:41:31.08 CS.seek 10.000ml @09:41:45.52 CSV.dial CTV @09:41:45.54
CTV.dial CSV @09:41:46.88 CBV.dial :waste1 @09:41:47.37 CS.seek 0.000ml @09:42:10.76 Flushing lower manifold's waste2b port CS Pulling 4ml of mfbkill @09:42:10.78 CSV.dial CSR @09:42:11.30 CSR.select :mfbkill @09:42:11.42 CS.reconfigure CSconfig @09:42:11.47 CS.seek 4.000ml @09:42:17.56 delay 30 seconds @09:42:22.44
delay 5 seconds @09:42:27.45 CSR.select :air @09:42:27.55 CS.reconfigure CSfast @09:42:27.60 CS.seek 10.000ml @09:42:42.01 CSV.dial CTV @09:42:42.03
CTV.dial CSV @09:42:42.56 CBV.dial :waste2b @09:42:43.33 CS.seek 0.000ml @09:42:47.57 ATV.dial :extSrc @09:42:48.20 delay 3 seconds @09:42:51.21 ATV.dial :ElutionWater @09:42:52.16 AS.seek 0.120ml @09:42:58.62 ATV.dial :air3 @09:42:59.02 AS.seek 0.130ml @09:43:01.33 delay 2 seconds @09:43:03.34 ATV.dial :bleach @09:43:03.72 AS.seek 0.230ml @09:43:06.47
Killing upper manifold @09:43:06.48 CS Pulling 4ml of mfbkill @09:43:06.50 CSV.dial CSR @09:43:07.02 CSR.select :mfbkill @09:43:07.13 CS.reconfigure CSconfig @09:43:07.18 CS.seek 4.000ml @09:43:18.10 delay 5 seconds @09:43:23.11 CSR.select :air @09:43:23.21 CS.reconfigure CSfast @09:43:23.27 CS.seek 10.000ml @09:43:24.37 delay 3 seconds @09:43:27.38 ATV.dial :air3 @09:43:27.73 delay 3 seconds @09:43:30.79 AS.seek 0.255ml @09:43:36.15 delay 3 seconds @09:43:37.67 CSV.dial CBV @09:43:37.69
CBV.dial CSV @09:43:38.71 CTV.dial :air @09:43:39.16 ATV.dial :bleach @09:43:39.56 AS.seek 0.240ml @09:43:40.01
CS.seek 0.000ml @09:43:42.87 delay 3 seconds @09:43:45.88 ATV.dial :ASV @09:43:46.87 ASV.dial :SPEdelivery @09:43:47.64 AFV.select :carrierSolution @09:43:47.74 delay 2 seconds @09:43:49.75 AS.reconfigure ASconfig @09:43:49.80 AS.seek 0.500ml @09:44:03.06
delay 7 seconds @09:44:05.23 delay 1 second @09:44:06.24 AFV.select :holdingCoil @09:44:06.39 AS.reconfigure ASslow1 @09:44:06.43 AS.seek 0.170ml @09:44:06.83 Session Ended @09:44:10.07
CS.seek 3.000ml @09:44:17.65 CSV.dial :waste @09:44:18.10 CS.seek 0.000ml @09:44:25.62 Flushing upper manifold @09:44:25.72 CSV.dial :flush @09:44:26.15 CS.seek 5.000ml @09:44:38.31 delay 5 seconds @09:44:43.32 CSV.dial :air @09:44:43.80 CS.seek 7.000ml @09:44:49.22 CSV.dial CBV @09:44:49.24
CBV.dial CSV @09:44:50.31 CTV.dial :air @09:44:50.33 CS.seek 0.000ml @09:45:06.75 delay 7 seconds @09:45:13.76 CS.seek 3.000ml @09:45:14.00 SPE.hold 2:46:40,95.0C @09:45:14.02 delay 3:00 @09:45:21.56
CSV.dial :waste @09:45:22.08 CS.seek 0.000ml @09:45:30.02 CSV.dial :flush @09:45:30.46 CS.seek 3.000ml @09:45:38.31 delay 5 seconds @09:45:43.32 CSV.dial :air @09:45:43.81 CS.seek 4.999ml @09:45:49.40 CSV.dial :air @09:45:49.42 CS.seek 0.000ml @09:46:01.84 delay 7 seconds @09:46:08.85 CS.seek 3.000ml @09:46:16.77 CSV.dial :waste @09:46:17.60 CS.seek 0.000ml @09:46:25.46 Flushing lower manifold's waste1 port @09:46:26.12 CSV.dial :flush @09:46:26.64 CS.seek 5.000ml @09:46:39.05 delay 5 seconds @09:46:44.06 CSV.dial :air @09:46:44.54 CS.seek 7.000ml @09:46:50.06 CSV.dial CTV @09:46:50.08
CTV.dial CSV @09:46:51.43 CBV.dial :waste1 @09:46:51.89 CS.seek 0.000ml @09:47:08.80 Flushing lower manifold's waste2b port @09:47:08.90 CSV.dial :flush @09:47:09.72 CS.seek 5.000ml @09:47:22.18 delay 5 seconds @09:47:27.19 CSV.dial :air @09:47:27.76 CS.seek 7.000ml @09:47:33.28 CSV.dial CTV @09:47:33.29
CTV.dial CSV @09:47:33.75 CBV.dial :waste2b @09:47:34.52 CS.seek 0.000ml @09:47:51.52 Disinfecting external Intake with kill @09:47:51.62 CSV.dial CSR @09:47:52.05 CSR.select :kill @09:47:52.15 CS.seek 8.000ml @09:48:11.61 delay 5 seconds @09:48:14.03 AFV.select :carrierSolution @09:48:14.12 delay 2 seconds @09:48:16.13 AS.reconfigure ASconfig @09:48:16.16 AS.seek 1.000ml @09:48:16.62
CSR.select :air @09:48:16.71 CS.seek 10.000ml @09:48:22.15 CSV.dial CBV @09:48:22.16
CBV.dial CSV @09:48:23.21 CTV.dial :intake @09:48:23.94 Intake.select :open @09:48:24.04 CS.seek 2.000ml @09:48:43.46 Intake.select :close @09:48:43.55 CTV.dial :air @09:48:44.29 CS.reconfigure CSconfig @09:48:44.32 CS.seek 0.000ml @09:48:50.21 CSV.dial CSR @09:48:51.30 CSR.select :flush @09:48:51.40 CS.seek 5.000ml @09:49:04.83 delay 1 second @09:49:04.89
delay 5 seconds @09:49:05.84 AFV.select :holdingCoil @09:49:05.93 delay 1 second @09:49:06.94 ASV.dial :wasteD @09:49:08.08 AS.seek 0.000ml @09:49:09.90
CSR.select :air @09:49:09.99 CS.seek 10.000ml @09:49:23.55 CSV.dial CBV @09:49:24.62 CS.seek 0.000ml @09:49:50.77 Garaging FlushPuck in Collection Clamp @09:49:50.83 Elbow.reconfigure TightElbow @09:49:50.86 Elbow.seek CC @09:49:50.96 Elbow.reconfigure LooseElbow @09:49:51.04 Forearm.seek CC @09:49:51.99 CC.seek sealed @09:49:52.95 Hand.grip @09:49:53.73 CC.seek open @09:49:57.05 Forearm.seek retract @09:49:58.01 Elbow.reconfigure TightElbow @09:49:58.04 Elbow.seek garage @09:50:00.85 Elbow.reconfigure LooseElbow @09:50:00.93 Forearm.reconfigure TightForearm @09:50:00.96 Forearm.seek garage @09:50:02.16 Hand.release @09:50:02.89 Forearm.reconfigure LooseForearm @09:50:02.92 Forearm.seek retract @09:50:06.64 Priming Eluate @09:50:06.65 AFV.select :carrierSolution @09:50:06.74 delay 2 seconds @09:50:08.75 AS.seek 0.500ml @09:50:38.17 delay 1 second @09:50:39.18 AFV.select :holdingCoil @09:50:39.27 ATV.dial ASV @09:50:39.28 ASV.dial :SPErecovery @09:50:39.78 Swish #1 of 2 @09:50:39.84 AS.reconfigure ASslow0 @09:50:39.87 AS.seek 0.600ml @09:50:50.38 AS.seek 0.500ml @09:51:00.86 Swish #2 of 2 @09:51:00.92 AS.seek 0.600ml @09:51:11.50 AS.seek 0.500ml @09:51:21.98 delay 3 seconds @09:51:25.04 AS.reconfigure ASslow1 @09:51:25.07 AS.seek 0.676ml @09:52:01.23 delay 3 seconds @09:52:04.25 Eluate primed @09:52:04.26 ATV.dial :waste2 @09:52:05.36 AS.reconfigure ASnorm @09:52:05.39 AS.seek 0.000ml @09:52:40.21 SPE.coast @09:52:40.29 ASV.dial :SPErecovery @09:52:40.31 Setting up PCR for HF183em -- writing /var/log/esp/HF183em14mar1909h1ml.pcr @09:52:40.32 Building PCR Slug from ASV with MM2 mix, PP4 primer @09:52:40.33 Priming with carrier ATV.dial ARV @09:52:41.04 ARV.dial :carrier @09:52:41.32 AS.seek 0.500ml @09:53:07.18 AFV.select :carrierSolution @09:53:07.28 delay 2 seconds @09:53:09.29 AS.reconfigure ASconfig @09:53:09.33 AS.seek 1.000ml @09:53:38.74 delay 1 second @09:53:39.75 AFV.select :holdingCoil @09:53:39.84 delay 1 second @09:53:40.85 ATV.dial :waste @09:53:42.12 AS.reconfigure ASnorm @09:53:42.15 AS.seek empty @09:54:33.63 AFV.select :carrierSolution @09:54:33.73 delay 2 seconds @09:54:35.74 AS.reconfigure ASconfig @09:54:35.78 AS.seek 0.500ml @09:55:05.27 delay 1 second @09:55:06.28 AFV.select :holdingCoil @09:55:06.37 Building Slug @09:55:06.38 ATV.dial :ARV @09:55:07.64 delay 2 seconds @09:55:09.65 ARV.dial :air1 @09:55:09.97 delay 2 seconds @09:55:12.03 AS.reconfigure ASslow2 @09:55:12.06 AS.seek 0.506ml @09:55:18.50 delay 2 seconds @09:55:20.51 ARV.dial :MM2 @09:55:21.74 delay 2 seconds @09:55:23.75 Adding MM2 @09:55:23.81 AS.seek 0.524ml @09:55:42.59 delay 25 seconds @09:56:07.60 ARV.dial :PP4 @09:56:07.93 Adding PP4 @09:56:07.94 delay 2 seconds @09:56:09.99 AS.seek 0.530ml @09:56:16.48 delay 10 seconds @09:56:26.49 ARV.dial :air3 @09:56:26.80 adding ARV air @09:56:26.81 delay 2 seconds @09:56:28.87 AS.seek 0.536ml @09:56:35.23 delay 5 seconds @09:56:40.24 ARV.dial :carrier @09:56:41.26 delay 7 seconds @09:56:48.32 AS.seek 0.594ml @09:57:47.70 delay 5 seconds @09:57:52.71 ATV.dial :ASV @09:57:53.04 delay 2 seconds @09:57:55.10 AS.seek 0.600ml @09:58:01.59 delay 4 seconds @09:58:05.60 ATV.dial ARV @09:58:06.04 delay 2 seconds @09:58:08.05 ARV.dial :carrier @09:58:08.06 delay 2 seconds @09:58:10.11 AS.seek 0.650ml @09:59:01.39 delay 4 seconds @09:59:05.40 ATV.dial PCR @09:59:05.92 ASV.dial :waste1 @09:59:06.60 ARV.dial :air1 @09:59:06.94 Positioning Slug by 0.324ml @09:59:06.99 AS.seek 0.326ml @10:04:37.64 delay 5 seconds @10:04:42.65 ATV.dial ARV @10:04:43.17 ASV.dial :PCRlock @10:04:43.50 delay 5 seconds @10:04:48.51 Running HF183em ProfileEM60C2 thermocycling @10:04:48.52 PCR.program ProfileEM60C2 @10:04:48.63 ProfileEM60C2 should complete in < 2:11:38 @10:04:48.64 PCR.run 2:49:32.5 @12:00:49.12 PCR.off @12:00:49.65 PCR for HF183em completed -- closed /var/log/esp/HF183em14mar1909h1ml.pcr @12:00:49.70 Cleaning PCR @12:00:49.71 AFV.select :carrierSolution @12:00:49.80 delay 2 seconds @12:00:51.81 AS.reconfigure ASconfig @12:00:51.84 AS.seek 0.500ml @12:01:02.27 delay 1 second @12:01:03.28 AFV.select :holdingCoil @12:01:03.37 ASV.dial :waste1 @12:01:04.12 ATV.dial :air3 @12:01:05.06 AS.reconfigure ASslow1 @12:01:05.09 AS.seek 0.510ml @12:01:07.41 delay 2 seconds @12:01:09.42 ATV.dial :bleach @12:01:09.90 AS.seek 0.810ml @12:02:11.32 delay 3 seconds @12:02:14.33 ATV.dial :air3 @12:02:14.66 delay 3 seconds @12:02:17.72 AS.seek 0.835ml @12:02:23.07 delay 3 seconds @12:02:26.08 ATV.dial :bleach @12:02:26.57 AS.seek 0.820ml @12:02:29.89 delay 3 seconds @12:02:32.90 ARV.dial :waste @12:02:33.39 delay 3 seconds @12:02:36.40 ATV.dial :ARV @12:02:37.15 AS.reconfigure ASconfig @12:02:37.18 AS.seek 0.470ml @12:02:57.86 AFV.select :carrierSolution @12:02:57.95 delay 2 seconds @12:02:59.96 AS.seek 1.000ml @12:03:31.17 delay 1 second @12:03:32.18 AFV.select :holdingCoil @12:03:32.27 delay 1 second @12:03:33.28 AS.reconfigure ASfast @12:03:33.31 AS.seek 0.000ml @12:04:01.06 AFV.select :carrierSolution @12:04:01.16 delay 2 seconds @12:04:03.17 AS.reconfigure ASconfig @12:04:03.20 AS.seek 1.000ml @12:05:01.80 delay 1 second @12:05:02.81 AFV.select :holdingCoil @12:05:02.90 delay 1 second @12:05:03.91 AS.reconfigure ASfast @12:05:03.94 AS.seek 0.000ml @12:05:31.67 AFV.select :carrierSolution @12:05:31.77 delay 2 seconds @12:05:33.78 AS.reconfigure ASconfig @12:05:33.81 AS.seek 1.000ml @12:06:32.42 delay 1 second @12:06:33.43 AFV.select :holdingCoil @12:06:33.52 delay 1 second @12:06:34.53 AS.reconfigure ASfast @12:06:34.56 AS.seek 0.000ml @12:07:02.25 ARV.dial between carrier and air1 @12:07:02.67 ATV.dial :air3 @12:07:03.69 AS.reconfigure ASslow1 @12:07:03.73 AS.seek 0.010ml @12:07:06.06 delay 2 seconds @12:07:08.07 ATV.dial :bleach @12:07:08.47 AS.seek 0.810ml @12:09:51.87 delay 3 seconds @12:09:54.88 ATV.dial :air3 @12:09:55.22 delay 3 seconds @12:09:58.28 AS.seek 0.835ml @12:10:03.61 delay 3 seconds @12:10:06.62 ATV.dial :bleach @12:10:07.11 AS.seek 0.820ml @12:10:10.44 delay 3 seconds @12:10:13.45 ATV.dial PCR @12:10:13.79 AS.reconfigure ASconfig @12:10:13.82 AS.seek empty @12:11:01.95 PCR.program :bleachProfile @12:11:02.00 PCR program should complete in < 2:45 @12:11:02.01 PCR.run 10:00 @12:14:00.20 PCR.off @12:14:00.23 AFV.select :carrierSolution @12:14:00.32 delay 2 seconds @12:14:02.33 AS.seek 1.000ml @12:15:00.97 delay 1 second @12:15:01.98 AFV.select :holdingCoil @12:15:02.07 delay 1 second @12:15:03.08 AS.reconfigure ASfast @12:15:03.11 AS.seek 0.000ml @12:15:30.85 delay 3 seconds @12:15:33.86 AFV.select :carrierSolution @12:15:33.95 delay 2 seconds @12:15:35.96 AS.reconfigure ASconfig @12:15:35.99 AS.seek 1.000ml @12:16:34.56 delay 1 second @12:16:35.57 AFV.select :holdingCoil @12:16:35.66 delay 1 second @12:16:36.67 AS.reconfigure ASfast @12:16:36.70 AS.seek 0.000ml @12:17:04.98 delay 3 seconds @12:17:07.99 AFV.select :carrierSolution @12:17:08.08 delay 2 seconds @12:17:10.09 AS.reconfigure ASconfig @12:17:10.12 AS.seek 1.000ml @12:18:08.72 delay 1 second @12:18:09.73 AFV.select :holdingCoil @12:18:09.82 delay 1 second @12:18:10.83 AS.reconfigure ASfast @12:18:10.87 AS.seek 0.000ml @12:18:38.61 delay 3 seconds @12:18:41.62 AFV.select :carrierSolution @12:18:41.71 delay 2 seconds @12:18:43.72 AS.reconfigure ASconfig @12:18:43.76 AS.seek 1.000ml @12:19:42.39 delay 1 second @12:19:43.40 AFV.select :holdingCoil @12:19:43.49 delay 1 second @12:19:44.50 AS.reconfigure ASfast @12:19:44.53 AS.seek 0.000ml @12:20:12.23 delay 3 seconds @12:20:15.24 AFV.select :carrierSolution @12:20:15.33 delay 2 seconds @12:20:17.34 AS.reconfigure ASconfig @12:20:17.37 AS.seek 1.000ml @12:21:15.96 delay 1 second @12:21:16.97 AFV.select :holdingCoil @12:21:17.06 delay 1 second @12:21:18.07 AS.reconfigure ASnorm @12:21:18.10 AS.seek 0.000ml @12:22:09.57 Setting up PCR for ENTap -- writing /var/log/esp/ENTap14mar1912h1ml.pcr @12:22:09.58 Building PCR Slug from ASV with MM1 mix, PP1 primer @12:22:09.59 Priming with carrier @12:22:09.60 ATV.dial ARV @12:22:10.12 ARV.dial :carrier @12:22:10.42 AS.seek 0.500ml @12:22:36.35 AFV.select :carrierSolution @12:22:36.45 delay 2 seconds @12:22:38.46 AS.reconfigure ASconfig @12:22:38.51 AS.seek 1.000ml @12:23:07.96 delay 1 second @12:23:08.97 AFV.select :holdingCoil @12:23:09.06 delay 1 second @12:23:10.07 ATV.dial :waste @12:23:11.33 AS.reconfigure ASnorm @12:23:11.36 AS.seek empty @12:24:02.81 AFV.select :carrierSolution @12:24:02.91 delay 2 seconds @12:24:04.92 AS.reconfigure ASconfig @12:24:04.95 AS.seek 0.500ml @12:24:34.42 delay 1 second @12:24:35.43 AFV.select :holdingCoil @12:24:35.52 Building Slug @12:24:35.53 ATV.dial :ARV @12:24:36.78 delay 2 seconds @12:24:38.79 ARV.dial :air1 @12:24:39.11 delay 2 seconds @12:24:41.17 AS.reconfigure ASslow2 @12:24:41.20 AS.seek 0.506ml @12:24:47.64 delay 2 seconds @12:24:49.65 ARV.dial :MM1 @12:24:50.14 delay 2 seconds @12:24:52.15 Adding MM1 @12:24:52.21 AS.seek 0.524ml @12:25:10.94 delay 25 seconds @12:25:35.96 ARV.dial :PP1 @12:25:36.38 Adding PP1 @12:25:36.39 delay 2 seconds @12:25:38.44 AS.seek 0.530ml @12:25:44.74 delay 10 seconds @12:25:54.75 ARV.dial :air1 @12:25:55.06 adding ARV air @12:25:55.07 delay 2 seconds @12:25:57.13 AS.seek 0.536ml @12:26:03.40 delay 5 seconds @12:26:08.41 ARV.dial :carrier @12:26:08.73 delay 7 seconds @12:26:15.79 AS.seek 0.594ml @12:27:15.10 delay 5 seconds @12:27:20.11 ATV.dial :ASV @12:27:20.54 delay 2 seconds @12:27:22.60 AS.seek 0.600ml @12:27:29.05 delay 4 seconds @12:27:33.06 ATV.dial ARV @12:27:33.37 delay 2 seconds @12:27:35.37 ARV.dial :carrier @12:27:35.38 delay 2 seconds @12:27:37.43 AS.seek 0.650ml @12:28:28.64 delay 4 seconds @12:28:32.65 ATV.dial PCR @12:28:33.16 ASV.dial :waste1 @12:28:33.17 ARV.dial :air1 @12:28:33.51 Positioning Slug by 0.324ml @12:28:33.57 AS.seek 0.326ml @12:34:04.38 delay 5 seconds @12:34:09.39 ATV.dial ARV @12:34:10.01 ASV.dial :PCRlock @12:34:10.34 delay 5 seconds @12:34:15.35 Running ENTap Profile60C thermocycling @12:34:15.36 PCR.program Profile60C @12:34:15.46 Profile60C should complete in < 1:39:08 @12:34:15.47 PCR.run 2:08:55 @13:57:17.24 PCR.off @13:57:17.76 PCR for ENTap completed -- closed /var/log/esp/ENTap14mar1912h1ml.pcr @13:57:17.81 Cleaning PCR AFV.select :carrierSolution @13:57:17.91 delay 2 seconds @13:57:19.92 AS.reconfigure ASconfig @13:57:19.95 AS.seek 0.500ml @13:57:30.34 delay 1 second @13:57:31.35 AFV.select :holdingCoil @13:57:31.44 ASV.dial :waste1 @13:57:31.92 ATV.dial :air3 @13:57:32.93 AS.reconfigure ASslow1 @13:57:32.97 AS.seek 0.510ml @13:57:35.28 delay 2 seconds @13:57:37.29 ATV.dial :bleach @13:57:37.68 AS.seek 0.810ml @13:58:39.11 delay 3 seconds @13:58:42.12 ATV.dial :air3 @13:58:42.47 delay 3 seconds @13:58:45.53 AS.seek 0.835ml @13:58:50.87 delay 3 seconds @13:58:53.88 ATV.dial :bleach @13:58:54.36 AS.seek 0.820ml @13:58:57.68 delay 3 seconds @13:59:00.69 ARV.dial :waste @13:59:01.18 delay 3 seconds @13:59:04.19 ATV.dial :ARV @13:59:04.94 AS.reconfigure ASconfig @13:59:04.97 AS.seek 0.470ml @13:59:25.64 AFV.select :carrierSolution @13:59:25.73 delay 2 seconds @13:59:27.74 AS.seek 1.000ml @13:59:58.98 delay 1 second @13:59:59.99 AFV.select :holdingCoil @14:00:00.08 delay 1 second @14:00:01.09 AS.reconfigure ASfast @14:00:01.12 AS.seek 0.000ml @14:00:28.84 AFV.select :carrierSolution @14:00:28.94 delay 2 seconds @14:00:30.95 AS.reconfigure ASconfig @14:00:30.98 AS.seek 1.000ml @14:01:29.60 delay 1 second @14:01:30.61 AFV.select :holdingCoil @14:01:30.70 delay 1 second @14:01:31.71 AS.reconfigure ASfast @14:01:31.74 AS.seek 0.000ml @14:01:59.43 AFV.select :carrierSolution @14:01:59.53 delay 2 seconds @14:02:01.54 AS.reconfigure ASconfig @14:02:01.57 AS.seek 1.000ml @14:03:00.16 delay 1 second @14:03:01.17 AFV.select :holdingCoil @14:03:01.26 delay 1 second @14:03:02.27 AS.reconfigure ASfast @14:03:02.30 AS.seek 0.000ml @14:03:30.04 ARV.dial between carrier and air1 @14:03:30.46 ATV.dial :air3 @14:03:31.40 AS.reconfigure ASslow1 @14:03:31.44 AS.seek 0.010ml @14:03:33.75 delay 2 seconds @14:03:35.76 ATV.dial :bleach @14:03:36.15 AS.seek 0.810ml @14:06:19.58 delay 3 seconds @14:06:22.59 ATV.dial :air3 @14:06:22.94 delay 3 seconds @14:06:26.00 AS.seek 0.835ml @14:06:31.34 delay 3 seconds @14:06:34.35 ATV.dial :bleach @14:06:34.74 AS.seek 0.820ml @14:06:38.04 delay 3 seconds @14:06:41.05 ATV.dial PCR @14:06:41.37 AS.reconfigure ASconfig @14:06:41.40 AS.seek empty @14:07:29.54 PCR.program :bleachProfile @14:07:29.58 PCR program should complete in < 2:45 @14:07:29.59 PCR.run 10:00 @14:10:28.36 PCR.off @14:10:28.39 AFV.select :carrierSolution @14:10:28.48 delay 2 seconds @14:10:30.49 AS.seek 1.000ml @14:11:29.15 delay 1 second @14:11:30.16 AFV.select :holdingCoil @14:11:30.25 delay 1 second @14:11:31.26 AS.reconfigure ASfast @14:11:31.30 AS.seek 0.000ml @14:11:59.01 delay 3 seconds @14:12:02.02 AFV.select :carrierSolution @14:12:02.11 delay 2 seconds @14:12:04.12 AS.reconfigure ASconfig @14:12:04.15 AS.seek 1.000ml @14:13:02.75 delay 1 second @14:13:03.76 AFV.select :holdingCoil @14:13:03.95 delay 1 second @14:13:04.96 AS.reconfigure ASfast @14:13:04.99 AS.seek 0.000ml @14:13:32.73 delay 3 seconds @14:13:35.74 AFV.select :carrierSolution @14:13:35.83 delay 2 seconds @14:13:37.84 AS.reconfigure ASconfig @14:13:37.87 AS.seek 1.000ml @14:14:36.48 delay 1 second @14:14:37.49 AFV.select :holdingCoil @14:14:37.58 delay 1 second @14:14:38.59 AS.reconfigure ASfast @14:14:38.62 AS.seek 0.000ml @14:15:06.32 delay 3 seconds @14:15:09.33 AFV.select :carrierSolution @14:15:09.42 delay 2 seconds @14:15:11.43 AS.reconfigure ASconfig @14:15:11.46 AS.seek 1.000ml @14:16:10.08 delay 1 second @14:16:11.09 AFV.select :holdingCoil @14:16:11.18 delay 1 second @14:16:12.19 AS.reconfigure ASfast @14:16:12.22 AS.seek 0.000ml @14:16:39.96 delay 3 seconds @14:16:42.97 AFV.select :carrierSolution @14:16:43.06 delay 2 seconds @14:16:45.07 AS.reconfigure ASconfig @14:16:45.10 AS.seek 1.000ml @14:17:43.69 delay 1 second @14:17:44.70 AFV.select :holdingCoil @14:17:44.79 delay 1 second @14:17:45.80 AS.reconfigure ASnorm @14:17:45.83 AS.seek 0.000ml @14:18:37.28 Setting up PCR for ENTem -- writing /var/log/esp/ENTem14mar1914h1ml.pcr @14:18:37.29 Building PCR Slug from ASV with MM2 mix, PP3 primer @14:18:37.30 Priming with carrier @14:18:37.31 ATV.dial ARV @14:18:37.83 ARV.dial :carrier @14:18:38.11 AS.seek 0.500ml @14:19:03.98 AFV.select :carrierSolution @14:19:04.08 delay 2 seconds @14:19:06.09 AS.reconfigure ASconfig @14:19:06.13 AS.seek 1.000ml @14:19:35.58 delay 1 second @14:19:36.59 AFV.select :holdingCoil @14:19:36.68 delay 1 second @14:19:37.69 ATV.dial :waste @14:19:38.95 AS.reconfigure ASnorm @14:19:38.98 AS.seek empty @14:20:30.43 AFV.select :carrierSolution @14:20:30.53 delay 2 seconds @14:20:32.54 AS.reconfigure ASconfig @14:20:32.57 AS.seek 0.500ml @14:21:02.09 delay 1 second @14:21:03.10 AFV.select :holdingCoil @14:21:03.19 Building Slug @14:21:03.20 ATV.dial :ARV @14:21:04.45 delay 2 seconds @14:21:06.46 ARV.dial :air1 @14:21:06.79 delay 2 seconds @14:21:08.85 AS.reconfigure ASslow2 @14:21:08.88 AS.seek 0.506ml @14:21:15.13 delay 2 seconds @14:21:17.14 ARV.dial :MM2 @14:21:18.35 delay 2 seconds @14:21:20.36 Adding MM2 @14:21:20.42 AS.seek 0.524ml @14:21:38.96 delay 25 seconds @14:22:03.97 ARV.dial :PP3 @14:22:04.38 Adding PP3 @14:22:04.39 delay 2 seconds @14:22:06.44 AS.seek 0.530ml @14:22:12.73 delay 10 seconds @14:22:22.74 ARV.dial :air2 @14:22:23.08 adding ARV air @14:22:23.09 delay 2 seconds @14:22:25.14 AS.seek 0.536ml @14:22:31.49 delay 5 seconds @14:22:36.50 ARV.dial :carrier @14:22:37.52 delay 7 seconds @14:22:44.58 AS.seek 0.594ml @14:23:44.04 delay 5 seconds @14:23:49.05 ATV.dial :ASV @14:23:49.36 delay 2 seconds @14:23:51.42 AS.seek 0.600ml @14:23:57.90 delay 4 seconds @14:24:01.91 ATV.dial ARV @14:24:02.24 delay 2 seconds @14:24:04.25 ARV.dial :carrier @14:24:04.26 delay 2 seconds @14:24:06.31 AS.seek 0.650ml @14:24:57.54 delay 4 seconds @14:25:01.55 ATV.dial PCR @14:25:02.04 ASV.dial :waste1 @14:25:02.05 ARV.dial :air1 @14:25:02.40 Positioning Slug by 0.324ml @14:25:02.45 AS.seek 0.326ml @14:30:33.13 delay 5 seconds @14:30:38.14 ATV.dial ARV @14:30:38.66 ASV.dial :PCRlock @14:30:39.14 delay 5 seconds @14:30:44.15 Running ENTem ProfileEM60C thermocycling @14:30:44.16 PCR.program ProfileEM60C @14:30:44.27 ProfileEM60C should complete in < 2:11:38 @14:30:44.28 PCR.run 2:49:32.5 @16:27:11.33 PCR.off @16:27:11.87 PCR for ENTem completed -- closed /var/log/esp/ENTem14mar1914h1ml.pcr @16:27:11.92 Cleaning PCR AFV.select :carrierSolution @16:27:12.02 delay 2 seconds @16:27:14.03 AS.reconfigure ASconfig @16:27:14.12 AS.seek 0.500ml @16:27:24.54 delay 1 second @16:27:25.55 AFV.select :holdingCoil @16:27:25.64 ASV.dial :waste1 @16:27:26.12 ATV.dial :air3 @16:27:27.06 AS.reconfigure ASslow1 @16:27:27.09 AS.seek 0.510ml @16:27:29.39 delay 2 seconds @16:27:31.40 ATV.dial :bleach @16:27:31.78 AS.seek 0.810ml @16:28:33.21 delay 3 seconds @16:28:36.22 ATV.dial :air3 @16:28:36.63 delay 3 seconds @16:28:39.69 AS.seek 0.835ml @16:28:45.02 delay 3 seconds @16:28:48.03 ATV.dial :bleach @16:28:48.40 AS.seek 0.820ml @16:28:51.73 delay 3 seconds @16:28:54.74 ARV.dial :waste @16:28:55.24 delay 3 seconds @16:28:58.25 ATV.dial :ARV @16:28:59.06 AS.reconfigure ASconfig @16:28:59.10 AS.seek 0.470ml @16:29:19.77 AFV.select :carrierSolution @16:29:19.86 delay 2 seconds @16:29:21.87 AS.seek 1.000ml @16:29:53.05 delay 1 second @16:29:54.06 AFV.select :holdingCoil @16:29:54.15 delay 1 second @16:29:55.16 AS.reconfigure ASfast @16:29:55.19 AS.seek 0.000ml @16:30:22.93 AFV.select :carrierSolution @16:30:23.03 delay 2 seconds @16:30:25.04 AS.reconfigure ASconfig @16:30:25.07 AS.seek 1.000ml @16:31:23.69 delay 1 second @16:31:24.70 AFV.select :holdingCoil @16:31:24.79 delay 1 second @16:31:25.80 AS.reconfigure ASfast @16:31:25.83 AS.seek 0.000ml @16:31:53.58 AFV.select :carrierSolution @16:31:53.68 delay 2 seconds @16:31:55.69 AS.reconfigure ASconfig @16:31:55.72 AS.seek 1.000ml @16:32:54.33 delay 1 second @16:32:55.34 AFV.select :holdingCoil @16:32:55.43 delay 1 second @16:32:56.44 AS.reconfigure ASfast @16:32:56.47 AS.seek 0.000ml @16:33:24.22 ARV.dial between carrier and air1 @16:33:24.62 ATV.dial :air3 @16:33:25.54 AS.reconfigure ASslow1 @16:33:25.58 AS.seek 0.010ml @16:33:27.92 delay 2 seconds @16:33:29.93 ATV.dial :bleach @16:33:30.31 AS.seek 0.810ml @16:36:13.68 delay 3 seconds @16:36:16.69 ATV.dial :air3 @16:36:17.10 delay 3 seconds @16:36:20.73 AS.seek 0.835ml @16:36:26.07 delay 3 seconds @16:36:29.08 ATV.dial :bleach @16:36:29.46 AS.seek 0.820ml @16:36:32.76 delay 3 seconds @16:36:35.77 ATV.dial PCR @16:36:36.11 AS.reconfigure ASconfig @16:36:36.14 AS.seek empty @16:37:24.27 PCR.program :bleachProfile @16:37:24.33 PCR program should complete in < 2:45 @16:37:24.34 PCR.run 10:00 @16:40:22.53 PCR.off @16:40:22.56 AFV.select :carrierSolution @16:40:22.65 delay 2 seconds @16:40:24.66 AS.seek 1.000ml @16:41:23.32 delay 1 second @16:41:24.33 AFV.select :holdingCoil @16:41:24.42 delay 1 second @16:41:25.43 AS.reconfigure ASfast @16:41:25.46 AS.seek 0.000ml @16:41:53.19 delay 3 seconds @16:41:56.20 AFV.select :carrierSolution @16:41:56.29 delay 2 seconds @16:41:58.30 AS.reconfigure ASconfig @16:41:58.33 AS.seek 1.000ml @16:42:56.96 delay 1 second @16:42:57.97 AFV.select :holdingCoil @16:42:58.06 delay 1 second @16:42:59.07 AS.reconfigure ASfast @16:42:59.10 AS.seek 0.000ml @16:43:26.83 delay 3 seconds @16:43:29.84 AFV.select :carrierSolution @16:43:29.93 delay 2 seconds @16:43:31.94 AS.reconfigure ASconfig @16:43:31.97 AS.seek 1.000ml @16:44:30.56 delay 1 second @16:44:31.57 AFV.select :holdingCoil @16:44:31.66 delay 1 second @16:44:32.67 AS.reconfigure ASfast @16:44:32.70 AS.seek 0.000ml @16:45:00.44 delay 3 seconds @16:45:03.45 AFV.select :carrierSolution @16:45:03.54 delay 2 seconds @16:45:05.55 AS.reconfigure ASconfig @16:45:05.58 AS.seek 1.000ml @16:46:04.19 delay 1 second @16:46:05.20 AFV.select :holdingCoil @16:46:05.29 delay 1 second @16:46:06.30 AS.reconfigure ASfast @16:46:06.33 AS.seek 0.000ml @16:46:34.08 delay 3 seconds @16:46:37.09 AFV.select :carrierSolution @16:46:37.18 delay 2 seconds @16:46:39.19 AS.reconfigure ASconfig @16:46:39.22 AS.seek 1.000ml @16:47:37.81 delay 1 second @16:47:38.82 AFV.select :holdingCoil @16:47:38.91 delay 1 second @16:47:39.92 AS.reconfigure ASnorm @16:47:39.95 AS.seek 0.000ml @16:48:31.41 Setting up PCR for IPCap -- writing /var/log/esp/IPCap14mar1916h1ml.pcr @16:48:31.42 Building PCR Slug from ASV with MM1 mix, PP2 primer @16:48:31.43 Priming with carrier @16:48:31.44 ATV.dial ARV @16:48:32.03 ARV.dial :carrier @16:48:32.33 AS.seek 0.500ml @16:48:58.21 AFV.select :carrierSolution @16:48:58.31 delay 2 seconds @16:49:00.32 AS.reconfigure ASconfig @16:49:00.36 AS.seek 1.000ml @16:49:29.79 delay 1 second @16:49:30.80 AFV.select :holdingCoil @16:49:30.89 delay 1 second @16:49:31.90 ATV.dial :waste @16:49:33.16 AS.reconfigure ASnorm @16:49:33.19 AS.seek empty @16:50:24.68 AFV.select :carrierSolution @16:50:24.78 delay 2 seconds @16:50:26.79 AS.reconfigure ASconfig @16:50:26.82 AS.seek 0.500ml @16:50:56.29 delay 1 second @16:50:57.30 AFV.select :holdingCoil @16:50:57.39 Building Slug @16:50:57.40 ATV.dial :ARV @16:50:58.66 delay 2 seconds @16:51:00.67 ARV.dial :air1 @16:51:00.99 delay 2 seconds @16:51:03.05 AS.reconfigure ASslow2 @16:51:03.08 AS.seek 0.506ml @16:51:09.44 delay 2 seconds @16:51:11.45 ARV.dial :MM1 @16:51:12.04 delay 2 seconds @16:51:14.05 Adding MM1 @16:51:14.11 AS.seek 0.524ml @16:51:32.86 delay 25 seconds @16:51:57.87 ARV.dial :PP2 @16:51:58.28 Adding PP2 @16:51:58.29 delay 2 seconds @16:52:00.35 AS.seek 0.530ml @16:52:06.85 delay 10 seconds @16:52:16.86 ARV.dial :air2 @16:52:17.28 adding ARV air @16:52:17.29 delay 2 seconds @16:52:19.35 AS.seek 0.536ml @16:52:25.63 delay 5 seconds @16:52:30.64 ARV.dial :carrier @16:52:31.66 delay 7 seconds @16:52:38.72 AS.seek 0.594ml @16:53:37.95 delay 5 seconds @16:53:42.96 ATV.dial :ASV @16:53:43.26 delay 2 seconds @16:53:45.32 AS.seek 0.600ml @16:53:51.64 delay 4 seconds @16:53:55.65 ATV.dial ARV @16:53:55.98 delay 2 seconds @16:53:57.99 ARV.dial :carrier @16:53:58.00 delay 2 seconds @16:54:00.05 AS.seek 0.650ml @16:54:51.36 delay 4 seconds @16:54:55.37 ATV.dial PCR @16:54:55.87 ASV.dial :waste1 @16:54:55.88 ARV.dial :air1 @16:54:56.23 Positioning Slug by 0.324ml @16:54:56.82 AS.seek 0.326ml @17:00:27.46 delay 5 seconds @17:00:32.47 ATV.dial ARV @17:00:32.98 ASV.dial :PCRlock @17:00:33.46 delay 5 seconds @17:00:38.47 Running IPCap Profile59C thermocycling @17:00:38.48 PCR.program Profile59C @17:00:38.59 Profile59C should complete in < 1:39:08 @17:00:38.60 PCR.run 2:08:55 @18:25:12.37 PCR.off @18:25:12.91 PCR for IPCap completed -- closed /var/log/esp/IPCap14mar1916h1ml.pcr @18:25:12.95 Cleaning PCR @18:25:12.96 AFV.select :carrierSolution @18:25:13.05 delay 2 seconds @18:25:15.06 AS.reconfigure ASconfig @18:25:15.09 AS.seek 0.500ml @18:25:25.50 delay 1 second @18:25:26.51 AFV.select :holdingCoil @18:25:26.60 ASV.dial :waste1 @18:25:27.08 ATV.dial :air3 @18:25:28.02 AS.reconfigure ASslow1 @18:25:28.05 AS.seek 0.510ml @18:25:30.36 delay 2 seconds @18:25:32.37 ATV.dial :bleach @18:25:32.83 AS.seek 0.810ml @18:26:34.23 delay 3 seconds @18:26:37.24 ATV.dial :air3 @18:26:37.58 delay 3 seconds @18:26:40.64 AS.seek 0.835ml @18:26:46.00 delay 3 seconds @18:26:49.01 ATV.dial :bleach @18:26:49.50 AS.seek 0.820ml @18:26:52.81 delay 3 seconds @18:26:55.82 ARV.dial :waste @18:26:56.43 delay 3 seconds @18:26:59.44 ATV.dial :ARV @18:27:00.18 AS.reconfigure ASconfig @18:27:00.22 AS.seek 0.470ml @18:27:20.92 AFV.select :carrierSolution @18:27:21.01 delay 2 seconds @18:27:23.02 AS.seek 1.000ml @18:27:54.24 delay 1 second @18:27:55.25 AFV.select :holdingCoil @18:27:55.34 delay 1 second @18:27:56.35 AS.reconfigure ASfast @18:27:56.38 AS.seek 0.000ml @18:28:24.12 AFV.select :carrierSolution @18:28:24.21 delay 2 seconds @18:28:26.22 AS.reconfigure ASconfig @18:28:26.25 AS.seek 1.000ml @18:29:25.41 delay 1 second @18:29:26.42 AFV.select :holdingCoil @18:29:26.51 delay 1 second @18:29:27.52 AS.reconfigure ASfast @18:29:27.55 AS.seek 0.000ml @18:29:55.27 AFV.select :carrierSolution @18:29:55.37 delay 2 seconds @18:29:57.38 AS.reconfigure ASconfig @18:29:57.41 AS.seek 1.000ml @18:30:56.02 delay 1 second @18:30:57.03 AFV.select :holdingCoil @18:30:57.12 delay 1 second @18:30:58.13 AS.reconfigure ASfast @18:30:58.17 AS.seek 0.000ml @18:31:25.96 ARV.dial between carrier and air1 @18:31:26.47 ATV.dial :air3 @18:31:27.49 AS.reconfigure ASslow1 @18:31:27.52 AS.seek 0.010ml @18:31:29.83 delay 2 seconds @18:31:31.84 ATV.dial :bleach @18:31:32.32 AS.seek 0.810ml @18:34:15.70 delay 3 seconds @18:34:18.71 ATV.dial :air3 @18:34:19.18 delay 3 seconds @18:34:22.24 AS.seek 0.835ml @18:34:27.61 delay 3 seconds @18:34:30.62 ATV.dial :bleach @18:34:31.02 AS.seek 0.820ml @18:34:34.34 delay 3 seconds @18:34:37.35 ATV.dial PCR @18:34:37.68 AS.reconfigure ASconfig @18:34:37.71 AS.seek empty @18:35:25.81 PCR.program :bleachProfile @18:35:25.87 PCR program should complete in < 2:45 @18:35:25.88 PCR.run 10:00 @18:38:24.07 PCR.off @18:38:24.10 AFV.select :carrierSolution @18:38:24.19 delay 2 seconds @18:38:26.20 AS.seek 1.000ml @18:39:24.86 delay 1 second @18:39:25.87 AFV.select :holdingCoil @18:39:25.96 delay 1 second @18:39:26.97 AS.reconfigure ASfast @18:39:27.00 AS.seek 0.000ml @18:39:54.72 delay 3 seconds @18:39:57.73 AFV.select :carrierSolution @18:39:57.82 delay 2 seconds @18:39:59.83 AS.reconfigure ASconfig @18:39:59.86 AS.seek 1.000ml @18:40:58.48 delay 1 second @18:40:59.49 AFV.select :holdingCoil @18:40:59.58 delay 1 second @18:41:00.59 AS.reconfigure ASfast @18:41:00.62 AS.seek 0.000ml @18:41:28.35 delay 3 seconds @18:41:31.36 AFV.select :carrierSolution @18:41:31.45 delay 2 seconds @18:41:33.46 AS.reconfigure ASconfig @18:41:33.49 AS.seek 1.000ml @18:42:32.10 delay 1 second @18:42:33.11 AFV.select :holdingCoil @18:42:33.20 delay 1 second @18:42:34.21 AS.reconfigure ASfast @18:42:34.24 AS.seek 0.000ml @18:43:01.99 delay 3 seconds @18:43:05.00 AFV.select :carrierSolution @18:43:05.09 delay 2 seconds @18:43:07.10 AS.reconfigure ASconfig @18:43:07.13 AS.seek 1.000ml @18:44:05.75 delay 1 second @18:44:06.76 AFV.select :holdingCoil @18:44:06.85 delay 1 second @18:44:07.86 AS.reconfigure ASfast @18:44:07.89 AS.seek 0.000ml @18:44:35.63 delay 3 seconds @18:44:38.64 AFV.select :carrierSolution @18:44:38.73 delay 2 seconds @18:44:40.74 AS.reconfigure ASconfig @18:44:40.77 AS.seek 1.000ml @18:45:39.39 delay 1 second @18:45:40.40 AFV.select :holdingCoil @18:45:40.49 delay 1 second @18:45:41.50 AS.reconfigure ASnorm @18:45:41.53 AS.seek 0.000ml @18:46:33.00 Setting up PCR for IPCem -- writing /var/log/esp/IPCem14mar1918h1ml.pcr @18:46:33.01 Building PCR Slug from ASV with MM3 mix, PP5 primer @18:46:33.02 Priming with carrier @18:46:33.03 ATV.dial ARV @18:46:33.53 ARV.dial :carrier @18:46:33.80 AS.seek 0.500ml @18:46:59.67 AFV.select :carrierSolution @18:46:59.76 delay 2 seconds @18:47:01.77 AS.reconfigure ASconfig @18:47:01.80 AS.seek 1.000ml @18:47:31.23 delay 1 second @18:47:32.24 AFV.select :holdingCoil @18:47:32.33 delay 1 second @18:47:33.34 ATV.dial :waste @18:47:34.69 AS.reconfigure ASnorm @18:47:34.72 AS.seek empty @18:48:26.20 AFV.select :carrierSolution @18:48:26.30 delay 2 seconds @18:48:28.31 AS.reconfigure ASconfig @18:48:28.34 AS.seek 0.500ml @18:48:57.84 delay 1 second @18:48:58.85 AFV.select :holdingCoil @18:48:58.94 Building Slug @18:48:58.95 ATV.dial :ARV @18:49:00.18 delay 2 seconds @18:49:02.19 ARV.dial :air1 @18:49:02.52 delay 2 seconds @18:49:04.58 AS.reconfigure ASslow2 @18:49:04.61 AS.seek 0.506ml @18:49:10.99 delay 2 seconds @18:49:13.00 ARV.dial :MM3 @18:49:13.85 delay 2 seconds @18:49:15.86 Adding MM3 @18:49:15.91 AS.seek 0.524ml @18:49:34.59 delay 25 seconds @18:49:59.60 ARV.dial :PP5 @18:49:59.91 Adding PP5 @18:49:59.92 delay 2 seconds @18:50:01.98 AS.seek 0.530ml @18:50:08.21 delay 10 seconds @18:50:18.22 ARV.dial :air3 @18:50:18.65 adding ARV air @18:50:18.66 delay 2 seconds @18:50:21.23 AS.seek 0.536ml @18:50:27.54 delay 5 seconds @18:50:32.55 ARV.dial :carrier @18:50:33.58 delay 7 seconds @18:50:40.63 AS.seek 0.594ml @18:51:39.86 delay 5 seconds @18:51:44.87 ATV.dial :ASV @18:51:45.19 delay 2 seconds @18:51:47.25 AS.seek 0.600ml @18:51:53.72 delay 4 seconds @18:51:57.73 ATV.dial ARV @18:51:58.05 delay 2 seconds @18:52:00.06 ARV.dial :carrier @18:52:00.07 delay 2 seconds @18:52:02.12 AS.seek 0.650ml @18:52:53.36 delay 4 seconds @18:52:57.37 ATV.dial PCR @18:52:57.88 ASV.dial :waste1 @18:52:57.89 ARV.dial :air1 @18:52:58.23 Positioning Slug by 0.324ml @18:52:58.28 AS.seek 0.326ml @18:58:28.97 delay 5 seconds @18:58:33.98 ATV.dial ARV @18:58:34.50 ASV.dial :PCRlock @18:58:34.98 delay 5 seconds @18:58:39.99 Running IPCem ProfileEM59C thermocycling @18:58:40.00 PCR.program ProfileEM59C @18:58:40.11 ProfileEM59C should complete in < 2:11:38 @18:58:40.12 PCR.run 2:49:32.5 @20:21:33.16 Session Begun @20:21:40.53 -> puck.count @20:21:40.54 NameError in kevan -- undefined local variable or method `puck' for # @20:22:20.22 -> Puck.count @20:22:20.30 SE.seek down @20:22:20.59 SE.coast @20:22:20.68 SC.coast @20:22:20.72 SC.reconfigure SCtight @20:22:20.77 SC.seek 1 @20:22:24.65 SC.reconfigure SCloose @20:22:24.84 Elbow.reconfigure TightElbow @20:22:24.89 Elbow.seek 1 @20:22:26.76 Forearm.reconfigure TightForearm @20:22:26.81 Forearm.seek 1 + 800 counts @20:22:27.97 SE.reconfigure SEplace @20:22:28.04 SE.seek beamBreak @20:22:29.27 SE.configure SEconfig @20:22:29.95 SE.seek down @20:22:30.45 Forearm.reconfigure LooseForearm @20:22:30.50 Forearm.seek retract @20:22:30.56 SE.coast @20:22:31.44 SC.coast @20:22:31.49 SC.reconfigure SCtight @20:22:31.54 SC.seek 2 @20:22:32.18 Slide::Error in kevan -- Storage Carousel overCurrent @20:24:48.12 -> exit @20:24:48.13 Session Ended @20:25:13.80 Session Begun @20:25:21.97 -> showlog @20:25:34.67 -> showlog -100 @20:26:04.05 -> exit @20:26:04.06 Session Ended @20:55:37.92 PCR.off @20:55:38.46 PCR for IPCem completed -- closed /var/log/esp/IPCem14mar1918h1ml.pcr @20:55:38.47 Full MFB Decontamination @20:55:38.48 Decon extraction column and decon AIV @20:55:38.49 SPE.hold 2:46:40,55.0C @20:55:38.51 ASV.dial :SPEdelivery @20:55:39.24 delay 20 seconds @20:55:59.25 AFV.select :carrierSolution @20:55:59.35 delay 2 seconds @20:56:01.36 AS.reconfigure ASconfig @20:56:01.40 AS.seek 0.500ml @20:56:11.83 delay 1 second @20:56:12.84 AFV.select :holdingCoil @20:56:12.93 ATV.dial :air1 @20:56:14.44 AS.reconfigure ASslow1 @20:56:14.47 AS.seek 0.520ml @20:56:18.81 ATV.dial :ASV @20:56:20.70 ASV.dial :SPErecovery @20:56:21.25 AS.seek 0.620ml @20:56:41.92 ATV.dial :waste @20:56:43.38 AS.reconfigure ASnorm @20:56:43.41 AS.seek empty @20:57:15.48 AFV.select :holdingCoil @20:57:15.49 ATV.dial :waste @20:57:15.50 AS.reconfigure ASconfig @20:57:15.53 AS.seek 0.000ml @20:57:15.82 AFV.select :carrierSolution @20:57:15.93 delay 2 seconds @20:57:17.94 AS.seek 0.500ml @20:57:47.34 delay 1 second @20:57:48.35 AFV.select :holdingCoil @20:57:48.44 ATV.dial :air3 @20:57:49.16 AS.reconfigure ASslow1 @20:57:49.19 AS.seek 0.520ml @20:57:53.51 ATV.dial :air3 @20:57:53.57 AS.seek 0.530ml @20:57:55.85 delay 2 seconds @20:57:57.86 ATV.dial :bleach @20:57:58.35 AS.seek 0.710ml @20:58:35.30 delay 3 seconds @20:58:38.31 ATV.dial :air3 @20:58:38.63 delay 3 seconds @20:58:41.69 AS.seek 0.735ml @20:58:47.05 delay 3 seconds @20:58:50.06 ATV.dial :bleach @20:58:50.45 AS.seek 0.720ml @20:58:53.72 delay 3 seconds @20:58:56.73 ATV.dial ASV @20:58:57.60 ASV.dial :SPErecovery @20:58:57.66 AS.seek 0.365ml @21:00:10.30 ATV.dial :waste @21:00:11.76 AS.reconfigure ASnorm @21:00:11.79 AS.seek empty @21:00:30.83 ATV.dial :air3 @21:00:31.40 AS.reconfigure ASslow1 @21:00:31.43 AS.seek 0.009ml @21:00:33.69 delay 2 seconds @21:00:35.70 ATV.dial :bleach @21:00:36.18 AS.seek 0.579ml @21:02:32.69 delay 3 seconds @21:02:35.70 ATV.dial :air3 @21:02:36.03 delay 3 seconds @21:02:39.09 AS.seek 0.604ml @21:02:44.43 delay 3 seconds @21:02:47.44 ATV.dial :bleach @21:02:47.83 AS.seek 0.589ml @21:02:51.12 delay 3 seconds @21:02:54.13 ATV.dial :ASV @21:02:55.00 ASV.dial :wasteR @21:02:55.76 AS.seek 0.000ml @21:04:56.32 delay 1:00 @21:05:56.33 ATV.dial ASV @21:05:56.34 AFV.select :carrierSolution @21:05:56.43 delay 2 seconds @21:05:58.44 AS.reconfigure ASconfig @21:05:58.47 AS.seek 1.000ml @21:06:57.04 delay 1 second @21:06:58.05 AFV.select :holdingCoil @21:06:58.14 delay 1 second @21:06:59.15 ASV.dial :wasteR @21:06:59.16 AS.reconfigure ASslow1 @21:06:59.19 AS.seek 0.000ml @21:10:23.44 delay 10 seconds @21:10:33.45 ATV.dial :air3 @21:10:34.56 AS.seek 0.010ml @21:10:36.89 delay 2 seconds @21:10:38.90 ATV.dial :bleach @21:10:39.28 AS.seek 0.580ml @21:12:35.78 delay 3 seconds @21:12:38.79 ATV.dial :air3 @21:12:39.12 delay 3 seconds @21:12:42.18 AS.seek 0.605ml @21:12:47.54 delay 3 seconds @21:12:50.55 ATV.dial :bleach @21:12:51.04 AS.seek 0.590ml @21:12:54.37 delay 3 seconds @21:12:57.38 ATV.dial ASV @21:12:58.27 ASV.dial :SPEdelivery @21:12:59.03 AS.seek 0.000ml @21:14:59.59 delay 1:00 @21:15:59.60 AFV.select :carrierSolution @21:16:00.25 delay 2 seconds @21:16:02.26 AS.reconfigure ASconfig @21:16:02.30 AS.seek 1.000ml @21:17:00.90 delay 1 second @21:17:01.91 AFV.select :holdingCoil @21:17:02.01 delay 1 second @21:17:03.02 ATV.dial ASV @21:17:03.03 ASV.dial :SPEdelivery @21:17:03.04 AS.reconfigure ASslow1 @21:17:03.07 AS.seek 0.000ml @21:17:38.28 Session Begun @21:17:42.81 -> showlog 300 @21:20:27.44 delay 10 seconds @21:20:37.45 ATV.dial :air2 @21:20:38.19 AS.reconfigure ASslow0 @21:20:38.24 AS.seek 0.800ml @21:20:51.68 -> Clamp @21:22:00.14 delay 3 seconds @21:22:03.15 ATV.dial :ASV @21:22:03.88 ASV.dial :SPEdelivery @21:22:03.90 AS.reconfigure ASslow1 @21:22:03.96 AS.seek 0.030ml @21:22:44.14 -> SC @21:23:15.52 -> Thread["kevan"] @21:23:24.85 -> Thread["kevan"].lastErr @21:23:38.32 -> Thread["kevan"].lastErr.reply.status @21:24:41.28 delay 20 seconds @21:25:01.29 ATV.dial :waste,via => air3 @21:25:02.74 AS.reconfigure ASconfig @21:25:02.79 AS.seek empty @21:25:04.89 SPE.coast @21:25:04.99 Removing Bleach from Mixing Coil @21:25:05.00 AFV.select :carrierSolution @21:25:05.10 delay 2 seconds @21:25:07.11 AS.seek 0.500ml @21:25:07.14 -> SE @21:25:35.10 -> SE.down @21:25:35.12 SE.seek down @21:25:36.63 delay 1 second @21:25:37.64 AFV.select :holdingCoil @21:25:37.74 ATV.dial :ASV @21:25:39.26 ASV.dial :SPErecovery @21:25:39.81 AS.reconfigure ASslow1 @21:25:39.86 AS.seek 0.730ml @21:25:43.75 -> SE.home.down @21:25:43.76 SE.reconfigure SEconfig @21:25:43.82 SE.home.seek down @21:25:44.12 Slide::Error in brent -- Storage Elevator overCurrent @21:26:04.64 -> SC.jog 200 @21:26:04.65 SC.reconfigure SCloose @21:26:04.70 SC.jog 200 @21:26:12.54 -> SE.down @21:26:12.56 SE.reconfigure SEconfig @21:26:12.62 SE.seek down @21:26:24.22 -> SE.home.down @21:26:24.24 SE.reconfigure SEconfig @21:26:24.29 SE.home.seek down @21:26:24.59 Slide::Error in brent -- Storage Elevator overCurrent @21:26:27.04 ASV.dial :wasteD @21:26:27.57 AS.reconfigure ASnorm @21:26:27.61 AS.seek 0.000ml @21:26:40.95 -> SE.down @21:26:40.96 SE.reconfigure SEconfig @21:26:41.02 SE.seek down @21:27:05.21 AFV.select :carrierSolution @21:27:05.32 delay 2 seconds @21:27:07.33 AS.reconfigure ASconfig @21:27:07.38 AS.seek 0.500ml @21:27:36.82 delay 1 second @21:27:37.83 AFV.select :holdingCoil @21:27:37.93 ATV.dial ASV @21:27:37.94 ASV.dial :SPErecovery @21:27:38.93 AS.reconfigure ASslow0 @21:27:38.98 AS.seek 0.300ml @21:27:46.34 -> list Storage.method :ready! @21:27:59.68 ATV.dial :air2 @21:28:00.48 AS.reconfigure ASslow1 @21:28:00.53 AS.seek 0.320ml @21:28:04.91 ATV.dial ASV @21:28:05.79 AS.reconfigure ASslow0 @21:28:05.84 AS.seek 0.570ml @21:28:17.35 -> Storage.method :ready! @21:28:21.07 -> _.source @21:28:31.66 ASV.dial :wasteD @21:28:32.17 AS.reconfigure ASnorm @21:28:32.22 AS.seek 0.000ml @21:29:01.71 AFV.select :carrierSolution @21:29:01.82 delay 2 seconds @21:29:03.83 AS.reconfigure ASconfig @21:29:03.88 AS.seek 0.500ml @21:29:33.33 delay 1 second @21:29:34.34 AFV.select :holdingCoil @21:29:34.44 ATV.dial ASV @21:29:34.45 ASV.dial :SPErecovery @21:29:35.44 AS.reconfigure ASslow0 @21:29:35.49 AS.seek 0.300ml @21:29:56.19 ATV.dial :air2 @21:29:56.96 AS.reconfigure ASslow1 @21:29:57.01 AS.seek 0.320ml @21:30:01.38 ATV.dial ASV @21:30:02.27 AS.reconfigure ASslow0 @21:30:02.32 AS.seek 0.570ml @21:30:28.17 ASV.dial :wasteD @21:30:28.96 AS.reconfigure ASnorm @21:30:29.01 AS.seek 0.000ml @21:30:58.47 ATV.dial :air2 @21:30:59.29 AS.reconfigure ASslow0 @21:30:59.33 AS.seek 0.300ml @21:31:30.23 ATV.dial :ASV @21:31:31.04 ASV.dial :wasteD @21:31:31.06 AS.reconfigure ASconfig @21:31:31.11 AS.seek 0.050ml @21:31:45.98 ATV.dial :waste @21:31:47.53 AS.seek 0.000ml @21:31:50.74 ASV.dial :SPEdelivery @21:31:51.59 deconLysateINT @21:31:51.60 ATV.dial :air3 @21:31:52.59 -> Storage.coreReady! @21:31:52.60 SE.reconfigure SEconfig @21:31:52.68 SE.home.seek down @21:31:52.80 AS.reconfigure ASslow1 @21:31:52.85 AS.seek 0.010ml @21:31:53.02 Slide::Error in brent -- Storage Elevator overCurrent @21:31:53.04 SE.reconfigure SEconfig @21:31:53.09 SE.hold @21:31:53.17 SE.jog -128 @21:31:53.50 SC.reconfigure SCsloppy @21:31:53.55 SC.home.seek initial @21:31:55.21 delay 2 seconds @21:31:55.43 SE.reconfigure SEconfig @21:31:55.48 SE.home.seek down @21:31:57.22 ATV.dial :bleach @21:31:57.62 AS.seek 0.960ml @21:32:13.70 -> SC @21:32:16.86 -> SE @21:32:23.52 -> SE.home.down @21:32:23.53 SE.reconfigure SEconfig @21:32:23.59 SE.home.seek down @21:32:35.86 -> Puck.count @21:32:35.94 SE.seek down @21:32:36.01 SE.coast @21:32:36.09 SC.coast @21:32:36.13 ThreadError in brent -- kevan thread died holding Arm @21:32:36.14 SC.reconfigure SCtight @21:32:36.20 SC.seek 1 @21:32:38.42 SC.reconfigure SCloose @21:32:38.57 Elbow.seek 1 @21:32:38.73 Forearm.reconfigure TightForearm @21:32:38.79 Forearm.seek 1 + 800 counts @21:32:39.80 SE.reconfigure SEplace @21:32:39.87 SE.seek beamBreak @21:32:41.13 SE.configure SEconfig @21:32:41.23 SE.seek down @21:32:41.81 SE.coast @21:32:42.34 Forearm.reconfigure LooseForearm @21:32:42.39 Forearm.seek retract @21:32:43.35 SC.coast @21:32:43.40 SC.reconfigure SCtight @21:32:43.45 SC.seek 2 @21:32:45.64 SC.reconfigure SCloose @21:32:45.79 Elbow.seek 2 @21:32:46.10 Forearm.reconfigure TightForearm @21:32:46.15 Forearm.seek 2 + 800 counts @21:32:47.16 SE.reconfigure SEplace @21:32:47.23 SE.seek beamBreak @21:32:48.57 SE.configure SEconfig @21:32:48.68 SE.seek down @21:32:49.25 SE.coast @21:32:49.77 Forearm.reconfigure LooseForearm @21:32:49.82 Forearm.seek retract @21:32:50.77 SC.coast @21:32:50.82 SC.reconfigure SCtight @21:32:50.87 SC.seek 3 @21:32:53.20 SC.reconfigure SCloose @21:32:53.34 Elbow.seek 3 @21:32:53.57 Forearm.reconfigure TightForearm @21:32:53.62 Forearm.seek 3 + 800 counts @21:32:54.71 SE.reconfigure SEplace @21:32:54.78 SE.seek beamBreak @21:32:57.91 SE.configure SEconfig @21:32:58.01 SE.seek down @21:32:59.10 Forearm.reconfigure LooseForearm @21:32:59.15 Forearm.seek retract @21:33:00.64 SE.coast @21:33:00.68 SC.coast @21:33:00.73 SC.reconfigure SCtight @21:33:00.78 SC.seek 4 @21:33:02.95 SC.reconfigure SCloose @21:33:03.09 Elbow.seek 4 @21:33:03.25 Forearm.reconfigure TightForearm @21:33:03.30 Forearm.seek 4 + 800 counts @21:33:04.36 SE.reconfigure SEplace @21:33:04.43 SE.seek beamBreak @21:33:05.62 SE.configure SEconfig @21:33:05.72 SE.seek down @21:33:06.37 SE.coast @21:33:06.81 Forearm.reconfigure LooseForearm @21:33:06.86 Forearm.seek retract @21:33:07.81 SC.coast @21:33:07.86 SC.reconfigure SCtight @21:33:07.91 SC.seek 5 @21:33:10.14 SC.reconfigure SCloose @21:33:10.28 Elbow.seek 5 @21:33:10.58 Forearm.reconfigure TightForearm @21:33:10.63 Forearm.seek 5 + 800 counts @21:33:11.63 SE.reconfigure SEplace @21:33:12.31 SE.seek beamBreak @21:33:13.43 SE.configure SEconfig @21:33:13.53 SE.seek down @21:33:14.09 SE.coast @21:33:14.64 Forearm.reconfigure LooseForearm @21:33:14.69 Forearm.seek retract @21:33:15.67 SC.coast @21:33:15.72 SC.reconfigure SCtight @21:33:15.77 SC.seek 6 @21:33:18.01 SC.reconfigure SCloose @21:33:18.16 Elbow.seek 6 @21:33:18.47 Forearm.reconfigure TightForearm @21:33:18.52 Forearm.seek 6 + 800 counts @21:33:19.57 SE.reconfigure SEplace @21:33:19.64 SE.seek beamBreak @21:33:20.74 SE.configure SEconfig @21:33:20.84 SE.seek down @21:33:21.37 SE.coast @21:33:21.96 Forearm.reconfigure LooseForearm @21:33:22.01 Forearm.seek retract @21:33:22.95 SC.coast @21:33:23.00 SC.reconfigure SCtight @21:33:23.04 SC.seek 7 @21:33:25.26 SC.reconfigure SCloose @21:33:25.41 Elbow.seek 7 @21:33:25.69 Forearm.reconfigure TightForearm @21:33:25.74 Forearm.seek 7 + 800 counts @21:33:26.86 SE.reconfigure SEplace @21:33:26.93 SE.seek beamBreak @21:33:28.04 SE.configure SEconfig @21:33:28.14 SE.seek down @21:33:28.73 SE.coast @21:33:29.26 Forearm.reconfigure LooseForearm @21:33:29.31 Forearm.seek retract @21:33:30.26 SC.coast @21:34:18.98 -> SE.status @21:34:25.48 -> SE.down @21:34:25.50 SE.seek down @21:34:28.00 -> SE.status @21:34:44.44 -> SE.coast @21:34:44.45 SE.coast @21:34:49.82 -> SE.status @21:35:11.59 delay 3 seconds @21:35:14.60 ATV.dial :air3 @21:35:14.95 delay 3 seconds @21:35:18.01 AS.seek 0.985ml @21:35:23.38 delay 3 seconds @21:35:26.39 ATV.dial :bleach @21:35:26.80 AS.seek 0.970ml @21:35:30.11 delay 3 seconds @21:35:33.12 ATV.dial :AIV @21:35:34.31 AIV.dial :coilToMFB @21:35:34.32 AWV.select :waste @21:35:34.34 AFV.select :carrierSolution @21:35:34.45 delay 2 seconds @21:35:36.46 AS.reconfigure ASconfig @21:35:36.51 AS.seek 1.000ml @21:35:38.58 delay 1 second @21:35:39.59 AFV.select :holdingCoil @21:35:39.69 delay 1 second @21:35:40.70 AS.reconfigure ASslow0 @21:35:40.75 AS.seek empty @21:37:23.18 AFV.select :carrierSolution @21:37:23.29 delay 2 seconds @21:37:25.30 AS.reconfigure ASconfig @21:37:25.35 AS.seek 1.000ml @21:38:24.04 delay 1 second @21:38:25.05 AFV.select :holdingCoil @21:38:25.15 delay 1 second @21:38:26.16 AS.reconfigure ASslow0 @21:38:26.21 AS.seek empty @21:40:08.63 AFV.select :carrierSolution @21:40:08.74 delay 2 seconds @21:40:10.75 AS.reconfigure ASconfig @21:40:10.80 AS.seek 1.000ml @21:41:09.49 delay 1 second @21:41:10.50 AFV.select :holdingCoil @21:41:10.60 delay 1 second @21:41:11.61 AS.reconfigure ASslow0 @21:41:11.66 AS.seek empty @21:42:48.41 -> exit @21:42:48.43 Session Ended @21:42:54.08 ATV.dial :air2 @21:42:54.66 AS.reconfigure ASslow1 @21:42:54.69 AS.seek 0.009ml @21:42:57.00 AS.reconfigure ASslow0 @21:42:57.04 AS.seek 1.000ml @21:44:38.37 ATV.dial AIV @21:44:38.91 AS.seek 0.020ml @21:46:19.20 ATV.dial :waste @21:46:20.49 AS.seek 0.000ml @21:46:22.81 AWV.select :air @21:46:22.96 Cleaning PCR @21:46:22.97 AFV.select :carrierSolution @21:46:23.06 delay 2 seconds @21:46:25.07 AS.reconfigure ASconfig @21:46:25.10 AS.seek 0.500ml @21:46:54.52 delay 1 second @21:46:55.53 AFV.select :holdingCoil @21:46:55.62 ASV.dial :waste1 @21:46:55.95 ATV.dial :air3 @21:46:56.60 AS.reconfigure ASslow1 @21:46:56.64 AS.seek 0.510ml @21:46:58.95 delay 2 seconds @21:47:00.96 ATV.dial :bleach @21:47:01.36 AS.seek 0.810ml @21:47:33.00 Session Begun @21:47:58.31 -> Flush @21:48:02.79 delay 3 seconds @21:48:05.80 ATV.dial :air3 @21:48:06.15 delay 3 seconds @21:48:07.16 -> exit @21:48:07.17 Session Ended @21:48:09.22 AS.seek 0.835ml @21:48:14.55 delay 3 seconds @21:48:17.56 ATV.dial :bleach @21:48:18.05 AS.seek 0.820ml @21:48:21.39 delay 3 seconds @21:48:24.40 ARV.dial :waste @21:48:24.89 delay 3 seconds @21:48:27.90 ATV.dial :ARV @21:48:28.74 AS.reconfigure ASconfig @21:48:28.77 AS.seek 0.470ml @21:48:49.45 AFV.select :carrierSolution @21:48:49.55 delay 2 seconds @21:48:51.56 AS.seek 1.000ml @21:49:22.75 delay 1 second @21:49:23.76 AFV.select :holdingCoil @21:49:23.85 delay 1 second @21:49:24.86 AS.reconfigure ASfast @21:49:24.90 AS.seek 0.000ml @21:49:52.65 AFV.select :carrierSolution @21:49:52.75 delay 2 seconds @21:49:54.76 AS.reconfigure ASconfig @21:49:54.79 AS.seek 1.000ml @21:50:53.34 delay 1 second @21:50:54.35 AFV.select :holdingCoil @21:50:54.44 delay 1 second @21:50:55.45 AS.reconfigure ASfast @21:50:55.48 AS.seek 0.000ml @21:51:23.21 AFV.select :carrierSolution @21:51:23.31 delay 2 seconds @21:51:25.32 AS.reconfigure ASconfig @21:51:25.35 AS.seek 1.000ml @21:52:23.93 delay 1 second @21:52:24.94 AFV.select :holdingCoil @21:52:25.03 delay 1 second @21:52:26.04 AS.reconfigure ASfast @21:52:26.08 AS.seek 0.000ml @21:52:53.82 ARV.dial between carrier and air1 @21:52:54.23 ATV.dial :air3 @21:52:55.18 AS.reconfigure ASslow1 @21:52:55.21 AS.seek 0.010ml @21:52:57.57 delay 2 seconds @21:52:59.58 ATV.dial :bleach @21:52:59.98 AS.seek 0.810ml @21:55:43.44 delay 3 seconds @21:55:46.45 ATV.dial :air3 @21:55:46.80 delay 3 seconds @21:55:49.86 AS.seek 0.835ml @21:55:55.24 delay 3 seconds @21:55:58.25 ATV.dial :bleach @21:55:58.74 AS.seek 0.820ml @21:56:02.05 delay 3 seconds @21:56:05.06 ATV.dial PCR @21:56:05.51 AS.reconfigure ASconfig @21:56:05.54 AS.seek empty @21:56:53.68 PCR.program :bleachProfile @21:56:53.73 PCR program should complete in < 2:45 @21:56:53.74 PCR.run 10:00 @21:59:57.97 PCR.off @21:59:58.00 AFV.select :carrierSolution @21:59:58.09 delay 2 seconds @22:00:00.10 AS.seek 1.000ml @22:00:58.73 delay 1 second @22:00:59.74 AFV.select :holdingCoil @22:00:59.83 delay 1 second @22:01:00.84 AS.reconfigure ASfast @22:01:00.87 AS.seek 0.000ml @22:01:28.60 delay 3 seconds @22:01:31.61 AFV.select :carrierSolution @22:01:31.70 delay 2 seconds @22:01:33.71 AS.reconfigure ASconfig @22:01:33.74 AS.seek 1.000ml @22:02:32.33 delay 1 second @22:02:33.34 AFV.select :holdingCoil @22:02:33.43 delay 1 second @22:02:34.44 AS.reconfigure ASfast @22:02:34.47 AS.seek 0.000ml @22:03:02.21 delay 3 seconds @22:03:05.22 AFV.select :carrierSolution @22:03:05.31 delay 2 seconds @22:03:07.32 AS.reconfigure ASconfig @22:03:07.35 AS.seek 1.000ml @22:04:05.95 delay 1 second @22:04:06.96 AFV.select :holdingCoil @22:04:07.05 delay 1 second @22:04:08.06 AS.reconfigure ASfast @22:04:08.09 AS.seek 0.000ml @22:04:35.83 delay 3 seconds @22:04:38.84 AFV.select :carrierSolution @22:04:38.93 delay 2 seconds @22:04:40.94 AS.reconfigure ASconfig @22:04:40.97 AS.seek 1.000ml @22:05:39.56 delay 1 second @22:05:40.57 AFV.select :holdingCoil @22:05:40.66 delay 1 second @22:05:41.67 AS.reconfigure ASfast @22:05:41.70 AS.seek 0.000ml @22:06:09.40 delay 3 seconds @22:06:12.41 AFV.select :carrierSolution @22:06:12.50 delay 2 seconds @22:06:14.51 AS.reconfigure ASconfig @22:06:14.54 AS.seek 1.000ml @22:07:13.13 delay 1 second @22:07:14.14 AFV.select :holdingCoil @22:07:14.24 delay 1 second @22:07:15.25 AS.reconfigure ASnorm @22:07:15.28 AS.seek 0.000ml @22:08:06.73 AFV.select :carrierSolution @22:08:06.82 delay 2 seconds @22:08:08.83 AS.reconfigure ASconfig @22:08:08.87 AS.seek 1.000ml @22:09:07.42 delay 1 second @22:09:08.43 AFV.select :holdingCoil @22:09:08.52 delay 1 second @22:09:09.53 ATV.dial :waste @22:09:10.51 AS.reconfigure ASnorm @22:09:10.54 AS.seek 0.500ml @22:09:36.45 ATV.dial :waste2 @22:09:37.65 AS.seek 0.000ml @22:10:03.55
Cleaning PCR AFV.select :carrierSolution @22:10:03.65 delay 2 seconds @22:10:05.66 AS.reconfigure ASconfig @22:10:05.69 AS.seek 0.500ml @22:10:35.11 delay 1 second @22:10:36.13 AFV.select :holdingCoil @22:10:36.22 ASV.dial :waste1 @22:10:36.23 ATV.dial :air3 @22:10:37.72 AS.reconfigure ASslow1 @22:10:37.75 AS.seek 0.510ml @22:10:40.48 delay 2 seconds @22:10:42.49 ATV.dial :bleach @22:10:42.86 AS.seek 0.810ml @22:11:44.29 delay 3 seconds @22:11:47.30 ATV.dial :air3 @22:11:47.62 delay 3 seconds @22:11:50.68 AS.seek 0.835ml @22:11:56.07 delay 3 seconds @22:11:59.08 ATV.dial :bleach @22:11:59.56 AS.seek 0.820ml @22:12:02.82 delay 3 seconds @22:12:05.84 ARV.dial :waste @22:12:06.25 delay 3 seconds @22:12:09.26 ATV.dial :ARV @22:12:10.11 AS.reconfigure ASconfig @22:12:10.14 AS.seek 0.470ml @22:12:30.81 AFV.select :carrierSolution @22:12:30.90 delay 2 seconds @22:12:32.91 AS.seek 1.000ml @22:13:04.15 delay 1 second @22:13:05.16 AFV.select :holdingCoil @22:13:05.25 delay 1 second @22:13:06.26 AS.reconfigure ASfast @22:13:06.29 AS.seek 0.000ml @22:13:34.04 AFV.select :carrierSolution @22:13:34.13 delay 2 seconds @22:13:36.14 AS.reconfigure ASconfig @22:13:36.18 AS.seek 1.000ml @22:14:34.77 delay 1 second @22:14:35.78 AFV.select :holdingCoil @22:14:35.87 delay 1 second @22:14:36.88 AS.reconfigure ASfast @22:14:36.92 AS.seek 0.000ml @22:15:04.62 AFV.select :carrierSolution @22:15:04.71 delay 2 seconds @22:15:06.72 AS.reconfigure ASconfig @22:15:06.75 AS.seek 1.000ml @22:16:05.35 delay 1 second @22:16:06.36 AFV.select :holdingCoil @22:16:06.45 delay 1 second @22:16:07.46 AS.reconfigure ASfast @22:16:07.50 AS.seek 0.000ml @22:16:35.24 ARV.dial between carrier and air1 @22:16:35.75 ATV.dial :air3 @22:16:36.69 AS.reconfigure ASslow1 @22:16:36.72 AS.seek 0.010ml @22:16:39.11 delay 2 seconds @22:16:41.12 ATV.dial :bleach @22:16:41.51 AS.seek 0.810ml @22:19:24.89 delay 3 seconds @22:19:27.90 ATV.dial :air3 @22:19:28.25 delay 3 seconds @22:19:31.31 AS.seek 0.835ml @22:19:36.64 delay 3 seconds @22:19:39.66 ATV.dial :bleach @22:19:40.14 AS.seek 0.820ml @22:19:43.45 delay 3 seconds @22:19:46.46 ATV.dial PCR @22:19:46.91 AS.reconfigure ASconfig @22:19:46.94 AS.seek empty @22:20:35.06 PCR.program :bleachProfile @22:20:35.12 PCR program should complete in < 2:45 @22:20:35.13 PCR.run 10:00 @22:23:39.37 PCR.off @22:23:39.40 AFV.select :carrierSolution @22:23:39.49 delay 2 seconds @22:23:41.50 AS.seek 1.000ml @22:24:40.15 delay 1 second @22:24:41.16 AFV.select :holdingCoil @22:24:41.30 delay 1 second @22:24:42.31 AS.reconfigure ASfast @22:24:42.34 AS.seek 0.000ml @22:25:10.07 delay 3 seconds @22:25:13.08 AFV.select :carrierSolution @22:25:13.17 delay 2 seconds @22:25:15.18 AS.reconfigure ASconfig @22:25:15.21 AS.seek 1.000ml @22:26:13.84 delay 1 second @22:26:14.86 AFV.select :holdingCoil @22:26:14.95 delay 1 second @22:26:15.96 AS.reconfigure ASfast @22:26:15.99 AS.seek 0.000ml @22:26:43.73 delay 3 seconds @22:26:46.74 AFV.select :carrierSolution @22:26:46.83 delay 2 seconds @22:26:48.84 AS.reconfigure ASconfig @22:26:48.88 AS.seek 1.000ml @22:27:47.49 delay 1 second @22:27:48.50 AFV.select :holdingCoil @22:27:48.59 delay 1 second @22:27:49.60 AS.reconfigure ASfast @22:27:49.64 AS.seek 0.000ml @22:28:17.40 delay 3 seconds @22:28:20.41 AFV.select :carrierSolution @22:28:20.50 delay 2 seconds @22:28:22.51 AS.reconfigure ASconfig @22:28:22.54 AS.seek 1.000ml @22:29:21.13 delay 1 second @22:29:22.14 AFV.select :holdingCoil @22:29:22.23 delay 1 second @22:29:23.24 AS.reconfigure ASfast @22:29:23.27 AS.seek 0.000ml @22:29:51.00 delay 3 seconds @22:29:54.01 AFV.select :carrierSolution @22:29:54.10 delay 2 seconds @22:29:56.11 AS.reconfigure ASconfig @22:29:56.14 AS.seek 1.000ml @22:30:54.75 delay 1 second @22:30:55.77 AFV.select :holdingCoil @22:30:55.86 delay 1 second @22:30:56.87 AS.reconfigure ASnorm @22:30:56.91 AS.seek 0.000ml @22:31:48.36 Completed {wqPCR} begun 08:01:37 @22:31:48.39 Asleep til 08:01PDT20-Mar-14 @22:31:48.40 AS.stop @22:31:48.42
Sleeping until 08:01PDT20-Mar-14 @22:31:48.44 GoodNews.email "Then, in tube 4: wqPCR\n/home/esp/esp2/mission/14mar14sccwrp.rb:68",Subject => "Asleep until 08:01PDT20-Mar-14" @22:31:50.08 CC.stop @22:31:50.11 CS.stop @22:31:50.14 PC.stop @22:31:50.17 PS.stop @22:31:50.20 Forearm.stop @22:31:50.23 Elbow.stop @22:31:50.26 SS.stop @22:31:50.81 Sleepy.power :raw,:OFF @22:31:50.82 Sleepy.power :dwarf,:OFF Shutdown @22:41:08.17 Session Begun @22:41:11.20 -> showlog @22:41:28.82 -> showlog -100 @22:42:11.41 -> ESPmode=quick esp 14mar19sccwrp.rb @22:42:11.42 SyntaxError in kevan -- compile error kevan:3: syntax error, unexpected tIDENTIFIER, expecting $end ESPmode=quick esp 14mar19sccwrp.rb ^ @22:43:13.56 -> exit @22:43:13.57 Session Ended @23:29:25.45 Session Begun @23:29:49.02 -> abort! "start new WCR mission" @23:29:49.03 CmdServer::Client::Error in kevan -- Aborting Mission: start new WCR mission @23:29:49.06 BadNews.email "start new WCR mission",Subject => "14mar14sccwrp ABORTED!" @23:29:59.60 Session Ended @23:30:34.49PDT19-Mar-14 ESP2 v3.20 I2C Gateway v3.01 @23:30:34.83 Powering up Dwarves @23:30:41.73
Configuration for neo by maro@mbari.org /home/esp/esp2/shallow/neo/configure.rb @23:30:41.81 Configuration for MFB10 by preston@mbari.org /home/esp/esp2/mfb/MFB10/configure.rb @23:30:45.02 Sleepy.power :raw,:ON @23:30:45.72 SPE.coast @23:30:45.76 SPE.configure SPEconfig @23:30:45.79 AS.stop @23:30:45.82 AS.configure ASconfig @23:30:45.85 CC.stop @23:30:45.87 CC.configure CCconfig @23:30:45.90 CS.stop @23:30:45.92 CS.configure CSconfig @23:30:45.95 PC.stop @23:30:45.97 PC.configure PCconfig @23:30:46.00 PS.stop @23:30:46.02 PS.configure PSconfig @23:30:46.08 Forearm.stop @23:30:46.10 Forearm.configure LooseForearm @23:30:46.13 Elbow.stop @23:30:46.15 Elbow.configure LooseElbow @23:30:46.21 SC.stop @23:30:46.23 SC.configure SCloose @23:30:46.26 SE.stop @23:30:46.28 SE.configure SEconfig @23:30:46.31 SS.stop @23:30:46.33 SS.configure SSconfig @23:30:46.36 CH.coast @23:30:46.40 CH.configure CHconfig @23:30:46.43 PH.coast @23:30:46.47 PH.configure PHconfig @23:30:46.52 PCR.off @23:30:46.54 PCR.pidtemp= 50,0.1,0,300,25,25 @23:30:46.57 PCR.caltemp= 6.6661,0.0344,0.0 @23:30:46.60 All dwarves are running firmware version 3.74 @23:30:47.15 CTD.latitude = 36.8044 Degrees North in seawater @23:30:47.16 Initializing Sensors @23:30:47.20 GoodNews.email "Starting w/pucks in tube 4\n Ending 06:00PDT20-Mar-14",Subject => "14mar19sccwrp begun" @23:30:49.09
Asleep til +0:10 @23:30:49.13 Sleeping until 23:30 @23:30:49.15 Sleepy.power :raw,:OFF @23:30:49.16 Sleepy.power :dwarf,:OFF Shutdown @23:30:56.75 GoodNews.email "Then, in tube 4: archivestack\n/home/esp/esp2local/mission/14mar19sccwrp.rb:62",Subject => "Asleep until 23:30" @23:30:58.02
Powering up Dwarves @23:31:03.06 Sleepy.power :raw,:ON @23:31:03.53 SPE.configure SPEconfig @23:31:03.55 AS.configure ASconfig @23:31:03.58 CC.configure CCconfig @23:31:03.61 CS.configure CSconfig @23:31:03.66 PC.configure PCconfig @23:31:03.69 PS.configure PSconfig @23:31:03.72 Forearm.configure LooseForearm @23:31:03.76 Elbow.configure LooseElbow @23:31:03.79 SC.configure SCloose @23:31:03.82 SE.configure SEconfig @23:31:03.86 SS.configure SSconfig @23:31:03.89 CH.configure CHconfig @23:31:03.91 PH.configure PHconfig @23:31:03.94 Valve.reset @23:31:04.56 PCR.off @23:31:04.59 PCR.pidtemp= 50,0.1,0,300,25,25 @23:31:04.62 PCR.caltemp= 6.6661,0.0344,0.0 @23:31:04.73 Intake.select :close @23:31:04.74 Exhaust.select :close @23:31:04.75 CTV.dial :air @23:31:04.82 PSV.dial :air @23:31:04.85
PC.reconfigure PCconfig @23:31:04.89 PC.home.seek open @23:31:04.92 CSV.dial :waste1 @23:31:05.28 PS.reconfigure PSconfig @23:31:05.31 PS.home.seek 0.000ml @23:31:05.58 SSV.dial :spit,avoiding => exhaust @23:31:06.36 CS.reconfigure CSfast @23:31:06.39 SS.reconfigure SSconfig @23:31:06.48 SS.home.seek 0.00ml @23:31:06.50 CS.home.seek 0.000ml @23:31:06.58 CC.reconfigure CCconfig @23:31:06.61 CC.home.seek open @23:31:13.32 Instrument::ReadTimeout in pollContext -- Could not get prompt from CTD on ctd @23:31:13.34 BadNews.email "Could not get prompt from CTD on ctd",Subject => "Instrument::ReadTimeout in pollContext" @23:31:15.57 PS.coast @23:31:15.67
CC.seek open @23:31:15.69 PC.seek open @23:31:15.86
Forearm.reconfigure LooseForearm @23:31:15.90 Forearm.home.seek retract @23:31:16.65 Hand.grip @23:31:16.67
Elbow.jog -1500 @23:31:17.13 Elbow.reconfigure LooseElbow @23:31:17.17 Elbow.home.seek clear @23:31:20.69 SE.reconfigure SEconfig @23:31:20.72 SE.home.seek down @23:31:21.23 SC.reconfigure SCsloppy @23:31:21.27 SC.home.seek initial @23:31:33.36 Forearm.seek retract @23:31:33.42 Elbow.seek hammer @23:31:34.60 Forearm.reconfigure TightForearm @23:31:34.65 Forearm.seek 2 @23:31:35.65 Forearm.seek retract @23:31:36.76 Forearm.reconfigure LooseForearm @23:31:37.05 Starting initialPurge @23:31:37.06 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @23:31:37.11 Hand.release @23:31:37.99 Elbow.reconfigure TightElbow @23:31:38.02 Elbow.seek FlushPuck::Garage @23:31:39.78 Elbow.reconfigure LooseElbow @23:31:39.87 Forearm.seek garage @23:31:40.85 Hand.grip @23:31:41.61 Forearm.seek retract @23:31:42.63 Elbow.reconfigure TightElbow @23:31:42.67 Elbow.seek CC @23:31:45.45 Elbow.reconfigure LooseElbow @23:31:45.54 Forearm.reconfigure TightForearm @23:31:45.57 Forearm.seek CC @23:31:46.68 CC.seek closed @23:31:50.41 Collection Clamp onSpring/offSpring for 5/22 samples, on/off torque 1.98 @23:31:50.46 Hand.release @23:31:51.19 Forearm.reconfigure LooseForearm @23:31:51.22 Forearm.seek retract @23:31:52.18 Flushing the Collection Valve::Manifold @23:31:52.28 CS Pulling 5ml of flush CSV.dial CSR @23:31:53.52 CSR.select :flush @23:31:53.62 CS.reconfigure CSconfig @23:31:53.65 CS.seek 5.000ml @23:32:07.17 delay 5 seconds @23:32:12.18 CSR.select :air @23:32:12.31 CS.reconfigure CSfast @23:32:12.35 CS.seek 7.500ml @23:32:18.91 CSV.dial :waste @23:32:20.18 CS.reconfigure CSconfig @23:32:20.22 CS.seek 0.000ml @23:32:39.98 Purging external Intake @23:32:40.07 CSV.dial :flush @23:32:40.57 CS.reconfigure CSfast @23:32:40.61 CS.seek 10.000ml @23:33:04.29 delay 5 seconds @23:33:09.30 CSV.dial CBV @23:33:09.33
CBV.dial CSV @23:33:10.11 CTV.dial :intake @23:33:10.85 Intake.select :open @23:33:10.94 CS.seek 0.000ml @23:33:35.00 Intake.select :close @23:33:35.09 CTV.dial :air @23:33:35.82 Flushing upper manifold @23:33:35.91 CSV.dial :flush @23:33:36.70 CS.seek 5.000ml @23:33:48.94 delay 5 seconds @23:33:53.95 CSV.dial :air @23:33:54.41 CS.seek 7.000ml @23:33:59.85 CSV.dial CBV @23:33:59.87
CBV.dial CSV @23:34:00.94 CTV.dial :air @23:34:00.96 CS.seek 0.000ml @23:34:17.53 delay 7 seconds @23:34:24.54 CS.seek 3.000ml @23:34:32.19 CSV.dial :waste @23:34:32.70 CS.seek 0.000ml @23:34:40.58 CSV.dial :flush @23:34:41.01 CS.seek 3.000ml @23:34:48.69 delay 5 seconds @23:34:53.70 CSV.dial :air @23:34:54.18 CS.seek 5.000ml @23:34:59.59 CSV.dial :air @23:34:59.60 CS.seek 0.000ml @23:35:11.69 delay 7 seconds @23:35:18.70 CS.seek 3.000ml @23:35:26.31 CSV.dial :waste @23:35:27.10 CS.seek 0.000ml @23:35:34.78 Garaging FlushPuck in Collection Clamp @23:35:34.84 Elbow.reconfigure TightElbow @23:35:34.87 Elbow.seek CC @23:35:35.07 Elbow.reconfigure LooseElbow @23:35:35.15 Forearm.seek CC @23:35:36.11 CC.seek sealed @23:35:37.00 Hand.grip @23:35:37.77 CC.seek open @23:35:41.09 Forearm.seek retract @23:35:42.04 Elbow.reconfigure TightElbow @23:35:42.08 Elbow.seek garage @23:35:44.85 Elbow.reconfigure LooseElbow @23:35:44.94 Forearm.reconfigure TightForearm @23:35:44.97 Forearm.seek garage @23:35:46.15 Hand.release @23:35:46.93 Forearm.reconfigure LooseForearm @23:35:46.96 Forearm.seek retract @23:35:47.99 Starting stand-alone bac WCR @23:35:48.00 bac wcr sampling at most 1000ml @23:35:48.01 Whole Cell Archive sampling 1000ml @23:35:48.02 Loading wcr puck from tube 4 into Collection Clamp @23:35:48.04 BACfans.email "Can@23:31:37, 17.0C, 71% humidity, 14.3psia, 13.714V, 0.701A, 0.701A avg, 9.62W\nCTD data unavailable\nISUS data unavailable",Subject => "bac wcr sampling at most 1000ml" @23:35:49.85 SC.reconfigure SCtight @23:35:49.88 SC.seek 4 @23:35:53.81 SC.reconfigure SCloose @23:35:53.98 Elbow.reconfigure TightElbow @23:35:54.02 Elbow.seek 4 @23:35:55.86 Forearm.reconfigure TightForearm @23:35:55.90 Forearm.seek 4 + 800 counts @23:35:56.86 SE.reconfigure SEpick @23:35:56.91 SE.seek beamBreak @23:35:57.91 SE.configure SEconfig @23:35:58.03 Elbow.seek 4 @23:35:58.18 Forearm.seek 4 + 300 counts @23:35:58.41 Forearm.reconfigure LooseForearm @23:35:58.45 Hand.grip @23:35:59.16 SE.seek down @23:35:59.28 Forearm.seek retract @23:35:59.97 SE.coast @23:36:00.24 Elbow.seek CC @23:36:01.77 Elbow.reconfigure LooseElbow @23:36:01.86 Forearm.reconfigure TightForearm @23:36:01.89 Forearm.seek CC @23:36:03.01 CC.seek closed @23:36:06.75 Collection Clamp onSpring/offSpring for 5/22 samples, on/off torque 1.97 @23:36:06.76 Hand.release @23:36:07.56 Forearm.reconfigure LooseForearm @23:36:07.59 Forearm.seek retract @23:36:08.52 SC.coast @23:36:08.55 collectSample 1000ml w/bubble point of 55psi @23:36:08.57 Priming 7ml Sleepy.power :sampler,:ON @23:36:08.58 Intake.select :close Exhaust.select :close @23:36:08.59 SS.hold @23:36:08.74 Calibrating pressure and temperature sensors @23:36:08.76 CTV.dial :air @23:36:08.77 SSV.dial :spit,avoiding => exhaust @23:36:08.78 SS.reconfigure SSfast @23:36:08.81 SS.seek 5.00ml @23:36:11.67 SSV.dial CBV @23:36:11.69 CBV.dial SSV @23:36:13.02 delay 3.8 seconds @23:36:16.90 SSV.dial :spit @23:36:18.22 SS.seek 0.00ml @23:36:20.88 SSV.dial CBV @23:36:22.20 CTV.dial :intake @23:36:22.93 Waiting for external sampler to finish priming... @23:36:22.94 delayUntil "23:46:08.57" @23:46:08.57 SSV.dial CBV @23:46:08.59 CBV.dial SSV @23:46:08.60 Allowing 55s for 7.0ml of remaining 7.0ml Intake.select :open @23:46:10.73 Intake @14.5psia, vacuum from 8.0 to 12.0psi @23:46:10.74 SS.reconfigure forSampling @23:46:10.78 SS.seek 7.00ml @23:46:16.52 Stroke completed -- waiting for pressure to equalize ... @23:46:18.89 Intake.select :close @23:46:19.03 SSV.dial :exhaust @23:46:19.79 Exhaust.select :open @23:46:19.89 SS.reconfigure SSexhale @23:46:19.93 SS.seek 0.00ml @23:46:23.51 Exhaust.select :close @23:46:23.62 Sampling 1000.0ml @23:46:23.63 Intake.select :close @23:46:23.64 Exhaust.select :close @23:46:23.65 SS.reconfigure SSconfig @23:46:23.68 SS.hold @23:46:23.83 CTV.dial :intake @23:46:23.84 SSV.dial CBV @23:46:23.86 CBV.dial SSV @23:46:24.63 Allowing 185s for 25.0ml of remaining 1000.0ml Intake.select :open @23:46:26.78 Intake @14.0psia, vacuum from 7.5 to 11.5psi @23:46:26.79 SS.reconfigure forSampling @23:46:26.83 SS.seek 25.00ml @23:46:28.70 Vacuum too high -- waiting up to 40s @23:47:08.77 SS.reconfigure SSconfig @23:47:08.80 SS.hold @23:47:08.89 Intake.select :close @23:47:09.05 ShallowSampler::Clogged in WCR -- Intake @14.0psia, & Outlet @2.6psia -- no progress after 0.9ml @23:47:09.06 SSV.dial :exhaust @23:47:09.82 Exhaust.select :open @23:47:09.92 SS.reconfigure SSexhale @23:47:09.96 SS.seek 0.00ml @23:47:11.29 Exhaust.select :close @23:47:11.41 Sampled 0.9ml @23:47:11.42 collectSample: Complete @23:47:11.43 Drying puck before fixing for 04:00.00 CBV.dial :waste2b @23:47:12.18 delay 1:00 @23:47:14.40 Sleepy.power :sampler,:OFF @23:48:12.19 CSV.dial :air @23:48:12.99 CS.seek 10.000ml @23:48:36.63 CBV.dial between CSV and waste2b @23:48:36.95 Pumping wcr puck from tube 4 up to 55psi... @23:48:36.99 CBV.dial SSV @23:48:38.40 CBV.dial between CSV and waste2b @23:48:39.32 CSV.dial between air and CTV @23:48:40.84 CS.seek 7.348ml @23:48:47.80 CTV.dial CSV,avoiding => air @23:48:47.82 CSV.dial CTV @23:48:48.58 CS.reconfigure CSpressurize @23:48:48.61 CS.seek 0.000ml @23:49:07.75 CSV.dial :air @23:49:08.26 CS.reconfigure CSfast @23:49:08.29 CS.seek 10.000ml @23:49:31.76 CSV.dial between air and CTV @23:49:32.14 CS.seek 2.819ml @23:49:49.23 CTV.dial CSV,avoiding => air @23:49:49.24 CSV.dial CTV @23:49:49.53 CS.reconfigure CSpressurize @23:49:49.56 CS.seek 0.000ml @23:49:55.59 CBV.dial :waste2b @23:49:55.88 CS.reconfigure CSconfig @23:49:55.92 CS.seek empty @23:49:58.28 delay 1:00 @23:50:58.29 CTV.dial :air,avoiding => intake @23:50:59.56 Fix #1 of 2 (with rnal) for 02:00.00 @23:50:59.57 CS Pulling 2ml of rnal CSV.dial CSR @23:51:00.00 CSR.select :rnal @23:51:00.10 CS.seek 2.000ml @23:51:06.03 delay 5 seconds @23:51:11.04 CSR.select :air @23:51:11.13 CS.reconfigure CSfast @23:51:11.17 CS.seek 10.000ml @23:51:30.09 CBV.dial between CSV and waste2b @23:51:30.41 Pumping wcr puck from tube 4 up to 55psi... @23:51:30.45 CBV.dial SSV @23:51:31.86 CBV.dial between CSV and waste2b @23:51:32.80 CSV.dial between air and CTV @23:51:33.46 CS.seek 7.311ml @23:51:40.48 CTV.dial CSV,avoiding => air @23:51:40.50 CSV.dial CTV @23:51:41.85 CS.reconfigure CSpressurize @23:51:41.88 CS.seek 0.000ml @23:52:00.90 CSV.dial :air @23:52:01.33 CS.reconfigure CSfast @23:52:01.36 CS.seek 10.000ml @23:52:24.85 CSV.dial between air and CTV @23:52:26.05 CS.seek 2.155ml @23:52:44.60 CTV.dial CSV,avoiding => air @23:52:44.61 CSV.dial CTV @23:52:44.91 CS.reconfigure CSpressurize @23:52:44.94 CS.seek 0.000ml @23:52:46.33 delay 2:00 @23:54:46.34 CBV.dial :waste2b @23:54:46.64 CS.reconfigure CSconfig @23:54:46.68 CS.seek empty @23:54:51.99 delay 1:00 @23:55:52.00 CTV.dial :air,avoiding => intake @23:55:53.28 Fix #2 of 2 (with rnal) for 02:00.00 @23:55:53.29 CS Pulling 2ml of rnal CSV.dial CSR @23:55:53.72 CSR.select :rnal @23:55:53.83 CS.seek 2.000ml @23:55:59.67 delay 5 seconds @23:56:04.68 CSR.select :air @23:56:05.35 CS.reconfigure CSfast @23:56:05.38 CS.seek 10.000ml @23:56:24.33 CBV.dial between CSV and waste2b @23:56:24.67 Pumping wcr puck from tube 4 up to 55psi... @23:56:24.71 CBV.dial SSV @23:56:26.13 CBV.dial between CSV and waste2b @23:56:27.07 CSV.dial between air and CTV @23:56:27.73 CS.seek 7.175ml @23:56:34.96 CTV.dial CSV,avoiding => air @23:56:34.98 CSV.dial CTV @23:56:36.27 CS.reconfigure CSpressurize @23:56:36.30 CS.seek 0.000ml @23:56:55.01 CSV.dial :air @23:56:55.52 CS.reconfigure CSfast @23:56:55.55 CS.seek 10.000ml @23:57:18.99 CSV.dial between air and CTV @23:57:19.59 CS.seek 2.150ml @23:57:38.30 CTV.dial CSV,avoiding => air @23:57:38.31 CSV.dial CTV @23:57:38.69 CS.reconfigure CSpressurize @23:57:38.72 CS.seek 0.000ml @23:57:40.10 delay 2:00 @23:59:40.11 CBV.dial :waste2b @23:59:40.44 CS.reconfigure CSconfig @23:59:40.47 CS.seek empty @23:59:45.72 delay 1:00 @00:00:45.73PDT20-Mar-14 CTV.dial :air,avoiding => intake @00:00:47.04 Storing wcr puck in Collection Clamp to tube 3 @00:00:47.14 SE.seek down @00:00:47.40 SC.reconfigure SCtight @00:00:47.43 SC.seek 3 @00:00:49.69 SC.reconfigure SCloose @00:00:49.86 Elbow.reconfigure TightElbow @00:00:49.90 Elbow.seek 3 @00:00:51.51 Forearm.reconfigure TightForearm @00:00:51.54 Forearm.seek 3 + 800 counts @00:00:52.53 SE.reconfigure SEpick @00:00:52.58 SE.seek beamBreak @00:00:55.56 SE.configure SEconfig @00:00:55.67 Elbow.reconfigure LimpElbow @00:00:55.71 Forearm.reconfigure LooseForearm @00:00:55.75 Forearm.seek 3 @00:00:55.97 Elbow.hold @00:00:56.02 Elbow.reconfigure TightElbow @00:00:56.09 SE.seek 0.07 Pucks below Top Plate @00:00:56.42 Forearm.coast @00:00:56.45 Forearm.seek 3 + 334 counts @00:00:56.75 Forearm.reconfigure TightForearm @00:00:56.79 Forearm.seek 3 + 800 counts @00:00:57.04 SE.seek 1.07 Pucks below Top Plate @00:00:57.59 Forearm.reconfigure LooseForearm @00:00:57.62 Forearm.seek retract @00:00:58.63 Elbow.seek CC @00:01:00.16 Elbow.reconfigure LooseElbow @00:01:00.25 Forearm.seek CC @00:01:01.18 CC.seek sealed @00:01:02.10 Hand.grip @00:01:02.84 CC.seek open @00:01:06.23 Forearm.seek retract @00:01:07.20 Elbow.reconfigure TightElbow @00:01:07.24 Elbow.seek 3 @00:01:08.84 Elbow.reconfigure LimpElbow @00:01:08.89 Forearm.seek 3 @00:01:09.87 Elbow.hold @00:01:09.92 Elbow.reconfigure TightElbow @00:01:09.96 Hand.release @00:01:10.79 Forearm.coast @00:01:11.41 Forearm.seek 3 + 303 counts @00:01:11.68 Forearm.reconfigure TightForearm @00:01:11.72 Forearm.seek 3 + 800 counts @00:01:12.08 SE.seek down @00:01:13.19 Forearm.reconfigure LooseForearm @00:01:13.22 Forearm.seek retract @00:01:14.66 SE.coast @00:01:14.69 SC.coast @00:01:14.71 Starting finalPurge @00:01:14.72 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @00:01:14.87 Elbow.seek FlushPuck::Garage @00:01:16.54 Elbow.reconfigure LooseElbow @00:01:16.63 Forearm.seek garage @00:01:17.64 Hand.grip @00:01:18.42 Forearm.seek retract @00:01:19.40 Elbow.reconfigure TightElbow @00:01:19.44 Elbow.seek CC @00:01:22.19 Elbow.reconfigure LooseElbow @00:01:22.28 Forearm.reconfigure TightForearm @00:01:22.31 Forearm.seek CC @00:01:23.34 CC.seek closed @00:01:27.16 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.99 @00:01:27.21 Hand.release @00:01:28.00 Forearm.reconfigure LooseForearm @00:01:28.03 Forearm.seek retract @00:01:28.97 Flushing the Collection Valve::Manifold @00:01:29.07 CS Pulling 5ml of flush @00:01:29.08 CSV.dial CSR @00:01:29.50 CSR.select :flush @00:01:29.60 CS.seek 5.000ml @00:01:43.13 delay 5 seconds @00:01:48.14 CSR.select :air @00:01:48.28 CS.reconfigure CSfast @00:01:48.31 CS.seek 7.500ml @00:01:54.84 CSV.dial :waste @00:01:56.09 CS.reconfigure CSconfig @00:01:56.13 CS.seek 0.000ml @00:02:15.94 Flushing lower manifold's waste1 port @00:02:16.03 CSV.dial :flush @00:02:16.45 CS.reconfigure CSfast @00:02:16.49 CS.seek 5.000ml @00:02:28.69 delay 5 seconds @00:02:33.70 CSV.dial :air @00:02:34.17 CS.seek 7.001ml @00:02:39.50 CSV.dial CTV @00:02:39.52
CTV.dial CSV @00:02:40.81 CBV.dial :waste1 @00:02:41.55 CS.seek 0.000ml @00:02:57.94 Flushing lower manifold's waste2b port @00:02:58.03 CSV.dial :flush @00:02:58.82 CS.seek 5.000ml @00:03:10.97 delay 5 seconds @00:03:15.98 CSV.dial :air @00:03:16.57 CS.seek 7.000ml @00:03:21.93 CSV.dial CTV @00:03:21.95
CTV.dial CSV @00:03:22.39 CBV.dial :waste2b @00:03:23.13 CS.seek 0.000ml @00:03:39.59 Flushing lower manifold's waste1 port @00:03:39.60 CS Pulling 4ml of mfbkill @00:03:39.61 CSV.dial CSR @00:03:40.03 CSR.select :mfbkill @00:03:40.13 CS.reconfigure CSconfig @00:03:40.16 CS.seek 4.000ml @00:03:51.06 delay 5 seconds @00:03:56.07 CSR.select :air @00:03:56.16 CS.reconfigure CSfast @00:03:56.19 CS.seek 10.000ml @00:04:10.49 CSV.dial CTV @00:04:10.51
CTV.dial CSV @00:04:11.01 CBV.dial :waste1 @00:04:11.74 CS.seek 0.000ml @00:04:34.88 Flushing lower manifold's waste2b port @00:04:34.89 CS Pulling 4ml of mfbkill @00:04:34.90 CSV.dial CSR @00:04:35.39 CSR.select :mfbkill @00:04:35.49 CS.reconfigure CSconfig @00:04:35.52 CS.seek 4.000ml @00:04:46.44 delay 5 seconds @00:04:51.45 CSR.select :air @00:04:51.54 CS.reconfigure CSfast @00:04:51.57 CS.seek 10.000ml @00:05:05.94 CSV.dial CTV @00:05:05.96
CTV.dial CSV @00:05:06.47 CBV.dial :waste2b @00:05:07.21 CS.seek 0.000ml @00:05:30.38 Killing upper manifold @00:05:30.39 CS Pulling 4ml of mfbkill @00:05:30.40 CSV.dial CSR @00:05:30.89 CSR.select :mfbkill @00:05:30.99 CS.reconfigure CSconfig @00:05:31.02 CS.seek 4.000ml @00:05:41.91 delay 5 seconds @00:05:46.92 CSR.select :air @00:05:47.01 CS.reconfigure CSfast @00:05:47.67 CS.seek 10.000ml @00:06:01.97 CSV.dial CBV @00:06:01.99
CBV.dial CSV @00:06:03.01 CTV.dial :air @00:06:04.31 CS.seek 0.000ml @00:06:27.44 delay 7 seconds @00:06:34.46 CS.seek 3.000ml @00:06:42.03 CSV.dial :waste @00:06:42.46 CS.seek 0.000ml @00:06:49.97 Flushing upper manifold @00:06:50.07 CSV.dial :flush @00:06:50.49 CS.seek 5.000ml @00:07:02.62 delay 5 seconds @00:07:07.64 CSV.dial :air @00:07:08.11 CS.seek 7.000ml @00:07:13.50 CSV.dial CBV @00:07:13.52
CBV.dial CSV @00:07:14.59 CTV.dial :air @00:07:14.61 CS.seek 0.000ml @00:07:31.09 delay 7 seconds @00:07:38.11 CS.seek 3.000ml @00:07:45.71 CSV.dial :waste @00:07:46.22 CS.seek 0.000ml @00:07:51.70 Session Begun @00:07:53.86
CSV.dial :flush @00:07:54.32 CS.seek 3.000ml @00:07:59.51 -> showlog @00:08:01.99
delay 5 seconds @00:08:07.00 CSV.dial :air @00:08:07.48 CS.seek 5.000ml @00:08:12.84 CSV.dial :air @00:08:12.86 CS.seek 0.000ml @00:08:24.87 delay 7 seconds @00:08:31.88 CS.seek 3.000ml @00:08:39.46 CSV.dial :waste @00:08:40.27 CS.seek 0.000ml @00:08:47.99 Flushing lower manifold's waste1 port @00:08:48.10 CSV.dial :flush @00:08:48.63 CS.seek 5.000ml @00:09:00.71 delay 5 seconds @00:09:05.72 CSV.dial :air @00:09:06.20 CS.seek 7.000ml @00:09:11.55 CSV.dial CTV @00:09:11.57
CTV.dial CSV @00:09:12.91 CBV.dial :waste1 @00:09:13.38 CS.seek 0.000ml @00:09:29.83 Flushing lower manifold's waste2b port @00:09:29.94 CSV.dial :flush @00:09:30.73 CS.seek 5.000ml @00:09:42.80 delay 5 seconds @00:09:47.81 CSV.dial :air @00:09:48.37 CS.seek 7.000ml @00:09:53.77 CSV.dial CTV @00:09:53.79
CTV.dial CSV @00:09:54.24 CBV.dial :waste2b @00:09:55.00 CS.seek 0.000ml @00:10:11.49 Garaging FlushPuck in Collection Clamp @00:10:11.56 Elbow.reconfigure TightElbow @00:10:11.61 Elbow.seek CC @00:10:11.81 Elbow.reconfigure LooseElbow @00:10:11.91 Forearm.seek CC @00:10:12.89 CC.seek sealed @00:10:13.83 Hand.grip @00:10:14.57 CC.seek open @00:10:17.89 Forearm.seek retract @00:10:18.86 Elbow.reconfigure TightElbow @00:10:18.92 Elbow.seek garage @00:10:21.71 Elbow.reconfigure LooseElbow @00:10:21.81 Forearm.reconfigure TightForearm @00:10:21.85 Forearm.seek garage @00:10:23.03 Hand.release @00:10:23.86 Forearm.reconfigure LooseForearm @00:10:23.91 Forearm.seek retract @00:10:24.94 Starting stand-alone bac WCR @00:10:24.95 bac wcr sampling at most 1000ml @00:10:24.96 Whole Cell Archive sampling 1000ml @00:10:24.99 BACfans.email "Can@00:00:47, 17.2C, 70% humidity, 14.3psia, 13.699V, 0.799A, 0.799A avg, 10.95W\nCTD data unavailable\nISUS data unavailable",Subject => "bac wcr sampling at most 1000ml" @00:10:25.96 Loading wcr puck from tube 4 into Collection Clamp @00:10:26.14 SE.seek down @00:10:26.82 SC.reconfigure SCtight @00:10:26.86 SC.seek 4 @00:10:29.08 SC.reconfigure SCloose @00:10:29.28 Elbow.reconfigure TightElbow @00:10:29.33 Elbow.seek 4 @00:10:31.19 Forearm.reconfigure TightForearm @00:10:31.24 Forearm.seek 4 + 800 counts @00:10:32.24 SE.reconfigure SEpick @00:10:32.31 SE.seek beamBreak @00:10:33.44 SE.configure SEconfig @00:10:33.59 Elbow.seek 4 @00:10:33.74 Forearm.seek 4 + 300 counts @00:10:34.08 Forearm.reconfigure LooseForearm @00:10:34.13 Hand.grip @00:10:34.84 SE.seek down @00:10:34.98 Forearm.seek retract @00:10:35.72 SE.coast @00:10:35.98 Elbow.seek CC @00:10:37.50 Elbow.reconfigure LooseElbow @00:10:37.59 Forearm.reconfigure TightForearm @00:10:37.64 Forearm.seek CC @00:10:38.80 CC.seek closed @00:10:42.61 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 2.01 @00:10:42.62 Hand.release @00:10:43.36 Forearm.reconfigure LooseForearm @00:10:43.40 Forearm.seek retract @00:10:44.37 SC.coast @00:10:44.47 collectSample 1000ml w/bubble point of 55psi @00:10:44.48 Priming 7ml @00:10:44.52 Sleepy.power :sampler,:ON @00:10:44.58 Intake.select :close @00:10:44.59 Exhaust.select :close @00:10:44.75 SS.reconfigure SSconfig @00:10:44.87 SS.hold @00:10:45.26 Calibrating pressure sensors @00:10:45.27 CTV.dial :air @00:10:46.63 SSV.dial :spit,avoiding => exhaust @00:10:47.40 SS.reconfigure SSfast @00:10:47.44 SS.seek 5.00ml @00:10:50.22 SSV.dial CBV @00:10:50.25 CBV.dial SSV @00:10:51.59 delay 3.8 seconds @00:10:55.47 SSV.dial :spit @00:10:56.81 SS.seek 0.00ml @00:10:59.47 SSV.dial CBV @00:11:00.80 CTV.dial :intake @00:11:02.02 Waiting for external sampler to finish priming... @00:11:02.03 delayUntil "00:20:44.52" @00:20:44.52 SSV.dial CBV @00:20:44.54 CBV.dial SSV @00:20:44.56 Allowing 55s for 7.0ml of remaining 7.0ml @00:20:44.58 Intake.select :open @00:20:46.72 Intake @14.3psia, vacuum from 7.8 to 11.8psi @00:20:46.74 SS.reconfigure forSampling @00:20:46.80 SS.seek 7.00ml @00:20:52.05 Stroke completed -- waiting for pressure to equalize ... @00:20:52.69 Intake.select :close @00:20:52.83 SSV.dial :exhaust @00:20:53.59 Exhaust.select :open @00:20:53.69 SS.reconfigure SSexhale @00:20:53.74 SS.seek 0.00ml @00:20:57.75 Exhaust.select :close @00:20:57.86 Sampling 1000.0ml @00:20:57.88 Intake.select :close @00:20:57.89 Exhaust.select :close @00:20:57.90 SS.reconfigure SSconfig @00:20:57.96 SS.hold @00:20:58.12 CTV.dial :intake @00:20:58.13 SSV.dial CBV @00:20:58.16 CBV.dial SSV @00:20:58.93 Allowing 185s for 25.0ml of remaining 1000.0ml @00:20:58.94 Intake.select :open @00:21:01.08 Intake @14.0psia, vacuum from 7.5 to 11.5psi @00:21:01.10 SS.reconfigure forSampling @00:21:01.15 SS.seek 25.00ml @00:21:14.62 Stroke completed -- waiting for pressure to equalize ... @00:21:15.83 Intake.select :close @00:21:15.99 SSV.dial :exhaust @00:21:16.78 Exhaust.select :open @00:21:16.88 SS.reconfigure SSexhale @00:21:16.93 SS.seek 0.00ml @00:21:25.87 Exhaust.select :close @00:21:25.97 SSV.dial CBV @00:21:25.99 CBV.dial SSV @00:21:26.75 Allowing 185s for 25.0ml of remaining 975.0ml @00:21:26.77 Intake.select :open @00:21:28.91 Intake @14.3psia, vacuum from 7.8 to 11.8psi @00:21:28.93 SS.reconfigure forSampling @00:21:28.98 SS.seek 25.00ml @00:21:32.10 Vacuum too high -- waiting up to 40s @00:22:12.29 SS.reconfigure SSconfig @00:22:12.33 SS.hold @00:22:12.44 Intake.select :close @00:22:12.59 ShallowSampler::Clogged in WCR -- Intake @14.1psia, & Outlet @2.0psia -- no progress after 28.9ml @00:22:12.61 SSV.dial :exhaust @00:22:13.38 Exhaust.select :open @00:22:13.48 SS.reconfigure SSexhale @00:22:13.53 SS.seek 0.00ml @00:22:16.38 Exhaust.select :close @00:22:16.49 Sampled 28.9ml @00:22:16.50 collectSample: Complete @00:22:16.52 Drying puck before fixing for 04:00.00 @00:22:16.53 CBV.dial :waste2b @00:22:17.29 delay 1:00 @00:22:19.48 Sleepy.power :sampler,:OFF @00:23:17.29 CSV.dial :air @00:23:17.82 CS.seek 10.000ml @00:23:41.34 CBV.dial between CSV and waste2b @00:23:41.69 Pumping wcr puck from tube 4 up to 55psi... @00:23:41.75 CBV.dial SSV @00:23:43.17 CBV.dial between CSV and waste2b @00:23:44.12 CSV.dial between air and CTV @00:23:44.50 CS.seek 7.331ml @00:23:51.32 CTV.dial CSV,avoiding => air @00:23:51.34 CSV.dial CTV @00:23:52.11 CS.reconfigure CSpressurize @00:23:52.16 CS.seek 0.000ml @00:24:11.92 CSV.dial :air @00:24:12.45 CS.reconfigure CSfast @00:24:12.50 CS.seek 10.000ml @00:24:35.94 CSV.dial between air and CTV @00:24:36.55 CS.seek 2.823ml @00:24:53.47 CTV.dial CSV,avoiding => air @00:24:53.48 CSV.dial CTV @00:24:53.89 CS.reconfigure CSpressurize @00:24:53.94 CS.seek 0.000ml @00:24:59.17 CBV.dial :waste2b @00:24:59.50 CS.reconfigure CSconfig @00:24:59.54 CS.seek empty @00:25:02.71 delay 1:00 @00:26:02.72 CTV.dial :air,avoiding => intake @00:26:04.00 Fix #1 of 2 (with rnal) for 02:00.00 @00:26:04.01 CS Pulling 2ml of rnal @00:26:04.02 CSV.dial CSR @00:26:04.47 CSR.select :rnal @00:26:04.58 CS.seek 2.000ml @00:26:10.49 delay 5 seconds @00:26:15.50 CSR.select :air @00:26:15.61 CS.reconfigure CSfast @00:26:15.66 CS.seek 10.000ml @00:26:34.45 CBV.dial between CSV and waste2b @00:26:34.79 Pumping wcr puck from tube 4 up to 55psi... @00:26:34.85 CBV.dial SSV @00:26:36.28 CBV.dial between CSV and waste2b @00:26:37.22 CSV.dial between air and CTV @00:26:37.89 CS.seek 7.358ml @00:26:44.62 CTV.dial CSV,avoiding => air @00:26:44.64 CSV.dial CTV @00:26:45.93 CS.reconfigure CSpressurize @00:26:45.98 CS.seek 0.000ml @00:27:05.17 CSV.dial :air @00:27:05.70 CS.reconfigure CSfast @00:27:05.74 CS.seek 10.000ml @00:27:28.99 CSV.dial between air and CTV @00:27:29.37 CS.seek 2.115ml @00:27:47.87 CTV.dial CSV,avoiding => air @00:27:47.88 CSV.dial CTV @00:27:48.19 CS.reconfigure CSpressurize @00:27:48.24 CS.seek 0.000ml @00:27:49.13 delay 2:00 @00:29:49.14 CBV.dial :waste2b @00:29:49.47 CS.reconfigure CSconfig @00:29:49.51 CS.seek empty @00:29:55.20 delay 1:00 @00:30:55.21 CTV.dial :air,avoiding => intake @00:30:56.50 Fix #2 of 2 (with rnal) for 02:00.00 @00:30:56.51 CS Pulling 2ml of rnal @00:30:56.52 CSV.dial CSR @00:30:56.96 CSR.select :rnal @00:30:57.08 CS.seek 2.000ml @00:31:03.00 delay 5 seconds @00:31:08.02 CSR.select :air @00:31:08.13 CS.reconfigure CSfast @00:31:08.18 CS.seek 10.000ml @00:31:27.00 CBV.dial between CSV and waste2b @00:31:27.34 Pumping wcr puck from tube 4 up to 55psi... @00:31:27.40 CBV.dial SSV @00:31:28.83 CBV.dial between CSV and waste2b @00:31:29.77 CSV.dial between air and CTV @00:31:30.44 CS.seek 7.211ml @00:31:37.54 CTV.dial CSV,avoiding => air @00:31:37.56 CSV.dial CTV @00:31:38.90 CS.reconfigure CSpressurize @00:31:38.95 CS.seek 0.000ml @00:31:57.81 CSV.dial :air @00:31:58.26 CS.reconfigure CSfast @00:31:58.31 CS.seek 10.000ml @00:32:21.48 CSV.dial between air and CTV @00:32:22.58 CS.seek 2.108ml @00:32:41.17 CSV.dial CTV @00:32:41.19 CTV.dial CSV,avoiding => air @00:32:41.60 CS.reconfigure CSpressurize @00:32:41.65 CS.seek 0.000ml @00:32:42.74 delay 2:00 @00:34:42.75 CBV.dial :waste2b @00:34:43.08 CS.reconfigure CSconfig @00:34:43.12 CS.seek empty @00:34:48.59 delay 1:00 @00:35:48.60 CTV.dial :air,avoiding => intake @00:35:49.92 Storing wcr puck in Collection Clamp to tube 3 @00:35:50.03 SE.seek down @00:35:50.33 SC.reconfigure SCtight @00:35:50.38 SC.seek 3 @00:35:52.62 SC.reconfigure SCloose @00:35:52.80 Elbow.reconfigure TightElbow @00:35:52.85 Elbow.seek 3 @00:35:54.46 Forearm.reconfigure TightForearm @00:35:54.51 Forearm.seek 3 + 800 counts @00:35:55.60 SE.reconfigure SEpick @00:35:55.67 SE.seek beamBreak @00:35:58.63 SE.configure SEconfig @00:35:58.77 Elbow.reconfigure LimpElbow @00:35:58.81 Forearm.reconfigure LooseForearm @00:35:58.86 Forearm.seek 3 @00:35:59.10 Elbow.hold @00:35:59.17 Elbow.reconfigure TightElbow @00:35:59.25 SE.seek 1.06 Pucks below Top Plate @00:35:59.58 Forearm.coast @00:35:59.62 Forearm.seek 3 + 327 counts @00:35:59.92 Forearm.reconfigure TightForearm @00:35:59.97 Forearm.seek 3 + 800 counts @00:36:00.31 SE.seek 2.06 Pucks below Top Plate @00:36:00.85 Forearm.reconfigure LooseForearm @00:36:00.89 Forearm.seek retract @00:36:01.84 Elbow.seek CC @00:36:03.44 Elbow.reconfigure LooseElbow @00:36:03.54 Forearm.seek CC @00:36:04.50 CC.seek sealed @00:36:05.43 Hand.grip @00:36:06.14 CC.seek open @00:36:09.51 Forearm.seek retract @00:36:10.46 Elbow.reconfigure TightElbow @00:36:10.52 Elbow.seek 3 @00:36:12.16 Elbow.reconfigure LimpElbow @00:36:12.22 Forearm.seek 3 @00:36:13.24 Elbow.hold @00:36:13.32 Elbow.reconfigure TightElbow @00:36:13.37 Hand.release @00:36:14.21 Forearm.coast @00:36:14.27 Forearm.seek 3 + 325 counts @00:36:14.51 Forearm.reconfigure TightForearm @00:36:14.55 Forearm.seek 3 + 800 counts @00:36:14.87 SE.seek down @00:36:16.52 Forearm.reconfigure LooseForearm @00:36:16.57 Forearm.seek retract @00:36:17.37 SE.coast @00:36:17.49 SC.coast @00:36:17.53 Starting finalPurge @00:36:17.54 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @00:36:17.71 Elbow.seek FlushPuck::Garage @00:36:19.48 Elbow.reconfigure LooseElbow @00:36:19.58 Forearm.seek garage @00:36:20.64 Hand.grip @00:36:21.47 Forearm.seek retract @00:36:22.49 Elbow.reconfigure TightElbow @00:36:22.54 Elbow.seek CC @00:36:25.49 Elbow.reconfigure LooseElbow @00:36:25.59 Forearm.reconfigure TightForearm @00:36:25.63 Forearm.seek CC @00:36:26.76 CC.seek closed @00:36:30.54 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.99 @00:36:30.60 Hand.release @00:36:31.43 Forearm.reconfigure LooseForearm @00:36:31.47 Forearm.seek retract @00:36:32.41 Flushing the Collection Valve::Manifold @00:36:32.53 CS Pulling 5ml of flush CSV.dial CSR @00:36:32.97 CSR.select :flush @00:36:33.08 CS.seek 5.000ml @00:36:46.58 delay 5 seconds @00:36:51.59 CSR.select :air @00:36:51.75 CS.reconfigure CSfast @00:36:51.79 CS.seek 7.500ml @00:36:58.24 CSV.dial :waste @00:36:59.50 CS.reconfigure CSconfig @00:36:59.55 CS.seek 0.000ml @00:37:19.36 Flushing lower manifold's waste1 port @00:37:19.47 CSV.dial :flush @00:37:19.91 CS.reconfigure CSfast @00:37:19.96 CS.seek 5.000ml @00:37:32.02 delay 5 seconds @00:37:37.03 CSV.dial :air @00:37:37.74 CS.seek 7.000ml @00:37:43.17 CSV.dial CTV @00:37:43.19
CTV.dial CSV @00:37:44.52 CBV.dial :waste1 @00:37:45.26 CS.seek 0.000ml @00:38:01.61 Flushing lower manifold's waste2b port @00:38:01.72 CSV.dial :flush @00:38:02.53 CS.seek 5.000ml @00:38:14.52 delay 5 seconds @00:38:19.53 CSV.dial :air @00:38:20.10 CS.seek 7.000ml @00:38:25.52 CSV.dial CTV @00:38:25.54
CTV.dial CSV @00:38:25.99 CBV.dial :waste2b @00:38:26.75 CS.seek 0.000ml @00:38:43.21 Flushing lower manifold's waste1 port @00:38:43.22 CS Pulling 4ml of mfbkill @00:38:43.24 CSV.dial CSR @00:38:43.68 CSR.select :mfbkill @00:38:43.79 CS.reconfigure CSconfig @00:38:43.84 CS.seek 4.000ml @00:38:54.78 delay 5 seconds @00:38:59.79 CSR.select :air @00:38:59.90 CS.reconfigure CSfast @00:38:59.95 CS.seek 10.000ml @00:39:14.27 CSV.dial CTV @00:39:14.30
CTV.dial CSV @00:39:14.81 CBV.dial :waste1 @00:39:15.57 CS.seek 0.000ml @00:39:38.49 Flushing lower manifold's waste2b port @00:39:38.50 CS Pulling 4ml of mfbkill @00:39:38.52 CSV.dial CSR @00:39:44.30 CSR.select :mfbkill @00:39:44.41 CS.reconfigure CSconfig @00:39:44.45 CS.seek 4.000ml @00:39:55.37 delay 5 seconds @00:40:00.38 CSR.select :air @00:40:00.49 CS.reconfigure CSfast @00:40:00.54 CS.seek 10.000ml @00:40:15.26 CSV.dial CTV @00:40:15.28
CTV.dial CSV @00:40:15.79 CBV.dial :waste2b @00:40:16.53 CS.seek 0.000ml @00:40:39.49 Killing upper manifold @00:40:39.50 CS Pulling 4ml of mfbkill @00:40:39.51 CSV.dial CSR @00:40:40.04 CSR.select :mfbkill @00:40:40.15 CS.reconfigure CSconfig @00:40:40.19 CS.seek 4.000ml @00:40:51.21 delay 5 seconds @00:40:56.22 CSR.select :air @00:40:56.33 CS.reconfigure CSfast @00:40:56.38 CS.seek 10.000ml @00:41:10.58 CSV.dial CBV @00:41:10.60
CBV.dial CSV @00:41:11.63 CTV.dial :air @00:41:12.90 CS.seek 0.000ml @00:41:35.80 delay 7 seconds @00:41:42.81 CS.seek 3.000ml @00:41:50.36 CSV.dial :waste @00:41:50.81 CS.seek 0.000ml @00:41:58.39 Flushing upper manifold @00:41:58.49 CSV.dial :flush @00:41:58.93 CS.seek 5.000ml @00:42:10.92 delay 5 seconds @00:42:15.93 CSV.dial :air @00:42:16.41 CS.seek 7.001ml @00:42:21.83 CSV.dial CBV @00:42:21.85
CBV.dial CSV @00:42:22.95 CTV.dial :air @00:42:22.96 CS.seek 0.000ml @00:42:39.27 delay 7 seconds @00:42:46.28 CS.seek 3.000ml @00:42:53.83 CSV.dial :waste @00:42:54.35 CS.seek 0.000ml @00:43:01.94 CSV.dial :flush @00:43:02.38 CS.seek 3.000ml @00:43:09.89 delay 5 seconds @00:43:14.90 CSV.dial :air @00:43:15.38 CS.seek 5.000ml @00:43:20.73 CSV.dial :air @00:43:20.75 CS.seek 0.000ml @00:43:32.64 delay 7 seconds @00:43:39.65 CS.seek 3.000ml @00:43:47.20 CSV.dial :waste @00:43:48.01 CS.seek 0.000ml @00:43:55.61 Flushing lower manifold's waste1 port @00:43:55.72 CSV.dial :flush @00:43:56.24 CS.seek 5.000ml @00:44:08.20 delay 5 seconds @00:44:13.21 CSV.dial :air @00:44:13.70 CS.seek 7.000ml @00:44:19.10 CSV.dial CTV @00:44:19.12
CTV.dial CSV @00:44:20.45 CBV.dial :waste1 @00:44:20.91 CS.seek 0.000ml @00:44:37.23 Flushing lower manifold's waste2b port @00:44:37.34 CSV.dial :flush @00:44:38.14 CS.seek 5.000ml @00:44:50.10 delay 5 seconds @00:44:55.11 CSV.dial :air @00:44:55.67 CS.seek 7.000ml @00:45:00.95 CSV.dial CTV @00:45:00.97
CTV.dial CSV @00:45:01.42 CBV.dial :waste2b @00:45:02.18 CS.seek 0.000ml @00:45:18.57 Garaging FlushPuck in Collection Clamp @00:45:18.64 Elbow.reconfigure TightElbow @00:45:18.69 Elbow.seek CC @00:45:18.92 Elbow.reconfigure LooseElbow @00:45:19.02 Forearm.seek CC @00:45:19.98 CC.seek sealed @00:45:20.95 Hand.grip @00:45:21.75 CC.seek open @00:45:25.07 Forearm.seek retract @00:45:26.04 Elbow.reconfigure TightElbow @00:45:26.09 Elbow.seek garage @00:45:28.80 Elbow.reconfigure LooseElbow @00:45:28.90 Forearm.reconfigure TightForearm @00:45:28.94 Forearm.seek garage @00:45:30.10 Hand.release @00:45:31.39 Forearm.reconfigure LooseForearm @00:45:31.43 Forearm.seek retract @00:45:32.47 Starting stand-alone bac WCR @00:45:32.48 bac wcr sampling at most 1000ml @00:45:32.49 Whole Cell Archive sampling 1000ml @00:45:32.50 Loading wcr puck from tube 4 into Collection Clamp @00:45:32.53 BACfans.email "Can@00:40:47, 17.3C, 70% humidity, 14.3psia, 13.724V, 0.616A, 0.616A avg, 8.45W\nCTD data unavailable\nISUS data unavailable",Subject => "bac wcr sampling at most 1000ml" @00:45:33.74 SE.seek down @00:45:34.04 SC.reconfigure SCtight @00:45:34.09 SC.seek 4 @00:45:36.42 SC.reconfigure SCloose @00:45:36.61 Elbow.reconfigure TightElbow @00:45:36.66 Elbow.seek 4 @00:45:38.52 Forearm.reconfigure TightForearm @00:45:38.57 Forearm.seek 4 + 800 counts @00:45:39.63 SE.reconfigure SEpick @00:45:39.70 SE.seek beamBreak @00:45:40.96 SE.configure SEconfig @00:45:41.09 Elbow.seek 4 @00:45:41.25 Forearm.seek 4 + 300 counts @00:45:41.57 Forearm.reconfigure LooseForearm @00:45:41.63 Hand.grip @00:45:42.39 SE.seek down @00:45:42.53 Forearm.seek retract @00:45:43.37 SE.coast @00:45:43.50 Elbow.seek CC @00:45:45.01 Elbow.reconfigure LooseElbow @00:45:45.10 Forearm.reconfigure TightForearm @00:45:45.14 Forearm.seek CC @00:45:46.24 CC.seek closed @00:45:50.03 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 1.92 @00:45:50.04 Hand.release @00:45:50.79 Forearm.reconfigure LooseForearm @00:45:50.83 Forearm.seek retract @00:45:51.82 SC.coast @00:45:51.86 collectSample 1000ml w/bubble point of 55psi @00:45:51.88 Priming 7ml @00:45:51.89 Sleepy.power :sampler,:ON @00:45:51.91 Intake.select :close @00:45:51.92 Exhaust.select :close @00:45:51.93 SS.reconfigure SSconfig @00:45:51.99 SS.hold @00:45:52.15 Calibrating pressure sensors @00:45:52.16 CTV.dial :air @00:45:53.45 SSV.dial :spit,avoiding => exhaust @00:45:54.20 SS.reconfigure SSfast @00:45:54.25 SS.seek 5.00ml @00:45:56.96 SSV.dial CBV @00:45:57.45 CBV.dial SSV @00:45:58.78 delay 3.8 seconds @00:46:02.66 SSV.dial :spit @00:46:03.97 SS.seek 0.00ml @00:46:06.63 SSV.dial CBV @00:46:07.94 CTV.dial :intake @00:46:08.66 Waiting for external sampler to finish priming... @00:46:08.67 delayUntil "00:55:51.89" @00:55:51.90 SSV.dial CBV @00:55:51.92 CBV.dial SSV @00:55:51.94 Allowing 55s for 7.0ml of remaining 7.0ml @00:55:51.96 Intake.select :open @00:55:54.10 Intake @14.5psia, vacuum from 8.0 to 12.0psi @00:55:54.12 SS.reconfigure forSampling @00:55:54.20 SS.seek 7.00ml @00:55:59.70 Stroke completed -- waiting for pressure to equalize ... @00:56:00.34 Intake.select :close @00:56:00.48 SSV.dial :exhaust @00:56:01.25 Exhaust.select :open @00:56:01.35 SS.reconfigure SSexhale @00:56:01.40 SS.seek 0.00ml @00:56:05.30 Exhaust.select :close @00:56:05.41 Sampling 1000.0ml @00:56:05.43 Intake.select :close @00:56:05.44 Exhaust.select :close @00:56:05.45 SS.reconfigure SSconfig @00:56:05.50 SS.hold @00:56:05.66 CTV.dial :intake @00:56:05.67 SSV.dial CBV @00:56:05.70 CBV.dial SSV @00:56:06.48 Allowing 185s for 25.0ml of remaining 1000.0ml @00:56:06.49 Intake.select :open @00:56:08.63 Intake @14.4psia, vacuum from 7.9 to 11.9psi @00:56:08.65 SS.reconfigure forSampling @00:56:08.71 SS.seek 25.00ml @00:56:22.09 Stroke completed -- waiting for pressure to equalize ... @00:56:22.72 Intake.select :close @00:56:22.87 SSV.dial :exhaust @00:56:23.65 Exhaust.select :open @00:56:23.75 SS.reconfigure SSexhale @00:56:23.80 SS.seek 0.00ml @00:56:32.92 Exhaust.select :close @00:56:33.02 SSV.dial CBV @00:56:33.04 CBV.dial SSV @00:56:33.83 Allowing 185s for 25.0ml of remaining 975.0ml @00:56:33.85 Intake.select :open @00:56:35.99 Intake @14.4psia, vacuum from 7.9 to 11.9psi @00:56:36.01 SS.reconfigure forSampling @00:56:36.07 SS.seek 25.00ml @00:56:49.50 Stroke completed -- waiting for pressure to equalize ... @00:56:50.13 Intake.select :close @00:56:50.28 SSV.dial :exhaust @00:56:51.04 Exhaust.select :open @00:56:51.14 SS.reconfigure SSexhale @00:56:51.19 SS.seek 0.00ml @00:56:59.92 Exhaust.select :close @00:57:00.02 SSV.dial CBV @00:57:00.04 CBV.dial SSV @00:57:00.82 Allowing 185s for 25.0ml of remaining 950.0ml @00:57:00.83 Intake.select :open @00:57:02.98 Intake @14.3psia, vacuum from 7.8 to 11.8psi @00:57:03.00 SS.reconfigure forSampling @00:57:03.06 SS.seek 25.00ml @00:57:16.81 Stroke completed -- waiting for pressure to equalize ... @00:57:17.44 Intake.select :close @00:57:17.59 SSV.dial :exhaust @00:57:18.37 Exhaust.select :open @00:57:18.47 SS.reconfigure SSexhale @00:57:18.52 SS.seek 0.00ml @00:57:27.39 Exhaust.select :close @00:57:27.49 SSV.dial CBV @00:57:27.51 CBV.dial SSV @00:57:28.28 Allowing 185s for 25.0ml of remaining 925.0ml @00:57:28.30 Intake.select :open @00:57:30.45 Intake @14.3psia, vacuum from 7.8 to 11.8psi @00:57:30.47 SS.reconfigure forSampling @00:57:30.53 SS.seek 25.00ml @00:57:44.37 Stroke completed -- waiting for pressure to equalize ... @00:57:45.00 Intake.select :close @00:57:45.14 SSV.dial :exhaust @00:57:45.90 Exhaust.select :open @00:57:46.01 SS.reconfigure SSexhale @00:57:46.06 SS.seek 0.00ml @00:57:54.95 Exhaust.select :close @00:57:55.05 SSV.dial CBV @00:57:55.07 CBV.dial SSV @00:57:55.85 Allowing 185s for 25.0ml of remaining 900.0ml @00:57:55.87 Intake.select :open @00:57:58.01 Intake @14.4psia, vacuum from 7.9 to 11.9psi @00:57:58.03 SS.reconfigure forSampling @00:57:58.08 SS.seek 25.00ml @00:58:12.37 Stroke completed -- waiting for pressure to equalize ... @00:58:13.01 Intake.select :close @00:58:13.15 SSV.dial :exhaust @00:58:13.93 Exhaust.select :open @00:58:14.03 SS.reconfigure SSexhale @00:58:14.08 SS.seek 0.00ml @00:58:23.07 Exhaust.select :close @00:58:23.17 SSV.dial CBV @00:58:23.19 CBV.dial SSV @00:58:23.98 Allowing 185s for 25.0ml of remaining 875.0ml @00:58:24.00 Intake.select :open @00:58:26.15 Intake @14.5psia, vacuum from 8.0 to 12.0psi @00:58:26.17 SS.reconfigure forSampling @00:58:26.23 SS.seek 25.00ml @00:58:40.10 Stroke completed -- waiting for pressure to equalize ... @00:58:40.74 Intake.select :close @00:58:40.88 SSV.dial :exhaust @00:58:41.66 Exhaust.select :open @00:58:41.76 SS.reconfigure SSexhale @00:58:41.81 SS.seek 0.00ml @00:58:50.60 Exhaust.select :close @00:58:50.70 SSV.dial CBV @00:58:50.72 CBV.dial SSV @00:58:51.51 Allowing 185s for 25.0ml of remaining 850.0ml @00:58:51.53 Intake.select :open @00:58:53.67 Intake @14.3psia, vacuum from 7.8 to 11.8psi @00:58:53.69 SS.reconfigure forSampling @00:58:53.75 SS.seek 25.00ml @00:59:07.87 Stroke completed -- waiting for pressure to equalize ... @00:59:08.50 Intake.select :close @00:59:08.64 SSV.dial :exhaust @00:59:09.41 Exhaust.select :open @00:59:09.51 SS.reconfigure SSexhale @00:59:09.56 SS.seek 0.00ml @00:59:18.26 Exhaust.select :close @00:59:18.36 SSV.dial CBV @00:59:18.38 CBV.dial SSV @00:59:19.15 Allowing 185s for 25.0ml of remaining 825.0ml @00:59:19.17 Intake.select :open @00:59:21.32 Intake @14.4psia, vacuum from 7.9 to 11.9psi @00:59:21.34 SS.reconfigure forSampling @00:59:21.39 SS.seek 25.00ml @00:59:35.54 Stroke completed -- waiting for pressure to equalize ... @00:59:36.18 Intake.select :close @00:59:36.31 SSV.dial :exhaust @00:59:37.08 Exhaust.select :open @00:59:37.18 SS.reconfigure SSexhale @00:59:37.23 SS.seek 0.00ml @00:59:46.00 Exhaust.select :close @00:59:46.10 SSV.dial CBV @00:59:46.12 CBV.dial SSV @00:59:46.88 Allowing 185s for 25.0ml of remaining 800.0ml @00:59:46.90 Intake.select :open @00:59:49.05 Intake @14.4psia, vacuum from 7.9 to 11.9psi @00:59:49.07 SS.reconfigure forSampling @00:59:49.13 SS.seek 25.00ml @01:00:02.94 Stroke completed -- waiting for pressure to equalize ... @01:00:03.57 Intake.select :close @01:00:03.71 SSV.dial :exhaust @01:00:04.49 Exhaust.select :open @01:00:04.59 SS.reconfigure SSexhale @01:00:04.64 SS.seek 0.00ml @01:00:13.41 Exhaust.select :close @01:00:13.52 SSV.dial CBV @01:00:13.54 CBV.dial SSV @01:00:14.30 Allowing 185s for 25.0ml of remaining 775.0ml @01:00:14.32 Intake.select :open @01:00:16.46 Intake @14.4psia, vacuum from 7.9 to 11.9psi @01:00:16.48 SS.reconfigure forSampling @01:00:16.53 SS.seek 25.00ml @01:00:30.39 Stroke completed -- waiting for pressure to equalize ... @01:00:31.02 Intake.select :close @01:00:31.16 SSV.dial :exhaust @01:00:31.94 Exhaust.select :open @01:00:32.04 SS.reconfigure SSexhale @01:00:32.09 SS.seek 0.00ml @01:00:41.05 Exhaust.select :close @01:00:41.15 SSV.dial CBV @01:00:41.17 CBV.dial SSV @01:00:41.95 Allowing 185s for 25.0ml of remaining 750.0ml @01:00:41.97 Intake.select :open @01:00:44.11 Intake @14.4psia, vacuum from 7.9 to 11.9psi @01:00:44.13 SS.reconfigure forSampling @01:00:44.18 SS.seek 25.00ml @01:00:58.02 Stroke completed -- waiting for pressure to equalize ... @01:00:58.65 Intake.select :close @01:00:58.79 SSV.dial :exhaust @01:00:59.58 Exhaust.select :open @01:00:59.68 SS.reconfigure SSexhale @01:00:59.73 SS.seek 0.00ml @01:01:08.97 Exhaust.select :close @01:01:09.07 SSV.dial CBV @01:01:09.09 CBV.dial SSV @01:01:09.86 Allowing 185s for 25.0ml of remaining 725.0ml @01:01:09.88 Intake.select :open @01:01:12.03 Intake @14.3psia, vacuum from 7.8 to 11.8psi @01:01:12.05 SS.reconfigure forSampling @01:01:12.10 SS.seek 25.00ml @01:01:25.80 Stroke completed -- waiting for pressure to equalize ... @01:01:26.44 Intake.select :close @01:01:26.58 SSV.dial :exhaust @01:01:27.34 Exhaust.select :open @01:01:27.44 SS.reconfigure SSexhale @01:01:27.49 SS.seek 0.00ml @01:01:36.39 Exhaust.select :close @01:01:36.49 SSV.dial CBV @01:01:36.51 CBV.dial SSV @01:01:37.28 Allowing 185s for 25.0ml of remaining 700.0ml @01:01:37.30 Intake.select :open @01:01:39.46 Intake @14.3psia, vacuum from 7.8 to 11.8psi @01:01:39.47 SS.reconfigure forSampling @01:01:39.53 SS.seek 25.00ml @01:01:53.72 Stroke completed -- waiting for pressure to equalize ... @01:01:54.36 Intake.select :close @01:01:54.50 SSV.dial :exhaust @01:01:55.28 Exhaust.select :open @01:01:55.38 SS.reconfigure SSexhale @01:01:55.43 SS.seek 0.00ml @01:02:04.25 Exhaust.select :close @01:02:04.36 SSV.dial CBV @01:02:04.38 CBV.dial SSV @01:02:05.15 Allowing 185s for 25.0ml of remaining 675.0ml @01:02:05.17 Intake.select :open @01:02:07.32 Intake @14.5psia, vacuum from 8.0 to 12.0psi @01:02:07.34 SS.reconfigure forSampling @01:02:07.40 SS.seek 25.00ml @01:02:21.18 Stroke completed -- waiting for pressure to equalize ... @01:02:21.81 Intake.select :close @01:02:21.95 SSV.dial :exhaust @01:02:22.73 Exhaust.select :open @01:02:22.83 SS.reconfigure SSexhale @01:02:22.88 SS.seek 0.00ml @01:02:31.76 Exhaust.select :close @01:02:31.86 SSV.dial CBV @01:02:31.88 CBV.dial SSV @01:02:32.67 Allowing 185s for 25.0ml of remaining 650.0ml @01:02:32.69 Intake.select :open @01:02:34.84 Intake @14.5psia, vacuum from 8.0 to 12.0psi @01:02:34.86 SS.reconfigure forSampling @01:02:34.92 SS.seek 25.00ml @01:02:48.78 Stroke completed -- waiting for pressure to equalize ... @01:02:49.42 Intake.select :close @01:02:49.57 SSV.dial :exhaust @01:02:50.35 Exhaust.select :open @01:02:50.45 SS.reconfigure SSexhale @01:02:50.50 SS.seek 0.00ml @01:02:59.20 Exhaust.select :close @01:02:59.31 SSV.dial CBV @01:02:59.33 CBV.dial SSV @01:03:00.09 Allowing 185s for 25.0ml of remaining 625.0ml @01:03:00.11 Intake.select :open @01:03:02.26 Intake @14.4psia, vacuum from 7.9 to 11.9psi @01:03:02.28 SS.reconfigure forSampling @01:03:02.34 SS.seek 25.00ml @01:03:16.21 Stroke completed -- waiting for pressure to equalize ... @01:03:16.84 Intake.select :close @01:03:16.98 SSV.dial :exhaust @01:03:17.76 Exhaust.select :open @01:03:17.86 SS.reconfigure SSexhale @01:03:17.91 SS.seek 0.00ml @01:03:26.70 Exhaust.select :close @01:03:26.80 SSV.dial CBV @01:03:26.82 CBV.dial SSV @01:03:27.60 Allowing 185s for 25.0ml of remaining 600.0ml @01:03:27.62 Intake.select :open @01:03:30.05 Intake @14.4psia, vacuum from 7.9 to 11.9psi @01:03:30.06 SS.reconfigure forSampling @01:03:30.11 SS.seek 25.00ml @01:03:43.98 Stroke completed -- waiting for pressure to equalize ... @01:03:44.61 Intake.select :close @01:03:44.75 SSV.dial :exhaust @01:03:45.52 Exhaust.select :open @01:03:45.62 SS.reconfigure SSexhale @01:03:45.67 SS.seek 0.00ml @01:03:54.40 Exhaust.select :close @01:03:54.50 SSV.dial CBV @01:03:54.52 CBV.dial SSV @01:03:55.30 Allowing 185s for 25.0ml of remaining 575.0ml @01:03:55.32 Intake.select :open @01:03:57.46 Intake @14.4psia, vacuum from 7.9 to 11.9psi @01:03:57.48 SS.reconfigure forSampling @01:03:57.54 SS.seek 25.00ml @01:04:11.90 Stroke completed -- waiting for pressure to equalize ... @01:04:12.54 Intake.select :close @01:04:12.68 SSV.dial :exhaust @01:04:13.46 Exhaust.select :open @01:04:13.56 SS.reconfigure SSexhale @01:04:13.61 SS.seek 0.00ml @01:04:22.49 Exhaust.select :close @01:04:22.59 SSV.dial CBV @01:04:22.61 CBV.dial SSV @01:04:23.38 Allowing 185s for 25.0ml of remaining 550.0ml @01:04:23.40 Intake.select :open @01:04:25.55 Intake @14.4psia, vacuum from 7.9 to 11.9psi @01:04:25.57 SS.reconfigure forSampling @01:04:25.63 SS.seek 25.00ml @01:04:39.41 Stroke completed -- waiting for pressure to equalize ... @01:04:40.05 Intake.select :close @01:04:40.19 SSV.dial :exhaust @01:04:40.96 Exhaust.select :open @01:04:41.06 SS.reconfigure SSexhale @01:04:41.11 SS.seek 0.00ml @01:04:49.77 Exhaust.select :close @01:04:49.87 SSV.dial CBV @01:04:49.89 CBV.dial SSV @01:04:50.66 Allowing 185s for 25.0ml of remaining 525.0ml @01:04:50.68 Intake.select :open @01:04:52.82 Intake @14.4psia, vacuum from 7.9 to 11.9psi @01:04:52.84 SS.reconfigure forSampling @01:04:52.90 SS.seek 25.00ml @01:05:06.77 Stroke completed -- waiting for pressure to equalize ... @01:05:07.41 Intake.select :close @01:05:07.55 SSV.dial :exhaust @01:05:08.32 Exhaust.select :open @01:05:08.43 SS.reconfigure SSexhale @01:05:08.48 SS.seek 0.00ml @01:05:17.42 Exhaust.select :close @01:05:17.52 SSV.dial CBV @01:05:17.54 CBV.dial SSV @01:05:18.31 Allowing 185s for 25.0ml of remaining 500.0ml @01:05:18.33 Intake.select :open @01:05:20.48 Intake @14.5psia, vacuum from 8.0 to 12.0psi @01:05:20.50 SS.reconfigure forSampling @01:05:20.56 SS.seek 25.00ml @01:05:34.39 Stroke completed -- waiting for pressure to equalize ... @01:05:35.02 Intake.select :close @01:05:35.17 SSV.dial :exhaust @01:05:35.95 Exhaust.select :open @01:05:36.05 SS.reconfigure SSexhale @01:05:36.10 SS.seek 0.00ml @01:05:45.06 Exhaust.select :close @01:05:45.16 SSV.dial CBV @01:05:45.18 CBV.dial SSV @01:05:45.95 Allowing 185s for 25.0ml of remaining 475.0ml @01:05:45.97 Intake.select :open @01:05:48.11 Intake @14.3psia, vacuum from 7.8 to 11.8psi @01:05:48.13 SS.reconfigure forSampling @01:05:48.18 SS.seek 25.00ml @01:06:01.98 Stroke completed -- waiting for pressure to equalize ... @01:06:02.61 Intake.select :close @01:06:02.76 SSV.dial :exhaust @01:06:03.55 Exhaust.select :open @01:06:03.65 SS.reconfigure SSexhale @01:06:03.70 SS.seek 0.00ml @01:06:12.62 Exhaust.select :close @01:06:12.72 SSV.dial CBV @01:06:12.74 CBV.dial SSV @01:06:13.53 Allowing 185s for 25.0ml of remaining 450.0ml @01:06:13.54 Intake.select :open @01:06:15.68 Intake @14.3psia, vacuum from 7.8 to 11.8psi @01:06:15.70 SS.reconfigure forSampling @01:06:15.76 SS.seek 25.00ml @01:06:29.59 Stroke completed -- waiting for pressure to equalize ... @01:06:30.22 Intake.select :close @01:06:30.36 SSV.dial :exhaust @01:06:31.14 Exhaust.select :open @01:06:31.24 SS.reconfigure SSexhale @01:06:31.29 SS.seek 0.00ml @01:06:40.06 Exhaust.select :close @01:06:40.17 SSV.dial CBV @01:06:40.19 CBV.dial SSV @01:06:40.97 Allowing 185s for 25.0ml of remaining 425.0ml @01:06:40.99 Intake.select :open @01:06:43.13 Intake @14.4psia, vacuum from 7.9 to 11.9psi @01:06:43.15 SS.reconfigure forSampling @01:06:43.21 SS.seek 25.00ml @01:06:57.03 Stroke completed -- waiting for pressure to equalize ... @01:06:57.66 Intake.select :close @01:06:57.80 SSV.dial :exhaust @01:06:58.58 Exhaust.select :open @01:06:58.68 SS.reconfigure SSexhale @01:06:58.73 SS.seek 0.00ml @01:07:07.89 Exhaust.select :close @01:07:07.99 SSV.dial CBV @01:07:08.01 CBV.dial SSV @01:07:08.80 Allowing 185s for 25.0ml of remaining 400.0ml @01:07:08.81 Intake.select :open @01:07:10.96 Intake @14.4psia, vacuum from 7.9 to 11.9psi @01:07:10.98 SS.reconfigure forSampling @01:07:11.04 SS.seek 25.00ml @01:07:24.93 Stroke completed -- waiting for pressure to equalize ... @01:07:25.57 Intake.select :close @01:07:25.72 SSV.dial :exhaust @01:07:26.51 Exhaust.select :open @01:07:26.61 SS.reconfigure SSexhale @01:07:26.66 SS.seek 0.00ml @01:07:35.48 Exhaust.select :close @01:07:35.58 SSV.dial CBV @01:07:35.60 CBV.dial SSV @01:07:36.37 Allowing 185s for 25.0ml of remaining 375.0ml @01:07:36.39 Intake.select :open @01:07:38.53 Intake @14.4psia, vacuum from 7.9 to 11.9psi @01:07:38.55 SS.reconfigure forSampling @01:07:38.61 SS.seek 25.00ml @01:07:52.45 Stroke completed -- waiting for pressure to equalize ... @01:07:53.08 Intake.select :close @01:07:53.22 SSV.dial :exhaust @01:07:53.99 Exhaust.select :open @01:07:54.09 SS.reconfigure SSexhale @01:07:54.14 SS.seek 0.00ml @01:08:03.07 Exhaust.select :close @01:08:03.17 SSV.dial CBV @01:08:03.19 CBV.dial SSV @01:08:03.95 Allowing 185s for 25.0ml of remaining 350.0ml @01:08:03.97 Intake.select :open @01:08:06.12 Intake @14.4psia, vacuum from 7.9 to 11.9psi @01:08:06.14 SS.reconfigure forSampling @01:08:06.20 SS.seek 25.00ml @01:08:20.43 Stroke completed -- waiting for pressure to equalize ... @01:08:21.06 Intake.select :close @01:08:21.21 SSV.dial :exhaust @01:08:21.99 Exhaust.select :open @01:08:22.09 SS.reconfigure SSexhale @01:08:22.14 SS.seek 0.00ml @01:08:30.88 Exhaust.select :close @01:08:30.98 SSV.dial CBV @01:08:31.00 CBV.dial SSV @01:08:31.78 Allowing 185s for 25.0ml of remaining 325.0ml @01:08:31.79 Intake.select :open @01:08:33.94 Intake @14.2psia, vacuum from 7.7 to 11.7psi @01:08:33.96 SS.reconfigure forSampling @01:08:34.01 SS.seek 25.00ml @01:08:47.76 Stroke completed -- waiting for pressure to equalize ... @01:08:48.39 Intake.select :close @01:08:48.53 SSV.dial :exhaust @01:08:49.30 Exhaust.select :open @01:08:49.40 SS.reconfigure SSexhale @01:08:49.45 SS.seek 0.00ml @01:08:58.43 Exhaust.select :close @01:08:58.54 SSV.dial CBV @01:08:58.56 CBV.dial SSV @01:08:59.33 Allowing 185s for 25.0ml of remaining 300.0ml @01:08:59.35 Intake.select :open @01:09:01.50 Intake @14.4psia, vacuum from 7.9 to 11.9psi @01:09:01.52 SS.reconfigure forSampling @01:09:01.58 SS.seek 25.00ml @01:09:15.41 Stroke completed -- waiting for pressure to equalize ... @01:09:16.04 Intake.select :close @01:09:16.18 SSV.dial :exhaust @01:09:16.94 Exhaust.select :open @01:09:17.04 SS.reconfigure SSexhale @01:09:17.09 SS.seek 0.00ml @01:09:26.08 Exhaust.select :close @01:09:26.18 SSV.dial CBV @01:09:26.20 CBV.dial SSV @01:09:26.97 Allowing 185s for 25.0ml of remaining 275.0ml @01:09:26.99 Intake.select :open @01:09:29.13 Intake @14.3psia, vacuum from 7.8 to 11.8psi @01:09:29.15 SS.reconfigure forSampling @01:09:29.21 SS.seek 25.00ml @01:09:43.05 Stroke completed -- waiting for pressure to equalize ... @01:09:43.68 Intake.select :close @01:09:43.82 SSV.dial :exhaust @01:09:44.71 Exhaust.select :open @01:09:44.82 SS.reconfigure SSexhale @01:09:44.87 SS.seek 0.00ml @01:09:53.83 Exhaust.select :close @01:09:53.93 SSV.dial CBV @01:09:53.95 CBV.dial SSV @01:09:54.72 Allowing 185s for 25.0ml of remaining 250.0ml @01:09:54.74 Intake.select :open @01:09:56.88 Intake @14.3psia, vacuum from 7.8 to 11.8psi @01:09:56.90 SS.reconfigure forSampling @01:09:56.95 SS.seek 25.00ml @01:10:10.80 Stroke completed -- waiting for pressure to equalize ... @01:10:11.44 Intake.select :close @01:10:11.58 SSV.dial :exhaust @01:10:12.35 Exhaust.select :open @01:10:12.45 SS.reconfigure SSexhale @01:10:12.50 SS.seek 0.00ml @01:10:21.78 Exhaust.select :close @01:10:21.88 SSV.dial CBV @01:10:21.90 CBV.dial SSV @01:10:22.67 Allowing 185s for 25.0ml of remaining 225.0ml @01:10:22.69 Intake.select :open @01:10:24.84 Intake @14.4psia, vacuum from 7.9 to 11.9psi @01:10:24.86 SS.reconfigure forSampling @01:10:24.92 SS.seek 25.00ml @01:10:38.67 Stroke completed -- waiting for pressure to equalize ... @01:10:39.31 Intake.select :close @01:10:39.45 SSV.dial :exhaust @01:10:40.22 Exhaust.select :open @01:10:40.32 SS.reconfigure SSexhale @01:10:40.37 SS.seek 0.00ml @01:10:49.25 Exhaust.select :close @01:10:49.36 SSV.dial CBV @01:10:49.38 CBV.dial SSV @01:10:50.16 Allowing 185s for 25.0ml of remaining 200.0ml @01:10:50.18 Intake.select :open @01:10:52.32 Intake @14.4psia, vacuum from 7.9 to 11.9psi @01:10:52.34 SS.reconfigure forSampling @01:10:52.40 SS.seek 25.00ml @01:11:06.28 Stroke completed -- waiting for pressure to equalize ... @01:11:06.92 Intake.select :close @01:11:07.06 SSV.dial :exhaust @01:11:07.84 Exhaust.select :open @01:11:07.94 SS.reconfigure SSexhale @01:11:07.99 SS.seek 0.00ml @01:11:16.66 Exhaust.select :close @01:11:16.76 SSV.dial CBV @01:11:16.78 CBV.dial SSV @01:11:17.58 Allowing 185s for 25.0ml of remaining 175.0ml @01:11:17.60 Intake.select :open @01:11:19.74 Intake @14.4psia, vacuum from 7.9 to 11.9psi @01:11:19.76 SS.reconfigure forSampling @01:11:19.82 SS.seek 25.00ml @01:11:34.05 Stroke completed -- waiting for pressure to equalize ... @01:11:34.69 Intake.select :close @01:11:34.83 SSV.dial :exhaust @01:11:35.61 Exhaust.select :open @01:11:35.71 SS.reconfigure SSexhale @01:11:35.76 SS.seek 0.00ml @01:11:44.56 Exhaust.select :close @01:11:44.67 SSV.dial CBV @01:11:44.69 CBV.dial SSV @01:11:45.45 Allowing 185s for 25.0ml of remaining 150.0ml @01:11:45.47 Intake.select :open @01:11:47.62 Intake @14.4psia, vacuum from 7.9 to 11.9psi @01:11:47.64 SS.reconfigure forSampling @01:11:47.69 SS.seek 25.00ml @01:12:01.51 Stroke completed -- waiting for pressure to equalize ... @01:12:02.15 Intake.select :close @01:12:02.29 SSV.dial :exhaust @01:12:03.08 Exhaust.select :open @01:12:03.18 SS.reconfigure SSexhale @01:12:03.23 SS.seek 0.00ml @01:12:12.11 Exhaust.select :close @01:12:12.21 SSV.dial CBV @01:12:12.23 CBV.dial SSV @01:12:13.01 Allowing 185s for 25.0ml of remaining 125.0ml @01:12:13.03 Intake.select :open @01:12:15.18 Intake @14.3psia, vacuum from 7.8 to 11.8psi @01:12:15.20 SS.reconfigure forSampling @01:12:15.25 SS.seek 25.00ml @01:12:29.14 Stroke completed -- waiting for pressure to equalize ... @01:12:29.82 Intake.select :close @01:12:29.96 SSV.dial :exhaust @01:12:30.75 Exhaust.select :open @01:12:30.85 SS.reconfigure SSexhale @01:12:30.90 SS.seek 0.00ml @01:12:39.67 Exhaust.select :close @01:12:39.77 SSV.dial CBV @01:12:39.79 CBV.dial SSV @01:12:40.56 Allowing 185s for 25.0ml of remaining 100.0ml @01:12:40.58 Intake.select :open @01:12:42.73 Intake @14.3psia, vacuum from 7.8 to 11.8psi @01:12:42.74 SS.reconfigure forSampling @01:12:42.80 SS.seek 25.00ml @01:12:56.66 Stroke completed -- waiting for pressure to equalize ... @01:12:57.29 Intake.select :close @01:12:57.43 SSV.dial :exhaust @01:12:58.19 Exhaust.select :open @01:12:58.29 SS.reconfigure SSexhale @01:12:58.34 SS.seek 0.00ml @01:13:07.60 Exhaust.select :close @01:13:07.70 SSV.dial CBV @01:13:07.72 CBV.dial SSV @01:13:08.49 Allowing 185s for 25.0ml of remaining 75.0ml @01:13:08.51 Intake.select :open @01:13:10.65 Intake @14.4psia, vacuum from 7.9 to 11.9psi @01:13:10.67 SS.reconfigure forSampling @01:13:10.73 SS.seek 25.00ml @01:13:24.57 Stroke completed -- waiting for pressure to equalize ... @01:13:25.21 Intake.select :close @01:13:25.35 SSV.dial :exhaust @01:13:26.13 Exhaust.select :open @01:13:26.23 SS.reconfigure SSexhale @01:13:26.28 SS.seek 0.00ml @01:13:35.25 Exhaust.select :close @01:13:35.35 SSV.dial CBV @01:13:35.37 CBV.dial SSV @01:13:36.15 Allowing 185s for 25.0ml of remaining 50.0ml @01:13:36.16 Intake.select :open @01:13:38.31 Intake @14.4psia, vacuum from 7.9 to 11.9psi @01:13:38.33 SS.reconfigure forSampling @01:13:38.39 SS.seek 25.00ml @01:13:52.56 Stroke completed -- waiting for pressure to equalize ... @01:13:53.19 Intake.select :close @01:13:53.33 SSV.dial :exhaust @01:13:54.11 Exhaust.select :open @01:13:54.21 SS.reconfigure SSexhale @01:13:54.26 SS.seek 0.00ml @01:14:03.00 Exhaust.select :close @01:14:03.10 SSV.dial CBV @01:14:03.12 CBV.dial SSV @01:14:03.89 Allowing 185s for 25.0ml of remaining 25.0ml @01:14:03.91 Intake.select :open @01:14:06.05 Intake @14.3psia, vacuum from 7.8 to 11.8psi @01:14:06.07 SS.reconfigure forSampling @01:14:06.13 SS.seek 24.99ml @01:14:19.94 Stroke completed -- waiting for pressure to equalize ... @01:14:20.57 Intake.select :close @01:14:20.73 SSV.dial :exhaust @01:14:21.52 Exhaust.select :open @01:14:21.63 SS.reconfigure SSexhale @01:14:21.68 SS.seek 0.00ml @01:14:31.00 Exhaust.select :close @01:14:31.11 Sampled 1000.0ml @01:14:31.12 collectSample: Complete @01:14:31.14 Drying puck before fixing for 04:00.00 @01:14:31.15 CBV.dial :waste2b @01:14:31.93 delay 1:00 @01:14:34.10 Sleepy.power :sampler,:OFF @01:15:31.93 CSV.dial :air @01:15:32.47 CS.seek 10.000ml @01:15:55.95 CBV.dial between CSV and waste2b @01:15:56.28 Pumping wcr puck from tube 4 up to 55psi... @01:15:56.34 CBV.dial SSV @01:15:57.77 CBV.dial between CSV and waste2b @01:15:58.71 CSV.dial between air and CTV @01:15:59.11 CS.seek 7.270ml @01:16:06.05 CTV.dial CSV,avoiding => air @01:16:06.07 CSV.dial CTV @01:16:06.83 CS.reconfigure CSpressurize @01:16:06.88 CS.seek 0.000ml @01:16:25.85 CSV.dial :air @01:16:26.38 CS.reconfigure CSfast @01:16:26.42 CS.seek 10.000ml @01:16:49.70 CSV.dial between air and CTV @01:16:50.08 CS.seek 2.749ml @01:17:07.20 CTV.dial CSV,avoiding => air @01:17:07.21 CSV.dial CTV @01:17:07.53 CS.reconfigure CSpressurize @01:17:07.58 CS.seek 0.000ml @01:17:13.37 CBV.dial :waste2b @01:17:13.70 CS.reconfigure CSconfig @01:17:13.74 CS.seek empty @01:17:16.16 delay 1:00 @01:18:16.17 CTV.dial :air,avoiding => intake @01:18:17.45 Fix #1 of 2 (with rnal) for 02:00.00 @01:18:17.46 CS Pulling 2ml of rnal @01:18:17.47 CSV.dial CSR @01:18:17.92 CSR.select :rnal @01:18:18.03 CS.seek 2.000ml @01:18:24.00 delay 5 seconds @01:18:29.01 CSR.select :air @01:18:29.12 CS.reconfigure CSfast @01:18:29.17 CS.seek 10.000ml @01:18:48.44 CBV.dial between CSV and waste2b @01:18:48.78 Pumping wcr puck from tube 4 up to 55psi... @01:18:48.84 CBV.dial SSV @01:18:50.27 CBV.dial between CSV and waste2b @01:18:51.22 CSV.dial between air and CTV @01:18:51.89 CS.seek 7.311ml @01:18:58.73 CTV.dial CSV,avoiding => air @01:18:58.75 CSV.dial CTV @01:19:00.07 CS.reconfigure CSpressurize @01:19:00.12 CS.seek 0.000ml @01:19:18.74 delay 2:00 @01:21:18.75 CBV.dial :waste2b @01:21:19.08 CS.reconfigure CSconfig @01:21:19.12 CS.seek empty @01:21:20.16 delay 1:00 @01:22:20.16 CTV.dial :air,avoiding => intake @01:22:21.44 Fix #2 of 2 (with rnal) for 02:00.00 @01:22:21.45 CS Pulling 2ml of rnal @01:22:21.46 CSV.dial CSR @01:22:21.91 CSR.select :rnal @01:22:22.02 CS.seek 2.000ml @01:22:27.99 delay 5 seconds @01:22:33.01 CSR.select :air @01:22:33.12 CS.reconfigure CSfast @01:22:33.17 CS.seek 10.000ml @01:22:51.83 CBV.dial between CSV and waste2b @01:22:52.17 Pumping wcr puck from tube 4 up to 55psi... @01:22:52.23 CBV.dial SSV @01:22:53.65 CBV.dial between CSV and waste2b @01:22:54.60 CSV.dial between air and CTV @01:22:55.26 CS.seek 7.193ml @01:23:02.37 CTV.dial CSV,avoiding => air @01:23:02.39 CSV.dial CTV @01:23:03.75 CS.reconfigure CSpressurize @01:23:03.80 CS.seek 0.000ml @01:23:22.04 delay 2:00 @01:25:22.04 CBV.dial :waste2b @01:25:22.37 CS.reconfigure CSconfig @01:25:22.41 CS.seek empty @01:25:23.55 delay 1:00 @01:26:23.56 CTV.dial :air,avoiding => intake @01:26:24.87 Storing wcr puck in Collection Clamp to tube 3 @01:26:24.98 SE.seek down @01:26:25.28 SC.reconfigure SCtight @01:26:25.33 SC.seek 3 @01:26:27.55 SC.reconfigure SCloose @01:26:27.73 Elbow.reconfigure TightElbow @01:26:27.78 Elbow.seek 3 @01:26:29.40 Forearm.reconfigure TightForearm @01:26:29.45 Forearm.seek 3 + 800 counts @01:26:30.42 SE.reconfigure SEpick @01:26:30.49 SE.seek beamBreak @01:26:33.28 SE.configure SEconfig @01:26:33.42 Elbow.reconfigure LimpElbow @01:26:33.46 Forearm.reconfigure LooseForearm @01:26:33.51 Forearm.seek 3 @01:26:33.75 Elbow.hold @01:26:33.81 Elbow.reconfigure TightElbow @01:26:33.90 SE.seek 2.06 Pucks below Top Plate @01:26:34.21 Forearm.coast @01:26:34.26 Forearm.seek 3 + 345 counts @01:26:34.58 Forearm.reconfigure TightForearm @01:26:34.63 Forearm.seek 3 + 800 counts @01:26:34.90 SE.seek 3.06 Pucks below Top Plate @01:26:35.98 Forearm.reconfigure LooseForearm @01:26:36.03 Forearm.seek retract @01:26:36.96 Elbow.seek CC @01:26:38.55 Elbow.reconfigure LooseElbow @01:26:38.64 Forearm.seek CC @01:26:39.61 CC.seek sealed @01:26:40.54 Hand.grip @01:26:41.29 CC.seek open @01:26:44.64 Forearm.seek retract @01:26:45.61 Elbow.reconfigure TightElbow @01:26:45.66 Elbow.seek 3 @01:26:47.30 Elbow.reconfigure LimpElbow @01:26:47.36 Forearm.seek 3 @01:26:48.35 Elbow.hold @01:26:48.42 Elbow.reconfigure TightElbow @01:26:48.47 Hand.release @01:26:49.23 Forearm.coast @01:26:49.29 Forearm.seek 3 + 346 counts @01:26:49.57 Forearm.reconfigure TightForearm @01:26:49.63 Forearm.seek 3 + 800 counts @01:26:49.96 SE.seek down @01:26:51.05 Forearm.reconfigure LooseForearm @01:26:51.10 Forearm.seek retract @01:26:52.35 SE.coast @01:26:52.39 SC.coast @01:26:52.43 Starting finalPurge @01:26:52.44 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @01:26:52.61 Elbow.seek FlushPuck::Garage @01:26:54.42 Elbow.reconfigure LooseElbow @01:26:54.52 Forearm.seek garage @01:26:55.57 Hand.grip @01:26:56.37 Forearm.seek retract @01:26:57.43 Elbow.reconfigure TightElbow @01:26:57.48 Elbow.seek CC @01:27:00.25 Elbow.reconfigure LooseElbow @01:27:00.35 Forearm.reconfigure TightForearm @01:27:00.39 Forearm.seek CC @01:27:01.51 CC.seek closed @01:27:05.32 Collection Clamp onSpring/offSpring for 5/21 samples, on/off torque 2.03 @01:27:05.38 Hand.release @01:27:06.20 Forearm.reconfigure LooseForearm @01:27:06.24 Forearm.seek retract @01:27:07.21 Flushing the Collection Valve::Manifold @01:27:07.32 CS Pulling 5ml of flush @01:27:07.33 CSV.dial CSR @01:27:07.84 CSR.select :flush @01:27:07.95 CS.seek 5.000ml @01:27:21.54 delay 5 seconds @01:27:26.55 CSR.select :air @01:27:26.71 CS.reconfigure CSfast @01:27:26.75 CS.seek 7.501ml @01:27:33.26 CSV.dial :waste @01:27:34.52 CS.reconfigure CSconfig @01:27:34.57 CS.seek 0.000ml @01:27:54.41 Flushing lower manifold's waste1 port @01:27:55.08 CSV.dial :flush @01:27:55.52 CS.reconfigure CSfast @01:27:55.56 CS.seek 5.000ml @01:28:07.57 delay 5 seconds @01:28:12.58 CSV.dial :air @01:28:13.07 CS.seek 7.000ml @01:28:18.41 CSV.dial CTV @01:28:18.43
CTV.dial CSV @01:28:19.76 CBV.dial :waste1 @01:28:20.50 CS.seek 0.000ml @01:28:36.82 Flushing lower manifold's waste2b port @01:28:36.93 CSV.dial :flush @01:28:37.73 CS.seek 5.000ml @01:28:49.88 delay 5 seconds @01:28:54.89 CSV.dial :air @01:28:55.46 CS.seek 7.000ml @01:29:00.88 CSV.dial CTV @01:29:00.90
CTV.dial CSV @01:29:01.36 CBV.dial :waste2b @01:29:02.11 CS.seek 0.000ml @01:29:18.44 Flushing lower manifold's waste1 port @01:29:18.45 CS Pulling 4ml of mfbkill @01:29:18.47 CSV.dial CSR @01:29:18.91 CSR.select :mfbkill @01:29:19.02 CS.reconfigure CSconfig @01:29:19.07 CS.seek 4.000ml @01:29:30.07 delay 5 seconds @01:29:35.08 CSR.select :air @01:29:35.19 CS.reconfigure CSfast @01:29:35.24 CS.seek 10.000ml @01:29:49.58 CSV.dial CTV @01:29:49.61
CTV.dial CSV @01:29:50.13 CBV.dial :waste1 @01:29:50.88 CS.seek 0.000ml @01:30:13.95 Flushing lower manifold's waste2b port @01:30:13.96 CS Pulling 4ml of mfbkill @01:30:13.97 CSV.dial CSR @01:30:14.50 CSR.select :mfbkill @01:30:14.61 CS.reconfigure CSconfig @01:30:14.66 CS.seek 4.000ml @01:30:25.67 delay 5 seconds @01:30:30.68 CSR.select :air @01:30:30.79 CS.reconfigure CSfast @01:30:30.84 CS.seek 10.000ml @01:30:45.04 CSV.dial CTV @01:30:45.06
CTV.dial CSV @01:30:45.60 CBV.dial :waste2b @01:30:46.33 CS.seek 0.000ml @01:31:09.29 Killing upper manifold @01:31:09.30 CS Pulling 4ml of mfbkill @01:31:09.31 CSV.dial CSR @01:31:09.82 CSR.select :mfbkill @01:31:09.92 CS.reconfigure CSconfig @01:31:09.97 CS.seek 4.000ml @01:31:20.88 delay 5 seconds @01:31:25.89 CSR.select :air @01:31:25.99 CS.reconfigure CSfast @01:31:26.04 CS.seek 10.000ml @01:31:40.23 CSV.dial CBV @01:31:40.25
CBV.dial CSV @01:31:41.29 CTV.dial :air @01:31:42.60 CS.seek 0.000ml @01:32:05.51 delay 7 seconds @01:32:12.52 CS.seek 3.000ml @01:32:20.22 CSV.dial :waste @01:32:20.67 CS.seek 0.000ml @01:32:28.22 Flushing upper manifold @01:32:28.33 CSV.dial :flush @01:32:28.77 CS.seek 5.000ml @01:32:40.75 delay 5 seconds @01:32:45.76 CSV.dial :air @01:32:46.24 CS.seek 7.000ml @01:32:51.66 CSV.dial CBV @01:32:51.68
CBV.dial CSV @01:32:52.77 CTV.dial :air @01:32:52.79 CS.seek 0.000ml @01:33:09.22 delay 7 seconds @01:33:16.23 CS.seek 3.000ml @01:33:23.75 CSV.dial :waste @01:33:24.28 CS.seek 0.000ml @01:33:31.90 CSV.dial :flush @01:33:32.34 CS.seek 3.000ml @01:33:39.97 delay 5 seconds @01:33:44.98 CSV.dial :air @01:33:45.46 CS.seek 5.000ml @01:33:50.81 CSV.dial :air @01:33:50.82 CS.seek 0.000ml @01:34:02.75 delay 7 seconds @01:34:09.76 CS.seek 3.000ml @01:34:17.34 CSV.dial :waste @01:34:18.15 CS.seek 0.000ml @01:34:25.84 Flushing lower manifold's waste1 port @01:34:25.95 CSV.dial :flush @01:34:26.93 CS.seek 5.000ml @01:34:38.91 delay 5 seconds @01:34:43.92 CSV.dial :air @01:34:44.41 CS.seek 7.000ml @01:34:49.75 CSV.dial CTV @01:34:49.77
CTV.dial CSV @01:34:51.12 CBV.dial :waste1 @01:34:51.59 CS.seek 0.000ml @01:35:07.94 Flushing lower manifold's waste2b port @01:35:08.05 CSV.dial :flush @01:35:08.85 CS.seek 5.000ml @01:35:20.84 delay 5 seconds @01:35:25.85 CSV.dial :air @01:35:26.41 CS.seek 6.999ml @01:35:31.68 CSV.dial CTV @01:35:31.70
CTV.dial CSV @01:35:32.14 CBV.dial :waste2b @01:35:32.90 CS.seek 0.000ml @01:35:49.22 Garaging FlushPuck in Collection Clamp @01:35:49.29 Elbow.reconfigure TightElbow @01:35:49.34 Elbow.seek CC @01:35:49.45 Elbow.reconfigure LooseElbow @01:35:49.54 Forearm.seek CC @01:35:50.52 CC.seek sealed @01:35:51.43 Hand.grip @01:35:52.15 CC.seek open @01:35:55.46 Forearm.seek retract @01:35:56.42 Elbow.reconfigure TightElbow @01:35:56.47 Elbow.seek garage @01:35:59.28 Elbow.reconfigure LooseElbow @01:35:59.37 Forearm.reconfigure TightForearm @01:35:59.41 Forearm.seek garage @01:36:00.56 Hand.release @01:36:01.31 Forearm.reconfigure LooseForearm @01:36:01.35 Forearm.seek retract @01:36:02.40 Completed {archivestack} begun 23:31:37 @01:36:02.41 CC.stop @01:36:02.44
Asleep after completing 14mar19sccwrp assays @01:36:02.48 GoodNews.email "",Subject => "Asleep after completing 14mar19sccwrp assays" @01:36:03.51 CS.stop @01:36:03.55 PC.stop @01:36:03.59 Forearm.stop @01:36:03.63 Elbow.stop @01:36:03.66 SS.stop @01:36:03.72 Sleepy.power :raw,:OFF @01:36:03.74 Sleepy.power :dwarf,:OFF @01:36:03.75 Shutdown @01:36:03.76
delayUntil "06:00:00.00" @06:00:00.00 GoodNews.email "neo completed 14mar19sccwrp mission",Subject => "Ready to Recover" @06:00:11.36 Session Ended