@19:00:00.00EST31-Dec-69 Configuration for niagara by engrstrom@mclanelabs.com /home/esp/esp2/shallow/niagara/configure.rb All dwarf controllers running firmware version 3.99 @19:00:00.06 Loading /home/esp/esp2/mission/niagara18Aug14phaserunQC.rb mission CTD.latitude = 36.8044 Degrees North in seawater SensorPolling::Error in pollContext -- No CTD.playback file specified Preparing to sleep until 1:35PM 8/14/18 Asleep til 1:35PM 8/14/18 Continuing at 13:35EDT14-Aug-18 @19:00:00.36 Shutdown @13:35:15.41EDT14-Aug-18 Phase [da withoutWCR] da sampling at most 100ml Loading sh1 puck from tube 6 into Collection Clamp @13:35:36.27 Tube 6 was empty. Trying tube 7 ... Loading sh1 puck from tube 7 into Collection Clamp @13:35:47.98 Storage::Error in quick -- Out of Pucks Waiting to Resume from Checkpoint "Loading sh1 puck from tube 7 into Collection Clamp" @19:00:00.00EST31-Dec-69 Configuration for niagara by engrstrom@mclanelabs.com /home/esp/esp2/shallow/niagara/configure.rb All dwarf controllers running firmware version 3.99 @19:00:00.06 Loading /home/esp/esp2/mission/2018_07_17_niagara_postcanQC.rb mission Preparing to sleep until 3:30PM 8/24/18 Asleep til 3:30PM 8/24/18 Continuing at 15:30EDT24-Aug-18 @19:00:00.36 Shutdown @15:30:15.41EDT24-Aug-18 Phase [da withoutWCR {noKill}] da sampling at most 150ml Loading sh1 puck from tube 3 into Collection Clamp @15:30:26.69 Tube 3 was empty. Trying tube 4 ... Loading sh1 puck from tube 4 into Collection Clamp @15:30:37.51 Tube 4 was empty. Trying tube 5 ... Loading sh1 puck from tube 5 into Collection Clamp @15:30:48.32 Tube 5 was empty. Trying tube 6 ... Loading sh1 puck from tube 6 into Collection Clamp @15:30:59.14 Tube 6 was empty. Trying tube 7 ... Loading sh1 puck from tube 7 into Collection Clamp @15:31:10.85 Storage::Error in quick -- Out of Pucks Waiting to Resume from Checkpoint "Loading sh1 puck from tube 7 into Collection Clamp" @19:00:00.00EST31-Dec-69 Configuration for niagara by engrstrom@mclanelabs.com /home/esp/esp2/shallow/niagara/configure.rb All dwarf controllers running firmware version 3.99 @19:00:00.06 Cleanly exited at 19:00:00EST31-Dec-69 Loading /home/esp/esp2/mission/2018_08_31_niagara_mission.rb mission Preparing to sleep until 11:30AM 8/31/2018 Asleep til 11:30AM 8/31/2018 Continuing at 11:30EDT31-Aug-18 @19:00:00.36 Shutdown @11:30:15.41EDT31-Aug-18 Phase [Sample.shallow; initialPurge; da withoutWCR] @11:30:16.91 Starting initialPurge Loading FlushPuck into Collection Clamp from FlushPuck::Garage @11:30:33.11 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @11:31:18.75 Purging external Intake @11:31:42.77 Flushing upper manifold @11:32:59.57 Garaging FlushPuck in Collection Clamp @11:33:12.67 da sampling at most 1000ml Loading sh1 puck from tube 2 into Collection Clamp @11:33:26.98 collectSample 1000ml w/bubble point of 24psi Priming 7ml @11:33:27.05 Calibrating pressure and temperature sensors @11:33:40.44 Waiting for external sampler to finish priming... @11:36:41.07 Sampling 1000.0ml @12:02:00.59 Sampled 1000.0ml Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @12:02:48.16 BubblePoint: Intake @14.7psia, Outlet @14.7psia @12:03:08.47 Evacuated: Intake @14.7psia, Outlet @14.7psia @12:03:36.53 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @12:04:22.60 BubblePoint: Intake @14.7psia, Outlet @14.7psia @12:04:42.92 Evacuated: Intake @14.7psia, Outlet @14.7psia @12:05:10.97 Lysing: Adding 2.0ml AqMeOH @12:06:18.79 Heating sh1 puck from tube 2 in Collection Clamp to 70C for 10:00 @12:17:44.79 Collection Puck Heater at 70C after lysing @12:18:26.16 Recovering Lysate into Collection syringe (recycling once) @12:19:07.34 Recycling Lysate (#1 of 1) @12:20:15.56 Recovering Lysate (#2 of 2) @12:20:56.74 Lysing Complete Storing sh1 puck in Collection Clamp to tube 1 @12:21:11.22 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:21:14.37 Tranferring DA Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:24:51.07 Garaging FlushPuck in Collection Clamp @12:25:04.17 Loading lyfil puck from tube 2 into Collection Clamp @12:27:32.60 Storing lyfil puck in Collection Clamp to tube 1 @12:27:41.58 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:27:55.69 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:28:58.53 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @12:30:16.08 Garaging FlushPuck in Processing Clamp @12:30:27.93 Loading da puck from tube 2 into Processing Clamp @12:30:47.06 Heating da puck from tube 2 in Processing Clamp to 28C for 2:00:00 @12:38:26.59 recoverPSRreagent08 @12:40:13.79 addPMLReagent08 @12:40:55.86 Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:44:32.43 Garaging FlushPuck in Collection Clamp @12:48:55.86 recoverPRVreagent08 @12:49:56.12 washCycleDA #1 of 2 @12:52:51.77 recoverPSRreagent08 @12:54:02.16 washCycleDA #2 of 2 @12:56:57.05 recoverPSRreagent08 @12:58:07.44 getPMLReagent08 @12:58:29.68 addPMLReagent08 @13:07:11.25 recoverPRVreagent08 @13:08:10.76 washCycleDA #1 of 3 @13:11:06.40 recoverPSRreagent08 @13:12:16.79 washCycleDA #2 of 3 @13:15:11.69 recoverPSRreagent08 @13:16:22.08 washCycleDA #3 of 3 @13:19:16.97 recoverPSRreagent08 @13:20:27.36 Mixing DA Substrates @13:21:38.10 recoverPSRreagent08 @13:22:48.49 Processing Puck Heater at 28C before imaging Imaging da puck in Processing Clamp while storing it to tube 1 @13:22:57.59 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @13:23:14.44 Lights, camera, ACTION! Simulating PuckCamera: snap -bin=2,2 -auto /var/log/esp/da18aug3113h1000ml.tif @13:23:34.44 Waiting >20 seconds for da to relinquish FlushPuck @13:25:04.44 Simulating PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da18aug3113h1000ml15s.tif @13:25:29.44 Simulating PuckCamera: snap -bin=2,2 30.0 /var/log/esp/midres/da18aug3113h1000ml30s.tif @13:26:09.44 Simulating PuckCamera: snap -bin=1,1 70.0 /var/log/esp/hires/da18aug3113h1000ml70s.tif @13:27:30.60 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @13:27:33.45 Starting finalPurge Loading FlushPuck into Processing Clamp from FlushPuck::Garage @13:27:53.45 Waiting >20 seconds for psFlush to relinquish FlushPuck @13:36:51.80 Loading FlushPuck into Collection Clamp from Processing Clamp @13:37:09.64 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @13:37:54.16 Flushing lower manifold's waste1 port @13:38:18.21 Flushing lower manifold's waste2 port @13:38:42.26 Disinfecting external Intake with kill @13:40:15.75 Garaging FlushPuck in Collection Clamp @13:40:28.85 Completed [Sample.shallow; initialPurge; da withoutWCR] begun 11:30:15 Preparing to sleep until 7:00AM 9/01/2018 Asleep til 7:00AM 9/01/2018 Continuing at 07:00EDT01-Sep-18 @13:40:29.15 Shutdown @07:00:08.26EDT01-Sep-18 Phase [Sample.deep; initialPurge; da withoutWCR] @07:00:09.76 Starting initialPurge Loading FlushPuck into Collection Clamp from FlushPuck::Garage @07:00:25.96 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @07:01:11.60 Purging external Intake @07:01:35.62 Flushing upper manifold @07:02:52.42 Garaging FlushPuck in Collection Clamp @07:03:05.52 da sampling at most 1000ml Loading sh1 puck from tube 2 into Collection Clamp @07:03:20.13 collectSample 1000ml w/bubble point of 24psi Priming 7ml @07:03:20.19 Calibrating pressure and temperature sensors @07:03:33.58 Waiting for external sampler to finish priming... @07:06:34.22 Sampling 1000.0ml @07:31:53.74 Sampled 1000.0ml Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @07:32:41.30 BubblePoint: Intake @14.7psia, Outlet @14.7psia @07:33:01.62 Evacuated: Intake @14.7psia, Outlet @14.7psia @07:33:29.67 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @07:34:15.75 BubblePoint: Intake @14.7psia, Outlet @14.7psia @07:34:36.06 Evacuated: Intake @14.7psia, Outlet @14.7psia @07:35:04.12 Lysing: Adding 2.0ml AqMeOH @07:36:11.94 Heating sh1 puck from tube 2 in Collection Clamp to 70C for 10:00 @07:47:37.94 Collection Puck Heater at 70C after lysing @07:48:19.31 Recovering Lysate into Collection syringe (recycling once) @07:49:00.49 Recycling Lysate (#1 of 1) @07:50:08.71 Recovering Lysate (#2 of 2) @07:50:49.89 Lysing Complete Storing sh1 puck in Collection Clamp to tube 1 @07:50:58.66 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @07:51:12.77 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @07:51:15.53 Tranferring DA Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @07:54:52.61 Garaging FlushPuck in Collection Clamp @07:55:05.71 Loading lyfil puck from tube 2 into Collection Clamp @07:57:34.44 Storing lyfil puck in Collection Clamp to tube 1 @07:57:43.12 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @07:57:57.23 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @07:58:59.77 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @08:00:18.33 Garaging FlushPuck in Processing Clamp @08:00:30.18 Loading da puck from tube 2 into Processing Clamp @08:00:49.61 Heating da puck from tube 2 in Processing Clamp to 28C for 2:00:00 @08:08:29.14 recoverPSRreagent08 @08:10:16.34 addPMLReagent08 @08:10:58.41 Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:14:34.97 Garaging FlushPuck in Collection Clamp @08:18:58.41 recoverPRVreagent08 @08:19:58.67 washCycleDA #1 of 2 @08:22:54.31 recoverPSRreagent08 @08:24:04.70 washCycleDA #2 of 2 @08:26:59.60 recoverPSRreagent08 @08:28:09.99 getPMLReagent08 @08:28:32.22 addPMLReagent08 @08:37:13.80 recoverPRVreagent08 @08:38:13.30 washCycleDA #1 of 3 @08:41:08.95 recoverPSRreagent08 @08:42:19.34 washCycleDA #2 of 3 @08:45:14.23 recoverPSRreagent08 @08:46:24.62 washCycleDA #3 of 3 @08:49:19.51 recoverPSRreagent08 @08:50:29.90 Mixing DA Substrates @08:51:40.64 recoverPSRreagent08 @08:52:51.03 Processing Puck Heater at 28C before imaging Imaging da puck in Processing Clamp while storing it to tube 1 @08:52:59.85 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:53:16.70 Lights, camera, ACTION! Simulating PuckCamera: snap -bin=2,2 -auto /var/log/esp/da18sep0108h1000ml.tif @08:53:36.70 Waiting >20 seconds for da to relinquish FlushPuck @08:55:06.70 Simulating PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da18sep0108h1000ml15s.tif @08:55:31.70 Simulating PuckCamera: snap -bin=2,2 30.0 /var/log/esp/midres/da18sep0108h1000ml30s.tif @08:56:11.70 Simulating PuckCamera: snap -bin=1,1 70.0 /var/log/esp/hires/da18sep0108h1000ml70s.tif @08:57:32.86 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:57:35.42 Loading FlushPuck into Processing Clamp from FlushPuck::Garage Starting finalPurge @08:57:55.42 Waiting >20 seconds for psFlush to relinquish FlushPuck @09:06:53.76 Loading FlushPuck into Collection Clamp from Processing Clamp @09:07:11.61 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @09:07:56.12 Flushing lower manifold's waste1 port @09:08:20.18 Flushing lower manifold's waste2 port @09:08:44.23 Disinfecting external Intake with kill @09:10:17.71 Garaging FlushPuck in Collection Clamp @09:10:30.81 Completed [Sample.deep; initialPurge; da withoutWCR] begun 07:00:08 Preparing to sleep until 11:30AM 9/01/2018 Asleep til 11:30AM 9/01/2018 Continuing at 11:30 @09:10:31.11 Shutdown @11:30:08.26 Phase [/home/esp/esp2/mission/2018_08_31_niagara_mission.rb:53] @11:30:09.76 Starting initialPurge Loading FlushPuck into Collection Clamp from FlushPuck::Garage @11:30:25.96 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @11:31:11.60 Purging external Intake @11:31:35.62 Flushing upper manifold @11:32:52.42 Garaging FlushPuck in Collection Clamp @11:33:05.52 da sampling at most 1000ml Loading sh1 puck from tube 2 into Collection Clamp @11:33:20.43 collectSample 1000ml w/bubble point of 24psi Priming 7ml @11:33:20.49 Calibrating pressure and temperature sensors @11:33:33.88 Waiting for external sampler to finish priming... @11:36:34.52 Sampling 1000.0ml @12:01:54.04 Sampled 1000.0ml Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @12:02:41.60 BubblePoint: Intake @14.7psia, Outlet @14.7psia @12:03:01.92 Evacuated: Intake @14.7psia, Outlet @14.7psia @12:03:29.97 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @12:04:16.04 BubblePoint: Intake @14.7psia, Outlet @14.7psia @12:04:36.36 Evacuated: Intake @14.7psia, Outlet @14.7psia @12:05:04.41 Lysing: Adding 2.0ml AqMeOH @12:06:12.24 Heating sh1 puck from tube 2 in Collection Clamp to 70C for 10:00 @12:17:38.24 Collection Puck Heater at 70C after lysing @12:18:19.60 Recovering Lysate into Collection syringe (recycling once) @12:19:00.78 Recycling Lysate (#1 of 1) @12:20:09.01 Recovering Lysate (#2 of 2) @12:20:50.19 Lysing Complete Storing sh1 puck in Collection Clamp to tube 1 @12:20:58.66 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:21:12.77 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:21:15.24 Tranferring DA Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:24:52.32 Garaging FlushPuck in Collection Clamp @12:25:05.42 Loading lyfil puck from tube 2 into Collection Clamp @12:27:34.44 Storing lyfil puck in Collection Clamp to tube 1 @12:27:42.82 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:27:56.93 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:28:59.18 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @12:30:17.74 Garaging FlushPuck in Processing Clamp @12:30:29.59 Loading da puck from tube 2 into Processing Clamp @12:30:49.31 Heating da puck from tube 2 in Processing Clamp to 28C for 2:00:00 @12:38:28.84 recoverPSRreagent08 @12:40:16.04 addPMLReagent08 @12:40:58.11 Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:44:34.68 Garaging FlushPuck in Collection Clamp @12:48:58.11 recoverPRVreagent08 @12:49:58.37 washCycleDA #1 of 2 @12:52:54.02 recoverPSRreagent08 @12:54:04.41 washCycleDA #2 of 2 @12:56:59.30 recoverPSRreagent08 @12:58:09.69 getPMLReagent08 @12:58:31.93 addPMLReagent08 @13:07:13.50 recoverPRVreagent08 @13:08:13.01 washCycleDA #1 of 3 @13:11:08.65 recoverPSRreagent08 @13:12:19.04 washCycleDA #2 of 3 @13:15:13.93 recoverPSRreagent08 @13:16:24.32 washCycleDA #3 of 3 @13:19:19.22 recoverPSRreagent08 @13:20:29.61 Mixing DA Substrates @13:21:40.35 recoverPSRreagent08 @13:22:50.74 Processing Puck Heater at 28C before imaging Imaging da puck in Processing Clamp while storing it to tube 1 @13:22:59.26 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @13:23:16.11 Lights, camera, ACTION! Simulating PuckCamera: snap -bin=2,2 -auto /var/log/esp/da18sep0113h1000ml.tif @13:23:36.11 Waiting >20 seconds for da to relinquish FlushPuck @13:25:06.11 Simulating PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da18sep0113h1000ml15s.tif @13:25:31.11 Simulating PuckCamera: snap -bin=2,2 30.0 /var/log/esp/midres/da18sep0113h1000ml30s.tif @13:26:11.11 Simulating PuckCamera: snap -bin=1,1 70.0 /var/log/esp/hires/da18sep0113h1000ml70s.tif @13:27:32.27 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @13:27:34.61 Loading FlushPuck into Processing Clamp from FlushPuck::Garage Starting finalPurge @13:27:54.61 Waiting >20 seconds for psFlush to relinquish FlushPuck @13:36:52.95 Loading FlushPuck into Collection Clamp from Processing Clamp @13:37:10.79 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @13:37:55.31 Flushing lower manifold's waste1 port @13:38:19.36 Flushing lower manifold's waste2 port @13:38:43.41 Disinfecting external Intake with kill @13:40:16.90 Garaging FlushPuck in Collection Clamp @13:40:30.00 Completed [/home/esp/esp2/mission/2018_08_31_niagara_mission.rb:53] begun 11:30:08 Preparing to sleep until 7:02AM 9/03/2018 Asleep til 7:02AM 9/03/2018 Continuing at 07:02EDT03-Sep-18 @13:40:30.30 Shutdown @07:02:08.26EDT03-Sep-18 Phase [Sample.deep; initialPurge; da withoutWCR] @07:02:09.76 Starting initialPurge Loading FlushPuck into Collection Clamp from FlushPuck::Garage @07:02:25.96 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @07:03:11.60 Purging external Intake @07:03:35.62 Flushing upper manifold @07:04:52.42 Garaging FlushPuck in Collection Clamp @07:05:05.52 da sampling at most 1000ml Loading sh1 puck from tube 2 into Collection Clamp @07:05:20.72 collectSample 1000ml w/bubble point of 24psi Priming 7ml @07:05:20.79 Calibrating pressure and temperature sensors @07:05:34.18 Waiting for external sampler to finish priming... @07:08:34.81 Sampling 1000.0ml @07:33:54.33 Sampled 1000.0ml Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @07:34:41.90 BubblePoint: Intake @14.7psia, Outlet @14.7psia @07:35:02.21 Evacuated: Intake @14.7psia, Outlet @14.7psia @07:35:30.27 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @07:36:16.34 BubblePoint: Intake @14.7psia, Outlet @14.7psia @07:36:36.66 Evacuated: Intake @14.7psia, Outlet @14.7psia @07:37:04.71 Lysing: Adding 2.0ml AqMeOH @07:38:12.54 Heating sh1 puck from tube 2 in Collection Clamp to 70C for 10:00 @07:49:38.54 Collection Puck Heater at 70C after lysing @07:50:19.90 Recovering Lysate into Collection syringe (recycling once) @07:51:01.08 Recycling Lysate (#1 of 1) @07:52:09.30 Recovering Lysate (#2 of 2) @07:52:50.48 Lysing Complete Storing sh1 puck in Collection Clamp to tube 1 @07:52:58.66 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @07:53:12.77 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @07:53:15.11 Tranferring DA Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @07:56:52.19 Garaging FlushPuck in Collection Clamp @07:57:05.29 Loading lyfil puck from tube 2 into Collection Clamp @07:59:34.61 Storing lyfil puck in Collection Clamp to tube 1 @07:59:42.70 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @07:59:56.81 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:00:59.04 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @08:02:17.60 Garaging FlushPuck in Processing Clamp @08:02:29.45 Loading da puck from tube 2 into Processing Clamp @08:02:49.47 Heating da puck from tube 2 in Processing Clamp to 28C for 2:00:00 @08:10:28.99 recoverPSRreagent08 @08:12:16.20 addPMLReagent08 @08:12:58.27 Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:16:34.83 Garaging FlushPuck in Collection Clamp @08:20:58.27 recoverPRVreagent08 @08:21:58.53 washCycleDA #1 of 2 @08:24:54.17 recoverPSRreagent08 @08:26:04.56 washCycleDA #2 of 2 @08:28:59.46 recoverPSRreagent08 @08:30:09.85 getPMLReagent08 @08:30:32.08 addPMLReagent08 @08:39:13.65 recoverPRVreagent08 @08:40:13.16 washCycleDA #1 of 3 @08:43:08.81 recoverPSRreagent08 @08:44:19.20 washCycleDA #2 of 3 @08:47:14.09 recoverPSRreagent08 @08:48:24.48 washCycleDA #3 of 3 @08:51:19.37 recoverPSRreagent08 @08:52:29.76 Mixing DA Substrates @08:53:40.50 recoverPSRreagent08 @08:54:50.89 Processing Puck Heater at 28C before imaging Imaging da puck in Processing Clamp while storing it to tube 1 @08:54:59.12 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:55:15.97 Lights, camera, ACTION! Simulating PuckCamera: snap -bin=2,2 -auto /var/log/esp/da18sep0308h1000ml.tif @08:55:35.97 Waiting >20 seconds for da to relinquish FlushPuck @08:57:05.97 Simulating PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da18sep0308h1000ml15s.tif @08:57:30.97 Simulating PuckCamera: snap -bin=2,2 30.0 /var/log/esp/midres/da18sep0308h1000ml30s.tif @08:58:10.97 Simulating PuckCamera: snap -bin=1,1 70.0 /var/log/esp/hires/da18sep0308h1000ml70s.tif @08:59:32.13 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:59:34.47 Starting finalPurge Loading FlushPuck into Processing Clamp from FlushPuck::Garage @08:59:54.47 Waiting >20 seconds for psFlush to relinquish FlushPuck @09:08:52.81 Loading FlushPuck into Collection Clamp from Processing Clamp @09:09:10.65 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @09:09:55.17 Flushing lower manifold's waste1 port @09:10:19.22 Flushing lower manifold's waste2 port @09:10:43.27 Disinfecting external Intake with kill @09:12:16.76 Garaging FlushPuck in Collection Clamp @09:12:29.86 Completed [Sample.deep; initialPurge; da withoutWCR] begun 07:02:08 Preparing to sleep until 11:30AM 9/03/2018 Asleep til 11:30AM 9/03/2018 Continuing at 11:30 @09:12:30.16 Shutdown @11:30:08.26 Phase [/home/esp/esp2/mission/2018_08_31_niagara_mission.rb:62] @11:30:09.76 Starting initialPurge Loading FlushPuck into Collection Clamp from FlushPuck::Garage @11:30:25.96 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @11:31:11.60 Purging external Intake @11:31:35.62 Flushing upper manifold @11:32:52.42 Garaging FlushPuck in Collection Clamp @11:33:05.52 da sampling at most 1000ml Loading sh1 puck from tube 2 into Collection Clamp @11:33:21.02 collectSample 1000ml w/bubble point of 24psi Priming 7ml @11:33:21.09 Calibrating pressure and temperature sensors @11:33:34.47 Waiting for external sampler to finish priming... @11:36:35.11 Sampling 1000.0ml @12:01:54.63 Sampled 1000.0ml Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @12:02:42.19 BubblePoint: Intake @14.7psia, Outlet @14.7psia @12:03:02.51 Evacuated: Intake @14.7psia, Outlet @14.7psia @12:03:30.56 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @12:04:16.64 BubblePoint: Intake @14.7psia, Outlet @14.7psia @12:04:36.95 Evacuated: Intake @14.7psia, Outlet @14.7psia @12:05:05.01 Lysing: Adding 2.0ml AqMeOH @12:06:12.83 Heating sh1 puck from tube 2 in Collection Clamp to 70C for 10:00 @12:17:38.83 Collection Puck Heater at 70C after lysing @12:18:20.20 Recovering Lysate into Collection syringe (recycling once) @12:19:01.38 Recycling Lysate (#1 of 1) @12:20:09.60 Recovering Lysate (#2 of 2) @12:20:50.78 Lysing Complete Storing sh1 puck in Collection Clamp to tube 1 @12:20:58.66 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:21:12.77 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:21:15.11 Tranferring DA Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:24:52.19 Garaging FlushPuck in Collection Clamp @12:25:05.29 Loading lyfil puck from tube 2 into Collection Clamp @12:27:34.91 Storing lyfil puck in Collection Clamp to tube 1 @12:27:42.70 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:27:56.81 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:28:59.04 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @12:30:17.60 Garaging FlushPuck in Processing Clamp @12:30:29.45 Loading da puck from tube 2 into Processing Clamp @12:30:49.76 Heating da puck from tube 2 in Processing Clamp to 28C for 2:00:00 @12:38:29.29 recoverPSRreagent08 @12:40:16.49 addPMLReagent08 @12:40:58.56 Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:44:35.13 Garaging FlushPuck in Collection Clamp @12:48:58.56 recoverPRVreagent08 @12:49:58.83 washCycleDA #1 of 2 @12:52:54.47 recoverPSRreagent08 @12:54:04.86 washCycleDA #2 of 2 @12:56:59.75 recoverPSRreagent08 @12:58:10.14 getPMLReagent08 @12:58:32.38 addPMLReagent08 @13:07:13.95 recoverPRVreagent08 @13:08:13.46 washCycleDA #1 of 3 @13:11:09.10 recoverPSRreagent08 @13:12:19.49 washCycleDA #2 of 3 @13:15:14.39 recoverPSRreagent08 @13:16:24.78 washCycleDA #3 of 3 @13:19:19.67 recoverPSRreagent08 @13:20:30.06 Mixing DA Substrates @13:21:40.80 recoverPSRreagent08 @13:22:51.19 Processing Puck Heater at 28C before imaging Imaging da puck in Processing Clamp while storing it to tube 1 @13:22:59.12 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @13:23:15.97 Lights, camera, ACTION! Simulating PuckCamera: snap -bin=2,2 -auto /var/log/esp/da18sep0313h1000ml.tif @13:23:35.97 Waiting >20 seconds for da to relinquish FlushPuck @13:25:05.97 Simulating PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da18sep0313h1000ml15s.tif @13:25:30.97 Simulating PuckCamera: snap -bin=2,2 30.0 /var/log/esp/midres/da18sep0313h1000ml30s.tif @13:26:10.97 Simulating PuckCamera: snap -bin=1,1 70.0 /var/log/esp/hires/da18sep0313h1000ml70s.tif @13:27:32.13 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @13:27:34.47 Starting finalPurge Loading FlushPuck into Processing Clamp from FlushPuck::Garage @13:27:54.47 Waiting >20 seconds for psFlush to relinquish FlushPuck @13:36:52.81 Loading FlushPuck into Collection Clamp from Processing Clamp @13:37:10.65 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @13:37:55.17 Flushing lower manifold's waste1 port @13:38:19.22 Flushing lower manifold's waste2 port @13:38:43.27 Disinfecting external Intake with kill @13:40:16.76 Garaging FlushPuck in Collection Clamp @13:40:29.86 Completed [/home/esp/esp2/mission/2018_08_31_niagara_mission.rb:62] begun 11:30:08 Preparing to sleep until 7:04AM 9/05/2018 Asleep til 7:04AM 9/05/2018 Continuing at 07:04EDT05-Sep-18 @13:40:30.16 Shutdown @07:04:08.26EDT05-Sep-18 Phase [Sample.deep; initialPurge; da withoutWCR] @07:04:09.76 Starting initialPurge Loading FlushPuck into Collection Clamp from FlushPuck::Garage @07:04:25.96 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @07:05:11.60 Purging external Intake @07:05:35.62 Flushing upper manifold @07:06:52.42 Garaging FlushPuck in Collection Clamp @07:07:05.52 da sampling at most 1000ml Loading sh1 puck from tube 2 into Collection Clamp @07:07:21.32 collectSample 1000ml w/bubble point of 24psi Priming 7ml @07:07:21.38 Calibrating pressure and temperature sensors @07:07:34.77 Waiting for external sampler to finish priming... @07:10:35.41 Sampling 1000.0ml @07:35:54.93 Sampled 1000.0ml Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @07:36:42.49 BubblePoint: Intake @14.7psia, Outlet @14.7psia @07:37:02.81 Evacuated: Intake @14.7psia, Outlet @14.7psia @07:37:30.86 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @07:38:16.93 BubblePoint: Intake @14.7psia, Outlet @14.7psia @07:38:37.25 Evacuated: Intake @14.7psia, Outlet @14.7psia @07:39:05.30 Lysing: Adding 2.0ml AqMeOH @07:40:13.13 Heating sh1 puck from tube 2 in Collection Clamp to 70C for 10:00 @07:51:39.13 Collection Puck Heater at 70C after lysing @07:52:20.49 Recovering Lysate into Collection syringe (recycling once) @07:53:01.67 Recycling Lysate (#1 of 1) @07:54:09.90 Recovering Lysate (#2 of 2) @07:54:51.08 Lysing Complete Storing sh1 puck in Collection Clamp to tube 1 @07:54:58.66 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @07:55:12.77 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @07:55:15.11 Tranferring DA Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @07:58:52.19 Garaging FlushPuck in Collection Clamp @07:59:05.29 Loading lyfil puck from tube 2 into Collection Clamp @08:01:35.21 Storing lyfil puck in Collection Clamp to tube 1 @08:01:42.70 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:01:56.81 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:02:59.04 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @08:04:17.60 Garaging FlushPuck in Processing Clamp @08:04:29.45 Loading da puck from tube 2 into Processing Clamp @08:04:50.06 Heating da puck from tube 2 in Processing Clamp to 28C for 2:00:00 @08:12:29.59 recoverPSRreagent08 @08:14:16.79 addPMLReagent08 @08:14:58.86 Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:18:35.43 Garaging FlushPuck in Collection Clamp @08:22:58.86 recoverPRVreagent08 @08:23:59.12 washCycleDA #1 of 2 @08:26:54.77 recoverPSRreagent08 @08:28:05.16 washCycleDA #2 of 2 @08:31:00.05 recoverPSRreagent08 @08:32:10.44 getPMLReagent08 @08:32:32.68 addPMLReagent08 @08:41:14.25 recoverPRVreagent08 @08:42:13.76 washCycleDA #1 of 3 @08:45:09.40 recoverPSRreagent08 @08:46:19.79 washCycleDA #2 of 3 @08:49:14.68 recoverPSRreagent08 @08:50:25.07 washCycleDA #3 of 3 @08:53:19.97 recoverPSRreagent08 @08:54:30.36 Mixing DA Substrates @08:55:41.10 recoverPSRreagent08 @08:56:51.49 Processing Puck Heater at 28C before imaging Imaging da puck in Processing Clamp while storing it to tube 1 @08:56:59.12 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:57:15.97 Lights, camera, ACTION! Simulating PuckCamera: snap -bin=2,2 -auto /var/log/esp/da18sep0508h1000ml.tif @08:57:35.97 Waiting >20 seconds for da to relinquish FlushPuck @08:59:05.97 Simulating PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da18sep0508h1000ml15s.tif @08:59:30.97 Simulating PuckCamera: snap -bin=2,2 30.0 /var/log/esp/midres/da18sep0508h1000ml30s.tif @09:00:10.97 Simulating PuckCamera: snap -bin=1,1 70.0 /var/log/esp/hires/da18sep0508h1000ml70s.tif @09:01:32.13 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @09:01:34.47 Loading FlushPuck into Processing Clamp from FlushPuck::Garage Starting finalPurge @09:01:54.47 Waiting >20 seconds for psFlush to relinquish FlushPuck @09:10:52.81 Loading FlushPuck into Collection Clamp from Processing Clamp @09:11:10.65 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @09:11:55.17 Flushing lower manifold's waste1 port @09:12:19.22 Flushing lower manifold's waste2 port @09:12:43.27 Disinfecting external Intake with kill @09:14:16.76 Garaging FlushPuck in Collection Clamp @09:14:29.86 Completed [Sample.deep; initialPurge; da withoutWCR] begun 07:04:08 Preparing to sleep until 11:30AM 9/05/2018 Asleep til 11:30AM 9/05/2018 Continuing at 11:30 @09:14:30.16 Shutdown @11:30:08.26 Phase [/home/esp/esp2/mission/2018_08_31_niagara_mission.rb:71] @11:30:09.76 Starting initialPurge Loading FlushPuck into Collection Clamp from FlushPuck::Garage @11:30:25.96 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @11:31:11.60 Purging external Intake @11:31:35.62 Flushing upper manifold @11:32:52.42 Garaging FlushPuck in Collection Clamp @11:33:05.52 da sampling at most 1000ml Loading sh1 puck from tube 2 into Collection Clamp @11:33:21.61 collectSample 1000ml w/bubble point of 24psi Priming 7ml @11:33:21.68 Calibrating pressure and temperature sensors @11:33:35.07 Waiting for external sampler to finish priming... @11:36:35.70 Sampling 1000.0ml @12:01:55.22 Sampled 1000.0ml Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @12:02:42.79 BubblePoint: Intake @14.7psia, Outlet @14.7psia @12:03:03.10 Evacuated: Intake @14.7psia, Outlet @14.7psia @12:03:31.16 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @12:04:17.23 BubblePoint: Intake @14.7psia, Outlet @14.7psia @12:04:37.55 Evacuated: Intake @14.7psia, Outlet @14.7psia @12:05:05.60 Lysing: Adding 2.0ml AqMeOH @12:06:13.43 Heating sh1 puck from tube 2 in Collection Clamp to 70C for 10:00 @12:17:39.43 Collection Puck Heater at 70C after lysing @12:18:20.79 Recovering Lysate into Collection syringe (recycling once) @12:19:01.97 Recycling Lysate (#1 of 1) @12:20:10.19 Recovering Lysate (#2 of 2) @12:20:51.37 Lysing Complete Storing sh1 puck in Collection Clamp to tube 1 @12:20:58.66 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:21:12.77 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:21:15.11 Tranferring DA Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:24:52.19 Garaging FlushPuck in Collection Clamp @12:25:05.29 Loading lyfil puck from tube 2 into Collection Clamp @12:27:35.50 Storing lyfil puck in Collection Clamp to tube 1 @12:27:42.70 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:27:56.81 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:28:59.04 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @12:30:17.60 Garaging FlushPuck in Processing Clamp @12:30:29.45 Loading da puck from tube 2 into Processing Clamp @12:30:50.34 Heating da puck from tube 2 in Processing Clamp to 28C for 2:00:00 @12:38:29.87 recoverPSRreagent08 @12:40:17.07 addPMLReagent08 @12:40:59.14 Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:44:35.71 Garaging FlushPuck in Collection Clamp @12:48:59.14 recoverPRVreagent08 @12:49:59.40 washCycleDA #1 of 2 @12:52:55.05 recoverPSRreagent08 @12:54:05.44 washCycleDA #2 of 2 @12:57:00.33 recoverPSRreagent08 @12:58:10.72 getPMLReagent08 @12:58:32.96 addPMLReagent08 @13:07:14.53 recoverPRVreagent08 @13:08:14.04 washCycleDA #1 of 3 @13:11:09.68 recoverPSRreagent08 @13:12:20.07 washCycleDA #2 of 3 @13:15:14.97 recoverPSRreagent08 @13:16:25.36 washCycleDA #3 of 3 @13:19:20.25 recoverPSRreagent08 @13:20:30.64 Mixing DA Substrates @13:21:41.38 recoverPSRreagent08 @13:22:51.77 Processing Puck Heater at 28C before imaging Imaging da puck in Processing Clamp while storing it to tube 1 @13:22:59.10 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @13:23:15.95 Lights, camera, ACTION! Simulating PuckCamera: snap -bin=2,2 -auto /var/log/esp/da18sep0513h1000ml.tif @13:23:35.95 Waiting >20 seconds for da to relinquish FlushPuck @13:25:05.95 Simulating PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da18sep0513h1000ml15s.tif @13:25:30.95 Simulating PuckCamera: snap -bin=2,2 30.0 /var/log/esp/midres/da18sep0513h1000ml30s.tif @13:26:10.95 Simulating PuckCamera: snap -bin=1,1 70.0 /var/log/esp/hires/da18sep0513h1000ml70s.tif @13:27:32.11 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @13:27:34.45 Starting finalPurge Loading FlushPuck into Processing Clamp from FlushPuck::Garage @13:27:54.45 Waiting >20 seconds for psFlush to relinquish FlushPuck @13:36:52.79 Loading FlushPuck into Collection Clamp from Processing Clamp @13:37:10.64 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @13:37:55.16 Flushing lower manifold's waste1 port @13:38:19.21 Flushing lower manifold's waste2 port @13:38:43.26 Disinfecting external Intake with kill @13:40:16.74 Garaging FlushPuck in Collection Clamp @13:40:29.84 Completed [/home/esp/esp2/mission/2018_08_31_niagara_mission.rb:71] begun 11:30:08 Preparing to sleep until 7:06AM 9/07/2018 Asleep til 7:06AM 9/07/2018 Continuing at 07:06EDT07-Sep-18 @13:40:30.14 Shutdown @07:06:08.26EDT07-Sep-18 Phase [Sample.deep; initialPurge; da withoutWCR] @07:06:09.76 Starting initialPurge Loading FlushPuck into Collection Clamp from FlushPuck::Garage @07:06:25.96 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @07:07:11.60 Purging external Intake @07:07:35.62 Flushing upper manifold @07:08:52.42 Garaging FlushPuck in Collection Clamp @07:09:05.52 da sampling at most 1000ml Loading sh1 puck from tube 2 into Collection Clamp @07:09:21.91 collectSample 1000ml w/bubble point of 24psi Priming 7ml @07:09:21.98 Calibrating pressure and temperature sensors @07:09:35.37 Waiting for external sampler to finish priming... @07:12:36.00 Sampling 1000.0ml @07:37:55.52 Sampled 1000.0ml Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @07:38:43.08 BubblePoint: Intake @14.7psia, Outlet @14.7psia @07:39:03.40 Evacuated: Intake @14.7psia, Outlet @14.7psia @07:39:31.46 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @07:40:17.53 BubblePoint: Intake @14.7psia, Outlet @14.7psia @07:40:37.84 Evacuated: Intake @14.7psia, Outlet @14.7psia @07:41:05.90 Lysing: Adding 2.0ml AqMeOH @07:42:13.72 Heating sh1 puck from tube 2 in Collection Clamp to 70C for 10:00 @07:53:39.72 Collection Puck Heater at 70C after lysing @07:54:21.09 Recovering Lysate into Collection syringe (recycling once) @07:55:02.27 Recycling Lysate (#1 of 1) @07:56:10.49 Recovering Lysate (#2 of 2) @07:56:51.67 Lysing Complete Storing sh1 puck in Collection Clamp to tube 1 @07:56:58.66 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @07:57:12.77 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @07:57:15.11 Tranferring DA Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:00:52.19 Garaging FlushPuck in Collection Clamp @08:01:05.29 Loading lyfil puck from tube 3 into Collection Clamp @08:03:36.75 Storing lyfil puck in Collection Clamp to tube 2 @08:03:51.22 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:04:54.26 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @08:06:12.82 Garaging FlushPuck in Processing Clamp @08:06:24.67 Loading da puck from tube 3 into Processing Clamp @08:06:43.67 Heating da puck from tube 3 in Processing Clamp to 28C for 2:00:00 @08:14:23.20 recoverPSRreagent08 @08:16:10.40 addPMLReagent08 @08:16:52.47 Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:20:29.04 Garaging FlushPuck in Collection Clamp @08:24:52.47 recoverPRVreagent08 @08:25:52.73 washCycleDA #1 of 2 @08:28:48.38 recoverPSRreagent08 @08:29:58.77 washCycleDA #2 of 2 @08:32:53.66 recoverPSRreagent08 @08:34:04.05 getPMLReagent08 @08:34:26.29 addPMLReagent08 @08:43:07.86 recoverPRVreagent08 @08:44:07.37 washCycleDA #1 of 3 @08:47:03.01 recoverPSRreagent08 @08:48:13.40 washCycleDA #2 of 3 @08:51:08.29 recoverPSRreagent08 @08:52:18.68 washCycleDA #3 of 3 @08:55:13.58 recoverPSRreagent08 @08:56:23.97 Mixing DA Substrates @08:57:34.71 recoverPSRreagent08 @08:58:45.10 Processing Puck Heater at 28C before imaging Imaging da puck in Processing Clamp while storing it to tube 2 @08:58:54.29 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:59:11.14 Lights, camera, ACTION! Simulating PuckCamera: snap -bin=2,2 -auto /var/log/esp/da18sep0708h1000ml.tif @08:59:31.14 Waiting >20 seconds for da to relinquish FlushPuck @09:01:01.14 Simulating PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da18sep0708h1000ml15s.tif @09:01:26.14 Simulating PuckCamera: snap -bin=2,2 30.0 /var/log/esp/midres/da18sep0708h1000ml30s.tif @09:02:06.14 Simulating PuckCamera: snap -bin=1,1 70.0 /var/log/esp/hires/da18sep0708h1000ml70s.tif @09:03:27.30 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @09:03:30.25 Starting finalPurge Loading FlushPuck into Processing Clamp from FlushPuck::Garage @09:03:50.25 Waiting >20 seconds for psFlush to relinquish FlushPuck @09:12:48.59 Loading FlushPuck into Collection Clamp from Processing Clamp @09:13:06.43 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @09:13:50.95 Flushing lower manifold's waste1 port @09:14:15.00 Flushing lower manifold's waste2 port @09:14:39.05 Disinfecting external Intake with kill @09:16:12.54 Garaging FlushPuck in Collection Clamp @09:16:25.64 Completed [Sample.deep; initialPurge; da withoutWCR] begun 07:06:08 Preparing to sleep until 11:30AM 9/07/2018 Asleep til 11:30AM 9/07/2018 Continuing at 11:30 @09:16:25.94 Shutdown @11:30:04.90 Phase [/home/esp/esp2/mission/2018_08_31_niagara_mission.rb:80] @11:30:06.40 Starting initialPurge Loading FlushPuck into Collection Clamp from FlushPuck::Garage @11:30:22.60 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @11:31:08.24 Purging external Intake @11:31:32.26 Flushing upper manifold @11:32:49.06 Garaging FlushPuck in Collection Clamp @11:33:02.16 da sampling at most 1000ml Loading sh1 puck from tube 3 into Collection Clamp @11:33:20.09 collectSample 1000ml w/bubble point of 24psi Priming 7ml @11:33:20.15 Calibrating pressure and temperature sensors @11:33:33.54 Waiting for external sampler to finish priming... @11:36:34.18 Sampling 1000.0ml @12:01:53.70 Sampled 1000.0ml Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @12:02:41.26 BubblePoint: Intake @14.7psia, Outlet @14.7psia @12:03:01.58 Evacuated: Intake @14.7psia, Outlet @14.7psia @12:03:29.63 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @12:04:15.70 BubblePoint: Intake @14.7psia, Outlet @14.7psia @12:04:36.02 Evacuated: Intake @14.7psia, Outlet @14.7psia @12:05:04.07 Lysing: Adding 2.0ml AqMeOH @12:06:11.90 Heating sh1 puck from tube 3 in Collection Clamp to 70C for 10:00 @12:17:37.90 Collection Puck Heater at 70C after lysing @12:18:19.26 Recovering Lysate into Collection syringe (recycling once) @12:19:00.44 Recycling Lysate (#1 of 1) @12:20:08.67 Recovering Lysate (#2 of 2) @12:20:49.84 Lysing Complete Storing sh1 puck in Collection Clamp to tube 2 @12:20:58.69 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:21:12.81 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:21:15.66 Tranferring DA Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:24:52.74 Garaging FlushPuck in Collection Clamp @12:25:05.84 Loading lyfil puck from tube 3 into Collection Clamp @12:27:34.46 Storing lyfil puck in Collection Clamp to tube 2 @12:27:43.22 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:27:57.33 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:28:59.98 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @12:30:18.54 Garaging FlushPuck in Processing Clamp @12:30:30.39 Loading da puck from tube 3 into Processing Clamp @12:30:49.69 Heating da puck from tube 3 in Processing Clamp to 28C for 2:00:00 @12:38:29.22 recoverPSRreagent08 @12:40:16.42 addPMLReagent08 @12:40:58.49 Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:44:35.05 Garaging FlushPuck in Collection Clamp @12:48:58.49 recoverPRVreagent08 @12:49:58.75 washCycleDA #1 of 2 @12:52:54.40 recoverPSRreagent08 @12:54:04.79 washCycleDA #2 of 2 @12:56:59.68 recoverPSRreagent08 @12:58:10.07 getPMLReagent08 @12:58:32.30 addPMLReagent08 @13:07:13.88 recoverPRVreagent08 @13:08:13.38 washCycleDA #1 of 3 @13:11:09.03 recoverPSRreagent08 @13:12:19.42 washCycleDA #2 of 3 @13:15:14.31 recoverPSRreagent08 @13:16:24.70 washCycleDA #3 of 3 @13:19:19.59 recoverPSRreagent08 @13:20:29.98 Mixing DA Substrates @13:21:40.73 recoverPSRreagent08 @13:22:51.12 Processing Puck Heater at 28C before imaging Imaging da puck in Processing Clamp while storing it to tube 2 @13:23:00.01 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @13:23:16.86 Lights, camera, ACTION! Simulating PuckCamera: snap -bin=2,2 -auto /var/log/esp/da18sep0713h1000ml.tif @13:23:36.86 Waiting >20 seconds for da to relinquish FlushPuck @13:25:06.86 Simulating PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da18sep0713h1000ml15s.tif @13:25:31.86 Simulating PuckCamera: snap -bin=2,2 30.0 /var/log/esp/midres/da18sep0713h1000ml30s.tif @13:26:11.86 Simulating PuckCamera: snap -bin=1,1 70.0 /var/log/esp/hires/da18sep0713h1000ml70s.tif @13:27:33.02 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @13:27:35.67 Loading FlushPuck into Processing Clamp from FlushPuck::Garage Starting finalPurge @13:27:55.67 Waiting >20 seconds for psFlush to relinquish FlushPuck @13:36:54.02 Loading FlushPuck into Collection Clamp from Processing Clamp @13:37:11.86 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @13:37:56.38 Flushing lower manifold's waste1 port @13:38:20.43 Flushing lower manifold's waste2 port @13:38:44.48 Disinfecting external Intake with kill @13:40:17.97 Garaging FlushPuck in Collection Clamp @13:40:31.07 Completed [/home/esp/esp2/mission/2018_08_31_niagara_mission.rb:80] begun 11:30:04 Preparing to sleep until 7:08AM 9/09/2018 Asleep til 7:08AM 9/09/2018 Continuing at 07:08EDT09-Sep-18 @13:40:31.37 Shutdown @07:08:04.90EDT09-Sep-18 Phase [Sample.deep; initialPurge; da withoutWCR] @07:08:06.40 Starting initialPurge Loading FlushPuck into Collection Clamp from FlushPuck::Garage @07:08:22.60 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @07:09:08.24 Purging external Intake @07:09:32.26 Flushing upper manifold @07:10:49.06 Garaging FlushPuck in Collection Clamp @07:11:02.16 da sampling at most 1000ml Loading sh1 puck from tube 3 into Collection Clamp @07:11:20.38 collectSample 1000ml w/bubble point of 24psi Priming 7ml @07:11:20.45 Calibrating pressure and temperature sensors @07:11:33.84 Waiting for external sampler to finish priming... @07:14:34.47 Sampling 1000.0ml @07:39:53.99 Sampled 1000.0ml Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @07:40:41.56 BubblePoint: Intake @14.7psia, Outlet @14.7psia @07:41:01.87 Evacuated: Intake @14.7psia, Outlet @14.7psia @07:41:29.93 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @07:42:16.00 BubblePoint: Intake @14.7psia, Outlet @14.7psia @07:42:36.32 Evacuated: Intake @14.7psia, Outlet @14.7psia @07:43:04.37 Lysing: Adding 2.0ml AqMeOH @07:44:12.19 Heating sh1 puck from tube 3 in Collection Clamp to 70C for 10:00 @07:55:38.19 Collection Puck Heater at 70C after lysing @07:56:19.56 Recovering Lysate into Collection syringe (recycling once) @07:57:00.74 Recycling Lysate (#1 of 1) @07:58:08.96 Recovering Lysate (#2 of 2) @07:58:50.14 Lysing Complete Storing sh1 puck in Collection Clamp to tube 2 @07:58:58.71 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @07:59:12.82 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @07:59:15.38 Tranferring DA Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:02:52.46 Garaging FlushPuck in Collection Clamp @08:03:05.56 Loading lyfil puck from tube 3 into Collection Clamp @08:05:34.48 Storing lyfil puck in Collection Clamp to tube 2 @08:05:42.93 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:05:57.05 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:06:59.40 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @08:08:17.96 Garaging FlushPuck in Processing Clamp @08:08:29.81 Loading da puck from tube 3 into Processing Clamp @08:08:49.41 Heating da puck from tube 3 in Processing Clamp to 28C for 2:00:00 @08:16:28.94 recoverPSRreagent08 @08:18:16.14 addPMLReagent08 @08:18:58.21 Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:22:34.77 Garaging FlushPuck in Collection Clamp @08:26:58.21 recoverPRVreagent08 @08:27:58.47 washCycleDA #1 of 2 @08:30:54.11 recoverPSRreagent08 @08:32:04.50 washCycleDA #2 of 2 @08:34:59.40 recoverPSRreagent08 @08:36:09.79 getPMLReagent08 @08:36:32.02 addPMLReagent08 @08:45:13.60 recoverPRVreagent08 @08:46:13.10 washCycleDA #1 of 3 @08:49:08.75 recoverPSRreagent08 @08:50:19.14 washCycleDA #2 of 3 @08:53:14.03 recoverPSRreagent08 @08:54:24.42 washCycleDA #3 of 3 @08:57:19.31 recoverPSRreagent08 @08:58:29.70 Mixing DA Substrates @08:59:40.44 recoverPSRreagent08 @09:00:50.83 Processing Puck Heater at 28C before imaging Imaging da puck in Processing Clamp while storing it to tube 2 @09:00:59.43 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @09:01:16.28 Lights, camera, ACTION! Simulating PuckCamera: snap -bin=2,2 -auto /var/log/esp/da18sep0909h1000ml.tif @09:01:36.28 Waiting >20 seconds for da to relinquish FlushPuck @09:03:06.28 Simulating PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da18sep0909h1000ml15s.tif @09:03:31.28 Simulating PuckCamera: snap -bin=2,2 30.0 /var/log/esp/midres/da18sep0909h1000ml30s.tif @09:04:11.28 Simulating PuckCamera: snap -bin=1,1 70.0 /var/log/esp/hires/da18sep0909h1000ml70s.tif @09:05:32.44 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @09:05:34.80 Starting finalPurge Loading FlushPuck into Processing Clamp from FlushPuck::Garage @09:05:54.80 Waiting >20 seconds for psFlush to relinquish FlushPuck @09:14:53.14 Loading FlushPuck into Collection Clamp from Processing Clamp @09:15:10.98 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @09:15:55.50 Flushing lower manifold's waste1 port @09:16:19.55 Flushing lower manifold's waste2 port @09:16:43.60 Disinfecting external Intake with kill @09:18:17.09 Garaging FlushPuck in Collection Clamp @09:18:30.19 Completed [Sample.deep; initialPurge; da withoutWCR] begun 07:08:04 Preparing to sleep until 11:30AM 9/09/2018 Asleep til 11:30AM 9/09/2018 Continuing at 11:30 @09:18:30.49 Shutdown @11:30:04.90 Phase [/home/esp/esp2/mission/2018_08_31_niagara_mission.rb:89] @11:30:06.40 Starting initialPurge Loading FlushPuck into Collection Clamp from FlushPuck::Garage @11:30:22.60 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @11:31:08.24 Purging external Intake @11:31:32.26 Flushing upper manifold @11:32:49.06 Garaging FlushPuck in Collection Clamp @11:33:02.16 da sampling at most 1000ml Loading sh1 puck from tube 3 into Collection Clamp @11:33:20.68 collectSample 1000ml w/bubble point of 24psi Priming 7ml @11:33:20.74 Calibrating pressure and temperature sensors @11:33:34.13 Waiting for external sampler to finish priming... @11:36:34.77 Sampling 1000.0ml @12:01:54.29 Sampled 1000.0ml Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @12:02:41.85 BubblePoint: Intake @14.7psia, Outlet @14.7psia @12:03:02.17 Evacuated: Intake @14.7psia, Outlet @14.7psia @12:03:30.22 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @12:04:16.30 BubblePoint: Intake @14.7psia, Outlet @14.7psia @12:04:36.61 Evacuated: Intake @14.7psia, Outlet @14.7psia @12:05:04.67 Lysing: Adding 2.0ml AqMeOH @12:06:12.49 Heating sh1 puck from tube 3 in Collection Clamp to 70C for 10:00 @12:17:38.49 Collection Puck Heater at 70C after lysing @12:18:19.86 Recovering Lysate into Collection syringe (recycling once) @12:19:01.04 Recycling Lysate (#1 of 1) @12:20:09.26 Recovering Lysate (#2 of 2) @12:20:50.44 Lysing Complete Storing sh1 puck in Collection Clamp to tube 2 @12:20:58.71 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:21:12.82 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:21:15.16 Tranferring DA Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:24:52.24 Garaging FlushPuck in Collection Clamp @12:25:05.34 Loading lyfil puck from tube 3 into Collection Clamp @12:27:34.55 Storing lyfil puck in Collection Clamp to tube 2 @12:27:42.72 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:27:56.83 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:28:59.06 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @12:30:17.62 Garaging FlushPuck in Processing Clamp @12:30:29.47 Loading da puck from tube 3 into Processing Clamp @12:30:49.36 Heating da puck from tube 3 in Processing Clamp to 28C for 2:00:00 @12:38:28.89 recoverPSRreagent08 @12:40:16.09 addPMLReagent08 @12:40:58.16 Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:44:34.73 Garaging FlushPuck in Collection Clamp @12:48:58.16 recoverPRVreagent08 @12:49:58.42 washCycleDA #1 of 2 @12:52:54.07 recoverPSRreagent08 @12:54:04.46 washCycleDA #2 of 2 @12:56:59.35 recoverPSRreagent08 @12:58:09.74 getPMLReagent08 @12:58:31.98 addPMLReagent08 @13:07:13.55 recoverPRVreagent08 @13:08:13.06 washCycleDA #1 of 3 @13:11:08.70 recoverPSRreagent08 @13:12:19.09 washCycleDA #2 of 3 @13:15:13.98 recoverPSRreagent08 @13:16:24.37 washCycleDA #3 of 3 @13:19:19.27 recoverPSRreagent08 @13:20:29.66 Mixing DA Substrates @13:21:40.40 recoverPSRreagent08 @13:22:50.79 Processing Puck Heater at 28C before imaging Imaging da puck in Processing Clamp while storing it to tube 2 @13:22:59.09 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @13:23:15.94 Lights, camera, ACTION! Simulating PuckCamera: snap -bin=2,2 -auto /var/log/esp/da18sep0913h1000ml.tif @13:23:35.94 Waiting >20 seconds for da to relinquish FlushPuck @13:25:05.94 Simulating PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da18sep0913h1000ml15s.tif @13:25:30.94 Simulating PuckCamera: snap -bin=2,2 30.0 /var/log/esp/midres/da18sep0913h1000ml30s.tif @13:26:10.94 Simulating PuckCamera: snap -bin=1,1 70.0 /var/log/esp/hires/da18sep0913h1000ml70s.tif @13:27:32.10 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @13:27:34.44 Loading FlushPuck into Processing Clamp from FlushPuck::Garage Starting finalPurge @13:27:54.44 Waiting >20 seconds for psFlush to relinquish FlushPuck @13:36:52.78 Loading FlushPuck into Collection Clamp from Processing Clamp @13:37:10.62 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @13:37:55.14 Flushing lower manifold's waste1 port @13:38:19.19 Flushing lower manifold's waste2 port @13:38:43.24 Disinfecting external Intake with kill @13:40:16.73 Garaging FlushPuck in Collection Clamp @13:40:29.83 Completed [/home/esp/esp2/mission/2018_08_31_niagara_mission.rb:89] begun 11:30:04 Preparing to sleep until 7:10AM 9/11/2018 Asleep til 7:10AM 9/11/2018 Continuing at 07:10EDT11-Sep-18 @13:40:30.13 Shutdown @07:10:04.90EDT11-Sep-18 Phase [Sample.deep; initialPurge; da withoutWCR] @07:10:06.40 Starting initialPurge Loading FlushPuck into Collection Clamp from FlushPuck::Garage @07:10:22.60 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @07:11:08.24 Purging external Intake @07:11:32.26 Flushing upper manifold @07:12:49.06 Garaging FlushPuck in Collection Clamp @07:13:02.16 da sampling at most 1000ml Loading sh1 puck from tube 3 into Collection Clamp @07:13:20.98 collectSample 1000ml w/bubble point of 24psi Priming 7ml @07:13:21.04 Calibrating pressure and temperature sensors @07:13:34.43 Waiting for external sampler to finish priming... @07:16:35.07 Sampling 1000.0ml @07:41:54.59 Sampled 1000.0ml Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @07:42:42.15 BubblePoint: Intake @14.7psia, Outlet @14.7psia @07:43:02.47 Evacuated: Intake @14.7psia, Outlet @14.7psia @07:43:30.52 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @07:44:16.59 BubblePoint: Intake @14.7psia, Outlet @14.7psia @07:44:36.91 Evacuated: Intake @14.7psia, Outlet @14.7psia @07:45:04.96 Lysing: Adding 2.0ml AqMeOH @07:46:12.79 Heating sh1 puck from tube 3 in Collection Clamp to 70C for 10:00 @07:57:38.79 Collection Puck Heater at 70C after lysing @07:58:20.15 Recovering Lysate into Collection syringe (recycling once) @07:59:01.33 Recycling Lysate (#1 of 1) @08:00:09.56 Recovering Lysate (#2 of 2) @08:00:50.74 Lysing Complete Storing sh1 puck in Collection Clamp to tube 2 @08:00:58.71 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:01:12.82 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:01:15.16 Tranferring DA Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:04:52.24 Garaging FlushPuck in Collection Clamp @08:05:05.34 Loading lyfil puck from tube 3 into Collection Clamp @08:07:34.85 Storing lyfil puck in Collection Clamp to tube 2 @08:07:42.72 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:07:56.83 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:08:59.06 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @08:10:17.62 Garaging FlushPuck in Processing Clamp @08:10:29.47 Loading da puck from tube 3 into Processing Clamp @08:10:49.66 Heating da puck from tube 3 in Processing Clamp to 28C for 2:00:00 @08:18:29.19 recoverPSRreagent08 @08:20:16.39 addPMLReagent08 @08:20:58.46 Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:24:35.02 Garaging FlushPuck in Collection Clamp @08:28:58.46 recoverPRVreagent08 @08:29:58.72 washCycleDA #1 of 2 @08:32:54.36 recoverPSRreagent08 @08:34:04.75 washCycleDA #2 of 2 @08:36:59.65 recoverPSRreagent08 @08:38:10.04 getPMLReagent08 @08:38:32.27 addPMLReagent08 @08:47:13.85 recoverPRVreagent08 @08:48:13.35 washCycleDA #1 of 3 @08:51:09.00 recoverPSRreagent08 @08:52:19.39 washCycleDA #2 of 3 @08:55:14.28 recoverPSRreagent08 @08:56:24.67 washCycleDA #3 of 3 @08:59:19.56 recoverPSRreagent08 @09:00:29.95 Mixing DA Substrates @09:01:40.69 recoverPSRreagent08 @09:02:51.08 Processing Puck Heater at 28C before imaging Imaging da puck in Processing Clamp while storing it to tube 2 @09:02:59.09 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @09:03:15.94 Lights, camera, ACTION! Simulating PuckCamera: snap -bin=2,2 -auto /var/log/esp/da18sep1109h1000ml.tif @09:03:35.94 Waiting >20 seconds for da to relinquish FlushPuck @09:05:05.94 Simulating PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da18sep1109h1000ml15s.tif @09:05:30.94 Simulating PuckCamera: snap -bin=2,2 30.0 /var/log/esp/midres/da18sep1109h1000ml30s.tif @09:06:10.94 Simulating PuckCamera: snap -bin=1,1 70.0 /var/log/esp/hires/da18sep1109h1000ml70s.tif @09:07:32.10 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @09:07:34.44 Loading FlushPuck into Processing Clamp from FlushPuck::Garage Starting finalPurge @09:07:54.44 Waiting >20 seconds for psFlush to relinquish FlushPuck @09:16:52.78 Loading FlushPuck into Collection Clamp from Processing Clamp @09:17:10.62 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @09:17:55.14 Flushing lower manifold's waste1 port @09:18:19.19 Flushing lower manifold's waste2 port @09:18:43.24 Disinfecting external Intake with kill @09:20:16.73 Garaging FlushPuck in Collection Clamp @09:20:29.83 Completed [Sample.deep; initialPurge; da withoutWCR] begun 07:10:04 Preparing to sleep until 11:30AM 9/11/2018 Asleep til 11:30AM 9/11/2018 Continuing at 11:30 @09:20:30.13 Shutdown @11:30:04.90 Phase [/home/esp/esp2/mission/2018_08_31_niagara_mission.rb:98] @11:30:06.40 Starting initialPurge Loading FlushPuck into Collection Clamp from FlushPuck::Garage @11:30:22.60 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @11:31:08.24 Purging external Intake @11:31:32.26 Flushing upper manifold @11:32:49.06 Garaging FlushPuck in Collection Clamp @11:33:02.16 da sampling at most 1000ml Loading sh1 puck from tube 3 into Collection Clamp @11:33:21.27 collectSample 1000ml w/bubble point of 24psi Priming 7ml @11:33:21.34 Calibrating pressure and temperature sensors @11:33:34.73 Waiting for external sampler to finish priming... @11:36:35.36 Sampling 1000.0ml @12:01:54.88 Sampled 1000.0ml Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @12:02:42.45 BubblePoint: Intake @14.7psia, Outlet @14.7psia @12:03:02.76 Evacuated: Intake @14.7psia, Outlet @14.7psia @12:03:30.82 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @12:04:16.89 BubblePoint: Intake @14.7psia, Outlet @14.7psia @12:04:37.21 Evacuated: Intake @14.7psia, Outlet @14.7psia @12:05:05.26 Lysing: Adding 2.0ml AqMeOH @12:06:13.09 Heating sh1 puck from tube 3 in Collection Clamp to 70C for 10:00 @12:17:39.09 Collection Puck Heater at 70C after lysing @12:18:20.45 Recovering Lysate into Collection syringe (recycling once) @12:19:01.63 Recycling Lysate (#1 of 1) @12:20:09.85 Recovering Lysate (#2 of 2) @12:20:51.03 Lysing Complete Storing sh1 puck in Collection Clamp to tube 2 @12:20:58.71 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:21:12.82 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:21:15.16 Tranferring DA Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:24:52.24 Garaging FlushPuck in Collection Clamp @12:25:05.34 Loading lyfil puck from tube 3 into Collection Clamp @12:27:35.15 Storing lyfil puck in Collection Clamp to tube 2 @12:27:42.72 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:27:56.83 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:28:59.06 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @12:30:17.62 Garaging FlushPuck in Processing Clamp @12:30:29.47 Loading da puck from tube 3 into Processing Clamp @12:30:49.95 Heating da puck from tube 3 in Processing Clamp to 28C for 2:00:00 @12:38:29.48 recoverPSRreagent08 @12:40:16.68 addPMLReagent08 @12:40:58.75 Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:44:35.32 Garaging FlushPuck in Collection Clamp @12:48:58.75 recoverPRVreagent08 @12:49:59.02 washCycleDA #1 of 2 @12:52:54.66 recoverPSRreagent08 @12:54:05.05 washCycleDA #2 of 2 @12:56:59.94 recoverPSRreagent08 @12:58:10.33 getPMLReagent08 @12:58:32.57 addPMLReagent08 @13:07:14.14 recoverPRVreagent08 @13:08:13.65 washCycleDA #1 of 3 @13:11:09.30 recoverPSRreagent08 @13:12:19.69 washCycleDA #2 of 3 @13:15:14.58 recoverPSRreagent08 @13:16:24.97 washCycleDA #3 of 3 @13:19:19.86 recoverPSRreagent08 @13:20:30.25 Mixing DA Substrates @13:21:40.99 recoverPSRreagent08 @13:22:51.38 Processing Puck Heater at 28C before imaging Imaging da puck in Processing Clamp while storing it to tube 2 @13:22:59.09 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @13:23:15.94 Lights, camera, ACTION! Simulating PuckCamera: snap -bin=2,2 -auto /var/log/esp/da18sep1113h1000ml.tif @13:23:35.94 Waiting >20 seconds for da to relinquish FlushPuck @13:25:05.94 Simulating PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da18sep1113h1000ml15s.tif @13:25:30.94 Simulating PuckCamera: snap -bin=2,2 30.0 /var/log/esp/midres/da18sep1113h1000ml30s.tif @13:26:10.94 Simulating PuckCamera: snap -bin=1,1 70.0 /var/log/esp/hires/da18sep1113h1000ml70s.tif @13:27:32.10 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @13:27:34.44 Starting finalPurge Loading FlushPuck into Processing Clamp from FlushPuck::Garage @13:27:54.44 Waiting >20 seconds for psFlush to relinquish FlushPuck @13:36:52.78 Loading FlushPuck into Collection Clamp from Processing Clamp @13:37:10.62 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @13:37:55.14 Flushing lower manifold's waste1 port @13:38:19.19 Flushing lower manifold's waste2 port @13:38:43.24 Disinfecting external Intake with kill @13:40:16.73 Garaging FlushPuck in Collection Clamp @13:40:29.83 Completed [/home/esp/esp2/mission/2018_08_31_niagara_mission.rb:98] begun 11:30:04 Preparing to sleep until 7:12AM 9/13/2018 Asleep til 7:12AM 9/13/2018 Continuing at 07:12EDT13-Sep-18 @13:40:30.13 Shutdown @07:12:04.90EDT13-Sep-18 Phase [Sample.deep; initialPurge; da withoutWCR] @07:12:06.40 Starting initialPurge Loading FlushPuck into Collection Clamp from FlushPuck::Garage @07:12:22.60 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @07:13:08.24 Purging external Intake @07:13:32.26 Flushing upper manifold @07:14:49.06 Garaging FlushPuck in Collection Clamp @07:15:02.16 da sampling at most 1000ml Loading sh1 puck from tube 3 into Collection Clamp @07:15:21.57 collectSample 1000ml w/bubble point of 24psi Priming 7ml @07:15:21.64 Calibrating pressure and temperature sensors @07:15:35.02 Waiting for external sampler to finish priming... @07:18:35.66 Sampling 1000.0ml @07:43:55.18 Sampled 1000.0ml Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @07:44:42.74 BubblePoint: Intake @14.7psia, Outlet @14.7psia @07:45:03.06 Evacuated: Intake @14.7psia, Outlet @14.7psia @07:45:31.11 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @07:46:17.19 BubblePoint: Intake @14.7psia, Outlet @14.7psia @07:46:37.50 Evacuated: Intake @14.7psia, Outlet @14.7psia @07:47:05.56 Lysing: Adding 2.0ml AqMeOH @07:48:13.38 Heating sh1 puck from tube 3 in Collection Clamp to 70C for 10:00 @07:59:39.38 Collection Puck Heater at 70C after lysing @08:00:20.75 Recovering Lysate into Collection syringe (recycling once) @08:01:01.93 Recycling Lysate (#1 of 1) @08:02:10.15 Recovering Lysate (#2 of 2) @08:02:51.33 Lysing Complete Storing sh1 puck in Collection Clamp to tube 2 @08:02:58.71 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:03:12.82 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:03:15.16 Tranferring DA Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:06:52.24 Garaging FlushPuck in Collection Clamp @08:07:05.34 Loading lyfil puck from tube 3 into Collection Clamp @08:09:35.44 Storing lyfil puck in Collection Clamp to tube 2 @08:09:42.72 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:09:56.83 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:10:59.06 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @08:12:17.62 Garaging FlushPuck in Processing Clamp @08:12:29.47 Loading da puck from tube 3 into Processing Clamp @08:12:50.25 Heating da puck from tube 3 in Processing Clamp to 28C for 2:00:00 @08:20:29.78 recoverPSRreagent08 @08:22:16.98 addPMLReagent08 @08:22:59.05 Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:26:35.62 Garaging FlushPuck in Collection Clamp @08:30:59.05 recoverPRVreagent08 @08:31:59.31 washCycleDA #1 of 2 @08:34:54.96 recoverPSRreagent08 @08:36:05.35 washCycleDA #2 of 2 @08:39:00.24 recoverPSRreagent08 @08:40:10.63 getPMLReagent08 @08:40:32.87 addPMLReagent08 @08:49:14.44 recoverPRVreagent08 @08:50:13.95 washCycleDA #1 of 3 @08:53:09.59 recoverPSRreagent08 @08:54:19.98 washCycleDA #2 of 3 @08:57:14.87 recoverPSRreagent08 @08:58:25.26 washCycleDA #3 of 3 @09:01:20.16 recoverPSRreagent08 @09:02:30.55 Mixing DA Substrates @09:03:41.29 recoverPSRreagent08 @09:04:51.68 Processing Puck Heater at 28C before imaging Imaging da puck in Processing Clamp while storing it to tube 2 @09:04:59.09 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @09:05:15.94 Lights, camera, ACTION! Simulating PuckCamera: snap -bin=2,2 -auto /var/log/esp/da18sep1309h1000ml.tif @09:05:35.94 Waiting >20 seconds for da to relinquish FlushPuck @09:07:05.94 Simulating PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da18sep1309h1000ml15s.tif @09:07:30.94 Simulating PuckCamera: snap -bin=2,2 30.0 /var/log/esp/midres/da18sep1309h1000ml30s.tif @09:08:10.94 Simulating PuckCamera: snap -bin=1,1 70.0 /var/log/esp/hires/da18sep1309h1000ml70s.tif @09:09:32.10 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @09:09:34.44 Loading FlushPuck into Processing Clamp from FlushPuck::Garage Starting finalPurge @09:09:54.44 Waiting >20 seconds for psFlush to relinquish FlushPuck @09:18:52.78 Loading FlushPuck into Collection Clamp from Processing Clamp @09:19:10.62 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @09:19:55.14 Flushing lower manifold's waste1 port @09:20:19.19 Flushing lower manifold's waste2 port @09:20:43.24 Disinfecting external Intake with kill @09:22:16.73 Garaging FlushPuck in Collection Clamp @09:22:29.83 Completed [Sample.deep; initialPurge; da withoutWCR] begun 07:12:04 Preparing to sleep until 11:30AM 9/13/2018 Asleep til 11:30AM 9/13/2018 Continuing at 11:30 @09:22:30.13 Shutdown @11:30:04.90 Phase [/home/esp/esp2/mission/2018_08_31_niagara_mission.rb:107] @11:30:06.40 Starting initialPurge Loading FlushPuck into Collection Clamp from FlushPuck::Garage @11:30:22.60 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @11:31:08.24 Purging external Intake @11:31:32.26 Flushing upper manifold @11:32:49.06 Garaging FlushPuck in Collection Clamp @11:33:02.16 da sampling at most 1000ml Loading sh1 puck from tube 3 into Collection Clamp @11:33:21.85 collectSample 1000ml w/bubble point of 24psi Priming 7ml @11:33:21.92 Calibrating pressure and temperature sensors @11:33:35.31 Waiting for external sampler to finish priming... @11:36:35.94 Sampling 1000.0ml @12:01:55.46 Sampled 1000.0ml Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @12:02:43.03 BubblePoint: Intake @14.7psia, Outlet @14.7psia @12:03:03.34 Evacuated: Intake @14.7psia, Outlet @14.7psia @12:03:31.40 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @12:04:17.47 BubblePoint: Intake @14.7psia, Outlet @14.7psia @12:04:37.79 Evacuated: Intake @14.7psia, Outlet @14.7psia @12:05:05.84 Lysing: Adding 2.0ml AqMeOH @12:06:13.66 Heating sh1 puck from tube 3 in Collection Clamp to 70C for 10:00 @12:17:39.66 Collection Puck Heater at 70C after lysing @12:18:21.03 Recovering Lysate into Collection syringe (recycling once) @12:19:02.21 Recycling Lysate (#1 of 1) @12:20:10.43 Recovering Lysate (#2 of 2) @12:20:51.61 Lysing Complete Storing sh1 puck in Collection Clamp to tube 2 @12:20:58.69 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:21:12.81 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:21:15.14 Tranferring DA Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:24:52.23 Garaging FlushPuck in Collection Clamp @12:25:05.33 Loading lyfil puck from tube 3 into Collection Clamp @12:27:35.73 Storing lyfil puck in Collection Clamp to tube 2 @12:27:42.70 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:27:56.81 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:28:59.04 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @12:30:17.60 Garaging FlushPuck in Processing Clamp @12:30:29.45 Loading da puck from tube 4 into Processing Clamp @12:30:51.52 Heating da puck from tube 4 in Processing Clamp to 28C for 2:00:00 @12:38:31.04 recoverPSRreagent08 @12:40:18.25 addPMLReagent08 @12:41:00.32 Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:44:36.88 Garaging FlushPuck in Collection Clamp @12:49:00.32 recoverPRVreagent08 @12:50:00.58 washCycleDA #1 of 2 @12:52:56.22 recoverPSRreagent08 @12:54:06.61 washCycleDA #2 of 2 @12:57:01.51 recoverPSRreagent08 @12:58:11.90 getPMLReagent08 @12:58:34.13 addPMLReagent08 @13:07:15.70 recoverPRVreagent08 @13:08:15.21 washCycleDA #1 of 3 @13:11:10.86 recoverPSRreagent08 @13:12:21.25 washCycleDA #2 of 3 @13:15:16.14 recoverPSRreagent08 @13:16:26.53 washCycleDA #3 of 3 @13:19:21.42 recoverPSRreagent08 @13:20:31.81 Mixing DA Substrates @13:21:42.55 recoverPSRreagent08 @13:22:52.94 Processing Puck Heater at 28C before imaging Imaging da puck in Processing Clamp while storing it to tube 3 @13:23:09.59 WARNING: Will not reach camera seal! @13:23:09.72 Lights, camera, ACTION! Simulating PuckCamera: snap -bin=2,2 -auto /var/log/esp/da18sep1313h1000ml.tif @13:23:29.72 Waiting >20 seconds for da to relinquish FlushPuck @13:24:59.72 Simulating PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da18sep1313h1000ml15s.tif @13:25:24.72 Simulating PuckCamera: snap -bin=2,2 30.0 /var/log/esp/midres/da18sep1313h1000ml30s.tif @13:26:04.72 Simulating PuckCamera: snap -bin=1,1 70.0 /var/log/esp/hires/da18sep1313h1000ml70s.tif @13:27:25.88 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @13:27:28.94 Loading FlushPuck into Processing Clamp from FlushPuck::Garage Starting finalPurge @13:27:48.94 Waiting >20 seconds for psFlush to relinquish FlushPuck @13:36:47.28 Loading FlushPuck into Collection Clamp from Processing Clamp @13:37:05.12 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @13:37:49.64 Flushing lower manifold's waste1 port @13:38:13.69 Flushing lower manifold's waste2 port @13:38:37.74 Disinfecting external Intake with kill @13:40:11.23 Garaging FlushPuck in Collection Clamp @13:40:24.33 Completed [/home/esp/esp2/mission/2018_08_31_niagara_mission.rb:107] begun 11:30:04 Preparing to sleep until 7:14AM 9/15/2018 Asleep til 7:14AM 9/15/2018 Continuing at 07:14EDT15-Sep-18 @13:40:24.63 Shutdown @07:14:08.25EDT15-Sep-18 Phase [Sample.deep; initialPurge; da withoutWCR] @07:14:09.75 Starting initialPurge Loading FlushPuck into Collection Clamp from FlushPuck::Garage @07:14:25.94 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @07:15:11.59 Purging external Intake @07:15:35.61 Flushing upper manifold @07:16:52.40 Garaging FlushPuck in Collection Clamp @07:17:05.50 da sampling at most 1000ml Loading sh1 puck from tube 4 into Collection Clamp @07:17:26.48 collectSample 1000ml w/bubble point of 24psi Priming 7ml @07:17:26.54 Calibrating pressure and temperature sensors @07:17:39.93 Waiting for external sampler to finish priming... @07:20:40.57 Sampling 1000.0ml @07:46:00.09 Sampled 1000.0ml Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @07:46:47.65 BubblePoint: Intake @14.7psia, Outlet @14.7psia @07:47:07.97 Evacuated: Intake @14.7psia, Outlet @14.7psia @07:47:36.02 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @07:48:22.09 BubblePoint: Intake @14.7psia, Outlet @14.7psia @07:48:42.41 Evacuated: Intake @14.7psia, Outlet @14.7psia @07:49:10.46 Lysing: Adding 2.0ml AqMeOH @07:50:18.29 Heating sh1 puck from tube 4 in Collection Clamp to 70C for 10:00 @08:01:44.29 Collection Puck Heater at 70C after lysing @08:02:25.65 Recovering Lysate into Collection syringe (recycling once) @08:03:06.83 Recycling Lysate (#1 of 1) @08:04:15.06 Recovering Lysate (#2 of 2) @08:04:56.24 Lysing Complete Storing sh1 puck in Collection Clamp to tube 3 @08:05:05.21 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:05:19.32 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:05:22.27 Tranferring DA Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:08:59.35 Garaging FlushPuck in Collection Clamp @08:09:12.45 Loading lyfil puck from tube 4 into Collection Clamp @08:11:40.99 Storing lyfil puck in Collection Clamp to tube 3 @08:11:49.86 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:12:03.97 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:13:06.71 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @08:14:25.27 Garaging FlushPuck in Processing Clamp @08:14:37.12 Loading da puck from tube 4 into Processing Clamp @08:14:56.35 Heating da puck from tube 4 in Processing Clamp to 28C for 2:00:00 @08:22:35.87 recoverPSRreagent08 @08:24:23.07 addPMLReagent08 @08:25:05.15 Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:28:41.71 Garaging FlushPuck in Collection Clamp @08:33:05.15 recoverPRVreagent08 @08:34:05.41 washCycleDA #1 of 2 @08:37:01.05 recoverPSRreagent08 @08:38:11.44 washCycleDA #2 of 2 @08:41:06.33 recoverPSRreagent08 @08:42:16.72 getPMLReagent08 @08:42:38.96 addPMLReagent08 @08:51:20.53 recoverPRVreagent08 @08:52:20.04 washCycleDA #1 of 3 @08:55:15.69 recoverPSRreagent08 @08:56:26.08 washCycleDA #2 of 3 @08:59:20.97 recoverPSRreagent08 @09:00:31.36 washCycleDA #3 of 3 @09:03:26.25 recoverPSRreagent08 @09:04:36.64 Mixing DA Substrates @09:05:47.38 recoverPSRreagent08 @09:06:57.77 Processing Puck Heater at 28C before imaging Imaging da puck in Processing Clamp while storing it to tube 3 @09:07:06.78 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @09:07:23.63 Lights, camera, ACTION! Simulating PuckCamera: snap -bin=2,2 -auto /var/log/esp/da18sep1509h1000ml.tif @09:07:43.63 Waiting >20 seconds for da to relinquish FlushPuck @09:09:13.63 Simulating PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da18sep1509h1000ml15s.tif @09:09:38.63 Simulating PuckCamera: snap -bin=2,2 30.0 /var/log/esp/midres/da18sep1509h1000ml30s.tif @09:10:18.63 Simulating PuckCamera: snap -bin=1,1 70.0 /var/log/esp/hires/da18sep1509h1000ml70s.tif @09:11:39.79 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @09:11:42.55 Loading FlushPuck into Processing Clamp from FlushPuck::Garage Starting finalPurge @09:12:02.55 Waiting >20 seconds for psFlush to relinquish FlushPuck @09:21:00.89 Loading FlushPuck into Collection Clamp from Processing Clamp @09:21:18.73 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @09:22:03.25 Flushing lower manifold's waste1 port @09:22:27.30 Flushing lower manifold's waste2 port @09:22:51.35 Disinfecting external Intake with kill @09:24:24.84 Garaging FlushPuck in Collection Clamp @09:24:37.94 Completed [Sample.deep; initialPurge; da withoutWCR] begun 07:14:08 Preparing to sleep until 11:30AM 9/15/2018 Asleep til 11:30AM 9/15/2018 Continuing at 11:30 @09:24:38.24 Shutdown @11:30:08.25 Phase [/home/esp/esp2/mission/2018_08_31_niagara_mission.rb:117] @11:30:09.75 Starting initialPurge Loading FlushPuck into Collection Clamp from FlushPuck::Garage @11:30:25.94 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @11:31:11.59 Purging external Intake @11:31:35.61 Flushing upper manifold @11:32:52.40 Garaging FlushPuck in Collection Clamp @11:33:05.50 da sampling at most 1000ml Loading sh1 puck from tube 4 into Collection Clamp @11:33:26.77 collectSample 1000ml w/bubble point of 24psi Priming 7ml @11:33:26.84 Calibrating pressure and temperature sensors @11:33:40.23 Waiting for external sampler to finish priming... @11:36:40.86 Sampling 1000.0ml @12:02:00.38 Sampled 1000.0ml Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @12:02:47.95 BubblePoint: Intake @14.7psia, Outlet @14.7psia @12:03:08.26 Evacuated: Intake @14.7psia, Outlet @14.7psia @12:03:36.32 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @12:04:22.39 BubblePoint: Intake @14.7psia, Outlet @14.7psia @12:04:42.71 Evacuated: Intake @14.7psia, Outlet @14.7psia @12:05:10.76 Lysing: Adding 2.0ml AqMeOH @12:06:18.59 Heating sh1 puck from tube 4 in Collection Clamp to 70C for 10:00 @12:17:44.59 Collection Puck Heater at 70C after lysing @12:18:25.95 Recovering Lysate into Collection syringe (recycling once) @12:19:07.13 Recycling Lysate (#1 of 1) @12:20:15.35 Recovering Lysate (#2 of 2) @12:20:56.53 Lysing Complete Storing sh1 puck in Collection Clamp to tube 3 @12:21:05.21 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:21:19.32 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:21:21.97 Tranferring DA Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:24:59.05 Garaging FlushPuck in Collection Clamp @12:25:12.15 Loading lyfil puck from tube 4 into Collection Clamp @12:27:40.99 Storing lyfil puck in Collection Clamp to tube 3 @12:27:49.58 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:28:03.69 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:29:06.13 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @12:30:24.70 Garaging FlushPuck in Processing Clamp @12:30:36.55 Loading da puck from tube 4 into Processing Clamp @12:30:56.06 Heating da puck from tube 4 in Processing Clamp to 28C for 2:00:00 @12:38:35.59 recoverPSRreagent08 @12:40:22.79 addPMLReagent08 @12:41:04.86 Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:44:41.43 Garaging FlushPuck in Collection Clamp @12:49:04.86 recoverPRVreagent08 @12:50:05.13 washCycleDA #1 of 2 @12:53:00.77 recoverPSRreagent08 @12:54:11.16 washCycleDA #2 of 2 @12:57:06.05 recoverPSRreagent08 @12:58:16.44 getPMLReagent08 @12:58:38.68 addPMLReagent08 @13:07:20.25 recoverPRVreagent08 @13:08:19.76 washCycleDA #1 of 3 @13:11:15.40 recoverPSRreagent08 @13:12:25.79 washCycleDA #2 of 3 @13:15:20.69 recoverPSRreagent08 @13:16:31.08 washCycleDA #3 of 3 @13:19:25.97 recoverPSRreagent08 @13:20:36.36 Mixing DA Substrates @13:21:47.10 recoverPSRreagent08 @13:22:57.49 Processing Puck Heater at 28C before imaging Imaging da puck in Processing Clamp while storing it to tube 3 @13:23:06.20 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @13:23:23.05 Lights, camera, ACTION! Simulating PuckCamera: snap -bin=2,2 -auto /var/log/esp/da18sep1513h1000ml.tif @13:23:43.05 Waiting >20 seconds for da to relinquish FlushPuck @13:25:13.05 Simulating PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da18sep1513h1000ml15s.tif @13:25:38.05 Simulating PuckCamera: snap -bin=2,2 30.0 /var/log/esp/midres/da18sep1513h1000ml30s.tif @13:26:18.05 Simulating PuckCamera: snap -bin=1,1 70.0 /var/log/esp/hires/da18sep1513h1000ml70s.tif @13:27:39.21 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @13:27:41.67 Starting finalPurge Loading FlushPuck into Processing Clamp from FlushPuck::Garage @13:28:01.67 Waiting >20 seconds for psFlush to relinquish FlushPuck @13:37:00.02 Loading FlushPuck into Collection Clamp from Processing Clamp @13:37:17.86 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @13:38:02.38 Flushing lower manifold's waste1 port @13:38:26.43 Flushing lower manifold's waste2 port @13:38:50.48 Disinfecting external Intake with kill @13:40:23.97 Garaging FlushPuck in Collection Clamp @13:40:37.07 Completed [/home/esp/esp2/mission/2018_08_31_niagara_mission.rb:117] begun 11:30:08 Preparing to sleep until 7:16AM 9/17/2018 Asleep til 7:16AM 9/17/2018 Continuing at 07:16EDT17-Sep-18 @13:40:37.37 Shutdown @07:16:08.25EDT17-Sep-18 Phase [Sample.deep; initialPurge; da withoutWCR] @07:16:09.75 Starting initialPurge Loading FlushPuck into Collection Clamp from FlushPuck::Garage @07:16:25.94 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @07:17:11.59 Purging external Intake @07:17:35.61 Flushing upper manifold @07:18:52.40 Garaging FlushPuck in Collection Clamp @07:19:05.50 da sampling at most 1000ml Loading sh1 puck from tube 4 into Collection Clamp @07:19:27.07 collectSample 1000ml w/bubble point of 24psi Priming 7ml @07:19:27.14 Calibrating pressure and temperature sensors @07:19:40.52 Waiting for external sampler to finish priming... @07:22:41.16 Sampling 1000.0ml @07:48:00.68 Sampled 1000.0ml Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @07:48:48.24 BubblePoint: Intake @14.7psia, Outlet @14.7psia @07:49:08.56 Evacuated: Intake @14.7psia, Outlet @14.7psia @07:49:36.61 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @07:50:22.69 BubblePoint: Intake @14.7psia, Outlet @14.7psia @07:50:43.00 Evacuated: Intake @14.7psia, Outlet @14.7psia @07:51:11.06 Lysing: Adding 2.0ml AqMeOH @07:52:18.88 Heating sh1 puck from tube 4 in Collection Clamp to 70C for 10:00 @08:03:44.88 Collection Puck Heater at 70C after lysing @08:04:26.25 Recovering Lysate into Collection syringe (recycling once) @08:05:07.43 Recycling Lysate (#1 of 1) @08:06:15.65 Recovering Lysate (#2 of 2) @08:06:56.83 Lysing Complete Storing sh1 puck in Collection Clamp to tube 3 @08:07:05.21 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:07:19.32 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:07:21.68 Tranferring DA Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:10:58.76 Garaging FlushPuck in Collection Clamp @08:11:11.86 Loading lyfil puck from tube 4 into Collection Clamp @08:13:40.99 Storing lyfil puck in Collection Clamp to tube 3 @08:13:49.28 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:14:03.39 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:15:05.62 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @08:16:24.18 Garaging FlushPuck in Processing Clamp @08:16:36.03 Loading da puck from tube 4 into Processing Clamp @08:16:55.85 Heating da puck from tube 4 in Processing Clamp to 28C for 2:00:00 @08:24:35.37 recoverPSRreagent08 @08:26:22.57 addPMLReagent08 @08:27:04.65 Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:30:41.21 Garaging FlushPuck in Collection Clamp @08:35:04.65 recoverPRVreagent08 @08:36:04.91 washCycleDA #1 of 2 @08:39:00.55 recoverPSRreagent08 @08:40:10.94 washCycleDA #2 of 2 @08:43:05.83 recoverPSRreagent08 @08:44:16.22 getPMLReagent08 @08:44:38.46 addPMLReagent08 @08:53:20.03 recoverPRVreagent08 @08:54:19.54 washCycleDA #1 of 3 @08:57:15.19 recoverPSRreagent08 @08:58:25.58 washCycleDA #2 of 3 @09:01:20.47 recoverPSRreagent08 @09:02:30.86 washCycleDA #3 of 3 @09:05:25.75 recoverPSRreagent08 @09:06:36.14 Mixing DA Substrates @09:07:46.88 recoverPSRreagent08 @09:08:57.27 Processing Puck Heater at 28C before imaging Imaging da puck in Processing Clamp while storing it to tube 3 @09:09:05.68 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @09:09:22.53 Lights, camera, ACTION! Simulating PuckCamera: snap -bin=2,2 -auto /var/log/esp/da18sep1709h1000ml.tif @09:09:42.53 Waiting >20 seconds for da to relinquish FlushPuck @09:11:12.53 Simulating PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da18sep1709h1000ml15s.tif @09:11:37.53 Simulating PuckCamera: snap -bin=2,2 30.0 /var/log/esp/midres/da18sep1709h1000ml30s.tif @09:12:17.53 Simulating PuckCamera: snap -bin=1,1 70.0 /var/log/esp/hires/da18sep1709h1000ml70s.tif @09:13:38.69 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @09:13:41.03 Loading FlushPuck into Processing Clamp from FlushPuck::Garage Starting finalPurge @09:14:01.03 Waiting >20 seconds for psFlush to relinquish FlushPuck @09:22:59.38 Loading FlushPuck into Collection Clamp from Processing Clamp @09:23:17.22 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @09:24:01.74 Flushing lower manifold's waste1 port @09:24:25.79 Flushing lower manifold's waste2 port @09:24:49.84 Disinfecting external Intake with kill @09:26:23.33 Garaging FlushPuck in Collection Clamp @09:26:36.43 Completed [Sample.deep; initialPurge; da withoutWCR] begun 07:16:08 Preparing to sleep until 11:30AM 9/17/2018 Asleep til 11:30AM 9/17/2018 Continuing at 11:30 @09:26:36.73 Shutdown @11:30:08.25 Phase [/home/esp/esp2/mission/2018_08_31_niagara_mission.rb:126] @11:30:09.75 Starting initialPurge Loading FlushPuck into Collection Clamp from FlushPuck::Garage @11:30:25.94 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @11:31:11.59 Purging external Intake @11:31:35.61 Flushing upper manifold @11:32:52.40 Garaging FlushPuck in Collection Clamp @11:33:05.50 da sampling at most 1000ml Loading sh1 puck from tube 4 into Collection Clamp @11:33:27.37 collectSample 1000ml w/bubble point of 24psi Priming 7ml @11:33:27.43 Calibrating pressure and temperature sensors @11:33:40.82 Waiting for external sampler to finish priming... @11:36:41.46 Sampling 1000.0ml @12:02:00.98 Sampled 1000.0ml Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @12:02:48.54 BubblePoint: Intake @14.7psia, Outlet @14.7psia @12:03:08.86 Evacuated: Intake @14.7psia, Outlet @14.7psia @12:03:36.91 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @12:04:22.98 BubblePoint: Intake @14.7psia, Outlet @14.7psia @12:04:43.30 Evacuated: Intake @14.7psia, Outlet @14.7psia @12:05:11.35 Lysing: Adding 2.0ml AqMeOH @12:06:19.18 Heating sh1 puck from tube 4 in Collection Clamp to 70C for 10:00 @12:17:45.18 Collection Puck Heater at 70C after lysing @12:18:26.54 Recovering Lysate into Collection syringe (recycling once) @12:19:07.72 Recycling Lysate (#1 of 1) @12:20:15.95 Recovering Lysate (#2 of 2) @12:20:57.13 Lysing Complete Storing sh1 puck in Collection Clamp to tube 3 @12:21:05.21 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:21:19.32 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:21:21.66 Tranferring DA Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:24:58.74 Garaging FlushPuck in Collection Clamp @12:25:11.84 Loading lyfil puck from tube 4 into Collection Clamp @12:27:41.27 Storing lyfil puck in Collection Clamp to tube 3 @12:27:49.26 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:28:03.38 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:29:05.60 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @12:30:24.16 Garaging FlushPuck in Processing Clamp @12:30:36.01 Loading da puck from tube 4 into Processing Clamp @12:30:56.13 Heating da puck from tube 4 in Processing Clamp to 28C for 2:00:00 @12:38:35.65 recoverPSRreagent08 @12:40:22.85 addPMLReagent08 @12:41:04.93 Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:44:41.49 Garaging FlushPuck in Collection Clamp @12:49:04.93 recoverPRVreagent08 @12:50:05.19 washCycleDA #1 of 2 @12:53:00.83 recoverPSRreagent08 @12:54:11.22 washCycleDA #2 of 2 @12:57:06.12 recoverPSRreagent08 @12:58:16.51 getPMLReagent08 @12:58:38.74 addPMLReagent08 @13:07:20.31 recoverPRVreagent08 @13:08:19.82 washCycleDA #1 of 3 @13:11:15.47 recoverPSRreagent08 @13:12:25.86 washCycleDA #2 of 3 @13:15:20.75 recoverPSRreagent08 @13:16:31.14 washCycleDA #3 of 3 @13:19:26.03 recoverPSRreagent08 @13:20:36.42 Mixing DA Substrates @13:21:47.16 recoverPSRreagent08 @13:22:57.55 Processing Puck Heater at 28C before imaging Imaging da puck in Processing Clamp while storing it to tube 3 @13:23:05.67 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @13:23:22.52 Lights, camera, ACTION! Simulating PuckCamera: snap -bin=2,2 -auto /var/log/esp/da18sep1713h1000ml.tif @13:23:42.52 Waiting >20 seconds for da to relinquish FlushPuck @13:25:12.52 Simulating PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da18sep1713h1000ml15s.tif @13:25:37.52 Simulating PuckCamera: snap -bin=2,2 30.0 /var/log/esp/midres/da18sep1713h1000ml30s.tif @13:26:17.52 Simulating PuckCamera: snap -bin=1,1 70.0 /var/log/esp/hires/da18sep1713h1000ml70s.tif @13:27:38.68 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @13:27:41.02 Starting finalPurge Loading FlushPuck into Processing Clamp from FlushPuck::Garage @13:28:01.02 Waiting >20 seconds for psFlush to relinquish FlushPuck @13:36:59.36 Loading FlushPuck into Collection Clamp from Processing Clamp @13:37:17.20 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @13:38:01.72 Flushing lower manifold's waste1 port @13:38:25.77 Flushing lower manifold's waste2 port @13:38:49.82 Disinfecting external Intake with kill @13:40:23.31 Garaging FlushPuck in Collection Clamp @13:40:36.41 Completed [/home/esp/esp2/mission/2018_08_31_niagara_mission.rb:126] begun 11:30:08 Preparing to sleep until 7:18AM 9/19/2018 Asleep til 7:18AM 9/19/2018 Continuing at 07:18EDT19-Sep-18 @13:40:36.71 Shutdown @07:18:08.25EDT19-Sep-18 Phase [Sample.deep; initialPurge; da withoutWCR] @07:18:09.75 Starting initialPurge Loading FlushPuck into Collection Clamp from FlushPuck::Garage @07:18:25.94 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @07:19:11.59 Purging external Intake @07:19:35.61 Flushing upper manifold @07:20:52.40 Garaging FlushPuck in Collection Clamp @07:21:05.50 da sampling at most 1000ml Loading sh1 puck from tube 4 into Collection Clamp @07:21:27.66 collectSample 1000ml w/bubble point of 24psi Priming 7ml @07:21:27.73 Calibrating pressure and temperature sensors @07:21:41.12 Waiting for external sampler to finish priming... @07:24:41.75 Sampling 1000.0ml @07:50:01.27 Sampled 1000.0ml Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @07:50:48.84 BubblePoint: Intake @14.7psia, Outlet @14.7psia @07:51:09.15 Evacuated: Intake @14.7psia, Outlet @14.7psia @07:51:37.21 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @07:52:23.28 BubblePoint: Intake @14.7psia, Outlet @14.7psia @07:52:43.60 Evacuated: Intake @14.7psia, Outlet @14.7psia @07:53:11.65 Lysing: Adding 2.0ml AqMeOH @07:54:19.48 Heating sh1 puck from tube 4 in Collection Clamp to 70C for 10:00 @08:05:45.48 Collection Puck Heater at 70C after lysing @08:06:26.84 Recovering Lysate into Collection syringe (recycling once) @08:07:08.02 Recycling Lysate (#1 of 1) @08:08:16.24 Recovering Lysate (#2 of 2) @08:08:57.42 Lysing Complete Storing sh1 puck in Collection Clamp to tube 3 @08:09:05.21 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:09:19.32 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:09:21.66 Tranferring DA Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:12:58.74 Garaging FlushPuck in Collection Clamp @08:13:11.84 Loading lyfil puck from tube 4 into Collection Clamp @08:15:41.57 Storing lyfil puck in Collection Clamp to tube 3 @08:15:49.26 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:16:03.38 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:17:05.60 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @08:18:24.16 Garaging FlushPuck in Processing Clamp @08:18:36.01 Loading da puck from tube 4 into Processing Clamp @08:18:56.42 Heating da puck from tube 4 in Processing Clamp to 28C for 2:00:00 @08:26:35.95 recoverPSRreagent08 @08:28:23.15 addPMLReagent08 @08:29:05.22 Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:32:41.79 Garaging FlushPuck in Collection Clamp @08:37:05.22 recoverPRVreagent08 @08:38:05.48 washCycleDA #1 of 2 @08:41:01.13 recoverPSRreagent08 @08:42:11.52 washCycleDA #2 of 2 @08:45:06.41 recoverPSRreagent08 @08:46:16.80 getPMLReagent08 @08:46:39.04 addPMLReagent08 @08:55:20.61 recoverPRVreagent08 @08:56:20.12 washCycleDA #1 of 3 @08:59:15.76 recoverPSRreagent08 @09:00:26.15 washCycleDA #2 of 3 @09:03:21.05 recoverPSRreagent08 @09:04:31.44 washCycleDA #3 of 3 @09:07:26.33 recoverPSRreagent08 @09:08:36.72 Mixing DA Substrates @09:09:47.46 recoverPSRreagent08 @09:10:57.85 Processing Puck Heater at 28C before imaging Imaging da puck in Processing Clamp while storing it to tube 3 @09:11:05.67 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @09:11:22.52 Lights, camera, ACTION! Simulating PuckCamera: snap -bin=2,2 -auto /var/log/esp/da18sep1909h1000ml.tif @09:11:42.52 Waiting >20 seconds for da to relinquish FlushPuck @09:13:12.52 Simulating PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da18sep1909h1000ml15s.tif @09:13:37.52 Simulating PuckCamera: snap -bin=2,2 30.0 /var/log/esp/midres/da18sep1909h1000ml30s.tif @09:14:17.52 Simulating PuckCamera: snap -bin=1,1 70.0 /var/log/esp/hires/da18sep1909h1000ml70s.tif @09:15:38.68 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @09:15:41.02 Loading FlushPuck into Processing Clamp from FlushPuck::Garage Starting finalPurge @09:16:01.02 Waiting >20 seconds for psFlush to relinquish FlushPuck @09:24:59.36 Loading FlushPuck into Collection Clamp from Processing Clamp @09:25:17.20 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @09:26:01.72 Flushing lower manifold's waste1 port @09:26:25.77 Flushing lower manifold's waste2 port @09:26:49.82 Disinfecting external Intake with kill @09:28:23.31 Garaging FlushPuck in Collection Clamp @09:28:36.41 Completed [Sample.deep; initialPurge; da withoutWCR] begun 07:18:08 Preparing to sleep until 11:30AM 9/19/2018 Asleep til 11:30AM 9/19/2018 Continuing at 11:30 @09:28:36.71 Shutdown @11:30:08.25 Phase [/home/esp/esp2/mission/2018_08_31_niagara_mission.rb:135] @11:30:09.75 Starting initialPurge Loading FlushPuck into Collection Clamp from FlushPuck::Garage @11:30:25.94 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @11:31:11.59 Purging external Intake @11:31:35.61 Flushing upper manifold @11:32:52.40 Garaging FlushPuck in Collection Clamp @11:33:05.50 da sampling at most 1000ml Loading sh1 puck from tube 4 into Collection Clamp @11:33:27.96 collectSample 1000ml w/bubble point of 24psi Priming 7ml @11:33:28.03 Calibrating pressure and temperature sensors @11:33:41.42 Waiting for external sampler to finish priming... @11:36:42.05 Sampling 1000.0ml @12:02:01.57 Sampled 1000.0ml Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @12:02:49.13 BubblePoint: Intake @14.7psia, Outlet @14.7psia @12:03:09.45 Evacuated: Intake @14.7psia, Outlet @14.7psia @12:03:37.51 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @12:04:23.58 BubblePoint: Intake @14.7psia, Outlet @14.7psia @12:04:43.89 Evacuated: Intake @14.7psia, Outlet @14.7psia @12:05:11.95 Lysing: Adding 2.0ml AqMeOH @12:06:19.77 Heating sh1 puck from tube 4 in Collection Clamp to 70C for 10:00 @12:17:45.77 Collection Puck Heater at 70C after lysing @12:18:27.14 Recovering Lysate into Collection syringe (recycling once) @12:19:08.32 Recycling Lysate (#1 of 1) @12:20:16.54 Recovering Lysate (#2 of 2) @12:20:57.72 Lysing Complete Storing sh1 puck in Collection Clamp to tube 3 @12:21:05.21 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:21:19.32 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:21:21.66 Tranferring DA Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:24:58.74 Garaging FlushPuck in Collection Clamp @12:25:11.84 Loading lyfil puck from tube 4 into Collection Clamp @12:27:41.87 Storing lyfil puck in Collection Clamp to tube 3 @12:27:49.26 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:28:03.38 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:29:05.60 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @12:30:24.16 Garaging FlushPuck in Processing Clamp @12:30:36.01 Loading da puck from tube 4 into Processing Clamp @12:30:56.72 Heating da puck from tube 4 in Processing Clamp to 28C for 2:00:00 @12:38:36.25 recoverPSRreagent08 @12:40:23.45 addPMLReagent08 @12:41:05.52 Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:44:42.09 Garaging FlushPuck in Collection Clamp @12:49:05.52 recoverPRVreagent08 @12:50:05.78 washCycleDA #1 of 2 @12:53:01.43 recoverPSRreagent08 @12:54:11.82 washCycleDA #2 of 2 @12:57:06.71 recoverPSRreagent08 @12:58:17.10 getPMLReagent08 @12:58:39.34 addPMLReagent08 @13:07:20.91 recoverPRVreagent08 @13:08:20.42 washCycleDA #1 of 3 @13:11:16.06 recoverPSRreagent08 @13:12:26.45 washCycleDA #2 of 3 @13:15:21.34 recoverPSRreagent08 @13:16:31.73 washCycleDA #3 of 3 @13:19:26.63 recoverPSRreagent08 @13:20:37.02 Mixing DA Substrates @13:21:47.76 recoverPSRreagent08 @13:22:58.15 Processing Puck Heater at 28C before imaging Imaging da puck in Processing Clamp while storing it to tube 3 @13:23:05.67 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @13:23:22.52 Lights, camera, ACTION! Simulating PuckCamera: snap -bin=2,2 -auto /var/log/esp/da18sep1913h1000ml.tif @13:23:42.52 Waiting >20 seconds for da to relinquish FlushPuck @13:25:12.52 Simulating PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da18sep1913h1000ml15s.tif @13:25:37.52 Simulating PuckCamera: snap -bin=2,2 30.0 /var/log/esp/midres/da18sep1913h1000ml30s.tif @13:26:17.52 Simulating PuckCamera: snap -bin=1,1 70.0 /var/log/esp/hires/da18sep1913h1000ml70s.tif @13:27:38.68 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @13:27:41.02 Loading FlushPuck into Processing Clamp from FlushPuck::Garage Starting finalPurge @13:28:01.02 Waiting >20 seconds for psFlush to relinquish FlushPuck @13:36:59.36 Loading FlushPuck into Collection Clamp from Processing Clamp @13:37:17.20 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @13:38:01.72 Flushing lower manifold's waste1 port @13:38:25.77 Flushing lower manifold's waste2 port @13:38:49.82 Disinfecting external Intake with kill @13:40:23.31 Garaging FlushPuck in Collection Clamp @13:40:36.41 Completed [/home/esp/esp2/mission/2018_08_31_niagara_mission.rb:135] begun 11:30:08 Preparing to sleep until 7:20AM 9/21/2018 Asleep til 7:20AM 9/21/2018 Continuing at 07:20EDT21-Sep-18 @13:40:36.71 Shutdown @07:20:08.25EDT21-Sep-18 Phase [Sample.deep; initialPurge; da withoutWCR] @07:20:09.75 Starting initialPurge Loading FlushPuck into Collection Clamp from FlushPuck::Garage @07:20:25.94 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @07:21:11.59 Purging external Intake @07:21:35.61 Flushing upper manifold @07:22:52.40 Garaging FlushPuck in Collection Clamp @07:23:05.50 da sampling at most 1000ml Loading sh1 puck from tube 4 into Collection Clamp @07:23:28.26 collectSample 1000ml w/bubble point of 24psi Priming 7ml @07:23:28.32 Calibrating pressure and temperature sensors @07:23:41.71 Waiting for external sampler to finish priming... @07:26:42.35 Sampling 1000.0ml @07:52:01.87 Sampled 1000.0ml Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @07:52:49.43 BubblePoint: Intake @14.7psia, Outlet @14.7psia @07:53:09.75 Evacuated: Intake @14.7psia, Outlet @14.7psia @07:53:37.80 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @07:54:23.88 BubblePoint: Intake @14.7psia, Outlet @14.7psia @07:54:44.19 Evacuated: Intake @14.7psia, Outlet @14.7psia @07:55:12.25 Lysing: Adding 2.0ml AqMeOH @07:56:20.07 Heating sh1 puck from tube 4 in Collection Clamp to 70C for 10:00 @08:07:46.07 Collection Puck Heater at 70C after lysing @08:08:27.43 Recovering Lysate into Collection syringe (recycling once) @08:09:08.61 Recycling Lysate (#1 of 1) @08:10:16.84 Recovering Lysate (#2 of 2) @08:10:58.02 Lysing Complete Storing sh1 puck in Collection Clamp to tube 3 @08:11:05.21 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:11:19.32 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:11:21.66 Tranferring DA Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:14:58.74 Garaging FlushPuck in Collection Clamp @08:15:11.84 Loading lyfil puck from tube 4 into Collection Clamp @08:17:42.15 Storing lyfil puck in Collection Clamp to tube 3 @08:17:49.25 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:18:03.36 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:19:05.59 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @08:20:24.15 Garaging FlushPuck in Processing Clamp @08:20:36.00 Loading da puck from tube 4 into Processing Clamp @08:20:57.00 Heating da puck from tube 4 in Processing Clamp to 28C for 2:00:00 @08:28:36.53 recoverPSRreagent08 @08:30:23.73 addPMLReagent08 @08:31:05.80 Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:34:42.37 Garaging FlushPuck in Collection Clamp @08:39:05.80 recoverPRVreagent08 @08:40:06.06 washCycleDA #1 of 2 @08:43:01.71 recoverPSRreagent08 @08:44:12.10 washCycleDA #2 of 2 @08:47:06.99 recoverPSRreagent08 @08:48:17.38 getPMLReagent08 @08:48:39.62 addPMLReagent08 @08:57:21.19 recoverPRVreagent08 @08:58:20.70 washCycleDA #1 of 3 @09:01:16.34 recoverPSRreagent08 @09:02:26.73 washCycleDA #2 of 3 @09:05:21.62 recoverPSRreagent08 @09:06:32.01 washCycleDA #3 of 3 @09:09:26.91 recoverPSRreagent08 @09:10:37.30 Mixing DA Substrates @09:11:48.04 recoverPSRreagent08 @09:12:58.43 Processing Puck Heater at 28C before imaging Imaging da puck in Processing Clamp while storing it to tube 3 @09:13:05.65 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @09:13:22.50 Lights, camera, ACTION! Simulating PuckCamera: snap -bin=2,2 -auto /var/log/esp/da18sep2109h1000ml.tif @09:13:42.50 Waiting >20 seconds for da to relinquish FlushPuck @09:15:12.50 Simulating PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da18sep2109h1000ml15s.tif @09:15:37.50 Simulating PuckCamera: snap -bin=2,2 30.0 /var/log/esp/midres/da18sep2109h1000ml30s.tif @09:16:17.50 Simulating PuckCamera: snap -bin=1,1 70.0 /var/log/esp/hires/da18sep2109h1000ml70s.tif @09:17:38.66 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @09:17:41.00 Loading FlushPuck into Processing Clamp from FlushPuck::Garage Starting finalPurge @09:18:01.00 Waiting >20 seconds for psFlush to relinquish FlushPuck @09:26:59.34 Loading FlushPuck into Collection Clamp from Processing Clamp @09:27:17.19 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @09:28:01.71 Flushing lower manifold's waste1 port @09:28:25.76 Flushing lower manifold's waste2 port @09:28:49.81 Disinfecting external Intake with kill @09:30:23.29 Garaging FlushPuck in Collection Clamp @09:30:36.39 Completed [Sample.deep; initialPurge; da withoutWCR] begun 07:20:08 Preparing to sleep until 11:30AM 9/21/2018 Asleep til 11:30AM 9/21/2018 Continuing at 11:30 @09:30:36.69 Shutdown @11:30:08.25 Phase [/home/esp/esp2/mission/2018_08_31_niagara_mission.rb:144] @11:30:09.75 Starting initialPurge Loading FlushPuck into Collection Clamp from FlushPuck::Garage @11:30:25.94 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @11:31:11.59 Purging external Intake @11:31:35.61 Flushing upper manifold @11:32:52.40 Garaging FlushPuck in Collection Clamp @11:33:05.50 da sampling at most 1000ml Loading sh1 puck from tube 5 into Collection Clamp @11:33:29.52 collectSample 1000ml w/bubble point of 24psi Priming 7ml @11:33:29.59 Calibrating pressure and temperature sensors @11:33:42.98 Waiting for external sampler to finish priming... @11:36:43.61 Sampling 1000.0ml @12:02:03.13 Sampled 1000.0ml Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @12:02:50.70 BubblePoint: Intake @14.7psia, Outlet @14.7psia @12:03:11.01 Evacuated: Intake @14.7psia, Outlet @14.7psia @12:03:39.07 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @12:04:25.14 BubblePoint: Intake @14.7psia, Outlet @14.7psia @12:04:45.46 Evacuated: Intake @14.7psia, Outlet @14.7psia @12:05:13.51 Lysing: Adding 2.0ml AqMeOH @12:06:21.34 Heating sh1 puck from tube 5 in Collection Clamp to 70C for 10:00 @12:17:47.34 Collection Puck Heater at 70C after lysing @12:18:28.70 Recovering Lysate into Collection syringe (recycling once) @12:19:09.88 Recycling Lysate (#1 of 1) @12:20:18.10 Recovering Lysate (#2 of 2) @12:20:59.28 Lysing Complete Storing sh1 puck in Collection Clamp to tube 4 @12:21:13.76 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:21:16.91 Tranferring DA Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:24:53.99 Garaging FlushPuck in Collection Clamp @12:25:07.09 Loading lyfil puck from tube 5 into Collection Clamp @12:27:35.52 Storing lyfil puck in Collection Clamp to tube 4 @12:27:44.50 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:27:58.61 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:29:01.45 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @12:30:20.01 Garaging FlushPuck in Processing Clamp @12:30:31.86 Loading da puck from tube 5 into Processing Clamp @12:30:50.99 Heating da puck from tube 5 in Processing Clamp to 28C for 2:00:00 @12:38:30.51 recoverPSRreagent08 @12:40:17.71 addPMLReagent08 @12:40:59.79 Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:44:36.35 Garaging FlushPuck in Collection Clamp @12:48:59.79 recoverPRVreagent08 @12:50:00.05 washCycleDA #1 of 2 @12:52:55.69 recoverPSRreagent08 @12:54:06.08 washCycleDA #2 of 2 @12:57:00.97 recoverPSRreagent08 @12:58:11.36 getPMLReagent08 @12:58:33.60 addPMLReagent08 @13:07:15.17 recoverPRVreagent08 @13:08:14.68 washCycleDA #1 of 3 @13:11:10.33 recoverPSRreagent08 @13:12:20.72 washCycleDA #2 of 3 @13:15:15.61 recoverPSRreagent08 @13:16:26.00 washCycleDA #3 of 3 @13:19:20.89 recoverPSRreagent08 @13:20:31.28 Mixing DA Substrates @13:21:42.02 recoverPSRreagent08 @13:22:52.41 Processing Puck Heater at 28C before imaging Imaging da puck in Processing Clamp while storing it to tube 4 @13:23:01.51 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @13:23:18.36 Lights, camera, ACTION! Simulating PuckCamera: snap -bin=2,2 -auto /var/log/esp/da18sep2113h1000ml.tif @13:23:38.36 Waiting >20 seconds for da to relinquish FlushPuck @13:25:08.36 Simulating PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da18sep2113h1000ml15s.tif @13:25:33.36 Simulating PuckCamera: snap -bin=2,2 30.0 /var/log/esp/midres/da18sep2113h1000ml30s.tif @13:26:13.36 Simulating PuckCamera: snap -bin=1,1 70.0 /var/log/esp/hires/da18sep2113h1000ml70s.tif @13:27:34.52 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @13:27:37.37 Loading FlushPuck into Processing Clamp from FlushPuck::Garage Starting finalPurge @13:27:57.37 Waiting >20 seconds for psFlush to relinquish FlushPuck @13:36:55.72 Loading FlushPuck into Collection Clamp from Processing Clamp @13:37:13.56 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @13:37:58.08 Flushing lower manifold's waste1 port @13:38:22.13 Flushing lower manifold's waste2 port @13:38:46.18 Disinfecting external Intake with kill @13:40:19.67 Garaging FlushPuck in Collection Clamp @13:40:32.77 Completed [/home/esp/esp2/mission/2018_08_31_niagara_mission.rb:144] begun 11:30:08 Preparing to sleep until 7:22AM 9/23/2018 Asleep til 7:22AM 9/23/2018 Continuing at 07:22EDT23-Sep-18 @13:40:33.07 Shutdown @07:22:11.40EDT23-Sep-18 Phase [Sample.deep; initialPurge; da withoutWCR] @07:22:12.90 Starting initialPurge Loading FlushPuck into Collection Clamp from FlushPuck::Garage @07:22:29.10 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @07:23:14.74 Purging external Intake @07:23:38.76 Flushing upper manifold @07:24:55.56 Garaging FlushPuck in Collection Clamp @07:25:08.66 da sampling at most 1000ml Loading sh1 puck from tube 5 into Collection Clamp @07:25:32.98 collectSample 1000ml w/bubble point of 24psi Priming 7ml @07:25:33.04 Calibrating pressure and temperature sensors @07:25:46.43 Waiting for external sampler to finish priming... @07:28:47.07 Sampling 1000.0ml @07:54:06.59 Sampled 1000.0ml Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @07:54:54.15 BubblePoint: Intake @14.7psia, Outlet @14.7psia @07:55:14.47 Evacuated: Intake @14.7psia, Outlet @14.7psia @07:55:42.52 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @07:56:28.59 BubblePoint: Intake @14.7psia, Outlet @14.7psia @07:56:48.91 Evacuated: Intake @14.7psia, Outlet @14.7psia @07:57:16.96 Lysing: Adding 2.0ml AqMeOH @07:58:24.79 Heating sh1 puck from tube 5 in Collection Clamp to 70C for 10:00 @08:09:50.79 Collection Puck Heater at 70C after lysing @08:10:32.15 Recovering Lysate into Collection syringe (recycling once) @08:11:13.33 Recycling Lysate (#1 of 1) @08:12:21.56 Recovering Lysate (#2 of 2) @08:13:02.74 Lysing Complete Storing sh1 puck in Collection Clamp to tube 4 @08:13:11.51 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:13:25.62 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:13:28.38 Tranferring DA Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:17:05.46 Garaging FlushPuck in Collection Clamp @08:17:18.56 Loading lyfil puck from tube 5 into Collection Clamp @08:19:47.29 Storing lyfil puck in Collection Clamp to tube 4 @08:19:55.97 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:20:10.08 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:21:12.62 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @08:22:31.18 Garaging FlushPuck in Processing Clamp @08:22:43.03 Loading da puck from tube 5 into Processing Clamp @08:23:02.45 Heating da puck from tube 5 in Processing Clamp to 28C for 2:00:00 @08:30:41.98 recoverPSRreagent08 @08:32:29.18 addPMLReagent08 @08:33:11.25 Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:36:47.82 Garaging FlushPuck in Collection Clamp @08:41:11.25 recoverPRVreagent08 @08:42:11.52 washCycleDA #1 of 2 @08:45:07.16 recoverPSRreagent08 @08:46:17.55 washCycleDA #2 of 2 @08:49:12.44 recoverPSRreagent08 @08:50:22.83 getPMLReagent08 @08:50:45.07 addPMLReagent08 @08:59:26.64 recoverPRVreagent08 @09:00:26.15 washCycleDA #1 of 3 @09:03:21.80 recoverPSRreagent08 @09:04:32.19 washCycleDA #2 of 3 @09:07:27.08 recoverPSRreagent08 @09:08:37.47 washCycleDA #3 of 3 @09:11:32.36 recoverPSRreagent08 @09:12:42.75 Mixing DA Substrates @09:13:53.49 recoverPSRreagent08 @09:15:03.88 Processing Puck Heater at 28C before imaging Imaging da puck in Processing Clamp while storing it to tube 4 @09:15:12.70 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @09:15:29.55 Lights, camera, ACTION! Simulating PuckCamera: snap -bin=2,2 -auto /var/log/esp/da18sep2309h1000ml.tif @09:15:49.55 Waiting >20 seconds for da to relinquish FlushPuck @09:17:19.55 Simulating PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da18sep2309h1000ml15s.tif @09:17:44.55 Simulating PuckCamera: snap -bin=2,2 30.0 /var/log/esp/midres/da18sep2309h1000ml30s.tif @09:18:24.55 Simulating PuckCamera: snap -bin=1,1 70.0 /var/log/esp/hires/da18sep2309h1000ml70s.tif @09:19:45.71 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @09:19:48.27 Loading FlushPuck into Processing Clamp from FlushPuck::Garage Starting finalPurge @09:20:08.27 Waiting >20 seconds for psFlush to relinquish FlushPuck @09:29:06.61 Loading FlushPuck into Collection Clamp from Processing Clamp @09:29:24.45 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @09:30:08.97 Flushing lower manifold's waste1 port @09:30:33.02 Flushing lower manifold's waste2 port @09:30:57.07 Disinfecting external Intake with kill @09:32:30.56 Garaging FlushPuck in Collection Clamp @09:32:43.66 Completed [Sample.deep; initialPurge; da withoutWCR] begun 07:22:11 Preparing to sleep until 11:30AM 9/23/2018 Asleep til 11:30AM 9/23/2018 Continuing at 11:30 @09:32:43.96 Shutdown @11:30:11.40 Phase [/home/esp/esp2/mission/2018_08_31_niagara_mission.rb:153] @11:30:12.90 Starting initialPurge Loading FlushPuck into Collection Clamp from FlushPuck::Garage @11:30:29.10 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @11:31:14.74 Purging external Intake @11:31:38.76 Flushing upper manifold @11:32:55.56 Garaging FlushPuck in Collection Clamp @11:33:08.66 da sampling at most 1000ml Loading sh1 puck from tube 5 into Collection Clamp @11:33:33.27 collectSample 1000ml w/bubble point of 24psi Priming 7ml @11:33:33.34 Calibrating pressure and temperature sensors @11:33:46.73 Waiting for external sampler to finish priming... @11:36:47.36 Sampling 1000.0ml @12:02:06.88 Sampled 1000.0ml Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @12:02:54.45 BubblePoint: Intake @14.7psia, Outlet @14.7psia @12:03:14.76 Evacuated: Intake @14.7psia, Outlet @14.7psia @12:03:42.82 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @12:04:28.89 BubblePoint: Intake @14.7psia, Outlet @14.7psia @12:04:49.21 Evacuated: Intake @14.7psia, Outlet @14.7psia @12:05:17.26 Lysing: Adding 2.0ml AqMeOH @12:06:25.09 Heating sh1 puck from tube 5 in Collection Clamp to 70C for 10:00 @12:17:51.09 Collection Puck Heater at 70C after lysing @12:18:32.45 Recovering Lysate into Collection syringe (recycling once) @12:19:13.63 Recycling Lysate (#1 of 1) @12:20:21.85 Recovering Lysate (#2 of 2) @12:21:03.03 Lysing Complete Storing sh1 puck in Collection Clamp to tube 4 @12:21:11.51 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:21:25.62 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:21:28.08 Tranferring DA Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:25:05.16 Garaging FlushPuck in Collection Clamp @12:25:18.26 Loading lyfil puck from tube 5 into Collection Clamp @12:27:47.29 Storing lyfil puck in Collection Clamp to tube 4 @12:27:55.67 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:28:09.78 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:29:12.02 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @12:30:30.59 Garaging FlushPuck in Processing Clamp @12:30:42.44 Loading da puck from tube 5 into Processing Clamp @12:31:02.16 Heating da puck from tube 5 in Processing Clamp to 28C for 2:00:00 @12:38:41.69 recoverPSRreagent08 @12:40:28.89 addPMLReagent08 @12:41:10.96 Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:44:47.52 Garaging FlushPuck in Collection Clamp @12:49:10.96 recoverPRVreagent08 @12:50:11.22 washCycleDA #1 of 2 @12:53:06.86 recoverPSRreagent08 @12:54:17.25 washCycleDA #2 of 2 @12:57:12.15 recoverPSRreagent08 @12:58:22.54 getPMLReagent08 @12:58:44.77 addPMLReagent08 @13:07:26.35 recoverPRVreagent08 @13:08:25.85 washCycleDA #1 of 3 @13:11:21.50 recoverPSRreagent08 @13:12:31.89 washCycleDA #2 of 3 @13:15:26.78 recoverPSRreagent08 @13:16:37.17 washCycleDA #3 of 3 @13:19:32.06 recoverPSRreagent08 @13:20:42.45 Mixing DA Substrates @13:21:53.19 recoverPSRreagent08 @13:23:03.58 Processing Puck Heater at 28C before imaging Imaging da puck in Processing Clamp while storing it to tube 4 @13:23:12.11 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @13:23:28.96 Lights, camera, ACTION! Simulating PuckCamera: snap -bin=2,2 -auto /var/log/esp/da18sep2313h1000ml.tif @13:23:48.96 Waiting >20 seconds for da to relinquish FlushPuck @13:25:18.96 Simulating PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da18sep2313h1000ml15s.tif @13:25:43.96 Simulating PuckCamera: snap -bin=2,2 30.0 /var/log/esp/midres/da18sep2313h1000ml30s.tif @13:26:23.96 Simulating PuckCamera: snap -bin=1,1 70.0 /var/log/esp/hires/da18sep2313h1000ml70s.tif @13:27:45.12 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @13:27:47.45 Starting finalPurge Loading FlushPuck into Processing Clamp from FlushPuck::Garage @13:28:07.45 Waiting >20 seconds for psFlush to relinquish FlushPuck @13:37:05.80 Loading FlushPuck into Collection Clamp from Processing Clamp @13:37:23.64 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @13:38:08.16 Flushing lower manifold's waste1 port @13:38:32.21 Flushing lower manifold's waste2 port @13:38:56.26 Disinfecting external Intake with kill @13:40:29.75 Garaging FlushPuck in Collection Clamp @13:40:42.85 Completed [/home/esp/esp2/mission/2018_08_31_niagara_mission.rb:153] begun 11:30:11 Preparing to sleep until 7:24AM 9/25/2018 Asleep til 7:24AM 9/25/2018 Continuing at 07:24EDT25-Sep-18 @13:40:43.15 Shutdown @07:24:11.40EDT25-Sep-18 Phase [Sample.deep; initialPurge; da withoutWCR] @07:24:12.90 Starting initialPurge Loading FlushPuck into Collection Clamp from FlushPuck::Garage @07:24:29.10 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @07:25:14.74 Purging external Intake @07:25:38.76 Flushing upper manifold @07:26:55.56 Garaging FlushPuck in Collection Clamp @07:27:08.66 da sampling at most 1000ml Loading sh1 puck from tube 5 into Collection Clamp @07:27:33.57 collectSample 1000ml w/bubble point of 24psi Priming 7ml @07:27:33.64 Calibrating pressure and temperature sensors @07:27:47.02 Waiting for external sampler to finish priming... @07:30:47.66 Sampling 1000.0ml @07:56:07.18 Sampled 1000.0ml Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @07:56:54.74 BubblePoint: Intake @14.7psia, Outlet @14.7psia @07:57:15.06 Evacuated: Intake @14.7psia, Outlet @14.7psia @07:57:43.11 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @07:58:29.19 BubblePoint: Intake @14.7psia, Outlet @14.7psia @07:58:49.50 Evacuated: Intake @14.7psia, Outlet @14.7psia @07:59:17.56 Lysing: Adding 2.0ml AqMeOH @08:00:25.38 Heating sh1 puck from tube 5 in Collection Clamp to 70C for 10:00 @08:11:51.38 Collection Puck Heater at 70C after lysing @08:12:32.75 Recovering Lysate into Collection syringe (recycling once) @08:13:13.93 Recycling Lysate (#1 of 1) @08:14:22.15 Recovering Lysate (#2 of 2) @08:15:03.33 Lysing Complete Storing sh1 puck in Collection Clamp to tube 4 @08:15:11.51 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:15:25.62 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:15:27.96 Tranferring DA Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:19:05.04 Garaging FlushPuck in Collection Clamp @08:19:18.14 Loading lyfil puck from tube 5 into Collection Clamp @08:21:47.46 Storing lyfil puck in Collection Clamp to tube 4 @08:21:55.54 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:22:09.66 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:23:11.88 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @08:24:30.45 Garaging FlushPuck in Processing Clamp @08:24:42.30 Loading da puck from tube 5 into Processing Clamp @08:25:02.31 Heating da puck from tube 5 in Processing Clamp to 28C for 2:00:00 @08:32:41.84 recoverPSRreagent08 @08:34:29.04 addPMLReagent08 @08:35:11.11 Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:38:47.68 Garaging FlushPuck in Collection Clamp @08:43:11.11 recoverPRVreagent08 @08:44:11.38 washCycleDA #1 of 2 @08:47:07.02 recoverPSRreagent08 @08:48:17.41 washCycleDA #2 of 2 @08:51:12.30 recoverPSRreagent08 @08:52:22.69 getPMLReagent08 @08:52:44.93 addPMLReagent08 @09:01:26.50 recoverPRVreagent08 @09:02:26.01 washCycleDA #1 of 3 @09:05:21.65 recoverPSRreagent08 @09:06:32.04 washCycleDA #2 of 3 @09:09:26.94 recoverPSRreagent08 @09:10:37.33 washCycleDA #3 of 3 @09:13:32.22 recoverPSRreagent08 @09:14:42.61 Mixing DA Substrates @09:15:53.35 recoverPSRreagent08 @09:17:03.74 Processing Puck Heater at 28C before imaging Imaging da puck in Processing Clamp while storing it to tube 4 @09:17:11.97 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @09:17:28.82 Lights, camera, ACTION! Simulating PuckCamera: snap -bin=2,2 -auto /var/log/esp/da18sep2509h1000ml.tif @09:17:48.82 Waiting >20 seconds for da to relinquish FlushPuck @09:19:18.82 Simulating PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da18sep2509h1000ml15s.tif @09:19:43.82 Simulating PuckCamera: snap -bin=2,2 30.0 /var/log/esp/midres/da18sep2509h1000ml30s.tif @09:20:23.82 Simulating PuckCamera: snap -bin=1,1 70.0 /var/log/esp/hires/da18sep2509h1000ml70s.tif @09:21:44.97 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @09:21:47.31 Loading FlushPuck into Processing Clamp from FlushPuck::Garage Starting finalPurge @09:22:07.31 Waiting >20 seconds for psFlush to relinquish FlushPuck @09:31:05.66 Loading FlushPuck into Collection Clamp from Processing Clamp @09:31:23.50 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @09:32:08.02 Flushing lower manifold's waste1 port @09:32:32.07 Flushing lower manifold's waste2 port @09:32:56.12 Disinfecting external Intake with kill @09:34:29.61 Garaging FlushPuck in Collection Clamp @09:34:42.71 Completed [Sample.deep; initialPurge; da withoutWCR] begun 07:24:11 Preparing to sleep until 11:30AM 9/25/2018 Asleep til 11:30AM 9/25/2018 Continuing at 11:30 @09:34:43.01 Shutdown @11:30:11.40 Phase [/home/esp/esp2/mission/2018_08_31_niagara_mission.rb:162] @11:30:12.90 Starting initialPurge Loading FlushPuck into Collection Clamp from FlushPuck::Garage @11:30:29.10 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @11:31:14.74 Purging external Intake @11:31:38.76 Flushing upper manifold @11:32:55.56 Garaging FlushPuck in Collection Clamp @11:33:08.66 da sampling at most 1000ml Loading sh1 puck from tube 5 into Collection Clamp @11:33:33.87 collectSample 1000ml w/bubble point of 24psi Priming 7ml @11:33:33.93 Calibrating pressure and temperature sensors @11:33:47.32 Waiting for external sampler to finish priming... @11:36:47.96 Sampling 1000.0ml @12:02:07.48 Sampled 1000.0ml Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @12:02:55.04 BubblePoint: Intake @14.7psia, Outlet @14.7psia @12:03:15.36 Evacuated: Intake @14.7psia, Outlet @14.7psia @12:03:43.41 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @12:04:29.48 BubblePoint: Intake @14.7psia, Outlet @14.7psia @12:04:49.80 Evacuated: Intake @14.7psia, Outlet @14.7psia @12:05:17.85 Lysing: Adding 2.0ml AqMeOH @12:06:25.68 Heating sh1 puck from tube 5 in Collection Clamp to 70C for 10:00 @12:17:51.68 Collection Puck Heater at 70C after lysing @12:18:33.04 Recovering Lysate into Collection syringe (recycling once) @12:19:14.22 Recycling Lysate (#1 of 1) @12:20:22.45 Recovering Lysate (#2 of 2) @12:21:03.63 Lysing Complete Storing sh1 puck in Collection Clamp to tube 4 @12:21:11.51 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:21:25.62 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:21:27.96 Tranferring DA Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:25:05.04 Garaging FlushPuck in Collection Clamp @12:25:18.14 Loading lyfil puck from tube 5 into Collection Clamp @12:27:47.76 Storing lyfil puck in Collection Clamp to tube 4 @12:27:55.54 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:28:09.66 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:29:11.88 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @12:30:30.45 Garaging FlushPuck in Processing Clamp @12:30:42.30 Loading da puck from tube 5 into Processing Clamp @12:31:02.61 Heating da puck from tube 5 in Processing Clamp to 28C for 2:00:00 @12:38:42.14 recoverPSRreagent08 @12:40:29.34 addPMLReagent08 @12:41:11.41 Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:44:47.98 Garaging FlushPuck in Collection Clamp @12:49:11.41 recoverPRVreagent08 @12:50:11.67 washCycleDA #1 of 2 @12:53:07.32 recoverPSRreagent08 @12:54:17.71 washCycleDA #2 of 2 @12:57:12.60 recoverPSRreagent08 @12:58:22.99 getPMLReagent08 @12:58:45.23 addPMLReagent08 @13:07:26.80 recoverPRVreagent08 @13:08:26.31 washCycleDA #1 of 3 @13:11:21.95 recoverPSRreagent08 @13:12:32.34 washCycleDA #2 of 3 @13:15:27.23 recoverPSRreagent08 @13:16:37.62 washCycleDA #3 of 3 @13:19:32.52 recoverPSRreagent08 @13:20:42.91 Mixing DA Substrates @13:21:53.65 recoverPSRreagent08 @13:23:04.04 Processing Puck Heater at 28C before imaging Imaging da puck in Processing Clamp while storing it to tube 4 @13:23:11.97 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @13:23:28.82 Lights, camera, ACTION! Simulating PuckCamera: snap -bin=2,2 -auto /var/log/esp/da18sep2513h1000ml.tif @13:23:48.82 Waiting >20 seconds for da to relinquish FlushPuck @13:25:18.82 Simulating PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da18sep2513h1000ml15s.tif @13:25:43.82 Simulating PuckCamera: snap -bin=2,2 30.0 /var/log/esp/midres/da18sep2513h1000ml30s.tif @13:26:23.82 Simulating PuckCamera: snap -bin=1,1 70.0 /var/log/esp/hires/da18sep2513h1000ml70s.tif @13:27:44.97 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @13:27:47.31 Loading FlushPuck into Processing Clamp from FlushPuck::Garage Starting finalPurge @13:28:07.31 Waiting >20 seconds for psFlush to relinquish FlushPuck @13:37:05.66 Loading FlushPuck into Collection Clamp from Processing Clamp @13:37:23.50 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @13:38:08.02 Flushing lower manifold's waste1 port @13:38:32.07 Flushing lower manifold's waste2 port @13:38:56.12 Disinfecting external Intake with kill @13:40:29.61 Garaging FlushPuck in Collection Clamp @13:40:42.71 Completed [/home/esp/esp2/mission/2018_08_31_niagara_mission.rb:162] begun 11:30:11 Preparing to sleep until 7:26AM 9/27/2018 Asleep til 7:26AM 9/27/2018 Continuing at 07:26EDT27-Sep-18 @13:40:43.01 Shutdown @07:26:11.40EDT27-Sep-18 Phase [Sample.deep; initialPurge; da withoutWCR] @07:26:12.90 Starting initialPurge Loading FlushPuck into Collection Clamp from FlushPuck::Garage @07:26:29.10 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @07:27:14.74 Purging external Intake @07:27:38.76 Flushing upper manifold @07:28:55.56 Garaging FlushPuck in Collection Clamp @07:29:08.66 da sampling at most 1000ml Loading sh1 puck from tube 5 into Collection Clamp @07:29:34.16 collectSample 1000ml w/bubble point of 24psi Priming 7ml @07:29:34.23 Calibrating pressure and temperature sensors @07:29:47.62 Waiting for external sampler to finish priming... @07:32:48.25 Sampling 1000.0ml @07:58:07.77 Sampled 1000.0ml Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @07:58:55.34 BubblePoint: Intake @14.7psia, Outlet @14.7psia @07:59:15.65 Evacuated: Intake @14.7psia, Outlet @14.7psia @07:59:43.71 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @08:00:29.78 BubblePoint: Intake @14.7psia, Outlet @14.7psia @08:00:50.10 Evacuated: Intake @14.7psia, Outlet @14.7psia @08:01:18.15 Lysing: Adding 2.0ml AqMeOH @08:02:25.98 Heating sh1 puck from tube 5 in Collection Clamp to 70C for 10:00 @08:13:51.98 Collection Puck Heater at 70C after lysing @08:14:33.34 Recovering Lysate into Collection syringe (recycling once) @08:15:14.52 Recycling Lysate (#1 of 1) @08:16:22.74 Recovering Lysate (#2 of 2) @08:17:03.92 Lysing Complete Storing sh1 puck in Collection Clamp to tube 4 @08:17:11.51 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:17:25.62 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:17:27.96 Tranferring DA Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:21:05.04 Garaging FlushPuck in Collection Clamp @08:21:18.14 Loading lyfil puck from tube 5 into Collection Clamp @08:23:48.05 Storing lyfil puck in Collection Clamp to tube 4 @08:23:55.54 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:24:09.66 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:25:11.88 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @08:26:30.45 Garaging FlushPuck in Processing Clamp @08:26:42.30 Loading da puck from tube 5 into Processing Clamp @08:27:02.91 Heating da puck from tube 5 in Processing Clamp to 28C for 2:00:00 @08:34:42.44 recoverPSRreagent08 @08:36:29.64 addPMLReagent08 @08:37:11.71 Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:40:48.27 Garaging FlushPuck in Collection Clamp @08:45:11.71 recoverPRVreagent08 @08:46:11.97 washCycleDA #1 of 2 @08:49:07.61 recoverPSRreagent08 @08:50:18.00 washCycleDA #2 of 2 @08:53:12.90 recoverPSRreagent08 @08:54:23.29 getPMLReagent08 @08:54:45.52 addPMLReagent08 @09:03:27.10 recoverPRVreagent08 @09:04:26.60 washCycleDA #1 of 3 @09:07:22.25 recoverPSRreagent08 @09:08:32.64 washCycleDA #2 of 3 @09:11:27.53 recoverPSRreagent08 @09:12:37.92 washCycleDA #3 of 3 @09:15:32.81 recoverPSRreagent08 @09:16:43.20 Mixing DA Substrates @09:17:53.94 recoverPSRreagent08 @09:19:04.33 Processing Puck Heater at 28C before imaging Imaging da puck in Processing Clamp while storing it to tube 4 @09:19:11.97 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @09:19:28.82 Lights, camera, ACTION! Simulating PuckCamera: snap -bin=2,2 -auto /var/log/esp/da18sep2709h1000ml.tif @09:19:48.82 Waiting >20 seconds for da to relinquish FlushPuck @09:21:18.82 Simulating PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da18sep2709h1000ml15s.tif @09:21:43.82 Simulating PuckCamera: snap -bin=2,2 30.0 /var/log/esp/midres/da18sep2709h1000ml30s.tif @09:22:23.82 Simulating PuckCamera: snap -bin=1,1 70.0 /var/log/esp/hires/da18sep2709h1000ml70s.tif @09:23:44.97 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @09:23:47.31 Starting finalPurge Loading FlushPuck into Processing Clamp from FlushPuck::Garage @09:24:07.31 Waiting >20 seconds for psFlush to relinquish FlushPuck @09:33:05.66 Loading FlushPuck into Collection Clamp from Processing Clamp @09:33:23.50 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @09:34:08.02 Flushing lower manifold's waste1 port @09:34:32.07 Flushing lower manifold's waste2 port @09:34:56.12 Disinfecting external Intake with kill @09:36:29.61 Garaging FlushPuck in Collection Clamp @09:36:42.71 Completed [Sample.deep; initialPurge; da withoutWCR] begun 07:26:11 Preparing to sleep until 11:30AM 9/27/2018 Asleep til 11:30AM 9/27/2018 Continuing at 11:30 @09:36:43.01 Shutdown @11:30:11.40 Phase [/home/esp/esp2/mission/2018_08_31_niagara_mission.rb:171] @11:30:12.90 Starting initialPurge Loading FlushPuck into Collection Clamp from FlushPuck::Garage @11:30:29.10 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @11:31:14.74 Purging external Intake @11:31:38.76 Flushing upper manifold @11:32:55.56 Garaging FlushPuck in Collection Clamp @11:33:08.66 da sampling at most 1000ml Loading sh1 puck from tube 5 into Collection Clamp @11:33:34.46 collectSample 1000ml w/bubble point of 24psi Priming 7ml @11:33:34.53 Calibrating pressure and temperature sensors @11:33:47.92 Waiting for external sampler to finish priming... @11:36:48.55 Sampling 1000.0ml @12:02:08.07 Sampled 1000.0ml Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @12:02:55.63 BubblePoint: Intake @14.7psia, Outlet @14.7psia @12:03:15.95 Evacuated: Intake @14.7psia, Outlet @14.7psia @12:03:44.01 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @12:04:30.08 BubblePoint: Intake @14.7psia, Outlet @14.7psia @12:04:50.39 Evacuated: Intake @14.7psia, Outlet @14.7psia @12:05:18.45 Lysing: Adding 2.0ml AqMeOH @12:06:26.27 Heating sh1 puck from tube 5 in Collection Clamp to 70C for 10:00 @12:17:52.27 Collection Puck Heater at 70C after lysing @12:18:33.64 Recovering Lysate into Collection syringe (recycling once) @12:19:14.82 Recycling Lysate (#1 of 1) @12:20:23.04 Recovering Lysate (#2 of 2) @12:21:04.22 Lysing Complete Storing sh1 puck in Collection Clamp to tube 4 @12:21:11.51 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:21:25.62 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:21:27.96 Tranferring DA Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:25:05.04 Garaging FlushPuck in Collection Clamp @12:25:18.14 Loading lyfil puck from tube 5 into Collection Clamp @12:27:48.35 Storing lyfil puck in Collection Clamp to tube 4 @12:27:55.54 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:28:09.66 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:29:11.88 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @12:30:30.45 Garaging FlushPuck in Processing Clamp @12:30:42.30 Loading da puck from tube 5 into Processing Clamp @12:31:03.19 Heating da puck from tube 5 in Processing Clamp to 28C for 2:00:00 @12:38:42.72 recoverPSRreagent08 @12:40:29.92 addPMLReagent08 @12:41:11.99 Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:44:48.55 Garaging FlushPuck in Collection Clamp @12:49:11.99 recoverPRVreagent08 @12:50:12.25 washCycleDA #1 of 2 @12:53:07.90 recoverPSRreagent08 @12:54:18.29 washCycleDA #2 of 2 @12:57:13.18 recoverPSRreagent08 @12:58:23.57 getPMLReagent08 @12:58:45.80 addPMLReagent08 @13:07:27.38 recoverPRVreagent08 @13:08:26.88 washCycleDA #1 of 3 @13:11:22.53 recoverPSRreagent08 @13:12:32.92 washCycleDA #2 of 3 @13:15:27.81 recoverPSRreagent08 @13:16:38.20 washCycleDA #3 of 3 @13:19:33.09 recoverPSRreagent08 @13:20:43.48 Mixing DA Substrates @13:21:54.23 recoverPSRreagent08 @13:23:04.62 Processing Puck Heater at 28C before imaging Imaging da puck in Processing Clamp while storing it to tube 4 @13:23:11.95 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @13:23:28.80 Lights, camera, ACTION! Simulating PuckCamera: snap -bin=2,2 -auto /var/log/esp/da18sep2713h1000ml.tif @13:23:48.80 Waiting >20 seconds for da to relinquish FlushPuck @13:25:18.80 Simulating PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da18sep2713h1000ml15s.tif @13:25:43.80 Simulating PuckCamera: snap -bin=2,2 30.0 /var/log/esp/midres/da18sep2713h1000ml30s.tif @13:26:23.80 Simulating PuckCamera: snap -bin=1,1 70.0 /var/log/esp/hires/da18sep2713h1000ml70s.tif @13:27:44.96 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @13:27:47.30 Loading FlushPuck into Processing Clamp from FlushPuck::Garage Starting finalPurge @13:28:07.30 Waiting >20 seconds for psFlush to relinquish FlushPuck @13:37:05.64 Loading FlushPuck into Collection Clamp from Processing Clamp @13:37:23.48 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @13:38:08.00 Flushing lower manifold's waste1 port @13:38:32.05 Flushing lower manifold's waste2 port @13:38:56.10 Disinfecting external Intake with kill @13:40:29.59 Garaging FlushPuck in Collection Clamp @13:40:42.69 Completed [/home/esp/esp2/mission/2018_08_31_niagara_mission.rb:171] begun 11:30:11 Preparing to sleep until 7:28AM 9/29/2018 Asleep til 7:28AM 9/29/2018 Continuing at 07:28EDT29-Sep-18 @13:40:42.99 Shutdown @07:28:11.40EDT29-Sep-18 Phase [Sample.deep; initialPurge; da withoutWCR] @07:28:12.90 Starting initialPurge Loading FlushPuck into Collection Clamp from FlushPuck::Garage @07:28:29.10 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @07:29:14.74 Purging external Intake @07:29:38.76 Flushing upper manifold @07:30:55.56 Garaging FlushPuck in Collection Clamp @07:31:08.66 da sampling at most 1000ml Loading sh1 puck from tube 5 into Collection Clamp @07:31:34.76 collectSample 1000ml w/bubble point of 24psi Priming 7ml @07:31:34.82 Calibrating pressure and temperature sensors @07:31:48.21 Waiting for external sampler to finish priming... @07:34:48.85 Sampling 1000.0ml @08:00:08.37 Sampled 1000.0ml Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @08:00:55.93 BubblePoint: Intake @14.7psia, Outlet @14.7psia @08:01:16.25 Evacuated: Intake @14.7psia, Outlet @14.7psia @08:01:44.30 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @08:02:30.38 BubblePoint: Intake @14.7psia, Outlet @14.7psia @08:02:50.69 Evacuated: Intake @14.7psia, Outlet @14.7psia @08:03:18.75 Lysing: Adding 2.0ml AqMeOH @08:04:26.57 Heating sh1 puck from tube 5 in Collection Clamp to 70C for 10:00 @08:15:52.57 Collection Puck Heater at 70C after lysing @08:16:33.93 Recovering Lysate into Collection syringe (recycling once) @08:17:15.11 Recycling Lysate (#1 of 1) @08:18:23.34 Recovering Lysate (#2 of 2) @08:19:04.52 Lysing Complete Storing sh1 puck in Collection Clamp to tube 4 @08:19:11.51 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:19:25.62 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:19:27.96 Tranferring DA Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:23:05.04 Garaging FlushPuck in Collection Clamp @08:23:18.14 Loading lyfil puck from tube 6 into Collection Clamp @08:25:49.62 Storing lyfil puck in Collection Clamp to tube 5 @08:26:04.09 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:27:07.13 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @08:28:25.70 Garaging FlushPuck in Processing Clamp @08:28:37.55 Loading da puck from tube 6 into Processing Clamp @08:28:56.56 Heating da puck from tube 6 in Processing Clamp to 28C for 2:00:00 @08:36:36.09 recoverPSRreagent08 @08:38:23.29 addPMLReagent08 @08:39:05.36 Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:42:41.93 Garaging FlushPuck in Collection Clamp @08:47:05.36 recoverPRVreagent08 @08:48:05.63 washCycleDA #1 of 2 @08:51:01.27 recoverPSRreagent08 @08:52:11.66 washCycleDA #2 of 2 @08:55:06.55 recoverPSRreagent08 @08:56:16.94 getPMLReagent08 @08:56:39.18 addPMLReagent08 @09:05:20.75 recoverPRVreagent08 @09:06:20.26 washCycleDA #1 of 3 @09:09:15.90 recoverPSRreagent08 @09:10:26.29 washCycleDA #2 of 3 @09:13:21.19 recoverPSRreagent08 @09:14:31.58 washCycleDA #3 of 3 @09:17:26.47 recoverPSRreagent08 @09:18:36.86 Mixing DA Substrates @09:19:47.60 recoverPSRreagent08 @09:20:57.99 Processing Puck Heater at 28C before imaging Imaging da puck in Processing Clamp while storing it to tube 5 @09:21:07.20 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @09:21:24.05 Lights, camera, ACTION! Simulating PuckCamera: snap -bin=2,2 -auto /var/log/esp/da18sep2909h1000ml.tif @09:21:44.05 Waiting >20 seconds for da to relinquish FlushPuck @09:23:14.05 Simulating PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da18sep2909h1000ml15s.tif @09:23:39.05 Simulating PuckCamera: snap -bin=2,2 30.0 /var/log/esp/midres/da18sep2909h1000ml30s.tif @09:24:19.05 Simulating PuckCamera: snap -bin=1,1 70.0 /var/log/esp/hires/da18sep2909h1000ml70s.tif @09:25:40.21 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @09:25:43.16 Starting finalPurge Loading FlushPuck into Processing Clamp from FlushPuck::Garage @09:26:03.16 Waiting >20 seconds for psFlush to relinquish FlushPuck @09:35:01.50 Loading FlushPuck into Collection Clamp from Processing Clamp @09:35:19.34 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @09:36:03.86 Flushing lower manifold's waste1 port @09:36:27.91 Flushing lower manifold's waste2 port @09:36:51.96 Disinfecting external Intake with kill @09:38:25.45 Garaging FlushPuck in Collection Clamp @09:38:38.55 Completed [Sample.deep; initialPurge; da withoutWCR] begun 07:28:11 Preparing to sleep until 11:30AM 9/29/2018 Asleep til 11:30AM 9/29/2018 Continuing at 11:30 @09:38:38.85 Shutdown @11:30:14.54 Phase [/home/esp/esp2/mission/2018_08_31_niagara_mission.rb:180] @11:30:16.04 Starting initialPurge Loading FlushPuck into Collection Clamp from FlushPuck::Garage @11:30:32.24 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @11:31:17.88 Purging external Intake @11:31:41.90 Flushing upper manifold @11:32:58.70 Garaging FlushPuck in Collection Clamp @11:33:11.80 da sampling at most 1000ml Loading sh1 puck from tube 6 into Collection Clamp @11:33:39.18 collectSample 1000ml w/bubble point of 24psi Priming 7ml @11:33:39.24 Calibrating pressure and temperature sensors @11:33:52.63 Waiting for external sampler to finish priming... @11:36:53.27 Sampling 1000.0ml @12:02:12.79 Sampled 1000.0ml Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @12:03:00.35 BubblePoint: Intake @14.7psia, Outlet @14.7psia @12:03:20.67 Evacuated: Intake @14.7psia, Outlet @14.7psia @12:03:48.72 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @12:04:34.80 BubblePoint: Intake @14.7psia, Outlet @14.7psia @12:04:55.11 Evacuated: Intake @14.7psia, Outlet @14.7psia @12:05:23.17 Lysing: Adding 2.0ml AqMeOH @12:06:30.99 Heating sh1 puck from tube 6 in Collection Clamp to 70C for 10:00 @12:17:56.99 Collection Puck Heater at 70C after lysing @12:18:38.36 Recovering Lysate into Collection syringe (recycling once) @12:19:19.54 Recycling Lysate (#1 of 1) @12:20:27.76 Recovering Lysate (#2 of 2) @12:21:08.94 Lysing Complete Storing sh1 puck in Collection Clamp to tube 5 @12:21:17.80 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:21:31.92 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:21:34.77 Tranferring DA Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:25:11.85 Garaging FlushPuck in Collection Clamp @12:25:24.95 Loading lyfil puck from tube 6 into Collection Clamp @12:27:53.58 Storing lyfil puck in Collection Clamp to tube 5 @12:28:02.36 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:28:16.47 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:29:19.12 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @12:30:37.68 Garaging FlushPuck in Processing Clamp @12:30:49.53 Loading da puck from tube 6 into Processing Clamp @12:31:08.85 Heating da puck from tube 6 in Processing Clamp to 28C for 2:00:00 @12:38:48.37 recoverPSRreagent08 @12:40:35.57 addPMLReagent08 @12:41:17.65 Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:44:54.21 Garaging FlushPuck in Collection Clamp @12:49:17.65 recoverPRVreagent08 @12:50:17.91 washCycleDA #1 of 2 @12:53:13.55 recoverPSRreagent08 @12:54:23.94 washCycleDA #2 of 2 @12:57:18.83 recoverPSRreagent08 @12:58:29.22 getPMLReagent08 @12:58:51.46 addPMLReagent08 @13:07:33.03 recoverPRVreagent08 @13:08:32.54 washCycleDA #1 of 3 @13:11:28.19 recoverPSRreagent08 @13:12:38.58 washCycleDA #2 of 3 @13:15:33.47 recoverPSRreagent08 @13:16:43.86 washCycleDA #3 of 3 @13:19:38.75 recoverPSRreagent08 @13:20:49.14 Mixing DA Substrates @13:21:59.88 recoverPSRreagent08 @13:23:10.27 Processing Puck Heater at 28C before imaging Imaging da puck in Processing Clamp while storing it to tube 5 @13:23:19.18 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @13:23:36.03 Lights, camera, ACTION! Simulating PuckCamera: snap -bin=2,2 -auto /var/log/esp/da18sep2913h1000ml.tif @13:23:56.03 Waiting >20 seconds for da to relinquish FlushPuck @13:25:26.03 Simulating PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da18sep2913h1000ml15s.tif @13:25:51.03 Simulating PuckCamera: snap -bin=2,2 30.0 /var/log/esp/midres/da18sep2913h1000ml30s.tif @13:26:31.03 Simulating PuckCamera: snap -bin=1,1 70.0 /var/log/esp/hires/da18sep2913h1000ml70s.tif @13:27:52.19 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @13:27:54.84 Starting finalPurge Loading FlushPuck into Processing Clamp from FlushPuck::Garage @13:28:14.84 Waiting >20 seconds for psFlush to relinquish FlushPuck @13:37:13.19 Loading FlushPuck into Collection Clamp from Processing Clamp @13:37:31.03 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @13:38:15.55 Flushing lower manifold's waste1 port @13:38:39.60 Flushing lower manifold's waste2 port @13:39:03.65 Disinfecting external Intake with kill @13:40:37.14 Garaging FlushPuck in Collection Clamp @13:40:50.24 Completed [/home/esp/esp2/mission/2018_08_31_niagara_mission.rb:180] begun 11:30:14 Preparing to sleep until 7:31AM 10/01/2018 Asleep til 7:31AM 10/01/2018 Continuing at 07:31EDT01-Oct-18 @13:40:50.54 Shutdown @07:31:14.54EDT01-Oct-18 Phase [Sample.deep; initialPurge; da withoutWCR] @07:31:16.04 Starting initialPurge Loading FlushPuck into Collection Clamp from FlushPuck::Garage @07:31:32.24 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @07:32:17.88 Purging external Intake @07:32:41.90 Flushing upper manifold @07:33:58.70 Garaging FlushPuck in Collection Clamp @07:34:11.80 da sampling at most 1000ml Loading sh1 puck from tube 6 into Collection Clamp @07:34:39.48 collectSample 1000ml w/bubble point of 24psi Priming 7ml @07:34:39.54 Calibrating pressure and temperature sensors @07:34:52.93 Waiting for external sampler to finish priming... @07:37:53.57 Sampling 1000.0ml @08:03:13.09 Sampled 1000.0ml Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @08:04:00.65 BubblePoint: Intake @14.7psia, Outlet @14.7psia @08:04:20.97 Evacuated: Intake @14.7psia, Outlet @14.7psia @08:04:49.02 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @08:05:35.09 BubblePoint: Intake @14.7psia, Outlet @14.7psia @08:05:55.41 Evacuated: Intake @14.7psia, Outlet @14.7psia @08:06:23.46 Lysing: Adding 2.0ml AqMeOH @08:07:31.29 Heating sh1 puck from tube 6 in Collection Clamp to 70C for 10:00 @08:18:57.29 Collection Puck Heater at 70C after lysing @08:19:38.65 Recovering Lysate into Collection syringe (recycling once) @08:20:19.83 Recycling Lysate (#1 of 1) @08:21:28.06 Recovering Lysate (#2 of 2) @08:22:09.24 Lysing Complete Storing sh1 puck in Collection Clamp to tube 5 @08:22:17.82 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:22:31.93 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:22:34.49 Tranferring DA Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:26:11.57 Garaging FlushPuck in Collection Clamp @08:26:24.67 Loading lyfil puck from tube 6 into Collection Clamp @08:28:53.60 Storing lyfil puck in Collection Clamp to tube 5 @08:29:02.08 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:29:16.19 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:30:18.54 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @08:31:37.10 Garaging FlushPuck in Processing Clamp @08:31:48.95 Loading da puck from tube 6 into Processing Clamp @08:32:08.56 Heating da puck from tube 6 in Processing Clamp to 28C for 2:00:00 @08:39:48.09 recoverPSRreagent08 @08:41:35.29 addPMLReagent08 @08:42:17.36 Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:45:53.93 Garaging FlushPuck in Collection Clamp @08:50:17.36 recoverPRVreagent08 @08:51:17.63 washCycleDA #1 of 2 @08:54:13.27 recoverPSRreagent08 @08:55:23.66 washCycleDA #2 of 2 @08:58:18.55 recoverPSRreagent08 @08:59:28.94 getPMLReagent08 @08:59:51.18 addPMLReagent08 @09:08:32.75 recoverPRVreagent08 @09:09:32.26 washCycleDA #1 of 3 @09:12:27.90 recoverPSRreagent08 @09:13:38.29 washCycleDA #2 of 3 @09:16:33.19 recoverPSRreagent08 @09:17:43.58 washCycleDA #3 of 3 @09:20:38.47 recoverPSRreagent08 @09:21:48.86 Mixing DA Substrates @09:22:59.60 recoverPSRreagent08 @09:24:09.99 Processing Puck Heater at 28C before imaging Imaging da puck in Processing Clamp while storing it to tube 5 @09:24:18.61 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @09:24:35.46 Lights, camera, ACTION! Simulating PuckCamera: snap -bin=2,2 -auto /var/log/esp/da18oct0109h1000ml.tif @09:24:55.46 Waiting >20 seconds for da to relinquish FlushPuck @09:26:25.46 Simulating PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da18oct0109h1000ml15s.tif @09:26:50.46 Simulating PuckCamera: snap -bin=2,2 30.0 /var/log/esp/midres/da18oct0109h1000ml30s.tif @09:27:30.46 Simulating PuckCamera: snap -bin=1,1 70.0 /var/log/esp/hires/da18oct0109h1000ml70s.tif @09:28:51.62 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @09:28:53.97 Loading FlushPuck into Processing Clamp from FlushPuck::Garage Starting finalPurge @09:29:13.97 Waiting >20 seconds for psFlush to relinquish FlushPuck @09:38:12.31 Loading FlushPuck into Collection Clamp from Processing Clamp @09:38:30.16 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @09:39:14.67 Flushing lower manifold's waste1 port @09:39:38.73 Flushing lower manifold's waste2 port @09:40:02.78 Disinfecting external Intake with kill @09:41:36.26 Garaging FlushPuck in Collection Clamp @09:41:49.36 Completed [Sample.deep; initialPurge; da withoutWCR] begun 07:31:14 Preparing to sleep until 11:30AM 10/01/2018 Asleep til 11:30AM 10/01/2018 Continuing at 11:30 @09:41:49.66 Shutdown @11:30:14.54 Phase [/home/esp/esp2/mission/2018_08_31_niagara_mission.rb:189] @11:30:16.04 Starting initialPurge Loading FlushPuck into Collection Clamp from FlushPuck::Garage @11:30:32.24 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @11:31:17.88 Purging external Intake @11:31:41.90 Flushing upper manifold @11:32:58.70 Garaging FlushPuck in Collection Clamp @11:33:11.80 da sampling at most 1000ml Loading sh1 puck from tube 6 into Collection Clamp @11:33:39.77 collectSample 1000ml w/bubble point of 24psi Priming 7ml @11:33:39.84 Calibrating pressure and temperature sensors @11:33:53.23 Waiting for external sampler to finish priming... @11:36:53.86 Sampling 1000.0ml @12:02:13.38 Sampled 1000.0ml Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @12:03:00.95 BubblePoint: Intake @14.7psia, Outlet @14.7psia @12:03:21.26 Evacuated: Intake @14.7psia, Outlet @14.7psia @12:03:49.32 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @12:04:35.39 BubblePoint: Intake @14.7psia, Outlet @14.7psia @12:04:55.71 Evacuated: Intake @14.7psia, Outlet @14.7psia @12:05:23.76 Lysing: Adding 2.0ml AqMeOH @12:06:31.59 Heating sh1 puck from tube 6 in Collection Clamp to 70C for 10:00 @12:17:57.59 Collection Puck Heater at 70C after lysing @12:18:38.95 Recovering Lysate into Collection syringe (recycling once) @12:19:20.13 Recycling Lysate (#1 of 1) @12:20:28.35 Recovering Lysate (#2 of 2) @12:21:09.53 Lysing Complete Storing sh1 puck in Collection Clamp to tube 5 @12:21:17.82 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:21:31.93 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:21:34.27 Tranferring DA Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:25:11.35 Garaging FlushPuck in Collection Clamp @12:25:24.45 Loading lyfil puck from tube 6 into Collection Clamp @12:27:53.68 Storing lyfil puck in Collection Clamp to tube 5 @12:28:01.86 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:28:15.97 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:29:18.20 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @12:30:36.76 Garaging FlushPuck in Processing Clamp @12:30:48.61 Loading da puck from tube 6 into Processing Clamp @12:31:08.52 Heating da puck from tube 6 in Processing Clamp to 28C for 2:00:00 @12:38:48.04 recoverPSRreagent08 @12:40:35.25 addPMLReagent08 @12:41:17.32 Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:44:53.88 Garaging FlushPuck in Collection Clamp @12:49:17.32 recoverPRVreagent08 @12:50:17.58 washCycleDA #1 of 2 @12:53:13.22 recoverPSRreagent08 @12:54:23.61 washCycleDA #2 of 2 @12:57:18.51 recoverPSRreagent08 @12:58:28.90 getPMLReagent08 @12:58:51.13 addPMLReagent08 @13:07:32.70 recoverPRVreagent08 @13:08:32.21 washCycleDA #1 of 3 @13:11:27.86 recoverPSRreagent08 @13:12:38.25 washCycleDA #2 of 3 @13:15:33.14 recoverPSRreagent08 @13:16:43.53 washCycleDA #3 of 3 @13:19:38.42 recoverPSRreagent08 @13:20:48.81 Mixing DA Substrates @13:21:59.55 recoverPSRreagent08 @13:23:09.94 Processing Puck Heater at 28C before imaging Imaging da puck in Processing Clamp while storing it to tube 5 @13:23:18.26 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @13:23:35.11 Lights, camera, ACTION! Simulating PuckCamera: snap -bin=2,2 -auto /var/log/esp/da18oct0113h1000ml.tif @13:23:55.11 Waiting >20 seconds for da to relinquish FlushPuck @13:25:25.11 Simulating PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da18oct0113h1000ml15s.tif @13:25:50.11 Simulating PuckCamera: snap -bin=2,2 30.0 /var/log/esp/midres/da18oct0113h1000ml30s.tif @13:26:30.11 Simulating PuckCamera: snap -bin=1,1 70.0 /var/log/esp/hires/da18oct0113h1000ml70s.tif @13:27:51.27 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @13:27:53.61 Loading FlushPuck into Processing Clamp from FlushPuck::Garage Starting finalPurge @13:28:13.61 Waiting >20 seconds for psFlush to relinquish FlushPuck @13:37:11.95 Loading FlushPuck into Collection Clamp from Processing Clamp @13:37:29.80 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @13:38:14.31 Flushing lower manifold's waste1 port @13:38:38.37 Flushing lower manifold's waste2 port @13:39:02.42 Disinfecting external Intake with kill @13:40:35.90 Garaging FlushPuck in Collection Clamp @13:40:49.00 Completed [/home/esp/esp2/mission/2018_08_31_niagara_mission.rb:189] begun 11:30:14 Preparing to sleep until 7:33AM 10/03/2018 Asleep til 7:33AM 10/03/2018 Continuing at 07:33EDT03-Oct-18 @13:40:49.30 Shutdown @07:33:14.54EDT03-Oct-18 Phase [Sample.deep; initialPurge; da withoutWCR] @07:33:16.04 Starting initialPurge Loading FlushPuck into Collection Clamp from FlushPuck::Garage @07:33:32.24 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @07:34:17.88 Purging external Intake @07:34:41.90 Flushing upper manifold @07:35:58.70 Garaging FlushPuck in Collection Clamp @07:36:11.80 da sampling at most 1000ml Loading sh1 puck from tube 6 into Collection Clamp @07:36:40.07 collectSample 1000ml w/bubble point of 24psi Priming 7ml @07:36:40.14 Calibrating pressure and temperature sensors @07:36:53.52 Waiting for external sampler to finish priming... @07:39:54.16 Sampling 1000.0ml @08:05:13.68 Sampled 1000.0ml Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @08:06:01.24 BubblePoint: Intake @14.7psia, Outlet @14.7psia @08:06:21.56 Evacuated: Intake @14.7psia, Outlet @14.7psia @08:06:49.61 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @08:07:35.69 BubblePoint: Intake @14.7psia, Outlet @14.7psia @08:07:56.00 Evacuated: Intake @14.7psia, Outlet @14.7psia @08:08:24.06 Lysing: Adding 2.0ml AqMeOH @08:09:31.88 Heating sh1 puck from tube 6 in Collection Clamp to 70C for 10:00 @08:20:57.88 Collection Puck Heater at 70C after lysing @08:21:39.25 Recovering Lysate into Collection syringe (recycling once) @08:22:20.43 Recycling Lysate (#1 of 1) @08:23:28.65 Recovering Lysate (#2 of 2) @08:24:09.83 Lysing Complete Storing sh1 puck in Collection Clamp to tube 5 @08:24:17.82 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:24:31.93 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:24:34.27 Tranferring DA Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:28:11.35 Garaging FlushPuck in Collection Clamp @08:28:24.45 Loading lyfil puck from tube 6 into Collection Clamp @08:30:53.98 Storing lyfil puck in Collection Clamp to tube 5 @08:31:01.86 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:31:15.97 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:32:18.20 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @08:33:36.76 Garaging FlushPuck in Processing Clamp @08:33:48.61 Loading da puck from tube 6 into Processing Clamp @08:34:08.81 Heating da puck from tube 6 in Processing Clamp to 28C for 2:00:00 @08:41:48.34 recoverPSRreagent08 @08:43:35.54 addPMLReagent08 @08:44:17.61 Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:47:54.18 Garaging FlushPuck in Collection Clamp @08:52:17.61 recoverPRVreagent08 @08:53:17.88 washCycleDA #1 of 2 @08:56:13.52 recoverPSRreagent08 @08:57:23.91 washCycleDA #2 of 2 @09:00:18.80 recoverPSRreagent08 @09:01:29.19 getPMLReagent08 @09:01:51.43 addPMLReagent08 @09:10:33.00 recoverPRVreagent08 @09:11:32.51 washCycleDA #1 of 3 @09:14:28.15 recoverPSRreagent08 @09:15:38.54 washCycleDA #2 of 3 @09:18:33.44 recoverPSRreagent08 @09:19:43.83 washCycleDA #3 of 3 @09:22:38.72 recoverPSRreagent08 @09:23:49.11 Mixing DA Substrates @09:24:59.85 recoverPSRreagent08 @09:26:10.24 Processing Puck Heater at 28C before imaging Imaging da puck in Processing Clamp while storing it to tube 5 @09:26:18.26 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @09:26:35.11 Lights, camera, ACTION! Simulating PuckCamera: snap -bin=2,2 -auto /var/log/esp/da18oct0309h1000ml.tif @09:26:55.11 Waiting >20 seconds for da to relinquish FlushPuck @09:28:25.11 Simulating PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da18oct0309h1000ml15s.tif @09:28:50.11 Simulating PuckCamera: snap -bin=2,2 30.0 /var/log/esp/midres/da18oct0309h1000ml30s.tif @09:29:30.11 Simulating PuckCamera: snap -bin=1,1 70.0 /var/log/esp/hires/da18oct0309h1000ml70s.tif @09:30:51.27 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @09:30:53.61 Starting finalPurge Loading FlushPuck into Processing Clamp from FlushPuck::Garage @09:31:13.61 Waiting >20 seconds for psFlush to relinquish FlushPuck @09:40:11.95 Loading FlushPuck into Collection Clamp from Processing Clamp @09:40:29.80 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @09:41:14.31 Flushing lower manifold's waste1 port @09:41:38.37 Flushing lower manifold's waste2 port @09:42:02.42 Disinfecting external Intake with kill @09:43:35.90 Garaging FlushPuck in Collection Clamp @09:43:49.00 Completed [Sample.deep; initialPurge; da withoutWCR] begun 07:33:14 Preparing to sleep until 11:30AM 10/03/2018 Asleep til 11:30AM 10/03/2018 Continuing at 11:30 @09:43:49.30 Shutdown @11:30:14.54 Phase [/home/esp/esp2/mission/2018_08_31_niagara_mission.rb:198] @11:30:16.04 Starting initialPurge Loading FlushPuck into Collection Clamp from FlushPuck::Garage @11:30:32.24 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @11:31:17.88 Purging external Intake @11:31:41.90 Flushing upper manifold @11:32:58.70 Garaging FlushPuck in Collection Clamp @11:33:11.80 da sampling at most 1000ml Loading sh1 puck from tube 6 into Collection Clamp @11:33:40.37 collectSample 1000ml w/bubble point of 24psi Priming 7ml @11:33:40.43 Calibrating pressure and temperature sensors @11:33:53.82 Waiting for external sampler to finish priming... @11:36:54.46 Sampling 1000.0ml @12:02:13.98 Sampled 1000.0ml Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @12:03:01.54 BubblePoint: Intake @14.7psia, Outlet @14.7psia @12:03:21.86 Evacuated: Intake @14.7psia, Outlet @14.7psia @12:03:49.91 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @12:04:35.98 BubblePoint: Intake @14.7psia, Outlet @14.7psia @12:04:56.30 Evacuated: Intake @14.7psia, Outlet @14.7psia @12:05:24.35 Lysing: Adding 2.0ml AqMeOH @12:06:32.18 Heating sh1 puck from tube 6 in Collection Clamp to 70C for 10:00 @12:17:58.18 Collection Puck Heater at 70C after lysing @12:18:39.54 Recovering Lysate into Collection syringe (recycling once) @12:19:20.72 Recycling Lysate (#1 of 1) @12:20:28.95 Recovering Lysate (#2 of 2) @12:21:10.13 Lysing Complete Storing sh1 puck in Collection Clamp to tube 5 @12:21:17.82 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:21:31.93 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:21:34.27 Tranferring DA Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:25:11.35 Garaging FlushPuck in Collection Clamp @12:25:24.45 Loading lyfil puck from tube 6 into Collection Clamp @12:27:54.27 Storing lyfil puck in Collection Clamp to tube 5 @12:28:01.86 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:28:15.97 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:29:18.20 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @12:30:36.76 Garaging FlushPuck in Processing Clamp @12:30:48.61 Loading da puck from tube 6 into Processing Clamp @12:31:09.11 Heating da puck from tube 6 in Processing Clamp to 28C for 2:00:00 @12:38:48.64 recoverPSRreagent08 @12:40:35.84 addPMLReagent08 @12:41:17.91 Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:44:54.48 Garaging FlushPuck in Collection Clamp @12:49:17.91 recoverPRVreagent08 @12:50:18.17 washCycleDA #1 of 2 @12:53:13.82 recoverPSRreagent08 @12:54:24.21 washCycleDA #2 of 2 @12:57:19.10 recoverPSRreagent08 @12:58:29.49 getPMLReagent08 @12:58:51.73 addPMLReagent08 @13:07:33.30 recoverPRVreagent08 @13:08:32.81 washCycleDA #1 of 3 @13:11:28.45 recoverPSRreagent08 @13:12:38.84 washCycleDA #2 of 3 @13:15:33.73 recoverPSRreagent08 @13:16:44.12 washCycleDA #3 of 3 @13:19:39.02 recoverPSRreagent08 @13:20:49.41 Mixing DA Substrates @13:22:00.15 recoverPSRreagent08 @13:23:10.54 Processing Puck Heater at 28C before imaging Imaging da puck in Processing Clamp while storing it to tube 5 @13:23:18.26 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @13:23:35.11 Lights, camera, ACTION! Simulating PuckCamera: snap -bin=2,2 -auto /var/log/esp/da18oct0313h1000ml.tif @13:23:55.11 Waiting >20 seconds for da to relinquish FlushPuck @13:25:25.11 Simulating PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da18oct0313h1000ml15s.tif @13:25:50.11 Simulating PuckCamera: snap -bin=2,2 30.0 /var/log/esp/midres/da18oct0313h1000ml30s.tif @13:26:30.11 Simulating PuckCamera: snap -bin=1,1 70.0 /var/log/esp/hires/da18oct0313h1000ml70s.tif @13:27:51.27 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @13:27:53.61 Loading FlushPuck into Processing Clamp from FlushPuck::Garage Starting finalPurge @13:28:13.61 Waiting >20 seconds for psFlush to relinquish FlushPuck @13:37:11.95 Loading FlushPuck into Collection Clamp from Processing Clamp @13:37:29.80 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @13:38:14.31 Flushing lower manifold's waste1 port @13:38:38.37 Flushing lower manifold's waste2 port @13:39:02.42 Disinfecting external Intake with kill @13:40:35.90 Garaging FlushPuck in Collection Clamp @13:40:49.00 Completed [/home/esp/esp2/mission/2018_08_31_niagara_mission.rb:198] begun 11:30:14 Preparing to sleep until 7:35AM 10/05/2018 Asleep til 7:35AM 10/05/2018 Continuing at 07:35EDT05-Oct-18 @13:40:49.30 Shutdown @07:35:14.54EDT05-Oct-18 Phase [Sample.deep; initialPurge; da withoutWCR] @07:35:16.04 Starting initialPurge Loading FlushPuck into Collection Clamp from FlushPuck::Garage @07:35:32.24 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @07:36:17.88 Purging external Intake @07:36:41.90 Flushing upper manifold @07:37:58.70 Garaging FlushPuck in Collection Clamp @07:38:11.80 da sampling at most 1000ml Loading sh1 puck from tube 6 into Collection Clamp @07:38:40.66 collectSample 1000ml w/bubble point of 24psi Priming 7ml @07:38:40.73 Calibrating pressure and temperature sensors @07:38:54.12 Waiting for external sampler to finish priming... @07:41:54.75 Sampling 1000.0ml @08:07:14.27 Sampled 1000.0ml Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @08:08:01.84 BubblePoint: Intake @14.7psia, Outlet @14.7psia @08:08:22.15 Evacuated: Intake @14.7psia, Outlet @14.7psia @08:08:50.21 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @08:09:36.28 BubblePoint: Intake @14.7psia, Outlet @14.7psia @08:09:56.60 Evacuated: Intake @14.7psia, Outlet @14.7psia @08:10:24.65 Lysing: Adding 2.0ml AqMeOH @08:11:32.48 Heating sh1 puck from tube 6 in Collection Clamp to 70C for 10:00 @08:22:58.48 Collection Puck Heater at 70C after lysing @08:23:39.84 Recovering Lysate into Collection syringe (recycling once) @08:24:21.02 Recycling Lysate (#1 of 1) @08:25:29.24 Recovering Lysate (#2 of 2) @08:26:10.42 Lysing Complete Storing sh1 puck in Collection Clamp to tube 5 @08:26:17.82 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:26:31.93 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:26:34.27 Tranferring DA Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:30:11.35 Garaging FlushPuck in Collection Clamp @08:30:24.45 Loading lyfil puck from tube 6 into Collection Clamp @08:32:54.57 Storing lyfil puck in Collection Clamp to tube 5 @08:33:01.86 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:33:15.97 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:34:18.20 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @08:35:36.76 Garaging FlushPuck in Processing Clamp @08:35:48.61 Loading da puck from tube 6 into Processing Clamp @08:36:09.41 Heating da puck from tube 6 in Processing Clamp to 28C for 2:00:00 @08:43:48.94 recoverPSRreagent08 @08:45:36.14 addPMLReagent08 @08:46:18.21 Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:49:54.77 Garaging FlushPuck in Collection Clamp @08:54:18.21 recoverPRVreagent08 @08:55:18.47 washCycleDA #1 of 2 @08:58:14.11 recoverPSRreagent08 @08:59:24.50 washCycleDA #2 of 2 @09:02:19.40 recoverPSRreagent08 @09:03:29.79 getPMLReagent08 @09:03:52.02 addPMLReagent08 @09:12:33.60 recoverPRVreagent08 @09:13:33.10 washCycleDA #1 of 3 @09:16:28.75 recoverPSRreagent08 @09:17:39.14 washCycleDA #2 of 3 @09:20:34.03 recoverPSRreagent08 @09:21:44.42 washCycleDA #3 of 3 @09:24:39.31 recoverPSRreagent08 @09:25:49.70 Mixing DA Substrates @09:27:00.44 recoverPSRreagent08 @09:28:10.83 Processing Puck Heater at 28C before imaging Imaging da puck in Processing Clamp while storing it to tube 5 @09:28:18.26 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @09:28:35.11 Lights, camera, ACTION! Simulating PuckCamera: snap -bin=2,2 -auto /var/log/esp/da18oct0509h1000ml.tif @09:28:55.11 Waiting >20 seconds for da to relinquish FlushPuck @09:30:25.11 Simulating PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da18oct0509h1000ml15s.tif @09:30:50.11 Simulating PuckCamera: snap -bin=2,2 30.0 /var/log/esp/midres/da18oct0509h1000ml30s.tif @09:31:30.11 Simulating PuckCamera: snap -bin=1,1 70.0 /var/log/esp/hires/da18oct0509h1000ml70s.tif @09:32:51.27 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @09:32:53.61 Starting finalPurge Loading FlushPuck into Processing Clamp from FlushPuck::Garage @09:33:13.61 Waiting >20 seconds for psFlush to relinquish FlushPuck @09:42:11.95 Loading FlushPuck into Collection Clamp from Processing Clamp @09:42:29.80 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @09:43:14.31 Flushing lower manifold's waste1 port @09:43:38.37 Flushing lower manifold's waste2 port @09:44:02.42 Disinfecting external Intake with kill @09:45:35.90 Garaging FlushPuck in Collection Clamp @09:45:49.00 Completed [Sample.deep; initialPurge; da withoutWCR] begun 07:35:14 Preparing to sleep until 11:30AM 10/05/2018 Asleep til 11:30AM 10/05/2018 Continuing at 11:30 @09:45:49.30 Shutdown @11:30:14.54 Phase [/home/esp/esp2/mission/2018_08_31_niagara_mission.rb:207] @11:30:16.04 Starting initialPurge Loading FlushPuck into Collection Clamp from FlushPuck::Garage @11:30:32.24 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @11:31:17.88 Purging external Intake @11:31:41.90 Flushing upper manifold @11:32:58.70 Garaging FlushPuck in Collection Clamp @11:33:11.80 da sampling at most 1000ml Loading sh1 puck from tube 6 into Collection Clamp @11:33:40.94 collectSample 1000ml w/bubble point of 24psi Priming 7ml @11:33:41.01 Calibrating pressure and temperature sensors @11:33:54.40 Waiting for external sampler to finish priming... @11:36:55.04 Sampling 1000.0ml @12:02:14.56 Sampled 1000.0ml Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @12:03:02.12 BubblePoint: Intake @14.7psia, Outlet @14.7psia @12:03:22.44 Evacuated: Intake @14.7psia, Outlet @14.7psia @12:03:50.49 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @12:04:36.56 BubblePoint: Intake @14.7psia, Outlet @14.7psia @12:04:56.88 Evacuated: Intake @14.7psia, Outlet @14.7psia @12:05:24.93 Lysing: Adding 2.0ml AqMeOH @12:06:32.76 Heating sh1 puck from tube 6 in Collection Clamp to 70C for 10:00 @12:17:58.76 Collection Puck Heater at 70C after lysing @12:18:40.12 Recovering Lysate into Collection syringe (recycling once) @12:19:21.30 Recycling Lysate (#1 of 1) @12:20:29.52 Recovering Lysate (#2 of 2) @12:21:10.70 Lysing Complete Storing sh1 puck in Collection Clamp to tube 5 @12:21:17.80 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:21:31.92 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:21:34.25 Tranferring DA Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:25:11.34 Garaging FlushPuck in Collection Clamp @12:25:24.44 Loading lyfil puck from tube 6 into Collection Clamp @12:27:54.85 Storing lyfil puck in Collection Clamp to tube 5 @12:28:01.84 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:28:15.95 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:29:18.18 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @12:30:36.74 Garaging FlushPuck in Processing Clamp @12:30:48.59 Loading da puck from tube 7 into Processing Clamp @12:31:10.66 Heating da puck from tube 7 in Processing Clamp to 28C for 2:00:00 @12:38:50.19 recoverPSRreagent08 @12:40:37.39 addPMLReagent08 @12:41:19.46 Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:44:56.02 Garaging FlushPuck in Collection Clamp @12:49:19.46 recoverPRVreagent08 @12:50:19.72 washCycleDA #1 of 2 @12:53:15.36 recoverPSRreagent08 @12:54:25.75 washCycleDA #2 of 2 @12:57:20.65 recoverPSRreagent08 @12:58:31.04 getPMLReagent08 @12:58:53.27 addPMLReagent08 @13:07:34.85 recoverPRVreagent08 @13:08:34.35 washCycleDA #1 of 3 @13:11:30.00 recoverPSRreagent08 @13:12:40.39 washCycleDA #2 of 3 @13:15:35.28 recoverPSRreagent08 @13:16:45.67 washCycleDA #3 of 3 @13:19:40.56 recoverPSRreagent08 @13:20:50.95 Mixing DA Substrates @13:22:01.69 recoverPSRreagent08 @13:23:12.08 Processing Puck Heater at 28C before imaging Imaging da puck in Processing Clamp while storing it to tube 6 @13:23:28.73 WARNING: Will not reach camera seal! @13:23:28.86 Lights, camera, ACTION! Simulating PuckCamera: snap -bin=2,2 -auto /var/log/esp/da18oct0513h1000ml.tif @13:23:48.86 Waiting >20 seconds for da to relinquish FlushPuck @13:25:18.86 Simulating PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da18oct0513h1000ml15s.tif @13:25:43.86 Simulating PuckCamera: snap -bin=2,2 30.0 /var/log/esp/midres/da18oct0513h1000ml30s.tif @13:26:23.86 Simulating PuckCamera: snap -bin=1,1 70.0 /var/log/esp/hires/da18oct0513h1000ml70s.tif @13:27:45.02 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @13:27:48.08 Starting finalPurge Loading FlushPuck into Processing Clamp from FlushPuck::Garage @13:28:08.08 Waiting >20 seconds for psFlush to relinquish FlushPuck @13:37:06.42 Loading FlushPuck into Collection Clamp from Processing Clamp @13:37:24.26 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @13:38:08.78 Flushing lower manifold's waste1 port @13:38:32.83 Flushing lower manifold's waste2 port @13:38:56.89 Disinfecting external Intake with kill @13:40:30.37 Garaging FlushPuck in Collection Clamp @13:40:43.47 Completed [/home/esp/esp2/mission/2018_08_31_niagara_mission.rb:207] begun 11:30:14 Preparing to sleep until 7:37AM 10/07/2018 Asleep til 7:37AM 10/07/2018 Continuing at 07:37EDT07-Oct-18 @13:40:43.77 Shutdown @07:37:17.70EDT07-Oct-18 Phase [Sample.deep; initialPurge; da withoutWCR] @07:37:19.20 Starting initialPurge Loading FlushPuck into Collection Clamp from FlushPuck::Garage @07:37:35.39 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @07:38:21.04 Purging external Intake @07:38:45.06 Flushing upper manifold @07:40:01.86 Garaging FlushPuck in Collection Clamp @07:40:14.96 da sampling at most 1000ml Loading sh1 puck from tube 7 into Collection Clamp @07:40:45.37 collectSample 1000ml w/bubble point of 24psi Priming 7ml @07:40:45.43 Calibrating pressure and temperature sensors @07:40:58.82 Waiting for external sampler to finish priming... @07:43:59.46 Sampling 1000.0ml @08:09:18.98 Sampled 1000.0ml Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @08:10:06.54 BubblePoint: Intake @14.7psia, Outlet @14.7psia @08:10:26.86 Evacuated: Intake @14.7psia, Outlet @14.7psia @08:10:54.91 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @08:11:40.98 BubblePoint: Intake @14.7psia, Outlet @14.7psia @08:12:01.30 Evacuated: Intake @14.7psia, Outlet @14.7psia @08:12:29.35 Lysing: Adding 2.0ml AqMeOH @08:13:37.18 Heating sh1 puck from tube 7 in Collection Clamp to 70C for 10:00 @08:25:03.18 Collection Puck Heater at 70C after lysing @08:25:44.54 Recovering Lysate into Collection syringe (recycling once) @08:26:25.72 Recycling Lysate (#1 of 1) @08:27:33.95 Recovering Lysate (#2 of 2) @08:28:15.13 Lysing Complete Storing sh1 puck in Collection Clamp to tube 6 @08:28:24.10 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:28:38.21 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:28:41.16 Tranferring DA Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:32:18.24 Garaging FlushPuck in Collection Clamp @08:32:31.34 Loading lyfil puck from tube 7 into Collection Clamp @08:34:59.88 Storing lyfil puck in Collection Clamp to tube 6 @08:35:08.75 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:35:22.86 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:36:25.60 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @08:37:44.16 Garaging FlushPuck in Processing Clamp @08:37:56.01 Loading da puck from tube 7 into Processing Clamp @08:38:15.24 Heating da puck from tube 7 in Processing Clamp to 28C for 2:00:00 @08:45:54.76 recoverPSRreagent08 @08:47:41.96 addPMLReagent08 @08:48:24.04 Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:52:00.60 Garaging FlushPuck in Collection Clamp @08:56:24.04 recoverPRVreagent08 @08:57:24.30 washCycleDA #1 of 2 @09:00:19.94 recoverPSRreagent08 @09:01:30.33 washCycleDA #2 of 2 @09:04:25.22 recoverPSRreagent08 @09:05:35.61 getPMLReagent08 @09:05:57.85 addPMLReagent08 @09:14:39.42 recoverPRVreagent08 @09:15:38.93 washCycleDA #1 of 3 @09:18:34.58 recoverPSRreagent08 @09:19:44.97 washCycleDA #2 of 3 @09:22:39.86 recoverPSRreagent08 @09:23:50.25 washCycleDA #3 of 3 @09:26:45.14 recoverPSRreagent08 @09:27:55.53 Mixing DA Substrates @09:29:06.27 recoverPSRreagent08 @09:30:16.66 Processing Puck Heater at 28C before imaging Imaging da puck in Processing Clamp while storing it to tube 6 @09:30:25.67 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @09:30:42.52 Lights, camera, ACTION! Simulating PuckCamera: snap -bin=2,2 -auto /var/log/esp/da18oct0709h1000ml.tif @09:31:02.52 Waiting >20 seconds for da to relinquish FlushPuck @09:32:32.52 Simulating PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da18oct0709h1000ml15s.tif @09:32:57.52 Simulating PuckCamera: snap -bin=2,2 30.0 /var/log/esp/midres/da18oct0709h1000ml30s.tif @09:33:37.52 Simulating PuckCamera: snap -bin=1,1 70.0 /var/log/esp/hires/da18oct0709h1000ml70s.tif @09:34:58.68 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @09:35:01.44 Starting finalPurge Loading FlushPuck into Processing Clamp from FlushPuck::Garage @09:35:21.44 Waiting >20 seconds for psFlush to relinquish FlushPuck @09:44:19.78 Loading FlushPuck into Collection Clamp from Processing Clamp @09:44:37.62 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @09:45:22.14 Flushing lower manifold's waste1 port @09:45:46.19 Flushing lower manifold's waste2 port @09:46:10.24 Disinfecting external Intake with kill @09:47:43.73 Garaging FlushPuck in Collection Clamp @09:47:56.83 Completed [Sample.deep; initialPurge; da withoutWCR] begun 07:37:17 Preparing to sleep until 11:30AM 10/07/2018 Asleep til 11:30AM 10/07/2018 Continuing at 11:30 @09:47:57.13 Shutdown @11:30:17.70 Phase [/home/esp/esp2/mission/2018_08_31_niagara_mission.rb:216] @11:30:19.20 Starting initialPurge Loading FlushPuck into Collection Clamp from FlushPuck::Garage @11:30:35.39 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @11:31:21.04 Purging external Intake @11:31:45.06 Flushing upper manifold @11:33:01.86 Garaging FlushPuck in Collection Clamp @11:33:14.96 da sampling at most 1000ml Loading sh1 puck from tube 7 into Collection Clamp @11:33:45.66 collectSample 1000ml w/bubble point of 24psi Priming 7ml @11:33:45.73 Calibrating pressure and temperature sensors @11:33:59.12 Waiting for external sampler to finish priming... @11:36:59.75 Sampling 1000.0ml @12:02:19.27 Sampled 1000.0ml Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @12:03:06.84 BubblePoint: Intake @14.7psia, Outlet @14.7psia @12:03:27.15 Evacuated: Intake @14.7psia, Outlet @14.7psia @12:03:55.21 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @12:04:41.28 BubblePoint: Intake @14.7psia, Outlet @14.7psia @12:05:01.60 Evacuated: Intake @14.7psia, Outlet @14.7psia @12:05:29.65 Lysing: Adding 2.0ml AqMeOH @12:06:37.48 Heating sh1 puck from tube 7 in Collection Clamp to 70C for 10:00 @12:18:03.48 Collection Puck Heater at 70C after lysing @12:18:44.84 Recovering Lysate into Collection syringe (recycling once) @12:19:26.02 Recycling Lysate (#1 of 1) @12:20:34.24 Recovering Lysate (#2 of 2) @12:21:15.42 Lysing Complete Storing sh1 puck in Collection Clamp to tube 6 @12:21:24.10 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:21:38.21 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:21:40.86 Tranferring DA Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:25:17.94 Garaging FlushPuck in Collection Clamp @12:25:31.04 Loading lyfil puck from tube 7 into Collection Clamp @12:27:59.88 Storing lyfil puck in Collection Clamp to tube 6 @12:28:08.47 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:28:22.58 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:29:25.02 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @12:30:43.59 Garaging FlushPuck in Processing Clamp @12:30:55.44 Loading da puck from tube 7 into Processing Clamp @12:31:14.95 Heating da puck from tube 7 in Processing Clamp to 28C for 2:00:00 @12:38:54.48 recoverPSRreagent08 @12:40:41.68 addPMLReagent08 @12:41:23.75 Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:45:00.32 Garaging FlushPuck in Collection Clamp @12:49:23.75 recoverPRVreagent08 @12:50:24.02 washCycleDA #1 of 2 @12:53:19.66 recoverPSRreagent08 @12:54:30.05 washCycleDA #2 of 2 @12:57:24.94 recoverPSRreagent08 @12:58:35.33 getPMLReagent08 @12:58:57.57 addPMLReagent08 @13:07:39.14 recoverPRVreagent08 @13:08:38.65 washCycleDA #1 of 3 @13:11:34.30 recoverPSRreagent08 @13:12:44.69 washCycleDA #2 of 3 @13:15:39.58 recoverPSRreagent08 @13:16:49.97 washCycleDA #3 of 3 @13:19:44.86 recoverPSRreagent08 @13:20:55.25 Mixing DA Substrates @13:22:05.99 recoverPSRreagent08 @13:23:16.38 Processing Puck Heater at 28C before imaging Imaging da puck in Processing Clamp while storing it to tube 6 @13:23:25.09 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @13:23:41.94 Lights, camera, ACTION! Simulating PuckCamera: snap -bin=2,2 -auto /var/log/esp/da18oct0713h1000ml.tif @13:24:01.94 Waiting >20 seconds for da to relinquish FlushPuck @13:25:31.94 Simulating PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da18oct0713h1000ml15s.tif @13:25:56.94 Simulating PuckCamera: snap -bin=2,2 30.0 /var/log/esp/midres/da18oct0713h1000ml30s.tif @13:26:36.94 Simulating PuckCamera: snap -bin=1,1 70.0 /var/log/esp/hires/da18oct0713h1000ml70s.tif @13:27:58.10 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @13:28:00.56 Starting finalPurge Loading FlushPuck into Processing Clamp from FlushPuck::Garage @13:28:20.56 Waiting >20 seconds for psFlush to relinquish FlushPuck @13:37:18.91 Loading FlushPuck into Collection Clamp from Processing Clamp @13:37:36.75 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @13:38:21.27 Flushing lower manifold's waste1 port @13:38:45.32 Flushing lower manifold's waste2 port @13:39:09.37 Disinfecting external Intake with kill @13:40:42.86 Garaging FlushPuck in Collection Clamp @13:40:55.96 Completed [/home/esp/esp2/mission/2018_08_31_niagara_mission.rb:216] begun 11:30:17 Preparing to sleep until 7:39AM 10/09/2018 Asleep til 7:39AM 10/09/2018 Continuing at 07:39EDT09-Oct-18 @13:40:56.26 Shutdown @07:39:17.70EDT09-Oct-18 Phase [Sample.deep; initialPurge; da withoutWCR] @07:39:19.20 Starting initialPurge Loading FlushPuck into Collection Clamp from FlushPuck::Garage @07:39:35.39 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @07:40:21.04 Purging external Intake @07:40:45.06 Flushing upper manifold @07:42:01.86 Garaging FlushPuck in Collection Clamp @07:42:14.96 da sampling at most 1000ml Loading sh1 puck from tube 7 into Collection Clamp @07:42:45.96 collectSample 1000ml w/bubble point of 24psi Priming 7ml @07:42:46.03 Calibrating pressure and temperature sensors @07:42:59.42 Waiting for external sampler to finish priming... @07:46:00.05 Sampling 1000.0ml @08:11:19.57 Sampled 1000.0ml Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @08:12:07.13 BubblePoint: Intake @14.7psia, Outlet @14.7psia @08:12:27.45 Evacuated: Intake @14.7psia, Outlet @14.7psia @08:12:55.51 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @08:13:41.58 BubblePoint: Intake @14.7psia, Outlet @14.7psia @08:14:01.89 Evacuated: Intake @14.7psia, Outlet @14.7psia @08:14:29.95 Lysing: Adding 2.0ml AqMeOH @08:15:37.77 Heating sh1 puck from tube 7 in Collection Clamp to 70C for 10:00 @08:27:03.77 Collection Puck Heater at 70C after lysing @08:27:45.14 Recovering Lysate into Collection syringe (recycling once) @08:28:26.32 Recycling Lysate (#1 of 1) @08:29:34.54 Recovering Lysate (#2 of 2) @08:30:15.72 Lysing Complete Storing sh1 puck in Collection Clamp to tube 6 @08:30:24.10 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:30:38.21 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:30:40.57 Tranferring DA Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:34:17.65 Garaging FlushPuck in Collection Clamp @08:34:30.75 Loading lyfil puck from tube 7 into Collection Clamp @08:36:59.88 Storing lyfil puck in Collection Clamp to tube 6 @08:37:08.17 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:37:22.28 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:38:24.51 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @08:39:43.07 Garaging FlushPuck in Processing Clamp @08:39:54.92 Loading da puck from tube 7 into Processing Clamp @08:40:14.74 Heating da puck from tube 7 in Processing Clamp to 28C for 2:00:00 @08:47:54.26 recoverPSRreagent08 @08:49:41.46 addPMLReagent08 @08:50:23.54 Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:54:00.10 Garaging FlushPuck in Collection Clamp @08:58:23.54 recoverPRVreagent08 @08:59:23.80 washCycleDA #1 of 2 @09:02:19.44 recoverPSRreagent08 @09:03:29.83 washCycleDA #2 of 2 @09:06:24.72 recoverPSRreagent08 @09:07:35.11 getPMLReagent08 @09:07:57.35 addPMLReagent08 @09:16:38.92 recoverPRVreagent08 @09:17:38.43 washCycleDA #1 of 3 @09:20:34.08 recoverPSRreagent08 @09:21:44.47 washCycleDA #2 of 3 @09:24:39.36 recoverPSRreagent08 @09:25:49.75 washCycleDA #3 of 3 @09:28:44.64 recoverPSRreagent08 @09:29:55.03 Mixing DA Substrates @09:31:05.77 recoverPSRreagent08 @09:32:16.16 Processing Puck Heater at 28C before imaging Imaging da puck in Processing Clamp while storing it to tube 6 @09:32:24.57 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @09:32:41.42 Lights, camera, ACTION! Simulating PuckCamera: snap -bin=2,2 -auto /var/log/esp/da18oct0909h1000ml.tif @09:33:01.42 Waiting >20 seconds for da to relinquish FlushPuck @09:34:31.42 Simulating PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da18oct0909h1000ml15s.tif @09:34:56.42 Simulating PuckCamera: snap -bin=2,2 30.0 /var/log/esp/midres/da18oct0909h1000ml30s.tif @09:35:36.42 Simulating PuckCamera: snap -bin=1,1 70.0 /var/log/esp/hires/da18oct0909h1000ml70s.tif @09:36:57.58 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @09:36:59.92 Loading FlushPuck into Processing Clamp from FlushPuck::Garage Starting finalPurge @09:37:19.92 Waiting >20 seconds for psFlush to relinquish FlushPuck @09:46:18.27 Loading FlushPuck into Collection Clamp from Processing Clamp @09:46:36.11 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @09:47:20.63 Flushing lower manifold's waste1 port @09:47:44.68 Flushing lower manifold's waste2 port @09:48:08.73 Disinfecting external Intake with kill @09:49:42.22 Garaging FlushPuck in Collection Clamp @09:49:55.32 Completed [Sample.deep; initialPurge; da withoutWCR] begun 07:39:17 Preparing to sleep until 11:30AM 10/09/2018 Asleep til 11:30AM 10/09/2018 Continuing at 11:30 @09:49:55.62 Shutdown @11:30:17.70 Phase [/home/esp/esp2/mission/2018_08_31_niagara_mission.rb:225] @11:30:19.20 Starting initialPurge Loading FlushPuck into Collection Clamp from FlushPuck::Garage @11:30:35.39 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @11:31:21.04 Purging external Intake @11:31:45.06 Flushing upper manifold @11:33:01.86 Garaging FlushPuck in Collection Clamp @11:33:14.96 da sampling at most 1000ml Loading sh1 puck from tube 7 into Collection Clamp @11:33:46.26 collectSample 1000ml w/bubble point of 24psi Priming 7ml @11:33:46.32 Calibrating pressure and temperature sensors @11:33:59.71 Waiting for external sampler to finish priming... @11:37:00.35 Sampling 1000.0ml @12:02:19.87 Sampled 1000.0ml Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @12:03:07.43 BubblePoint: Intake @14.7psia, Outlet @14.7psia @12:03:27.75 Evacuated: Intake @14.7psia, Outlet @14.7psia @12:03:55.80 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @12:04:41.88 BubblePoint: Intake @14.7psia, Outlet @14.7psia @12:05:02.19 Evacuated: Intake @14.7psia, Outlet @14.7psia @12:05:30.25 Lysing: Adding 2.0ml AqMeOH @12:06:38.07 Heating sh1 puck from tube 7 in Collection Clamp to 70C for 10:00 @12:18:04.07 Collection Puck Heater at 70C after lysing @12:18:45.43 Recovering Lysate into Collection syringe (recycling once) @12:19:26.61 Recycling Lysate (#1 of 1) @12:20:34.84 Recovering Lysate (#2 of 2) @12:21:16.02 Lysing Complete Storing sh1 puck in Collection Clamp to tube 6 @12:21:24.10 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:21:38.21 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:21:40.55 Tranferring DA Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:25:17.63 Garaging FlushPuck in Collection Clamp @12:25:30.73 Loading lyfil puck from tube 7 into Collection Clamp @12:28:00.16 Storing lyfil puck in Collection Clamp to tube 6 @12:28:08.15 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:28:22.27 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @12:29:24.49 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @12:30:43.05 Garaging FlushPuck in Processing Clamp @12:30:54.90 Loading da puck from tube 7 into Processing Clamp @12:31:15.02 Heating da puck from tube 7 in Processing Clamp to 28C for 2:00:00 @12:38:54.54 recoverPSRreagent08 @12:40:41.75 addPMLReagent08 @12:41:23.82 Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:45:00.38 Garaging FlushPuck in Collection Clamp @12:49:23.82 recoverPRVreagent08 @12:50:24.08 washCycleDA #1 of 2 @12:53:19.72 recoverPSRreagent08 @12:54:30.11 washCycleDA #2 of 2 @12:57:25.01 recoverPSRreagent08 @12:58:35.40 getPMLReagent08 @12:58:57.63 addPMLReagent08 @13:07:39.20 recoverPRVreagent08 @13:08:38.71 washCycleDA #1 of 3 @13:11:34.36 recoverPSRreagent08 @13:12:44.75 washCycleDA #2 of 3 @13:15:39.64 recoverPSRreagent08 @13:16:50.03 washCycleDA #3 of 3 @13:19:44.92 recoverPSRreagent08 @13:20:55.31 Mixing DA Substrates @13:22:06.05 recoverPSRreagent08 @13:23:16.44 Processing Puck Heater at 28C before imaging Imaging da puck in Processing Clamp while storing it to tube 6 @13:23:24.56 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @13:23:41.41 Lights, camera, ACTION! Simulating PuckCamera: snap -bin=2,2 -auto /var/log/esp/da18oct0913h1000ml.tif @13:24:01.41 Waiting >20 seconds for da to relinquish FlushPuck @13:25:31.41 Simulating PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da18oct0913h1000ml15s.tif @13:25:56.41 Simulating PuckCamera: snap -bin=2,2 30.0 /var/log/esp/midres/da18oct0913h1000ml30s.tif @13:26:36.41 Simulating PuckCamera: snap -bin=1,1 70.0 /var/log/esp/hires/da18oct0913h1000ml70s.tif @13:27:57.57 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @13:27:59.91 Loading FlushPuck into Processing Clamp from FlushPuck::Garage Starting finalPurge @13:28:19.91 Waiting >20 seconds for psFlush to relinquish FlushPuck @13:37:18.25 Loading FlushPuck into Collection Clamp from Processing Clamp @13:37:36.09 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @13:38:20.61 Flushing lower manifold's waste1 port @13:38:44.66 Flushing lower manifold's waste2 port @13:39:08.71 Disinfecting external Intake with kill @13:40:42.20 Garaging FlushPuck in Collection Clamp @13:40:55.30 Completed [/home/esp/esp2/mission/2018_08_31_niagara_mission.rb:225] begun 11:30:17 Preparing to sleep until 7:41AM 10/11/2018 Asleep til 7:41AM 10/11/2018 Continuing at 07:41EDT11-Oct-18 @13:40:55.60 Shutdown @07:41:17.70EDT11-Oct-18 Phase [Sample.deep; initialPurge; da withoutWCR] @07:41:19.20 Starting initialPurge Loading FlushPuck into Collection Clamp from FlushPuck::Garage @07:41:35.39 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @07:42:21.04 Purging external Intake @07:42:45.06 Flushing upper manifold @07:44:01.86 Garaging FlushPuck in Collection Clamp @07:44:14.96 da sampling at most 1000ml Loading sh1 puck from tube 7 into Collection Clamp @07:44:46.55 collectSample 1000ml w/bubble point of 24psi Priming 7ml @07:44:46.62 Calibrating pressure and temperature sensors @07:45:00.01 Waiting for external sampler to finish priming... @07:48:00.65 Sampling 1000.0ml @08:13:20.17 Sampled 1000.0ml Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @08:14:07.73 BubblePoint: Intake @14.7psia, Outlet @14.7psia @08:14:28.05 Evacuated: Intake @14.7psia, Outlet @14.7psia @08:14:56.10 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @08:15:42.17 BubblePoint: Intake @14.7psia, Outlet @14.7psia @08:16:02.49 Evacuated: Intake @14.7psia, Outlet @14.7psia @08:16:30.54 Lysing: Adding 2.0ml AqMeOH @08:17:38.37 Heating sh1 puck from tube 7 in Collection Clamp to 70C for 10:00 @08:29:04.37 Collection Puck Heater at 70C after lysing @08:29:45.73 Recovering Lysate into Collection syringe (recycling once) @08:30:26.91 Recycling Lysate (#1 of 1) @08:31:35.13 Recovering Lysate (#2 of 2) @08:32:16.31 Lysing Complete Storing sh1 puck in Collection Clamp to tube 6 @08:32:24.10 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:32:38.21 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:32:40.55 Tranferring DA Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:36:17.63 Garaging FlushPuck in Collection Clamp @08:36:30.73 Loading lyfil puck from tube 7 into Collection Clamp @08:39:00.46 Storing lyfil puck in Collection Clamp to tube 6 @08:39:08.15 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:39:22.27 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @08:40:24.49 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @08:41:43.05 Garaging FlushPuck in Processing Clamp @08:41:54.90 Loading da puck from tube 7 into Processing Clamp @08:42:15.31 Heating da puck from tube 7 in Processing Clamp to 28C for 2:00:00 @08:49:54.84 recoverPSRreagent08 @08:51:42.04 addPMLReagent08 @08:52:24.11 Beginning samplePassC Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:56:00.68 Garaging FlushPuck in Collection Clamp @09:00:24.11 recoverPRVreagent08 @09:01:24.38 washCycleDA #1 of 2 @09:04:20.02 recoverPSRreagent08 @09:05:30.41 washCycleDA #2 of 2 @09:08:25.30 recoverPSRreagent08 @09:09:35.69 getPMLReagent08 @09:09:57.93 addPMLReagent08 @09:18:39.50 recoverPRVreagent08 @09:19:39.01 washCycleDA #1 of 3 @09:22:34.65 recoverPSRreagent08 @09:23:45.04 washCycleDA #2 of 3 @09:26:39.94 recoverPSRreagent08 @09:27:50.33 washCycleDA #3 of 3 @09:30:45.22 recoverPSRreagent08 @09:31:55.61 Mixing DA Substrates @09:33:06.35 recoverPSRreagent08 @09:34:16.74 Processing Puck Heater at 28C before imaging Imaging da puck in Processing Clamp while storing it to tube 6 @09:34:24.56 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @09:34:41.41 Lights, camera, ACTION! Simulating PuckCamera: snap -bin=2,2 -auto /var/log/esp/da18oct1109h1000ml.tif @09:35:01.41 Waiting >20 seconds for da to relinquish FlushPuck @09:36:31.41 Simulating PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da18oct1109h1000ml15s.tif @09:36:56.41 Simulating PuckCamera: snap -bin=2,2 30.0 /var/log/esp/midres/da18oct1109h1000ml30s.tif @09:37:36.41 Simulating PuckCamera: snap -bin=1,1 70.0 /var/log/esp/hires/da18oct1109h1000ml70s.tif @09:38:57.57 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation @09:38:59.91 Loading FlushPuck into Processing Clamp from FlushPuck::Garage Starting finalPurge @09:39:19.91 Waiting >20 seconds for psFlush to relinquish FlushPuck @09:48:18.25 Loading FlushPuck into Collection Clamp from Processing Clamp @09:48:36.09 Flushing the Collection Valve::Manifold CS Pulling 5ml of flush @09:49:20.61 Flushing lower manifold's waste1 port @09:49:44.66 Flushing lower manifold's waste2 port @09:50:08.71 Disinfecting external Intake with kill @09:51:42.20 Garaging FlushPuck in Collection Clamp @09:51:55.30 Completed [Sample.deep; initialPurge; da withoutWCR] begun 07:41:17 Asleep after completing 2018_08_31_niagara_mission assays